LOCUS       DAOTNG010000001        38070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_1171_length_38070_cov_5.057504, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000001 DAOTNG010000000
VERSION     DAOTNG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 38070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38070
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_38070_cov_5.057504"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="P5179_00005"
     CDS             complement(<1..295)
                     /locus_tag="P5179_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HRS93634.1"
                     /translation="MTYLLDANVFIQAKNLHYGLDFCPAFWEWLTAQNSLGRVFTIDK
                     VADEIAAGGDELTDWMALNGACLVRETDQHVAAQFGRVSDWAKNQQYHPAAVHT"
     gene            complement(292..1443)
                     /locus_tag="P5179_00010"
     CDS             complement(292..1443)
                     /locus_tag="P5179_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRS93635.1"
                     /translation="MDRICVKPELFVWARERAGLDRYALAASFPKLPKWESGELQPTL
                     RQLEAFASAVHVPFGYLFLPDPPDEPLPVADFRSIAGRETARASPNLLDTLYLCQQRQ
                     EWYRDYARLAGLPALSFVGSAGIEDNPVDVAEEMRSTLAISVEERAEIPTWTAALREL
                     VANAEDAGVLVMSSSIVGNNNRRKLQVEEFRGFALADEYAPLVFLNAADSKAALMFTL
                     AHELAHIWLGESGVSDVTAGQVPRQRTERWCNEVAAELLVPMEELRTQFRGGEPLQES
                     IQRLARVFKVSSLVALRRLFDGRYIDETMLWDAYRGELARVRELDRHVTRGGDFYRTL
                     ATRAGRKFACALLASTLEGQTLFRDALRLLGFRKMGTLYNAARELGVTG"
     gene            complement(1549..2514)
                     /locus_tag="P5179_00015"
     CDS             complement(1549..2514)
                     /locus_tag="P5179_00015"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRS93636.1"
                     /translation="MTAVWCVRANAGEYAGHFLRGSYVAIGWCEVAHDLSAVRTREEL
                     YSIMRAAYPDVSSPIVIGNYVGQVARFILEMKAGDYVITPAADTEELHYGRLAPDPSY
                     FFATGDDGCPYRHRRRVEWAPGTLKRSGLSVPLQNTIRSSLTVFAVSQREEFLAAIGS
                     PTQQTATAYDPYRAVLERVLQLDDKEFEILVGHLLTALGFDGSEVTGKTGDGGVDATG
                     ELNVANLAKIKIFVQAKRYKLGSKVSASVVKQLRASIPSNGQGAFITTADFQTSAADV
                     ALQTGFPRIGLVNGRQLVDLLVEHWNDIPADFRDQLGLKPGLVRV"
     gene            complement(2514..3020)
                     /locus_tag="P5179_00020"
     CDS             complement(2514..3020)
                     /locus_tag="P5179_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93637.1"
                     /translation="MARKKSPGSRSIESYDHADKTRTNKTKGISIRAFLVSSHGGFDP
                     VCAEAERWDLRLPPRVSGHFEALKNQGFASLGTHSAASGLNHNSACMNNQVVPLAART
                     ASFETMPASLSCGADHVETESRTLAALRDTLLPQLISGQIRLPTPLCERFAQAGVKGT
                     GRSVGEVV"
     gene            complement(3189..3271)
                     /locus_tag="P5179_00025"
     tRNA            complement(3189..3271)
                     /locus_tag="P5179_00025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3235..3237),aa:Leu,seq:taa)
     gene            complement(3285..4337)
                     /locus_tag="P5179_00030"
     CDS             complement(3285..4337)
                     /locus_tag="P5179_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS93638.1"
                     /translation="MTEPTAPRVFITGGAGFLGINLIRYLLPKGFGIISYDKADFTYP
                     ERDQVTVVTGDIRDFDTLKAAMADSSIVVHCAAALPLYSEKDILSTDIDGTTNVLEAA
                     HQNGIQRVVHVSSTAVYGIPDHHPLREDDPLEGVGPYGIAKVEAEKVCLEYRKKGMCI
                     PIIRPKSFVGPERLGVFALFYEWAKDGKNFPMIGNGRNRYQLLDVEDLCEAIYLCMTG
                     DPAKVNDVFNIGAREFTTMREDYQAVLDRAGFGKRIIGFPAAPVIWTLRFLEMLHLSP
                     LYKWVYETAATDSFVSIEKAERVLGFSPRFSNKQALVRNFEWYLEHLSEFENVTGISH
                     RAPWKQGILKLFKVFF"
     gene            4540..5334
                     /locus_tag="P5179_00035"
     CDS             4540..5334
                     /locus_tag="P5179_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS93639.1"
                     /translation="MAHPPVLERCCYSDVQSLEYEVAVLPIGATEPHGKHLPYGQDFL
                     SVKAIAEEACRRANDGGARTIALPTIPYGVDTNQLGFPFAMSLYQKTLNVIVDDLIES
                     LVCHGVPKLVILNGHGGNEFKSYLREQCRNPRMFVCSINWWTVAEDAYKDIFTHRDDH
                     SGEMETSVALHLMPELVHLERAGDGSTRETDFEGMNSGWVQIARPWHLFTKDSASGDP
                     HLATAEKGVRYAEICISRIAGFLSQLSATPRDDRFPYSRAFATEGR"
     gene            complement(5342..5965)
                     /locus_tag="P5179_00040"
     CDS             complement(5342..5965)
                     /locus_tag="P5179_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS93640.1"
                     /translation="MVFGRLSPRCFSCVFGVALLLLPGLAGAVSYVGLGGGMSPHINT
                     TVVSNYSTGLDTVKFRGGYGFEAQFGWEAAPWIDIEGALRYHLSGKADTLRADREIVS
                     GLDIIGAEGGVRLHPRRGWSNSGPYLRGGIGSFSTTLSLDKGAASQNGEPALGYYAGI
                     GYVHEISGSWGVDLRATYIRFNAFDMNADGRKFRAGFLAVTASLVVF"
     gene            6106..7944
                     /locus_tag="P5179_00045"
     CDS             6106..7944
                     /locus_tag="P5179_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRS93641.1"
                     /translation="MFRKLLCVACAVAVTGCGNTRRTTVVGAASALSYTLSAAKPEGG
                     FGPVFQPYRGVHWTFFGLAILRTAGAPIPNRVETASFLREQPAGPQLSEPDFQRVLAL
                     HMLGFVSDDSTISGLKATQGLDGGWNGSIGATYRAIELLTTLGAKPVFPEKAAQFFVD
                     RLSQDGFFREIPRPGTRSDSLRIASLRNDSTSEASALLWTEHIWNTYCAVQTLTKLGY
                     EVPWRPRVRAWLQSCQNQDGGFSFLPASQVTQSSDVWYAWLAIDALRALGAVPADSLG
                     AVAFINACQNADGGFGDRPGRQSRLESTYYAIAALNSLCGGVEAGLKEKVRVQPPLIR
                     RDGSRDYTANYLYVPSVNRVPSRGFRSLQGRGLPLLAMGDDIVSTDSVRGERAAGANG
                     AQVPGLVETFPRSVIVRLPGNVWAGCRVSFRIGAAQKSVLMGLLRDSESEWAAGTAPS
                     GIPWGVFLERYVTPFVTRGALPVLQIEPGDQAGIYALTEAVGTGLSSDVAVAAALDDG
                     TDLARTCPRIERLNGLVPFTVALGGVSREENARTLWLGNKPNSEGFLEALKNGRAVCA
                     VRTGDSPTGVVFYGDPGVIAFAAAHPQTWAWWDIPAHLRTTHSSVR"
     gene            7992..8975
                     /locus_tag="P5179_00050"
     CDS             7992..8975
                     /locus_tag="P5179_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93642.1"
                     /translation="MKKVRIGFIGCGGVSNGHAYRIAGMPDAQIVALCDTNEATLKTF
                     KERHPELAKTPTFGDWRKMLEAVKLDAIEIHTPHTLHFEQIMAGLDHGLHVLTEKPMV
                     CKTEHARAVVKKAKETGLKVQVSYQRHFDRGFRFAKELIAEGKLGKIQFVAAQQSQEW
                     KRATAGSWRQIPELSGGGQLNDSGSHLVDIVLWVTGLVPEKVTAVIDNCGTPVDINSA
                     VSVQFKGGAQGTFSIVGEGLRWWEDVTIWGEKGIIAFRNGFPTMYVSEDGEVHLPQRM
                     PGGTDPDRNFVDAILGKAELESPAECGLRVIQLTEAAWESAALGKAVKVKR"
     assembly_gap    9111..9210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9211..10597)
                     /locus_tag="P5179_00055"
     CDS             complement(<9211..10597)
                     /locus_tag="P5179_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRS93643.1"
                     /translation="MRTETDALGKLTLDANTRYGIHTARAVASFGPADHPVPRGLVHA
                     YADVKRACARVNHRLGYLTDEQATALEAACSEMAAGLLDEWIVVDAFQGGAGTSTNMN
                     VNEVLANTALLTTGRECGDYGWLHPIDHVNLHQSTNDTYPTALRVAVIRQLRTLSDTL
                     VQLVESFQQAEKRFADVAKVGRTEMQDAVLITLGREMGAYAEAFSRDRWRVFKCEERI
                     RVSNLGGTAVGTGITAPRQFIFQATEELRTITGLPIARAENLVEATQNQDAWVEVAGI
                     LKALASNLIKIGNDLRLLSSGPHAGLREIHLPPMQEGSSLMPGKVNPVIPEMAVQTGM
                     ATFGFEHMISLAVSAGNLELNAFMPLVAYAILTSLDLLERAVDRLARLCVQGIEADRE
                     QCANYVITSHAVATALVPVLGHDATARLVAEAERSGRSLRDVAVSSRALTAEEWEDLT
                     SVERVNALGSPV"
     gene            10732..11799
                     /locus_tag="P5179_00060"
     CDS             10732..11799
                     /locus_tag="P5179_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93644.1"
                     /translation="MPKLPITRRPVKGAVFGFGSIGPVHLYSVRGTPDGSIHPVPDVE
                     VVGVAEPVPERASTIPSGVEVFPDYQTLLRQADVDVVHICLPHHLHAPATIAAAEHGK
                     SVICEKPMALNASEARMMSDAIASNGVGFSLISQNRLNGEKAWMKEKVGAGDLGTLTQ
                     IDWTVDWFRSAEYYDAGSGWRGRDSEARGGVLSNQAYHTLDLVMWLADSPVTEVVAEC
                     STDKVLHPGIDVPDRVEGELHFENGILSRFMCTVCGDKRDVIRVELRGSVDGKDAFIV
                     ADGNSIVEQNLVPESPRFAAEGAVLGKACYGSSHQTNIALSYEAFMGEGTVPVTAETG
                     INVLRVIDAILESNGKPVPVL"
     gene            complement(11842..12903)
                     /locus_tag="P5179_00065"
     CDS             complement(11842..12903)
                     /locus_tag="P5179_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93645.1"
                     /translation="MAKGQTYNYERIPRVDPITGTRYMQLTSFPTINMHLSYCYPNLT
                     NGFTADSRRVVFLSRRAAHRTAPWDVFRVGVDGADFVQLTEREGVRGAVVSSQKPLVY
                     FHSNGTLYSVDMDTFEEEEISHFETDKSQHGGRFSAWLSADERFCFVSAFDKSGYELI
                     VRHATDGSGTAVIASAAEMRIHSIDPAGRGLFVSLNENGRWIIWLVDFDGARVVRYGE
                     NVCSHLSPLGNTGALQGCAVWPEHSLLLLTPGQERENRLLSGPDFWHSSASPDGRWIV
                     ADTNRPNEGIFLVNVATRRSAVLLHPHNVGGDAPWSHAHPFFSPDMRYVAFTTDATGS
                     AQVCLVEIPASLTEQLARP"
     gene            complement(13020..14231)
                     /locus_tag="P5179_00070"
     CDS             complement(13020..14231)
                     /locus_tag="P5179_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRS93646.1"
                     /translation="MVASAGDSLVRQFEFATASRIVFGRGRANEIGSLASDLAGMTGS
                     EKRAFLIIGMTSADGNSGIAAIPEILRSQGFRVAEHRCSGEPTVSAVRLATAAGREHA
                     PHVVVAVGGGSVLDTGKAVAALLSNAGDPEDYLEGVGTGRTFERPAVPLIAVPTTAGT
                     GSEVTRNAVVKADDGSFKKSMRSAFLLPKCALVDSNLTHSLPERSTTYTGMDALTQLI
                     EAFTSNAANPLTDCLAIRGIQLVATSLERVVADGSCADARDDMSLAALLSGICLANAG
                     LGAVHGLVAPLGARFSVPHGAGCAALLAPVIEANSRALRRQGASHPCLEKYRTVEEAF
                     ALRSRAANKGLAQIIRALVTDLRVPGLRYWGVSLEAIPDLVKKSRGGSMRYNPVELGD
                     EELATIIEAAL"
     gene            complement(14225..14533)
                     /locus_tag="P5179_00075"
     CDS             complement(14225..14533)
                     /locus_tag="P5179_00075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRS93647.1"
                     /translation="MYVVCVTVHVKPEHVAEFAAATKTNARSTREEKGNFRFDILQSL
                     ADPAHFLLYEVYESEAAFQEHQKTAHYLSWRDVVADWMAEPRSSVKCAAVFPTEPQEW
                     "
     gene            complement(14545..>15282)
                     /locus_tag="P5179_00080"
     CDS             complement(14545..>15282)
                     /locus_tag="P5179_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93648.1"
                     /translation="SRRYAWVFCPGPVWWQMSDDQWRRHGGPHVAALPLVPEFADYTL
                     PLRQPEIAVGAAYDTMRNAVKTRTSADLLSGLGMPPSWWVNGPYDNSGGKGYDCVLAP
                     EQGIDLDMASLGVWGEVRWKQVRTPPTGYVDLSRLIAGGSEIQGYAATWCETETEISV
                     VVRFGCDDTGKIWCNGNLVHESKTERIAVPDEDTFPLVLPPGRSVFLLKIGNYRGGWG
                     FYFRITDCKGDEVPSLRWDLPLPLNAT"
     assembly_gap    15283..15382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15383..16134)
                     /locus_tag="P5179_00085"
     CDS             complement(<15383..16134)
                     /locus_tag="P5179_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93649.1"
                     /translation="MQRPEIKLVPDPAPERVKVLLFATPPIGYVAETAPDWAEKGFSG
                     FIRPDMMRGWNADVWTLPEGRRIPGNDNPFLQQCRSMNQALAGAGLRDNFMCVPFTVE
                     LPDWFDDERWAAVCENFRQGARFARLAGFRGMALDDEYIEDSLGLNWARYQKPGYPVD
                     KLLPQARFRGQQIQNAMLSEFPDMVTLHLPESYSIMGDLAKALFLGYLDALCAADAPG
                     GLDILPECTYFQTDPWWIALYGAGLDQILREDL"
     gene            16266..17594
                     /locus_tag="P5179_00090"
     CDS             16266..17594
                     /locus_tag="P5179_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS93650.1"
                     /translation="MDTVRIEDDIPVVSRPDVLVAGGGFAGICAAVAAAEQGADVLLV
                     EGFSELGGAMAVSGVSGFCGHTRGVGRVFDEIIAALEEARAITPYHPEQDARAVETVY
                     VGFLMQEYVLRKGVTLLLHSRVAATRRDGNNVDTVFLHTAGGLQAYCPKLIVDATGDA
                     DVAFAAGYPTVSGSESGGAPLPMSLYFSMWDTHCEQTPWLPPGCPRYTAETIPMTSIH
                     RHENGRIDVKMKVIGHSAVNARELSDAEIEARRQMMGLVYFLQTEGYSGRRYSTYCLS
                     SISPLIGIREGRRIIGDVVLTEQDVRAGREWEDAVVVGTYHVDYHWPTVLQRAGTGIT
                     DAVPPYHIPLRALIPRGSENLLVAGRCASGDQMAMSSFRVMATAAGMGIAAGTCGCLA
                     LREGCAPRDVDVGELQDLLRRNGVVLDCSWHSIYQQERRRRAMEAGGKAP"
     gene            17608..17814
                     /locus_tag="P5179_00095"
     CDS             17608..17814
                     /locus_tag="P5179_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93651.1"
                     /translation="MENRDVTDRDRALAQKCVDCPVCSHARKKQRGIAFWFVKHVEGG
                     ICPSCRAYERVYGRKAYEPVPPGE"
     gene            complement(17851..19407)
                     /locus_tag="P5179_00100"
     CDS             complement(17851..19407)
                     /locus_tag="P5179_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93652.1"
                     /translation="MPQTPEWAKAQTSARRVLERWISSSIARNLEIPYHGIHDEGSFT
                     ASWDAFYFTTRDARIRDFLVWLRDGFADWTKDNLLHGYYPEGEVHHATEPFTHFIARF
                     RTLLAGDPLTAHLLEDAAEHVGNWVPEIPAWYDWKAHCMKSWRIGTRVVKTTPPDDYE
                     EPDSVRPAIIALAAYAVTGKERYLAFCCDYADKWAAALLETPLPRVRFLQSAEDLYND
                     RIVHQATGNLQLRLELVVASGLADWLMDLFYLTEKPTYAEASRVVIAGLVPVLADPRN
                     SVAAALVAKYRRVTGDRSLDETIIASLGEPPRYDKAGIVLREDWTDSKETRKGKSMLL
                     NKRIGHRFDQVRWADEEGQEVTEPTGAAWALAWQITGEERYAARAMFLAGERLRLALE
                     KLHDGRDHGCGGNTVGAVASGHGRADRFGHVNSVWGPLVIGSSRVFSAEQPLVIYPFG
                     LPDGVASLVNSAGHAGVEWFNTGEVARTVTWVDGSRPDATPQHVIIQPGERREAPLEK
                     TFPISCAGAG"
     gene            19560..21404
                     /locus_tag="P5179_00105"
     CDS             19560..21404
                     /locus_tag="P5179_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93653.1"
                     /translation="MKSLSRVVPYLLFPAVVLFSCAPKPAVVPAGQQPGVAPAPRTYS
                     YAPDPYPERVKTLVFTTIAPEFLADSAGYFARNGIDGFMVADIMHSWDSDIWKQPTTF
                     TPDEPAGRVVGEGNPLFQLCKRMNEQCRAAGMNSNSIKVAFYRPLPDWFDDAAWDSLC
                     ENFRQCAIFARDAGFAGVALDIEYVYPTYELTYEAYLREGYPRDRLPDQAHQRGYEIM
                     SAMISEFPDMVNWHLPETVWTYGPLGVQLFAGMVDALAEVDAPGGIHMCVENMYDVTD
                     PERILNNTLSIDLAVRKALNQSRDKRAFEYWMRRGSIATGMWPLGYYRMVMDASGRFM
                     GYSGKTETFGDRIVGSYADKSANFPPEEFRRQYAAVRQISREYAWIYGHGPVLWQMSE
                     TARRRYHAAYNDTLPTDVNLEAYLAVLREKPVITDSAFATRAELVRNRFPVVFPGTPP
                     RLWHIGPFPCRKNEFSVVYPPERGVDLAAMYSAYDGYDVPGGDLRWKRVDLDATGFVD
                     LKEIVSGQDSVLAYSVAWVDVPTLTRAELRVGSNDYVAVFVNGREVLRSTRGRVALRD
                     ENVIPVQLTPGWSEIMVKCGNVRRAWGFYLRVTDENGNEVPGLRWVEP"
     gene            complement(21426..22394)
                     /locus_tag="P5179_00110"
     CDS             complement(21426..22394)
                     /locus_tag="P5179_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HRS93654.1"
                     /translation="METEIPVVTKNDLVTGLRSVGLSRGQHVVVHSSLSRFGRVEGGA
                     ETVIAALKEIITPEGTVVMPTFTSRIEFFAEALVARCVAQGKDGFHGTVAELYDTVRQ
                     IWLDAGFQIVPFDTPAALWKRFHDEGVFTHWKNWEISPQIFPLPDDEFVHIVKHGRPL
                     PPDQLHPWCIAGTVGIIPETFRKQPDVRRSQHYSGSFAAWGSMTDFVLKGHDNYSPRV
                     FEDHPLYRMKEIGGKILLLGINHGNNSTIHVAEEAAVRAKGVNVPHEYLGHFMNIEAP
                     LDAAGGQTHGKIGNAEVRLADTRILYRVCAEILDAKLAGGELVPVG"
     gene            complement(22413..24758)
                     /locus_tag="P5179_00115"
     CDS             complement(22413..24758)
                     /locus_tag="P5179_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93655.1"
                     /translation="MPRRQMDIPITHVHHGAYTLEGDIEPIIAELRPAPPVLYQCSST
                     ATPLPNTAGAAIAAPTDPDRFCSPHEIRERLNLARKWVSELHGIGIRWVIPYTCNVKL
                     GGTYEGRKGIWKFYDHWDDYAFLGIGPRPEADPIEWMARERDGQLHYSYEKRHPGFTS
                     CGWYKYTPCLNNPHFNTYQRANVRTIALAGYDGVFVDNNNINCYCQHCEKLFKDWMSK
                     RHTLDQLHERFGWSTYDEVHLGWRGNRMDWVKYDPLFRRFVGDTLTSQEKIKWFETDD
                     MKEIKLQEAGSGWLWGRSNEFLSWVYVRISPEERRKTWGVEHIEDSWGIRDERDRLLW
                     AETSRFWASCIRENLRKIKGYGQEVSGKDFLVVPNWGELLTFQELAFREDMGHDIEEW
                     APATDIVFWEDNRDPGRTAEGLYFDYSLEYKLAHAAGVRSGAMAALPGDHDTCALAHG
                     EAVAHGGAFIQRGPEFADLREDFNRFLSEHRDWFEGHVSAARVGLVLSFQTYRLGGNT
                     HIRDTFRLFQFLNDQQVPFDIIVDTQLPDKVLLRSYSLLVCSELAALSDEATAAFEEY
                     VNGGGILFGCGALGTTDEFGAPRSGFLAGTSGENLIRYNRLNDVLPPGQISREDALEF
                     VTNIWRYLNWSVTKWPLINAGDYAVKIERFVDPGAIGARIPGWSNFQVADPHACNGLR
                     VVPYSKVSGRRRELSVHLLNYNVDLTLPAGARSTEIMRDVAISLPVGHGLVLRDARCA
                     RPGKEVEIVDACVKADRVMFSVPELGVYALVHLTFDNHPTR"
     gene            25094..26770
                     /gene="ilvD"
                     /locus_tag="P5179_00120"
     CDS             25094..26770
                     /gene="ilvD"
                     /locus_tag="P5179_00120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRS93656.1"
                     /translation="MVLNKYSRRITEPLSQAASQAMLYAIGLKEEDLSKPQVGIASMG
                     YDGNPCNMHLNKLAGYAKESVWENGMVGLVFHTIGVSDGIAMGTDGMNYSLQSREIIA
                     DSIQTVVAAHWYDAVIALPGCDKNMPGSIIAMCRLNRPALMIYGGTIAPGYWNGKKLD
                     IVSSFESFGQYLSGQITKEELKNILQHACPGAGACGGMYTANTVASAIETLGMTVPGS
                     SSRPAESPEKTQECRTIGATVRNLIEKDIKPRDIITRKALENAITMVMALGGSTNAVL
                     HLIAIAKAAGVPLTVDDFQRISDRTPYIADLKPSGKYVMEELHTVGGVPAVQKLLLRE
                     GYLDGSCLTVTGKTLAENLAEADDLKPGQTVIMPLSNPIKKTGHIQILYGNLAPGSAV
                     AKITGKEGEVFSGPAKVYDGEEETLRALERDEVKPGDVVVIRYEGPKGGPGMSEMLTV
                     TATIMGKGLGSSVALITDGRFSGGTHGFVVGHVTPEAQDGGPIALVRTGDKITIDARS
                     RQLIVHISDDEMNARRNAWVAPPYKATNGTLRKFIENVSPASEGCVTDQF"
     gene            27267..28319
                     /locus_tag="P5179_00125"
     CDS             27267..28319
                     /locus_tag="P5179_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93657.1"
                     /translation="MTSRERLLTAIHNDTPDRVPVGPFDLGRLPRDSPIARDLIRTTD
                     PFIQCGLGGDPIWGKNCPTERIQDGRDRITIVHTPLGDMRSVWRSTDVASATVEYFCS
                     TPEDAEKWLSIPYTPPEPDLADFYRVKQEIGEEGLVLAGMGDAICLPASLYSPEDFCL
                     LWLDAPDVMRRLVQTAANRINAYIERACAQGMDAFRIVGGEYASVQLGPEAFQELVVE
                     PDRELCDIMRRHGAVAYYHNHGPVTRFYDQFVEIGMHALDPMEAPPWGDCNLEEAKQK
                     LGGKVCLVGNLDDMEIMESLEWSEIEPVASERLLQAGPDGFVLGGTASGTYGEHAARM
                     FMKLVPLAEAMASRWN"
     gene            28356..29642
                     /locus_tag="P5179_00130"
     CDS             28356..29642
                     /locus_tag="P5179_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93658.1"
                     /translation="MVKAGFGLIEISPLVGTPVSGYLNARYASGVHDPLFARALALTD
                     GQTSALLISFDLIGLADADVAAIRKAVAERVPVDEQHIILFCTHTHTGPALVDSFETP
                     RSDEYARSLPERTARAAETAWYDLAEAGVSFAQREVPGVAFQRRYLMKDGTVRTNPGK
                     MNPDIVGPQRQIRTPVTLIGFERDKTRLPIVVASFPCHADVVGGTEVSADYPGRVCDH
                     ITHEFPGHPETLFLRAPAGDINHLNVFDASPQSGYEYAKHMARAITEQAVQAWRERVS
                     TPGPLAIACASVTLERRSVEQSELASARSVFAQSKMLSPLPIDAVWAREKLLLAEMPE
                     QIRVEVNGIRIGGVRILGVPGELFSELGDAIIARSPASNTLVADCCGGKLGYLPTREA
                     YSQNGYEERPARSSPCAPGSGEAVVEAALSLAAELA"
     gene            complement(29668..30444)
                     /locus_tag="P5179_00135"
     CDS             complement(29668..30444)
                     /locus_tag="P5179_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRS93659.1"
                     /translation="MKIYLCTDMEGASGVTMRSEIEPGQERYEFGRRMLTRDVAAAVE
                     GLKAGGATRVVVLDGHGGGDNFLYDELPAGAEYICGRGGDPIPFLDDSFDAVGFVGQH
                     SMAGTPDAVWPHTQSWTDVLGLWANGREIGEIGQFAIFAGHYNLPVIFLSGDRAACAE
                     VRALCGEVQTVAVKQGCSPTRALCLSRAEADKAIYEAARESARGAIRTAPCRVEVPME
                     IRVRFTKTSLADGCVQTGAERVDEVTVRRVVPSALDIYRM"
     gene            complement(30465..32474)
                     /locus_tag="P5179_00140"
     CDS             complement(30465..32474)
                     /locus_tag="P5179_00140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRS93660.1"
                     /translation="MAASNKRRMEAEDLYRFRLISDCRISPDGSSVIYVLNRVERKTE
                     KKFANLWMVPVRGGNPVQFTQGDQIDSSPRWSPDGSRIAFLSNRGGAQNPHLHVIPVG
                     GGEAKKVVDLRGEFGDFVWANDNKTVYFAFRKTDHEVLEREKDETAKKLGVVSRKIER
                     VFFREDGHGFLPKERWHVWSLNTKTGKARQLTHGEIADEVYPSVSTDGERVAFLSNRS
                     HDPDLDPEAVDLFVMPARGGAERRIDTPIGAKSHPVFSPDGRWIAYFGTEGRGAWGQN
                     GKLWVVPSDGSAPPRNLTGEYDVDVPGWTINDLGGAVMMPPVWSPDSAAIYFQVARHG
                     CTNLCRIGIDGSNFETVVAETGVVGAFSFDAGFKRLAFFFANHVEPGQVKVRNLRTGS
                     ERQLTAENSALLQKIAWGTIEEVWFKGADGHNVQGWIAKPPDFDPRKRYPAILEIHGG
                     PHLQYGWFFMHEFHFLAANNYIVFFCNPRGSQGYGEAHARAIENDWGGADYRDLMKWT
                     DFVAEKPYVDSARLGVTGGSYGGFMTNWIIGHTHRFKAAVTQRSVSNMISMYGTSDFN
                     WSFEREMGGRPPWDSVENYWRQSPMKYIGNATTPTLVIHNENDLRCAIEQGEQVFTAL
                     KRLGVETAMIRFPDEFHGMSRNGRTDRRIERLKSILGWFDKYLKQ"
     gene            complement(32577..34175)
                     /gene="pckA"
                     /locus_tag="P5179_00145"
     CDS             complement(32577..34175)
                     /gene="pckA"
                     /locus_tag="P5179_00145"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRS93661.1"
                     /translation="MVGVVPEYQLDQHGFTNLGTVHWNLSTSLLYEEAIRRREGQLAH
                     LGPLVVKTGHHTGRSPNDKFVVQEESTESEIWWGKVNQPYEEKKFHGMYRRLLAYMEG
                     RDVFVQDCAVGADPRYRIPIRIITEDAWQNLFARNMFIQLPREELRTHVPQFTVIAAP
                     RFHAIPEVDGTRSEVFILVNFGKRLVIIGGTAYAGEIKKSVFTFLNYLLPRGDVLSMH
                     CSANVGPDGDTAIFFGLSGTGKTTLSADKGRNLIGDDEHGWSDHGIFNFEGGCYAKMI
                     RLSAEAEPDIYETTRKFGTILENVMMDANTRRLNLDDDGLTENTRGAYPLSHIPGAVV
                     PSVGDHPKNILMLTCDAFGVMPPIAALTADQAGYYFLSGYTAKVAGTEKDMGNEPQAT
                     FSTCFGAPFLALPPTRYADLLGKKVREHKTKCWLVNTGWSGGPFGVGSRMKIQTTRAL
                     IRAALSGKLDGVAMKPDPFFGFLVPESCEGVPSDVLQPRNTWADKEAYDRQAKELAAR
                     FSENFKQYEGMAPREVTESGPKAG"
     assembly_gap    34226..34325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34822..>38070
                     /locus_tag="P5179_00150"
     CDS             34822..>38070
                     /locus_tag="P5179_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS93662.1"
                     /translation="MFRHNVRNPGLRLLAAILPALLTMGRVAAQQEDGEFAFAQHLMR
                     DRMYDLASQQLQQYISNYPNAARTPDAFLLLAEAHAARGAYAKAADTYQGFMIKYPQD
                     VRVRELWLKQAEMRARAGQFAEAARAYTELADSYPESEYADDALIGAASAFLKMNQPD
                     RAERAVNRLITRYPRSPLLSAARALLGRAKLDSGDPQGAQQAVQAVLAERQFSNPVGD
                     AVLVGVEAAIALNKPGEARALSDRLVAQAPGDDRTWLARRTLAEYLLQTGRRTRDAET
                     LNQAADLYKEIARRATTPAAMESALFDLALVRELQEAPALAISNWQDFLQQYPQSRRK
                     PRAMLGLARAHLSGGDSRAGVFALEELLAAYPDSSESTEALGLLGEYYLSRNDPSSAV
                     VYFERQVARTAEGQARRSRLLNVARIRETRLGEPELARTLYGELAVGDDSIAAEGMFG
                     LARCERTLGNLDAAESAYFRVTQQFPAHVLANAAADSLNLIKNYLRIDLQGAIIATMA
                     LEGDQVLVRGNADSLLREKPLRLARIRIEYLKDYPGGIELLKAYLNQPGVSSPDIAEH
                     LLARCYFRLSTRAGLERNVAEQERNRAEGLEALSRLASQYPRSALADDAYIETAEATL
                     AAVDSAARAARAREVYRQFLERYPQSDRRDYALVRIAESTIALARTGSGSPDEALRQF
                     DEALQIAPQGETLDRALFGSSLILMNQGKQDEARLRLERLIAERPLSPLVPESRFQLA
                     GIMYEQRQYRAAARELEKMIRLRQSSRDINEIRTRLVLAYEAVGEFESAARVSQEITK
                     AANADAAAWGARHLIAAYMNMRRFAEAEAALGEELALRPNAPDADSLTIVRGKILMER
                     NQSGKAMAVLTGFERNYPRSKFVPEAWRMLADVQFAQGSSEEALANYRKTIQARPSDK
                     QARLGEVAALYRLGRTSEAQGRERALRDLGPITEDDEVRLAIEQGHALARSNDYVGAI
                     NAFARVVERNPRSEWADDALLAQGRVAISSGRLEPAVQAFERLAQQYPDSPLRQEALF
                     ELANAYFQAEFYDQAADAYNRMLEQDTTSQFAAQAMWNLILSYEQIQRLDSAV"
BASE COUNT         7639 a        10905 c        11335 g         7891 t
ORIGIN      
        1 acgtgtggac cgccgccggg tggtattgct ggtttttcgc ccaatcgctc accctgccga
       61 actgagccgc cacgtgctgg tctgtttccc gaacgagaca cgccccattc agtgccatcc
      121 agtcagtgag ttcgtctcca cctgctgcga tctcatcagc caccttgtcg atcgtgaaca
      181 ctcgacccag ggagttctgc gcggttaacc attcccagaa cgcaggacag aagtccagcc
      241 cgtagtgaag gttcttggcc tggatgaaca cgttcgcatc aagaaggtat gtcacccggt
      301 aacccccaac tcgcgcgccg cgttataaag agtgcccatt ttgcggaatc ccagcaagcg
      361 cagggcgtcg cggaacagcg tctggccctc aagggtgctg gccaacaggg cgcaggcgaa
      421 cttccggccg gcgcgagtgg caagcgtacg gtagaaatca ccaccgcgcg tcacgtgccg
      481 gtccagttca cgcactctgg ccagttcccc gcggtaggcg tcccaaagca tcgtctcatc
      541 gatgtaacgt ccatcgaaca ggcgacgaag cgccacaagc gaactgactt tgaacacgcg
      601 agcgagccgc tggatcgatt cttgcagagg ttccccaccg cgaaactggg tgcgcagctc
      661 ctccattggc accagcagtt ccgcagcgac ctcgttgcac caacgttctg tacgctggcg
      721 tggcacttgc cctgctgtga catcggacac cccgctctca cctagccaga tgtgcgcgag
      781 ttcgtgcgcc aatgtgaaca tcagggctgc cttgctgtcc gcagcattga gaaacaccaa
      841 tggagcgtac tcatccgcca gagcgaaacc acgaaactcc tcgacttgga gctttcgtcg
      901 gttgttgttg cccacgatgg agctcgacat caccagcaca ccagcatctt cggcgttggc
      961 gactaactcc cgcagggcgg cggtccaagt ggggatctcc gcccgttcct caacagagat
     1021 tgcgagggta ctccgcattt cttcggctac gtcgactgga ttgtcttcga tacctgcgct
     1081 acccacgaaa ctcaacgcgg gcaagcctgc taagcgcgcg taatccctgt accattcctg
     1141 gcgctgctga caaaggtaga gtgtatccaa gagattcggg ctggcgcgcg cggtctcccg
     1201 gcccgcaatc gaccggaaat ctgcaaccgg caagggttca tcaggtggat caggcagaaa
     1261 caggtacccg aacggaacgt gaactgcgct ggcgaaggct tccagttgcc tcaacgtggg
     1321 ctggagttcg ccgctttccc atttgggcag tttcgggaaa cttgcagcca acgcgtacct
     1381 gtcgaggccc gcgcgttcac gcgcccagac gaacagttca ggtttgacac agatgcggtc
     1441 catacaaatc aatctaacaa tttctccccg tccttcaccc ctatctccgg cggaccggct
     1501 tcggcggcgg tgcctcgtct tccacgacag actcaacgaa actgccgctc ataccctcac
     1561 cagccccggt ttgagtccga gttgatcccg aaaatctgcc gggatatcgt tccagtgctc
     1621 aaccagtaag tcaacgagct ggcgaccatt gacgaggccg atgcgaggga acccggtttg
     1681 tagcgccacg tcggcggcag atgtctggaa gtccgcagta gtgataaacg cgccctggcc
     1741 gtttgacgga atcgaagcgc gcaactgctt gacgacactg gcggaaacct tcgagccgag
     1801 tttgtaccgc tttgcctgca cgaatatctt gattttcgcc agattggcga cattgagctc
     1861 ccccgtggcg tccaccccgc cgtcgccggt ttttccagtg acttcggagc cgtcgaagcc
     1921 cagcgcggta agcaggtgtc ccaccagtat ttcgaattcc ttgtcgtcga gctggagcac
     1981 tcgctcgagt acggcgcgat aagggtcata tgcggttgcg gtctgttggg tggggctgcc
     2041 gatggctgcc agaaactcct cgcgctgcga caccgcaaac accgtgaggg aggagcggat
     2101 cgtgttctgc aacggcaccg aaaggccact gcgcttgagc gttcccggtg cccactctac
     2161 gcgccggcgg tgcctgtaag ggcagccatc gtcaccggtc gcaaagaagt aggagggatc
     2221 gggtgcgagc cgaccgtagt gcagctcttc ggtgtccgcg gccggcgtga tcacgtaatc
     2281 tccggccttc atttcgagga tgaagcgcgc tacctggccc acgtagttgc cgatgacgat
     2341 ggggctgctc acgtccgggt acgcggcccg catgatcgag tagagttctt cgcgtgttcg
     2401 caccgcggac aggtcgtgtg cgacctcaca ccagccgata gcgacgtagg aaccgcgcag
     2461 aaagtgcccg gcgtattcgc cagcattcgc gcgcacacac catacggcag tcatcacacc
     2521 acctccccca cagaccgccc cgtacctttc acccctgcct gagcgaagcg ctcgcagagc
     2581 ggggtaggca gccttatctg ccccgaaatc aactgcggca gcaacgtgtc gcgcagcgcg
     2641 gcgagggtgc gggattctgt ttccacatgg tctgcgccgc atgagagact cgcaggcatg
     2701 gtctcgaagg aggcagtccg cgcggccaga ggcaccactt ggttgttcat gcatgctgag
     2761 ttgtggttca ggccggacgc ggcggaatgc gtaccgaggg aggcgaaacc ctggtttttg
     2821 agggcctcaa aatgaccgga gacgcgaggc ggcagccgaa ggtcccacct ttccgcctcg
     2881 gcgcaaaccg ggtcaaaacc cccgtgcgac gacaccaaga acgcccgaat cgaaatcccc
     2941 ttcgttttgt tcgtgcgcgt cttgtcggcg tggtcgtagg attcaatcga gcgggaaccg
     3001 ggggacttct tccgtgccat caggggtctc ctcgctggtg cgttttccat acggttagat
     3061 agtactcaat cccgctttca aatcaaccca atgggcggtg cactccgaga agcaagggcg
     3121 cggttgaagg gacaagcggg gtgttgtgct ggacgaaggg aattagtgcg cgggagtgta
     3181 gtggtgagtg ccccggtcgg gactcgaacc cgaacggctt tgggccagcg gattttaagt
     3241 ccgcagcgtc taccagtttc gccaccgggg cggttgcgaa aagctcagaa aaacaccttg
     3301 aagagcttga gaatcccctg tttccacggg gcgcggtgac tgattccggt cacgttttcg
     3361 aattcgctca ggtgctcgag ataccattcg aaatttcgca caagcgcctg cttgtttgaa
     3421 aaccgtggtg aaaaaccgag aacgcgttct gctttctcga tggagacgaa ggagtcggtt
     3481 gccgcggttt cgtacaccca cttgtacagg ggagacaggt ggagcatctc cagaaaccgg
     3541 agagtccaga tcaccggtgc ggccgggaat ccgatgattc gcttcccgaa acccgcccga
     3601 tcgagcacgg cctggtaatc ctcccgcatg gtggtgaact ccctggcgcc gatgttgaac
     3661 acgtcgttca cctttgccgg gtcgccggtc atacacaggt agatcgcctc gcacaggtct
     3721 tccacgtcga ggagctggta cctgttcctg ccgtttccga tcatcgggaa gttcttgccg
     3781 tcttttgccc attcgtagaa gagcgcgaac acgccgaggc gttccgggcc gacgaatgat
     3841 ttggggcgga tgatcggtat gcacatgccc ttcttgcggt actccagaca caccttttcc
     3901 gcttcgactt ttgctatgcc gtagggaccg acgccttcca ggggatcatc ttcgcggagc
     3961 gggtgatggt caggtatgcc gtagaccgcg gtggaagaga cgtggacaac ccgttggatg
     4021 ccgttctgat gggcggcttc cagaacgttt gtggttccgt caatatcggt cgagaggatg
     4081 tctttttctg agtacagggg gagagcggcg gcgcagtgga cgacgatgga agagtcggcc
     4141 atcgcggctt tcaacgtgtc gaagtcgcga atgtcgcccg taacgacagt gacctggtcg
     4201 cgttcggggt acgtgaaatc cgctttgtca tacgaaatga tcccgaaacc cttcgggagc
     4261 aagtagcgga tcaggttgat cccgagaaaa cctgcgcctc cggtgatgaa tacgcgtggg
     4321 gctgtgggtt ccgtcatgct tcgttccgtc tggcgggtgt gatgtgcggt gcgtggatat
     4381 ccggacgatt attcgtgttc agaatcgaat tcagaactga aatatcggcc cgcgggcttt
     4441 cgtttggcaa cggaatggca ccggtggaac gcaccgggtg aggccggtat ctttggaagg
     4501 aaatacccat gcgtgctttt gagacacagg gaggaagaaa tggcccaccc accagtcctt
     4561 gaacgatgct gttattcgga tgtacagtca ctcgaatacg aagttgcggt tctgccgatt
     4621 ggcgcaacag agccccacgg caaacacctc ccctacggcc aggatttcct gtctgtgaag
     4681 gcgattgcgg aggaggcgtg tcgaagggcc aacgatggcg gggcgcgcac cattgcgttg
     4741 ccgacgatcc cgtacggtgt ggatacgaac cagctcggtt tcccgtttgc gatgagtctt
     4801 tatcagaaaa cgttgaatgt catcgtggac gatctcatcg agtccctcgt ctgccatggt
     4861 gttccaaagc tcgtgattct taacgggcac ggcgggaacg agttcaaaag ctatctgcgc
     4921 gagcaatgcc gcaatccccg gatgttcgtg tgcagcatca actggtggac ggtggctgag
     4981 gacgcgtaca aggatatctt cacgcaccgt gatgaccatt caggagagat ggaaacgagc
     5041 gtagccctgc accttatgcc tgagcttgtc catctggagc gcgcgggaga tggatccacg
     5101 cgagagacgg acttcgaagg gatgaactcc ggctgggttc agatcgcccg tccctggcac
     5161 ctgttcacga aagattcagc ctcgggagac cctcatcttg ccacggcaga aaagggcgtc
     5221 cggtacgcgg agatatgtat ctcgcgaatt gcgggcttcc tgtcgcagct ttcggctacc
     5281 ccgcgtgacg accggttccc ttactcgcgg gcattcgcga cggagggaag gtagagaggg
     5341 gtcagaatac gaccagcgaa gctgtgacgg cgagaaaccc cgcgcgaaac ttccgtccgt
     5401 cggcgttcat gtcaaacgcg ttgaaccgga tgtacgttgc tcgcagatca acgccccagg
     5461 agccgctgat ttcgtgcacg tagccgatac cggcgtagta tccgagcgcc ggctcgccgt
     5521 tctgtgatgc cgcgcccttg tcgagactca gcgttgtgga gaaagagccg attccccctc
     5581 gcagatacgg tccgctgttt gaccagccac ggcgggggtg gagcctgaca cctccttcgg
     5641 cgcctatgat gtcgaggccg ctgacgattt cgcgatccgc acgcagggtg tcggcttttc
     5701 ccgagaggtg ataccgcagc gcgccctcga tatctatcca cggtgcggct tcccagccga
     5761 attgcgcttc aaaaccgtac cctccacgga atttgacggt atccaaccct gtcgagtagt
     5821 tggaaacgac ggtcgtattg atgtgcggag acataccacc gccgaggccg acataagaga
     5881 ccgcgcccgc cagccccgga agaagcaaca gcgcgacgcc gaacacgcag gagaaacagc
     5941 gaggggatag tctgccgaaa accacgctcc gatcctttcc aaggttgcca gcgcaaggga
     6001 gacaataaca aaatgacgca ctgccgtggc gcgtttacaa cggcgcacgg ttgcggttcc
     6061 ccggtgaagc tcgtacgctt ggtatgattt ctggagcacc tccgcatgtt tcgaaaactg
     6121 ctttgcgtcg cctgcgcagt tgccgtcacc gggtgtggaa acacacgccg gaccacggtc
     6181 gtcggcgcag catccgcgct ctcttatacg ctgtccgcgg cgaaaccgga aggcggtttc
     6241 ggtccggtct ttcagcctta ccgcggggtg cactggacgt tcttcggact ggcgattctc
     6301 cggaccgcag gcgctccaat tccgaatcgc gtcgaaacgg catcgtttct tcgagaacaa
     6361 cccgccgggc cgcagctttc ggaaccggat tttcagcgcg tgcttgcgct ccacatgctg
     6421 ggattcgttt ccgatgacag caccatctcc ggtttgaaag caacgcaggg actcgacggc
     6481 gggtggaacg gcagcatcgg cgcaacatat cgcgcgatag aactcctgac caccctgggc
     6541 gcgaaacctg tctttcccga aaaggcagcg cagttcttcg tcgacaggct ttctcaggac
     6601 ggctttttcc gggaaattcc ccggccggga acgagatcgg acagtttgcg aatcgcttcg
     6661 cttcgaaacg attcaacgag cgaggcctcg gccttactgt ggacggaaca tatctggaat
     6721 acctattgtg cagttcaaac gctgacgaag ctcgggtacg aggtaccctg gcgcccgcgg
     6781 gtgagagcgt ggctgcagtc ctgccagaac caggatgggg gattctcgtt tcttcccgca
     6841 tcacaagtga cacagtcgag tgatgtttgg tacgcctggc ttgcgattga tgctctccgc
     6901 gcgctggggg ccgttccggc tgacagcctc ggagcagtgg cattcatcaa cgcatgccag
     6961 aacgcggatg gggggttcgg ggatcggccg ggaaggcaat ccaggctgga atcgacgtac
     7021 tatgcgattg cggcgctgaa ttcactttgt ggaggggttg aagccggcct caaggagaag
     7081 gtgcgcgttc agccgccgct gattcggcgt gatggaagcc gggattacac ggccaattat
     7141 ctgtacgtgc cttccgtgaa tcgggtaccg tcgcgggggt tcaggtctct tcaggggcgt
     7201 ggcttgcctc tgttggctat gggcgatgac attgtatcga cggattccgt gcgcggggaa
     7261 agagcggctg gcgcaaacgg cgcgcaggtt ccagggctcg ttgagacgtt tccgcgatct
     7321 gtcatcgttc gacttccggg gaacgtgtgg gccggttgcc gtgtctcgtt ccggattgga
     7381 gccgcgcaga aatccgtttt gatgggactt ctgcgcgatt ctgagagtga gtgggccgcg
     7441 ggaacggctc catcgggaat cccctgggga gtgtttctgg agcggtacgt gacgccgttc
     7501 gttacccgag gcgcactgcc ggttctgcag atcgaaccgg gggatcaggc ggggatctac
     7561 gcgctcacgg aggccgtagg gacgggatta tccagtgatg ttgcagtggc agcggctcta
     7621 gatgatggga ccgaccttgc gcggacttgt cccagaattg aacggttgaa cggtctcgtg
     7681 ccgttcactg ttgcgttagg cggcgtttct cgggaagaga acgctcggac cctctggctc
     7741 ggaaacaaac ccaactcgga aggttttctt gaagctttga agaatggccg cgccgtgtgc
     7801 gcggttcgga caggtgattc acccacgggt gtggttttct acggagatcc cggcgtaatc
     7861 gcatttgcgg ctgcgcatcc gcagacatgg gcctggtggg atataccggc gcatctgcgc
     7921 acaacgcatt ccagcgtccg ttaatgtagg tggttggtcg tcgtacgcgt gttacactct
     7981 ggaggaaaca aatgaaaaag gttcgaatcg ggttcatcgg ttgtggaggt gtgtcaaacg
     8041 ggcatgccta tcgcatagcg ggaatgccgg acgctcagat tgttgcgctg tgcgacacca
     8101 acgaagccac gctgaaaacg ttcaaagaac gccaccctga actggcgaaa accccgacgt
     8161 ttggcgactg gcgcaagatg ctggaagcag tgaagttgga cgctatcgag attcacacac
     8221 ctcacacgct gcattttgaa cagatcatgg cgggtctcga ccacgggctt cacgttttga
     8281 cggaaaagcc catggtctgc aaaacggagc atgctcgagc agttgtgaaa aaggccaagg
     8341 aaacaggtct gaaagttcag gtcagttatc agcgccattt cgatcgaggt tttcgattcg
     8401 ccaaagaact gattgcagag ggcaagctgg gaaagatcca gtttgttgcg gcccagcaat
     8461 cccaggaatg gaagagggca acggccggca gttggagaca gattcccgag ctttcgggtg
     8521 gcggccaatt gaacgattct ggaagccatc tggtagacat tgtgctgtgg gttaccggtc
     8581 tggttcctga gaaggtcacg gcggtgatcg acaactgcgg caccccggtg gacatcaact
     8641 cggcggtgag cgtgcagttc aagggcggtg cccagggcac cttcagcatt gtgggagaag
     8701 ggctccgctg gtgggaagac gtgactatct ggggtgaaaa aggcatcatc gcattccgca
     8761 acggtttccc gacgatgtat gtgagtgagg acggggaggt ccatctcccg cagcgaatgc
     8821 cgggcggaac ggacccggac cggaatttcg tggatgcgat tctggggaag gcggaactgg
     8881 aatctccggc ggagtgtggg cttcgggtga ttcagttgac agaggctgcg tgggaatcgg
     8941 cggctctcgg caaggccgtc aaagtgaagc gttgatagac ctttggtgcg tatcgggccc
     9001 gcgcgatggc cacggctgtc gcgcgggttt caatcttcct ccctcctttt gcgcgccacc
     9061 ggcgaaccca gggcattgac acgttccaca ctggtaaggt cttcccattc nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaccggcga acccagggca ttgacacgtt
     9241 ccacactggt aaggtcttcc cattcctcgg ccgtaagcgc gcgcgaagaa acggcgacat
     9301 ctctcaggga tcttccggaa cgttccgctt ccgccacgag ccgtgccgtg gcgtcgtgcc
     9361 cgagaacagg aacgagagca gttgcaacgg catggcttgt gattacgtag ttcgcacatt
     9421 gctcgcggtc tgcttcgatc ccctgtacgc agagacgtgc caggcggtcg acggcacgtt
     9481 ccagcagatc gagacttgta aggatggcgt acgcgacgag cggcataaac gcattcagtt
     9541 ccagatttcc cgcggatacc gccaagctta tcatgtgctc gaaaccgaac gtcgccatac
     9601 cggtctggac ggccatctcc ggaatgacgg ggtttacctt tccgggcatg agggatgacc
     9661 cctcctgcat gggcggaaga tggatttccc gcagacctgc gtgcgggccg ctggagagca
     9721 ggcgaaggtc atttccaatc ttgatcaggt tcgatgcgag tgccttgagg attccggcta
     9781 cctcaaccca ggcgtcctga ttctgtgtcg cttcgacaag gttctccgct ctggcgattg
     9841 gtagccccgt gattgttcgc agctcctcag tggcctgaaa gatgaactgc cgcggggcag
     9901 tgatgccggt tccaacggcg gttcctccga ggtttgatac gcggatccgc tcctcgcatt
     9961 tgaacacgcg ccagcggtcg cgcgaaaagg cctccgcata tgcgcccatt tcccgtccga
    10021 gcgtaatcaa gactgcatcc tgcatttcgg ttctgcccac tttggcgacg tcggcgaacc
    10081 gtttttccgc ctgctggaac gattccacca gctgcacgag ggtatcactg agggtgcgga
    10141 gttgccggat gacagcgact cgcagcgcgg ttgggtacgt gtcgttcgtc gattggtgca
    10201 ggttgacgtg gtcgatggga tgaagccagc cgtaatcgcc gcactccctg cccgtggtga
    10261 gcagcgcggt gttggcgaga acctcattca cgttcatgtt ggtgctggtg ccggcaccgc
    10321 cctgaaaggc gtcaacaacg atccactcgt cgagtagtcc ggcggccatc tcgctgcacg
    10381 ctgcctccag agcggtagct tgttcgtcgg tgaggtaacc gaggcgatga ttgacacgag
    10441 cgcatgcgcg cttcacgtcg gcgtacgcat ggacaaggcc ccggggaacg gggtggtccg
    10501 cggggccaaa acttgcgact gcgcgggcag tatggatgcc gtaacgcgtg ttcgcgtcca
    10561 gtgtcagctt cccaagagcg tccgtttcag ttctcatctg tatcgttctc actttcctcc
    10621 aggggtgcgc tgccgccggc ccgcgttgac tcgtacctgc gcgttgtcgt ttttcaaaca
    10681 ggagagcctc gtttcagttc gaatgtcatt ccgcagtttg aaaggattgt catgcccaaa
    10741 ctcccaataa ctcgccgtcc cgtcaagggt gccgtgttcg gttttggttc catcgggccc
    10801 gttcatctct actcagtgcg ggggacgccg gacggcagca ttcaccccgt tcccgacgtg
    10861 gaggtcgttg gggttgcgga accggttccc gagcgtgcgt ctaccatacc atcgggagtt
    10921 gaggtgtttc cggattatca gacactcctc cggcaggcgg atgtcgatgt cgtccacatc
    10981 tgtcttccgc accacctcca cgcgcccgcg acgattgcgg ccgcagaaca tggaaaaagc
    11041 gtgatatgcg aaaaaccgat ggcactgaat gcttcagaag cgcgtatgat gagcgatgcc
    11101 attgcatcca acggcgttgg cttttcattg atcagccaga accggttgaa cggcgaaaag
    11161 gcgtggatga aagagaaagt tggtgcgggc gatctcggga cgctcacgca gattgattgg
    11221 acggtggact ggttccggtc tgcagaatat tacgacgcgg gatcggggtg gcgtggccgg
    11281 gattcagaag cacggggagg agtcctgtcg aatcaggcct accacacact ggatctcgtg
    11341 atgtggcttg cagattcgcc tgtcaccgaa gttgtggctg agtgttccac tgacaaggtt
    11401 cttcaccccg ggatcgacgt tccggaccgg gttgaaggag aactccactt cgagaacggc
    11461 atcctgtcca ggttcatgtg caccgtctgc ggggacaaga gggatgttat ccgcgtcgaa
    11521 ttgcgtggat ccgttgatgg caaggatgcg ttcatcgtcg ccgacggcaa cagcatcgtt
    11581 gaacagaatc ttgtgccgga atcccctcga tttgcggcgg agggtgcggt ccttggaaag
    11641 gcgtgttacg gatcaagtca tcagacgaac atcgcgctgt cgtacgaagc gttcatggga
    11701 gagggaacgg tgcctgtgac cgcggagacc ggaatcaacg ttcttcgcgt tatagacgcg
    11761 atccttgaga gcaacggcaa accggttccc gtgctctgac gcgcggagac gtcatttgga
    11821 gggataccgc ccccaacatt tctacggccg ggcaagttgc tccgtcagcg atgccggaat
    11881 ctccacaagg cacacctggg cgcttcccgt ggcatcggta gtgaacgcga cgtaccgcat
    11941 gtccgggctg aaaaacggat gggcgtgcga ccacggcgcg tctccgccga cgttgtgcgg
    12001 gtgcaacagc accgcacttc gcctggtcgc aacgttcacg aggaaaatcc cttcgttcgg
    12061 ccgatttgta tctgctacga tccaacggcc atccggtgat gccgatgaat gccagaaatc
    12121 gggtccgctg agcaaccggt tctcccgttc ctgtccgggg gtgagcaaca ggagcgaatg
    12181 ctcgggccag acggcacaac cctgcagggc tccagtattc cccagcggac tcaggtgcga
    12241 gcatacgttc tcgccgtacc gcacgacgcg tgcgccgtcg aaatccacca gccagatgat
    12301 ccaccggccg ttttcgttaa gggaaacgaa aagcccgcgt cccgccggat caatactgtg
    12361 gatgcgcatc tcagcggcac ttgctatcac tgcggtcccc gaaccgtccg tcgcgtggcg
    12421 aacgatgagt tcgtatcccg atttgtcgaa cgccgataca aagcagaagc gctcgtcggc
    12481 tgagagccac gcggagaatc ggccgccgtg ctggctttta tctgtttcaa agtgggatat
    12541 ctcttcctct tcgaaggtat ccatgtctac cgaatacaac gttccgtttg aatggaagta
    12601 gacaaggggc ttctgcgacg agacgactgc gccccgcacc ccttcgcgct ctgtgagttg
    12661 aacaaaatca gcaccatcaa cgccgactcg gaacacatcc cacggtgcgg ttctgtgggc
    12721 tgctctcctg ctgagaaaca cgactctgcg ggaatccgcc gtgaaaccgt tcgtaaggtt
    12781 cgggtaacaa tacgacaggt gcatgttgat cgtcgggaaa cttgtcaact gcatgtaacg
    12841 ggtacccgtg attggatcga cccgcggaat gcgctcgtag ttgtaggtct gccctttcgc
    12901 cacagggatc tccttcggtt agcgatcgac ggaacccgcg ttgcccgctc ggaacgggac
    12961 catgccacat tccgaaaaac actcatgttg ttgtatgtac agcgatgcgg ccattctcgt
    13021 tacagcgcag cttcgataat cgttgccagt tcctcgtctc cgagttccac ggggttgtac
    13081 cgcatgctgc cgccgcggct ctttttcacg aggtcgggaa tggcctccag actgacgccc
    13141 cagtaccgca ggccgggaac gcgcagatcc gtgacaaggg cacgaatgat ctgcgcaagg
    13201 cccttattcg cggctctgga acgaagcgcg aacgcctcct cgacggttct gtacttttcc
    13261 aggcacgggt ggctcgcgcc ttgccttcgc agcgcccgag agtttgcctc gattacaggg
    13321 gcaagcaatg ccgcgcaacc ggcaccgtga ggaacggaga aacgcgctcc gagcggcgca
    13381 accagcccgt gaacagcacc gagtcctgcg tttgcgaggc agatgccgct caacaaagcc
    13441 gcgagggaca tgtcgtcgcg ggcgtcggcg caggagccgt ccgccacgac gcgttccaga
    13501 gaggttgcca caagctgaat gccgcggatg gcaagacaat cagtgagagg gtttgcggca
    13561 tttgaggtga aggcttcgat cagttgcgtc agcgcgtcca tccccgtgta ggtggtagag
    13621 cgctccggga gcgaatgagt gagattcgag tccacgagcg cgcatttcgg aagaaggaag
    13681 gcgctgcgca tgcttttctt gaaggaaccg tcgtccgcct tgacgacggc gttcctggtc
    13741 acctcagatc cggttcccgc ggtggtcgga acggcgatca gcggtactgc tggacgttcg
    13801 aacgtccgcc ccgttccgac tccctcgaga tagtcttccg ggtccccagc gttcgaaagc
    13861 aaagccgcga ccgcctttcc cgtgtcgaga acgcttccgc ccccgaccgc gacaaccacg
    13921 tgaggtgcat gttctctgcc cgccgcggtt gccagacgaa cagccgaaac ggtaggctct
    13981 ccgctgcacc gatgctctgc aacgcggaag ccttggcttc ggaggatctc agggatcgcg
    14041 gcaattcccg aattcccgtc tgcagaggtc attccgatga tcaggaaggc ccgtttctcg
    14101 ctgccagtca tccctgccag atcagaggca agagaaccga tctcgttggc gcgcccgcga
    14161 ccaaatacga tgcgcgaggc ggtcgcaaat tcgaattgtc gtacaaggga atctccggct
    14221 gacgctacca ttcctgaggc tccgtgggga acactgccgc gcacttaacg gaggaacgcg
    14281 gttcggccat ccaatcagcg acgacatccc gccacgacag ataatgagca gttttctggt
    14341 gttcctgaaa tgcggcttcc gactcataga cttcgtagag aaggaaatgt gccggatcgg
    14401 ccagagactg gagaatgtcg aaccggaaat tgcccttttc ttctcgtgtt gagcgcgcgt
    14461 tcgttttcgt tgccgcggcg aattcggcga cgtgttcagg tttgacatgt accgtcacgc
    14521 acaccacgta catctcagtg cctcctatgt tgcattcaac gggagcggga gatcccagcg
    14581 gagtgaggga acctcgtctc ccttgcagtc ggtgatccgg aaatagaacc cccagccccc
    14641 gcggtaattg ccgatcttca acaagaatac ggatcgtccg ggcgggagca cgagagggaa
    14701 ggtgtcctcg tcgggtacgg caatgcgctc ggttttcgat tcgtgcacga gattcccgtt
    14761 gcaccatatt tttccggtgt cgtcacaccc gaaacgtacc accactgaga tctccgtttc
    14821 cgtctcgcac cacgttgccg cgtacccctg aatttcgctg cctccggcta tgagccggct
    14881 gaggtccacg tacccggtcg gtggggtacg cacctgtttc catcgcactt cgccccagac
    14941 gccaagggac gccatatcga gatcaatgcc ctgttccgga gcgaggacgc aatcgtagcc
    15001 tttgcccccg gaattgtcgt aagggccatt gacccaccat gagggaggca ttcccaggcc
    15061 ggaaaggagg tctgcagaag ttcgggtctt caccgcgttc cgcatagtgt cgtacgcggc
    15121 gcccacagcg atttccggtt gtctcaacgg caacgtgtag tcggcgaatt ccggcacaag
    15181 gggcaatgcg gcgacgtgtg gtccgccgtg gcgccgccac tggtcgtcgg acatctgcca
    15241 ccagaccggc cccgggcaga acacccacgc gtatcttcga gannnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnagatcttc cctcagaatc tggtcgagac ctgcgccgta
    15421 gagcgcgatc caccagggat cggtctgaaa atatgtgcac tcaggaagta tgtcgagtcc
    15481 tccgggggca tccgcagcgc agagagcgtc aaggtacccc agaaagagcg ctttcgcgag
    15541 gtcacccata atggagtagc tctccggaag atgcagggtg accatatcgg gaaactcgga
    15601 cagcatcgcg ttctggatct gttgtccgcg gaatctcgcc tgtgggagca gcttatcgac
    15661 ggggtaccca ggcttctggt atctggccca gttcaacccg agtgagtctt cgatgtactc
    15721 atcatccaac gccatgccgc ggaacccggc gagtcttgcg aatcgagccc cctgccggaa
    15781 gttctcgcac actgccgccc acctttcgtc gtcgaaccag tccggaagtt cgactgtgaa
    15841 cgggacgcac atgaaattgt cacgcagccc ggcaccggca agagcctgat tcatggaccg
    15901 gcattgctga aggaacgggt tgtcatttcc cggtatccgc cttccctcag ggagggtcca
    15961 cacatccgca ttccagcctc tcatcatgtc tggccgtatg aaacctgaga agcccttttc
    16021 ggcccaatcg ggtgccgtct cggcgacata accgatcggg ggcgtggcaa acagaagaac
    16081 tttgacccgt tcaggggcag ggtccggaac aagcttgatc tcggggcgtt gcatggttcg
    16141 tcctgtgcag tggtaatgcc aaatgagtac cgtttcatag tatcgagtca ggtgtgccgc
    16201 ggagcaacgt cacggcgctg aattcgcacg cagcgaaagt ttctgcaggc gcggagggct
    16261 ttgcgatgga tacggtcaga atcgaggacg atattccagt tgtctctcgt cccgatgtgt
    16321 tggttgccgg gggcggtttt gcggggatct gtgcggcagt ggcggcggcg gaacaggggg
    16381 ctgacgtgct tcttgtcgag ggcttttccg agctcggggg cgccatggcg gtttccggtg
    16441 tgtctggttt ttgcgggcac acgcggggag tcgggcgggt atttgacgag atcattgcgg
    16501 ctctggaaga agccagggcc ataacacctt accacccgga acaggacgca cgtgccgtcg
    16561 aaacggtgta cgtgggtttt ctgatgcagg agtatgtact caggaaaggg gtcacgcttc
    16621 tgttgcacag tcgcgtggcg gccacccgca gggacggcaa caacgtcgac accgtgtttc
    16681 tgcacactgc gggcggcctt caggcgtact gcccgaaatt gatcgtggat gcaacgggag
    16741 acgccgatgt tgcgtttgcg gccggatatc ccacggtgtc gggttctgag tcgggcggcg
    16801 ctccgctccc gatgtcgctg tatttctcca tgtgggatac gcattgcgaa cagacgccct
    16861 ggttgccacc agggtgcccg cggtacactg cggaaaccat cccgatgacc tccattcaca
    16921 ggcacgaaaa cgggcgaatt gacgtcaaaa tgaaggtgat aggccacagc gcggtgaacg
    16981 cgcgtgaact ctcagatgcg gagatagagg cacggcgtca gatgatgggt ctggtttact
    17041 ttctgcagac agaaggctat tccgggagac ggtactccac gtattgtctt tcctccataa
    17101 gccccctcat cgggattcgc gagggaaggc gcatcatcgg ggatgttgtt ctgacggagc
    17161 aggacgtgcg ggcaggaagg gaatgggaag atgctgttgt cgtgggaacg taccacgtgg
    17221 actaccattg gcccaccgtg cttcaacgtg cgggtaccgg aatcaccgac gccgttccgc
    17281 cgtaccacat cccattgagg gctctcatcc cgcgcggttc ggaaaacctt cttgtcgccg
    17341 gtcggtgtgc ctctggtgat caaatggcga tgtcgtcttt ccgtgtgatg gcgaccgcgg
    17401 ccggcatggg aatcgccgcc ggaacgtgcg ggtgcctggc gctgcgggaa ggatgcgcac
    17461 ctcgggacgt agacgtgggc gaattgcagg acctgctgcg acgaaacgga gtcgttctcg
    17521 attgctcctg gcactcgatc tatcagcagg aacgacgacg ccgggccatg gaggccggcg
    17581 gcaaagcgcc atagacaggg atacagcatg gaaaatcggg acgttaccga cagggaccgc
    17641 gctctcgctc agaagtgcgt tgattgtccg gtatgcagcc acgcgagaaa aaagcagcga
    17701 ggcatcgcat tctggttcgt caaacacgtc gaaggcggaa tctgcccctc gtgcagagcc
    17761 tacgaacgcg tatacggccg caaagcgtac gaaccggttc cgccgggcga gtgatcccga
    17821 cagaccgtgg tcaggtcacg ccgggagccg tcatcccgca ccggcacatg agatcgggaa
    17881 tgttttctcc agtggagcct cccgtcgctc tccgggctgg attatcacgt gttgcggagt
    17941 ggcgtcaggc cttgagccat ctacccacgt gacagtacgg gcaacctctc ccgtattgaa
    18001 ccattccact ccggcgtggc ccgccgagtt gaccagggat gcgactccat caggcagtcc
    18061 gaaagggtaa atcaccagcg gctgttcagc ggaaaacacc ctggacgatc cgatcaccag
    18121 aggtccccag actgagttca catgtccgaa ccgatcggct ctcccgtgcc cggaagcgac
    18181 cgcgccgacg gtgttccctc cgcacccatg gtcgcgcccg tcgtggagtt tttccaatgc
    18241 gagccgcagc cgttcaccgg ccaggaacat cgctctcgcg gcgtatcgtt cttcgccggt
    18301 gatctgccac gcgagagccc acgcggcgcc tgtcggttcg gtgacttcct gcccttcttc
    18361 gtctgcccat ctgacctggt caaacctgtg tccgatgcgt ttgttcagca gcatcgactt
    18421 ccctttgcgg gtttccttgg agtccgtcca atcttcgcgc agaacgattc ctgctttgtc
    18481 gtagcgtggg ggttctccga gtgacgcgat aatggtttcg tcgaggcttc tgtctcccgt
    18541 tacccgacgg tattttgcta caagagccgc ggcaaccgag ttgcggggat ctgccagaac
    18601 tggcacgaga ccggctatga cgactcgcga agcctccgcg taggtgggct tttctgtgag
    18661 gtagaaaaga tccatgagcc agtctgcaag ccctgacgcc accacaagtt ccaggcgcag
    18721 ttgcagattt cctgtagcct gatggacgat cctgtcgttg tacaggtcct cggcgctctg
    18781 aagaaatcgc acgcggggaa gaggtgtttc cagcagtgcc gcagcccact tatcggcgta
    18841 atcacagcaa aacgcgagat agcgttcctt tcccgtaacc gcgtatgctg ccagcgcgat
    18901 gatcgcgggt cgaacagaat ccggttcctc gtaatcgtca gggggagtgg ttttgaccac
    18961 ccgcgtaccg atgcgccagc tcttcatgca gtgggctttc cagtcgtacc aggctggaat
    19021 ctcaggtacc cagtttccca cgtgctcggc agcatcttcc agcagatggg cggtcagcgg
    19081 gtcacccgcc agaagcgtcc ggaagcgtgc gatgaaatgc gtgaacggct cggtggcgtg
    19141 gtgaacctcg ccttcggggt aatacccgtg caggagatta tccttcgtcc aatccgcaaa
    19201 tccgtcgcgg agccacacca gaaagtcgcg aatgcgggca tcccgcgtgg tgaagtaaaa
    19261 cgcgtcccac gaggccgtga aactcccttc gtcgtggatg ccgtggtacg ggatttcaag
    19321 gttcctcgcg atggaagacg aaatccagcg ttccagaaca cgtcgggcgc tggtctgggc
    19381 cttcgcccat tcaggagtct gtggcataga gatttccctt cgcaggatgc ggtacccggc
    19441 gtgcgtccca aggttcggga cactgtttga cataagcggc aaggtcgcgg ccgcgttgga
    19501 cggaagagcc gttccgttgt atggttaatg cataatctca tttgccgcaa gaggtggtcg
    19561 tgaaatcgct tagtcgcgtt gttccttacc tgctgtttcc cgccgttgtt cttttttcct
    19621 gcgcgccaaa accggcagtg gtgccagccg gccagcaacc cggggtggct ccggcaccaa
    19681 gaacatactc gtatgctccc gatccatacc ccgagcgcgt gaagacactc gtgttcacga
    19741 cgattgcacc ggaattcctt gctgacagcg ctgggtattt cgcaaggaac gggatcgacg
    19801 ggttcatggt cgccgacatc atgcacagct gggatagcga catttggaaa caaccgacaa
    19861 cgttcacgcc cgatgagccc gcgggtcgtg tcgtcggaga gggtaacccg ttgttccagc
    19921 tctgcaagcg tatgaacgag cagtgcagag cggcgggaat gaattccaac agcatcaagg
    19981 ttgcatttta tcgtcctcta cccgactggt tcgatgacgc ggcatgggac agcctctgcg
    20041 agaacttccg tcagtgcgca atcttcgcgc gagacgccgg tttcgccgga gtggctctgg
    20101 atatcgagta cgtctatccc acatacgaac tgacttatga ggcatacctt cgggaaggat
    20161 accctcgcga tcgattgccg gaccaggcgc atcaacgggg atacgaaatc atgtcagcga
    20221 tgatttcgga attccctgac atggtgaact ggcatctgcc ggaaacggtg tggacgtacg
    20281 gcccgctggg tgtccagctt tttgccggca tggtagatgc tttggcggaa gttgacgcac
    20341 ccgggggcat ccacatgtgt gtggaaaaca tgtatgacgt taccgacccg gagcggatac
    20401 tgaacaacac tttgtccatt gatctggcgg ttcggaaagc gttgaaccag tcgagagaca
    20461 aacgcgcgtt cgaatactgg atgcgacgcg gctcgatcgc aacgggaatg tggccgctcg
    20521 ggtactacag gatggtcatg gatgcgtccg ggcggttcat gggttactca ggcaaaacgg
    20581 agaccttcgg agatcggatc gtgggatcgt acgccgacaa gagcgccaat ttcccgccgg
    20641 aggaattccg caggcagtac gcggctgtgc gacagataag ccgcgagtac gcatggattt
    20701 acgggcatgg gccggttctg tggcagatga gcgagaccgc aaggagaagg taccacgcag
    20761 catacaatga tactctgccg acggatgtca acctcgaggc gtacctggca gttctgcggg
    20821 agaaacccgt gatcaccgac tcggcgttcg ccacgcgcgc cgagctcgtg cggaatcgct
    20881 tccctgtggt gttccccggg actccgccgc gattgtggca tatcggacct ttcccctgcc
    20941 ggaagaacga attttcggtt gtgtaccctc ctgaacgagg cgttgatctg gccgctatgt
    21001 actcggcgta cgatgggtac gacgtgccgg gaggagacct gcggtggaaa cgggtggatc
    21061 tggacgcgac gggattcgtt gatctgaagg aaatcgtgag cgggcaggac agcgtgctcg
    21121 cgtacagcgt cgcgtgggtg gacgtcccga cgttgacccg cgcagagttg cgggtcggga
    21181 gcaacgacta tgttgcggtg tttgtgaacg gaagggaggt gctccggtcg acccgcgggc
    21241 gggtcgcact gcgcgacgaa aacgtcatcc ccgtccaact tactcccggt tggagcgaga
    21301 tcatggtcaa gtgtggtaac gtccggaggg cctggggctt ctaccttcgc gtaaccgacg
    21361 aaaacggcaa tgaggtcccg ggtttgcgat gggtggagcc gtagcgccgg aacgcatcga
    21421 atcatttatc cgacaggaac cagttcccca cccgcaagtt tcgcatccag tatctcggcg
    21481 cagacgcggt aaaggatgcg ggtgtccgcc agtcgcacct ccgcatttcc tatcttcccg
    21541 tgtgtctgcc cgcctgccgc atcaagaggt gcttcaatgt tcatgaagtg cccgaggtat
    21601 tcgtggggaa cgttgacgcc cttcgctctc accgcagctt cttcggcgac gtgaattgtg
    21661 gagttgttgc cgtggtttat tcccagaagg aggatttttc ccccgatttc cttcatccgg
    21721 tataagggat ggtcttcgaa caccctcggc gagtagttgt cgtgcccttt gaggacgaaa
    21781 tccgtcatgg agccccacgc tgcgaacgat cctgaataat gctgactcct gcgcacatcg
    21841 ggttgtttcc tgaacgtctc agggatgatg ccgaccgttc cggcgatgca ccacgggtgc
    21901 aattgatccg gtggcaacgg tcgaccgtgc ttcacgatgt gcacgaactc gtcgtccggc
    21961 aggggaaaaa tctgggggga aatctcccag tttttccagt gagtgaacac accttcgtca
    22021 tggaagcgtt tccagagggc cgcaggcgta tcgaacggaa caatttggaa gcccgcatcc
    22081 aaccagatct gccgaacggt gtcgtaaagt tccgctactg tgccatgaaa accatccttg
    22141 ccctgagcca cacagcgggc gacgagagct tcggcgaaga attcgatacg gcttgtgaag
    22201 gtaggcatga ccacggtccc ttcaggcgtg ataatctctt tgagcgccgc aatcacggtt
    22261 tcggcaccgc cttccaccct gccgaaccgg ctaagggagc tgtggacgac aacatgctgg
    22321 cctctcgaaa gaccaactga gcgcagaccg gtgaccaaat cgtttttcgt tacgacgggg
    22381 atttctgttt ccatccgtgt tccctcagga attcagcgtg tagggtggtt atcgaaagtg
    22441 aggtgtacca acgcgtaaac accgagttca ggcacactga acatcactcg atcagccttg
    22501 acgcacgcgt cgacgatctc cacttctttg ccaggccgag cgcagcgggc atctcgaaga
    22561 acaagtccgt gtccgaccgg aagcgaaata gcgacgtccc gcatgatctc tgtggacctt
    22621 gctcccgcgg gtaaggtcag atcgacgttg taattgagca gatggacact cagctcacgg
    22681 cggcgtccgc tgaccttcga atacggaacc accctcagac cattgcatgc gtggggatcg
    22741 gcaacctgga aattcgacca tcccggaatg cgcgctccaa tagctccggg gtcaacgaat
    22801 cgttcgattt tgaccgcata gtctccggcg ttgatcaggg gccattttgt gacggaccag
    22861 ttaagatatc gccagatgtt tgtaacgaat tccagagcgt cctctcgcga aatctgtccc
    22921 ggtgggagca catcattcaa acggttgtaa cgtatcaggt tctctccaga tgtcccggcg
    22981 agaaatcccg atctcggtgc cccgaattcg tcggtggtgc cgagggctcc gcaaccgaaa
    23041 agaatcccgc cgccattgac gtattcttca aaagctgcag tcgcttcgtc gctcaaagcc
    23101 gcaagctccg aacacacaag cagcgagtag ctgcgaagca gcactttgtc gggaagctgg
    23161 gtatccacga tgatgtcaaa aggcacctgc tggtcgttga ggaactgaaa gaggcggaac
    23221 gtgtcgcgaa tgtgcgtatt tccccccaga cggtacgtct ggaatgaaag gacgagacct
    23281 acccgcgcgg ccgacacatg tccctcaaac cagtctcgat gctccgaaag aaaccggttg
    23341 aagtcttctc tcagatcggc gaattcggga ccgcgctgaa taaacgcccc gccatgggca
    23401 acggcttcac catgcgcgag ggcgcaggta tcgtggtcgc cgggcaacgc cgccattgcg
    23461 ccgcttcgga caccagccgc gtgcgccagt ttgtactcaa ggctgtagtc aaaatagagg
    23521 ccttcagcgg ttctgcccgg atcccggttg tcctcccaga acacaatgtc tgttgcaggc
    23581 gcccactctt caatatcatg ccccatgtct tctcggaagg cgagttcctg gaaggtcagc
    23641 aactctcccc agttgggtac aacaaggaaa tcctttccgg agacctcttg cccgtacccc
    23701 tttatctttc gcaggttctc gcggatgcac gatgcccaga aacgtgacgt ttctgcccac
    23761 aggagccggt cacgctcgtc gcggattccc cacgagtcct cgatgtgttc cacgccccac
    23821 gtctttcggc gttcttccgg agaaatgcga acataaaccc agctcaggaa ttcgttactg
    23881 cgtccccaca gccaaccgct ccctgcctcc tgaagcttga tttccttcat gtcgtccgtc
    23941 tcaaaccact tgattttctc ctgggaggtg agagtgtccc ccacgaacct gcgaaacaga
    24001 gggtcgtact tgacccagtc catccggttt ccgcgccagc cgaggtggac ctcgtcgtac
    24061 gtgctccagc cgaaacgctc atgcaactgg tccagcgtgt gcctcttgct catccaatct
    24121 ttgaacagct tctcgcagtg ctggcagtag cagttgatgt tgttgttatc cacgaacacg
    24181 ccatcgtatc cggccagcgc aatggtgcga acgttggctc gctggtaggt attgaaatgc
    24241 gggttattca ggcagggcgt gtacttgtac cacccgcacg acgtgaagcc ggggtggcgc
    24301 ttctcatagg agtaatgaag ttgtccgtca cgttccctgg ccatccattc gatggggtct
    24361 gcttccggcc gcggaccgat gccgagaaag gcgtaatcat cccagtggtc gtagaacttc
    24421 cagatgccct tccggccttc gtacgttccg cccagcttca cgttacatgt gtacggaatt
    24481 acccaccgga tgccgattcc gtgcagctca cttacccatt tccgcgctag attgaggcgc
    24541 tcccggattt cgtgagggga acagaatcgg tccgggtcgg tgggagcggc gatggcagca
    24601 ccggcggtgt ttggcagcgg tgttgccgtg ctgctgcact ggtaaagaac cggtggcgcc
    24661 ggtctcagtt ccgcgatgat cggctcaatg tcaccctcga gcgtgtacgc gccgtgatgc
    24721 acgtgggtta tcggtatatc catttgcctg cgcggcattt ccgtctccgt catagtcggg
    24781 tttggagaga ggtgctccag gttgtgagag ttcgtggcgc cgtttgtgcc cccttttgag
    24841 cggtcgggac agggacggtt gacgtccgcg agtgatggcg agggggcctt cgctggaaaa
    24901 tgatagggat ggtgggagga tttcccaaac cggcgttcca cgcctcagaa ccggtgacat
    24961 accagcgcgt gcacatgctc ggaagctttt ccggctttga tagcttggat agcggccgga
    25021 tatttgtaca acatggaatc cgcgcgtggc aggcacaact tgtggagctc aatcccctac
    25081 gggaggtcaa accatggtac tgaacaaata cagtcggcgc attacggaac cgctttcgca
    25141 ggcggcttct caggcgatgt tgtacgcaat cgggctcaag gaggaggacc tttccaagcc
    25201 tcaggttggt atcgcgagca tgggatacga tggcaatccc tgcaacatgc atcttaacaa
    25261 gctcgccgga tacgcgaagg aatcagtctg ggaaaacggc atggtcgggc ttgtgttcca
    25321 cacaatcggg gtgagcgatg ggattgcgat gggaaccgac ggtatgaatt actccctcca
    25381 gtcccgggaa atcatcgccg attccatcca gacggtggtt gccgcgcact ggtacgacgc
    25441 agtcattgcc cttccgggat gtgacaaaaa catgcccggc tcgatcatcg cgatgtgcag
    25501 gcttaatcgc cccgccctga tgatttacgg cggaacgata gctccgggat actggaatgg
    25561 taagaagctc gacattgtgt catcattcga gagcttcggg cagtatctct ccggccagat
    25621 caccaaagag gaactcaaaa acatcctcca gcacgcctgc cctggagcag gtgcctgtgg
    25681 agggatgtat actgcgaata ccgtggcctc agcgatcgag acgttgggga tgaccgtgcc
    25741 gggcagttca tcgagaccgg cggagagccc tgagaaaacg caggagtgcc ggacaatagg
    25801 cgcgacagtc aggaacctga tcgaaaagga tatcaagccg agggacatca tcacgagaaa
    25861 agcccttgag aatgcgatca cgatggtcat ggcgctgggc gggtcaacga atgccgtcct
    25921 gcatcttatc gccattgcga aggctgcggg ggttccgttg acggtagatg acttccagcg
    25981 cataagcgac agaaccccgt acatcgcgga tctgaaacca agcgggaaat acgtgatgga
    26041 agaactgcat accgttggag gcgttccggc cgtgcagaag cttcttctga gggaaggata
    26101 tctcgatggc agttgcctga cggtgaccgg aaagacgctc gctgagaatc tcgccgaggc
    26161 agacgatctc aaaccggggc agacggttat catgcctttg agtaacccga taaagaagac
    26221 cgggcacata cagattctct acggtaacct tgcccccgga agtgcggtcg caaagataac
    26281 cggtaaagag ggcgaagtgt tcagcggccc ggcaaaagtg tacgacggag aggaagagac
    26341 tctccgggct ctggaaaggg acgaggtcaa accgggcgac gtcgtggtga tccgttatga
    26401 agggccgaaa ggcggacccg gtatgagcga aatgctgacc gttaccgcca cgatcatggg
    26461 caagggcctt ggaagcagcg tagctctcat caccgacggg cgtttctccg gtgggacgca
    26521 cgggttcgtc gtggggcatg tgacaccgga agcacaggac ggcggaccta tcgcgctggt
    26581 tcggaccggt gacaaaatca ccattgacgc acgttcgagg cagctcatcg tgcacatctc
    26641 cgatgatgag atgaatgctc gtaggaacgc ctgggttgcc ccgccttaca aggcaaccaa
    26701 tggcacactg cgcaaattca tcgaaaacgt ttcgcccgca tcagaaggct gcgtgacgga
    26761 tcagttctga gagcaggact tgaccaggcg tatcccggcg taagacgcac aggatgacga
    26821 ttcacgtcag agtgccgggg cgacgtgttc caaaccgcgc actgccgtac ggaatcgtac
    26881 ggtaaagaag tccgcacacg ggcgttcgga acagcgaatc ggccgcatcc gattagcacg
    26941 ggtatgattg ttgctattgg ttggttcggt ggcttgtctg ccggggaaaa acccttggcg
    27001 cgtatgccgc tttcgtcacc cggattccct tgacggggta cccgcggaaa ctccgcgggc
    27061 gttggcggtg cggaacaggg aacgggtggc ggatgaccaa tttgcgccgg tggacgggag
    27121 gtgtccctcc cttccgccgg cgtttcattt gtgccgctgc tccggaacaa actgtgccta
    27181 cccctcggtt gcgctctaga ggccggatga tttaggttca gattactttt cctctgcttc
    27241 cggcattgtt tcctgcggag cttcccatga cttcccgcga gcgccttctg actgctatcc
    27301 acaatgatac tccagatcgt gtcccggtcg gcccttttga ccttgggcgc cttccacgcg
    27361 attctccgat cgcgcgtgat ctgatacgga ccactgatcc gttcatccag tgcggattgg
    27421 gtggggatcc aatctgggga aaaaactgcc cgaccgagcg aattcaggac ggaagggacc
    27481 gtatcacgat agtccacacg ccgctggggg atatgcgttc cgtctggcgg agtactgacg
    27541 ttgcgtcagc aaccgttgag tacttctgca gcaccccgga agatgcagag aaatggttgt
    27601 cgattcccta cactccgcca gagcccgatc ttgcggattt ctatcgtgtc aaacaggaaa
    27661 taggcgagga gggcctcgtc cttgcgggca tgggcgacgc gatatgtctt cccgcatccc
    27721 tttattcccc cgaggacttc tgtctcctct ggttggacgc tcctgacgtc atgcgcaggc
    27781 ttgtgcagac ggctgcgaac cggatcaacg cgtacataga gcgtgcatgc gcccagggta
    27841 tggatgcgtt cagaatcgtt ggtggcgaat atgccagcgt gcaactgggc cccgaagcgt
    27901 ttcaggaact ggtggtcgaa ccagacaggg agctgtgcga catcatgcgg cgccacggtg
    27961 cggtcgcgta ctatcacaac cacggtccgg tcacgcgctt ctacgaccag ttcgtggaaa
    28021 tcggcatgca cgccctcgat ccgatggaag cacctccctg gggggattgt aacctcgaag
    28081 aagccaaaca gaaactcgga ggcaaggtct gtctcgtcgg caatctggac gacatggaaa
    28141 tcatggaatc gctggagtgg agcgaaatag aacctgtcgc ttcggaacgc cttctgcagg
    28201 cgggtcctga cgggttcgtt ctcgggggta cggcgtcggg cacgtatggc gaacacgcgg
    28261 cgcggatgtt catgaaactc gttccgctcg ccgaagccat ggcttcgcgc tggaactgaa
    28321 ggacgccaaa ttctcgtatt ggaggggttt ctggaatggt caaagccggg ttcggcctta
    28381 tcgaaatcag tccgctcgtc ggcacgcctg tatcggggta tctgaacgcg cgctacgcga
    28441 gcggcgtgca cgatcctctc ttcgcacgcg cactggccct gaccgacggt cagacgtcag
    28501 cactcttaat ctcgtttgat cttatcgggc ttgcggatgc agacgtcgct gccatacgaa
    28561 aggccgttgc ggagcgtgtc cccgttgacg agcaacatat catcctgttt tgcacgcata
    28621 ctcacacagg tcccgcactg gtggactctt ttgagacacc caggagtgac gagtacgcgc
    28681 gctctctccc ggaaagaacc gcacgggcag cggagactgc atggtatgac cttgccgaag
    28741 ccggggtgtc cttcgctcag agagaggtgc ccggagttgc atttcagcgc cgatacctca
    28801 tgaaagatgg gaccgtgcgg acgaaccccg ggaagatgaa ccccgacatc gtgggtcccc
    28861 agcgtcagat tcggacaccg gtaaccctga taggctttga acgtgacaaa acacgactgc
    28921 cgatcgtggt tgccagtttt ccgtgccacg cagacgtggt cggggggacg gaagtgtccg
    28981 ccgattaccc cgggcgggtg tgtgatcaca ttacgcacga attcccgggg catccggaga
    29041 cactgttcct acgtgctccc gcaggggata tcaaccacct gaacgtgttc gacgcgtctc
    29101 ctcagagcgg gtacgagtat gcaaaacaca tggcgagagc gatcaccgag caggccgttc
    29161 aggcatggcg cgagcgtgtt tcaactcccg gccctctggc aatcgcgtgc gcttccgtca
    29221 ccctggaacg ccgttccgtg gaacaaagcg aactcgcttc tgcccgcagc gtgtttgcac
    29281 aatccaagat gctttcgccg ttgccgatcg atgcagtctg ggcgcgcgag aagcttcttc
    29341 ttgcggaaat gccagaacaa atccgcgtag aggtgaacgg aatccgaatc ggcggggtga
    29401 gaatactggg cgtgccggga gagttgtttt cggaactggg ggacgcgatc atcgctcgct
    29461 ctcctgcctc aaacacactc gtggcagact gctgcggggg aaaactcggg tacctcccga
    29521 cgagagaggc gtacagtcag aacgggtacg aggaacgtcc cgcacgatcg agcccttgcg
    29581 caccgggttc gggggaggcg gtcgtcgagg ctgctctgag tcttgcggcc gaactcgcgt
    29641 gaaccgacgg tgttttgttg ctgaaactca catgcggtaa atgtccagcg cggagggtac
    29701 cacacgtcga acggtcactt cgtcaacgcg ttcagcccct gtttggacgc atccgtccgc
    29761 cagagaggtt tttgtgaagc gaacccggat ttccatggga acctccactc tgcaaggtgc
    29821 cgtgcggatt gcgccgcggg cggattcccg agcagcttcg taaatcgctt tgtctgcttc
    29881 agcacgtgac agacagagcg cacgcgtcgg agagcacccc tgcttcacgg cgacggtctg
    29941 cacttccccg cacaatgctc gtacctcggc acacgccgct cgatcgccac tgaggaagat
    30001 gaccggcagg ttgtaatgcc ccgcaaaaat cgcaaattgc cctatctcgc cgatctcacg
    30061 cccgtttgcc caaagaccca gtacgtccgt ccacgactgt gtgtggggcc agactgcgtc
    30121 gggcgtaccc gccatggaat gctgccctac aaaccctacg gcgtcgaatg agtcgtccag
    30181 gaacgggatt ggatcgccac cgcggccgca gatgtattcg gctcccgcgg gcaattcgtc
    30241 gtaaaggaag ttgtctccgc cgccgtgtcc atccagcaca actactcgcg ttgctccgcc
    30301 cgctttcaaa ccttccaccg ccgccgccac gtcgcgagtc aacatgcgcc ttccgaactc
    30361 gtacctttcc tggcccggtt caatctcgga ccgcatcgtc acgccggacg cgccttccat
    30421 gtctgtgcaa aggtatatct tcacgccttt tctccctcaa aacgtcattg cttcaggtat
    30481 ttgtcgaacc agccaagaat gcttttcaat cgctcgattc ggcgatccgt tcgcccattg
    30541 cggctcattc catggaactc atcaggaaaa cggatcatgg cagtttcgac gcccagtcgc
    30601 ttgagggccg tgaacacctg ctcgccctgt tcgatggcgc acctcaggtc attttcgttg
    30661 tggatgacca gcgtcggcgt tgtggcgttc ccgatgtact tcatgggtga ctgcctccag
    30721 taattctcca cactatccca cggggggcga ccacccattt ccctctcgaa gctccagttg
    30781 aaatcgctcg ttccatacat gctgatcatg ttgcttacgc ttcgctgggt aaccgcggct
    30841 ttgaatcggt gcgtgtgacc gatgatccag ttcgtcatga aaccaccgta gctgccgccg
    30901 gttactccga gccttgcgga gtcaacgtaa ggcttttctg caacgaaatc cgtccatttc
    30961 atgaggtccc ggtaatcggc gccaccccag tcattctcga ttgcacgggc atgcgcttcg
    31021 ccgtagccct ggctgccgcg tggattacag aaaaagacga tgtagttgtt ggccgcgaga
    31081 aaatggaact cgtgcatgaa gaaccagccg tactgcagat gcggaccgcc gtgaatctcg
    31141 agaatggccg ggtatctctt tctcgggtcg aaatccggcg gtttggcgat ccaaccctgt
    31201 acgttatggc catcggcgcc tttgaaccat acttcctcaa tggttcccca cgcgattttc
    31261 tggagaagtg ctgaattctc cgccgtgagc tggcgctccg accctgtgcg aaggttccgg
    31321 actttcacct gacccggctc aacgtgattg gcaaagaaga acgccaggcg cttgaacccc
    31381 gcatcaaaag aaaaagcacc taccacaccc gtttccgcaa cgacggtttc gaagttgctc
    31441 ccgtcgatgc cgatacggca gaggttcgtg cacccgtggc gggcgacctg aaagtagatc
    31501 gccgccgaat caggagacca tacaggaggc atcatcaccg cgccgccgag gtcgtttatt
    31561 gtccaccccg ggacgtccac gtcatactct cctgtgaggt tcctgggtgg agccgatcca
    31621 tcacttggga ccacccacaa cttgccgttc tgtccccagg cgcccctgcc ctcggtcccg
    31681 aaatacgcga tccaacgtcc gtcaggcgag aacaccggat ggcttttcgc cccgatgggc
    31741 gtatcgattc tccgttcagc gccgcctcgt gccggcatga cgaacaggtc cactgcctcc
    31801 ggatcgaggt cggggtcatg gctccggttg gaaaggaacg ccacccgttc tccgtccgtc
    31861 gagacgctgg gatagacctc gtcagcgatt tctccatgcg tcagctggcg ggctttccca
    31921 gtctttgtgt tcagcgacca cacatgccag cgttctttgg gaagaaatcc gtggccatct
    31981 tcgcggaaga acacgcgttc gatcttcctt gagacgacgc ccagcttttt cgctgtctca
    32041 tctttctcgc gttcgaggac ttcgtggtcg gttttgcgga atgcgaaata caccgtcttg
    32101 ttatcgtttg cccagacaaa gtcgccgaac tcgccgcgaa gatccacgac ctttttcgct
    32161 tccccgcccc cgacggggat tacatgcaag tgcggatttt gcgcaccgcc gcggtttgac
    32221 aggaacgcaa ttcgcgatcc gtccggggac caccggggac tggaatcgat ctggtcgccc
    32281 tgagtgaact gcacggggtt gccgccacgg accgggacca tccagagatt cgcgaacttc
    32341 ttttccgttt tgcgctcgac ccggttgaga acgtagataa cggaactgcc gtcggggctg
    32401 atccggcagt cactgatgag cctgaagcgg tacaaatctt ccgcttccat tctgcgtttg
    32461 ttgcttgcag ccatgattcc tcccgggcgg tccagcggga accccgacga aacgaaaaac
    32521 gacaggggct ggcgctctcg cttaccaacc cctccgttgc ccagtcaggg aaagtgtcaa
    32581 cccgccttgg gtcccgattc tgtgacttct cgcggagcca ttccttcgta ttgtttgaag
    32641 ttctccgaga agcgagccgc gagttccttc gcctgccggt catacgcttc tttgtcggcc
    32701 cacgtattgc gcggttgcaa cacatccgag gggacgcctt cgcatgactc agggacaagg
    32761 aacccgaaaa acgggtccgg tttcatcgcg acgccgtcca gcttgcctga aagggcggcg
    32821 cggatcagcg cacgggtggt ctggatcttc attcgtgacc ctacgccgaa cggtccgccg
    32881 ctccaccccg tgttcacaag ccagcatttg gtcttgtgtt cgcggacctt ttttccgagc
    32941 aggtccgcat accgtgtcgg cggcagcgcg aggaaaggag cgccaaagca ggtgctgaag
    33001 gttgcctggg gctcgtttcc catatccttt tccgttccgg caaccttggc agtgtatccc
    33061 gaaaggaagt agtaccctgc ctgatccgcg gtcaacgcgg caataggagg cattacgccg
    33121 aaggcgtcgc acgtcagcat caggatgttt ttcggatgat caccgacact tgggacaacg
    33181 gcgcccggaa tgtgactcaa ggggtacgca ccgcgcgtgt tctcagtaag cccatcgtca
    33241 tccagattga gacgacgggt gtttgcatcc atcatgacgt tttcgagaat tgtgccaaac
    33301 tttcgtgttg tttcgtagat gtcaggttcc gcctcggccg aaaggcggat catttttgcg
    33361 tagcagccgc cttcaaaatt gaaaatgccg tggtcgctcc acccgtgctc gtcgtccccg
    33421 atcaggttgc gacctttgtc agccgaaagt gtggtttttc cggtaccgga aagaccgaaa
    33481 aagatggcag tgtctccgtc cggaccgacg ttggctgaac aatgcatgga taacacgtcg
    33541 ccgcgaggca gcaggtaatt caggaacgtg aataccgact tcttgatctc cccggcatag
    33601 gcggttccgc cgataataac gaggcgtttt ccgaagttca cgagaatgaa cacttcagag
    33661 cgcgttccgt cgacttccgg tatcgcgtga aaacgcggag ctgcgatgac cgtaaactgt
    33721 ggtacatggg tgcgcaattc ctcgcgagga agctggataa acatgttgcg tgcaaaaagg
    33781 ttctgccacg cgtcctccgt aataatgcga attggaatcc ggtaacgagg atcggcccca
    33841 accgcgcaat cctggacgaa gacgtcccga ccttccatgt atgcaaggag acggcggtac
    33901 attccgtgaa atttcttctc ttcgtagggt tgattgacct tgccccacca gatttctgat
    33961 tctgtggatt cctcttgcac aacgaacttg tcgttcggtg agcgcccggt atggtgtccc
    34021 gtttttacca ccaggggccc gagatgggcg agttgtcctt ctctccgtct gatggcttcc
    34081 tcgtaaagca atgacgtcga aagattccag tggaccgtcc caaggttcgt gaaaccgtgc
    34141 tgatccagtt gatactcggg aacaacccct accattttcc gttctccttt tccctgtggc
    34201 gcggtcggcg tgattctgtg ctgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34321 nnnnnaccat tttccgttct ccttttccct gtggcgcggt cggcgtgatt ctgtgctgcg
    34381 ttgcttttcg ggcgcaaccg gaccgatgca tcgcgttcgc ctgcaggcga cgatcccggc
    34441 cccctgtccg aaaaaacagc gggaaaaata taccgaaggc gcagcgtgtg gccaacacat
    34501 agccccattc tgacacgcgc ttgccggttc ttctgtgggc gatggggcac ccgcgaaatc
    34561 aggtgtgcct ctctctcttt ctgtggaaca ccggcgttgc tctcgaacag atttccggcc
    34621 gcagagtggg atctgggacg ctgtcccctg cgtatgcaca gttggaacta ctgttcttgc
    34681 cttccgttat gccggcttgc actccgtgta cgcatggcac aatggcgttg atgcaaacgc
    34741 atgataactt ggattttagc gccacgcggg gtacattatc tgccataatg gccgcccgtg
    34801 agaacgttcg aaggaggtac gatgtttcgt cataatgttc gcaacccggg tttgcgacta
    34861 cttgctgcca tcctcccggc gctgctaacc atgggcagag tcgcggcaca acaggaggac
    34921 ggcgaattcg cgtttgcgca gcacctcatg cgcgaccgga tgtatgatct tgccagccag
    34981 caactgcaac aatacatcag caactacccg aacgcggccc gaactcccga tgcgtttctg
    35041 ctcctcgcgg aggctcatgc ggcgcgaggt gcgtatgcga aggcggcgga cacctatcag
    35101 gggttcatga tcaaataccc gcaggatgtt cgtgttcgcg agctttggct gaagcaggcc
    35161 gagatgcgcg cacgtgcagg ccagttcgcc gaagcggcga gagcatacac agagcttgcc
    35221 gattcgtacc ctgaaagcga atatgccgac gacgcactca ttggagcagc gtctgccttt
    35281 ctcaaaatga atcaaccgga cagagcggaa cgcgctgtca atcgtttgat tacccggtat
    35341 cctcgttccc ctttgctgtc cgccgctcga gcgttgcttg gtcgcgccaa actggattct
    35401 ggagacccgc agggcgctca acaggctgtg caggcggttc ttgctgagcg ccagttctcc
    35461 aatcctgtag gcgacgcggt tctggtgggc gtggaagcag caattgccct caacaaaccc
    35521 ggcgaagccc gtgcattgag tgatcgcctg gttgcccagg cgcccggaga cgatcgaacg
    35581 tggctggctc gacgcacact ggcagaatac ctcctccaga cggggcggag gacacgggat
    35641 gccgaaaccc tcaatcaggc ggcggacctt tacaaggaaa tcgcgcgtcg tgcaacgaca
    35701 cctgctgcca tggaatcagc gctcttcgat ctggctctgg tcagggaact gcaggaagcg
    35761 ccggcgctcg cgatcagcaa ctggcaggat tttctgcaac aataccctca aagcaggcgg
    35821 aagccgcgcg cgatgctcgg cctggctcga gcgcatctct ccggaggtga ttcacgcgcc
    35881 ggcgtgttcg ctcttgaaga gcttctcgcg gcctacccgg attccagcga gagcaccgag
    35941 gcgctggggc tgctcgggga gtattacctt tcccggaacg atccatcaag cgccgtggtg
    36001 tacttcgagc gacaagttgc ccgtaccgcc gaaggccagg ccagacgatc gagactcctg
    36061 aacgttgcgc gaatacgtga gactcgcctc ggcgagcccg agcttgcacg cacattgtac
    36121 ggcgagctcg ctgttgggga tgacagcata gcggcagagg ggatgttcgg tctcgcgagg
    36181 tgtgaacgga ctctcggcaa ccttgacgcc gcagaaagcg cctatttccg cgtcacccag
    36241 cagtttccgg ctcacgtcct cgcgaacgcg gcggcggata gcctgaacct catcaagaat
    36301 tacctgcgta tcgatcttca aggtgccatc atcgctacta tggcgctgga aggcgatcag
    36361 gttcttgtcc gagggaacgc ggattctctt ttgcgagaga agcccctgag attggccagg
    36421 attcggatcg aatatctgaa agactatccg ggcggtatcg aactcctgaa ggcgtatttg
    36481 aaccagcccg gcgtctcctc ccctgatatc gccgaacacc tgctcgcgcg ctgctatttt
    36541 cgcctgagca cccgcgcggg attggaacgc aatgtggcgg agcaggaaag gaacagggcg
    36601 gaagggctgg aggcgctatc gcgccttgct tcgcagtacc ctcggagtgc actcgccgac
    36661 gacgcgtaca tcgagactgc tgaagcaacc cttgccgcag tcgattcggc ggcccgcgca
    36721 gcaagggccc gtgaggtata ccgccagttc cttgaacggt atccgcagag cgaccgccgg
    36781 gattacgcac tggtccgcat tgccgaaagc acaatcgccc tcgcgagaac cggctccgga
    36841 tcccccgacg aagcgttgcg gcaattcgat gaagcgctcc agatcgcgcc gcagggtgaa
    36901 actctggatc gtgctctgtt cggctcgtcg ttgatcctga tgaaccaggg gaaacaggat
    36961 gaagcgcggc ttcgactgga gcgattgatc gctgaacgcc cgttgagccc gctggttcct
    37021 gagagcagat tccagttggc cgggatcatg tatgagcagc gccagtaccg agcggccgcc
    37081 cgtgaacttg aaaagatgat tcgtcttcgt cagtcatcgc gtgatattaa cgagatcagg
    37141 actcgtttgg ttctggcgta tgaagcggtc ggggaattcg aatctgccgc gcgggtttct
    37201 caggagatca cgaaagccgc caatgcggac gcagccgcgt ggggagcgag gcacctcatc
    37261 gctgcctaca tgaacatgcg tcgctttgcg gaggccgaag ccgcccttgg cgaggaactc
    37321 gccctgcgcc cgaacgctcc ggatgccgac agccttacga ttgtgcgcgg aaaaatcctg
    37381 atggaacgaa atcagtcggg caaagcaatg gctgttttga cgggttttga aaggaattat
    37441 ccaagaagca agttcgtgcc tgaggcgtgg agaatgctgg cggatgtcca gttcgcgcaa
    37501 ggatcgtctg aagaagcgtt agccaattac cggaaaacga tccaggcgcg accgtccgac
    37561 aagcaggcca ggctgggcga agttgccgca ctgtacaggc tcgggaggac gagcgaggct
    37621 caggggaggg agcgcgcgct ccgcgatctg ggtccaatta ccgaggatga cgaagttcgc
    37681 cttgccatcg agcagggcca cgcgcttgcc cggtcaaacg actatgtcgg cgcgatcaac
    37741 gcattcgctc gggtggttga gaggaatccg cgaagcgaat gggcagacga cgcacttctc
    37801 gctcagggac gcgttgcgat ttccagtgga cgactggaac cggccgtcca ggcgttcgaa
    37861 cggctcgccc agcagtatcc ggacagtccg ttgagacagg aagccctttt tgaacttgcg
    37921 aatgcctatt tccaggcgga gttctatgac caggccgctg acgcgtacaa ccggatgctc
    37981 gagcaggata ctacgagcca gttcgccgct caggcgatgt ggaatcttat tctttcgtac
    38041 gaacagattc agcgacttga ttccgccgtt
//
LOCUS       DAOTNG010000002        35757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_1279_length_35757_cov_5.323679, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000002 DAOTNG010000000
VERSION     DAOTNG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 35757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35757
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_35757_cov_5.323679"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="P5179_00155"
     CDS             <1..1581
                     /locus_tag="P5179_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS93663.1"
                     /translation="LGDYPAAESAVLPLTQETGAGAPNSAFAAHGRFLLGLVCWRTGR
                     YADAIRNLHAVSGSPVVSETTKRDARLLSLASVARVEQVNFAEFDTSQAAFVRDALQL
                     NRAIALFNASQMTAARLVLEQLQRISQDTPFASEVAALLADVKSYERTEIRLAVVTPL
                     SGEDSTSGAALLDGVKFAFERLAPPGLSPLVTKSAATQVETIHAVEGFARDTTVLAVI
                     GPLTTENSIVAGAVANAMGLPLVTPTASGEGVCGIGDNIFQINLTARAQGAFLASVVA
                     DSLRAQAVAILSTYDPHDQALSSEFARVVREKGLIVIAHEYFITGTTDLTRPLSAIRR
                     AALQRTPPPADMDTVHFMPEINSIDAMLVCSSHETDLVAVAAQIPTQKIWTRVVGNAT
                     WGNSSVRQQAGEAAEGVLFATTFDQSYPEARNFAEAYRAERGNEPILATALGYDATSL
                     VLQAISTGARTRPEVRRFLADVKEWPGVTGLITLAPDGCNRNAMVRMIRGGTIRPVGD
                     WQILSRAATQNATPRVGQ"
     gene            1596..2621
                     /gene="larE"
                     /locus_tag="P5179_00160"
     CDS             1596..2621
                     /gene="larE"
                     /locus_tag="P5179_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRS93664.1"
                     /translation="MTLEEKHARLREMLLEAAPVVVAFSGGVDSTFLLAAAHRALGKA
                     KCIGVTAVSESLAPAELDEARQIANQIGAEWTTIQTREMDNPRYRANTNERCYFCKTE
                     LFSTLQTLARERGFATVLDGFNADDTGDWRPGARAGVEQGVKSPLKDAGLTKQDIRDL
                     SRSYGLPTWNKPSFACLASRLPYNTEITPERLSLIASAEAVLRSHGFRAFRARYHGET
                     ARLEIGIDEIPRFQEAMLAQSVRDAVMKAGFAGVTLDLAGFRSGSQNETGGSGIVPLP
                     SAARDGLSIIKKQGFAVAKTRIEGNMIRIIVSWEDFPRILKEETRIPLVAALRGQGYV
                     YVAADIP"
     gene            2679..4364
                     /locus_tag="P5179_00165"
     CDS             2679..4364
                     /locus_tag="P5179_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93665.1"
                     /translation="MRWFAVSLLCLLFHLQATAGDEFEPSDPDEASYQNARAEYAELD
                     SATLGRDRSSTDRPFFGALRNARHPRLAFRSRLTGWDLAASTSPDNFTFNNRIMVRQG
                     GVVAGFITERDHDEPRMMDYRSGTLGFLFHGDSTRVVLGAMAAHVGSGLIVGRQGLNW
                     TGALSFRERETALTPALTTTESGALHGIGFRYVGRVWEILALYTTPRWDATIDSATNS
                     AGAISVTGLHRTDAELRAAGALKETFRLLRIGGNFGRSARVGLTAAKSEFSPRTLYRH
                     SSKATDEISFLGVDAQSTIGSFGVRGEAVLQDEREYGGAGALRFHNESLTMTAAGWLY
                     SSAFTSWHGTGWSLHSEASSERGGALVVEWAPGSGVHVDGFLAHSVEPLDAAGRDFPR
                     WTTVEELRWRTPLSTRFSWIGRWKQKSTRSTVAEGIFMERRRDISSDLELIASGMTFR
                     GGWAFAQNQSGDRGGLVRIRMQSRPARGLDCTLGGAFHRTNSTSLPLYFYEQQVPNYG
                     FLWSLYGSRASWTVRARYHTGRATASLFVLGTGTLNEAADRLTVTAQIDYVLR"
     gene            4413..5534
                     /gene="gcvT"
                     /locus_tag="P5179_00170"
     CDS             4413..5534
                     /gene="gcvT"
                     /locus_tag="P5179_00170"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRS93666.1"
                     /translation="MTTSPVPLLRTPLFNRHLALGARMAPFAGFEMPIMYRSIAEEHN
                     AVRHGCGIFDVSHMGRLRFSGTAGHELLERLTTIHVASVGAGTARYGFILTDSGGILD
                     DVILFATRAAEWVLVVNAGNRRSVVEHMGKLEPHPRFFDDTESTGMVAVQGPATRAVL
                     RQVMPGLAVPDRANRVIETDWNGTPVVLSTTGYTGEDGVEIIAASGRIEALWDSVIEA
                     GAVPAGLGARDTLRLEMGYPLHGHELSKETDPWQAGLGWAIDRRNEKFTGRSAVLARE
                     NDKTPRLVGLKAAGRGIPRAGNLVEDHGIVVGEVTSGTFSPSLQQGIALAYVGFGAAS
                     PGKQFSVRDRESESRRITMEQVLAPFYRTGTRRSARNAS"
     gene            5531..6250
                     /locus_tag="P5179_00175"
     CDS             5531..6250
                     /locus_tag="P5179_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRS93667.1"
                     /translation="MNVDLSATWQEASRVTLRGRIAVVIDVLRATSTIVTAIQSGARE
                     VIPVETPEDARLAAQETGAYGAVLGGERGGLKIEGFDFGNSPYEYTPHSVGGKTVVMT
                     TTNGTRAFAAAREADITFAAALLNIGSVANVLLHEGRDVAVICAGTEGRVSLDDLYCA
                     GALIERIVTPGCDWVLNDGARLAVHWYGSNTGTSFDVLASCAHGRRLAELGFHRDVEY
                     CSGVDTVTVVPVLKGRSLVSR"
     gene            6266..7435
                     /locus_tag="P5179_00180"
     CDS             6266..7435
                     /locus_tag="P5179_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93668.1"
                     /translation="MARANGAHGYRAVLSAPRMALNGTVIRTGTIAILVCSVILCVFT
                     YLAVLADPSIPLLFAWRKLRLFPWLADLRLNPVGTFVQTTGKAIAGLVLLYSTGAQVK
                     TLFRHAKGDVSFSAHEAWHSGRANSHALVTAIALLILVATGTLLSALALGLPGRIPVA
                     GEIFAGFGFLLALPLVFLLLVCLALIAGTLTVGVAIANTLDDDWLGTLVNVGALLWSQ
                     WWRFLVYEGIVFCVTIASWLAATGLLFTCTGLAITLVGLTTQGRLAAVAYVASQFVPG
                     LDWVLSRVVPQFDVWSIVVGPGVAVGGAFVSFGILFLLAVVTGYAAGVWSAGQMATYA
                     ELRRHQYSQDIYEKEDMIDRVAALAPTLKSGRHEAGVIAPSGERKDAGDQKPPAS"
     gene            complement(7459..8034)
                     /locus_tag="P5179_00185"
     CDS             complement(7459..8034)
                     /locus_tag="P5179_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93669.1"
                     /translation="MALQKTIRTAFLSLGVLGAAMMSGCILSNEDEAGTVKDHAGPNT
                     PVTVENAQTVFNMVFGTIKGAMEEPLSKPSAAASETRTINGSASGKIVVKTTPTANGY
                     TEELEITDFSNDGELFIGGKLTNDMTTTEHGETGNITGDIAVAGFFKGKVGVDVMMEN
                     FGDYFMVQGMVTVGGEEVSVFFHPTYTKVAR"
     gene            complement(8176..9441)
                     /locus_tag="P5179_00190"
     CDS             complement(8176..9441)
                     /locus_tag="P5179_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRS93670.1"
                     /translation="MPSRNSVLSGVFVLGVLLLAGCDEITRSGDPGGATGAADPRLAK
                     EAVPPALVDANMRFAFGIYQRLVETPPAENLFISPASISLALAMAYNGAAGNTKEAMA
                     RTLGLGEMTLEEANQANLVLLSNLVYADETAKLAIANSVWANQGYGFYESFLDRNRRY
                     FGAEVATLDFARPSALATINGWVERNTFGKIPSILDEIPDFAIMYLINAIYFKGEWTN
                     KFDVAETRMRPFTLSSGSAKNVPMMTRHGRYAYLQNELFQGVALPYGNKRFSMYVLLP
                     KEDVGLAALNRALTVSNWTTWVAEFDTMEGTISLPRFEARYEETLNSVLSAMGMGVAF
                     DPYGADFSAMLSVSPSRNVFINEVKHKTYLKVDEEGSEAAAATSVGFGVTSVPVTFNM
                     VVNRPFFLVIRENKSGTILFMGTIVDPSS"
     gene            complement(9473..12202)
                     /gene="polA"
                     /locus_tag="P5179_00195"
     CDS             complement(9473..12202)
                     /gene="polA"
                     /locus_tag="P5179_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRS93671.1"
                     /translation="MSDAKRLYLLDGTALVYRAYFAFIRNPLINSKGENTSAPFGFVS
                     ALLKIVREEAPDYLAVVFDSAQPTFRHERFPAYKATRERMPEELVAQLPRIDQIVEAF
                     GVPIVRLDGFEADDLIGTLAVQGQNAGLDVTIVTGDKDLMQLVNDRVRILAPGKTKNE
                     WERIGPEEVVARWGVSPEQIRDLLGLMGDSSDNIPGVPKVGEKTAVELLKKYGSLDAV
                     LEAAPEIQKPMVRQNLMDFALQARLSKELATIAVDAPVTLDLEAFTWVAPDPARVVPV
                     FRELEFNQLIEQLGVQAPGPANKLPLEFRTVTADMLPGLCEQIRAAGAFSFDTETTST
                     DPRSASLVGISLACDGLTGYYLPIGHRNREPDGLAFTRSAESPVNLPLTTVKEHLQPL
                     FADTMLLKIGQNTKFDLAILNRAGFSVDAPLFDTMIASYILDPSARHGLDAMAEEHLG
                     HRMIPITDLIGKGKDEITFDLTPVDKATEYSGEDAVITYRLGRLFEERLKTNGLDELF
                     RTLEMPLVPVLLQMEETGIRVDAEALRDLSREISQQLHELEEQVYDAAGEKFNINSTQ
                     QLAHILFDVIGLPARRKTKTGYSTDVDVLEALAPLHPLPKLVLQYRSLAKLLSTYVDA
                     LPKLVNKKTGRIHTSFNQTVTATGRLSSTDPNLQNIPVRTELGGQVRRAFVPEKGWLM
                     LSADYSQIELRIMAHLSEDTTLIKAFQNDEDIHTLTASLVFGVPPAQVNRELRAQAKT
                     INFGVIYGQTPFGLSQQLGIPQREAALFIENYFKTYPGVKKYGELTIERARKDGFVST
                     MLGRRRFVPDIDHQDVNRRRFAERTAINTPIQGTAADMIKLAMIRIHERIAAEKLRAR
                     MLLQVHDELLFEVPPDECDALKDLVRAEMSGALELRVPVRVDIGVGENWLVAH"
     gene            complement(12263..13333)
                     /locus_tag="P5179_00200"
     CDS             complement(12263..13333)
                     /locus_tag="P5179_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93672.1"
                     /translation="MDCAPVVIPYHAIFLRDHWEEVTTEPWWKTSDGNIASSVQVERD
                     FLESTGMDWTGVGIGASRQFRETHAASVRDGVPYLTNTVTGEETELRHWPRSGDHYSV
                     NCPSVTNLEDIERVVQVRSAREILDAGFLDGARAVVESVGASHAVYAAVNHPSWTYTC
                     FGFENMMANMVENPALVHALSERMCKKAIEEIKAFAAIGVDFVWVELCLVGRDSISLS
                     AFREFVTPYSRPLVDVARENGMITVFYVCGDVSDRLEDLVSVSPMCLSFEESKKGFCV
                     DLDTISRVVGDSVCLFGNLDAVDVLENGSDALLAREIERLLEIGNRTGRFVMSLGSPV
                     TPDTPVARVRRFGELTRQFSSR"
     gene            complement(13391..14236)
                     /locus_tag="P5179_00205"
     CDS             complement(13391..14236)
                     /locus_tag="P5179_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRS93673.1"
                     /translation="MIKTSTYPCKMIAKDIPGAEGPCVNMRGEFFMVAPNLGQVLRVN
                     ESGETSIFAETGGIPAGLQCDRSNRLWLADMKLGILSISPEGIVRVETPEFDGKPMRG
                     CNDCAFDSRGNLYVTAPAGSGKDAPNGEVFCRKNNGELVLLDGGFQFCNGIAVNADDR
                     ILIVAETLTRWLWAYDILAPGKVANKRKWAQLVDVEGGLGPDGLDFDSAGNLLAAYWG
                     GGSLDVFNPAGELIERIMLPFKAPSNLHFGGRGSRTIYVTEHDSNGLWKLEWERDGQP
                     QFCER"
     gene            14330..16378
                     /locus_tag="P5179_00210"
     CDS             14330..16378
                     /locus_tag="P5179_00210"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRS93674.1"
                     /translation="MILQNDLIRVELGDETGGFRSLKDLRLDREWVCAPDRSLLFRLM
                     VPDGERECEHVDSDVPAVVPGGSSCVLTFARNGISMQAFLELDGDALVCRLQIVNDGP
                     APVEEIMFPYVRGLAPVEKACITWPHFHFRRIDDVFGRGLGGDHHSWNEMAQKYVGRY
                     PHWLTTAWCDYGNDSSGIALEGRHKDFSIMDFCLHKVVEKHFDPVRRSLDLYTVHPHR
                     VAPGETFTTPPVRIRLHEGDWHTVADEHRGWLDTWIGKPERPAQFAESIGWHFYFMKH
                     QDGLEVNSYADLPRMAKSALAAGCRYLMVFGWQTGGHDNNYMYRYVPNDSWGGEAALK
                     AALEECRQLGVQVIPFFNGTLANTQMPEHREYGYRWEATTRAGHPYYAGDWARNNFDA
                     PSRNRSMLHHEVCFCEEYRPHFLDAVTRIVKDYGFGNTQLDQIAEKMFVCYNPSHGHG
                     APDRAFVDGLAALLPGVRKIVRDANPDGVIVGEGFNDFVNQWCDSSWTWRGLFDFPEA
                     VLFSLPWLFASCEIDALEYGDANRAFAYKLQLDMKIDGGDGNISDYPEFCSFIKSHAE
                     LRSRVAPYYVYADFRDEENLVIRKPSGVFAKTYLNRNEGKVGVVIAETAGKPADVEFA
                     HTWPTDERAARVWSSQGEMSQITVEGVVRMELRPFEVLVVCLDGVRTRHLPNVRKDLS
                     "
     gene            16380..17264
                     /gene="pheA"
                     /locus_tag="P5179_00215"
     CDS             16380..17264
                     /gene="pheA"
                     /locus_tag="P5179_00215"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRS93675.1"
                     /translation="MVVVAFQGELGAFSEAAARAYFGNGIQLSPSATFRDVFLKVSTG
                     EADRGVIPIENSLAGSVHENYDLLLEHSLHIVGEVKLRIVHHLVANKGVALPDVRRVF
                     SHPQALAQCQEFLRGLPGVTPVPVYDTAGAAKELKASGMRDAAAISSAQAAMDYELEI
                     LASGIESNHQNFTRFLIVALQPDADRDRPGNKKVSVAFALHHAPGTLFKSIGVFALRD
                     IDLLKIESRPIPGTPWQYLFYLDFAGDVEEPRVGKALDHLGELTSFLRVLGCYPIGDT
                     IDSATPFARDRAGQDSER"
     gene            17261..17821
                     /locus_tag="P5179_00220"
     CDS             17261..17821
                     /locus_tag="P5179_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93676.1"
                     /translation="MTWLERVGAFLAHRRVQSMAAEPVEIREALRAGGKTLVFLPQEP
                     ELLDSGVSICVTLAGWFGPLALCCVSRDDKLPDLSNSGYPAVLFPWAISRMGLPTRNV
                     REKVKVLAPTIAVDLHPQFNLPTAYLATESGASLRIGFGDAPSGYFNLRYACPSFDPA
                     NSALHYGRFLRTIENLRADTFEEPPR"
     gene            17818..20700
                     /locus_tag="P5179_00225"
     CDS             17818..20700
                     /locus_tag="P5179_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRS93677.1"
                     /translation="MSILESFVALDFETTGIDPFSSEIIEVAALRVQSGQIVDSFQTL
                     VQPHKMPSVSIQRLTGITADELQGQPSLEGVMARLRRFVGATPLISHNADFEVSRLRM
                     SIPAAELPTVFDTVELARIALPRLPNHRLETLVDAFSIENPEQHRALGDAQTLVEVFR
                     ALVNTMRSFSPVRLDTLAKLARSLPRPEPPFGAIADLLLDIQAAAARSAFDQVSPPPF
                     RVGALTSLPNTFGAFDDNRLEPATSLEDIDAESIAETLGAHGDFARAMPTYEVRRSQI
                     DMARAVTEILNEGGTLLCEAGTGTGKSLAYLVPSVTWALKNSHRVVISTHTKNLQEQL
                     FYKDLPLVAGTLDVPVRSVVLKGKNNYVCLHQWHEEIGLPPAEAVDASRALLLPLVCW
                     IDETESGDLEENGAFQTLLRYVPGAYSLAVRLSSEGKPCGRRTCRYYDRCFVTRVRRA
                     AQQAHIIVVNHSLLFSDLAADHGVIGEYENLVVDEAHTFEETATDYLGIEFSLPLVRS
                     VLDEIITPHARSLLTRIRGNLRRSKIEDSTRKSFEVQLGATGQAAELAGEKAEAFFRA
                     AKNELGAPHPSSIYSSKMRYSSEVNVFNEIREHRDDLSFALKTLRDGLQTLALWLRDL
                     SSGGIPEQDELLADTELRQADVARLTAAMDLITRAEDEGMVYWSEMPPHGDETRLKFI
                     AAPLDVGATLRRDFYPSLRSLVMTSATLSVAEKFNYFRQRLGLEDDSVRTMAVKSPFD
                     FENQVHIALPGWFPEPRDSAFEPEVISLLRVLIPRIHRGTLVLFTSHGMLENAYNALK
                     QPFEEQGILLLGQRFDGSRSSITNRFRQDRQSVLFGTESFWQGIDVPGEALELLIIVK
                     LPFSVPGEPLETAQSEALAAAGKNPFLHLAVPKAAIRFRQGFGRLIRHRNDKGAVLIL
                     DTRLVSKDYGRAFHRSLPASYTVYRSQEELIAGFEAWFAAEITTKL"
     gene            20830..21813
                     /locus_tag="P5179_00230"
     CDS             20830..21813
                     /locus_tag="P5179_00230"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRS93678.1"
                     /translation="MVPVRVAVTGAAGQIGYSLLFRIASGEVFGKGLPVVLQLLEIPQ
                     AMKALEGVAMELEDCAFASLAGVELSDTPEQAFSGANWALLVGSKPRGPGMERIDLLR
                     DNGGIFVGQGKALLNAASDVRVVVVGNPCNTNCLIAMAHAGDIPPERFSAMTRLDQNR
                     ATAQLARRAGVPVGQVTNVTIWGNHSATQYPDFEHARINGDPAETVIRDRPWLENDFT
                     ETVQKRGASVIAARGASSAASAANAIIDHVKSFLLPTPGGHWFSAAVKSDGSYGVPAG
                     LVSSFPITKKETDNWCIVPDLILSEYGKRMLANTVRELESERDMVKDMLGS"
     gene            21861..22877
                     /locus_tag="P5179_00235"
     CDS             21861..22877
                     /locus_tag="P5179_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS93679.1"
                     /translation="MAILLMKDWVEERLKTPPRNSCAATTPAEFLEWQRSFRRSIKRA
                     LGRFPESVPLAPKTLEKTDCGTYTREKVVFHSEPAMAVVAWVLTPRRTKRGQRVPGLV
                     CAHGHGLGKNPLVGLDNNGQVHEDYQHRHAIQFAERGYVTIAPDWRGFGERRDGPPWD
                     DKGRDGCNLRHLAAGYFGFSLLALQIHDGMRCIDYLQSRKEVDPSRIGCVGVSFGGTM
                     TTYLAAMDRRVKAAVISCYLSTIVNGLRRTNFCGAQYMPELRAHGDIPDVALLAAPRP
                     LLAEVGEKDRCFEVDDALQACERVRAGYVAAGHPDRFDVDLFPGEHEFSGRKAFDWFD
                     RWLK"
     gene            complement(22885..24015)
                     /locus_tag="P5179_00240"
     CDS             complement(22885..24015)
                     /locus_tag="P5179_00240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS93680.1"
                     /translation="MLAISIIAFVLALGYVVSAGILAGALRGNRRVRVPASAANTSVT
                     VVIAARDEEEALSVCLESLSRQDYPPDAWNLVVVDDRSQDGTGQVVRRARDENDRIHA
                     LRVDECDPAYGGKQNALAAGIEHMAGMGILGDIILFTDADCVVPSSWIADLVAAFSSP
                     RVGMVAGWTTIPTTRSISSLLEKCELAYLLSVAFGMIALGRPVSAIGNNLGIRRKTYE
                     DVGGYRNLGYTITEDCALVQRVAATPWSITTARDTAAVLTRPCSSIGDFVRQRIRWAA
                     GVRHLQVGQMLLPAFAFVHRSWIALTSAMAWCGVLDWRIPAMVWCSSFLADAALIGAR
                     GNWKWAPMSPLLTLWQVIYQPVVAIMVLLSGRTRWRGSRYRL"
     gene            complement(24074..25081)
                     /locus_tag="P5179_00245"
     CDS             complement(24074..25081)
                     /locus_tag="P5179_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS93681.1"
                     /translation="MPVSSMKSLPLRTYLRAASAIGENIRKKEAINPLYASYKVTNRC
                     NFRCKFCNVWFEKTPVLETELVYRVLDNLERAGVFLLSLEGGEPLLRNDIGDILKYAG
                     AKDYYILFTTSDRSLIEQPMVEYCRNIDFLHISIDEGHDNLDMFSLLPEAVRWGSIVC
                     VQVVVMKENLPALEDKVARCRDAGAKCVIMPAVELNRTRNHFPDLDAFQRECLRLKRK
                     YPRTIISPDPYFAALREPHGCSTGSIIVDCDGGLFYPCRTLESKPVNLLDVELRDWLH
                     GEEAKAFRRQMATCDRRCGWYQYYAVSAFTSPVHVWTSISPYVWNVLAPRKKQNHNGR
                     K"
     gene            25473..26945
                     /locus_tag="P5179_00250"
     CDS             25473..26945
                     /locus_tag="P5179_00250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRS93682.1"
                     /translation="MSERGAVLIVGDDQATCETLSDFLTSAHHTVHVVTSAPEAITEL
                     QNRPYDIVVLDALMAGETGLDLVARIKAVDFRLQVIALMDIGAGEAAVRAISQGASSY
                     LLRPVRLAALLNRVATALHTRSFFRSLQEPVSDPERRPDLVRGLEIAEKLFQFDRELV
                     SILDYHLVVESVLRSALSIAEADMTAFLMIRDRVVSIVVNVKEDSPLASRQAVFERMT
                     SKWDSWGGNSLHLDDVLWSGLEEDALGSTREASVYPIVVQDVLVGAIGAFATGRRSLD
                     PRADVLVPIIASRAEVVIENAFLHEHTKILATTDPLTGLLNRRVFREGIVREFERSRR
                     LFLNKRPGGDLSVIMLDVDHFKKFNDTYGHQLGDKVLKMVATVLLNVARRATDMVARY
                     GGEEFVVIAPDTTMENARIVAERIRETLQESVIESEAGPLRVTASFGVASYPRCGAET
                     VEELIEQADEALYAAKRKGRNRVEEAASRTESSTPPTQPQ"
     gene            26956..27876
                     /gene="ftsY"
                     /locus_tag="P5179_00255"
     CDS             26956..27876
                     /gene="ftsY"
                     /locus_tag="P5179_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRS93683.1"
                     /translation="MISSIWSRLRHGLSRTRSTLTLRLRSILGGALTAETLETLEEVL
                     ITADVGVMTAAKILKRLETDAASSGGDPAWAMRSVRTTVEEILRAAHRPFALPEEKPA
                     VVLVVGVNGVGKTTTIGKLAARYAEQKRRVLIGAADTFRAAADEQLLVWAQRARAEIV
                     RQQHGADAASVAYDAVAAAQHRSADLVLIDTAGRLHTKSNLMEELRKIKRVLGKLSPT
                     FPHHVLLVLDATTGQNALVQARQFHEAIGVTGIALTKLDSSAHGGIVIAISDELRVPI
                     QFVGVGETLDDLQDFDPHAFTEALFDIGQD"
     gene            complement(27959..28639)
                     /locus_tag="P5179_00260"
     CDS             complement(27959..28639)
                     /locus_tag="P5179_00260"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS93684.1"
                     /translation="MNLTNHNQKNDATNDMRCIMWKTMALLAAVILGMAYTTQAAEQT
                     ALQALKQRQRAIEQAQAALARAPRDTARQAALRDAITEAFDFDRLARESVGRHWNAMT
                     PEQQRDYLTVFRSLVQRSTVRKLQRYRAAGTTYSAPTGNAQNTVITTIVTSTSGEKVA
                     IQYKMHKVNGRWWVWDTTIGLDTEISDYDVSTAENYRSAFNRIIRDEGIPGLIAKLRE
                     KERKGSDL"
     gene            complement(28608..30110)
                     /locus_tag="P5179_00265"
     CDS             complement(28608..30110)
                     /locus_tag="P5179_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS93685.1"
                     /translation="MNALVKYLLAFVLIFCGFQIASAEAPPTDTLRLTLRECLDRAMQ
                     GNLDIREAQLERDRALIDQLEANRAFWLPVFEARSYLSVVKDARGYVTDHPLTTSWDN
                     FGPCFEFHLQAAQPISTLGRVNALKRAARNGIAAREAGVEVRRASVAAEVYRLYYGVL
                     VARELLEILDDVSEKIAQARTRVREMLEEDSDRVTTTDLMRIDVYTYELERKHIEANK
                     SVELALGALRRALGIPYDAHFDVVTDRLRPIPETLPSLDSLRAYAFQNRPELAQVNAA
                     VEARRQQLIAARAERYPTPFVGVEVGLRAAPGRELLTENPFVGDAYNGRSVKAALGLQ
                     YTLDNRSREAGIQRAEVEYREMLRKREWAEAGIALEVQKAYLEADEARRNSELGRRSL
                     RAGSSLMIQTLERYDMGVASTRDLLEAYGAYAKSQGDYYQTLYSHYLALGEVYRTIGK
                     PLSEITNGTNGVGHSELEGEGDGDDAQFEEPVEPENSGENGEPYEPQPEE"
     gene            complement(30275..30640)
                     /locus_tag="P5179_00270"
     CDS             complement(30275..30640)
                     /locus_tag="P5179_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS93686.1"
                     /translation="MAKAISLAPVIIAIILAAFGQVTMKYAMFGAPLPVTSPVEAARN
                     ILSRPMVYVGLVFYAGSSFLWLISLSRLDLSYMYPFTALLIVIITFVSALVFNEPITL
                     WKVVGVFLICAGLLSIARS"
     gene            complement(30944..33808)
                     /locus_tag="P5179_00275"
     CDS             complement(30944..33808)
                     /locus_tag="P5179_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS93687.1"
                     /translation="MAKQTDMYDPTLKDQSEYDHKEIYIISNTHWDREWVYPSAETRL
                     LMLEFMDNMLDLLDKQPDYHSFTMDSQTLCIQDYLDFRPERREQIERHVKSGRLIIGP
                     WCSLPEEYIVNGESLVRNLVIGHKVANSLGKVSKVGYTPFSYGQTSQMPQIYRGFDID
                     TIIFYRGIKTEKSEFIMRGPDGSELLGMRFGALSRFSYFFYLFRMVAYNMSRDEAKYD
                     WFRGSLPFKLAGDHHVNAHYYICDPDATGWHTEHIDTGARKLLRDESQHFSTRYIASM
                     QGFDSSEPDPRELDLIKETQKHLPNHVIKQSDLAEFMDRLKEEVAAGRLKPEVIEGES
                     RNPNSVGKWTHLFGDVISSRVRIKLMNNANEQNVQRKAEPFCALAMTLGAKYPTTVLD
                     ECWDNIMKNHPHDTICGAGVDQMEKDMIARGEQVNIFSEGLYRRALQKIQTRIDNSDV
                     AANEIALTVFNPSPFPRSEVVSVLLDLPDVCKYEDFTIRDEKGNKVEAQYAEFYPWGT
                     LVRNLRDVSLQLHAERVRVHFEALDVPPFGYRTYTVKHEEDVVPKPMKTLVTAVNTME
                     NEHLKVKINANGTLQITNKETGDVFDNMHYLEDGGENGHSWTTIPPDHDEIITSHGAN
                     VYIAQMESGPALARFRVEYHMMIPEALDFETMEAYTGQTPRAQIRHPRISKRSERRKE
                     MVIRSEFTLRKGSKVLEVYTKVNNECENHRLRVCFPTGIQAATSDSEASFDVIQRQVI
                     RTPDNWYWGRPNPVLPMHRFVSFSNGKSGITFLNNGIREYEAMEDADRTVALTLFRAF
                     TFRNPPILDTYDVYPEMKLSQCPGEMEFRYNIYPHKGDWAAAEVYKEADHINLPLEVA
                     EVGPHKGDLPKSMSFLSISPANLVLSAFKKAEERDTFILRVFNPTGDAMNGTITFYRP
                     IKKAWLCNMNEERRQDLEPKGNVLTLDVPKKKIMTIEFEA"
     gene            complement(33956..34981)
                     /locus_tag="P5179_00280"
     CDS             complement(33956..34981)
                     /locus_tag="P5179_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS93688.1"
                     /translation="MKRPFVLFCTALLLVVSGCLGTIPLGERGRAAHVLRFDDNGVFT
                     IVQFTDTHLGSRDAGLRDDSTYALLDSIIRWENPDLIVLTGDIVRFAWEPQAVPSWRR
                     LIAFFDSTGVPWAFVHGNHDAEVRGYAAVDSLIATARNCLYTSGPDSLNQPGNYVLPV
                     LSRKGRGVSALLWCMNSGAGSSEPSGYGWIRANQVKWFRATSERLKTQTQPNLTQLAF
                     FHIPFAQYQTVWDARTCTGYKNEKVCMQGKDEGAFAAFVEYRVNGCFVGHDHTNDYEG
                     TLEDVTLCYGRASGYRGYGKEGFLRGARVIKIKDGVRGFTSHIRLADGSLAERPVHTP
                     GTALPTP"
     gene            complement(35061..>35757)
                     /locus_tag="P5179_00285"
     CDS             complement(35061..>35757)
                     /locus_tag="P5179_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS93689.1"
                     /translation="TVRDLGFDGIEWTAYYFPETTKEYLLHIKRETFVRGLDISATAV
                     GGNFIQPTREERKKQIANVKSWIEKSAWMGSTALRVFAGYVTEGMAREDADQMVRDGL
                     AECARLAEKEGVILALENHGGVTADAEGTLRLVKPLNNPWVKLNLDFGNFTGDIYAQY
                     EACAPYAVTTHTKVTVRQGEAREKVDYRKVVRIMRNAGYRGYINIEFEEPEDPIVGVD
                     RFAAYLRGCMEDA"
BASE COUNT         7337 a        10306 c        10424 g         7690 t
ORIGIN      
        1 ctgggagact acccagcggc agaatcggct gttcttccgc ttactcagga aacaggagcc
       61 ggcgcgccca attctgcgtt cgccgcgcat ggacggttcc tgctcgggct ggtctgctgg
      121 cggacggggc gctatgcgga tgccattcga aacctccacg cagtttccgg ttcaccagtc
      181 gtctcagaga caacaaaacg ggacgcgcgg cttctgagtc tggcttccgt cgcgcgggtc
      241 gagcaagtca acttcgcgga gtttgatacg tcccaggccg cgttcgttcg cgacgccctc
      301 caactcaacc gtgcgatcgc cctgttcaac gcatcccaga tgactgccgc gcgtctggtc
      361 ctggagcaac tgcagaggat ttctcaggac actcctttcg ccagtgaagt cgccgcgctg
      421 ctggcggacg tcaaaagcta cgagcgaacg gaaatccgac tggctgtggt tacgccgctc
      481 tcaggggagg attctacatc cggcgcagcg cttcttgacg gagtgaagtt tgcctttgag
      541 cgactcgcgc cgcccggtct ttcgcccctc gtgaccaaga gcgcagcgac gcaagtggag
      601 accatacacg ccgtggaggg ttttgcgcgt gacacaacgg tgctggcggt gattggtcct
      661 ctcacgacgg agaactccat cgtcgcgggt gcggtggcta acgcgatggg cctccccctc
      721 gtcacgccga cggcttccgg ggagggtgtt tgtgggattg gtgataacat ctttcagatc
      781 aatctgacgg cgcgcgcgca aggggcattt ctcgcgtcgg tggttgcgga cagtctccgg
      841 gcgcaggctg ttgccattct cagtacgtac gacccgcacg atcaggctct ttcctcggaa
      901 ttcgcccgcg tggttcgcga gaaaggtctg attgttattg cgcacgagta cttcattacg
      961 ggtactaccg atcttacccg tccgctgagc gcgatccgcc gggcggcgtt acagcggact
     1021 cctccccccg ccgacatgga cacggtgcac ttcatgccgg aaatcaactc gattgacgcc
     1081 atgctggtct gttcttcgca cgaaacggac ctggtcgcag tagcggcgca gattcccacg
     1141 cagaagatct ggacgcgcgt cgtgggaaac gccacgtggg gcaattcctc cgtgcgccag
     1201 caggcgggtg aggccgcgga gggggtcctg ttcgcaacga cgtttgacca gtcgtatccg
     1261 gaagctcgga atttcgcaga ggcgtaccgc gcggaacgag gaaatgagcc gatacttgca
     1321 acggcgcttg gatacgacgc cacgtcgctt gtgctgcagg caatatctac cggcgcgagg
     1381 acccggcccg aggttcgtcg tttccttgca gacgtgaaag aatggcccgg cgttacggga
     1441 ctcatcaccc tggcgccgga cgggtgcaac cggaacgcca tggtccgaat gattcgcggc
     1501 gggacgattc ggcccgtggg ggactggcag atcctttccc gagcggcgac acagaatgcc
     1561 acgcctcggg ttggccaata aaggagcgaa gcctgatgac cctggaagag aaacatgcac
     1621 ggctcaggga aatgctcctc gaggccgcgc cggttgtggt agccttttcc ggcggcgtgg
     1681 acagtacgtt cctccttgcg gccgcgcaca gagcgcttgg caaggcgaaa tgcatcgggg
     1741 taactgcggt gtctgaatcg ctggctccag cggagctgga cgaagcacgg cagatcgcga
     1801 accagatcgg agccgaatgg acgacgatcc agacgagaga gatggacaac cctcgttacc
     1861 gcgccaacac caacgagcgg tgctattttt gcaagacgga gctgttcagc actctgcaga
     1921 ccctcgcgcg cgaacgaggt tttgcgactg ttcttgacgg gttcaacgct gacgacaccg
     1981 gggactggag gccaggtgcg cgcgcggggg tggaacaagg agtcaagagc ccgctcaagg
     2041 atgcaggcct cacgaaacag gacatccgcg atctctcgcg gtcttacggt ctcccaacct
     2101 ggaacaaacc atcgtttgcg tgtcttgcct cccgcctgcc gtacaacacg gaaatcaccc
     2161 cggagaggct ttccctcatt gcttccgcag aagcagttct gcgctcacac ggttttcgag
     2221 cgtttcgggc gcggtaccat ggggagactg cgcggcttga gataggaatt gacgagatac
     2281 cacgattcca agaagctatg cttgcgcaat ccgttcgaga tgccgtcatg aaggcaggtt
     2341 ttgctggtgt gactcttgac ctcgcgggtt tccggtccgg aagccagaac gagacaggcg
     2401 gcagtggcat tgtcccgctt ccgtctgctg cccgcgatgg gctttcgatc atcaagaaac
     2461 aggggtttgc cgttgcgaag acacgtatcg aagggaacat gatacgtatc atcgtttcgt
     2521 gggaggactt cccccgaatt ctcaaagagg agactcgcat ccccctcgtg gctgcgctgc
     2581 gcggacaggg gtacgtgtac gtcgcagcag acatccctta ggtattcgtc accgaaacgc
     2641 tccgcgtccc gaagccacct gccggtgagg aggaaggtgt gaggtggttc gccgtgagtc
     2701 tgctctgcct gttgttccat ctgcaggcga cagccggtga tgaattcgaa ccgtccgacc
     2761 ctgacgaagc ttcgtatcag aacgcccggg cggagtacgc ggaactcgat tcggcaactt
     2821 tggggaggga tcgatcctct actgacaggc cgttcttcgg tgcgttgcgc aacgcgaggc
     2881 atccccgcct ggcttttcgg tcccgcctga caggttggga cctggccgca agtacatctc
     2941 ctgacaactt caccttcaac aacaggatta tggtgcggca gggaggtgtt gtcgccgggt
     3001 tcatcaccga gagggatcac gacgaaccaa ggatgatgga ttaccgaagc ggaacactcg
     3061 gtttcttatt ccacggagat tccacgcgcg tcgtactcgg tgccatggcg gcacacgtcg
     3121 gctcagggct gatcgtcggc agacagggac tcaactggac aggcgcgctc tcgttccgtg
     3181 aacgcgaaac cgcactcact cccgccctga ctaccacaga atccggagca ctccacggaa
     3241 tcggctttcg ctacgttggc cgtgtatggg aaatcctcgc cctgtacaca acgccgaggt
     3301 gggacgcgac tatcgacagc gcaacgaaca gcgccggcgc aatcagtgtc acaggtctgc
     3361 accggacgga cgcagaattg cgtgccgcgg gagcactcaa ggaaacgttc cgtctcttac
     3421 gcatcggggg caatttcggg cggtcggcgc gcgtgggact gacagcagcc aaaagcgagt
     3481 tcagcccccg cacgctgtat cgccattcct cgaaagcaac cgacgagatc tctttcctcg
     3541 gtgtcgacgc tcagtcaaca atcgggagtt tcggcgtccg gggtgaagcg gtcctgcagg
     3601 atgagcgcga atacgggggc gccggtgcgc ttcgttttca caacgagtcg ttaaccatga
     3661 ctgccgccgg ttggttgtac tcgtcggcgt ttacctcgtg gcatggaacg ggatggagcc
     3721 tccacagcga ggcttcctca gagcgtggtg gtgcactcgt ggttgaatgg gctcccgggt
     3781 cgggcgtcca cgtggacgga ttcctcgcgc attccgtgga gccgcttgat gcggcaggcc
     3841 gcgattttcc tcggtggaca actgtcgagg aacttcgctg gcgaacccct ctttcaacgc
     3901 gattttcgtg gatcggaagg tggaaacaga agtcgacgcg gtctactgtg gcagagggga
     3961 tattcatgga gcgaaggcgg gacatctctt ctgatcttga gctgatcgct tcagggatga
     4021 ctttccgagg cgggtgggcg tttgcccaaa atcaatcagg agaccgggga ggacttgtgc
     4081 ggatacggat gcaaagtcga ccggcgcgcg gactggactg cactctgggc ggcgccttcc
     4141 accgcacaaa ctcgacctcg ttgccgttgt acttctatga gcaacaggtg ccgaactacg
     4201 gttttctctg gagcctttac ggatcccgcg caagctggac ggttcgtgcc cggtaccaca
     4261 cgggcagggc caccgcatcg ctgttcgtac tgggtacggg gaccttgaac gaagcggcag
     4321 accggctgac tgtgactgcg cagattgatt atgttctacg atgatcgaaa ttgttcggga
     4381 tgcgtgaatg cgctttttgg atgaggaaca tggtgacgac ttctcccgtg cccctgttgc
     4441 gaacacccct tttcaaccgc cacctcgcac tcggcgcaag gatggctccg ttcgccggat
     4501 tcgaaatgcc gatcatgtat cggagtatcg cggaggaaca caatgcagtc cggcacggct
     4561 gtggcatatt tgacgtgtcc cacatgggca ggctgcgttt cagtggaacg gcgggacacg
     4621 aacttctcga gcgcctgaca actatccacg ttgcttccgt aggtgccggc accgcgcggt
     4681 acgggttcat actgaccgac agcggcggga tccttgacga tgtgattctt ttcgccacgc
     4741 gcgcagcgga gtgggtcctt gtcgtgaacg caggaaaccg gcgttccgtg gttgagcata
     4801 tggggaagct ggaaccccat ccccgttttt tcgacgatac cgaatcaacg ggcatggtgg
     4861 cagttcaggg gccagcaaca cgggcggttc tgaggcaggt aatgccgggg cttgcggtac
     4921 ctgaccgcgc gaaccgtgtg attgaaacgg attggaacgg cacgcccgtt gtcctctcaa
     4981 caaccgggta cacaggcgaa gacggagtcg agatcatcgc ggcgagcggg cgtattgagg
     5041 ccctgtggga ctccgtgatc gaagccggag ctgtgccggc tgggcttggc gcgcgtgata
     5101 cgctgcgtct cgagatggga tacccgctcc acggccatga actttcgaag gaaactgacc
     5161 cgtggcaggc aggtctgggg tgggccattg accggaggaa cgagaaattt acgggccgat
     5221 ccgcggtgct ggcacgtgag aatgacaaga caccccgcct agtgggtctc aaggcggcag
     5281 gacgcggcat acctcgcgcg gggaatctgg tggaagacca cggaattgta gtgggggaag
     5341 tcacctctgg gaccttttcg ccttcgctgc agcagggcat tgcgctggcg tacgtcggct
     5401 tcggcgcggc aagtccggga aaacagtttt cggtccgcga ccgcgaatcc gaatcaagac
     5461 ggatcacgat ggaacaggtt ctcgcgccgt tttaccggac tggcacccgc agaagcgcca
     5521 ggaacgcatc atgaacgtcg atctttcggc aacctggcag gaagcatcca gggttacgct
     5581 gcgcggtcgt atcgctgtgg tgattgatgt cctgcgcgcg acgagcacca ttgtcaccgc
     5641 gattcaatcc ggcgcccgcg aggtgatccc ggtggaaact cccgaagatg cgcggttggc
     5701 agcacaagaa acaggagcat acggagctgt actggggggc gaacgcgggg gcctgaagat
     5761 cgaagggttt gatttcggca attcgcctta cgagtacacg ccgcattccg tcggcggcaa
     5821 gaccgtcgtc atgactacga cgaacggcac gcgagctttc gccgcggccc gggaagcaga
     5881 tattacgttt gcggcggcct tgctgaatat cggaagcgtc gcaaatgtcc tgctgcacga
     5941 aggacgtgac gtggctgtta tttgcgcggg tacggaaggg agggtatccc tggacgacct
     6001 ttactgcgcg ggcgcgctta tcgaaaggat cgtcaccccg gggtgcgatt gggtgctgaa
     6061 tgacggcgcg aggctggccg ttcactggta tggttccaat accggcacca gctttgatgt
     6121 ccttgcctcc tgcgcgcatg gacgacgact ggcggagctc ggattccatc gcgatgtgga
     6181 gtactgctcc ggtgtagaca cggtgacggt agtacccgtg ctgaagggtc gctccctggt
     6241 cagccgatga atgaggaggg cgtgattggc acgtgcaaat ggggcccacg gctatcgtgc
     6301 cgtactctcg gcaccgagaa tggcactgaa cggcacagtc atccgaacag gaacgattgc
     6361 tatcctcgtc tgttcggtga ttctctgtgt tttcacgtat ctcgcggtgc tcgccgaccc
     6421 gtccatacct ctcctgtttg cgtggcgcaa gttacgcctg tttccctggt tggcggatct
     6481 gcggctgaac ccggtgggaa cattcgtgca gacgacgggg aaggcaattg cagggcttgt
     6541 tctgctgtat tctaccggcg cgcaggtaaa aacccttttc cgccacgcga aaggcgacgt
     6601 ttcgttttcg gcgcatgaag cgtggcattc cggtcgagcg aacagccacg cgctcgtgac
     6661 tgcaatcgcg cttctcatac tggttgccac cggaacactg ctgagcgctc tggctcttgg
     6721 actgccgggg agaatacccg tggcggggga gattttcgcg ggtttcgggt ttctgctggc
     6781 gttgcctctc gtattcctcc tgctggtctg cctggcgctt attgctggca ccctcaccgt
     6841 cggcgtcgcg atcgccaata cgctggatga tgactggctg ggaacgctgg ttaacgtggg
     6901 cgcgttgctg tggtcacaat ggtggcggtt cctcgtatac gagggaatcg ttttctgtgt
     6961 caccatcgcc tcgtggcttg ccgctacggg cctcctgttc acctgtacag gactggcgat
     7021 aaccttggtc ggattgacaa cccagggcag gctcgcggca gtggcatacg tcgcgtcgca
     7081 atttgtccca gggctggatt gggtgctgtc gcgagtcgtc ccgcagttcg acgtctggag
     7141 catcgttgtc ggccccggtg tcgccgtcgg gggagcattc gtctcgttcg gaatcctttt
     7201 ccttctggca gtcgtaacgg ggtacgccgc cggcgtatgg agcgcgggac agatggcaac
     7261 gtatgctgaa ctgcgccgtc atcagtattc gcaggatatt tacgagaaag aggacatgat
     7321 cgacagagtg gcggcccttg cgccaacgct taagtccggc agacacgagg caggggttat
     7381 tgctccttcc ggggaacgaa aagacgccgg cgatcagaag ccgccggcgt cttgatgttc
     7441 ctcagggaac gatgtgtttc agcgggccac tttcgtgtag gtaggatgga agaacacgga
     7501 gacttcctct ccgccgaccg taaccatccc ttgaaccatg aagtagtcgc cgaaattctc
     7561 catcataacg tctactccga ctttcccttt gaaaaaaccg gccacggcga tatccccagt
     7621 gatgttgcca gtttcaccgt gctcggttgt cgtcatgtcg ttggtcaact ttccgccaat
     7681 gaacagctca ccatcgtttg agaagtcggt tatctccagc tcctcggtat agccgttggc
     7741 agtcggcgtg gttttcacaa cgattttgcc gctcgcgctc ccgttgatcg tacgcgtctc
     7801 gctggcggcc gcggacggct tgctgagagg ctcttccatc gcgcccttga tcgtcccgaa
     7861 gaccatgttg aagacggtct gtgcattctc caccgtaacg ggcgtatttg gacccgcgtg
     7921 gtctttaacc gttcctgctt cgtcttcgtt gctcaggata caacccgaca tcatcgcggc
     7981 acccagaaca cccaatgaca aaaaggccgt tcgaattgtc ttctgcaacg ccattcaact
     8041 cgctcctttg taggaatcat aagcctgagg ggcaaagccc ctcctgtcga tacaaaggta
     8101 cgtggtcgga gttggctgtg ctgtgatgca gcgtacagag gcaagacctg gatccgcgtg
     8161 atgcattccc gcgcttcatg aagacgggtc gacgattgtc cccatgaaaa ggatcgttcc
     8221 cgatttgttc tcgcggatca cgaggaaaaa cgggcgattg acgaccatgt tgaacgtgac
     8281 gggaacggac gttactccga atccgacgga ggtagcggcc gcagcttcag aaccttcctc
     8341 gtccactttg aggtaggttt tgtgtttgac ttcgttgatg aacacgttcc gggaagggct
     8401 gacggacagc atggcactga aatccgctcc gtaaggatca aacgcgacgc ccattcccat
     8461 tgccgagaga acggaattga gcgtctcctc ataccgtgcc tcgaaccgag gcagggaaat
     8521 cgtcccttcc atggtatcga attcggcgac ccaagtcgtc cagttggaaa ccgtgagagc
     8581 tctgttcaaa gctgcgaggc ctacgtcttc ttttggaagc agcacgtaca tgctgaagcg
     8641 tttgtttccg tacggcaatg cgactccctg gaacagttcg ttctggaggt aggcatacct
     8701 tccatgccgc gtcatcatgg gaacgttctt tgcggaaccg gaactcaggg tgaacggccg
     8761 catccgcgtt tccgcaacgt cgaatttgtt cgtccattca cctttgaaat agatggcgtt
     8821 gatcaggtac atgatggcga aatccggtat ctcgtccagg atgctcggaa tcttcccgaa
     8881 cgtgttcctc tccacccacc cgttgatcgt ggcaagggcc gaaggtctgg caaaatcgag
     8941 ggtggcaacc tctgctccga aatagcggcg attgcggtca aggaaactct cgtagaaacc
     9001 gtagccctgg ttcgcccaga cagaattggc aattgcgagt ttcgcagttt cgtccgcgta
     9061 cacgaggttt gagaggagca cgaggttcgc ctggttggct tcctccaatg tcatttcccc
     9121 cagccccagg gtccgcgcca ttgcctcctt cgtgtttcct gccgcgccgt tataggccat
     9181 tgcgagagcg agactgatgc tcgcaggcga tatgaagagg ttttctgcgg ggggtgtctc
     9241 taccagacgc tggtagatcc cgaacgcgaa gcgcatgttg gcgtcgacaa gtgcgggcgg
     9301 aacagcttct ttggcaagcc tggggtctgc cgcgcctgtc gctccacccg gatctccgct
     9361 ccgagtgatc tcgtcacatc cggcgaggag gagaactccc agcacaaaaa cacccgacag
     9421 gacggagttg cgcgatggca tggtagttct cccgcgttgc gttcgttccg ggtcaatgag
     9481 cgaccaacca gttctcgccc acacctatat ccacgcgtac tggcacacga agttccagag
     9541 cacccgacat ttccgcacga acgagatctt tgagcgcgtc gcactcgtcg ggcgggacct
     9601 caaacagcag ttcgtcgtgc acctgaagaa gcatgcgcgc ccggagcttc tcggcggcga
     9661 tccgctcatg aatgcgaatc atcgccagtt tgatcatgtc ggcagcggtt ccctggatgg
     9721 gggtgttgat ggcggttcgt tcggcgaacc gcctccggtt tacgtcctgg tggtcaatat
     9781 cggggacgaa acgcctgcgc ccgagcattg ttgagacgaa cccgtctttt ctcgctcgct
     9841 cgatggtcaa ttccccgtac tttttgacgc cgggatatgt cttgaagtaa ttctcgataa
     9901 acagcgcggc ttcacgctga ggaatcccaa gctgctgtga caggccaaac ggggtctgcc
     9961 cgtagattac tccgaagttg atcgttttcg cctgtgcgcg caattcgcgg ttcacctgag
    10021 ccggtggcac cccgaatacg agggaagccg tgagcgtgtg gatgtcttcg tcattctgga
    10081 acgccttgat cagtgtggtg tcttcggaga gatgcgccat gatccgcaat tcgatctgcg
    10141 agtaatcggc ggagagcatc agccatccct tttcgggtac gaatgctcgc ctcacctgtc
    10201 ctccgagttc ggttcttacc gggatgttct gcaggttggg gtcggtagaa gacagtcgac
    10261 cggtcgccgt caccgtctgg ttgaacgacg tgtgtatccg tcccgtcttt ttgttgacaa
    10321 gctttggtag agcatcaaca tacgtggaaa ggagcttggc aagcgaccgg tactggagga
    10381 cgagcttggg caacgggtgg agcggtgcca gcgcttcgag aacatccaca tcagtcgagt
    10441 agccggtttt cgtcttcctc cgtgcgggaa gtccgatcac gtcaaaaagg atatgcgcaa
    10501 gttgctgagt agaattgatg ttgaacttct cgcccgccgc gtcgtaaacc tgctcctcca
    10561 gctcatgcaa ttgttgagaa atctcccgcg agaggtcgcg cagcgcttct gcgtcgacgc
    10621 gtatgccggt ttcctccatc tggagaagga caggcacaag cggcatttcc agggtgcgga
    10681 aaagctcgtc aaggccgttt gttttcaggc gctcctcaaa aagcctgccc agacgatagg
    10741 tgattactgc atcttcaccg gagtactccg tcgccttatc aacaggtgta aggtcgaagg
    10801 tgatctcgtc ctttcccttc ccgatcaggt ctgtgatggg aatcatccgg tgcccgagat
    10861 gctcttccgc cattgcatcg aggccatgcc gtgcggacgg gtccagtatg taggacgcga
    10921 tcatcgtatc gaacaacggg gcgtcgactg aaaaaccggc gcggttcaga atggcgagat
    10981 cgaatttggt attctgcccg atcttgagga gcattgtatc ggcgaacaac ggctgcagat
    11041 gctccttcac cgtcgtcagc ggaagattga ccggtgattc ggcggaccgc gtgaaggcca
    11101 ggccgtccgg ttctctgttt cggtggccaa tgggcaggta gtacccggtc agcccgtcgc
    11161 aggcaagcga aatgccgacc agactggcgg accgaggatc agtagatgtt gtctcggtgt
    11221 cgaaggagaa ggctccggcc gcccgtattt gctcgcaaag gcccggaagc atgtccgccg
    11281 tcacggttcg gaattcgagg gggagcttat tcgcaggacc gggagcctga acaccgagct
    11341 gttcgatcaa ctgattgaat tccagttcgc gaaacaccgg tacgacgcga gccggatcgg
    11401 gtgctaccca tgtgaatgcc tccaggtcga gagtgactgg agcatcgacc gctatggtgg
    11461 caagctcttt ggaaaggcgc gcctgcaacg cgaagtccat cagattctgg cgtaccatgg
    11521 gtttttgaat ttccggcgcg gcttcaagca ccgcatccag cgacccgtat tttttgagca
    11581 attcgaccgc ggttttctcc cctaccttgg gaacgccggg aatgttatca gatgaatcgc
    11641 ccatcagacc gagtagatcg cgaatttgtt ccggggagac accccatctt gccacgacct
    11701 cctcgggccc gatccgctcc cattcgtttt tcgttttccc tggcgcaagt atcctgacac
    11761 ggtcgttcac aagctgcatc agatctttgt caccggtaac aatcgtcacg tccagcccgg
    11821 cgttttgccc ctgaactgca agcgttccga tgagatcgtc cgcttcgaat ccgtccagac
    11881 gtacaatcgg cactccgaac gcttcgacga tctggtcgat tctgggaagc tgggcgacaa
    11941 gttcttcggg catccgttcg cgagtcgctt tgtacgcggg gaaacgttca tgccggaacg
    12001 taggctgagc ggaatcgaaa actaccgcga gatagtcagg ggcctcctcg cggacgattt
    12061 tcagaagagc ggacacgaat ccgaacgggg cgctggtgtt ctcgcccttc gagttgatga
    12121 gtggattgcg gatgaacgca aaataggcac ggtatacgag tgccgttccg tcgaggagat
    12181 acaggcgctt cgcgtcactc accgcacacc ccttccgcca ggcgttcttc cgcacacgtt
    12241 gtcaaccgct ccattcgcag gatcatcgcg aactgaactg gcgagtcagt tcgccaaatc
    12301 gcctcacgcg agcgaccggg gtgtcgggag tcaccggact cccgagcgac atcacgaatc
    12361 tgccggtacg attgcctatt tccagcagcc gctcgatttc gcgtgcaagg agcgcgtccg
    12421 atccattctc caacacgtcg acagcgtcga gattgccaaa aagacataca gagtcgccga
    12481 caaccctgga aatcgtgtcc agatcgacgc agaacccctt cttgctctcc tcgaaggaaa
    12541 gacacattgg agagacggac acaaggtctt ccaaccggtc gctgacgtcg ccacatacgt
    12601 agaacacggt gatcatgccg ttctctctgg cgacgtccac caacggacgg ctgtaagggg
    12661 taacgaattc gcgaaaagcg gaaagagaga tagaatcgcg tccaacaagg cacaactcaa
    12721 cccatacgaa atctacaccg atcgcagcaa acgccttgat ttcctcgatg gcctttttac
    12781 acatccgctc ggagagagcg tgcaccaatg cagggttctc gaccatgttg gccatcatgt
    12841 tctcgaaccc gaagcaggta taggtccacg agggatggtt cactgcggcg tagactgcgt
    12901 gggaagcgcc aacggattcg accaccgcac gtgcgccatc gagaaaaccc gcgtcgagga
    12961 tttcgcgtgc ggatctcact tgcaccaccc gttcgatgtc ctccaggttg gtgactgatg
    13021 gacagttcac ggagtagtgg tcgccgcttc gaggccagtg ccgtaattcc gtttcttctc
    13081 ccgttaccgt gtttgtgagg tagggcacgc cgtcacgaac agatgctgca tgggtttccc
    13141 tgaactgccg tgaagctccg ataccaacac ctgtccaatc catacccgtc gattcgagga
    13201 agtcgcgttc cacctgaact gacgatgcga tattgccgtc gcttgtcttc caccagggtt
    13261 ctgtagttac ctcctcccag tgatcgcgca ggaagatggc atgatacggg atgactaccg
    13321 gtgcgcaatc cacagcagag cctgtacagg cagctcggag ccgttctctc ggtgtcacgt
    13381 tcgatacctc tcagcgttcg cagaactgcg gctgtccatc gcgctcccac tccaatttcc
    13441 agagtccgtt tgaatcgtgt tctgtgacgt agatggtccg gctacccctt ccaccgaagt
    13501 gcaggttcga aggtgccttg aacggaagca tgatgcgctc gatgagctca cccgcggggt
    13561 tgaacacgtc caaggaaccg ccgccccagt acgccgcgag cagattaccg gctgaatcga
    13621 aatccagacc atcgggacca agaccgcctt caacatcgac gagttgagcc cacttccgct
    13681 tgtttgcaac ctttccaggg gcaaggatat cgtacgccca cagccacctc gtaagagttt
    13741 cggccacgat caggatgcgg tcgtccgcgt tgacggcaat accgttacag aattgaaagc
    13801 cgccgtccag aagaacgagt tctccgttgt tcttcctgca aaagacctct ccgttcgggg
    13861 catctttgcc actccccgcc ggagccgtga catacaggtt gcccctggaa tcaaacgcgc
    13921 aatcgttgca gcctcgcatc ggtttgccgt cgaactccgg cgtttccaca cgtacgatac
    13981 cctcggggga tattgaaagg atgccgagtt tcatgtccgc aagccagagt ctgttgctcc
    14041 tgtcgcactg cagacctgcg ggaatgcccc ctgtctccgc aaatatcgaa gtctcgccag
    14101 attcattcac gcggaggacc tggccgaggt tgggcgcaac catgaagaac tcaccgcgca
    14161 tgttgacaca agggccctcg gcaccgggaa tgtctttggc tatcattttg caggggtacg
    14221 tgctggtttt gatcatcgcg cgctcctttt ggaatggcgt cagaggcggg tttcaaccta
    14281 cgaaggtacc ggcgggagac aacctgcgca accacgtgga agctcacaca tgatcctcca
    14341 aaatgacctt atccgggtgg aacttggcga cgaaaccggg ggattccggt ccctgaagga
    14401 cttgcgtctg gaccgggaat gggtgtgcgc ccctgatcgg agcctccttt tccggttgat
    14461 ggtgccggat ggtgaacgtg aatgcgaaca cgtagatagt gatgtgccgg cggttgtccc
    14521 cggagggtcg agctgcgttc tgacgttcgc ccggaacggg atcagcatgc aggcgtttct
    14581 ggaactggat ggagacgctc tcgtctgccg cctgcagatc gtcaacgatg gacccgcacc
    14641 ggttgaagag atcatgttcc cgtatgtgag aggtcttgcg cccgttgaaa aggcgtgcat
    14701 tacgtggcct catttccatt tccgcagaat tgacgacgta ttcggtagag ggctcggcgg
    14761 cgaccaccac tcgtggaacg agatggccca gaaatacgtg gggcggtatc cgcactggct
    14821 gaccacggca tggtgcgact atggcaacga cagctccgga atagccctgg aagggcggca
    14881 caaagatttc tcgataatgg atttctgcct gcacaaagtg gtcgagaagc atttcgatcc
    14941 cgttcggcgc tctctcgatc tgtacactgt gcacccgcac cgggtagcac ccggagagac
    15001 cttcacgacg cctcccgtac ggatccgcct gcatgaagga gactggcaca ccgtcgcgga
    15061 tgaacaccgg ggctggctgg acacatggat aggaaagccc gaaaggcccg cgcaatttgc
    15121 cgagtccatt ggatggcatt tctacttcat gaaacatcag gacggactcg aggtgaactc
    15181 ctacgctgac cttccgcgca tggcgaagtc cgctctggca gctggatgca ggtatctgat
    15241 ggtcttcggg tggcagacag gaggacacga taacaactac atgtaccgtt acgtcccaaa
    15301 cgactcctgg ggcggcgaag cggcattgaa agccgctctt gaggaatgcc ggcagcttgg
    15361 agtacaggtg attccgttct tcaacggcac actggcaaac acgcagatgc cggaacacag
    15421 ggaatacgga tatcgctggg aagccacaac acgcgctggt cacccgtatt acgccgggga
    15481 ctgggcacgc aacaattttg acgctccctc acgcaaccga tccatgttac accacgaggt
    15541 ctgcttctgc gaggaatatc gtccacattt tctcgacgcc gtcacgcgta tcgtcaagga
    15601 ctacgggttt ggcaacacgc aactggatca gattgcggag aagatgttcg tgtgctacaa
    15661 cccttcccac gggcatggag cacctgacag ggcatttgtg gacggtctcg ctgcgctcct
    15721 tccgggagtg cggaaaatcg tgcgagatgc gaatcccgat ggagtgattg tcggcgaggg
    15781 tttcaacgat tttgtgaacc aatggtgtga cagttcgtgg acgtggcgcg ggctgttcga
    15841 cttcccggaa gccgttctgt tctccctccc ctggttgttc gccagttgcg agatagatgc
    15901 ccttgaatac ggggatgcga acagggcttt cgcgtacaaa ctacaactcg acatgaagat
    15961 tgacgggggc gatggaaaca tcagcgacta tccggaattt tgctccttca tcaagagcca
    16021 tgccgaattg cgtagccgag tcgcgcccta ttacgtctac gcagatttcc gggacgaaga
    16081 gaatctggtt atacgcaaac cttccggcgt ctttgcaaaa acgtacctca accggaatga
    16141 agggaaagtc ggcgtcgtaa tagccgaaac ggccggcaag cctgccgacg ttgagtttgc
    16201 ccatacctgg ccaaccgacg aacgggctgc gcgagtgtgg tcgtcccagg gcgagatgtc
    16261 tcaaatcact gttgaaggcg tcgtcagaat ggagcttcgt ccattcgagg tacttgtcgt
    16321 gtgcctcgat ggcgtgcgta cgcgccatct ccccaacgtg aggaaggatc tatcctgaga
    16381 tggttgtagt ggcctttcag ggcgagctgg gcgcgttcag cgaggccgcc gcccgcgcgt
    16441 acttcggaaa cgggatccag ttgtctccct ccgcaacctt ccgggacgtc tttctcaaag
    16501 tcagcaccgg tgaggccgat agaggggtga ttcccataga gaactcgctt gcgggcagtg
    16561 ttcacgaaaa ctacgatctc cttctcgagc atagcctgca cattgtgggt gaggtcaagt
    16621 tgcggattgt gcaccatctt gtcgcaaaca aaggagttgc ccttccggat gtgcgacgtg
    16681 tgttctctca tccgcaggcg cttgctcagt gccaggaatt cctccgaggc ctgcccggag
    16741 ttacgccggt tcctgtgtac gacactgcgg gagcggcaaa ggagctgaag gcttctggta
    16801 tgcgtgatgc tgccgcgata tcaagcgcgc aggctgccat ggattacgaa ctggagattc
    16861 tcgcctcggg catcgaaagc aaccaccaga actttacccg gttcctgatc gtggctctcc
    16921 agccggatgc agacagggac aggcccggca acaagaaggt ctcggtcgca ttcgcactcc
    16981 atcatgcgcc ggggaccctg ttcaagagca ttggtgtgtt cgcattgcgg gatatcgacc
    17041 tcctgaaaat cgagtcccgg ccaatccccg gaaccccgtg gcagtatctt ttttatctcg
    17101 attttgcagg cgacgtagag gaaccccgcg tgggcaaggc tctagaccat ctgggcgagc
    17161 tcacttcgtt ccttcgcgtg cttggttgtt accctattgg ggacaccata gacagtgcaa
    17221 cgccgtttgc cagagaccga gctggacagg actcagaacg atgacatggc ttgagcgtgt
    17281 cggggctttc ctggcccacc ggcgcgtaca gagtatggcc gccgaacccg tggaaatccg
    17341 tgaagcactt cgcgcgggag gcaagactct cgttttcttg ccgcaggaac cggagcttct
    17401 ggactctggc gtttctatct gcgtcacgct cgccggctgg ttcggtccac tcgcgttgtg
    17461 ttgcgtttcc cgtgacgaca aactaccgga tctttcgaac tccggttatc cggcggttct
    17521 gttcccgtgg gcgatcagca ggatgggcct gcccacacgc aacgtacggg aaaaggtgaa
    17581 ggtgctcgcg cccaccatcg cggtcgacct tcatcctcag ttcaacctgc cgacagccta
    17641 cctcgcaacc gaaagcggag cttctttgag aatcggtttt ggcgacgctc cgtccggcta
    17701 tttcaacctc cgatatgcgt gtccctcatt cgacccggcc aattccgctc tgcattacgg
    17761 gcgattcctg cgcaccatcg aaaaccttcg tgctgacacc ttcgaagagc cccccagatg
    17821 agcattctgg agtcgttcgt cgcgctggat tttgagacga caggcatcga tcctttttcc
    17881 tccgaaatta tcgaagtagc cgcactgcgc gtacagagcg ggcagattgt agactcgttc
    17941 cagactctgg ttcagccgca caaaatgcca tctgtatcga tccaacgcct caccggaatc
    18001 accgcggatg agttgcaggg ccaaccgtca ctcgagggag tgatggccag gctgcggcgg
    18061 tttgtcggcg caacgcctct catctctcac aacgcggatt tcgaggttag tcgtctgcgg
    18121 atgagcatcc cggcggcgga attgccgacg gttttcgaca ctgtggagct ggcgagaatt
    18181 gctctgccgc gattgcccaa ccatcgtctc gagacgctcg ttgacgcatt ttccatcgag
    18241 aatccggaac aacaccgtgc gcttggtgac gcacagacac tcgtggaggt gttcagggcc
    18301 ctcgtgaaca ccatgaggtc cttttccccc gtgcgactcg acaccctggc aaaactggcc
    18361 cggagccttc ctcgcccgga acctccgttc ggagcgatcg cggatctgct tctggatata
    18421 caggccgcgg cggcgcgctc cgcattcgac caggtctccc cgccgccttt ccgggtcggg
    18481 gctttgacga gccttcccaa tacgttcggg gcgttcgatg acaacaggct ggaaccggct
    18541 accagccttg aagacattga cgcagaatca attgcggaga cactcggggc tcacggtgac
    18601 tttgcgcggg cgatgcccac gtatgaggtg agacggtcgc agatagacat ggcgcgagcc
    18661 gtcactgaga tactgaacga gggcggtacg cttctctgtg aggcaggtac cggtacgggg
    18721 aaatcactcg cgtacctcgt cccctctgta acgtgggccc tgaaaaacag tcaccgagtc
    18781 gtaatcagca cgcacacgaa gaatctgcaa gagcagttgt tctacaagga tttgccgctc
    18841 gttgcaggaa ccctcgacgt tcccgtgcgc tcagtggtgt tgaagggcaa gaacaattac
    18901 gtatgccttc atcagtggca tgaggaaatc ggcctgccgc ccgccgaggc ggtggatgcc
    18961 agccgggcgc tgctgcttcc gctggtttgc tggatagatg agacagagtc gggagattta
    19021 gaggaaaacg gcgcctttca gacactgctg aggtatgttc ccggcgcgta ctctcttgcc
    19081 gtccgccttt cgagcgaagg caaaccgtgt ggtcgaagaa cgtgcaggta ctatgaccgc
    19141 tgcttcgtga cacgtgtgcg ccgcgcagct cagcaggctc acatcatcgt ggtcaatcac
    19201 tcacttcttt tcagcgacct cgccgccgat catggggtta tcggcgagta tgagaacctt
    19261 gtcgtcgacg aagcgcacac gtttgaggag actgcaaccg actacctggg gatcgagttt
    19321 tctcttccgc ttgtgagatc cgttctcgat gaaataatca ctccgcatgc gcggagcctt
    19381 ctgacgcgga tacgaggcaa tcttcgcagg agcaagattg aagactcaac aaggaagtcg
    19441 ttcgaggttc agctcggggc gacgggccaa gccgcggaac ttgcaggtga aaaagcggag
    19501 gcgttctttc gtgcggcaaa gaatgagctc ggagcgcctc acccttcgtc tatttactcg
    19561 tccaaaatgc gatactcctc ggaggtgaac gtcttcaatg agatacggga gcatcgtgac
    19621 gatctttctt tcgcgttgaa aaccttgcgc gatgggctgc agaccctcgc cttgtggctg
    19681 agggacctgt cttcgggagg cattcccgaa caagatgagc tgctcgccga cacggaactg
    19741 cggcaagcag acgtcgctcg tctgacggcc gcgatggacc tgatcacccg tgccgaagac
    19801 gagggcatgg tgtactggtc cgagatgcca ccacacggcg acgaaaccag actcaagttc
    19861 atcgcagcac ctctggacgt tggtgcgacg ctgcggaggg atttctaccc gagtcttcgg
    19921 tcgctcgtga tgacctcggc aacactttcg gtcgcggaga agttcaacta cttccgacaa
    19981 aggctcggcc tggaagatga ttctgtgcga acgatggcgg tgaaatcacc gtttgatttc
    20041 gagaaccagg tacacatcgc ccttcccggg tggtttccag agccccggga ttctgctttc
    20101 gaaccggaag tcatttctct gctgcgagtc ttgattccca gaatccatcg tggcacgctg
    20161 gtgctgttca cttcccacgg gatgttggaa aacgcgtaca acgccctgaa acaaccgttt
    20221 gaggaacagg gcatcctgct tctcggccag cggttcgatg gttctcgatc cagcatcaca
    20281 aatcggttcc gccaggatcg gcaaagcgtt cttttcggca cagaaagctt ctggcagggt
    20341 atcgacgtac cgggagaagc actggagctc ttgatcattg tgaagctccc gttttcggtg
    20401 cctggcgagc ccttggaaac cgcgcagagt gaggctctgg ccgctgccgg caaaaacccg
    20461 tttcttcatc tggccgttcc gaaagctgcg atcagattcc ggcagggctt cggacgactc
    20521 ataaggcacc gcaacgacaa aggtgcagtg ctgattctcg acacgcggct ggtaagcaag
    20581 gactacggcc gcgcgtttca ccgctcgctg cccgcgtcct acaccgtgta ccgttctcag
    20641 gaggaactga tagcgggttt cgaagcgtgg tttgccgccg aaatcacaac caagctgtga
    20701 agaggccttt tcggtgatga ttgtctcgat cgcggacaat cagtacggtt ccgccgaact
    20761 caccgacgtg cgggccggta gaaatacgtt ttcaaatgcc ccaagttgat cgttgtgaag
    20821 gagtgtcaaa tggtacccgt cagagttgct gttaccggcg ccgccggtca gattggctac
    20881 agcctgcttt tccgcattgc ttcaggagag gttttcggga aagggctgcc tgtggtgctt
    20941 cagttgctgg agatccccca ggcaatgaag gcccttgaag gcgttgcaat ggaacttgag
    21001 gattgcgcgt tcgccagtct tgcgggagtc gagctctccg acaccccgga acaagcgttt
    21061 tccggcgcaa actgggcatt gctcgtcggc tccaagccga gagggccggg aatggaacgg
    21121 attgacctcc ttcgggacaa cggcggcata ttcgtcggac agggcaaggc gctactgaat
    21181 gctgcttcag atgttcgggt ggttgtggtg ggaaatccat gcaacacgaa ttgcctcatc
    21241 gcgatggcac acgccggaga catacctccc gaacggtttt ccgcgatgac acgccttgac
    21301 cagaatcgcg cgacggcgca acttgcgcgc cgtgccggag ttccggtggg tcaggtcacg
    21361 aacgtcacca tctggggcaa ccacagcgcc acgcaatacc cggatttcga gcacgcaagg
    21421 atcaacggcg atccggcaga gacagtcatt cgagatcgcc cgtggctgga aaacgatttc
    21481 accgagacgg tacagaagcg aggggcaagc gtgattgcgg ctcggggggc ttcgtctgcc
    21541 gcgtctgcag ccaatgccat catcgaccac gtgaagtcgt tcctcctccc gactccgggg
    21601 ggacattggt tcagtgctgc cgtcaagtcc gatggtagct acggcgtgcc tgcgggactc
    21661 gtttcgtcgt tcccgatcac aaaaaaagaa acggacaatt ggtgcattgt accggacctg
    21721 attctcagcg aatatggcaa gagaatgctc gcgaacacgg tccgcgaact ggaatcggaa
    21781 cgcgatatgg tgaaggacat gctgggttca tgaatgcccg gcggtgatgt tccacagtca
    21841 cgcaaagaaa gggaactacc gtggcgatac tgctgatgaa ggactgggtc gaagagcgtc
    21901 tcaaaactcc cccgcggaat tcctgcgcgg caacgacacc cgccgagttc ctcgagtggc
    21961 agagatcctt ccgacgttca atcaaacgtg cgttggggcg gttccccgaa agtgtaccat
    22021 tggctccaaa aacgctggag aagacggatt gcggcacgta cactcgcgag aaggtcgtgt
    22081 ttcacagcga gccggcaatg gcggtggtgg cgtgggtcct tacgccgaga cgcacaaaaa
    22141 gggggcaacg tgtgcccggc ctcgtatgcg cccacgggca cggacttggc aaaaacccac
    22201 tcgtaggcct cgataacaac gggcaggttc acgaggatta ccagcaccgg cacgccattc
    22261 agtttgcaga gcgcggctac gtaaccatcg cgcctgattg gcgtggattt ggcgagcgaa
    22321 gggacggacc cccgtgggac gataagggcc gtgacggctg caacctccgt cacctcgccg
    22381 ccggttattt cgggttttca cttcttgcgc tgcagatcca cgacgggatg aggtgtattg
    22441 attacctgca atcgcgaaaa gaggtggacc cgagccgaat cgggtgcgtc ggcgtatcat
    22501 tcggcgggac catgacgacg tatctggcgg ccatggaccg gagagtaaag gcggcagtca
    22561 tctcgtgcta cctgagtaca atcgtgaatg gcctcagacg cacgaatttc tgcggagcgc
    22621 agtacatgcc ggaattgcgc gcacacggcg acataccgga tgttgcgctg cttgcggccc
    22681 cacggccttt gctcgctgag gtaggtgaaa aagaccgctg ttttgaagtg gatgacgcgc
    22741 tccaggcatg tgaacgtgtc agggctggtt acgtcgccgc cggccacccc gatcgtttcg
    22801 acgtggacct gtttcctgga gagcacgagt tcagcgggcg caaagcgttc gactggtttg
    22861 atcgctggct gaagtgagat gagatcataa gcggtatctg gatccgcgcc agcgcgttct
    22921 ccctgatagc aggaccatga tggcaacgac aggctgatag atcacctgcc acagagtcag
    22981 aagtgggctc atcggcgccc atttccagtt cccgcgggca ccgatgaggg ctgcgtccgc
    23041 gaggaaactc gagcaccaca ccatagcagg gatgcgccag tcaagcactc cacaccatgc
    23101 catcgccgaa gtgagggcaa tccacgaccg gtgaacaaat gcaaacgcag gcaaaagcat
    23161 ttgcccgacc tgaaggtgac gtacgccggc cgcccagcga attctctgcc gcacgaaatc
    23221 tccgattgac gagcacgggc gagtaagcac ggcggccgta tcccgggcgg tagttatgct
    23281 ccacggtgtc gcagcgacgc gttgaacgag cgcacagtcc tccgtgatcg tgtagccgag
    23341 attccggtat ccccctacgt cctcgtacgt cttgcgcctt atccccagat tgttgcctat
    23401 cgccgatacg ggacgcccga gcgcgatcat tccgaatgca acgctgagga gatacgcaag
    23461 ttcgcacttt tccagcaagg acgagatcga ccgggtcgtg ggaatcgtcg tccaacctgc
    23521 caccataccc acgcgggggc tggaaaaggc tgccaccaag tcggcgatcc acgaagaagg
    23581 cactacacaa tccgcatccg tgaacaggat gatgtccccg agtatgccca ttcccgccat
    23641 gtgctcgatt ccagccgcaa gtgcgttctg tttccctccg taagcaggat cgcactcatc
    23701 cacgcgaagc gcatggatcc tgtcgttttc atctcgagcc cgacgaacca cctggccagt
    23761 gccgtcctgc gaccgatcat caacgacgac cagattccac gcgtccggcg ggtaatcctg
    23821 cctgctgaga ctttccaggc atacgctcag cgcctcctct tcgtcacgtg cagcaatcac
    23881 tacggtcacc gacgtattcg ccgcggaagc gggcaccctc acccgtcggt tcccgcgcag
    23941 ggccccggcg agaatccctg cgcttaccac gtagcccaga gcgagaacga aggcgatgat
    24001 actgattgcc agcacgtgac ctcaaatcca gggagagaga cggagcccgg gctgacccct
    24061 ttgacccgac acttcacttt ctcccattgt gattctgttt cttgcgcggc gccagtacgt
    24121 tccagacata cggggaaatg gacgtccaca cgtggacggg cgaagtgaac gcgctcaccg
    24181 cgtagtactg gtaccagccg caccgccggt cgcacgtggc catttgccgg cgaaacgcct
    24241 tcgcctcttc gccgtgcagc cagtcccgaa gttcaacatc gaggaggttg accggcttcg
    24301 attccaacgt tctgcacgga tagaacagcc ctccgtcgca gtcgacgatt atcgatccgg
    24361 ttgaacaccc gtgtggctca cgaagggcgg cgaaatacgg gtccggcgat attatcgtcc
    24421 gggggtactt tcgtttgagc ctgagacact cgcgctgaaa cgcatccagg tcagggaagt
    24481 ggtttcgggt tcggttcaac tccacggccg gcataatgac acatttggct ccggcgtctc
    24541 tgcatctcgc aactttgtcc tccaatgccg gcaggttctc tttcatcaca accacctgca
    24601 cgcacacaat gctgccccaa cggaccgctt ccggcagcag tgagaacatg tccagattat
    24661 catgaccttc atcgatgctg atgtggagga aatcaatgtt ccggcagtat tcgaccatcg
    24721 gctgttcaat gagggaacgg tcgctggtgg tgaagagaat gtagtaatcc tttgctcccg
    24781 cgtacttgag gatatcgccg atgtcgtttc tcagaagggg ttcaccaccc tcgagtgaaa
    24841 gcagaaatac tccggcacgt tccaggttat caagtactcg gtacaccagt tccgtctcga
    24901 gcaccggcgt cttctcaaac cacacattgc agaacttgca ccggaaattg cagcgattcg
    24961 tcaccttgta tgaagcgtac agagggttga ttgcttcctt tttccgaatg ttttcgccaa
    25021 ttgccgaggc agcgcggagg tacgtcctca acggcaacga tttcatggaa gaaactggca
    25081 tggcagacca cctcaaaaca gaaccagcga ccgtatgatc gcgcgtctaa caacgtactc
    25141 gccgcggttt ccccgtgcaa gcttagcctg ctccccgatt tcccaccagt gacaaaacga
    25201 tctgccgatc aggcccggaa aacgacacgc tggccgattt cgcggcatgg ctccagagag
    25261 cttcttgatt atctctaaca aacacattac tttcagttta gcccacggca tcgcatagta
    25321 ctctctgctg tccgccagat gacgaagcca tggggtgccc ccgcatcagg gatacgccgt
    25381 ccacagagca aaccgtgagg ctgtcggccg caccgcgcac cggcagacgg gattgaatca
    25441 gaattgcccg aagggcgttt atggagccgt ccatgtcaga gcgaggcgcc gtcctgattg
    25501 tgggagacga ccaggcgaca tgcgagaccc tctctgattt cctcacatcg gcgcaccaca
    25561 ccgttcacgt cgttacttcg gctcctgaag caattaccga actccagaac cggccctatg
    25621 acatcgtggt gctggatgct ctcatggcag gcgaaacggg tctggacctc gtcgccagga
    25681 tcaaagcagt tgacttccga cttcaggtca ttgccctgat ggatatcggc gcgggtgaag
    25741 ctgcggtacg agccatttcg cagggcgcaa gttcgtatct ccttcgaccg gtcagactgg
    25801 ctgcgttgct caatcgcgtt gcaacggcgc ttcatacacg gtcttttttt cggagccttc
    25861 aggaacccgt ttccgatccg gagcgacggc cagatcttgt gcgcggactt gagattgcgg
    25921 aaaaactgtt tcaattcgat cgggaacttg tctcaatcct ggattaccat ctcgtcgtcg
    25981 agtccgtcct gagaagcgcg ctctccatcg ccgaagccga catgacagcg ttcctcatga
    26041 tacgtgacag agtggtttcc atcgtggtga acgtcaagga ggactctccg ctcgcaagtc
    26101 gacaggcggt gttcgaacgc atgacctcga aatgggactc atggggcgga aattcgctgc
    26161 acttggacga tgttctctgg tccggactcg aggaagacgc cctcggctcg acacgggaag
    26221 cgagcgtgta cccgatcgtg gtccaggacg tactggtcgg ggcaatcggt gcgttcgcaa
    26281 cagggcgccg atctttggat ccgcgtgccg atgttctggt tcccattatc gcgagccgcg
    26341 cagaggtagt gatcgagaac gcatttctgc acgaacacac gaagatcctt gcaacaacgg
    26401 atcctctgac gggcctgctg aaccgacgcg tgttccgcga aggaatcgtt cgcgagttcg
    26461 agcggagccg acgcctgttc ctgaacaaga gacccggtgg cgatttgtcg gttatcatgc
    26521 tcgatgtgga tcacttcaag aaattcaacg acacgtacgg gcatcagctg ggcgacaaag
    26581 tcctcaaaat ggtcgcaacg gtcctgttga atgttgctcg ccgcgcaacg gatatggtgg
    26641 cacggtacgg gggagaagag ttcgtcgtca ttgcacctga cacgaccatg gaaaatgccc
    26701 gcatcgttgc ggaacgtatc cgggagactc tgcaggaatc cgtcatcgag tcggaagctg
    26761 gaccccttcg agtcacggcg agttttggag tcgcttcgta tccaaggtgt ggagcggaaa
    26821 cagtcgagga attgatcgag caggccgacg aggccctcta tgcggccaag cggaaagggc
    26881 gcaacagggt cgaggaagca gcctccagaa ccgagtcatc cacccctccg acacaacccc
    26941 agtgacggta tctgcatgat ttcctccatc tggtctcgcc tgagacatgg gctttcccgt
    27001 accagaagca ctctcacgct gcgcctccgc tccatccttg gtggagcact cactgccgaa
    27061 actctggaaa ccctcgaaga ggtccttatc acggcagatg tcggcgtgat gactgccgca
    27121 aaaatcctga agcggctgga gacggatgcc gcgagcagtg ggggcgaccc cgcgtgggca
    27181 atgagatccg tccgcaccac ggtagaggag atactgcgcg ctgcacacag gccattcgca
    27241 ctgcctgagg aaaaacccgc ggtcgtgctc gtggtgggcg tgaatggagt ggggaaaacc
    27301 acgacgatcg ggaaacttgc cgcccgttac gcagagcaga aaaggcgcgt actcatcgga
    27361 gcggcagaca cgttccgggc ggcggctgat gagcaactgc tggtctgggc tcaacgcgcc
    27421 agggcggaga tcgtacgaca gcagcacggg gccgacgccg ccagtgtggc gtatgacgca
    27481 gttgcggccg cgcaacaccg ctcagcggac ctggtgctga ttgacactgc cggaaggctc
    27541 cacacgaaat ccaacctgat ggaggagctg cgaaaaatca aaagagtgct cggcaagcta
    27601 tctcccacgt ttccgcatca cgtgcttctt gtgctggacg ccacgacggg tcagaacgca
    27661 cttgttcagg cccggcaatt ccacgaagcc atcggagtaa ccggaattgc gttgaccaag
    27721 ctcgacagca gcgcccatgg ggggatagtg atcgcgattt ccgacgagtt gcgggtacct
    27781 attcagtttg tcggggtggg ggaaaccctt gacgatctcc aggatttcga cccgcacgcg
    27841 ttcaccgagg ctttgttcga catcgggcag gattgaggtt ccacattccg gccccaaatt
    27901 aaacggggga ggcatttccc gcctcccccg ttttccggaa acaagccctt ccggagacct
    27961 aaaggtcgct gcctttgcgc tccttttcgc gcagtttcgc gatcaacccg ggaatgcctt
    28021 catcccggat gatcctgttg aaggcagaac gatagttctc cgcagtgctg acatcatagt
    28081 ccgagatttc tgtatccaat ccgatagtcg tatcccacac ccaccaccgg ccattgacct
    28141 tgtgcatctt gtactgaatc gccactttct cgcccgaagt ggaggtgact atcgtcgtaa
    28201 ttacggtatt ctgcgcattc cccgtcggtg ctgaatacgt cgtaccggcg gccctgtacc
    28261 gctgcaactt gcgaacggtg gagcgctgca cgagtgaccg gaacacagtc aggtaatctc
    28321 tctgttgctc tggggtcatc gcattccagt gccggccaac agattcgcgt gccagcctgt
    28381 caaaatcgaa cgcttctgtg attgcgtcgc gaagtgctgc ctgccgggcc gtatctcgtg
    28441 gagccctcgc aagcgccgcc tgggcctgct caatagcacg ctgtcgttgc ttcaaagcct
    28501 gcaaggcggt ttgttcggcc gcctgagtgg tgtacgccat tccgagtatt actgcggcca
    28561 acagggccat cgttttccac atgatacacc tcatatcgtt tgtggcatca ttcttctggt
    28621 tgtggttcgt aaggttcacc gttctccccg gaattttcgg gttcgacggg ctcctcaaac
    28681 tgagcatcgt ctccgtctcc ctcgccctcc agttccgaat ggccaacccc attcgttccg
    28741 ttggtaatct ccgagagagg ttttccaatc gttcgataca cttcaccaag agcgaggtaa
    28801 tgactgtaaa gagtctggta gtaatcgccc tgagacttcg cgtacgcccc gtaggcttcg
    28861 agcagatccc gcgttgaagc gacgcccatg tcataccgct ccagcgtctg tatcatgagc
    28921 gagcttccgg ctctgagcga tctgcggccc aattcactgt tcctgcgggc ttcatcagct
    28981 tcgaggtagg ccttctgaac ttcgagggct atccctgcct ccgcccattc tcgtttgcgc
    29041 agcatttcgc ggtactcgac ctcggcacgc tgaattcccg cctctctact gcggttgtcc
    29101 aacgtatact gcaaaccgag tgccgctttg acgctccggc cgttgtacgc atcgccgaca
    29161 aacgggttct cggtcaggag ctcgcgccca ggggcagcgc gcagaccaac ctcaacgccg
    29221 acgaacggtg tcgggtaacg ttcggcgcgc gccgcgatca attgttgccg cctggcttcc
    29281 acagccgcgt tgacctgagc gagttcaggc cggttttgga aggcatatgc gcgtagcgag
    29341 tccagcgaag gaagtgtttc cggaatcggt cgcaagcgat cggtaacaac atcaaaatgg
    29401 gcatcataag ggatgccgag cgcgcggcgc agtgccccga gcgccagttc cacgcttttg
    29461 ttcgcttcaa tgtgtttgcg ctccagttca tacgtgtaca catcgatccg catgagatcg
    29521 gttgtcgtaa cccggtcgga atcctcctcc agcatttccc tgactctggt cctggcctgc
    29581 gcgattttct cgctcacatc gtcgaggatt tcaagcagct cgcgagcaac gagaacaccg
    29641 taatagaggc ggtacacctc cgcggccacc gatgcgcggc gcacttctac accggcctcg
    29701 cgtgccgcga tcccgttccg ggctgctcgt ttcaaggcat tgacacgacc cagagtcgaa
    29761 attggctgag ccgcctgcag gtggaactcg aagcaaggtc cgaagttgtc ccacgacgtt
    29821 gtgagcggat gatctgtcac gtatcccctc gcgtccttca cgacgctcag gtaacttctt
    29881 gcttcgaata cgggtaacca gaaggcgcgg ttggcttcca gttggtcgat cagagcgcgg
    29941 tcccgttcca actgggcctc ccggatatcc aggttcccct gcattgcgcg gtcgagacac
    30001 tcccgcaagg tcaacctgag agtatccgtt ggcggtgcct ccgctgaggc aatctggaaa
    30061 ccacagaaaa tgaggacaaa ggcgagaagg tatttcacaa gagcgttcat cagttttcgc
    30121 accccgatag aaggatgcgt tgccttacat ttgccagttc gcacgcgtgc gaaccagggc
    30181 aaatcggaac ggacagaacg aatggatcag agcccacacg aaaagcaaaa cctgtgcaca
    30241 ctctaccatg cttgcagtgt gggggagcgg ctcgtcaact gcgggctatc gaaagcaatc
    30301 ccgcgcaaat taggaacaca cccaccacct tccagagtgt gatcggctcg ttgaacacca
    30361 gagccgacac gaatgttatg atgacaatca gtaacgccgt gaaggggtac atgtaactca
    30421 ggtcgagccg cgagagcgat atcagccaga ggaatgaaga tcccgcatag aaaacgaggc
    30481 ccacgtaaac catcgggcga ctgaggatgt tcctcgcggc ctccaccggt gatgttacgg
    30541 gaagcggcgc accgaacatc gcgtacttca ttgtgacctg gccgaaagcc gccagaatga
    30601 tggcgataat caccggcgcg agactgattg cttttgccat gacgcaccct tcatggggtc
    30661 acgcgggaac caaaacagcg cgggctcccc gtgcgagaag cccgcgtttt tgtcatcgat
    30721 ttgccccgga atctactgcg ttgtgcttcg tgtctctaag cttcgttatt tcttgaatat
    30781 atcacatcga gcgcggtgtc ggcaatgtgc ggactccgcc ggcgccgatt tggttctcac
    30841 gcgggccatc gttccaggca cgaagtcgaa aaacgtgcga gaaacaaaag gggctcccac
    30901 cagatcgcgg gagccccttc gtaatccgtt cttgggacag gcgtcaggct tcgaactcga
    30961 tagtcatgat cttctttttc ggaacatcga gggtcagaac gttccctttc ggctccagat
    31021 cctgacgccg ttcctcgttc atattgcaca accatgcctt ctttatcggc ctgtagaacg
    31081 tgattgttcc attcatcgcg tcgccagtgg ggttgaacac gcgcaaaatg aacgtgtccc
    31141 tttcctcagc ctttttgaag gccgacaaca cgaggttggc cggggaaatc gaaaggaagc
    31201 tcatgctctt cggaaggtcg cccttgtgcg gcccaacttc cgccacctca agcggcaggt
    31261 tgatgtgatc tgcctctttg tacacctctg cagccgccca gtctcccttg tgcggataga
    31321 tgttgtacct gaactccatc tcaccgggac actgggaaag cttcatctcc ggatatacgt
    31381 catacgtgtc gaggatgggt ggatttcgga atgtgaaagc ccggaaaagg gtaagcgcca
    31441 ctgtccggtc ggcatcttcc attgcttcgt attcgcgaat accgttattc aggaacgtga
    31501 tgccgctttt gccgtttgag aagctcacaa aacggtgcat cggcaacacg ggattcgggc
    31561 ggccccagta ccaattgtcc ggtgtgcgga tcacctggcg ctgaatgacg tcgaacgagg
    31621 cttctgagtc cgacgtcgct gcctggatac cggtcgggaa gcatacacgc aggcggtggt
    31681 tttcgcactc gttgttgacc ttcgtgtata cttccagcac cttcgagccc ttgcgcaggg
    31741 tgaactccga acgaatcacc atctctttcc gccgttcgct tcgcttgctt atgcgcgggt
    31801 ggcggatctg tgcacggggc gtctggccgg tgtacgcctc catggtttcg aaatcgagcg
    31861 cctctggaat catcatgtgg tattctacgc ggaaacgcgc caaggccggg ccgctttcca
    31921 tctgcgcaat gtatacgttc gcgccgtggc tcgtgatgat ctcgtcatga tccgggggga
    31981 tggtcgtcca ggaatgacca ttctccccac cgtcctccag gtaatgcatg ttgtcaaata
    32041 catcaccggt ttctttgttg gttatctgaa gagtgccatt ggcgttgatt ttcactttga
    32101 gatgctcgtt ttccatcgtg ttgacggccg taacgagcgt cttcatcggt ttcggcacga
    32161 cgtcttcttc gtgtttgacg gtgtaggttc gatatccgaa cggaggaaca tccaaggctt
    32221 cgaagtgcac ccgcacacgt tcggcatgaa gctgcaggga gacgtcgcgc aggttgcgca
    32281 ccaacgttcc ccatggatag aactccgcgt actgcgcttc aaccttgttc cctttttcgt
    32341 cacgtatggt gaaatcctca tatttgcaca catctggcag atccaggagc acgctcacca
    32401 cttcgcttcg cgggaatgga gacgggttga acacggtcag ggcaatctcg ttggcggcaa
    32461 cgtcgctgtt gtcaatccgc gtctgaatct tctgcaatgc ccggcgatac aagccctcgc
    32521 tgaagatgtt gacctgttcc ccgcgggcga tcatatcctt ttccatctga tccacgccgg
    32581 caccgcagat ggtgtcgtgt gggtgatttt tcatgatgtt gtcccagcac tcgtcgagga
    32641 cggttgtggg gtatttggca ccaagtgtca tcgcaagggc acaaaacggc tcggccttgc
    32701 gctggacgtt ttgctcgttc gcgttgttca tgagtttgat gcgcacacgg ctggaaatga
    32761 catcaccgaa aaggtgcgtc cacttcccca cagagttcgg gttccggctc tcgccttcaa
    32821 ttacctccgg cttcaaccga cccgcagcaa cctcttcctt taggcgatcc atgaactccg
    32881 cgaggtcgga ctgcttaatt acgtggttcg ggaggtgttt ctgtgtctct tttataaggt
    32941 cgagctcgcg gggatcgggt tcggaactat caaaaccctg catggacgcg atataccgcg
    33001 tgctgaaatg ctgggattcg tcgcgcagga gtttccttgc gccagtatcg atgtgctccg
    33061 tatgccagcc cgtcgcatcc ggatcgcaaa tgtagtaatg cgcgttcaca tggtggtcac
    33121 cggcaagttt gaacgggaga ctgccgcgga accagtcgta tttcgcttcg tcccgagaca
    33181 tgttgtaggc gaccattcgg aagagataga agaaatagct gaatctggag agcgcaccga
    33241 accgcatacc aaggagttcg ctgccatccg ggccacgcat gatgaattcg ctcttctccg
    33301 tcttgatgcc gcggtaaaag atgatcgtgt cgatgtcgaa gcctctatag atctggggca
    33361 tctggcttgt ctggccataa ctgaacggcg tgtaaccgac tttcgatact tttccaaggc
    33421 tatttgccac cttgtgaccg atcaccagat tgcgcacgag tgattcgccg ttgacaatgt
    33481 attcctccgg caggctgcac catggtccga tgatcagccg cccgcttttc acatggcgtt
    33541 caatctgctc gcgtcgttcg gggcggaagt caaggtagtc ctgaatgcag agtgtctggc
    33601 tgtccatggt gaacgaatga tagtcgggct gcttgtcgag gagatcgagc atgttgtcca
    33661 tgaactcgag catcagcagt ctggtttccg ctgagggata aacccactcc cggtcccagt
    33721 gcgtgttgga gatgatgtaa atctccttgt ggtcgtattc actctgatcc ttcagagtcg
    33781 ggtcgtacat gtcggtctgc tttgccatca attgctcctt tcggaacggg caaccgtcgc
    33841 tggatcgccg ggggaattga actacatcgg gatacccggg caatgccggg atcagcagga
    33901 ataatatagc gattcgggct cccgggacga accgggaacc cgaatcgttc aggttttagg
    33961 gagtcggcag agccgtgccg ggagtgtgca ccggccgttc cgcgagggaa ccgtccgcaa
    34021 gcctgatgtg ggaggtgaat ccccgtactc cgtctttgat ctttatcacg cgggcgccac
    34081 gcaggaaccc ttctttgccg taaccgcggt acccagaggc gcgcccgtaa cacagagtaa
    34141 catcttccag agtaccctcg tagtcgttcg tgtggtcgtg gcctacgaaa cacccgttca
    34201 cacgatactc aacaaacgcg gcgaacgcgc cttcgtcttt accctgcatg cagacctttt
    34261 cgttcttgta accggtgcat gtgcgtgcat cccaaaccgt ctgatattgt gcaaacggta
    34321 tgtgaaaaaa cgccagttgg gtgaggttcg gttgggtctg ggtcttcagg cgttcgctcg
    34381 tggcgcggaa ccatttgacc tggttggcac gaatccaacc gtacccggac ggctccgagc
    34441 ttcccgcgcc agagttcatg caccagagga gggcactaac cccacggcct tttcgtgaaa
    34501 gaaccggcag gacatagtta cctggttgat tgaggctgtc gggtcccgac gtatacaggc
    34561 agttccgcgc agtggcaatc agactatcca ccgcggcgta cccccgcacc tcggcgtcgt
    34621 ggttgccgtg cacaaacgcc cagggcacgc cagtgctgtc aaagaaggca atcaggcggc
    34681 gccaggaggg tacagcctgc ggttcccacg cgaatcggac gatgtctccc gtcagcacga
    34741 taaggtccgg gttttcccac cggattatgg agtccagcag cgcgtacgtg gagtcatcgc
    34801 gcaagccggc atcacgggaa ccgagatgag tatccgtgaa ctggacgatg gtaaagacgc
    34861 cattgtcgtc aaatcgaaga acgtgagccg cccgcccccg ttcgccgagc gggatcgtac
    34921 ccaaacaacc agagacaacg aggagcaaag cagtacaaaa aaggacaaat gggcgtttca
    34981 ttccagacat ctctttcttt ctggccctgt ggaaaaccag ctctgggcgc caacttcttc
    35041 ttcaagcgcc ctgcgctcaa ctatgcgtct tccatgcatc ccctgaggta ggctgcaaac
    35101 ctgtcaactc ccacaatcgg atcctcgggt tcctcgaatt cgatgttgat gtacccgcgg
    35161 taccccgcat tccgcatgat ccgcaccact ttgcggtagt ccaccttctc gcgcgcctca
    35221 ccctgacgaa cggttacctt ggtatgcgtt gttacggcat acggagcgca ggcttcatac
    35281 tgcgcgtaaa tatcaccggt aaaattgcca aaatccagat tgagcttcac ccaagggttg
    35341 ttcagcggct tcacgaggcg cagcgtgccc tcggcatctg cagtgactcc gccgtggttc
    35401 tcgagcgcca ggattacgcc ttctttctcc gcaagtctgg cgcactcggc aagtccatcc
    35461 cttaccatct gatcggcatc ctcccgcgcc atcccttcgg tgacgtatcc tgcgaacacc
    35521 cgcagcgcgg ttgatcccat ccacgccgat ttttcgatcc aggatttgac attggcgatc
    35581 tgttttttgc gctcttctct cgtcggttgg atgaaattgc cgccgacggc cgtggcggaa
    35641 atgtccaacc cgcgcacgaa tgtctcccgt ttgatgtgga ggagatattc tttcgtcgtc
    35701 tcggggaaat agtacgcggt ccattcaatg ccatcgaatc cgagatcgcg aacggtg
//
LOCUS       DAOTNG010000003        25857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_1982_length_25857_cov_5.514573, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000003 DAOTNG010000000
VERSION     DAOTNG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 25857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25857
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_25857_cov_5.514573"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..583)
                     /locus_tag="P5179_00290"
     CDS             complement(56..583)
                     /locus_tag="P5179_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRS93690.1"
                     /translation="MAMVQVVVHGLGLDVNHGPVVLLKEAEGERVLPIWIGHPEAHSI
                     QMKLENQDCGRPLTHDLMKSVMDLLEARLLRVEITKLEDSTYYAELVVRTDAGEARID
                     ARPSDSIALALRFDAPIWAEDGLFRSPETEQSDTEAGQPEETDEQRAERQKTELQRRL
                     RHIDPSSFGYYRLGD"
     gene            complement(636..770)
                     /locus_tag="P5179_00295"
     CDS             complement(636..770)
                     /locus_tag="P5179_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93691.1"
                     /translation="MTRGLIHGFSLLGRDSVEKRELRVEMVAAQRDVIDRFRRPAYID
                     "
     gene            792..1616
                     /locus_tag="P5179_00300"
     CDS             792..1616
                     /locus_tag="P5179_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS93692.1"
                     /translation="MQFRSSGHVEQNVARIVDHLRDLSRQGVCAVAFPECAVTSYDEK
                     AIRATAPERLSQAEKEIAVACRENGIGVVVGIPYWEQGTLYNGALAFDSGGKCVARYA
                     KIQLAGEKWCAAGARFAMFSFDSVPCSVIICHDERYPELVRLPVLAGAQIIFYISCES
                     DITAETKIAPYRAQIQARAMENSVFVVHANAPMGNVRMRNGKPRGEAAASNGHSRIIK
                     PDGNIVAEASIWGEDVLVADLDMSTATRHLALRSLESPLLHSWWEAGLRLVPPPTR"
     gene            1756..2268
                     /locus_tag="P5179_00305"
     CDS             1756..2268
                     /locus_tag="P5179_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93693.1"
                     /translation="MRVSPQSILLAAVFLFASSGSHAEVQSPNEARASIPADCEAGRV
                     CAMNDGVLDPVLASLLSDMGGLPPRARIRVFTPADIIAATNFDPLTISTAAVTWIVIA
                     QTGERGPVHWARASITPQGIMVKRGVFFPSRKAYAYSLARSVAVRFGICRGEDQSTGA
                     SFPVFMGNLP"
     gene            2342..3274
                     /locus_tag="P5179_00310"
     CDS             2342..3274
                     /locus_tag="P5179_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRS93694.1"
                     /translation="MTNNEVVELLAHDMSMEHQAILQYLFAAWVIGGEVGPSIESVAR
                     DEMRHFKYFGLAIAELGGKPNMEGPAFQRLTDPTAIVLANIASEDEAVRVYTQHRDLI
                     PDPRIKKLYDRIIEEEMYHGQKFQRMVPKVEGMPPVEFHEPQSPQQETLFRFLEEDIA
                     GEYAAILRYLHQSFVVADGKLGNAIEDRAIDEMKHMGWMAEHKIDSGGEPVLQPAPVQ
                     YSTELSEIFQYNIQLEKGAEERYQRHIDGYEDPDLQRVWAHIKFQEEHHTDDFENRLA
                     DLNRAPEAQAATPPDASNVKPKTSVGSLFGKPQS"
     gene            3332..4132
                     /locus_tag="P5179_00315"
     CDS             3332..4132
                     /locus_tag="P5179_00315"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HRS93695.1"
                     /translation="MADYLLRENAPLTEQEWAKVDELVTSVASRLLVGRRVLPLFGPL
                     GAGVQTVPVDTFDLASEEGIRRSGRVFVVPRMIAREFILGWQDIETGRSGVLPLELSP
                     AAAAASACALAEDTLIFRGDAESDGILNAEGCLGTKMSDWATEGAFNDILAAVQKLQE
                     NGITEKPALVVSPAGYALMQKPFGNTGALELAFVRELMPAGVFQTPVLKPSEAVVIAT
                     GPQNADLAVGVDLSVGYVDQVDMDHRFRVLETLALRVKRPQAICTITR"
     gene            4153..4488
                     /gene="rnpA"
                     /locus_tag="P5179_00320"
     CDS             4153..4488
                     /gene="rnpA"
                     /locus_tag="P5179_00320"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRS93696.1"
                     /translation="MRRETETTRWIRTVREEIPARRGSFLVAWVDKSSQRESPVVIVG
                     RTVGKAHERNRVRRRIREALRACVANPAGIIVVARTRARAADYWNLLNELEGLLAESD
                     ARMGGDRSD"
     gene            4457..4669
                     /gene="yidD"
                     /locus_tag="P5179_00325"
     CDS             4457..4669
                     /gene="yidD"
                     /locus_tag="P5179_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRS93697.1"
                     /translation="MLVWAAIGVIRIYQSLFSPLLGASCRFFPTCSSYGIEALSRHGF
                     FRGGWLTLKRILRCHPYHPGGYDPVP"
     gene            4685..6412
                     /gene="yidC"
                     /locus_tag="P5179_00330"
     CDS             4685..6412
                     /gene="yidC"
                     /locus_tag="P5179_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRS93698.1"
                     /translation="MERNTVIALVLIILIWILWPKWIKWTSPETQPVVPAALPADSSR
                     GPSSIPDSIVRSAPRPVVAASDTTRVRAIAHADSVPVRTCTIITDKYEAVLSNEGAVL
                     RKWSLKDYHTPYGAPVQLVAPGGHGISLSVGSEGKEIDLREKVFVPDTVFQMAHLTGK
                     DSLAVGFTCTLPNGRRVFKRYVFYGDRYRVAFAAGSADSLTAERMVLSWEGAMPSTEG
                     DFRREAGSMFVNAYVGGSLEKLHTLDKTETSSFTGQTDWVGVRNKYFLAAFAPTITDR
                     WDTRLFGSGVKDTSLRYSWRIAPFDLTKSTVTGFLYLGPLDVDILTAEGHNLERAADL
                     GWSVLRPISKLILWFFLSAYRFIPNYGWVIVVFSVLIKILLHPLTKKSYESTSKMQKL
                     KPLMDEIREKYKNDQQRLNQEMIKLYKEHGFNPLGGCLPMLLQMPIIFAIYAVIGNNI
                     EFRQAPFIWWVTDLSIPDSVYTLPFHIPLYGSHVTILPILMAISMFFQQKLTVTDPKQ
                     QALVYMMPVIMLFVFNNLASGLVLYWFLFNIFSSAHQYWLMKKQNGGEITPKTAPVPV
                     KNVVRPRKH"
     gene            6477..7853
                     /gene="mnmE"
                     /locus_tag="P5179_00335"
     CDS             6477..7853
                     /gene="mnmE"
                     /locus_tag="P5179_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRS93699.1"
                     /translation="MTDTIVALATPSGEGGIAIVRLSGTRAISIADSLFRAPRRLADA
                     PTHTLLYGRLALNDSAASPGSGVFDEVLVSVMRAPRSYTREDVVEINCHGGIRLADEV
                     IRACVSLGARRAERGEFTERAFLNGRLDLAQAEAVLDLVKARTKDALSAAYYELNGGL
                     SARINALRDRLTASLARIETGLEFDEEDLGVGDPRAESAALREILSEISALVGTYARG
                     RLIAEGASVAIIGPPNVGKSSLLNAILGEDRAIVSHIPGTTRDLVEGEADLDSVRVRF
                     VDTAGLRETGDAVEREGTKRAGRAASAADVVLLVCDGSGEIPWDLTPVSGRNAIIVIN
                     KMDKARNEVLSVLSASAGSTPVFRVSALSGEGLDTLCRGIRDALCADAPEPKTGGVII
                     RARHADALSAAGSHLERALDEATGQARSELVAADIRLALDALGSITGETTPEDIIRRI
                     FSEFCVGK"
     gene            complement(7888..9027)
                     /locus_tag="P5179_00340"
     CDS             complement(7888..9027)
                     /locus_tag="P5179_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93700.1"
                     /translation="MEPHERVLVVLLKSTTDWDIARDRNWYRIPAAHAPKDPGAEWIA
                     FYQPAGFGRERWGVYSYARVRGTHTVRRIELFPSETDHPRAQEPYLVYSFDPPARLAH
                     PLQCARPRRFVWTMTTWWRFSNARTFDDLLRRDPLLPPEKDTVLVSVVPRVSDFEIVT
                     KKHWYRIPCSVVSQWATPAYLAFYHGQGFGVEAGQVRYFAPIRYVDIVKRVDLFPDEP
                     KHPRARADYYRVHIDDLQQRPSPIRSKRKRQVVLIPTTFERFMLAGDINELAVGDEAT
                     DSFYGRMTEEGLRPERAYHVRGSNAYYFTDFAILGRKGSVHVDVARGENQNGFLPKRA
                     GRHAETPDGWTALRLSRFDITKRREDAIERIRNAVAAGAGSVMVR"
     gene            complement(9053..10201)
                     /locus_tag="P5179_00345"
     CDS             complement(9053..10201)
                     /locus_tag="P5179_00345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS93701.1"
                     /translation="MEGPRACRPEELDGLRTLENTVFRSNGESCMFREFPTLFHESNC
                     ERLRVFVDKGQPVSAIAYVSRTAVIQGNTLRIGSLGAVATFEEYRGHGLATALLEDTF
                     AQMKRENVDVVLISGQRGLYQRAGCAIAGHEMRFSLDGSALAGAPERGLVVEEAADAD
                     ISDLIALHQNEPSRFVRSPWEWQQFLSVYRLTPKGAEPPFGVRRLWVVRQQGTPVAYF
                     VLSVGRDNTNPTAYVQEFAGNRAAVLAGLALLKERFGLRTVSGSCLPSDREMSGLLTG
                     LGGSVQASSIGGHRMSFLNPEVLRRYDLWVTERLGTEFVGELNLVQSDDVWYLTSGAT
                     RTVAGSLETTNALLWGDAVRDLKGDDTDTGVWKRLLPLPFLLPGMNYI"
     gene            10464..12599
                     /locus_tag="P5179_00350"
     CDS             10464..12599
                     /locus_tag="P5179_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRS93702.1"
                     /translation="MSKTDAPPGGAAKSESAPARSLILIGNPNVGKSAIFGHLTGVYV
                     VVSNYPGTTVEVTRGVAQSKEYEIYDTPGANGLVPMSEDELVTRSILLEQPDAPVVQV
                     ADMTNLRRALILTSQLSDWERTGALALNMADEAASRGIHVDLKSVEKLVGVPVRATVA
                     VRRSGVREIAERSFEGRPFQLRIEFQEAIEKAISDITALLPKDMRGKRGVALTVLSGD
                     ETIFPWLKNQTSQEVVQRLEAIREHVRVQFPGGVAAVINTERLRRIDAIVAQVMRIRD
                     TAGNHVLALVSSWATHPLKGLVVVGAVLYVTLWFVGLFGAGTLVDFFETFVFSQFVSP
                     FFIRLADAILPFPHSHVIEPVKWTLSLPLSALHQVDLFTVARSVPSTTYAVTDGVSLT
                     WWQTTARVVHDFFVGPYGMITMALAYGFAIVLPIVATFFIIFGLLEDSGYLPRLAVMT
                     NRLFRMIGLNGKAVLPMVLGFGCDTMATMTARILETKKERVITTLLLALGVPCSAQLG
                     VILAMVATISLTGVLWWVITVAVVMMLVGWLAARVLPGDSSDLVLELPPMRVPQFGNI
                     IVKTIARIEWYLKEVVPLFVLGTALLFFLDLFDALGAIRSFAAPVVQGWLGLPAQATE
                     AFLIGFLRRDYGAAGLLQLQQHGLLDELQIVVSLVTITLFVPCVANVFMIAKERGIRT
                     AIWMVVFIFPFAFLVGGLVRWGMILTGSG"
     gene            12823..13506
                     /locus_tag="P5179_00355"
     CDS             12823..13506
                     /locus_tag="P5179_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRS93703.1"
                     /translation="MGRHEMKHLPRECENILVQVWSAHEKGDKSLGKLFLAHPHAVPV
                     VDELLRAGLLERLDGEIWLTERGAKAARELVRRNRLAEVLLQQILEVQPERVAVNACA
                     WEHVLSAEVTDTICTFLGHPRHCPHGNTIPPGPCCAKSSRTVTPLVQPLTTLPVGGTA
                     RITFIAPRVSRRLDRLAALGVVPGTKLVLKQKSPAYVIRADQTDIAIEEDVAKDIFVR
                     EIKAEEEGG"
     gene            complement(13529..14557)
                     /locus_tag="P5179_00360"
     CDS             complement(13529..14557)
                     /locus_tag="P5179_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93704.1"
                     /translation="MKNIDVSRLVNLGVVPARRFFAATDSLAELTASHGRRTFLAAFP
                     SDRVLSIGLYQDAEIDLEEAATRYPWPMLRRSVGGHEMSVVPGTLLFSMIIPQDDAEN
                     SITRACRGMVPSLVATCRSYGVDAHFTPPDSVRYHRILFSAVYAGCVGNALVLTGTLP
                     CTSEEPGGRNAVEVSLSGISKGAVGPAEVLDVFVAKLGEQCRTEFREERLSPAEEAAI
                     NQKVLAGSESEPRRKPQRAELRERARRDPRLDLREAQFTTAGGRTIGLSLLLRGEVIE
                     SVVFSGDFVFYRDCLSDLETALHGVPAEWDELMTVLENYYIINQVDSPGVTPADWVSA
                     IDAATGML"
     gene            complement(14665..15591)
                     /locus_tag="P5179_00365"
     CDS             complement(14665..15591)
                     /locus_tag="P5179_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS93705.1"
                     /translation="MQPLEFRPDGLFVLVQFTDTHFADLPHNRETFELFDRVLQIEKP
                     DLIVLSGDIVHPAWCKDPLIHWADVVRYFDAKNVPWAFVQGNHDAQEFTYEAIEDTLA
                     GSRLSLYESGPESIFGHGNYAVPIYKRGTSRPGAIVWSLDSGQGAEGVASGWDWVKRD
                     QIAWFSAEADRLMKQDGDSITGLAFVHMPLVQHLTVWQTTPCSGFLNEKVCMQGMDEG
                     LFDAFKERSRVAGCFVGHDHVNDYEGVLEGVTLAYGRGSGYNCYGKEGFRKGARVITL
                     REGVRGFQTHIRLDDGTLAPRPSHLPDGTSLN"
     gene            complement(15723..16013)
                     /locus_tag="P5179_00370"
     CDS             complement(15723..16013)
                     /locus_tag="P5179_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93706.1"
                     /translation="MANKKSDPVPPEEIEITSQGTESAEAEATAPVEQDAQTKTATEQ
                     EAEEQPEGVMAADKDFEENVPCAAFPWKEVVKWSIVVTVVALVLYLIFRRDD"
     gene            complement(16166..>16519)
                     /locus_tag="P5179_00375"
     CDS             complement(16166..>16519)
                     /locus_tag="P5179_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS93707.1"
                     /translation="ELIAPVHAIRGNTDPFDLPFPEVLDTSHKGIRFHVRHVVPTSGR
                     SLEFFTMRTDAHVVLFGHTHVPFLEKVRGTLFLNPGAISRSRVPAETAASSPCGAGKS
                     LRRCTTLSFTNFQCD"
     assembly_gap    16520..16619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16620..17932)
                     /locus_tag="P5179_00380"
     CDS             complement(<16620..17932)
                     /locus_tag="P5179_00380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRS93708.1"
                     /translation="MDWRYERDSLGEVKVPAGALYGAQTQRAIENFPVSGIRFPRSFI
                     RALGLIKYAAADVNEQLGYLDARIADVIRAAALEVAEGKLDEHFPVDVYQTGSGTSTN
                     MNANEVIANRAIQMLGGVIGSKTPIHPNDHVNMSQSSNDVIPTAVHVSAHLKVVEELL
                     PALHHLADTLSHRAEENDDVVKTGRTHLMDAMPIRFGQQLRGYQTQVSKGVKRIEATL
                     PRLAELAIGGTAVGTGINADKAFAGGVAEKLSELTGIRFRETDNHFEAQSAQDAAVEL
                     SGQIRTVAVSLMKIANDFRWMNSGPQAGLGEIVLPAVQPGSSIMPGKVNPVICEATTL
                     ACAQVMGNDLAIAIGGQSGNFELSLMLPLIAHNLLQSISLLANVSRLLADKALTGFRV
                     NARRMTDLVEMNPILVTALAPRIGYDLAAHIAKRAMAEGRKVRDVA"
     gene            complement(17957..19924)
                     /locus_tag="P5179_00385"
     CDS             complement(17957..19924)
                     /locus_tag="P5179_00385"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HRS93709.1"
                     /translation="MPSTTANGVIWQITSNNITVHVDTVTMTLTMQRSGGGPWRMVET
                     SHDLTLALNGHETPVALASADQKQATLYEAGELSGVRVVLRGFPAGNRSVDVSLAIIV
                     AIDGVSDEPVVRVIPAHDPQRVIREVRYPRPFVLEGRGPGVHHVLPSMQGALLPTDWP
                     GKIDWWWIGLNWTRAIYMPWWGAIRPGDAYMGILETPFDGGLHFSHPPGGPTEVGPKW
                     HPSMGSLSYPRQVRYHLFGSVDHNALVAKYREYARTIGRLKTLETKATEVPEVKLLQG
                     TTVATGYTLYHIQPDSHYYHPDDPGKNHNLRTFEESIRGVERLREIYPTDRLVYHLDG
                     WGTRGYDNLHPDYLPPCEEAGGWDGFKKLAEAVDRLGWLFTTHDNYIDYYVDAPTYRE
                     YLARVAEDGSVPGKAWWYGGAQTFLCERNALGFVRRNFEEILRRRVPLRATYLDVFSI
                     IEMLECYHPDHPMTREQSLQARAECFEWVRSRGIVLSSEEPTDWSVPYIDFCYWAPVA
                     MADESPDAPTFGIPVPLFNQTYHDCVVTPAPLGKSGAVSEKDAFLYALLYGCPPIVAA
                     PEPKVQSGPGAPVESAARSAHFHRAKILADVHRQTGFEPIEAHDLLNTEGTARVTRFT
                     GGASIEGDFTAGKYRIVGLKGIDDTWQVVPD"
     gene            20107..21198
                     /locus_tag="P5179_00390"
     CDS             20107..21198
                     /locus_tag="P5179_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93710.1"
                     /translation="MTSRERLRAIFAHEPYDHFGIFEHFWGETIPEWRKQGFPEDGDP
                     ARFFDYDLRGVGGGPNATPFRDFSELIQETDEWKITRSGWGAALKHWKHHSGTPEHID
                     FLIKDATTWRPYRDQLVGPADPTRLDIDGTRKNWKEAAAEEKYCTFSCLFIVETLRHA
                     LGDVHMLESFILDKPWLHDVNRVITDFYIRHFDLLFKEVGVPDGIFIYEDLGFTNGLF
                     ASPKTLEELIFPYYREFVDFFKGKGAQVLLHSCGGVTEAVPMILEAGFDCLQPMEAKA
                     GVDVVALARKFGNRIAFMGNIDVTKLNTNDRAIVKAEVLGKLNALYDLGAGYVFHSDH
                     SIPPDVTFDTYRYAVDLYRDFCATHPCGA"
     gene            21209..21802
                     /locus_tag="P5179_00395"
     CDS             21209..21802
                     /locus_tag="P5179_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS93711.1"
                     /translation="MNGEPEYYDIRQSAFQTWFEKGLPYEDYLARSDPRHAERWRTAE
                     KAIALTAEQRRLLGSFVRQMPVLCLSGVWCGDCVRQGPILHAFEKAAPVVNVRFLDRD
                     EFPDLMDELRICGAKKVPVVVFLTEDFFEVERYGDRTLSVYRRKAERELGPACPVTIG
                     PQDGNEAAVEIQEWLDTFERVQLMLRLSPPLRRRHGD"
     gene            complement(21874..22797)
                     /locus_tag="P5179_00400"
     CDS             complement(21874..22797)
                     /locus_tag="P5179_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HRS93712.1"
                     /translation="MAASELIKANLLHLSYNMWADREDPERPDSYTTARPYLRFDESL
                     WRDILRKMVENGLNMVIIDLGDGIRYESHPEIAVRGAWSPEKLREELKFIRGLGLEPI
                     PKLNFSACHDVWLGPYSRCLSTDTYYAVCADLIEEVIRLFDTPRFFHLGMDEETANHQ
                     RRFEYVVIRQFDLWWHDFLFYVEQVEKAGVRAWIWSDYIWNNREVFLKRMPKSVLQSN
                     WYYDVVFNETVNYVKAYHDLEEHGYDQVPTGSNWSHPDNFGMTVEYCRKIIAPERLKG
                     FLQTPWKPTLEAERKRHVEAVEQVGRALRGL"
     gene            complement(22779..24578)
                     /locus_tag="P5179_00405"
     CDS             complement(22779..24578)
                     /locus_tag="P5179_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS93713.1"
                     /translation="MVAYHVLPAIRTCRNLVVQGAICLLFGVMCGCAPRETAPEQTVD
                     ETKTIYFATHMGEHPGWIPAFAVLARRYEELHPGWKVRLHYQPLDGYQVWAETQFISG
                     RGPDILDFGGSDAFRFGARMGLLLPLTEQLHEESPYAPGKRWKDTFYPIVWKNAEDPI
                     YGELWWVPFNFFTVRIFYNKELFARAGITQPPTSWKEFVAVQQKLLDAGIVPYLMPNS
                     NNNPHKYIFTCMEFLSDMMFQDRVPEIDTVIRDGQVDGIESNISIYKGLTRLDDPQML
                     AVLHLLKEWSRYWVRGYNGLDRQQSKMLFANGIGAMYMDGSWETEGLAEAVNFDWGVF
                     PMPLVTKETTPYADGPRNDGSYNLSFSIGKVAERRGHLAQVVDFLQFLTSPEAADTLR
                     NYVSFISALEDYPVPDDLKSFAPASGYPWSYNAWAVSPQSASMEGGDVLFSLLQGYCA
                     GSISERDLLERYGKTYRELTVEELRFALRENAQRVLEGRRRLTATRAVRDSLARVLGA
                     DPDSTTATCAEFARLNRHVAFQEESLRRANLQFSALLETLSRPPAWERSLHTSGTGTQ
                     PALLSHSPCRSTPDPFRFPTTSVEGAQQWLHQN"
     gene            24742..>25857
                     /gene="apbC"
                     /locus_tag="P5179_00410"
     CDS             24742..>25857
                     /gene="apbC"
                     /locus_tag="P5179_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRS93714.1"
                     /translation="MPEVTKEMVVEALSKVNDPELHRDLVSLGMIRNVAVSDGAVKFT
                     LALTTMACPLKSRMQEDCKTAVMRIPGVREVTIDLTADTPRNARPASGKKVGDIVLQD
                     MLPTVKNAIAVASGKGGVGKSTVSVNIAAALAMEGARVGLLDADIYGPSIPIMMGVTE
                     QPEITDSEEIIPIRKYGIELMSIGFVIPEEQSVIWRGPMVAKMLQQFLSGVKWGELDY
                     LIVDLPPGTGDAQLTLTQSVPLAGGLIVSTPQAVALADVRRGITMFKKVDVPILGVVE
                     NMSTFVCPKCGHEEHIFAYGGARSEAEKLGVPFLGEIPIDLSIREGGDSGVPVVVAHP
                     DSPVGLAFRKLARDAAAAVSVSNLTARKAERHEPARTG"
BASE COUNT         5252 a         7495 c         7517 g         5493 t
ORIGIN      
        1 ttctcaaaag gattccgggt aaaggtcaaa ggcctgcgca aggagaccgc gccactcagt
       61 cccccaggcg gtagtacccg aaactgctgg gatctatgtg ccggagtctt cgctggagtt
      121 ctgttttctg cctctcggca cgctgttcgt cggtttcttc cggttgtcct gcctcggtat
      181 cgctctgctc ggtctccggc gatcgaaaca ggccatcctc cgcccatatt ggtgcgtcaa
      241 aacgcaatgc cagcgcgatc gagtcgctgg gacgtgcatc gatccgcgcc tctcccgcat
      301 ccgtgcgaac aaccagctcg gcgtaatacg tggaatcctc gagctttgtg atttccacac
      361 gcaaaagccg ggcttccagc aaatccatga ccgatttcat cagatcatgt gtcagagggc
      421 ggccgcagtc ctgattctcc agtttcatct ggatggaatg cgcctctgga tgccctatcc
      481 agatcgggag cacacgttcc ccctccgctt ccttcagcag aacgaccggc ccgtggttca
      541 cgtccaaacc gaggccgtgg actaccacct gaaccatcgc catggacgtc tccctgtgcg
      601 ttcttgcgac caattcctac atttttacag gtttgttagt caatataggc cggccggcga
      661 aaccggtcaa taacatccct ttgtgccgca accatctcga cccgcaattc gcgtttctca
      721 accgaatcgc gccccagcag ggagaagccg tgaatcagac ccctcgtcat tccccgtttc
      781 gcgtcgcgtc tgtgcagttc cgaagctctg ggcacgtgga acagaacgtt gcgcgcatcg
      841 ttgatcatct ccgtgatctc tcgcgacaag gcgtctgtgc ggtcgcgttt cccgaatgcg
      901 cggtcaccag ttacgacgag aaggcgatcc gggccaccgc cccggaacgc ctctcgcagg
      961 cggaaaagga gattgccgtt gcgtgcagag agaatggaat cggcgtcgta gtcggcatcc
     1021 cttactggga acaaggcact ctgtacaacg gtgctcttgc gttcgactca ggggggaaat
     1081 gcgtggcgag gtacgccaaa atccagcttg caggggagaa gtggtgcgct gcgggagccc
     1141 gtttcgctat gttcagcttc gattcggttc catgctccgt gatcatttgt cacgatgaac
     1201 ggtacccgga actggttcgg ctacccgtgc ttgcgggcgc gcagatcatt ttctacatct
     1261 cctgcgagtc tgacatcaca gcggaaacga aaatcgcccc gtaccgtgcc cagatccagg
     1321 cccgagcgat ggaaaacagc gtcttcgtgg tccacgcgaa cgctcccatg gggaatgttc
     1381 ggatgcgaaa cggaaaaccg cgtggggaag cggccgcgtc caacgggcac agcaggatca
     1441 tcaaaccgga tgggaacatc gtcgccgagg catccatctg gggggaggat gtcctggtcg
     1501 ccgatctgga tatgtccacg gcgacgcggc accttgcgtt gcgcagcctc gaatcccctc
     1561 tcctgcattc gtggtgggag gcgggtctgc gcctcgttcc acctccaacc cggtaagcca
     1621 cctgaggcct aacaggatca acatccctgt ccatcatgaa ggtctccggg ctccgttttc
     1681 catggaacgc tggttcgctt ttcgtatctt tgaagagacc tggttccacg ggttctcgag
     1741 aaagggtgcg acgcaatgag ggtcagtccg caatctatcc tccttgccgc cgtgtttctc
     1801 tttgcgtcct ccggatcgca tgcagaggtg caaagcccga atgaagcccg tgcgagcatc
     1861 cccgcagact gcgaagcagg tcgcgtttgt gcgatgaacg acggggtact cgatccagtt
     1921 ctggcaagcc ttctttccga tatgggagga ctgccgccgc gagcgcggat tcgcgtgttc
     1981 acacccgcag acatcatcgc cgcgacgaat ttcgatccgc ttacgatttc caccgctgcg
     2041 gtgacgtgga tcgtgatcgc tcaaacaggc gaacgtgggc cggtgcactg ggcgagagcc
     2101 tcaataacac cgcaagggat catggtcaag agaggggtct tttttccgtc gcgcaaggcg
     2161 tacgcgtact ccctggcacg gagcgtggcc gtacggttcg ggatttgcag aggggaagac
     2221 cagtcgactg gagcaagttt cccggtattc atgggaaacc ttccttgagg caccgcggat
     2281 cccggtcaaa ttgggttcag gatcgttaat agccgggaac gacagcgaaa ggggcactgt
     2341 catgacaaat aatgaagtag tggaattgct tgcccacgac atgagcatgg agcatcaggc
     2401 gatacttcaa tacctgtttg cagcatgggt tatcggcggg gaggtcgggc cctcgatcga
     2461 gagcgttgcc agggacgaaa tgcgccattt caagtacttt ggcctggcca ttgccgaact
     2521 cgggggaaaa ccgaatatgg agggacccgc cttccagcgc ctcaccgatc ccacggccat
     2581 tgttctcgcc aatattgcct ccgaagatga ggcagtcaga gtttacacgc agcaccggga
     2641 tctgatcccg gacccgcgga tcaaaaaact gtatgacagg atcatcgaag aggagatgta
     2701 ccacggtcag aaattccagc gcatggtgcc caaagtcgaa gggatgccgc ccgtggagtt
     2761 ccacgagcct cagtctccgc aacaggagac cctcttccga tttctcgaag aggatattgc
     2821 cggcgagtac gcggcgattc tgcggtacct gcaccagtcc ttcgtggtgg cggacggcaa
     2881 actgggaaac gcaattgaag accgcgccat cgatgagatg aagcacatgg gctggatggc
     2941 ggaacacaaa atcgactccg gcggagagcc ggtgttgcag ccggcaccag ttcagtactc
     3001 gacggagctt tcggagatat tccagtacaa cattcagctt gaaaagggcg ccgaggaacg
     3061 gtatcaacgg cacattgacg ggtacgaaga tcctgacctt cagagggtgt gggcgcacat
     3121 caagttccag gaagagcacc acactgacga ctttgaaaac cggcttgcag acctcaacag
     3181 agcgccggaa gcccaggcgg ccacaccacc ggatgcttcg aacgtgaagc caaaaacctc
     3241 cgtcggatcg ttgtttggca aaccgcagtc gtgattttgc gcgagtaagt cttttcatac
     3301 gtgaacagaa ctcgaaacaa ggggaaggaa aatggcggac taccttttgc gtgagaacgc
     3361 tccgctcaca gagcaggaat gggccaaagt agacgaactc gtcaccagcg tggcaagccg
     3421 attgctcgtc ggtcgtcggg tcctgcctct gttcggccct ctcggggccg gcgtgcagac
     3481 ggttccggta gacacattcg acctcgcctc cgaagaaggg atccggagat cgggacgagt
     3541 gtttgtagtg cctcgcatga tagcgaggga attcatcctc gggtggcagg acatcgaaac
     3601 cgggcgcagc ggtgttcttc cgctggaact gagccccgcg gctgccgcgg cttcggcctg
     3661 cgcactggcc gaagacacgc tcatctttag aggtgatgcg gaatccgacg ggattctgaa
     3721 cgcagagggc tgccttggaa cgaagatgag tgactgggcc accgaaggcg cgtttaacga
     3781 catcctcgct gcggtccaga agcttcagga gaatggcatc actgagaaac cagcgcttgt
     3841 cgtaagcccg gcaggctatg cgcttatgca gaagccgttt gggaacacgg gagcattgga
     3901 acttgcgttc gtccgtgagc tcatgcccgc cggcgtgttc cagacacccg tcctcaaacc
     3961 gtcggaagcc gtggtaatcg ccaccggtcc gcagaatgcc gatcttgcgg ttggcgttga
     4021 cctttcggtg gggtacgtag accaggtcga catggaccat cgcttccgcg tactggaaac
     4081 gctggcgctg cgggtgaaac ggccccaggc aatctgcacc atcacccggt aacgggcggc
     4141 gggaagccct gcgtgcgccg cgagacggaa acgacgcgtt ggatccgcac cgtgcgggag
     4201 gagattcccg cacggcgcgg ttcttttctg gtcgcatggg tagacaagtc ttcgcaacgg
     4261 gaatcgccgg tggtgatcgt cggcaggacc gtcggaaagg ctcacgaacg gaatcgagta
     4321 cgccgcagga ttcgcgaagc cctgcgtgcg tgtgtggcga atccggccgg catcatcgtt
     4381 gttgcacgaa ccagagcccg cgccgcagat tactggaact tgctgaatga gctggagggt
     4441 cttcttgccg aatcggatgc tcgtatgggc ggcgatcgga gtgattagga tctaccagtc
     4501 cctgttttcc ccccttctgg gagcttcctg caggttcttt ccaacgtgct cgtcatacgg
     4561 tatcgaagcc ctctcccgtc acggattctt ccgtggcggg tggctcacac tcaaacgaat
     4621 tttgcgttgt cacccgtacc acccgggcgg gtacgacccc gtaccgtgag aggaattgct
     4681 gatcgtggaa cgtaatactg ttatagcatt ggtgcttatc atcctcatct ggatcctttg
     4741 gccaaagtgg atcaaatgga cctcgccgga gacacagccc gtcgttcctg cagcactgcc
     4801 tgcggattca tctcgggggc cgagttcgat tccggactcg atcgtccggt ctgcaccacg
     4861 tccggtggtt gctgcctccg atacaacccg ggtgcgcgcc atcgctcacg cagattccgt
     4921 acccgtccgg acctgcacca tcatcactga caaatacgag gccgttcttt ccaacgaagg
     4981 ggccgtgctg cggaagtggt cattgaagga ttaccacacc ccgtacggtg cgcctgtgca
     5041 gttggtggct ccgggcggac acggtatttc gttgtcagtg ggttccgaag ggaaggaaat
     5101 cgacctgcgg gagaaggttt tcgttcccga tacggtgttc cagatggcgc atctgactgg
     5161 caaggacagc cttgccgttg ggttcacctg cacgcttccg aacgggcggc gtgttttcaa
     5221 acgctatgtt ttttatgggg ataggtaccg agttgcattt gcggcgggct ccgcggattc
     5281 cttgaccgcg gaacggatgg ttctatcctg ggaaggtgcc atgccttcga cggagggtga
     5341 tttccgacga gaggcaggga gcatgttcgt gaacgcctac gtcggcggat ccctggaaaa
     5401 actccacacg ctcgacaaga cggaaacctc gtcgtttacg gggcaaaccg attgggtggg
     5461 tgttcgaaac aagtatttcc tcgcggcttt tgcgccgaca attacggaca ggtgggacac
     5521 aaggcttttc ggaagtggcg tcaaggacac gagcctgcgc tactcctggc gcatcgcacc
     5581 cttcgatttg acaaaaagca cggtaaccgg attcctgtac ctggggccgc tcgatgttga
     5641 catactcacc gccgaggggc acaatctgga acgcgccgct gacctcggct ggagcgttct
     5701 ccgccctatt tccaaactta tcctctggtt cttcctttcc gcctacaggt tcatacccaa
     5761 ttacgggtgg gtaatcgtcg ttttctctgt tctgatcaag attcttctgc atccgttgac
     5821 gaaaaagagc tacgagagca cgagcaaaat gcagaagctg aagccgctga tggacgaaat
     5881 ccgcgaaaaa tacaagaacg atcagcagcg gttgaaccag gaaatgatca agctgtacaa
     5941 ggagcacggg ttcaacccgc tgggcggatg cctgccaatg ctcctccaga tgcccatcat
     6001 cttcgcaatc tatgcggtga taggcaacaa cattgaattc aggcaggccc cgttcatctg
     6061 gtgggttacc gacctttcca tcccggattc tgtgtatacc ttgccattcc atatcccgct
     6121 ttacggcagt catgtgacaa tactgccaat tctgatggcc atttcgatgt tcttccagca
     6181 gaagctgacc gtcacggatc ccaagcagca ggcgctggtg tacatgatgc ccgtcatcat
     6241 gctgtttgtg tttaataatc tcgcctcggg acttgtgttg tactggttcc tgttcaacat
     6301 cttctcgtcg gcgcatcagt actggctgat gaagaaacag aacggaggcg aaatcactcc
     6361 gaaaacggcg ccggttccgg tgaaaaacgt ggtcagaccc cgcaaacact agctcgaacg
     6421 tggttccggc gctcggcgca ccgccgggaa ccacattcca tgacagaccg tttgccatga
     6481 ccgacacgat tgttgctctc gcaacccctt ccggtgaagg cggcatcgcc atcgtcaggt
     6541 tgagcggcac tcgggcgatt tcgatagctg actccctttt tcgtgccccg cggcgccttg
     6601 cagatgcccc tacccatact ctcctctatg gcaggcttgc actgaacgac tccgctgcat
     6661 caccgggcag cggcgtgttc gatgaggttc tcgtttccgt aatgcgcgca cctcggagct
     6721 atacgcggga agacgtggtc gaaatcaact gccacggagg aattcgactt gcggacgaag
     6781 tgattcgggc gtgtgtcagc ctcggcgctc gccgcgcgga gagaggcgaa ttcacggaac
     6841 gcgcgttcct caacgggcgc ctcgaccttg cgcaggcaga agccgttctg gacctcgtga
     6901 aggcgcgcac caaagacgcg ttgtctgcgg cgtattacga actcaacggc ggtctttccg
     6961 cccggatcaa tgccctgcgc gaccgtttga ccgcttcgct cgcgagaatc gaaacgggac
     7021 ttgaatttga cgaagaggac ctgggcgtag gcgatccacg cgctgaaagc gccgcactcc
     7081 gtgaaatcct ttcggaaatc agcgctctcg ttggcaccta tgcccggggc agactcattg
     7141 cagaaggcgc gagcgtcgcc atcattggcc cgcccaatgt gggaaaatcc agcctcttga
     7201 atgcgatact cggggaggac cgggctattg tatcacatat cccgggtacc acacgcgatc
     7261 tggtcgaagg cgaagccgac ctcgatagtg ttcgggtccg atttgtagat accgcggggt
     7321 tgagggaaac cggggacgcc gtcgaaaggg aaggcacgaa gagggccgga agggccgctt
     7381 ccgctgccga cgtcgtgctg ctggtatgcg acgggtcagg cgaaataccg tgggacctca
     7441 cgcccgtttc gggcagaaac gccatcatcg tcataaacaa gatggacaaa gcgcgcaacg
     7501 aggtgttgtc cgtgctcagt gcttccgcgg gaagcacacc ggtcttccgt gtcagcgcac
     7561 tgtccggtga aggcctggat accctttgcc gtgggattcg cgatgctctg tgcgcggatg
     7621 ccccggagcc gaaaacaggg ggagtcatca tccgtgcgcg acatgcggac gctctttccg
     7681 cagccgggag ccatctggaa cgcgcccttg acgaggccac cggtcaagcg cgctccgaac
     7741 tggtcgcagc ggacattcgc ctggccctgg atgctctggg tagtatcacg ggggagacaa
     7801 cacccgaaga catcatccgc cgcatcttct cggagttctg cgtcggcaaa taggtgtccg
     7861 ggagcgttgc gcggcgtatt cctcttgtca gcgaaccatc acgcttcctg ctcctgctgc
     7921 cacggcattc ctgatccgtt caattgcgtc ctcccgccgt ttcgtgatat cgaaacggga
     7981 caggcgcaac gcggtccagc catcgggtgt ttcggcgtgc ctccccgccc ttttgggaag
     8041 gaacccattc tgattctccc ctcgcgcgac gtccacatgc acgctcccct tgcggccgag
     8101 gatcgcgaaa tcagtgaagt agtacgcgtt gctgcctcgt acgtggtatg ctctctcggg
     8161 cctcaacccc tcctcagtca tcctgccgta gaacgagtcg gtcgcctcgt ctcccacggc
     8221 aagttcgttg atatctcctg ccagcatgaa acgctcaaac gtcgtcggaa tcagcaccac
     8281 ctgtcgtttt cttttgctgc gaatgggtga cgggcgctgt tgcaggtcat cgatgtgaac
     8341 gcgatagtag tccgccctgg cacgggggtg cttgggctca tccggaaaca gatccaccct
     8401 cttgacgatg tctacgtaac ggatgggcgc aaaatacctc acctggcctg cttccacgcc
     8461 gaacccctgc ccatgataga acgcgaggta cgccggcgta gcccactgcg acacgaccga
     8521 acagggtatt cggtaccagt gcttcttcgt gacgatctcg aagtcgctca cgcgtgggac
     8581 aacactcaca aggaccgtgt ccttctccgg gggcaacagg gggtcgcgcc tcaggagatc
     8641 gtcaaacgtg cgggcgttgg agaaacgcca ccacgtggtc atggtccaga caaaacgccg
     8701 gggtcgtgcg cattgaagcg ggtgagccag ccgtgcggga ggatcgaacg agtacaccag
     8761 atacggttcc tgcgcacggg ggtggtctgt ctccgaggga aacagctcga ttctccgcac
     8821 cgtgtgcgtt cctcgaacac gtgcgtagga gtacacaccc catcgctccc tgccaaaacc
     8881 cgcaggctgg tagaacgcaa tccattcggc gcccgggtcc ttcggagcat gcgcagccgg
     8941 aatacggtac cagttgcggt ctcgagcgat gtcccagtcg gtggtgcttt tcaggagcac
     9001 cacgagcacg cgttcgtgtg gttccatccg ccccgatcgt ccagatgcag cttcagatgt
     9061 agttcattcc gggtagcagg aacggcaacg gcaacagccg tttccacact ccggtgtccg
     9121 tgtcgtcccc tttcagatcc ctcaccgcat cgccccagag caacgcattc gtggtttcca
     9181 acgaacccgc aacggttctg gtggcgccgg acgtcagata ccagacatcg tccgactgga
     9241 caaggttcag ttcgcccacg aactccgtac ccagtcgttc cgtcacccac aggtcgtacc
     9301 gacgaagaac ctccggattc agaaaagaca tccgatggcc gccgatgctg gatgcctgca
     9361 cggatccgcc gagaccggtg agcaaaccgc tcatctcgcg gtcggaaggg aggcagctcc
     9421 cactaaccgt ccgcagcccg aaccgctctt tcagcaaggc gagcccggcc agcactgctg
     9481 cacggtttcc ggcaaactcc tggacatacg cggtcgggtt ggtgttgtct cgtcccacgg
     9541 aaagaacgaa atacgcaacc ggcgtgccct gctggcggac aacccagaga cggcgaaccc
     9601 cgaacggagg ttccgctcct tttggggtga gacggtacac cgacaggaac tgctgccact
     9661 cccagggtga ccggacaaac cgcgaaggtt cgttctgatg cagcgcgatc aggtcggaaa
     9721 tgtccgcatc cgcagcttcc tcgacgacca atcctctttc cggtgccccg gccagtgccg
     9781 atccatcgag cgagaaacgc atctcatgtc ccgcgatggc gcaccccgct ctctgataca
     9841 atccccgttg tcccgagatc aggacaacgt caacgttctc acgtttcatc tgcgcgaacg
     9901 tgtcttcaag gagggcggtc gccagcccgt gtccccggta ctcctcgaag gtcgcaacag
     9961 cacccagcga gccgattctc agcgtgttcc cttgaatgac tgccgttctg ctgacgtagg
    10021 caatggccga aacgggttgc cccttgtcga cgaaaacacg caacctctcg caattggatt
    10081 cgtggaagag cgtggggaat tctcggaaca tgcagctttc gccgttcgag cggaagacgg
    10141 tgttttcgag agtacggagg ccatcgagct cttccgggcg acacgcgcgc ggaccttcca
    10201 tgggcacctc gatctatccg ggtgtggtgt gcaggcaggg cttcccttcc ctttttcagg
    10261 tttgaattta gatgcgtttg cgattgtacg ccagccacac ctctccgcgg catggcgcat
    10321 gccgcgttct ttctgagacc ggagggcgtg aagtccaaac ggaattgaga atgaatctca
    10381 attaagttat attccagtac gaagcgatgc gaaccgcttc ctatccactc accatcctgc
    10441 actcgtctcc cgaaggatgt gaaatgagca agacggacgc tccccctggc ggggccgcaa
    10501 aaagcgaatc tgctcctgct cgctccctca tcctcatcgg caaccccaac gtcggcaaaa
    10561 gcgccatatt cggtcacttg acgggagtgt acgtcgtcgt atccaactac cccggcacca
    10621 ccgtcgaggt caccagggga gttgcgcagt cgaaggaata cgagatctac gacacaccgg
    10681 gcgcaaacgg gcttgtgccg atgtccgaag acgaactggt cacgcgctcg atccttctcg
    10741 aacagcccga tgcgccggtt gtgcaggtcg ccgacatgac aaaccttcgg cgggcgctca
    10801 ttctgacgtc acagctttcg gactgggaga gaaccggtgc gcttgcgctc aacatggccg
    10861 atgaggcggc cagcagagga atccatgttg acctcaaatc cgtggaaaaa ctggtgggag
    10921 tccccgtccg ggccacggtc gcggtgaggc ggagcggcgt tcgtgaaatt gcagagcgga
    10981 gtttcgaggg gcgtcctttc cagctcagga tcgaattcca ggaggcgatt gaaaaggcga
    11041 tttctgacat caccgccctg cttccgaaag acatgcgggg caaacgtggc gtcgcactga
    11101 cggttctctc cggggatgag accatttttc cgtggctgaa aaaccagacc tcccaggagg
    11161 ttgttcagcg ccttgaagcc attcgggaac atgtccgtgt ccagtttccc ggcggcgtag
    11221 ctgcggtcat caacaccgaa cgattgagaa gaatcgacgc catcgtcgcg caggtgatgc
    11281 ggattcgtga tactgccggc aaccatgttc tcgccctggt ctcctcatgg gcgacgcatc
    11341 cgctgaaagg gctggtcgtc gtcggagcag ttctgtacgt gactctctgg tttgtagggc
    11401 tgttcggcgc gggcaccttg gtggatttct tcgaaacgtt cgtattctcg cagttcgtca
    11461 gtccgttttt catccgtctg gcggatgcca tcctgccgtt ccctcactcg catgtcatcg
    11521 aaccggtgaa gtggaccctg tccctcccgt tgtccgcgct tcatcaagtg gacttgttca
    11581 ccgtagcgag gagtgttccg tctaccacgt acgccgttac agacggcgtg tctctcacct
    11641 ggtggcagac gacggcgcgc gtcgtgcatg acttctttgt cgggccgtac ggcatgatca
    11701 ctatggccct cgcctacggg ttcgcgattg ttctgcccat cgtggcaacg tttttcatca
    11761 tattcggtct tctcgaagac tcagggtacc tcccgcgtct cgccgtgatg acaaatcgcc
    11821 tgtttcgcat gatagggctg aacggtaagg ccgtgcttcc catggttctg ggtttcggat
    11881 gcgatacgat ggccaccatg acggcgcgga ttcttgaaac gaagaaggaa cgcgtcatta
    11941 ctacgctcct ccttgccctg ggagtaccct gttcggcgca actcggagtg attctcgcga
    12001 tggtggcgac catcagcctg acgggggtat tgtggtgggt catcacggtt gccgtcgtca
    12061 tgatgctcgt tggctggctg gcggccagag ttctgccggg tgactccagt gatctcgtgt
    12121 tggaactccc gcccatgcgc gtaccgcagt ttgggaacat catcgtgaag acgattgcca
    12181 ggattgaatg gtatctcaaa gaggtggtgc ctttgttcgt tctcggtacc gcacttctgt
    12241 tcttcctgga tttgtttgat gctctgggtg cgatccgaag tttcgctgcg ccagttgttc
    12301 aaggctggct gggcctccct gctcaggcga ccgaggcatt tttgataggc tttctgagaa
    12361 gggattacgg cgccgcggga ttgctccaac ttcaacagca cggcttgctg gatgagttgc
    12421 agatcgtggt cagcctggtc actatcaccc tgttcgtgcc gtgtgtggcc aacgtgttca
    12481 tgattgccaa ggaacgaggc attcgaacgg cgatctggat ggtggtgttc atcttcccgt
    12541 tcgcgtttct ggtcggtggc ctggttcggt ggggtatgat ccttaccggt tccgggtgag
    12601 ggcgctgaac cttctgcgtg ttctgggtgt ttgattttgt gggggcgtga cggagtgcga
    12661 gggaacaatg atcgtctgtc ctttgtgcag taaggaaatc ccacaggatg tggagcagtg
    12721 caggacccgc tgccccatgg gaaagaattg cggcctgatc cggtgtcctt attgcgggta
    12781 cgaaatgccg ccagaatcac gcctcgtcaa gcttgtcagg aaatgggtag gcacgaaatg
    12841 aaacacctgc cacgagaatg cgagaacatc ctggtgcagg tctggtcagc ccacgagaaa
    12901 ggcgacaaat ctctgggcaa actgtttctt gcccatcctc acgcggtccc ggtcgtcgat
    12961 gaactcctca gagcagggct gctggaacgg ctcgacggcg agatatggct gacggaacgc
    13021 ggagcgaaag ccgcacgcga acttgtgcgc cgcaaccgcc ttgcggaggt gttgctccag
    13081 caaatccttg aagtgcaacc cgaacgagtt gccgtgaatg cgtgcgcgtg ggagcatgtg
    13141 ctttcggcgg aagtcaccga cacgatatgc acgttcctgg ggcacccgcg gcactgcccc
    13201 catgggaata ccatcccccc ggggccttgc tgcgcgaaga gttctcgaac cgtgacaccc
    13261 ctggttcaac ccctgacaac gctccccgtg ggaggcaccg cgagaatcac gttcatcgcg
    13321 cccagagtca gcagaaggct tgatagactc gcagcgttgg gcgtagtgcc gggaacaaaa
    13381 ctggttctga aacagaagtc tcccgcgtac gttattcggg cggatcagac ggatatcgcc
    13441 attgaagaag acgtagcgaa agatatcttc gtgcgagaaa taaaagcgga ggaagaaggg
    13501 ggatgatgac cggggaatgc gcacactcct agagcatccc cgtggccgcg tcgatggccg
    13561 acacccagtc cgcaggagta acaccggggc tgtccacctg attgatgatg taatagttct
    13621 ccagaacagt catgagttcg tcccattcgg ccggcacgcc gtgtaacgcc gtttcgagat
    13681 cgctcagaca gtcccggtag aacacgaaat cccccgagaa tacgacgctc tcgattacct
    13741 ccccgcggag cagaagactc aggccgatag tgcggccccc ggccgtggtg aactgggcct
    13801 ctcgcaggtc tagccgggga tctcttcgcg cgcgttccct gagttccgcc ctttgaggct
    13861 ttcttcgggg ctcgctttcg ctgcccgcaa gaactttctg attgatggcg gcctcttcgg
    13921 cgggagaaag acgttcctcc ctgaattccg tgcggcactg ctcgcccagc ttcgcgacaa
    13981 atacgtcaag aacttccgcc ggtccaactg cacccttcga tattccggag agcgagactt
    14041 ccacggcgtt gcgaccgccc ggttcttcgc ttgtacacgg gagagttccg gtgagaacca
    14101 gtgcgttccc aacacaccct gcatacactg ccgaaaaaag aattcggtgg tacctcactg
    14161 aatcaggtgg ggtgaagtgt gcatccacgc catacgatcg acacgtcgcc acgagagaag
    14221 gaaccatgcc tctgcacgcg cgggtgatgg agttttccgc gtcgtcctgc gggataatca
    14281 tcgagaacag cagcgtcccg ggaactacgc tcatctcgtg cccgcctacc gaacggcgaa
    14341 gcatcggcca tgggtatcga gttgccgcct cctccagatc gatttcggcg tcctgataca
    14401 ggccgatact gagaactcgg tcactcggga acgccgccag aaacgtgcga cgcccgtggg
    14461 acgcggtcaa ctcggcaagg gaatcagttg cggcgaagaa acgccgcgcg ggaacgaccc
    14521 cgaggttcac caatcggctg acatcaatgt tcttcatgca gcgcccctgc tgaaaaatcg
    14581 ccccgcgaga tagcgctttg cgcctctgtg caaccttgga cgcacacacg aacgcggcga
    14641 cacaactacc gaaccctgcg cccctcagtt gagggaagtg ccgtccggca ggtgcgaggg
    14701 acgcggcgcc agtgtgccgt cgtccagcct gatgtgggtc tggaacccgc ggacaccctc
    14761 acgtagcgtg attacccgcg cgcccttccg gaatccttcc ttaccgtaac agttgtaccc
    14821 cgaaccgcgg ccgtacgcaa gtgttacgcc ctcgagtacg ccctcgtaat cgttgacatg
    14881 atcgtgcccc acgaaacacc cggccacgcg gctcctttcc ttgaaggcat cgaaaaggcc
    14941 ctcatccatc ccctgcatgc acaccttttc gttgagaaaa cctgagcacg gtgttgtctg
    15001 ccagacggtc aggtgttgca ccagtggcat gtgcacgaac gccaatcctg tgatggagtc
    15061 cccgtcctgt ttcatgaggc gatcagcttc cgcggagaac cacgcgatct gatccctttt
    15121 cacccagtcc cacccggatg caactccttc tgcgccctgc cccgaatcga ggctccatac
    15181 aatcgcgcca gggcgcgagg ttcctctttt gtagatcgga acggcgtaat tgccgtgccc
    15241 gaagatgctt tcggggccgg actcgtaaag gctcagtctg ctgccggcca gtgtgtcctc
    15301 gatcgcttcg taggtgaatt cctgcgcgtc gtggtttccc tgaacgaaag cccatggcac
    15361 gttcttcgca tcgaagtacc tgaccacgtc agcccagtga atgagtgggt ctttacacca
    15421 cgccggatga acaatatcgc ccgaaagaac gataaggtcc ggtttttcta tctggagaac
    15481 ccggtcgaaa agttcgaacg tttccctgtt atggggtaag tcagcaaagt gcgtgtccgt
    15541 gaactgaacc agcacaaaaa gcccgtcggg acggaattcc agtggttgca tacagtgccc
    15601 tgattcgcgg gtttccggtt acctggggcg gatatgagcg tctgacgaaa cgcgccaccg
    15661 gatgcgctcc gaaaatggtc tgcttcccgc cctgagaaga gggtgattgc ttattagacg
    15721 gctcagtcgt cgcgtcggaa aatcaggtag agcaccagtg cgaccaccgt gacgacgatg
    15781 ctccatttga ctacttcctt ccacgggaac gcagcgcacg gaacattttc ctcgaaatct
    15841 ttgtccgccg ccatcacgcc ttccggttgt tcttcggcct cttgctcggt tgcagtcttc
    15901 gtttgggcgt cttgttcaac cggggccgtc gcctcagcct cagccgattc cgtgccctgc
    15961 gaggtgattt cgatttcttc cggtgggaca ggatcgctct tcttgtttgc catcggtctg
    16021 ctccttgcgt gaaatggctt acttctgagc acgaatgacg cgccgtgcaa cgtgctcccg
    16081 ccttaacaac ctaaccccgc cgaacctgat tggcaagtga acgacgtcac gccatggcat
    16141 cacgcgcccg cttctgccgg gtacctcaat cgcactggaa attcgtgaac gacaaggtcg
    16201 tgcaccgtcg cagcgatttc cccgccccgc acggtgatga ggcagccgtc tccgcgggaa
    16261 cgcggctcct gctgattgct ccggggttca aaaacagcgt cccgcgtact ttctcgagga
    16321 aaggcacgtg tgtgtgcccg aacagcacca cgtgagcatc cgtgcgcatc gtgaaaaact
    16381 caagcgacct cccgcttgtc ggaacaacgt ggcgcacgtg aaatcggatc cctttgtggc
    16441 tcgtgtcgag cacttccggg aacgggagat cgaatgggtc cgtatttccg cgtatggcat
    16501 ggacgggtgc gatcaactcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    16621 cgacatcgcg cactttgcgc ccttcggcca tcgcgcgttt cgcgatgtgc gcagcgaggt
    16681 catacccgat gcgcggcgcg agggcggtca ccaaaatcgg gttcatttcc acaaggtcag
    16741 tcattcgtct ggcatttacg cggaaaccgg tgagcgcttt gtccgcaagg aggcggctta
    16801 cgttggccag aagggaaatc gactgcaaca ggttgtgagc aatcagcgga agcatcaggc
    16861 tcagttcgaa attccccgac tgcccgccaa tcgcaatggc aagatcgttt cccatgacct
    16921 gggcgcatgc aagggtcgtc gcttcacaga tgaccgggtt cactttcccg ggcatgattg
    16981 atgacccagg ttgaaccgcg ggaagcacga tttccccgag cccggcctga ggcccggaat
    17041 tcatccaccg gaaatcgttt gcgattttca tgagtgacac ggcgacggtt ctgatctgcc
    17101 cggagagctc aacagcggcg tcctgagcag attgggcttc aaagtgattg tccgtctctc
    17161 tgaaccggat gccggtcaat tcgctgagtt tctccgccac acctcccgca aacgccttgt
    17221 ccgcgtttat tccggttcct actgcggtcc cgccgatggc cagctccgcg agccgcggca
    17281 gggtggcttc gattcgtttg actcctttcg acacttgagt ctggtagccg cgaagctgct
    17341 ggccgaaacg gattggcatc gcgtccatca gatgggtccg ccccgttttc accacatcgt
    17401 cgttttcttc ggcgcggtgt gatagcgtat ccgcgaggtg gtgaagggcc ggcaacaact
    17461 cctccaccac tttcagatgg gctgaaacgt gcaccgccgt cggaattacg tcgttcgaag
    17521 actgggacat gtttacgtgg tcattagggt gaatgggtgt cttcgatccg atcactccac
    17581 ccagcatctg gatggcacgg ttcgcgataa cctcgtttgc gttcatgttt gtggaggtgc
    17641 ctgaacctgt ctggtacaca tccacgggaa agtgctcgtc gagcttccct tcggccacct
    17701 caagtgccgc tgcacgtatg acgtccgcaa ttcttgcatc caggtagccg agttgttcat
    17761 tcacgtcggc ggcggcgtat ttgatcaggc ccagcgcgcg aatgaaagat cgagggaagc
    17821 gaatgccact cacggggaag ttctcgattg cgcgttgcgt ctgagccccg tagagtgccc
    17881 cggccggcac cttcacttcc ccgagactgt ctcgctcata tctccaatcc atatcgaccc
    17941 tcgtcagaac gcgatatcaa tcaggtacca cctgccaggt gtcatcgatg cctttcaggc
    18001 cgacaatacg gtatttaccc gctgtgaagt caccctcgat ggaagcgccg ccggtgaatc
    18061 gcgtgacccg tgcagtgcct tcggtgttca gcaggtcgtg tgcttcaata ggttcgaacc
    18121 ccgtctgccg gtgtacatct gcaaggattt tcgcccggtg gaaatgagcg gaacgcgcgg
    18181 ccgattccac cggtgccccg gggcccgatt gtactttcgg ttctggtgcg gctactatcg
    18241 gcgggcatcc gtacagcaag gcatacagga acgcgtcctt ctcagaaact gcacccgatt
    18301 ttccaagcgg cgccggcgtt accacacaat cgtgataggt ctggttgaag agcggtaccg
    18361 ggatgccgaa cgtgggggca tcgggagact cgtctgccat ggcgaccggc gcccagtagc
    18421 agaaatcgat gtagggcaca gaccaatcag tgggctcctc cgaactgagc acgatgcccc
    18481 gggaacgcac ccactcgaag cactccgcgc gtgcttgaag actttgttcg cgcgtcatcg
    18541 ggtggtccgg gtggtagcac tccagcatct caatgataga gaacacatcg aggtaggttg
    18601 cgcgcagcgg tactcgcctc cggaggatct cctcgaaatt cctccgaacg aatcccagtg
    18661 cattcctttc gcaaagaaac gtctgtgcgc cgccgtacca ccatgctttt ccgggcacac
    18721 tcccatcttc agcaacgcgc gcgaggtatt cgcggtaagt cggtgcgtcc acgtagtaat
    18781 cgatatagtt gtcgtgcgtg gtgaacaacc agcccagacg gtcaacagct tcggcgagct
    18841 tcttgaaccc atcccacccg cctgcttctt cgcacggtgg gaggtaatca ggatggaggt
    18901 tgtcgtaccc ccgcgtcccc cagccatcga ggtggtacac gagtctgtcc gtgggatata
    18961 tctcacgaag ccgctcaact cccctgatgc tttcctcaaa tgtgcgcagg ttatggttct
    19021 tcccggggtc atcagggtga tagtagtggg aatccggctg gatgtgatac aacgtataac
    19081 cggttgcgac ggtagtacct tgcagcaatt tcacctcggg gacttccgtg gccttcgtct
    19141 cgagggtctt cagccgtccg attgtccttg cgtattcccg gtacttcgcc acaagtgcgt
    19201 tgtggtccac gctgccgaaa aggtggtacc gaacctgacg cggatatgac agactgccca
    19261 tgctcggatg ccacttgggt ccgacttccg ttggcccgcc gggggggtga gagaaatgca
    19321 atccgccgtc gaaaggcgtc tccagtatgc ccatgtaggc atcgccgggc cgaattgccc
    19381 cccaccatgg catgtaaatg gctcgcgtcc agttcaaccc gatccaccac caatctattt
    19441 tgcccggcca gtcagttggc agaagcgcgc cctgcatcga gggcagtaca tggtgaacac
    19501 cggggccccg gccctcaaga acaaaggggc gcggatagcg cacctcgcgg attactcgtt
    19561 gaggatcgtg cgccggaatc accctcacga cgggttcatc cgatacgccg tctatcgcca
    19621 cgataatcgc gagagacacg tccactgaac ggttaccggc aggaaaccct cgcaacacga
    19681 ccctgacgcc tgataactca ccagcttcat agagtgtggc ttgcttctgg tccgcggaag
    19741 ccagcgcaac cggtgtttcg tgcccgttga gtgcgagcgt caggtcgtga ctggtttcaa
    19801 ccatgcgcca cgggcctcca cccgatcgct gcatggtcaa cgtcattgtg acggtgtcaa
    19861 cgtggacggt tatgttgttc gatgtgatct gccagatgac gccattcgcg gttgttgacg
    19921 gcatggtatg ttctccggat cagaacggca accccgaggt cggagtcggg tggacaaggt
    19981 gtcgagaagg taccgtgaaa cgcgccgcac cacaaccgca cgatcgccga cgttggggcg
    20041 ccaccgttcc cggcgtagaa ttacctgtgt ggcgacgtgt cactactttg gaaggggagt
    20101 tgcagaatga cctctcgcga gcgtcttaga gcgattttcg ctcacgaacc ctacgaccat
    20161 ttcggaatct ttgagcactt ctggggcgaa accattccgg aatggaggaa gcaagggttc
    20221 ccggaagacg gggatccggc gcggtttttc gactacgacc tgcggggtgt gggcggcggc
    20281 ccgaacgcga cacccttccg ggatttttcg gaactcatcc aggaaaccga tgaatggaag
    20341 atcacgcgca gcggatgggg tgcggcactg aagcactgga agcatcattc cggaacgccg
    20401 gagcacattg attttcttat caaggacgcc acaacgtgga gaccgtaccg cgatcaactg
    20461 gtgggtcctg ctgacccgac acggcttgat attgatggaa cccgtaagaa ctggaaagaa
    20521 gcggctgcag aggagaaata ctgcaccttc agttgtcttt tcatcgtgga gacactgcgc
    20581 cacgcgctcg gtgatgtcca catgctggaa agctttattc tcgacaaacc ctggctgcac
    20641 gacgtgaaca gagtaattac ggacttctac atccggcatt ttgacctgct tttcaaggag
    20701 gttggtgtgc cggacgggat attcatctac gaggacctgg ggttcacgaa cgggctgttt
    20761 gcttcaccaa aaacccttga ggaactcatt ttcccgtatt accgggagtt cgtggacttc
    20821 ttcaagggca aaggcgcgca ggttctgctc cattcatgtg gcggtgtcac cgaagcagtt
    20881 ccgatgattc tggaagcagg gttcgactgc ctccagccca tggaggccaa ggccggcgta
    20941 gatgttgtag cgctggcacg gaaattcggc aatcgaattg cctttatggg caacattgac
    21001 gtgacaaaac tgaatacgaa cgatcgcgcg atcgtgaagg cggaggttct tggcaaactc
    21061 aacgcgttat acgacctggg cgcaggttat gtgttccatt ctgaccattc gattccaccc
    21121 gatgtgacgt tcgacacgta ccggtacgcg gttgacctgt accgggattt ctgcgccaca
    21181 cacccttgcg gagcatgagg tacgaaccat gaacggagaa cccgaatact acgatatccg
    21241 gcagtcagcc tttcagacct ggtttgaaaa gggactgccc tacgaggact acctcgcccg
    21301 cagcgacccc agacatgcgg aacgctggcg gaccgctgag aaggcgatcg cgttgaccgc
    21361 ggaacagcgc aggctgttgg gctccttcgt ccgccagatg ccggtactgt gcctgtcggg
    21421 cgtgtggtgt ggcgattgtg ttcgtcaggg gccgattttg cacgcgtttg agaaggcagc
    21481 gccggttgtg aatgttcgct tccttgaccg ggatgagttc ccggatctca tggacgagtt
    21541 gcgcatatgc ggggcaaaaa aggttcctgt ggtcgtcttt ctgacggagg atttctttga
    21601 agtcgaacgg tacggggaca ggacgttgtc cgtgtatcgc agaaaagcgg agcgcgagct
    21661 cggccccgcc tgcccggtaa ccattggccc tcaggacgga aatgaggctg ccgtggagat
    21721 tcaagagtgg cttgacacat tcgagcgagt tcagctcatg ttgcggctct cgccgccgtt
    21781 gcggcgcaga cacggtgatt gaaccgcttg gtttgaaaaa gaagggggga ggcgtttcgc
    21841 gctcccccgg ggtaacgttg tcctgttccg acattacagc cctctcaatg ctcgcccgac
    21901 ctgctcaaca gcctccacat gccgctttct ctccgcttcg agggtgggct tccacggggt
    21961 ctgcaggaat cccttcagac gctcgggcgc gattattttg cgacagtact caaccgtcat
    22021 gccgaagtta tcgggatgcg accagttgct tcccgttgga acctggtcat agccgtgctc
    22081 ttccagatca tggtatgcct ttacgtagtt caccgtctcg ttgaacacta cgtcgtaata
    22141 ccagttgctc tgcaacacgg atttcggcat gcgtttgagg aacacttccc gattgttcca
    22201 gatgtaatct gaccagatcc aggcgcgaac accggccttc tcaacctgct ccacgtagaa
    22261 cagaaaatcg tgccaccaga gatcgaattg gcggatgaca acgtattcga atcgccgctg
    22321 gtggttcgcg gtttcctcgt ccatgccgag atggaagaac cttggcgtat caaacagcct
    22381 gatcacctct tcgatgaggt cggcacatac cgcgtagtac gtgtcggtag aaaggcaacg
    22441 tgaataaggg cccagccata cgtcatggca ggcagaaaaa ttgagtttcg ggatcggttc
    22501 caacccgagc ccgcggatga acttcagttc ctcccggagt ttctccgggc tccatgcgcc
    22561 tcgaacggca atctccgggt ggctttcgta ccgaattccg tctccgagat cgatgattac
    22621 catattcagg ccattttcca ccatctttct gagaatgtcg cgccagagcg actcatcgaa
    22681 gcggagatac ggccgtgcgg ttgtgtagct gtcggggcgt tccggatctt cgcggtcggc
    22741 ccacatgttg tagctgaggt ggagaagatt tgctttgatc agttctgatg cagccattgc
    22801 tgcgctcctt caactgaggt ggtgggaaaa cgaaacggat caggtgtcga acgacaaggc
    22861 gaatggctca gaagcgcagg ctgtgtgccg gtgcccgacg tatgaaggct ccgttcccac
    22921 gcaggcgggc ggctgagtgt ttccagaaga gcggaaaact gcaggttggc ccgtcgaagt
    22981 gattcttcct ggaacgccac gtgtctgttc agccgggcaa attcggcgca cgttgcggtg
    23041 gtcgaatcgg gatccgcgcc aagaacgcgc gccagcgaat cgcggacagc tctggttgca
    23101 gtgagcctcc ggcgaccttc caggacgcgt tgcgcgttct cacggagggc gaatcgcagc
    23161 tcctcgaccg tcagttcccg gtacgtcttt ccgtaacgct cgaggagatc ccgttcgctg
    23221 atactccccg cgcagtagcc ttgcagcaag gaaaacagaa cgtcgccgcc ttccattgaa
    23281 gcgctttgcg gagataccgc ccaggcgttg taactccagg gataaccgga ggccggcgcg
    23341 aagctcttca gatcgtctgg aaccgggtaa tcctctagag cgctgatgaa gctgacatag
    23401 ttgcgcaggg tgtccgccgc ctccggactc gtcaaaaact gaaggaaatc aacaacctgc
    23461 gcaagatgcc cgcgccgttc agctacttta ccgatagaaa aactgagatt gtagctgcca
    23521 tcgtttctcg gtccatccgc gtacggagtt gtttctttgg tcaccaacgg cattggaaag
    23581 acgccccagt cgaaattcac agcctcggcg agtccttcgg tctcccagga tccgtccatg
    23641 tacatcgcac cgatgccgtt ggcgaacagc atcttggatt gctgacgatc cagcccattg
    23701 tatccccgca cccagtacct ggaccactct ttcagcagat gaagaacagc gagcatctgc
    23761 gggtcatcaa ggcgcgtcag gcccttgtag atcgagatat tggattcgat cccgtccacc
    23821 tgtccgtcgc gaatgaccgt gtcgatttcc ggcacgcggt cctggaacat catgtcgctg
    23881 aggaattcca tgcacgtgaa aatgtatttg tgcgggttgt tgtttgaatt tggcatcagg
    23941 tagggcacga tcccggcgtc cagcagcttc tgctgcacgg cgacaaactc tttccaactg
    24001 gttggtggtt gcgtgatgcc ggcgcgggcg aacagctcct tgttgtagaa gatccgcacg
    24061 gtgaagaaat tgaacggtac ccaccacaac tcgccgtaaa tcgggtcttc cgcgttcttc
    24121 catacaatcg ggtaaaacgt atccttccac cgttttcccg gcgcgtaagg gctctcctcg
    24181 tgaagttgtt ccgtgagggg aagcaacaac cccattcgag ccccgaagcg aaacgcgtca
    24241 cttcctccga aatccagaat gtcgggtccc ctgccgctta tgaactgagt ttccgcccag
    24301 acctggtacc cgtcaagcgg ctggtagtga agccgaacct tccacccggg gtgcagctcc
    24361 tcgtaacgcc gggccaaaac cgcgaacgcg ggaatccaac cggggtgttc tcccatgtgt
    24421 gtggcgaagt agatggtttt tgtctcgtct acggtttgtt caggcgccgt ttcgcgcggc
    24481 gcgcacccgc acatcacccc gaacagaaga cagattgctc cctgcacgac gagatttctg
    24541 caggttctga tggctggaag cacgtggtac gctaccattc gggtgccgta ctccttactt
    24601 tcttggaagg gttggtggcc tggtgccctg ttgagcaaat gagctcaatt cctccgaccg
    24661 tggaacctcc actccgctac caatggtaat tcgtatgaga ggtccacaga aaccgtcgtc
    24721 ctgtcgtgct aggagtcctg catgcctgaa gtaaccaaag aaatggttgt cgaagccctg
    24781 agcaaggtga acgaccccga gctccatcgg gatctcgtct cgctcggaat gatccgcaac
    24841 gtcgccgtgt cggatggagc ggtcaagttc acgctggcgc tcactacgat ggcctgtccg
    24901 ctgaaaagca gaatgcagga ggactgcaaa actgcagtta tgcgcatccc cggcgtcagg
    24961 gaagtcacga tagaccttac tgccgacacg ccacgcaatg cacgtccggc gtccgggaaa
    25021 aaggtcggtg atattgtgct ccaggatatg ttacccactg tgaagaacgc gattgccgtg
    25081 gcttccggga agggcggcgt gggcaaaagc acggtttcgg tgaacatcgc tgctgcattg
    25141 gcaatggaag gcgctcgcgt cggattgctg gatgcagaca tttatgggcc gagcataccg
    25201 atcatgatgg gagttacgga acaaccggag atcaccgact cagaagagat aattccgatc
    25261 aggaaatacg ggatcgagtt gatgtccatc ggcttcgtga ttcctgagga acaatcggta
    25321 atctggcgcg gcccgatggt ggcgaagatg ttgcagcagt ttctgagtgg agtgaagtgg
    25381 ggcgaacttg actatctgat tgttgacctg cctccaggca cgggtgatgc acaactgacg
    25441 ctcactcagt cggtaccttt ggccggagga ctgattgtgt cgactccgca ggcggttgcg
    25501 ctggcagacg tgcgccgcgg gatcacgatg ttcaagaaag ttgatgtgcc gattcttggc
    25561 gtggtggaga acatgagcac gtttgtgtgc cccaagtgtg gccacgagga gcacattttc
    25621 gcgtatggcg gcgcgcgaag tgaagccgaa aaactgggtg ttccgttcct gggggaaatt
    25681 ccgattgacc tctcaatacg cgaaggtggg gattccggcg ttccggtcgt ggtggcgcat
    25741 ccggattcac ccgtcgggct ggcgtttcgg aagcttgcgc gcgacgctgc ggcggcggtg
    25801 agcgtgagca acctgaccgc acggaaagcg gaacgacatg agccggcaag aaccggt
//
LOCUS       DAOTNG010000004        24891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2083_length_24891_cov_5.020213, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000004 DAOTNG010000000
VERSION     DAOTNG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24891
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_24891_cov_5.020213"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="P5179_00415"
     CDS             <1..1084
                     /locus_tag="P5179_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93715.1"
                     /translation="GEIAHVILAGTLLVFELCLPFLVGYLAAEIRDRTTGFVAFLLAL
                     FWPQLLVMSGRLTNIPIYTSLLLLACVLVLANSIPLRRRAFLSGLALGMFGLFRFEAC
                     AFLVPFGFVLFRHKNGSGSRRAGVGWRRRLGILSVFLGAFLIPLLPWIGRNYAVFGEV
                     VLSTSGGYNLRRGHHARATGTAREHYAAENTLTESTAGGTPVPGADAMARVVERTPGD
                     VLEVDRFFRDEALRFIAANPAKEASLAVRKLFFFLVADFTHPMSRVFPIWGTSFVALM
                     VGLVHWFRSGIRDPRQQFLWLVFATQACLAVAFFVVPRYRMGVEFVPLVFFAAALDPL
                     VQHWSADSTPQQKEAPVHRASPSEHP"
     gene            complement(1097..2095)
                     /locus_tag="P5179_00420"
     CDS             complement(1097..2095)
                     /locus_tag="P5179_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93716.1"
                     /translation="MAARVRVAMLGAGAIAQDHLRALRFSSRAEIVGIADVNLSLAQK
                     AAAEFGGTAFADYHDMLDRLKPSAVVVCTPPKFHRQHVEDCARAGSDVLCEKPLALTV
                     PECDFMISAVKKAGRQLMAGQVLRYYAGPKTLIEIARSGKIGTVVTCWSTRVGYYDPA
                     HGPSWRYDPKIGGGTAIEWEVHEIDLLRMLAGEAKTVSGRVLYTRPDSPEFDDHVHAV
                     MTMGEGAIGRIDASNSSYYSECTRGVVGTTGAAWCGWGDVSLRTAGSNDVQIVKPVLP
                     EVPENVNVGMFQQDDAFLEALQADRPVPIPGEEGRADVAVAAAILLAGKTGETVRL"
     gene            complement(2130..3158)
                     /locus_tag="P5179_00425"
     CDS             complement(2130..3158)
                     /locus_tag="P5179_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93717.1"
                     /translation="MYRVGIIGAGWVAGEHMRAYLNNPETEIAAIAVRRPETAQAYQK
                     SLGFSCAIYTDVAKMLHEADLDIVSICTPPSMHPEHAVAAAQAGCHLVLEKPVALNVE
                     GFRQIRAAVEAAKVKTVISFVLRWNPLFETIKALLGAEAIGRLFYGEVDYNHGIGPWY
                     GQYRWNRLKAEGGSSLLSAGLHALDALRWFVQKEAVEVFAYSTNSMNLELYPGGYEYD
                     PTMVTVLKFEDGVIGKVASNIECVQPYTFPITLQGTSGTIRNNQLYSKKLMPGQTGFA
                     TIPTILPDSGDVTHHPFQHEIDHFVQCIQLNVESHANLVDAAKTHEICYAAELSAAEG
                     HPVRLPIL"
     gene            complement(3362..3661)
                     /locus_tag="P5179_00430"
     CDS             complement(3362..3661)
                     /locus_tag="P5179_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRS93718.1"
                     /translation="MLTAIMLINAELKSVAAVTRSLAEIPEVRQLFTTTGQFDVVAVL
                     VVKDSEALAEAVTERIANVPGIVRTSTLLAIRCHAQSLLQSTFSVGFEPAEPSQS"
     gene            complement(3769..4710)
                     /locus_tag="P5179_00435"
     CDS             complement(3769..4710)
                     /locus_tag="P5179_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS93719.1"
                     /translation="MARPLGMATRQAYGKALVEIGKLHPNVVVLDADLSKSTMTKDFA
                     KAYPDRFFNVGIAEANLVGVASGLATTGKIPFASSFACFLMCKGFDQLRLGVAYAENN
                     VKIVVSHGGISIGEDGVSQMSVEDVALACELPKMVVMVPSDEHCTYQAIRAAVEHKGP
                     VFVRTGRAKAPLVYDAPPADFAIGKAITVREGKDVTVIANGMLVGPALDAAESLAEQG
                     VSVRVLDMHTVKPLDEEAVVKAAKETKGIVVAEEHLVHGGLGSAVAQVTARMHPARIE
                     FVAIQDTYAESATGEELMAKYGLSEPDVIAAIRRILA"
     gene            complement(4737..5567)
                     /locus_tag="P5179_00440"
     CDS             complement(4737..5567)
                     /locus_tag="P5179_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRS93720.1"
                     /translation="MAQYGPPQTVSQLEKTAKELRRDVIRMLYEAKSGHPGGSLSAAD
                     IVAALYFRVMRHDPQNPSWENRDRFIMSKGHAVPIVYAAMAGTGYFPKEELLTLRKLN
                     SRLQGHPSAKDLPGIEASTGSLGQGLSIGLGMALGLRMDGSDSRVFVILGDGEIEEGQ
                     VWEAAMAAAHFKTENLIAILDRNRYQLDGSTDEIMNVCPVVDKWKAFGWRVLDIDGHS
                     MHQVVHALEAAKEQTGRPTVIVAKTTKGKGVSFMENDNEYHGKAPSKDEMERALAELS
                     "
     gene            6226..8364
                     /locus_tag="P5179_00445"
     CDS             6226..8364
                     /locus_tag="P5179_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRS93721.1"
                     /translation="MTDPTGLDSELELHRWMGARIRQIRVTKNMTQDELAQRMGTQRP
                     AVSNWENGINMPSLFTLRKMAATFGVSLRQLIEPDSEDVADVPMIPLDTAVDRPGLER
                     WIRLYERLTELGDSGQRLLQTTLRIFGRLGALGDSGSGQVVYSSVLHAIQEALNFGLT
                     VLSKDDFRRTTLFLRETGLAADTEADQDRYAHLHQVFVPPAGYVDLLARMTDEQRVVV
                     ITGETHQGKTFVATKLLADIAEHGYRPIAVMGCFSGLAGDTARADTRLRDCLRPGHAV
                     LLDDPFSPGAGALALREFTTNPNLALSLVAEADCRLIITATADALKQRPDAARQLAPY
                     EWSLADKYEPTSFVRMVINYSEIYHPGAREPIVASHEGLSSPHAVERSFAVATDNKQT
                     IRRRSAGIRREGVEAITVADYDTLDNAELAFLLLLRHVPLPAHELYSIFTHLEIQGIS
                     GVVPTYDVLLDDLRRWLLVSKQDGATNIGFYHPSYASALDSILWERERVQRLAGRVLR
                     ELVTAGDPYLDRAAMSLLSVISVAGGAALGSLLREAGLADGVLLKNADPAIASLIHGV
                     MGDQGEQLAADLKGLCEVSFTKRYLSSFSLISQQARGANAASGSPQQVWNDMVNGYLS
                     DNWQVNRDLLTVLASAAVSGNARMRALAGTLAIVRGSQHDAHFSAKLIEPLQNDRQKG
                     VTRCTKRALLDYFDLVPASARDELLAATGL"
     gene            8390..9322
                     /locus_tag="P5179_00450"
     CDS             8390..9322
                     /locus_tag="P5179_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS93722.1"
                     /translation="MEYRILGRTGLRVSEIGLGALEIGRDWGIPVGTDFGRPDEKGAE
                     RLLNAALDAGITLIDTAPAYQLSEERIGKAISYRRHEFVLATKVGERLNDGDAKSHYD
                     YSYEATLAFVEQSLRRLQTDVIDVLQIHSASIEVIRAGETLRAMKKMQEQGKVRFVGM
                     TGNVDAAIEAVKTDDYDTVQVSYNVAYREAEHELFPLCLDNNIGVIIKDGLARGSLTP
                     KVSALPEKAKKQKLVAERLISECVAKSLHPNAYPKSLSDFALRFVLHQKAVTSVIAGT
                     RKVEHLLANVGASDGRRLTDEMVRKAIEIVDSVE"
     gene            9350..10123
                     /locus_tag="P5179_00455"
     CDS             9350..10123
                     /locus_tag="P5179_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS93723.1"
                     /translation="MIALSGCAEALFTTLPFNERIAAIAGLGLPAFEFWGFANKDIPG
                     IRAAMEKTGLAVSSISCDTGGPLVDPSKRSAFLPACKKSIEVAHLLGCKTLIVTVGQE
                     IPGVSRKEQHRSIVDGLKTVVSLVEDEGVTLVIEPLNVLVNHKGYFLATTSEGIDLVD
                     EVASPNVKLLYDIYHQQITEGNLIDTIKANVAKIGHFHMADVPGRHEPGTGEINYEQV
                     FRAIAETSYSGYIGLELWPTKPEKEALASTIAWAKRYFG"
     gene            complement(10187..10273)
                     /locus_tag="P5179_00460"
     tRNA            complement(10187..10273)
                     /locus_tag="P5179_00460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10237..10239),aa:Leu,seq:cag)
     gene            complement(10358..11491)
                     /gene="dgoD"
                     /locus_tag="P5179_00465"
     CDS             complement(10358..11491)
                     /gene="dgoD"
                     /locus_tag="P5179_00465"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HRS93724.1"
                     /translation="MKITAIDHFVCQAGGRNWVFVKVSTDEGVYGVGEGSLYGKEHTM
                     RSAIEELRRDIVGKDPRRVEYLWQEMYRHAFWRGGPVLCSAISGVEMALWDIKGKLLG
                     VPVYELLGGHVRDEVRFYCNLGDGTPESLIERGKKWIAKGIDAFKIYPFPAVNYVDSM
                     RPIREAEEKIASFREFAGNEVDIMIDFHGRLSPSMAILAEEVMRPYEPLFIEEPCLPE
                     NVEAMAKIARHAKTPIATGERLYTRWGLREIIERQIASVVQPDLCHCGGIMEAKKIAA
                     MAETYYMATAPHNPYGPVATAACVQVDFCIPNFLIQEHIFDDMPWRDLVYTPVIELEN
                     GRLKRPTLPGLGIDLNLEEIAKHPYEERDQVKWYHPDGAIADM"
     gene            complement(11488..12678)
                     /locus_tag="P5179_00470"
     CDS             complement(11488..12678)
                     /locus_tag="P5179_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93725.1"
                     /translation="MKVQIPFPISFWCGVPADFVAPERYKEIAEAGFTIAQVSGTRED
                     IVRQLNWCEENNITGMVLDRRVHAGMTGASDWRDTVREVVLDYSWHPALWGYYLTDEP
                     SASQFGTLAEITREFEKLDPAHPTYINLFPNYANEEQLGTATYEEHIRQYLDIVQPPL
                     VSYDHYAIQQDGNDWPIFYTNLSVVREQSLEAGKDFWQIVLSLAFHNCRNPSEADLRW
                     QVWMTLAYGGKGISYFTYWTVTPENFRDAIIDEFGYQTSHYPMVRRINRQIQNIGAAL
                     LRLKSLGVWHAPVPFKGVVPKEDYAPQFVVGSSDVPISVGEFTDGDVPALIVVNRSLE
                     RSVPAEVRVKPEFAAYREANRTSGGVSGPMKLSRSDAFASFHVWLAPGDGALLYLDRE
                     GITS"
     gene            complement(12689..13432)
                     /locus_tag="P5179_00475"
     CDS             complement(12689..13432)
                     /locus_tag="P5179_00475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS93726.1"
                     /translation="MPAEFRSPDWDSYRMDMHDPHRELEPLEREVRIVDQALERLRAA
                     GIIPHTRYDSDKMLAFRAQVAASFDIPWTAITPRQQRLIWAINAICQPKTMVAAGVFC
                     GNTFISNAGAAVGAGAAYRAERLIGLEIKQDEAERAERNVRRIDPTGVAKCVAADAVD
                     FVRDMTGEINLLYLDADGAGGRGKGVYLDILEAAWDKMPKGSIVLAHNSVNCAEKLSY
                     YLAFVRDTANMEASMNVVIDGEGLEVSAK"
     gene            complement(13422..14528)
                     /locus_tag="P5179_00480"
     CDS             complement(13422..14528)
                     /locus_tag="P5179_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93727.1"
                     /translation="MIPYEGKNPRTVNFLKTLYFDTPQWVPCGVSLMMATWMKYREEL
                     EAVVLAHPRIFPGYVKGSINLDFPDAYVLYEPGYHTDNWGCVWHNIERGLDSIVVKHA
                     LENWDDFSNWKAPDPLTQADFGGRPSLEQIKSGLAAARARGDLAGGGGLAHGFFYMRL
                     FYLRGFENLMMDFANDDPRLHRLIQIIEGYNVAVIQQYLEAGAEYMSFGEDLGTQKSL
                     PISPAMWRKFIKPSYEAMFGPCRDRDIPVYLHSDGHILEIIPDLIDVGIRMINPQIRA
                     NTLEGIRDVMKGRIAIALDLDRQLFPFASFSEIEDHIAGAYDALNMKEGGLTMGAECE
                     PDVPLERIDWICSVLERVCKPPLPEELGEKSRAS"
     gene            complement(15117..15728)
                     /locus_tag="P5179_00485"
     CDS             complement(15117..15728)
                     /locus_tag="P5179_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HRS93728.1"
                     /translation="MTQPAVLDAAAALSSFMDLKKGRLSACTAQHRRRLSDLFGVFSD
                     EEARARKAMEDDETVYESFLTEPADQPRARALCYSTTVVYPGRVGDEFYMTRGHRHAR
                     QDSPEVCLTARGRGMVLLQTDDFRIQALALEPGTVVYVPGVTSHRIVNTGDELLAVFS
                     VFPLMAGRNYRAVESHGFRRIVVATPEGPDLVPNPQFRLGKGA"
     gene            complement(15728..17347)
                     /locus_tag="P5179_00490"
     CDS             complement(15728..17347)
                     /locus_tag="P5179_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS93729.1"
                     /translation="MQQPHFSIGLTFRRARSARYGAAVRLAKGFESYCEDEDGDLLHN
                     VFLTDALKQPEVFSRFVTLVKIVADWKDTVLTMSGKVMPPAAVELLSSVGECYQRQKR
                     SGNPYHYCNGFDPRHSPTHFGCRLLSSVNRFIPHNGDIHQSDRAWYRIGELDGTVFTV
                     DKAKIIDMLEEEATHKYTDACPVFRRELVREEVQSLPDTVETAGNAKWLVEQRADGTR
                     GIRLRTIADARTLAKDGSRIVPYSQVPEGQGAWGQPATRTIPSVRFDDVGGQDKAVSD
                     IRDWVELPLRHPELFQHVGVPTQVGVILFGPPGTGKTLLAQAVAGECEAHLEMVSGPE
                     ILSRWVGQSEEAIRIIFERARSMAPSVVLLDEIDAIAPARDKTYHGHEITLVSQLLAV
                     MDGLYERGQVVVIATTNRLEAVDPALRRPGRFDYAVEMGLPDLQGREAIFQRHIRRMA
                     VGEVNIRELAERTPGMSGADIAKICRQAGLLCAKEAIAKGYDEVADVIVEARHFMAAL
                     GETGTGAPVPDAGSTTRSGRSKRRTRYHGEA"
     gene            complement(17535..18251)
                     /locus_tag="P5179_00495"
     CDS             complement(17535..18251)
                     /locus_tag="P5179_00495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS93730.1"
                     /translation="MIPFRDSNPSGTPPVVTVGIILANLAAFAYELSLGPLLEPFIGH
                     YGVIPALVTGAEGYVADGPADLVRRFLGAMFLHGGWLHLVSNMWYLWLFGDNIEDRLG
                     HLGFLGFYIGCGVAATSFHVAMDPVSPVPMIGASGAIAGVLGAYLVCFPRARIATFLP
                     VLFMPFVFEFPASLVLGSWFLIQLFSGTAAAAVPADVGGGVAWWAHVGGFVVGMVSIR
                     LLPCRSCRRQRVYAVRFDRA"
     gene            18498..19901
                     /locus_tag="P5179_00500"
     CDS             18498..19901
                     /locus_tag="P5179_00500"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HRS93731.1"
                     /translation="MSEFSREHVVASLLSTHGEVLQRGKPVVISRAPARLDVMGGIAD
                     YSGAMVLEGTLAEATICGVQLRDDQTVRIVSSANDPHGGAERDVTVPLNVLFPARTGS
                     PYARVSSEFPKSSPRHWASYGLGAYTVLMAEHVVGSFPCGATIVVHSNVPLGAGVSSS
                     AALEVAVMKALSHAYGFPMAGIPLASLCQKVENYVVGAPCGIMDQVTSVLGREGEFLC
                     LKCQPHDVQGFVRLPKGVRVVGLNSDVKHSVGGERYTSARVGAFMGQRIIQEVGLREA
                     GVDPTGGYLANISKADFRKKWARHLPQKMTGVKFLELYQRTNDPVAPVAPQTEYMVRS
                     RAAHPVYENDRVQRFISLMKTATDSGEKAPLIAAGKLMYASHWSYGQRCGLGSRETNT
                     LVSLVRQRGVSEGFFGAKITGGGSGGTVAILCRRDTDAALESIAERYHEISGRSPQLF
                     LRSGPGADQWGVIQVSF"
     gene            19960..20481
                     /gene="rsmD"
                     /locus_tag="P5179_00505"
     CDS             19960..20481
                     /gene="rsmD"
                     /locus_tag="P5179_00505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRS93732.1"
                     /translation="MKSPDTRPMTDRVKTSLFDSLNPYLLDALVLDLFAGVGGLGIEA
                     LSRGAAHCTFVERDHLCVEALQTNLRKTRLSDRATVYERDVETALTLMRSEDSPVNIV
                     LFDPPFSLGKPPARTALEALVERLADGFIERGGLLVYHHDNETEGGFKTRRFGVINTR
                     KYGRNRVTILKAL"
     gene            complement(20459..>21276)
                     /locus_tag="P5179_00510"
     CDS             complement(20459..>21276)
                     /locus_tag="P5179_00510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS93733.1"
                     /translation="AVDEFAATLGREAQKVGFSKAVQSLADGTVEYPATRAFEPAVTA
                     AEVREGRYRASESFWETFSRNRLVQQQQNAIAAHLDAMEEMWLRAEVNFADTVLDVGT
                     GWGGALVPLVERAPQDAMICGFDTAFLNLRVAQGQIERAQYDNVVFVVGDCLNPPFRE
                     QLFDAVISWFGIGRIPGFSDYLQGILRLLGPGRAFSCAWTPLVNDLDGLASQADLLGM
                     AERLDIPTRPDETAGFMEAIGFEEVELTTVGPIFVLSGRAPVREENLRGPSGS"
     assembly_gap    21277..21376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21377..21609)
                     /locus_tag="P5179_00515"
     CDS             complement(<21377..21609)
                     /locus_tag="P5179_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93734.1"
                     /translation="MKEAILEKLRCPRCREQISVWNVTEEDGGEIVEGTLACHACGGT
                     FPVHSTIPILLIPGRRAVDEFAATLGREAQKVG"
     gene            complement(21682..22182)
                     /locus_tag="P5179_00520"
     CDS             complement(21682..22182)
                     /locus_tag="P5179_00520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS93735.1"
                     /translation="MIKTTLGEIVFRLNDQDAPRHTANFRKLARQGFYNGTTFHRVIP
                     GFMIQGGDPNSKDNDRENDGTGGPGYTIPAEIKALHLRGAVSAARQADQVNPERRSSG
                     SQFFICVAPQTFLDGAYSVFGNVVRGMEVVDKIVSVPRDARDNPLTPVVIRSVTIRTL
                     RAGEQP"
     gene            complement(22616..23209)
                     /locus_tag="P5179_00525"
     CDS             complement(22616..23209)
                     /locus_tag="P5179_00525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS93736.1"
                     /translation="MRLRTSFLATLPLVVVSVILTSCGSKSREVAVISTSAGDIVVGF
                     FDKDAHRHVENFKKLAREGFYEGTTFHRVIPGFMIQGGDPNSKDADRANDGMGGPSYE
                     IPAEINMPHKRGTLAAARMGDEVNPERRSSGSQFFICVADAPFLDGKYSAFGEVLSGM
                     DVVDKIVAAPRDDRDNPITPVTINKVTLRPAKSGETR"
     gene            complement(23193..23414)
                     /locus_tag="P5179_00530"
     CDS             complement(23193..23414)
                     /locus_tag="P5179_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRS93737.1"
                     /translation="MPIFEYHCDSCGADFERLIYDRNESVACDCGNKNVKKKLSVFAA
                     QVNAGASSSKAAACDSCCMAGGGSCALKN"
     gene            complement(23470..23802)
                     /locus_tag="P5179_00535"
     CDS             complement(23470..23802)
                     /locus_tag="P5179_00535"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRS93738.1"
                     /translation="MIPAANFTRRVYDVVRRIPSGMVASYGLVALALDRPRDARQVGR
                     ALFVCREADVPCHRVVNRNGVLTGAWAFETPTEQREKLLSEGVAFDSQGRVDMALHCV
                     SVDELTDP"
     gene            complement(23799..>24891)
                     /locus_tag="P5179_00540"
     CDS             complement(23799..>24891)
                     /locus_tag="P5179_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRS93739.1"
                     /translation="NERGHVYRYAGDSAWIDCGSPDGANSVASLAVHQGCLYAGTAHY
                     HASGSSLPDSPNTFPGGKVYRYEGGQRWVDCGRLGDACEAFALSVFQESLYAIPLYSQ
                     GVFRYDGESHWEEIGRPGNCRAFALGVFNGSLFTAGNERAGVWRFDGSQWHDCGLQKD
                     VDQVYSFAVYQGALHAGTWPKAVVYRYEGGKDWADCGRLADELEVMAMAVFNGKLYAG
                     SLPLAQVYRSDGDREWTAVGRLDWTPDVRYRRVWSMAAYEGKLFAGVLPSGHVHSMEA
                     GACVTHDSELSAGWNHVAAVKEGNTLSLWVNGVKVSQRHAPRPFDITNDVPLRIGFGQ
                     QDVFNGAIRDFRLYGRALNAREIANVAAR"
BASE COUNT         5123 a         7514 c         6965 g         5189 t
ORIGIN      
        1 tggcgagatt gcacacgtga tcctcgccgg cactcttctt gtcttcgaat tgtgtctgcc
       61 atttcttgtc gggtatcttg ccgccgaaat ccgggacaga actaccggtt ttgttgcgtt
      121 tctcctcgct cttttctggc cccagctcct cgtcatgagc gggcgcctga caaacatccc
      181 gatttacact tcgctattgc ttctggcgtg cgtcctcgtc ctggccaatt cgatcccgtt
      241 acgcagacgc gcattcctga gcgggctcgc gctcggcatg ttcgggctct tccgcttcga
      301 agcgtgtgcg tttctcgttc cgttcgggtt tgttctgttt cgccacaaga acgggtccgg
      361 ctcccggcgc gcgggcgttg gctggcgccg ccggctggga atcctttccg tgttcctggg
      421 agccttcctc atcccgttac tcccgtggat tggacgcaac tacgcggttt ttggtgaagt
      481 tgtgctgagc acgtccggcg gatacaacct tcggcgaggg caccacgcac gcgccacggg
      541 aaccgcgcgc gaacattacg ccgcagaaaa cactctgacc gaaagcacgg caggcggtac
      601 gccagttcca ggcgccgacg cgatggctcg cgtggtggaa cgcactccgg gagatgttct
      661 cgaagttgac cgcttctttc gcgatgaagc actgcggttc atcgcagcaa atcccgcgaa
      721 ggaggcgagc ctggcggtga gaaagctatt cttcttcctc gtggcggact tcacccatcc
      781 gatgtcccga gtgttcccga tttggggtac atcgtttgtg gctcttatgg tcgggctggt
      841 tcactggttc aggagcggaa ttcgcgaccc ccggcagcag ttcctctggc tcgtgttcgc
      901 cacacaggca tgcctcgcag tggcgttctt cgtcgtgccg cggtaccgca tgggcgttga
      961 gttcgtgccc ctcgtgtttt ttgctgctgc ccttgaccct cttgtccagc actggtcggc
     1021 cgattccacc ccccaacaga aagaggcgcc ggtgcaccgc gcctccccct ccgaacaccc
     1081 gtgacgagag taaggcctaa agccgcaccg tttccccggt tttgccggca agcagaatcg
     1141 ccgctgccac tgcaacatcg gccctgccct cttcaccggg gatgggaacg ggtctgtcag
     1201 cctgaagcgc ttcgaggaat gcatcatcct gctggaacat gccgacgttg acgttctccg
     1261 ggacctctgg aagcaccggc ttcacaatct gcacgtcgtt gcttcccgcg gtgcgcagcg
     1321 atacatctcc ccagccgcac cacgccgcgc ccgtggttcc taccacgcca cgggtgcatt
     1381 cggaatagta tgacgagtta cttgcgtcta tgcggccgat ggcgccttcc cccatcgtca
     1441 tgacggcgtg cacgtggtca tcgaactcgg gagaatccgg gcgcgtgtac agaacccgcc
     1501 ccgaaaccgt tttcgcctca ccggccagca tgcgcagcag atcaatctcg tgaacttccc
     1561 attcgatcgc cgtaccgccg ccgattttgg ggtcatatct ccaggagggg ccgtgagccg
     1621 ggtcataata gcccacgcgc gtactccagc aggtaacaac cgttccgatt ttccctgagc
     1681 gcgcgatttc tatcagcgtc ttcgggccag cgtaataccg aaggacctgt cccgccatca
     1741 actgccggcc cgcttttttc accgccgaga tcatgaaatc gcattccgga acggtgagcg
     1801 cgaggggttt ttcgcagaga acatccgagc cggcccgtgc gcagtcctcc acgtgctgtc
     1861 ggtggaattt cggtggcgta cacactacca ccgcagaagg cttgaggcgg tccagcatat
     1921 catggtagtc ggcaaatgcg gtgcccccga actccgccgc cgccttctgc gccagactga
     1981 gattcacatc tgcaattccc acaatttcag cgcgcgaaga aaaccgcaac gcccgaagat
     2041 ggtcttgcgc gatagcgccg gcacctaaca tggcaacacg aacccttgcc gccataaagt
     2101 cccctttccg aaagaaaagc ccctgcgggt tacagaatcg gcaaccgaac cgggtggcct
     2161 tctgcagctg aaagttctgc cgcgtaacat atctcgtgcg tttttgcggc atccaccagg
     2221 ttcgcgtggg attctacatt gagctggata cactggacga aatgatcgat ttcgtgctgg
     2281 aacgggtgat gtgtgacgtc gcctgagtcc ggcaggatag tgggaattgt ggcaaaaccc
     2341 gtctggcccg gcatgagctt cttcgagtag agctgattgt tccggatcgt tcctgacgtg
     2401 ccctggagcg tgatggggaa ggtgtacggc tgtacgcact cgatgttcga cgccactttc
     2461 ccgatcacgc cgtcctcgaa cttgagtacc gtcaccatgg tcggatcgta ctcatagcca
     2521 cccgggtaca gctcaagatt catgctgttc gttgaatacg caaagacttc cactgcctct
     2581 ttctggacga accaccggag tgcgtccaat gcgtggagcc ccgcggacag cagcgaactg
     2641 ccgccttctg ctttgagccg gttccacctg tactggccat accaaggccc gatgccgtga
     2701 ttgtaatcca cctcgccgta aaacaatcgc cctatcgctt cggcgccgag gagcgccttg
     2761 atcgtctcaa aaagaggatt ccacctgagg acgaaactga taacggtctt cacctttgcc
     2821 gcctcgactg cggcacggat ttgtctgaat ccttcaacat tcaatgcaac cggtttctcc
     2881 agaacgagat ggcagcccgc ttgtgcggcc gccaccgcat gttccgggtg cattgacggt
     2941 ggggtgcaga tggagacgat gtccaagtct gcttcatgaa gcattttcgc gacgtccgtg
     3001 tagatcgcgc aggagaagcc aaggcttttc tggtacgcct gcgctgtctc cggccgccgg
     3061 acggcgatcg cggcaatttc cgtttcaggg ttattcaggt acgcccgcat gtgttctccg
     3121 gcgacccacc cggcaccgat gattccgacc cgatacatgg ctctcccctt tccgcagaac
     3181 ggccggctgg ccaaagttcc attccgcgca tcgtttcaga ttcctgccag aaacgacgcc
     3241 gcacatcgaa agaccgaagg cgcgcacaag tgcccgttcg ttcacgagaa agtatactca
     3301 gcgtgcggga gatgttccaa cggaaccggc gaagggttgc cgcgcacccg gaatccggcg
     3361 atcagctttg agagggttct gcgggttcaa agcctacgga gaaggtgctc tgaagaagag
     3421 actgcgcgtg gcatcgaatc gccagcagag tgctggtgcg gactatccca ggaacatttg
     3481 cgatccgctc ggtgacggcc tctgcgaggg cctctgagtc tttcaccaca agaacggcga
     3541 ccacatcgaa ctggcccgtg gtagtgaaca attggcgcac ctcaggaatc tcggcaagcg
     3601 agcgggtaac ggcagccacg cttttgagtt ccgcattaat caacattatc gccgtgagca
     3661 tggagttcct cgcgcgtgca aaacaggcgg acagcctgat cagtggttcc ggcaagacag
     3721 aaaaaggggg cgggaccgcc ccccggggtc atttcttctt acgtgttctc acgccaggat
     3781 ccggcggatg gcagcaatca cgtcgggctc ggaaagacca tacttggcca tcagttcctc
     3841 gcctgtcgcc gattccgcat acgtatcctg aatcgcgacg aattcgattc gggcgggatg
     3901 catacgagcg gtcacctgcg cgacggcgct tcccagtcct ccatggacca ggtgttcctc
     3961 cgcaacaacg attcctttgg tttcttttgc ggctttcacc accgcttcct catccaacgg
     4021 cttgacggtg tgcatgtcga gaacgcgcac gctcacgccc tgctccgcca ggctttctgc
     4081 cgcatccagt gccggaccga caagcatgcc gttggcgatg acagtcacgt ccttgccttc
     4141 ccgcacggtg atggccttgc cgatggcgaa atccgcgggc ggtgcatcgt acaccagggg
     4201 agcctttgcc cttcccgttc gcacgaacac ggggcccttg tgctcgactg ccgcgcgtat
     4261 tgcctgatac gtgcaatgct cgtcagacgg caccatgacg accatcttcg ggagctcaca
     4321 cgccagcgcc acgtcttcca cgctcatctg ggatacaccg tcctcgccga tcgaaatccc
     4381 tccgtggctg accacgatct tcacgttgtt ttccgcgtac gcgacaccaa gccgaagctg
     4441 gtcaaacccc ttgcacatga gaaaacacgc aaagctggaa gcaaacggta ttttccccgt
     4501 cgtcgccaga ccggaggcaa cgccgacgag gtttgcttcc gcgataccca cgttgaaaaa
     4561 cctgtcaggg tatgcctttg caaagtcttt tgtcatggtg gatttcgaga gatcagcgtc
     4621 cagcacaaca acgttgggat ggagcttacc aatttcaacc agagctttcc cgtacgcttg
     4681 ccgcgttgcc attcccaatg ggcgcgccat gttgtatctc ctctttccgg attgatttac
     4741 gacagttccg ccagggcgcg ctccatttcg tctttgctgg gcgccttacc gtggtactcg
     4801 ttgtcattct ccataaacga cacccctttg cccttggtcg tttttgcgac gatgacagtg
     4861 gggcgacccg tttgttcctt tgcggcttcc agagcatgaa cgacctgatg catggaatgc
     4921 ccgtcgatgt cgagcacacg ccacccgaac gccttccact tatccactac ggggcacacg
     4981 ttcatgatct cgtccgtcga accatcaagc tggtaacgat tccgatcgag aattgcgatc
     5041 aggttttccg ttttgaaatg cgccgccgcc atcgcagctt cccagacctg gccttcttcg
     5101 atttcgccgt caccgagaat gacgaatacg cggctgtcgc taccatccat gcgaaggccg
     5161 agcgccattc caaggcctat cgagaggccc tgcccgagtg atccggtcga ggcttcgata
     5221 cccggcaaat ctttcgccga aggatgcccc tgcagccggc tgttcaactt gcggagcgtc
     5281 aacagctcct ctttcgggaa gtacccggtt cccgccatcg cggcatacac gatgggcacc
     5341 gcgtgcccct tgctcatgat gaagcggtcg cggttctccc aagacgggtt ctgcggatca
     5401 tgtcgcatca ctcggaaata gagagccgcc acgatatccg ccgccgaaag cgaacctccc
     5461 ggatgccctg atttcgcctc atacagcatg cgaatgacgt ctcgccgcaa ttccttcgcg
     5521 gttttttcaa gctgggaaac ggtctgcgga gggccgtatt gagccatgaa aacctcccga
     5581 aaaagacaga aacattcctc gcggtccgtg tactcggggc gcggaaacga atgactcacc
     5641 gactctctca aaatacaaac cgctccgtac cggagccaac gacgcgtacc agtgattcgt
     5701 gactcgggcg gtgtgtgccg ggcgtctgcc gggaagaccg gatcgcgttg ctaaagcggt
     5761 actactccac gggacggttg cgcgcccgtg cgcgaacgtg ctgggtcatg acataccgtg
     5821 cgaacaccgc cgtgagtctc cttttctgtc gagcgcatcc cgcgcgtacg aaactttatg
     5881 cgccggtggg agaggttctc ctccccgctc acgacctccg ttctcactca cccgcggtca
     5941 ccgaatccgg cgcagcaact cagctccgtc cgggtggttt cctgcgcgca atctgcacca
     6001 tgatcattgt tcacgacggc gccaagcatc aggttcaagt tcttgttttt aagaacttat
     6061 aagatcgcca ttcggcccgc cttgcgtatg actctttcga aagatttggt gaaggagcag
     6121 tttgtcgcgt atgatgtgtc ttgtatattt ggtgcagtcg gcattgtggg aaatgccccc
     6181 tgacgatcac cccagaccgt atccgcttca agagggagtt tgtgaatgac tgacccgacc
     6241 ggcctcgata gcgaactgga actccatcgt tggatggggg cgagaatccg tcagatacgt
     6301 gttaccaaaa acatgacaca ggacgaactc gcccagcgga tgggaacgca acggcctgcg
     6361 gtttcgaact gggaaaacgg gatcaacatg cccagtttgt ttacgctccg caagatggcc
     6421 gcgaccttcg gcgtctcctt gcgacaactg atcgaacctg attcggagga cgtggcagac
     6481 gtaccgatga ttcccctcga caccgccgtg gaccggccgg gcctcgaacg gtggatacgg
     6541 ctgtacgagc ggctcacgga actcggggac tccgggcagc gccttcttca gacgacgttg
     6601 cgcatttttg gtcggctcgg agctctcggt gacagcggaa gcgggcaggt agtgtattcg
     6661 tcggttcttc acgcgatcca ggaggcgctc aatttcgggc tcacggttct ctccaaggac
     6721 gatttccggc gaacgactct gttcctcagg gagaccggcc tcgcggcgga taccgaggca
     6781 gaccaggatc ggtatgccca tctccaccag gtgttcgtgc ctccagccgg gtatgtggac
     6841 cttcttgcgc ggatgacgga cgaacaacga gtggtcgtga tcaccggtga gacccaccag
     6901 ggcaaaacgt tcgttgccac caagcttctg gccgacattg cggaacacgg gtaccgcccg
     6961 atcgcggtca tgggctgttt ttcgggactt gccggcgaca cggcacgggc tgacacgcgc
     7021 ctccgggatt gcctgcgtcc gggacacgcg gtgctgctgg acgatccatt ttccccgggt
     7081 gcgggtgccc tcgccttgcg cgagttcacg acaaacccca atctggcgct gagcctcgtt
     7141 gccgaagccg attgccgtct catcatcacc gcgaccgcgg acgcactgaa acaacgtccg
     7201 gacgcggcga gacaactggc accgtatgaa tggtcgctcg cggacaaata cgaacccacg
     7261 tccttcgtac gaatggtgat caactacagt gagatctacc acccgggcgc gcgggaaccg
     7321 attgttgcct cacacgaggg gttatcaagc ccgcatgcgg tagagcggtc attcgcagtt
     7381 gcaacggaca acaagcagac catacgccgt agatcagcgg ggattcgccg cgaaggggta
     7441 gaggcgatca ctgtggcaga ctacgatacg ctggacaatg ccgagcttgc attcctgctg
     7501 cttttgcggc atgttccctt gcccgctcac gagctctatt cgattttcac gcacctcgag
     7561 atccagggaa taagcggggt agtaccgacg tacgacgtgc tgttggatga cctgcggcgc
     7621 tggcttctgg tttccaaaca ggacggagca accaacatcg gcttttatca tccgtcgtac
     7681 gcgtccgcac tggacagcat tctctgggag cgcgaacggg ttcagcggct ggcgggacgc
     7741 gtgttgaggg aactcgtcac ggcaggagat ccgtacctcg atcgcgccgc gatgtcgctt
     7801 ctttccgtga tttctgtcgc aggaggggct gcgctcggca gcctgttgcg cgaggcagga
     7861 cttgctgatg gcgttcttct gaagaacgca gaccctgcca tcgccagcct catccacggc
     7921 gtgatgggag accagggcga acaacttgcg gcggatctga aaggcctgtg cgaggtttcc
     7981 ttcaccaagc ggtatttgtc gtcattctcg ctgatttccc aacaggcacg cggtgcaaac
     8041 gccgcttctg gatcacccca acaggtgtgg aacgacatgg tgaatgggta cctgtccgac
     8101 aactggcagg tgaatcgtga tctcctgaca gttctcgcca gcgccgcggt cagcggaaac
     8161 gctcgaatgc gggcactcgc gggaacactg gcgatcgttc gcggatctca acacgacgct
     8221 catttctccg cgaaactgat cgagccgttg cagaacgacc gccaaaaagg ggtaacaagg
     8281 tgcacaaaac gcgcgctgct ggattacttt gacctcgtgc ccgcgagcgc tcgcgacgag
     8341 ctgctggcgg caaccgggct gtaaaccttt tgtggattgg agtgttccgg tggaatacag
     8401 aattcttgga cgaacaggcc tgagagtaag cgaaatcggt ctgggcgctc tggaaatcgg
     8461 cagagactgg ggaattccgg taggtaccga tttcggacgc ccggacgaaa aaggcgccga
     8521 acgcctcttg aacgcggcgc tggatgccgg catcacgcta atcgacacgg cacctgcgta
     8581 ccaacttagc gaggaaagaa taggaaaggc gatcagttac cgccgccacg agttcgtgtt
     8641 ggcgacgaag gtcggcgaaa ggctcaacga cggggatgcg aaatcgcatt acgattattc
     8701 ctatgaggcg acactggcgt tcgtggagca aagccttcgg cgccttcaga cggacgtcat
     8761 cgacgttctt cagatccaca gcgcgtcaat tgaggtcatc cgggcgggag aaaccctgcg
     8821 cgccatgaag aaaatgcagg aacagggcaa ggtccggttt gtaggtatga cagggaatgt
     8881 cgacgccgcg atagaggcag tgaaaactga tgattacgac acggtgcagg tttcgtacaa
     8941 tgtcgcatac cgcgaggcgg aacacgagct gtttcctttg tgtctggaca acaacatcgg
     9001 cgtgatcatc aaggatgggc ttgcgagagg ttcgttaacc ccaaaagtgt cggcgctccc
     9061 ggagaaggca aaaaagcaga agttggtcgc cgaacgcctg atttcggagt gtgtcgcgaa
     9121 atccctccac ccgaatgcct acccgaagtc gctgtctgat ttcgcattgc gtttcgtgtt
     9181 gcatcagaaa gcggtcacct cggtgattgc gggaacgcgg aaagtcgagc atcttctggc
     9241 gaacgtggga gcctctgacg ggcggaggct gaccgatgag atggtccgaa aagcaataga
     9301 gatagttgat tcggtggaat gagttgaccc tcgacgaaag gcactggaaa tgatcgcgtt
     9361 gtctggatgc gcggaagcat tgttcacgac acttcccttc aacgaacgaa ttgccgccat
     9421 cgcggggttg gggcttccgg cattcgagtt ctggggtttt gcgaacaagg acatccccgg
     9481 gattcgtgcg gcgatggaga aaacagggct ggctgtttcc tcgatctcct gcgatacggg
     9541 aggaccgctc gtcgacccat cgaaacggtc ggcgttcctg cctgcgtgca aaaagtcaat
     9601 cgaggttgcg cacctgctgg gctgcaagac gcttattgtc accgtggggc aagagatacc
     9661 cggagtcagc cggaaggaac agcaccggtc aattgtggat ggattgaaga ccgtggtttc
     9721 gctggtcgag gacgaagggg ttacactcgt catcgaaccg ctcaatgtcc tcgtgaatca
     9781 caagggttat ttcctggcaa ccacctcaga aggaattgac ctggtggatg aagtcgcaag
     9841 cccgaatgtg aagcttctct atgatatcta tcatcaacaa atcaccgaag gcaatctgat
     9901 cgacacgatc aaggcgaatg tcgcaaaaat tggccatttc catatggcgg acgttccagg
     9961 gcgccatgag ccgggaacgg gggagatcaa ctacgagcag gtcttccgtg cgattgccga
    10021 gacttcgtac tccgggtaca ttggtctcga gctgtggccg accaaaccag aaaaggaagc
    10081 gctcgcatca acgattgcgt gggcgaagcg ctatttcggt tagggagaga aggctctaca
    10141 aaggaatgag gctcctgccg cgtggcagca ggagcctcat ttaatttggt gcggaagggg
    10201 agacttgaac tcccacgagc ttgcgctcac tggaccctga atccagcgcg tctaccaatt
    10261 ccaccacttc cgcacaagta gaaggatttt gaaatttacc cgaatcgccg ccttccgtca
    10321 accgttccga cgtcttgtca cgtaacgaga cacggcgtta catgtccgcg attgcgccgt
    10381 ccgggtggta ccacttcacc tggtcccgtt cctcgtacgg gtgcttcgca atttcctcga
    10441 gattaaggtc aatcccgagc cccggaagtg tcggacgctt cagcctgccg ttttcgagtt
    10501 cgatcacggg cgtgtagaca aggtcacgcc atggcatgtc gtcgaaaata tgctcctgaa
    10561 tgaggaagtt gggaatgcag aaatccacct gaacacacgc ggccgtcgca acgggaccgt
    10621 acgggttgtg cggcgcggta gccatgtaat acgtctccgc catcgccgcg atcttcttcg
    10681 cctccatgat tccgccgcaa tggcagaggt caggctgaac aaccgatgca atctgccgct
    10741 cgatgatctc gcgaagtccc caccgggtat acaggcgttc accggtcgca atgggtgttt
    10801 tcgcgtggcg ggcgatcttt gccatcgctt cgacattttc cggaaggcag ggctcctcga
    10861 taaacaaggg ctcgtacgga cgcatgactt cctctgcgag gatcgccatg gaagggctca
    10921 accgaccgtg gaaatcaatc atgatatcca cctcgttccc ggcgaattcg cgaaaactgg
    10981 cgatcttttc ctccgcttca cggatcggtc tcatcgaatc aacgtaattc accgcgggaa
    11041 aagggtagat tttgaacgcg tcaatccctt tcgcaatcca ctttttgcct cgctcgatca
    11101 ggctctccgg agtcccgtcg ccaagattgc agtaaaaacg gacctcatcc cgcacatggc
    11161 cacccagaag ctcgtacacc ggaactccaa gcaacttccc cttgatatcc cagagtgcca
    11221 tttcgactcc acttatggcg ctacagagca ccgggccacc tcgccagaat gcatgacgat
    11281 acatctcctg ccaaaggtat tccacgcgcc gtgggtcctt gccgacgatg tccctccgga
    11341 gttcctcgat tgcggaacgc atggtgtgct ctttgccgta cagcgagcct tcacccaccc
    11401 cgtacacacc ttcatccgtg gagaccttca cgaaaaccca gttccgaccg cctgcctggc
    11461 atacgaagtg gtcaattgcc gtaatcttca cgatgtaatc ccttctcgat cgaggtagag
    11521 caacgcgccg tctccggggg cgagccagac gtggaatgaa gcgaaggcgt cacttcgcga
    11581 cagcttcatg gggccggata caccgccgct ggtgcggttc gcttcgcggt acgcggcgaa
    11641 ttccggtttg acccttacct ccgctggcac actccgttcc agggagcggt tcaccactat
    11701 gagcgccgga acgtctccgt ccgtgaactc gcccacagaa atcggcacat cgctggaacc
    11761 gaccacgaat tgcggcgcat agtcttcttt cggtacaacg cccttgaaag gcactggcgc
    11821 atgccacacc ccaagggact tcaggcgcaa gagggctgca ccaatgttct gaatctggcg
    11881 gttgatccgc cgaaccatcg ggtaatgcga cgtctggtat ccgaactcgt cgatgatcgc
    11941 gtcccggaaa ttctcaggag taacggtcca gtaggtgaaa taactgatcc ctttgccccc
    12001 gtaggcgagc gtcatccaca cctgccaccg gaggtctgct tccgaaggat tccggcagtt
    12061 gtgaaacgcg agcgataaca caatttgcca gaagtctttt cccgcttcaa gtgactgctc
    12121 acgaacaacc gagagattgg tgtaaaagat gggccagtca ttgccatctt gctggatggc
    12181 gtaatggtcg tagctcacca gtggcggctg gacgatgtcc aggtactgcc ggatatgttc
    12241 ctcgtacgtc gccgtgccca gttgctcttc gttcgcgtag ttcggaaaaa ggttgatgta
    12301 cgtggggtgc gcgggatcca gtttttcgaa ttcccgcgtg atttcggcga gtgttccaaa
    12361 ctgggaagca gagggttcat ctgtcaggta atacccccag agtgccgggt gccaggagta
    12421 gtcgaggact acctccctca ccgtgtcacg ccagtcggaa gcgcccgtca tgcccgcgtg
    12481 gacacggcga tccagcacca ttcccgtgat gttgttctct tcacaccaat tgagctgccg
    12541 aacgatatct tcgcgtgttc cggaaacctg ggcgatcgtg aagccggctt cggcgatttc
    12601 tttgtatctc tccggagcca cgaagtcagc gggcactccg caccagaagc tgataggaaa
    12661 tggaatctga actttcatcg cgttcccctc acttggcaga tacctcaagc ccttcgccgt
    12721 ctatcacaac attcatgctt gcctccatat tggcggtatc acgcacgaac gccagatagt
    12781 acgaaagttt ttccgcacag ttgacgctgt tgtgcgcgag aacgatggat ccctttggca
    12841 ttttgtccca cgcagcctcg aggatgtcga gatacactcc cttgccacgg ccgccggcac
    12901 cgtctgcatc caggtacaaa aggttgattt ccccggtcat gtctcgcacg aaatcaacgg
    12961 cgtcagcagc cacacatttc gccacgccgg taggatctat gcggcgcaca tttcgttctg
    13021 cgcgctccgc ctcgtcctgt tttatctcca acccgatgag tcgttcggca cggtaggcag
    13081 cgcccgctcc cacggcggcg ccggcgttgg aaatgaacgt gtttccacaa aaaacacccg
    13141 ccgccaccat cgtcttcggt tggcagatgg cattgattgc ccatataagc cgttgttggc
    13201 gaggagtgat ggctgtccac gggatatcaa agctggctgc cacctgcgcc cggaatgcaa
    13261 gcatcttgtc agaatcgtac cgggtgtgtg ggataattcc cgctgcgcga agacgctcca
    13321 gagcctggtc cacgatccgg acttcgcgtt ccaacggctc cagctcgcgg tgcggatcat
    13381 gcatgtccat gcggtaagag tcccaatcgg gactgcgaaa ttcagctggc acgggatttc
    13441 tctcccagtt cctcaggcaa tggcggcttg cagacacgtt ccagaacgga acagatccag
    13501 tcaattcgtt cgagcggcac atccggttcg cattccgcac ccattgtgag gccgccttct
    13561 ttcatgttga gtgcgtcgta tgcgccggca atatgatcct caatctccga aaaggacgcg
    13621 aaagggaaca gctgcctgtc gaggtccagc gctattgcga tgcgaccttt catgacgtct
    13681 ctaatccctt ccagggtgtt ggcccttatc tggggattga tcatccggat tccaacgtca
    13741 atgaggtcgg gaatgatctc gaggatatgg ccgtcggaat ggagatacac cggaatgtcg
    13801 cggtctcggc acgggccgaa catggcttcg tacgagggct tgatgaattt ccgccacatc
    13861 gccgggctga tcgggagcga tttttgagtg ccaagatcct cgccgaacga catgtactcg
    13921 gcaccggctt ccagatactg ctgaattacg gccacattgt agccctcaat gatctggatc
    13981 agcctgtgaa ggcgcgggtc gtcgttggca aaatccatca tgaggttttc gaacccgcgg
    14041 agatagaaaa ggcgcatgta aaagaacccg tgcgccaatc ctccaccccc cgcgagatca
    14101 ccgcgagcgc gggcggcagc caagccactt ttgatctgtt ccagagaagg cctcccgcca
    14161 aaatcggcct gcgtcagcgg atccggagcc ttccagttgg aaaagtcatc ccagttttca
    14221 agggcatgtt tcaccacaat gctgtcgagt ccccgctcga tgttatgcca gacacacccc
    14281 cagttatccg tgtgataccc cggttcgtac aacacgtacg cgtcggggaa gtcgaggttg
    14341 atactccctt tcacgtaacc cgggaatatc ctcgggtgcg cgagaacaac ggcctcgagt
    14401 tcttccctgt atttcatcca cgtcgccatc atcaggctca ccccgcacgg aacccactgc
    14461 ggcgtatcga agtacagggt tttcaaaaag ttgactgtgc gggggttttt gccctcataa
    14521 ggaatcatcc aaagtcccct gttgctgttg acctacaaaa gcgagagtcc gtcaacgctg
    14581 acgtgccacc gcatcccacg tgcgctccag atgctggcgg atcggaaggt agtgatgatt
    14641 cgcgggcctc atgtaacccg gctgcgggca atctgcggtt tcgtcttcgc cgactgaatt
    14701 cacgcccctg tcgtcgccaa atctcccgag gcgatcggga tcttcgaccg gcatcacgcc
    14761 agttaagtaa ccgttgccgc gtgaaccggg tcaagggagt agtgcattgc acggggttcc
    14821 ggctccgccc ggcaccggtc tcaagacgcg catggtgccg ggtggaacta acgtgagtgg
    14881 aaagggggta aaagggtgca aatacgccgg ttgatgttct cttcgaaaaa gggctgtgca
    14941 gtcataccgg cgaactgcgc agccgggaga ggccgtgatc gggagttccg gtccggtctc
    15001 tcttttttcc caggggacct tctcgagcat cccgctgctg tcagcagcat cgaagcttgg
    15061 aagataacgc ctgagagggt ccccgcagcc tcacgaacac gcggctgtcg cgcgttctat
    15121 gcgcctttcc ccaggcgaaa ctgcggattc ggcacaagat caggaccctc cggggttgcc
    15181 acaacgatcc gcctgaaccc gtgcgactcc accgccctgt aatttcgccc ggccatgagg
    15241 ggaaacacgg agaaaactgc caggagttca tcgcccgtgt tcacgatccg gtgcgaggtt
    15301 actcccggaa catacaccac agtacccggt tcgagcgcaa gggcctgaat ccggaaatcg
    15361 tcagtctgga ggaggaccat accccgccct cttgccgtca ggcacacctc gggtgaatct
    15421 tgccgcgcgt gtctgtggcc gcgggtcata tagaactcgt cgccgacccg gcccgggtac
    15481 acgactgtgg tggagtaaca aagtgccctc gcgcggggtt gatcagccgg ctcggtcaga
    15541 aacgactcgt acaccgtttc gtcgtcttcc attgctttgc gggcgcgcgc ttcttcatcc
    15601 gagaacaccc cgaacaaatc cgacaatctc cggcggtgtt gcgcggtaca tgcgctcaga
    15661 cggccttttt tcaggtccat gaatgacgaa agggcagcag cggcgtccag aacagcaggt
    15721 tgcgtcatca agcctctccg tggtaacgcg ttctacgctt tgagcgcccg gaccgagttg
    15781 tggacccagc atccggaacg ggggctcctg ttcccgtttc ccccaatgcc gccatgaaat
    15841 gcctcgcttc aacaatcacg tccgcaacct catcataccc tttcgcaatc gcttccttcg
    15901 cgcacagaag tcctgcctga cgacatattt tcgcgatatc cgcgccggac atccccggcg
    15961 tacgttctgc cagttcgcgg atattcacct ccccgaccgc catcctccgg atgtgtcgct
    16021 ggaagatcgc ctcgcggccc tgaagatcgg gaaggcccat ttcaacggcg taatcaaacc
    16081 gccccggccg gcgaagtgcg ggatccactg cttccagacg gttcgtcgtg gcaatcacta
    16141 cgacctgtcc tctctcgtac agcccgtcca tcacggccaa caactgggat acgagggtta
    16201 tctcgtgccc gtgataggtc ttgtcccgtg ccggcgcgat ggcgtcaatc tcgtccaaca
    16261 acactactga cggcgccatg ctcctcgcgc gctcgaaaat aatccgaatc gcttcttcgg
    16321 actgccctac ccatcgggaa aggatttcag gcccgctcac catttcgagg tgagcctcgc
    16381 attctccggc gacagcctgg gccagcaacg tctttccggt accgggaggc ccgaagagaa
    16441 tcactcccac ctgggttggc acgcccacat gctgaaaaag ctcggggtga cggaggggca
    16501 gctctaccca gtcccgtatg tcggaaacgg ctttgtcctg tccgccgacg tcgtcaaagc
    16561 gcaccgaagg gatcgttcgg gttgccggct gtccccaggc cccctggcct tctggtacct
    16621 gcgaataggg aacgatacgc gagccatcct ttgcaagtgt gcgtgcatct gcaatcgtcc
    16681 gaagccttat tccccgggtt ccgtcggctc gctgctcaac aagccatttg gcattgccgg
    16741 cagtttcaac cgtatccggc aacgactgaa cttcttccct caccagttcc cggcgaaaga
    16801 ccggacacgc gtccgtgtat ttgtgggtgg cttcttcttc cagcatgtcg atgattttcg
    16861 ccttgtccac ggtaaatacg gtcccgtcca gttcaccgat gcggtaccac gcgcggtccg
    16921 actggtggat gtcgccgttg tgaggaatga atcggttcac ggaagagagc agccggcacc
    16981 cgaaatgtgt cggcgaatgg cgcggatcga acccgttgca atagtggtag ggattccccg
    17041 aacgcttctg ccgttgataa cattcgccga cagagctgag caactcaacc gctgcgggcg
    17101 gcatcacctt tcctgacatg gtgagcacgg tgtctttcca gtccgcaacg attttcacca
    17161 gggttacgaa ccgactgaac acttccggtt gcttcaacgc atcagtgaga aacacattgt
    17221 gcaacaggtc gccatcctca tcctcgcagt aactctcgaa gcccttcgca agccgtactg
    17281 ccgcgccgta ccgagccgac ctggcgcggc ggaacgtgag ccctatggag aaatgaggct
    17341 gttgcatgat tgttggctac atctgatgcg tgcgattcgg tccgggcttc caacacagaa
    17401 cgagaagatt cgccgtcaaa caccacggcg aatcaaaaaa tacaaccaaa tcgcgtccaa
    17461 acgccagtcc gcggcggagg gaagagggag atcgtcgatt gtgcaggggg aatccgtgag
    17521 cggaacacgc ggattcaggc gcgatcgaac cgcaccgcgt agacgcgctg gcgtcggcag
    17581 gatcggcacg gaagaagtcg aatggacacc attccgacca cgaagccgcc cacgtgcgcc
    17641 caccaggcaa ctccgcctcc gacgtctgca ggcactgcgg ccgccgccgt tccgctgaaa
    17701 agctggatga gaaaccagga gcccaacacc agcgaagcgg ggaattcgaa cacgaacggc
    17761 atgaacagca ctggaagaaa cgttgcaatc cgcgcacggg gaaagcatac gaggtacgcc
    17821 ccaagaacac cggcaatcgc gccgctggcg cctatcatcg ggacgggtga aaccggatcc
    17881 atggcgacat ggaacgaagt agccgcaacg ccgcacccga tgtagaaacc cagaaacccg
    17941 agatgtccca gtcgatcctc gatattgtcc ccgaaaagcc acaggtacca catgttgctc
    18001 accagatgca accagcctcc gtgcagaaac atcgctccca gaaatcgcct cacgaggtcc
    18061 gcgggtccat ccgctacgta tccttcagcg ccggtcacaa gagccggtat tacgccgtag
    18121 tgaccgataa atggctcaag cagcggaccc aggctgagtt cgtacgcaaa ggccgccaga
    18181 ttggcaagga taatgcctac cgtaaccaca gggggcgttc ccgaggggtt gctgtcacgg
    18241 aacggtatca tgtcgcgctg ctcgattctg tccgtctgaa gatgggtgga accacaggga
    18301 aagatgttcc cgcgtaagac cctgcagggc cccgaatgcg tccaattcaa tataggcacg
    18361 cgtcagttca ggcagaacct caacaggtac cgcacgtgca tgatcaggcg ccgccgggaa
    18421 cggttgagcg gggcaagcgg cccccgtatc tttttctcgg cattgttccg tcaacgagga
    18481 gaaggggatc gctgatcatg tctgaattca gcagggaaca cgtcgtcgca tcgctgcttt
    18541 ccacgcacgg cgaggtgctt caacgcggaa agcctgttgt gataagcagg gcaccggccc
    18601 gcctggacgt catgggcggt atcgcggatt attccggtgc gatggtgctt gaagggacac
    18661 tggcagaagc gacgatttgc ggggtccagt tgcgagacga tcagacagtt cggattgtgt
    18721 cgtccgcaaa cgacccccac ggtggggcgg aacgcgacgt caccgtgccg ctgaacgttc
    18781 tgttccccgc gcgcaccggc tccccatatg cgcgtgtgag tagcgagttc cccaagtcct
    18841 ccccgcgtca ctgggcgtcg tacggactgg gcgcgtatac cgtgttaatg gcagagcacg
    18901 ttgtgggttc gttcccctgt ggcgcaacca tcgtggtcca ctcgaacgtt cctctgggcg
    18961 cgggagtgag ctcatccgca gctctggagg tggcggtaat gaaagcgctg tcgcacgcgt
    19021 acggctttcc gatggcaggt attcctcttg cgtcactgtg ccagaaagtg gagaactacg
    19081 tcgtgggggc accgtgcggg atcatggacc aggtcacatc tgtcctcggc cgtgagggcg
    19141 agttcctttg cctgaaatgc cagccccacg acgtgcaggg ctttgtcaga ttgccaaaag
    19201 gcgttcgggt cgtcggatta aactccgacg tgaagcacag tgttggagga gaacgctaca
    19261 caagtgcgcg ggtcggcgcc tttatgggcc agcgaataat tcaggaggtc gggctgcgcg
    19321 aggccggggt tgatcccacc ggcggctacc tcgcgaacat cagcaaggcc gatttccgca
    19381 aaaaatgggc gcgccatctt cctcaaaaaa tgaccggagt caagttcctc gagttgtacc
    19441 aacgcacgaa cgacccggtg gcgcccgtgg ctccgcaaac cgaatacatg gtgaggagca
    19501 gagcggcaca tcctgtgtac gaaaacgata gagtgcagcg tttcatttct ttgatgaaga
    19561 ctgcgacgga ttcgggtgaa aaggctccat tgatcgctgc ggggaagctt atgtacgcgt
    19621 cccattggag ctacggccag cgttgtggat tggggagcag ggaaacgaac acactcgtga
    19681 gtctcgtgcg ccagaggggc gtttccgagg gatttttcgg cgcgaagatc accggtgggg
    19741 ggtctggcgg cacggtcgca atcctgtgcc ggcgcgatac agatgccgct ttggagagta
    19801 tcgcagaacg ataccatgag atttcagggc gttcccctca gctattcctt cgttccggcc
    19861 ctggagcaga tcaatgggga gtgatccagg tctctttctg aaaaaggtgc aatcgtgcgc
    19921 gtcgttgcgg gaacagcaag gagcatacct cttttctggg tgaagtcacc agacacccga
    19981 cccatgactg atcgggtcaa gacttcgttg tttgattcgc tgaacccgta cctgctggat
    20041 gctctggttc ttgacctttt tgccggtgtc ggcggcctcg ggatcgaggc cctcagccgc
    20101 ggagcagcac actgtacgtt tgtcgagcgg gaccatctct gcgtggaggc acttcaaacc
    20161 aatctgcgca aaacacggct ttctgaccgc gctacggtgt acgaacggga cgtcgaaaca
    20221 gcgctcacgc tgatgcgatc cgaggactct cccgttaaca ttgtcctctt cgatcccccg
    20281 ttttcgttgg ggaaacctcc ggcgaggacc gcactggaag cgctggtgga aaggttggcg
    20341 gacggattca tcgaacgggg aggtcttctg gtataccacc acgacaacga aaccgaggga
    20401 ggattcaaga cccgtcgctt cggcgtcatt aacacccgaa aatacggtcg aaatcgcgtt
    20461 acgatcctga aggccctctg aggttttcct cgcgaacggg cgccctcccc gaaagaacga
    20521 agatgggccc gacggtggta agctccacct cctcgaaacc aatcgcctcc atgaagccgg
    20581 ctgtttcgtc gggccgcgtc ggaatgtcca gccgttccgc catgccgaga aggtccgcct
    20641 gactggcaag accatccaga tcgttgacga gtggcgtcca ggcgcacgag aaagcccggc
    20701 ccggccccag caacctcagg attccttgca gataatcgga aaatcccgga atacggccga
    20761 ttccaaacca cgatatcact gcgtcaaaca attgttcccg gaacggcgga ttcagacagt
    20821 cgccaaccac gaacacaaca ttgtcgtatt gtgctcgctc gatctgtccc tgggcaaccc
    20881 ggagattgag gaatgccgta tcgaagccgc aaatcatcgc gtcctgcggc gcgcgttcca
    20941 cgagcggcac aagtgccccg ccccagccag tgcccacatc gagcaccgtg tctgcgaaat
    21001 tgacttccgc ccgcaaccac atctcttcca tcgcatccaa atgcgctgct atggcattct
    21061 gttgctgctg gacaagacga ttccgggaaa aggtctccca gaatgattcg ctcgcgcgat
    21121 agcgcccctc gcgcacttcg gcggccgtca ccgctggttc gaacgcacgt gtcgccggat
    21181 actcgacggt gccatccgca agcgactgaa ctgccttcga aaagcccacc ttctgcgcct
    21241 ctcgcccaag tgtcgccgca aattcgtcca cggcgcnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnaagc ccaccttctg cgcctctcgc ccaagtgtcg ccgcaaattc
    21421 gtccacggcg cgccggcccg gaatgagcag tataggtatg gttgaatgga cgggaaaagt
    21481 tccgccgcag gcgtggcacg cgagcgttcc ttcgacgatt tcgccgccat cctcttccgt
    21541 cacattccat acagatatct gctcgcggca ccgcgggcac cgaagcttct ccagaatcgc
    21601 ttccttcatt tgcactccga acaagacgca aaaggggggc gtgagtgccc cccggaggtt
    21661 ggatgtctag gtttggcgca ctcagggttg ctcgccggct cgtaacgtac ggatggtcac
    21721 tgagcgtatc acgacgggcg tcagcggatt atcacgagca tcgcgcggga cagacactat
    21781 cttgtccacg acctccatcc cgcgtacgac atttccgaac accgagtacg caccgtcaag
    21841 gaacgtttga ggggcaacac agatgaagaa ctgactcccg cttgaccggc gctcgggatt
    21901 cacctgatcc gcctgtcgag ccgccgacac cgctccacgc aggtgaagcg ccttgatctc
    21961 tgcgggaatc gtgtaaccgg gacctcccgt tccgtcgttt tcgcggtcgt tatccttgct
    22021 gttgggatct ccgccctgaa tcatgaatcc agggattacg cgatggaacg tggtgccatt
    22081 gtagaacccc tgtcgagcaa gtttccggaa attcgcagtg tgccggggag cgtcctggtc
    22141 gttgagccgg aacacaattt caccgagggt cgttttgatc accgcgacgt cgtacgtggc
    22201 ttgttcttgt gctgtcgcag tttgagcagc cgcagttgcc gtttcgacca cgtcactcag
    22261 tgcttcctgg tttccagccc gtgccttgac ctggaagaaa taggtcgttc ccggttccag
    22321 cccttcaagc ggagttctgt ggagtgtcgt gattgcaggg acagggatcg tgcgcgaaag
    22381 ggtctcccgg gtggttccca cagcgatctc gccgtctgtg ggaatggacg tggaccagaa
    22441 aacgacgacc ctgtccggtc ctaccgactc tatggtgata ttctcgattc tgggcgctga
    22501 ggcttctttt tcgccggatt ttccacagga catgagactg agaagaggcc acaacaggat
    22561 cagcaggctg attctcgagc gcatggcaga acgtactccc cgcttgagtg ttttctcaac
    22621 gcgtttcccc ggattttgcc ggacgcaatg ttactttgtt gattgtaacc ggtgtgatgg
    22681 gattatctcg gtcatctcgt ggcgcagcga cgatcttgtc tacgacatcc atgccgctca
    22741 gaacctctcc aaaagccgag tacttcccgt ccaagaatgg tgcatccgcc acgcagatga
    22801 agaactgact cccgcttgat ctccgttcag ggttgacttc atcgcccatt ctcgctgcgg
    22861 caagagttcc ccgtttgtgc ggcatattta tctccgccgg aatctcatac gaaggcccgc
    22921 ccatgccgtc atttgccctg tcggcatctt tgctgttcgg gtccccgccc tgaatcatga
    22981 aaccggggat cacgcgatga aacgtagtcc cttcatagaa gccttctcgc gccaacttct
    23041 tgaagttctc cacgtgtctg tgggcatcct tgtcgaaaaa cccgaccact atgtcgccgg
    23101 cagaagttga aatcaccgcc acttcccgcg atttcgaccc gcaggaggtg agaatgacag
    23161 acacgacgac aagaggaaga gtggcaagga agctagttct taagcgcaca cgaacctcct
    23221 cccgccatac agcatgaatc gcacgccgcc gccttggaac tggaagcacc cgcgttgact
    23281 tgagcggcaa aaacgctcag cttcttcttc acgttcttgt tcccgcaatc acacgcaacc
    23341 gactcattcc ggtcatagat caaacgctca aaatctgctc cacacgaatc gcaatggtat
    23401 tcaaagatcg gcatgtttct gcctcctgtt attgaaaaac ccctacattc ctataccaag
    23461 ataacaccgt cacgggtcgg tcagttcatc tacgctgacg caatggagcg ccatatcgac
    23521 acgtccctgg gaatcaaagg caacaccctc tgaaagcagc ttctcccttt gttccgtggg
    23581 cgtttcgaag gcccacgctc cggtgagaac cccgttccga ttcaccaccc tgtggcacgg
    23641 gacatccgcc tccctgcaca cgaacagggc gcgaccgacc tgccgcgcgt cgcgcgggcg
    23701 gtccagcgcc agggcaacca gtccgtacga tgccaccatg ccgctcggaa tcctacgcac
    23761 gacgtcgtac acgcgccggg tgaagtttgc ggcgggaatc atcgggctgc aacattcgct
    23821 atttcgcgag cgtttagtgc acgcccgtag agcctgaaat cccgtatggc gccgttgaac
    23881 acgtcctgct ggccaaatcc gatgcgcaag ggaacatcat tcgtgatgtc aaatggtctt
    23941 ggagcgtggc gctgggacac cttcaccccg ttcacccaga ggctcagtgt gtttccctct
    24001 ttcaccgcag cgacgtggtt ccaaccagca ctcagttccg aatcgtgggt aacgcatgca
    24061 cccgcttcca tcgaatgcac atggccactc ggaagcactc ccgcgaagag cttcccctcg
    24121 tatgcggcca tcgaccacac ccgcctgtac cgaacgtcgg gagtccaatc gagccttccg
    24181 acggctgtcc attctcggtc gccgtcagat cggtacacct gtgccagcgg caggcttccc
    24241 gcgtacagct tgccgttgaa cacagccatt gccatcactt ccagttcgtc cgcaagcctt
    24301 ccgcagtccg cccagtcctt gccgccttcg tacctgtaca caacggcttt gggccatgtc
    24361 cccgcatgaa gggccccttg gtaaacggca aaagaataca cctggtctac gtctttctgg
    24421 aggccgcaat cgtgccattg cgatccgtca aaacgccata cgcccgctcg ttcgttgccg
    24481 gccgtgaaca gggaaccatt gaaaactccc agggcaaatg cacggcaatt ccccgggcgc
    24541 ccgatctcct cccagtgcga ttcgccgtcg taccggaaga cgccctgcga atagagcgga
    24601 atcgcgtaca ggctttcctg aaacacactc aacgcaaatg cctcgcaggc gtcgccaagg
    24661 cgaccacaat ccacccagcg ttgcccgccc tcgtagcggt acacctttcc cccggggaag
    24721 gtgttcggcg aatctggtag cgacgatccg gatgcgtgat aatgcgctgt tcccgcgtaa
    24781 aggcacccct gatgaacggc cagtgaggcg acgctgttcg cgccgtccgg gctcccgcaa
    24841 tcaatccagg cagaatcgcc ggcgtaacgg tacacgtggc cccgctcgtt a
//
LOCUS       DAOTNG010000005        24003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2185_length_24003_cov_5.928178, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000005 DAOTNG010000000
VERSION     DAOTNG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24003
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2185_length_24003_cov_5.928178"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="P5179_00545"
     CDS             <1..1525
                     /locus_tag="P5179_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS93740.1"
                     /translation="ELAKHALEEFSDLFPVTLSVLVLCKHSSDTIEVVLPAEPPIPGL
                     APGLPLPEAIAETSSAGPIIWEKDFGVPPARSGLVPLCVVLSARGARVGVILSFVARR
                     QLGEAMANEAAMRSYAAFVGMSLFNAQLQQHARMRDEAYRVWLKGQGLGIPPDRVPSA
                     SAAHRELTRRLAVIKKNPPAELGTFESLTGDSVAIQEVRKRLQQVAGTDTSVLLLGPS
                     GTGKELAARAIHGQSKRKSGPFVPVNCGAIPEGLLESELFGHTKGAFTGAYAAKSGVF
                     QRAHHGTIFLDEIAEMNPSAQIRLLRVLQERSVTPVGGETAVPVDVRVIAATHQDLGV
                     AVAEGRFREDLFYRLNVFPIHLPTLAERMEDIPALVAALLYKISLKTNLPLTGMAEDA
                     VERILAHPWQGNVRELENCLERALLLAGGEIIRSVHLQFDTAGPGTTLIARNSTEAPG
                     GRKLADVEQELIEETLRECNFNVSEAARRLGISRDILRYRIKKLNISREKIRRDTAQ"
     gene            1555..1725
                     /locus_tag="P5179_00550"
     CDS             1555..1725
                     /locus_tag="P5179_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93741.1"
                     /translation="MNGQILDQSCCLWISRGVISYGTGFPKAARSVTAGVSSVEYLYE
                     ETIADLMTTVAH"
     gene            1982..2914
                     /locus_tag="P5179_00555"
     CDS             1982..2914
                     /locus_tag="P5179_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRS93742.1"
                     /translation="MPRGGADLIPGEQSGAIAFGSHLSLDRPGAGERAAVVDRFRGLV
                     YRIYRDFGRDLPWRRTCDPYEILVSEVMLQQTQVGRVAQKYPEFLRAFPSFHDLYEAP
                     LSDVLKVWQGMGYTRRAVALKRIAERVIRDYDGRLPRSPEHLQTFPGIGAATAGAICA
                     YAYNLPTVFIETNIRRVFIHTFFGEHETVSDKQILPLVEETLDHSQPRVWYYALTDYG
                     AALGRRLENPNRKSAGYRRQGRFEGSKRQIRSTIVRELLARGALTLGDIDQLCSGTPF
                     EGGSILRELQRDGLVQPLGDQWCVAFTPDSAGTA"
     gene            complement(2915..3949)
                     /locus_tag="P5179_00560"
     CDS             complement(2915..3949)
                     /locus_tag="P5179_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93743.1"
                     /translation="MSYERGIQAIRLQMPDTIPHTEYVSNPTLVKHVTGIDPSTPGRE
                     GEAWKQFMIRLNYDLVWRTDSPPFEKAPHAWMGSARYSETQELKPATYPFANEEEVLS
                     FDPVAAIGVPDRQETRQLFERNWRATQSDLPFLVVPSGYYNTVFTWCIVTFGWELFMA
                     AAMADPKRFDRVLEGFYQLSRPIFEAAAEIPLPAFICHDDIVWTAGAVFHPDWYRRFI
                     FPRYKKLWAPLREAGIPILFCSDGNFTEFVPDIADAGANGFIFEPLTDLEMVAARYGN
                     THVIIGNADCRILTYGSRGDIYAEVKRCADIGRSLPGYFFAVGNHIPYNVPVENAMYY
                     FDLIEELGRR"
     gene            4133..5626
                     /locus_tag="P5179_00565"
     CDS             4133..5626
                     /locus_tag="P5179_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93744.1"
                     /translation="MRSTPIGIVMLNDEREHVWRMNAAVNMSVVRKWAQIIRKGVKNV
                     DGSAPEVVIGSEIVTGVRSAQKVGEELRRANVKQLILCYNVWNFPFLVWPLLNSVGRD
                     VPVLSLSNNNGEFPGNVGLLATDGALRQAGIRTHRIVGEMDDPKTCQAVFDWVRASQA
                     LTTMRNEVFGIYGGHSMGMETGFFHLVPTVKTFGTTGRQIDQLWLVHAMKEVDDSEVE
                     KGFKWLTEMLGKRILYDGKMLTPETLKTQIRLYCAMKRVNEEKGFDFCGLKGQRELTE
                     YLCLGDVPEMIMNDPYDWNGPKEPTVCATEADAYAAMTMQILKYITGGLPTLFMDVRL
                     YLPEKNLWDWCNSGNHSSWYAAQSSDPKKNYAKIHFHPALEMYFKAGGASVEFDAKPG
                     EMTFCRLGLWDDKPFMVITRGNALDLPAAERKAINKATNPTWPHVHARIQGSFEEFLS
                     VFPCNHVLGAPGNLVQPLVYLCEIAGIPPIVLGEEGAKRIQPIWERI"
     gene            complement(5731..7065)
                     /locus_tag="P5179_00570"
     CDS             complement(5731..7065)
                     /locus_tag="P5179_00570"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRS93745.1"
                     /translation="MEHADASYVVKQAKDHKVKFIRLWFTDVLGFLKSVAIVVDELED
                     ALVDGVGFDGSSIEGFARVDESDMLAVPIPTSFQILPWRPQSDNAVARMFCEVRQPDG
                     TPYEGDPRFVLQRALKRAHDMGFTFYVGPELEFFYFKEDQPPPKVLDRGGYFDLTPLD
                     IASDLRRDTIMTLEQVGIDVEYSHHEGAPSQHEIDLRYADALTMADSIMTTRLVVKEI
                     AGKHGIYASFMPKPLVAFNGSGMHIHMSLFRGDSNAFFDPGNPEHLSATGEQFVAGIL
                     RHAAEFTLVTNQWVNSYKRLKPGFEAPTQIAWAYRNRSHILRVPSIKPGRSESMRIEL
                     RSPDVACNPYLAFAVLLHAGLEGIERQYKAVPPTESSPEQLSDSARRRKGIKQLPDSL
                     NEAIRLAEQSELVRRALGPHIHAKLIENKRMEWRKFAEHVTDYELDRYLPLL"
     gene            complement(7074..7742)
                     /locus_tag="P5179_00575"
     CDS             complement(7074..7742)
                     /locus_tag="P5179_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS93746.1"
                     /translation="MPCVLIVSNQQNARSAIAEDVEEIGIEAITTDDLSRALDILETR
                     HPSLVLLDLIDRTLGAESLWRQQMRQAPKDLPFLLLIPEEGIRNFDFPPQTKDFILVP
                     FVRAELHARLRLALGSLLSTQASNVIERGQLRIDLDRYKVSVANQPVELTLKEFELLR
                     FLAEHPGKVHTRESLMTRVWGYDYFGGTRTVDVHIRRIRAKLEPHADEYIDTVRGVGY
                     CFRD"
     gene            7963..9054
                     /locus_tag="P5179_00580"
     CDS             7963..9054
                     /locus_tag="P5179_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93747.1"
                     /translation="MAFRVAIIGTGHVAHLHANAYRASGSAEIVAVADVNDEVRAAFQ
                     REYGIPNTYGDYRAMLDSECPDVVSVCVWPHLHPEMTIRAAGTHGVKGILCEKPLALT
                     LPAMNEMIAMCRQKGIALGVGHQLRHQARVRAVRELISSGTFGAVRRVWAVCGSGDLM
                     SNTIHTIDLVHFFCDDRQVTRVFAQVDPGDGAYRFGHPVERTSVAYALIPEDGGIPEV
                     RICVESANCWSQGYHYITIECADGTIEINKPGSPPVIAKGWRSAEEELKELQEEPDPS
                     TLAVRDLFDAVERHREPVSNGITGYKAVEFILAIYQSALKRSIVELPLPLDTPDPLRH
                     FFNWDGVIRGGRGTAFSTTGAVTTTGKIS"
     gene            complement(9047..10948)
                     /gene="asnB"
                     /locus_tag="P5179_00585"
     CDS             complement(9047..10948)
                     /gene="asnB"
                     /locus_tag="P5179_00585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRS93748.1"
                     /translation="MCGIAGIWHRDGRPVSPQMISAMTDVQSHRGPDDRGTYVSGDLA
                     LGHRRLAIIDLTETGHQPMSNRDGTLWITYNGEVYNYVELRAELEGRGARFRSTSDTE
                     VLIAAYEQWGESCVERFNGIFSFAIWDSRRQTLFCARDRFGVKPFHFAEIGPTFLFAS
                     EPKGVLASGIVPRGPDWRVVYRYLVLNLLNVDNNTFFSSIQKLPPAHTLTVHRDGGSV
                     LRRYWDIPTIQSGEQRSDAEWLEEYEATLEDSVRLQMRSDVPVGVCLSGGLDSTSITR
                     IATEFSSIPTKTFTAFLPHPLYDEREPVSAFTRLMNGKTEPHFVSPELDTWVHELPKV
                     VYHHDEPHAGYIAATQWEVMRLVHQRGVKVVLDGQGSDETLGGYNFFVESAAADLVRS
                     GRVLDAFRVLSLLEDRRGTGYAAGARRLIKVAGRAFFSRDHLFEIEGKWNAAGLPLSR
                     EWCSRFRRFDIPTIETTGSRLEDDQRTAMFVTMLPYLLTFEDRASMAFSIESRVPFLD
                     HHLVETALRLPRHLRVDGTTTKVALRRIMEGKLPGEIVNARLKRVFGTPYEHWFSEKL
                     SGFARDILTSQAFLERPFIEKRKVPGLIASFAAAARSRRKAFNIWNLINLELWFRVCV
                     EQNADIVPN"
     gene            11282..12526
                     /locus_tag="P5179_00590"
     CDS             11282..12526
                     /locus_tag="P5179_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRS93749.1"
                     /translation="MRKWFWSGIVAITAAAANVGAAGFALYEHGAAATATAGAFIARA
                     NDPSAIYYNPAGISGQRAGVMLGTTLITPSGDFAGANPFPGTGYTAKQKDALFFPSHL
                     YVASPLGPVTVGLGVFNPFGLGTNWPEDWKGRDISTKTEIQTYYFNPVVAYSVGPVSM
                     AAGMQAVYGSLELNRKVKHPLLGDDVAAMHLEGTSDINYGFNAGVQFRPMPMLTVGAS
                     YRSQVDSKVNGTAAFDSMNAKWTMVKTSLPAKADVSTTMPYPAVMGIGVAVTPIPDLT
                     IEANVVQTQWSTFDTLTIAFAAPYSALTEKTPELYSNARSYRIGAEYRLPAWSLALRC
                     GYLFDESPAPPMAVTPLLPDADRDSFQVGAGYTMGKTTFDFAYMYLKFKERSTEGLNP
                     KNFNGTYNTTANLFGFSVTHRF"
     gene            complement(12973..13830)
                     /locus_tag="P5179_00595"
     CDS             complement(12973..13830)
                     /locus_tag="P5179_00595"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRS93750.1"
                     /translation="MDIDGTTRVVGVIGDPVSHSLSPAMHNAAFKALGLNWVYVAWHV
                     VPEGVEAAVNAVRGLCLPGMNITVPHKKAVIQFLDELLPAAKAVGAVNTIVNKDGQLL
                     GDNTDVYGILQAIRVGAKMDTVPPVVVVLGAGGAARGIVYALTTLPEVKSIRILNRTL
                     ANAEELAREFGATSSVTAFGLEPHAARHALDGCGLLINATNLGRGKLANISPLPDAWN
                     CIPPRIVCIDSNYSPATTRLMEQVVAAGGVAFNGLDMLVHQGARAFELWTGMMPPVEV
                     MRAAANARL"
     gene            complement(13864..14592)
                     /locus_tag="P5179_00600"
     CDS             complement(13864..14592)
                     /locus_tag="P5179_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS93751.1"
                     /translation="MFFGSQDFFILLLIAFGAAVVQGTLGFAFGMVAMALAALVIDVR
                     TASILVSPLAFVNVSTTLWSVRRSIRWKLVLPVVGGTLVGIPFGLAILLGGNLILIRF
                     LVSALLVYVGVTSLIGFKVRRHALPGWVAAVAGLGVGVIGGATNIGGPPLVAYASRQP
                     WDAETFKATLLTTFFVSSGTKSALLIANGAIDARMLACIAGLVPAIIAGSLIGVHLFN
                     RINKERFKRIISWTLLVLAVLVQV"
     gene            complement(14601..14924)
                     /locus_tag="P5179_00605"
     CDS             complement(14601..14924)
                     /locus_tag="P5179_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93752.1"
                     /translation="MRHVSLYDLPDGNDQAELLANLPASYGSFGASVRFFARGEATEA
                     QRASNGVLLLVAQGSCVVRVDETEYPAARFDLFALESGETFEICADEIDPAVVLRIDA
                     GNSEG"
     gene            complement(14975..16030)
                     /locus_tag="P5179_00610"
     CDS             complement(14975..16030)
                     /locus_tag="P5179_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93753.1"
                     /translation="MSRKIRVAVTGAGGEARWYMEAYKTCPDSELVLVQDIDPAKARE
                     QGERLNVNWTTTFEDLLGPDIDLVDVSTPNHLHAQQTIAAHEAGKHVICQKPMAPTIR
                     ECDEMIAAAQRNNRKLGLYMSSLGAPLFHEIKEMIRQGFFGIISSVSSRGAHRGGYEA
                     KDNTAWRGSAEKTGGGVFIQLAVHSINLFQWLLDDDIVQVAALSTNRLCKHSIGGDDL
                     THAVAEFRGGVYGSFEAGYAAEGGLFALYGTKGSLVMTAGQTFLWSELPWDGDIIKYT
                     PRGGKEMTVLDPADYGPRQARFAGKYDQHAAFVRAILEDAPVPVPGEIGRRDVAIVKA
                     VYRSAEERRHVLIEEMG"
     gene            complement(16106..17530)
                     /gene="melA"
                     /locus_tag="P5179_00615"
     CDS             complement(16106..17530)
                     /gene="melA"
                     /locus_tag="P5179_00615"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS93754.1"
                     /translation="MTKVAMMGAGSAGFCRMLVEDILSFESMKDIHFALMDVDPERLD
                     LVYRSMLRMKEEHTLACTFSATTDRREALTGADFAISMIQVGGLEPYKLDVSIPLKYG
                     IDVCVGDTVNPGGIFRGLRHVPALLDMIRDMQDVSHPEIIFMNYANPMAIVSWAMQKM
                     YPDTTSVGLCHGVQHTTNMLCQWLGVAVEECDVLTAGINHMAWFLKFRHNGSDLYPRM
                     WDKLAAEGPIRGEEYRFEMMKATGYFMTESSGHLSEYLPYFRKRKDLLELFNGPGFSG
                     ETAYYLNSCLQHRDKYLEEMRELADGRKPVPFRRGYKSVEYASNIMNAKLTGEITRFA
                     GNVLNKGFITNLPYDCCVEVPVFVDRLGLHASYVGHIPEQCAALCQSNISAQELAVKA
                     AIEGDPEAVYHACMLDPLTAAVLAPHEIRNMVDEMLEAQAPWLPQFKGKSSHAPGHRV
                     GRLQTGAKSLVVGAEVSSKKGHYD"
     gene            complement(17580..18608)
                     /locus_tag="P5179_00620"
     CDS             complement(17580..18608)
                     /locus_tag="P5179_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRS93755.1"
                     /translation="MDTYSFAPELCHEYLMDRAPLALTFDPHKHSDAESFDAWRRKVG
                     AKLRQLLGNTPAKVPLNIRHEFETQHEEFVERRFVFTAEKNADVPCHLLLPTHGSAPF
                     PVMICLQGHSTGMHISLARPKYEGDQKSISGGDRDFAIRAVKEGFAALVMEQRCFGER
                     KDAKHEGSRCHHATMVSLLLGRTMIGERVWDVSRAIDALATFPELDSDKIACMGNSGG
                     GTITYFAACCDPRIKAAMPSCYVCTFRDSIGRINHCEDNYVPGILKWFEMGDLACLIA
                     PRPLVVVAGKEDEIFPIKGVRSTFRRIRDIYSAAGHKDKCRLVVGNGGHRFYADDAWP
                     RFKQLTRW"
     assembly_gap    18806..18815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18816..19698)
                     /locus_tag="P5179_00625"
     CDS             complement(<18816..19698)
                     /locus_tag="P5179_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93756.1"
                     /translation="MRERILAVIRGNPHDRVPFVQYDGLAAPNEVVWDVVGRANMGII
                     RWSSAYRIEAPDCVITHEPVEINGRTGERARLITPRGSLVQERVFEPAYQSGATRKHY
                     VCDSADYEILLSYLRNVVVVDDATPFLLDRDALGDDGVPMVAVHRTPYQQMWIQWVSL
                     EQLAVDLEDDLPVLRECLEVLGNIERRIFESVCRATREHGVPFVDFPDNITAPAIGER
                     YFRGYCVPFYDELAGMLRDDDAPVFVHMDGDLKPLWEAIGGSGVRGIDSLSPPPDNDT
                     SCADAATRWPDMRLFVNF"
     gene            19849..22500
                     /locus_tag="P5179_00630"
     CDS             19849..22500
                     /locus_tag="P5179_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS93757.1"
                     /translation="MRSMTGCIQTRLVPSILCVVIVMSANPASATMNLNGSFEDGAES
                     PSGWTRHPGGFLGEDGHTGARSIGGRARTKATVWTSDVMEVEAGQSWRISGWFRVKKG
                     LVFLRTEFLNSAGAVLGSADTPEIAKDRHASGWRYTAVETDAPASAEHVRMSLIVRGE
                     ALCDDIVMTPLIANLLYNPTFEAGSRGRVTFWDPDPVLTRATAKTGSGIADETGGRNG
                     AGLRIRSDSSWWAMRNIQQPIPVGLTTFRLSGWARWEGEAPDIAIAWTDNGGVLHGLS
                     PATPRETVNRWTRFEVSDAHPPVQAETMTVSLVGNIGTAWFDDFQLEPVAPATNLRRV
                     VKVHVNQVGYERNLPKSCVVATNFVPHGGRNFIEVHGEDGHTLGSFPLVGPERIHNGM
                     PDDWGEYFWRADFSSLKETGRCTITAVVGGVRGESYPFLIGDGILFGGTAPLGVEFFF
                     VQRCGFAVPGWHAACHLDDALLPDGTHIDAVGGWHSAGDYNKLMYENGDGGVLYALTE
                     AFRTKQQVFEASDRDNDGVPDILDEARWGADFVVKMQVPETGGLRKDVSQGPGRTWMK
                     WCPPEVHTDNIIGTDDDPVILPGEGNSPLAIGGWMRLAGELERRGEDGSKYGSSAERL
                     WGYSTQNGETGFSPLNLLSALDLHAATGERRYAEFASRCVDTLLASQVPSGRHAGAFG
                     AFGEHAAGALGTYALKFPEDSRIPRIRAALARYMEFCLTTAENPFGLAKQSVGEQDFF
                     FEPTSTLGHNFEILARAWSAIIAYQVTGHEAALTFALDQIDWVMGKNPYGLCMMEGAG
                     SNNPPRYHHRYDSIPGRERGAVPGAIPNGFVRTPRALDQPGFDMSRSRTERDHPSYRT
                     SEPWLVHNMWHLLAVSALQRVVDERAR"
     gene            22668..23543
                     /locus_tag="P5179_00635"
     CDS             22668..23543
                     /locus_tag="P5179_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93758.1"
                     /translation="MDNIFRNKMVQGIGLGIAICIVVVGAFFGIKSLMGPGSLKEQLT
                     GQKKYQPPSSASTDSAFAFPTIFMRVVGDSVRGLVSIDPTKKIDAGTELGVQEMVNRL
                     RVGHQTLAQNPGPRGVPNAFNLVLDKSSTYGQAMLGIYIGVNGTFQMPFLITAEGNQH
                     SVEPPRMHPQFGPMGWAAWIWWLPDQRIKWVVWPDSLEGNFTFHVLPAKNGKPDIEGL
                     RGKLRTAMDGKAHIPPPQQRLIGFWGYDSLPYESAIQFFDVLRYPKTGEPLCSRLTLM
                     EGEMMSPPADSVCVE"
     gene            23628..>24003
                     /locus_tag="P5179_00640"
     CDS             23628..>24003
                     /locus_tag="P5179_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93759.1"
                     /translation="MTTITVGLLGAGRGLSLAQLFDRVPGYRVVAVCDAIPERLDRAR
                     SACPRAEGFRDYAAMLREPFDVMIVASAPPSHADHVCAALEAGKAVLSEVPAAHTLDD
                     AQRIVDTVARTSGYYMLGENCNY"
BASE COUNT         5128 a         6790 c         7061 g         5014 t
ORIGIN      
        1 tgaacttgcg aaacatgcgc ttgaagagtt ctccgacctt ttcccggtaa ccttgtcagt
       61 gctcgtgttg tgcaagcaca gttcggacac cattgaggtc gtgctgccgg cggaaccgcc
      121 gattcccggc ctcgccccgg gtctcccttt gcccgaggca atcgcggaga cgtcttctgc
      181 tggcccgatc atctgggaga aagacttcgg cgtaccgcct gctcggtccg ggttggttcc
      241 attgtgcgtg gtactgagcg ctcgtggcgc ccgagtcggc gtaatactga gttttgttgc
      301 acgccgacaa ctgggcgaag cgatggcaaa cgaagcagcc atgcgatcat acgccgcttt
      361 tgtcggcatg tcgcttttca atgcgcaatt gcagcagcac gcaaggatgc gagatgaagc
      421 gtaccgagtg tggctgaaag gacagggact ggggattccc ccggacaggg taccgtctgc
      481 ttccgcagcg caccgcgagc tgacccggcg ccttgccgtc atcaagaaga acccaccggc
      541 cgaattgggc acgtttgaga gtctcaccgg ggattccgtt gcgattcagg aggtacgaaa
      601 acgtcttcaa caggtcgcgg gcacggatac cagtgttctt ctgctggggc cttccggcac
      661 gggaaaggaa ctggcggcac gagcgattca cggccaaagc aagcggaaaa gcgggccttt
      721 cgtgcctgtg aactgtggtg caataccgga aggccttctc gaaagcgaac tgttcggtca
      781 caccaaagga gcattcaccg gcgcgtacgc tgccaaatcc ggggtgttcc agcgggcgca
      841 ccacggtacc attttccttg acgaaatcgc agaaatgaac ccatctgctc aaatccggct
      901 gctaagggtt ctgcaggagc gcagcgttac cccggttgga ggcgagactg cagttccagt
      961 ggatgtgcgg gtgattgccg caacgcacca ggatcttggg gttgccgtgg cggaaggacg
     1021 tttcagggaa gaccttttct accgactcaa cgtgtttccg attcatctcc ctactctcgc
     1081 ggagaggatg gaagacatcc cggcccttgt ggcagcactt ctctacaaaa tctcactgaa
     1141 aacgaacctg cccctgacag gaatggcgga ggacgccgtc gaacgaattc tggctcatcc
     1201 ctggcagggg aacgtgcgtg aacttgaaaa ctgcctggaa agggcgctac tgctggcggg
     1261 aggggagatc attcgatcgg tgcatctgca gttcgataca gcaggccccg ggacaacctt
     1321 gattgcgagg aattcgaccg aagccccggg cggaaggaag cttgccgatg tggagcagga
     1381 actcatcgaa gaaaccctca gggagtgcaa cttcaacgtg tccgaggctg ctcggaggct
     1441 ggggatatcc agggacattc ttcgttaccg gatcaagaaa ctgaacatca gtcgggagaa
     1501 aattcgccgc gacacagcgc aatagtattg cacctgagga ttccaatcaa agagatgaac
     1561 gggcaaatcc tcgaccaatc gtgctgtctc tggatctccc gtggcgtgat ttcctacgga
     1621 actggatttc cgaaggcagc ccggagtgtg acggcgggcg tttcgagcgt cgaatacttg
     1681 tacgaagaga ctattgcaga cctcatgacg acggtggctc attgattgca cgaaatgatg
     1741 atccccaccc cggggaaacg caactgtgcg cgtacgtgga aggctccttg ccgaaagacg
     1801 cggcgcgcgg tgtgttgagt catacggtaa ggtgccggta ttgtttcgcg aggctcgttc
     1861 aggtgtttga tgcgttgtca gtcgcagacg ggcttgcgcg acactacgag cgttgcagac
     1921 gcgaacacaa tcaagagcgt acatgcgctc tgattgactt tgaagaggta tgtgcagtcg
     1981 tatgccccga ggaggtgccg atctgatacc gggggaacag agcggcgcta tcgcatttgg
     2041 cagccatctt tcgttggatc gaccgggtgc tggagagagg gctgcggttg tcgacaggtt
     2101 tcgtgggctg gtataccgaa tttaccgcga tttcggacgc gatttgccct ggcggcgcac
     2161 ctgtgacccg tatgagattc tcgtctctga agtaatgctg cagcagactc aggtgggccg
     2221 tgtggcccag aaataccccg agttcctccg cgcattcccg agcttccacg atctctacga
     2281 agcgccactc agcgatgtgc tcaaagtatg gcaggggatg ggatacactc gcagggctgt
     2341 cgctttgaaa aggattgcgg agcgcgtcat tcgcgactat gatggacgac tcccgagatc
     2401 accggaacac ctccagactt tccccggtat tggcgctgcc accgcgggag cgatttgcgc
     2461 gtacgcgtac aacttgccga ccgtattcat cgaaacaaac atcagacgcg tgttcataca
     2521 cacgtttttc ggcgaacacg aaacggtttc ggacaagcag atcctgccgc tggtcgaaga
     2581 aacactggat cattctcaac cgcgagtctg gtactacgcg ctgacggact atggtgctgc
     2641 actcggacgg agactggaga atccaaaccg caaaagcgcc ggttaccggc ggcagggccg
     2701 ttttgaggga tcgaaacggc aaattcgttc gaccatcgtt cgcgaacttc tcgcgcgggg
     2761 ggcgcttacc ctcggggata tcgaccagtt gtgctccggg acgccgttcg aagggggctc
     2821 catacttcgt gaactgcaaa gggacggtct tgtccagcca cttggcgacc aatggtgcgt
     2881 tgcgttcacc cccgattctg ccggcactgc gtgatcatct tctacccagt tcctcaatca
     2941 gatcgaaata gtacattgcg ttttcaaccg gaacgttata ggggatatgg ttcccgactg
     3001 cgaagaaata ccccgggaga ctgcgcccga tatccgcgca acgtttcact tctgcgtaga
     3061 tgtctccccg tgaaccgtac gtgagaatcc ggcaatctgc gttgccgatg attacgtgcg
     3121 tgtttccata ccgtgccgcc accatttcca ggtccgtcag aggttcgaaa atgaacccgt
     3181 ttgcgcctgc gtctgcgatg tcgggaacaa attccgtgaa gttcccatct gaacagaaca
     3241 gaatgggaat ccccgcctcc ctgagcggag cccacagttt tttgtaccga gggaaaatga
     3301 atcttcggta ccaatccgga tggaatacgg cgccggctgt ccacacgatg tcgtcgtggc
     3361 agatgaaggc gggaagcgga atctccgcgg cggcctcgaa gatcggtctg gataactggt
     3421 aaaacccctc gagaacacga tcaaaccgtt tgggatcggc catggctgcc gccatgaaca
     3481 gctcccaccc gaacgtaaca atgcaccacg taaacaccgt gttgtagtaa ccgctcggaa
     3541 ccacaaggaa gggaagatcc gactgagtgg cgcgccagtt ccgttcgaaa agctgcctgg
     3601 tttcctgccg gtccggaacg cctatcgccg caacgggatc aaacgagagc acctcctcct
     3661 cgttcgcaaa cgggtatgtg gcgggtttca gctcctgagt ttctgagtac cgtgccgatc
     3721 ccatccaggc gtggggcgcc ttttcaaacg gtggggagtc ggtccgccac acaaggtcgt
     3781 aattcagccg gatcatgaat tgcttccagg cctcgccctc gcgccctggg gtggatggat
     3841 ctattccggt gacgtgtttg acgagcgtcg ggttgctgac gtattcggtg tgaggtatcg
     3901 tgtcgggcat ctgcagtcgt attgcctgga tgccgcgttc gtaactcatg gcgggtctcc
     3961 ttggttccgg aacggggttt cagcggcggc gaagcaaagt gtacgcgcca gaatggaatt
     4021 gggtcaaacg ggggttaacc ccgggcgccc gagtccttag attctatcac gaacgggaac
     4081 cccaaaagca cgacatacgc caattgcctg cgtttgcagg aaggagatga gcatgcggag
     4141 cacaccaatc gggattgtga tgctgaatga cgaacgcgag cacgtgtggc ggatgaatgc
     4201 ggcagtcaat atgtccgtag tgcgcaaatg ggcgcagatc attcggaagg gagtgaagaa
     4261 cgtagatggg tcggccccgg aggttgtcat tggctcggag attgtaaccg gcgttcgcag
     4321 cgctcagaag gttggcgagg agctgaggag ggcaaacgtc aaacagctca tcctgtgtta
     4381 caatgtgtgg aatttcccgt ttcttgtctg gccgctgctc aacagcgtag gacgggatgt
     4441 cccggtgctc agcctttcca acaacaacgg tgagttcccc ggaaacgtag gcttgctcgc
     4501 gaccgacgga gcattgcgcc aggcaggcat ccggactcac cgaattgttg gtgaaatgga
     4561 tgaccccaaa acgtgccagg ccgtgttcga ctgggtacgg gcaagccagg cgctcaccac
     4621 gatgagaaac gaggtattcg ggatatacgg tggccattcc atggggatgg aaaccggctt
     4681 cttccacctc gtgccgacgg tgaaaacctt cggtaccacc ggaaggcaaa tagatcagct
     4741 ctggctcgtt cacgccatga aagaggtgga cgactccgag gtcgaaaagg gcttcaaatg
     4801 gctaaccgag atgctcggca aacgcatcct ctacgacgga aaaatgctga cccctgaaac
     4861 gctcaaaacc cagatacgcc tctattgcgc gatgaaacgc gtgaacgaag aaaaggggtt
     4921 tgatttctgc gggctcaaag gtcagcgcga actgaccgaa tacctgtgcc ttggtgacgt
     4981 tcccgagatg atcatgaacg atccctacga ctggaacggg ccgaaggaac cgacggtgtg
     5041 cgcgaccgaa gccgacgcgt acgctgcgat gactatgcag atactgaagt acatcaccgg
     5101 cggccttcca acgctgttca tggacgtgcg cctctacctg ccggaaaaga acctgtggga
     5161 ctggtgcaat tcggggaacc attcgagttg gtacgccgcg caatccagcg atccgaagaa
     5221 gaactacgcc aagatacact tccatcccgc cttggagatg tacttcaagg caggcggcgc
     5281 gtcggtggaa tttgacgcga aaccaggtga aatgaccttc tgccggttgg gattgtggga
     5341 cgacaaacct ttcatggtga taacccgcgg caatgcgttg gaccttccag cggcggaaag
     5401 gaaggcgatt aacaaggcaa ccaatccgac gtggccgcat gtccacgcgc gaattcaggg
     5461 cagtttcgag gagtttctca gcgtgttccc gtgcaatcac gtgctcgggg cacctggcaa
     5521 tctcgtgcag cctctggtgt acctctgcga aatcgcgggg attccgccaa tcgttcttgg
     5581 cgaagagggt gcaaagcgga tccagcccat ctgggagcgc atctgaagac cctgctgcgg
     5641 tcaaaaatac ggcgggcgcg gttcaggaga accgcgcccg ctgtatattc cgcgctggcg
     5701 taacttgctt gttgtgaccc cggctgaggc tcaaagcaac ggcaggtacc tgtccagttc
     5761 gtaatcggtt acgtgttccg cgaacttgcg ccattccatg cgcttgtttt cgatgagttt
     5821 tgcgtggatg tgcggtccga gcgcgcggcg caccagttcg ctctgttcgg caagccgaat
     5881 ggcttcgttc aggctgtccg ggagttgttt gatccctttc cgtcgacgtg ccgaatccga
     5941 gagctgttcc ggactgcttt cggttggcgg aaccgcctta tactgtctct cgatgccttc
     6001 cagacctgcg tgcaggagga cggcgaaggc aaggtacggg ttacatgcca catccggcga
     6061 gcgcaactcg atgcgcatgc tttccgaacg gccgggtttg attgagggaa cgcgaaggat
     6121 gtgagaacga ttgcggtacg cccaggcgat ttgagtcggc gcctcaaatc ctggtttgag
     6181 gcgtttgtac gagttgaccc attggtttgt cacaagggtg aattcggccg catggcgcag
     6241 gataccggcg acaaactgtt ctcctgtcgc tgagaggtgc tccggattcc cgggatcgaa
     6301 aaacgcgttg gaatcccctc ggaacaacga catatggatg tgcatgccgc tgccgttgaa
     6361 tgcaaccaac ggcttcggca tgaaactcgc gtaaattcca tgctttcccg cgatctcttt
     6421 cacgacaagg cgcgtggtca tgatcgaatc ggccattgtg agggcatccg cgtaccgaag
     6481 atcaatttcg tgctgcgatg gtgcaccctc gtggtggctg tactcgacat cgatcccgac
     6541 ctgttccagc gtcatgatgg tatcgcggcg caaatcggac gcgatatcaa gcggggtgag
     6601 gtcgaaatac ccgcccctgt caaggacttt cggcggtggt tggtcctctt tgaagtaaaa
     6661 gaactcgagt tcgggaccga cgtagaatgt gaatcccatg tcgtgcgcac gtttcagagc
     6721 tctctgaagc acgaaacgcg gatcgccttc gtacggcgtc ccgtcaggct ggcggacctc
     6781 acaaaacatg cgggcaaccg cattatcaga ctgcgggcgc cagggaagta tctgaaaact
     6841 ggtcggaatg ggaacggcga gcatgtcgct ctcatccacg cgcgcgaagc cttcaatcga
     6901 ggagccgtcg aaaccgacgc cgtcaaccag cgcgtcttcc aactcatcaa cgacgatcgc
     6961 gacgcttttg agaaaaccca ggacgtcggt gaaccagagg cggatgaatt tgaccttatg
     7021 gtctttcgcc tgtttgacga catagcttgc atcagcgtgt tccatggccg tccctagtcg
     7081 cggaaacagt atccgacacc tcgaacagtg tcgatatact cgtctgcgtg aggttccagt
     7141 ttcgcgcgga ttctccgtat gtgaacatcc acggtgcgtg tgccgccaaa atagtcgtac
     7201 ccccatacgc gagtcatcag cgattctctt gtgtgcactt tgcccgggtg ctcagcaagg
     7261 aaacggagca gttcgaattc cttcagggtg agttcaacgg gctggttggc gacggacact
     7321 ttgtacctgt cgaggtctat tcggagctgc ccgcgttcga ttacgttcga tgcctgcgtt
     7381 gacaacaatg aacccagcgc aagtcgcagc cgggcgtgaa gctccgcccg gacgaacgga
     7441 accaggatga aatcctttgt ctgcggcggg aaatcgaaat tgcgaatgcc ttcttccgga
     7501 atgagcagca ggaatgggag gtctttcggc gcctgtcgca tctgctgtcg ccaaaggctt
     7561 tccgcgccga gtgtccggtc aatcagatca agaagcacga ggctcgggtg cctcgtttcg
     7621 agtatgtcga gcgctctgct gaggtcatcc gtggtgatgg cctcgatgcc gatttcctcg
     7681 acatcttcgg cgatcgcact gcgagcgttc tgctggttgc tgacaatgag cacgcatggc
     7741 atgggaggca cgttcctggg cagaactgcg tgatttgttg cgtgatttca aagtagtcac
     7801 gctcgaatat gtactatcgg tctcgcggag actctgtgca agggcgcctg cgcgcggcaa
     7861 agaagtgcag gacaccggtt cacttgccga tgaagagaga ttttctgaac ttcaaacacc
     7921 tcccggcagt ggttatgacg acgcacaaac gaaggagtgg cgatggcgtt cagggtggct
     7981 atcatcggga cagggcacgt tgcgcatctc catgcgaacg catatcgtgc ttccggcagc
     8041 gcggagatcg tggcagtcgc agacgtaaac gacgaagttc gcgcggcatt ccagagggag
     8101 tacggcattc cgaacacgta cggcgattac agagcaatgc tcgactcgga gtgtcctgac
     8161 gtcgtgagcg tgtgcgtgtg gccgcatctt catcccgaaa tgacgatcag ggcggcggga
     8221 acgcacggtg tcaagggaat cctttgtgaa aagccgcttg cgctgacact cccagcaatg
     8281 aacgagatga ttgcaatgtg caggcaaaag ggcatagcct tgggggtggg gcaccagctg
     8341 cggcaccagg cgcgcgttcg agcggtacgg gaactcatca gctcaggcac attcggcgcg
     8401 gtccggcgcg tctgggccgt gtgcgggtcc ggggacctga tgagcaacac cattcacacg
     8461 atcgatctgg tgcatttctt ctgcgacgac cgccaggtga cgcgcgtttt tgcgcaggtc
     8521 gatcccggag acggtgcgta ccgtttcggc cacccggtgg aacgcacgtc ggtagcgtac
     8581 gctctgattc ccgaagacgg cggaattccc gaggtccgca tttgcgttga gagcgccaac
     8641 tgctggtcgc agggctacca ttatatcacc atcgaatgtg ccgacgggac aatcgaaatc
     8701 aacaagccag gatctccgcc agtgatcgcc aaaggatggc ggagtgctga agaagaactg
     8761 aaggagctgc aagaggagcc tgacccttca acgctggcag tgcgggatct ctttgatgct
     8821 gtggagcgcc accgtgagcc cgtttcgaac ggaataaccg ggtacaaagc tgtggaattc
     8881 atcctggcca tttaccagtc ggcattgaag cggtccatag tggaattgcc gcttcccttg
     8941 gacacgccgg acccgttgcg acactttttc aactgggatg gggtgattcg tggcggcagg
     9001 gggacggcat tcagtactac cggggccgtc actaccacag gaaaaatcag ttagggacaa
     9061 tgtccgcgtt ctgctccaca cagacgcgga accacaactc caggttgatc agattccaga
     9121 tgttgaacgc ctttcttctg ctccgtgccg cagcagcgaa tgaggcgatc aatcccggga
     9181 ctttgcgttt ctcgatgaat ggccgttcca ggaaggcctg gctcgtcaga atgtcgcgtg
     9241 cgaaaccgga gagcttctcc gaaaaccagt gttcgtaagg agtgccgaaa acccttttca
     9301 atcgggcatt cacgatctct ccgggaagct ttccctccat gatccggcga agtgcgacct
     9361 ttgttgtcgt tccgtctact ctgagatgtc gtggcaatct gagagcggtc tctacgaggt
     9421 ggtggtcgag aaaaggaacg cgggattcaa tggagaacgc catgcttgca cggtcctcga
     9481 atgtgaggag atacggaagc atagtgacga acatggccgt gcgctgatcg tcttccaatc
     9541 gcgatccagt tgtttcgatt gtggggatat cgaagcgacg gaaccgggag caccattccc
     9601 gcgatagcgg caaaccagcg gcgttccatt tcccctcgat ttcaaaaaga tggtcccgac
     9661 tgaagaatgc acgtcccgcc actttgatca accgccgtgc gccggcggca tagcccgtcc
     9721 ctctccggtc ttcaagcaaa ctgaggacgc gaaacgcatc cagaacccga ccggaacgca
     9781 ccagatcggc cgcagcgctc tcgacgaaaa aattgtaccc gccaagggtc tcatctgatc
     9841 cctgaccatc cagaactacc ttgacgccac gctggtggac gagcctcata acctcccact
     9901 gggtggcggc tatgtacccc gcatgcggtt cgtcgtgatg gtagaccact ttcggcagtt
     9961 cgtgcaccca ggtgtccaat tcaggggaaa cgaaatgagg ctcagttttt ccgttcatca
    10021 acctggtgaa tgcgctcacc ggttcgcgtt cgtcgtacaa cgggtgtggc agaaacgcag
    10081 tgaacgtttt ggtgggtatg gaagagaact cagtcgcaat tcgtgtgatg gaggtggaat
    10141 ccagaccgcc ggaaaggcac acaccgactg gaacgtcact ccgcatctgc aggcggacgg
    10201 agtcttctag cgttgcttcg tattcctcga gccattcggc gtcgcttcgc tgttctccgg
    10261 attgtattgt gggaatatcc cagtaccgtc gtagcacgct cccgccgtcg cggtgaacgg
    10321 tcagcgtatg tgcgggcggc agtttctgga tggaggaaaa gaacgtgttg ttgtcgacgt
    10381 tcagcagatt cagcaccaga taccggtaca cgaccctcca gtcgggcccc cgcggaacga
    10441 tgcctgacgc aagcaccccc ttcggttcgg acgcaaacag aaaggtcggt ccgatttcgg
    10501 cgaagtgaaa gggttttacg ccaaagcgat cccgggcgca gaaaagtgtc tggcggcgag
    10561 aatcccagat ggcgaacgag aaaattccgt tgaaacgttc tacacaggac tctccccact
    10621 gctcgtatgc cgcgatcaga acctcggtgt cactcgttga tctgaagcga gcgccccgcc
    10681 cctccagctc agcgcgcaac tcgacgtaat tgtacacttc tccgttgtag gttatccaca
    10741 gagtgccgtc ccggttgctc atgggttggt ggccggtttc ggtaagatcg atgatggcaa
    10801 ggcggcgatg tccgagcgca agatcacccg agacgtacgt gcccctgtcg tcggggccgc
    10861 gatgtgactg gacgtcggtc atcgcgctta tcatctgagg cgaaaccggg cgcccatccc
    10921 tatgccagat tcccgcaata ccacacattg gcagctcgat tcggcggagt cgttggcctc
    10981 ggcttcaaga gcgcggccga gaaccgaagt gtgggataaa tataccaacc acgtgagttc
    11041 gttccggcgt gggagtgtcc cacaggctgc agctcggtgc agcgctgccc ctctctcccg
    11101 cgtcgctgct cccgtctgca gtcacagcga acttcgcgcc gggaatcctg tcttgcaaaa
    11161 gcgtatcgca ttccttacct ttttctgctc acgggagaat gccccgtgtg tcgtcggccg
    11221 gcggttttcc ggcatccagt caaccttttt ccctgccaca tgtgcgggag gaggatcagc
    11281 aatgcgcaaa tggttctggt cgggaattgt tgcgattact gcggcggccg ccaacgtcgg
    11341 cgcagcaggc ttcgctcttt acgagcacgg agccgctgca acggcgaccg cgggagcgtt
    11401 catcgctcgc gcaaacgatc cgagtgcgat ttactacaat ccggcgggca tttccggtca
    11461 acgcgccggc gtgatgctgg gtacgacact tattacaccg agcggagatt tcgcaggcgc
    11521 aaatccattt cctggtacgg gctacaccgc caagcaaaaa gatgccctct tctttccttc
    11581 gcacctctat gttgcatccc ccttgggtcc cgtaacggtc gggttgggcg tgtttaaccc
    11641 gttcggactt ggaaccaact ggccggagga ttggaaaggc cgggacatct cgacaaaaac
    11701 ggagatccag acgtactact tcaatccggt ggtcgcgtat tcggtgggtc ccgtctccat
    11761 ggcagcaggg atgcaggcag tgtatgggtc cctcgaactg aatcggaaag tgaagcaccc
    11821 gctcctcggc gatgatgttg cggctatgca tcttgaaggc acgagcgaca tcaactatgg
    11881 cttcaacgcc ggcgttcaat tccgcccgat gccgatgctc accgtcggtg catcgtacag
    11941 aagtcaggtg gattcgaagg ttaacggaac ggctgcgttt gacagtatga acgcgaaatg
    12001 gacgatggta aaaaccagcc tgcccgcgaa ggctgatgtc agcacgacga tgccgtaccc
    12061 ggccgtcatg ggtatcggtg tcgcggtcac tccgattccg gaccttacga ttgaggcaaa
    12121 cgttgtccag acgcagtgga gcacgtttga cacgcttact atcgcgtttg cggcgcctta
    12181 cagtgcgctg acagagaaaa ctcccgagct gtactccaat gcgcgaagct atcgaatcgg
    12241 cgcggaatac agattgcctg cgtggtcgct ggcgttgcgt tgcgggtacc tgtttgacga
    12301 atctccggca cccccgatgg cagttacccc gcttctgccg gacgctgatc gtgactcctt
    12361 ccaggtgggt gcagggtaca caatggggaa gacgacgttc gacttcgcgt acatgtatct
    12421 gaaattcaag gaacgttcga cggaaggtct gaaccccaag aacttcaacg gaacgtacaa
    12481 taccactgcg aatttgttcg gtttctccgt aacgcaccga ttctgatcga aacttgatcg
    12541 ggggacgccc tttcaggcaa tgaagtgact ggaagggctg aatgagaggc catggaaacg
    12601 tggccggagg cgcgggggat acgtcctccg cgctttctgt ttccgggcct gagatttgtc
    12661 tgatgacagg ttacgattgc tcgaggcgga ggaagaggaa cggaaactct tggatcagag
    12721 aatgagcaga gtagaggggc cgccgggatg aaacgcttca gatcagggaa aaactgttga
    12781 aggctttttg atgctgccct gcagagatat agtatcacca ccccgtaccg aggcggtgag
    12841 tccgcaccgc aggggagccc ccttcggtag acgtgtgttg aaggttcaat aaaacggcaa
    12901 cccctccacc catggaatat gattgccctg ctcgtgtctg tcaatacccg ccgcaaaacg
    12961 tgatggagtt ccctataaac gggcgttggc ggcagcccgc atcacttcca caggaggcat
    13021 catccccgtc cacaactcaa aagcgcgcgc gccctggtgc accagcatat cgagtccgtt
    13081 gaaagcaact cccccggcgg cgacaacctg ttccatcagc cgtgtggttg cggggctgta
    13141 atttgagtcg atgcacacga tgcgcggtgg aatgcagttc cacgcgtcag gcagagggct
    13201 gatgttcgcc agcttaccgc gcccgaggtt tgttgcgtta atcagcaagc cgcaaccatc
    13261 aagagcgtgc cgcgccgcgt gaggttcgag gccgaacgca gttaccgagg aggttgctcc
    13321 aaattcgcgc gccaattcct ctgcgttcgc cagggttcga ttgagaatcc gtatgctctt
    13381 cacttccggg agagtagtaa gcgcgtacac aatccctcgc gccgccccac cggctcccag
    13441 aacaacaaca acgggcggaa cggtgtccat tttcgctccc acccggatgg cctgaaggat
    13501 tccgtacaca tcggtgttgt cgccgagaag ttgtccgtct ttgttgacga ttgtattgac
    13561 cgctcccact gcttttgcag cgggtaacaa ttcgtccagg aattggatga cagccttttt
    13621 gtgggggact gtgatgttca tccctggcag acacagacca cggacggcat ttactgctgc
    13681 ttccacgcct tccggaacaa catgccacgc aacatacacc cagttcaatc cgagcgcttt
    13741 gaacgcggcg ttatgcatcg ctggcgaaag gctgtggctt accgggtcgc caattacgcc
    13801 aacaacgcgt gtggttccgt cgatatccac ctctgatcgc ggctccctgt ccgtggtgtc
    13861 tggctatacc tgaaccaaca ctgcgaggac aagcagtgtc caggaaatga ttcgtttgaa
    13921 cctttccttg ttgattctgt tgaaaaggtg cacgccgatc aatgatccgg caatgatcgc
    13981 cggaacgagc cctgcgatgc acgccagcat gcgagcgtca atcgctccgt tcgcgatcaa
    14041 caaggcgctc tttgttcccg aactcacgaa gaaggtggtc agaagcgtcg ctttgaacgt
    14101 ttcggcatcc catggttggc gagaggcata tgcaaccagt ggagggccgc ctatgttcgt
    14161 tgcgcccccg attaccccga caccgagacc cgccaccgcg gcgacccagc ccggcagagc
    14221 gtgccttcgc accttgaacc caataaggct ggtcacgccc acatacacaa gcagggcact
    14281 gaccagaaac cggatcagga tcaggttgcc gcccagaagt atcgcgagac cgaacggaat
    14341 cccaacgagc gtaccgccga cgactggcag aacaagcttc caccggatgc ttctgcgcac
    14401 gctccaaagc gtggtggata cgttcacaaa cgccaagggg gaaacgagaa tggaagcagt
    14461 tcgcacatct attacgagcg cagccagtgc catggcgacc ataccgaagg caaaacccag
    14521 cgtaccctgg accactgcag caccgaatgc tatcaacaac agtatgaaga aatcctggct
    14581 tccaaaaaac acgtactctc ctaaccctcg gaattgccgg catcaattct gagcacaacc
    14641 gccgggtcga tctcatccgc acagatttcg aatgtttcac ctgattccaa tgcgaacaga
    14701 tcgaaccttg cggccggata ttcggtttca tcaacccgta caacgcaact tccctgggcc
    14761 accaggagga gaactccgtt cgatgcgcgt tgcgcctcgg tggcctcccc gcgagcgaag
    14821 aacctgacgc tcgcaccgaa cgacccgtag gacgcaggga gattggccag gagctccgcc
    14881 tggtcatttc catcggggag gtcgtacaaa gaaacgtgcc gcactgtcca ctccctaaga
    14941 aaaaagtctt tgtccgtatt attgggatat tcagctatcc catctcctcg atgagcacat
    15001 gacgccgctc ctcggcggaa cgataaactg ctttgacgat ggcaacgtca cgacgtccaa
    15061 tttcgccagg cacagggacc ggagcgtcct caagaatcgc acggacgaag gcggcatgct
    15121 ggtcgtattt cccggcgaat ctggcctgcc gtggcccgta atctgcgggg tcgagcaccg
    15181 tcatctcttt gccacccctg ggggtgtact tgatgatatc gccgtcccag ggaagctctg
    15241 accacaggaa tgtctgtccc gccgtcatca ccagagatcc ctttgttccg taaagggcaa
    15301 aaagccctcc ctcagccgcg taccccgcct cgaacgaacc gtacacacct ccgcggaatt
    15361 ccgcaacagc atgggtcaga tcatccccac cgatcgaatg tttgcacaag cggttcgtgg
    15421 aaagtgcggc aacctgaacg atgtcgtcat ccagaagcca ctggaaaaga ttaatggagt
    15481 ggactgcaag ctggataaac acgccgccgc cggtcttctc cgcagagcct cgccacgcgg
    15541 tattgtcttt cgcctcatat ccgccccgat gggctccgcg actgctgaca gaggaaatga
    15601 tcccgaagaa cccttgtcgg atcatctcct tgatctcgtg gaagaggggg gcacccagag
    15661 aactcatgta aaggcccagt ttgcgattgt tgcgctgggc cgcagcgatc atttcatcgc
    15721 actcacggat cgtgggtgcc atgggtttct gacaaatcac gtgctttccg gcctcgtggg
    15781 cggcgattgt ctgttgcgcg tggaggtgat tgggggtgct gacgtccaca aggtcgatgt
    15841 cggggccaag caagtcctca aacgtcgttg tccagtttac gttgagtcgt tctccctgtt
    15901 cacgtgcctt cgcgggatcg atatcctgta ccaacacaag ttcggaatcg gggcaggtct
    15961 tgtacgcctc catgtaccaa cgggcttcgc caccagcgcc cgtgacagcg acgcgaatct
    16021 tgcgtgacat aagattctcc gtaatgggaa gatggtatac tgataatgtg gattatgtca
    16081 tgccgatcaa gccagcttgt cgtggtcagt cgtaatgtcc cttctttgaa gagacctcgg
    16141 ctcccacaac cagcgatttt gccccggtct ggagtctgcc gacccggtgc cccggcgcgt
    16201 gtgacgactt ccctttgaac tgcggcaacc acggagcctg cgcttcgagc atctcatcga
    16261 ccatgttcct gatttcgtgg ggggcaagca ccgcggcggt aagaggatca agcatgcacg
    16321 cgtggtagac ggcttccggg tcgccttcga tggctgcctt cacagcaagc tcctgcgcgg
    16381 aaatgttcga ctggcaaagc gccgcgcact gctccgggat atgaccgacg tacgacgcgt
    16441 gcagcccaag cctgtccaca aacacgggca cttcgacaca gcaatcgtaa ggcagattgg
    16501 tgatgaagcc tttgttgagc acgttgcccg cgaaccttgt aatctccccg gtaagcttcg
    16561 cgttcatgat gtttgaagcg tactcgaccg acttgtaccc gcggcggaac ggaacgggtt
    16621 ttctcccgtc cgccaactca cgcatctctt ccagatattt gtcacggtgt tgcaggcagc
    16681 tgttcaggta ataggctgtt tcccctgaga aacccgggcc gttgaacaat tcgagcaggt
    16741 ccttccgttt tcggaagtag gggaggtatt ccgaaagatg tcctgacgac tctgtcatga
    16801 agtaccccgt tgccttcatc atttcgaacc ggtactcctc gccccggatg gggccttcag
    16861 cggcgagttt gtcccacatt ctgggataaa ggtcgcttcc attgtggcgg aacttaagga
    16921 accaggccat gtgattgatt ccggcagtca ggacgtcgca ctcctctact gccactccca
    16981 gccactggca gagcatattg gtcgtgtgct gaaccccatg gcaaagaccg accgacgtcg
    17041 tgtcggggta catcttctgc atcgcccacg acacaatcgc catgggattg gcgtagttca
    17101 tgaagatgat ttcagggtgc gatacatcct gcatgtcacg aatcatgtcc agaagagcgg
    17161 gtacatggcg cagcccacga aatatcccgc ctgggttcac cgtatcgccc acgcagacgt
    17221 caatcccgta tttgagggga atgcttacgt cgagcttgta cggttccaga ccacctacct
    17281 ggatcatcga gatcgcgaaa tcggctccgg tcaatgcttc tcttctgtcc gtagtcgccg
    17341 aaaacgtgca cgcgagcgta tgctcctctt tcatccggag catggatcgg tacacgagat
    17401 ccaaacgttc tggatccacg tccatcagtg cgaaatggat gtccttcatg ctttcgaagc
    17461 tcagtatgtc ctcgaccagc atcctgcaga accctgcact gcccgcaccc atcattgcga
    17521 ctttggtcac ctggtcgccc tttccatcgt gaagaaaatc gtcctgaact gcgacgccat
    17581 taccaccgcg tcagttgttt gaaacgaggc cacgcgtcat cagcgtagaa tcggtgtccc
    17641 ccatttccca ccacgagccg acatttgtct ttgtgcccgg ctgcggaata gatatcgcga
    17701 atgcgccgaa aagtgctccg aacccctttg atcgggaaga tttcgtcctc cttccctgcg
    17761 accaccacca atggcctcgg ggctatcaag cacgcgagat cgcccatttc aaaccacttc
    17821 aaaatccccg gaacatagtt gtcttcgcag tggttgatcc gaccaattga atcgcggaat
    17881 gtgcacacgt agcacgaggg catggccgcc tttatccgtg gatcgcaaca ggcggcaaaa
    17941 tacgtgatgg ttccgccgcc cgaattgccc atgcaggcga tcttgtcgct gtctagctcg
    18001 gggaaggttg ccaacgcgtc gattgcgcga gagacatccc acacccgctc gccgatcatc
    18061 gtccttccga gcaacagaga taccattgtg gcatggtgac accgggagcc ctcatgtttc
    18121 gcatctttcc gttccccgaa acagcgctgt tccatcacca gggcggcgaa tccttccttg
    18181 acggctctaa tggcgaaatc gcggtccccg ccgctgatag acttctgatc cccctcgtac
    18241 ttcggacgcg cgagacttat gtgcatgccc gtagagtgcc cttgcagaca aatcatgacg
    18301 gggaatggtg cagagccgtg tgtgggaagc aacaggtggc aaggaacgtc ggcgtttttc
    18361 tccgccgtga acacgaaccg ccgctcgacg aattcctcgt gttgcgtctc gaattcatgc
    18421 cggatgttga gggggacctt tgccggcgtg tttcccagaa gttgccgcag cttcgcgccc
    18481 accttgcgcc gccacgcgtc gaaggattcg gcgtccgaat gcttgtgcgg gtcgaacgtg
    18541 agcgcgagcg gggccctgtc catcaaatac tcgtggcaca actcaggggc aaaagagtac
    18601 gtgtccattg ggatttcccc tatccaatca gggctttccg aaatctcgaa ccgcttcgac
    18661 aatcacggga aagcttttct gccagacgcc aggcgggacg ttctcggaaa tctgtatctg
    18721 catgcggccg gtgtgtcctg cctgccgaag catggccatg gcgtgggagt agatctgctc
    18781 cggttcccag agatgcacgg acgacnnnnn nnnnnggaag ttcacgaaaa ggcgcatatc
    18841 cggccaacgg gttgcagcgt cagcgcacga agtgtcgtta tcgggtggcg gggaaaggga
    18901 gtctattccc cggaccccgc tccctcctat ggcctcccag agcggtttga gatctccgtc
    18961 catgtgcacg aacaccggcg cgtcgtcgtc tctgagcatg cctgccagct cgtcatagaa
    19021 cggcacacaa tacccgcgga aataccgttc acctatcgcc ggtgcggtga tattgtcagg
    19081 aaagtcaacg aagggaactc cgtgctcgcg cgtcgcccgg caaacggact cgaatatccg
    19141 ccgctcgatg ttcccaagaa cttcaagaca ctcccgcaga accggcaggt catcttcaag
    19201 atcaacggcg agctgctcga gggataccca ctgaatccac atctgctggt aaggcgtgcg
    19261 gtgaaccgcc accatgggaa cgccgtcgtc tcccagcgca tccctgtcga gcagaaatgg
    19321 tgtcgcatca tcaacgacga cgacgttgcg caggtaggaa agcaggatct cgtagtcggc
    19381 ggaatcgcac acgtagtgct tgcgcgtggc gccggactga tatgcgggtt cgaacacccg
    19441 ctcctgaaca agtgatcccc gcggtgtaat caggcgagca cgttcgccgg ttctcccgtt
    19501 gatctcgaca ggttcgtgcg taataacgca atcaggtgct tcgatccggt atgcggagga
    19561 ccaacggatg atgcccatgt tcgcccgacc gacaacatcc cagacaacct cgttcggcgc
    19621 ggcaagaccg tcgtattgaa cgaaaggcac ccggtcgtgc gggttaccgc gaattactgc
    19681 cagaattcgc tcacgcatgg tcatcggagg gaacaaccga agagaggagt ggtgaacacg
    19741 catcgttgac caaatatagc cggcagggtt ggtcctctcc caccccgctc ggttcctttt
    19801 tgggagtcaa aacgtcacga actcgggccg ctgaaggagc actttcgcat gcgcagtatg
    19861 accggttgca tacaaacccg cctggtacca agtattttgt gtgttgtcat cgtcatgagc
    19921 gccaacccag cgagtgcgac tatgaacctg aatggatcat tcgaggatgg agcggaatcc
    19981 ccttccggct ggacccggca ccccggtggc ttccttggag aggatggaca taccggtgcc
    20041 aggagcattg ggggaagggc tcggacaaag gccaccgtgt ggacgtcaga cgtcatggag
    20101 gtggaggctg gacagagctg gaggatcagc ggatggtttc gtgtgaagaa ggggctggtc
    20161 ttcctccgta ccgagtttct caattccgcc ggtgccgtgc tcggatccgc ggatacccct
    20221 gagatcgcga aagaccgaca cgcatccgga tggcggtaca ccgcggtcga aaccgatgcg
    20281 ccggcttcgg ccgaacacgt ccgcatgagt ctcatcgttc gcggagaagc gctctgtgat
    20341 gacatcgtga tgacgccgct catcgccaat ctgctgtaca acccaacgtt cgaagccggc
    20401 agcaggggac gcgtgacctt ctgggatccg gaccccgtcc ttacgagggc tactgcgaaa
    20461 accggttcag gcatcgccga tgaaaccggt gggcggaacg gcgcggggct caggattcgg
    20521 tcggattcct catggtgggc gatgcggaat attcagcaac cgattccggt tggcctgaca
    20581 acgttccggc tctcgggatg ggcgcgctgg gaaggagaag cccccgatat cgccatcgcc
    20641 tggacggata acggtggcgt tttgcacggc ctgagccccg caactccgcg cgaaaccgtg
    20701 aaccgatgga cccgttttga ggtgagcgac gctcatcccc cggttcaggc ggaaacgatg
    20761 acggtttcgc ttgttggaaa catcgggact gcgtggttcg atgacttcca actggaaccg
    20821 gttgcgccgg ccacaaacct tcgccgcgtg gtcaaagttc atgtgaacca ggtcgggtac
    20881 gaacgcaacc tgccgaaaag ctgcgttgtc gcgacgaact tcgttccgca cggggggagg
    20941 aatttcattg aagtgcatgg agaggacggc cacacgctcg gaagttttcc tcttgtcggt
    21001 cctgagcgaa tacacaacgg gatgccggac gactggggcg aatacttctg gcgcgcggac
    21061 ttctcgtctc tgaaagagac cgggcgctgc accattacgg ctgtggttgg cggcgtgcga
    21121 ggtgagtcgt acccgttcct gatcggcgac ggcattctct tcggcgggac ggctccactc
    21181 ggcgtcgagt ttttcttcgt tcaaaggtgc ggttttgcgg ttcccggctg gcatgcggcc
    21241 tgccacctcg atgacgcgct ccttcccgac ggcacgcata ttgacgccgt tggaggatgg
    21301 cacagcgccg gcgattacaa caaactcatg tacgagaacg gtgacggcgg cgtgctctat
    21361 gcccttaccg aagcgtttcg gacgaaacag caggttttcg aagcctccga tagagacaac
    21421 gacggggtgc cggatattct cgatgaggcg cgctggggtg cggatttcgt cgtgaaaatg
    21481 caagtcccgg aaaccggcgg tcttcggaag gatgtatccc agggaccggg ccgaacgtgg
    21541 atgaagtggt gtccacctga ggttcacact gataacatca taggtacgga tgacgacccg
    21601 gtcattttgc ccggggaggg gaactccccg cttgcgatcg gcggctggat gcggcttgca
    21661 ggggagttgg aacgacgcgg agaggatggg tcaaagtacg ggtcgagcgc ggagcggtta
    21721 tgggggtatt cgacgcagaa cggggaaacg gggttttcgc cgctgaatct gctcagtgcc
    21781 ctcgatctgc atgcggcgac aggcgaacga cggtacgctg aatttgcgtc ccggtgcgtg
    21841 gataccctcc ttgcgagcca ggtcccctcc gggagacacg cgggtgcgtt cggggcattt
    21901 ggcgagcacg cggccggggc actgggcacc tatgcgctca agttcccgga ggattcccga
    21961 attccccgca tacgggctgc tctggcaaga tacatggagt tctgcctcac gacggcggag
    22021 aaccctttcg ggctcgcgaa gcaatccgtt ggcgaacagg atttcttctt cgagccgaca
    22081 tcaactctcg gccacaattt cgaaattctt gcccgcgcat ggtccgcgat aatagcatac
    22141 caggtcactg gacatgaggc ggcactgacg ttcgcgctcg accagatcga ctgggtgatg
    22201 ggcaagaacc cctacgggct ctgtatgatg gaaggtgccg gaagtaacaa ccctccacgg
    22261 taccaccacc ggtacgattc cattccgggc cgtgagcgcg gtgcagttcc gggtgcgata
    22321 ccgaatggct tcgtccgcac ccccagagcg cttgaccagc ccggtttcga tatgtcgcga
    22381 tcccgaaccg aacgtgacca cccgtcttac cgaaccagcg agccgtggtt ggtgcacaac
    22441 atgtggcatc tgttggcagt ttccgcgctg cagcgagtcg ttgatgagcg tgcgcgttag
    22501 ccagaatacc cggggacctg tgtctccacc cttgctcacg ctatatttga aacgttttcc
    22561 cgtcatcctc ccgttgatgc aggcatcggg gtacgacccg gaatcgcctt cctcagtggc
    22621 cggcaggacg cttctgtcca ctcacatctg agaggattca aagttccatg gataacatct
    22681 ttcggaacaa aatggtccaa gggattggac tgggcatcgc aatctgcata gtcgttgtcg
    22741 gtgccttttt cggtatcaag tccttgatgg gaccaggctc cctgaaggag cagctcaccg
    22801 gccagaagaa gtatcaaccg ccttcgagcg caagcacgga ttcagccttc gcgttcccga
    22861 cgattttcat gcgtgtggta ggcgattctg ttcgggggct ggtaagcatc gacccaacga
    22921 agaaaatcga cgcgggaacg gaacttggcg tgcaggaaat ggtgaatcgg ctccgtgtgg
    22981 ggcaccagac acttgcgcag aatcccggac cccgcggggt gcccaatgcg ttcaatctcg
    23041 ttcttgataa gagctcaacg tacgggcaag cgatgctggg catctacata ggcgtaaacg
    23101 gaactttcca gatgccgttc ctcataacgg ccgagggtaa ccagcactcc gtagagcctc
    23161 cgcggatgca tccgcagttt ggcccgatgg gatgggccgc ctggatctgg tggctgcctg
    23221 atcagaggat caaatgggtc gtgtggccgg attcgctgga aggtaacttc acattccacg
    23281 ttcttcctgc gaagaacggg aaaccggata tcgagggact ccgcggcaaa ctgagaacag
    23341 caatggacgg gaaggcgcac atccctccgc cacaacagcg cctgatcggg ttctgggggt
    23401 acgacagcct gccgtacgag agtgctatcc agttcttcga cgtgctgcga tatccgaaaa
    23461 cgggtgaacc gctgtgcagc agactcacgt tgatggaagg tgagatgatg tcgcctccgg
    23521 ccgacagcgt ctgcgtggaa taaggtggaa ggcggggggt acgccgtgga gcgcgtcgta
    23581 ccccctcacg catttggccg gaagcaagta aggcaaggtc gaaggttttg acgacgataa
    23641 ccgtgggttt gcttggagcc gggcgtggtc tcagcctggc acagcttttt gaccgtgtgc
    23701 ccgggtaccg ggtggtcgcg gtctgcgacg cgattcccga acggcttgac cgtgcccgtt
    23761 ccgcatgccc tcgcgcggaa ggtttccgtg actacgcggc gatgttgcgg gagccctttg
    23821 acgtaatgat cgtcgcaagc gctcctccca gtcatgcgga tcacgtatgt gccgcccttg
    23881 aagccgggaa ggcggtcctc tcggaagttc ctgctgcaca cacgctcgat gatgcccaga
    23941 ggatagtgga taccgttgcg aggacgagcg gatattacat gctcggtgag aactgcaact
    24001 act
//
LOCUS       DAOTNG010000006        23808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2203_length_23808_cov_5.260009, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000006 DAOTNG010000000
VERSION     DAOTNG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 23808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23808
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_23808_cov_5.260009"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="P5179_00645"
     CDS             complement(<1..806)
                     /locus_tag="P5179_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93760.1"
                     /translation="MLIRLVCRTGVFSFFLLCTTVTASPQNPDGDLSNRLEVQQAELA
                     RIEQELAATNAEMRMAKARERALASQVVAASNRAGLTKHAINRLTEAETALVSDMNSA
                     QIRTQQTREKIERRRESISLRVRTLYIHGRREPFQRALMASSLAHWLAAREYLTAAAR
                     RDANEIRSLKRDQVRLDSLTALYSRQKMLLDTLITTRERQREELAGAEMEARRLLRAM
                     RAKRRTLEQSAEALESQRAESRQRIAEFLSSQTGASPTRIPLLEQGPGLH"
     gene            complement(800..1708)
                     /locus_tag="P5179_00650"
     CDS             complement(800..1708)
                     /locus_tag="P5179_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRS93761.1"
                     /translation="MFWSTSTTEAWRAVSSAKRATISSVLIMAVSLAILGILGLGALA
                     FHNEAQTAKRWITAEVFLKDGLEPEAIQLVRRRIVAIEGVSNARLITKAEALVRFKRF
                     FGSELVDVLETNPLPQSYLLTLSDEGRTPEGLKAIARKAGSFPEVESVDADVEWLTIL
                     ERISFTVNVVLLLFLGIVGFAISVVISRTIGLGIASRAEVVTLQRLLGASEWFVRRPF
                     VILGVAQGAVGGILAALIVLACSRFAYAIPLVGHSFGGTNAHVAAWSLVGIGVVLGLA
                     GSISTLRSSLPRDPWESEQVTRNSLC"
     gene            complement(1866..3392)
                     /locus_tag="P5179_00655"
     CDS             complement(1866..3392)
                     /locus_tag="P5179_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS93762.1"
                     /translation="MKSRAVAECVFVAILNACAFEAIPASQEQYVRLSLQECRIRALQ
                     NNLSFRVKRLEPLISEARLKAALAPFDPAFTASFTGSRTQQPNSLVFDPSTGTYISQS
                     VPAQNSLGTRLGISKAFSFGTNASINLNNSWSKGSGSSNYGSNVSLSVSQPLLRGFGR
                     DANEFGIRIARANRETSMKQIVADASAAVQKVESAYWDLVSARENLRVAQMSHQYAQQ
                     LLERSRARAETGAQALRDVIQAEAGVATAYQGIISAEARVRDAEDTLRRLTNIAAMED
                     GWSFGIVPTDTTHIDSGTPSFDSLFAAALENNPAVFQAAQAVETSKFTLKYRYNGLLP
                     TLDASTEVVLRDGDRTAARSMDDLPTAAYPSWTLGLRFSVPLGNRQSEASYREAIIAV
                     RTAELELQDARQAVQADVRSALRRVETSRQQIAAAQETKRLRESTLENEQERLRLGLS
                     TNYDVLQVERDVADARRAYVEARVEHEKALLALDVATGLLLRRRNIGVEMDGSELTSR
                     "
     gene            3691..4440
                     /locus_tag="P5179_00660"
     CDS             3691..4440
                     /locus_tag="P5179_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93763.1"
                     /translation="MLKITKKQRERWPRELILSEKSEELEEHLTLLNAEDRAAVERVS
                     REVDSKIVEAARESVKELKMLEEGRCPACSQKTRRFLFSTICEHCGWSGYSRPLRAKV
                     VIHLRDGSALICEDVYHTPDELMCVTQDVVRYRVPRNVVAYVEFDHAEEDIRQRREQL
                     AREEQGQCSWCMKPVVRTNEETRVTFAAFAIYQERFLFCSIHCQTAFQKQYPTRIHRD
                     CYHRDCSKCEECQKRFDDTSYETFLEEEFVH"
     gene            complement(4475..5443)
                     /locus_tag="P5179_00665"
     CDS             complement(4475..5443)
                     /locus_tag="P5179_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRS93764.1"
                     /translation="MGNQPDRHHLHAPLEIGGTVFHAAEIAQMPTFLSYAGNARPWLW
                     AAYFPFLLAYSDSPARAVMLGEHGGSLFVLLHRAVRGHGHIDFLLPPLGSGYEGALEN
                     LTGSLARLNGPIETRFLWADLPGAAYGSRRGWVVTPYEHEYVYQRRALIELRGNEFRA
                     LRKRIHRCEREVQPEFRAFVESDVPECEALLREWQDMHRQDPVPVFDFGYTLSAIRSA
                     ARFRPPHLVGVVAVVGGKVRAFAFGGMIREGLGQFFVLKSDPACKGLAEATRLVLAER
                     MEGCDLVNDAGDLSKPGLAQHKRLFRPVSFVPTFKMACRSALSPDA"
     gene            complement(5455..5811)
                     /gene="speD"
                     /locus_tag="P5179_00670"
     CDS             complement(5455..5811)
                     /gene="speD"
                     /locus_tag="P5179_00670"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRS93765.1"
                     /translation="MTCSPPVHLLIDFVVDDPVLLNTPDPSMSFLRESVLAAGATILS
                     EHTHQFSPVGFSGILILAQSHASIHTWAECRLVSIDIFACGAIDTEAAVDVLRNRFRP
                     SSERIEICRRGVGAFK"
     gene            complement(5808..6992)
                     /locus_tag="P5179_00675"
     CDS             complement(5808..6992)
                     /locus_tag="P5179_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HRS93766.1"
                     /translation="MALREGHEQLHAGDGGQILSEVASASHLREGREGVRTILRAIAT
                     LEPVPVRVLASKVRLPLPVTVAVIGELSARGVTERAPNGVRLTSAGASCVETICGDAG
                     HDETTGLSAVLDDLAALRPQADVTLDQAKATVGTLVRRVNYLLKNDSLWGRRILFLGD
                     DDFVSVAVARWVHQCAGGEPAGPTPTLTVVDIDGRVCDGIKKAAPSVQTFVHDLRKPL
                     PDSLQHRFDVVFTDPPYTPGGAELFLCRAAEAVEHEGAHCFFSMGHLDPGGMRQVQAA
                     ISGIGWVIVEWIPAFNEYEGSSVLANVSLMAHLIATPEIAPTLRETYEGPLYTADVRP
                     VIRVYRCLGCSAEWEVGQGKRWITVERLKEDRCPLCRSDRFLRARQYSRAEFEKPGGA
                     QN"
     gene            complement(6970..7800)
                     /locus_tag="P5179_00680"
     CDS             complement(6970..7800)
                     /locus_tag="P5179_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93767.1"
                     /translation="MGCTIFWEAQEFSLSRQAQVRDELVALAQVAGWEFEIAPDELTG
                     AFINLDLQPGEARIIERRLRLCGVTLFPFGHDEQGIRPRLSFVFDNSNLSNPNVRNRL
                     ITIAPPSYWTYWWPRLREQYPLLMNLPDKPILCIRGDGNMRVRRTELPGFIKFVNTLR
                     AGSLPLLDIRTSDKLARKMISEPQGGQFGARGARRQEQESLADIAGGLAALFASSRFD
                     EWRAALQTEDDIEREQARANRAAALPQERERRSSRQDRPGTGTRGFSFGSTYGAPGRS
                     "
     gene            8120..>9294
                     /locus_tag="P5179_00685"
     CDS             8120..>9294
                     /locus_tag="P5179_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRS93768.1"
                     /translation="MVSDADLLDKITDYTRKQGGDTAIERLLANVVSQVREFAEARAS
                     EIKRLSDIGIALSAERNLERLLERIVDEARRFTRADAGTLYTVQINPATNLPELKFDI
                     MQNDTMNTRQGGTSGNPITLPPVPLVKDGKPNHNNVSAYVADTGETVNIPDVYIAEGF
                     DFTGPRKFDQITGYRTKSMLVAPMRNHEGEIIGVLQLLNAKVPGGERVIPFSDEYVDL
                     IKSLASQAAVAITTARLIQDLQNLFESVIRLVASAIDEKSAYTGGHITRVAGLSMKIA
                     EAINETTEGDFADVKFTPDELNELRIAAWLHDIGKITTPEYVVDKGTKLETIFDRIEL
                     VKTRFEVIRRGVIADAMEYKMRLIANGADPEALKAVDEQLAVRLEEIESDKEFVARC"
     assembly_gap    9295..9394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9395..10002
                     /locus_tag="P5179_00690"
     CDS             <9395..10002
                     /locus_tag="P5179_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRS93769.1"
                     /translation="AVRLEEIESDKEFVARCNTPGEFLTDDAIDRLKAIAAKTFLLDG
                     VETPYLTENELKNLSIRKGSLTEEERQIINNHVSVTIKMLSQLPFPKKLSHVVEYAGA
                     HHEKLNGAGYPNGWTAERLAIQSRILCLADICEALTAADRPYKPSMPKERAFQILGFM
                     AKDQEIDERLLKLFLEKEVYDRYMVEYKAQKEAEAAAATAK"
     gene            10131..11024
                     /locus_tag="P5179_00695"
     CDS             10131..11024
                     /locus_tag="P5179_00695"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS93770.1"
                     /translation="MSKPRLGFIGLGIMGRPMVRNLLKAGYQVTVWNRSEPGIAACVK
                     EGAQKAASPADVTSRCDIVLSCVTDSPDVRQVYLGPNGVIDTIRPGMIAIDMSTISPA
                     VAREVASALEAKGAKMLDAPVSGGEKGAIEATLSIMVGGDAETVATATPILQALGKRI
                     IHIGPNGMGQTVKLCNQIVGALNDLAMCEGLILAAKSGADVSKMVDAVSAGAAGSWAM
                     SNLAPKILKRDFSPGFMVKLQQKDLRLVLDTAKELQIALPGTALVNQLFQSLEGKGCG
                     DEGIQALVKALEQMDGAEVRG"
     gene            complement(11095..12231)
                     /gene="bshA"
                     /locus_tag="P5179_00700"
     CDS             complement(11095..12231)
                     /gene="bshA"
                     /locus_tag="P5179_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRS93771.1"
                     /translation="MRIGITCHHTYGGSGTVATELGMALAKRGHQVHFVTFAEPYRLQ
                     FDPNIYLHQVDVMHYPLFDHPPTTLSLAAKMAETICTEKLDLLHVHYAIPHATAAYLA
                     LKLCPDHNVRLVTTLHGTDITLVGQDPSYFSITRFSIEQSDVVTAVSQWLKDQTLSRF
                     KVAHPIEVIYNSVDTERFRRVSVPACQRARYAPGGEPILVHISNFRPLKRVTDVIRAF
                     ALVRKRMAAKLLMIGDGPHRMPAQEVALELGVMGDVWFLGKQDRVELFLSLADLFLLP
                     SEHESFGLAALEAMACEVPVIASRAGGLVEVIEDGVSGHLVEVGDTEMMAELATRVLK
                     DQELAARIRAAALDRVHRLFSADKIVAEYEQLYRTALSSPKHQS"
     gene            complement(12256..14178)
                     /locus_tag="P5179_00705"
     CDS             complement(12256..14178)
                     /locus_tag="P5179_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93772.1"
                     /translation="MPSSGLIEKTYRQTLNQAREIVSERLSALVRATGGDCEDATEDI
                     AQSIVGTEQVVRLILSTPYDRSLYRPVLLEAADEVGRTFFPEISKRFFSPLSDAPAVE
                     SNAAEPERSLDSPQLEQAALALPPHLRSYFKKLLENAAERLPTARLSAIATDLLAERL
                     ERLQRHFPDGSIPPLSNAPVTDRMSDDEIILHARYVAARLAAAFPWGFFSKDTRRTGI
                     AVNYLVMREMRKSPEEALQEDALSYIALGLGPALRRCGGSVNRLLALGFPERIKPWMG
                     SHVPTGYWEETANRRDAVKWLVEQRLGIPPEAIGRAVHQNTISKKAFREAGLTWLLKN
                     VYGWSVAHALAESYPQLRPWECFCRVPVETWHGDAGRLLSSEAIGWAFREEGMQVATF
                     RTQDAARVIKSTLNRWHLGAAFRIGFSENSFALLNSLFPNTFELWEVTHVPREAWKDP
                     ALRTNAVQWLLKEYHVEVEKIPVAIRNGLLSPDVFKMRGLDGLLRQMGSVWRVIDDVF
                     PGRFARWELGSVPNSYWRARSSVREAAIWALSRLGIELARVEDALRSGTLASRALTEM
                     GLGSLVNTLFRGDVRSLMQVAGVLPSARRVPLHRFYRQLRQDRVASHASEVEDRVQAL
                     RARQKERRRRTEEHWC"
     gene            complement(14211..15266)
                     /locus_tag="P5179_00710"
     CDS             complement(14211..15266)
                     /locus_tag="P5179_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS93773.1"
                     /translation="MRIILRDLSYRYNGEAGVTNLNLQVESGELFGLLGPSGCGKTTT
                     LRLLAGFIQPEHGHVFFGDEDITGKRPEQRNVGMVFQSYALFPHMTVHENVEFGLLAR
                     GVPRKERGRRIRNALELVQLAPMERRSVADLSGGQQQRVAVARALVIEPTVLLLDEPL
                     SNLDARLREETGQELRALQRRLGITTVYVTHDRAEAFALCDRIGIMQHGRLIQVGAPQ
                     DLFASPGSLAVAQFIGHTNILPVEHIQEAGNGWTAQTGPHTLFGTRTLPQEPTAALAV
                     RPHEMTLDTTPISSKNTLLMRVSGRRFLGADMEISVQDGNLRLKILQRFSQSRVPEED
                     SQVYVHIPPEALIPLAR"
     gene            complement(15287..16072)
                     /locus_tag="P5179_00715"
     CDS             complement(15287..16072)
                     /locus_tag="P5179_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRS93774.1"
                     /translation="MTPDDRPAVTGSTEANTGAKGIQRFLTLPTAAATGAFLALLLIF
                     VLGNWVVMPLITRGTDVVVPDVRGMDVAEAKRLVEEQGLVFVNDSSDYATDESIPVDH
                     IVMQSPAPDVSVKRGRSVRVTVSRGAPKYVVPNVRGISLNDARVRLEQARFRVGNVSY
                     VLRTNEDLSEPYVQDQFPDAGTSLQKDAQVRLVVSIAPEMPALVGRDLGEASRTLAAM
                     GIQIGETTSEPDETAAPGTVLKQSIAPGTRVFASDRVDLVVTE"
     gene            complement(16069..17070)
                     /gene="pdxA"
                     /locus_tag="P5179_00720"
     CDS             complement(16069..17070)
                     /gene="pdxA"
                     /locus_tag="P5179_00720"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRS93775.1"
                     /translation="MNVSKPRLAVTMGDPAGVGPELCLAVATDTRATGAADLLFVGTE
                     SILQSVAKTLGRPLPDPAHICSVAEPEVAVPPGAVSAEAGRLSALYLDRAIVLAQKGE
                     VDGIVTAPISKEAWHLAGVPFPGHTEFLAAKTGTKEYGMMLVHGTWRVLHVSTHCSLR
                     DAISRVTQERVLRMLLLFDGVLRDLGIASPRIGVAGLNPHAGEAGLFGDEEREHILPA
                     VLEARSRGVEVLGPIPPDTVFARARLGEYDGVLAMYHDQGHIAVKTLAFEPSKDGGWA
                     SVHGVNVTVGLPIVRTSVDHGVAFDIAGKGIARTESMLDAIQLAARMALVRAGGSVR"
     gene            17342..19159
                     /gene="mnmG"
                     /locus_tag="P5179_00725"
     CDS             17342..19159
                     /gene="mnmG"
                     /locus_tag="P5179_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRS93776.1"
                     /translation="MAAARLGFDVVLMTMDVGKIGEMSCNPAIGGTAKGHVVREIDAL
                     GGVMGKLIDATGIQFKMLNRSKGPAMWSPRAQADRAAYRAAAQALVSSHERVRIVAEQ
                     AGQLRIEGGRIVGVIGVSGTEISCRAAVVATGTFLNGLMHVGLSASIGGRVGEPAASE
                     LPRSLAAGGLALGRLKTGTPPRVDGRTINYSVMTPQYGDEPPPFFSFETPPRSVQQVP
                     CWLTWTNTATHRLILGGLDRSPLYTGRIKGTGPRYCPSIEDKVVRFPEKDHHQIFIEP
                     EGTATSEVYVNGFPSSLPEDVQLAALRTIRGMEEAVVNRFGYAVEYDFVFPHQLTPSL
                     EAKAVKGLFLAGQINGTSGYEEAAGQGFIAGVNAARSVSADPPIVLRRHEAYIGVMID
                     DLVTKGTEDPYRLFTSRAEHRLLLRQDNADVRLADRAREIGLIGGDELRRRQEKARAV
                     ESLVSRLREIPVPPEKANPVLTGKGATPITEAQSAYRLLKRPEITLDEIVRMVSEDVA
                     GIHGEIAWQAEIEVKYEGYLVRQREAAERQAAAENREIPPDFSYDAVKALSAEARQKL
                     RTVRPATLGQAARVPGITPADITVIALALEARRRRTEVP"
     gene            19156..19779
                     /gene="rsmG"
                     /locus_tag="P5179_00730"
     CDS             19156..19779
                     /gene="rsmG"
                     /locus_tag="P5179_00730"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRS93777.1"
                     /translation="MKSFERHLTDVQRKQLLGLVRLVHESNTRLNLVAPGDIPKLWER
                     HVCDSLQVLDFVDLCGRNVVDIGSGAGFPGLPVAIAVPSARITCVERIRKKAAFIRRA
                     TLELELHNVEVAWADSTELAQNSRYAGCVDIVTIRAVAHTRESLSLASGFLAENGFAL
                     LWQTRDQMLREPMPAGWMDEWFPTETDEGGARGIRKAFRATASSSLG"
     gene            19910..22999
                     /locus_tag="P5179_00735"
     CDS             19910..22999
                     /locus_tag="P5179_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS93778.1"
                     /translation="MVCARLILIVLSAAASATARDPLLESSRWRFFEDPKEIASLYTN
                     DLVQEIGGGVWLTTDRGLALHDGTQFVKKEIPGISPSARFHNLLYSADGTLWVGTSEG
                     LAKYIAHQWHYPVRQGNITALAESADGAIWVGASPADDEQFARDKRGAAPRPRTTVSV
                     YERRSWREFTSADGTFPSLPVRVLFGDQDGSVWAIFGDNNGHSEIYVYSGATWKNVGR
                     EIGLPSVEYLSLAQTRDGAIWFGTAGHGVLRWRAGSLERFVGDRFASSFTAASLLPMP
                     DGSLWAVGSPAGTLLEFRDGAWVGHSVRQVGVTGRLVSKIVYALDDAFWFVIPGRGVA
                     RFDRRGGPWWSYDAGDGLPESARVSCFARTGDGIQWVGTTHGLLLFDGKRFSDDRVPA
                     ELRGKPVSALLSFPDGRLWVAIASPVPPPKIWERSPRQTASQWRRIPPFLPSGGKSTI
                     HAFLRSRNGDVWAGVSGDVKARGVFRFRNGSWERLSGSQGAGTDDVLALAEDTAGGIW
                     AGMRNSLSRFDGVRWSAFDGQNSVRFEHISALCAARDGSVWVGGNSPNAGVSRYAGGR
                     WTHFTVDSGLASDDVWSIVEDEYRTIWVGTARGVSRYFRGQWLTFRKESGLAEDHVSA
                     STLLGDGSLLFGHFDGAASRYHATSSDPPRSFIEPLPERVAHPGFLTVRWTGSDRFGR
                     TPAKDLLYSWRLDAGEPSPFSPATAHTFEELPAGEHVFTVRTLDQEGNDDGTGVSATF
                     TVEPPFWRTPWFVVPTVVAAVGIGFATAIAYQRNRRWKRAQAQLLSELQRELQVAQKL
                     QNALLPSVDPAVPGFEITGICRAASRVGGDYFAYVWDDEDRSKFGVVVADVTGHAMQA
                     AIPAVLFSGMVTAAARQTTQPAELLELLNATFWGRASRNTYICCTVALFDGERGILQI
                     ANAGGLDPLHRSGDRVTAISVEGERLPLGVAENTRFEQCEKKLGIGDIVVLVSDGVVE
                     ARSPAGEMFGFGRAEEAVRRGSTAFEVRDSIVTEVTAHMGEHEQDDDITIIAVRVTG"
     gene            23056..23496
                     /locus_tag="P5179_00740"
     CDS             23056..23496
                     /locus_tag="P5179_00740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS93779.1"
                     /translation="MGSNAMTIELQVPSTAGYERVAMDAAASVARLVGFRQSRIEDLK
                     TAVAEACINAMEHAHKFNEAMKVVVALTVQDSTLRIDVVDQGSGMQNNPTDPDISRKF
                     SGEQAARGWGMFLIKALMDEVEFNVRSELGNVTRMIIRLEPPGK"
     gene            23559..>23808
                     /locus_tag="P5179_00745"
     CDS             23559..>23808
                     /locus_tag="P5179_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93780.1"
                     /translation="MKTDEVKRNGVATRVAGDVAILDILGDLTAQTREEVEAAYQHAT
                     ESGAKKLLLTFTKDAYINSGGIAIIINVAIAGRKHGQKI"
BASE COUNT         4937 a         6783 c         7177 g         4811 t
ORIGIN      
        1 tcgtgcaaac cgggaccctg ttcgagcaac gggatgcgcg tgggggatgc ccccgtctgc
       61 gaggaaagga attcggcgat tcgttgtctg ctttctgctc gctgactctc aagcgcttcg
      121 gcggattgct ccaacgttcg tctcttcgcg cgcattgcac gcaagagcct ccgtgcctcc
      181 atttctgcgc cggccaattc ctcccgttgc cgctcgcgcg ttgtgatgag ggtatccaga
      241 agcattttct ggcgggagta caaagccgtc aacgagtcca gccgcacctg gtcacgcttc
      301 aatgaacgta tctcatttgc gtcacgtcgt gcggccgccg tgaggtattc cctcgccgca
      361 agccagtgcg cgagcgacga cgccatcaat gcgcgctgga aaggttcgcg tcgcccgtgg
      421 atatagagag ttctcacccg cagcgaaatc gattccctcc ggcgctcgat cttctcccgt
      481 gtttgctgtg ttctgatctg cgcgctgttc atgtcggaaa caagcgcagt ttccgcttcg
      541 gtaagacggt tgatcgcgtg cttggtcaat ccggcgcggt tcgacgcagc caccacctgt
      601 gaggccaacg cccgttcacg cgctttcgcc atccgcattt ccgcgttcgt ggcggccagt
      661 tcctgctcaa tcctagccaa ctcggcctgc tggacttcca aacggttact gagatcacca
      721 tcggggttct gtggcgacgc agtaacggtt gtgcagagaa ggaagaaaga gaagacgccc
      781 gttcgacaca caagccgtat caacacaggg aattcctcgt tacctgctcg ctttcccagg
      841 ggtcacgggg gagcgaactg cgcagggtgg agatgcttcc cgcaagcccc agaacgacgc
      901 caattccaac cagcgaccat gccgccacgt gggcgttcgt tcctccaaag ctgtggccga
      961 ccagtggaat cgcgtatgcg aaacgcgaac acgcgagcac aatgagggcc gcgagaattc
     1021 caccaactgc tccctgtgca acgccgagaa ttacaaacgg gcgccgcaca aaccattcag
     1081 aagctccaag gagccgttgt agcgtcacca cttctgcgcg gctcgcaatg ccgagaccaa
     1141 tggttcgtga aatcaccacg ctgatcgcga agcccacgat tccaagaaaa agcagcaaca
     1201 cgacgttgac agtgaacgag atacgttcga gtatggttaa ccattcaacg tcggcgtcga
     1261 cggactcaac ttcggggaag ctgccagcct tccgggcgat ggccttgagg ccttcaggcg
     1321 ttcttccttc gtcactcagg gtaaggaggt aggattgagg gagaggattc gtttcgagta
     1381 catctaccag ctcggaaccg aaaaaccgtt tgaagcggac cagggcctcc gctttcgtga
     1441 taagccgcgc attgcttacg ccctcgattg caacgatccg ccgcctgacg agctgaatcg
     1501 cctccggttc gaggccgtct ttgaggaaga cctcggcggt gatccatctc tttgcggttt
     1561 gcgcttcgtt gtgaaacgcg agagcgccca gaccgagaat tccgagtatc gcgagggaaa
     1621 cagccatgat cagcacggag ctgatcgtag cccgtttcgc ggaactgacg gcacgccacg
     1681 cctcggtggt gcttgtgctc cagaacacgt tatactccct gcagacatcg ccccctcatg
     1741 ggcgattgca ggggaaaaat aaggggttgc tcaggccgag tccaaggaat cgcgttcgta
     1801 ttcctcacgt cgcaccggca aaagaacacg gaccgttacc acagtcgcgc atgattcgag
     1861 aaggcctatc ggctcgtcaa ttccgaacca tccatctcaa caccgatgtt ccgcctgcgg
     1921 aggaggaggc cggtggccac atcgagtgcg agcaacgctt tctcgtgctc cacgcgcgcc
     1981 tccacgtacg cgcgccgcgc gtccgcaacg tcccgctcaa cctgcagaac atcatagttg
     2041 gtagacagac ccaggcgcag cctttcctgt tcgttctcaa gcgttgattc ccgcaacctc
     2101 ttcgtctcct gcgcggccgc gatctgttgc cggcttgttt cgacacgtcg tagagccgac
     2161 ctcacatccg cctgcacagc ctggcgcgcg tcctgaagct ccagttctgc cgttctcact
     2221 gcaatgatgg cttcacggta actcgcttct gactgacgat tgccaagagg aaccgagaaa
     2281 cgaagcccca acgtccatga cgggtatgct gccgtgggaa gatcatccat gcttctggct
     2341 gccgttcggt ctccatcccg gagtacaacc tccgtcgagg cgtccagagt cgggagaagc
     2401 ccgttgtatc ggtatttcag agtgaatttg ctggtttcga ccgcctgtgc cgcctgaaac
     2461 accgcagggt tgttttcaag agccgcagcg aaaagtgaat cgaatgacgg cgttccagaa
     2521 tcaatgtggg tcgtgtctgt cgggactatt ccaaatgacc acccgtcctc catcgccgcg
     2581 atgtttgtca gacgtctgag ggtgtcttcg gcatcccgaa cgcgtgcctc ggcggatatg
     2641 attccctggt acgcggtggc gacaccggct tccgcctgaa ttacgtctct gagtgcctgc
     2701 gcaccggttt ccgcacgcgc acgggatcgc tccagcagtt gttgggcgta ctggtgtgac
     2761 atctgtgcga cgcgaagatt ctcacgtgcg ctcaccagat cccagtacgc ggattccacc
     2821 ttttgaacgg cggccgacgc atccgccacg atctgtttca tgctggtttc acgattcgcc
     2881 cgcgcgatcc gtatgccgaa ctcgttggcg tcccgcccga aacccctgag cagcggttgc
     2941 gaaacgctca gcgatacgtt cgacccgtaa ttcgaacttc ccgagccttt cgaccacgag
     3001 ttgttcagat tgatactggc gttggtaccg aaagaaaacg ctttggagat tcccagccgt
     3061 gtgcccaggc tgttttgcgc gggtaccgat tgcgaaatgt aggttcccgt agagggatcg
     3121 aatacgagcg aattgggctg ttgcgtacgt gaccctgtaa aagaggcggt gaacgcgggg
     3181 tcaaacggtg caagggccgc cttgagacgt gcctcgctga tcagcggttc caggcgcttc
     3241 acccggaagc tgagattgtt ctggagtgcg cgtattcggc actcctgcag ggaaaggcga
     3301 acgtattgct cttgtgaggc cggtatagcc tcaaaggcac acgcgttgag gatggcgacg
     3361 aaaacacact cggcgactgc gcggcttttc atcgatcact ccagttggga aagcagcttc
     3421 gcgtgcctcg gaacagcggc ggagggggcc gactcggatt gtatcccacg tgtggtgaaa
     3481 aatcgcgaaa tgccccacct gaatcaacgc gcggaccgac cgttttcgta tgaattgcgc
     3541 ttcatccggc ggttcggcgg tttcgggcat cgcgaatgat tccgtttccg gtgccgggga
     3601 gttatcttta ttcagcaccg taagaggaag actcccgcag gatcactgcc ggtctcctct
     3661 cgttcgattg gaccatcgag tataccccag atgctgaaaa tcaccaagaa acagcgtgag
     3721 cgctggccgc gcgaactgat tctttccgaa aagagtgaag agcttgagga gcatctcacg
     3781 cttctgaatg cagaggaccg cgcggccgtt gaacgggttt cccgggaggt cgactccaaa
     3841 atcgtcgaag cggcgcggga atccgtgaaa gaactgaaaa tgctggagga aggccggtgc
     3901 cctgcctgca gccagaaaac gcgccgtttt ctcttcagca ccatctgcga gcattgcggt
     3961 tggtcagggt attcccgccc cctgcgtgca aaggtcgtca ttcaccttcg tgacggatcc
     4021 gcgctcatct gcgaggatgt gtaccacact cctgacgaac tcatgtgtgt gacgcaggac
     4081 gtggttcgtt accgtgttcc gcgaaacgtt gttgcgtacg tcgaattcga tcacgccgaa
     4141 gaagacattc ggcagcggcg cgaacaactc gcaagagaag aacagggaca gtgttcgtgg
     4201 tgtatgaaac cggttgtgcg aacaaacgag gagacgagag tcacgttcgc ggcgttcgcg
     4261 atctatcagg aacggtttct tttctgcagt attcactgcc agaccgcgtt tcagaagcaa
     4321 tacccgaccc gcatccatcg ggattgctac caccgcgatt gcagcaagtg tgaggagtgc
     4381 cagaaacgat tcgacgatac gtcgtatgag acgtttctgg aagaggagtt cgtacactga
     4441 gttcgtcggc ggaggccgcg ttctcaattt cgcatcatgc gtcgggggat aaggcgctcc
     4501 gacaggccat cttgaacgtc ggaacaaacg aaaccggccg gaacaggcgc ttgtgctggg
     4561 ccagtcccgg tttggagagg tctccggcat cgttcacaag atcgcaacct tccattcgtt
     4621 ccgcgagcac aagtcgtgta gcttccgcga ggcctttgca ggctggatcg cttttcagaa
     4681 cgaaaaactg ccccagacct tccctgatca tgccgccgaa tgcaaacgcc cgcactttcc
     4741 cgcccaccac agctacgacg ccaaccagat gcggcgggcg gaatctcgcc gcggaccgta
     4801 tcgcactcag cgtgtaccca aaatcgaaaa ccggcacggg gtcctgacga tgcatgtcct
     4861 gccactcgcg gagaagcgcc tcgcactccg gaacgtctga ctccacaaat gcacggaact
     4921 caggctggac ttcgcgttca cacctgtgga ttcttttcct gagagcacgg aactcgttcc
     4981 cgcgaagctc aatcaatgcc ctccgctggt agacatattc atgctcgtag ggcgtgacta
     5041 cccatccgcg gcgagatccg tatgcggctc cagggaggtc agcccacaga aaccgtgtct
     5101 ctatcgggcc gttgaggcgg gcgagagaac ccgtcagatt ctccagcgcg ccttcatagc
     5161 cagatcccag gggaggcaaa aggaaatcga tgtggccgtg cccgcgaact gcccgatgca
     5221 ggagaacaaa gaggcttccg ccgtgttccc cgagcatgac cgcacgggcc ggggagtcgg
     5281 aataggccag cagaaaagga aaatacgccg cccagagcca cgggcgcgcg ttgcctgcat
     5341 aggaaagaaa cgtgggcatc tgcgcgattt cggccgcgtg gaagacggtt cctccgattt
     5401 caagcggggc gtgcaggtga tgccggtcag gctggttccc catcgccggc tgcttcactt
     5461 gaacgcgcca acgccgcggc ggcagatctc aattcgttct gatgacgggc gaaaacgatt
     5521 tctgaggacg tccaccgcgg cctccgtgtc aattgctccg caggcgaaga tatcgattga
     5581 cacgagcctg cattcagccc aggtgtgaat actcgcgtgg gattgagcaa gtatcagtat
     5641 ccccgaaaaa ccaaccggag aaaactggtg cgtgtgctcg gaaagtatgg ttgccccggc
     5701 cgccagaact gattcgcgga gaaaactcat tgaggggtcg ggagtgttca gaaggaccgg
     5761 gtcatccacc acaaagtcga tgagcagatg aactgggggc gagcaggtca attttgtgct
     5821 cctccgggtt tttcaaattc cgcccgagag tactggcggg cacgcaggaa gcgatcgctt
     5881 cggcacaggg ggcaacgatc ctccttcagt ctctccacag taatccagcg tttgccttgg
     5941 cctacctccc actcggcgga acacccgagg cagcggtaca cgcgaattac tggtcgcaca
     6001 tccgccgtat acaacggccc ttcgtaagtc tcgcgcaggg taggagcaat ctccggtgtg
     6061 gcgatcaggt gagccataag ggatacgttc gccaacacgg aggacccctc gtattcgttg
     6121 aacgcaggaa tccactcgac gattacccag ccgatacccg aaattgccgc ttgaacctgc
     6181 ctcattccgc ccgggtccag atgccccatg gaaaagaaac agtgtgctcc ttcgtgctct
     6241 acggcctctg cggcgcggca gaggaacagc tccgcgcccc ccggggtgta cggaggatcc
     6301 gtgaaaacga cgtcaaatcg gtgctgaagg gagtcgggta acggtttgcg aagatcgtga
     6361 acaaaggttt gcaccgaagg cgcagccttt ttgatcccgt cgcatacgcg tccgtcgata
     6421 tccacaacgg ttagcgttgg tgtcgggccg gccggttccc caccggcgca ctgatggacc
     6481 catctcgcga ccgcgacaga gacaaaatcg tcgtcgccaa gaaagagaat ccggcgaccc
     6541 cacagggaat cgttcttcag aagatagttc acacgccgga cgagggttcc gacggtagcc
     6601 tttgcctggt ccagggtcac gtcagcctgc gggcgcagag cggcgagatc atccaacacg
     6661 gcggagagcc cggttgtctc gtcatgcccg gcgtcgccac agatagtctc cacacacgaa
     6721 gcgcccgctg acgtaagccg cactccgttc ggagcccgtt ccgtcacccc gcgggcggag
     6781 agctcgccga tgacggccac ggtgacaggg agcggcaggc ggaccttcga agcgagaact
     6841 cgaacgggaa ccggctccag tgtggcgatc gctcgaagga ttgtgcgaac gccctcacga
     6901 ccttcccgaa gatgcgaggc agacgctacc tctgacagga tttggccgcc gtcgccggcg
     6961 tgaagctgct catgaccttc ccggagcgcc atatgtgctt ccaaaagaga acccgcgcgt
     7021 gccggtgccc ggtcggtcct ggcgcgagga tcgcctttcc cgctcttgag ggagtgctgc
     7081 ggccctgttg gcgcgtgcct gctcgcgttc aatgtcgtcc tcggtctgga gcgctgcgcg
     7141 ccactcgtca aagcgacttg atgcgaacag cgcagccagc cctccggcaa tgtccgcgag
     7201 ggattcctgt tcctgcctcc gcgcgccgcg cgccccgaac tggcccccct gtggttcact
     7261 gatcattttg cgtgccagtt tatccgaggt tctgatatcg agcaacggca ggcttcctgc
     7321 gcgcagtgtg ttcacgaact ttatgaagcc cggcaattcg gtgcgacgca cgcgcatgtt
     7381 gccgtctcct cggatgcaga gaatcggctt gtctgggaga ttcatcagaa ggggatattg
     7441 ctcgcgaagg cggggccacc agtacgtcca gtaggacggc ggagctatgg taataagacg
     7501 gttcctcacg ttcgggttcg acaggtttga attgtcgaac acaaacgaca gcctcggtcg
     7561 tatgccctgc tcgtcatgtc cgaacgggaa gagagttacc ccgcacagtc tgagtcttcg
     7621 ttcgatgata cgcgcttcgc cgggctgcag gtccaggttg atgaaagcac cggtgagttc
     7681 atcgggcgct atctcaaact cccaaccggc tacctgggca agcgcgacca gttcgtcccg
     7741 cacctgtgcc tggcgcgata gactgaattc ctgtgcctcc cagaaaatcg tgcatcccat
     7801 gggaatctac tttcctctcg tgacggcaga tgccgacttc ggcccggaaa cacgagcgaa
     7861 gcggcgccag tgaacgatct gtctcgcgag attgcgtgtt gaaaagcaag ggaaaaggaa
     7921 gtaatcaaag ataacctctg agcgggaact caacaaggtg ccgtcatagc gctgtacacc
     7981 cagtccaagg gtacaggtat ttttgcgact cccaattccc tatcttttac tgcaaagtat
     8041 ggcgggaagc gagaatcagg tttcccgcgg agcgcgactt tcgcgtcgtc ctcttcgtgc
     8101 cggcaggagg tttgtcgtta tggtttcgga tgcagatttg ctcgacaaga ttaccgatta
     8161 tacccggaaa cagggcggag acacggcaat cgagagactt ctcgcgaatg tcgtatccca
     8221 ggtgcgtgaa ttcgcggagg cccgggcttc agaaataaag cggctctcgg acatcggaat
     8281 tgccctttcc gccgaacgga acctcgaacg cttgcttgag cgcatcgtgg acgaagcgcg
     8341 acgatttacg cgggctgatg cggggacgtt gtacacggtc cagatcaatc ctgcgaccaa
     8401 tctgccggag ctgaagttcg atatcatgca gaacgacacc atgaacaccc ggcagggtgg
     8461 aacgagcggg aatccgataa cgcttcctcc ggtgccactg gtcaaagacg gaaaaccaaa
     8521 ccacaacaac gtttcggcgt acgttgccga caccggcgaa acggtcaaca ttcccgacgt
     8581 gtatatcgcg gaggggtttg actttaccgg cccgcgcaag ttcgatcaga tcaccggtta
     8641 ccgaactaag tccatgctcg tggctcccat gaggaaccac gaaggagaga ttattggcgt
     8701 tctgcagttg ctgaatgcga aagttccggg cggcgaacga gtgattccgt tctcggacga
     8761 gtacgtggat ctgatcaaga gcctcgcgtc gcaggctgcg gtcgcgatca ccactgccag
     8821 gctcattcag gatctccaga acctttttga gtccgttatc cgcctggttg cctccgcaat
     8881 cgacgagaaa tccgcgtaca ccggcggaca catcactcgc gtggcggggt tgtcgatgaa
     8941 gatcgccgaa gccatcaacg agacgacgga aggggatttc gcggatgtga agttcacacc
     9001 cgacgaactg aacgagcttc ggatcgccgc ctggttgcac gatatcggca agatcaccac
     9061 accggaatac gttgtggaca aagggacaaa actggagacg atttttgacc ggattgaact
     9121 cgtcaagacg cgcttcgagg tcatcagacg cggtgtgatc gcagatgcca tggaatacaa
     9181 aatgcgtctt attgcgaatg gcgccgatcc cgaggctttg aaagcggttg acgagcaact
     9241 ggcagtaagg cttgaggaga tcgaaagcga taaggagttc gttgcgcgct gcaannnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggcag taaggcttga ggagatcgaa
     9421 agcgataagg agttcgttgc gcgctgcaac acgccgggcg agttcctcac ggatgacgcg
     9481 atagaccggt tgaaggcaat cgccgcgaag acgttcctgc ttgacggggt ggaaacaccc
     9541 tacctcaccg agaacgaact gaagaatctg agcatccgca aagggagtct caccgaagag
     9601 gaacggcaga tcatcaacaa ccacgtttcc gtcacaatca aaatgctttc gcaattgccg
     9661 ttcccgaaga aactttccca tgtcgtggag tacgcaggtg cccaccacga gaagttgaac
     9721 ggtgccgggt accccaacgg gtggaccgca gagcgcctgg caatccaatc gcggattctc
     9781 tgtcttgccg acatctgtga agcacttacg gctgcggacc ggccttacaa accgtccatg
     9841 ccgaaagaac gcgcatttca gattctcggt ttcatggcca aagatcagga aattgatgag
     9901 aggcttttga agttgttcct ggagaaagag gtctatgacc gctacatggt ggaatacaaa
     9961 gcgcagaagg aagcagaagc cgcggcagcc acggccaaat gaagagccgg ctttccgaat
    10021 gtgaccggaa agtcgaggga cgcgatgacg agcggatcgg tgaacccgat ccgctttgtc
    10081 atgatgggtt ttcaggggaa tgaacagaca gagcgaagga gattggaaca atgtcgaaac
    10141 cacgtctggg gttcatcggg ctgggcataa tgggacgtcc gatggtaagg aacctcttga
    10201 aagcagggta tcaggttact gtgtggaaca ggagtgaacc cggtatcgcg gcgtgcgtga
    10261 aggagggagc tcagaaagct gcttcaccgg ccgatgtcac ttccaggtgc gacatcgtgc
    10321 tttcctgcgt cacagattcg ccggacgtca ggcaggtgta tctcgggccc aacggtgtta
    10381 ttgataccat acgaccgggg atgatcgcca tcgacatgag taccatcagc cccgcagtag
    10441 cgcgcgaagt ggcgtccgct cttgaagcga agggcgcaaa aatgctcgac gcgccggttt
    10501 cgggaggaga aaaaggcgca atcgaggcga cgctgtccat catggtgggt ggcgacgccg
    10561 aaaccgttgc caccgccaca ccgatccttc aagcgcttgg caaacgcatc atccacatcg
    10621 gccccaacgg catggggcaa acggtgaaac tgtgcaacca gatagtgggt gcactgaacg
    10681 acctggcaat gtgtgaaggg ctgattcttg ccgcgaaatc aggggctgac gtcagcaaaa
    10741 tggtggacgc cgtctccgcc ggggcagcgg gctcgtgggc gatgagcaat ctcgcgccga
    10801 agattctgaa aagggatttc tccccggggt tcatggtgaa actccagcag aaggatctcc
    10861 gtctcgtgct ggataccgcg aaggagcttc agatcgcgtt gcccggaacg gctcttgtca
    10921 accagctttt ccagagtctg gaggggaaag gttgcggcga cgaaggcatc caagcactgg
    10981 tgaaagcgtt ggaacagatg gacggcgctg aggttcgggg ctgaaccgcc ccgaatcgaa
    11041 ggcgccgaag ttcagccaga ccttgcggcg ggttgctgcg cgccccgtgt gcgttcagga
    11101 ctggtgcttt ggcgagctga gggccgttcg gtagagctgc tcgtattccg cgacgatctt
    11161 gtcggccgag aataaccggt ggacgcggtc cagagcggcg gctcggattc gggcagccag
    11221 ttcctggtct ttgagaaccc tggttgccag ttctgccatc atttcggtgt caccgacctc
    11281 aaccagatgc cctgacactc catcttcgat cacctcgacc agaccaccag cccgggatgc
    11341 gatcaccgga acctcgcacg ccattgcctc gagagctgcg agaccgaagc tttcgtgttc
    11401 gcttggcaaa aggaagaggt ccgcgaggct gagaaaaagc tccaccctgt cctgttttcc
    11461 gaggaaccag acatccccca tcacaccgag ctccagcgcc acttcctgcg cgggcatgcg
    11521 gtgtggtccg tcaccgatca tcaagagctt cgccgccatc cgtttgcgta ccaatgcgaa
    11581 tgccctgatt acgtctgtta cccttttgag cgggcggaag ttcgaaatgt gaacgaggat
    11641 aggttctcct ccgggggcgt aacgggctct ctggcatgca ggcaccgaga ctcggcggaa
    11701 tctctccgtg tccacggaat tgtaaatgac ctcgattgga tgggccacct tgaaccgtga
    11761 aagcgtctgg tccttcagcc actggctgac ggcagtcacc acgtcagatt gttcgatcga
    11821 gaacctggta atgctgaagt atgaagggtc ctgcccgacg agcgttatgt ctgttccgtg
    11881 aagcgtagtg acgagccgga cgttgtggtc agggcagagc ttcagagcaa gataggccgc
    11941 ggttgcgtgt ggaatcgcat aatgaacgtg caggagatcg agcttctccg tgcagatcgt
    12001 ttctgccatt ttagcagcaa gcgaaagcgt tgtcggtgga tggtcgaaca acggatagtg
    12061 cataacgtcc acttgatgga ggtagatgtt cggatcaaac tgcaaccggt aaggctcggc
    12121 gaatgttacg aaatgaacct gatggccacg tttggccagc gccattccga gctccgttgc
    12181 caccgttccc gatccgccgt acgtgtgatg gcacgtaata ccgattcgca caacgtcctc
    12241 cgtttcatgg gttgctcaac accaatgctc ttccgttcgc cttcttcgtt ctttctgtct
    12301 ggcgcgaagc gcttgcacac ggtcctcaac ttcgcttgcg tgcgacgcga cacggtcctg
    12361 cctgagctgc cggtaaaacc ggtgcagagg tactctcctc gctgatggca acacgccggc
    12421 gacttgcatc agcgagcgca catcgccgcg aaagagcgtg ttcacaagcg agcccaaccc
    12481 catttcagtg agtgctcgtg acgcgagcgt tccgcttcgc aacgcatctt caacgcgggc
    12541 gagttcgatc ccgaggcggg agagcgccca tattgccgct tcccggacgc tcgagcgagc
    12601 gcgccagtag gaattcggca cagaaccaag ctcccaacgc gcgaatcttc ccggaaacac
    12661 gtcgtctatt acccgccaga cacttcccat ctgcctcaga aggccatcca aacctctcat
    12721 cttgaaaacg tcaggcgaga gtaagccgtt cctgatggca accggaatct tctccacttc
    12781 gacatgatac tctttcagca gccattggac cgcgttggtg cgcagcgccg ggtccttcca
    12841 cgcctcgcgg ggcacatggg tcacttccca cagttcgaac gtgttcggga acagagaatt
    12901 cagaagagcg aacgagttct cagaaaatcc gattcgaaac gcggcgccca gatgccagcg
    12961 gttcagcgtg gacttgatga cacgagcggc atcctgcgtg cggaatgtcg ctacttgcat
    13021 accttcttcg cggaatgccc agccgattgc ttccgaggaa aggaggcgcc cggcgtcgcc
    13081 gtgccaggtt tccaccggca cgcgacagaa gcactcccag gggcggagct gcgggtacga
    13141 ctcggcaagc gcgtgtgcga ccgaccagcc atagacgttt ttcagcagcc acgtcaatcc
    13201 agcttcgcga aacgccttct tggaaatggt gttctggtgc acagcccgtc cgatagcttc
    13261 gggtggaatg ccgaggcgct gttctacaag ccatttcacg gcgtcccggc gatttgcggt
    13321 ttcttcccag tagcctgtgg gaacgtggct gcccatccat ggcttgattc gttccggaaa
    13381 cccaagcgca agaagcctgt tcaccgagcc cccgcagcgc cgcaaagctg gtcccaaccc
    13441 aagggcgatg tacgaaagcg catcctcctg cagtgcctcc tcggggctct ttcgcatttc
    13501 gcgcatcacc aggtagttca ccgcgattcc tgtgcggcgg gtgtcttttg agaagaaacc
    13561 ccacggaaag gccgcggcaa gacgtgcggc tacgtatcgt gcgtggagga ttatctcgtc
    13621 atcagacatc cggtccgtta cgggagcgtt gctcaagggc gggatactgc cgtcaggaaa
    13681 gtgacgttgg aggcgttcca gcctctccgc gagcagatcg gttgcgatcg ccgacaggcg
    13741 ggcagtggga agcctttcgg ccgcgttctc caggagcttt ttgaagtacg accgcaggtg
    13801 cggggggaga gcaagggccg cctgctccag ttgaggagag tccagggatc gctcgggttc
    13861 cgcagcgttg ctttcaacgg caggagcatc agaaagagga gagaagaaac gcttgctgat
    13921 ctccgggaaa aatgtcctcc cgacctcgtc cgctgcttcc agcagaacgg gacgatacag
    13981 gcttcgatcg tagggggtgg aaagaatcag gcgaaccacc tgctccgtgc cgacgatgga
    14041 ttgcgcaatg tcctcggtgg catcctcgca gtcgcctcct gtcgcgcgga ccaaagcgga
    14101 tagccgctct gacacgatct cgcgcgcctg gttaagggtc tgtcggtatg tcttctcgat
    14161 caaacccgaa gacggcatat cctgttctcc cgctttgtcg cttcagcggc tcatcgtgcc
    14221 agaggtatca gcgcttccgg cgggatatga acgtatacct gcgaatcctc ctccggcacg
    14281 cgggattggg agaagcgctg gagaatcttc agcctcaggt tcccgtcctg aacgcttatt
    14341 tccatgtccg cgccaaggaa tcgtcgacca gagactctca tcaacagggt gttttttgag
    14401 gagatgggag tggtatccag tgtcatttca tgggggcgaa cagccagcgc ggccgtcggt
    14461 tcctgcggaa gagttctcgt tccaaataag gtgtggggac ctgtctgagc cgtccagccg
    14521 tttcccgcct cttgaatatg ttctacgggt aggatgttgg tgtgcccaat gaactgcgcg
    14581 acggcaagag agcccggact ggcaaaaaga tcctgcggcg caccgacctg aatcagcctg
    14641 ccgtgttgca tgatgccgat tcgatcgcac agcgcgaagg cttccgcacg gtcgtgggta
    14701 acgtagaccg tcgtgatccc gagtcgtcgc tgaagtgccc gaagctcctg gccggtttct
    14761 tcacggagcc gcgcgtcaag attggaaagc ggttcgtcca gcagcagcac ggtgggctcg
    14821 atcaccagcg ctcgagcgac cgcgacgcgt tgttgctgtc ccccggagag gtcagctacg
    14881 gaacgccgtt ccatcggtgc cagctgcaca agctccaacg cgttccgaat gcggcgaccg
    14941 cgttcctttc tcggaactcc gcgagccaga aggccgaact cgacgttctc gtgcacagtc
    15001 atgtgcggga agagcgcata actctggaat accatcccga cgttgcgttg ttcagggcgc
    15061 ttgccagtga tgtcttcgtc tccaaaaaac acgtggccgt gctcaggttg gatgaacccc
    15121 gcaagaagac gcagagtcgt tgtttttccg cacccggaag gccccagaag accgaacaac
    15181 tccccggatt ccacctggag attcaggttc gtgactccgg cttccccgtt gtaccggtaa
    15241 ctgagatccc tcaggatgat tctcatcggc acgccgcctg aatgcgttat tcggttacca
    15301 cgagatcgac ccggtcactg gcgaacaccc gggttccggg agcaatggac tgcttcagaa
    15361 ctgtccccgg cgcagcggtc tcatcgggtt cggaggtcgt ttctccaatc tggattccca
    15421 tagcggcaag ggttcgactt gcttcaccga ggtctcttcc caccagagca ggcatctcgg
    15481 gagcgatgct caccacgagc cttacctgag cgtccttttg cagcgaggtt cccgcatcag
    15541 ggaactggtc ctgaacatag ggttcagaca ggtcctcgtt tgtccggagc acgtaactga
    15601 cgttgccgac tctgaaccgc gcctgttcaa gcctgacgcg ggcgtcgttg agtgaaatac
    15661 cccgcacatt gggtacaacg tactttggag cgcctctgct gacggttact cgaacgctcc
    15721 gtccgcgctt cacagaaacg tcgggagcgg ggctttgcat gactatgtga tcgacaggga
    15781 tggattcatc cgtggcatag tccgacgagt cattcacgaa taccagacct tgctcctcaa
    15841 ccagacgttt tgcctcggcc acgtccattc cacggacgtc tggaacgaca acgtccgttc
    15901 cgcgggtgat aagcggcatt actacccagt tgcccagaac gaaaatgagt aacaacgcga
    15961 gaaaggcacc tgtcgcggca gcggtgggga gggtaagaaa ccgttgaatg cctttggccc
    16021 cggtgttcgc ttccgtgctc ccggtgacgg ccggcctgtc atctggagtc atcggacgct
    16081 gcctcctgcg cgtaccaagg ccattcgggc cgccagctga attgcatcca gcatactttc
    16141 ggtcctcgcg attccctttc cggcgatatc gaaggctact ccatggtcga cggaggttcg
    16201 aacgatcgga agcccgaccg ttacattgac tccgtgaact gatgcccatc ccccgtcttt
    16261 tgaaggctcg aaggccaggg ttttgacggc tatatgcccc tgatcgtggt acatcgcgag
    16321 cacaccgtcg tattccccca gccgggcgcg agcgaacacc gtatctggag ggattggccc
    16381 gagtacctcg acgcctctcg agcgcgcctc gagaacggcc ggcaggatat gctcccgttc
    16441 ctcatcgccg aacagacctg cttcacccgc atgggggttg agcccggcga caccgatccg
    16501 aggcgacgcg atgcccagat cacgcaacac accgtcgaac agaagaagca tccgcaggac
    16561 gcgctcctgt gttacgcggc ttattgcatc gcggagcgag caatgcgtgc tgacatgcag
    16621 aacccgccac gtaccgtgca cgagcatcat gccgtattcc ttcgttcccg ttttcgccgc
    16681 caggaactcc gtatggccgg gaaaagggac tcctgcgaga tgccacgctt ccttggatat
    16741 cggtgccgtt actatcccgt ccacttcgcc cttctgggca aggactatcg cacgatccag
    16801 atacagggca gagagtctgc ccgcctctgc cgatacggca cccggcggga cagcgacctc
    16861 gggttccgcg acgctgcaga tgtgtgcagg gtcaggaagc gggcgcccga gggttttcgc
    16921 gacgctctgc agtatggact cggttcccac aaacaacagg tcggccgcac ctgtcgccct
    16981 ggtatcagtt gccaccgcga ggcagagctc cggtcctaca ccagcgggat cgcccatggt
    17041 caccgcgagg cgaggtttgc tcacgttcat cttggggtat gtcctgcatg tcgttggagt
    17101 tctggtattc ggttacatcg tctgaatgta cccgcgcgag gggacgcagc caaatgtcgc
    17161 gtcctgatcg catttagcga cactggtcgt tccgtgctat ttttactctg ttgactgcca
    17221 cgggtttcca tggcggcaag tggtgagtct gcagcggttt gttgggcgaa cgaaaggcac
    17281 ataattgctt tcctgcgata tcgtggttat tggtggcggt cacgcgggca tagaagctgc
    17341 gatggccgct gccaggcttg ggttcgacgt ggtcctgatg acaatggatg tcggaaaaat
    17401 cggggaaatg tcgtgtaatc cggcgatcgg tggcacggcg aaaggacatg ttgtccgcga
    17461 gatagacgcg ctcggcggtg tgatgggcaa gttgattgac gccacgggta ttcagttcaa
    17521 gatgcttaac cgttccaaag gaccggcaat gtggtctccg cgcgcccagg ctgaccgggc
    17581 cgcgtacagg gccgcggcac aggcccttgt gtcctcacac gaacgcgtca gaatcgtggc
    17641 cgagcaggca ggccaactga gaattgaggg aggccgcatc gtcggagtga tcggcgtctc
    17701 tggaaccgag atttcgtgca gggcagcggt tgttgcgact ggcacatttc tcaacgggtt
    17761 gatgcacgtc gggttgtcgg cttccattgg cggccgtgtg ggggaaccgg ctgcctcaga
    17821 gcttccgcga agccttgcgg cgggagggtt ggcactcggg agactgaaaa caggcacacc
    17881 gcctcgcgtt gacggccgca caatcaacta ctcggtgatg actcctcagt acggtgacga
    17941 accgccgcct ttcttctcgt ttgaaacgcc gccgcgatca gtgcaacagg ttccctgctg
    18001 gctcacctgg acaaacaccg caacgcacag acttatcctc gggggattgg accgttctcc
    18061 tctctacacc ggcaggataa aaggaaccgg tccgcgctac tgcccttcca ttgaggacaa
    18121 ggtggtgcgt tttcccgaaa aagaccacca ccagatattc attgagccag agggcacggc
    18181 aacgtcggaa gtgtatgtca acggttttcc cagcagtctc cccgaagatg tacaacttgc
    18241 agccctgcga acgatccgcg gaatggaaga agccgtggtg aaccgtttcg gctacgcggt
    18301 cgaatacgac ttcgtgtttc cccaccagct cacaccaagt ctcgaagcga aggcggtgaa
    18361 agggctcttc ctcgcgggcc agatcaacgg gacttcggga tatgaggagg cggccgggca
    18421 agggttcatc gccggggtga acgccgcgcg ttcagtttcc gccgatccgc cgatcgttct
    18481 gcgccgtcac gaagcgtaca ttggcgtgat gattgatgat ctggtaacca aagggacgga
    18541 ggacccctat cgcctgttca cttcccgggc ggagcatcgt ttgctccttc ggcaggataa
    18601 tgctgacgtg cgcctggccg accgagcccg ggaaataggc ctgatcggag gggacgagtt
    18661 acgacggcgg caagagaagg cgcgggcggt tgaatcgctc gtttcaaggc tgcgagagat
    18721 tcctgtcccg cctgagaaag ccaatccggt gcttacgggg aaaggcgcca ctccgattac
    18781 ggaagcgcaa tccgcatatc gactgctcaa acggccggag atcacgctcg acgaaattgt
    18841 gcgaatggtc agcgaggatg tggcggggat ccacggggaa attgcctggc aggcagagat
    18901 tgaggtcaaa tacgagggct acctggttcg gcaacgagag gccgccgaac gtcaggccgc
    18961 ggcggaaaat cgcgagattc cgcccgactt ttcgtatgac gcggtgaaag ctctgtccgc
    19021 ggaagcccga caaaagctgc gaaccgtccg tccggcgact ctcggtcagg cggcaagggt
    19081 acccggcata acgccggccg acattaccgt tatcgcactc gcgttagaag cacgaagacg
    19141 acgcacagag gtcccgtgaa atcattcgag cgtcatctca cggatgtcca gcgcaagcag
    19201 cttcttgggc tggtccgcct tgtgcacgaa agcaacaccc ggttgaacct cgtggcgccg
    19261 ggggacattc cgaaactctg ggaacggcat gtctgcgact ctctgcaagt actggacttc
    19321 gttgatcttt gtggaaggaa cgtcgttgat atagggagcg gggcgggttt tccgggtctg
    19381 cccgttgcca ttgccgttcc gagtgccaga attacctgcg tggaacggat ccgaaaaaaa
    19441 gccgcgttca tcagacgggc gacgttggag ctcgagcttc acaacgtgga ggtggcgtgg
    19501 gcggactcta cggaactcgc ccagaattcg cgctatgccg ggtgcgttga tattgtgact
    19561 attcgcgctg tggcgcacac gagggaatcg ctttcccttg ccagcgggtt tctggcggaa
    19621 aacggatttg cgcttctctg gcagacacgt gaccagatgc tgcgcgaacc aatgccggcg
    19681 ggatggatgg acgaatggtt tcccaccgag acggacgaag gtggcgctcg aggaattcgc
    19741 aaggcatttc gcgcaacagc cagttccagt ctcggttgat gcaaccggaa cacggcggaa
    19801 caggcgtatt gtttctttca cacgcatgac gacttccgga tccggcgacg acctgcggtg
    19861 cgggttgctg agagtgcacc gccgatcaaa gattggagcg tcccctttga tggtttgcgc
    19921 acgtctgatc ctgatcgttt tgtccgccgc tgcaagcgcc accgcgcgag accccctgct
    19981 ggaaagctca cgctggcgtt tctttgaaga cccgaaagag atcgccagcc tgtataccaa
    20041 cgaccttgtc caggagattg gcgggggcgt gtggcttact actgaccgcg gtcttgcgct
    20101 tcacgacggc actcagttcg tgaagaagga aatcccagga atcagcccgt ccgcacgctt
    20161 ccacaacctc ctctattccg ccgacgggac tctctgggta ggcacgagtg agggacttgc
    20221 caagtacata gcccaccagt ggcattaccc tgttcggcag ggcaacatta ccgccttggc
    20281 ggagtctgct gacggagcga tatgggtggg tgcctcccct gccgacgatg aacagttcgc
    20341 gcgggacaag cggggcgcag cgccgcgacc gagaaccacc gtaagcgtgt acgaacgcag
    20401 gtcctggcgc gaattcacat ctgcggacgg aacgttccct tccctgccgg tgcgtgttct
    20461 tttcggtgat caggatggct cggtgtgggc gattttcggg gacaacaacg ggcactcaga
    20521 gatctatgtg tactctggcg ccacgtggaa gaacgtgggg cgcgaaatcg gattgccgtc
    20581 cgtggagtac ctcagcctcg cccagacgcg cgatggcgcg atctggttcg ggaccgccgg
    20641 tcatggcgtt cttcggtggc gggccgggtc tctggagagg tttgtcggag acaggtttgc
    20701 gtcatcgttt acggcggcgt ctcttctgcc catgccggac gggtcgctct gggctgtcgg
    20761 gtctcccgca ggcacgctgc tggagtttcg cgacggcgcg tgggtcggac attcggtccg
    20821 gcaggtgggt gttaccggca ggctcgtgag caagattgtg tatgcgctgg acgacgcgtt
    20881 ctggtttgtg atcccgggcc ggggagttgc gcggtttgac cgccgcggcg gtccatggtg
    20941 gtcatatgac gccggcgatg ggctgccaga gtccgcacgt gtgagttgtt tcgcccgtac
    21001 cggcgatgga atccagtggg tcgggaccac acacgggctg cttttgtttg atggcaagcg
    21061 gttttcggat gaccgggttc ctgccgaatt gcgggggaag cccgtttcgg cgttgctgag
    21121 tttcccggat ggaagattat gggttgcaat tgcgtcaccg gttcctccgc cgaagatctg
    21181 ggagcggagc cctcgccaaa ccgccagtca atggcgaagg attcccccgt ttctcccctc
    21241 aggtggcaaa tcaacgattc atgcgttctt gcgctccaga aacggggatg tatgggcggg
    21301 agtatctggc gatgtcaagg cgcggggtgt attccgcttc agaaatggat cgtgggaacg
    21361 actgtcgggt tcgcagggcg cgggtaccga tgacgtgctc gcgctcgcag aggacacggc
    21421 cggcggcatc tgggcaggga tgcgtaacag tctgagtcgc tttgacggtg tacgatggag
    21481 cgctttcgat gggcagaaca gcgtccgatt cgagcatata tcggcactgt gcgccgctcg
    21541 cgacggatcc gtctgggtgg gaggcaacag cccgaacgcc ggagtcagca ggtacgcggg
    21601 aggaagatgg acgcatttca cggtggatag cggactggcg agcgacgacg tctggtccat
    21661 tgtagaggat gaataccgaa caatatgggt agggacggca cgtggtgtca gccggtattt
    21721 ccgtggccag tggctcacgt tccgaaagga aagcggactt gctgaggacc acgtgtccgc
    21781 atcaacgctg ctgggggatg gctcgttgct gttcggacat ttcgatgggg cggcgtccag
    21841 ataccatgcc acgtcgtcgg atccgccaag gtctttcatc gaaccgttgc cagaacgggt
    21901 tgcgcaccca gggttcttga ccgttcgctg gactggaagc gatcggtttg ggagaacgcc
    21961 ggcaaaggac ctcctgtatt catggcgact ggatgctggt gaaccgtccc ccttttctcc
    22021 cgcgaccgcg catacgttcg aggagcttcc ggccggcgag catgttttca cggtcagaac
    22081 cctggatcag gaggggaacg atgatgggac gggcgtttcc gcgacgttta ccgtcgaacc
    22141 acccttctgg agaacaccct ggttcgtcgt tccgaccgtg gttgccgcgg tgggaatcgg
    22201 tttcgccacg gccatcgcct accagcggaa tcgacgctgg aagcgtgcgc aggctcagct
    22261 tctgagtgaa ttgcagcggg agttgcaggt tgcccagaag ctccagaatg ctcttcttcc
    22321 gagcgtagac cctgccgttc cgggcttcga gatcacgggc atctgccgcg cggcaagccg
    22381 ggttggcgga gactacttcg cgtatgtttg ggatgacgag gatcgatcga agtttggtgt
    22441 tgttgtcgcc gatgtgacgg gccatgccat gcaagcggca atcccggcgg ttctgttcag
    22501 cggaatggtg actgcggctg cgcggcagac gacgcaacct gctgaattgc tcgagttgct
    22561 gaacgcgacc ttctggggcc gggcgtcccg aaatacctat atctgttgca cggtcgctct
    22621 gttcgatgga gaaagaggta ttttgcagat agcgaatgca ggaggattgg atcctctgca
    22681 tcgttccggt gatcgcgtaa ccgcgatatc cgtcgaaggc gaacgtctcc cgttaggcgt
    22741 cgctgaaaac accaggttcg agcaatgcga aaagaagctc gggattggtg acatcgttgt
    22801 tctcgtctcg gacggtgtgg tggaagcgcg gtctcctgcc ggggagatgt ttggttttgg
    22861 gcgagccgag gaggctgtca ggagaggtag tactgcgttc gaggtccgtg actcgatcgt
    22921 cacggaagta accgcgcaca tgggtgaaca tgaacaagac gatgacatca cgataatcgc
    22981 ggttcgcgtt accggttgat accgcgctac gcgccgtgga cttaaggtgg ttacctacgg
    23041 gacagggggt caggtatggg atcgaatgcc atgactatcg aacttcaggt tccgagtaca
    23101 gcggggtacg aacgggtcgc gatggatgcg gcggcgtccg tggcacgact cgtaggattc
    23161 cggcaatcgc ggattgagga tctcaaaacg gcggttgcag aagcctgtat caacgccatg
    23221 gaacacgccc acaagttcaa tgaggctatg aaagtagtcg tggcactgac cgttcaggat
    23281 tcaacgttac ggattgacgt tgtggaccag gggagcggca tgcagaataa ccccactgat
    23341 cccgatattt ccaggaaatt ctccggagaa caggcggccc gcggatgggg catgtttctc
    23401 atcaaagcgt tgatggacga agtggagttc aatgttcgtt ccgaactcgg gaatgtcacg
    23461 cgcatgatca tccggctgga acctcccggg aagtaacgcg tatacgccct gaaccggcag
    23521 cagttgcttc agaactcgcg aattaagggg accggaacgt gaaaactgac gaagtgaaga
    23581 gaaatggggt tgcaacccgt gttgctggcg acgttgccat cctggatatc ctcggggacc
    23641 tgaccgcgca gacccgcgag gaagtggagg ctgcctacca gcacgcgact gaatccgggg
    23701 caaagaagct cttgctgacg ttcaccaaag acgcatacat aaacagcggg ggcatcgcca
    23761 tcattatcaa cgtcgccatc gccggacgga aacacgggca gaagatcc
//
LOCUS       DAOTNG010000007        23683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2216_length_23683_cov_4.690240, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000007 DAOTNG010000000
VERSION     DAOTNG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 23683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23683
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_23683_cov_4.690240"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2372)
                     /locus_tag="P5179_00750"
     CDS             complement(<1..>2372)
                     /locus_tag="P5179_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93781.1"
                     /translation="THLCMHFHCDPIFTCPQATHIEESVELAAQYVSMCREDPEFRCI
                     LSETDYLHGYWNLYAEDREFLLRLMAEGRVETSGSYSEPNENSVGGEALVRNILYGQW
                     FQVGYLGGRGDIYMPHDVFGHVRQLPQILRKAGFIATTWTKGSPKPGAPDPCFQWLAP
                     DGSSILTRVTSYGGVDWGASDQIGGVLKAGDAYPGDAPIPNLLFRGRDFVSPPGWISG
                     KGSALRDHRVLMSQPSAFFEEVSARIKAGEFHPPLTAREHSQYHVGTALSRIELKIAM
                     RIAEEALGDAETWSGIATLYGHPYPEAEIDHAWRQLLFSQHHDSITGTSNDQSYLDLL
                     THLREALEEGISARRTATSNLAEEITTDGRTGTPVVVFNSLGHTRTDVVEVPLPHAFT
                     GTRVRALNDTGVQTPVAVVQRETGKSARFLAEHVPGLGYSTYWLSDGDAEDSPREEVI
                     ESDDAPLVLETPFARITVDPLRGGGIVSLIERKSGREIASGASPYPLNDFVLLKEGPG
                     VEPSWEFCTTGDRVFASSIRAHVRAERTALGWRVVIRQPFPECDHLVRTLELFDHTPR
                     IECSVILSGYAGSRLVGSDTNTRPWGGSNEGKNPEPVDRDMFGVLFPVGLRGLAPVWS
                     DRFGQRVIRRSRSFLDFRTHQGRIYSGCAMFSAEKWIATSPSVSLVGESGDGSRVGIP
                     LGMVRVIYPDDPVAREAADLIVVALAEQGVTATPYLDTENQNDDVLQTDQIVAIGGPE
                     SNAWVATHREWQPIGNRFLTSVSLAEDASRRVPVLILGGGVNGAGLFREL"
     assembly_gap    2373..2472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2971..4134
                     /locus_tag="P5179_00755"
     CDS             2971..4134
                     /locus_tag="P5179_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93782.1"
                     /translation="MALMIDFDTHNAEVEAVWKAYRERKPTRVPMVFGINPRLTMFDH
                     PSNPRGITFEQYSSDPQLMMERQIEHQAWVRTNILQDAPMGLPKDGWSLWVDFQNYYE
                     AAWYGARIRYWPGEVPDTEPLLEDDASKWSLIEKGMPDPFTHGSAQRMWDYYEYFCRK
                     KEEGWTYLGLPIATVTWPAGGTDGPLTLCCNLRGASRFIPDMIEDPEYAHAMLNYITE
                     TSVARIKAYRERMGAELKPERGGLADDSVMLISTPMYREMILPYHRRIFEELAGKGPH
                     SIHLCGDATRHFKTIRDELNVWSFDTGYPVDFGWLRRELGPEVEIYGGPSVPFLRAAT
                     PDAVRAEVTRILGSGIMEGGRFVLREGNNLAPGVPMENLRAMWDAVHEFGTYA"
     gene            4149..4931
                     /locus_tag="P5179_00760"
     CDS             4149..4931
                     /locus_tag="P5179_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HRS93783.1"
                     /translation="MYKLIACEVARPEFDHVISMSRKSVSVEYLEQGHHNDVVRGRKA
                     IQERIDAASEGEWEALLLGYALCNNMLVGLQARVKPLVIARAHDCITWFLGSKERYAS
                     TFEEAPGTYYYTAGWLKVVRSHDQAGQSASASGVLVEGEYARLVEQYGEDNAAYLWEM
                     SNAWIKNYRYGAYIAVPGIDHDGHRKRVQEICARNSWEYKEIEGDLGLLQRWVDGEWD
                     AKDFLIVKPGDTIAPTLDAQIISVGRKEGGQNGRIDRHQDAP"
     gene            4897..5394
                     /locus_tag="P5179_00765"
     CDS             4897..5394
                     /locus_tag="P5179_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS93784.1"
                     /translation="MDALTVIKTRRSVRAFADRQVPREVLEDIVDCGRLAATARNEQP
                     WEFIVVTDPAMRAKIADLAEYGKFIAQASACIVVVCKDGRYFLEDGSAASQNILLGAW
                     AHGVGSCWVAGDKKPYAVDVAKVLGVPDGYRVISLIALGYPQAPPLPKPKRALKEVLH
                     WEKWA"
     gene            5547..7064
                     /locus_tag="P5179_00770"
     CDS             5547..7064
                     /locus_tag="P5179_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRS93785.1"
                     /translation="MAEFPVAECWVIEPPDPPHGFHDLVGYLINAGVDISFGPNDTLP
                     NDLPPSCDGIRVIVTCLEDLPRLRRRLRGFVGLDPDTDSLEGVAPHGTMYLHENGRTW
                     LAAYSEKGRFETKRIENPLIVAPDLTINSPAMRARMLARSDRDVHRMLLEGLMQSDRH
                     EWSDVSFGTLWAMIGAFEATGEERYREAAVRFADTQLARWDAGGRKDGGPEISSLAFL
                     RLSEVSGDPRYRKEILAAIPEVPEKSRYRPAFGAVDDFRKQVGTGLLLQAHSFVHPAT
                     RAVLESRAADGGTYSEQVGLELASVLAAARAGGWSHDATPVIAACLAAHRENCRDPET
                     GLYYHGLLGRRGAHKGFQGHGMLWTCFGLQNTLEQWPTDDPAYAEVLRMYGDACDAAA
                     TVQDPETGEFHHILDMPQSPFGRIYTPALAYVFLRGARLGLLSGEFHERGIRAWEAMK
                     CHIFQGGSVGGDAGTPQMKRFEAYLLQPVTYNYRNNRTRCFWQAHAANEVLRTIA"
     gene            7074..7871
                     /locus_tag="P5179_00775"
     CDS             7074..7871
                     /locus_tag="P5179_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93786.1"
                     /translation="MPHVVLVMPDRALRLLWEHAFSVAGWRTDPADTAIDALAAAFNA
                     EADAIVVALTDAISAEIGRETLGELEKDSELSVIPRFALAVDGPGADVPGVRVFPQPC
                     DPFWLICEADAALGRETPRGLGRRPFSESDTDVMVHAWWWTLAAGDLVQRVMRFDADG
                     ITGEALAVKVAELLGLRADVQIEIGHGDPPQGARNFRYYGEIGGVENGIVILNDSAAS
                     ETWWRVTLPREGVSRGVAEAIAAVARALRIILRRDAAAGGCISQTPE"
     gene            complement(7854..9824)
                     /locus_tag="P5179_00780"
     CDS             complement(7854..9824)
                     /locus_tag="P5179_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93787.1"
                     /translation="MAQSLSSPSESNESAAPGRANLVVTALADPVRAKQVLAGRAAVD
                     PVDGREKFWITNMNEQYGGELIAIDFEGDTAEVFRWPAGQGSWCVLPLPRERLAISTY
                     YDGKFLVFDMRRREFTRVVSFPGESYVWEMATGSDGRVYGGTYPGAKLGCFDPETGEF
                     EDCGSPVAGTENQYLRNVATAPGGDIICTFGSASQSVRVYRIAEKRFEFLVPDEPDFF
                     AKPIVSLRGHLFVQHPERGLLAFRGPQLEPVERLPLPDCPVGSGWKGIARYSTEERVH
                     LTAERQTWEWLPATASLTLVTDLTLRGCRVFDVAQDGRLLGIRGQDYFALPAGSPRLE
                     LRRIPAESAGRPFHFLTADPPDSPRPHRIWGGPSYGQTVASYEIETGKVWNSGAVIDS
                     SGEVYGAVSVGGKLYTASYSGADFAVYDPARPWDQWNNVNPRHIGSISDQHQCRPTGR
                     MRLGPDGNLYSGWQAAYGEYGGALARLNPRTEEITVWRNPLGDEPVISLAVGGRFAYL
                     GTDQTANGLPRREGKGQFGVWSLAEERVVFQQRMETMNAVRCIGAMRTKELAFFPERD
                     ELHVFDGESLQFVEKIPAPVPDSPGWCEDVLLHPSGYLLYARGPFLVRVAPDLRVEIL
                     GPLEKPVNHMTFGPDGLLYFSSGTTLYSLRGL"
     gene            complement(9919..11835)
                     /locus_tag="P5179_00785"
     CDS             complement(9919..11835)
                     /locus_tag="P5179_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93788.1"
                     /translation="MNPAVTALADPVRAKQVLSGRVITDPVDGREKFWITNMNEQSGG
                     ELISVDFDRDTAEVFGWPAGHGSWCVLPLPKDRLAISTYYDGKFLLFDMVKRQFTYVI
                     DFPGESYIWTMAEGSDGRVYGGTYSGAKLGCFGPDSGEFEDCGSPVAGTGNLYLRTVT
                     TTPGGDIACTFGYERSSTQVYRVAKRRFEYLLPENPDFTGSPLASLRGHLFVSDGERG
                     LMAFQGPELKPVDRLPLPKCPGGKAWTGVARFSTDERVYLTVGQQLWLWLPETGRLAL
                     VHDVDLRGGNIFDVAADGRLLGIRGQDYFVAQPGTRTIALRRIPAESKGRPFHFMAAD
                     LPGSPEPHRIWGGPPFGQTVACYEIGSGKVINTGAVVDSGGEVYGGVALNGKIYTASY
                     AGADFAVYDPAQPWNQWEGINPRHLGSIKTHRVCRPTGRMYLAPDGNLYSGWQANYGE
                     YGGALARLNPATEEITVWRNPLGDEPIISLAVDDTYAYMGTDHSANGLPRREGKGQFG
                     VWSLKDERVVFQQRMETMEGVGHIGVISNPRIAMFPEGDALHVFNAEKMEFERDLAAA
                     VPDSPGWCEDVLRSFDGTWVLYARGNHLIKVHQDLSVEVLPPIEHAINHMVFGPDGNL
                     YFSSGTTLYVLEGL"
     gene            12019..13281
                     /locus_tag="P5179_00790"
     CDS             12019..13281
                     /locus_tag="P5179_00790"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS93789.1"
                     /translation="MAEKLAIHGGEKVRTKGFPTVGDASGRMIGEEEKRLVLEVLDSG
                     RLNKNNGTKVAEFEKKFAEMYGVQYAVASTSGTSALHLAVAALDLEPGDEIITTTITD
                     MGSLIGILMQNLIPMFADVDPRTGNITADTIEKVISPRTRAIMPIHLFGQPCDMDPIL
                     DLAAKRGLRVIEDASQAHWAEYKGRRIGTMGDLGCFSFQQSKQMTTGDGGMTITNDPA
                     LAERARLFGDKAWPRNSGRGHAFLGMNYRMTELQGAVGLAQLGKLETILQRRRTTAEL
                     LDSQLAGVPGIIRPFRYDGVVHSWWIYSFTIDEGVFGISPKEFASALQAEGIPFGCGY
                     IPNPMFDYPVIQERKTYGTSGIPWTLPQARPGIRYSDDDAPNTIWFLSHVLIMSWNEG
                     ITESDAMDLAKGIKKVAAWFKENPGSKA"
     gene            complement(13428..14513)
                     /gene="asd"
                     /locus_tag="P5179_00795"
     CDS             complement(13428..14513)
                     /gene="asd"
                     /locus_tag="P5179_00795"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRS93790.1"
                     /translation="MSRVLNVAVVGATGVAGQQALVSLDNHPWFKVVALAASHRSAGK
                     TYREAITTASGQLRWACQEPVPAWADDLLVQNAEELSLDGIDIVFNATDGDSAKILEP
                     RYAKTTPVVSTTSAFRYEDDVPIFIPGVNLDHAPLIRVQQKNRGWKGFVTPIPNCTTT
                     GLAMSLAPLHRTFGVSGVVMTSLQAISGAGRSPGVLSLDILDNVIPYIPKEEEKVERE
                     TQKILGTLDDSVIRAADFPVSATCMRVNVRDGHTEAAYVGLKKRASVDEVADAMRAFG
                     KDLVALNLPSGPRQPITVVSDPYHPQPRADRDLDGGMTTVVGRIREDRLLPNGIKYVL
                     LSHNTKMGAAKGATLVAEYLYKAGYLS"
     assembly_gap    14634..14733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14930..16645
                     /locus_tag="P5179_00800"
     CDS             14930..16645
                     /locus_tag="P5179_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS93791.1"
                     /translation="MKRRVLIIGGVAAGASAATKARRTDETAEIVMFERGDYVSFANC
                     GLPYYVAREIVERENLLVVTPEQFRIRHEIDVRLAHEVTRINPREKTIEVRDLQAGTS
                     RTERYDSLVLTPGASAIIPPIPMVNASNVFTMRTIPDMDAVHEYIERMHPQDATVVGA
                     GYIGVEMVEALHRRGLQVTLVEKLNQVLPPLDEDMAFMVADHIRAEGVDLHLGDGVRS
                     FRGNDNRVGEVELESGAVIRADIVIMSIGVRPDTKLARDAGLQLGVSGAIAVDSRMQT
                     SDPHIWAAGDAVESVHRVTGKPCWVALAGPASKQGRVAGANAVGGTMEAPPVLGTSIV
                     RFNEIVAAVTGLNERSARTAGYDVESSVIHANQHAGYYPWAELLTIKTVYERQTGRLL
                     GAQVIGNEGVDKRIDVFATALTANMRVADLTELDLAYSPPFGSARDPVNIAGFVSQNQ
                     LSDAVSPISSTDLHALLGQSPEVQLVDVRTPAEFREKHIRGARLIPVDELRKRLGEID
                     KTRPVVVYCRVGLRGYLASRILAQNGYSVRNLSGGIRIWRFETEADISDAEAKSKYGN
                     DVSRA"
     gene            complement(16686..17279)
                     /locus_tag="P5179_00805"
     CDS             complement(16686..17279)
                     /locus_tag="P5179_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS93792.1"
                     /translation="MNPARTGFFDEIAPQWDEKSGPEAIERVRAWCANLPLPSDGVVV
                     DLGCGTGISSQAIVEGTNRRVVAVDLSLEMLRNGRSKRDADSICWVCSNAMELPLADS
                     VADGVLALHVLPHLERRGIAFSEWRRVLKPGGSLFITHLMSRARVNEIHRSGPEAIHD
                     DILPPARELAGELVSHGFCVLETEDSHERYLLHCARL"
     gene            complement(17276..17800)
                     /locus_tag="P5179_00810"
     CDS             complement(17276..17800)
                     /locus_tag="P5179_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93793.1"
                     /translation="MNRSVATITRTGLVVALGLGFPWAIHMAGAGQIGKFLLPMFLPI
                     VGGAFFLPFGAAVFAGAATPFLSSILTGMPPLAPPIGPIMMVELAAMAGMVVLVYQKL
                     RANVWVTIASAVIVDRLILAFLAAGLGKMLKLPPAMITWATLVVGIPGMVLLFVVLPP
                     VVARLKAYSIREQT"
     gene            complement(17797..19650)
                     /locus_tag="P5179_00815"
     CDS             complement(17797..19650)
                     /locus_tag="P5179_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS93794.1"
                     /translation="MSQHAPVPSKGAAIILLFLSGFCARMAAADTTYVMDPLVVTATR
                     VETERRNLPGSVTVIQRADLLATGATGVLDGIAASTPGVFVTQRGPAGFGLGSGAAGK
                     LTIRGIGGNPNSQVLVLVDGRPDFTGLFGHPLPDAYALSEVSRVEVVRGPSSAVYGTN
                     AMGGVINIITQRKRTVGNSLALRAAAGPWNTYDGEITALGKFASGLDYRVTSGYTTTD
                     GHRKKADFERAHFGAALGYSSGPWDVRGQLTTTPFETHDPGPVSPAITNDRWIDVTRS
                     SASVTGTYRRDALSSTLMVHRSWGTHKFYDGWHSNDYINGVIAQSSYSFVHGLVVTAG
                     ADAKQYGGDAVNDTTGYDYGTHRVTEIAPFATIQAPIGNLANLTASARAQHHDRYGWF
                     LAPEAGAAFHVLPRLTVRTNVSRGFRSPDLRALFLFPASRDTLKPEKTFNTEAGVSWR
                     ATDWLTSDITVFRTKASAIIENAPAPPFPPPFVNRGEFSSRGVEFETRWWISRIHGRL
                     SASLQDLGNQTAGSAETVVGGTVVLPAAQGRMALDCQWVHNLYGADRHQQKLNDYTVL
                     NATLAYPVWRGIEAQIVMRNLLDTKYETMPGYPMPGFHALFGVNAQIGSAE"
     gene            complement(19773..22037)
                     /locus_tag="P5179_00820"
     CDS             complement(19773..22037)
                     /locus_tag="P5179_00820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS93795.1"
                     /translation="MPDAFIQSPDPVPFERLSNWQYHPGDMLPEEVWSTHDSWHEGGR
                     TLIWDDDVRIVWFREEWTVPRAWAGRALVLSIEVRNTFSLYVDEQAVTAPHLLSRSAG
                     PNRTFRLAIRGDRVRRPGMIMRAEIHSFPPEYAAWLDAKGALRAMAPGRGLLLEGWRV
                     HREEPGTDFADPLLDHSSWQARKLGEQWRERDSCYWYRMTVEVPEQIESFPVEGSRLH
                     LSASFNHSGSIWVDGEKKAEYARNFGNAVVTNHAKVGSRHVVALRVPTPWTGWVRDTY
                     LAPEGLLAARKAYDSLSGMVTFWDRFFQDRPEPQLITAVCVVLTDLAEKRVSGPALTT
                     ALTEASDELDRLVKTYSDDAPFLALPYLQLPSPGGIIVRAESPVARPAYLALRDCDGE
                     TILIPDEGSTRFHRFVVHGLQPDTPYSYTVGAGNVTTEINHFRTAPLTPRPITVFMWG
                     DSHYGPSVLEGLAERIEEVQPDLLITAGDMVGDGVNENEWRDYVFHPLRHVAAKYPLH
                     FPVGNHDHGSWRHRGHGDNPFLNERWEPSGTGWGNTPYGYSFDYAGVHFVFVDPLYGC
                     TGNETFADLNKGTAQYEWLERDLSASRGAQWTILLVHEPPFCETWEGGYYDGQPELRE
                     HLVPLMEKHGVDLCVSGHAHTYERGIPHPPFDPANGEGNTVAYLITGGGGSLLDNRKY
                     REWPQIDIPPHRVEQTENFLKNDIGEYYRYHYCVLRIEPQRLECTAYWIRLDGSVVDT
                     LDWFVLRKGIPARS"
     gene            22239..23567
                     /locus_tag="P5179_00825"
     CDS             22239..23567
                     /locus_tag="P5179_00825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRS93796.1"
                     /translation="MKLTVVGTGYVGLVSGACFAEMGNDVWCVDSNCMKIQSLLEGRI
                     PIYEPGLEDLVKSNLEAKRLHFTTRLEEGLATSLFVFIAVGTPPDDDGAADVGNVLEV
                     AREVGRKLERYAIIVIKSTAPVGTADKVRDAVSEELRGRGRTDIEFDVVSNPEFLKEG
                     AAIEDVMKPDRIIVGTDNVRTAELMKELYAPFVRSGHPILTMDVRSAELSKYAANAML
                     ATRISFMNELASLCERMGADIEQVRAGIGSDRRIGNAFLYAGLGYGGSCFPKDVKALV
                     HTMKDAGVAPLILEAVEEVNRRQKISFVGKVRSHLGSLRGKRIAVWGLAFKPQTDDMR
                     EAPSLAILSEILADGAEVRAYDPVAVEAARLLLPHDHLTYARDAYDALEGAHALILVT
                     EWHLFRNPDFERMKSLMDEPVIFDGRNQYDPQILQNQGFRYFCVGRSQSA"
BASE COUNT         4771 a         6907 c         7207 g         4598 t
ORIGIN      
        1 cagctcgcgg aagaggccag cgccgttcac cccgccgccg aggataagga ccggaaccct
       61 ccgagaagcg tcttccgcga gactcacgct ggtaagaaac cggttcccaa tcggctgcca
      121 ctcgcggtgg gtggcgaccc aggcgttgct ctcgggacca cctatcgcca cgatctgatc
      181 ggtctggagg acgtcgtcgt tctggttttc cgtgtcgagg tacggcgtcg cggtcactcc
      241 ctgttcggca agggcgacga cgatgaggtc ggctgcttca cgcgcaacgg gatcgtccgg
      301 atatattacc cggaccatcc caagcggaat tccgacgcgg gaaccatccc cggactctcc
      361 aacgagggag acgctcgggg aggtggcaat ccatttctcc gcgctgaaca tggcgcatcc
      421 ggagtaaatg cgcccctgat gcgttctgaa atccagaaat gaacgcgatc ggcggataac
      481 ccgctggccg aatcggtctg accagactgg agccaaccct ctgagcccca caggaaacag
      541 aactccgaac atgtcccggt cgaccggctc cgggtttttg ccttcgttgg atcctcccca
      601 cgggcgggtg ttcgtgtcgg agcccaccag ccggcttccc gcgtaaccgc tgaggatgac
      661 agaacactca atccgtgggg tgtggtcaaa aagctccaga gtccgaacaa gatgatcgca
      721 ttcggggaag ggctgacgta ttaccacgcg ccatccgagc gcggttcttt ccgcgcgcac
      781 gtgtgccctg atggaggatg cgaacacccg gtctcctgtg gtgcaaaact cccatgaggg
      841 ttcaacccct gggccctctt tgagaagcac gaaatcgttg aggggatacg gcgatgctcc
      901 ggaggcgatt tcccgaccgc ttttgcgctc gatgaggctg acgatgccac ccccgcggag
      961 cgggtccacc gtgatgcggg cgaagggggt ttccagaacc aaaggcgcgt catcggactc
     1021 gatgacttcc tcccgggggg agtcttctgc atccccgtcg gaaagccagt atgtcgaata
     1081 acccagtcct ggaacgtgtt ccgcaaggaa ccgggcgctc tttcctgttt cccgctgaac
     1141 gactgcgacc ggcgtctgaa ccccggtgtc gttcaacgct cgtacacgcg tgccggtgaa
     1201 cgcatgggga agcggcactt ccacgacgtc ggttctggtg tgaccgaggc tgttgaacac
     1261 aacaactgga gtgccggtgc gaccatcagt ggtgatctct tctgcgagat tcgacgtggc
     1321 cgttctgcgc gcagaaattc cctcttccag cgcttcacga aggtgcgtga gcaggtcgag
     1381 atacgactgg tcgttcgacg tacccgtgat ggagtcgtga tgctgggaaa acaaaagctg
     1441 ccgccacgcg tggtcgattt ctgcttcggg gtaaggatgt ccgtagagcg tggcgatccc
     1501 ggaccaggtc tcagcgtctc caagagcctc ctcggcgatg cgcatcgcga tcttgagctc
     1561 aatacggctg agtgcagttc ccacgtggta ctggctgtgc tctcgtgcgg tcaaaggggg
     1621 atggaactcc ccggccttga tgcgggcgga gacttcttca aaaaacgctg atggctgact
     1681 cataaggaca cgatgatccc gaagagcgct gcctttcccg ctgatccagc cgggcgggga
     1741 gacgaaatcg cgcccgcgga aaagcagatt cggtatcggc gcgtcccccg gataggcgtc
     1801 tccggccttc aggacaccac cgatctggtc ggaagcgccc cagtccaccc cgccgtacga
     1861 cgtgacgcgg gtgaggatgg aggagccgtc gggcgccagc cattggaagc acggatcagg
     1921 tgcgcccggt ttggggctcc ctttcgtcca tgtggtggcg atgaacccgg ccttgcgaag
     1981 aatctgcggc aactggcgca catgcccgaa cacgtcgtgc ggcatgtaga tatcgccgcg
     2041 gccgccgagg tagccgacct gaaaccactg gccgtaaaga atgtttctta cgagggcttc
     2101 gccgccgacg ctgttttcgt ttggttcgct gtacgaaccc gacgtctcca cacgtccttc
     2161 tgccatcaaa cggagcagga actcacgatc ctccgcgtag aggttccagt agccgtgcag
     2221 atagtcggtt tcggaaagaa tgcaacggaa ttccgggtct tcccggcaca tgctcacgta
     2281 ttgcgcggca agctcgacgg attcctcgat gtgcgtggcc tgcgggcagg taaagatcgg
     2341 gtcgcagtgg aaatgcatac agaggtgggt aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nngggcgcgc tgtccggtgg ggcaacctga ctgcatagcg caaccgccac
     2521 tcgcactcaa acggtagtcc gcgcactccg ctgtgcggca ttgcacgtct ctcggcagat
     2581 gcgggggcgt ctcgatacaa cgaacgccgc cgcgttcggc actcgacgac cgtgctgcgc
     2641 gggcgcaacg atctgcggcg ttggtacgtg gaatcggcag gggtatacta gtgaaaagct
     2701 gaccttgcgg ggtgtctatc cgaaaaatcc ggtcagatag agaacgagta ccaatacgcc
     2761 gaggaacgtg agcgttgtgc cgatgaagtc aagaatcctc tcgcgtgccg ggtaccagtt
     2821 tctgggtgcg ctcatggggt gtccgttggt gtgtccattg actgatattc aatgtctcag
     2881 ggttgatgtt cagtcttcca agcaaaagat acctttcgcg caacgccagg gatgtgacgt
     2941 acagcacacg aattcgcagg agacctccag atggctttga tgatcgattt cgatacgcac
     3001 aacgcggaag tggaagcagt ctggaaggca taccgcgagc gcaaaccgac gcgcgtgccg
     3061 atggttttcg gcatcaaccc gcgcctgacg atgttcgacc acccgtccaa cccccgtggg
     3121 atcacctttg agcaatactc cagcgatccc caactgatga tggagcgcca gatcgagcac
     3181 caggcgtggg tgcggacgaa tatcctccag gacgccccga tgggtctgcc gaaggacggc
     3241 tggagcctct gggtggattt ccagaactac tatgaagccg cgtggtacgg ggcgcgcatt
     3301 cggtactggc cgggtgaagt gcccgacacg gagcccttgc tggaagatga tgcgtcgaaa
     3361 tggtcgctga tcgaaaaggg tatgcccgac cccttcactc atggcagcgc gcagcgcatg
     3421 tgggattatt acgagtactt ctgccgcaaa aaggaggagg ggtggactta cctcgggttg
     3481 cccatcgcga cggtgacatg gcctgccggt ggcaccgacg gtccgttgac cctctgctgc
     3541 aacctgcggg gggccagcag gttcattccc gacatgatag aggatcccga gtacgcccac
     3601 gccatgctca actacatcac cgagacgagc gttgctcgaa tcaaagcgta ccgcgaacgc
     3661 atgggcgccg aactgaagcc tgaacgtgga ggtctcgcgg acgattcggt catgctcatc
     3721 agcacgccga tgtaccggga gatgatcctg ccctatcatc gccgcatatt cgaggaactg
     3781 gctggaaaag gaccgcacag tatccatctt tgtggcgatg cgacgcggca tttcaaaacc
     3841 attcgagacg aactcaacgt gtggagcttc gacaccggct atcccgtgga tttcggctgg
     3901 ttgagaaggg agctcgggcc ggaggtcgaa atctacggcg gtcccagcgt cccgttcctg
     3961 cgagccgcga caccggacgc ggtccgtgcc gaggtcacac gaatcctcgg cagcgggatc
     4021 atggaaggcg gaaggttcgt tctccgcgag gggaacaacc ttgctccggg ggtacccatg
     4081 gagaacctcc gggcgatgtg ggacgcggtg cacgagttcg gaacgtacgc gtgaggaagg
     4141 attggggtgt gtataagctt atcgcctgcg aggttgccag accggagttc gaccacgtca
     4201 tctcgatgtc gcggaagtcg gtttccgtgg aataccttga gcaggggcat cacaatgacg
     4261 tagtgcgagg caggaaagcc attcaggagc gtatagacgc cgcaagcgaa ggggaatggg
     4321 aagccttgct tctagggtac gctctgtgca acaacatgct ggtggggttg caggcgaggg
     4381 tcaagccgct tgttatcgcg cgcgcccacg actgcattac ctggttcctc gggtcgaaag
     4441 agcggtatgc gagcacattt gaagaagctc cggggacata ttactacacg gcgggctggt
     4501 tgaaagtggt acgcagtcac gatcaggcgg gccagtccgc ctcggcgtcg ggcgtgctgg
     4561 tggaaggtga gtacgccagg ctcgtggaac aatatgggga agacaacgcc gcgtatctct
     4621 gggaaatgtc aaacgcatgg atcaagaact accggtacgg agcctatatc gccgtcccgg
     4681 gcatagacca tgacgggcac cggaaacgag tgcaagagat atgcgcgcgc aattcctggg
     4741 aatacaagga gatagaaggg gatctcgggc ttctgcagcg atgggtggac ggcgagtggg
     4801 acgccaagga tttcctgatc gtcaaaccgg gtgacactat cgctccgacc ctggatgcac
     4861 agatcatttc agtgggcaga aaagaaggag gtcagaatgg acgcattgac cgtcatcaag
     4921 acgcgccgta gcgtgcgcgc gttcgccgac aggcaggttc cccgcgaggt actggaagat
     4981 attgtggatt gcggcagact ggcggcaacg gcgcgcaacg agcaaccctg ggagttcatc
     5041 gtcgtgacag atcccgccat gcgcgcgaag atcgcggacc ttgcggagta cgggaaattc
     5101 atcgcacaag cctcggcgtg tatcgtcgtc gtgtgcaaag acggccggta tttcctcgag
     5161 gacggctcgg cggcgtctca gaacatcctg cttggcgcgt gggcgcacgg agtcggttcc
     5221 tgctgggtgg cgggggacaa gaaaccgtac gctgtcgatg tcgcaaaggt gctgggtgtt
     5281 ccagatgggt accgagtgat ttcgcttatt gcgcttgggt accctcaggc gccgcccttg
     5341 cccaaaccaa aacgcgcgct gaaagaggtt ttgcactggg aaaaatgggc gtgaacacgc
     5401 cgaatctgtg atcccggaaa agagcgggaa gcaaccttcc tgcgtggcca gggcttatct
     5461 ggcgaacttt ggaggggctc ggttccagaa aggacagacg acgttgcgta tccggacatt
     5521 gccgaacgcg agtgaggagg ggctccgtgg ctgagttccc ggtcgcagaa tgctgggtca
     5581 tcgaacctcc cgatcctccg cacgggtttc atgacctcgt cggttatctg atcaacgccg
     5641 gagtggatat ttccttcggc ccgaacgaca cgctgccgaa cgatctcccg ccctcctgcg
     5701 acggaatccg cgtaattgtc acatgcctgg aggatctgcc acgcctgcga cgccgcctgc
     5761 gaggattcgt cggactcgac ccggacacgg attcgctgga aggcgtcgcc ccgcacggaa
     5821 ccatgtatct gcacgaaaac ggtcgcacgt ggctggcagc ctactccgaa aaagggcggt
     5881 tcgaaaccaa acgaatagag aatcccctca tcgtcgcccc cgacctcacg ataaactccc
     5941 cggcgatgcg tgcgaggatg ctggcccgct ccgaccgcga cgttcaccga atgctgctgg
     6001 agggcctcat gcaatccgac cggcacgagt ggtcggacgt gagtttcgga acgctctggg
     6061 ctatgatcgg ggcgtttgaa gcgacggggg aggaacggta ccgagaagcc gccgtccgtt
     6121 tcgcggacac gcaacttgcg cggtgggatg ccggcgggag gaaagacggc gggccggaga
     6181 tttcatccct cgcgttcctg cgattgtcgg aggtatcggg cgacccacgg tatcgcaagg
     6241 aaatcctcgc ggctatccct gaagtaccgg agaagtcgcg ttatcgcccg gccttcggcg
     6301 cagtggacga tttccggaaa caggtcggca ccggcctgct gcttcaggcg cacagtttcg
     6361 ttcaccccgc gacgcgtgcg gttctcgaat cccgcgcggc ggacggcgga acgtacagcg
     6421 aacaggtagg gctggagttg gcttccgttc tggccgccgc aagggcgggg ggatggagcc
     6481 acgacgcgac tccggttatt gcggcgtgtc tggcggctca ccgcgaaaac tgtcgcgacc
     6541 cggaaaccgg gctttattac cacggcctgc ttggccggag gggcgcacac aaaggcttcc
     6601 agggacacgg gatgctgtgg acctgtttcg ggctgcagaa cacactggag cagtggccga
     6661 ctgatgaccc ggcgtacgcg gaagttctcc gtatgtacgg ggatgcatgc gacgcggctg
     6721 ccaccgttca ggatccggaa accggtgagt ttcaccatat cctcgacatg ccgcagtcgc
     6781 ctttcggccg aatctacacc cctgcgctgg catacgtgtt tctgcgcggg gcgcggctgg
     6841 gattgctctc cggcgagttc cacgagcgcg gaattcgcgc gtgggaagcc atgaaatgcc
     6901 acatctttca ggggggcagc gttggcggcg atgccgggac accacagatg aaacggtttg
     6961 aggcctacct gttgcagccg gtgacgtaca actaccggaa caaccgcacc cggtgtttct
     7021 ggcaggcgca cgcggccaac gaggtcctga gaactatcgc gtaggagtgc tcaatgcccc
     7081 atgtggtgct ggtgatgccg gatcgcgcgc tccgcttgct gtgggaacac gcgttttccg
     7141 ttgcagggtg gcgtaccgat ccggccgata ccgcgataga tgcactcgcc gcagcgttca
     7201 acgccgaggc cgatgcgatc gtggtggcac tgacggatgc aatatcggcg gaaatcgggc
     7261 gggagacgct gggggaactc gagaaggatt cggagctgag cgtcattccg cgatttgctc
     7321 tggcggtcga cggcccgggg gcggacgttc ccggtgtgcg cgtgtttcca caaccctgtg
     7381 acccgttctg gctcatctgc gaggctgacg cggcattggg acgggagacg ccacggggtc
     7441 ttggcaggcg tccgttcagc gaaagcgata ccgacgtcat ggttcacgcc tggtggtgga
     7501 cactcgccgc gggtgacctc gtacaacggg tgatgagatt cgatgcggac ggcataacgg
     7561 gtgaggcgct tgcggtgaag gtcgcggagc ttttgggcct tcgcgccgac gttcagatcg
     7621 aaatcgggca cggcgatccg ccgcaggggg cgcgaaactt ccggtattat ggggaaatag
     7681 gcggggtgga aaacgggatc gtaatattga acgattccgc tgcctcggag acctggtggc
     7741 gggtgacgct tccccgggag ggagtgagca ggggagtggc ggaagcgatt gccgcggtcg
     7801 cgcgcgcgct gcgaatcatt ctccggcggg atgcagccgc cgggggctgc atctcacaga
     7861 cccctgagtg agtacaacgt ggtgccgctg gaaaaataca ggagcccgtc aggcccgaag
     7921 gtcatgtgat tgaccggttt ttccagcgga ccgaggattt ctacgcgaag atcaggggca
     7981 acccgcacga gaaaaggtcc tcgcgcgtac agcaggtacc ctgagggatg gagcaacacg
     8041 tcctcgcacc atcccggcga gtcaggcacc ggcgcgggga ttttttccac gaactgaaga
     8101 gattccccgt caaacacgtg gagttcgtcc ctctcgggga agaacgccag ttcctttgtc
     8161 cgcatcgcgc cgatgcaccg gaccgcattc atcgtttcca ttctctgctg gaaaaccacg
     8221 cgctcctcgg caaggctcca cacgccgaac tgccctttcc cttctcgccg cggcaaaccg
     8281 tttgcggtct gatcggttcc gaggtacgca aagcggccgc cgacggcaag cgagatgacg
     8341 ggttcgtcgc cgaggggatt ccgccagacg gtgatttcct cggttcgcgg gttcaaacga
     8401 gccagagccc cgccatattc cccgtatgcc gcctgccatc cggagtaaag attcccgtcg
     8461 gggccgagcc gcattcgtcc tgtcggccgg cactgatgct ggtccgagat ggagccgata
     8521 tgccgcgggt tcacgttgtt ccattgatcc cacgggcgtg cgggatcgta cacggcgaag
     8581 tccgcgccgg aatacgaagc agtgtacagt tttccaccga cagaaaccgc gccgtacact
     8641 tctccgcttg agtcaatcac ggccccggaa ttccagactt tcccggtctc gatctcatac
     8701 gaggcgacgg tttgtccgta cgatgggccg ccccagatgc ggtggggacg gggcgaatcg
     8761 ggtgggtccg cagtgaggaa atgaaacggt ctcccggcgc tttcggccgg aattcggcga
     8821 agctccaaac gcgggcttcc ggcgggcaat gcgaagtaat cctgaccgcg gatgcccagc
     8881 aggcgaccat cctgtgctac gtcgaaaacc cggcaaccgc gcagtgtcag gtccgtcacg
     8941 agagtcagag aagcggttgc cggaagccat tcccacgtct gccgttcggc ggtaaggtga
     9001 acacgttctt ccgtagaata ccgtgcaatc cctttccacc cgcttcccac ggggcaatcg
     9061 ggaagtggga gccgttccac gggctccagt tgcggacctc tgaatgccag aaggccgcgc
     9121 tcggggtgtt gcacgaaaag gtggccacgg aggctgacga tcggtttggc gaagaaatcc
     9181 ggctcgtccg gaacgaggaa ctcaaaccgt ttttctgcga ttcgatagac ccgaacggat
     9241 tgacttgccg acccgaacgt gcagataata tctcctcccg gcgccgtcgc gacgttgcgc
     9301 agatactggt tttccgttcc ggcaaccggg ctcccgcaat cttcgaactc tcccgtctcc
     9361 gggtcaaaac atcccagttt tgcgcccggg taggttccgc cgtacacgcg gccgtctgat
     9421 cctgtggcca tttcccaaac gtagctttca ccggggaatg acacaacgcg cgtgaactcc
     9481 cgccttcgca tgtcgaacac gaggaattta ccgtcatagt acgtgcttat cgcaagccgt
     9541 tcgcgtggca acgggagaac acaccacgat ccttgcccgg caggccaccg gaagacttcg
     9601 gcggtgtccc cttcgaagtc gatcgcaatc agttcaccgc catactgctc gttcatgttg
     9661 gtgatccaga atttctcgcg accatccacg gggtcaaccg ctgcgcggcc cgcgagcacc
     9721 tgctttgccc ggacggggtc tgcgagagcc gtcacgacca ggttggcgcg gccgggcgct
     9781 gccgattcat tgctctcgga cggtgaggaa aggctctgcg ccatgctgtg cttccttgca
     9841 agcagacgtg cgggaagcag gagagaaccg attcctgttc ccgaccatcg aagaaactcc
     9901 cggcggttca ttatcacctc acagcccttc caggacgtac agcgttgttc cgctggagaa
     9961 gtacagattt ccgtccgggc cgaaaaccat gtgatttatt gcgtgttcaa tgggtggcag
    10021 aacctccacg gaaagatctt gatgaacctt gatgaggtgg ttgcctcgtg catagagcac
    10081 ccacgtgcca tcgaacgacc tcagaacgtc ctcacaccag ccgggggaat cgggaaccgc
    10141 cgccgcgagg tcgcgttcga attccatttt ttccgcattg aatacgtgta atgcgtctcc
    10201 ctcggggaac atcgcgattc gcggattgga tatcacccct atgtgaccga ctccttccat
    10261 cgtctccatg cgctgctgga acacgactct ctcatccttg agactccaca ccccgaactg
    10321 accttttcct tcccgtcgcg ggagaccgtt cgcagagtgg tcggttccca tgtaggcgta
    10381 cgtatcgtcc accgcgagcg agatgatcgg ctcgtctccc agcgggtttc gccacactgt
    10441 gatttcctcc gttgccggat tcaatcgggc gagcgcaccc ccgtactcgc cataattggc
    10501 ctgccatccg gaatacaggt tgccatccgg cgccagatac attcgaccgg tgggacgaca
    10561 cacgcgatgg gtcttgatcg accccagatg acgcgggttg attccttccc actgattcca
    10621 gggctgtgcc ggatcgtaca ctgcgaaatc tgccccggcg tacgacgccg tgtagatttt
    10681 gccgttgaga gcaaccccgc cgtacacctc acctccggaa tccacgacag cccccgtatt
    10741 gatcaccttc ccggacccga tttcgtagca ggccacagtc tggccgaagg gggggcctcc
    10801 ccatatccga tgcggctccg gcgaaccggg caggtcggcc gccatgaaat gaaacggccg
    10861 gcctttgctc tctgcgggaa tccgccgcaa tgcgatcgtc cgggttcccg gctgggcgac
    10921 gaagtagtcc tgccctctga ttccgagcaa ccgcccgtcg gctgccacgt cgaatatgtt
    10981 tcctccccgc agatcgacgt catgtacgag tgcgagccgt cctgtctcag ggagccacag
    11041 ccagagctgc tgcccgaccg tgaggtacac ccgttcgtcc gtcgaaaacc gcgcgacgcc
    11101 ggtccacgct tttccgccgg ggcattttgg gagcggcagg cgatccaccg gcttcagttc
    11161 gggaccttga aacgccatca gtccccgttc tccgtcgctc acgaaaagat ggccgcggag
    11221 gctcgcaagc gggcttcccg tgaagtccgg attctcggga agcaggtatt cgaaccgtct
    11281 tttcgccacg cggtacacct gggtcgaact tctttcgtag ccgaaggtgc acgcaatgtc
    11341 tccccccggc gttgtcgtga cagtccgcag gtaaagattg ccggttccgg caacaggcga
    11401 tccgcagtct tcaaattccc cggaatcggg gccaaaacac cccagttttg ctcctgagta
    11461 ggttcctccg tacacacgtc catcggagcc ttcggccatg gtccatatgt agctttctcc
    11521 ggggaaatct atcacgtacg tgaattgccg cttgaccatg tcgaacagga ggaacttgcc
    11581 gtcgtaatac gtgctgatgg cgagacgatc ctttggcagc ggcagaacac accatgatcc
    11641 atgacctgcc ggccacccga acacctcggc ggtgtccctg tcgaaatcca ccgatatcag
    11701 ttctccgccg ctctgttcgt tcatgttggt gatccagaat ttctcccgcc catccaccgg
    11761 gtcggtaatc acacgccctg agaggacttg ttttgcgcgg acaggatcgg caagggcggt
    11821 tacggcaggg ttcatggaaa tctcctctga cgggttctat gcagcgtctg gaagcacaac
    11881 ctcgaatgtc atcaagtacg ccatccgcgg gaaagggggc aagccgggca cgttgagtct
    11941 gacctcatcg ccggctaaat tcgcttcagg gacatgctga cgcaacgctc cttttgaacc
    12001 gttggagaaa ggtgtaccat ggctgaaaaa ctcgcgatac acgggggaga aaaggtacgg
    12061 acgaagggct ttccgactgt cggggatgca tccggaagga tgatcgggga ggaggaaaag
    12121 cgtctcgttc tcgaagtcct tgattccggc agattgaaca agaacaacgg aaccaaagtc
    12181 gctgaattcg agaaaaagtt cgccgaaatg tacggggttc agtacgcagt cgcttcaacg
    12241 tcgggcacct ccgcgcttca cctcgcggtg gccgcgctgg atctggaacc cggcgatgag
    12301 ataattacga cgacaatcac ggacatgggg tctctcatcg gtatcctcat gcagaacctc
    12361 atccccatgt tcgccgacgt cgacccgcgc acgggcaaca tcaccgccga cacaatcgag
    12421 aaggtgattt cgccccgaac cagggcgata atgccgattc atctgttcgg ccagccttgc
    12481 gacatggacc cgatacttga ccttgcggcg aagcggggcc tgcgtgtgat tgaagacgct
    12541 tctcaggcac actgggcaga atacaagggc cgcagaatcg gaacgatggg agaccttggc
    12601 tgcttcagtt ttcaacaatc aaagcagatg acgaccgggg atggcggcat gacgatcacc
    12661 aacgatcctg ccctggcgga acgcgcccgg ttgttcggcg acaaggcatg gccgcggaat
    12721 tccggacgcg gtcacgcatt cctcgggatg aactaccgta tgacggaact ccagggcgcg
    12781 gtcgggttgg cgcaactcgg caaactggag accattctgc agcggcgcag gacaaccgcg
    12841 gagctgcttg atagccaact ggcaggcgta cccgggatca tccgcccgtt ccggtacgac
    12901 ggggtagtgc attcgtggtg gatctactcc ttcaccatcg atgaaggagt gttcggcatt
    12961 tctccgaagg agttcgcctc cgcgttgcag gctgaaggca tacccttcgg gtgcgggtac
    13021 atcccgaacc cgatgtttga ctacccggtg atccaggagc ggaaaacgta cggtaccagc
    13081 ggcatcccgt ggacgctgcc gcaggcgcgg ccgggcatcc gctattcgga cgacgatgct
    13141 cccaatacga tctggttcct ttcccacgta ttgatcatga gttggaacga gggaatcacc
    13201 gaaagcgacg cgatggactt ggcgaaaggc atcaaaaagg tcgccgcgtg gttcaaggag
    13261 aaccctggtt ccaaagcctg attcgggacg tgcgcagcga taagtcggag gggagagagg
    13321 tgacactctc cccttttttg ttcgcaggag attcctcggt tcgctcagaa tgacgaaggg
    13381 gcgccgcaac cggcgcccct tcgtcgaatc gtttcccggg tggttcccta cgagagatac
    13441 ccggccttgt aaagatattc cgccaccagc gttgcccctt tggcagctcc catctttgta
    13501 ttgtggctca agagcacgta cttgataccg ttcgggagca ggcgatcctc gcggatgcgg
    13561 cctaccactg tcgtcatgcc accatcaaga tcgcgatccg cacgcggttg cggatgatac
    13621 gggtcactca ccaccgtgat cggctgtctg ggccccgacg gcagattcag cgccaccagg
    13681 tccttgccga atgcacgcat ggcatcggca acctcatcca ccgaggcgcg tttcttgagt
    13741 ccgacgtacg ccgcttctgt gtgcccgtca cgaacgttaa cacgcatgca cgtggccgaa
    13801 acgggaaaat ctgccgcccg gatgacggag tcatccagcg tgccaaggat tttctgggtt
    13861 tcgcgttcga ccttctcttc ctccttcggg atgtacggga tcacgttatc gaggatatca
    13921 agtgagagca ctccagggct ccgccccgcg cccgatattg cctgcaacga agtcatcacc
    13981 acgcctgata ccccgaatgt gcggtgcaat ggcgcgaggg acatcgcaag gccggtagtt
    14041 gtgcagttcg ggattggggt gacaaatccc ttccacccac ggtttttctg ctgcacgcgg
    14101 atcagcggcg cgtggtccag attcaccccg ggtatgaaaa tcggcacatc atcctcgtat
    14161 cggaacgcgc ttgtcgtgct gacgacaggc gtcgtcttcg cgtagcgcgg ttccagaatt
    14221 ttcgcagagt ccccgtccgt cgcgttgaac acgatgtcga tgccatccag cgacaactcc
    14281 tcggcgttct gcacgagcag atcatccgcc cacgccggaa ccggttcctg gcatgcccag
    14341 cggagctgac cggacgcagt ggtgatggct tccctgtatg tctttccggc gctccggtgc
    14401 gacgccgcaa gcgcgaccac tttgaaccat gggtggttat cgagcgaaac aagtgcctgc
    14461 tggccggcaa cgcctgttgc accgaccacc gcgacgttca acacgcgact catgaaacac
    14521 tccttgggtg ccgttggttg cgttcaagac acagaaatac cggcgttgct aagaacagcc
    14581 gccggctcaa gtacaagtgt acgtcatggc ggcctcgttc gccaaggaat acgnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtgt ttcacaaagg gcgccctata
    14761 cacgccatga tgggtgcggg gcctctcggg catatgcgca ggttcgttga ggacagcctt
    14821 gcgaaggtgc ccggtcacat tatcgtcgcc cgttgattcc cgcccgaatc cgagtcgcgg
    14881 cagtgaggat tcctcgcctc accgccgcgg ctcatctctg gagaagcaca tgaaacgccg
    14941 cgtattgatc attggtggag ttgccgcggg tgcgagcgcg gcgacgaaag cgcgccgcac
    15001 agacgaaacc gctgaaatcg taatgtttga gcggggcgat tacgtttcgt ttgcgaactg
    15061 cgggctgcca tactatgtcg cgcgcgaaat cgtggagcgc gagaaccttc tcgtcgtgac
    15121 gcctgagcag ttcaggatac ggcacgaaat tgatgtgcga cttgcccatg aggtaacgcg
    15181 catcaacccc cgggagaaaa cgatcgaggt tcgcgacctg caggcaggca cgtcacgcac
    15241 tgagcgttat gattccctcg tcctgacacc cggcgcaagc gcgataatcc cgccgattcc
    15301 aatggtcaat gcatcgaacg tgttcacgat gcggactatc cccgacatgg atgccgtgca
    15361 cgagtacatc gagcgcatgc atccgcagga cgccaccgtc gtgggggcgg ggtatatcgg
    15421 tgtggagatg gtcgaagctc tgcaccgaag ggggcttcag gttaccctcg tggaaaagct
    15481 gaaccaggtg cttcctcctc tggatgagga catggcgttc atggttgctg accatatccg
    15541 cgctgaggga gtcgatcttc atcttggcga cggcgtgcgc tccttccgtg gaaacgacaa
    15601 cagagtcggc gaggtggaac tcgagagcgg agctgtcatc cgcgccgaca ttgttatcat
    15661 gtctatcgga gttcgtccgg atacgaagct cgcgcgcgac gcgggattgc agctcggggt
    15721 gagtggcgcg atcgcggtgg attcccgcat gcagacgagc gatccgcaca tctgggcggc
    15781 gggagacgcg gttgagagtg tgcaccgcgt tacaggaaaa ccgtgctggg tcgcgctcgc
    15841 cggtccggcg agcaagcagg ggcgggtagc cggggccaac gcagtgggcg gaacaatgga
    15901 agccccgccg gttctcggaa cgtcaatcgt gcggttcaac gagatcgtgg cggcggtgac
    15961 aggattgaac gaaaggtccg cccgcactgc gggttacgat gtggaaagct ctgtaataca
    16021 tgccaaccag cacgccgggt attacccctg ggcggagctc ctcacgataa agaccgtcta
    16081 cgagagacag acagggcgtc tgcttggcgc gcaagtgatc ggcaacgaag gggtggacaa
    16141 gcgcatcgat gtgttcgcca ctgcccttac ggcgaacatg agagttgccg acctgacgga
    16201 actggatctc gcgtactcgc cacccttcgg ctctgcgcgg gatccggtga atatcgcggg
    16261 gttcgtttca cagaaccaac tatcggatgc cgtgagcccg atttccagta cggatttgca
    16321 cgcccttctc ggccaatcgc cggaggttca gctcgttgac gtgcgcactc ccgccgaatt
    16381 tcgagagaaa cacattcgcg gggcgcgcct cattccggtt gacgaactcc gaaaacgcct
    16441 tggcgaaatt gacaaaacca ggccggtggt cgtgtactgc cgggtgggcc tccggggcta
    16501 ccttgcgagt cgaatccttg cgcaaaatgg gtactcggtg agaaacctat cgggaggcat
    16561 tcgaatctgg cggttcgaaa cagaggctga catttcggat gctgaggcga agtcgaaata
    16621 cgggaacgac gtttcccggg cctgagcgcc gcgcaaccgg tccgcgggag gctctcgacc
    16681 gctcttcaga gccgtgcgca gtgaagcagg taccgttcgt gcgagtcctc tgtttcgagg
    16741 acgcagaacc cgtgggagac cagctcccca gccagctccc gcgcgggagg aagtatgtcg
    16801 tcgtggatgg cttcgggccc ggagcggtgg atctcgttca cgcgggcccg ggacatgagg
    16861 tgcgtaataa agagggatcc gcccggcttc aacacacgcc gccactcaga aaaggcgata
    16921 ccccggcgtt ccaggtgagg caacacgtgc agcgcgagca ccccgtctgc aactgaatct
    16981 gccagaggca attccatcgc gttgctgcat acccagcaaa tcgaatctgc atcccgtttt
    17041 gaccgcccgt tccggagcat ctcgagcgag aggtcgaccg caactacgcg tcggttcgtc
    17101 ccctcaacga ttgcctgcga cgagatccct gtcccgcacc cgagatccac aacaacgcca
    17161 tccgagggca ggggcaagtt cgcgcaccat gcccggacac gttcgatagc ctcagggccg
    17221 ctcttctcgt cccactgggg ggcgatttcg tcaaaaaagc ctgtacgggc tgggttcacg
    17281 tctgctcccg gatggagtat gctttgagac gcgccaccac tggtggcagg acgacgaaga
    17341 gaaggaccat tccgggaata cccaccacca gagtagccca ggttatcatc gcgggtggca
    17401 acttgagcat cttcccgagc ccggcggcaa gaaaggccag aatgaggcga tcaacaataa
    17461 cagcggaggc gatggttacc catacgttgg cccgcagttt ttgatacacc agaactacca
    17521 tccccgccat ggcggcgagt tcgaccatca tgatcggtcc gatgggcggc gcgaggggtg
    17581 gcataccggt gagaatcgaa gataggaaag gcgtggcggc accggcgaac accgccgcgc
    17641 cgaaggggag aaagaacgct ccccccacaa tcggcaggaa catgggcagc aggaacttgc
    17701 cgatctggcc cgcgcctgcc atgtgaatcg cccagggaaa gccaagacca agtgcaacga
    17761 cgagcccggt gcgcgtgatt gtggctacgg aacggttcat tctgcggagc cgatctgcgc
    17821 gttcacgccg aaaagtgcgt ggaagccggg catcggatac cccggcatgg tttcgtactt
    17881 cgtgtccaga aggttgcgca tcactatctg ggcttcaatc cctcgccaga ccgggtacgc
    17941 cagcgttgcg ttgagaacgg tgtaatcgtt gagtttctgc tggtgacggt cggcaccgta
    18001 caggttgtgc acccactggc aatccagagc cattctcccc tgtgcggccg gtagcacgac
    18061 cgtacccccc acaaccgttt ccgcactccc cgccgtctga ttgccaagat cctgcaacga
    18121 ggccgaaaga cgaccatgga tgcgcgatat ccaccatcgc gtctcgaatt ccacgcccct
    18181 cgaactgaat tcaccgcggt tcacgaatgg tggaggaaac ggcggcgcgg gggcgttttc
    18241 gatgatcgcg ctcgctttgg tgcgaaacac cgtgatatcg gaagtcagcc agtcggtggc
    18301 gcgccagctg acacctgctt ccgtattgaa cgtcttctcc ggtttcaacg tgtcacgcga
    18361 agcggggaac aggaacagcg ctctcaagtc cggactccgg aagccccggg acacgttcgt
    18421 tcgaactgtg agccgtggca gaacatggaa cgcggcgccg gcctccgggg cgaggaacca
    18481 gccgtaccga tcgtggtgct gtgcgcgtgc ggaggcagta aggttggcaa gattgccaat
    18541 cggtgcctga atcgtagcga atggagctat ttcggtcacg cggtgtgtgc catagtcgta
    18601 acctgttgtg tcgttcaccg cgtcgccgcc gtactgcttc gcgtccgcac ccgccgtcac
    18661 gacgagcccg tgcacgaatg agtagctgct ctgcgctatt actccattga tatagtcgtt
    18721 gctgtgccag ccgtcataga acttgtgcgt tccccaactg cggtgcacca tcaacgtgga
    18781 ggataaggcg tctcttcgat aggtacccgt gacggaagca ctgctgcgtg tcacgtcaat
    18841 ccatcggtca ttggtgatcg ccggggaaac tggccccggg tcgtgcgttt cgaagggcgt
    18901 cgtcgtaagc tgccctcgaa catcccacgg tccactgctg taaccgagcg cagcgccgaa
    18961 atgtgcgcgc tcaaaatcgg ccttctttct gtgtccatcc gtggtggtgt agccgctggt
    19021 gacacggtaa tccaacccgc tggcgaactt gcccagcgcg gtgatctcgc cgtcatacgt
    19081 gttccaggga cccgctgccg cgcgcagtgc aagcgagttg cccacagtgc gtttccgttg
    19141 cgtgatgata ttgatgaccc cgcccatggc gttcgtgccg tacactgctg acgacggtcc
    19201 gcgcactact tccacgcggc tgacttcgct caacgcgtac gcgtcgggta ggggatgccc
    19261 gaagaggccg gtgaaatcag gccgcccgtc caccagaacc agtacctggc tgttagggtt
    19321 gccaccaatt ccacgaattg taagcttgcc tgccgcccct gatccgagtc cgaaaccggc
    19381 cggaccccgc tgggtgacga aaactcccgg ggtgctggcg gcgatgccgt caagaacgcc
    19441 ggtggctccg gtcgccagaa gatccgcccg ttggatgacg gtgacgctgc cgggcaagtt
    19501 gcgtcgttcg gtttccaccc gggtcgcagt aactacgagc ggatccatga cgtatgtcgt
    19561 gtcggccgct gccattcgcg cacagaagcc gctcaggaaa agcagaatga ttgcggcgcc
    19621 cttagagggg accggagcgt gttgagacat gggttcctcc cgagatcgtc agcatttgtc
    19681 agaatcgtgt gacgaatttg aaaatagtaa caccgacaga cgtccacaac ccttccggtg
    19741 acagatcccg gtcctgggac ccgaacgctc ggtcaactcc tcgcgggaat acccttcctc
    19801 agcacgaacc agtccagcgt gtcaaccact gagccatcaa gccgaatcca gtacgccgtg
    19861 cattccagtc gctgcggttc tatgcgcaac acgcagtaat ggtaccggta gtattcaccg
    19921 atgtcgtttt tcaggaagtt ttccgtctgt tcgacccggt gaggcgggat gtcaatctgt
    19981 ggccactcgc ggtacttccg attgtcgagg agggaaccac cgcccccggt gatcaggtac
    20041 gcgaccgtgt ttccctcgcc atttgccggg tcgaaaggcg ggtgcgggat tccgcgttcg
    20101 taggtatgcg catggccgga aacacagagg tccaccccgt gtttctccat gagcgggacg
    20161 agatgctcac ggagctcggg ttgaccgtcg tagtacccgc cttcccatgt ttcgcagaag
    20221 ggaggttcgt gtactagcag tattgtccac tgtgcgccgc gcgaagcgct caaatcccgc
    20281 tcgagccatt catactgggc ggttcctttg ttgaggtccg cgaaggtttc gtttcccgtg
    20341 cagccataca gggggtccac aaagacgaaa tgcacgcccg catagtcgaa gctgtatccg
    20401 tagggtgtgt tgccccagcc agttcccgat ggctcccacc gctcgttcag aaaagggttg
    20461 tcgccgtgcc cgcgatgtcg ccaactaccg tgatcgtgat tgcccacagg gaaatgcaat
    20521 ggatatttcg ctgcaacgtg gcgtagcggg tggaagacgt aatccctcca ctcgttctcg
    20581 ttcacgccat cgcccaccat atcgccggcg gtgatcagca aatcaggttg cacttcttcg
    20641 atgcgctcgg ccaatccttc cagcacgctg ggcccgtagt ggctgtcccc ccacatgaaa
    20701 acggtaatcg ggcgcggtgt cagcggcgcg gtcctgaagt gattgatttc agttgttaca
    20761 ttgccggctc cgacagtgta ggaatacggc gtatcgggct ggaggccgtg taccacaaac
    20821 cggtggaacc gggtggaccc ttcgtccggg atcaggattg tttccccgtc gcaatcgcgc
    20881 agcgcaaggt atgccggccg cgccacaggc gactctgcgc gaactatgat gcctcccggc
    20941 gagggcaact gaagataggg cagcgcaagg aacggcgcgt cgtccgagta cgtctttacc
    21001 agacggtcca attcgtccga cgcttcggtg agagcggtag taagcgccgg tcccgaaact
    21061 cttttttctg ccaggtccgt cagaacaacg cacaccgccg tgatcagctg aggctccgga
    21121 cgatcctgga agaaacgatc ccagaaggtc accattccgg aaaggctgtc gtacgccttg
    21181 cgggcggcaa gaagaccttc cggggcgaga taggtatccc gtacccaccc ggtccatggc
    21241 gttggcacgc gcaacgcaac cacgtggcgt gagccgactt tcgcatggtt cgtgactacg
    21301 gcattgccga agtttcttgc gtattcggcc tttttctcgc catccaccca gatgctgccc
    21361 gaatgattga acgatgcgga gaggtgcagc cgggatcctt ctacggggaa gctctcgatc
    21421 tgttccggta cctcaacggt cattcggtac caatagcagc tatcgcgctc gcgccattgc
    21481 tcaccgagtt ttcgagcttg ccaggatgaa tggtccagga gcggatcggc gaaatcggtt
    21541 cccggttcct cacggtgaac gcgccagcct tccagcagaa ggccgcgccc gggcgccata
    21601 gcccgtaacg cccctttcgc atccagccac gcggcgtact cgggcgggaa ggagtggatt
    21661 tccgctcgca tgatcattcc gggccggcga acgcggtctc cccttattgc gaggcggaat
    21721 gttctgttcg gacctgcgct gcggctgagc agatgcggag cagtaactgc ctgctcgtcc
    21781 acgtacaacg aaaaggtgtt cctgacttcg atggaaagga caagcgccct gcccgcccat
    21841 gcgcgtggaa ccgtccactc ctcgcggaac cacacgatac gcacgtcgtc atcccagatg
    21901 agggtgcggc cgccttcgtg ccagctatca tgtgtgctcc atacttcttc cgggagcatg
    21961 tcgccaggat ggtattgcca gttggaaagg cgctcgaacg gaacagggtc gggggattgt
    22021 atgaacgcgt cgggcatgta atccttacgt ttcaatagag atgggttagt tgcggaatat
    22081 cggacgtgtt agctttccaa atgggcagtt gacccattga gcgtcacgga agcggcctca
    22141 ggatgatccg gggaaacgag gcacataacc ctcggtagcc cgaaggaacc gattccttca
    22201 ttacgaaaag taaacagtcg gcttccaaag gagaactgat gaaactgacg gtcgtgggta
    22261 cagggtacgt cggtctagtg tcgggagcct gtttcgcaga aatgggaaac gacgtctggt
    22321 gtgtcgatag caactgcatg aaaatccaga gtttgctgga agggcggatt cccatttacg
    22381 aaccgggcct cgaggacttg gtcaagagca acctcgaggc gaaacggctc catttcacaa
    22441 cacgtttgga agaaggtctg gccacctcat tgtttgtgtt catagccgtt ggaactcccc
    22501 cggacgacga tggtgccgct gacgtcggca acgtcctcga ggtcgcgcgc gaggtagggc
    22561 gcaaactcga acggtatgcc atcatcgtga tcaaatctac tgctcctgtc ggaacggctg
    22621 acaaggtgcg ggacgcggta tctgaggaac tcagaggccg tggccgaacc gatatcgaat
    22681 ttgatgtcgt ttcaaatccg gagttcctga aggaaggtgc cgcaattgaa gacgtaatga
    22741 aaccggatcg catcatcgtt gggaccgaca acgtcaggac tgcggagttg atgaaggaat
    22801 tgtacgctcc gttcgtgcgg agcggtcacc ccatcctcac catggatgtc cgttccgctg
    22861 agctctccaa atatgccgcc aatgccatgt tggctacccg gataagcttc atgaacgaac
    22921 tcgcgagcct ctgtgaacgg atgggagcgg atatagaaca ggtgagagcc ggcattggtt
    22981 ccgacagacg tatcgggaat gcctttcttt acgcgggact cgggtatggc ggatcatgct
    23041 ttcccaaaga cgtgaaggct ttggtccaca ccatgaagga tgccggcgtg gctccgctga
    23101 tcctcgaggc cgtcgaggag gtcaatcgcc gccagaaaat ctccttcgta ggcaaggtca
    23161 gatcccacct tggctcattg cgcggcaagc gtatcgccgt gtggggcctg gcattcaaac
    23221 cgcaaacaga cgacatgcgt gaagccccgt cccttgccat tctttccgag atactcgcgg
    23281 atggagccga agtaagggct tacgatccgg tggcggttga ggcggcgcgt cttctgttac
    23341 cgcacgacca tcttacgtat gccagagatg cctatgatgc tctcgaaggt gcgcatgcgc
    23401 tgatattggt aaccgaatgg catctgttca ggaacccgga cttcgaaaga atgaaaagcc
    23461 tcatggacga accggtgatt ttcgacgggc gcaaccagta cgatccgcag attctccaaa
    23521 accaggggtt tcgttatttc tgcgtgggaa gaagtcaatc tgcttgaggt gcctgtctgg
    23581 gtccgaaaac cggtagccta ctgaaacagg agcagcgtgt acaccagggt gaagtagatt
    23641 ccgatcccga cgaaaaccac cccggttacc caccttgccc aca
//
LOCUS       DAOTNG010000008        22612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2351_length_22612_cov_5.090083, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000008 DAOTNG010000000
VERSION     DAOTNG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 22612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22612
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_22612_cov_5.090083"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..419
                     /gene="rpsJ"
                     /locus_tag="P5179_00830"
     CDS             111..419
                     /gene="rpsJ"
                     /locus_tag="P5179_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRS93797.1"
                     /translation="MAGQKIRIRLRGYDHRALDRSVDEIVRTAKRSGARIKGPIPLPT
                     RRSVTTVLRSPHVNVKSREQFMLAVHKRLLDIYDSTPQTVDALMKLELPAGVDVEIQV
                     "
     gene            439..1062
                     /gene="rplC"
                     /locus_tag="P5179_00835"
     CDS             439..1062
                     /gene="rplC"
                     /locus_tag="P5179_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRS93798.1"
                     /translation="MKQLVGRKIGMTRIFDGAGREISVTVVEVQPHVVIGHRTAEKNG
                     YDAVIVGVEEDSKRRAPRQIAGQYPENVSPRKLIREIKLESAPEIGSTVDASVFSVGD
                     RVKVVGTSKGRGFAGFVKRHHFKGGPNTHGSMSHRLPGSIGSSAYPSRVIKGVKGAGH
                     LGNQSSTVKGLRVVEVDAERNLLVLSGCVPGWRNGLITISSDDRGKA"
     gene            1067..1741
                     /gene="rplD"
                     /locus_tag="P5179_00840"
     CDS             1067..1741
                     /gene="rplD"
                     /locus_tag="P5179_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRS93799.1"
                     /translation="MNAVRFDEAGAEAGRVDLPATLFGAEPNLDVVHKAVVTYLANQR
                     QGTAKTKTRSEVAGTNKKPFKQKKTGQARRGDMKTNVQRGGGVYGGPIPRDFRQKLPR
                     KMRQLALRSALSIRAKEDAVSTIADISLESGKTRDVAALLKNVGLQDKRVLFVADKSP
                     ATLQRASRNIENFWLTSVGSLSTYAVMWAERVVFTESALARLNEAGLNEGSLAGAGVS
                     EAGGAQ"
     gene            1738..2040
                     /gene="rplW"
                     /locus_tag="P5179_00845"
     CDS             1738..2040
                     /gene="rplW"
                     /locus_tag="P5179_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRS93800.1"
                     /translation="MSSPYTIVKRPVVSEKVNLQQERFNQYTFEVDRASNKHDIQRAI
                     EELFSVKVTSIRVIHVRGKKRRMGMRYRKLGRTASWKKAIVTLEKGQTIDILGERV"
     gene            2043..2879
                     /gene="rplB"
                     /locus_tag="P5179_00850"
     CDS             2043..2879
                     /gene="rplB"
                     /locus_tag="P5179_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRS93801.1"
                     /translation="MPFKKFRPLTPSLRYTTTDTFEDVTTGTPERSLVEKLHRTSGRN
                     NSGRITSRRRGGGHRRLYRVIDFKRDKFGIPAKVKSIEYDPNRTARIALLVYADGEKR
                     YIIAPDGIAVGDELMSGPTAPVRPGNALPLTHIPAGSTVHNVEMKPGKGAALARSAGA
                     LVQLISREGDMARLRMPSGEVRLVPSVCMATLGQVGHVDRDDRSLGKAGRNRWLGRRP
                     SVRGVAMNPVDHPHGGGEGRTSGGGGKGSHPRTPWGVITKGKKTRNPKKSNRLIISRR
                     TK"
     gene            2891..3196
                     /gene="rpsS"
                     /locus_tag="P5179_00855"
     CDS             2891..3196
                     /gene="rpsS"
                     /locus_tag="P5179_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRS93802.1"
                     /translation="MARSIKKGPYIEPSLQKKVDELNRTGEKRVIKTWSRRSTISPDF
                     VGHTLGVHNGNKFIPIYVTENMVGHKLGEFAPTRIFRGHPGSGGGGEKTAKVAPSAK"
     gene            3225..3587
                     /gene="rplV"
                     /locus_tag="P5179_00860"
     CDS             3225..3587
                     /gene="rplV"
                     /locus_tag="P5179_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRS93803.1"
                     /translation="MALIARAVSKNQWASARKVRLVADMVRGKTVGTALNVLHFSPSS
                     ASLQVEKTVRSAMANLLEKEQGQRLVEDDVVISQITVDGGMIYKRIRPRAMGRAFRTR
                     RRTCHITVEVAARERTER"
     gene            3603..4319
                     /gene="rpsC"
                     /locus_tag="P5179_00865"
     CDS             3603..4319
                     /gene="rpsC"
                     /locus_tag="P5179_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRS93804.1"
                     /translation="MGQKTHPTGLRLGVLRTPERPNGLWKSRWYGGRDFPALLKEDQM
                     LRAYVLQRLQKASVSLIEIERQPKRISITVHTARPGIVIGRKGQEVDKLRDELRHLTK
                     KDVFINIEEIKKPELDAELVAQNIRRQLEGRVLFRRAMKKALENTMRMGAQGIRVVCS
                     GRLGGAEMSRTERYIRGRVPLHTLRADIDYALVQALTTMGMIGVKVWICRGEQIGDDF
                     PTAPRRAEGTASGMSEGARR"
     gene            4326..4748
                     /gene="rplP"
                     /locus_tag="P5179_00870"
     CDS             4326..4748
                     /gene="rplP"
                     /locus_tag="P5179_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRS93805.1"
                     /translation="MLMPKRVKHRKQQRGRRKGLALRGSAIHFGQYGLQAVEPAWVTD
                     RQLEAARVAMTRYIKRGGKVWLRVFPDKPVTAKPAETRMGKGKGVPDHWVAVIKPGRI
                     LFEMEGIDEATAQEAMRLANSKLPVKTRFIQRSETVQG"
     gene            4754..4990
                     /gene="rpmC"
                     /locus_tag="P5179_00875"
     CDS             4754..4990
                     /gene="rpmC"
                     /locus_tag="P5179_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRS93806.1"
                     /translation="MKTRDIRKMSIEEVRVHLREAEEEVFRLRFRAATEQLESPIMLR
                     HRRRDIARMVTIIREHEQGVRTLAQRESVEGATS"
     gene            4987..5250
                     /gene="rpsQ"
                     /locus_tag="P5179_00880"
     CDS             4987..5250
                     /gene="rpsQ"
                     /locus_tag="P5179_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRS93807.1"
                     /translation="MTSETRAHRKTRVGRVVSAKPDKTIVVVIDRLVRHSLYERIVRR
                     TKKLYVHDEQNEAHVGDTVEAMETRPLSKTKHWRLVRIVERAR"
     gene            5266..5634
                     /gene="rplN"
                     /locus_tag="P5179_00885"
     CDS             5266..5634
                     /gene="rplN"
                     /locus_tag="P5179_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRS93808.1"
                     /translation="MIQQQTNLVVADNTGAKRAMCIKVLGGTRARYATVGDQIIVAVK
                     EAIPGGAVKKGDVARGVVVRVSRATRRPDGSHVRFDDNAVVLINDQGEPRGTRIFGPV
                     ARELREHKFMRIVSLAPEVV"
     gene            5659..6003
                     /gene="rplX"
                     /locus_tag="P5179_00890"
     CDS             5659..6003
                     /gene="rplX"
                     /locus_tag="P5179_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRS93809.1"
                     /translation="MDILKGDLVEVISGDSRGPRRGEGKRGKVLFVDRAKGKVIVEGV
                     NIIRRHTKPSRENPQGGIVEKEAPISVSKVLLVSPRANRGVRVRHQRLEDGTRVRICT
                     KTGDMIPPPSKS"
     gene            6203..6751
                     /gene="rplE"
                     /locus_tag="P5179_00895"
     CDS             6203..6751
                     /gene="rplE"
                     /locus_tag="P5179_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRS93810.1"
                     /translation="MPPRLRERYKAEIVPQLTKRFSYKNPMQVPRVLKVVVNMGVGEA
                     IQNPKAVDSAVADLQQIAGQKPSVRRARKSVSNFKLRAGVPIGCAVTLRGVRMWEFLD
                     RLLTFAVPRIRDFRGIPRRGFDGRGNFTFGVKEQLIFPEIDYDTVDQIRGMDISIVTT
                     ARTDEEAFELLSGLGMPFARQA"
     gene            6780..6965
                     /locus_tag="P5179_00900"
     CDS             6780..6965
                     /locus_tag="P5179_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRS93811.1"
                     /translation="MAKKSWMIKSARKPKFKVRAYNRCHRCGRSRGFLRKYGVCRICF
                     RELALRGDIPGVVKASW"
     gene            7001..7399
                     /gene="rpsH"
                     /locus_tag="P5179_00905"
     CDS             7001..7399
                     /gene="rpsH"
                     /locus_tag="P5179_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRS93812.1"
                     /translation="MSMTDPIADFLTRIRNACKAGHRKVDIPASTLKREVARILLEQG
                     YVEDVYVVDDGKQGMLRIYLRYLRDGKPVIRGLARRSKPGLRRYNGAQTMDRVLNGLG
                     IAIVSTSKGVMTDAECRRIGVGGEVLCHVW"
     gene            7410..7979
                     /locus_tag="P5179_00910"
     CDS             7410..7979
                     /locus_tag="P5179_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRS93813.1"
                     /translation="MSRIGRRAIPLAKNVAVAISGDTLTAKGPLGEVSVKIPAGVNVK
                     IDSDQVTVEPEIGASPGAWGLARTIIANAVEGVSKEFERRLFVVGVGYRAEADGSKLT
                     LSVGHSHPVTIQADRGVTFAVEPAITLPGKKDNYPAIPVVVKGVNKEAVGDYAAKVRA
                     VKPPEPYLWKGIRYQNERMRTDKAGKTAK"
     gene            8021..8368
                     /gene="rplR"
                     /locus_tag="P5179_00915"
     CDS             8021..8368
                     /gene="rplR"
                     /locus_tag="P5179_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRS93814.1"
                     /translation="MSERRIRRVKRWRLAKPIRGTAERPRLVVSRGLRTIQGHFVNDE
                     IGKVICGASTASKKLAIAGRGCEAAKSLGKELARVAREHGITRTVFDRNGYLYHGRVK
                     ALAEGAREGGLEF"
     gene            8390..8989
                     /gene="rpsE"
                     /locus_tag="P5179_00920"
     CDS             8390..8989
                     /gene="rpsE"
                     /locus_tag="P5179_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRS93815.1"
                     /translation="MARDGGYSRGGNVEESEFVETVVKVKPVSKVTKGGRDRRFNALV
                     VVGDKKGRVGVGMGKAREVSEAIRKGTQIAQRSIQRVSVGVTIPHEVWGRCGAGLVFL
                     RPATPGTGLIAGSAARAVLEAAGVENVLSKSVGSANALNVARATLDALAKLRTFTQVR
                     SERRLIPPETVAAETVDIAPAENAENEAPVETPNEGAEA"
     gene            8986..9165
                     /gene="rpmD"
                     /locus_tag="P5179_00925"
     CDS             8986..9165
                     /gene="rpmD"
                     /locus_tag="P5179_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018663943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRS93816.1"
                     /translation="MKKITVTLYRSPIGRVAYQKRVVKGLGLHKVGQTVEHEDKPDIR
                     GMVAKVPHLVRINEA"
     gene            9179..9631
                     /gene="rplO"
                     /locus_tag="P5179_00930"
     CDS             9179..9631
                     /gene="rplO"
                     /locus_tag="P5179_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRS93817.1"
                     /translation="MKLGDLVPAEGARKNRKRVGRGNGSGHGTFSGRGCKGTKARSGT
                     GRMRVGFEGGQMPIQRALPARGFRNPAKKSFQLVNVGDIAARTLGDRVDAAALHAAGL
                     IRSAVEPVKVLGDGELNRAVTVVATMFSRTAREKIAAAGGTAEESTGA"
     gene            9624..10964
                     /gene="secY"
                     /locus_tag="P5179_00935"
     CDS             9624..10964
                     /gene="secY"
                     /locus_tag="P5179_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRS93818.1"
                     /translation="MLERFRNIFNIPELRNRILFTFGVLIVYRIGGAIPTPGVNSAAL
                     AQAFSARARDLFAVYDLFAGGAFKQATIFALGIMPYISASIIFQLLGSVWAPIQQLQK
                     EGQEGQKKINQYTRYLTVALAAVQSLGVSQFLIQNGAALDPGIGFRLMVMLTMTTGTV
                     FVMWLGEQISERGIGNGISLIVFIGIVARFPHAVIGQLRVMLDPDSGGVIATMALFAL
                     MFVITAAVVLITQGTRRIPVRYASQRVVGGRVYGGQSTHLPLKVNAAGVIPIIFAQSI
                     VLVPTTVLQFAGTATPESETLQMVFGWFQPTSLIYWTFYSLLIIFFSYFYTAIIFNPV
                     DVADNLKRNNGFIPGVRPGKKTAEYIDRVLNRITLPGSIYLALIAALPFLIISIFQSF
                     RVSVVPDFHDFFGGTGLLIVVGVALDTLDKVESHLLVRHYDGFMKGSRLRSRRR"
     gene            10994..11644
                     /locus_tag="P5179_00940"
     CDS             10994..11644
                     /locus_tag="P5179_00940"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRS93819.1"
                     /translation="MRLILLGGPGSGKGTQGAVLAQKFAIPTISTGDLFRKNIADNTP
                     LGQKVKQYTDQGALVPDAVVLDMVFDRLVQPDCAAGYLLDGFPRTIPQAEALTKWLSE
                     KNAAIDHVVLIDVDDEVIVDRLSSRRTCESCGAIVSVKFSPPKKEGVCDLCGGKLVQR
                     ADDNPGTVRDRLQVYHKNTEPLIAYYRQQGLLRAVDGSASPELVTKAILAAVGAKS"
     gene            11660..11878
                     /gene="infA"
                     /locus_tag="P5179_00945"
     CDS             11660..11878
                     /gene="infA"
                     /locus_tag="P5179_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRS93820.1"
                     /translation="MPKEDSIEVEGTVTKALPNASFKVRLENGHEVSAHISGKMRVNF
                     ITIKPGDKVKLELSPYDLTRGRITYRHR"
     gene            11903..12019
                     /gene="rpmJ"
                     /locus_tag="P5179_00950"
     CDS             11903..12019
                     /gene="rpmJ"
                     /locus_tag="P5179_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRS93821.1"
                     /translation="MKVRSSVKKLCANCKIIRRRGVVRVICKSSPTHKQRQG"
     gene            12068..12454
                     /gene="rpsM"
                     /locus_tag="P5179_00955"
     CDS             12068..12454
                     /gene="rpsM"
                     /locus_tag="P5179_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRS93822.1"
                     /translation="MARIAGVDLPREKRVDIALTYIYGIGPASSAKILQETGVDPSRR
                     MNQLTEDEIARLRSVIENQFRVEGALRSEIEMNIRRLQEIGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRAVAGARKAGKKTK"
     gene            12513..12911
                     /gene="rpsK"
                     /locus_tag="P5179_00960"
     CDS             12513..12911
                     /gene="rpsK"
                     /locus_tag="P5179_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRS93823.1"
                     /translation="MASPTGRARKGRKKDRKVEATGVAHVKATFNNTQVTLTDKDGNL
                     ISWSSAGKAGFKGSRKSTPFAAQSAASQAAREAMDLGLRKVEVLVKGPGVGREAAVRA
                     LQAAGLEISAIRDVTPIPHNGCRPPKRRRV"
     gene            12937..13566
                     /gene="rpsD"
                     /locus_tag="P5179_00965"
     CDS             12937..13566
                     /gene="rpsD"
                     /locus_tag="P5179_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRS93824.1"
                     /translation="MARYDGPVCRLCRREGTKLFLKGERCNSEKCSLDRRNYAPGQHG
                     QGTQRKVMEYGRQLREKQKARRTYGVLERQFRRYYEMAVRIPGVTGENLLRLLEQRLD
                     NVVYRLGFAPSRAAARQLVRHRHIEVNGRPVSIPSFLVRPGMTVSVRQGSKEMPSIHE
                     SLRQLGKSREMSWLVVDKVALSGQMVQLPSREDIQIPLEERLIVELYSK"
     gene            13732..14724
                     /locus_tag="P5179_00970"
     CDS             13732..14724
                     /locus_tag="P5179_00970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRS93825.1"
                     /translation="MPDAVEVDQASTQTYAKFVLQPLERGFGTTLGSSLRRTLLSSME
                     GAAILAVRIEGVQHEFTVIPGVVEDVPELVLNLKQVRLRLHTDEKKTIHVRKKGPGEL
                     VAGDLAVDSAVEIVNPKLVLATLSEGAEIAMEVDLGKGRGFLLAEEMESEDIPIGTIP
                     IDAVFSPITKVDFEVGNARIGQKTDYDKLTLEVWTDGTTTPRSAVAFAARLLREHLQL
                     FMGESEREETGARGVLAAAPTSVLDTRVEDMDLSMRSINCLRAAGITTVQELVLKSEN
                     DMLKYRNFGRKSLIELTEKLESLGLRFGMSPEELAEVRAQLSGGSAPEEKSEEA"
     gene            14727..15212
                     /gene="rplQ"
                     /locus_tag="P5179_00975"
     CDS             14727..15212
                     /gene="rplQ"
                     /locus_tag="P5179_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRS93826.1"
                     /translation="MRHGNKGRRLGVTTPHRRSMMANLAVSLIQHGGINTTEARAKEL
                     RPFVEELVTLAKRGDLHARRLVTARLRDPGATKKLFDEWAPKFVSHPTKAGDGWNGGY
                     VRILRLGLRKGDSASISLVQFVTAESAESSASRKPAQEKKSEPAKSSSEKDNAASEET
                     S"
     gene            15301..16713
                     /gene="rlmD"
                     /locus_tag="P5179_00980"
     CDS             15301..16713
                     /gene="rlmD"
                     /locus_tag="P5179_00980"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRS93827.1"
                     /translation="MSDTTTHDSGMAASATNGAVRPGMIVPAIIEGIAPDGRGMASAS
                     GRQLLVRGALPGDEVDARVFRRRGGRLEATCVSVRREGYPRREPRCAHTEQCGGCTWQ
                     QWEPGIQHEMKRRIVREALMRAMPDAAIEVLPVVAAGPEFGYRNKMEFTFGKDETGAV
                     TLGLHRVGRFDATFDVKRCWIAPEGMNAVRAWVCEWARRNALVPYDPRRFAGALRHLV
                     LREASATGEVMANLVCATEEVAGLDELSEGLPRELPQVKSLLFSVNTRRGDTAHAETT
                     VLLSGRPAISERIGDMEVTVSPISFLQTNSAGAERLYSVVSEVAALSGEERVLDMYCG
                     IGLIGISLAPRAREVVGLEQVPSAVQDANAMRERLGYTHVQFRQGDAEAVLPAWADRG
                     ERFDVVIVDPPRAGLHPKALAALVRMSPRLIVYVSCNPVTLAANLADLSSSGYRIGAA
                     HPVDLFPHTPHVETVVRLEK"
     gene            16737..18200
                     /locus_tag="P5179_00985"
     CDS             16737..18200
                     /locus_tag="P5179_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS93828.1"
                     /translation="MEYVVEPEKRVPVVYDVDVCVAGAGVSGVFAAIAAARNGARTVV
                     VDRFGSVGGNVGPGMIVNGQMLPGSPHPDVPFTKTVYPGLFGLAEEFINRYAALGGGL
                     LQPFTKGANFSRGASVASYLLQEMLEESGASILLPTFVADPIKEGDRVCGVFVENKSG
                     RAAVRAKVVIDATGEADVARRAGAPILLPKNEYTAIDGHAPTGMGTYYVVGGIAWDRF
                     RSAQKGNEATEDDIAWGTKTIGAALTKAHSYVLPYLRKAVEAGEYEPVAKVMINGKSI
                     SVQGGGVGQFEGNELGHGQVQVERTEEVDAGDGLHISALGAAVQAYAFEKVQFFSRYV
                     PGFENAYLITVAPFLGSRGGPCIEGEYTLTMEDCVAGRRFDDVIYLYGEFRALRKTAE
                     AGAPKWVDVPYRVLVPQGVDGLLATGRSASGIPDTLLRNRLAVKVMGEACGIAASMCA
                     AKGVEPRKLEVRELQNALLDAGFYLGDRARLKELSLT"
     gene            18244..20034
                     /locus_tag="P5179_00990"
     CDS             18244..20034
                     /locus_tag="P5179_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS93829.1"
                     /translation="MRTYVGVVVSGLALLASSCAKQGPAVVPVQRVEMVAAADLPVLG
                     TRALPTDPQKFSFVILGDKSGGLDRNWPLFDRAVQEINLLKPDFIITVGDQIEGSNDS
                     ASVVAQWELFRSHAGKFEAPLFMLPGNHDAADLMGYEYWKRNVGRTYYDFTYKGCYFL
                     VLNTQEASREFPQFGEAQIAWAVDALQRHPNPRHTFVFMHYPAWFASDPERQRGGISA
                     EWQRIETALGNRKYSVIAGHTHNLMWATRDGNRYLVHGATGATLTPSPVKQVGAFHHY
                     AKVTVEADQAHIAIVEPGNIWPETIAPLEFQRNIGRFVRVQSSRRDLADGRIAMDVTA
                     ELNNHVGDTVSVALIPVLGAAGVWAPSADSLVSVLPDGGTDSLTLTFVGSRDNWYPTP
                     AWKMVVRYQGKEVDTYGPSPLNPFSESRATVLPEWRALGTFPVGPINRDVMPGNPRAG
                     VPGMFVPRAPDAGWNGGAPITVDGRTYSWVTARPDSTGRVSLDRVFGIVDLSLAYLST
                     AIYSPVDQRVPIQIGVDNFFQLFLNGQMVPGGEAYGMPYERKILPLDLKAGWNSLYLK
                     VVNNRGNWGVEASVIDLRGNLRFAPYPGQN"
     gene            20097..21911
                     /gene="aspS"
                     /locus_tag="P5179_00995"
     CDS             20097..21911
                     /gene="aspS"
                     /locus_tag="P5179_00995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRS93830.1"
                     /translation="MGGSGTDRLSGWRRTHRCGDLRASDIGLDVILMGWASSHRDHGG
                     VIFVDLRDRWGITQVVFDPAVSKEVHKRAEHIRQEYVIAVKGRVRARMEGMANAKLAT
                     GEIEVICNELRVLNSAETPPIQVDGPPANEEIRLKYRYLDLRRPIMQRNLELRHAAAQ
                     ATRRFLCAQDFVEIETPTFVSSTPEGARDYLVPSRVWPGRFYALPQSPQIYKQLLMVA
                     GMDRYFQIVRCYRDEDLRADRQPEFTQIDVEMSFVEEADVMRVAEGITRSVYKETIGY
                     DVPDSFPHLDYADAMSRYGSDKPDIRFGLEMVDVTDIAAESDFKVFRAVTEAGGIVSA
                     INVKGGASFSRKQVDDMTPFVAQYGAKGAAWMKVAERGLESNIAKFFNEGVQNALRER
                     LSAEQGDLLVFVADQAPIVYQALGNLRLKFGNDLNLIDKSKFAFLWVTNFPLLSWDEE
                     ENRWVANHHPFTSPVLEDLDLFVSGGDPGRIRARAYDLVINGTEVGGGSIRIHQRHVQ
                     QQMFDLLGISPEEAERRFGYLLNAFRYGAPPHGGIAFGFDRMVMQLAGQESIREVIAF
                     PKTNTAASPMDGSPSEVDERQLRELGLRLRHVKHQGTA"
     gene            22265..>22612
                     /locus_tag="P5179_01000"
     CDS             22265..>22612
                     /locus_tag="P5179_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRS93831.1"
                     /translation="MSNRRREFRGQIFALMMFLGVSGVASAQTTTMPNVVGMDIASAT
                     AAVKGTIARFATLYVEPTDDPAAVGKIDGQYPTAGSELSSSTVVTLTARRANTPGLLT
                     LASPAKGATDVSVG"
BASE COUNT         4731 a         5896 c         7648 g         4337 t
ORIGIN      
        1 cgtgaaggcg gcagaaccgt cggcgcaggc tccgttacaa aaattctgca gtaaggattt
       61 cgccgcggga atggctccgc gggtgttgaa aaccataaga gggcacgcac gtggctggtc
      121 agaaaattcg cattcgcctg cgcgggtacg accacagggc gcttgatcgg tcggttgacg
      181 agatcgtgcg cactgcgaag cgttccggtg cgcggatcaa agggcccata cccctcccga
      241 cgcgccgttc agttacgacc gttctgcgtt cgccgcacgt gaacgtcaaa tcacgtgagc
      301 agttcatgct tgcggtgcac aagcggcttc tggatatcta cgattcgact ccgcagacgg
      361 ttgacgctct gatgaagctt gagcttcccg ctggcgtgga tgttgagata caggtttgat
      421 acgcacggtg gaccagacat gaagcaattg gtaggacgca agatcggaat gacgcgcatc
      481 tttgacggcg cggggcggga gatctctgtc acggtcgttg aggtgcagcc gcatgttgtg
      541 atcggccacc gcacggcgga gaagaacggc tatgatgccg ttattgtcgg cgtggaagaa
      601 gattcaaagc gacgtgcgcc ccgtcagatt gcagggcaat acccggaaaa cgtcagcccg
      661 aggaagctca ttcgggagat taagctcgag tcagcgcccg agatcggctc cacggtagac
      721 gccagcgtgt tttccgtggg tgacagggtg aaagtggttg gcacaagcaa agggcgcggt
      781 tttgccggtt tcgtgaaacg ccaccacttc aagggcggac ccaatacgca cgggagcatg
      841 agccaccgct tgccggggtc gatcggctcg tcggcgtatc ccagccgcgt catcaagggc
      901 gtgaagggtg ccggacacct gggcaaccag agcagcactg tcaagggttt gcgcgtggtg
      961 gaagttgatg cagaacgtaa ccttctggtg ctgagcgggt gcgtccccgg atggcgcaac
     1021 ggtctgataa ccatttcttc ggacgatcgt ggtaaggctt gaaactatga acgcagtgcg
     1081 gtttgatgaa gcgggagccg aagcggggcg ggtagacctg cccgcgacgc tcttcggggc
     1141 cgagcccaat ctggacgtgg tgcataaggc agtggtgacg tacctcgcga atcagcgtca
     1201 gggtaccgcc aaaaccaaga cccgttcgga agttgccggc acgaacaaga agcccttcaa
     1261 acagaaaaag accgggcagg cgcgccgcgg cgacatgaaa acaaacgtgc aacgcggggg
     1321 cggtgtctac ggcggaccaa ttccgcgaga ttttcgccag aagctgccca ggaagatgcg
     1381 gcaactggct ctgcgttccg ccctttcgat ccgggcgaag gaagacgcgg tttcgacgat
     1441 agcggatatc tcgctggaat cgggaaagac gcgggacgtg gcggcgttgc tcaagaatgt
     1501 cggattgcag gacaagcgcg tgttgttcgt cgccgacaag tccccggcga cactgcagcg
     1561 ggcaagccgg aatatagaga acttctggtt gacgtctgtc ggttcattga gcacgtacgc
     1621 ggtgatgtgg gcggagcggg ttgtgtttac cgagtcagcc cttgcccgtc tgaatgaggc
     1681 aggcctcaac gagggaagcc tcgccggggc aggcgtcagt gaggcaggag gtgctcaatg
     1741 agttccccct atacgatagt caagcgcccc gtggtgtcgg agaaggtgaa tctccagcag
     1801 gagcgtttca atcaatacac cttcgaagtg gatcgcgctt ccaacaaaca cgatatccag
     1861 cgcgccatcg aagagctttt cagtgtgaag gttacgtcga tccgggtgat ccacgtgcgc
     1921 ggcaaaaagc ggcgcatggg catgcggtac cggaaactcg gaagaacggc gtcctggaaa
     1981 aaagcgatcg tcacgctcga aaaagggcag acgattgata ttctcggcga aagggtatag
     2041 cgatgccgtt caagaagttt cgcccgctca ccccaagctt gcgatatacg acgacggata
     2101 cgttcgaaga cgtaacgacg ggcacgccgg agcgttcgct ggtggagaaa ctccaccgca
     2161 cgtcgggccg caataactcc ggaagaataa cctcccgtcg acgtgggggc gggcatcgcc
     2221 gtctgtatcg cgtgattgat ttcaaacggg acaagttcgg catccccgcg aaggtgaagt
     2281 cgatagaata tgatccgaac cggaccgcgc ggattgccct gctggtgtac gcggacggcg
     2341 agaagcggta catcattgct ccggacggca tcgcggtcgg cgacgaattg atgtccgggc
     2401 cgacggcgcc cgtgcggccg ggaaatgcgc ttcctttgac gcacattcct gcgggttcca
     2461 ccgtgcacaa tgtggagatg aagccgggca agggagcagc tcttgcgcgc agcgcgggtg
     2521 ctctcgtgca gctcatttca cgtgaaggtg acatggcgcg gcttcgcatg ccttccgggg
     2581 aagtgcggct ggttccctca gtgtgcatgg caacgctggg ccaggtgggc cacgtggatc
     2641 gcgatgaccg ctcacttggg aaagccggcc ggaatcgctg gctgggccga cgcccgagcg
     2701 tccgcggtgt tgcgatgaac ccggtggatc atccccatgg cggtggtgaa gggcgcactt
     2761 cgggcggtgg tggcaaaggc tcccatccga gaactccctg gggcgtaatc accaagggca
     2821 agaagacgcg gaatcctaag aaatccaatc gcctcatcat cagtcggcgc acgaagtaga
     2881 ggaatggtac atggcacgtt ccattaagaa ggggccctac atcgagccca gtctgcagaa
     2941 gaaggttgac gagctcaacc gcacgggtga gaaacgggtt atcaaaacgt ggtcgcggcg
     3001 ttcgacgatt tcgccggatt tcgtggggca cacgctcggg gtgcacaacg gcaacaagtt
     3061 catccccatt tacgtgacgg aaaacatggt tgggcacaag ctgggtgagt tcgcgccgac
     3121 acggattttc cgggggcacc cgggttccgg tggcggtggc gaaaagacag cgaaagtagc
     3181 gccctcggcc aagtgatacc agtgtataga aggaatcggg aacgatggca ctcatagctc
     3241 gcgctgtcag caagaaccag tgggcatctg cccgtaaggt gcgccttgtc gcggatatgg
     3301 tgaggggaaa gacagtcgga accgcattga acgtgctcca cttttccccg tcttccgcca
     3361 gcctgcaggt cgaaaagacg gtgcgctcgg cgatggcgaa cctgctggaa aaagaacagg
     3421 gccagcgtct tgtggaagat gacgtggtta tttcgcagat aacagtagac ggcgggatga
     3481 tttacaagcg gattcgtccg cgggcgatgg gtcgtgcgtt ccgcacgcgc cgccggacct
     3541 gccacattac ggttgaagtc gctgcacgcg aacgcacaga acgctaggaa gggagcgtct
     3601 tagtgggaca gaaaactcat ccaacgggac ttcgactcgg agtgctccgg actcccgaac
     3661 gtccgaatgg actttggaaa tcgcgctggt acggtgggcg cgacttcccg gctcttctga
     3721 aggaagacca gatgctccgc gcatacgttc tgcagcgcct tcagaaagca tcggtatcgc
     3781 ttatcgagat tgagcggcag ccgaagcgga tcagcatcac cgtccacacc gcccgcccag
     3841 ggatcgtgat cgggcgtaag gggcaggaag tggacaaact gcgtgacgag ctccgtcacc
     3901 ttacgaagaa agacgtgttc atcaacatag aagagatcaa gaaacccgaa ctggatgcgg
     3961 aactggttgc tcagaacatt cgtcggcagc ttgaaggccg cgtgcttttc cggcgcgcca
     4021 tgaagaaggc gctggaaaac acgatgcgca tgggagccca gggaattcgc gtggtctgtt
     4081 ccgggcggct tggtggtgcg gaaatgtctc gcaccgaacg gtacatccgt gggcgcgtgc
     4141 cgctccacac gctgcgggcg gatattgatt acgcgctggt tcaggcgttg acgaccatgg
     4201 gtatgatcgg cgtgaaagtg tggatttgtc gtggcgagca gatcggcgat gacttcccga
     4261 ctgcaccccg gcgtgcggaa ggcacggcaa gcggcatgtc cgaaggcgcg aggagataaa
     4321 cagcgatgtt aatgccgaaa cgtgtcaaac accggaaaca gcagcggggg cggcggaaag
     4381 gccttgctct tcggggctcc gcgattcatt tcgggcaata cggccttcag gcggtcgaac
     4441 cggcgtgggt gacggaccgc caactggaag ccgctcgtgt tgccatgacg cgctacatca
     4501 agcgcggagg gaaggtctgg ttgagggtgt ttccggacaa gccggttacc gcgaaacccg
     4561 cggaaacccg tatgggcaaa gggaagggtg tgcctgacca ctgggttgca gtgatcaaac
     4621 ccggtcggat cctgttcgag atggaaggta ttgacgaggc taccgcacag gaagcgatgc
     4681 ggctcgccaa ttcgaaactt cccgtcaaga cgcggttcat tcagcgttct gaaacggttc
     4741 aggggtgacg gtgatgaaaa cacgtgacat tcgcaagatg tccatcgaag aggttcgcgt
     4801 gcacctgcgt gaggcagagg aagaggtgtt ccgcctccgt ttccgcgccg ccaccgagca
     4861 gttggagagc cctatcatgc tccgacaccg gcgccgggat atcgcccgga tggtcacgat
     4921 catccgcgaa cacgagcagg gcgtccgtac gcttgcccaa cgggaatcgg ttgaaggggc
     4981 gacatcatga caagtgaaac tcgtgcccat cgcaaaacac gcgttggccg tgtggtaagt
     5041 gcaaagcctg acaagacgat agttgtggtg atagaccgcc tcgtgcgtca ctcgctgtat
     5101 gagcgcatcg tccgccggac gaagaagctt tacgtacacg acgaacaaaa tgaagcgcac
     5161 gtgggtgaca ccgtggaagc tatggagacc aggccgctga gcaaaaccaa acactggcgg
     5221 ctggtccgta ttgtggaacg tgcccggtag gaaggctttt tcgccatgat tcagcaacag
     5281 acaaatctcg tggtggccga caataccggc gcgaagcgtg ccatgtgcat caaggtgctc
     5341 ggtggaacac gtgcgcggta cgccacagtc ggggatcaga tcatagtcgc agtgaaagag
     5401 gcgatccccg gcggggcagt caagaagggc gacgtcgcgc gtggtgtggt ggtgagagtg
     5461 tcccgcgcaa cgcggcggcc ggatggctcc catgttcgct ttgatgacaa cgcggtggtg
     5521 ttgatcaatg atcagggcga accgaggggt acgcgtattt ttggtccggt tgctcgcgaa
     5581 cttcgtgagc acaagttcat gcgcattgtt tcgctggctc cggaagttgt ctgagcacga
     5641 gccgagggga ttgaagtcgt ggacatattg aagggcgatc tcgtcgaagt gatcagcggg
     5701 gacagccgtg ggcccaggcg aggcgagggc aagcggggca aagtgctttt cgttgatcgg
     5761 gccaaaggaa aagtgattgt cgagggtgtg aacatcattc gtcggcacac aaaaccctcg
     5821 cgtgagaatc cgcagggtgg catcgtggaa aaagaggccc cgatttcggt gtcgaaggta
     5881 ttgcttgtca gcccgcgagc gaatcgcggc gtgcgagttc gccatcagcg cctggaagac
     5941 ggcacaaggg tgcgtatttg cacaaagacc ggcgacatga tacctccgcc gtccaagtca
     6001 tgaaggtggg gagcggaaac tgatggcaaa agaggaaaca aagaaaaaag cggccaaagc
     6061 cgaagcggga gccgacgcaa agggcgcgac gaagaagaag gcgcccaaag agcaggctgc
     6121 cgcggaagcg gctcccaaag ggaaaaaagc cgcgggtgcg aagtcgcagg aaccgaaggc
     6181 cgccgaaccc aaggctcagg cggtgccgcc gcgattgcgg gaacggtaca aggcggagat
     6241 cgtgccgcag cttaccaagc ggttctccta caagaacccg atgcaggttc cgcgggtgct
     6301 gaaagtagtc gtcaacatgg gtgtgggcga ggctattcag aacccgaaag ccgtggacag
     6361 cgccgttgcg gacctccagc agatcgccgg tcagaaaccg agcgtgcggc gtgcgcggaa
     6421 gtcggtgtcg aatttcaaac tgcgcgcggg tgtcccgatc gggtgtgcag ttacgcttcg
     6481 gggcgtgcgc atgtgggaat tcctggaccg gcttttgacg tttgccgttc cccgtatcag
     6541 ggatttcagg ggtattccgc ggcgtgggtt cgatggccgg ggcaatttca cgttcggcgt
     6601 gaaagagcag ctgatcttcc cggaaataga ttacgacacg gttgaccaga ttcggggcat
     6661 ggatatttcg attgtcacta ccgcgcgcac tgacgaagaa gcgttcgaac tgctgagtgg
     6721 tttgggtatg ccgttcgcgc gccaggcctg acgggcgaac gcggaaagcg agggttctcg
     6781 tggcgaaaaa gagctggatg atcaaatcgg cgcgtaaacc gaaattcaag gtccgggcat
     6841 ataaccgttg ccaccgttgc ggaagatcgc gcggatttct gcggaaatac ggcgtgtgcc
     6901 ggatctgctt ccgcgagctc gcgctccgcg gggatatccc cggcgtggtc aaggccagtt
     6961 ggtagccgta cgtgcggttc agaattagag gaacagaact atgtcaatga ccgatccgat
     7021 cgcggatttc ttgacgcgaa tcaggaatgc gtgcaaagct gggcaccgga aggtagatat
     7081 cccggcgtcg acactgaagc gcgaggtagc gcgcattctg ctcgagcagg gctacgtcga
     7141 ggatgtctac gtagttgatg acgggaaaca gggcatgctg cgcatttacc tgcggtacct
     7201 gagagatggc aaaccggtca tccgcgggtt ggctcggcgt tcgaagcctg gtttgcgccg
     7261 ttacaacggt gcgcagacca tggatcgtgt tctgaacggt ctgggaatcg cgattgtgag
     7321 cacgtcgaaa ggtgtgatga cagacgctga atgccgtcgc atcggagttg gtggcgaagt
     7381 tctttgccac gtatggtagg agaaggattg tgtctcgcat aggacgaaga gctattccgc
     7441 tcgcgaagaa tgtcgcggtt gcgatttccg gggatacatt gaccgccaaa ggccctctgg
     7501 gcgaggtgtc tgtgaagata cctgcgggcg tgaatgtgaa aatcgacagc gaccaggtga
     7561 cggttgaacc ggaaatcggt gcaagcccgg gtgcatgggg ccttgcccga acgatcattg
     7621 cgaacgcggt ggaaggtgtc tcgaaggaat ttgaacggcg tttgttcgtg gtcggggtcg
     7681 gttaccgcgc tgaggctgat ggttcgaagc tgacgctgag cgtggggcat tcacatcccg
     7741 tcacgattca ggcggaccgc ggggtgacgt ttgccgtgga acccgcgatt acattgccgg
     7801 gcaaaaagga taactacccg gcgattccgg ttgttgtgaa gggcgtcaac aaagaagcag
     7861 tgggcgatta cgcggcgaaa gtccgggcgg tgaaaccgcc ggagccgtat ctctggaagg
     7921 ggattcggta ccagaacgag cgtatgagga ctgacaaggc cgggaagacg gcgaagtaat
     7981 atactggggc ctgttcgggg ccgttgggga atttaccatc atgagcgaga gacgaatccg
     8041 tcgggtaaag cgctggcgtc ttgcgaagcc gattcgcggc accgccgaaa ggccgcgcct
     8101 tgtcgtcagt cgtggactgc ggacgatcca gggccatttc gtcaatgacg aaatcgggaa
     8161 ggtgatctgc ggggcgtcca ccgcctcaaa gaagctcgcg atcgccggtc gcggttgcga
     8221 ggctgcaaaa agcctcggga aagaactggc gcgcgtggca cgtgaacatg ggattacgcg
     8281 cacggtgttt gaccgcaacg ggtacctgta tcacggacgt gtcaaagcgt tggcagaagg
     8341 cgcgcgagaa ggcggactcg agttctagcg aattcaaaaa ggaacaggca tggcgagaga
     8401 cggtgggtac agcagaggcg gcaacgtaga ggaatcggag ttcgtcgaaa ccgttgtcaa
     8461 ggtaaagccc gtttcgaagg ttaccaaagg cggacgcgac cggcgtttca acgcactggt
     8521 ggttgttggc gacaaaaagg gccgggttgg cgttggcatg ggcaaagcgc gcgaggtctc
     8581 cgaagcgatc cgcaagggaa ctcagattgc gcaacgatcc attcaacggg tttcggttgg
     8641 cgtgacgatt ccgcacgagg tgtggggacg gtgcggcgcg ggtctggtgt tccttcgtcc
     8701 ggcgacgccg ggaaccgggc ttatcgctgg cagcgctgcc cgtgccgtgc tggaagcggc
     8761 gggagttgag aatgttctga gcaagtcggt tggctcggca aatgctctga atgttgcccg
     8821 tgcgacgctg gacgcacttg cgaagctgcg gacattcacg caggtgcgga gtgaacgtcg
     8881 gcttataccg ccggagaccg ttgctgccga gacagtggat atcgcgcctg cggagaacgc
     8941 tgagaatgag gcgccagtag agacgccgaa cgaaggagcg gaagcgtgaa gaagatcacg
     9001 gtgactcttt accggagccc gattggacgc gtggcgtacc agaagcgcgt ggtgaagggg
     9061 ctgggtctgc acaaagtcgg gcagaccgtg gagcacgagg acaaaccgga tatccgaggg
     9121 atggtggcga aggtgccgca tctcgttcgc ataaatgagg cgtagcatgg ccgaacaggt
     9181 gaaattgggc gaccttgtcc ccgcagaagg cgcgcggaag aaccgtaagc gtgttggccg
     9241 gggaaacggg agcgggcacg gaactttcag cgggcgcggc tgcaagggca cgaaagcgcg
     9301 ctcgggcacg ggccgtatgc gtgtcgggtt tgagggtggt cagatgccga ttcagagggc
     9361 attgccggcc cggggtttcc gcaaccctgc aaaaaaatcg tttcagcttg tgaacgtggg
     9421 cgatattgcc gcgcggacac tcggggaccg cgtggatgcc gcggcgcttc acgcagcggg
     9481 tttgatccgc tcggcagtgg agccggtgaa agttctgggc gatggcgaat tgaacagggc
     9541 ggttaccgtg gtggccacga tgttcagccg cacggcgcga gagaagattg ccgccgcggg
     9601 cggcactgct gaggaatcga caggtgcttg aacgcttccg aaacatcttc aacatccccg
     9661 aactgcgtaa caggatactg ttcacgttcg gcgtgttgat tgtgtaccga atcggtggtg
     9721 ctattccgac gcccggcgtg aattcggccg cacttgcgca ggcgttcagc gctcgcgcac
     9781 gggatctttt cgcggtgtac gacctgttcg ccggcggtgc gttcaagcag gcgacgattt
     9841 tcgctctggg tatcatgccc tacatctccg cctcaatcat cttccagttg ctgggttcgg
     9901 tgtgggcacc gattcagcag ctccagaagg aggggcagga ggggcagaag aagatcaacc
     9961 agtacacccg ttatctgacg gtggcgcttg ctgcggtgca gagcctcggg gtgagccagt
    10021 tcctgattca gaacggggcc gcgcttgatc cgggcatcgg gttccggctg atggtcatgc
    10081 tgacgatgac cacggggacg gtgttcgtga tgtggctggg cgagcagatc agcgagcgtg
    10141 gcatcggaaa cggtatcagt ttgatcgttt tcatcggtat cgtggcgcgc ttcccccacg
    10201 cggtgatcgg tcagttgcgt gtgatgctgg acccggacag cggcggggtg atcgccacca
    10261 tggctctttt cgcactgatg ttcgtgatca ccgcggcagt agtgctgatc acccagggga
    10321 cgcggcgaat tcccgtgcgg tatgcgagcc agagggttgt tggcggcagg gtgtacggcg
    10381 gtcagagcac tcatctgccg ttgaaggtga acgcggcggg tgtcattccg atcattttcg
    10441 cgcagtcaat cgtgcttgtc cccacgacgg ttctccagtt tgccggaact gcaacaccgg
    10501 aaagcgaaac gctccagatg gttttcggct ggtttcagcc tacgtcgctg atttactgga
    10561 cgttttactc gctgctgatt atcttctttt cttacttcta cacggcgatc attttcaatc
    10621 cggtggatgt tgcagacaat ttgaagcgga acaacggctt cattccgggg gtccgtcccg
    10681 gcaaaaagac ggccgaatac atcgaccgcg ttctgaacag gatcacgttg cctggttcga
    10741 tctatctggc cctgattgcg gcgcttccgt ttctgatcat ctccatattc cagagtttcc
    10801 gagtgtcagt ggtgccggat ttccacgact tcttcggtgg aacagggctg ttgatcgtgg
    10861 tgggcgtggc gctcgatacg ctggacaagg tggaatcgca tcttctcgtc cgtcattatg
    10921 acggcttcat gaagggcagc aggctccgca gccggcggcg ctgatgttcc cgaagacagg
    10981 aagggggaag ctcatgcgat tgatcctact cggtggcccg ggttccggca aggggactca
    11041 gggtgcggta ctggcgcaga agttcgcgat cccgaccatt tcgaccggtg atcttttccg
    11101 gaagaacatc gcggacaaca cgccgctcgg gcagaaggtg aagcagtata cggatcaggg
    11161 cgcgctcgtt ccggacgcgg ttgtgctgga catggtgttc gatcggcttg ttcagccgga
    11221 ttgcgcggcg gggtatctgc ttgacgggtt tccgcgcacg atcccgcagg ccgaagcgct
    11281 cacgaagtgg ctttctgaga aaaacgcggc gattgaccac gtggtactga tcgacgtgga
    11341 cgacgaggtg atagtggatc ggctctcgtc gcggcggacg tgtgagtcgt gcggagcgat
    11401 cgtcagcgtg aagttctcgc ctcctaagaa ggaaggcgtg tgtgatctgt gcggcgggaa
    11461 acttgtccag cgcgcggatg acaaccccgg gacggttcgc gatcgacttc aggtgtacca
    11521 caagaacacc gagccgttaa tcgcgtacta ccgtcagcag gggctgttgc gtgcggtaga
    11581 tggttcggcg tcaccggaac tggtgacgaa ggcgattctt gccgccgttg gtgcgaaatc
    11641 ctagctgaga gtggtgtctg tgccaaaaga agactcaatt gaggtcgaag gaaccgtaac
    11701 gaaggcactg ccaaacgctt cgttcaaggt gcgtttggaa aacgggcacg aggtctcggc
    11761 acacatatcc ggcaagatga gggtgaattt catcaccatc aaaccgggcg acaaggtgaa
    11821 gctcgagctt tcgccgtacg atctgacgcg cgggcgcatc acgtaccggc atcgctagac
    11881 gtgatttatg gagtgagttc gaatgaaagt gcgttcttcg gtcaaaaagc tttgcgccaa
    11941 ctgcaagatc atccggcgcc ggggtgttgt gcgggtgatc tgcaaatcga gtccgacgca
    12001 caaacagcgt cagggttgat cagagttgtc gtgcatcatt taacgtgagt gcaaagggag
    12061 tatctcaatg gcgcgtatag ctggagttga cctgccgcgt gagaaacggg tggatatcgc
    12121 ccttacctac atttacggga taggtccggc ctcatcggca aagatccttc aggaaacggg
    12181 cgtggatccg agccgcagga tgaatcagtt gaccgaagac gagatagccc gccttcgctc
    12241 ggtgatcgag aaccagttcc gagttgaggg cgccctgcgg agtgagatcg agatgaacat
    12301 ccgcaggttg caggagatcg ggtgctatcg aggtcttcgg caccggcgcg ggttgccggt
    12361 gagaggccag cgcacgcaca caaatgcccg cacccgcaag gggccgcgac gggcggtggc
    12421 cggagctcgc aaggccggga agaagacgaa gtaactggcg tccgccgccg tctgagcggc
    12481 gttgatcggg aaggttttga gaggattact gaatggcatc tccgactggt cgtgcccgga
    12541 agggacggaa aaaggaccgc aaagtcgaag cgactggcgt agcgcacgtg aaggcgacgt
    12601 tcaacaacac gcaggtaaca ctgaccgaca aggatggcaa tctgatctcc tggtcttcgg
    12661 ctggcaaagc cggtttcaag ggatcgcgga agagcacgcc atttgcagcg caatcggcgg
    12721 catcacaggc cgcccgcgaa gcgatggatc tgggattgcg gaaagtggaa gttcttgtca
    12781 agggacccgg cgtgggacgt gaggcggcgg tacgcgcgct tcaggcggct ggtctggaga
    12841 tcagcgctat tcgtgatgtt accccgattc cgcacaatgg ttgccgcccg ccgaaacgtc
    12901 ggcgcgtgta ggttgttgag aattcaggag acgtgaatgg cacgatatga tggtccggtc
    12961 tgcaggcttt gccgccgcga gggaacgaag ctgttcctga agggcgagcg gtgtaattcg
    13021 gagaaatgct ccctcgaccg gcggaattat gctccgggcc agcatggcca ggggacgcag
    13081 cgcaaggtga tggaatacgg ccggcagctt cgagagaagc agaaagcgcg tcggacgtac
    13141 ggcgttctcg agcgtcagtt ccggcggtac tacgagatgg ccgttcgaat acccggcgtt
    13201 acgggtgaaa acctgctgcg cttgcttgag cagcggctgg acaacgttgt gtaccggctt
    13261 gggtttgccc cgtcccgcgc ggcggcgcgt cagttggtgc gccaccggca tatcgaggtg
    13321 aacgggcggc cggtttcgat tccctcgttc ctcgtgcgtc cgggaatgac ggtttccgtg
    13381 cggcagggga gcaaagagat gcccagcatt cacgagtcgt tgaggcaact cgggaaatcg
    13441 cgcgagatgt cgtggctggt ggtggataag gtggcgcttt cgggacagat ggttcagctt
    13501 ccgtcccgcg aggacatcca gattccgttg gaagagcgcc tcattgtcga gttgtactcg
    13561 aagtaagccc ctcgtgcctg aagtgggaaa tgcccgcgca ggctctgaaa tgttccacga
    13621 gcggcagata ccgggtgttc tgcgggaata cgcgggtggt cgttcgtttc tgaggagggg
    13681 gttgtaggca ccatgatgaa aactgacaca gcagaaacgc cttcattcgt catgcccgat
    13741 gcggtggagg tcgatcaggc ctctacgcag acgtatgcga agtttgtgct ccaacctctg
    13801 gagcgcggct tcggcaccac gctcggctcg agtcttcgcc ggacgttgct ttcctcgatg
    13861 gaaggggccg cgattctggc ggtgcgtatt gagggtgtcc aacatgaatt tacggtgatt
    13921 ccgggtgtgg tcgaggatgt cccggaactc gttctgaatc tgaagcaggt acgtcttcgc
    13981 ctgcacacgg atgagaagaa gaccatccat gtgcgaaaaa agggccccgg ggaactggtt
    14041 gccggcgacc ttgcggtgga cagtgcagtt gagatcgtga atcccaagct ggttctggcc
    14101 acactgtcgg aaggcgcaga aatcgccatg gaagtggacc tcgggaaggg cagagggttc
    14161 cttctggcgg aggaaatgga atcggaagac attccgatcg ggacaatacc gattgatgcg
    14221 gtgttctcgc cgatcaccaa ggtggatttc gaggtaggaa atgcgcgtat cgggcagaaa
    14281 accgattacg acaagttgac gctggaagtc tggacggacg gcacgactac cccgcggagc
    14341 gcggttgcgt ttgccgcccg gcttttgagg gagcacctcc agcttttcat gggtgaatcg
    14401 gagcgtgagg aaacaggagc acggggtgtt ctggccgcag cgcccaccag cgttcttgat
    14461 acccgcgtgg aggacatgga tctttccatg cggtctatca actgcctgcg cgccgccggc
    14521 atcaccacgg ttcaggaact cgtcctgaaa tcagaaaacg atatgttgaa ataccgcaat
    14581 ttcgggcgga agtcgttgat cgaacttacc gagaagctcg agagcctggg gcttcgattt
    14641 ggcatgtctc ctgaagagct tgccgaggtg cgcgcccaac tttctggcgg ttcggcgcct
    14701 gaagagaaga gcgaggaagc gtaatcatga gacatgggaa caagggtcgc cgcctgggcg
    14761 taaccacgcc gcatcgccga tccatgatgg cgaatctggc agtatccctc attcagcacg
    14821 ggggcatcaa taccacagag gcacgtgcga aggaactgcg tcctttcgtg gaagagcttg
    14881 taacgctcgc caaacggggt gatctccatg cgcggcgcct ggttaccgcg cgtctgcgcg
    14941 acccgggagc gacgaagaag cttttcgacg agtgggctcc gaagttcgtc tctcacccca
    15001 cgaaagcggg tgacggatgg aacggcgggt atgtccgcat tctgaggctt ggcctccgga
    15061 agggtgactc tgcgtccatc tcgctggtgc agttcgtcac tgcggaatct gctgagtctt
    15121 cggcgagccg gaaaccggca caggagaaga aatcagagcc tgcgaagagc agctctgaaa
    15181 aggacaatgc ggcgagcgag gaaacgtcct agcgccgcac atcacacatc gggcattgag
    15241 aacgcgggac gtcgtcggca ccacgctgat gcgtcccgtt tcttttgatg cggacaggct
    15301 gtgagcgata ctacgacaca cgatagcggc atggcggctt ccgcgacgaa cggcgcagtg
    15361 cgccccggaa tgattgttcc tgcgattatc gaggggattg cgcccgatgg ccgtggcatg
    15421 gcgtccgcat cggggcggca gttgcttgtt cgcggggcac tgccgggcga tgaggtggat
    15481 gcacgggtgt tccgcaggcg cggaggacgt ctggaggcga cgtgcgtgtc ggtccgccgg
    15541 gaaggatacc cgcggcggga accacggtgc gcacacaccg agcagtgcgg gggatgtacg
    15601 tggcagcagt gggagcccgg cattcagcac gagatgaagc gacggatagt aagggaagcc
    15661 ctcatgcggg cgatgcccga tgcagccatc gaagtgttgc ctgtcgttgc ggcgggtccg
    15721 gaattcggct atcggaacaa gatggagttc acgttcggga aggatgaaac cggcgcggtg
    15781 actctcgggt tgcaccgtgt ggggcgattt gatgctacgt tcgacgtgaa gcgctgctgg
    15841 attgcgcccg aagggatgaa cgcagttcgc gcgtgggtgt gcgagtgggc gcggcgaaat
    15901 gcgctggttc cgtatgaccc gcggaggttc gccggcgcac ttcggcacct ggtgctgcga
    15961 gaggcgtcgg caaccgggga agtgatggcg aatctggtgt gcgcgacgga agaggtggcg
    16021 ggtctggatg aattgagtga agggcttccc agggaattgc cgcaggtaaa aagcctgctc
    16081 ttcagtgtca acacacggcg gggtgacacc gcgcatgcgg agacgacggt tctgctttcc
    16141 ggtcgtccgg cgatttcgga gaggatcggc gatatggaag tgacggtgtc gccgatctcc
    16201 ttccttcaga cgaacagcgc cggcgcggaa cggttgtact cggtcgtgag tgaagtggct
    16261 gcgctttcgg gcgaggagcg ggttcttgac atgtattgcg gcatcgggct tatcggcatt
    16321 tcgctggccc cgcgtgcccg ggaggtcgtc ggtctggagc aggtgccttc cgcggtacag
    16381 gacgcgaacg caatgcgaga acggctcgga tatacgcatg tgcagttccg tcagggagat
    16441 gcggaagcag ttctgcctgc ctgggcggat cgtggcgagc gatttgacgt ggtgatcgtg
    16501 gatccgccgc gtgccgggtt gcacccgaag gcgcttgcag cgcttgttcg tatgtctccc
    16561 cgcttaattg tatatgtttc gtgcaacccg gtgacactgg cggcgaattt ggctgatctg
    16621 tcctcttcgg gataccggat cggtgcggct catccggtgg acctgtttcc ccatacgccg
    16681 cacgtagaga cggtcgtgcg gctcgaaaag taagctccgc gcagaaagga tgcggcatgg
    16741 aatacgtcgt tgaaccggaa aaacgtgtac cggtggtgta tgacgtcgat gtctgcgtcg
    16801 ccggtgctgg cgtgagtggg gtttttgccg cgatcgccgc ggcgcgtaac ggtgcgcgaa
    16861 cggtggtggt agacagattc gggagcgttg gcgggaatgt cgggcccggc atgatcgtca
    16921 acggccagat gctgcccgga tcgccacacc cggacgttcc cttcacgaaa acggtctacc
    16981 ccggtctgtt cgggctggcg gaagaattca tcaaccgtta tgcggccctc gggggcggat
    17041 tgctgcagcc gtttacgaaa ggcgccaatt tctcgcgagg agccagcgtt gcttcgtatc
    17101 tgctgcagga aatgctggag gaaagcggcg cgtcgattct cctcccgacc tttgtggcag
    17161 atccgatcaa agaaggcgac cgggtgtgtg gagttttcgt ggaaaacaaa tcaggccgtg
    17221 cggcagttcg ggcaaaggtc gtgatagacg ccaccgggga ggcggacgtt gcgcggcggg
    17281 ccggagcgcc gatactactt ccgaaaaacg agtacaccgc gattgacggg cacgcaccca
    17341 cgggcatggg cacgtactac gtggtcgggg gaatcgcctg ggaccgtttc cggtctgccc
    17401 agaagggcaa cgaagccacg gaagacgaca ttgcctgggg aaccaagaca atcggggcgg
    17461 cgcttacgaa ggctcattcg tatgtccttc cttacctgcg aaaggccgtg gaggccggcg
    17521 agtatgaacc cgtggcgaag gtgatgatca acgggaagtc tatctcggtc caggggggcg
    17581 gcgtcgggca gtttgagggc aacgagctgg ggcacggaca ggttcaggtg gaacggacgg
    17641 aagaggtcga cgcgggcgac ggtctccaca tttcggccct tggagcggcc gtgcaggcat
    17701 acgcgttcga aaaggttcag ttcttcagca gatacgtgcc cgggttcgaa aacgcctatc
    17761 tcatcaccgt ggccccgttc ctgggatcgc ggggtgggcc gtgcatcgag ggggaataca
    17821 ccctcacgat ggaagactgc gtcgccggcc ggcgcttcga tgacgtgatt tatctgtacg
    17881 gcgagttccg ggcgctgagg aagacggcag aagccggggc gccgaaatgg gtggatgtgc
    17941 cgtaccgcgt gcttgttcca cagggcgtgg acggcctgct cgcgaccggg agatccgcgt
    18001 cgggcattcc ggatacgttg ctgcgaaacc ggcttgcggt gaaagtgatg ggcgaggcat
    18061 gcggaatcgc ggcctcaatg tgtgcggcca agggtgtcga accacgaaag ctcgaagtcc
    18121 gcgagcttca gaacgcactg ctggatgcgg ggttctacct cggggatcgc gcacgcttga
    18181 aagagctttc gttgacctga ttccgtggac aagaggaaag tctcacaaag ggagcacaaa
    18241 gacatgagaa catacgttgg tgtggtggtt tcagggctgg cccttctggc aagttcctgc
    18301 gcgaagcagg gcccggcggt ggtgccggtg caacgtgtgg aaatggttgc cgccgcggat
    18361 ctgccggtcc ttggaacgcg ggcacttccc accgatccgc agaagttcag cttcgtcatc
    18421 ctgggcgaca aatccggagg actggaccgg aactggccgc ttttcgaccg ggccgtacag
    18481 gagattaatc tcctgaagcc cgacttcatc atcacggttg gagaccagat cgaaggatcg
    18541 aacgacagcg ccagcgtggt ggcgcagtgg gaacttttca gaagccacgc gggaaagttc
    18601 gaggcgcctc tgttcatgct tccgggcaac cacgatgcag ccgacctgat ggggtacgag
    18661 tactggaagc gtaacgtggg tcggacctat tacgacttca cgtataaagg atgttacttt
    18721 ctcgtgctga acacgcagga agcttcccgc gaattccctc agttcggcga ggcgcagatc
    18781 gcgtgggcgg tggacgcgct ccagaggcac cccaacccgc ggcacacgtt tgtgttcatg
    18841 cattatccgg cgtggttcgc gagtgatccg gagcggcagc ggggtgggat ttccgcggaa
    18901 tggcagcgaa tcgaaacggc gctgggaaac aggaaataca gcgtcatcgc ggggcacacg
    18961 cacaacctca tgtgggcaac gcgtgacgga aatcgttacc tcgtgcacgg cgccaccgga
    19021 gccacgctca cgccgagtcc ggtgaagcag gtcggtgcgt ttcaccatta cgccaaagtc
    19081 actgtggagg cggatcaggc gcacatcgcg attgttgaac ccggaaacat ctggccggag
    19141 acgattgcgc cgctcgaatt ccagcggaac atcgggcgct ttgtgcgggt tcagtcgagc
    19201 aggcgagacc tcgccgacgg acggatcgcg atggacgtta cggcggagct gaacaaccat
    19261 gtgggcgaca cggtgtccgt agcgttgata cccgtcctcg gggccgcggg agtctgggct
    19321 ccttctgcgg acagcctcgt ttcggtcctg ccggacggcg gaacggacag cctgacgctt
    19381 acgtttgtcg ggagccgcga caactggtat ccaacgccgg cgtggaagat ggtagtccgc
    19441 tatcagggga aggaggtcga cacctacgga ccgtcgcccc ttaacccgtt ttctgagagc
    19501 cgcgccacgg tactaccgga atggcgtgcg ctgggcacgt ttccggtggg ccccatcaac
    19561 cgcgatgtca tgccgggcaa tcctcgggcg ggcgtacccg ggatgtttgt accgcgcgca
    19621 cctgatgcag ggtggaacgg cggagctccc atcacggtgg acggcaggac gtattcgtgg
    19681 gttaccgcac ggccggattc gaccggacgc gtaagcctgg acagagtatt cggtatcgtg
    19741 gatctgtcgc tggcgtatct gtcaaccgct atttacagcc ccgttgatca acgggtaccc
    19801 atccagatcg gcgtggacaa tttcttccag ctcttcctca atggccagat ggttccgggt
    19861 ggggaggcat acgggatgcc ctacgaacgg aaaatcctgc ctctcgacct gaaagcgggc
    19921 tggaactcac tctacctgaa ggttgtgaat aatcgcggca actggggcgt tgaagcatcc
    19981 gtgatcgacc tgcggggcaa tctgcgtttt gcaccctatc cggggcagaa ctgagacaag
    20041 ggtttttcgc gtcattgcgg cgtatcggga cgatagacac aaaggtgagg gacacaatgg
    20101 gtgggagtgg aacagaccgg ctatccggtt ggaggcgtac gcacaggtgc ggggatttga
    20161 gggcttctga cataggattg gacgtcatct tgatggggtg ggcgagttcc catcgagacc
    20221 atggcggcgt catcttcgtg gacctgcgcg accgttgggg tatcacccag gttgtcttcg
    20281 atccggcggt gagcaaggaa gtgcacaaac gggcggaaca catccgccag gaatacgtga
    20341 ttgccgtaaa ggggcgggtt cgtgcgcgca tggagggaat ggcgaacgcg aaacttgcga
    20401 ccggggaaat cgaggtcatt tgcaacgaac tgcgggttct caatagtgcg gaaaccccgc
    20461 ctatccaggt ggatggcccc cccgcgaacg aggaaattcg cctgaagtac cggtatctgg
    20521 accttcggcg ccccatcatg cagaggaacc ttgagttgcg ccacgccgcg gcgcaggcca
    20581 cacgcaggtt cctgtgcgcg caggatttcg tggagatcga aacgcccacg ttcgtgagca
    20641 gcacgccgga aggagcgcgg gattatcttg ttccgagccg cgtgtggccg ggcaggttct
    20701 acgcgcttcc ccagtctcca cagatctaca agcagctttt gatggttgcc gggatggatc
    20761 ggtatttcca gattgtccgg tgttatcgcg atgaggacct gcgcgcggat cgccagcccg
    20821 agttcacgca gatcgacgtg gaaatgtcgt tcgtggaaga ggcggacgtc atgagggtgg
    20881 ccgagggcat cacgaggtcg gtgtacaaag aaacgatcgg gtatgacgtt ccggactcgt
    20941 ttccccattt ggattacgcc gatgccatgt cgcgttacgg gtcggataag ccggacatcc
    21001 gtttcggcct tgagatggtt gatgttaccg acatcgcggc ggagtcggat ttcaaggtgt
    21061 tccgggcagt tacggaagcg ggcggaattg tcagtgcgat caacgtaaaa ggcggggcct
    21121 cgttcagccg gaaacaggtt gacgacatga ccccgtttgt cgcgcagtac ggggcaaaag
    21181 gcgcggcctg gatgaaggtt gccgaacggg gtctggaatc caatatcgcg aagttcttca
    21241 atgaaggcgt gcagaacgcg ctcagggaac ggctcagcgc cgaacagggt gacctgctgg
    21301 tgttcgttgc cgaccaggcg ccgattgtgt atcaggcgct gggcaacctg cgcttgaagt
    21361 tcggcaatga cctcaacctg atcgacaaat cgaagtttgc gttcctgtgg gtgacaaact
    21421 tccctctcct ctcgtgggac gaggaagaaa accggtgggt ggcaaaccat cacccgttca
    21481 cgtcccccgt tctggaggat ctggaccttt tcgtgtcggg tggcgatccc gggcgaatcc
    21541 gtgcccgggc gtacgacctc gtgatcaacg gcaccgaagt gggtggcgga agcatccgca
    21601 tccaccagcg gcacgtgcag cagcagatgt ttgaccttct gggcatctcc cccgaggaag
    21661 ccgaaaggcg cttcgggtac ctgctcaacg cgtttcggta cggggcgcct ccgcacggtg
    21721 gtatcgcctt cgggtttgac cgcatggtga tgcagcttgc cgggcaggag tccatcaggg
    21781 aggtgatcgc gtttccgaag acgaacaccg cggcttcacc tatggacggg agcccttcag
    21841 aagtggacga gcggcaattg cgcgagctgg gtctcaggct tcggcacgtg aagcatcaag
    21901 gaactgcata acgccgcgtt gcgaagtcgt atgccacggg aggcgcggcc acaatggtcg
    21961 cgcctcccgt tttgtatcgg atccgataca cggagaaggg gagcacccgg gggaaagacg
    22021 agcaggcacc aaagcggcgt ccgcgcgtac agagattcgt gtacgattgc ggcacgaaaa
    22081 ccgtatattt tgcctagatg agcaggaata tgacgggaat gcgaaatgcg gcgcgattgt
    22141 tgctctgata cagatgaaca gacacgcaga atatttcccg cgtgaacgtc ttattcgggt
    22201 acgaaccggt atagcgcgaa gggctatcgg aacaacggta gttctggttt gaaggagaac
    22261 aggcatgtcg aatcgcagaa gggaatttcg cgggcagatt ttcgccctca tgatgtttct
    22321 gggcgtcagc ggcgttgcgt ccgcacagac cacaacgatg ccgaacgtgg tgggtatgga
    22381 tatagcctcg gccacggcgg cggtgaaggg taccatcgcg cggtttgcga cgctgtacgt
    22441 cgagccgacg gatgacccgg ccgccgttgg caaaattgac ggccagtacc cgaccgcggg
    22501 atcggaactc tcgtcatcga ctgtggtgac actcacggca cggcgggcga acacacccgg
    22561 tttgctgacg ctcgcatccc cggcgaaggg tgccacggac gtttcggtgg ga
//
LOCUS       DAOTNG010000009        22583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2355_length_22583_cov_5.564409, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000009 DAOTNG010000000
VERSION     DAOTNG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 22583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22583
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_22583_cov_5.564409"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="P5179_01005"
     CDS             <1..820
                     /locus_tag="P5179_01005"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRS93832.1"
                     /translation="KLLIVATDRISAFDCVLPTPVPQKGVILTQMSLFWLSMMSDILP
                     NHLITANVAEYPADLRKYGDQLEGRSMLVRKARRIDVECVVRGYLAGSGWKDYQKTGM
                     VCGHALPPGLRECDQLPEPIFTPATKEDSGHDVNVSREHLVTLVGNELASELERLSLA
                     IYERGREYAEKRGIIIADTKFEFGWIDGRISLIDEIMSPDSSRFWPADTYQPGRAQDS
                     FDKQYVRDYLETLDWDKTPPAPPLPADILAGTQAKYREAYERLVGKPFRPFEGA"
     gene            830..2473
                     /gene="purH"
                     /locus_tag="P5179_01010"
     CDS             830..2473
                     /gene="purH"
                     /locus_tag="P5179_01010"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRS93833.1"
                     /translation="MVKRALISVSDKTGIVPFAEALVSELGVEILSTGGTARALREKG
                     IAVTDVSSFTGSPEILDGRVKTLHPAVHGGILCRRDHPDDRKLIENGSIRPIDLVVVN
                     LYPFRETVAKPDVTIEDAIENIDIGGPTMIRSSAKNHAWVAVITDPKDYDSVLEELRK
                     TGGEISEKSRRRLAVKAFRHTAQYDAAIDTFLSERLEGEHVFHGHYVDGRVLRYGENP
                     HQTPAYVYRNPASDEPSVVSGKLLHGKEMSYNNYVDANSALAMVKDLADSIAVSVIKH
                     NNPAGIATGETLAEALERAWYGDPLRTTPMGSVIAVTRPVDLAAAEFLKGRFVEVMVA
                     PGFEPDALEFLRGKSKDIRLVEIAPLATGTPEPFMAKTIAGGLLVQPVDRQVTATWNV
                     PTTAKFPPSLEPLAHFAVIAVKHTRSNAIILAREYRPGFYELVGLGAGQPNRVDSLRA
                     LAAPKARANFEMEYDEKRALNVKAGETRDEYVARQFSNVVLASDAFFPFDDSAVAAHS
                     WGIRYLVQPGGSIRDDEVVATCDKLGIAMALTGMRHFTH"
     gene            2531..3550
                     /locus_tag="P5179_01015"
     CDS             2531..3550
                     /locus_tag="P5179_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRS93834.1"
                     /translation="MRFSLAGMISNDIGVDLGTANTLVWVKGRGIVLNEPSIVAVERN
                     TRKPLAIGAEAKQMLGRAPSEIEVIRPMKDGVIANFEIAEEMLRGFLTKVQKNRGLFR
                     PRVVICVPSGITEVEKRAVRDSAEAARANEVYLIAEPMAAAIGAGLPIENAVGSMIID
                     IGGGTSEIAVISLSGIVTTISERIGGDEMDEAIIQYVKRTYNLSIGNRMAEEIKIKIG
                     SAYPLPEEMEHTVRGRHLVTNIPQVVVVTASEIREALSESIDRIVQAVLQALERTPPE
                     LAADIIDRGIALSGGGALIKGLDERLREATNLPINVVDDPLTAVVRGTGTVLEDISAY
                     KKVLM"
     gene            3601..4440
                     /gene="mreC"
                     /locus_tag="P5179_01020"
     CDS             3601..4440
                     /gene="mreC"
                     /locus_tag="P5179_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRS93835.1"
                     /translation="MLQAFTHWVWAHIRGVTLTLTVVLSTALLIRGPQASIGPLRWGI
                     VHVIAAGQRVFGLPIELAHLRDENRYLRSLVLSHIIHQAENRELRSENERLRQLLNFR
                     ERSRFSLIAARVLAHDSEVPPTTITIDVGFVDGVGANMPVMTPEGLLGRVEGRPAGHR
                     SLVRLISNPSLRVAAVVENEDRGMGILRCDGKRLRVENVAQEARVREGDPVVTSGMGG
                     IFPPGIYIGKVLKVRNDPHALFKEMLISPGARLDRLEEVFVVRETSPSAAIIGGVPDA
                     VDR"
     gene            4424..4924
                     /gene="mreD"
                     /locus_tag="P5179_01025"
     CDS             4424..4924
                     /gene="mreD"
                     /locus_tag="P5179_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRS93836.1"
                     /translation="MRWIVEALWLAAGAIAYATLGQEIALLGAVPILPLITIVRIAIT
                     SDELAGNLSGFGAGLLLDLFSLEWFGASMLVGSAVGYTLGVARKHLVIGNLATRGIIL
                     LVSAEVFALGVVLIKSARGAPGPEPFITALGSGIYTALVGIAWWFCVDLARGIFGWRG
                     LWDGAR"
     gene            4911..6740
                     /gene="mrdA"
                     /locus_tag="P5179_01030"
     CDS             4911..6740
                     /gene="mrdA"
                     /locus_tag="P5179_01030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRS93837.1"
                     /translation="MVRADLSGMRSARIKVLMAVVLIAFLAITVQLYTLQVLRHDTLR
                     RRSEQNAMRTVPVEAIRGRITDRNGVVLVGSRPAYTVGVIPAEVKDAAHLDSMVCPIL
                     QIPPGTVSGKVSQQRQSLHRPIELRRDAPFQIIAYLEEHRHELPGIVYFVESRRRYPF
                     GILAAHLLGYVREVRESQLKQLKDEGYALGDLMGQAGVEATYERELRGEKGEEYQEVT
                     ATGRVVDSHRREPVRGMDARLSIDLALQQVAESAMDSIRRGALVALDPRTGEILALVS
                     RPAFNPDIFSTVVPPEIWRELNDDVERPLFNRATMGTYPPGSTAKMVSAVAGLETGVL
                     DSSTRLRPCTGGLAFGNRVYACWNKRGHGSLNVVHAIEASCNVFFYQVGERVGVDKWG
                     TYARLLGLGQKTGIDLPGEDEGVVPSTQVYDRRFGKGKWGRGEALNVGIGQGIVLVTP
                     VQMARYAAALATGYLVTPHLAIGFTDTNGVEHAPALRPPKPVSIQPDVLRLIRSAMVL
                     VTEGGSGTAHRAAVPGFHVAGKTGTAQNPHGDDHSWFVGFAPVPNPTIAIAVICENAG
                     HGSTRAAPVAGAVLRRHLLGPEQPRPKKDEEMTAGHPIAQLRN"
     gene            6746..7978
                     /gene="rodA"
                     /locus_tag="P5179_01035"
     CDS             6746..7978
                     /gene="rodA"
                     /locus_tag="P5179_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRS93838.1"
                     /translation="MATSRQQLRKYLDWPLVGAALGLVACGIVGLLSATTGTATARVS
                     GLAGIQFLWLVLGIGVAIGAIRVPTRFWFGISYILYGISIIALVLTLAVGVSRMGAER
                     WLAIGPFRFQTSEMGKIGLILALARYLSFHKVSVSRFRDFAVTVGLVLVPIALIHRQP
                     DLGTALVYGSIFLPMVYWAGLANLHLLAIITPVINAVCAFAGWIPWLVFLLILVGVLF
                     LMRPPLRWTVVIAVINLVVGIVTPAIWDRLEPFQQRRIETFFSPEKDPLGAGYQIIQS
                     QVAIGSGGLWGKGFLEGSQTRLAYLPETHTDFIFAVLGEEFGFVGAIVIVALYVLLIW
                     RIFRMALVANNNFASFVCIGVGSMFAFHGIINIGMTIGLAPVTGLPLLLVSYGGSSLL
                     TNGLAIGMVLGFGVRRFE"
     gene            8001..8978
                     /locus_tag="P5179_01040"
     CDS             8001..8978
                     /locus_tag="P5179_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93839.1"
                     /translation="MKSGVRRLSLLFVVLPLGAVLMWSAWVVAAAGSSLRIFYTASVK
                     GYIDQCGCRYNPTGGIGRRATFIQRNTDGAIPMILLDGGDVIGEESPVERMQTTYLFR
                     AMKEMGYRTIGVGPRDFAYGIAYLREAERTYGFTFTCANLVDDATGRTLFAPHVVERV
                     SGKGGFRRNAATVRVGVVNVMGQDRLPLTVPSDPILRVTPPVPAVQNAVATLRPQTDV
                     IVVMVYGTQQLLDSVRAVPGVGAVLATRPLRLPDQWVSFNRTNVVGYSSFQGRGVGKI
                     DISLAGPHRITSGRGEMAMMTADIPDQPRMAELKREFEAWKARNAGGDR"
     gene            8945..9985
                     /locus_tag="P5179_01045"
     CDS             8945..9985
                     /locus_tag="P5179_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93840.1"
                     /translation="MEGTQRRGRQVAVRRVPGRWWAVFGVIAAIVVFFATLSLPQDAS
                     RLFIFFTATAHANLEPCGCPVNPSGGVSRRAAFIRSHTPPDTPVLLLDGGDVVGPPTP
                     EGLIRSEFMFRAMEEMGYSVLGLGARDFSFGPDFLRFAEKYYGFTFVGTNLLSSRTHA
                     HLFAPYAIARVGNGRILGIPFGGLRVGVLSVMGRDLIPFIPDTCEPAFLLDPLAAVRT
                     FSRRIRSQCDLVVVLAYTDQALLDSLVSMPGVDIVIAARTLNVPPGYTNVGMRGRAAL
                     AYTGYEARRIGVMRIRLDSSRNMTEARGDLITLSENLPEDETVRAMVQESRRAVAALP
                     NPGSLQKEPPAQ"
     gene            10021..11187
                     /locus_tag="P5179_01050"
     CDS             10021..11187
                     /locus_tag="P5179_01050"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS93841.1"
                     /translation="MSTSSHGRLVALSVVLFTVFGAALTLTGTLLPQLIEHFGLSYGE
                     AGTLQVSSQVGYLAAIVLSGWLSAKIDGRMMLLAAIVFSLIGYGAFSLAPSFPAACAL
                     MIVGGAGGGAIEVIINAVVMKLSETRRSNLLNFIHLFFGVGAFIGPAVVGKLLALGVT
                     WNWFYFGTAVCWTALLLGTLACRFPSIKPVETASSEPARSVFSLPLARVLFLVLTLYV
                     GLELGVGGWVTTYLVHHEGMAPSYAAYSASAFWLGIAGGRLILGVIGHRWHESGILTI
                     LAAFSAACLLAAVLVPSPFVAALFLALTGLGYSGIYPTAVSFGGGAYPEQSTPVTALL
                     VTGGGIGAVAFPWLMGHISDGFGLRGGMLFYIVLNILLLGFILTARRMHSRAQT"
     gene            11206..11625
                     /locus_tag="P5179_01055"
     CDS             11206..11625
                     /locus_tag="P5179_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRS93842.1"
                     /translation="MKSFRKELWFNIPSRRGFVNITKDVEQCVAESGVTEGLCLVNAM
                     HITASVFINDDEPGLHGDYEEWLEQIAPHEPISRYRHNRTGEDNGDAHLKRQIMGRET
                     VIAISRGKLDFGPWEQVFYGEFDGRRKKRVLVKIIGE"
     gene            11649..12419
                     /locus_tag="P5179_01060"
     CDS             11649..12419
                     /locus_tag="P5179_01060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HRS93843.1"
                     /translation="MSTLDLFRLDGKVAIVTGAGRGLGREMAIALAEAGADVAITSRE
                     MSHVEPVAEEIGKLSGRRVLPVVADVTRPDDVQRMVDTTCRELGGLDILVNNAGVNIR
                     HLAHEYTMEEWNTIITTNVTGVFLCCQAVLPKMMAQKSGRIINIGSMMGVVGMPLRVP
                     YSASKGAVHQMTRTLSLEVAKYGITVNAIAPGPFLTEMNRQVIDQPDVYNFFVERIPL
                     GRWAEPNELRGVVVFLASQASNYITGSVIMVDGGWTAQ"
     gene            12485..13477
                     /locus_tag="P5179_01065"
     CDS             12485..13477
                     /locus_tag="P5179_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS93844.1"
                     /translation="MELRQLGKSDLKVTPVVFGAWAIGGWFWGGTDDAEAVTAIRAAV
                     DEGINTIDTAPMYGFGHSEEVVGKALKGIRDKVIVATKCGLRWNRTDGDFHFVAQDEQ
                     NRSHQIYKVLKPDSIIEECDLSLKRLNVDVIDLYQCHWPDTTTPIADTMGALMKLKEQ
                     GKIRAVGVSNFTPAMMRECLNAAPLASDQPKYSLLAREIEADVLPFCRENNIGVIVHS
                     PLAQGLLTGKVTMDRQFAPGDLRVNTPWFQPQNRQRVLDALGRIRPIADAHRATLGQV
                     AINWVINTPGVTSAIVGARNAAQVRENVHAAEFRLSEEEQSFIRSTFESLGKPV"
     gene            complement(13553..13852)
                     /locus_tag="P5179_01070"
     CDS             complement(13553..13852)
                     /locus_tag="P5179_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93845.1"
                     /translation="MVRSLLVTTLGLTGLVAATFPYQGISPARSGKVAAETEFQSGIV
                     LAEPAGDERHLPDLFVVASRENDGQFALTNAGKGADLPDLVVFGTRARSEDQPSS"
     gene            complement(14106..14465)
                     /locus_tag="P5179_01075"
     CDS             complement(14106..14465)
                     /locus_tag="P5179_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93846.1"
                     /translation="MKLHLIVLTVAAAALSIGALPAPKTTQSRTDSAPVETTLPDLLV
                     TAERTIAGGATGAVTKASQSGDLPDVVVVAEGPLSTAAPLEFRIQGGDLPDVTVFADR
                     PARTSAIAADHTVVTSF"
     gene            complement(14578..15417)
                     /locus_tag="P5179_01080"
     CDS             complement(14578..15417)
                     /locus_tag="P5179_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRS93847.1"
                     /translation="MQDIRVAAVASLSRIGEIQTNLENIRQWSAKAKEQGAELVVFPE
                     LSVTGFWLHRDAHRYAQPVPGPATDTLVQIASEMGVLLVAGISEECQGRYYNTHVLVS
                     PSGILGTYRKTHINPYESPYYYEGNSLSVFDIGKARVGISICNDNLYPENLAVLALKG
                     AEILLMPFAYGGSNHNLWLASSACAAYRTRGLDLGCFVVAVNNAGPVPTPDGETNRYP
                     GGAFISDPEGEIVARTQGMGPGEKMLVADLRAKALHDRRSVPHFTLRLRRPELYGRVS
                     ELL"
     gene            complement(15433..17286)
                     /locus_tag="P5179_01085"
     CDS             complement(15433..17286)
                     /locus_tag="P5179_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS93848.1"
                     /translation="MSDSRTNQPVDLAEVLLRSADPFFFVDDAGIIRAWNFGAELLLG
                     WTRDEALGAEVSIVLPEIPPGPPSEEEPEESLHDSVVAENFQTVFKAKDGHQIPVKIT
                     VTLIRDENGVLLGSAVNARNVAREKALEQELRWRIGEMEMLDNVARSVQSTLDIQRVL
                     RIILTAFTAGSGLGFNRAILFLVSGSLLEARLGIGSSNWEEAGRMWPRVADKPDLQSV
                     IDFVLAEDDTTPSVVHEIARDWRIPLEEAENPLIICMREKRSLIWPDSGDPGGSVAEK
                     LRSPMFAAVPLLHGGRAIGAVLADNVVTGRPIDEHAVRLLRLMAGSAASAISNARLYE
                     EVTRHAQNLEKASEQIKEQQDLLLQSRHFASLGRIVAAINHEVQGPLVPIGGFARALR
                     REVVDGSPQAEKLDFIIKEVARLENVVKGVASLAAVPLPTLHPVLLSALVEKVYTVVR
                     ADAGARGVILESDIPPDVPPPHIDHDQWYQALLVLVSRAVEVTPDGGTVSTSLRAEHG
                     RYVIRVSDSGPGIPQNQISKAFGPFFSTQPSLEAAGLNVVAEIVRRHFGRVSVQSSAT
                     GTTVAVDFPMADELYRLVEKQRADESNEDRIGLLDPLTQEVLSSASLANGN"
     gene            complement(17324..17986)
                     /locus_tag="P5179_01090"
     CDS             complement(17324..17986)
                     /locus_tag="P5179_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRS93849.1"
                     /translation="MDTNLLLTGLWHYGVALLALFPIVDPITAAFSFAGLTQNREPAW
                     TNRQALLACIYMVVTLIAFQILGTWILRFFAITLEAIRIAGGVMIWYAALEMLRGTDR
                     LSADEQIEGVAKHDIAFTPMAMPLLSGPGAIAVTLTLSARSERFVNEVAVGVAIITIA
                     VVTYLALRFASALQKHISETSRLAFSKVLGFLLLCVGVQFIMSGVAPLLGSGAFNAPT
                     GP"
     gene            18284..21391
                     /locus_tag="P5179_01095"
     CDS             18284..21391
                     /locus_tag="P5179_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRS93850.1"
                     /translation="MLALGLDIGAISIKLALVADREDADLVRGLVDKNDQYFLLEGVP
                     EGKVIVLSGYRRLFGSPRQSAEKMIAEVATSIPVDRVSFVGLTGIGAYPIAKTAGLAS
                     INEFRAVAQAVGYLHPEVETIFEMGGENSKFIRLARDRQTGDVGIADYEKNGDCAAGT
                     GSFIDQQAARLKFSIEEVGGVVSGAGKPPTIAGRCSVFAKSDMIHAQQKGYQPPEILK
                     GLCEAVARNFKGAITKGKEIKPPIAFVGGVAANAGVVGAMRTVFELEEQDLFVPPAYA
                     WMTAIGAALLAPADEKGNRVEEFFRLRAKASGATHVLPTVQKLSMDRVISLRDRVKPY
                     TFEGKQLPVPVHLGIDIGSVSTNLVVIDDNGDLVYEIYTYTQSRPIQVVDAGLREIRD
                     AIGDKISVQSAGTTGSGRELIGLLIGADTINDEITAHKTGASYVGSRLINTKPDTIFE
                     IGGQDSKFISLNEGVVIDFTMNEACAAGTGSFLEEQAMKLGVQIKGEFARRALSAESP
                     TKMGERCTVFMEKDVTAYMQQGATVDEICAGLAYSVALNYLNRVVRGRPIGEHVFFQG
                     GTAYNDAVAAAFTQILDRPVIVPPHNGVIGAIGMALLAKDAVARKGERTTFRGWSLDA
                     INYELREFTCKGCSNHCDIQQFDVEGEKTYWGDKCSDRYRKSAKVNKKPVIEDLVARR
                     MEALLDGYHEPSVSGRVRVGIPRAMYFYDWFPFWKAYFAALDVDVVLSDTTSKQVINE
                     GTEAAVAEPCFPIKVAHGHVHNLLAKQVDFVWVPNMIDGETEFPETNSMLCPWGQTMP
                     FVFRAAPKFESLTGKWILPTLHPRRGEVVIERELWPVSQMFGASVGQHRRAVNAGYEA
                     SRRFRARLLALGQEALAILRAKDEMGIVLLGRSYNVNDPGTNLNVPTKLRTLYGTNVI
                     PMDCLPIVGIDIKDVNDNMYWNYGRKILQAARFVSQQPNLRVIYITNFKCGPDSYIKH
                     YTKDAAGGPFLTLQFDGHANDAGTLTRCEAYLDSQGFFTHEPRPIERPAQKALSRTRE
                     ERVEA"
     gene            21388..>22583
                     /locus_tag="P5179_01100"
     CDS             21388..>22583
                     /locus_tag="P5179_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93851.1"
                     /translation="MRSTGNPLAGRTLYIQQMTYASTHLLAAAFHSVGINAVVTEESD
                     ERTLELGGKFSSGEECLPEKITLGDYLKVVFSEGFDAKRAAFLMPTANGPCRFGQYSG
                     LLRKVLNDLGHHEALIFSPTSANGYDGFGANGNIFLRSAWRAVVVGDVLTKALLRTRP
                     YELRKGDTDEVFLRSIRRMGDVFSEKVESAGSQLERLVRALSVCHDEFCSLPVDMSEP
                     KPLVGVVGEIFCRLNDFSNDSIVRKLEEYGCETWLSDVAEWVWYTNDEAKTNMDADGL
                     LKIHPKRIKLWAKTKFQRHDEHALLSAFGDYFAGAEEASDVTEILNASRPYLPKEGSL
                     GEMVLSSGKAIYLYRMGADGIVDISPFTCMNGIVTEAIYTKLSKDCDGIPIRTFYFDG
                     ISSDLDR"
BASE COUNT         4576 a         6235 c         7119 g         4653 t
ORIGIN      
        1 aaaactcctc atcgtcgcca ccgacagaat cagcgcgttc gactgcgtcc tgccaacgcc
       61 ggttccgcag aaaggcgtca ttctgacgca gatgtcgtta ttctggcttt ccatgatgag
      121 cgacatactc ccgaaccacc tcatcaccgc aaacgtggcc gaataccccg cggatttgcg
      181 caaatacggg gaccaactgg aaggtcggtc catgctggtt cgcaaagcca gaaggattga
      241 cgtcgagtgc gtggttcgag gatatctcgc aggatcaggc tggaaagatt accagaaaac
      301 cggaatggtg tgcggacacg cattgcctcc gggtctgcgg gaatgtgatc agcttccgga
      361 accgatcttt acgccggcga caaaagaaga ttcaggacac gatgtgaacg tcagtcgcga
      421 gcacctcgtg acactcgtcg gaaacgagct ggcatcggaa cttgaacggc tgagccttgc
      481 gatctacgaa cgaggccggg aatatgccga aaaacgcggc atcatcatcg ccgatacgaa
      541 attcgaattc gggtggattg acgggcgtat cagcctgatt gacgagatca tgtcccccga
      601 ttcgtcccgt ttctggccgg cggataccta tcagccgggt cgggcgcagg acagcttcga
      661 caagcaatac gtgcgagact atctggaaac cctcgactgg gacaagacgc ctcctgcgcc
      721 accgctgccc gccgacatac ttgccggcac ccaggcaaaa taccgcgaag catacgagcg
      781 tcttgtagga aaaccatttc gtccttttga aggagcgtag cacatcgtga ttgtcaaacg
      841 tgcattgatc agcgtttcag acaagacagg catcgtgcct tttgctgaag cgctggtttc
      901 cgaactcggc gtcgaaatac tctccacggg cggcaccgcc agggccctcc gtgaaaaagg
      961 catcgcagta accgacgtct cttcgttcac cggcagcccg gagattctcg acggccgcgt
     1021 aaaaacgctt caccccgcgg tccacggagg cattctctgc cggagggacc atcccgacga
     1081 tcggaagctc atcgagaatg ggtccattcg cccgatagac ctggtggtcg taaacctcta
     1141 ccccttccgc gaaaccgtcg cgaaaccgga tgtcacgatt gaagatgcga tagaaaacat
     1201 tgacatcggc ggtccgacga tgatccgttc atcggcaaag aaccacgcat gggtcgccgt
     1261 gataaccgat ccgaaggatt acgattccgt attggaggaa cttcggaaaa ccgggggaga
     1321 gatttcggag aagtcgcgcc ggaggctggc ggtcaaagcg ttccgccaca ccgcgcagta
     1381 cgatgcggca atcgacacct tcctcagcga gcggctggaa ggggagcacg tcttccacgg
     1441 tcattacgtg gacggcagag tgcttcgata cggcgaaaat ccccatcaga cgcccgcgta
     1501 tgtctaccgt aaccccgcaa gcgacgaacc ttccgtggtt tccggaaaac ttctccacgg
     1561 caaggagatg tcctacaaca actacgtcga cgccaattcg gcgcttgcga tggtgaagga
     1621 tctcgcggat tccatcgccg tttccgtgat caagcacaac aacccggccg gtatcgccac
     1681 gggagaaacc ctcgccgagg ctctggaacg ggcatggtat ggcgacccgc tgcgcaccac
     1741 ccccatggga agcgtgattg cggtgacgcg accggtggat ctggccgcgg ccgaattcct
     1801 caagggccgg tttgtcgaag tgatggtggc accggggttt gagcctgatg ccctcgagtt
     1861 tctccgcggt aaaagcaagg atatccgcct tgtggaaatc gcgccgctcg ccacgggcac
     1921 gcccgaaccg ttcatggcaa aaaccatcgc cggagggttg ctcgtccaac ccgtggacag
     1981 gcaggtaacc gcaacgtgga acgtgccgac caccgcgaaa ttcccgccct cgctggagcc
     2041 actcgcccat ttcgcggtga ttgccgtcaa acacactcga tcaaatgcca tcatccttgc
     2101 gcgtgagtat cgccctgggt tctacgaact ggtgggactc ggagcgggcc agccgaaccg
     2161 tgtggacagt ctccgtgcgc tggcggcacc aaaagccagg gcgaatttcg agatggagta
     2221 cgacgaaaaa cgcgctctga atgtgaaagc aggcgaaacg cgggacgaat acgtcgcgcg
     2281 gcagttcagc aatgtcgttc tcgcgtcaga cgcgttcttt ccgtttgacg actcagcagt
     2341 tgccgcacat tcctggggaa tccgctatct tgttcagccg ggcgggtcca tccgcgatga
     2401 cgaagtcgtc gccacgtgtg acaaactggg tatcgcaatg gcgctgaccg gaatgcggca
     2461 ctttacgcac tgaaccaatt caacgaaacg ggacaacaca cacgccacag caccagcgtc
     2521 gaggagtgcc atgcgattct ctctcgccgg gatgatttca aacgacatcg gggttgatct
     2581 cggcactgcg aacacccttg tctgggtaaa aggccgcggc atagtgctca acgagccgtc
     2641 catcgttgcg gtcgagcgaa acaccaggaa gccgcttgcc atcggcgcgg aggccaagca
     2701 gatgctgggc cgtgcaccgt cggaaatcga agtgattcgt ccgatgaagg atggcgtcat
     2761 cgcgaatttc gagatcgccg aggaaatgct ccgcggcttc ctgacaaaag tgcagaagaa
     2821 tcgggggctt ttccggccac gggtggtgat ctgtgttccg tcgggtatca ccgaggttga
     2881 gaaacgtgcg gtgcgcgaca gcgccgaggc cgcccgggcg aatgaggtgt acctcattgc
     2941 cgagccgatg gccgccgcga tcggtgcggg gcttcccatc gagaacgccg taggctcgat
     3001 gatcatagat atcggcgggg gaacaagtga gatcgccgtg atcagccttt cgggtatcgt
     3061 gacgacgatt tccgagcgca ttggcggcga cgagatggac gaagcaatca tccagtacgt
     3121 gaagcgcacg tacaacctgt ccatcggcaa tcgcatggcg gaggagatca agatcaagat
     3181 cggctcggcg tatccactgc ccgaggaaat ggagcacacg gttcgcggcc gccatcttgt
     3241 aacgaatatc ccgcaggttg ttgtcgtgac ggcttccgag atccgggaag cgctttcgga
     3301 gagcattgat cggatcgtgc aggcggtgct tcaggctctt gaacgcactc cgccggaact
     3361 tgcggctgac atcatcgaca ggggaatagc cctgagcggc ggcggcgcgc tgatcaaggg
     3421 gctggacgaa cgtttgcgag aagccaccaa cctccccatc aacgtggttg acgacccgct
     3481 taccgcagtc gtgcggggga cgggcacagt gctggaggac atttcggcct acaaaaaagt
     3541 gctcatgtag gaaacagccg tgccgacagg tgcacgacca aggtaaggtg ttgataccgt
     3601 atgctgcagg cattcaccca ttgggtgtgg gcgcacatcc gcggcgtcac gctcacgctt
     3661 acagtagtcc tttccaccgc gctgctcata cggggaccac aggcctcgat tggacccctc
     3721 cggtggggaa ttgttcacgt tatcgcggca gggcagagag ttttcggcct ccctattgaa
     3781 ctggcacacc ttcgagatga gaaccgctac ctgcgaagcc tcgttctctc tcacatcatc
     3841 catcaagcgg aaaaccggga actccgaagc gaaaacgaga ggctgcggca attgctgaat
     3901 ttccgtgaac gcagccggtt ctctctcatt gcggcccgcg tacttgcaca cgattcggag
     3961 gttccgccca ccacgataac gattgatgtt ggctttgtgg acggcgtagg tgccaatatg
     4021 ccggtgatga caccggaggg gttactcggc cgcgtcgagg gtcgtccggc cgggcatcgt
     4081 tcgttggtgc ggctgatctc gaacccgagc ctcagggttg cggccgttgt ggagaatgaa
     4141 gatcgcggaa tgggcatact tcggtgcgac gggaagcgat tacgcgtgga aaacgtcgcg
     4201 caggaggcac gggttcgaga gggggacccg gttgttacgt cggggatggg cggcatattc
     4261 cctccgggca tttacatcgg gaaggtcctc aaggtccgca acgatccgca cgcgttgttc
     4321 aaagagatgc tgatttcccc cggagcgcgt ctcgacaggc tggaggaagt ctttgtggtg
     4381 cgggagacct cgccttccgc ggcgataatc ggtggggtgc cggatgcggt ggatcgttga
     4441 ggcattgtgg ctggcggcgg gagccatcgc gtacgccacc ctcgggcagg aaatcgcgct
     4501 gctcggcgcg gtgccgattc tcccgctgat tacgatcgtt cgaatcgcaa tcacctcaga
     4561 cgagcttgcg ggtaacctct ccggtttcgg cgcaggactc cttctggacc ttttctcgct
     4621 ggaatggttt ggcgcatcca tgcttgtcgg aagtgcggtc gggtatacac tcggcgtagc
     4681 acgaaagcac ctcgtcatcg gcaatctggc aacccgtggg attattctcc tcgtgtcggc
     4741 ggaagtattc gcgcttggag tggtgttgat caagagcgcc cggggtgctc ccggtcccga
     4801 accgttcatc actgcgcttg gttccgggat ttacaccgca ctcgtcggca tcgcatggtg
     4861 gttctgcgtg gatcttgcgc gcgggatttt cgggtggcgg gggctgtggg atggtgcgcg
     4921 ctgacctctc cggcatgcga agtgcgagaa tcaaggttct gatggcggtg gttctcatcg
     4981 catttctggc aattaccgtc cagctctaca cactccaggt cctgcgccac gatacactcc
     5041 gcaggcgttc cgaacaaaac gcgatgcgga ccgttcccgt tgaggcaatc cgtggaagga
     5101 ttacggatcg caacggggtg gttctcgtcg ggagccggcc ggcgtacacg gtgggagtga
     5161 tcccggctga agtgaaggac gccgcgcatc ttgacagtat ggtatgcccg atcctgcaga
     5221 ttccaccggg aacagtttcc gggaaggtga gccagcaacg tcagagcctt catcgcccca
     5281 ttgagttgcg ccgggatgct ccgtttcaga taatcgccta cctcgaagag caccggcatg
     5341 agttgcccgg tatcgtgtat ttcgtcgagt cgcgcagaag gtatccgttc gggatacttg
     5401 ccgcccacct gctggggtac gtgcgggaag tccgcgaatc ccagttgaag cagttgaagg
     5461 atgaggggta cgcgctgggc gacctcatgg gccaggcagg tgttgaagcc acctacgaac
     5521 gggagctccg gggcgaaaag ggtgaggagt accaggaagt gactgccacc ggccgcgtcg
     5581 tagacagtca tcggcgtgaa ccggtgcgcg gcatggacgc gaggctcagc attgacctgg
     5641 ctcttcagca ggtcgccgaa tccgcgatgg actcgatacg gcggggcgct ctcgttgcgc
     5701 tcgaccccag aaccggggaa atcctcgcgc tggtgagcag gcctgcattc aacccggaca
     5761 tattctcgac cgtcgttcca cctgagatat ggcgggagct gaacgatgac gtcgagcgtc
     5821 ccctgttcaa ccgtgcaacg atgggaacgt atccaccggg gagcacggcg aagatggtca
     5881 gcgcggtggc gggtctggag accggggttc tggacagctc cacccggttg cggccctgta
     5941 ccgggggact ggctttcgga aatcgtgtgt acgcatgctg gaacaagaga gggcatggct
     6001 cactgaacgt tgtgcacgcg atagaagcct cctgcaatgt ctttttctat caggtgggcg
     6061 aaagggtggg tgtcgacaag tggggaacgt acgcacgatt gctcggactg ggacagaaga
     6121 ccgggataga tctgccggga gaggatgagg gcgttgtgcc gtccacgcag gtatatgatc
     6181 gaaggttcgg gaaagggaaa tgggggcggg gagaggcgct gaacgtgggt atcggccagg
     6241 ggattgtgct tgtgacccca gttcagatgg cacggtacgc cgccgcgctt gcaacgggtt
     6301 atcttgtcac cccacatctt gcgatcggtt ttaccgacac gaacggtgtg gaacatgcgc
     6361 ctgcgttgcg acccccgaaa ccggtttcta tccagcccga tgtgttacgt ttgattcgca
     6421 gcgccatggt cctcgtaacc gagggaggca gcggaaccgc tcaccgggcc gccgtgccgg
     6481 gttttcacgt cgcggggaaa acggggaccg cgcagaaccc ccacggggat gatcactcgt
     6541 ggttcgtcgg tttcgcaccg gttccaaacc ccacgatcgc cattgcggtg atttgtgaga
     6601 atgcggggca tggaagcacg agggcagcac ccgtggccgg ggcggtactt cggaggcacc
     6661 tgctcgggcc cgaacagcct cgccccaaga aggacgagga aatgactgcc gggcatccta
     6721 tcgctcagtt gaggaattga ccacgatggc gacgtcgcga caacagctca ggaagtacct
     6781 cgactggccg cttgtcggcg cggcgcttgg tctggtcgcg tgcgggattg tcgggctctt
     6841 gagcgcgacg acgggtaccg ccaccgcccg cgtcagcggg cttgcgggca tccagtttct
     6901 atggctggtc ctagggatcg gcgtggcgat cggggcgatc cgcgtgccga cgcgcttctg
     6961 gttcgggatt tcctacatcc tgtacgggat cagcattatc gcgctggttc ttacccttgc
     7021 ggtcggtgtt tcgcgcatgg gggcggagcg gtggctcgcc ataggtccct tccgctttca
     7081 gacgtcggag atggggaaga tcgggttgat ccttgcgctg gcccgttacc tctcgtttca
     7141 caaggtgtcg gtttcccgtt ttcgcgactt cgcggtcacc gtcggactgg ttctggtgcc
     7201 gatcgctctt attcatcgcc agccggacct cgggaccgcg cttgtctacg gatccatttt
     7261 tcttcccatg gtctactggg ccggtctggc aaaccttcat ctgctcgcaa taatcacccc
     7321 ggtgataaac gcagtgtgcg cctttgcggg gtggatcccc tggctggtat tcctgctgat
     7381 tctggtcgga gtactcttcc tcatgcggcc acctctccgc tggacagtag tgattgcggt
     7441 gatcaatctg gtcgtgggca ttgtcacgcc ggcaatctgg gaccgcctcg aaccttttca
     7501 gcagcgacgg attgagacat ttttcagccc cgagaaagat ccgctgggcg cgggctatca
     7561 gattatccaa tctcaggtgg cgattggttc aggagggttg tggggcaagg gattcctcga
     7621 aggttcccaa acgcggcttg cgtatctgcc ggagacccac acggatttca tttttgcggt
     7681 gcttggagag gaattcggtt tcgtgggcgc gattgtcatc gtagcgcttt acgttctgct
     7741 gatctggcgc attttccgga tggcacttgt tgccaacaac aacttcgcgt cgtttgtttg
     7801 tattggcgtg ggttcgatgt tcgcgttcca cggaataatc aatatcggta tgacgatcgg
     7861 gcttgcgcct gtaaccggtc ttccgttgct gctggtgagt tacggcggct cgtcgttgct
     7921 gacaaacggg cttgcgatcg gtatggtgct cggtttcggg gtgcggcggt ttgagtaaac
     7981 aggacacgga gaaggatgcc atgaagagtg gggtgcggcg cttgtcgctt ttgttcgtgg
     8041 tgttgcccct tggtgcagtg ctgatgtgga gcgcatgggt tgtggccgcg gcggggtcgt
     8101 cactcaggat tttctacacg gcgagtgtca aagggtacat cgaccagtgc ggctgccggt
     8161 acaaccccac cggcggcatc ggacggagag caacgttcat ccagaggaac acggacggcg
     8221 ccattccgat gattcttctt gacggtggcg atgtcatcgg cgaggagagc cccgttgaac
     8281 gcatgcagac gacgtatctt ttccgggcga tgaaggaaat ggggtaccgg acgatcggtg
     8341 tcggcccccg ggattttgcg tacggcatcg cgtatctgcg tgaggccgaa cggacgtacg
     8401 ggttcacctt cacctgtgcc aaccttgtgg acgacgccac ggggcgcacc ctgttcgcgc
     8461 cccatgtagt ggagcgcgtt tccgggaagg gcgggttccg ccgaaatgcc gcaacggtga
     8521 gggtcggtgt ggtgaatgtg atggggcagg atcgccttcc gctcacggta cccagcgacc
     8581 cgattcttcg cgtcactccg ccggttcccg cggttcagaa cgccgttgcc acgctgcggc
     8641 cacagacgga tgtgatcgtg gtaatggtat acggcacgca gcagttgctc gacagcgtgc
     8701 gcgccgttcc cggggttggc gcggtgctgg caacgcgtcc gctgcgtctt cccgatcagt
     8761 gggtatcgtt caatcgcacg aacgttgtcg ggtactcctc gttccaggga agaggcgtcg
     8821 gaaagattga catctcgctt gccggacccc accggattac ctccgggcga ggcgagatgg
     8881 cgatgatgac cgcggacata cccgaccaac cccgtatggc cgagttgaag cgggagttcg
     8941 aagcgtggaa ggcacgcaac gccgggggag acaggtagcg gtgcgtcgcg ttccagggcg
     9001 ttggtgggct gttttcgggg tcatcgcggc gatcgtggtg ttttttgcga cgctttcgct
     9061 gcctcaggac gcctcccggt tgttcatctt cttcacggcc accgctcacg cgaatcttga
     9121 gccgtgtggg tgccccgtga atccaagcgg gggagtttcc cgtcgcgctg cattcatccg
     9181 gtcgcacact cctcccgaca caccggtgct gctgctggac ggtggggatg tggtcggccc
     9241 tccaacccct gagggattga tcagatcgga gttcatgttc cgcgcgatgg aggaaatggg
     9301 ttattcggtt cttggtcttg gcgcgcggga tttctcattc ggccccgatt ttcttcgttt
     9361 tgccgagaag tactacggct tcacgttcgt cggaacgaat ctgctttctt cccgcacaca
     9421 cgctcacctg ttcgccccgt acgcgattgc gcgggtgggc aatggaagga ttctgggaat
     9481 tccgttcggt ggcctgcggg tcggagtgct cagcgtgatg ggaagggatt taatcccgtt
     9541 tattccggac acgtgcgagc cagcattcct tctggatccg ctggcggctg ttcgaacgtt
     9601 ttcgcggcgc attcgttcgc agtgtgatct cgtcgtggtg ctcgcgtaca cggaccaggc
     9661 tctcctcgac tcactcgtga gcatgcctgg agtagacatc gtcatcgcag cgcggacgct
     9721 taacgtacct cccgggtaca caaatgtcgg tatgagagga agagccgccc ttgcctacac
     9781 cgggtacgaa gctcgtcgaa tcggagtaat gcgtattcgt cttgactcgt cgagaaacat
     9841 gactgaggcg cgcggtgact tgattacgct cagcgagaac cttcctgagg atgaaacggt
     9901 cagagcgatg gtacaggaat cccgccgcgc cgttgcagcc cttcccaacc ccggttccct
     9961 gcagaaagaa ccacccgcgc agtgacgcgg gtccgttatc cagccagaag gagtacctgc
    10021 atgagcacct cgtcccacgg gcgccttgtt gccctttccg tggttttgtt cactgttttc
    10081 ggtgcggcgc tcacgctcac cggcactctt ctcccgcaac tcatcgagca cttcggatta
    10141 tcgtacggag aagccggaac gttgcaggta tcgtcgcaag tcgggtatct ggctgcgatc
    10201 gtcctatcgg ggtggctttc cgcgaaaatt gacgggagaa tgatgttgct tgcagcaatt
    10261 gtgttctccc ttatcggtta cggtgcgttc agccttgcgc cgtcctttcc ggcggcctgc
    10321 gcgttgatga tcgtcggcgg ggcgggcggc ggcgcaatcg aggtcatcat caatgccgtg
    10381 gtgatgaaac tgagcgaaac ccggcggagc aatctactga atttcattca cctgttcttc
    10441 ggagtggggg cattcatagg ccccgccgtg gttggcaagc ttctcgcgct cggcgtgacg
    10501 tggaactggt tctatttcgg gacggcagtg tgctggacag ctctgcttct gggaacactg
    10561 gcatgccgtt tcccttccat aaagccggtg gaaacggcgt cttcagagcc cgcccgcagc
    10621 gtgttctctc ttcctctggc ccgcgtgttg ttcctcgtcc ttacgttgta cgtgggcctg
    10681 gaacttggag ttggagggtg ggtgacgacg tatctggtcc accacgaggg catggctccg
    10741 tcgtacgccg cctattcggc gtcggcattc tggctcggga ttgccggagg acgtctcata
    10801 ctcggcgtaa ttggtcaccg ctggcatgaa tcggggatac tgaccatact ggcggcgttt
    10861 tcggcagcgt gcctgctggc cgcggtgctg gttccgagcc ccttcgtcgc ggcgctgttc
    10921 cttgccctga ccggtctcgg atattccgga atctacccga cggccgtttc cttcggcggt
    10981 ggcgcgtatc cggagcagtc cacaccggtg actgcccttc ttgtcaccgg cgggggcatt
    11041 ggcgcggtgg cattcccgtg gctgatgggg cacatttccg acgggttcgg gttgcggggg
    11101 gggatgctgt tctacatcgt tctgaacatc ctcttactcg ggttcatcct taccgcgcgg
    11161 cggatgcaca gtcgggccca gacgtaatga gcgggagaaa ttgagatgaa aagcttccgg
    11221 aaggagctgt ggttcaacat tccaagccgt cgcgggtttg tgaacatcac aaaggacgtt
    11281 gagcaatgcg tggcggaaag cggggtgacc gaagggctat gcctcgtgaa cgcgatgcat
    11341 atcaccgcga gcgtgttcat aaacgacgat gagccggggc ttcacggcga ctacgaggag
    11401 tggctggaac agattgcgcc ccacgaaccg atttcgcgtt accggcacaa ccgaacgggc
    11461 gaggacaatg gcgacgcaca cctgaaacgg cagatcatgg ggcgtgagac agtcatcgcg
    11521 atttcgcgcg gaaagcttga tttcgggccc tgggagcagg tgttctacgg ggaattcgac
    11581 gggcggcgca agaaacgcgt gctggtgaag ataatcggcg aatagcgaaa ctgagaaagg
    11641 gagagtacat gagcacactg gatttgtttc gtctggacgg gaaggttgcc attgttacgg
    11701 gtgccggacg cgggctcggg agggaaatgg cgattgctct tgcagaagcg ggagcggatg
    11761 tggcgatcac gagcagggaa atgtcacacg tggaaccggt tgctgaagag atcgggaagc
    11821 tttcgggcag acgagtgctt cctgttgtgg ccgacgtgac caggcctgac gacgtgcagc
    11881 ggatggtcga tacgacgtgc agggaactcg gtggtctgga cattctcgtg aacaatgccg
    11941 gggtgaacat ccgccatctc gcccacgagt atacgatgga ggaatggaac accatcatca
    12001 cgacaaacgt gaccggtgtc ttcctttgct gccaggcagt gctcccgaaa atgatggctc
    12061 agaaatcggg acggatcatc aatatcggct cgatgatggg tgtcgtgggg atgccgttgc
    12121 gcgtgccgta ctcggcgtcg aagggggccg tgcaccagat gacgcgcacg ctttcacttg
    12181 aagtggcgaa gtacggtatt acggtcaacg ccatagcccc ggggccattt cttactgaga
    12241 tgaaccggca ggtgattgac cagccggacg tgtacaactt ttttgttgaa cggatacctc
    12301 tcggcagatg ggcggagcca aatgagctga ggggagtggt ggtgttcctt gcctcgcagg
    12361 catcgaacta cattacggga agcgtcatca tggtagacgg tggctggacc gcgcagtagc
    12421 tgaaaactcc gcgcggtatg ccgggtcagt caactggttg gttggaacct gaaggaggcc
    12481 ggacatggaa ctccgtcaac ttgggaaaag cgatctgaag gttacgcccg tcgtgttcgg
    12541 ggcgtgggca atcggggggt ggttctgggg tggcacagac gacgcggagg cggtgacggc
    12601 catccgcgcc gcggttgacg agggcatcaa caccatagat accgcgccga tgtacggttt
    12661 cggccacagc gaagaggtcg tgggaaaagc gctgaaaggg atcagggaca aggttatcgt
    12721 ggcgaccaaa tgcgggctga gatggaaccg cacggatggt gattttcatt ttgtcgcgca
    12781 ggacgagcag aaccgatcgc accagatata caaggtgctg aagcctgatt cgatcatcga
    12841 ggagtgtgat ttgagtttga aacgcctgaa cgtcgacgtt attgaccttt accagtgcca
    12901 ctggccggat acgaccacac ctattgccga cacgatgggt gcgttgatga agctgaaaga
    12961 gcagggcaaa atccgggcag ttggagtgag caacttcact ccagcgatga tgcgcgaatg
    13021 cctcaacgcg gcacctctcg ccagcgacca gccgaagtac agcctgctgg cgcgggaaat
    13081 cgaggcggac gtgctgccct tctgccgtga aaacaatatc ggcgtgatcg tgcacagccc
    13141 gcttgcccaa gggcttctaa cgggcaaagt gacgatggat cggcagttcg ctccgggcga
    13201 tcttcgggtg aacacgccct ggtttcaacc gcaaaaccgc cagcgcgtgc tggacgcgct
    13261 gggaagaatc cgccctattg cggacgcgca ccgagccacg ttggggcagg ttgcgatcaa
    13321 ctgggtgatc aatacgccgg gtgttacgtc ggcaatcgtt ggcgcgcgca acgccgccca
    13381 ggtgcgcgaa aacgttcacg cggcggaatt caggctttcc gaagaggagc agtcgttcat
    13441 acggagcaca ttcgaaagcc tgggaaaacc ggtttgacgg gttcaccgtg aaaaagaacc
    13501 ggggtggcgc ggacataccg tgccaccccg ttcttttcgt ggaccgttca cgtcatgagc
    13561 tcggttgatc ttcactccgt gctctcgtgc cgaacacgac cagatccggg aggtcggctc
    13621 cctttccggc attcgtcagc gcgaactgac catcgttctc acgagaggca accacaaaca
    13681 aatcgggaag gtgtctctcg tctccggccg gttcagcgag gacaatcccg gactggaact
    13741 cggtttccgc ggcaactttt cccgatcgag cgggggagat cccctggtac gggaacgtcg
    13801 cggcaaccag cccggtgagg ccgagtgtag taacaagaag tgatcgtacc atcttcgtgt
    13861 atctccttca atccaagaaa tccagttatt gaagcgaatt tgcagattat ccgccggggc
    13921 tttttacccc tgcacaagaa tagacaacgt gaccgcattg tttgttgcaa ccagagcgct
    13981 cggaggagtc gaactgcacg aggagcagtc tgaactgcag ataccggcat tgcagtgcac
    14041 gccgaaaaaa aagcgcggac gccagggatc acctgacgtc cgccttcacg ggtgtccggc
    14101 ttcttttaga agcttgtcac cacggtatgg tctgccgcga tcgctgaggt gcgggccggt
    14161 cggtccgcga agacggttac atccgggaga tcgccgccct ggatgcggaa ttccagaggt
    14221 gccgccgtgc tcaaggggcc ttcggcaacc acaaccacgt ccggcagatc acccgactga
    14281 ctggccttcg ttacggcgcc cgtagccccg ccggcgattg tcctttcggc cgttaccagg
    14341 aggtccggca gtgttgtttc caccggagca ctgtctgttc tgctttgcgt ggtctttggg
    14401 gccggtaacg ctccaattga aagagccgct gcggccaccg tcaggacgat caggtgaagc
    14461 ttcatcttgt attcctccca tgttgccttt tcctgttccg ttcgcttcgt tcactggatc
    14521 agacgagagg aagacgagat ttgttacact ttccgacgtg cctggccgaa aaagaactta
    14581 gaggagttcg ctgacacgcc cgtaaagttc gggcctgcgg agtcgcagcg tgaaatgggg
    14641 aactgaccgg cggtcgtgaa gcgcttttgc gcgcaggtcc gcaacgagca ttttttctcc
    14701 cggtcccata ccctgtgttc tggcgacgat ttctccttcg gggtcgctga taaaggctcc
    14761 tccgggatat ctgtttgtct caccgtcggg cgtgggaacg gggccggcgt tgttcaccgc
    14821 aacgacgaag cagcccagat cgagcccgcg cgttcgatac gcagcgcatg cggaactcgc
    14881 gagccacaga ttatggttgc tgcccccgta cgcgaacggc atcagaagaa tctccgctcc
    14941 tttgagcgcc agaactgcca ggttctccgg atacaggttg tcattgcaga tggagatgcc
    15001 gactcgcgct ttgccgatgt cgaacaccga aaggctgttg ccttcgtagt agtagggtga
    15061 ttcgtaaggg ttaatgtgcg ttttgcgata ggttccgagg attcctgacg gcgaaaccag
    15121 gacgtgtgtg ttgtagtacc ttccctggca ttcctctgag attcccgcaa ccagcagcac
    15181 gcccatttcg gaggctatct gcaccagtgt atcggtcgca ggaccgggta ccggctgcgc
    15241 gtacctatgc gcgtccctgt gaagccagaa accggtgact gaaagctcgg ggaatacgac
    15301 gagttcagct ccctgttctt tcgcttttgc cgaccattga cggatgtttt ccaggttcgt
    15361 ctggatttcg ccgatgcggc ttaacgaggc tactgctgcg accctgatat cctgcatgta
    15421 atccgtcctt tctcaattcc cattcgccag ggacgcggac gacagcacct cctgcgtcaa
    15481 cgggtcgaga agaccgatcc ggtcctcgtt gctctcatcg gcgcgctgct tttccacaag
    15541 ccggtacagc tcgtccgcca tcgggaaatc aaccgcgacc gtggtgccgg ttgctgagct
    15601 ctggaccgag acgcgtccaa agtgccgacg aacgatttcc gcgacgacgt tgagtcccgc
    15661 ggcttcgagg ctcggctggg tgctgaaaaa aggtccgaag gctttcgaaa tctgattctg
    15721 ggggatgcca gggccggaat cggacacgcg gatgacatac ctgccgtgtt cggctctcag
    15781 acttgtgctc acagtgccgc cgtccggggt tacctccacc gctcttgaga cgagcacgag
    15841 gagtgcctgg taccactggt catggtcgat gtgcggggga gggacgtcgg gcggaatatc
    15901 gctttcgagg atcactcccc gcgcaccggc gtcagcccgg accaccgtgt ataccttctc
    15961 caccagagcg ctgagcagca cgggatgcaa tgttggcagc ggaaccgccg cgagcgacgc
    16021 aacgcctttc accacgttct caaggcgagc gacttccttg atgatgaaat ccagtttctc
    16081 cgcctgggga gagccatcca ctacctcacg cctcaaggcg cgggcgaaac caccgatcgg
    16141 aacaaggggg ccctgcactt cgtggttgat cgccgccacg atgcggccga gagacgcaaa
    16201 atgccgggac tgaagaagga ggtcctgttg ttctttgatc tgctcgctgg ccttttccag
    16261 gttctgcgcg tgccgcgtga cttcctcata caaccgcgca ttcgaaatgg cggatgcggc
    16321 gcttcccgcc atgagtcgta gcagacgcac ggcgtgttcg tcaatcgggc gaccggtgac
    16381 cacattatcc gccagcaccg caccgatggc acgccctccg tgcaggagag gaaccgcagc
    16441 gaacatgggg cttcgcagtt tttcagccac ggagccgccg ggatctcctg aatcaggcca
    16501 gatcagcgac ctcttttctc gcatgcatat gatgaggggg ttctctgctt cttcaagagg
    16561 gatgcgccag tcgcgtgcaa tttcgtgaac aactgagggg gtggtgtcgt cctcagcaag
    16621 cacgaagtcg atgacggatt gaaggtcggg tttgtccgcg acacggggcc acatgcgccc
    16681 ggcttcttcc cagttggaag atcctatgcc gaggcgtgct tcgagcagac tccccgagac
    16741 aaggaaaagt atcgcgcgat tgaaaccgag gccgcttccc gcggtgaagg cggtgagtat
    16801 gatccgaagc acccgctgta tatcgagcgt gctctgcacg gagcgggcaa cgttgtcaag
    16861 catttccatt tcgccgatgc gccagcgaag ttcctgttcg agcgcttttt cccgggcgac
    16921 attccgcgca ttcactgcac tcccgagcag aaccccattt tcgtctctga tgagggtgac
    16981 agtgattttc accggtatct ggtgaccgtc tttcgctttg aacactgtct ggaaattctc
    17041 ggcgaccacg gaatcgtgaa gggattcctc aggctcctcc tcggaaggcg gaccgggtgg
    17101 gatttccggc aacacgatgc tcacttcagc gccgagagcc tcgtccctgg tccaaccgag
    17161 gagcagttcc gccccaaaat tccaggcgcg gatgatgccg gcgtcgtcaa caaagaagaa
    17221 tggatcggca gaccggagga ggacttcggc aagatcgacc ggctggttcg ttcgggaatc
    17281 ggacatctct ctgagttact ctccgccgtg cattcagttc gcgttacggc cctgtcgggg
    17341 cgttgaaggc ccctgatccg agcagcggcg cgacgcccga catgataaac tggactccga
    17401 cgcaaagcag cagaaaaccg agcactttcg agaacgcgag gcggcttgtt tcgctgatat
    17461 gcttttgaag ggccgatgca aaccgaagag cgaggtacgt cacaactgct atcgtgatga
    17521 tggctactcc gaccgcgact tcgttgacga atcgctcaga gcgcgcgctc agtgtcagtg
    17581 ttaccgcgat ggcaccgggg cccgacagga gcggcatcgc catgggcgta aacgcgatgt
    17641 cgtgttttgc gacaccttcg atctgttcgt cggcggagag cctgtctgtt ccgcgaagca
    17701 tctcaagtgc cgcgtaccaa atcatcacac cgccggcgat tcggattgct tccagagtga
    17761 ttgcgaagaa gcgaagaatc caggtcccca ggatttgaaa cgcgatgagt gtcactacca
    17821 tgtaaatgca cgccagaagg gcctgacggt tagtccatgc aggttctctg ttctgggtga
    17881 gtccggcgaa actgaacgcg gcggtaatgg gatccacgat gggaaaaagc gcgaggagag
    17941 cgacgccgta gtgccagagg ccggtgagaa gaaggttcgt gtccattttt ccaactggtt
    18001 tgcagtcggc ccggagagcc gcacacgcat ataagggaag atacacgcac gcaggcgttc
    18061 gtccaaaact gcgtgtgaga aggtggcttt ctggcgaacg gattgctgcg cgtgattaat
    18121 tgcaattgat tctcaatatg aaatgccgcc ccgtcgttgc ccccgcactt cgcgcactaa
    18181 aacccgcaat tcacctgtac ggtgttcccg ctccgctgca tgggccggtt gaaatggaga
    18241 acttgtacgg tcatgtaaga ccgttcgagg aaaggacacg tagatgcttg cactgggact
    18301 tgatatcggc gcgatcagta tcaaactcgc gcttgtcgcc gaccgtgaag acgctgatct
    18361 ggtgcgggga ctggttgaca agaacgacca gtatttcctg ctggaaggtg ttccggaagg
    18421 gaaggtgatc gttctgagcg ggtaccggcg gttgttcgga agtccacggc aatccgcaga
    18481 gaaaatgatc gctgaggtgg ccaccagtat tcccgtagac agggtttcct tcgttggcct
    18541 gacgggaatc ggcgcgtatc cgatcgcgaa gacagccggc cttgcgtcca ttaacgaatt
    18601 ccgcgcggtt gcgcaagcag ttggatatct ccaccccgag gttgagacga ttttcgaaat
    18661 gggcggagag aactcgaaat tcatccggct tgcgcgggac cgtcagaccg gggatgttgg
    18721 aatcgccgat tacgaaaaaa acggtgactg cgccgcgggc accgggtcgt tcattgacca
    18781 gcaggctgcg cgtctcaagt tctccatcga ggaagtgggc ggcgtggtct cgggtgcggg
    18841 caagccgcca acgatcgccg gcagatgctc tgtgttcgcg aaatctgaca tgatccacgc
    18901 ccagcagaag gggtaccaac cgccagaaat cctgaaaggt ctctgtgagg cggtcgcaag
    18961 gaacttcaaa ggggcaatca cgaaggggaa agagatcaag ccgcccatcg cgtttgtcgg
    19021 gggtgttgcc gcaaatgcgg gcgtggttgg cgccatgcgc accgtgtttg agctggaaga
    19081 gcaagatctt ttcgttcctc ccgcatacgc ctggatgacg gccatcggcg ccgccttact
    19141 ggcaccggcc gacgaaaaag ggaaccgggt ggaggagttc ttccgacttc gcgccaaggc
    19201 ttcgggggcg acacacgtcc ttccgacggt gcaaaaactg agcatggaca gggtgatttc
    19261 gctgcgagat cgcgtgaagc cgtacacatt cgaaggcaag cagttgccgg ttccggtaca
    19321 tctgggaatt gacatcggtt ccgtcagcac caacctggtg gtaatcgacg acaacgggga
    19381 cctggtgtac gagatttaca cgtacaccca atcccggcct attcaggtag tggatgcggg
    19441 gctcagggaa atccgcgacg cgatcggaga caagatcagc gtccaatccg caggaaccac
    19501 gggaagcggc agggaactga tcgggctttt gataggcgca gacacgatca acgacgaaat
    19561 caccgcgcac aaaaccggag caagctatgt cggaagtcgc ctgatcaaca ccaaaccgga
    19621 cacgattttc gagattggcg ggcaggattc caagttcatc agtctgaatg aaggtgttgt
    19681 catcgacttc acgatgaatg aggcgtgtgc ggcaggaacc gggagttttc ttgaagaaca
    19741 ggccatgaaa ctcggtgtcc agataaaagg ggagtttgcg cgccgagccc tgtcggcgga
    19801 atctccgaca aagatgggcg agcgctgcac ggtgttcatg gaaaaggacg ttaccgccta
    19861 catgcagcag ggcgccaccg tggatgaaat ctgcgccggg cttgcgtatt ccgtggctct
    19921 caactacttg aaccgcgtcg tgcgcgggag gccgataggg gaacacgtgt ttttccaggg
    19981 gggaacggcg tacaacgacg cagttgcggc ggcgttcacg caaatcctcg acagacctgt
    20041 aattgttccg cctcacaacg gcgttatcgg cgccatcggg atggcccttc tggcgaagga
    20101 cgcggtcgcg cgcaaagggg aacgaaccac gttccgcgga tggagcctgg acgcgatcaa
    20161 ctacgagctt cgggagttca cgtgcaaagg ctgttcgaac cactgtgaca tacagcagtt
    20221 tgacgtggaa ggagaaaaaa cctactgggg agacaagtgc tcggaccgct accggaagag
    20281 cgcgaaggtc aacaagaagc cggtgatcga agacctggta gcgcggcgga tggaggcgtt
    20341 gcttgacggg tatcacgagc cctcggtgag cggccgtgta cgcgtgggta ttcccagggc
    20401 aatgtacttc tacgactggt tcccgttctg gaaggcctat tttgccgctc ttgacgtgga
    20461 tgtggtgctg agcgacacca cctcgaagca ggtcatcaac gagggcacag aagcggccgt
    20521 cgcggagccc tgtttcccca taaaagtggc gcacggacat gtgcataacc tgctggcgaa
    20581 gcaggtggat ttcgtctggg tgcccaacat gatcgacggg gagacggaat tcccggagac
    20641 gaattccatg ctgtgcccgt ggggacagac gatgccgttc gtattccgcg cggcaccgaa
    20701 gttcgagtcc cttacgggca agtggattct gccgacactg cacccgcggc ggggcgaggt
    20761 ggtaatcgag cgggagttgt ggcctgtttc ccagatgttt ggtgcatcgg tggggcagca
    20821 tcgtcgcgcg gtgaatgcgg gttacgaggc cagccgccgc tttcgcgcgc ggttgctcgc
    20881 gctggggcag gaggcgctgg caatcctgcg cgcaaaggac gagatgggaa tcgtgctgct
    20941 cggacgatcg tacaacgtca atgatcccgg gactaatctg aacgtcccga cgaagcttcg
    21001 cacgttgtac ggtacgaatg tcatacccat ggattgtctt ccgatcgtgg gcatcgacat
    21061 caaggatgtg aacgacaaca tgtactggaa ttacggtcgc aaaatcctcc aggcggcacg
    21121 gttcgtttcc cagcagccga atctgcgggt tatctacatc acgaatttca aatgcgggcc
    21181 tgattcgtac atcaagcact acacgaaaga cgcggccggc gggccgtttt tgaccctgca
    21241 gttcgacgga catgcaaacg acgcgggcac gttgacgcgc tgcgaggcgt atctcgacag
    21301 tcaggggttt ttcacgcatg agccgaggcc gatcgagagg ccggcacaga aggctctctc
    21361 gcgaacacga gaagaaaggg ttgaggcatg aggagcaccg gcaacccgtt ggcgggacgc
    21421 acactttaca tacaacagat gacttatgca agcacgcact tgctcgcggc ggcgttccac
    21481 tcagtaggga tcaacgcggt cgtgacggaa gagagcgacg agaggacgct ggaactcggc
    21541 ggcaagtttt cgtcgggcga ggagtgcctc ccggaaaaga tcacgctggg cgattacctg
    21601 aaggtggtct tctcggaagg gtttgacgcc aaacgagccg cgtttctcat gccgacggca
    21661 aatggtccgt gtcgcttcgg gcagtacagt ggtctcctca ggaaggttct gaacgatctg
    21721 gggcatcacg aggcgctgat tttctcaccg acaagcgcga acggttacga tggtttcgga
    21781 gcgaacggca acattttcct ccgatccgcg tggcgggcag tggttgtggg tgacgtactc
    21841 acgaaggcgt tgctcaggac ccgaccgtac gagctgcgaa aaggagacac ggacgaggtc
    21901 tttctgcgtt cgatcaggcg gatgggcgat gttttctcgg agaaagtcga gagtgccgga
    21961 tcgcagttgg agcgcctggt gagggccctt tcggtatgcc acgatgagtt ctgctcgctg
    22021 ccggtggata tgagcgagcc gaagcctctc gtcggagtgg tgggggagat tttctgccgc
    22081 ctgaatgatt tctccaatga ctcaatcgtg cgcaagctcg aggagtacgg atgcgagacg
    22141 tggctgtctg acgttgccga atgggtgtgg tatacaaatg acgaggccaa gacgaacatg
    22201 gatgccgacg gcctcctgaa gatccacccg aaacgaatca aactctgggc gaaaacgaag
    22261 tttcagcgcc acgatgagca cgcgctgctg agcgcgttcg gggactactt tgccggcgcg
    22321 gaggaagcaa gcgacgtgac ggaaatcctg aacgccagcc gaccatatct gccaaaggag
    22381 ggaagcctcg gcgaaatggt gctttcgtcg gggaaagcca tctacctcta ccgtatggga
    22441 gcggatggga ttgtagacat cagccccttc acgtgtatga acggcatcgt gaccgaggcg
    22501 atttacacca agctgagcaa ggattgcgat ggcatcccga tcaggacgtt ttatttcgac
    22561 ggaatatcgt cggaccttga tcg
//
LOCUS       DAOTNG010000010        21584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2506_length_21584_cov_5.363649, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000010 DAOTNG010000000
VERSION     DAOTNG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 21584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21584
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_21584_cov_5.363649"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5179_01105"
     CDS             complement(<1..391)
                     /locus_tag="P5179_01105"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRS93852.1"
                     /translation="MSALLLASVLSYLLGAFPTAIIVGKLLRGIDVRDYGSRNAGATN
                     VWRVLGAGPALFVLAIDALKGAGAVLLIARIASDCCPWDPLTTQVICGMMAVVGHIWT
                     VFAGFRGGKGVGTGAGVFAALAPWCVLI"
     gene            575..1492
                     /locus_tag="P5179_01110"
     CDS             575..1492
                     /locus_tag="P5179_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93853.1"
                     /translation="MSAFTLSLAKFIPFQDAAECARVRAIKKKDLTRHPNPEFRIRII
                     ENIADLNLAFALDIVQRIKKARDEGRKFVAIFPVGPMGQYPIAAEIINSMRLSLDHVV
                     SFNMDEYADAEGNTAPADWEGSFQRAMMESFYGRIHKNLRPSEKNIHFPTKAVIKNYG
                     KMIEDEGEVDVVYGGVGWCGHIAFWEPHLGFEFGNDLDAYMKAGPRLVELHPMTIMQN
                     ALHSFGGDWSWVPPKANTIGPAQIVRAKHRSFWLDGAFGKDSWQRFIARFASHGRVTT
                     LVPATILQTCRTDFTILGAVADNVEVHMA"
     gene            complement(1588..2154)
                     /gene="infC"
                     /locus_tag="P5179_01115"
     CDS             complement(1588..2154)
                     /gene="infC"
                     /locus_tag="P5179_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRS93854.1"
                     /translation="MAPVQKNEDRTRVNQHIRVPEVRLVGADGEQIGIVNTREALRMA
                     EEQGYDLVEVAPNALPPVCRLMDYGKFRYEQQKKTRAITKNRGGEQKEIQMRPNIDLN
                     DYQTKLRHAKEFLAGGDRVKLTVRFRGRELAHRDIGRDLLERFAKDLAGDGRIEGGVF
                     EEGRNTSLNVVPSRSGGSGAAPASASPA"
     gene            complement(2362..4059)
                     /locus_tag="P5179_01120"
     CDS             complement(2362..4059)
                     /locus_tag="P5179_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS93855.1"
                     /translation="MRHSEHLRTPPCHPRLRVLFDLLLFCLVIFVTAPDSAVHAAWAV
                     YGDSWSSATQAQEQVTRVNRVLGEQQASYERATVQGRTYWRVRIGVYATRNEALEARR
                     RAITAGVKEPWMREVSDAELAVQTPSPTPSQTAYADSVRADSLRKVFMDSLARATRRR
                     IDSLGTAAQRQTMDIIEQMRLQITQELVERIEREQARDRSSYVTTAEQSQMTNALIDE
                     MSSRFEVLRDSLRTDRAMRDARDALLPRISGYVAATGIASHDRPAGAGISTSAEVAIP
                     HAQALFEWANDRGGVVLDVRTAGALAVELHQAFVTWSFGATGGASRSLGLGLYEMPYG
                     LEPSFTSDLLTCSPSQVSAAIPPPARGLLLVPHDGERVTLSVFPFGRWDEGTQKGLVQ
                     LALHNEATRFELTAGGERIRYQNEVAPRRYEFAGMANAVIERKGANWLLGAEANLERR
                     RQPVSGILAVNRRQVDTWGGLALVHRRFSERWGWTLRGDFTTQVTTWTNLAVTAPPRE
                     PLARNWTVTSGPIFFLGDRLRARVNYTFDLQEERAVGTYALERSHHHRFEFGLSHVF"
     gene            complement(4099..4605)
                     /locus_tag="P5179_01125"
     CDS             complement(4099..4605)
                     /locus_tag="P5179_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRS93856.1"
                     /translation="MGGGGVVIYDEQLTRINSLIGHLLKESDAKCALLVGKDGRTIAR
                     QGFTRTIDTDQLGALVAGAFSASKAVAELIGEPEFSTLFHQGKHESIQIHLLDPRTLL
                     VVTFDERTTVGMVRLVTKAVSGSLREILQAVYDNPDVEEIGTPEEIASTFDGVFGDSE
                     PKGGSKQP"
     gene            complement(4706..5386)
                     /locus_tag="P5179_01130"
     CDS             complement(4706..5386)
                     /locus_tag="P5179_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HRS93857.1"
                     /translation="MPTIDYANKEISCKIVYYGPGLCGKTTNLQLIHRDVPGINKTNL
                     VSLATEKDRTLFFDFLPVTLGEIKGFKIRFQLYTVPGQVYYNATRKLVLSGVDGIVYV
                     VDSQRSKLQENLDMMQNLRENLAEYRMDLATIPMVLQYNKRDLPGIVPVEELQRAMNP
                     RGLPWFEAVAVQGIGVKETLKAIATLTLSRIGKRTQAAPRDGAAAGPSNATDTGQPKR
                     SIFRKTNP"
     gene            complement(5505..5852)
                     /locus_tag="P5179_01135"
     CDS             complement(5505..5852)
                     /locus_tag="P5179_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRS93858.1"
                     /translation="MKKVLQELGEVEGIVGSLIVDADGIPIVECLDADSELVAATCVQ
                     IVNSVRDALAKLEEDRLVQALIEPAEGKIFLREIPDVGTLVAVSEKNVNVGLIRLALS
                     SASEKIRAYREAL"
     gene            complement(6090..7220)
                     /locus_tag="P5179_01140"
     CDS             complement(6090..7220)
                     /locus_tag="P5179_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS93859.1"
                     /translation="MPEERRLLVVDDEEVITWAISQALTRPGEMRVVTARSGEEALEL
                     MAKEPFDLVVTDIRMKGMTGFELLSRIRETYPESGVIVMTAYGSPEAKREATERGSLF
                     YLDKTFELEEFRSVVRKALEQVDRGRAAVGKGEQEGFSGLIGNLKLIDIVQLHCLARN
                     TVMLEVHAEKVNGRVGFLDGEIVFAETSAGAKGRTAFVDILSWNSGHFENIDEAPAEQ
                     NIDESWESLLIETTALMENSTGEPDVPAPAGADSAEFETPADIHTIVEDLIHTDGIVG
                     AVVAGDTGIVVDSAFDGWDGDDNGIAELASCMAGISRVRFVVEGETQRSHVIIQFDRR
                     RVIAQEVGELGIFLLVISSGTGGLAQHLSATDTAAKKLARLL"
     gene            complement(7365..7715)
                     /locus_tag="P5179_01145"
     CDS             complement(7365..7715)
                     /locus_tag="P5179_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93860.1"
                     /translation="MALPDSPRNRELEAWLRDVTAGIATTILAEVSEDRILAEMESKA
                     GVSDETVVRWGPGVQGQLTGTGKESLNGGNASADGTDRYRFRRPVSEDQGRAVLLFLA
                     SLFGLSDPSEQGDE"
     gene            complement(7802..8890)
                     /locus_tag="P5179_01150"
     CDS             complement(7802..8890)
                     /locus_tag="P5179_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS93861.1"
                     /translation="MGLTGSFRGLSITEMLQLIRMSKKTGALRITLVDNTRHTIYFRA
                     GQVIGVECGRDSLIPFLLERGLLSNDDLNAAISQQKSSSRRAPLEQILLDSGAVTRDD
                     LVQALTSHAEETIGALIQEGGGTISFEDGAQPQLKLEIALDIHNLLMAASVKADEALR
                     DRTLPYVPTDVYAHSGQGKTAFAEKTMRLWVDHWRVFLAIDGNRTLRAISEKIQVAPD
                     ELMKGIAALLTLGYIEPVAGGRARRIALVVDESAMVQKLVQMALAEEDMEIESVSDAD
                     SAHRVLAERTPDVILMNVALPDTSGYRLCRQLRGGDSPCNGVPIILLSARDTPQDEKL
                     AIHAGATAFVSLPFAPESLRNTVRSVLS"
     gene            9205..10458
                     /locus_tag="P5179_01155"
     CDS             9205..10458
                     /locus_tag="P5179_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS93862.1"
                     /translation="MTPRERVRHTLSHREPDRVPIDLGGMGSTNIMAIAYNRLKAFLG
                     IRGGTTRLYDWGQQLCEPESWAIERFHLDVVALSRSLSPVSDPERTREFEARFGGKWK
                     PWVLPDGSLCEAEESFNPEPDGSGGWQMRNERGVWAVMPKSALYFDGGPANHPLDHAK
                     SVADLGDWKPGYAHEDSYKTAAEKAKWLHENTDFAIMAGFGGGALEPAQGLRGWGQFM
                     EDLAGDPDLADEIIGRLTDANVHNVRLFMEMVGPYLDLVQMGDDLGTQAGPQMSPAMY
                     RRFIHPAHKAVYQTAKKTAPNVAIFLHSCGGIRPLIPDLIDEGVEALNPVQTNAAGME
                     PEGLKRDFGGKLTFWGGGAEVAGVLTYGTPDEVRAQVRERIRIFAPGGGFVFCQVHNI
                     QANVPPENIVAMYEAAYEFGKYPIS"
     gene            10504..12333
                     /locus_tag="P5179_01160"
     CDS             10504..12333
                     /locus_tag="P5179_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRS93863.1"
                     /translation="MRKELSLNGCWKFYPAFDEVSGDQKWMDPDFDPTNFEITPKEGM
                     AIGWIKEGFDDSGWLDSPVPSSWNAAFEDLWSYEGHGWYRRKVLIPNEWEGKRVEFFS
                     EGANYRTALYVNGDFAGEHFGGYTPFGFPVHKLLKFGEINTIAIECDNVPKSNRCPGG
                     QFDWWNEGGLYRDVCLRVTDPTYIDDVTVVTRVSSDDVEMTVDIEVCSDDPIFPERSV
                     ALRLSDPAGDSVPVPARISRVVYDAPLKKGHARFCFNVPSARLWSPETPHLYQLDLTL
                     VDTGSEQPRDWWSHRIGLREFRVDGPRLLLNGKPLVVKGVNTYEDYPVAHHSHNEAAL
                     GQDLDLLKWIGGNAFRSHVPAHRRRYELSDERGVLQLVELPFYQWGRPLVQTDNPDAL
                     ADAKGQLRELIKARKNHASVFMWSVSNECLVVPRSNDPQAVSLARMTAEGNIELVKLA
                     HELDPTRPVVEVSNCWPGDPVHEHTDLPCVNVYVGSAPHVNGLPVVAERIREKFEALR
                     KDHPDKPILAGEWGSWCVRGVKTDYFPGEVFQAEKIRLFWETMREEENFIGGFIWCFA
                     DYDVHRRFLWVYEYRCAYGLFDYHRRPKEAAYAVRRMWTGKGN"
     gene            12333..14174
                     /locus_tag="P5179_01165"
     CDS             12333..14174
                     /locus_tag="P5179_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRS93864.1"
                     /translation="MRPHISLNGLWKFCPAFDAISADQRWLDSDFDPKNPDLTPVTAG
                     AARGWIKPDYDDSRWLDITVPNSWNAAIRDLWSYEGHGWYRRTIHIPRAWKDKRVEFV
                     SEGANYRVALYVNGELAGEHEGGYNPFSIPIHRFLKFGARNLIAVICDNIPKPYRSPG
                     GQFGWWNHGGLYRDVSLRVTDTTYIGDVTVVTDVNGSHVDVGVTVEVACEAGEMTTRR
                     IEIELDDPSGKPVPLPSERNAQTVTLAGGIAKAAFTFALDGVLLWSPAEPNLYALRVK
                     LLEDGCTTDEWSHRIGLRTFRIDGTRLLLNGEPFLVKGVNRHEQYAGGTIHTPTHTEA
                     QISEDVDLIKWLGGNAIRQHYPNHRRLYELCDERGIVCMVENPLWQWGRPLVETDNAD
                     ALATAKRQLTEIIRALKNHASVFMWSVSNENLVKAKSDDAKVVALANQTIEGNKELVA
                     LAKKLDPTRPVVEASNEWPGDAVLCETDISAVNVYVGNPVPHVSGAPKIVELMKAKMQ
                     KLREEIPGKPILAAEFGEWTVRGLEQDYPPGENYQTAKLTALWKGFMEQPDIIGGFIW
                     VFADYDLHRRFLWAYEYRCAYGLFDIERKPKAAAYAIRELWTAGNGR"
     gene            complement(14278..15258)
                     /locus_tag="P5179_01170"
     CDS             complement(14278..15258)
                     /locus_tag="P5179_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRS93865.1"
                     /translation="MRIAVVGTIVYDEIVTHRGDRKQSFGGITYNIAALESIIDEHTQ
                     LVPIAQVGYDRYEDVVRLYSSYRGVVMDGLRRMPDQKNAHVQLIYKSVTERTESMQNV
                     PPPLAPEQLELAASCDAVLVNFITGRELEAESFQRLGTRAKYLHLDIHNKIADWTPEG
                     RRVFVGLPEWREYVSRATTVQMNEFEIEQLLGRPVKGTKEYLAAAAEVLDAGPQGVMI
                     TLGPLGSVVAYRSGDAVYGTAWPAANLGEVLDTTGCGDSFSAGFVWSYLRRNDVLWAN
                     AAANIVGDVNCITPGIGNLTKAREMDRLVPEAFGELAKKLAAGWKGERLV"
     gene            complement(15351..16172)
                     /locus_tag="P5179_01175"
     CDS             complement(15351..16172)
                     /locus_tag="P5179_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRS93866.1"
                     /translation="MYVGLLTAPFGKEPIDKVIEFAAENGFAGLEITAGPGAHIDTTR
                     FNANEAERVKELVAKSNLKISSLAYYVNICDGRAETSEHYLKVIDAAAMLGTDVVCGM
                     AGMPVQGKSKEDTIKEVVPKVYRPILDHAAEKGIKIAMENWYATNIQHLGLWDLIFDV
                     VPDKHFGLNFDPSHLMWQGIDYLGAVERYRDRIFHTHAKDTEIKQHVLRHVGYLAGGW
                     WRYVIPGFGEIDWGVYIERLYAIGYKGVLSIEHEDGAQGREEGFIRGLRHLKQWV"
     gene            16352..17755
                     /locus_tag="P5179_01180"
     CDS             16352..17755
                     /locus_tag="P5179_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93867.1"
                     /translation="MPAILTSEPHLFVDLNEVEERCNITRNFHTARKYGRHPVLKQDA
                     PWEKHPGMTASVIYDVEEKVFKAWYMAGFYEPGSKHVQCYAVSDDGIHWNKPALGLHE
                     ALGSKENNIVIPSSYHDGMDHWESMLKDPLDSNPARRYKALGWSSYDWDGPLSGIYTA
                     TSPDGLNWEHTPEPVFRFHPRPGRNDLGPVGDAQGLMIDTLKKRYVAFLRTGISRSLS
                     ISEDFVRWTAPRPFLQSLNEEETLYNNVGFVYGNQYLGFLTHFDRNAYHQTQTLQLLT
                     SRDGETWMRPFADPVVPTSDVGEWDRFQILLTGAPPIAVGDQLFIYYRGTARRHNKVK
                     GEFEPRIDSDQDRRTMSIGLATLRLDGFASIAASYDGGYITTRPTVLGGDVLSVNAVS
                     DYGEVRLEILDEKGETIPGYSAQDCIPVSVDCVEAEVAFRDRRSLSEISGRTIKLRFH
                     LKNARLYSFRATYSSHG"
     gene            18061..18501
                     /locus_tag="P5179_01185"
     CDS             18061..18501
                     /locus_tag="P5179_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRS93868.1"
                     /translation="MTLVRYFPGFARPVFGRPTDELARWPSDWPFDAPLNAEWYPRAD
                     VVETDNAWEISVELPGIEKDAVKVSVENGVLSISGETKAEHKDEGRGVYRLERRYGSF
                     SRTFELPDGVDAEKIEAVFKNGLLKLTVPKTAEVLPKTIEIKHE"
     gene            complement(18735..20081)
                     /locus_tag="P5179_01190"
     CDS             complement(18735..20081)
                     /locus_tag="P5179_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRS93869.1"
                     /translation="MSGETPSNPREDATRIVLAAISAADPKEGVKRSFRIAANELAVG
                     TWHRRTEEIGRIRVVGAGKASAHMAAALEEVLGDRVTSGIVITKYGHGVPLRRIKVVE
                     AGHPVPDEQGVEATLELMQHVAGSEPDDVVFVLLSGGASSLLVAPAQGITLADKKTTT
                     SVLLSCGATIEELNCVRKHLSRVKGGQLLRACGSAQVVSLILSDVIGDPLDVIASGPT
                     VPDPTTFGDALGVIDRLGIRGSLPPRVVSHLEAGDRGEYPETPKPDEGSFSNGHALLV
                     GTNSIALKSAAYEAERRGYRAHVLTSSLRGEASEAAKVVCALAEGVPAGERPTALILG
                     GETTVTLSRDAGKGGRNQEFALSAAREIRGRSDITVASVGTDGTDGPTDAAGGLVDGA
                     TWERALRRGLSPDAALRRHDAYPLLSATGDLVMTGPTGTNVMDVIVALAVPRKNTF"
     gene            complement(20078..21517)
                     /gene="purB"
                     /locus_tag="P5179_01195"
     CDS             complement(20078..21517)
                     /gene="purB"
                     /locus_tag="P5179_01195"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRS93870.1"
                     /translation="MPNETAPRYADPLVSRYASPEMASIFADLTRFRQWRRLWIALAE
                     AEKELGLPISEEQLAEMRAHAEDIDIARADELERKLRHDVMAHVHAFGEQCPGARPII
                     HLGATSCYVTDNSELWQIREGLRLLRARIVRLITNLADFARTWRSQPALGYTHFQPAQ
                     LTTVGKRATLWLHDFVEDLLEIEYRLDRLAFRGLKGTTGTQASFLELFDGDHARVREL
                     EARVARKMGFERVAPVTGQTYSRKQDWQVLQALSGVAQSVHKFGNDIRLLQHLKEIEE
                     PFGSSQIGSSAMAYKRNPMRSERATGLARWVLNVVQNASMTASEQWFERTLDDSANRR
                     LAIPHAFLGADALLILTTNIVKGLVVYPAMIEQHIRQELPFMATENLLMAAVRAGGDR
                     QLLHETIRVHSQAAGDAVKAGGRNDLLERLANDPGFPLESAEIARELEPKRFVGRAPE
                     QVDEYLDEVVAPILAARANLKPPETEVKV"
BASE COUNT         4466 a         6377 c         6166 g         4575 t
ORIGIN      
        1 cgatcaggac gcaccacgga gccaacgccg cgaagactcc cgcaccggtt ccaacgcctt
       61 ttcccccgcg aaaccccgcg aacaccgtcc agatgtggcc aaccaccgcc atcatgccgc
      121 agatgacctg tgttgtcagg ggatcccacg ggcaacaatc gctggcgatc ctggctatca
      181 gaagcacggc ccccgcacct ttcagggcgt caattgccag aacgaaaagt gcaggccccg
      241 caccgagcac gcgccagaca ttcgttgccc ccgcattacg actgccgtag tcgcggacat
      301 caatcccgcg caacaacttg ccgacgatga tcgcggtggg aaatgcaccc aggaggtaac
      361 tgagaacgct tgccagcaag agcgcagaca tgcttgatcc taccccgcga aaactatgcc
      421 ttcgttcatg accgtagagt atacataact tacggacggc ccgccggagg agcaaaacaa
      481 cccggtgcct ctttttcctg cctggcgcgt tggctatcct ttcgaatgcg gttccggcat
      541 acggtgcagc cttcaaaaac gaaaggagtg cgtcatgtca gccttcacgt tgagcctcgc
      601 caagttcatt ccttttcagg acgcggcgga atgtgcgcgc gttcgggcca tcaaaaaaaa
      661 ggacctgacc agacacccga acccggaatt ccgcatccga atcatcgaaa acatcgcgga
      721 tctcaatctc gcgttcgctc ttgatatcgt gcagcgaatc aagaaggccc gcgacgaggg
      781 aagaaagttt gtcgcgattt ttcctgtggg tcctatgggc cagtatccca ttgcggcaga
      841 aatcattaac tcgatgcggc tatcactgga tcatgtcgtt tctttcaaca tggacgaata
      901 cgccgacgcc gaggggaaca ccgcgcccgc ggactgggaa ggatctttcc agcgtgcgat
      961 gatggaaagt ttctacggcc gaatccacaa gaaccttcgc ccgagcgaga agaacattca
     1021 ctttcccacg aaagcggtta tcaagaacta cgggaagatg attgaagacg aaggggaagt
     1081 ggacgtggtg tacggcggtg taggctggtg tggtcacatc gccttctggg agccacatct
     1141 cggttttgag tttggtaacg acctcgatgc gtacatgaag gccggtccaa ggttggtgga
     1201 actccatcca atgactatca tgcagaacgc gctccacagc ttcgggggcg attggtcctg
     1261 ggttcctccg aaggcgaaca ccatcggtcc cgctcagatt gtacgagcca agcaccgaag
     1321 tttctggctg gatggagcat tcgggaagga tagctggcaa agattcatcg cccggtttgc
     1381 atcacacggg cgcgtcacca cgctggtccc cgcaacgata cttcagacgt gccggacgga
     1441 cttcacaata ctgggtgctg ttgccgacaa cgtcgaagtt cacatggcgt gaatcctgcg
     1501 ctgacgaagc gaaagaaaac gagccatccg tacgataccc ggatggctcg gttgtttctc
     1561 gtttatcgag tcggcgtcaa gaactcttca ggcgggagaa gctgaagcgg gagccgctcc
     1621 gctgccaccg gatcgagaag gaaccacgtt aagactcgta ttacgacctt cctcgaaaac
     1681 gcccccttca atccggccgt ctcccgcgag gtctttcgcg aaccgctcaa gaagatcccg
     1741 cccgatgtcg cggtgagcga gttctcgacc gcggaatcgc acggtcagct tgaccctgtc
     1801 gccacctgcc agaaactcct ttgcgtgccg caacttcgtc tgataatcgt tcagatcgat
     1861 gttcggcctc atctggattt ccttctgttc cccgccccgg ttcttcgtaa tcgcccgggt
     1921 tttcttctgt tgctcgtatc ggaatttccc gtaatccatg aggcggcaca ctggaggtaa
     1981 cgcgttcggg gcgacctcca cgagatcata cccctgctcc tccgccattc ggagcgcctc
     2041 acgagtgttg acaattccga tctgttcgcc gtcagctccg acaagccgta cctcggggac
     2101 cctgatatgc tggttcaccc gtgtgcggtc ctcatttttc tgtaccggag ccatcggttt
     2161 gggcttccgc attagaacga tgtgagcacc tcgtctgtga tgggttgaga cgggcaaccg
     2221 gccaaatgtt cgcaccggtg ccagaacatg cgaaaaagat agcagcatac aggggcacgg
     2281 tcaacatagc ggcccgcggg aaggatgaac gacgcgtgcg attggcgaag tctgttgtgc
     2341 acctgcggtt cattgtgcgg gtcagaacac gtggctcagc ccgaactcga agcggtggtg
     2401 atggcttcgt tcaagggcgt acgttcccac tgcccgctct tcctgaagat caaacgtgta
     2461 gttgacccgc gcgcgaagcc tgtcccccag gaaaaagatg ggaccgctcg tgaccgtcca
     2521 gttccgagcc aatggttctc ttggaggtgc cgtcacggcc agattcgtcc atgtcgtaac
     2581 ctgggtggtg aagtcccccc gcagcgtcca tccccatctt tcgctgaaac gccggtgcac
     2641 cagtgcaagt ccgccccatg tgtccacctg cctgcggttc actgccagga ttccggatac
     2701 cggttgacgt cgccgctcca gattcgcttc cgcgcccaac agccagtttg cgcccttgcg
     2761 ttcgatcacc gcattggcca tgccggcgaa ctcgtaccgc ctcggagcaa cttcgttctg
     2821 gtaacggatc cgttcgccgc cggcggtgag ttcgaacctc gtggcctcgt tgtgcagcgc
     2881 cagttggacc aaccccttct gtgtcccttc gtcccaccgg ccgaacggga aaaccgacag
     2941 tgtaactcgc tccccgtcgt gcggaacgag gagaagcccg cgggcaggcg gagggatggc
     3001 ggcgcttacc tggctgggcg agcacgtcag caggtctgac gtgaacgacg gctcgagccc
     3061 gtaaggcatt tcatagagac caagccccag agagcgactc gcgcccccgg tggccccgaa
     3121 ggaccaggtg acgaacgcct gatgcaactc cacggcgaga gcgcccgccg tgcgcacatc
     3181 aagcaccacc ccgcctctgt cgttcgccca ctcgaacaga gcctgagcgt gcggaatggc
     3241 gacctctgcg ctcgtactga tgcccgcccc ggccgggcga tcgtgtgaag cgatccccgt
     3301 ggcggcaacg taaccgctta tacggggcaa aagcgcgtct cgggcatctc tcatcgctcg
     3361 gtctgtccga aggctgtccc tgagcacctc gaaacggctg ctcatttcgt caataagggc
     3421 attggtcatc tgcgattgtt cggccgtggt aacgtacgag gagcggtcgc gtgcctgctc
     3481 gcgctcaatt cgttccacca gttcctgagt gatctggagc cgcatctgct cgatgatgtc
     3541 cattgtctgg cgctgcgcag cggtgccgag gctgtcgatc cttcgccttg tggctctcgc
     3601 cagcgaatcc atgaacacct ttcgaagcga gtccgcgcgg actgagtcgg cgtacgcggt
     3661 ttgcgaaggg gtgggagatg gcgtctgaac cgcaagttcc gcgtcggata cttcccgcat
     3721 ccagggttcc ttaactccgg cggttattgc tctccttctg gcttcgagcg cctcgtttcg
     3781 cgtcgcatag acgccgattc ggacgcgcca gtacgttcgc ccctggaccg tcgcgcgttc
     3841 atagctggcc tgctgctccc cgagaacccg gttgacgcgc gtaacctgtt cctgggcctg
     3901 cgtggcggag ctccaactgt ctccgtacac cgcccacgcg gcgtggaccg cggaatcagg
     3961 cgccgtcacg aagatcacaa gacagaagag caggagatcg aaaaggacac gcagtctggg
     4021 atggcagggc ggcgttctga ggtgctccga atgtctcatg gtaatttccc ggggcaaaac
     4081 ccttcggtca ggcacgtact agggctgttt cgacccaccc tttggttccg aatctccgaa
     4141 aactccatcg aacgtgcttg cgatttcctc gggcgtaccg atctcttcca catcaggatt
     4201 gtcgtacacg gcctgcagta tctcccgcag actgccgctc acggctttgg tcaccagcct
     4261 taccatccct acagtcgttc tttcatcgaa ggtgacgacc agaagtgtcc gggggtccaa
     4321 caggtgaatc tggatgcttt cgtgtttccc ctggtggaaa agagtggaga actcgggttc
     4381 gccgatcaat tccgccactg ctttcgaagc cgaaaatgca ccggccacga gtgccccaag
     4441 ctggtctgtg tcgatggttc ttgtgaagcc ctgtctggcg atcgttctgc cgtccttgcc
     4501 cacaagaagt gcgcatttcg cgtcggactc tttgagcaga tgcccgatca acgagttgat
     4561 ccgggtaagc tgctcgtcat aaatgactac cccacctcca cccatggagt gtctcctccc
     4621 taatgcctca gcgtgctcac aggccaggaa atccttgcgt ccaaacgcat tgatgccaaa
     4681 cgaagaacgg tgctggagtg gattttcatg ggttcgtttt ccggaatatc gagcgttttg
     4741 gttgacccgt gtcggtagcg ttggagggac ccgccgcagc gccatctcga ggcgcagcct
     4801 gggttctttt gccgatccgt gataacgtaa gcgttgcgat agccttcagc gtctctttga
     4861 ctccgatacc ctgcacggcc actgcctcaa accacggaag accacgaggg ttcatcgctc
     4921 gctgaagctc ttccacggga acgatgccgg gcaggtcacg tttgttgtat tgcagaacca
     4981 tgggtatcgt ggcgagatcc atgcggtatt ccgcaagatt ctcgcggaga ttctgcatca
     5041 tatccaggtt ttcctgcagc ttgctccttt gcgaatccac cacatacaca atcccgtcca
     5101 cgccgctcag gacgagtttc cgtgtggcat tatagtacac ctgacccggt accgtgtaca
     5161 actggaacct gatcttgaag cctttgattt cgccaagggt tacaggcagg aaatcgaaaa
     5221 agagggtccg gtctttctca gttgccagag agaccaggtt cgttttgttt attcccggga
     5281 cgtcgcggtg gatcaactgg agattggttg tcttgccgca caagccggga ccgtagtaca
     5341 cgattttgca actaatctct ttgtttgcgt aatctatggt cggcattcat gacccccggc
     5401 attccgattc atcatcttca caacgcttcg ggccggctcc tgaaggagcg gatggttccc
     5461 accggaggtg gccacgaaac agcctttttt cgcacatctg cggatcagag tgcctccctg
     5521 tatgcgcgaa tcttttcgct cgcgcttgaa agagccagcc ggatcaaccc cacgttgacg
     5581 ttcttttcag acaccgccac gagtgttccg acgtccggaa tctcgcggag gaatattttt
     5641 ccctcagcag gttctatgag cgcctgcacg agtctgtctt cttcgagttt ggccagggcg
     5701 tcacgaaccg aattgactat ctgaacgcat gttgccgcca cgagttcgct gtccgcgtcg
     5761 aggcattcca cgatcgggat cccgtcggcg tccacgatga gactccccac aataccttca
     5821 acttcaccaa gctcctgcaa aacctttttc atgggaactc ctccggaacg ccagattcaa
     5881 tgcgataacc cggttgccat gttgcccctc acaccgctga gatggggcgt gattcgacgt
     5941 cgttcttacg aaaagagatg tacacaaacg aggggggtaa gtcaacggag cccgttcttg
     6001 cggcacgttg attgctggtt ctcgcccgca ccgcacgtca cgctcgaacc caaactcgca
     6061 ctcgttaagt taaatactga aaatggcttt caaagaagtc tcgccagctt ttttgctgcc
     6121 gtgtccgtgg cagaaagatg ctgcgcgagt ccaccggtac ccgaactgat taccagcagg
     6181 aaaatgccga gttcgccaac ttcctgtgca atgactctcc tacggtcgaa ctggattatg
     6241 acgtggctcc gctgtgtctc accctcaacg acgaaccgta cgcgggagat tcccgccatg
     6301 caactggcca gttcggcaat tccgttgtca tcgccatccc aaccgtcgaa cgcgctgtca
     6361 accacaattc cggtgtcgcc ggcaactacg gcgccgacga tgccatccgt gtggatcagg
     6421 tcttcgacga tggtgtgaat atccgcaggc gtttcgaatt ctgccgaatc ggctcctgcg
     6481 ggagcaggga catcgggctc accggtgctg ttttccatga gtgcggtggt ctcgataagc
     6541 aaggactccc agctttcatc gatgttctgc tctgcgggcg cttcgtcgat gttctcgaaa
     6601 tgccccgaat tccagctcag tatgtcgaca aaagctgtcc ggcctttggc ccccgcgctt
     6661 gtctccgcga acacaatttc cccgtcgagg aatcctaccc gaccgttgac tttttccgca
     6721 tgcacctcca gcatcaccgt atttcgcgca aggcaatgca actgcactat gtcgatcagc
     6781 ttcagattgc cgatgaggcc gctgaaaccc tcctgttccc ccttaccaac ggcagcccgc
     6841 ccgcggtcca cctgttccag agccttccgc acgacactcc ggaattcctc cagttcgaac
     6901 gtcttgtcga ggtaaaaaag agatccccgt tccgtcgcct ctcgtttcgc ctccggtgag
     6961 ccgtaagccg tcatcacgat cacaccggat tcgggatagg tctcgcggat tctcgaaagc
     7021 agttcgaaac ctgtcatgcc tttcatgcgg atgtctgtga cgaccagatc aaacggctct
     7081 ttcgccatca attccagcgc ttcttctcca ctgcgggcgg tcaccacccg catctctccc
     7141 gggcgagtga gcgcctggga tatggcccat gtaatcactt cttcatcatc gacaacaagc
     7201 aggcgccgtt cttctggcat ttctcggttc ctcgggcagc caaacactcg gatactggga
     7261 aaaatcggcg ttcacattcg aaaaaactga agaccgtaag ttattgcgcc acaatacaaa
     7321 aggggcagag acgtaaccct ccccaaacgg tttccggaca cggtttactc gtcgccctgt
     7381 tctgatggat cggacaaccc gaacagcgac gcgagaaaaa ggaggacagc ccgcccctga
     7441 tcttccgaaa cggggcgccg gaaccgatac cgatcggtac catccgccga cgcgttgccg
     7501 ccgttgaggg actccttacc ggtaccggtc aattgcccct gcacgcccgg tccccagcga
     7561 acgacggtct catcgctcac tccggccttt gattccattt ccgccagaat tcggtcttca
     7621 gacacttccg ccaggatcgt agttgcgatg cccgcggtca cgtcccttag ccacgcttcc
     7681 agttcccggt tgcgtggact gtccgggaga gccatggtga cctccttctg tcaacgcgca
     7741 gcgatcgact ccgtagcaac aaccaacccg gtggcgcgaa aaacaacctc cgtccggaac
     7801 ttcacgaaag cacactcctt accgtgttgc gaagtgattc cggtgcgaac ggcagggaga
     7861 cgaaggctgt tgcccccgcg tggatcgcga gtttctcatc ctgtggcgta tctcgagcgc
     7921 tgagcagaat gatcggcaca ccgttgcagg gtgaatcccc gccgcgcagc tgtcggcaca
     7981 accggtagcc gctcgtatct ggcaacgcaa cgttcatgag gatgacatcg ggcgttcttt
     8041 ccgcgaggac gcggtgtgcg gagtctgcat cactcacgct ctcgatttcc atgtcctctt
     8101 ccgcgagggc catctggaca agcttctgca ccatggcgct ttcgtcaacc accagtgcga
     8161 tccggcgagc gcgccctccg gcaaccggtt cgatataccc cagagtcagc agcgcggcaa
     8221 tacctttcat caactcatcc ggcgccacct gaatcttttc cgatatggca cgcagggtac
     8281 gattcccgtc gatcgccaga aacacgcgcc aatggtctac ccataaccgc atcgttttct
     8341 cagcgaacgc ggtttttccc tgacctgagt gcgcgtagac gtcagtcggc acatagggaa
     8401 gcgtccgatc ccgcagcgcc tcgtccgcct tgaccgaggc cgccatcaac aggttgtgaa
     8461 tgtcgagcgc aatttccagt ttgagttgcg gttgtgcgcc gtcctcgaac gaaatggtgc
     8521 cgccaccttc ctgaatcagc gccccaatgg tttcttccgc atgcgaggtg agcgcctgaa
     8581 ccaggtcgtc cctagtcact gcccccgagt ccagaagaat ctgctcgaga ggcgctcttc
     8641 gagaagaaga tttctgctgc gatattgcgg cgttcagatc gtcattcgag aggagacccc
     8701 gttcgagcaa gaatggtatc agactgtccc ttccacattc gacgcctatc acctgtccgg
     8761 cgcggaagta gattgtgtgt cgcgtgttgt cgacgagcgt gattcgcaat gcacccgttt
     8821 tttttgacat ccggatgagc tgaagcatct ccgtgatgct gaggcctcgg aaggatcccg
     8881 tcaaacccac agttcttcac tcctcgtata ccagaaacgc gagccttgcg ctcgcgggca
     8941 ccggcaggcg gcgagaagga caatcgcggc gcggtttccg aaaaagcatc aaccgcccga
     9001 cttgcgcccg ggggcgaatc ctttgttccc gtcggtgttt tctgaagata cgacacggcc
     9061 gctcaagagt gaagacgtcg cacccgcctg aacacggggt ttttcgcaga tatccctgct
     9121 ggagccttcc cgtccgttcg agaccggtcg taatttcact agacctgcgt gcattggcct
     9181 ttcacaacag ggagacacca agctatgacg cctcgcgaac gcgtccggca cacactttct
     9241 caccgcgaac ccgacagagt ccccatcgat ctgggcggaa tgggttcgac caacatcatg
     9301 gcgatcgcgt acaaccgtct gaaggctttt ctggggattc ggggaggaac cacgcgcctt
     9361 tacgactggg gacagcagct ctgtgaaccc gaaagctggg ccatcgagcg atttcatctg
     9421 gacgtggtgg cgctgtctcg gagtctttcc ccggtatccg atcccgagag aacgcgcgag
     9481 tttgaagcgc gcttcggcgg aaaatggaag ccctgggttc ttcctgacgg ttcgctgtgc
     9541 gaagcggagg aatcgttcaa cccggaaccc gacggcagtg gcggctggca gatgaggaac
     9601 gaacgtggcg tgtgggctgt catgccgaaa agtgcgctgt atttcgacgg cgggccggcg
     9661 aatcatcctc tcgatcacgc gaagagcgtg gcggatcttg gagactggaa gcctgggtat
     9721 gcacatgagg actcgtacaa aactgcggcg gaaaaggcaa aatggctcca cgagaacacg
     9781 gactttgcga tcatggccgg ttttggtgga ggagctctgg aacctgcaca ggggttgcgg
     9841 gggtggggac agttcatgga ggacctcgcc ggcgatcccg atctcgcgga tgagatcatc
     9901 ggacgtctga ccgacgccaa cgttcacaac gtgagacttt ttatggagat ggtggggccg
     9961 tatctggatc tcgttcagat gggggacgat cttgggaccc aggcgggccc gcagatgtcg
    10021 ccagcgatgt accgccgctt catccatccg gcgcacaaag cagtttacca gacggcgaag
    10081 aagacagccc cgaacgtcgc aattttcctc cattcctgcg gtgggatacg cccgttgata
    10141 cctgatctga ttgatgaagg tgttgaggcg ctgaatccgg tacagacgaa tgcggcggga
    10201 atggaaccgg aaggattgaa gcgcgatttc ggcggaaagc tcacattctg gggtgggggt
    10261 gctgaagtag caggggtgct gacgtatgga accccggacg aagttcgagc gcaggtgcgg
    10321 gagcgcatcc ggattttcgc gccgggagga ggatttgtct tctgccaggt gcacaacatt
    10381 caagcaaacg ttccacccga aaacatcgtc gcgatgtatg aagcggcgta cgagttcggc
    10441 aaatacccca tttcgtgaga cgatgtaccc ctggcactgc gcacaaaaag ggagtgacac
    10501 accttgagga aagagctcag tcttaacggc tgctggaagt tctaccctgc gttcgatgaa
    10561 gtgagcgggg atcagaagtg gatggatcca gatttcgatc ccacgaactt cgaaatcacg
    10621 ccgaaggaag gaatggccat cggctggatc aaagagggat ttgacgattc ggggtggctg
    10681 gattcacctg ttcccagcag ctggaacgcc gcattcgagg acctgtggag ctatgaagga
    10741 cacggttggt accgccgaaa agttctcata ccgaacgagt gggaaggcaa acgcgtggag
    10801 ttcttttcgg aaggggccaa ctatcgcacc gcactctacg tcaacggcga tttcgcggga
    10861 gagcacttcg gcgggtacac tccgttcgga ttcccggttc ataaactcct caaattcggc
    10921 gagatcaaca ccatagcgat tgaatgcgac aacgttccaa agtcgaaccg ctgccctggc
    10981 ggtcagtttg actggtggaa cgaagggggg ctctatcggg acgtgtgcct gcgtgtcact
    11041 gatcccacct acatcgacga cgttacggtg gtaaccagag tctcgtcaga tgatgtggag
    11101 atgacggtcg acattgaggt gtgttccgac gacccgattt tccctgagcg cagcgttgcc
    11161 ctgcgcctga gcgacccggc gggtgattcg gtccccgttc cagcgcgcat ttcccgcgtc
    11221 gtgtatgacg cgcccctgaa gaaaggccac gcgcggttct gcttcaacgt cccttcggcg
    11281 cgtttgtgga gtccggaaac tccccatctg taccaactgg acctcacact cgtggatact
    11341 ggatctgaac aaccccggga ctggtggtct caccggatag ggctccgcga gttcagagtg
    11401 gatggtcccc ggttgctgct taacggcaag cccttggttg tgaaaggcgt aaacacctac
    11461 gaggattacc cggtggcgca ccactctcac aacgaggctg cactgggaca agacctggat
    11521 ctcttgaaat ggattggcgg gaacgcgttc cgttcacacg ttcccgcgca ccggcgtcgg
    11581 tacgaacttt ctgacgaacg aggtgttctt cagctcgtcg aactgccgtt ctatcagtgg
    11641 ggaaggcccc ttgttcaaac ggacaacccc gatgcgctcg cggatgcgaa agggcaattg
    11701 cgcgagttga taaaggcgag gaagaaccac gcgagtgtgt tcatgtggag cgtgagcaat
    11761 gaatgcctcg tcgtcccgcg ctcgaatgac ccacaggccg tttcactcgc cagaatgact
    11821 gcggaaggga acatcgaact cgtcaagctt gcgcacgagt tggatccgac gcgcccggtc
    11881 gtcgaggtga gcaactgctg gccgggtgat ccggttcatg agcataccga ccttccgtgt
    11941 gtcaacgtgt acgtcggttc agctcctcat gtaaacggcc ttcccgttgt ggcagaacgg
    12001 attagggaga aatttgaagc tctgcggaag gatcatccgg acaagcccat ccttgccggt
    12061 gaatggggaa gctggtgtgt ccggggagtc aaaacggatt atttccccgg agaggtcttc
    12121 caagctgaga aaatccggct gttctgggaa acgatgaggg aagaggaaaa cttcatcgga
    12181 gggttcatct ggtgcttcgc tgattacgat gttcaccgac ggtttctctg ggtatacgaa
    12241 tatcgctgcg cgtacggatt gttcgattac caccgcaggc ccaaagaagc cgcgtacgcg
    12301 gtccgcagga tgtggaccgg gaaaggaaat tgatgcgccc gcacatttcg ttgaatggtc
    12361 tgtggaagtt ctgccccgcg tttgacgcaa tcagtgcaga tcagcgatgg ctcgactccg
    12421 attttgaccc gaagaatccc gatcttacgc cggttaccgc aggtgcggcg aggggatgga
    12481 tcaagcccga ttatgacgac agccggtggc tggatatcac ggtgccgaac agctggaacg
    12541 ccgcgatccg cgacctttgg agttacgaag ggcacggttg gtatcgtcgc acgattcaca
    12601 tcccccgcgc gtggaaagac aagcgggtgg agttcgtttc cgaaggagcg aattatcgag
    12661 tggcgctgta cgtgaacggc gaattggcgg gagaacacga gggcgggtac aacccgttca
    12721 gtattccgat ccaccgtttc ctcaaattcg gagcacgcaa tctcatcgcg gttatatgtg
    12781 acaatattcc gaaaccgtat cgctcaccgg gcggccagtt cggctggtgg aaccacggag
    12841 gcttgtatcg ggatgtttcg ctccgagtga ccgacaccac gtacattggc gacgtcaccg
    12901 ttgtcacgga cgtcaatggc tcccatgtgg acgtgggggt gacagtcgaa gttgcatgcg
    12961 aggcgggcga aatgacgacc cgtcggatcg agatcgaact cgacgacccg tcaggtaaac
    13021 cggtccccct tccctccgaa cggaacgcgc agacggtgac tctcgccggg ggaatcgcga
    13081 aagccgcgtt cacattcgct ctcgacgggg tgctgttgtg gtctcccgcc gaaccgaacc
    13141 tctacgcgct acgcgtgaaa ctgttggaag acggttgcac aacggatgaa tggagtcaca
    13201 ggatcggttt gcgcacgttc aggattgacg ggacgcgttt gctcctgaat ggcgagccgt
    13261 ttctcgtcaa gggcgtgaac cgtcacgagc agtacgccgg gggaaccatc cacacgccga
    13321 cgcacacgga agcgcagatc tctgaggatg tggacctcat caaatggctc ggtgggaacg
    13381 cgattcgaca gcattacccc aaccaccgga ggctgtacga gctttgtgac gagcgcggga
    13441 tcgtgtgcat ggtggaaaac cccctgtggc aatggggacg accgctcgtt gaaacggaca
    13501 atgcggatgc cctcgccacg gcgaagcggc agctcaccga aatcattcgg gcgctcaaga
    13561 accacgcgag tgtgttcatg tggagtgtga gcaatgagaa cctcgtgaag gcgaaatctg
    13621 acgacgcaaa ggtggttgct ctggcaaatc agaccatcga gggaaacaag gagcttgtcg
    13681 cgctggccaa aaagctggat cccacacgcc cggtagtgga ggcgagcaat gaatggccgg
    13741 gtgacgccgt cctctgcgag acagatatca gcgccgtgaa cgtgtacgtc ggtaacccgg
    13801 tgccccacgt atcaggtgca ccgaagatcg tcgagctgat gaaagccaag atgcagaaac
    13861 tccgggagga gattccgggc aagcctatcc tcgcggcgga gtttggagaa tggacggtgc
    13921 gcgggctcga gcaggattac ccgccggggg agaactacca gacggccaaa ttgaccgcgc
    13981 tctggaaggg cttcatggaa cagccagaca tcatcggcgg gttcatctgg gtgtttgccg
    14041 attacgacct ccatcggcga tttctctggg cgtacgagta tcgctgcgcg tacgggcttt
    14101 tcgacatcga gaggaagcca aaagcggcgg cgtacgccat ccgcgagctg tggacggccg
    14161 ggaacgggcg gtaagatcgc ccaaaggcga accagaagca tcgcaacaaa cagagagggc
    14221 gcggttaacc gcgccctctc tgtttcaatt tcccgctcca acctcactgc atacccatca
    14281 aaccaggcgt tcgcccttcc atcctgcggc aagcttcttc gcaagctctc cgaacgcctc
    14341 cggaacgagc ctgtccatct cccgcgcctt cgtcagattg ccaattccgg gcgtgatgca
    14401 gttcacgtcg cccacgatgt tggcggctgc gttggcccag agcacatcgt tcctgcggag
    14461 ataggaccac acgaaccccg cggagaatga atctccacat cctgtcgtat cgagcacttc
    14521 gccgaggttt gccgcaggcc atgcggtgcc gtacacggca tcacccgatc ggtacgcgac
    14581 gaccgaaccc agcggcccga gcgtaatcat tactccttgc ggacctgcgt ccaggacctc
    14641 ggcggcagcg gcaaggtatt ccttcgtgcc tttcacgggc cgaccgagaa gctgctcgat
    14701 ttcaaactcg ttcatctgga cggttgtcgc gcgggaaacg tactccctcc attccggtag
    14761 accgacgaat acccgtcgcc cttccggtgt ccagtcggct attttgttgt gaatgtcgag
    14821 atgcaggtat ttcgccctgg tgccaagccg ttggaatgat tcagcttcga gttctcgtcc
    14881 tgtaatgaaa ttgaccagga ctgcgtcgca actggcggca agttcaagct gttccggcgc
    14941 cagcggaggg ggtacgttct gcatgctctc cgtgcgctct gtaacgctct tgtagataag
    15001 ctgtacgtga gcatttttct ggtccggcat acgacgcagg ccgtccatca ccacaccacg
    15061 gtaagacgag taaaggcgaa cgacgtcttc gtagcgatcg taccccacct gagcaatcgg
    15121 aacgagctgg gtgtgttcat cgatgatgga ttccagcgcc gcgatgttgt aggtgattcc
    15181 gccgaacgat tgcttccgat cgccacggtg tgtgacgatt tcgtcgtaca cgattgttcc
    15241 aacaacagca atacgcatgt atcaccccgc actggtgaga aaaggttggc atcacaaaac
    15301 aaggagagga gcatccgggt gctcctctcc aaaacatcac tatggccggc tcagacccac
    15361 tgtttgagat ggcgcagacc cctgatgaaa ccctcttcgc gcccttgagc cccgtcttca
    15421 tgctctatgg agagaacgcc tttgtaaccg atcgcgtaca gccgctcgat gtagacaccc
    15481 cagtcgatct cgccgaatcc ggggatgaca taccgccacc accctcctgc cagatagccg
    15541 acgtgtctca gcacgtgctg tttgatctct gtatccttcg cgtgggtatg gaagatgcga
    15601 tcccggtagc gttccactgc ccccaaatag tctatcccct gccacatgag atgggacggg
    15661 tcaaaattca gcccgaaatg tttgtccggt acgacgtcga agatgaggtc ccagagcccg
    15721 aggtgctgga tgttggttgc ataccagttt tccatcgcga tcttgatgcc tttttcagcc
    15781 gcgtggtcaa gaatcggtcg gtacaccttc ggcacgacct ccttgatcgt gtcttccttg
    15841 gattttccct gcaccggcat tccggccatc ccgcacacga cgtccgtacc cagcatcgcc
    15901 gctgcatcaa tcaccttgag gtaatgctcg gatgtttccg cccgcccgtc gcatatgttc
    15961 acgtagtacg cgaggctgga gatcttcagg tttgatttcg cgaccagttc tttgacgcgt
    16021 tccgcctcgt tggcgttgaa acgggtggtg tcaatatggg cgcccgggcc cgctgttatc
    16081 tcaagaccgg cgaaaccgtt ttccgccgca aattcgatca ccttgtcaat gggttctttc
    16141 ccgaacggtg cggtcagcaa accgacgtac atcttcgtct cctttgagtc atgcagggga
    16201 accggctgga ttctggtctg ttgccaagag aaaatacctc ccgaatgtcg gggaatccaa
    16261 ctgagcgaca tgtcgccagc ttggtttgcg aaagagattc cacgttgttc atatccgtca
    16321 acggaaatcc acccgaagcg agggttttga catgcctgcc attttgacct cagaaccgca
    16381 cctttttgtc gatttgaacg aagtcgagga acgctgcaac atcacaagga acttccacac
    16441 ggcacgcaaa tatggccgcc atcctgtttt gaagcaggat gcgccatggg agaaacatcc
    16501 cggaatgact gcgtcagtca tctacgacgt ggaggaaaag gtcttcaagg cgtggtacat
    16561 ggccggtttc tatgagcccg gttccaagca cgttcagtgc tatgccgttt cggacgatgg
    16621 catccattgg aacaagcctg cgctgggcct tcacgaagct ctcggttcga aggaaaacaa
    16681 catcgtgatc ccctcatcat atcacgacgg catggatcac tgggagtcca tgctgaagga
    16741 tccgctggat tccaacccgg ctcggcggta caaggcgttg ggatggtcga gttatgactg
    16801 ggatggaccg ctcagcggga tttacaccgc cacctcaccc gacggcctga actgggaaca
    16861 cacgccagaa ccggttttcc gtttccatcc gagaccgggc cggaacgatc tcgggcccgt
    16921 gggcgacgca caggggctca tgatcgacac actgaagaaa cgctatgttg cgttcctgcg
    16981 aaccggcata tcacgctcac tcagtatcag tgaggacttc gtgcgatgga cggccccccg
    17041 tccctttctg cagtctctga acgaggagga aaccctttac aacaacgtcg gattcgtgta
    17101 cggcaaccag tatctcggct ttcttaccca ctttgaccgc aacgcgtacc atcagactca
    17161 gaccctccaa cttctcacaa gccgtgacgg cgagacctgg atgcgtccgt tcgcggaccc
    17221 tgtagtcccg accagtgatg tcggggaatg ggacagattc cagatcctgc ttaccggcgc
    17281 tccgccaatc gcggtgggcg accagctgtt catttactac cggggaacag cgaggcggca
    17341 caacaaggtg aagggcgagt ttgaacctcg catcgattcg gatcaggacc gtcgcaccat
    17401 gtcaatcggg ttagcgaccc ttcgtttgga cggttttgcg tcaattgcgg caagctatga
    17461 cggcggttac atcacgacgc gcccgacggt tctgggtggg gatgtcctct ccgtgaacgc
    17521 ggtttctgat tacggtgagg ttcgattgga aatccttgac gaaaagggag aaaccattcc
    17581 cgggtactcc gcgcaggact gcattcccgt ctcggttgac tgcgtggaag cggaagtcgc
    17641 gttccgggat cggcgatccc tctcggaaat ctccgggcgg accataaaac tccggttcca
    17701 tctgaaaaac gcccggctgt attccttccg cgctacctat tcatctcacg ggtaacccat
    17761 ggggtcgaat gatcgcgcct cgtcgcaaat gtgggtgaag atgccataat gacattcttc
    17821 gtgccattct agaattggac ggtgccgttt tggcatacaa tcgtgacgct tcgcctttac
    17881 gggcgtgccg cacattgcct ggctcggtcc tagccaaaat gataagggac tgtttttcaa
    17941 tatcttgcgg cgatgcggga aatcgctgag cagagactgg cacgtcagtt gcaacactcc
    18001 tcctgcggaa gcgagaacga acgcacaacg caaacgtgga agaaaaagga gggcactgcc
    18061 atgacactcg ttagatattt tcccggtttc gcccgtccgg tgttcgggcg tcctactgat
    18121 gaacttgcgc gctggccgag tgattggccg tttgatgctc cgctgaacgc tgagtggtat
    18181 ccgcgtgcgg atgtcgtgga aaccgacaac gcctgggaaa tttcagtgga actccctgga
    18241 attgagaagg acgcggtgaa ggtttctgtt gaaaacggcg tgctttcgat ctcgggtgag
    18301 acgaaagcgg aacacaagga cgaaggccgc ggcgtatatc ggttggagcg caggtacgga
    18361 agcttctctc gcaccttcga gcttcctgac ggcgtagacg cggaaaagat cgaagctgtg
    18421 ttcaaaaatg gtctgctgaa gctcacggtc ccgaaaacgg ccgaggtgct tccgaaaacg
    18481 atcgagatca agcacgagta aagcggtcgg gcacttcggt gcccacccga aaaaacgagg
    18541 aaccggccgg attccggtcg gttcccttca tgatagataa gtaatcgcgg cgcgatatcc
    18601 tcagaaggtc ttgcgccgcg atttattgta cgggctgagc cggaacgcgt tcttgcacaa
    18661 tcacgtacgg cagaaccaga aacgccgtcg agctgcaatg acggaatttt gcgtgggggg
    18721 gggcggatgg tcggttagaa ggtgttcttg cggggaacag ccaaggcaac gatcacatcc
    18781 atgacgttcg ttccggtagg acccgtcatt acgaggtcac ccgtcgcact cagcagcggg
    18841 tacgcgtcgt gccgccgcaa cgcggcatcg ggtgagagcc cgcgacggag tgcccgttcc
    18901 cacgtcgcac cgtccacaag tcctccggcc gcatcggtcg gaccatccgt gccatcggtc
    18961 ccgaccgagg cgacagtgat atcggatcgt ccgcggattt cgcgagcggc ggaaagggca
    19021 aactcctgat ttctcccgcc tttcccggca tcccgagaga gcgttacggt ggtttcccct
    19081 cctagaatga gggcagttgg ccgctcgccg gcaggaaccc cctctgccag ggcgcacact
    19141 acctttgcag cctcactcgc ctcgccacgg agcgacgagg tgaggacgtg tgccctgtat
    19201 cctcgtcttt ccgcttcgta cgccgcgctc ttgagcgcga tgctgttcgt tcctaccagc
    19261 aaagcgtggc cgttcgaaaa ggacccctcg tccggcttgg gcgtttccgg gtattccccc
    19321 ctgtcgcccg cttcgaggtg agagacaacc cggggaggaa gcgatccacg aatgccgagt
    19381 cggtcaataa ccccaagcgc atcgccgaac gtcgtggggt cgggaacggt tggaccggac
    19441 gcgattacgt cgagagggtc accgatcacg tcggagagaa tcagcgaaac cacctgggca
    19501 cttccgcacg cgcggaggag ttgtcctccc ttgacacgag aaagatgctt gcggacacaa
    19561 ttgagttcct caatggtggc tccacacgag agcaacaccg aggttgttgt tttcttgtcc
    19621 gcgagggtaa tcccctgcgc cggagctacc agaagggatg acgcgccgcc ggacaggagt
    19681 acgaatacta cgtcatccgg ttcgctacct gcgacgtgct gcatcaactc cagtgttgct
    19741 tccacaccct gctcgtcagg aacaggatgt cccgcttcga cgactttgat gcgacgcaga
    19801 ggtactccgt gaccgtactt cgtgatcaca atcccgctcg tgacccggtc tccaagcacc
    19861 tcctccaatg ccgccgccat gtgtgcagaa gccttgcccg caccgacgac acggatccgg
    19921 ccaatttcct ccgtacgccg gtgccaggtc ccgacagcga gttcgttcgc ggcaatccga
    19981 aacgagcgtt tcacgccctc tttggggtcc gccgcggata tcgccgccag cacgatgcgc
    20041 gtcgcgtctt cgcgaggatt ggagggagtt tctccgctca caccttgacc tcggtttccg
    20101 ggggcttcag gttcgcacgt gccgcgagga ttggcgccac cacctcgtcc agatactcat
    20161 ccacctgttc gggtgcgcgc ccgacaaaac gtttcggctc cagttcccgc gcaatctctg
    20221 cggactcaag ggggaagccc gggtcgtttg ccaggcgttc cagaagatcg ttcctgcctc
    20281 cggccttcac cgcgtctccc gcggcctgcg aatgaacacg tattgtctcg tgaagcaatt
    20341 gcctgtcgcc gcccgcccga acggcagcca tgaggaggtt ctccgttgcc atgaagggca
    20401 gttcctggcg gatgtgctgt tcaatcatcg ccgggtagac gacaagacct ttcacgatgt
    20461 ttgtcgtgag tatgagcagg gcatcggcac ccaggaaggc atgaggtatc gccagccgac
    20521 ggtttgctga gtcatccagc gtgcgctcga accattgctc cgatgcagtc attgacgcat
    20581 tctggacaac gttgagaacc cagcgggcga ggccggttgc ccgctcgctg cgcatcgggt
    20641 tccttttgta ggccattgcg gatgaaccta tctgcgaaga gccgaatggc tcttcgatct
    20701 ccttgaggtg ctggagcagg cgtatatcat tgccgaactt atggacgctc tgggcaacgc
    20761 cggagagcgc ctggaggact tgccagtcct gcttgcggga gtacgtctgc ccggtgaccg
    20821 gagccacgcg ttcaaagccc atcttcctcg ccacacgggc ttccagttcc ctcacccggg
    20881 catggtctcc gtcaaacagc tcgaggaaac ttgcctgcgt gcctgttgtg cccttaagcc
    20941 cacgaaacgc gagcctgtcg agtcggtact cgatttcgag gagatcctcc acaaaatcgt
    21001 gcagccacag tgtcgccctt ttcccgaccg tggtgagctg agccggctgg aaatgggtgt
    21061 acccaagcgc gggctgagat ctccatgtac gtgcgaaatc tgcgaggttc gtgataagcc
    21121 tgacaatccg agcccggaga agtctcaatc cctcacgaat ctgccagagc tcgctattgt
    21181 ctgtgacgta acaacttgtg gcgcccaggt gaatgatcgg tcgggcaccc gggcattgct
    21241 cgccgaatgc gtgcacgtgc gccataacgt cgtgacgaag tttgcgctcc agttcgtccg
    21301 cacgtgcgat atctatgtct tcggcgtgcg ccctcatctc cgcgagttgt tcttcgctga
    21361 tcgggagccc cagctctttc tccgcttcag cgagagcgat ccacaggcgg cgccattgtc
    21421 tgaacctcgt gaggtcggcg aagatactgg ccatttccgg actggcgtac cgcgaaacga
    21481 gcgggtccgc gtaacggggt gcggtttcat tcggcatttt tcgtcgccct cggctgggag
    21541 gtcttgctga gttccgtttt cggcggcaag gtcataaact tgcg
//
LOCUS       DAOTNG010000011        21360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2527_length_21360_cov_5.889040, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000011 DAOTNG010000000
VERSION     DAOTNG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_21360_cov_5.889040"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="P5179_01200"
     CDS             complement(<1..2335)
                     /locus_tag="P5179_01200"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRS93871.1"
                     /translation="MIHAQSTFAAILAVTSAVTSVIALYAWLQRKTPGAACFAGLMTS
                     ITVWTFTGMAEMLTASAAGKILWSKASYLGILTIPPLWVTFALTFSRFERWLTRRVQA
                     VIWGVPAALLLLVFTNEFHGLVWPSIVPLTDEPGSVLIYQHGLAIWLVMAFSYTGVVF
                     GCIWLFYAAISSPRLRKGQTVALVIAALIPVASNFVYMADLSPYPGFDLTPLSFAVSG
                     AVLAWGIFGVRLLDVVPVAHHALFSGVTDGVLVIDLEERIVGVNPAATRMFGLQSSPL
                     GKSIPEATTHVTGLEFRFENGQREHGEVTFRRGEDQCWADVRVSPLVDGRERKIGELI
                     ILRDVTARKQTEERLRLAQEATAAANRELTAAIAQAREMAERAERANAAKSEFLANMS
                     HEIRTPMNGVFGMTQLLLDTDLTREQRSYAEMLRSSAESLLSVIDEVLDFSKIEAGKL
                     ALEITEMNLRTVCEDVSAVAAVHAQAKGLEFVCDVAPDMCENVRGDPTRVRQILNNLV
                     GNAVKFTAQGEIVLSARIESQMKKALNVRFSVSDTGIGIPKEKIGMLFNPFTQADMST
                     TRRFGGTGLGLSISKRLAEMMGGQIGVASEEGKGSRFWFTAQFIRPEAVEERSPDTHA
                     QLAGRRILLVDDNLSARRAASSLIRAAGCVCEEAGSLAEARDIFAAARQNAEPYHAVL
                     LDVPLDFGDPRAAIPRIDHGFDGTPIVLLVPLDKRGRAAQVEFEGVAASVTKPFRRDD
                     LLRCLTSVTGSLKPAHPPVPRFVPPSTAKHYARVLVAE"
     gene            2490..3344
                     /locus_tag="P5179_01205"
     CDS             2490..3344
                     /locus_tag="P5179_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRS93872.1"
                     /translation="MAGSPERGSLVSYHGGHTLYDGTGRLEDFVQAAVRQGFAGLGFS
                     EHMPAPPRYPYPDQPSWDEAVRKFDRYVDTVQELKVTYRDELPLLLGIEVEYLPDEEE
                     YLTDFLARYPFDYVVGSVHYVAGIGFDHCREWYDEAAQKCGGYENLAVSYYRNVRGLL
                     ALGVTDVLGHLDLINIFSPAPISGDDVAEAEEATLAAALQWNVILDVNGRGLIKPCKQ
                     IYPRAELLRKAREMGIPATFGDDSHAPDQVGARLDEVRASMRVAGYQTVTALLPDGHQ
                     IQRVELPL"
     gene            3356..4012
                     /locus_tag="P5179_01210"
     CDS             3356..4012
                     /locus_tag="P5179_01210"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRS93873.1"
                     /translation="MQLGRRLWSSKSIRRALLGLLVGAAVCSLHPDRAVLIPFLFWSV
                     PTHSMLMVPNEPAAIAAVQMLIRKTSLYDAALLVALTAAIGAGVACVIDQTVLQWFAR
                     KDRLRGFLNHSFTGRAVEWFRVWPFGTVFAFALLPLPFFLVRFLAAAAQYPIERYTAA
                     TMLGRIPRIFLLCVIGGFWVMPPWVVVALVVAALGTASVVPRRLVRIRSTVSQSVETD
                     "
     gene            4027..5574
                     /locus_tag="P5179_01215"
     CDS             4027..5574
                     /locus_tag="P5179_01215"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS93874.1"
                     /translation="MLLYAETHYPRYWLSRIRRAEPEIVVDIPWRVEPGAGIPVLLLV
                     HHANRYPVLLESVSVTVNAGTSGCTTAEFPLHTAISTQYWWKLLTLPPLAAGTTAEIV
                     PVIRYSVKGRSREMVADNYRGTSHQPFRVHVAEEPYPLPAGWYAGDVHVHTEFTRDQI
                     EFGPPIAALATIAQAMGLHWFCATDHSYDLDDREDDFLRNDPAFPRWNRMLRDCEVPR
                     EHFCVIPGEEVSCGNAEGRNVHLLALGIHEHIPGKGDSAERPFRNRPDHSITEVIDLV
                     QRQGGLALAAHPGFTPPRIQRLVFRRGVWKPDDLAREGLAGIQAGGRFSDAGFRRGME
                     QWVRLLLAGKHVCVVAGNDSHGAFGRTRQVKIPWWSIAEDSSYVFGDVRTVVRADSLS
                     NRHLLDSIAAGRCYITNGPAIRLDVSRGTTRAGMGEELAGGNVTVHVEAESTREWGYV
                     DRIGIAVGQTGGVESMTWKKPENPALRFGVDVDVPALSEGYVRVEAHTDMRRHAYSNP
                     VWITAPA"
     gene            complement(5558..7084)
                     /locus_tag="P5179_01220"
     CDS             complement(5558..7084)
                     /locus_tag="P5179_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93875.1"
                     /translation="MEHWHRRRAVRPALLVAIVVATVPAWLAAGDDYPPIQITDLQTP
                     VERATFVVQTLFDSTNGIEDVYHAAWTIAGKLYYRSGVITSLARPRQLVTGLQACAEP
                     LLFMLPSGKPGLVWAEERTPNLSIVPGEWNLRYTADVNVAPRWFTEDAGIGGVHPDRN
                     PSVAVQGDTVLVAWERDEPPQVAYAFLSPRDIFPVVRLLTSYPYGVSRPTVAADTEGA
                     YWIAWTAETPTGGDVWVARVSRNGATSASPVRAGPEDARNCVIAVNADREAYVAWQEG
                     LSGGSIYLVRALANGRFTPAVKVSPEGTVCATPQLCFESGTIPCVSWRNVLTGDLEMV
                     RVHGTRPMGPVNLVSGVYTPRQEFVTPAQLRSRSGCVVSAFLGPDPVRGVTNLWISRG
                     KEYTPGADSDSNGGNKPAAPSPFFTRVSPNPFNGSVLLEAHIPASVAWRLEIRSIDGR
                     LVRKFEGSSVAPGPVRVVWDGGDSFGQTLGSGTYLVRWSVGLPNGEMWTHTERVTLVR
                     "
     gene            7287..7841
                     /locus_tag="P5179_01225"
     CDS             7287..7841
                     /locus_tag="P5179_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013680234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS93876.1"
                     /translation="MERGVAADGIGLVGVLADSHDNRWALEAILRRFADEGVVHVLHA
                     GDFVAPFNARCLSGISVPFTGVFGNNDGERPGLTKAFAKYGTLHVGPYAFDTRGRRII
                     LMHEPVAVDALAVSGRFDVVVYGHTHETDVRKVAWADGNGSTLILNPGEAGGWLNGRA
                     TAALLDLADLNVNVFDVPFARTSE"
     gene            7854..8681
                     /locus_tag="P5179_01230"
     CDS             7854..8681
                     /locus_tag="P5179_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS93877.1"
                     /translation="MRFAICNEMFQNWEVNDVFRLAAEIGYDGVELAPFTLADSVRDL
                     SPERRREIRQQAERAGISIVGLHWLLVSPKGLYITHPDEAIREETAAYFVDLIHCCAD
                     LGGNRMILGSPKQRNLMEGVSHERGWQWTLDFLRSIVPVAEAREVTLCFEPLAPAETN
                     FIQTAEEAIRLVQGINSPNLKIILDVKAMSGENKPIPEIIRASGPWVAHFHANDANLR
                     GPGFGDVDYAPIVAALRDIGYDGWVSVEVFDFSIDPEVTARRSLSYLKEVFGIASSH"
     gene            8718..9716
                     /locus_tag="P5179_01235"
     CDS             8718..9716
                     /locus_tag="P5179_01235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRS93878.1"
                     /translation="MPEATHTLTQFEYPVLVVGAAKLANDLRAAFESPSTKAQLRRAV
                     YVDHAPAESGESLANKIADCIDPRYQAVFLDATDPVLVDDRVLTITEIKKYCPATEIV
                     VVIGKKTEGRLVELLQAGAWYFLQTPITEASLTTIMVRVVAVREADALVRTDGLTGLF
                     NRAFFEDALRDTVARLRLDIGGQRTATAPPVSLLLADLDGLKEAYGDRHQEGEMFLRE
                     MGGVVRKRYRPTDVIARIGGDEFGALLVGMNYTLALMRAELLRKETLKIPLPEGIATR
                     PTLSVGVVTYPSYFDDPQQMYWQARRALERAKAAGGNVVFGFDATGTPRPFVELGG"
     gene            complement(9766..11646)
                     /locus_tag="P5179_01240"
     CDS             complement(9766..11646)
                     /locus_tag="P5179_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS93879.1"
                     /translation="MLEDAPLEHELHNLGNDCYRSGQFEKAVEFYCRAIEENGRMVET
                     FFNRALAYARLGEYDAALNDATYVIEQNASLHDAYYLRGRIHELRLDDRAALADYDRA
                     ISLNPDFHAAVEQRTTVVSREWTYRDLRNLRAQIAIEPGNAYLCFQFGQRLLLIGHVD
                     DAVRYLETARELGFAFGDLWGELGNAYRQSRQPVKAIAAFRQAIRVNPTDVASCAHLG
                     QLLTESGHYRDAVQIFRRALGMPGDDAATLFCGLGAALHGLRKWEEAREAFQRALDTK
                     PRLPDAAAGMGLIAWRLGDRARAAQWCRAALELSPGATDAIVLSVNLALSEGALATAM
                     EMLYELFQANSEDHRLAYVLWTVDTYARKQPGFLPDEGSAVWARRRKTRETARRVVRS
                     ALKHRNDLSFVQRDLCDELLASCPVCEEAAEALYQKLITAGNDVLAVLYREIRQPALV
                     PSAERLQGAACVARMQDERWLADVCLAYADVLVGTPEAPRVEGLARVLTRLSVRGVDH
                     AEGVRDLYRFCLEGTRAGTVHTMIGLSPRVFRLALTINKPPSILPETGEFLIRLSEWL
                     ERFERAARWVLPAREWPEVGDELAELRNNLIESVPAPEGWALASVLTNFGSIARHYVP
                     IN"
     gene            11871..15242
                     /locus_tag="P5179_01245"
     CDS             11871..15242
                     /locus_tag="P5179_01245"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRS93880.1"
                     /translation="MHEIVTGPPHALFSRLARTILEFRKPEKFARITVLTPDRQTRDV
                     VRDALPVFLAQEVPSAEPARAGLFNVRIAHLAPWAGEIARPWIEDRGLRPLPRSASLA
                     VISEAVRRTTHQTNAFAVEGAALDPRDRPSFLRAVLREIVELKKACVSPSDLQERTGA
                     RYRKEIAAIYHASEAILQSNKWVDQSDILALATKRLKTDPTAPGEDFYLLFGFLHLNE
                     LEKRCLGALAAAASRACAYVPSVPGSVAFAVAEPTIELFEATWGIARNSIPAKSGVAR
                     EIVGDLFCSDAEEVHENPAICVASASEPAQEIREAARWIWKVIEEDGLRFQDFQVIVP
                     DKSLSERLAVDVIRKAGIPVNMVFGELLSDTDAGRGLLLFLDAVESGLLRTRVMDLLS
                     VCKPRGTNPPGEQARSVNSSDWDRIARIAGVFGGIRPDRDVRSEWDDRLARYERALAW
                     RIEHQPAEGEEDAVEDVASLRREHDTTCELRQLIHELDASRDAIENASSWEGWIGAVK
                     QGWQQTAEVGSSEEVRQIERVLDEIATYDAAGKLPPAPVRRTILEEALGHTRASTTHS
                     DPHGVVLTNLTGACIRPCRALVMVGLSDGEFPRTTTRTALSVQPDDGLRHESASLSLD
                     LQKAIDYAMFAMALSAASDRVRLSYSRRTDAAAAEKLPSSALMEVIRRVIPLEESPSE
                     TPLWDEKALHRELSERGEWAVREPVPRPKAFWLNAQELILAHAAGVAGTSAGEQIAGR
                     LSRHARNGMALLRARDSFPGPYDGWFAERAVDDVLANHLSSLGTEHNPVSTRALETYA
                     QCPFRFLVTRILKTEPLPEPDIVLDFSRMDFGLVLHELLAEFFRRLINEQLFPRRPES
                     ARDLRPLFDELAEEKRRELEQEGTVPPGVIWRIRWTALQESAWAAVAWSLGDVHSWEP
                     SLVELGFGVSGGRVDATSPEDPAFLPLADGGTLAVRGIIDRVDFSREAPCVRVLDYKS
                     GRKPRTLNGLTNSGQLLQALLYARMLDQWLQEKALAREVSEAWYLYLKNPVTTDDVDS
                     VPVFDTLSFSGERLHNGQEELDRVLGVIIPCMRSGFFPPLPQDPPNGEHGTCAHCDVA
                     VACGSLSCLTSRWKRYRKDLRSACFLSLRSKGGTP"
     gene            15239..18535
                     /locus_tag="P5179_01250"
     CDS             15239..18535
                     /locus_tag="P5179_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRS93881.1"
                     /translation="MSALFDQDARDRAQRSTNETLLVEASAGTGKTTTMLGRLLFLLT
                     TPTSEGERPPRLTEIAAITFTERAAQELKDRLRARLFATVSSASPAQRGLVQQALNDL
                     EEAPIGTIHGFCARILRQYPIEAGVDVEFRVAEERAAAEIWRDVWRSWITTQFHRPDL
                     PEAVRRSVELGLTPDDLYSVAEAIRRFRMHPVSEMLGPQTAAAPDEDAIEHLRELLPA
                     SNRDSRGRTLSEKRLATIETVRAFVEALSAATTQEERVNRLLQPLPESSAAELAPLLT
                     YVDELRTRYTHNLISGIVRWLTHPEEGIIAAYARAKHDAACLDFDDLLIKARDLVRNR
                     SEVRRQLQEQFRTIIVDEFQDTDPVQAELIILLSQRISPVDDGPAAPWEESGLSPGRL
                     CVVGDPKQSIYRFRGADIETYSRVASLIGVENRCHLLTNFRSHGSIIRAANAVFGRPG
                     VFSSPDLPDEARMFIPAYQPLEPPAGMQEDAEPRVLWVESATERCDALKDARPVEAAL
                     VANAIQQVVADGWEVRDPRTGQLRPARWGDVALLCRRWAVTEELEDEFRYRRIPYRIL
                     GGKGLFEREEVVRLVSLLHALEQPHDSLSVIAALRSAFFGVSDAELALAAIADASNFD
                     YTDDRQDALPETIGRAFALLREARASLNELRPSEVIEFLFEKTGVLAAYAQQPDGEQS
                     IANLLKIVDEARRYEAENSYSFRGFCNRLRTLRLLNAEESEAELDDEAGGRVQLLTIH
                     KAKGLEFPVVFLLDLGAPLNSSQSSEKLVIVRGDGRTPPRCGLLVKKLPGGASFCTRD
                     WKEARGADRERESAEMTRLLYVALTRARDYLLLPRVLVTKNATWQSLIESASESHEWP
                     VLQPRDFRDSQSAAPAVERADVISAFETELRRFEEKRRDATEQASRPRVRIIYPSSEH
                     PDERERWADESPTPAVHGSPEDDSGRRLGTVVHRALQRLHPGNLSGVERVVKIEAEEE
                     DLNDEERARAVALVRGCFSLPVINRAYSAPQRWKEVPVTLCEDTNGVRNIIRGVCDLV
                     FTQGSELVLVDYKTDRGSPQDADNLKLKYKPQLDEYVNALNVATGLTVAEAWLCFLGL
                     DGGPIECRVV"
     gene            18640..19788
                     /locus_tag="P5179_01255"
     CDS             18640..19788
                     /locus_tag="P5179_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS93882.1"
                     /translation="MRFVLLYFTHFISFGAFVPFLPLYLRRAAGLSHVEIGWVLAVSN
                     LVSLVGLFVWGPFVDRTDKRRRTLVLGMIASAALYPAYMLTRGLPEIIGLTIVLGVFM
                     TPVIPLLDDLLLTHIRIHGGEYGRTRLWGSAAFIVGSTAAGWLLGHGVAGGQFFVFIA
                     AEIAALVVLATFSSEEINRPGAAVKDRHFWRSLTGPFIVFLVAAFLGRISAIGHYNLY
                     SLYLDKIGVSDTLKGVAWSIGVVAEIGMMFFTGRIIRRFGARWLFTLGLVGSAARFAL
                     YAVWPTPTGAIAGQVFHAFTFGAYHVGAVTLVTELAPPGRSATGQFALASLAYGLGST
                     VGSVVSGQVAQIWGYQVMYVMCAGLAVLAALVAQISAPEKGRTVFSAP"
     gene            complement(19785..21224)
                     /locus_tag="P5179_01260"
     CDS             complement(19785..21224)
                     /locus_tag="P5179_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HRS93883.1"
                     /translation="MEYTYDSWQRAWAEIYEASCETGRKRWGTLPGIAIMFHSVLDGM
                     VRFQPGRIERILSADPRLREEVGERIGTLPDEITSPADLIVAFLESFRNGQALQRMIR
                     SEAVFKWVSEHFGYDELTLGGTSANMAVTVASLGVPRVLVYANPLTKPLAQAFPSLSN
                     LLTIGPDGTVGSPRDVAQGDDVFAIHWIFEYQKDDTIRIDGQTLTAPRANRFIPSWNP
                     ANNQLRLAPGFKATFLKDADRFSHLLVSGFHILSDRYPDGTTAQECIVPVADYLRLVR
                     TRAPHMRVHCELASIAGKIVRKGVLDHILPQMDSIGLNEVELATWLEEIGAGELAGNV
                     RDQNAPEAILAGLNRLADATGVPRIHLHNFGYYMVLAEKTAGSPEGIRQGLACGACAA
                     VVRAKTGRPPKSDEVTSFVDLPLRAEAFDAMRTLAPSAKTPDFALTGVGTSGGRNLVF
                     VPTRIVERPARTVGLGDTISASAWLAEGD"
BASE COUNT         4235 a         6373 c         6473 g         4279 t
ORIGIN      
        1 cttccgcaac gagcacgcgt gcgtagtgct tcgcggtgga cgggggcaca aatcggggaa
       61 cgggcgggtg tgctggtttc aaagagcctg taaccgatgt caggcagcgg aggagatcgt
      121 ctcttcgaaa cggtttcgta actgatgcgg cgacaccctc gaattccact tgggcggccc
      181 gcccccgctt gtccaacgga accagcagaa caatcggagt tccgtcaaat ccgtgatcga
      241 tacggggaat ggccgctcgg gggtctccaa agtcgagggg aacatcaagc aacaccgcgt
      301 ggtagggttc cgcattctgg cgtgccgccg cgaagatatc ccgcgcctcc gcaaggcttc
      361 cggcttcttc gcacacgcaa ccggccgccc ggatgagaga tgaggcagct ctgcgcgcgg
      421 aaaggttgtc atccaccaga agaatgcgtc ggccggccaa ctgcgcgtgc gtgtctggcg
      481 aacgctcctc aacggcttcc ggacgaatga actgcgcggt gaaccagaag cgcgatcctt
      541 tcccttcttc gcttgcgacg cctatctgcc cgcccatcat ctctgcaagg cgcttggaaa
      601 tggacaaccc gagccctgtt cctccaaagc gccgggtcgt cgacatatcg gcctgcgtaa
      661 acgggttgaa cagcatcccg attttctctt tcgggatccc aattcccgta tcggataccg
      721 aaaaacggac gttcagggct ttcttcatct gcgattcgat tcgcgcggaa agcacgatct
      781 ccccttgtgc cgtgaacttc accgcattgc caaccagatt gttcagaatc tggcggaccc
      841 tcgtcggatc gccgcgcaca ttctcgcaca tgtcgggcgc cacgtcgcac acgaattcca
      901 gtcccttggc ctgtgcatgt accgccgcaa ctgccgacac atcctcgcac accgtgcgaa
      961 ggttcatttc agtgatttcc agcgccagtt ttcctgcctc tattttggaa aaatcgagga
     1021 cttcgtcgat gacactcagg aggctctccg cgcttgaccg cagcatctct gcgtaactgc
     1081 gttgttcgcg ggtgagatca gtatcaagca gaagctgcgt catcccgaac acgccgttca
     1141 tgggtgttcg gatctcgtgg ctcatatttg ccagaaactc gcttttcgca gcgtttgccc
     1201 gttccgcgcg ctccgccatc tcccgcgcct gggcaatagc cgcagtcagt tctctgttgg
     1261 ccgcggcggt tgcttcctgc gcaaggcgga gccgttcctc tgtctgtttg cgcgccgtca
     1321 cgtcgcgcag aatgatcagc tctccgattt tccgttcgcg gccgtcgacg agtggcgaga
     1381 cccggacgtc ggcccaacac tgatcttcgc cgcgacggaa ggttacttcg ccatgttcgc
     1441 gttgcccgtt ttcgaatctg aactccagcc cggtaacatg tgtcgtcgct tcgggaatgc
     1501 ttttcccgag cgggctggac tggagcccaa acatcctcgt cgctgccgga ttgacgccga
     1561 ctatcctctc ctcgaggtct attacaagca cgccgtctgt aacaccggag aaaagggcgt
     1621 ggtgcgcaac cggaacaacg tccagcagac gcacgccgaa gataccccac gcaagaactg
     1681 ctcccgagac cgcgaatgac aagggagtga ggtcgaagcc cggataaggg ctcaaatccg
     1741 ccatgtagac gaagttgctt gccacaggaa tcaacgcggc gatgacgagc gccacggtct
     1801 gacctttccg caaccgaggc gaactgatcg cggcgtaaaa gagccatatg cagccgaaga
     1861 cgacacccgt gtaggaaaac gccatcacca accagatcgc gagcccgtgc tggtaaatca
     1921 gcacggaacc cggttcgtcg gtaagtggca caatcgaagg ccagacaaga ccgtggaact
     1981 cattggtgaa tacgagaagc agaagcgccg ctggaacacc ccagatcact gcctgaacac
     2041 gccgggtcaa ccatctttcg aaccggctga atgtgagcgc gaacgtaacc cagagcgggg
     2101 gaatcgtgag tattccgagg taacttgctt tcgaccagag aatcttgccc gctgcggacg
     2161 ccgtcagcat ttctgccatt ccggtgaacg tccacaccgt gatagatgtc atcaaccccg
     2221 caaagcaggc tgctcccggc gttttgcgct gcaaccaggc gtacaatgcg attacggaag
     2281 tgacggcgga cgtgaccgcc agtatggccg cgaaggtgct ctgcgcatgg atcatgcagg
     2341 caaattcccg tcaaatggct gcggaacggc gcttaacgtt gcgccggttc gacttcctcg
     2401 atcgttttct gttggtaaac accgatgagg cgattatcat gtactagacg aacttcgcac
     2461 acgcagaacg aatatatgtg ggagcatttg tggcaggaag tcctgaacgt gggtcacttg
     2521 tgtcgtatca cggggggcac acgctgtacg atggaacggg gcggctcgag gattttgtcc
     2581 aggcagcggt tcggcaaggg tttgcaggat tggggttcag cgagcacatg ccggcacccc
     2641 ctcgttatcc ttaccccgat cagccttcgt gggacgaagc tgttcgaaag ttcgaccgtt
     2701 acgtcgatac ggtccaagag ctgaaggtta cgtacagaga tgaactgccg cttctgctcg
     2761 gcatagaggt cgagtacctt cccgacgaag aggagtatct cacggatttt ctcgcccggt
     2821 accccttcga ctacgtggta gggtcggtgc attatgtggc gggaatcgga ttcgatcact
     2881 gccgggagtg gtatgacgag gcggctcaga agtgcggagg ctacgagaac cttgcagttt
     2941 cgtactacag gaacgtgagg gggttactgg ccctgggtgt gacggacgta ctcgggcatc
     3001 tcgacctcat caacatattc tcccctgcgc caatttccgg ggacgatgtg gcggaagcgg
     3061 aggaggcaac actagcggca gcactgcaat ggaacgtcat ccttgacgtg aacgggcgcg
     3121 ggctcatcaa accgtgcaag cagatatatc cccgcgcgga actactcagg aaagcgcgcg
     3181 aaatggggat tccggccacc ttcggagatg attcccacgc acctgatcag gtgggggcac
     3241 ggctggacga agtgcgcgcg tccatgcgtg ttgcgggata tcaaacggta accgcattgt
     3301 tgcccgacgg gcaccagatt cagcgagtcg aattgccatt ataggagtca ccattttgca
     3361 attgggtcgc cgtctgtggt ccagcaagtc tatcagaaga gcgctgctgg ggcttcttgt
     3421 cggcgccgcg gtctgttcgc tccaccctga ccgcgcagtc ctgattcctt tcctgttctg
     3481 gtcggtcccg acgcattcga tgctgatggt tccgaatgag ccagcggcaa ttgccgccgt
     3541 ccagatgctc atcagaaaaa cctccctcta cgacgctgcg ttgctggtag cacttactgc
     3601 cgcaatcggc gcgggcgtcg cctgcgtgat tgaccagacc gtgcttcagt ggtttgcacg
     3661 caaggaccga ctccgcggct tcttgaacca ttctttcacc gggcgcgcag tagaatggtt
     3721 tcgcgtatgg ccattcggga cggttttcgc attcgccctc ctgccactgc ccttttttct
     3781 cgtccgattc ctcgccgcgg cagcgcagta cccgatagaa cggtacactg cggcgaccat
     3841 gcttggccgc attccgagga tattccttct ctgcgtcatc ggcggcttct gggtgatgcc
     3901 gccctgggtc gtggttgcgc tggttgtggc ggcactgggg accgcctccg ttgtaccgag
     3961 gcgattggtg cgcatccgaa gcaccgtctc gcaaagcgtc gaaaccgact gacgcgactc
     4021 cttcccatgc ttctctacgc agaaacacat tacccccgat actggctcag ccgcatacgc
     4081 agggctgagc cggaaatcgt ggtggatatt ccctggcgtg tggagccagg cgcggggatc
     4141 cccgttctgc ttctcgttca ccacgcgaat cgctaccctg tcctgctcga atccgtttcc
     4201 gtaaccgtca atgccggcac ttccggttgc acaaccgcag agtttcccct tcacactgca
     4261 atcagcaccc agtactggtg gaagctgctc actcttcctc ccctcgccgc cggaaccacg
     4321 gctgaaatcg tgcctgtcat ccggtattcg gtgaagggac gctcgcggga gatggtggcg
     4381 gacaactatc gtggaacatc gcaccagccg ttccgtgtgc atgtcgcaga ggagccgtac
     4441 ccccttcctg cagggtggta cgcaggtgac gtgcacgttc acaccgagtt caccagggat
     4501 caaatcgagt tcgggccacc tatcgcggcg cttgccacca tcgcgcaggc aatggggttg
     4561 cactggttct gcgcaaccga tcattcctac gatcttgacg atcgggaaga cgatttcctg
     4621 cgaaacgacc ctgcgtttcc ccgctggaac cggatgttac gggattgcga ggtcccccgg
     4681 gaacatttct gcgttattcc gggcgaggaa gtatcctgcg gcaatgcgga gggaaggaat
     4741 gtccacctgc tggccctcgg catccacgaa catattccgg ggaaggggga cagcgcggag
     4801 cgaccgttcc ggaaccgccc cgaccattcc atcacggaag taattgacct tgtgcagcgg
     4861 cagggagggt tggcgctggc cgcgcatccc ggcttcaccc cgcctcgcat tcagcgactt
     4921 gttttccggc gcggcgtgtg gaagcccgat gaccttgctc gtgaagggct ggcagggata
     4981 caggccgggg gacgcttctc cgacgcggga ttccggcgcg ggatggagca gtgggtaagg
     5041 ctcctgcttg cgggaaagca cgtctgtgtt gtggcgggga acgattccca cggggcattt
     5101 ggccgcacac gtcaggtaaa gatcccctgg tggtcgatcg cagaggactc gtcatacgtg
     5161 tttggcgacg ttcggacggt cgttcgtgcc gattcactat cgaaccgcca tctgctcgat
     5221 tcgattgccg cggggcggtg ttacataacg aacggtccgg cgattcgcct tgacgtttcc
     5281 cgcggcacca cccgcgctgg aatgggtgag gaactggccg gcggcaatgt tacggtccac
     5341 gtggaagccg aaagcacccg ggaatggggt tatgtggacc gtatcggaat cgccgtcgga
     5401 cagacgggag gcgttgagtc catgacgtgg aaaaagccgg agaacccggc cttgcgattc
     5461 ggggttgatg tggatgtgcc agcgttgagc gagggttatg tgcgcgtgga agcgcacact
     5521 gacatgcgga gacacgcgta ctccaacccc gtatggatta ccgcaccagc gtaacccgtt
     5581 cggtatgagt ccacatctcg ccgttcggca gccccacaga ccagcggacg aggtatgtgc
     5641 cgctccccag cgtctgtccg aaggagtccc cgccatccca cactacccga acagggccag
     5701 gtgcgacgga ggatccttcg aactttcgca cgagcctccc gtcaatggaa cggatctcca
     5761 gtcgccacgc aacggacgca ggaatgtggg cttccagcag aacggagcca ttgaagggat
     5821 tcggggacac ccgcgtaaaa aacggggaag gcgccgccgg tttgttgcca ccattcgaat
     5881 ctgaatcggc gccaggggtg tattccttcc ctctcgatat ccacagattc gtcacgccgc
     5941 ggacgggatc agggccaagg aaggcgctca ccacacaacc ggaacgggaa cggagttgtg
     6001 ccggtgtgac gaactcctgc cgtggtgtgt agacacccga gacgagattc acgggaccca
     6061 ttggcctggt tccgtgcacc cggaccattt ccaggtcccc agtgagtaca tttcgccagc
     6121 ttacgcaggg aatcgtgcca ctttcgaaac agagctgcgg tgttgcgcag acggtcccct
     6181 ccggggacac cttgacggcc ggtgtgaagc gcccgttggc cagcgcgcgc acgagataga
     6241 tgcttccgcc agagaggcct tcctgccatg caacgtatgc ttctcggtcc gcattgacgg
     6301 cgatgacgca gttccgtgca tcctctggtc ccgcgcgcac aggtgacgcg gatgtcgcgc
     6361 cgttcctgct cactcgcgca acccacacgt ccccccctgt tggtgtctca gccgtccatg
     6421 caatccagta cgcaccttcc gtgtcggcag cgacagtcgg gcgcgaaacc ccgtacgggt
     6481 atgacgtgag gagtcgcacg acgggaaaga tgtcccgggg cgaaaggaat gcgtaggcaa
     6541 cctgcggtgg ctcgtcccgt tcccaggcga ccagcaccgt gtctccctga actgccaccg
     6601 aaggattgcg atccgggtga actcctccga ttcccgcgtc ttcggtgaac caccgcggtg
     6661 caacgttcac atctgccgtg taccggaggt tccactcgcc gggaacgata ctgaggttgg
     6721 gggtccgctc ctctgcccag acaagacccg gtttcccgga aggaagcatg aacagcaacg
     6781 gctcagcaca ggcctgcagc cctgtcacaa gttgcctggg ccgcgccagg cttgtgatca
     6841 ccccggagcg gtagtacagt ttcccggcaa tcgtccatgc ggcgtggtag acgtcttcga
     6901 ttccgttcgt ggagtcgaac agagtctgga caacaaatgt ggcccgttcg accggtgttt
     6961 ggagatctgt gatctggatc ggggggtaat cgtcaccggc ggccaaccat gcaggcaccg
     7021 tcgccacgac aatggccacc aaaagggcag ggcgaactgc gcgcctgcga tgccaatgtt
     7081 ccacggttgc cacacctcgc cgcaatcacg ccacacaaag ccgccagaaa cggagcaata
     7141 tgacaaaatg tacccttcga agttccggaa tgcctaacgc gcaggttcgg gtgccgtatt
     7201 agcgcgtctg gacctccggt tgacgaaatc ggtgtccacg cgtaatttat gcagggttgt
     7261 ggcgatccgg cacagacgag ggtggaatgg aacgcggagt tgccgctgac ggaattgggc
     7321 tggtgggggt actcgcggac tctcacgaca acagatgggc tcttgaggcg atcctgcggc
     7381 gttttgcaga tgagggcgtc gttcatgtcc tccatgccgg tgacttcgta gcgcctttca
     7441 acgcgcgctg tctttcgggc atttcagtgc catttacggg cgttttcggg aacaacgacg
     7501 gcgaacgacc cggtttgacg aaggcgttcg cgaaatacgg gacgttacac gtcggcccgt
     7561 acgcatttga tactcggggc cgccggatta tcctcatgca cgagcccgtt gccgttgacg
     7621 ctctcgccgt atcgggcagg tttgatgtgg ttgtctacgg tcacacccac gaaacggacg
     7681 tccgcaaagt cgcgtgggct gatgggaacg gatcaacgct cattttgaac cccggcgagg
     7741 cgggggggtg gttgaacggt cgggcgacgg cagctcttct tgatctcgct gacctgaatg
     7801 tcaacgtgtt cgacgttcct ttcgcccgaa cctctgaata acaggagtat tctgtgcgtt
     7861 ttgccatttg caacgaaatg tttcagaact gggaagtgaa cgacgtattc cggcttgccg
     7921 cagagatcgg gtacgacggc gtagagctgg cgccgttcac actcgccgat tcggttcgtg
     7981 atctcagccc cgagcgccgg cgggagatcc gccagcaggc cgaacgcgct ggcatctcca
     8041 tcgttgggct ccactggctg cttgtgagcc ccaagggcct ctacattacg cacccggacg
     8101 aagcgatacg cgaagagacg gcagcctatt tcgtggacct catccattgt tgcgcggatc
     8161 tcgggggcaa cagaatgatc ctgggttcgc caaagcaacg gaacctgatg gaaggtgtct
     8221 cgcacgaacg gggctggcaa tggacgctgg atttcctccg ttccattgtg cctgtcgccg
     8281 aagcacgtga ggtaacgctt tgcttcgagc ctctcgcgcc cgctgagacc aacttcatac
     8341 agaccgccga agaggcaatt cgactcgtcc agggcatcaa cagcccgaac ctgaagatca
     8401 tactggatgt gaaggccatg tcgggcgaga acaagcccat cccggaaatc atcagggcga
     8461 gcggaccctg ggttgcccac ttccacgcga acgatgcgaa cctgcgcggg cccgggttcg
     8521 gcgatgtgga ctacgctccg atcgtcgcgg ccctgcgcga tatcgggtac gacggctggg
     8581 tttccgtcga agtgttcgat ttctcgattg atccggaagt gacagcacgt cgaagtctca
     8641 gctacttgaa agaagtcttt ggaattgcct cgtcacacta aggttgtcct ctcggaagga
     8701 aggtgaaggg ggtacttatg ccggaagcaa ctcacaccct gacgcagttc gagtacccgg
     8761 ttctcgtggt aggggcagcg aagctcgcga acgatttgag agccgcgttt gaatcgcctt
     8821 caacgaaagc gcagctccga cgcgcggtgt acgtagacca tgcgccggca gaaagcgggg
     8881 aatctctcgc gaacaagatc gccgattgta tcgatccgcg gtaccaggct gtcttcctcg
     8941 atgcaaccga cccggttctt gttgatgacc gcgtgttgac gatcacagag atcaagaaat
     9001 actgcccggc gacagaaata gtcgtggtga taggaaagaa gaccgaaggg cgtctggtcg
     9061 agcttctgca agcgggagca tggtattttc ttcagacgcc gattactgaa gcctccctca
     9121 ccacgatcat ggtgcgggtg gtcgcggttc gcgaggcaga tgcgctggtc cggacggacg
     9181 gtttgaccgg tcttttcaat cgggcgtttt tcgaggatgc gttgcgtgac acggtggcgc
     9241 gtctgcgact ggacatcggt ggtcagagaa cggccacggc cccacctgta agcctgctgc
     9301 ttgcagatct ggacggcctt aaggaagcgt acggcgatcg ccaccaggaa ggtgagatgt
     9361 ttctccgtga gatgggggga gtggtacgca agcgatatcg tcccacggat gtcatcgccc
     9421 gaatcggggg agatgaattc ggggcactcc tcgtcgggat gaactacacg ctcgctctga
     9481 tgcgagcgga gcttctccgc aaggaaaccc tcaaaatccc gttgccggag ggaatcgcca
     9541 cacgcccgac actttccgtc ggcgtagtca cctatccttc gtatttcgac gaccctcaac
     9601 agatgtactg gcaggcgcgg cgcgcgcttg agcgcgcgaa agcggcgggc ggcaacgtgg
     9661 tgtttggatt tgacgcaacc ggaaccccgc ggccgtttgt cgaactcggc ggataagacc
     9721 tggggagaag cggagtaatt cggtggcgat cggcttgagg ctggttcaat tgatcgggac
     9781 gtagtggcgg gcgatagacc cgaagttggt gagcactgag gcgagagccc agccttccgg
     9841 ggccggaaca ctctcgataa ggttgttccg cagttcggcc agttcgtccc ccacttccgg
     9901 ccattcccgc gccggaagca cccatctggc cgctcgttca aacctttcaa gccattccga
     9961 gagacggatc aggaactccc ctgtctccgg gaggattgac ggcggtttgt tgatggtcag
    10021 agcgaggcgg aacacccgtg gcgacaggcc gatcatcgtg tgcactgtcc ccgctctggt
    10081 tccctcgagg caaaaccggt acaggtcacg aaccccttct gcgtggtcca cgccacggac
    10141 actcagccgc gttagaacgc gtgcaagccc ttccacacga ggtgcttccg gcgttccgac
    10201 cagcacgtcc gcgtacgcaa ggcacacgtc cgccaaccac ctttcgtcct gcattcgcgc
    10261 aacgcacgcc gcgccctgca accgttccgc ggagggaacc agcgcgggct ggcgaatttc
    10321 ccggtagagc accgcaagca cgtcatttcc tgcagtgatc agtttctggt acagcgcctc
    10381 cgcggcctct tcgcacacgg ggcacgacgc gagcaattcg tcacaaagat cgcgctggac
    10441 gaacgagaga tcgttgcggt gtttcaacgc ggaccggacc acccgccgtg ctgtttcacg
    10501 cgtttttctc cggcgagccc acactgcgct gccttcgtca ggcaggaaac cgggttgctt
    10561 gcgagcgtac gtatcgacgg tccacaggac gtacgcgaga cggtgatctt ccgaattcgc
    10621 ctgaaacagc tcgtacagca tctccatcgc ggtggcaagt gcgccttccg acagcgcgag
    10681 attcacggac agcacaatgg cgtcagtggc ccccggagaa agctccaacg ccgccctgca
    10741 ccattgtgcc gctcgggcgc ggtcacccag acgccaagcg atcagaccca ttccggccgc
    10801 agcgtcagga agccggggtt tcgtatccag cgcacgctgg aatgcttcgc gtgcctcctc
    10861 ccattttcga agtccgtgaa gcgcggcgcc aagaccgcag aaaagcgtgg ctgcatcatc
    10921 gcccggcatt ccgagagctc gccggaagat ctgtaccgcg tcgcgataat gacccgattc
    10981 agtcaggagt tggcccaagt gcgcacatga agcgacgtcg gtcgggttaa cccggattgc
    11041 ctgacggaac gccgcgatgg ccttcacagg ctgccgtgac tggcgatacg cgttgccaag
    11101 ttcgccccag agatcaccga aggcaaaacc gagttctcga gcggtttcca ggtaacgaac
    11161 cgcgtcgtcc acatggccga tcaacagaag ccgctgaccg aattgaaaac agaggtaggc
    11221 gtttcccggc tcgattgcta tctgggcgcg aaggttacgc aaatcgcgat aggtccattc
    11281 gcgggaaaca acggtcgtgc gctgctctac tgcggcgtgg aaatcggggt tgagagaaat
    11341 cgccctgtcg taatcggcca gagcagcgcg atcgtcaagg cgcagttcgt ggatgcgccc
    11401 ccgcaggtaa taggcgtcgt gcagactcgc gttttgttcg attacgtagg ttgcatcgtt
    11461 gagagcagcg tcgtactccc cgaggcgagc atatgcgagg gctctgttga aaaacgtttc
    11521 caccatgcga ccgttttctt cgatcgcccg gcagtagaac tccactgcct tctcgaactg
    11581 gccgctgcgg tagcaatcgt tgccaaggtt gtggagttca tgttctaacg gcgcgtcttc
    11641 cagcatgatt gaacctcctg cgcgcgtcgg atcccggaaa ctcatcgggg taattgcccc
    11701 gggggacggg aaaagggatc gaatataacg tacgatgtgc attcggcagg ggacaagcgc
    11761 cgacagcctc tcgtgcgcgc ttgcaccgtt cgccgggaaa cgcgtagact tggtagaaaa
    11821 ccaccttggg cgatgagaaa acgggcacgc atttcgattc gggaacccgc atgcacgaga
    11881 tcgttactgg acctcctcac gcactctttt cgcggcttgc tcgaacgatt cttgagttcc
    11941 gcaagcctga aaagtttgcg aggatcaccg tcctgactcc ggatcgacag acgcgcgatg
    12001 tggtgcgcga tgcccttccc gttttcctgg cacaggaagt cccgtcggcg gaaccggccc
    12061 gcgcgggcct gttcaatgtg cggattgccc atctggcccc ctgggcgggc gagattgccc
    12121 gaccctggat tgaggaccgc gggctgagac cgcttccgcg ttcggcctcg cttgcagtga
    12181 tttccgaggc ggtccggcgc accacacacc agacgaacgc gtttgcagtt gagggggcgg
    12241 cgctcgaccc gcgagatcgg ccaagtttcc tgcgcgcggt gctgcgcgag atcgtcgaac
    12301 tgaaaaaggc atgcgtctcg ccatctgatc tccaggaacg caccggggcg cggtatcgca
    12361 aggaaatcgc ggcaatttac catgcgagcg aggctatcct ccaatcgaac aaatgggttg
    12421 accagagcga cattctggcc ctggcaacca agcgactgaa aacggatccc accgccccgg
    12481 gagaggattt ctacctcctt ttcgggtttc ttcaccttaa cgagttggaa aagcgctgcc
    12541 ttggcgcgct ggcggccgcc gcttcccgcg cctgcgcgta cgttccgtcg gtaccaggaa
    12601 gcgttgcgtt cgcggtggcg gaaccgacca tcgaactttt cgaggcgacg tggggcatcg
    12661 cacgaaactc gataccggcg aaatcgggcg tcgccaggga aatcgtcggc gatctgtttt
    12721 gttcggacgc ggaagaagtg cacgagaatc cggcaatctg cgttgccagt gcttcggaac
    12781 cagctcagga gatccgcgag gcagcccggt ggatctggaa agtcatcgag gaagacggct
    12841 tgaggtttca ggatttccag gtcatcgttc ctgacaaaag cctcagcgaa cggctcgccg
    12901 tggacgtgat acggaaagcg ggcattccgg tgaacatggt gtttggagaa ctgctttctg
    12961 acacagacgc cgggcggggc cttctgctgt tcctggatgc ggtggaaagc ggtctccttc
    13021 gcaccagggt gatggacctg ctcagtgtct gcaagccgcg cggtactaac ccgccaggtg
    13081 aacaagcacg gtctgtgaat tcctcagact gggaccgcat cgcccgtatc gccggagttt
    13141 ttggcggcat cagacccgac agagacgtac gcagtgagtg ggatgacagg cttgcgcgct
    13201 acgaacgtgc tctggcgtgg agaatcgaac atcaacccgc ggagggggag gaggacgcag
    13261 tcgaggacgt cgcctcgctg cgcagagagc atgacacaac gtgcgagctt cgccagttga
    13321 tccacgagct cgacgccagc cgtgatgcca ttgaaaatgc ctcctcgtgg gagggatgga
    13381 tcggtgcagt gaagcagggg tggcaacaaa ccgctgaggt cggcagcagc gaagaagtgc
    13441 ggcaaattga acgtgttctg gacgaaattg ccacgtacga tgctgcaggc aagttgccac
    13501 ccgcacctgt acgaaggacc atccttgagg aggcgttggg acacacaagg gcctccacca
    13561 cccacagtga tccgcacggt gtagtgctta ccaacctgac cggtgcgtgc attcgtccat
    13621 gccgggcgct tgtcatggta ggactatcgg acggtgaatt tccgcgaacc accacacgca
    13681 cggcactttc agtccaacct gacgacggtc ttcgccacga gtccgccagc ctttcgctcg
    13741 atcttcagaa ggcgattgac tatgccatgt ttgccatggc tctttctgca gcatctgacc
    13801 gcgttcgtct cagctattcc cgccggaccg atgctgcagc ggccgagaag cttccgtcct
    13861 ctgcactgat ggaagtcatc cgcagagtca tcccgcttga ggagtcgccg tcggagacgc
    13921 cgctgtggga tgaaaaggcc cttcaccgcg agctttccga acggggagaa tgggccgtac
    13981 gagaacccgt tccgaggccg aaagcgttct ggctcaacgc gcaggaactg atacttgcac
    14041 acgccgcggg cgtcgcgggc acttctgcag gagagcaaat tgccgggcgc ctgagtcggc
    14101 acgcccgcaa cggcatggcg ctcctgcgcg cccgtgattc attccccggt ccgtacgacg
    14161 ggtggtttgc ggaacgcgcg gtggatgacg tgctggcaaa ccacctctcg tcactgggaa
    14221 ccgagcacaa tcccgtttcc acgagagcgc tggaaacgta cgcccaatgt ccgttccgct
    14281 tcctggtgac gcgaatcctg aaaactgagc cgttgcccga gcccgacatc gtccttgatt
    14341 tctcgcggat ggattttgga cttgttctcc acgagctcct tgcagagttt ttccgccgct
    14401 tgatcaacga gcagctcttt ccacggcgcc ctgaatccgc gcgggatctt cgaccgcttt
    14461 ttgatgaact cgccgaagaa aaacgacgtg agctggaaca ggagggcacg gtcccgcccg
    14521 gcgtcatctg gcgcattcgt tggaccgccc tccaggaaag cgcgtgggca gcggttgcat
    14581 ggtcactggg agacgtgcat tcatgggaac cttcactggt cgaactggga ttcggtgtgt
    14641 ctggcggccg agtggatgca acctcaccag aagatccggc atttctcccg cttgcggacg
    14701 gcggaacgtt ggctgtccgg gggataatcg accgggtgga tttctcccgc gaggcacctt
    14761 gtgtccgagt tctggactac aaatcgggcc gcaaaccccg cactctgaat ggtttgacga
    14821 actcggggca actcctccag gcgctgctgt acgcgcggat gctggaccaa tggttgcaag
    14881 aaaaagcact ggcgagagag gtcagcgagg cgtggtacct gtatctcaag aaccccgtca
    14941 cgactgacga cgtggactcg gtaccggttt tcgacacgct ctcattttcc ggtgagaggt
    15001 tacacaacgg tcaggaggaa ctggatcgag ttctcggtgt gatcatcccc tgcatgcgga
    15061 gtggtttctt ccctccgctt ccacaggatc cacccaacgg tgagcacggg acctgcgcgc
    15121 attgtgacgt tgcggtcgcc tgcggatcgc tctcgtgtct cacgagccgc tggaagaggt
    15181 accggaagga cctgcgcagc gcgtgtttcc tttctctgcg gtccaaaggc ggcaccccat
    15241 gagcgcgctg ttcgaccagg atgcacgcga ccgcgcacaa aggagtacga acgaaacact
    15301 ccttgttgag gccagcgccg gcacgggcaa aaccaccacc atgctgggca ggcttctttt
    15361 cctgctgacg acgccgacgt cagagggcga acggccgccg cggttgaccg aaatcgcagc
    15421 aatcacgttt acggagcgcg cggcgcagga actgaaggac cgcctgcgcg cgaggttgtt
    15481 tgcgacggtc agcagcgcct ctcccgctca gcggggattg gttcagcagg cgctgaatga
    15541 tcttgaagaa gcgccgattg gcaccatcca cggcttttgc gcgcgaattc tgcgccagta
    15601 ccccatcgaa gcgggagtag acgtagaatt ccgtgtcgcg gaagagcgcg cggccgcaga
    15661 aatctggcgg gatgtgtggc ggagctggat cacaacccag ttccatcggc ccgatcttcc
    15721 cgaggcggtc cgacgttccg tagagctcgg gctgacaccg gacgacctgt attccgtcgc
    15781 cgaagcgata cgccgtttcc gaatgcatcc ggtatcggaa atgctcggcc cgcaaactgc
    15841 ggcagcgccg gatgaggacg cgatcgagca cctcagggaa ttgctccccg cttcgaaccg
    15901 ggacagccgc gggagaacgc tctccgagaa aaggcttgcc acgatcgaaa ccgtgcgggc
    15961 attcgtagag gcactctccg ccgccaccac gcaagaagaa cgcgtcaatc gtctgttgca
    16021 gccgcttccg gagagttctg cagcggaact ggcgccattg ctcacgtatg tcgacgagct
    16081 gcggacgcgc tacacgcaca acctgatttc gggcatcgtt cggtggttga ctcatcccga
    16141 agaaggaatc attgcggcct acgcaagggc caaacacgac gccgcgtgcc tggatttcga
    16201 cgatttgctg ataaaggccc gtgatcttgt tcggaaccgt tctgaagtgc gccgccaact
    16261 gcaagagcag tttcgaacaa tcatcgtcga cgagtttcag gataccgacc cggttcaggc
    16321 agagctcatc atcctcctct ctcagcggat cagtccggtc gacgatggac ccgccgcccc
    16381 gtgggaagaa tccgggttgt cgccggggag actctgcgtg gtgggtgatc cgaaacagtc
    16441 gatttaccgc ttccgcggag ccgatattga gacatactcc cgtgtcgcct ccctcattgg
    16501 agttgagaac aggtgccacc tcctcacgaa tttccgttcg cacgggagca tcatacgcgc
    16561 cgcaaacgcc gtgtttggac ggcctggcgt tttctcatcg ccagacctcc ccgacgaggc
    16621 gcgaatgttc attccggcgt accaaccgct ggagccgccc gcgggaatgc aggaggacgc
    16681 ggaaccacgt gtcctgtggg tcgaatcggc caccgagcgg tgcgacgcat tgaaagacgc
    16741 ccgccctgtg gaggcggcgc tcgtcgcaaa tgcgatccaa caggtggtgg cggacgggtg
    16801 ggaggtgcgc gatccccgga cagggcagtt gcgccccgcg cgttgggggg atgttgcact
    16861 cctttgccgg cgctgggcgg ttactgagga gctggaagac gaatttcgct accggcgcat
    16921 tccctaccgc attctgggtg gaaagggcct cttcgaacgc gaggaagtgg tgcgtctcgt
    16981 ctcactgctt catgccctgg aacaacctca cgacagcctc agtgtcatcg cggcgctgag
    17041 atcagccttt ttcggcgttt cggacgcaga gctcgccctt gcggcgattg cggacgcttc
    17101 aaacttcgac tacaccgacg accggcagga tgcgctcccg gaaacgattg gacgggcttt
    17161 cgctctcctg cgtgaggccc gcgccagcct gaacgagctg cgaccttccg aagtaattga
    17221 gtttctgttc gagaaaacgg gtgttctggc tgcgtatgca cagcagcccg acggagaaca
    17281 gtcgatcgcc aatctcctga agatcgttga cgaagccagg cgctatgaag cggagaattc
    17341 ctactcgttc cgggggttct gcaaccggtt gagaacgctg cgattgttga acgcggagga
    17401 aagcgaagcc gaactggacg atgaagccgg cgggcgcgtg caacttctta ccatccacaa
    17461 ggcgaaaggg ctggagttcc cggtggtgtt cctcctcgac ctgggggcgc ctttgaacag
    17521 ctcgcagagc agtgaaaagc tggtaatagt gcgcggggac ggcagaacac ccccgcggtg
    17581 tggcctgctc gtgaaaaagc ttccgggcgg agcctctttc tgcacgcgtg actggaagga
    17641 ggcccggggg gcggatcgag aacgagaatc ggcggagatg acccggctgc tctacgttgc
    17701 gttgacaagg gcacgcgact acctgctgct tccgagggtg ctggtcacaa agaacgcaac
    17761 ctggcagagc ctcatcgagt ctgcgtcgga atcacacgag tggccggtct tgcagccgcg
    17821 cgattttcgg gattcgcaat cggccgcgcc cgccgttgaa cgcgctgatg ttatctcggc
    17881 ctttgagacg gaactgcgga gatttgaaga gaaacgcaga gacgcgacag aacaggcgtc
    17941 gcgacccaga gtaagaatca tctatccatc ttctgaacac cccgatgagc gggaacggtg
    18001 ggccgatgaa tcgccgactc ccgcggtaca cggctctccc gaagacgatt cgggccgccg
    18061 gttgggaacg gtggttcacc gggccctgca gcgattgcac ccgggaaacc tttcaggcgt
    18121 cgagcgtgtg gtgaagatcg aagcggagga agaagatctg aatgacgagg aacgggccag
    18181 ggcggtagcg cttgtgcgcg gttgtttctc gcttcctgtg atcaatcgtg catacagcgc
    18241 accgcaacgc tggaaagaag taccggtaac actctgcgaa gacacgaacg gcgtaaggaa
    18301 tatcatacgc ggggtctgcg acctcgtatt tacacagggt agcgaactcg tcctggtgga
    18361 ttacaagacg gacaggggtt ccccgcagga cgcagacaac ctcaagttga agtacaaacc
    18421 acaactggat gaatacgtca acgcgctcaa cgtcgccaca ggcctcacag tagccgaagc
    18481 atggctctgc tttctcgggc tcgacggcgg gcccatagaa tgccgcgtcg tctgagtccc
    18541 tttgcgccgc gggtgaagac gcgttatcgt cattcttcca caatggatct gcgttcgtct
    18601 tcgtgactca cacctctgcg ccggccacta tctgacttca tgcgattcgt cctgctctat
    18661 ttcacccatt tcatctcctt tggcgcattc gttccgtttc tcccgctcta tctgagacgc
    18721 gctgccgggc tttcacacgt agaaatcggg tgggtgctcg cagtatcaaa tctggtaagc
    18781 ctggtaggat tgttcgtctg gggacccttc gttgacagaa cggacaaacg gagacgtacc
    18841 ctcgtcctgg gtatgatcgc cagcgcggca ctgtaccccg cgtacatgct tacacggggc
    18901 ctcccggaga tcatcggact cacaatcgtt ctcggcgtat tcatgacgcc cgtcatcccg
    18961 ttgctcgatg atctcctgct cacccacatc cggatccacg ggggagaata tggccgaacc
    19021 cgcttgtggg gttccgcagc gttcatcgtc ggaagtactg cagcgggctg gctgctgggg
    19081 cacggagttg ccggaggcca gttctttgtg ttcatcgccg cggaaatcgc cgcgcttgtt
    19141 gttcttgcaa cgttttccag cgaagaaatc aaccgccctg gcgccgccgt aaaggaccgg
    19201 cacttctggc gttctctgac cggacctttc attgtgtttc ttgtagccgc gttcctggga
    19261 agaatatcgg cgatcggcca ttacaacctc tacagcctct acctcgacaa gatcggcgtc
    19321 agcgatacgc tgaaaggcgt tgcgtggtct atcggcgttg ttgccgaaat cggcatgatg
    19381 ttctttaccg gcaggattat tcggcgcttc ggcgcgcgat ggctgttcac gcttgggctg
    19441 gtgggttcag cggcccggtt tgcactttac gcggtctggc cgaccccgac cggggcaatc
    19501 gcgggacagg tgttccatgc attcacgttc ggcgcatacc acgtcggagc agtcactctt
    19561 gtaacggaac tggcgccacc cggccgttcc gcgaccgggc agtttgcgct ggcctccctc
    19621 gcgtacgggc ttggttccac ggtgggaagc gtggtcagtg gccaggtagc ccagatatgg
    19681 ggatatcagg tgatgtacgt catgtgtgcg ggtcttgccg tcctcgccgc tcttgtcgct
    19741 cagatcagcg ctcccgagaa gggacgcacc gttttttctg ctccttagtc cccctccgca
    19801 agccatgcgc ttgccgaaat cgtgtcgccg agccccacgg tacgcgccgg gcgttctacg
    19861 attcttgtcg gtacaaacac cagattccgt cctccgctgg ttcccacccc cgtcaacgcg
    19921 aaatccggcg tcttcgcaga cggcgcgagg gtccgcatcg cgtcgaatgc ctccgcccgc
    19981 aaaggaaggt ccacgaaact ggtcacctcg tctgacttgg gagggcgccc cgtcttggcc
    20041 ctcaccacgg cggcgcacgc tccacaagca aggccctgac ggattccttc cggcgaccct
    20101 gcagtttttt ccgcaagaac catgtagtac ccgaagttgt gcagatggat gcgcggaaca
    20161 ccggtggcat cggcgagtct gttaagtccc gcaaggattg cctccggcgc gttctgatcg
    20221 cgtacgttcc ccgcgagctc cccggcgccg atttcctcca gccacgttgc cagttccact
    20281 tcattcagcc cgatgctgtc catctgagga aggatgtggt cgaggacccc ttttcgaacg
    20341 attttgccgg cgatagaagc aagctcgcag tgtacgcgca tgtggggagc tcgcgttcgc
    20401 accaggcgca agtaatccgc caccgggaca atgcactcct gtgccgtggt gccatccgga
    20461 tagcggtcgc tcaggatgtg aaaccctgag accagcaggt gggaaaatcg gtccgcgtcc
    20521 ttcagaaacg tggctttgaa accgggggcc agtcgaagct ggttgtttgc cgggttccac
    20581 gagggaatga acctgttcgc cctcggggcg gtcagtgtct gcccgtcaat gcgtatcgtg
    20641 tcatccttct gatactcgaa aatccagtgt atggcaaaca cgtcgtcgcc ctgagccacg
    20701 tcgcgcggcg agccaacggt gccgtcaggg ccgattgtca ggagattgga gagggaagga
    20761 aacgcttgcg caaggggttt tgtcagcggg ttggcataga caagcacgcg gggcaccccc
    20821 agcgaggcaa ccgttaccgc catgtttgca gaggttcccc cgagcgtgag ttcgtcgtac
    20881 ccgaagtgct ctgagaccca tttgaacacc gcttccgaac ggatcatcct ctgaagcgcc
    20941 tgcccgttcc ggaacgattc aaggaatgcg acaatcagat cggcggggga ggtgatctcg
    21001 tcggggaggg tgccgattcg ttcccccact tcttcacgca gccgaggatc cgctgacaga
    21061 atccgctcga tcctgcccgg ttggaatcgc accattccgt ccagaacgga gtggaacatg
    21121 atggcgatgc cgggaagtgt tccccacctt tttctcccgg tttcgcaact ggcctcgtag
    21181 atctccgccc aggcgcgttg ccagctatcg tacgtgtact ccatcggctt cccgtcccct
    21241 caaacaaaca gaaagagatg gcttccagat tcgggaaacc acctctccca gcagctcaaa
    21301 gcctggtcag tgcgtcaatt acgaggaaag tctacctcgg gcgacgcagt tcttccttca
//
LOCUS       DAOTNG010000012        20730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2636_length_20730_cov_5.622975, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000012 DAOTNG010000000
VERSION     DAOTNG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_20730_cov_5.622975"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="P5179_01265"
     CDS             <1..1312
                     /locus_tag="P5179_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93884.1"
                     /translation="PLFTPGLQIALDMWRYWRHTGDEQFLAETAYPVMREACRFYLGM
                     LKEESDGLFHLSASHPYEHASGYMVRDCLTDMAHIRTAFPATAAVAERLGEREFAERL
                     RDVAARLPRVETLPLPERRYRDTPGGPEFTDGWAKGLKCPSDRILCVGRRVEDGRPVH
                     FRAVEEGTGSDHLFPGSDESLVFPSGALGLRDKGSELFEAARTTALCNWEEIMGWSVT
                     PIVFARLGMSDLAEEALRRHIIHFQHFPQGLWNYQSSQSGSATDFATTFRVHDRENGR
                     DEFPFLRQPHTHFGLEPGAVVQTTVNEMFLQSYDGVIRVCPSVPQDWDGAFSLWAEGG
                     FRVSARVLRGRVPRFAVESMRGEECRIVLPYDGAWEVTDGISDATLDVNLEGDMLTFP
                     TATGGVYMISDGNEAPSPRPLLSGCAAMHAKEREGRWIGIPRMW"
     gene            complement(1400..1684)
                     /locus_tag="P5179_01270"
     CDS             complement(1400..1684)
                     /locus_tag="P5179_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS93885.1"
                     /translation="MSREELIAKAASEAGVSKKAAGEVLSALLGGITAALKSGDKVSL
                     VGFGTFAVGQRKARVGKLPGTKKEIKIPAAKVPKFKAGKNLKEAVAGKKK"
     gene            complement(1930..4650)
                     /locus_tag="P5179_01275"
     CDS             complement(1930..4650)
                     /locus_tag="P5179_01275"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRS93886.1"
                     /translation="MCEIAVLLILWLPAPLRAQLPSTATGERFATFGGFPDSVSTDSV
                     RYAADEIEYDLDTRVITLLGNARVSAAGMSLTAHRIRFLTREDLLIAEGHAISPDSII
                     GRPEFSSGQEAFSGARMTYNLRTRRGLVEEGHSEVTEGIYGGSVVKRTGERQVDVLNG
                     VYTTCDDNPPHYQFEARQMRVLVGDKVIAKPIVFKVADVPFFWLPFGVFFVDKDRRSG
                     FIAPRMGENRYAGRFMRGLGYYVAPNDYVGGQAVLDIDERIGYEWRLRTDYALRYRFQ
                     GSIASDYSKYWDTPSQIGRRIWTVTAQHRQELTPRSSVAANINFTSTSSPYVTSSSTQ
                     ASVLRQDFTSTVGYTQTWESGYSLRVDARRSQNLQNRQLDVMLPALSISSGQRYFFPE
                     PPPQRGPRAAAAPRQEPAWFRRLGYSWSWSGTNSQRRSPSDRTIFDTWETTEQRGDSV
                     IVYLLTITSTSYINSLYDGTYVVAVENGERIGEGYVQRTSDSNALVLISRSAGLPDGY
                     AIMSGNSLRVKEPDIAQEVLWTRKRILDGYTQTTSQNVGLTLPLPTPRWLTLVPTFNW
                     SASWKSRPDELNANDPQTQQTISGGVSSNATFYGVFPVHLGPVSAVRHVVTPSASFNY
                     VFSSRTRGGTYVFGGRSLGGDTSRIVDLGLRNVFQVKARTSGKERVLDQLLSVSTNAR
                     YNRDAKGRRWSDPQTSALFQPNNFISLRLSTVHTLYRAEFDSTSARYVDHLRWTRPEL
                     RSITLSTSLRMAGGGRSPSALGNEGFVVQRQETFGGNPRVPPRGAKQGDRTTADWGAW
                     QMNIRHTYQWARKEPGYTRAPEPVNQADISAEFVPWPNWRVNATTTYDVRRNRRDGDA
                     LTLVRRLHCWEAQLDWTLRGPFAGYYFRIYLLDIPDIKIESASETMRYAR"
     gene            complement(4715..5134)
                     /locus_tag="P5179_01280"
     CDS             complement(4715..5134)
                     /locus_tag="P5179_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRS93887.1"
                     /translation="MFSSTTFTVPTRRKNEMIDITGAVFDIVRQHGIKSGVCTVFVPH
                     TTAAVTINENADPDVPRDILVALERIVPRDGDYAHSEGNSPAHLKALLTGSSVTVAVE
                     TGNIQLGTWQGIFLCEFDGPRNRQVRVSVLGESAASS"
     gene            complement(5146..5529)
                     /locus_tag="P5179_01285"
     CDS             complement(5146..5529)
                     /locus_tag="P5179_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93888.1"
                     /translation="MLVVRAILLWIAMIPLAILNGTVRVYWINPVIGEHAGHIVSSVT
                     LSAIVFLLTWWFMRNAIPGPREAFLVGAGWVICTVLFEFVFGHYVMNHPWEKLLADYN
                     VFRGRTWVLVLLAEFFSPVLALRLR"
     gene            complement(5561..6556)
                     /locus_tag="P5179_01290"
     CDS             complement(5561..6556)
                     /locus_tag="P5179_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS93889.1"
                     /translation="MDDTNITSLKTGFDATMATARAFMESRAILTAVELDLFAAVCEG
                     SSASSVAHKIGTDPRATEMLMNVLVTQGLLKKAEDRYALTPVAEKYLTSTSPDSARAA
                     IMHTVGLWNSWSTLTAAVRAGTSVAKRRRNAEETEAFIAAMHRNSLERASVVAGALGI
                     ARYENMLDVGGGSGGYSITFARMNPALRSVVFDLGPVTRIARRHIKDAGVADRVSVKR
                     GDFHSDPFGNGFDLVFLSAICHMNGPEENRSLFRKAFAALQPGGDLVIHDHILNADKT
                     APKAGVLFALNMLVNTAAGNSYSEEEYHAWLKETGFSRIRSVPLPGATGLVVGTR"
     gene            complement(6700..9843)
                     /locus_tag="P5179_01295"
     CDS             complement(6700..9843)
                     /locus_tag="P5179_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS93890.1"
                     /translation="MNLPRLAIRHHVTTFMAYLLALGFGLFSLSRLKLDLYPDISFPM
                     IMVLTQYRGAGPFDMESVVTEPIESAVSSVEGIKKVTSSSRNGMSVISLEFDWDTDMD
                     MAQMNVSRALDRVTTLPEDVTKPRLFKFSTKMMPIMFMGVRSPVLNQASLRKVCEDYV
                     VPRMKRVEGVASVDVVGGLARQINVEIDPRALASYGFGIETVTAALQRSNMQVPGGSI
                     RDGRTAFAVRTLGEFLSVEQIRDVMLATKNGQVIRLRDVADVSDGFQEVLGEARIDGE
                     SGVMLIVRKQSDANTVQTVRAVKAVLPEITQSVPGNLSLTTVFDQAQYIQDSLGNLSS
                     NALQAFGLTILVLLVFLRNIRSSLIVGISIPVSMVLTFSVMDAAGVTFNVISMAGLAL
                     AVGMLVDNSIVVLENIFRLHELGYDRVEAAEKGSNQVAMAITASTLTTIVVFLPVLFV
                     PGIAGVMFNDMSLTICFALSVSIFVALTAVPSLAAGFLARQHPPRTRFLLRMYEDIGK
                     GLKWLETRYSVVLKWALGHRKTVVFTAAGLFAASIPLFMSIGVNFMEHPDDGRIRCSV
                     QHAVGTDLPTTMATLNMMEEIIKKEVPERTTVYVNAGASGGIVAALQGLDTHSGEIQV
                     KLVPLQDRDRSKSEIQDALRARFDKIPGIKYNFQQDSGPGMGGADIEVRFFGDDLDVI
                     GNLADSTVAIAKKIPGAVDARSNLDRGAPELQIRLDRDRLQAVGLTAGQVSAAVSTAI
                     QGATATRYREAGEEYNIFVRFAEEFRRSPEQLENLIIATPAGALIPLRQVADVRREVA
                     PIAIFRENQGRVATVSIGVSGRDLGSVTKDVEKAISKLNIPNDVIMEVGGGAEDLRES
                     FRYLGLAILAGIVLVYMVMASQFESLVDPLIIGTTVPLSFIGVAIALAITGTTVSVMA
                     LLGMLMLVGIVVNNGIVLVDFTNQMMRLEHKELFEAAYEAGRTRLRPVLMTALTTTMG
                     MFPLSLGLGESGESWAPLARAVMGGLLFATLMTLIVVPVVYTLFETMAIRFRAWRDRR
                     TARKHPELVVEEGAQLM"
     gene            complement(9862..11028)
                     /locus_tag="P5179_01300"
     CDS             complement(9862..11028)
                     /locus_tag="P5179_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS93891.1"
                     /translation="MEGRIRNHISTHTGDRRWTSALALTAFLAIVAIGSSGCGEKEQQ
                     ADTAEQALPVTVHAPYREDAIRVISANGSLVADKQVTIISPVSGRIVSKRVDLGSRVA
                     ANQVLFVVDYSSLDAAVQQAQAAVSSAEQQAANLATEFARVQRLYAEGGASQSQYDAI
                     RTQKAAADEGVKQARAMLQQAVIRRNDAEVRAPFSGLVGKVFVEVGDMVGPGVPLAVV
                     TEPAPLIAKIQVPERDFGTLRIGQSATVQVASYPNEAFSGTIRQISPILDAMTRMADV
                     EILLPNRDQRLKAGMYARVEIEADRRPNALMVPSNAVLQEASLVAGSTVSNITRTYYL
                     FVAEGERAVRRNVTLGYTTGDRVEITSGIRPDDKIIVQGHSFLQNGQRVSPASE"
     gene            complement(11040..12383)
                     /locus_tag="P5179_01305"
     CDS             complement(11040..12383)
                     /locus_tag="P5179_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS93892.1"
                     /translation="MLESIGRTGKVCAFWIVCSMALVSGAGARGISLREAEQMALERN
                     PQIAGAKASLDSARAGITRARATILPSLAATAGYTWNAELPVVVMPAPVGAVSIGEKH
                     NYSLALTARQPVFLGFAGVTGVRMAETALQSSQLRLDATTQTVIASVREAYLAAVLSR
                     MLVTVNEQAVAQAESSLALVQRRADVGSASRFDLLRARVQLATLRPNLTSARSNRDLA
                     DGRLRLAIGVGERDAITPSDTLAVFASEWAKVPLDSLRRIAREQRTEIRQLDIGEQIS
                     NDAVTLSRSAYYPTVAAFATSQWEAQRPSLSVSSGDFRRVSMLGLQLNWTFWDSWKTP
                     ATVQMARVGVRQLNLFRQLATDGISLEVEAAFHGLHEAELNLATGLETVNQAAEALRL
                     ASVMYSEGGNTQLDVISAQLALTSARTQYAQALYNYHVAHIRMEKALGLTRVSQD"
     gene            complement(12447..13049)
                     /locus_tag="P5179_01310"
     CDS             complement(12447..13049)
                     /locus_tag="P5179_01310"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS93893.1"
                     /translation="MEPTSHPAVGKETRDRIFAAAAKLFAARGYGSVSMREIADAAGV
                     SKPMLYYYFENKAGLCRALLEEGFQNLSQTWDEIARLDIPLIERFQRVVRAQFRHVRE
                     NPDLVRFHINFLLGPDEAGLAAEFRREHKPIVNILLRMFAEGQASGLFRPDINPSVAI
                     NVFSGAVNIHVGQSILLGGPPLDDDLADQLTQLYLHGVCA"
     gene            13195..15150
                     /gene="uvrC"
                     /locus_tag="P5179_01315"
     CDS             13195..15150
                     /gene="uvrC"
                     /locus_tag="P5179_01315"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRS93894.1"
                     /translation="MKANQARVTGPVAMANTQKEAAARAEDLRQKVSLLPRKPGVYIF
                     RDSSGEIIYVGKAKVLANRVRTYFADSYDDGRLQIHALVRSIADLEVIATSSETEALV
                     LEATLVKKHAPRYNIRLKDDKKYPYLLLTNEAFPRLVFTRSVRRGEGRYFGPYTDTRA
                     LRRTQELLHRLFPLRTCTSPLPSPSITRVCLQYEIKRCKGPCVGLQTQEEYATVTNQA
                     ERFLRGQISSVVRELEQEMDLASERLQFERAAELRDRLNDIRTTAEKQSLADARGEDR
                     DVVALTRDDTEAVAVVLEIREGKLIARKDYSLSVGLDEPVEAIMGAFLRQYYLDAMAI
                     PRELAVMCEPEDAHALEELLGHQRGGIVRLQTPRRGEKTRLVDMARQNAEMLLAERRL
                     RREKFRGRVPHSVRALERDLRLPRPPRRIACFDISHIQGTDTVGSLVVFQDGRPLKNA
                     YRMFQIRGVSGSVGESDDFAAIREVVERAFSRLAKDTEEARPDLVIVDGGKGQLSSAK
                     AVLDSLGFGDQPVIGLAKRLEEVFLPGCSEPQTIPRTSSGLKLLQQVRDEAHRFAIGY
                     HRKKRGRAMSVSALDEVPGIGIARKRALLRKFRSLASLLAAPEEEIAAVEGIGPVLAA
                     TIRAVLLDGRTGGRNGAQSPPLKVQTS"
     gene            15594..15881
                     /locus_tag="P5179_01320"
                     /pseudo
     CDS             15594..15881
                     /locus_tag="P5179_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
     gene            15951..17120
                     /locus_tag="P5179_01325"
     CDS             15951..17120
                     /locus_tag="P5179_01325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS93895.1"
                     /translation="MNRLGTENAFEVLAVINRLRAEGRDIVSFAIGEPDFPSPSNVKR
                     AGCSAIMEDKTHYGESAGLRELREAIAAHIRKTRGVDAGPENVVVTPGAKPILFNTIM
                     TLVDPGDEVMYPNPGFPIYESLIDFVGAKGVPVPLWESLDFNFDPEEFRRLVTKKTRL
                     VILNTPQNPTGGILTRDALQVVAEMAQERDFWVLSDEVYSQMAYDGEFLSIASLPGMA
                     ERTVVLDGFSKTYSMTGWRLGYGVMPADVARQEARIETNLNSCTATFTQWAGLEALTG
                     PQDESLAMIAEFKRRRSLIVDGLNRIPGFSCRKPAGAFYAFPNVTEACRMLNKSDSKA
                     LQEYLLFEAGVAVLPRTSFGRRNAGEKDEYVRFSYATSAPLIEEGLERIRKAMEG"
     gene            17235..18245
                     /locus_tag="P5179_01330"
     CDS             17235..18245
                     /locus_tag="P5179_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS93896.1"
                     /translation="MPRPVTLFTGQWADLKLADLAPKAKGFGYDGLELACWGDHFNVK
                     EAAGSMQYCDEKKKLLAKHGLKVWSISNHLAGQLVCDPNDERANGFAPPELANKPKKI
                     RDWAIDEMKLTGVAAKNLGVSVVNGFTGSGIWHAFYSFPPWTPGMVEAGYKDFARKWK
                     PILDSFADNGIKFGLEVHPTEIAYDIYTAQAALKAVKNHPAFGFNFDPSHLHWQGVSP
                     SEFIHTFGDRIFHVHMKDAIVKLDGKGSILGSHLNFGDYRRAWDFRSVGRGTVNFEEI
                     IRALNDIGYQGPLSVEWEDSGMDREHGAKESCEFVKKLDFAPSSVAFDAAFEEKGKTA
                     KA"
     gene            18531..19877
                     /locus_tag="P5179_01335"
     CDS             18531..19877
                     /locus_tag="P5179_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS93897.1"
                     /translation="MTKRFLVHPFVMLGLGALLFVGAGCESVGLRSMKIYIQQRNWEK
                     ALEQGNIALAENPNDAEAWFAMAQVAAQVDSFDLMLRAIERTESLTNKHAADFKLMRE
                     AKYGSAFNMGVERYNANDMAGASRFMDIAISIDSTRANAYRLKGMIAQRAGNTQETLQ
                     YFDRAYRADTTDVELARVYAQLLDAMGKRNEALSVAHRLYTARPGDKKTVALYLQLLT
                     EAGRFEEALNVVNTALASDPNNAEYNMRAGVLWMQRAQQDVADSTAIVRDLETAAPYF
                     EAAVEADTANTDAAYNLAVCYARLNRRGDAINVMKRLLSVRPNDYQARIQLGTLYVRE
                     ENPDAAEQEFLAVVESIGEPTNAEDRAVASRAYRYLSTIYTYRGAVKQGEADRLRERA
                     QELINESAMARQRTVRQQKEREAQALLQQAEPLATEAQALNQRSVEYDRLSNQYAN"
     gene            complement(19946..20323)
                     /gene="hisI"
                     /locus_tag="P5179_01340"
     CDS             complement(19946..20323)
                     /gene="hisI"
                     /locus_tag="P5179_01340"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRS93898.1"
                     /translation="MLDQVKFDDRGLVTAIAQDADNGEILMLAYMNRESLEATLRTGI
                     MTYWSRSRGKLWVKGETSGHTQQVVEMRIDCDGDALLFKVRQKGGACHTGFRSCFYRK
                     LTPDGLVEDGDRVFQAQAVYGTK"
     gene            complement(20338..>20730)
                     /locus_tag="P5179_01345"
     CDS             complement(20338..>20730)
                     /locus_tag="P5179_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRS93899.1"
                     /translation="VPVRPWILLCTLCLALLISLGKRRGEALLLGENAAKHRRILGEY
                     PAPFLDVLIVIVAGMTLITYCLFTFHSGHSEKLMLTIPFVLYGVFRYLYLLYVAGSTN
                     APEVLILNDKPLAAASILWALLSAALFY"
BASE COUNT         4212 a         5963 c         6335 g         4220 t
ORIGIN      
        1 gccgttgttc acgccgggcc ttcagattgc gctcgacatg tggcggtact ggcggcatac
       61 gggcgatgag cagttcctcg cagagacggc gtacccggtg atgcgcgaag cgtgcaggtt
      121 ctatctgggt atgttgaagg aggagtcgga tgggttgttt cacctttcgg cgagtcatcc
      181 gtatgaacac gcaagcgggt acatggtgcg tgattgcctg acagacatgg cacacatacg
      241 taccgcattt ccggcaactg cggccgtagc ggagcgcctt ggtgagaggg aattcgcgga
      301 gcggcttcgg gacgtcgcgg cgcgacttcc gcgcgttgaa acgctgccgt tacccgaaag
      361 acgctaccga gacactcccg ggggtccgga attcactgat gggtgggcga aggggctgaa
      421 atgcccctcg gacaggattc tgtgcgtggg acgcagggtg gaagacggtc gtccggtgca
      481 ttttcgcgca gtggaagaag ggacgggttc ggaccatctg tttccggggt cggatgagtc
      541 gctggtgttc ccatccggag cgctcgggct cagagacaaa gggagcgaac ttttcgaggc
      601 ggcccgtaca accgcgctct gcaactggga ggaaatcatg gggtggagcg tgaccccgat
      661 cgtgtttgcg aggctgggga tgtccgacct ggctgaggag gcgctccgaa ggcacattat
      721 ccattttcag cacttcccgc aggggctgtg gaactaccag agcagtcaat ccggttcggc
      781 gaccgatttt gccacgacct tccgtgtgca tgatcgggag aatgggcgcg atgagttccc
      841 gttcctccgg caaccccata cccatttcgg gcttgaaccg ggagcggtgg ttcagactac
      901 ggtcaatgag atgtttctgc agagctacga tggcgttatc cgggtgtgtc cgtcggttcc
      961 gcaggattgg gacggggcgt tttcgctatg ggcggaagga ggatttcgcg tttccgcgag
     1021 ggttctgcgc gggagggtgc cacgatttgc ggtggagagc atgcgcgggg aggaatgccg
     1081 catcgttctg ccgtacgacg gtgcgtggga agtaactgac gggatctctg acgcgaccct
     1141 cgatgtgaat ctggaaggcg acatgctgac atttccaact gccacgggtg gggtttacat
     1201 gatcagcgat ggcaatgaag cgccgagtcc gaggccgttg ctttccgggt gcgccgctat
     1261 gcacgcgaag gagcgagagg gtcgctggat aggaattccg cggatgtggt gatgcgcggg
     1321 ttgggaccgc ccggaattac aaaagccctg ccttatggag acagggcttt tgtatacatc
     1381 ggaccaagcc gggcaggggt tattttttct tgccagccac agcctctttg aggttcttgc
     1441 cagccttgaa cttcggaacc ttcgccgccg gaatcttgat ttccttcttc gttccgggaa
     1501 gcttgcctac ccgtgctttg cgctggccga cggcaaacgt gccgaagcca accagggaca
     1561 ccttgtcgcc gcttttgaga gcggcggtga tcccgcccaa caacgcgctc aacacctcgc
     1621 cggcagcctt cttggacaca ccagcttcgc tcgccgcttt tgcgatcagt tcttcacggg
     1681 acattggttc accccctacc gtgtagatgg ttcatttgcg ttcccgatga gtaaaataga
     1741 acgtcaggag cgatttgtca acacaggagt atcgattttt cggtgatttt tcaaggtttt
     1801 ttttcatccg agcgcggctg ggtcgcgttg acatgtcgtt agatgctgtt aatattaaca
     1861 tttacgttaa gcttaaaaat cccgcttctc ggaagcgttt tttcggtttt ttttgtgctc
     1921 ccgtggcgtt catcgggcgt accgcatggt ttcactggcc gattcgatct tgatatcggg
     1981 gatgtcaagg aggtaaatgc ggaaatagta tccggcaaag ggacccctga gcgtccagtc
     2041 gagttgcgct tcccagcagt gcaggcgtct gacgagagtg agggcatcac cgtctctccg
     2101 gtttctgcgc acgtcgtatg ttgtggttgc gttgacccgc cagttgggcc aggggacgaa
     2161 ttccgcggag atatccgcct ggttgacggg ttcgggcgct ctcgtgtatc caggttcctt
     2221 cctcgcccat tgataggtgt ggcgaatgtt catctgccac gcgccccagt cggccgtggt
     2281 gcggtccccc tgttttgcgc cgcggggcgg aacacgggga ttgcctccga aggtttcctg
     2341 cctttgcacg acaaagcctt cgtttccaag cgcgcttgga cttcttcccc ctcccgccat
     2401 tcgcagcgag gtgctcaggg tgatgctcct caactccgga cgcgtccagc gcaggtgatc
     2461 cacgtagcgc gccgaggttg aatcgaactc ggcgcgatag agcgtgtgaa cggttgaaag
     2521 gcgaagggag atgaagttgt taggctggaa cagcgcgctc gtttgcgggt cgctccaccg
     2581 cctgcccttc gcgtctctgt tgtaccgggc gttcgtggac acggacagaa gctgatcgag
     2641 aacccgttcc tttcccgaag ttcgtgcttt cacctgaaag acgttccgga gaccgaggtc
     2701 gacgattctg ctggtatcgc cacccaggct acgtccgccg aagacgtacg tgccgccccg
     2761 cgtacgggaa gagaacacgt aattgaacga cgccgagggg gttacgacgt ggcggaccgc
     2821 gctgaccggg cccaaatgaa ccggaaacac accgtagaat gtggcgttgc tggagacgcc
     2881 gccggagatt gtctgttgcg tctgcgggtc gttggcgttc agttcgtcgg ggcggctttt
     2941 ccacgacgca gaccagttga acgtcggcac gagggtcagc caccgtggcg tggggagcgg
     3001 caacgtcaac ccgacattct gggaagtggt ctgcgtgtag ccatccagga tcctcttgcg
     3061 cgtccagagg acttcctgtg cgatatcagg ctccttcacg cggagggagt tgccggacat
     3121 gatcgcgtag ccgtcgggaa ggccggcaga gcgggaaatc agcacgaggg cattgctgtc
     3181 gctcgtccgc tgaacatagc cttcgccgat tcgttccccg ttttcgactg caacgacata
     3241 ggtgccgtca tagagcgagt tgatgtagct cgtgctggtg atggtgagga gatagacgat
     3301 cacgctgtct ccgcgctgtt ctgtcgtctc ccacgtgtcg aaaatggttc tgtcggacgg
     3361 acttctccgc tggctgttcg ttccactcca cgaccaggag tatccgagcc tgcggaacca
     3421 tgccggctcc tgacgagggg ctgccgccgc acgaggcccg cgttggggag gcggttcggg
     3481 gaagaagtag cgttggccgg aacttatgga gagggccgga agcatcacgt ccaactggcg
     3541 attctgaagg ttctggctcc tccgcgcgtc aacgcgcagg ctgtacccgg attcccatgt
     3601 ctgagtgtat ccgaccgtcg aggtgaaatc ctgcctgagc acggacgcct gggttgaact
     3661 ggaggtgacg tagggcgagg aggtgctggt gaagttgatg ttggcagcca cggaacttcg
     3721 cggagtgagt tcctgccggt gttgcgcggt tacagtccag atgcgccgcc cgatctggga
     3781 gggtgtatcc cagtacttgc tgtaatccga cgcgatggag ccctggaagc gataacggag
     3841 cgcgtaatcc gtccgcagcc gccattcgta cccaatgcgt tcgtctatgt cgagcacggc
     3901 ctgtcccccc acgtagtcgt tgggagcgac gtagtaaccg agcccacgca tgaatcggcc
     3961 cgcgtatctg ttttcgccca tgcggggcgc gatgaatccg gaacggcggt ccttgtcgac
     4021 aaaaaacaca ccgaacggga gccagaagaa ggggacgtct gccaccttga acacgattgg
     4081 tttcgcgatg accttgtcac cgacgagtac gcgcatctgc ctggcttcaa actggtagtg
     4141 cggcgggttg tcgtcgcacg tggtgtacac gccgttcaga acatctacct gcctttcacc
     4201 ggtccgcttg acaacgctcc ctccgtagat gccttccgtc acttcgctgt ggccctcttc
     4261 caccaagcct ctgcgcgtgc gcaggttgta cgtcatccgt gcacctgaaa aggcttcctg
     4321 cccgctggaa aactccggcc tcccgataat ggagtctggc gagattgcgt ggccttccgc
     4381 gatcagaagg tcttcgcgtg tgaggaaccg aatacggtgc gccgtaaggc tcatcccggc
     4441 cgcggacacg cgtgcgttgc caaggagagt gatcaccctg gtgtcgaggt catactcgat
     4501 ttcgtcggcc gcgtaccgaa cggaatcggt ggacacgctg tcagggaaac cgccgaacgt
     4561 ggcgaagcgc tcgccggtag cggtagaagg aagctgggcg cggaggggag caggtaacca
     4621 caggatgagc agcacggcga tctcacacaa tccgcacgcg attctcaagg cgcaggggac
     4681 gcggagcgcg tgttcgccgg gctgcatcgc aatctcagga agaagccgcg gattcgccca
     4741 ggacggagac tctgacctgg cggttcctcg ggccgtcgaa ctcacaaagg aatatcccct
     4801 gccacgtacc cagctggatg tttcccgttt ccacggcaac ggttacggat gatccggtga
     4861 gaagcgcctt caggtgcgcc gggctgttcc cttccgaatg ggcgtagtcc ccgtcccgcg
     4921 gaacaatgcg ttcgagcgcg acgaggatat cgcgcggaac atccggatct gcgttctcgt
     4981 tgatggtaac cgccgcggtt gtgtggggaa caaagaccgt gcagacccca ctcttgattc
     5041 cgtgttggcg aacgatgtcg aagaccgcac cggtgatgtc gatcatctcg tttttccggc
     5101 gcgtcggcac ggtgaatgtt gtggaagaga acatggaaac gacccctacc gcaaacgcag
     5161 tgcgagaacc ggggaaaaga actcggcaag aagaacgaga acccacgttc tgccccgaaa
     5221 cacgttgtag tcagccagaa gcttttccca cgggtggttc atcacatagt gtccgaacac
     5281 gaattcgaac agcaccgtgc atatgaccca tcctgcgccc accaggaacg cctctctggg
     5341 tccgggaatc gcattccgca tgaaccacca cgtcagaagg aacacgatgg cgctgagagt
     5401 aactgaagac acgatatgtc ccgcgtgctc accgataacc gggttgatcc agtagacgcg
     5461 gacagtcccg ttgagaatag ccagcggaat cattgcaatc cagaggagaa tcgcccgcac
     5521 gacaagcatc acgcacctca tgttcgggaa gctgttgcgt tcacctggtt ccaacaacaa
     5581 gcccggtagc accgggaagc ggcaccgaac gaattctgct gaaacctgtt tccttcagcc
     5641 acgcgtggta ctcctcctcg ctgtaggaat tcccggctgc cgtgttaaca agcatgttca
     5701 gcgcgaacag aacgcctgcc ttcggcgccg tcttgtccgc gttgaggatg tgatcatgaa
     5761 ttaccagatc accacccggt tgcaaagcgg cgaaagcctt ccgaaacagc gatctgtttt
     5821 cctccgggcc attcatgtgg cagatcgcgg aaaggaacac aagatcgaag ccgtttccaa
     5881 acggatcgct gtggaaatcg cctcttttga cgctcacgcg gtccgccact cccgcgtcct
     5941 tgatgtgccg tcgcgcaatt cgcgtaaccg ggcccaggtc aaacaccact gacctgaggg
     6001 ccgggttcat ccgggcgaag gtgatgctat accccccgga accgcccccg acgtcgagca
     6061 tattttcata ccttgcaata ccaagcgcac ccgcgaccac agacgcccgc tcgagagagt
     6121 ttcggtgcat cgccgcgatg aaggcttccg tctcttccgc gttgcgtctg cgtttcgcta
     6181 ccgaggtccc ggcgcgcacc gctgccgtta acgtgctcca cgaattccac aggcctaccg
     6241 tgtgcatgat agcggcgcgc gcgctatcgg gagaagttga ggtaaggtac ttttcggcaa
     6301 cgggcgtcag cgcgtaacga tcttcggctt ttttgaggag gccctgggtg accagcacgt
     6361 tcatcagcat ctctgttgcg cgcgggtcgg tcccgatttt gtgcgcgacc gaggatgctg
     6421 atgatccctc gcatactgcc gcgaagaggt ccagttccac cgcggtcagg attgcgcgac
     6481 tctccatgaa cgcccgcgca gtggccatgg tggcgtcgaa cccggttttc agcgatgtga
     6541 tgttcgtatc gtccatcggt gtcgatcctc tatccgcgga tgcggaccat gaccttgtac
     6601 atccccacaa agcgagggaa ccactcccaa aaaagagaag ccggagggcc gtgctgtttc
     6661 agaagcgacc ctccggcctc gtactcgaat gtaacgaagc tacatgagct gcgcaccctc
     6721 ctcgacaacc agttccgggt gcttccgggc tgtccggcga tcgcgccatg ccctgaaacg
     6781 gatggccatg gtttcgaaca gggtgtacac cacgggcacc actatcaggg tcataagcgt
     6841 ggcgaacaac aggccaccca tgacggcgcg cgccagcgga gcccaggatt cacctgattc
     6901 acccagccca agtgaaagcg ggaacatgcc catggtcgtg gtgagagccg tcatcaacac
     6961 cggcctcagg cgggttcttc cggcttcata cgcggcttca aacagctcct tgtgttcgag
     7021 ccgcatcatc tggttcgtga aatccaccag aacgattccg ttgttcacga cgatccccac
     7081 gagcatcaac atgccgagaa gtgccatcac gctcacggtg gtgccggtta ttgccagcgc
     7141 gattgcaacc ccgataaacg aaagtggaac cgtggttccg atgataagtg gatcgacgag
     7201 cgattcgaac tggctcgcca tgaccatgta cacaagcaca attcccgcga ggatggccag
     7261 cccgaggtac cggaacgatt cccggagatc ctccgctccc ccaccgactt ccatgatgac
     7321 gtcgttggga atgttgagtt tgctgatcgc cttttccaca tctttcgtca cgctcccgag
     7381 gtcgcgtcct gatacgccga tggaaacggt tgccacgcgc ccctgattct cacggaaaat
     7441 ggcgatgggg gccacttcac gtctgacatc tgcgacctgt cgcaatggga tcagggcgcc
     7501 cgcaggtgtg gcgatgatga ggttctccaa ttgctccgga gatcgccgga attcctcggc
     7561 aaagcggaca aaaatgttgt actcttcccc cgcttcacgg taccgcgtgg ccgtggcgcc
     7621 ctgaatggcc gtggataccg ccgcactcac ctgcccggcc gtcaggccga ccgcctgaag
     7681 acggtcacgg tcgagacgta tctgaagttc cggcgcccct cgatcgaggt tcgatctggc
     7741 gtcgactgcc ccggggattt ttttggcgat tgccacggtg ctatccgcga gattcccgat
     7801 cacgtcaaga tcgtctccga agaaacggac ctcgatgtcg gcgccgccca tgcccggacc
     7861 gctatcctgc tggaagttgt acttgattcc cgggatcttg tcgaagcgag cacgaagagc
     7921 gtcctgaatt tcggacttcg agcggtcccg atcctggaga ggaacaagtt tgacctgtat
     7981 ttcgccagaa tgggtgtcga gcccctgcag ggccgcgacg attccgccgc tggctcccgc
     8041 attcacgtat accgtcgtgc gctccggaac ctcctttttg atgatctcct ccatcatgtt
     8101 gagcgtcgcc atggtggttg gcaaatcggt gcccacggcg tgctgcacac tgcaacggat
     8161 gcgaccgtcg tctggatgtt ccatgaaatt gacgccgatg ctcatgaaca gaggaatcga
     8221 cgccgcgaac aatcccgctg cggtgaagac aaccgtcttg cgatggccaa gcgcccattt
     8281 caacacaacg ctgtagcggg tttccagcca tttcagcccc ttgccgatgt cttcgtacat
     8341 gcggagcagg aatcgtgtcc ggggcgggtg ctgccgggcg aggaaccccg cagcgaggga
     8401 gggcacagcg gtaagtgcga cgaagatgga cacggaaagt gcgaagcaga tcgtcagcga
     8461 catgtcgtta aacatgaccc ccgcaattcc cggaacgaac aatacgggaa gaaacacaac
     8521 gatcgtagtg agtgtcgagg cggtgatagc catcgccacc tggttgcttc ccttttccgc
     8581 cgcctccacg cggtcgtacc cgagttcgtg caggcggaag atgttttcga gcacgacgat
     8641 gctgttatcg acaagcattc ccaccgcaag cgcaagcccg gccatgctga taacgttgaa
     8701 cgtgactcct gctgcatcca tgacggagaa cgtgagcacc atggaaaccg ggatcgagat
     8761 acctacgatc agagagctcc ggatattccg caggaagacc agcaatacga ggattgtcaa
     8821 cccgaatgcc tgcagggcgt tggaggagag attccccagg gaatcctgaa tgtactgggc
     8881 ctggtcaaaa acagtcgtca acgacagatt gcccgggacg ctctgcgtga tctccggcag
     8941 gactgctttc acggcacgca cggtttgcac ggtgtttgcg tccgattgct tgcggacgat
     9001 cagcatcaca ccggactctc cgtcaatcct tgcctcgccc agaacctcct ggaagccgtc
     9061 gctaacatcc gccacatcac gaaggcggat cacctgtcca tttttcgtgg ccagcatgac
     9121 gtcgcgaatc tgctcgaccg aaaggaactc gccaagggtg cggacggcga atgcggtgcg
     9181 cccatcgcgg attgaaccgc ccggcacctg catgttgctt ctctgaagcg cggccgtgac
     9241 ggtctcgatg ccgaaaccgt acgatgcgag ggcgcgcggg tcgatctcca cattgatctg
     9301 tcgcgcgagg ccaccgacca cgtccaccga ggcaactccc tccacccgtt tcatcctcgg
     9361 caccacgtag tcttcacaga ccttgcgaag gcttgcctga ttgagcactg gtgaacgcac
     9421 gcccatgaac atgatgggca tcattttcgt actgaatttg aacagccgtg gcttggtcac
     9481 gtcctccggg agagtggtaa cccggtccag cgcacggctt acgttcatct gcgccatgtc
     9541 catgtccgta tcccagtcga actccagcga gattacggac attccgttgc gcgaacttga
     9601 ggtgaccttc ttgattcctt ccaccgacga cactgccgat tcaatgggct cagtgacgac
     9661 ggactccatg tcaaacggcc ctgcgcctct gtactgtgtc aggaccatga tcatcgggaa
     9721 cgagatatca gggtaaaggt ccagtttcaa ccttgagagc gaaaaaagcc cgaacccgag
     9781 agctaacaga tacgccataa aggtggtgac atgatggcgt atggcgagac gtggcagatt
     9841 catgaagaga ctcccgcggt tctattcaga tgccggtgaa acacgctgcc cgttctgcag
     9901 gaacgagtga ccttgaacga tgattttgtc gtccgggcgg attcctgagg tgatctccac
     9961 acggtcccct gttgtgtatc ccagcgtgac attcctccgc acggcccttt ctccttctgc
    10021 cacgaagagg tagtacgttc tggttatatt gctgacggta ctccctgcca caaggctggc
    10081 ctcctgcaac acggcgttgg aaggaaccat caacgcattc gggcggcgat cagcttcgat
    10141 ttccacacgg gcgtacatcc cggctttcag gcgctgatca cggttgggca gcaggatttc
    10201 cacatcggcc atgcgtgtca tcgcatccag aatcgggctt atctgtctga ttgttccgct
    10261 gaacgcttca ttcgggtacg aagccacctg aacagttgcg ctctgaccga ttctcagggt
    10321 cccgaaatcc ctttccggaa cctggatctt ggcaatcaat ggcgccggtt ccgttactac
    10381 cgccagagga acaccggggc caaccatgtc gcctacctca acaaatacct ttccgacgag
    10441 gccggaaaac ggcgcgcgca cttccgcatc gtttctccga atgaccgcct gctgcagcat
    10501 ggcgcgtgcc tgtttgacgc cttcgtccgc cgcggccttt tgcgtgcgga tggcgtcgta
    10561 ctgcgactga cttgcgccgc cttcggcgta gagacgctgt acccgcgcaa attcggtggc
    10621 aaggttggca gcctgctgct cggcagagga aacggctgcc tgcgcctgct gaaccgccgc
    10681 gtccagcgag gagtagtcca ctacgaagag aacctggttt gccgcgacac gggaaccgag
    10741 atccactcgt ttgctgacaa tgcgtccgga aaccggcgag atgattgtca cctgtttgtc
    10801 tgccaccagg cttccgttgg cggaaatgac gcgaatggcg tcctctcggt acggagcgtg
    10861 gacggtgact ggcagcgcct gttcggctgt atctgcctgt tgttcttttt caccacagcc
    10921 cgaagaaccg atcgcgacta tcgcaaggaa cgcggtcagc gcgagtgcag atgtccagcg
    10981 tcggtcgccg gtatgcgtcg agatgtgatt tcgaatcctt ccttccacgg tgtgctcctt
    11041 cagtcctgac tgacccgggt gagaccgagt gccttttcca tgcggatatg ggcaacgtga
    11101 tagttgtaaa gagcctgtgc gtactgggtg cgcgcgctcg tcagcgcaag ttgcgcggaa
    11161 atgacatcca gttgcgtatt tccgccttcg ctgtacatca cactggcgag gcgcaacgcc
    11221 tcggcggcct ggttcacggt ttcgaggccg gtggcgaggt tcagctctgc ctcatgaagc
    11281 ccgtggaacg ctgcctccac ttccagcgaa atcccgtctg tggccaactg ccggaacaga
    11341 ttgagctgcc ggacgcctac ccttgccatc tgcacggttg ccggtgtttt ccacgaatcc
    11401 cagaacgtcc agttcaactg caatcccagc atggagacgc ggcggaaatc accggaactc
    11461 acactcagtg atggccgttg tgcctcccac tgagaggtcg cgaacgcggc gacagtcggg
    11521 tagtacgcgc tgcgggaaag tgttacggcg tcattggaaa tctgttcgcc aatatcgagc
    11581 tgccggattt ccgtcctctg ttcccgcgcg atgcgtcgaa gggaatccag aggcaccttc
    11641 gcccattcgc tcgcgaaaac ggccagcgtg tccgaaggtg tgatcgcgtc tcgttctccc
    11701 acgcctatcg cgaggcgaag cctgccatcg gcaaggtcgc ggttgcttct cgcggatgtc
    11761 agattcggcc gcagagtggc caactggacg cgtgcgcgca ggagatcgaa gcgcgatgcg
    11821 gagcccacgt ccgccctgcg ttgaacaagc gcaaggctgg attccgcctg cgccaccgcc
    11881 tgctcgttca ccgtgacaag catgcggctc aacaccgccg cgaggtacgc ctccctgacg
    11941 gatgcgatca cggtttgagt ggttgcgtca aggcggagtt gtgaggattg cagcgctgtt
    12001 tccgccatcc gcacaccggt gactccggcg aaaccgagga aaacgggctg gcgggcagtg
    12061 agagcgagcg agtagttgtg tttttcaccg atcgaaaccg ccccgaccgg cgccggcatc
    12121 acaaccaccg gcaattccgc gttccaggtg tatcccgcgg tggccgcgag gctcggcagg
    12181 atcgtcgcgc gtgccctggt tatgcctgca cgggcgctgt cgaggctcgc tttcgcgccg
    12241 gcgatctgcg gattgcgctc caacgccatt tgttccgcct cgcgcaacga aattcccctg
    12301 gcgccggctc ctgacaccaa agccatggaa cacacgatcc agaaggcaca cactttgcca
    12361 gtgcggccga tgctctccaa catgcaaagt ctccttttca cgcgcttatg aggcaggtaa
    12421 ttccgaaggg gaaaacgaag acttcctcac gcgcacactc cgtggaggta gagctgagtt
    12481 aactggtcgg ccagatcgtc gtccagcgga gggccgccga gaaggatgct ctgtccgacg
    12541 tgaatattga ctgctcccga gaagacgttg atcgcaacgc tggggttaat gtccggacgg
    12601 aacaacccgc tcgcctgtcc ttcggcgaac atccgtaaga gaatgtttac tattggcttg
    12661 tgttcacggc ggaattcggc cgccaacccc gcttcatcag gcccgagcag gaaattgata
    12721 tgaaaacgca cgaggtcggg gttttcccgg acgtgtcgga actgcgctct gactaccctc
    12781 tggaaacgct cgatgagcgg gatgtccagg cgcgcgattt catcccacgt ctgactcagg
    12841 ttctggaagc cttcttcaag gagcgctctg cacagaccag ccttgttttc gaaatagtag
    12901 taaagcatgg gcttgctgac gcccgcggca tcagcaattt cgcgcatgga gacgctcccg
    12961 tacccgcgcg ccgcgaatag ctttgccgca gccgcaaaga tcctgtcgcg ggtttccttt
    13021 ccgacggccg ggtgggatgt gggttccatg aggcggttga tctccgtttc tgtgtctacc
    13081 taccggtaag gataataaca accaaccacc tctccgtcaa gatgagaacc gcgtccaggg
    13141 tacaacaggt aagacttcca accgggacag aatgttacat tttgagggcg cggaatgaag
    13201 gcgaatcagg cgagagtgac gggtccggtt gcgatggcga acacgcagaa agaggcagca
    13261 gcgcgggcgg aggatctccg acagaaggtt tcccttctgc cgagaaagcc cggcgtgtac
    13321 atttttcgcg attcaagcgg ggagataatc tacgtaggca aggcgaaggt ccttgcaaac
    13381 cgggttcgca catatttcgc ggacagctat gatgacggtc gcctccagat tcacgcgctt
    13441 gtgcggagca tcgctgacct ggaagtgatc gccaccagca gcgaaaccga agctctggtt
    13501 ctggaagcta ctctggtcaa gaagcatgct ccgcggtaca acatccgtct gaaagacgac
    13561 aaaaaatacc cgtacttgtt gctcaccaac gaggcgtttc cccgcctggt tttcacacgg
    13621 tcggttaggc gtggtgaggg caggtatttc ggtccctaca cagatacccg cgcgctccgc
    13681 cgcactcagg aactgctgca ccgtcttttt cccctgcgca cgtgcacgtc tcccctgccg
    13741 agtccgtcaa taacccgcgt gtgcctgcaa tacgaaatca agcggtgcaa aggcccgtgc
    13801 gtgggactgc agacccagga agagtacgcg acggtgacga accaggcgga gcgattcctg
    13861 agagggcaga tttcgagtgt cgtgagggaa ctggaacagg agatggacct cgcttcggaa
    13921 aggctccagt tcgagcgcgc cgccgaactg cgggaccggt tgaatgacat acggacaacc
    13981 gcggagaaac agagcctggc agatgcccgc ggggaggaca gggatgttgt cgcgcttacg
    14041 cgcgacgaca cggaagcagt cgcggttgtt cttgaaatcc gggaaggcaa actgattgcc
    14101 cggaaggact attccctgtc ggtcggtctg gatgagccgg tcgaggcgat catgggggca
    14161 ttcttgcgcc agtactacct cgacgcgatg gcgataccgc gggaactcgc ggtgatgtgt
    14221 gagccggagg atgcgcatgc ccttgaagaa ctgctgggcc atcagcgggg agggattgtc
    14281 cgcctgcaaa caccgcggcg cggggaaaag acccggcttg tcgacatggc tcgacagaac
    14341 gcggagatgc tccttgctga gcgccgcctg cgccgggaga aattccgggg ccgggtgccg
    14401 cactcggtgc gagcgctgga gcgcgatctc cggcttcccc gtccgcctcg caggatcgcg
    14461 tgtttcgata tttcgcatat ccagggcacg gacacggtcg gctctcttgt ggtttttcag
    14521 gacggacggc ctctgaaaaa cgcgtatcgg atgttccaga ttcggggcgt cagcggaagt
    14581 gtcggggaat ccgacgactt cgccgcgatc cgggaagtag ttgaacgggc attctcccgt
    14641 ctggcgaaag acacggagga agcaagaccg gacctcgtga tcgtggatgg gggaaaaggt
    14701 cagctctcaa gtgccaaggc ggtgctggat tcactgggtt tcggtgacca gccggtaatt
    14761 ggtctcgcga agcggctgga agaggttttt ctgccgggtt gcagtgaacc tcagaccatc
    14821 ccccggacgt ccagcggatt gaaacttctg cagcaggttc gggatgaggc gcaccgcttc
    14881 gcaatcgggt accaccgaaa aaagcgcggc agggcaatgt ccgtaagcgc gctggacgag
    14941 gtgccgggga tcggaattgc cagaaagcgt gcgctgttga gaaaattcag atcgctggcg
    15001 agtcttctgg ccgcgccaga agaggaaatt gcggcggtgg aaggcatcgg cccggttctg
    15061 gcggctacga tccgggcggt gctgttggac ggcagaacgg gcggcaggaa cggcgcgcag
    15121 agtccgccgc tcaaggtgca gacgagttga cccggggctt cgcgcaggcg ttaattcaga
    15181 tagccggatg gcgcgcaatc agacgccgtt tcgcagaaac tacgagaagc attgcccgga
    15241 cgcatgggcg gtgctccgat tttcagaagt gacgtacggt ggcagggaac gtcgaatcta
    15301 ctcgagttgc agccgaacgc gcggcgaagc gccttcggcc gtatgctttc cttcaggcag
    15361 gcgacactct gcgcaaatgt ctggacggat tcccgagtgg cgccgtggca cctttgatca
    15421 tcacaactgc tgcctgtgag gggattccgg aggatttcag ggtttttgtt ccggcagatg
    15481 cgatagaagc ggaaaaagca gtcatctcat cggggcttcg gcctgcggtg attgcagtga
    15541 ccgactcacc agttcccgcg tgcaccgcac tctcagacgt tctcgaaatc gggatgccaa
    15601 acctgtaccg cgaagggcag gacggcgcga tagtgacctc cgggccggct cttcatgtgg
    15661 cgctggaggt tgccgatgag gctacccgaa acgggttgtc gctgacagtg atccacctgc
    15721 ccacgctttc tcctctgtgc cgcgcggagt tgtaccgcct gctgccgtct cacgtacccg
    15781 ttgtgtttgt ttcaccgcat tctctcgcgc acacggtcct ggaaagcctc cagagtgagg
    15841 ttccgggatc gcgaatcgct gaagcgacgg cacctgcgtt aaccagagcg ctcgcagacg
    15901 ctttgcctga aggagccgga tctgtgggca aaacgcgatt cgcggaacgg atgaaccgtc
    15961 tcggcaccga gaacgcgttc gaggttctcg cggttatcaa ccggctgagg gctgaggggc
    16021 gggatattgt aagtttcgcg atcggcgaac ccgatttccc gtctccttcc aatgtcaaac
    16081 gagccggctg cagtgccatc atggaagaca agacgcatta cggggagtcc gcgggattgc
    16141 gggagctccg ggaggcaatc gccgcccata tccggaagac gcgcggcgtg gatgccgggc
    16201 cggaaaacgt ggtggtgaca ccgggggcaa agcccattct tttcaatacg atcatgaccc
    16261 tcgtagaccc cggcgacgag gtgatgtacc cgaaccccgg gtttcccatc tacgaatcgc
    16321 tcatcgattt tgtcggagca aagggcgttc ccgtgcccct ctgggagtcg ctggacttca
    16381 acttcgatcc tgaggagttt cgccgtctcg tcacaaaaaa gacgcgcctg gtgatactca
    16441 acacgccaca gaatcccacg gggggaattc ttacgaggga cgcgttgcag gttgtcgcgg
    16501 agatggctca ggaacgcgat ttctgggtcc tttcggatga agtgtattcg cagatggcgt
    16561 acgacgggga atttctgagc attgcatccc tgcccggcat ggcggaacgc actgtggttc
    16621 tcgacgggtt ctccaagacg tattcaatga ccggctggcg cctcggatac ggggtaatgc
    16681 cggcggacgt tgcccggcag gaggctcgca tcgagacgaa cctcaactcg tgtacggcca
    16741 cgttcaccca gtgggcaggg ctggaggcgt tgacgggtcc gcaggacgaa tcgctcgcca
    16801 tgatcgccga gttcaagcgg agacgctcgc tgattgtgga cggtttgaac cgtatccccg
    16861 ggttttcgtg tcgcaaacca gcgggtgcat tctacgcgtt tcccaacgtc accgaagcgt
    16921 gccgcatgct caacaagagc gattcgaagg cgctgcagga gtatctgctg ttcgaggcgg
    16981 gcgttgcggt gctgccccgg acgtcgtttg gaaggcggaa cgccggggag aaggacgaat
    17041 atgttcggtt cagttacgcc acgtccgctc cgctgatcga ggaaggattg gagcggatca
    17101 ggaaggcgat ggagggttaa gtagaaccgg ttgaggactt ggtgtgtgcc agatcagttg
    17161 gcgaataatg cggttttcgg tgtaatcacg tccgtttcag ttccatgcgt tccatacccg
    17221 gggagggcag aaggatgcca cgaccagtaa cactgtttac aggacaatgg gccgatctca
    17281 agctggcgga tcttgcgccg aaggcgaagg ggttcggata cgacggtctt gagcttgcgt
    17341 gctggggcga ccacttcaac gtgaaagaag ccgccggaag catgcagtac tgcgacgaaa
    17401 agaagaagct cctggcgaaa catgggctga aagtctggtc gatttccaat caccttgccg
    17461 gccaactcgt ctgcgatccg aacgacgagc gcgcgaatgg ctttgcgccg ccggaactgg
    17521 cgaacaagcc gaagaagatt cgcgactggg caatcgatga gatgaagctg acgggagttg
    17581 cggcgaagaa cctcggtgtg tcggttgtca acggtttcac cggctcggga atctggcatg
    17641 cgttctacag cttcccgccg tggactccgg ggatggttga ggcagggtac aaagattttg
    17701 cgcggaaatg gaaaccgata ctggacagct tcgccgacaa cggaatcaag ttcggtcttg
    17761 aagttcaccc gacagaaatc gcgtatgata tctacaccgc gcaggccgct ctcaaagcgg
    17821 tgaagaatca cccggcgttc gggttcaact ttgacccgag ccacctgcac tggcagggcg
    17881 tgagcccctc ggagttcatc cacacgtttg gcgacaggat tttccacgtg cacatgaagg
    17941 atgccatcgt caaacttgac gggaaaggga gcatcctcgg ctcgcatctc aatttcgggg
    18001 attaccgccg ggcctgggat ttccggagcg tgggccgggg cacggtgaac ttcgaagaga
    18061 tcattcgtgc gttgaacgac atcggctacc aggggccgct ttcggttgaa tgggaagaca
    18121 gcggcatgga ccgtgaacac ggcgccaagg aatcctgtga gttcgtgaaa aagctggatt
    18181 tcgccccttc cagcgtcgca tttgacgcag cgtttgagga aaaaggcaag acggccaagg
    18241 catgatcgga gaaacgtccc cctcttccgc ccttgcgcgg aggaggggga cacgcatgta
    18301 cagttgacga tttcctcatt atcccgtact ttttagaatt cctgtgtatc ccccgcggca
    18361 cgagacaccc cacctgtttg cgtgatgccc gggcctttgg aatgatgtat gcttccctgt
    18421 acggaagact acttcgggcg tttccgacga cacgaacgtc cgctacttct gccggaagct
    18481 cccggttgag cttcttcaca ttccgtctca tgtcctggaa aggattatcc atgaccaaac
    18541 gtttcctggt gcatccgttt gtgatgctcg ggctcggggc attgcttttc gtgggcgccg
    18601 gttgcgaatc cgtgggtctt cgttcgatga agatctacat tcagcagcgc aactgggaaa
    18661 aggcgctcga acagggcaac atcgcgctgg cagagaaccc gaacgatgca gaagcatggt
    18721 ttgccatggc gcaggtggct gcgcaggtcg attcatttga cctcatgctg agggcgatcg
    18781 agcggaccga atcgcttacc aacaagcatg ccgcggattt caaactcatg cgcgaggcga
    18841 aatacgggtc ggcgttcaac atgggagtcg agcgctacaa cgccaatgac atggcggggg
    18901 cctcccggtt catggatatc gctatctcga tcgacagcac acgggcaaat gcgtaccgcc
    18961 tgaagggaat gatcgcccag cgggccggga acacccagga aacgctccag tatttcgatc
    19021 gtgcgtaccg ggccgacacc actgacgtcg aactggcacg cgtgtacgct cagttgctgg
    19081 acgccatggg aaagagaaac gaggctttgt cggttgcgca ccggctctac accgcgcggc
    19141 cgggcgacaa gaaaacggtg gccctgtacc tccagcttct cactgaagcc ggccgttttg
    19201 aagaggctct taacgtggtc aacaccgccc tggccagcga tccgaacaac gccgaataca
    19261 acatgcgggc aggcgttctg tggatgcagc gcgcgcagca ggatgttgct gactccaccg
    19321 ccatcgtgcg tgacctggaa acggcagccc cgtatttcga ggcagcggtg gaagcagaca
    19381 cggcgaacac tgacgcggcg tacaacctcg cggtgtgcta tgcccgcctg aaccgccgcg
    19441 gtgatgcgat caacgtcatg aagcggttgc tcagcgtcag accgaacgat taccaggcgc
    19501 gcatacaact gggaacgttg tacgtccgtg aggagaaccc ggatgccgcg gagcaggagt
    19561 ttctggcggt cgtggagtcg atcggcgaac ccacgaatgc agaagatcgt gccgttgcat
    19621 ctcgcgcgta ccgctacctc agcaccatct acacctaccg cggcgcggtg aaacagggtg
    19681 aagcagaccg cttgcgggaa cgggcacagg agctcatcaa cgagtctgca atggcgcgtc
    19741 agcggactgt tcgtcagcaa aaagaacgag aggctcaggc tcttttgcag caggcggaac
    19801 cgcttgccac tgaagcgcag gcactgaacc agcgcagcgt cgaatatgac cgtttgagca
    19861 accagtacgc aaactgagct gcgaaccaac gaaaaagagg gagggttccc ggcgcgggtc
    19921 ccctccctct ggtttttccc cgtttctact tggttccgta caccgcctgc gcctggaaga
    19981 cgcgatcgcc gtcctcaacg agcccatcgg gagtcagttt ccggtagaaa cagctgcgga
    20041 agccggtgtg gcaggcgccg cctttctgac ggactttgaa caggagcgcg tcgccgtcgc
    20101 agtcgatcct catttcgacg acctgttgtg tgtggccgga ggtctccccc ttcacccaga
    20161 gttttcctcg gcttctgctc cagtacgtca tgatgcccgt gcgcagagtc gcttccagcg
    20221 actcgcggtt catgtatgcg agcatcagga tttctccgtt gtcggcgtcc tgagcgatag
    20281 ccgtgacgag cccccggtcg tcgaatttca cctggtcaag catcgttgtt tcccgaatca
    20341 gtagaagagc gccgcggaga ggagtgccca cagaatactt gccgcggcga gcggtttgtc
    20401 gttgagaatc agaacttcgg gcgcgttcgt cgagccggcg acgtacagga ggtagagata
    20461 gcggaacacc ccgtacaaca cgaagggaat agtaagcatg agcttctcgg aatgtcccga
    20521 atgaaacgtg aacaggcagt acgtgatgag ggtcattccg gcaacaatga caatcaggac
    20581 atcaaggaat ggtgccgggt actcgccaag aatgcgccgg tgtttcgccg cgttctctcc
    20641 gagaagcagc gcttctcccc ggcgctttcc gaggctgatg agcaatgcga ggcagagcgt
    20701 gcagagcaga atccacggtc ttacggggac
//
LOCUS       DAOTNG010000013        19711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_2821_length_19711_cov_4.815883, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000013 DAOTNG010000000
VERSION     DAOTNG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19711
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_19711_cov_4.815883"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="P5179_01350"
     CDS             <1..423
                     /locus_tag="P5179_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HRS93900.1"
                     /translation="PAFAMVDTLSLPVVGAIAAGSPREAIQQTDRWVERLEDLLPVRP
                     GDFLLTVNGESMIGDGILPGDLVLIRPQEVVEPGQIAAVMVRNDEATLKRFYPQPPSH
                     VRLVPSNPTMDDMVYPAEEVRVIGKYQGLIRTGHHGRQ"
     gene            449..832
                     /locus_tag="P5179_01355"
     CDS             449..832
                     /locus_tag="P5179_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93901.1"
                     /translation="MPVTLHSTDERTEVQVRVDDSAMVDPLQLRRDVIAALQRSARKE
                     ITLSLEGVAKIDTRLLSAIKMAVTEARIRRRRIILVSAPAAIRFALSGTSFRVRWAAG
                     LFPLDTESPAGALILNRYTSRVSAV"
     gene            857..1852
                     /locus_tag="P5179_01360"
     CDS             857..1852
                     /locus_tag="P5179_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93902.1"
                     /translation="MDAQSLAKTVQTPFKHGSLILSPSFRPGDFDSHGVDCPFVFRHE
                     DRFLMTYIGFDGLGYRTGLAWSDDLLVWHREGMILDRGIAGSVTQHNAALTWILRDND
                     LFGTGCLRQIDGRYVGTYHAYPAQGYESGPAVIGICSSTNLRDWEIGEPCLIPAEGEE
                     WERGGLYKSCLIEFQRRYYLFYNAKTVGVPWIEQTGVAISRDLMRWERYGGNPILRTG
                     AEGAFDERFASDPCVLRVGDAWAMFYFGLGRDGHARDGVAFSRDLLHWEKSGSVLVDV
                     GPPGAVDSRYAHKPSMFWHRNRLFHFYCAVSPRNEQAAVGAVHTSETRGISVAFS"
     gene            2045..4369
                     /locus_tag="P5179_01365"
     CDS             2045..4369
                     /locus_tag="P5179_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRS93903.1"
                     /translation="MPRRPALYPRQKWEWAKDLPVSDAPWEDLLRLPDIRFLTDNQVS
                     LWTSPDSIGLEKVWFYPVPRAPHLEVERFDSGRIAQSSVHVLRGPRGALMAGGKPVTW
                     DTQNLQWSPAEITRTFVSPEVTLQETVTIVEDAIVVWLRPVEGTSELPLSAAWSSSVE
                     RTLSGSWRMHDGARVCMHDDGIAHGWVWFGSEVTNWQGKAASGKFGWEASIPPQGGVL
                     VLLLGYDQAAVERRLEAMIAQCSNRPTADIAELFLTKARDAWQWYMKRMVPGLKGAEE
                     WVRRLYYYQTALHKINLYDIPYEPFVYPYTCPWKTGAVWQWSWNTPMNSIAERWLNDP
                     AWAESGIELLRENGGALNIGASLHRLRKPRQFRDVNEYLPALQDAIGGVKNLPATARQ
                     FDWAFIMPHTTPLGVHGIWEVFRRTGDEDYLRRQLPEMVAYEARLTSHDADGDGLVEY
                     EGMVDEYDYSLRWRTAVNGYKKGTESLIRFDRPLELIDINAQLCLLREDIIEAAGIVG
                     DRNLAKRIRQRLNKTARAINDLMWDETRGWYGDLDAVTHELTGVCSVAAFSALMSGVA
                     PADRAERMVRLLQDPAAFGTPYPVPSVAADTPDLDPGHITYGGDVLITSGVWLTVVAL
                     VRYGFLEEARRIIWKALRMLGENAPTSSYSYNSMTGAPNMDPHTFCSQSAITLDLFVR
                     FVVGFVPRYDDIIECWPFALPTEWEHLEFGPMTWRGDVDVTVVWDSESGYTVRAGRSE
                     FTAREPRHLWLALDQRDRLVELPDPSVSGMPVMT"
     gene            complement(4359..5567)
                     /locus_tag="P5179_01370"
     CDS             complement(4359..5567)
                     /locus_tag="P5179_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93904.1"
                     /translation="MGAKVQLDPSPFGMGIYPCHRTSGETTLRALGLARDAGIKWTRD
                     ELGWGGIQPERGVWKWEAFDRAIRLTREHGIHTLGLLCYSAPWAATHRTAEGKPSVVS
                     LPDLSAWKEYVFRTVERYRETIHVWQMWNEPNISGFWQPKPNPREYAKLLVAGAEAAR
                     AADPTCWIVGCNTSMVDLAFDRVVFEEGGWDTTDIIGVHPYRYPHAPEHTDMLGELRN
                     LAALAYEFGAVKPIWISEIGYATHLGPGGSSEWWSAAMLMRVYLTCWASGLVQKVFWY
                     DYRDDGTDPTYNESNFGILHHDWSPKMPYHGFKAMAHALEGFSPEGRVDLGEGPYVLK
                     FVRGDEVRYAAWTVGKNARVPVPAPADQVTVIRAWDAPLQAEARNGWVSVALDAAPVF
                     LVDSRRWTTS"
     gene            5765..>6013
                     /locus_tag="P5179_01375"
     CDS             5765..>6013
                     /locus_tag="P5179_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HRS93905.1"
                     /translation="MPIALGLDAGGTYTDAVLYDLSEDRILASAKAPTTPPSYIEGVA
                     GAIAGLPAAIRSRATYAALSTTLATNAIVEGRHAPAGLV"
     assembly_gap    6014..6113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6114..7719
                     /locus_tag="P5179_01380"
     CDS             <6114..7719
                     /locus_tag="P5179_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRS93906.1"
                     /translation="LTELPIVCGHEVAGVLNALVRAETAALNAGLIPIVQQFLHAVEG
                     VLSEVGLSAIPCFMVTGDGSLMATRVARLRPVETILSGPACSALGAARLSGVAEGIVV
                     DVGGTTTDIAVLRGGRPSMAPGGVSVGEWRAGVRSADVCTRGLGGDSSVAVQHGKLSV
                     GPQRVIPVSYAGHKCAALREEYRELLERVNSDGSPGGDWRLTNPTDAFLRLQSEARSA
                     LEDQEKAILDALESGPKTRWGLAKAVGYPHQSLLRTERLERWRLIQRVGLTPTDIWHV
                     TGQFQPWDKESARIATELAAARLGVSAEEVVARVGEIATQMLTRQVMHAYFGVPDEGN
                     HRGAHTLESHLTRIVLREVPRNGLGASLNLRPPIIGVGAPAHLFLSSVSDRLGGTFVL
                     PEGAPVANAVGAATGAVVATATALLRPEENGSIVCYTPEKRISTADRERAIAVARDVL
                     VPLVQEELRKKGAVAASVTVRVEHFGAEVKDVPDPIWWESTVTVRGMGHPFGADGNRD
                     DGLPDVVFQEEWVTPGLKEAEQRSTV"
     gene            7733..8119
                     /locus_tag="P5179_01385"
     CDS             7733..8119
                     /locus_tag="P5179_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93907.1"
                     /translation="MVSGDDPRSYRHPSNAHEGQANSRVLPPAGKWLAGRLRVCTKQA
                     VTVVTPEVRASSELPSFDPATGVPRASEGTDWVPLTEARSGREISSVVITAGPWGKKR
                     LARVIRTWEVLFNARAVIPETIECPG"
     gene            8146..9489
                     /gene="larC"
                     /locus_tag="P5179_01390"
     CDS             8146..9489
                     /gene="larC"
                     /locus_tag="P5179_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRS93908.1"
                     /translation="MRIAFFDCFAGIAGDMTVAALLDAGLDFEYLQAELRKLRVEGYE
                     LRKSTTRRHHIAATRFDVVIHGQVPYHTHATSADSLQHTRHEHPHDHGPDDHHTHGAG
                     RGHHNLDEGGPSSEERDHPVPLAAHVHRRLSDVVELIQTARLSQGVEKNAISVFKRLA
                     EAEASVHGTDPEEVHFHEVGAVDAIVDVVGTCLGIETMGIEEVHASAIRTGIGTIRAA
                     HGVMPVPAPGTLELLRGVPVDRTSLPYELVTPTGAAIITTFAKSFACPPVFVPETIGY
                     GAGSRDTQEIANLLRVEIGRTTGSIGEERALVVETTIDDMTPEVYGYVLERLFEAGAK
                     DVFLSPVIMKKNRPGIVVHVLVHPEQRERVISVLFGETSTLGVRISDVVRRVLPRRSE
                     TVETPWGPVRVKCAEWDGRTRITPEYDDCAAIARARSVPVLEVYEFVRKAADKSR"
     gene            9502..10284
                     /locus_tag="P5179_01395"
     CDS             9502..10284
                     /locus_tag="P5179_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93909.1"
                     /translation="MNTFRWMLLAGTIVVLVAAAPRIRPPEQPESVPLVHDPVRRLAS
                     RFDTFASVPGDPRGRIVYGTSRGYLHLLEFNGSRYQEIWVSPPLLSRIQKVLVADLGN
                     DGTFQIVATNTRGGLYVYDVNTFALLSRTQETQFRSIEAMTVADVDGDRRMEILFLSE
                     NRLFIFDAVHFVEKWRSDNLYRATDIAVGDVDGDGQQEIVLSSGHVLDAAFRNLEWEN
                     TTPFGEIVELGDVDGDGKLEVIGGTTQATTIWDVDERREKWD"
     gene            complement(10321..10584)
                     /locus_tag="P5179_01400"
     CDS             complement(10321..10584)
                     /locus_tag="P5179_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93910.1"
                     /translation="MDDIRFNARERLGEKRRAFVCRLTDGRVAIIYDGAGEDEPPATV
                     NDLDQAKRQVALLVPDIRVPLYEEHELKLVKQKLAGISSLSPV"
     gene            10919..11584
                     /locus_tag="P5179_01405"
     CDS             10919..11584
                     /locus_tag="P5179_01405"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRS93911.1"
                     /translation="MCNEKDCCEDQPVGLVRINEPAPEFEAVTTHGVLKLSDFKGKWV
                     VLFSHPADFTPVCTTEFMAFANHQEEFDKRGVQLIGLSIDSVHSHIAWVRSIESLFGV
                     KVKFPVIADLDMKVANAFGLIHPKASTTSAVRAVFVMDPDQLVKAIIYYPLTTGRSIP
                     EIIRLIDALQINAAKKVATPEGWKPGDKVIVPPPATTQAAEERMKATDMEVVDWYFSK
                     KSI"
     gene            11954..14464
                     /locus_tag="P5179_01410"
     CDS             11954..14464
                     /locus_tag="P5179_01410"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS93912.1"
                     /translation="MAEKNQWSASITFSDATTADGISLRQGVGRKTKPITDNRTVVPA
                     HVSGRACARAVPSGKKGSVGGYWRGTSESVDVQDGVFWLQIESPPPHSLREVYVTIDF
                     FESELGFIAVQYHRDGLEGFVHHVCPPGASKPWDSTWVNTLDTGSWQRATIRLVEPVF
                     DHSGFHDADLKIIGSGPLVLSTVRVSEERPEDFSDTDQMAHARRAEEHLSQSYPRASI
                     PVSIGNIGAIFEERDDSGVLSVPPWLPLYKGMGVTALQSYVRWSSIEREEGVWDWTFY
                     DWVVAEAKKFGLKWVAFIMIGPWYAMPKWWLATSNNYRNKCLEHGQESWVQSIWSPVQ
                     LDYVERFMRKFAEHYPEELIESIMVGISGDFGESTTNGVFTGDMYHTHVGYWCGEDVA
                     VADFRRTLREKYGSIGRLNEAWASSFGSFDEIRPVVRKDAPSDRAWIDQINWYCGQMT
                     WWMQRWGEICHEAMPNTVFYNAAGGAGDPPRAAHWTDQTRALKHTGMGHRATNEGCDY
                     AFNYVYTAWQGVGCRFYGLPFGNEPWGGDMAGNGVLGRVFNLVTQHGTNFWSYEGHLH
                     SPSGRLALQRGLPFLNGEYRRVNRVAVFYPWTHFVLADDHGFSEQGMRDRFWPQAEEL
                     RDIIDYDLIDDGLIKDGIMKDYDFLVILQGSTYEGPELERLYRWVENGGVLITHNLGV
                     PSTVEGDLSLGLRLMAFEPTVSPLEKELGGRMCRTGKGCVVMLPVCANRKGYFGDDRW
                     ADDHKDHPATHPAFWEMLTKTLANASKLDVGVEDYAIFDGVRDEVYGALMERAGVRGV
                     MFFSQVNSDVTKTVRLPGGRSKNVIVPAGQMVFAPFEG"
     gene            14501..15472
                     /locus_tag="P5179_01415"
     CDS             14501..15472
                     /locus_tag="P5179_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRS93913.1"
                     /translation="MQKQLLGLILAGGRGTRFGRLSERVPKPLLPAAGKPLIVHNIDG
                     LRRAGITEITIIISPDSSQVRSVLGNGHALGVSLEYVVQQEHLGTAHAISLAEERMGE
                     NRFLVCWSDNLSLYDMSDLIEHDRFFPADGTLAVYFTDNPRKGGAVITRGRDVVEFEE
                     KPEKPRSNLNLAGMYVLGRWVFECIRKTRLNSKEGEYYLPDAFQIAVKEGLSLHWTLV
                     GDWRMNVNTPEDLLVGNARLLEHNGTAIPPTTGCIISPPVHIPASVQIGKGALIGPFV
                     SLGEGCVIGKDVRIADAVVLSGAVIPDGAEIDRAIVDSNGDVYRVEG"
     gene            complement(15476..16468)
                     /locus_tag="P5179_01420"
     CDS             complement(15476..16468)
                     /locus_tag="P5179_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRS93914.1"
                     /translation="MSSTSLSETIGVAREDRAEKHEIRPRWTRYARTPLALAADLLFL
                     TRPVLLGPVWVVYGAGAVLVGSSPGIDLLFVSFVVAGVYIHNQLTDIPNDRANRKLFL
                     LSDGHVSHRAALTLTAMLWSAGILWSATQGIRSVLFALAIVLGLLYNGLRQSGWKSRP
                     LLGLVANALAHGVITFVAGFTAAGGTIFEGIVRSIPYAFAVAAVYLATTIVDENGDRL
                     TNKRTFCVVYGRAACTRWIAGLICAAAVTAAANGDGWMAAAAIVSLVPAFLLLRAPAV
                     GNAQRAAVIAVSVLALAVAVRWTTLFVLAAATFAATRYYYRLRFGVVYPTLGSG"
     gene            complement(16485..18095)
                     /locus_tag="P5179_01425"
     CDS             complement(16485..18095)
                     /locus_tag="P5179_01425"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRS93915.1"
                     /translation="MPNCRFNDQVLPIVVGKTLFEHADTNPAIRVPSSCNRVGTCHEC
                     IVQVHEGIEALTPRTDSEQFLSGNYRLACQAKVIRDDMEINVSTLKRKPKILTASEVL
                     DVPLDPLTRRSGDRVVRGNNDIDAFDGEVLGLAIDVGTTTVVAQLVDLTNGKRKLTAA
                     FENPQIFGGSNVLHRINYANNDPDHELQRVLIGHLNGELRRMGKRRHIYEAVVTGNPT
                     MRDLFFGLDVHSIGVKPFKSITEHEMLRGERTGTWLNASPRDLGLFMNGRGLVYGAPL
                     IACHVGADTAAGVLATRLFERERPSMLVDMGTNTEVVVGNKHRLIAASCPAGPAFEGS
                     GLRCGMPGLEGAVESFRLEDGNPVYSVIGDVTPRGICGSGVVDILAELTRNGIVDTVG
                     RFVDGRTEFVIDREQNIAVDRQDLSQLAQAKAANYAGQQILLRTFGIDWDDLEFLFFS
                     GGFANYLNVPNAQAIGLIPPIDPAKVMKVGNTALEGAAQMLINKGLRERIEQVVLTIE
                     HIELEREPDFFDMYVEGCLLEPMGRLKG"
     gene            18209..19108
                     /locus_tag="P5179_01430"
     CDS             18209..19108
                     /locus_tag="P5179_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRS93916.1"
                     /translation="MKSKIEHAAESGHPRRFPLPVAATCVYVVLNICRVLVSTVSIGH
                     TSMEQLLSPLRSINVVLVRPESPGNVGAVARVIRNFGLGELRIVGSDIDTDGPAARLA
                     HRSLGVLKGARFYEDVDSAVADAEWIIGTSGRLRSGRPMPATVRSAASRIVAQCSHGA
                     GAILFGPESDGLSLRDLAHCDELIRVPQRRDAPALNLAQAVAVVGAELFQAALVPGNA
                     PVPPSVTREARKRFVRRLKALSTHCGLSVRNRPEETLEELEGIFVRRRFEPHEMAVLE
                     LWLSQLEWFTGMDQGSNGRGTAE"
     gene            19114..>19711
                     /locus_tag="P5179_01435"
     CDS             19114..>19711
                     /locus_tag="P5179_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93917.1"
                     /translation="MRRRSSFLLASLVFTGAAACFASQWHEISLPASYERLHCMAMLT
                     PESGWAGGQGGLILRCEGGQWRKVTAPTRSDVIAMAFDDEENGWVAGFDAASQKSLLF
                     RYQRGRWTPIPVPVGLIVTAIASDGRGTLFVFGQGGRAFRLRQRVWLETGTPGYRTML
                     AAVSLPDGAVWAVGEFGSAWRWNGSSWQGTQTPVRSHLH"
BASE COUNT         3968 a         5572 c         6063 g         4008 t
ORIGIN      
        1 cccgcctttg ccatggtgga cactctgagt ttgccggttg ttggcgcaat tgccgcgggt
       61 tcaccgcggg aggcaattca gcagacggac aggtgggtgg aacgactgga ggatttgcta
      121 ccggtccggc cgggggattt cctcctgacg gtgaacgggg agtcgatgat cggggacggg
      181 attctgcccg gggacctggt gctcattcgt ccacaggagg tcgtggaacc gggccagata
      241 gccgcggtga tggtgcgcaa tgacgaagcg accctgaaac ggttctaccc gcagccacct
      301 tcgcatgttc gcctcgtccc atccaatccc acaatggatg acatggtgta ccctgcagag
      361 gaagtgcgcg tgattggcaa gtatcagggt ctgatacgca ccggccacca tggtcgccag
      421 tagccgcgat catcagaggg gccttacaat gccggttaca ctccattcga cagacgagcg
      481 tactgaagtg caggtgcgtg tcgacgacag cgcaatggtc gatcctttgc agctccgtcg
      541 ggatgtcatc gcggcattgc aacgcagcgc tcgcaaggaa atcacgttga gcctcgaagg
      601 cgttgcgaag atagacacca ggctcttatc cgctatcaag atggccgtga ccgaagcccg
      661 tattcgcagg cgccgcatca tcctggtgtc agcgcccgcg gcgattcgtt tcgccctctc
      721 gggaaccagc ttccgtgtgc gatgggctgc cggcctcttt ccgcttgaca ccgaatctcc
      781 tgccggggcg ctgattctca accggtacac ttcgcgcgtt tccgccgtgt aaatcgacgg
      841 cgaggacacc ggaccgatgg acgcacaatc gcttgccaaa accgttcaaa cccccttcaa
      901 acacggatcg ctcatcctgt cgccctcttt ccgcccgggc gatttcgaca gccacggcgt
      961 tgattgcccg ttcgtgttcc gtcatgagga tcgtttcctc atgacgtata tcgggtttga
     1021 cggccttggg tatcgaactg gccttgcgtg gtctgacgat cttctcgtct ggcacaggga
     1081 gggaatgatc ctcgaccgcg gaatcgccgg atcggtgacc cagcacaacg ctgctctgac
     1141 gtggatcttg cgggacaacg atctttttgg aactggatgc ctccgccaga ttgatggtcg
     1201 gtacgtcggg acttatcacg cctaccccgc gcaggggtat gaaagtggac ccgcggtgat
     1261 cggcatctgc tccagcacga atctgcgcga ctgggaaatc ggtgagccct gtctgattcc
     1321 cgcggaaggg gaggaatggg aacgtggggg actttataaa tcctgcctca tcgaattcca
     1381 gaggaggtac tatctttttt acaatgcgaa gactgtcggg gtaccctgga tcgaacagac
     1441 cggcgtagca atctcacgcg acctcatgcg gtgggaacga tacggcggga acccgattct
     1501 gcgcacagga gcggaaggcg cgtttgatga acgattcgcc agcgacccct gtgttctgcg
     1561 cgttggcgac gcatgggcga tgttctattt cgggctcggt cgcgatggcc acgcacgaga
     1621 tggcgtcgcg ttctcccgcg atctgctcca ctgggagaaa tccggttctg ttctcgttga
     1681 tgtgggccca ccgggggcgg tcgattcgcg gtacgcccac aagccatcaa tgttctggca
     1741 caggaatcgg ctgttccact tctattgcgc cgtctcacca aggaacgaac aagccgctgt
     1801 cggtgccgtc catacgagtg aaactcgagg tatcagcgta gcattttcct gaaacggctc
     1861 ccgtcccttc tttgatttcc ccacggaccg cgatctgata accctctccc cgccgttttt
     1921 ctcgcgcccc cattgacttc ggccagcgat tgttgaattt tttaaagtta atattcttgt
     1981 gactccctca gtgtttgcgt gtctgcagct atcacctcac gggctttcca cgagtggagt
     2041 aaccatgccg cgtcgccccg cgttataccc acggcagaaa tgggaatggg ccaaggacct
     2101 tcccgtttcc gacgcgccgt gggaggatct ccttcgcctc ccggacattc gttttctgac
     2161 cgacaatcag gtgagcctct ggacgtctcc tgattctata ggcctcgaga aagtgtggtt
     2221 ctacccggtt ccgcgggctc cccacctcga ggtcgaacgc tttgacagcg ggagaatcgc
     2281 acagagctcg gtccacgttc ttcgcggccc ccggggcgca ttgatggcag gtggaaaacc
     2341 tgtcacctgg gatacgcaga atctccagtg gtctcctgcg gagataacgc gaacgtttgt
     2401 ctccccggaa gtcacgctcc aggaaacggt gacgatcgtt gaggacgcca tcgtggtctg
     2461 gctgaggccg gttgagggaa cctccgaatt acccttgtcc gcggcgtggt cttcctcggt
     2521 ggaaagaaca ctctcgggtt cctggcgcat gcacgacggg gcccgggtgt gcatgcacga
     2581 cgacggcatt gcccacggat gggtctggtt cggttcagag gtaaccaact ggcaagggaa
     2641 agcagcctcg gggaagttcg gttgggaagc ttccataccg ccacaggggg gcgtgcttgt
     2701 gctgctgctc ggttacgacc aggcagcagt cgaacgccgg ctcgaggcga tgatcgcgca
     2761 gtgctcgaat cggccgacgg ctgacatcgc ggaattgttc ctgacgaagg cgcgtgacgc
     2821 atggcagtgg tacatgaagc gcatggttcc cgggctcaaa ggtgcggaag agtgggtgag
     2881 gcggctttac tactaccaga cggcattgca caagatcaac ctttacgata tcccctacga
     2941 acccttcgtg tacccgtata catgtccctg gaaaaccgga gccgtgtggc agtggtcatg
     3001 gaatactccc atgaacagca tcgccgagcg ctggctgaat gatccggcgt gggccgagtc
     3061 gggtatcgaa ttgctgagag aaaatggcgg ggcgctgaac ataggtgctt cacttcacag
     3121 actccgcaaa ccgcggcagt tcagggatgt caacgaatac ctgcccgcat tgcaggacgc
     3181 catcggtggc gtgaaaaacc tcccggcgac tgcgcgtcag tttgactggg cgttcattat
     3241 gccccacaca acgcctctcg gcgtgcacgg aatctgggaa gtgttccgcc gaacgggcga
     3301 tgaggattac ctccgtcgcc agcttcccga aatggttgcc tacgaagctc gcctgacatc
     3361 gcatgacgcg gacggagacg gccttgtgga atacgagggg atggttgatg aatacgacta
     3421 ctccctgcgc tggcgcactg ccgtgaacgg gtacaagaaa ggcaccgaat ctctgatccg
     3481 gtttgacagg ccgctggaac tgatcgacat aaacgcgcaa ctgtgcctcc ttcgggaaga
     3541 cataattgaa gcggcgggga tagttggcga caggaacctc gcaaaacgga ttcgccagcg
     3601 tctcaacaag acggcgaggg caatcaacga tctcatgtgg gatgaaacgc ggggctggta
     3661 cggggacctc gatgccgtga cgcacgagct caccggcgtg tgttcggtgg cggcgttctc
     3721 ggcgctgatg tccggagtcg cgccggcgga ccgtgcggaa cgaatggtcc gtctcctgca
     3781 ggatcctgcc gcatttggca cgccgtatcc ggttccgtcg gtcgccgcag acacaccgga
     3841 tctggatccg ggccacatca cgtacggggg tgatgtgctg atcaccagcg gggtctggct
     3901 tactgtcgtc gctctggtga ggtatggctt cctcgaggag gcgaggagaa ttatctggaa
     3961 ggcgctgcgc atgctgggcg aaaacgcgcc gacgtcctcg tactcctaca actccatgac
     4021 gggcgcgccg aacatggacc cgcacacctt ctgcagccag tccgccatta cgctcgacct
     4081 gttcgtgcgc ttcgtggtcg ggttcgtccc ccgatatgac gacatcatcg agtgctggcc
     4141 atttgcgctc ccgaccgaat gggaacacct ggaattcggt cccatgacgt ggcgtggcga
     4201 tgttgacgtc accgtggtgt gggattcgga atctggctac accgtgcggg ccggcagaag
     4261 cgagttcacg gcgcgcgagc cccgacacct ctggctggcg cttgaccagc gggatagact
     4321 ggtcgagctt cctgatccgt cggtcagtgg catgccagtt atgacgtagt ccaccgccgg
     4381 ctgtccacaa gaaatactgg agccgcgtcc agggcgactg acacccagcc gtttcgtgct
     4441 tctgcctgaa gcggcgcatc ccacgcccgg ataacggtca cctgatccgc cggtgccgga
     4501 accggcactc gcgcgttctt ccccacggtc cacgccgcgt agcggacctc gtcaccccga
     4561 acgaatttca gcacgtacgg cccttcgcca agatcaactc gcccttcggg tgaaaaacct
     4621 tccagcgcat gtgccatcgc cttgaacccg tggtatggca tcttgggtga ccagtcgtgg
     4681 tgcagaatac caaaattgct ctcgttgtat gtcgggtcgg ttccgtcatc gcggtaatcg
     4741 taccagaaaa ccttctgcac gagcccgctg gcccaacacg tgaggtagac tcgcataagc
     4801 atcgccgcgg accaccactc actgcttcca ccgggtccca gatgggttgc gtatccgatt
     4861 tcactgatcc agatgggctt gaccgccccg aactcgtacg ccagcgcggc aaggtttcgc
     4921 agctcgccca gcatgtcggt gtgttcaggg gcgtgaggat aacggtacgg gtgtactcca
     4981 ataatgtcgg tggtatccca cccaccctct tcaaacacca cgcggtcaaa agccaggtcc
     5041 accatggatg tgttgcaccc gactatccaa cacgtcgggt cggccgcacg ggcagcctcg
     5101 gcgccggcca cgaggagctt cgcgtattcc cgtggattcg gttttggctg ccagaatccg
     5161 gaaatgttgg gttcattcca catctgccac acatggattg tctctcggta ccgctccact
     5221 gttctgaaca cgtattcctt ccaggcgctc agatctggta agctcacgac ggatggtttt
     5281 ccctcagccg ttctgtgggt tgccgcccac ggcgctgaat agcagagcag gcccagcgta
     5341 tgaatgccgt gttcccgtgt caaacgaatg gcacggtcaa aggcttccca cttccagact
     5401 ccgcgttcgg gttggattcc tccccagccg agttcatctc gcgtccactt gatccccgcg
     5461 tcccgcgcga gcccaagcgc cctcagtgtc gtttcgccag acgttctgtg acacgggtag
     5521 atgcccattc cgaacggcga cgggtcgagt tgcacttttg cacccatggc aagcccccta
     5581 cgggaaatgt tgcgtcgaaa cgagaatatg gttcagagag tgcatcaggt ttgcatccga
     5641 tgcaaccccg tgcgctggga ggcaccttct cattgtggtg aggctcgggg attgggtacc
     5701 tttgttattc ggaaccgatt cagtggcgtt gacaacaatc gcgtaacgcg ggacggaaac
     5761 atatatgccg atcgcacttg gtctggacgc gggtggaaca tacacggatg cagtgcttta
     5821 cgacctttcg gaagaccgca ttctggcttc cgcgaaggcg ccgacaacgc ccccctccta
     5881 catcgaaggc gtggcgggag ccattgccgg tttgcctgcc gcgatccggt ccagggcaac
     5941 gtacgccgcg ctctcgacca cgctggctac gaacgcgatt gtcgagggac ggcatgcccc
     6001 tgcggggctg gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactgacg
     6121 gaactcccga ttgtgtgcgg acacgaagtt gcgggcgtac tgaatgccct tgtccgtgcc
     6181 gaaacggccg cgttgaacgc cggactgatt cccatcgtcc agcagttcct gcacgcggtg
     6241 gagggtgtgc tgtctgaggt gggtctctcc gccattccgt gtttcatggt aacgggcgat
     6301 ggcagcctga tggccacgag ggtggcccga ctgaggccgg tggaaacgat tctgtccggc
     6361 cctgcgtgca gcgccctggg tgccgcacgg ctctccggtg ttgcggaggg aatcgttgtc
     6421 gatgtcggtg gcacaacaac cgatatcgcc gtattgcggg gtggacgacc gtccatggcc
     6481 ccgggtggtg tctccgtagg cgaatggcgg gccggagtcc gttcggcgga tgtctgcact
     6541 cgcggcctgg gcggggatag ctcggtcgca gttcagcacg ggaaactttc cgtcgggcct
     6601 cagcgggtga tacctgtttc ctatgccggg cacaagtgcg cggctcttcg tgaagaatac
     6661 agagaattgc ttgaacgagt gaactcagac ggttcgccgg gtggagactg gcgattgacc
     6721 aacccgacgg acgcgttcct gcgactccaa tcggaagcac ggtccgctct cgaggaccag
     6781 gaaaaggcta ttctggatgc actggaaagc ggcccgaaaa cgcgctgggg tctggcgaag
     6841 gctgtcggat acccgcacca gtcgcttctc cgaacggaac gcctggaacg atggaggttg
     6901 atccaacggg tgggccttac gccaacagac atctggcacg tcacgggtca gtttcaaccc
     6961 tgggataagg aatcggctcg catcgcaacc gagcttgctg cggcacggct cggtgtcagt
     7021 gcggaggagg ttgtcgcccg cgtcggggaa atcgccacgc agatgctcac acgccaggtc
     7081 atgcatgcat atttcggtgt gccggacgag ggcaaccacc ggggtgcgca cacgctggaa
     7141 tcgcacctca ccaggatcgt tcttcgtgag gtaccccgaa acgggcttgg cgcttcactc
     7201 aacctgcgcc cgcccattat cggtgtgggg gcgccggcgc atctgtttct ttcctcagtg
     7261 tcggaccggc ttggcggaac ctttgttctt ccggaaggtg ctccggttgc gaacgccgtg
     7321 ggagctgcca ccggtgcggt cgtcgcgacg gctacggcgc ttttgcgccc ggaggaaaat
     7381 gggagcatcg tctgttatac gccggaaaaa cgcatttcaa cagcggaccg ggaacgtgcg
     7441 attgccgtgg cgcgagatgt gttggttcca ctggttcagg aggaactgag aaaaaagggc
     7501 gcggtggccg cctcagtgac cgttcgcgta gaacatttcg gcgccgaggt gaaggatgtt
     7561 cccgatccca tctggtggga atcaactgtt acggtccgcg ggatgggcca cccgttcggc
     7621 gccgatggga accgggacga cgggcttccg gacgtcgtgt tccaggagga atgggtaaca
     7681 ccgggtctca aggaagcgga acaacgttca accgtatgag caggagacgc tgatggtatc
     7741 aggtgacgac cctcggagtt accggcatcc gtccaacgcc catgaagggc aggcaaacag
     7801 cagggtgctt ccccctgctg gcaagtggct tgcaggacga ctgagagttt gcacgaagca
     7861 agccgtgacg gtagtaacac cagaagtacg tgccagttcg gagctgcctt ctttcgatcc
     7921 ggcaaccggt gttccgcgag cgtccgaggg cacggactgg gtgccgctca cggaagcccg
     7981 ttcgggcagg gagatttctt ctgtcgtcat tacggctgga ccctggggga aaaagcggct
     8041 ggcgcgcgta ataaggacgt gggaagtgct gttcaacgcg cgcgcagtga ttcccgaaac
     8101 tatagagtgt cccggttaga cgcggagaac gaggcagggc gcccgatgag gatagcgttt
     8161 ttcgattgct tcgccggaat tgccggtgat atgacggtag ccgcgcttct tgacgccggc
     8221 ctcgatttcg aatacctgca ggccgaactt cgaaagctcc gcgtcgaagg gtacgagctc
     8281 cggaaatcaa caacccggcg acatcatatc gcggcaacca ggttcgatgt tgttatccac
     8341 gggcaggtgc cctaccacac ccacgccacc tccgccgatt cccttcaaca cactcgccac
     8401 gagcatccgc atgaccatgg gcccgatgat caccacacgc acggagctgg ccggggacat
     8461 cacaatctgg acgaaggtgg gccatcctcg gaagaacggg accatccggt tccccttgcg
     8521 gcgcatgtcc accgccgtct ttcagacgtg gtagagctca ttcagaccgc cagactctct
     8581 cagggtgtgg aaaaaaacgc gatcagcgtt ttcaaacggc ttgcagaggc ggaggccagc
     8641 gttcacggaa ctgacccgga agaggttcat ttccacgagg tcggagcggt tgatgcgata
     8701 gttgatgtgg taggaacctg cctcggaatc gaaacgatgg ggatagagga ggttcacgcg
     8761 tcggcaatcc gcaccggcat cgggacgata cgggccgcgc atggtgtcat gccggttccc
     8821 gcgccgggaa cgttggagtt gctccgcggt gtgccggtcg accgaacctc gttaccgtat
     8881 gagctcgtca ctcctaccgg tgcggcgatc attacgacgt ttgccaaaag cttcgcgtgc
     8941 cctcctgtgt ttgtgccgga aacgatcggc tacggggccg ggagccgtga tacgcaggag
     9001 atcgcgaatc tcctgcgcgt tgaaataggc cggacaacag gttctatcgg ggaagagcgc
     9061 gcgcttgtcg tcgagaccac aattgatgac atgacgccgg aagtgtacgg gtacgttctt
     9121 gaacgccttt ttgaagccgg tgcaaaggac gtgttcctgt cgccggtcat catgaaaaag
     9181 aaccggccgg ggatcgtcgt ccacgttctc gtgcacccgg agcaacggga aagagtgatt
     9241 tcggtcctgt tcggggaaac atcaacgctt ggcgtgagga tatccgacgt tgtccggcgc
     9301 gtcctccccc gccgaagtga gaccgtcgaa acaccctggg gacctgtccg cgtgaaatgt
     9361 gcggaatggg atggacggac gcggatcacg cctgaatatg acgactgcgc cgcgatcgcg
     9421 cgtgccagaa gcgtcccggt tctggaggta tacgaattcg ttcgcaaagc cgccgacaaa
     9481 tcgcggtaag ggggaggaac catgaatacg ttcagatgga tgctccttgc ggggacgatc
     9541 gtcgttctgg ttgctgcagc cccgcggatt cgaccaccgg agcagcctga aagcgtgcct
     9601 cttgtgcacg accccgtcag gcggcttgca tcgcgattcg acacgttcgc ttcagtcccg
     9661 ggggatccga gaggccgcat cgtgtacgga acgtcgcgtg ggtacctcca tcttctggag
     9721 ttcaacggga gccggtacca ggagatatgg gtgagtccac ctcttctgtc ccgaatccag
     9781 aaggttttgg tcgctgatct cggcaacgac ggaacctttc agatcgtcgc cacaaatacc
     9841 cggggaggcc tgtacgtcta tgacgtgaac acgttcgcat tgctttcccg aacgcaggaa
     9901 acgcagttcc ggtccatcga agcaatgacg gttgccgacg tggacggcga ccgaagaatg
     9961 gagatcctgt ttctgagcga gaacaggttg ttcattttcg acgcggttca tttcgtcgag
    10021 aaatggcgct ccgacaacct gtaccgtgca acagatatcg cggtaggaga cgtggatggc
    10081 gacgggcaac aggaaatcgt gctgagttcc gggcacgttc tggatgcggc attccgcaat
    10141 ctggaatggg agaacacaac tccattcggg gaaatcgttg aactcgggga cgtggacggc
    10201 gacggtaagc ttgaggtgat agggggaacg acgcaggcaa caacaatatg ggatgtagac
    10261 gaacgccgcg agaaatggga ttgattccgt aattctgaaa ggcggccggt gtgagttgcc
    10321 tcacaccgga ctcagggagg agatacctgc aagtttctgc ttcactagtt tcagttcgtg
    10381 ctcttcatac aacggcacgc gaatatcggg aaccagaagc gccacctgcc gtttggcctg
    10441 atcgaggtcg tttactgtgg cagggggttc gtcttctccc gcgccatcat agatgatggc
    10501 gacacgcccg tctgtcaatc ggcagacgaa cgcgcggcgt ttttcgccaa gacgctcgcg
    10561 agcattgaac cttatgtcat ccacttcctc aacctccgcc ccccgattcc ttcaatcaat
    10621 tacacccaac ccaccgtggc ggttccatat gccgcggcga aagaacgacg tcatttcggc
    10681 cattaccggg ccgataatcc gttgttccgg gcccaattcc acggcaacat cctgtaatcc
    10741 aagtgtaata tatacgttag cgctcctgct gtcaatccga actcacaggc tgcgtgtccg
    10801 gtgtgttctc ggagcttgcc agctatcaga atttgtgaga ccttatctat ggacagacga
    10861 gatcgcaact caactccgag gggggcttga gagcggtcca tgccaaggag aagcgaagat
    10921 gtgtaacgaa aaggattgct gcgaggatca gccggtgggt ctggtgagaa tcaacgaacc
    10981 ggccccggaa ttcgaggcgg tgactaccca cggagtgctg aagttgagcg acttcaaggg
    11041 caagtgggtg gtacttttca gccaccccgc tgattttact cctgtgtgca caaccgagtt
    11101 catggcgttt gccaaccatc aggaagagtt cgacaagcgt ggcgttcagc tcatcggcct
    11161 cagcatcgac tctgtccatt cgcatatcgc ctgggttcgg agcatcgaat cgctcttcgg
    11221 tgtcaaagtg aagttcccgg tcatcgccga cctcgacatg aaggttgcca acgcattcgg
    11281 cctgatacat ccgaaggcct ccacaacgag cgcggtccgt gcggtgtttg taatggatcc
    11341 cgatcagttg gtgaaagcaa tcatctacta cccgctcact accggtagga gcatcccgga
    11401 aatcatccgc ctgattgatg cgctgcagat caacgcagcc aaaaaggttg cgacgcccga
    11461 agggtggaag ccgggagaca aggttattgt cccgccgccg gccaccacac aggcagccga
    11521 agagcgcatg aaagcgacgg acatggaagt cgtggattgg tacttcagca aaaagagcat
    11581 ctgaagaagt caaagaaccc ggtagaaaaa ggcgctggcg agcgtgatgt ctcgccggcg
    11641 cctttgaatt tccaactcca ctccctgcac gctgaactta cccgcgctaa ccgtcattcc
    11701 gcgagggttc tcttgcgact caacaccccg cagaatccgt cgccacactc gtcaggccaa
    11761 aggggccatt acccccgcaa aggtgtcttg ttaccttctc tggaacatgg gtcacctgcg
    11821 ccggtatgcg gcgcagtatc gaactcaaat caagggtgta ttcgcttcca ccaattgacg
    11881 gtaccggcgc gcaggttccc tgcatcaccc cggcgccgtt cgcctctgag tttcacttga
    11941 tgaaggaact atcatggcag agaagaacca gtggagtgcg tccatcacat tctccgacgc
    12001 gacgacagca gacggaataa gccttcgcca gggcgttggc agaaaaacga agcccataac
    12061 agacaaccgt accgttgtgc cggcgcatgt atccggaagg gcgtgcgccc gcgcggttcc
    12121 ttctggcaaa aaagggtcgg tgggaggtta ctggcgtggc acgagtgaga gtgttgatgt
    12181 tcaggacggc gtcttctggc ttcagatcga aagtccgccg ccccattccc ttcgcgaggt
    12241 gtatgtcacg attgactttt tcgaaagcga actcgggttc atcgcggtgc agtaccaccg
    12301 ggacgggctt gagggttttg tgcaccacgt gtgcccgccc ggtgcgtcca agccatggga
    12361 ctcaacctgg gtcaatactc tcgatacagg atcctggcag cgggcgacga tacggcttgt
    12421 ggagccagtt tttgaccact cgggttttca cgacgctgac ctcaagataa tcgggtcggg
    12481 tccgcttgtg ctttcgacgg tacgggtttc cgaagaacgt cctgaggact tctcagatac
    12541 cgaccagatg gctcacgcac gccgtgcaga agaacacttg agccagtcct accccagggc
    12601 ctcaattccc gtttcgatcg gaaacatagg agcgattttc gaggaacggg acgattcggg
    12661 cgtgctgagc gttccgccgt ggcttcccct gtacaaaggc atgggtgtaa ctgcgctcca
    12721 gtcctatgtc cgctggtcaa gtatcgagcg cgaagaaggc gtctgggatt ggacttttta
    12781 cgactgggtg gtggccgaag cgaagaaatt cggactcaaa tgggttgcat ttatcatgat
    12841 cgggccatgg tacgccatgc cgaagtggtg gctcgcgacg tcgaacaatt accgcaataa
    12901 atgcctggaa cacggacagg aaagctgggt gcagtctatc tggagcccgg ttcagctcga
    12961 ttacgtggaa cgattcatgc ggaaattcgc cgagcattac ccggaggaac tcattgaatc
    13021 tatcatggtg ggcatttccg gcgatttcgg tgagtcgacc acgaacggtg tcttcacggg
    13081 cgacatgtac catacgcacg tggggtactg gtgcggcgaa gatgttgccg ttgctgactt
    13141 ccgcaggacc ttgagggaga aatacggcag catcggacgg ttgaacgaag cgtgggcttc
    13201 atcgttcgga tcgttcgacg aaatccgccc cgttgtgcgc aaagacgccc cgtccgacag
    13261 ggcgtggatc gatcaaatca actggtattg cggtcagatg acgtggtgga tgcagcgctg
    13321 gggggagatc tgccatgagg cgatgcccaa caccgttttc tacaatgcgg cgggtggagc
    13381 aggtgatcca ccgagagctg cgcactggac tgaccagaca cgcgcgttga aacacacagg
    13441 catgggacat cgtgcaacaa acgagggatg tgactacgcc ttcaactacg tttacacggc
    13501 gtggcaaggg gtgggatgcc gtttctatgg tctgccgttc ggaaacgaac cgtggggcgg
    13561 agacatggcc gggaacggcg ttctcggaag ggttttcaac ctggtaacgc aacacgggac
    13621 gaacttctgg tcgtatgagg ggcacctcca ttcaccttcg ggacgccttg cgctccaacg
    13681 gggtcttccg tttctcaacg gggagtaccg tcgtgtgaac agggttgccg tgttctaccc
    13741 gtggacacat ttcgttcttg ccgacgatca cggtttttcg gagcagggga tgcgggacag
    13801 gttctggccg caggcggaag aactgaggga catcatcgac tacgacctga ttgacgatgg
    13861 cctgataaaa gacgggatta tgaaggatta cgattttctg gtgattcttc agggctcgac
    13921 gtacgaggga ccggaacttg agaggctgta ccggtgggtg gagaacgggg gtgttctgat
    13981 tacgcacaac ctcggtgttc cctccaccgt ggaaggagac ctgtctctgg gattgcgcct
    14041 tatggctttc gagccgacgg tcagtccact tgagaaggaa ctgggcggcc ggatgtgccg
    14101 gacgggcaag gggtgcgtcg tcatgcttcc cgtgtgcgca aatcgcaaag ggtactttgg
    14161 ggatgatcgc tgggcagacg accacaagga ccacccggca actcaccctg cgttctggga
    14221 gatgcttaca aaaacgctgg cgaacgcgtc gaaactggat gtgggcgttg aggattacgc
    14281 gatcttcgat ggcgtacggg acgaagtgta cggcgctctt atggaaagag caggcgtacg
    14341 aggcgtgatg ttcttcagtc aggtgaattc ggacgtgacg aaaaccgtcc gactccccgg
    14401 cggaagatcg aagaacgtga ttgtcccggc agggcagatg gtcttcgcgc cattcgaggg
    14461 atgatccgcg gcacgtgaca ggacgtgatg gatgcgagaa gtgcagaaac aacttctggg
    14521 actgattctg gcgggcgggc gtggcacgcg gttcgggagg ctctcggaac gcgtgccaaa
    14581 accgctcctg ccagccgccg gcaaaccgct catcgtccac aacatcgacg gtcttcgccg
    14641 cgcggggatt accgagatca cgatcatcat ttcgccggac tccagccagg tgcgcagtgt
    14701 tctcgggaac gggcacgcct tgggggtgtc gctggagtac gtggtgcaac aggagcacct
    14761 tggaactgcc catgcgataa gtctcgccga agagcggatg ggtgagaacc ggtttctcgt
    14821 ctgctggtcg gacaatctga gtctgtatga catgtcagac ctgatcgagc acgaccggtt
    14881 cttccctgca gacggcaccc tcgcggttta cttcacggac aatccccgaa aaggaggagc
    14941 cgtgatcacg cgcggacggg atgttgtgga gttcgaagag aagccggaga aaccgcgcag
    15001 caatctgaat ctggcgggaa tgtacgtgct cggacggtgg gtgttcgagt gcatacgcaa
    15061 gacgcgcctg aactcgaaag agggggaata ctacctgccc gatgcgtttc agattgcggt
    15121 gaaggaaggt ctgagtctcc actggacgct cgttggcgac tggcggatga acgtgaacac
    15181 gcctgaggat ctgctcgttg gaaatgcgcg ccttctggag cacaacggaa cggcaattcc
    15241 gccgacaacc ggctgtatca tctcccctcc cgtgcacata cccgcgagcg tccagatcgg
    15301 gaagggcgct ttgatcgggc cgttcgtgag tctgggtgag gggtgcgtca tcgggaaaga
    15361 cgtcaggata gcggatgccg tcgtgctcag tggcgcggtc ataccggacg gagcggagat
    15421 cgacagagca attgtagatt ccaacggcga tgtgtaccgc gtggaaggct gaaactcagc
    15481 cgctgcccaa tgtcgggtac accacaccga agcggaggcg gtaatagtat cttgtggcgg
    15541 cgaatgttgc tgccgccagg acgaacagag ttgtccagcg cacggcaacg gccagggcga
    15601 gaacgctcac ggcaataacg gcagcgcgct gcgcgttccc gacagcaggt gcccgcaaga
    15661 ggaggaacgc gggaacaagt gacacaatgg cagccgcagc catccaccca tccccgttcg
    15721 ctgccgcggt cactgcggcc gcacagatca atccagcgat ccaccgcgtg cacgcagccc
    15781 ggccgtagac gacgcaaaat gtgcgtttgt tcgtcaatct gtcgccgttt tcgtcgacga
    15841 tagtagtcgc gagatatacc gcagcaacgg cgaaggcata gggtattgaa cgcacgatgc
    15901 cttcgaaaat ggttccccct gcggcagtga agccggcgac gaacgtaatc actccgtgtg
    15961 cgagagcgtt cgccacgagc cccagcaacg gacgcgattt ccaccccgac tgccgcaatc
    16021 cgttgtacaa aagccccagt acaatcgcca aagcgaacag aacggacctg atcccctgcg
    16081 tcgccgacca caggattccg gccgaccaca gcattgcggt aagtgtcaaa gcagccctgt
    16141 ggctcacgtg tccgtcggag aggagaaaca gctttctgtt tgctctgtcg tttggaatgt
    16201 cggtgagctg gttatggatg tacaccccgg cgactacgaa ggatacgaag aggagatcaa
    16261 tcccggggga acttcccacc agaacggccc cggcgccgta cactacccac acaggcccca
    16321 gcaggacagg tcgtgtgaga aaaaggaggt ctgccgcaag cgccagcgga gtccgcgcat
    16381 accgggtcca acgcgggcgg atttcgtgct tttctgcgcg atcttcccgc gcgaccccaa
    16441 tcgtttcgga cagactcgtg gagctcattt cgggtttttc gatgtcagcc cttcaatcgg
    16501 cccataggtt ccagcagaca accctcgaca tacatgtcga aaaagtcggg ttcacgttcc
    16561 aactcaatgt gctcgatggt caagacgacc tgttcgatcc gttcgcgcag gcccttgttg
    16621 atcagcatct gagcggcacc ctccagtgcc gtattgccca ccttcatgac ctttgccgga
    16681 tcgatcggcg ggatgaggcc aatagcctga gcgttgggaa cgttcagata gttggcgaag
    16741 cccccagaga aaaacaggaa ctcaagatca tcccaatcga tgccgaacgt tctgaggagt
    16801 atctgctgcc cggcatagtt ggctgctttc gcctgtgcaa gctgactcag gtcctgtcga
    16861 tccactgcga tgttctgctc tcggtcaata acaaactccg ttcggccgtc gacgaatctg
    16921 ccgacggtgt caacgatgcc gttgcgcgtg agctctgcga gaatgtccac gacgccggag
    16981 ccgcagattc cacgaggggt gacgtcgccg ataacggaat agacgggatt tccatcctcc
    17041 aggcggaacg attccaccgc gccttcgagc cccggcattc cgcaacggag cccgctccct
    17101 tcgaacgcgg gtcctgccgg gcacgaagcg gcaatgagcc gatgtttgtt tcctaccacc
    17161 acttcggtat tggtgcccat gtcgacgagc atggaagggc gctctcgttc gaacaggcgg
    17221 gtagcgagga ctcccgccgc agtgtcagcc ccgacatggc acgcgataag gggagctccg
    17281 tacacgaggc cccggccgtt catgaagagg ccaagatcgc gtggcgacgc gttcagccac
    17341 gtgcccgtgc gctcaccccg aagcatctca tgctcggtga tggatttgaa cggtttcacg
    17401 cctatgctgt ggacatccaa tccgaagaat agatcccgca tggtggggtt gcctgtcacc
    17461 actgcctcat agatatggcg ccgcttgccc atgcgtcgca gctcgccgtt caggtgccct
    17521 atcaaaacgc gctggagttc gtggtcaggg tcgttgttgg cgtagttgat ccggtggagt
    17581 acgttacttc ctccgaaaat ctgggggttt tcgaatgccg cggtgagctt tcgtttcccg
    17641 ttggtgaggt cgacaagttg ggcgacaacg gtcgtcgttc ccacgtcgat ggcgagtccc
    17701 agcacttcgc catcgaatgc atcgatgtcg ttgttcccgc gaacaacgcg gtcgcccgaa
    17761 cggcgggtaa gcgggtccag gggaacgtcg agcacttcgc ttgcggtcag gattttgggc
    17821 tttcgcttca gcgtgctgac gttgatctcc atgtcatcac ggatcacctt cgcctgacac
    17881 gcaagtcggt aatttccgga aaggaactgc tcggaatcgg ttctcggggt gagcgcctcg
    17941 attccctcgt gcacctggac tatgcactca tggcacgtgc ctacgcggtt gcaggagctg
    18001 gggactcgaa tcgcggggtt cgtgtctgcg tgttcgaaaa gtgttttgcc aacaacaatg
    18061 ggaagaacct ggtcattgaa acggcagttg ggcactggtg gactcctgag cgcgggacac
    18121 tgaacagcac ggctgttgtg caggggctgt ttgatgcggg gacatttccg tttccgaggt
    18181 gccgacgcct ggcaccgcgg tgaatacgat gaaatcaaag atagagcacg ccgccgaatc
    18241 cggccacccc cgccgctttc ctttgcccgt tgcggcaacc tgtgtgtacg ttgtattgaa
    18301 catttgccgg gttctcgtca gcacggtttc gattgggcat acgagcatgg aacagcttct
    18361 ctccccgctt cgatctatta acgtggtgct cgtgcgaccg gagtcgcccg gtaacgtcgg
    18421 ggcagttgcg cgagtgatac gcaatttcgg tctcggagag ttgcgtatcg ttggttctga
    18481 catagacacg gacgggccag ccgccagact tgcccaccgg tcgcttggcg tacttaaggg
    18541 tgcgcgtttc tacgaggatg tagactccgc ggttgctgac gccgagtgga taatcggcac
    18601 cagcgggaga ttgcgttccg gaagaccaat gccagcgacg gttcgctcgg cagcctcgcg
    18661 tatcgtggcg caatgttcac acggcgcggg tgcgatcctt ttcgggccgg aatcggacgg
    18721 gctttcatta cgtgacctcg cccattgcga cgaactcata cgcgttcccc agcgcaggga
    18781 tgctccggcg ctgaatctcg cgcaggcggt tgcggttgtc ggtgcggagc tctttcaggc
    18841 cgcactggta cccggcaacg cgcccgtgcc gccttcagtg acgagagagg cgcgcaaacg
    18901 atttgtgcgg cggctcaagg cgctgagtac tcattgcggc ctctccgtga gaaaccgccc
    18961 ggaggagact ctggaagagc tcgaaggaat atttgtcagg cggcgctttg agccacacga
    19021 aatggcggtt ctggagctat ggttgtcgca gcttgagtgg ttcaccggaa tggatcaggg
    19081 gtccaacggc agaggtacag cggagtgacc acaatgaggc gcagatcctc cttccttctg
    19141 gcatcgctcg ttttcaccgg tgcggccgcg tgcttcgcat cgcagtggca cgagatttcc
    19201 ctgcccgcga gttacgagcg tctgcattgc atggcgatgc tgacgccgga gtctggctgg
    19261 gcgggcggac agggcggtct gatacttcgg tgtgagggag ggcagtggag gaaagtcacg
    19321 gcgccgacac gaagcgacgt catagcgatg gcgttcgacg acgaagagaa cgggtgggtg
    19381 gccggctttg atgccgcaag ccagaagtct cttctatttc gttaccagag aggtcgctgg
    19441 acgcccatcc cggttcccgt ggggctgatc gtgactgcga ttgccagcga tggccgtgga
    19501 acacttttcg tgttcgggca gggcggacgc gcgtttcgac tccggcaacg cgtgtggctg
    19561 gaaacgggta cgcccgggta ccgaaccatg ctggccgccg tcagcctccc ggacggggct
    19621 gtctgggctg tcggcgaatt cggttccgca tggaggtgga acggatcaag ctggcagggg
    19681 actcagactc ctgtgcgctc acacctgcat g
//
LOCUS       DAOTNG010000014        18392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3066_length_18392_cov_5.620221, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000014 DAOTNG010000000
VERSION     DAOTNG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18392
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_18392_cov_5.620221"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..571
                     /gene="tnpA"
                     /locus_tag="P5179_01440"
     CDS             119..571
                     /gene="tnpA"
                     /locus_tag="P5179_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRS93918.1"
                     /translation="MPQSLARILVHVVFSTQNREPVLNSGVRNGLFPYCAAVLNENGC
                     SALAVGGVEDHMHLLFALGRTATVCKVVEMVKTSSSKWVKRQGEELAAFYWQGGYGAF
                     SVSPTHAQTVVRYIARQEIHHRTRSFQDEFRGLLRRYSLDFEEERVWE"
     gene            1267..2346
                     /locus_tag="P5179_01445"
     CDS             1267..2346
                     /locus_tag="P5179_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS93919.1"
                     /translation="MPAKAKSKPSKVRVGFVGSGGIARHYMGLFTKNMANDVEIVAIS
                     DSSDAALALAGDQFNIAKRFKNYKDMLKIKELDAVCVCTPNYLHAQPTIDALGAGKHV
                     IVEKPMAMNATEAAAMVKAGKDNKKILVIGFQYRYAPAAQVIKRAIDEGQFGKVLFAR
                     CQALRRRGIPNWGVFTRKDLQGGGPMIDIGVHIIEVAHYLMGSPKPVSAFGSTYTYIG
                     DKEEKAKGVACGWPNWDYKNYTVEDLAVGLIRFDNGATLSVEASFAAHIEKDVFSFTL
                     MGEKAGAQFEPLMLFKDEAGVMVNVTPTYVGGQDGFLTKLQDFIECVKTGKQPQAPGE
                     HGLMVQQILDGVYRSAETGKVVTFK"
     gene            complement(2869..3243)
                     /locus_tag="P5179_01450"
     CDS             complement(2869..3243)
                     /locus_tag="P5179_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS93920.1"
                     /translation="MKDVRTYLARIVECIDQIDAYTKGDRARFLQERIVQDAVIRNFE
                     VIGEAAKRVPEEFRAGFPEIPWRMMAAFRDVLIHDYESVSMPRVWNVVQNELPKVRSS
                     LASVLPPLDELEKELSSDGSPE"
     gene            complement(3240..3533)
                     /locus_tag="P5179_01455"
     CDS             complement(3240..3533)
                     /locus_tag="P5179_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS93921.1"
                     /translation="MDEIERLLREKRHEILRIAARHRARNVRVFGSIARGDARPDSDI
                     DLLVEFAPESDLFDQIALKRDVEELLQRKVDVVGDDSIYWLLHRRILKEAKQL"
     gene            complement(4190..7312)
                     /locus_tag="P5179_01460"
     CDS             complement(4190..7312)
                     /locus_tag="P5179_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS93922.1"
                     /translation="MNSPENRFPPVQHTGPLAWMVHNRVTATLLMLVFIIGGLVTAYR
                     IKQEVYPEFELDRVDIRVPYPGASPEEVEQGIILVVEEAIRGIDGIKQITATAAEGSG
                     SVSVEIEEGADAQRVYQDIQQAIQRISTFPQDAEKPQVALAISRRNVLAILIYGDVDD
                     WVLRDVAELVREKLLQKPGITQVDLSGVRDYEIHVDVPQENLRRYNLTLAQVAQTIRQ
                     VAVELPGGSIKSDAGEILLRVKERRDWAREFGDIPIITTPSGVVVHLSDIAEVREGFE
                     DSNRLATFDGQLAVNVDVYRVGDQTPIGVSNAAHEALEEIAPDFPPGVHYAFRMDQSV
                     TYRQRLDLLLRNAASGLILVLLVLGLFLAPKLALWVTMAIPTAFLGTILFMPALDVSI
                     NMMSMFAFIMALGLCVDSAIVAGENIYESRRRGMEVLDAAVQGVREVNLAIAFSMLTN
                     IASFVPLLFMPGWIGKIWRVVPFVVIFTTLIAWIQPILILPAQLARSLAAPEGSAMHH
                     VAVFQQKVSGALYRFINNVYDPFVEKALRRRYLTIAIFVAVLTIMVAYAANGRMGIVL
                     MAKVESDRAYVTATLPYGSPFQRAVAVRDRLVESAEKIRAEYGDALVQGIYANINENV
                     VEVAIYLTDPDIRPLSTGETTDIWREKTGQIPGLQSLKFESDRGGPGGGSSVTVELTH
                     SNIDVLAAASRDLARKLAHFTQTRDIDDGYSPGKQQLSFRVRPEGLSLGLTPTDVARQ
                     VRNSFLGTEALRQQRGRNEITVRVRLPEPERLSEYDVEQLLIRTPAGRDVPLYEVAEV
                     ERGRAFTSIARRDGRRTVTVRASVIPEKETNRVLESLQMEVLPQLVRDFPGLSWSFQG
                     RQATMTESMDSLYRGFLLALLVIYVLLAIPFRSYSQPLIVMTAIPFGIVGALLGHLIM
                     GYSIDLISMMGMVALAGVVVNNALVMIDYANRCRLEGDNAHDAIHRAGIRRFRPIILS
                     SLTTFCGLVPMIFETSRQARFMIPMAISLGYGILFATAITLILVPCLYLVIEDVKGLG
                     KGREEQETAQ"
     gene            complement(7309..8550)
                     /locus_tag="P5179_01465"
     CDS             complement(7309..8550)
                     /locus_tag="P5179_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS93923.1"
                     /translation="MADSSTPTPIPQKGGRRILRAAVPLAIVIAGSLVAFYLMKTAPR
                     AERRQRPVNARLVEVDTLHFAEHVVTIEAMGTVQPAQEVQVAAPVAGEVLNISPELIP
                     GGRFRKGQLLVQIDPADYDFAVERAAAEVTRARSELATEMGKQAVARREFELLGQQGG
                     EAERALALRQPQLETARAALASAEAALAQARLNRRRTSVFAPFNAIVKVAYVNAGARV
                     SANAPLAALMGTDEYWVTASVPVNQLRWINVPQSATGKGSPAVISNDVEWGSGVIREG
                     YVARVAGDLEEQGRMARVLISVPDPLAITRNNEGKPSLLVGAYVRVTIRGNPVPSAAK
                     IRRDLLREGDRVWIMNDSSRLEIRPVTIALKGPDVVYVTGGVREGERIVTTDLSAAVE
                     GQPLRTREEPAARNATRGTSR"
     gene            complement(8581..9993)
                     /locus_tag="P5179_01470"
     CDS             complement(8581..9993)
                     /locus_tag="P5179_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS93924.1"
                     /translation="MTGCGAKNSVIAYLVLPAALIAGCGPKPAGIAPPVAPPAEFSAA
                     GTQEAADRWWQSFGDAALNEAVERSLADNLSLRGAWFRLAQAEAAARREGAGAYPSMS
                     LSAGAELTRQHTGNVATNGTGTEFSLGAAASYEVDLWGRVRAASDASLLDARASAEDV
                     RTAAVTLSAQVATTWFRISGQEAETAVLRRQLRSNEEMLELVTLRFQQGQSKAEDVLR
                     QRQAVEARRGDIARGEANRAVLCNRLAVLLGQNPGTSAFRPPPDLPDLPPMPAAGVPA
                     DLIRRRPDVHKAYFEVMAADRRAAAAIADRFPRITLSAGANTSAESVTDLFSNWLASL
                     AANVAMPLVDGGRRKAEVERTRAATSASLTAYGQTVLDALAEVENAIATERRQQEYVT
                     SLRQQLSLAAEVINRARDNYLQGVTEYTRVLEAQQTHQALEISYLGAQLALIENRIAL
                     YRALSGGWELTRPGPLTGSR"
     gene            complement(10023..10466)
                     /locus_tag="P5179_01475"
     CDS             complement(10023..10466)
                     /locus_tag="P5179_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRS93925.1"
                     /translation="MARTVLVVYHSMGGNTAAAAEIVAEGIRSVAGADAVVKTAAEAG
                     PSDLLACDGVCFGSPDYFSYMAGMLKDFFDRTFYPTQGQVDDKPCAIFITHGGGGSAS
                     TSVEKICRSFRFKQVGQTLLVRGKPDDAARTKLRDLGALLAKTVL"
     gene            complement(10564..10743)
                     /locus_tag="P5179_01480"
     CDS             complement(10564..10743)
                     /locus_tag="P5179_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93926.1"
                     /translation="MPQTTEGGLRKGKRHIKTTPPGSDVEDAGFSAGLLKQGPGKPLW
                     ILSPLKGPMRTAAKW"
     gene            10748..12316
                     /locus_tag="P5179_01485"
     CDS             10748..12316
                     /locus_tag="P5179_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93927.1"
                     /translation="METPFRVLEIGTTPQLLIDDYVVEDVWMIRRSPEEPVKHLGNPI
                     FPAGDTVIYDDEEKLFKMWYGIGIPGTFEAKGAYAVSSDGLHWEKPELGLVEADGSTR
                     NNLIETTGFGSVLKDPRDPDPARRYKMMTKRAGTPESEGRAFAAFSPDGIHWKDHPGE
                     KSILRASNDGNGMVLRDDSTGKYINFRRTTVLAGGRGVLPEDIGFPDPRVMKGAGGDR
                     LGVPEGIGFPSEDDFESYEEAEEYIHRYLRPAPYVHTRTLRIFRDTHEGLGCNRRIAR
                     SESDDFLHWTEPEVIIRPDELDPPRLYSISVARYLGMYIGLLELYDSCGNRRAPGTPQ
                     ESDTIDTQLVFSRDGWKWERLANRPVFLRRGIIGAFDCGMLRPVTPPFMVLGDEIRIY
                     YYGHGSSHNIPSPNQGLGVARLPKERFVARVAGDEVGALITKPFRLEGGELQINANAA
                     KGMIKVEVTDPFGNPLEGFSAKEAVEIRENGFRVPVQWTCGKRLSEIAGRVVRLRFYL
                     YRSRLYSFVVDEGR"
     assembly_gap    12342..12441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12442..13192)
                     /locus_tag="P5179_01490"
     CDS             complement(<12442..13192)
                     /locus_tag="P5179_01490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS93928.1"
                     /translation="MEKGEKVALVTGASRGIGRGCAEALAADGWSVVVNYRSHEDEAR
                     AVAERCRGFGVRAVTAMADVRDREAVDAMFARTVEHFGGLHLFVSNAADSVRKPFVDL
                     SVEEIAHTLDVSLWGVIHTAQAAARLLVRQNTGGAMVFVSSVHVPFPYANCADYNIAK
                     AGGHALAMTLAAELAKHRIRVNIVEPGWVDTPGERKWSSEEDIAREGAKLPWGRLATI
                     EEIGQAVAFLASEKAEYITGSVLKVDGGFTLP"
     gene            complement(13286..13483)
                     /gene="rpmB"
                     /locus_tag="P5179_01495"
     CDS             complement(13286..13483)
                     /gene="rpmB"
                     /locus_tag="P5179_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRS93929.1"
                     /translation="MAYQCELCGKSFQRGNTVSHANNRRKRLFKANLQRIHVMVGSTS
                     KHVRVCTQCIKSGRVEKKTAA"
     gene            13677..13973
                     /locus_tag="P5179_01500"
     CDS             13677..13973
                     /locus_tag="P5179_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93930.1"
                     /translation="MHTWGVAVLLLVVTWVVLAGLPGAPVQSLAGKLILAIGFFVIGV
                     GFYLLSIGPVDSVQSMTWAPLALVAGFCVIVPLGLLYHRRAPGSEEDAATKTRL"
     gene            13979..14052
                     /locus_tag="P5179_01505"
     tRNA            13979..14052
                     /locus_tag="P5179_01505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14013..14015,aa:Val,seq:tac)
     gene            complement(14105..14575)
                     /locus_tag="P5179_01510"
     CDS             complement(14105..14575)
                     /locus_tag="P5179_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93931.1"
                     /translation="MKKPQIPLSENHRRIVASSLRYAEDQILFCRRLLTETSENRMTR
                     FADEYSDDQIRRLVEMCDELHEKLVSFADRAGVSPAERSVSRHVSGTMSSVWETLEDL
                     RPRKLKAYGEVPADAAREIEPIVEDAVQIIERVLEFSRRCTSRSDSGAGARQPE"
     gene            complement(14572..16413)
                     /locus_tag="P5179_01515"
     CDS             complement(14572..16413)
                     /locus_tag="P5179_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRS93932.1"
                     /translation="MCIPETDVRAPQTAGGENGDITNANGLGELFDEALSILGTAGPE
                     FKTFSDAIAELKTRLAEERFHLAVLGQFKRGKSTLLNALLGEAILPSSVVPLTAIPTF
                     VRAGETRRALVYFDNGVPPRAFEADDAGAIREFIAQFVTESGNPKNERGVTQVDVFSS
                     APILRQGVVLIDTPGIGSTHRHNTEATLNFLPQCDAAMIVVSADPPITEVEVEFVRQV
                     KRRINRLFVVLNKIDYHTEADVREATGFLKSVLADKLGFSDDHPIFPVSAHLGLNARQ
                     NSDADLWERSGMHRVERHLSRFLASEKKAALSEAVRHKAADTVEEALTQFRLILRSLQ
                     LPAEELDKKRAFFRQKLDEIERNRLVADDLLKGDHRRTRARLEEYIDAVRAQARTGFR
                     GIIAGALARNADTQQAESDARDALAEAIPVFFEQKVGQTSAFLGEEIDAVLTPHQSRA
                     EELVESVRKAAASIFEVPYQPAGKVITFDIVQKPYWIARTWSTSLSPIPEGFWDRFLP
                     ERVRRHRLQTRLNNQLEDLVISNAENLRWAMLRTVDETFRHFRSTLVTRLGETITATN
                     GALNSATEKRGLHEEQTASLIHGIQETIRQLENVEHSLRPPPTGAAP"
     regulatory      complement(16436..16500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16569..17786)
                     /locus_tag="P5179_01520"
     CDS             complement(16569..17786)
                     /locus_tag="P5179_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS93933.1"
                     /translation="MAAIRNAIDFLGLKRSIIGVLSMVVLVGMGERMAERFLPIYLFA
                     LGGGVLSVGLLNGLDNLLSALYSFPGGYLADRIGTKRALIVFNLMAMTGYCVVIFIPV
                     WQAVIIGAFFFLSWTAISLPATMGLVAVVLPKNKRTMGVSMHSLVRRIPMALGPLVGG
                     AFISIWGEREGVRAAFIAAFIFAAVAMVMQQLLIEDKREEASAPLSGNPFRLIGLMNA
                     DLRNLLVSDILVRFCEQIPYAFVVIWCMKVIANPVSAIEFGVLTTIEMATAVLCYIPV
                     AYFADKSGKKPFVVITFVNFTLFPVALLFSHSLLPLVGAFILRGLKEFGEPTRKALIM
                     DLAPEGAKAGMFGLYYLLRDSVVSLAAFGGAFLWMISPAVNLISASAFGLAGTLWFLW
                     RGRDMPVLSTATA"
     gene            complement(17943..>18392)
                     /locus_tag="P5179_01525"
     CDS             complement(17943..>18392)
                     /locus_tag="P5179_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS93934.1"
                     /translation="LRTLTVGFLTPFYFLRAGSLVALPALIAAPAVFIVFLAAKTVSK
                     IFGLLPVVSAFRTEKNERWYYTLMMSTGLTFGTISALYGFSHGIVTQSQYSFLVATVI
                     ASAVIPTLIANAAFMPRHLLPETAPVSVEPAGRRALPPKEEEDVGEE"
BASE COUNT         3479 a         5595 c         5598 g         3620 t
ORIGIN      
        1 gacccgggct gtgctgtttc gccccgttgg ggctgcctgc gcgtttcttg accgctcagg
       61 atggttccta tcagggcgtg gttcgcctgt agcatctatc ggtacgcgag gtattgccat
      121 gccccagtct ttggcacgta tcctagttca cgtcgtgttc agcacccaga accgggagcc
      181 ggttctgaac tcgggcgtcc gcaacgggtt gtttccgtat tgcgcggcgg tcctcaacga
      241 aaatggctgc tccgctctgg cggtcggcgg cgtggaagac cacatgcacc tgttgttcgc
      301 tctggggcgc accgcgacgg tttgcaaagt agtcgaaatg gtcaagacca gttcgtcaaa
      361 atgggtgaaa aggcaggggg aggaacttgc ggctttctac tggcaaggcg gatacggcgc
      421 attctcagtg agtccgacac acgcgcaaac ggttgtccgg tacatcgcgc ggcaagagat
      481 ccaccaccgc acgcggagtt tccaagatga atttcgtggc ctcttacggc ggtactcgtt
      541 ggactttgaa gaagagcgcg tgtgggaatg atcccgccgt ccggcccggc aaggcctgaa
      601 ggaccgccat acgttagccc gcgggcagcg ccccgggcgg ggaaggcctc gcccgtgctg
      661 cggccttgag attgccggcg aatcgcggag aagatctgat ttacgccgcc cgggcctccg
      721 gacccgggct atgctgtttc gccccgttgg ggctgcctgc gcgtttcttg accgctcagg
      781 atgggtcctc tcagcgcaag cgccatggtg aggcctgaag ggccgacata cgccagcccg
      841 cgggcaacgc cccgggcggg gaaggccccg cctgtgttac ggccttgagg ttcaggcggg
      901 gcgcggagaa gatcggaatt acgtcgcccg ggcctgcgac ccgggctgtg ctgtttcgcc
      961 ccgttggggc tgcctgcgcg tttcttgacc gcgcaggcgg catgttacag caccaagcgc
     1021 gacgcctgaa cgtgtggaaa cgcgcgcgag gctccccgat tcagcgaggc cggggcttcg
     1081 acaagctcag ccaccggcgc gctgccgggc ggcttgtcgt cgcaactcga aacacggtcc
     1141 gcctcccccc tcggcttttc cccacgggaa acccttgtgt atccttatta gagcatttgc
     1201 gcgtcttttt ccgcccgttt ttcgggctga catcatcctt tcactactgc ttaccaagga
     1261 gccatcatgc cagcgaaggc caaaagcaaa cccagcaagg tgcgtgtcgg atttgtcggt
     1321 tccggcggca tcgcccgtca ctacatgggc ctgttcacga agaacatggc gaatgacgtc
     1381 gagatagtcg ccatctccga ttcgagcgac gccgcgcttg cgctcgcggg cgaccagttc
     1441 aacatcgcca agcggttcaa gaactacaaa gacatgctca aaatcaaaga gctggacgcg
     1501 gtgtgcgtct gcaccccgaa ctacctgcac gcgcagccga cgattgacgc gctgggggcg
     1561 gggaagcacg tcattgtgga aaaaccgatg gcgatgaacg ccaccgaggc cgccgccatg
     1621 gtgaaggccg gcaaggataa caagaagatt cttgtgattg ggttccagta ccgctacgca
     1681 ccggccgcgc aggtgatcaa acgcgcaatt gacgaggggc agttcggcaa ggtgctgttc
     1741 gcgcggtgtc aggcgctgcg ccgccgtggg attcccaact ggggcgtgtt cacccggaaa
     1801 gaccttcagg gcggcggccc catgattgat atcggcgtcc acatcatcga ggtcgcgcac
     1861 tacctcatgg gaagcccgaa acccgtatcg gccttcggct cgacgtacac ctacatcggc
     1921 gacaaagaag agaaggccaa gggcgttgcc tgcgggtggc ccaactggga ttacaagaac
     1981 tacacggtgg aagacctcgc cgtggggctg attcgtttcg acaacggcgc gactctttcc
     2041 gtggaggcct ccttcgcggc gcatattgag aaggatgtgt tcagcttcac cctgatgggc
     2101 gagaaggctg gggcgcaatt cgagcctctg atgctgttca aggacgaggc cggcgtgatg
     2161 gtgaacgtca cccccacgta tgtgggtgga caggatggct tcctcaccaa gctgcaggat
     2221 ttcattgagt gcgtgaaaac cgggaagcag ccgcaggcac cgggcgagca cgggctcatg
     2281 gtgcagcaga ttctcgacgg ggtgtaccgc tccgcagaaa cggggaaggt tgtgacgttc
     2341 aagtagcgcg cgcgtcacag cgcgagtgcg agcaggaaac cgcacgtgcc gtcatggtac
     2401 gtgcggtttt gcgttggaaa cagatgcgcc gcttggaaaa cgaaccagga agagggatga
     2461 gcaaccgtca ttcccgcgaa agcgggaatc caatacactc gccggtgcgt ctcgatacaa
     2521 cgaacgcacg gcgttcgcca ctcgacgacc ggcgggctct gggatcgagc aggttcgcga
     2581 caggaaggaa tgcctgcccc gccatcattt cctcgtcgtc tggtggggcg gactgcccat
     2641 accggggcat gccaccgccg cccggtcctc cgaaccgggc tggattattg cgcgccgttg
     2701 gcgcttcttg ccaacgacta ttccgcgggt ttcccatcgt tgtggctgcc tcccaagaaa
     2761 aaggcgacat tcccgcgcag ggggcaatcc aacgcaacgc tgtggaggcc tcggcgagac
     2821 cgccctgccc gcgggtcggg aacacacggg gcgcttgtcc agcctgcctt actccggcga
     2881 cccgtcgctg gagagttcct tttccagctc gtccaaaggc ggcaggacgc tggcaaggga
     2941 agacctcact ttcggcagct cgttctgtac tacgttccag actcgtggca tgctcaccga
     3001 ttcgtaatca tgtatcagaa cgtcgcgaaa ggccgccatc atgcgccacg gaatttcagg
     3061 gaacccggcg cggaactcct ccggaacgcg cttcgcagct tcgccaatga cctcgaaatt
     3121 gcgaatgaca gcatcctgca caattcgctc ttgaaggaaa cgcgctctgt cccccttcgt
     3181 gtacgcatct atctggtcga tgcattccac gattctcgcc agatatgtcc gcacatcctt
     3241 cacaactgct tcgcctcttt gagtatgcgt cggtggagca gccagtagat actgtcatcc
     3301 ccgaccacgt ccactttccg ctgaagcaat tcttccacat cccgtttcaa ggcgatctga
     3361 tcgaacaggt cgctttcagg tgcaaattcc actagcaggt cgatgtcgct gtcgggccgg
     3421 gcgtctccac gcgcaattga accgaacacg cgaacatttc gtgcgcgatg cctcgcggcg
     3481 atccgcagga tctcatgccg tttttcgcga agtagccgtt cgatctcgtc catgattcgg
     3541 gcctttgata agtttcatca aatatactaa acggcacagc acctgccttc ggtttcggag
     3601 gcggcacggg taagacttga aaacgccacg gcttttgccg catcctgcga ggaagcgctg
     3661 cctgcgcccg cagacgagcg caacaccgag acaattcggg agcttgcgcc cagcgcgcgg
     3721 ttctggcgtg acgggcggga accgaccgtt gtatcgagag gccgcgccgg tgcgtctcga
     3781 tacagcgaac gcgcggcgtt cgccactcga cgaccggcgg gctctgggat cgtgcaggtt
     3841 cgcgacaggc aggaatgcct gccccgccat catttcctcg tcgtctggtg gggcggactg
     3901 cccataccgg ggcatgccac cgccgcccgg tcctccgaac cgggctggat tattgcgcgc
     3961 cgttggcgct tcttgccaac gactattccg cgggtttccc atcgttgtgg ctgcctccca
     4021 agaaaaaggc gacattcccg cgcagggggc aatccaacgc aacgctgtgg aggcctcggc
     4081 gagaccgcgc cggtgcgtct cgatacaggg aatgcacggc gttcgccgct cgacgaccgg
     4141 cggggtgatg cagggcaaag aacggtgcag gatcgccacc tgcgcccgtt tactgagccg
     4201 tttcctgctc ctcccgtccc ttccccagcc ccttcacatc ctcgatgacg aggtagagac
     4261 agggcacgag gataagcgtg atcgcggtgg cgaagaggat tccgtagcca agggagattg
     4321 ccatcggaat catgaaacgc gcctggcgcg aggtttcgaa aatcatcggc accagaccgc
     4381 agaacgtggt aagcgaggaa aggatgatcg ggcggaagcg ccggattccc gcgcggtgga
     4441 tcgcgtcgtg cgcgttgtcg ccctccaacc ggcaccggtt ggcgtaatca atcatgacga
     4501 gggcattgtt cacgacgaca cccgcgagcg caaccatgcc catcatgctg atgaggtcaa
     4561 tcgaatagcc cattatcagg tggccaagca gtgcgccgac gatgccgaac gggatcgccg
     4621 tcatgacgat gagcggctgg ctgtagctcc ggaacggaat ggcaaggaga acgtagatga
     4681 caagaagcgc cagcaggaat ccccggtaga ggctgtccat ggactccgtc atcgttgcct
     4741 gacgcccctg gaaactccag gagagccctg ggaaatcgcg caccagttgc ggcagaactt
     4801 ccatctggag cgattccagc acgcgattgg tttccttctc ggggatcacg ctcgcccgca
     4861 ccgtaacggt gcgccgccca tcgcgccgcg cgatgctggt aaaagcccgc ccccgctcca
     4921 cctcggcgac ttcatagagc gggacatcgc gaccggccgg cgttcgaatc agaagctgct
     4981 ccacgtcgta ctcgctcagg cgctccggtt ccgggagccg gacgcggacg gtgatctcgt
     5041 tgcgcccccg ctgttgccgc agcgcttccg tcccgaggaa cgaattgcgc acctgccgcg
     5101 cgacgtccgt aggcgtcaac ccgaggctca acccttccgg tctgacgcgg aagctgagct
     5161 gttgcttccc ggggctgtac ccgtcgtcaa tgtccctggt ctgcgtgaaa tgggcaagtt
     5221 tccgcgccag gtcgcggctg gccgccgcca gaacatcgat gttggagtgc gtcagttcca
     5281 cggttaccga ggaacctcct cccgggccac cgcggtccga ctcgaatttc aatgactgaa
     5341 gaccgggtat ctggccggtt ttttcccgcc agatgtccgt tgtctcaccc gtggaaaggg
     5401 gacggatatc ggggtcggtc aggtagatgg ccacttcgac gacgttttcg ttgatattcg
     5461 cgtaaatccc ctgcacgagc gcgtcgccgt attccgcacg gatcttctcc gcgctttcca
     5521 cgagccggtc gcgtacggca accgcgcgct ggaaagggct tccgtaggga agcgttgccg
     5581 tgacgtacgc gcggtcggat tcaaccttcg ccatgaggac aattcccatc cgtccattgg
     5641 cagcgtatgc aaccattatt gtcaacacag ccacgaatat ggcgatagtc aggtatctcc
     5701 ggcggagagc cttttcgacg aacgggtcgt agacgttgtt gatgaaccgg tacagcgcac
     5761 cgctgacttt ttgctgaaac acagccacgt ggtgcatggc gcttccctcg ggcgcggcga
     5821 gagagcgcgc gagctgggcg ggaaggatca gaatcggttg aatccacgcg atcagcgttg
     5881 tgaagatgac cacgaacggt acaactctcc agatttttcc gatccagccc ggcatgaaca
     5941 ggaggggaac gaatgacgcg atgttcgtga gcatgctgaa cgcaatggcc aggttgactt
     6001 ctcgcacgcc ctgcactgcc gcatcgagta cctccatacc ccggcggcgg gactcgtaga
     6061 tgttttcccc ggcaacgatc gcactatcca cgcacaggcc gagcgccatg ataaaggcga
     6121 acatgctcat catgttgatg gaaacgtcca gcgccggcat gaacaggatg gttccaagaa
     6181 acgcggtggg gatggccatc gtaacccaca gcgcgagctt cggtgcgagg aaaaggccga
     6241 gcaccagcaa cacgagaatg agaccggacg cggcatttcg aagaagcagg tcgaggcgct
     6301 gacgatacgt gacactctgg tccatgcgaa atgcatagtg cacgccggga gggaaatccg
     6361 gcgcaatttc ctcaagcgct tcatgcgccg cgttggacac gccgatgggg gtctgatcgc
     6421 ccacgcgata gacgtccaca ttgacagcca gttgcccgtc gaatgtcgcc aggcgattgc
     6481 tgtcctcgaa cccttcgcgc acctcggcga tatcgctcag atgcacgacg acgccggagg
     6541 gtgtggtgat gatcgggatg tctccgaatt cgcgggccca gtcgcgcctc tccttgacgc
     6601 gaaggaggat ctctcccgcg tcgctcttga tgcttccccc gggcaactcg accgctacct
     6661 gccggatggt ctgggcgacc tgcgccagcg tgaggttgta ccgccgaagg ttttcctgcg
     6721 gaacatccac gtggatttcg taatcccgca ccccgctgag gtcaacctgg gtaatccccg
     6781 gtttctgcag cagcttctcg cgcacgagct cggcaacatc acgcagaacc cagtcgtcaa
     6841 catctccgta gataagaatc gccaggacgt tccgccgcga gattgccagc gctacctggg
     6901 gtttttctgc gtcctgggga aacgtggata tccgctgtat tgcctgctgg atatcttggt
     6961 acacgcgctg ggcatccgcg ccttcttcga tttccaccga caccgagccg gagccttcgg
     7021 cagccgttgc cgtgatctgt tttatcccgt ctatcccgcg gattgcctcc tcaacgacga
     7081 ggatgatgcc ctgttccacc tcctccgggc tcgcaccggg atagggcacc cggatgtcaa
     7141 cccggtcgag ttcgaactcc gggtacactt cctgcttgat ccggtacgca gtgacaagcc
     7201 ccccgattat gaacacgagc ataagcagag ttgcggtgac tcgattgtgc accatccacg
     7261 caaggggtcc ggtgtgctgc accggcggga acctgttctc cgggctgttc accggctcgt
     7321 ccccctcgtg gcgttgcgcg ctgccggttc ctcgcgtgtg cggagcggtt ggccttccac
     7381 cgcggcgctg aggtcggtgg ttacgatgcg ttccccttcc cgcacgccgc cggtgacata
     7441 gacaacatcc gggcctttca gggcgatggt taccggtcga atctcgaggc gactgctgtc
     7501 attcatgatc cacacgcggt cgccttcgcg aaggaggtcc cggcggattt tggcggccga
     7561 gggcaccgga ttgccgcgta tggtaacgcg cacataagcg cctaccagca gggagggttt
     7621 gccctcgttg ttccgggtga tcgcaagcgg atcgggcacg gagatgagca cgcgcgccat
     7681 gcgcccctgt tcttccagat cgccggccac ccgtgccacg tatccttccc ggatcacccc
     7741 gctgccccat tcgacatcgt tggagatgac tgccggcgag cccttccctg tcgcggattg
     7801 cgggacgttg atccaccgca gctggttcac cggcacagac gccgtcaccc agtactcatc
     7861 cgttcccatg agcgcagcca gtggcgcgtt ggcggaaact cgcgctccgg cgttgacata
     7921 cgccaccttg acgattgcat tgaacggagc aaagacactg gtgcggcgtc gattcaaccg
     7981 cgcctgcgcc agtgccgctt ccgctgacgc gagcgccgcc cgcgccgttt caagctgcgg
     8041 ctgacgaagc gcaagagctc tctccgcctc cccgccctgc tgccccagaa gctcaaactc
     8101 gcgccgcgcg accgcctgtt tccccatctc ggttgcaagt tcgctgcgcg cgcgggtcac
     8161 ctccgccgcg gcgcgttcaa ctgcaaaatc gtagtccgcg gggtcaatct ggacgaggag
     8221 ttgccccttg cggaatcggc cgcccggaat gagttcgggg ctgatattca gaacctcgcc
     8281 ggccaccggc gccgcaacct gtacctcctg cgcgggttga accgttccca ttgcttcaat
     8341 ggtgaccacg tgttccgcga aatggagggt atctacctcg acgaggcggg cgttaaccgg
     8401 ccgctggcgc cgttccgccc tcggggctgt tttcatgaga tagaacgcga cgagcgaccc
     8461 cgcgatcacg atcgccagtg gaaccgccgc ccgcagaatc ctcctgccac ctttctgcgg
     8521 gatgggtgtg ggtgtgctgc tatctgccat tttgttccgt ttcctgcgaa aaggctttgg
     8581 tcaccgcgag cctgtgagtg gtccggggcg ggtcagctcc caaccgccgc tgagggctct
     8641 gtagagggcg atgcggttct cgatgagcgc aagctgcgcg cccaggtagg agatttcgag
     8701 cgcctgatgt gtctgctggg cttcaagcac gcgagtgtat tcagtaacgc cctgcaggta
     8761 attgtcccgg gcacgattga tcacctctgc cgcgagggaa agctgctgtc ggaggctggt
     8821 cacgtattcc tgctgccggc gttccgtggc gatggcgttt tcgacctcgg cgagcgcatc
     8881 gagcacggtc tgcccgtacg cggtaaggct tgccgatgtt gcggcccgcg ttcgctccac
     8941 ttccgctttg cgccttccac cgtccaccag aggcatggcg acatttgccg cgagagaggc
     9001 aagccagttc gaaaacaggt ccgtgacgga ttccgcagag gtgttcgctc cggcggagag
     9061 ggtaatccgg ggaaaacggt cggcaatcgc ggcggcggct cggcggtctg cagccatcac
     9121 ctcaaaatat gctttgtgga cgtccggccg ccgccggatg aggtccgccg gcacacccgc
     9181 cgcaggcatc ggcggaaggt ctggaagatc ggggggtgga cggaaggcac tcgtgccagg
     9241 gttctggccc agcagaacgg caaggcggtt gcaaaggacg gccctgtttg cctcccctcg
     9301 cgcaatgtcg ccgcgacgcg cttcaacggc ctgtcgctgg cgtaatacat cctccgcttt
     9361 tgattgcccc tgctggaacc ggagggtgac cagctcaagc atttcctcgt tcgatcgaag
     9421 ctggcgacgc aggactgcgg tttccgcctc ctgtccggag atccggaacc atgtggtggc
     9481 tacctgagcc gaaagggtaa cggcagccgt gcgaacgtct tccgcggacg ccctcgcatc
     9541 cagaagcgag gcgtcggaag ccgcacgcac gcgcccccag agatccacct cgtaactcgc
     9601 ggcggcgccg agagagaatt ccgtgcccgt gccgttcgtg gcgacgttcc cggtgtgctg
     9661 tcgcgtcagt tcggccccgg cggaaagaga catgctcggg taggcacccg cgccctcacg
     9721 ccgcgccgcg gcttccgcct gcgcaagacg aaaccacgcg ccccgaagag aaagattgtc
     9781 tgcgagggag cgctcaacgg cttcgttcag cgccgcgtct ccgaaactct gccaccaccg
     9841 atccgccgct tcctgcgttc ccgcggcgga aaactcagcc gggggagcga ccggtggagc
     9901 aatccccgcg ggctttggtc cacaccctgc aatcaacgcg gctggcaaca cgagatacgc
     9961 gattacgctg ttttttgcgc cacaaccagt cactcgttac ctttctgcgc attcacgaca
    10021 catcacagaa ctgttttcgc cagcaatgcg ccgagatcgc gcagttttgt tcgtgccgca
    10081 tcgtccggct tgccgcgcac aagcagcgtc tggccgacct gtttgaacct gaacgagcgg
    10141 cagatctttt ccacacttgt cgaggctgac ccgccgcccc cgtgcgtgat gaagatggca
    10201 cagggcttat cgtccacctg cccctgcgtc gggtagaacg tgcggtcgaa gaaatccttg
    10261 agcatcccgg ccatgtagct gaagtagtcg ggtgagccga aacagacacc gtcgcaggcc
    10321 agcagatcag atggaccggc ttcggcggca gtttttacga ctgcgtccgc accggcaacg
    10381 gatcggatcc cctcggccac aatttctgcg gcagccgccg tgttgccgcc cattgagtgg
    10441 tacaccacga gcacggttct tgccatggaa cacctccctg tggtggactg tgaagagcca
    10501 gtgtatgtgg gaaatttaca cactgcggtg tatcggggcc aaaaggcaag cacgactcac
    10561 ggctcaccat ttcgcagcag tccgcatggg gcctttcaat gggctcagta tccacaacgg
    10621 ctttccgggg ccctgcttca gaagtccggc actgaaaccc gcgtcttcca catccgagcc
    10681 cggtggcgta gttttgatgt ggcgcttccc ttttcgcagt cctccctctg tagtttgggg
    10741 cataccgatg gaaactccgt ttcgagtcct tgaaatcggc acgacgccgc agttgcttat
    10801 tgacgactac gtggtagagg acgtctggat gatccggcgg tcgcccgagg aacccgtgaa
    10861 gcacctcggc aaccccattt ttcccgcggg tgatacggtc atctacgacg acgaagaaaa
    10921 gctcttcaag atgtggtacg gcatcggcat ccccggaacc ttcgaggcga agggggccta
    10981 cgctgtttcg tccgacgggc tgcactggga gaaaccggag ctgggactgg ttgaggccga
    11041 tggatccacg cggaacaacc tcattgaaac aacggggttc gggtcggtat tgaaggaccc
    11101 gcgcgaccct gatcctgccc gccgttacaa gatgatgacg aagcgcgcgg gaacgcctga
    11161 gtcggaaggc agggctttcg ccgcattctc gcccgacgga attcactgga aagaccaccc
    11221 gggcgagaaa tccattctcc gggcatccaa tgacggcaac gggatggtgc ttcgggatga
    11281 ctccaccggc aagtacatca actttcgccg cacgacggtt ctggcgggtg gaaggggagt
    11341 actgccggag gatatcgggt tccctgatcc ccgggtaatg aagggcgcgg gcggggacag
    11401 gctcggggtt cccgagggca tcgggtttcc ttcggaggat gacttcgagt cgtatgagga
    11461 ggcggaggag tatatccacc gctacctgcg gccggcaccc tacgtgcata cgcggacgct
    11521 gcgcattttc cgggacacgc atgaagggct cgggtgcaat cgccggatcg cgcggtcgga
    11581 aagcgacgac ttcctccact ggaccgaacc tgaagtcatc atacgtcctg acgagcttga
    11641 cccgccacgg ctctactcga taagcgtcgc caggtatctc gggatgtaca tcgggcttct
    11701 ggagctgtac gattcgtgcg ggaaccggcg cgcgccgggc actcctcagg aatccgacac
    11761 catcgatacc cagctcgtat tcagccgcga cgggtggaag tgggaacgcc ttgcgaaccg
    11821 gccagtgttc ctccgccggg gcattattgg cgcgttcgac tgtgggatgc tacgtccggt
    11881 cacccccccg ttcatggtgc tgggcgacga gattcggatc tactattacg gccacgggag
    11941 cagccacaat attccgtcgc caaatcaggg cctgggcgtc gcgcgactgc cgaaggagcg
    12001 attcgtcgcg cgcgtggcgg gagatgaggt cggtgcgctc atcacgaaac cgttccggct
    12061 ggagggcgga gaactgcaga tcaacgcgaa cgccgcgaaa gggatgatca aggtcgaggt
    12121 gacggacccc ttcgggaatc cgctggaagg gttcagcgcg aaggaggccg tggaaatacg
    12181 ggaaaacggg ttccgcgtgc ccgtgcaatg gacgtgcggg aaacggcttt cagaaatcgc
    12241 cgggcgggtc gttcggttgc ggttctatct ctaccgatcg agattgtact cgttcgtggt
    12301 ggacgaaggg aggtaggttt ccacagaggg gcatgcggac gnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn ntcggcagcg tgaagccgcc gtccactttg agcacggaac
    12481 cggtaatgta ttccgctttc tccgaggcaa gaaacgccac cgcctgcccg atttcctcaa
    12541 tggtggcaag gcggccccag ggcagcttcg cgccttcgcg ggcgatatcc tcttccgaac
    12601 tccacttccg ctcgcccggc gtatctaccc agcccggctc gacgatattg acccggatgc
    12661 gatgcttcgc gagttcagcg gcgagagtca tcgcaagggc gtgtccgcct gccttggcaa
    12721 tgttgtaatc cgcgcagttg gcgtacggga acggcacatg aacggagctg acgaacacca
    12781 tcgcgccgcc ggtgttctgc cggaccaaaa gccttgctgc cgcctgcgcc gtgtgaatga
    12841 cgccccacag gctcacgtcg agcgtgtgag cgatttcctc gacgctcaga tcaacaaacg
    12901 gtttgcgcac actgtccgcc gcgttggaaa cgaagaggtg gaggccgccg aagtgctcca
    12961 ccgtgcgggc gaacatggca tccaccgcct cccggtccct gacatccgcc attgcggtga
    13021 ctgcccgaac gccgaaaccg cggcaccgct ccgccactgc tctggcctcg tcctcgtggg
    13081 aacggtaatt gacgacgaca ctccacccgt ccgcggcgag cgcttcggca cacccgcgtc
    13141 cgatgccgcg tgaagcgccc gtcaccagcg cgaccttttc gcctttttcc atcgttttcc
    13201 ctcccgaagg ggaacagaag ccacaggaat gaaacaagcc ccgcatcctt cggacgcggg
    13261 gctttgaaac ggtctgggcg gggtatcagg cggcggtttt cttttccacg cgcccgcttt
    13321 tgatgcattg cgtgcacaca cgcacgtgct tggaagtgct gccgaccatc acatggatgc
    13381 gctggaggtt cgccttgaag agccgtttgc gccggttgtt ggcgtggctg accgtattac
    13441 cgcgttggaa gcttttgccg caaagttcgc attgataagc catgagtgaa atcctcggtt
    13501 cggcacggag atcgggtttt cagaaagatt ttaatgatag aacaggaaac gccttttgtc
    13561 aagtcccggg cgagccgggt cgcgcggtcg gcacgcacga aggttgacct tgcgcgggcc
    13621 tggcgctacc tttttcttgc gtttttgtcg gtccgcctcc tcgcggggag agattcatgc
    13681 atacctgggg tgttgccgtt ctacttctgg tggtgacgtg ggtggtcctt gccgggctgc
    13741 ctggcgcgcc ggttcaaagc ctcgcgggga aactgatcct tgccatcggt tttttcgtga
    13801 tcggcgtcgg tttctacctc ctcagtatcg ggccggttga cagcgtccaa tccatgacgt
    13861 gggcgcccct tgccctcgta gccggcttct gtgtgatcgt tccgctgggg ctcctctatc
    13921 accgcagggc gccaggttca gaagaagacg cagcaacgaa aacccgcctg taaatccggg
    13981 gcgcttagct cagttggtta gagcgtctgc cttacaagca gaaggtcgct ggttcgaatc
    14041 cagcagtgcc cattaccttt gccagattcg cccgcgcggt cgcgccttac cagcatgagt
    14101 cgcgttattc aggttgtcgg gctccggcgc ctgaatccga ccgggaagtg caacgtcgtg
    14161 agaattccag cactcgttcg atgatctgga cggcatcttc gacgatcggt tcaatttccc
    14221 tggctgcatc agccggaacc tctccgtagg ctttcagctt tcggggccgg agatcttcca
    14281 gcgtttccca gacgctggac attgttcccg agacatggcg actgaccgaa cgctctgcgg
    14341 gagacactcc cgcgcggtcg gcaaaactga caagtttttc gtgcagttcg tcgcacatct
    14401 ccaccagcct gcgaatctgg tcatccgagt actcatcggc gaaacgtgtc atccggtttt
    14461 cggaggtctc agtcagcagc ctcctgcaga agagaatctg gtcttctgca taccggagtg
    14521 aggaagccac tattctccgg tgattttcgg agagaggaat ctgcggtttt ttcatggtgc
    14581 ggctcccgtg gggggcggac gcaacgagtg ttccacattt tcaagctgcc ggatcgtttc
    14641 ctgaattccg tgaatgagag atgcggtttg ttcttcgtgc agcccgcgtt tttcggtggc
    14701 tgagttcagt gcgccgttcg tcgccgtaat cgtttctccc aatcgcgtca cgagtgtgct
    14761 tcggaaatgc cggaaggttt cgtccaccgt ccggagcatg gcccaccgga ggttctcggc
    14821 gttcgaaatc acgagatctt caagctgatt gttgagacgt gtctggaggc gatgcctgcg
    14881 cacccgttca gggagaaacc tgtcccagaa cccctccggg atggggctca gcgacgtaga
    14941 ccaggtccgc gcgatccagt agggcttctg cacgatatcg aaggtgatca ctttccctgc
    15001 cggttggtac ggcacctcga aaatgctggc cgctgctttc ctcacggatt cgaccagctc
    15061 ttccgccctg ctctggtgcg gcgtcagcac ggcgtcgatc tcttcaccta ggaaggcact
    15121 ggtctggccc accttctgtt cgaaaaagac gggaatcgct tctgcgaggg catccctcgc
    15181 atcgctttcg gcctgttgcg tatccgcgtt gcgagccaga gcgcctgcaa taatcccccg
    15241 gaagcctgtt cgcgcctgcg cccgcaccgc gtcaatgtac tcctccagcc ttgccctggt
    15301 gcgtctgtga tcgcctttga gcaggtcgtc cgcgacaagg cgatttcgtt cgatttcgtc
    15361 gagcttctgg cggaagaatg cgcgcttttt gtcgagttct tccgcgggga gctgcagact
    15421 tcgcagtata agtcgaaact gggtaagtgc ttcctcgaca gtgtcggccg ctttgtgccg
    15481 gaccgcctct gaaagagctg ctttcttttc cgaggcgagg aaccgggaaa gatgccgctc
    15541 gacccggtgc atgccgcttc tctcccagag gtcggcgtcg gaattctggc gggcattcaa
    15601 tcccaggtgc gcagaaaccg gaaaaatcgg gtggtcatca gaaaacccga gtttgtctgc
    15661 aagcaccgat ttcagaaagc ccgtggcttc gcgcacatcg gcttccgtat ggtagtcgat
    15721 cttgttcaac actacgaaaa gcctgttgat gcggcgcttc acctgccgca caaactcaac
    15781 ctccacttcg gtgatgggcg ggtctgccga cacaacgatc attgcggcat cgcactgcgg
    15841 gaggaagttg agcgttgctt cggtgttgtg ccggtgcgtg gatccgatgc cgggagtatc
    15901 tatcagaacg acgccctgtc tgaggatcgg cgcgctgctg aagacgtcca cctgagttac
    15961 tcctcgctca tttttcggat tgccgctttc cgtgacaaac tgggcgatga attcccgaat
    16021 tgcgcccgca tcatcagctt cgaacgcgcg gggcggcacg ccgttgtcga aatacacgag
    16081 cgcacgtctt gtttctcctg cgcgaacgaa ggtaggaatt gccgtgagcg gaaccactga
    16141 ggaggggaga atcgcttcgc cgagcagggc gttcaggagt gtgctcttgc cacgtttgaa
    16201 ctggccgagt actgcgaggt gaaatcgctc ctcggcaagg cgggttttca gttccgcaat
    16261 tgcgtcggaa aaggtcttga attctggtcc cgcagtgcca aggatcgaca gtgcctcatc
    16321 aaagagctcg ccgaggccat ttgcgttcgt gatatcgccg ttctccccgc cggcggtctg
    16381 cggggcccgg acatccgttt cagggataca cacagttgct cctcccgtcg ttgtggtgtc
    16441 ggcaggagtc atcagcccgg caagcccggg cggcgcgggg actccatccc ctcatgtggc
    16501 cggagagcct gcgctcgttt tcggagcgca acgctcgatt tcttccgtca gggacgtgac
    16561 ggaatccgtc aggcagttgc cgtggaaaga accggcatgt cgcgtccacg ccagagaaac
    16621 cagagcgttc ccgccagacc gaacgcggag gcgctgatga ggttcaccgc aggtgatatc
    16681 atccagagga acgccccgcc gaaagccgcg agcgacacga cggaatctct cagcaggtaa
    16741 tacaggccga acatccctgc cttggcacct tccggcgcga gatccatgat cagcgctttt
    16801 ctggtcggct cgccgaactc cttcaggccg cgcaggatga atgcaccgac gagcgggagc
    16861 agcgaatggc tgaacagaag cgccacagga aaaagggtga agttcacgaa ggtgattacg
    16921 acgaagggct ttttcccgct cttgtcagcg aagtatgcga cgggaatgta acacagcact
    16981 gcagtcgcca tttcgatcgt ggtgagaacg ccgaattcga tcgcgctgac gggattggcg
    17041 atgaccttca tgcaccagat aaccacgaat gcgtaaggaa tctgctcgca gaaccgcacc
    17101 aggatatcag atacgaggag gttcctgagg tccgcgttca tcagaccgat gaggcggaac
    17161 gggttgccgg aaaggggagc tgaagcctct tctcttttgt cctcaatcag caattgctgc
    17221 ataaccatcg ccactgcggc aaaaatgaac gcggcgatga atgctgctct cacaccctcg
    17281 cgttcgcccc agatcgagat gaatgcgccg cctaccagcg ggcccagcgc catgggaatc
    17341 cggcgcacga gggagtgcat cgaaacaccc atcgtccttt tgtttttcgg caggacaacc
    17401 gcgacgaggc ccatcgtggc cgggagggag atcgcggtcc atgagaggaa gaagaatgcg
    17461 ccgatgatca ctgcttgcca gaccgggatg aagatcacca cgcagtatcc ggtcatcgcc
    17521 atcaggttga acacgatcag ggcgcgcttc gtgccgatcc gatcggcgag gtatccgccc
    17581 gggaatgagt aaagggccga aaggaggtta tccagcccgt tcaggaggcc gacggaaaga
    17641 actcctccgc caagagcgaa cagatagatc ggcaggaatc gttcggccat gcgctcgccc
    17701 atgccgacca gcaccaccat gctcagcacg ccgatgatac ttcgcttcag gccaagaaaa
    17761 tcgattgcgt tcctgatcgc cgccatttca ggcgcctttc aaattgagaa gcgagcggtc
    17821 cctccgcagg gcgatgtgcc cgattgaggc gggagagccc tttcacttcg gaatatgcct
    17881 cccgcgcccg tgcgccccca acacctgttc tccctcggtg cggtgaaccg gccgcgtgat
    17941 ggttactctt ctccaacgtc ctcttcttct ttcgggggaa gcgccctgcg gccggcaggt
    18001 tccacggaaa ccggtgccgt ctccggcagg agatgccgcg gcatgaaggc cgcattcgca
    18061 ataagcgtcg gaatgacagc gctggcgatg actgtcgcca caaggaacga gtactgcgat
    18121 tgcgtcacga tgccgtgact gaatccgtag agcgcggaaa ttgtgccgaa cgtcaggccc
    18181 gtggacatca tgagtgtgta gtaccagcgt tcgtttttct ccgttctgaa tgccgagaca
    18241 accggcagaa gcccgaaaat cttcgagacg gttttcgccg ccaggaacac gatgaacact
    18301 gccggcgcgg cgatgagcgc gggaagcgcg accagagaac cggcgcgcag gaagtaaaac
    18361 ggtgtcagaa acccgaccgt gagggtacgg ag
//
LOCUS       DAOTNG010000015        18131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3112_length_18131_cov_4.825072, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000015 DAOTNG010000000
VERSION     DAOTNG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18131
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3112_length_18131_cov_4.825072"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="pgk"
                     /locus_tag="P5179_01530"
     CDS             <1..600
                     /gene="pgk"
                     /locus_tag="P5179_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRS93935.1"
                     /translation="GSKISGKIDVINNLLPKVDALLIGGGMAYTFYKAMGLEIGKSLL
                     EADKIDLARELLSKAGKKIVLPVDTVIADAFDNNATTKDAKRDEIPAGWEGVDIGPAT
                     VKEFGDIIKSAKTIVWNGPLGVFEMPNFAKGTAAIAHAVAEATDKGSISVIGGGDSAA
                     AIAQAGLAERMSHISTGGGASLEFLEGKVLPGVAALTQK"
     gene            837..1592
                     /gene="tpiA"
                     /locus_tag="P5179_01535"
     CDS             837..1592
                     /gene="tpiA"
                     /locus_tag="P5179_01535"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRS93936.1"
                     /translation="MRRRIVAGNWKMNKTAAEAAALVGELRPLVSDVSNVDVVVCPPF
                     TALAAVAPALVGTNIELGAQNMHWEKNGAYTGEVSAEMLLTLGCRYVILGHSERRQYF
                     HETNSEINKKVKAALAAGLTPILCVGETLEEREDGQTETIVKEHVTGGLLGLSPDQVG
                     KVVIAYEPVWAIGTGKTATPDQAQAVHAFIRNVLKQISNSAIADTVRIQYGGSMKPEN
                     AKELMGKPDIDGGLIGGAALKADSFAAIVKAAV"
     gene            2021..2108
                     /locus_tag="P5179_01540"
     tRNA            2021..2108
                     /locus_tag="P5179_01540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2056..2058,aa:Leu,seq:gag)
     gene            2264..3262
                     /locus_tag="P5179_01545"
     CDS             2264..3262
                     /locus_tag="P5179_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS93937.1"
                     /translation="MSVLRRRKWVAKSLLSSAAAIVLVMGCGHAPEVRVAPAPEGESG
                     ELASDVDFAQAYLDLGTRYYEAQRFADAIEQFRFALELNPYSDEAHALIGMSNYRLGK
                     RELAERSFRRALQLNSRNLLAQNGLALVSNDERERAAALESAITYNPDVPELRNNLCF
                     MYVQSGDVERAIQECEASVRLDSANAYSRYNLGYAYQRQGRFEEALEQYRRALEIRAE
                     WARVLNNIGLVHYYRSELSNAVEYYRRAIAAEGSEAIYHYNLALAYEAIGVRLASAEL
                     RGEPARNVYGVAADTDWREMLREAASEYSTYLELAPGVSDAQRIRAKVAELRARAG"
     gene            3273..5120
                     /locus_tag="P5179_01550"
     CDS             3273..5120
                     /locus_tag="P5179_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93938.1"
                     /translation="MRNRILIAPLTGTSGIPSISFGCPHFCVAAIVAVLISCFLANPA
                     SAQGSSSGFNPDAELARLRSTRANLERVQRMFYLGELTANSGREDVAQNAYGDVLSTF
                     ERLSGNARSLALPYAAQSALSLAAMTHVAYRDSPLRLERYAADSAQRAALLVRTREAY
                     NRALEFRYARATFEALFFRAYALQEWNTGGLDYAAERQPGTELLEQTIRDLNLSRQLL
                     DQAIREHQQILHLADSLGFSGGTGDRDVSEWVSLAREQLAAIPQQRDSLVAREEALQT
                     IYIERRASRWLARAEPLLWEKVTDLARRDARIADPFFDFVLQSRMVNDVFRPYIFGPS
                     GFLQAHDQARKGAQTVRSEEWMAERFAWRRRQEWRDGEIAMRLSREGLRHLLRSPEMV
                     DSVVVMLRTAADSLPGEARIALSRAAPQPPQIRMPDLPDLGGVDPRLLSGEEHAAKLD
                     EYQRYVTRMDSVSRQIEAYRAAVRSFSETVAAYVGQPLPSAADRYLRVRRALRATETL
                     LLDTLSALVLEQTRSALERSRLAAGWAPPGEVSASMQQTIADHAQTTSQNLRLAAAIS
                     REQAARYRVEVSRLRQTPLRDDLAETAATLEHFAGTLEQQAALFAMGTG"
     gene            complement(5182..6300)
                     /locus_tag="P5179_01555"
     CDS             complement(5182..6300)
                     /locus_tag="P5179_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93939.1"
                     /translation="MTLRERAMTVLRGGIPDAVPWFTDLTYYQHGTTQAGTLEAKYRG
                     SEGLLQLHRDLRVGVYLFTPPLVTIERDPTLFSIEEKDIGDGKTLRVFRSPEGSLTSV
                     TQSCADSFSSAILEYPVKTADDLRVVVAWYQGACYSPNYDAVLACDAHWGDDGLGVPL
                     TPRTPLAALVAEWCGVNNLAYLVADAPAEVEATLDAMRRAEDELYRLTCAAPYPLVEI
                     PDNLSAEAVGGLWRCFSRDYYRERVAALHASGKVVGCHIDGTLGHLFADLVEAGIDFP
                     ESVVPAPVGDLTLEQIRDASSPNTIIWGGIPSAMFAPPFERDWVLSFAKRVVAVLGEE
                     RGLVLAGADQVPPNGDIDLVRRIGDLLEELGPPTQIGS"
     gene            complement(6490..7925)
                     /gene="carB"
                     /locus_tag="P5179_01560"
                     /pseudo
     CDS             complement(6490..7925)
                     /gene="carB"
                     /locus_tag="P5179_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878769.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(7939..8601)
                     /locus_tag="P5179_01565"
     CDS             complement(7939..8601)
                     /locus_tag="P5179_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93940.1"
                     /translation="MPRFQVLRAIAGVLIGIAGLVSTPEAAVSSYYVCEQAAYLVNSV
                     GKAYGVSPALEAGLRFGSSSVGYRVSAAYTMLVSASDTLPAVVHHGSVAGDVIFPRAF
                     GAERLYGFAGIGFGTRRLPEGRIVCDERKVDAPWSPYGYYVVQDTLSGGFGARVFDLI
                     GRAGIGYVFHESPRWRGAVEVRYQAAFGVGNPPGITLDMRTDETIGAGVTIEWVRAYR
                     HR"
     gene            complement(8614..9147)
                     /locus_tag="P5179_01570"
     CDS             complement(8614..9147)
                     /locus_tag="P5179_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93941.1"
                     /translation="MTDTRTRVDELIEESYLISLPEDAIRKDVLQFLDSCERIAAQTR
                     DESKRQEMQRRLKMFRQLGLRLDSAAELSEEIDQWMAWSPHDMLAHRAARLKLLVLDA
                     LAAVDPNVPSRVGKERFPQSGPRGASDTGPVSRSNILPYPRRRKVARKLAVWALRVAY
                     GILLLSAIAVFTYRFLR"
     gene            9281..11257
                     /locus_tag="P5179_01575"
     CDS             9281..11257
                     /locus_tag="P5179_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93942.1"
                     /translation="MRPPWTSSTPFTYEIGYGQDYVGNEQFLSDVRRGRPTLLHCGHD
                     VPLNSATGNTIQFTPRDMKYLDPEGIPARMSELRQYVDSLHEAGVKWAVPYLITMLII
                     GDPEKRTGFWNLYDRWEEYSALGIGEKPGPPESWFSREPRPFPGHPGMLACETTISHP
                     EVRRFLHACTDLVARCGYDGVFLDVNTLIGRTPHDDRWFGEYLSTRYSESELKRRFGF
                     ASFGEVRLGEEGAGLLWVETQRFRAWAFGQLFAALRDAGREHVPGFFVLPNNSPMCTI
                     EGFFSRRGVGHGMRYIRNGCDLLMFEEMQQAGRFGADRIVDNILQYKMAAAHGMRGVV
                     LLYNATDRAAINLANAEAAAGGGGAFVQCGFAEADTMRFWREWFERHSSVLEGMDSLH
                     TVGVLHSLDEMYWDNRAHLEALYRLRQSLSDHHVLFDILLPEHLEQGKTSSFRILILP
                     EVRHLSNDQVAGMARFCRNGGKVILLGECGTFDETGARRDEKLCDVLDVSATSQVRSI
                     SDIVPTWGPELYELSEEDYNDFDLVARLPDRVASASEAEQVRRSALVPLLDTLLGEPC
                     TFLQDAPYSLRVSAFRSPDGRRTVVHLVNYDLPVLGKGMSGNPIPAGPVRIRYGGQRA
                     HWFTPEGLEGDAKRGGDGSFVIPAVETYCMVVIE"
     gene            11272..12438
                     /locus_tag="P5179_01580"
     CDS             11272..12438
                     /locus_tag="P5179_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93943.1"
                     /translation="MPVARQPGGQMTRNELIEALKRLYVDCLVDWDRNHADKLLTSED
                     SAELGWGEAYVLRAFVGAYRFTGDVYWLRRLCAHAGAVLANAKDTPDGKPFDPVYADG
                     FLGWGTYRYSGQYDEYMVHDGHICTPVAEFAAMVFADGRLYETFGEDATRFVRFIENH
                     IAAKWLNVWDRRRDGDEEFAWGETVRNWGGLDFIPHNQYAAFGALLVFLDDVVRDPRY
                     SSVMGAAAQTVYRQKAIEMGAYFKAHVESLPGTDAYAWKYHDTCGAEDTSHANLELEF
                     AWWLHERGLVFSDSDMDRFAGTFANVMWNGDAEDPRVNASTDGKGDWTGGKTTWGWAL
                     FSRRDPALWHVLALTFLSESEAPTGSGLVAMGRLLEACTEAGASLPTRGGMQRG"
     gene            12431..14878
                     /locus_tag="P5179_01585"
     CDS             12431..14878
                     /locus_tag="P5179_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93944.1"
                     /translation="MGKWRGHICAAIGVMWLSLAVPTSTASDVPGDFMARMIAWDGKY
                     ADSYHRTRDSGTLAWGESYVLRAFMSAYHVTKDPYWLRRVCTHADSLIAYAWDHPDTG
                     YYDPAYADGFLGWGTAHYSDQYDEYMVHEGHVMTPVAELVAEVFKDSTLWEEFGDRAQ
                     TYMAFLTDHIAAKWLRLWPGTPPASFKWGLTVREWYGLDYAPHNQYAVFGAMLLFMDD
                     VVRTARYAQLKPGDPPTIYRQRAVEMGEYFKRHLLYLPAYDAYSWKYHNNSGAEDASH
                     ANLELEFAWWLNERDLVFTDLDMQRFARTLTSIMWNKSLTAPAVNMSTAGTGDWSGTD
                     YLWGWSLFGKFDPLLWRILERYYAAKTGDTSPYGMATVARLQQFRDTFLNQGILVPER
                     LGVIDDGDRVIRPGETARIGVTVRNIGNARDSIIVRISGLDPLVIASGTDSVIAAVDS
                     GAAALVEFVVRAPVDVATKRVSIMARVGSLSVTGQVTVGSPSTVFVEGTPPADDGITH
                     NAFYAALTPPVHRWTWAEGDTFSTAALSRFEVVVWRAAWALPTPQQRDTLARFLDMGG
                     NLLITGGGVLPAWITRSPEDSLFFARYFKVTGAVDVHDSLAGLGVSVRPFDPMAFTAM
                     GTSRRYLRSSPVVHFDVLNSFGPPQKQIIFSATDAQFNPKVLPDSIGGVGIDSTYRAL
                     LFGWDLDDMSSNTSRTVVTLALTWLRSPTSVRAGEYPRPDAVREIELSVAPNPFNPHT
                     TITARGLKLGEPARISVVNVLGQVCWSTRNPRPGTEWRVMWDGRNVQGEFVGTGTYFV
                     AIEQGDARKATRIQLVR"
     gene            15028..17472
                     /locus_tag="P5179_01590"
     CDS             15028..17472
                     /locus_tag="P5179_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93945.1"
                     /translation="MRLSKRQGLVFVFATVVVGCLVRFAAAEDLPGVFTDRIYAWGAS
                     TKDSDAFLKTANSANLAWGESYVLRAFMSAYRLTGDAYWLRRVCTHADSLIAHARDFP
                     EGEYYDPVYADGYLGWGCKNYSDQYDEYMVHDGHVTTPVAAFVAEVYKDSTLWDEFGA
                     RAQTYFAFIADNIAAKWLAITPRTSSGTGETLRLWRGWSFLPHNQYAAFASLLLFLDD
                     AANTPRFAAVHPERVTTVYGQRAREMGTYFRSRLQLNPASNSYSWAYLGYGSGEDASH
                     ANLELELAWWLNERGVVFTDLDMERFARTLTTRMWNKSFNPPLVNKSTEGNDDWTGAE
                     GVWGWSLYSKFDPLLWRILERYYSALPTVRHHQVATLAHLQRFRDVFLNSGIILPEGV
                     IVIDDGDQVLRPGEKAKIGISVRNVGVQRDSAKVWLSQYAPLVIAPGGDTTSVVVEPG
                     ASARATFDVQVPGDVATRQITIVGYADFLPATGSVTTGYPDVAFVEGLPGADGLAHNQ
                     FYSSLTTVAHRWVHSETDTFTTVALRNFDAIVWRAAWAVPAPQQRDSLAKFLDMGGNL
                     LITGGGVLPAWIGRSTEDSLFFARYFKVTGAIDVHDSLQGLTPSVRAKNPAALSAIGG
                     SKRNFRTSPPIHFDVLHTFAPEDQTIIFAATDAQLVPKMLPDSVGGVGIDSTYNALLF
                     GWDFDDVSSVIATKSMITYLLAWFKSPTSVESGGTMRPTPTSALRIVASPNPFNPATT
                     ITARGLAQGIAARIAVTNVLGQECWSSTNARPGPEWSVVWNGRNQRGESMGTGTYFVV
                     IEQGDARKATRIQLVR"
     gene            complement(17845..>18131)
                     /locus_tag="P5179_01595"
     CDS             complement(17845..>18131)
                     /locus_tag="P5179_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93946.1"
                     /translation="LRVAVDFGHGAQSEKPEAPDFEGVSFVLVSAPLFDLFGRPFDAH
                     LPVAGSGIDLSALAGAPAEAVIAYDAEYSGWEDIYRDTKVIEEAAGRGGM"
BASE COUNT         3676 a         5219 c         5551 g         3685 t
ORIGIN      
        1 gggtccaaaa tcagcgggaa gattgacgtg atcaacaacc tcttgccaaa ggttgacgcg
       61 cttcttatcg gtggcggaat ggcgtacacg ttttacaagg ctatgggtct ggagatcggc
      121 aaatcgctcc tcgaagcgga caaaatcgac ctcgcgcggg aattgctttc caaagcgggc
      181 aagaaaatcg tcctgccggt ggacacagta attgccgacg cctttgacaa caacgccacc
      241 acaaaagacg caaaacgaga cgagattcct gccggctggg agggcgtgga catcggccct
      301 gcgacggtca aggagtttgg ggacataatc aaatcggcaa aaacgatcgt gtggaacggt
      361 ccactcggtg tgttcgaaat gcctaatttt gcgaaaggta cagccgccat cgcacatgcg
      421 gtggcagagg caaccgacaa gggatctatc agcgtcatcg gcgggggtga ttccgcggca
      481 gccatcgccc aggccgggtt ggcggaacgg atgtcgcata tttccacggg aggaggcgct
      541 tcattggagt ttcttgaagg caaagtactt ccgggagtag cggctctcac tcagaaatag
      601 gtctcgtcag cgctgtttcg gagaagcgtc gcccagtggt acgggcgacg cttctctgtg
      661 ttttccggct tcacgcgctc cacagcccga tctggcgtgt gaaggaaatg gcgcgggtat
      721 tgcgcatccg cgacatcatc ccgtattatt aaaatcttcc aaaatcctgt tttgcggtca
      781 gggtaaccca atcccggtag atttcaaccc gggttttcac gtcgggaggt ttctgcatgc
      841 gacgccgaat cgtggcgggc aactggaaaa tgaacaaaac ggctgcagaa gcggctgctc
      901 tcgtgggcga actgcggcct ctcgtgtcgg acgtcagcaa tgttgacgtc gtcgtgtgtc
      961 cgccgttcac cgcgctggct gctgttgcac cggcgctcgt gggaacgaat atcgaactcg
     1021 gcgcacagaa catgcactgg gaaaagaatg gggcctacac cggcgaggtg tccgcggaaa
     1081 tgctgctcac tctcgggtgt cggtacgtga tccttggcca ttctgaacgc cgccagtact
     1141 tccacgagac aaactccgaa atcaacaaaa aggtgaaagc ggcgttggct gcgggactta
     1201 cgcctatcct ctgtgtgggt gaaacgctcg aggaacggga agacggccag accgaaacga
     1261 tcgtcaagga gcatgttacg ggaggccttc tggggctttc gccggaccag gtgggcaaag
     1321 tcgtgatcgc gtacgaacca gtatgggcaa tcggcacggg aaaaacggcc actccggatc
     1381 aggcacaggc agtacacgcg ttcattcgca acgttctgaa acaaatctcg aactcggcaa
     1441 ttgcagacac cgtccggatt cagtacggcg gcagcatgaa accggaaaac gcgaaagagc
     1501 tcatgggaaa gccggacata gacggcgggc tcatcggtgg agctgcgctc aaggcggact
     1561 cctttgcggc catagtgaaa gcggctgtct gaccaccgag agacgccgga cttggaaaag
     1621 aggcgtgcca acatgatatt tttgaaaatc ctttttgtgg cggtctgtct gttcctgatg
     1681 ttgtccatct tgctccaatc gggaaaaggc gaaggattgt ccggaacgtt cggaggatcc
     1741 tcacagttca tgggtggacg cgcagctgcc acgtttctga ccaagacgac gaccgctctc
     1801 gcagcgacgt tcatgttgct gtgtgtcgtg attagcatat tgggcaaaag cgcggtcaca
     1861 gtgccggaag ggcaatcgga ggcccagcgc cgcctgtcgg agacggcgcc cgcccaggtt
     1921 gcgccgagtc cacaggctcc gtcgcaggca ccccagggaa aacccgcgcc ggtcccgacg
     1981 acaacccccg gagggcctca gtaacaaaga cacaccgcgt gcggaagtgg tggaatttgg
     2041 tagacacgta gcgttgaggg cgctatgctc gttagggcgt gccggttcaa gtccggcctt
     2101 ccgcaccata tcttctggac ccccgttgcc ggctgggtaa cgggggtttc attttcggac
     2161 caggtgcatg caatgtgaac cgggatagat attcgacgtt tcggcgtatc tataaggagc
     2221 ggaaactgcc gctggtaaag cacccacgga ggaatgtctg caggtgagcg ttctgagacg
     2281 aagaaaatgg gtggcaaaga gcctgttgag tagtgcggct gcaatcgtgc tcgtgatggg
     2341 ctgcggccat gcacctgagg tccgtgttgc gcctgcacct gagggggaga gtggtgaact
     2401 tgcctctgac gtcgattttg cgcaggcgta tctggatctt ggaacccggt actatgaagc
     2461 gcagcgattt gcagacgcca tcgagcagtt tcggtttgcg cttgaattga atccctactc
     2521 cgatgaggcg cacgccctca taggcatgtc gaattaccgt ctcggcaaac gagaactggc
     2581 cgaacggagt ttccgtcgcg cactgcaatt gaattcccga aatctcctcg ctcagaacgg
     2641 cctcgccctg gtctcgaatg atgagcgaga gcgtgccgcg gcactggaat cagccattac
     2701 gtacaatcct gacgtgcctg aactccggaa caatctctgc ttcatgtatg tgcagagcgg
     2761 cgatgtcgaa cgtgcgatcc aggaatgcga agcctctgtc cgcctcgata gcgccaacgc
     2821 ctactcccga tacaatctcg gatacgccta ccaacgacag gggcggttcg aagaggctct
     2881 ggagcagtac cgtcgtgccc ttgagattcg cgcggagtgg gcacgagttc tgaacaacat
     2941 cggcctggtg cattactacc ggagcgagct ttcgaatgcc gtggagtatt atcgccgcgc
     3001 gatcgccgcg gaagggagcg aagcgatata ccattacaac ctcgcactcg cgtacgaagc
     3061 catcggtgtt cgccttgcct cggcggaact gcggggagaa ccagcacgca acgtgtacgg
     3121 agttgccgcg gatacggact ggagagagat gcttcgcgaa gcggcgagcg aatacagcac
     3181 ctacctcgag cttgcaccgg gggtatccga cgcacagcgg atccgcgcga aggtcgcgga
     3241 actgcgggcg cgagccggtt gagggcgcag gtatgcgaaa ccggatcctg attgccccat
     3301 tgacagggac ttcgggtata ccctccatct cctttggatg cccgcacttc tgcgtggcgg
     3361 cgattgtggc cgtcctgatc tcgtgcttcc tcgcgaatcc cgcatcggcg cagggctcca
     3421 gttccggctt caatcccgac gcggaactcg ccagactgcg gtccacacgt gcgaatttgg
     3481 agcgcgtaca gcgcatgttt taccttggcg agctcaccgc gaactcaggt cgggaagacg
     3541 ttgcacaaaa cgcgtacggg gatgtccttt caacgtttga aaggctctca gggaacgcac
     3601 gttctcttgc gcttccctat gcagctcaat cagcgctttc gcttgccgcg atgacgcacg
     3661 ttgcctaccg ggactcccct ctgcgcttgg agcggtacgc ggcagacagc gcacaacgcg
     3721 cggcgctcct cgttcgtacc agagaagcct acaacagagc gttggaattc cgctacgcgc
     3781 gggcaacgtt tgaggcgctc tttttccgcg cgtatgcgct gcaggaatgg aacaccggcg
     3841 gcctcgacta cgccgcggaa cgacaaccgg gaactgaact cctcgagcag accatacgtg
     3901 acctgaatct ctcccggcag ctactggatc aggcgatcag agaacaccag cagatactcc
     3961 atctcgcgga ttcccttgga ttttcgggtg gcaccgggga tcgggacgtg agcgagtggg
     4021 tttcccttgc acgggaacaa ctcgccgcca taccgcagca gcgggattcc ctcgtcgcgc
     4081 gcgaagaagc gctgcagaca atttacattg agcgccgggc cagccggtgg ttggctcgtg
     4141 cagaaccgct tctctgggag aaagtcacgg atctcgcacg ccgggatgca aggattgcgg
     4201 atcctttctt cgattttgtg ttacagagta gaatggtcaa cgatgtgttc cgcccctaca
     4261 tttttggccc ctcgggtttc cttcaggcgc atgaccaggc ccgcaaaggc gcgcagacgg
     4321 tgcgctccga ggagtggatg gcggagcggt tcgcctggcg gcgaaggcag gaatggcgag
     4381 acggcgagat cgcaatgagg ctgtcgaggg aagggttgcg ccacctgctc cggtcaccgg
     4441 aaatggtcga cagtgttgtt gtgatgcttc gaactgctgc ggatagcctc cccggagagg
     4501 caagaatagc tctttccagg gcggcgccac agcctccgca aatccggatg ccggaccttc
     4561 cagacctcgg aggggtggat ccacggcttc tttcaggtga agaacacgcg gcaaaactgg
     4621 atgaatacca gcgctacgtc acccggatgg attccgtaag ccgtcagatc gaggcctaca
     4681 gagctgccgt gcggagcttc tctgaaacag tcgcggcgta tgtcgggcag ccgcttcctt
     4741 ccgccgcaga tcgatacctg cgtgtcagaa gggcactccg ggcgacggaa acgttgttgc
     4801 tcgatacgtt gtcagcactg gtattggaac aaacgcgttc cgcactggag cggtctcgtc
     4861 tcgcggcagg ctgggcgcct ccgggggaag tatcggcttc gatgcaacag acgatagctg
     4921 atcacgctca gacgacgtcc cagaacttgc gccttgcggc ggccatatcg cgtgaacagg
     4981 ctgcccggta ccgcgtggag gtctcccggc tgcggcagac gccgctccgt gacgatctgg
     5041 cggagacggc ggcaaccctg gaacacttcg ccggaacgct ggaacagcag gcggccctgt
     5101 ttgcaatggg aaccggctga acggcgaccg gaacagagcg cctgctctta tcctgtctcg
     5161 tcagccaggt cgcctgccaa ctcaagaccc gatttgcgtc ggcggcccca gctcctccag
     5221 aaggtcccca atccttcgta ccaggtcgat gtcaccattc gggggaacct gatcggcgcc
     5281 cgccaacacc aatcccctct cctcgcccag caccgctacc actctcttgg caaacgacag
     5341 aacccagtcg cgctcgaaag gcggcgcaaa cattgccgac ggtatcccac cccagattat
     5401 cgtgttcgga ctcgatgcgt cgcggatctg ctcgagagtg aggtcaccta ctggcgccgg
     5461 aaccacggac tcaggaaagt cgataccggc ctcgacaagg tcggcgaaca gatggcccag
     5521 cgtaccgtca atgtggcatc cgactacttt cccggaggcg tgcagcgcgg ccacacgttc
     5581 cctgtagtaa tccctgctga agcatctcca gaggccgccc accgcctctg cagagaggtt
     5641 gtcggggatc tccaccagcg ggtacggcgc cgcgcatgtc aggcggtaca gctcgtcttc
     5701 cgccctgcgc atggcgtcga gggttgcctc cacctccgcc ggtgcgtcgg caacaagata
     5761 cgccagattg ttcaccccgc accattccgc caccagcgcg gccagcggcg tgcggggcgt
     5821 caggggtacc ccgagtccgt catctcccca gtgagcgtcg catgccagaa cggcatcgta
     5881 attcggcgag tagcaagcgc cctggtacca cgcaacaact acccgcagat cgtcggctgt
     5941 tttcaccgga tattcgagga tcgccgatga aaaagaatcc gcacaactct gtgttacgct
     6001 cgtaagcgat ccttccggag aacgaaaaac ccgaagggtt ttcccgtcgc cgatatcctt
     6061 ctcttcgatc gaaaaaagtg tcgggtctcg ctcgatggtg accaagggcg gtgtgaagag
     6121 gtagacacct actcggaggt cgcgatgaag ttgcagtaac ccttcactgc cccggtactt
     6181 cgcctccaat gttcccgctt gcgtggtccc gtgctggtag tacgtaagat cggtgaacca
     6241 cggcaccgca tccggaatac cgccgcgcaa cactgtcatt gctcgctcgc gaagcgtcat
     6301 cctcattccc tccaggcata cagattgagg cggtactgag cgcacgagat caaaaagggg
     6361 cgggaaacgc gcccttcgtt cattccgaaa gccgcatgcc ttcgcttcag gttgctaacc
     6421 gatgggaacc cggtcggtcc aggatgtggc tatggcgtcg cgcgacgcag gctgtcacgt
     6481 cgcgaaggtg cgcaactggg ccgagaagtc cacgatctcc ttcatattgc gcaggaacca
     6541 ggggtcgatc tttgtcagct ccgccacatc ttccactgtg aagccggctt cgaaggcagc
     6601 ctgaatctgg aacaaccttg ctggcgtggg gacgggaagg tttgctttca gaaacgccac
     6661 ggcatcgtcc gccgggaccc cggcgtcttt tccgtccgat cccagcccgt gtcggtcgat
     6721 ttcaagtgat ctcaggccct tctgcagggc ttccttgaat gttctgccga tggccattgt
     6781 ctcgccgacc gatttcatct ggattcccaa ccggggttcc gcaccgggga atttctcgaa
     6841 tgcccaccgg ggaatcttga cgacgcagta atcgatggtg ggttcgaacg aagcgggagt
     6901 aaccttggtg atgtcgttcg ggatttcgtc cagtgtatat cccactgcca gcatcgcggc
     6961 gattttggcg atggggaatc cggtggcctt cgacgcaaga gcagaagacc tgctgacgcg
     7021 aggattcatc tcgataatca ccatgcgtcc ggttgcgggg tcgacggcga actggacatt
     7081 cgacccgccg gtttcaacgc cgatttcgcg gatgacagcg attgcggcgt ccctcatgca
     7141 ctgatactct ttgtcggtca gtgtctgcgc gggtgccaca gtgatggaat cgccggtatg
     7201 aataccatcg ggtccaggtt ttctatcgag cagatgatga cgacattgtc cgcaaggtct
     7261 cgcatcacct ccagttcgta ctctttccag ccgatgatcg attcctcgat aaggacctcg
     7321 tgaatgggac tgagctcgag accgtttgaa acgacgtcgc cgaattcctc cgtgttgtac
     7381 gcgatccctc ccccagttcc tccgagtgta taggatggtc ggattattgc cgggaaaccc
     7441 gtagtttcga ctatcttccg ggcttctgcg agggagtacg cgtacccact ccgcggcagg
     7501 tcgacaccga ttctgcgcat cgcctcgcga aacagcttgc ggtcttccgc tttgtgaatg
     7561 gcttccagct tcgcacctat caactcgaca ccatgcttct ccaatacgcc gctttcggcc
     7621 agttccactg ccgtgttgag agccgtctgg ccgccgacgg tgggcagcaa tgcatccggt
     7681 ttctcgcgct cgatgatttt cgcgcacacg tcagcagtga taggctctat ataggtgcgg
     7741 tttgcgaaat ccgggtccgt catgatggtt gcagggttgc tgttgacgag gatcacctca
     7801 tacccctgag ttctcagtgc cctgcaggcc tgagtacccg agtagtcaaa ctcgcacgcc
     7861 tgaccgatca ctatcgggcc ggacccgata agcattattc ggtgaatgtc tgttcttcgc
     7921 ggcatgattc ctcgtttgtc atcggtgccg gtacgctctg acccattcga tcgtcactcc
     7981 cgcacctatc gtctcgtccg tgcgcatgtc gagggtgatt ccgggcggat tcccgacgcc
     8041 gaacgccgcc tggtaccgaa cctcgactgc cccccgccaa cgcggagatt cgtggaacac
     8101 ataccctatt ccggcacgcc cgataaggtc gaacacgcgt gcgccgaatc cacccgaaag
     8161 ggtatcctgc accacgtaat agccgtaagg cgaccaggga gcgtccacct tacgctcgtc
     8221 gcacacgatg cgcccttccg ggagacggcg ggtgccgaag ccgatcccgg cgaacccgta
     8281 caatcgctcg gcgccaaacg cgcggggaaa aatcacgtcg ccggcgacac tcccgtgatg
     8341 tacgacggcc ggcaacgtat cgctggcaga taccagcatc gtgtaagccg ctgacacgcg
     8401 gtagcccact gacgaggagc cgaaacgtaa acccgcctcc aatgcggggg acacgccata
     8461 cgccttaccc acggagttca ccagatacgc agcctgctca cacacgtagt atgaagacac
     8521 tgcggcttca ggagtgctca cgagacctgc aatgccaatc agcactccgg cgatcgcccg
     8581 gagtacctgg aatcgcggca tcgcacccac tccttaacga aggaatctgt acgtgaagac
     8641 ggctatcgcc gagaggagaa ggatcccgta ggccacccgc aatgcccaca ccgccagttt
     8701 gcgcgcgacc ttgcgtcgcc ttggatacgg gagaatgttc gaacgggaaa cgggaccggt
     8761 gtcgcttgcg ccacgtgggc cactctgagg gaatctttcc ttcccgacgc gcgatggaac
     8821 attcgggtcg accgctgcaa gtgcatcgag cacgagcagt ttcaaccttg cggcccggtg
     8881 cgcaagcatg tcgtgaggcg accaggccat ccactggtct atctcctcgc tcagttccgc
     8941 ggcagaatcg agacgaaggc ccagttgtct gaacatcttc agtcgccgct gcatctcctg
     9001 ccgcttcgat tcgtcacgcg tttgtgccgc tattctttcg cacgagtcaa gaaactgaag
     9061 gacatccttc cgaatggcat cctcgggcag agaaatcagg tacgattcct caatcaactc
     9121 atcgactctg gtgcgcgtat ccgtcattgg tgctgcctct tcaatccggt acaccgggca
     9181 caaaagaatg tacgccaccg gcggcaaaac ggtcaagctg acgccctttg tatgagcgtt
     9241 gtcgccgcgc taccttagtc gaaggggaaa ggagacgccg atgagaccac catggacaag
     9301 cagtaccccg ttcacgtacg agatcggtta cgggcaggac tacgtcggca acgagcagtt
     9361 tctgtcagat gtgaggcgcg ggcgcccgac tttgctgcac tgcgggcatg acgttccgct
     9421 taacagcgct acgggcaaca caattcagtt cactccccgg gacatgaagt acctggaccc
     9481 tgagggtatt cctgcccgca tgtccgagtt gcgtcagtac gtggattccc tgcacgaggc
     9541 gggcgtgaag tgggcggttc cgtacctcat aacgatgctc atcatcggtg acccggagaa
     9601 aagaaccgga ttctggaacc tttatgaccg gtgggaggaa tactcggctc tcggcatcgg
     9661 ggaaaaaccc ggaccaccag agagttggtt ctcgcgtgag ccgcgtcctt tcccgggcca
     9721 cccgggcatg ttggcgtgtg aaacaactat ctcgcacccg gaggtgcggc gtttcctgca
     9781 cgcgtgcacc gaccttgtgg ctcgatgcgg gtacgacggt gtttttctcg acgtcaatac
     9841 ccttataggg agaacccctc acgacgatcg ctggtttggt gaatacctgt cgacccggta
     9901 ctccgaaagc gagttgaaac gaagattcgg atttgcatca tttggcgagg tgagactggg
     9961 agaagagggt gccggcctgt tgtgggtgga aacacagcgg ttccgagcgt gggcgttcgg
    10021 ccagcttttc gcggcgctcc gagatgcggg acgcgagcac gtgcctggtt tcttcgtgct
    10081 tcccaacaat tcgccgatgt gcaccatcga gggatttttc tcgcgcaggg gcgttggcca
    10141 cggtatgcgg tacatccgga acggatgcga tctgctgatg ttcgaggaga tgcagcaggc
    10201 gggcagattc ggcgccgacc ggattgttga caatatcctg caatacaaaa tggcagccgc
    10261 gcacgggatg agaggtgttg tactcctgta caatgcgacg gacagggcgg caatcaacct
    10321 cgccaatgcc gaagcggcgg ccggtggagg tggcgcattt gttcagtgcg ggtttgccga
    10381 agccgatacc atgaggttct ggcgcgagtg gttcgagcgg cattccagtg tgttggaagg
    10441 aatggattcg cttcacacgg tcggcgtact ccactcgtta gacgagatgt actgggataa
    10501 ccgcgcccac ctcgaggcgc tgtaccgtct tcggcaatct ctgtcggatc accacgtgct
    10561 gttcgacatc ctgctgccgg agcatctgga gcaggggaag acttcgtcgt tccggattct
    10621 cattcttccc gaggtgcggc atctgagcaa tgaccaggtg gccgggatgg cgaggttttg
    10681 ccggaatggc gggaaggtaa tcctgctcgg tgaatgcgga acgttcgatg aaacgggagc
    10741 acgacgagac gaaaagctgt gtgatgtgct cgatgtgtcc gcaacctcgc aggtgcgttc
    10801 gatatcagat atcgttccga cgtggggacc ggagctgtac gaactcagcg aggaggacta
    10861 caatgacttc gacctcgtcg cccgcctgcc cgaccgcgtt gcttccgctt cggaggccga
    10921 acaggtgcgg cgttcagcgc tggtaccact tctcgacaca cttctgggtg aaccctgcac
    10981 gtttctgcag gacgcgcctt acagtctccg cgtttcggca ttccgttcgc cagacgggag
    11041 gcgaaccgta gttcatctgg tgaattacga ccttcccgtg ctcgggaaag ggatgtccgg
    11101 taacccgatt cctgccggtc ccgtgcgaat acgttacgga ggtcaacgcg cacactggtt
    11161 cactcccgag ggtctggaag gcgatgcgaa acgcggcgga gacgggagtt ttgtcatacc
    11221 cgctgttgag acctactgca tggtggtgat tgagtgagtg gacggccgaa catgcccgta
    11281 gcgagacaac ccgggggaca aatgaccagg aatgagctta tcgaagctct caagagactc
    11341 tacgtggatt gcctggttga ttgggacagg aaccacgccg acaaactgct taccagtgag
    11401 gattctgcgg aactgggatg gggcgaagcg tacgtcctgc gggctttcgt tggagcatat
    11461 cgctttaccg gcgacgtcta ctggcttcga aggctgtgtg cgcacgcagg agccgtactg
    11521 gcaaacgcga aggacacgcc ggacggcaaa ccgttcgacc cggtgtatgc tgacgggttc
    11581 cttgggtggg gaacgtacag gtactctggc cagtacgacg aatacatggt gcacgacggg
    11641 catatctgta ctccagttgc ggaatttgcc gcgatggtat tcgctgatgg gcgtctgtac
    11701 gagacgttcg gggaagatgc gacgcggttt gtcaggttca ttgagaacca tatcgctgca
    11761 aaatggctca acgtgtggga ccgtcgaagg gacggtgacg aggagttcgc atggggagag
    11821 acagtgcgga actggggcgg actggatttc attccccaca accagtacgc agcctttggc
    11881 gctctactcg tgtttctgga tgacgtggtc agggatcccc gttactcttc ggtcatgggt
    11941 gccgccgctc agaccgtata tcgccagaag gccattgaga tgggcgcgta cttcaaagcc
    12001 catgtcgaat cgcttccggg gaccgatgcg tacgcgtgga aataccacga cacctgtggc
    12061 gccgaagaca cttcccacgc caacctcgag ctggagtttg cctggtggtt gcatgagcgc
    12121 ggacttgtgt tctccgacag cgacatggac cggttcgccg ggacgtttgc caacgtgatg
    12181 tggaacggcg atgcggaaga tccccgcgtg aatgcgtcga cggatggaaa aggtgattgg
    12241 acgggaggca aaacgacatg gggatgggca ctcttttccc ggcgcgaccc cgctctgtgg
    12301 cacgttctcg ccctaacgtt tctttcggag tctgaagcgc cgaccggtag cgggcttgtg
    12361 gcaatgggcc gtttgttgga agcctgtacc gaggcaggag ccagcctgcc aaccagagga
    12421 ggaatgcagc gtgggtaaat ggcgcggaca tatctgtgca gcgatcggtg tgatgtggtt
    12481 gagtctggct gtgccgacct cgacagcatc ggacgtgccc ggcgatttca tggcccgcat
    12541 gatcgcgtgg gacgggaaat acgccgattc ataccacaga actcgggatt ccgggactct
    12601 ggcgtggggg gaatcgtacg ttcttcgcgc attcatgagt gcgtatcacg tgacgaaaga
    12661 cccgtactgg ttgaggcgag tgtgcaccca cgcggattcg ttgatagcct acgcgtggga
    12721 tcatccggac acgggatact acgacccggc ctacgccgat ggatttcttg gctggggaac
    12781 ggctcattat tctgaccagt acgacgaata catggtgcac gaagggcacg tgatgacacc
    12841 cgtcgctgag ctggtcgcgg aggtattcaa ggactcgacg ctgtgggagg aattcggaga
    12901 tcgggcgcag acgtatatgg cctttctcac cgaccacatt gcagcgaagt ggctgcgcct
    12961 gtggccaggc acgccgccag cgagctttaa atggggtctc acagtgcgcg agtggtacgg
    13021 gctcgattac gcaccccaca atcaatacgc ggtgttcggc gcgatgctgc tgttcatgga
    13081 cgacgttgtt cgaaccgcac ggtatgcgca gttgaaaccg ggtgacccgc ccacgatcta
    13141 ccgccaacgc gcagtggaga tgggcgaata cttcaaacgg catcttctct acctgcctgc
    13201 gtacgacgcc tactcgtgga aataccacaa caacagcgga gccgaagacg cgtcgcacgc
    13261 gaacctcgaa ctggaattcg cgtggtggct gaacgagcgt gacctggtct tcaccgacct
    13321 tgacatgcag cgcttcgcgc gcaccttgac gtccatcatg tggaacaaaa gcctcactgc
    13381 acccgccgtg aacatgagca ccgcgggaac aggtgactgg tcggggacgg attatctctg
    13441 gggttggtcc ctcttcggca agttcgatcc tctcctgtgg aggattctcg aacggtacta
    13501 cgccgcaaaa acaggagata cctcgcccta cggtatggcg accgttgcaa ggcttcagca
    13561 gtttcgtgac acatttctga accaaggtat tcttgttccc gaacgactgg gcgtcattga
    13621 cgatggcgat cgtgtgattc gtccgggaga gacggccaga atcggtgtca ccgttcgcaa
    13681 tatcgggaac gcgcgggact caatcattgt acggatttcg ggtcttgatc ctctggtcat
    13741 cgcatccggg accgattcgg tcatcgcagc ggtggattcg ggcgctgcag cactggtgga
    13801 gtttgtcgtt cgcgcacctg ttgacgttgc gacgaaacgg gtgtccatca tggcccgcgt
    13861 tggttccctg tcagtcaccg gtcaggtgac cgttggctca ccatctactg ttttcgtgga
    13921 agggacacca cccgcggacg acggaattac gcacaatgcg ttctatgccg cgctgacacc
    13981 cccggtgcac cgctggacat gggcggaagg ggacacgttc tcgacggcgg cgctttcgcg
    14041 ttttgaggtt gtggtctggc gcgctgcgtg ggcgctaccg accccacagc aacgtgacac
    14101 gctcgcacgg ttcctggata tggggggcaa tctcctgatc accggcggtg gggtcttgcc
    14161 cgcctggatt acgcgatcgc cggaagattc cctctttttc gcccgatatt tcaaggtaac
    14221 cggcgcagtc gatgtgcacg acagcctcgc cggactgggt gtttctgttc gcccctttga
    14281 tcccatggcc ttcactgcaa tgggcacgtc caggagatac ctccggagtt cccccgtcgt
    14341 tcatttcgat gttcttaact cctttggtcc gccgcagaag cagatcattt tctccgcgac
    14401 agacgcccag ttcaacccga aagtcctgcc cgattcgatt ggcggcgtag gtatagacag
    14461 tacgtaccgg gctctgttgt tcggttggga tctggatgat atgtcctcca acacgtccag
    14521 aaccgttgtg acgctcgccc ttacgtggct gagatcgcca acctcggtcc gggccggaga
    14581 atacccacga cccgacgcgg ttcgcgaaat tgaactcagc gtggcaccga atccgttcaa
    14641 cccgcatacg accataaccg ctcgcggcct caagctgggg gaacctgcac ggatcagtgt
    14701 ggtaaacgtt ctgggacagg tgtgttggag cacgcgaaat ccccggcccg gtacggaatg
    14761 gcgcgtcatg tgggatggcc ggaacgtgca aggggagttc gtgggcacgg gaacgtactt
    14821 cgttgccatc gaacagggtg atgcaagaaa ggcaacgcga atccaactgg ttcggtagtt
    14881 ggcagatttg gggaccagag gtgtgtggtg catcccttga attcccgcga acgcacgcgt
    14941 gatttccggg ggaagctttc aaacaagcag ttcgttgcgg ggaaccgttc cgcaagagag
    15001 ggccgatcaa aagcgagggg tgagggaatg aggttgtcaa aaaggcaggg gttggtgttc
    15061 gttttcgcga cggtcgttgt ggggtgtctc gttcggtttg cggcggcaga ggatcttcct
    15121 ggagtgttca cggaccggat ttatgcctgg ggcgcgagca cgaaggattc ggatgcgttc
    15181 ctgaaaacgg caaactccgc caatctcgcg tggggcgaat cgtacgtgct tcgtgcgttc
    15241 atgagcgcct accgtttgac gggagatgcc tactggcttc gccgcgtgtg cacacacgcg
    15301 gactcgctga ttgcccatgc gcgggatttt ccggaagggg agtactatga cccggtgtat
    15361 gcggacgggt atctcgggtg ggggtgcaaa aactattccg atcagtatga cgaatacatg
    15421 gtgcacgatg ggcacgtgac gactcccgtt gccgcgtttg tcgccgaagt atacaaggat
    15481 tccactctgt gggacgaatt cggggcacgt gcgcagacgt acttcgcgtt cattgcggac
    15541 aatattgccg ccaaatggct ggcgattacg ccgaggactt cctcgggaac cggggaaacg
    15601 ctgcggcttt ggcggggctg gtcgttcctg ccccacaacc agtatgcggc gtttgcttct
    15661 ttgcttctct tcctcgacga cgcggctaac acgccgcgat ttgccgcggt gcacccggaa
    15721 cgagttacga cggtgtacgg gcaacgggcg cgcgaaatgg ggacctattt ccggagtcgc
    15781 ctgcagctca acccggcatc gaactcgtat tcctgggcct atctcggata cggttcgggc
    15841 gaggacgcgt cccatgcgaa tctggagctg gaactggcat ggtggctgaa tgaacgcggc
    15901 gtcgtgttca ctgatctcga catggaacgc tttgcccgaa cgctgacgac gaggatgtgg
    15961 aacaaaagct tcaatcctcc gctggtgaac aagagcaccg aaggcaacga cgactggacc
    16021 ggggcggaag gcgtctgggg gtggtccctc tattcgaagt tcgatccgtt gctctggcga
    16081 atcctggaac ggtactacag cgcgctccct accgtgcgac accatcaggt ggcaacactg
    16141 gcccaccttc agcgtttccg cgacgtgttc ctcaacagtg ggatcatttt gccagaaggc
    16201 gttattgtga tcgacgatgg tgaccaggta ctccgaccgg gagaaaaggc gaagatcggt
    16261 atctcagtgc ggaatgtagg tgtgcagcgt gattcggcga aggtctggtt atcgcaatac
    16321 gctcccctcg tcattgctcc ggggggtgat accacttctg tcgtggtcga gccgggggcc
    16381 tctgcccgag ccacatttga tgttcaggtt cccggggacg ttgcgaccag acagataacc
    16441 atcgtgggtt acgcggattt cttacccgct acggggagcg tcaccaccgg gtacccggac
    16501 gtcgcgtttg tggaagggct ccctggcgcc gatggactcg cccacaacca gttctattcc
    16561 agcctcacta ctgtcgccca ccgctgggtc cattcggaaa ccgacacctt cacgacggtt
    16621 gccctgcgga acttcgacgc gatcgtatgg cgcgccgcgt gggcggtgcc tgctcctcag
    16681 cagcgggatt cgctggcaaa attcctcgac atgggtggca atctcctcat aacaggtggc
    16741 ggtgttctgc ccgcatggat tgggcggtca acggaggatt cgctgttctt cgcccggtat
    16801 ttcaaggtga ccggcgcgat tgatgtgcac gacagcctcc aggggcttac cccgagcgtt
    16861 cgtgcgaaaa acccggcggc gctcagcgca atcggagggt ccaaaagaaa tttccgtacc
    16921 tcgccaccca tccatttcga cgttctgcat acatttgcgc ccgaagacca gaccatcatc
    16981 ttcgcggcga cagacgccca gttggttccg aaaatgcttc ccgattccgt cggcggagtg
    17041 ggcattgatt ccacctacaa cgcgctgctg ttcgggtggg attttgacga tgtgtcctcg
    17101 gtgattgcca caaagtcgat gatcacttac ctcctcgcat ggttcaaatc gccgacgtcg
    17161 gtggagtcgg gtggaacgat gcgcccgacg ccgacaagtg cactcagaat cgttgcatcg
    17221 ccgaacccgt tcaatccggc taccacgata accgctcgcg gcctcgcgca ggggatcgct
    17281 gcgcgaatcg ccgtgaccaa cgtgctcggg caggagtgtt ggagttcgac gaatgcacga
    17341 cctggcccgg agtggagcgt cgtctggaat ggtcgaaacc agcgtgggga gtccatgggc
    17401 acgggaacgt acttcgttgt aatcgaacag ggtgatgcaa gaaaggcaac gcgaatccaa
    17461 ctggttcggt agccggtacg acgggaggca ggggggaacg cggtgcggtg ttcgaaagcg
    17521 gagcgcagag cgcgcgagcg gtcctcgaag agacatgaac gagacagcga acgcagtggg
    17581 gatcaggctc ggttgcaccg tggacaggca ggaatgcctt gctgccttgt caggcagcct
    17641 gcccacccgt ttgcgggtcg gtggggcagg cattcctgcc tgcccgtgaa tgacgatgcg
    17701 cccgggggcg atgcccgcgg gctgcgttat gtcggccctt caggccttgg cggttgtcac
    17761 aagactcagg cgagccccga cggggcgcag taccatagcc cggctcaacg ggccgggcga
    17821 aagagggcct atcggacgaa tccgtcacat cccgccccgc cccgccgcct cctcgatcac
    17881 cttcgtatcc cggtagatgt cttcccaccc agagtattcc gcatcgtagg cgatgacggc
    17941 ctccgcgggc gccccggcaa gcgcggagag atcgatgcca cttcccgcaa ccggcaggtg
    18001 ggcgtcaaac ggccgtccga acaggtcgaa caacggcgcg gagacaagga cgaacgacac
    18061 gccctcgaag tccggcgcct caggcttctc actttgcgcc ccatgcccga aatcaacggc
    18121 aacgcgcagg t
//
LOCUS       DAOTNG010000016        18116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3117_length_18116_cov_4.530812, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000016 DAOTNG010000000
VERSION     DAOTNG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_18116_cov_4.530812"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1378
                     /gene="hydG"
                     /locus_tag="P5179_01600"
     CDS             2..1378
                     /gene="hydG"
                     /locus_tag="P5179_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRS93947.1"
                     /translation="MDEAHIEGLLSSSRSADKSEIREILDRAKELHGLTHEEVARLLM
                     VEDPDLLAEMGHAARWVKEEIYGRRLVFFAPLYMSNECVNNCLYCAFRRDNKEVPRAT
                     LTMDQIREEVEACERIGQKRLLLLVGEFGPLSQIVDYLEQAIATVYATKKGPDEVRRV
                     NINVPALDVEHFKRLKAAGIGTYQLFQETYRRSTFERAHPGGPKSRYDYHLQAMDRAQ
                     LGGVDDVGIGALLGLDDYRFEVLAMVMHAEHLDRTFGTGPHTISVPRINPAENAPWAM
                     NPPHPLTDEEFKKVVTILRLAVPYTGLILSTRENPSLRREVFALGISQLSAGSRTQPG
                     GYKADDPEELAQFSLGDHRPLDEVLREVASLGYIPSFCTSCYRSGRTGMDFMDLAKPG
                     LIKQYCLPNALSTFAEYLEDYASPETKEIGIKAIEEHIKGIDSEALRRTSRALVDRVR
                     KGERDVMV"
     gene            1783..2340
                     /gene="nuoE"
                     /locus_tag="P5179_01605"
     CDS             1783..2340
                     /gene="nuoE"
                     /locus_tag="P5179_01605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRS93948.1"
                     /translation="MISTTEFASIARLDNGHKNREVDLAAVDEILDSYKPLAPHALIP
                     ILQDVQEHYGFLPEAALDRIGRALDISTTRIFGVATFYNQFRLHPLGKHVIRICRGTA
                     CHVKGSAALLDTLEMELNIKAGSTTKDGLFSIETVACIGACSIAPVAMVGDKFHGRLT
                     VKDIPRLVKSLKREDAKGAAEKAVA"
     gene            2560..4356
                     /gene="nuoF"
                     /locus_tag="P5179_01610"
     CDS             2560..4356
                     /gene="nuoF"
                     /locus_tag="P5179_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRS93949.1"
                     /translation="MAAGAQAVYDAFLAEIAARNLSVDVMPVGCYGYCEKEVLIDIQL
                     PGKPRVFYANVTPQLAKRLVEHVLIEGKCASDIALGWLGNDGVVMPEGSDLPRITDIP
                     FFKPQLKIVLENCGLVDPDSLDSYIATNGFSALTKVLKKMKPEEVIAEVQASGLRGRG
                     GAGFPTGKKWEFANKAKGDRKFIICNADEGDPGAFMDRSVLESDPYRVIEGLVIGAYA
                     IGAHEGYIYIRAEYPLAVARLKRCIADAETAGLLGDNILGTGFDFHLKLKMGAGAFVC
                     GEETALIASIEGSRGMPRPRPPFPANSGLWGCPTNINNVETLANITTIINRGGTWFAG
                     IGTPGSKGTKVFALTGRINNTGLVEVPMGLPLREVIYEIGGGIPGGKRFKAVQLGGPS
                     GGCVPESLLDTPVDYESIKGTGAIMGSGGMVVMDESTCMVDVARYFMSFVQGESCGKC
                     IPCREGTKRMLEILERVAMVKPERNTDDQLRRFDGAMLLESLAHIVADTSLCGLGQTA
                     PNPVLSTLKYFRDEYEAHLFDRKCPAGVCRGLLTYRIDTSKCTGCGLCAAKCPQGAIL
                     GEKRQAHFIVSERCISCDACRQVCNFGAVLAE"
     gene            4494..6218
                     /locus_tag="P5179_01615"
     CDS             4494..6218
                     /locus_tag="P5179_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRS93950.1"
                     /translation="MPTVDLTINGREITVEAGTTILQAARLAGFQIPTLCYLSGLSPT
                     GACRVCVVEVEGARTLVPSCAFPVADGMVVRTHTARVRAARTAIVQLLLANHPQECLT
                     CVRNGDCELQTLTRELNIREIPYQGERRLAPVDSSSPAIERDPEKCILCGRCVRVCQE
                     IQNVGAISFTKRGFQSEVAPAYDRQLDITSCVNCGQCIIQCPTGALREHSSVRKVWEW
                     LQDPNMHVVAQTAPAVRVGIGEMFGAAPGGIYTGQLVTALSNIGFDRVFDTNFAADLT
                     IVEEATELVRRVKNDGPLPMITSCSPGWIKYCEHYYPDLIPHLSTCRSPHEMLGALIK
                     SYYAEQYGIFPGNIAVVSIMPCTAKKFETERPELETNGFPSVDAVLTVREIARMIKTA
                     GIDFLNLPPRDFDNPLGTSTGAADIFASTGGVMEAALRTAAFYLTGKELANVDFRDVR
                     GLRGIKEATIKIGDMDVNVAVASGLANAKVLMDQIRKGESPYAFIEIMGCPGGCINGG
                     GMPQGSNVETVRARMRAIYTIDQTKVYRRSHQNPAIRKLYEEYLGEPGSERAHHLLHT
                     TYMARERS"
     gene            6238..6657
                     /locus_tag="P5179_01620"
     CDS             6238..6657
                     /locus_tag="P5179_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS93951.1"
                     /translation="MAETVLVIDDDQDFLEATETLLDAMGFNVVTAASSSAAMEAVEK
                     VTPDVILLDVMMERADSGFTLAQRFKRKPELAKTPIIMVTGVSEHLGHRYSLDTPQER
                     QWIRADLLLEKPLRPEDLLGHIRQVLEQAHENSAAHH"
     gene            complement(6777..7661)
                     /gene="dapA"
                     /locus_tag="P5179_01625"
     CDS             complement(6777..7661)
                     /gene="dapA"
                     /locus_tag="P5179_01625"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRS93952.1"
                     /translation="MLQLRGSFVALVTPFTSDDRVNHALLRELVEWHIEQGTDGLVPV
                     GTTGESPTLTYEEHEAVIETAVEAAAGRIPVVPGTGSNSTREAVHMTQHAKLVGADGA
                     LIVLPYYNRPSQAGLIAHIERLNEVGIPLVLYNIPSRTGINMEPETTEKLSYLDNVVG
                     IKEASGNINQMSEVVIRTRGRCAVMSGDDALTLPLLSVGGTGVISVVANLFPREMARL
                     VAAWEEGKREEALELHQRLFLLSKALLALETNPGPIKEAMSMVGYPVGHVRQPLAPLS
                     AANRERLRKLLVEAGLLS"
     gene            complement(7874..8047)
                     /locus_tag="P5179_01630"
     CDS             complement(7874..8047)
                     /locus_tag="P5179_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93953.1"
                     /translation="MSRGDLNIGRMEGKQILKPRVGKSSTSRRRIGRVTSKARRRCAK
                     AELLFESWWMREA"
     gene            complement(8245..10503)
                     /locus_tag="P5179_01635"
     CDS             complement(8245..10503)
                     /locus_tag="P5179_01635"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93954.1"
                     /translation="MEICPLFVENVNRWPLPSFPVTFGVPLAAGTLLPGAPLVLRDSS
                     GKLFPVQSKSLAIHPDGSVRRLLVTAEPPVPGQKREAFSLESGGPEASETVGWNETRS
                     EWASIVVERQGDTLVIERGGQGWRLSVDGALTVEGFNHSLVGSFRIDTLSATDSGPVR
                     LRLDVAGAIWNGTTRLLDVDLVIQIFSRTPVLALDVIATNRTNREPVAVDHLRAKLER
                     LGGEYDPSVKVEVRNGPAEREVELPCAVRTDKLATSVFHGNEEKMRRASRLIGYGETG
                     FSRVGQSGEIVLAIRNFWENYPAGVAFDSDAIEIELFPRWAPPMQLHLGVGKTHEILV
                     ALLNPEDRECFPARGLVYSFGKPPLVQVPFEVRARAGTLSEYLPYMPETYPRMETNLF
                     DLFQNRIRGFGKLNWGDDYSPLYTNQKRGGEDVVWNNLEGDHPYHAFCQFARTGQFAY
                     FKDFHDSILHWMDVDFAERHADPRHEGALIAHTRRHVINGGTTPSHNWAEGFKEWYFM
                     TGDPRVERVLNQMADWFLRNKDRELRLPPYVRTWGWGLIQLAAIWEVTNRDELLELMQ
                     WMVEQLHTYCAEHEGLTQGFGETPGASSVNAFHSATVAIGAYHVFKISRNEIAKNLCL
                     EVTRSISDRRATSPEGIPTYVNGPEQNFPMQQAATLTLGAIGCAWELTRDKDLVRRGM
                     RMLEYCLDRGLQVDHMRIPGQFVEFEDGICLMPELVWPNCQLLSYQLRGILLFMKAAH
                     ECGYLKEVDYRF"
     gene            complement(10528..11511)
                     /gene="trpS"
                     /locus_tag="P5179_01640"
     CDS             complement(10528..11511)
                     /gene="trpS"
                     /locus_tag="P5179_01640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRS93955.1"
                     /translation="MRHKRLFSGIQPTGELHLGNYFGAITNWVRLQDQWDAIYCVVDY
                     HAMTVEYDSDTMRRRVLEVSALLIACGLDPAKCSLFVQSDVPEHTELMWVLSTLAPMG
                     RLQNMTQFKEKSEKNADNINLGLFAYPVLQAADILIYKAEAVPVGEDQLQHLELSREL
                     ARKFNTRFGETFPEPKQLMTEAPRILGLDGKSKMSKSLNNYIGLTETPEQVWEKLRPA
                     YTDPARLRKSDPGNPDICNIFTLHKMVSPKELVLEIDRACRKAEIGCIECKKHLAQNL
                     NNVLDPIRSRYSELEARPGEVLDVLAAGKERCRRTARETMDEVREKTGLRP"
     gene            complement(11702..12391)
                     /locus_tag="P5179_01645"
     CDS             complement(11702..12391)
                     /locus_tag="P5179_01645"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS93956.1"
                     /translation="MQATKARGKLTRREIKQDKLLIWFSRVSMVVQDNMWYIAGVILG
                     VVLLGTGYYIYRSWDARQTERGMAQVSGLEMLVRQEQYDRAITQADQIIGSFVGLPDR
                     MARLYKADALRGKGDYAGARAIYEKSKWDDEITSFHATKGLADCLGAEKQYERAGKLL
                     IEWVDDNKGSGFAPMALLEASTNLELALKYSEAQAALQRILDDYNESQLVGRARQRIR
                     MLEGAVQVTGS"
     gene            complement(12404..13948)
                     /gene="xylB"
                     /locus_tag="P5179_01650"
     CDS             complement(12404..13948)
                     /gene="xylB"
                     /locus_tag="P5179_01650"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRS93957.1"
                     /translation="MGLLLGLDIGTTGVKALVIDDSGHTLGRHTIEYPLLIPKPGWAE
                     QDPATWWTATITAIGSVLAEAGVKGADIAGIGLSGQMHSSVFLDEHDEVIRPALLWCD
                     GRTTKQRYWITERVGGDAEMRRLVSNPPLEGFTLPKVIWLRDEEPANFRRLKTLLLPK
                     DYVRFRLTGEKLMEVADASGTAMFDVANRVWSEELMRKVDLPMDILVPVRESVAECGR
                     ISRDVAAMTGLREGVPVAGGGGDNACGAVGTGIVREGRVLASLGTSGVVFAHTDTVKV
                     DPDLRAHTNCHSVPNRWCLMGVMLSAGGSLRWLRDVVADSERASASQSGQDPYDVMAE
                     EAAKAPIGSEGLVFLPYLSGERTPHRDANARGAFVGLSLRHGREHLMRCVFEGITFGM
                     RDSLSIMRELGLHISEIRATGGGARSAFWRQMQADVYGVPVVTVNASEGPAFGAALMG
                     GVCSGMFSDLADASDALVKIVSVSEPIPSNVSRYEEWYERYRALYPALKQEFANVATM
                     VEKHSQ"
     gene            14312..15265
                     /locus_tag="P5179_01655"
     CDS             14312..15265
                     /locus_tag="P5179_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRS93958.1"
                     /translation="MRSPLSSALAFLGSILLVTGGTAESQEASFSGHRNAVRSVACSP
                     DGRMIASASLDKTVRVWQTDQSNAMRVLGEFPGSVLSVAVGTEGHLLVLGCADNTIRV
                     CTCTDGAVLRTIVTTSGPVYSVAISPDGHYVASSGNDGSVRIWQLEDGTLVRTFAGHA
                     APVWSVAFSPDGRYLLSASSDHKVGVWNVADGALIRTLEGHTAPVFDVTVTPDGRYVL
                     SGGVDRTIRVWNLSTGTLIRTMRGHTGTVYGVAVNPGGTCVASSSADGTVRVWDISSG
                     LVQRTFSNETAIRDITFTGDGCNLVGGCDDKTVKIWSVRPS"
     gene            15486..16346
                     /gene="ispH"
                     /locus_tag="P5179_01660"
     CDS             15486..16346
                     /gene="ispH"
                     /locus_tag="P5179_01660"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRS93959.1"
                     /translation="MLKITRAKSAGFCFGVRRAVHTALETCQNTHKPVHMLGDIVHNE
                     RVVAQLREAGIRVVKSLDEVDDGVLLIRAHGAEPATYEEAKTRGLQIVDATCPLVKEI
                     HERARELHAAGYPLLVIGDHGHDEVKGIAAQVPDAIVVSTPEEVRSLGRKFRRIGVVA
                     QSTQDLDNVRAIVSELIPICIEIRFVNTICYPTAKHQMEIRAMPLENDVMIVVGSFTS
                     ANTKRMTQISRGLNPRTYQVTGADDLCADWFSGVNTVGIHAGASTPDSVVEEVIARIR
                     QLAGTPVEIV"
     gene            16343..17053
                     /locus_tag="P5179_01665"
     CDS             16343..17053
                     /locus_tag="P5179_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRS93960.1"
                     /translation="MTLRLCFKHFRDVRAVLLALSLCSLLNGYPREAHAADSFAGAAI
                     AQLQYGGGGDWYANPSSLPNLLKFARKEAGISVSPTPGRVRITDPELFAYPLIYMTGH
                     GNVRFSDEEVVRLRRYLLSGGFLHADDNYGLDESFRREMRRVFPDNALVELPFDHPIY
                     RSWFVFPNGLPKIHEHDGKPAQGFGIFAGGRLVVFYSYESDLGDGWEDQHVHNDPEEK
                     RLEALRMGTNILVYALTH"
     gene            complement(17083..>18116)
                     /locus_tag="P5179_01670"
     CDS             complement(17083..>18116)
                     /locus_tag="P5179_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93961.1"
                     /translation="EQGCELSPVTQETGATAPSVTPDGTHVYYFVDETRLDGGKLTLK
                     RMNLDGSAREVLYVLQGTVPGTPFFPSQIYPLSTIRSDGKSLALSCFLADMGSGRRTW
                     GLMVFDLESPSVTLILEGPSWCNVHPQYSRSIHPGSQRDILVQENHGNTHTPEGEIID
                     LTGGNGADVHVIRDDGSSFRNMPWGRDGNEFCQGHQCWRGNTAWAITSTDTRHPDEQQ
                     LIESVCVPHTGHDGARSPGAIRNNLSREFPSPSFSHFATDASGSWFVTDTFACDNGGR
                     VFVARMGNAGTDPLSSWTCIAYPRCSWRKDAHIHPFLSPDGKTAFFNSDESGVLQAYM
                     VRGLESLGI"
BASE COUNT         3787 a         5353 c         5157 g         3819 t
ORIGIN      
        1 catcgacgaa gcccacatag agggattgtt gtcttcatcc aggtctgccg acaaatctga
       61 gatccgagag atactggatc gggcgaagga actgcacggg ctcacccatg aggaagtcgc
      121 gcgtctgttg atggtcgagg acccggatct cctcgcggag atggggcacg ccgcacgctg
      181 ggtgaaagag gagatctatg gccgaaggct tgtgttcttc gcgccactgt acatgtcgaa
      241 tgagtgcgtg aacaactgcc tttactgcgc attccggcga gacaacaagg aggtgccgcg
      301 agccactctc acgatggacc agatcaggga ggaggtggaa gcgtgcgaga ggattggcca
      361 gaagcgcctg ctcctgctcg taggggaatt cgggcctctc tcccagatcg tggactacct
      421 tgaacaggca atcgcgactg tgtacgcgac aaaaaagggg cccgacgagg tgcgccgggt
      481 caatatcaac gtgccggctc ttgatgtcga gcatttcaag cgactgaaag ccgcaggaat
      541 cggcacgtac cagttgttcc aggaaacgta ccgtcgctcg acgttcgagc gtgcgcatcc
      601 gggaggcccg aaatcgcggt acgactacca ccttcaggcg atggacaggg cgcaactcgg
      661 cggagttgac gacgtgggaa taggcgcttt actgggtctg gacgactacc gcttcgaggt
      721 tctggcgatg gtgatgcatg cggaacacct cgaccgcaca ttcggaaccg gacctcacac
      781 gatctcggta ccccgcatca acccggcaga aaacgccccg tgggccatga atccgcctca
      841 tccattgact gacgaagaat tcaagaaggt tgtcacgatc cttcggttgg ccgtgccgta
      901 tacggggctg attctctcca cgcgcgagaa cccaagcctg cggcgggaag tcttcgcact
      961 cggtatttct cagctttcag caggctcgag aacgcaaccc ggcggataca aagcggatga
     1021 ccctgaagaa ctggctcagt tctccctcgg ggatcaccgc ccgctggacg aggttctacg
     1081 ggaagtcgcg tcgctcgggt acatacccag tttctgcacg tcctgttacc gatccggtag
     1141 aacgggaatg gatttcatgg acctcgcgaa accgggcctc atcaagcagt attgcctccc
     1201 gaacgcgttg tccacgtttg cggaatacct cgaggactat gcctcccccg aaacaaagga
     1261 aatcggcatc aaggcgatcg aggaacacat caaagggatc gattcggagg cgctgcgccg
     1321 cacgtcgcgc gcccttgtcg acagggttcg aaaaggtgaa cgcgacgtga tggtgtaacg
     1381 atcgcccatc acattgtgct tcctgctcaa ttgtgagata cggtgctcat ctatgatgcg
     1441 caccgttttt cttgtctgcg ttttccgacg cgttctcccg gatttcttgc cgtgggagaa
     1501 ccttgatgtg cgtgcacgca caacgcacgt ggggacgaaa aggaagtctg ccgcagactc
     1561 cccacagtcc ttacggcaac agaaacggtt cggtggaccc atctgcggcc ccatgcggat
     1621 gagtgtgatt gtctctgaca aacgtccggg gagcgatgtt gcggtacgag gtttgcactt
     1681 gtttcgcact cggtgtgaat gtccgcaatc ggacgcaccc gggttcgagg cttattctta
     1741 gtaactgggc cttttggatc aatccacaca gatgggagac ccatgatttc cacgacggag
     1801 ttcgcgtcta tcgcgcgcct cgacaatgga cacaagaacc gcgaggttga tcttgctgcc
     1861 gttgacgaga tccttgactc ttacaaaccg ctggcccccc atgcgctgat tcccatactc
     1921 caggacgttc aggagcatta tgggttcttg cccgaagctg cgcttgatcg gataggccgt
     1981 gcactggaca tctcgacaac ccgcattttc ggcgtggcga cattttacaa ccagttccgg
     2041 ctgcaccctc ttggcaagca tgtcattcgt atctgccggg gtactgcctg ccacgtcaaa
     2101 ggctccgccg cgttgttgga cactctggaa atggaactca acatcaaggc cgggtccacc
     2161 acaaaagacg ggcttttcag cattgaaacg gtggcctgta tcggtgcgtg ttccatcgct
     2221 cctgttgcca tggttggcga caaattccac ggacgtttga cggtgaagga tattccccgt
     2281 ctggtcaaat cgctgaaacg tgaagatgcc aaaggcgcag ccgaaaaagc ggttgcttaa
     2341 gagatgaggc caatgatgtt caaattgcta gaaccgtgtt gctccaagtg ctggcactcc
     2401 gcggagaaac gctgccccga tgtggtctac tgcgctctcc acgggcccca gtgccacgag
     2461 gacgcagcat gcagcgcact gcgcaaggaa cgtaaagacc gtgcttcagg agatctgctt
     2521 gagtgtcccg tggttcacat cggaatgggc acctgcggca tggcagccgg cgcacaggca
     2581 gtgtatgacg cattccttgc agaaatcgcc gctcgcaatc tttccgtgga cgttatgccg
     2641 gtcgggtgtt acggctactg cgaaaaggaa gttctgatag acatccagtt gcctggaaaa
     2701 ccccgcgttt tttacgcgaa tgtcactccc caacttgcga aacgtctcgt ggagcatgtg
     2761 ctgatcgagg ggaaatgtgc ctcagatatc gctcttggct ggcttgggaa cgacggcgtt
     2821 gttatgcctg aaggctcaga tctgccgcgc atcacggaca ttccgttctt caaaccgcaa
     2881 ttgaaaatcg ttctggaaaa ctgcgggctg gttgatccgg acagccttga ctcctacatc
     2941 gccaccaacg gtttcagcgc acttaccaaa gtgctcaaaa agatgaagcc ggaagaggtg
     3001 atcgcggagg tccaagcctc cgggcttcgg gggcggggcg gcgcaggatt ccccaccgga
     3061 aaaaagtggg aattcgcaaa caaggcaaaa ggcgaccgca aattcatcat ctgcaacgcg
     3121 gacgaaggtg accccggggc gttcatggat cggagcgtac tggagagtga cccctatcgt
     3181 gtgatcgaag gactcgtgat cggcgcgtac gcgattggtg cgcacgaggg gtacatctac
     3241 atccgcgcgg aatatcctct ggcggtcgca cgactcaaac gctgcatcgc cgatgccgag
     3301 acagcggggc ttctcggaga caacatcctc gggacgggat tcgacttcca tctgaaactc
     3361 aaaatgggtg cgggcgcgtt tgtgtgtggc gaggaaaccg cgttgattgc ctcaatcgag
     3421 ggaagcagag gcatgccgcg gccccggcca cctttccccg caaattccgg tttgtgggga
     3481 tgccccacga acatcaacaa tgtggaaacc cttgcgaaca tcaccacgat aatcaatcgc
     3541 gggggcacct ggtttgcggg gatcggaacg cctggcagca aaggcacaaa ggtcttcgcc
     3601 ctcaccggaa ggatcaacaa caccggcctt gtggaagtgc cgatgggtct gccgttgcga
     3661 gaggtgattt acgagatcgg tggcggcatt cccggaggca agcgcttcaa ggccgttcag
     3721 cttggcggac cttccggagg atgcgttccg gaaagcctgc tcgacacccc ggttgactat
     3781 gagtctatca agggaaccgg ggcgatcatg ggttccggcg gaatggtggt gatggacgag
     3841 agcacctgca tggtggacgt cgcccgttac ttcatgagtt ttgtccaggg tgaatcgtgc
     3901 ggcaaatgca ttccgtgtcg ggaaggcacc aagaggatgc tcgaaatcct cgaacgagtt
     3961 gccatggtga aacccgaacg gaacacggac gaccaactgc gtcgtttcga tggcgccatg
     4021 cttctggaat ccctggcgca catcgttgcc gacacatcgc tgtgcggttt ggggcagacg
     4081 gcgccgaacc cggttctcag caccttgaag tatttccgcg acgagtatga agctcacctg
     4141 tttgatagaa agtgtcccgc gggggtatgt cgcgggcttc tcacctacag gattgatacg
     4201 tccaaatgca caggatgcgg tttgtgtgcc gcgaagtgcc cgcagggcgc gattctgggc
     4261 gagaagagac aggctcattt tatcgtatca gaacgatgca taagctgcga tgcgtgccgt
     4321 caggtgtgta atttcggcgc agtgctcgca gagtagtact cgcgaatttg ttatcgcgct
     4381 tcgagggcaa cgggaattca cgacaacaga cggtgtgcgc aacgttctgc aatgttgttc
     4441 cgttgctcgg ataaccgaac catatggcgc acaggatgaa caggccttaa tctatgccta
     4501 ccgttgacct cactatcaat ggccgtgaaa taacagtcga agcgggtacg accattctgc
     4561 aggctgctcg tctggcaggc tttcagatac ccactctctg ctatctcagt ggtctttctc
     4621 cgaccggagc ctgcagggta tgcgtcgttg aagtggaagg ggcacgcacg ctcgttccgt
     4681 cgtgcgcatt tcctgtcgca gacggaatgg ttgtccggac gcatactgcc cgtgtcagag
     4741 cggcgcggac cgcgatcgtc cagcttttgc tcgcgaacca cccgcaggaa tgcctcacgt
     4801 gcgtgcgcaa cggggactgc gagctgcaga cgctcacccg cgagttgaac atccgcgaaa
     4861 tcccttacca gggggagcgg aggcttgccc cggttgattc ctcgagcccc gcgatagaac
     4921 gcgatccgga gaagtgcatt ctctgcggca ggtgcgtccg tgtctgccag gaaatccaga
     4981 atgtcggcgc catttcgttc accaagcgcg gctttcagag cgaagtcgca ccggcgtacg
     5041 acaggcaact ggacatcact tcctgcgtta attgcgggca atgcatcatc cagtgcccta
     5101 cgggcgcgct gcgagagcac tcctctgtgc gcaaagtctg ggaatggctg caggatccga
     5161 acatgcacgt ggtggcgcaa accgcacctg ctgttcgggt aggtatcggc gagatgttcg
     5221 gcgccgctcc cgggggtatt tacacgggcc aactcgtcac cgcgctttcc aatatcggtt
     5281 tcgaccgcgt gtttgacacc aactttgccg cagatctcac gattgtcgag gaagcaacgg
     5341 aactggtgcg tcgagtgaag aacgacgggc cgttgccgat gatcacgtcg tgcagccctg
     5401 ggtggatcaa gtactgtgag cattactacc cggacctgat cccgcacctg agcacctgcc
     5461 ggtcgccaca tgagatgctg ggcgcgctga tcaagagcta ttatgccgag cagtacggaa
     5521 ttttccccgg caatatcgcc gtcgtgtcca tcatgccgtg caccgcgaag aagttcgaaa
     5581 ctgaacgtcc tgagcttgag acaaacgggt tccccagcgt ggacgcagtt ttgacggttc
     5641 gcgagattgc ccgcatgata aagactgccg gcatcgactt cctgaacctt ccgccgcgtg
     5701 atttcgacaa tccgctgggt acttccacgg gggctgcgga cattttcgcg tctaccggtg
     5761 gcgtcatgga ggcggcactc cgaactgcgg cgttctacct caccggcaag gaactcgcta
     5821 acgttgactt ccgcgacgtg cgtggtcttc gtggcatcaa ggaagcgacg atcaagatcg
     5881 gtgacatgga tgtgaatgtt gccgtggcga gtggactggc caacgcgaaa gtgcttatgg
     5941 accagatccg caagggcgaa agtccttacg cgtttatcga gatcatggga tgcccgggcg
     6001 gttgcatcaa cgggggtggg atgccgcagg gttcaaacgt ggagacggtc cgcgctcgaa
     6061 tgcgggcgat ttacacgata gaccagacga aggtgtaccg ccgttcacac cagaacccgg
     6121 cgatccgcaa actgtacgag gaataccttg gcgaacccgg ttctgaacgt gcacaccacc
     6181 tgctccacac tacttacatg gcgcgcgagc gcagttaaag gaaaggcgtg aaaatcaatg
     6241 gctgaaacag tactcgtcat tgacgacgat caggacttcc tcgaagcgac cgagactctt
     6301 ctcgatgcga tggggttcaa tgtcgtcacc gcagcttcca gcagcgcggc catggaggcc
     6361 gtcgagaaag tcactcccga tgtgattctg cttgacgtga tgatggagag agctgactcg
     6421 ggcttcaccc tcgctcagcg cttcaagagg aaaccggaac tggcgaaaac accgataatc
     6481 atggtgacgg gagtcagcga gcacctgggg caccgttaca gtctggatac tcctcaggag
     6541 cgccaatgga ttcgagcaga cctgctcctc gaaaagccac tccggccgga ggatctactc
     6601 ggccacatcc gacaggtgtt ggagcaggcg catgagaaca gcgccgcgca tcactaggag
     6661 tgttgttcgg ggacggcgcc aaacgcaaac tgtcccccgg ttgcgttgag cgcctgtttc
     6721 ccagtgcccg gcgacctcac ggtcgccggg cgtttgtttc tggccttgcg ttttgttcag
     6781 gagaggagac cggcttccac gagcagcttc cgaagccttt ccctgttcgc ggcgctgaga
     6841 ggggcgagcg gttgtcggac gtgccccacg gggtacccta ccatactcat cgcttctttg
     6901 ataggacccg ggttcgtctc cagggcaagc aacgcttttg agaggaggaa caatctctgg
     6961 tgcagttcga gtgcctcttc gcgtttccct tcctcccagg cggcaacaag gcgggccatt
     7021 tcgcgcggaa agagattcgc aaccaccgat atgacgccag tccccccaac ggatagcagg
     7081 ggaagcgtca gcgcgtcgtc acctgacatc acggcgcacc ggcctcgtgt ccggattacc
     7141 acctcgctca tctgattgat gttcccgctc gcttctttga ttcccactac attatcgagg
     7201 tacgaaagtt tctcggttgt ctccggctcc atattgatgc cggtgcggct tggaatattg
     7261 taaagcacca gtgggattcc gacttcgttc agtcgctcta tgtgcgcaat gagacctgcc
     7321 tgtgacggcc ggttgtagta cgggagtaca atcagcgccc cgtcagcacc caccagcttg
     7381 gcgtgctggg tcatgtggac cgcttcccgc gtggagttcg agcccgtgcc ggggaccaca
     7441 gggatgcgcc ccgccgctgc ctccactgcc gtctcgataa cagcttcgtg ctcctcatac
     7501 gtcagcgtcg gcgactcgcc agtggttcct acgggaacca gtccatcggt gccctgctcg
     7561 atgtgccatt cgacaagttc ccgcaagagc gcgtggttaa cgcggtcatc ggacgtaaac
     7621 ggggtaacca gagcaacaaa agatccacgt agctgcaaca ttttcgtcac cttttcgaca
     7681 tggagcacac gggcccgtct gcacacggac gccgtcccgg aatgctcatc aactcagctc
     7741 aggccgccgg cacacggcat tacagcttgt gtagtatagg gcatgcaggg aaccacgtca
     7801 acgcgtcctg ggcttacaac aaaaaaccgg cggttttgcc cgccggtttg caaatgcggc
     7861 atagtgccgt gtgtcatgct tcgcgcatcc accagctctc aaaaagaagt tctgccttcg
     7921 cgcatcgccg gcgcgccttc gatgttacgc ggccgatgcg acgacgcgat gtactgcttt
     7981 tccccacccg gggcttgagg atctgtttac cttccattct gccgatattc aggtcgcctc
     8041 tgctcacggg aacctcctgt cgggtggatt tcacttcctt cctgcttcct ctctggaagc
     8101 cccagaactc gaccgcagcg tttttgggtc cttggtaacg ccgttgctct gtaccgaccc
     8161 aatctttgta actcaatcag cagacggaag gttcccgccc agaactgcat tgcaatggat
     8221 tcccctgtcc cgcaacgagt tgcctcaaaa ccggtaatca acctctttga ggtacccgca
     8281 ttcgtgtgcc gccttcatga acaagagaat cccacggagc tggtaactga gaagttgaca
     8341 attcggccac acgagctccg gcatgaggca gattccgtct tcgaattcca cgaactgccc
     8401 cgggatcctc atgtggtcga cctgcagtcc ccggtcgagg caatattcca gcattctcat
     8461 gcctctgcga acgaggtctt tgtcccgggt aagttcccat gcgcaaccga ttgcacctaa
     8521 cgtcaacgtg gccgcctgtt gcatagggaa gttctgttcg gggccgttca catacgtggg
     8581 gatgccttca gggcttgtcg cgcgcctgtc gctgatactc cgagtcactt ccagacacag
     8641 gttctttgcg atctcattcc gggaaatctt gaacacatgg tatgcgccta tggccaccgt
     8701 tgcgctgtga aacgcattca cgctggacgc accgggggtt tcgccgaaac cctgtgtgag
     8761 gccttcgtgt tcggcgcaat acgtatgaag ctgttccacc atccactgca tcaattccag
     8821 gagttcgtcc cgattcgtca cttcccagat cgcggcgagc tggataaggc cccaccccca
     8881 agttctcacg tacggcggca ggcggagttc ccggtctttg ttcctcaaga accaatctgc
     8941 catctgattc agcacgcgct ccacgcgagg atcacccgtc atgaagtacc actctttgaa
     9001 cccctccgcc cagttgtggc tcggcgttgt gccgccattt atgacatgtc gccttgtgtg
     9061 cgcaatcagg gcgccctcat gtcgagggtc agcatgtcgc tccgcaaagt ccacatccat
     9121 ccagtgaaga atgctgtcgt gaaaatcctt gaagtaggcg aactgccccg tacgcgcaaa
     9181 ctgacagaac gcgtggtacg ggtggtcgcc ttcgagattg ttccatacca cgtcctctcc
     9241 gccgcgtttc tggttggtgt agaggggaga gtaatcatcg ccccagttca gtttgccgaa
     9301 cccacgaatc cggttctgga agagatcgaa caggttggtc tccatccgcg gatacgtctc
     9361 cggcatgtac ggcaggtact cagagagtgt gcctgcgcgc gccctgactt caaacggcac
     9421 ctgaaccagg ggcggctttc cgaatgagta caccagtcca cgggcaggaa aacactcccg
     9481 atcttccgga ttcagcaacg ccacgagaat ctcgtgtgtc tttcccacgc caagatggag
     9541 ctgcattggc ggggcccaac gcggaaacaa ttcgatttcg atggcatctg aatcaaatgc
     9601 caccccggcc gggtagttct cccagaaatt gcgaatcgcc agcacgatct ctcccgattg
     9661 gcctacccgt gagaacccgg tttcaccata cccgatcaaa cgactcgccc gacgcatctt
     9721 ttcctcgttg ccgtgaaaaa cgctggtcgc aagcttgtcc gttcgcacgg cacacggaag
     9781 ttccacttcc ctttcggctg gcccgttgcg cacctctacc ttcacgctcg ggtcgtattc
     9841 gccacccaat ctttcaagtt ttgcgcgcag gtggtcaaca gcgaccggtt cacgattggt
     9901 gcgattcgtc gcgatgacgt caagagccag aaccggggtg cgggaaaaga tctggataac
     9961 caggtccacg tccagaagcc gcgtggtgcc attccagatg gcacctgcaa catccagccg
    10021 caatcggacg ggaccagagt ccgtggcgga tagcgtgtca attcggaatg atccaaccag
    10081 ggaatggttg aatccctcca ccgtgagtgc cccatcgacc gacaagcgcc agccctgccc
    10141 acccctctct atgacaaggg tatctccttg cctttcgaca acgatgctcg cccattcgga
    10201 ccgcgtttca ttccagccga cggtttcgga ggcttccggt ccaccgcttt ccagactgaa
    10261 tgcctcccgt ttctggcctg gaacgggagg ctctgccgta acgagcagac ggcgcacgga
    10321 gccatcgggg tggattgcaa gggatttcga ttgaacgggg aagagcttgc cggaagagtc
    10381 tcgcagaacc agcggagcac ccggcaacag agtgccggcc gccaacggta ctccgaaggt
    10441 gaccgggaag gatggaaggg gccaacggtt cacattttcc acgaacaggg gacagatttc
    10501 catcgcctcc ctccggtttt cacacagtca cggtctcaat cctgtctttt cgcgcacctc
    10561 atccatggtc tcgcgcgccg ttcgtctgca gcgttccttt cccgccgcga ggacgtccag
    10621 cacctcgccc ggacgcgcct ccagttctga atatcggctt ctgatcgggt cgagaacgtt
    10681 gttcaggttc tgcgcgagat gttttttgca ttcgatgcac ccgatctccg ccttccggca
    10741 tgcgcggtcg atctcgagaa cgagttcttt cggagacacc attttgtgaa gggtgaagat
    10801 gttacatatg tccgggtttc ccggatcgct tttgcgaagc cgtgcgggat ccgtatatgc
    10861 cggcctcagt ttttcccaca cctgttcagg agtttctgtg agcccgatgt agttgttcag
    10921 gctcttgctc attttgctct tcccgtcaag gccgagaatc cgcggcgctt ccgtcataag
    10981 ctgcttcggc tccgggaagg tttcgccgaa ccgcgtgttg aacttcctgg cgagttccct
    11041 gctcagctcg aggtgctgca attgatcttc acccacgggc accgcctccg ctttgtaaat
    11101 gagtatgtcc gcggcttgca gcacagggta ggcgaaaagc ccgaggttga tgttgtcggc
    11161 attcttctcg ctcttttcct tgaactgggt catgttctga agcctgccca tcggagcaag
    11221 agtcgagaga acccacatga gttccgtgtg ttccggcacg tccgattgga cgaaaagcga
    11281 gcacttcgcc gggtcgaggc cgcaggcgat aagaagcgct gaaacctcca acacgcggcg
    11341 acgcatcgta tcggaatcgt actctactgt catggcgtgg taatcaacga cgcaatagat
    11401 ggcgtcccac tgatcctgca accgcaccca gttcgttatt gcgccgaaat agttgccgag
    11461 atgaagctcg ccagtgggct ggatgccact gaataaccgc ttatgcctca tgagaaaacc
    11521 cagtcccgtt tttggatcat tcgttttgag ccgcgcacgt gcaccggctt gcgataatca
    11581 cccacacaag cgacaggagc cgcaagcgga agctcacggc tcctccaaac cactcctgca
    11641 tcacatccgt ccgcgcggag aaatcacaat gctatgcgga cgctttttgc acaaaaacgc
    11701 ctcaactgcc ggttacctgc actgccccct ccagcattcg gattctctgc cgcgcacgcc
    11761 cgacaagctg cgattcgttg tagtcgtcca gaatcctctg caacgcagcc tgagcttcgc
    11821 tgtatttgag ggcaagctcc aggttcgtgg acgcctccaa cagggccatg ggggcgaatc
    11881 ccgatccctt gttgtcgtct acccattcga tgagaagctt cccggcgcgt tcgtattgct
    11941 tttccgcacc aaggcagtcc gcgagtccct tcgttgcgtg gaagctcgtt atttcatcat
    12001 cccatttcga tttctcgtag attgcccgtg caccagcgta gtcccctttg ccacgcaatg
    12061 catccgcttt gtagaggcgt gccattcggt ccggcaaacc cacgaaactc ccgattatct
    12121 ggtcggcctg ggtgatggca cgatcgtact gctcctggcg gacgagcatc tccagtcccg
    12181 acacctgagc catcccgcgc tcagtctgcc gggcgtccca tgagcgatag atgtagtacc
    12241 cggttcccag aagaacgacg cccagaatga ctccggcgat gtaccacatg ttatcctgga
    12301 cgaccattga aacacgggaa aaccagatga gcagcttgtc ctgcttgatt tcccgccgtg
    12361 tcaatttccc gcgagctttt gttgcttgca cgcataactc cttctattgg gaatgcttct
    12421 ccaccatggt ggcaacgttg gcaaattcct gcttgagtgc gggatacaac gcccggtatc
    12481 gttcgtacca ttcctcgtac cgcgaaacgt tcgaaggaat cggttccgaa actgaaacga
    12541 tcttcaccag cgcgtcggac gcatccgcga gatcactgaa cattcccgag catacgccgc
    12601 ccataagcgc agcgccaaac gcgggacctt cgctcgcatt caccgtgacc accggcacac
    12661 cgtacacatc cgcctgcatc tgacgccaga acgcgctcct ggccccgcca cccgtagctc
    12721 gaatctccga gatgtgcaat cccagttcgc gcatgatcga gagcgaatcg cgcataccga
    12781 aggttattcc ttcgaagacg caacgcatga ggtgttccct tccgtgccgc aaactgagcc
    12841 cgacaaacgc gccgcgggca ttcgcatctc tgtgaggggt gcgttctcca ctcaggtagg
    12901 gaagaaaaac gagcccctcg ctgccaatcg gtgccttcgc tgcctcttcg gccatcacgt
    12961 cgtacgggtc ttgcccagac tggctcgccg aagcgcgttc gctgtctgca accacatcac
    13021 gaagccaccg caggctaccc cccgcggaca acatcacccc catcaagcac caccggttcg
    13081 gcacactgtg gcagttggtg tgcgcacgga ggtcgggatc aaccttgacg gtgtcagtgt
    13141 gagcaaacac gactcccgac gtgcccagac tcgccagaac gcgaccctct cgaacaatcc
    13201 cggtccccac cgcgccgcac gcgttatcgc ctccgcctcc cgcgacggga acgccctctc
    13261 gaaggccagt cattgcggcc acatcacgcg agatccggcc gcactcggcg accgactcgc
    13321 gtaccggaac gaggatatcc atcggaagat ccactttcct catcagttct tccgaccata
    13381 ccctgttcgc gacgtcgaac atcgcggttc ccgaggcgtc cgcaacctcc atgagcttct
    13441 cgccggtaag ccggaagcga acgtaatcct tgggaagaag aagcgttttg agccgccgga
    13501 aatttgccgg ttcctcatcg cgcagccaga tgactttcgg cagcgtgaac ccttccagag
    13561 gagggttgga aacaagccgc cgcatctctg catcaccacc gactctctcg gtgatccagt
    13621 agcgctgttt cgttgttctc ccgtcgcacc atagaagggc cggccggatc acttcgtcgt
    13681 gttcgtcaag gaacaccgag ctatgcatct ggccggataa tccgatgccc gcgatatcgg
    13741 cgcctttgac gcccgcttct gcaagtacgg aaccgatcgc cgtaatagta gcggtccacc
    13801 aggtcgccgg gtcctgctca gcccacccgg gcttcggaat aaggagtggg tactcaatgg
    13861 tgtgacgtcc gagcgtgtgt cccgaatcgt cgataacaag cgccttcaca cccgtggtgc
    13921 cgatatccag accgagaagc aaccccatcg gaagattcct cattgcgaaa cgttttcgcg
    13981 ttggccggag caaggaattc aaaggtacgc aggacggtcg ccgggagtca acgcgactgg
    14041 ccgggcgcgg aatggcacgg ttaaaaaagc gaacgtgtaa caaatcagga ggatccggcg
    14101 tctgaacttt caacacgttc gggcggggaa ccggaatgtc gacgaagcgt ctttgaatca
    14161 taaacaacaa acgcgacatt cttcgcacag gaaaagcacc ctggaaaccc cggcgtgctt
    14221 ccccggattc ccgttggaaa acccgacggg aatccccctt cacgaaccat cggcatgcca
    14281 aaagcatcgc cggagaagaa ggagcagcac gatgcgcagc ccattgtcat ccgccctcgc
    14341 attcctcgga tcgatcctcc tggtgaccgg cgggacggca gaaagccagg aagcgtcgtt
    14401 cagcgggcac cgcaacgcag tcaggtcggt cgcgtgcagc cccgacggac gcatgatcgc
    14461 ctccgcgagc ctcgacaaga cagtacgcgt ctggcaaacc gaccagtcaa acgcgatgcg
    14521 cgttctcggc gagttccccg gatctgtcct ctccgtcgct gtcggtaccg aaggtcacct
    14581 gctggtgctc gggtgcgccg acaacacaat tcgcgtatgc acctgcacgg acggggcagt
    14641 gctgaggacc atagtcacga catccggtcc tgtgtactcc gttgccatca gcccggatgg
    14701 ccattacgtc gcttcgagcg ggaatgacgg ctctgttcga atctggcaac ttgaagacgg
    14761 aacactggtc cgaacctttg cgggacacgc ggctcctgta tggtctgtcg cgttttctcc
    14821 cgatggcagg tacctcctgt cggcaagcag cgaccacaag gtaggggtat ggaacgtggc
    14881 cgacggtgcc ctgattcgta cgctggaagg gcacaccgcc ccggtgtttg atgtgacggt
    14941 gacgccggac gggcggtacg ttctgagcgg aggagtcgac aggacgatcc gcgtgtggaa
    15001 cctgtctact ggtacgctca tacggactat gagaggccac acggggacag tctacggagt
    15061 tgccgtgaat ccgggtggaa cctgtgttgc ttccagcagc gcggatggaa cggtgcgagt
    15121 ctgggacatt tcgtccgggc tcgtgcagcg gacgttctca aacgaaacgg cgatccgcga
    15181 cataacgttc accggcgacg gctgcaatct cgtcggtggg tgcgacgaca agaccgtgaa
    15241 gatctggtct gttcgtccat cctagattat tccgaaactc aacggtgtca cagtattatc
    15301 agccggtgtg agcggttctc tcgggaaccg ctcacgctta ttgagggttt tcttcttccg
    15361 aagaagggcg gtgcgtacct tttctgtagg atacttcgca cctgcagatg aagtttgcga
    15421 tcacatccgg gagtgtcgta acccacacct cttgtggaac gcggttcctt cgaagaaggg
    15481 aatggatgtt gaaaataacc cgagcgaaga gcgctggttt ttgtttcggt gtgagacggg
    15541 cggtgcatac ggctctcgag acctgccaga acacgcacaa gccggttcac atgctgggcg
    15601 atatcgtcca taacgaacga gtggtggcgc aattgcgtga ggcgggaatc cgggtcgtga
    15661 aaagtctgga tgaggtggat gacggtgtcc ttctcatccg ggcgcacgga gcagaacctg
    15721 caacctacga ggaagcgaag acacgtggcc tgcagatcgt cgatgccacc tgcccacttg
    15781 tgaaggagat ccacgagcga gcgcgcgaac ttcatgccgc cggttatccc cttctcgtaa
    15841 ttggcgatca cggtcatgat gaggtcaaag ggatcgccgc gcaggtgccc gacgccattg
    15901 tggtttcgac tcccgaagag gtcaggtcac tcgggcggaa gttccgtcgt atcggcgtgg
    15961 tagctcaatc aacgcaggac cttgacaatg tgcgcgcaat cgtcagcgag ctgatcccga
    16021 tctgtatcga aatccggttc gtgaacacga tctgttaccc gactgcgaag caccagatgg
    16081 aaatccgagc gatgccgctg gagaacgacg tcatgatcgt agtcggttcc tttaccagcg
    16141 caaacacgaa gcggatgacg cagatatcca gagggttgaa ccctcgaacc tatcaggtaa
    16201 cgggagcgga cgatctctgt gccgactggt tttcgggtgt caacacagtt ggcatccacg
    16261 cgggggcttc tacccctgac agtgtcgtgg aagaagtgat tgctcgaatc aggcaattgg
    16321 cagggacgcc ggtggaaatc gtatgacgtt gcggctctgt ttcaaacact ttcgcgatgt
    16381 tcgcgcagta ctgctggcgc tttccctgtg ttctctcctg aatggatacc cccgggaagc
    16441 acacgcggca gattctttcg ccggggccgc tattgcgcag ttgcagtacg gcggcggcgg
    16501 tgactggtac gcgaatcctt cctcgctccc taacttgctg aagttcgccc gaaaagaagc
    16561 cggaatatcc gtttccccca caccgggacg agttcgcata acagacccgg agctgtttgc
    16621 ctatcccctc atctacatga ccgggcacgg caacgttcgc ttcagcgacg aggaagtggt
    16681 ccggcttcgc cgatatctcc tttcaggagg tttccttcac gcggatgaca actacggtct
    16741 tgatgagagc ttccggcggg aaatgagacg ggtgttccct gacaacgcac tcgtggaact
    16801 gccttttgac catcctatct accgaagctg gttcgtgttt ccgaacggat tgcctaaaat
    16861 acacgagcac gacggcaaac ccgcgcaagg attcggcatt ttcgccggcg gccgccttgt
    16921 tgtgttctac tcatacgagt ccgatctggg tgatggatgg gaggatcagc acgtgcacaa
    16981 cgacccggag gagaaacggt tggaggccct ccgaatggga acgaacatcc tcgtgtacgc
    17041 gctcacgcat tgaccgcgcc cggtgattcc ctgccggcca catcaaattc cgagtgattc
    17101 cagcccgcga accatgtaag cctgcaaaac acccgattca tcagaattga aaaacgcggt
    17161 tttgccgtcg ggcgacagga acgggtggat atgggcgtcc ttcctccacg agcaacgggg
    17221 ataggcgata cacgtccatg aagagagggg gtccgttcct gcgttcccca ttctggcgac
    17281 gaaaacccgt cctccgttat cgcacgcaaa tgtgtcggta acgaaccatg agccgctggc
    17341 gtcggttgcg aaatgggaaa acgaaggcga ggggaactcc cggctgaggt tgttcctgat
    17401 tgcccccggt gaacgcgcgc cgtcatggcc cgtgtgtggc acacaaacgc tctcgataag
    17461 ctgttgctcg tccggatggc gcgtatctgt gctcgtgatc gcccaggcgg tgtttccccg
    17521 ccagcactgg tgcccctggc agaactcgtt cccatcccgt ccccacggca tgttacggaa
    17581 gctggatccg tcatctcgga tcacgtgtac atccgccccg tttcctcccg tcaggtcaat
    17641 gatttcccct tcaggcgtgt gcgtgttacc gtggttctcc tgaaccagaa tgtcgcgctg
    17701 agacccggga tgaatcgatc ggctgtactg gggatgtacg ttgcaccagc tgggtccttc
    17761 cagaatcaga gtcacggaag ggctttccag atcgaatacc atcaaccccc acgtccttct
    17821 gccggaaccc atgtcagcca ggaagcagga aagggcgagg cttttcccgt ctgaacgaat
    17881 ggtcgacagg ggataaatct ggctgggaaa gaacggcgta cctggtacgg ttccttgcaa
    17941 cacgtagagc acttcccgcg cacttccgtc gagattcatt cgtttcagcg tcaatttccc
    18001 accgtcgagg cgggtttcgt cgacgaaata gtacacgtgc gtgccgtctg gcgttactga
    18061 aggcgctgtc gcacccgttt cctgcgttac cggcgacagt tcgcaacctt gctcaa
//
LOCUS       DAOTNG010000017        17947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3154_length_17947_cov_5.197407, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000017 DAOTNG010000000
VERSION     DAOTNG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17947
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3154_length_17947_cov_5.197407"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="P5179_01675"
     CDS             complement(<1..657)
                     /locus_tag="P5179_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRS93962.1"
                     /translation="MAALTLRDIDVRGKRVLVREDYNVPLDENQNITDETRIQATLET
                     VRWIIDRGGKLILMAHLGRPKGKPDPKQSLAPVAKRLAEILGKPVVLAPDCVGEETEA
                     VVGRMKDGDVVLLENLRFHKEEEANDPEFAAKLAKLGDVYVNDAFGTAHRAHASTVGV
                     TAHFKQNAAGFLMEKELKYLGEALEKPARPFVAIMGGSKISGKIDVINNLLPKVDALL
                     I"
     gene            complement(761..1759)
                     /gene="gap"
                     /locus_tag="P5179_01680"
     CDS             complement(761..1759)
                     /gene="gap"
                     /locus_tag="P5179_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRS93963.1"
                     /translation="MAIKLGINGFGRIGRLVFRAAVAQGGVDTLLINDLTSAATLAHL
                     LKYDSIHGRFPGDVSSEGENIVVNGKKIPVSKEKDPSKLPWKALGVDLVLESTGRFTD
                     AEQLKMHLDAGAPRVILSAPAKGKLDGTIVVGVNDKTLDKSWKLLSNASCTTNSLAPV
                     AKILNDTFGIVSGMMTTVHAYTNDQVILDFNHSDLRRARAGAMNIIPTTTGAARAIGE
                     VIPELKGKLDGQALRVPVPDGSMTDLTVVVGKATTKEEVNAVVKAAAEGPMKGILEYT
                     EDPIVSSDIVGNPHSSIFDAKCTMVLGGTLVKVCMWYDNEWGFSNRVVDLAKMWVS"
     gene            complement(1826..2866)
                     /locus_tag="P5179_01685"
     CDS             complement(1826..2866)
                     /locus_tag="P5179_01685"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRS93964.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAVTVSAIDSGYEVLGFLRGWAGM
                     LEKDYEILTKERVYDIHRQGGTILYSSRTNVAKVKDGFEIVKRNLADLNVYCLVAAGG
                     EDTLGVAKKLADAGGNVVGVPKTIDNDVNATDYTFGFDTAINRVMEALDRLHTTTEAH
                     QRIMVVEIMGRHAGWMALHGGMAGGAHYIAIPEVPFDTDEIEKLCRERYAAGKKYAMV
                     AIAEGAEDPKLTAKLQRSAEKDSFGHIRLGSGRGIAEVVAEELQERTGIETRHIQLGH
                     LQRGGDPTAFDRVLGTRLGVKVVDMINAGEFGKMASLRGGEMVAVPLADAVGELKTVP
                     TDRLDVAKKFFG"
     gene            complement(3238..5682)
                     /locus_tag="P5179_01690"
     CDS             complement(3238..5682)
                     /locus_tag="P5179_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRS93965.1"
                     /translation="MRTKVTALILLTIILVCSSHVRGQDDEATSLVNHAAVQLANGDS
                     DSAEVLYRTALRINPRFALAAVGLGRILVSKDSIDAAKRLFEQAESWDRNKGYKQFGE
                     GLILKKRGQRDRAKTRFQEAFRKNNRFADALLEVARIQASGFVDRFAAKRTYRRVLEV
                     APNHPSAAYELGRLQEDSGELDEAIISYEAQLRANPQHGETMIRLGYTLLDKQHYWHA
                     RQRLYDALLNTSGKEGELSLALAATYMGDRQFARAHEAYMQALQLLPEDERALYEDIS
                     LITSPQEAAYVQRVSGEQKAIFLQKFWLRRDPTPATAVNERMLEHFRRVWYAKRHFSR
                     GRQPWDDRGQVYIRYGEPDQRASSSNPNFSTNHNVDMVRERYMNALYGTNPPESLQRG
                     VLPVFPLVDPVQMLQSGFESGQPTQSTSTTGQQYGDVGETGDTGSDEAAASAVTSTQQ
                     AREQVPTFSAMAQAQLIRWEEWTYTGVLNGLNLTFVDRIGRGDFRFATPPPTTDMNKA
                     TVMQQYAPQEQLTIAAMEVPEQFEYDAQQAPFNFYYYLAQFREPTTSKTEVDIYYGLP
                     MADLQFRAQDDGTFKATVQNGFAVFDTLWNVQGRFLDRTDLISPTRPSQDRGSIHVDT
                     RSMVLDGGQRVLLSVQAEDVGSGRLQAYRENVPIAKFDSSTLAMSDIVVAGSIRPADS
                     TSARKFVRNGLHILPMASRSFRRGQPIHVYFEIYNLTRGTDYGDTEYEVEHAFRTGAG
                     EGGSILGSIGRILGGSPRRVGVARVMEGIRGSEYQHFQLSTSGIPKGEYTLIITVRDL
                     KSNQRVTKERLVMIGD"
     gene            complement(5743..6531)
                     /locus_tag="P5179_01695"
     CDS             complement(5743..6531)
                     /locus_tag="P5179_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93966.1"
                     /translation="MRSTRLCIRALLFLPRVRPRKNAATYSKRRLYAVAPSVATLAFV
                     IQCVDGCENRPFASRSPLPLYGDFRAYSIVDERRTASSPLDTISVYLFGDGVSDNAAV
                     TVNGTRVPRVLTAGNQALFGNSIPLQPAEILQVDVSTTWRSVRNVIGLPASRPVMVSP
                     AAGSYLNRNEPIVVRWSGITGGGVGLQLHRLSANGALGDTLWTTTAAAEDDSVVVPRN
                     VLSAVNDTTLLLSLWHESVGQGEGFTGVFYMSAGFGIRRLVRFN"
     gene            6666..8123
                     /gene="dacB"
                     /locus_tag="P5179_01700"
     CDS             6666..8123
                     /gene="dacB"
                     /locus_tag="P5179_01700"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRS93967.1"
                     /translation="MRKGSKFFLAGCISIGLFATCIVDAFATETPWRCIAASLDSIVQ
                     RRALASAQVGIAVWSFDRREWVYEKNADRLFVPASNAKVLIGAAALHTLGPAKTFATT
                     LTADRVPISGDSVLTGNIYLYGGGNPDLVTGDLTGFAETLRASGVRRIRGAVVVDASH
                     FDSVNYALGWSWEDRPYPYCPPISAFLLNGNVVQVTVRPGSAPGRPVHVTLVPKDAGI
                     PVSVAARTGSEHNTSSRIRVERSNSGIVVKGTMGGRAEPITVWRTVPDPVRYAGSVFM
                     GALQDAGITVERGMSIGTSPRNLPVLARVYSDPLDVILRRYLKRSDNLLGETLVKQMG
                     VATSGRGTWEDGLSAVRRELRDIAGLDSNAYRLADGSGLSRLNEVSPRMLVQVITSAS
                     SSFSLAPEFLSALSTAGVDGTLSHRMTEDETRAYLRGKTGTLSGVSSLSGVVESRSGA
                     RFACAIMMNGTSPSAQTMRQAQDDVVRVLRNCIEQ"
     gene            8406..14453
                     /locus_tag="P5179_01705"
     CDS             8406..14453
                     /locus_tag="P5179_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HRS93968.1"
                     /translation="MTASDKWTERALRFLLSCVLFCWTQSAFAQRETGAPGPHADTTG
                     AQIVRADSSVAGNVSGFWPRATYLVTDGLTVSAGDTLVIEAGATILFQGPKDREKLSI
                     NCLGRLVVAGQDTVPVTFASAIPEGGAGRIWGGILYVGNMADGRVENAIFRNAEIGIQ
                     VRRGIIADRPAEADSANPIPEYILRRPTGPVVIRNCTIDSASFNGLVFVGVDSSVVAE
                     NNLIQNCSSGISCEEGARPILRLNRILNNTSTGIICSSESSPLITGNTVVGSSTAGII
                     CANNSNPTIDGCIIAKNGIGVSVTKSNPMITRCTIAKNEFSGIIAYEGAAPIVSSCNI
                     RGNGLSGIDNRSSGNITAERVWWGFITTDQRKAPVLDGGRRRERGEPVPEGQTGSVRI
                     ANSLSSPSLDAPGTPKQAQELILARDNAITNRFPERGEVSNGDTVWIQVRAIDESPYL
                     EDEAIIVFRSSSGGAAGTQRVLTESSASSGIYKGYVIASLAEGDPNIVVQVRNGDVIT
                     VETGTTPPLTARVVFVSKPPAIQNLRVDGQRRGARLTNPRPVFSWRYWDNENDPQRGT
                     ELELSTDSTFLTQHWKHAVSGPLSSYTFDGPTLTRGQTYFLRVRASDGYNFGRWSVTS
                     FHMNTPPPTPILASPEDGAILTDPRHKPVIAVRNVKDPDGDAVSYIFQAAYGEDFSSP
                     RLRAGGPEASQPVPEDPASDITVWRAMPTLYENTRIWWRAKATDGTEESEWSAPRSFS
                     LNTTNDPPMPFVMLQPERDSTTNSLTPRLEWSHSYDPDPGETISFVVAFGRNKDLRDA
                     VIRHVEPVNEPVQSYQITPSEMLLDNTWYYWTVRLEQDGRVLFPANTAARDTTSVWRF
                     FVDTGNDPPVVAGIPPVVMTEDTPYKMNLGRYISDPDHPTDVLKVTVTGTEHLTAALG
                     PGNELTLTPAQNWFGGPERVRILVIDPILAEANGEISVSVTPVNDPPAVARVPDQSVA
                     EDTDLRVDLKPFISDVDNTASEMRWTATADTRKLTVSFADGVATIRGTQDWFGTTPVR
                     VTATDTGGLSASSEFNVTVTPVNDPPAVVQIPSIRTKEDEAAVVQLDNFVTDPDNQKS
                     ELRWSAVAEEPLTVTIDPATRRASISAPPNWAGAEHKVTFTVADPDGLSDRIVVAVGV
                     EAVNDPPTIAPIPDQRFNEDATLTLDLDPFVSDPDNTAREMTWTAVSGGNIKAAVDAA
                     TRRVTFSAARNWYGGPETIRLTATDPGGLRATQSVNVSVISVPEAPVFARIPAVNIDE
                     DGQFVLALDEYLSDPDHRNDQLTVSFVAPQNVKVEFDRRTRRLTLSAPPNWNGGPETV
                     ALEAVDPDNERASATFSVTVRPVNDPPVLRAIPPVAFREDESTTLRLTEFVTDPDNTP
                     EQMQWVISGQARLKVQIAQGVATFSADKDWNGTETLTLKATDPGGLFAQTQIRVTVTT
                     VNDAPVVRQLPPVAFDEDGSSTVRLFDFVSDVDNTNEQLTWEVSGNNRVKVAIARGTA
                     TFTAEPNWHGTENLTITVADPDGLRASSSFTATVRSVNDPPVIARIPPVSFEEDGRTT
                     VDLAPFGSDADGDALTWTAASANPNLRVAVSGSVLTLSATPDWNGGPINVTVTATDPS
                     RGQATTTIPVTVTAVNDPPTLKALVPVTFPEDGTATLNLLNQVTDPDNTPAQMRWEAS
                     GGTNVKVRVAQGVATLTAEPNWHGSETLTITATDPGGLAARGTLSVAVSSVNDPPVIS
                     RLSAVSFDEDGSTTLDLAPFGSDADGDVITWTATSANPNVRVAVSGSMLTISAAPDWS
                     GGPVNVTVTATDPAGAKATATIPVTVRAVNDPPELRELPSVNFNEDSTATLRLAQYVS
                     DKDNTPAQMNWQASGNTNVKVRFAQNVATFSAERDWSGSESVTITVSDPQGGSASRDV
                     RVTVRPVNDRPRVQPIPDATVEAGGQTEIDLAPFASDPDGDPLTWQVAGQTGRITPVV
                     TGSTLRITAAAGAAGVATVNLVVRDPAGAQVTARVRITIQAAAPVQAPSPSGGGTTP"
     gene            14490..15362
                     /locus_tag="P5179_01710"
     CDS             14490..15362
                     /locus_tag="P5179_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93969.1"
                     /translation="MWWILFSIFVAPVLFLFLLVAVGALMPMRVGVSVQGQAQSAKEW
                     GGRATFGVRFFGGLIGLGFRAEGEASTRTHLAGGVLFWRWFFPLKSLDSIFARAPEVR
                     EVLSAPASATREPGVALVQPPPEKGPAASTRVEKDVETGGPADWTRLFEDSDLVDFPM
                     DWQRTSRPETTTAGVLADLRAAIARVWPFVREARRRFKGVVTLRFFRLTGSFGTGDPA
                     GTAWLLGTAYALAGAKGSADSVRLAGDFQRAIADGRCEVEWKVSTIRLWAAALALAWL
                     NWKHARALRTKKEA"
     gene            15377..15730
                     /locus_tag="P5179_01715"
     CDS             15377..15730
                     /locus_tag="P5179_01715"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HRS93970.1"
                     /translation="MQTLLERLRDLVQTETVIGKPIEAGGVIIIPVSRVSVGFGAGGN
                     MNAQGAAAGAMKGGGGGGGVRVEPLGFVVISNGKAEVLPMKPDEPAINKIVDMVPGIW
                     DSVRSMLDKRDRKKD"
     gene            complement(15838..17520)
                     /locus_tag="P5179_01720"
     CDS             complement(15838..17520)
                     /locus_tag="P5179_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93971.1"
                     /translation="MPAPTALAIPAIICYSVSPTPYLNEHAANVKGLYDGFFFQCGSW
                     DSGVLTNIGVGNTPATNPNWLRLAGQNVTSLKSVGCTENLLAISFGDSEPWPSPETLL
                     SGEFTEKLRQHFSALGKAAKATGFRGISIDTEYPYPRYSLDHPIYTYAGYTPGDLMEA
                     SYRQGRIVMSAILDEFPDAVIFNLPGSLEGRPLARQFVKGLIEEMANRNAPGGFHFGT
                     EYSYTLHEPVTQAAIPRHEDAKMDRFLSGTSLEYWRSTCTMAPGVWPCHMVETGADDY
                     PVRPWDEEMAELRQQMRILRSTTKRYMWSFSGQPIWLLPSAENRDRYGLSATYPGAEV
                     FIPIWHDILRERVRYDQTPGADPRMIRLFDATKRFDEGRIDATEFCNAFGTPAKWWAL
                     GVLSNPFVNPARTAKEALSMPISPSLVFFGRDGAVRWFPWAVQDNRGIVSMRTFTDYL
                     MTDNSSVHLATYVHSPRAVDAWLNLGWDDGLVVQVGDSVVFDRAEYPERGHGAMYLDR
                     YQFEAKVPIHIPAGVTRINVTTINAKGGWMYTFRITDADGYPLPDLKFSLED"
BASE COUNT         3667 a         5266 c         5303 g         3711 t
ORIGIN      
        1 gatgagaagc gcgtccacct ttggcaagag gttgttgatc acgtcaatct tcccactgat
       61 cttggacccg cccatgattg ccacaaaggg ccgcgcaggt ttttccaacg cctcgccaag
      121 gtatttgagt tccttttcca tcaagaagcc tgccgcgttc tgcttgaaat gcgcagtgac
      181 gcccaccgtt gacgcatgcg cacggtgagc ggtgccgaac gcatcattca cgtacacatc
      241 gcccagcttc gcgagttttg cggcaaactc gggatcattc gcctcctctt ccttgtggaa
      301 acggaggttt tccagcaata cgacatcgcc gtctttcatt cgtcccacaa cagcttccgt
      361 ttcctcgccc acgcagtctg gcgccagcac aaccggtttg cccagaatct ccgccaacct
      421 tttcgcaacc ggcgcaaggc tctgttttgg atcgggcttg cctttggggc gtccaaggtg
      481 cgccataagg ataagcttgc caccgcggtc gatgatccag cggaccgttt ccagagttgc
      541 ctgaatccgt gtttcgtcgg tgatgttctg gttttcgtcc aacggaacgt tgtagtcctc
      601 gcggacgagg acacgcttcc ctcgaacgtc tatgtcacga agagtgagtg ccgccattga
      661 gtggtctccc cgatggatgc tggttttccc gaaacacgag gggcgcaccc cggtgcgccc
      721 ctcacatgat acactctgtt acgcatttcg tgtgcggggc ttacgacacc cacattttcg
      781 cgaggtcaac cacgcggttc gagaagcccc attcgttgtc gtaccacatg cacactttga
      841 caagcgttcc gccaagaacc atggtgcact tcgcgtcaaa gatgctgctg tgagggttcc
      901 cgacgatatc gctgctgacg atcgggtcct cggtgtattc gagaatgccc ttcatcgggc
      961 cttccgctgc cgctttcacg accgcgttca cttcttcctt tgtggtcgct ttgcccacga
     1021 caacggtgag gtccgtcatt gatccgtccg gcaccggaac gcggagcgcc tgaccatcca
     1081 acttgccttt gagttcggga atcacctcgc cgattgcgcg ggcggcgccc gtagtcgtcg
     1141 gaatgatgtt catggcgccg gcgcgggccc gacgaaggtc gctgtggttg aagtcaagga
     1201 tgacctggtc gttggtgtac gcatggacgg tcgtcatcat tccgctgacg atcccgaacg
     1261 tatcgttcag gattttcgcc accggagcga gcgagttcgt cgtgcacgat gcgttcgaaa
     1321 ggagcttcca ggacttgtcc aatgtcttgt cgttcacgcc gacaacgatg gtgccgtcaa
     1381 gcttcccttt cgcaggggcg ctcaggatga cacgcggtgc accggcgtcg aggtgcatct
     1441 tgagctgttc cgcgtcggtg aaacggccgg tggattccaa cacgaggtcc acgccaagcg
     1501 ccttccacgg caatttggag ggatcctttt ccttgctgac cggaatcttc ttgccgttga
     1561 cgacgatgtt ttcgccttcg gatgagacgt cgcccgggaa cctgccgtgg atagaatcgt
     1621 acttgagaag gtgagcaaga gtcgccgcgc ttgtcagatc gttgatcagg agtgtgtcca
     1681 ccccgccttg cgccaccgca gcgcgaaaca ccaagcgccc aatgcgcccg aacccgttga
     1741 tacccagttt gatcgccata gtatcctcca aatttgcgtg tgagcccgaa atacaaacct
     1801 tgagagcgcc cgacaggcag tgtgatcagc cgaagaattt cttcgccaca tcgagcctgt
     1861 cagtcggaac cgttttcaat tcacccactg catccgcaag cggcacggct accatctcgc
     1921 cgccgcgcaa tgatgccatc ttgccgaact cgccagcgtt aatcatgtct acgactttga
     1981 caccgagtcg cgttcccagg acacggtcga acgcagtggg atcgccgcca cgctggagat
     2041 ggccgagctg gatgtggcga gtttcaatcc cggtgcgttc ctgcagttct tccgcaacta
     2101 cttcggcgat accacgcccg cttccgaggc ggatgtgacc gaatgagtcc ttttccgcgg
     2161 acctctgaag cttggcggtg agcttgggat cttcagcccc ttcggcgatt gcgaccatgg
     2221 cgtatttctt cccggccgcg taccgttccc tgcacagttt ctcgatctcg tccgtatcga
     2281 atgggacctc gggaatcgcg atgtagtggg ctccgccggc cattccaccg tgaagagcca
     2341 tccatcctgc gtggcggccc atgatttcaa caaccataat gcgctggtgc gcttcggttg
     2401 tagtgtgaag acggtcgagc gcttccatca cgcgattgat tgccgtatca aacccgaacg
     2461 tgtaatcggt cgcgttcacg tcgttgtcga tggtcttggg aactcccacg acgtttccac
     2521 cggcgtccgc gagtttcttc gccacgccga gcgtatcttc gccaccggcg gcaacgaggc
     2581 agtagacgtt gagatcggcg agattgcgct tcacgatctc aaacccatct ttcaccttgg
     2641 ccacgttcgt ccgcgacgag tagaggatgg ttcctccctg acggtggata tcgtacacac
     2701 gttctttcgt aaggatttcg taatcctttt cgagcatccc cgcccagcca cgcaggaagc
     2761 cgagtacctc atatccggag tcaattgcac tcaccgtcac tgctctgatt accgcattca
     2821 ggcccggcgc atctcctccg ccggtcagga ttccaattcg cttcatctgc ttctctccgc
     2881 tgtccttcgg tggggtgtgg cgcgcgggtg tgaggattct accgtgcaac ctcggcaaag
     2941 acccggcgtg taccgtgaaa aggcggtttg ccgccgccgt agaaatgcct cagatcagag
     3001 ttttaaggta cagcgagcgc ggaaatgggt caaggcggcg acgcgaagga ttagtacgct
     3061 gagatcctgt ggtaccttct cctcgacttt aacgtttgat gggattcagc gcgtcatgtg
     3121 gatgactgaa gtccagagtt accggtacgt tttgccaact ggtgtttcag gatcgtgtaa
     3181 cggaacgcgc caggtttcca aggggcacta ctgaattcat ctggaatgca gtcttgatca
     3241 atccccaatc atcaccagcc gttctttcgt cacgcgctga ttgctcttca aatctcttac
     3301 ggtgattatc agggtgtatt cgcctttcgg tataccggag gtagacagtt ggaaatgttg
     3361 gtactcggat ccgcgaatcc cctccatgac gcgtgctaca cccacccggc gggggctgcc
     3421 accgagtatc ctaccgatgc tgccaagaat gctgccacct tcgccggcac cggtgcggaa
     3481 cgcgtgctcc acttcgtatt ctgtgtcgcc gtagtccgtg ccacgtgtga gattgtagat
     3541 ttcaaaatac acgtggatag gttgccctcg tctgaacgag cgagaagcca tcgggagaat
     3601 gtgcagcccg tttcggacga actttcgcgc gctggtgcta tcagcggggc ggatggaacc
     3661 tgccaccacg atgtcactca tagccagagt tgagctgtcg aatttcgcga tcggcacgtt
     3721 ttcgcggtac gcctggagtc gtcccgagcc gacgtcctcc gcctgcacgg aaaggagcac
     3781 gcgctgcccg ccgtcgagca ccattgatcg ggtgtccacg tggatgctac ctctgtcctg
     3841 gctggggcgg gtcggcgaga tcaggtccgt tcggtcaaga aaacggccct gcacattcca
     3901 caacgtgtcg aaaacggcaa acccgttctg tacggtcgct ttgaaggttc cgtcgtcctg
     3961 ggcacggaat tgcagatccg ccatagggag cccgtaataa atgtcaacct cggttttgct
     4021 tgttgtgggc tcccgaaact gcgcgaggta gtagtagaaa ttgaaaggag cctgctgcgc
     4081 gtcatactcg aattgttccg gtacctccat cgcggcgatg gttaattgct cctgcggtgc
     4141 gtattgctgc atcacagtgg ccttattcat gtcagttgtc ggcggaggcg tggcaaaccg
     4201 gaaatccccg cggccgattc ggtccacgaa cgtgagattg agcccattca gaactccggt
     4261 gtacgtccat tcctcccacc tgatgagctg tgcctgcgcc atggcgctga acgtggggac
     4321 ctgctccctc gcctgttgcg ttgacgtcac agcggatgca gccgcttcat cgctgcctgt
     4381 gtcgccggtt tcgcccacat ctccgtactg ctgaccggtt gttgacgtag actgcgtcgg
     4441 ttggcccgac tcgaaacccg attgcagcat ctgaacgggg tccacgagag gaaacaccgg
     4501 cagaacaccc cgttgaagac tttccggagg gtttgtcccg tagagcgcgt tcatgtatcg
     4561 ctcgcgaacc atatctacgt tgtggttcgt gctgaagttg gggttacttg atgaagcccg
     4621 ctggtcgggt tctccgtaac ggatgtacac ctgtcccctg tcgtcccaag gctgccttcc
     4681 ccgcgagaaa tgacgttttg cataccacac gcgacggaaa tgctccagca ttcgttcgtt
     4741 caccgcggtt gccggcgtcg gatcgcgtct gagccagaac ttctgaagga aaatggcctt
     4801 ctgttcgccg gaaacgcgtt gcacatacgc agcttcctga gggctcgtga tgaggctgat
     4861 atcctcgtaa agagcgcgtt cgtcttccgg aagaagctga agagcctgca tgtacgcctc
     4921 gtgtgcccgg gcaaattgcc tgtcgcccat gtaagttgca gcaagcgcca gactgagttc
     4981 cccctctttg ccggacgtgt tgagcaacgc gtcgtaaaga cgctgtcgtg catgccagta
     5041 atgctgtttg tccagaagcg tgtaccccag ccggatcatg gtttccccgt gctggggatt
     5101 cgcacgtaac tgggcttcat agctgatgat cgcctcgtcc agttcccccg aatcctcctg
     5161 aaggcgcccc agttcgtagg cagcggaagg atgattgggg gccacttcca gcacgcgcct
     5221 gtatgttcgc ttcgccgcaa acctgtcgac gaaacctgat gcctggatgc gcgcgacctc
     5281 aagcaaggca tcagcaaacc ggttgttttt gcgaaacgct tcctggaagc gcgtcttcgc
     5341 ccggtctctc tgcccgcgtt ttttgagaat caggccttcg ccaaactgct tgtaaccctt
     5401 gttgcggtcc cagctttccg cctgttcaaa caagcgtttc gctgcatcga tgctatcttt
     5461 ggaaaccagg atacggccaa gcccaaccgc cgcaagcgcg aaacggggat tgatgcggag
     5521 tgcggttcgg tacaggacct ccgcactgtc agagtctccg ttcgccaact ggacggcagc
     5581 gtgatttacg agggaggtcg cttcgtcgtc ctggccccgg acgtgcgaag aacacaccag
     5641 gatgatcgtg agcaagatca acgcggtaac tttcgttctc atccagaaaa tctcccggat
     5701 cgacgtttca cacccaccag tttcgagaac acccagacac actcagttga agcgcaccag
     5761 ccgccgaatc ccgaatcccg ccgacatata gaagacaccg gtgaagcctt ctccctgccc
     5821 cacagactcg tgccaaaggc tgagaagcaa ggtcgtgtcg ttgaccgcac tcaacacgtt
     5881 ccgcggtaca acaacggaat cgtcctctgc ggcggctgta gtggtccaga gtgtgtcacc
     5941 gagcgcgcca tttgcggaaa gcctgtgcag ttgcagaccg acgcctcccc cggtgatccc
     6001 agaccaacgc accacaatgg gttcattacg attcagatag cttcccgctg ccggagaaac
     6061 catgacggga cgagacgcgg gcaaccctat cacgtttcga acgctgcgcc acgtcgtcga
     6121 tacatccacc tgaaggattt ccgccggttg cagtggaatc gaatttccga agagggcctg
     6181 attccccgcg gtcaacaccc ggggaactct tgttccgttc acggttactg cagcattgtc
     6241 ggacactccg tcgccaaaca agtacacgct tatggtatcc aacggtgagc tggcagtccg
     6301 ccgttcgtca acaatactgt acgcacggaa atccccgtac agcgggagag gactccgcga
     6361 cgcaaacggg cgattctcgc acccatcgac gcactggatg acaaatgcca gcgtcgcaac
     6421 cgacggtgcg actgcgtaca ggcgccgttt cgaatatgtt gcggcgttct tcctcggccg
     6481 aacacggggc aggaagagaa gagcgcgtat gcacagtctc gtggaacgca tggtatgaat
     6541 aacgtgccaa atcagcgaaa accccaagga gtgcgatacg gggttgcccc acacgtcctc
     6601 gggattatac cctcgtagac gacttcaggt ttcctgagaa cacggatttg agggcttcag
     6661 ataacatgcg aaaaggatca aagttcttcc ttgcaggttg cataagtatt ggattgttcg
     6721 cgacctgcat cgtggacgcc ttcgctaccg aaacgccatg gcgttgcatt gcggcgtcac
     6781 tggactccat cgtccaacgg cgggctcttg cttccgcaca ggtaggaatc gccgtctggt
     6841 catttgatcg acgtgagtgg gtgtacgaga aaaacgccga tcggctcttc gtgcctgcgt
     6901 caaacgccaa agttctcatc ggggcggctg cgctgcacac tcttggccca gcgaagacat
     6961 tcgccaccac acttacggca gatcgggttc cgatttcggg cgacagcgtg cttacgggca
     7021 acatttacct gtacggcggc ggaaatccgg acctcgtaac tggtgatctc accggattcg
     7081 cggagacatt gcgcgcctcc ggagtacggc ggatacgagg tgctgttgtc gttgacgctt
     7141 cccattttga ctccgtgaat tatgcgttgg gatggtcgtg ggaggaccgc ccttaccctt
     7201 attgtccccc gatcagtgcc ttcctgctca atgggaacgt cgttcaggtt acggtgcgcc
     7261 cggggtcggc ccccggccga ccagttcacg tgacgctcgt gccgaaagat gcgggaatcc
     7321 ctgtttccgt cgcagccaga acaggaagcg aacacaacac gtcgtcacgg atccgtgtgg
     7381 aacggtccaa ttccgggatc gtcgtcaagg gcacgatggg cggacgcgcg gagccgataa
     7441 ctgtctggcg gacggttccg gatcccgtcc gatacgccgg ttcggtgttc atgggtgcac
     7501 ttcaggatgc cggaatcacc gtggagcgag gcatgtcgat cggcacctcg ccaaggaatc
     7561 tgccggtgct tgcgcgtgtg tactcggatc cgttggatgt gattcttcgc cgatacttga
     7621 agaggagtga taacctcctt ggcgaaacgc ttgtcaaaca aatgggtgtt gccacgagcg
     7681 ggcgtggaac ctgggaagat ggtctttccg ccgtgaggcg cgagttgcgg gatatcgccg
     7741 gtcttgattc gaacgcgtac cgactggcgg acggctccgg cctcagcagg ctcaacgaag
     7801 tatcaccgcg gatgctggtt caagtaatca cttccgccag cagttccttc agtctggctc
     7861 ccgagtttct ttccgcactt tctaccgctg gagtggacgg cacgctttca catcggatga
     7921 cggaagacga gacgcgggcg taccttcgcg gcaaaaccgg cacattgagt ggcgtcagct
     7981 cgctttccgg cgttgtcgaa agccgttccg gcgcgaggtt cgcgtgcgcg atcatgatga
     8041 atggcacctc tccatccgca cagacaatgc gccaggcgca agatgacgtt gtgcgcgttc
     8101 ttcggaattg catcgagcag tgacattccg aacgcctgcc atcctctttg ggggtcgggc
     8161 gtaatccccg gcggacggga aaagaacccc gaatttcgta ccttgtaacg tgcgcgcgtc
     8221 cgcaccgcat atggtctttg tcattttctg ccttgtactt ctattgaact tccttgacct
     8281 tagctctcgc gcgcacgaac ttactactgt tgtattttgt cccgaactca aaatgacgga
     8341 ccccttcccg cgggaaggga atgggtgcgg aatccggcgc acgttctggc agggggtact
     8401 cagggatgac tgcaagcgac aaatggacgg agcgagcgct tcgcttcctg ttgagttgcg
     8461 tccttttctg ctggacgcag tcggctttcg cacagaggga aaccggcgca ccagggcctc
     8521 acgccgatac cactggagcc cagatcgtca gggcagattc gtctgtcgcg ggcaacgttt
     8581 ctggattctg gccacgcgca acctaccttg tcaccgacgg gttgactgtt tctgcaggcg
     8641 atacgctcgt aattgaggct ggggctacga ttctgttcca ggggccgaag gacagggaaa
     8701 aactcagtat caactgcctc ggtcgactcg ttgtagcagg ccaggacacg gtcccggtca
     8761 catttgcgag tgccattccg gaaggcgggg ccgggaggat ctggggagga atcctttacg
     8821 tcggcaatat ggccgacggc cgtgttgaga atgccatttt caggaacgcg gaaatcggga
     8881 ttcaagttcg tcgcggtatc attgcagacc ggcctgcaga ggctgattct gcgaacccca
     8941 tccctgagta catactcagg cgtccgaccg gccctgttgt catccgcaac tgcacgattg
     9001 acagcgccag ttttaacggg ctcgtgtttg tcggtgtcga cagcagcgta gttgccgaga
     9061 acaatttgat ccagaattgc tcaagcggca tttcgtgcga agagggcgcg agacccattc
     9121 ttcgcctgaa tcgcatactc aacaacacga gcaccggaat catctgctcg tcggagtcga
     9181 gcccgctgat taccggcaat accgttgttg gaagctctac cgcagggatc atctgtgcga
     9241 acaactcgaa tcctacaata gacgggtgca taattgcgaa aaacggtatc ggcgtttcgg
     9301 tgacgaaatc gaacccgatg atcacgcgct gtacgatcgc aaagaacgaa ttcagcggga
     9361 tcatcgccta cgaaggagcg gcaccgattg tctcatcgtg caacatccgc gggaacgggc
     9421 tctccggcat agacaaccga tcctcgggca atattacggc ggagagggtc tggtggggat
     9481 tcatcaccac agatcagcga aaagcacccg tgctggatgg tggaaggcgc agggagaggg
     9541 gggaaccggt tccggaaggg cagaccggct cagtgcgcat cgccaattcg ctttcctcac
     9601 cctcgcttga cgcgccgggc accccaaaac aggcgcaaga gctgattctg gcacgtgata
     9661 acgcaattac aaatcgattc cctgagcgag gtgaggtatc caatggcgac accgtctgga
     9721 tccaggttcg tgccattgac gaaagccctt acctcgaaga tgaagcaatc atcgtgttcc
     9781 gcagctcttc gggtggcgca gcaggaacac aacgcgttct gaccgaatcg agtgcttcca
     9841 gcggcatata caagggttac gtgatcgctt ccctcgcaga aggcgaccca aacattgtgg
     9901 tacaggtgcg caacggtgac gtgataactg tggagacagg cacaacccct ccgctcactg
     9961 cgcgggtagt gttcgtgagc aaaccaccgg caatacagaa cctcagggta gatggccaac
    10021 gacgtggagc ccgattgaca aatccgagac ccgtgttctc gtggcgctac tgggacaacg
    10081 aaaacgaccc tcaacgcggc acggagctgg aactctcaac agattcgaca tttctcactc
    10141 aacactggaa gcacgcggta agtggtcccc tcagttcata cacgttcgat gggccgaccc
    10201 tgacgagagg gcagacgtat ttcctccgtg tccgggcgag cgacgggtac aatttcggcc
    10261 gttggtcggt gacgtcattc cacatgaaca ccccgcctcc gacacccatc ctcgcctcgc
    10321 ctgaggacgg cgcaattctg accgacccgc ggcataaacc ggttattgcc gttcgcaacg
    10381 tcaaggaccc agacggcgat gcggtcagct acattttcca ggctgcgtac ggagaagatt
    10441 tcagttcgcc aaggttgcga gcgggtggcc cggaggcaag ccaacccgtg ccggaggacc
    10501 cggcttccga tatcaccgtg tggcgtgcga tgccgacgtt gtacgaaaac acccgcatct
    10561 ggtggcgtgc gaaggcgacg gatgggacgg aggaaagtga gtggtccgct ccacggtcgt
    10621 tctccctgaa cacgacgaat gacccaccca tgccgttcgt catgctgcaa ccggaacgtg
    10681 acagcaccac caattccctt acgccgcgat tggaatggtc acactcgtac gatccggatc
    10741 cgggagagac catttcgttt gtagtggcat ttggaagaaa caaagacctc agagatgcgg
    10801 tgatccggca cgttgaaccg gtcaacgagc cagttcagtc ctaccagatt accccgtcgg
    10861 aaatgttgct ggacaacacg tggtattact ggaccgttcg gctggagcag gacgggcggg
    10921 tcctttttcc cgccaatact gctgcacgcg acacaacgtc cgtgtggagg tttttcgtcg
    10981 acaccggcaa cgacccgccg gttgtggcgg gaatcccgcc tgtcgtgatg acagaagaca
    11041 cgccctacaa gatgaacctc gggcggtaca taagtgaccc ggatcacccc actgacgtgt
    11101 tgaaggttac cgtcacgggg acagaacacc tgaccgcggc actcggcccc ggcaatgagc
    11161 tgactctgac gccggcgcag aactggtttg gcggtcccga acgagttcgc atactcgtga
    11221 tagacccgat ccttgcggaa gccaacgggg agatctctgt gtccgtgacg ccggtgaacg
    11281 acccgcctgc ggtggcgcgg gtcccggacc aatctgtcgc ggaagacacc gacttgagag
    11341 tcgatctgaa accgttcata tctgacgtag acaacacagc atcagagatg aggtggaccg
    11401 caaccgcaga cacgcgcaag ttgacggtct cttttgctga tggcgttgcc acgatccggg
    11461 gcactcaaga ttggtttgga accacaccgg tgagagttac cgcgacggac acggggggcc
    11521 tctctgcatc gtccgagttc aacgttacgg tcaccccggt caacgacccg ccggcggtcg
    11581 tacagattcc gtcgatcaga accaaagaag atgaagcggc ggtcgttcaa ctggacaact
    11641 ttgttactga cccggacaat cagaaaagcg agttgcgatg gagcgccgtg gcggaagagc
    11701 cgctgacggt gacgatcgac ccggcgacaa ggcgggcgag catatccgcg ccgccgaact
    11761 gggccggcgc agaacacaaa gtcaccttca ccgtcgcgga cccggacggt ctgagcgaca
    11821 ggatagtagt cgctgtcggt gtggaagcag taaacgatcc gcccacgatc gctccgatcc
    11881 ctgaccagcg tttcaatgaa gatgcaaccc tgacgctgga tctcgacccg tttgtctcag
    11941 acccggacaa cacagcacgg gaaatgacct ggaccgcagt gagcggcgga aacatcaagg
    12001 cggcggtaga cgccgcgaca agacgcgtca cgttcagcgc ggcgaggaac tggtacggtg
    12061 gaccggaaac gattcgcctc acggcaaccg atcccggggg gttacgagcg acgcagtcgg
    12121 tgaacgtcag cgtgatttcc gtgcctgagg ccccggtttt tgcgcgcata cctgctgtga
    12181 atatcgacga ggacgggcaa tttgttctcg cgctggatga gtatctctca gacccggacc
    12241 atcgaaacga ccagctgacc gtgagttttg tcgctccgca gaacgtgaag gtggagttcg
    12301 acaggagaac caggcgactt acgctttctg caccgccgaa ctggaatggg ggtccggaga
    12361 cagtcgctct ggaggcggtg gatccggata atgaacgcgc aagcgcgact ttttctgtca
    12421 ccgtaaggcc tgtcaacgac ccgcccgtcc tccgcgcgat cccgccggtg gcgtttcgtg
    12481 aggacgagtc aacaacattg cgactgacgg aatttgtgac ggacccggac aacacgccgg
    12541 agcagatgca atgggttatc tcgggacaag ccagattaaa ggtccagatt gcgcaaggcg
    12601 ttgccacgtt ctccgccgac aaggactgga acggcacgga aacgctgact ctcaaggcca
    12661 ctgacccggg tgggttgttc gcccagactc agatccgcgt gaccgtgacc acggtgaatg
    12721 acgcgcccgt tgtgaggcaa ttgccgccgg tcgctttcga tgaggacggg tcgtcaacgg
    12781 tgcggttgtt cgatttcgtc agcgacgtgg acaacacaaa tgagcagctc acgtgggaag
    12841 tatcgggcaa caaccgggtc aaggttgcga ttgcgagagg gaccgcgacc ttcaccgcgg
    12901 aaccgaactg gcacgggacg gagaacctga cgataaccgt ggctgatccg gatgggttgc
    12961 gcgcgtcaag ttcgttcacg gcaacggtcc gctcggtcaa cgatcctccg gtcatagccc
    13021 gcatcccacc ggtatccttt gaagaagacg gcagaacgac ggtcgatctt gcccccttcg
    13081 gctctgatgc ggatggggat gcccttacgt ggacagccgc cagcgcgaac ccgaatctca
    13141 gagtcgccgt ttccggtagc gtgctgacgc tcagtgcgac accggattgg aacggcggac
    13201 caatcaatgt caccgttacc gcgacagacc cgtcgcgagg ccaggcgacg accaccattc
    13261 ccgttaccgt gacagcggtg aatgacccgc ccacgctgaa agcgttggta cctgttacct
    13321 tcccggaaga cggcaccgcc actctgaacc tcttgaacca ggttacggac cccgacaaca
    13381 cccctgccca gatgcgctgg gaggcttcgg gcgggacaaa cgtcaaagtc agggtggctc
    13441 aaggcgtggc gacgctgacc gcggaaccaa attggcacgg ttccgaaacg ctcacgatta
    13501 ccgcaaccga ccccggaggt ttggcggctc ggggcacgtt gtccgtcgcc gtcagctcgg
    13561 tgaacgaccc accggtgatc agccgcctgt ctgcggtctc gtttgacgaa gacggcagca
    13621 caacgctcga tctcgcaccc tttggctccg atgcggacgg agacgtcatc acatggacgg
    13681 cgacttccgc caacccgaat gtgagggtcg cggtctccgg cagcatgttg accatcagcg
    13741 ctgcaccgga ttggagcggc ggccctgtta atgtgacggt cacggcaacg gatccggccg
    13801 gcgccaaggc aaccgctacg attccggtga cggtgagagc cgtgaacgat cctccggaac
    13861 tccgtgagct tccatcggtg aatttcaacg aagacagtac cgctacgctg cggctggcgc
    13921 agtatgtctc tgacaaggac aacacgcccg cgcagatgaa ctggcaggct tccggcaaca
    13981 cgaacgtgaa ggtccggttt gcgcagaacg tcgccacatt cagcgcggaa cgcgactgga
    14041 gcggctccga gtcggttacc atcaccgtgt ccgacccgca gggcggatcc gcgtcgcggg
    14101 acgtgcgggt caccgtcagg ccagtgaacg accgtccccg ggtccagcca attccagacg
    14161 caaccgtgga ggcgggcggg cagacggaga tcgatctcgc accgttcgcc agtgacccgg
    14221 acggagaccc gctgacgtgg caggtggccg gccaaacggg acgaatcacc ccggttgtca
    14281 ccggttccac cttgcggatc acggcggctg caggcgcggc aggcgtggca acggtgaatc
    14341 tcgtggtgcg cgaccctgcc ggagcgcagg tgactgcgcg ggtacggatc acaatccaag
    14401 cggcggcacc cgtgcaggct ccatcaccga gcggcggggg tactactccg tagtggaatt
    14461 ctgattgcgc ttacaagggg acaggaccgt tgtggtggat acttttcagc attttcgtcg
    14521 caccggtcct gttccttttc ctcctcgttg cggtcggcgc tctgatgcca atgcgagtcg
    14581 gagtcagtgt tcagggtcag gcgcagagcg cgaaggaatg gggaggacgg gccacatttg
    14641 gtgtacgttt tttcgggggc ctgattggtc tgggcttccg ggctgaaggt gaggcatcca
    14701 cccgaactca tcttgctggt ggggtgcttt tctggcggtg gttttttccg ctcaaatcgc
    14761 tggattccat tttcgcgaga gcccctgagg tcagggaagt actgtctgcg cccgcatcgg
    14821 cgacacgtga acctggcgtg gccctggtgc aaccaccgcc ggaaaaaggc ccggcggcca
    14881 gtacacgagt cgaaaaggac gtggaaacgg gaggccccgc cgactggaca cgccttttcg
    14941 aggactctga ccttgtagat tttccgatgg actggcaacg taccagccgc cctgaaacga
    15001 caacagccgg ggtgctcgct gatctgcgag cggcgattgc ccgcgtctgg ccttttgttc
    15061 gggaggcacg ccgtcggttc aagggcgtgg tcacactgcg ttttttccgc ctgaccgggt
    15121 cgttcggcac gggcgatccg gcgggcacgg catggctgtt gggaacggct tacgccctcg
    15181 ccggggccaa aggttccgcc gattcggtga ggcttgccgg tgatttccag agagcgattg
    15241 cagatgggcg ttgcgaagta gagtggaaag taagcacgat ccgattatgg gcggctgcac
    15301 tcgcgcttgc gtggctcaac tggaagcacg cacgcgcatt acggacaaag aaggaggcct
    15361 gacaatcgaa gccttgatgc aaacactgct ggagcggctg cgcgacctcg tgcagacgga
    15421 aacggtcatc ggcaaaccga ttgaagcggg cggggtgatc atcattccgg tttcgcgcgt
    15481 ctcggtcggt tttggggcag gtggcaacat gaatgcgcag ggcgctgccg ccggagcgat
    15541 gaaaggcgga ggcggaggcg gcggagtacg cgtggaaccc ctcgggttcg tcgtcatcag
    15601 caacggcaaa gccgaggtgt tgccgatgaa gcccgacgag cccgccatca acaagatcgt
    15661 ggacatggtg ccgggcatct gggattcggt acgttcgatg ctcgacaagc gcgacaggaa
    15721 aaaagactag gaacctgaaa cctgtgtaaa gtgtgcctgc acccgggtgc aggcacactt
    15781 tggtctccgc cggagagtcc gcggcgtacc tcctgccgca caccgaacga tcccccgtca
    15841 atcctccagg ctgaatttca gatcaggaag tggatatcca tcggcgtccg tgatgcgaaa
    15901 cgtgtacatc catccaccct tcgcgttgat ggtagtcacg ttgatgcgtg tgacccccgc
    15961 gggtatgtgg atggggactt tcgcctcgaa ttgataccgg tcgaggtaca tcgcgccgtg
    16021 cccacgctca gggtattccg cgcggtcgaa caccacgctg tcgccaacct gcacaaccag
    16081 cccgtcgtcc cacccgaggt taagccatgc gtcgaccgcc ctgggcgagt gtacataggt
    16141 ggccagatgc acactggaat tgtcagtcat caagtaatct gtaaacgtcc gcatggaaac
    16201 gatgccacgg ttgtcctgaa ccgcccaggg gaaccacctg accgccccgt cgcgcccgaa
    16261 gaacaccaga gaaggagaga tgggcatgga gagcgcttcc ttcgccgtcc gtgcgggatt
    16321 gacaaagggg ttgctcagaa cgccgagagc ccaccacttt gccggggtgc caaatgcgtt
    16381 gcagaattcg gtagcatcga tccgtccttc gtcgaatcgc ttcgtggcat cgaacagacg
    16441 tatcatgcgc ggatcagcgc ccggagtctg gtcgtaccgg actcgttccc gcaggatatc
    16501 gtgccatatc gggatgaata cttccgctcc cggatacgtg gcggagagcc cgtaccggtc
    16561 cctgttttcg gcggacggca gcagccagat gggctggccg ctgaacgacc acatgtagcg
    16621 tttcgtagtc gaacggagga tgcgcatctg ttgccgtagt tccgccatct cctcatccca
    16681 cggtctcacc gggtaatcgt cagcgcctgt ttccaccatg tggcagggcc agacgccggg
    16741 tgccatcgtg cacgtgctcc gccagtactc gagactcgtc cctgagagga accgatccat
    16801 cttcgcgtct tcgtgccgcg ggatcgccgc ctgtgtaacc ggctcgtgca gggtgtaaga
    16861 gtattccgtg ccgaagtgaa agcctccagg ggcgttccgg ttcgccatct cttctatgag
    16921 ccctttgacg aactgccggg caagaggcct cccctccaag gaacctggga gattgaaaat
    16981 caccgcgtcg gggaattcgt caagaattgc cgacatcacg atgcgcccct gcctgtatga
    17041 agcctccatc aagtcacccg gtgtgtagcc agcatacgtg taaatcgggt ggtccagcga
    17101 gtaacgcggg taggggtatt cagtgtcaat gctgattcca cggaagccag tcgccttcgc
    17161 ggctttgccc agcgctgaga aatgttgtct caacttttcg gtgaactcgc cagaaaggag
    17221 agtctccggt gaaggccatg gttcgctgtc accaaaggaa atcgcaagca gattttcggt
    17281 gcagccaacc gatttgagcg aggtgacatt ctgtccggcc agcctcagcc agttcgggtt
    17341 ggtcgcaggc gtgttgccga cgccgatgtt cgtcagcaca cccgaatccc agctgccaca
    17401 ctggaaaaag aacccgtcgt agagcccctt gacgttggcc gcatgttcgt tgaggtaggg
    17461 cgtgggtgac accgagtaac agatgatcgc gggtatagcg agagcagtgg gagcgggcat
    17521 ggagccttcc tttctctttc tttccggttc cggattgtgc cgtgggagtt ctgatgtgga
    17581 aacctgcgcg tggagtccga tcgtaacaag gcaggaaact gccatgggat gattgccgaa
    17641 atcttcgagg ttttccgtta accaaagtat gaccgaattt cccgctgcgc aacgggtggc
    17701 ctagcgtcgg cacggagact ccgcccgcgt ttgccaatga atcggaggaa ctcaactgca
    17761 acatccgggg ttattcatgg aaagcggggt gggtatcccg gagtgggtac cgcatggctc
    17821 ccccaacgcg ccacaccgag cttacttttc gaacattttg cttgcaaaag tcggaaggcg
    17881 ccggtacatt tcactgtcta cacactttgc gagcgcatcg gcaagaacac gcaacggaga
    17941 ctgggaa
//
LOCUS       DAOTNG010000018        17700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3195_length_17700_cov_4.750184, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000018 DAOTNG010000000
VERSION     DAOTNG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17700
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_17700_cov_4.750184"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5179_01725"
     CDS             <1..469
                     /locus_tag="P5179_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93972.1"
                     /translation="YPEDPGIWFPTATPEGAKTMRHFSPVHGDESTHRHAADSEEITV
                     SHDHRIYMYGSYSRFRYARPFFWGFVHGMSVLFMFDDNPNIRFAQSPSGGGIRCPAWD
                     FQLVVPDYRVGGEYSLEARMVYSRLGATLDPEVEYARWVEFRKNNRSDGGEGR"
     gene            466..816
                     /locus_tag="P5179_01730"
     CDS             466..816
                     /locus_tag="P5179_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93973.1"
                     /translation="MKDQSDGVQSWPERGERNGLTRSPIAGLDGLLCPVCGEIADSLP
                     IKVQRVIQGTEECLFVCSPDCRQMALESPFLQINMFSRDMRVWERSVQSELDIESSPV
                     AEAPETEHNGREHS"
     gene            813..1733
                     /locus_tag="P5179_01735"
     CDS             813..1733
                     /locus_tag="P5179_01735"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRS93974.1"
                     /translation="MTKAGKAMTDVELVLDAKADLGEGAIWHARRGLLYWVDINAGFL
                     HVFEPKSGKDDAYRIGEDVGTVVPRVASSERPYESVMLALRRGFAVFRFDVAKAYVVA
                     DPERDIPANRFNDGKCDPVGRFWAGTMPEGPGGVPDGGRLYCLYPDLHVEVKETGIRC
                     SNGVAWSLDHRTMYYIDTPSMEIWAYNYDLETGAIWDRRVVVRVPKNEGYPDGMTIDR
                     EGMLWVAHWDGWQVVRWNPKTGRKICSVKVPAARVTSCAFGGKNLDELYITTARTGIK
                     AEALRKQPHAGGVFRVLPGVSGLDAFEFAG"
     gene            1985..2272
                     /gene="gatC"
                     /locus_tag="P5179_01740"
     CDS             1985..2272
                     /gene="gatC"
                     /locus_tag="P5179_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRS93975.1"
                     /translation="MPVSREDVLHVAALARLEVAPEQVESFQRELGAILEHFQKLNEL
                     DTSNVEPTSHALALVNVFREDIPQPSLTQEEALANAPQKAFGHFRVPKVID"
     gene            2320..3075
                     /locus_tag="P5179_01745"
     CDS             2320..3075
                     /locus_tag="P5179_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRS93976.1"
                     /translation="MGVVPARFGSERWPGKVLENVSGKPLLRWAWETLSGDPEVSEVL
                     IATDDSRVETWARAHGMRVVKTSAESRNGTERTAEIARMIRADYYVNVQADQVALESR
                     VVTAMLRAAKAHPEWEIATAGTRWYRTGCLENRDRVFLAARGCTCGRHSIAEHFFRGG
                     EKHTNTDRPVLRHLGLYGYTPSFLREYAANEPSQDEVILHLEQCRALQMGLEIGVVRV
                     RTSARSVDSAMDRDAVEGSWSNKTSKGDPREDA"
     gene            3062..4894
                     /locus_tag="P5179_01750"
     CDS             3062..4894
                     /locus_tag="P5179_01750"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRS93977.1"
                     /translation="MRTREQTFPKYIVVAGGVISGVGKGLATSSIGRILQEFGYSCTA
                     VKIDPYLNYDAGTMRPTEHGEVWVTDDGGEIDQDLGNYERFLGKELSRRNNLTTGQIY
                     KAVIEKERQGEYLGQTVQPIPHITNEIKRRVTEASAGHDIALVEVGGTIGDYENTPFF
                     FALKSLEREIGKQNIVYVLVTYLPVPTHIQEMKTKPTQHSIKLLSEQGIFPDFILCRS
                     KSPVDEVRKRKIETYANIDMDHIISAPDVESVYSIPLNFEKENIGRKILAMFDLPERR
                     EPDWANYSTLVKRILNPRETVRIAMVGKYVTSGDFSLTDAYVSVNQALEHAGAANDTK
                     VQIDWVSGADFNDSNVSDELRAYDGILVPGAFGSSGAEGMIAAIRFARESGIPYLGLC
                     YGLQMAVVEYARNVCGLARANSREIDPNTPHPVIDIMDLQRAVIEPTVWQAWRAISQQ
                     IDIVADNWLNSRQHQIGGSMRLGAYAARLIEGSLVFRLYQESGRLESDRSRMDRLRMQ
                     PGQAFRLGREKPGAPVVLERHRHRYEVSPAYVERLERGGLVFSGYHEQADGNRLMEFL
                     ELQEHPYFVATQAHPEFRSRLEEPAPLFVGFVRAALERKHSAGK"
     gene            4891..5556
                     /gene="lptC"
                     /locus_tag="P5179_01755"
     CDS             4891..5556
                     /gene="lptC"
                     /locus_tag="P5179_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRS93978.1"
                     /translation="MKSSPVLIVVLLAQAVVMAACDDIGSSRRNAPEAALSGQRLERS
                     RIVWSDRGRKSAVVSAGELIRFDNRNEIALRDSVVVEVYDETGALVAIARGEEGTVDE
                     RSETARVTTGISVRFSGNDGKETSTLTARQAEADNRAQTVSASGNVRVRSDGGVTVYT
                     EKVIWDGRTRRFRAPGFVRLVNGTDVEEGENLDANSDLTHWTMDRVRGRSTRVREELE
                     SGS"
     gene            5559..6827
                     /locus_tag="P5179_01760"
     CDS             5559..6827
                     /locus_tag="P5179_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93979.1"
                     /translation="MAARTTTLATLFVGLVVCGTSGAQQGQRAVEFSADRTEMIRRGD
                     RFLKRYTGAVVIRFGPGEWLTADEATYDSDGRNINIAGSVVMQDSGRILRADRVQYLF
                     RDNPRAGGHPLVLLLGRVALVDNARRLNADRAEYRSHVDSLAAFGDVVAKDSSRTVTA
                     DTLLLKADGNRFVAMGRVVLEDTVENVTVRAGYYSFDGADSVGVIAREPTLVRGAGDT
                     AVTVRADSMILNVTGKKGSAWGTVRIEKGQMTAECDSLALDDNRGTIALFGRPHAVQK
                     RQEDSTSTVDHLWGDRIVLELEQSKITRILVDGSARTSSTHSDSRGGLGPERWATGSQ
                     MVFHVEHDEVREVIVWGQARSRYEPDFEDARESGTNEAAGDTIRVAFRDGKPVRVHLA
                     GGVHGLYVQNVARRTDATTRAASPDERRGG"
     gene            6820..7563
                     /gene="lptB"
                     /locus_tag="P5179_01765"
     CDS             6820..7563
                     /gene="lptB"
                     /locus_tag="P5179_01765"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRS93980.1"
                     /translation="MDDSEIRSSLRAEGLSKRYGGRLVVNHVSLVVKRGETVGLLGPN
                     GAGKTTTFYMLLGMVVPEEGRVYLEEQEITKLPMYRRARLGIGYLSQERSVFRKMTVE
                     QNILAILERLPLTREERMQRLEEILDELDIARVRKTRGEQLSGGECRRTEIARALVIE
                     PKFLLLDEPFAGIDPIAVEDIQSIVARLKAKGLGILITDHNVHGTLAITDHAYVMAEG
                     GILCAGTPAELADNELARKFYLGERFRLN"
     gene            7604..9001
                     /gene="rpoN"
                     /locus_tag="P5179_01770"
     CDS             7604..9001
                     /gene="rpoN"
                     /locus_tag="P5179_01770"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRS93981.1"
                     /translation="MRQELRLQQRLAPQLIQSLHLLQLPALDLEQVVREELETNPLLE
                     EVPAGELEHGKEEPAGETGEEADATSDIDLDATEWSDYLSDSYELSQTFREEPDHSAE
                     TYEPVSVYQETLADDLREQLRFVLSDETELKIGEYIIGNLDSNGFLATTVEEIAATLG
                     VPGTQVQKVLKAVQTLDPPGVGARDLRECLLIQLRERGYEEDPAYEIVDKCFDELMHK
                     RTKEIMRITRLSKEDVAEALEVISTLNPKPASVAYGSSERPVTPDLIVERVDGEYVVS
                     LNDRSIPALRISQNYRELLAGGGTEETKKYIVDKLNSAKWLIKSIEQRRTTMLKVMNA
                     IVKHQRAFFDYGLDHLKPMVLQEIADEIKMHVSTVSRVSNGKYVQTPHGVFELKFFFD
                     GKLGVSDGEDMASKVVKEKVKQMIDAEDKQNPLSDQAIADRLTRESGIEIARRTVAKY
                     RDQLRIPSARMRKEV"
     gene            9003..11405
                     /gene="sppA"
                     /locus_tag="P5179_01775"
     CDS             9003..11405
                     /gene="sppA"
                     /locus_tag="P5179_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRS93982.1"
                     /translation="MSFDRFWSPVVLSMLFSGSLFASTPFVTSTDRFRSQLGIIENPA
                     LSDEASAVWVNPAGLGVRTSATMFSSVAMRNNPWYVNLLFASNGSGFGWQRTDAGGGQ
                     RIDRWRFGGSGGSSPYGLSFGAAVELSDPDGLNENLFWSGDLGVLARPVTWMSAGLVV
                     RQLGARRGYPWSVESGLALRPFGPNLSIFGGLAYCEDDPLSDPSHWHAGALANVGPGL
                     EAYGAINQNRTILVGVQMILGRGSIGGAGSRVSGGSLGSGWVIARSHADYRSNRLAMK
                     GRIAEIRLKGEIRDQTPGFSLFGNRGTTLSELVMQINRAAQARDVGGLYLRFDNLAIG
                     QGMAEELRDALIKFKANSGKPIVAYLPEASFREYFIASVADSIFLEPVGDLRLTGYGV
                     GQLYFRRALDKLGVEADFTRIGRYKSAAETFTDSTMSDATREQYEELLDDWYTRTVDG
                     IAVSRRLSADSVKALVNNAPYMAAEAVRVGLIDSAGHSDRAYESVETMVRSREGRVSG
                     KINLAHRRLYDETWGPRPKLAVIFASGQIVNGTSGEDFFSGTQMMGAETIAKALKQAR
                     EDDAIKAVVFRIDSPGGLALGSDIIWREVQLLWETDKPVVVSVGDLAASGGYYIACRA
                     DTIISNPGAIVGSIGVFDGKMVVERLARRLGIDVELLARGDNAAINSSLASRTPEQRR
                     RVAENVREVYDVFVNRVATGRGMEAASVDSIGQGRIYTAANAVSIGLVDKLGGLDEAI
                     RTAARMARLRGEVELVTMPRRTNVLETVIQSSLQDAMGVSTRQSLAGEVYFFDPIAAS
                     LR"
     gene            11483..14599
                     /locus_tag="P5179_01780"
     CDS             11483..14599
                     /locus_tag="P5179_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS93983.1"
                     /translation="MRDVVTGTTGFAAPAAKSLLAGAIVLCLMCSHAHAQVGEFGRNK
                     PTSATGNWHYVQTPHFDIYFYEGMDAIARIATTIAEEAYSQISASFDYRLRNRVPIVL
                     YRSQGQFSQTHITPSLIGEGVGGFTEFLKNRVAVPFTGSYPQFRHVLHHELVHAIVHD
                     MLYGRDILTGVQQSDIPLWFIEGLAEYESSPWTSESDMWMRDAVINDYLQLDGYFAYK
                     GGQSIMYYIAEVYGPDKVAQILNRLRSFSRFGPTVQAVLGVELEDFGKRWEKDLKKVY
                     WPEIVDRDEPEDIGQSLTDHTKDNSYFNRNPSFSPFGDKIAYLTGRDYYTSIYVMSAI
                     DGKVLWKVVTGETSGSFEEMHWQRAGIAWSPDAQRIAFAAKATSGDRLYIVDARNGRR
                     LDAFDFGFDGMFSPDWSPDGTTIVFVGVRDGKSDLYTLRLSDRSIDRLTDDYFDETDP
                     KWSPDGRRILFASDRAPDVSDVSAQLLYQTSDVYVMDVETRAMRRLTADPADDLSPAW
                     GPEGKHIAFVSDRNGIFNLYVAALDSGEGSIDSATRPLTNILTGAFAPSWNRRGDKIA
                     FSGFNKGGWDVFVIRNPLDSLREEPLRPAYFLREGKISYSSRHRHLATSTQSATSPDS
                     TAGFSIGPRTSPFSSPALDILAFTRADSARGGFVPKPYRSQLQVDNVSGQANYSTLGG
                     VGGQLYVTASDLMGFHQLQFNTSLYRDLRDSDYGVFYQYLKRRTDYAAYFQQQRTWYP
                     FQSVDYYGGIVESGLVESREISTGVYASRPTSQFRRFEAGIDYVNAKQSVLYDYLSYG
                     TGIAASQEATIEALLTRVAFVHDNALWNMFGPVVGQRWRMTVFAAPPRANTAPFGVVT
                     ADVRRYWRVSKNTSLALRGAGGRSFATHAQNAPLFYVGGLPYWINYDYENVQSSQLVN
                     DAFSQFVFPLRGTNYYELRGRSFAILNAEYRFPLIYFLATGPLNFVLSNVGGVVFLDV
                     GRTWEDPTQPSDPNIARHAQGPLGGYGYGMRINLGVLVLRMDVAWRTDLRETIGSARY
                     YWSFGYDF"
     gene            14687..15520
                     /locus_tag="P5179_01785"
     CDS             14687..15520
                     /locus_tag="P5179_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRS93984.1"
                     /translation="MEDSARTPAHFLSPDLPSVTSRHAEVLRRIRVVLVEPKYPGNIG
                     SVARAMTVCGLTRMHLVNPVPRWRESDEAWTFACGSRSVLEQATEAKNLEEALRGATT
                     VVGTTHRMGRGRGPLLPPRKLAEHLPGMLSKGEVALVFGREDKGLSNDELALCQMGVH
                     IPTASTYPSLNLSHAVMVVAYECFYAVVKAGDENVQVSGVSYPEIVALSERIAHLTAR
                     TGFRHRRGEVGFERSLRRAIERSSLSARDIALAHLVCRQVEQYIDRLEEQIRNERETS
                     N"
     gene            complement(15603..15941)
                     /locus_tag="P5179_01790"
     CDS             complement(15603..15941)
                     /locus_tag="P5179_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRS93985.1"
                     /translation="MKKVEAFIRPHLVEDVRTALTDAGVKGLSVLEARGFGHQRGHTE
                     FYRGAEYQVLFVNKVKIEAVVLDDQIDAVVNALLRVARTGKVGDGKIFVSTVDDAIRI
                     RTGESGNAAL"
     gene            complement(15981..17264)
                     /locus_tag="P5179_01795"
     CDS             complement(15981..17264)
                     /locus_tag="P5179_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRS93986.1"
                     /translation="MNRRLMAFLTLLLGIVPPAFAEGSGSIDSGDTAWMLTSTALVLF
                     MTPGLALFYGGMVRSKNVLGTIMHSVFAMGLVGIHWVVIGYSLSFSKGNSFIGGFSHA
                     FLNGVGVEPMGSIPHLAFVAFQGMFAIITPALITGAFAERMKFRAYVAFVLLWTTVVY
                     APVCHWVWASDGWLFKLGALDFAGGTVVHMSSGFSALVAAIYIGRRVGYPKDQILPNS
                     LIWTVLGAGMLWFGWFGFNGGSALASGALATLAFVTTQIAAAAAAMAWAFAERIRTGK
                     VTVLGVASGMVAGLVAVTPASGFVGPMAALAIGLAAGVICYWAICLKPRFGYDDSLDV
                     FGVHGVGGTLGALLTGVFASTYWNANGKDGLLLGNVDPLLVQALSVVATAAYAAAATW
                     IILKLIDIAGGVRVSQAEEREGLDLTQHGETAYHL"
     gene            complement(17469..>17700)
                     /locus_tag="P5179_01800"
     CDS             complement(17469..>17700)
                     /locus_tag="P5179_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS93987.1"
                     /translation="LVPLYGAMSCAYYTKRTFVNDPDWMFLWEGYTGTTEFRREDRLE
                     LTLYTLDALLHAPFVPDYWYSVFASLILDGIA"
BASE COUNT         3885 a         4743 c         5520 g         3552 t
ORIGIN      
        1 gtacccggag gatccaggga tctggtttcc tactgcaacc ccggaaggcg cgaaaaccat
       61 gcgacatttc tcccccgttc acggtgacga aagcacgcac cggcatgccg cggacagcga
      121 agagattacg gtgtcacacg atcatcggat ttacatgtac ggcagttatt cgcgattccg
      181 ctacgcaaga ccttttttct ggggatttgt gcacggaatg agcgtgctgt tcatgttcga
      241 cgacaatcca aatatcaggt tcgcgcaatc gccatcggga ggtggaatcc gctgtcccgc
      301 gtgggatttc cagcttgtgg tgcccgatta ccgcgtcgga ggggagtatt cacttgaggc
      361 gagaatggtg tactcccgtc tcggcgcaac gctggatcct gaagtcgaat acgcacgctg
      421 ggtggaattc cggaagaata acagaagtga tgggggcgaa ggtcgatgaa agatcaaagc
      481 gatggcgtgc agagctggcc ggagcgcggt gaaagaaacg ggttgacacg gtcaccgatt
      541 gcgggactcg atggtcttct gtgcccggtg tgcggagaaa ttgccgattc gctcccaatc
      601 aaggttcaaa gagtgattca gggtaccgag gaatgcctgt tcgtgtgctc gccggattgc
      661 aggcagatgg ctctggaatc accgtttctg cagatcaaca tgttctcccg tgatatgcgg
      721 gtctgggaga gaagtgtgca atcggaactt gacatcgaat cttccccggt agccgaggct
      781 ccggaaacgg agcacaatgg gagagaacac tcgtgacgaa agctgggaaa gcgatgaccg
      841 acgtggaact ggttctggac gcgaaagcgg atcttggtga aggtgcgatc tggcatgcgc
      901 gtcgcgggtt gttgtactgg gtagacataa acgccggttt tctgcacgtg tttgagccga
      961 aaagcgggaa agacgacgcg tatcgcatcg gcgaagacgt ggggactgtc gtgccgaggg
     1021 ttgcgagctc tgaacggccc tatgagagcg taatgcttgc gttgcggcga gggtttgccg
     1081 tcttccgttt cgatgttgca aaggcatatg tggttgcgga tccggaaagg gatatacctg
     1141 caaacaggtt caacgacggc aaatgtgatc cggtcggtcg cttctgggcg ggaaccatgc
     1201 cggaaggccc cggtggcgtt ccagacggcg gacgccttta ctgcttgtat cccgaccttc
     1261 acgtggaagt caaagaaacc ggtattcgtt gctccaacgg cgttgcgtgg agcctcgatc
     1321 acaggacaat gtattacatc gacacgccct caatggagat ctgggcgtac aactacgacc
     1381 tcgaaaccgg ggcgatatgg gaccgccgtg tggtggtgcg cgtgccgaaa aacgagggat
     1441 accccgacgg gatgacgatt gaccgtgagg ggatgctctg ggtggctcac tgggatggat
     1501 ggcaggttgt ccgctggaac ccgaagacag ggcgaaagat ctgttcggtg aaggtccctg
     1561 cagcccgtgt gacttcctgc gcgttcgggg ggaagaacct cgacgagttg tacatcacca
     1621 cggcacggac aggcatcaaa gccgaagcgc tgcgcaaaca acctcatgca ggcggagtat
     1681 tccgggtcct gccgggtgtc agtggactcg acgcattcga attcgcaggc tgagtcccga
     1741 cgacggtgcg agcgttgtga gaggagatcc ggggggcggg cactgtgacc cgcctctttc
     1801 cgtttttggg tggcctcacc gtgcgcaacc gacgacagat ttggcgtcac gacactggtt
     1861 gtgccgcgtt cacgccgctt cgttatcttt aaaagggtga gcaaaaaaca ttccttcatg
     1921 cacaaagttg cgcataacgg gcgctccaaa cggtgcagcg tttgttttgg aagggggttc
     1981 gcagatgccg gtaagccgtg aggacgtgct gcacgtcgcc gcgctggcaa ggctggaagt
     2041 tgctccagaa caggtggaga gttttcaacg agagctcggc gcgattctgg agcatttcca
     2101 gaagctgaac gaactggaca cctccaacgt ggagccgact tcccacgcac tggctctcgt
     2161 gaacgtgttc cgcgaagaca ttccacagcc ctcgttaacg caggaggaag cgctcgcaaa
     2221 cgctccgcag aaggcgttcg gacatttccg agtgcccaag gtcattgact gacgccattt
     2281 tcgagaaaac cgagacgggt gttgccggtg tggaaggtag tgggggttgt tcctgcacga
     2341 ttcggttcgg agagatggcc cgggaaggtg cttgaaaacg ttagcggtaa gcctctcctt
     2401 cgctgggcat gggaaaccct ctccggagat cccgaagtct cggaagtgct gatagcgacg
     2461 gacgattccc gggtagagac atgggcgcgt gcacacggaa tgcgtgtggt gaagacgtcg
     2521 gcggaatccc ggaatggaac cgagcgaacc gccgagatcg cccgaatgat ccgtgccgat
     2581 tattacgtga acgtccaggc cgaccaggtg gcacttgagt cccgggtggt gaccgcaatg
     2641 cttcgggctg cgaaagccca ccctgaatgg gagatcgcga cggcgggcac gcgctggtac
     2701 cgcactggat gtctcgaaaa ccgagaccgg gtgtttctcg cagcaagggg ttgcacctgc
     2761 ggcaggcaca gtattgcgga gcactttttc cgaggcggcg agaagcacac gaacaccgac
     2821 agaccagttc tgcgacacct tgggttgtac ggctataccc cgtccttctt gcgggaatac
     2881 gccgcgaatg agccgtcgca ggatgaggtc atcttgcatt tggaacaatg ccgcgcgttg
     2941 cagatgggct tggagatagg agtagttcgc gttcggactt ctgcgcggag cgttgacagc
     3001 gccatggaca gggacgccgt cgaaggatcg tggagcaaca agacaagcaa aggagaccca
     3061 cgtgaggacg cgtgaacaaa cgttcccgaa atacatcgtt gttgcgggag gagtcatcag
     3121 cggcgtcgga aaaggccttg caacctcttc catcggcagg atccttcagg agttcgggta
     3181 cagctgtact gcggtcaaaa tcgacccgta cctgaactat gacgcaggca cgatgcggcc
     3241 gacggaacac ggcgaggtgt gggtgacgga tgacggcgga gagatcgacc aggacctcgg
     3301 caactatgaa cgatttctcg gaaaagaact atcgcggcgg aataacctta cgacgggtca
     3361 gatttacaaa gccgtgatcg aaaaagaacg gcagggcgaa tacctcgggc aaacagtgca
     3421 acccatccca catatcacca atgagattaa aaggagggtt accgaggctt ccgctggtca
     3481 cgacatcgcg ttggtcgagg tcggtggaac gatcggtgac tatgagaata cgccgttttt
     3541 ctttgcactt aagagtcttg agcgcgaaat cggcaagcag aacatcgtgt acgtgctggt
     3601 tacgtacctg ccggtgccaa cacacatcca ggagatgaaa accaaaccga cccagcactc
     3661 catcaaactg ctcagcgaac aggggatatt cccggatttt atcctgtgcc gatcgaaatc
     3721 gccggtggac gaggttcgaa aacggaaaat cgagacctat gccaacatag acatggacca
     3781 cattatcagc gcgccggatg tggagtcagt gtacagcatc ccgctgaatt tcgagaagga
     3841 gaacatcggc cggaaaatcc ttgccatgtt cgacctgccc gagcgaagag agcctgactg
     3901 ggcgaattac agcacgctcg tgaagcgaat cctcaatccc agggaaaccg ttcggattgc
     3961 catggtcgga aaatacgtca caagcggaga cttctcgtta accgacgcgt acgtgagcgt
     4021 gaatcaggcc ctggaacatg caggtgccgc aaatgacaca aaagtgcaga ttgactgggt
     4081 aagcggggcg gatttcaacg atagcaacgt ttcagatgag cttcgtgcgt acgatgggat
     4141 actggttccc ggtgcgttcg gctcgtccgg agcggaggga atgatcgcgg cgatccgttt
     4201 tgcgcgcgag tcgggtattc cgtatctcgg actttgttac ggccttcaaa tggcggtcgt
     4261 ggaatacgct cgcaatgtgt gtggactcgc ccgggcaaat tccagagaaa tcgatccgaa
     4321 cactccgcac ccggtgatcg acatcatgga tctgcaacgg gcggtgatcg aaccgactgt
     4381 gtggcaagcc tggcgggcca tcagtcagca gatagacatc gttgcggaca actggctgaa
     4441 cagcaggcaa catcagatcg gtggctcaat gcgtcttggc gcctacgccg cacgcttgat
     4501 agaaggatct ctcgtgtttc ggctgtacca ggaatccggc cgcctcgaga gcgatagatc
     4561 caggatggat cgcttgcgca tgcaaccggg acaggcattc cgtctcggga gggaaaagcc
     4621 tggagctcct gtcgtcttgg aacgccacag gcaccggtac gaggtatcac ctgcctacgt
     4681 ggaacggttg gagagagggg ggctggtttt cagcgggtac cacgaacagg cggacggcaa
     4741 ccgattgatg gaattcctcg aactgcagga acacccgtat ttcgttgcga cccaggcgca
     4801 tcctgagttt cgttcgcgtc tggaagaacc ggctccactc tttgtcggtt ttgtcagggc
     4861 tgcccttgag aggaaacatt ccgcaggaaa atgaaaagct cgccggtcct tatcgtggtg
     4921 ctgctggcgc aagcggtcgt gatggcagcc tgtgacgaca tcggctcgtc ccgcagaaac
     4981 gcgcctgagg cggctctttc agggcaaagg ctggaacgat ctcgtatcgt gtggtcggac
     5041 cgaggaagga aaagcgcggt agtttccgcg ggagaactta tcagattcga caatcgcaat
     5101 gagattgctt tgcgcgattc cgtggtcgtg gaagtgtacg atgaaacggg tgccctcgtt
     5161 gccattgcgc ggggcgaaga gggcacggtg gacgaacgca gcgaaacagc ccgggtgaca
     5221 acaggcattt ctgttcggtt ctctggaaac gacgggaagg aaacgtccac cctcaccgca
     5281 cggcaggccg aggcagataa ccgcgcgcaa actgtctcag cgtcgggcaa cgttcgggtt
     5341 cgcagtgatg ggggcgtaac ggtgtatacc gaaaaagtga tctgggacgg tcggacgcgc
     5401 aggttccgtg cgcctggttt cgtccgcctg gtcaatggaa cagacgtcga ggagggggaa
     5461 aacctcgatg cgaacagcga tttgacccat tggacaatgg acagagtgcg cggccgaagc
     5521 actcgcgtgc gggaagaact ggagagcggc tcctgaacat ggcggcgcgg acaacgactc
     5581 tggcgaccct gtttgtgggt ctggttgtct gcggaacctc cggcgcacaa cagggtcagc
     5641 gcgccgttga attcagcgct gaccggaccg agatgatacg tcggggagac cgcttcctga
     5701 agcgttacac cggtgcggtg gtcatccgct tcggacccgg ggaatggctg acggctgacg
     5761 aggctacgta cgacagcgac ggcaggaaca taaatattgc tggatcggtg gtgatgcagg
     5821 actccgggcg aattctgcgc gccgaccggg ttcagtacct ttttcgggac aatccacgag
     5881 cgggtggcca cccgttggta ttgttgcttg ggagggtcgc gctggtcgat aatgcccgtc
     5941 ggctaaacgc tgaccgcgcg gagtaccgct ctcacgtgga tagtctcgcc gccttcggcg
     6001 atgtggtggc gaaagacagc agcaggactg ttaccgcaga tacccttctt ttgaaggcag
     6061 atggaaaccg gtttgttgca atggggagag tggtgctgga agataccgtc gaaaatgtaa
     6121 cggtccgcgc ggggtactac tccttcgatg gggcggacag cgtcggtgtg attgcgcggg
     6181 aaccgacgct tgttcgcggc gctggcgata ctgcggtgac ggtgcgcgct gacagcatga
     6241 tactgaacgt tacgggaaaa aaaggaagcg cgtggggaac cgtgcggatc gaaaagggcc
     6301 agatgaccgc cgagtgcgat tcgctggcgc tggatgacaa tcggggtacg attgcgcttt
     6361 tcgggcgccc gcacgccgta cagaagcggc aggaggactc caccagtacg gttgaccatc
     6421 tgtggggaga ccgcatagtg cttgagttgg agcagtcaaa gattacgcga atacttgtgg
     6481 acggttcggc gcgtacaagc agcacccata gtgactcccg tggagggttg gggcccgaac
     6541 gctgggcgac cggttcccag atggtgttcc acgtggaaca cgacgaggtt cgtgaggtca
     6601 tcgtctgggg acaggcgagg agccgttacg aaccggattt tgaggacgcg cgcgagagcg
     6661 gcacgaacga ggcagcaggc gacacgatac gcgtcgcctt ccgcgatggg aagcccgttc
     6721 gtgtgcactt ggcgggagga gtgcacggtt tgtacgtcca gaacgtcgca cgtcgaactg
     6781 acgcaacaac acgcgctgct tccccggacg aacgacgtgg tggatgattc ggagattcga
     6841 agttcccttc gagcggaagg gctttccaaa cgctacggcg gacggctcgt agtcaaccac
     6901 gtctcgcttg tggtgaaaag gggtgaaaca gttggcctgt tgggcccaaa cggtgccggg
     6961 aaaacaacga ctttctacat gcttcttggg atggtcgttc ccgaggaggg gcgtgtatac
     7021 cttgaagaac aggaaatcac aaagctcccg atgtaccggc gggcgcgttt gggaataggg
     7081 tatctcagcc aggaacgttc tgtctttcga aagatgacag tcgagcagaa catcctcgcg
     7141 atccttgaac gcctcccgct tacgcgggag gaaagaatgc agcggctgga ggaaatcctg
     7201 gacgagctgg acatcgctcg cgtgcggaaa accagaggcg agcaactgtc cggcggcgag
     7261 tgcagacgca ccgagatagc acgcgcgctg gtcatcgaac cgaagtttct tctgctggat
     7321 gaaccgtttg ccggaataga ccctattgcc gtggaggaca tccaatcgat tgtcgcgcgg
     7381 ctgaaggcca agggattggg tattttgatt accgaccaca atgtacatgg cacactggcc
     7441 atcaccgatc atgcgtacgt gatggcggaa ggcggtattc tgtgtgcggg cacacccgcc
     7501 gaactggcag acaacgagtt ggcccgcaag ttttacctgg gtgaacgttt ccgtttgaat
     7561 tgattcagtg gtatcggtcg caagggtttc gccagtgctg ggaatgcgtc aggaactcag
     7621 gcttcaacag cggcttgcgc cgcagttgat tcaatccctc cacctcctcc aattgccggc
     7681 gcttgatctg gaacaagttg tcagggagga gcttgaaacg aatcccctgc tggaggaggt
     7741 tccggctggc gagttggaac acggaaaaga agagcctgcg ggggaaacgg gtgaggaggc
     7801 ggatgcgacc tccgatatcg atctggatgc aactgaatgg tccgactacc tcagcgactc
     7861 gtatgagctg tcgcagacct ttcgcgaaga acccgatcac tctgcggaga cgtacgagcc
     7921 cgtatcggtg taccaggaga ccctggcaga cgacctgcgc gaacagctcc gcttcgttct
     7981 ttccgacgaa acagagctga aaatcggcga gtacattatc gggaacctcg acagcaacgg
     8041 ttttctcgcg acaacggttg aagagattgc ggccacgctg ggagtgccgg gaacacaggt
     8101 ccagaaggtt ttgaaggcag ttcagacgct ggacccgccg ggtgtcggtg cgcgcgatct
     8161 tcgggagtgc cttctcatcc agctgcggga acgcggttat gaagaggatc cggcctacga
     8221 aatagtggac aagtgcttcg acgaactgat gcacaagcgg acgaaagaga tcatgcgcat
     8281 cacgcgcctg agcaaagaag acgtagcgga ggctctcgaa gtaatatcca cgctgaaccc
     8341 gaagccggct tccgtcgcct acggttcttc cgaacgcccg gtaacaccag atctcattgt
     8401 cgagcgtgtg gacggggagt acgtggtttc tctgaacgac cgatccatac cggcactcag
     8461 gatcagccag aactatcgag agctccttgc gggcggtggt accgaagaaa cgaagaaata
     8521 catcgtggac aagttgaatt cggcgaaatg gctcatcaaa tccatcgaac aacgccgtac
     8581 gaccatgctc aaagtcatga acgccatcgt caaacaccag cgagcgtttt tcgactacgg
     8641 gcttgaccat ctgaagccca tggttctgca ggaaatcgcc gacgagatca agatgcacgt
     8701 ttccacggtg agccgcgtat cgaacggcaa gtacgttcag acgccacatg gtgtgtttga
     8761 actgaagttc tttttcgatg gcaagctcgg tgtttccgat ggcgaagata tggcctcgaa
     8821 agtggtcaag gaaaaagtga agcaaatgat cgacgcagag gacaagcaga acccgctcag
     8881 cgaccaggcg attgccgacc ggctgacccg cgaaagtggt atcgaaattg ccaggagaac
     8941 cgtggcgaaa taccgtgacc aactccgcat cccatccgca cgcatgcgga aggaggtttg
     9001 atttgtcgtt cgacaggttc tggtcgcccg ttgttttgtc aatgctgttc tccgggagtc
     9061 ttttcgccag cacaccgttt gtaacctcca ctgaccgttt tcgaagtcag ctcgggatta
     9121 ttgagaaccc cgctctttcc gatgaagcga gcgcggtgtg ggtgaacccc gcagggcttg
     9181 gtgtgcgcac atcagcgacg atgttttcct cggttgcaat gagaaacaac ccctggtacg
     9241 tcaacctgct ttttgccagc aatggaagcg gtttcggctg gcagcgaacg gatgccggcg
     9301 gcggccaacg aattgaccgc tggcgcttcg gaggaagtgg cggaagctcc ccgtacggcc
     9361 tttcattcgg cgctgcggtc gaactgagtg atccggacgg tttgaacgag aatcttttct
     9421 ggtcagggga ccttggtgtt ctcgctaggc cggttacgtg gatgtccgcc ggccttgtcg
     9481 tgcggcaact cggcgctcgc cgtggttatc cctggagcgt ggaatcgggc cttgctctgc
     9541 gcccgttcgg accgaacctg agcatttttg gcggtctggc gtactgtgaa gacgacccat
     9601 tgagtgatcc ttcgcactgg catgccggtg cactggcgaa cgtggggcct ggtcttgagg
     9661 cgtacggggc gatcaatcag aacaggacga ttctggtcgg cgtccagatg attctcggaa
     9721 gaggcagcat cggcggagca gggagtcgcg tgagtggcgg aagcctcggt tcaggctggg
     9781 taattgctcg ctcccatgcg gattaccggt ctaaccggct ggcgatgaaa ggcaggattg
     9841 cagagattcg gctgaaaggt gagattcgcg atcagacgcc gggattcagc ctgtttggca
     9901 accggggcac cacgctttcg gaactcgtca tgcagatcaa tcgggcggcg caggcgcgcg
     9961 atgttggtgg gctgtacctt cgttttgaca acctggcgat cgggcaggga atggcggagg
    10021 aattgcgtga tgcgctgatc aagttcaagg cgaactcagg caagccgatt gttgcttatc
    10081 tgccggaggc gagtttccgg gaatacttca ttgcctccgt tgcggacagc atcttcctgg
    10141 agccggtggg cgacctccgt ctgaccgggt acggcgtggg gcaattgtac ttccgcaggg
    10201 cgttggacaa actcggcgtc gaagctgatt tcacccggat aggccgttac aagtcggccg
    10261 ctgaaacgtt cacggacagc accatgtcgg atgccacccg ggagcagtac gaggaactcc
    10321 tcgacgattg gtacacgaga acggtagatg gaatcgcggt gtctcggcgc ctcagcgctg
    10381 acagcgtgaa ggctctggtc aataacgctc cctacatggc tgcagaggca gttcgtgtgg
    10441 gcctgattga ttccgcaggt cacagtgatc gagcctatga atccgtggaa accatggtaa
    10501 ggtcgagaga ggggagagta tcgggcaaaa taaaccttgc gcaccgtcgt ttatatgatg
    10561 agacgtgggg cccgcgtccg aaactggcgg ttatcttcgc gtccgggcag attgtgaatg
    10621 gaacgagcgg ggaggatttc ttttcgggaa cccagatgat gggtgccgag acaattgcga
    10681 aggcgctcaa acaggctcgg gaggacgatg ccattaaggc ggtcgtgttc cggatcgatt
    10741 cccccggggg actggctttg ggcagcgaca tcatatggcg ggaggtacag cttttgtggg
    10801 aaaccgacaa gcctgtcgtc gtaagtgtgg gcgatctggc tgcttcgggc gggtattaca
    10861 tcgcgtgtcg cgcagacacg atcatttcga atccgggcgc catcgttggc agcatagggg
    10921 tatttgatgg caaaatggtt gttgagagac tggcgcgccg actcggaatc gatgtggaac
    10981 ttctcgcgag gggagacaac gcggcgatca attcttcact cgcatcgcga acaccggagc
    11041 agcgaaggcg agtggccgaa aacgttcgtg aagtctacga cgtgtttgtg aacagggtcg
    11101 ccacgggccg cgggatggaa gccgcctcgg tggattccat cggtcagggc cggatttaca
    11161 cggcagcgaa tgccgtcagc ataggcctcg tcgacaaact gggtggtctg gatgaggcta
    11221 tccggacggc cgcccggatg gcccgcctgc gcggcgaagt cgagctcgta acaatgccga
    11281 ggcgcacaaa tgtgctggaa acggtgatcc agagctcgtt acaggatgcc atgggtgtgt
    11341 cgacccggca gtcactggcc ggagaggttt acttcttcga cccgattgcc gcgtcgttgc
    11401 gatgacacgg gtggtttttg gatccggggt tttgaatatt gcgggccgtt tctggggtta
    11461 cacggttgta cggatgtgta gaatgaggga tgttgtgacg ggtaccaccg ggtttgcagc
    11521 gccagcagcg aaatcgctgc tggctggcgc gatcgttttg tgcctgatgt gctcccacgc
    11581 gcatgctcag gtgggtgaat tcggccgcaa caagcccaca tccgccacgg gaaactggca
    11641 ctatgtccag acgccgcatt tcgacatcta cttttacgag ggcatggacg cgatcgcgag
    11701 aatcgccacg acgatagccg aagaggcgta ttcccagatc agtgcgagct tcgactaccg
    11761 cctgcggaac cgcgtgccga tcgtgttgta ccggtcccag gggcagtttt cgcagacgca
    11821 catcacgccc tcactgatcg gtgagggtgt tggcgggttc acggaatttc tcaagaaccg
    11881 cgttgccgtg ccattcaccg gatcctaccc gcagtttcgt catgtcctcc accacgagct
    11941 cgtacacgcg atagtgcacg acatgcttta cgggcgagac attctgaccg gcgtacaaca
    12001 gagtgatatc cctctctggt tcatcgaagg tcttgcagag tacgagtcgt cgccgtggac
    12061 ttcagagagc gacatgtgga tgcgggatgc ggtgatcaac gattacctgc agctcgacgg
    12121 ttacttcgcc tacaaaggcg gccagtcgat catgtattac attgctgagg tgtacgggcc
    12181 ggacaaggta gcgcagatcc tgaaccggct tcgctctttt tctcgcttcg gtccgacggt
    12241 tcaagccgtt ctgggggtcg agctcgaaga tttcggaaaa cggtgggaga aagacctcaa
    12301 gaaggtgtac tggccggaaa tcgtcgatcg agacgagcca gaggatatcg gccaatcgct
    12361 gacagaccac accaaagaca actcctactt caatcggaac ccctcattct caccgttcgg
    12421 cgacaagatc gcgtatttga cgggcaggga ttactacacc agcatctacg tgatgtcggc
    12481 catcgacgga aaggtgctct ggaaagtcgt tacgggtgaa acttccgggt cctttgagga
    12541 aatgcactgg caacgggcag gaattgcgtg gtctccagat gcgcaacgga tagcgtttgc
    12601 ggcaaaggct acgtcgggcg acaggcttta catcgtcgat gcgcgcaacg gacggaggct
    12661 cgacgcgttc gatttcggat tcgatggaat gttttcgccc gactggtcac cggacggaac
    12721 tacgattgta tttgttggcg tgcgtgatgg gaaatcggac ctctacacac ttcggctttc
    12781 ggaccggtcg attgaccggc tgacggacga ctactttgac gagactgatc caaaatggtc
    12841 gcccgacggc cggcgaatcc tgtttgcttc cgatcgagcg cccgatgtga gcgatgtttc
    12901 agcccagttg ctgtaccaga catcggatgt gtacgtgatg gatgtcgaga cgcgcgcgat
    12961 gcgccgcttg acggcggacc ccgcggacga tctttcgccg gcatgggggc ccgaagggaa
    13021 acacatcgcg ttcgtgtccg accgcaacgg tattttcaac ctgtacgttg ccgcgctcga
    13081 cagcggcgaa ggaagtatcg acagtgcaac ccgtcctttg acaaacatcc tcacgggagc
    13141 gttcgctccg tcctggaatc gcagaggcga caagatcgcc ttctccggtt tcaataaggg
    13201 ggggtgggac gtttttgtca tcaggaatcc actggacagc cttcgtgagg aaccccttcg
    13261 gccggcatat ttcctgcgcg aagggaagat ttcgtacagc agccgccacc gccacctggc
    13321 gacgagcacg cagtcggcca cgtcacctga ttccaccgcc ggattctcga tagggcctcg
    13381 caccagccca ttcagttccc ccgcccttga tatccttgct ttcacccggg cagattccgc
    13441 gcgtggcggc ttcgtgccaa agccttatcg aagccaactc caggtagaca acgtaagcgg
    13501 acaggcgaat tactcaactc tcggtggagt cggcgggcag ctctacgtca cggcgagcga
    13561 tctcatgggg tttcaccagc tccagttcaa caccagtctt taccgggacc tcagggacag
    13621 cgattacggt gtgttttatc agtatctgaa aaggcgaaca gactacgccg cgtatttcca
    13681 gcaacaacgg acatggtatc ctttccagag cgtggattat tacggcggga tcgtggaatc
    13741 cgggttggta gaaagccggg agatttcaac cggcgtttat gcgtcccgcc cgacgagcca
    13801 gttccgccgg ttcgaagcgg gcatagacta tgtgaacgcg aaacagagtg tgctttacga
    13861 ctacctcagc tacggcaccg gcattgcggc gtctcaggaa gcaaccattg aggctttgct
    13921 tacaagggtt gcgttcgtgc acgacaacgc gctatggaac atgtttggtc cggtggtagg
    13981 gcagcgctgg cgtatgacgg tttttgctgc gccgccgcgc gccaacactg ctccattcgg
    14041 cgtagtgacc gcggatgtga ggcgctactg gcgcgtgagc aagaatactt cactcgcgct
    14101 gcggggggca gggggacgga gctttgcgac gcatgcacag aatgcacctc tgttctatgt
    14161 tggcggtctg ccgtactgga tcaactatga ttacgagaac gtgcaatcca gccaactggt
    14221 gaacgacgcg ttttcgcagt ttgtattccc gctgcggggc actaattact acgagttgag
    14281 aggccgaagc ttcgcgattc tgaatgcgga ataccgattt cccctgatat atttccttgc
    14341 gacgggcccg ctcaactttg tgctcagcaa cgtcggcggc gtcgtgtttc tggatgtcgg
    14401 ccggacatgg gaggacccga cgcaacccag cgacccgaac atagcccgtc atgcgcaggg
    14461 tcccctcggc ggatacggct acgggatgcg gatcaacctc ggtgtgctgg ttctgagaat
    14521 ggacgtagcg tggcgaacag acctgaggga aacgatcggc agcgcgaggt attactggtc
    14581 tttcggctac gatttctgac acaccggccc gccgagagca atcaggtgga acagcatcgc
    14641 gacgccgatg tgaacctttc cctgtgattc cgcaagaggc atctccgtgg aagacagcgc
    14701 tcgaacgcct gcgcactttc tgtctcccga tctcccctct gtgacaagcc gccatgcaga
    14761 ggtgttgcgg cgtatccgcg tagtgctggt tgaaccgaaa taccccggga acatcgggtc
    14821 ggtggcacgc gcgatgacgg tgtgcgggct tacccgaatg cacctcgtca acccggttcc
    14881 gcgctggcgg gaatccgatg aggcgtggac tttcgcctgc gggtcgcgtt ctgttctgga
    14941 gcaggcgacc gaagcaaaga acctggaaga agcgctgcgc ggagccacga ccgtagttgg
    15001 cacgacgcac cggatgggaa gaggccgggg ccctctcctg cctccgcgaa aactggcgga
    15061 acatttgccg gggatgctct ccaaaggcga ggtggccctt gtgttcggac gggaagacaa
    15121 agggctttcg aatgacgaac tggcgttatg ccagatgggt gttcacatac cgacggcctc
    15181 tacctatccg tccttgaacc tttcccacgc cgtaatggtt gttgcgtacg agtgtttcta
    15241 cgcggtagtg aaggcggggg atgagaacgt gcaggtgagc ggggtttcgt acccggaaat
    15301 cgtggctctt tcggaaagaa tcgcacatct gaccgcgcgg actggctttc gtcaccggcg
    15361 gggagaagtg ggctttgaac gcagcctgcg gcgagccata gaacggagta gtctgagtgc
    15421 ccgcgacatt gctctcgcgc atcttgtgtg cagacaagtc gagcagtata tcgaccgcct
    15481 ggaggagcag attcgcaacg agcgggaaac gtcgaactga tcgacgacaa tggcaaaaat
    15541 gaaacgcggc gaggacagcg gaagtcgctc cgcgtcctcg ccgcgtgcgc caaactcaga
    15601 cactacaggg cagcgttccc cgattcgccg gtgcggatcc tgattgcgtc atctacagtt
    15661 gagacgaata tcttcccgtc gccaaccttt cccgtacgcg ccacccgcaa gagcgcattt
    15721 acgaccgcat caatctggtc atccaggacc actgcctcaa tcttgacttt gttcacgaac
    15781 agaacctgat actccgcacc gcgatagaac tcggtgtgcc cacgttgatg gccaaaacca
    15841 cgagcctcga gaaccgacag gccctttaca ccggcatctg tcagcgcggt gcggacgtcc
    15901 tcgaccagat ggggacggat gaatgcctcg actttcttca cgatctgtct ttcccgtttt
    15961 gttcctgttt gccgtgcgca ttacaggtga taggcggttt ccccgtgctg tgtcaaatcg
    16021 aggccctcac gctcttccgc ctgggaaaca cgcacgccgc cggcaatgtc tatcagtttc
    16081 aagataatcc atgtcgccgc cgctgcatag gcagcagtcg ccaccacgct cagcgcctgg
    16141 accagaagcg gatcgacgtt gcccagcagc aagccgtctt tgccgtttgc attccagtac
    16201 gtgctggcga agactccggt cagcaacgcg ccaagggtac caccgacacc gtgcacgcca
    16261 aacacatcga gggagtcgtc gtacccgaat cgtggtttga ggcagatggc ccagtaacag
    16321 atcacacccg ccgcaagccc gattgcgagc gcggccatcg gtcctacaaa cccagatgcg
    16381 ggtgtcaccg ccacgagccc ggcaaccatc ccggaagcga ctcccagcac ggttaccttc
    16441 cctgtccgaa ttcgttcggc gaacgcccag gccattgccg cggcggccgc agcgatctgc
    16501 gtggtgacaa acgccaatgt ggcaagcgcg ccgcttgcga gagcactgcc cccgttgaaa
    16561 ccaaaccacc cgaaccagag catcccggct cccagcaccg tccagatgag gctgttagga
    16621 agtatctggt ctttcgggta tccaactctt ctgccgatgt aaatggccgc cacaagcgca
    16681 gagaagcccg aactcatgtg gacaaccgtc cctccggcga agtcgagagc acctaacttg
    16741 aacaaccacc cgtccgatgc ccacacccag tggcagaccg gtgcgtacac gacggttgtc
    16801 cagaggagaa cgaacgcaac gtatgcgcgg aatttcatgc gctccgcaaa agcaccggtg
    16861 ataagggcgg gggtgattat ggcgaacatc ccctggaacg cgacgaacgc gaggtgaggt
    16921 atgcttccca tcggctccac tccgaccccg ttgaggaagg cgtggctgaa tccaccgatg
    16981 aacgagttgc cctttgagaa cgatagcgag taacctatca ccacccagtg gatccccacg
    17041 agtcccattg caaacacgct gtgcatgatc gttccgagca catttttgct gcgcaccatt
    17101 ccgccgtaga acaacgccag accgggcgtc atgaacagaa cgagagctgt ggacgtgagc
    17161 atccaggcag tgtcgccgga gtcgatactc ccggaaccct ccgcaaatgc gggagggacg
    17221 atcccgagga gtagtgtaag gaatgccatg agccgcctgt tcatccgaac ctccaaatca
    17281 gaaacagaac ctcgccgaac ctctgctgcg tggggcattt cacctgtgcc tcccgctccc
    17341 ttattgacga tagccacggc gtgtttccgt gaaatcacag cggcgtttcg cgagtgtaaa
    17401 aaaacgatga agttttgcag acatggtggg tgttgcaggt gagacggaga tagtggcggg
    17461 agcaggagtt acgcgatacc gtcaagaatc aatgacgcga acacggaata ccagtaatcg
    17521 ggaacaaagg gcgcatgaag caacgcatcc agcgtataaa gggtcagttc cagccggtct
    17581 tccctgcgga attccgtggt gcctgtgtac ccttcccaga ggaacatcca atcgggatcg
    17641 ttcacgaaag tccgtttcgt gtagtacgcg caactcatgg ccccgtataa cggcacaagg
//
LOCUS       DAOTNG010000019        17352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3273_length_17352_cov_5.564260, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000019 DAOTNG010000000
VERSION     DAOTNG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_17352_cov_5.564260"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="P5179_01805"
     CDS             <1..195
                     /locus_tag="P5179_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRS93988.1"
                     /translation="TAWKDGSLVTNGGRVLGVTAVADDIRTAVGRAYRAVDHIHFDGM
                     HYRKDIAHRAIARLASSTLK"
     gene            208..687
                     /gene="purE"
                     /locus_tag="P5179_01810"
     CDS             208..687
                     /gene="purE"
                     /locus_tag="P5179_01810"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRS93989.1"
                     /translation="MGNEAKETGKTDVLILMGSSSDMPVMEKAAAQLQEFGLSSEIVV
                     SSAHRNPDRTAQIAREAQDRGVKVIIAGAGMAAALPGVVAAHTSLPVIGVPCASGPLN
                     GFDSLLSIVQMPPGIPVACVAINGAKNAGVLAAEIIATGDPEVAVKVAEFRRKQSTA"
     gene            complement(695..1381)
                     /locus_tag="P5179_01815"
     CDS             complement(695..1381)
                     /locus_tag="P5179_01815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS93990.1"
                     /translation="MVGTAKPERAFFAHPTAIVDEPCSIGAGTRIWHYSHIMKNAVIG
                     ENCVFGQNCNVDTGVVVGNNVKVQNNTSLYTGLIVEDNVFLGPSCVFTNVTNPRSEIS
                     RRGLYEKTILRRGCTIGANATILCGTTIGRYAFVGAGAVVTKDVPDYGLVVGNPARFR
                     GWISRHGLPLRTPDANGVYVCPESGLRYKEVAPGVIRCLDLDEEAPMPEEMRVGSKSY
                     DAIVHPERPA"
     gene            complement(1391..2353)
                     /locus_tag="P5179_01820"
                     /pseudo
     CDS             complement(1391..2353)
                     /locus_tag="P5179_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(2473..2559)
                     /locus_tag="P5179_01825"
     tRNA            complement(2473..2559)
                     /locus_tag="P5179_01825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2523..2525),aa:Ser,seq:tga)
     gene            complement(2573..3019)
                     /gene="nrdR"
                     /locus_tag="P5179_01830"
     CDS             complement(2573..3019)
                     /gene="nrdR"
                     /locus_tag="P5179_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRS93991.1"
                     /translation="MRCPFCGKLEDKVVDSRASRDGATIRRRRECLVCSARFTTYEVV
                     ESVPVTVTKRDGRKEVYDRAKLQRGIELACAKRPVTQEQIERLVDEIELELFHGQTRE
                     VKSSRLGDMVLQRLEQLDKVARLRFASVFKDFETPGAFIDELKLFG"
     gene            complement(3029..3484)
                     /locus_tag="P5179_01835"
     CDS             complement(3029..3484)
                     /locus_tag="P5179_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRS93992.1"
                     /translation="MKIAFASDHAAFDLKNGVLPYARALGHEVVDFGCATKEPCDHSD
                     FSIPVAEAVARGEFDGAVLACGSGQGMTIGANKVDGIRATLCLTPEAARLARAHNNSN
                     ILVMAGRSLTPAEGQAIFEAWVSTPFEGGRHARRMDKIAEYERQRCAKR"
     gene            complement(3498..5114)
                     /gene="serA"
                     /locus_tag="P5179_01840"
     CDS             complement(3498..5114)
                     /gene="serA"
                     /locus_tag="P5179_01840"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRS93993.1"
                     /translation="MTEFLHHKVLISDDVHEICKTRLEAAGIEVDYRPGIKGAELLAA
                     LKDCTGLVVRSATRVTGEIIAAGPKLQVIGRAGVGVDNIDVEEATHHGIAVLNTPEGN
                     TTSAAEHTIGMMMALSRNIPQACAAMKAGKWSRSAFTGTEVRGKVLGVIGLGKIGREV
                     ARRARGLDMNVVCYDPMISPAAAEATGAQLLPLDAVLEQADYLTLHIPSNANTHHLLD
                     ADRLNKCKTGVRIINCARGEIIDTDALLDALNSGKVAGAALDVFENEPPDMTAPIIQH
                     PHTIVTPHLGASTAEAQHLVAEQIGQLVAEALTTGTIRNAINMFPMDRQTYERAKPFL
                     RLAERLGSLQAQVCDGQPVSLTLEYFGDVLEHPTGLLTASVLVGMLKQLESGTVNLVN
                     APVLAQERGIRVDEMRRTGDDDYNNVVTVTYQTRLPSGETLTRRIAGSVFGEDSPRVV
                     RIDGHSCDVIPEGRLLIMWNRDVPGVIGEVATVLGTHNVNIAFGSYGRDVPGGLAVMV
                     YVLDSDIEESTVDTLRKLKNLHTVKYARLA"
     gene            complement(5125..6270)
                     /locus_tag="P5179_01845"
     CDS             complement(5125..6270)
                     /locus_tag="P5179_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRS93994.1"
                     /translation="MKTRLYTPGPTMVPEQVSLDMAAPIIHHRTPEFSKVLQSCHEGL
                     QYLFQTKNPVLMLTCSGTGAMEGAVVSVLSRSTLTLVVRGGKFGERWGEICAAYGVPF
                     VPLDIPWGESITAEQLEEALAANPGVRAVCLTQSETSTGALNDIKTLAAVVKKRNALC
                     LVDSITGCGVHELRVDDWGIDVAVTGSQKGLMLPPGLAFAALSDEAWEAVKRTDLPSY
                     YLSFAKHRKSWEKTTTPWTPAITLLTGLARALEMIRSEGLEKLWERHQNMAEAMRQAV
                     RTLGLPLFPRVPANVQTVIALPEGLEYSRLSKQLFNVYGMKTAGGQDQLTGKILRIAH
                     LGYLDDGDIVAGVSFLERALADVGWKVNSGEAVAAAQRVLARTPAAQ"
     gene            complement(6287..6916)
                     /gene="yihA"
                     /locus_tag="P5179_01850"
     CDS             complement(6287..6916)
                     /gene="yihA"
                     /locus_tag="P5179_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRS93995.1"
                     /translation="MRVVSAEFVRSVVDLNELPAGRMPEIAWAGRSNVGKSSLINALT
                     GRKGLARTSNTPGKTREINFYVIDSSWHLVDLPGYGYVAAAESDRVRWQALIEPYLER
                     RKQLCGVAVLVDSKVGPTDLDQTMIEWLTRRGRNFAVVISKADRISDRHAADLAESLR
                     GTLPETAPVLITSAKQKQGLRELEGWILERLSGWRKETPVRATWHSETV"
     gene            complement(6943..8196)
                     /gene="clpX"
                     /locus_tag="P5179_01855"
     CDS             complement(6943..8196)
                     /gene="clpX"
                     /locus_tag="P5179_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRS93996.1"
                     /translation="MATRKREEELRCSFCNRGADQVKRLITGPNVHICDECIQMCNEI
                     LNDEKRTRPALQDKPLPKPAEIKRHLDEYAIGQERAKRILSVAVYNHYKRIRSAGANP
                     ENVELDKSNILLVGPTGTGKTLLAQTLSRILDVPFCIADATVLTEAGYVGEDVENILV
                     RLLQAADYDVARAEIGIVYLDEIDKVARKSANPSITRDVSGEGVQQALLKVLEGTISS
                     IPPKGGRKHPEQPLIQINTKNILFICGGAFEGLETIIQERIGRKYIGFNAPPESARKK
                     EASSILHHAEPDDLLKFGLIPELIGRLPVVAALDELDDEALMRILLEPKNALVRQYQH
                     LLGMENVRMRFEDDALKFIVDEARRRGSGARGLRAVIESAMVDVMFEIPSLSGISEVV
                     LTVDPAARKLKSLYLPDDGLRKQIA"
     gene            complement(8232..8834)
                     /locus_tag="P5179_01860"
     CDS             complement(8232..8834)
                     /locus_tag="P5179_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRS93997.1"
                     /translation="MNLAPMIIERDGRGERAYDIWSLLLKKRIVFLGTAIDDAVSNLV
                     VAQLLFLQSEDAKTPIEMYINSPGGSVSAGLAIYDTMKFIDAPVHTVCIGLAASMGAI
                     LLAAGEKGHRTALPHARIMIHQPWAGGIQGQATDIAIQAEEILKTRNRLNELISMDTG
                     RSLEDIARDTDRDKFFSAEEAKEYGIIDSVTTKQIAKIPA"
     gene            complement(8901..10172)
                     /gene="tig"
                     /locus_tag="P5179_01865"
     CDS             complement(8901..10172)
                     /gene="tig"
                     /locus_tag="P5179_01865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRS93998.1"
                     /translation="MKLEVTETAPCSRRINIEVEPDAIEERITSTVKEYRRTRTIPGF
                     RPGKATDSVIRRWFGKDIRAQVIRDLMEESWQKALEENKLQPVDQAQIDSVNAEPGEP
                     LRFSGTVEVKPQFDVQDYKGLPAIRPVHTVTEEDVTRQVNFLRERRATETPVERPAQN
                     GDVVVIDLQKLDETGVPLIGQKQDNLRWVLGGTGSVSKDLDEQIVGMAQGDTRNVTFH
                     YREDFYDPNRAGKEEKASITLKEVRERIVPEITEDFLKELGEYATENDLRKDLEADIK
                     RRLDALAKQQVHVGLKSALVERNQIEVPETLVQRLLTRMFQQHKQQGHEHDEDEFRAE
                     HREDALREVRASLLLERIAAQESIVVPDEDVDARIARMAASMGTSVRNLRDYMINNGQ
                     YEQIRYELQEEAVLNFLEEQAAIEESPVPKK"
     gene            complement(10275..10356)
                     /locus_tag="P5179_01870"
     tRNA            complement(10275..10356)
                     /locus_tag="P5179_01870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10320..10322),aa:Leu,seq:tag)
     gene            complement(10511..11515)
                     /locus_tag="P5179_01875"
     CDS             complement(10511..11515)
                     /locus_tag="P5179_01875"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS93999.1"
                     /translation="MNFRWLAYSGLAFFLVASRVEAFGSGDDSGAAGFKSLFLVATSR
                     SAALGGADCALSANPLAAAGNPSASGGIASRTAGIAVTDYVLDVLPVVAYGAYPARHG
                     VWSVVLGNVSFGEFQRTNETAAEVGTFGANDLSLRVGWAGPLRLGIVGGVSLGWIRST
                     IDAYSASALLMNVGLQWHAPNQQTVVAVSAENLGGALSSYVGGSQGLKDDVPTEFHAG
                     VSHRPAHFPLPLLLVSDFTLPRDNEVSASVGAEIRPFEPLFIRVGYASLVRYRSNTDS
                     DGTHKSGFAFDDRHGSAFGGSGLAAGFGVVHKSLSLDYAYSLAGAFGGVHRVAAAWAW
                     "
     gene            complement(11512..12987)
                     /gene="gatB"
                     /locus_tag="P5179_01880"
     CDS             complement(11512..12987)
                     /gene="gatB"
                     /locus_tag="P5179_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRS94000.1"
                     /translation="MSNYRVVIGLEIHLQLATKTKVFCGCSNEFGGEPNTHSCPVCLG
                     MPGVLPVFNRQVLEYALRLACALGSRVSERCRFVRKNYFYPDLPKGYQISQFHECLAY
                     GGAVEIDVSGTTKRICLDRIQIEEDAGKSLHSEDSHMEDTLVDVNRCGTPLLEIISKP
                     TPGSPDCVYDPSAYLNSPDEAIAYWVKLRQLTRYLGISECNMEEGNMRADANISIWDE
                     ARGSFGTKTEIKNLNSFKFAHRALTQEIARHIEILSAGGKVKQETMLYDSSTDTVAPM
                     RSKEEAHDYRYFPEPDLVPVVVTEEFLAQVRKNVPELPDARKKRFVSEFGLNQDAADL
                     LSSERALADYFEGVVKSGAEPRTAANWIMGDVLRETNARKMDVSVFPVSPPRLAELIA
                     LVVSGTINQNGAKDVFAEMVDSGKQATQIVEERGLAQISDKTALAGIVAEVMGRFPGE
                     IQRFRAGEVKLMGFFVGQVMKATKGRANPALVNDLVREYLS"
     gene            complement(13015..14463)
                     /gene="gatA"
                     /locus_tag="P5179_01885"
     CDS             complement(13015..14463)
                     /gene="gatA"
                     /locus_tag="P5179_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRS94001.1"
                     /translation="MNNPKFTAAALVPQILEGSVRATDVLDTVLATIREQEPRVHAFL
                     QVTEDLARSQAEQVDRKIAEGKPVGPLAGIPVGIKDNLCVRDGRTTCASKILEPFVAP
                     YDATVISRLRDADAVFVGKTNLDEFAMGSSTENSGFGVTRNPIDLERVPGGSSGGSAA
                     AIAAGMCVLALGSDTGGSIRQPASFCGIVGLKPTYSRVSRFGLVAFASSLDQIGPMAQ
                     TVRDAAMLLNVIAGADAHDSTCSPDPVPDYLEGLEDGVRGLRLGVPREYFGEGLAPEI
                     KTGLTQVIHQMEREGAKLVDISLPHAPYAIGTYYLIATSEASANLARYDGVKYGFRAK
                     DVKNLGEMYRRTRSEGFGAEVKRRILLGTYALSSGYYDQFYVKAQKVRTLISQDFASA
                     FAKVDAILTPTTPDTAFKLGEKTSDPLQMYLEDIYTVSANLAGIPGISVPAGRDSSGL
                     PFGMQVLGPQFGEPVVLRVARAVEMLRPGGAG"
     gene            complement(14521..14703)
                     /gene="tatA"
                     /locus_tag="P5179_01890"
     CDS             complement(14521..14703)
                     /gene="tatA"
                     /locus_tag="P5179_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRS94002.1"
                     /translation="MIGGWEWVIIIVVVLLVFGARRIPELGKSLGQGIRSFKEELAGG
                     KQRKDEPKDDSSSGQS"
     gene            complement(14729..14998)
                     /locus_tag="P5179_01895"
     CDS             complement(14729..14998)
                     /locus_tag="P5179_01895"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HRS94003.1"
                     /translation="MPRYRSTGVLFASLLIGLLGATVLSAALSLFVGPASVVHRVLIN
                     SFTYDSGVLVLNLIVVTISFGITLNINLLTILGLMSAFYWWKYRL"
     gene            complement(14999..15781)
                     /gene="pssA"
                     /locus_tag="P5179_01900"
     CDS             complement(14999..15781)
                     /gene="pssA"
                     /locus_tag="P5179_01900"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRS94004.1"
                     /translation="MMPLVTQALPSVMTLGSLFCGLLAAIKAMEGTEEAFVTAAWLII
                     AAGLFDGLDGKVSRIFKTQSHFGVELDSIVDVCSFGFAPALLIYQFILLNASGFSGIA
                     FPVAFVFLACGALRLARFNAQLKGFDKAAFKGVPIPAGAGMVASFVVFSRSEFIIDLG
                     IDWVLPFLAFGMALLMVSTIRYDKMPRFSWHGPTSERVRLVVMIAFLVIAVWYPAEAF
                     FSFGVYYVLYGLVRALGNAFVRHPAVAPGQPSLERVKEGSRR"
     gene            complement(15778..16416)
                     /locus_tag="P5179_01905"
     CDS             complement(15778..16416)
                     /locus_tag="P5179_01905"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRS94005.1"
                     /translation="MAKEGLPFVLGGVCAGILLLAAWHGLRWEPLRILGILALVFGAF
                     SLFFFRDPRRTPPAGEGLVLSPADGKVVEIVQEDDPYVGTNAIRVSIFLSVLDVHVNR
                     NPISGSVTAMVYRPGKYLMAFNEKASADNEQTHIAIKGERGTVAFKQIAGFIARRIVC
                     RLKVGDTVRAGDRCGLIRFGSRVDVILPPDASIRVAVGQRAVGGKTVIGVLP"
     gene            complement(16409..>17352)
                     /locus_tag="P5179_01910"
     CDS             complement(16409..>17352)
                     /locus_tag="P5179_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRS94006.1"
                     /translation="PFADDPMWQFFFPELRQEFVRRVQSMGSGFIVSPDGMILTNEHV
                     IRGATQIVVNLPDGRSFDAKIFGEPDEQADIAVLKIDAQNLPTAPLGRSDDLMIGEWA
                     IAIGNPFGFLIRDAHPTVTVGVISALNRDFSPSSAGGRTYRDMIQSDASINPGNSGGP
                     LVNSEGYVIGINTFIFTQGGGSEGVGFAIPIQKARQVLDDIVQFGEVRHNFWTGIHIQ
                     DVNRWIAESLGLQSDRGAIITNVEPNSPGAKAGLQRGDVIISVNGKAIASDRDVRDEF
                     LGAVVGQRVELRIIRNGRELTVTLVLEEIPRNRRDRG"
BASE COUNT         3503 a         5256 c         4847 g         3746 t
ORIGIN      
        1 acggcatgga aggatggttc cctggtgacc aacggggggc gcgttctcgg cgtcaccgcc
       61 gtggcagacg acattcggac tgccgtaggt cgagcgtaca gggcagtgga ccatatccat
      121 ttcgatggca tgcactaccg gaaggatatc gcacaccgcg ccattgcgcg gctggcgagt
      181 tcaactttga aatgacgggg caaaccgatg ggaaatgagg caaaggaaac cgggaaaacg
      241 gacgtgctca tcctcatggg cagttcttct gatatgcctg tgatggagaa ggcggccgca
      301 cagcttcagg aattcgggct ttccagcgag atcgtcgtct cttcggcgca cagaaacccg
      361 gatcgcaccg cgcagatagc gcgagaagct caggatcgcg gtgtgaaagt tattattgcg
      421 ggtgccggca tggcggcggc gcttccggga gtagtcgccg cccatacaag cctgccggtg
      481 atcggcgttc cctgcgcgtc aggaccattg aacggattcg acagcctcct ttcgatcgtc
      541 cagatgccgc ccggcatacc ggtggcgtgc gtagcgatca atggggcaaa aaacgctggc
      601 gtcctcgcgg cggagatcat cgctaccggc gaccctgagg tagcagtgaa agttgcggaa
      661 ttccgcagaa agcagagcac agcgtaattt ggagctatgc aggtcgctcg gggtgtacta
      721 tggcgtcgta ggatttcgaa cccacgcgca tctcctccgg catcggggct tcctcgtcaa
      781 gatcgaggca gcggatcact cccggagcaa cttctttgta tctcagcccg ctttcagggc
      841 agacgtacac accgttcgca tcgggggtac gcaacggcaa cccgtgccgg ctgatccatc
      901 cgcggaatct tgccggattg ccgaccacaa gcccgtagtc gggcacgtct ttcgttacaa
      961 ctgcgcccgc gcccacaaac gcgtacctcc cgatcgtcgt gccgcaaaga atcgttgcgt
     1021 ttgccccgat tgtacagccc ctccggagga tcgtcttctc gtaaaggccc cgccgcgaga
     1081 tctccgaccg cgggttggtc acgttcgtga acacgcagga aggcccaagg aacacattgt
     1141 cctcaacaat gagccctgtg taaagcgagg tgttgttctg aaccttgacg ttattcccta
     1201 ccacaacccc ggtgtccacg ttgcagttct gcccgaaaac gcaattctcg ccgatcaccg
     1261 catttttcat gatgtggctg taatgccaga ttcgcgttcc cgccccgatc gagcaaggtt
     1321 cgtccacgat ggcagtagga tgagcgaaaa acgcgcgttc gggttttgca gtcccgacca
     1381 gagtcacagg tactcccccc ttctccaacg attcctggca cgtttcaaga acctcgagaa
     1441 ccgcgagcgc ttcccgaccg tccgtcagtg gatttgaccg ctcttcgatg catttcagga
     1501 aatgcctgca ttcattctcc aacggctcct catccgcaac gtcgatgtgt tctcgaggcg
     1561 ctttgatggc ggttggaaca tcgttcaccc attccacgcc gtgtgaatac gcgacaagtt
     1621 tctcggatga agtgtcatcg aacacaacca ttttccgcga cccgaccaca accaatcgct
     1681 gttccttgaa cgggtgcaac cagctgacga aaagatggcc gcgaacgccc tccgggaatt
     1741 ccagatcgct catcgtcacg tccgggacac ctttgttcag atatgccgag ccgtggcacg
     1801 agacgcgttc agggtagcgc ccgaccagcg caagtatcac ggagatatca tgaggcgcga
     1861 aactccagag gatgttctcc tcggtgcgta tcttcccgat attgaggcgg ttggaataga
     1921 cataacggat ttccccgagt tctcccgcct ccaccatcgt gcccagcgtg cgaatcgcgg
     1981 ggtggtaacg aagtatgtgg cccaccatga ggattctcga ggtgcgggct gctgcttcca
     2041 ccatcgtgcg gccatcggcg accgtcaacg cgagaggttt ctcgacgtac acgtctttgt
     2101 gtgcatccaa agccgccatt gcgtgtcgaa aatggtccgc cgccggtgtt gcgaacacga
     2161 ctgcgccaac gctcgtatcc gcgagaacct ggttcatgtc agacgtgaaa gaaacgccgg
     2221 ggtactcgcg ctcgatttcc tgctttttgg aggaaacggt gtcgcagacc gtgtggagcg
     2281 ctccgagctt gtgaaaaacc cgaaccaggt tcttgcccca gtagcccatt cctaccactg
     2341 cgatacggat catcaagctg cgctccaatc gaggcgtttc aaccgggaag taggtctcgt
     2401 tttgtggcgc ggggagtaag gagggagaaa aaggccgaaa cgcaaactcg caggggcctt
     2461 cgcaaccagt gctggcggag ggggtgggat tcgaacccac ggacctcgtc ggtcggcagt
     2521 tttcaagact gctgctttca accgctcagc cacccctccg cgtgaaggag actcatccga
     2581 atagcttaag ttcgtcgata aaggcgcccg gggtttcgaa atccttgaag accgaagcga
     2641 accggaggcg agctactttg tcgagctgtt ccagccgctg gagtaccata tctccgaggc
     2701 gcgaggactt cacctcccgg gtctgtccgt ggaaaagctc aagctctatc tcgtccacaa
     2761 gccgttcgat ctgctcctgc gtaacgggcc gcttcgcgca cgcaagctct atcccgcgct
     2821 ggagcttcgc gcggtcatac acctctttcc tgccgtctct cttcgtcacc gtgacgggaa
     2881 ccgattccac cacttcatag gtggtaaaac gcgcggaaca aacgaggcac tcgcgccggc
     2941 ggcgtatggt tgctccgtcc cggctggcgc gtgagtcgac taccttgtcc tcgagttttc
     3001 cgcagaaagg gcatcgcatc gtgctcggtt acctctttgc acaccgttgg cgttcgtact
     3061 cggcgatctt atccattcga cgggcatggc gaccaccctc aaacggcgta gatacccaag
     3121 cttcaaaaat cgcctgccct tcggcggggg tcaaagacct ccccgccata acgagaatgt
     3181 tgctgttgtt gtgggctctg gcaaggcggg ccgcttccgg ggtgaggcat aacgtcgccc
     3241 gaataccatc tactttgttc gccccaatcg tcattccctg accgcttccg catgccagaa
     3301 cagcgccatc aaactcaccc cgtgccactg cttccgcgac cggaatgctg aagtcggagt
     3361 gatcgcatgg ctcctttgtg gcgcacccga agtcaacgac ctcgtgaccg agtgctcttg
     3421 cgtacggtaa tacaccattt ttcaagtcga acgcagcatg gtccgatgcg aacgcaattt
     3481 tcatccgaac agcccaatca cgccagtctg gcgtacttca ctgtgtgcaa gtttttcagc
     3541 ttgcgcaaag tatccacggt gctttcttct atgtcgctgt cgagcacgta gaccatgacg
     3601 gcgagtccgc cgggtacgtc tcgcccgtac gaaccgaacg cgatgttgac gttgtgcgtg
     3661 cccagaaccg tggcaacctc tccaattacg ccaggcacat cacggttcca catgatcagc
     3721 aaccggcctt ccgggatgac atcgcacgag tgaccgtcaa tacggacgac gcgtggcgaa
     3781 tcctcgccga aaacgctccc cgcgatacgc cgcgtgagcg tctccccgga cggaagccgc
     3841 gtttgatacg tcaccgtaac aacgttgttg tagtcatcat caccggtccg tcgcatttcg
     3901 tccacgcgga tgccgcgttc ctgagcgagc actggagcat tgacgaggtt tacggtcccc
     3961 gattcgagtt gcttgagcat tcctaccagc accgacgccg tgagaagccc cgtcgggtgc
     4021 tccagaacgt ctccgaagta ctccagtgta agggaaaccg gctggccgtc gcagacttgc
     4081 gcctgcaacg aaccaagacg ctccgccaga cgcaggaacg gtttggcgcg ttcgtaggtc
     4141 tgacggtcca tgggaaacat gttgatggcg ttcctgatcg ttcccgtggt aagcgcctca
     4201 gccaccagtt ggccgatttg ctccgcaacc agatgctgag cttcggcagt ggaagcaccc
     4261 agatgcggcg tcacaatggt gtgcggatgc tggatgatcg gcgccgtcat gtccggtggc
     4321 tcgttctcaa atacatccag cgcggccccc gccactttgc cggaattgag tgcatccaga
     4381 agcgcgtccg tgtcgatgat ctctccgcgc gcacagttga ttatccgaac gccggtcttg
     4441 cacttgttca gcctgtcagc gtccagaagg tgatgcgtgt tcgcgttcga cgggatgtga
     4501 agcgtcagat agtccgcttg ttcaagcacc gcatccagcg gcaacaactg cgctccggtc
     4561 gcttctgctg ccgcaggaga gatcatcggg tcgtaacaca cgacattcat gtcgagaccc
     4621 cgtgcacgcc gggcaacttc gcgaccgatt ttcccgaggc cgattacacc cagaaccttg
     4681 ccgcgaacct ccgtgccggt gaacgcggat cgactccatt tcccggcctt catggctgca
     4741 caagcctgtg gaatgttccg tgacaacgcc atcatcattc cgatggtgtg ctcggcggcg
     4801 ctggtggtgt tcccctcggg ggtgttcaga acagcgatcc cgtggtgcgt cgcttcttcc
     4861 acgtcaatgt tgtccacccc aactccggcg cgtcctatga cctgcaactt gggacctgct
     4921 gcgatgatct ccccggtgac ccgtgtcgcc gagcgaacga ccagacccgt gcagtccttc
     4981 agtgccgcca gtagttcagc gccctttatg ccgggacggt agtctacttc tatccccgcc
     5041 gcttcgaggc gcgtcttgca gatttcgtgc acgtcatcac tgataagcac tttgtggtga
     5101 aggaattcag tcatggaacc ggcctcattg tgctgcgggc gtacgggcga gaacgcgttg
     5161 agcggccgca accgcctcac ccgaatttac cttccatccg acatcggcaa gagcacgctc
     5221 caggaacgaa acgccggcca cgatgtcgcc atcgtcgagg taaccgagat gcgcgatgcg
     5281 caggatcttt cctgtgagct ggtcttgtcc accggccgtt ttcatcccgt agacgttgaa
     5341 cagctgcttg ctcaaacggc tgtactccag tccttccggc agcgcgatta ccgtctgcac
     5401 gttcgcgggg accctgggga aaaggggaag gccgagcgtg cgcaccgcct gtcgcatggc
     5461 ttccgccatg ttctggtgcc gttcccagag cttctcaagc ccctccgacc gaatcatttc
     5521 cagcgcgcgc gccagtcctg tgagcaacgt gatcgcgggc gtccagggtg tcgttgtctt
     5581 ctcccacgat ttccggtgtt tggcgaaact gagataatac gaggggaggt cggtccgttt
     5641 gactgcttcc caggcttcgt cgctcagcgc tgcgaacgcc agtcccggcg gcaacatgag
     5701 acccttctgg gatcccgtca ccgcaacgtc aattccccaa tcatccacgc ggagctcgtg
     5761 aaccccgcaa ccggtgatgc tgtccacgag acacagggcg ttcctctttt tcacgactgc
     5821 ggcaagcgtt ttgatgtcgt tgagcgcacc ggtggatgtc tcactctgcg tgaggcagac
     5881 tgcacgaact cccggattcg ccgccaacgc ctcttcaagt tgctctgcgg taatgctttc
     5941 accccaggga atgtccagag gaacaaacgg aactccgtat gccgcacaga tttctcccca
     6001 gcgctccccg aatttgcctc cccggacgac cagcgtcagg gtggaacggg acaggaccga
     6061 tacgacagct ccctccatcg ctcccgtgcc gctgcaggtg agcatgagca cggggttctt
     6121 tgtctggaac agatactgaa gcccctcatg gcagctctgc aacaccttgg agaactccgg
     6181 cgtccgatgg tgtattatcg gcgccgccat gtcaagcgat acctgttcgg gtaccattgt
     6241 tgggccaggg gtatacaggc gcgttttcac ggtcgttccg tctccctcat acggtttccg
     6301 aatgccatgt tgcccggacc ggagtctcct tccgccatcc agagagccgt tccaggatcc
     6361 atccttccag ttcccgcaag ccttgtttct gcttggcgga ggtaatcaga accggggccg
     6421 tttcaggcaa cgtgcccctc aacgattccg caaggtcggc ggcgtggcgg tccgaaatcc
     6481 tgtccgcctt tgagatcacc accgcaaagt tcctgcctcg cctcgtcaac cattcgatca
     6541 tcgtctggtc taagtcggtg gggccgactt ttgaatccac aagcacagcg acgccgcaca
     6601 actgcttccg gcgctcaaga tacggctcaa tcaacgcttg ccagcggact ctgtctgatt
     6661 cggcggcagc gacatacccg tagcccggaa gatccaccag atgccagctc gaatctatga
     6721 cgtagaaatt gatctcgcgc gtttttcccg gcgtgttcga cgttcgcgca aggccttttc
     6781 tgcctgtcag cgcattgatg agcgacgatt tccctacgtt ggatcgcccg gcccaggcaa
     6841 tttcgggcat ccgcccggca ggcagctcgt tgagatcaac aacgcttctg acgaactccg
     6901 cagagacaac gcgcatcaca taaccaggtc gaagagccgc tctcaggcga tctgtttgcg
     6961 cagaccgtca tccggaagat acagcgattt gagcttccgc gcagcaggat ccaccgtcaa
     7021 gaccacctca ctgatcccgc tcaacgatgg gatttcgaac atcacgtcca ccattgcgga
     7081 ttcgataacg gctctcagcc cacgcgcgcc acttccccgt ctccgggcct cgtccacgat
     7141 gaacttcagc gcgtcatcct caaagcgcat gcggacgttt tccatcccga gcaggtgctg
     7201 gtactgccga acaagcgcat tcttcggttc cagcaggatt cgcatcagcg cctcgtcatc
     7261 cagttcatcc aacgcggcga caaccggcag tcgaccgatc agctccggga tcaagccgaa
     7321 tttcaggagg tcatccggtt ctgcgtgatg caggatcgaa gaggcttcct tctttcttgc
     7381 ggattccggg ggagcattga aaccgatgta tttccgcccg attctctcct gaatgatcgt
     7441 ttccagcccc tcaaatgctc ccccgcagat gaagagaatg ttcttcgtgt tgatctggat
     7501 caggggttgt tccgggtgtt tgcgcccccc cttcggtggt atgctgctga tagttccctc
     7561 aagcactttc agcagagcct gttgtacgcc ttcacctgac acatcgcgcg ttatcgaggg
     7621 gtttgccgat ttcctcgcca ccttgtcgat ttcgtcgaga tacacgatcc cgatttccgc
     7681 tcgggcgaca tcgtagtcag ccgcttgaag aagccgcacg aggatgtttt caacatcctc
     7741 accgacataa cccgcttccg tcaggacggt cgcgtctgcg atgcagaacg gcacgtcgag
     7801 aatgcgggaa agcgtctggg caagaagtgt tttgccggtg ccggtaggtc cgaccagcag
     7861 gatattgctt ttgtccaact cgacgttttc ggggttggcg ccagcggaac gaatgcgttt
     7921 gtagtggttg tacaccgcca cgctgagaat tcttttggcg cgttcctgtc caatcgcgta
     7981 ttcatccaga tgccgtttga tttccgcggg tttcgggagc ggtttgtctt gcagcgccgg
     8041 ccgggtacgc ttctcgtcat tgagaatttc gttgcacatc tgaatgcatt catcgcagat
     8101 gtgcacattc gggccggtaa tgaggcgctt gacctggtcc gcgccgcggt tgcagaagga
     8161 gcaacgcagt tcttcttcgc gctttcgtgt cgccactcgt tttgtactcc cagaagctga
     8221 actgcccggt ctcaggcagg gatcttcgcg atctgtttgg tggttacgga atcgatgatg
     8281 ccgtactcct tcgcttcttc cgcgctgaag aatttgtcgc ggtcggtgtc tctcgctatg
     8341 tcttcaaggc tgcgtccggt atccatcgag atcagctcgt tcaggcggtt ccgcgttttc
     8401 aggatttcct ccgcctgaat ggcgatgtcg gtcgcctgcc cctgaatgcc gccggcccag
     8461 ggttgatgga tcatgatccg cgcatgcggc aatgcggtcc ggtgtccttt ctcgccggct
     8521 gcaagcagaa tggctcccat gctggctgcc agaccaatgc acaccgtgtg aaccggggca
     8581 tcgatgaatt tcatcgtgtc atatatcgca agccccgcgg aaacgcttcc gccaggactg
     8641 ttgatataca tttcgatggg tgttttcgcg tcttctgact ggagaaacag gagctgcgcg
     8701 acgacaagat tggacacggc atcgtctatc gcggttccca ggaaaacgat tcgttttttg
     8761 agaagcaggg accagatgtc gtaggctcgt tcacctcgac cgtcacgttc tattatcatg
     8821 ggcgccagat tcatggtttt cactccttgc agaaacgggt actcgttcgc gcgtgttccg
     8881 gattgctgtc cggtttctca tcactttttc ggaaccgggc tttcctcaat cgccgcttgc
     8941 tcctcgagga agttcaggac tgcttcctcc tgcaactcat accggatttg ctcgtactgt
     9001 ccgttgttga tcatgtaatc cctgaggttg cgaaccgaag ttcccatgga cgcggccatc
     9061 cgggcaatac gagcgtccac gtcctcatcg ggtacgacga tgctctcctg cgcggcaatc
     9121 cgttcaagga ggagcgaagc ccggacctct cgcaacgcat cctcgcggtg ctccgcgcga
     9181 aactcgtcct catcgtgctc gtgtccctgt tgcttgtgct gctgaaacat ccttgtaagc
     9241 agtcgctgga caagggtctc cgggacttca atctggtttc tttccacaag tgcgcttttc
     9301 agtccgacgt gcacctgctg cttagcgagc gcgtcgagcc ggcgcttgat gtccgcctcc
     9361 aggtctttcc ggagatcatt ttccgtcgcg tattcgccga gttcctttag gaagtcttca
     9421 gtgatctcgg gtacgattcg ctcacggacc tctttgaggg ttatggacgc tttttcctct
     9481 ttgccggcac ggttcggatc ataaaaatcc tcgcggtaat ggaacgttac gttccgcgta
     9541 tcgccttgtg ccattccgac gatctgttcg tccaaatcct tgctcaccga tccggttccg
     9601 cccagaaccc atcggaggtt gtcttgtttc tggccaatga gggggacacc cgtttcatcg
     9661 agtttctgga gatcgataac cacgacgtcg ccgttttgcg cagggcgttc gaccggagtc
     9721 tcggttgcgc ggcgttcacg caggaaattg acctgtctcg tcacgtcctc ttcggtcacc
     9781 gtgtgaaccg gccggatcgc cgggagccct ttgtaatcct gcacgtcgaa ttgtggcttc
     9841 acctcgacag ttccggaaaa acgcaacggt tcccccggtt ccgcattgac cgaatcgatc
     9901 tgcgcctggt ctacgggctg aagcttgttc tcttccagag ctttctgcca cgattcctcc
     9961 atgaggtcgc ggatgacctg ggcgcggatg tcttttccga accacctcct gatgacggaa
    10021 tcggttgcct tacccggtcg gaaaccaggg attgttctgg tacggcgata ttcctttacc
    10081 gtggaggtta tgcgctcttc tatggcgtcg ggttcgactt cgatgttaat gcgtcggctg
    10141 cacggtgcgg tttcagtgac ctcaagtttc atcgggttcc tcaattcgcg ctggcgccgt
    10201 ggccagtcgg gtgccagtga tgaaggatcg tcgcggggcg aacgccttcg ggaaagggtg
    10261 tgctgcgcga gggatgcgag aggagggact cgaaccctca cgccgaaagg caccagatcc
    10321 taagtctggc gcgtctgcca attccgccac tctcgcgttc atggcccgca cgcaggttac
    10381 ctgcgagcag attgctcaaa atattaaaga taccactgcg gcgtgaggag gtcaacgaag
    10441 cgcgatgaac catgcaccgg ttcgcgcgcg ccgcgtccgt gcagaatgcg cgcaaatcag
    10501 tgcaacttga ttaccacgcc cacgcggcgg caacgcggtg aacaccgcca aacgcgcccg
    10561 cgaggctgta ggcataatcc agagaaaggc ttttgtgaac gactccgaag cctgcggcga
    10621 gccctgatcc tccgaacgca gacccgtggc gatcatcgaa cgcgaatccc gatttgtgag
    10681 tgccgtcact gtctgtgttg gagcggtacc gtacgaggga agcataccca acccggatga
    10741 acaggggctc gaaagggcga atttccgcgc ctacggaagc ggaaacttcg ttatcccgcg
    10801 ggagcgtgaa atcagaaacc agcagcaggg gcaacgggaa atgggccggg cggtgagaaa
    10861 ccccggcgtg gaactccgtc ggaacatcat ctttcagccc ctgtgaaccg ccgacgtagc
    10921 tgcttagagc acctccaagg ttttccgcgg acaccgcaac gacagtctgc tgattcggcg
    10981 cgtgccactg gagtccgacg ttcatcaaca gcgcagaggc cgaataggcg tcgatcgtcg
    11041 atcgtatcca accgagtgaa acaccgccga caatccccaa ccgcagcggc ccggcccacc
    11101 cgactctgag ggagagatcg tttgccccaa acgtgccgac ttccgcggcc gtctcattgg
    11161 tccgttggaa ctctccgaac gagacgtttc cgaggaccac agaccagacg ccgtggcgag
    11221 cggggtacgc gccgtacgca accaccggca gcacgtcaag cacgtaatcc gtcaccgcga
    11281 taccagcggt tctggaggca attccgcctg aagctgatgg gttgcctgct gcggccagag
    11341 ggttggctga cagcgcgcag tccgcgccgc caagggcagc ggaacgactg gtggcgacga
    11401 ggaaaaggct cttgaaacct gcggcgccgc tgtcatctcc ggacccgaac gcttccaccc
    11461 gagaggcgac caggaagaag gcaaggccac tgtatgccag ccatctgaag ttcatgacag
    11521 atactctcgg accagatcat tcacaagcgc ggggttggcg cgccccttcg tggccttcat
    11581 cacctgaccg acaaagaacc ccataagctt gacttcgccg gctcgaaaac gctggatttc
    11641 tcccgggaat cggcccatga cttccgccac gatacccgcg agggccgtct tgtccgatat
    11701 ctgcgccaat ccacgctctt cgactatttg cgttgcctgc tttccagaat ccaccatctc
    11761 cgcgaacaca tccttggcac cgttctggtt gatggtacca gataccacca gggcaatcag
    11821 ttcggcgaga cgcggagggc tgaccggaaa gactgacacg tccatcttgc gtgcgtttgt
    11881 ttcccgtaac acatcgccca tgatccagtt tgccgccgtc cgtggctctg cgcccgattt
    11941 cacgactcct tcaaaatagt cggcaagcgc ccgttccgac gaaagaagat ccgccgcatc
    12001 ctggttgaga ccgaattcgg atacgaaccg cttcttcctc gcgtccggaa gctcgggaac
    12061 attctttcga acctgtgcca ggaactcttc tgtgacgacc acgggtacaa ggtccggctc
    12121 cggaaaatac cggtaatcgt gtgcttcctc tttggacctc atcggggcga cggtatccgt
    12181 cgacgaatcg tacaacatgg tttcctgttt tactttgccg ccggcgctca atatctcgat
    12241 gtgccgtgca atttcctgag tgagtgctcg atgcgcgaat ttgaaggagt tgaggttttt
    12301 tatttccgtt ttcgttccaa aggatccccg tgcttcgtcc cagatcgaga tatttgcgtc
    12361 agcccgcata ttgccttctt ccatgttgca ctcggagatt ccgaggtaac gggtgagctg
    12421 acgcagtttt acccagtatg cgattgcctc gtccggcgaa ttcaggtacg cggatggatc
    12481 gtacacgcaa tcgggggagc cgggagtggg tttgctgatg atttcgagca ggggcgtacc
    12541 acaccggttg acgtcaacca gcgtgtcctc catgtgcgaa tcctcgctgt gcagcgattt
    12601 tccggcgtct tcctctatct gtatgcgatc gaggcatatg cgcttggtag tgccggagac
    12661 gtcaatctcg accgcgccgc cataggcgag acattcgtgg aactgggaaa tctggtatcc
    12721 ctttggcaag tcagggtaga agtagttttt gcgcacgaaa cggcaccgtt cactcactct
    12781 ggaacccaac gcacacgcaa ggcgcaacgc gtactcaagc acctgtcggt tgaacacggg
    12841 gaggacgccc ggcatgccga ggcacaccgg acaggagtgg gtattcggtt cacctccaaa
    12901 ctcgttgctg cagccacaga acacctttgt cttcgtggcc agttgaaggt gtatttccag
    12961 cccgatcacg acccggtaat tcgacatgct cttcgtccat tcctcgtcac aagttcaacc
    13021 tgccccgccg gggcgcagca tttccactgc acgtgcgacg cgcagcacga caggctctcc
    13081 aaattgtggg cccaggacct gcattccgaa gggcaaccca gagctatctc tgccggctgg
    13141 aacggatatc ccgggaattc ccgccagatt cgcgctcacc gtgtaaatgt cctcgagata
    13201 catctgcagg gggtctgagg ttttctcccc gagcttgaat gcggtgtccg gcgtggtggg
    13261 agtaaggatg gcgtccactt tcgcgaacgc gctcgcaaaa tcctgtgaaa tgagggtcct
    13321 gaccttttgc gccttgacgt agaactgatc atagtacccc gagctcaacg cgtacgttcc
    13381 gaggagaatt ctccgtttta cctcggcgcc aaatccttcg gaacgggtcc gtcggtacat
    13441 ctccccgagg tttttgacgt ccttcgcgcg gaacccgtac ttcaccccat cgtaccgggc
    13501 gaggttggcg gacgcttcgg atgttgcgat gaggtagtag gtgccgattg cgtaaggtgc
    13561 atgggggagc gaaatgtcga cgagtttcgc gccttctcgc tccatctggt ggatgacctg
    13621 cgtgagcccc gtctttattt cgggagcgag gccttcgcca aaatactcac gcggtacgcc
    13681 gagacgaaga ccgcggaccc catcctccaa cccttcgagg taatctggga ctggatccgg
    13741 ggagcaggtc gaatcgtgcg cgtccgctcc tgcaatcacg ttgaggagca tggcagcatc
    13801 gcgcacggtc tgcgccatag gcccgatctg gtcaagggat gaggcgaatg cgacaagacc
    13861 aaaccgtgat acgcggctgt acgtgggttt gagacccact ataccgcaga agctggcggg
    13921 ctggcgaata gatcccccgg tatcgctgcc aagagcgaga acgcacattc ctgccgcgat
    13981 tgccgcggca gaccctccgc tggaccctcc gggcactcgt tcgaggtcta tcgggtttcg
    14041 cgtcacacca aacccggaat tctccgtcga cgagcccatg gcgaactcat ccagattcgt
    14101 tttcccgacg aatacggcat ccgcatcccg cagacgggaa atcaccgtag catcgtacgg
    14161 cgccacgaac ggttcgagga ttttggaggc acacgtagtg cggccatcgc gcacgcagag
    14221 attgtccttg atcccgacgg ggatgcccgc aagaggcccc accggttttc cctcggctat
    14281 cttgcggtct acctgttcag cctgtgacct tgcgaggtcc tctgtgacct gaagaaatgc
    14341 gtgaacgcga ggttcctgct cccgaatggt ggcgagcacg gtatccagga cgtcggttgc
    14401 ccgcaccgat ccttcgagga tctgaggaac aagtgccgca gcggtgaatt tcggattatt
    14461 caaaggatta cgcctgtgtt gagggctcag aacgttgact gacacaccac agaagcggga
    14521 ttacgattgc ccggagctgg aatcatcctt aggctcgtct ttccgctgtt tgccgcctgc
    14581 cagttcctct ttgaacgacc ggattccctg cccgagcgat ttgccaagtt ccgggatgcg
    14641 gcgggcgccg aaaaccagca gcacgaccac tattatgatt acccattccc agccgcctat
    14701 catggaggac acctttcgca acggggtgtt acaggcggta tttccaccag tagaacgcgg
    14761 acatgagtcc gagaatggtg agcagattga tattcaacgt gatgccgaaa gagatcgtca
    14821 ccacgatcaa attcaggacc agcactccgg aatcgtaggt gaagctgttg atcaggactc
    14881 tgtgcacgac gctggcggga cccacaaaaa ggctgagggc ggcgctcaga accgtcgcac
    14941 ccagaagccc tatgagcagg cttgcaaaca ggacaccagt actgcggtaa cgaggcatct
    15001 atctcctgct cccctccttg acccgttcca gggaaggctg tcccggagcg acggccgggt
    15061 gtcgaacaaa tgcatttccc agcgcacgaa ccagcccata caggacgtag tacaccccga
    15121 acgaaaagaa cgcctcggcg ggataccata ctgcgatgac caaaaaggcg atcatcacga
    15181 cgaggcgcac gcgttcagat gttggtccgt gccaggaaaa acgcggcatt ttgtcgtatc
    15241 tgatggtgct gaccatcagc agagccattc cgaacgcgag gaaggggagg acccagtcta
    15301 tgccgagatc aataatgaac tcgctgcggg agaacaccac aaaggacgct accataccgg
    15361 caccggcggg aatcggtaca cctttgaagg ccgctttgtc gaatcctttg agctgagcgt
    15421 tgaaccgtgc cagcctgagc gctccgcagg caagaaagac gaacgcaaca ggaaaagcga
    15481 tcccggaaaa acccgacgcg ttgagcagga tgaactggta gatcaataag gcaggcgcaa
    15541 aaccaaaaga gcacacatcc actatggaat ccagttctac accgaagtgg ctctgagttt
    15601 tgaatatgcg ggataccttg ccgtccagac cgtcgaaaag tcccgcggca atgatcaacc
    15661 acgcggcagt gacaaacgcc tcctccgttc cttccattgc tttgattgct gcaagcagcc
    15721 cacaaaacag acttccgaga gtcatgacgc tcggaagagc ctgcgtaaca agcggcatca
    15781 tggaagcact cctatcacag ttttgccgcc gacggcgcgc tggccgaccg cgacacggat
    15841 gctcgcatcc ggagggagaa tgacgtccac gcggcttccg aaacgaatca gtccgcaccg
    15901 gtctcccgcg cgcaccgtgt caccgacttt gagtcggcag acgattctgc gcgcgatgaa
    15961 gccggctatc tgtttgaagg caacggttcc ccgctcgcct ttgatggcga tgtgcgtttg
    16021 ctcgttatcc gccgatgctt tctcgttgaa cgccatcagg tatttcccgg ggcggtacac
    16081 catcgcggta acggaaccgg aaatcggatt tcggttcacg tgaacgtcca gaactgacag
    16141 gaaaatgctt acccggattg cattggtgcc cacataaggg tcgtcttctt gaacgatttc
    16201 cacgaccttg ccgtcagccg gcgacagcac cagcccttca ccggcgggag gtgttcggcg
    16261 cgggtcacga aaaaagaaca gcgaaaatgc tccaaaaacc agtgccagaa tgccgaggat
    16321 acggagcggc tcccacctga ggccgtgcca tgcggcgaga agcaatatcc ccgcacacac
    16381 gccgcccagg acgaagggaa gtccttcctt agccacggtc cctcctgttt ctcgggatct
    16441 cttccaggac aagtgtcacg gtgagttcac ggccgtttct gatgattcgg agttccacgc
    16501 gttgcccgac aacagcgcca aggaattcgt cccgcacatc ccggtcgctg gctatcgctt
    16561 tgccgttcac ggaaatgatc acatctccac gttgcaggcc tgcttttgct ccggggctgt
    16621 tgggttcgac attggtgatg attgctcctc gatctgattg gagcccgaga ctctcggcga
    16681 tccagcggtt cacgtcctga atgtgaatcc cggtccagaa gttgtggcgg acttcgccga
    16741 attgaacgat gtcgtccagc acctgcctcg ccttctgtat cgggatggca aagccgacgc
    16801 cttccgaacc tccgccctga gtgaagataa acgtattgat gccgataacg tacccttcgc
    16861 tgttcaccag cggcccgcca ctgtttcccg ggttgatgga cgcatcactc tggatcatgt
    16921 cccggtacgt acgtccgccg gccgaagagg gactgaaatc cctgttcagg gcgctgatca
    16981 cacccacggt aacggtggga tgagcgtcgc ggatcaggaa cccgaacggg ttgccaatag
    17041 cgatcgccca ttcgcctatc atgaggtcgt cgcttctccc caacggtgcg gtgggcagat
    17101 tctgagcgtc gatcttcagt acggcgatat cagcctgttc atccggttcc ccgaaaatct
    17161 tcgcgtcgaa ggagcggcca tccggcagat tcaccacgat ctgggtggcg ccccggatga
    17221 cgtgttcgtt cgtcagaatc atgccgtccg gggagactat gaaaccgcta cccatacttt
    17281 ggacgcggcg cacaaactcc tgccgaagtt ccgggaaaaa gaactgccac atcgggtcat
    17341 ccgcgaacgg aa
//
LOCUS       DAOTNG010000020        17158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3319_length_17158_cov_5.600070, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000020 DAOTNG010000000
VERSION     DAOTNG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17158
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3319_length_17158_cov_5.600070"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1620
                     /locus_tag="P5179_01915"
     CDS             256..1620
                     /locus_tag="P5179_01915"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRS94007.1"
                     /translation="MSFGQARATAMGGAYTAVATGVHAPAWNPANLAFFAAPSTELSL
                     GNLGFFAANDGFSYGDFVGWLDDEVLTTAEIQSALGMFPGGAISIRQTMNVGIPFSVR
                     VKQFAVTGEFMEFVSVGLPRGLFDAIADDRDATTAYNDARTSGIPYSLSGIVGDVWAV
                     GVTKFSYARRVPLRYADAFSVGATLNYYRASPRVRVVESVGEVLVRPNTWQTDARLVV
                     ETGGLSVKRTTDAFGDKQTDVEFEAANAWGLGCTLGAAGKLDDKTAISAALHNLPLRS
                     IMWNMAERRTYTLDSGSRIINAKSLIDDKPEGVNTLDYLDTLFAAPGSTVERYERINS
                     MKANVPAYLRVGMARSVDLFPAIWTVDFEQGFSRSALTSTTPRIAAGIEYLPVGPWIP
                     LRAGMSLGGRSGHFASLGMGFHLRWFHWDLALANEGAYTPFEVPFSKRARGLGFASEM
                     KLAF"
     gene            complement(1644..2888)
                     /locus_tag="P5179_01920"
     CDS             complement(1644..2888)
                     /locus_tag="P5179_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94008.1"
                     /translation="MGEGDSSSPEELVRRELDPGEVVLWSGAPSPETAFRSGLAYFVA
                     GSVVLFGFSALLTYFLLSATHDLALLVFLVVAGVPATCTAVWWMGTPFRRRHRAGNSI
                     CVLTDRRAIVFDQERSMRVQSFDAGLLAEAEATAGRDGRGDILFPAYVFLPSRKTGQF
                     NRRQAPAALARRGFRGIRDVEHVNALITQVSTRPAQGRAAPCEEVDLRAASLSRSGSD
                     AIRNAIATGEKVLWTGTPAASPVLIRWSPLMVLGGALAAVGIAVAFAPEIFAQSGVFL
                     SNYPLVVRVFGVGFAIAAFGFGLLPVREYRRLRRTVYAVTNRNAYILEAGPSSAVVRT
                     FSADDVRERYRRQQPDGSGDLVLQRRLRLEKAKRGAKGPGPEVQLAEEEVGFMGVQDV
                     RGAERCIRALEHGASQGTQAPR"
     gene            complement(2912..3577)
                     /locus_tag="P5179_01925"
     CDS             complement(2912..3577)
                     /locus_tag="P5179_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRS94009.1"
                     /translation="MDAPRNTREHVILELKRAGSLSTKDLAGLLGISSTAVRQILTVL
                     QAEGLIDTRTERQPQGRPCYVYTLTEKGHDLFPEAYDSLAIELLETVREIGGEEMIQD
                     LVAKQVSKKEERYRKLMKGRSLAEKLAQLRELRDGDGYMAVVEDGETKSAIREHNCPI
                     YRIAKNHPEMCAQEIALMERLLGVSIHQTHHMADGQHYCRFEIHKNQASATDPVKEAG
                     LAN"
     gene            3916..5328
                     /gene="glnA"
                     /locus_tag="P5179_01930"
     CDS             3916..5328
                     /gene="glnA"
                     /locus_tag="P5179_01930"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRS94010.1"
                     /translation="MTPGDVIKMAHDNGAEVVDLKFIDFPGMWQHFSIPVKFLEPDLF
                     EDGLGFDGSSIRGWQAINESDMLVVPDPNTAVMDPFTAVPTISMICDIHDPITKERYS
                     RNPRNVAKKAEAYLKSTGLADTAYIGPEAEFFIFDDIRYGQTINSGFYHIDSVEGNWN
                     TGRDEKPNLGYKPRLKEGYFPVPPVDHQMDIRTEMMLTMLKCGIEVEAQHHEVATAGQ
                     AEIDMKFCPLVEIADKLMMFKYIVKNVAAKNGKTATFMPKPLFGDNGSGMHVHVSLWK
                     EGKPLFGGNQYAGLSEMALYFIGGLIKHAPSLLAITSPTTNSYKRLVPGYEAPVNLAY
                     SSRNRSAACRIPMYSASPKSKRVEFRCPDPSCNPYLAFAGILMAGLDGILNKIHPGEP
                     LDKDLYDLPKEELAKVPKTPADLAAALDALEADHDYLLKGDVFTSDVIETWISYKRKN
                     EVDALRLRPHPHEFALYFDI"
     gene            5386..5724
                     /locus_tag="P5179_01935"
     CDS             5386..5724
                     /locus_tag="P5179_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRS94011.1"
                     /translation="MKKIEAFIKPYVLEEVKKALTDVGVKGMSISEVKGFGRQKGHTE
                     LYRGSEYHVDFLPKVKVEVVVSESMVEAAVDAIAKSACTGKIGDGKVFVLPVEKALRI
                     RTGESGEDIL"
     gene            5908..5981
                     /locus_tag="P5179_01940"
     tRNA            5908..5981
                     /locus_tag="P5179_01940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5942..5944,aa:Ile,seq:cat)
     gene            6002..6078
                     /locus_tag="P5179_01945"
     tRNA            6002..6078
                     /locus_tag="P5179_01945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6036..6038,aa:Val,seq:cac)
     gene            6297..7013
                     /locus_tag="P5179_01950"
     CDS             6297..7013
                     /locus_tag="P5179_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRS94012.1"
                     /translation="MKQGLFDLLGLQDVDNEIDALQKNKSAYPRRAAELRAEMEDLKQ
                     DRSAKENQLKQHESGFNHFLQQLNIAEENLKKHQTRLQEIKTNREYDAVQQEIIALEH
                     AIEEYFAERDREDEEVRRLRNLLDEERERFEARIAELESEIADLDEKAATIDSEIAKV
                     MEKRKAVAETVPPRILAVYERVRRGKTNAVVRVSRGACGGCWRSLSPQKLNELRMYNR
                     IIACDGCGRIVVWDDRESAG"
     gene            7111..8181
                     /locus_tag="P5179_01955"
     CDS             7111..8181
                     /locus_tag="P5179_01955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRS94013.1"
                     /translation="MNGVQMDNASEIVRGDSTQPACNEVLVPPSADGIRLDRAIAALL
                     PGISRSQASVAIRDGRVQLFSPKGKVVSHLKAGTPVAAGQTVRIVVARKDPLSVEPEG
                     IDLDIRYEDDDLLVVNKPAGLVVHPAPGHSGGTLVNALAGYSAELSRVGGTFRPGIVH
                     RLDKNTSGLLLVAKNDRAHAALAAQLKNRTISREYVALVWGHPSPRSGRIEAAIGRDR
                     AGGKTMSICGRSSRAATTRYEEISSFPYTSLVKLHLETGRTHQIRVHMKAIGHPVVGD
                     PEYGGREAAIRGIAPAHRSSARFLLTVIGRQALHARRLSFLHPATGKALVVESDPPED
                     FRRALSIVAGAVPQAADLSYFR"
     gene            8343..10601
                     /locus_tag="P5179_01960"
     CDS             8343..10601
                     /locus_tag="P5179_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94014.1"
                     /translation="MLTLRNLWCRRGFSAICAIGLCASSFSAVFAQDDREGEPATPYR
                     DAYIAYVDRQLSEIQSQIAFQRSILNERGGELLDEIGIRLQEKLALLPPEMRQPGIPE
                     PPEIVALREYEQTLSNEVVQIEDRILSSADMISEQRDSVAGELKSADAVRATVTELDR
                     SITTDLELWKTKVDLVRSRLIGSGSADEGRRMFVTEYNALVSHYDAGDYRSARARLTA
                     LATAYGDIPLPEWRERLLFYRAEIDYAMGDWDNAFRGYMEVWQRPRNEFASVALLNWT
                     ELAFAAGEHRKIATMWTAQPRLPEDVVDANRIRVLVAESFLRLNEPQVALSVLNLVQT
                     ALPPAAEDAVDDEETKLANDRLVVYSRLLRAEAHLGGDSGVATLQGAASEETLPEVTD
                     SALDSMLAATGGTPQRGDPNYYSRMAYLIASGQVQAERPGREAGTRVAAAVKELEDVV
                     PLVGPIDPEGTLMARVSMSLGHAYFQANRMEDAARAYGAVPNTSVWYPEAQLGRAWAF
                     IETGNYLDAKIAVNTAQRWPLSPPAALEAAALNSYVLSLIGETELAAEQMREMKSMVD
                     IQQRRAARVWVNQQLQKIDGSLHLVGIIALERQNEALYRSAIEEQASLLALREQVQRV
                     EQYLQSYTPNATGLQDSLTVAIRAEQEALRGLEQKVANLRRAAGQTPAAAANLPEITY
                     EQRREQLQRWLQGVQPQMTPQGSQVYGKWVQYAEFAYSRRVFDENQRRRYEADRLKDV
                     RTRIQKLLSESP"
     gene            10637..12796
                     /locus_tag="P5179_01965"
     CDS             10637..12796
                     /locus_tag="P5179_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94015.1"
                     /translation="MRLSRSIAVWRGICGVRAQISSAVCVVALAGLCASSVKAQSASP
                     TDTSSLPALLQSSLDGAMTVESVLLGDRRALPPIPRRQFSVGTIPLPPVSPGDTIIVP
                     QVTGEITDPELKERANSLNRSLRLLAIEERLLGSELWGGAPRTGVIPRKDFLEARINL
                     LRDMENVLQIRLREKLAQLDTTIARVESEIEANRTAEIASLEDYLDKYPSSASVADAK
                     FILGQLYYDKERKRYTDAMLRYTTEFQRYRMGLLPVAPAPPVLNEAVSVPFYKSVVEL
                     GTNPDLVPYSLYSLGKFHLEQARDLKARSDESRLTGRREEAQHFTHLSEMETDSAKTY
                     FARLVVEFPDDSVNVPEAFYVLASHYNMLGGPVNRDTAAVYCNALIRNFWYSPRYQNA
                     LVMLGQINYYNGVGTRERAARNRYFSNALAYMAWLARDVDAFTESQIPGVSPDPPRMM
                     DITRRDYAVQFMTQIITRQSPLPGTEPPPPVETAVKLVTAAGNPPFGADLLRQVGDKK
                     NSDYNTSGNPVDLVAALTAYDSLLSRYPTYKEGPQIQQVVIDGATYLSDDPAERYRIY
                     VRQKLLFFERFNRNSEWARQNQDSPAVVKAADDSAAAYLETGARWLYSTARAANDREG
                     VRRSLDYFVKFFQTYPERPQAYELNWSLATELRDLGDYERAYEEFMRVSNADRSQYRE
                     DAALEAVAAAQALVEMEQSGSRSPGRAQETQPPGGGR"
     gene            12799..16836
                     /locus_tag="P5179_01970"
     CDS             12799..16836
                     /locus_tag="P5179_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94016.1"
                     /translation="MMTFSRLPKFRVLRSLVGAGLVSLLIGFLVIGGKPSPAPAQFSA
                     EELERNPNLTPSVRRLILAYENYLKHFPDSPQGKEFVIDIAERFRSAGDNETAIVAQK
                     RVLARPDITQAERAYAYEQIMECYRLLGKFDEQEEWARRMAAADVGREKQQQAKDFIF
                     AAGYNRAKALEDSSRLMDAAEAYERLAVMNPDHGQASEAMLKAADLYDTAGDKARAAR
                     TFERFYYTYPDYRDPASGKGALVALENAANLYAEINDYRHTADAAERILAAAPDHPNR
                     QMYMNNLAAIYSLLKDYNNAIRVRQQYVALYPNDPKSGTYQWDIAQFRGLAGQRQQQL
                     AEYEAFIRSYPNDFRAIEANLIVGQDKLAQRDRALARGARDEAERLQALARANFEKAF
                     TLHDSLEALRQGSGDLLHALPAAVEVAKMDSAAYYSISLRGSPSFSQDSTRKWESLRQ
                     AEAMYRKIANYAYPPTTFAALYQRGRLFEDFSAEYLKQPRPDSAVTYDEINKVFFINE
                     VSKQFLQVLAIPAYQTQMLEFYETNRASIDTAAATLPDTSLRTAHLPWLDMARERLAA
                     IPRTLDSLTLNTIAYDADLTVLKAQEKIPQTIAEKWPAFERQQQERYRTDPRLRFGDK
                     QEIFDRGVAPVVYGSGPQDSLALVNYFQRIIADGERLGASRDWVLYNKGRLRLVYGAR
                     SSYFSTVTREGMENLGFQLDTLRQESNGLTNIIARLPKLDLAAIGPPPERPDLTPPAQ
                     PVRPNVDLRAPENRAIALRFIEDFNRFKRQVEDLKYRAVRYQREVERYRARVAELQAS
                     TAAQFRGILEEARTQSQEFSRVATETQRYRMAIELVADRTRNAYERDIAFGDSVGYAE
                     KDRRAVRDSALAANHRISLTLDSLYSEAAATRRYWEERRDSTEGGEASPAFQVINNLV
                     TQYAAVADSFRVTAVRSYMYTYKGRDSIFTVGLDHPIVQYAIQRLRVIDPTFGVQTVP
                     LTFTYVTEDSTGLWRVSSTIDRQNPDAWRQVAFNDSTWVPAVKGTMPRVEFVQAADTT
                     AGAAGQPEPAAGEGAVTAEGDTTAEAAPQVAEVPKIVGFPPDNLNMTDLWSPTYADTV
                     YFRYKLQLPPRWDELPDSLSEPGRKRPVVRKASITITADDDYVVYVNGEVTPARDTDA
                     RVDWKTARTYEVLKDQWTVGDTANIIAIRARNEKRLNRIPDTDTTTYGVMARIDIEMD
                     VPLYIYEALYRPPEPEPTFVLAFTPDDSVKMADTTGTYFRTAAERERWQECRTRTLRA
                     AWEDTVLIPWRAQRDALKKAQLDSEIVRLTRWIRQRQEEARGRIATAAAREAGLEESG
                     ESPAPDAGTDSPQETPARPEDTGGP"
     gene            16849..>17158
                     /locus_tag="P5179_01975"
     CDS             16849..>17158
                     /locus_tag="P5179_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94017.1"
                     /translation="MRNSRLVALSISALCAVVLLAPLAGAQEAVRTVRAAGTIREKPP
                     GELATVVRRDPDFRTIKYLGMQRDYRDELRSKLMLGHAVIRHGRPVLPMEPIVWPPEP
                     A"
BASE COUNT         3686 a         4940 c         5183 g         3349 t
ORIGIN      
        1 tcggctcgac gaccagttgc agttgttttc ctacgaaaac ctttatctaa tgccgcgtat
       61 ccagcttctc gatgcaccgg gtctcgttca gttgtcacct tcggattatg tgaaggttgc
      121 aatctggatg caggtcgccg gcgcgaccaa gaggtaacgc ccatggattg caggaaaggc
      181 gcgtatttgt gtgctggctt tttgctgacg gcactgtcga tcggggtgct gccacggtgc
      241 tccagggcgg ccaccatgtc tttcgggcaa gcccgcgcaa ccgccatggg gggcgcgtac
      301 acggcagttg ccacgggcgt tcatgcgccc gcgtggaatc cggccaacct cgcattcttc
      361 gccgcgccgt caaccgaact cagcctgggg aacctgggct tcttcgcggc gaatgacggt
      421 ttctcgtacg gtgattttgt cggttggctg gatgacgaag tgctcaccac ggcggaaatc
      481 cagtccgccc tcgggatgtt tccgggcggt gccatcagca tccggcagac gatgaacgtg
      541 gggatacctt tttcagtgag ggtgaagcag tttgccgtca ccggcgagtt catggaattc
      601 gtgtccgtcg gtctgcctcg agggcttttt gacgcgattg ctgatgaccg cgacgccacg
      661 actgcgtaca acgacgcccg tacttccggc attccgtatt cgctgtcggg gatagtcggt
      721 gacgtgtggg ccgtcggtgt aacaaagttt tcctacgcgc gccgtgtgcc gctgcgttac
      781 gccgacgcgt tctccgtcgg cgcgacgctg aactactacc gtgcgtcgcc ccgggtccgc
      841 gtggtggaat cagttggcga ggtgcttgtc cgtccgaaca catggcagac ggatgcgcgg
      901 ctggtggtgg aaacaggagg gctttctgta aaacggacca cggacgcgtt cggcgacaag
      961 caaacggacg ttgagttcga agcggcaaac gcctgggggc tcggttgtac gcttggcgct
     1021 gcggggaaac tggacgacaa aaccgcaatc agcgcggcgt tgcataatct tccgctgcgt
     1081 tccatcatgt ggaacatggc ggagcggcga acctacacgc tggatagcgg atcgcggatc
     1141 atcaatgcaa aatcgctcat cgacgacaaa cccgaagggg taaacacgct cgattacctc
     1201 gatacgctgt ttgccgcgcc gggaagcacc gtcgaacggt acgaacggat caattccatg
     1261 aaagcgaacg taccggctta tctgcgcgtg ggtatggcgc ggtcggtgga cctgttcccc
     1321 gcgatctgga cggttgattt cgagcagggc ttctccagaa gcgccctgac cagcaccact
     1381 ccgcggatag ccgccggaat agaatacttg cccgtcggtc cgtggatccc gttgcgcgca
     1441 ggaatgtcac tgggaggcag atcgggccat ttcgcgtccc ttggaatggg cttccatctg
     1501 cgatggttcc actgggatct cgcgcttgcc aacgaaggcg catacacccc gtttgaggta
     1561 cccttctcca aacgggcgcg gggactcggt ttcgcgagcg aaatgaaact ggccttctga
     1621 tacggggcgc tgccagggca ccctcagcgt ggagcctggg tgccctgaga tgctccgtgt
     1681 tccagcgcgc ggatacaacg ctccgcaccg cgcacatctt gtacacccat gaaaccgacc
     1741 tcttcctcgg cgagctgaac ctcgggaccc ggccctttgg cgccgcgttt cgctttctcc
     1801 agacgcaggc gtctctgcaa cacgagatct ccggaaccat ccggttgctg gcgtcggtag
     1861 cgctcacgaa cgtcgtccgc ggaaaacgtg cgcacaaccg cggacgaggg gcctgcctcg
     1921 aggatgtagg catttcggtt ggtcaccgcg tacaccgttc ttcgcaatct cctgtattcc
     1981 cgaacaggaa ggagaccgaa gccgaacgca gcgatggcaa aaccgacgcc gaagacccgc
     2041 acgaccaggg ggtaattcga aaggaatacg ccagactgcg cgaagatttc cggggcgaag
     2101 gccacggcaa tccccactgc ggcaagcgct cccccgagca ccatcaaggg agaccatctg
     2161 atgagaaccg gcgacgcagc aggggtcccg gtccacaaca ccttctcgcc ggtggcgatg
     2221 gcgtttcgaa tcgcgtcgct tcccgaacgg gaaagggagg cggctcgcag gtcaacttcc
     2281 tcgcacggcg ccgctctgcc ctgcgccggt cgtgttgaga cctgcgtgat aagcgcgttg
     2341 acatgctcca catccctgat tccgcgaaat ccccggcgcg cgagagctgc gggcgcttgc
     2401 ctgcggttga actgcccggt tttcctcgac ggcaggaaca catacgccgg gaacaaaatg
     2461 tcgccacgcc catccctgcc agcagtcgcc tccgcctccg ccagaagacc ggcgtcgaat
     2521 gactggacgc gcatgcttcg ctcctggtca aacacgattg cccgcctgtc cgtgagaaca
     2581 cagattgagt tccccgccct gtgccgtcgc cgaaacgggg tacccatcca ccacacggcc
     2641 gtgcaggtcg cgggtacgcc tgccacgacg agaaacacca gcagcgcaag atcgtgggtc
     2701 gcagacagga ggaaatacgt caggagggca gaaaaaccga acaggacgac cgaaccggcg
     2761 acgaaatagg caaggcccga ccggaacgcg gtctccggag aaggagcccc cgaccagaga
     2821 acgacctcgc ccggatcaag ctcacggcga acgagctcct caggcgaact gctatccccc
     2881 tcccccacgg gtgcttcccc ttttccgaat ctcagttcgc aagtccggcc tccttgacgg
     2941 ggtcggttgc agatgcctgg tttttgtgaa tctcgaatcg gcagtagtgc tggccgtccg
     3001 ccatgtggtg cgtttgatga atcgaaaccc cgagcaaccg ttccatcaag gcaatttcct
     3061 gcgcgcacat ctcaggatgg ttcttcgcga tgcggtatat tgggcagttg tgttcacgga
     3121 tcgcagattt tgtttctcca tcctcgacaa ctgccatgta accatctcca tcgcgcagct
     3181 ctcgcagttg cgccagcttt tcagcaagcg agcgcccttt cataagtttc cgatagcgct
     3241 cctctttttt gctgacctgc tttgcgacga gatcctgaat catttcctcc ccgccgattt
     3301 ctcgcacggt ttccaacagc tctatcgcca gcgaatcgta cgcctcaggg aaaagatcat
     3361 gacccttttc ggtcaacgtg tacacgtaac aagggcgccc ctggggctgc cgttcagtcc
     3421 ttgtgtcgat caggccttcc gcttgcagca cagtgagaat ctgtctgacg gcagtactgg
     3481 aaataccgag aagtccggcc aggtctttcg tactcaggct tcccgccctc tttagttcca
     3541 gtatgacgtg ctctcgggta ttcctcggtg catccatgcg gtttccttgc ggctttggaa
     3601 tgggaaagct ctgaagacaa tattctactt gcgaaaatgt gctgagtcca gttccgctaa
     3661 agggattcac ccggaggaaa ccggcgcacg gcactcaaac acctataccg ttcttggccg
     3721 ccgaaaattc cgttgtccgc gtttacgccg atttaacaca cgcgcaaaaa cggcgaaaca
     3781 cataacacgt atccttttat aacgagatcg caggggcggg ttcgcctcat ggttctgccg
     3841 gtggtggcca aatggcgcga aagctgccgg ggctgagatg acacagttca taaaaaggag
     3901 acaaaaccga tgggaatgac gcccggtgac gtgatcaaaa tggcgcatga caacggtgcg
     3961 gaagtagttg atctgaagtt catcgatttc cccggaatgt ggcagcactt cagtataccc
     4021 gtcaagttcc ttgagcctga ccttttcgag gacgggttgg gctttgatgg ctcgtcaatc
     4081 cgcggatggc aggcgatcaa cgagagcgat atgctcgtgg tgcctgatcc gaataccgcg
     4141 gtcatggacc ccttcactgc cgttcccacc ataagcatga tctgcgacat tcacgacccg
     4201 atcacaaagg aacgttactc gcgcaatccg cggaatgttg cgaaaaaggc ggaagcgtac
     4261 ctgaagtcaa cagggctggc cgacaccgcc tacatcggcc cggaggcgga gtttttcatc
     4321 ttcgatgaca ttcggtacgg ccagaccatc aattccggtt tctatcacat agactccgtc
     4381 gagggtaact ggaacaccgg gcgtgacgag aaacccaact tggggtacaa accgcgcctt
     4441 aaggaagggt acttccccgt gcctcctgtt gatcaccaga tggatatccg cacggaaatg
     4501 atgttgacaa tgctgaaatg cggcatcgag gtggaagcgc agcaccacga agttgcaaca
     4561 gcgggacagg cggaaatcga catgaaattc tgcccgctgg tggagattgc agacaagctg
     4621 atgatgttca aatacatcgt gaagaacgtt gcggcgaaga atgggaagac cgccacgttc
     4681 atgccaaaac ccttgtttgg ggataacggg tcaggcatgc atgtgcatgt cagcctctgg
     4741 aaagagggga agccgctctt cggtgggaat cagtacgcgg gcctgagtga aatggcgctc
     4801 tacttcatcg ggggccttat caagcacgcg ccttcgcttc tggcaatcac cagcccgacg
     4861 acgaactcgt acaagcggct cgttccgggc tacgaagcgc cggtgaatct tgcgtactct
     4921 tcgcggaatc gctccgctgc gtgccgtata ccgatgtatt cggcgtcgcc gaagtccaag
     4981 cgtgtggaat tcaggtgtcc cgatccgtcc tgcaacccct atctcgcgtt tgccgggata
     5041 ctcatggcgg ggctcgatgg cattctgaac aaaatccacc cgggagaacc gctcgacaaa
     5101 gacctgtacg atctcccgaa agaagaactg gccaaggtcc cgaaaacgcc cgcggacctc
     5161 gcggcggcgc ttgatgcgct cgaagccgat cacgactacc tgctcaaagg ggacgtgttc
     5221 acttccgatg tcatcgaaac ctggatctca tacaagcgga aaaatgaggt cgacgcgctg
     5281 cgccttcggc cccatcccca tgagtttgcg ttgtacttcg acatctgata cagacgggtg
     5341 tttgcggcgg cgggccgttc ctggccttga gaaaggacga ttcccatgaa gaagatcgag
     5401 gcgttcatca aaccgtacgt tctcgaagaa gtaaaaaagg ctctcacgga cgtcggtgtg
     5461 aaagggatgt cgatctccga agtgaaaggc tttggcaggc agaaagggca cacagagctt
     5521 taccgcggga gtgagtacca cgtcgatttt ctgcccaaag taaaggtgga agttgtcgtg
     5581 tccgaatcaa tggtggaagc ggcagtggat gcgatagcga aatcagcatg cactggcaag
     5641 atcggcgatg gaaaggtgtt cgtgctgccg gtcgagaaag ccttgcggat ccggacaggg
     5701 gaaagcgggg aagacattct ctgagagtca ccaatgaaga acatgagccg gggagggact
     5761 ctgcgccctc tccggcttct tgttgagagc cttcccgcgg taaccgacca ccggacctgc
     5821 tgagggaacc cgaactccgc gcggcggtcg tacccttgcc tgcctgcggt tgcctcgtat
     5881 cttttattca tacgtcaagg atttttcgcc cccatagctc agttggttag agcccccggc
     5941 tcataaccgg tctgtcccag gttcaagtcc tggtgggggc atcagtaaac atttgttttt
     6001 tgggcggtta gctcagctgg ttagagcgct ggtctcacat accagaggtc acaggttcaa
     6061 ttcctgtact gcccaccaac tctttgtgga tgatactcgg tgaggcggac tcccggaagc
     6121 agttgtcgcc cgttgcggct tggaaaaagc gagccgaaac gggcgacacc ggcgatgtat
     6181 gatccggaac cgaggggacg cggaaaacgg ggtggtattt tcgataccac cccgttttct
     6241 gtttgatccg ccgcctcgcg caacgtggcc ttggcaatgg aggatggaaa acgaagatga
     6301 aacaaggatt gttcgacctc ctgggattgc aggacgtaga caacgagatc gatgcacttc
     6361 agaagaacaa gagcgcgtat cctcgacgtg ctgccgaatt gcgcgccgag atggaggatt
     6421 tgaaacagga tcggtccgcc aaggaaaatc agctcaagca gcacgaatcg gggttcaacc
     6481 atttccttca gcagttgaac atcgcggaag aaaatctcaa gaagcaccag acgcgcctgc
     6541 aggaaataaa aaccaatcgc gaatacgacg cggttcagca ggaaatcatc gcgcttgagc
     6601 acgcgattga ggaatacttc gcggagcgcg atagagaaga tgaggaagtc cgcaggttga
     6661 gaaacctcct ggacgaagag cgcgagcgct tcgaagcgag gattgcggaa ctggagagcg
     6721 aaattgccga ccttgatgag aaagcggcta ccattgatag cgaaattgcc aaggtcatgg
     6781 aaaagcggaa ggcagtcgcc gaaacagtcc ctccccggat actggcggtc tacgaaagag
     6841 ttcggcgtgg caaaaccaac gcggtggtaa gggtttcgcg gggtgcctgc ggcgggtgtt
     6901 ggcggagcct ctctcctcaa aaactgaacg aattgcgcat gtacaaccga attattgcct
     6961 gtgacggctg tggccgtatc gtcgtgtggg atgacaggga gtccgccgga tagttctgga
     7021 tgactccgcg cgaaaaacga tgagatctca gagcgccaac gtgagcagcg gtgctgttta
     7081 cgccggcgct ttctatcggt gggcgtggcc atgaacggcg ttcagatgga caatgccagc
     7141 gagattgtgc gcggtgattc gacgcaaccg gcgtgcaacg aagtcctggt tcctccctct
     7201 gcagacggta ttcggctcga tcgcgccatc gcggctctcc tccccgggat cagtcgttcg
     7261 caagcgtcgg tggcgatccg cgatggacgg gtgcagctct tttctccgaa agggaaggtc
     7321 gtctcgcatc tcaaagccgg cactcctgtt gcggcaggcc agaccgtgcg cattgtggtt
     7381 gcccggaaag atccgttgtc cgtagagcct gagggtattg acctggacat caggtacgaa
     7441 gacgacgacc ttctggttgt gaacaaacct gcgggattgg tagtccaccc ggcccccggc
     7501 cattccggcg gaaccctcgt gaatgcgctg gcgggttaca gcgccgaact ttcccgggtg
     7561 ggcggaactt tcaggccggg gattgtacac aggctggaca aaaacacctc cggccttctt
     7621 cttgtggcga agaacgatcg cgcccacgcg gctctcgcgg cccagctcaa aaatcgcacg
     7681 atttcgcgcg agtacgtcgc ccttgtgtgg ggacatccgt cgccccgttc cgggcgcata
     7741 gaggctgcca tcggacgcga ccgggcgggt ggaaaaacca tgtcgatctg cggcagatcg
     7801 agccgcgcgg ccacaacgcg atacgaggaa atttcttctt ttccgtacac gagtctggtg
     7861 aaactccacc tggaaacagg aaggactcat cagatccgcg ttcacatgaa ggcgatcgga
     7921 caccccgtag taggggatcc ggaatacggc ggaagggaag ctgcaattcg agggatcgcc
     7981 cccgctcaca gatcttctgc gcggttcctt ctcactgtga tcgggaggca ggcgcttcac
     8041 gcgaggagac tgtcttttct gcatcctgcc acaggtaagg cgctcgtggt ggaatccgat
     8101 cctccggagg atttccggcg cgcgttgagt attgttgcag gcgctgttcc acaagccgcg
     8161 gatttatctt attttcgata gggagtaccg aatcgaggag cgaatactga caccccgcgc
     8221 aacccgtcag ttgacaagag attgtcgttc aagtaatttg tgtgcatgcc tacgcgcaaa
     8281 atcttggtag gtttgctgct tcgccacgat agcgcggttc ctaccgcgga aagacgacgg
     8341 gaatgctgac actgaggaat ttgtggtgcc gcaggggctt ctcggcgatc tgtgcgattg
     8401 gcctttgtgc ctcctcattt tctgcagtgt tcgcgcagga tgaccgcgaa ggcgaaccgg
     8461 caacgcccta ccgagatgcc tacatcgcgt acgtcgaccg gcaactctcc gaaattcagt
     8521 cccagatcgc cttccaacgg agcattctca acgagcgggg cggcgaactc ctcgatgaaa
     8581 ttggaattcg ccttcaggag aaactcgcgc tccttcctcc ggaaatgcgc caaccgggga
     8641 tcccggaacc tccagaaatc gttgctttga gggaatatga acaaactctc agcaacgagg
     8701 tggtccagat tgaggacagg atcctgtcct cggccgacat gatctcagag caacgcgatt
     8761 ctgttgccgg tgaactgaaa tcggcagatg ccgtgcgcgc cacggtgaca gaactggatc
     8821 ggtccataac gacagatctc gaactctgga aaacgaaggt cgatcttgtg agatcgcgcc
     8881 tgatcggttc aggcagcgcc gatgaaggcc ggcgcatgtt cgtcaccgaa tacaacgcgc
     8941 tggtatccca ttacgatgcc ggtgactatc gctccgcgcg cgctcgattg accgccctgg
     9001 ccacggcata tggcgacatt ccgcttcctg agtggcgcga gaggctgctg ttctaccgtg
     9061 cggaaatcga ttacgcgatg ggcgactggg ataacgcctt caggggctac atggaggtgt
     9121 ggcaacggcc gcggaatgag ttcgcttcgg ttgccctcct gaactggacg gagctggcgt
     9181 ttgcagcggg cgaacaccgc aagatcgcaa ccatgtggac cgcacaacct cggctgcccg
     9241 aagacgtggt ggatgccaac cgcattcgcg tgttggttgc ggaaagcttc ctgcgcctca
     9301 acgagccgca ggttgccctt tcggtgttga atcttgtgca gacggcattg ccacccgcgg
     9361 cagaggatgc cgtggacgac gaggaaacaa aactcgcgaa cgatcgcctg gtagtctatt
     9421 cacggctttt gagagccgaa gcgcacttgg gcggcgatag cggcgtggcg acactgcaag
     9481 gtgctgccag cgaggaaacg cttcccgaag tcacggattc ggctctggat tcgatgctgg
     9541 cggcgacggg aggcactccc cagagggggg acccgaacta ctattcacgc atggcgtacc
     9601 tgatcgccag cgggcaggta caggcagagc gccccgggcg cgaagccggg actcgcgtcg
     9661 cggccgccgt gaaagaactt gaagacgtcg ttcccctcgt cggtccaatc gatcctgagg
     9721 gaacgttgat ggctcgtgtt tccatgtccc ttggccacgc gtattttcag gccaaccgca
     9781 tggaggacgc tgccagggca tacggggcgg ttccgaatac gtctgtgtgg tacccggaag
     9841 cgcagctggg gcgcgcgtgg gcgtttatcg agacaggaaa ctatcttgac gcgaaaatcg
     9901 cagtgaacac agcccagcgt tggccactga gcccccctgc ggctctggag gccgccgctc
     9961 tgaacagcta tgtgttatcg ctgatcggag aaaccgagct tgccgctgag cagatgcgcg
    10021 agatgaagtc catggtggac atccagcagc gtcgcgccgc gcgtgtatgg gtgaatcagc
    10081 agctccagaa aattgatggc tctctgcacc ttgtcggcat catcgcgctg gaacgccaga
    10141 atgaagcact ctaccgttcc gcgatagagg aacaggcgag tctcctggca ctgcgcgaac
    10201 aggtccaacg ggtggagcaa tacctccagt cctatacgcc gaacgcgacc ggtcttcaag
    10261 acagcctcac cgtcgcgatc cgcgcagaac aagaggcatt gcgagggctt gagcagaagg
    10321 ttgcgaacct gagacgtgcg gcgggtcaga ctcccgcggc cgctgcgaat ttgcctgaga
    10381 ttacgtacga acagcgtaga gagcagctcc agcgttggct gcagggtgtc caaccccaga
    10441 tgactcctca gggatcccag gtgtacggca agtgggtgca atatgcggaa ttcgcgtatt
    10501 ctcggcgggt ttttgatgag aaccaacggc gccgttacga agcggacaga ctgaaggacg
    10561 ttcgtacccg catccaaaaa ctgctttcgg agtcgccatg acccaccgct ttcgatgtgc
    10621 ctttcaggcg cccctcgtgc gactgtcgcg ttcgattgcg gtctggcggg gaatctgtgg
    10681 tgttcgcgca cagatttcca gcgcagtgtg cgtggtcgcg cttgcaggcc tctgcgcgtc
    10741 gagcgtgaag gcgcagagcg cttctccgac ggacacgagt tctctgccag cgctcttgca
    10801 gtccagcctc gacggagcga tgacggtgga atctgtcctg ctgggtgacc gccgggctct
    10861 tccgccaatt ccgcgccggc aattcagtgt gggtaccatt cccctgcccc cggtttcgcc
    10921 cggcgatacg atcatcgttc cgcaggtaac gggtgagatt acggatcctg aactgaagga
    10981 gcgggcaaac tccctgaaca ggagtctgcg gctgcttgcg atcgaagaac gcctgcttgg
    11041 gtcggaattg tggggtggcg cgccgcgaac gggcgtgatt cctcgcaaag acttccttga
    11101 ggcgaggatc aatctcctgc gtgacatgga gaacgttctc cagatacggc tccgggaaaa
    11161 actggcgcag cttgatacca ccattgcccg ggtcgaatcc gaaattgagg cgaacaggac
    11221 agcggaaatt gcgtcgcttg aggattacct cgacaagtac ccgagctccg cgtccgttgc
    11281 agacgccaag ttcatactcg gccagctcta ttacgacaag gaacgaaagc gctacacgga
    11341 tgcgatgctg cggtacacga ccgaattcca gaggtaccgc atggggcttc tgcccgtcgc
    11401 accggcgcct ccggttctga acgaagcagt atccgtcccc ttttacaaat cggtggtgga
    11461 actcggtacg aacccggatt tggtgccgta ctcactctac tcattgggca agtttcatct
    11521 tgagcaggcg cgtgatctga aggcgcggtc cgatgaaagc cggttgacgg gacgccgtga
    11581 agaagcccag catttcacac acctgagcga aatggaaacg gattccgcca aaacctattt
    11641 cgcgcgcctc gtggtggagt ttcccgacga ttcggtcaat gttccggaag cgttctacgt
    11701 cctggcctcg cactacaata tgctcggcgg accggtgaac cgcgataccg ccgcggtgta
    11761 ctgcaacgcg ttgatcagga atttctggta ctcaccgcgg tatcagaacg cgctcgtaat
    11821 gctggggcag atcaactact acaacggtgt cggaaccagg gaacgcgccg cgcggaatcg
    11881 ttacttttcg aacgcacttg cctacatggc ctggttggca cgcgatgttg acgcgttcac
    11941 agagtcccag atacccggtg tgtctccgga tccaccgcgg atgatggata ttacgagacg
    12001 cgattatgcc gtgcagttca tgacgcagat tatcacgcgc caaagtccgc ttcccggaac
    12061 ggaacctcct cctcctgttg aaacggcggt gaaactggtt actgcggcag gcaatcctcc
    12121 gttcggagcg gacctgttga gacaggtcgg agacaagaag aacagcgact acaacaccag
    12181 tggcaatccc gtggacctcg ttgctgcgct caccgcgtac gacagcctgt tgtcccgcta
    12241 tcctacatac aaagaaggtc cgcagattca gcaggtggta atcgacggtg cgacgtacct
    12301 ctcggacgat cccgcggaac ggtaccgcat ctacgttcgg cagaaactgc tgttcttcga
    12361 acgattcaat cgaaacagcg aatgggcgcg acaaaatcag gactcgcctg cggtggtcaa
    12421 agcggcggac gattccgccg ccgcgtatct tgaaacaggc gcgcgatggt tatacagcac
    12481 cgcacgcgcc gcgaatgacc gcgagggcgt gcggaggagt ctggattact tcgtgaagtt
    12541 cttccagacg tatccggaac gtccacaggc gtacgaactg aactggagcc ttgcgacaga
    12601 attgcgtgat ctcggcgact atgagcgcgc gtacgaggaa ttcatgcggg tttcgaatgc
    12661 cgatcggagc cagtaccgcg aggatgctgc gttggaagcc gtggctgcgg cgcaagcgtt
    12721 ggtcgagatg gaacagtccg gttcccggtc acccggacgg gctcaggaaa ctcaaccgcc
    12781 cggtggagga cgctaggcat gatgacgttc tcacgccttc cgaaattccg cgtcctccgt
    12841 tcactcgtcg gtgcgggtct ggtgagcctt ctcatcggct ttctggtgat cggaggtaaa
    12901 ccctctcccg ctcccgccca gttttcggct gaggagctgg aacggaatcc gaatcttacg
    12961 ccgtcagtcc gcaggctcat tctcgcctac gaaaactacc tcaagcattt tccggacagc
    13021 ccgcagggca aggaatttgt gatcgacatc gccgaacgtt tccgctcggc gggcgataat
    13081 gagacggcga tagtcgcgca gaaacgcgtg ctggcccgtc ctgatatcac gcaggcggaa
    13141 cgcgcgtacg cgtatgaaca gataatggaa tgttaccgcc tgctcggaaa gtttgacgaa
    13201 caggaggaat gggcgcgccg gatggcggca gccgatgtcg gccgcgagaa acaacagcag
    13261 gcgaaggact tcatcttcgc ggccggatac aaccgggcaa aggcactcga ggacagctca
    13321 cggcttatgg atgccgccga agcgtatgaa cgtctggcgg tcatgaatcc cgaccacggg
    13381 caggcgtcgg aagccatgct caaagccgcg gacctgtacg acaccgcagg tgacaaggca
    13441 cgtgcggcga ggacgttcga acgattctac tatacgtacc ccgattaccg cgatcctgcc
    13501 agtggaaagg gtgcgcttgt cgctctcgag aacgcggcca acctgtacgc ggagatcaac
    13561 gattaccgcc acacggccga tgccgcggaa cggattcttg ccgcagcgcc ggaccatccg
    13621 aaccgccaga tgtacatgaa caacctggcg gcgatctaca gcctcctgaa ggactacaac
    13681 aacgcgattc gtgtgcggca gcagtacgtt gcgttgtacc ccaacgatcc caaatcaggg
    13741 acataccagt gggatatcgc tcaattccgg ggccttgcgg gccagcggca gcagcagctc
    13801 gccgaatacg aggcatttat ccggagctat cctaacgatt tccgcgccat cgaagcgaat
    13861 ttgatcgtcg gacaggacaa gcttgcgcag cgtgatcgag ccctggcacg aggcgccaga
    13921 gacgaggcgg aacggttgca ggcgctggca cgggccaact tcgagaaagc gttcacactg
    13981 catgattccc tcgaagcctt gaggcaaggt tcgggggatc ttctgcacgc gcttcccgcc
    14041 gcagtcgaag tggcgaaaat ggacagcgcc gcgtattact cgatctcatt gcgcggttca
    14101 cccagcttct cgcaggatag cacaagaaaa tgggagtcgt tgcggcaagc cgaggcgatg
    14161 taccgcaaga tcgccaacta cgcctatccg ccgacgacgt ttgctgcgtt gtatcaacgt
    14221 ggtcgcctgt ttgaggactt ctctgccgag tacctgaaac aaccgcgtcc cgattcggcc
    14281 gtcacgtacg acgaaatcaa caaggtgttt ttcataaacg aggtatcaaa gcagttcctg
    14341 caggtcctcg caattccagc gtatcagact cagatgctgg aattctacga gacgaaccgt
    14401 gcctcgatag acaccgcggc ggcgacgctc ccggatacat cactcagaac ggcgcatttg
    14461 ccgtggcttg acatggcgcg cgagcgactt gccgccatac cgagaacgtt ggacagcctt
    14521 accctgaata cgatcgcgta cgacgctgac ctgacggtgc ttaaggcgca ggagaaaatt
    14581 ccacaaacca tcgccgagaa atggccggcg ttcgagcggc aacaacagga acgctatcgc
    14641 acggacccgc gcctgcgttt cggagacaaa caggagattt tcgaccgcgg tgtggcaccc
    14701 gttgtctacg ggtcggggcc ccaggattct ctggcgctcg tgaactactt ccagcggatt
    14761 attgcggatg gcgaacgcct tggcgcctca cgggactggg tgctgtacaa caagggccgt
    14821 ctccgcctcg tgtacggagc tcgaagctcg tacttctcga cggttacgcg ggagggcatg
    14881 gaaaacctgg gattccagct tgatacgttg cggcaggaat cgaacggcct gacaaatatc
    14941 atagcgcgcc tcccgaaact ggatctcgcg gcgatcggcc cgccgccgga gcgcccggac
    15001 ctgacgccgc cggcccagcc ggtgaggccg aatgtggacc tccgcgctcc ggaaaaccgg
    15061 gccatagcgc tgcgcttcat cgaggatttc aaccgcttca aacggcaggt cgaggacctg
    15121 aaatatcgtg ctgtccgata ccagcgcgaa gtggaacggt accgggcgcg cgttgccgag
    15181 ctgcaggcaa gcacggcggc gcagttccgg gggatactcg aagaagcgag aacacaatcg
    15241 caggagtttt cccgggtggc gaccgaaacg cagaggtatc ggatggccat cgaactggtg
    15301 gctgatcgga cgcggaacgc atacgagcgc gatattgcgt tcggcgattc cgtgggttac
    15361 gccgagaagg accgccgggc cgtcagggac agtgcgctgg ccgcaaatca ccggatttcc
    15421 cttaccttgg attctctgta cagcgaagct gcggcaacaa gacggtactg ggaagaacgg
    15481 cgcgattcaa cggaaggcgg cgaggcctca cctgcattcc aggtgatcaa taatctcgtt
    15541 acgcagtacg cggcagtcgc tgattcgttc cgggtgacgg cagttcgaag ctacatgtac
    15601 acgtacaaag gacgcgattc aatcttcacc gtcggcctcg atcatccgat tgttcagtac
    15661 gcgatccagc gtctccgggt gattgatccg acctttggcg tccagacggt gccgttgacg
    15721 ttcacctacg tcaccgagga ctccacaggt ctgtggcggg tcagcagcac catcgaccgc
    15781 cagaacccgg atgcgtggag gcaggtcgcg ttcaatgaca gcacgtgggt ccctgccgtc
    15841 aagggaacga tgccgcgcgt tgagtttgtt caggctgccg atacaaccgc cggtgcagcg
    15901 ggacagccag aaccggcggc cggcgaaggc gctgtcacag cggaaggcga tacgacggcc
    15961 gaggctgctc cgcaggtggc ggaagttccc aagatcgtcg gtttcccgcc tgacaacctg
    16021 aacatgacgg acctctggtc gccgacgtat gccgacacgg tgtatttccg ttacaagctg
    16081 cagttgcctc cacggtggga tgagctgccc gactcgttgt ccgaaccggg gcggaagcgg
    16141 ccggtggtgc gcaaagcaag tatcacgatt actgcggacg acgattatgt ggtctacgtg
    16201 aacggcgagg tgacgccggc gcgcgacact gatgcacggg tagactggaa aaccgcgcgc
    16261 acgtatgagg tgttgaaaga ccagtggaca gtcggcgaca ccgcaaacat tattgcgata
    16321 cgcgcgcgca acgagaagcg cctgaatcgt attcccgaca cggataccac gacgtacggc
    16381 gtcatggcac gtatcgacat cgagatggac gtgccgctgt atatttacga agcgctgtac
    16441 cgcccaccgg aaccggaacc aaccttcgtg ctggcgttta cgccggacga ctcggtgaaa
    16501 atggccgata ccaccggtac ctacttccga acggctgctg agcgcgagcg gtggcaggaa
    16561 tgccgcacgc gtaccctccg ggctgcgtgg gaggacacgg tgctgattcc atggcgcgct
    16621 caacgcgatg ccctgaagaa ggcgcaactg gactccgaaa tcgttcggct cacccgctgg
    16681 atccgtcagc ggcaggaaga agcacgcggt cgtatcgcaa ccgcggcggc ccgcgaagcc
    16741 gggttggaag aatcgggtga atcacccgct cccgacgcgg gtactgattc cccgcaggaa
    16801 accccggcgc ggcccgaaga cacaggcgga ccataggagt tgaacgtcat gagaaattca
    16861 cgtctggttg cgttgtccat ctccgcgctg tgtgcggttg tgttgctggc tcccctcgcg
    16921 ggcgcgcagg aagcagtgcg aacggtccga gcggcgggga caatccggga aaaacctccg
    16981 ggggaattgg cgaccgtagt gcggcgggat ccagattttc gcacgatcaa atacttggga
    17041 atgcagcggg attaccgcga tgaattgcgc tccaaattga tgctcgggca tgcggttatc
    17101 cggcacggac gtcccgtcct gcctatggag ccaatcgtgt ggccgcccga accggcgc
//
LOCUS       DAOTNG010000021        17096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3340_length_17096_cov_5.015962, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000021 DAOTNG010000000
VERSION     DAOTNG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3340_length_17096_cov_5.015962"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..267)
                     /locus_tag="P5179_01980"
     tRNA            complement(191..267)
                     /locus_tag="P5179_01980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:His,seq:gtg)
     gene            complement(385..1188)
                     /locus_tag="P5179_01985"
     CDS             complement(385..1188)
                     /locus_tag="P5179_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS94018.1"
                     /translation="MLVWQNMHTHTHRSNCGGRDSTIANTVAEAESVGLTLIGVCDHI
                     DEPGSGREPLIVENMKERDELDTSVRVLIGSEVSMNAPDLPAVEPEIMATWDYVIISA
                     NHFHLPCVSQPDERTEEGYADHFLTMAEGAVRYGASIIPHPFSYVGVRTMEGGREIDW
                     PRLMAAYDMKRVRRLFAEAAAKGTAFEINPGRLGIMPEFFRKIIRMGRDEGMKFSLGS
                     DGHHPGQMHYGGPEKIAEIESQLRNLGVLETDICSNYAPRACTARAGTV"
     gene            1292..2479
                     /locus_tag="P5179_01990"
     CDS             1292..2479
                     /locus_tag="P5179_01990"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRS94019.1"
                     /translation="MTVSGALLIDHFVRQWAELHEREWELTPLHTLTEKARHALGTDG
                     CRAALALLSVWNQHQPKFPTWRHLAADRQSAEQASGERAARWRAGRLSGCTRILDLCC
                     GIGGDAIALSSHCEHLVAIDRDFQRALCARFNVSSFGHGDRTDVVCSDVGAVLPAAEA
                     AVLDPDRRPGGRRVIRPELYEPPACLWAQIRKSVRTLAVKVAPGIPFDAIPADAVPEF
                     VEDRGECREAVLWFGERKPHARTAVILGVGSTVTASCIDASAPESFQKGEVGRFVYDP
                     SPAVVRAQLVTHLAAKLSAWRLDDRIAYLSSDSLVETPFARGFEVLSVIPFSLKRLRA
                     HLREKRVGAVEIRTRRFPVNPDELRPMLDLRGPESATVILTRVQGNALAIVCRQGNSG
                     GLI"
     gene            2563..>3023
                     /locus_tag="P5179_01995"
     CDS             2563..>3023
                     /locus_tag="P5179_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94020.1"
                     /translation="MNAEYAWVEGRPVARVDSLGINAFLTDWLVLDPFPFELPGGLNR
                     ELWIGRADAVDADPTGVERTVRPREGDKQRNDAVAGGFSVWQRLIGSTVHDLKSRYPG
                     FSSALVYAATYLHADTDTILAFCIDDWEIYRSCTAQLLLDGVEVCFGPSPAV"
     assembly_gap    3024..3033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3034..7281
                     /locus_tag="P5179_02000"
     CDS             <3034..7281
                     /locus_tag="P5179_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HRS94021.1"
                     /translation="PAGTHCLIAKIGGGSGSGFAWKLSVRAGRVETTPAGWGVSLARL
                     PGFWRGEECAPEVEVDVVLVNPTGSPLEAGKIAANLCGQTQGAPSENHFLDPWQAKAI
                     RISAPLGACSPESTTTVELAFYGRTVAFPVTVPRLLPPCTIHVLEGFHCDPVWVSDQR
                     HYNLISLENVKMQVDACLVDPGYRAFVHELDYLKSFVDEYPDYRAKVFELVASGQLIV
                     GSSYSEPNENNISGEAIIRNIIYGQGYHRHFLGGDPRVYHAWDVFGHIPQLSQILARS
                     GHRAALWSKSILGFPPLFRHMSLDGTVLPHIRTHYTWYSNSLDNLRTISEPVVREKQS
                     FGWHRHLMVEASDFTPPSAWMIGRTAEMAASYPRILMTGPEEFLDGLTADGARLPLTS
                     RNPSQHHVGTFHTRVELKLANRLAENMLFAAEIWSTFAGMLGAEYPDHLIDKAWRQLL
                     FGQHHDAITGTPCDVSYLDLMAGYREALRLARGVLEDATTYIADAVTVSRDGTSVVAF
                     NPLAWERDGVITIQKPEGFEGIEVRNQDGTSLPCFANETTVEILAKRIPSVGYSTLEL
                     LPAPARSEPTVTRCYVLENEYWRMELDPARGGGIVSLFDKEQGKEIVDSALGVGNDLL
                     ALGNGPNPWEFHTTGERVAASDSPAVVEVRTTPTSQTAVVSGRLGEICSYRRTLTLRP
                     GSRTIEASVILTNYRANGHQFVATFPTALQGALPIFEDKFGSVVARRGKVRYDYRTSG
                     SHRASDCAIFPVYNWVEAGWSATAEVPGTGAEGRLNIGMMGIIIPHDSAVEDAAEPLL
                     KALNRVGVTSTFYFDDDDEPRKKELDLNGYGELFYHHLSDDTTQERVRDLAMAAQWMA
                     VSVAGENAYVEQLLARLPGEVSRSLSAQCDAGGWGVVLVEDDRVPEAWGTMPVIVISA
                     VSREAAAAAFRAISQSLDTISSIQLPAGTDFRTTKTPLDDYGFAVLTSGTGAATMEPD
                     GTLTLFLGRTSDWAQNNMIGREINPERRDMAFHYGFYGHAGSWRDGNVVRAGYEFNNP
                     LTAVVPSRSTVARLPKTQSFLSLTGTGAVVTAVKPAGNPLARFENTPSDPRNGIVVRA
                     YEALGRSERIELRLFSGIARASETDLVENDVTSVPVDGGAVARELAPFSINTFRVIPV
                     EKDRSGGVQPSVRDWRPDHVVWCRYYQHNVGAHPTGYLPVGIYIVGELPIENRGGQFP
                     TVGRCRVWIVNNNTDRAISGTARLLAPDHWTLHPAEIPYELAPREHLTTEVVVAFDRR
                     PRVGMIKARMEYDGVVYQDVLEAGFKTVSRGVATERGAVRYNGWEVVKEREPEWTVLR
                     NAEDIIARIRNPWLQPLEAEVAVISPMEMWGRDAGSYGLCALEPDTAAMTLRPRETRE
                     IRFRVSVGEKPRPTFWAWAKLMCNGKCAYLPVPGTTA"
     gene            7399..7471
                     /locus_tag="P5179_02005"
     tRNA            7399..7471
                     /locus_tag="P5179_02005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7432..7434,aa:Met,seq:cat)
     gene            7989..8192
                     /locus_tag="P5179_02010"
     CDS             7989..8192
                     /locus_tag="P5179_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRS94022.1"
                     /translation="MATGTVKWFNHTKGYGFIRPESGEDLFVHVTNVDQADAKRISDG
                     DAVEFDVRPGRKGQEAYNVRLAH"
     gene            8372..9250
                     /locus_tag="P5179_02015"
     CDS             8372..9250
                     /locus_tag="P5179_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94023.1"
                     /translation="MIRRTRIGVLYGALVLALVAGGCNIFGSSEKEPGSEAARYQQEL
                     ADFLALNSTSTISSDVLLALAKESRQAAGIGILQISRELSQSSTSSSTSFPFDSLSAA
                     ERAEKLANLQAMDQALALLLYANMAQAGTGPKVAVDPLSDTYLQQKARNVNLSGTFDV
                     CSVYPDFLVVRILRLMIEFRTRLDAIGPDFRVVGVTGGYRVQYTGAGTVPTAKAIAVN
                     NAIDWLTNPTNGVLSVQINAILDPQSAFGCESPLSDIIDLNKVRSYINTVVVASLKRS
                     KFPVAKPAAEDVVVGE"
     gene            9271..10356
                     /locus_tag="P5179_02020"
     CDS             9271..10356
                     /locus_tag="P5179_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94024.1"
                     /translation="MKRALTFASLIAASVAVVPSVSSASVPHSFRHEVFALGMGGAYT
                     AGAWRGSALFYNPASVAAKRFHMNVPVRFEFGGVGGLTQMKDVVNFFKDNKSDLERID
                     QLPSWRVAELDREAEKLDGKGGTIRAFPAIRLGWRGVGIQSYAVFDGTPQMNSGVFQP
                     RLDLNAYSDIGVIAAYGRPVNLGMLWYAGASVKYFNRWQTIRSFSLEDAVNTPGLGDV
                     VDLKNRQTGIGVDLGALVPVTRKVTLGVVAQDAVTMGDVNPDMSLNLGVHYRVLPRLN
                     LVADYRDLLNASDVSVPMHVHMGGELDLTLLRLRGGFYQGYPTAGVGVNLWLIKLDAV
                     YYGRERGSKLGYEPEDNVAVELQIGLD"
     gene            complement(10425..11255)
                     /gene="rfbD"
                     /locus_tag="P5179_02025"
     CDS             complement(10425..11255)
                     /gene="rfbD"
                     /locus_tag="P5179_02025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRS94025.1"
                     /translation="MIVGSGGMLARELARQWSSRHELIALTRQELDVSALENVRGAVR
                     AHAPDVVVNCAAYNRVDDAETHVEDAYRVNALGPRNLAIAAEEQNAALVHFSTDYVFD
                     GKKTTPYLEYDAVNPLGVYARSKVAGETAVRDHCTRFFLVRVAWLVGHGGRNFVEAML
                     HAAKEKGAVSVVTDQVGTPTFCADVARALHTLLETRAFGLYHITGNGSCSWFEFAEEI
                     FRQADLNVSVTPTTMSAYARPAPRPASTILRNLMLELTVGDTMPPWQESLRQYLSTRA
                     "
     gene            complement(11290..12183)
                     /locus_tag="P5179_02030"
     CDS             complement(11290..12183)
                     /locus_tag="P5179_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94026.1"
                     /translation="MKFLPAFRRFLVVVLLLSPATGYSWGYKAHQMITAAAIDILPAD
                     MQVVLAPYRAALVAQSHAPDVWRHDPDERPRHYVDLELGDRSGYPFPNFPRDYATALR
                     RLGPDSLKKMGTLPWHVDAYFRRTVSSLADPNDSTLVFLSALGHYVADAYMPMHTTVN
                     YDGQLSGNRGVHLRFEWWMIDQNATAIWMKPGLPARITDPLENTWQIVMASYTDVDTI
                     LRADTAVRQSFRPPIPGPRGERSADSPYDQELWNRIGWLAQFRMNLASSAVAGFWYEA
                     WLRAGKPSLQGLSALPPPPMD"
     gene            complement(12180..12653)
                     /locus_tag="P5179_02035"
     CDS             complement(12180..12653)
                     /locus_tag="P5179_02035"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRS94027.1"
                     /translation="MTLISNSSNFAARLLDARYVWVSRIVLAVVFLVYGASKIPNLDD
                     FAHSIANYQLLPETLVNALAITLPWIEVIAALALLFRRWAPGAVVAVGGMTLVFTVAI
                     ISALLRGLDINCGCLDTGTTADARNNLLQHLVFDLFLLALAAHVWWWARRNEATR"
     gene            complement(12650..13168)
                     /locus_tag="P5179_02040"
     CDS             complement(12650..13168)
                     /locus_tag="P5179_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRS94028.1"
                     /translation="MVKKAVIQACLICAGASLIGVGVNLVAPWGIPFVARGRNLPQLP
                     DSALVKGTDPAPSDGHELVSITLQQAKTLFDRDQAVFVDARPEFEYAEGHIPGAINIP
                     FDEVEYYGSAIASLDKHKPLVVYCAGESCDLSIHLGELLAKKGFTAVRVFFGGSVAWE
                     RAGFPFDRGTGG"
     gene            13333..14028
                     /locus_tag="P5179_02045"
     CDS             13333..14028
                     /locus_tag="P5179_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRS94029.1"
                     /translation="MNAQSVLTIILTAGVFLMSGCREMDAELKQHVVAHWSFAVPVTE
                     KLADLAGKHDALVEGVRASEGGGAWYFPGDTAALRVANTPELDLVNGFTMEATIRLER
                     LGNPRVDIGVQGILEKHIGKGGWLLIVLRDGRVGYWLETEQGRTEGASTIALTPGTWH
                     HVAMTWDKQKIRLYVDGKPAGEQSFAGPLGRCPQDLYLGNDDTKNWSFCGDIADIRIL
                     DIAVEPPAFHRAR"
     gene            14223..15650
                     /locus_tag="P5179_02050"
     CDS             14223..15650
                     /locus_tag="P5179_02050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRS94030.1"
                     /translation="MPTEKLDPSLLSDVLRAALRRGADFADIYAEQAAYASVESDDRK
                     INSSFVQDGGVGIRVVKNNRTYYGISASEEPADLMALARQVADAAEAEAARETSVIVV
                     EGSTNWPSSFSVPPDMLDVQQKLAIVRLGEEAAWSGEKRVRQVSIGYRDSVRRVQLAS
                     SVTGTVLEKELGLTEFSARVFVGDGNQLFVGTAGRSIFGGPEHFQGIDSFESITLRAR
                     RNGITQLEADDAPRGMMPVVFAPGDNGVLFHEACGHGMEADLVERGSAFAGKQGELVA
                     SELVTLYDDGTIPCFPGSFPFDDEGIASQRTTLIERGVLRNYLQSLVTARKSACEPTG
                     NGRRQNYRHPPIPRMRNTYIGAGSMNPEDIIAQTDAGLYAEVHGSGGQVDVITGRFTT
                     SVQVAWLIEKGKRTRPVRGATLSGTGIDVLKNIDMVGNDLVIAQMSGRCGKGQSVPVG
                     VGMPTVRVRSLLVGGKGEVWKGATP"
     gene            15647..17008
                     /locus_tag="P5179_02055"
     CDS             15647..17008
                     /locus_tag="P5179_02055"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRS94031.1"
                     /translation="MNDMEFARRTLHYAMQRGVQAAEVAVVRTRKTEISVVNMTPETV
                     NAQEEIGFGVRVLKDARMGFAASNRLDSRSVQDLLDSLVSIVGEHTPDEANTLPEWSG
                     GKPWEDRLSTFDEVILRTPLEEKVRRALTLEQCARDFDTRIKGASTVIYGDVAEEEAL
                     VNSRGVEGVSRSTLAYAVIEAIAGDKDSIETGWHVGASSTYDGLDIEATAATAARRAL
                     RMVGSRPAQSAELPLVFPPEVGSDILAYISRMLDADAVQKGKSLFRGREDSQVASSLV
                     SVVDDGRLEGGLATRLADAEGVPTARTELVRDGVLKGFLYDSYTAKKGGTVSTGNAQR
                     GSYAARPHTGTTNFMLLPGSRATDTLLHDVSIGLYVAEMQGLHAAINPVSGDFSIPCK
                     GLMIRGGELAEPVTGVAISGNLFDLLRDVDGVGDDFTWVFSGAIVGTPTFRVRNVKVG
                     SAG"
BASE COUNT         3473 a         4715 c         5372 g         3526 t
ORIGIN      
        1 caggcggggc ctggttcagt ctcctgagaa tctgcgtgct gcgtgccatt ggcgatctcc
       61 cgttcacccg gctggcgcgc gccagtctcg gggacgcgaa aaaaaggatc ggcgcgtttc
      121 cgtgccgatc ctcgtttcgt acgttgaagg agccccggac gcggaagcat ccgaggccct
      181 tactatcgta tggggtgagt gaggggattc gaacccccga ccctcagatc cacagtctga
      241 tgctctaacc aactgagcta cactcaccat ggttttccgg ttgtgcacat ggacggtttc
      301 ctgaactgcc gtcgcccgtg gcagcaggaa gacagctagt taataatagc gaacgaaact
      361 caccgagtca acgaaaccgg cgcgtcagac tgtgcccgct cgcgcggtgc aggcccttgg
      421 ggcgtaattg ctgcagatat ctgtttccaa cactcccagg ttcctcagct gtgactcgat
      481 ttccgcgatt ttctcaggcc cgccatagtg catctggccg gggtggtggc cgtcggaacc
      541 caacgagaat ttcattccct cgtctcgtcc catcctgatg atcttccgga agaattccgg
      601 catgatcccg agacggcccg gattgatctc gaatgcggtg cccttggcgg cggcctccgc
      661 gaacagcctg cgaacacgtt tcatgtcgta tgcggccatg agccggggcc agtctatctc
      721 ccgcccgcct tccattgttc ggaccccgac gtacgaaaag gggtgcggga tgatcgaagc
      781 accgtaccga actgcaccct ctgccattgt aaggaaatgg tccgcatatc cctcttcagt
      841 ccgttcgtcc ggttggctta cgcacgggag gtggaaatga ttcgcagaga tgattacata
      901 atcccatgtc gccatgattt ccggttccac cgcaggcagg tccggtgcgt tcatggagac
      961 ttcagagcct atgagcacgc gaacggaagt gtcgagttcg tcgcgctctt tcatgttctc
     1021 gacgatcaga ggttcccgac cgctccccgg ttcgtctatg tgatcgcaga cgccaatgag
     1081 cgtcaagccg accgattcgg cctccgcgac ggtattcgcg atggtgctgt ctcgccctcc
     1141 gcagttggaa cggtgtgtgt gcgtatgcat attttgccat acgagcattc gatgcccttt
     1201 cggttgcagg cggatcgaca cgtggtactc tccaaatggt aaacctggac agttccctcg
     1261 cacaacgact cccgctgaat ggcgtggcct cgtgacggtc tccggggcgc tcctgatcga
     1321 tcattttgtc cggcagtggg cggaacttca cgaacgcgag tgggaactca cacctctcca
     1381 tactcttacg gagaaagcac gccacgccct tgggacggat ggttgccggg ccgcgcttgc
     1441 cttgctttcc gtatggaacc agcatcagcc gaagtttccg acgtggaggc atctggcggc
     1501 cgaccggcag agcgctgaac aggcgagcgg cgagcgggct gctaggtggc gcgcggggcg
     1561 actatccggg tgtacgcgca ttctcgacct gtgctgcgga atcgggggcg atgccatcgc
     1621 tctttcgtcg cattgcgagc atctcgtggc gatcgacagg gattttcagc gggcgctctg
     1681 tgcccgtttc aacgtgagtt cattcggcca cggcgaccga accgatgtcg tttgctcaga
     1741 tgtaggagcg gttctaccgg cagcagaagc cgccgtcctt gatcctgacc ggaggcccgg
     1801 cggcaggcgg gtgattcgac cggaactcta cgaaccgcca gcgtgcctat gggcgcagat
     1861 ccgtaaatct gtccggaccc ttgccgtgaa ggtggcgccc ggaatccctt ttgatgctat
     1921 tccggcggat gcggtccccg agttcgtgga ggaccgcggc gagtgccgag aggcggttct
     1981 ctggttcggc gagcgaaaac cgcacgcaag gaccgccgtc attctcgggg tgggctccac
     2041 ggtgacggcc tcctgcatcg acgcttccgc ccctgagtcc ttccagaaag gtgaggtcgg
     2101 aaggttcgtg tacgacccgt cgccggcggt tgttcgcgcg caactcgtca ctcatctcgc
     2161 ggcgaagctt tccgcgtggc ggctggacga tcggattgcc tacctgtctt cagattcgtt
     2221 ggtggagacg ccatttgcga ggggttttga ggttctttcc gtcataccct tcagtctcaa
     2281 aagattgaga gcgcatctgc gcgagaagag agtgggcgcc gtggaaataa ggacgcggag
     2341 gttccctgtg aatcccgatg aactgaggcc gatgttggac ctccgtggac cggaatctgc
     2401 aacggtgatt cttacccgtg tgcaaggaaa tgcactcgcg attgtctgca ggcaagggaa
     2461 ttccggcggt ctcatttgag cagacccctg cggtcagata attgcgatgg gaacgccttt
     2521 atttgtgttc gatgcaaaga gtcataccaa caggagtcgc gcatgaatgc cgaatacgcg
     2581 tgggtagaag gtcgcccggt tgccagggtt gattcgctgg gcatcaacgc gtttctcacc
     2641 gactggcttg ttctcgaccc gtttccgttc gaattgcccg gcgggctgaa ccgggaactc
     2701 tggatcggca gggcagacgc agttgacgct gatccgaccg gcgtggagag gacggttcgc
     2761 ccgcgtgaag gggacaagca acgcaacgat gccgtcgccg gcggcttctc ggtctggcag
     2821 cgacttatcg gttccacagt ccatgatttg aaaagccgtt atccgggctt ttccagtgcg
     2881 ctggtgtacg cggcgacgta cctccacgca gacaccgaca cgattctggc cttctgtatc
     2941 gacgactggg agatatatcg ctcgtgtacg gcacagttgc tcctcgatgg cgtggaggtg
     3001 tgcttcggtc cttcgcccgc cgtnnnnnnn nnnccggcgg gcacgcactg ccttattgcg
     3061 aagatcggcg gcggcagcgg gagcgggttt gcctggaagc tttcggtgag ggcgggacgt
     3121 gtggagacca cacctgccgg ctggggtgtt tcactggccc gattgccggg gttctggcgt
     3181 ggcgaggaat gcgctcctga ggtagaggtt gacgtggtgc ttgtcaaccc gaccggttcg
     3241 cctctcgaag caggcaaaat tgccgcgaac ctttgtggac aaactcaggg agcgccgagc
     3301 gagaaccatt tcctcgaccc atggcaggca aaagcaatca gaatcagcgc cccgctgggc
     3361 gcgtgctcac ccgagtccac gacgaccgtt gaactcgcct tttacggcag aaccgttgca
     3421 ttcccggtaa cggtaccccg gctgcttcct ccctgcacga tccacgtgct ggagggattt
     3481 cattgcgacc ccgtctgggt gagcgatcag agacactaca acctgatttc tctggaaaac
     3541 gtgaaaatgc aggtggatgc gtgtctcgtg gacccgggat atcgcgcgtt cgtgcacgaa
     3601 ctcgattact tgaaatcgtt tgtggacgag taccccgatt atcgcgccaa agtgttcgaa
     3661 ctcgttgcgt cgggccagct gattgtgggc agttcgtaca gcgaaccgaa tgaaaacaat
     3721 atctccggcg aagcgatcat ccggaacatc atctacgggc aaggttacca ccgccatttt
     3781 ctgggcggtg acccgagggt gtatcacgca tgggacgtgt tcggccacat tcctcaactc
     3841 agccagatac ttgcgcgttc cgggcaccgc gccgcgctat ggtcgaaaag catccttggc
     3901 ttcccgccgc tgttccgtca tatgtcgctt gatggaacgg ttctgccgca cataaggacg
     3961 cactacacgt ggtacagcaa cagcctcgac aacctgcgga ccatctcgga accggttgtt
     4021 cgggagaaac aatcgttcgg ctggcaccgg catctgatgg tggaggcttc cgatttcacg
     4081 ccaccgagtg cgtggatgat cggcagaacc gccgagatgg ccgcgtcata ccccagaatt
     4141 ctcatgacgg ggccggaaga gtttctcgac gggctgacag cagacggggc gcgccttccg
     4201 ctgacgagcc gcaatccctc gcagcatcac gtgggaacat tccacacgcg agtggaactc
     4261 aaactggcaa accggcttgc agagaacatg cttttcgccg cggaaatctg gtctacgttt
     4321 gccggtatgc tcggggcgga gtatccggat cacctgattg acaaagcgtg gcgtcagctc
     4381 ttgttcggtc aacaccacga tgccatcacg ggaaccccct gcgatgtttc ctacctcgat
     4441 ttgatggccg gataccgcga agccctcagg ctcgcacggg gagtgctcga agacgccaca
     4501 acgtacatcg ccgacgcagt tacggtttcg cgggacggca cttcagtcgt cgcattcaac
     4561 ccgctcgcgt gggagcgcga cggcgtgatt acgatccaga aaccggaggg gtttgaaggg
     4621 atagaggtca ggaatcagga tgggacgtcc ctgccttgtt tcgccaacga gacaacggtc
     4681 gagattctgg ccaaaaggat cccgagcgtt ggctattcga ctctggaact cctgcccgcg
     4741 cctgcgcgat ctgaacccac ggttacccgg tgctatgtgc ttgaaaacga atactggcgt
     4801 atggaactcg atcccgcgcg tggtggggga atcgtgagcc tgttcgacaa ggaacagggc
     4861 aaagagatcg tcgatagtgc tctcggagtc ggaaatgatc ttcttgcact cggcaatgga
     4921 cccaatccgt gggagtttca cacgaccggt gagcgggttg cggcatccga ttcacccgcc
     4981 gttgtagaag tgcgtaccac gccgacttcc cagaccgcag tggtttccgg cagactggga
     5041 gagatttgtt cgtaccgccg cacgctgaca ctgcgccccg gaagcagaac gatagaagcg
     5101 tccgtaattc ttaccaatta ccgcgccaac gggcatcagt tcgtagcgac ttttccgacc
     5161 gcgttgcagg gcgcgcttcc gattttcgaa gacaaattcg gttccgttgt tgcgcgcagg
     5221 ggcaaggtgc gttatgacta ccgaacttcc gggtctcatc gtgcctctga ttgtgcgatt
     5281 ttccccgtgt acaactgggt cgaggccggt tggtcggcaa ctgcggaggt accgggcaca
     5341 ggtgccgagg ggcgtctgaa catcggcatg atggggataa ttatccctca tgattccgcg
     5401 gtcgaggatg ccgctgaacc tttgctcaag gcgctgaaca gggtcggtgt cacctcgacg
     5461 ttctatttcg acgacgatga cgaaccgagg aagaaggagc tcgacctcaa cgggtacggt
     5521 gagctgttct accatcatct ttccgacgac actacccaag aacgagtgcg tgatctggca
     5581 atggcagcgc aatggatggc tgtttcggtt gccggtgaga acgcgtatgt cgaacagctt
     5641 ctcgcccgtc tccccggcga ggtgagccgt tcgctctccg cacagtgcga cgcgggtggc
     5701 tggggagttg tcctggtgga ggatgatcgg gttcccgagg catgggggac gatgccggtg
     5761 attgtgatat cggcagtatc gcgggaagct gcggcggcag cgttccgtgc catcagccag
     5821 agtctcgaca ccatttcgag catccagctt cccgcaggaa cggatttccg caccacgaaa
     5881 acgccacttg acgactacgg ttttgccgtt ctgacctccg gcacgggcgc cgcgaccatg
     5941 gaaccagacg gaacgctcac tctgttcctc ggtagaacat cggattgggc tcagaacaac
     6001 atgattggga gggaaatcaa cccggagcgg cgggacatgg cattccatta cggattctac
     6061 ggtcacgcgg gatcgtggcg cgacgggaac gtggtacggg cagggtacga gttcaacaac
     6121 cccctaacgg cagtggtccc gtccaggagc actgtagcgc gtcttcccaa gacccagagt
     6181 tttctgtcgc ttaccggtac gggagcagtg gtgaccgccg tgaaaccggc aggcaatcct
     6241 ctggcgcggt ttgagaacac accgagtgat ccccggaacg ggatcgttgt ccgggcgtat
     6301 gaggcccttg gaaggtccga aaggatcgaa ctgagattgt tttcgggcat agcgcgcgcg
     6361 tcggagacgg atcttgtgga gaacgatgtc acctccgtgc cagtggatgg gggtgcggtt
     6421 gcgcgcgagc tcgcgccatt ttccatcaac accttcaggg taattccggt tgagaaagac
     6481 cgaagtgggg gtgttcagcc ttccgtccgt gactggcggc cggaccacgt ggtctggtgc
     6541 cgttactacc agcacaacgt gggagcgcac ccgacagggt atctcccggt cggcatctac
     6601 atcgtcgggg aacttccgat tgagaaccgg ggcgggcagt tcccgaccgt cggacggtgc
     6661 agggtgtgga tagtgaacaa caacacggat cgcgcgattt ccggtacggc ccgcctgctg
     6721 gcacccgacc attggacgct tcatccggcg gaaatcccgt acgaattggc tcctcgagag
     6781 cacctgacaa cggaagtggt ggttgccttc gaccgcaggc cgcgcgtcgg catgataaag
     6841 gcccgaatgg aatacgatgg agtggtatac caggacgttc tggaagccgg gttcaaaacc
     6901 gtgagtcgcg gagttgctac cgaacgggga gcagttcggt acaacgggtg ggaagtggtc
     6961 aaagagcggg aaccggaatg gacggttctg cggaacgcgg aggatattat cgctcgaatt
     7021 cggaatccat ggctccagcc gctggaagcg gaggttgcgg tcatcagccc gatggaaatg
     7081 tggggtcggg acgcggggtc gtacggtttg tgtgccctgg aaccggatac ggcggcgatg
     7141 acattgcgtc cgagggaaac gcgcgagatt cgtttccgtg tcagcgtcgg ggaaaagccg
     7201 aggccgacgt tctgggcgtg ggcgaaactc atgtgcaacg ggaaatgtgc gtatttgccg
     7261 gttcccggca cgaccgcgtg atggtggtaa tcgtggtttt tcaggaatcg cagaacggag
     7321 ctgtgtgttc ggcgcacgat tttcttccat tcgggtgttg acatccgatg cccgggtgcg
     7381 tatacttaac tgtcccaacg cggggtggag cagttggtag ctcgccaggc tcataacctg
     7441 gaggtcggag gttcaaatcc ttcccccgct accttgatca caacacctgt cgcattgtta
     7501 tgtcgcgcga cggagagtat agggaacgcg taaggttgat cacgaccgct gtttgcagtc
     7561 cttgggttgc ttcatccctc gggttcctga cgatctatga tcatgctcct gcgtacgcct
     7621 gtgccgtgag gtgcgggcgt tcttttttgc agcctgccgc gtccaccaag ccgtggtttc
     7681 cctcgagcgg gttgcatgtt cgcgaagaag gaggtggggg tagagcatag tcgtctgtgg
     7741 ttcgtgtaga gcacgcgttt tggtaaggaa gactacctct ttggacgtgt atcagcgtgg
     7801 aatttgtcca accgtcgtcg gtatccggca gaacggagca ggttcacgtt gacagttcga
     7861 tcacaagggg gaaggctgcc aaaagtcccg cgtgagcggg gtagtaagtc tgtggtcgag
     7921 aacacgcagg gctggagccc tgtgcactcg tcacgcaacc tgggcaatgc ccgaggaaag
     7981 gtcagcaaat ggcaactgga acagtgaagt ggttcaacca caccaagggt tatgggttca
     8041 tccgtccgga aagcggtgaa gacttgtttg tgcacgttac gaacgtggac caggccgacg
     8101 ccaagcggat ttccgatggc gacgcggtgg aatttgacgt tcgtccgggc cgcaagggtc
     8161 aggaagcata caacgtccgt ctggcgcatt aagccctgac gaatctgcgc aatgcgcccg
     8221 tccttgactt ttcaggggcg ggcgtgtttt tttgtacgca gttcgtctca acaatagtcc
     8281 atttgtctct ctattgtatt tgagagcgat gccgacgacg gttctggcga agctccaaac
     8341 tcgaacgcac gatgtgccgg agggatgtca catgattcgt cgtacacgaa taggagtgtt
     8401 gtatggggcg ctggttcttg cgctggtggc cggaggatgt aacatcttcg gctcatccga
     8461 aaaagagccg ggctccgaag ctgcgcggta tcagcaggaa ctggcggatt tcctcgcgtt
     8521 gaacagcacc agcacgatat catctgatgt gctgcttgcg ctcgccaaag aatcgcgcca
     8581 ggccgcggga attggtatcc tgcagatttc gcgcgaattg agccaaagca gtaccagttc
     8641 gagcaccagt ttcccgtttg acagcctttc cgcggcggaa cgcgcggaaa agctggccaa
     8701 cctgcaggca atggaccagg ctcttgcgct gctgctgtac gcgaacatgg cgcaggcagg
     8761 gacgggtccg aaagttgccg tagacccgct ttcagatacc tatctgcagc agaaagcgcg
     8821 aaacgtgaac ttgagcggaa cgtttgacgt gtgcagcgtg tatcccgatt tccttgtggt
     8881 gcggatcctg cgtcttatga ttgaattccg gaccaggctc gatgcaatcg ggccagattt
     8941 ccgcgtggtg ggtgtaacgg gtggctacag ggtgcagtac accggggcag gcacggttcc
     9001 gactgccaaa gcaattgccg tgaacaatgc aattgactgg ttgaccaacc cgacgaacgg
     9061 cgtgctgtcg gtgcagatca acgcgatact tgatccgcaa tcagccttcg ggtgcgagtc
     9121 gccactgagc gacatcatag acctgaacaa ggtccggtcg tacatcaaca cggtggtggt
     9181 tgcgagcctg aagcggtcga aattcccggt agcgaagccc gccgccgaag acgtcgtggt
     9241 tggcgagtag acacgtaagg agaaccagca atgaagcgcg cattgacttt cgcatctttg
     9301 attgcggcct ctgttgcggt ggtgccttcg gtgtcctctg ccagtgtacc gcacagtttt
     9361 cgtcacgagg tgtttgcgct cgggatgggt ggagcctaca cggctggagc gtggcgcggg
     9421 tcggcgctgt tctacaaccc ggcgtctgtt gccgcgaagc ggttccacat gaacgttccg
     9481 gtgcggtttg aattcggagg cgtcggcggc ctgacccaga tgaaggacgt cgtgaacttt
     9541 ttcaaggaca acaagtcgga cctcgaacgg attgaccagt tgccttcctg gcgagtcgct
     9601 gaacttgacc gggaagcgga gaagctggat ggcaagggcg gcacgatacg ggcattcccc
     9661 gcgatccgcc tcggctggag aggagtgggg attcagtcat acgccgtttt tgatggcacc
     9721 ccgcagatga actccggcgt gttccagcca cggctggatc tgaacgcgta cagcgacata
     9781 ggggttatcg ccgcgtacgg taggccggtg aaccttggga tgctctggta cgccggcgcc
     9841 agcgtgaagt acttcaaccg gtggcagacg attcggagct tctcgctgga ggacgcggtc
     9901 aatacgccgg gtcttggtga cgtcgtcgac ctcaagaacc ggcagaccgg tatcggcgtg
     9961 gaccttggtg cactggttcc ggtcacccgc aaagtgactc tcggagtggt tgcgcaggat
    10021 gcggtcacaa tgggtgatgt caacccagac atgtcactga acctgggcgt tcattaccgg
    10081 gttctgccac ggctgaacct cgtggctgat taccgtgacc ttctgaatgc gtcggacgtc
    10141 tcggtcccca tgcatgttca catgggtggg gagctggatc tgaccctgtt gaggcttcgg
    10201 ggtggtttct atcaggggta cccgaccgcg ggtgtgggtg tcaacctctg gctgatcaag
    10261 ctcgatgccg tgtactacgg ccgcgaacga gggtccaaac ttgggtacga acctgaggac
    10321 aacgtggccg ttgagcttca gataggtctt gattgaccct gatttgtcct gaccaaagat
    10381 gaagtgtccc ccgcggcata tcgcggggga cacttttttt cgaatcaggc gcgcgttgac
    10441 aggtattgcc tgaggctttc ctgccaggga ggcatagtgt cgccgaccgt cagctcgagc
    10501 atcaggttgc gaaggattgt cgaggcaggc cggggcgccg gcctggcgta cgcagacatc
    10561 gttgtcgggg tgacggaaac attcaggtct gcctgccgga atatttcctc cgcgaattcg
    10621 aaccacgagc acgaaccgtt gccggtaatg tgatagagcc cgaatgcgcg cgtttccagg
    10681 agagtatgaa gcgctcgggc gacgtccgca cagaacgtgg gtgttccgac ctggtcagtg
    10741 acaacagata ccgccccttt ttcctttgcc gcgtggagca tggcttccac gaaattcctt
    10801 cctccgtggc caacaagcca cgcgacccga acgagaaaaa aacgcgtaca gtggtctcgt
    10861 accgcagttt cacccgcaac cttgctcctt gcgtataccc caagggggtt gaccgcgtca
    10921 tactccagat agggtgttgt cttcttgccg tcgaagacgt agtcggtgga aaaatgaacc
    10981 agcgcggcat tctgctcctc agccgcgatg gcgagattcc ttgggcccag cgcgttcaca
    11041 cggtaggcgt cttccacgtg ggtttcggca tcgtcaacgc gattgtacgc cgcgcagttg
    11101 actaccacgt caggagcgtg cgccctcact gcgccgcgca cgttctcgag cgcggacaca
    11161 tccagttcct gcctcgtgag cgcgatgagt tcatgtcgcg atgaccactg ccgggcaagc
    11221 tcgcgagcga gcatcccgcc ggagccgact atcatgattt tcacggcact actcccgcgc
    11281 agcgtacgct caatccatgg gcggtggcgg caatgcggaa agcccctgta acgaaggctt
    11341 tcctgcacgc agccacgcct cataccagaa acctgcgact gccgaggagg caaggttcat
    11401 tcggaactgc gccagccagc cgatgcggtt ccacagctcc tgatcatacg gcgaatccgc
    11461 ggatcgttcc ccgcggggtc ccggaatagg cggccgaaac gactgccgta ccgcggtgtc
    11521 ggcgcggagg atggtatcaa catccgtgta ggaagccatc acgatctgcc atgtattctc
    11581 gagcggatcc gtgatccgag cgggaaggcc cggtttcatc catatcgccg tggcgttctg
    11641 gtcgatcatc caccattcga agcgcaaatg caccccacgg tttccggaca actgcccgtc
    11701 gtagttcacc gtcgtgtgca tgggcatgta cgcatccgcg acataatgcc cgagggccga
    11761 caggaacacg agcgtgctgt cgttcgggtc ggcgagggaa ctgacggtac ggcggaaata
    11821 tgcgtcaacg tgccagggaa gagtgcccat tttcttcaga ctgtccggtc ccagtctccg
    11881 caacgcagtg gcatagtcgc gagggaagtt gggaaagggg tatcccgaac ggtcgccgag
    11941 ttccagatcc acgtagtgac gtggtcgctc atccggatcg tggcgccaca cgtccggtgc
    12001 atgagattgt gcgacaagcg ccgcacgata cggtgccagt acgacttgca tatcggcagg
    12061 cagaatgtct atcgcggcgg cggtgatcat ctgatgggct ttgtatcccc acgaatagcc
    12121 agttgccggg gacagcaaga gcacgacgac aagaaatctg cgaaatgccg ggagaaactt
    12181 catcgggttg cctcgtttct gcgggcccac caccagacat gtgcggccaa tgcgaggagg
    12241 aaaaggtcga acacgaggtg ttgcaacaga ttgttgcggg catccgccgt tgtgccggtg
    12301 tcaaggcacc cacaattgat atccaggcct cgcagaagag cgcttatgat cgcaaccgtg
    12361 aaaaccagag tcatgcctcc aactgccacc acagcgccgg gtgcccaccg gcggaacaaa
    12421 agcgcaagcg ccgcaatgac ctctatccac ggcagagtga tagcgagcgc gttgaccaga
    12481 gtttccggaa gcaactggta gttcgcgatg ctgtgggcaa aatcgtcgag gttcggtatc
    12541 ttgctcgcgc cgtacacgag aaagactacc gcaaggacaa ttctggacac ccaaacgtat
    12601 ctggcgtcca gcaaccgcgc cgcaaagttc gatgagttcg aaatcagagt catcctccag
    12661 tcccgcgatc aaatggaaaa ccggcgcgct cccacgcgac cgaaccgccg aaaaagacgc
    12721 gcactgcggt gaatcccttt tttgccagca attcgcccag atgaatactc agatcgcacg
    12781 attcccccgc gcaatagaca acgagaggtt tgtgtttgtc aaggctcgcg attgccgagc
    12841 cgtagtattc gacttcgtcg aagggaatgt tgatagcgcc ggggatgtgt ccttctgcgt
    12901 attcaaactc aggcctggca tcaacgaaga cggcctgatc acggtcgaaa agtgtttttg
    12961 cctgctggag ggttattgaa acgagctcgt gaccatcgga aggcgccgga tcagtccctt
    13021 tgaccagcgc cgagtcgggc agttgaggaa ggttcctccc ccgcgccaca aacgggattc
    13081 cccatggtgc aacgaggttc actcccactc cgatgagcga cgctccggcg caaatcaggc
    13141 atgcctggat cacagccttc ttgaccactc tgattctccc gctgcactat tgaaattgta
    13201 tattcaccgc attatcatgg tagccgctcc ggtgcggtgt gtcaatcaac cgcacgcaat
    13261 tcgttgcctt ggcgccgtca cggtacggca tgtatcatca aattccgtca aacgcgtcgg
    13321 gaggaagggc ggatgaacgc gcaatcggta ctgacaatca ttttgacagc aggagtgttt
    13381 cttatgtccg ggtgcaggga aatggatgcc gaattgaaac agcatgtagt ggcgcactgg
    13441 tcgttcgcgg tgccggtcac cgagaaactt gccgatctcg cgggaaaaca cgacgcactg
    13501 gtggaaggtg ttcgtgcgag cgaagggggt ggtgcctggt attttccggg ggacacggcc
    13561 gcgctcaggg tggcgaacac gccggagctg gaccttgtga acgggtttac catggaagcc
    13621 accatcaggc tggagcgcct cggaaatccc cgggtcgaca tcggtgttca agggatactt
    13681 gagaaacaca taggcaaagg cgggtggttg cttatcgtac tgcgggatgg ccgcgtcggt
    13741 tactggctgg aaacagaaca gggtcggaca gaaggcgcca gtacgatagc tcttacgccg
    13801 ggcacgtggc accacgttgc gatgacatgg gacaaacaga aaatccggtt gtacgttgat
    13861 ggaaaacctg ccggagaaca gtcgttcgcg gggccactcg ggcgatgtcc gcaggatctt
    13921 tacctcggga atgatgacac gaaaaactgg agcttctgtg gcgacatcgc cgatatccgc
    13981 atcctcgata tcgccgtcga accaccggcg ttccacagag cccgttgaat gccgagctcg
    14041 aagacggaac ccgtgcccgg atggtgttct cggtccagcc ggggttaccg ggtggctgca
    14101 ccgaacgcga caaccttcac aatcatcgac accgggcgcg cctattttcg ctacccgtgc
    14161 ccgttcggaa gaagagagca ctgacgcgtc ccgttccacc agttttcgtc aagaacccgc
    14221 cgatgcctac cgaaaaactt gaccccagtc tcctgagtga cgtgttgcgc gcggcgttac
    14281 gccggggtgc cgatttcgct gacatatatg cagaacaggc cgcatacgcg agtgtcgaaa
    14341 gcgacgacag gaagatcaac tcctcctttg tgcaggacgg aggtgtgggc attcgcgtgg
    14401 tgaaaaacaa tcgcacctat tatgggatct ccgcttctga agaacctgca gacctgatgg
    14461 cactggcgcg ccaggtggca gacgccgcgg aggcggaggc agcgcgcgaa acgtccgtca
    14521 tcgttgttga gggctcaacc aactggccat cgtccttctc cgttcctccg gatatgctcg
    14581 atgtgcagca gaaactggcg attgtccggt taggcgagga agcggcatgg agtggcgaga
    14641 agcgagtcag gcaagtctcc atcggctacc gtgattccgt gcgccgcgtt cagcttgcgt
    14701 cctcggtgac aggcaccgtg ctcgaaaagg aactgggact taccgagttc tccgcacggg
    14761 tcttcgtcgg ggacggaaac cagcttttcg tgggaactgc cgggcggtcg attttcggtg
    14821 gccctgagca tttccagggt attgacagtt tcgaatcaat cacgttgcgg gcccgccgga
    14881 acgggatcac ccagctcgaa gccgacgacg ctcccagagg tatgatgccg gttgtgtttg
    14941 caccggggga caacggagtg ctgtttcacg aggcgtgcgg acacgggatg gaagctgacc
    15001 ttgtggagcg aggatcggca tttgcaggaa aacaaggtga actggtcgcg agtgagcttg
    15061 tcacgctgta cgacgacgga accatcccct gctttccggg gagcttcccg ttcgacgacg
    15121 agggaattgc ctcgcagaga acaacactga ttgagcgcgg cgtgctcagg aattacctcc
    15181 agtcgcttgt cacagcgcgg aaatccgcgt gcgaaccgac gggaaacggg agacgccaga
    15241 actaccgtca tcctccaatt ccccggatgc gaaacacgta tatcggcgca ggctccatga
    15301 atccggagga cataattgcg caaacggacg cggggcttta cgcggaggtt catggttctg
    15361 gcgggcaggt tgacgtgatt accggcaggt tcaccacgag cgtgcaggtg gcgtggctca
    15421 tagagaaagg gaagcggacg cggcccgtac gcggagctac cctgagcggt accgggattg
    15481 acgttctgaa gaacatcgac atggtgggga atgatcttgt catcgcgcag atgtcgggca
    15541 ggtgtggaaa ggggcagagc gtgcccgtgg gcgtcggtat gccgacggtg agggtcagat
    15601 cgctgctcgt tggtggcaaa ggagaggtct ggaagggagc cacgccatga acgacatgga
    15661 gtttgcccgc cgaacactgc actatgccat gcagcgaggg gttcaagctg cagaagtggc
    15721 cgttgtgcga acccggaaaa cggaaatcag tgttgtcaac atgacaccgg agacagtgaa
    15781 tgcccaggag gagatcggat tcggtgtcag ggttctgaaa gacgcgagga tggggttcgc
    15841 cgcgagcaat cggctggatt cgcgttccgt ccaggacctg ttggattcgc tcgtgtccat
    15901 cgtgggtgaa catacacccg acgaagcaaa cacgctgccg gaatggtccg gcgggaaacc
    15961 ctgggaagac aggctttcaa ccttcgacga ggtgatcctt cgtaccccac ttgaggaaaa
    16021 ggtgcgcaga gcgcttaccc tcgagcaatg cgcgcgcgat ttcgacacgc ggatcaaagg
    16081 cgcgagcacg gtgatttatg gtgacgtggc ggaggaggaa gcactcgtca actcacgcgg
    16141 ggtggaggga gtgtcccggt ctaccctcgc gtatgcggtg atcgaggcaa ttgccggcga
    16201 taaggattcc atagagacag gctggcacgt aggggcaagc tccacgtacg acggtctgga
    16261 catcgaggcg accgccgcga cggctgcgcg tcgtgcgttg cgcatggtgg ggagcagacc
    16321 cgctcagtcc gccgaactcc cgctcgtgtt tccgccggaa gtcggcagtg atattctcgc
    16381 gtacatatcg aggatgctgg atgcggacgc agtgcagaag gggaaatcgt tgttccgggg
    16441 gcgggaagat tcacaggtgg cctcttccct cgtttccgtt gtggacgacg ggcgtctcga
    16501 gggtggcctt gcaacccgtt tagcggacgc agagggtgtg ccgactgcgc ggacggaatt
    16561 ggtgagagac ggagttttga aaggctttct gtacgattct tacaccgcaa aaaaaggcgg
    16621 cacggtttcc acgggaaacg cccagagggg aagctatgcc gcgcgtcctc ataccgggac
    16681 gaccaatttc atgttgttgc ccgggagtcg cgcgacggac acattgctgc acgacgtgtc
    16741 gatcggtctg tatgtagcgg aaatgcaggg gttgcatgcg gcgatcaacc cggtatccgg
    16801 cgatttttcc attccctgca aggggctcat gatccgtggc ggagagctcg ccgagcccgt
    16861 gacgggtgtg gcaatcagtg gtaatctgtt cgatctgttg cgcgacgttg acggagttgg
    16921 cgacgacttc acgtgggttt tctccggtgc gatcgtcgga acacctacgt ttcgcgtgcg
    16981 gaacgtgaaa gtcggcagtg caggttgatg ggtggcgtgg aacataccag gtggatcggg
    17041 atgacaatga caaacgtact ctggttggtg attttctggg cgatgcaggt ggttgc
//
LOCUS       DAOTNG010000022        17015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3362_length_17015_cov_5.239682, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000022 DAOTNG010000000
VERSION     DAOTNG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17015
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_17015_cov_5.239682"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1170)
                     /gene="galK"
                     /locus_tag="P5179_02060"
     CDS             complement(4..1170)
                     /gene="galK"
                     /locus_tag="P5179_02060"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRS94032.1"
                     /translation="MSGPTSVAEKACALFRATWRRGPDCLASSPGRVNLIGEHTDYND
                     GFVFPVAINRRTFVAMGASRDGRSGFVSTSHPIPVLADCPPWRRMENECWANYVAGVA
                     AQFANRAECVPALYVSVASDLPAGTGLSSSAALEAATAAALSQLVGASFSITELATLC
                     QKAEHEFAGVRCGIMDQMSSVAGGAIVLDCRSLAFQRVQIPTDLSLVILDSGIPRGLS
                     ATRYNERRGQCEEGVRVLKGRFSGIKALRDVTPGMLDACRKDLSETVFRRCRHVVTEN
                     VRVSAAAKAFAENDFPAVGKLLQESHVSMRDDYEISLPEIDLLVEIASSAPGCYGARL
                     TGAGFGGAVISLVDTGAADVFAEEVVEKYRTKTCKAGTAMRVVPDEGSRKEEPV"
     gene            complement(1173..1862)
                     /locus_tag="P5179_02065"
     CDS             complement(1173..1862)
                     /locus_tag="P5179_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRS94033.1"
                     /translation="MDAEESLSNPLSPEEEEQVREEARKRLLAENEHRNAGNGVAPNG
                     VDVHAELRRAHRLRVMREEEDAFYAERGLFRYRNHRGETEWLTQDEIAKRKANHRRKR
                     SSLIRRLQRQLPSGEFFGFLAVIGLLVAGFLYFRDGRAPAFRTALEIRVMSNPQGAAV
                     FLDGVSANATTDAILPVRKAGPHVISVARPGFRAVPSSVIVNLTEGSPQGLATFTLYP
                     VRSDTTNGMAE"
     gene            complement(1852..3426)
                     /locus_tag="P5179_02070"
     CDS             complement(1852..3426)
                     /locus_tag="P5179_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS94034.1"
                     /translation="MILGAMAVFLAICAPKVMAAARGFYARLTILSTIVRLARDVYVD
                     EVDTDRLMDGAIDGLLDRLDPHSDYLPPEEAARFGERIRGSFGGVGIYYTIIEGLPTV
                     TSVIEGGPAEHAGLLSGDRILSVDGLTTVNWREAEVQEHLKGPVGTRVRLEIRRNGRE
                     TPLPVEIVRGVIPLKSVPYAFMLDDTTGYIRIANFAQTTGQEVEEAIGRLNDAGMRCL
                     VLDLRDNGGGELNAAVAVADFFLDAGSVIVSQRGRWEQANQTYFATSGSRKVDTPVVV
                     LINRGSASASEIVAGALQDTDRGIIVGQQSFGKGLVQRQFDLSQQAQGGGVLLLTVAR
                     YYTPTGRLIQRDYGAGTAQYILSGIGEGVRVDTSQGPRFVTPLGRVVYGKGGISPDYA
                     IPRNEPDPVLAKLYSSALFFRFVNDAANTVVVTPQRGEEFRASQLVDDRVLGEFFDFC
                     ERMDSSFTKLKLEAEENSIRPHLAAAIAEKTWGQQGRYRVLAPFDSELSAARAHLVDA
                     RSLLNRSRSGRAHIGR"
     gene            complement(3471..5147)
                     /locus_tag="P5179_02075"
     CDS             complement(3471..5147)
                     /locus_tag="P5179_02075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS94035.1"
                     /translation="MVESMRGENARSIEENAHSAPSLDLSVVILSYNVRAYLALAIRT
                     ALQATRSLAAEIIVVDNASQDGSADMVAADFREVRLIRSDRNLGFSGGNNLGIRIARG
                     RYILLLNPDVIVHPRAFEALVAYLDTHPSAGAAGGRVVNPDGTTDRGARRGFPSPSAA
                     FYRMVGLSFIFPKSRRFARYNMTYHDDGQECEVDAVSGCFMAVRREVVERIGLLDESF
                     FMYGEDLDWSFRIRSAGWTIGYVPSAEIVHFRGESTRSIARLRQLYLFHRAMHVFVRK
                     HIAPTRIPLLVWLIEFGIVVRGAAITLWRVLLAAVVPIADVAVLTAGFVLALFARTQT
                     GWTIPPFSIRQWATIWMVFVASGLLGAAATGLYVRNRLGLRRAAVAAAIGAVASIITI
                     FLVRTINFSRIVTLVTWALGGGGMLLWRWLVSRASQPPRAGWLVLGCGEKAQRFLNSL
                     SSSEDRYHVLGVVRSEDDPIDCTAVAGFPVVGSASDLPILLRSFRVDDLIVAKEKYHY
                     HELLDLVRRGGRYPGRVRLVADDDVPVGAETPPDNRFMDLNLQKRTWFLQ"
     gene            complement(5140..5874)
                     /locus_tag="P5179_02080"
     CDS             complement(5140..5874)
                     /locus_tag="P5179_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRS94036.1"
                     /translation="MTEGPAGVVVIPTYNERENLERLVDEIRSLNLNLDVLVVDDNSP
                     DGTGSLAERLRSEGRVAHVIHREGKLGLGSAYVAGFRWALAKNYPLIFQMDADFSHDP
                     HALPDFMSAIEHADLVIGSRYINGITVINWPIGRLLLSYFANAYARFVTRMPLRDMTG
                     GFKCFRRDALKRINLDRISSEGYSFQIEMNFIALHLGFRVKEIPIVFRDRQAGTSKMN
                     FRINREAAWMVWKLRLLAWAGRLGHG"
     gene            complement(5889..7091)
                     /locus_tag="P5179_02085"
     CDS             complement(5889..7091)
                     /locus_tag="P5179_02085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS94037.1"
                     /translation="MQCMAANTPELERSYAEVAGRAFKRPGEMRDHILKWMKRPRYSH
                     AHTRIVVDNGAVVGGLAIVELPVRYGDTVLKLGGISCVATDPESQKRGYGRANMEDAV
                     AYMAQNGYDLSFLLGIRDYYYRYGYRTAIVWFECRISPNVYSAKPAPGLRVRRARKSD
                     IPGMAALYEKNGAQCDLSVVREKKDWLWYFDFGRFSDAHVVVNSEKNIVGYLVCRGAG
                     RVDEIGLEDTTEVYETVLAFLQEKAKQAFSTQVTVYTPGENGFARYCLYKKRSTWARW
                     TDYQGGPMLRLFNVENLFRKIAPTLQIRWQRVPRGLRPVAFTVQCPMGQVAFAPTETD
                     LTIKPGAVAGEVVVLPDEALTELVLGFRPAADVVADAGARPAAATVALLDAIFPVSTT
                     FLSPTDHM"
     gene            complement(7112..7648)
                     /locus_tag="P5179_02090"
     CDS             complement(7112..7648)
                     /locus_tag="P5179_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HRS94038.1"
                     /translation="MEGNAYNEAIFAPDLCSPDAPPLDPSQVRIFRDPHGYLRATIGD
                     RTYLDVTITRAFPLQASDRYICILTGRLGEVGVIENLADLDEESRRIASEELDRRYYL
                     PRIQRIKSIREEFGATYWVVETDFGERSFVGKGVRDNVHYVNSERLLIQDVDGNRYEV
                     YDVNALDPASRSLLFRVT"
     gene            complement(7740..8501)
                     /locus_tag="P5179_02095"
     CDS             complement(7740..8501)
                     /locus_tag="P5179_02095"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRS94039.1"
                     /translation="MQVDNSPVTVSTEKPLFFFSDAHIHTRTTRDEAEKQDALVAFLR
                     AIKAEAGGVFVVGDLFDFWFEYGAVVPRHGTRVMVALAELVDAGIPVICLGGNHDWWL
                     GNALTEEYGVYVHRCPLWVRAQEQTIFLAHGDGLSGPDRYYRLVRAVLHNRISEAAFR
                     VLHPSCGAVLANLASRTRSNREYCDTVQAQITPVFRAAVPEVFARGASIAVFGHVHTV
                     RLELVDNNVLAVLGDWINLRSYGELREGAFRIRTW"
     gene            8636..8863
                     /locus_tag="P5179_02100"
     CDS             8636..8863
                     /locus_tag="P5179_02100"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRS94040.1"
                     /translation="MPKTPGWADSLREVAPLLSLGIQLALIVVASVIIGWWVDQRYGT
                     GPWGVLIGAVFGTGSGLYHFIRTALGMKDRH"
     gene            8877..9323
                     /locus_tag="P5179_02105"
     CDS             8877..9323
                     /locus_tag="P5179_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94041.1"
                     /translation="MTLEKETGTLLRTWALWFFGLTILSALLSAYPLLISSSLHRMSA
                     MIGWGVSFLVCVVSGYLAFWSLGRSHNTFLLVALGGIVLRLALISGAVFVGISLLHLH
                     TVIFLGSLLGSYTMYHGLEIIVLNQSKALVRTPGGSGAVARGNEAH"
     gene            9323..10327
                     /gene="atpB"
                     /locus_tag="P5179_02110"
     CDS             9323..10327
                     /gene="atpB"
                     /locus_tag="P5179_02110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRS94042.1"
                     /translation="MVFPRVRLVAKTFVLVAILSCPALAADSHASTADTAVVGAHGQG
                     GVAAAAHGGGHGNQVDAIHHVVDAREIEIPWPNRDLAKVIHLPSLGTFHIGSIPVDMA
                     ITKHVVFLWVAAILLFVVVSVAAAKRSRGTDPPRGITNLIEIFVAFIRDEITVPSLGP
                     HYGRVLLPYFLTAFFFILFCNLLGLVPYGSTATGNISVTAALATLSFFVIQGVAISSM
                     GFGGWIKHLTGGVHWALWPIMIPVEIMGLFTKPFALCVRLFANMTAGHVVILSILGLI
                     FIFRSVAVAGLAVPFSVFIYLLELLVAFIQAYVFTMLSAVFAGLGVHSSHDSEEAEAH
                     "
     gene            10415..10642
                     /locus_tag="P5179_02115"
     CDS             10415..10642
                     /locus_tag="P5179_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRS94043.1"
                     /translation="MEALSLTHFAAGIGAGIVTFGAAFGIGKIAQAAMDGTARQPEAG
                     GQIRISMLIAAALIEGIAMFGQVICVLLAIK"
     gene            10769..11263
                     /gene="atpF"
                     /locus_tag="P5179_02120"
     CDS             10769..11263
                     /gene="atpF"
                     /locus_tag="P5179_02120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRS94044.1"
                     /translation="MLVSVNPGLIIWTIITFLVLVFLLSKFGWKPIVGALENREQAIR
                     QAIAEAHQARDEAQKILEAQNKLIASAEAEAREIVQAARETSEQIRREADVTARTESQ
                     RLLDQARREIENAKELALRDIRDVVADLAVAGASKILKETLDPERHRAMVDDLVASLE
                     KSKA"
     gene            11303..11872
                     /gene="atpH"
                     /locus_tag="P5179_02125"
     CDS             11303..11872
                     /gene="atpH"
                     /locus_tag="P5179_02125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRS94045.1"
                     /translation="MAIHSRVSIRYARALVLAAQEADNIASLRQETEGLTELMQRTPS
                     LVKFLDDPSINKHDKSRILNAVFGDHLSPLMRRFVTMLVGGYRERLLPDILAAVLTLL
                     DAREGRVHAEVASATPFADAQPERLRHALEGLTGKKVTMATREEPDLGAGFVVRVGDT
                     VFDASLRAQLAQMRAQLLQSNLRPAVRTS"
     gene            11947..13485
                     /gene="atpA"
                     /locus_tag="P5179_02130"
     CDS             11947..13485
                     /gene="atpA"
                     /locus_tag="P5179_02130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRS94046.1"
                     /translation="MAVTFDVRPDEVSQILAQQLAGFERGVDVYEAGTVLQVGDGIAR
                     VHGLSNVMAGELVEFPHGIMGLAFNLEEDNVGCILFGETTEIKEGDTVKRTKRILQVP
                     VGEALLGRTVDALGSPLDGKGDIVTDRYDFVERKAPGVIQRQPVKTPLQTGLKAIDAM
                     TPIGRGQRELIIGDRQTGKTALAVDTIINQKGQNVFCIYVAIGQKASTVANVVGILES
                     HGAMDYTTVVSAPASTPAPLQYLAPYSGCTMGEFFRDSGRDALIIYDDLTKHAWAYRQ
                     VSLLMRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSLTALPIIETQEGDISTYI
                     PTNVISITDGQIFLESDLFYGGQRPAINPGISVSRVGSKAQIRAMADRDVAGRLRTDL
                     ARYNEVAAFAQFGSDLDAVTQQTLRRGERLQEILKQGQYVPMPVEEQVLSLFAATTGA
                     LDELPVHSVRQWEREYLENMRAAHADVLKEIREKRTIPAPLKEKIREITKQFTKTFKP
                     AGTA"
     gene            13530..14393
                     /gene="atpG"
                     /locus_tag="P5179_02135"
     CDS             13530..14393
                     /gene="atpG"
                     /locus_tag="P5179_02135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRS94047.1"
                     /translation="MPNLRMIRRRINAVNNIQQVTRAMKMIAASRMRKAQENILVARP
                     YVRRLETLLRHIASRIPQGAHPLLDVRPVKKALLIVVAGDRGLCRGFNANAIRRTVER
                     YGELKAQGVECSLIAVGRKPYEYFGKRGFPIAASHTGIFQHLNFLDAQEIASEATKLF
                     LNRDADLVEIVYNEFKSVIQQVVVLERFLPVEPIAPEDGDMPCHYLYEPSLEAIWSSI
                     VGKFVNLEIWRVLLESNAAEQSARMTAMEEATKSAEEMTKRLIGIRNQVRQTNITTEL
                     TEIVGAANALE"
     gene            14481..15881
                     /gene="atpD"
                     /locus_tag="P5179_02140"
     CDS             14481..15881
                     /gene="atpD"
                     /locus_tag="P5179_02140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRS94048.1"
                     /translation="MNVGKVVQVMGAVIDVDFTGAELPAILNALEIDRENGERLVLEV
                     AQHLGENKVRTIAMDATEGLVRDMPVRDTGVPISVPVGKRTLGRMLNVIGEPIDGKGD
                     LGTGKRLPIHRPAPLLTSLTVKAEMFETGIKVIDLVQPIMRGGKVGLFGGAGVGKTVL
                     ITELIHNIAKEHGGYSVFAGVGERTREGNDLFLEMGESGVLERTALVFGQMNEPPGAR
                     ARVALTGLTLAEDLRDESGVDVLLFIDNIFRFVQANSEVSALLGRMPSAVGYQPTLGT
                     DIGELQERITSTKQGSITSMQAIYVPADDYTDPAPVATFAHLDAVTYLDRAIVEQGRY
                     PAVHPLRSTSRILDPLVVGEDHYNVARQVQQVLQRYEDLRDIIAILGMDELGDDDKMA
                     VLRARKIQMFLTQPMFVAEAFTGTPGKYVPVVETVRGCREILEGRHDDLPEEAFRMVG
                     TIDEAVERAERLRSGS"
     gene            15881..16324
                     /gene="atpC"
                     /locus_tag="P5179_02145"
     CDS             15881..16324
                     /gene="atpC"
                     /locus_tag="P5179_02145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRS94049.1"
                     /translation="MPGTFSLEIVTPTRILFQGEVEMLRAPGTLGSFQVFPGHAPLIS
                     TLEVGEIDIRLPGGKERAIATSGGFVEVLRKGVTVLAETAEFAEEIDKARAEQAVARA
                     KQQLEHLRTTARVKAEAATRQVSDAEVEMALIRALNRLRVAQREH"
     gene            16468..>17015
                     /locus_tag="P5179_02150"
     CDS             16468..>17015
                     /locus_tag="P5179_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94050.1"
                     /translation="MNEISDEQTGARVVRLTPPAGLHYHQYCYGQWIAPDCKTLLVFR
                     YRELRRDSPIDLYRVNTDGKGYALVAENAGWSTISVDGQFVFAGTAGGILRIPLSGGE
                     SDTVFRSDSYRGMSANAQTRDGRFLFCHAFGERGHELVRVQLDTGEAKVLFQTDYLMH
                     LHLHDEGQGRLLATVRPPDLEWG"
BASE COUNT         3599 a         4896 c         5020 g         3500 t
ORIGIN      
        1 tgactacacc ggttcttctt ttctgcttcc ttcgtcggga acgacgcgca tggcagttcc
       61 agccttgcac gtttttgtcc gatatttctc gacgacttcc tccgcgaaca cgtctgccgc
      121 acctgtgtcc accagagaaa tcaccgcgcc cccaaaaccc gctcccgtga ggcgcgcccc
      181 gtaacatccc ggggcggaag aggcgatttc cacgagaaga tcaatctcgg ggagacttat
      241 ttcgtagtca tcccgcatgc tcacgtggga ctcctggagc agcttcccga ctgctggaaa
      301 atcgttttcg gcgaaggctt tcgcagccgc ggaaacgcgt acgttctcgg ttactacatg
      361 ccggcacctc cgaaacactg tctccgacag gtcctttcgg caggcatcga gcattcccgg
      421 cgtgacatcc ctgagtgcct ttatcccgga aaatcgtcct ttcaacacgc gtactccttc
      481 ttcacactga cctctacgct cgttgtagcg cgtggcggag agcccgcgcg ggattcccga
      541 gtcgagaatg acgagtgaca ggtccgtggg aatctggacg cgttgaaacg caagtgatct
      601 gcagtcaagg acgattgcac ctcctgcaac gcttgacatc tgatccatta ttccgcaacg
      661 cacgcctgcg aattcgtgct ctgctttctg gcaaagggtg gcgagttccg ttatcgaaaa
      721 cgaggccccg accaactggc tcaacgcggc agcggttgcg gcctccagag cggccgaact
      781 gctcagcccc gtaccggcgg gcagatcgct tgccacagac acgtacaacg caggtacgca
      841 ttcggcgcga ttcgcgaact gggccgccac gcctgcaacg tagttggccc agcattcatt
      901 ttccatgcgc cgccacggtg gacaatcggc cagtactgga atcgggtgcg aggtggagac
      961 aaaccccgag cgcccgtcgc gcgatgctcc cattgccacg aacgtgcgtc tgttgatcgc
     1021 caccgggaag acaaatccgt cgttgtaatc tgtatgctca ccgatcaggt tgacacggcc
     1081 aggagacgaa gcgaggcaat ccggccccct ccgccaggtc gcccgaaaaa gagcacacgc
     1141 tttttctgca actgaagttg gtccggacat cgttactcag ccatgccgtt tgtggtatcg
     1201 gaacgaacag gatacagcgt gaatgtggcc agcccctgag gagacccttc ggtgaggttc
     1261 acaatgacgg acgagggaac tgcccgaaaa cccggacgtg cgacagaaat cacgtgaggc
     1321 ccggcttttc gcacggggag aattgcgtcg gttgtagcgt tcgctgacac cccgtccagg
     1381 aagacggcgg caccctgagg gtttgacatc actcttatct ctagggcagt ccgaaacgca
     1441 ggagcgcggc cgtcacggaa atacaggaac cccgcgacaa gaagcccgat gaccgccaag
     1501 aaaccgaaaa actccccgga aggcagttgc cgctggaggc gccggatcaa actcgacctt
     1561 ttcctgcgat ggtttgcctt tctcttcgcg atctcatcct gggtgagcca ttcggtctct
     1621 ccccgatggt tcctgtaacg gaacagtccg cgctccgcgt agaaggcgtc ttcttcttcc
     1681 ctcatgacgc gaagacgatg ggcgcggcgc agctcagcgt gtacgtcgac gccattcggg
     1741 gcaactccgt tgcctgcgtt ccggtgttcg ttctcggcca gaaggcgttt acgcgcctcc
     1801 tcgcgtacct gctcctcttc ctccggagaa agagggtttg aaaggctctc ttcagcgtcc
     1861 aatgtgcgcc cttccagaac gagaacggtt gagcagcgat cgtgcatcta caagatgcgc
     1921 acgggctgcg gaaagctcag agtcgaacgg agcaaggaca cggtaacgac cttgctgccc
     1981 ccacgtcttc tccgcgatcg cggccgccag atggggccgt atggagttct cctctgcctc
     2041 cagtttcagt tttgtgaacg aggaatccat tcgctcacaa aaatcgaaaa actcaccgag
     2101 cacgcgatcg tccacaagct gcgaagcgcg gaattcctcc cctcgctgcg gcgttacgac
     2161 tactgtattc gcggcgtcat tcacgaatcg gaaaaacagc gccgaagagt aaagcttcgc
     2221 gaggactgga tcaggctcat tccgcggaat cgcgtaatcg ggcgagatgc cgcctttgcc
     2281 gtaaactact cgtcccagag gtgttacgaa acgcggtccc tgcgacgtgt ccactctgac
     2341 cccttcgcca atcccactca aaatgtactg cgcagtgccc gcaccgtaat cgcgctggat
     2401 cagtctcccg gttggcgtat agtagcgcgc aacagtaagg aggagaacgc cgccgccctg
     2461 ggcctgctga ctcaggtcga actgccgctg caccaacccc ttcccgaaac tttgttgtcc
     2521 cacgatgatt ccgcgatctg tatcctgcag cgcgcccgcg acaatttctg aagcgctcgc
     2581 agatccccgg tttatgagca cgacgacagg tgtatcaact ttccgtgaac cggatgtggc
     2641 aaaataggtc tggttcgcct gctcccagcg cccgcgctga gaaacaatga cggaccctgc
     2701 gtcgaggaag aaatcggcga ctgcgactgc ggcgttcagt tccccacccc cgttgtccct
     2761 caggtccaga accagacacc gcatccctgc gtcgttcagc ctgccgatgg cctcttccac
     2821 ttcctggccg gtggtctgtg caaaattcgc gattcggatg taaccggtgg tgtcatcaag
     2881 catgaaggcg tagggaacgc tcttcagcgg gatcactcct cgaacgattt ccacaggcag
     2941 gggcgtttcc cgcccgttcc gccggatctc gagccgcact ctggtgccaa ccggcccttt
     3001 caggtgctcc tggacctcag cctcgcgcca gttgactgtt gtcagaccat ctacggaaag
     3061 aatgcgatcg ccggaaagaa gcccggcgtg ttccgccggc ccgccttcga tcacagaggt
     3121 taccgtggga agcccttcaa tgatggtgta atagatgccg acgcctccga aacttccgcg
     3181 tatccgttcg ccaaaacgag cagcttcttc cggaggcaga tagtcgctgt gagggtcaag
     3241 acgatcgaga agaccgtcta ttgcgccgtc catcagtctg tctgtgtcta cctcgtcgac
     3301 gtacacatcc cgggccagtc gaacgattgt actgaggatt gtcagccgcg cgtagaaacc
     3361 gcgggcggcc gccattacct tcggcgcgca gatcgccagg aagacagcca tcgcgccaag
     3421 gatcaccgcg ttccttgttc tactgctgcg aaaacaggaa ggcacggact tcattgaagg
     3481 aaccaggtcc gtttctgcag gttcaggtcc atgaagcggt tgtccggcgg tgtttccgcc
     3541 ccgacaggga catcgtcgtc cgcaacaagc cggacccgtc cgggataccg ccccccacgc
     3601 ctcacgaggt cgagaagctc atggtaatgg tatttctctt tcgccacgat caagtcgtca
     3661 acacgaaagc tgcgcaacag gattggaagg tccgatgcac taccgacgac cgggaacccc
     3721 gctacggcgg tgcagtcgat cggatcatct tcgctgcgaa ccacacccag cacgtgatag
     3781 cggtcttccg atgacgaaag ggaattgaga aagcgttgcg ccttttcgcc acaacccagc
     3841 accagccacc cggcgcgtgg cggttgcgat gcgcgggaaa caagccagcg ccagagcagc
     3901 atcccgcccc cgcccagggc ccacgtaacc agtgtcacga tgcgcgaaaa gtttatcgtt
     3961 cggacaagga aaatcgttat gattgacgcg acggccccta tggcagcagc gacagctgcg
     4021 cgacgcagac cgagacgatt ccggacgtac aaaccggtgg cggcggctcc cagcaaaccg
     4081 gaggcaacaa aaaccatcca tatggtggcc cattgccgga tgctgaatgg tggaatggtc
     4141 catcctgttt gtgtccttgc gaacagcgca agcacgaaac cggcggtaag cacggccacg
     4201 tcagcgatcg gaacgaccgc agcgaggagc actctccaga gtgttatcgc ggctccacgg
     4261 acaactatgc caaactcaat cagccacacg agaagaggaa tcctcgtagg tgctatgtgc
     4321 ttgcggacga atacgtgcat cgcccggtgg aacaaataga gctggcgcag gcgagcgatg
     4381 gatcgggtgc tttcccccct gaaatggacg atctccgcag acggtacata accgatcgtc
     4441 caaccggcag accggatacg aaacgaccaa tccaggtctt cgccgtacat gaaaaagctc
     4501 tcgtcgagca gcccgatccg ctcaacgact tccctgcgaa ccgccatgaa acacccggaa
     4561 acagcgtcca cttcacattc ttgcccgtcg tcgtgatagg tcatgttata acgggcgaaa
     4621 cggcgactct tcgggaaaat gaagctgaga ccgaccatgc gataaaaggc tgccgagggc
     4681 gaagggaaac ccctccgcgc accgcgatcc gtggttccgt cagggttgac aacgcggccc
     4741 ccggctgcgc ctgcggaagg gtgcgtgtca aggtacgcga ccagggcttc gaatgcgcgc
     4801 ggatgaacga ttacatctgg attgagcagg aggatgtacc gccccctggc gatcctgatt
     4861 ccgagattgt tgccacccga aaagcccagg ttccgatcac ttcggatcag cctgacttcc
     4921 cggaaatccg cggcaaccat gtcggcgctt ccgtcctgcg acgcgttgtc aactacgatt
     4981 atctcggcag caagggagcg tgttgcctgc aacgcggtgc ggattgccag tgcaaggtat
     5041 gcgcgaacgt tgtagctcaa aatgacgaca gagaggtcaa gggaaggagc gctgtgcgcg
     5101 ttttcttcga tagagcgggc gttctctccg cgcattgact caaccatgcc ccagtctccc
     5161 ggcccaggcc agcaagcgca gcttccacac catccatgcc gcttcacggt tgatgcggaa
     5221 attcatcttg gaagtgcccg cctgtctatc acggaaaaca atgggaattt ctttcacgcg
     5281 gaaccccaag tggagggcga tgaagttcat ttcaatctga aagctgtacc cttccgaact
     5341 gattcgatca agattgatcc gtttcagagc atcgcggcga aagcacttga acccgccagt
     5401 catgtcccgc aagggcatcc gggtaacgaa ccgcgcgtac gcattcgcga aatagctgag
     5461 tagcaggcgg ccgatcggcc agttgataac tgtgatcccg ttgatataac gtgatcctat
     5521 gacaaggtct gcgtgttcaa tagcggacat gaagtccggc aacgcatggg gatcgtggga
     5581 gaaatccgcg tccatctgaa aaatgagcgg atagtttttc gcaagtgccc accggaaccc
     5641 tgcgacgtac gcggagccga gcccaagctt gccttcgcgg tggatgacgt gggccactct
     5701 gccttcggaa cgaagccgtt ccgcgaggct ccctgttccg tctggcgagt tgtcatctac
     5761 taccagaacg tcgagattaa ggttaagaga ccgtatctcg tccacgaggc gttcgaggtt
     5821 ttcccgttcg ttgtacgtcg gaatcacgac gacgccagcc ggcccttcgg tcatgcgctc
     5881 tccgtgtgct acatgtggtc cgttggggaa agaaacgttg tcgacacagg aaaaatggcg
     5941 tccagcaacg ccactgtcgc agcagccggg cgtgcacccg cgtccgccac tacatctgcc
     6001 gccgggcgga agcccagaac cagttccgtg agcgcttcgt caggcaacac gaccacctct
     6061 cccgcgactg ctccgggttt gatcgtcaga tcggtttcgg tgggcgcgaa agctacctgg
     6121 cccatcgggc attgaacggt gaaggcaacg ggccgaagac cccgcgggac ccgttgccat
     6181 cggatctgaa gggtgggagc gatcttcctg aacaggttct ctacgttgaa caggcgcagc
     6241 atcgggccac cctggtaatc ggtccaacgc gcccacgtgg accgcttctt gtaaaggcaa
     6301 taacgggcga acccattctc cccgggcgtg tagaccgtaa cctgggtaga aaacgcttgc
     6361 ttcgccttct cctggaggaa agccagcacc gtctcgtaca cctcggttgt gtcctcaagt
     6421 ccgatttcgt cgactcttcc cgctcctctg catacgaggt acccgacaat attcttctcg
     6481 ctgttcacca cgacgtgagc atctgaaaac cgaccaaaat cgaagtacca cagccagtcc
     6541 ttcttctcgc gtaccacgga aaggtcgcac tgcgctccgt tcttttcgta cagggcggcc
     6601 atccccggaa tgtctgactt gcgtgccctt cgaacgcgga gacccggcgc aggtttcgcc
     6661 gagtacacgt taggagaaat ccggcactca aaccacacaa tcgccgtacg atagccatag
     6721 cgatagtagt aatcccgaat tcccagaaga aaggaaagat cgtacccgtt ctgtgccatg
     6781 tacgcgacgg cgtcttccat gttggcgcgg ccgtatccgc gtttctggga ttccgggtct
     6841 gtcgcgacgc aactgatgcc gccgagtttc agcacggtat cgccgtaacg aactggaagt
     6901 tctactatgg cgagcccgcc gacaacagcg ccgttgtcga ccacaatccg ggtgtgagcg
     6961 tgggagtatc tggggcgttt catccacttg agaatgtggt ctcgcatttc ccccgggcgt
     7021 ttgaaggcgc gaccggcgac ttctgcgtaa ctacgctcca gctccggcgt gttggcggcc
     7081 atacactgca ttgagtgctg tctccctcgg ttcaggttac tcggaaaaga agagaacgac
     7141 ttgccggatc aagtgcattg acgtcataga cctcgtaacg gtttccgtca acgtcctgga
     7201 tgagcagtcg ttcggaattg acataatgga cattatcgcg cactcctttg ccaacaaaag
     7261 agcgctcgcc gaaatccgtt tctaccaccc agtacgtagc gccgaattcc tcgcggattg
     7321 attttatccg ctggattcga ggcaggtaat accgccggtc cagttcctcg gatgcgatcc
     7381 gcctgctctc ttcgtcgagg tcggcgaggt tttcaattac tccgacttca ccgaggcgtc
     7441 cggtaagaat acagatgtaa cggtcgcttg cctgcaatgg gaatgcgcgg gtgatcgtga
     7501 cgtcgagata ggtgcggtcg ccaatcgtcg cccgcaggta cccgtgggga tcgcggaaaa
     7561 ttcgtacctg cgagggatcg agagggggag catcggggct gcaaagatcc ggagcgaaaa
     7621 tcgcctcgtt gtacgcgttg ccttccattc agacacctct gcgccgtggg gaaacgggta
     7681 taaacaaaat gtatggcttc gaacgcccat ccccaacggc ggggcatcaa ctcgttctgt
     7741 caccaggttc ttatgcggaa cgctccctcc ctgagttccc cgtaggatcg gagattgatc
     7801 caatctccca gcactgccag tacgttattg tcgaccaact ccaggcggac tgtgtgcaca
     7861 tggccaaaaa ccgcaatgct ggctcctcgg gcaaaaacct cgggaacagc ggcgcggaac
     7921 acaggggtga tctgcgcctg aaccgtatca cagtattcac ggttactgcg agttctggac
     7981 gcaaggtttg ccagaaccgc cccgcatgac ggatgcagaa cacgaaacgc tgcctcagag
     8041 atcctgttgt gcagcaccgc gcggacaagg cggtagtacc ggtccgggcc agacaagccg
     8101 tcgccgtgcg cgaggaaaat tgtctgttcc tgcgcgcgca cccacaatgg acacctgtga
     8161 acgtacaccc catactcttc cgtaagagca tttcccagcc accagtcgtg atttccaccg
     8221 aggcagatga cgggaatgcc ggcatccacc agctcggcaa gtgcgaccat cacacgcgtt
     8281 ccgtgacgag gcacgaccgc tccatattcg aaccagaaat cgaaaaggtc gcctaccacg
     8341 aacaccccgc ccgcttcggc tttgatcgca cgcaggaagg caacaagagc atcctgtttc
     8401 tctgcctcat cgcgggtcgt tctcgtgtgg atatgcgcgt ctgaaaagaa gaaaagaggt
     8461 ttttcagtgg ataccgtaac gggggaatta tcgacctgca cgtctcgctg gcctccgccg
     8521 ggggttgaca cggattcgac aaaaccagaa ttttgatatt cgccctcacc tatggtgagt
     8581 ccaaatctgc ctgcagtcaa ggcaggtgtc cggggcggcg aacggagtga aatgagtgcc
     8641 gaaaactcca ggttgggccg attcgttacg tgaagtggcg cctctgctga gcctcgggat
     8701 tcagctcgcg ttgatagtag ttgcgagcgt gatcatcggc tggtgggtgg atcagcgcta
     8761 cggaaccggt ccctggggtg tccttattgg agcggtgttc ggtactggat ccgggttgta
     8821 ccatttcatt cgcaccgctc ttggcatgaa ggacaggcac tgaggccgtc cgaagaatga
     8881 cccttgaaaa agaaacgggg actctcctga gaacctgggc gctgtggttt ttcggtttga
     8941 ccattctctc ggctctgttg tctgcctacc cgttgctcat cagttcttcg ctccaccgga
     9001 tgagtgcgat gatcggttgg ggcgtcagtt tcctcgtgtg tgttgtgagc ggatatcttg
     9061 cgttctggtc gctcggccgc tcgcataaca cgtttttgct tgtagcgctc ggcggaatag
     9121 tgctgcgcct tgcgctgatt tccggggcgg tgtttgtggg gatctctctt ctgcacctgc
     9181 acaccgtgat ctttctcgga agcctgctgg gttcctacac gatgtatcac gggttggaaa
     9241 taatcgtgct taaccaatcc aaggcgctgg tcagaacacc tggcggctca ggtgcggttg
     9301 cacgaggtaa tgaggcgcac tgatggtgtt tccgcgcgtt cgcctggtgg cgaaaacctt
     9361 tgttctggtg gcgattcttt cctgtccggc gcttgcggca gattcgcatg catcgacggc
     9421 ggacacagct gtcgttggcg cgcacggtca gggcggagtc gcggcggcag ctcacggcgg
     9481 aggacacggc aaccaggttg acgcgataca tcacgtggtg gatgcccgtg agatcgagat
     9541 tccgtggccc aaccgagatc tggcaaaggt aatccatctg cccagcctgg gcacgttcca
     9601 tatcgggtcc attcctgtgg acatggcgat cacgaaacac gtcgtgttcc tctgggtggc
     9661 ggcgatactg ctgtttgttg ttgtgtcggt ggcggcggcg aagagatcgc gtggaactga
     9721 cccaccccgg ggcattacga acctcatcga aatctttgtc gcattcattc gcgacgagat
     9781 caccgttccg tcccttgggc cacattacgg gcgggttctg cttccgtatt ttctcaccgc
     9841 gtttttcttc attctgttct gcaatttgct ggggttggtt ccatacggtt cgaccgcaac
     9901 gggtaacatc agcgttacgg cggcgctcgc cacactgagt ttcttcgtaa ttcagggcgt
     9961 cgcgatcagc agtatggggt ttgggggctg gatcaagcat ctgacgggcg gagtgcactg
    10021 ggcgctgtgg cccatcatga tcccggtgga aatcatgggg ttgttcacga agcctttcgc
    10081 gctgtgtgtt cgtctttttg ccaatatgac tgccgggcac gtggtaatcc tgtccattct
    10141 cggactgatt ttcattttcc gaagcgttgc ggtggctggt ctcgcggttc cgttcagcgt
    10201 gttcatctac ctgctcgagc ttctggtggc cttcatacag gcgtacgtgt tcacgatgtt
    10261 gagtgccgtc ttcgcgggtc ttggcgtgca cagcagccat gattcagaag aagcggaggc
    10321 gcattgattt gtcggcggca tccgatctgc cgatgcgttg atcacccata gccaacgccg
    10381 cactgcggcg tgatctggtt caggaggagt tttgatggaa gctctcagtc tgactcactt
    10441 tgccgccggt atcggtgctg gaatcgtcac gttcggggcc gcgttcggaa tcggaaaaat
    10501 cgctcaggca gcgatggacg gtaccgcgcg gcaaccggaa gcgggtggac agatccgtat
    10561 ctccatgctg atagccgccg ccctcattga aggtattgcc atgttcgggc aggttatctg
    10621 cgttctgctg gcaatcaagt gatttcaaac cccggcgtca cgtgtaccac ccgaggcagg
    10681 gcgagggaag acacgtttcc gcgccgttct gcttccggtg atgtgaacgt gcgcggggtg
    10741 caggcatcac tttttcccgg ggatatccat gctggtaagt gttaatcctg ggctcatcat
    10801 ctggacgatc atcacgttcc tggtgctcgt ttttctcctg agcaaattcg gatggaaacc
    10861 catcgtcggc gctctggaaa accgggaaca ggcgattcgc caggcgattg ccgaagctca
    10921 tcaggcacgc gatgaagcgc agaaaatcct ggaggctcag aacaagctga tcgccagcgc
    10981 tgaggctgaa gcccgggaga tcgttcaggc agcgcgggaa acgtcagagc agattcggcg
    11041 ggaagccgac gtgactgctc gaacggaatc ccagcgattg ctggaccaag cccgtcgcga
    11101 gatcgaaaac gcgaaggaac tggccctgcg cgacatacga gacgtagtcg ccgaccttgc
    11161 agtggcaggc gccagcaaga tcttgaagga aacgcttgat ccggaacgtc atcgtgcgat
    11221 ggttgatgat ctggtagcaa gcctcgaaaa atcgaaggcg tgaacgttgc cgcgttcgtt
    11281 ccgccggtaa ccagagaaac tgatggcaat tcattctcgc gtgagcattc gttacgcccg
    11341 ggcactcgtc ctcgcagcgc aggaagccga taacattgcc tccctgcgtc aggagactga
    11401 agggctgacg gagttgatgc agcggacgcc gtcgctggtg aaattcctcg acgacccgtc
    11461 catcaacaaa catgacaagt cgcggatact gaacgcagtg ttcggagatc acctctctcc
    11521 actgatgcgg cgcttcgtta cgatgctggt gggtggttat cgcgaacgtt tgctgcccga
    11581 tatcctcgcg gcggtcctga cgctgctgga cgcgcgcgaa ggaagggttc atgcggaagt
    11641 ggcttctgcg acgcctttcg cagacgcaca gcctgagcgc ctacggcacg cactggaagg
    11701 cctcactggc aagaaggtca caatggcgac tcgggaggaa ccggatctcg gcgccgggtt
    11761 cgtggttcgc gtcggggata cggtgtttga cgcgtctttg agagcgcaac tcgcccagat
    11821 gcgcgctcaa cttctgcaga gcaatctgcg tccggcggtt cgcacttctt gagtgtcgcg
    11881 agcggaaact gacacagtgc agagagagca atttcggttg cagcagggga aagaaaagga
    11941 aacacgatgg cggtaacctt cgacgtgcgt ccggatgaag tgtcccagat actggcgcag
    12001 caacttgccg gatttgaacg cggtgttgac gtgtacgagg cgggaacggt gctgcaggtt
    12061 ggggacggta tcgcccgcgt tcacgggctt tccaacgtaa tggccggaga actggtggag
    12121 ttcccccacg gaatcatggg tctcgcgttc aatctggagg aagacaacgt cgggtgcatt
    12181 cttttcggtg aaaccactga aatcaaggaa ggcgacacgg taaagcggac gaagcgtatt
    12241 ctgcaggttc cggtggggga agcccttctg gggcggactg ttgatgcgct tgggagcccg
    12301 cttgacggga aaggcgacat cgtcaccgac cggtatgatt tcgtcgaacg gaaggctccg
    12361 ggagtcattc aacggcaacc ggtcaaaacg ccgctccaga caggactcaa ggcgatcgat
    12421 gcgatgacgc cgattggccg cggacagcgg gaactcatca tcggtgatcg gcagacgggg
    12481 aaaacggcct tggcggttga tacgatcatc aatcaaaagg ggcagaacgt tttctgcatc
    12541 tacgtcgcca tcggccagaa ggcctcaacg gtggccaacg ttgtgggaat cctcgaatct
    12601 cacggcgcca tggattacac cacggtagtt tctgcgcccg ccagcactcc ggctccgctg
    12661 cagtacctcg ccccgtactc cggctgcacc atgggagagt ttttccgcga ttcagggcgg
    12721 gacgcactga tcatctatga cgatctgacg aagcatgcat gggcgtaccg tcaggtgtcc
    12781 ctgttgatgc gccgcccgcc aggccgcgaa gcctatccgg gtgatgtgtt ttatctccat
    12841 tcccgattgc tggaacgtgc ggcgaaactc tccgatgacc tgggtggcgg gtcgcttacc
    12901 gcactaccga tcatcgagac tcaggaaggc gacatctcca cgtacatccc gacaaacgtg
    12961 atttccatca ccgacgggca gattttcctc gaaagcgatc ttttttacgg cgggcagcgc
    13021 ccggcgatca acccggggat ttcggtgtcg cgcgtcggta gcaaggcgca gattcgtgcc
    13081 atggcagata gagacgtggc gggacgtctt cgcacagacc tggcgcggta caatgaagtc
    13141 gcggcgttcg ctcagttcgg gtctgatctc gacgcggtca cgcaacagac gctgaggcgt
    13201 ggtgagcggc tgcaggagat tctgaaacag ggtcagtacg taccgatgcc tgtcgaagag
    13261 caggttcttt cgcttttcgc cgctactacg ggagcactgg acgaactgcc tgtgcattcg
    13321 gtgagacagt gggagcgcga atacctggaa aacatgcgag ccgcacacgc ggacgttctg
    13381 aaggaaatca gggaaaagcg aacaatcccg gcacctttga aggaaaagat ccgggaaatt
    13441 acgaagcagt tcacaaagac gttcaaaccg gcgggaaccg cgtgaaacgg tcagcagacc
    13501 cgcgataagt atcggataga gaggtcccca tgcccaatct gcggatgata cggcgtcgaa
    13561 tcaacgccgt gaacaacatc cagcaggtca cccgcgcgat gaagatgatc gcggcctccc
    13621 gcatgcggaa ggcgcaggag aacattctcg tggcgcgccc ttatgtgcgc cgcctggaga
    13681 ctctgcttcg ccacatagcc tcccgtattc ctcagggagc gcacccgctt ctggacgtga
    13741 gaccggtcaa gaaggcgctg ctgattgttg tcgcaggcga tcgcggtctt tgccgggggt
    13801 tcaacgcaaa cgccattcgt cgtacggtgg agcgctacgg ggagctgaaa gcgcagggtg
    13861 tggagtgttc gttgatcgcg gtcggacgaa agccctatga atacttcggg aaacgcggtt
    13921 tcccgatagc cgcctcacat acaggaatct tccaacacct gaatttcctt gacgcgcagg
    13981 aaatcgcgtc tgaagcgaca aagctcttcc tgaaccgtga cgcagacctc gtggagatag
    14041 tgtacaacga gttcaaatcg gtgatccagc aggtggtggt tctcgagcgg tttcttccgg
    14101 tggagcccat cgccccggag gacggggata tgccatgcca ctacctgtat gagccctcac
    14161 tggaagcaat ctggagttcc attgtcggga aattcgtcaa tctggaaatc tggcgtgtgt
    14221 tgcttgagtc gaatgcggcg gagcagtcgg cccggatgac cgcgatggaa gaggcgacga
    14281 aaagcgcgga agagatgacg aaacgactga tcggtatcag aaatcaggtc cgacagacca
    14341 acattacgac ggagttgact gaaattgtcg gtgcggccaa cgcactggag taacgtgttt
    14401 gagaatgttg tgcaggcacg tgggtgccgt gccgaacatc cggcagggaa acgttttcac
    14461 aagctacggg aaacccgctg atgaatgtag gtaaagtagt tcaagtcatg ggcgcggtca
    14521 tagacgttga cttcactggc gctgaattgc ccgcgatcct gaatgcgctc gaaatcgata
    14581 gagaaaatgg cgagcggctg gttctggagg ttgcgcagca cctcggagag aacaaggtaa
    14641 ggacgatcgc catggacgcg accgagggac tggtgcgcga tatgcccgtg cgggatacgg
    14701 gtgtcccgat ttctgttccg gtcggcaaga gaacgttggg gaggatgctg aacgttatcg
    14761 gcgagcccat cgacggaaaa ggtgaccttg gaacggggaa acgtctgccg attcaccggc
    14821 cagcgccact cctcaccagc ctgacggtca aagccgaaat gttcgagact ggcatcaagg
    14881 tgatagacct tgtgcagccg atcatgcgcg gaggcaaggt cgggttgttc ggcggcgcag
    14941 gtgttggcaa gacagttctg attacagagc tcatccacaa catcgccaag gagcatggtg
    15001 gatactcggt ttttgccggg gttggcgaac ggacccgaga gggcaacgac ctgttccttg
    15061 agatggggga atccggggta ctggagcgca ctgctctggt tttcggccag atgaacgaac
    15121 cgcccggggc tcgtgctcgc gtcgcattga cgggactcac ccttgcggaa gacctgcggg
    15181 atgagtccgg tgtggacgtc ttgttgttca ttgacaatat cttccgtttt gtgcaggcga
    15241 actcagaagt gtcggctctt ctgggcagga tgccctccgc cgtggggtac caaccgactc
    15301 tgggaacgga catcggagaa ctccaggaac gcatcacctc aacaaaacag ggctccatca
    15361 cgtcgatgca ggcgatctac gttcccgccg atgactatac ggatcccgca ccggtggcca
    15421 ccttcgccca tctcgacgcg gtaacctacc ttgaccgtgc gatcgtggaa cagggccgct
    15481 atccggccgt tcatccgcta cggtcaacgt cgcgaattct ggacccgctc gtcgtcggcg
    15541 aggaccatta caacgttgct cgccaggtgc aacaggttct tcagcgctac gaagaccttc
    15601 gcgacatcat cgctatcctg ggcatggatg agctgggcga cgacgacaaa atggcagttc
    15661 ttcgcgcgcg gaaaattcaa atgtttctta cgcaaccgat gtttgttgcc gaggcgttca
    15721 cgggtacccc gggaaaatac gttccagtgg tggaaacggt gcggggatgc cgtgagatac
    15781 ttgagggcag acacgatgat cttcccgaag aggccttccg catggtagga acgatagatg
    15841 aggccgtgga acgagctgaa cggctcagga gtggttcata gtgccgggaa cgttttccct
    15901 cgaaatcgtc acccctacgc ggattctctt tcagggcgaa gtggaaatgc ttcgcgcgcc
    15961 gggaactctg gggtcgttcc aggtttttcc gggacatgct cccctgattt ccactcttga
    16021 ggtcggcgag attgacatcc gtctgcccgg tggcaaggaa cgagccattg ctacttcggg
    16081 agggtttgtc gaagtccttc ggaagggtgt aacggttctt gctgaaaccg cggaattcgc
    16141 cgaggagatc gacaaagccc gcgcagaaca ggccgttgcc cgcgcgaaac agcaacttga
    16201 gcatctgcgc acgaccgcac gcgtgaaggc cgaagcggcg acacgccagg tgagcgacgc
    16261 ggaagtggaa atggcgctca tcagggcgtt gaaccggttg cgtgttgcgc aacgggaaca
    16321 ctgatacgaa gggcgctctg gagaggagaa cagagtgcat catccccgcc tccgatgatc
    16381 ctcgcgcaca aggcagtttc gcagtgacag cactccccag agacccgaca cacaacgggc
    16441 gaaggccgca cccccatggc tcctgacatg aacgagatct ccgatgagca aacgggcgcg
    16501 cgcgttgtgc gtctcacccc gccggcaggc ctccactatc accagtactg ttatggccag
    16561 tggatcgctc cggactgcaa gacgcttctc gtatttcgct accgggagct tcggcgcgat
    16621 tccccgatag acctgtaccg cgtcaatact gatgggaagg gatacgcgct tgttgcggag
    16681 aacgccggct ggtccaccat ctcggtggac gggcagttcg tttttgccgg taccgcaggc
    16741 ggcattctgc gcattcctct ttccggcggc gaatctgaca cagtgtttcg ctcagattcc
    16801 taccgtggaa tgtcggcgaa cgcacagacg cgggatgggc gatttttgtt ctgtcacgca
    16861 ttcggcgaac ggggacacga actggttcgt gtgcaactcg acacggggga ggcgaaggtt
    16921 cttttccaga cggattacct catgcacctt cacctgcacg acgaaggtca gggtcgtctt
    16981 ctcgccaccg tccgtcctcc cgatttggaa tgggg
//
LOCUS       DAOTNG010000023        16903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3387_length_16903_cov_5.212132, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000023 DAOTNG010000000
VERSION     DAOTNG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_16903_cov_5.212132"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="P5179_02155"
     CDS             <1..773
                     /locus_tag="P5179_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRS94051.1"
                     /translation="IVLGSGWSGLAEMAEPIGVWDYSGIPGLPETTTPGHPGRLMYAV
                     WHGVACVVLVGRWHVYEGHTFDEAALPGMLACALGCRWLLSTNAAGGVASHLRVGDLV
                     LAEDDIFLWNAKGFRQHAGKRFSHIPRYSERLENAFDRAGSAVNVRFSKGVLALMTGP
                     CYETPAEVGMIRAAGASVVSMSTLPESRWARLAGLDAAAVSCVTNLVPSSPLARIRHD
                     EVLEVLEMSLGKAARLIERWLEDEYVRGASGRRCYGLA"
     gene            760..1623
                     /gene="accD"
                     /locus_tag="P5179_02160"
     CDS             760..1623
                     /gene="accD"
                     /locus_tag="P5179_02160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRS94052.1"
                     /translation="MGWLDAWKAGFARNAKKAIPDGVWVKCEGKGADAQLGCGKTLYR
                     RDLEENLWVCPSCGFHMRIGAHAYLDLLLDEGTAEPLFETVMSADPLQFKAEKRYGDQ
                     IMEDFRKTGRGSAFMAASGAIYGRPFAVGAMDFSFRGGSLGSAEGEKINRLIGFAQDR
                     RVPLVIVSQSGGARMQEAALSLMQMAKTSARLAEFAESSLPFISVLTSPTTGGVTASF
                     AMLGDVILAEPGALIGFAGARVRETIKEDLPPGFQRAEFLLEHGFVDRVVDRRSLKEE
                     VARLTKVLAPV"
     gene            1671..2765
                     /gene="ugpC"
                     /locus_tag="P5179_02165"
     CDS             1671..2765
                     /gene="ugpC"
                     /locus_tag="P5179_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRS94053.1"
                     /translation="MATVVLKNVTKVFDQKVFAVRDANLEVADKEFVVLVGPSGCGKT
                     TTLRMIAGLEEISSGEIYIDGRLVNDIPPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKYPRTEIDARVREAAQILEIGELLERKPKALSGGQRQRVAVGRAIVRKPRVFLFDE
                     PLSNLDAKFRVAMRTEISKLHDRLGATMIYVTHDQVEAMTMGDRIVVMDAGAIQQIDT
                     PINLYDAPRNKFVAGFIGSPAMNFVEGIIEDGTGTVFNEGSFRLPVPAHLTSALHEYS
                     GKKVVLGIRPESLLLSDDKATSGSFDARVELVEPMGSETFVYARTGNHALIARVDAHV
                     APPNGSTINLIPRPDKMHFFDAESGAAIRA"
     gene            complement(2769..3170)
                     /locus_tag="P5179_02170"
     CDS             complement(2769..3170)
                     /locus_tag="P5179_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94054.1"
                     /translation="MGFRALVGSVWCALLGISFAACGNNVAKLVSPLQTSGSVTIEIS
                     RSTTPVFSWNATTTIEAISVANFRVDGTVDRILWGYRGIAATPPITYGSVIPGGISLS
                     VGTPPALQSGNRYRVEVVSGGKASTADWIVP"
     gene            complement(3286..3723)
                     /locus_tag="P5179_02175"
     CDS             complement(3286..3723)
                     /locus_tag="P5179_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRS94055.1"
                     /translation="METIRVHSDFHAAHRQIGYVGKCRHVHGHTWRGTISLSTEKFPR
                     NHLDMAIDFGDLKQVFRRMDHKMLVGEADREFVTAQGVDPEGVVVIPGSNPSVENIAY
                     YCLEGVRKVIENQYPGRGLKYHLELTLQETENNIFVVETDFVI"
     gene            4046..4807
                     /locus_tag="P5179_02180"
     CDS             4046..4807
                     /locus_tag="P5179_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS94056.1"
                     /translation="MNQREPQEPYIRALRREIEELTKQLSDMERNHQRAIGELKASLF
                     VSQVQTVDAIVEAIEAKDPYTSGHSRRVMELSLMIGAAMHLEQERMWRVHVGALLHDV
                     GKIGVLDQSLRKTTRLDDLEYLQLKAHPLIGERIVRKIDQFADVAPIVRWHHERYDGQ
                     GYPDGLKGDQIPVESAIIFVADAYDAITSKRTYNKPRTRVEAADEIQKHAGTQFHPDV
                     AAALRAALEPNRLESIHIDESDFQDGFDIPEDIGI"
     gene            4797..5639
                     /locus_tag="P5179_02185"
     CDS             4797..5639
                     /locus_tag="P5179_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS94057.1"
                     /translation="MASELSREGFSLEMDVNKPVFGLSTAHIAHESVRQAIDTCCALE
                     LDGVEFFTREYTVAECREIRQMAQDAGLFVDYHAPWDGVYNFGQTPVDIAFDRLESAV
                     ARTHLMGGRHLVCHLGLFDLAEKDGRNRALDRVLKLTERIVPALEDTGVMLCWEDNTL
                     CHDPNPLGDQPYDFAFLFSAIQSPYVGMTLDTGHAHVTGNTRAYLEQFGNRVYYLHLN
                     DNNGIDDQHVAPSSGTIDWPELMQQICHYGAAPCFGIEFNEREVPLELPFLRSLAAPL
                     PWHW"
     gene            5760..8474
                     /locus_tag="P5179_02190"
     CDS             5760..8474
                     /locus_tag="P5179_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS94058.1"
                     /translation="MSFSTRGWLLASRSMDLLRQEIELAPRFGINHVQLSHSFCAYAE
                     QIVESSELQRDAAELIERAHRHGIEVFVWTHEMQNVPDHLKLGGKVDLNSRRTWDYVA
                     QKYEDLFSAVPELDGVVLTLTETRIRIDDDNEVVSDRPQPHRIARMVSMLDAICRKHK
                     ARLIVRTFTWVPRTMLWYAEAMRDVPDHVTIMTKESWGDWYQAQPPNPLLGLFGRHPQ
                     MIEFDCWGEYAGGTDFPWVSYEHTRNRLRYAASLGLTGAIARVDRAERSTFGSLNEFN
                     TIAFSELAKNLDKDLESLWRDWLTSKYPSDAVDAIGRALQRTNQIVQLTFYTKGILNV
                     CTSSPSLVEAEADSMIRFYRDFHGQWKTKLAVLADQLANPTEETLVQILWEKDRALSL
                     CEKSIADIESIRSKLETAKYEILHTGFEKTRVQVRAWRAMTEVVYLHKILERDPTPRL
                     REWFERAGRNLERVAEDIEHVHGKGFSRVRPEPLRNVWRVAESIHSKSTGWSTPLGLY
                     VAASPAIGDLEGNGSLSVVAVSSHKEIVALDGNGRRMWTTTTRGERYEYPHFSSPLII
                     DGRDGGGARVVVGAADGRLYCLDGQGRHVWEFATGNRVDSAPAAGDLNGDGIEEIVAA
                     SLDGTVSCLNLRGERLWNVDLLEPIFAAPVITPAREIIVVTLQGTIACLKADGEIRWR
                     QALVGGEIHRTSFQGVPSGKKYALAEGGPNAIYSSPLLCQLLGAGKPALVLGVVTGKV
                     LAFDLDGTLLWEATTAGRVFSSPAVLPGDGKKACVVIGSDDGRVHGIDCAGRLSWQFQ
                     TGGAVRSSPVVVPASIAGEARIVVGSDDNRVYVIDEEGREVDDYLTGAEIFGTPAIAD
                     VNGDGSLEIVVGSYDHRVYGFRTPWKAEKYSVISGTFRSTNTRTGCVT"
     gene            8555..9316
                     /locus_tag="P5179_02195"
     CDS             8555..9316
                     /locus_tag="P5179_02195"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRS94059.1"
                     /translation="MNAPADNACVVRVNVFLSRCGVASRRSADKLIASSRVTVNGLPA
                     ELGARIDTTRDVVCLDGRVITPVESKTTLVMNKPSGYLVSRRDPHYTRTVYDLLPSEY
                     AHLVPVGRLDLDTEGVLLMTNDGGLVEHLTHPRYRIPRVYRALVKGHPDAQALDRLRN
                     GVEIEGGTTSPAEVRVCEKRKDTSELELVLREGRKREVRLMCRAVGHEVLRLKRTEFA
                     GIGLAGLATAAWRVLSAAECERLWKQVECVDGRGD"
     gene            9300..9977
                     /gene="cmk"
                     /locus_tag="P5179_02200"
     CDS             9300..9977
                     /gene="cmk"
                     /locus_tag="P5179_02200"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRS94060.1"
                     /translation="MVVAIDGPASSGKSTTAREVAKVLGFTHIDTGAMYRALTLAALR
                     RNVRLEDDEELTRLAREVRIELGQENGVPWVKLDGEDVSQAIRTPEVDRHVSRVSEVA
                     GVREEIVRQQRVLAGSRDVVMEGRDIGTVVFPNAEVKVFLVADPVTRARRRKAELEQR
                     GIVRPLEEVLSEIVRRDEYDSSRAVSPLKRAEGAEDLDTSELSIQQQVDEVVARVRRY
                     LEKVHTT"
     gene            9974..10627
                     /locus_tag="P5179_02205"
     CDS             9974..10627
                     /locus_tag="P5179_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRS94061.1"
                     /translation="MTNQPSPYSFWSRVLYRIIWSIIRFFLVVAFRIKVSGRENERMH
                     GRVIYASNHISALDPPVVGASLKRQLMYLAKQELFAIPIFGRIIRALHARPVNRAGYT
                     RGALEVMKAALEKDEGVLVFPEGTRQKDGRLGEGKVGVGMLAVWTGAPVVPVYVSGTA
                     NVWKALSGRCRFRVALGRVVEPPDVSDPQERKRAYQTVTDAVMAEIARLKAEVDEKQ"
     gene            10740..12671
                     /gene="rpsA"
                     /locus_tag="P5179_02210"
     CDS             10740..12671
                     /gene="rpsA"
                     /locus_tag="P5179_02210"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRS94062.1"
                     /translation="MAEEQITPSAAEPAPVEQPAADKAPVQATKEERTPARVKFVSDV
                     NELPRDDEYASEEYEQLISKYESSFAVKEGEIVKGKVLRIENDEVIVDIGFKSEGSIK
                     LAEFTDPSEAQVGNEIEVFVEEIEDQEGQVVVSKQKADFMRVWDRIKEAFDSGQPVAG
                     TLTKRIKGGIVVDLFGVDAFLPGSQIDLRQVKNFEQYLGKTFDFRIIKLNKNRRNIVV
                     SRRVLLEEERETMRDELLKTLEKDQIREGLVKNITDFGAFIDLGGVDGLLHITDMSWG
                     RINHPSEVVKIGDHIRVKVLNYDKERGRISLGLKQLAQYPWKGVQERYPVNSRVKGRV
                     VSITDYGAFVELEEGVEGLIHVSEMSWTQHVRHPSKVLNIGDEVEALVLRVDEANEKI
                     SLGLKQTQPDPWETLDERYPVGTKLIGKVRNITNFGAFVEIEEGIDGLVHISDLSWTK
                     RIKHPSEVVKKGDDLPVVVLNIDKEQRRISLSHKHCEENPWDSLEAQYQPGVQTSGVI
                     SRVTDKGVVVDLEGDVEGFIPVSQLGIEDMRTPEEHFSPGETLNMVVVEFDREQKRIV
                     LSVKRYLEHQSEEELKEYLAAHVQRPITIADAVEAKGEDFPEAAEGATEEPVDAVHDD
                     EPADREDATPASSDEQSEG"
     gene            12975..13982
                     /locus_tag="P5179_02215"
     CDS             12975..13982
                     /locus_tag="P5179_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRS94063.1"
                     /translation="MAHLTVRRWWLWITVLLVAVVFVAVISVGLGSTWVPASDVLAAF
                     GSSRGVNAVIVREVRLPRVLLAGVVGGGLAVAGVVFQALLRNSLADPFVLGISGGAGL
                     GAVIAVSIGGGVSIAGLAGVPLFAFFGALIAIIVVYLFARGGGRVNLHTMLLAGVVTN
                     SVCSSAIMALTITLSYDQLQTVMFWLMGHLSTMPLPLVGKLALYVGIGLVFLMAIGRD
                     LNQMSLGESQAATLGTNVESVKRIAFVGASLVTGASVAAAGLIGFVGLIIPHAVRIST
                     GPDHRLLIPASFLAGAAFLMLADALARTVIAPTELPVGVITALIGGPVFLVLLKQRMK
                     R"
     gene            14003..15070
                     /locus_tag="P5179_02220"
     CDS             14003..15070
                     /locus_tag="P5179_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94064.1"
                     /translation="MEHSPLRVAIVGQGRSGYDIHVARLRNDSRFRIVAVADFDKARR
                     EQSARELGCKTYRTHAEMLRRTDAELVIVSSYSFSHAPIACQALEAGKHVLVEKPIAM
                     NTRWVDRMIRARDQSGKCLFPFHNYRYTKEYLHLKEMIASGILGDVFEIRMRALAFSR
                     RNDWQTTRLYGGGVLNNTCPHFLDLLLQMLQSPVAEVFTDLKLVQNVGDVENHVRVIM
                     KGRNGRIADMLVSSADAFPEPKWTILGSNGTLICDGKTSELRYFDPGDLQKGYGVRRG
                     PSPNRSYDFADSIPWKQKTVAAESHVPHDLHDNVFAVLRENAAQDITVEDVRDVVAIT
                     ERARRKDGFYGGVSRAYNPER"
     gene            15281..16885
                     /locus_tag="P5179_02225"
     CDS             15281..16885
                     /locus_tag="P5179_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94065.1"
                     /translation="MLVTHDELHDYGEITPRSVLFGAVACALISVLLPTSEYLIGASR
                     MNLSQLPVGAFGLFFTIVLVNIGIGRFFPTWALSPAEVLAVFAMALIGAVMATADLLG
                     WVFAVDAVPYYLATPENRWMDDLWPHLRQWAVVQGPSEELRWAFVGMPSGVAIPWRIW
                     VVPAFWWGTFVGAVAFGSICLASIMRRQWAEHERLAFPLAQVPLDIMGNPGGRFNIPA
                     MMRKRAFWIGASIPLFVIMWNIVYYFQPQFPRLPIMDEQGIQLGPGFPETLAIKLNMY
                     VVGFAYMVNTNVLFSVWVWYLIVFLQSTILLRVGYTLGGADDLYSSRDALTSWQGFGG
                     FIVFVFWGFWMARHHLRDVWYSVIGKIKANDEHELLPYRWAVPGLVAAALYMGGYLIA
                     LGMSWGMVLVYLFGAFVAFYGTTRVIAQTGLVYMQSPLTPTMFTFYAFGTQGVASSAI
                     VGMVGTYSLVVNGRAPLMPGIFHVSYLGAKIGRSGRRMFVALAMALTVAYLIGTIYMI
                     YISYTHGVTTFLSWAYHQHGEQGMRG"
BASE COUNT         3570 a         4423 c         5263 g         3647 t
ORIGIN      
        1 tgatcgttct gggcagcggg tggtctggcc tcgcagagat ggccgaaccg atcggggtgt
       61 gggattactc gggaataccg gggcttccgg agaccacaac tccggggcac ccggggcggc
      121 tgatgtacgc ggtgtggcat ggcgttgcct gcgtggtgct ggttggccgc tggcatgtat
      181 acgaaggcca cacgttcgat gaagctgcgt tgcccggcat gcttgcgtgc gcgctcgggt
      241 gccgctggtt gttgagcacg aacgccgccg gtggtgtggc aagccatctt cgtgttggcg
      301 acctcgtcct cgcggaggac gatatttttc tctggaacgc gaaagggttc aggcaacacg
      361 cggggaagcg tttctctcac atcccacggt attctgaacg tttggagaac gcgttcgacc
      421 gagctggaag cgcggtgaac gttcgatttt ccaagggtgt gcttgccttg atgaccgggc
      481 cgtgctacga aacgccggcg gaagtaggca tgatacgagc tgcaggggcg tccgtggtca
      541 gcatgtcaac gcttccggaa tcgcggtggg cacgccttgc gggtctcgac gccgcagcag
      601 tttcgtgtgt gacgaatctc gttccttcca gtccgctggc gcgaattcgg cacgatgagg
      661 tgctcgaggt gcttgaaatg tctctgggga aagctgcgcg cctgattgaa agatggcttg
      721 aggacgagta cgtgcgaggc gcttctggaa ggagatgcta tgggttggct tgacgcgtgg
      781 aaagctggct ttgccaggaa cgcgaagaaa gcgattccag acggtgtgtg ggtcaagtgc
      841 gaaggcaaag gcgccgacgc tcaacttggt tgcggtaaga cattgtacag gcgcgacctg
      901 gaagaaaacc tgtgggtgtg cccatcgtgt ggctttcaca tgcgaatcgg ggcgcacgca
      961 taccttgatc tgctcctgga cgaagggacg gctgagccct tgttcgaaac cgtaatgtcg
     1021 gccgacccgc tgcaattcaa agcggagaaa cggtacggcg accagatcat ggaggacttc
     1081 cgcaaaacgg gtcgcggcag cgcattcatg gcggcgtccg gggctatcta cggcaggcct
     1141 tttgcggttg gcgcgatgga cttctcgttt cggggcggga gtctcggttc agcggaaggc
     1201 gaaaagatca accgcctcat cggtttcgca caggaccgga gagtacccct cgtgatcgta
     1261 agccaatccg ggggtgcgag gatgcaggaa gcggcgctat ctctcatgca gatggcgaaa
     1321 acgagcgcac gattggccga gtttgcggaa tcatcgcttc ccttcatcag cgttctgaca
     1381 agcccgacca caggcggggt tacggccagc tttgccatgc tcggagacgt cattcttgcc
     1441 gaaccgggag cattgatcgg gttcgccgga gcgcgtgtcc gggaaacgat taaggaggac
     1501 ctgccgccgg gtttccagcg cgcggagttc ttgcttgagc atggattcgt cgaccgcgtt
     1561 gttgatcgcc gtagtctcaa ggaggaggtc gcgaggttga cgaaggttct tgctcccgtt
     1621 tgaacgtcgg gggctgccgg cgcaactgcc acaggagagc gagtctgaag atggcaacgg
     1681 tagtgctgaa gaacgtcaca aaggtattcg atcagaaggt tttcgcagtt cgggacgcga
     1741 accttgaggt tgctgacaag gagttcgtcg ttcttgtcgg cccttccggc tgcgggaaaa
     1801 ctacgacgtt gaggatgatc gcggggttgg aggaaatcag ctcgggggaa atctatatcg
     1861 acggtcggtt ggttaacgac ataccaccga aggaccggga catcgcgatg gtgttccaga
     1921 attacgcgct ttacccacat atgaccgtgt acgaaaacat ggcctttggt ctgaagctcc
     1981 ggaagtatcc ccggacagaa atagatgcgc gcgtcagaga agctgcccag attcttgaaa
     2041 ttggggagct tctggagagg aaaccgaaag cactctccgg tgggcagcgc cagagggttg
     2101 cagtgggacg ggcgattgtc cgaaagccca gggtgtttct tttcgatgaa ccgttgtcga
     2161 acctggatgc gaaatttcgc gtggcgatgc gcaccgagat atccaagttg cacgacaggc
     2221 tgggcgcgac gatgatctac gtcacgcacg accaggtgga agcgatgacg atgggagaca
     2281 ggatcgtcgt tatggatgcg ggcgctattc agcagatcga tacgccgatc aatctttacg
     2341 acgctcctag gaacaagttt gtcgcaggat tcataggttc gcctgccatg aattttgttg
     2401 aggggattat cgaggatgga actggaacgg tgttcaatga agggagcttc cgactaccag
     2461 tgcctgcaca cctgacatcc gcgcttcacg agtattcagg gaagaaggtg gtgctgggaa
     2521 tacgaccaga atcgttgctt ctcagcgacg acaaggcgac atcgggctca tttgatgcca
     2581 gggttgaact cgtggaaccc atggggagtg aaacgtttgt gtacgcacgg actggaaacc
     2641 atgccctgat cgcgcgtgtg gacgctcatg tggctccgcc gaacggttct acaatcaacc
     2701 tgatcccgcg ccctgataaa atgcactttt tcgacgcaga atccggcgct gccattcgcg
     2761 cataaccctc agggcacgat ccaatcggca gtgctcgctt tgccgccgct gaccacctcg
     2821 acgcggtacc gatttccact ctgaagcgca ggtggtgttc cgacggagag acttattcct
     2881 ccggggatta cgcttccgta ggtgattggt ggagtggcgg cgatcccgcg gtatccccac
     2941 agtatcctgt ccacagttcc atcaacgcgg aaattcgcga cactgatggc ttcgatggta
     3001 gttgttgcgt tccacgaaaa aacgggagtt gtagagcgag agatctcgat cgtgacactt
     3061 ccgctcgtct gaagcggact caccagtttg gcgacgttgt tgccgcacgc ggcgaacgat
     3121 attccgagaa gcgcacacca gactgatcct accaaagccc ggaaacccac tccgccgacc
     3181 tcccttttcc accacaaatg tgcaggcaca gtttgtgcgc gcgcaacctc gccgaccgac
     3241 tgaagaaatt agccaacccg ggcggcttct cccaatacct gttcctcaga tcacgaaatc
     3301 agtttccact acgaagatgt tgttttccgt ttcctgaagt gtcagttcca ggtggtattt
     3361 gagtcctctg cccgggtact ggttctcgat caccttcctg acgccttcca gacagtaata
     3421 tgcgatgttt tccacgctgg gattggaacc cggaatcacc acaacgcctt ccgggtctac
     3481 tccttgagcc gtgacaaatt ctctgtcggc ttctcccacc agcatcttgt ggtccatccg
     3541 gcggaacacc tgtttgaggt ctccgaaatc tattgccatg tcaaggtggt tccttgggaa
     3601 cttctccgtg ctcaacgaga tcgtccctcg ccatgtgtga ccgtgcacgt gccggcattt
     3661 gccgacatac ccgatttgcc tgtgagccgc gtggaaatcg ctgtggacgc gaatagtttc
     3721 cattgttccg ttccctccct gtctatgttc ttcgttaata gtacggatta cccgtccggg
     3781 gagttccccg gcgcgcggga tgtgggatca tcggcagccc tgcgagaacc ctgcaaaaag
     3841 agcgctggcg ggcgtgacga ggttcgttca ggcgttggag cgggcgcgca acggggttaa
     3901 tttcgaaaga actgcatgaa acgggaaaac gcgcgcttct gtgttttcgt accggagacg
     3961 tgagcggtgt tcgctgagtg acgtgcaacg aaggttgctg gcgagggctc ccgttccgga
     4021 cgtagggatg gatacgacct ctaagatgaa ccaacgagag cctcaggaac cgtacatacg
     4081 agcgttgcgt cgcgagattg aagaacttac gaagcagctc tcggatatgg aaaggaatca
     4141 ccagcgggcg atcggggaac tgaaagccag cctctttgtc tcacaggtgc agacggtaga
     4201 tgcgattgtt gaggcgattg aagccaagga tccctacacc agcgggcatt cccgtcgcgt
     4261 aatggaactg agtcttatga taggcgcggc gatgcatctt gaacaggaaa ggatgtggcg
     4321 ggtccacgtg ggtgcattgc tccatgatgt cggtaaaata ggcgttctcg atcagtcgtt
     4381 gcgcaaaact acgcgtctcg atgaccttga gtacctgcag ctgaaagcgc atccgctgat
     4441 tggagaacgg atcgtgcgca aaatagacca gttcgcagac gtggcgccga tagtccgctg
     4501 gcaccacgaa cgttacgacg ggcaaggata tcccgacggc ctcaaaggcg atcagatacc
     4561 ggtggagtcg gccataattt tcgttgctga cgcgtacgac gcaatcacga gcaaacgaac
     4621 gtacaacaag ccacgaacca gagttgaggc ggcagacgaa attcagaaac acgcgggtac
     4681 ccagtttcat ccggacgttg cagccgctct gagggcggcc ctcgaaccca atcgactgga
     4741 atccatccac atcgacgaat cggatttcca ggacggattc gacatccccg aggatattgg
     4801 catctgaact gagtcgggaa ggcttttcgc tggaaatgga cgtgaacaaa ccggtttttg
     4861 gactcagcac agcgcatatt gctcacgaat ccgtgagaca ggcgatcgac acttgttgtg
     4921 cccttgaact cgacggcgtg gagtttttca cccgggagta cacggttgcc gaatgccggg
     4981 aaatccgcca aatggcacag gatgcaggtt tgttcgtcga ttaccacgct ccgtgggacg
     5041 gagtctacaa cttcggccag acccccgtcg acatcgcatt tgatcggctg gaaagtgcag
     5101 tggcgcgcac acatctcatg ggcgggcgcc acctcgtatg ccaccttggt ttgttcgatc
     5161 tcgcggaaaa agacgggaga aaccgcgcac tcgatcgggt actgaagctg acggaaagga
     5221 tcgttcccgc gctcgaggac actggagtca tgctctgttg ggaggacaac acgctgtgcc
     5281 acgaccccaa ccctcttggc gatcaaccgt acgacttcgc gttcctgttc agcgcaatcc
     5341 agtcgccgta cgtcggtatg acacttgata cggggcatgc gcacgtgacc ggcaatacgc
     5401 gcgcctacct tgagcaattc ggaaatcgtg tgtattacct tcacttgaat gacaataacg
     5461 gcattgatga ccaacacgtt gcgccgtcgt cgggaaccat tgactggccc gagctgatgc
     5521 agcagatctg ccattacggt gccgcaccct gtttcggtat cgaattcaac gaacgagaag
     5581 tccctctcga actgcctttc ctccgatctc tggcggcacc actgccgtgg cactggtgac
     5641 ctccgcaatt ccccttcatt ttcgcttctg cttctgtatc ttagacgtga gaacacactg
     5701 gaagcatgtg tgatcacgcc ggacgggttg ttgtgcacct gcgcggggag ggctctgcga
     5761 tgtctttctc tacgagagga tggctccttg caagccgcag catggacctg ctgagacagg
     5821 agatcgagct tgcgcctcgg ttcggaatca accacgtaca actgtcgcac tctttctgcg
     5881 catacgccga gcagatcgtc gaatcctcgg agcttcagcg tgatgccgca gaactcatcg
     5941 agcgggctca ccggcacggt atcgaggtgt tcgtctggac gcacgaaatg cagaacgtgc
     6001 ccgaccatct caagctgggt ggcaaggttg acctgaattc gcgccgaacg tgggattacg
     6061 ttgcccagaa atacgaggat ttgttctccg cggttcctga acttgatggg gtggtcctca
     6121 cactcacgga gacccgaatc cgcatagatg acgacaatga ggtggtaagc gaccgaccgc
     6181 aaccgcacag gatcgcgcgg atggtaagca tgctcgatgc gatatgccga aaacacaagg
     6241 cgcggctcat cgtcaggacg ttcacctggg tgccgaggac gatgctctgg tatgccgaag
     6301 cgatgcgcga cgttccggat cacgtgacga tcatgacgaa agagtcgtgg ggagactggt
     6361 atcaggcgca gccaccgaat ccactcctgg gactttttgg tcgccatcca cagatgatag
     6421 agttcgattg ctggggggaa tacgccgggg ggacagattt cccgtgggtg agttacgagc
     6481 acacacgcaa tcgtctgaga tacgccgcat cacttggcct cacgggagct attgcgcggg
     6541 tggaccgtgc agagcgcagt acgttcgggt ccctgaatga gttcaatacg attgcgttct
     6601 ccgaactggc aaagaacctg gacaaggacc tggaaagtct ctggcgcgat tggctgacat
     6661 cgaaataccc atccgacgcc gtggatgcca tcgggcgggc gctccagagg acgaatcaga
     6721 ttgttcagct cacgttctac acgaaaggca tactgaacgt gtgtacgagc tctccgtccc
     6781 tcgtggaggc agaagctgat tccatgattc gtttctaccg cgatttccac gggcaatgga
     6841 agacgaagct cgccgttctg gcagaccaat tggcgaatcc aacagaggaa acgttggtcc
     6901 agattctgtg ggaaaaggac cgggcgctga gcctctgtga aaagagtatc gcggatatcg
     6961 aatccatcag aagcaaactc gaaactgcga agtacgagat actccacacc ggtttcgaga
     7021 aaacgcgggt tcaggttcga gcgtggcggg cgatgacgga ggttgtctac ctgcacaaaa
     7081 tcctggaacg tgaccccacc ccccggctga gagaatggtt tgaaagagcg gggaggaatc
     7141 tcgaacgggt tgccgaagac attgagcatg tccatgggaa gggtttttcg cgagtgcgtc
     7201 cggaaccgct tcgcaatgtg tggagggtag cggaaagcat ccacagcaag tcaactggct
     7261 ggtcgacacc tctcgggttg tacgttgctg cgtcacccgc aatcggggac ttggagggca
     7321 atgggtccct ctcggtggtt gcggtgtctt cccacaaaga aatcgtggcg ctcgacggca
     7381 acgggaggag aatgtggaca accacgacga ggggcgaacg atacgaatat ccgcacttct
     7441 cctcacccct gattattgat ggccgagacg gtggcggtgc cagggtagtt gtgggcgctg
     7501 ccgacggcag gctttattgc ctcgacgggc aggggcggca cgtatgggaa ttcgcgacag
     7561 gtaatcgtgt ggattccgca cccgctgccg gcgacctgaa tggcgacgga attgaggaga
     7621 tcgtggcagc gtcgctggat gggacggtgt cctgcctcaa tctgcgcggc gaacggctgt
     7681 ggaatgtcga tctcctggag ccgatttttg cggcgccggt gattactccc gcgcgggaaa
     7741 tcatcgtggt gacgctgcag ggtacgattg cgtgtctgaa agcggacggg gaaatccggt
     7801 ggcggcaggc tcttgtcggg ggagagattc atcggacttc gttccaaggt gtgccttcag
     7861 gaaagaaata cgcgctcgcg gaaggaggtc cgaatgcgat ctactcgtcc cctcttcttt
     7921 gtcaactcct cggagcggga aagccggctc tcgttctcgg ggttgttacg gggaaggtgc
     7981 tcgcgttcga cctcgacgga acgcttctgt gggaagccac taccgccggg cgagtgtttt
     8041 cttcacccgc tgtgctgcca ggtgacggaa aaaaagcctg cgtggtgatc ggctcggatg
     8101 acggacgtgt acacggcatc gattgtgccg gccggctgtc atggcagttc cagacgggag
     8161 gtgcggttcg ctcttctcca gtggtcgtcc ccgccagtat cgcaggagag gcgcggatcg
     8221 tcgtaggatc agacgacaac cgtgtttacg tcatcgacga agaaggccgg gaggtggatg
     8281 attatctcac aggagcggag atttttggca ctcccgccat tgctgatgtc aacggagatg
     8341 ggagtctgga gattgtggtc ggatcgtacg atcatagagt ctacgggttt cgtacgccgt
     8401 ggaaggccga gaagtacagt gtgatcagcg gaacattccg ctcaacgaac acccgtaccg
     8461 ggtgtgtcac ctagagtcac cgccggcccg ttacttgacg ccgaccccat attgagcgta
     8521 aaattaaaag attctgtgaa aacaagaggt tcctgtgaat gcgccagccg acaatgcttg
     8581 cgttgttcgg gtgaacgtgt ttctttcgcg gtgcggtgtc gcttcgcgaa gaagtgccga
     8641 caagctcatc gcttcttcgc gcgttaccgt gaacggtctg ccggcggaac tgggcgctcg
     8701 gatcgatacg acccgggatg tcgtttgtct tgatggtcgc gtgattactc ccgtcgagtc
     8761 aaaaacgact ctcgtgatga acaaaccttc cggctacctt gtctcgcgga gggatcctca
     8821 ctacaccaga acggtgtacg acctgttgcc gagcgagtac gcgcatctgg tccccgtcgg
     8881 aaggttggac ctcgacaccg aaggtgtgtt gctgatgacc aacgatgggg gactggtcga
     8941 gcaccttacg catccgcgtt atcgcatacc acgcgtgtat cgcgcgctgg tgaaggggca
     9001 tcccgatgct caagcattag atcgccttcg caacggcgtg gaaatagaag gtggtacgac
     9061 gagccccgcc gaagtgaggg tgtgcgagaa gcgcaaggat acctctgagc tggagttggt
     9121 tcttcgagag ggcagaaaac gggaggtgcg cctcatgtgc cgcgcagtgg gacatgaggt
     9181 tcttcgactc aaaagaaccg aattcgccgg aatcggtctc gccggccttg ccacggcggc
     9241 gtggcgagtg ctctcggcag ccgaatgcga acggctgtgg aaacaggtgg agtgcgtgga
     9301 tggtcgtggc gattgacggt cctgcaagct cggggaagtc cacgacagcg cgtgaagtag
     9361 cgaaggttct gggtttcact catatcgaca ctggcgcgat gtaccgtgcg ctgacactgg
     9421 cggctctgcg tcgaaacgtt cgcctcgagg acgacgagga attgacgaga ctggcccggg
     9481 aggttcggat agaactcggg caggaaaacg gcgttccgtg ggtgaaactg gacggggagg
     9541 acgtaagtca ggcgatccgc acgccggaag tggatcggca tgtgtcgcgc gtgagcgagg
     9601 ttgcgggggt tcgcgaagag atcgttcgcc agcaacgcgt tctcgcaggc tctcgtgacg
     9661 tagtgatgga aggccgtgat attggtacgg tggtttttcc gaacgcggaa gtgaaagtct
     9721 tcctcgtcgc tgatccggtc accagggcgc ggagacgaaa agcggaactg gaacagcgag
     9781 gaattgtaag acccctggaa gaggttcttt ccgaaatcgt caggcgggat gagtatgatt
     9841 cgagtcgcgc agtttctccg ctgaaacgcg ccgagggcgc cgaggatctg gacacttctg
     9901 agttgtccat tcaacagcag gttgacgaag tagttgcacg agtgcgcaga tatctggaaa
     9961 aggtgcacac aacgtgacga accagccgtc gccgtactct ttctggtcac gcgtgcttta
    10021 tcggatcata tggtcaatca tcaggttttt cctggtggtg gcatttcgta tcaaggtatc
    10081 agggcgtgaa aacgagcgga tgcacggacg cgtgatatat gcgtccaatc acatttccgc
    10141 gttggatcct cccgttgtgg gagcatcgtt aaaacgccag ctcatgtatc tcgcgaaaca
    10201 ggagcttttc gcgatcccga tttttggccg catcatccgt gccctccatg cgcgaccggt
    10261 gaaccgcgcc gggtacactc gaggcgcgct ggaggtgatg aaagcggccc ttgaaaagga
    10321 tgaaggagtg cttgtgttcc ccgaagggac ccgtcagaaa gacggcagac tcggggaagg
    10381 caaagtgggt gtcgggatgc tcgcggtttg gacgggggca ccggttgttc cggtgtacgt
    10441 cagtggcacc gcgaatgtct ggaaggcgct ttcgggccgt tgccgtttcc gcgttgcgct
    10501 ggggcgcgtc gtggaaccgc ctgacgtctc ggatccgcag gaacgcaaac gggcttatca
    10561 gacggtgacg gatgccgtga tggcggaaat cgctcgcctg aaagccgagg tagacgagaa
    10621 gcagtaacca ccattcttcc acgtcatcag caacgttctt cagttgcgat gacgtgccgc
    10681 gcagacagga cgaccgtgct cgcgccaccg ggatacgagc gtccgggaag gaaaccacaa
    10741 tggcagaaga acagatcacc ccgagtgcag cggaacctgc gcccgtggaa caacccgccg
    10801 cggacaaggc gcccgttcag gcaacgaaag aagagcgaac acccgccagg gtcaagttcg
    10861 tttctgacgt gaacgagctc ccccgggacg acgagtatgc ttccgaggag tatgaacagc
    10921 tcatcagcaa gtacgagtcg tcgttcgctg tcaaagaagg cgaaatcgta aagggtaaag
    10981 tcctgcggat cgagaacgat gaagtcatcg tggacatcgg tttcaaatcc gagggatcaa
    11041 taaagctcgc cgaattcacc gacccgtcgg aagcacaggt cgggaacgag atcgaggttt
    11101 ttgttgagga aatcgaggac caggaaggcc aggtcgtggt ttccaaacag aaggccgatt
    11161 tcatgcgggt atgggaccgg atcaaagaag cctttgactc aggccaaccg gttgctggga
    11221 cgcttacgaa acggattaag ggtggcatcg tcgtggatct gttcggtgtt gacgccttcc
    11281 tgccgggctc gcagattgac ctgcgtcagg taaagaactt cgaacaatat ctgggcaaga
    11341 cgttcgactt ccgtatcata aagcttaaca agaaccggcg gaacattgtt gtcagccggc
    11401 gtgtgcttct cgaggaagag cgcgagacaa tgcgtgacga gttgctgaaa actctcgaga
    11461 aggatcagat acgcgaagga ttggtcaaga atatcaccga tttcggtgct ttcatagatc
    11521 tcggcggcgt ggacggtctt ctacatatca ccgacatgtc ctgggggcgg ataaaccacc
    11581 cgtcggaagt ggtgaagata ggcgatcaca tccgggtgaa ggtgctcaat tacgacaagg
    11641 aacgcggtcg gatttccctc gggttgaagc aacttgcgca gtacccatgg aagggtgtgc
    11701 aggaacgata tccggtgaac agccgcgtga aggggcgcgt cgtatccatc accgattacg
    11761 gagcctttgt cgaacttgaa gagggcgttg agggcctgat ccacgtatcg gagatgtcgt
    11821 ggacgcagca cgttcgccat ccttcgaagg ttctcaatat tggcgatgaa gtagaagctc
    11881 tcgtgctgcg cgttgacgaa gcgaatgaga agatcagcct cgggctcaaa cagacgcagc
    11941 ccgatccgtg ggaaacgctg gatgagcgct atccagtggg gaccaagttg attggcaaag
    12001 tccgaaacat caccaacttc ggggcgtttg tcgaaatcga agaaggcatt gacgggttgg
    12061 tccatatatc ggatctctca tggaccaaac ggatcaagca cccgtcagag gtcgtcaaga
    12121 agggcgatga cctgcccgtg gtggttctca atatcgacaa agagcaacgc aggatttccc
    12181 tttcccacaa gcactgtgaa gagaacccgt gggatagcct tgaggctcag tatcagcccg
    12241 gcgtccagac ctccggagtc ataagccgtg taacggacaa aggcgtggtg gttgatctcg
    12301 agggcgacgt ggaaggattt attccggtga gtcagctcgg catcgaggac atgaggactc
    12361 ctgaagagca cttcagcccc ggagagactc tcaacatggt ggtcgtggag ttcgatcgcg
    12421 aacagaaacg aatcgtgttg agtgtgaaac ggtacctcga gcaccaatca gaagaggaac
    12481 tgaaggagta cctcgcggct catgtgcaac gcccgatcac aattgcggac gcagtggaag
    12541 cgaagggaga ggatttccct gaagctgccg agggtgctac ggaagagcct gtggatgctg
    12601 ttcacgatga cgaaccggca gacagggaag atgcaacgcc agcctcgtcg gatgaacaat
    12661 ccgaagggta gcaggaacaa gtggtttgtc acgaacgcgt gtgccatgag gtcagggggt
    12721 gcttcgaaca ccccctgacc atttgtgccc tgtggctggt atagctgcga tattgcgcat
    12781 atatctgggg agtgtcgcaa accgggagca tttctccccg gaccgcgagt ctgtttgccg
    12841 aaaatagaag tcgttcttcc caggaatttt gatacaaccc gcaagggccc gggtccactt
    12901 ccgaacaagg gatccaaggt cccggcagaa gtgtttgcgt cagcctgacc tcagtctatt
    12961 tggaggtacc tgaattggct cacctgacgg tgcgtcgctg gtggctctgg attaccgttc
    13021 ttctggttgc cgtggtgttc gtcgcagtga tttcggtggg cctggggtct acatgggttc
    13081 ccgcgagcga cgttctggca gcgttcggct cttcgagagg cgtcaacgcc gttatcgtgc
    13141 gggaagtccg gcttccccgt gtgctgcttg ccggggtggt tggaggaggc cttgcggttg
    13201 ccggtgtcgt gtttcaggcg cttcttcgga actcacttgc cgatccgttc gtgctgggca
    13261 tatcgggcgg cgcaggactg ggcgcggtga tcgcggtatc catcgggggt ggcgtgagta
    13321 tcgccgggtt ggccggtgtg ccgctgttcg ccttctttgg tgcgctgatc gcgatcatcg
    13381 tggtctacct gttcgcccgg ggcggtggcc gggttaacct ccatacgatg ctgctggccg
    13441 gggtggtcac aaattccgtc tgttcttctg ccatcatggc gctgacgata actctatcgt
    13501 acgaccagtt gcagacagtg atgttctggt tgatggggca tctgagcacg atgccgcttc
    13561 cgctcgtagg aaaacttgct ctttacgtgg gaatcgggct cgtttttctc atggcaatcg
    13621 ggcgcgattt gaatcagatg agtctgggtg agtcacaggc tgccacgctc gggacaaacg
    13681 tggaatcggt gaaacggatc gcgtttgtcg gcgcctcact ggtgacgggt gcttcggtgg
    13741 cggcagcggg gttaatcggg tttgtcggtt tgattattcc acacgcggta cggatttcga
    13801 ccggcccgga tcatcgtctg ctcattccgg ccagttttct cgcaggtgct gcgtttctga
    13861 tgctggcaga cgcgctggcg cggaccgtga tagcaccgac ggagttgcct gtgggcgtta
    13921 ttaccgcgct gatcggcggc cccgtatttc tcgtactcct caagcaacgg atgaaacggt
    13981 aactatcgga agggaagagg aaatggaaca ctcgcctctg cgcgtcgcca tagtgggtca
    14041 agggcgttcg ggttacgaca ttcacgtcgc tcgattgagg aacgattcaa ggttcagaat
    14101 tgtcgcggtt gcggacttcg acaaagctcg tcgggaacaa agcgcacgag aactcggatg
    14161 caaaacatac cgcactcacg ccgaaatgtt gcgacgaacc gacgccgagt tggtcattgt
    14221 gtcttcttac tcgttttcac acgctccgat cgcatgccag gcactcgagg cgggcaaaca
    14281 cgtgttggtg gaaaagccga ttgcaatgaa tacccgctgg gtggacagga tgattcgcgc
    14341 gcgggatcaa tccggcaagt gtctgttccc tttccacaac taccgttaca cgaaggaata
    14401 ccttcatctg aaggagatga ttgcttccgg catactgggg gatgtgttcg agattcggat
    14461 gcgcgctctg gctttctcgc gccgaaacga ctggcagacg accagactgt acggcggagg
    14521 ggtactcaac aacacgtgcc cgcatttcct cgaccttctt ctgcaaatgc tgcaatcccc
    14581 ggtggcagag gtgtttacgg acctcaaact cgtccagaat gtcggagacg tcgagaacca
    14641 cgtcagggtc atcatgaaag gcagaaatgg gcgtatcgcg gacatgctcg tttcttccgc
    14701 ggacgccttt cctgagccca aatggaccat actcggaagc aatggcaccc tgatctgcga
    14761 cggaaaaaca agcgaactcc gttacttcga ccccggggac ttgcagaaag ggtacggcgt
    14821 caggcgaggg ccctccccca accgcagtta cgatttcgcg gactcaattc cgtggaagca
    14881 gaaaactgtc gcggccgaat cacacgttcc ccacgatctc catgacaatg tgtttgctgt
    14941 tctgcgggaa aacgccgcac aggatatcac ggtcgaggac gtccgcgacg tggtagcgat
    15001 caccgagcga gcgaggcgga aggacgggtt ctatggcgga gtatcccggg cgtacaaccc
    15061 cgagcgttaa ctctcttccc gaagccggaa ttgcccgcca atccgaggaa cggagcgact
    15121 cgttgcggca aatccgctgc gcaggactcg acccgtgtga tgttgttggg taactttcat
    15181 ttgtcgtaaa tgggaaagcc gttgataatc cgcgctttcc cggcgccagt tcctgcaacc
    15241 gcgcgctggc gccttctttc attttccttc ggtacgttct atgctcgtta cgcacgatga
    15301 actgcacgat tacggagaaa tcacgccccg gtcggtgttg tttggcgccg tggcatgcgc
    15361 gttgataagc gttcttcttc ccacgtcaga gtacttgatc ggcgcttccc gcatgaacct
    15421 gagccaactt cccgttggcg cgttcggtct ttttttcacg attgttctgg tgaatatcgg
    15481 gatagggcgc ttctttccga catgggctct gtcaccggcc gaggttctcg cggttttcgc
    15541 gatggcgttg atcggggccg tgatggcgac ggccgatctt ctcgggtggg tgtttgcggt
    15601 agatgcagtt ccgtactacc tcgccacgcc ggaaaatcgg tggatggatg atctctggcc
    15661 gcatctcagg caatgggcgg tggttcaggg gcccagcgag gaactacggt gggccttcgt
    15721 gggaatgccc agcggagtag ccattccctg gcgcatctgg gtggttccgg cattctggtg
    15781 gggcactttc gtcggtgccg ttgcattcgg gtcaatttgt cttgcctcca tcatgcgcag
    15841 gcaatgggcc gaacacgagc gtcttgcgtt tccactcgcg caggtgcccc tcgacatcat
    15901 ggggaacccg ggcgggcggt tcaacatacc ggcgatgatg aggaaacgcg cgttctggat
    15961 aggggcgagc atcccgctgt tcgtgatcat gtggaacatc gtctactact tccagccgca
    16021 gtttccccgc ctgccaatca tggatgagca aggcattcaa cttggcccgg gcttccctga
    16081 aaccttggcg atcaaactga acatgtacgt cgttggtttt gcgtacatgg taaacacgaa
    16141 cgttctgttc agcgtctggg tatggtatct catcgtgttc ctccagagca cgattttgct
    16201 tcgagtcgga tacacgttag ggggtgcgga cgatctctat tcatcgaggg acgcgctgac
    16261 gagctggcag ggttttggtg gattcattgt cttcgtgttc tggggattct ggatggcgcg
    16321 ccaccatctg cgcgatgtct ggtactccgt tatcggcaag atcaaggcga acgatgagca
    16381 cgagctgctt ccgtaccgct gggctgtgcc gggcctggtc gcggccgcgt tgtacatggg
    16441 aggatacctg atcgccctcg gcatgtcgtg gggtatggtg ctggtctatc tgttcggtgc
    16501 gttcgtcgcg ttctacggaa cgacgcgcgt aattgcccag accggactgg tatacatgca
    16561 aagccctctc acgccgacga tgttcacgtt ctatgctttt ggaacgcagg gggttgcgtc
    16621 ctcagcgatc gtcgggatgg tcggcacata ttcgctcgtt gtgaatgggc gtgcgccact
    16681 tatgccaggg atcttccatg tgagttatct tggcgcgaag atcgggcgca gtggccggcg
    16741 aatgtttgtg gcgcttgcga tggcgcttac cgttgcctac ctgatcggta cgatctacat
    16801 gatttacatc agctacacgc acggcgtgac aacgttcctc tcctgggcgt accaccagca
    16861 cggagagcag ggaatgagag gatagtgcgg cgcctagggc cat
//
LOCUS       DAOTNG010000024        16758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3426_length_16758_cov_4.716877, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000024 DAOTNG010000000
VERSION     DAOTNG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_16758_cov_4.716877"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..637)
                     /locus_tag="P5179_02230"
     CDS             complement(47..637)
                     /locus_tag="P5179_02230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRS94066.1"
                     /translation="MSLVIFVVFGCVAVGCALAMVFARNPVYGAIALIVCLAALAGEY
                     LLLDAPLLAAFQILVYAGAIMVLYLFVIMLLNVRREEGCRWCTDWRTYLGFVLAGLIG
                     LSFLFKASNVAHPAPPVGFHVYDVTGVAYTMFTDPTLLFIIEALGVLLLMSVVGAIYL
                     GRRFSAEEELLMKHDQPAGSSGTGAAGDAPCGETTP"
     gene            complement(660..1277)
                     /locus_tag="P5179_02235"
     CDS             complement(660..1277)
                     /locus_tag="P5179_02235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRS94067.1"
                     /translation="MATINVQRPERLTLAERLYLPAILNGLRLTWRHIWGERVTLQYP
                     EEEREFKRVYRGVHRLNKDDKGRVKCVACEMCAASCPAHCITIKATEVEWPERDKIPK
                     IFEIDELKCIFCGYCVEACPEDAIHMTRHYHLIARSREDMIFGLDKLLAIYDETKEQE
                     VLRGVDFGVIRARIPGKEAGNPDEVQKLAYPVVGVVTKAETHHAQ"
     gene            complement(1330..2388)
                     /gene="nuoH"
                     /locus_tag="P5179_02240"
     CDS             complement(1330..2388)
                     /gene="nuoH"
                     /locus_tag="P5179_02240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRS94068.1"
                     /translation="MISQEQLIVSLALGLVLMLAMTAGAGVFTFFERKIAGWTQGRYG
                     PNRAGPKGYLQFLADGIKFALKEDVIQDYVVKPVYVIAPGLMMIPALVLYAALPIGKP
                     FVWNGNEVHLQVVQANVGVLYLLAVASIAVYGIVFGAWASNSRYPFFGGVRASAQMIS
                     YELAMGLSIISVVLLTSEPGINALSISNIVAGQTGYWFGFIPRWNLFANPVAFVVFLV
                     CMFAETNRLPFDLAEAEQELVGGYHTEYSAMKFASFMLSEYVNMITMSMVMVTLFVGG
                     WHFPGIEAIGSQVIQVLLSIGAFGIKVLFFCFLFLWVRWTLPRFRYDQLMHLGWKALL
                     PVALASALGTAIAIALRV"
     gene            complement(2385..3866)
                     /locus_tag="P5179_02245"
     CDS             complement(2385..3866)
                     /locus_tag="P5179_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRS94069.1"
                     /translation="MPTMVIDGREVTAPPGANLIEAAKLAGIEIPHFCYHPALKVVAS
                     CRMCFVEVTQEVRGKATTRLTTACNTPAADGMVVRTDTQPVKKAQNDILEFLLINHPV
                     DCPVCDEAGECKLQDYAFAYGTASSRFAEHKRFRHAKKLGSGIRLYTNRCILCDRCVR
                     FLRDFVGTGELSVTDMGNGNEISVFPGKPIDNVLAGNLPEICPVGALISEDFLFKARV
                     WNLSAVDSVCPGCSRGCATSLDVKDNEVQRTRARENQAVNGYFICDDGRYLYHVIRHE
                     TRLEHPAVREGDQLRESNWDHALDVVVSILAEFPERVVILLSPFLTQEEARAGADLSD
                     VLRGSVLGHVPNAVQEAPVLFPGGFAIDGDKAPNSTLVKNIAPVPLQELAITKDTVVL
                     FFNSSLRTGSVSPLDAEKLRRAGFVAVIDVLRNDITAVADVVLPGRMWAEKSGTFSNS
                     AGMAQSFGAAVLPPPFTRDESDVIGDLTRRITTRISTGCGAKS"
     gene            complement(3955..5307)
                     /gene="nuoF"
                     /locus_tag="P5179_02250"
     CDS             complement(3955..5307)
                     /gene="nuoF"
                     /locus_tag="P5179_02250"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRS94070.1"
                     /translation="MAARQNRMILDQCEIPGIRNLAVARRYGAYTALAKALTTMTPDQ
                     VVEEITRSGLQGRGGAWFGTGLKWSFMPKEPRVESFLCVNADESEPGTCKDRYLIEKN
                     PHLLLEGVLIACYAIRASTAFIYVRGEMWEGMEVLETAIRELVTANLCGKNILGTGFT
                     INVHVHPGAGAYICGEETALMESLEGKRGYPRNKPPFPAAYGAFGHPTTINNIGTLGY
                     VPWIINHGADAFKGIGDPNFPGTLIYQVSGHVNRPGLYEAELTVTLRDLIDMAGGVRS
                     GHRLKAVIPGGTSMPLLTADDLDTVMSPASFAPRGSLKSGLGSAAVIVLDDTCDMVDV
                     ALNMMRFYRHESCGQCTPCREGTKWVCDMIERIHRRRARPGDLEVLEQVLVNVGDIDR
                     FEFNKTICLFGVSFGWPLTMLIRRFRSEFEAAVAESTPAPVPLPVPASVLPAGDLRAP
                     "
     gene            5602..>7573
                     /locus_tag="P5179_02255"
     CDS             5602..>7573
                     /locus_tag="P5179_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94071.1"
                     /translation="MSDHFARIQSLGNHDHDDVSLRSIVLGTLACIVVVVWAPLSEFL
                     VGASRLNLSQLPVVAMGLFFGVVLLNSVVGRIAPVLRLRPAELITVFSMAFVGSIVAT
                     SNLLNWPLGIMSVPYYVATPENRWMSDLWPYLRQWAVVQGPRDELRWAFVGMPDGAKI
                     PWGIWIVPMFWWGTFLGAVALVAICLASLLRKQWADNERLAFPLVQVPLELITSPSGK
                     WGISETLRKRSFWVGAAIPLFVILFNCLNYFLPQFPRLPVMDGISIQLGPNFPAFGIK
                     LNMYVLGFAYLVNTNVLFSVWFWDIILVLESGFFRSVGYTLGDADDPYSSRDALTSWQ
                     GFGGFIVFVLVSLWMARNHLKMVWYAVIGKIKADDENELLSYRWAVIGLFVAVVYMTG
                     FMVSLGMSWKMALVFLFGAFVAYLGTTRVIAQTGLVYMQSPLTPTMFTFGAFGTVGVP
                     AQEIVGMVGTYSLVVNGRAPFMPAIFHISWLGAKIGARGRRMLTVLIVALIVAYACGS
                     FYYLMIAYNEGATTFRSTHFAFHGEQVYNMIIQKMQARVAVDPGRWTFLGAGALVMSI
                     LTYLQYRFPAWPLHPIGFPIAAANNVHRTVFAIFLAWLIKTIILKIGGVEAYERGRPV
                     FIGMAAGYALGILLSFFVDWMWFPGAGHQIHNW"
     assembly_gap    7574..7673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7674..8126)
                     /gene="nadD"
                     /locus_tag="P5179_02260"
     CDS             complement(<7674..8126)
                     /gene="nadD"
                     /locus_tag="P5179_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRS94072.1"
                     /translation="MSEPNDVDTPSRNRRVGLLGGTFDPPHNGHLALALYAQEHVGLD
                     QIWFIPSAVPPHKMQKTSTPAALRWEMTCAAVNPYSPRLVVSDVELKRPGPSYTYQTV
                     QRIRRMLGNEAELFWIIGRDNIAEIPLWMEPERIVEEATVIAGGRPGVP"
     gene            complement(8137..8910)
                     /gene="bamD"
                     /locus_tag="P5179_02265"
     CDS             complement(8137..8910)
                     /gene="bamD"
                     /locus_tag="P5179_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRS94073.1"
                     /translation="MLLRRAPIRLLVCITVLSVISACGPKRTHLLTCEEYYQRGINAL
                     EKKKWLKAQENFEKITMNFPGCDLVDDAQYMLGETYFRQDKFIEAQFEFRRVVEDFRQ
                     SDRTEDAQFKMALCSHRQALPAALDQTATQDAIYRFQQYLEDNPSGRWAGEARRYILE
                     NRKKLAQKDFNTAQFYARQGYNEGALIYLDHVIAEYPDTGEWLERARFLKATILAQRG
                     QTQEALTLLRAINLDALPGKVQKKVRETTARIESPQENR"
     gene            complement(9031..10284)
                     /locus_tag="P5179_02270"
     CDS             complement(9031..10284)
                     /locus_tag="P5179_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94074.1"
                     /translation="MRYRLLVPHLLVFAAACHASSIFSYHPRGLGYAEWKGDARTMGM
                     GGVSLANSGEDGATSANPAGIAGCERTALSASFLSQKRSVKDYLGYAGTFYDQYPRFF
                     RAVVPIGRGIVSSFGLEPVGDVRMIWANRNTENGVSYLDSLEASGGAWSFGAQVARSF
                     GPISLGLKAQVVRGNMTTEWRRTVVDSAAPLATSALAMRRISGAAFSLGSAYRVSEEW
                     AIGGRLDFPSNLSETDITSYGTRIPNQYYKSHTDVVMAVASDLDDTTSSTAKLPVGFG
                     AGVAWRPGERLLVSLDGEFRRWSDVDPAFRNTWCVAGGVEFRVSDDYRSFLLWQLPYR
                     AGVRWEQHYVPALSSHGGATYPDGKYLTLGAGIPLGRNRGRLDYSVEYSTRGTISTNL
                     VREKLWRHTISVVGWERWFERSTRR"
     gene            complement(10281..11630)
                     /locus_tag="P5179_02275"
     CDS             complement(10281..11630)
                     /locus_tag="P5179_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94075.1"
                     /translation="MKFLRSQSFPVLTILLAAVASLLFEACSSTKSAVGGGLLTDVTG
                     EFSRRDTIDIPLSGVSPATVACFPVHISFSSSEALLAGEANGLRATVLLKFSPIVSWT
                     EERYYTLNDQAALDTLTFTPVSVVTDSSSQLRLTYYGHVADSSFCAVQTRLVVSSWKE
                     EDSVMTALPAMNAPPFSPVRVDTTASGSVVYLPFAPASLTQLIADSVSLAVETTSGSS
                     MVRFLSQEGATTYSPQLTLWIKANVTSKRPEIAAHDTLIGLTLRPIHDCYRLQRLVPA
                     PAPSAGSLRISAGDAWRGYVRFDRFPIPGVSDSAGVVRARATANSAQLQLILGPDGGG
                     SFKRDSAAILIYPLTKPFDPDSARAGLLNFGTGRLYGVGFNTAETRAAPDVRVFEIAD
                     LINQWWVNPSSNNGLLISLSGEAQLVDALEIRSMRLIVTTTMPPQLIGKPASGEAPR"
     gene            complement(11809..12780)
                     /locus_tag="P5179_02280"
     CDS             complement(11809..12780)
                     /locus_tag="P5179_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94076.1"
                     /translation="MAVRIGFIGCGGIANHHMRTMAGNPDAEMVAFSDPDVERARAAA
                     KQYGGKAYSSYRTMISKGGIDAVFMCVPPFAHTDQEKLVAEAGLAFFTEKPIGLSLKR
                     VAENAELFAKKGIITAVGYNWRYSDGAAMMKKTLDGKKLAVVSGYWVGGTPGVMWWRQ
                     MKKSGGQVVEQTTHIFDMCRYVAGEIDVVFASGISGLTSQDELLLYDVHDASVVQLRY
                     RSGAVGNITSSCIVSQGVGTQVRAYARNLVVVVGEGRCQVSIPGEEHIYKDARNPTAH
                     EQEAFLCAVAKKNGPPILSDYADAMKTLRVTLAANKSMETGKPVKLA"
     gene            complement(12814..13605)
                     /locus_tag="P5179_02285"
     CDS             complement(12814..13605)
                     /locus_tag="P5179_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS94077.1"
                     /translation="MKLACQEGLVPGKSIAEKLANLQKAGYEAMEVWGGGLPDRVKEI
                     RAACKSSPVKISSVCAGYGGCPLDSDPAGRQKASDDIKRLLDATGEIGAVGLIFVPIF
                     GGPRIGDLSPWKTAVELEKDLLVELLSGWADHAAAAKTLVILEPLNGYETHLIKKLAD
                     GVDICERVNKPNGLKMMADFFHMGLEERDVAASIKAAGKWLCHVHLSDHPRILPGYGH
                     SDFKKGFAALKSIGYTGFMALECGCPDADKMKAITKTAKFLKAQM"
     gene            complement(13619..14659)
                     /locus_tag="P5179_02290"
     CDS             complement(13619..14659)
                     /locus_tag="P5179_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94078.1"
                     /translation="MPVVFGIVGCRHGHVFSLIKELLSAPNTECAGVFDSDQDSLRAV
                     TSRFPVRAAASETELLDNPDVTLIGTAEINRKKAPLILNALNAGKHVIADKPLCTSLH
                     ELNAIERTATEKRLKIGLMLTERFGPANRRMKQLVSDGFVGHVANVMCWRPHRLGRAT
                     RPDWMFVDDLYGGILVDLAVHDADIIRWLTGGNFLEVTAYQYNMGNPADTDFSDIGVM
                     LGRLSTGATGFVRTDWFTPATSPVHGDTRFLVTGTTGMIEVRTSGDLWGGEAGKEPQV
                     LFISDTQPATRFDGIGPERPLVHDFLRSITDGRPAEITTADVFEATRTTLMARLSCKL
                     GRTVTRSESYLA"
     gene            14850..16640
                     /locus_tag="P5179_02295"
     CDS             14850..16640
                     /locus_tag="P5179_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS94079.1"
                     /translation="MKPRISHSIPHLSAALALVFFGCSPVEKTPPAQNPAELPSPAGV
                     ETPADSEYGGRATKALAEGEGIRGTARTSSTADISATESRLRGLSVDGAQAAQVKAGT
                     HDDNEEYPSWVMFSSRKWLLPMVLPAAYHGRTVLRVIDSAGAPVMAQSYTVKDAGNAP
                     LWRGVTYANGETVLFPSVFWSDRATPASVVCHLSNGQEIQAPWRPSLDGFVTVQLDAP
                     RRTPRKIPVDVAFVLDATGSMGDEIQQLRDVLFSIHSRLQNAARAADIRFGLVVYRDR
                     GDAEPLRVVPFTANVDSFQLALESVSASGGGDTPEDVHAGLRATLKDLAWREGSIRNA
                     FLIADAPPHTDYGQEYDYLWASRTANSMGIRINTVGASGLDQSGEHAFRQISVATYGQ
                     FIFLTYGETGESSGMGSAADPGRVSHHTGANWSARRLDDIVVDLVRRDIAYQTEVPRL
                     VTDSPPPVDQHDHLAVRMENLWQQVQRQLAAFSGDTLTAVLLPFESTLDDSSHLEAYL
                     RDVSTETLLRLGSVRMVERDRLAQIIAEQSLSVSAAIEPSRAIELGHLLNSRVVFLGK
                     IYRLGTDRVVHVRAVETETGQIVAAARVRV"
BASE COUNT         3445 a         4982 c         4715 g         3516 t
ORIGIN      
        1 atgacgaaaa gaatcgtgct gagccagaga acgtgtatgg tctggatcat ggggtggttt
       61 ctccgcaggg ggcgtctccg gccgctcctg tgccgctcga tcctgccggt tggtcatgtt
      121 tcatcagcaa ctcctcctcg gccgagaacc ttctcccgag gtagattgcg ccgacgactg
      181 acatcaggag cagcaccccc agcgcctcga tgatgaacaa aagcgtcggg tcagtgaaca
      241 tggtgtatgc cacgcctgtg acatcataca cgtggaaacc aaccggtgga gcagggtgcg
      301 cgacgttcga ggccttgaaa agaaaagaga gcccgataag ccccgcgagc acgaaaccaa
      361 gatacgtccg ccagtcggta caccaccggc atccttcctc cctccgcaca ttcaggagca
      421 tgatcacgaa caaatagaga accatgatcg cgcccgcgta aacgaggatc tggaacgccg
      481 cgagaagagg ggcgtcaagg agtaggtact caccggcgag tgccgcaaga cacacaatga
      541 gggcaattgc gccgtagacg gggttacggg cgaataccat cgccaacgcg caaccgactg
      601 ctacacagcc aaacactacg aaaatcacca gcgacatatg ccccgccttc tttgtccctt
      661 cactgcgcgt ggtgtgtctc cgctttcgtg actacgccga ccacaggata ggcgagcttc
      721 tgcacctcgt cgggattgcc cgcttccttt ccaggtatgc gggcacgtat cacgccaaaa
      781 tccactccac gcagaacttc ctgttccttc gtctcgtcgt agatcgcaag cagcttatca
      841 aggccgaaaa tcatgtcttc gcgtgaccgc gcaatgaggt gatagtgcct cgtcatgtga
      901 atcgcatcct cggggcacgc ttcaacgcag tatccgcaga aaatgcattt gagttcatct
      961 atctcaaata ttttcggtat cttgtccctt tcaggccact ccacttccgt ggctttgatc
     1021 gtgatacaat gtgccgggca agaagccgcg cacatctcgc acgcaacgca tttcacgcgc
     1081 cctttgtcat ccttgtttag tctgtgaaca ccccggtaca ctcgtttgaa ttcccgttct
     1141 tcctccggat actgcagagt gacgcgctcg ccccagatat gtcgccaggt aaggcggagt
     1201 ccgttcagaa tggcgggcaa atacagacgc tccgccagag tcaaccgctc gggccgttgt
     1261 acattgatcg tggccatctg attgcctcgc cgctgctgtt ttcttcctgt gggccggctg
     1321 cccgggctat cataccctca gagcgattgc gattgccgtt ccgagggcgg atgcaagcgc
     1381 cacgggcaga agtgctttcc acccgaggtg cataagctga tcgtaccgga atctcgggag
     1441 agtccaacgg acccagagga acaggaaaca gaagaacagc accttgatac caaaggctcc
     1501 gatgctcaac aacacctgta ttacctgtga tccgattgcc tcaatgcccg gaaaatgcca
     1561 ccctccgacg aacagcgtta ccatcaccat cgacatggtg atcatgttca cgtattccga
     1621 aagcatgaac gacgcgaatt tcatcgcgct gtactcggtg tggtagccac caacgagttc
     1681 ctgttccgct tcagcgaggt cgaatgggag gcggttcgtt tccgcgaaca tgcacacaag
     1741 gaacacgaca aacgcaacgg ggtttgcaaa cagattccaa cgcgggatga atccgaacca
     1801 gtaacccgtc tgccccgcca cgatgttgga gatgctcaac gcgttgatgc cgggttcgct
     1861 cgtcagaaga accacgctga tgatagacaa tcccatggcg agctcgtaac tgatcatttg
     1921 agccgacgcc cggactccgc cgaaaaacgg gtacctgctg ttcgacgccc aggcaccgaa
     1981 gactataccg tacactgcga tgctggcgac cgcaagcaaa taaaggacac caacgttcgc
     2041 ctgaacgact tgaaggtgca cttcattgcc gttccacaca aagggcttac cgattgggag
     2101 ggccgcatac aggaccagcg ccgggatcat catcagccct ggcgcgatca cgtacacagg
     2161 tttgacgacg tagtcttgaa tcacgtcctc tttcagcgca aatttgatcc cgtcggcgag
     2221 gaactgcagg taccctttcg gaccagctct gttcggcccg tagcggccct gcgtccaccc
     2281 ggcgattttg cgctcaaaga acgtaaatac ccctgccccg gccgtcatcg cgagcatcag
     2341 caccagcccg agagcaagcg aaacgatgag ctgttcctga gaaatcacga tttcgctccg
     2401 caccctgttg aaatgcgtgt cgtgattcgc cgtgtcaaat ctccgatgac atcagattcg
     2461 tcgcgcgtaa aagggggagg cagtaccgcc gccccgaaag actgagccat accggctgag
     2521 ttggaaaagg tgccagattt ctccgcccac atcctgccgg gcagtacaac atcagcgacc
     2581 gcggtgatgt cattccgcag gacgtcaatc accgcaacaa accctgcgcg ccgcagtttt
     2641 tcggcgtcca gaggtgacac tgacccggtg cgaagggagg aattgaaaaa gagcactacg
     2701 gtgtctttgg ttatcgcgag ttcctgcagc ggaacaggcg caatgttttt caccagtgtt
     2761 gaattcggag ccttgtctcc atcgattgcg aacccaccgg ggaacaaaac aggcgcttcc
     2821 tgaacagcgt ttggaacgtg ccccagcacg gaaccgcgca aaacatcaga aagatcagcg
     2881 ccggcgcggg cttcttcctg ggtgaggaaa ggagaaagca gtatcacgac tcgctcaggg
     2941 aactccgcca gtatcgagac aaccacatcc agcgcgtgat cccagttgct ctcccgcaac
     3001 tggtctcctt cacgcaccgc cgggtgttcc agacgcgtct cgtgacggat cacgtggtac
     3061 aggtagcgtc cgtcgtcaca gatgaagtac ccgttcaccg cctggttctc ccgtgcccgg
     3121 gtgcgttgaa cttcgttgtc ctttacgtcc agagacgtcg cgcaaccgcg ggaacagccg
     3181 gggcaaacag aatcaactgc gctaaggttc cacacgcggg ccttgaacag aaaatcctcg
     3241 gagatgagcg cccccacagg gcagatctcc ggcagattgc ccgcaagaac gttgtcgatc
     3301 ggtttaccgg gaaacacgga gatctcgttt ccgtttccca tgtcggttac cgagagctcg
     3361 cctgttccta caaaatccct caggaaacga acgcatctgt cgcaaagaat gcagcgattt
     3421 gtgtagagcc ggataccgga acccagtttt ttggcatggc ggaagcgctt gtgctccgca
     3481 aacctgctgg aggctgttcc ataggcaaac gcataatcct gaagtttgca ttcacccgcc
     3541 tcgtcgcata cggggcaatc cacagggtgg ttgatcagca ggaattccag aatgtcgttc
     3601 tgagcctttt tcacaggttg agtatcagtt cgaacgacca ttccgtctgc ggcaggcgtg
     3661 ttgcacgcgg ttgtgagccg agttgtcgct ttgccgcgca cctcttgcgt cacctccacg
     3721 aaacacatcc ggcaactcgc gacaactttc aaagccggat ggtaacagaa atgcgggatc
     3781 tcaatcccgg ccagcttcgc agcttctatg agattggcac ctggcggggc tgtcacttcc
     3841 cgaccgtcaa tcaccatcgt cggcattcag aacctctgat ctgctgctgg cagactacga
     3901 cttcccatac acaatttctg ttgcgcccgg agcaccagcc ttttgcggag ttggtcacgg
     3961 agcccgcagg tccccggccg gcaagacact cgctggaacg ggcaacggca caggggctgg
     4021 cgtgctctcc gcaactgccg cttcgaattc gcttcggaat cttcggatca gcatggtgag
     4081 gggccatcca aaggacaccc cgaacaggca gatcgtcttg ttgaactcga accgatctat
     4141 gtctccgacg ttcaccagaa cctgctccaa cacctccaga tcaccggggc gcgcacgacg
     4201 gcgatggatt ctttcgatca tgtcgcacac ccatttcgtt ccttcacggc agggcgtgca
     4261 ttgtccgcag gattcgtggc ggtagaatcg catcatgttc agagcaacat caaccatgtc
     4321 gcacgtatcg tccagcacaa tcaccgcggc cgaccccagg ccacttttca atgatccccg
     4381 cggcgcgaaa ctggccggcg acatcaccgt gtccagatcg tccgcggtga gcaatggcat
     4441 ggaggtgccg cccggaatca ctgctttgag ccggtgtccg gagcgaactc cgccggccat
     4501 gtcgatcaga tcgcgcagcg ttaccgtcag ttcggcctcg tacaaaccgg gccgattgac
     4561 atgcccgctg acctggtaga taagagttcc agggaaattg gggtcgccaa tgcctttgaa
     4621 cgcgtcggct ccgtgattaa ttatccacgg tacgtatccc agggttccga tgttgttgat
     4681 agtcgtggga tggccgaacg caccataggc ggcggggaac ggtggtttgt ttctcgggta
     4741 gcctcgtttt ccttccagcg attccatcag cgcggtttcc tcgccgcaga tgtatgctcc
     4801 agcgccggga tgcacgtgca cattgatggt gaagccggtg cccagaatgt tcttaccgca
     4861 gaggttcgcg gttaccagtt ctcggatcgc ggtttcaagg acttccattc cctcccacat
     4921 ttcacctcgg acatagatga atgcggtcga cgctcgtatc gcgtaacagg cgatcagcac
     4981 accttcaagc aggaggtgtg ggttcttctc aatgagataa cggtctttgc acgtgccggg
     5041 ctcgctctcg tcagcgttga cgcagagaaa gctctccacg cgcggttctt ttggcatgaa
     5101 tgaccatttc agaccggttc cgaaccacgc tcctccgcgg ccctgcagcc cggatcgtgt
     5161 gatctcctcc accacctggt caggcgtcat ggtagtaagt gctttggcca gcgcggtgta
     5221 cgcgccataa cggcgcgcaa cggcgaggtt ccgaatacca gggatttcgc actggtcgag
     5281 aatcatccgg ttctggcgcg ctgccatgcg gtttgctccg acggccggga aacccccgcc
     5341 tgatcattga acgagcttga tcacgtgtgc ttccacacag gaaggttaaa gataatacgc
     5401 gtgcgggtga tgaagacaag tagccgcaat cagtgagtgg gtggctgccg gggccctgaa
     5461 ggttggttcg tgcaattgac cacaaatacg tgacttggcg gggcattatt cgtatacttt
     5521 ttcttatccg acatccgaat ggtgagcaat ccgcctcgca tcgccctctt atctcaacaa
     5581 tctgatctgg aacagatcga catgtctgac cattttgcgc gcattcagag tcttgggaat
     5641 catgaccacg acgacgtctc cctgcggtca atcgtcctgg gtaccctcgc atgtatcgta
     5701 gtagttgtct gggcgccatt gtccgagttt ctggttggtg catctcgcct caatctctcg
     5761 caacttcccg tcgtcgcgat gggcttgttc ttcggggtcg tgctcctcaa ctccgtggtc
     5821 ggtcgcatag cccctgtgct gaggcttcgt cccgcggaac tcatcaccgt gttctcaatg
     5881 gcgtttgtcg gatcgattgt cgccacgtca aacctgctca actggccact gggaatcatg
     5941 tcggtgccgt actacgtggc aacaccggag aaccggtgga tgagcgatct ctggccgtat
     6001 ctcaggcagt gggcagttgt gcaggggcca cgggacgaac tccgctgggc atttgtcggt
     6061 atgccagatg gcgcgaagat cccgtggggt atctggatcg taccgatgtt ctggtgggga
     6121 acctttttag gcgcagttgc actcgtcgcg atttgccttg cttcgctgct acgaaagcaa
     6181 tgggcggata acgaacgact cgcgttcccg cttgttcagg tacctctgga actcatcaca
     6241 agtccatccg gcaaatgggg catctcggaa accctgcgaa agaggtcctt ctgggttgga
     6301 gcggcaattc cgttatttgt gatcctcttt aactgtctga actacttcct tccgcagttc
     6361 cctcgtctgc ctgtgatgga cggcatcagc attcagctcg ggccgaattt ccccgcgttc
     6421 ggcatcaaac tgaacatgta cgttctcggc ttcgcgtatc tcgtcaacac caacgtgctt
     6481 ttcagcgtct ggttctggga catcatactc gttctggaaa gcggcttttt ccgttctgtc
     6541 ggatacactc tgggggacgc agacgacccg tattcctcgc gtgacgcgct gacgagctgg
     6601 caaggcttcg gcggcttcat tgtttttgtg cttgtcagcc tgtggatggc ccgcaaccac
     6661 ttgaaaatgg tgtggtacgc agttatcggc aaaatcaaag ctgatgatga gaacgagctg
     6721 ctgagttatc ggtgggcggt gatcgggctc ttcgtggcag ttgtctacat gacgggtttc
     6781 atggtgtcgc tcggaatgtc ctggaaaatg gcgctggtgt ttcttttcgg ggcattcgtg
     6841 gcgtatctgg gcaccacacg ggtgatcgcg cagacgggcc ttgtgtacat gcagagcccg
     6901 ctcacgccta cgatgttcac gtttggcgcg ttcgggacag tgggtgtccc ggcccaggaa
     6961 atcgtaggaa tggtaggcac gtattccctc gtagtgaatg gtcgtgcccc gttcatgcct
     7021 gcgattttcc acatcagttg gctcggagcg aaaatcggtg ccaggggccg caggatgctg
     7081 acggttctca ttgtcgcgct catcgtggcg tacgcgtgtg gctcgttcta ctacctgatg
     7141 atcgcgtaca acgaaggagc aacgaccttc cggtccacgc attttgcgtt tcatggtgag
     7201 caggtgtaca acatgatcat ccagaaaatg caggcgcgtg tggcggttga cccggggcgc
     7261 tggacgttcc tcggtgccgg cgcactcgtg atgtcgattc tgacctacct gcaataccgg
     7321 tttcccgcgt ggccgctgca cccgattgga ttccctattg ccgcggcgaa caacgttcac
     7381 cgtaccgtat tcgccatatt cctcgcgtgg ctcataaaaa ccatcatcct gaagatcgga
     7441 ggcgttgagg catacgaacg cggcaggccc gtctttatcg ggatggcggc gggatacgcc
     7501 ctggggatat tgctgagttt cttcgtggat tggatgtggt ttcccggggc gggccaccag
     7561 atacacaact ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggcacg
     7681 ccggggcgcc ctcctgcaat gactgtcgct tcttcgacga ttctctccgg ttccatccag
     7741 agaggaatct ccgcaatgtt gtcgcgcccg atgatccaga acagctcagc ttcgttcccc
     7801 agcattctcc ggatacgttg gacagtctga tacgtgtacg agggcccagg acgtttcaac
     7861 tccacatccg aaaccaccag tcgcgggctg tacgggttga cggcagcaca cgtcatctcc
     7921 caccgcaagg cagcgggagt gcttgttttc tgcatcttgt gaggcggcac ggcggacggg
     7981 atgaaccaga tctgatcgag gcccacgtgt tcctgcgcgt acaacgcaag agcgagatgg
     8041 ccgttgtggg gaggatcaaa ggttccgccg aggagaccga cgcggcggtt acgggacggt
     8101 gtatcaacgt cgttcggttc gctcatatgg cagacgctac cggttttctt gggggctttc
     8161 gatgcgtgcg gttgtctcgc gaaccttttt ctgaactttt ccggggagcg catcgaggtt
     8221 gatggcacgc aacagggtga gcgcctcctg cgtctgcccg cgttgcgcga ggatggtagc
     8281 tttcaaaaac ctcgctcgtt ccagccattc tccggtgtcc gggtactccg cgatgacgtg
     8341 gtcgaggtaa atcagcgcgc cctcattgta cccctgccgg gcgtaaaact gcgcggtgtt
     8401 gaaatccttc tgcgcaagct ttttcctgtt ttccaggatg tagcggcgcg cttcacctgc
     8461 ccagcgccct gaaggattgt cctcgaggta ctgctggaaa cggtaaatgg catcctgtgt
     8521 cgcggtctga tccagtgccg ccggaagcgc ctgccgatgc gagcaaaggg ccatcttgaa
     8581 ctgcgcgtcc tccgttcggt ccgattgccg gaaatcctca accacgcggc ggaactcaaa
     8641 ctgcgcttcg atgaacttgt cctgccggaa atacgtctct cccagcatgt actgcgcgtc
     8701 atccaccagg tcacatccgg ggaagttcat ggtgattttc tcgaagttct cctgagcctt
     8761 gagccatttc ttcttttcca gagcattgat gccgcgttgg taatactcct cgcaggtcag
     8821 cagatgagtc ctcttcggcc cgcacgcgga tatgacggaa agcaccgtga tgcaaaccag
     8881 aagacgtatc ggggcgcgcc tcaataacat ggtcttcatt tcgcgaaggg aaattgggaa
     8941 agtgccatcg acgcgacacc actggcacga cgtcgggact cctttttgca gtagagtccc
     9001 gtggcaatgc gcatcccggg tccgcgccct ctaacgacgt gtgctgcgtt caaaccagcg
     9061 ctcccagccg accaccgaga tggtgtgtcg ccatagcttt tcccggacca gattggtgga
     9121 aatcgttccc cgcgtgctgt attcgacgga gtagtccaat cgtccccggt ttcggcccag
     9181 tggtatccct gcgcccagag tcaggtactt cccgtctgga tacgtggcac ctccatggga
     9241 actgagagcc ggcacataat gctgttccca ccgaactcct gcccggtaag ggagttgcca
     9301 cagcagaaac gaccggtaat cgtcgctcac gcggaattcg acgccgccgg ccacacacca
     9361 tgtgtttcgg aatgcagggt caacgtccga ccagcgccgg aactcaccgt ccaatgacac
     9421 aagcagacgc tccccgggcc tccacgccac gccggcgcca aatcccaccg gtaacttcgc
     9481 cgtggaggag gtcgtatcgt caaggtcaga cgcgaccgcc atcaccacgt ccgtatgcga
     9541 cttgtaatac tggttgggaa tgcgcgtgcc gtacgatgtg atgtctgttt cgctcagatt
     9601 cgaggggaaa tccagccttc cgccgatcgc ccactcttca gatacccgat atgcgcttcc
     9661 gagactgaac gccgctccgg agatacgccg catcgcaagt gcggaagtcg ccagtggtgc
     9721 cgcagaatcg accaccgtcc ggcgccactc tgtagtcatg ttgccgcgta caacctgcgc
     9781 tttcagccca agcgaaatcg gcccgaaact gcgggcgacc tgcgcgccaa atgaccatgc
     9841 acctccgctt gcttcaagcg aatcaaggta actcacaccg ttttctgtgt tccggttcgc
     9901 ccagatcatc cggacatccc cgacgggttc cagcccgaac gacgagacta tgccccggcc
     9961 gataggcacc acggcccgga aaaaacgtgg gtactggtcg taaaatgtgc cagcgtagcc
    10021 gaggtaatcc ttcaccgacc gcttctgact gagaaagctc gcggaaagcg cggtgcgttc
    10081 acaaccggcg atccctgcag gattcgcgct ggtagcaccg tcctctcccg aattcgcgag
    10141 agacacacca cccatcccca tggtccgcgc gtcgcccttc cactctgcat aaccgaggcc
    10201 acgggggtgg taggagaaga tcgaactggc gtgacacgcg gcagcgaaca ccagcaggtg
    10261 cggcacaagg agacggtatc tcatcggggg gcctccccgg atgccggttt cccgatcaac
    10321 tgcggtggca tggtcgtcgt tacgatcaac cgcatcgaac ggatttccag agcgtccacg
    10381 agctgggcct ctcccgaaag cgagatgaga aggccgttgt tggacgatgg gttgacccac
    10441 cactggttga tcaggtcagc aatttcaaat acccgcacgt ccggcgccgc tctcgtttcc
    10501 gccgtattga acccgacacc gtaaaggcgt cccgtcccga agttcagcag accggcgcgg
    10561 gcgctgtccg gatcgaaagg cttggtaagc ggatagatga ggatcgcggc gctgtcccgt
    10621 ttgaatgagc ctccgccgtc cggcccgagt atcagttgca gttgagctga gttcgctgtt
    10681 gcgcgagcgc gcacgactcc ggcgctgtcg ctcacccccg ggatcgggaa tctgtcgaag
    10741 cggacgtacc cgcgccacgc atcacccgcg gatatccgga gcgatcctgc ggacggcgct
    10801 ggcgccggca cgagccgctg aagtcgataa cagtcatgga taggccgcag ggtgaggcct
    10861 atgagagtgt catgagccgc gatttccggc cgcttcgaag tcacattcgc ttttatccag
    10921 agggtgagtt gcgggctgta cgttgtcgcg ccttcctgac tcaggaaccg taccatgctg
    10981 ctgccggagg tcgtttccac ggccagggat accgaatcag caatgagctg cgtcagagaa
    11041 gcgggggcga aaggcaggta cactaccgac ccggaggccg tcgtgtctac ccggacaggc
    11101 gaaaaaggcg gcgcattcat tgcggggagc gccgtcatca ccgaatcctc ttccttccac
    11161 gacgaaacca ccagccgtgt ctgtaccgcg cagaacgacg agtcagcgac gtggccataa
    11221 tacgtgagcc gcagttgact tgacgaatcg gttacgacgc tgaccggcgt gaaggtaagt
    11281 gtgtccagcg cggcctgatc gttcaacgtg taataacgtt cttccgtcca tgacacgatt
    11341 ggcgagaact tcagcagaac ggttgcccgc agtccgttcg cctctccagc gagcagtgcc
    11401 tcgctgctcg aaaaggatat gtgcaccgga aagcacgcaa cggtagccgg ggagacgcct
    11461 gaaagcggaa tgtcgattgt atcgcgtctc gaaaactcgc ccgtgacgtc cgtcaataac
    11521 ccgccaccga ccgcggactt tgtgctggaa caggcttcga acaacagcga ggcaacagcg
    11581 gcaagcagaa ttgtcagtac cggaaaggac tgcgaacgca gaaacttcat gcgcggataa
    11641 tccgttgcta gggagtagtc tcgccgatcg agaaggtgaa ccttggatac gcaggctcgc
    11701 tcgttgaagg gcggttagaa aaatagggaa acagcacgtg cgttccaatg tgcacggaga
    11761 tcacgggggt gctggtagga ccggacaccc ccgtgaacat ctgagattct atgcaagctt
    11821 gacgggtttt cccgtctcca tggacttgtt ggccgccagc gtgacgcgca gcgttttcat
    11881 cgcgtcagcg tagtcggaaa gaataggcgg cccgtttttc ttcgccaccg cgcataggaa
    11941 cgcttcctgc tcgtgagcgg tgggatttcg cgcgtctttg tagatatgct cttcgccggg
    12001 tatactgacc tggcacctgc cttctcccac cacgacgacg agattgcggg catacgcccg
    12061 tacctgtgtt cccacgcctt gtgaaacgat gcacgacgac gtaatgttcc ccaccgcgcc
    12121 ggaccggtag cgcaactgaa cgacactcgc atcgtgaacg tcgtacagca ggagctcatc
    12181 ctgactggtc aacccggaaa taccagacgc gaacaccacg tcaatttcgc ccgcgacata
    12241 ccggcacatg tcgaagatgt gggtagtttg ctccacaacc tgccccccgg acttcttcat
    12301 ctgtcgccac cacattacgc cgggggtgcc gcccacccag tacccactca ccaccgccag
    12361 cttcttgccg tcaagcgtct tcttcatcat cgcggcgccg tctgagtagc gccagttata
    12421 gcccaccgcg gtgatgatac cctttttcgc gaacagctcc gcgttctctg cgactcgctt
    12481 caacgacagt ccgattggct tctccgtgaa gaaagcgagt cctgcttctg cgaccagttt
    12541 ctcctgatcc gtgtgcgcga acggtggcac gcacatgaac acggcatcga ttccgccctt
    12601 cgaaatcatt gtgcggtaac tggagtacgc tttgccgccg tactgcttcg ctgccgcgcg
    12661 ggctcgttcg acatccggat cactgaacgc taccatctct gcatcggggt tgcccgccat
    12721 cgtccgcatg tggtggttcg caatcccgcc gcagccgata aaaccgattc gtactgccat
    12781 gacaactcct tccggtgaca atccgccgca gagttacatc tgagccttga ggaattttgc
    12841 tgtcttcgtg atggctttca tcttgtcagc gtcagggcac ccgcattcga gtgccataaa
    12901 gccggtataa ccgatgctct tcaacgctgc gaatcccttt ttgaaatcgc tgtgtccata
    12961 tcccggaaga atccgcgggt ggtccgaaag atgaacgtgg caaagccatt tcccggccgc
    13021 tttgatggaa gccgcgacgt cccgctcttc cagacccatg tggaaaaagt ctgccatcat
    13081 cttcaacccg ttgggcttgt tcacgcgttc acagatgtca accccgtcgg ccagtttctt
    13141 gatgagatgc gtttcatacc cgttcaacgg ctccagaatt acgagcgtct ttgccgctgc
    13201 ggcgtgatcc gcccacccgg aaagcagctc gaccaggaga tccttttcaa gttccaccgc
    13261 ggttttccag gggctgaggt caccgatgcg cgggccgccg aaaatcggga caaaaatcag
    13321 cccgacggcg ccaatttcgc cggtcgcgtc gagaagtctt ttgatgtcat cgcttgcctt
    13381 ctgcctcccc gcaggatcgg aatcaagtgg gcaccctccg taaccggcac atacgctcga
    13441 tatcttcacc ggagacgact tgcaggctgc ccgaatttct ttgacacggt caggaagacc
    13501 tccgccccag acctccatcg cttcgtaccc ggccttctgc agattcgcga gcttctccgc
    13561 gatgcttttt ccgggaacga gcccctcttg gcatgcgagt ttcatggcgt gctctccttc
    13621 aagcgagata cgactcagaa cgggttacgg tgcgtcccag tttgcacgat aaccgtgcca
    13681 ttaacgtggt gcgggtggct tcgaatacat ccgcggtggt tatttctgcg ggcctgccgt
    13741 cagtaatgct cctcaggaaa tcatgcacaa ggggcctttc gggtccaatt ccgtcgaaac
    13801 gcgtggcggg ctgggtgtca gatatgaaca gcacctgcgg ctcctttccg gcctctccgc
    13861 cccacagatc gccggaagtc ctcacttcta tcatgccggt ggtgccggtg accagaaatc
    13921 gggtatcccc gtgtaccggc gaggttgctg gggtgaacca gtcggtcctc acaaacccgg
    13981 ttgcacccgt ggaaaggcgt ccaagcatga caccaatgtc actgaaatcg gtgtcagcag
    14041 gattccccat gttatactga taggcggtga cttcgagaaa attcccgcca gtaagccacc
    14101 ggatgatgtc cgcatcgtgt acggcgagat cgacaagtat tccgccgtac aggtcgtcca
    14161 caaacatcca gtccggccgg gttgctcgtc ccagacgatg tggccgccag cacatcacgt
    14221 tggcgacatg gccgacgaac ccgtcggaaa caagctgctt catgcgacgg ttggccgggc
    14281 cgaaccgctc cgtcaacatg agcccgatct taaggcgctt ttcggttgcg gtgcgttcga
    14341 ttgcgttcag ttcatggagt gaagtgcaaa ggggtttgtc ggctatgacg tgttttccgg
    14401 cgttcaaggc gttaagaatc aggggcgcct tcttccggtt gatctctgcc gtccctataa
    14461 gcgtaacatc ggggttatcg agaagctcgg tttccgatgc cgcggcgcgt accggaaatc
    14521 ggctcgttac cgcgcgcagt gaatcctgat cggagtcaaa aacgccggca cactcggtgt
    14581 tcggagcgct gagaagttct ttgatcaaac tgaaaacatg accatggcga caaccgacga
    14641 tgccgaagac aactggcacg gaacatctcc ttcaggcgac aagcgaaatc aagaacgtac
    14701 ggcaaatgta gccctcatcc ccgaaagaac caactggacg caacttggcg tgaccgggaa
    14761 tagaaatccg actttgccgg tataccagta aagaaagagg tttctggtac cgcacgcata
    14821 tcccgcagca ggaaacagga ggttcacgca tgaagccgcg catttctcac agcattccgc
    14881 atttatcggc cgcgttggcg cttgtcttct tcggctgcag ccccgtggag aagacacccc
    14941 cggcgcaaaa cccggctgaa cttccgtcgc cggcgggtgt ggaaaccccc gcggactcgg
    15001 agtacggggg acgggcaacg aaggctcttg cggaggggga gggcatccgt ggaactgcgc
    15061 gcaccagcag caccgcggac atttccgcta cggaaagccg gctgaggggt ctctccgtcg
    15121 atggcgcaca ggcagcccag gtaaaagcag gaacgcacga tgataacgag gaatacccct
    15181 cgtgggtgat gttctcatca cgcaaatggc tccttccgat ggtccttccc gcggcatacc
    15241 acggaagaac cgtgctacgg gtaatcgact ccgcgggcgc tcccgtgatg gcgcaatctt
    15301 acacggtaaa ggacgccggc aatgccccac tgtggcgcgg tgtgacgtat gcgaacggtg
    15361 agacggtcct tttcccgagc gtcttctggt cagaccgcgc gacaccggct tcagtcgtat
    15421 gccacctgtc caacgggcag gaaatccaag caccctggag gccttccctc gatggctttg
    15481 tgacggtgca actggacgcg ccgcgccgga ccccgcgaaa gattccggtg gacgttgcgt
    15541 tcgtgctgga cgcaacgggc agcatgggag acgaaatcca gcagcttcgt gacgtgttgt
    15601 tttcaattca cagccgtctg cagaacgcag cccgagcggc agacatccgc tttggccttg
    15661 tggtgtacag ggatcgaggg gacgccgaac cgttgcgcgt cgtgccgttt accgccaatg
    15721 tggattcgtt tcagctcgct ctggaatcgg tcagcgcgag cggcgggggt gataccccgg
    15781 aggacgtcca cgcaggattg cgggcaacgt tgaaggacct tgcctggcgc gaaggaagca
    15841 tccgcaacgc gttcctcata gcggacgcac ctccgcacac ggattacggg caggaatacg
    15901 attacctctg ggcgagccgc accgccaaca gcatgggcat ccgcatcaac acggtcggcg
    15961 cgagcggcct ggaccagagc ggtgagcacg cgttcaggca gatttccgtg gcaacgtacg
    16021 gtcagttcat ctttctgacc tacggcgaga caggggaaag ctccgggatg ggcagtgctg
    16081 cggaccccgg ccgggtcagc catcacacgg gcgcgaactg gtcagcaaga cgattggacg
    16141 acatcgtcgt tgacctcgtg aggcgcgaca tcgcgtacca gaccgaagta ccgagacttg
    16201 tgaccgacag ccctccgccc gttgaccagc atgaccacct tgcggtgcgg atggagaatc
    16261 tctggcaaca ggttcagagg cagcttgcgg cattcagtgg tgatacgctt acggcggtgc
    16321 tcctgccgtt cgaaagtacc ctcgatgatt cctcccacct cgaggcgtat ctccgcgacg
    16381 tttccacgga aaccctgttg cggctgggct ccgttcgcat ggtggaacgc gaccgcctcg
    16441 cacagatcat cgcggaacag agcctgagcg ttagcgcagc gatcgaaccc tcccgcgcaa
    16501 tcgagctggg ccaccttctg aacagccgcg tcgtgttcct cggcaaaata taccgtcttg
    16561 gaaccgaccg cgtggttcat gttcgtgccg tggaaaccga aacgggtcag attgtggcag
    16621 cggcgcgcgt gcgggtttga acatcgccgg agtatctcac ccgccgtaaa aaaacaggag
    16681 gccggtcagc atcgcgtccg gcctcctgtg tgttgcggtt cgcggcgcct taactgccaa
    16741 attccgcgac gagctttt
//
LOCUS       DAOTNG010000025        16569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3473_length_16569_cov_4.664709, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000025 DAOTNG010000000
VERSION     DAOTNG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3473_length_16569_cov_4.664709"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="P5179_02300"
     CDS             complement(<1..1472)
                     /locus_tag="P5179_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HRS94080.1"
                     /translation="MADSETRLFEAAVRANWSAYQKVIQGLAPAWDYCVITSANERQA
                     RAYESELDWRRLRGKLPPSTRFLVVPDPDGQRIGSGGATLNALRAIFEDKSARGEVAD
                     ESAWSKTRILIIHSGGDSKRVPHLAATGKAFGVLPLLDPEGQPITPFDQLFVAVAGCA
                     GDLSAGMMVLSGDVLLVFDHRAIRANELRGVRGVAFNALEDTAAHFGVFVAAQDGSVR
                     LFLQKPDHQALEVSGAFGSSGEVKADTGILLFDESATAACARLAGLHGGADLVRSARS
                     LGSRIELYEDFAFALARETDHAAYVAGGGACQIRETIWNSLHQFHFDHVTVSPIRFVH
                     IGTTEQYLDVPREAENHGYGYAVARSVYASGTTPPAKSCLIETVLRDNTSVIGEDAVL
                     IGCHLRRATVGRRSILSGLVSPDQDISLGDECVCAVIPLADDESGNYAILLYGLKDNP
                     KLSADEAGCTFCNTAMSSWMQTAGINRADLWDVGDQQTIW"
     gene            complement(1465..2307)
                     /locus_tag="P5179_02305"
     CDS             complement(1465..2307)
                     /locus_tag="P5179_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRS94081.1"
                     /translation="MNAQSTTELAQVPQKALESRFIVGTRVDATSYEDAVDRCISWAR
                     EGESRYVCVSTVHMVMEGHDSPAYQHVVNSADLVTPDGVPLVWALRWLGIKGVTRVYG
                     PELTPQLLKKAADHRIPVGFFGGGTQAALDRLVSIVRERFPHLVITYAYAPPFHPLSP
                     DEDETIVREITDSGARVLFVGLGCPKQERWMHEHRGRLPAVMVGVGAAFDFLAGVKPQ
                     APRLVQKMGMEWLFRLATEPRRLWKRYLKHNPRFVFFFLLQVLGVRQFAPIGAIGLSE
                     GTNG"
     gene            2503..>3557
                     /locus_tag="P5179_02310"
     CDS             2503..>3557
                     /locus_tag="P5179_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HRS94082.1"
                     /translation="MRTWRHRFLLVSMIVIAAWLPAAAVYLPLDNRAYEDIDALALRG
                     YRVVPATTIKPYSRKTVAQGLSHLSDRRADMSAAEREMYDRLLAEFADNIADETASPG
                     PSSRDRWWLKADVNPRAMAQTTNERTDLPRARRVPATRRSLNDRARLIGTLDLTFRLT
                     RNLSTFQRLEMDTDGLWDNDFRGHRVYGEDWGMFNATGQVEEAYITYDNDWGSVGFGR
                     IQRMWGPERQGSIFLSDNSPAMDMLSADVHLNWLHFASITAELEPEQDASGTPIIRYY
                     AGHRLVLTPFRWAEVGLSESAVYGGTNQGFSLRWSNPFVFLYGEEVNINPTSENLLVS
                     ADISLRPVRNTEFYGAFL"
     assembly_gap    3558..3657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3658..4271
                     /locus_tag="P5179_02315"
     CDS             <3658..4271
                     /locus_tag="P5179_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94083.1"
                     /translation="ADISLRPVRNTEFYGAFLIDDVSIDKPKLAPDRIAWSAGARWEA
                     PFGIDRLGAGAEYVRLSRWVYNYARIAPWRHYVNKNAVLGSFLGPDGDALFLEGRWVT
                     QNGTAIKGLLAHRRQGETRYNTPTPNYLPSDYRYPCEPFPFGIVEKRTSLEAIVEFPP
                     VYGASVAGALSGSTTLNRDNKKAPRHWGWGLRLDLDWHIPVRW"
     gene            complement(4278..5543)
                     /locus_tag="P5179_02320"
     CDS             complement(4278..5543)
                     /locus_tag="P5179_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94084.1"
                     /translation="MTRNPAARIWVRTMLAFLLFGFARATCAEQGLPGDSGCTYHSVT
                     FSLIPRVSTTYGHSKPVTNLSINLLVGKHFALRGVEIGGITNVQESYARGLQVAGIVN
                     VAGGNVRGIQSAGIVNVAGQTVRGIQGAGIVNIAGKNLVGTQASLIGCISGNDVRGSQ
                     TAGIFAIAGRSVEGFQGSYVIGISGAGTIGAQTSGIMSISGGGVEGVQFAGIGSIAGG
                     TVEGIQAAGIFAVAPQVRGAQFGMFTAADSVFGLQVGFVNRSKRAEGYPVGLINCVEG
                     VPVKVGFVVDESAGSYVELTTGSLHTHGIVALGGRILAAPQTQAVGLGVGLHFQKTRH
                     YCDIDAITYGNFAADSGGEGAVLALRFILGIPVNRHLDLFAGGRMSLFVSDTGTGAAL
                     APWSLYKGTARGRYVRVWPGLTGGVRVSL"
     gene            complement(5540..6985)
                     /locus_tag="P5179_02325"
     CDS             complement(5540..6985)
                     /locus_tag="P5179_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRS94085.1"
                     /translation="MSGFFSSTAGRRLVLYGLVVLVLALGTAGVLLLLENISQRKYEA
                     RQVFFRVVELNEDTEDPAVWGKNFPNQYDTYLRTVDTVRTKYGGSEAFQKLDAFPVWR
                     ELFRGYAFGIDYREDRGHAYMLSDQRQTDRVTKKPQPGACVQCHASVIPAYKKVGVSQ
                     GGVPDDDAHRADAIMAGFELVNAMPYSEATQLVKHPVTCLDCHNPATMQLRVTRPGFL
                     VGIQRLAESGVSLPHLPSIERWRRGDRSKPYDANTLASHQEMRAFVCGQCHVEYYFKP
                     PNTQLTYPWHNGLRVEHIEAYYDSVGWKDWTHATSGAPVLKAQHPEFETWSQGIHARA
                     GVTCADCHMPYVRVGAVKISDHQVRSPLLGINRACQGCHRAPETELLARAITIQDRTS
                     ALMTRAENATLALVLAIRAAQERGASDEQLTKPRDMHRKAQWRLDFVAAENSMGFHAD
                     QETARILAEAIDYARQGETEVARIGSGRAPR"
     gene            complement(7017..7481)
                     /gene="nrfH"
                     /locus_tag="P5179_02330"
     CDS             complement(7017..7481)
                     /gene="nrfH"
                     /locus_tag="P5179_02330"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HRS94086.1"
                     /translation="MDYTRTARWMSVFAVVLLGIGIGAGAYTLVYARGASYLLDDPQA
                     CVNCHVMREQFDGWEKSSHHAVATCNDCHVAHGPVSKWLTKAENGFHHSVAFTFQNFH
                     EPIQIRESSSLLLQSNCVRCHADITDAITWNHPKTRDRCVRCHDSVGHGPSR"
     gene            complement(7549..8760)
                     /locus_tag="P5179_02335"
     CDS             complement(7549..8760)
                     /locus_tag="P5179_02335"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRS94087.1"
                     /translation="MATQGENPADDQVGAVLESLGLIVQDQRRINILLSRLVHDMDKR
                     VQALEEGGGIAAGRGARASVQIGKDIPAYPPSDFEFLKRTTVFGSLSDDALRVLLHKG
                     EVKTVPKGYVVFETGKPADTLWVIKKGVVEISRVVDGALKTVAYFTDSETIGEMRIIT
                     RSPHRSNGRAPGGGEIFFLTRDQLLECMELVPEIALRMCEMYAHKLEGTVTTLRTHEQ
                     KQRQLEGNLEYFDLATVIQTLLSTDQRTGILFVSDDKQQPVAELFIDQGHVKRASVGA
                     LTGEEAFYQLFLRELKRGNFFFKEDVDPAAETAEIDIPGMSLLMEAARLQDELSAIKS
                     EVITDPDKVYRQTSRTFEWQDDETKALAEVVWRAVGNGLSVRQIVEEIPRNEYTVYSL
                     LQQLIQNGQIG"
     gene            9008..10351
                     /gene="hisS"
                     /locus_tag="P5179_02340"
     CDS             9008..10351
                     /gene="hisS"
                     /locus_tag="P5179_02340"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRS94088.1"
                     /translation="MASALIEPRVLRGFRDYLPEEMIPRQRMINTITAVFERHGFAPL
                     GTPALEYSEVLLGKYGADAEKLLFRFTDNGGRDVSLRYDLTLSLARVAAQYGNLPVPF
                     KRYQIAPVWRAERPGHGRFREFYQCDVDIVGAAGPLAEYECIQVDYDVMTALGIERFS
                     IHVSNRKLLEGLSSKLGVSDGAQSRAIARTIDKLPSQGEGAVRTLLSSDAGLNSEQIG
                     IVFEYLSLKGSNEAILAQLREFFANTPEAHSGIESLEAIIRCASAAGIPEGTIELDLS
                     IARGLDYYTGTVYETFLLDLPALGSVMSGGRYDGLVAIFTGQDVPAVGISVGIDRLFS
                     GLQETGRIPQAATVSQVLVTVFNESTTPQSLQAATRLRSLGVAAEVAVEGGALRRQLK
                     YANKQHIPFALIIGPDEAENGTMTLRDMDSGEQESFSTVEAVAARVLEQPEGHRS"
     gene            10364..11515
                     /locus_tag="P5179_02345"
     CDS             10364..11515
                     /locus_tag="P5179_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94089.1"
                     /translation="MPRGQYCGTERIQRLDRVTGIPVVQVTSMFTPSVHMHYETVTFT
                     PDNSWMIFLSARSTGTGAPMNLVRASSDGKDIVQLSGDSAVSNVVMAEDGSAAFYMQD
                     GAVHRTEIRTAKDEIAVPLRTGEFRPHGATHGMLSMGRYYFAELRSDSCAGIGRWDLE
                     TGQFTEVIRCDNTGHLKANPAGPEISFNVYEYREGTNENGKRNFVKTEEYRHAESLER
                     IEVTFPKGRHGIAHSYWLGGTGRYQGTLQWPERGIAIMERDAPEPEIIPSPGPYFWHS
                     GSSYDGDWIIADTNFPYEGIWLVNVTTGKKELLCRPPLPDGADKVDKPLAHPHPNLSH
                     DGSRAVFTFSDDRSIPQVFVVKIPGDVRRRLGTRNGATREGRMESQVGF"
     gene            11548..12531
                     /locus_tag="P5179_02350"
     CDS             11548..12531
                     /locus_tag="P5179_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRS94090.1"
                     /translation="MSKMDDLQLFDDLQPKLERVRTELANVIVGQKAVIDHLLIGLLS
                     GGHCLIVGVPGLAKTLMVRSFAQALDLAFNRVQFTPDLMPSDITGSEILDETAGSGQR
                     SFRFVRGPVFTNVLLADEINRAPPRTQSALLEAMQEHHVTSSGQTHKLAEPFYVLATQ
                     NPIEQEGTYPLPEAQLDRFMFMLWVDYPSADEEVQVVRSTTGEGSGKVSTVLERDEII
                     EAQALVRRVPVDGNVVLRAVNLVRRTRPVEGSPDFVRDFVSWGAGPRASQYLILAAKS
                     KALLEGRKTPAPEDVDALALPVLRHRIVTNFNAEAAGVKTPDIIARLLESA"
     gene            12528..13493
                     /locus_tag="P5179_02355"
     CDS             12528..13493
                     /locus_tag="P5179_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS94091.1"
                     /translation="MNVESNRAATQYRRFLDPQTLARLKGLDLVARFAVEGFMAGLHR
                     SPFHGFSAEFSDHRQYISGDPIRHLDWKVYAKTQRLYLKRYEEDTNLQAYLLLDRSGT
                     MAYGSDGLSKWDYARMVAASLAHLLLSQRDAVGLALFDHDVRRWLPARSTPGHRDALL
                     AEMDRFPAENPTDPSSALHVLAERVRRRSLIILISDMLIPAGAVAPSSGVTLADAMVE
                     RWVNSLKHFRHQGHDVLVFHVLHRDEVDFEFSGSVEFAGLEGEPGLLVDTWSVAHIYR
                     QQMSTYLRAVEKACGEIHVDYHRLFTSSPLDLALSSCLDKRRRLG"
     gene            13587..15122
                     /locus_tag="P5179_02360"
     CDS             13587..15122
                     /locus_tag="P5179_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS94092.1"
                     /translation="MSKRVLIAVMTGVVCMLARPPGSQAAVLTVLPPAHRFELDSTTG
                     VHYTVLADSGKSTNLYFHQRSWLSDNSVVLYTGPTSAQRLMGYIQATGERFVIGNITG
                     ATAAARRNSVFGMRDRKLVEIALLITISNDPRSVPSRVEATERTIRTLTFTSQVNANY
                     DDTFLSVYENNESTTPKRYSVYRISVSNGEMIEVCRASSSISHLQWSRGPSNLLMYSG
                     NDDARLWVVNPAEGVPRNVYVQVPSMGEWVTHESWWVNDQILWCGAPWPAGYELDPQR
                     GEYHHVNVLDLRSGISRIVAAGSWLPAQENAERWKRNFHHACGDEDGRWIVADSFSGV
                     LTLTEGKTTRTRLFTVGHRTYGGGEHSHPSFDRLGKQVVFVTNRFGEPRVCVARIPDD
                     WRAESAPIITSVEPAAEPPQRRGDLPFSIPALFPSEPNPFNPATLIRFSVPRTERVVL
                     SIYAINGQLVCTLVDATRQAGEHSARWEGRDSSGRLVGAGVYLCRLVTPSGTATERIV
                     VVR"
     gene            15153..16346
                     /locus_tag="P5179_02365"
     CDS             15153..16346
                     /locus_tag="P5179_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94093.1"
                     /translation="MNKTAKLLMFLLVFPGSFLVRAARATDSTYVLTVLPREDRTERD
                     SVTFVDYTYLTTTNPSTGLYFHQRAWLPDNSMVVFESEHGLTGYLMHTGELVVIGRVK
                     GATVAARRNSIFGMRGNNVIEIALAITASANPGENPSQVVATERIIATLPGRSQINGN
                     YNDTFLSAYVAPTIYQISVATGQVREVCRIGPPVKWRGHLQWSRTASNLLSFAGGDDR
                     HVEGGPDRIWVVDPNEGIPRPVYAQIPGELVTHESWWVNDQILFCGAPRAFGFETDSL
                     SGEYSHVKVLDPRTGTVRIIGAGSWMPGVSARDLWTRNWGHCAGSDDGNWVVADNFHG
                     VIALFGGKSSRARLLTVGHRTYGAGAHAHPSFDREGKIVVFTSNRLGEPRVCIARIPW
                     RAGSF"
BASE COUNT         3411 a         4825 c         4804 g         3429 t
ORIGIN      
        1 tcccagatcg tctgctggtc gccgacgtcc cagagatcgg ccctgttgat gccggcagtc
       61 tgcatccaac tggacatggc ggtattgcag aaggtgcagc cagcttcgtc cgcggaaagc
      121 ttcggattat ctttcagacc gtacaggagg atggcgtagt ttcccgactc gtcatccgcc
      181 agagggatta ccgcgcagac gcattcatcc ccgagagaga tgtcttgatc ggggctgacg
      241 agaccgctga ggatagaacg cctcccgacg gtcgcgcggc gaaggtggca cccgatcaac
      301 acggcatctt cgccgattac gctcgtatta tcgcgcagaa cagtttcaat aaggcagctt
      361 ttcgcggggg gtgtggttcc acttgcatac accgatcttg cgaccgcata cccgtatcca
      421 tggttttccg cttcacgcgg gacatcgagg tactgctccg ttgtaccgat gtggacaaat
      481 cggatgggtg ataccgttac gtggtcgaaa tggaactgat gcagagaatt ccagatcgtt
      541 tcccgtatct gacaagcccc gccaccggcg acgtaggcgg catgatcagt ttcgcgagcg
      601 agggcgaacg cgaaatcctc gtacaattcg atcctcgaac cgaggctgcg tgccgacctc
      661 accaaatccg caccgccatg cagaccggca aggcgcgcac atgcggccgt tgcagattcg
      721 tcgaaaagga ggatgcctgt atcagccttg acttcccccg aggaaccgaa tgcgcccgac
      781 acttcgagtg cttgatgatc gggcttctgc agaaacaatc tcaccgatcc gtcctgtgcc
      841 gcgacaaaca ccccgaaatg cgcggccgtg tcttccaagg cattgaaggc cacgccgcga
      901 acaccacgaa gttcgttcgc gcggatagcg cggtggtcga aaaccagcag tacatctccg
      961 gagaggacca tcattcccgc ggaaaggtct cctgcgcaac cggctactgc cacgaacaac
     1021 tggtcaaaag gagtgatcgg ctggccttcc gggtcgagca gcgggagaac accgaacgct
     1081 ttccccgtgg ccgcgagatg aggaacccgc ttactgtccc cgcccgaatg aatgatgaga
     1141 atccgggttt tgctccatgc ggattcgtcc gcgacttcac cgcgggccga cttgtcctcg
     1201 aaaatggccc ggagagcgtt cagtgtcgcg cctccgctcc cgatgcgttg tccgtcaggg
     1261 tcgggaacca cgaggaagcg agtggaaggc ggcaatttgc ctctcaggcg ccgccagtca
     1321 agctcagact cgtacgcacg cgcctgtcgc tcattggccg acgtgatgac acaataatcc
     1381 cacgcagggg caagcccttg gattaccttc tggtacgccg accagttggc gcgtaccgcg
     1441 gcctcgaaca gacgcgtttc agaatcagcc atttgtgccc tcagaaaggc cgatcgctcc
     1501 gattggagcg aattgcctca ccccgagaac ctgcaacagg aaaaaaaaca cgaaacgagg
     1561 gttatgcttg agataccgtt tccagagccg gcgaggttcg gttgccaacc gaaacaacca
     1621 ttccataccc attttctgga ccaagcgagg cgcctgaggt ttcacgccgg cgaggaaatc
     1681 aaacgcagca ccgacgccca ccatcaccgc aggcaaccga ccgcgatgct cgtgcatcca
     1741 ccgttcctgt ttgggacatc ccagtccgac aaacaagacg cgagcaccag aatccgtaat
     1801 ctccctgacg attgtttcat cctcatccgg cgaaaggggg tggaacggcg gggcgtatgc
     1861 gtaggtgatc accaggtgcg ggaaacgctc ccggacaatc gacacgagtc ggtcgagcgc
     1921 tgcctgagtt ccaccgccga agaagccgac cggtatcctg tgatcagccg cctttttgag
     1981 aagttgtggt gtgagttccg gaccgtacac gcgtgtaaca ccctttattc ccaaccacct
     2041 gagtgcccag acaagaggca cgccgtctgg cgtaaccagg tcggcgctgt tgaccacgtg
     2101 ttggtacgcg ggcgagtcgt ggccttccat taccatgtgc acggtgctta cgcagacgta
     2161 ccgcgattcg ccctcccgtg cccacgatat gcaccgatcg acggcatctt cgtagcttgt
     2221 cgcatcaact cgtgttccca caatgaagcg gctttcgagg gccttctgcg gcacttgtgc
     2281 aagctcggtt gtcgactgtg cgttcattgg cgcgcgtgaa gttttgagaa aagaaaacga
     2341 ggaatacgaa cgaattcgta tcgcaaaata tacgacgaga tacgaccatc gacggtgaca
     2401 aaacacacaa aacgcgggga gagatagtta gagttgttct tgtcgctgca gtttcagaaa
     2461 ttcaatcgat ggttcgactt ttgcgattgg agcgcgtgca ccatgcggac atggcgacac
     2521 agatttctgc tggtatccat gatcgtaatc gcggcatggt tgccagcggc cgcagtgtac
     2581 cttcctctcg acaaccgcgc ctacgaagat attgacgcgc ttgccctgcg tgggtaccgc
     2641 gtcgtgccgg cgacaacgat caagccgtat tcgcgaaaaa cggttgcaca aggcctttca
     2701 catctctcgg accgccgggc tgatatgagc gccgcggaac gggagatgta cgatcgcttg
     2761 ctggcggaat ttgcggataa catcgccgat gaaacggcaa gtcctggccc gtcatccagg
     2821 gatcgttggt ggctcaaggc ggatgttaac ccacgggcga tggcgcaaac cactaacgaa
     2881 cgaacagacc tgccacgtgc ccgtcgcgtg ccggctacga ggcgttctct gaatgacagg
     2941 gcgcgcctga ttggaacgct tgacctgacg ttccggctta cgcgcaacct gagcacgttc
     3001 cagcggctgg aaatggatac ggatgggctc tgggacaatg acttccgcgg gcatcgcgtg
     3061 tacggtgaag actgggggat gttcaatgcc acaggtcagg tcgaggaagc gtacatcacg
     3121 tacgacaacg attggggaag cgtagggttc ggccgaattc agcggatgtg gggaccggaa
     3181 cggcagggat caatctttct ctctgacaat tcaccggcga tggacatgct atcggcggac
     3241 gtgcacctca attggctgca ttttgcttcg attacagcgg agttggagcc ggagcaggat
     3301 gcgtcgggga ctccgatcat tcggtactat gcaggtcacc gccttgtgct gaccccgttc
     3361 cggtgggcgg aagttggcct ttcggaatcc gccgtatacg gaggcacgaa ccagggcttc
     3421 tcgcttcgat ggtccaaccc gttcgtgttt ctgtacggtg aagaagtaaa catcaacccg
     3481 acgagcgaga atctccttgt aagtgccgac atttccttgc gcccggtccg gaacacggag
     3541 ttctacgggg catttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg
     3661 ccgacatttc cttgcgcccg gtccggaaca cggagttcta cggggcattt ctcatcgatg
     3721 atgtctcgat tgacaagccg aagttggcgc cagatcgaat cgcatggtct gcaggtgcac
     3781 gctgggaagc gccgttcggc attgacaggc tgggtgccgg tgcagaatac gtgaggctgt
     3841 cgcgttgggt gtacaactac gcgcgcatcg ctccgtggcg ccattatgtg aacaaaaacg
     3901 cagtactggg gagctttctc gggcccgatg gcgatgcgct gttccttgag ggacgctggg
     3961 taacgcaaaa cgggactgcc atcaaagggc tccttgcgca tcggagacag ggtgagacgc
     4021 ggtacaacac gccgacgccc aactacctgc ccagcgacta ccgttacccg tgcgaaccct
     4081 tcccgttcgg catcgtggaa aagcgcacgt cccttgaggc gattgtcgag ttccccccag
     4141 tctacggtgc cagcgttgct ggcgctcttt ctggaagtac gactctgaac agggacaaca
     4201 aaaaagcccc tcgccactgg ggctgggggc tgcgcctcga tcttgactgg cacatacctg
     4261 tgcgatggtg actcccctca aagactcacg cgtacgccgc cggtcaatcc gggccagact
     4321 cgtacatacc tgcctcgcgc cgtccctttg tacaggctcc atggagcaag cgctgccccg
     4381 gttccggtgt ccgaaacgaa caggctcatc ctgcctccgg cgaacaggtc caggtgccgg
     4441 ttcaccggta ttcccagaat gaaccgcagc gccaaaaccg ctccctcgcc accggaatct
     4501 gcggcgaaat tgccgtacgt gatcgcgtca atatcgcagt aatgccgtgt cttctggaag
     4561 tgcaacccca cccccaaccc tactgcctgg gtctgtggcg cggcgagaat tcgtcccccc
     4621 agagcgacga ttccgtgagt gtgcaggctt cctgtggtca attcgacgta acttcctgca
     4681 ctctcgtcga caacgaaacc taccttgacc ggaacacctt ccacgcagtt gatcaggccc
     4741 accgggtacc cttccgcccg cttggatcgg ttcacaaatc ccacctgaag cccaaagacg
     4801 gaatccgccg cggtgaacat gccgaactgc gcaccgcgca cctgaggagc caccgcgaaa
     4861 atgccggctg cctggatacc ttcaacggta ccccccgcga tggaaccgat accggcgaac
     4921 tgcacgccct caacgccgcc acccgaaata ctcataattc cgcttgtttg agcgccgatg
     4981 gtaccggcgc cggaaatgcc gatgacataa ctgccctgaa acccttccac actccgccct
     5041 gcaatagcga agatacctgc cgtctgggac cctcgcacgt cgttgcctga gatgcagccg
     5101 atgagcgaag cctgtgttcc gacgaggttc tttcccgcga tgttgactat tcccgctcct
     5161 tggattcccc ggacggtttg cccggccaca ttcacgattc cggcagattg tatgcctctc
     5221 acgttcccgc ctgctacgtt gactataccg gcaacttgga gcccgcgggc gtaggactcc
     5281 tgaacgttcg tgatgccgcc gatttccacc ccgcgcaacg cgaaatgctt ccccaccagg
     5341 aggttaatgg agaggtttgt taccggtttg ctgtgtccgt acgtggtcga gacacggggg
     5401 atcagcgaga aggttacgga gtgatacgtg cagccgctgt cgcccggcaa accttgttcc
     5461 gcgcacgtcg cccgcgcgaa gccgaacaaa aggaatgcca acatcgtgcg gacccagatc
     5521 cgggccgcgg gattcctcgt catcggggtg cccttccaga accgatgcgc gcgacctcgg
     5581 tttccccctg tctcgcgtag tcaatcgcct cggccagtat tcgggcggtt tcctggtcag
     5641 catggaaccc catgctgttc tccgccgcaa cgaaatccag tctccactgc gccttccggt
     5701 gcatatcgcg cggctttgtc agttgctcgt cgctggcccc tcgttcctgc gccgccctga
     5761 tcgcaaggac gagtgcgagc gtggcattct ctgcgcgcgt catcaaggca ctcgtccgat
     5821 cttgaatcgt aattgcacgt gcaagcaatt cagtttccgg cgcgcgatga cagccctgac
     5881 aggcccgatt gattccaagg agcgggctcc ggacctggtg gtcactgatc ttcactgccc
     5941 ctacgcggac atacggcata tggcaatcag cgcaggttac ccctgcacgc gcatggattc
     6001 cctggctcca cgtttcaaat tccggatgtt gggccttcaa caccggtgca cccgaagtcg
     6061 cgtgtgtcca gtctttccaa cccaccgaat cgtaatacgc ttcgatatgt tcgacccgga
     6121 ggccgttgtg ccagggatat gtgagctgag tattcggggg tttgaagtag tattcaacgt
     6181 ggcactgccc acacacgaag gcgcgcatct cctgatgaga tgccagagtg tttgcgtcat
     6241 acggtttgct gcggtcgcct cttcgccagc gttctatgct gggcagatgt ggcagggaga
     6301 ctccggactc ggcaaggcgc tgaattccga cgagaaaccc cgggcgcgta acgcgaagct
     6361 gcatggtcgc cgggttgtgg caatctaggc acgtaaccgg gtgcttgacg agttgcgttg
     6421 cctcagagta aggcatagcg ttgaccagtt cgaaccccgc catgattgcg tccgcccgat
     6481 gagcatcgtc atcgggtacg ccaccctgcg acaccccgac tttcttgtag gcgggaatga
     6541 cggaagcatg gcactgaaca cacgcgccgg gttgcggttt tttcgtgacg cggtccgtct
     6601 gtcgttggtc ggaaagcatg tacgcatgcc cgcggtcctc gcggtagtca atgccgaaag
     6661 cgtatccccg aaaaagttct cgccacacag gaaatgcgtc cagtttctga aaagcctcgc
     6721 tgccgccgta tttggttcgc accgtatcga cggtccgcaa atacgtgtcg tactggtttg
     6781 gaaagttctt tccccatact gccgggtctt cggtgtcttc attgagctca accacacgga
     6841 aaaaaacctg tcgtgcctcg tacttcctct gggaaatgtt ctccaagagg agcagcaccc
     6901 cggcagttcc gagcgcaagg acaagtacga caagcccgta cagcacaagt cgccttccgg
     6961 cagtggaaga gaagaagccg ctcatgacat ccctcactga ctggggttac agaagcttat
     7021 cgactcggcc catggcctac agaatcgtgg caacggacgc agcgatcgcg agttttcgga
     7081 tggttccacg tgatagcgtc tgtgatgtcg gcgtggcagc gcacgcaatt gctctggagc
     7141 aggagcgaac tggattcgcg aatctggatg ggttcgtgga aattctgaaa agtgaaggct
     7201 acggagtggt gaaacccgtt ttctgccttc gtgagccatt ttgagacggg cccatgcgcg
     7261 acatggcagt cattgcaggt agccacggca tgatgagagg acttctccca cccgtcgaac
     7321 tgctcgcgca ttacgtggca gttgacacag gcttgcgggt cgtcgaggag atacgacgca
     7381 cctctggcat ataccagagt gtacgccccc gccccgatac ctattccgag caatacgact
     7441 gcaaagacag acatccagcg agcagtgcgc gtgtagtcca tgcctgcatc cggagggttg
     7501 cggacacacc gagcgggtac gcaggtcgtc ctggtgaaac gacgaatgtc aaccgatctg
     7561 cccgttctgg atcaactgct gcaacaggct gtacaccgtg tactcgttgc ggggaatttc
     7621 ctctacgatc tgcctcacgc tgagaccgtt tccgaccgct ctccagacga cttccgccaa
     7681 tgcttttgtt tcgtcatcct gccactcaaa cgtgcgggaa gtctgtcggt acactttgtc
     7741 cggatcggta atgacctccg atttgatcgc actcaactcg tcctgcaatc gtgctgcttc
     7801 catcagaagg ctcatgccgg ggatatcgat ttccgccgtt tcggcagccg ggtcaacgtc
     7861 ctccttgaag aagaagttgc cgcgtttcag ctccctcagg aaaagctggt agaatgcttc
     7921 ctcccccgtc aaagctccca ccgaggctcg tttgacgtgt ccctggtcga tgaacaactc
     7981 ggcaactggt tgctgcttgt catcgctcac gaagagtatg ccggtgcgct ggtcggtgct
     8041 gagcagagtc tgaatgacgg ttgcgaggtc gaagtactcc agattgccct caagttgccg
     8101 ctgtttctgc tcgtgcgtgc ggagcgtggt tactgtgcct tccagcttgt gggcatacat
     8161 ctcgcacatg cgaagtgcga tctccggaac aagctccatg cattcgagga gttgatcgcg
     8221 tgtcaggaaa aagatctccc cgccgccggg agcccggccg ttcgaacggt gcggggatct
     8281 tgtaataatg cgcatctcgc cgattgtctc agaatcggtg aaataggcga cagtcttcag
     8341 ggccccgtca acaaccctgc ttatttcgac cacgcctttt tttatcaccc agagggtgtc
     8401 tgcgggtttg cctgtttcga aaacaacgta ccccttcggc accgtcttca cctcaccctt
     8461 gtgaagcagc acccgtaacg catcgtcgct caatgacccg aatacagtag ttcgcttgag
     8521 aaactcgaaa tcgctcggtg gatacgccgg aatgtctttt cctatctgca cactggcacg
     8581 cgcgccccgc cctgcggcga tccctccgcc ttcttccagc gcctgcacgc gtttgtccat
     8641 atcgtgcacc aggcgcgaca ggaggatgtt gatccgccgc tggtcctgaa cgatgaggcc
     8701 cagggattcg agcacagcac caacctgatc gtccgctggg ttttcccctt gagtcgccat
     8761 ggtgtacttc ctccgtgacg tttccgtgta cgtcgggttc tgaactgacc ggtgcgagaa
     8821 cctccctttt ctgcaatcca gagccaacaa tacaaaaaca gccaccggga tcaaagtgcg
     8881 gcggtccaga ctcgggaaag gtctgacgcg ggtctgtggc ggattgcccc gatgcgcgcg
     8941 gggaacgatt tttaggttcg cacgtattcg cctttacaag ttgcagtcaa ccggagcaag
     9001 catacctatg gcgtctgcat tgatcgagcc tcgcgtgttg agaggattcc gcgactatct
     9061 ccctgaagaa atgatccccc gtcaacgcat gatcaacacc atcacggcgg tgttcgaacg
     9121 gcacggtttt gcgccactcg gcactcccgc gcttgagtac tctgaggtac tcctcggtaa
     9181 atacggtgca gatgccgaga agctcctctt ccgcttcacc gacaacggcg ggcgagacgt
     9241 aagcctgagg tatgacctga cgctctcgtt ggctcgcgtg gcggcgcagt acggtaacct
     9301 gcccgtgcct ttcaagcggt atcagatagc cccggtctgg cgcgcggaac gccccggtca
     9361 tggtcgcttc agggagttct atcaatgcga cgtggacatc gtcggtgcag ccggtccgct
     9421 cgcggaatac gagtgcattc aggttgatta cgacgtaatg acggctctcg gcatcgagag
     9481 attctccatc cacgtctcca acaggaaatt gctggaaggg ctttcgtcga aactcggggt
     9541 ttccgatggc gcacaaagtc gcgccattgc ccgtaccatt gacaagttgc cgtcgcaagg
     9601 agaaggggcg gttcgtaccc tcctttcttc ggatgccgga ctgaattcgg agcagatcgg
     9661 gatcgtattc gagtatctct cgctgaaagg aagcaacgag gctattttgg cgcaactgcg
     9721 agagttcttc gcgaataccc cggaagcgca ttccggcatt gagtccctcg aggcgataat
     9781 ccgctgcgcc agcgcagccg gaataccgga gggcaccatc gaactggacc tgagcatagc
     9841 ccgtgggctt gattactaca cgggcaccgt gtacgagacg ttcttgctcg atctccccgc
     9901 cttggggagc gtcatgagcg gcgggcgtta tgatggcctc gtcgcgatct tcacgggcca
     9961 ggatgtcccg gccgtcggaa tttcagtcgg aattgaccgc ctcttctcag ggctccagga
    10021 gactggcaga attcctcagg cggcaacggt ttcgcaggtg ctggttacgg tattcaacga
    10081 gtccacaaca ccgcaatctc tgcaggctgc gacccggctc cgttccctcg gtgtggcggc
    10141 agaggtggcg gtcgaagggg gagcactgcg caggcaactc aaatacgcga acaagcagca
    10201 catacccttt gcgctgataa tcggccctga cgaagcggaa aacgggacaa tgacgctccg
    10261 ggacatggat agcggcgagc aggagtcctt ttcgaccgtc gaagcagttg cagctcgtgt
    10321 tctcgaacaa cccgaaggtc atcgctcctg acggagttcc tccatgccac gcggccaata
    10381 ctgcggtacc gagcgaatcc aaagactgga tcgtgtaacc gggattccgg tggttcaggt
    10441 caccagcatg ttcaccccaa gtgtgcacat gcactatgaa acggttacgt tcacaccgga
    10501 caacagctgg atgatcttcc tttctgcccg ttccaccgga accggcgcgc cgatgaacct
    10561 tgtccgtgcg agttcggacg gaaaagatat agtgcagcta tccggggatt ccgcggtatc
    10621 gaatgttgtc atggcggagg acgggtcggc ggcgttttac atgcaggacg gcgccgtcca
    10681 caggacggaa atccgaaccg cgaaagacga aatcgcagtg ccgctgcgga ccggagagtt
    10741 ccgtccccac ggcgcaacgc atggaatgct ctcgatgggg cgttattact tcgcggagct
    10801 caggagcgat tcatgcgccg gcatcgggag atgggacctg gaaacggggc aattcacgga
    10861 ggtgatccgg tgcgacaaca ctggccacct gaaagcgaac ccggccggac cggaaatcag
    10921 tttcaatgtg tatgagtacc gagaggggac aaacgaaaac ggcaaacgca acttcgtgaa
    10981 aaccgaggaa tatcgccacg ctgaatcact ggagagaatc gaagtaacgt ttcccaaagg
    11041 gcggcacgga attgcgcatt cgtactggct cggaggaacg ggcagatatc agggaacact
    11101 gcaatggccg gagcgcggca tcgcgataat ggaacgggat gcgcctgaac ctgagatcat
    11161 tccttccccg ggcccctatt tctggcattc gggctcatcg tatgacgggg actggatcat
    11221 cgcggatacg aacttcccgt acgaagggat ctggcttgtc aacgtgacca cgggcaaaaa
    11281 agagctgctt tgccggcctc ctctgccaga tggcgcggac aaggtggaca agccactcgc
    11341 ccaccctcac cccaatcttt cgcacgatgg ttcgcgtgcc gtgttcacgt tttccgatga
    11401 ccggtccata ccgcaggtat ttgttgtgaa aatcccggga gacgtgcgca gacgcctcgg
    11461 cacccggaat ggtgcgacga gagaaggtcg aatggaatcc caggtcggtt tctgagtgcg
    11521 gaacgacagt gtcggcaaaa ggaagtgatg tccaagatgg atgatctcca gttgtttgac
    11581 gatcttcagc cgaagctcga acgggtgaga acggaactcg cgaacgtcat cgtcgggcag
    11641 aaagctgtga ttgaccacct cctcatcggt ctgctttctg gcgggcattg cctcattgtt
    11701 ggcgtgccgg gtctcgcaaa gactctgatg gttcgttcat ttgcacaggc tcttgacctt
    11761 gcgttcaatc gcgtgcaatt cactccggat ttgatgcctt cagacatcac aggaagcgaa
    11821 attctggatg agacggccgg atcaggccag aggagtttcc gattcgtgcg cggtccggtg
    11881 ttcacaaacg tgctcctcgc agacgagatc aatcgcgctc cgccgcgaac gcagtcagcg
    11941 cttctcgaag ccatgcagga acatcatgtg acaagtagcg ggcagacgca caagctcgca
    12001 gagccattct acgtcctggc gacacagaat cccattgagc aggagggaac ctatcctctt
    12061 ccagaggcgc aattggaccg tttcatgttc atgttgtggg ttgattaccc gtccgccgac
    12121 gaggaagtcc aggttgtccg ctccacaacc ggggagggtt ctggaaaggt atccacggta
    12181 ctcgagcgtg acgaaatcat cgaagctcag gctctcgttc gcagagtccc cgtggatggg
    12241 aacgtggtcc tgcgggcggt gaacctcgtg cgacgcaccc gtccggtgga aggcagtccc
    12301 gatttcgtga gggatttcgt gtcgtggggg gccggaccgc gggcaagtca gtacctcatt
    12361 ctggccgcca agtcgaaagc cttgttggaa gggcgcaaga cccccgctcc ggaggatgtc
    12421 gacgcgctgg cgcttcccgt tctgcgacac cgcatcgtca cgaacttcaa cgcggaggcc
    12481 gcgggcgtga aaacaccgga catcattgcg cgtctgttgg aatccgcatg aacgtcgaga
    12541 gcaatcgcgc tgcaactcag tatcggagat tccttgatcc gcagacgttg gcgcgtctga
    12601 agggtctcga tctggtcgcc cggttcgccg ttgaagggtt catggcgggg ctccacagaa
    12661 gtccttttca cggcttttcg gccgagttct ccgaccatcg ccagtacatt tccggtgatc
    12721 ccatccgaca ccttgactgg aaggtatacg caaaaaccca gcggctctac ctgaaacgat
    12781 acgaagagga tacgaatctc caggcctacc tgcttctcga ccgttcaggt actatggcgt
    12841 atggaagcga cggcctcagc aaatgggact atgcccgcat ggtggcggcc tcgttggctc
    12901 atctgctgct ttcccagaga gacgctgtcg gcttggcgct gttcgaccac gacgtgcgac
    12961 gttggctgcc cgcccgttcc acgcccggtc atcgtgatgc cctccttgca gaaatggacc
    13021 gcttccccgc agagaatcct accgatccct cgtcggcgct tcatgttctg gcggaacggg
    13081 tgcgcaggag aagccttatc atacttatca gtgacatgct gattcccgcc ggtgcggttg
    13141 ccccttcctc aggcgtcacg cttgcggacg ccatggttga gcggtgggtg aactcgttga
    13201 aacatttccg gcaccaggga catgatgtgc tggtgttcca tgtactgcat cgcgacgagg
    13261 tggatttcga attctcaggt tcggtggagt ttgcgggtct cgaaggagaa cccggtttgt
    13321 tggtggacac gtggtcggtt gcccacatct accggcaaca gatgagcacg tatctgcgtg
    13381 cggtagaaaa agcgtgcggg gagattcacg tggactacca ccgtcttttc acttcctctc
    13441 cacttgatct cgcgctgtct tcatgcctgg acaaacggcg gcggttgggg tagcgacgtg
    13501 gccggggtgc aggttcactg cgttcgggcc ggtttgttcc agagtggcgt tcagattccg
    13561 gttcttcgca gcaggggcga cgggacgtga gcaaacgagt tctcatcgcg gtgatgacgg
    13621 gcgtcgtctg catgctggcg cgtccccccg gatcgcaggc ggcagtcctc accgttctgc
    13681 ctcctgccca tcgcttcgag ttggattcca ccacgggtgt gcactacaca gtgctggctg
    13741 actcgggcaa atccacgaat ctgtacttcc accagcgctc gtggctttcg gataactcgg
    13801 ttgtcctcta caccgggccg accagtgccc agcgattgat ggggtacatt caggctaccg
    13861 gcgaacgatt cgtcatcggc aatatcaccg gcgcaaccgc ggcggcgaga cgcaactcgg
    13921 tgttcggcat gcgcgacagg aagcttgtcg aaatcgcgct gttgatcacc atatcgaacg
    13981 atccacgaag cgtgccttca cgggtcgagg cgacggaacg aacgatacga acgctcacgt
    14041 tcacgtcgca ggtcaacgcc aactacgatg acacgtttct ttccgtctac gaaaacaacg
    14101 agagcacaac gcccaaacgc tactcggtgt acaggatttc cgtttcaaac ggtgaaatga
    14161 tcgaggtctg cagagcgagc tcgagcattt cccacctcca gtggagccgt ggtccttcta
    14221 acctcctcat gtactcaggg aatgacgatg caaggctctg ggtggtgaat cctgcggaag
    14281 gcgttcctcg caacgtctac gtccaggtgc cctcaatggg cgaatgggtt acgcatgagt
    14341 catggtgggt caatgaccag atcctgtggt gcggcgctcc gtggcccgcc gggtacgaac
    14401 tcgatccgca aagaggcgag taccaccacg tgaacgtatt ggaccttcgc tcggggattt
    14461 cgcggatcgt cgcggcgggc tcgtggctgc cggctcagga aaacgcagaa cggtggaaac
    14521 gcaacttcca ccacgcctgc ggcgacgaag acggacgatg gattgtcgcg gacagtttca
    14581 gcggggtgct gacgcttacc gagggaaaaa ccactcgaac gcgacttttc accgtcgggc
    14641 accgcacgta tggcggcggc gaacactccc acccgagttt cgaccggctc ggaaagcagg
    14701 tcgtgttcgt cacgaatcga tttggtgaac cgcgggtatg cgtggctcgc atccctgacg
    14761 attggagggc ggaatccgcc ccgattatca cgtccgtgga acccgcggct gagccaccgc
    14821 aacgacgagg cgatctcccc ttttccattc cggccttgtt tccgagtgaa ccaaacccgt
    14881 tcaacccggc cacgctgata cggttctcgg ttccgcggac ggagagagta gttctgagca
    14941 tctatgcgat taacggccag cttgtctgca cgcttgtgga cgccaccagg caagcgggcg
    15001 aacattccgc ccgttgggag ggccgcgatt ccagtggtcg ccttgtgggc gctggagtat
    15061 acctgtgtcg gctcgtaaca ccgagtggga ccgccacgga acggattgta gtcgtgcggt
    15121 gaacggcttt cgtgcgtaag gaaaaggaca gaatgaacaa aaccgcgaag cttctcatgt
    15181 ttttgctcgt gtttcccggg tcctttctgg tgcgcgcagc gcgtgcaacg gacagcacgt
    15241 acgtgctcac tgtgctccca cgagaggatc gtacagaacg cgacagcgtt acgttcgtgg
    15301 actacacgta tctgaccacc accaatccgt cgaccggtct ctattttcac cagcgtgcat
    15361 ggctgcccga caattccatg gtggttttcg aaagcgaaca cgggttgacc gggtatctga
    15421 tgcacaccgg agaactggtg gtgatcggac gtgtgaaagg cgcaaccgta gctgcgaggc
    15481 ggaactccat ttttggcatg cgtggtaaca acgtcatcga aattgcgctt gctatcaccg
    15541 cgagtgcgaa tcccggtgag aatccgtctc aggtggtggc aacggaacga attattgcga
    15601 cgctgcccgg cagatcgcag atcaatggca actacaacga caccttcctt tccgcgtacg
    15661 tcgcgccgac aatttaccag ataagtgtag cgacagggca ggtacgcgag gtgtgtcgca
    15721 tcgggccacc ggtcaaatgg cgcggtcacc tccagtggag ccgtacggcg tcgaatcttc
    15781 tgagcttcgc cggcggagac gacagacacg tcgagggtgg acctgataga atctgggttg
    15841 tggatccgaa cgagggtatt cctcgacccg tgtacgccca gattcccggc gaactcgtaa
    15901 ctcacgagtc ctggtgggtg aatgaccaga tccttttctg tggtgcgccg cgcgcgttcg
    15961 gattcgagac cgattcgttg agcggcgaat actcccatgt gaaagtcctt gacccgagga
    16021 ctggcacagt caggatcatc ggtgcgggat catggatgcc cggcgtatcg gcacgggatc
    16081 tttggacgcg gaactggggg cactgtgcag gtagcgacga cggaaactgg gtcgttgcgg
    16141 acaatttcca cggggttatc gcgctgttcg gcggcaaatc ctcgcgcgcg agattgttga
    16201 cggtaggcca ccggacatac ggggctggtg ctcacgcgca ccccagcttt gacagggaag
    16261 gcaaaattgt cgtgttcact tcgaaccgac tcggtgagcc tcgtgtctgc atcgcacgta
    16321 tcccgtggcg cgcaggatcg ttctgaccgc gttaccgggc ggcaaccctc cgaacaggct
    16381 gcaaccacag acccgggggt acgcctgctg tgagaacata tggcgccaaa cgggggacgg
    16441 ggtattttgt cggttacgct ttcttaccgt ttgaaacgaa accccgctga cagaggaaat
    16501 ccagaatctg ctccgtattc atttccggcg tgcaggcgac ggtgtccaac attacctccg
    16561 gattgaccg
//
LOCUS       DAOTNG010000026        16352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3537_length_16352_cov_4.771983, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000026 DAOTNG010000000
VERSION     DAOTNG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_16352_cov_4.771983"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="P5179_02370"
     CDS             complement(<1..1028)
                     /locus_tag="P5179_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRS94094.1"
                     /translation="MSFEALSACVLGLIALAVGARFLVDGSSRLASALGISPLLIGLT
                     VVAFGTSAPELAVSVHAAWAGQPDIAVGNAVGSNIINVLLILGASAVITPLVVSQQLV
                     RMDTPLMIGACVLVYVFAADGLLSRVNGLILFACVVAYTVFAIRVSKRESAGVEQEYA
                     REYCKAKVTGARTWVVMLAAIVAGLGLLVLGSRFLVEGASEIARAYGFSELVIGLTIV
                     AGGTSLPEFATSIAASARREPDIAVGNVVGSNLFNMLAVLGLSSVFSPAGIPVSRAAI
                     AFDIPVMIGVCVACLPIFFTGYRIARWEGALLLGYWFAYTAYTVFEARGHEASSHLTV
                     VMMEFVIP"
     gene            1539..>2524
                     /locus_tag="P5179_02375"
     CDS             1539..>2524
                     /locus_tag="P5179_02375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS94095.1"
                     /translation="MKTLPGDEIRQVQWRFSDRFDLQMLVQSARSVARGLVARLVASG
                     ARNTHEWTEEKAQMLTAFDEAGITSVFMDPEDGGYIEGPKNFALALLAFELAWVDGGA
                     ATGSLAGNLALEPIKEKGTEQQRSHYMKLCVPPAPGDTRKAWRGAFALTEPIPYVGVD
                     TGVLGGKVGIARWNDGEEPILHVEKRGRFITNMGFANFVCAAVDSDDPRIKGSCMVIL
                     EEGDPGVFDRGTPTKKLVHQLSSTTDPVFSLDIPASRIIGGYSVKDGVIVPQWNHSQI
                     IESVFRRTRVTVGLMTSAKLLSAIEPVIRYQRGRFRGGASGAEGTPRHDLGL"
     assembly_gap    2525..2624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2625..3605
                     /locus_tag="P5179_02380"
     CDS             <2625..3605
                     /locus_tag="P5179_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS94096.1"
                     /translation="GRDSTGVASLPDDTLVRFAITDAITNVLCPATKLWNTGHGANMM
                     REAVSLMGGYGITEDCPGFLGMKWMDAQLEATYEGPEAVQRRQLSVTMANELFLTIFR
                     GWIREMRGIASEYPETGACTLASAMELWLWSLTYLLEGKDADGRPLFKDQRQGVTFPM
                     ADALSWLLASRCQILDTIELAARGAERGLDAEALPGYVRFFSDLCHVQAARAAGETSR
                     VCSLLVYGYNRHPGWKDEGACVTDEELVSAESLIPGSAAGVAGTADSIGADGTRATKA
                     GPCAGHEGLETFQRLQKRLHACLSGSALAKDRAARALTSVSIPEALDYPA"
     gene            3653..5533
                     /locus_tag="P5179_02385"
     CDS             3653..5533
                     /locus_tag="P5179_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HRS94097.1"
                     /translation="MGPDLEKGTENAVDRLNLEVDIACVGFGPAMGGFLTTLAREMEQ
                     ASGEPHVQSTVLPGMPLQVVCYERADDVNFGVSGLVTEARAIRNTFPEMNSSEIPLAH
                     PVSEEKIVYLLDPHGASRRSLGLRIADSAIRGLRWVLPYRRSALELPFTPPFLKKHGG
                     LVMSIGQFMQWVSNQVSSSGMVQIWPGTPVGEPILEGKQVVGVRIADQGVQKDGTPDV
                     GYMPGMDVRARLTVVGDGPVGAVGQRLDEQFGLPEGHHQHDWAVGMKVVVSLPDHCDL
                     VPGTVLHTLGYPEPEIFGFLYVHPERIASLGIFVPSTLASPVRTVYRYLQHWMLHPYL
                     WHHLEGGTLRSWGAKTLQESGRLGEPYLAGDGYARIGEGSGSTNVLTGSGVDEAWMTG
                     VLLAEGVIDMLKKGEPFTQANLDAAYVNRRRKSWVETGSRKAQHARDGFRKGFVAGVL
                     GMAVSGLTRGALNLKTRSAHPREGIPDLNQFYRDRIPSEELQRIREECAKNAIPLHDA
                     LMTRAGWPEIPLDGKLLVSQQDALLLGGKVQAAPGFADHVMFVDPAVCEQCGTRLCIE
                     ACSAQAIAPSPSGGTPEFDREKCIHCGACIWNCTQPRPGDRERGNIRFYAGAGGLHSA
                     EN"
     gene            5558..6367
                     /locus_tag="P5179_02390"
     CDS             5558..6367
                     /locus_tag="P5179_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRS94098.1"
                     /translation="MESGYHIVVCGSLVPDPLQTLEPDVGPKGPALKNEMVLPSVLDP
                     WAAHALYEAAHLAKEYAGSKVWLVSVGPKQRLQQLMMTVAQKAPFSFVPVDGSASGFC
                     DPFETATALANAIGAIPDLDRSRLLLFGGYASASRDAGVTLQVVGEMLGITEVFLAVD
                     SLSVSENGEISLLERVEGGNYLFSECDCPPLVVGWATGHLPEPPNNPQIGMANMRSIM
                     PALQKAPQVQVGAEGVSFAKVELPQQQRTTRIVKDVPVEQIAGEIVAWIRG"
     gene            6394..7437
                     /locus_tag="P5179_02395"
     CDS             6394..7437
                     /locus_tag="P5179_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HRS94099.1"
                     /translation="MKKILLLAFVEENGALGKPALEALSGACELAQAFGAALNVGLIG
                     EFAQKAADSIASCGANQILAVQGPEFASARCATDSAAAVALIRAAEADFVLAPQTTRS
                     ARCMPGAAFRAKGRIDTRITGLRVDEGTLLIQRWYYRQRMVAELTRSQRPWVVLLEAG
                     VFNPWSEGSGHAAVSIVPVEPVETRTRIAGIEPASAGTQTIRPDADLLFVAGAGWTKK
                     QTDGEVHVEEAENLIRSFLDLTHASLGSSKSLVDQSGEGQKVISLLTHMHQVGQTGST
                     PRHPKGLATCCHGEEPHVVGWRFINERRAVNLDPNCGWARGKADVLYVADAFAVMGKV
                     IELLGKDTGQAAV"
     gene            complement(7474..14832)
                     /locus_tag="P5179_02400"
     CDS             complement(7474..14832)
                     /locus_tag="P5179_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS94100.1"
                     /translation="MFSRLRQRVYLLVAAMCVAATSVYAVTPPGTLITSQASLTYFDN
                     GGFQFSLLSNTVVTPVLGSVVGTDGTISQTTQIVAGENITVLLTDPDRNYLPNAIDTV
                     HVTVTNPRTGEVETLVLYETGQGTGVFSGSLPTYYGQSAGPDHDGTLFAQPGDVLQIS
                     YNDNATSAGGTAVVTGQTTVLPTWIKLTPEPRTIVANGTDQSRLTARVTDDLDRPLPD
                     GTIVVFTADKGKFSNDTQRIEVPVSGGNGEAVTILTAPILAANDTARVVASFRGFDSD
                     VIKLIILPGAVAVRVYDQTRNVEVRANDPALRVEVELVGTTVTGDPISIFVELDENGV
                     FVVPDIPPGTYQLHVHVTDRVTGADVMVGVLQTIVVNMDGSTSPPRNAVAGMVHARNE
                     ESGARYAGLTVELLDANGNVVATTVLDAMGRYDFQNLLPGAYSLRVKMEDGDVFTVPV
                     SSRTNNLGEVVLNADVLIDPFGRVFDAVTTALIPGSTVTLRNLDERVLAIPLLNGTGV
                     PPNVNNINPFVTPADGRYAFLFSSSQVGTVANPARYIMTVDPPSGTTYPPRRMHLTVR
                     PTADGSATIMMTVSSGDGLQIARPNSFGLTNGPVTVPDIETVAYNIPMFPKTPVLKFS
                     KLASTDTTSYGETVDFAIVVVNVGNDVAPSVTVTDTLNSGWTLVSSDATTSNGGVLTW
                     NVGDLQPGASDTLHVRAEAADQGVILTNAAWAQFTGGLPVSSQADVVSRSASSISITK
                     AVVTPPALVGKEIHYEIRLKTPPGYAGKIAVEDPLPAELVYIAGSSVPAATYDAGTHT
                     LTWQVSGATSDTVRTFTFATEPRGDLTPGDYPIPNTATASVGAFKISSNRADDLVTVP
                     YFRITKTSDITAAEPGDFVVYRIALENLSTADSLSNIVVRDVMPFGFEYVPNTARLDG
                     VAAPPDTLRDRNIIWNVAGIGAGKTRLLTYRLVLGIGAGAGDGQNTATATASTSRGAT
                     LSAGPATARVFVQPALFTQDQVVLGRAWVDLNGNKVHDDGEPPVPGVVLLMEDGTRVI
                     ADRMGRFSIPEIRTGDHAIRLLEHNIPDSLEPVILGVRSTNDPWMRFVSVSASGMAKA
                     NFPFRVKPRPPAPPPPPPPPPPPALAPPESLTVAVTVERVIVPDSITIPASETIILES
                     GVTFATGKADLRPEGIAVLNDLLPRIKRAQGRQITVSGHTDPRPIRTRQFPNNMVLSL
                     ARARTVRNWMVQNGVDSTHIAIFGYGPDRPIAPNTTPEGMQKNRRVEINLDYGTEART
                     IPAGTARVRLAIRTSGNAERDTFTISQPLPEDITRPAPDSLRDRIVQHGDSVWAKIAR
                     ERGVFTDLPAPEVSHRTWGSIPLRRTSEQSDGTIRETWDGSIRVPAAVDSSTSLIPQD
                     GAVRTVQAPSVAPAGGGSVSPSVSVPAAAGQPPALPGAGAQPAAASTVADSISPAQAP
                     AVELRRLPTPKERVQRPGQLVWIEYPTADSTVSYRDRLTLTARGQTGMPMTLRLNGRE
                     VSTLSVRGDSRVDFLNIAAPSGPVTLTVTQTFPGGRVFADSIFIHVVGPAARVMLDVS
                     PSTLPADSLSQAEAIAQVLDEWGMPLADGQVVTFEIDNGSIITPDLYPEERGVQVQMR
                     GGYASALILSSAKPGEATLTANASNIVARATLNYTVPHERFVLVGIASGQIGWKTNAA
                     PPAGVNFGKDFNSGAYGKGKAAFFGRGTVRNGYLLTTSFDSDRRFDDQVYRYLTPERA
                     YPIHGDASSIFYEAPSASKFFFRLAKERNYLQYGDFAPDLSQAELTAYRRTFTGVAAA
                     MRNRHTAWSLFGAFTEQAIQVDEIPGEGVSGNYYLSAARRGVRVVEGSERIVIQTRDR
                     IHPENILREEVQYRFVDYEIDYLAGTILFKKPIPSRGFDENPIFIVATYEASRALQRH
                     TVGGGRFALHSGDRWEVGTSLVGEGRSTGDYWLSGIDAGYRPFQNVFVNAELARSDLA
                     QTGWAWKFGAQGRYRSTLNYDLSYRSAERAFYNPSSTSALPGVTKMRGKVTWSPVAFA
                     SLTGEAFRSDDVVNREVRNSAALGGTYRWRTLTGLASTEITNVDRAGQETRTGVVSTG
                     LEWAASRTLTFKAERDQNFLDEDVTYRPTLNRLQARWSPLARLDVIAEHAFRDYNFVD
                     SSYTAIGLQSRVTDNTTAYANYKLDGGINGQKNEAIVGLRHRFMPFPYVSMSTGFERM
                     QTLRGNRSADFYSYSIAGEYLPPELFKASARFEQREGQTLDKMVVSGAFDVVLARNLS
                     LLAKHTYAGEATFATGTWNRLRSHHFLSGLAWRSDRSDYLNVLGKYEYKKQYNGMLTP
                     ATDRSVHIGSLEGMLEPRSQLEVFGRYAFKVAQLSSEGVSSRSLTDLWMGSIRYELRE
                     TWDVLGEYRLISQHTANDLQHGASAEVGRVLGGNARIALGYNFVGYHDSDFAGLSYWG
                     QGPFMRIQVKFTEREVAGALDGLQTFWRGR"
     gene            complement(14935..>16352)
                     /locus_tag="P5179_02405"
     CDS             complement(14935..>16352)
                     /locus_tag="P5179_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HRS94101.1"
                     /translation="ALSSGWTAVIYGDPNGNGILDAGEMVPGNIVSTIASLPADSSKK
                     VFVVVNVPTGALDGSSQAVTLTATSQFNTGVSDAGVFTSTVSTAVLSMVKTASPSDPK
                     PGDVVTYSIRGNNTGTSTAYGIVFSDVVPSGMTYVPGSIRVFAGTGGTYATATPQTDA
                     TGDDSADFGITAANTVTINWGDSPAGQEGWLFFQATVNAGVAAGTSISNVASVLYSST
                     SGGPLLPAVSSTPGDITVAAKPYVTLSNTSLALTGQPGDTLLYRFSVTNGGNSNDVFD
                     FTTSSTQGFTNQIWLDANNNGIAGDDGDVLLTDTDGDGKVDTGSIPAGTVAHIIVAVV
                     VTPGTVDMTVDATTVTIASSVDPTVTGTVTLTTTVHGPVLSIVKSVSPTGAQPPGTVL
                     TYTVVVTNNGHGTATALQIVDMIPTNTTYQAGTIKVNGIARTDAIDGDGARFEGGAIQ
                     VRFTAMGPGSSNTFTFDVKID"
BASE COUNT         3179 a         4898 c         5014 g         3161 t
ORIGIN      
        1 ataggtatga caaattccat catcacgacg gtcaagtggc tcgatgcttc gtgcccacgg
       61 gcttcaaaca cggtgtaggc cgtgtaagcg aaccagtatc ccaggagtag agctccttcc
      121 catcgcgcga tccgataacc tgtgaaaaag atcggaagac aggccacgca cacgccaatc
      181 ataaccggta tgtcgaaggc aattgcagcg cgcgagacgg gaattcctgc cggtgaaaag
      241 acgcttgaca gcccgagcac cgccagcatg ttgaacagat tacttccgac tacgttgccc
      301 actgcaatat ccggttctct ccgcgcactc gcggcaatgg aagtagcgaa ctcagggagc
      361 gacgtgccgc ccgcgacaat tgtcagtccg atgacaagct cgctgaaccc gtacgcacgt
      421 gcgatctctg aggcaccttc cacaaggaat ctggaaccga gcacaagcaa accaagtccc
      481 gcaacaatgg ctgccaacat cacgacccag gtccgggcac ccgtaacctt tgctttgcag
      541 tattcgcgcg cgtattcctg ttccaccccg gcagattctc ttttgctcac tcgtatcgcg
      601 aaaacggtgt aggcgacaac acacgcgaaa aggatgagtc cattcactcg gctcaaaaga
      661 ccatctgccg cgaacacata aacgagaacg cacgccccga tcatgagcgg cgtgtccatc
      721 cggacgagtt gctgcgacac caccagggga gtaataacgg ccgaggcgcc gagaataagc
      781 agtacgttga tgatgttgct tcccactgcg ttaccgacgg cgatgtctgg ttgtcccgcc
      841 catgcggcgt gaacgctcac cgcgagctcg ggcgcactgg tgccgaacgc caccacggta
      901 agcccgatca acagaggaga aatgcccagt gccgaagcga gtctggagga accatcaaca
      961 aggaatcgag cgccgacggc cagcgctatc agcccgagaa cgcaagccga caatgcctca
     1021 aatgacatgc ggtatccgaa atgtgaacag gaagccccgt tcgtgaattg tcagaccaag
     1081 cctgttgtgt tcgtgttttc gcacgcgatg ggtcgcaggg ttctccgttg tgtggtacgt
     1141 cgccgtaaga acaaagattc ccgtgggaag caggcgcgta atgcaaatcg taccataaac
     1201 cgtgacaaca cggtcctgct gcaatcagat tggacctgag tcacgcatcg cgaaacgtgc
     1261 tgcgcgtcgt atctgctcgc ccttgactgc tccttcgcgg attatgacct taataaacgt
     1321 cctgggcgtg attgcagtcg gcgcaattca aaacatacgc acctgattca cccgcgcgca
     1381 acgattgcgc gcgggccgca gggcttccat ccgccctccc aatcggtcgc aggaatacac
     1441 atcagcgaat aagtcagggt tccccggcct tttcctccgt tgatcacgca ctacaggggt
     1501 tccagaagat gctttcctct gccaatggcg actccgttat gaaaacactt cccggcgacg
     1561 aaatccgtca ggtgcaatgg cggttttctg accggttcga cctccagatg ctcgttcaat
     1621 cggcccgttc cgtggctcga ggtctcgtgg cgcggctcgt ggcctcaggg gcgcgtaaca
     1681 cgcacgaatg gacggaagaa aaggctcaga tgctcacggc cttcgacgaa gccgggatca
     1741 cttccgtctt catggatccg gaggatggcg gatacatcga aggaccgaaa aacttcgctc
     1801 tggccctgct ggcattcgaa ctggcgtggg tcgacggagg ggcggccacc gggagcctcg
     1861 ccgggaatct ggcgctggag ccgataaagg aaaagggaac ggaacaacag agatcgcatt
     1921 atatgaagct gtgcgttcct cctgcccccg gtgatacgcg aaaagcgtgg cgcggcgcat
     1981 ttgcgttgac cgagccaatt ccgtacgtcg gcgtagatac cggagtgctg ggcggaaagg
     2041 ttggaatcgc caggtggaat gatggagagg agccgattct ccatgtcgag aaacgagggc
     2101 gcttcatcac caacatgggt tttgccaact tcgtgtgtgc ggcggtcgat tctgacgacc
     2161 cgcgcataaa aggctcctgc atggttattc tcgaagaagg tgacccgggc gttttcgaca
     2221 gaggtacccc gacaaagaag ctggtgcacc agctttcgtc caccaccgac cccgtattca
     2281 gtctcgatat ccccgccagc aggatcatcg ggggatactc cgtgaaagac ggcgtgatcg
     2341 ttccccagtg gaatcacagc cagatcatcg aatcagtgtt ccggcgtaca cgcgtgacag
     2401 tgggcctgat gacctccgcg aagctgcttt ccgcgatcga gccggtcata cggtaccaac
     2461 gtggtcgctt ccgcggagga gcttcggggg ccgaagggac tccgcgacac gatctcggcc
     2521 tgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggccgg gattccaccg
     2641 gggtagcttc tctcccggat gacacactgg tccggttcgc catcacggac gccatcacca
     2701 acgtgctgtg ccccgcaaca aaactgtgga acaccggcca cggtgcgaac atgatgagag
     2761 aagcagtcag cctgatgggc gggtacggca tcacggaaga ctgtcccggg tttctcggca
     2821 tgaagtggat ggatgcacag ctcgaggcaa cgtacgaagg cccggaggcg gttcagcgac
     2881 gccagttgag cgtgaccatg gccaacgagc tgtttcttac catttttcgc gggtggatac
     2941 gcgaaatgcg cggtatcgcc tccgaatacc ccgaaacagg ggcgtgcacc ctggcgtcag
     3001 caatggaact gtggctctgg tcgctgacct acctgttgga agggaaagac gccgacggga
     3061 ggcctttgtt caaggatcag cggcaaggcg tcacgttccc catggcggat gcgctgagct
     3121 ggcttctggc gagccgttgc cagatactcg acaccatcga gctggccgcc agaggggcag
     3181 aacgtggact ggacgcggag gcattacccg ggtacgtccg atttttcagc gacctctgcc
     3241 acgttcaggc tgcccgggcg gcgggcgaga catcgcgcgt atgttccctg ctggtgtatg
     3301 ggtacaaccg gcatccgggg tggaaagatg agggtgcatg cgtgaccgat gaggaactcg
     3361 taagcgccga gtctctcatt ccgggttctg cggccggggt cgccggaact gcggactcaa
     3421 tcggtgctga cggtacgcgt gcaaccaaag cggggccgtg tgcgggtcat gaggggcttg
     3481 agacgtttca gcggctgcag aagcgcctgc atgcctgcct ttctggttcc gcgctcgcca
     3541 aggacagggc ggccagggcg ctcacgagcg tttccattcc cgaagcgctc gactaccctg
     3601 cctgaagagt ggtgttgtgg cgcggatttg agggtgattg gaagaaaacg gaatgggacc
     3661 cgatttggaa aaagggacag agaacgcggt cgaccgtttg aatcttgaag tggatatcgc
     3721 gtgtgtaggg ttcggccctg ccatgggcgg gttcttgacc accctcgctc gcgagatgga
     3781 acaggcatcg ggtgaaccgc acgttcagag cacggttctg cccggaatgc cgcttcaggt
     3841 agtctgctac gagcgtgccg acgacgtcaa tttcggtgtg tccggactgg tgacggaagc
     3901 cagagccatc agaaacacat tccccgaaat gaattcctcc gagataccgt tggctcatcc
     3961 ggtctccgag gaaaaaatcg tgtatctact cgacccgcat ggcgcgagca ggcggtcgct
     4021 gggtttgaga attgctgaca gcgcaattcg gggtctccgg tgggtactgc catatcgccg
     4081 gtcggcattg gaattgcctt tcactccgcc gtttctcaag aaacatggcg gcctggtcat
     4141 gtcgatcggg caattcatgc agtgggtgag taaccaggtc tcgtcctcag gaatggtcca
     4201 gatatggccg ggaacacccg ttggagagcc gatcctcgaa gggaaacagg ttgtcggtgt
     4261 gcgtattgcg gatcagggcg ttcagaagga tggcacgcct gacgtcggct acatgccggg
     4321 aatggatgtt cgtgcgcgcc tcaccgtcgt cggcgacggt ccggtcggtg cggtggggca
     4381 acgtcttgac gagcagttcg ggctcccgga aggccaccat cagcacgatt gggcggtcgg
     4441 catgaaggtg gtcgtgagct tgcccgacca ttgtgaccta gtcccgggaa cggtcttgca
     4501 cactctggga tacccggaac ccgagatctt cggttttctt tacgttcacc cggaacgcat
     4561 cgcgtcgctc ggcattttcg ttccctctac tctcgcgagt cccgtccgga cggtataccg
     4621 ctatctgcag cactggatgc tccaccctta cctctggcat cacctcgaag gcggcacgtt
     4681 gaggtcatgg ggcgcgaaaa cgcttcagga atctggtcgc ctcggagagc cgtacctggc
     4741 aggcgacgga tacgcccgaa tcggagaggg atccggcagt acgaacgttc ttacgggatc
     4801 cggtgtggat gaggcatgga tgaccggtgt cctgcttgcc gaaggcgtca tcgatatgct
     4861 gaaaaagggg gaacccttta ctcaggcgaa cctcgacgct gcatatgtta accggcggag
     4921 gaaaagctgg gtggaaacag gctcgcgcaa agcccaacac gccagagatg gcttccggaa
     4981 gggcttcgtg gccggtgtcc tcggcatggc tgtttcgggt cttacgcgcg gcgcgctgaa
     5041 cctgaaaaca cgatcggcac atcctcgcga aggcatacca gacctgaacc agttttacag
     5101 ggaccggatt ccgagtgaag agcttcagcg aatacgcgaa gagtgcgcga aaaacgccat
     5161 tccgctgcat gacgcgctga tgacaagggc ggggtggcct gaaattcctc tggacggcaa
     5221 actgcttgtt tctcagcagg atgcgcttct cctcggcggc aaagttcagg cagcgccggg
     5281 gtttgctgac cacgtcatgt ttgtcgatcc ggcagtgtgt gaacagtgcg ggaccaggct
     5341 gtgcatcgaa gcctgttctg cgcaggcaat tgctccgtca ccttcaggcg gaactccgga
     5401 gtttgatcgc gagaaatgca tccactgtgg cgcgtgcatc tggaactgca cgcagcctcg
     5461 gccgggagac agggaacgcg gcaatatccg gttttatgcc ggggcaggcg gcctgcattc
     5521 agcggaaaat tgactttttc agaaggaaca ggagttcatg gaatctggat atcacatcgt
     5581 tgtatgcgga agtctggtgc cggatccgtt gcagaccctc gagccagatg tcggaccgaa
     5641 aggacccgcg cttaagaacg agatggtcct gccatccgtg ctggatccgt gggcggccca
     5701 cgcgctgtac gaggcggcgc atctcgcgaa agaatacgcg ggatcgaaag tgtggctggt
     5761 cagtgttggt ccgaagcagc gactgcagca gctcatgatg actgtcgcgc agaaagctcc
     5821 gttcagcttc gtgccggttg atgggtctgc cagcggcttc tgcgacccgt ttgaaaccgc
     5881 aaccgcgctt gcgaatgcaa taggtgcgat ccccgacctg gacaggagcc ggctgcttct
     5941 cttcggaggg tacgcgtctg cgtcgcggga cgcgggcgta acccttcagg ttgtaggcga
     6001 aatgctcggg attacggagg tctttctcgc ggttgattcg ctgtcggttt ctgagaacgg
     6061 cgaaatcagt cttctcgaac gcgtggaagg cggcaattac ctcttcagcg agtgcgattg
     6121 tcccccgctc gttgtcggct gggcgacagg ccacctgcct gagccgccga acaacccgca
     6181 aatcgggatg gcgaacatgc gaagcatcat gcctgctctg cagaaggcgc cgcaggtgca
     6241 ggttggtgcg gaaggggtat cgtttgcgaa ggtggaactc ccgcagcagc aacgaaccac
     6301 gcgcatcgtc aaggatgtgc cggtggaaca gattgccggg gaaattgtcg cgtggatacg
     6361 gggatgactg agccagtgag gaacgatagc gcaatgaaaa agatccttct tctcgcattt
     6421 gttgaggaaa atggggcgct cgggaaacct gcgcttgaag cgttatccgg cgcgtgcgag
     6481 cttgcacagg cattcggtgc ggcactgaac gtcggattga ttggcgagtt tgctcagaaa
     6541 gccgcggaca gcatcgcttc gtgtggcgca aaccaaattc tcgccgtcca gggaccggag
     6601 ttcgcgtctg caaggtgtgc aaccgattca gcggcggctg tggccttgat tcgcgctgcc
     6661 gaagcggatt tcgtcctggc gccgcaaaca acccgttcgg cgcgttgcat gcctggtgct
     6721 gcgtttcggg cgaagggccg catcgacacg cgcataaccg ggctccgcgt ggacgaaggt
     6781 acactcctca tccagcgctg gtactatcgc cagaggatgg tcgctgagtt gacacggtct
     6841 cagcgaccgt gggtggtgct gctcgaggcg ggggtgttca acccttggag cgagggcagc
     6901 gggcacgcgg cggtctccat cgtacctgtg gaaccggtgg aaacacgtac ccggatcgcc
     6961 ggcatcgaac cggcatccgc gggaacccag acgattcgcc ccgatgcgga cctcctgttc
     7021 gttgcagggg ccggttggac gaaaaagcag accgacgggg aagttcatgt cgaagaagcc
     7081 gagaacctga tccggagctt ccttgacctg actcacgcat ccctcgggag cagcaaatcc
     7141 ctggtggatc agtccgggga agggcagaag gtgatcagcc ttctgactca catgcatcag
     7201 gttggtcaaa cggggtccac gccgcgccat cccaaagggc ttgcgacctg ttgccacggt
     7261 gaggaaccgc acgtggtggg atggcgattc atcaacgaac ggcgcgccgt gaatctcgat
     7321 cctaattgcg ggtgggcgag ggggaaggcg gacgttctct acgtcgctga cgcattcgct
     7381 gtcatgggga aagtgatcga actgctcgga aaggatactg gacaggcggc agtctgacag
     7441 aaccggcttc gagttcggtt tctgaactcg agctcacctg ccccgccaga aggtctgaag
     7501 gccgtccaac gctccggcga cttcgcgttc cgtgaatttc acctggatcc gcatgaaagg
     7561 cccctgtccc cagtatgaca ggcccgcgaa gtcgctgtcg tggtaaccga cgaagttgta
     7621 cccgagcgct attcgtgcat tgccaccaag aacacggccc acttccgccg aagctccgtg
     7681 ctgcagatca ttggccgtgt gctggctgat caagcggtac tccccgagca catcccaagt
     7741 ttcccgcaat tcgtagcgga tgcttcccat ccacaggtct gtgagcgacc gcgaggacac
     7801 tccctcgctg gaaagttggg ccaccttgaa cgcgtaccgg ccgaacacct caagctgaga
     7861 gcgcggctcc agcatacctt ccagcgatcc gatgtgtact gaacgatccg tggccggcgt
     7921 caacatcccg ttatactgct tcttgtactc gtatttaccg aggacgttca gataatcgct
     7981 acggtccgac cgccatgcga gaccggacag gaaatgatgg cttcgcaacc ggttccatgt
     8041 acccgtggcg aacgtcgctt cgcccgcata cgtgtgtttc gcaagcaggg aaaggttccg
     8101 tgccagcaca acgtcgaacg cccccgaaac caccattttg tcaagcgtct gaccttcccg
     8161 ctgttcgaag cgggcgctgg ccttgaataa ttccggaggc aggtactccc cggcgatgct
     8221 gtacgagtag aaatcggcgg agcgattgcc tctcagcgtc tgcatccgct cgaagcctgt
     8281 gctcatggat acgtacggga acggcatgaa ccggtgccgc aaaccgacga tcgcttcgtt
     8341 cttctgaccg ttgatcccgc cgtccagttt gtaattggcg tatgccgtcg tgttgtccgt
     8401 tacacgggat tgcaggccga tggcggtgta gctggaatcc acgaaattgt agtcgcggaa
     8461 cgcatgctcc gctatcacgt ccagccgcgc aagaggtgac cagcgcgcct gaagccggtt
     8521 cagtgttgga cggtacgtaa cgtcttcgtc aaggaagttc tgatcccgtt ccgccttgaa
     8581 agtgagtgtt cgcgacgccg cccattcgag tccggtgctg accaccccgg tgcgggtttc
     8641 ctgacctgct ctgtcaacgt tcgtgatttc cgtggacgca agccccgtga gtgtgcgcca
     8701 gcggtacgtt ccacccagtg cggcgctgtt ccgaacctcg cgattcacga cgtcgtcgga
     8761 acggaacgct tcccccgtaa gactggcgaa ggcgaccggc gaccacgtaa cctttccgcg
     8821 cattttcgtc acaccgggaa gggcggaagt gctgctcgga ttatagaacg cacgctcggc
     8881 gcttctgtag gaaaggtcat aattcagagt gcttcgatac cgtccctgcg cgccgaattt
     8941 ccatgcccat cccgtctgcg ccagatccga ccttgccagt tccgcattga cgaacacgtt
     9001 ctggaatggc cggtaacctg cgtcaatgcc gctcaaccag taatcgcccg tggagcgtcc
     9061 ttcgcccaca agcgaagtgc cgacttccca tctgtcgccg gaatgaagcg cgaaacgacc
     9121 gccaccaacc gtgtgccgct gaagcgcgcg tgacgcttcg taggtggcga cgatgaaaat
     9181 cgggttctcg tcaaaacccc ggctcgggat tggtttcttg aacagaatcg taccggcgag
     9241 gtagtctatc tcatagtcga cgaagcgata ctgcacttct tcgcgcagga tgttttctgg
     9301 atggatgcgg tcgcgcgtct gaatgacgat gcgctcgctt ccttccacca cgcggacgcc
     9361 acgacgcgcc gcggacaggt aatagttccc cgaaacacct tcaccaggga tctcgtcaac
     9421 ctgtatcgcc tgttccgtga acgcaccaaa cagactccac gcggtgtgtc tgttgcgcat
     9481 cgccgcggcc acacctgtga acgtccgcct gtatgccgta agttctgcct gcgacagatc
     9541 gggagcaaaa tcaccgtact gcaggtagtt ccgttccttt gcgagccgga agaagaactt
     9601 gctcgcggaa ggcgcttcgt agaaaatgga actggcgtcg ccatggatgg gataggctcg
     9661 ttctggcgtg agataacgat agacctggtc atcaaaccgg cggtcgctgt cgaacgaggt
     9721 cgtcaggagg tacccatttc ggaccgtccc gcggccaaag aatgcggcct tgccttttcc
     9781 atacgcgccg ctgttgaagt cctttccgaa attcacaccg gcaggcggag cggcgttcgt
     9841 cttccatcca atctggcctg atgctatacc gaccagcaca aatctttcgt gcggcacggt
     9901 gtagttgaga gtcgcccgcg cgacgatgtt cgacgcgttc gccgtcagtg ttgcctcgcc
     9961 cggtttcgcc gaactcagga tcaatgcaga agcgtacccg ccacgcatct gaacctgcac
    10021 tccgcgttcc tcggggtaga ggtccggcgt gatgatcgaa ccgttgtcga tctcaaaggt
    10081 gacgacctgt ccatccgcca gcggcatacc ccactcgtcc agaacctgcg cgatggcttc
    10141 cgcctgggag aggctgtcgg cgggaagcgt ggacggggag acgtcaagca ttaccctggc
    10201 agccgggccg accacgtgga tgaagatgct gtcggcgaac acgcgaccgc ccgggaacgt
    10261 ctgagtgacg gtgagggtca ccggtcctga cggcgcggcg atgttcagga aatctacccg
    10321 cgaatcgcca cggaccgaga gagtagacac ttcccggccg ttcagccgca gggtcatcgg
    10381 catgccagtc tgcccgcgcg ctgtcagcgt cagccgatcc cgataactca ccgtgctgtc
    10441 cgccgtcgga tactctatcc atacaagttg accgggtcgc tgcacgcgct ccttcggtgt
    10501 gggcagccgt cgaagttcga ctgcgggcgc ctgagccggc gaaatgctgt ctgcaacggt
    10561 gctcgcggcc gctggctgcg cacctgcgcc gggaagtgcg ggtggctgtc ccgccgccgc
    10621 cggaaccgaa accgacgggg aaacagaacc accacctgca ggagcaacgg aaggcgcctg
    10681 caccgttcga accgcgccgt cctgtggaat cagcgaagtg ctggaatcaa ctgccgccgg
    10741 aacccggata cttccgtccc atgtctcgcg aatcgttcca tcactctgtt cgctcgtgcg
    10801 ccgcaacggg atggaacccc acgtgcggtg gctcacttca ggcgcgggca aatccgtgaa
    10861 cacgccgcgt tcccgtgcga tcttggccca caccgagtcc ccgtgctgca cgatgcgatc
    10921 tcgcaacgaa tccggtgcag gacgcgtaat gtcctccgga agcggttgcg agatcgtgaa
    10981 cgtatcgcgt tccgcatttc cagacgtgcg gatcgcgagc ctcacgcggg cggttccggc
    11041 cgggatcgtt cgcgcctcgg tcccgtaatc gaggttgatt tcaacgcggc ggttcttctg
    11101 catcccttcg ggcgtggtgt tgggcgctat tgggcggtcg ggaccgtacc cgaatatcgc
    11161 gatgtgcgtc gaatcaacgc cgttctgcac catccagttg cgcacggtac gcgcacgcgc
    11221 cagcgaaaga accatgttgt tcgggaactg ccgtgtgcgg atcggccgcg ggtcggtgtg
    11281 cccgctcacc gtgatctgcc ggccctgcgc ccgttttatc ctcgggagca ggtcgttcag
    11341 caccgcgatt ccttcgggcc gcagatccgc tttcccggtg gcgaacgtca caccggactc
    11401 cagaatgatg gtttccgacg ccgggatcgt gatcgaatct ggaacgatca cgcgctccac
    11461 ggtgaccgcc acggtgagcg actccggcgg ggccagtgcg ggtggcggag gaggcggtgg
    11521 tggtggcggt ggcgccggcg gacgcggctt cacgcggaac gggaagttcg ccttcgccat
    11581 ccccgaggcg gacacgctta cgaaccgcat ccacggatcg ttggtcgagc ggacgcccag
    11641 aatcaccggc tcgaggctgt cggggatatt gtgttccaga agccgtatcg cgtggtcgcc
    11701 ggtgcggatt tccggtattg agaagcggcc catccggtcg gcgatgacgc gcgtgccgtc
    11761 ctccatcaga aggaccacac ccggcaccgg cggctctccg tcatcgtgga ctttgttgcc
    11821 gttcaggtcc acccacgcgc gaccgagtac cacctgatcc tgcgtgaaca gtgccggttg
    11881 aacgaacaca cgcgccgttg ccggtcctgc ggaaagcgta gctccgcgcg acgtggaagc
    11941 ggttgccgtg gcggtgttct gtccgtcgcc cgcgccggcg ccgattccca gcacgaggcg
    12001 gtacgtcagc aggcgtgttt tcccggcgcc gatgccggca acgttccaga tgatgttccg
    12061 atcgcgcagc gtatcaggtg gcgcggcaac accgtccagc cgcgcggtgt tcggcacata
    12121 ctcgaaaccg aacggcatca cgtcccgcac cacgatgttc gacaggctgt cggcagtgct
    12181 caggttctcc agcgcgattc ggtagaccac aaaatcgccc ggctccgcgg cggtgatgtc
    12241 cgatgtcttc gtaatgcgga agtagggcac cgtcacgagg tcatccgcgc ggttcgagct
    12301 gatcttgaac gccccgacgc tcgcggtcgc ggtgttgggg atcgggtaat cgcccggcgt
    12361 caggtcgcca cgcggttccg tcgcgaaggt gaacgtccgc acggtgtcac tcgtcgcgcc
    12421 ggagacctgc cacgtcagcg tgtgcgtgcc ggcatcgtag gtggcggcgg gaacgctgct
    12481 tcccgcgata tacacaagct ccgccggcag cggatcctcc acggcgatct tgcctgcgta
    12541 ccccggcggc gttttcaggc ggatttcgta atggatttct ttcccgacaa gcgccggcgg
    12601 cgtcacaact gccttcgtga tggaaatcga ggacgcgctg cgcgaaacca catccgcctg
    12661 cgaggacacc ggcagcccac ccgtgaactg cgcccacgcg gcgtttgtca gaatcacacc
    12721 ctgatccgcc gcctcagcgc ggacgtggag cgtatcgctg gcgccgggtt gcaggtcgcc
    12781 gacattccag gtcaggacgc cgccattgga cgtcgtcgca tcggaagaaa ccagcgtcca
    12841 cccgctgttc agggtgtcgg tcaccgtcac ggacggcgcg acatcgttcc ccacgttgac
    12901 gaccacgatg gcgaagtcca ctgtttcacc gtagctcgtc gtatcggtgg aggcgagctt
    12961 cgaaaacttc agcaccggcg tcttcgggaa catcggaatg ttgtacgcaa ccgtctcgat
    13021 atcaggaacc gtcaccggac cgttggtgag cccgaacgag ttcggccgcg cgatctgcag
    13081 cccatccccc gaggacaccg tcatcatgat cgtcgcggac ccgtccgccg tcggtcgaac
    13141 ggtgaggtgc atcctgcgcg gcggatacgt cgtgccggac ggcgggtcca ccgtcatgat
    13201 gtaccgcgcc gggttcgcca ccgtgccgac ctgcgacgaa ctgaacagga acgcataccg
    13261 gccatccgca ggggttacga acgggttgat gttgttcacg ttcggcggca cgccggtccc
    13321 gttgagaagc ggaatcgcca gcacgcgttc atcaaggttc cgcagcgtca ctgtgctgcc
    13381 cgggatgagc gccgtggtga cggcatcgaa cacccgcccg aacgggtcaa tcagcacatc
    13441 ggcgttcagc accacttcgc caaggttatt cgtgcgcgac gacaccggca ccgtgaagac
    13501 gtccccgtct tccatcttca cgcgcaggga gtaggctccg ggaagcaggt tctggaagtc
    13561 gtaccggccc atggcgtcga gtaccgtggt cgcaaccacg ttcccgttcg catccaggag
    13621 ctcaaccgtc aggccggcat accgtgcgcc cgattcctca ttgcgcgcat gcaccatacc
    13681 ggcgacggca ttccgtggcg gtgatgtgga cccgtccatg ttgacgacga tcgtctgcag
    13741 aacgcccacc atcacgtccg cgccggtcac ccggtcggtc acgtgcacgt ggagctgata
    13801 cgtccccggc ggaatgtcag gaaccacgaa cacaccgttt tcgtcgagtt caacgaatat
    13861 cgagatgggg tcgcctgtta ccgtggttcc cacaagctcc acctcaacgc ggagcgccgg
    13921 gtcattcgcc cgcacctcca cattccgggt ctggtcgtac acacgcaccg caacggcgcc
    13981 cggcaggatg atgagcttga tgacgtccga atcaaatccg cggaacgacg ccaccacccg
    14041 cgcggtgtcg ttcgccgcca gaatcggggc ggtaaggatc gtcaccgctt ccccgttgcc
    14101 gcccgatacc ggcacttcga tccgttgcgt gtcgttcgag aacttgccct tgtccgccgt
    14161 gaagacgaca atcgtcccat ccggcaacgg ccggtcaaga tcgtccgtca cccgtgccgt
    14221 cagcctcgac tgatccgttc cgttcgcgac aatggtgcgc ggctccggcg taagtttgat
    14281 ccacgtgggg agaaccgttg tctgacccgt caccaccgcc gtgccgccag cggaggtggc
    14341 gttgtcgttg tacgaaatct gaagcacatc ccccggttgc gcaaacagcg tcccgtcatg
    14401 gtcagggcct gcgctctgac cgtaataggt gggcagggaa ccggaaaaca cgccggttcc
    14461 ctgcccggtt tcgtacaaca ccagggtctc aacctcgccc gtgcgcgggt tcgtcacggt
    14521 cacgtgcacc gtgtcaatcg cgttggggag gtagttccgg tcggggtcgg taagaagaac
    14581 cgtaatattc tcaccggcga cgatctgtgt ggtctgggaa atcgtaccgt ccgttccgac
    14641 gacactcccg agcaccggcg taacaacagt gttactcagg agggagaact ggaagccgcc
    14701 gttgtcgaaa tacgtgaggg aggcctgact ggtgatcagt gttcccggcg gcgtaacggc
    14761 gtacacactg gtcgctgcca cgcacatggc ggcgaccagt aggtacaccc gttgccgaag
    14821 acgcgagaac atctatcagt cccgattctg ctttttgtcg tcagtgcgcc cctctcccgc
    14881 ccgaaggacg ggagaggggc atgaacatgc tcagttcaat tcacgcgtcg tgcgtcagtc
    14941 aatcttgacg tcgaacgtga aggtgttgct gctgcccggg cccatcgcgg tgaagcgaac
    15001 ctggatggcg ccgccctcga atctcgcgcc gtcaccgtca atcgcgtcgg tgcgtgcgat
    15061 tccgttcact ttgatcgttc cggcctgata cgtcgtgttg gtcggaatca tgtccacgat
    15121 ctgcagggcg gttgccgtgc cgtgaccgtt gttcgtcacg acgaccgtgt acgtgagcac
    15181 ggtccccgga ggctgcgctc ccgtcgggga aaccgacttg acgatggaca gaacgggccc
    15241 atgaacggtc gtggtcagtg tcaccgtacc ggtcaccgtc ggatcaacac tcgatgcaat
    15301 cgttacggtg gttgcatcga ccgtcatgtc caccgtacca ggggtcacca ccaccgccac
    15361 gatgatgtgg gcaacggtgc cggccgggat gcttcccgta tccaccttgc cgtcgccgtc
    15421 ggtgtccgtc aacagcacgt caccgtcgtc gccggcgatt ccgttgttgt tggcatccag
    15481 ccagatctgg ttggtgaagc cctgcgtgga cgacgtggtg aagtcgaata catcattcga
    15541 gttgccgccg ttcgtcacgc tgaagcggta caacaacgtg tcgcccggtt ggccggtgag
    15601 cgccagcgag gtgttggaaa gcgtgacgta cggcttcgcc gccacggtga tgtcacccgg
    15661 cgtactgctc actgccggaa gtagagggcc acccgatgtg gagctgtaca acaccgaggc
    15721 aacgttcgat atgctggttc ccgccgcaac tcccgcgttc acggtcgcct ggaagaaaag
    15781 ccagccttct tgaccggccg gggagtcacc ccagttgatg gttaccgtgt ttgccgcggt
    15841 gattccgaaa tcagccgagt catccccggt ggcatccgtc tgcggcgtag ccgtcgcata
    15901 tgttccgccg gtgccggcga aaacgcgaat tgaacccgga acgtacgtca tgccggacgg
    15961 caccacgtcg gaaaacacga tgccgtacgc ggtgctggtg cccgtgttat tgccacggat
    16021 ggaataggtt acgacgtcac ccggcttcgg gtcggaggga gaagccgtct tcaccatgct
    16081 gagtactgcg gtcgacaccg tggaggtaaa gactcccgcg tccgacacac ccgtgttgaa
    16141 ctgggaggtg gccgtcagtg tcaccgcctg ggacgagccg tcaagcgcgc ctgtcggtac
    16201 attcacgacg acgaacacct ttttcgagct gtccgccgga aggctcgcga tggtgctgac
    16261 gatgttcccc ggcaccattt cgcccgcgtc gagtataccg ttgccgttcg ggtcgccgta
    16321 gatcacggcg gtccagccgg acgacagggc tg
//
LOCUS       DAOTNG010000027        15762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3708_length_15762_cov_4.690329, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000027 DAOTNG010000000
VERSION     DAOTNG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3708_length_15762_cov_4.690329"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1211
                     /locus_tag="P5179_02410"
     CDS             135..1211
                     /locus_tag="P5179_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94102.1"
                     /translation="MGAVRIGVLGGGGILGSHAPAYSKIRDRCEVVAVAEPDATRHDR
                     VKALLGADVRLYADYRDVIGLEDVDAVDIVLPHYLHLPATLCAAEKRKHVLVEKVMAR
                     NVCECDQMIDACDKAGVSLTVCHDRRYDAKWIALKEVVDSGALGEIFYWKLEHNQNVV
                     FPEGSWVRSREGLGGGAIMSCLTHQIDSLRWYGGEFARVSCMTRVIPERMEGETVGVI
                     IGKMKSGALAQLSINWFTRSNGARPNDLWYELVHVCGTRGEAYFMSSRGTFVMLHDAS
                     DAAAVSRYGREALDGFAEVPSGTWTGHERCIAEWVKSLRGEPSQVITSGRDVRGTVEV
                     AEAAYRSAQCGREIELPINPVPGE"
     gene            1264..2283
                     /locus_tag="P5179_02415"
     CDS             1264..2283
                     /locus_tag="P5179_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS94103.1"
                     /translation="MRVGVDTFTIRELKLDPFATFDYLVKRGFDGAQFGGLRSLSKEL
                     DAAMLREVHSEADRRGLYCHVSVPCCNPHIVAGNLAEHIAELTKQIEAAAEAGWHELH
                     STLGNGKERYEHPVRWTQQLADSADFVRNLGPVLRQNGSRINLETHGDVTTFELVRLI
                     EDVGPDIAGICLDTANVLCHAEDPVLAARRAAPYTHLTHTKDGIIYFSRQGYVRQGRP
                     PGQGCLDWNQILPALAEFSPDLPLSIEDHKWLFEFNVFEKRWLALHPDLTLEEFARVM
                     RLAHLCEERIACGELENPDRYEAIPYAEQMEERLAFGRDFLNALLVARGLKTKKGAEQ
                     SSAPS"
     gene            complement(2300..3031)
                     /locus_tag="P5179_02420"
     CDS             complement(2300..3031)
                     /locus_tag="P5179_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRS94104.1"
                     /translation="MGLDKRAIASSLAPYPDDLALTERVAATHPEVVAILEDFVVRRP
                     HLAYLAPTLCRVICALIRCYDRGGTLFLCGNGGSFADSVHIAGELLKSFERDRSLTSE
                     QKAKFAGLPDAEALTTHLEQGLRAYALGNSNPLATAIQNDNKLRDIHYAQDLFAMGRP
                     GDVLLGISTSGNATNVLYAVQVGKALGMTTIGMTGENGGKLAAMADIAIKAPGTVTKY
                     VQEDHFPTYHAICAMIEAYYFPKPR"
     gene            complement(3061..4065)
                     /locus_tag="P5179_02425"
     CDS             complement(3061..4065)
                     /locus_tag="P5179_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94105.1"
                     /translation="MEYRGKYPYFPYEKISTYPIKNRKNKVRQEDLYFPDRVMAMTPE
                     FSHPELGNIADTIVKARDKGYPVIWLMGAHPVKAGMSPLIIDLMQRGICTLLCGNFAS
                     AIHDFELALIGETSEVVPNALPRGEFGLAAETGFYINDAMKWGNDRKLGLGETLGRVI
                     NGEAAQELKMEFPWKHESYLAQGYLKGVPVTIHATMGTDITDQHPNFDGECKGGCSGR
                     DFGVFAAHVLEMHRKGGGVYLNVGSAVTGPEVFLKAVSMAANVGEPPTGLVTADFDIR
                     PANLEHMQDEHKHSYYFRDVKSVVTRIPEAFGGKGHYIQGDFFKAIPALYQEIRKRMG
                     "
     gene            complement(4119..4913)
                     /locus_tag="P5179_02430"
     CDS             complement(4119..4913)
                     /locus_tag="P5179_02430"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRS94106.1"
                     /translation="MSSFIAVDIGNSDVKFGLFEGRNLLQVERLETGLLTEIDTVSDL
                     SQIAPRVAELLVGQRYAVIGAVVSWAAARVASLLAANGTGVTGMTSLSGFGLRIDYEH
                     GEPGVDRVAACAEAYARKGGPLVLVGAGTALHTNVVTREGVYLGGAIMPGFRLMFEAL
                     TAGTDQIRPVSPSVPSGVIGRSTPECVQIGVYHSWLGGALRLVRRTCEEVGGHPALWL
                     TGGQAEWLKTEFPEAEFDPDLTLKGLARAFCGADQQNYRNSQNRTA"
     gene            complement(5028..6140)
                     /gene="alr"
                     /locus_tag="P5179_02435"
     CDS             complement(5028..6140)
                     /gene="alr"
                     /locus_tag="P5179_02435"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRS94107.1"
                     /translation="MAGATKATVDLEALRANVRFVRELVGKGRLILACVKANAYGHGA
                     LRCANAALQAGADALGVARLDEALELRQSGIGARIVLIGPESLEATNDLVRADVEIAI
                     DCADRLEAVLRAGKASRRVPIVHLAVDSGMARFGASFEEASQLAVRLSQAPEVSWAGV
                     MTHFPVADTDQQRSVEQWRRFHALLSGWKTRGIAVPCCHAANSAAIMGLPETHADMVR
                     PGIMLYGIPPGTAHTSDKLRPVLRLETSVVALHWREEGDYVGYGETFRCNRRTLIATI
                     PAGYGDGIPWTAGNRSWVLVNGRKAPVVGRISMDQTTIDVTDLREVHLGDPVVLIGSK
                     GDQAITANDWARWCGTISYEITTRLRGRPCEIRYIE"
     gene            complement(6178..7194)
                     /gene="tsaD"
                     /locus_tag="P5179_02440"
     CDS             complement(6178..7194)
                     /gene="tsaD"
                     /locus_tag="P5179_02440"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRS94108.1"
                     /translation="MIVLGIESSCDETAAAVFDTESGLRSNVIASQAEHIPFGGVVPE
                     MASRAHTERIVPVVRQALADAGVTLDEIHALAVTAGPGLIGSLLVGVSFAKGVAFTRN
                     LPLVPVHHIEAHVFASRIEHPDLAPPFLALVVSGGHTQLIWVPKWGTYRVLGTTRDDA
                     VGEALDKVAKILGIGYPGGPLIDRLARDGNADGVKFPKAFIKGDPFAFSFSGLKTAVL
                     LAVEKTPENELELRKADLAASFQNAAVGALVQTTLRAARKMRTKRVVLAGGVASNSAL
                     RREMEAACARIGIEVTRPSPVFCTDNAAMTACAGAFWFERGRRAGYNFNPNPRLPLDA
                     FGNE"
     gene            complement(7191..8168)
                     /locus_tag="P5179_02445"
     CDS             complement(7191..8168)
                     /locus_tag="P5179_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRS94109.1"
                     /translation="MQKTESSALMSLLEVLRRNWPLKVASFIVGFTLWLHVKTDQTYE
                     RVVSLPLEIREPSGRFVVSNDVPRTVQVRISGSGKQILVGLRHARVVLKPRVNRPEAL
                     TLDISTDDVQGLDPNRGIVVTNIEEPRSVLLDFDFLEMREAQVIPRVGIGVKPGYTVV
                     GDVRVDPPKVRLGGPRNVIGSIRTVLTDSVVLADVSSDVGELVTLELPREMGLVASPE
                     TVRVRATVQALAERRFSDIPVVVTNLPKRVNLIANPAVVAVDLTGGEHVLENVSADQI
                     SAEVDYQQSAQGGGADLPVKVSVPPGVRVKQITPEIVRLVPGQRSESVR"
     gene            complement(8276..10483)
                     /locus_tag="P5179_02450"
     CDS             complement(8276..10483)
                     /locus_tag="P5179_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94110.1"
                     /translation="MTTLPRDPHEGFTLENDVLGVRLWGPPASPALLLGASDIWDRRW
                     FAEQQPLVTLEKIRELAFADRLAEIARSPNETIYHLYQRYDFPCPKPGAQIVFLTPFG
                     TDAHAEHTGNSLQLRVTGPRKTLIVEIWVSLTRRLVVCECTQQGLEPTDLAVRVYRHR
                     DTILPGEATDPTIGGHPSAMDFAPFPMPDAFCLPIGNGGGVKQTFPPDSTFEAGFVFA
                     AAASVVGLRASLHLDTSGRGLGAPYWAEKEGRLDHGTVKRYAPINSAEGAAATLALHE
                     PGDRFCILATVATSQDSLDPIQEAANTLVEAERQGVAFLRAEQKKALDSARRARRAIA
                     TLNGQTVLNAPATILPNLRRKGGYYGDIPMCSVGPTKFCFQDAGLWHADFHLNEIRAE
                     PLLTLGRFEDVRLFADMIRDLLPQAEENAADVYGLPGAMYPLVHFPLRCRGIAHTNLT
                     WEQDIGLNGLVAKPLWLFFRYTGDTAFLRDTAYPVMRSCADFCTAYLSEGEDGHLHIV
                     PTVSPEHWGLTARFERNRDSTSALSLTKYLLDATAEAADLLHTDSQQAERWRAAAARL
                     SPYPTCVVDGEEIWTDVAGAPPIEYNIPVPLSPVFWGDDVGLDSPPEAKALAYRTLRH
                     IRVWEPHRFYLDSCVRPRLGVPSPNAPIVPESMLLSYQSLHIFPGVPPDVPVAMQNFA
                     AEGGFRVSATKTETGRIEEIIVYSACGGVCRLTDPRTGALLQYEMQPDETLRL"
     gene            complement(10503..12533)
                     /locus_tag="P5179_02455"
     CDS             complement(10503..12533)
                     /locus_tag="P5179_02455"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS94111.1"
                     /translation="MGSFRRVESLATPQELQTAKSWFDARVSESSAEVPFAFSYGGQP
                     WTSMMRQWDPAEDRSSSDAGKTRRSIRWRDRGTGLEVRCEALLYEEFPVVEWTLYLKN
                     TGVTETPVISDLMPLDTCVARGDAGSTDLSEFLLYHHAGSTCTPGDYRPFESVLAPAT
                     TKTIATSGGRSSDENFPYFNLTYPIRNGTAGMVIAIGWPGQWKAQFLRDDGIGLHISM
                     GQELTHFRLHPGEEVRTPLIAILFWLSSDSGAGVGRDRLADRWRSQNLWRRWMLAYNV
                     PTEADRQPLKPRLMACSSHQYGEMVEADEASQIMFIDRYLEERLNPDYWWMDAGWYPN
                     EWGWPNTGTWEVDTKRFPRGLRAITDHAHSRGLQTLVWFEPERVTPGTWLYTHHPEWL
                     LGKDGEQKLLDLGNPAAREWLTDHVDRILTEEGIDLYRQDFNMEPLPYWRANDTPERA
                     GISEMRHVDGLLEFWDELRRRRPGLLIDTCASGGRRNDLETLRRAVPLWRSDYILEPV
                     GQQSQTWGIALWIPYFGTGTNHEDAYGFRSVMVPNVNALWDVRRRDFDYDLVRNLVAQ
                     WRGVGELFGGDYYPLTPYSLENDAWIGWQFHRPDLEKGVLMLFRRPESPRASDRFPLW
                     GLDVEGRYRIVDLDVRERVSVLGGDELTATGVSVDIVAAPGSAMLVYERVAS"
     gene            complement(12599..13921)
                     /locus_tag="P5179_02460"
     CDS             complement(12599..13921)
                     /locus_tag="P5179_02460"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94112.1"
                     /translation="MNERVVLIGAGSVSFTRGLVADLLDAWPKGELALVDIDPAALEV
                     ATNLARKMIAARGAGWKLEASEDRRTVLKGATAVICTVGVGGRRAWEQDVFVPRKYGI
                     FQPVGDSVGPGGSSRALRMIPAMVDIAEDILDLAPDALFFNYGNPMAPVCRAIRKATG
                     APVIGLCHGVNHTAHYLAHALGVSAARCTYNAVGMNHLTWFTEFRVDGADAMPRLREI
                     AAQRVSEAKGNPAAVAELSPFSWQLFLLFGAFPAVLDRHVVEFFPQFFRDGNYYGLTL
                     GVDAFSFEETIAAGDREFAAMEKDASSPDPLPGQYHARGNGEHEQVVSIITSMREDSR
                     SVYSANLPNMGQVADLPAGAIIESPAIATTRTLLPVVQPRLNPGIPGTLATRLLWVET
                     IVDAALERKRDLFIQALVLDGFAASLDDAAAMADEFLRVHAPYLGDFD"
     gene            complement(14070..14594)
                     /locus_tag="P5179_02465"
     CDS             complement(14070..14594)
                     /locus_tag="P5179_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94113.1"
                     /translation="MKKLLVAMLLTLSMVSFAAAEGSAAGSKAAGLGIDLAVPFGDLS
                     NVAGVGYGLTGQFDYGFNQKITLIGSIGYMMFAEKNNWEGSSIPIKVGATYSLANVMP
                     GLYAIGEVGFDMLSFDWSGPFGGSSSHNELCLVPGIGWQKPMGSMTLDLSARMSLVDG
                     GSRIGIRAGVKKAL"
     gene            complement(14669..15379)
                     /locus_tag="P5179_02470"
     CDS             complement(14669..15379)
                     /locus_tag="P5179_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HRS94114.1"
                     /translation="MIRQDYLMRLLDQLAQALARVLSLRENEARGQALDFLNRESERL
                     IGIDGAMLEILSPDAIRKATASVEKGFVAARILEEISLISEEEGDHHRATAAAVKAFW
                     LYAAAAQADASAADNTFRERIAGFGDACAERGLAEGDLLSLMAGYEMLGEFGRAEDAL
                     FALVEVARRPGDLLPVGRDFYARMAEHEDAELEKGGLPRNELQDGIDGFLARVQSRLS
                     QIEIPDEAREAERAANSS"
     gene            complement(15376..>15762)
                     /locus_tag="P5179_02475"
     CDS             complement(15376..>15762)
                     /locus_tag="P5179_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRS94115.1"
                     /translation="SGMCEAGRILHHLRNNIEDNRNTIMIVGFQAENTLGRRLVDGQR
                     EVKIFGEEHTVRANVVVIEAFSAHADWKEMQEWYAPVGSSVKRTFLVHGDDEALAGMK
                     EHLQPLTGGAITVCENGQSFDLSGKT"
BASE COUNT         3140 a         4808 c         4580 g         3234 t
ORIGIN      
        1 agcagcatgc cggccgggcg caggtatgaa gtgacttcgg tggatcacgt tgctgccgac
       61 gaagcccgtt gaacgcaggc tttcgctgcc tgggtcgccg ggggatacgc atgaatctga
      121 acggagtgtg acgtatgggc gctgttcgaa tcggagttct tggagggggc ggtatccttg
      181 gatctcatgc acccgcatac tcgaaaatca gagatcgatg cgaggtggtt gcagtcgccg
      241 agcccgatgc gacacggcat gaccgtgtta aggcgctcct gggcgccgac gtcaggcttt
      301 acgcggatta ccgtgacgtg atcggtctgg aggatgttga tgcagttgat atcgtgctcc
      361 cccattatct gcatcttccc gcaaccctct gcgcggcgga gaaacgcaag cacgttcttg
      421 tggaaaaggt gatggcgagg aacgtgtgcg aatgcgacca gatgatcgac gcgtgcgata
      481 aggcaggtgt ctcgcttacc gtatgccacg accgccgcta cgacgcaaaa tggattgcct
      541 tgaaagaggt ggtggattcg ggagccctcg gggagatttt ctactggaag ctcgaacaca
      601 atcagaacgt ggtatttccg gaaggaagtt gggtccgttc acgcgagggg ctcggaggcg
      661 gcgcgattat gagctgcctg acgcaccaga tagactccct ccggtggtac ggcggcgagt
      721 tcgcacgggt gagctgcatg acgcgagtga ttcccgaaag aatggaaggc gagaccgttg
      781 gcgtcatcat cggaaaaatg aaatcaggtg cgcttgcgca gctttcaatc aactggttca
      841 cgcgatctaa cggcgcgcgt ccgaacgacc tttggtacga actggtccac gtatgcggaa
      901 cgaggggcga agcgtacttc atgtcgagcc gcggaacgtt cgtgatgctt catgacgcat
      961 ctgacgccgc cgcagtatcc agatacggtc gggaggctct ggacgggttt gctgaagttc
     1021 cgagtggaac ttggaccgga cacgaacggt gcattgcgga atgggtgaag agcctccgcg
     1081 gcgagccttc gcaggtgatt acgagtggcc gcgacgtgcg cggcaccgtt gaggtagcgg
     1141 aagcggcgta ccgctccgcg cagtgcggca gagagatcga gctccccatc aaccctgtgc
     1201 ccggcgaatg accagactgc atacggcgag taaggcgttg cctcgggatc gtggagtgag
     1261 accatgcgcg taggtgtgga cacgttcacc attcgagaac tgaaacttga tcccttcgcg
     1321 acctttgact acctggtcaa acgtggattt gacggggcgc agtttggagg actccgaagc
     1381 ctcagcaaag agcttgacgc agccatgttg cgtgaggtac acagcgaagc ggaccgccgg
     1441 gggctgtatt gccatgtctc ggtgccatgc tgcaaccctc acattgtcgc gggaaacctg
     1501 gctgaacaca tcgcggaatt gacgaagcag atcgaagccg ccgcggaagc agggtggcat
     1561 gagctccact ccaccctcgg aaacgggaag gaacgatacg agcatcccgt tcgctggaca
     1621 caacaactcg ccgattcggc ggactttgtc cgaaatctgg gcccggttct tcgccagaat
     1681 ggaagcagga tcaatctgga aacacacggg gacgttacta cgttcgaact ggtgcgcctt
     1741 atcgaagacg tggggccgga cattgcggga atatgtctcg acacggcgaa cgttctttgt
     1801 cacgcggaag accctgtgct cgcagcgcgc cgtgccgctc cctatactca tctcactcac
     1861 accaaagacg ggattatcta tttttcccgc caagggtacg tgagacaagg acgcccaccg
     1921 ggacaaggct gcctcgattg gaatcagata ctgccggcgt tggcggagtt ctcgcctgat
     1981 ctgccccttt caatcgagga ccacaaatgg ctctttgagt tcaacgtttt tgagaaacgc
     2041 tggctcgcgc tgcatccgga cctcactctc gaggagttcg cacgggtaat gcgactcgct
     2101 cacctgtgcg aggaacggat cgcctgcgga gaactggaga acccggaccg atacgaagca
     2161 attccttatg cagaacaaat ggaggaacga ctagcgttcg gcagggattt cctgaacgcg
     2221 ttgctggtgg caagaggctt gaagacgaag aaaggcgcgg aacagagctc cgcgccttct
     2281 tgatcgcctg cgccctgttt caacggggtt tcgggaagta gtacgcctca atcatggcgc
     2341 agatcgcgtg gtatgtcgga aaatgatcct cctgaacgta cttcgtcacg gtccccggcg
     2401 ccttgatggc gatgtcagcc atggccgcga gtttcccgcc attttccccg gtcattccga
     2461 tggtcgtcat accgagcgct ttgcctacct gaaccgcgta cagcacgttg gtcgcatttc
     2521 cgctcgttga gatcccgaga agcacgtccc ccgggcgtcc catcgcaaac aggtcctgag
     2581 cgtagtggat gtcgcgcagt ttgttgtcgt tttgaattgc cgtagcgagt ggattgctgt
     2641 tgccgagcgc atacgctctg aggccctgct cgagatgagt tgtcagtgcc tcggcgtcgg
     2701 gcagaccggc gaatttcgcc ttttgctcgg aagtgagaga acgatcccgt tcaaacgatt
     2761 tcaacagctc gccggcaatg tgaacggaat ccgcaaaact ccctccgttc ccacacagga
     2821 aaagtgtccc gcccctgtca taacaccgta tgagcgcgca tatgacccga caaagcgtcg
     2881 gtgcgaggta tgcgagatgc ggacgccgca caacgaagtc ctcaagaatt gccaccactt
     2941 cggggtgggt cgccgccact cgttccgtta gcgcgaggtc atccggatag ggagcgaggc
     3001 tgctcgcgat agctcgtttg tccaatccca ttttcgcctc tcaatcggtc gaaaaactga
     3061 ctagcccatg cgtttccgga tttcctgata caacgcggga atcgccttga agaagtcgcc
     3121 ctggatgtaa tgtcccttgc ccccgaaggc ctcggggatt cttgtcacaa cgctcttcac
     3181 gtctctgaag tagtaactgt gtttgtgctc atcctgcata tgttcgaggt tggcgggacg
     3241 aatgtcgaaa tcggccgtta cgagtcctgt cgggggctcg cccacgtttg ccgccatgct
     3301 gactgccttc aggaagactt ccggccccgt cacggcagaa ccgacgttga gatacactcc
     3361 gccgcctttc cggtgcattt cgaggacgtg ggcggcgaaa acgccgaagt cccgtccact
     3421 gcatccccct ttgcactcac cgtcgaaatt cgggtgctgg tcggtgatat ccgtacccat
     3481 cgtcgcatgg atggtcactg gaactccctt gagatacccc tgcgcaaggt agctctcatg
     3541 cttccacggg aattccatct tcagctcctg cgccgcttcg ccgttgatca cgcggccgag
     3601 cgtttcccca agtccaagtt tgcggtcgtt gccccatttc atcgcatcgt tgatatagaa
     3661 gcccgtttcg gcggcgagac cgaactctcc ccgtggcagc gcgttcggca cgacttccga
     3721 agtctcgccg atcagcgcca gctcgaaatc gtggatcgcg ctcgcgaaat tgccgcacag
     3781 gagggtgcag atgccccgct gcatcaggtc gatgataagg ggcgacattc ccgctttcac
     3841 cggatgagcg cccataagcc atatcaccgg gtaccccttg tcgcgcgctt tgacgatcgt
     3901 gtcggcgatg ttcccgagct ccggatgcga gaattccggt gtcatagcca tcacacggtc
     3961 ggggaaatac agatcctcct gccgaacttt gttcttccgg tttttgatcg ggtaggtgga
     4021 gatcttctca taggggaaat atggatactt gccgcgatat tccatttttg agcctccttt
     4081 gcaacgaaaa aacgacagac gatttcggcg cgatgaatct aggcagtacg attctgcgaa
     4141 ttgcggtaat tttgttgatc ggcgccacaa aaggcccgcg cgagcccttt cagcgtcaga
     4201 tcagggtcaa actcggcttc agggaactcc gttttcagcc attctgcctg tcccccggtc
     4261 agccacaagg cggggtgtcc tcccacctcc tcgcaggtcc gtcggaccag gcggagcgcc
     4321 cctcccagcc atgaatggta cacgccaatc tgtacgcatt ccggagtact ccgaccgata
     4381 actcccgaag gaacggaggg cgaaacgggg cgtatttggt ccgttcccgc ggtcaacgct
     4441 tcgaacatga gccgaaagcc cggcatgatc gccccaccca gatacacgcc ctcccgcgtg
     4501 acgacgttcg tatgcaaagc ggtgccggct cccacaagca cgagcgggcc acctttccgt
     4561 gcgtatgctt cggcgcaggc tgccactcga tctacgccgg gctcgccatg ctcatagtct
     4621 attctcagcc cgaagccgga tagcgaagtc atgcctgtaa ccccggtacc gtttgcggcc
     4681 aaaagggatg ctacacgggc cgccgcccag ctcacaaccg caccgatgac tgcatatcgt
     4741 tgcccgacaa gtagttctgc aactcggggc gcgatctgtg acaaatcact gacagtgtca
     4801 atctcggtaa gcaaacctgt ctccagtctt tccacctgga gcagattcct gccctcgaaa
     4861 aggccgaact tgacgtctga atttccgatg tcaacggcga tgaagctcga catgcaccac
     4921 ctgttgaggg actcagggac tacgtttcca tcgaattgaa ggtactttga ggcgcgtgtg
     4981 gagccaaacg gcagatatcc gatcgagaag cgcgggagag gtagtggtta ctcgatgtat
     5041 cggatttcac aaggccgacc tcgcagccgt gtggtgatct cgtagctgat cgttccgcac
     5101 caccgggccc agtcgttagc cgttatggcc tggtcgccct tggaaccgat cagcaccacg
     5161 gggtcgccga gatggacctc ccgcaggtcg gtaacgtcaa tagtggtctg gtccatgctg
     5221 attcggccca cgacaggtgc ttttctgccg tttacgagaa cccaggagcg gtttcctgcg
     5281 gtccacggga tgccgtcccc gtacccggcg gggatcgttg caatcagagt gcggcggttg
     5341 caccggaatg tctcgccgta gccgacatag tcgccttctt ctcgccagtg cagagcaacc
     5401 acgctcgttt ccaaccgaag gaccgggcgc aatttgtccg aggtgtgcgc tgttccgggc
     5461 ggaatgccgt aaagcatgat tccggggcgt accatgtccg cgtgagtctc aggtaacccc
     5521 atgattgccg cgctgttggc cgcatggcag cacggaacgg ctattccgcg ggttttccaa
     5581 ccgctgagaa gtgcgtggaa ccggcgccac tgctcaacgg acctttgttg atcagtgtcc
     5641 gcaaccggga aatgcgtcat gactccggcc caactaactt ccggcgcctg ggacaggcga
     5701 acagccagtt gactcgcttc ctcgaaagac gcaccgaaac gtgccatacc ggaatcaacc
     5761 gcgaggtgga cgatcggaac acgccgagac gctttgcccg ctcgaagcac agcttccaac
     5821 cgatcggcgc agtctattgc gatttcaacg tccgctcgta ccaggtcatt ggttgcttca
     5881 agtgattcag gtccgatcag cacaatgcgc gcgccgatgc cactttggcg caattccagc
     5941 gcttcatcga gtctcgcgac gcccagggcg tctgcgccgg cttgcagcgc tgcgttcgcg
     6001 caccggagtg ccccgtgtcc gtacgcgttc gcttttacgc acgcaagtat caaccggcct
     6061 ttgcctacga gttctctgac gaaacgcacg tttgcgcgca atgcttcaag gtccacggtg
     6121 gctttggtgg cgcctgccat tcgaacgatt cctgttgcgt tctgtgctcg ctgtgctcta
     6181 ttcgttgccg aacgcgtcga gtgggagacg ggggttcggg ttgaaattgt accccgctcg
     6241 acgcccgcgc tcaaaccaga atgcgccggc acacgcagtc atcgccgcgt tgtccgtgca
     6301 aaagaccggg gaaggcctcg taacctcaat gccgattcgg gcgcaggcgg cttccatctc
     6361 gcgacgaagc gccgagttgg aagccactcc gcctgcgagg acgacgcgtt tggtcctcat
     6421 tttccgcgcg gcccggagtg ttgtctggac aagtgcaccg accgctgcgt tctggaaact
     6481 tgcggcgagg tccgctttgc gcaattccag ttcgttctcc ggcgttttct ccactgccag
     6541 cagcacggcg gttttcagcc cgctgaaact gaacgcaaaa gggtcccctt tgatgaaagc
     6601 cttcgggaac ttcaccccgt ccgcgttacc gtcacgggct aatctgtcta tcagtggccc
     6661 cccggggtac ccgatgccga ggattttggc aaccttgtca agcgcctctc ccaccgcgtc
     6721 gtcccgcgtc gttcctagaa cgcgataggt gccccatttg ggtacccaga tcaattgtgt
     6781 atgcccacca gacacgacga gagcgagaaa gggaggcgcg aggtcaggat gctcgatgcg
     6841 gctggcgaac acgtgcgctt cgatgtgatg gacgggaacc agcgggaggt tgcgggtgaa
     6901 ggcaacaccc ttcgcgaaag acaccccgac gagcaacgaa ccgatcaaac cgggacccgc
     6961 cgtaacggcg agtgcatgaa tctcgtcgag ggtcacgcct gcatcggcga gtgcctgccg
     7021 gaccacggga acaatgcgct ccgtgtgggc ccgcgacgcc atctccggaa cgacgccccc
     7081 gaacgggatg tgctccgcct gtgaggcaat tacattagag cgcagaccgg attccgtgtc
     7141 aaacaccgct gcggctgttt catcgcacga actctcgatg cccaggacaa tcatcggaca
     7201 gattccgatc gttggcctgg cacgagtcgg acgatttccg gggtgatttg cttcacacga
     7261 acgcccggcg gaaccgacac cttcaccggc aggtctgcgc ctcctccctg cgcgctctgc
     7321 tggtaatcca cctctgcaga aatctggtcg gcggagacat tttcaaggac gtgctcacca
     7381 ccggtgaggt ccaccgccac aacggcggga ttggcgatca ggttcactct cttcggcagg
     7441 ttcgttacga ctaccggaat gtcgctgaag cgccgttctg caagcgcctg gaccgtcgcc
     7501 cgcacccgca ccgtttcagg gcttgccaca aggcccatct cgcggggcaa ctccagtgtc
     7561 accagttcgc cgacgtctga ggaaacgtcg gcgagaacca cgctgtccgt gaggacggtg
     7621 cgaatactac cgatgacgtt tcgcggcccc cccaatctta ccttcggtgg gtctacgcga
     7681 acatctccga cgaccgtgta gccgggcttt acaccgatac caaccctcgg tatcacctga
     7741 gcctcacgca tttcgaggaa atcgaagtcc agcaataccg atcgtggttc ctcgatgttt
     7801 gtgacgacaa ttccgcggtt cggatccaac ccctgtacgt cgtcggtgga tatgtccagc
     7861 gtgagtgctt cggggcgatt tactcgcggc ttcaggacga cgcgcgcgtg gcgcaacccg
     7921 accagaatct gtttccccga gcctgagatc cgtacctgca cggtccgcgg tacgtcattt
     7981 gatacgacga aacgccccga gggctccctg atttccagcg gcagcgaaac aactcgttcg
     8041 tacgtctggt cagttttcac gtgcagccac agggtgaaac cgactatgaa cgaggcgact
     8101 ttcaacggcc agttgcggcg gaggacttcg aggagactca tcagggcgga gctttcagtt
     8161 ttttgcacaa cagggacgga attccatcgt caaaccccga tgtggcgagg ccgacaatct
     8221 tgacaacatt ataggtgcgg cggcctcccg ggtcaacctg agcgccttcc cggcgtcaca
     8281 gtctgagtgt ctcgtcgggt tgcatttcgt attggagcag cgcaccggtt ctcgggtcgg
     8341 tgaggcggca taccccgcca caggcgctgt agacgatgat ttcttcaatg cggcctgttt
     8401 ccgttttcgt tgccgatacc cggaatccgc cctctgccgc gaaattctgc atcgcaaccg
     8461 gaacatccgg aggtacgccc gggaaaatgt gaaggctctg gtaggaaagg agcatgcttt
     8521 ccggaacaat cggggcgttc ggactgggaa cgccgagccg cggccttacg cacgaatcga
     8581 ggtagaaccg gtgtggttcc catacccgga tgtgacgcag tgtgcgatag gccagtgctt
     8641 tggcctcagg aggtgaatcg aggccaacat cgtctcccca gaacacaggc gaaaggggaa
     8701 ccgggatgtt gtactcaatc ggaggagcgc cggccacgtc ggtccagatc tcttccccgt
     8761 cgacaacgca cgttgggtac ggcgacagtc gcgcggctgc ggccctccat ctctcagctt
     8821 gctgagaatc ggtatgcaac aggtcggccg cctcggctgt tgcgtccaga agatacttcg
     8881 taagcgaaag tgccgatgtg ctgtctcgat tgcgctcgaa ccgcgcagtg agcccccagt
     8941 gctcgggaga caccgtcggc acaatgtgca gatggccgtc ttccccttct gaaaggtacg
     9001 cggtgcaaaa atcggcacag cttcgcatca ccgggtatgc cgtatctcgc agaaacgcgg
     9061 tgtcccccgt gtagcgaaag aaaagccaca gaggttttgc gacgagcccg ttcaacccga
     9121 tgtcctgttc ccatgtgagg ttggtgtgcg cgattccccg gcatcgaagc gggaaatgaa
     9181 cgagaggata cattgccccc gggagcccgt acacatccgc cgcgttttcc tcagcctggg
     9241 gcagcaggtc gcgaatcatg tccgcgaaaa gccgcacgtc ctcgaacctt ccgagcgtca
     9301 ggagcggctc tgccctgatt tcgttaaggt gaaaatctgc gtgccaaagc cccgcatcct
     9361 gaaagcagaa tttcgtgggc cccacgctgc acatggggat gtcaccgtag tacccaccct
     9421 ttcttcgcag gtttggcagt atcgtcgccg gcgcgttcag gacagtttga ccattcaaag
     9481 tcgcgatcgc ccgccgggct cttcgcgctg aatcaagggc ctttttctgc tccgcacgca
     9541 gaaacgccac tccctgccgc tccgcctcca cgagcgtgtt ggctgcttcc tggatcgggt
     9601 ccagggagtc ctgtgacgtg gcaaccgtag ccaggatgca aaacctgtcg ccaggctcgt
     9661 gaagggccag agtcgccgcc gcgccctcgg ctgagtttat cggggcgtag cgctttaccg
     9721 tcccgtggtc gaggcgacct tctttttctg cccagtaggg tgcgccgagt cccctcccgg
     9781 aagtgtccag atggagcgat gcgcgcagac caacaacact ggccgcagcc gcgaatacaa
     9841 aaccggcttc gaacgtgctg tcgggcggaa aggtctgctt cacgccgccg ccgttgccta
     9901 tgggcaggca aaacgcatcg ggcatgggaa acggcgcgaa gtccatggcc gacgggtgcc
     9961 ccccgattgt cggatctgtc gcctccccgg gcaggatggt atcgcggtgg cggtacaccc
    10021 gcacggccag gtccgtgggt tccaggcctt gctgcgtgca ttcgcacaca acaaggcgtc
    10081 gcgtgagcga aacccagatt tccacaatca gggttttccg cggtccggta acgcgcaatt
    10141 gaagggaatt cccggtgtgt tcggcgtgtg catcggttcc gaacggcgtg aggaaaacga
    10201 tttgtgcgcc cggcttcgga cacgggaagt cgtaccgctg atacaaatga tatatcgttt
    10261 cgttggggct tcgggcaatt tccgcgagac gatccgcgaa ggccaattcc cggatttttt
    10321 cgagcgtaac aaggggttgc tgctctgcaa accagcgtcg gtcccagata tcagacgctc
    10381 cgagcaaaag cgcaggagac gcgggtggtc cccagaggcg cactccgagg acgtcgttct
    10441 cgagagtgaa tccttcgtgc gggtctcgcg gcaaagtggt cacgtcgcag ccttttccga
    10501 acttacgaag ccacacgttc gtacacgagc attgctgacc ccggagcggc gacgatgtcg
    10561 accgaaacgc cggtcgcggt gagctcgtcc ccgcccagaa ccgaaactcg ttcgcgcacg
    10621 tcgaggtcga cgattcgata tctgccttcc acatccagac cccacagggg gaatcggtca
    10681 ctcgcacgag ggctttccgg ccgccggaag agcatcagca cccccttttc caggtcaggt
    10741 cggtggaatt gccatcctat ccacgcgtca ttctcaaggc tgtacggggt gagggggtaa
    10801 tagtcgccgc caaacagctc cccgactccc cgccactgcg caacgaggtt tctgaccaag
    10861 tcgtagtcaa aatcccggcg acgcacgtcc cacaacgcgt tgacgttcgg gaccatgacg
    10921 ctgcggaacc cgtacgcgtc ttcatgattt gttcctgttc cgaaatatgg aatccacaga
    10981 gcgatacccc acgtttgaga ctgttgtccg acgggttcaa ggatgtagtc gctcctccag
    11041 agcggaaccg cccttcgcaa cgtttccaga tcgtttcgtc gccctccaga ggcgcacgtg
    11101 tcaatcaaca gccccgggcg cctccgccgc aattcgtccc agaactcgag aaggccgtcc
    11161 acgtggcgca tttcagaaat gcctgcccgt tcgggcgtat cgttcgcgcg ccagtacggg
    11221 agcggctcca tgttgaaatc ctgtcggtac agatctatcc cttcctcggt aagtatcctg
    11281 tccacatgat cggtcagcca ttcgcgggcg gcaggatttc cgaggtcgag gagcttctgt
    11341 tctccgtcct tcccaagcag ccattctgga tggtgggtat acagccacgt gccgggcgtg
    11401 accctttccg gttcgaacca cacaagtgtc tgaaggcctc tggaatgggc gtggtcggta
    11461 atcgcgcgca acccgcgtgg aaagcgtttc gtatctactt cccacgtgcc ggtatttggc
    11521 cagccccatt cgttcgggta ccagccggcg tccatccacc agtagtccgg gttgagccgt
    11581 tcctcaagat accggtcgat gaacatgatc tgactggctt catctgcctc aaccatttcc
    11641 ccgtactggt gcgaactgca cgccatcagg cggggtttca gcggctgtct gtcggcctcg
    11701 gtcggcacgt tgtaagcaag catccaccgc cgccagagat tctgcgaccg ccatcgatcg
    11761 gcaaggcggt ccctgccgac tccagccccg gaatcactgg atagccagaa aagaatggca
    11821 atcaggggag tgcgcacctc ttcgcccggg tgcaatcgga agtgcgtcaa ctcctgcccc
    11881 atgctgatat gaagcccgat accgtcgtcc cgcaggaact gcgccttcca ttggccgggc
    11941 cagccgatgg caatcaccat tcccgcggtg ccgttccgga ttgggtacgt gaggttgaaa
    12001 tacgggaagt tctcatctga tgatctgccg cccgatgtgg cgatggtttt cgttgtcgcg
    12061 ggggccaaca ccgactcgaa cggccgatag tcgcccggcg tgcacgtgct tcccgcgtgg
    12121 tggtacaaca ggaactcgct caaatccgtc gatccggcat ctccccttgc gacgcacgta
    12181 tccagcggca tcagatccga gatgaccgga gtttcagtca caccggtgtt tttgaggtag
    12241 agcgtccact ccaccaccgg gaattcctcg tacaggagag cttcgcatcg gacctcgagc
    12301 ccggtgccgc ggtctcgcca gcgaatcgat cgcctggtct ttcccgcgtc ggaagaactc
    12361 cggtcctccg cggggtccca ctggcgcatc atagaggtcc agggctgtcc tccgtaggag
    12421 aatgcgaagg gcacttccgc ggaggattca ctgactctgg cgtcaaacca cgacttcgct
    12481 gtctgcagct cctgtggagt ggccagagat tccacacgac ggaacgaacc cattcgatct
    12541 ccctccaaaa agatgcccga tacgaggctc gatgatcgca ccaaactccc gcggttggtc
    12601 agtcaaaatc acccagatag ggggcgtgaa cccgaaggaa ttcgtccgcc attgccgccg
    12661 catcatccag cgaggcggca aatccatcca gaaccagtgc ctgtatgaac agatcgcgtt
    12721 ttctttccag cgcggcgtct acgatcgttt ccacccacag gagacgcgtc gcgagcgtgc
    12781 ccggtatccc tgggttcaaa cgtggctgta cgacggggag aagggttcgc gtggttgcga
    12841 ttgccgggct ttcgatgata gcgcccgcgg gaagatcggc aacctggccc atgttcggca
    12901 ggttggcgga gtacaccgat ctggagtcct ctcgcatgct cgtaatgatg ctcacgacct
    12961 gctcgtgttc cccgttgccc cgcgcatggt actgtccggg gagcggatcg ggcgatgatg
    13021 cgtccttctc catcgcggcg aattcccggt cgccggcggc aatcgtctcc tcgaagctga
    13081 atgcgtctac acccagtgtg aggccgtaat agttgccgtc gcgaaaaaac tgggggaaga
    13141 actccaccac gtgccggtca agaactgcgg gaaaggcgcc gaacaaaagg aacaattgcc
    13201 agctgaacgg cgacagctcg gcaactgcgg cgggatttcc tttggcttcg gaaacgcgtt
    13261 gagcggcgat ttcccgcaac cggggcatcg catctgcacc gtcaacccgg aattccgtga
    13321 accacgtcag atggttcatg ccgacggcgt tgtacgtgca acgtgcggca cttacgccga
    13381 gcgcgtgtgc aaggtaatgc gcagtatggt tcactccgtg gcacagcccg attaccgggg
    13441 cgccggtagc cttcctgatt gcccggcata ccggtgccat ggggttcccg taattgaaga
    13501 acagcgcgtc gggtgcgagg tccaggatgt cttccgcaat gtccaccatt gccggaatca
    13561 tccgcaacgc gcggctggac cctcccggtc ccaccgaatc ccccaccggt tggaaaatgc
    13621 cgtattttcg agggacaaac acgtcctgtt cccacgcacg tcgtcctccc acgcccactg
    13681 tgcagattac cgcagtggcg cccttcagca ccgtcctgcg atcctcagaa gcttcgagct
    13741 tccagcccgc acctctggct gcgatcattt tgcgcgcgag attcgtggcg acttccagcg
    13801 ctgcggggtc aatgtccacg agtgccagct cgcccttcgg ccatgcgtcg agcagatcgg
    13861 ctacaaggcc gcgcgtaaaa gagacgcttc ccgcgccgat cagaacgact cgttcgttca
    13921 tcttcaaagg actccatttt tcccggtgaa aggaaagaaa agagcgtgaa ccaagaaaaa
    13981 cgtctgacag tgggcctcag ggggcaaggg caaaaaaaga agcgggacct tttccggggt
    14041 cccgctctcc gttaaccagg agtcgagagt cagagggctt tcttgactcc cgcgcggatg
    14101 ccgatgcggc tgccaccgtc tacgaggctc atgcgagcgg agaggtcgag tgtcatgctg
    14161 cccatgggct tctgccagcc gataccggga accaggcata gttcgttgtg ggaactcgag
    14221 ccgccaaacg gcccggacca gtcaaagctc aacatgtcga agcctacctc accaattgcg
    14281 tagagccccg gcatcacgtt ggcaagggaa tacgtggctc caaccttgat cggaatactc
    14341 gatccttccc agttgttttt ttctgcgaac atcatgtagc cgatcgagcc gatgagcgtg
    14401 atcttctggt tgaacccgta atcaaactga ccggtgaggc catacccgac gccggcgacg
    14461 ttgctcagat caccgaacgg gacagcgagg tcaataccca gcccggcggc tttggagcct
    14521 gccgcagagc cttcagccgc tgcaaacgac accattgaaa gcgtgagaag catcgccacg
    14581 agaagctttt tcatgaccaa cctcctgtaa tgtgtgctgt taccttaccc ccctgcgcag
    14641 aaaaagttag cactggttct gctgcctgtc aagaagaatt cgctgccctt tccgcttccc
    14701 gcgcctcatc cggtatctct atttgactca gccggctttg gacgcgcgcg agaaaaccgt
    14761 caatcccgtc ctgcaactcg tttctcggaa ggccgccctt ttcaagttcc gcgtcttcgt
    14821 gttcggccat acgggcatag aaatccctcc ccacggggag aaggtctccg ggtcttctgg
    14881 ccacttcaac cagcgcaaac agggcatcct ccgccctgcc gaactccccg agcatttcgt
    14941 atcccgccat cagggaaagg aggtcgcctt cggccagacc ccgctccgcg caggcatccc
    15001 cgaaccctgc gattcgctcc cggaaggtgt tatcggccgc cgacgcgtcg gcctgagcgg
    15061 ccgcggcata cagccagaat gctttcaccg ccgcggctgt cgcccggtgg tggtcgccct
    15121 cttcttcaga aatgagcgag atttcctcga ggatgcgcgc cgcaacgaag cccttttcca
    15181 cggaagcagt cgccttccgg attgcgtccg gagaaaggat ttccagcatc gcaccatcga
    15241 tcccgatcag tcgctccgac tcgcggttca ggaaatcgag tgcctgaccg cgtgcctcgt
    15301 tctcgcggag cgagagcacg cgtgccagcg cctgggccaa ctggtccagc aggcgcatga
    15361 ggtaatcctg ccggatcatg tcttcccgct gagatcgaat gactggccgt tctcacaaac
    15421 ggtgattgcg cctccggtta gcggttgcag gtgctccttc atgccggcga gcgcttcgtc
    15481 atccccgtgc acgagaaacg tccttttcac ggatgagcct accggcgcat accattcctg
    15541 catctctttc cagtcggcgt gagcgctgaa tgcttcgatg actacaacat tggcgcggac
    15601 ggtgtgctcc tctccgaaaa tcttcacctc gcgctgacca tccaccagcc gtcttcccag
    15661 tgtattctcc gcctggaatc caacgatcat gatggtgttt ctgttgtcct caatattgtt
    15721 gcgaaggtgg tgcaggatgc gaccggcttc gcacattccg ga
//
LOCUS       DAOTNG010000028        15746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3714_length_15746_cov_5.510484, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000028 DAOTNG010000000
VERSION     DAOTNG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_15746_cov_5.510484"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5179_02480"
     CDS             <1..555
                     /locus_tag="P5179_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94116.1"
                     /translation="YDTTSFGAVNGNKGKINVPYRVTSGQSEVQPSLNANIVLRTPSD
                     WGMMRGDWSLSLNQTWSRGGTMNYDPFNTGNPTKLRFVDVYRTNVRLNKGLSMGSRNV
                     SFYVDVRNVFNQKTLNIAALRNQSDYIALLATMNPSTGKFDQKYKVGDSRLEDVVERR
                     FARDNDWLLYLYPREFQVGMRVNL"
     gene            611..3106
                     /locus_tag="P5179_02485"
     CDS             611..3106
                     /locus_tag="P5179_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94117.1"
                     /translation="MERTRYLRLQGWAGLGAMVGALLGLAGATTTSAAPSSPNLPNYL
                     PSSWTVSGQPMKQPGEAIDRATRGVLQHARYGRFWQPQLDNMWRVGYNGTAVWPGGRV
                     MRTGGRDNREGWGSYGTAWGGAVGCRDWDATLPTSPTDPTPVTATYPYCSNENLRYGY
                     NLDDVMLDKLCVSNSNGAANPIVGYSRLETAEISRWLPPQVIVNGKVVIPYTDTYGTT
                     PGTPAAGNGLTNYAAARRGVVDPTLRAEVVQLTAFRMAIGVDIYRTTYNYTCPPDWDY
                     LVQDFAVINTGNLDRDAAIDRPAKAINDFRFATGFEHTTSGVANGMINPNRNKDDDFI
                     ELIYPWASDATYGTRSAYLAYDGDGSGIDGPDWGDPAQTVSGTTQTMGKDLFSKAYMA
                     FAYLFAESSPGSGVDDPTQPASFTYFEENGYRLGRTRPGWDQGKAFKMIWCGHENAAT
                     DAPTKVIPPNTPTNGTAVNSSVLAVFQGLVGPQPAGWTLPAGGTYRVVAGIAAGGISD
                     RLSKDLAFATLTRDAAGQPAASRQTASEIALIQSGRDSALRTLDRMFWNVNGFVPGGR
                     YADKPAAYNQAFNVPDPPRPPACFWVSNSDRRIDLEWTREAETTPDFDTGVNDFAGYR
                     VYRAASSPDSDWVLVKDLPKASLPTTGTTVKWSDAAPSVSRDFTYFYYVVAYDDGTQN
                     WDTTPGIAKSLESGKYYTTTGWDWSPVNPGFFASPSTYPMTHLDSVIVVPNPYNSRSY
                     NPKGAQGTIDRIFFKNLPQAATIRIYTAAGSPVKTLYHFAAPGAADGASIGWDMSNDV
                     NQVVATGVYIYTVECTAGPAQGRTAVGKFIIIR"
     gene            3131..4159
                     /locus_tag="P5179_02490"
     CDS             3131..4159
                     /locus_tag="P5179_02490"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94118.1"
                     /translation="MKRTLIVGAMTCLLGLAVVNPTFAAQRGQATMTWLGISASARDE
                     AMGGTATAGDASAACAFTNPAGLGRVERGSVFFNTTSWLADMSVNDVAVAYRVPDIGT
                     FSLAFRSMDYGDFTFTTLFANEDGYVYTPSDSTGDVAGMMLGIGYGRKLTDKFSIGGQ
                     LKYVSDKLGKMNVLDRGLNIVNFGEEANQSAMLFDFGTSYHTGWKSVTINMTIQNFGR
                     SQKASNGVEEFTPPLTFKVGLQADALQFAGLETPMADLLVRVEGADPRDDRLGFNTGA
                     ELTLKPMDMVNVALRGGYSRRDQGGLTFGVGVGTNVGGFNTQLDWAYSDWGGVLGATN
                     RLGVSFAF"
     gene            4380..7475
                     /locus_tag="P5179_02495"
     CDS             4380..7475
                     /locus_tag="P5179_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94119.1"
                     /translation="MRRVHFLWIGVALLAAALLVTLYETHEVVLPKSTCVPCHGVTIF
                     TEEIMDESRGAFTGPDACEPCHAYAYHRWTKSDHCRAMMGPAPSTIAGHFSADTVEYS
                     YRDFHSAMILRDGKYFMIAPNKNGQPEEFPIHLALGIRDQQALLTKFPDGRYQILPTA
                     YDIRNQTWFESSEGIVRTTHMLRPSEPFYWTNWKRTWNYDCFGCHLSGMEKNYDPETN
                     TYNTKWRDLGIDCEACHGPGRAHARLRTVKKLWGGLVRDTTMLRLTSLSPRQQVETCG
                     ACHASKSTLALGYKPGDDWTEYYTLTLLDHDFIMPDGRYAGLIYDLIGFMQSPCYEKG
                     GLTCITCHSGHADERSIELRHYEHQNDMCRPCHADKVGNPQAHTFHPNDSPGSTCRNC
                     HMQVLPVSRMGIVDHTISIPTPENTIAFNSPNACQSENCHPNETPQWSIATLKRWHGP
                     NRIDRWTRAQVLFKGKTLDPAGVPGLIAMLADTGNNMIWRATAARMLGNIGDARAVPV
                     LRANIDAPDPMLRWQVANALGNFVYDRRVFTDLQSLLRREQHSQIRVAVASKLGYWWR
                     RDLNLTPAEKEIARQTWEQYVRQMTTAHADDPIARNTLGMVYMRRGEFDKAIREFQIS
                     LRIEPGSSGVERNLADVYTAMNRYSEALPHNQRAVDLEPDNPHLYDALSFTYLGLGRI
                     ADAEREAQRALRLDPRFAPGYVQMARIRHAQGNIAEAHRLLQKAVALDARNVEAQYLL
                     AKASQELRLRDQAVQAFVNVISLDEFSEFGYDAKNQLATMLPVGAPLPPPTATPAPER
                     PAQEGPMRPIDPHLLWPTVVYKSYAPKTTPEEVARIPEPSSFAASLDSGRAWMRLAEN
                     SRLSSATKKAYIDRSVATLSRAHRMAGSDRSKDVEVRKALGYALSVQAEHVPPANAPA
                     LLARARSVLDGVDTTALGGDAAAKASLGRSYYRLARAYNETRDYRNAIWAFERAAVLV
                     PNSFTEAISHFFVGTSYDRLGQRQEAIAAYQRSASNPHNSGRGLRCSLHGIKYPFRYF
                     R"
     gene            complement(7552..8055)
                     /locus_tag="P5179_02500"
     CDS             complement(7552..8055)
                     /locus_tag="P5179_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94120.1"
                     /translation="MMRRFLAASLLLAVALPAVAAVSVGQKGSDFSLSNVDDVRFSLA
                     TPRTTPLVLIFTTKDLGNVSQPWRDSLTTTLGSSAQIQTVLDLGDVSRFLHSVARTRI
                     RAQKSKAILDWDGSVSTAWRGNDRSRVVVMVVAPDNTVRFILSGIPVSPNVRQVTEAV
                     RRVAGTR"
     gene            complement(8247..9047)
                     /locus_tag="P5179_02505"
     CDS             complement(8247..9047)
                     /locus_tag="P5179_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS94121.1"
                     /translation="MPLSQWGRILVDEMEAAAVPPGDLWIWPLGGASLAVKSARSMVY
                     IDPYTGSPSSGVWIRMVAVPFDPRDVRIADAVLSTHDHDDHCHGETLRPFADNTGALF
                     IGPASSASKMRSFKLPETRIVSARHGDSFRFSDITVEAVEIVDHSDPTSVGWLVSVDD
                     GPVLFDGGDGMYGDYFKKVAETRRVSAATLSIAGLTGDGRKIYMDARELVAAAEDLGV
                     DLLIPKHWDLWRNVWLDPWEVVIAAQARRARFRVHIPRLGEVIRLPAR"
     gene            9327..10022
                     /locus_tag="P5179_02510"
     CDS             9327..10022
                     /locus_tag="P5179_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94122.1"
                     /translation="MLIEMRGIEKVYKLGSERVNALRGIDLDVSEGEYVALTGPSGSG
                     KSTLMHLIGCLDSPTAGRYLLAGSDVSSMSDAALARVRNRQIGFVFQTFNLLPRATAL
                     HNVEIPLIYGGFGTRERRRRATAALERVGLADRMKHQPNQMSGGQRQRVAVARALVTD
                     PSILLADEPTGNLDTATGNSILELFAQLHASGQTIVLVTHEADVAAHARRHIVLRDGK
                     IESDDANKKESAG"
     gene            10019..11449
                     /locus_tag="P5179_02515"
     CDS             10019..11449
                     /locus_tag="P5179_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS94123.1"
                     /translation="MTGIVNRRAAVLFLLAVSMAGCSMPGCNKSKEGESVDLFTTTKV
                     ERRDIEIKVTATGSIEPIRVIEIKSKASGEILALPVETGDVVSAGALLAQVDTTDVAA
                     AYRQARADLEVARVRESVSRKSLDRAADLLARGMTSSEEYDQSRLDYATARANVIRAE
                     TDLETKRERMIETIVRAPSTGTILKKNVEKGQIIASVGQVNAGTTLMTMADLSSVQVR
                     AMVDETDVGKLRPGLPVTVTVDAFPGRNFTGEIQKIEPQSVEEQNVTFFPMIVQIPNS
                     EGLLRPGMNASVDVQIMKKTGVPSLPNDAIKTLQNAGVVGMFLGLNPDSVNAFVDANK
                     AKFMDHDSTRRGPGRRGGPGPGMANAPSESSARGARPSGATARARPSRGGSMHFSSRD
                     IPQGPAPGVMAAGSVGVVFVPDSATTKVVLVKTGIQNWEYTEITSGLKEGDTVYLPPS
                     AALAMQSQAMRDRLKQFSVIPGRSSR"
     gene            11462..12667
                     /locus_tag="P5179_02520"
     CDS             11462..12667
                     /locus_tag="P5179_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS94124.1"
                     /translation="MLILEIIRVAFGALLANKGRAFLTMLGIIIGVGAVIAMMAIGAG
                     AQKAVQDRIASMGVNQLRVFADRTTIRGVTRYRSLTTDDAQALRNEMTLATAVVPDMG
                     GQYQVKYGNKNNSVRITGTTPEYFSLRSYQLAAGRFFTNGDMSASQRVAVLGSVAAEN
                     LGIGPQDVGSTVRIGGMIFDVIGIMESKGSQGGWDNPDENIFIPITTAQYRLMGNDRI
                     NGVTVQVVAQEWMLAAQGEMERILRRERQLRPEQQNDFRFFNQQELATTFAESTKIFG
                     LLLASIAAVSLIVGGIGIMNIMLVSVTERTREIGIRKALGARRSIILLQFLLESITLC
                     VLGGAIGMGLGYWVAKMMASSGGWEVIVTPQSLLLAVGFSVGVGLFFGIYPASRAAKL
                     DPIEALRYE"
     gene            12805..13902
                     /locus_tag="P5179_02525"
     CDS             12805..13902
                     /locus_tag="P5179_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94125.1"
                     /translation="MTDTMTPRERWKAAVRMEPVDRLPFWAKLPGNYPGFHGAPFSRM
                     TVDALHEWIGSDPTVHLRTGIRTVHRRASVNTVIEGDFRKTDYRIGGQCLTLVSQIDR
                     ASFSWHPIRFPVKTRDDINILADVFEDSTAEVDPDELAEAREQSRRVGERTITAQDIG
                     ESPLMVWVEWLAGIQNAHYLLNDFPADVERLFEAIHRDLVRKTEILSEHGPADVFYLT
                     ENTSTTLISPEQYRRYCARHISEYVRIVRNAGRHLILHMCGHLKALLPDLARIPVSGF
                     EAFTTPTVGNTTLAEGRRACPDKCLIGGTNAALWLEPAEAIIARIEAELDALPHHRGI
                     VVSSAGMLPQACRPETVKRVAEWVKGYRVVC"
     gene            complement(13915..14613)
                     /locus_tag="P5179_02530"
     CDS             complement(13915..14613)
                     /locus_tag="P5179_02530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS94126.1"
                     /translation="MQQFYDRYTEARLADPRHESASRWKAAMLAATYLETGELCGSHL
                     TIAEIGGGFGTVVNALGLILGSERLTVYDISAKACEEGKKAYPGTTFINAPYGERNRS
                     SWDIVVLCDVTEHVEDEDSLLRTVAEDTRFALLKIPIEKCLWRVWRRLKGKPPGFSWG
                     PDHQSGHLRGYTLWSAKRLVRRHFHVVNTSLSESVWFYGSARSLRLAIRVGKYLSVMI
                     FGGSLCVLARPRAR"
     gene            14831..>15746
                     /locus_tag="P5179_02535"
     CDS             14831..>15746
                     /locus_tag="P5179_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94127.1"
                     /translation="MAEEHEIRVRVTPRDSEQVNRVFRILTARINERYTTAVTRSGEN
                     PHLVLNVEPSLAAEAFRIREDGRATVIEGGSPLGLLYGVGKFLRTSRFEGAFTASPWR
                     GVSAPRGSLRGMYFATHFHNWYHVAPENEITRYVEDMALWGVNAIKTAFPFINLRDWE
                     DPETEHALTMTRKFARTARDLGLKFAIGINNTMFSGAPSHTRAVPLPDPTHRRGNSGH
                     PVCPSNPEGRDYLLANARRLFEELSGVGLDLVTFWPYDEGGCACDRCSPWGCNGFLRL
                     SHDFTELAREHFPNIKTVLSTWMFDTPPE"
BASE COUNT         3358 a         4604 c         4744 g         3040 t
ORIGIN      
        1 tacgacacca catcgttcgg ggcggtcaac gggaacaagg gcaagatcaa cgtcccgtac
       61 cgcgtcacgt ccgggcagtc cgaagtgcag ccgtcgttga acgcgaatat cgtcctccgt
      121 actccgtcgg actggggaat gatgaggggc gattggagcc tttcgttgaa ccagacgtgg
      181 agccgcggcg gcacgatgaa ctatgacccg ttcaacaccg gcaacccgac gaagcttcgg
      241 ttcgttgacg tgtaccgcac caacgtgcgc ctgaataagg gtctgagcat gggttcacgc
      301 aacgtgagct tctacgtgga cgtgcgcaac gtgttcaacc agaagacgct caatattgcc
      361 gcactgagga atcagagcga ctacattgct cttctggcca cgatgaaccc cagcaccggc
      421 aaattcgatc agaaatacaa agtcggtgac agcaggctcg aagatgtggt tgaacggcgg
      481 tttgcgcgag acaatgactg gctcctgtac ctgtatccgc gtgagttcca ggttgggatg
      541 cgtgtgaatc tctaacgtat agtttacgcg agcgttttct gcgaggaaca cgctcgctgt
      601 ggaggcccaa atggaaagaa cacgctatct gcgtttgcag ggctgggctg gtctgggagc
      661 aatggttggt gcgctgctgg gattggcagg cgcaacaacc acttccgcag cgccatccag
      721 cccgaatttg ccgaattacc tgccaagttc gtggacggta agcgggcaac caatgaaaca
      781 accgggcgag gcgattgacc gggcgacccg cggtgttttg cagcacgcgc ggtacggccg
      841 tttctggcag ccacagctcg acaacatgtg gcgcgtgggg tacaacggta ccgccgtctg
      901 gcccggtggc cgggttatgc gcaccggagg acgcgacaac cgggaaggtt ggggctcgta
      961 tggcaccgcg tggggcggcg cagtaggatg ccgcgattgg gatgcaacgc ttcccacttc
     1021 tcccaccgac ccaactcccg taacagcaac gtacccgtac tgctcgaacg aaaatctgcg
     1081 gtacggatac aatctggatg acgtgatgct ggacaagctt tgcgtgtcca acagcaacgg
     1141 cgccgccaat ccgattgtgg ggtactcacg attggagacc gcggagatca gccggtggct
     1201 gcccccgcag gtcatcgtta acggcaaggt agtgatcccg tacaccgaca cgtacggcac
     1261 cacgccgggt acccctgcgg ccggcaatgg tctgaccaac tacgcggcgg cgcgccgcgg
     1321 ggttgttgac cccaccctgc gtgccgaggt agtgcaactt accgcattcc gcatggcgat
     1381 cggtgtagat atctaccgca ccacgtacaa ctacacctgc ccgcctgact gggattacct
     1441 cgtgcaggat ttcgccgtta tcaacacagg gaacctggac cgcgatgcgg caattgaccg
     1501 cccggccaag gcgatcaacg atttccgttt cgcaaccggg tttgagcaca cgacctcggg
     1561 tgttgcgaac gggatgatca acccgaacag gaacaaggac gatgatttca tcgaactgat
     1621 ctacccgtgg gcttctgatg cgacgtacgg cacgcgttcg gcgtatctcg cgtacgatgg
     1681 cgacggatcg ggcatcgacg ggcctgactg gggtgacccc gcgcagaccg tgtccggaac
     1741 cacccagacc atgggcaaag atctcttttc caaagcgtac atggcgtttg cgtacctgtt
     1801 cgcggaaagc tccccgggat caggtgttga cgatccgact caacccgcat ccttcacata
     1861 tttcgaggag aacgggtatc gtctgggtcg tacgcggccc ggatgggatc agggcaaggc
     1921 tttcaagatg atctggtgcg gccatgaaaa cgccgcaaca gacgccccga ccaaagtcat
     1981 tccacccaac accccgacga acggaacagc cgtgaacagc tcagtgctcg ccgtgttcca
     2041 ggggctcgtt ggtccgcaac ctgccggttg gacgctgccc gcaggtggta cgtaccgcgt
     2101 ggttgccggc attgccgccg gtggtatcag cgaccgcctg tcgaaggacc ttgcgttcgc
     2161 cacattgacg cgtgacgcgg caggccagcc ggcagcaagt cgccagacgg catctgaaat
     2221 cgcgctgatt cagagcggac gcgattcggc gttgcgcacg cttgatcgca tgttctggaa
     2281 cgtgaacggg tttgttccag gcggaaggta tgcggacaag ccggctgcgt acaatcaggc
     2341 gttcaacgtt ccggatcctc cgcgcccacc ggcgtgcttc tgggtgtcga acagcgaccg
     2401 cagaattgac ctcgagtgga cccgcgaggc agagaccacc ccggatttcg ataccggtgt
     2461 gaacgacttc gccggttacc gggtttatcg cgcggcgtcc tcaccggata gcgactgggt
     2521 actggtaaag gacctgccaa aggccagtct gccaacaacc gggacgacgg tcaaatggag
     2581 tgatgcggcg ccgagtgtgt cgcgcgactt cacgtatttc tactacgtgg ttgcgtacga
     2641 cgacggcacc cagaactggg acacgactcc cggaatcgcg aaaagcctcg agagcggcaa
     2701 gtactacacg acaaccgggt gggactggag ccccgtgaac ccgggcttct tcgcgagccc
     2761 gtcaacgtac cccatgaccc atctggatag tgtgatcgtc gtgccgaacc cttacaactc
     2821 ccggagctac aaccccaagg gagcgcaggg aacgatagat cgcattttct tcaagaatct
     2881 gccgcaggca gccacgatcc ggatttacac cgcagcggga tcacccgtga aaacgctgta
     2941 tcactttgcg gcaccgggcg cggctgatgg cgcgagcatc ggctgggaca tgagcaacga
     3001 tgtcaaccag gtggtcgcaa ccggcgtgta catctacaca gttgagtgca ccgccggtcc
     3061 cgcgcagggt agaacggcag tgggcaagtt catcattatc cgttagcccg acaggatgga
     3121 ggaacatcag atgaagagaa ccttgattgt cggggcaatg acatgcctgc tgggccttgc
     3181 cgttgtaaac ccgactttcg cagcgcagag ggggcaggcc accatgacgt ggctgggcat
     3241 ctccgcaagc gcgcgcgatg aggcaatggg agggactgct accgcgggag acgcgagcgc
     3301 agcctgtgcg ttcaccaacc ccgccgggtt gggccgcgtt gaacgcggaa gcgttttctt
     3361 caacaccacg tcgtggcttg ccgacatgtc ggtgaacgat gtggcagttg cctatcgcgt
     3421 acctgatatc ggcacgtttt ccctcgcgtt ccgttcgatg gactacggcg atttcacgtt
     3481 caccaccctg ttcgcaaacg aggatgggta cgtgtacaca ccgagtgact cgacgggcga
     3541 tgttgccggg atgatgctgg gcatcggcta cggccggaag ttgaccgaca agttcagcat
     3601 cggcggccaa ctgaagtatg tgtccgacaa acttggcaag atgaacgtgt tggacagggg
     3661 cctcaacatc gtcaacttcg gtgaagaggc gaatcagagc gcgatgctgt tcgatttcgg
     3721 aacgagctac cacaccggat ggaagagcgt cacgatcaac atgacgattc agaacttcgg
     3781 gcgctcgcag aaggcctcga acggagttga ggagttcacc cctcccctca cgttcaaggt
     3841 gggtctccag gctgacgcgt tgcagttcgc cggcctggaa accccgatgg cggacctcct
     3901 cgttcgcgtg gaaggtgccg accctcgtga cgaccgactc ggtttcaaca cgggagctga
     3961 gcttactctc aagcctatgg acatggtcaa cgtggcgctg cggggaggtt attcccgccg
     4021 cgatcagggt ggcctgacct tcggtgtggg agtcggcacg aacgttggtg gcttcaacac
     4081 ccagcttgac tgggcgtact ccgactgggg tggcgtcctg ggtgcaacca accgattggg
     4141 agtctccttc gcattctgac agaggcgtgg aggcaatctt gcgtgggcgg agtcggcagc
     4201 gactccgccc acgtttgttg tagacacttt ccctcctgaa ttgccttctg tggctttcgt
     4261 tcggctaccc gcgtatttta agtagttgtc acctgtgctg gtttgagctg agttcgtcgt
     4321 gtcgagccga ccggtcggcg ccgcattgac cttgcgcccg acaacacacg ggagttgtta
     4381 tgcgtcgcgt tcatttcctg tggatcggag tagctctcct cgccgccgcg cttctcgtga
     4441 cgctgtacga gactcatgaa gtcgtgcttc ccaagtcgac gtgcgttccg tgccacggag
     4501 tcaccatctt cacagaagaa atcatggacg agagccgggg cgcgttcacc ggtccagacg
     4561 cgtgcgagcc gtgccatgcg tacgcctacc accggtggac gaaatccgac cattgcaggg
     4621 cgatgatggg gccggcaccc agcacgatcg ccggccattt ctcggccgac acggtggaat
     4681 actcgtaccg cgattttcac tccgcgatga ttctgcgcga cggcaagtat ttcatgattg
     4741 ccccgaacaa gaacgggcaa cctgaggagt tcccgatcca ccttgccctt ggtatccggg
     4801 accaacaggc cctcttgaca aagtttccgg acggccgtta ccagatcctc ccgaccgcgt
     4861 acgatatacg taaccagacg tggtttgaat caagcgaagg aatcgttcgt accacccaca
     4921 tgctgcgtcc gagcgagccc ttctactgga caaactggaa gcggacctgg aattacgact
     4981 gcttcggctg tcatctcagt ggcatggaga agaattacga ccccgaaacg aacacgtaca
     5041 acaccaaatg gcgagacctt ggcattgatt gtgaagcgtg ccacgggccg ggccgggcgc
     5101 acgcaaggct gcggacggtg aagaagctct ggggtggatt ggttcgtgac acgaccatgc
     5161 tccgcctgac aagtctttcc ccgcgacaac aggttgaaac gtgtggtgcc tgccacgcaa
     5221 gcaagagcac gctggcgctc gggtacaaac caggggacga ttggacggaa tactacactc
     5281 tcactctact tgaccacgat ttcatcatgc cggacggtcg ctacgcggga ctcatctacg
     5341 acctgatagg tttcatgcag agcccctgtt acgagaaagg cggtttgacc tgtattacgt
     5401 gtcacagcgg tcacgcggat gaaagatcca tcgaactccg tcactatgag catcagaacg
     5461 atatgtgccg gccgtgccac gcagacaagg tcggaaaccc gcaggcccac acgttccatc
     5521 caaacgacag ccctggcagc acctgtcgca actgccacat gcaggtcctt cctgtgagtc
     5581 ggatggggat cgtggaccat acgatttcga ttcccactcc ggaaaacacg atcgcgttca
     5641 actcaccgaa tgcctgccag agcgagaatt gtcatccgaa tgaaacgccc cagtggtcga
     5701 tcgcaactct gaaacggtgg catggtccga acagaattga ccgctggacc cgggcgcagg
     5761 tgctgttcaa agggaaaact ctggatccag cgggcgtgcc tggattgatc gcgatgcttg
     5821 cggacacggg caacaacatg atctggcgcg cgactgcggc acgcatgctt ggaaacatcg
     5881 gtgacgcgag agccgtgcct gtgttgcgtg cgaatatcga cgctcccgac ccgatgcttc
     5941 gctggcaggt cgcaaatgcg ctcggcaact tcgtgtacga tcggcgggtt ttcaccgacc
     6001 tccagagcct tctccgccgc gaacagcatt cccagatccg cgtcgctgtc gcgtccaagc
     6061 ttgggtactg gtggcggcgg gatctcaacc tgactccggc agaaaaggaa attgcccgac
     6121 agacgtggga acagtacgtg cggcagatga cgaccgccca tgcggacgat cctattgctc
     6181 gcaatacgct gggaatggtc tacatgcgtc ggggagagtt tgacaaagcg attcgggagt
     6241 tccagatttc gttgagaatc gaaccgggca gttccggtgt cgaaaggaat ctggctgacg
     6301 tgtacacggc aatgaatcgg tattcggagg cgttgccaca caaccagcgt gcagttgatt
     6361 tggaaccgga caaccctcac ctgtacgacg cgctttcatt tacgtatctg ggtctgggga
     6421 ggatcgctga cgcggaacgc gaagctcagc gggctctccg gctggatccc cgttttgcgc
     6481 cggggtatgt tcagatggcg cggatacgcc acgctcaggg caacatcgcc gaagcgcatc
     6541 ggctgcttca aaaagcagtt gctctggacg cgcgcaatgt tgaggcccaa tatctgctgg
     6601 ccaaggcgag tcaggagctg cgattgcggg accaggccgt tcaggccttc gtgaacgtga
     6661 tttccctgga tgagttttcg gagttcgggt acgacgcaaa gaaccagttg gctacgatgc
     6721 ttcccgttgg ggcccccttg ccgccgccga cggcaacgcc cgcccctgaa cggccggcgc
     6781 aggaagggcc gatgaggcca atcgacccac atctgctgtg gcctaccgtt gtgtacaaga
     6841 gctacgcacc gaaaacaacc ccggaggagg ttgctcgcat cccggagcct tcgagttttg
     6901 cggcgtctct cgattcagga cgcgcatgga tgcggctcgc ggaaaacagc cgcctttcca
     6961 gtgcgacgaa aaaagcatac atagatcgtt cggtcgcaac gctctcacgg gctcaccgga
     7021 tggcgggatc ggatcgttca aaggacgtcg aggttcgaaa agctctggga tacgctcttt
     7081 cggttcaggc ggaacacgtg cctccggcaa acgcacccgc gttactcgcc cgggcgagaa
     7141 gcgttctgga tggtgtcgac acaacggcgc tcggcgggga cgccgctgcg aaggccagcc
     7201 tcggaagatc ctattaccgt cttgccagag cgtataacga aacgcgggac tatcgaaacg
     7261 ctatctgggc gtttgagcgc gcggcagtgc tcgtaccgaa ctcattcact gaagcaattt
     7321 cgcatttctt cgtaggcacc tcgtacgacc gtctcggcca acggcaggaa gccatcgcgg
     7381 cgtaccagcg atctgcctcc aatccgcata actcgggacg tggcctgcgg tgttctctcc
     7441 atggtatcaa gtacccgttc cggtatttcc gctgaaatcc tgatccacca ccggcaaaaa
     7501 ggaacgagcg gccttttcgg gccgctcgtt ctcctcagag gaaacacctg cttatcgtgt
     7561 tccggcaacc cgcctcactg cctcagtgac ctgtcggaca ttcggagaaa cgggaatccc
     7621 actgagtatg aaacgaaccg tgttatcagg cgcgaccacc atgaccacca ctctggagcg
     7681 atcattccct ctccaggcag tcgacacaga gccgtcccag tccagaatcg ctttcgattt
     7741 ctgcgccctg atacgcgtgc gagcaacgct gtgcaggaag cgggacacgt ccccgagatc
     7801 aagtacagtc tgaatctgcg cggaactccc cagcgttgtc gtcagactgt ctctccacgg
     7861 ctggctcaca tttcccagat ccttcgtggt gaatatcaga acgagcggcg tagttcgcgg
     7921 cgtcgcaaga gagaagcgaa catcatctac gttcgagagg gaaaaatcgg aacccttctg
     7981 cccgacagag acggccgcaa cggctggaag agcaacggct aataacagag aagctgcaag
     8041 aaaacggcgc atcatgggac cctccaatcc aggtgatgtc tgaagagacg tacgcgaaga
     8101 cttcgccagg gaagcgcgtt gagtaagaaa ctcgctacca gaatatcgcg tatttctcta
     8161 aaaacagtaa gccacaggcg ccgggcatct ccgcaacgga gagagattgc cacctgggat
     8221 tcctccagac tgcttcgtcc gatgcttcag cgggccggga gccgaatgac ttctcccagg
     8281 cgggggatgt ggacgcgaaa ccgagcgcgg cgtgcctgtg cggcaatcac aacctcccag
     8341 ggatccagcc acacgttccg ccagagatcc cagtgtttgg gtatgaggag gtcaactccg
     8401 agatcttctg cggcggcaac gagttcccgc gcgtccatgt aaatttttct tccgtcgcca
     8461 gtgaggcccg caatactcaa ggttgccgca gagacacggc gcgtctccgc aaccttcttg
     8521 aagtagtcgc cgtacatccc gtcgccgccg tcgaagagca ctggtccgtc gtccacggat
     8581 accagccacc ccacggacgt cgggtcactg tgatccacga tctcgaccgc ttctacggtt
     8641 atatctgaga aacggaaaga atcaccgtgg cgggcgctca cgatccgtgt ttcgggaagt
     8701 ttgaacgagc gcattttgga cgccgaggac gcaggcccga tgaacaacgc ccccgtgttg
     8761 tccgcgaacg ggcgcaacgt ctctccgtgg cagtgatcat cgtggtcgtg cgtggacagc
     8821 accgcgtccg cgatgcgcac gtcacgcgga tcgaacggaa cggctaccat tcgaatccag
     8881 acgccgctgg agggtgatcc tgtgtacgga tcaatgtata ccatgctgcg agcggacttc
     8941 actgcaagcg aagctccccc cagtggccag atccacaagt ccccgggcgg aactgcggcg
     9001 gcctccatct cgtcaaccag aattcttccc cattgggaca gcggcattgg aatagttcct
     9061 tttgaaaagg ggcgatcgac gtggtgcctt attgtagaca ccctgggaac acttgtcaag
     9121 ccgcgcgttg gtgttcactg ccgcggcacc atgcacctcg gattcgttct gaccgggtga
     9181 tattctcgag aatctcatat gttggagata tgaaaaccgc cttcccgttc gtttaactga
     9241 aaggtattca gcaggctcgg cggcacagtg tcggctgcat tcctgcggcg ggaaacaggg
     9301 cgaagcattg gaaggggcat acgctgatgt tgatcgaaat gcgagggatc gagaaggtgt
     9361 acaaactcgg ttccgagcgc gtgaatgcat tgcgaggaat tgatcttgac gtctccgaag
     9421 gagaatacgt cgcactcacc gggccctccg ggtccggcaa gtctacgctg atgcacctca
     9481 tcggctgtct ggattcgcca acggcaggga gatacctgct cgcgggcagc gacgtgtcct
     9541 caatgagtga tgcggcgctc gcccgcgtgc gcaaccgcca gatcggtttc gtgttccaga
     9601 cgttcaatct gcttccccgt gcgaccgcgt tgcacaacgt ggagatcccg ttgatctacg
     9661 gcgggtttgg cactcgggag agacggcgcc gggcgaccgc tgcgctcgag cgtgtcggtc
     9721 tggcggatcg gatgaaacac cagccgaatc agatgtccgg agggcaacgg cagcgcgtcg
     9781 ctgtggcgcg cgctctggtg accgacccca gcatccttct cgcggatgag cccaccggca
     9841 acctcgacac tgcaaccggg aacagcatcc tggaattgtt cgcgcagttg catgcttccg
     9901 gccagacaat cgttcttgtt acgcacgaag ccgacgttgc agcgcatgcc cggaggcata
     9961 tcgtgctgcg ggatgggaaa atagagtcag acgacgccaa taaaaaggaa tcagcaggat
    10021 gactggaatc gtgaaccgcc gagcagcagt gttgttcctt ctcgccgttt caatggccgg
    10081 atgttcaatg cccggctgca acaaatcaaa agaaggtgaa tctgtagacc tttttaccac
    10141 gacgaaagtg gaacgtcgcg atatcgagat taaggtcacc gccaccggat caatcgagcc
    10201 gatccgcgta atcgagatca aatcgaaggc atcgggagag attcttgctc tgccagtcga
    10261 aaccggggac gtcgtttcgg ccggggctct gctcgcgcag gtggatacga cggacgtcgc
    10321 cgcagcatac cgacaggctc gggctgatct cgaagtggcg agagtgcggg agagcgtctc
    10381 tcgaaaaagc ctcgatcggg ccgccgatct tctcgcacgg ggaatgacgt catcggagga
    10441 atacgaccag tcccgactgg attacgcaac agcccgtgca aacgtcattc gcgcggagac
    10501 ggatcttgaa accaaacggg aaaggatgat agagacgatc gtccgcgcac cgtccacggg
    10561 aacgatcctg aagaagaacg tcgaaaaggg gcagataatc gcgtctgttg gtcaggtgaa
    10621 tgccgggaca acactgatga ctatggccga cctcagcagc gtgcaggtcc gcgcgatggt
    10681 tgacgagacc gacgtgggga aactgaggcc cggtctcccg gttaccgtca ccgtggacgc
    10741 gttccccggc aggaatttca caggggaaat tcagaagata gaaccacaat cagtcgagga
    10801 gcagaacgtc acgtttttcc cgatgatcgt tcagattccc aacagcgaag gattattgcg
    10861 accgggaatg aatgccagcg ttgacgtcca gatcatgaag aaaacaggcg ttccgtcgct
    10921 gccgaatgac gcaatcaaaa cgttgcagaa cgcaggcgtc gtcggaatgt ttctcgggct
    10981 gaacccggac agcgtgaatg ccttcgtgga cgcgaacaag gccaaattca tggatcatga
    11041 ttcgactcgt cgcggtccgg gcaggcgcgg tggccccggt ccgggcatgg cgaacgcacc
    11101 ttctgaatcg tcggcgcggg gcgcgcgtcc gtcgggtgcg acggcccggg caaggccaag
    11161 cagaggtggc agcatgcact tcagttctcg agacatcccc cagggtcctg cgccgggtgt
    11221 gatggccgcg gggagtgtcg gcgtggtctt cgtgcccgac agcgccacga cgaaagtggt
    11281 tctggtcaag acggggatac agaactggga gtacaccgag attacatcgg gactcaaaga
    11341 aggcgatacg gtataccttc ccccctcggc agcgctggcg atgcaatctc aggcgatgcg
    11401 cgaccgattg aagcaattct cagtgattcc cggcagaagc tcgcggtaga ggacgtgcac
    11461 catgctgatt ttggagataa tccgggtagc ctttggcgcg ctgctggcga acaaggggcg
    11521 ggcgttcctg acgatgctgg gcatcattat cggcgtgggc gccgtcatcg cgatgatggc
    11581 gatcggcgcg ggtgcgcaga aagcagtgca ggatcgtatc gcctccatgg gagtgaacca
    11641 gttgcgagtg tttgcagaca gaaccaccat acgcggagtc acccggtacc gttcgctgac
    11701 taccgacgac gcccaggcgt tgcggaacga gatgacgctg gccaccgcag tcgttcctga
    11761 catgggcggg caataccagg tgaagtacgg caacaagaac aacagcgttc gaatcaccgg
    11821 aaccaccccc gagtatttca gcctgcggag ttaccagctt gcggcgggca ggtttttcac
    11881 aaatggcgac atgtcggcat ctcagagagt tgcggttctg ggatctgtgg cggcagagaa
    11941 cctcggtatc gggccccagg acgtaggttc gacggtcagg atcggcggga tgattttcga
    12001 cgtgatagga atcatggaat ccaagggctc ccagggaggc tgggacaacc cggacgaaaa
    12061 catcttcatc ccgatcacca cggcgcagta cagactcatg ggcaacgatc gaatcaacgg
    12121 cgtgacggtg caggttgtcg cacaggaatg gatgctggca gcgcagggcg aaatggagag
    12181 aatcctccgc cgcgaacgcc aactgcgccc ggagcagcag aacgactttc gtttcttcaa
    12241 ccagcaggag ctggcaacaa cgttcgcgga aagcaccaag atttttggac tgctgcttgc
    12301 cagtatcgcc gcggtaagcc tcatcgtggg tggcatcggc atcatgaaca tcatgctcgt
    12361 aagcgtaacc gagagaaccc gggaaatagg tattcgaaag gctctgggag cccggcggag
    12421 cattattctc ctccagtttc tgctggaatc catcacgttg tgtgtgctcg gcggcgccat
    12481 aggcatgggc ctcggatact gggttgccaa aatgatggcc agctcgggcg gctgggaagt
    12541 catcgtaacg ccgcagtccc tgttgcttgc ggtagggttt tccgtgggtg tcggtttgtt
    12601 tttcgggatt tatcccgcca gtcgtgcggc caaactggat cctatcgagg cactgaggta
    12661 cgaatagaag cgcgaacggc agcgacgccg gggcaactgg acacgccgca cgcgtccaat
    12721 gctgtttttc cgtgccatga ccccgttcga ggtgtcccgc gggcgtcgcc cgcaccggag
    12781 gcttcccagc gaggagggta ccgcgtgacc gacacaatga ccccacgcga gcgctggaag
    12841 gccgccgttc ggatggaacc ggtggaccgc cttcccttct gggccaaact tcctggcaac
    12901 taccccgggt ttcacggtgc tcccttctcc cggatgaccg tcgacgcgct ccacgagtgg
    12961 atcggcagtg atccgacggt tcacctcagg acgggcatac gtaccgtgca ccgacgggcg
    13021 tccgtgaata ccgtaatcga gggcgatttt cgaaaaacag attaccgtat tggcggtcaa
    13081 tgcctcacac tcgtctcaca gattgaccgc gcttcctttt cctggcatcc catcagattc
    13141 ccggtcaaga cgcgcgatga catcaacatt ctggctgacg tcttcgagga ttccaccgca
    13201 gaagttgacc cggatgaact cgcggaggcg cgagagcaat cgcgacgtgt cggtgagcgg
    13261 accataacgg ctcaggacat cggtgaatcg ccgcttatgg tctgggtgga gtggcttgcc
    13321 ggaatccaga acgcccatta cctgctcaac gattttccag ccgacgtcga gcgcctgttc
    13381 gaggcaatcc accgggatct ggtgcggaag accgaaatcc tgagtgaaca tggcccggcg
    13441 gatgtcttct acctgacgga gaacacgtcc acaacactga tttctccgga gcaataccga
    13501 cggtattgcg cacgccatat cagcgagtac gtgcggattg tccgcaacgc aggccgacac
    13561 ctcatactgc atatgtgcgg gcaccttaag gctctgttac ctgacctggc acgcatcccg
    13621 gtgagcggat tcgaggcgtt taccacgccc acggtgggca acacgacgct cgcggaagga
    13681 cgtcgcgcct gtcccgacaa gtgcctgatc gggggcacga acgctgccct gtggcttgaa
    13741 ccggccgaag cgatcatagc acgaatcgaa gccgaactcg atgcgttgcc tcatcacagg
    13801 gggatagtcg tcagttccgc tggaatgctg ccgcaggcgt gcaggccgga gacggtgaag
    13861 cgcgtggcgg agtgggtgaa ggggtatcgc gtggtatgct gatctgtgcc gccgttaccg
    13921 ggcccggggt cttgcgagaa cgcagagcga tccaccgaaa atcatcaccg agaggtattt
    13981 tccaacacgt atcgccaggc gcagacttct tgcactgccg tagaaccaca ctgattccga
    14041 aagactcgtg ttgaccacgt ggaaatgcct cctcacaagc ctcttcgcgg accaaagcgt
    14101 gtaccctctc aggtgacccg actggtggtc cggcccccac gaaaaaccgg gcggtttccc
    14161 cttcagtcgt cgccatacgc gccagaggca cttttcgatc gggattttga gcaacgcgaa
    14221 cctggtgtcc tctgcgacgg tgcgcagcag gctgtcctcg tcttccacgt gctcggtcac
    14281 atcacataac acaacgatgt cccaggagga acgatttctt tcaccgtacg gggcattgat
    14341 gaacgtcgtg ccggggtagg cctttttgcc ttcttcgcac gctttggcgc tgatatcata
    14401 caccgtaagc cgttcgcttc cgagtatcag gcccagcgcg ttcaccaccg ttccgaaccc
    14461 cccgccgatt tccgcgatgg tgaggtgtga accgcacaac tccccggttt cgagatacgt
    14521 ggctgccagc atagcggctt tccaacggct ggcggattcg tggcggggat ccgccaaacg
    14581 ggcttcggtg taccgatcat agaactgctg catgcgcaaa cctcgttggc gttacgcggg
    14641 tcatttcgga tcccgcacac gccacggctt caacacgtag ttgccgtccc tttcttctga
    14701 aatgaatatc gccggtcccg aacgggccgg caacgaggga gcgtccctcc ttgactactc
    14761 cgggttgatt ccttcccttc tgtacgcagg gagggtctgg gaaaacggtt cagatgatgg
    14821 agacggtgga gtggctgaag aacacgaaat tcgcgtcagg gttacccctc gcgacagcga
    14881 acaagtcaac cgcgtatttc gcatactcac ggcgcgaatc aacgaacggt acaccaccgc
    14941 agtcacccgc agcggcgaga atcctcacct ggtactcaac gtcgagccaa gcctcgccgc
    15001 cgaggcgttc aggattcgcg aagacggtcg ggcaacagtg atcgaaggtg gctcgcctct
    15061 cggcctgctg tacggcgtgg gcaaattcct gcgcacaagc cggttcgagg gtgcgttcac
    15121 ggcctcacct tggcgtggtg tgtccgcgcc gcgaggttcg ttgcgaggaa tgtacttcgc
    15181 cactcacttc cacaactggt accacgtcgc cccggagaat gaaatcaccc gctacgtgga
    15241 agacatggcc ctctggggcg tgaacgcgat caagacggca ttcccgttca tcaatctgcg
    15301 agactgggag gacccggaaa cagaacatgc cctcacaatg acgcgcaaat tcgcgagaac
    15361 tgcccgggac cttgggctga aattcgcgat cggcatcaac aacacgatgt tcagcggcgc
    15421 gccttctcac acccgcgccg ttccgttgcc cgacccgact catcgccggg gcaacagcgg
    15481 gcatccggtt tgtccgagta acccggaggg acgcgactat ctcctggcga acgccaggcg
    15541 ccttttcgag gagctgtcgg gcgtcggcct ggacctcgtg acgttctggc catacgacga
    15601 gggcgggtgc gcgtgcgacc ggtgctcgcc ctgggggtgc aacgggtttc tccgcctttc
    15661 ccatgatttc actgaactgg cgcgggagca cttcccgaac atcaagacag ttctttcgac
    15721 gtggatgttt gatacaccac cggaag
//
LOCUS       DAOTNG010000029        15705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3724_length_15705_cov_5.134505, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000029 DAOTNG010000000
VERSION     DAOTNG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15705
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3724_length_15705_cov_5.134505"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="P5179_02540"
     CDS             complement(<1..552)
                     /locus_tag="P5179_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRS94128.1"
                     /translation="MPEQPILLVCNKHDDTMSFVDLTSYEVLETIPTGPNPHEIVVTP
                     DNRWAYLSNYAPPGNTISLIDLVAAKHVRQISTGEYSRIHGAAMAPDGRWAYFTAGQK
                     GYVVELDTRTNEVTRAILTCGKISHMVLVSPEGDFLYTANIVTKDVSVLRRTDGALAA
                     LVPCQEGVEGLGITPDGRELWALN"
     gene            complement(597..1727)
                     /locus_tag="P5179_02545"
     CDS             complement(597..1727)
                     /locus_tag="P5179_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRS94129.1"
                     /translation="MQERRTRVIYNCDADNMFIYDYPMTVDNVNRYVDEIVDTDITTF
                     FMCCNYGMDMNFRGETVQLIGDRLTAEQLEALKDPAVNKPASFQRGILNFRQLIQQGH
                     DPLGMVISRAQEKGCETFVTFRLNEVHCVDEEPDSVLLSKFWLEHPEWRVGKVGDPLP
                     DLHKTILGPVHPIVASWLPAGLDFAVPEVRDYVLSQLTEVCERYPLDGLDLDFQRFPI
                     YFRFGQEASNLPVMTEFVRSIRSMTNRVAGRRGRPILLSARIMETPEQNLGLGLDPVS
                     WAREGLIDFVTISHYLRNTHPLPIGDFRRLLPSDLPIYGSIEFEREPDRYREIARQLW
                     KDGADGIMLFNFFCTREGGAEPMFWLLNEIGRREKMLDGSNT"
     gene            complement(1740..2675)
                     /locus_tag="P5179_02550"
     CDS             complement(1740..2675)
                     /locus_tag="P5179_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS94130.1"
                     /translation="MRVCRFWEPKTGARLGIVFEETVVDATAIDPRGCADISTWLALP
                     DPIEYMRGITELAERSKNRLHIRELEHAPAPGSRHLLPPIDKQEIWGAGVTYQRMRQS
                     HVDESHGGKLFYDEVYDAARPELFFKTTPHRVAGPFGSIRVRSDSEWTIPEPELTVLL
                     SPAQRIIGFTCANDVTSRDIEGENPLYMAQSKTYSGCCALGPTILVSDDPDFARDLPI
                     SLVVIREGMAVFRDETNTNQMKRSIPEILGWLSRDNDFPFGVFLMTGSGIAPPDDFSL
                     EPDDIVEIAIDGIGRLVNPVVKDALPSETQTEFGV"
     gene            complement(2876..>3466)
                     /locus_tag="P5179_02555"
     CDS             complement(2876..>3466)
                     /locus_tag="P5179_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94131.1"
                     /translation="LRRLVRWSFSGTVGSVASPAYTRDHIVVARLADRSPEGPQPLRE
                     VRDAIALKVRVEKRVDIATQWLTPIAKKIAAGQPMEQAVQGTEFTVVEIGPIRRGDFV
                     PEVSAGASDGFTGAAFALTTTGQTTGALRIPERGSYIVRLLEKSLDWSTFDQQREQLR
                     QRLARQRRQSTFRDWETYARKSADVKDYRDRFYNFD"
     assembly_gap    3467..3566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3567..4615)
                     /locus_tag="P5179_02560"
     CDS             complement(<3567..4615)
                     /locus_tag="P5179_02560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS94132.1"
                     /translation="MLRFMRNQRFVKALMWIVILSFVGWLGFELGYGGGGRSQATPEV
                     GEIAGEPVYWTEFRREIGTLREYYRRQGNTQIDDFDLDEQVWQQTVQRIILRNAVERA
                     QISVTADELAEELVNNPPSGFERVPEFQRADGQGFDREKYRQFLASMTQERWFQITGM
                     TYSEYESRVLFDLKVQKLQQRIDDNGWITDASLRQAFAEENETIRARVIAAPISLVPD
                     SLIKVSDDEIRSYYQTHLNEFKQRTRARFSYVVIPRRPSPRDVARAAEEIRAAYRQLQ
                     EGADFAETAQRLSEDEGSARDGGDLGTFGRGRMVPAFDSVAFSLPVGEFSQPFHTQFG
                     WHIVKVERRVEDTAW"
     gene            complement(4724..5944)
                     /gene="ilvA"
                     /locus_tag="P5179_02565"
     CDS             complement(4724..5944)
                     /gene="ilvA"
                     /locus_tag="P5179_02565"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRS94133.1"
                     /translation="MAKIPTIDDIHAARERISKYAYVTPLEHSTDFSRLLGNDISHKL
                     ENLQLTGSFKVRGALNAVLSLPKGELAAGVIAASAGNHAQGVAFGAHQLGIKATIVMP
                     LGTPLVKLMAVKRWGAEVVQTGETYDEAADCAASMAHDTGACLIHPFDDPKVIAGQGT
                     IALEILEQKPDVDAVVVPVGGGGLISGIGIALKELHPRVQVIGVQASGADAMAQSLGA
                     GEIRKSPRTLTFADGIRVARPGDLTFAISRKVVDQVVTVDDDEIANAVLLMIETDKSV
                     VEGAGAAPLAALLHKRVGLRGKHVVLIVSGGNIDVNILSRIIERGLVKTGRLIRISVT
                     LGDHPGALAGITDHLAKFGANVLHVHHNRAFARGRLDETTVEFALETRGHEHAREIVQ
                     GLSRAGYLCDVLAG"
     gene            6109..6627
                     /gene="pyrE"
                     /locus_tag="P5179_02570"
     CDS             6109..6627
                     /gene="pyrE"
                     /locus_tag="P5179_02570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRS94134.1"
                     /translation="MELSTLAKAIKEAAYLEGDFVLSSGKRSRYYLDKYLFSTEPRLL
                     EAIAEALSALLPQDFDRLAGVELGAVPLATAVALRTGKPFVIVRKNAKDYGTGKLFEG
                     RLAKGDRVVLVEDVLTTASQAIQAARRLNEFGAEVVKIIYVIDREEGAADNLAAAGFS
                     AEPVMKKSDLGL"
     gene            complement(6666..8891)
                     /locus_tag="P5179_02575"
     CDS             complement(6666..8891)
                     /locus_tag="P5179_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94135.1"
                     /translation="MKSWLDEVDRWVREIVHASTHGRAYDLLVGDPLPEEWGGQAAIA
                     SYLAGCLALHGKEKNDATQVERSREFMLLPDPVHAFAASPAWEAIVALRTAHMLSDTN
                     EEQIRAAMTKTAQNGVRNYLVIRMGIRRFNHAITTANMCDVISRLWPDHPDSAELRQA
                     AEDVWGDWWLIGDNIEIAANYEAFAQVDLLLWAERRGEKARMLDDLMTHFWMDRCLEH
                     MLPVGIVPGYGDTCTMELWADWFALYSLMAAWCGGLRGSRAAWNAERMFSWATTRGWL
                     DQNLSIIDEVPDDPYRAKQGWTQMPRTGWYLALGREWLRKTCAEPTAPPLRPAVTHRT
                     VFAQHVMSDLSWSLTPPAGGPRVADKIVLKTGVEADSPSLMLGCARQVWHDHLDAGAV
                     IAFVSDGTVHLDDTGYMQKYPVFHNLFWADAREKTWLHYGKEEYSRHRELHRPSDYRV
                     SALTGKEVAQLAVIDCEAPHCMPIYHRRETILTRGGVTIIADHVEPYTTGLVGSPVWH
                     VQYVHESTDDWAEVSICEFRGMNGQHCDNAAGRMRILTPTHRDPWTIIQQENNDPYAS
                     PGYREPVTRYFDYWKRSFVLRTALSKPRTLTTDSPNRFYTVLVPLPPSHGQPGGNLVE
                     LLGEEMDLGFVARVRGGMVSLNCTEGQLAGKWGSTDAMHMWWDGKGVFAHRVKAINLP
                     ELRLDTGGQWADLDLFWDNEGISGVISTERATTVTVEVPGTRASFRVHGITEVGRRTD
                     R"
     gene            complement(8905..10092)
                     /locus_tag="P5179_02580"
     CDS             complement(8905..10092)
                     /locus_tag="P5179_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRS94136.1"
                     /translation="MSLHQRLCDAVNEARIGQTALELVSIPSPTGFTREATRYFETAY
                     RELGCEITRIDRIKGSPSGTDAPSVAARLRGTGAAPTLQIDGHIDTIPAAHAPASLVD
                     NVLSGRGASDMKGGLAAVMEAARVLREVRVQLPGDLLLTTHGLHEAPTGHGEGLLALI
                     SEGLHGAAAVVAEGPVDELAITGRGMAIFTITVKTTGKSGHENETPPNTPHPLVAAAE
                     VLRVLEAERVRLQRTMRPYVGSETIFFGQLHGGDFYNRFPVEATIQGTRRYFDDHDFQ
                     DVRKEMEAILARVAEDTGTTITLDFDRIRDGFRVSENERIVHAFRWSYRTLNGKEPPL
                     GAFKSVADVSLLVQAAGIPAVFCGNRGTGAHADREEVDVRELVHQTRMLLGIIVAYFG
                     LET"
     gene            10219..11175
                     /locus_tag="P5179_02585"
     CDS             10219..11175
                     /locus_tag="P5179_02585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRS94137.1"
                     /translation="MSESSLMRAARVAVGQCLGVQKSEQVIVIADAPCLSIGRALFEA
                     SVETGADTQLIEILPRAENGSEPPPPVAAAMAAAHVVFAPTSKSLTHTEARRAASEAG
                     ARIATLPGITEDIMSRALLADYEAIAERTQRVAIALQGKKTIRVTTAAGTDISFSIEG
                     RKVSADTGILRNPGVTNLPAGEAYLAPVEGTGRGLIVIDGCIGSSGVLREPIEVVVEA
                     GMATIIRGNVYAETLVARMDAAGPEARNLAELGIGTNDAARLTGQVLEDEKILGTVHL
                     AFGDNRSMGGIVRAPFHQDGILLRPTVWADGCLILDDGVLMV"
     gene            11195..12019
                     /locus_tag="P5179_02590"
     CDS             11195..12019
                     /locus_tag="P5179_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRS94138.1"
                     /translation="MTENRVMEAFLVVWKYPLFTLGGGTLTLAVLTKTILGLVFVWVV
                     ARVIRWKVTAGTFAKGRIESSTRYTISRILYYTVLFFGVVIVLDTAGLHLSSLTVFSG
                     LMGAGIGFGMQNIASNFISGIILLVERPVRSGDVISIGDTRGVVDSISIRMTRVVSFD
                     NTILLIPNSRLVNEMLVSWTTPSYQFRSSLNVVVPNKHNPARVKELLLEVAGRDSRIL
                     PDPAPAVRFKDMSATTQTYELVVWTRQSSSASEVPSDLRFAIVAHFEENGVSLVTG"
     gene            12088..14442
                     /locus_tag="P5179_02595"
     CDS             12088..14442
                     /locus_tag="P5179_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94139.1"
                     /translation="MPAYTTKNLPLTYLINYQDHLGDIEASVAPLRDAPPNVFHACND
                     MPYLSRAGASSYHDRKGPLAWSFPIGIEEVRERTKATVKWVNAVHAAGVDVVIPYICN
                     QTIGGDPEKRLGFWWFFDRWDDYAEDVGPKPGIDPIEWMQREEDGSLHFNYPYRFTRT
                     DPPLRFAPCPNNPFWHSWLKTMVRLIASYGYDGTFVDNNIMHCRCKYCQQEFAVFLSE
                     TYTKKELKDRYGSTDPSRPKLATAGDVIYWACRQPEYVRQFRDKEPELFKEIFGTTDV
                     RKAKMSEGGTGLHWMKSYKFWLATLERTLGEEGAEHARRVGDVSMLGVRNPRMLCRWA
                     DTQKFWAWTIARRNAELREAAHEVTPRFLIVPNWGNMTGFCHALSRCRDAKNVRLWAR
                     GTDLIFFEDEFFPGTLAPGYTLDLMISYKYAAACGIRSCVLPYQGAESRNLTELATAE
                     AVAFSGDGAFVQMPYHFSEIRQAYRAFFEGNPHLFRGRSSYANVGLLLSFDEVHMENT
                     HHLRESAACARYLAENHVLFDFLCEGQVTLSELARFEVVILPHVEYLPLSGRRAVAEY
                     VARGGRVLVTGNTGEFDEHGRPVKMTDLIGRLRSLVWRRGADGTESVDYASTGRIRWL
                     NDLRKWIPGRIWDVHDIEDAMRDEYAEKVLPALRAKAADTPEHDPRLLGMLNDLAGRD
                     LSTTGRGTPPTLRANAWAGTRADKSIVLHLVNYNIPGPGLPPGEVVPCTEVEVRLRVD
                     RHRVKSVCAANPWESPKELPFHQSGNKVRFTVPRIQIYETIHIQ"
     gene            14569..14928
                     /locus_tag="P5179_02600"
     CDS             14569..14928
                     /locus_tag="P5179_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94140.1"
                     /translation="MRRSLWIMLAAGAILGSGVFGAINAVQSTRLRIVNGLGNWTIRY
                     VYISPTSSDSWGPDRLGSSEVLRPGESQTWTLDPGSYDLRAKDEDEDTYTRRRVCIPE
                     RMTTEWTVELSDRDTNR"
     gene            complement(14989..>15705)
                     /locus_tag="P5179_02605"
     CDS             complement(14989..>15705)
                     /locus_tag="P5179_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94141.1"
                     /translation="GRSLTVDVVRMNADAKIVYSTVAEELGIAHCRAEIVSTPKGDKA
                     AVSVVPSGVRLSDEAALSDEYFREQWPFLVDKVAEQEALADFLGTETGPDSYTITTEP
                     VSVVADNPLEMFHYLPVSNRANEPVFKFLFAKALPDAPAARAKTARDFVVRYETAYGA
                     AAARIKEHWNGIEAALATRKDAVESCSGVELKEALGILKDTFRWNPVSRLTRMYETCI
                     PVQWSAIGKDVWVWRRQKQA"
BASE COUNT         3214 a         4539 c         4504 g         3348 t
ORIGIN      
        1 gttcaacgcc cagagttcgc ggccgtcagg agtgattccg aggccttcaa ctccctcctg
       61 gcagggcacc agagccgcca gcgctccatc agtgcgcctc aatacagaca cgtccttcgt
      121 cacgatattt gcggtgtaaa ggaaatcacc ctcgggtgaa acaagaacca tgtgggagat
      181 tttcccgcac gtgaggattg cgcgcgtgac ttcattcgtt cgcgtgtcaa gctcaaccac
      241 gtaccccttc tgcccggccg tgaaatacgc ccaccgacca tccggcgcca ttgcggcgcc
      301 atgaatgcgt gaatactccc cggtggaaat ctggcgcaca tgcttcgccg cgaccaggtc
      361 gatgagcgag atggtattgc ccgggggtgc gtaattcgag agatatgccc agcggttgtc
      421 cggcgtcacg actatctcgt gcgggtttgg gcctgtcggg atagtttcaa gcacttcata
      481 cgaggtcaaa tcgacaaacg acatggtgtc gtcgtgcttg ttgcatacca gcaggatcgg
      541 ttgttctggc atgtgcgagg tcttctttct cgaggacgga gacgggtgcg ccgaaatcag
      601 gtgttcgatc catcaagcat cttttcgcgg cgaccaattt cgttcaataa ccagaacatc
      661 ggctctgcgc cgccttcacg cgtacagaag aagttgaaca gcataatacc atctgcgcca
      721 tccttccaga gctgacgtgc tatctcccgg taccggtctg gttcgcgctc aaactcaatg
      781 gaaccgtaga tcggcaggtc ggacggcaag agcctgcgga aatccccgat gggaagggga
      841 tgcgtattgc ggaggtaatg ggaaatcgtc acgaaatcga tcagcccttc tcgagcccag
      901 gagacggggt cgagtcccaa cccgagattc tgttccggtg tttccattat tcgtgcgctg
      961 agcagaatgg gcctgcctcg ccggcctgcg accctgttcg tcatggaacg gattgacctc
     1021 acgaattcgg tcatgacggg caggttggat gcctcctgtc cgaaccggaa atagatgggg
     1081 aaacgctgaa agtcgagatc gagaccatcc agagggtagc gttcacacac ctcggtcaac
     1141 tgcgagagca cgtaatcccg gacctctgga acggcgaaat ccagaccagc cggcaaccag
     1201 cttgccacta tcgggtgaac cgggcccaga atagtcttgt ggagatcagg tagagggtct
     1261 cccaccttcc cgaccctcca ctccgggtgt tccagccaga actttgacag gagtacgctg
     1321 tccggctcct catcaacaca gtgtacctcg ttcaatcgga acgtgacgaa ggtttcgcac
     1381 cccttttcct gcgcacgcga aatgaccatt ccaagagggt cgtggccctg ttgaatcaat
     1441 tgccggaaat tgagaatccc gcgttggaac gatgcaggct tgttgacggc gggatccttg
     1501 agcgcttcca gctgttctgc ggtcagccgg tctcctatca attgcacggt ctcgccgcga
     1561 aaattcatat ccatgccgta gttgcagcac atgaaaaacg tcgtgatgtc tgtatccacg
     1621 atctcgtcca cgtaacggtt gacgttgtcg accgtcatgg ggtagtcgta gatgaacatg
     1681 ttgtcagcgt cgcagttgta gattacacgc gttcttcttt cctgcatctg agagcccctt
     1741 cagactccaa attcagtctg cgtttctgat ggtagcgcgt ccttcaccac agggttgacg
     1801 agccgcccga ttccatcaat tgcgatttca acaatgtcgt ccggttcgag agagaaatca
     1861 tccgggggag cgatgccgga acccgtcatg agaaatactc cgaaagggaa gtcattatcg
     1921 cggctcaacc agcccagtat ttcggggata ctccgcttca tctggtttgt gttcgtctcg
     1981 tcgcgaaaga cagccatccc ttccctgata actaccagcg aaatggggag atcacgcgcg
     2041 aaatccgggt cgtccgacac cagaatggtc ggcccgagcg cgcagcaacc gctgtatgtt
     2101 ttcgattggg ccatgtacag ggggttttcg ccctcgatat cacgggacgt gacgtcgttt
     2161 gcgcaagtga agccgatgat ccgctgagcc gggctcaaaa gcaccgtaag ttcgggctct
     2221 ggaatagtcc attcggaatc cgaccgtacg cgaatgcttc cgaacgggcc tgccaccctg
     2281 tgaggtgttg ttttgaagaa cagttccggc cgggctgcgt catacacttc gtcgtaaaaa
     2341 agtttccctc cgtgggactc atcgacgtgt gactggcgca ttctctggta cgtaacacca
     2401 gcgccccaga tctcctgttt gtcgatcggc ggcaaaagat gcctcgaacc cggtgcgggg
     2461 gcgtgttcga gttcccggat gtgcaggcgg tttttgcttc gttctgccag ttccgtgatg
     2521 ccgcgcatgt actcgatcgg atccggcagc gcgagccatg ttgagatgtc tgcgcaaccc
     2581 cgggggtcga tcgccgttgc atcaaccacg gtttcctcga aaacgatccc gagccgggca
     2641 cccgttttcg gttcccagaa cctgcacacc ctcatcagcc gctccttcga tgtcccgggg
     2701 ccaacaatct gtgcgggaca cgttcacagt ctggctttgt tccgtccgag gcgcgtacac
     2761 gagctacaat ataggcagcg ctgcgtgatg ttgtaaacgt tccacggagc agaaaaaaga
     2821 aacgtgcccg aacctcgaga ggtccagaca cgtctttcca ccgtcgtcaa tgccgtcaat
     2881 cgaagttgta gaaccggtcg cggtagtcct ttacgtccgc actcttccgc gcgtacgtct
     2941 cccagtcccg gaacgttgat tggcgtcgtt gacgcgcaag tctctgacga agctgctccc
     3001 gctgctggtc aaacgtgctc caatcaagcg atttctcgag aagccgaacg atgtaggaac
     3061 ccctttccgg gattctgagc gcgcccgtcg tctgacctgt tgtggtgaga gcgaacgccg
     3121 cgccagtgaa accgtctgac gcaccggcgc tgacttcggg gacaaaatcc ccacgccgaa
     3181 tcggtccgat ttccaccacg gtgaactccg taccctggac ggcctgctcc ataggttgtc
     3241 ccgcagcaat tttcttcgct atcggagtca accattgcgt tgcgatgtcg acgcgctttt
     3301 ccacgcgaac tttgagagcg attgcgtctc gaacctctcg caacggttga ggcccttccg
     3361 gagaccgatc ggccagacga gcgaccacaa tgtggtcacg tgtgtaagcc gggctggcaa
     3421 cgctgccaac cgtcccggag aaggaccatc gaacgagacg cctcaannnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnntgcc aggcagtgtc ctcaacacgc cgttcgacct
     3601 tgacaatgtg ccacccgaat tgcgtgtgga acggctggct gaattcgcca acggggagcg
     3661 agaaggcaac cgaatcaaat gcgggtacca tccgtccgcg tccgaacgtc ccgaggtctc
     3721 ccccgtctcg agccgaaccc tcgtcttcgg aaaggcgctg cgcagtttcg gcgaaatctg
     3781 ctccctcctg caattgccgg taagccgccc ggatttcctc ggccgctcgc gcgacatctc
     3841 gcgggcttgg acgacgggga atcactacat atgagaaacg tgctctcgtg cgctgtttga
     3901 actcattcag gtgtgtctga tagtacgagc gaatctcgtc gtcagacacc ttgatcaacg
     3961 aatcaggaac aagactgatg ggagcagcga taacgcgcgc cctgattgtt tcgttctcct
     4021 cagcaaatgc ctgccgcaaa ctggcgtccg taatccaacc gttgtcgtca atcctttgct
     4081 gcagcttctg aactttcagg tcaaacagaa cccggctctc gtattcgctg tacgtcatgc
     4141 cggttatctg gaaccaacgc tcctgggtca tcgaagcgag gaattgacga tacttctcgc
     4201 gatcaaagcc ttgcccgtcg gcacgctgga attcgggaac gcgttcgaat ccactcggtg
     4261 gattgttgac caactcctcc gccagttcat cggccgtaac ggagatttgc gcgcgctcaa
     4321 cagcgtttct gagaatgatc cgttgaaccg tctgttgcca gacctgctcg tcgagatcaa
     4381 aatcgtctat ctgcgtgttc ccctgacgac ggtaatactc cctcagggtg ccgatctccc
     4441 tcctgaattc ggtccagtaa accggttccc ccgcaatttc gcccacttcc ggcgttgcct
     4501 gagaccgtcc tcctcctccg tagccaagct caaagccaag ccagccgacg aaggaaagta
     4561 tgacaatcca catgagcgct tttacaaaac gctgattgcg catgaatcgt agcatcgaac
     4621 agtcacccct gaacggcggg acacttacga cctggagaaa agacggcgta caagactact
     4681 taaaatatcg cgcgcggtta gatatatcaa cccgttcgcc gggtcatccg gcgagcacgt
     4741 cgcaaaggta tcccgctcgg gatagcccct gaacgatttc ccgggcgtgt tcgtgaccac
     4801 gggtttcgag tgcgaattcc accgtggttt cgtccagccg cccccgcgcg aatgctcggt
     4861 tgtgatggac atggagcacg tttgctccga acttggcgag atggtcggtt attcctgcga
     4921 gagcaccggg gtggtctccc agcgtgacac tgattcgtat gagacgccct gttttcacga
     4981 ggcctcgctc gatgatccgg gacagtatgt tcacgtcaat gttcccgccg ctgacaatca
     5041 gcacaacgtg tttgcctctc aatcccacgc gtttgtggag aagcgccgcc agcggcgcgg
     5101 cgcccgcgcc ttccactacc gatttatcgg tctcgatcat caaaaggacc gcgttcgcga
     5161 tctcgtcgtc atccacggta acgacctgat cgaccacttt tcgggaaatg gcgaatgtca
     5221 ggtcgcccgg acgggcgacc cggatgccgt ccgcgaacgt gagtgtgcgc ggtgatttcc
     5281 ggatttcacc cgctccgagc gattgcgcca ttgcgtccgc gccggacgct tgaacgccta
     5341 tcacctgaac acgagggtgc aactctttca gggcgatccc gatgccggaa atcagtccgc
     5401 cgcctcccac cggaacgact accgcatcta cgtcgggttt ctgttccagg atttccagcg
     5461 cgattgttcc ctgtccggcg ataactttcg ggtcgtcgaa cggatgaatc agacacgccc
     5521 ccgtatcgtg tgccattgag gcggcacagt cagcagcctc atcgtatgtt tcacccgtct
     5581 ggacgacttc ggcgccccac ctcttcacgg ccatcagttt cacaagcggg gtgcccagcg
     5641 gcatcacgat tgttgctttg atgccgagtt gatgcgctcc gaatgccact ccctgggcat
     5701 ggttccctgc ggaggccgct attacgcctg cggccaattc gcctttgggc agagacagca
     5761 cggcgttgag tgccccacgt actttgaacg agccggtaag ctggaggttt tccagcttgt
     5821 gggagatgtc attcccgaga aggcgcgaaa agtccgtgga gtgttcgagc ggggttacat
     5881 acgcgtactt ggatattcgt tcccgcgctg cgtgaatgtc gtcgatcgtc ggaattttgg
     5941 ccatcgtaat gaatgtcctg ttgcggagag aaagcctggc agggtactgt ttaaattcgt
     6001 tgacaaatgc ccgtagtcgc aagcggctgc ctcgaaaggc cccatcaagg gacggcttgc
     6061 ggatgcgtgt tcgggaattg cgaccagtca caaaccagga gaactaccgt ggagctctct
     6121 acacttgcaa aggcgatcaa ggaagcggca tatctggagg gcgatttcgt tctgagcagc
     6181 gggaaaagga gcagatacta tctcgacaaa tacctgtttt cgacggaacc gagactcctc
     6241 gaggcgatcg ctgaagccct gtcggcgctg ctgccacagg atttcgaccg gctcgcggga
     6301 gtggagctcg gcgcggtccc tctcgctacc gcggtcgcgc tgaggacagg caagcctttt
     6361 gtaatcgtcc gcaagaatgc aaaggattac ggtaccggca agctgttcga ggggaggctt
     6421 gcgaaaggcg acagggttgt tcttgtggag gacgttctga ccacggcgtc acaggcaatt
     6481 caagcggcgc gccgattgaa cgaatttggc gcggaggtgg tgaagatcat ctatgtgatt
     6541 gaccgggagg aaggagcggc agacaacctc gcggcggcgg gtttctcagc agagcccgtg
     6601 atgaaaaaga gcgaccttgg cttgtaagac gagggcaccc cggcgggagt gccccggttc
     6661 gaccttcacc ggtcggttct gcgtcctact tccgtgattc cgtggacgcg gaaagaagcg
     6721 cgtgtacccg gtacctcgac cgtcacggtc gttgcgcgct cggtcgagat cacgccgctt
     6781 attccctcat tgtcccagaa gagatccagg tctgcccact ggccgcccgt gtccagacga
     6841 agttcgggaa ggttgatggc cttcacgcgg tgcgcgaaaa cgcctttgcc gtcccaccac
     6901 atatgcatgg cgtcggtgct cccccatttg cccgccagtt gcccttcggt gcagttgagc
     6961 gataccatgc caccgcgcac acgcgccacg aaaccgagat ccatttcctc gccgagcaat
     7021 tctacgaggt tgccaccagg ctggccatga gatgggggca acgggacaag caccgtgtag
     7081 aaccggttgg gcgaatcagt ggtaagcgtc ctcggcttgg aaagcgcggt gcggagcacg
     7141 aagctgcgct tccagtaatc gaaataccgc gtgaccggtt cccggtaacc gggcgaggcg
     7201 tacgggtcgt tgttttcctg ctgaatgatc gtccaaggat ccctgtgcgt cggcgtcagg
     7261 attctcatgc gtccggctgc gttatcgcaa tgctggccgt tcatgccgcg gaactcacat
     7321 atggacacct cggcccagtc gtcagtgctc tcgtgaacgt actgaacgtg ccagaccggg
     7381 gagccgacca atcccgtggt atacggctcc acatggtcgg cgatgatcgt gacgccgccc
     7441 cttgtgagga ttgtctcgcg ccggtggtag atgggcatgc aatggggggc ctcacagtcg
     7501 ataacggcga gctgggcaac ctcctttccg gtcagcgcgg acacgcggta gtcgctgggc
     7561 cgatgaagct ccctgtgtct tgagtactcc tctttgccgt aatggagcca cgtcttctcg
     7621 cgggcatccg cccagaagag gttgtgaaac accgggtatt tctgcatgta tccggtgtcg
     7681 tcgaggtgaa ccgttccgtc cgatacgaac gcgattacgg cacctgcgtc cagatggtcg
     7741 tgccagacct ggcgggcaca acccagcatg agcgaggggc tgtcagcctc cacacccgtt
     7801 ttgagaacga tcttgtctgc cactcgggga ccaccggcgg gcggtgtcaa agaccaggaa
     7861 aggtcgctca tcacgtgttg cgcaaaaacc gttctatggg tgaccgccgg acgcagaggc
     7921 ggtgcggtgg gttccgcaca cgttttcctc aaccactccc ggccaagcgc cagataccag
     7981 ccggtgcggg gcatctgtgt ccagccttgt ttcgcccggt aagggtcatc tggaacctca
     8041 tcaatgatgc tgagattctg gtccagccat ccccgggtgg ttgcccagga gaacatgcgc
     8101 tctgcgttcc atgcggctct gctcccccgc aaacctccgc accaggctgc catcagggag
     8161 taaagcgcga accagtctgc ccacagctcc attgtgcatg tgtcgccgta tcccgggacg
     8221 atgccgaccg gaagcatatg ctccagacac cggtccatcc aaaaatgcgt catgagatca
     8281 tccagcatcc gcgccttctc acctcgccgc tccgcccaca ggagcaggtc aacctgcgcg
     8341 aaagcctcgt aattggcagc aatctctatg ttgtcgccga tcaaccacca gtctccccac
     8401 acatcctcag cagcttgacg caattctgcg gagtccggat gatccggcca gaggcggctg
     8461 atgacatcac acatattggc agtggtgatg gcatggttga accgtcgaat tcccatccga
     8521 atcacgagat agttgcggac tccattttgc gctgtctttg tcatagctgc gcggatctgc
     8581 tcttcgttgg tgtctgagag catatgtgca gtgcgaaggg caacaatggc ttcccaggcg
     8641 gggctggccg caaatgcgtg taccgggtcg ggaagaagca tgaactcacg agaacgctca
     8701 acctgggttg cgtcattctt ttccttgccg tgcagagcca agcaaccggc cagataggaa
     8761 gcgatcgccg cctgtccgcc ccattcttct ggcaacggat cgcccaccag gaggtcgtat
     8821 gcacgcccat gagtggacgc atgcacgatc tcgcggaccc agcggtcgac ctcgtcaagc
     8881 cacgatttca tagtcctttg cctttcacgt ctcgagtcca aaatacgcca caatgattcc
     8941 gaggagcatc cgggtctgat ggaccagttc ccgaacgtcc acttcctccc tgtctgcgtg
     9001 cgcaccagtg cctctgtttc cacagaacac cgccggaatt cctgcggctt gaaccagcaa
     9061 tgatacgtct gcaaccgact taaacgcccc gagcggtggc tctttgccgt tcaatgtgcg
     9121 gtaactccac cggaatgcat gcacgattcg ctcattctca gacaccctga aaccatcgcg
     9181 gattcgatcg aaatccagcg tgattgtcgt acctgtgtct tcagcaaccc gcgcgaggat
     9241 cgcctccatt tccttcctga cgtcctggaa atcgtggtcg tcaaaatacc gacgtgttcc
     9301 ctgaatcgtt gcttccaccg ggaaacgatt gtaaaaatcc ccgccatgga gttgtccaaa
     9361 gaagatcgtc tccgaaccta cgtacggacg catggtgcgc tgcaaacgca cacgttccgc
     9421 ttccagaacc ctcaggacct cagcggcagc cacgagcgga tgcggcgtat tcggcggcgt
     9481 ttcgttttcg tgcccgcttt tgcctgtcgt tttgaccgta atggtgaaaa tggccatccc
     9541 acggcctgtg attgccagtt catctaccgg tccttccgcc acaacagcgg ccgcgccatg
     9601 cagaccttca ctgataagcg cgagcagccc ttctccgtgg ccggttggtg cctcgtgaag
     9661 cccgtgtgtc gtgagcagga ggtcaccggg taactggact cgcacctcgc ggagaacacg
     9721 cgcggcctcc atcacagcag ccagcccacc cttcatatct gacgcgcctc gtcccgaaag
     9781 cacgttgtca accagcgagg cgggggcgtg tgctgccgga atggtgtcaa tatgtccgtc
     9841 tatctggagt gtgggtgcgg cacccgtgcc gcgcagtctc gctgccacgc tgggcgcatc
     9901 cgttccggag ggagaccctt tgatcctgtc aatgcgcgtg atttcgcagc caagctcgcg
     9961 gtacgctgtt tcgaagtatc gagtcgcttc gcgagtgaaa ccggtggggc tcggtatgga
    10021 gaccagttcc aacgccgtct gcccgattcg agcttcgttg acggcgtcgc acaatcgttg
    10081 gtgcaacgac ataggatcgt ttccccgtag agtggtataa ttcctaacgc tgcgtcgagg
    10141 cttttggagc ctggatacgt aacctaacgc cgcgcgtcca gaccgcgcaa ccacggagtt
    10201 cttcgaaaga ggtcgtccat gagcgaatcg tctcttatgc gggctgctcg agtggcagtc
    10261 gggcaatgcc tgggggtcca gaagtcggaa caggttatcg tcatcgcgga tgcgccctgt
    10321 ctatcgatag gtcgtgcctt gttcgaagca tctgtggaga ccggagcgga cactcagttg
    10381 attgaaattc ttccgcgggc ggaaaacgga tctgaaccgc cgcccccggt ggcggcagca
    10441 atggccgccg cgcacgtggt gtttgcccct acctcgaagt cgctcacgca tacagaagcc
    10501 cggcgcgcgg cttcggaggc gggagcccgc atagcaacgc ttcccggcat cacggaagat
    10561 atcatgtctc gcgcgcttct tgcagattac gaggcaatcg ccgaacggac gcaacgcgtg
    10621 gcgattgcgt tacagggaaa aaagacaatc cgtgtcacga ctgctgcggg cactgatatt
    10681 tcgttctcga tcgaaggtcg gaaggtctcg gccgatacag gcatcctgag aaacccgggg
    10741 gttaccaacc tgccggccgg tgaggcgtat ctcgcaccgg tcgaaggaac cggccgtggc
    10801 ctgatcgtga ttgatggttg catcggcagc agcggcgtgc tgagagaacc aatcgaggtt
    10861 gttgtcgagg cggggatggc aacgattatt cggggcaatg tgtatgcgga aacgctcgtc
    10921 gcacggatgg atgccgcagg accggaagca agaaacctcg ctgagctggg aattggaacg
    10981 aatgatgcgg cgcgattgac cgggcaggtt ctggaggatg agaagatact gggcaccgtg
    11041 cacctggcat ttggagacaa tcgttctatg ggaggtatcg tgcgcgcccc gttccatcag
    11101 gacggtattc ttttgcgacc cactgtatgg gccgatgggt gtctgattct cgatgacggc
    11161 gttctaatgg tgtgaggccg aagggagtcc gaaaatgacg gagaaccggg tgatggaagc
    11221 gttcctggtg gtctggaaat atcctctttt caccctcggg ggaggcacac ttacgctggc
    11281 tgtgctcacg aaaactatcc tcggtcttgt gttcgtctgg gttgtcgctc gagtgatccg
    11341 gtggaaagtg acggcgggaa cgtttgcgaa agggaggata gagtcctcca cgcgatatac
    11401 gatctctcga atcctgtact acactgttct tttctttgga gttgtgatcg ttctggacac
    11461 ggcgggtctg cacctttcct cccttacggt tttcagtggc ctgatgggtg ccggaatcgg
    11521 tttcggcatg cagaacattg cgtccaattt catcagcggc attatcctgc tcgtggagcg
    11581 gcccgtgcgc tcgggagatg tgatatcaat tggcgatacg cgcggagtcg ttgattccat
    11641 aagtatccgc atgacccggg ttgtatcgtt cgacaacacg attcttctca ttccgaacag
    11701 ccgattggtg aatgagatgc tggtcagttg gacaaccccc tcttaccagt tccgttcttc
    11761 actcaacgtc gttgtgccta acaaacacaa tccggcgcgc gtgaaagagt tgctgcttga
    11821 ggtggcgggc agggatagcc gaatacttcc tgacccggca cctgcggtgc gattcaagga
    11881 catgtcggcg acaacccaga cgtacgaact ggtagtgtgg accaggcaat cgagttctgc
    11941 atctgaagtt cccagcgacc tgcgttttgc gattgtcgcg catttcgagg aaaacggggt
    12001 ctctcttgtg acagggtagc tcgaactgca acgtgccgcg gaattccact aaattcgggg
    12061 gtaacctcag aaagaggggt acttacgatg cctgcctaca cgacgaaaaa cctgcctctg
    12121 acctatctga tcaactacca ggatcacctt ggcgacatcg aggcgtcggt tgccccactc
    12181 cgggacgcac ccccgaacgt attccacgca tgcaacgaca tgccctatct ctcccgggcc
    12241 ggcgcctcga gttaccacga cagaaaggga cctctggcgt ggtcattccc aatcgggatt
    12301 gaggaggtcc gcgaacggac gaaagcgact gtcaaatggg tgaatgccgt tcacgctgcc
    12361 ggtgtcgatg ttgtcattcc gtacatctgc aatcagacca taggcggcga ccctgagaaa
    12421 cgcctcggtt tctggtggtt ttttgaccgc tgggatgatt acgccgagga tgttggcccc
    12481 aaaccgggga tcgatccgat agaatggatg caaagggaag aggatgggag ccttcacttc
    12541 aattacccct acaggttcac gcgcaccgac ccgcctttgc gttttgcccc gtgcccgaac
    12601 aatccgttct ggcacagctg gctcaaaacg atggtaaggc tcatcgcatc gtacggctac
    12661 gacggaacgt ttgtcgacaa caacatcatg cactgccgtt gcaagtattg ccagcaggag
    12721 ttcgcggtat tcctttcgga aacgtataca aaaaaagagc ttaaggaccg ttatgggtcg
    12781 accgatccgt ctcgtcccaa acttgccacc gccggagacg ttatctactg ggcgtgccgt
    12841 caacccgagt atgtcaggca gttccgcgac aaggaaccgg agttgttcaa agagatattc
    12901 gggaccacag acgtccgcaa agcaaagatg agtgagggag ggacgggcct ccactggatg
    12961 aaatcataca agttctggtt ggcaacgctt gagcgcaccc ttggtgagga aggtgccgaa
    13021 catgcgcggc gtgtcggtga tgtctccatg ctgggagttc gcaacccccg catgttatgc
    13081 cggtgggctg atacgcagaa attctgggct tggacgatcg cccgacggaa cgcggaactc
    13141 cgagaagccg cgcacgaggt gacaccgcgc tttctcatcg tcccgaactg gggtaacatg
    13201 acaggtttct gccacgcttt gagccgttgc cgggacgcca aaaacgtacg tctgtgggcg
    13261 cgcggcacgg atctgatctt ctttgaggat gaattctttc ccggaacgct ggcccccggg
    13321 tataccctcg acctcatgat ctcctacaag tacgcggcag cgtgcggaat tcgttcgtgc
    13381 gtacttccgt atcagggagc ggagagccgt aacctgactg agcttgcgac ggctgaagcg
    13441 gtggcgtttt ccggagacgg cgcgttcgtt cagatgccgt accacttctc cgaaatccgg
    13501 caggcctacc gggcgttttt cgaggggaat ccgcatttgt tccgtggacg ttcctcgtac
    13561 gcgaacgtgg ggctgctttt gagctttgat gaagtgcata tggagaacac acaccatctg
    13621 cgcgaatcag cggcgtgtgc acgatatctt gccgaaaatc acgtcctgtt cgatttcctg
    13681 tgcgaaggcc aggtgacgct ttcagaactt gcgcgcttcg aagtcgtaat tctgccgcac
    13741 gtggagtacc tgccgctctc tggcaggagg gcggttgcag aatacgtcgc gcggggcggg
    13801 cgggtgctcg tcacgggaaa tacgggtgag ttcgatgaac atggccgtcc tgtgaaaatg
    13861 acggacctga tcggccgcct gaggtcgctt gtatggcgtc gcggtgccga cggtaccgag
    13921 tctgtggact atgcgtccac gggaagaatt cgttggttga acgacctgag gaagtggata
    13981 cccggacgca tctgggacgt tcacgacatc gaagatgcga tgcgcgacga atacgcggaa
    14041 aaggttcttc cggcgctccg tgcgaaggcc gcggacacgc cggagcacga cccgaggtta
    14101 ctgggcatgc tgaacgatct ggcgggacga gatttgtcaa caacggggcg ggggacaccc
    14161 ccaacgctgc gcgcgaacgc gtgggcggga acacgagccg acaagtcaat cgtgttgcac
    14221 ctggtcaatt ataacatccc cggccccggc ctcccaccgg gtgaagttgt cccgtgtacg
    14281 gaggtggaag tgcgattgcg cgttgacagg caccgcgtga agtccgtgtg cgcggcaaat
    14341 ccgtgggagt ccccgaaaga gctgccgttc catcagagtg gcaacaaagt acgattcacc
    14401 gtgcctcgca tacagatata cgagactatt cacattcagt gaacaagtgt atattttgca
    14461 cgaaggcctg tctgagttcc atgcaggttt tgcatcgact gcacaccctg accccggcgc
    14521 tgcagttgtg ttgacgaggg gggccacacc agggaaaggg aggctagcat gcggcgttcg
    14581 ctgtggataa tgctcgctgc aggggcgatt ctcgggtctg gagtgttcgg ggcgatcaac
    14641 gccgtccagt ccacacggct ccgaatcgtg aacgggctcg gaaactggac tatccgatac
    14701 gtgtacatct cgcccacgtc cagcgacagt tggggaccgg accgtttggg aagcagcgag
    14761 gtgctgcgtc cgggcgaatc acaaacctgg acactggacc cggggagcta tgatctgcgg
    14821 gcaaaagacg aggacgaaga cacgtacaca cgcagacggg tgtgcatccc ggaacgcatg
    14881 acaaccgaat ggaccgtgga actttccgac agagacacca atcggtaaca cctgatacga
    14941 aataatgcga gggcatcctt ttcgagatgc cctcgccggg taccgtactc aggcttgttt
    15001 ctgtcgcctc cacacccaga cgtctttccc gatagcactc cactgcacgg ggatgcacgt
    15061 ctcgtacatc ctcgtgagcc gcgaaaccgg gttccatctg aacgtgtctt tcaaaatccc
    15121 cagcgcctct ttcagctcca cgccggagca actttccacg gcatcctttc tcgttgcgag
    15181 tgcagcttct atgccgttcc agtgctcttt gatacgagcg gctgcagcgc cgtacgcggt
    15241 ctcgtaccgc acgacgaagt cgcgcgcggt tttcgccctt gccgccggcg catcaggcaa
    15301 cgccttcgca aacaggaact tgaacaccgg ctcattcgct ctgttggaaa ctggcaggta
    15361 gtggaacatt tcgagggggt tgtctgcgac gactgaaacc ggttcagtcg tgattgtgta
    15421 ggaatcggga ccggtttccg tacccaggaa atcggcaagt gcctcctgtt cggcaacctt
    15481 atccaccagg aagggccact gctcgcggaa atactcgtcg ctcagtgcgg cctcatccga
    15541 aagcctcacg ccgctcggta ccactgaaac cgcagctttg tcacctttgg gcgtggaaac
    15601 gatctcggcc cggcagtgag cgattccgag ttcctccgca actgtggaat acacaatctt
    15661 tgcgtccgca ttcatgcgca cgacatcaac ggtgagactc ctccc
//
LOCUS       DAOTNG010000030        15669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3736_length_15669_cov_5.955489, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000030 DAOTNG010000000
VERSION     DAOTNG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_15669_cov_5.955489"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="P5179_02610"
     CDS             complement(<1..166)
                     /locus_tag="P5179_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRS94142.1"
                     /translation="MSAYPDIPEEHLDALAIGPHRDDVELSCGATLVKLRKLGYRVGI
                     ADLTAGEMGTR"
     gene            complement(163..630)
                     /locus_tag="P5179_02615"
     CDS             complement(163..630)
                     /locus_tag="P5179_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS94143.1"
                     /translation="MNPQDSFPPANRRCVIALVDIAGFGDYVRTHASGDVLRLVDAFC
                     RHVECTVRRAGGEVVKMCGDAALVVFAETPVNQSVNALRMLRSRDGELHLATGESVRV
                     TIRAHYASVACGFIGTRGMRDVAGAGVNELFCLPAGEFVLSDALQHALNEDTP"
     gene            812..1543
                     /locus_tag="P5179_02620"
     CDS             812..1543
                     /locus_tag="P5179_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRS94144.1"
                     /translation="MSGATPQALDKAFQVVDAVTRLKGVTGALLIDPDGSTIAQDFRS
                     DDDLKRASAIGTTLPFELTKAAKSLKMGLVSDLTIQTVHGALRVCQHDGIWLLVFIGP
                     RANLGMLNIEMRAVRELFASVFQDDVKRGMDAEKQRILEVIESTGAVDSVIDKRRGDL
                     KAMRMLHEMVFRIAIDAGIGRETISRTINDINYRIYRDSLVDIGFDFFNRKALDTYDP
                     ALAERVMEEQIKGIAGLVTAQKGKG"
     gene            complement(1563..2897)
                     /locus_tag="P5179_02625"
     CDS             complement(1563..2897)
                     /locus_tag="P5179_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRS94145.1"
                     /translation="MASKKGSGTAIRTGVFVILGFALFVGALFVLGARKQLFEDVYEI
                     YSTYEDVGGLQEGAFVRIAGINVGTVKKIALPELSQNRVVVTFSIRGDARRLIRRDSK
                     AIIDTEGLLGARIVIIVPGSPDEPEVPEGGTIQGQSPIQLHRFSANIDRALEHLDAVV
                     INSADLLAAGSSILQKVDAGQGTLGELVNNPALYDTVISSLAQFRRVAATADGTLRNV
                     QGLAGEIAGSVDRTLTHYSVAADTLRDAGSEFRRTAKATNTILRDLQEGRGTLGKLMT
                     DDSAYVALNNTIASSKQMLDRGTEAIAEITRAANSIARSAEQTRATIDLVSRNVMQGQ
                     GTVGRLLTDDSVYTRLDRVLANLDISSQKLAVNMEALRTNWLFRGYFEDAGYWENTKR
                     TMELVEQRNIRLNEWERRLQRIQADLEARERRLEAQSRQAAPARRDTSQESR"
     gene            complement(2904..3659)
                     /locus_tag="P5179_02630"
     CDS             complement(2904..3659)
                     /locus_tag="P5179_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS94146.1"
                     /translation="MIDLVNLHKSFGDLHVLRGVTFSVRKGETHVVLGRSGTGKSVSL
                     KCIVGLLDPDEGAVLAEGVEVAQMDQTTLEAWRSRVGFLFQGGALYDSMSVLENMVFP
                     LRSRKDLSEEDKTDLAHEKLRWVGLEGAVDKNPSDLSGGMRKRCALARTLMTEPEVIL
                     YDEPTTGLDPVTSNEISQLIARLKDERGVTGIVVTHDMPCARLVADSVTFLHRGSVLV
                     SGTWDTIRSCDKPLVQFFMASEFTREPLDIGEE"
     gene            3818..4459
                     /locus_tag="P5179_02635"
     CDS             3818..4459
                     /locus_tag="P5179_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS94147.1"
                     /translation="MAKRTTIKITPRVPRAPFSAPPSRVHDRTRRQQKVGEPFEDGTP
                     EFQKPEYRFCPRCGTLLEPALEHDVVRPTCPECGFIVYYNPAPAVGAVITREGEILLV
                     KRAHPPKEGLWSLPAGFMEFGEKQLDALAREVREETGIEIRAATLLSVEDAADDPRTH
                     ALLIAFIADEWEGEPRPGDDASEVRWWPVDDLPQMAWQNHVRVVEKAREWLGL"
     gene            4444..4905
                     /gene="ruvX"
                     /locus_tag="P5179_02640"
     CDS             4444..4905
                     /gene="ruvX"
                     /locus_tag="P5179_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRS94148.1"
                     /translation="MARPVRVLAVDYGDRRVGLAVSDELGIAAHGLPTLQVKSRKEAV
                     RAVVQAAKEHGVSRIVIGLPLNMDGTEGPRALVTREFGRNCEDALAAERCEIRVEMLD
                     ERLTTMRARAALREQGLRERSQRAKVDRVSATVLLQDYLAAQSATEGNDDG"
     gene            4895..5146
                     /locus_tag="P5179_02645"
     CDS             4895..5146
                     /locus_tag="P5179_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94149.1"
                     /translation="MTDDTRKGADCLSWPTIIHVAATDVGWLVILLPLSVVFRALLLI
                     ATGYYRLRRRSRFLGGAYALGVVGALAWLFARILRLWRD"
     gene            5146..6270
                     /gene="mltG"
                     /locus_tag="P5179_02650"
     CDS             5146..6270
                     /gene="mltG"
                     /locus_tag="P5179_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRS94150.1"
                     /translation="MRNDPGNPSPQVSEGRERDSGCTDSVTVKALAGLAGVLALFAVV
                     VSAGWVALDTVRFLYTPPRGAGLYRVEIPAGATPREIGRILESHGIVRSARIFAYASW
                     RRGLTRAPRGGEYWLDGGQNVFRVLDQIARSGRPIRRVTIPEGYTVAEIAGVVERTLG
                     IPRDSMLALSRDTALVRRYALKGAPSLEGFLFPDTYFIDSGVTAEHVVRVLTQQFHRV
                     FTPEMVRRAEQLGMTPLEVITLASVIEKEAAVASERAVIAQVFYKRLKTGMGLESDPT
                     VKYAMGSGPVNLSLRDIQLDSPYNTYRYRGLPPGPICNPGKAALDAALYPADTDFLYF
                     VANWDGTHAFSRTLGEHNAAKAVSKRRYREWKAARGAAGT"
     gene            complement(6228..7613)
                     /locus_tag="P5179_02655"
     CDS             complement(6228..7613)
                     /locus_tag="P5179_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS94151.1"
                     /translation="MNEVLNWIQAVFAEYNLLFAKLLVHVAIVAMVASLLLRFRKFVA
                     LLLHEHPGTRERTEFGLVLGVIAAAGAWTRINLGYTSADLLVVAPLLSGFIMGTAAGV
                     WTGIIGGLVPLRHGELLAPIAGSIVGISAGITRASLKDPGSVWFFSPVPFSDTVATWR
                     RWRTERVLDPRLVVLLVGAGAEVFRTECARWTNPRWLFSYSPTEPVAYYIIVLMCLTS
                     VGVSLKIWNTPRMEARLRRQEALLAEARLDALRDQINPHFLFNTLSTINSLIRTHPDQ
                     ARIVIIKLSAILRRLLYSRENLCPLKAELDFVEDYLNIERIRFGADRLRLQKEIAPDV
                     VNARVPMMLLQPIVENAVKHGIGGREQGGTVTIRAHAEAGTLAIEVEDDGVGMTGRQL
                     EQSIRKGIGLSNIRERITSLYGESGHLSLQSELGKGTLVKILLPLDTSTGFPEHPRQV
                     PAAPRAAFHSR"
     gene            complement(7610..8263)
                     /locus_tag="P5179_02660"
     CDS             complement(7610..8263)
                     /locus_tag="P5179_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRS94152.1"
                     /translation="MIEHTTVAPVYLLCDMDGTLVDTEGLKDEALRRAIVDARGTDLD
                     PEEQTRIYSRYVGMPGIDIARAMIRHYCLAISADELWELREQHRRVLYSDAKALQARV
                     LSPVVALVERFREGIAVTGRGKTVLVTTAGGDQVHAVLEATKLGHLFDLVVSGREKSR
                     DNPACYRAALDACGALPEECLALEDSVVGYEAARSLDIPCLLLPNSYTRHQRLEKRP"
     gene            complement(8285..9388)
                     /locus_tag="P5179_02665"
     CDS             complement(8285..9388)
                     /locus_tag="P5179_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94153.1"
                     /translation="METESPSRIELPDPFVCDDGTRLTDPDQWPQRRREMLRTIVDTE
                     YGGLPPTPAGVRGERLSTNKMARILDAQYSQYRILLEDHPAFHIRLDLVVPPGEGPFP
                     VILNGDGCWRTLTDEILLAIMRRGFISAQFSRVEVVPDIALPERNSGLYAIYPDARFG
                     ALAAWAWGYHRCIDFLTTLDIVDTHAIAVTGHSRGGKAALLAGATDERVALTAPNGSG
                     CGGAGCYRWQGPKSETMAIIRGPFEFWFGPDLWQWIGREQEMPFDQHFLKAACAPRAM
                     LSTEGLGDLWANPEGTWQTYLAAREVFRFLGVEHNIGIHFREGGHQHGIADWTALLDF
                     AQWKFRGYPLPYRFDTSPFTHLPKAFSWSCPAT"
     gene            complement(9552..10190)
                     /locus_tag="P5179_02670"
     CDS             complement(9552..10190)
                     /locus_tag="P5179_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRS94154.1"
                     /translation="MKKNLWIFGSLAALSLAAVAVFAQTPSSPAADDEDGTPAMGMFA
                     MRGMGAGMGPGMCPPGTGMGMAAPGGQAMMCGRRGMGAGMAGRGMGLMRLQALNLTSD
                     QWARVRRIQEDFQKQIIPKQSEIRVAQIDLQQLLQATQPNMNQVNAKLTTIGNLRTQI
                     QTLRTQEMIQIRGVLTQEQLTKFLDPSWQPPCAMQGMQAPAPGAGRRPMMNR"
     gene            10461..11393
                     /gene="trxB"
                     /locus_tag="P5179_02675"
     CDS             10461..11393
                     /gene="trxB"
                     /locus_tag="P5179_02675"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRS94155.1"
                     /translation="MENHAKVLILGSGPAGLTAAIYAARADLAPLVVEGGQPGGQLII
                     TTEVENFPGFPEGIMGPELMDNMHRQAERFGARFVYGVATEVDLSRRPFVVDVEGTNY
                     TADTLIISTGASARLLGLESEARLMGHGVSACATCDGAFFRNQKVLVVGGGDSALEEA
                     NFLTKFASEVCIVHRRDELRGSQIMQKRVLANPKIRMIWNSVVEDILGDPATGVTAAV
                     LRNVKTNALTEEPCQGVFVAIGHSPNTEVFKGKLDLDEKGYIITKPHSTKTNVTGVFA
                     CGDVMDSSYRQAITAAGTGCMAALDAERFLAENE"
     gene            11672..>15669
                     /locus_tag="P5179_02680"
     CDS             11672..>15669
                     /locus_tag="P5179_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRS94156.1"
                     /translation="MRARIPGILSIALLGTVGQAAAAPAPAPPPVARPARAASDHVPL
                     AMRWLDINNFRCPITNYAFIGKNEIDNRAGGEWPAGSGEEYLFGSGILIAGTVNQEPF
                     TETLGARTAYSFRPSSTNPLGWQDLGATESGAGSRAILTDPRAAIGYNPSGRVSEMSP
                     LTKVFVSAEPDWPLGVPTSVLDTYTEYDDQDPTRWNTGEGSNSGADTGEWSGTPDNTT
                     VATYGLGVKVKQTTYSWNYAGNSDIHFIVFDVENIRADGRAINNCFVGMVADPDIGSN
                     ASDDMTGFDATRNLGYAYDSDFSEVGFRRVPGFLGYRFLKSPVADHDIDRNGDGVIDG
                     NSVMINGVPVKDVHAGEQIGLHAFKSFNQASGDPDTEWEWYMAMAGHNFREDQAQIYQ
                     PFDFSNTPNDQRFLQSTGPFTLQPAQPVRIVVAMMVAKAAGNQGDPIEVRVAELLRTS
                     DVAQSIFDNNFVLPRPPVAPRLTARAGDGRIYLSWDTAAETTPDPFYAVASNPASTLY
                     DPRYRQYDFEGYRVWRSSTGNPNDWQLLADYDVVSAIPRDAVISLQAGTYSGTLIYDA
                     ATTEGYFLSRGGTDLFNGHEYLVMVEGGTQRVKVYDVSLGGQELPCNTSADGADWWFY
                     VGDNSRFELFDGNLNPLSSDPERNANGTYDQYVPGMVMYLGGMALRFADVPAPAGTAI
                     WRVVSSKEENFGGNTSVAHSYIDGGLRNGQLYHYAVTAYDFQLSSPRSLESAKSLSMT
                     SISPRHDPSDTTRVTTAVHTNGVADCAVNVQVVDPMALTGHTYQIGFNPDGTWFVRDT
                     NLPAGQDIVINNQTNLSGDLNYPIVDGVLVNVIAPPVGIKSTSYSPSENKWFVERSAG
                     YEALTGQEWGWGTTVTAKDYARVEIRFNGGSTLAPVYLRGGTPNYSYVGAGTIPAEIW
                     DVTNNRRLAAAFVEQNGAVSRDGNWMPATAADGGREYLFVLAQPYNPDTLSADWDFFR
                     SKSILADASQFPLMTGSWIIAKEGMAPATGDVWAISPYLGNTPEDVFEYTTTHAPVAS
                     SLELPILAAGRREVVTFPVRGTLWNARSAQMMFLVGANTVQSVSIVHHAFQGQQGAQA
                     VATLAGDTVRVAMSGEQEVSLSNQALVVLSMTIRADADTGITHLVWLPAPLTSVDEEG
                     VNLVNGELYVSFIRYGDVTADGSLTAQDATWILQSVVGIRTGVDVETADVSDNGSITA
                     YDAALVLFKVIHPEYVFPVRGGPLIKPSARAPVSLVWERTDAGWSLVVEDASGILAGD
                     VALALPDEVQVQVAADGAFAYRQDHRTLYVSFVHSAGDDRILFTVLTSAMETPALTAA
                     SLNEGAILLKFA"
BASE COUNT         2988 a         4612 c         5019 g         3050 t
ORIGIN      
        1 cgcgggtgcc catctctccg gcggtcaggt cggctatgcc gacccggtat ccgagcttcc
       61 tcagtttcac cagcgtggcg ccgcagctca gttccacgtc atcgcggtgg ggtccgattg
      121 cgagggcatc gaggtgctct tccggaatgt cggggtacgc gctcacggtg tgtcctcatt
      181 caaagcgtgt tgaagggcgt cagacaggac gaactccccg gcgggcagac agaacagctc
      241 attcacgccg gcaccggcga catcgcgcat tccgcgggtg ccgataaatc cgcacgccac
      301 gctcgcgtaa tgcgcgcgga tggtgacgcg cacggattcc ccggtggcca gatggagttc
      361 cccatcgcga gatcgaagca tgcgcaacgc gttgacggac tggttcaccg gggtctcggc
      421 aaatacgaca agggcagcgt cgccacacat tttgacgacc tcgccgcccg cccggcgaac
      481 ggtgcattcg acgtgccggc agaaggcatc aaccaggcgg agaacgtcgc cgctggcgtg
      541 tgttcgaacg taatcgccga agcccgcgat gtcaacgagc gcgatgacac aacgccggtt
      601 ggcgggggga aacgaatcct gcgggttcat gccgggctcc tgctcccatt cccgggtcac
      661 ggatcggcaa aaggcggccc gggcaacacg ggcgcgtgca ctctgttgac gcgacagtcg
      721 tgtggaacga tactcataag tattggacgg atcgcgtcgg gcgcaatccg tccggattgt
      781 gcgcgggaca actcctgcaa cgaggttttc catgagcggg gcgactcctc aggcgctgga
      841 caaggccttt caggttgttg acgccgtcac gcggttgaag ggcgttacgg gggcgttgct
      901 tatcgatccc gacgggtcaa ccattgcgca ggatttccgc tcggacgacg acctcaaacg
      961 cgcttccgcc atcggcacga cgctgccgtt cgaactgacg aaggccgcaa aaagtctcaa
     1021 aatgggcctc gtcagcgacc tgaccatcca gaccgttcac ggggcgttga gggtttgcca
     1081 acacgatggt atctggctgc tggtgttcat cgggccgcgc gccaacctcg gcatgctgaa
     1141 cattgagatg cgtgccgtcc gggagctgtt tgcgtccgtt ttccaggacg atgtgaaaag
     1201 gggaatggac gcggagaagc agcgcatcct ggaggtcatt gaatccacag gggccgtgga
     1261 cagcgtcatt gacaagcgac gcggcgacct gaaagcgatg agaatgcttc acgagatggt
     1321 gttccgcatc gcgattgacg ccggcatcgg gcgggagacc atcagccgga cgatcaacga
     1381 catcaactac cggatttacc gcgattctct cgtggatatc ggttttgatt tcttcaatcg
     1441 caaagcgctg gatacctacg accccgccct tgcggagcgc gtcatggagg agcagatcaa
     1501 ggggattgcg ggtctggtga cggcgcagaa ggggaaaggc tgagcccgcg atcagccgtg
     1561 cgtcagcgac tttcctggct cgtatcgcgc cgtgcgggtg ctgcctgccg ggactgggct
     1621 tccagccgac gctcccgggc ttccagatcc gcctgaatgc gttgaagccg ccgctcccat
     1681 tcgttgagcc ggatattgcg ctgctccacc agttccatcg tgcgcttcgt gttctcccag
     1741 tatcccgcgt cctcgaaata tccccggaaa agccagtttg tgcgcagcgc ttccatattc
     1801 accgcgagtt tctgggatga gatgtcgagg ttggcgagca ccctgtccag cctggtatag
     1861 acggagtcgt cggtcaggag cctcccgacg gttccctggc cctgcatgac gttgcgggac
     1921 acgaggtcga tggtggcgcg ggtctgttcg gccgaacggg ctatggaatt ggcggcgcgg
     1981 gtgatttcgg caatggcctc ggttccacgg tcgagcatct gtttgctgct cgcgatggtg
     2041 ttgttgaggg caacataggc ggagtcatcg gtcatcagct tgccaagagt gcctcggccc
     2101 tcctgaaggt cccgcaagat ggtgttggtt gcctttgcgg ttcgccggaa ttcggaaccg
     2161 gcgtcacgca aagtatccgc cgcaacgctg taatgcgtga gtgtgcggtc gacactgcct
     2221 gcgatctccc cggcaagtcc ctggacgttc ctgagcgttc cgtccgcggt tgcggccacg
     2281 cggcgaaact gcgccaggga ggagatcacg gtatcgtaca gcgcggggtt gttgaccagc
     2341 tcgccaaggg tgccctgtcc ggcgtcaacc ttctggagga ttgacgaacc ggccgcgagc
     2401 aggtccgcgc tgttgatgac aacggcatca aggtgttcca gtgcgcggtc gatgtttgcg
     2461 ctgaaacggt gaagctggat cggcgactgg ccctgaatgg ttccgccttc cggcacttcc
     2521 ggttcgtcag gcgatcccgg cacaataatc acgatacggg cgccgagcag gccttcggtg
     2581 tcgatgatgg ctttggagtc tctcctgatg agccggcggg cgtcgccgcg gatactgaag
     2641 gtgacgacga cacggttctg cgacagctcc gggagtgcga tcttcttgac cgtgccgaca
     2701 ttgattcctg caatgcggac gaatgcaccc tcctgcaggc cgccgacgtc ttcgtaggtg
     2761 gagtagattt cgtagacatc ctcgaaaagc tgcttgcggg cgcccagaac gaacagcgca
     2821 ccgacaaaca gcgcgaaccc caggatcacg aaaactcccg tgcggatcgc cgtccctgag
     2881 ccttttttcg acgccatggt tgcctattcc tcaccaatgt ccagcggttc acgcgtgaac
     2941 tccgaggcca tgaagaactg gaccagcggt ttgtcgcaac tcctgatagt atcccaggtt
     3001 cccgacacaa gcacacttcc tctgtgcagg aaggtgacgg aatccgccac gaggcgggcg
     3061 cacggcatgt cgtgggtaac gacgataccg gtaacgccgc gttcgtcttt caggcgggct
     3121 atgagctggc tgatctcgtt cgaggtgacg ggatccagtc cggtcgtcgg ttcatcgtag
     3181 agtatgactt caggctccgt cataagcgtg cgagcgagag cacagcgttt tcgcattccg
     3241 cccgagaggt cggaagggtt cttgtcgacc gctccctcca gaccaaccca tcggagtttc
     3301 tcgtgcgcaa ggtcggtctt atcttcttca ctcaggtctt tgcgtgagcg cagcgggaag
     3361 accatgtttt cgagcacgct catggagtcg tacagcgcgc cgccctggaa gagaaagccg
     3421 acgcggctgc gccaggcctc caacgtggtc tggtccatct gcgcgacctc gactccctcg
     3481 gccagtacgg cgccttcgtc cgggtccagc aacccgacga tgcatttcag ggagacgctt
     3541 ttgccggttc cggatcttcc gaggacgacg tgcgtttctc ctttgcggac cgaaaaggtt
     3601 acgccgcgca gaacatgaag gtcgccaaac gatttgtgca ggttcacgag atcaatcatt
     3661 cgtcgttccc ggccacgcgt gtggatgggg tgctcgctgg cgtcgtttga ggattaagat
     3721 acgcaaatcg gcgggctccg gcgagggcag aagcacaccg cggaacggct gactccgcag
     3781 cagggttccg gtgcgacagg gaaggagcag tcagagggtg gcaaagcgaa cgacaatcaa
     3841 gattaccccg cgcgtaccgc gtgcgccctt ttcggcgccg ccatcgcgcg tgcatgaccg
     3901 cacgcggaga cagcagaagg tgggagagcc gtttgaggac ggcacgccgg agtttcagaa
     3961 gcccgagtat cgcttctgcc ctcgctgtgg aacgctgctg gaacctgctc ttgagcacga
     4021 cgtcgtccgt ccgacctgcc cggagtgcgg attcatcgtg tactacaatc ccgcgccggc
     4081 ggttggcgcg gttattacgc gtgagggcga gatcctgctg gtgaagcgcg cccacccgcc
     4141 gaaggaaggt ctctggagcc tgcccgccgg tttcatggag tttggagaga agcaactgga
     4201 tgcgctggcc cgggaagtgc gggaggagac gggaatcgaa atccgcgcgg caacgttgct
     4261 ttccgtggaa gacgctgcgg acgacccgcg cactcatgca ctcctgatcg cgttcatcgc
     4321 cgatgagtgg gagggcgaac cgcgcccggg agacgacgcc tctgaagtgc ggtggtggcc
     4381 ggttgacgat ctcccgcaga tggcgtggca gaaccatgtt cgcgtggtgg agaaggcgcg
     4441 ggagtggctc ggcctgtgag agttctggca gtagattacg gggataggcg tgtcgggctg
     4501 gcggtttccg acgaactcgg gattgccgcg cacggtcttc ccaccctgca ggtgaaatcg
     4561 cggaaagagg cagtgcgagc ggtcgttcag gcggcgaagg agcacggcgt ttcacgcatc
     4621 gtgatcggcc tgccgctgaa tatggacgga acggaggggc cgcgggcact ggtgacacgg
     4681 gagttcggcc ggaactgtga agatgcactg gcggcagaac ggtgcgagat tcgggtggaa
     4741 atgctggatg agcgcctgac tacgatgcgt gcgcgcgctg ctctgcgcga gcagggattg
     4801 agagagcgga gccagcgcgc gaaagtggac agagtatcgg ccacggtgct tctgcaggat
     4861 taccttgccg cacaatcggc cacagagggc aacgatgacg gatgataccc gcaaaggagc
     4921 agattgcctg agttggccca ccatcatcca cgtggcggcg acggacgtgg gatggttggt
     4981 catcctgctg ccgctcagcg ttgtgttccg tgctctcctg ctcatcgcga ccggatacta
     5041 ccgccttcgc aggcggagcc ggtttctggg gggtgcgtat gctctgggtg ttgtcggggc
     5101 gctcgcctgg cttttcgccc gcatcctgcg gctctggaga gactgatgcg gaacgatccg
     5161 gggaacccct ccccgcaggt gtcagaagga cgcgagcgag acagcggatg cacggattcc
     5221 gtcaccgtga aagcactcgc aggcctcgcg ggtgtgctgg cattgttcgc ggtggtggtg
     5281 agtgcgggat gggtggctct cgatacggta cgattcctgt acacgccgcc gcggggggca
     5341 ggtctctacc gcgtggagat tcccgccggt gcgacacccc gggagatcgg acggatactt
     5401 gaatcgcacg gaattgtccg atccgcgagg atctttgcgt acgcatcctg gcgcagagga
     5461 ttaaccaggg cgcctcgcgg cggagagtac tggctggacg ggggccagaa tgtgttccgc
     5521 gtgcttgacc agatagcgcg cagcgggcgt ccgatccggc gggtgacgat ccctgagggc
     5581 tacaccgttg cggaaattgc cggtgttgtc gaacgcacgc ttggcattcc gcgggacagc
     5641 atgctcgcgc tttcgcggga cacggcactc gtgcgacggt atgcgctgaa aggcgcgcca
     5701 tcgctcgaag ggtttctctt ccccgacacc tactttatcg attccggggt gactgcagag
     5761 cacgtcgtgc gtgtgctgac gcagcaattc caccgggtct tcacgccaga gatggtcagg
     5821 cgtgcggagc aactcggcat gacgccgctc gaggttatca cgctggcatc cgttattgag
     5881 aaggaggcgg cagttgcgtc ggaacgggcg gttatcgcgc aggtgttcta caagcgtctt
     5941 aagacaggta tggggctgga atccgacccg acggtgaagt acgcgatggg ttcagggccg
     6001 gtaaacctga gtcttcggga tatccagctg gattcccctt acaatacgta ccgttatcgc
     6061 ggcctcccgc ccggaccgat ctgcaacccc ggaaaagcgg ctctggacgc cgcgctgtat
     6121 ccggcagaca ccgactttct ctattttgtt gccaactggg atggaacgca cgcgttctcg
     6181 aggacactcg gagagcacaa cgcggcgaag gccgtgagca aacggcgtta ccgggaatgg
     6241 aaagcagcac ggggtgcggc cggcacctga cgcgggtgtt cagggaagcc ggtggaggta
     6301 tcgagtggca ggaggatttt gacgagagtc cctttgccaa gttcgctttg cagcgacaga
     6361 tggccgcttt cgccgtagag tgaggtgatc ctctcgcgga tgttgctcag gccgattcct
     6421 ttccggattg actgctcaag ttgccgcccc gtcattccga cgccgtcatc ttccacctcg
     6481 atggcaagcg ttcctgcttc cgcgtgtgcg cggattgtaa cggtgccgcc ctgttcccgc
     6541 cctcctatgc cgtgtttcac cgcgttttcc acgatgggct gcagcaacat catcggtacg
     6601 cgggcgttga cgacgtccgg cgcgatctct ttctggagac gcagccggtc ggctccgaat
     6661 ctgatgcgct cgatgttgag gtagtcctca acaaaatcga gttccgcttt cagggggcag
     6721 aggttctccc gtgagtagag cagacgccgc agaatggcag agagcttgat gattacgatg
     6781 cgggcctggt caggatgggt gcgaatgagc gaattgatgg tgctcaatgt gttgaacagg
     6841 aaatggggat tgatctggtc gcgcaacgca tccagccgcg cttctgcaag gagggcctcc
     6901 tgccggcgga gccgcgcttc catgcgcggg gtgttccaga ttttcagtga cactccgacg
     6961 gatgtgagac acatcagcac gattatgtag taggcaaccg gttcggtcgg gctgtagctg
     7021 aacaaccatc gggggtttgt ccaccgcgcg cactcggttc ggaatacctc cgctccggcg
     7081 cctacgagga gaacgacaag gcgcgggtcg agcacgcgtt ccgttctcca ccggcgccat
     7141 gtggcaaccg tatcgctgaa cggcacgggg gagaagaacc agacgctgcc ggggtctttc
     7201 aacgatgcgc gcgtgatgcc agcgctgata ccgacgatgc tgccagcgat gggggccagc
     7261 agctcgccgt gccgcagggg aaccagacca ccgataattc ctgtccacac ccccgccgcg
     7321 gtacccatga tgaagccgga aagcagggga gccacgacga gcagatccgc actggtgtac
     7381 ccgagattga tccgcgtcca cgcacctgct gccgcgatca cgccgagcac gagcccgaac
     7441 tccgttcgtt ccctggtgcc gggatgttcg tgcaggagca gggcgacgaa cttccggaaa
     7501 cggagaagca ggctcgcgac catggcgaca attgcgacat ggacgagaag cttggcgaag
     7561 aggagattgt attccgcgaa aacggcctga atccagttca gtacctcgtt cacggtcttt
     7621 tctccagacg ctggtgccgg gtgtaggagt tgggaagcag gaggcagggt atgtcgaggg
     7681 aacgggcggc ctcgtacccg acaacgctgt cttccagagc aaggcactcc tccggaaggg
     7741 ccccgcaggc gtccagcgcc gcgcggtaac acgccgggtt gtccctgctt ttttcccgtc
     7801 cagaaaccac cagatcgaaa aggtggccga gtttcgttgc ctcaagcacg gcgtgtacct
     7861 gatctccccc ggcagtggtg acgagaaccg tcttgcctct gcccgttacg gcaattccct
     7921 cgcggaaccg ttctaccagc gccacaaccg gcgacagcac acgcgcctgc agagcctttg
     7981 cgtccgaata cagaaccctc cggtgctgtt cccgcaattc ccagagttcg tccgccgaaa
     8041 tggcgaggca gtagtggcgg atcatcgccc tggcgatgtc gattcccggc ataccgacat
     8101 accgggagta aatcctcgtc tgttcctccg ggtccagatc ggttcccctg gcatccacga
     8161 ttgcacggcg aagagcttcg tctttgagcc cctccgtatc cacaagggtg ccgtccatat
     8221 cgcacaacag gtacaccggg gcgaccgtcg tgtgctcaat caccgctctt cgctcctttc
     8281 ggcgtcaagt cgcggggcag ctccacgaaa acgctttcgg gaggtgcgtg aacggactgg
     8341 tgtcgaaccg gtacgggagc gggtacccgc ggaacttcca ttgcgcgaaa tccagcaacg
     8401 cggtccagtc tgcgattcca tgctgatgcc cgccttcacg gaaatggatg ccgatgttgt
     8461 gctcgactcc gaggaagcgg aacacctccc gtgccgcgag gtatgtctgc cacgttcctt
     8521 ccgggtttgc ccagaggtcg ccgagtcctt ccgtggaaag catggcgcgg ggcgcgcacg
     8581 cggctttgag gaagtgctgg tcaaacggca tttcctgttc tctgcctatc cactgccaca
     8641 ggtcgggtcc gaaccagaac tcgaagggtc cgcggatgat cgccatggtt tccgatttcg
     8701 ggccctgcca gcggtaacat cctgcgcctc cacacccgga gccgttgggg gccgtcagag
     8761 caactcgttc gtcggttgcg cccgcaagca gcgctgcttt accgcccctg gaatgcccgg
     8821 tcaccgcgat ggcatgcgtg tccacgatgt ccagggtggt gaggaagtcg atgcaccggt
     8881 gataccccca cgcccacgcg gcaagagcgc cgaaccgcgc gtccgggtag atcgcgtaca
     8941 acccggaatt tcgttccgga agcgcaatat ccggcaccac ctctacccgg ctgaactgcg
     9001 ctgagatgaa tccgcgccgc atgatggcga ggaggatctc gtccgtcaac gtgcgccagc
     9061 acccgtctcc gttgaggatc accggaaacg gcccctcgcc cggcgggacg acgaggtcaa
     9121 gacgaatgtg aaatgcaggg tggtcttcaa gcaagatgcg gtactgggag tactgcgcat
     9181 ccaggatgcg agccattttg ttggtgctca ggcgttcccc gcgcacaccg gcaggggtcg
     9241 gcggaaggcc tccgtattcg gtatcaacaa tcgtacgcag catttcccgc cgccgttggg
     9301 gccactggtc cgggtcggtt aaccgggtgc cgtcatcgca cacgaaaggg tcgggaagct
     9361 cgatgcgcga gggggattcg gtttccaaag ggatgtctcc tgcgagaaca gagttgatca
     9421 cggttttcgg ttcgggaacg cgtacgaaat gtaaggcggg aggggagcgg aggaaaccga
     9481 aacggcgtac acagagaggc gcctcctgcg ttccggcagg aggcgccgaa gattctcgta
     9541 ccgatggtga atcagcgatt catcatcggc ctgcggccag ctccgggagc cggggcctgc
     9601 atcccctgca tggcgcacgg cggctgccat gagggatcga ggaatttggt gagttgctcc
     9661 tgggtgagga caccgcgtat ctgaatcatc tcctgagtac gcagcgtctg aatctgcgtg
     9721 cgcaggttgc cgatggtggt cagtttcgcg ttgacctgat tcatgttggg ctgggtggcc
     9781 tgaagcagtt gctgcaggtc aatctgcgcg acgcggattt cgctctgctt cgggatgatc
     9841 tgtttctgga aatcctcctg aatcctccgt acgcgagccc actggtcgga ggtcaaattc
     9901 aatgcctgca ggcgcatcag acccatacca cgccccgcca ttccggcgcc cataccgcgg
     9961 cggccgcaca tcatcgcctg accacccggt gccgccatgc ccataccggt tcccggcggg
    10021 cacatgccgg gtcccatacc cgcacccatt cccctcatgg cgaacatccc catcgcgggg
    10081 gtgccgtctt cgtcgtccgc ggccggggaa gaaggcgtct gcgcaaaaac tgccaccgcg
    10141 gcgaggctga gagccgcgag cgatccgaaa atccacaggt tctttttcac gttccaaacc
    10201 tccagaagag tagaagctgt ttaccggcgc cgtttcacaa ggatgaacgg gttggcctgg
    10261 cgctttattg tgttccgccc actactcacg gcggcggccg gaggaagacg gcgcctgccg
    10321 aactctgccg gtgcgccccc tggtttcgta tactagatga acagacaaat ggttccgtca
    10381 agggcacgtt tcgcggtgcg ggttttttga tccccgcgca agcacggacg tcatctcaaa
    10441 ggagtccctt cgtgagcaac atggaaaacc acgcgaaagt tcttatcctc gggtccggtc
    10501 ccgccgggct taccgccgct atctacgctg cgcgcgccga cctcgcgccg cttgtggttg
    10561 agggcgggca accggggggg cagcttatta tcacgacgga agtggagaac ttccccggct
    10621 ttccggaggg aatcatgggg ccggagctta tggataacat gcaccgccag gccgagcgct
    10681 tcggtgcgcg ttttgtatac ggtgtggcaa cggaggtgga tctctcacgg cggcctttcg
    10741 tggtggacgt cgagggcacg aactacacgg cggatacact gattatcagc accggggctt
    10801 cagcacgcct gctcgggttg gaatcggagg cgcggctcat ggggcacggc gtgtccgcct
    10861 gcgccacgtg cgacggtgcg ttcttccgga accagaaagt gctggtcgtg ggtggaggtg
    10921 attcggcgct ggaagaggcc aatttcctga cgaagtttgc gtcggaagtc tgcatcgtcc
    10981 accggcggga tgaactccgg ggctcgcaga tcatgcagaa gcgcgtgctc gcgaacccga
    11041 aaatacgcat gatctggaac tcggtggtgg aggacatcct cggggatccg gccaccggag
    11101 tgaccgcggc agtgctgcgc aatgtgaaga cgaatgcgct cacagaagag ccgtgccagg
    11161 gagtgttcgt ggcgataggg cactcaccga acaccgaggt attcaaggga aagctcgatc
    11221 tggacgaaaa ggggtacatc atcacaaagc cgcactcgac gaaaacaaac gtcacgggtg
    11281 tgttcgcgtg cggtgacgtg atggattcct cgtaccggca agccatcacc gctgcgggaa
    11341 ccggctgcat ggccgcgctt gacgccgagc ggttcctcgc ggagaacgag tgaacacacc
    11401 cggttgcgtc ctgttctgaa ccacccgtag ccgcggttgc ccccctttcg gggttgggca
    11461 cacccggccc ctaacccggg gcgacaacac ctcgtgacta cccccgccgg ctcgcgcctc
    11521 tgccgacggg gggcttgtgt ttcgcgctgc gcggtgtact attcgctgta ttccaccttt
    11581 cccggcgccc ttctctgttt gttcgccata gacagttcct gtcgcccggc gagacacaat
    11641 cccgcccccc ggcagagaga ggagacagac aatgcgcgcc aggattccgg gtattctctc
    11701 gatcgctctg ctgggcacgg tgggacaggc ggcagcagcg cctgcgccgg ctccgcctcc
    11761 tgtggcccgc ccggcaagag ccgcatcgga ccacgtgccg cttgcaatgc ggtggctcga
    11821 catcaacaac ttcagatgcc ccatcaccaa ttacgcgttt atcgggaaga acgaaattga
    11881 caaccgcgcc ggaggggaat ggccggcagg ttcgggcgag gagtatctgt tcggcagcgg
    11941 catcctgatc gccggaacgg ttaaccagga gcccttcacg gaaaccctcg gcgcacgcac
    12001 ggcatactcg ttccgtccct cctcgaccaa tcctctcggc tggcaggacc ttggcgcaac
    12061 cgaaagcggc gccggaagcc gcgcaattct cacggatccc cgtgctgcga tcgggtacaa
    12121 tccctccggt cgcgtgagcg agatgtcacc gttgacgaag gtgttcgtca gcgcggaacc
    12181 tgactggccg ctgggtgttc ccacttcggt actcgacacc tacaccgaat atgatgatca
    12241 ggacccgacg cgctggaata cgggtgaggg atcgaactcg ggtgccgaca ccggggaatg
    12301 gtcgggaact cctgacaaca ccaccgttgc gacgtatggc ctcggcgtga aagtgaaaca
    12361 gacgacgtac tcctggaatt acgccggcaa ttctgacatc cacttcatcg tgtttgacgt
    12421 agagaacatc cgggcggacg gaagagcgat caacaactgc ttcgttggaa tggttgccga
    12481 ccccgacatt gggagcaatg cgtcggacga catgacgggt ttcgacgcca cgcgcaacct
    12541 cggctacgca tatgacagtg atttttccga ggtcgggttt cgccgtgttc cgggtttcct
    12601 cgggtaccgt ttcctcaaaa gccccgtggc cgatcacgat atcgacagga acggcgacgg
    12661 tgtgattgac ggaaactcag tcatgatcaa cggagttccg gtgaaggacg ttcatgcggg
    12721 agaacagatc ggattgcacg cgttcaaatc gttcaaccag gccagcggtg acccagatac
    12781 cgaatgggaa tggtacatgg cgatggcggg acacaatttc cgcgaggatc aggctcagat
    12841 ttatcaaccg ttcgattttt cgaatacgcc gaacgaccag cgctttctgc agtcaaccgg
    12901 cccgttcacc ttgcaaccgg cccagccggt gcggatcgtg gttgcgatga tggtggcgaa
    12961 agccgcgggc aaccaagggg atcccattga ggtccgtgtt gcggagttgt tgcgtacatc
    13021 cgacgtcgct cagtccatct tcgacaataa cttcgtgctt ccccgtccgc cggtggcgcc
    13081 gagattgaca gcgcgggcgg gggatgggcg gatctacctc tcgtgggata ccgcggcgga
    13141 gacgaccccc gatccgttct atgcggttgc gtcaaatccg gcttcaacgc tgtacgatcc
    13201 gcggtaccgg cagtacgatt tcgaagggta ccgcgtgtgg agaagctcga ccggaaaccc
    13261 gaacgactgg cagttgctgg ccgactacga tgtcgtgtcg gcaataccgc gtgatgcggt
    13321 gataagcctg caggctggca cctacagcgg aacgctcatc tacgatgccg ctaccacgga
    13381 aggctatttc ctcagtcgcg gaggcacaga tctcttcaac ggccacgaat accttgtgat
    13441 ggtggaaggt ggcacgcaaa gggtgaaggt ctacgatgtc agcctcggag gacaggaact
    13501 accgtgcaac acttccgcgg acggcgcgga ctggtggttc tacgtcgggg acaacagcag
    13561 gtttgagttg tttgacggga atctcaatcc tctcagcagc gacccggaac gaaacgcgaa
    13621 cggcacgtac gaccagtacg ttcccggcat ggtgatgtat ctgggcggga tggcgctccg
    13681 atttgccgat gtgccggctc cagccggaac ggcgatctgg cgtgttgttt cgtccaaaga
    13741 ggagaatttc ggcgggaata cctccgttgc ccacagctac atagacggag gtctcagaaa
    13801 cggtcaactc taccattacg cagtaacggc gtatgacttc cagctttctt ctccgaggag
    13861 ccttgagagc gcgaagtccc tgagtatgac gtcgatttcg cctcgtcacg atccttcgga
    13921 taccacaagg gttaccaccg ccgtccacac gaacggagtg gccgactgcg ccgtcaacgt
    13981 gcaggtggta gacccgatgg ctctcaccgg gcacacgtat cagatagggt tcaaccccga
    14041 cggcacgtgg tttgtgcgcg acacaaatct gcccgccggc caggacattg tcatcaacaa
    14101 ccagaccaat ctttccggcg atctgaatta ccccattgtt gacggagttc tcgtgaacgt
    14161 gatcgctccg cccgtcggca tcaaatccac ctcgtactcc ccgtcggaaa acaaatggtt
    14221 cgtcgaacgc tcggcaggct atgaggcgtt gaccggccag gaatgggggt ggggaacgac
    14281 agttacggca aaggactacg cacgagtgga gattcgcttc aatggagggt caactctcgc
    14341 gccggtgtat ctgcgcggcg gcacaccgaa ctattcgtat gtcggagccg gcactatccc
    14401 tgccgaaatc tgggatgtga ccaataaccg tcgcctcgcc gccgcgttcg ttgagcaaaa
    14461 tggcgcggtc tcccgcgacg gaaactggat gccagccaca gcggcggacg gcgggcgcga
    14521 atacctgttt gttctggcgc aaccgtacaa cccggatact ctgtcggcgg attgggactt
    14581 tttccggagc aagagcattc ttgccgatgc gtcgcagttc ccgctgatga cgggaagctg
    14641 gatcattgcc aaagaaggta tggcaccggc aacgggcgac gtatgggcga tttcgcctta
    14701 cctcgggaat acgccggagg acgtgtttga gtacaccacg acgcatgcgc cggtcgcaag
    14761 ctcgctcgag cttccgatcc ttgccgcggg acgccgtgag gtggtgacgt tccccgttag
    14821 ggggacgcta tggaacgcgc gttcggcgca gatgatgttt cttgtgggcg cgaatacggt
    14881 tcaaagcgtc tcgattgtgc accatgcgtt ccaagggcaa caaggcgcgc aggccgtcgc
    14941 caccctcgcc ggtgacacgg tgcgagtggc catgtcaggg gagcaggagg tttccctgag
    15001 caatcaggct cttgtggtgt tgagcatgac gattcgtgcg gacgctgaca ccggaatcac
    15061 gcaccttgtc tggttgccgg ccccgttgac cagtgtggat gaggaaggcg tgaatcttgt
    15121 gaacggggag ctgtacgtat cgttcatccg gtacggcgac gtcactgcag acggctcgct
    15181 cacggcgcag gatgcaacat ggatccttca gtcggtcgtc ggcattcgaa ccggggtgga
    15241 tgtggaaacc gccgatgtgt cggacaatgg ttcaataacc gcgtacgacg cagctctcgt
    15301 gctcttcaag gtaattcacc ccgaatacgt gttcccggtg cgcggcgggc cgcttatcaa
    15361 gccttcggcg cgcgctccgg tttcgctggt ctgggagcgg accgacgcgg gatggtcgct
    15421 tgttgtggag gatgcgagcg ggatcctggc cggagacgtg gcgcttgcgc tgccggacga
    15481 agtgcaggtt caggttgcgg ctgacggcgc attcgcatac cggcaggatc acaggacgct
    15541 gtacgtttcc ttcgtgcatt cggcgggtga cgaccgcatt ctgttcaccg ttctcacctc
    15601 tgccatggag actccggcgc ttaccgctgc gtcgctgaac gaaggcgcaa tactgttgaa
    15661 gttcgcgca
//
LOCUS       DAOTNG010000031        14977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_3920_length_14977_cov_5.594960, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000031 DAOTNG010000000
VERSION     DAOTNG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14977
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_14977_cov_5.594960"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="P5179_02685"
     CDS             <1..1075
                     /locus_tag="P5179_02685"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS94157.1"
                     /translation="TRRAQDQADVASAEGARCAPPYARKKPEAMRRTLPTPGERRFTR
                     GVVLALSDITSWNWPEEASYAGLTTIATHGMAGDSAEFMQSEVGEELRTRCERVGVEI
                     EHELHAARELLPRELFARHPDMFRMNEAGDRVPDANLCVHSEEALEVCCQNAAALARA
                     LAPTTGRHFFWIDDGQPMCRCSRCRPYSDSDQALILENRLLTTLRKDNPKATLAHLAY
                     ANTLWPPADVKPLPGVFLEFAPIQRRYDLPFGVRDARPGGEKAPGHGEQLDALDANLE
                     LFGAEGAQALEYWLDVSRFSGWNRANVARIPWDKRIVEADAKLYHDRGVRNVTTFAVW
                     LDGDYVRRFGTPPLREYGDALRW"
     gene            1069..2247
                     /locus_tag="P5179_02690"
     CDS             1069..2247
                     /locus_tag="P5179_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS94158.1"
                     /translation="MVEIPAKTVWIDRRIADHPECRRRLERVLPHVRCQDIRYCDGNP
                     GEEFFSVSKRRHGKDDFSDDAVLAFTTYDSGRRGFYFHWRDEAAAHGGACQPAMELNI
                     VEGCVFRCAYCGFGRRVHFTLDVERLIGELEAVFAKHPEQRLYKFSNMTDLPAFEPEI
                     DIIPPMVRRFAQETDRYLMLFTKSDNVAFLRDIDHRGHTIISWSLTADSASRLLDKRT
                     ATMDERIAAMAEMQKAGYIVRARLSPIIPVRNWEKEYRDLFEHMFACVQPDLVTLELL
                     GWMKFDDLLAVIPPELLDGQMCEEARAAAESLKDVMWAPFTQDAHEKVYRFCIDTVRE
                     LSPRTPVSVCHGTAATWKALGELTGMTPGEYICNCGPLSAPGGALYDELHALHDGEGQ
                     "
     gene            2244..>2853
                     /locus_tag="P5179_02695"
     CDS             2244..>2853
                     /locus_tag="P5179_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS94159.1"
                     /translation="MNERFVQLDRGDGTRVTARVRAGKGPNLILVPGTWGNMDTRGPL
                     MERLDPELNIVNVALPGQDDNWPPPKDPSIPAFTADVLRLADHLGLERFFVGGNSLGG
                     MISIDMIRLAEQRLLGVITLEGWTHWSALYDAFGDNTEVTLNPQQSAFLQEVRRKLLD
                     RWDPGMRSRYATMWREWDGSETLQNCTVPLLSIWGDRGKPRPA"
     assembly_gap    2854..2953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3006..5315
                     /locus_tag="P5179_02700"
     CDS             3006..5315
                     /locus_tag="P5179_02700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HRS94160.1"
                     /translation="MTSSVEGVSMPLFPTDLPEREWCQFNAEGFARPVCGLIHRGTNA
                     PVCGMPLGGIDTGCLDLEATGVWGYCSIFNSLNPRRGAINEPFLGVSVGRQTWVLSTV
                     ALSRRDGQEWIDTMRSWANYRAVKSASDIHYWGHFPVADLEYETDSPLSVGMRAWAPF
                     IPGDVALTNAPAAVFEVHLRNSTETEQKGTLAFSFPGPNEGEAGTTTFRRVKIEGAAT
                     GVSVESPQASYVLTAIGESSVRTGRCLGTDAVAWSCIEDALPFVEAGAGTSIAVDFAL
                     SPGAEKTIRFVLAWFAPVWNGGGIMTGKGQDYRHMYARRYANARAVAEMITGRHEEIL
                     RRIIAWQSVLYGDEGYLVWLRDALVNVLHLIPETSIWAQAKPPVGDWCNEEDGVFGMN
                     ESPRACPQIECIPCSFYGNFPLVYFFPEAALSTLRAYKAYQYPSGQAPWVFGGCTVGS
                     PPYEMAMPSPGYAKKPQTTLDGGCYVDMVDRMWRRTGDDQLLREFYDSVKRNTIFTMN
                     LRPGSGAAGIVSMPEGNQAQDWFESCDLFGIVPHIGGVHLAQIRMARRMAKAVGDEEF
                     VRQCDSWLAQGSEVLENETWAGTHYLLFKEKETGKESDIIMSYVFDGEWMAKLHGLEG
                     VFRPDRVKTMLETIRRTAIAMSPFGSVVFCKPEGALGKEDWDPGYWGAHGVHPPGTFM
                     LAMTYLFYGEKDTGLDLARRTMGEIVKRGWYWDIPVAIDGTAPRIGFDYYQNLVLWSF
                     PAVMEGADIAAPCKHGGLVDRVIKAGAEA"
     gene            5439..6719
                     /locus_tag="P5179_02705"
     CDS             5439..6719
                     /locus_tag="P5179_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase-like family protein"
                     /protein_id="HRS94161.1"
                     /translation="MATAGTPVHAAATLPVHLPAKLSICCWIWSWITSATEGEPYHDL
                     DRCMAGLKERGFNAVRVEAGLNWAFRADGSPRGQVAFRPWIAGYGWNFSTVNSRGGGR
                     HDVLQRLGALFESARRHGIYVILTSWEYQDSTSFLADPALRAEILAVPPGNRYMHLAK
                     LHDCLLTVLEERGLEQNVAFVEVHNEPDASEFPGGEEGRELHEEAIGFLRDRHPEVLI
                     TGDFTSHDYAIVPENVQVFDQHVYAGFGWYFRTLFGETVLNKDFDPGKPRALPALNRV
                     LRRDLVPWSEFMVAAENVREFWRPIMWMYENLDNEKWDEWVAETFSEWAPRIREKAET
                     AFASDAREGRRRGLPLVFDEGGYFYPPRLARFEVFGEGLSLFELMCDLAITHGYWGFM
                     PGTYCGPEHLAWQENPAWLLGINTRFQKGILSGS"
     gene            complement(6780..8090)
                     /locus_tag="P5179_02710"
     CDS             complement(6780..8090)
                     /locus_tag="P5179_02710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS94162.1"
                     /translation="MNTNPFHSLGLDDRILRALVDQSYETPTPVQLDSIPRVLEGRDL
                     LATAQTGTGKTAAFCLPMLHLLNTREGRSNKHVRALILTPTRELALQIGESLRAYGRY
                     LPLRFTVLLGGVAQAPQAKALREIPDIVVATPGRLLDLFGQRLIQLDRLEILVLDEAD
                     RMLDMGFVRDVRKIVSATPATRQTLFFSATLSPEIAELASDMVKNPAMVAVTPVASVS
                     DHIAQKVLFVEQTDKLSLLTEILREDSVSRALVFTRTKHGADRIVRQLERKGIRAEAI
                     HSNKSQNARQTALAAFDRGRTNVLVATDIVARGIDVDGITHVINFDLPNEPEAYVHRI
                     GRTARAGAVGTALSFCGEDEVSLLFAIEKLTTCPLSDVDGHAFHSTAISAMRDRKRAA
                     QQGTGGSSRFGSGRSGRNRPLSSQRRRYHPTAGPATGARVSHAA"
     gene            8526..11768
                     /locus_tag="P5179_02715"
     CDS             8526..11768
                     /locus_tag="P5179_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94163.1"
                     /translation="MHRMTIPWITTAQTTRGGRGGTTTAERTIALKCDLPDEPALRVT
                     GELTLARPEREATLSVGSSVCTLGVSGDASDPYLLHARAWVSDPDSPRGTVAYRLVPD
                     VTRWAHDEAGREQYRREFAHAKGWAEHRIRLSIDITPCEVRAWVDGRLVASWSARNPQ
                     PGVALVHLPEGARPHGAAEVTILPGEEEFLPLDLHPFARGEDFLNLKETAYRGRAPRV
                     FTSSLSSDPKRALLRIPRKTYDRVHLVCSASTEGGTLPSASVAFIKPERGHTVFTEFL
                     VNPPYHTPKTITVPLDPCAFSDFLNDPGEEALEVEFCQRMDYDEGRFPHPAGPPSGVR
                     IHEITFETAPVALRVTSSAVGHVFQGTKTPRFFVHLESQRSVPGTVRVAVEVAEPYGG
                     KSKSSARVRLAPWEKRTQTFELPQRMYGRFGLTVTVSQEDGSRPLKRSTTFALLPRDT
                     RKATSDSPFGIWCFFENHHGVPPEIAAPLFRKAGARWTLANFVVRDDPDETRKRVQTL
                     AAHGVGLACANVACIGNTCNAGGNDVDAMIRRMHAMPPVKHWLLFWETFLSRQHMRDY
                     PFDILGRPVRELTPDEESVFKNCWDTAIEYATRVRKEFPEAKLIFGNGHPNFIAAFLR
                     RGFPKEYIDGFGLDFDMFVSSPELQPGPLHCPFSGLFFLKTLQDLYGYSEIPRYLTEA
                     IYCSQSEGWLTERQQADHYVRSHLLGLASGVVLFGMTAELNDPGSEYFYGSYGPVGFL
                     RRAPELNPRESFCAYATMTRMLDRANFTEMVPMPSPGTYGLRFTKADGSDIYAFWTTT
                     GTRLLTFGAESGSVRTTDLFGNQTVIRNARGVFSLNVSPSPVYVEGEGIHGFVLGTPR
                     HRPGPKTSEPIVRMGDLPSWRHEAEDDAWMERLGRAGNWAITAPTIRGRFEFSLRKDR
                     ARGQVLRVSLSETPGAHPLHLRYAVLRFSRKVPVNGAFSALGLWIRGNSSSGRVLYEL
                     EDADGIIWRSVYSDTYIDFDGWRYVQTPLPLPATEDGSRLQPCGLTPWATETEHRHPA
                     LPVRLTALIIESRTHVVRATSLDPVPDTTFDVSDVVVTKEMVDPEIRFRW"
     gene            11837..12814
                     /gene="miaA"
                     /locus_tag="P5179_02720"
     CDS             11837..12814
                     /gene="miaA"
                     /locus_tag="P5179_02720"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRS94164.1"
                     /translation="MCTGSSHPAEDNALPVIPIILGPTGSGKTALVVDLVRDGRLTGI
                     EVVSADSRQVYRFLDIGTAKPTEEERHTVPHNLVDILDPSETYSAGRFRRDAEAVIAE
                     ILKRGSTPVVVGGTGLYLKALVDGLSPIPRVPVDVVDRLRAEIASAGPAALHERLQEL
                     DSRCAATIQPTDTQRIIRALAVHEATGKPLSRWWEEHPEKPPYRYVWLGIRWDRSQLR
                     DRIARRTRAMIARGLEREVRWLLDRGYTWEMNAMATVGYREWKHCFAGEITREEVTAA
                     IAIHTAQYAKRQMTWFRTNPRITWVDGSSPSLKDEVVRWLDDRHAEAKR"
     gene            complement(12863..13294)
                     /locus_tag="P5179_02725"
     CDS             complement(12863..13294)
                     /locus_tag="P5179_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRS94165.1"
                     /translation="MAMVPTLPSIQNEINRLFDALSSRFGEFSGETLSDWAPRVDVIE
                     DADNLRIYADLPGLNREDIQLSVERNTLTITGVRKPMEDANKFSWHRAERLYGTYKRV
                     FNLPTVVDPDKVSAEYRNGVLEITLPKAEHAKPKMITVKTE"
     gene            13643..>14977
                     /gene="ftsH"
                     /locus_tag="P5179_02730"
     CDS             13643..>14977
                     /gene="ftsH"
                     /locus_tag="P5179_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRS94166.1"
                     /translation="MAFERRTQLSLWYFVGAFMILLALNLYFSKSQVEPLNYSVFRQY
                     LKQGRIVEATLTAQHITGAMRTGTSTSTLARFVTVRVTDPELLDLLTQHGVIFRGRVE
                     DTWVTEMLAWIGGMAVFFVIWMFLIRRMGGPSSGMMSIGKSKAKIYVETETKVTFADV
                     AGIDEAEEEVREVVEFLKEPERFRKLGGHIPKGVLLVGPPGTGKTLLARAVAGESGVP
                     FFSLSGSDFVEMFVGVGAARVRDLFQQAKERAPCIVFIDELDALGKARGMNPLGSHDE
                     REQTLNQLLVEMDGFDANVSIIIMAATNRPEILDMALLRPGRFDRQIVVDRPDLNGRL
                     EILKIHAKRVKLDPEVDLRQLAAGTPGMVGADLANVVNEAALLAARRGHDAISQNDLQ
                     EAVERTLVGLEKKSRVLNERERKIVAYHEAGHALAGLCSPHATPVHRVTIIPR"
BASE COUNT         2968 a         4321 c         4606 g         2982 t
ORIGIN      
        1 cacccgacgc gcgcaagacc aggctgacgt ggcgagtgcc gagggtgccc gttgtgcacc
       61 gccgtacgcg agaaagaagc cggaggcgat gagacgaacc ttgccgacgc cgggggaaag
      121 gcgcttcacc cggggggtgg tgctcgcgct ttccgacatt acgagctgga attggccgga
      181 agaggcttcc tacgctggcc ttacgacgat tgccacgcac ggtatggcgg gcgacagtgc
      241 cgaattcatg caatcggagg tgggtgaaga actgaggacg cgctgtgaaa gagtgggtgt
      301 cgaaatcgag catgaactcc atgcggcacg cgaactcctc ccacgcgaac ttttcgcccg
      361 gcaccccgat atgtttcgca tgaatgaagc gggtgaccgt gttccggatg ccaacctctg
      421 cgtccattcg gaagaagcgc tcgaggtgtg ttgtcagaac gcggcggcac tggcccgggc
      481 gctggctccg acgaccggac gtcatttctt ctggattgat gacgggcagc ccatgtgccg
      541 ttgttcccga tgccgaccat actctgacag cgaccaggcg ctcatcctgg aaaaccggtt
      601 gctcacgaca cttcggaagg acaatccgaa agcgacgctg gcgcatctgg cttatgcgaa
      661 cacgctctgg cctccggcag acgtgaagcc gcttcctggc gtgtttctgg aatttgcacc
      721 catccagagg cgttacgacc tcccattcgg tgtgcgggat gcgcgacctg gcggggagaa
      781 ggcaccaggc cacggcgaac aactggacgc cctcgacgca aaccttgaac ttttcggtgc
      841 ggaaggagcg caggcgcttg aatactggct tgacgtttcg cgcttctccg ggtggaatcg
      901 ggcaaatgtc gcacgaatac cctgggacaa acgaattgtg gaagcggacg cgaaactgta
      961 tcacgacaga ggcgtacgca acgttacgac gttcgccgtg tggttggatg gagattatgt
     1021 ccgaagattc ggcacgccgc cgttacgtga atatggagat gcgttgaggt ggtagaaata
     1081 cccgcgaaaa ctgtgtggat tgatcgccgc atcgcggacc acccggagtg ccggcgccgg
     1141 ttggagcggg tgttgccaca cgttcgttgt caggatatcc gctactgcga tggaaatccg
     1201 ggtgaggagt ttttctcagt ctcgaaacgc cggcacggga aagacgactt cagtgacgat
     1261 gcggttctgg cgttcaccac gtacgactcc ggtcgacgcg gcttctattt ccactggcgc
     1321 gacgaagcgg cggcgcacgg cggtgcgtgc cagccggcaa tggaactcaa catcgttgaa
     1381 gggtgcgtct tccgttgcgc ctattgcggt ttcggtcgac gggttcattt cacgcttgac
     1441 gtggaacggc tgattggcga actggaagca gtttttgcga agcacccgga acagcggctt
     1501 tacaaattca gcaatatgac ggatcttccg gccttcgaac cggaaataga catcattccg
     1561 cccatggtcc gcaggtttgc gcaggagaca gatcgctatc tcatgctgtt tacgaaaagc
     1621 gacaatgtgg cgtttctgcg ggatatcgac caccgcggcc acacaatcat aagctggtcg
     1681 cttaccgcag attccgcgag caggctgctg gacaaacgta ctgcgaccat ggacgaacgg
     1741 atcgcggcga tggcggaaat gcaaaaggcg gggtatatcg ttcgcgcacg gttgagcccc
     1801 atcatacctg tgcggaactg ggaaaaggag taccgtgatc tttttgagca tatgttcgca
     1861 tgcgttcagc cggatctggt gacgctggaa ctactggggt ggatgaagtt cgatgatctt
     1921 ctcgccgtga ttcccccgga gcttctcgac gggcaaatgt gtgaggaagc ccgtgcagcc
     1981 gctgaaagcc tcaaggatgt gatgtgggct ccgttcacac aggatgctca cgaaaaagtg
     2041 taccggttct gcatcgatac ggtcagagag ctgtccccgc gcacgccggt aagcgtgtgc
     2101 cacggcaccg ccgccacgtg gaaggcactg ggcgaattga caggaatgac gcccggtgag
     2161 tacatctgca attgcgggcc gttatccgcg cccgggggcg ctctgtacga cgaactccac
     2221 gcacttcatg acggagaagg gcaatgaacg agcgttttgt ccaacttgat cgtggcgatg
     2281 gaacccgtgt tacggcgcga gtccgcgcag gtaagggccc gaatctgatt ctcgttcccg
     2341 gcacgtgggg gaacatggac acgcgagggc cattgatgga gcggctcgat cccgaactca
     2401 acattgtcaa cgtcgcgctg ccagggcagg acgacaactg gccgccgccg aaagacccgt
     2461 ccatccccgc cttcacggcg gacgtgctcc gcctcgcgga tcatctcgga ctggagcgct
     2521 tctttgtcgg tggaaacagc ctcggcggga tgatttccat cgacatgatc cgcctcgccg
     2581 agcaacgtct gctcggtgtc atcacgcttg agggatggac gcactggagt gctctttacg
     2641 acgcattcgg ggataacacg gaggtcacgc tgaacccgca gcagtcagcc ttcctgcagg
     2701 aggtcaggcg gaagctgctc gaccggtggg accccggcat gcgctcgcgg tatgcgacga
     2761 tgtggcgcga gtgggacggg agcgaaacat tgcaaaactg caccgttccc ttgctctcca
     2821 tctggggaga ccgcgggaag ccgcgcccgg cacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnatctcgc acgcggggaa tagcaacata cgtgtgcccg tgtggagttt
     3001 ccgtaatgac ttcatctgtg gagggtgttt ccatgccgtt gtttccgacc gacctccctg
     3061 agcgcgaatg gtgccagttc aacgcagagg gtttcgcccg ccccgtgtgc ggcctgatcc
     3121 accgcgggac caacgctccc gtgtgcggaa tgcccctcgg cggaattgat accggttgtc
     3181 ttgacctcga ggccaccggc gtatggggct attgctcaat cttcaactcg ttgaacccgc
     3241 ggcggggagc catcaacgag ccattcctcg gggtaagcgt ggggaggcag acgtgggttc
     3301 tcagcacggt ggcgctttcc cggcgcgacg ggcaggaatg gatcgacacg atgcgttcct
     3361 gggccaatta ccgggccgta aagagtgcct cggacattca ctactgggga cattttccgg
     3421 tcgctgatct ggaatacgag acggactccc cgctctctgt ggggatgcgc gcgtgggccc
     3481 cgttcatacc cggcgacgta gccttgacga acgcgccggc cgcggtgttc gaggtgcatc
     3541 tgcgcaactc tacggaaacc gagcagaagg gcaccctggc gttctcgttc cccggtccga
     3601 acgaaggcga agcgggcacc accacctttc ggcgggtcaa gatcgaaggc gccgccaccg
     3661 gggtgtcagt tgagtcgcca caggcatcgt acgtcctgac cgcaataggt gaatcctccg
     3721 tgcggacagg tcgctgcctc ggcactgatg ctgtcgcgtg gagttgcata gaagatgccc
     3781 ttccgttcgt cgaagccggc gcaggaacga gtatcgccgt ggatttcgcg ctttcgcccg
     3841 gcgcggagaa aacgatacgc ttcgttctcg cgtggtttgc gcccgtctgg aacggcggtg
     3901 gaataatgac cggaaagggg caggattacc gtcacatgta cgcccgccgg tacgcaaatg
     3961 cccgtgccgt tgccgaaatg atcaccggtc gccacgagga aattctgcgg aggatcatcg
     4021 cgtggcaaag tgtgctctac ggcgatgaag ggtaccttgt ctggttacgc gatgcgctgg
     4081 tcaacgttct tcacctgata ccggaaacat ccatctgggc gcaggcaaag cccccggtcg
     4141 gcgactggtg caacgaggaa gacggggtct tcgggatgaa cgaaagcccg cgggcctgcc
     4201 cgcagatcga gtgcataccg tgcagtttct acggcaactt tccgctcgtg tatttctttc
     4261 ctgaagccgc actttccacg ttgcgcgcgt acaaggcgta ccaatacccc agtggccagg
     4321 ccccgtgggt atttggagga tgcaccgtcg ggtctccccc gtacgaaatg gccatgccgt
     4381 cccccggata cgcaaaaaaa ccgcagacga cgctcgacgg cggatgctac gtggatatgg
     4441 ttgaccggat gtggcgccgg accggagacg accagcttct ccgcgagttc tacgactctg
     4501 tcaaacgcaa cacgatcttc accatgaatc tgcggcccgg ctccggcgcc gcggggatcg
     4561 tcagcatgcc ggaggggaac caggcacagg actggtttga aagctgcgat ctgttcggga
     4621 tcgttcctca catcggtggg gtgcatctcg ctcagattcg catggcgcgc cggatggcca
     4681 aggccgttgg cgacgaggaa ttcgtgcgcc agtgcgattc gtggctcgct cagggaagcg
     4741 aggtccttga aaacgagaca tgggcaggaa cgcactatct cctgttcaag gaaaaggaga
     4801 ccgggaagga atccgacatc attatgagtt acgtgttcga cggcgagtgg atggcaaaac
     4861 tccacggatt ggaaggagtc tttcgccccg atcgagtgaa gacgatgttg gaaactatcc
     4921 gacggaccgc aatcgccatg tcgccgttcg gctcggtggt gttctgcaaa ccggaaggtg
     4981 cgctgggcaa ggaagactgg gatcccggct actggggcgc ccacggtgtt cacccgcccg
     5041 gcacgttcat gctggcaatg acgtatctct tctatggcga aaaagatacc ggccttgatc
     5101 tcgctcgccg gaccatgggc gaaatagtga aacgcggatg gtactgggat attccggtcg
     5161 caattgacgg cacggctccc cggatcggat tcgattacta ccagaacctc gtgctctggt
     5221 cgtttcccgc ggtcatggaa ggcgcggaca tcgcggcgcc gtgcaaacac ggcgggctgg
     5281 ttgaccgcgt catcaaagcc ggtgcggagg cgtgatcgcg ttataatcgt cttcagggcc
     5341 tgtgaactga cgctgaccgc tcctttgaac ctgttgcaga cacctttgcc tttgcgctga
     5401 aggtctgcgc acgtgcatga cgcgaaaagg aaaccacgat ggcaactgca ggcacgccgg
     5461 ttcacgccgc tgcgactctt cccgtgcatc ttcccgcgaa gctttccatt tgctgctgga
     5521 tatggagctg gatcacctcg gcaacggaag gcgagccgta ccatgacctc gacagatgca
     5581 tggcgggtct caaagagcgg ggattcaacg cggtgcgtgt ggaggccggc ctgaactggg
     5641 cattcagggc ggatggttca cctcgcgggc aagttgcgtt ccgtccgtgg attgcaggtt
     5701 acggctggaa tttcagcacc gtgaactcgc gcgggggcgg tcgtcacgat gttctgcaac
     5761 gcctcggggc gctgtttgaa agcgcgcgaa ggcatggcat atatgtgatc cttacgagct
     5821 gggagtatca ggacagcacc tcgtttctgg ccgacccggc acttcgcgcg gaaatccttg
     5881 cggtgccgcc gggaaaccgg tacatgcacc tcgcgaagct gcatgactgt ctcctcacgg
     5941 tgctggaaga acgcgggctg gagcagaatg tcgcatttgt ggaagttcac aacgaaccgg
     6001 acgccagcga gtttcccggt ggagaggaag gaagggaact tcacgaagaa gcgataggat
     6061 ttctgcggga ccgccacccc gaagtcctca ttaccggcga tttcacctca cacgactacg
     6121 ccatcgttcc ggagaacgtg caggtgtttg atcagcacgt gtatgccggg ttcgggtggt
     6181 atttccggac cctttttggc gagactgtgc tgaacaagga tttcgacccc ggcaaaccgc
     6241 gcgctctccc ggctctcaac cgcgtactcc gaagggacct cgtcccgtgg agcgagttca
     6301 tggtggctgc cgaaaacgtg cgcgagttct ggcgccccat catgtggatg tacgaaaacc
     6361 tggataacga gaaatgggat gaatgggttg cggaaacgtt ttccgaatgg gcgccccgga
     6421 tacgcgaaaa agcggagacg gcgttcgcct cggatgcacg ggaaggcaga cgccggggcc
     6481 tgccgctggt gttcgatgaa ggcgggtatt tctatcctcc ccgactggcg cgtttcgagg
     6541 tgttcgggga aggtctttcg ctttttgagc tcatgtgcga tctggcgatc acccacgggt
     6601 actgggggtt catgccgggc acgtattgcg ggccggagca cctcgcctgg caggagaatc
     6661 ccgcgtggct tctcggcatc aacaccagat tccagaaagg gattctttcc ggttcctgaa
     6721 agaaaacgcg tcgcctgaag agactcaggc gacgcgcatc ttcgaaactc agtggtcatc
     6781 tacgccgcgt gcgataccct cgccccggtc gctggcccag cggtcgggtg atagcgacgc
     6841 cgctgggaac tcaacggtcg gtttctcccc gaccttccgc ttccgaatct cgaactgccg
     6901 ccggtaccct gttgagccgc acgcttgcgg tcccgcatgg ccgaaattgc cgtggaatgg
     6961 aatgcatgcc cgtccacatc agaaagcggg caggtggtaa gcttttctat tgcgaagagg
     7021 agtgaaacct cgtcctcccc gcagaacgaa agtgcggttc caacggcacc ggcgcgggcg
     7081 gttcgcccta tgcggtgcac gtaggcttcc ggctcgttcg gcagatcaaa gtttatcacg
     7141 tgcgtgattc cgtccacgtc aattccgcgg gcgacaatgt ccgtagcgac aagcacgttc
     7201 gtccttccgc ggtcgaacgc ggcaagcgcg gtctgccggg cattctgact cttgttcgaa
     7261 tgaattgcct cggcgcggat gcccttgcgc tccaactggc ggacgatacg atccgcgcca
     7321 tgcttcgtac gggtgaacac gagcgctctg ctcacagaat cctcacgcag aatctccgtc
     7381 agcagggaaa gcttatccgt ctgctccacg aagagaacct tctgcgcgat gtggtccgaa
     7441 acggatgcca ccggcgtcac ggccaccatc gccgggtttt tcaccatgtc cgacgcaagt
     7501 tcggcaattt ccggcgacag tgtggcggag aaaaacagcg tctgccgggt tgcgggggtc
     7561 gcgctcacga tcttccgaac gtcgcggaca aatcccatgt cgagcatcct gtccgcttca
     7621 tccagaacca gaatttccag ccgatcaagc tgaatcaggc gttgcccgaa aagatcgagc
     7681 aatctgcctg gagttgcgac gactatgtcg gggatctccc gaagtgcttt cgcctgtggt
     7741 gcctgtgcca ctccgccgag caacacggtg aaccgcaaag ggagatatct gccgtaggcg
     7801 cgcaggcttt cgccgatctg gagcgcgagt tcccgtgtag gggtcagaat gagggcgcgc
     7861 acatgcttgt tgctccgacc ttcgcgtgtg ttcagaaggt gaagcatcgg caggcagaat
     7921 gccgcggttt tgcctgttcc tgtctgggcg gtcgcaagca gatcgcggcc ctcaagaaca
     7981 cgcgggatgc tgtccagttg aacgggtgtg ggggtctcgt agctctggtc caccagtgcg
     8041 cggaggattc gatcgtcgag accgagtgaa tggaatggat tggtgttcat gaaaaactcc
     8101 tgtctttctg taccgagacc agcggcgact aactctcggt gatacagtca gaacaggccg
     8161 gcgatcgtca agaggggttg acgggggcgg gcatgtgagc cgagaaataa cgcagacaat
     8221 cttcggaagg tttgctgggc accggtattg tcccgtatgg actgattccc gatacccgct
     8281 tttcaaatat acacaaaatc gcgatttatg caaccatggg ctgggacggt accctttcgg
     8341 agccgccacg gtactgcccg gaagctccgc tcaggcgtgt tcctggagcg ccggtggatc
     8401 atgatccctc gcaggaaacg ccggcaaggg cgacggaggc ttttgccttt gaggcgctca
     8461 cgctgcgtgc gatacttctt caagcccgaa acgcgtcttc ctgactcgcg acattggagt
     8521 gcaccatgca tcgcatgaca attccatgga taacgacagc acaaacaact cgcggcggtc
     8581 gtggagggac aaccacggcg gaacgcacca tcgcgctgaa atgtgacctt ccggatgaac
     8641 ccgcgctgcg cgttacgggt gaattgacgc ttgcgcgccc ggaacgcgag gccacccttt
     8701 cggtcgggtc atccgtgtgc acgctgggag tgagcggcga cgcatccgac ccgtacctcc
     8761 tgcacgcacg ggcatgggtt tcggatccag attccccccg cggtaccgtg gcataccgcc
     8821 ttgttcccga tgtcacgcgt tgggctcatg acgaagcggg ccgcgagcag taccgacggg
     8881 aatttgcaca tgcgaaggga tgggccgaac acagaattcg gctctccatt gacatcacgc
     8941 cctgcgaggt tcgtgcgtgg gttgacggcc ggctcgtggc ttcgtggagc gctcggaacc
     9001 cgcaacccgg tgttgccttg gtgcaccttc ccgaaggcgc gcgtccgcac ggtgcggctg
     9061 aggtaacgat cctcccgggt gaagaggagt tcttgcccct tgatctgcac ccgtttgcgc
     9121 gaggcgaaga cttcttgaac ctgaaggaaa cggcgtatcg cggtcgcgca cctcgtgtgt
     9181 ttacctcctc tctgtcgtct gatccaaaac gcgccctcct ccgcataccg aggaagacgt
     9241 acgatcgcgt gcacctcgtg tgctctgcca gcactgaggg gggtactctg ccctccgcat
     9301 cggtggcgtt catcaaaccg gaacgcggcc ataccgtgtt taccgaattc ttggtgaatc
     9361 ctccgtatca cacgccgaag acaatcacgg tacctctcga tccatgcgcg ttcagcgatt
     9421 tcctcaacga ccccggcgag gaagcgctgg aggttgagtt ctgccagagg atggattacg
     9481 acgaaggccg ttttccccat cccgccggcc cgccatccgg cgtccggatt cacgagataa
     9541 cattcgaaac cgcacccgtt gcgctccgtg ttacctcctc cgcggtgggg catgtgtttc
     9601 aggggaccaa aactcctcgt ttctttgttc acctcgagag ccagcggagt gttcccggga
     9661 cggtgcgcgt cgcggttgag gttgcggaac cgtatggcgg gaagtcaaaa agctcagcca
     9721 gagtcaggct ggcgccgtgg gaaaaacgaa cacagacatt tgaactgcct caacgcatgt
     9781 acggccgttt cgggctcacg gtcaccgtct ctcaggaaga tgggtcgcgg ccgctgaagc
     9841 gttcgaccac attcgccctt ctgccgagag acacgcggaa ggccacctca gactcacctt
     9901 ttggcatatg gtgcttcttc gaaaaccacc acggggtgcc accagaaatc gccgctccgc
     9961 ttttccgaaa agcgggtgcc cgttggacgc tggccaactt cgtggtgcgc gacgatccgg
    10021 acgaaacccg aaaacgcgtg cagacgctcg cggctcacgg tgttggcctt gcctgtgcga
    10081 acgttgcgtg tatcggcaac acatgtaacg ccggcggaaa cgacgtagac gccatgattc
    10141 gccgcatgca tgcgatgccc ccggtcaagc actggttgct gttctgggag accttcctca
    10201 gccgccagca catgcgcgac tatccttttg acattctcgg caggccagtc cgtgagctca
    10261 ctcccgatga ggagagcgtg ttcaagaatt gctgggatac ggcgattgag tacgccacac
    10321 gcgtgagaaa ggaattcccg gaggcaaagc tcatcttcgg gaacgggcat ccgaacttca
    10381 ttgcagcgtt cctgcggcgt ggatttccga aggaatacat cgacgggttc gggctcgatt
    10441 tcgacatgtt cgtctcttct ccggaattgc agcccggtcc tctccattgc ccgttcagcg
    10501 ggttgttctt tctgaaaaca cttcaggacc tgtacggtta ttcggaaatc ccgcgttacc
    10561 tcaccgaggc aatctactgc tcccagtccg aaggatggct cacagagcgg caacaggcgg
    10621 atcactacgt ccgttctcat ctcctcggcc tcgcaagcgg ggttgtgctg ttcgggatga
    10681 ctgccgaact gaacgatccc gggagtgagt acttctacgg atcatacggg ccggtagggt
    10741 tcctgcgccg tgctcctgag ctgaatcccc gtgagagctt ctgcgcgtat gcaaccatga
    10801 cgaggatgct ggatcgcgcc aacttcactg agatggttcc catgccgtcg cccggcacgt
    10861 acgggttgcg cttcacaaaa gctgacggaa gcgacatcta cgcgttctgg acgaccaccg
    10921 gaacgcgcct gctcactttc ggagcggaaa gcggatcggt gcgcactacg gacctgttcg
    10981 gcaaccagac cgtgattcga aacgctcgtg gggtgttctc cctcaatgtc tccccttcgc
    11041 cagtgtacgt cgaaggggaa ggaatccacg ggtttgttct cggaactccc cgccatcgtc
    11101 ccggcccgaa aacaagcgag ccgatcgtgc gtatgggtga cctgccgtca tggcggcacg
    11161 aagccgaaga cgacgcgtgg atggagcggc tcgggcgcgc ggggaactgg gcaatcacgg
    11221 cgccgacaat ccggggcagg tttgagttct cgctgagaaa ggaccgggcg cgcgggcagg
    11281 tcctccgcgt atcactttcc gaaacgccgg gcgctcatcc tctgcacctt cgctacgccg
    11341 tactccggtt ttcccggaag gtgcccgtga acggagcatt ctccgcgctg ggcctctgga
    11401 tacgcggaaa cagctcctcc ggacgtgtcc tttacgagct ggaggacgcc gacgggataa
    11461 tctggcgttc ggtgtacagc gacacctaca tagattttga cggctggcgg tacgtccaga
    11521 cgccgctccc gcttcccgct acggaagacg gatcacggct gcagccgtgt gggctcacac
    11581 cgtgggcaac cgagaccgaa caccgccacc cggcattgcc tgtccgattg accgcgctga
    11641 tcatcgaaag caggactcac gtcgtgcggg caaccagcct cgatccggtc cccgatacga
    11701 cattcgacgt cagcgacgtt gttgtcacca aagaaatggt agacccggaa atacgcttcc
    11761 ggtggtaagg aggcggcgat tcccgctcct cctgcgtttg gagcaaaacg ccgattcgtg
    11821 agggagatgc tctcccgtgt gcacgggcag ttctcatccg gcagaggaca acgctctgcc
    11881 ggtgattccc atcatccttg gcccgacggg ttccggcaaa acggcgctgg tcgtggacct
    11941 cgttcgcgac ggacgcctca ccggtatcga ggttgtctcc gcggattccc ggcaggtgta
    12001 ccgttttctg gatatcggca ctgccaaacc gaccgaggaa gaaagacaca cggtccctca
    12061 taacctcgta gacatccttg acccctccga aacctactcc gccggtcgat ttcgtcggga
    12121 tgcggaggca gtcatcgcgg agatcctgaa acgagggagc accccggtgg ttgtcggggg
    12181 aacggggctc tatctgaagg ccctcgtgga tgggctcagt cccattccaa gggttccggt
    12241 tgacgtggtg gacagactcc gagccgagat cgcgtcagcc ggcccggccg ctctccatga
    12301 gcgactgcag gaactggaca gccgttgcgc agcgacgatc cagccgaccg atacccagcg
    12361 catcatccgc gcgctcgccg tccatgaggc aacagggaag ccactctcgc gctggtggga
    12421 ggagcatccg gagaagccac cttaccgtta tgtctggctg ggaatccggt gggatcgctc
    12481 gcaactgagg gaccgcatcg cccggcgaac tagggcgatg attgcccgtg gccttgaacg
    12541 ggaagtccga tggctgctcg acagggggta cacctgggag atgaacgcga tggccacggt
    12601 cggttaccgg gagtggaagc actgttttgc cggagaaatc acccgtgagg aggtgaccgc
    12661 cgccattgcc attcatacgg cccagtacgc gaaacgccag atgacctggt tcagaacaaa
    12721 cccgcgcatc acatgggttg atggttcgtc gccttccctg aaggatgagg tcgtgcgctg
    12781 gttggatgac cggcacgcgg aggcaaaaag atagccggcg ggcctgtcag gcccaccggc
    12841 cgtgcaatgc gctgcttgtt tcttattcgg ttttgaccgt gatcattttc ggtttcgcgt
    12901 gctctgcctt tggcagagta atctccagca ctccgttgcg atactccgcg ctgactttgt
    12961 ccgggtctac caccgtgggc aggttgaaga cccttttgta ggtgccgtac aatcgctcag
    13021 cacggtgcca ggagaacttg ttggcgtcct ccatcggctt gcgaacaccc gtgatggtga
    13081 gcgtattgcg ctccacgctc aactggatgt cctcacgatt gagcccgggg aggtccgcgt
    13141 agatccgaag attgtcagca tcctcaatta cgtctacccg gggcgcccaa tcgctcaggg
    13201 tctcgcctga aaactcaccg aagcgcgaag acagcgcatc gaacaggcga ttgatttcgt
    13261 tctgaatgct gggcagagtc ggtaccatcg ccatatccaa tccctccttc ctcacacaga
    13321 agtaatcgac tgaatgtaga aggcctgagc catcttcacg gcctgtacat cgcccattgc
    13381 aaccgctgtg ccagtgattc gacggcgccg tggaaagacc gcgcagaagc ctgttggcgc
    13441 ggtacccagg cacaaggctt acatcccgca cgctgtcata atggcgcctt acccgcggtt
    13501 gtggcaaaga tgatgccgtt ttggcagggt gcggcgcaca aggtggcacg gtgaaacgtt
    13561 tttgcgatat ttcatcctgc gcgtacccat tcccacgttg gcgcatcggc cgtaacagat
    13621 cagccgtaca gaggcatgtg aaatggcatt cgaacgacga acacagcttt ccctctggta
    13681 tttcgtcggt gccttcatga ttctgctggc tctgaacctg tatttctcaa aatctcaggt
    13741 cgaaccgctg aattacagcg tgttccggca gtatctgaaa cagggacgta tcgttgaggc
    13801 gactcttacc gcccagcata tcaccggtgc gatgcgaacc ggtacatcaa cctccacgct
    13861 tgcccgtttt gtgacggtgc gcgtgacgga ccctgagctt ctggaccttc tcacccagca
    13921 tggagtcatc ttccgcggac gcgtggaaga cacatgggta accgaaatgt tggcgtggat
    13981 cggtggcatg gcggtgtttt tcgtcatctg gatgttcctc attcggcgca tggggggacc
    14041 ctccagcggg atgatgtcca tcggaaagag caaagccaag atctacgtcg agacggaaac
    14101 caaagtgacc ttcgccgatg tcgcgggaat tgacgaagcg gaagaagaag tccgcgaagt
    14161 ggtggaattc ctgaaagaac cggagcgttt ccggaaactc ggcgggcata ttcccaaggg
    14221 agtcctgctg gtcggtcctc cggggaccgg taaaaccctt ctggcgaggg cagtggccgg
    14281 ggaatccggg gtgccgttct tcagtctgtc cggatccgat ttcgtggaga tgtttgtcgg
    14341 cgttggcgcc gcgcgcgtgc gggatctttt ccagcaggcg aaggagcggg cgccctgcat
    14401 cgttttcatc gacgagcttg atgcgctcgg caaagcgcgg gggatgaatc cgctgggaag
    14461 ccacgacgag cgggaacaga ctctcaacca gctgcttgtc gaaatggatg gattcgatgc
    14521 gaacgtaagc atcatcatca tggcggcgac gaaccggccg gaaatccttg acatggcttt
    14581 gctgcgtccg gggcgtttcg atcgtcagat cgtggtggat cgtccggatc tcaacggtcg
    14641 tctggaaatc ctgaaaatcc atgcgaaacg cgtgaaactc gacccggaag ttgacttacg
    14701 gcaactcgct gcgggaacgc cgggaatggt cggggcggac ctcgccaacg tggtcaatga
    14761 agcagcgctt cttgccgctc ggcgggggca cgacgcgatt tcccagaacg atctccagga
    14821 ggcggtggaa cgcacgctcg tcgggctgga aaagaagagc cgcgtgctga acgaacgtga
    14881 gaggaaaatt gtcgcgtatc acgaggccgg gcacgcgctt gcgggtcttt gcagtccgca
    14941 cgccacgccc gtacacaggg tgacgataat tccccgc
//
LOCUS       DAOTNG010000032        14643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4018_length_14643_cov_4.789485, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000032 DAOTNG010000000
VERSION     DAOTNG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14643
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_14643_cov_4.789485"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2144
                     /locus_tag="P5179_02735"
     CDS             <1..2144
                     /locus_tag="P5179_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRS94167.1"
                     /translation="DDTGFTIARLVREPDKVPVTIVGTIPACTIGETVEVSGEWKSHP
                     SYGEQLRLTSYRTILPSSSEGLKRYLGSGLIKGIGPAFAEKIVRKFGAETLDVITKHP
                     DRLREIEGIGPTRVRQIQAAWREQADIRELMLFLQHHEIPTGVAHRIYRKYGAESVAV
                     IKADPYRLADEISGIGFKTADKIAEHVGIGQDDPRRIEAGIKYVLNRSTEQGHTYLPA
                     DELTQETAQQLSVPVSAILPIMERAARAGEILFEANRVSLPRYVFCERQIAQCLADLI
                     THHPSPRNNEIQASVIARVESRTGVQYAPEQAAAIMASLQEGVLVITGGPGTGKTTTT
                     QGIVSELVAERKRLLLCAPTGRAAKRLAEVTGHEAKTIHRLLGFVPGGHEFQHDERHP
                     LPADVVLVDEASMVDTELFLHLVRALPSGCRLILVGDADQLPSVGPGQVLRDIIASQV
                     VATVALRTVFRQAQASMIVTNAHAINQGVFPQLPNTREGDFFFVYREDPEEIAATIAD
                     LVTRRLPFRYKLNRFTEIQVLTPMYRGETGANALNKLLQEKLNPGGASVPRGTTEFRV
                     GDKVIVTRNNYSKMVFNGDIGRIVGVDAENAKVSVELNLSAADTVRVTYEFDELDELS
                     LAYAISVHRSQGSEFPAVVMPITTQHYMMLQRNVLYTAVTRAKRLLVLVGSKKALAIA
                     IRNGSATGRYTMLAQEIRDAVSAQRAHSPEK"
     gene            2104..2967
                     /gene="pyrF"
                     /locus_tag="P5179_02740"
     CDS             2104..2967
                     /gene="pyrF"
                     /locus_tag="P5179_02740"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRS94168.1"
                     /translation="MPYPHNVPIPRRSNVDFLAKLKAAARTRGSWLCVGLDPIVEKLP
                     ACLPRSSTGVTAFCRRIIESTAPHACAFKPNIAFFEALGRDAFSILEDVISSVPEDIP
                     VILDAKRGDIGSTAHLYARSYFEKLGVDAVTLSPYLGWDAIEPFLGYPGKAGFVLCLT
                     SNPSAAQIQMLPCEGEPLYVRVARLVQSQNPSTGLVVGGTRPEQIGIVREICPESPFL
                     IPGIGAQGGDLELSVRLGSWKTGGGALINASRAVLYASNGEDFAEAAGGVAAGLKEEI
                     ARAGSAFQRSP"
     gene            complement(2952..3332)
                     /locus_tag="P5179_02745"
     CDS             complement(2952..3332)
                     /locus_tag="P5179_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS94169.1"
                     /translation="MLSVLVVDDETGARTMLAMALRGADIHVDMAADGPTALELIGKN
                     PYDWVVSDVKLPGMDGVTLIKQIGRLSPKTRMLLISAVLHEDDVRALPIDGFWRKPFD
                     PFAIQRFIVENANAADSRKAYGLR"
     gene            complement(3543..4922)
                     /locus_tag="P5179_02750"
     CDS             complement(3543..4922)
                     /locus_tag="P5179_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS94170.1"
                     /translation="MATPRVLLVDDERAVVGLLALALKDAGYHADVEHTGTAAVGRLS
                     DEVYDAVITDLRLDDMTGIDILRIACKRYPSPAVLIMTGYGSVDSAVEAMKLGAADYL
                     TKPVYPQELLLTLERALQHRQLLSELDALRQRVGQLQLDDLIGISPAMQTVFRTIQRI
                     ARTDVTVLITGESGTGKELVARAIHNHSSRAAHPFLTVNCSALTETLLESELFGHAKG
                     AFTGAIVAKKGLFEDADGGTLLLDEIGDMELGAQAALLRVLESGEVRRVGETRPVGVD
                     VRIVASTNRDLENAIREGKFREDLYYRLRVVPVDIPPLRKRLDDVLPLAQHFLARYAP
                     KFNRGHVRLAKETGEALLRYTWPGNVRELEHAIERALLLSDGDVLLPEDLPPEVSSPP
                     ASRANTTQLTLAEMERQHILERLDQCSGNRSEAARQLGISRNTLARKLKSYGFSDEET
                     NEASDEAFP"
     gene            complement(4891..6624)
                     /locus_tag="P5179_02755"
     CDS             complement(4891..6624)
                     /locus_tag="P5179_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS94171.1"
                     /translation="MRNHLSADDFKTRIQWHPEDAVIRVDGRRKILVDVDAMHALRET
                     IVNTVGQERARGIYRRFGYGCGKADFEQAAATNPEASPDDLLALGLQAHMFMGHVHAE
                     LTETRLDMAKKDLRAEGVWRHSFETDCGVPLGPSSCWMLQGYASGWASALVGKPVVAL
                     ERQCTGAGAPFCIFELRTPEKPFGPEQVFLDDLGQVNLEDHIGLLEQLAADLRTAQTR
                     LMGSEAKYRALFENAPDMMMLCDPVTGRILDVNRAVVERLGYERDAVLRMTLRDLHPQ
                     KDHATLSKVIGPSITDPVGSSPMQLVGSGKQVCSVEAAFSLVPFAGSTVLQAIYHDVT
                     GFQTAQLALREAEELARIGRMASAVAHEIRNPLSAIVSGIRLLTSSKRSEEERSLIFD
                     TILVESERLDSTLNDFLQFARPRNPRRKPENLEEMLQDLVRIIWSDKDAVGSVVPRIT
                     VSPDLPLVNCDGDQIRQVFWNVILNAIQAMAGQGELAIDVTYENGLVRVSIADTGPGI
                     PPEELERVFEPFHTTKPRGTGLGLPIARRIIQAHGGAIALESQPGAGTRVTFTLTSEG
                     IDGNSQSSARR"
     gene            7098..8534
                     /gene="dnaA"
                     /locus_tag="P5179_02760"
     CDS             7098..8534
                     /gene="dnaA"
                     /locus_tag="P5179_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRS94172.1"
                     /translation="MTCSPAEAWTQCLVALKGKLFSQSYDTWIKPLVCTRFDSELVVL
                     EAPYSFSVSWIREHYLRDIERTIRKLFNISPRVEFELLPGSSDEETRLLSGEPHVQIG
                     PGTSDRHAATSRKSAPRTDKEPAETETLSLVNKDYTFGSFVIGSNNEVAQSAAKTVAR
                     NPGRTPFNPLLIYGGVGLGKTHLLHAVANECALAGNAKNVIYLTSEQFVREFVTSLKE
                     NRTLQFARRYRQADVMLVDDIQFFQGKERMQEEFFHTFNTLHGQGKQIVLSSDRPPEA
                     LGGLQDRLLSRFQWGLVADIQPPDLETKIAICEMKAEATGLHLDDSAAEFIASQVKSN
                     IRELEGIIKRLLFLVQNHDAKLTLDIVESAVRTMRLAAPETVTVKRIQEAVASHYGIS
                     VDLLTGKSRRSDVVSKRQLAMYLCKLLTTESLRAIGAAFGGRDHATVIHACERIEAAL
                     AEQRSLVLELNSISVALGVSGRIRTNVA"
     gene            complement(8597..10990)
                     /locus_tag="P5179_02765"
     CDS             complement(8597..10990)
                     /locus_tag="P5179_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94173.1"
                     /translation="MEPLLLLDIHHSGYFGWGVWVSPDAIEHWTAETVERMMRNPRYK
                     MGLNLGAHTYENSPELARRIRSWMTSFPDRLFVTGGDYAQPTACVRSGESNLRHLLVG
                     WEEIRRQLGVRVDIWTVSEPGNFAQLPQILKDLGYRGALLRIHGPGQLGSPVPKSDSA
                     SVWWTGPDCTKILAVPEYSGDRLQKGDACTYSMWIMTRYRNTRATRGNYTLDDLWNWK
                     IAQERKGISPVVMSKDDDHNNQYTNNNLCMDSGHALAADTENDPRFRWVTAEELFEAV
                     PTPTCSLAADPNLFETRVSSFCDYGFMGNRDWVVDHRAEAELRMGDFSRALAAMLLGD
                     VPGEKEMREAWKLHLGAQNHDVSLKSLSTVMYHLQHEASRIARGVSGRVLRTIAGEIE
                     TGDGFGAVIVWNPLAFQRKEYATVTLPKEMASKAALHLGETPIQWEVVSEEAENVEMG
                     FVADVPSLGYQTYAVRPGAQAETRCAARVEGRTITTPYFSVTFGERGGIDGLVLKGSE
                     QSVVVPGTVGLSGDIAGERVRSGGSLDIEVGQVSTVARESGWLGKFHRFEITYRVVPD
                     VPYITVHVHIWPEFTEGTPGAPGRAGDPGEKVRLVTTLNPDLGPIRCVRQQPLLIWEY
                     DPSLSPVFAALEWVDYQGTDLGLALLNDGAIGQSVVGAAPEVGAILLNGGISDYHGDL
                     ALMPHRSDWQGATVHEAGQCFGSPLNGVFEVSHPGRLPKRFSLASVTPRNVTVSSVFR
                     ERGKTYARVWEHSGEPARVQFSREGVLLQAERVNLRLRKCAGGSNLEPRRIAVFRIG"
     gene            11222..12385
                     /locus_tag="P5179_02770"
     CDS             11222..12385
                     /locus_tag="P5179_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94174.1"
                     /translation="MTSRERVRAAFQHRQPDRTPIFEREIKHPTDNAILGRPAIHPYN
                     WPEFMRVWAREGWRVAMERYADDEFEILRLLEFDMIRLVPNPPSSAPRPVELDTFTFQ
                     QGDSVLRYHPASGVVEDVNARTRDLDRWENDFRANVEKEYVPPSLSDDQLFVWRRIRR
                     HIADAGLDVAIYVSLYAIPVAALPPFALEWFVTAPELLDRYYEKQSQWIIDTARLFRR
                     EGAEIAGLGGDFAGDTGPVISPRVYRERVVPHIQRQSDALHEMGYWTTNTTDGYLWDV
                     LPDFLERSHVDGYGEIDVAAGMDLARLKKEWGERFTFLGNLDIRWVLCEGTLEEARAE
                     MERCIVQGWGNGGHIIMTSNVVHEDVRPELYLAAIDRYREYFGLPPRKNACTI"
     gene            complement(12449..13444)
                     /locus_tag="P5179_02775"
     CDS             complement(12449..13444)
                     /locus_tag="P5179_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS94175.1"
                     /translation="MRVVVLGATGLLGSGITRVLASRGHTVCAVARGNRQVVLPEGVQ
                     FTPTDRRDRAAMEHLVKSFRPDAVVDCVAYTREDGIIDRDVFAGKVAHLIMVSTDFVY
                     KPSFQVLPITEDAPVRAGTAYSEGKVDCEEVLLRAQELKVTVIRPPHIMGPGGKLGSG
                     SIQGRDSSLIDRVRKGVPVVLIDAGIHLLQPLHVDDAGDAVNAILCNTACFGKVYNVC
                     GPVAGLTRRYYEVIAETLGVPLQILSIAGDIWVAARPDQVSFVRHRVYSLERIHTDTG
                     WFPAITMETSVRRTVADILARGEDVPYVPSQAEERLLAALSRHSAEIRGILASLG"
     gene            complement(13456..13896)
                     /locus_tag="P5179_02780"
     CDS             complement(13456..13896)
                     /locus_tag="P5179_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS94176.1"
                     /translation="MVNLKVILHPTDFSPLSGNALRYGVEFARRFGARLIVLHVLEYA
                     YSAISLAEEAVADAENELQKVLSPSQKEGLDVEMLLVRGKPCEEILRLAREREVDLIV
                     MGTHNRGTLEHALLGSTTEKVIRKAPCPVLTIRHPEHEFIQQGT"
     gene            14199..>14643
                     /locus_tag="P5179_02785"
     CDS             14199..>14643
                     /locus_tag="P5179_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94177.1"
                     /translation="MEIDSRLIGVYRSVGGAIQAASAYSMERIPRTDPLTGVRILQLT
                     SYPVLSHTLYYHCPSITPDSKTLVFMTYNRAGRFSTPDAWRVNLDGTEIRPVTNREGL
                     AGFVISPDGKTVYCQQGGTLLAAPMENSPTGNIAYEVASIPDVVIG"
BASE COUNT         3081 a         4382 c         4108 g         3072 t
ORIGIN      
        1 acgatgatac cgggtttacg atcgcacggc tcgtgcgtga gcctgacaag gtgccggtaa
       61 cgatagtggg cactatcccc gcctgcacaa tcggggaaac ggttgaagtc tccggcgaat
      121 ggaaatcaca cccgtcatac ggcgagcagt tgcgcctcac cagctatcgt acaattctgc
      181 cttcgtcttc ggaaggactg aaacgttacc tgggcagtgg cctgatcaag ggaatcgggc
      241 ctgcgtttgc agagaagatc gtacggaaat tcggcgcgga gactctggac gtaatcacga
      301 aacacccgga ccggctccgc gaaatagaag gaatcggccc cacccgcgta agacagattc
      361 aggcggcatg gagagaacag gcagatatcc gcgaactgat gcttttcctg caacatcacg
      421 aaatccccac tggagtcgcg catcgcattt accgcaagta cggcgctgaa tcagtcgcag
      481 tgatcaaggc tgacccgtac cgcctcgcgg acgaaatttc cggtatcggg ttcaagaccg
      541 ctgacaagat cgcggaacac gtcggcatag ggcaggacga tccccgccgc atcgaagccg
      601 gaatcaaata cgtcctcaac cgctccaccg aacaaggcca cacgtacctt ccggcagacg
      661 aattgacgca ggaaacagcg cagcagcttt cggttcccgt ttcggcgatt ttgccgataa
      721 tggagcgcgc ggcgcgagcc ggtgaaatcc tttttgaggc caatcgtgtc tcactcccgc
      781 ggtatgtgtt ctgcgaacgc cagatcgcac agtgccttgc tgatctgatc acgcaccatc
      841 cttcaccccg caacaatgaa attcaggcgt cggtgatagc ccgcgtcgaa tcgcgcaccg
      901 gtgtgcagta tgcgcctgaa caggctgcgg cgatcatggc atcgttgcaa gagggagtcc
      961 tcgtcatcac ggggggaccg ggaaccggca aaaccacaac cacccaaggg atcgtgagtg
     1021 agctggtcgc ggaacgaaag agacttctcc tctgtgcgcc tacgggccgg gccgccaaac
     1081 gactcgccga agtgaccggg cacgaggcaa agacgattca ccgtctcctt ggttttgttc
     1141 ccggcgggca tgaattccag cacgacgagc ggcatccact tcccgcggat gtggttcttg
     1201 tggacgaagc ctcaatggtg gacacggagc tcttcctcca tttggtgcgg gcgcttcctt
     1261 ccggatgcag attgatcctt gtcggcgatg cggaccagtt gccgtcggtg ggaccgggtc
     1321 aggtgctccg cgacatcatc gcttctcagg ttgttgcgac ggtggcgctg cgcacggtgt
     1381 tcaggcaggc acaggcgagt atgatcgtga caaacgccca tgccatcaat cagggtgttt
     1441 tcccgcagct tcccaacaca cgcgaaggcg attttttctt tgtctaccgg gaagatcccg
     1501 aggaaatagc cgctacgatc gccgacctcg tgacacgccg ccttcccttc cggtacaagc
     1561 tcaaccggtt cacggaaatc caggtgctga cacccatgta ccgcggggag accggggcta
     1621 acgcgctgaa caaactgctt caggagaaac ttaaccccgg aggtgcctcc gtgccacgcg
     1681 ggactacgga gttccgcgtg ggtgacaaag ttattgtgac gcgcaacaat tactccaaga
     1741 tggttttcaa tggagatatc ggcaggatcg tgggcgtgga cgccgagaac gccaaggtca
     1801 gcgtcgaact gaacctttcc gcggcggata ccgttcgcgt cacgtacgag tttgacgagc
     1861 tcgacgaact gagcctggcg tacgcgatca gcgttcaccg gagccaggga tctgaattcc
     1921 ccgccgtggt gatgccgatc acgacgcagc actatatgat gctccagcgc aatgtattgt
     1981 acaccgcggt cacgcgggcg aagagactgc ttgtccttgt cggttcgaag aaggcgctcg
     2041 cgattgccat tcggaatggt tcagcgaccg gacgttacac catgctggca caggaaatac
     2101 gggatgccgt atccgcacaa cgtgcccatt ccccggagaa gtaacgtgga ttttctcgcg
     2161 aagttgaaag ctgcagcccg gacacgcggt tcctggctct gcgtcgggct ggatccgatt
     2221 gtggagaagc tcccggcgtg tctaccccgt tcgagcaccg gcgtcacggc tttctgccgg
     2281 cgcatcatcg aatcgaccgc tccgcacgcg tgcgcgttca aaccgaacat cgcctttttc
     2341 gaggctctgg gccgcgatgc cttctctatt ctggaggatg tcatcagcag cgttccggag
     2401 gacataccag taatcctgga tgccaagcgg ggtgatatcg gttcgaccgc gcacctgtac
     2461 gcgagatcct atttcgagaa actcggggta gatgcggtca cgttgagccc ctacctcgga
     2521 tgggacgcaa tcgagccatt cctcgggtac ccgggcaaag ccggtttcgt gctttgtctg
     2581 acttcgaacc cgtccgcggc gcagattcag atgctgccct gcgaggggga accgttgtac
     2641 gtgagggtag cgagactggt gcaatctcag aacccatcca ccgggctggt ggtcggtggc
     2701 acccggcccg agcagatcgg gattgtccgg gagatctgtc cggaatcacc gtttctgata
     2761 cccgggattg gagcacaggg aggagacctg gagctgagcg tcagacttgg ttcgtggaaa
     2821 accggaggcg gcgctctcat caacgcatcg cgggcggttc tctacgcttc gaatggagag
     2881 gattttgccg aagcagcggg cggcgtggct gccggtctca aggaagagat agcacgagcg
     2941 ggttctgcat ttcagcggag tccataggcc ttcctggaat cggcagcatt cgcgttttcc
     3001 acgatgaatc tctggatggc aaacgggtcg aaaggtttgc gccagaagcc gtctatggga
     3061 agcgcccgta cgtcatcctc atgcagtacg gcgcttatga ggagcattcg ggttttcgga
     3121 ctcaggcgac cgatctgttt gatgagcgtc acgccgtcca tgcccggcag ttttacgtcc
     3181 gaaaccaccc agtcgtacgg gtttttcccg atcagttcga gcgcagtcgg gccgtctgcg
     3241 gccatgtcga cgtggatatc cgcgccccgc aatgccattg ccagcatggt gcgcgcaccc
     3301 gtttcgtcgt ccacaaccaa aaccgaaagc attccgcaac ccttctcgat gaaccctgcg
     3361 gccaacgatt actccacatt ccgtgccaga agtgcggtcg ggtgtcgcag cgaaggtgaa
     3421 gagaagcggg aaagacgcca tcaacggggt ttctcttgga gacccggggg ctggcagatc
     3481 ctgattcaac cgggcccgtt gctacatttc gttgcccctt tggttcgttt cttcgcgcca
     3541 ggttatggaa aggcttcgtc gctcgcttcg ttcgtttctt cgtccgaaaa tccgtaactc
     3601 ttcaatttcc gcgcgagcgt attccggctg atgccaagct gtcgcgcggc ttcgcttcga
     3661 ttgccggagc actggtcgag gcgttcgagg atgtgttgcc gttccatttc cgcaagcgtc
     3721 aattgcgtcg tgttggccct gctcgcggga gggctggaca cctcgggcgg aaggtcttcc
     3781 gggaggagca cgtcgccatc agacaacagc agcgcgcgtt cgattgcatg ttccagttcg
     3841 cgcacatttc ccggccatgt gtaccgaagg agagcctcac ctgtttcttt cgcaagccgg
     3901 acgtgtcccc gattgaattt gggggcgtac cgagccagaa aatgctgcgc aagggggagt
     3961 acatcgtcca gtcgttttct gagaggtggg atatccacgg ggacaacccg aagtcggtaa
     4021 tagaggtctt cccggaattt cccttcacga atcgcatttt cgagatcgcg gttcgtggaa
     4081 gccacaatcc gcacatccac tcctaccggc cttgtttcgc ccaccctcct gacctctccc
     4141 gattcaagga cccgcagaag cgctgcctgc gctccaagct ccatgtcccc gatttcgtcg
     4201 aggagaaggg tcccaccgtc ggcatcctca aacaggcctt tttttgcaac gatggccccg
     4261 gtgaatgctc cctttgcgtg gccaaagagt tcactttcga ggagggtctc tgttaacgcg
     4321 ctgcagttca ccgtaagaaa cggatgcgct gcgcgcgagg aatggttgtg gatagcgcga
     4381 gcgaccagct ccttccccgt gccgctttcg ccggtgatga gaactgtcac gtcggtgcgc
     4441 gcaatgcgtt gaattgtgcg gaacaccgtc tgcatcgcgg ggcttatgcc gataaggtcg
     4501 tccaactgca attgaccgac gcgttgacgc aacgcatcca gttcggagag caactggcgg
     4561 tgttggaggg cacgctcaag cgtgagcagc aactcctgcg ggtacacggg tttggtgagg
     4621 tagtccgctg caccgagttt catcgcttcg actgccgaat ccacgctacc gtaccccgtc
     4681 attatcagaa ccgccgggct tgggtagcgt ttgcacgcga ttctgagaat gtcgatcccg
     4741 gtcatatcgt ccagacgcag gtctgtgata actgcgtcgt acacctcgtc tgaaaggcga
     4801 cccaccgctg ccgttcccgt gtgttctaca tcggcgtggt acccggcatc tttcagcgcc
     4861 agcgccagaa gaccaacaac ggcgcgttca tcatctacga gcagaactct gggagttgcc
     4921 atcaatccct tccgaggtca acgtgaatgt cacccgcgta cccgcgcccg gttggctttc
     4981 caaagctatc gctccaccgt gggcctggat aattcgccgt gcgatgggaa ggccaagacc
     5041 ggttccccga ggttttgtag tgtgaaacgg ctcgaacacg cgttcgagtt cctccggagg
     5101 aattccgggg cctgtgtcgg caatcgaaac acggaccaga ccgttctcgt aagtcacatc
     5161 gatggcaagc tctccctggc ctgccattgc ctggattgcg ttcagaatga cgttccagaa
     5221 cacctgtctg atctggtcac cgtcgcaatt cacaagaggc agatcaggag agaccgttat
     5281 tctgggcaca accgaaccga cggcgtcttt gtcgctccag atgattctca cgaggtcctg
     5341 cagcatttcc tcgaggtttt ccggttttct ccgtgggttc cgcgggcgtg cgaactggag
     5401 gaaatcattc aacgtgctgt ccaatcgttc actttcgacg agaatcgtgt cgaaaatcag
     5461 cgaccgttct tcctcagaac gtttggagga cgtgagcagg cggattccgc tcacgatggc
     5521 actcagcgga ttgcgaatct cgtgggcaac ggcagaggcc atacggccaa tgcgcgccag
     5581 ctcttccgcc tcgcgcagag ccagttgggc cgtttgaaaa ccagttacgt cgtggtaaat
     5641 ggcttgaaga acggtggatc ccgcgaatgg aaccagcgaa aacgcagcct ccacggaaca
     5701 aacctgtttt cccgaaccaa ccaattgcat cggcgaagag ccaaccgggt ctgtaatgga
     5761 aggcccgatt accttactca gtgtcgcgtg gtctttttga ggatggagat cgcgcaacgt
     5821 catgcggagc acggcgtcac gttcgtaccc gaggcgttcc accacggcac gattcacgtc
     5881 gagaatacga cccgtcaccg ggtcacacag catcatcatg tcgggcgcgt tctcaaacag
     5941 cgcgcggtat ttcgcctcgg aacccatcag tctcgtctgt gccgtgcgaa gatccgccgc
     6001 cagttgctcc aaaagaccga tgtggtcctc caggttgacc tgcccgaggt cgtccagaaa
     6061 aacctgttcc ggtccgaaag gcttttccgg agttcgcagt tcgaagatgc agaacggcgc
     6121 gcctgctccg gtacactgac gttccagagc aaccacaggt tttcccacaa gggcacttgc
     6181 ccagcctgat gcataccctt gaagcatcca gcacgaagaa ggccccagag gcacgccgca
     6241 gtcggtctcg aaggagtgtc gccacacgcc ttccgcgcga aggtcctttt tcgccatgtc
     6301 cagacgtgtc tctgtcagtt ctgcatgcac gtgacccatg aacatgtgcg cctgcaaccc
     6361 caacgccaga agatcatcgg gagacgcttc cggattggtt gccgcggcct gctcgaagtc
     6421 ggctttcccg catccgtacc caaaacgcct gtagatgcct ctcgcccgtt cctgcccaac
     6481 ggtgttgacg atggtctcgc gcagagcatg catggcatcc acgtcgacga gtatctttcg
     6541 ccgtccgtcc acgcggatga cggcatcctc tggatgccat tgtatgcgag ttttgaagtc
     6601 gtcagcgctg agatgattac gcatcgtgat cgtccctgca gaataccgtt ccgggtcacg
     6661 ctctaaatgt atcggtcgcc tttcaaacaa gacaacgctg acacggggtc acccggtttc
     6721 cgcaggttca aatgacgaac ttgccatttt ggcgcatgca atgcgggtac ggcatgcgat
     6781 caatgcgatt ccaccgattt ccaccacagg tagtgggtgt gaatttttga acaccaagga
     6841 aacccaaaaa aagaaaaacc actgttcaaa aatgcattgt ttccttcggg attgacccct
     6901 tagcttaacc ttacactaac cgagtatctc agcgtgtgat tcagcccgtg ccatgaacgg
     6961 catcagcgcc cttcagaagg gattttgacg ccgagggata cgtatttttc gttgatcaca
     7021 acacggcgaa aaacgcaact tggtcaagaa tttgtttatt aagaacttaa cccttttttg
     7081 ataaagatac gcgcgaaatg acctgctcac cggcagaagc ctggactcag tgtctcgttg
     7141 ctttgaaggg gaagttgttc tcccagagct acgatacctg gatcaaaccc ctcgtgtgca
     7201 cccggttcga ctccgaactt gtcgtgctgg aagcgccgta cagcttctcc gtatcgtgga
     7261 ttcgagagca ttacctcagg gacatcgagc gcacgatacg aaagctgttc aatatcagcc
     7321 cgagagttga attcgaactc ctccccggtt cgtcggacga agagacgcgg ctgctctccg
     7381 gcgaaccgca cgtgcagatc ggacccggca catcggaccg tcacgcggcg acgtccagaa
     7441 aatcagcgcc gcgcacggac aaagagcctg cggaaaccga gacgctctcg ctggtgaata
     7501 aggattacac cttcgggtcc ttcgtcatag gctcaaacaa cgaagttgcg cagagcgctg
     7561 cgaaaacggt cgcgcgcaac ccgggccgaa ctcctttcaa ccccctcctg atatacggtg
     7621 gagtgggact tggtaaaaca caccttctgc acgcagtcgc caatgaatgc gcgctcgcgg
     7681 gcaacgcaaa gaacgtcatt tacctcacga gcgaacaatt tgtgcgcgag tttgtgacgt
     7741 ccttgaaaga gaaccggacc ctccagttcg cacgcaggta ccgtcaggcc gatgtaatgc
     7801 tggtggacga tattcagttc tttcagggca aagaacgcat gcaggaggag tttttccaca
     7861 cattcaacac gcttcacggg caaggcaagc aaatagtcct gtcgtccgat cgtccgcctg
     7921 aagcccttgg aggccttcag gaccgcctgc tttcgcgatt ccaatgggga ttggtggccg
     7981 acatacagcc tcctgatctc gaaacaaaaa tcgcgatctg cgagatgaag gccgaggcga
     8041 cgggcctgca tctcgatgac agcgccgcgg aattcatcgc ctcacaggtg aaaagcaaca
     8101 tccgggagct tgaagggatc atcaaacggt tgcttttcct tgtgcagaac cacgatgcga
     8161 agttgacgct ggacatcgtg gaaagtgcgg ttcgcaccat gcgccttgcg gcgccggaga
     8221 ccgtcaccgt gaagcgcatc caggaagctg tcgcctccca ctacggtatt tctgttgatc
     8281 tcctgaccgg aaaaagccgg cgttcggacg tcgtcagcaa acgccagctt gcgatgtacc
     8341 tgtgcaaatt gctgacgacg gagagcctca gggccatagg ggccgcgttt ggtgggcgcg
     8401 accatgcgac ggtgattcac gcgtgcgaac ggattgaggc ggcgctggca gagcagcgat
     8461 ccctcgttct ggagctgaat tccatctccg tagcgctcgg tgtgagcggc agaatacgaa
     8521 caaatgtcgc atgaacgccg caggtagcga ctgaacgcct tttctttccc accgcacacc
     8581 agatcgccgg tttccgtcag cctatccgga agacggcgat tcttcgcggc tcgaggttgc
     8641 tgcctcccgc gcacttccgc aatctcaggt tcacccgttc ggcttgcaac aaaacccctt
     8701 ctcgcgaaaa ttgaacgcgg gccggctctc ctgaatgctc ccatacgcgg gcgtaggtct
     8761 tcccccgttc ccggaacaca ctgctcactg tcacgtttcg gggcgtaacc gatgcaagac
     8821 tgaatcgttt cggcaaccga cccgggtgag acacctcgaa aacgccgttc agaggactcc
     8881 cgaagcattg gccagcctcg tgcacggtcg cgccttgcca atcactccgg tgcggcatga
     8941 gcgcgagatc accgtggtag tcggagattc cgccgttcag aagaatcgcc ccgacttcag
     9001 gggccgcccc aaccacgctc tggccgatcg caccgtcgtt gagcaacgcc aacccaagat
     9061 cggtgccttg atagtccacc cattcaagcg cggcgaaaac gggagagagc gaggggtcgt
     9121 actcccagat gagcagcggt tgctgcctta cgcatctgat cggccccaaa tccggattca
     9181 gcgttgtgac caaacggacc ttctcgccgg ggtcccctgc ccttccgggt gcgccaggag
     9241 taccttctgt aaactccggc cagatgtgaa cgtgaaccgt tatgtagggg acatccggaa
     9301 caacgcggta ggtgatttcg aaccgatgga atttgcccag ccaccctgat tcccgggcca
     9361 ccgtggagac ctgaccgacc tcgatatcaa ggcttcctcc ggagcgaaca cgttcccccg
     9421 caatgtcacc cgaaagcccg accgtaccgg gaaccaccac cgactgttcg cttcctttca
     9481 gcaccaatcc gtcgatgccg cctcgttcac caaatgtcac cgagaaatac ggagtcgtga
     9541 ttgtccttcc ctcgacgcgc gcggcgcaac gtgtctccgc ctgcgcaccg gggcggacgg
     9601 cgtacgtctg gtagccaagg gatggcacgt ccgcaacgaa ccccatttcg acgttttccg
     9661 cttcctcgga caccacctcc cactgaattg gcgtctcacc aaggtgcagc gctgccttgc
     9721 ttgccatctc tttcggcaga gtaacggtgg cgtactcttt ccgctggaac gccagtggat
     9781 tccatacgat tacggcgcca aacccgtccc ccgtctcaat ctcgcccgca atcgttctca
     9841 aaacccgacc agacacacct cgtgcgatcc gcgaggcctc gtgttggagg tggtacatca
     9901 ctgtgctcag acttttgagg gatacgtcat ggttctgcgc accgaggtga agcttccacg
     9961 cctcacgcat ttctttctca ccaggcacat cacctagaag catggccgca agcgcccggg
    10021 aaaagtcacc catccgaagc tctgcttctg ccctgtggtc gaccacccag tcgcggtttc
    10081 ccatgaaccc gtaatcgcag aacgaactca cgcgcgtttc gaaaaggttc ggatcagcgg
    10141 ccaacgaaca cgtgggagtc ggaacggctt cgaacagctc ctccgccgtg acccaccgaa
    10201 aacgagggtc gttttccgtg tccgcggcga gcgcgtggcc ggaatccata caaagattgt
    10261 tgttggtgta ctggttgttg tggtcgtcgt ccttcgacat gacaaccggc gagattccct
    10321 ttcgttcctg cgcgatcttc cagttccaca ggtcgtccag cgtataattg cctcgtgtgg
    10381 ccctcgtatt cctgtaccgc gtcatgatcc acatggagta cgtgcaagcg tcgccttttt
    10441 ggagccggtc gcctgagtat tcgggaactg ccagaatctt cgtgcagtcg ggcccagtcc
    10501 accataccga agcggaatct gacttcggca ctgggctgcc caattgaccg ggcccgtgga
    10561 tacgcagcaa cgcaccgcgg tatccaaggt cctttaatat ctgaggtagc tgcgcgaagt
    10621 tccccggttc gctgactgtc cagatatcta ctctgactcc aagctggcgg cgaatttcct
    10681 cccaaccgac aagaaggtga cgcagattcg attctcccga acgaacgcac gcggttggtt
    10741 gcgcgtagtc gccgccggtg acgaaaagcc ggtcgggaaa tgacgtcatc caactgcgaa
    10801 tcctccgtgc caattccggg ctgttctcat acgtgtgcgc accgagattc agccccatct
    10861 tgtaacgggg gttccgcatc attctttcca ctgtttcggc tgtccaatgt tcaatcgcgt
    10921 cgggtgaaac ccatacaccc cagccgaaat acccggaatg gtgtatgtcc agaagcagga
    10981 gcggttccat gttctccgaa tgggatgtct ttttcgacac gggagatcct ccgattgcag
    11041 taatcctgtg gggtgtgaac acagagcaag ccgcgaacgc agattgagca aaaaacgagt
    11101 ccggcgagac ggccttgacc gtttcaaaat cgcccgtaac ttactatgga caaaacccac
    11161 cggtacgtcg tgagccagac ctcgcgacgc taggcttgct tcacgacaca aggtctccct
    11221 tatgacttca cgagagcgtg ttcgcgcagc gttccagcac agacaaccgg acagaactcc
    11281 catcttcgaa cgcgaaatca aacacccgac agataacgcg atcctgggaa ggcctgccat
    11341 acacccgtac aactggccgg agttcatgcg cgtctgggcg cgcgaggggt ggcgcgttgc
    11401 aatggagcgt tatgcggacg atgagttcga gatactccgg ctgcttgaat tcgacatgat
    11461 ccgcctcgtc ccgaatcctc cctcttctgc gccgaggccg gtggagctcg acactttcac
    11521 cttccaacag ggagattcgg ttctccgcta ccacccggct tccggcgtcg tcgaggacgt
    11581 caacgcgagg acacgtgatc tggatcgctg ggagaacgat ttccgtgcga acgtggagaa
    11641 ggaatacgtg cctccgagtc tgagcgacga ccaattgttc gtctggcggc gaatccgcag
    11701 gcacatcgct gacgccgggc tggatgtcgc gatctacgtc tcgctctacg ccatccctgt
    11761 agccgcgctg ccgccctttg cgctggaatg gttcgtaacc gcaccggaac tcttggaccg
    11821 ctactatgag aaacagtcgc agtggatcat cgacacggca cgccttttcc gaagggaagg
    11881 cgccgaaata gcgggtctcg gcggcgattt cgcgggcgac acgggtcccg taatctcgcc
    11941 gcgtgtgtac cgggaacggg tagtcccgca tatccagcgg caatctgatg ccttgcacga
    12001 aatggggtac tggaccacca acaccaccga cggatacctg tgggacgtcc tgcccgattt
    12061 tctcgaaaga tcacatgtgg atggctacgg cgaaatcgac gtggcggccg gaatggacct
    12121 tgcccgcctc aaaaaggaat ggggggagcg ttttacgttt ctcggcaatc tggatattcg
    12181 gtgggttctc tgtgagggga cgctcgagga agcacgagcg gagatggagc ggtgcattgt
    12241 ccagggatgg ggaaacggcg gccacatcat catgacgagc aatgtggtgc atgaggacgt
    12301 tcgcccggag ctctacctcg ccgcaatcga ccggtatcgc gagtatttcg gtttgccacc
    12361 gagaaaaaac gcgtgcacca tctgagttca gcgaccgctg ttgtctggac acattctcag
    12421 ggcgcggtac gaagcctcgg gtatccgtct atcccaacga ggcgagtatc cctctgatct
    12481 ctgccgagtg ccttgaaagc gccgcaagga gccgctcttc agcctgcgat ggtacgtacg
    12541 gcacgtcttc cccacgcgca aggatgtctg ccaccgtccg acgcacggag gtctccatag
    12601 taatcgccgg gaaccaaccc gtgtccgtgt gtatcctttc cagactgtac acccggtgcc
    12661 gaacgaagct gacctgatca ggtcgcgcgg caacccagat atcaccggcg attgacagaa
    12721 tctggagggg aactccgagc gtctccgcaa tgacttcgta gtaccgccgg gtcaagcctg
    12781 caaccgggcc acacacgttg tagactttcc caaaacaggc agtgttgcag aggatcgcat
    12841 tcaccgcgtc tcccgcgtcg tccacgtgaa gaggttggag caggtggatg cctgcgtcaa
    12901 taaggacgac gggcacacct ttccgcacgc ggtctatgag tgaagaatct ctgccctgaa
    12961 tcgagccgct gccgagtttt ccacctggtc ccattatgtg aggagggcgt atcactgtga
    13021 ccttgagttc ttgcgcgcgt aagagcacct cctcgcaatc cacctttccc tcgctgtacg
    13081 ccgttccagc gcgaaccggt gcgtcttcag tgattggaag aacctggaag gaaggcttgt
    13141 acacgaagtc cgtactcacc atgatcaggt gcgcgacttt tcccgcgaac acatcacggt
    13201 caatgatccc gtcctctcgc gtgtatgcta cgcaatcaac gacggcatcc ggtcggaagg
    13261 atttgacgag gtgttccatc gcggctcgat cgcgcctgtc ggtgggagtg aattgcactc
    13321 cctccggtag cacgacttgc ctgttgcccc gcgcaaccgc gcaaaccgtg tgtccgcgcg
    13381 acgcaagaac cctcgtgatt cctgagccca gaagcccggt tgccccaagt acaacgacgc
    13441 gcatggtgtt gcccttcaag taccctgttg gatgaactcg tgctccgggt gacggatcgt
    13501 cagaaccggg cagggagctt ttctgatgac tttctccgtc gtgcttccca gcaacgcatg
    13561 ttcaagagta ccccgattgt gggtgcccat gactatgagg tcgacctccc gttcgcgcgc
    13621 caggcggagg atttcttcgc aaggctttcc gcggacaagc aacatctcaa cgtccagacc
    13681 ctccttctga gacggggaga gcaccttctg caactcattc tccgcgtctg ccacggcctc
    13741 ttcggcgagc gaaatcgccg aatatgcata ctccagaacg tgcaacacga tcagtctggc
    13801 cccgaacctc ctggcgaatt cgactccgta gcgaagcgcg tttccggata atgggctgaa
    13861 atcggtcggg tggagtatca ccttcaggtt caccatggca cgcctccctt cccgttgatg
    13921 aatctgttgt acatcacact gtgggcctac cggtgttttg cagcggcaca cgcggttgct
    13981 caagcattat tcgcgggtgt gccgagttgt acaccgaaat atcggttcgt ggcggccccc
    14041 gtcaatctct ccctctctcc gccagttttg acgaagaaag aaaaatccgt attctatcaa
    14101 tcgttcattc tgtttcgttc tgatgcaaat gggacgggct ctccgtcctg ttctttccgc
    14161 ccttcgccgc atccgcacac cccagaaaag ggaaagcaat ggaaatcgac tcgcgattga
    14221 taggcgtcta ccgttccgtg ggtggcgcaa tccaggccgc cagcgcatac agcatggaac
    14281 ggattccgcg aacagaccct cttacaggcg ttcggatact tcaactgacg tcctatcccg
    14341 tcctcagcca caccctttac taccattgcc cttccataac gccagactcg aagacactcg
    14401 tcttcatgac gtacaatcga gcgggaagat tttccacgcc tgacgcgtgg agggtgaacc
    14461 tcgatggaac ggaaatccgt ccggtgacga atcgggaagg tcttgcagga tttgtgatct
    14521 cgccggatgg aaagacggtc tattgccagc agggaggaac tcttcttgcc gctccgatgg
    14581 agaactcccc gactggcaac attgcgtatg aagttgccag catcccggac gtggttatcg
    14641 gcg
//
LOCUS       DAOTNG010000033        14501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4066_length_14501_cov_5.330957, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000033 DAOTNG010000000
VERSION     DAOTNG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14501
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_14501_cov_5.330957"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="P5179_02790"
     CDS             complement(<1..832)
                     /locus_tag="P5179_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS94178.1"
                     /translation="METLFTHAAVWLFLAVISAVLANHLKVSIALMEICMGVAAAAII
                     THFWGANSFPNNEEWLKFLASSGAVLLTFLAGAELEPEVMKTKLKEVSIVGFVGFLAP
                     FVGCALVAHYGLGWDLRASLLAGIALSTTSMAVVYAVMLETGFNRTEFGKGILGACFV
                     NDLGTVLALGLIFAPFTYRTFVFIGGCLVVFFILPYATSFLTRHYGHRTAAIRTKWIV
                     FVLFALGALALWSGSEAVLPAYIAGMVLAEFTSDDAHFMRRLRTLTVGFLTPFYFLRA
                     G"
     regulatory      complement(912..981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1370..5380
                     /gene="porU"
                     /locus_tag="P5179_02795"
     CDS             1370..5380
                     /gene="porU"
                     /locus_tag="P5179_02795"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRS94179.1"
                     /translation="MLVGAALSVCAAGSAPARWELIQADASGVVAEWSSPVVRMDTVV
                     VAGQRTLQPVLEDCSADGEPGSPAIPKAGSLIAVPDGAQASASAVAGEWETRPGLLSP
                     VPSHELREDGLGYRRVFEIRPEAYSAQDTGTDLVSLEPAGEAYGAHLVRIVVHPVRAN
                     LADKSLAITRSVRVRVQFTSPETGSLRDITPLPSVWEGAVLNAAAYNALQRTSRSAAD
                     RVARVAETSPFASGVWLRVVVDADAMYKITAAALAEADSRFRSAPAERLALYAGSGRE
                     LPLDPQKPRQTALRPVRPIVVDANGDGVFNGTDYLLFYGRSPSGWDLDYQTLDPVYAL
                     NHYTYENVYWLTISPATAIRAEVRNGAVSDPSLPVIERFPFRFHEEPEVTNLNEEGDT
                     DGPYSGVDWQWDQLAPQASRVLQVALLDVAGDTVGVRVGQLRQFSAYGSLQVKVNGVV
                     EPLVEQRQTSTYAWCTGYAPVITPAAQVSLPVEIRNASTGYPIILDYYEIAYWRRFVV
                     RGSSFAFVLPPLRNTIPSTRVVVPLSGVDRTLHRLFEVSDDSGLVEYALPPPDDQGIT
                     RVELRQSGLIERKYLLVAESDWRTPLRMEPKTGATNLRGRTAGVDYVVITHPDFQAEA
                     SRLADLRAQRNGFRTAVVTTRDICDEFSFGMFDPAALRDFLRHAFMNWRDGAADQGLR
                     YTVLLGDGQFDYRNLTRFTTSTANPQPGNWVPPYQEGPVTTDDWFVVFDNTGIASVKI
                     GRLCVQTKEEARAVVGKIIAYERDPERGAWQNRAIMIADDEYDLDRGINSEDYVDDSE
                     RMAGCLRAETVTEKIYSHERQLNQQMQKPSVNRMITDAWSRGAALINYVGHGNPVLWA
                     HERIFTLDGDLPNIANGKRLPVLTALTCSAAHFDAPSTQSMAEVLVNMANGGAVATVA
                     ATRLSYNTENVGFNEIFLSRLYGGAEGRTPRIGDAFWYAKALAAQNPSWLYRQNARKY
                     VLIGDPALTVALPELVVQVQMMGDSLRALAASTLRGSVLVPGATDTLRSFSGSALVNV
                     FDSATPMSYQAAGGNTILYTKPGTSIFRGVVPVQNGVFATELILPIEVSYGGTQAKAV
                     AQVWNDQIDGAGSIGGLGICSRPAASVRDTTGPSIVFTRVDDLDAAIPLRDGAEIPRG
                     EPVRVWLSDPLGVNVGGGIGHRLVAYLNGNRSAPVDLTEDFVHWGSATTGWADIPLSG
                     DLTAQQVVVEAWDNGNNFAADSVFLRVGRGKDIAVSNVIAYPNPMARDGAFTFVLDGV
                     GASTDADATVRVFTVAGRLVDTVTKLGVTDGPVVIPWTPAYQLANGVYLYQISIRRRS
                     DGRTAKALERLAVVGP"
     gene            5402..6568
                     /locus_tag="P5179_02800"
     CDS             5402..6568
                     /locus_tag="P5179_02800"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94180.1"
                     /translation="MKVARFSHFTALVIAGASAAVAFLPSVSRANESQAAVLALLFRP
                     GARELALGGAGVAGARGSAASYYNPALLSWQAIAEGQRYPRAVGTTYYKILQNFGLND
                     MYYMYFPTMFSVKDWGQFSVNVTYLNLGEQQRTSEGGENLGTFNTYTLAVGLSYASKI
                     GVHTSAGITAKWFYDHLADAGTGFEKGDPTGTGFAVDAGIAYRPSDRLYFGAALRNYG
                     PNVQYIDAKQASPTPVNFTVGTSWKAFDTQYNDVTIMADLYKPLVQDYRKSWYLSILR
                     SWADESVYKVEEIPDGQGGVTRVEHRNTLREETRQVDVLTGVEYTYADYVAIRGGYFR
                     DWDGQRNWMTFGGGLRLPIASTALLVDFAYVHSLNGKGSDPNDGQQVYSIGFTF"
     gene            6700..9423
                     /locus_tag="P5179_02805"
     CDS             6700..9423
                     /locus_tag="P5179_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94181.1"
                     /translation="MHHRGLLFAFFALSSFLAAASARAVETIHLLALRVEFPKEDPDD
                     FTTGGNGLFDMRSGAEALATAGEAGFRYPFDLPPHDGRFLENHLTALRNYLETASQGR
                     TTITWEVFPPHPLPAYKAPQTLASYGSGGSVAEQTRNWVRFLKDALAVSQNDVGSLGR
                     FQSFLVFHASVSLQGTLSAELPALVLTSQEIASSGIPIPPEVQTAWLIPQQIQQPGGV
                     IGLNGAFAKTFLASRGLPVLSNTRTGGAAVGAWTLMDVGSDNFIQRPKTGSSSDTIRV
                     LGFVPCLPSAWEQMRLGWLAPETVRSDTTLLLAGLAAETSLPRALKVPITEDEYLLME
                     LRHSAVETDRYHPAIEYSDGDTGGVWLKPENGLYDAYVPGSGVIVYHVNEARIRQWEP
                     TNSINAQPDRPGIMVVEADGYRDIGVTNLLGHPRANEGIGSRNDPFPVTGERILYPDG
                     EPAPGHPAVITANGSPGGVAISFSPAGTARDTVAVTVRWRTADTPARKGRFIGSAVVG
                     GVAVRRVPLDGVERNLIAVAGSDWNLWAFTDSLEPVGNANGLIGRFWGDVTRKPVIEA
                     DGSVLVVGLTTNTRYRYANGAWDGGTPVVQTPPVPSTMTCHLLRDVRKSDITVTGSGT
                     ITADSYARFGYLPEDPPPPRVVWSATTDSLIAPLSLGDVDRDGFPDILAVSRRSVDVF
                     SRSGTRTITYTLGRADSTEEFAGSALSTGAGNTIVAGKKGLSVFSGLMRRHPAATIPL
                     PAAATGSPALYAVRDEGIHAVVGTADGWLLDIPVSQGDSVIWGQMWGDGASSFALDET
                     GLVMPAAPAEDLMPGDRVFFYPSPVGKEEGMLRFFLSAQADVSVRIFTSMGEQVWETR
                     VPAGETIANAENEIRWPGGTRFATGLYVARITARSADGRQGGATIPVGIVR"
     gene            9432..12551
                     /locus_tag="P5179_02810"
     CDS             9432..12551
                     /locus_tag="P5179_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94182.1"
                     /translation="MRKGVLYSASKRHVCGMCLVVLLFLAAPSICATPIAEFDPPVSA
                     LSWKRFETEHFRITYTSGLERVASAVAMAAEQAYEPVTRLYGYRPSGKTEIIVRDHGD
                     FANGFAAYYDNRMEIWASALDYEFRGTHNWICDVTTHEFTHLVQLGASLKTSPAIPQV
                     YFQWFRLEPEARTDVAEGLPNTLVSYAVPTVSIPMWFAEGVAQFQAQGARHDRWDAHR
                     DMIVRVAVLADSQLSYRQMEGFYDPDGREAEMVYNHGYSFVRWLAATYGDSSLRALSR
                     DMRAWHRWDFGSALKQLTGKPGPRVYEEWLTQLRAGYEAFVAERKPQPAGTLLERPAS
                     RPGAESGDRPPVITPGGVPAAYAPPPAHSARASYYHAAPVWSPDGALLAYVSTDGQDY
                     RLGSVWVRKPGTGELTVAPGSFRASSTVSWFPDGRTVVFSRAQRDDRNEWRYNDLVKA
                     DLQTGTVTKLTDQWRASYPAVSPDGATVAFVRNARGSTNLMLVNADGSNVRPLTRWDD
                     GTQIFSPKWSPDGTRLAVSLARAGSRHIVVYRVVRASGGEVLETERVIASSDDDRDPA
                     FSRDGTRVVFSSATDGVFNLYELDLERGSCARLTNVLGGAFHPDVAPDGRIAYSSYES
                     AGYVIRIIPADAPRYPVSADIFANRIPEQGTFPDSMPEGSSVSDIDRRIPHLQRPSIL
                     PRFGRYGDQWRVGAYAFSSDIWDDVLLMGGVWAAPENRDYDAFVTAEVDHMTPVPVLF
                     DVVRTVRHTKEDTTFAGRLRLDGITYGLNAVSLTLKPRVFSREIDLHSTYQRFDAAID
                     QTLLLNGTRTYVGYNYTYYNGFAAGASVSSSAIRPFTTADIAPQGMKWSLRYDRWWNW
                     YFEDFDAGTGLLNEVYTPYHYNQVSCDAAWYLPTPWREEHTVGVEGRVMRIDAAVDSF
                     FYEGIGGIIGLRGYTYYQLQGKQTAWGRLLYRFPLLTSIDRKLGPVYFDKIYGTVFAE
                     AGRVWRDSYPSSWTPGLKRDVGAEIRADLFSFYGYPSRFSFSAARALDAAPHSDRTKF
                     YLTLLFGYL"
     gene            12882..>14501
                     /locus_tag="P5179_02815"
     CDS             12882..>14501
                     /locus_tag="P5179_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRS94183.1"
                     /translation="MTEHGNGPESGLEIDPSLLDADAVIRALHTDPEHGLSSAEAAAR
                     LARYGKNELVGAPPVPVWRKLLAQFEDPLIYLLLAAVAISAVAWFMEGAHGWPVDSAV
                     ILAIVVANGVLGYVQEAKAENAVAALASMAAATSAVVRNGQVERIPSSDIVPGDVLVL
                     SEGDNIGADGRLFKTANLRVAEAPLTGESEPVQKETSALQEPSNLGDRRNMVFKGTAV
                     TQGVGRAVVTATGMNTEVGKIARMLETTEDEATPLQKEIVRLGKMLGIAVVLIAAVVV
                     TTTFVVFRVDTMDEAVSLLLLGVSLAVAAVPEGLPAVLSVVLAIGVQRMAARNAIVKK
                     LSSAETLGSASVICSDKTGTLTKSEMTVTKIVTASGEVALTGVGYDPSGRAEVAGQEI
                     GDSALRTEAQCVLAGGSLAGNASLRQRDGVWEIQGDPTEAAFLVAAQKLGGTQQIMER
                     YERLGEIPFTSERKRMSVLLRERNTGRLTLFSKGAPDVLLERCTHILVGNEVAPLDDA
                     RRAASVENIEALSAQAFRTIAVAYRNLEGEGV"
BASE COUNT         2812 a         4245 c         4590 g         2854 t
ORIGIN      
        1 aaccggcgcg caggaagtaa aacggtgtca gaaacccgac cgtgagggta cggagccgcc
       61 gcatgaaatg ggcatcgtcg gaggtgaact cggcaaggac catgccggcg atatacgccg
      121 gaagcaccgc ttcactgccg gaccacaggg cgagggcacc gagggcgaag agaacgaaca
      181 caatccattt ggtgcggatt gccgccgttc tgtggccgta gtgccgcgtc agaaaggacg
      241 ttgcgtacgg caggatgaaa aaaaccacaa ggcacccgcc gatgaacaca aacgttctgt
      301 aggtgaaagg ggcgaagatc aggccaaggg caagcaccgt tccgagatcg ttgacgaagc
      361 aggcgcccag gattcccttc ccgaactccg tcctgttgaa tccggtttcc agcatgactg
      421 catagactac cgccatcgag gtagtggaga gcgcgattcc tgccagcagg ctggcccgca
      481 gatcccaccc gagtccgtaa tgggcgacca gcgcgcaacc gacgaagggc gccagaaaac
      541 cgacaaatcc gactatagat acttccttga gcttggtctt catcacctcc ggttcgagtt
      601 ctgcgcccgc cagaaacgtg agaagcaccg cgccggagga cgcgaggaac ttgagccatt
      661 cttcgttgtt cggaaacgag ttggcgcccc agaagtgcgt gattatggcc gcggcagcca
      721 ctcccataca gatttccatc agcgcgatgg atactttcag gtggtttgcc agcacggcag
      781 agatcaccgc caagaaaagc cagacggcgg cgtgggtgaa cagggtttcc atgagtcgac
      841 ctctcagagg atggcgatgg acacaaaaac cgccagaacg cgggaaacaa aaaactcccg
      901 ccggccagta ctcggctgca ggagtcatca gtccaccgtt cgcggacggt tcaatcgggg
      961 aactccatcc ccgtagtaaa tgtacgcagg ccgggagccg cgggcaatga tttctcacaa
     1021 ttggcgcgcg tcgtgcagac cttgacagtt tcgccggcac gcgatacact ttaattgcac
     1081 gcaggcgcga atcagtgcat tcaagatgcc acacagaagc gacgggagaa tccgtatcgc
     1141 acgattgtga tgagagttac gacgagtggt tccgatgaaa gccccgtccg ggcacaagcg
     1201 gaaacgcatt gaaaaagctg ttcgtcgcgc ggtttcacaa tcgtcgggtt cctccgcctt
     1261 gcaggtacta tgagccgagc tggttcaggg cggaacctct gaacgtctcg gctcttggtg
     1321 ttttatgagt cttccccgcg gtttcctgat gccttccccg ggacgggcaa tgctggttgg
     1381 agcagcgttg tcggtctgcg cggccgggtc ggccccggcg cgctgggagt tgattcaggc
     1441 ggatgcgtca ggcgtggttg cggagtggtc gtcgcccgtg gtgcggatgg acacagtggt
     1501 tgtggcgggg cagcgtacgc tccagccggt gcttgaggat tgctcggcgg acggcgaacc
     1561 cggctcgcca gccattccga aggcaggttc gctgatcgcg gttcccgatg gggcacaggc
     1621 gtcggcctct gctgtggcgg gcgaatggga aacgcggccc gggctgctca gtcccgtgcc
     1681 gtcacacgag ctccgcgagg acgggctggg gtaccgccgg gtgttcgaga tccgcccgga
     1741 ggcgtattcc gcgcaggata cggggacaga cctcgtatcg cttgaacctg ccggcgaagc
     1801 ctacggcgcg catctggtgc gtatcgttgt gcatccggtc cgggcgaacc ttgccgacaa
     1861 aagccttgca atcacccgtt cggtgcgcgt tcgggtgcag ttcacatcgc ccgaaaccgg
     1921 gagcctgcgg gatatcactc cgttgccttc cgtctgggaa ggagccgtac tgaacgccgc
     1981 agcgtacaat gcgctccaac ggacaagccg cagcgcggcc gaccgtgtcg cgagggttgc
     2041 ggaaaccagc ccgtttgcct ccggggtgtg gttgcgggtt gtcgttgatg cggacgcgat
     2101 gtacaagatc accgcggcgg cacttgctga agcggacagc cgcttccgga gtgcgcccgc
     2161 ggaaaggctg gcactgtatg ccgggagcgg gcgggaactc cccttggacc cgcagaaacc
     2221 tcgtcagacc gccctccgac cggttcgccc gatcgtggtg gatgcgaatg gcgacggggt
     2281 gttcaacgga accgattatc tgctgttcta cggccgcagc ccgtcaggat gggatctcga
     2341 ttaccagaca ctggatccgg tgtatgcgct caaccactac acgtacgaaa acgtctactg
     2401 gctcaccata tctccggcaa ccgcgatacg tgcggaggtt cgcaacggag cagtgagcga
     2461 cccgtcgctt ccggtaattg aacgtttccc gtttcgattt cacgaagagc ctgaggtaac
     2521 gaatctcaac gaggaaggtg acactgacgg gccgtattcc ggtgttgact ggcaatggga
     2581 tcagcttgcg ccgcaggctt cgcgcgtcct gcaggtggcg ttactggacg ttgccggtga
     2641 cacggtcggg gtccgcgtgg ggcagttacg gcagttttcg gcgtacgggt cgttgcaggt
     2701 gaaggtcaac ggcgtcgtcg agccccttgt tgagcaacga cagacttcca cgtatgcgtg
     2761 gtgcaccggg tatgcgccgg tgatcacccc ggccgcgcag gtcagccttc ctgtggaaat
     2821 ccgtaatgcg agcaccgggt atccgatcat tctggattac tacgaaatcg cctactggcg
     2881 caggttcgtg gtgcggggga gcagtttcgc cttcgtgttg ccgccgctgc gcaacacaat
     2941 cccatcaacg cgagtcgtgg tgcctctttc aggcgtcgac cggacactgc atcgtctttt
     3001 tgaagtgagt gacgacagcg gccttgtgga gtacgcgctt ccgccgccgg acgatcaggg
     3061 gattacgcgg gtggaactca ggcaatcagg gctgatcgag cgcaaatacc ttctggttgc
     3121 cgaatcggac tggagaactc cgctccggat ggaaccgaaa accggcgcga ccaatctgcg
     3181 gggtcgtacc gccggggtgg actacgtcgt cataacccac cccgacttcc aggcggaggc
     3241 atcccggctg gccgatctgc gggcgcagag gaatggattc cgcacggcgg tcgtgacgac
     3301 gcgggacatt tgcgatgagt tctcgttcgg aatgtttgat cctgcagcgc tgcgtgattt
     3361 cctgcgccat gcgttcatga actggcgaga tggcgccgca gaccaggggt tgcggtacac
     3421 agtgctgctt ggtgacgggc agtttgacta ccgcaatctg acgcggttca ccacctccac
     3481 ggcgaacccg cagccgggaa actgggtccc gccctaccag gaggggccgg tcacaacgga
     3541 cgattggttc gtggtgtttg acaacacggg gattgcatcc gtcaagatag ggaggctctg
     3601 cgttcagacg aaggaggagg cgcgggccgt tgtcggaaag attatcgcgt acgagcgtga
     3661 cccggagcgc ggtgcgtggc agaatcgcgc catcatgatt gccgatgatg agtacgatct
     3721 cgaccgggga atcaactccg aagactacgt ggacgatagc gaacgaatgg ccgggtgcct
     3781 gcgcgcggaa accgtgacgg agaagatcta ctcgcacgaa cggcaactga accagcagat
     3841 gcagaagcca tccgtcaaca ggatgatcac cgacgcctgg agcagaggtg ccgcgttgat
     3901 caactatgtt ggccacggaa acccggtcct ttgggcgcac gagcgcattt tcacgctcga
     3961 cggggacctt ccgaacattg ccaacgggaa gcgtctgccg gttctcacgg cgctgacgtg
     4021 cagcgcggcg catttcgatg cgccctcgac gcagagcatg gcggaagtgc tggtaaacat
     4081 ggcgaatggc ggtgcagtag ccacggtggc ggccacgcgt ctttcctaca acaccgaaaa
     4141 cgttgggttc aacgagattt tcctctcgcg gctgtacggt ggcgcggaag gcaggacgcc
     4201 tcgaatcggg gatgcattct ggtacgccaa ggcgctggcc gcgcagaacc cgagctggct
     4261 ttaccgccag aacgcccgca aatacgtact gatcggtgat cctgcgctta cggtcgcact
     4321 tccggagctg gtggtacagg tgcagatgat gggcgattcg ctccgcgcgc ttgcggcctc
     4381 cacgcttcgc gggtcggtgc tggtgccggg agcgacggac acgctgcgaa gcttctccgg
     4441 atcggcgctg gtgaatgtgt tcgacagtgc aacccctatg agctaccagg ccgccggtgg
     4501 aaacacgatc ctctacacaa aaccgggaac gtcgatcttc cgtggagtgg tacctgtgca
     4561 gaacggtgta tttgccaccg aactcattct gccgatcgaa gtgagttacg gcggcacaca
     4621 ggcaaaagct gtggcgcagg tatggaatga ccagattgac ggtgcgggaa gcatcggagg
     4681 actgggaatt tgcagtcgcc cggccgcatc ggtgcgtgat acaacgggac cgagcatcgt
     4741 gttcacgcgt gtagacgatc tggacgcggc gattcctctc agggacggtg ccgagattcc
     4801 gaggggagag ccggtccgcg tatggctttc agatccgctc ggggtcaacg tgggcggagg
     4861 tatcggccat cgtcttgtgg cgtatctcaa cggcaaccgc agcgctccgg tggatctgac
     4921 ggaagacttc gtgcactggg gcagcgcgac caccggctgg gccgacattc cgctttccgg
     4981 cgatctcacc gcccagcagg ttgtggtgga ggcatgggac aacgggaaca attttgccgc
     5041 tgactccgta ttccttcggg tgggtcgagg caaggatatt gctgtgagta atgttatcgc
     5101 gtaccccaac ccgatggcgc gggacggggc gttcactttt gtgctggacg gcgtgggcgc
     5161 gtctaccgac gctgacgcga cggtgcgcgt gttcacggtt gccgggcggc tggtggatac
     5221 ggtgacgaag cttggggtta cggacggacc tgtggtgata ccgtggaccc cggcgtatca
     5281 gttggcgaac ggagtgtacc tctatcagat ttcaatacga cgtcgtagtg acgggcggac
     5341 cgccaaggct ttggagagac tggccgttgt gggtccgtaa ggtttcagga ggaagcgcac
     5401 gatgaaggtt gcacgattct cacatttcac ggctctggtt atcgcaggtg cctctgccgc
     5461 cgtcgcgttt ttgccgtcgg tttcgagggc gaatgaaagc caggccgcgg tgctggcgct
     5521 tctcttccgg ccgggggcac gcgaactcgc gctgggcggc gccggagttg ccggggcgcg
     5581 cgggtcggcg gccagctatt acaacccggc gctgctttcg tggcaggcaa tcgccgaagg
     5641 gcagcgatat ccgcgcgccg tcggcacgac gtattacaag attctccaga acttcggtct
     5701 gaacgacatg tattacatgt atttcccgac gatgttcagt gtgaaggact ggggacagtt
     5761 cagtgtgaac gtcacttacc tcaacctggg tgaacagcag cgcacaagcg aaggcgggga
     5821 aaacctcggc accttcaaca cctacacact ggccgtcggc ctgagctatg cgtcgaagat
     5881 tggcgtgcac acctcggccg gaattaccgc aaagtggttc tacgaccatc ttgccgatgc
     5941 cggaacgggg tttgaaaaag gcgaccccac cggaaccggt ttcgctgttg acgcaggcat
     6001 cgcgtatcgg ccatcggacc gtctgtactt tggcgcagcc ctcaggaact acggcccgaa
     6061 tgtgcagtac atcgacgcga aacaggcaag cccgacaccg gtgaatttca ccgtcggcac
     6121 ctcatggaaa gccttcgaca cgcaatacaa cgatgttacc atcatggccg acctgtacaa
     6181 gccgctggtg caggactacc gcaagagctg gtatctgtcg atcctgcgtt catgggccga
     6241 cgaatcggtg tacaaagtcg aggaaatacc ggacggacag gggggtgtga cacgcgttga
     6301 gcacaggaac accctgcgtg aagaaacccg tcaggtggat gttctgactg gtgtggagta
     6361 cacgtacgcg gattacgtgg cgatccgagg cggttatttc cgcgattggg atggtcagcg
     6421 caactggatg acattcggcg gaggcctgcg gctgcctatc gcttccacgg ctcttctcgt
     6481 ggatttcgca tacgtgcact cgctgaacgg caagggctca gatccgaacg acggccagca
     6541 ggtatactca atcgggttca cgttctgaaa actctgacgg aacacgacat ttcccgtacc
     6601 ctgcccggcc tgtgctgctg acctgatacc gtcgcgggtt ccgggagcac atgcccggca
     6661 gaaccgactt cacgagtgtc cttcagcata aggtccatca tgcaccaccg ggggctcctg
     6721 ttcgcgtttt tcgccctttc atcgtttctt gcggcagcgt cggcccgagc cgttgaaacg
     6781 attcacctgc ttgccctgcg ggtggagttt ccgaaagagg acccggacga tttcaccacc
     6841 ggcggaaatg gtcttttcga catgcgttcc ggcgcggagg cgctggccac cgcaggagaa
     6901 gcagggttcc ggtacccgtt cgatcttccg ccgcatgacg ggcgctttct ggaaaaccac
     6961 ctgaccgccc tccggaacta cctcgaaact gcctcccagg gacggacgac catcacgtgg
     7021 gaggtattcc caccgcaccc tttgcccgcg tacaaggctc ctcagacgct ggcaagttac
     7081 ggctctggcg gctcggtggc ggagcagacg cggaattggg tacggtttct caaggacgca
     7141 ctggcggtct cacagaatga tgtgggctcg ctggggcggt tccagagctt cctcgtgttc
     7201 catgcaagtg tatcattgca ggggaccctt tccgccgagc ttcccgccct cgtattgacg
     7261 tctcaggaga tcgcctcgtc gggtatcccg attccacccg aggtgcagac ggcatggctc
     7321 attccccagc agattcagca acccggcggc gtaatcggtc tgaatggcgc cttcgcgaaa
     7381 acgtttctcg cctcccgtgg ccttcctgtg ctctcaaaca cacggaccgg tggcgcggcg
     7441 gtgggtgcgt ggacgctgat ggatgtagga tccgacaact tcatccaacg cccgaaaacc
     7501 ggttcttcct ctgatacgat ccgcgtgctg ggtttcgtgc cgtgtctgcc ttctgcgtgg
     7561 gagcagatgc gcctcggctg gcttgcgccg gaaacagttc gctcggacac caccctcctg
     7621 cttgcgggcc ttgccgcaga gacatccctg ccacgcgcat taaaggtgcc catcaccgaa
     7681 gacgaatacc tccttatgga actccgccat tccgcggttg aaacagaccg gtaccacccc
     7741 gcaatcgagt acagcgacgg cgataccggc ggcgtctggc tgaagccaga aaacgggctg
     7801 tatgatgcgt atgtgccggg aagcggggtg atcgtatacc atgtcaacga agcccgcatc
     7861 cgtcagtggg agccgaccaa ttcgatcaat gcccagcccg accggccggg aatcatggta
     7921 gtggaagcgg atgggtatcg cgatatcggc gtgacgaatc ttctggggca cccgcgcgcg
     7981 aatgaaggta tcgggagtcg aaacgacccg tttcccgtta ccggggaacg gatcctgtac
     8041 cccgatggcg agccggcacc cggccatccg gcggtgatca cggcaaacgg tagccccggt
     8101 ggggtcgcaa tttcgttttc gccggcaggt acggcccggg ataccgttgc agtaacagtg
     8161 cgctggcgaa ccgcggacac tcccgcgcga aaaggtcgct tcatcggaag cgctgttgtc
     8221 ggcggagttg ccgtacgcag ggttccgctg gacggtgtgg aacggaacct gatcgcagtg
     8281 gcgggaagcg attggaacct gtgggcgttc accgattccc tcgaacccgt gggcaacgcc
     8341 aacgggttga tcggccgttt ctggggcgat gtcaccagga aaccggtcat cgaagcggac
     8401 ggatccgttc ttgtagtcgg cctcacgacg aacacgcgtt accgatatgc gaacggggcg
     8461 tgggatggcg gtacacctgt ggtgcaaaca ccaccggtgc cgtccacaat gacctgtcac
     8521 cttctgcgcg acgtgcgcaa atcggatatc acagtaacgg gttccgggac tatcactgct
     8581 gatagctatg ctcgcttcgg atacctcccg gaagacccgc ctcccccacg ggtagtctgg
     8641 tcggccacca cggattcgtt gattgcgccg ctgagcctcg gcgacgtaga ccgggacggg
     8701 ttccccgaca tccttgccgt gagccgccgg agcgtggatg tgttctcccg cagtggaact
     8761 cgaacgataa cctatacact cgggcgggcg gattcgaccg aggagtttgc cggatcggcg
     8821 ctctccacgg gagcgggcaa caccattgtg gccgggaaaa aggggctcag cgtgttttcg
     8881 gggctgatgc gtcggcatcc tgcggctaca attccgcttc ccgccgccgc cacgggctcc
     8941 cccgcgttgt acgcggttcg cgatgaaggc atccacgcgg tcgtgggaac cgcggacggc
     9001 tggttgctcg atattcctgt ctcgcagggc gacagcgtca tctggggcca gatgtgggga
     9061 gatggggcgt cgtcgttcgc gctggacgaa acggggcttg taatgccggc agctccggct
     9121 gaggatttga tgcccggaga tcgcgtcttc ttttacccca gccccgtcgg aaaagaagag
     9181 gggatgcttc gattcttcct ttcggcacag gcagatgtct ccgtgcggat attcacgagc
     9241 atgggcgaac aggtctggga gacacgcgtt cccgccggtg agacgattgc aaatgcggag
     9301 aacgaaatcc gctggccggg aggcacacgt ttcgccaccg gtctctacgt tgcccggatc
     9361 accgctcgtt cggcagacgg cagacagggt ggcgccacca ttcccgtcgg catagtgagg
     9421 tgattgcaac ggtgcgtaag ggggtcttgt actccgcttc gaagcggcac gtgtgcggca
     9481 tgtgccttgt cgtgctgcta tttctcgcgg ctccctcgat ttgtgcgacg ccgattgcgg
     9541 aatttgaccc gcccgtgtct gctctctcct ggaaacgatt cgaaaccgag cactttcgca
     9601 tcacatatac ttccggtctt gagcgggtag cgtctgcggt ggcgatggcg gccgagcaag
     9661 cgtatgaacc ggtaacgcgc ctctacgggt atcggccttc gggcaaaacg gaaatcatcg
     9721 tgcgggatca cggtgatttt gccaacggat ttgccgccta ttacgacaac cgcatggaaa
     9781 tctgggcatc tgctctcgac tatgagttcc gcggcactca caactggata tgtgacgtta
     9841 ccacccacga gttcacgcac cttgtccaac tcggtgcgtc gctcaagacc tcgcctgcaa
     9901 tcccacaggt atatttccag tggttccgcc tggaacccga agcgcgaacc gacgtcgcag
     9961 agggacttcc caacacgctc gtgtcgtatg cggtgcccac ggtcagcatc cccatgtggt
    10021 ttgccgaggg cgtcgctcag ttccaggcgc agggggcccg gcatgatcgc tgggacgcac
    10081 accgcgacat gatagtccgc gtcgccgtgc ttgccgacag ccagctctcc taccggcaga
    10141 tggaaggatt ctacgacccg gacgggcgtg aagccgaaat ggtgtacaat cacggctatt
    10201 cgttcgtcag gtggttggcc gcgacatacg gtgattcctc cctccgcgcg ttgagccgcg
    10261 acatgcgtgc gtggcaccgg tgggacttcg gctccgcgct gaaacagctt acaggaaaac
    10321 cgggcccgcg ggtgtacgaa gagtggctga ctcagctccg tgcgggctac gaggcgttcg
    10381 ttgcagagcg aaaaccgcag cccgcgggaa cactgctgga gcggcctgcg tcacgccccg
    10441 gcgcggaatc cggtgaccgc ccgcccgtaa tcaccccggg aggggtaccc gccgcgtacg
    10501 cgccgccacc ggcgcacagc gcgcgggcaa gttattacca tgcagcaccc gtatggtcgc
    10561 cagatggcgc gcttctcgca tacgtctcaa ccgacgggca ggattaccgc ctcggatcgg
    10621 tctgggtccg taaacccggt acaggcgaac tgactgtcgc gccgggatcg ttccgtgcat
    10681 cttcgaccgt aagctggttt cccgacggtc gcacggtcgt gttcagccgc gctcaacgcg
    10741 acgaccgcaa tgagtggcgc tacaacgacc tcgtaaaagc cgaccttcag accggcaccg
    10801 ttacaaagct caccgatcag tggcgcgcct cgtaccccgc cgtctccccg gacggcgcca
    10861 ccgtcgcatt cgtccgaaac gctcgcgggt ccaccaacct catgctcgtg aatgcggacg
    10921 gatccaacgt acgtcccctt acccgttggg atgacggcac gcagattttt tcgccgaagt
    10981 ggtcacctga cggaacgcgt ctcgcagtaa gccttgcccg cgcgggaagc cgccacatcg
    11041 tcgtctaccg tgttgtccgc gcctccgggg gagaggtcct cgaaaccgaa cgcgtaattg
    11101 cttcgtcgga cgatgaccgg gaccccgcct tctcgcgaga tggcacgcga gttgtgtttt
    11161 ccagcgccac cgacggtgtg ttcaacctct atgagcttga tctcgaacgc ggctcatgtg
    11221 cacgattgac aaatgtactg ggcggggcgt ttcacccgga cgtggctcct gacggacgta
    11281 ttgcgtactc ctcgtatgaa tcggcaggct acgtgatccg gatcataccg gcggatgcgc
    11341 cacgatatcc cgtatctgcg gatatatttg cgaatcgtat tccagaacag gggactttcc
    11401 ctgacagtat gcccgaaggt tcctctgtgt cggacattga caggcgcatt ccccatctgc
    11461 agagaccttc aatattgccg cgtttcgggc gctacggcga ccagtggcgt gtgggtgcgt
    11521 acgcgttttc atcggatatc tgggatgatg tccttttgat gggcggagtc tgggccgcac
    11581 cggaaaaccg cgactacgat gcattcgtca ccgcggaagt ggaccacatg acccccgttc
    11641 cggtgctgtt tgatgttgtc aggacggtac gccacacgaa ggaggatacg acctttgcgg
    11701 gtcgtttgcg gctggatggg atcacgtacg gtctgaacgc ggtgtcactc accctgaagc
    11761 cccgcgtgtt ttcccgtgaa atagacctgc attccacgta tcaacgcttc gatgccgcga
    11821 ttgatcagac ccttcttctg aatggaacgc ggacgtatgt gggctacaac tacacgtact
    11881 acaatggctt tgcggcgggt gcctccgtct cttcgtccgc aataagaccg ttcacgaccg
    11941 ctgacattgc cccccagggc atgaagtggt cactccggta tgaccgctgg tggaactggt
    12001 actttgagga tttcgacgcg ggtaccgggc ttctcaacga ggtgtacaca ccctaccact
    12061 acaatcaggt gtcgtgtgac gccgcctggt acctgcccac gccctggcgg gaggaacaca
    12121 ccgtgggcgt ggaagggcgc gtcatgcgta ttgacgctgc ggtggacagc ttcttctatg
    12181 agggtatcgg tggcattatc gggttgcgtg gatacaccta ttaccagctc cagggcaaac
    12241 aaaccgcatg ggggaggttg ctgtaccgat tcccgctgct gacctccatc gaccggaaac
    12301 tggggccggt ctatttcgac aaaatctacg ggaccgtgtt tgcggaggca ggccgcgtct
    12361 ggcgtgactc atacccttcg tcgtggacgc ccggactcaa gcgtgatgtc ggcgcagaga
    12421 tccgcgctga tcttttttcc ttctacgggt acccctcccg gttttccttt tccgccgcgc
    12481 gtgcgctgga tgctgcgcca cattctgaca ggaccaagtt ctaccttacc ttgttgttcg
    12541 ggtatctgta acgggttgcc ccccaacatg acataaggcg gattatcaac acctcctgaa
    12601 cgaatgccga cggcaataat cctgcgaaga cgtatgcctc tcaagtgagc tcaccggctc
    12661 gcctcgccgt tttgtgcgct gacacacacg tagcaaggcc gccgcaatgg cgccgtgctt
    12721 tggagcctgt tcccgcggtg ggtacatttt tccaaggtac cggcgcgaag gtgccgattt
    12781 tgtcggcgtt tttcgcgcgc catcaaggaa acttctccac aatcccgcgc ccgtcgttca
    12841 tgcacaggcg agcagttatt gatgcagcag gggagcaact catgaccgaa catggtaacg
    12901 gacccgaatc aggtctggaa atagatccat ccttactgga tgcggatgcg gtcattcgcg
    12961 cacttcacac ggaccctgaa cacggcctga gctcagcaga agctgcggct cgccttgccc
    13021 gctacggaaa aaacgaactt gtcggcgcgc cgcccgttcc cgtctggcgg aaactgctgg
    13081 cgcagtttga agaccccctt atctaccttc tgctggcggc ggtggcgatt tcggctgtcg
    13141 cgtggttcat ggagggcgcg cacgggtggc ccgtggattc agcagtaatc cttgccatcg
    13201 tagtcgccaa cggcgttctc gggtacgttc aggaggcgaa ggcggaaaac gcggtggccg
    13261 ccctggcgag tatggcggca gcaacctctg cggttgtccg caacggtcag gtggagcgca
    13321 ttccctcatc tgacatagtc ccgggggacg tgttggtact gagtgaggga gacaacatcg
    13381 gcgcggatgg acggctgttc aaaacggcga acctgcgcgt tgcagaggcg cctcttacag
    13441 gcgagagcga gccggttcag aaggaaacat cggcacttca ggaacccagc aaccttggtg
    13501 accggcgcaa tatggtgttc aaaggcactg ccgtgacaca gggagtagga agggccgttg
    13561 tcacggcaac cggaatgaac acagaagtcg gcaagatcgc ccgcatgctt gaaacgacgg
    13621 aagacgaggc gactcccctg cagaaggaaa tcgtgcggct gggaaagatg ctgggaatcg
    13681 ccgtcgttct gatcgccgcg gtcgtcgtca cgacaacgtt cgtggtgttc cgtgtcgaca
    13741 caatggacga ggctgtctcg ctgctgttgc ttggtgtttc acttgccgtg gcggcggtgc
    13801 cggaagggtt gccggcggtt ctttcggtgg ttctggcaat cggcgtgcag cgcatggcgg
    13861 ctcgcaacgc gattgtgaag aaactgtctt ccgcagagac gctcggctcc gcctcggtga
    13921 tctgttcgga caaaactggc acgctgacca aaagcgaaat gacagtgacg aagatcgtca
    13981 cggcttccgg agaggtggcg ttgaccgggg ttgggtacga tccttccggc cgggctgaag
    14041 tcgccgggca ggaaattggc gattcggcgc tgcgcacgga agcacaatgc gtgctcgccg
    14101 gtggcagcct tgctggaaac gcatctcttc gccagcggga cggtgtctgg gagattcagg
    14161 gggacccgac cgaggctgca ttcctcgtgg cagcacaaaa gctgggtggc acgcagcaga
    14221 tcatggagcg ctatgaacgc ctcggggaga tccctttcac ctccgaacgc aagcgcatgt
    14281 ccgttctgct gcgcgaaagg aacaccgggc gcctgacgct gttctccaaa ggagccccgg
    14341 atgtgttgct cgaacggtgc acgcacatcc ttgtgggcaa cgaggtggcg ccgcttgacg
    14401 atgcgcgccg tgcggcaagc gtggagaata tcgaagcact ttccgctcag gcgttcagaa
    14461 ccatcgccgt cgcctaccgg aatctggaag gcgagggagt g
//
LOCUS       DAOTNG010000034        14359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4126_length_14359_cov_5.096686, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000034 DAOTNG010000000
VERSION     DAOTNG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_14359_cov_5.096686"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..702)
                     /locus_tag="P5179_02820"
     CDS             complement(97..702)
                     /locus_tag="P5179_02820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRS94184.1"
                     /translation="MARELGQHGSIPREIPDKTRARRARLFTIANGISLLRVALLPWV
                     LFLISRPPGSHTVELIGLAVVIALTDFFDGFVARRTGQVSHYGRIIDPVADKICLVLA
                     SWWLTVYRGLPVWIPVLVVVRDAVILGGSFVLLRRHDVVLPSNQLGRVTTFVLTITLF
                     AYLIAWEAPQRLLVWLSGALVVATFVSYVRIGWIIFRRFDT"
     gene            complement(702..2441)
                     /gene="recN"
                     /locus_tag="P5179_02825"
     CDS             complement(702..2441)
                     /gene="recN"
                     /locus_tag="P5179_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRS94185.1"
                     /translation="MLEELSVQDFALIERVSLTFSPRFNVITGETGAGKSILVGALGS
                     LLGMRTGAEMVRSGCEKATIEARFRLKRESAVRDLVLEFGGEIEDDTVVIRRLLGSDG
                     RTRVWIGGVSVSVKTLRDVASRLVDFHGQHDHQLLLSPAEHCAILDSFAGTQPLVASL
                     REKLSKLKQLRGQRAALLERQTQSAALRDSEEFERRELDEIDPAPDEYESLLAEQRVL
                     ENAERLGTLLASVCNLLSESDDSVTTRLGQARRWVQEASSIDERLAEEISGDYEALEA
                     LAQEISARCAARVESLEADPGRLDYVSNRLAQLKRLIKRYGSLENAVTRRKELELRVD
                     ADAETAEAIDRLAEEIRCEHREMANLALELSRRRTEAARRLADEVSASLASLGMERAR
                     FEADMVPFSADEVDCLISDAVEYDGQARNVGDSGAEAVEFLIAPNSGEPLKPLARIAS
                     GGEISRVMLAIKSVLAEHDPVSSMVFDEIDAGISGRIGDAVGERMRALAEHRQIIAIT
                     HLPQIAARADHHIVVEKEEARGRTLTLARAAEGEERVLVLAELLGGGEARSTAIEHAR
                     AILNGSEDRGATR"
     gene            complement(2463..3332)
                     /locus_tag="P5179_02830"
     CDS             complement(2463..3332)
                     /locus_tag="P5179_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRS94186.1"
                     /translation="MAIGSVGVVANAEKPNTRDIIRRILKSGERLGFSVVVDAEVADA
                     LGCGSVGVAGEELSKCAELIVACGGDGTILRAARITMGAPRPILGVNLGRLGFLAEAS
                     PEKLEQALEEIDRGDFVTEPRMTLTATVMGTGETVHALNDITIAKSNQSRIISLEMRE
                     AGNWVNTYIGDGLILATPTGSTGYALAAGGPIVKPLAELIVAAPICPHSLTVRPIVFP
                     DDAVLDIRVAGAAESAILLTADGQPTVPLPSGEEVRVRRGAKDVQLVRLASSSYYDTL
                     RQKLHWGLDKNLR"
     gene            complement(3364..5274)
                     /gene="dxs"
                     /locus_tag="P5179_02835"
     CDS             complement(3364..5274)
                     /gene="dxs"
                     /locus_tag="P5179_02835"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRS94187.1"
                     /translation="MPGLLETIDSPLDLRRLPEDQLPELAREIRSLILDVISRNSGHF
                     ASSLGVVELTLALHRVFNTPRDKIIWDVGHQAYVHKIITGRREQFHTIRKKGGLSGYP
                     RREESPYDVFGVGHASTSISAALGLATGRDLRGEDFHVVAVIGDGSMTGGMAWEAINN
                     AGASGRDMLIVLNDNRMSISPNVGALARYFNEIITHETYNKLKADVWDFLGKIPNMGE
                     KVRVGISQLEKAVKSLLVPGIVFEKLGIRYFGPVNGQDVHALIETLEQIKDLKGPRLL
                     HVYTQKGKGVPFAENDPYKWHAGGQFDPRNGEALKKSSPVSYTDVFGKTLTKLCDSHP
                     NVVGITAAMASGCGLKHLADKYPCRFFDVGIAEQHAVTFAGGLALEGTKPVVAIYSTF
                     LQRAYDQIVHDIALQRLPVAFVLDRGGLVGDDGATHNGVFDLSYLRHIPNMVIMAPKD
                     ELEMQRMVLTLVEYNEGPIAMRYPRGCGPGAAIVEEPEALEPIGIGTWEVLAPGTEMV
                     VLAIGSMVYPALEAAQTLREEGMLIEVVNARFAKPLDDGMMEELVRRHRVWVTVEENV
                     LAGGFGSGVMEALECRGLLSSVSLSRLGIHDRFSDHGTREEVLHDEGLDAVSLSEAFR
                     VIRARTLEMKVG"
     gene            complement(5302..5574)
                     /gene="xseB"
                     /locus_tag="P5179_02840"
     CDS             complement(5302..5574)
                     /gene="xseB"
                     /locus_tag="P5179_02840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRS94188.1"
                     /translation="MTETEKPLNFEQAVKRLEEIVHALDDQTLPLDAALALFEEGILV
                     SRQCAAHLEAARARVEKLVEQAPGVFSLEAFDQETSAVDADDEEEE"
     gene            complement(5726..6277)
                     /locus_tag="P5179_02845"
     CDS             complement(5726..6277)
                     /locus_tag="P5179_02845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRS94189.1"
                     /translation="MARLYTRSGDGGRTRLADGSAVPKDDVRVEACGTLDELNAHIGV
                     ARSLLEHSDMDRFLAGVQSTIMELTAGIANPVDAGAGLAEGKIRALEEETDKADGCCP
                     PLRSFVLPAGSSAACALQVARAVCRRAERAIVRLGTEGELPKNALPFINRLSDLLFAY
                     ARWANVRAGAAEELWEAPGKAAH"
     gene            complement(6298..7212)
                     /locus_tag="P5179_02850"
     CDS             complement(6298..7212)
                     /locus_tag="P5179_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS94190.1"
                     /translation="MRIVSLLPSVTRVLYALGAGDDIVGVTYACPQPEGQFKPVVSHD
                     MFATSGYTSEQIAAFLRMLGHQAQGLFTIDEAKLEECAPDVVFLQGACELCVPGTNSN
                     EGTLAGISGNPRAIMIQPHSLGEVMADIRRIGEVANRKPEAETFLSTLKQRMMKVMRL
                     AAGAARGKRRRVLLLEWVDPPTACGLWLPELVDAAGGIPLLGSPQRPAVPTPWEQVLN
                     ANPEVIIVSPAGFALEAAANETELLRKREGWSSISAVASGDVYAVDGTRFFFQPDDRV
                     IDSLELIASILYPEVFGEVHRPDALRLG"
     gene            7413..8519
                     /gene="ald"
                     /locus_tag="P5179_02855"
     CDS             7413..8519
                     /gene="ald"
                     /locus_tag="P5179_02855"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRS94191.1"
                     /translation="MRIGVPKEIKPDESRVALLPSGVHQMVEAGHELFVETNAGRESG
                     YSDSDYQAAGATVVGHASEVFGHADLILKVKEPQQEEIRLIRQGQVVFTYFHFAADRH
                     LVDAMVRSGAVAIAYETVQTEDGELPLLIPMSEIAGRMAIQQGAKYLERRMGGSGVLL
                     SGVPGVAPANVVILGGGVVGSNAAVIAAGMGADVFILDTNLRRLRHLDEVMPPNVRTI
                     MSNHYNIRKLLPIADLVVGCVLIPGAKAPKLVTREMLSLMKPRSVIVDVAIDQGGCFE
                     TSHPTTHEDPVFVVDDIVHYAVTNMPGTVPRTSTIALTNATLPYALKIANEGWKAAAA
                     SSSAIRSGLNIVEGKVVHPAVANAFGLECAEWSP"
     gene            8595..10082
                     /locus_tag="P5179_02860"
     CDS             8595..10082
                     /locus_tag="P5179_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94192.1"
                     /translation="MRDTTSSESLDIHSRSVAQRRLIAEGIFASGQENISGGVILLVY
                     ALALGANTFQIGLISTAGMLGTALQLVADRLLKIAGSRRRVGCAATALLGAGRLSIAL
                     LPYATVWIAAQYLAWGLLAGLVVISGVAQVIEVVRLSWISEVVPENERGRFLGERQLV
                     VQLIGSVIAICAAGFLDWQKGIDAARGLVVCQAYFAIAAFLAVGSIAAFLMMPEPPLR
                     IRNGNGGWHVIVAPFRDAKFFPLMVYSVTWNFAVGFAAPFFNLYLIQVLQMPVSAVAA
                     FNFASQFFSLYCVRLWGRLVDRFGSLPVLRIGVFGKGLYPVAWFLLWPTPETYSTTLL
                     YFLTACVFLFNVLNSANAVSTVSLAMRLMPKDQGTSYLATFRTFANWVHAVSPALAGI
                     ISTGLQGIGWSLQFSIFLLFAISAIGRFAALWTLRFVHEPDARKVSRVFAALKRVPGF
                     SFSHGIMPWFRFWGGPFWAGFAVVKIRMGRMVSTWRGAWPGQDNG"
     gene            10121..11365
                     /locus_tag="P5179_02865"
     CDS             10121..11365
                     /locus_tag="P5179_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94193.1"
                     /translation="METYVELGRAIPVTGTFDVVVVGGGCAGFAAAVASARCGAQTLV
                     IERFPYFGGTATASLMANINGFRNQVEPDSVQTVKGIPAEVIRDLHELGGLGKSPYPQ
                     KEYPIEKGTLSYSYAIDTEAFKYVTLRKAVEAGVRILFHTFFVDAIKEANSVRGVIVE
                     NKSGRQAIFARVVVDASGDADVAARAGAQFWQTKQSENKRLTDQLMYRIAGVSEGSDI
                     GGCRARNGVTVWGPGSFVQDGTDAEAVTRSEISARLAVWEHFQTLKTKHTGMEAAYIA
                     ETPPLIGIRQTRFVRGLYSLTADDAISGRRFDDVVAISSCPIISYYGYRRYLEHEGFD
                     IPFRCLVPLELDGLLIAGRCISSEQEPYESFRAMAPIMAIGQAAGAAAALASKSSVKV
                     RDVEISRLQSLLVEQGAELRLA"
     gene            11416..12213
                     /locus_tag="P5179_02870"
     CDS             11416..12213
                     /locus_tag="P5179_02870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRS94194.1"
                     /translation="MNHHRPKRAFERAEFLSSAEGRGIRILSEYLEPEQRLNALRIWH
                     TAVFFGSARLCDRETAARQLAELERRNAAEDEIAAARVDLEMSRYYEDARALAAMITN
                     WSSTLPEEERLYVCSGGGGGIMEAANRGASEAGGRTVALNIQLPFEQVANPYTQEELT
                     FDFHYFFMRKFWFVYRAKVIIVFPGGFGTLDELLESLTLTQTGRVEKRMPIVIYGKEF
                     WNSVINFSVLAEKRVISPSDLELFHFSDTPEDAFAHVCEKLSFDAGT"
     gene            12275..13369
                     /locus_tag="P5179_02875"
     CDS             12275..13369
                     /locus_tag="P5179_02875"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS94195.1"
                     /translation="MPRANSEAVRERQARAKRYAARRVDYPRKVMDLWKGVMRERFLR
                     TRELANFRGFRGDFHTHSNHSDGAGTVAELAHFRDAAGLDFLFVTDHSGLSQKRDCLR
                     FERMWWGQEPGTQHHHLGILGLTRKFVPAKDLMRDYAAIKRRGGIPFIPHPCGWFPTT
                     RYTEEQIAALDTLDDAFIMEIVNGANQIFDCWDVTDAQSVELWDRLLCAGKRVTALGN
                     TDAHLAEAIGDIWTGVLAGECTHTAILDALRMGRVFVSDAPVIDISVQCDGSEPVGMG
                     GTLFVPPSKRNSPLVVRILAADSAGLASVSLVGGGMVLESWNVADKAVLSVSFVVPTR
                     QEPVYFRCECRSTDGRRAFSNAVYVVRKTE"
     gene            13379..>14359
                     /locus_tag="P5179_02880"
     CDS             13379..>14359
                     /locus_tag="P5179_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94196.1"
                     /translation="MRKYWNPFKPPVKRRKPIERSSRRSASFDPKNQPKRTADLLRLE
                     KEYIRPYWGRMVLAILISTVSSITPFAFGYTARFIVDRILELHRWKGSAIIHVPAAEM
                     PEKMKLLFIVLAANLGLHLLAIVCSWIFHYNAVLVAQRIVFTLRKQLYEKLQKLQITY
                     FDQRITGKIMARVLDDVSVIQGNVTSTFVSIVTNVMMFLVGVVILFSINAKLAAIAII
                     IVPTWAIVYRFFHKAIIQNHRHLREKNSEIYGVVEEKISAIRVVKAFAAERREDRRYM
                     HLAADFVRLGVRQTKLSNGLTFLASLISVFGTSLILYLGALYVRDRVITVG"
BASE COUNT         2943 a         4322 c         4075 g         3019 t
ORIGIN      
        1 cgtgccgagt tcctcaagaa gatcgaccag acgggagcgc gctttgtatt caaagtccgg
       61 cagacggtag gccaaggaag agcccctttg tggcgctcaa gtgtcaaagc gccggaaaat
      121 gatccaccct attcggacgt acgacacgaa tgtcgccact acaagcgcgc cgctcaacca
      181 gacaagaagc cgttgaggtg cctcccatgc gatgagatag gcgaagaggg taatcgtgag
      241 cacgaaggtc gtgacccggc ccaactggtt tgacggcaac acgacgtcgt gtcgccgcag
      301 caacacgaag ctccccccga gaatcaccgc gtctctcacc accaccagaa caggtatcca
      361 gacgggtaac cccctgtaca ccgtcagcca ccacgaggcg aggaccaggc agatcttgtc
      421 ggcaaccggg tctatgatgc gtccgtaatg tgaaacctgc ccggtgcgcc gcgcgacgaa
      481 gccgtcaaag aagtcggtca gagcaatcac gacggcaagc ccgatcagtt ccaccgtgtg
      541 ggaaccggga ggacgcgaaa taaggaataa cacccatgga agcaacgcga cgcgcaggag
      601 actgatgccg ttggcgatgg taaaaaggcg cgcacggcgc gccctcgttt tgtcgggtat
      661 ctctcgaggg atcgaaccgt gttgcccgag ttcccgcgcc attaccgcgt cgccccacga
      721 tcttccgacc cgttcaggat cgcacgcgcg tgctcgatgg cagtactccg agcctcccct
      781 ccgcccagca actctgcaag caccagcacg cgctcctctc cctccgctgc gcgcgcgagt
      841 gtcagcgttc gcccgcgagc ttcttccttt tccaccacga tgtggtggtc ggcgcgagca
      901 gcgatttgtg gaaggtgcgt aatcgcgatg atctgccggt gttccgcaag agcccgcatt
      961 ctttccccga cggcatcgcc gattcgtccc gatattcctg cgtcgatctc atcgaacacc
     1021 atggaggaaa ccgggtcatg ctcggcgaga acgctcttga tagccaacat cacacgagaa
     1081 atctcacctc cggaagcgat acgggcgagc ggcttcagcg gttctccgga attcggcgcg
     1141 atcagaaact ccaccgcctc ggcacccgaa tcacctacgt tccgggcctg tccatcatac
     1201 tcaacggcgt ccgaaatgag acagtccacc tcgtctgcgg agaaaggaac catgtccgct
     1261 tcaaagcgcg cacgttccat tcccagtgac gcaagcgacg cgctgacctc gtcggctagg
     1321 cggcgtgccg cctccgtcct cctgcgtgat agctccaggg ccagattcgc catctcgcgg
     1381 tgttcgcaac ggatttcctc agccagccga tcgatcgctt ccgccgtttc cgcgtcggcg
     1441 tcgactctca gttcgagttc ttttcttcgc gtgaccgcgt tttccaggct cccgtatcgt
     1501 ttgatcaagc gtttcagttg agcaagccgg ttggatacgt aatcgagcct cccggggtca
     1561 gcttccagag actccacccg cgctgcgcac cgagcggaga tttcctgcgc gagagcttcc
     1621 agcgcttcgt aatcgccact gatttcctcc gcgagccttt cgtctatgga ggacgcctcc
     1681 tgaacccacc tccgcgcttg ccccaggcgg gttgtcacgg agtcgtccga ttcgctcaga
     1741 aggttgcaca cgctggcaag gagtgttccg aggcgttccg cattttcaag tacgcgctgt
     1801 tcggccagaa gggactcgta ttcgtcgggc gcggggtcta tctcgtccag ttccctgcgt
     1861 tcgaactcct ctgaatccct cagcgcggca ctctgggttt gccgttcaag gagagctgcc
     1921 cgttgcccac gcagttgttt cagcttcgaa agcttctctc gcaacgaggc cacaagcggc
     1981 tgcgtacccg cgaaggagtc cagtatcgcg cagtgttcag ccggcgaaag aagaagctgg
     2041 tgatcatgtt gtccgtgaaa atccacgagg cgggaagcca cgtcacgtaa cgtcttgacg
     2101 gaaaccgaaa caccccctat ccacacgcgc gtccgcccat cactccccag caggcgacga
     2161 atgaccaccg tatcgtcctc gatctccccc ccgaattcga gcaccagatc gcggacggca
     2221 ctctcccttt tcagccggaa ccgtgcttca atggttgcct tctcgcatcc ggagcggacc
     2281 atctccgcgc ccgtacgcat tccgagcagg gaaccaagcg ctcccacaag gatcgacttg
     2341 cccgcacctg tttcaccggt gatgacattg aaacggggag agaaggtcag ggatacacgc
     2401 tcaatcagcg cgaaatcctg aaccgacagt tcttcaagca tcggcccgct ctggcctcgt
     2461 ggctaccgaa ggttcttatc gagtccccaa tgcaacttct gccgcagcgt gtcgtagtac
     2521 gacgacgagg ccaaccgaac aagctgaacg tctttggccc ctcttcgcac acgcacttct
     2581 tctcccgagg gcaggggtac cgtgggctgc ccgtcagcgg tgagcaatat cgcgctttcg
     2641 gcagcccctg ccacgcgaat gtcgagtacc gcatcgtcgg gaaacacaat cggacgcacg
     2701 gtaagtgagt gggggcatat tggtgccgcg acgatgagct ccgccagagg cttgacgatg
     2761 ggtcctcctg cggcgagcgc gtaccccgtg gaccccgtgg gtgtcgcaag aatcaacccg
     2821 tcgccgatgt acgtgttcac ccagttgccc gcttcgcgca tctccagcga gataatccgg
     2881 gattgattgc tttttgcgat agtgatgtcg ttcagagcat gaacggtttc gccggtgccc
     2941 ataactgttg cggtgagagt cattcggggt tcggtaacga aatcgccgcg gtcaatctcc
     3001 tcaagggcct gttccagttt ctccggtgaa gcttccgcaa ggaatccgag gcgccccaga
     3061 ttcactccca gtatgggacg cggggcaccc attgtgatcc gtgcagcccg caggatcgtc
     3121 ccgtctccgc cgcaggcaac gatcaactcg gcgcattttg acagctcctc gcctgccacg
     3181 cccacggatc cgcacccgag ggcatcggcg acctctgcgt caacaactac cgaaaacccg
     3241 agccgttcgc cggatttaag gatccgccgg atgatatcac gcgtgttcgg tttctccgcg
     3301 ttcgccacaa ctccgacgga tcctatcgcc atcccctacc ccgcattcgc gaaccggtac
     3361 aaatcacccc accttcatct ccagcgtcct cgccctgatc acgcggaatg cctctgagag
     3421 gcttaccgca tcgaggcctt cgtcgtgaag cacctcttcg cgagtaccgt ggtccgaaaa
     3481 acgatcgtga atgccaagtc gactcagtga aaccgatgaa agcagtccgc ggcattccag
     3541 agcttccatt acgcccgatc cgaaaccccc ggccaatacg ttttcttcaa ccgtcaccca
     3601 gactctgtgc ctgcgcacaa gttcttccat catcccgtca tccagaggtt tggcaaaccg
     3661 cgcattgacg acttcaatca acataccctc ttctctgagg gtctgagcgg cctcgagcgc
     3721 gggatacacc atgcttccta ttgcaagcac gaccatctct gtccccggcg cgagcacctc
     3781 ccacgtcccg atcccgatcg gttcaagtgc ctctggttcc tcaactatgg ccgcacccgg
     3841 accgcaaccg cggggatacc tcatggctat gggcccctca ttgtattcga cgagcgtgag
     3901 caccatccgc tgcatttcca gctcgtcttt gggtgccatt atcaccatgt tcgggatgtg
     3961 gcgaaggtag cttaagtcga aaacgccgtt gtgcgtggcc ccgtcatccc ctaccagccc
     4021 cccacggtca aggacgaacg ccaccgggag gcgttgcagc gcgatgtcgt ggacgatctg
     4081 atcataagcc cgttgcagaa atgtgctgta gatggcgaca acgggttttg tgccctccag
     4141 cgcgagcccg cccgcgaacg tcactgcgtg ttgttccgcg atgccgacat cgaagaaacg
     4201 acacgggtat ttgtcagcaa gatgcttgag cccgcacccg gaggccatcg ccgcagttat
     4261 ccccacaaca tttggatgtg aatcacaaag cttcgtcaac gtcttgccaa acacgtccgt
     4321 gtacgacaca ggtgagcttt tcttgagcgc ttctccattt ctcggatcaa actggccgcc
     4381 ggcatgccac ttgtacggat cgttttccgc gaaaggaaca cccttcccct tctgcgtgta
     4441 cacgtggagc aggcgcggac ctttcaggtc cttgatctgc tccagagttt cgatgagcgc
     4501 gtggacgtcc tgtccgttga cagggccgaa gtaccgaatc ccgagcttct cgaagacaat
     4561 cccgggcaca agcagcgatt tcaccgcctt ttccagctga cttatcccaa cgcgaacttt
     4621 ttcgcccatg ttcgggattt ttccgaggaa atcccaaacg tctgccttga gtttattgta
     4681 ggtctcgtgc gtgatgattt cgttgaaata ccgcgccagc gctccgacgt tgggcgaaat
     4741 agacatgcga ttgtcgttca agacgatgag catgtcccgg cctgaggcac ccgcgttgtt
     4801 gatcgcttcc catgccatgc cgccggtcat tgatccgtca ccaatgacgg ccaccacgtg
     4861 gaaatcctcg ccccggaggt ctctgccggt agccaggccg agcgccgcgc tgatgctcgt
     4921 ggaggcatgg cctaccccga atacatcgta ggggctttcc tctcgccggg gatagccgct
     4981 caatcctccc tttttccgga tggtgtgaaa ctgctcacgc cgaccggtga tgatcttgtg
     5041 gacgtacgcc tggtggccca cgtcccagat gattttgtcg cggggagtgt tgaacacacg
     5101 gtgcagtgca agcgtaagct ccaccacacc gaggcttgat gcgaaatgac cgctgttccg
     5161 cgaaatcacg tccagaatga gtgagcggat ctcgcgtgcc agttcaggca attggtcttc
     5221 cggcaaccgc ctgaggtcaa ggggtgagtc tatggtttcc aacaagccag gcatctggtc
     5281 tccagaaatt ctcgcagtgt tctattcctc ttcttcgtca tccgcatcaa cggcggacgt
     5341 ctcctggtcg aacgcttcca gactgaacac tccgggagcc tgttcgacga gcttttccac
     5401 ccgcgctctc gccgcttcga gatgtgccgc gcactggcgg gaaaccagta tcccttcctc
     5461 gaaaagagcc agcgctgcgt caagcggcag cgtctggtcg tcaagcgcgt gaacgatttc
     5521 ctcgaggcgc tttacggcct gttcgaagtt caaaggtttt tccgtttccg tcacggcaac
     5581 acctcggggc ataacggcgg gcaacccggc aaaggccgcc caggatgggt gatttgttaa
     5641 cgtaaatgta caaccgcagc tgtccaacgg cgaggacaag tcgtccaagt cgttgggcac
     5701 ttcgaaacgg cctccgagga agcactcagt gtgccgcttt gcccggggct tcccacagtt
     5761 cttcggcggc accggcgcga acgtttgccc accgcgcata ggcaaaaagg agatcagaaa
     5821 ggcgatttat gaacggaagt gcgttttttg gaagttcccc ttcagttccg agcctcacaa
     5881 ttgcacgctc tgctctcctg cacacagctc gagcgacctg cagggcacac gcggccgaag
     5941 acccggcagg aaggacgaag gaacgcagag gtggacaaca accgtccgct ttgtccgttt
     6001 cctcttccaa tgcacgaatc ttcccttccg ccaatcccgc tcccgcgtcc accgggttgg
     6061 caattcccgc ggtcaattcc atgatggttg actgaacgcc ggcaagaaag cggtccatgt
     6121 cgctgtgttc cagaaggctc cgcgcaacac cgatgtgcgc attcagctca tccaacgtgc
     6181 cacacgcctc cacacgaaca tcgtctttcg gcactgcgga accgtccgcc agtctggttc
     6241 tccccccgtc cccggatcgc gtgtaaagcc ttgccatatt gtcctcatca tccctgacta
     6301 accgagccgc aacgcgtccg gccggtgcac ctcgccgaaa acctcggggt aaagaatgga
     6361 ggcgatgagc tccaggctgt cgatcacacg atcatccggc tggaagaaaa atcgtgtccc
     6421 atccacggca tacacgtccc cggaagctac ggccgaaatg gacgaccacc cttctcgttt
     6481 gcgcagcaac tcggtttcat tcgccgctgc ttccagcgcg aatccggcgg gcgaaacaat
     6541 gatcacctcc gggttcgcgt tcagcacctg ttcccacggc gtgggtaccg ccggtctctg
     6601 gggtgacccc agcaggggaa tcccgccggc agcgtccacc agttccggca gccagagccc
     6661 gcatgcggtg ggagggtcta cccattccaa cagaagaacc cttctccgtt ttccacgggc
     6721 cgcacccgcc gcaaggcgca tgactttcat catgcgctgc tttagtgtcg aaagaaacgt
     6781 ctcagcttcc ggtttcctgt tagcaacctc gcctattcgt cggatatctg ccataacctc
     6841 gccgaggctg tgtggctgga tcataattgc ccgagggtta ccggagatac cggccagcgt
     6901 cccctcgttc gagttggtac ccggtacgca gagttcgcac gccccctgga gaaagacaac
     6961 gtccggcgcg cattcctcca gtttcgcctc gtcaatggtg aacaggccct gagcctgatg
     7021 ccccaacatc ctgaggaacg cagcgatttg ttcgctcgtg taacccgagg tcgcgaacat
     7081 gtcgtgcgac acaactggtt tgaattgccc ttccggttgg ggacacgcgt aggtaacccc
     7141 tactatgtca tcccccgcgc caagcgcata cagaacgcgc gtgacactgg gcaataacga
     7201 tacaatgcgc atttcatctg ctccgtgatt tcggctcgaa actccaatcc agaaagacgt
     7261 tcagattaca catcgagaac cagttcttcc aagaccggtg cgtgaaaaca ggtaccgtaa
     7321 tatattcacc attgcgtttg catttggcat tcaggagcac ccggcaacag tgacaaatac
     7381 tgacacgtgt ttgcggaatg aggaggcgcg ccatgagaat tggtgtcccg aaagagatca
     7441 agcccgacga aagccgggtg gcgttacttc cctccggagt acaccagatg gtggaagccg
     7501 gacacgagct tttcgttgaa acgaatgcgg ggagggaaag cggctattct gacagcgact
     7561 accaggcggc cggcgcaact gttgtaggac acgcttccga ggttttcggg cacgcggacc
     7621 tcatcctgaa agtcaaagag ccgcagcagg aggaaatccg cctcatccgg caggggcagg
     7681 tggtgtttac ctacttccac ttcgccgcag accgccatct cgtggacgcg atggttcgca
     7741 gcggtgcagt ggcaatcgcg tacgaaacgg ttcaaaccga ggacggtgaa ctgccgcttc
     7801 ttattccgat gagtgaaata gcaggacgga tggcaatcca gcagggcgcg aagtacctcg
     7861 agcgccgcat gggcggtagc ggcgtcttgt tgagtggcgt gcccggtgtc gcgccggcaa
     7921 atgttgtcat tctgggcggc ggcgttgtcg gttccaacgc cgccgtgatt gcggccggga
     7981 tgggagccga cgtgttcatt ctggatacca atctccgccg gttgcgacat cttgacgagg
     8041 taatgccgcc gaacgttcga acaataatgt cgaaccacta caacattcga aagctccttc
     8101 ccatcgctga cctcgtggtg gggtgcgtgc tcattccggg cgcaaaggct ccgaagcttg
     8161 tcaccaggga gatgctgtcg ctcatgaagc cacgctcagt tatcgtggat gtggcgattg
     8221 atcagggggg ctgttttgag acgtcccacc cgaccacaca cgaggatccc gtttttgtgg
     8281 ttgacgacat cgttcattac gcagtgacga acatgcccgg gaccgtgcca aggacctcca
     8341 cgatagccct gacaaatgca acgttgccgt acgccctgaa aatcgcgaac gagggatgga
     8401 aagcggccgc agccagcagc agcgccatcc gcagtggcct taacatcgtg gaaggcaagg
     8461 tggtccatcc cgcggttgcc aacgcattcg gactcgaatg tgctgagtgg agcccctgac
     8521 acccaacggt ttggtcagga ccggacccgg cgtacctttt ttgccagacg aacacggttc
     8581 tccggatctg gtgaatgcgc gatacgactt cttctgaatc tctcgacata cattcccgtt
     8641 ccgtcgcgca gcgaaggctc atcgcagagg gtatcttcgc gtccggccag gaaaacattt
     8701 ccggcggcgt gatccttctt gtgtacgccc ttgcgctggg cgcgaacacc ttccagatcg
     8761 gcctcatctc taccgccggg atgcttggaa cggcgctgca acttgtggcg gaccgtctcc
     8821 tgaaaatcgc aggcagcagg aggcgagtag gctgcgcggc gaccgcgttg ctgggagctg
     8881 gacggctttc gatcgcgttg ctcccgtatg cgactgtatg gattgccgca caatacctcg
     8941 catggggtct gcttgccggt ctggtcgtca tcagcggcgt tgctcaggtt atcgaagtcg
     9001 tccgcctctc gtggatttcg gaggtcgttc cggagaacga acgcggacga ttcctcggcg
     9061 aacgtcaact cgtcgtgcag ctgatcggaa gcgtgatcgc catttgcgcc gctggcttcc
     9121 tcgactggca gaagggaatt gacgccgcgc gcgggttggt cgtgtgccag gcctatttcg
     9181 cgatagcggc gtttctggcc gttggcagca ttgcggcgtt tctcatgatg cccgagcctc
     9241 cgctgcgaat cagaaatggg aacggcgggt ggcacgtcat cgttgccccg ttcagagacg
     9301 cgaagttctt cccgctcatg gtctactccg tgacgtggaa tttcgcggtc ggattcgccg
     9361 ctccattctt caacctgtac ctcattcagg tgctgcagat gccggtgagc gccgtggccg
     9421 cgttcaattt cgcgtcgcag ttcttctccc tttactgcgt gcgtttgtgg gggcgccttg
     9481 tcgatcggtt cgggagcctt cccgtcctgc gcattggtgt gtttggcaag gggctatatc
     9541 ccgttgcatg gtttcttctc tggccgacgc cggagacgta ctcaaccacg ctcctgtatt
     9601 ttctgaccgc atgcgtgttc cttttcaatg tgctcaactc cgcgaacgcc gtttccacag
     9661 tgagtctcgc gatgaggctc atgccgaagg atcagggaac cagttacctt gcgacgttcc
     9721 ggactttcgc caactgggta cacgcggtca gtcctgcgct cgccgggatc atctccacgg
     9781 ggctccaggg aatcggctgg tcgctgcagt tcagtatatt ccttctgttc gcgatttctg
     9841 ccatcggtcg gttcgctgcg ctgtggaccc tccggtttgt gcacgaaccg gacgcaagaa
     9901 aagtctcgcg cgttttcgct gcactgaaac gcgttccagg tttttccttt tcgcacggca
     9961 taatgccatg gttccgcttc tgggggggac ccttctgggc gggtttcgcc gtggtcaaga
    10021 ttcggatggg gcgcatggtt tcgacatggc gaggtgcgtg gcccggacag gacaacgggt
    10081 aacagggttt tgaccgtcgc caacgcggag ggaaagtgcc atggaaacat acgtcgaact
    10141 cgggcgggca atccccgtga cgggcacctt tgacgttgtg gttgtcggcg gagggtgtgc
    10201 gggtttcgcc gcagccgtgg cgagtgcaag gtgcggcgcg cagactcttg ttattgagcg
    10261 ttttccgtat ttcggtggga ccgctaccgc gtcgctcatg gcgaacatca atggtttccg
    10321 gaaccaggtg gaaccggatt cagtgcagac ggtcaaaggg attccggctg aggtgatccg
    10381 ggacctgcac gagctcggcg ggttgggcaa aagcccgtat ccgcagaagg aatacccgat
    10441 agagaaagga acgctttcct actcgtacgc aatagacacc gaggcgttca aatacgtgac
    10501 gctgcgaaaa gcagtagaag caggagttcg tatccttttt cacacgttct tcgtagatgc
    10561 gatcaaggaa gcgaactccg tccgtggcgt aatagttgaa aacaagtcgg gaaggcaggc
    10621 gatcttcgcg cgggtagtgg ttgatgcaag cggcgacgca gacgtggcgg cgcgagcagg
    10681 tgcccaattc tggcagacga agcaatcaga aaacaaacgt ctgactgacc aactgatgta
    10741 ccgcatcgcc ggagtctcag aggggagtga tatcggtggg tgcagggcgc gcaatggcgt
    10801 tactgtgtgg ggaccgggat cctttgttca ggacgggacg gatgcagagg cggttacccg
    10861 ttcggagatt tctgcgcgac tggcagtgtg ggagcatttt cagacgctca aaaccaaaca
    10921 taccggaatg gaagcagcct acatcgccga aaccccgccc ctgattggaa tcaggcagac
    10981 ccggttcgtg cgcgggttgt attccctcac ggcagatgat gccatatcag ggcgcagatt
    11041 cgatgacgtg gtggccatca gttcatgccc gataataagc tattacgggt acaggcgtta
    11101 tcttgaacac gaaggatttg acattccgtt ccgttgtctg gtacctcttg agctcgatgg
    11161 gctgctgatt gccggccggt gcatttcctc cgaacaggag ccttacgaat ccttccgggc
    11221 gatggcaccg atcatggcga tcggccaggc cgccggagcc gcggctgccc tggcgtccaa
    11281 gtcatccgtc aaagtgagag acgtcgagat cagcagactt caatcgttac tcgtggaaca
    11341 gggagcggaa ttgcggcttg catgatcgca tccgaaggtt ttcggcgaag cgaacgaaca
    11401 acgaaggatc gcacgatgaa ccatcatcgc ccaaaaagag cattcgaacg agctgaattc
    11461 ctttccagcg ccgaaggccg cggaatcagg attctgagcg agtaccttga accggagcaa
    11521 aggctgaacg cgctgcgtat ctggcacacg gcggtgttct tcggttccgc gagactgtgc
    11581 gaccgagaaa cagccgcgag gcagcttgcc gagctggaga ggcgcaacgc cgcagaggac
    11641 gagatcgcgg ccgcccgagt tgacctggaa atgtcccgtt actacgagga cgctcgtgcg
    11701 ctggccgcga tgatcacgaa ctggtcgagt acactcccgg aggaagaacg cctgtacgtc
    11761 tgctccggcg gaggcggcgg aatcatggaa gccgctaaca ggggagcctc tgaggccggt
    11821 ggacgaacag tcgcgctcaa cattcagctc cctttcgagc aggttgccaa cccgtacaca
    11881 caggaagagc tgacgttcga tttccactac ttcttcatgc gcaaattctg gttcgtgtac
    11941 cgcgcgaaag tcataatcgt gtttcccgga ggctttggaa cgctcgacga gttgcttgaa
    12001 tctctcacgt tgacccagac cggacgggtg gaaaaacgca tgcccatcgt gatttatggc
    12061 aaggaattct ggaacagcgt gatcaacttc tcggtccttg ccgagaaacg agtcatctcg
    12121 ccctccgatc tcgagctttt ccatttttcc gataccccgg aagacgcgtt cgcgcacgtg
    12181 tgcgaaaaac tcagtttcga tgccggcacc tgagacgact gaaaaccgcg tggaacgggt
    12241 gaggcaatcg agcaccgacc gaaaggaagg caatatgccc cgggcgaact ctgaagctgt
    12301 tcgggaacga caggcacgag ccaagcggta cgcagccaga cgggtggact acccgcgcaa
    12361 agtgatggac ctctggaaag gggtcatgcg ggaacggttc ctgcggaccc gcgagcttgc
    12421 gaatttccga ggctttcgtg gtgacttcca tacccactct aaccactccg acggggcggg
    12481 aacagtggct gagctcgcgc atttccgcga tgcggccggc cttgattttc tctttgtcac
    12541 cgaccattcc ggcctttccc agaaacgcga ctgcctcaga tttgagcgga tgtggtgggg
    12601 gcaggaaccc ggaacacagc atcaccatct cggcattctt gggctgacaa ggaaattcgt
    12661 tccggcaaag gacctgatgc gtgattatgc tgccatcaag cggcgtggcg gcattccatt
    12721 catcccccac ccgtgcggct ggtttcccac cacacgttac accgaggaac agatcgccgc
    12781 gctggatacg ctggatgacg ccttcatcat ggagatcgtg aacggcgcca accagatttt
    12841 cgactgctgg gacgtaaccg acgcacaatc ggtggagctc tgggataggc tcctgtgtgc
    12901 cgggaagcgt gtcacagcgc ttggcaacac ggatgcgcat cttgcggagg cgattggtga
    12961 catttggact ggggtgcttg ccggggagtg tacgcacaca gcgattcttg acgccctgcg
    13021 catgggacgc gtgttcgtgt ccgacgcccc tgtaatcgat atctctgttc aatgtgacgg
    13081 ttctgaaccc gtcggaatgg ggggaacact cttcgtccct ccttcaaagc gcaattcgcc
    13141 gctggtcgtg cggattctcg ccgcagacag cgcaggactt gcttcggttt ctctcgtggg
    13201 tggcggcatg gtcctggaat cgtggaacgt ggctgacaag gccgttctga gcgtttcgtt
    13261 cgttgtgcca acccgccagg aaccggtata ttttcggtgt gaatgccggt cgaccgacgg
    13321 gcggcgcgca ttcagcaacg ctgtgtacgt ggtaagaaaa accgaataaa gcctttcgat
    13381 gcgaaaatac tggaacccgt tcaaacctcc ggtcaagcgc cgcaaaccta tcgaacgctc
    13441 ctcgcggcga agcgccagct tcgacccgaa aaaccagccg aagagaaccg ccgacctttt
    13501 gcgtctggaa aaggagtaca tccggccata ctggggccga atggtactgg cgattctgat
    13561 cagcacggtc agttccatca cgccgttcgc gttcggttac accgcgcgtt tcatcgttga
    13621 ccgaatcctc gaacttcacc ggtggaaggg ttctgcgata atccatgttc cggctgccga
    13681 aatgccggaa aaaatgaaac tgctcttcat cgttcttgcg gcgaatcttg gcctgcacct
    13741 gctcgccatc gtatgtagct ggatattcca ttacaacgcg gtgcttgtag cgcaacgcat
    13801 agtgttcact ctccgaaaac agctctacga aaaactccag aagctgcaga tcacctattt
    13861 cgaccagaga atcaccggga aaatcatggc acgcgtgctg gatgatgtgt cggtcatcca
    13921 ggggaatgtc accagcacgt tcgtaagcat cgtcaccaat gtcatgatgt tcctcgtggg
    13981 tgttgtcatc cttttttcga tcaacgcgaa actcgccgcc atcgcaataa tcatcgtacc
    14041 tacctgggca atcgtctatc gattttttca taaagccatt atccagaacc accgtcatct
    14101 ccgcgaaaag aactcggaga tttacggggt cgttgaagag aaaatcagcg cgattcgcgt
    14161 ggtgaaagcg ttcgcagccg aaaggcgaga agacaggcgt tatatgcacc ttgccgcgga
    14221 tttcgtccgc ctcggggtgc ggcagacaaa gctttcgaac ggtctcacat tcctcgcaag
    14281 ccttatcagc gtgttcggca catccctgat cctgtatctt ggggcgctgt acgttcgcga
    14341 ccgcgtgata accgtgggc
//
LOCUS       DAOTNG010000035        14203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4188_length_14203_cov_5.238479, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000035 DAOTNG010000000
VERSION     DAOTNG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_14203_cov_5.238479"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="P5179_02885"
     CDS             <1..984
                     /locus_tag="P5179_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRS94197.1"
                     /translation="IFLCAHNAGHSLSEAVLSMDSAAHGRTSGDGRMPWYYGARLYVY
                     ILPTFVFIGIFAYYPSLSAIYHSFFNWNGSNIKDYVGLANFRRIFLNDRDLLVASWHM
                     LLFVSANLVLQIPSILLAVVLYHLRNKRVQYWFRMVFVIPMVVPGMVGMLLWMFIYNP
                     QVGLLNRALAGLGIIPSPFDGPTWLADTRLAMPSLMFMGFPYINPIALLIYLAGLERI
                     PRDLNDSALIDGASAWQRLVRVELPMILGQVKLMLILGIIGGVQDYGRVLIMTGGGPS
                     DATTVPGLIMYNTAFRYAQMGYASAIGVVMFLVILGLTVLNMRYFRTHEAS"
     gene            1029..1847
                     /locus_tag="P5179_02890"
     CDS             1029..1847
                     /locus_tag="P5179_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRS94198.1"
                     /translation="MALTSRRAEAAKFTLLAGAAFFVLVPFWVMISISLKDLRQFLDN
                     ILYPTSPFQWQNYVEGWEAVSPYIINTLVVAAVVTVVSLTLSSLTAYVFARYEFPGKT
                     PLFYALIVLLMIPGILNLIPQYVLALRLGLVGTAWALILFYVASRQVLEVYILRTFFS
                     GLPKELFEAAQMDGASDIRQYWSIALPLSKSILGTLAIMTVVFVWNDYIWPLVAADNF
                     KTVSIGLAYLTKQHSTSWGPLMAGYAIASLPLILLFSVTMRYFVEGIATGAIKA"
     gene            complement(1906..3318)
                     /locus_tag="P5179_02895"
     CDS             complement(1906..3318)
                     /locus_tag="P5179_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94199.1"
                     /translation="MFSLNARMARPRIGLLPTGHYYYWQQFPELRAKGLAMYGRLLER
                     LGTFGDVIAPELVDTPEKSAAAGELFLKERVDIVLVFPFGYTPAMCVVPAARSLSVPI
                     RILNAHEDASYDYAKADTTEYLHHEGVCCVPEYASALRTIGKSFRVRTGAFRDGRLWD
                     EVAADCRGAAAARVFRALNFGVIGETYTNMADMPVDELRLLNATGRLLIRPEVEEIEE
                     AYHHATRAQVDEMCGQFRAMYDVDAGVTDEHLRESARIAVAFDEVIMHHDLSAFGFYW
                     WGMKEAVTQLRAQAALAVSRLAALGRPGVTEGDVKTAMAMKLLDLLGAGGMFVEFFSM
                     DFDENFILMGHDGPSNINVAQDRPKLSFLEVHHGKSGSGLGIDFHMKEGPVTLVNLTQ
                     VDAGESFKLITTAGEVVPGNILSIGNPNCRVRVERPIHEFMDAWCQQGPSHHIALGIG
                     DHTAEVEAFAEAMGFRYVRV"
     gene            complement(3557..4504)
                     /locus_tag="P5179_02900"
     CDS             complement(3557..4504)
                     /locus_tag="P5179_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS94200.1"
                     /translation="MSPRTVSIVIPLLDEEESLRPLHEAITRVVDREGYVAEIVFVDD
                     GSRDGSFSVIRELAEKDPRVKAVRFRRNFGKSAGLATAFRVATGDIVVTMDADLQDDP
                     EEIPNLIAKLDEGYDLVTGWKVIRHDPISKTLPSKVFNGVTSFITGIKLHDFNCGLKA
                     YRRELTKSIRMKRGMHRFVPALAHWQGFRVAEIPVQHHPRRFGKSKFGAKRFVDGFLD
                     LLTVVLLTRYVSRPLHFFGWVGTFLMVGGVAIEGYIAYLRLVHGWIMNRYPLLWLGVL
                     MIIVGLQMLSTGLLAELVANVKGDDGEVSILETIRAEGV"
     gene            complement(4510..5502)
                     /locus_tag="P5179_02905"
     CDS             complement(4510..5502)
                     /locus_tag="P5179_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS94201.1"
                     /translation="MSWWRSPWVSRVLKLLVSVVLIGGIVWQVGPDAVLPTSFSWWYG
                     IAAVAVFVLSNLLGAYQWNLLLKIAGIELPERVVTRAYFISLFFNNFLIGNVGGDVLR
                     AFEVRKNASPGLTHTTASGVATIVMDRFLGFFTMMIFAGAAALFTEEHSEFGALIAAV
                     LGSFVVLGVLITSRRIGTRVDACVVGLLPERVSRTITGLRAGFVAMRARWVVLGMAAA
                     VSFLVQGLRIYVHYLCALSIGVDIPLIYFWTFIPLIGVAAAIPISSGGLGPREWAAKW
                     LFTTLPGVGAGGVVAMELLAHVTALASSLPGAVAFVLRRESTRPPEPSQDLSTG"
     gene            complement(5499..6521)
                     /locus_tag="P5179_02910"
     CDS             complement(5499..6521)
                     /locus_tag="P5179_02910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS94202.1"
                     /translation="MSVVIPHYAGSQLFECLEALFSDTDAPGEVLVVDDASTDGTSEV
                     AQRRYPGIRLLRNKTNLGFVGACNRGLAEAAGAYAVLLNDDALPQPGWLQPLRERMER
                     DPQIAACQPKILRAHNSAIFDYAGAAGGLLDCYGYPFALGRWFDVCEADAGQYDAPRE
                     IMWASGAAMMLRMSVYRDIGGLEPVFQMHMEEIDWCWRARVAGYRIVSVPESRILHYG
                     AATLKAESLRKMYLNHRNSLLLLVKNYEARTLARVLPVRLLLELVTIFGGLVSLNWRR
                     ALGAAWGLVGAVAALPRILPARAQVQRLRRVRDHEIARYLYQGSIVLRWLLRQGAPAE
                     IPGAGA"
     gene            complement(6621..9962)
                     /locus_tag="P5179_02915"
     CDS             complement(6621..9962)
                     /locus_tag="P5179_02915"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS94203.1"
                     /translation="MSDKRVTRWLAFLMFLVVFGIYFRTMAPTTSFWDCGEFIACSFT
                     LGIPHPPGSPLFIMLGRVFSMFPIEGFLKAWGWLPEYADGAARVNIMSPLAGALSALF
                     CYLVILRLIRGWQGPLGTVKERWSDHVGAVAGTLIFALADSNWFNAVEGEVYAYAIFL
                     MMLALYLGLVWADSIGKPIHLPLALFLAYLMGLASGLHLLCLLVLPTIAFIGLFTYVK
                     DRYDAWKIVGAAALLVIGAYFAMHAMNARRLLGDEWAEPASLYGLSEDPMAYLQISIV
                     LAVIFGVAGVVVTFLTKTVKTRDIWAAIFVAGAAAATVQSLTGVFFWVENVQPFPTSR
                     VVGALGLLVILYFLSQSRERPSIPILKGYHLLVGMLLLVVVGYSTYLFLMIRSGLNPT
                     IDENNPENWKNLFDFLARKQYGNEDMSMIIFSRKASMQYQFWDMFIKYLLHQFPMSIS
                     GALFDWKLTFRSAMEVSYFGMRVPDFPLLLMFLGLGWHFNADRKRFFGMLALFIISGL
                     GLVIYLNMPDPQPRERHYVFTGAISVMAIWMGMGVTGLIRTAASWLPASVPEWLRTKF
                     LPVLMALIGFVVPVWFLLGYPLVDEYSGDFSVRYSNFVKHDRRYDTVGYDYAYNILQS
                     CDKDAVLFTNGDNDTFPLWYVQEVAGVRKDVRVVNLSLLNTDWYIQQLRDNEPKLPMS
                     ASWTNDWITNTLCGNTLESLISSGRIDLTPNGTPYDENGRVVGWVTKPVTAGRVDSAY
                     VELKNGEIRRGILGVPITGSTVLKEYGKAAWDTIPDAEVRVRLIGQSFPGITWDMPAS
                     PEYRVLRVQDVMIFKILDWVKWQRPVYFAVTVASDNLIGLDKYLRMEGMVLRITNQVD
                     STYMEGRGSYALDIERSKHNLDNVYQIRNMMDQRIYKDDNMLKLVSNYRSAYLRLAEK
                     QIDTGDTLGARETLVKFNQRLPRDWRGAYYGATVARRGGAQLDSLTQGYLREASQALL
                     NELPTMHVFDIYSLERVRMTAQLLRSTGQEQEAIRLLLATDPYLQKPSPLSGISSADR
                     IGLFFEASTIYDQVGDIQNAREMLERCRNLLMVIPNTAEANQEFARTFRTDAMTLAGE
                     VNRRIADLEQRARQTTPRSDSLPGR"
     gene            10276..10965
                     /gene="cysE"
                     /locus_tag="P5179_02920"
     CDS             10276..10965
                     /gene="cysE"
                     /locus_tag="P5179_02920"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRS94204.1"
                     /translation="MFRDVKAVFERDPAARGFWGVFEVVFTYAGLHALWNYRVAHFLH
                     RHGVPFIPRLLSQIGRFLTGIEIHPGAQIGRGFFIDHGMGVVIGETAIVGDDCTLYQG
                     VTLGGTGKETGKRHPTLGNGVVVGAGAKVLGNITIGNNVYIGANAVVLKDVPDDSTVV
                     GVPGRVVRQRGARVASALEHTGIGDPIMLKLDALQRDMVRFEQELRTLQNRNRDGVSE
                     NPEPEEPRPAQ"
     gene            11274..12563
                     /locus_tag="P5179_02925"
     CDS             11274..12563
                     /locus_tag="P5179_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS94205.1"
                     /translation="MGRHPNILQITTHDSGRHFGCYGHPTLHTPAIDALAAEGVRLTN
                     YFAAVPICSASRATQLTGLYPQTHGLMHLCGYPFRWRIRDDVRHMSQLLKAAGYTTHL
                     FGIQHEVVPDELPRLAFDSIRGQGFPSAKVIAADVADLLRSRGKEPAPFYAQVGFFQT
                     HTPFDHGGVKPDDERGVEVPPYLADCESSRKAMAAFQGAIRHVDEAVGFIVEALRKSG
                     LQDDTIVVFTTDHGIEMPRAKWFLYDPGVAIGCIVRYPGAGATGGTTCDLLLSNVDYL
                     PTLFDLAGIPVPGGLHGRSFAASLRGECSEPVREAIFALYHKSQSRCVRTSRYSLIRH
                     FDAPIDFATVPVCFEEVLAKRGIPQVELYDLEADPNQFENVASKPEYADIKVRLDTML
                     WEWMESVNDPLLKGPVRSPSYESAMADYGAWKGNAHT"
     gene            12625..13206
                     /locus_tag="P5179_02930"
     CDS             12625..13206
                     /locus_tag="P5179_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94206.1"
                     /translation="MPSSTLELDFKGPYRFVGRDRSVFEAPCAASPGVYLWTVKQHDG
                     THMIHYIGQTSAFAKRQREHLIQVLGLNYGIFNAEKAQTGVSELIWPGMCRKLPEEGP
                     LQAVEEYKRINEKVLEYLSVLNVFFAEADVGRSLLRHIEGCIGWNLRNNHPESKALFP
                     DGVRVGTLMQKDHGLLRIRSTEPIRGLDAEIPY"
     gene            13282..>14203
                     /locus_tag="P5179_02935"
     CDS             13282..>14203
                     /locus_tag="P5179_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94207.1"
                     /translation="MAHECGQKTINLPATTLPVTADADVVVVGGGTSGFIAAVAAART
                     GASVLLVEKAGYLGGCTTAPYNTGLSQFFDSDGNQIIRGLPWEFMTRMEADGNAFLVE
                     RRNEIWPVWTRKVALDMMEEAGAEFLFHTWTSGVVRDGNAVRGILVHNKGGTGVILGK
                     CFVDATGDADIAAFAGAPFDMTEVENLQQVSCDYIACGVDHLRVLEWARANKDRLGLH
                     VNNIDEPVKASGAQRMLSIVFPPNASDPATGEKYHVGVMPQVKLCIHRDAVRIQGNSD
                     INPLDPKALTRAEADGLRGALKHLQNLRDTI"
BASE COUNT         2880 a         4424 c         4218 g         2681 t
ORIGIN      
        1 atatttcttt gcgcacacaa cgcaggccat tccctttcag aagcggtcct ctcgatggac
       61 agtgctgctc acgggcgtac gtccggcgac ggacgcatgc catggtacta cggcgcgcgg
      121 ctgtatgtgt acatcctgcc gacgttcgtc ttcatcggaa tcttcgcgta ctacccctcg
      181 ctgagcgcaa tctatcattc gttcttcaac tggaacggtt cgaacataaa ggactacgtc
      241 ggccttgcga atttccgccg aatcttcctg aacgaccggg accttctggt tgcctcgtgg
      301 cacatgcttc tgttcgtctc ggcgaacctt gtcctgcaga ttccgtcaat tctgcttgcg
      361 gtagtcctgt accacctgcg gaacaaacgc gtgcagtact ggttccggat ggtattcgtg
      421 atcccgatgg tggtgcccgg catggtgggc atgctcctgt ggatgttcat ctataacccg
      481 caggtgggcc ttttgaaccg tgcgctggcc gggctcggaa tcattccttc gccattcgac
      541 ggtccgacct ggctggcgga cacgcgcctt gccatgccaa gtctcatgtt catggggttc
      601 ccgtatatca accccatcgc ccttctcatc tatctcgcgg ggttggaacg aatacccagg
      661 gacctgaacg attccgcgct gattgacggc gcaagcgcgt ggcagcggct tgtgcgggtc
      721 gaactgccga tgatcctcgg gcaggtgaaa ctgatgctga ttctcggcat tatcgggggc
      781 gtgcaggatt acggacgggt gctcatcatg acgggaggcg gcccctccga tgccaccacg
      841 gtcccggggc tcatcatgta caacaccgcc ttccggtacg cacagatggg ctatgcgtcg
      901 gccatcggcg tggtgatgtt tcttgtgata ctcggattga cggtgctcaa catgcggtat
      961 ttccgcacgc acgaggcgtc atgagaggta gacgcgtggt ctggaagtga acgtggcaaa
     1021 ggctcagtat ggcattgact agcaggcgtg cggaagccgc aaagttcaca ttgctggcag
     1081 gtgcggcgtt ttttgtgctt gtgccgttct gggtgatgat cagcatcagt ctgaaggacc
     1141 tgcgccagtt cctcgataac atcctttatc cgacttcccc gttccagtgg cagaattacg
     1201 tggaaggctg ggaggcggtg tccccgtata tcatcaacac tcttgtcgtc gcggcggtag
     1261 tgacggtggt aagtctcacg ttgagttcgc tgacggcgta tgtgttcgcg cggtatgagt
     1321 tccccggcaa aacgccgctg ttctatgcgt tgatcgtgct tctgatgatt cccggcattc
     1381 tcaacctcat tccccagtac gtgcttgcgc tgaggctcgg ccttgtaggc accgcgtggg
     1441 cgctgatcct gttctacgtc gcatcgcgcc aggtgcttga ggtgtacatc ctgcgcacat
     1501 tcttttcggg gctgccgaaa gagctgttcg aagcggcgca gatggacggg gcgagcgaca
     1561 tccggcaata ctggagcatc gcgttgccgc tttccaaatc cattctcggc acgctcgcca
     1621 tcatgacggt ggtgttcgtc tggaacgact acatctggcc actggtggct gctgataact
     1681 tcaaaaccgt ctccatcggg cttgcgtacc tgacaaagca gcactcaacc tcgtgggggc
     1741 cgctgatggc ggggtacgcc atcgcgtcac ttcccctgat cctcctcttc tcggtcacca
     1801 tgcgctattt tgtcgaaggc atcgccacgg gcgcgatcaa ggcgtaagcg tggcggtcgt
     1861 ctcgatacaa cgcggctatc gccacgccac tcgacgaccc gcgcgctaca ccctcacata
     1921 ccggaaaccc atcgcctcgg cgaaggcttc cacctccgcc gtgtgatcac cgatgccgag
     1981 tgcgatgtga tgagacgggc cctgctggca ccacgcatcc atgaactcgt gaatcggccg
     2041 ctccacccgc acgcggcagt tcgggttacc gattgagaga atgttccccg gcaccacttc
     2101 cccggctgtc gtgatgagtt tgaacgactc gccggcatcc acctgcgtca ggttcaccag
     2161 tgtcaccggc ccttccttca tgtggaagtc gatgccgagc ccgctgcccg atttcccgtg
     2221 gtgcacctcg aggaacgaaa gcttcgggcg gtcctgcgca acgttgatgt tgctcggacc
     2281 gtcgtgcccc atcaggatga agttctcgtc gaaatccatc gaaaagaact cgacgaacat
     2341 accaccggcg ccgagcagat cgagcagttt catggccatc gcggttttca cgtcgccctc
     2401 ggtgacgccg ggccttccca gcgcggcaag gcgcgacacg gcaagcgcgg cctgggcccg
     2461 aagttgcgtc accgcctcct tcattcccca ccagtagaac ccaaatgcgg acaggtcgtg
     2521 gtgcatgatt acttcgtcaa acgccaccgc gatgcgcgcc gattcgcgca ggtgttcatc
     2581 ggtgaccccc gcatccacgt cgtacatcgc gcggaactgc ccgcacatct cgtccacctg
     2641 cgcgcgggtc gcgtggtggt atgcctcctc gatctcctcc acttcggggc gaatcaggag
     2701 cctccccgtt gcattgagca gacgcagctc gtcgaccggc atatccgcca tattggtgta
     2761 ggtttcgccg atcaccccga agttgagggc cctgaacacc cgcgccgcag ccgcgccgcg
     2821 gcagtctgcg gcgacctcat cccagagccg cccgtcgcga aaggctcccg tgcgcacgcg
     2881 aaaggatttg ccgatcgtgc gcagcgccga ggcgtactcc ggcacgcagc agacgccctc
     2941 atggtgcagg tattccgtcg tatcggcttt ggcgtagtcg taggacgcgt cctcatgggc
     3001 gttgaggatg cggatcggca cgctcaggga gcgcgccgca ggaacaacgc acatggccgg
     3061 ggtgtagccg aatggaaaca ccagaacgat gtccacacgc tccttgagga agagttcgcc
     3121 cgcggcggcg gatttttccg gcgtgtccac cagctccggc gcgataacat ccccgaacgt
     3181 tccgagacgc tccagcagcc tgccgtacat ggcgaggcct ttggccctca gctcgggaaa
     3241 ttgctgccag tagtagtagt gcccggtcgg cagcaggccg attcgcggcc gcgccatgcg
     3301 cgcattcagg ctgaacatct gtcaactccg tgtgactgca gggttgtcgg acttgcgaat
     3361 ggctctcgtc atcggcttgg gggcgcgcgg gagggatatc acccggcaaa ggcgaacacg
     3421 cgacccaacg aaaacggaag gcgttgtcta ccctcgcggg gagactgccc ggtgacacaa
     3481 cggtgagcct tttgggaagc gcgtgggagg gcatgtctac gcaacgagag agggtcgcgc
     3541 atatcgggcg gtcgggctat actccctccg cccgaatggt ttcgagaatg gagacctctc
     3601 cgtcatcccc cttcacgttt gccaccagtt ccgccagcag acctgtggag agcatctgca
     3661 ggccgacgat aatcatcagc acgccgagcc acaggagagg atagcggttc atgatccatc
     3721 cgtgcaccag ccgcaggtac gcgatgtatc cctcaatcgc cacgccacct accatgagaa
     3781 acgttcctac ccagccgaag aaatggaggg ggcggctgac atagcgcgtc aacagcacca
     3841 cggtgagcag gtcgaggaat ccgtccacga agcgtttcgc gccgaatttg ctctttccga
     3901 acctgcgggg gtggtgctga accggtatct ctgccacgcg aaacccctgc cagtgcgcga
     3961 gagccggaac gaaccggtgc attccccgct tcatgcgaat gcttttcgtc aattcgcggc
     4021 ggtaggcctt caggccgcag ttgaagtcgt gcagcttgat accggtgatg aaagacgtca
     4081 ccccgttgaa aaccttggac ggcagggttt ttgaaatcgg gtcgtggcgg atcaccttcc
     4141 agccggtaac gagatcgtat ccctcgtcca gcttcgcgat gagattcgga atctcctccg
     4201 gatcgtcctg gagatcagcg tccatcgtaa ccacgatatc gcccgtggca acccgaaacg
     4261 ccgttgccag cccggctgat tttccgaagt tgcgccggaa ccgcaccgcc ttgacacgtg
     4321 gatccttctc cgcaagttca cggataaccg agaaggaacc gtcacggctc ccgtcgtcaa
     4381 caaacacgat ctccgcgaca tatccctcgc gatccaccac cctggtgatt gcttcgtgaa
     4441 gcggacggag gctttcttcc tcgtccagca aggggatcac gatcgagact gtccgtggag
     4501 acatgagtgt catcctgtgg agaggtcctg tgaaggctca ggcgggcgcg tggattccct
     4561 ccggaggaca aacgccacgg cgccgggaag gcttgaggca agtgccgtca cgtgtgccag
     4621 aagctccatg gctaccactc cccccgcgcc gaccccgggc agcgtcgtga aaagccattt
     4681 ggctgcccat tcccggggcc cgagtccacc cgaacttatc ggaatcgccg cggcaacccc
     4741 gataagcggg atgaacgtcc agaagtaaat taacgggata tccaccccga tggacaacgc
     4801 gcacaggtaa tgcacgtaaa tccgcaaccc ctgaacaaga aacgaaacgg ctgccgccat
     4861 tcccagcacg acccagcggg cccgcatcgc cacgaagccg gcgcgaagcc cggtaatcgt
     4921 tcgcgacacg cgttcgggca gcaggcccac cacgcacgca tctacgcgcg tcccaatgcg
     4981 ccggctcgtg atcaggacgc ccagcaccac gaaactgccg agaaccgccg cgatcagagc
     5041 gccaaactcc gaatgctctt ccgtgaaaag agctgcggcg cccgcaaaga tcatcatggt
     5101 gaagaacccg aggaagcgat ccatcacgat ggtggcgaca cccgaagcag tggtgtgggt
     5161 cagtccggga gacgcgtttt tccggacttc gaacgcgcgc agtacgtcac cacccacgtt
     5221 gccgatgagg aagttattga aaaacaggga gatgaaatag gcgcgcgtga caacgcgttc
     5281 ggggagctca attcccgcga ttttcagaag caggttccac tggtaggccc caagcaggtt
     5341 gctgaggaca aacaccgcga ccgccgcgat accgtaccac catgagaagg aagtcggcaa
     5401 aacggcatcc ggtccgacct gccagacaat ccccccgatc agcacgacgc tgaccagaag
     5461 cttgagaacg cggctgaccc acggagagcg ccaccagctc atgcgccggc tcccggaatc
     5521 tccgcgggag caccctggcg aaggagccag cgaaggacga tactgccctg atacagatag
     5581 cgagcgattt cgtggtcgcg gacgcgcctg agcctctgca cctgtgcccg tgcgggcaga
     5641 atccggggta atgccgccac cgcgccgacc agcccccacg ccgcgcccag tgcacgccgc
     5701 cagttcagtg agacgagtcc cccgaaaatg gtcaccagct caagcagaag gcggacaggc
     5761 agcacgcgcg caagcgttcg cgcctcgtag ttcttcacga gcaataggag gctgttccgg
     5821 tggttaagat acatcttgcg aagggattcc gctttcaacg ttgcggcgcc gtaatgaagg
     5881 atcctcgatt ccggcacgct gacaatgcga tacccggcca cccttgcgcg ccagcaccag
     5941 tcgatttctt ccatgtgcat ctggaacacc ggctccagcc cgccgatatc acggtacacg
     6001 ctcatgcgca gcatcatcgc cgcgccggat gcccacatga tctcccgcgg ggcgtcgtac
     6061 tgaccggcgt ccgcttcgca cacgtcaaac cagcggccca gcgcgaaggg gtacccgtag
     6121 cagtcgagga gaccacccgc cgcgccagca tagtcgaaaa tcgcggagtt gtgcgcgcgg
     6181 aggatttttg gctgacacgc ggcgatctgc gggtcacgtt ccatgcgttc gcgcagcggt
     6241 tgcaaccagc ccggttgcgg gagagcgtcg tcattcaaca aaacggcgta tgcgcccgcc
     6301 gcttccgcca gcccgcgatt gcacgcgccc acaaaaccga ggtttgtctt gtttcgaagt
     6361 agccgaatgc cgggatagcg ccgctgtgcc acctccgagg tgccgtcggt ggacgcgtca
     6421 tccaccacca gcacctctcc cggtgcgtcc gtatctgaaa acagcgcctc aaggcattca
     6481 aaaagctgcg atcccgcata gtgcggaatc acgacggaca cgtcatcccg tgcgatcgtg
     6541 ctccctttct tcgaaaggcc gcctttttgt gaagggcagc ctttcccttg aaaccggaat
     6601 gacccttttc ccgcgctctt tcaccgaccc ggcagcgaat cgcttcgagg tgtggtctgg
     6661 cgtgctctct gttccagatc cgcgatgcgg cggttcactt cgccggccag cgtcatggcg
     6721 tccgttcgga acgtccgcgc aaattcctgg ttcgcttcgg cggtattggg aatcaccatc
     6781 agcaggttcc ggcagcgctc gagcatttcc cgcgcattct gaatgtcccc gacctggtca
     6841 taaatcgtcg aagcctcgaa aaacaggccg atacggtccg cgcttgagat gccggaaagc
     6901 ggggacggtt tctgcagata gggatccgtg gccaacagta gcctgatcgc ctcctgctcc
     6961 tggccggtgg aacggagcaa ctgcgccgtc atgcgaacac gttccagcga gtagatgtcg
     7021 aacacgtgca tggtgggcag ttcgttcagc aacgcctggg aagcttcccg cagatacccc
     7081 tgtgtcaggc tgtcaagctg tgcgccgccc cgacgggcaa ccgtggcgcc gtagtacgca
     7141 ccgcgccaat cgcgcgggag gcgctggttg aacttcacga gcgtctcccg ggcacccagt
     7201 gtgtcacccg tatcgatctg cttctccgcc agccgcaggt aggcggagcg gtagtttgag
     7261 accagcttca gcatgttgtc gtccttgtaa atccgctggt ccatcatgtt ccggatctgg
     7321 tatacgttgt ccagattgtg ctttgagcgc tcgatgtcga gtgcgtacga gccacgccct
     7381 tccatgtagg tgctgtccac ctggttggtg attcgaagaa ccatgccttc catccgcagg
     7441 tacttgtcga ggccgatcag gttatcggac gccacggtga ctgcgaagta gacgggccgt
     7501 tgccacttca cccagtcgag gatcttgaaa atcatcacgt cctgcacacg aagcacgcgg
     7561 tactcaggcg aagccggcat atcccacgtg attccgggga aggactgacc aatcaaccga
     7621 acacgcacct ccgcatccgg aatcgtatcc cacgcggcct tgccgtactc cttcagcacc
     7681 gtgctgccgg taatcggaac cccgaggatg ccgcgccgga tctcgccgtt tttcagctcg
     7741 acgtacgcag aatccaccct tcccgccgtc acgggttttg tcacccagcc caccacgcgg
     7801 ccattctcgt cgtacggcgt gccattcgga gtgagatcaa tccggcccga actgataagc
     7861 gattcaagcg tgttgccgca cagggtgttg gtgatccagt cgttcgtcca gctcgcgctc
     7921 atgggaagtt tcggctcgtt atcccgcagc tgctggatgt accagtcggt gttcaaaagg
     7981 ctcaggttga ccacccgcac atccttccgc acgccggcca cttcctgaac gtaccagagc
     8041 gggaaggtgt cattatcccc gttggtgaac agcacggcgt ccttatcaca cgactggagg
     8101 atgttgtacg cgtagtcgta accgaccgtg tcatacctgc ggtcgtgttt gacgaagttg
     8161 gaataacgca cgctgaaatc gccggaatac tcgtccacga gcgggtagcc cagcaggaac
     8221 cagaccggca cgacaaaccc gataagggcc atcaggacgg ggaggaattt cgtccgaagc
     8281 cattcaggca cggacgcagg cagccacgac gccgccgtcc gaatgaggcc ggtcaccccc
     8341 atgcccatcc agatcgccat caccgaaatc gcgccggtga acacgtaatg ccgctcacgc
     8401 ggttgcggat cgggcatgtt cagatagatc accagcccca gaccagagat gatgaacagg
     8461 gcgagcatgc cgaaaaagcg ctttctatca gcattgaagt gccacccgag gccaaggaac
     8521 atgagcagga gcgggaaatc gggcacacgc atcccgaagt aagaaacctc cattgccgac
     8581 ctgaacgtga gcttccagtc gaacagagct ccggagatgc tcatggggaa ctggtgcagc
     8641 aggtatttga tgaacatgtc ccagaactga tactgcatgc tggctttgcg gctgaagatg
     8701 atcatggaca tgtcttcatt gccgtactgc ttgcgggcaa ggaagtcaaa gaggttcttc
     8761 cagttctccg ggttgttttc gtcaatcgtg gggttcaatc ccgaccggat catgaggaag
     8821 aggtacgtgg aatatccgac caccaccaga agcaacattc cgaccagaag gtgatacccc
     8881 ttcaggatgg gaatggaggg tcgctcccgg ctttgcgaga ggaagtacag gatcaccagc
     8941 aacccgagag cgccaaccac gcgcgaggtc gggaagggtt gcacattttc aacccagaag
     9001 aacactcccg taagcgactg aaccgttgcc gccgcggctc cggcaacgaa tatcgccgcc
     9061 cagatgtcgc gagttttcac cgtcttcgta aggaacgtca ccaccacgcc ggccacgccg
     9121 aaaatcactg ccagcacgat gctgatctgc aggtaggcca tggggtcttc gctgaggccg
     9181 tacagcgacg ccggttccgc ccattcgtcg ccgagcagac ggcgcgcatt catcgcatgc
     9241 atcgcgaagt acgcgccgat aaccagcagg gccgcggcgc cgacgatctt ccacgcgtca
     9301 taccggtcct tgacatacgt gaaaaggccg atgaacgcga tggtggggag cacaagcaga
     9361 cacaacaggt ggagcccgct ggccagtccc atgagatacg ccaggaacag ggcaagcggc
     9421 aggtgaatcg gttttccaat cgaatcggcc cacacgaggc cgagatacag cgccagcatc
     9481 atgaggaaga tggcgtacgc gtacacctcg ccctcaactg cgttgaacca gttggaatcc
     9541 gccagcgcga aaatcagtgt gcctgccacc gcgccgacat ggtccgacca gcgttccttc
     9601 acggttccga gagggccctg ccagccgcgg atcagacgca gaatgacgag gtaacagaac
     9661 aacgccgaaa gagcgccggc taacggggac atgatgttca ctcgcgcggc accgtcggcg
     9721 tattccggaa gccatcccca cgccttcagg aacccctcga tcgggaacat cgagaacacg
     9781 cggcccagca tgatgaacag cggggacccc ggtggatgag ggattcccag cgtgaacgag
     9841 catgcgatga actccccgca atcccagaag gatgtcgtcg gtgccatagt ccggaaatag
     9901 atcccgaaca cgacaaggaa catcaggaac gcgagccaac gggttacccg tttgtcactc
     9961 atgccacaat ccctctggtt gaatcacgcg gaaatcgtga tgccgcccgc ccgaaatcgg
    10021 aacttcctcg ccgcctcggg gggtaatggt ggtagagatt agagcagatt gcatccgctc
    10081 aaagactcgt tgagaaatgt gagaataccg cccgtgcgcg gccaagtcaa gcgttgcacc
    10141 cggcggagcc acgccgtggc ccgcgagagc gatccggccg attgacatcg cccgcacgcg
    10201 ccgagtattc ttctagtccg actgcagacg atgcgccagg gcgcccggcg cgccgggcaa
    10261 aaggagccag aacgtatgtt tcgcgacgtc aaggcggttt ttgaacgcga tcccgcagcc
    10321 cgtggcttct ggggcgtctt cgaggtggtt ttcacctacg ccgggctcca cgctctgtgg
    10381 aactatcgcg tggcgcattt tctccacagg catggcgtcc cgttcattcc ccggttgctc
    10441 tcccagatcg ggcgcttttt gaccggcatc gagatccatc cgggcgcaca gattggccgc
    10501 gggttcttca ttgaccacgg aatgggtgtc gttattggcg agacggcgat tgtcggggac
    10561 gattgcacgc tgtatcaagg cgttacgctg gggggaacgg gaaaagaaac cggcaagcgc
    10621 cacccgacgc tgggaaatgg cgttgtggtg ggcgcgggcg cgaaggttct cggcaacatc
    10681 acgattggca acaacgtcta catcggtgcc aacgccgtcg tcctcaaaga cgttcctgat
    10741 gacagcaccg tggtgggcgt tcccggccgc gtggtgaggc agcgcggcgc acgcgtcgct
    10801 tccgcgcttg agcacaccgg aatcggcgac cctatcatgc tgaagctcga cgccctccag
    10861 cgcgacatgg ttcgcttcga acaggagttg cgcacccttc aaaatcgcaa ccgcgacggt
    10921 gtttcggaaa acccggaacc ggaagaaccc cgcccggcgc aataatgccg aagaccctcc
    10981 ccgcacctcc tccaccgttc aatgtggcgg caggaaggta cgagtcggca gccatcgtct
    11041 ccctgtctca agttcctatc ctcaatatcc tcaacttgtt gtcgcacccc agagcccgca
    11101 gcccgcgtac cccgattgaa cttttttcac agaaacactt gacacgaaca ccccgcgtcg
    11161 ccacagcttg atcgaggtca cccggcggta gggtaccggc ggaagggcgt acgcgggggt
    11221 tgggctgggc atccgtaatc gccattttgc acagcagggg aaaggagcaa gccatgggca
    11281 gacaccccaa cattctgcag atcaccacgc acgattccgg gcgccatttc ggctgctacg
    11341 ggcatccgac gctccacacc cccgccatcg atgcgctggc cgccgagggt gttcgcctca
    11401 ccaactactt cgccgccgtg ccgatctgca gcgcgagtcg ggccacgcag cttaccggtc
    11461 tttacccgca gacgcacggc ctcatgcacc tctgcgggta ccccttccgc tggcgcatcc
    11521 gagatgacgt gcgtcacatg agccagttgc tcaaggcggc aggctacaca acgcacctgt
    11581 tcggcatcca gcatgaagtc gttccggacg aactgccccg tctggcgttc gactccatcc
    11641 gggggcaagg tttcccgtcc gcgaaggtaa ttgccgccga cgtggcggat ttactgcgtt
    11701 ctcgaggaaa ggaaccggcg ccgttctatg cgcaggtcgg gtttttccag acccacaccc
    11761 cgtttgatca cggcggggtg aagcctgatg acgagcgcgg ggtggaagta ccaccgtatc
    11821 ttgccgattg tgaatcgtcg aggaaggcca tggcggcctt tcagggcgca atccgccacg
    11881 tggatgaggc ggtcggcttc atcgtggaag cgttgcgcaa gagcggcctc caagacgaca
    11941 ccattgtggt cttcaccacc gaccacggga tcgagatgcc gcgcgccaag tggtttctct
    12001 acgaccccgg cgtcgccatc gggtgcatcg tgcggtaccc gggggccggc gcgaccggcg
    12061 gcacaacgtg tgatctactc ctcagcaatg tggattacct ccccacactg ttcgatctgg
    12121 cgggaatacc ggttccgggc ggcctccacg gcaggagttt cgccgccagt ttgcgaggcg
    12181 aatgctccga acccgtgcgc gaggccatat tcgccctcta ccacaagtcg cagtcccggt
    12241 gtgtgcggac cagtcgttac tcgctgatcc gccatttcga cgccccgatc gatttcgcca
    12301 ccgtgccggt gtgtttcgaa gaggtgctgg cgaagcgggg cataccacag gtggagctgt
    12361 acgatctgga ggccgatccg aaccagtttg aaaacgtcgc ttcaaagccc gaatacgcgg
    12421 atatcaaggt gcgcctcgac accatgctgt gggagtggat ggagagcgtg aacgacccgc
    12481 tcctgaaagg gcctgtgcgt tccccgtcat atgagtccgc gatggcggac tatggcgctt
    12541 ggaagggcaa cgcgcacacc tgatggcggg gcggcttttg gaggggcgcg ttccgtcgcg
    12601 cccgtcggga agggaaccac ataaatgccg tcatctacgt tagaactgga cttcaagggc
    12661 ccctatcgct tcgtcggtcg ggatcggtcg gttttcgagg ctccttgcgc cgcgtctccc
    12721 ggcgtgtacc tgtggaccgt caaacagcac gacggcacgc acatgatcca ttacatcggc
    12781 cagacgagcg cgttcgcaaa acgccagcgt gagcatctca ttcaggtcct ggggctcaat
    12841 tacggcatat tcaacgccga aaaggcgcaa acgggtgtga gcgaactcat ctggcctgga
    12901 atgtgtagga agctgcccga agaagggccc ttgcaggctg tggaggagta caagaggatc
    12961 aacgaaaaag tactcgagta cctctccgtc ctcaacgttt tttttgcgga agcggatgtt
    13021 ggacggtcgc tgctcaggca cattgagggt tgcatagggt ggaaccttcg aaacaaccac
    13081 ccggagtcga aggctctgtt tccggatggt gtccgggtag gaacgttgat gcaaaaagat
    13141 cacggtctgc tgcgcattcg ctccacggaa ccgattcggg ggctggatgc ggagattcct
    13201 tattgacttg aagcctccat gagcggcgcc tgtgcgtttc agacaagcgg aatcgggggt
    13261 tcaccggcaa aggagaggac catggcccat gaatgcggtc aaaagaccat caacctcccc
    13321 gccacaaccc ttcccgtcac ggcggacgcc gacgtggttg tggtgggcgg tggaacatcc
    13381 ggcttcatcg ccgccgtggc cgcggcacgg acaggagcca gtgtcctgct ggtggagaaa
    13441 gccgggtacc tcggcgggtg cacaaccgcc ccgtacaaca ccgggcttag tcagttcttc
    13501 gactcggacg gcaaccagat tatccgcggg cttccgtggg agttcatgac gcgcatggag
    13561 gccgatggga acgccttcct cgtggagaga cgcaatgaaa tctggccggt gtggacgcgg
    13621 aaggtcgcgc tcgacatgat ggaagaggcg ggcgcggaat tcctgttcca cacgtggacg
    13681 tccggcgtgg taagagacgg caatgcggta cgcggcatcc tcgtgcacaa caagggcgga
    13741 accggggtga tcctcggcaa atgtttcgtg gacgcaaccg gcgacgccga tatcgccgcg
    13801 ttcgccggcg cgcccttcga catgacggag gtggagaacc tgcagcaggt gtcgtgcgac
    13861 tacatcgcct gcggggtgga tcacctccga gtgctcgagt gggcgcgcgc caacaaggat
    13921 cgcctcggtc tgcatgtgaa caacattgac gagccagtga aggcgtccgg cgcccagcgc
    13981 atgctctcca tcgtcttccc cccaaatgcg agcgacccgg ctaccggcga gaaataccat
    14041 gtcggcgtga tgccgcaggt gaagctctgc atccaccggg atgccgtgcg gattcagggg
    14101 aactcggaca tcaacccgct cgacccgaaa gccctcacgc gcgccgaagc ggacggcctg
    14161 cgcggcgcgc tcaagcatct gcagaatctg cgcgacacga tcc
//
LOCUS       DAOTNG010000036        14171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4204_length_14171_cov_4.967555, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000036 DAOTNG010000000
VERSION     DAOTNG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_14171_cov_4.967555"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="P5179_02940"
     CDS             complement(<1..849)
                     /locus_tag="P5179_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRS94208.1"
                     /translation="MTSGIFPAFLALVAAQVASASISVRVTNTAGEAVPSALVTVVGA
                     RAACFTDSLGTCSLAEVFPTGCLIAASAPGFLPDTLPLPATHHIAFVLRTNPIVLEPL
                     AVTADRLRDSERSTAFVTSIETEELQVRAETVPELLNDIVGVQVRSTGGFGSFSTVSI
                     RSSSSEQVRVYLDGIPLNQALGGGVNLAAIPISVVERVDVYRGVIPPIFGGSGTAGVV
                     NFITRQATDSLRWQANASYGTWDTRTASGWVTRRIGPVAGIASVDFSRSDNDFAYRDD
                     NGTTLNT"
     gene            843..980
                     /locus_tag="P5179_02945"
     CDS             843..980
                     /locus_tag="P5179_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94209.1"
                     /translation="MSRALSDGAGEPQAGRGSKGRKDELSPLNPPAQVRSRVTQFRQV
                     S"
     regulatory      complement(949..1147)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1282..2727)
                     /locus_tag="P5179_02950"
     CDS             complement(1282..2727)
                     /locus_tag="P5179_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS94210.1"
                     /translation="MTLRGHEERPNVVVILSDDQGPWAMRCTGNSELETPNLDRLAAT
                     GMRFDRFFCTSPVCSPARASLLTGRIPSCHGVHDWLAAGNTIAKYEPARKGEFIDFLV
                     GQPAYTDELAKAGYACGLSGKWHLGDCHRPRDGFVFWDAHAKGGGPYYRAPMVRHGEV
                     YEEPNYVTDAITMNALRFLEQRKSHSQPFYLSVHYTSPHSPWDRENHPKELFDQYHDG
                     CPFTSTPDLMPAPDWVKHRSIPVDDAGSRRQKLSGYYAAITAMDTNIGKIIDWLEENG
                     KRENTLVVFTSDNGMCMGHHGLWGKGNASFPLNMFEESVRVPLLASHPGVIEGGALNS
                     DLLSQYDFSSTLLEYVGISTPSDPISPGRSFCELLKGGRTDPHEHLVVFDEYGPVRMV
                     RTREWKYVHRYPYGPNELYHLINDPAETENRISDPAARPVLREMRERLHEWFAIYSDP
                     DCNGATEPVTGRGQTGLCGSRARGEDVFIPF"
     gene            complement(2792..3868)
                     /locus_tag="P5179_02955"
     CDS             complement(2792..3868)
                     /locus_tag="P5179_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRS94211.1"
                     /translation="MANLWGKHWTKRELLERVGDISQIADVRKSVLADGNNTGVEVFD
                     FATGSGFAFTVAAGRAMDVVAASYQGKALAWRSSVGDPHGSFYDPEGLEWLRQFFGGL
                     VATCGLSYAGAACTDEGKKLGIHGRIGNVPAKRANFGGYWEGDEYILWASGVMRESGL
                     FQDELELQRVISTKLGANSFTIRDKVVNIGWHTTPLMILYHCNYGFPVVANGSEVLTP
                     AVKVTPRDADAREGAETYFRLHEPVKDYREKCYYHDVNAGPDGRTAIGIVNREMGIGG
                     YIRYRPEQLPKLIQWKQMGQGWYTCGLEPANCLVEGRDKDRATGRLQFIEPQEERIFE
                     LELGVLTNQEEIAQFEAEVNRIRG"
     gene            complement(3982..5073)
                     /locus_tag="P5179_02960"
     CDS             complement(3982..5073)
                     /locus_tag="P5179_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRS94212.1"
                     /translation="MNIRSVSSYVVHGKCFVVVQTEEGVEGVGECSPMHSETTARFVA
                     EVLGPRIIGKNALETDKLWHVMMLDTYKLGVQGIQPEAVAGVDIALWDIRGKATGQPL
                     CMMLGGCYREHIPMYASIGGGATMTPREMATRVEKAVHLGYKGVKIRMDWGVARQDRG
                     PEKDLRMVEECKQVCGTAVPLSFDANNGYSVPTAIRQGRKFEALGLRHFEEPVAQLDY
                     EGLGKVADALDLPVSAGEHEYTRWQFRDLILNARVDIVQPDVVKCAGITETMKIATLA
                     EVFNRLFLPHQTQPSIGTAVNVHICAALHWCDLPQEYTGQSPELDELFEEPLVFYDGA
                     ITVPLRPGHGLVLKKSALDGATRFSTSAN"
     gene            complement(5099..5773)
                     /locus_tag="P5179_02965"
     CDS             complement(5099..5773)
                     /locus_tag="P5179_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HRS94213.1"
                     /translation="MGLLTPRGQTLFHETFDEGLTQWRLEGIGSPRVVENARGERALR
                     LDSVGSIQGAEGCNFFHVKDFPDNIVLDFDLYVHFSDGLFITFVAVRGLNGEDMFSLP
                     PRSGVFGDYVAKDALLRSYHVSVSRYNDKGEHTGVSNWRRNPGLHLMAQGPDPCRQIK
                     TFYHLTVVKAGPHLQLGVNGVLAHEFVDPQTLPIPLPDSGKIGFRAIGSQIVVDVSNI
                     RVTSLD"
     gene            6059..7219
                     /locus_tag="P5179_02970"
     CDS             6059..7219
                     /locus_tag="P5179_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94214.1"
                     /translation="MEKTIGVGMIGYAFMGRAHSNGWRQVGHFFDLPMKPVMKTICGR
                     NKAAVTAAAKTMGWEKAETDWMKMIKDPQIQIVDICTPGNEHKPMALAALKAGKHVVC
                     EKPLANTLAEAKEMAHAAKEAGVCTLTAFNYRRVPAIALAKQMIDQGLLGTIYHWRAV
                     YLQDWIIDPKFPLVWRLRKEVAGSGPHGDLNAHITDLARYLVGEITEVVGMGKTFIKT
                     RPIQEALADGGLGAKAGTKKGKVTVEDAMLFLAKFENGALGSFEATRFAAGRKNFNSF
                     EINGSKGSIAFNLERLNELQYFNRKDDANLQGFRTILCNEGGQPYNAAWWPSGHIIGW
                     EHTFTHEFKDFIEAIAAKKDTMPNFEDGMKTQAVLEAVMASADKKKWVKVPL"
     gene            7334..7810
                     /locus_tag="P5179_02975"
     CDS             7334..7810
                     /locus_tag="P5179_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HRS94215.1"
                     /translation="MILEVKHRPGKPPLEWACEWVDGEVGKWARVRYVSDTEQKVQDT
                     VLPVGTVTDGVYWADRPFHVWKFSTGAGGHIGYRFDVCTNTFIWKEKLIWYDLELDLW
                     IPWGGSPQWQDEDDMRRLVRMEHLSQEELEVAEQAKKYLDNHWRTVIECAFGEVHS"
     gene            7903..8925
                     /locus_tag="P5179_02980"
     CDS             7903..8925
                     /locus_tag="P5179_02980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS94216.1"
                     /translation="MALSDERFLRSRFDVRAFRIILAPPVRVLTNTATLAVWLCWNLP
                     RARGVFFRFADYYAFLPVFLSRLFRRKSWIVLGGYDAHHFPEYGYGVYEQPLRASIVR
                     YAVRNATAVLPVDQSLYDGVNAYAFDPPRPTGIKSLVPNFRSEVRVVADGFDHEFWTP
                     SPGCRKERLVVATASVPQSTSRESRMRMVVLKGVPLLLEVARRMPDVTFAIVGPEGGV
                     LPQGGQAVPANVQLPGYLSPEELREYYRRAAVYAHPSLTEGLPAAVAEAMLCCCVPVG
                     SSVNGIPELIGDTGFVLTRPAVDEWVAAVTRALELGASSRARDRIVALFSLAKRHRML
                     LDVIEG"
     gene            8998..9360
                     /locus_tag="P5179_02985"
     CDS             8998..9360
                     /locus_tag="P5179_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRS94217.1"
                     /translation="MPSIAIIGASKDRSKFGNKAVRAYVRQGYTVYPVNPKEREIEGI
                     LAYPSVKSIPFPVDRVALYVPPQIGIALLPEIAGKHPAELFVNPGAESEELLDEARRL
                     GLEPIVACAIVSVGEMPD"
     gene            9376..9831
                     /locus_tag="P5179_02990"
     CDS             9376..9831
                     /locus_tag="P5179_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRS94218.1"
                     /translation="MRKVVKARPRLENSPSDGAKALFLEAVRALRPEDIPVKPVDRGE
                     GDASEPVARRRTERVVESIDLHGMTAEQAIRYLTEVLPDYRRTGGTVQIVVGKGLHSP
                     DGQGVLREVVSGWLDHAGKRFVSRWRWGGRRQGGNGVIVAAIRVPGKPD"
     gene            9847..10977
                     /locus_tag="P5179_02995"
     CDS             9847..10977
                     /locus_tag="P5179_02995"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94219.1"
                     /translation="MNRNLLRELIEPILNTPTGPFYEDHVASFVAETVRGTGLTMSTD
                     TFGNILVRRPSVGRNNLRIGFMAHMDHPAFEVIEQLEGAVRVAWHGGVDAEYFPQARV
                     RFHTPGNPCTGEIREVELEEARNRVKTAVVACDSPPSVGTLATWELPPVEVTNGVLRA
                     PAVDDVAGCGLLLALLLELGDRPLETEVWTLFTRAEEVGFVGAIGLARSGRLPVSTPV
                     VSVEMSKATAGVTQGMGPVIRLGDRSSIFDAMFVLFMKDVASDLKGRIEGFKYQQAMM
                     EGGSCEATALNLFGYRTAGLAVPLTNYHNQRPSGPSGPFVIDSEEIAINDFANAVDLC
                     VGMCERFRGLDAIRSGHRSKAVERAAEGLQRLERMERARTRR"
     gene            complement(11023..12096)
                     /locus_tag="P5179_03000"
     CDS             complement(11023..12096)
                     /locus_tag="P5179_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS94220.1"
                     /translation="MTYGVIMAGGKGTRLWPEGRKTRPKQLLDLIDHRPMIRVAVDRL
                     APLIPVERQIIVTTREYASEIARALPAFPAENILVEPLGKDTAPCIGWAALKVTERDP
                     AATMAVVTADHFIADETGFREVLHAAVTVAENAPCVVVVGFVPLRPDTGLGYIRFGHE
                     TREIEGIQFYRVDEFVEKPNKAKAIQYVEDGRYLWNSGMFVMQARHALNLFSRFLPRH
                     FALLEEIKSSLGTPDEPAVTENAYQRFDRVSLDVGIMEKADNVEVIRADFGWLDIGTW
                     AALDQISLHDARGNVVRGQHVGLDTERCIIRTSNSVVATMGVHDLVIIETDAAVLVCP
                     KSRVQEVKNLVEALEREGREDVT"
     gene            complement(12113..13588)
                     /locus_tag="P5179_03005"
     CDS             complement(12113..13588)
                     /locus_tag="P5179_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS94221.1"
                     /translation="MVRIHHLVAALAFTVFMSSCLPVRQTEPTPRPVALHPDDLPPFD
                     SLKFTPPPVFRTTLPNGITLFVLTDNEQPIVNVDILIRGGSQCDPAGKPGVASFTAGV
                     MRRGGTLHLSADALDDTLDYLAAHLNVGMDVDCASANLNVLRKDFDSGLAMLADVLIS
                     PAFPENKIVELQRLTKESIRRRNDDPYEIGRRRFRQIVYGQGNPWARQLELEDVDRIT
                     REDLVAFHRTWFRPNVTYMAISGDITPEEAVAKVSRAFAGWAVAEVPPLPSPEQNHGM
                     PPGVYHIQKAVNQSAIRMGSTGLRRHDPDQYAVAVMNQIFGAGTFTSRLGQEVRSNRG
                     LAYYVFGIVLDSPDPDQGMFLAAVGTRADKTSETISVMRSVIDSMSIKPVTDDEISAA
                     KETIINSFVFSYASPERIAGQEMRLVFDGYPEDYLTSYIPRIRSVTKDDVVRVARKYL
                     RSDQLRVLVVGDSTRFDGDLGALGPLTSLPLDDPSGDQAPR"
     gene            complement(13591..>14171)
                     /locus_tag="P5179_03010"
     CDS             complement(13591..>14171)
                     /locus_tag="P5179_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS94222.1"
                     /translation="VDFDAEPQLMVGYHKPNYPDPDAYVFSVIANILRDSGNSSRLYR
                     RLVQTGIASDVAVYEELPGQRYPNLFVLHASPAAPHTTGEVEEILYSELDSLARVPVS
                     EAELTKCKNQLEASYIREISDNNSLAQALARAQAIHGDWRVVTEHRANVSKVTADDVM
                     RVARLYFKPQNRTVAAIVRENEQNNGRGREDQ"
BASE COUNT         2925 a         4030 c         4160 g         3056 t
ORIGIN      
        1 cgtattgaga gtcgttccgt tatcgtctcg gtacgcaaaa tcgttgtcgc tccgggaaaa
       61 gtccactgag gcaataccgg caacagggcc tatgcgccgc gtgacccagc cgctggcagt
      121 tcgcgtatcc catgtgccgt aagatgcgtt ggcttgccag cgcagggagt ccgttgcttg
      181 ccgggttatg aaattgacca cccccgccgt tcccgaaccg ccgaagatgg gtggaatgac
      241 tccccggtac acgtcgacgc gttcgacaac ggaaatcgga atggccgcca ggttaacccc
      301 tccaccaaga gcctggttca gcggaatgcc gtcgaggtac acacgcacct gttcggatga
      361 gctgctccga atcgagaccg tgctgaagct gccaaagcct ccggtggaac gaacctgaac
      421 tccgacgatg tcgttaagaa gctccggtac cgtctcggcg cggacctgga gctcctccgt
      481 ctcaatcgag gttacaaacg cggtcgatcg ctccgaatcc cgcaggcggt cagcggttac
      541 cgccaacggc tccagtacga tggggtttgt gcgcagaaca aacgcaatat gatgcgtggc
      601 cggcagcggt aaggtatccg ggaggaaccc gggagcagac gccgcgataa ggcagccggt
      661 tggaaagacc tctgcaagcg agcacgtgcc gaggctgtcc gtgaagcagg cggcgcgtgc
      721 ccctacgact gtcacgagcg ccgaaggaac ggcttcaccg gcggtgtttg tcacccggac
      781 cgagatcgag gcagatgcga cttgagccgc cacaagggcc aaaaatgcgg ggaaaatgcc
      841 cgatgtcacg cgcgctctcc gacggagcag gcgaaccgca ggcaggacgg ggatcgaagg
      901 gcaggaaaga cgagctttcc ccgctcaacc cgcccgcgca ggttcgcagc agagtgacgc
      961 agttcaggca ggtctcctga ctcgcggcag caatcgtccg ccttcccagg ttccgagcac
     1021 ccagtggcat tacatgacga gtgccattgc accgcttaca gtggcggggc cgtaccggat
     1081 tttcaccggt ttccctattc tccgaggcgt gttcccacgc gccggcacct gagctgaggt
     1141 gaaatgacca aatggaagat gagttggatc ctgtgttgtg tcaatagcct cggctacgaa
     1201 aaatctgtca ccccatgggg tgctgataag aatgcttatg taatgtcaac ttttcgcgcc
     1261 ggggcgtcca gacctgaacg actagaaagg tatgaacacg tcctctcccc ttgcacggct
     1321 gccgcacaac cctgtctggc cccttccggt aaccggttcg gtcgctccat tgcagtccgg
     1381 gtctgagtag atcgcgaacc attcatgcaa tcgttcccgc atctctctca acacgggacg
     1441 cgccgccggg tcactgatcc gattctcggt ttcggcaggg tcattgatga ggtgatataa
     1501 ctcgttcggg ccatacgggt aacggtggac gtatttccac tcacgcgttc tgaccattcg
     1561 tacagggccg tactcatcga aaacaacaag atgctcatgc gggtccgtgc gtccgccttt
     1621 gagcagttca cagaaactcc tgccgggaga tatggggtca gacggggtgg agattcccac
     1681 gtattcgagg agagtggacg aaaagtcgta ctggctgagc aaatcgctgt tcagagcgcc
     1741 tccctcaatc actcctgggt gcgatgccaa gagcggtact ctgaccgact cctcgaacat
     1801 gttgagtgga aaacttgcgt ttccctttcc ccataggccg tgatggccca tgcacattcc
     1861 attatcggaa gtaaacacga cgagagtgtt ttctcgcttg ccattctctt ccagccaatc
     1921 gatgatcttg ccgatgttgg tgtccatcgc agttattgcg gcgtagtagc cggaaagctt
     1981 ctggcgcctc gagccggcgt cgtccacagg gattgaacgg tgtttcaccc agtccggagc
     2041 gggcatcaaa tccggcgtcg acgtgaaggg acagccgtca tggtattgat cgaaaagctc
     2101 tttcggatga ttctcgcggt cccaagggct gtgtggtgag gtgtaatgca cactgaggta
     2161 aaacggttgg ctgtgcgatt ttcgctgctc gagaaaccgc aaagcgttca ttgttatcgc
     2221 gtcggtaacg tagttcggtt cttcgtagac ctccccgtgt cgcaccatgg gggcgcgata
     2281 gtacgggcct ccacctttgg cgtgggcatc ccagaacacg aacccgtccc ttgggcggtg
     2341 gcagtcgccc agatgccatt tcccgcttaa cccgcacgcg taccctgctt ttgccagttc
     2401 gtccgtgtac gctggttgtc cgacaaggaa gtcgatgaac tccccctttc tcgcgggttc
     2461 atacttcgcg atggtgttgc cggcggcaag ccagtcgtga acaccgtggc aggacggaat
     2521 ccgaccggta agcagagaag cacgcgccgg agagcacacg ggcgaggtgc agaagaaacg
     2581 gtcgaaccgc atcccggtgg ccgcgagcct gtcaaggttg ggggtttcca gctcgctgtt
     2641 tcccgtacac cgcatcgccc acgggccctg gtcgtccgag agtatcacga cgacattcgg
     2701 acgctcttca tgcccgcgca acgtcatcct tcccgccttt cattccggtc gggacacctg
     2761 ccagtttgtt ctaagagcga aagtcgcgac atcacccgcg gattcgattg acctccgcct
     2821 cgaattgtgc gatttcctcc tggttcgtca gtacgccgag ttcgagctcg aaaatccgtt
     2881 cttcctgcgg ctcgatgaac tggagtcttc ccgttgcccg gtccttatcc cgtccctcaa
     2941 caagacagtt ggcgggctca agtccgcacg tgtaccaacc ctgccccatc tgtttccatt
     3001 gaatcagttt gggaagttgt tccggcctgt agcgtatgta cccgccgatc cccatttccc
     3061 ggttcacgat tccgatagcg gtacgtccgt ccggtccggc gttcacgtca tggtagtagc
     3121 atttctcgcg gtaatccttc accggttcat gcaaacggaa atacgtttcg gcgccctccc
     3181 gcgcgtcagc atcccgcgga gttactttga ccgcaggggt cagtacttcc gagccattcg
     3241 caaccaccgg gaacccgtag ttgcaatggt agagaatcat cagaggtgtg gtatgccacc
     3301 cgatattcac gactttgtcg cgtatggtga acgaattcgc ccccagtttc gtcgatatga
     3361 cccgttgcag ttcgagctcg tcctggaaaa ggccgctttc gcgcatcacg cctgaagccc
     3421 agagtatgta ctcatcgcct tcccagtacc cgccgaaatt cgctcgtttg gccggaacgt
     3481 tgccaatacg tccatgtatc ccgagttttt ttccttcatc ggtgcatgcc gcccctgcat
     3541 agctcagacc gcacgtcgcg acgagaccac cgaaaaactg gcgcaaccat tccaggcctt
     3601 ccgggtcgta gaaggatccg tgggggtctc cgacggagga acgccatgcc agagcctttc
     3661 cctggtaact tgccgcgacc acatccatcg cgcgtcccgc tgcaaccgtg aacgcgaaac
     3721 ccgaacctgt agcgaaatcg aaaacctcca cgccggtgtt gtttccatct gccagcacgc
     3781 tcttgcgcac atctgctatc tgggatatat caccgacacg ttccaaaagc tccctcttcg
     3841 tccagtgttt tccccaaaga ttcgccattt ctctgctgtc ctccaaaatg gtagtggtgt
     3901 tttcagttgc tgtccatgta ctcgaattct acccgatgat ctgggaaagg gcacacaact
     3961 gtctgcctct ggttgcgatc ctcaattggc tgaggtggaa aagcgcgttg ccccgtcgag
     4021 cgctgacttc ttcagaacaa gcccgtgtcc agggcggaga gggacagtaa tagccccgtc
     4081 gtaaaaaacg agcggttcct cgaaaagttc gtccaattcg ggtgattgac cggtgtactc
     4141 ctgtggcaaa tcgcaccagt ggagcgcggc acatatgtgc acgttgacgg cggtcccaat
     4201 cgacggttga gtctggtgcg ggaggaatag cctgttgaag acctccgcca aggtcgcaat
     4261 cttcatcgtc tccgtgatgc cggcacactt caccacgtcg ggttgaacga tgtccacccg
     4321 cgcgttcaaa atcaaatcac gaaactgcca ccgggtgtac tcgtgctcgc ccgcagaaac
     4381 aggcaagtcg agtgcatcgg ctaccttccc gagcccttca tagtcaagct gagcgaccgg
     4441 ctcttcgaaa tgccgcaacc ccaacgcttc aaacttgcgc ccctgtcgga tggcagtcgg
     4501 caccgagtac ccgttgttag catcgaagct cagaggaacc gctgtgccac atacctgctt
     4561 gcattcctcc accatgcgaa ggtctttttc gggccctctg tcctgtcggg caacgcccca
     4621 atccatgcgg attttcacac ctttgtatcc aagatgtacc gccttttcaa ccctcgttgc
     4681 catttcgcgc ggcgtcattg ttgccccgcc cccgattgac gcgtacatgg ggatgtgctc
     4741 tcgatagcac ccaccgagca tcatgcacaa gggctgcccg gtagccttgc cgcgtatgtc
     4801 ccacagggcg atgtccacac ccgcgaccgc ttcgggttgg attccctgaa cgccgagttt
     4861 gtacgtgtcc agcatcatca catgccagag cttgtccgtt tccagcgcgt ttttcccgat
     4921 tattcgggga ccgagaactt ccgccacgaa gcgcgccgtt gtttcgctgt gcatggggct
     4981 gcactcgccg acaccttcta ctccctcttc ggtctgcacg acgacgaaac acttaccgtg
     5041 gacgacgtat gaagaaacac ttctgatgtt catggttccc ttcacggtca ggtgctgatc
     5101 agtcaagaga agtcacccgg atgttgctga cgtccaccac gatctgagag ccaatagcac
     5161 gaaacccgat cttgccgctg tcgggaagag gaattggcaa cgtctgcggg tctacgaact
     5221 cgtgggcgag tactccgttc acgccgagct gaagatgtgg tcccgctttg acgaccgtaa
     5281 gatggtagaa cgtcttgatc tgccggcagg ggtccggacc ctgtgccatc agatgcaacc
     5341 ccggattcct cctccagttg gagactccgg tatgttcgcc tttatcgttg taccggctga
     5401 cagacacgtg gtaagaacgg agaagagcgt ctttggccac atagtctccg aacactcctg
     5461 aacgcggtgg cagcgaaaac atgtcctctc cattcaggcc gcgcaccgcg acaaaggtga
     5521 tgaagagccc gtcgctgaaa tgaacgtaga ggtcaaagtc cagcacgata ttgtccggga
     5581 agtccttgac atggaagaaa ttgcatccct ctgcaccctg aatgcttccc accgagtcaa
     5641 ggcgcaatgc gcgttctccc cgcgcattct ctaccacgcg aggactccca attccttcga
     5701 gccgccattg cgtgagccct tcatcgaagg tctcgtgaaa aagagtctgt ccgcgaggtg
     5761 tgaggagacc catagccgcg ctcccacggt tttttggaaa gggggaacat tggttctgcc
     5821 cgagattatc gaaatataga aaacgtgttc cgcacgcaga acccgtggga cagaaatcac
     5881 ccttcttgac gcttatcttg ttctcggcct atattcccaa ttcagcgccg gacaggggtt
     5941 cttgcgccgc atacggcggc gctgtgtgcc acactccggg ctgcggccgg ttgttccctc
     6001 agattcttgc aacgatctcg ttggtgtgtg gtgctttttt cctgcggagg atgggcacat
     6061 ggaaaagacc attggtgtcg gaatgattgg ttacgcgttc atggggcggg cccactcgaa
     6121 cggatggcgt caggtcgggc actttttcga tttgccgatg aagcctgtga tgaagacgat
     6181 ttgcgggcgg aacaaagcgg ccgtgacggc tgcggcaaaa acgatggggt gggaaaaagc
     6241 agagaccgac tggatgaaga tgatcaagga tccgcagatt cagatcgttg acatctgcac
     6301 tcccggaaat gaacacaaac cgatggccct cgccgcgttg aaagccggga agcatgtggt
     6361 gtgcgagaaa cctctcgcga acaccctggc cgaggcgaag gaaatggcgc acgcggcgaa
     6421 agaggccggt gtgtgcacgc tcaccgcgtt caattaccgt cgggttcccg caatcgcgct
     6481 cgccaaacag atgatcgacc agggcctgct cggaacgatc taccactggc gtgcggtgta
     6541 tctgcaggat tggatcattg acccgaagtt tcccctcgtg tggcgattga ggaaggaagt
     6601 tgccggttct gggccccacg gcgatctgaa cgcgcacatc accgatctcg cccggtacct
     6661 ggtgggcgaa atcacggaag tggtgggtat gggcaaaaca ttcatcaaaa cccgtcccat
     6721 tcaggaggcg ttggcggacg gcgggctcgg tgcaaaggcg gggacgaaga aggggaaagt
     6781 taccgtcgag gacgcgatgt tgttcctggc gaagttcgag aacggggcgt tgggatcgtt
     6841 tgaagccacc cgcttcgcgg cgggaaggaa aaacttcaat tccttcgaaa tcaacggctc
     6901 gaaaggttcc atcgcgttca atctggaacg cctgaacgag cttcagtact tcaatcgcaa
     6961 agacgatgcg aatctccagg ggttccgcac cattctctgc aacgaaggcg ggcagcctta
     7021 caatgcagcg tggtggcctt ccggtcacat catcgggtgg gaacacacgt tcacccatga
     7081 gttcaaggat ttcatcgagg ccattgcagc gaaaaaggac acaatgccga atttcgaaga
     7141 cggaatgaaa acgcaggcgg ttcttgaagc agtcatggct tccgcagaca agaaaaaatg
     7201 ggtaaaggta ccactctgaa cgggcgcttg aataacgccc ggaatgtccg gaacgcaggg
     7261 atgccgtccg ccggcatccc tgtttgcatc tcatacagaa agggcaccgg cagtggcaaa
     7321 cgaagccaaa cgtatgatac ttgaggtgaa acaccgaccg ggtaagcccc ctctggaatg
     7381 ggcgtgtgag tgggtggatg gtgaggttgg caaatgggcg cgcgtccggt acgtgagcga
     7441 caccgaacag aaagtgcagg ataccgtgct tccggtcggc accgttaccg acggggtata
     7501 ctgggcggac cgtccttttc acgtatggaa gttctcaacc ggtgcaggtg ggcacatcgg
     7561 gtaccgtttc gatgtttgta cgaacacgtt catctggaag gaaaaactga tttggtacga
     7621 ccttgaactc gatctctgga ttccttgggg cggatcgccg cagtggcagg acgaggatga
     7681 catgagacga cttgtcagga tggagcatct cagtcaggag gaactggagg ttgccgaaca
     7741 ggcgaagaag tatcttgaca accactggag aaccgtgatt gagtgtgcgt tcggggaggt
     7801 gcactcttag aaaacagggc aacaagtttg cgccggggag gcggtttctt ggcacgaagc
     7861 tcgaacgatc gcattctcat ccttcagaca tcaagagccc cggtggcact ctcggatgaa
     7921 cgcttcctcc gttcacggtt cgatgtacga gcttttcgta tcattcttgc gccgcccgtt
     7981 cgtgtgctga ccaacacggc aaccctagct gtatggttgt gctggaacct tccgcgagca
     8041 cgtggggttt tcttccgctt cgcggattac tacgcgttcc tgcccgtgtt tctgtcgcgg
     8101 ctgttcagac gcaagtcatg gatcgtcctc ggcggatacg acgcccacca tttcccggaa
     8161 tatggctacg gcgtctacga gcaaccactg cgagcatcaa ttgttcggta cgccgttcgg
     8221 aatgcaaccg cagtgttgcc cgtggaccag tcgttgtacg atggcgtgaa cgcgtacgcg
     8281 ttcgatccac cccgccccac ggggatcaaa tcgctggttc ccaatttcag gagcgaagtc
     8341 cgggttgttg cggacgggtt tgatcacgaa ttctggacgc cctctcccgg atgtcggaaa
     8401 gaacggctgg tggtggcgac cgcgagtgtt ccgcagagta cctcgcggga gagccgcatg
     8461 cggatggtgg tgctcaaagg tgttccgctt ttgctggagg tcgcccggcg gatgccggac
     8521 gtcaccttcg cgattgttgg tcccgaaggg ggagttcttc ctcagggggg ccaggccgtt
     8581 ccggcgaatg tccagttgcc ggggtatctt tcgccggagg aactccgcga gtattaccgg
     8641 agagcggccg tatatgcaca tccgtctctt accgaggggt tgcctgctgc cgttgcggag
     8701 gcgatgcttt gttgttgtgt accggtcggt tcctcggtga acggtattcc ggaactgatc
     8761 ggggatacgg ggttcgtact gacgcggccg gcggttgacg aatgggtcgc cgcggtgact
     8821 cgtgcattgg aactcggtgc gtcttcacgg gcgcgagatc gaatcgttgc tcttttcagc
     8881 ctggcgaagc ggcatcgcat gctgctcgat gtgattgagg gatgagtctg gtggctatcc
     8941 gtcggggaag aagggtcgaa tcagaaaacc gttggaaggt gtcataggag aaggcagatg
     9001 ccatcaatcg cgattatagg cgcatcgaaa gaccgttcga agttcggtaa caaagccgtt
     9061 cgggcttacg ttcgtcaagg ttacaccgtg tacccggtca atcctaagga acgcgaaata
     9121 gaagggatcc tggcgtatcc ttccgtgaag tcgattcctt ttccggtgga cagggttgcc
     9181 ctttacgtgc ctccccagat cggcatcgca ctgctacccg agatagccgg aaagcatccc
     9241 gcggagttgt tcgtcaatcc gggtgcggaa agcgaggaac tgctggacga agccagaaga
     9301 ctgggtctgg aaccgatcgt tgcctgtgcg atagtgtcag tcggggaaat gccggattag
     9361 gatggacgga cagcgatgag aaaggtcgtg aaggcacggc ctcggcttga gaactcgcca
     9421 tcggacggag cgaaagcgct ctttctggag gcggtgcgcg cgctccggcc ggaggacatc
     9481 ccggtcaaac cggttgatcg tggggaaggc gacgcctctg aaccagttgc cagacgtcgt
     9541 actgagaggg ttgtggagtc tatcgatctt cacggcatga ctgcagaaca ggcgatcagg
     9601 tatctcacgg aggtgctccc agattaccga cgaacgggtg gaacggtcca gatcgttgtc
     9661 ggcaaagggc tgcacagccc cgacgggcag ggagttctgc gcgaggtcgt ctctggctgg
     9721 ctcgaccatg caggaaaacg tttcgtctcg cgatggcggt ggggtgggcg gagacaaggg
     9781 ggaaatggtg tcatcgtcgc ggcaatccgc gttcctggaa aaccagactg aaagcagggt
     9841 ggtgtcttga accggaatct tcttcgggaa ctcatcgagc cgatcctcaa cactcctacg
     9901 gggccattct acgaggatca cgtggcgtcc ttcgtcgcgg aaacagtacg cggcacagga
     9961 ctcacgatga gcactgacac gttcgggaac atccttgtcc gccgcccctc agtcggcagg
    10021 aacaacctcc gtatcgggtt tatggcgcac atggatcacc cggcctttga agtgatagag
    10081 cagctcgagg gagcagttcg tgttgcgtgg cacggaggtg tggatgcgga gtacttcccg
    10141 caggcgcgcg tcagatttca cacccccggc aatccgtgca cgggtgagat tcgagaggtg
    10201 gagcttgaag aagctcgtaa cagggtgaaa accgctgttg tggcgtgcga ctcacctcca
    10261 tccgttggga ctctcgcaac gtgggaactg ccaccggtgg aggtaacgaa cggggttctg
    10321 cgtgcgccgg cggtggatga cgtcgcggga tgtggccttc tgttggcgct actcctcgaa
    10381 ctgggcgacc gaccgctgga aacggaggtt tggacccttt tcacccgagc agaagaagtc
    10441 gggtttgtgg gtgcgatcgg actggcgcga agcgggcgat taccagtttc gacgcccgtt
    10501 gtttctgtgg agatgagcaa agcgacggcg ggcgtgacgc aggggatggg tccggtaatc
    10561 cgtctcggag atcgatcaag catttttgac gcaatgttcg tgctcttcat gaaagacgtg
    10621 gccagcgacc tcaaagggcg tatcgagggc ttcaaatacc agcaagcgat gatggaaggg
    10681 ggcagttgcg aggcaactgc gctcaatctt tttgggtacc gaaccgccgg tctggcagtt
    10741 ccactgacaa actaccacaa ccagcgccct tccggcccga gtggcccctt cgtcatagat
    10801 tcagaggaaa ttgccatcaa tgactttgca aatgccgtgg atctatgcgt tggaatgtgt
    10861 gaacgatttc gcggccttga cgcgatcagg agcgggcaca gaagcaaagc cgtggaacgc
    10921 gcagcggagg gtttgcagcg tctggaacga atggagcgcg caagaacacg cagatgagtg
    10981 ctcagttgct cgatgaacgg ttcgagcgtt gcgttaccga cttcaggtca cgtcctcccg
    11041 tccttctctc tccagcgctt ctaccaggtt cttcacctcc tgtacccgcg acttcgggca
    11101 caccagcacc gcagcgtcgg tttcaatgat cacaagatcg tgcactccca tcgtcgcaac
    11161 gacggagttg ctggttcgga taatacagcg ttcggtatcg agacccacgt gttggcctcg
    11221 taccacattg ccccgggcgt cgtgaaggct tatctgatcg agagcagccc acgttccaat
    11281 atccagccat ccgaaatcag cccggatcac ctcgacgtta tctgcctttt ccatgatgcc
    11341 cacgtccagc gagacccggt cgaaacgttg gtacgcgttc tcagttacgg caggttcgtc
    11401 gggcgttcca agagacgatt tgatctcttc gagcagggcg aagtggcggg ggaggaagcg
    11461 cgagaaaagg ttcagtgcat gccgtgcctg catcacgaac atcccggagt tccacagata
    11521 tcgcccgtct tcgacgtact gaatcgcttt ggccttgttc ggtttttcca cgaactcgtc
    11581 gaccctgtag aactggattc cctcgatttc acgtgtttcg tggccaaaac gaatgtaccc
    11641 gagaccggta tccggtctga gtggtacgaa ccccactacc acgacacaag gagcgttttc
    11701 cgccacggta accgcggcgt gcaggacctc gcggaacccg gtttcgtcgg cgatgaaatg
    11761 atccgcggtg actactgcca tcgtcgcggc cgggtcgcgc tcggttacct tcagagccgc
    11821 ccatcctatg cacggggcgg tatctttgcc caagggttct accagaatgt tttccgcggg
    11881 aaacgccggc agagcacgag ctatttccga tgcatactcg cgcgtcgtta cgatgatctg
    11941 ccgctcaact ggtatgagcg gggcaagccg atcgacggcc acgcgaatca tcggccggtg
    12001 gtctataagg tcaagcaatt gcttgggtcg ggttttcctt ccttcaggcc aaagccgcgt
    12061 accttttcca cccgccatga ttacgccgta cgtcatgcgt cccccggggt atttaacgtg
    12121 gcgcttggtc gccggaagga tcatctagcg gcagagatgt caaaggaccg agggccccga
    12181 ggtccccatc gaaccgggta gagtcgccga caacgagaac gcgcagttgg tcgcttctca
    12241 ggtatttccg cgccactcgt accacgtcat cctttgttac ggaacggatc cgagggatgt
    12301 aagacgtgag ataatcctcg gggtacccgt cgaacacaag gcgcatctcc tgtccggcga
    12361 ttcgttcggg cgaggcataa gagaacacaa acgagttgat gatagtctct ttcgcggcgc
    12421 tgatttcgtc gtcggtaaca ggtttgatac tcatgctgtc aatcacggac cgcattacgg
    12481 agatcgtttc tgatgtcttg tcagcacggg tgccgaccgc tgcaagaaac atcccctgat
    12541 cgggatcagg gctatccagc acgattccga atacgtagta cgccaggccg cggttcgaac
    12601 gaacctcctg gccgaggcgg gacgtgaagg ttcccgctcc gaatatctgg ttcatcacgg
    12661 cgacggcgta ctggtctgga tcgtgtcttc gcaatcctgt gcttcccatc cggattgcgg
    12721 actgattcac tgctttctgg atgtgataga cccccggagg cattccgtgg ttctgctcag
    12781 gtgatggcag cggcggaacc tccgcaacag cccacccggc gaaagcccga ctcactttgg
    12841 cgacggcctc ttctggtgta atgtctccag aaatcgccat gtaggtcacg ttcggtctga
    12901 accacgtccg gtggaacgcg acgagatctt ctctggtgat cctgtcgaca tcctccaatt
    12961 cgagctgtcg tgcccaaggg ttgccttgtc catacacgat ctggcggaaa cggcgccttc
    13021 cgatctcgta ggggtcatca ttccgccgac gaatgctttc cttcgtcaat cgttggagtt
    13081 caacgatctt gttctcaggg aatgccggcg aaatcagcac atcggcaagc atggcgaggc
    13141 cggagtcaaa atccttgcgc agcacattca gattggcact ggcgcagtcc acgtccattc
    13201 ccacattcag gtgtgctgcg aggtaatcca gcgtgtcgtc cagcgcgtcg gcgctcaaat
    13261 gcagcgttcc accgcgtctc atcacgcctg cggtgaacga ggcgaccccc ggcttccctg
    13321 cggggtcaca ctgggatccg cctctgatca ggatgtcgac attcacaatt ggctgctcgt
    13381 tgtcggtgag aacgaacagc gtaatcccgt tcggcagcgt ggttcggaaa accggcggcg
    13441 gcgtgaactt cagcgagtcg aacggaggga ggtcgtcggg gtgtaacgca acgggacgag
    13501 gggtcggctc ggtttggcgg accggcaggc acgaactcat gaaaacggtg aaggccagtg
    13561 ccgcgaccag gtgatggatt cgcaccatgg ttactggtcc tcccttccgc gtccattgtt
    13621 ttgctcattc tcgcgaacga tggctgctac cgtccgattc tggggtttga aatacaacct
    13681 cgccacgcgc atcacgtcat ccgccgttac cttggagaca ttcgctcgat gctcagtaac
    13741 cacccgccaa tctccgtgga ttgcctgcgc acgcgcaagc gcctgagcca gtgaattgtt
    13801 gtccgagatt tcgcggatgt acgacgcctc aagctggttt ttgcattttg tgagctctgc
    13861 ctctgagaca ggcaccctcg ccaggctgtc gagttccgag taaagaatct cttccacttc
    13921 ccccgtcgta tggggcgctg ccggcgaggc gtgcagtaca aacagatttg ggtacctctg
    13981 gccgggcagt tcttcgtaca cggccacatc gctggcgata ccggtctgga cgagtctgcg
    14041 gtacagcctg gaggagttcc cgctatcgcg gaggatgttg gcgattacgg aaaaaacata
    14101 tgcatccggg tccgggtaat ttggtttgtg gtatcccacc atcaactgcg gttccgcatc
    14161 gaagtctacc g
//
LOCUS       DAOTNG010000037        14163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4207_length_14163_cov_5.633258, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000037 DAOTNG010000000
VERSION     DAOTNG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_14163_cov_5.633258"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="P5179_03015"
     CDS             complement(<1..1495)
                     /locus_tag="P5179_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRS94223.1"
                     /translation="MNSIAHGLFRRVAEKWVAFSLRHLWLTAAVSLAFTGLALWAASF
                     LSLKTDLATLLPRDYPSVKELDRVQQKVGGLEKVIFVIESPDSAANHRFALALGQKLL
                     ADSAYSYVEWSRDLSFFDDHKLLYMDLEDLGEVYRRVSSKVQMDEFGEPLDFSDIEEK
                     YKGTNGSGNNSNGSDGWATADGTTRILIAYPAGQSGDVGFSRKVIASARKQVAALNPA
                     SFHPQLEVYYGGEFKNRVDEYESLVSDIKSTAIISILGIIITISLYFRQYFAWLYIGY
                     PLIAGLGWTFGITALTVGHLNLITAFLIAALTGLGIDFGIHVFQRYTEERGRGQDLET
                     ATTRAIVNTGSALLTSATTTVVAFYALTITDFKGFSEYGFIAGTGVLMTLTAVLFSFP
                     AFIAIGERLNLVRHRHAAKKWAESTATFPFAKAVVYGTVFLLAIAVFAAAFYPRVRFD
                     YDFSNLRSNIPASNLVKSKISKIQPMESAPAAIVVEDSAARREVADAL"
     gene            complement(1746..2099)
                     /gene="erpA"
                     /locus_tag="P5179_03020"
     CDS             complement(1746..2099)
                     /gene="erpA"
                     /locus_tag="P5179_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRS94224.1"
                     /translation="MADAPVAVDSLVTISESAAKKIRELLQEEGNPNYALRLQVVGGG
                     CAGFQYKLAFDDEFAEDDELLDYEGFRVVVDPMSARYVEGATIDYVESEMASGFKIEN
                     PNVVTTCGCGHSHSF"
     gene            2409..4688
                     /locus_tag="P5179_03025"
     CDS             2409..4688
                     /locus_tag="P5179_03025"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRS94225.1"
                     /translation="MNVPEETEKARAETAQCDRLFAEIMQLASRFDKNIDLPTLRRAY
                     RFASERHKGHFRSSGEPYLVHPVHVARILVDLHLDTQTLAAGLLHDVVEDDLKTPIEE
                     IEVNFGNDVATIVDGVTKISRMQLVSDKENQIDNFRKMLLAVAKDVRVILVKFADRLH
                     NLRTLDYLPPEKRLRIARETLDVYAPLAHRFGMARVRRELEELAFRYADPEACARLEA
                     QVAQRKDEYWNAIEQFCEEIREGLSKRGMPAEVSGRVKHLYSIHNKMVSEGKDLSEIY
                     DLLGVRIIVETADQCYDALAYLHQHYRPLEGRFKDYIGNPKSNGYQSLHTTVTNNQRT
                     VVEVQIRTREMHEVAEEGIARHWLYKEGRATGEIDAQMRWVRQFLEWSKDIRDPNEFY
                     EQLKIDLFPDEIFALTPKGDVIPLPKGATALDFAFAVHTEVGLHASGAIVGGRIVPLG
                     YELQTSDSVRIITSANQKPSRDWLNLVKTAKARTRVKQWLRQAEFALNVEIGRDLVEK
                     RLKKAGLVLAEEEWGAVASAFNTKDREHLFAAVGAGDLAPERVFDHIRSMIAPAPTKE
                     EAPPKELPATDSEAGGIVLSGMDNPLVRIARCCNPVPGDPVLGFITRGRGLTVHHREC
                     PNIRQLKEDAGRLIPVRWEAKDVIGGAGPFKTAIIVKGSDRVGLLSNVTSTIASTGAN
                     IRHAEIRTLPDGAHSAFLLEVKNLKQLKRVISKLRKVPNVVEVHRLATEGDDEDELRA
                     MIAGSPLPFVEESPDDEDE"
     gene            4690..5469
                     /gene="pgeF"
                     /locus_tag="P5179_03030"
     CDS             4690..5469
                     /gene="pgeF"
                     /locus_tag="P5179_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRS94226.1"
                     /translation="MFRLTSETGLPMLRSSLLDELPGVWHGFTTRIGGVSAGSFAFLN
                     LSLNVGDSPDAVLQNRSLLARSAGISPEMVTLQAQQHGSDVVLCDSASPEAAHRADAF
                     IAAREAIPVLVGTADCLPVLLASEDGRIVASIHAGWKGVLAEVIPRTVTAMQTVSGAR
                     PESMRAAIGPHIRACCFEVGDDVAHQFRKEHVVAPHARGGKSYVDLSAVAVCQLADSG
                     IAAKNIDISSTCTVCRHDLFFSHRANGGTTGRTVAMIMRRR"
     gene            5514..6326
                     /locus_tag="P5179_03035"
     CDS             5514..6326
                     /locus_tag="P5179_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRS94227.1"
                     /translation="MSVSEAAIVGVIQGMTEFLPVSSSAHIVYAEHFLGIKEGNVDLA
                     IVVHLGTLVAVFAAMWYRIAPIIRGTIAGVADLAVRRSPWAREEFRRATYIIAGTVPA
                     ALLGVLFEDSLKETFGNPFQTSVLLIGTGIILFATRFVRRNDRDWGLSPALAVGFAQA
                     LAILPGISRSGSTISMGLFLRIEREKAAEFSFLLSIPVILGGAIIHVAGMVGQPGGLA
                     LLSGPLIAGFCTSAISGYAAVRVLVSFVKKGALHWFAVYCWIVGAAGMWYFA"
     gene            6337..7164
                     /locus_tag="P5179_03040"
     CDS             6337..7164
                     /locus_tag="P5179_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRS94228.1"
                     /translation="MHNQFADIRFMSFNLRFPCESDTGNLWDERRARVFEMIQLYDPD
                     VIGMQEVYRRQLNDLLSALPGYAAVGKERYGGTDEEHNPVFFRRERFLAEGSTTKWFS
                     DTPDVPGTRTFCPSDHPRIVTSATLMDIADGFRFSVLNTHFPLTRDEDIQLACARVVE
                     SQLALVPKSAPVVLMGDFNAPPDGGLHGFFTRSGGGIGEGFRDTRVLAQEVLGPAGTV
                     HGFRGGMLPESRRIDWVLLAGEGQVTRYETVGFERDGKYPSDHFPVCVDIRFAQPEN"
     gene            7189..8163
                     /locus_tag="P5179_03045"
     CDS             7189..8163
                     /locus_tag="P5179_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS94229.1"
                     /translation="MERPRVYVHRVGSWAPRYLDDQNRSRLADFAEIADDASLDAPPP
                     DIAHRLEGVQAILSLNGAGAEDITEDALRAVGTVRIAVISHWFHGSHDRATAAWRAAG
                     VRVTDASWGNNFAVAQWTLGAMINGVFRVAELDRAMRAGEVWPDHHFTSSMLDGQRIG
                     LVGLGRIGRLVAGFLQPFHVEIVGYDKYVTAEEASKFNVRWCPLEQVMATSDIISLHL
                     PVTPETTRLITRAHIESIRKGALLVNSARTAILDYEAFRECLQKGLFRAIVDVFEPEP
                     PPVDDPLRTLPNVVMTPHIAGSTARMCHVCGRTAIEELRKQLVGQSSG"
     gene            8238..9209
                     /locus_tag="P5179_03050"
     CDS             8238..9209
                     /locus_tag="P5179_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRS94230.1"
                     /translation="MSHRIPVFKKAPRATIPWEHDPTTGRTPQARSKSVLTGIGIGLS
                     VLLVCLKGFAGWRSGSTAVISDAFNSVLDIISYSALYLSVRLQAKPADLTHHYGHHRA
                     EPLAGFLIAVFAAILGGMVLRDSVLRLLRPDPVRMSALSVIIVLFAIVSKVIIAIAYR
                     RGAKGGSPAMDAAAIDSRNDAMASSVALIGLIGGAVWPYLDGAAGLAIGAWILGSGVR
                     VGLENIGFLLGKAPSKAVLEELRAVAETVPGLIGTNNIRAHYVGNSMHVEVHIEVDAN
                     LSIREAHDLAKTVGQRLERLDDVYAAFVHTDPVDRGLKNPQQSEQAS"
     gene            complement(9241..10326)
                     /locus_tag="P5179_03055"
     CDS             complement(9241..10326)
                     /locus_tag="P5179_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS94231.1"
                     /translation="MNVLLPENAYSVILAGGGGTRFWPQSRSQKPKQFLPFFGPRTLL
                     QHSFDLITPLTPPDRILVVTNSAQAGLVREQLPDVPEANVLSEPCARGTAAAVAWAAA
                     AIAARNAKGTMTVVGADHWVRNPEALHAAVVRGHEVAERGHALVMFGLTPGGPNPHYG
                     YIIGAPEPEESELAATVRVLRFHEKPPAETARRYLSQKHCYWNSGMFSCSVETMHAEM
                     DLYAPNILRAVRAAIRSLPDHDAFREAYSGIPANSIDRAVLEHSANCYVVPTAIERVE
                     LRTWASLFSLLPRDAGGNVSIGDVRMLDCANTVQFGSGKLVAAIGMENCAIVANADAV
                     LVCALDRADDIGSLIYLAVEKGYEELR"
     gene            complement(10396..11640)
                     /locus_tag="P5179_03060"
     CDS             complement(10396..11640)
                     /locus_tag="P5179_03060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS94232.1"
                     /translation="MSEKLAIHGGTPVRTKPMPPANPGECIIGEEEKEQVLEVLDAHS
                     PFRFYGPDLRKKTEQFEAEFEEFMGVKHALGVTSGTAALKVALAAAGVGPGDEVIVPA
                     VTFIACAGAVVMSRARPVFANVGTDIGLDPADVERKITKRTAAIMPVHILGGAADMDP
                     ILEIGKKHGVKIIEDSAQSAGATYKGCRVGSMGDVNAYSLQFQKVITAGEGGVVTTND
                     EELFDRAVRAHDHGINRWNNVAEKNFCAEVYRMSEMHGAFALAQLRKLESNLAAMRRH
                     NRRIRKAVEELPGLTLRTLADVEGATGQSITFFLETADLAAKWREALEKEGIPNFVLY
                     GGTMVFENPQVLHQRMPTKHACPFDCPLYEGPITYAKEDYLQSREILDRSVWLYVSPL
                     LTDDDCDDIVKAIRKVHTGLSA"
     gene            complement(11712..13010)
                     /locus_tag="P5179_03065"
     CDS             complement(11712..13010)
                     /locus_tag="P5179_03065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRS94233.1"
                     /translation="MTFSEATRYLNALIDYEKSPDFSYATAFKLDRVQSLMATMREPF
                     RSFDSVHIAGTKGKGSVAIYCEALLRASGIRTGLFTSPHLVDFRERIRVNGARISESL
                     FADITTEIQPHVARWQADHPDDRLSFFDLLTAIGFEAFRRSEVEIAVIEVGMGGRLDA
                     TNVLLPKVSVITPIALEHTRFLGPTLEAIAAEKAGIIKERVPVVVAEQKPEAAAVIAR
                     VAAGKHAPSTWVGRDITFDETGEPWAVRLKARSVDGVALKMQGVHQRHNFATALAALD
                     EIGCVPAEAVVRKVASETIIPGRMQVLPTRPPCMLDVAHTPESAACLAEGIRQTFAGK
                     QVTLVFSCASDKQVEGIAQVLAPVAGNVVIVPMSGPRAMSMERMYDAWSRAHTRVERA
                     ETISAAVDRLKDAGSDSAWVVCGSFVLVGAAMQLLGYSPP"
     gene            complement(13258..>14163)
                     /locus_tag="P5179_03070"
     CDS             complement(13258..>14163)
                     /locus_tag="P5179_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRS94234.1"
                     /translation="VVFFAISKKVLGSSNYQMMARGHVQGSSSPLPRRWVPVVWVSIA
                     AVIGCALVPHASVVLSAIADTWFMTILPSSYTTVYIGRVFTHPLTLSSIRNSLFLSSL
                     STLVDVALGVTIAYFLARKRFIGRDVLDGLAMLPLALPGLVLAFGYVAAFSGSFLDIK
                     RNPMPLLIIAYAIRRLPYMVRAAYAGLQQTSETLEEASLNLGAGPLRTLRRITLPLVF
                     ANIVAGSVLAFSFAMLEVSDSLILAAKEEFYPITKAIYAMSLRVADGPYVASAMGLLG
                     MVLLATSLIVAGRFLGKRLGELFRA"
BASE COUNT         3067 a         4041 c         4179 g         2876 t
ORIGIN      
        1 gcagagcgtc tgccacttcc ctccgcgcgg ccgagtcttc gacaacaatc gctgcaggag
       61 cagactccat cggttgtatc ttcgaaattt tcgactttac gagattcgac gcagggatgt
      121 tcgaacgcag gttggagaaa tcgtagtcga atcgcactcg cgggtagaac gccgcggcaa
      181 acactgcaat cgccagaagg aacaccgtgc catacacgac cgctttcgca aacgggaagg
      241 tcgccgtaga ttccgcccat tttttcgcag cgtgccggtg ccgcacgagg ttcaggcgct
      301 cgccgatcgc aatgaacgcg ggaaaactga aaagcacggc agtgagcgtc atcagaactc
      361 cggttccggc tatgaagccg tactcggaga accctttgaa atcggttatt gtgagcgcgt
      421 aaaaggcaac tacggtagtc gtcgcactgg tcagaagagc ggaacctgtg ttgacgatcg
      481 cacgcgtcgt cgccgtttca aggtcctgcc cgcgccccct ttcctcggtg taccgttgga
      541 acacgtggat gccgaaatcg ataccgagac ccgtcagcgc ggctatcagg aaagccgtga
      601 tcaggttcag gtgcccgacg gtgagggcgg taatcccgaa cgtccacccg aggcccgcga
      661 tcaacgggta cccgatgtag agccacgcaa aatactgccg gaagtacagc gagatcgtga
      721 tgatgatacc gagaatcgag atgatcgcgg tggatttgat atcgctcacc agagactcat
      781 attcgtccac gcgattcttg aactcgccgc cgtagtacac ttcgagctgc gggtggaacg
      841 acgccgggtt caaggctgcg acttgctttc tggcggaagc gatcaccttc cgtgaaaaac
      901 cgacgtcacc ggattgaccc gccggatatg caatcaggat ccgtgttgtg ccgtctgcgg
      961 ttgcccatcc gtcagaaccg ttcgagttgt tcccggaacc gttcgttccc ttgtacttct
     1021 cctcgatatc cgagaagtcg agtggctcgc cgaactcatc catttgaacc ttggacgaaa
     1081 cgcgacggta gacctccccc aggtcttcaa gatccatgta gaggagtttg tggtcgtcga
     1141 agaagctgag gtcgcggctc cattcgacat agctgtatgc ggagtccgcc agcagtttct
     1201 ggcccagcgc aagtgcgaac cggtgattgg cggccgaatc aggggactcg ataacgaaaa
     1261 ttaccttctc aagccccccg actttctgct gaacgcgatc cagttccttc acgctcgggt
     1321 aatcccgggg cagcagggtg gccagatctg ttttcagaga gaggaatgag gccgcccaca
     1381 acgcaagccc ggtgaatgcg agcgatacgg cggcagtcaa ccacaggtga cgaagactga
     1441 acgccaccca cttttcggcg acacgccgga agagaccatg cgcgattgaa ttcaacgtaa
     1501 attcctcgaa cttgagccgg tgactgaact gggccatgcc agccgtggtg tgtcggacac
     1561 tttgctgcgg agcattgaaa aagcaaaaac aaggccaggc gtagagcccg taccggaaaa
     1621 agccaccacg cgtcgctcag gtgcaaaaaa aagccatccg gtcatcaaac gatatcccga
     1681 atggcgttat aagcgaccct taccggcgcc gggccagaac aagaacatgc gcgcgaacaa
     1741 aatgctcaga atgagtggga atgtccgcac ccgcatgtgg tgaccacgtt aggattctca
     1801 attttgaaac cggacgccat ctcagattcg acgtaatcga tggtggcgcc ttcaacgtat
     1861 cgcgcgctca tggggtcaac gacaacccgg aatccctcat agtccaggag ctcatcatct
     1921 tctgcaaatt cgtcgtcgaa cgcgagcttg tactgaaacc ctgcgcagcc gccgcccaca
     1981 acctgaaggc gcaacgcgta gttcgggttc ccttcttcct gaagcagctc acggatcttc
     2041 ttggcggctg attcggagat agtaacgaga gaatcaacgg caactggtgc gtctgccata
     2101 acttgcctcc acaaacctgg tatcaaccgg tgcgattccg cactggttat gtcaccgaac
     2161 aaatataccg atgcgcactc tgggcgccaa acgctcgctc acacgtaacg aacaacaacc
     2221 gggcgaaacc ggcggttcct cgttgcttct gaatgggaag acaaaacgga aggcctcaga
     2281 gttccggcaa tatggttctt gcgctatctt taatgcgcgc tgtggcagcg cacaggtgca
     2341 cctgccgttc ggcaaatcaa ttgccggata aggataccag accgcagggc ggacgcattg
     2401 gatctgcgat gaacgtgccc gaagaaaccg aaaaagctcg tgcggaaact gcacagtgcg
     2461 accgcctgtt tgcggagatc atgcaactgg cttcacgctt cgacaaaaac atcgatttgc
     2521 cgaccctgag gagagcgtac cgcttcgctt ccgaacgcca caaaggacat ttccgctctt
     2581 ccggcgagcc ctatcttgtg catccggtgc acgtggctcg catccttgtc gatctccatc
     2641 tcgatacaca aactcttgca gccggtctgc tgcacgacgt cgttgaagac gatctgaaaa
     2701 ccccgataga agaaatcgag gtgaatttcg gcaatgatgt cgccacgatt gtggacgggg
     2761 tcaccaagat ctccaggatg cagctcgtca gcgacaagga aaaccagata gacaatttcc
     2821 ggaagatgct gctcgccgtc gcaaaggacg tgcgagtcat tctcgtaaaa ttcgcggaca
     2881 ggcttcacaa cctgagaacg ctcgattatc tgcctcctga aaagcgcctg cggatcgccc
     2941 gggaaacgct ggacgtctac gctcccctcg cgcatcgctt cggtatggca cgcgttcgtc
     3001 gcgagcttga ggagcttgcg ttccgctatg ccgatcccga agcctgcgcc cgtcttgagg
     3061 cgcaggtcgc gcagcgcaaa gacgaatatt ggaatgcgat cgagcagttc tgcgaggaaa
     3121 tccgggaagg cttatcaaaa cgtggtatgc cggcggaagt cagcgggcgg gtcaaacacc
     3181 tgtacagcat ccacaacaaa atggtttcgg aagggaagga tctgtccgaa atctacgacc
     3241 tgctgggagt ccgcattatc gtcgaaactg ccgatcagtg ttacgacgcg ctcgcatatc
     3301 tccaccagca ctatcgcccg ctcgaagggc gattcaagga ctacatcggc aacccgaaat
     3361 cgaacggata ccagtcgctt cacacaaccg ttacgaacaa ccagcgaacg gtggtagaag
     3421 tccagatccg tacacgggaa atgcacgagg tggccgagga aggaattgcg cgccactggc
     3481 tgtacaaaga agggcgggct acgggggaaa tcgacgcgca gatgcgctgg gtccggcagt
     3541 tccttgagtg gtcgaaagat attcgggatc cgaatgagtt ctacgagcag ctcaaaatcg
     3601 acctctttcc ggacgaaata ttcgctctca ctcccaaagg tgatgtgata cctctgccga
     3661 agggtgcaac cgcactggat ttcgccttcg cggtccatac ggaggtgggc cttcatgcgt
     3721 ctggcgcgat cgtgggcggg cgaattgtac cgcttgggta cgagctccag acgtctgatt
     3781 cggtccgtat catcacgtcg gccaaccaga aaccgagccg cgactggctc aacctggtga
     3841 aaacagcgaa agcccgaaca agggtgaagc agtggcttcg ccaggcggaa ttcgcgctca
     3901 acgtcgaaat cggacgcgac cttgtggaga aacgtctgaa gaaggccggg ctcgtcctcg
     3961 cggaggaaga gtggggcgcg gttgcgtcgg cgttcaacac gaaggatcgc gagcatcttt
     4021 tcgccgcggt gggtgccggg gacctcgcgc cggaacgcgt ttttgaccac atacgttcga
     4081 tgatcgctcc ggcgccgaca aaagaagaag cgcctccaaa ggaactcccc gcaactgatt
     4141 cagaagcggg agggatcgtc ctgagcggaa tggataaccc gcttgtgcgg atcgcgcgat
     4201 gttgcaaccc tgtcccggga gatcccgtcc tcggtttcat cacccgggga cggggtttga
     4261 cggttcacca ccgcgagtgc cccaacatcc ggcagttgaa agaagacgcg ggacgcctca
     4321 taccagttcg gtgggaagcg aaggatgtca tcgggggcgc gggtcctttc aagacggcaa
     4381 tcatcgttaa aggttctgac agagtagggc tcctgagcaa cgtgacaagc acgattgcga
     4441 gcacgggcgc gaacatacgg cacgcggaaa ttcgcactct gcccgacggg gctcacagcg
     4501 cgttcctgct ggaagtgaag aacctcaagc agctcaaacg agtgatttcg aaactccgga
     4561 aagtgccgaa cgtggtagag gttcaccgac tggcgactga aggcgacgac gaagacgaac
     4621 tgcgcgcgat gatcgccgga agtccgttgc cgtttgtcga ggaatcaccg gacgacgagg
     4681 acgagtaacg tgttccgcct tacttcagaa accggcctgc caatgctccg ttcatcgctg
     4741 ctggacgagt tacccggcgt atggcacgga ttcacgacaa gaatcggcgg cgtgagtgcg
     4801 ggctcgttcg cgtttctcaa cctttcgttg aacgtcgggg attcccccga tgcggttctg
     4861 cagaacaggt cgttgctcgc aagatccgcc ggaatctcac cggaaatggt aaccctgcaa
     4921 gcgcaacagc atggctcaga cgtggtgttg tgcgatagtg cgagtcccga ggcggcgcac
     4981 cgtgctgacg ctttcatcgc tgcgcgcgaa gccataccgg tgctggtggg aaccgcggat
     5041 tgccttccgg tcctgctggc gtccgaggac ggacggattg tggcgtcaat tcacgcggga
     5101 tggaaaggcg tgctcgcgga agtaatcccg cgaaccgtca cggctatgca gacggtatca
     5161 ggcgcccgac cggaatccat gcgtgcggcg atcgggccgc acatccgcgc ctgctgtttc
     5221 gaggtgggag acgacgtcgc ccatcagttt cgaaaagaac atgtagttgc gccgcacgca
     5281 cgcggcggca agtcgtacgt tgatttgtcc gccgttgcgg tgtgtcagct agcggactca
     5341 ggcatcgcgg cgaaaaacat agacatctct tcaacgtgca cagtgtgccg ccacgatctg
     5401 tttttttccc accgtgccaa cggcggcact acgggacgga cggtcgcgat gatcatgcgg
     5461 cgcaggtaac gtgcactgaa tcgtacctgc cgtcctccat tttggagctt tccatgagcg
     5521 tttctgaagc cgctattgta ggtgtgatac agggaatgac ggagtttctt ccggtttcca
     5581 gttcggcgca catcgtgtac gcagagcatt tcctcggcat caaggaggga aatgtcgacc
     5641 ttgcgatcgt ggttcatttg ggaacccttg tcgcggtttt cgcggccatg tggtaccgca
     5701 tcgcaccgat catcagaggt acaatagcgg gggttgcgga tctggcagtt cggcgttccc
     5761 cgtgggcgcg agaggagttt cgccgggcaa cgtacataat tgccggaacg gttcccgcag
     5821 ctctgctggg ggtcctcttc gaggactcgc tgaaggaaac cttcggaaat cctttccaga
     5881 cctccgtgct gctcatagga acggggatta ttcttttcgc gacacgtttt gtcaggcgaa
     5941 acgaccgcga ttgggggcta tcacccgcac tggcagtggg gttcgcacag gcgctggcga
     6001 ttcttcccgg catatcgcgt tccggttcaa cgatttcaat gggtttgttt ctcagaattg
     6061 aacgcgaaaa ggcggcggag ttctcgttcc tgctttcaat ccctgtgatt cttggagggg
     6121 ccatcatcca cgtcgccggc atggtcggac aacccggcgg gctggctttg ttgagcgggc
     6181 cgctgattgc agggttctgt acttcggcca tttcgggtta cgcggctgtc cgagtcctgg
     6241 tgtcatttgt caagaaaggc gcgcttcact ggttcgcagt ctactgctgg atagtcgggg
     6301 cggcagggat gtggtatttc gcgtgaggac gggactatgc acaatcagtt cgcagacata
     6361 cgattcatga gcttcaacct ccgtttcccc tgtgagtccg acaccgggaa tctctgggac
     6421 gagaggcgtg cccgcgtgtt cgaaatgatc cagttgtacg atccagacgt gatcggcatg
     6481 caggaggtgt accggcgaca actgaatgac ctgctgagtg cgcttccggg ttacgcggca
     6541 gttgggaagg agcggtacgg gggaacggat gaggaacaca atccggtgtt tttcagacgc
     6601 gagaggttcc ttgcagaagg cagcaccaca aaatggttct ctgacacgcc ggacgtgccg
     6661 ggcacacgca cgttttgccc ttcggatcac cctcgaatcg ttacgtcggc gactctgatg
     6721 gatatcgcgg acgggtttcg cttttcggtt ctgaacactc atttcccgct gacaagggat
     6781 gaggacattc agcttgcgtg cgcacgagtc gtggaaagtc agttggcttt ggtacccaag
     6841 tctgcgccag ttgtattgat gggagatttc aacgctccac cagacggcgg ccttcacggt
     6901 ttttttacgc gttccggcgg cggcatcgga gaaggctttc gggacacgcg tgttcttgcg
     6961 caggaggttc ttggtccggc agggacggta cacggattca gaggcggcat gctcccggaa
     7021 agtcgtcgca ttgactgggt actgctcgcg ggagagggcc aggtcaccag gtatgagacg
     7081 gtcgggttcg aacgagacgg caaatacccg tcggaccatt tccccgtgtg cgttgacata
     7141 cgttttgcgc agccggaaaa ctgaaacgcc gaaaaggagt gcagagaaat ggaaagaccc
     7201 agagtgtacg tgcaccgcgt cggatcgtgg gctccccgtt atctggatga ccagaatcgt
     7261 tccagattgg cagatttcgc ggagatagcg gatgatgcct ccctagacgc accgcctccc
     7321 gacatcgcac atcgccttga aggggtgcag gcgatcctgt cgcttaacgg cgcgggcgca
     7381 gaggacatca ccgaagacgc cctgcgggcg gtcggaacgg ttcggatcgc ggtgatttcc
     7441 cactggttcc acgggtcgca cgatcgggca acggctgcgt ggagagccgc aggggtgcga
     7501 gttaccgatg catcatgggg aaataacttt gccgtagcgc aatggaccct tggcgccatg
     7561 atcaacggtg tcttccgcgt tgcagagctt gaccgcgcca tgcgtgccgg ggaagtctgg
     7621 cccgaccatc atttcacgag cagcatgctg gatggtcaga gaatcggcct tgtgggtctg
     7681 ggccgcattg ggaggctggt cgcgggattc ctgcagccgt ttcatgtgga aatcgtgggt
     7741 tatgacaaat acgtcaccgc ggaggaggca tcgaagttca acgtccgctg gtgtcctctg
     7801 gaacaggtaa tggcaacctc cgacatcata agcctgcatc tgccggttac gccggaaacg
     7861 acgcgactga taacaagagc ccacattgaa tccattcgga agggcgcgct tctggtcaac
     7921 tcggcgagaa ctgcgattct ggattacgaa gcgttccgtg agtgtctgca gaagggcctg
     7981 ttccgcgcta tcgtggacgt gtttgaaccg gaaccgcccc ctgttgacga cccattgcgc
     8041 accctgccga acgtggtaat gaccccgcac atcgcaggtt caacggctcg gatgtgccat
     8101 gtgtgcgggc gaacggccat tgaggagttg cggaagcaac tcgtgggcca gtcttcaggg
     8161 tgatggcaac ctgatcacgt tgccgtgagc tgaccatcag gcgtacctga acgacgtctg
     8221 gagaagggtg tttgctgatg tcgcaccgaa ttccggtgtt caaaaaggca ccgcgtgcca
     8281 cgataccctg ggagcacgat ccaacaactg gtcgaacgcc acaggcacgg agcaagagtg
     8341 ttctgaccgg aattggaatc ggtctcagtg tactgctggt gtgcctcaaa gggtttgcag
     8401 gatggcgatc cggaagcacc gcggtgatct cggatgcatt caactcggtc ctcgacatca
     8461 tttcgtacag cgccctgtac ctcagcgtcc gtttgcaggc gaaaccggcg gacctgacgc
     8521 accattatgg ccatcaccgg gcagaacctc ttgccggctt cctgatcgca gtatttgcgg
     8581 caatacttgg gggaatggtg ctccgggact ccgtcctgcg ccttttgagg ccggatccgg
     8641 ttcgaatgag cgccttaagc gtgattatcg ttctgtttgc catcgtcagc aaagtgatta
     8701 ttgccatcgc gtaccgacgc ggcgcgaagg gagggagccc cgcgatggat gcggcggcaa
     8761 tagactcccg gaacgacgca atggcgtctt cggtggcact gatcggcctt atcggaggcg
     8821 cggtatggcc ttacctcgat ggagcggcgg gccttgcgat cggcgcatgg attctgggca
     8881 gcggcgtccg ggtggggctt gagaacatag ggtttctgct tggaaaggca ccatcgaaag
     8941 cggttctgga ggagttgcgc gcggtggcag aaacggtacc gggattgatc ggaaccaaca
     9001 acatccgagc acattacgtg ggcaactcga tgcacgtcga ggtgcacatc gaggttgacg
     9061 caaacctcag catacgagag gcccacgatc tcgcgaaaac ggtaggtcag cgattggaac
     9121 gtctggacga cgtgtacgcg gcgtttgtcc atacggaccc cgtggatcgc gggctgaaga
     9181 atccgcagca aagcgaacag gcgtcttagc acctgaacgc agtcggaacc cggtactggg
     9241 ttatcgcagc tcttcgtatc ctttctcgac cgcaagatag atcagggaac cgatatcgtc
     9301 ggcgcggtca agggcacaca ccagaactgc gtcagcgttc gccacgatgg cgcagttctc
     9361 catcccgatc gcagcaacaa gtttgcccga accaaactgg acggtgttcg cgcaatccag
     9421 cattctgacg tcgccaatcg aaacgttgcc gcccgcatcg cgcgggagca aggaaaagag
     9481 cgacgcccac gtccggagtt cgacccgttc gatcgcagtg ggtaccacat aacaattggc
     9541 ggagtgttcg agtaccgccc ggtcgatgct gtttgccgga atgccggaat aggcttctct
     9601 gaacgcatcg tgatcgggaa gggaccttat tgccgctcgc actgcccgaa gaatattggg
     9661 cgcgtagaga tccatttccg cgtgcatagt ttccacgctg caggaaaaca tgccgctgtt
     9721 ccagtagcag tgcttctggg acaggtagcg ccgagctgtc tctgcaggcg gcttctcgtg
     9781 gaagcgtaac accctcacgg ttgccgccag ttcgctttcc tccggttccg gagcgccgat
     9841 tatgtatccg tagtgagggt tcggcccccc cggcgtcagt ccgaacatga cgagcgcatg
     9901 accgcgctcg gcaacttcgt gtccccggac aactgccgcg tgtaaggcct cgggattcct
     9961 gacccagtga tccgcgccca cgactgtcat ggttcctttc gcgttccgcg cggcgattgc
    10021 cgcggcagcc caggcaacgg ctgccgcggt gcccctcgca caaggctcgg aaaggacgtt
    10081 cgcttcggga acatcgggca actgttctct caccagcccc gcttgagctg agttcgtcac
    10141 caccaggatc cggtccggag gtgtgagcgg ggtaatgagg tcgaagctgt gctgcaggag
    10201 tgttctggga ccaaagaacg gaagaaactg cttgggtttc tgtgaacggc tctgtggcca
    10261 gaaacgtgtt cctcctccgc cagcaagaat gacggaatag gcattttccg gaagcagcac
    10321 gttcactgaa tcaaccttcc atgcagagta tgagtgcgcc tgaacgcacg ctgaagcacg
    10381 ttggcgtgct tcagatcatg cagacagccc cgtgtgaact ttcctgatgg ctttgacgat
    10441 gtcgtcgcag tcatcgtcgg tgagcaatgg agagacgtaa agccatactg agcggtcgag
    10501 aatctcccgg gattgcaggt agtcttcctt cgcgtaggtt atcggaccct cgtaaagcgg
    10561 gcagtcgaac gggcatgcat gttttgtcgg catgcgctga tgaagtacct gtggattctc
    10621 gaaaaccatc gtcccaccgt acaacacgaa gttcgggata ccctctttct caagcgcttc
    10681 ccgccatttt gcggccagat cagcagtttc gaggaagaac gtgatgctct gtccagtagc
    10741 gccttcgaca tcggccagag ttctaagtgt gaggccgggc agctcttcaa ctgctttccg
    10801 aatccgcctg ttgtggcgcc gcatggctgc caggttgctc tccagttttc ggagctgggc
    10861 aagggcgaac gcaccgtgca tttcgctcat acggtacact tccgcgcaga aattcttctc
    10921 cgcaacgttg ttccaacgat tgatcccgtg gtcgtgtgcc cgtaccgccc tgtcgaacaa
    10981 ctcttcgtcg ttggtcgtta ccacaccacc ttcacctgcg gtgattacct tctggaattg
    11041 caggctgtac gcattgacat cccccatgga acctacgcga caacctttgt aggttgcccc
    11101 ggcagactga gccgaatcct ctatgatctt caccccatgt ttctttccga tctccagaat
    11161 gggatccatg tcagccgcgc cacccagaat atgcaccggc attattgccg cagttctctt
    11221 cgtgatcttg cgttccacat cggccggatc gagaccgata tcggtaccca cattcgcaaa
    11281 aaccggccgc gccctcgaca tgacaaccgc accggcgcac gctatgaatg tgactgccgg
    11341 cactatcacc tcatcgccgg gcccgacgcc ggctgccgcg agcgcaactt tgagggctgc
    11401 ggttccggag gtgaccccga gcgcgtgctt gacgcccatg aattcctcga actccgcttc
    11461 gaactgctcc gttttcttcc tgaggtcagg accgtaaaaa cggaacggtg aatgggcgtc
    11521 gagtacctcc agcacctgct ccttttcctc ttctccaatg atacattcac cgggattcgc
    11581 gggaggcatg ggtttcgttc ggaccggtgt tccgccatgg atcgccagtt tttctgacat
    11641 ttgtgactcg ctttcgtttc agtttggaaa catttgacat ttacacacaa gataccgtaa
    11701 aaggataccg tctatggtgg cgaatagccg agaagctgca ttgccgcgcc aacgagaaca
    11761 aacgagccgc atacaaccca cgcgctgtct gatccggcat ccttcagtct gtccacagca
    11821 gctgagatgg tttctgcccg ttcgacacgc gtgtgagccc gcgaccacgc atcgtacatc
    11881 ctttccatgg acatcgctct aggtccagac ataggaacga taaccacgtt cccggccacg
    11941 ggcgccagga cctgcgcgat tccttcgacc tgtttgtcgg atgcgcaact gaagaccagg
    12001 gtcacctgct tgcccgcgaa cgtttgacga atcccctccg caaggcacgc agccgattca
    12061 ggcgtgtggg ccacgtccag catacaggga gggcgtgtcg gcagcacctg catgcgcccg
    12121 ggaataatcg tttcggacgc tacctttcga acaacagcct cagcgggcac acaaccgatt
    12181 tcgtcaagtg ccgccagagc ggtggcgaaa ttgtggcgtt gatggacgcc ctgcatcttc
    12241 agagccaccc cgtcgacaga tcgtgccttc aaccgaaccg cccagggttc ccctgtttca
    12301 tcaaacgtaa tgtcgcgtcc gacccaggta ctcggcgcat gctttcccgc cgccacgcgc
    12361 gcaatcacgg cggccgcttc gggcttctgc tccgccacca caacggggac ccgctcctta
    12421 atgatcccgg ctttctccgc ggcaatcgcc tctaacgtcg ggcccagaaa gcgcgtatgc
    12481 tccagcgcaa tcggtgttat cacggagact ttcggaagga gtacgttcgt cgcgtcgagg
    12541 cggccgccca tacccacctc gatgacggca atttcgactt cgctccgacg gaatgcttca
    12601 aaaccgatgg cagtgaggag atcgaagaac gagagacggt cgtccgggtg atctgcctgc
    12661 catctggcaa cgtgcggctg gatttccgtg gtgatgtccg caaacaatga ctcgctgatt
    12721 ctggcgccgt tgactcgaat ccgctcacga aaatccacca ggtggggcga ggtgaacagc
    12781 ccggttctga ttccagaagc acggagaagg gcctcacaat agatcgcgac ggagcctttg
    12841 cctttggtgc ccgcgatgtg cacggagtcg aacgaccgga acggttcccg catggtcgcc
    12901 atgagcgatt gaacgcggtc gagcttgaag gcggttgcgt acgaaaaatc gggggacttc
    12961 tcgtagtcaa tcagtgcatt gaggtaacgc gttgcttcag aaaacgtcat cggatcagag
    13021 tgtcatggtg agtgggaaca acggcaaaaa ccgaaagggc tcatccaaaa cctgcacgat
    13081 ctcggtgttt gggtcaacaa cctcccgacg ccgaatcgcg gcgagttttc tcgcacgagg
    13141 gctgccggtg cctgccgtat tccccgtgga catgttccgc ggaaggaaac ggaccacacc
    13201 ccgatgccaa cggcagacag aggcttgctt acggcttcca ctcccggcgc cgcaagatca
    13261 ggccctgaag agttcgccaa ggcgtttacc gaggaatctt ccggcgacaa tcagactcgt
    13321 ggcgaggagc accattccga gcaatcccat cgcgctggca acgtatggcc catccgcaac
    13381 acggagactc atcgcgtaga tggcctttgt aatcggatag aactcctctt tcgccgcgag
    13441 aatcaggctg tctgagacct ccagcatggc gaacgagaag gcgagcaccg acccggcgac
    13501 gatgttggcg aacacgagcg ggagcgtgat tcgtctcaac gttcggagcg gtcccgcacc
    13561 gaggttcagc gacgcctctt ccagagtttc gctcgtttgc tgaagtcccg cgtacgcagc
    13621 ccgcaccatg taagggagtc gccgaattgc gtacgcgatg atgagaagag gcatggggtt
    13681 tcgtttgatg tccagaaacg agccactgaa tgcggcgaca tagccgaagg cgagcaccag
    13741 cccgggcaag gcaagcggca acatggccag cccgtccaaa acgtcccggc cgatgaagcg
    13801 cttcctcgcg aggaaatagg caatcgtcac gccgagggcc acatccacca gagtgctgag
    13861 cgaactgaga aacaggctgt tcctaatact cgacagagta aggggatggg tgaatacgcg
    13921 cccgatgtac acggtggtgt aactggaagg cagaatggtc atgaaccacg tatcggctat
    13981 ggcggaaaga accacgcttg cgtggggaac aagggcacat ccgatgacgg ctgcgatcga
    14041 gacccatacc acaggcaccc atcgccgggg aagcggggaa gacgacccct ggacgtggcc
    14101 ccgagccatc atctggtaat tgctcgatcc aagcaccttc ttgcttatcg cgaagaatac
    14161 gac
//
LOCUS       DAOTNG010000038        14086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4239_length_14086_cov_5.444159, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000038 DAOTNG010000000
VERSION     DAOTNG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_14086_cov_5.444159"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="P5179_03075"
     CDS             <1..1463
                     /locus_tag="P5179_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94235.1"
                     /translation="PQSEPTDIIRVTTDQRAHEATSVSVANLTGETISVRVWLDPWRG
                     GETAPPLSAVALRRRVFVATARGGYTPDALPELDPSGVVTIPAGETLRLWLDWSSGDT
                     PAGVYSSTLSVRALTVAGDVLRIPLQWEVSTVALPDSMPLAFRVWAYEQPAFFERDAV
                     WADLRAHYVNVYDIPVPTVRYNADGEIAAEDWAAVEDVLRRAPSGSFFLWHGGETIVQ
                     PAEGAPGSGSPAWEKAFQTFVPRWLDGLAARGVLPDRHANYILDEPGIDGGPSVYELI
                     RIARLYKTADPRVRIFANPAGGATAQHVDDLNEVVDIFDPIMNFAHLERVLAKGKPTW
                     TYECGDGAKDNTRMRYYWQPIWQGTEMGLSGLGIWSYAGRSVDFWQGPHDGCCDWELV
                     YHGAGGSVIPSHRWQGVRIGIEDHARLWLLSRAADNARGRGDTARAMELTRSRAAMIR
                     QVLESGCDEGVVTRVRGQIRGILAGEIGQKRTERND"
     gene            1547..2779
                     /locus_tag="P5179_03080"
     CDS             1547..2779
                     /locus_tag="P5179_03080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS94236.1"
                     /translation="MTGKVIDRVVFMPSPCKGVATHASSYYTTREGGNLMSIHGYESR
                     SDTTDVAFVRFSDDNGRTWSAEETFQTEFPDPKGTRRRYPAGGYVDPLTGRYLTVRNE
                     GVLPTDDPLEGMRQWKLHYSVSEDGGRTFLVDEQIIHDGEEYDAVNHLPGISVGKTCA
                     MIGDMGQRPITRADGVLLVPIQYTPAGPDGMYLNPGAGFTYTNCMVLFGHWLPGGRLR
                     WTASDKIIADPERTTRGLIEPTIAQLPDGRIIMVMRGSNDKRPDLPGWKWYSESRDGG
                     QTWSVPAPWTYDDGTTFHSPSSCSQLYRHRDERLFWIGNICEENPQGNSPRYPLVFGQ
                     VDEVTGRLLRRSVWAVDDRKPDESPFLTLSNFYAREDRETGHVLIHMTRLFARDFREG
                     GSIDWTADSLQYRIELED"
     gene            complement(2836..3651)
                     /locus_tag="P5179_03085"
     CDS             complement(2836..3651)
                     /locus_tag="P5179_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS94237.1"
                     /translation="MNRGAVTSVSMLLAALAFLSLSCSRGPKAGDVIKVKGKEMVYIP
                     GGTVTLGDSTMGRLTVTLKPYLIDKHEVTNGEYREFAKVFGLPFSVAPAYDDLPVVNI
                     PWETADAFAAWRGCRLPTEAEWEFAARGTDGRRYPWGNSADAEKANGDDGVDIMTVPD
                     GSKDGFSGLAPVGMFPKYASPFGVEDMSGNVWEWVQDWYAPYPPNAVSDYAGPDSGEQ
                     KVVRGGSFKTNFLNLRTFHRAYRVPGAAADDVGFRCASDYPPREPGALPSNVR"
     gene            complement(3786..4055)
                     /locus_tag="P5179_03090"
     CDS             complement(3786..4055)
                     /locus_tag="P5179_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94238.1"
                     /translation="MVRGVAAMLSSRDVLDVLDHLRKGLRGLRDAAEIDRDPSLQKAI
                     NALQSQLSGVISSVEAVARRSGIIPKETILPRPSHGENREESPST"
     gene            complement(4179..5147)
                     /locus_tag="P5179_03095"
     CDS             complement(4179..5147)
                     /locus_tag="P5179_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRS94239.1"
                     /translation="MSKPIALQRVRGIVVYRNGALGDTIVTFPVLQNLRLAFPHVPLH
                     FVASKEVGLLAQTCGLTDSVVSADSEWARKWFAGDAAGMRALVEGADALIAFTSEDDE
                     LKETARRAGFGFVRFQSPALPANTTIHIVDHLLRALNGLIDGPLERIPSIRLPREQVV
                     PARRILDESGLRSGTPYVFIHTGASSRQRVWPYAVDFARLLREKTGCTPVLHMGPIEK
                     ENAALEPHEVADMPILGPFTVLELAAALSCAAAYVGSDTGPSHLSAALGIPTFTVFGG
                     NDEGRKNAVVWSPRGQRAHALYAQEGQEWPSPEQVVQALILSGVLP"
     gene            5266..6171
                     /locus_tag="P5179_03100"
     CDS             5266..6171
                     /locus_tag="P5179_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS94240.1"
                     /translation="MKHENRVVPYAPGHLEALLKVVNAHVAALPPQEGVIDRTALETI
                     VNRPEALWAHHTREHSIDAALVRMVAISDDEVVGLAVAWHEIGGEHAHLPLLVFEPRA
                     PGAAKALLGRIFDELRDRGCTSVATDERNPLGIGWFGVPDSWPHVLETMHQEGFSVAD
                     GWVILTGRTDSIPVEPPRPANEMITCVFVNNENMGEWRLEFFHDDKLVGECETWAVPP
                     HLASCPGFHDWITVEWVGVEEAFRRRGLAFWLLVKQMHYHLRAGRTRVALWTGPGNRP
                     ARALYYRLGFSDGPETFVLARSLAL"
     gene            6331..8901
                     /gene="clpB"
                     /locus_tag="P5179_03105"
                     /pseudo
     CDS             6331..8901
                     /gene="clpB"
                     /locus_tag="P5179_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
     gene            complement(9082..9384)
                     /locus_tag="P5179_03110"
     CDS             complement(9082..9384)
                     /locus_tag="P5179_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94241.1"
                     /translation="MKIDAEERVIRYLRHVLQGHPRSGQQYVDGLVAKGMTHAEVVSS
                     VLIPARARIADLRKAHYVNAIDAKNALSVTNQAIARLSPAQKAYRVTSPTAVSAAM"
     gene            complement(9580..10437)
                     /locus_tag="P5179_03115"
     CDS             complement(9580..10437)
                     /locus_tag="P5179_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRS94242.1"
                     /translation="MTTNTAGGSKLDDQALEAYLREVRDYVLLTREEEVQLARQIKRG
                     DQKALEKLTSSNLRFVISVAKKYQNRGMSLLDLISEGNVGLMEAAYRFDETKGFKFIS
                     YAVWWIKQAILKALSEQSRIVRVPLNRVGDAQKVDKRVSALCQYYGREPTIEEIASSA
                     EMSPEDVEKALDISRPHLSLDDPGHQEGGRTLLDTIPLEDEAFQESPFERRFGIGVEE
                     TLSELDEREAEIIRRYFGLDNQDPESLKDIGASFGITRERARQLKERALAQLRKTHKK
                     VNMESRRVV"
     gene            10807..11502
                     /gene="queC"
                     /locus_tag="P5179_03120"
     CDS             10807..11502
                     /gene="queC"
                     /locus_tag="P5179_03120"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRS94243.1"
                     /translation="MNKPRAIVLASGGMDSTVTVALANEQGVELALLHVNYGQLTERR
                     ELKAFHAVADHYRVPQSLRLVVDIGYLVQIGGSALTDATIPFPEPTFSDRRMIPSTYV
                     PQRNGNLLFIAAAWAEVIGAESIWAGLVEEDSSGYPDCRRSFCDAIERAIAEGNHDQH
                     SDPRIITPLIGLSKADIVRTGVRLAAPFEKTWSCYRREDTACGECDSCRLRLRGFAQA
                     GVRDLIRYADPGV"
     gene            complement(11511..12770)
                     /locus_tag="P5179_03125"
     CDS             complement(11511..12770)
                     /locus_tag="P5179_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS94244.1"
                     /translation="MDTSFQQGLPEGYPRSVRRPGDRQEFARRVGLIGQSPAIQQVLI
                     SIAQIAPTSTTVLITGETGTGKEIVARAIHELSPRRREPFVALNISALNEGTVESELF
                     GHEKGSFTDAVREKKGIFEAAGKGTVFLDEIGDISPALQVRLLRVLEEREFKRVGGIE
                     PLRAEARVITATNRDLAELVNAGTFRADLYWRLHVFEIHLPPLRERKEDIPLLVEHFV
                     ERFCSENNRAVPVISKEAMEYLLSYDWPGNVRELRTVVERVLVLSDVPIIRPENLPDM
                     VRDKVFANRLLPVASRGSGAEKMELGIIYRTLLELRADMADLKQILTAPGNGVASRSW
                     SSASLPPQDASSPASKPHVVDIEVSEDDTPSGETTPARTLTLRDLERDAIRQALAATK
                     GNRREAARSLGIGERTLYRKLREYNLS"
     gene            complement(12793..13479)
                     /locus_tag="P5179_03130"
     CDS             complement(12793..13479)
                     /locus_tag="P5179_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS94245.1"
                     /translation="MDAPGSYLRNVPFFAGLSESELAQIGTLVTQRVYPPENLIILAE
                     DEGEALFTILSGQVKVSILNEDGKEVILAVLGQGDFFGEMSLLDGKPRSANVIATEET
                     TVLILRRRDFLRLVERSPHMAIKVVAALTARLRKADRKIESLALMDVSGRVASVILQI
                     AEERGVSSQRGICIHNPPNQQAIANMAGTSRETVSRVMKRLKERGYVAMRGKDILITR
                     EADLRADYSL"
     gene            complement(13492..>14086)
                     /locus_tag="P5179_03135"
     CDS             complement(13492..>14086)
                     /locus_tag="P5179_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94246.1"
                     /translation="QAADAYNRMLEQDTTSQFAAQAMWNLILSYEQIQRLDSAVRTMR
                     VFLSKFPDSPHVVRVWVKIGDDLNRLGEFPAAVAAYRQALDNVAGTPEEPDARFGLGE
                     AHFNAGQYREAVIEWLKLAYHSQSGSRWAVTALFRAAKANEKLGQLEDAKMLYRKIIA
                     MEGESDLGRAAALQLMSISGQNLDSGSESGSSPTDTR"
BASE COUNT         2981 a         4154 c         4104 g         2847 t
ORIGIN      
        1 ctcctcaatc cgaacccact gatatcatac gggtaacgac agaccaacgc gcgcacgaag
       61 cgacctcggt ttccgtcgcc aaccttaccg gcgagacgat ttcggtgcgc gtatggttgg
      121 atccctggag ggggggcgaa acagcccctc cactcagcgc ggttgctctt cgccgcaggg
      181 tgttcgtcgc gacggcccgg ggtgggtaca cccctgacgc gttacccgag ctcgatcctt
      241 ccggcgtggt gaccatccct gcaggcgaaa cgctccgatt gtggctcgac tggtcatcgg
      301 gcgacacacc cgcgggagtc tattcctcaa cgctttccgt gcgtgcgctg actgtcgcag
      361 gggacgtact gcgtatcccg ctccaatggg aagtgagcac cgtcgccctc ccggattcca
      421 tgccactggc tttccgcgtc tgggcatatg aacaacccgc tttctttgaa agggacgcag
      481 tctgggctga cctccgggcc cattacgtga atgtgtacga tatcccggtg cccaccgtgc
      541 gttacaacgc tgacggggaa atagccgcag aggactgggc cgccgtggag gatgttcttc
      601 ggcgcgcccc gagcggctcg ttcttcctgt ggcacggtgg cgaaaccatc gtccaaccgg
      661 cggaaggtgc cccgggctcg ggttctcctg cgtgggaaaa agcgttccag accttcgtgc
      721 cacggtggtt ggacggtctg gccgcgcgcg gggtgctgcc agatcgccac gcaaactaca
      781 tcctcgacga gcccggtatc gacggcggcc ccagcgtgta cgaactgata cgcattgccc
      841 gcctgtacaa aacagccgat ccccgggtga ggattttcgc caacccggcg ggaggcgcga
      901 ccgcgcagca tgttgacgat ttgaacgaag tcgttgacat cttcgatccc atcatgaact
      961 tcgctcatct cgaacgggtt ctcgccaaag gcaagcccac gtggacctat gagtgcggcg
     1021 atggcgcgaa ggataacact cggatgcgct actactggca gccaatctgg caaggcacag
     1081 agatgggcct ttctggtctg ggcatctggt cgtatgccgg gaggagcgtc gatttctggc
     1141 agggccccca cgacggctgc tgtgattggg agctcgtgta ccacggcgcg ggcggttctg
     1201 tgataccgag tcatcgctgg cagggagtgc ggattggaat tgaggaccac gcgcgcctgt
     1261 ggttgctttc gcgggcggcg gacaatgcgc gcgggcgagg tgataccgca cgcgccatgg
     1321 aactcacacg cagcagagca gcgatgatac gacaggttct ggaatccggg tgcgatgaag
     1381 gggttgtaac gcgcgtcaga ggtcaaattc gcgggattct tgccggagaa atcggccaaa
     1441 aaagaaccga acgaaacgat tagtgctcgt cggcaacaat ctgtgaagcc atcaaagccc
     1501 gtaacgaagt gctgcaggcg gacaattgag cagggggttc atgcgcatga ccggaaaagt
     1561 gatcgacaga gtcgtcttca tgcccagtcc gtgcaaagga gttgcaacgc acgcatcctc
     1621 gtattacacg actcgtgaag gtggtaacct catgagcatt cacgggtacg aaagtcgaag
     1681 tgacaccacg gacgtggcgt ttgttcgatt ctcggacgac aacggtagaa cgtggagcgc
     1741 tgaagaaacg tttcagaccg aattccccga cccgaaagga acgcgacgca ggtaccctgc
     1801 gggagggtac gtggatccgc tcacgggaag gtacctgacc gtgcgtaacg agggagtgct
     1861 gccgacggat gatccccttg aagggatgcg tcagtggaaa ctccactact cggtttcaga
     1921 agatggtggc aggactttcc ttgttgacga gcagattatc catgatggag aagagtacga
     1981 cgcagtcaac cacctcccgg ggatttcggt cgggaagaca tgtgcgatga tcggagatat
     2041 gggacagaga ccgatcacac gtgcggatgg ggttctcctc gttcccatcc agtacacccc
     2101 cgccgggccg gatggcatgt atctgaatcc cggcgcgggg ttcacctaca cgaactgcat
     2161 ggtcttgttc gggcattggc ttccgggagg gcgcctccgg tggacggcgt ctgacaagat
     2221 aattgcggat ccggaacgaa caaccagggg actcatcgaa ccaacgatcg cgcagcttcc
     2281 cgacggccgc attatcatgg tgatgagggg cagcaacgac aagcgtccgg acttgcctgg
     2341 gtggaaatgg tactcggaat cccgtgatgg cgggcaaacg tggagtgttc ccgctccgtg
     2401 gacatatgac gatgggacca cgttccattc accgagttcc tgttcgcagc tataccgaca
     2461 ccgcgacgaa aggttgttct ggatcgggaa catctgcgag gaaaacccgc agggaaacag
     2521 cccgcggtac ccactcgtct ttggtcaggt ggacgaagtg acaggacgct tactacggag
     2581 aagcgtgtgg gcagttgacg accgcaagcc tgacgaaagt cccttcctta cgctatccaa
     2641 cttctatgcg cgggaggaca gggagacggg tcatgtgctg atacacatga cgcgcctctt
     2701 cgccagggat ttccgggagg gaggctccat cgactggacc gccgacagcc ttcagtatcg
     2761 aatcgaactc gaagactgag tgacgcggcg taacgacggc ggggcagacg gctctgcccc
     2821 gccaaatgcg gcaattcacc gtacgttgga aggcaacgcg cccggctcac gcggagggta
     2881 gtcgcttgcg caccggaacc ctacatcatc cgccgcggct ccgggaacgc ggtatgcccg
     2941 atggaacgtg cggagattga ggaaattcgt cttgaagctc cccccgcgca cgaccttttg
     3001 ctcgccgctg tcggggccag cgtagtcaga aaccgcattt ggcggatatg gcgcatacca
     3061 atcctgaacc cattcccaga cgttcccgct catatcctcg acgccaaacg ggcttgcgta
     3121 tttcgggaac attcccaccg gtgcaagacc tgaaaacccg tccttggaac catcgggaac
     3181 agtcatgatg tcgacgccgt cgtcgccgtt ggccttttcc gcgtcagccg agttgcccca
     3241 ggggtaccgc cttccgtcag ttccccgcgc cgcgaactcc cattcggctt cggtcggcag
     3301 gcgacatccc ctccacgccg caaacgcatc ggcggtctcc cacggtatgt tcacaacagg
     3361 gaggtcgtcg tacgccggag cgacagagaa cggtaagccg aataccttcg cgaattcgcg
     3421 gtactccccg ttcgttacct cgtgcttgtc gataagatag ggcttcagcg taaccgtcaa
     3481 ccggcccatt gtcgaatcgc cgagcgtcac ggttccgccc gggatgtaca ccatttcctt
     3541 gcccttgacc ttgatcacat ctcccgcttt ggggccgcgg ctgcacgaaa gggacaagaa
     3601 tgccaacgct gcaagaagca ttgagaccga cgtaacggcg ccacgattca tgttcgctct
     3661 ccctattcca ccccggcgga gggggcgtca cctgtcagaa tcacgaaaac gagggcaaaa
     3721 gcgtcgctct cgccctcgtt tgtagtatcg caagaacacc gtcaggatca aacgggacgc
     3781 ggtattcagg tggacggtga ctcctcacga ttctcgccgt ggcttggcct cggcaggatc
     3841 gtttccttgg gaatgattcc gcttcgcctc gccaccgcct ccactgagga gatcacgccc
     3901 gacaactgac tttgaagtgc attgatagcc ttctgcagag acggatcccg gtcgatctcc
     3961 gcggcatcgc gcaatccgcg gagccctttc ctcaggtggt ccagaacgtc gagaacatcc
     4021 cgtgatgata acatggccgc cacgcccctt accattgaac tttcctgttg caatgtcccg
     4081 aattctctgt aacaaaatgt acaaccggtt tgctcaggcg gagttaacgg cgcatgagta
     4141 gctcatgaaa ctttgttcat gtacgccgat gtatgggttc atgggagcac tcccgacagg
     4201 atcagtgcct gcaccacctg ttccggcgaa ggccattcct gcccttcctg agcatataat
     4261 gcgtgcgcgc gttgcccccg gggagaccat actacggcgt tctttctccc ctcatcattg
     4321 ccaccgaata ccgtaaacgt cggaatacca agagcggccg agagatggct ggggcccgtg
     4381 tcactgccga catatgccgc cgcgcacgac aacgccgctg ccagctcgag taccgtgaaa
     4441 ggcccaagga tgggcatatc agcgacttcg tgtggttcaa gcgcggcgtt ttccttctca
     4501 attggtccca tgtgcaacac cggtgtgcaa cccgttttct cccgcaacaa tctggcgaaa
     4561 tccacggcat agggccacac tcgctgacgg gaggatgcgc cggtgtggat gaagacatat
     4621 ggcgtaccgg accgaaggcc ggattcatcc aggattctcc ttgccggtac tacttgttcc
     4681 cggggcagac gtattgacgg tattcgctcc agcggcccgt caatcagccc gttcagagcc
     4741 ctgaggaggt gatcgacaat atggatggtc gtattcgcgg ggagtgcggg cgactgaaaa
     4801 cgaacaaatc cgaatccggc gcggcgcgcg gtttctttca gttcgtcatc ttccgaagtg
     4861 aacgcgatga gggcgtccgc accttccacc agcgcgcgca ttccggcggc gtcaccggca
     4921 aaccatttcc gggcccattc cgaatctgcc gataccaccg aatcggtcaa cccgcatgtt
     4981 tgcgcgagga gccccacttc ttttgaagcg acgaaatgga ggggtacatg gggaaaagca
     5041 agacgcaggt tctggagaac cggaaaagtg acaatagtgt ccccgagagc tccattcctg
     5101 tagacgacga ttcctctgac gcgctgaagg gcaattggct tggacattgg atgctccgaa
     5161 ggcttgattg cgctggttac gcaggaacgt acatttcatc cctgtcaggc cgagggagcc
     5221 cgacctgtca ggcaagaaga gaactggaaa tcccgacaag accgaatgaa acacgaaaac
     5281 cgcgtcgttc cgtatgcccc cggccacctc gaggcccttc taaaggttgt caatgcccac
     5341 gttgcagcat tgccaccaca ggagggtgtg attgacagaa ccgcgctgga gaccatcgtg
     5401 aaccgccccg aggcgctgtg ggcccaccac accagagagc acagcatcga tgctgctttg
     5461 gttcgaatgg tcgccatttc tgacgatgag gttgttggcc tcgcggtggc ctggcatgag
     5521 atcggcggcg agcacgctca tcttccgctc ctggtgtttg aaccacgcgc gccgggtgcg
     5581 gcgaaggcgc tcctcggtcg gatttttgac gagttgaggg atcgcgggtg caccagtgtc
     5641 gccacagacg aacgcaatcc tttgggaatc ggatggtttg gcgttcccga ttcctggccg
     5701 cacgtcctcg aaaccatgca ccaggagggg ttttctgttg ctgatgggtg ggtcattctg
     5761 accgggagga cggactccat tccggttgag ccaccgcggc ccgcgaacga aatgattacg
     5821 tgtgtgttcg tcaacaacga aaacatggga gaatggcgcc ttgaattctt ccacgatgac
     5881 aagctcgttg gcgagtgcga aacctgggcc gtgcctccgc acctcgcctc ctgccccggc
     5941 ttccacgact ggatcaccgt ggaatgggtt ggtgtggagg aagcgttccg gcgccgtggg
     6001 ctcgcgttct ggctactggt gaaacagatg cactatcacc tgcgcgcagg ccgcacacga
     6061 gtagcactct ggaccggccc cggcaaccgc cctgcgcgag cgttgtatta ccgcctggga
     6121 ttctcggacg gcccggagac gtttgttctg gctcgctccc tcgctttgta atgccaatgt
     6181 ggcaccccga acagcctcac tgcctatcgt tcctgcgagt gtgcgccatt atggcataaa
     6241 ttactgcccc attcctttgt ggtatccttc ttgcaaaagc aagccgaaaa aaccggacat
     6301 tcacgaagga gaaataacga tatggcagca atggacgcca accgaatgac tcaaagcacg
     6361 cagaacgcgc tggctgctgc tcagcagacg gcgctgaatc ggaaccacaa ccagttggaa
     6421 cccgagcaca tccttctggc gctgcttgag atggagcaga atctcccgca ggagctactg
     6481 aggcgaagcg gcgggaacat cgagaccatc acgcgcgaag tggtttcagc cctcgacagg
     6541 ctaccgcgtg tccaaggcga caacgtacag gtgtacgctt cgccgaacgt ccagcgagtc
     6601 ctttccgctg cgtggaaacg cgcgcaggaa ctaaaggacg aatacatttc tgtcgagcac
     6661 ctcctgctcg gaatactcga ccagaaacac acgcaagcgg cgaaaatcct cgaacgaaac
     6721 ggcatcaccc aggagaagct cctccaggcg atgaaggaaa tccgcgggag ccagagggtc
     6781 accgaccccg acgcagaatc tcgctaccag gcgctggaga agttcagccg tgacctcacc
     6841 gccgcggcgc gagaaggcaa acttgacccg gtgatcggga gagatgagga gattcgtcgg
     6901 gtcatcaagg tcctttcacg ccgccggaaa aataaccccg tcctgatcgg tgatccggga
     6961 gtggggaaaa cggctatcgt tgaagggctt gcgcaacgca ttgtcgccgg agaggttccc
     7021 gattccctcc aggggcgtcg ggtagtttcg ctggacctgg gttccatgat cgcgggatcg
     7081 aaattccgtg gcgaattcga ggaacgcttc aaagcagtgc tccaggaaat tgaagcggcg
     7141 gaaggtcaga tcatcctctt catcgacgag ttgcacacag ttgtgcgggc gggagccgcg
     7201 gaaggcgcga tggatgcggg aaacatgctc aaacccgcgc tggctcgtgg cgtcctccgt
     7261 tgcgtgggcg cgacaacgct cgacgagtac cgcaagcaca tcgagaagga cgcggcactt
     7321 gaaaggcggt ttgccccggt ctacgtcggc gaaccgtcgg tggaagagac gatttcgatt
     7381 ctgcggggac ttcgcgaacg gtacgaggta caccatggcg tgcgaatcaa agacgaagcg
     7441 ctggtggctg ccgcgcgcct gtctcacagg tacatatcag accggttcct gccggacaag
     7501 gcaatcgatc ttgtggacga agccgcggca gaattgcgga ttcagttgga tagcatgccg
     7561 cctgaactcg aagccattga acagcgcatt gtccagctgg aaatcgagcg ctctgccctc
     7621 aagcgcgagg aagacgccga ggagcgcctt gcccctgtcg aaaaggaact ggcgaatttg
     7681 caggagaaac gtgcggcgct gaaatctcac tggctcaaag agaaagagat cgtcaacgcg
     7741 ctcaaaaaac tgaaggagca ggcggaagag gctcggacag agctcgaccg tgcacgcaga
     7801 acaggacaac ttgagctggc gggacgaatc cagtacagcg aactcccccg catccaggcg
     7861 gaaattgcac aggcgcagga cgagctggca cgtgtccaaa aagaccagaa gatgctgcac
     7921 gaagaggtcg aagaggagca catcgccgga atcgtcagca agtggacggg aatccccgta
     7981 agccggctgg tagaaggcga aatggaaaag ctcctccaca tggaagagcg gcttcacgaa
     8041 cgtgtcgtgg ggcaggacga agctgtgacg gccgtcgcga acgcggtgcg tcgtgcacgg
     8101 gcggggttga gtgaccagaa ccggcccatc ggttcattcc tgttcctggg tcccacggga
     8161 gtcggcaaga ccgaactggc aaaagccctg gcggagttcc ttttcgacga cgaacgggcg
     8221 atggttcggc tggacatgtc tgagtacatg gaacgccaca gcgtgagccg ccttgtggga
     8281 gcgcccccgg gttacgtcgg gtacgaggaa gggggccaac tcacggaggc cgtccgccga
     8341 cggccgtatc aggtcgtgct ccttgacgag atcgaaaagg cgcatcatga cgtgttcaac
     8401 gccctgttgc aggtgctgga cgacggccgg ctcactgatg gtcagggccg gacggttgat
     8461 ttcagaaata ccgtccttat catgacgtcc aacgttggaa gccagaactt cgcgttcggg
     8521 gagaccgccg attacacgag aatgcgcgcg caggtgatcg aggctctgaa agccgaattc
     8581 aggcctgagt tcatcaaccg cgtggacgag acgatcgtgt tccacgggct cgaaaagaaa
     8641 cacatccggg agatagtacg cctccaaacc agactgctcg cccacaggat cttcgagcag
     8701 gcaagggtca cgctgacact gacagacgcc gccagcgacc tgctggggga actcgggtac
     8761 gacccggcgt acggtgcgcg cccgttgaaa cgcgtcatcc agcgtcagct cgaaaacccg
     8821 ctggcgatcg cgcttctgga acggcgcatc aaggaaggcg atatcgtaag cgtcgacgtc
     8881 cacgacggcg cgctcgtgtt ccgtaccaca aagccggatg gatccgctgc acccggcgcc
     8941 gagtccgtga cttcgacggc ggaatcggtc gaggacgttg aggatgcgga gtttgaggag
     9001 ctgaaataac ctctgagtgg gaaaagaaag agccggcggg gttccgcgga accctgccgg
     9061 ctctttttgt tgagcaggga attacattgc cgcagaaaca gcagtcggtg acgtgactct
     9121 gtacgctttc tgcgcaggcg aaagacgcgc gatcgcttga tttgtcacgg acaacgcgtt
     9181 tttcgcatca atggcgttga catagtgcgc tttccgcagg tcggcgatcc gagcacgggc
     9241 gggaatcagg acgctggaca caacttcggc gtgcgtcata cctttcgcga ccagtccgtc
     9301 aacgtactgc tggccgcttc gcggatgtcc ctgcagcacg tgccgcagat accgtatgac
     9361 tctctcctca gcatcgattt tcatggtaat ctcctcttcg gtgcgctgtt caattcgatt
     9421 gtcgattttc atatcagtgt gtattgtggt acgcccgggg aacatcccaa caaacaagcc
     9481 tttctgcacg aaacgcaaga agcctcccgc gcgttatggc accggaggct tcaaattgca
     9541 aataccttgc caaacttggc aggcgtgatt acgcgccgct caaaccacgc gtcggctttc
     9601 catgttgacc ttcttatgcg tctttcgaag ctgagcgagc gcacgttctt tgagctgtcg
     9661 ggcacgctcc cgcgttattc cgaaggaggc cccgatatct ttcagggact cagggtcctg
     9721 gttgtcgagc ccgaaatagc gtcgtatgat ctccgcttcc cgttcatcaa gttcggataa
     9781 cgtttcttcc accccgattc cgaaccgccg ctcgaacggg gattcctgaa atgcttcatc
     9841 ttccagaggg atcgtgtcca gcagagtgcg tcccccctcc tgatgccccg gatcgtccag
     9901 ggacagatgg ggccgggaga tatcgagcgc cttttcgaca tcctcgggtg acatttctgc
     9961 agagcttgcg atctcttcga tcgtcggctc tctcccgtaa tactgacaca gcgcacttac
    10021 tcgtttgtca accttctgcg catctccaac cctgttcagc ggcactcgga caatccgcga
    10081 ctgctccgaa agcgccttga gtattgcctg cttgatccac cacaccgcgt acgagatgaa
    10141 cttgaaccct ttggtttcgt caaatcggta cgctgcttcc atcagtccga cgttgccctc
    10201 gctgatgaga tcgagaagcg acatacccct gttctggtat tttttagcga cactgatcac
    10261 gaaacgaaga ttggaactgg taagcttctc cagcgccttc tggtccccac gcttgatctg
    10321 gcgcgcgagc tgtacctcct cctcacgcgt gagcagcacg taatcacgca cctcccggag
    10381 gtacgcttcc agggcctgat catcgagttt ggaaccgccg gcggtgttag ttgtcatagg
    10441 ctccgctccg tttgattgaa gttttttcca tttcgacaaa cagctacgtc agggggttcc
    10501 acgacgtggc cggagccgca aatgaatatt ctcgcaccac ttgcaggata aagaaaacgg
    10561 ttcccgcaag cgtcacgaga gatgatgcac aatatttgga atatactgca attcataatg
    10621 ttccgctaac gccggagact tcaatcctca caggcagcgc ggcgccaggc ctgcacagtt
    10681 cgggcgaacc gggattgtgt taccccccgg attttctctg cgcagtccat cgcaccgcag
    10741 ccttggccaa ggacgccatg caagctttca cctggtgtcg taaattttca aattggaggt
    10801 gactgtatga acaaaccgcg tgccatagtg ctcgcatccg gcgggatgga cagcacagtt
    10861 acggttgctc tggcgaacga gcagggtgtc gaactcgcgc tgctccacgt gaattatggt
    10921 cagctgaccg agaggcgcga acttaaggca ttccatgcgg tcgccgacca ttatcgggtc
    10981 cctcaatcgt tgcgtctcgt cgtcgatatc gggtatctcg tccagatcgg cgggtcggca
    11041 ctgactgacg cgaccatccc attcccggaa cccacgtttt ccgaccggag aatgattccc
    11101 agcacgtacg ttccgcaacg aaacgggaat ctgctcttca tcgccgctgc gtgggcggaa
    11161 gtgatcgggg ccgagtctat ctgggcgggg ctggtggagg aggattccag cggttatcca
    11221 gattgccgcc gcagtttctg tgatgcgata gaacgcgcga tagcagaggg caaccacgac
    11281 cagcattccg accccagaat tatcacgccc ctcatcgggt tatccaaagc cgatatcgtg
    11341 cgtaccggtg tacgtctcgc agcaccattc gagaaaacct ggtcgtgtta ccggcgtgag
    11401 gacacggcct gcggggaatg cgacagttgc cggctgagat tgcggggttt tgcgcaggcc
    11461 ggcgtaagag atctgatacg ttacgctgat ccgggcgttt gaaaacgact tcaggacaga
    11521 ttgtattccc gtagtttccg gtacagggta cgttctccta tacccagcga acgagcggct
    11581 tcccgccgat ttccttttgt tgcggccagt gcctgccgga tggcgtcgcg ttcaagatcc
    11641 ctcagtgtca gtgttcgggc aggtgttgtc tcaccggaag gagtatcgtc ttcagaaact
    11701 tcgatatcca ccacgtgcgg ttttgaagct ggcgacgaag cgtcctgcgg agggagtgaa
    11761 gcactggacc agctcctgct cgccacaccg ttccccggcg ctgtgaggat ttgtttcaaa
    11821 tctgccatgt cggcccgcag ttccagcaag gtgcggtaga tgatcccgag ttccatcttc
    11881 tcggcgcccg atcctcgcga cgctaccgga aggagccggt tcgcaaacac cttgtcacgc
    11941 accatgtcag gcaggttttc gggccgtatg atgggcacat cagagaggac gagcacgcgt
    12001 tccacgacag tgcggagttc acggacattg ccgggccagt cgtacgagag gaggtattcc
    12061 atggcttctt tgctgatcac cggaacagcc cggttattct ccgagcagaa ccgttctacg
    12121 aagtgctcaa caagaagcgg aatatcctct ttgcgctccc gcaggggagg cagatgaatc
    12181 tcaaacacgt ggaggcgcca gtacaggtcc gcccggaacg tacccgcgtt gacgagttct
    12241 gccaggtcgc gattggtagc agttattacg cgagcctccg cgcgcaacgg ttcgattccc
    12301 ccgacccttt tgaactcgcg ttcttccaag acgcggagca gtctcacctg aagcgccggg
    12361 cttatgtcac cgatctcgtc aaggaaaacc gtacctttgc cggcagcttc gaaaataccc
    12421 ttcttttcac gaacagcgtc ggtgaaggat cccttttcgt gtccaaacaa ctcgctttct
    12481 acggtcccct cgttcaacgc gctgatgttg agcgccacga agggctcccg acggcgcgga
    12541 ctcaactcat ggattgcgcg cgcaacaatc tccttccccg ttccggtctc tcccgtgatg
    12601 agtacggtcg tagaggtcgg tgcaatctgc gcgatgctta tgagaacctg ctgaatcgcc
    12661 ggtgattgcc ctatcagacc aacccgccgg gcaaattcct gtcggtcccc cggcctcctg
    12721 acagaccgcg ggtacccttc tggcaaaccc tgctggaacg atgtgtccat atacgttatc
    12781 cggttcccga attcagagtg agtagtcagc tcgcagatct gcttcacgcg tgatcagtat
    12841 gtctttgccg cgcatggcaa cataaccgcg ctccttgagc cgtttcatga cgcgcgaaac
    12901 cgtttcccga gatgtgccgg ccatattcgc aatcgcctgt tgattcggcg ggttgtggat
    12961 acagatcccc cgttgtgaag acacgccgcg ttcctccgcg atctgaagga tcaccgaggc
    13021 cactcttccg ctcacgtcca tcagggcgag gctctctatc tttcgatccg cttttcgcag
    13081 gcgggcagta agcgcggcga caactttgat cgccatgtgc ggagaacgct ccaccaaccg
    13141 caggaaatcc cgccgcctca aaataagcac ggtcgtctct tcagtggcga tcacattcgc
    13201 agaacgcggt tttccgtcga gcaatgacat ctccccgaaa aaatcgccct gcccgagcac
    13261 cgccaggatc acttctttcc cgtcctcatt caggatggaa accttgacct ggcctgagag
    13321 aatcgtgaac agcgcttcac cctcgtcctc tgccaggatg atcaggttct ccggcggata
    13381 aaccctctgc gtcacaagcg ttccaatttg tgccagctcg gactctgaca accccgcgaa
    13441 aaatggcacg ttcctcaggt agctgccagg cgcatccatt gcggtccact cctatctcgt
    13501 gtccgtcggt gacgacccgg attccgatcc agaatcgaga ttctggcccg agatgctcat
    13561 gagctggagg gctgctgcac gtcccagatc actctcgcct tccatcgcga tgattttcct
    13621 gtacagcatt ttggcatctt ccaactgacc cagtttctca tttgcctttg ccgcgcggaa
    13681 taacgcggta accgcccatc gcgatccgga ctggctgtga tatgccagct tgagccattc
    13741 gatcaccgct tcgcggtact gacccgcgtt gaagtgcgct tctcccagac cgaaacgagc
    13801 gtctggctcc tcgggcgttc cggcaacgtt gtccagcgcc tgcctgtacg cggcgactgc
    13861 tgccgggaat tcaccgagac gattcaaatc gtccccgatt ttcacccaga cgcgcaccac
    13921 gtgcggactg tcaggaaact tgctgaggaa aaccctcatc gtgcggacgg cggaatccag
    13981 acgctgaatc tgttcgtacg aaagaatcag gttccacatc gcctgggcgg caaactggct
    14041 cgtagtatcc tgctcgagca tccggttgta cgcgtcagcg gcctgg
//
LOCUS       DAOTNG010000039        14070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4243_length_14070_cov_5.132430, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000039 DAOTNG010000000
VERSION     DAOTNG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_14070_cov_5.132430"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="P5179_03140"
     CDS             complement(<1..1326)
                     /locus_tag="P5179_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS94247.1"
                     /translation="MQGIMYLDALASFGKMGPKDEEVSWKLETLLDEMAWCGIHGALV
                     YHNVAREYDPAYGNRMLMEAISGNPRLFPCWVVLPHDCGEMPPPKRLVKEMLAAGVRA
                     VRMAPRSQNYLLEEYVCGELLDALAGEQILLSIGIDQTSYGDLFQIASRRPGLPILMT
                     GCSWASTRNILPLMDKCPNVHIEFSTFQANYALEEFTERYGAERLLFGTDALLKSMGA
                     AKSFVDYAELGEADRKLIAGGNLARLLRLERLPEEYPARTDGPILSRVKEGLPLEHIT
                     VIDSHAHMSHNDARGVGYMPMPRSDAKHMIRRNRRLGIDKVAISSWMGIFADCEEGNV
                     ILKDAMDQFPDDIIGYATLDPNYLTAEEFDRWMRLAHEEWGFKGMKPYNPRVRIPYNS
                     PRYDQWYAYGNEHRLFCLLHSSGGNFINEVADISKRYPEISFLLAHSGGD"
     gene            1610..1686
                     /locus_tag="P5179_03145"
     tRNA            1610..1686
                     /locus_tag="P5179_03145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1644..1646,aa:Arg,seq:cct)
     gene            complement(1702..2631)
                     /locus_tag="P5179_03150"
     CDS             complement(1702..2631)
                     /locus_tag="P5179_03150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS94248.1"
                     /translation="MRHPGLYIRPLEPRDIVPVARLLDESLPRDRVTPRWLKEKAFAD
                     PDYDSALTLCAIDGGNLVGFAHGVARPSHAPVRGWFKWFAVAETHRRTGIATKLFDQI
                     ERVMARRGVRSVSIADSVPNYTMPGVDPRYAEARVFLAQRGYVEGEQRVNMACPLTTA
                     DWRTDEEEDRLAAQGVRVIRAARTDLSHALEFVRTQFPSWTPEVAHCFLREPISLHLA
                     VEGARVIGFAAYDANNPGMAWFGPMGTHPEKRTRGVGGTLLKRCLRDLAAQGHAEAII
                     PWVGPVVFYEKACGAEISRTFVAVSKQLVEERG"
     gene            complement(2720..3445)
                     /locus_tag="P5179_03155"
     CDS             complement(2720..3445)
                     /locus_tag="P5179_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94249.1"
                     /translation="MRKALIFLAIAIAGALAVAGTQSVRRLFSVARTFREVTFERAHA
                     DTLSLPGLAASVVGQKVLADLELDSVQQEHIARLYRRLRESTPADSAAPVGYSPARRR
                     DPAPAAADALRDSIRAVLTREQWKKLQDSRSTVAILRKNWTDYLSDPMVRDVMGQLDL
                     TASQKAQMKSLAKVYTGDLATLLTISDPVVRRMRVEALFDDGWSQARRILTPDQQATV
                     ENYLATHREQIEAGLRGLWLQGL"
     gene            3583..4521
                     /locus_tag="P5179_03160"
     CDS             3583..4521
                     /locus_tag="P5179_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRS94250.1"
                     /translation="MPVSKSSPLATSDRMREEAASITRVTNIGIVMNVIMTLAKILAG
                     WAFGSIALVADGVHSLSDLATDALVIVGTRVGLRPADKRHPYGHGKIETISALLIAFA
                     LVFVGARISFVAGREMYLRHHSYPGIWVLVIAAISVVGKEWLFRITRRVALACNSAAV
                     FANAWHHRSDALGALAVVFGSVAGMMGWGHGDQTAGVVVGIMVVGVGLKIGVDALVEL
                     FDVSADVETVAALEQAISGEEGVRAFHRLRSRLVGREIFVDVHILVDPALSVVQGHRV
                     ADRVEEAIRSSMKRPANIVVHIEPDLPELRTVKPNK"
     gene            4702..5460
                     /locus_tag="P5179_03165"
     CDS             4702..5460
                     /locus_tag="P5179_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRS94251.1"
                     /translation="MAKIPIAIQMYTLREDCSKDFVGTLKAVAKIGYAGVELAGMYGV
                     APKELGKILDDLGLKRAGGHWGVEPLEKEFDKTVEDVKALGSSFVTIPGLPGTYTEDA
                     EAWKRTAVLLSGIGQKLLKEGIQLSYHNHNHEFKLFGGKHGLDILYENSDPKALHAEI
                     DTYWVQYAGVDPATYMKKVANRLSLVHIKDMADDDMKSFAEIGNGILDWDAIFAVAKE
                     AGSKWLIVEQDSCKRPPLESAKISFENLKTMGAV"
     gene            5533..5608
                     /locus_tag="P5179_03170"
     tRNA            5533..5608
                     /locus_tag="P5179_03170"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5566..5568,aa:Ala,seq:ggc)
     gene            5861..6583
                     /locus_tag="P5179_03175"
     CDS             5861..6583
                     /locus_tag="P5179_03175"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRS94252.1"
                     /translation="MSLIIIIGRGHGGTRAMSQTLVDSGVYMGEPLNDSYDLVPPEAM
                     YDACRVMARYVKWHGGFRWDWSQLHSMDIPEKFTGLIQTYLKSVLESPAARKGWKIPE
                     TTLVFPWIVRMFPEAKYIFWIRDPRDSILGGHVTDDLSDFNIPYPLTEDERRRRAISW
                     KYQYDLVKATPKPAHWIEVRFEDFVLKQEETLPRLEDFLGMKLAKIPVRPEAVGRWKT
                     AEGPHTFDFFEPALVEYGYEKG"
     gene            complement(6731..7855)
                     /locus_tag="P5179_03180"
     CDS             complement(6731..7855)
                     /locus_tag="P5179_03180"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS94253.1"
                     /translation="MVLRRNGHPLFTLGMYERPRNDDEWAAWSKAGVNLVCCSTREHL
                     DAAHRWGMFGWVPVPMILAPEDNGAQLAERIRSLADHPALAVWEAPDEAIWHAHRQPT
                     NWATKRLWSLPPEDQRVVQERLDAVVHGLARGSTLVRSIDPGRPLWLNESGANQLTLA
                     RCVSSLDIVSVDAYPVPPRIEKPVHHIGLDIDRFRAVAPNKEVWMVEQAFSWNNLYPE
                     RKKVEAPVYPTLEESRFMAWQAILHGATGLLWWGSSYEDRPAQIVDTVMQVVSEISQL
                     NEYLIGGEVFSVRVVADTRAYPATALGIGHICRRAGDGTLLVLANEDPFDQEAIITGV
                     DWLDVSKMRPVCEPSASLTRIDEGWITPMKGYEVRLYVSG"
     gene            8132..8449
                     /locus_tag="P5179_03185"
     CDS             8132..8449
                     /locus_tag="P5179_03185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRS94254.1"
                     /translation="MVSMEQIEEFGRRIGEQFKPERVILFGSYAYGTPTPDSDVDLLV
                     IMPCTKKPAHHAAEIRLALSAGFPLDLVVREPETLEARVALGDPFLSEIKEKGKVLYE
                     SAG"
     gene            8433..8777
                     /locus_tag="P5179_03190"
     CDS             8433..8777
                     /locus_tag="P5179_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRS94255.1"
                     /translation="MNPLVEEWIETGEGDFATARRELRAKKHPNYNAACFHAQQCAEK
                     YLKARLLMAGTRFPKTHDLEALVKLLLPHEPGLLELLPMAVSLTSLAVDVRYPGHPVD
                     KATAAEALASAT"
     gene            complement(9111..9569)
                     /gene="mscL"
                     /locus_tag="P5179_03195"
     CDS             complement(9111..9569)
                     /gene="mscL"
                     /locus_tag="P5179_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRS94256.1"
                     /translation="MFQEFKKFAMRGNVLDMAIGIIIGGAFGTIVKSLVDDVIMPPIG
                     LLLGGVDFSNFFVVLKDGVEKAGPYAALADAKAAGAVTIAYGAFINSVISFLIVAFAV
                     FLLVKSFNAMQRKQEAAPAAEPTTKECPFCFSAIPIKATRCPHCTSEIRT"
     gene            complement(9654..10502)
                     /locus_tag="P5179_03200"
     CDS             complement(9654..10502)
                     /locus_tag="P5179_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS94257.1"
                     /translation="MVIGSDWHIHSHHSCDQAALRMADLIREAAPNGVTDFGITDHIH
                     TPYNRPDIVASRLEFEGVRDNPRFHFGVEASCVSRWEIDEIARGGHENPVYGLRTGGP
                     PGCELAIALTAEDITELGIEYVIGGTHWPMYVPLERHAVIRDYHRQNMFLAMHPLVDI
                     VAHPWWWHGGWEDAQHNLSAEPWFDDFTCIPESMHDEFAAAAIEHGKKVEINLNALLL
                     NPHYPEGFKLQYLVYLAKLKDRGVQLSLGSDCHAPHYTVGFERAARMLESVGFVEADF
                     WVLEPR"
     assembly_gap    10608..10707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10708..11120)
                     /locus_tag="P5179_03205"
     CDS             complement(<10708..11120)
                     /locus_tag="P5179_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94258.1"
                     /translation="MTKTQHYRETLARLIDWEPFLLAESGLPGPRGNLELAAVVADMG
                     SRAQFERFLSVPDERAQVNSPGEFLVFCGVVGLGRLLAEGDHSLLPRIRRYASDFRWR
                     TREAVAMALQRWGRSDMAALLAEMEHWAAGNRLEQR"
     gene            complement(11129..12562)
                     /gene="proS"
                     /locus_tag="P5179_03210"
     CDS             complement(11129..12562)
                     /gene="proS"
                     /locus_tag="P5179_03210"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRS94259.1"
                     /translation="MPGLTNPSSDYSAWYNEVVLQGELADYAPVRGCMIIRPYGYAIW
                     ELMQQALDGMFKETGHQNVYFPLLIPESFLQKEAEHVEGFAPECAVVTHGGGKKLEEP
                     LVIRPTSETIIWNTYRDWIQSYRDLPLLYNQWANVLRWELRTRLFLRTLEFLWQEGHT
                     AHATQEEAVEETHRMLGVYRRFAEEWMALPVIPGIKTESEKFAGAVQTLCIEAMMGDR
                     RALQAGTSHFLGQNFAKAFDVTYQDEAGGRQHVWATSWGVSTRLIGALIMGHGDEKGL
                     RLPPRLAPYQVVVVPIYKTDEEKTAVLESAHRLVDFLKTSARVKLDDRDTVRPGFKFN
                     EWELRGVPLRVEMGPKDLAKGQVVLARRDTGTKEFLPQEGLAEHVRATMDDIHRTLFE
                     QAKAFRDANTFRVDTWEEFRERTAGEGGSGFLLAHWDGAAETEALIKEETKATIRCIP
                     LDNPQEAGKCVRTGKPSTQRVVFAKAY"
     gene            12776..13369
                     /locus_tag="P5179_03215"
     CDS             12776..13369
                     /locus_tag="P5179_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS94260.1"
                     /translation="MAAGDSELVRQTLKGDKRAFGVLIQRHQDAVFALVSRLIRNPGD
                     VQDVAQDAFIRAYKALPTFRGDAQFGTWLYRIVWNTCLDRREQHARVQEREVQIAADE
                     EDSDGIDGFADEETPLPDAAFEADDVRQRLEACLNRLPVHYRAVLTLYYFEQKSYEEI
                     AMVLDQPMNTIKVHLFRAKSHLKKALLAERSSEEWTE"
     gene            13366..13863
                     /locus_tag="P5179_03220"
     CDS             13366..13863
                     /locus_tag="P5179_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94261.1"
                     /translation="MTCTDFRLLAERRAAGFPLKARQERALERHLAECSACAAVARFE
                     NGLAAAVREFPAVAPPADFADMVLAQLPDPVTVPEPSFREYWWNLALGVLAAGAASWA
                     LWEKAPSWIPWLTGERERTEIADILFKTYMSMGEVSLPTMSVVFLALIGLTAWGASAL
                     AVGER"
     gene            complement(13870..>14070)
                     /locus_tag="P5179_03225"
     CDS             complement(13870..>14070)
                     /locus_tag="P5179_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94262.1"
                     /translation="WSNGVASGMPRARYGLAGANVGADRRVRPRWHTIRTRADTQVRP
                     TCKRHHWTNAILLVTTPCADVE"
BASE COUNT         2641 a         4203 c         4403 g         2723 t
ORIGIN      
        1 atcgcccccc gaatgcgcaa gcaggaagct gatctccgga taacgtttgc tgatatcggc
       61 cacctcgttg atgaaattgc cgcccgacga atggagcaga cagaacaacc ggtgctcgtt
      121 gccgtaggca taccactggt cgtaccgcgg agagttgtac ggaatccgaa cccgcgggtt
      181 gtacggcttc atgcctttga aaccccactc ctcgtgggcc agacgcatcc agcggtcgaa
      241 ctcctccgca gtgagataat tcgggtcgag cgtcgcatac ccgatgatgt cgtccgggaa
      301 ctgatccatg gcgtccttga ggatgacgtt cccctcttcg cagtcggcga atatgcccat
      361 ccaggagctt atggcgactt tgtcaatgcc gaggcgccgg ttgcggcgga tcatgtgctt
      421 cgcatcgctt cgcggcatcg gcatgtaccc cacgccgcga gcatcgttgt gcgacatgtg
      481 cgcgtgcgag tcgatcaccg tgatgtgctc cagcggcagc ccttccttca cgcggctcag
      541 gatgggcccg tccgttcgcg ccgggtactc ctcaggcagc cgttccagcc gcaacagccg
      601 ggcgaggttg ccccccgcga taagcttccg atccgcctcg ccaagttccg cgtagtccac
      661 gaacgacttg gcggcgccca tgcttttgag cagcgcgtcc gtgccgaaaa gcagccgttc
      721 ggcgccgtat cgctcggtga actcctccag cgcgtagttc gcctgaaacg tggagaactc
      781 gatgtgcacg ttggggcatt tgtccatgag cggcaggata ttccgcgtgc tggcccacga
      841 acagcccgtc atgaggatcg gcagcccggg ccgccgcgat gcgatttgaa acaggtcacc
      901 gtacgacgtc tgatcaatgc cgatggaaag gaggatctgc tctcccgcca gtgcatcgag
      961 cagttcaccg cagacgtact cctccagcag gtagttctgc gagcgcggcg ccatacggac
     1021 ggccctcacc cccgcggcca gcatctcctt cacaagccgt ttgggcggcg gcatttcccc
     1081 gcaatcatgc ggcaacacaa cccagcaggg gaacaaccgc gggttgccgg aaatcgcctc
     1141 catgagcatc cggttgccgt acgccgggtc atactcccgc gccacgttgt gatacacaag
     1201 tgccccgtga atgccgcacc acgccatttc gtcgaggagt gtctccagtt tccacgacac
     1261 ttcctcgtcc ttgggaccca ttttgccgaa agacgccagc gcgtccagat acatgattcc
     1321 ctgcatggct atgccttccc ttcaaaaagc cgcggacagg cggatggatt gcacttcatg
     1381 agaatacggc gcagcgggcc gcggagacaa cgcaaagggc cgccgccatt ccggcacatg
     1441 agagatgagg cgacgcgtcg tcttcgtgtc aagggttttc gcgaaaatgg ttcaatcggg
     1501 gtacggtttt ttctggggtg ggtagaaaat gtggcgcggc ggactacgcg gcgttccgat
     1561 cttgccatac cgcggcgccg gccgtacttt tttcatagac cacttgggtg ccccggtagc
     1621 tcagttggat agagcacagg tttcctaaac ctggtgtcgc tggttcaatt ccagtccggg
     1681 gtaccagctt acctctcgcc gttatcctcg ctcttccacc agttgttttg aaacagccac
     1741 gaacgtccgg ctgatttccg cgccacaggc cttctcgtag aacaccaccg gtcccaccca
     1801 ggggatgatg gcttccgcat gcccctgcgc cgccagatca cgcaggcagc gtttcagcaa
     1861 cgtgccgccg acaccgcgcg tgcgcttttc cggatgggtg cccatgggac cgaaccatgc
     1921 catgccgggg ttgtttgcgt cgtacgcggc aaacccgatc acgcgtgcgc cctcaacggc
     1981 aaggtggagg ctgattggtt cacgtagaaa gcagtgtgcg acctcgggcg tccatgaggg
     2041 gaactgggtg cggacgaatt cgagcgcgtg ggagagatcc gttcgggcgg cgcggatgac
     2101 gcgcactccc tgtgcggcca gccggtcctc ttcctcatcc gtccgccagt ctgccgtggt
     2161 taacggacag gccatgttca cgcgctgttc accttcgacg tacccccgtt gcgcgaggaa
     2221 gacgcgggcc tccgcgtacc gtggatccac acccggcatg gtgtaattgg gcactgaatc
     2281 cgcgatactg accgaccgaa ctccccggcg cgccatcacc cgctcgatct ggtcaaagag
     2341 tttcgtcgcg atgcctgttc ggcggtgggt ctccgccacg gcgaaccatt tgaaccatcc
     2401 ccgcaccggt gcatgggatg gacgcgccac gccatgcgcg aaccccacca gattaccccc
     2461 gtcaatggcg cagagggtga gcgcgctgtc gtaatccggg tcggcgaagg ccttttcttt
     2521 caaccagcgg ggagtgacgc ggtcgcgtgg cagcgattcg tccagcagcc gggcaaccgg
     2581 aacgatatcc cgcggttcaa ggggacggat gtacaggcct ggatgtcgca tcggaacggt
     2641 cggctcaaag accctgaacg acaggtggcg cggctctggc tagcacttgt tctggggcca
     2701 ttcaggcgga cgccacttat tacaagcctt gcagccagag gccccgcagg ccggcttcta
     2761 tctgctcgcg atgggtggca aggtagttct cgacggtggc ctgttgatcc ggagtgagaa
     2821 tacggcgcgc ctgtgaccag ccgtcgtcaa acagggcttc cacgcgcatg cggcggacca
     2881 ccgggtcgga gattgtgagc agcgtcgcca gatcgccggt gtacaccttt gccagcgact
     2941 tcatctgcgc cttctgagat gccgtgaggt cgagttgccc catcacgtcg cgaaccatcg
     3001 ggtcgctgag gtagtcggtc cagttcttcc ggagaatggc gacggttgaa cgcgaatcct
     3061 gcagcttttt ccactgttcc cgggtcaata cggcgcggat gctgtcgcgg agcgcatcgg
     3121 cggcagcggg tgccgggtcc ctgcggcggg caggcgagta tccgaccgga gcggcggaat
     3181 ccgcgggggt gctctcgcgc agcctgcggt ataaccgggc gatgtgttcc tgctggacgg
     3241 aatcgagctc aaggtccgcg agcaccttct gtcccaccac cgaagccgcg aggccgggaa
     3301 gggacagggt atccgcgtgg gcgcgttcga atgtgacttc ccggaaggtg cgggcaacgg
     3361 aaaacaggcg tcgcaccgat tgggttccgg caacggccag agcgcctgct attgctatcg
     3421 caagaaaaat caacgctttc cgcattacgc ctcactgccg ccggtcggca gccctgtccg
     3481 ttcgatcacg cggttcaatc acccgcactt ggtgctcgcc ggcgccttct ggatacttaa
     3541 cgatacggac tcaggagaac agttcccaac aaagcagggc ttatgccggt ttcgaaaagc
     3601 tcaccattgg cgacgtcgga ccgtatgcgc gaagaggccg cttcgatcac gcgtgtcacg
     3661 aatatcggca tcgtgatgaa tgtgatcatg actctggcaa agatacttgc cgggtgggcg
     3721 tttggttcca tcgcgctggt tgccgacgga gtgcactcgc tttccgacct tgccacggat
     3781 gccctcgtga tcgtcggaac gcgcgtgggg ttgcggccgg cggataagcg ccacccgtac
     3841 ggccacggca agatcgagac gatcagtgcc ctgctcatcg ccttcgcgct ggtgtttgtc
     3901 ggcgcgcgga tttcgtttgt ggcagggcgg gagatgtacc tccgccatca ctcctacccc
     3961 ggcatctggg tactcgtgat tgcggccatt tcggtggtgg gcaaggaatg gcttttccgg
     4021 atcacgcgca gggtggcgct ggcatgcaac agcgccgcag tgttcgccaa cgcctggcat
     4081 caccgttcgg acgccctcgg cgcgctggcc gtggtgttcg gttccgtggc cgggatgatg
     4141 gggtggggcc acggtgacca gacggccggc gtggtggtgg gaatcatggt ggtcggcgtg
     4201 gggctgaaaa tcggcgtgga cgcgctggtg gagcttttcg acgtaagcgc ggacgtcgaa
     4261 acggttgccg cgctggaaca ggcaatttcg ggcgaggagg gggtccgcgc gtttcaccgc
     4321 ctgcgcagcc gtcttgtcgg ccgggagata ttcgtggacg tacacattct ggttgacccg
     4381 gcattgagcg tggttcaggg gcacagggtt gccgatcggg tggaagaagc cattcgatct
     4441 tccatgaagc ggccggcgaa tatcgtcgtc cacatagagc ccgaccttcc cgagcttcgc
     4501 accgtgaagc cgaacaaata accatccgcc gccgcactgc gccttcgcct gagcttttcg
     4561 gcgtggcgat tttcccgcgc caccacccgc ggaattggtt cggtggcggg caggcgatat
     4621 actttgttgc atgaaaaact gctcgcggag tacggcaaaa aatgccggaa ccgccggccc
     4681 cattggaaaa ggagcctcac cgtggccaaa attcccattg cgattcagat gtacacgctg
     4741 cgcgaggact gcagcaagga cttcgtcggc acgttgaaag ccgtcgccaa aatcgggtac
     4801 gccggtgtgg aactggcagg gatgtacggg gttgcgccga aggaactcgg aaagatactg
     4861 gatgacctcg gcctgaagcg cgcgggcggg cactggggtg tcgagccgct ggagaaggaa
     4921 ttcgacaaga cggttgaaga cgtgaaggcg ctcgggtcgt cgtttgtgac catccccggt
     4981 cttccgggca cctacaccga ggacgccgaa gcgtggaagc gcaccgccgt tctgttgtca
     5041 gggatcggac agaaactgct gaaggaaggc attcagcttt cctaccacaa ccacaaccac
     5101 gagttcaaac tctttggcgg aaagcacggc ctggacattc tgtacgaaaa cagcgatccg
     5161 aaggcactcc acgccgagat cgacacctac tgggtgcagt atgcgggagt tgacccggcc
     5221 acgtacatga agaaggtcgc caaccgcctt tcgctggtgc atatcaagga tatggcggac
     5281 gacgatatga agtcgttcgc ggaaatcggc aacgggatcc tcgactggga cgcgattttc
     5341 gccgtcgcca aagaagccgg atcgaagtgg ttgatcgtgg agcaggattc gtgcaagcgc
     5401 ccgccgctgg agagtgcgaa aatcagcttt gagaacctga aaacgatggg cgcggtgtag
     5461 tcacccggat ggtgttcact tgccacacac cggtttcgtg cctatctttt tagcggcatt
     5521 atttgggaaa caggggctgt agctcagctg ggagagcgcc tgtctggcag acatgaggtc
     5581 acgggttcga tccccgtcag ctccaccata tgacaatacg ggacttgcga gcgttcgtga
     5641 gtcccgtatt ccgttgacat cgtcttcacg ccacggttac ggagttctgc gccgtttcgc
     5701 cccgcgagtt gaccacctga ctgattcgag gtccaccccg cacgttcgac gggtttcccc
     5761 tgcggcacgc gcccgttccg gaaaccaccg tacggtctga caacttcatc ccgccatttc
     5821 ccagaaaatg ggctccagtg gaacaggagg aaagcgcttc atgtccctga tcatcatcat
     5881 cggccgcggc cacgggggca cgcgggccat gtcgcagacg cttgtagaca gcggcgtata
     5941 catgggcgag ccgctgaacg attcctacga cctcgtgccg cccgaagcga tgtacgacgc
     6001 ctgccgcgtg atggctcgat acgtcaaatg gcacggcggg ttcaggtggg attggtcgca
     6061 gctgcactcg atggacatcc cggagaaatt caccgggttg atccagacgt acctgaagtc
     6121 ggttctggaa agcccggcag cacgaaaggg ctggaaaatc cccgaaacga cactggtgtt
     6181 cccgtggatc gtgcggatgt tccccgaagc gaagtacatc ttctggattc gtgacccgcg
     6241 cgacagcatt ctcggcggac acgttaccga cgacctttcc gatttcaaca ttccgtatcc
     6301 cctcacggaa gatgaacgcc gccgccgggc gatttcgtgg aagtaccagt acgatctggt
     6361 gaaggcgaca ccgaaaccgg cgcactggat cgaggtgcgg ttcgaagact tcgtgctgaa
     6421 gcaggaggag acacttccgc ggctggagga tttcctcggg atgaagctgg caaagattcc
     6481 ggtccgcccg gaggcggtgg gccgctggaa aacggctgaa gggccgcaca cgttcgattt
     6541 cttcgagccg gcgctggtgg aatacgggta tgagaagggg taaataacac cctgcgccag
     6601 aacgcgcaga cgccctcacc ccgtcgtgcg gcagtaatgg gtcgtacggg acggatggga
     6661 cacatcggcg gggcggggac ccgacggagc gggtccctcc agaggttgca gcgccccggc
     6721 ggcaggggcg ttacccgctc acgtacaacc gcacctcgta ccccttcatg ggggtgatcc
     6781 agccttcatc aatgcgcgta agtgacgcgg aaggctcgca gaccgggcgc atcttgctca
     6841 catcgagcca gtccaccccg gtgatgatgg cctcctgatc aaacggatcc tcgttggcaa
     6901 gcaccaaaag ggtgccgtcc ccggcgcggc ggcagatgtg tccgatgccg agcgccgtcg
     6961 cggggtaggc gcgagtatcg gccaccacac gcacggagaa cacttccccg ccaatgaggt
     7021 actcattcaa ctggctgatt tccgacacga cctgcatcac cgtgtctacg atctgcgcgg
     7081 gacgatcttc gtacgatgat ccccaccaga ggagcccggt cgcgccgtgc agaattgcct
     7141 gccacgccat gaagcggctc tcttccagcg tcgggtagac gggcgcttcc acctttttgc
     7201 gctccgggta gagattgttc cacgagaagg cctgctccac catccacacc tctttgttcg
     7261 gcgcgaccgc ccggaaccgg tcgatatcga gaccgatgtg gtgaaccggc ttttcgatgc
     7321 gcggcgggac agggtaggcg tccaccgaga cgatgtcgag ggaggacacg caccgggcga
     7381 gggtgagctg gttcgcgccg gattcgttca gccagagcgg acggcccgga tcgattgacc
     7441 gcaccagcgt ggagccccgc gcaagaccgt gcacgaccgc atcgaggcgc tcctgaacca
     7501 ctctctgatc ctcgggtggg agggaccaga ggcgcttggt ggcccagttg gtaggctgcc
     7561 ggtgggcgtg ccagatggct tcgtcgggcg cttcccatac cgcgagggcg gggtggtcgg
     7621 caagggagcg aatgcgttcg gcgagttgcg cgccgttgtc ctccggggcg agtatcatcg
     7681 gcacgggcac ccagccgaac atgccccatc tgtgcgcggc gtcgaggtgt tcccgcgtgc
     7741 tgcagcagac gagattgaca ccggccttgc tccacgcggc ccattcgtcg tcgttgcgcg
     7801 ggcgttcgta catgccgagg gtgaaaagcg ggtgaccgtt gcgtcgcaga accatgggtg
     7861 accctccctg aagtggcggt ttcacggatg tgtgttgatg gtagcacggt gccgttgttg
     7921 ccgcaacgcc tgcacgggag tgacgcggca tggaatgacg gcgaggcagt cgtcaagaca
     7981 acaggatgcc gtggccagcg tgacggtacg gcctgagatg accgtggcgc gcgggaacat
     8041 gccctcggca cgggagagcg cgacgttcgc ccgtgccacc gatgacgtgc tttcgtatac
     8101 tattggtgac acagaaaaag agggggtcgc catggtctcc atggagcaga ttgaggagtt
     8161 cggccgcagg atcggggagc agttcaagcc tgaacgcgtg attctgttcg gttcgtacgc
     8221 gtacggaacg ccgacgccgg attccgacgt ggatttgctt gtgatcatgc cgtgcacgaa
     8281 aaagcctgcg caccatgccg cggagatacg gctcgcattg tctgcggggt ttccgctgga
     8341 tctggtcgtc cgtgaaccag aaacactgga ggcgcgggta gcgctcggtg atccttttct
     8401 ctcggagatc aaggaaaagg ggaaggtgct ttatgaatcc gctggttgag gagtggatag
     8461 agaccgggga aggggatttc gcgacggcac ggcgggaact gagggcaaaa aaacatccca
     8521 actacaatgc agcgtgtttc cacgcccagc aatgcgcgga gaaatacctc aaggcccgat
     8581 tgctcatggc cggaacccgc ttcccgaaaa cacacgatct cgaagctctt gtgaaactgc
     8641 tccttcctca cgaaccggga ctgctggaac tgctgccgat ggcggtttcg ctgacctccc
     8701 tggcggtcga cgtgcgctac cccggccacc cggtggacaa agcaaccgcg gcggaagccc
     8761 tggctagtgc aacgtgatac gcgccgcagt aaggcgatcg ctggggctga atgactgaac
     8821 gaatcctcga tggagccgtg actgcgacaa atcaggcaac tcgcgccccg caaacgagct
     8881 gattcgcctg gcttgtagaa atggcggttc ggttcaagga ggtgttcggg cgacgatagg
     8941 aacgcagcga agacgcagca ggagattctt cacttcattc agaatgacgg cgaggcagcg
     9001 cgttccgatg gttgagaaag agagcggcat caacaaaaca gggggagcgc agggcggtat
     9061 tgtgccgcct gacagcgctc cccttgttgt tccggttgtt gccggagaga tcaggttctg
     9121 atttccgatg tgcagtgcgg gcagcgggtg gccttgatcg gaatggcgga gaagcagaac
     9181 gggcattcct tggttgtcgg ctccgcggcc ggtgcggcct cttgcttgcg ctgcatggca
     9241 ttgaagcttt tcacgagcag gaataccgcg aacgcgacga tgaggaagct gatcacggag
     9301 ttgataaacg ccccgtaggc gatcgtcacg gcgccggcgg ctttcgcatc ggcgagggcg
     9361 gcgtagggac ccgccttttc caccccgtct ttgaggacga cgaagaaatt cgagaaatcg
     9421 acgccgccga ggagaagccc gatcggaggc atgatcacgt catccacgag gcttttgacg
     9481 atagttccga acgcgccacc gatgatgata ccgatggcca tgtcgagcac gttgccgcgc
     9541 atcgcgaact ttttgaactc ctgaaacatt gcgtccctcc ttgtgcagtg ccatccgcac
     9601 ggccgttcct gttgctgcaa ttcagcatgg ccgaccggcc gcgcacccgt gtctcaacgc
     9661 ggttccagaa cccagaaatc cgcttccaca aacccgaccg attcgagcat gcgcgccgcc
     9721 cgctcgaacc cgaccgtgta atgcggcgcg tgacaatccg acccgagtga aagctgcacg
     9781 ccacgatcct tgagctttgc cagatacacg agatactgga gcttgaaccc ttccgggtaa
     9841 tggggattca acagcaacgc gttcaggttg atttcgacct tcttcccgtg ttcgatggcc
     9901 gcagcggcga actcgtcgtg catggattct ggaatacagg taaagtcgtc gaaccagggc
     9961 tccgcggaca aattgtgctg tgcatcttcc cagccgccgt gccaccacca cggatgcgca
    10021 acgatatcaa caagcgggtg catggccagg aacatgttct ggcgatggta gtcgcggatc
    10081 accgcgtgcc gttccagtgg gacatacatc ggccagtgcg tgccgccgat gacgtattca
    10141 atgccgagtt ccgtgatgtc ttccgcagtg agcgcgatgg cgagttcgca ccccggagga
    10201 ccgcctgttc gcaggccgta gacgggattt tcgtgacctc cgcgggcgat ttcatcgatc
    10261 tcccatcggg aaacacaact tgcctccacc ccgaagtgga atcgcgggtt atcgcggacg
    10321 ccttcaaact cgaggcgcga ggctacaatg tccgggcgat tgtacggggt gtggatgtga
    10381 tccgtgatgc cgaaatcggt aacgccgttt ggcgctgctt cgcggatgag gtccgccatc
    10441 ctgagagcgg cctgatcgca cgagtggtgg gagtgaatgt gccagtccga gccgataacc
    10501 atgtgttcct catcacatta cgtcttcagt ctgtccctcc agtaggttac ccaatccgca
    10561 tccatttgtg caagtctgtt cttcttcagg ttttccctca ttatccannn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnncgt tgctcgagcc ggtttcctgc ggcccagtgc
    10741 tccatctccg caagaagagc cgccatgtcg gatctgcccc agcgctggag ggccatggca
    10801 actgcttcgc gagttcgcca gcggaaatcg gacgcgtagc ggcgaatgcg tggaaggagc
    10861 gaatgatcgc cctccgccag caggcgcccc agaccgacaa caccacagaa gacaaggaac
    10921 tcgccgggag aattcacctg cgcgcgttcg tccggaactg aaaggaaacg ctcgaattgg
    10981 gcgcggctac ccatgtccgc cactaccgca gccagctcga gattgccccg cgggcccggg
    11041 aggccggatt ccgcgaggag aaagggctcc cagtcgataa ggcgtgccag cgtttcgcgg
    11101 tagtgctgcg tcttcgtcat ccgttcaatc aatacgcctt ggcgaagacc acccgctgtg
    11161 tcgagggttt gcccgtgcgg acgcatttcc ccgcctcctg cgggttgtcg agcgggatgc
    11221 agcggattgt cgccttcgtc tcttccttga tcagcgcttc ggtttccgcc gcgccgtccc
    11281 agtgcgcaag aaggaacccg ctgcctcctt cgccggcggt gcgttcgcga aattcctccc
    11341 acgtatccac gcggaacgtg ttggcatcgc ggaaggcctt tgcctgctca aacagcgtgc
    11401 gatggatgtc gtccattgtc gcgcggacgt gctctgccag cccttcctga ggcaggaact
    11461 ccttggtgcc ggtatcgcgg cgagccagca ccacctgacc ctttgccagg tctttggggc
    11521 ccatttcgac ccgaagcggc acgccgcgca gctcccactc attgaacttg aacccggggc
    11581 ggacggtatc gcggtcgtcc agtttcacgc gtgcgctcgt tttcaggaaa tccacaaggc
    11641 ggtgcgccga ttcgagaact gccgtcttct cctcgtcggt cttgtagatc ggcaccacga
    11701 ccacctgata cggtgcaaga cgcggaggca ggcgcagccc tttctcatcc ccgtgtccca
    11761 tgatgagcgc gcctatgagc cgcgtgctga cgccccagct tgtcgcccag acgtgctggc
    11821 gaccgcccgc ttcgtcctga tacgtcacgt cgaacgcttt cgcgaagttc tggccgagaa
    11881 agtgtgaggt tccggcctgc aatgcgcggc ggtcgcccat catcgcttcg atgcagagcg
    11941 tttgcaccgc gccggcgaac ttctcggatt cggtcttgat tcccggaatt accggcagcg
    12001 ccatccactc ctcggcaaag cgccggtaca cgcccaacat gcggtgcgtt tcctccacgg
    12061 cttcttcctg cgtggcgtgc gccgtgtggc cttcctgcca gaggaactcg agcgtgcgga
    12121 ggaacaggcg cgtgcgaagt tcccaacgga gaacgttggc ccactggttg tagaggagcg
    12181 gaaggtcgcg atacgactgt atccagtccc ggtacgtgtt ccagatgatt gtttcggagg
    12241 tcggccggat gacgagcggc tcctcaagct tcttgccgcc gccatgggtt accacggcgc
    12301 actccggcgc gaagccttcc acgtgttcgg cttccttctg aaggaagctc tcggggatga
    12361 ggagcgggaa atacacgttc tggtggccgg tttctttgaa catgccgtcg agagcctgct
    12421 gcatcagctc ccagatggcg tatccgtagg ggcggatgat catgcagcca cgcacgggcg
    12481 cataatcagc gagctcgccc tgcagaacga cctcgttgta ccacgcggag taatccgagg
    12541 aggggtttgt caatccgggc atgaagttct tcctgagatg gatggggttt ctgcaagcga
    12601 ccgtgtttgt tatcgaatgg gaatacagaa agatacgcat gaacggggca tctcggccag
    12661 ccggccggcg ccgcgcttcc cgtgccggcc ttgcgccttc ctgcttccac tgtaactttt
    12721 ccacacggaa gaccgtcata tcgttcagtg ggtacgtgat tcaaggggcg cggttttggc
    12781 ggcaggcgac agcgaactgg tgcggcagac actgaaaggc gataaacgcg ccttcggtgt
    12841 gttgatccag cgccaccagg atgcggtgtt cgccctcgtc agccggctca tccgcaaccc
    12901 cggtgacgtg caggacgtgg cgcaggacgc cttcatccgc gcgtacaagg ctctccccac
    12961 gtttcggggc gacgcccagt tcgggacatg gctctaccgc atcgtgtgga acacctgtct
    13021 cgaccggcgc gaacagcacg cacgggtgca ggagcgcgag gtgcagattg ctgcggacga
    13081 agaagattcc gacggtatcg acgggtttgc tgacgaggaa acacccctgc cggatgcggc
    13141 gttcgaagcg gatgacgttc gccaacggct tgaggcgtgc ctgaaccggc tgccggtcca
    13201 ttaccgcgcg gttctgacgc tgtattactt tgagcaaaaa agttacgagg aaattgcgat
    13261 ggttcttgat cagccgatga atacgatcaa ggttcatctg tttcgcgcga aatcgcatct
    13321 caagaaggcg ctccttgccg agcgttcttc cgaggagtgg accgaatgac atgcaccgat
    13381 ttccgcctcc ttgccgaacg gcgtgccgct ggtttccctt tgaaggcccg acaggaaaga
    13441 gccctcgagc ggcatcttgc ggaatgctcc gcctgcgccg ccgttgcgcg gtttgaaaac
    13501 ggccttgcgg ctgcggtgcg ggagttcccg gccgtcgctc cgccggccga tttcgccgat
    13561 atggtgctgg cgcagcttcc cgacccggtg accgtgccgg aaccctcatt ccgggaatac
    13621 tggtggaacc tggcgctggg agtgctggcg gcaggagctg cctcgtgggc gctctgggag
    13681 aaggcgccga gctggattcc ctggcttacc ggcgaaagag agagaacgga gatcgctgac
    13741 atcctgttca aaacctacat gtccatgggg gaggtcagcc tgcccactat gtcggtcgtt
    13801 ttcctggccc tgatcggcct caccgcgtgg ggcgcctccg cccttgcggt gggagagagg
    13861 taggcgctgc tattcgacat cggcgcaagg cgtggtgaca aggaggatag cgttggtcca
    13921 gtgatggcgt ttgcaggtag ggcggacctg tgtgtccgcc cgtgttcgaa ttgtgtgcca
    13981 gcgcgggcgg acacgccggt ccgcccctac gttggccccg gccagcccgt accgcgcacg
    14041 cggcatccct gatgccaccc cgttgctcca
//
LOCUS       DAOTNG010000040        14060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4251_length_14060_cov_4.894752, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000040 DAOTNG010000000
VERSION     DAOTNG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_14060_cov_4.894752"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="P5179_03230"
     CDS             <1..631
                     /locus_tag="P5179_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94263.1"
                     /translation="AGRPEHDMVWAPSLTQAGREYLFILAQTYDPDTTSAAWTFFRNN
                     PLLANASKFPIMTATWPVARDDGSGAPMTWTLGDVWKINPYLVNTPNDVFEYKTTARV
                     AVDSLVDMSKITVVPNPFIIRHELMPNENNPALYFTNLPPVCTVRIYTLSGDLVTVLQ
                     HATSGAAASGTAVWDLRNNNFQRVASGLYLYHVEDASGRTFVGKFAVVR"
     gene            662..1705
                     /locus_tag="P5179_03235"
     CDS             662..1705
                     /locus_tag="P5179_03235"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94264.1"
                     /translation="MRYHRILIAGLASLALCGVASAQSNIGRSGAQFLKISPSARATA
                     LGGAYGSVGRDADAVYHNPAGIAGIRGMNLNLARVNYVADISFNYIAAAMPLGSGALG
                     FQFGSMMMDEMPVTTIQDPTNQLGITFDVGSWVAGITYAQPLTDRLSVGVTGKYVRES
                     IWDMSSSTVALDVGTLYYTGFCNWRFSAVLSNFGRTAKFTGGRLITQYNKYDNPNQSE
                     TVAEDVSMAYELPQAFKLGTAYDFTFTDQMMVTGSLEGAHPIDAPEYLSGGLEYTYAM
                     PQVKLAARAGYRIVQTEAMGSYSSLRLASDSAVLGLGIGVPIAGSNLQFDYTWQNYMK
                     LGNNHLFSDGVSF"
     assembly_gap    1802..1901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1902..2536)
                     /locus_tag="P5179_03240"
     CDS             complement(<1902..2536)
                     /locus_tag="P5179_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS94265.1"
                     /translation="MPKVGILIPAYEAGRTVATVVAACRGIAGISDIVVIDDGSTDDT
                     LQNAKNAGAICIRHPENRGKGAALCTGFAEASKLGWDAAITIDADGQHDPAAIPSFIE
                     EYHRCGAGIIVGTRRRTGGMPVVRRASNWLSSSVVSWLAGQRVPDSQSGYRLIAREVW
                     ETVPLSTNRYDMESEMLVRAARRGFAICSVPIPTVYADETSHFHPFRDTARV"
     gene            2728..3429
                     /locus_tag="P5179_03245"
     CDS             2728..3429
                     /locus_tag="P5179_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRS94266.1"
                     /translation="MFPFFFDPTMLLLLPALAFAIWAQWKVSSTFNRYSRIPSRSRLT
                     GRAVATQILRQNMLNDVEVGHVSGHLSDHYDPTSKTVNLSDSNYASYSVASLAVAAHE
                     VGHALQHAHGYQPVTWRSRLVPVANIGSTAAFPLFFIGLLFGKGLGLFFMDLGIALFL
                     FALLFHIVTLPVEFNASKRALAILEDSGMLAADEIPGAKAVLRAAALTYVASATMALM
                     NLLRLIILRNAMSRD"
     gene            complement(3492..4040)
                     /locus_tag="P5179_03250"
     CDS             complement(3492..4040)
                     /locus_tag="P5179_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94267.1"
                     /translation="MTAIWSLSLALVACAMVAYLLHRRRKDTPARPDSREGFPVGAGG
                     FTPQPLKPRLQVEDWDGYSGLQAALCVKLTDGTFVPIIEAGTVPPASRIQTLSTARAD
                     QEQVPVVLYSVVPGATNHAALVQELLVGPVPKTGEDIRPVDLVCTVDEDGTVWTRAED
                     TQGHDIPCTVMIRGERRIPVRP"
     gene            complement(4037..5188)
                     /locus_tag="P5179_03255"
     CDS             complement(4037..5188)
                     /locus_tag="P5179_03255"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS94268.1"
                     /translation="MRVTYQLPNCRVSADQPVLVGGQEGHFWFPHLFRLRGNRILCEV
                     TVTADEAQGDWPAVLFLSEDKGAHWRRVADLETAGPAATRLGPGTMLLMPYENWPSAP
                     GDRRNTRANGTILRDSAQGGLDVEPAEVTFHGFPRDLADYHKGEVALFTNGNLLPTRD
                     GGLLSTVYATFAGETKYDLLAMVSYDRGFSWQFRSVAAVGREIPDAPEGADESSLLRL
                     ADGRILCVFRVGSRHEFHRVLSEDEGNTWTRPEAVRGAWSVEPRMVRLENGLIALTGG
                     REGLFLFVCADGEGKTWERVNLGAHHNATYPDSAMHFSDVFLEARETPNPPESTAYTG
                     MVAAGPEEVLIAYDRTGNGWDGAPGPRGAVDAVFVVRVRLELAERGERR"
     gene            complement(5216..5824)
                     /locus_tag="P5179_03260"
     CDS             complement(5216..5824)
                     /locus_tag="P5179_03260"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016109307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRS94269.1"
                     /translation="MQAFPRRLYEADTAEAARRLLGCRLVREVDGERMAGIIVETEAY
                     LGTEDLGCHSARGRTNRTEVMFGPPGHAYVYLIYGMYHCLNVVTRPEGDPQAVLIRAI
                     EPVEGLEQMRRNRSRRQRKSSPVDRELANGPGKLCEALGIDGSFNGVDLAGKALRIEL
                     MPPVPNQDIAAGPRIGIAYAGEWAQKPLRFWIRGNPCVSTKG"
     gene            complement(5901..6791)
                     /locus_tag="P5179_03265"
     CDS             complement(5901..6791)
                     /locus_tag="P5179_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRS94270.1"
                     /translation="MRLITRGDLDGLTSAVLITSMEKIDSIELVHPKDMQDGKVEVTG
                     NDIVVNLPFHPKAAMWFDHHFGYDAQEVPAKFKGKYGVSPSAARLVFEYYNHPSLDKF
                     GELVGETDRVDAAQLTLDDILHPKRYILLSYTLDPRTGLGGAFRSYFMHLLDWLKVHP
                     IGEVLKMPEVKEKVDTILKNEKDFQKALKKHSKLDGNIVVTDFRRLAERPVGNRFVVY
                     ALYPEANVWMNLFKGRTADITVISLGHSILNRTCNTNIGQLLSTFGGGGHRGAGTCQV
                     PSKKAGRVIKKIKEILKANG"
     gene            7114..8133
                     /locus_tag="P5179_03270"
     CDS             7114..8133
                     /locus_tag="P5179_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94271.1"
                     /translation="MKTRFVPVCLFASLLALGPATALAQSYQSVANDISDLILIHRYP
                     EAQARLNALQRQYPAATDLQFQELVLLFTWIDDYGIADSLNREFRNQVDAVVTSANRD
                     LARNPNDARALFYKGSALAYRALYRSYVEGIGRNIGSLLTDASSGMAELEKARAADPT
                     FADPLIAIGKYKHWKSKKLPWPFATAQEGRDGIRMLEQAVRMGVLSDGGAEQTLAWIY
                     MSERRYDDAIALVQPYVAKYPTSRFFLEIVARAYQEKGDYVRAGALFGRIMNGLSPDE
                     RARPFVVMKYERWIAKLRLQQGRRDEACEIARRLRSLNYAGVHRDWLDRKMEEVHRIE
                     REACR"
     gene            complement(8233..9282)
                     /locus_tag="P5179_03275"
     CDS             complement(8233..9282)
                     /locus_tag="P5179_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRS94272.1"
                     /translation="MNEGLLLSDLLTHGGLPVASRDDWKVKAEDIRARILNVLGAFPA
                     ETPALEPEVLEEVREDGYVRRLVRYASEPDERVPAYVLLPEGQKGPAPAALCLHQTIA
                     EGKRETVGLLQDTHKAYALHLVRRGYVCIAPDHLAAGERITPGLDAYDTSAFYARHPH
                     WSAVGKAIWDARRAVDFLTTLPEVDPARIGVIGHSLGGHGAVFAAAFDERLKACVSNC
                     GVSTFSGSPNPLAWARDRWYVYFPALRPIFLENRRPPFDMHEFAALIAPRAFLTISSL
                     TDESMPVNPYGLQEFAAQLSRLYQFVGAGGKFAWFYHDRGHSYPPESRALAYEWLDEM
                     LGWHSTEAYDGRPRK"
     gene            complement(9297..>9680)
                     /locus_tag="P5179_03280"
     CDS             complement(9297..>9680)
                     /locus_tag="P5179_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94273.1"
                     /translation="ITFATPGPACLPRSFQRQRIVHQAARFVSRDTPLTMVTGGAYLM
                     PLHLKNGGIHRVAVYNGSPDPARPVVWDSRTRTRPPRVTLLAPLRDPMAGDVTPLHAP
                     SGETGYECTSSLPYHGYAVFEWDDA"
     assembly_gap    9681..9780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9781..11464)
                     /locus_tag="P5179_03285"
     CDS             complement(<9781..11464)
                     /locus_tag="P5179_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94274.1"
                     /translation="MKPRYHLRFQIAPGPNLVADSQILADFCNQHNVEEVVLFVAAEE
                     LNNGLLSETEETQWFDTIRTAKQILENAGIVVSLNPWMTVLHTDRGRHFPPDRTFAPM
                     VSPEGEVSTACASFADPAWRDYIKHLYGRFASLGFRVLWVEDDFRFHNHAPLTWGGGF
                     EQPVIERFAARIGKPVTREEIVKAILAPGKPHPWRAIWMETWREIHLETARIIANAVA
                     DSSPVQSTVGLMSSHPSSHSTEGRDWKALFEAFTVSGRVAHRPHFAPYSEATGKTRDW
                     SFMMLDVQRNFRPPQTEVAPEIENFPFTRWSKSDAQTWSEMALCQFFGSDALLLDLFP
                     FTGNSANREPEIGELLDRSRPALEWIASRFSKDMQTEGIGTVWKEDTHAHVRTTRGTS
                     MSELNADPFVAGNFLLPYGVAASMRRQPVTALMGNAVWALSDDEIAGALAGGTLVDGD
                     ALDILTRRGFAELLGVLFSGWEDRDHSLYSIEEVVSPRAGTDVGLVFNTNVVPRIARV
                     EAMPGAEEWTTIITPARQRVGAGIVAFENRLGGRAITFATPGPACLPRSFQRQ"
     gene            complement(11461..>11982)
                     /locus_tag="P5179_03290"
     CDS             complement(11461..>11982)
                     /locus_tag="P5179_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94275.1"
                     /translation="ISAEGFPTIWYGEDILFSRRIERAGWRLAFSPEAWVYHRRRTTV
                     RAWARQLFRMGRARWWMGRYDRGLLEPLYVVPLLECSALAFAVGVVAITQNIGLLTGS
                     FFGVVAAYALALGADAARRTRHPAAFFAAPLLFALREASYAAGSIAGIFSKIPDLTGA
                     LPRMAAEKRETDT"
     assembly_gap    11983..12082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12083..12668)
                     /locus_tag="P5179_03295"
     CDS             complement(<12083..12668)
                     /locus_tag="P5179_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS94276.1"
                     /translation="MADRPETVRISLVIVTYNRADELIRLLQSLQNDLARPDVELILV
                     DDGSTDGTLERVSHLLAPISDRATVLRQPNSGPGIGRNRGILRASGEIVVFVDTDCVV
                     HPGWLSALIEPFSDPKVGAVGGPDRSQPGDPLLTRLIDYLMTSFLTTGGIRGAKKTRG
                     GSYHPRSFNMAARRDAAISAEGFPTIWYGEDILFS"
     gene            complement(12668..13258)
                     /gene="hisB"
                     /locus_tag="P5179_03300"
     CDS             complement(12668..13258)
                     /gene="hisB"
                     /locus_tag="P5179_03300"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRS94277.1"
                     /translation="MARSAEVHRSTKETNVRIVLSLEGNGVFHGSTSIGFFDHMLTLF
                     ARHGGMSLSLDCKGDTQVDGHHTVEDVGIVLGTAIAQALGEKRGIRRYAAAFVPMDES
                     LARVVVDLSGRPYCVIGAELTPAKIGDFDVELAWEFFRALSVHAKCNLHIDLLRAGNA
                     HHAVEACFKAAARAFSEAWTVVGSESGEIPSTKGIL"
     gene            13427..13906
                     /locus_tag="P5179_03305"
     CDS             13427..13906
                     /locus_tag="P5179_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRS94278.1"
                     /translation="MTRDDVIGFIAQRILRINGHVPWRVHFTSRVVNPERVVRGKNTF
                     PGDSPGCYIQAANGIYIGDNTNIGPGVGLISANHDPTDNTRHLPGPPIRIGRDCWIGM
                     NAVILPGVTLGDGVVVGAGAVVTHDFPDRVVIAGNPARVIREIRDGTNNNEESVPSA"
BASE COUNT         2704 a         4202 c         4070 g         2784 t
ORIGIN      
        1 ggcaggccgt ccagagcatg acatggtgtg ggcgccgagt ctgacgcagg cgggacgcga
       61 atatctgttc atcctggcgc agacttacga tcccgatacc acctctgcag cgtggacgtt
      121 cttccgcaac aatccgcttc tggcgaacgc gtccaagttc ccgattatga cggccacgtg
      181 gccggtcgcc cgtgacgacg gatcgggagc gccgatgacc tggacgctcg gcgacgtgtg
      241 gaaaatcaac ccgtacctcg tcaacacgcc caacgacgtg ttcgaataca aaacgacggc
      301 ccgtgtcgcg gtggattcgc tggtcgacat gagcaagatc accgtcgttc cgaacccatt
      361 catcattcgt catgaactga tgccgaatga gaacaaccct gcgctctact tcacgaactt
      421 gccacctgtg tgcacggtcc gcatctacac gctgtcaggt gatctcgtca cggtgctcca
      481 gcacgccact tccggcgccg ccgcgagcgg aaccgccgtg tgggatctcc ggaacaacaa
      541 cttccagcgt gtcgccagcg gcctctacct gtatcacgta gaggacgcaa gcggacgcac
      601 gtttgtcggc aaattcgcgg ttgtgcgcta ggccaatcgg atgacggaag gagtgacatc
      661 catgagatat catcgcatac tcatcgctgg cctggcctct cttgcgctgt gcggagtcgc
      721 atccgcgcag tcgaacatcg gccgctcggg ggcgcagttc ctaaaaatct ccccgagcgc
      781 ccgcgcaaca gcgctgggcg gcgcgtatgg ctccgtcggc agggatgccg acgccgtgta
      841 ccacaacccg gctggcattg ccggaatccg cggcatgaac ctgaatcttg cccgtgtcaa
      901 ttatgtggcc gacatcagct tcaactacat cgccgcggca atgccgcttg ggagcggtgc
      961 gttgggcttc cagttcggct ccatgatgat ggacgagatg ccggttacca cgatccagga
     1021 tcccacaaac cagctcggca tcacgtttga tgtcggttcg tgggtggccg gtatcaccta
     1081 tgcacagcct ctcaccgacc gcctttccgt cggtgtcacg gggaagtatg tccgggaaag
     1141 tatctgggat atgtcctcgt ccaccgtcgc gttggacgtc ggaaccctgt actacacggg
     1201 cttctgcaac tggcgtttct ccgcggtgct gtccaatttc gggcgcacgg cgaagttcac
     1261 cggaggacgc ctgataacgc agtacaacaa gtatgacaat ccgaaccagt cagaaactgt
     1321 cgctgaggac gtttcgatgg cctacgaact gccgcaggcc ttcaaactcg gcacggcgta
     1381 cgacttcacg ttcaccgacc agatgatggt caccgggtcg ctggaaggcg cacacccgat
     1441 cgatgctccc gagtatctct ccggtggtct ggagtacacc tacgccatgc cacaggtgaa
     1501 gctcgcggca cgcgcggggt atcgcatcgt tcagaccgaa gcgatgggct cgtacagctc
     1561 tctccgcctc gcgtcggatt cagcggtcct cggcctcggc atcggagttc cgattgccgg
     1621 aagcaacctc cagttcgatt acacatggca gaactacatg aagctgggga acaatcatct
     1681 gttctcggat ggtgtgagct tctgagtctg ccgggttttc tgaacaaaag agcggcgggt
     1741 gtcttacgac actcgccgct ttccgtttgt ccctgtgtcc gggggcggga cacgctgccc
     1801 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacgcgcgct gtatcgcgga
     1921 acgggtggaa atggcttgtt tcgtccgcgt agacggtcgg aatgggaacc gagcagatcg
     1981 cgaaaccccg gcgcgctgcc cgcacaagca tttcactctc catatcgtac cggttggtgg
     2041 acaacggaac ggtttcccat acctcgcggg caatcaggcg gtaccccgac tgactgtccg
     2101 gcacgcgttg ccccgccaac caggacacga cggatgagga caaccagttt gaagctctcc
     2161 ggaccaccgg catcccaccg gtccggcggc gcgtgccgac gattatccct gcgccgcagc
     2221 ggtgatattc ttcgatgaac gaaggaatgg cggcgggatc atgctggcca tctgcatcta
     2281 tcgtgatcgc ggcgtcccat cctagcttcg atgcttccgc gaaaccggtg cacaacgccg
     2341 cgcctttgcc acggttttcc gggtggcgga tacagatcgc gccggcgttc ttcgcgtttt
     2401 gcagagtgtc gtctgtggac ccgtcgtcga tgacaacgat atcggagatt ccggcaattc
     2461 cccggcatgc cgccaccacg gtggccacgg tgcgcccggc ttcatacgcg ggtatgagaa
     2521 tacctacctt cggcatgcgt taccctcctc gcgaaccggg aacactccgg aactccgggc
     2581 atggaatttg cgttcaaacg gggcaggctg gacgtaatga gacagtgtgt cggcttgaca
     2641 tgcaacgttg gttcccggta aatacatagt ggtggaagtg aacaccggac aataccagcc
     2701 gcaggctggc taccaggagt gaaatatatg tttccgttct tctttgaccc cacaatgctg
     2761 cttcttcttc ccgcgctcgc attcgccatc tgggcgcagt ggaaggtgag ctcgacgttc
     2821 aatcggtaca gccggatccc atcgcgttcg cggctcactg gacgtgccgt tgccacgcag
     2881 atactccgac agaacatgct gaacgatgtc gaagtcggcc atgtgagcgg acacctgtcc
     2941 gatcactacg atccgacgtc aaaaacagtg aacctctctg acagcaatta tgccagttac
     3001 tccgtggcaa gccttgcggt ggcggcacac gaagttggcc atgcgctgca gcatgcgcac
     3061 gggtatcagc ccgtaacgtg gcgctctcgc ctcgtgcctg tcgccaacat tggctcgacg
     3121 gcggccttcc cgctcttctt catcgggctt ctgttcggca aaggtctcgg attgttcttc
     3181 atggacctcg gtatcgcgct gtttcttttt gcccttctgt tccacatagt tacattgccc
     3241 gtggaattca acgcatcgaa gcgtgcgctg gcgattcttg aagacagcgg aatgctggcg
     3301 gcagacgaaa taccaggggc gaaggctgtt ctgcgggcgg ctgcccttac gtacgtcgcg
     3361 tctgccacaa tggcgctgat gaacctgtta cgccttatca tcctgagaaa tgcgatgtcg
     3421 cgggattgac cctcccgacc gctgcgcgaa gggaatcgtg ggaatgcgtc tgtgttccca
     3481 cgattctctt ttcagggcct gaccgggatt ctgcgttctc cccgaatcat gacggtacag
     3541 gggatatcat gcccctgtgt gtcttccgcg cgtgtccaga ccgttccgtc ctcgtcaacg
     3601 gtgcagacaa ggtccaccgg gcgaatatcc tccccggttt tgggaaccgg cccgacgagc
     3661 agctcttgaa ccagcgcggc gtggttcgtg gcgcccggca ccacggaata gagaacaacg
     3721 ggaacctgct cctggtcagc ccgggcggtg gaaagcgtct ggatgcggct ggcgggaggg
     3781 accgttcctg cttcgatgat gggaacaaac gtcccatcgg tgagcttgac gcacagggcg
     3841 gcctggaggc cgctgtaccc atcccaatcc tccacctgca atcggggttt gagcggctgg
     3901 ggagtgaagc caccggcccc gacgggaaac ccttcgcggg aatccggacg cgcgggagtg
     3961 tcctttctcc gccggtgaag cagatatgcc accatggcac acgcgacaag ggccagtgaa
     4021 aggctccata ttgccgtcat cgtcgctctc cccgctctgc gagttcgaga cggactcgca
     4081 ccacaaacac ggcgtctacc gccccacgag ggcccggtgc tccatcccac ccgttgcccg
     4141 ttcgatcata cgcaatgagc acctcctccg gacctgccgc gaccataccg gtataggcgg
     4201 tgctttccgg tgggttcggc gtttccctcg cttccagaaa cacgtccgaa aaatgcattg
     4261 ccgagtccgg gtatgttgcg ttgtgatggg ctcccagatt gacgcgctcc catgttttcc
     4321 cttcgccgtc ggcacagacg aaaagaaaca acccttccct tccgccggta agcgcgatca
     4381 gaccgttttc cagccttacc atccgtggtt cgaccgacca ggctccgcga acggcctctg
     4441 gtctggtcca cgtgtttcct tcgtcttcgc tcagcacgcg gtgaaactcg tggcggcttc
     4501 caacgcggaa cacgcagagg atacggccgt ccgccagtct cagcaggctt gactcatctg
     4561 cgccttcagg ggcgtccggt atctccctgc caaccgcggc gacggaacgg aactgccagg
     4621 aaaacccccg gtcgtagctt accatggcca gcagatcgta tttcgtttcg ccggcaaatg
     4681 ttgcgtaaac ggtcgaaagc aggcctccgt ccctggttgg caggaggttg ccgttggtaa
     4741 agagcgcgac ctcgcctttg tggtagtccg cgagatcccg ggggaacccg tggaacgtca
     4801 cttctgcggg ctcaacgtcc agtcctccct gtgccgagtc tcgaaggatg gtgccgttcg
     4861 cgcgcgtgtt acgcctgtcg ccgggtgcgg agggccagtt ctcgtacggc atgaggagca
     4921 tggttcccgg tccgagcctg gtcgcggccg gaccggcagt ctccagatcc gcaacacggc
     4981 gccagtgtgc tcctttgtcc tcggagagga agagcaccgc cggccagtcg ccctgtgcct
     5041 cgtcggcagt taccgtcacc tcgcacagaa tgcggtttcc tctcagacgg aacaggtgcg
     5101 ggaaccagaa gtgcccttcc tgtccgccga cgagaaccgg ttggtcagcg gaaaccctgc
     5161 aattgggaag ctgatatgtg accctcacgg ccaatccccc tttcagcccg cgctgtcaac
     5221 ccttcgttga cacgcacgga tttccccgta tccagaagcg caggggtttt tgcgcccatt
     5281 cccccgcgta cgcaataccg atgcgcgggc cagcggcgat gtcctggttg ggaacaggcg
     5341 gcatcagttc gatccggagg gctttgcccg ccagatcaac gccgttgaag ctcccatcga
     5401 ttcccagcgc ctcacagagc ttccccggcc cgttcgcgag ttcgcggtca accggcgagc
     5461 tttttcgctg cctccgactc ctgttcctgc gcatctgctc cagtccctca acgggttcta
     5521 tcgcgcggat gaggactgct tgaggatcgc cttccggtcg tgtcacgaca ttgaggcaat
     5581 gatacatgcc gtatatcagg tagacataag cgtgtccggg cggcccgaac attacctcgg
     5641 tccggttggt tctaccccgc gccgagtgac agccgaggtc ttctgtaccg aggtaagcct
     5701 ccgtttccac aatgataccc gccatgcgct cgccgtcaac ttcgcgaacg agtctgcaac
     5761 cgagcagccg gcgtgccgct tctgcggtgt cagcttcgta cagtctgcgc gggaacgctt
     5821 gtatcatggc ggagacaaag ggaacgcagg gttgtgggag agggcgcctg cgctcgtggc
     5881 aggcgccctc ccgcatgatg tcagccgttt gctttcagta tctctttgat cttcttgatg
     5941 acgcgtccgg cctttttcga ggggacctga cacgtgccgg ctccgcggtg gccgccgccg
     6001 ccgaatgtgc tcaaaagctg accgatattc gtgttgcagg tacggttcag gatgctgtga
     6061 cccagcgaga tcaccgtaat gtcggctgtc ctgcccttga aaaggttcat ccacacgttt
     6121 gcctcggggt acagggcgta caccacgaat ctgttcccca ccggccgctc cgccagacgc
     6181 ctgaagtccg tcaccacaat gttcccatcc agcttcgagt gtttcttcaa ggctttctgg
     6241 aaatccttct cgtttttcag gatcgtatcc accttttcct tgacttccgg cattttgaga
     6301 acttcgccga tcgggtgaac cttcagccag tcaagaaggt gcatgaaata ggaacggaac
     6361 gcgccgccaa ggcccgtgcg agggtcgagc gtgtaggaga ggagaatgta gcgcttcggg
     6421 tggagaatgt cgtccagagt cagttgtgcc gcgtcgaccc ggtccgtctc gcctaccagc
     6481 tctccaaatt tgtcgaggct tggatggttg tagtactcga acacaagccg cgccgcgctg
     6541 ggcgaaacac cgtatttgcc cttgaacttg gccggtactt cctgggcatc atacccgaaa
     6601 tgatggtcaa accacattgc cgctttcgga tggaagggga ggttcacgac gatatcattt
     6661 cccgtaacct cgaccttccc gtcctgcatg tctttcgggt gcaccaattc gatactgtcg
     6721 atcttttcca tcgatgtgat cagaaccgcg ctggtcagac catcaagatc gccacgagtg
     6781 atgagacgca tggcaatatc ctcccttttt gtgtgtgaga tgatccacct gtaatagtgc
     6841 gcgccgattc agatcttttt cgcgcaggcg cgtgcgccaa acgccttaag tatattcttt
     6901 ccttaccttt tgtcaaggag cggccggaac aacgtcgaac ggtgaacggc gcaatctccc
     6961 cctcccttgc ggcgtgtatg tctttcagga ggcgtcttgg cgccggtggg aaggaatgtg
     7021 gtcttggatt tgctgggaac gtactgcaaa cgcgtccagt attttgaaat aagccatcac
     7081 gatatgttca ttggaaaggt ccggtaacgg cacgtgaaaa cgcgattcgt ccctgtctgt
     7141 ctttttgctt cgctcttggc gctcggcccc gccaccgctt tggcgcaatc ataccagtca
     7201 gttgcaaatg atataagcga tctcattctt atccaccgct acccggaagc ccaggcgagg
     7261 ctcaacgcgc ttcaacgcca gtatccggcc gccactgatc tgcagttcca ggaactggtg
     7321 ctgctgttca catggattga tgactacgga atcgcagatt cgctcaatcg tgagttcagg
     7381 aatcaggtcg acgcggttgt cacctcggcg aatcgcgatc ttgcccggaa tccgaacgac
     7441 gcacgtgcgc ttttctacaa gggaagtgct ctcgcgtacc gggcgctcta ccggagctac
     7501 gtggaaggta tcgggcggaa tatcggcagc ctgctgaccg acgcttcatc agggatggca
     7561 gaactcgaaa aagcccgtgc ggccgacccg acgttcgccg atcccctcat tgcgataggg
     7621 aagtacaagc actggaagtc gaagaagctt ccgtggccgt tcgccactgc gcaggagggg
     7681 agggatggga ttcgcatgct cgagcaggcc gttcggatgg gtgtcctttc cgacggtggc
     7741 gccgaacaaa cgctggcctg gatctacatg tcagagcgcc gctacgacga tgcaatcgcg
     7801 cttgtgcaac catatgtcgc gaagtatcct acaagccggt tctttctgga aatcgttgcg
     7861 cgcgcatacc aggagaaggg tgactatgtc cgcgcgggag cgcttttcgg ccggattatg
     7921 aacggccttt ccccggatga acgcgcgcgg ccattcgtcg tgatgaaata tgaacggtgg
     7981 atcgcgaaac ttcgccttca gcagggcaga cgcgacgaag cctgcgaaat cgcccggagg
     8041 ctgagatcgt tgaactatgc cggtgtccac cgggattggc tggacaggaa aatggaagag
     8101 gtgcaccgca tagaacgtga agcgtgcagg tgatgtgggg cgagggtgcc cctgtatcat
     8161 tatcgaaaca ggcgaagcgg cggccgcacc ggagtgtgcc ggcaggtaag gccgagagcg
     8221 caggtcacaa catcatttcc gcgggcgtcc gtcgtatgcc tcggtggagt gccaccccag
     8281 catttcgtcg agccattcgt acgccagcgc gcgcgattct ggcggatacg aatgccctct
     8341 atcgtggtag aaccacgcga acttgcctcc cgctccaaca aactggtaca gccgtgaaag
     8401 ctgagcggcg aactcctgaa gcccgtatgg gttcaccggc atgctttcgt cagtcagaga
     8461 actgatggtc aggaaggcac gcggtgcgat aagcgcggcg aattcgtgca tatcgaacgg
     8521 gggacgccgg ttctcaagaa aaatgggtcg cagcgcggga aagtatacgt accagcgatc
     8581 gcgagcccag gcaagaggat tggggcttcc tgagaacgtc gatactccgc agttggatac
     8641 gcacgctttc agacgttcgt cgaacgctgc ggcgaaaacg gcaccatgcc ccccgagtga
     8701 gtgtccgatc acgcctatcc gcgcgggatc tacttctgga agcgtggtca ggaaatccac
     8761 cgcccggcgt gcatcccata ttgccttccc caccgccgac cagtgcggat gccgcgcgta
     8821 gaacgccgag gtgtcgtacg cgtcaagacc cggtgtgatc ctctcgcccg cggcgaggtg
     8881 gtccggcgca atgcacacgt atcccctgcg aacaagatgc agcgcgtatg ctttgtgcgt
     8941 gtcctgcagc aacccgacgg tctcgcgttt cccttcagcg atagtctggt ggaggcacag
     9001 cgccgccggc gccgggccct tctggccttc aggaagaagg acgtacgcgg gaacgcgctc
     9061 gtcaggttcc gaagcgtaac gtaccagcct gcgtacgtac ccgtcctcgc gaacttcctc
     9121 aaggacttcg ggctccagag caggagtttc ggcagggaac gccccgagga cgttcaggat
     9181 acgcgcacgg atgtcctcgg ccttgacctt ccagtcgtca cgcgaggcga ctggaagccc
     9241 tccatgcgtc agaagatcgg aaagcagcaa tccttcattc attcccgctc tctcctttac
     9301 gcgtcatccc attcaaagac cgcgtagccg tggtagggga ggctgctcgt gcactcgtac
     9361 cccgtctctc cggacggcgc gtgaaggggg gtcacgtccc ccgccatggg atcgcgaagt
     9421 ggcgcaagca acgtgacgcg aggtggccgt gtgcgtgtcc ggctatccca gacaacaggg
     9481 cgagcagggt ccggcgaacc gttgtacacc gcgacccggt ggatccctcc gttcttgagg
     9541 tgaaggggca tcaggtatgc cccgcccgta accatcgtaa gtggagtgtc cctcgagaca
     9601 aatctcgccg cctggtgaac gattcgctgc cgctggaagc tcctcgggag gcatgccggc
     9661 ccgggcgtcg cgaacgttat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 gctgccgctg gaagctcctc gggaggcatg ccggcccggg cgtcgcgaac gttatcgcgc
     9841 gcccgccaag cctgttctca aaggccacaa tgccggcccc gacccgctga cgtgcgggcg
     9901 taataatcgt ggtccattct tccgcacccg gcatcgcctc cactctggca atccgcggta
     9961 ccacattcgt gttgaacacc agcccgacat cggtaccggc acggggagaa accacttcct
    10021 caatggaata gaggctgtgg tcacgatctt cccatccaga aaacagcacg cccagcagct
    10081 ccgcgaatcc ccgccgggtg agaatatcca gcgcatcccc atccaccagc gttccgcccg
    10141 caagcgcgcc tgcgatttcg tcgtcggaca gtgcccagac cgcgttgccc atgagggcgg
    10201 tcaccggctg tcttctcatc gaagcggcaa caccgtaggg cagcaggaag ttgccggcaa
    10261 cgaagggatc cgcattcaat tcggacatgg aagtcccgcg agttgtccgg acatgcgcat
    10321 gcgtgtcttc cttccacacg gtgccgatcc cttcggtctg catgtctttg ctgaaacgcg
    10381 aggcgatcca ctccagcgct ggacggcttc ggtcgagcag ctcgccgatt tccggttccc
    10441 tgttcgcgga attgcccgtg aacggaaaca gatcgagcag cagcgcatcc gatccaaaaa
    10501 actggcagag cgccatctcc gaccacgtct gagcgtcgct tttggaccat cgagtgaacg
    10561 ggaaattctc tatctccggg gcgacttcag tctgcggcgg gcggaaattg cgctggacgt
    10621 ccagcatcat gaaggaccag tcacgcgttt ttcctgttgc ctcactgtac ggcgcgaaat
    10681 gaggccggtg cgcgacgcga cccgacaccg tgaatgcttc gaaaagggct ttccaatcgc
    10741 gtccttcggt gctgtgcgaa ctggggtggg agctcatcag gccgaccgtg ctctgaacag
    10801 gggacgaatc cgcgactgcg ttcgcgatga tgcgcgcggt ttcgaggtgg atttcccgcc
    10861 aggtttccat ccagattgcc cgccacggat gcggtttccc cggcgcgaga atcgctttca
    10921 cgatctcctc acgcgtcacg ggtttgccaa tcctcgccgc aaaacgctcg atgacgggtt
    10981 gctcgaaccc tcctccccat gtaaggggcg cgtggttgtg gaaccgaaaa tcatcttcca
    11041 cccagagtac gcgaaatccg aggctggcga agcggccata gaggtgtttg atgtagtccc
    11101 gccaggcagg gtcggcaaag gacgcacagg cagtgctgac ttccccttcg ggagatacca
    11161 tcggcgcgaa ggtccgatcc ggcggaaagt ggcggccccg gtcagtatgc agaaccgtca
    11221 tccagggatt aaggctcacc acaattcctg cgttttcgag aatctgcttc gcggttcgga
    11281 tggtgtcgaa ccactgcgtt tcctctgtct ccgagagcaa cccgttgttc agttcctccg
    11341 cggcgacaaa cagcactacc tcctcaacat tgtgctgatt gcagaaatct gccagaatct
    11401 gtgagtctgc gaccaggttc ggtcccggag cgatctggaa acgaaggtgg tatcgaggct
    11461 tcatgtgtct gtctcccgct tctccgcggc catgcgtgga agcgcgccgg tgaggtcggg
    11521 gattttcgaa aatatccccg cgatagagcc cgctgcgtaa gaggcttccc gaagggcaaa
    11581 tagaagaggg gccgcaaaaa acgccgccgg gtgccgtgta cggcgggccg catctgcgcc
    11641 aagtgccaag gcatacgcag cgacgacgcc aaagaaggac ccggtgagca gcccgatatt
    11701 ctgcgtaatt gcgaccaccc caaccgcaaa agcgagagcg gagcattcga gcagaggaac
    11761 aacatagagc ggctcgagaa ggcctcgatc atagcgtccc atccaccagc gggctcttcc
    11821 catacggaag agctggcggg cccacgcgcg gacagtggtt cggcggcggt gatacaccca
    11881 cgcctccggc gaaaacgcaa gccgccagcc tgcgcgttca atgcgccggc tgaacaggat
    11941 gtcttcgccg taccagatgg tggggaatcc ttcggccgaa atnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnggctgaac aggatgtctt cgccgtacca gatggtgggg
    12121 aatccttcgg ccgaaattgc agcgtcacgg cgggctgcca tgttgaatga gcgtggatga
    12181 taggatccac cgcgcgtctt cttcgcccct cgaatgccgc cggtggtcag aaaggacgtc
    12241 atcaggtagt cgatcaacct tgtgagaagg gggtcacccg gctggctcct gtctggtccg
    12301 ccgacagcgc cgacctttgg gtctgaaaac ggttcgataa gagcggaaag ccacccagga
    12361 tggacaacgc agtcagtatc tacaaagacg acgatctccc ctgatgcccg cagtattccg
    12421 cgatttcgac cgattccggg tcccgaattc ggttgccgca gcacagtagc gcgatcgcta
    12481 atcggagcaa ggaggtggga gacgcgttcc agcgtgccgt cggtagatcc gtcgtccacc
    12541 aggattaact ccacgtccgg acgggcgagg tcgttctgga gtgactgaag gaggcgaatc
    12601 agctcgtccg cacgattgta ggtgacaatg acaagcgata tgcgcaccgt ctccgggcga
    12661 tcggccatca gagaatgccc ttcgtggaag ggatctcgcc ggattcgctt ccaacgacgg
    12721 tccatgcttc cgagaatgcg cgtgccgccg ctttgaagca cgcttccacc gcatgatgcg
    12781 cgttcccggc cctcaggagg tctatgtgga ggttgcactt ggcatggaca gacagggcgc
    12841 ggaagaactc ccacgcaagc tcaacatcga aatccccaat cttcgcgggg gtgagttcgg
    12901 cgccgatcac gcagtacggg cggcctgaaa gatcaaccac cacgcgcgcc agcgactcat
    12961 ccatcgggac gaatgccgcc gcgtagcgtc gtatgcctcg tttttcgccg agtgcctgcg
    13021 cgattgccgt cccgagcaca atccccacat cctcgaccgt atgatgtcca tctacctgcg
    13081 tgtcaccttt gcagtcgagg gaaagactca tgccgccgtg tctcgcgaaa agggttaaca
    13141 tgtgatcgaa gaagccgatg gaggtgcttc cgtggaatac gccgttgccc tccagcgaaa
    13201 gcacaatacg gacattcgtt tccttcgtgg aacggtgtac ctcagcagaa cgtgccatac
    13261 ctgtaacctc atccttacgc attcggttca aattccggcg aaaccgactg ggttttgccg
    13321 ccgtcggcgc gtacaatcat cattacggga atccccctgc ggcgactact ccgaaagtac
    13381 cgaaacgcca cgggcggggg caacaacttt cggctggtgc tcgggtatga cgcgggacga
    13441 tgtgatagga ttcattgcgc agaggattct gcgcatcaac gggcatgtgc cctggcgggt
    13501 gcacttcacg tcgcgcgtgg tgaatccaga acgggttgtg cgtgggaaga acaccttccc
    13561 cggagattct cccggctgct acattcaggc ggcgaacggt atctacattg gtgacaacac
    13621 caatatcggt ccgggagtcg gcctgatcag cgccaaccat gatccgaccg acaacacgcg
    13681 gcacctgccg gggccgccga tcaggatcgg gagagactgc tggatcggga tgaatgcggt
    13741 catattgccc ggggtcactc tgggagacgg tgtggtcgtg ggagcagggg cggtggttac
    13801 ccacgatttc cccgaccgcg tggtaatcgc gggcaatccg gcacgcgtga ttcgcgaaat
    13861 cagggacggc accaacaata acgaggagag cgtcccttca gcataggaga gacgctctca
    13921 gtcagccgtt cgcaattcag aaggggagat cctcgtcctc ggcggacata tcctccggca
    13981 actcaatccc ggtttctttc tgcttccgcg gtggtgcccc ccggcgcggc gcgctcgttg
    14041 ctgaggagag cggctcctcc
//
LOCUS       DAOTNG010000041        13982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4282_length_13982_cov_5.363108, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000041 DAOTNG010000000
VERSION     DAOTNG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4282_length_13982_cov_5.363108"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="P5179_03310"
     CDS             <1..1016
                     /locus_tag="P5179_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS94279.1"
                     /translation="TFTVTATDPFNEGVYYGLGESPGSIDAMTGVFTWTPDYHVEGPI
                     TIEVTASNRDGTSFANVTVNVYSRYGDATENGTTDAADAAAVLQYSVKLPVQINPILA
                     DVTGNEMVTAYDAALILHKLIINPDFRFPVQNYGQIAPKAVSGLPASLVWERVNGGWA
                     LRATNTFGLMAGEFTLSLPGGAPVNVTGGDMVAFRQDGENLFVSVIGSNFADGTLFFI
                     QTTDNAQPGILSASLNEGAIPVVAAAPSAFALEQNSPNPFNPSTTIRFALPEASQVNL
                     VVYDVNGRAIRTLASGSFSAGMHSVVWDARDDRGREVATGTYIYRIVTDKGTFVKRMT
                     LLK"
     gene            1922..2860
                     /locus_tag="P5179_03315"
     CDS             1922..2860
                     /locus_tag="P5179_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS94280.1"
                     /translation="MARLARLVSISFPGVHGEMPPVERARRAVEAVSARIDEAAYDKP
                     DIIVLPETFTALGGDGGKDWFASAEPIPGPTTDALAAKAKQYNTHIVCPILEVRDGKR
                     YNAAALIDRTGSIVGVYHKMHPTNPELEGGIVPGTTAPAWDTDIGRVGCAICFDLNFR
                     DVADSLAAHRAELVCFCSMYRGGLSTQIWAFEYGFWFISATPAENSVIRNPLGQLVVQ
                     SFAYSPTIVAEANLDCVVCHIDCNQEKIPALKKKYGPLAEIHSISPEAVFLLTSRHPT
                     VSARDMVEEFKFETRTEYWKRSHANRAVALMKARES"
     gene            3248..4180
                     /locus_tag="P5179_03320"
     CDS             3248..4180
                     /locus_tag="P5179_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HRS94281.1"
                     /translation="MASSKPLIWAQLVHLGYNMWADREAPEWNIEYTAAQQTMRFDMS
                     LWDDLMKYLTEVGVNTVVIDLGEGLKYESHPELAVDGSWTGAQLRKKLAEMRALGLTP
                     IPKLNFSTCHDTWLGDYSRCVSSKIYYRVCSDIIAEVCELFDKPALFHLGMDEENQPN
                     QQFYAYSVCRQMELWWHDLYFLAEQAEKGGARPWIWSDYVWDNHDVFYAKMPKSILQS
                     NWYYGHKFTTDITAVKAYNELEEHGYDQIPTGSNWSDPQNFEKTVRYCKDVIAPERLK
                     GFFQTVWKPTVERRRWRHFEGASEVGYGKRAFEE"
     gene            complement(4282..5400)
                     /locus_tag="P5179_03325"
     CDS             complement(4282..5400)
                     /locus_tag="P5179_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRS94282.1"
                     /translation="MNQRYDIAAYYWPAYHDEPRWRRFMPKGEGEWQTVRAAAPKFAG
                     HQQPRVPAWGYEDESDPGVMERKIHAAASHGVNTFVFDWYWYENQPFLEEALNRGFLG
                     APNNDRMRFYLMWANHDATTLWDLEQSHEYRVIWPGAADRAAFDAATGRIIERYFGHS
                     SYYLIDGKPVLSIYELGTLITGLGGLEATKDALGSFRERVRAAGFPGVHLQAILWANI
                     PKSPAILPGGGPPADRAETQDNTIRYLGFDSLTNYQWCHYVTPRGEYRAWGEAAVAAW
                     ERWADEFSVPFFPHVSIGWDTNPRFKDLRPDIVTGGTPEAFRGFLIRAMRHVDTRGLI
                     PPLITVNAWNEWSESSYLEPDTVWGMQYLEAVRDALAT"
     gene            complement(5397..6293)
                     /locus_tag="P5179_03330"
     CDS             complement(5397..6293)
                     /locus_tag="P5179_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94283.1"
                     /translation="MEHACFELAPTVFTGTRVKTGFHCHTVNSDGGLSPADTARTYRE
                     CGFGALCITDHRHVTDTEGLSDTEFICLPSIENGGMPDILAVGVKAPVERTLPLAERA
                     KLLAAQGGFTVAAHPTYCAALPQDYLGCEHLHALEIYNAYCDNAYVNGYATELWDMLL
                     GQGKRVLGLAGDDAHLNPEKGYYSHAGLGWIEVWAREFSQPALLAAMKQGAFFSTQGP
                     VFETIEVEHDAIRVTCSPVKQVRWRTFGHAGFVDHAPEAASLTQSELPDWLKTRVFVR
                     IELLDHRGKRAWSNPFFLKAQQ"
     gene            complement(6376..7338)
                     /locus_tag="P5179_03335"
     CDS             complement(6376..7338)
                     /locus_tag="P5179_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRS94284.1"
                     /translation="MAHCTPRVVASLAFAVLVSGCASTTVQVKVLHPAEIDLGGIRRI
                     ALGEISGTGGEELAGTLVTGLFNTGRFEILDRQHLETLMREHQLAAGGLVNPETAAQT
                     GSLVGSAAIVVGRVTEHRFTETLEVGNPKKDDEGNEHRKYTRRGTARVAATLQITDIA
                     TGRVIAVRQPTAVKSATLTSTDAIPGHIDPSPLLAAAREECVADFLRAVAPHEVSESV
                     EFEDDDDLPGTKRGIEFAKGGDLERAVTTFQEVVALHSTKARTHYNLGLALLCLGRYD
                     EAIASLDLAYGMTASRKYARTLARAREWKANAERLRQQSAPGTP"
     gene            complement(7513..8601)
                     /locus_tag="P5179_03340"
     CDS             complement(7513..8601)
                     /locus_tag="P5179_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HRS94285.1"
                     /translation="MLVKPASARCNLACTYCFYHERESDPYARVGSRRMSESVLERFL
                     AEYLPLAGVQPTLGWQGGEPLLAGLRFFERMIELQEKLRDRSQTISHAIQTNGTLIDD
                     DWARFFHDHRMLVGLSIDGPPDLHDTYRLDSAGRGTHARLMEVAETLFRNGAEVNVLC
                     TVNRATARKPEAVFRYLVAQGFQWLQFIPVVERLPNGQPAPFSVTPAQYGEFLKRIFD
                     LWWNDGTPQVSVRLFDEIVSAAMGHPPMMCQLREACGGIYVVVEYNGDVYPCDFFVEE
                     RWRMGNLTETPLAELVNGEVLRRFAEIKPRATSACDSCPYLTFCRRGCPHYRAIGDRF
                     LDRDYLCEGYRRFYAHALPKLAAKLGRR"
     gene            complement(8640..9134)
                     /locus_tag="P5179_03345"
     CDS             complement(8640..9134)
                     /locus_tag="P5179_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94286.1"
                     /translation="MTGEIPPVGTHTHVQKLLLSWGRQTGHRVWVAKGDRGRYSEGRF
                     LADGCIERIPTFMPARVLSILENVDVVWFPPSGAVPVALFEVEHSTAILGGLMRMNDV
                     VTTLVPPLEGWRFFVVAPARRISRFNGELARPTFQASGLARVCRFLSYDHVVEGMRNN
                     LPLR"
     gene            complement(9131..10087)
                     /locus_tag="P5179_03350"
     CDS             complement(9131..10087)
                     /locus_tag="P5179_03350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS94287.1"
                     /translation="MGSDSTATPLSASVVIMTNEREDDLKRSVTALLAQADPSWELIV
                     VDDTPVLSPAMEAFLAENATRFRYVRLTTKNLPRAGNVGITLARGRIVIFVDDDVIPC
                     EGFVARHVAAYTDPSIGGVMGRIRQHNFPEWEPDPSPRDMGFNAFWHKHFNWKTRQPI
                     AMTRGCNMSFRRDVLFEVGGCDEQFVAEGMRWDTDLGYCVRQAGYTIMYEPDAWLEHL
                     YVPRGGARTRGLDLKRTEKYYQNNFYFLCKHRPPFWSAAYYTLAVFLAGYVRKEVIRQ
                     PALLWQIPRMMCRGMRSARRLARDIPATFLPAPVPDAAPVTP"
     gene            complement(10202..11038)
                     /locus_tag="P5179_03355"
     CDS             complement(10202..11038)
                     /locus_tag="P5179_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS94288.1"
                     /translation="MATRSIIKIDQSLCDGCEQCIPNCPEGAIQVIDGKARLVSDLFC
                     DGLGACIGHCPQGAITIEEREAEPYDERRVMANVVQQGTNTIAAHLAHLRDHNEFDLL
                     AEALAYLKENGIPVPPTGGGKVIATMAPAACPGSKTVDLREAPAQVSREAVTGLPTQL
                     RQWPVQITLVPPHAPFLNGCDLLIAADCVPFAYPDFHQELLKGKVLLVGCPKLDDVRP
                     YEEKLTQIFAQNEVKSITYARMEVPCCGGFVPVIRRALAASGKDIPFQDIVIGIRGNR
                     LS"
     gene            complement(11076..12266)
                     /locus_tag="P5179_03360"
     CDS             complement(11076..12266)
                     /locus_tag="P5179_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94289.1"
                     /translation="MTPRERYVRLFHFQPVDYVPDEEFGWWDETLTAWHKQGLPSEIN
                     DNWKGDFFFGFAQTDWAPVNQGLIPGFEHKVISEDENHVIFIDGSGVTQMANKGGRAS
                     IPKYIRFPLETRDDWEDFKRRMDPTDPRRIPSNIDEIVERLKTAENPVGIGMGSLFGW
                     IRNWMGFENLAMLTIDDPEWVEEMVDYLADFIIACAEPVLQKMEKAGVKLDVVSMWED
                     ICFNNGPMVSPTFFRAVVTPRYKRITDRVRKVGCDLTYLDCDGDVTQLVGPWLEGGVN
                     IMFPIEIRGGSDPQQMRDTYGHEVLLKGGVDKMALIEGKEAILADLKRLDPLVRDGGF
                     IPHVDHRVPPDVTYENYLYYLREKRAWLGIPEPPPYEERLEELRKRDEIAARNFTRTD
                     TVKT"
     gene            complement(12307..13977)
                     /locus_tag="P5179_03365"
     CDS             complement(12307..13977)
                     /locus_tag="P5179_03365"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRS94290.1"
                     /translation="MPTDIEIAQRTTLRPITEVAGALGLAQNDLELYGPHKAKISFDA
                     ISRISAHSRPGKLILVTAMTPTPAGEGKTTLAIGLSQALCRLGKKSLVALREPSLGPV
                     FGVKGGATGGGYSQVLPMEDINLHFTGDLHAVTTAHNLLIAMLDNHLHQGNALGVDVR
                     SICMNRTLDLCDRALRKVIVGTGGYANGVVRESGFEITAASEVMAILALSRDMADLKA
                     RLGRITVAATSSGRPVCASDLGAVGAMAATLRDAMKPNLVQTVEGTPALIHAGPFGNV
                     SFGCNSVVATRLGLAMSDYLVTEAGFGSDLGAEKFMNIKCRTAGVMPQAIVIAATIRA
                     LKYQGGADLKKLESKDLAALEKGMPNLYKHIENMQAHGVSPIVGLNRFATDDQEEISL
                     VLESLTAKGIKVALADVWARGGAGGEELAALVLDAVEKPSSPHFLYEDDLPLAEKIRK
                     IAVTIYGADGVTFAGAVKSKLARYEEQGYGKFPVCIAKTQNSLSDQPALKGRPSGFIV
                     TINDVKAATGAGFVIAYAGNILTMFGLPEHPAAERIDVNDSGEISGLF"
BASE COUNT         2761 a         4493 c         4007 g         2721 t
ORIGIN      
        1 tgaccttcac cgtaaccgca acggatccct tcaacgaagg cgtctactac ggccttgggg
       61 aatcacccgg atccatcgac gccatgaccg gcgtgttcac gtggactccg gactaccacg
      121 tcgaaggccc gatcacgatt gaagtgaccg cctcaaaccg cgatggaacc tcattcgcca
      181 acgtgaccgt caacgtctac agccgctacg gcgatgcaac cgaaaacgga accacagacg
      241 cagcggatgc cgccgcggtg cttcagtatt ccgtgaagct gccggtgcag atcaacccga
      301 tactggcgga cgtgacgggc aacgaaatgg tcactgcgta tgacgcggcg ctcattctgc
      361 acaagcttat catcaacccc gatttccgtt tcccggttca gaactacggc cagattgccc
      421 caaaagccgt ttccggtctt cccgcatcgc ttgtgtggga acgcgtgaac ggcgggtggg
      481 ctctccgcgc cacgaacacc ttcggcctca tggcgggtga gttcacgctc tccctgccgg
      541 gtggtgcacc ggtgaacgtc acgggcggcg acatggttgc cttccgccag gatggcgaga
      601 acctgttcgt ctccgtcatc ggcagcaact tcgccgacgg cacgctgttc ttcatccaga
      661 ccaccgacaa cgcccagccg ggcatcctga gcgcgtcgct caacgaaggc gcgattcccg
      721 tggttgcggc agcaccgtcg gcatttgcgc tcgagcaaaa ctcgcccaac ccgttcaacc
      781 ccagcaccac gatccgcttc gcgctgcctg aggcgtcgca ggtgaatctc gtggtgtatg
      841 acgtgaacgg ccgcgccatc cggacgcttg ccagcggttc gttctccgcc ggcatgcact
      901 cggtggtgtg ggatgcgcgc gatgaccgtg gccgcgaagt tgcgaccggc acctacatct
      961 accgcatcgt caccgacaag ggcacattcg tcaagcgcat gacgctgctc aagtaacgac
     1021 cagcagaact gcgtccacac ggggtgagtg gcaacgctca ccccgctctt ttttgcccct
     1081 tcgccggtag ctgcggtggg acaggcattc ctgcctgtcc ttcccacaaa cccttgccgg
     1141 tgtccggacg cccccctctc cgggtggcgt ctgacgaggg catggagggc cccgctccgt
     1201 cggggccatt tttccctgct gccttgctcc tgccccaaat gcctcatggc ccgcgcactg
     1261 gcgtgtgttc agcgccaacg gcgcgtaaca acacaacccg gtctgcaggg ccggccgtcc
     1321 tgcaaatgct tcgagatgtc tgccccacgc tgccgccttg ctcaaccgtc attcccgcat
     1381 gctcggcgga gcacgaagcg cgggcgggaa tccgaaacca cgggtcgcgc ctgtggtggg
     1441 acaggcattc ctgcctgtcc gtgccacaac ccttgccggt gtcgagacgt tccgccctcc
     1501 gggtggcgtc tgccgagggc atggagggcc ccgctccgtc ggggcctttt ttttcccccg
     1561 ctgtcctgct cctgccccaa atgcctcacg gcccacgcac tggcgtgcgt tcaccggcaa
     1621 cggcgcgtaa caacataacc cggttcgcag ggccgggcgt gatggttccg gatcgcgcaa
     1681 accgccctac cacccctcgc acctcccaca accgtcattc ccgcggaggc gggaatccag
     1741 aaccacggtt cgcgcctgtg gtgggacagg cattcctgcc tgtccttccc acaaaccctt
     1801 gcctgcgggg ttttcccact cattgccctc tgcgccggct ccaccttgcg cctgagggac
     1861 ttcccgctgc actcttcttc aggacattca ggcagaattc tcttgcgaag gagccacagg
     1921 catggcacga cttgcgcgat tggtcagcat cagtttcccc ggagttcacg gcgaaatgcc
     1981 gcctgtcgag cgtgcacggc gcgcggttga ggcggtgtct gcccgaatcg atgaagccgc
     2041 atacgacaaa cctgatatca tcgtcctgcc ggaaaccttc accgcccttg gcggggacgg
     2101 cgggaaggac tggtttgcct ccgcggagcc catcccgggg cccacgacgg acgccctggc
     2161 cgcaaaggcg aaacagtaca acacccacat cgtctgcccg atcctcgagg tacgcgacgg
     2221 caaacgctac aacgcggctg cgctcatcga caggacgggt tccatcgtcg gcgtctacca
     2281 caaaatgcac cccacgaacc cggaactgga gggtgggatc gttcccggaa caaccgctcc
     2341 ggcatgggat accgacatcg gtcgcgtcgg gtgcgccatc tgcttcgatc tcaatttccg
     2401 ggacgttgcc gattcccttg cggcgcaccg ggcggaactc gtctgtttct gctccatgta
     2461 ccgcggcggt ctaagcacgc agatatgggc attcgagtac ggattctggt ttatttctgc
     2521 aacgccggcg gaaaacagcg tgattcggaa cccgctgggc cagcttgtcg tgcagtcgtt
     2581 cgcgtattca cccacgatcg tcgcagaggc gaaccttgat tgcgtcgtgt gccacattga
     2641 ctgcaaccag gagaaaatcc ccgcgttgaa gaagaaatac gggccactgg cggaaatcca
     2701 cagcatctcc ccggaggccg tgtttctcct gacttcccgg cacccgacgg tgtccgctcg
     2761 agacatggtt gaggagttca aattcgagac caggacggag tactggaagc gttctcacgc
     2821 caacagagcg gttgccctga tgaaagcgcg ggagtcatga atcgatgaat agagcgttgg
     2881 tggcgcttgc gtgtgccatc ctgtgcggat gcggcggcag gcgccccgca aatacaacgg
     2941 ccgagccctc tcagccccct cccgcggcaa tcgttcgcac ggaaccgcca cgctatcagc
     3001 acatcccgcg cggcacctac cgcaacgata tccccgaagt ggaaccggtt cgccggcccg
     3061 ttcgatccga cacgacccgg cgcgccgcgc cccggccacc ggagccgacg gaatcactgt
     3121 atacacaacc agttcgcaca actaccccgc gcgtcgggcc atcccgcccc gacagcgcgt
     3181 ccgttcgacc gctgagccct gaataagccc gctccggtca ccatctcaca gcgcgaaagg
     3241 caacaccgtg gcatcctcaa aacctcttat ctgggcgcaa cttgtgcatc tcggttacaa
     3301 catgtgggcc gaccgcgaag ctcccgaatg gaacatcgaa tacaccgccg cgcagcagac
     3361 gatgcgattc gacatgtcgt tgtgggacga cctcatgaag tacctcacgg aggtcggcgt
     3421 caataccgtc gtcatcgatc tcggggaagg cctgaaatac gagagccatc ccgagctggc
     3481 ggtggatggc tcgtggacag gtgcgcagct tcggaaaaag ctcgcggaaa tgcgggcgct
     3541 cgggttgaca ccgattccga aattgaactt ctccacgtgc cacgacacct ggctgggcga
     3601 ttactcccgg tgcgtgtcct cgaagatcta ctacagagtg tgcagcgaca tcatcgcgga
     3661 agtgtgcgag ctctttgaca aacccgccct ctttcacctc ggcatggacg aggaaaacca
     3721 gcccaaccag cagttctacg cctattccgt ctgccggcag atggaactct ggtggcacga
     3781 cctttacttc ctcgcggaac aggccgaaaa aggcggtgcg cggccgtgga tctggtcgga
     3841 ttacgtgtgg gataatcacg acgtattcta cgcgaaaatg ccgaaatcca tcctgcagag
     3901 caactggtac tacggccaca agttcacgac cgacatcact gcagtgaagg cgtacaacga
     3961 acttgaggaa cacggatacg atcagatccc caccggcagc aactggagcg acccgcagaa
     4021 ttttgagaag accgtccgtt actgcaagga tgtcatcgcc ccggaacggc tcaaaggctt
     4081 cttccagact gtctggaaac ccaccgtgga gcgtcgtcgc tggcggcatt tcgaaggcgc
     4141 gagcgaagtc gggtacggga aaagggcgtt tgaggagtag gagaagcgta cagggcgggg
     4201 aaaccgcggc aaagggcgtt ggtcatctcg atacacgggg gcgttcacac cggcgaacgc
     4261 ccccgcactc gatgaccgat gttacgtcgc cagtgcatcg cgcacagctt cgaggtactg
     4321 catcccccag acggtgtcgg gttcaagata gctgctttcg ctccactcgt tccacgcgtt
     4381 caccgtgatg agcgggggaa tcagcccgcg cgtatccacg tggcgcattg cgcgaatgag
     4441 gaaaccacgg aaggcctcgg gcgttccgcc ggtaacgata tccggtctca agtctttgaa
     4501 gcgcgggttc gtgtcccagc cgatggaaac atgagggaaa aacggcaccg agaactcatc
     4561 agcccacctt tcccacgcag cgacggcggc ttctccccat gcccggtact caccccgcgg
     4621 ggtgacgtag tggcaccact ggtagttcgt caggctgtcg aaaccaaggt aacggatggt
     4681 attgtcctgc gtctcggcgc ggtcggcagg cgggccgccg cccggcagaa tcgcgggtga
     4741 tttcgggatg ttggcccaca gaattgcctg cagatgcacg ccgggaaaac cggccgcacg
     4801 aacgcgctcc cggaatgacc ccagtgcgtc cttcgttgcc tcaagaccac ccagaccggt
     4861 gatgagcgtg cccagttcgt agatggagag aacgggcttc ccgtcgatca ggtagtacga
     4921 ggaatgcccg aaatagcgct cgattatcct gcctgtggcg gcatcgaacg cggccctgtc
     4981 ggctgccccc ggccagatga cccggtattc gtgcgattgc tcaagatccc acagcgtggt
     5041 cgcatcgtgg ttggcccaca tgaggtagaa gcgcatccgg tcattgttcg gcgcaccgag
     5101 gaatcctctg ttcagagcct cttcgaggaa cggctggttt tcgtaccaat accagtcgaa
     5161 aacaaacgta ttgacgccat gtgaagccgc cgcgtggatc ttccgctcca tcacccctgg
     5221 atcagattcg tcttcgtatc cccacgcggg cacccgcggt tgctgatgcc cggcgaactt
     5281 cggcgccgcg gcgcggacgg tttgccattc gccctccccc tttggcatga agcgccgcca
     5341 gcggggctcg tcgtgataag ccggccagta gtaggccgca atgtcgtagc gctggttcat
     5401 tgttgtgcct tgagaaagaa agggttcgac caggcgcgtt tcccacggtg atcgagcagt
     5461 tcgatgcgca cgaacacccg tgttttgagc cagtcgggaa gctccgattg cgtcagggag
     5521 gcagcctcgg gagcatgatc cacaaacccg gcgtggccga acgtccgcca gcgcacctgt
     5581 ttcaccgggc tgcatgtcac gcggatggca tcgtgttcca cttcgatcgt ctcgaacacc
     5641 ggtccctgtg tggagaaaaa cgcgccctgt ttcatcgcgg caagcagtgc aggttgggag
     5701 aattcacgcg cccatacctc aatccaaccg agcccggcgt gtgaatagta ccccttctcg
     5761 ggattcagat gcgcatcgtc tcccgccagt cccagaaccc gcttgccctg cccgagcagc
     5821 atgtcccaga gttctgtcgc gtagccgttg acgtaggcgt tatcgcaata cgcgttgtaa
     5881 atctccagcg cgtgcaggtg ctcgcagccg aggtagtcct gcggcagcgc tgcgcaatag
     5941 gtgggatggg ccgctacggt gaacccgccc tgcgcggcca gaagcttcgc ccgttccgcc
     6001 aacggcagtg ttctctcgac tggagctttc accccaacgg cgaggatatc cggcatgccg
     6061 ccgttttcga tggacgggag acagatgaac tccgtatctg agagaccttc cgtgtccgtc
     6121 acgtgacggt gatcggtaat gcagagcgcc ccgaagccac actcgcgata tgttcgtgcg
     6181 gtatcagcag gtgaaaggcc tccatcggaa ttcaccgtgt ggcagtgaaa cccggttttg
     6241 acccgcgtgc cggtgaaaac ggtcggggca agttcaaaac aggcgtgttc cacaggacct
     6301 ccagtattca cgttgaaagc aaccggttct cgacgcattg gcggtttgtc cggtctgcag
     6361 tttttcccgc agtcatcacg gagttcccgg tgcgctctgc tgccgaagcc gttccgcgtt
     6421 ggccttccat tcccgggccc gcgcgagcgt tctggcatat ttgcgggacg cagtcatgcc
     6481 gtacgcaaga tcgagggagg caatagcctc gtcatagcgg ccaaggcaaa ggagggcgag
     6541 gccaaggttg taatgcgtcc gtgctttcgt ggaatgcaat gcaaccactt cctggaacgt
     6601 cgtgacggct cgttccagat caccgccctt cgcgaactca atgccgcgtt tcgttccggg
     6661 gaggtcgtca tcgtcttcaa actcaacact ctcgctcact tcgtgcggtg cgacggcacg
     6721 aaggaaatca gccacgcatt cctcgcgcgc ggccgcaagg agtggactcg ggtcaatatg
     6781 cccgggaatc gcatcggtag aggtcaatgt ggccgatttc accgcggtcg gctgtcgaac
     6841 ggcaatcacc cgcccggtcg cgatgtccgt aatctggagt gtggcggcaa ctctcgcggt
     6901 tccacgcctg gtgtacttcc ggtgttcgtt cccttcgtcg tcttttttcg ggttgccaac
     6961 ctcaagcgtt tccgtgaacc ggtgttccgt cacccgcccc acaacgatcg ccgcacttcc
     7021 caccaggcta ccggtttggg cggctgtttc cggattcacc agacctcctg cggcaagctg
     7081 gtgctcacgc atgagggttt ccagatgctg acgatccaga atttcaaatc gcccggtatt
     7141 gaacagaccc gtcaccagtg tccctgccag ttcctcgcca ccggtgcccg aaatctctcc
     7201 cagggcgatg cgccgaatgc ctcccagatc gatttccgca ggatgaagca ccttcacctg
     7261 cacagtcgtt gaggcgcatc cgctcacaag aacggcgaac gcgagcgaag ccacgacgcg
     7321 tggagtgcaa tgtgccatgg ggtcccccgt tcttgccgac ggcaaccgtc gccgcaggtg
     7381 ctcagtccgc agctggtgag ccgtcgccgg aaacgcgcaa ggagttcgaa tcacccggag
     7441 aatcactcac atggaaaggt agtgaggttc gtgcgtggct ggaaggacgg cggcggcaga
     7501 cgagcgctga tcttacctcc gtcccagctt cgccgccagt ttcggcaggg catgggcgta
     7561 gaaccgcctg tacccctcgc acaggtaatc gcggtcaagg aatcggtcgc caatcgcgcg
     7621 gtagtgggga catccgcgcc ggcagaacgt gaggtaggga caggaatcgc acgccgatgt
     7681 ggcccggggt ttgatctcgg cgaatctgcg caacacctcg ccgttcacga gttcggcaag
     7741 aggagtttcc gtaagattgc ccatcctcca gcgctcttcc acgaaaaaat cgcagggata
     7801 cacatcgccg ttgtattcga ccacgacata gattccaccg cacgcctcac gcaactggca
     7861 catcatcgga gggtgtccca ttgccgccga tacgatctcg tcgaaaagcc gtaccgatac
     7921 ctggggcgtg ccgtcgttcc accagaggtc gaatatgcgc ttcaagaact cgccatactg
     7981 cgcgggagtc accgaaaacg gtgccggttg cccattcggc aaccgctcaa caaccgggat
     8041 gaactgcagc cactgaaatc cctgcgcaac gaggtaccga aacaccgcct ccggcttccg
     8101 cgcggttgca cgattcaccg tgcacagcac attcacctcg gcgccgttcc ggaacagcgt
     8161 ctcggcaact tccatcaacc gggcatgtgt tccccgtccg gccgaatcca gacgatatgt
     8221 atcgtgcagg tctggcggac cgtcaatgct caatcccaca agcattcgat gatcgtggaa
     8281 aaatcgcgcc cagtcgtcgt caatcaacgt tccattcgtc tgaatggcgt gggagatcgt
     8341 ctgggacctg tcccgcagtt tttcctgcaa ttcaatcatg cgctcgaaga acctcagccc
     8401 ggcaaggagc ggttcccctc cctgccagcc gagcgtcggt tgcactcccg caaggggaag
     8461 gtattccgcc agaaaacgct ccagaaccga ttccgacatc cgacgggaac cgacgcgcgc
     8521 ataggggtcg ctctcacgtt cgtggtagaa gcagtaggtg cacgcgagat tgcatcgggc
     8581 ggaggccggt tttaccagca ccccaaggtt ctgaatagca ggatggagcg ccatggaggt
     8641 cagcgaagcg gaaggttgtt ccgcattccc tcaacaacgt ggtcatagct gagaaaccgg
     8701 catacccggg ccagcccgct ggcctggaac gtcggacggg caagttctcc gttgaatctc
     8761 gaaattctgc gcgccggcgc aacaacaaaa aagcgccatc cttccagggg aggcacgagt
     8821 gtcgtcacca cgtcattcat gcgcatcaac cccccgagaa tagccgtgga gtgctccacc
     8881 tcgaacaacg caaccggaac cgctcccgac ggcggaaacc agacgacatc cacgttctcg
     8941 aggatgctga gcacgcgggc cggcatgaac gtgggtatgc gttcaataca cccgtcagcc
     9001 agaaaacggc cttcagagta ccgcccccga tcacctttcg ccacccacac acggtgtccg
     9061 gtctgtcgcc cccacgagag gagaagcttc tgcacgtggg tatgcgtacc gacaggtggg
     9121 atttctccag tcatggtgta accggggcgg cgtcaggcac cggcgccgga agaaaggtgg
     9181 ccggaatgtc ccgcgcaagg cgcctggcgg aacgcatacc ccgacacatc atgcgcggaa
     9241 tctgccacag cagggccggt tgccgtatga cttccttcct cacgtacccc gccagaaaca
     9301 cggccagcgt gtagtacgcc gcgctccaga agggcggacg gtgtttgcaa aggaagtaga
     9361 aattgttctg gtaatacttc tccgtccgct tgaggtcgag cccacgtgtc cgcgctccgc
     9421 ctcggggtac atacaggtgc tccagccatg cgtcaggctc gtacatgatc gtgtagcccg
     9481 cctgccgaac gcagtacccc agatcggtgt cccaccgcat cccctccgcc acgaactgct
     9541 cgtcgcatcc gccaacctcg aacagcacgt ctcgccggaa ggacatgttg cagccacgcg
     9601 tcatcgcgat tggctgacgc gtcttccagt tgaagtgttt gtgccagaag gcgttgaagc
     9661 ccatgtccct agggctgggg tctggttccc actcggggaa attgtgctgg cggatgcgtc
     9721 ccatcacgcc gccgatggaa ggatccgtgt aggcggctac gtggcgagcg acaaagcctt
     9781 cacacggaat cacatcgtcg tcgacgaaaa tcacgattct tccgcgggcc agcgtgatgc
     9841 ctacgtttcc ggcacgcgga aggttcttcg tcgtgagccg gacataccgg aacctggtgg
     9901 cgttttccgc gagaaatgct tccatcgcag gcgaaagcac aggcgtgtca tctactacga
     9961 tgagttccca ggacggatcg gcctgcgcga gtagcgccgt taccgagcgt ttgaggtcat
    10021 cttcgcgctc attcgtcata atgacgacgg aagcagacaa cggagtggcg gtggaatcgg
    10081 aacccatggc aaccttacgg taagggcgga tgaaggacac tggaaacaga tcggacagtc
    10141 taaaaatatc acggggcgtg gcatcggcca cgccccgtga gtaccgaacg cgccctcccg
    10201 ctcaactcaa tcggtttccc cgaattccga tcacgatatc ctggaatggg atatccttcc
    10261 cgcttgcggc cagcgcgcgg cggatcaccg gaacaaagcc cccgcagcag ggaacttcca
    10321 tacgggcata cgtaatggat ttgacctcat tctgagcaaa aatctgcgta agcttctcct
    10381 cgtacggacg cacgtcatcc agtttcggac acccgacaag aagaaccttg cccttcaaaa
    10441 gctcctgatg aaaatcgggg tatgcgaacg gaacacaatc tgccgcgatc agaaggtcgc
    10501 acccgttgag gaagggcgca tgcggtggca ccagggttat ctggaccggc cattgccgca
    10561 gttgggtggg caaccccgtt acggcttcgc gggaaacctg ggcgggcgcc tcgcgcaggt
    10621 ccactgtttt tgaccccgga cacgccgcgg gagccatggt ggcgataacc ttcccgcctc
    10681 cggttggcgg cacaggaata ccgttctcct tcagatacgc aagcgcttcg gcaagcaggt
    10741 cgaactcgtt gtggtcgcgc agatgagcga ggtgcgcagc aatcgtgttc gttccctgct
    10801 gcacgacgtt tgccatcacg cgccgctcgt cgtacggctc cgcttcccgt tcctcgatag
    10861 taattgcacc ctgcgggcag tgcccgatac atgccccgag accatcgcag aaaagatcgc
    10921 tgacgagacg cgctttcccg tcaatgacct ggattgcgcc ctcggggcag ttgggtatgc
    10981 actgctcgca gccgtcacac agactctggt cgatcttgat gatacttcgt gttgccatcg
    11041 tgcctccgcg aacaaaccgg cgtggtggtt ttccactacg tctttaccgt gtccgttcgc
    11101 gtgaagttcc gcgcggcaat ttcgtcacgc ttccgcagtt cttccagacg ttcctcgtac
    11161 ggcgggggct cgggtatgcc cagccacgct cgcttctccc gcagatagta gaggtaattc
    11221 tcgtacgtga catcgggcgg cacgcggtga tccacgtgcg ggatgaaccc accgtcgcgg
    11281 accagcggat ccagccgttt caggtcggcc aggatcgcct ctttgccctc gatcagtgcc
    11341 atcttgtcca cgccgccttt gagcagaacc tcgtgcccgt acgtgtcgcg catctgctgg
    11401 ggatcagaac ccccgcgaat ctcaatggga aacatgatgt tcacgccgcc ctcgagccac
    11461 ggacctacga gctgagtcac gtcgccgtcg caatcgaggt acgtaaggtc gcacccgacc
    11521 ttgcgcacgc ggtccgtgat tcttttgtac cgcggcgtca cgactgcccg gaagaacgtc
    11581 gggctcacca tcggaccgtt gttgaagcag atgtcctccc acatggacac cacatcgagt
    11641 ttgacgccgg ccttttccat cttctggagc accggttcgg cgcatgcgat gatgaaatcc
    11701 gcaaggtaat ccaccatctc ctcgacccat tcaggatcgt caatggtcag catcgcaagg
    11761 ttttcaaacc ccatccagtt gcggatccat ccgaacaggc tccccatgcc gatgccgacg
    11821 ggattttctg cggttttgag acgttccacg atttcatcaa tgttgctcgg gatcctccgc
    11881 ggatcggtgg ggtccatgcg gcgcttgaag tcttcccagt catcgcgcgt ctccagcggg
    11941 aaccggatgt acttcggaat ggacgccctg ccgcccttgt tcgccatctg agtcacgccg
    12001 gacccgtcga tgaaaatgac gtgattctca tcttcactga tgactttgtg ctcgaatccg
    12061 ggtatcagcc cctgattcac cggcgcccag tcggtctgcg cgaatccgaa gaagaagtcg
    12121 cctttccaat tgtcgtttat ctcgctcgga agaccctgct tgtgccaggc ggtcagcgtc
    12181 tcatcccacc agccgaattc ctcatccgga acgtagtcaa cgggttggaa gtggaaaagc
    12241 ctgacgtagc gttcacgtgg tgtcatcggt gcacctctgg ttcgaagttg gaatccatct
    12301 cccgtttcag aacaatccgc tgatttctcc gctgtcgttg acatcgatcc tctcggccgc
    12361 cggatgctcg ggcaagccaa acatggtgag gatgttccct gcatacgcaa ttacaaaccc
    12421 ggcgccggtg gccgctttca cgtcgttgat agtcacgatg aacccgctgg ggcgcccctt
    12481 cagcgcaggc tggtcggaaa gagagttctg cgtcttcgcg atgcacacag ggaatttccc
    12541 gtacccctgc tcctcatagc gcgccagctt cgatttcacc gcgcccgcaa acgtgacccc
    12601 gtccgccccg taaatcgtga ccgcgatctt tctgatcttc tccgcgagcg gcaggtcatc
    12661 ctcatacagg aaatgcgggg acgacggttt ctccaccgcg tccagcacca gcgcggccag
    12721 ttcctcgccg cccgcgccgc ctcttgccca gacgtccgcc agcgccactt tgatcccttt
    12781 tgccgtgagc gactcgagaa cgagggatat ctcctcctgg tcgtccgtgg caaacctgtt
    12841 caaccccaca atcggcgaaa caccgtgcgc ctgcatgttc tcaatgtgct tgtacaggtt
    12901 cggcatgcct ttctcaagag cggccagatc cttcgactcc agcttcttga ggtccgcgcc
    12961 accctgatac ttcaatgctc ggatcgtcgc cgcgatgacg atcgcctgcg gcatcacgcc
    13021 cgcggttcgg cacttgatat tcatgaactt ctcggcgccg aggtcgctgc caaaccccgc
    13081 ctcagtcacc agataatcac tcatcgccag tcccaaccgc gtggcaacca cgctgttgca
    13141 cccgaacgac acgttgccga acggtccggc atgaatgagt gcaggcgtgc cttctaccgt
    13201 ctgaacaaga ttcggcttca tcgcatcgcg caatgtcgca gccatcgcgc cgaccgcccc
    13261 gaggtcagat gcgcataccg gacggcccga actcgtcgcc gcgaccgtaa tccttcccag
    13321 gcgtgccttg aggtctgcca tgtcgcgcga aagagcaaga atcgccatca cttcgcttgc
    13381 cgcagtgatc tcaaacccgc tctcgcgcac gacgccattg gcatagccgc cagtacccac
    13441 gatcaccttc cgcagcgcgc ggtcgcacag gtccagcgtc cgattcatgc agatagaccg
    13501 cacatcaaca ccgagtgcgt tcccctggtg caagtggtta tccagcatcg cgatgagcag
    13561 gttgtgcgcc gtcgtaactg cgtggaggtc gccggtgaag tgcaggttga tgtcctccat
    13621 tggcaatacc tgcgaataac cgccgccggt ggcgccccct ttcacaccga aaaccggtcc
    13681 gagagaaggc tcgcgcaacg ccacgagcga cttcttcccg aggcggcaca gcgcctggct
    13741 cagcccgatt gcaagggtgg ttttcccctc gcccgccggg gtgggtgtca tggcggttac
    13801 aagaatgagc ttccccggtc ggctgtgcgc gctgatccgg gaaatggcgt caaagctgat
    13861 ttttgcctta tggggcccgt acaactccag atcattctgg gcaagaccaa gagcaccggc
    13921 aacttcagtg atggggcgca gcgtagtgcg ctgtgcaatc tcgatgtcgg taggcattgg
    13981 tg
//
LOCUS       DAOTNG010000042        13775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4356_length_13775_cov_5.151166, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000042 DAOTNG010000000
VERSION     DAOTNG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4356_length_13775_cov_5.151166"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="P5179_03370"
     CDS             complement(<1..406)
                     /locus_tag="P5179_03370"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRS94291.1"
                     /translation="MGRILPVRLGVNLDHVATVRQARKTTEPSPVQAALLAELGGADQ
                     ITIHLREDRRHIQDDDLELMRRVTQLPINQEMGVTEEMLGIALRIHPNVACLVPEKRV
                     EVTTEGGLDVVGNRERVARAMERLHAAGIAVSL"
     gene            complement(515..1276)
                     /gene="tatC"
                     /locus_tag="P5179_03375"
     CDS             complement(515..1276)
                     /gene="tatC"
                     /locus_tag="P5179_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRS94292.1"
                     /translation="MKDDAPLLSFWDHLEELRWRIVKSILALLIGGIVSLFFSDRILA
                     LLLLPLDKLQGTVRLVNLSPLSMVMVRLYIAIIAGLLVGLPVIVYQAWSFVAPGLYPH
                     ERRAVPWVLISTFLLFVIGASLSYTIMPFLFRVLVEAGYQGVENFWNIREYMGFLLGF
                     MGAFGVVFEMPVAVYILSVVGIATPAGLRRSRKYAIVLIFVVSAVLTPSPDPFSQIAM
                     AVPLVALFEVSIGVSALVHRRKRRRREAAESENAH"
     gene            complement(1289..2119)
                     /locus_tag="P5179_03380"
     CDS             complement(1289..2119)
                     /locus_tag="P5179_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS94293.1"
                     /translation="MKRYALRGVLAVVLVTMVGCAPKRVERPRGPQQPPMVRIPAGEY
                     IIGTDDGPENERPAHKVRVRAFMIDKFEVTNRAYLQFVRETGSPAPPRWLPDSTILGS
                     TDRLPFDEREADLPVTYVSWQDADAYARWRGCRLPTEAEWEIAARCSTAMLYPWGNDS
                     LATDGKHAANTGGESDGFPGLAPAGSFGNGTSCWGAEDLVGNVWEWTADWYMPAAYRE
                     ASPTGFAEVVSDSLFRQRVIRGGSWFDPPGRVKATTRAGFDPSFRSDIIGFRCARDSE
                     "
     gene            complement(2116..3111)
                     /locus_tag="P5179_03385"
     CDS             complement(2116..3111)
                     /locus_tag="P5179_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94294.1"
                     /translation="MNVRWGVIGAGGVARRRSMPAIRQAKNAELVALMVRDRERADAL
                     AKEFSVPRAYTDWRELLADPEVDAVHIATPVYLHAEQVIAAAQTGKHVLCDKPMGMSA
                     LECRRMTDACAANGVHLQVCFLMRFGSVYQRLRREIQDGRFGTVLEARATIFKALPLA
                     DDAWRLIPEQSGGGPLMDLGAHTIDILTFLLGPMESAVAFAANRATNWRVEDTVSLLL
                     RARSGAIAVVEHSFCAKGGDQTLEVNGTEASALVSTPPPGGEKPFLRVAGPQGVVNEP
                     VPFENYYQLQIEHFAECLSGNVAPLATGEDGIHNLAVIEAAYRSVQTNRVEAVQT"
     gene            3309..3686
                     /locus_tag="P5179_03390"
     CDS             3309..3686
                     /locus_tag="P5179_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRS94295.1"
                     /translation="MRELVETSKAPRPIAPYCQAVKANGFVFVSGQGPLDPATGQIVQ
                     GTIEEQTRLTIETIKAILEAAGTSLENVVKVGVFLQDMKDFRAMNRIYGEYFGEIKPA
                     RTTVHAEAPVDIKIEMDCIAVMP"
     gene            complement(3783..4460)
                     /gene="phoU"
                     /locus_tag="P5179_03395"
     CDS             complement(3783..4460)
                     /gene="phoU"
                     /locus_tag="P5179_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRS94296.1"
                     /translation="MSGHLQREIDNLKKKLLALGAFVEEDVQKAVTAISERNASAARE
                     VIESDPDIDKMEIDIEEDCLKVLALHQPVAIDLRFIVAVLKINNDLERIGDLAVNIAE
                     RAAFLATQERVDIPFDFVGMASKAKTMLARSLDALINGDTELAHSVCAADDEVDQINR
                     EMYYQIQDGIRNNPEQMVCLLHLLSVSRHLERIADHATNIAEDVIYMIAGEIVRHRVE
                     EFTQKRS"
     gene            complement(4498..5277)
                     /gene="pstB"
                     /locus_tag="P5179_03400"
     CDS             complement(4498..5277)
                     /gene="pstB"
                     /locus_tag="P5179_03400"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRS94297.1"
                     /translation="MVETGERTSDAKIVVEHLDFHYGSVQALFDVSVEMAPRKVTAFI
                     GPSGCGKSTFLRTLNRMNDIIPGTRVQGRVLIDGHDIYSNGTDVVALRRSVGMVFQKS
                     NPFPKSIFENVVYGPRIHGEKNSARLREIAEQSLQRAGLWAEVKDRLDQNALSLSGGQ
                     QQRLCIARALAVGPEILLMDEPASALDPRSTARIEDLIGELREDYTIVIVTHNMQQAA
                     RVSDFTAFFYEGHLVEFGETQQIFTMPKKKQTEDYITGRFG"
     gene            complement(5271..6122)
                     /gene="pstA"
                     /locus_tag="P5179_03405"
     CDS             complement(5271..6122)
                     /gene="pstA"
                     /locus_tag="P5179_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRS94298.1"
                     /translation="MRQRSNGFLAIWAMRIIFYLLVSSVGIIVLDILWKGLPSISLSF
                     LLDSPRRSGAEGGIFPAIVGTVYLVTGAIAAALPLGIASAVYLSEYAKQGLFTRGVRF
                     AIITLAGVPSVVFGLFGLGLFVIFLGFGASVLAGSLTLACMILPTIIVASEEALQAVP
                     RELREASLALGATRWQTIWRSVLPYSVSGIMTGSILGIGRAAGETAPILLTAAAFFLP
                     NLPESPFDQVMALPYHLYILATQHPEAAKVRGMQYGTALVLLVLVLGINMAAILLRGR
                     FRKKYRW"
     gene            complement(6119..7003)
                     /gene="pstC"
                     /locus_tag="P5179_03410"
     CDS             complement(6119..7003)
                     /gene="pstC"
                     /locus_tag="P5179_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRS94299.1"
                     /translation="MPRPRTKWTERCVEVTLVVAASTSIILVGLIFLFLFREAAPFVQ
                     HPGLSSLMGDRWIPVSFQKEQFGIYPLVTGSILVTAIATVVAVPFGVIGAVYLSEVAR
                     PAETETLKPFIELLAGIPSVVIGFFGLVVLAPFVKAAFGLETGLNAFTGGLLLGLMAI
                     PTIMSVSEDALRSVPASYKQASLALGGNQIQTIWRITVPAALPGIVAAVMLGMGRVIG
                     ETMAVMMVTGNAAQTTFKPFESVRTMTATIAAEMGEVPFGSDHYRALFWVGIILLVAT
                     FLLNIVAQRVLKRYSVRQ"
     gene            complement(7041..7904)
                     /locus_tag="P5179_03415"
     CDS             complement(7041..7904)
                     /locus_tag="P5179_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRS94300.1"
                     /translation="MRNVTTRTLTAISIGLLGMLAACSGKKGNEVTVVTVKGSDTMVH
                     LVSMWAEAFMKNHRDVEISTTGGGSGTGIAALINGTTDICASSRDIKDTERAQAEQKG
                     ITPVETMVARDGIAIAVNPRNPVQELSLDQLRKMYTGVYTRWSQVGGPNEPITALSRE
                     SSSGTYVFFQEHVLNKEDFAQTVRLMPATSAIIQSVVLDRWSIGYVGIGYAHEAGPQV
                     KILGVKATEAGAAVMPTEAAVHSGEYSIARPLFFYTNGVPTGAAKQFVDFCLGPDGQR
                     IVRETGYVPVQ"
     gene            complement(8130..>8911)
                     /locus_tag="P5179_03420"
     CDS             complement(8130..>8911)
                     /locus_tag="P5179_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRS94301.1"
                     /translation="GSRTSLTIKGSDTMVHLVSTWAEAFMRTNPDVEISVTGGGSGTG
                     IAALINGTTDICAASRNIKDSERARAQQNGRSAFETVVARDGIAIVVHPSNSVSTLSH
                     DQLKKIYTAVYTQWNQVGGPNRPILALSRESSSGTYVFFQEHILEQQDYAQSVRLMPA
                     TSAIIQSVSTDRGAIGYVGIGYAHDAGRRVKVLKIKATESGPAVTPSEATVRSGEYSI
                     ARPLFFYTNGAPTGAAKRFVDFCLSSEGQRIVRETGYVPAR"
     assembly_gap    8912..9011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9012..10153)
                     /locus_tag="P5179_03425"
     CDS             complement(<9012..10153)
                     /locus_tag="P5179_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRS94302.1"
                     /translation="MFRGTRSVLSVGAAGVAAVLLAAAIPSPAADGGALAPSWDKGLV
                     LQTADKEFRIRVGGRIHQDWTFFTSQSDSLTRRFGDLQDGTEFRRARIHVDGIAYGTT
                     EWRAEFDFGGGDASFREVYIGLRGVPGLSTLRLGHMQEPFSLEEITSSNYLVFHEYGL
                     AGVFTPAYNAGLLLGGTFSSGRAGWAAGVFRDSDNYGKNSGDGKYAFTGRVVFAPAYS
                     KEQKRVIHLGFSGSTRNPNGGVVKYSRKPEVNLAPNFLNTPTIAAERVNLFGVETLVS
                     QGRFFAQAEGIGSRVTRTGGGSATFTGGYAYAGWFLTNDHRPYKPGQGVADRVVPTRN
                     FDPRKSEWGALAVAARVSYLDLDDSGIKGGTLTDGTLGLDWFLNPNT"
     gene            10413..11474
                     /locus_tag="P5179_03430"
     CDS             10413..11474
                     /locus_tag="P5179_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRS94303.1"
                     /translation="MIPYSLTQPQPFRARRLSSYDRAGGNNDWIPIGGRSTCVIGDIR
                     GPGIISHLYFTVNSPDPHYLRSLVLRCFWDDEPFPSVECPLGDFFCVGHGAVSTFENA
                     AFSMAANKSKTYGRFAPMNCWVPMPFSERARLEITNDSDIPVIAFYFHIDYQEYEQLP
                     GTPWYLHATWRRENPCDGWKGTGSVVWGPEWAARHQTEEDSNLTGSGNYVILETRGRG
                     TYLGCNLSVDNLYKGWWGEGDDMIFVDGEVWPPSLHGTGTEEYFGQGWGMQDVQHQYY
                     GLSYHEREDFNDRGKVTVYRFHVADPIPFRESIRVTLEHGHANDRSDDYASTAYWYQA
                     EPHVPFAALPDVGARLPNP"
     gene            complement(11477..12253)
                     /locus_tag="P5179_03435"
     CDS             complement(11477..12253)
                     /locus_tag="P5179_03435"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRS94304.1"
                     /translation="MSAANTVFQLDGRVAIVTGGGRGLGEVFCRTFADAGATVVVADV
                     VLANAERVASDLVSAGHRAVPVHLDVRSTASVNAMVERTIAEFGQVDVLMNNAGIVIN
                     TSAEEMTDAQWEDVLAVDLTGVFKCCRAVAPHMIARKRGSIINIGSMSGVVSNHPQPQ
                     CSYNAAKAGVIMLSKSLAGEWAQYGIRVNTISPGYMETEMTLRGRQNPRENDVWIENT
                     PMKRCGRPEELGPLALYLASDASSFMTGANILIDGGYTVW"
     gene            complement(12273..13283)
                     /locus_tag="P5179_03440"
     CDS             complement(12273..13283)
                     /locus_tag="P5179_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94305.1"
                     /translation="MTERERFFRELDLDGDGTVNSDVGITYPVWNEYKERLDWMERFS
                     RYVHVGTSRTPSRPAYSKWTDPWGCEWLYPLESLDGLVMRHPVAEWDALTSYKGPNSA
                     EFTDWSEVEKKFKAAKARGEVARGGTDHGFVFLRLTYLRGFNAFMEDVGSRDERLFDL
                     IGVVEDYWFDVAKRYVEAGADLVSFGDDLGLQNRLPISPNAWRTFIKPSYMRIFQYLR
                     SNGVRVHLHTDGYIVDIIPDLIECGVSILNPQELVNGLETLRELAWGRVFLDLDIDRQ
                     SITPFGTPEECERHIGRCVKTLGSPRGGLHLIWGVYPHTPYENIEAVVRAMDSYSEYW
                     KR"
     gene            complement(13301..13642)
                     /locus_tag="P5179_03445"
     CDS             complement(13301..13642)
                     /locus_tag="P5179_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94306.1"
                     /translation="MTMTNVLWLVIFWAMQVVAALSFKQGSLPDASRTLWFIIGNVFG
                     ASSIWFMMELYKTMNVNVAMGLAVGGAFLLSQLATALIFKSNLSFVQYIGLIAITGGM
                     YAVSAGATTHP"
BASE COUNT         2917 a         4194 c         3724 g         2840 t
ORIGIN      
        1 acaatgacac cgcaatacct gccgcatgga gccgttccat ggcgcgcgca acccgttcgc
       61 ggttgccgac gacgtccagc ccaccttcag tcgtgacttc aacgcgcttt tccggtacca
      121 ggcacgcgac attcgggtga attcggagtg cgatacccag catttcctcg gtcacgccca
      181 tttcctgatt gatcggaagc tgcgtgacgc ggcgcatgag ttcgaggtca tcatcctgaa
      241 tatgccgccg gtcctcacgg aggtgtattg ttatctggtc ggctccgccg agttcggcca
      301 gcagagctgc ctgcaccgga ctcggttctg tcgttttccg cgcctgacgg acggtggcga
      361 cgtgatcgag attgactcca agacggacag gaagtattct gcccatttcc ctgctccagt
      421 aacgcggtgc ggtaagggta ttcgtcgtgc gtaagaagta gaaaatacca ctcatgcgcc
      481 gataaaccaa acgcggacaa actgcccgct ccacctagtg agcgttttcc gattctgcag
      541 cttctctgcg acgtcttttg cgccggtgca caagtgcgga tacgccgatg ctgacctcga
      601 agagggccac aagagggact gccatcgcaa tctgactgaa cgggtccggg gaaggcgtca
      661 gcacggcgga gacgacgaaa atgaggacga tggcgtattt ccgcgatcgg cgcagacctg
      721 ccggcgtcgc gatccctacg acggacagaa tgtatacggc caccggcatt tcgaacacga
      781 cgccgaaggc acccatgaat cccaacagaa atcccatgta ttcacgaata ttccagaaat
      841 tctcgacgcc ctgataaccg gcttcgacga ggaccctgaa caaaaaaggc atgatcgtat
      901 aggagaggga tgcgccaatc acaaagagca gaaacgtgct tatcagaacc cagggtaccg
      961 ccctgcgctc gtggggatac agaccgggcg caacgaagga ccatgcctgg tacactatca
     1021 ccggcaggcc cacaagaagc cccgcgatga tggcaatgta caggcgaacc atgaccatgc
     1081 tcagggggga gagattgacg aggcgaacgg tcccctggag tttatcaagc gggaggagca
     1141 ggagcgcgag aatgcgatca gaaaagaaca gcgaaacgat tccgccgatg agaagcgcaa
     1201 ggatggactt gacgatgcgc caccgcaact cctcgaggtg gtcccagaag ctcaggagcg
     1261 gtgcgtcgtc cttcatctga gggagaacct attcagaatc gcgggcacaa cgaaacccga
     1321 taatgtcact ccggaaggat gggtcaaaac cagcgcgcgt cgtcgctttc acacgccccg
     1381 gtggatcgaa ccacgaacct ccccgtatca cccgctgtcg aaagagacta tctgaaacca
     1441 cctccgcgaa tccggttggt gaagcttccc tgtacgctgc cggcatgtac caatccgctg
     1501 tccattccca cacatttccc acgagatcct cggcgcccca gcatgatgtg ccgttcccaa
     1561 aactgcccgc cggagccagc cccgggaatc cgtcgctctc gccgcccgtg tttgccgcgt
     1621 gcttcccgtc cgtggcgaga gaatcattcc cccacggata gagcatcgcc gtggaacaac
     1681 gcgcggcgat ttcccactcc gcttccgtcg gaagtctgca gccccgccag cgcgcgtacg
     1741 catcggcgtc ctgccacgaa acatacgtca ccggcaaatc ggcttcccgc tcgtcgaatg
     1801 gcagacgatc cgtggaaccc agaatcgtgc tgtcgggaag ccagcgtggt ggtgccgggg
     1861 acccggtctc gcgcacaaat tgtaaatacg cgcggttggt cacctcgaac ttgtcgatca
     1921 tgaacgcacg gactcgcact ttgtgtgcgg gacgttcgtt ttccgggccg tcgtcagtgc
     1981 cgattatgta ttcccccgca ggaatccgaa ccataggtgg ctgctgcgga ccgcgcggcc
     2041 gctcgacccg tttcggagca cacccgacca tggtcacgag aaccactgcg agaacaccac
     2101 ggagcgcgta tcgcttcatg tctgcacagc ctccaccctg ttcgtctgaa ccgaacgata
     2161 cgccgcttcg atcaccgcga ggttgtggat accatcctcg cccgttgcaa gcggagcgac
     2221 gtttccggaa aggcattccg cgaagtgctc gatttgcagt tggtaataat tctcaaaagg
     2281 caccggttca ttcaccacac cctggggacc tgcgaccctg aggaatggct tttctcctcc
     2341 cggcggcggc gtcgacacga gcgcgctggc ctccgttcca ttcacctcga gcgtctgatc
     2401 gccgcccttc gcgcaaaaac tgtgctccac cacggcaatc gctcccgacc tcgctcgcag
     2461 caacagcgac accgtgtctt ccacccgcca gtttgtcgct cgattcgccg caaacgcgac
     2521 cgcggactcc atgggcccca gaaggaaggt cagtatgtct atggtgtgtg ctccgaggtc
     2581 catcagtggc ccgccgccgc tctgttcggg tatcaatcgc caggcgtcgt cagcaagcgg
     2641 gagcgctttg aagatcgtgg ctctggcctc aagcactgtc ccgaatcgtc cgtcctggat
     2701 ttcccttcgg aggcgctgat acacagaacc gaacctcatg aggaagcaca cctgcagatg
     2761 gactccgttc gccgcgcagg cgtcggtcat gcgccgacat tcgagcgcgg acatacccat
     2821 cggcttatca cacagaacgt gcttccctgt ctgagcggcc gcaatcacct gttcggcatg
     2881 caggtacacg ggcgtggcga tgtggactgc atccacctcc gggtcggcaa ggagttcgcg
     2941 ccagtccgtg tatgcgcgcg gcacggagaa ctccttcgcc agagcgtccg ctctctccct
     3001 atcccgcacc atcagcgcga caagttcggc attcttcgcc tgtcggatcg ccggcatact
     3061 tcttcgtcgg gcgacgcccc ccgctccgat cactccccac cgaacattca tgcgttcttc
     3121 ctccgctccg ggcgtgattc aaatgcttcg gaccccaaaa gcactttgaa tgtagaactt
     3181 cgtgtgtggc ttcacaaccg gcgcgccgtt gatcccgctt gggcgcccgg ttgagacccg
     3241 gtatattggt ttctacgcac agtcaatcac tcttgcatca gtggagccat cttccgcgga
     3301 ggttcatcat gcgcgaactc gtcgaaacaa gcaaagcgcc gcgacccatc gcaccgtatt
     3361 gccaggccgt gaaggcgaac ggtttcgtgt tcgtctctgg acagggtccg ttggacccgg
     3421 ccaccgggca gatcgttcag ggcaccatcg aagaacagac gcggctcacg atcgaaacca
     3481 tcaaggcaat ccttgaagcg gcgggaacga gtctggaaaa cgtggtgaag gtaggtgttt
     3541 tccttcagga catgaaggat ttccgggcaa tgaatcgcat ttacggggag tatttcggag
     3601 aaatcaagcc agcacgtacg actgttcacg ccgaagcgcc cgttgatatt aaaatcgaaa
     3661 tggattgcat agcagtgatg ccgtgagtag gtgccggctg acattagtga ctcttcttca
     3721 gaaagaaagg ggtggtccca gaaccacccc tctccacgca ggactctgcg gcgtcatctg
     3781 cgtcacgacc ttttctgggt gaactcctcc actctgtgcc ggactatctc ccctgcgatc
     3841 atgtaaatca cgtcctccgc gatattcgtc gcatggtccg cgatgcgttc caggtgccgt
     3901 gagacactca gaagatgaag cagacacacc atttgttcgg ggttattccg aataccgtcc
     3961 tgaatctggt aatacatctc tctgttaatc tgatcgactt cgtcatccgc agcgcacacc
     4021 gaatgcgcga gttccgtgtc gccatttatg agcgcatcca ggctccgcgc gagcatcgtt
     4081 tttgcttttg aggccatgcc tacgaaatcg aacgggatgt ccacccgttc ctgcgtggcg
     4141 agaaatgctg cccgctctgc aatattgact gccagatcac cgatccgctc caaatcgttg
     4201 tttatcttga gaaccgcgac aatgaatcga agatcgattg cgaccggttg gtgtaacgcg
     4261 agcaccttca ggcaatcctc ttcaatatcg atctccattt tgtcgatatc cgggtccgat
     4321 tcgatcactt cccgggcagc cgaggcgttc cgctcggaaa tggctgtcac ggccttttga
     4381 acatcctctt ccacaaaggc tccgagcgca agcagtttct ttttcaggtt atctatttcc
     4441 cgttggaggt gcccggacat ccctgtgctc cttcccaggc tggagctctg gtcgatttca
     4501 accgaacctg cctgtgatat aatcttcggt ctgcttcttc ttcggcatgg tgaatatctg
     4561 ctgcgtttca ccgaactcca cgaggtgccc ctcgtagaag aacgcagtga agtccgaaac
     4621 gcgcgctgcc tgctgcatat tgtgtgtgac aatcacgatc gtatagtcct cgcgcaattc
     4681 tcctatcaga tcctcgattc tcgccgtgga tcggggatca agcgccgacg cgggctcgtc
     4741 catcaacagg atttccggtc cgaccgcaag tgcgcgagcg atgcacagcc gttgttgctg
     4801 tcctccggag agcgaaagcg cattctgatc cagtcgatcc ttcacctctg cccagagacc
     4861 cgcccgttgg aggctctgct ctgcgatctc gcgaagtcgg gcgctgttct tttcgccgtg
     4921 gattcgcggc ccgtacacca cgttttcgaa gatggacttg ggaaagggat tggacttctg
     4981 gaacaccata ccgacgcttc ttcgaagtgc caccacgtcg gtcccgttcg agtaaatgtc
     5041 gtgtccgtca atgagaacgc ggccctgaac ccgggtcccc gggataatgt cgttcatgcg
     5101 attcaacgtc cgaaggaacg ttgacttccc gcacccggaa ggacctatga acgcggtcac
     5161 cttccggggt gccatctcga cgctgacatc aaaaagcgcc tgcaccgaac cgtaatgaaa
     5221 gtcgaggtgt tccacgacga ttttcgcatc agacgttcgt tcacccgtct ctaccatcgg
     5281 tacttcttcc tgaaacggcc tctcaaaaga atcgccgcca tgttgatgcc gagaaccagt
     5341 accaacagca ccagcgcggt gccgtactgc atccctcgaa ctttggcggc ttccgggtgt
     5401 tgagttgcaa gaatgtagag gtggtacggc aatgccatga cctggtcaaa cggcgactcg
     5461 ggaaggttcg gcaggaagaa ggccgccgct gtaagaagaa tcggcgcggt ttctcccgcc
     5521 gcccgcccga taccaaggat tgatccggtc atgattccag atacggaata cggaagaacc
     5581 gatctccaga tagtctgcca tctcgttgcg ccgagggcga ggctggcttc ccggagttcc
     5641 cgtgggactg cctgcaaggc ctcttcgctt gcgacgatga tcgtcgggag aatcatgcac
     5701 gcaagggtca agcttcccgc aagaacggaa gctccgaatc ccaggaaaat tacaaacaag
     5761 ccgagaccaa aaagaccgaa cacaacggac gggacccccg caagtgtgat tatcgcgaac
     5821 cgtacccccc gtgtgaacag cccttgtttc gcatattcgg aaaggtagac cgcactggcg
     5881 attccaagcg gcagagccgc ggcaatggcg ccggtgacga gatataccgt cccaactatg
     5941 gccggaaaga tcccgccttc agcgccgctc cgtctgggcg aatcgagaag gaacgagaga
     6001 ctgatggaag ggagtccctt ccagagtatg tccagaacaa tgatgcctac tgacgaaacg
     6061 agcaggtaga agatgatccg catcgcccag atcgcgagaa agccattgga gcgttgtctc
     6121 attgccgcac actgtaccgt ttgagtaccc tttgagcaac gatgttcaac aggaacgtgg
     6181 ccacaagaag gattatcccg acccagaaca acgcgcggta atgatcgctt ccgaacggga
     6241 cctcgcccat ttctgcggca atcgttgcag tcatcgtgcg taccgattcg aacggtttga
     6301 atgtggtctg cgcggcgttt ccggtaacca tcatcacagc cattgtctcg ccaatgacac
     6361 ggcccattcc cagcatcacc gcggcaacaa ttcccggcag cgcggcggga acggtgattc
     6421 gccagatcgt ctgaatctgg ttccccccta acgccagcga ggcctgtttg tacgaggccg
     6481 ggacactgcg caacgcgtct tcagaaaccg acatgatcgt aggaattgcc atgagcccga
     6541 gcagaagccc tccggtgaac gcgttcaggc ccgtttccag accaaatgct gcctttacga
     6601 acggagccag caccacgaga ccgaagaaac cgatcaccac ggacggaatt cctgcgagca
     6661 attcgatgaa gggtttgagc gtttccgttt ctgccggtct ggccacttcg gagagataca
     6721 ctgcgccgat gacgccgaaa ggaactgcca caaccgtcgc gattgcggtt accagtatgg
     6781 aaccggtgac caacggatag atgccgaact gctctttctg gaacgacacc ggtatccagc
     6841 gatcacccat gagcgaggaa agacccggat gctgcacaaa tggtgcggct tcccggaaaa
     6901 ggaagaggaa gatcagcccc acgagaatga tgcttgtgga agcggcaacc acgagtgtca
     6961 cctcgacaca gcgctccgtc cattttgtcc gcgggcgtgg catcacgatt tcctgagcga
     7021 caaaccggtt ggtttcgatg tcactggacg ggaacgtatc ctgtttccct gacgattctt
     7081 tgtccatccg gtcccaggca gaaatcaacg aactgtttgg ccgctcccgt gggaacaccg
     7141 tttgtgtaga aaaacagcgg acgcgcgatg gagtactcac ccgagtgcac agccgcctca
     7201 gtcggcatca ccgccgcacc ggcctcggtc gccttcaccc cgaggatctt aacctgtgga
     7261 cccgcctcat gggcataccc gatgccgaca tacccgatag accagcggtc aagaactaca
     7321 gactggatga tcgctgacgt tgccggcatc aaccgcaccg tttgcgcgaa atcttccttg
     7381 ttcaggacat gttcctgaaa aaacacgtac gtgccggaac tcgattcacg ggagagtgca
     7441 gtaatcggct cgttcgggcc gccaacctga ctccaccttg tgtacacccc cgtgtacatc
     7501 ttgcggagtt ggtcaaggga aagctcttga accggattcc tgggattcac cgcgatcgcg
     7561 attccatcgc gggccaccat ggtttccacc ggagtgatcc ccttctgctc tgcctgcgct
     7621 ctttccgtgt ccttgatatc ccggcttgag gcgcagatgt ccgtggtccc gttaatgagc
     7681 gcggctatcc ccgtgcccga accgccaccg gtagtggata tctccacatc ccggtgattc
     7741 ttcatgaacg cctccgccca catgcttacc aaatggacca ttgtgtccga ccccttcacc
     7801 gtcacgacgg tcacctcgtt tcctttcttc cccgaacatg ctgccagcat tccgaggaga
     7861 ccaatcgaga ttgcggtgag ggttcgtgtg gtaacgtttc tcatgtttca cctccatttc
     7921 ggcagttgtg ttcacaatga aggtcggccg ctccggtgag gattccgttt gaaccgggtt
     7981 aaggatttgt tagcgggatt gtaatggatg gtgctgtggg caccggagca aagggcggag
     8041 gtgggtcgcg gtgaacccac ctccattcct ggcgttgccc ggatccgtgc aattcgttct
     8101 ttgatggaaa tgcacgacga gcaaaacgcc taccgggcgg gaacgtaccc cgtttcccgg
     8161 actatcctct ggccttccga gctcaggcag aaatccacga accgtttggc ggctccggtc
     8221 ggagccccgt tcgtgtagaa gaagaggggc cgcgcgatgg aatactcccc cgaccttacg
     8281 gtcgcttcgc tgggcgtcac cgcagggccg gattcggtag ccttgatttt caacactttg
     8341 acgcgccggc ccgcgtcatg cgcgtacccg atgcctacgt accctattgc gccacgatca
     8401 gtcgaaacgg attgaataat ggcggaagtt gcgggcatca gccgaaccga ctgcgcgtaa
     8461 tcttgctgtt cgaggatgtg ctcctggaaa aaaacgtagg tacctgagct cgattcgcgc
     8521 gagagggcca gtattggtct gttcggtccc ccaacctgat tccattgcgt gtacaccgcg
     8581 gtgtaaatct tcttcaattg atcgtgcgaa agtgtgctca cgctgttgga ggggtgcacc
     8641 acgattgcga tgccatcgcg tgcaaccacc gtttcaaatg cggatcgtcc attttgctgt
     8701 gcccgtgcgc gctcggaatc cttgatgttc ctgcttgcgg cacagatgtc cgtcgtcccg
     8761 ttgatcagtg cggctatccc ggtgccggat cctccgccgg tgacagatat ctccacgtcg
     8821 ggattcgtcc gcatgaacgc ctccgcccaa gtgcttacca gatggaccat cgtatccgaa
     8881 cctttgatcg tcaacgacgt tctcgatccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn ngtgttggga ttgaggaacc agtcgagacc gagtgtgccg tccgtaagtg
     9061 ttccgccttt gatacccgag tcatccaggt cgagataaga gacgcgcgcc gcaactgcca
     9121 acgcacccca ctcgcttttt ctcgggtcga aattgcgcgt gggaactacg cgatcggcga
     9181 caccctgtcc gggtttgtac ggcctgtgat cgttcgtcaa gaaccatccc gcgtaggcgt
     9241 atccaccggt gaacgtcgcg ctgccgccac ctgttctggt cacacggctc ccgatccctt
     9301 ccgcctgcgc gaagaagcgc ccctgtgaaa cgagtgtttc caccccgaac agattgaccc
     9361 tctctgccgc gatggttggg gtgttgagga aattcggcgc aagattgact tcgggtttcc
     9421 ggctgtactt cacaacaccc ccgttgggat ttctggtgct gcccgagaac ccgaggtgaa
     9481 tgactcgttt ctgttctttc gaataggccg gcgcaaacac gacacgcccc gtgaaggcgt
     9541 attttccatc gccactgttt ttgccgtaat tgtccgaatc acggaagaca ccggcagccc
     9601 agcccgcacg cccggaggaa aacgtgccgc caagaagaag tccggcattg tacgccggtg
     9661 tgaagacgcc tgccagccca tattcgtgaa ataccaggta attgctgctc gtgatttcct
     9721 cgaggctgaa tggctcctgc atgtggccca gacgaagcgt tgacagcccc ggcacgccgc
     9781 ggagaccgat gtagacttct cggaaacttg cgtcgccacc gccgaagtcg aactcggccc
     9841 gccactcggt cgtgccgtac gcgattccat cgacgtgaat ccgggcgcgg cggaattcgg
     9901 taccatcctg caggtcacca aaccgcctgg tcagcgagtc ggattgggaa gtgaagaagg
     9961 tccagtcctg gtggatgcgt ccgccgacac ggatgcggaa ctccttgtcc gccgtctgca
    10021 gtaccagtcc cttatcccac gagggcgcga gggctccacc gtcagccgct ggagagggga
    10081 tggcagcggc cagcagaacg gcagcaacgc ctgccgcacc tacgctgaga accgaacgcg
    10141 ttccgcgaaa catacgtgct cctttttgag gggtgagact acggcgaaat cattttgcca
    10201 ttcaccggcg aatcctccga cccgcagtac agaaagtgcc cgagtccatt aagagaacgt
    10261 ttcgtaccgg tgagtttttt gcgaattttg agaaccaccc catcgatcgc actttcgcac
    10321 gggtgcggac gtttccgctc gtgggacgca tggtgtgcga tccgcaccgg tccgattgta
    10381 ttcccatcca acccgaaaag gggccctgca ccgtgatacc gtattcatta acccagcctc
    10441 aaccctttcg tgccagacgc ctgtcgagtt acgaccgcgc cggagggaac aacgactgga
    10501 tacccatcgg cggccggtct acgtgcgtca taggagacat ccgcggaccc ggaatcattt
    10561 cacatctgta ttttacggtg aacagccctg atccgcatta cctccgttct ctcgtcttga
    10621 gatgcttctg ggatgacgaa ccgttcccat ctgttgagtg tccgttgggc gatttcttct
    10681 gtgtggggca cggcgcggtc agcacgttcg aaaacgctgc cttcagtatg gccgcgaaca
    10741 aatcgaaaac atacggacgc ttcgccccga tgaattgctg ggtacccatg cctttttccg
    10801 aacgcgcgcg ccttgaaata acgaatgact cagatatccc tgtaatcgcc ttctatttcc
    10861 acatcgatta ccaggagtac gagcagttgc cgggaacacc atggtatctg cacgcgacat
    10921 ggcgccgtga gaacccctgc gacggatgga aagggaccgg ttcagtcgtc tgggggcccg
    10981 agtgggctgc acgacatcaa acggaagagg atagcaacct gacagggagt gggaattacg
    11041 ttatcctcga gacgagggga cgcggaacgt atctcggatg caatctatcg gtggacaatc
    11101 tctacaaagg gtggtggggc gagggcgacg acatgatttt cgtcgacggg gaggtgtggc
    11161 cgccttcctt gcacggcacc ggaacggagg aatacttcgg acagggctgg gggatgcagg
    11221 acgtccagca ccagtactac gggctatcgt atcacgagcg tgaggacttc aacgaccgcg
    11281 gcaaagtaac cgtctaccgg tttcacgttg ccgatccgat acctttccgc gagtcgatcc
    11341 gggtaacact ggagcacggg catgccaatg atcgttcgga cgactatgcc tcaaccgcgt
    11401 actggtacca ggccgaaccg cacgtgccgt tcgccgctct gccagatgtg ggtgctcgcc
    11461 tgccgaaccc gtgatttcac cagacggtgt acccgccgtc gatgagaatg ttcgcgcccg
    11521 tcatgaaact ggaggcgtcc gaagcaaggt agagtgcgag gggcccgagt tcctcaggcc
    11581 tgccgcaacg tttcatcggc gtgttctcga tccagacgtc gttctctcga ggattctgcc
    11641 ttcccctcaa ggtcatttcg gtttccatgt acccagggga gatcgtgttg acacgaatac
    11701 cgtactgcgc ccattcaccc gccaacgact ttgacaacat gatgacaccg gctttggcgg
    11761 cgttgtacga gcactgcggc tgtggatgat tcgacaccac accggacatg gacccgatgt
    11821 tgatgatgga accccgtttc cgcgcaatca tgtgtggcgc cacggcacgg caacatttga
    11881 acacccccgt cagatccacg gccagcacgt cttcccattg cgcgtccgtc atctcctccg
    11941 cggacgtgtt gatgacgatt ccggcgttgt tcatgagaac gtccacctgt ccgaattcgg
    12001 caatcgtgcg ttccaccatc gcgttcacag atgccgtgga acgaacgtcc agatggaccg
    12061 gcacggctcg atgcccggcg ctcacaaggt cgctcgcaac tcgttccgca ttggccagca
    12121 caacgtccgc aacaacaact gtggctcctg cgtccgcaaa cgtccggcag aacacttctc
    12181 caagtccgcg cccgcctccg gtgacaatcg ccacgcggcc atcaagctga aacactgtgt
    12241 tcgctgcaga cattgtcccg ttcctccccg attcaccgtt tccagtattc cgaatacgag
    12301 tccatcgccc gtacaacagc ttcgatattc tcgtacggtg tgtgagggta cacgccccag
    12361 atcagatgca ggccaccgcg aggcgaccca agagtcttga cacaccgtcc gatgtgccgt
    12421 tcgcattctt ccggcgtgcc gaatggcgta atgctctgcc ggtcgatatc aaggtcgagg
    12481 aatactctgc cccacgcaag ctcgcggagc gtctcaaggc cgttcacaag ctcctgaggg
    12541 ttcagaatcg aaacgccgca ctcgatcaga tcggggatga tatccacgat gtatccatca
    12601 gtgtgaagat gaacacgcac gccattcgag cgcagatact ggaatatccg catgtaggaa
    12661 ggtttgatga atgttcgcca cgcattcgga gaaatcggaa gcctgttctg gagccccagg
    12721 tcatcgccaa acgagacaag atccgctccg gcctcaacgt accgcttcgc aacgtcgaac
    12781 cagtaatcct cgacgacgcc tatgaggtcg aaaagccgct cgtcccgcga tccgacatcc
    12841 tccatgaacg cattgaatcc gcgaagatat gtcaggcgga gaaacacgaa gccgtggtca
    12901 gtgccgccac gtgccacctc acccctggcc ttcgcagcct tgaacttttt ttcaacctca
    12961 ctccaatccg tgaactccgc ggaatttggc cccttgtacg acgtcagcgc gtcccattcg
    13021 gcgacagggt gtcgcatcac aagcccgtcc agtgactcca gcggatacag ccactcgcaa
    13081 ccccacggat ccgtccattt gctgtacgcg ggcctgcttg gcgtgcgcga ggttcctacg
    13141 tgaacgtaac gggagaagcg ttccatccag tcgaggcgct ctttgtactc gttccacacc
    13201 ggataggtga tgccaacgtc ggagttgact gtcccatccc cgtcaaggtc cagctcgcgg
    13261 aagaatcttt cccgttccgt catctctttt ccgctcccgg tcaagggtgt gttgttgcac
    13321 ccgcagacac agcgtacatc ccaccggtta tagctatcaa gccgatgtac tgcacgaaag
    13381 aaaggttaga cttgaagatc agcgccgtag cgagttgcga cagcagaaac gcgcccccga
    13441 ctgccagccc catcgcaaca ttcacgttca tcgtcttgta cagctccatc atgaaccaga
    13501 tgcttgatgc gccaaacacg ttgccgataa tgaaccagag tgtccgcgac gcatccggca
    13561 ggctcccctg cttgaaagaa agcgccgcaa ccacctgcat cgcccagaaa atcaccaacc
    13621 agagcacgtt tgtcattgtc atcccgatcc actggtacgt tccacgccac cgatcaactt
    13681 gcgctaccga ttttcacctt ccgcacgcga aacgttggtg ttccgacgat cgcaccggaa
    13741 aaaacccacg tgaagtcgtc gccaactccg tcaac
//
LOCUS       DAOTNG010000043        13770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4360_length_13770_cov_4.719796, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000043 DAOTNG010000000
VERSION     DAOTNG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_13770_cov_4.719796"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="P5179_03450"
     CDS             complement(<1..447)
                     /locus_tag="P5179_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94307.1"
                     /translation="MAITHVRFSGTRREIGRTQGYRIRALRLPPASPRDIVFGNRCRA
                     VAARLHPGIEDEFEGMCEASGLPVEAFTSYYFGRTAPAAGGCTNIAVTGARTSNGDTL
                     VGRNYDWDYADRRWCEARTISPEGEPARIGYTHHWGGLCDVMNDAGL"
     gene            629..2719
                     /locus_tag="P5179_03455"
     CDS             629..2719
                     /locus_tag="P5179_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94308.1"
                     /translation="MALNRRWATVFRVLLAACAVRAGAAARLERGVLLVDGKPFFPLG
                     SWGGRDVLPEEIARLGMNATFLMAPSAEAALGSARAYMRECRRHGVQVMQYVPWGWDS
                     AAVRVAGQLAGEPNLLAWNVGDDVPLGGMEPLREVVRHLREAAPGTPTLADCIPSLGR
                     LDSVRRTFAEFVDITMNYDYPIPDAEYPLGGNAFRRHQAFFDEQRAMFGDPRWTWTQT
                     YLWHWTGQDLNVSPEAAGPYPEPEHVRLLVFSEINAGLRGLFCFSHREIALQPNLAAE
                     IAVTFREIALFNDFLAAGEIRRSLTTSDTTLDATAFSYRGSTLVSLALLRERYQYWIG
                     EGIARDVSVDVPWPGARLPNALLVATPDVIQCRVVRTDPGTVRVTIPSLELAGFVLVS
                     GDSRELARVRSRVAAIPREVRTLLVPGAMAQVQKVNQVVWQSGACDMQNTVISRAPVQ
                     AVERCSRAIDERRYADAVTEWKTANRLCRVALDSVMRYAGARAEQLTSDERRHLLTPY
                     GLHNIPALAFASPPGDRWRFVREWTVVGPFPLNRDWNDFDAVPEGFHTAYPPETSADR
                     HGVFDCIGGPAEWRYARSDLSGRLDFLQYFSPTENVLAYARCRVIVPRDTTVNFSIGS
                     NDGVKVWANGALAFELPVPRGRKAVRHQNEFPVSLHAGENRILVKLTNLGSNWQLYCA
                     VEDSAREFRFAPGW"
     gene            complement(2868..4217)
                     /gene="rseP"
                     /locus_tag="P5179_03460"
     CDS             complement(2868..4217)
                     /gene="rseP"
                     /locus_tag="P5179_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRS94309.1"
                     /translation="MSIQGTIAFLAALSVLVFVHELGHFLVAKWAGVRVETFSLFFGR
                     PLFSVRRAKGKGWQFRSLFWKWGLPDEAFEGDETEYAVRWIPFGGYVKMSGQSDFGDA
                     EDSGAPWEFTSKPVGARAAVIFAGPLMNFILAVIVFTWLNMTYGVVGRIGITPAPCMW
                     VGSVQAGSRADSLGVPVGARWISLNDSPLTSWKNVAQLLEKSTRPVIALQTPAGDTLR
                     IPMQKNLADLHEFGVEWENGAVIGGVVPIEPANEAGMREGDTVRAVNGQPVASWAEMS
                     RTIRRNAGRETVIEVKRDTITTVLTVTPSVDIGVDIPEGGVRLSFVPALRTAIVQTWV
                     VTVKIVKFIERLVTRAISPSYIAGPLGIFQMTGIAAEQGMGTYLMLVGTLSANLGFIN
                     LLPLAVLDGGLLVFLAFEAITKRRPSAKQQGYIQRIGMALLITMMILVTIMDIQRIF"
     gene            complement(4214..5389)
                     /locus_tag="P5179_03465"
     CDS             complement(4214..5389)
                     /locus_tag="P5179_03465"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRS94310.1"
                     /translation="MKRRLSILGSTGSIGRAALAIAARQPERFEVVALAAGNQWQLLV
                     EQIRDFRPALVSVSAESSAEEIRRALGDDCPSILSGSEGLEAVATFEPADIVVSSLTG
                     GVGVRPTYAAVCAGKDVALANKEAIVVAGEIIAREAASRGTRLLPVDSEISAIWQCLQ
                     AGRKQDVRRILLTASGGPFRSMPAEAFATITPEHALAHPTWVMGQKVTIDSATLMNKG
                     FEILEVRWLFDIPLDKIEVLIHHQSVIHSLVEYEDGSVIAQLGIPDMTLPIQYALTFP
                     ERSDTGRKPLSLADVGQLTFGRPDVARFPALALAREASRIGGTMPAVLSGADEVAVRA
                     FLSGQLPFAGIPRLVEDTMARHTAIPHPTLGDALSAESWAQETASSLLPGASKKGRS"
     gene            complement(5390..6235)
                     /locus_tag="P5179_03470"
     CDS             complement(5390..6235)
                     /locus_tag="P5179_03470"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRS94311.1"
                     /translation="MSLDRRNLALRILVAIIGIPLILFLLLTETWGVVALAAVLSYLG
                     AIEWADMTGLRKTPVLYGLAVVGTIALVVVGYIQRANLWSATVAAITVLAFVGTLVSR
                     WKEHGVVRPLGATIAGVVYVGFFALMIPIRRLQDGDALLVSLLFMIWLCDTAAYFGGS
                     LLGRHPLAPSISPKKSFEGAIFGFFGSIAGGVVGWLLFRPTTFIMAEFAFLGAAIGIV
                     GQVGDLAESAVKRETGIKDSSRLLPGHGGVLDRFDSLLFALPAVWFWLQTRAMLWGLV
                     VTPSF"
     gene            complement(6232..6999)
                     /locus_tag="P5179_03475"
     CDS             complement(6232..6999)
                     /locus_tag="P5179_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRS94312.1"
                     /translation="MTDAEQQAALKARGNLPRHIAVIMDGNGRWAKQNGFSRITGHRH
                     GVESVRELVRASAELGIEVLSLYVFSTENWNRPRHEVIGLMRLLRQTILRETDELNQN
                     NVRLRASGRIDDLPPAAAKALREAITTTGKNTGLILNLCINYSGKAELVDAIREIVRS
                     GIPAEAITPETVAGYLYTADLPEPDLLIRTSGEMRLSNFLLWQTAYTELWFTPVLWPD
                     FRRPHLYEAISAFQQRQRRFGKTQDQLFVEPREIGPE"
     gene            complement(7096..9189)
                     /locus_tag="P5179_03480"
     CDS             complement(7096..9189)
                     /locus_tag="P5179_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRS94313.1"
                     /translation="MTFLNPMFLFALAAAGIPVAIHFLGKRRPRRVAFSAMVFLEEVR
                     RQQFRRLKFRNALLLLLRTATIVCVVLAFARPTIKTNAGAFGHEGASAIVVVLDDGPG
                     MGYESSYGRRIDWARRQLSDMLSVVRPGDWAVLIRTSRPDLVLPFTRATLENLKSVSD
                     WSGDGVAALQHAADILGRSDAANREVYVVSDLAGPPWQSLRPVQWPDRTAGYLLAPAD
                     EPATNLAVDSVAAGNQFVRAGRPFSVRAIVRNTGTVDVNDASADLWFDNRRMQRQVVS
                     VPAGGVAAVTFSAVVPAPGWLTGRIELSDDPLIADNTRHFAVFVPAETRLAIIGDESP
                     SRAFLSALFTAADSTNPYSVAVGTERTIPRDSVEQADVIIVNERVPAGANVLGWISEA
                     THRASAQSTARDGAGILFLTGPGTDLTAANQSLLPLFTGDRFAGLSTLSAGTGGYYSL
                     DSRSVSDPVLTSLLSSSARASQPRFFTFARLTGGAAHRAVSFTTGDPWLVRGEKEPVR
                     GMVVTAGLSPEWGDLAYRGVVVPLMHHLVHSLARTSPVAGEYPAGASVRRPLVTAGET
                     IMLTAPDGLQRRVVPAGDEIPSLDLGVLTPRGCWSISADGNVTDMIAVNLHRDESELQ
                     RLSSGAFAGAVGLRSLTRLGARPADDIISARIGTELAVPFFLAAMACIAAELLLMRGI
                     AMGKRDAEAAAPPSV"
     gene            9376..11019
                     /locus_tag="P5179_03485"
     CDS             9376..11019
                     /locus_tag="P5179_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRS94314.1"
                     /translation="MRAVIAALLFLVVSHAVAQTDTRVALGVLDLDSGTIPEAELRVL
                     SDRLRVELFNTGQFRIIERERIDAVLREQGFQLSGCTATSCVVEIGELVGVRKMVAGS
                     VGKVGDVYSLTLRIIDVESGAIERTAVQDCRCSLQQLLTSTIALAAAELAGVAPTVTQ
                     TLERGEGYGVLYVNSIPPGAVIYLNGTLRRERTPATIDRLPAGEHIVRAMLDSLVGEQ
                     RVRVTRDAVTKTTLSLQPGTGSLFITSEPPEASVTLDGRPAGTTPLLLPQVTVGTRTV
                     VFSHADFFPDSQRVTVRFNERTPVSARLVPCGYLDIQVTPATARVYLDSRELGRDQMR
                     LRLPLGQYRLTMVAADYDTVRRAVTIGHGQTVVVRETLTSVFGGVRITSNPEGASVSC
                     PAAGIRGFTPFESDRLLPGSYRVRVEKAGRLPGEVNVAVIKGRTVSIHVNLAEDPDIV
                     RMRNERIKVRARWTTLVVGLAAAGFGAKWGFDANHAYGDRETAYTNYLSATTTATATQ
                     WRTAYENADRRGDRAAKKRNVSLGAAGALLGVSIVLTVR"
     gene            11152..11766
                     /locus_tag="P5179_03490"
     CDS             11152..11766
                     /locus_tag="P5179_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS94315.1"
                     /translation="MEIDVDKPVRRNTRLAGFDYTQAGAYFITVVTRRRVCVFGDVVD
                     GEMRVNPAGEMVDAVWRELPAHYPGVKIDWHVVMPNHFHGIVVLTDANERAVGAGPRA
                     RPQPNADENGRPQGVAPTMGLSDVLQRFKSLTTNEYIRRVASDGWPPFSRHLWQRSFH
                     DHIIRDDDDLARVREYIALNPVRWADDEENLANAREHERRGGKG"
     gene            11876..13174
                     /locus_tag="P5179_03495"
     CDS             11876..13174
                     /locus_tag="P5179_03495"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS94316.1"
                     /translation="MRTGKWVLLTCVAAGTLVTCSKDDHVLNNPADPQSPAYVGHEVL
                     SPHDPANFPTVAITSAPSEAKVGTAVTFVATASDPNPKGLRTGNLRSVSWNFGDGSAI
                     VQGGLTQSHTFSAAGLFYVGITVVDDDGNSANAAKVISITRGLPPTASAGGPYSCLRN
                     TPLTLNGGGSDPDGYIVKYEWDFDGNGSFDWSSVTTGSVERVFTVAGMQAPVLRVTDN
                     DGNAATASASVAVAYLGDFESSSQLGDFTVNTARGTFAVTAGTMRIQCTDTLYIAEAK
                     LKNATFANGTLEVTTQWVSGVNNYSYGLWFRGTNSNEYGFAITRNGWYTIVKWTNGQW
                     GTLGTWTSSGAITTGANVLKVVCNGPNMSFFINGTMATAVSDASYASGSVALWVGGQQ
                     TVLFDDFRMTPTAGTSARVARPASAPEGELPARAVAPQAP"
BASE COUNT         2473 a         4144 c         4690 g         2463 t
ORIGIN      
        1 gagcccggcg tcgttcatga cgtcgcacag gcctccccag tggtgcgtgt acccaatgcg
       61 cgcgggttcg ccctccggcg aaatagtccg cgcctcgcac cagcggcgat ctgcgtagtc
      121 ccagtcgtag ttccgtccga cgagcgtgtc accgttgctc gttctggcgc cggttaccgc
      181 gatgttggta cagccgccgg cggcaggcgc ggttcggccg aagtagtagc tggtgaaggc
      241 ctccaccggc aagccgctgg cctcgcacat gccttcgaac tcgtcttcga taccgggatg
      301 caggcgcgcg gcgacggcgc ggcaccggtt gccgaacaca atatcgcggg gtgaggcggg
      361 cggcagcctg agggcgcgga tacggtaccc ctgcgttctg ccgatctccc ggcgcgtgcc
      421 ggagaaacgc acgtgtgtga tcgccatggg gtcgtctccc tgttgtccgc ggcggcacat
      481 ctgcgattcc gctcatctgc cgcgaatgaa atagaacggg aattccgttc cgggaaaaga
      541 ggagggaacg gttgcccccg gaaccacgcc accgtcagtt tggttagggc tgcggcccca
      601 tagtgaattc ttcgcgggaa ggtgatgaat ggcgctgaac aggcgatggg cgacggtttt
      661 cagagttctt ctggccgcgt gcgcggtccg agcgggcgcg gcggcccgcc ttgaacgcgg
      721 tgtgcttctc gtggacggga agccgttctt ccctctgggc agctggggcg gcagggatgt
      781 tttgcccgag gaaatcgccc ggctgggcat gaacgccacg tttttgatgg cgccttcggc
      841 ggaagcggcc cttggcagtg cgcgggcgta catgcgagag tgcaggcggc acggcgtgca
      901 ggtgatgcag tacgtcccgt ggggatggga ttccgccgcg gtgcgcgttg cggggcagct
      961 cgccggcgaa ccgaatctgc tcgcgtggaa cgttggcgat gacgtgccgc tcgggggcat
     1021 ggagccgctg cgcgaagtgg tccggcacct tcgggaggcg gccccgggaa cccccaccct
     1081 ggcggactgc attccttcgc tcggacgtct cgactctgtc aggcgcacgt tcgccgagtt
     1141 cgttgacatc acgatgaact acgactaccc gattcccgac gcggaatacc cgcttggtgg
     1201 caacgcattc cggcggcacc aggcgttttt cgatgagcag cgagccatgt tcggcgaccc
     1261 acggtggacg tggacgcaga cctacctctg gcactggacg gggcaggacc tgaatgtgag
     1321 cccggaagcc gcagggccct acccggagcc ggagcatgtg cgcctgcttg tcttttcgga
     1381 aatcaacgcc ggtctgcgcg ggttgttctg tttttcgcac cgggagattg ccctccagcc
     1441 gaaccttgcg gcagagatcg ccgtaacgtt ccgggagatt gcgctcttca acgatttcct
     1501 tgcggcaggg gaaatccggc gttctctcac cacatccgac acgacgctcg acgcaacggc
     1561 gttttcgtac cgagggtcaa cgctggtctc gctggcgctc ctgcgggaac ggtatcagta
     1621 ctggataggc gagggaatcg cacgggacgt gtcggtggac gtgccgtggc ccggtgcccg
     1681 tctgccgaac gcactgcttg tggcgacgcc tgatgtcatc cagtgccggg tggtcaggac
     1741 ggatccggga accgttcgcg tcaccatccc gtcgctggaa ctggcgggtt tcgtgctcgt
     1801 gtccggtgat tcgcgcgaac tggcccgggt gcggagccgc gtggccgcaa tcccccgcga
     1861 ggtgcgcacg cttctggttc ccggtgcgat ggcgcaggtg cagaaggtga accaggtcgt
     1921 gtggcaatct ggcgcgtgcg acatgcagaa cacggtcatc agccgggctc cggtgcaagc
     1981 agtggagcgg tgcagccggg cgattgacga gcggcgctac gcggacgcag tgacagaatg
     2041 gaaaacggcg aaccgtctgt gtcgtgtggc gctggacagt gtcatgcggt acgccggcgc
     2101 gcgggcggag cagttgacat cggacgagcg gcggcacctg ctcacgccgt atggcctgca
     2161 caacatcccg gcgctggcgt tcgcttcccc gccgggcgac cggtggcggt ttgtgcgcga
     2221 atggacggtg gtcgggccgt ttccgctcaa ccgcgactgg aacgatttcg atgcagtgcc
     2281 ggaagggttc cacacggcct atccacctga aaccagcgcc gaccgtcacg gtgtgttcga
     2341 ttgcatcggc ggccctgcgg agtggcgtta tgcgaggagc gatctgtccg gaaggctgga
     2401 tttcctgcag tacttcagcc cgacggagaa cgttctcgcc tatgcccggt gccgagtgat
     2461 cgttccgcgc gacaccaccg tgaacttcag catcggcagc aacgacggcg tcaaggtgtg
     2521 ggcgaacggt gcgcttgcgt tcgagctgcc ggttccccgc ggcaggaaag ccgttcggca
     2581 tcagaacgag tttccggtct cgctccacgc cggggagaac cggattctgg tgaagctgac
     2641 gaatcttggc tccaactggc agttgtactg cgcggtggag gactctgcgc gggaattccg
     2701 attcgcgccg gggtggtgag gcggcggcgg cgggcgtacg tcacatcgga cgtacaggac
     2761 ctgtaaggag ggctccgcac tgggatccgg cgccatgaga aaggggtagg tctgcctttc
     2821 ccacaaggca ggcctacccc aaaagaagtc ggctacaggc cgacttctca gaagatgcgc
     2881 tggatgtcca tgatggtgac gaggatcatc atggtgatca gcagcgccat gccgatgcgc
     2941 tggatgtagc cctgctgttt cgcgcttggc cgccgcttcg tgattgcctc gaacgcaaga
     3001 aacacgagga ggccgccatc cagcacggca agggggagca ggttgatgaa cccgaggttg
     3061 gcgctgaggg ttcccaccag catcaggtag gtgcccattc cctgttccgc ggcgattccg
     3121 gtcatctgga aaatccccag cgggccggca atgtagctcg gggaaatggc acgcgtcacc
     3181 agccgttcga tgaatttgac gattttgacg gtgactaccc acgtctgaac gatggcggtt
     3241 ctaagcgcgg gcacaaaaga gagcctcacg cctccttcgg gaatgtccac gccgatatca
     3301 acggacgggg tgacggtaag caccgtggtg atggtatccc gcttcacctc gatgacggtc
     3361 tctcttcccg cgttgcggcg gatggtccgg ctcatttcgg cccacgatgc caccggctgg
     3421 ccattcaccg cgcggacggt gtcgccttcg cgcatgccgg cctcgtttgc cggttcgatc
     3481 ggcacgacgc cgccgattac cgcaccgttt tcccattcaa caccgaactc gtggaggtcg
     3541 gccaggttct tctgcatggg gatgcggagc gtatcgccgg cgggcgtttg aagcgcgatt
     3601 acgggacggg tgctcttttc gaggagttgc gccacgtttt tccagctcgt gagcggcgaa
     3661 tcgttgagcg agatccagcg cgctccgacg ggaacgccga ggctgtcggc gcgtgaaccg
     3721 gcttgaacgg agccaaccca catgcagggg gcaggcgtta tgccgatgcg cccgacgacg
     3781 ccgtaggtca tgttgagcca ggtgaacacg atgacggcga ggatgaagtt catgaggggg
     3841 ccggcgaaga tcaccgcggc acgggctccc acgggtttgg aggtgaactc ccagggtgcc
     3901 ccggaatcct cggcgtcgcc gaaatcgctc tgtccgctca tcttcacgta gccgccgaag
     3961 ggaatccagc ggactgcgta ttccgtttcg tcaccttcga acgcctcgtc cggtaatccc
     4021 catttccaga agagagagcg gaactgccag ccttttcctt tcgccctgcg aaccgaaaag
     4081 agcggtctgc cgaagaacag ggagaaggtt tcgacgcgaa cgcccgccca tttggccacg
     4141 aggaagtgac ccagttcgtg gacaaacacg agcacgctga gcgcggcgag gaaggcgata
     4201 gtgccctgaa tgctcatgag cggcctttct ttgaggcacc gggaagcagg gaagacgcgg
     4261 tttcctgtgc ccacgattcc gcggaaaggg catcgcccag cgtggggtgc ggaatggcgg
     4321 tgtggcgtgc catggtgtct tcgaccaggc gcgggatgcc ggcgaagggc aactggccgg
     4381 aaaggaaggc gcgcacggcc acctcatcgg ccccgctgag taccgccggc atcgtgccgc
     4441 cgatccgcga cgcttcgcgg gcgagtgcga gcgcagggaa gcgggcgacg tcgggtctgc
     4501 caaacgtcaa ctgcccgacg tccgcaaggc tgagtggttt ccttcccgta tccgagcgct
     4561 ccgggaaggt aagcgcgtac tgaatcggca gcgtcatatc gggaatgccg agttgcgcga
     4621 taaccgaccc gtcctcgtac tccaccagcg aatggatgac ggactggtga tggatcagga
     4681 cctcgatttt gtccagcggg atgtcgaaca gccagcgaac ttcgaggatc tcgaagccct
     4741 tgttcatcag cgttgccgag tcgatggtca ctttctgccc cattacccag gtggggtgcg
     4801 caagggcatg ctccggagta atggtggcga atgcttccgc cggcattgac cggaaggggc
     4861 cgccggacgc agtgaggagg atgcgccgga catcctgttt ccggcccgcc tgaaggcact
     4921 gccagatggc acttatctca ctgtcaacgg gaaggaggcg ggtgccgcgg gaggcggctt
     4981 cccgggcgat gatttccccg gccaccacga tggcctcttt gttggccagg gcgacgtcct
     5041 tgcccgcgca cacggcggca taggtggggc ggacgccaac gccgccggtg aggctgctga
     5101 cgacgatgtc ggcgggttcg aacgtggcaa ccgcttcaag gccttcgctg ccggagagta
     5161 ttgaggggca gtcgtccccc agtgcgcggc gaatctcttc cgctgacgat tccgcggata
     5221 cggagaccag tgccggccgg aaatcgcgga tttgctccac gagcagttgc cactgattcc
     5281 cggcggccag cgcgactacc tcaaaacgct ccggttgccg cgccgcgatt gccagcgccg
     5341 cccggccaat ggaccccgtg gaaccgagta tggagaggcg tcttttcatt cagaaagaag
     5401 gggtgactac gaggccccag agcatggccc gggtctgcag ccagaaccag acggcgggca
     5461 acgcgaacag gaggctgtcg aacctgtcga gaacgcctcc gtggccgggc aggaggcgtg
     5521 aggaatcctt gatgccggtt tcccgcttga cggctgactc cgcgaggtct cccacctgtc
     5581 cgacgattcc gattgcggcg cccaggaacg cgaactccgc catgatgaag gtggtcggcc
     5641 ggaacagcag ccaacccacg actccgccgg cgatggagcc gaagaagccg aatatggctc
     5701 cctcaaaaga cttcttcggg ctgattgagg gcgcgagagg gtgccggccc agcaacgacc
     5761 cgccgaagta tgccgccgta tcgcacagcc agatcatgaa gagcagggag acgagaagtg
     5821 cgtcgccatc ctgcagacgg cggatcggta tcatcagggc aaagaaaccg acgtacacca
     5881 cccctgcgat tgtggcgccg agcggacgga ctacaccgtg ttccttccag cggctcacaa
     5941 gcgttcccac gaacgccaga accgtgatgg cggcgacggt tgcgctccag aggttcgccc
     6001 tctggatata ccccaccacc acgagggcga ttgtgccgac aaccgcgagc ccgtacagca
     6061 caggggtctt ccgcagcccg gtcatgtcgg cccattcgat cgcgccgagg taactgagga
     6121 ccgcggcgag cgccaccacg ccccaggttt ccgtaagaag cagaaagaga atgagcggaa
     6181 taccgatgat tgccacgagg atgcgcagcg cgagattcct tctgtcaagg ctcattcggg
     6241 gccgatctcc cggggttcca cgaaaagttg gtcctgtgtt ttgccaaacc gccgctgccg
     6301 ctgctgaaac gcgctgatgg cttcgtacag gtgcgggcgg cggaaatccg gccagagcac
     6361 cggcgtgaac cagagttcgg tatacgcggt ctgccagagg aggaaattgc tgaggcgcat
     6421 ttcgccgctg gtgcgaatca acaggtccgg ctccggcaga tcagccgtgt agaggtaccc
     6481 ggcgaccgtt tccggagtga tcgcctcggc aggaattccg cttcggacga tctccctgat
     6541 ggcgtccacg agttccgcct tgccggagta gttgatgcac aggttcagaa tgagtcccgt
     6601 gtttttcccg gtcgtcgtga ttgcttcccg cagggccttc gccgcggcgg gcgggaggtc
     6661 atcaatccgg ccggaggcac gcaggcggac gttgttctga ttcagttcgt ccgtttcccg
     6721 caggatggtc tgcctgagaa ggcgcatcag cccgatgact tcgtgccggg ggcggttcca
     6781 gttttccgtg gagaacacgt agagcgacag cacttcgatg ccaagctccg cggaggcgcg
     6841 caccagctcg cgcacggatt ccactccgtg gcggtggccg gtgatccggg agaagccatt
     6901 ctgtttcgcc catcgcccgt tgccgtccat gatgacggcg atgtgccggg ggaggtttcc
     6961 gcgcgctttc agggcggcct gctgttcggc gtctgtcaac gtgagatcgc gctcctcata
     7021 aaggcgggac acgcaactga gatgcaacct gcagaacagg gttaactaca taatatcagt
     7081 ttttggcggg cgcattcaaa ctgatggagg cgctgcggct tcggcgtcac gtttccccat
     7141 cgcgatgccg cgcatgagca ggagctccgc cgcgatgcat gccatggcgg cgaggaaaaa
     7201 cggcaccgcg agctccgtgc cgatgcgcgc cgagatgatg tcgtccgccg gtcgggcgcc
     7261 cagtctggta agcgatctga gtccgacggc gcccgcgaag gctcccgagg aaagccgctg
     7321 gagttccgat tcatcgcggt gcaggttgac cgcgatcatg tcggttacgt tcccgtcagc
     7381 ggagatgctc caacacccgc ggggagtcag aacgccgaga tcgagggagg gaatctcgtc
     7441 gccggcaggc accacccgtc tctggaggcc atccggagcc gtgagcatta ttgtttcccc
     7501 ggcggtgacg agcggccgtc tcacggaggc tcccgccggg tattcccccg caaccgggga
     7561 cgtgcgcgcc agcgagtgca cgaggtggtg catcagggga accaccacgc cgcggtaggc
     7621 cagatctccc cactccggag aaaggccggc ggtgacaacc atgccccgga cgggctcctt
     7681 ttcaccgcgc accagccacg ggtcgcccgt ggtgaacgat accgcgcggt gggccgcgcc
     7741 tcccgtcaga cgggcgaagg tgaaaaaccg cggctgactc gctctggcgg aggagctcag
     7801 caggctggtc aggacggggt cggatacgct gcgggaatcc agcgagtagt acccgccggt
     7861 gcccgcggaa agggtggaaa gccctgcgaa ccggtcgccc gtgaacaggg gcagcaatga
     7921 ctgattcgcg gcggtgaggt cggttccggg ccccgtgagg aagaggatac cggcgccgtc
     7981 gcgggcggtt gactgcgccg acgcgcggtg cgtggcttcg gaaatccatc cgagcacatt
     8041 cgcgccggcg ggcacgcgtt cgttcacgat gattacatcc gcctgctcga cggaatcgcg
     8101 gggaatggtg cgctcggtgc cgacggctac ggagtacggg tttgtcgaat cggcggcggt
     8161 gaaaagggcg gagaggaatg cgcgtgaggg cgattcatca ccgatgatgg cgaggcgggt
     8221 ttccgccggc acgaataccg cgaaatggcg ggtgttgtcg gcgatgagcg ggtcgtcgga
     8281 aagctcgatt ctgccggtca gccatcccgg cgccggcacg acggcggaga atgtgacggc
     8341 ggcaacaccc ccggcgggaa ccgacacgac ctgccgctgc atgcgccggt tgtcgaacca
     8401 gagatccgcg ctcgcatcgt tgacgtctac cgttccggta ttcctgacaa tggcgcgcac
     8461 ggagaagggc cggcccgcgc ggacgaactg gtttcccgcc gccaccgaat ccaccgcgag
     8521 gttcgtagca ggttcatccg ccggcgcgag caggtaccct gcggtgcggt caggccactg
     8581 cacagggcgg agcgactgcc acggtgggcc ggcgaggtcg ctgacgacgt acacctcgcg
     8641 attggccgcg tcgcttcgtc cgaggatatc cgcggcgtgc tggagggcag ccacgccgtc
     8701 accggaccaa tcggacacgg atttcaggtt ctccagcgtc gcgcgggtga aagggagcac
     8761 gaggtcgggc cgggaggtgc ggataagcac cgcccagtcg ccggggcgca cgacggagag
     8821 catatcgctg agctggcggc gtgcccagtc aatccgcctg ccgtatgaac tttcgtaccc
     8881 catgccgggg ccgtcatcca gcaccaccac aatggcgctt gcgccttcgt ggccaaacgc
     8941 gccggcgtta gttttgatcg tcgggcgcgc gaacgccagc acgacacaca cgatggtggc
     9001 ggtgcggagc agaaggagca gcgcgttgcg gaacttgagc cgccggaact gctgccggcg
     9061 cacttcctcg aggaacacca tggcgctgaa cgccacgcgg cggggccggc gtttcccgag
     9121 gaaatggatt gccaccggga tgccggcggc agcgagggcg aacaggaaca tcgggttgag
     9181 aaacgtcatg ttgggaatgt acgaactgcg tgcgtcaacg ccgatacttg aggcgtggtg
     9241 gggacaccga cgggtgtgag aagccagccg cgcacggagt cgattgcgtt tgactgctcc
     9301 gctgcatttc cgtaccgttc acatgcacaa gaggtgcggc gcgatacggc aaaggcgctt
     9361 gacgaggagg agttcatgcg cgcggtgatc gctgctctcc tgtttctggt tgtctcacat
     9421 gcggtggcgc agaccgacac gcgggtagcc ctcggcgttc tcgatctgga cagcggcacg
     9481 attccggaag cggaactccg tgtgctttcc gaccgcctcc gcgtggagct gttcaatacc
     9541 ggccagttcc gcattatcga acgtgaacgc attgacgcgg tgctgcggga gcagggtttc
     9601 cagctttccg ggtgcacggc cacgtcctgc gtggtggaaa tcggcgaact ggtcggcgtg
     9661 agaaagatgg tggcgggttc cgtcggcaag gtgggcgacg tgtactcgct tacgctccgc
     9721 atcattgacg tggaatccgg cgcaatcgag cgcaccgctg tacaagactg ccgctgctcg
     9781 cttcagcagc ttctcacctc caccatcgcg cttgccgcgg cggagcttgc gggtgtggcg
     9841 ccgacagtca cgcagacact cgagcggggg gagggatacg gcgttctgta cgtgaattcc
     9901 atcccgcccg gcgcggtgat ctacctcaac ggcacgctgc gacgcgagag gacgccggcc
     9961 accattgacc gtctgcccgc cggtgaacac atcgtgcggg cgatgctgga cagccttgtg
    10021 ggcgaacagc gggtgcgggt gacgcgggat gcggttacga aaaccacgct ttcgctccag
    10081 cccggcacag gcagcctgtt catcacaagc gagccgccgg aagcctccgt aacgctggac
    10141 gggcgccctg caggaacaac gccattgctg cttccgcagg tgaccgtcgg cacgcgcacc
    10201 gtggtctttt cgcacgccga tttcttcccc gattcgcagc gcgtcaccgt tcgtttcaac
    10261 gagcgcaccc cggtttcggc aaggcttgtc ccgtgcggtt atctggatat ccaggttact
    10321 cccgccacgg cgcgggtata cctcgactcg cgcgagctgg ggcgggacca gatgcgcctt
    10381 cggctgcccc tcggccagta ccgcctcacc atggttgccg cggactacga caccgtgcgc
    10441 cgggcggtca ccatcggcca cgggcagacc gtggtggtgc gggagacgct cacgagcgtg
    10501 ttcggcgggg ttcgaatcac ctcgaatccg gagggcgctt cggtttcgtg ccctgcggcg
    10561 gggattcgcg gtttcacacc gtttgaaagc gaccgattac tgcccggttc gtaccgcgtc
    10621 cgtgtggaga aagcggggcg gttgccgggt gaggtgaacg tcgcggtgat caaagggaga
    10681 acggtatcca tccatgtgaa tctggcggaa gatcctgata tcgtgcgcat gcggaacgag
    10741 cggatcaaag tgcgggcgcg atggacaacg cttgtcgtcg gcctcgcagc cgccggcttt
    10801 ggcgcgaaat ggggttttga cgcgaaccac gcgtacggtg accgcgaaac agcctatacg
    10861 aactacctga gcgcaaccac cactgccacc gcaacgcagt ggcgaacagc gtacgagaac
    10921 gcggaccggc ggggcgaccg cgcggcgaag aagcgcaacg tctctcttgg ggcggcgggg
    10981 gcgctgctgg gggtgtcaat cgtgttgacg gtgcggtgag agggtttttc cctcaccccc
    11041 gaagctgcgc cacgcgcagc accctctccc ggtgggcgag gggaacaaag gagtgttgcc
    11101 cttccgacgg caggggaaag gcagcgttgg cggtgtgggg cgaggatttc gatggagatt
    11161 gacgtcgaca aaccggtccg ccggaatacc cggttggccg ggttcgatta cacacaggcc
    11221 ggggcgtatt tcatcacggt cgtcacgcgg cgccgcgtgt gcgtgttcgg cgatgtggtg
    11281 gacggggaga tgcgggtgaa tccggcgggc gagatggtgg atgcggtatg gcgggaattg
    11341 ccggcgcatt atccgggtgt gaagattgat tggcacgtgg tcatgccgaa tcattttcac
    11401 gggattgttg tgttgacgga tgcgaacgaa cgtgcggtag gggcgggccc ccgtgcccgc
    11461 ccgcaaccca acgcagacga aaacgggcga ccacaggggg tcgcccctac gatggggttg
    11521 tcggatgtcc ttcaaaggtt caaatctctc accaccaacg aatacatacg ccgcgtggcg
    11581 tctgacggat ggccgccgtt ttcccggcat ctctggcaac gttcatttca cgaccacatc
    11641 atccgggatg acgacgacct cgcccgggtg cgggaataca tcgcgttgaa ccccgtgcgg
    11701 tgggccgacg acgaggaaaa cctcgccaac gcaagggaac atgaacggcg cggtggcaaa
    11761 gggtagggga gcacctgtgt gtgcgcccgt tcttgtgtga aaggtatccg ggcggacacg
    11821 caggtccgcc cctacgcata tttcgacatt cgttcaccac gaaggaggcc acccgatgcg
    11881 tacggggaaa tgggtgcttc tgacatgcgt cgcggcggga acgctggtca cctgctcgaa
    11941 ggacgaccac gtcctgaaca accccgccga cccgcagagc cctgcgtacg tggggcacga
    12001 ggtactctct ccgcacgacc cggcgaactt cccgaccgtc gccataacca gtgcacccag
    12061 tgaagcaaag gtcggcactg cggtgacatt cgtggcgaca gcaagcgacc ctaatccgaa
    12121 gggactgagg accggaaacc tgcggagcgt ctcgtggaat ttcggggacg gttccgcgat
    12181 cgttcagggc ggtttgactc agtcgcacac attttccgct gcgggattgt tctatgtggg
    12241 gataacagtg gtggatgacg acggcaactc ggcaaacgcg gcgaaggtca tcagcataac
    12301 ccgtgggttg cccccgaccg cgtccgcagg cgggccgtac tcttgtcttc gaaacacgcc
    12361 gttgacgttg aatggcggcg ggagtgaccc ggatggctac atcgtcaaat acgaatggga
    12421 tttcgatgga aacgggagct tcgattggtc atcggtaaca accggttctg tggagcgcgt
    12481 tttcactgtg gcgggaatgc aggcaccggt gctgagggta acggataacg acgggaacgc
    12541 tgccacggct tccgcctccg tcgcggtcgc gtatctgggc gattttgaat catcctctca
    12601 actcggcgac ttcactgtga acacagcccg gggaacattc gccgtaactg cggggacgat
    12661 gcgtatccag tgtaccgata cgctgtacat cgcagaagcc aaactgaaga acgccacgtt
    12721 tgcgaatggc acgctggaag tcacgacgca atgggtttcc ggggtgaaca attactcgta
    12781 cgggctttgg tttcgaggta ccaacagcaa cgaatacggt ttcgccatta cccggaatgg
    12841 gtggtacacg atagtgaaat ggacgaacgg gcagtgggga acgctcggaa cttggaccag
    12901 ttcgggcgca ataacgaccg gcgcgaacgt cttgaaagtg gtgtgcaacg ggcccaatat
    12961 gtcatttttc atcaacggga ccatggccac ggccgtttcg gacgcctcct atgcctcggg
    13021 atcagtagcc ttgtgggtag gtggccagca aaccgttctt ttcgacgatt tccgcatgac
    13081 accgacggcg ggtacgagcg ctcgggttgc cagaccggcg agcgcaccgg aaggggaatt
    13141 gcccgcccgc gcagttgcac cgcaagcgcc gtaggggcgg gttgcccggc ccgttgccgc
    13201 cgtcggtgcg tgcccacccg cgttggtggt cggctcccgc gggccgacac aggggtcggc
    13261 ccctacgccg ttttctgtgg cggaggaggg ttgcccggcc cgggtttgtg cgcgggttgg
    13321 cccccgcggg tgtgatgttt tggggtaggg gcagacctgt gtgtctgccc gccgaacgcg
    13381 agaaaaacca cgggcggaca cgcgggtccg cccctgccgg attgctgttc gtgcgccgtt
    13441 gggggtcggg tcgcccgacc cgttgctgcc ttgcgtgtgt gccggcgcgg gcggggaaga
    13501 ccctgcgtgc gcgccgcgcg cgacgcccct acgccgattt cgttgccggc tgaccccctc
    13561 ttcgcgtcct tcgcgttctt cgtgtgcgcc attgttctcc cctccccaaa aaacgcgtac
    13621 cgcgattgaa ccattttcgc gaaaaacctt gacacgagga cgccgcgtcg cctgtgttgt
    13681 acgtgtggcg gcagcgttcc cggggacggg tcggtgggcc ggcggtcggc agatgaagga
    13741 aaaggagcgt gtgatgcgtg aagcgccggc
//
LOCUS       DAOTNG010000044        13738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4372_length_13738_cov_5.665863, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000044 DAOTNG010000000
VERSION     DAOTNG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4372_length_13738_cov_5.665863"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>245)
                     /locus_tag="P5179_03500"
     CDS             complement(<1..>245)
                     /locus_tag="P5179_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94317.1"
                     /translation="TGRRLAVRWNRPQAEQTTLDLSYREPWVAGFPVDAEGKLAVEQR
                     TAYALERLELAVGGELVPDVMVAASIGREIVRSDSVP"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<256..1128)
                     /locus_tag="P5179_03505"
     CDS             complement(<256..1128)
                     /locus_tag="P5179_03505"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRS94318.1"
                     /translation="MIRRIAALATGACAVFCCAKVCPADAVQTDSVRVVVRGARAFPA
                     DRIRLWASGPDGLRRIGKEYSDAGFWSAILQDTLYVSRNGDAVREILVHEGDIASVTL
                     AIQRTRGDVTDSLSERLPGFRSTPDAVYRLLRREAEEGYPFAEATLVSAHEHTASRFV
                     VDTRLVSGPLVRVARVVPRGNTITKSHVIARELRRTPGSAYSQKDVERWVRRLNRSGM
                     FDAVGEPALAWSDSASGTADLLISVREGRPNRFEGVVGYQPGEGNSRGEFTGLADVTL
                     GNLLGGFAMFALSVR"
     gene            complement(1237..2502)
                     /locus_tag="P5179_03510"
     CDS             complement(1237..2502)
                     /locus_tag="P5179_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94319.1"
                     /translation="MERKTVAVPLRGIVLPSLDRGAAERVAKWVGEWHSADYDPEEIS
                     STLANLPPEVPVDDLLEPFDIVAEEESAQALAELALALIHYVERGCLQAAEARDVFRM
                     ACLQLCNRGIPFEETAKGDDLAQWFHYIGVNEFGDTDDSGFSTDLTDLRLLSRYFLDP
                     GRLAEELFDKPHAEFPTNYHEGYIGIAGALGDWRAAPFLLRILSRCEDSPSVATTIAA
                     LGMLGRARKGWSAAYRDRVAQVLKGYAFSDAPEVATSAVLALEGLGGIHAEATLRELE
                     RVLPAGRDMLSAHVSHALIHLEKGDEALEKELARIASDPKEPQITRICAIERMAGSDE
                     SETICTLADLLDDPAVEPVRVDGYLTDDVVHVIREAAFMSIMDCRFRRLAEVLGEPVL
                     DRIEKFQTSYPPSWWIRARIGNREFEEDE"
     gene            complement(2533..3543)
                     /locus_tag="P5179_03515"
     CDS             complement(2533..3543)
                     /locus_tag="P5179_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRS94320.1"
                     /translation="MPAGRRSSFGFLWIAAVLAASGSSCGVLATQPYRSLAPVTPVQD
                     RDQAVQIIVAALTERGYSPALVNERVGTVSTDWRMTAGWMKIFFGYSGQDRVVVSLSD
                     STLILNGEYQMRKGTSLVGAMFHDRDDRRRDESDTDWFTTNPPASLRQEWEQLRLIIA
                     ERISQAAAARRATATGAASVVAAPPESAVAPPVLVRRQPAEQRVAVAVVDFKGVGVSR
                     DEVAILTDRLRAELVDTGIFMVIERERMEEILREQEFQISGACESDACVVEIGRLVGV
                     NRIVTGTIGKLGSTYTTTARLINVETGEIVARASDDCACEVEQLLGRMERIAGVLANG
                     AN"
     gene            complement(3663..5021)
                     /locus_tag="P5179_03520"
     CDS             complement(3663..5021)
                     /locus_tag="P5179_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS94321.1"
                     /translation="MRTIWRAVLREHIGPFFFAFAVVFFILVLDLLIDVMDSILGKGI
                     AVGTVLQLFVLNFAWIIALAVPMAVLVSVLMAFARLANDNEITALMAVGISPASLVVP
                     SVVAGGIVAAGLMWFNNAVLPESNFRARVLLNALREKKPAISLKSREGQFITDFPGYV
                     LRVDRVILESPDAGDDASGSRLEGILIYQFDQAGGTRPTVVTAKSGTIEIWDGGATVR
                     LVLNDGEMSQVDSKNPESDIRTQFQRQTIIIADRQRGNPEQLRRAEGFRGDRELSSAA
                     MLERARMYDRQVEAVDARIAALVADTGMAADYRRQQIASEERVRQGYRQMANQYRVEI
                     HKKFSIPVACIVFVLIGAPLGMMARGTGRSVAIVASLVLFLLYWASLIAGEQLADRGY
                     LPPWLAMWGANIIVGLWGIALLFSVTWNVRMASLMELVPRAIEARRSRKASSSAAKGF
                     VD"
     gene            5434..11721
                     /gene="sprA"
                     /locus_tag="P5179_03525"
     CDS             5434..11721
                     /gene="sprA"
                     /locus_tag="P5179_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS94322.1"
                     /translation="MPGKWCAASRFGALSVGLWLFSSFGTPSAQHLPPVAPAAFDSAG
                     TDTSSAVPDSGRVQLPRQRFSPYKPPQPGFFYTRKSQIDFSRGTVTTITSVGGVEIGE
                     RIVRPLDKAIAEDIAPRLAASLSQTRRQQGRPTTGGDTGPRIEMQYEIPNLPPVAKTI
                     IGEGPSTLHISGYGRITMSGRSQYRTGETVGSTYQQSKFPTLSMQQELSFKIDGTIGS
                     KIYVSVDQDTRRISELDNNVHIRYQGEEDEIVQEVEVGNTNLSLPGTQFVSGGVQHSG
                     LFGIKARAQFGDLDVTMIASQQKSSSQKRTFRGGAQASETKILDAEYIPRRFYFLDAS
                     YRIQYAAALEKLRNNPSMKDLDIGIVPGSGQRPWAREIVEVNLYRGVAATAGQVDVKD
                     GFGVPAMLTDSSGNDLTLSPYDPATLQAMAEGSGGRIRQGGWQLIPSTEYYLDPDLGY
                     VDFGTSLSDGAIIGVVIKFINQAGVIDSFPKCTDPSVLVAKMVKYAPSRPTDPTWTYE
                     WRHVYFVGARDLSPTDFRLEIVDAKVAGTPNTPANSAQTYLQLMQLDVASGTDLTGPA
                     DGLVDNNRVKLDLKTGLVYFPFLEPFQEAFVSLEGEGNPALYQETNLSGRDKYALVAT
                     YSKASTRIQLGFNIMENTEVVKLNGVRLTRNIDYTINYFTGELLFTQSVADQVSQPGA
                     DLSIDYEMNPIFKPDQESLVGLSGVYRLGERGNLGGIVMYNSERTSSTRVRVGEEPSR
                     MTVFATYANYEFRPSWITTAVDMLPFIESDEPSTLRLEGEIAQSLPNLNTRGTAYLDD
                     FEGAGNRTPVGIGRRGWALASPPVDLRLRPNELDRNRRGKFVWFNPYSRVKTIDVFTS
                     LSENELTQSERFMDVLNLWFRPKGTTDSERQASWGGIMRSFGTTGVDLQKTQFLEIWV
                     RAGANPFDPDRIGGVARPTGTPVLHIDLGDMSENSHLSTRTGIFRLTDFNTEDYIINT
                     KYVDQRWESYRNNGILDFSGNDRGEDVGLDGCPDAYEDGTGGCLDTPNPDYPAVTDDP
                     NGDNWSFRHTGSPYDFEHINGTQDNRQDSEIQPYPDNEDLNGNGSLDRTNSYLTYTIE
                     LDENSPYAVEETEQARSGFRLYRIPLKELGMGTVDSVGGTAPAFNRITTARMWVDGVR
                     DSALWVTIASMDFVGNDWEEVKQAGEQFATTTVNTQDNKAYTPPPGVERELDALTGAL
                     RREQSLVMQIMEIAPGQTYRAQRDLYTAVNLTDYRRLRMYVHGPETGINPTWDHLAAF
                     LRLGLDSTSYYELRIPRLYPGWDERNYVDVFIDSLTNLKLTDTGQFFSGRDTISTDGR
                     LRVVAAQRGASVTLPSLSSIRVLQIGIVNLSDHTIRAPSTNQPIQVWFDDLRLEGVRN
                     IAGRAYRAGLKMQMADLVNLQVSTNRQQIGFGSIQNKQGSGTETRSYTVAVDRFRLDK
                     LLPPAWRVSVPVNLNYSVNTNVPRLKRGSDIELIDQRDKDVERARTATINASTSFVKS
                     SQSRNPAIGLTLDRVGAGVTYSLSHGLTPGLTSRDSSHSMSYTGRFNYDLTPRRPHQL
                     RILGWVPDRLPGKVEETEFAYLPTSLRFDATTTFRADSSWSKQKVLGRDTTLARGQRV
                     FTISEAYRIGFQPFRSLTTAYDMSLNRDLADALEEDTGPGAVMQSLAKNIFRNREIRR
                     SQNTSINYSPPWPWWLGQSYTYTNQYNDNSDPRTTSGSTSGARQYEVTNSRGYGISSM
                     TFKVRDILTRLSGETGGTGGGLRRGGMAPAAPRRPVSTDTTGGFSLLRPLRATAGFGG
                     NHLENVVGRITFNERFMGTQIPDSLRPDLKYQILGIGRHPVLPGVSYTIRPQNSISQD
                     LGWDVSSGLQLPLRMRLQAKYAFRTTKTMGARDTTRSRDITFPDVGYSWDGLESLPLL
                     RIVTTRSEVQSSFTRRINTSWTRTGGLPERLDRRSTSYVLSPLFSWSILWKNSIRSTI
                     RSTWDQTLDERYSAAGISSTQTTNWGVTLNTTYELQTSRGISKPWGGKWQLAGNINLS
                     LDGSYSGSYAVGLGANPSTGGDLVQGHTRTWSIKPQAQYQFSRTFTGQAQIEVGSMTD
                     LRNHYTMKTRAVMITGELRFN"
     gene            11771..13231
                     /locus_tag="P5179_03530"
     CDS             11771..13231
                     /locus_tag="P5179_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94323.1"
                     /translation="MKRVKIGWATRDVTTTEPVGIPGQFHLRISEGVLDPLTVTALVV
                     DGGDDTAIFLSADLVVIRSFLLDLVREKVARLNPEIPVLKILMNATHTHTGGDHYTSG
                     SDDFPCDLPRYSGDEYRELLATGCAEAVAEAWATRAPGGVAWGYGHAVVSYSRRVVYF
                     DDVSQRPGATRDSAAGVNGHARMYGNTNDPMFSHYEAGADHFVNLLFTFDAENRLTGA
                     IVNVPCPSQVSEHLWQLSADYWHDVRLAIRKQFGNIFILPQCAAAGDLSPHILHYKKA
                     LERRLALKGMSERQDIAERITTAFAEVYSWARNDVRTEVPVRHAVETVELSRRRITED
                     EFEEDSRMLEEFNKKQFATEGTPKERLIHDSFLSALRKRAQGVIDRYRTQDANPKLPM
                     ELHVIRIGGVAFASNRFELYMDYMHRIQARSPFEQTFVVQLAGVPGPDGGTYLPTERA
                     VWGKGYGASRYCNQVGPEGGQELVEHTLRLLNGVKD"
     gene            complement(13552..>13738)
                     /locus_tag="P5179_03535"
     CDS             complement(13552..>13738)
                     /locus_tag="P5179_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94324.1"
                     /translation="KHMAGRNACPTACGCARSPSGANVRAADAALYRGARTGEILRFS
                     SALRTLLRMAVLRVYEE"
BASE COUNT         2809 a         4370 c         4100 g         2449 t
ORIGIN      
        1 gggtactgag tcgctgcgga cgatttcgcg gccgatgctt gccgccacca tcacgtcggg
       61 gacgagttct ccgccaaccg ccagttcaag acgctcgagc gcgtacgcgg tgcgctgctc
      121 aacggcaagc ttcccttccg catccacagg aaagccggcg acccacggct cccggtacga
      181 aaggtccagc gtggtctgtt ctgcctgagg acgattccag cgaaccgcaa gccgacgtcc
      241 cgtacnnnnn nnnnngcgca cgctcagggc aaacatggca aagccgccca gcaggttgcc
      301 cagcgtcaca tccgcaagac ccgtgaactc tccgcgggag ttgccctcgc ccggctggta
      361 accgacgaca ccctcaaacc tgttcgggcg gccttcccga acggagatga gaagatcagc
      421 ggttccggac gcggagtcgc tccatgccag ggcaggctcc cctacggcat cgaacatccc
      481 cgaacgattc aaccgccgta cccagcgctc aacgtctttc tgggaatacg cgcttcccgg
      541 cgtccggcgc agttcgcgcg caatgacgtg acttttcgtg attgtgttgc cccggggaac
      601 cacgcgtgcg acacgcacga gagggccgga caccaggcgc gtatccacca cgaatcgcga
      661 agcggtgtgc tcgtgcgctg atacaagcgt cgcttcggca aacgggtatc cctcctctgc
      721 ttcccgtcga aggagccggt acactgcgtc gggtgtggat cgaaaacccg gcaggcgctc
      781 cgaaagggaa tccgtcacgt ccccccgcgt gcgctggatg gcaagggtga cgctggcgat
      841 atcgccctcg tgaaccagta tttcgcgcac ggcgtccccg tttcgggaga cgtataacgt
      901 gtcctgcaga atggcggacc agaagcctgc atctgagtat tccttcccga tgcggcgcaa
      961 cccgtcaggg ccggacgccc acagacggat gcgatccgcc ggaaacgccc tcgcgccgcg
     1021 gacaaccacg cgaaccgagt cagtctggac ggcatcggcg gggcagactt tggcgcagca
     1081 gaacacggcg cacgcgccgg tcgcaagggc agcgattctg cgtatcatgg gcagcggttc
     1141 cggggtctgc cggatgtggg aaacgctccg gaacccgcag gagcacgccg ggccgaatgg
     1201 agggaggggg acatcgggcg aaccccaacc ggaggactac tcgtcttcct cgaactcccg
     1261 gttgccgatt cgggctcgaa tccaccacga cggcggatag gaggtctgga acttctctat
     1321 acgatcgaga accggttctc cgagcacttc cgcaagacga cggaaccggc aatccatgat
     1381 ggacatgaag gccgcttccc gaatcacatg cacgacgtcg tccgtgagat acccgtccac
     1441 gcggaccggt tccactgcgg ggtcgtccag caggtcagcg agggtacaga tggtttctga
     1501 ttcgtcgctt cccgccatgc gttcgatggc gcatatgcgg gtgatctgtg gttctttcgg
     1561 gtcggaagcg atgcgcgcaa gttctttttc cagcgcctca tccccttttt caaggtggat
     1621 cagcgcgtgg ctgacgtgcg cggagagcat gtcgcgcccg gcgggaagca cccgttcgag
     1681 ttcgcgcagg gttgcttctg cgtgaatacc gccaaggccc tccagagcca gcaccgcgga
     1741 agttgccacc tcaggagcgt cggaaaaggc gtaccctttg agcacctggg cgactctatc
     1801 gcgatacgcc gccgaccacc cctttcgggc gcggccgagc atccccaggg cagcaatcgt
     1861 ggtggcgacg ctgggggagt cttcacaacg gctcaggatg cgaaggagaa agggcgcagc
     1921 cctccagtct cccagcgcgc ctgcgatacc gatgtaacct tcatgataat ttgtggggaa
     1981 ttccgcatgg ggtttgtcaa aaagttcttc cgccagccgc ccgggatcga ggaaatagcg
     2041 cgacagcagt cgcaggtcag tcagatcggt ggagaacccc gaatcgtcgg tatcgccgaa
     2101 ctcattgacg ccgatataat gaaaccactg cgcgaggtcg tcccctttgg cggtttcttc
     2161 gaacggaatg ccgcgattgc acagttgcag gcacgccata cggaacacat cacgcgcctc
     2221 agccgcctgg aggcacccgc gttcaacgta atggatcagg gcgagcgcca gttccgccag
     2281 cgcctgcgcg ctttcctcct ccgcaacaat gtcgaacggc tccagaaggt cgtccacggg
     2341 aacctcggga ggcaggttcg cgagggtgga cgaaatctcc tcgggatcat agtcggcact
     2401 gtgccattcg ccgacccatt tcgccacacg ttcggcagcg ccccggtcaa gagacggcag
     2461 gacgatcccg cgaaggggaa ccgctacggt ttttcgctcc atgtgcgact ccggcagggg
     2521 aaatggggtg gatcagtttg cgccattggc aagaacgcca gcgatccgtt ccatccgtcc
     2581 gagcagttgc tccacttcgc acgcgcaatc atccgacgcc cgggcgacga tttccccggt
     2641 ttccacgttg atgaggcgtg ccgtggtggt gtacgtactt ccgagtttgc cgatcgttcc
     2701 cgtaacgata cggttgacac cgacgaggcg accgatttcc accacgcagg cgtcagattc
     2761 acacgcaccg gaaatctgga actcctgttc gcgcagaatc tcttccatgc gttcacgttc
     2821 tatcaccata aatataccag tgtccacgag ctcagcccgc aaacggtcgg taagaatggc
     2881 cacttcgtcc cttgataccc ctaccccttt gaaatccacc acggccaccg ctacccgttg
     2941 ctctgccggc tgacgacgaa ccaacacggg cggcgccaca gccgattcgg ggggcgccgc
     3001 aaccacggaa gccgcaccgg tggccgtcgc gcggcgcgct gcagccgcct gtgagatccg
     3061 ctccgcaatg atgagccgca gttgttccca ctcctgacgc agtgaggcgg gtgggttcgt
     3121 ggtgaaccag tcagtgtccg actcatcccg gcgcctgtcg tccctgtcat ggaacatggc
     3181 gcccacaagc gaggtgcctt ttcgcatctg atactcgccg ttgagaatca gcgtgctgtc
     3241 ggaaagcgac acgaccacac gatcctgccc cgaatagccg aagaagatct tcatccaacc
     3301 tgccgtcatg cgccaatcag tggaaacagt acccacgcgt tcgttcacga gggcggggct
     3361 gtatcctcgc tcagtcaggg cggcgacgat aatctgaacg gcctggtccc ggtcttgaac
     3421 aggcgtgacg ggagccagcg aacggtacgg ctgggtggcc agaacgccgc agctcgatcc
     3481 ggacgcggca agcaccgcgg cgatccagag gaatccgaag ctggaacggc gacctgcagg
     3541 caaggctgga tagactcgtt cccaatggtg aagacggact gtccggcacg gactgcccgg
     3601 tacggactgt gaggcgtgtc acattgctga cgtattaagg gtaccccgcg gggggcgatt
     3661 tgtcaatcaa cgaagccctt cgctgccgac gacgatgcct ttcgcgatct ccgcgcctca
     3721 atcgcacgcg gcacaagctc catgagactc gccatccgga cgttccacgt tacgctgaac
     3781 aacagggcga tcccccacag cccgacgatg atgttggccc cccacatcgc cagccagggc
     3841 gggaggtatc ctctgtcggc aagttgttca ccggcgatga gagacgccca gtagagaagg
     3901 aacagcacga gactggcgac aattgccacc gagcgaccgg tcccgcgtgc catcatgccg
     3961 agcggagccc cgatgagaac gaatacgatg caggcgacag gaatcgagaa cttcttgtgg
     4021 atttccacgc ggtactgatt tgccatctgc cggtagccct ggcgcaccct ttcttctgac
     4081 gcaatctgct gcctgcggta atccgccgcc atccccgtgt cggcaaccag cgcggcgatc
     4141 cgtgcgtcga ccgcttcgac ctggcgatcg tacatgcgtg cacgctccaa catggccgcg
     4201 gaggagagct cgcgatcacc gcggaacccc tccgcccgcc gaagctgctc agggttcccc
     4261 ctctgtcggt cggcaataat gattgtctgc cgttgaaact gcgtgcgtat gtcactttcc
     4321 gggtttttcg agtcaacctg actcatttcc ccgtcgttca ggacgaggcg caccgtcgcg
     4381 ccaccgtccc agatttcaat cgttccgctc ttcgcggtca cgacggtcgg ccgggtcccg
     4441 cccgcctggt caaattgata gatcagaatg ccttccaggc gggaccccga ggcgtcgtcc
     4501 cccgcatccg gtgattccag tatcacccta tctacgcgca ggacgtaccc ggggaaatca
     4561 gtgatgaact gcccttcccg gcttttgagg ctgatcgccg gcttcttctc tctcaaggca
     4621 ttcaggagaa cccgtgcccg gaaattgctc tccggaagaa ccgcattgtt gaaccacatg
     4681 agaccggcgg ctacgatacc gccggccacc acggacggga ccacaagtga ggccgggctg
     4741 attccgacgg ccatcaacgc cgtgatctca ttgtcgttgg ccaaccgcgc aaacgccatg
     4801 agcaccgaaa caagaaccgc catgggcacc gcgagcgcga tgatccacgc gaagttgaga
     4861 acaaaaagct gcagaaccgt tccgacggcg atccccttgc ccagaatgct gtccatgacg
     4921 tcgatcagca aatcgaggac aaggatgaag aacaccaccg cgaacgcgaa aaagaacgga
     4981 ccaatgtgct cccgcaggac ggctctccag atcgttcgca tatgcgcgca tcctgaattc
     5041 gtaccggcca ccagatcgcc ggggttgtaa agccggtttc cgcgtgggta tactctacgt
     5101 tcgctccagc ccggtcgggg gcgacgggtt ccccggcgtc accgggggtg tcttcttctc
     5161 gcaaaggccg tgaatatcgc ccactgcgtt cgttcctcgt gcgaaacacg cgacacgtcc
     5221 ctctggtgaa cgtgcggcgc cactccgtgg tatggcggaa ggtaccgatg ccacgtcgtg
     5281 cacgcaaact ccggggccgt gtgtgccgtt tccgtgcctc cgggtggccc gccatccggt
     5341 tgcgcgggtc ccggggaaac ccatacgtcg gtagtctcgt ggtttcggtt ggcagccccg
     5401 cccttctggt ggtttgagag ggagcttcct cacgtgccgg gaaagtggtg tgccgcgtct
     5461 cgatttgggg ccctttccgt gggattgtgg ctcttttcga gtttcggcac gccctccgcg
     5521 caacacctgc cgccagtcgc ccccgcggcc ttcgattcgg cgggtacgga tacctcgtcg
     5581 gctgttcccg actccggccg cgtgcagctc ccgcggcaac gtttctcgcc gtacaagcca
     5641 ccccagccgg ggttcttcta cacccgcaaa tcccagattg atttctcccg cggcacggta
     5701 accacaatca cctccgtggg tggcgtggaa atcggagaac ggatcgtcag gccgctggac
     5761 aaggcaatcg cagaggacat tgctccgcgg ttggcggcca gcctcagcca gacacgccgc
     5821 cagcaggggc gccccaccac cggcggcgat accggcccgc gcatcgagat gcagtatgag
     5881 attcctaacc tgccgccggt tgcgaaaaca atcattggcg aaggccccag cacactccac
     5941 atatcggggt acggccgaat cacgatgagc ggcaggagcc agtaccgcac cggggaaacc
     6001 gtaggttcca cctaccagca gagcaagttc cccacgcttt ccatgcaaca ggaactcagt
     6061 ttcaaaattg acgggaccat tggaagcaaa atctacgtca gcgtggatca ggatacgcgc
     6121 cggatcagcg agctggacaa caacgtccat atccgctacc agggggaaga ggacgagatt
     6181 gtccaggagg ttgaggtcgg caacactaac ctctccctgc ccggaacgca gtttgtcagc
     6241 ggcggcgttc agcacagcgg tctgttcggc atcaaagcga gggctcagtt cggcgatctc
     6301 gacgtgacaa tgatcgccag ccagcaaaag agctcatcac agaaacgcac cttccgcgga
     6361 ggcgcacagg cttccgaaac aaagattctg gacgcggaat acatccctcg ccggttctac
     6421 ttccttgatg cgagctaccg aattcagtac gctgccgcgc tggaaaagct tcgcaacaac
     6481 ccgtcgatga aagacctcga catcggaatc gttcccggct cagggcaacg gccgtgggcg
     6541 cgggagatcg tcgaagtgaa cctgtaccgg ggcgttgcgg cgacggccgg acaggtggac
     6601 gtcaaagacg gattcggcgt tccggccatg ctcacggatt cttccggcaa cgatctgacg
     6661 ctttcaccct acgaccccgc aaccctgcag gcgatggcgg aaggaagcgg gggccgcatt
     6721 cggcagggcg ggtggcagct catcccgagc accgaatact acctcgaccc cgacctcggg
     6781 tacgtggatt tcggaacgag cctctccgac ggggcgatca tcggcgtggt gatcaaattc
     6841 atcaaccagg cgggggtgat tgattcgttc cccaaatgca ccgacccgag cgtgctggtt
     6901 gccaagatgg tgaaatacgc gccgagccgg cccaccgacc cgacatggac gtacgaatgg
     6961 cggcacgtct atttcgtcgg tgcgcgtgac ttgagtccga cagatttccg gttggaaatc
     7021 gttgacgcaa aagtggcagg cacgcccaac actcccgcca attcggcgca aacgtatctc
     7081 caactgatgc aactggacgt ggcgtcggga accgatttga caggacccgc ggacggtctg
     7141 gtggacaaca accgggtgaa actcgacctg aagaccggtc ttgtgtactt ccctttcctg
     7201 gagccgttcc aggaggcgtt cgtttctctt gaaggtgaag ggaatcctgc gctttatcag
     7261 gaaaccaacc tgtcgggtcg tgacaaatac gcgctggttg cgacgtacag caaggcctcc
     7321 acacggattc agctcggatt caacatcatg gaaaacacgg aggtggtgaa actgaacggc
     7381 gtgcgcctca cgcggaacat agattacacc atcaactact tcaccggaga actcctgttc
     7441 acgcaatccg tggcggatca ggtaagccag cccggcgcgg acctgagcat cgactacgag
     7501 atgaatccga ttttcaaacc cgaccaggag tcccttgtag gtctgagcgg agtgtaccgg
     7561 cttggcgaac ggggcaacct cggcggaatc gtgatgtaca acagtgagcg aacctcgtcc
     7621 acgcgcgtgc gggttggtga ggaaccgagc cgcatgacgg tgtttgccac gtacgcgaac
     7681 tacgaattca ggccgtcgtg gatcacaacg gcggtggaca tgctcccgtt catcgaaagc
     7741 gacgagcctt ccaccctgcg cctcgaaggc gaaatcgcgc agagcctccc gaacctcaac
     7801 acgcgcggta cggcttatct ggacgacttc gaaggggccg gcaaccggac tcccgtcggt
     7861 atcgggcgca gggggtgggc gcttgccagc ccgccggtgg atctacggct gcgccccaat
     7921 gaacttgacc gcaaccgccg cggcaagttc gtatggttca acccgtacag ccgggtcaag
     7981 acgatagacg tgttcaccag cctctccgaa aacgagctca cccagtcgga acgcttcatg
     8041 gatgtcctca atctctggtt ccggcccaaa gggacgacgg attccgagcg ccaggcaagc
     8101 tggggaggaa ttatgcgttc cttcggaaca acgggggtgg acctgcagaa aacgcaattc
     8161 ctggagatat gggtccgcgc aggtgcaaat ccgtttgatc ctgaccgtat cggaggggtt
     8221 gcgcggccaa ccggaactcc cgtactgcac atcgacctcg gggatatgtc agagaattcc
     8281 cacctgtcta ccagaaccgg gatcttccgg ctgacggact tcaacacgga agactacata
     8341 atcaacacga agtatgttga ccaacggtgg gaaagttacc ggaacaacgg gatacttgac
     8401 ttcagcggca acgaccgggg cgaggacgtt ggccttgatg gctgcccgga tgcgtatgaa
     8461 gacggtaccg gcggctgtct ggatacaccc aacccggatt acccggccgt gaccgatgac
     8521 ccgaacggag acaactggtc gttccggcac acgggctccc cgtacgattt tgagcacatc
     8581 aacggaacac aggacaaccg gcaggattcc gaaatccagc cgtatcccga taacgaggac
     8641 ctgaacggaa atggctcgct tgacaggacg aacagctacc tcacgtatac catcgagctc
     8701 gacgaaaaca gcccgtacgc ggttgaagag acggaacagg cgcgctccgg cttccggttg
     8761 taccggattc ccttgaagga actcggcatg ggcaccgtgg attcagtcgg aggaacggcg
     8821 cccgcgttca accgaatcac cacggcgcgc atgtgggtag atggcgttcg cgattcagcg
     8881 ctctgggtta ccatcgcctc gatggacttc gtcgggaacg actgggaaga ggtgaaacag
     8941 gcgggcgaac aattcgcaac taccacggtg aacacgcagg acaacaaggc atacaccccg
     9001 ccgcccggcg tggaacgaga actggacgcg ctaaccggcg cgctgcggcg cgagcaatcg
     9061 ctcgtgatgc agatcatgga aatcgccccg gggcagacct accgcgcaca acgcgacctc
     9121 tacactgccg tgaatcttac cgattaccgc agactgcgca tgtacgtgca cggccccgaa
     9181 acaggcatca accccacgtg ggaccatctg gccgcgttcc tgaggctcgg gctggactcg
     9241 accagctact atgagctgcg gatcccgcgc ctctaccccg gctgggatga gcggaattac
     9301 gttgacgtgt tcatagactc gctgaccaat ctcaaactga cagataccgg gcagttcttt
     9361 tccggacgcg atacgatctc caccgatgga aggcttcggg ttgtggcggc acagcgcggc
     9421 gccagcgtaa ccttgccctc gctttcctcg attcgcgtgc tgcagatcgg cattgtcaac
     9481 ctgagcgacc ataccatacg cgcaccgtcc acaaatcaac ccatccaggt ctggttcgat
     9541 gatcttcggc tcgagggcgt gcgcaatatc gccggccggg cgtaccgtgc cggtttgaaa
     9601 atgcagatgg ctgacctggt gaatttgcag gtttcgacga accggcagca gatcggcttt
     9661 ggaagcatcc agaacaagca gggcagcggt acggaaacac ggtcttacac agttgcggtg
     9721 gatcggtttc ggctcgacaa actacttccc cccgcctggc gggtgtccgt tccggtcaac
     9781 ctgaattact ccgtcaacac gaatgtccct cgtctcaaac ggggttcgga catcgaactg
     9841 attgatcagc gcgataagga cgtggagcgc gcgcggacgg cgaccataaa tgcgtccacg
     9901 tccttcgtga agagctccca atcgcgcaat ccggccatcg ggctcactct cgatcgtgtg
     9961 ggggcgggtg tcacgtacag cctttcgcac gggctcacgc cgggtcttac ttcccgggat
    10021 agctctcact cgatgagcta cacggggagg ttcaattacg acctgacgcc gcggcgacct
    10081 catcagctcc gcatcctggg atgggtaccg gaccgccttc ccggcaaagt ggaggagacg
    10141 gaattcgcct acctgcccac cagcctccgg ttcgacgcca ccacgacgtt ccgcgctgac
    10201 tcatcatggt cgaagcagaa ggttctcgga agggatacga cgctggcgcg ggggcaacgg
    10261 gtgttcacga tttcggaagc ataccgtatc gggttccagc ccttccgctc gctgacaacg
    10321 gcgtacgaca tgtcgctgaa ccgcgatctc gccgatgcgc tggaggagga taccggtccc
    10381 ggagcagtaa tgcagtcgct ggcgaaaaac attttccgaa accgggaaat acgccggtcc
    10441 cagaacacga gcatcaacta ctcgccgccg tggccgtggt ggttgggcca gtcctatacc
    10501 tacaccaacc agtacaacga caacagcgac ccgcgcacca cctccggaag cacctccggc
    10561 gcacggcagt atgaggtcac caactcgcgg gggtacggca tatcgagcat gacgttcaaa
    10621 gtgcgcgata tcctcacccg tctttccggt gaaaccggag gaacgggcgg cggtttgcgg
    10681 cgcggcggaa tggcgccggc cgctccgcgg cggccggttt cgacggacac cacgggaggc
    10741 ttctctctgc tccgccccct gcgggcaacg gccggtttcg ggggcaatca tctggaaaac
    10801 gtggtcggcc gcatcacgtt caacgagcga ttcatgggca cccagattcc cgattcgctc
    10861 cgtcccgatc tcaaatacca gatactggga atcggcaggc acccggtgtt gcccggtgtg
    10921 tcgtacacca tccgcccgca gaactccatc agtcaggatc tgggctggga tgtatcaagt
    10981 ggtctgcagt tgccgttgag gatgcggctg caggcgaagt acgcgttccg cacaaccaaa
    11041 accatgggtg cgcgcgatac cacgcgatcc cgcgatatca ccttcccgga cgtcgggtat
    11101 tcatgggacg gcctcgagtc gttgcctctt ctccgcatcg tcaccacgcg cagcgaggtc
    11161 cagtcctcgt tcacgcgccg gatcaacaca agctggacga gaacaggcgg gctccccgag
    11221 cgcctcgacc gccgttccac ctcgtatgtg ctgagcccgc tcttctcgtg gagcatcctc
    11281 tggaagaaca gcattcggtc cacgatcagg agcacatggg atcagacgct cgacgagcgg
    11341 tattccgcgg cgggaatctc ttcgacccag acaaccaact ggggcgttac gctcaacacg
    11401 acgtacgagc ttcaaacgtc gcgcggaatc tcaaaaccgt ggggcggcaa gtggcaactt
    11461 gcgggcaaca tcaacctgtc gcttgacgga agctactccg gcagctacgc cgtcgggctg
    11521 ggtgccaacc cctccacggg tggcgacctt gtgcaggggc atacacgcac gtggagcatc
    11581 aaaccacagg cacagtacca gttcagccgg acctttaccg ggcaggcgca gatagaagtc
    11641 ggctccatga cggacctgcg caaccactac acgatgaaaa cccgtgccgt catgattacc
    11701 ggagaactcc gattcaactg agcgggcgat caacaccgca gacggccgcg ctctgacaag
    11761 gggacacagg atgaaacgcg tgaaaatcgg ctgggcaacg cgcgacgtga ctaccaccga
    11821 accggtgggc attccggggc aatttcacct gcgcatctcg gagggcgtcc tcgacccgct
    11881 caccgtcacc gcactggtgg ttgatggtgg ggacgacacc gccattttcc tttcggccga
    11941 tctcgtggta atacgttcat ttctgctcga cctcgtacgg gaaaaagtag cgcgcctcaa
    12001 ccccgaaatc ccggtgctca aaatcctgat gaacgccacc catacgcaca ccggcggtga
    12061 ccattacacc agcgggtccg atgacttccc gtgcgacctg ccccgctatt ccggcgacga
    12121 ataccgcgaa ctcctcgcga ccggttgcgc ggaggcagtg gccgaggcat gggcgacgcg
    12181 cgcccccggt ggggtagcgt gggggtacgg ccacgcggtc gtctcttaca gccgccgggt
    12241 ggtctatttc gacgacgttt cacagcgccc cggcgcgacc agggattccg ccgccggtgt
    12301 gaacggccat gcaaggatgt acggcaacac caacgatccg atgttcagcc attacgaggc
    12361 cggcgcggac cacttcgtca atctgctctt cacgttcgac gcggaaaatc ggctcaccgg
    12421 tgccatcgtc aacgtgccgt gcccgtcgca ggtgtcggaa cacctctggc agctttccgc
    12481 cgattactgg cacgacgtcc gcctcgccat ccgcaagcag ttcggcaata tcttcatcct
    12541 tccccagtgc gccgctgcgg gggatctttc cccgcacatc ctccattaca agaaggcgct
    12601 tgaacgtcgt ctcgcgctga agggcatgtc cgaacggcag gacattgccg aacggatcac
    12661 caccgccttc gccgaggtct actcgtgggc gcggaacgat gtccgtaccg aggttccggt
    12721 gcgccacgcc gtcgaaaccg tggagctttc gcggcggagg atcacggagg atgagttcga
    12781 agaagacagc cggatgctcg aggaattcaa caagaagcag ttcgccacgg aaggcacccc
    12841 gaaggagcgc ctgatccacg attctttcct gtcagctctg cgcaaacgcg cgcagggagt
    12901 cattgaccgt taccgcacgc aggacgccaa cccgaaactg cccatggagt tgcacgtcat
    12961 ccgaatcggt ggcgtggcgt ttgcctcgaa ccggttcgag ctctacatgg actacatgca
    13021 ccggattcag gcacgcagcc cgttcgagca gacgttcgtg gtgcagcttg ccggcgtgcc
    13081 ggggcccgac gggggcacct acctgccgac ggagcgcgcg gtgtggggca agggatatgg
    13141 cgcctcgcgc tactgcaatc aggtcggccc cgagggcggg caggagcttg tggagcacac
    13201 cctgcggctg ctgaacgggg tgaaggactg attgcccgcg gatcgtcgcg cgggaccaac
    13261 gtcgtcgagt cgggtgcccg gcgcttcgac aggctcccgc aggcgggagc acgctcagtg
    13321 accggggcac cgattggtgg ggcttggtcc ccacgggaac aggacattcc tgtctgtccc
    13381 cgctttcctg cgcggccttt ttcgtgccac tacaaggtcg tcgagtggcg cgccccgtcg
    13441 gcgcgctgta tcgagacgac cgattcccgc cccaccgtag cacgggcgtc ccgcccgtgc
    13501 gcgcaaattg ccccattcag aaactgcacg ggcaggatgc acgtgctacg gttattcttc
    13561 gtatacgcgc aacaccgcca ttctgagcag cgtccgcagc gcggacgaaa agcggagaat
    13621 ctcccccgtc cgggctcctc gatacaacgc tgcgtccgcg gcgcggacgt tcgcgccact
    13681 cggagaccgg gcacacccgc acgcggtggg gcaggcattc ctgcctgcca tatgcttc
//
LOCUS       DAOTNG010000045        13691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4387_length_13691_cov_5.234746, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000045 DAOTNG010000000
VERSION     DAOTNG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_13691_cov_5.234746"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="P5179_03540"
     CDS             <1..1173
                     /locus_tag="P5179_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94325.1"
                     /translation="RQDGGSWGKIAEQQKAGEREPCPIALGPGGTLLLSVSTAVSPSH
                     DNHDGTTGFHCLPHVLSFRIAGGILPEPKVIIPQWNAEYPFNEHSYRGFSSDASGNVF
                     LTHQASDGSEYGQAWCYRTSEGDWVAAGFLRFPMRGCYPAAAVNGKAVAVLAISDEIE
                     PNEEWRSFKKERTARDWDYDFRQLFFTWTPDVTAVPFSVPLTIASVDETCGVITHTDL
                     LVTPDGDVHVLYIERNVYHDFVRERFFPDLPLSVSLKYVRLRRGCVVERRILCKSCEK
                     FDNTRAETAVKMGAPVPTWAVFHATGAGRVFLVWNQTDNVASQNGMFLRQIIPQWDTT
                     AISLPVRTPLTQFFAASTRAGCLPDNRLDLYGTAPGEQVIRTIHCVVETDQAEVTR"
     gene            1170..2411
                     /locus_tag="P5179_03545"
     CDS             1170..2411
                     /locus_tag="P5179_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94326.1"
                     /translation="MTTPRERVARAVNFQPTDRVPIDLGGMKATGIAVKAYNAVKKQI
                     GLSSPTRIWDPKFMIAHVEEEIRQRFHLDVVPLDVSSVSDEMRPECEWVPMALYEGAE
                     GLIPPGTMIGEDDRGRWVLMGADGRPTALRMPREGYYFDDISFDRPGMEIRPSEFQPE
                     ATFSDEQLRAVEARADFLFSQTDYAVLGWGGGVCFLGLSLITDRSSNVTMNCPTQWMT
                     MLITEKELCHEMMGRSVDAAISRLSLLHEAVGNRCVAWGIAADDAGTQRGEFIRPDLW
                     AEMIKPHYARLCSWVHSHTEWKTFLHSCGSVYHLIPHMIEAGVDILNPVQTSAANMEP
                     ERLTREFGGKLVFWGGGCDTQHVLGNGSPEEVRTHVRERLELFKTGGGYVFTQVHNIQ
                     PNVPPENIVAMLDAAYEYGVF"
     gene            complement(2415..>3411)
                     /locus_tag="P5179_03550"
     CDS             complement(2415..>3411)
                     /locus_tag="P5179_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRS94327.1"
                     /translation="AEIYGTIERENMEVIRLDKAPRIAVYTPPNKQPWDDAVTLALTY
                     AEIEYTTLWDREVLAGELRNYDWLHLHHEDFTGQFGRFYISYQTAPWFLQQKAELERT
                     ARELGFAKVSELKKAVARAIREYVANGGFLFAMCSATDTIDIALAAEGVDIVPAICDG
                     DGVTPDYNNRLDFSKTMAFENFRLEENPWVYEFSDIDTYDDRAANPRGPEADFFTLFD
                     FSAKLSPVEAMLTQNHVDHVRGFLGQTVGFHRRFLKPSVIVLGESEGEDEVKYVHGQV
                     GTGTFTFLGGHDPEDYQHLVGDPPTDLNFHRNSPGYRLILNNVLFPAARKKEHKT"
     assembly_gap    3412..3511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3543..5390)
                     /locus_tag="P5179_03555"
     CDS             complement(3543..5390)
                     /locus_tag="P5179_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94328.1"
                     /translation="MDVTFLLRAVSLRTSVVTRIWSECALGANAILLILLHTAAPAAV
                     TPPREIIGWFVQAQALEHANRPLDAAAYYRRVWQAAPTRSDAVLRGTRLLTENGRFDE
                     AVAWLGEATANTPEDAVLWVEFGDVFDRTGYTVQADSVWRIGSRTSSDPIAFHIGVAD
                     RLVSRGQLRRAQTWLRACNAQGDGQRVVNRRLLDIALALGDAHATAEAASGFVAGDVD
                     RVREAREIIQGASLTPAMMQTVTRAAIALSDSTPRDAARALFAAEMAVLGAQQEDAVR
                     LFLRSAKETRNPGTTLATMIDELEARGQTPVATFLMERLASDYPESPSAQRYAYTAAG
                     ILDEMGFQDRARNLLSWIISVPFSPYHDDARIALGRIFLALDSVEQAEQQFDWAATRG
                     RTPAHRRQGILGRGECALRAGHLEEAQSFWQMLADSAMKEPESLKAHLRLAQAAMFRG
                     DAPGLRARCELMVGNAAPGDETNDCLRLAEILEQAADDSVSWVRYGHLEFLFATGHRD
                     SVLLLAPRLFGTPLEGWARLLVAEVQIREGHYSLAATTLEAAASRLDSSSAGEEALWR
                     YAELQRTRLQDSGEETRALETLLMTYPGSVYGNQARRRLRELRAQPGAL"
     gene            complement(5471..6517)
                     /locus_tag="P5179_03560"
     CDS             complement(5471..6517)
                     /locus_tag="P5179_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRS94329.1"
                     /translation="MSLLTQSTFRRHVLEVCVALGAALNLSCSEQRTKLVVYSPHGRE
                     MLSEYERMYEEANPHVDVTWYPMGAEVGIERLRGEKNNPQADLWWGGPSALFEQGARE
                     GLLAPYHPSWREAAPPGSFSDEGLWYSTFQTVKVIGFNSDRLTEQTAPQDWDDLLEKR
                     WEGKVIIRSPMESGTMKSVFAAMVYRFFAEDGTPDRGYDWLRRLDANTKAYAASPATL
                     MLMIGRQEGLVTLWDLTDILLQKREQNVPMGFVIPRSGALVLNEGIAIVNGAPHRAEA
                     ERFYEFVTTKKALLHQAERFYRIPARRDISDQEKPGWLRSIQITELPVDWNVINAHRE
                     AWMQYWDQHIKGHH"
     gene            6715..7401
                     /locus_tag="P5179_03565"
     CDS             6715..7401
                     /locus_tag="P5179_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRS94330.1"
                     /translation="MITVWDWVILALFAALVAQDTTAGPQLLFSEPLVAGTVAGLVLG
                     DLRLGLLLGTAIQLIWCGAVPAGTAVFLDINVGTVCAVAVAHSAGSAPIAAAASMLWL
                     IPVGLLGCALTEVGMRFCGKLVASIQPECVSAGALVLRHGAGWLFAGARGSVTFLAGA
                     GAGVAVAPVLVRFLGAAVDPGVLWAGLFGAGAGSAIAATWRFSKGKAAFLGAAGVFGA
                     WAGGLLPVSW"
     gene            7414..8220
                     /locus_tag="P5179_03570"
     CDS             7414..8220
                     /locus_tag="P5179_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRS94331.1"
                     /translation="MTSLTRVSSSETAPVVPLWVRMEVVLRAFWIQASLNYQSMQGLG
                     RLVCLLPVMRWLSLPGKEFASFVRRHSGLFNSNPFVATLGLGALARLEFDRNANPESV
                     PDGMIERFSERVSTPLGSVGDELFWVTLRPHAVLVGSVGALLTGAWGACLLLVVFGVW
                     QGYYRWNSFTWGWAAGARIAAVLQSERFRRPAKYAGWIASGCAGAAVALLLVNSPAGA
                     LTAKAQVVAFGGAFAGACAALSQRKPATWALLAAVGWAIVVSFGAKMLRG"
     gene            8257..8538
                     /locus_tag="P5179_03575"
     CDS             8257..8538
                     /locus_tag="P5179_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRS94332.1"
                     /translation="MATHQVVEADVLVKDREGIHLRNAAALVRAAAQFPKADLMISKD
                     GHQVNGKSIMGVLTLVAEHGAQLHLKARGEEADKLIATLVAMFENGTDQ"
     gene            8599..10407
                     /gene="ptsP"
                     /locus_tag="P5179_03580"
     CDS             8599..10407
                     /gene="ptsP"
                     /locus_tag="P5179_03580"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRS94333.1"
                     /translation="MDPRLPPIEPATLTPHGHCRIVLTGIAGSPGVAYGPVYLHAGAD
                     VWVDNRPIPSEAVESEIARFIAALAAVVEEKRELKRRAEREIGTAQGQIFESHIMLLE
                     DADAIAQTVECVRASKKNVAYCYFVTLQRVIDTIVGTGSYEYLRDRVVDIEDVRAAVL
                     MKLRGAEPQGLDKLEDVSVVVAHSLTPSDTARMEASKVVGFVTDRGGPTSHASILARS
                     MGIPAVVGCQGASVTISPGEMAVVNGFTGEVYVEPDEVLREQIAQWKQQLEQRARRLL
                     EISRLPSQTRDGRRIRLELNIELDSEIQRTAELASDGVGLFRTEFLFLSSDEWPTEEE
                     QYAVYRHLAEAFGGRPVTIRTMDIGGDKLCRKLHVTPEMNPFLGWRAIRISLAQPELF
                     GVQLRAILRASAHGNVQMMFPMISSVEELDQALMILGQAKEALRRNGTPFNDSMPVGV
                     MIETPAAVMIGDALAKKAAFFSIGTNDLIQYALAVDRDNQRVAKLFDMFHPAVLRLIH
                     AAVKAGHLARIPVEVCGEMAHVPLGALLLVGLGVDALSMAPGSIPEIRRIIRGITFAE
                     VKEVARAALDSVSGEEVRRNLERAAADFGLDIGVES"
     gene            10429..11586
                     /gene="metK"
                     /locus_tag="P5179_03585"
     CDS             10429..11586
                     /gene="metK"
                     /locus_tag="P5179_03585"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRS94334.1"
                     /translation="MMPRNMLFSSESVAEGHPDKVADQISDAILDAMLVQDSKSRVAC
                     ETLVTTGLALVAGEITTKAYVEVPDLVRSVIRDIGYTEMGYGFDADGCAVLTAIHQQS
                     PDIAQGVDEGEGKEQGAGDQGMMFGYATDETAELMPMPIMLAHRLVLRLAEVRHSGML
                     PYLRPDGKAQVSVRYENDRPVKVETVIVSTQHNEHVSQQQIREDVISHVVKPVVGEEL
                     LGANTVYHINPTGRFVVGGPHGDTGLTGRKIIVDTYGGFSRHGGGAFSGKDPSKVDRS
                     AAYAARWAAKNVVAAGLAKRCEIQVAYAIGVAKPVSVLVETFGTAQIDESRIEKAVRE
                     VFPLTPKWIISELDLLRPLYRKTASGGHFGRNEFPWEQTNRIAELKTAAGV"
     gene            11798..13345
                     /gene="xylB"
                     /locus_tag="P5179_03590"
     CDS             11798..13345
                     /gene="xylB"
                     /locus_tag="P5179_03590"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRS94335.1"
                     /translation="MRSLVIGVDLGTSGVRAVAVDRSGTVVGSAVRGYQIHAPYPGYA
                     EQDPEEWWRSTCEALRECVAVAGQSTIEGIGFSGQMHGTVLIDPKGGLVRPAIIWPDQ
                     RSGESCRRFVARVGADWLAARTQNSLATGFQLASLLWLSENDPSSLARAFRVLLPKDF
                     IRGRMSGVWATEPSDACSTLAFDSGRCAWADDVIAAAGIRRDLFPECRSSCDSAGCLR
                     EEAAAETGLPAGIRLFMGGGDTPSTALGSGVLSKGQCMVTIGSGGQLVAPLDEPRYDT
                     ALRTNTFCHVVPRTWYAMGAILSAGLSLKWLREKILGPDAPDFARLAASAEHVPPGAE
                     GLIFLPYLTGERTPHMNPDACGVFFGLTPRHTAAHMVRAVMEGVAMALADGLDIIRSL
                     QVTPESVFAAGGGAKSQLWRQILADVFDCPIAVSEERERAAIGAAMVAATGVGWFENL
                     EKASDAWRAPVKDVATPQAGQVERYRELRALFRQVYRSLEECMDARAESLRNRQGNPD
                     LFYGSSE"
     gene            13342..>13691
                     /locus_tag="P5179_03595"
     CDS             13342..>13691
                     /locus_tag="P5179_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94336.1"
                     /translation="MKTPLKVTISPSHPLLILMSPGGPSAEMAAEGFRDEEAVMVRCW
                     ELLDDEIRPCTTVHFGATRGDNFAHADRLLRAAQTAGIPVTLQTQTDNANIQDAMPPE
                     RVRRFLDTYPCIVGL"
BASE COUNT         2591 a         3884 c         4256 g         2860 t
ORIGIN      
        1 cgccaggacg gcggttcatg gggaaaaata gcagaacaac agaaagccgg tgaacgagag
       61 ccctgcccga tcgccctggg tccgggtggc acgctgcttc tgtcggttag cactgctgtt
      121 tcacccagcc acgataacca tgatggaaca accggttttc actgtctccc gcatgtgctg
      181 agtttcagga tcgctggcgg catccttccg gagccaaagg tgataattcc gcagtggaat
      241 gcggaatacc cgttcaacga acattcgtac cgcggatttt cctcagatgc atcggggaat
      301 gttttcctca cgcaccaggc gtcggatggg tctgaatacg ggcaggcatg gtgttaccga
      361 acgtcagagg gcgattgggt ggcagccggg ttccttcgct tccccatgcg tgggtgctac
      421 cccgcggcgg cggtcaacgg caaagcagtc gccgtgctcg cgataagtga tgaaatcgag
      481 ccgaacgagg aatggcgctc tttcaaaaaa gaaagaactg ccagggactg ggactatgat
      541 ttccggcaat tgttcttcac atggacgccg gacgtaacag cggtcccctt ctctgtgccc
      601 ctcaccattg cgtccgtgga cgagacgtgc ggggtgatca ctcacaccga ccttctggtt
      661 actcctgacg gagatgtgca cgttctttac atcgagcgga acgtgtacca cgatttcgtg
      721 cgcgagcgct tcttccccga tctcccactg tccgtctccc tgaagtacgt acgattgcgc
      781 cgcgggtgtg tggttgagcg ccgaatcctc tgcaaatcct gtgagaagtt tgacaacacc
      841 cgggcggaga ccgccgtcaa aatgggcgcg ccagtaccca cctgggcggt atttcatgca
      901 accggcgccg ggagggtttt cctggtctgg aatcagacag acaacgttgc gagccagaac
      961 ggcatgttcc ttcgccagat catccctcaa tgggatacaa cagcgatttc cctgccggtg
     1021 agaaccccgt tgacccagtt ctttgccgcc tccacccgcg ccgggtgcct tcctgacaac
     1081 cggcttgatt tgtacggaac ggctcccggt gaacaggtca tccgtaccat ccattgcgtt
     1141 gtggaaaccg atcaggcgga ggttacgaga tgacaacgcc ccgagagcgc gttgcacgcg
     1201 ccgtcaattt ccagccgaca gaccgtgtcc cgatcgacct tggcggcatg aaggccacgg
     1261 gcatcgcggt caaagcgtac aacgccgtca agaaacagat cggattgtct agcccgacgc
     1321 ggatctggga tccgaaattc atgatcgccc acgtggaaga ggaaatcagg cagagattcc
     1381 atctggacgt cgtcccgctg gacgtttcct ctgtatcgga tgagatgcgt ccagaatgtg
     1441 aatgggtccc gatggcactt tatgaaggcg cggaaggcct cataccgccc gggacgatga
     1501 tcggagagga cgaccgggga cgctgggtac tcatgggcgc ggatggacgg ccgacggcgt
     1561 tgcgcatgcc tcgtgaggga tactatttcg acgacatttc gtttgatcgc cccggtatgg
     1621 aaatccggcc gtccgagttc caaccggaag ccacgttttc ggatgaacag cttcgcgcag
     1681 tggaggcgag agcagacttc ctgttttccc agacagacta cgcggtcttg ggctggggag
     1741 gcggggtgtg ttttctcggg ttgagcctga tcaccgacag atccagtaac gtgacgatga
     1801 attgccccac ccagtggatg accatgctta tcacggaaaa ggaactgtgt cacgaaatga
     1861 tgggccggtc tgtggatgcg gccatttcac ggctcagtct gcttcacgag gccgtgggga
     1921 accgctgtgt cgcgtggggg attgcggccg acgacgccgg gactcaacgc ggcgagttca
     1981 tccgcccaga cctgtgggcg gaaatgataa agccgcatta cgcgaggctg tgttcgtggg
     2041 tgcactcgca caccgaatgg aaaacattcc tccattcgtg cggttccgtg tatcacctca
     2101 ttcctcacat gatcgaggcc ggagttgata tcctgaatcc tgttcagacc tcggcggcga
     2161 acatggaacc ggaacgattg acgcgtgaat tcggaggaaa actggtgttc tggggtgggg
     2221 ggtgcgatac gcagcatgtt cttggcaacg gctcgcctga agaggtacgc acccacgttc
     2281 gcgaacgtct tgagcttttc aaaacggggg gtgggtacgt gttcactcag gtgcacaata
     2341 tccagccgaa cgtgcctccg gagaacatcg ttgcaatgtt ggacgccgca tacgaatacg
     2401 gcgtgttctg aagattacgt tttgtgctct tttttccgtg cggcggggaa aagtacgttg
     2461 ttgagaataa gtcggtaccc cggtgagttc cggtggaaat tgaggtccgt tggcggatct
     2521 cccaccagat gctggtaatc ctcggggtca tgcccgccaa ggaacgtgaa cgtaccggtc
     2581 ccgacctgtc cgtgaacgta cttcacctca tcttcccctt ccgattcgcc cagtacaatt
     2641 actgatggct tcaagaacct gcgatgaaat cctacggtct ggccgaggaa cccgcgaacg
     2701 tggtcgacat ggttctgtgt cagcatcgcc tcgacggggc tcagtttggc ggagaagtcg
     2761 aaaagcgtga agaaatcggc ctccggcccg cgggggttcg cagcgcgatc atcgtacgta
     2821 tcgatgtccg agaactcgta cacccacggg ttctcttcca gccggaaatt ctcgaacgcc
     2881 atcgttttgg agaaatcgag ccggttgttg tagtccggcg ttaccccgtc cccgtcgcag
     2941 atggcgggga caatgtcaac accctctgca gccagggcaa tgtcaattgt gtcggtagct
     3001 gagcacatgg cgaacaggaa cccgccgttt gccacgtact cccggatcgc gcgtgcgacc
     3061 gccttcttga gctcgcttac cttcgcaaaa ccgagttccc gtgcggttcg ctccaattcg
     3121 gctttctgct gaagaaacca gggtgcggtc tggtagctga tgtagaaccg cccgaattgc
     3181 cccgtgaaat cctcgtggtg caggtggagc cagtcatagt ttctcaactc gcccgcgagc
     3241 acctcgcggt cccagagtgt ggtgtattcg atctcagcat aggtgagggc gagcgttacc
     3301 gcgtcatccc atggctgctt gttgggaggg gtatatacgg caatgcgcgg cgctttgtcg
     3361 agtcgtatca cctccatgtt ttcacgttcg atagtgccgt agatttctgc annnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagcaatc cagcggtacc gccgcaacag
     3541 gttcataggg cccccggttg tgcgcgaagc tccctcaatc tccggcgcgc ctgattgccg
     3601 tacacgctgc cgggatacgt catcagcagc gtttccagcg ctctggtctc ctcgccgcta
     3661 tcctgcaacc ttgtgcgctg gagctccgcg taccgccaga gagcctcttc tccagccgaa
     3721 gatgagtcca gccgcgatgc ggctgcttcc agagtggttg ccgcgaggga gtagtgtcct
     3781 tccctgatct gcacctcggc tacgagcaaa cgcgcccagc cttcgagtgg tgtgccgaaa
     3841 aggcgcgggg ccagtaagag aactgagtcg cggtggcctg tggcgaagag gaactcgagg
     3901 tgcccgtacc gaacccagct aactgaatca tcggcggcct gttcgaggat ctccgccagc
     3961 ctgaggcaat cgttcgtttc atcaccgggc gccgcgtttc ccaccatcag ttcacaacga
     4021 gccctcagtc cgggggcatc gccacgaaac atcgcagcct gtgccaggcg caggtgtgct
     4081 ttcagggact ctggttcttt catcgctgaa tccgcgagca tctgccagaa ggattgcgct
     4141 tcctccagat gccccgcacg caaggcacat tcgccccgtc cgagaatccc ctgacgtctg
     4201 tgtgccggcg tacggcctct cgttgcggcc cagtcaaact gctgttctgc ctgttccacg
     4261 gagtcaagag cgagaaaaat ccttccgagc gcgatacggg cgtcgtcatg gtacgggctg
     4321 aacgggacgg agatgatcca cgaaagcagg ttccgggcgc ggtcctgaaa acccatttcg
     4381 tccagtattc ccgccgcagt gtacgcatat cgctgcgcag atggtgactc cgggtagtcg
     4441 cttgccaggc gctccatcag gaatgttgcc accggagttt gcccgcgggc ctccagctca
     4501 tcgatcatgg tcgcaagggt ggtgccggga ttccgggttt ccttcgcgct gcggaggaac
     4561 aggcgtaccg cgtcctcctg ttgcgctcca agtactgcca tttccgccgc gaaaagcgcc
     4621 ctggcggcat cccgcggcgt gctgtccgag agagcgatcg ccgcgcgcgt aacagtctgc
     4681 atcatggccg gagtgaggct ggctccctga atgatttcgc gcgcctcccg tacgcggtcg
     4741 acatcacccg ccacgaaacc agatgccgct tccgccgtcg catgcgcgtc gccaagcgcg
     4801 agtgcgatgt cgagcagacg gcggttcact acgcgctgtc catctccctg tgcgttgcag
     4861 gcacgcaacc aggtctgcgc gcgacgcagt tgtccccgcg aaacaagcct gtccgctaca
     4921 ccgatgtgga atgcgatcgg atcggaggaa gtgcgggaac cgatgcgcca caccgagtcc
     4981 gcctgaacgg tatacccggt ccggtcgaac acgtcaccga actccaccca tagcactgcg
     5041 tcttccggtg tgtttgcagt ggcttcaccg agccacgcca cggcctcatc aaatctgccg
     5101 ttttccgtca gaagccttgt tcctcgcaac acggcatcgg accgggtggg cgctgcctgc
     5161 catacccgcc ggtaatacgc tgcggcatcc agcgggcggt tcgcatgttc aagagcctgc
     5221 gcctgaacga accagccgat tatctcgcgc ggtggtgtaa ccgccgcagg tgccgccgtg
     5281 tgcagcagaa tcagcaggat ggcgttggcc ccgagcgcgc actcactcca tatacgtgtt
     5341 accacactgg ttctgagtga tactgcacgt agcaagaatg ttacgtccac gatgtacgat
     5401 ccactgagtg aacagggtga cggagaaggc agaagccgca tgtgccgctt tcacgacgtg
     5461 acagaaatca tcagtgatgc cctttgatgt gttgatccca gtactgcatc caggcttccc
     5521 tgtgggcatt tatgacgttc caatccaccg gcagttccgt gatctggatc gaacgcagcc
     5581 atcccggttt ttcctgatcg gagatgtctc tccgcgcagg tatcctgtag aatcgctccg
     5641 cttgatgaag cagcgccttc ttcgtggtga cgaattcata gaatctttcg gcttcagcac
     5701 gatgtggcgc cccgttcacg atggcgatgc cttcgttcaa cacaagcgcc ccactcctcg
     5761 gaatgacaaa gcccatggga acattttgtt cccgtttctg gaggaggatg tccgtcagat
     5821 cccagagtgt gaccagcccc tcctggcgtc cgatcatgag cattagtgtc gccggagacg
     5881 ccgcataggc tttcgtgttc gcgtcaagcc gtcggagcca atcgtatccg cggtcaggag
     5941 tgccgtcctc cgcgaagaat cggtacacca tcgccgcaaa cacgcttttc atcgtgcccg
     6001 attccatggg agagcgaatg atcaccttgc cctcccatcg tttttccagc aggtcgtccc
     6061 aatcctgggg agccgtctgt tccgtaagtc tgtcggagtt gaaacctatt accttaaccg
     6121 tctggaacgt gctgtaccag agcccctcgt cgctgaacga gcccggaggg gcagcctcgc
     6181 gccaagaggg atggtaggga gcaagcaacc cttcccgggc cccctgttca aagagcgccg
     6241 atggccctcc ccaccagaga tcagcctggg gattgttttt ttcgccgcgg agtcgttcga
     6301 tccccacttc cgctcccatc ggataccacg tcacgtccac gtgcgggttc gcctcctcgt
     6361 acatccgttc gtattcgctg agcatttctc tcccgtgagg cgaataaacg acgagcttgg
     6421 tccgttgctc cgaacaggag agattcaggg cggctccgag cgcgacgcac acttcgagga
     6481 catggcggcg gaacgttgac tgagtaagaa gggacataca aagtctccgg aatggaaaac
     6541 ctgccgcgtg ggccaactca ggaggctgac ggcgcgtgaa cttgaaatgt acgcaaccac
     6601 ggacaggccc tcacaggtta gccgcagacg gagatttggt tgactcgttt cggtcgcggt
     6661 ggtacgttta gtgtgccctg aaaaggttat tggtggtttg cagtcgggcg ggagatgata
     6721 acggtctggg actgggtaat cctcgcgctt tttgcggcgc ttgtagcgca ggacacaact
     6781 gccgggcctc agttgctctt ctctgaaccg cttgtcgcgg gaacggtcgc gggtcttgtt
     6841 cttggcgacc tgcggctcgg gcttctcctc ggcaccgcca tccagctcat ctggtgcgga
     6901 gccgttcccg cgggaaccgc cgtctttctc gacatcaacg tcggaacggt atgcgccgtt
     6961 gcagtcgcgc actcggcggg aagcgccccc atcgcggctg cggcaagcat gctctggctg
     7021 attcccgtcg gtcttctggg atgcgccctg acagaggtcg ggatgcgatt ctgcggaaaa
     7081 cttgtggcct caattcagcc ggaatgcgtc tcggctggcg cgctggttct gcggcatggt
     7141 gcgggctggc tgttcgcggg ggcgcgtggt tcggtgacgt ttctggccgg cgccggggca
     7201 ggcgtggcgg tggcaccggt cctggttcgg tttctgggcg ccgcggttga tcccggcgtc
     7261 ctctgggcag gcctgttcgg tgccggtgcg ggttctgcaa ttgcggcgac gtggcgcttc
     7321 agcaaaggca aggccgcttt tctgggcgcg gcaggggttt ttggtgcgtg ggcgggcggg
     7381 ttgttgcctg tctcctggtg aagacacacg cgattgactt cgttgactcg ggtatcgtcg
     7441 tccgaaactg cacctgtcgt gcccctgtgg gtgcgtatgg aagtcgtcct gcgcgcgttc
     7501 tggattcagg caagcctgaa ctaccagagc atgcagggcc tgggaaggct ggtatgtctg
     7561 ctccctgtga tgcgctggct gagcctgccc ggaaaggaat ttgcctcgtt tgtgcggagg
     7621 cacagcgggc ttttcaacag caacccgttc gtcgcaacgc tgggtctcgg cgctctggcg
     7681 cggctcgaat tcgaccggaa cgcgaatcct gaaagcgttc ccgacggcat gatcgagcgc
     7741 ttttcagagc gggtttccac tccgctgggc tctgtcggtg acgagctgtt ctgggtgacc
     7801 ttgaggccac atgccgtcct tgtggggtcg gtcggagcgc tcctgacggg agcgtggggt
     7861 gcctgcctcc tgctggtggt ttttggtgtc tggcagggat actatcgctg gaattctttc
     7921 acgtggggat gggcggccgg tgcgagaatc gccgcggtgc ttcagagcga gcgttttcgc
     7981 agacccgcaa aatatgcggg ctggattgcg tccgggtgcg cgggcgctgc cgtcgcgctc
     8041 cttctcgtga actctcccgc gggcgctctg actgccaagg cgcaggtagt cgcgttcggg
     8101 ggagcgttcg cgggcgcgtg cgcagccctg tcgcagagaa aaccggcgac gtgggctcta
     8161 ctggcggcgg tcgggtgggc aatagtggtg tcgttcggcg ccaaaatgct ccgtgggtga
     8221 ccggtaattg aaggactttt cagagttgga gctgctgtgg caacgcacca ggtagtagaa
     8281 gccgatgttc tggtcaagga ccgggaaggt atccatctcc ggaatgccgc tgcccttgtt
     8341 cgagccgcgg cccagttccc gaaggctgat ctgatgatca gcaaggatgg gcaccaggtg
     8401 aacgggaaaa gcatcatggg agtgcttacc ctggttgccg agcacggcgc ccagttgcac
     8461 ttgaaggcgc ggggagagga agcggacaaa ctgatcgcca ctctcgttgc catgtttgaa
     8521 aacggtacgg accaatgatt catcaaccgg tggtcattct gctgcgtgaa tgccatgcat
     8581 aaggcgtctg acagccccgt ggacccccgg ctcccgccta ttgagcccgc gactctgact
     8641 cctcacgggc actgcagaat cgtacttacg ggaatcgcag gctctcccgg cgtcgcatat
     8701 ggtcccgtct accttcatgc cggtgcggat gtctgggttg acaaccgtcc gattccttcc
     8761 gaggcagtgg aatcagagat cgccagattc attgccgcgc tggcggcggt ggtggaggaa
     8821 aagcgggaac tgaaacgccg tgcggaacgg gaaatcggca ctgcccaggg ccagatcttc
     8881 gaatcccaca tcatgctgct ggaagacgca gacgcaatcg cgcaaaccgt ggaatgcgtc
     8941 cgcgcctcga aaaagaacgt tgcgtactgc tactttgtga cgctgcaacg ggttatcgac
     9001 accatagtcg gaaccggttc ctacgaatat ctgcgtgacc gcgtggtgga tattgaggac
     9061 gttcgtgcgg cagtcctgat gaaattgcgg ggcgcggaac ctcaggggct ggacaagctt
     9121 gaggacgtgt cagttgttgt ggcacattcc ctcacgccgt ctgatacggc gcggatggag
     9181 gcctcgaagg ttgtcggttt tgttaccgac cgtggcggtc ccacaagcca cgcttcaatt
     9241 ctggcgcgtt cgatgggaat ccctgcagtc gtcggttgtc agggagcatc cgtgaccatc
     9301 tcgccgggcg aaatggcggt tgtgaatggt ttcactggtg aggtgtacgt cgaaccggac
     9361 gaagtcctcc gtgagcagat cgcccagtgg aaacagcaac tggaacagcg cgcccggagg
     9421 ttgctggaga tatcgcggct cccctcgcag acgcgagatg gacgccgcat acgccttgaa
     9481 ttgaacattg aacttgacag cgagattcag agaacggcgg agcttgccag tgacggcgtc
     9541 ggtctgttca gaacggagtt cctgtttctt tccagcgatg aatggccgac agaagaggag
     9601 cagtacgcgg tctaccgcca tcttgctgaa gcattcggag ggcgtccggt tacgatccgc
     9661 acgatggaca tcggcgggga caagctctgc agaaaactcc atgtgacgcc ggaaatgaac
     9721 ccgttcctcg ggtggcgcgc gatacgcatt tcgctcgcgc aaccggagct gtttggtgtt
     9781 cagttaagag ctattctgag agcttccgca catgggaacg tccagatgat gttcccgatg
     9841 atctccagtg tcgaagagct tgatcaggct ctcatgatcc tgggccaggc gaaggaagca
     9901 ctccgccgca acggtacgcc gttcaatgat tcgatgccgg taggcgtgat gatcgagact
     9961 cctgccgccg tcatgatagg cgatgccctg gcgaaaaaag ccgcattttt cagcatcggc
    10021 acgaatgatc tgatccagta tgcccttgcc gtagaccggg acaatcaacg tgtcgcgaag
    10081 ctcttcgata tgttccatcc ggctgtgttg cggttgatcc acgcggcagt gaaagcgggg
    10141 caccttgcac gcatacctgt cgaagtgtgc ggcgaaatgg ctcacgtgcc actgggtgcg
    10201 ttgttgctgg tgggtctggg agttgatgcg ttgagcatgg cccccggaag tattccggaa
    10261 attcgtcgca tcatccgcgg gattacgttt gcggaggtga aagaggttgc gcgcgcagca
    10321 ctcgattcgg tttccggtga agaggtacgg cggaacctgg aacgtgctgc tgccgatttt
    10381 ggtttggata taggtgtgga gtcgtgagtt tcttttcgaa caggagacat gatgcccagg
    10441 aacatgcttt tttcttcgga atccgtggct gaagggcatc cggacaaggt ggcagatcag
    10501 atttccgacg ccattcttga cgcgatgctg gtccaggact cgaaatcgag agtggcgtgc
    10561 gagacccttg tcactaccgg tctggcgctg gtcgccgggg agatcaccac gaaggcatac
    10621 gtggaggtcc ccgatcttgt gcgctccgtg attcgcgata tcgggtatac ggagatgggg
    10681 tacggattcg acgcggacgg ttgtgctgtt ctgacggcga ttcaccagca atccccggat
    10741 attgcccagg gagttgacga aggtgaaggg aaggaacagg gtgcgggcga tcaggggatg
    10801 atgttcggat acgctaccga tgaaactgcg gaactcatgc cgatgccgat catgctggcc
    10861 catcgcctcg tgcttcggct cgcagaggtg cggcacagcg gaatgttgcc ttacctccgg
    10921 ccagacggga aggcgcaggt ttccgtgcgg tacgaaaacg acaggccggt gaaagtcgaa
    10981 accgtgattg tctccaccca gcataacgaa cacgtctccc agcaacagat tcgggaagac
    11041 gtgatctcgc atgtggtcaa gccggtcgtg ggcgaggaat tgctgggagc gaatacggtg
    11101 taccacatca atcccaccgg ccgatttgtg gtcggcggcc cccatggaga tacgggtctg
    11161 accggtcgga aaatcatcgt ggatacgtac gggggattca gccgacacgg tggcggtgcg
    11221 ttcagcggca aggacccgag caaggtggat cgaagcgctg cgtatgcggc tcgttgggcg
    11281 gcgaaaaatg tcgtggccgc aggtctggca aagcgctgcg agattcaggt tgcgtacgcg
    11341 atcggcgtcg ccaaacccgt gagcgtgctc gtggaaacgt tcggcaccgc tcagatagac
    11401 gaatcgagaa tcgaaaaagc agtgcgcgag gtgttccctc tgacacccaa gtggatcatt
    11461 tcggagctgg atctcctgcg cccgctttac cgcaaaacgg cttccggagg gcacttcggg
    11521 cggaacgagt tcccatggga acaaaccaac cgcatcgcgg agctcaaaac tgccgctggc
    11581 gtctgatggt tctggtcatt cgagaggaaa tcacgggcac cggtgcacct cgcgtcggtg
    11641 ccctttgatt tggttgcaat gacggcgttg cccccgcgta aggtttgagg gattcttcat
    11701 ctgttcgcac ttcaggaatc ctgcccagtg aaagacaacg gttctgcagc gcgaaatact
    11761 ggcaagattc ggccgctcag atcctggaaa aagacacatg cgctccctcg tgattggagt
    11821 ggacctggga acttccggcg tccgggcggt tgcagttgac cggtctggaa cggtggttgg
    11881 tagcgcggtg cgcgggtatc agatccacgc accatacccc ggatacgcgg aacaggaccc
    11941 ggaggaatgg tggcgctcga cgtgtgaagc gctgcgtgaa tgcgtcgccg tggccgggca
    12001 aagcactatc gagggcatcg gtttctccgg gcagatgcac ggaacggttc tgattgatcc
    12061 gaagggcggg ctggtacggc ccgcgatcat ctggccggat cagcggtcgg gagaaagctg
    12121 ccggcgtttc gttgccaggg tcggtgccga ctggcttgcc gcccgcacgc agaactcgtt
    12181 ggccaccggt tttcagctcg cgagtctgct ctggttgtct gagaacgacc cgtcttcgct
    12241 tgcgcgcgcg ttccgtgtgc tcctcccgaa agacttcatc cgaggccgga tgtccggcgt
    12301 gtgggcaaca gaaccctccg acgcgtgcag tacgctcgcg ttcgactccg ggcggtgcgc
    12361 gtgggctgac gacgtgattg ctgctgcggg cataaggcgt gacctgtttc ctgaatgccg
    12421 cagttcgtgc gactctgccg ggtgcctgag agaggaggcg gcggcggaaa ccggtctccc
    12481 tgccgggatt cgtttgttca tgggtggcgg ggatacaccc tcaacggcgc tcggttcagg
    12541 agtgctgtcg aagggccagt gcatggttac gatcggatct ggtgggcagc ttgttgcacc
    12601 actcgatgaa ccgcggtacg atacggctct gcgcacaaac acgttctgcc acgtggttcc
    12661 gcgcacgtgg tacgccatgg gcgccatcct ctcggccggg ttatccctca aatggcttcg
    12721 tgaaaaaata ctggggcccg atgcgccgga tttcgcccgc cttgccgcct ctgcggagca
    12781 cgttcctccg ggtgctgagg ggctcatatt ccttccctac cttaccggtg agcggacccc
    12841 gcatatgaac cccgatgcgt gtggcgtctt cttcggcctg actccccgtc atacggctgc
    12901 gcacatggtg cgcgcagtga tggagggtgt ggcaatggcg ctcgcagacg gactcgatat
    12961 catccggtcg ctccaagtaa ccccggaatc ggtgttcgca gcaggaggcg gcgcaaaaag
    13021 ccagctttgg cggcagatcc tggccgacgt gttcgactgc ccgatcgctg tgagcgagga
    13081 acgggaacgc gccgcaatcg gtgcggcgat ggttgcggcg acgggagtgg ggtggttcga
    13141 gaacctggag aaggcttccg acgcctggcg agcaccggtg aaagacgttg cgacaccaca
    13201 ggccgggcag gttgagcgct accgtgaact gcgcgcgttg ttccgccagg tgtacagatc
    13261 gctggaggaa tgcatggacg cgcgcgcgga atccttgcgc aatcgacagg gcaatccaga
    13321 cctgttctac gggagttcgg aatgaaaacg ccactgaaag tgactatttc accctcgcat
    13381 ccccttctca tcctcatgtc tcctggaggc ccttccgccg aaatggctgc cgaaggattc
    13441 cgggacgagg aggcagtcat ggtgcggtgc tgggagctcc ttgatgacga gataagaccc
    13501 tgtacgaccg ttcatttcgg cgcgacacgc ggagacaact tcgctcacgc tgaccgcctc
    13561 cttcgtgcgg ctcagacggc gggcatcccg gttacattgc agactcagac ggacaatgcc
    13621 aatatccagg atgcgatgcc gccggaaagg gtcaggcgct ttctggatac ctacccctgc
    13681 atcgtcggcc t
//
LOCUS       DAOTNG010000046        13589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4418_length_13589_cov_5.075587, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000046 DAOTNG010000000
VERSION     DAOTNG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_13589_cov_5.075587"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1285)
                     /locus_tag="P5179_03600"
     CDS             complement(110..1285)
                     /locus_tag="P5179_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94337.1"
                     /translation="MTKSKLRRIGVGMLGYGFMGKVHTECYRMLRMWCETLPVEVHFV
                     GVATKHVETAEFAVEQAGYGFGTTDWRQVVEHPDVDVVNICTPNALHFEQAMGAIQSG
                     KHVYCDKPLATNPDHARTMANAAQAAGIVHQVTQNYRFVPATLRAKQLVDEGLLGRPY
                     SFRAAYLHSGSSDPERPAGWKMQDGGTLHDLGAHVVDLVRHLWGDFAEVLAHRQVFVP
                     SRPIRAGSSERVAISGDDAAWMLTKLANGAIGTIETNKVATGTEDELRIELHGEKGAI
                     RFNLMEPNWLDVYDARAEDGPYGGNRGFLRIATCSRQKEPSVIPPPRGAAGWMQFHLA
                     SLHSFVGNVLRGSAGHPDFFDGWAVHEVLAAAEESSRTGTWVNVRSVNCRAEAEEQR"
     gene            1445..2338
                     /locus_tag="P5179_03605"
     CDS             1445..2338
                     /locus_tag="P5179_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS94338.1"
                     /translation="MREVRWERMFPDELEKAFSECPVVYFAYGLCEPHGPQNAIGLDA
                     LKAHAICCRAAERHGGIVAPCDFWHIHELGGYAVWSRQAIGEVERSWLTCMPPWVHLK
                     NVCYHVRQADVLGFHAAILFTGHYGPNWEDLKTLVRLIQPYVGTRLYSLPECEANVPG
                     FDGDGASGDHAGKVETSLLWALMPECTDVSRLPDKETGPAPWAMGRNAYEASRRIGER
                     MVEDEVTWLGRKAGELLEEYEKSRPSHTLRTFEDVERLWEGVVRPHVPEFRSMQLCWK
                     EHQEVPGDSVWYSNWKVPERA"
     gene            2547..5144
                     /gene="alaS"
                     /locus_tag="P5179_03610"
     CDS             2547..5144
                     /gene="alaS"
                     /locus_tag="P5179_03610"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRS94339.1"
                     /translation="MTSSELRRGFLDYFAKRGHRIVPSSPLIPHDDPTLLFANAGMNQ
                     FKDIFLGQRVPEFRRAASSQKCIRAGGKHNDLEDVGKDGTHHTFFEMLGNWSFGDYYK
                     AEAIEWAWEYLTSELQLPKDRLRATVHHSDDEAEELWAKIAGIPRSHISRHGDKDNFW
                     EMAEVGPCGPCTEIHLDMGADRGCGRPDCGPNCKYCDEVHDARFMELWNLVFMQYYRD
                     ENGVLTPLPETHVDTGAGFERLCSVIQGKSSNYDTDLFMPIIRATEEKAGREYISGVE
                     GTPFRIIADHMRALTLAITDGGFPSNVGRGYVLRRILRRASRAGRQLGLREPFLYELT
                     NSVVDTMGSAYPELEERRTHVATVIKREEEQFGRTLNRGIDQFETFAAELAATGSKVL
                     DGEKVFQLYDTYGVPVDLTALMAEDMKLTIDLDGFNRCMEEQRERSRAQAKFDRAVTN
                     FGEGLVSEFVGYDTLSTEAQITGLAEGAIVLDQTPFYAESGGQVADVGELFNAEFSFE
                     VTGVQKQGTAIVHFGKVTRGAPSVGMAVTAQVNSDLRGRTMRNHSATHLLQAALRAAL
                     GPQVHQAGSFVSPDRLRFDFTCGEAVPPEVLRQVEATVNARIRENLAVNISHSTLDEA
                     KASGAIALFGEKYGETVRVVRMGNFTMELCGGTHVDATGQIGYFRILSESGVAAGTRR
                     IEAVTGVTAEELAREDRDRLARISAILKVGVDDVEPRITALLDRLKEIERELEKAQTA
                     MAGADVDAWIREAGEIGDARLLIREVTLADPKAFRALGDTVREKLGKGVALLIGHDAG
                     KLSLMLLVTDNLTSRIQAGKLMNELAAIVGARGGGRAHMAQAGGGSPERLADLLAKAP
                     GVVARELGI"
     gene            5264..6184
                     /locus_tag="P5179_03615"
     CDS             5264..6184
                     /locus_tag="P5179_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRS94340.1"
                     /translation="MSGIAFVLLLLGSAQADTILSRGSSNEWCWRCHGMATFSVLRAD
                     GTIRTLSADSTAFLASNHRRLRCVTCHSWEDVQVFPHGKNAHMAQTCTDCHVAESHPL
                     NRTDPLQAMHFREITDEFRKSVHYQTEDHAFSCFSCHDPHRFRKSENPSLQTIGTQNG
                     VCLGCHGDAARFATLSERRIDLSATHAWLPRRELHWEHVRCLDCHTSYAPPNLSHNIL
                     PADSAVHACEQCHRQNSILLDKLYRYRHVEERSRNGFVNAALVNDSYVIGSTRNRYLD
                     LLGAVLVGGACVGVAGHGFGRWHSAGKKRK"
     gene            6193..6861
                     /locus_tag="P5179_03620"
     CDS             6193..6861
                     /locus_tag="P5179_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRS94341.1"
                     /translation="MDTVHREYLHPLWVRVWHWLNAALFLALAVSGFSLHFSGSAVQL
                     LPFRTAILVHNASGALFTIALILYAILLGITGEWRQYLGLRPRMLALVGQNLRFYLID
                     VFKGAPHPESPTRTQKFNALQQVTYLFVLVIGMVPVAITGVMLSVPETAPKQILGAGG
                     IWPVAVLHTLLAYGLVAFFVGHVYLTTMGPTLWSDIKSMFTGWHERVHGQADIQPQEQ
                     GDTP"
     gene            6858..9125
                     /locus_tag="P5179_03625"
     CDS             6858..9125
                     /locus_tag="P5179_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HRS94342.1"
                     /translation="MTDTPQRTSNESFPCPGWWKRAFRNRRLRKWSFGILVAVGCGLA
                     IRYVLVPWGFQKYTGVVNAELSYPNSKAVSEAEFEAIAADLRSEGILRRALALTSSVT
                     DSITRKTRIRMIAATPSFQRQEEFAHIRYFRDAGIRRYEGPKTCIRCHETIDVRKPDG
                     TIQKVKTIPDVLNTIHYKFQSMDGGFTTYGYDGRQVNTGTRGIPVGKIDRACGVPGSF
                     SWTGWAALIETKPAHAGGEVVLRSEGCGQCHIGGGYHPATENMMPGLVVPTPKVAAEG
                     LDCLICHASAYDMNQRFVIRDEHGLRWNQDRSLRAALSVGRPTNENCLNCHQHNMGGD
                     TYAYNTSAQSLGTQNQRLLHSTAKRGNPFSVHDDVHARAGLVCLDCHRPQGHKIPRGN
                     KGTDLVANDLPDVKVECEMCHTSAPHVRNRRTRAALNGHADYIACETCHIARLLPFNN
                     VLKDWVHPVWNEEEGMYVPKAVYSSGDPNRGLAYLWFNGNGTFLANALGANPNHSPDY
                     NPLMRQIVMIEDPVVLGEIAANTRDIRTRYGLDSASYMAPIANALSQLSPEMLSRRRE
                     MIERNLRVRMNEGKSRIYPFKLFNAMMFEDLNNEGPFGAMILPFDYRTYFETGDAENA
                     VKVAVANPIVKRMYETPFKLYMMDEFMAYFGVGKWTAKYPLDEGNWNVQPRWMRQMGT
                     LMVNHGIQPVGRQCAECHNRNGILDFAALGYTTDRVRALQNLPELSYFQPPLRPSHRQ
                     EGAEVQAEATDASER"
     gene            9109..10569
                     /locus_tag="P5179_03630"
     CDS             9109..10569
                     /locus_tag="P5179_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRS94343.1"
                     /translation="MLQNDKIWVGKGANPVYLLPTMANRHGLIAGATGTGKTVTLKVV
                     AEAFSDMGVPVFAADIKGDLAGIAVAGTPNPKVEERLRKLGVEGFQYAGFPVRFWDLF
                     GEAGHPVRATVSDMGALLLSRMLGLNDTQAGVLNITFRVADDNGLLLLDLADLRAMLQ
                     YVGDHASELTTRYGNVSIQSVGAILRALLTLEDQGGAKFFGEPALDISDWMQVDAQGK
                     GYINILHSVKLYMSPALYSTFLLWMLSELFEVLPEAGDLQKPKMVFFFDEAHLLFKDA
                     PKALLEKIQQVVRLIRSKGVGVYFVTQVPSDVPQEVLGQLGNRVQHALRAFTPADQKF
                     VKAAARSFRANPSLDSEKTILELGTGEALVSFLDADGKPSIAERAMILPPQSSFSAID
                     DSTRSRIISASLLGRKYETAVNRKSAFETLQAESQAPREAPRERTTTPKRVPTGTTGR
                     RTRTPLEAAAGSMMTTIGREVGRQIIRGILGSILKR"
     gene            10664..11440
                     /gene="hisF"
                     /locus_tag="P5179_03635"
     CDS             10664..11440
                     /gene="hisF"
                     /locus_tag="P5179_03635"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRS94344.1"
                     /translation="MLTKRIIPCLDVRAGKVTKGVAFQGNIDVGDPVAMAKHYYEQGA
                     DEIVFYDITASNERRDIMIDVVASVAREIFIPFSVGGGLRTLSDMRRVLLAGAEKVSI
                     DSGAVRNPDTIRQGAEAFGRQCIVLSMQVKRVPQTPTIPSGYEIYIDGARTATGLDAI
                     EWAQRGEELGAGELVVNSIDADGVKTGYDVDLTRRMAEAVGIPVIASGGAGEPSHLYD
                     VFSQGNADAAIVASMIHFGTYSIPEIKEYLHRRGLPIRMV"
     gene            complement(11505..12287)
                     /locus_tag="P5179_03640"
     CDS             complement(11505..12287)
                     /locus_tag="P5179_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HRS94345.1"
                     /translation="MATKPPVAVVTGAGRGIGRGIALGLAKANFDVAVNFAHNAEAAA
                     ETCEEISRLGRHAMPVRADIADADDRAQLIAETENRLGPVTLLVNNAGIAPEVRADVL
                     ESTEESFDRLVRTNLKGPYFLTQIVATKMIEWQRAGLVREPKIVFITSVSAFAPSVNR
                     GDYCISKAGLAMAAKVFAARLAGDGIGVYEVQPGVIQTDMTEKVRERYDKLILEGNLL
                     PIKRWGQPEDVAHAVVAIAEGRFSYSTGITIVVDGGFHLRVL"
     gene            complement(12318..13229)
                     /locus_tag="P5179_03645"
     CDS             complement(12318..13229)
                     /locus_tag="P5179_03645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRS94346.1"
                     /translation="MSSPFSIPRLDDLAGCYRYCREITHLYGANFSVGFRCLPKRKRD
                     AVYAVYAFCRFADDIADEYTGHENPEALLDAWEKALERCYQGVTDHPILVALADAAAR
                     FPIPLESFRRLIEGCRMDFVKTRYATFEELLVYCDRVATTISELSLAIFGWTDPRTPR
                     WGTELSIALQMTNILRDVAEDARRGRIYVPQEDLVRFGCREEDLKSREPAPGFLQLME
                     FELERVFRYFDLARPLCGAVSPDSRMAVALMGSVYREVALQISRNTHSVLVGKVQLTT
                     LQKLVLVVNAAARSAFHRLGVSGPPCW"
     gene            complement(13239..>13589)
                     /locus_tag="P5179_03650"
     CDS             complement(13239..>13589)
                     /locus_tag="P5179_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94347.1"
                     /translation="ADHLFGFGIRLALQSGVLAAQCVLSGRAFGPAWEEHFGQRFEMG
                     LANRFLYETGGHVAYRWFLAKASRSDFREVGQALYKPTAAKRALATIARAVWKRRPEC
                     VHGARCIWCRLPKG"
BASE COUNT         2866 a         4018 c         4026 g         2679 t
ORIGIN      
        1 tgacccgcgc cgatcccgcc cagcggcatg gcgactgctc tcaatgcctt gccgagaatc
       61 acgcgacgat cgggtctgcc agccagagcc tccggcaatg tgtttccact catcgttgtt
      121 cctccgcttc agcccgacaa ttcacagatc gcacgttcac ccatgttcca gttcgactcg
      181 attcctccgc cgcggcgaga acctcgtgca cagcccagcc atcgaagaaa tcgggatgcc
      241 ccgcactccc tcgcagaacg tttcccacga acgagtgcag gctggcgaga tggaattgca
      301 tccacccggc cgcgccgcga ggcggcggta tcaccgaagg ttccttctga cggctgcagg
      361 tggcgatgcg gagaaagcct ctgttcccgc cgtacggtcc atcttcggca cgcgcgtcgt
      421 acacgtcgag ccagttcggc tccatgaggt tgaaccggat tgctcccttt tccccgtgga
      481 gttctatccg cagttcgtcc tccgtacctg tggcaacctt gtttgtctcg attgtgccga
      541 tcgctccatt tgcgagcttc gtgagcatcc acgcggcgtc atctcccgat atcgcgaccc
      601 gctccgaaga gcctgctctt atcggacgcg acggcacgaa aacctgccgg tgcgccagaa
      661 cctcggcgaa atctccccac agatgacgaa ccagatccac aacgtgcgcg cccagatcgt
      721 gcaacgtccc gccatcctgc attttccatc ctgcaggccg ttccgggtcg ctggatccgc
      781 tgtgcaggta cgccgcgcgg aagctgtacg gtcttccgag cagtccttcg tccacgagtt
      841 gtttggctcg cagagtggcc ggcacgaatc gatagttctg cgtaacttgg tggacgatgc
      901 ccgccgcctg tgctgcgttc gccatggtcc tcgcgtggtc ggggttcgtg gcgaggggtt
      961 tgtcgcagta cacgtgtttg cccgactgaa tcgcgcccat cgcttgctcg aaatgcaggg
     1021 cgttaggcgt gcaaatgttc accacgtcca catccggatg ctcaacaacc tgccgccagt
     1081 ctgtcgttcc aaacccatac ccggcctgtt ccacggcgaa ctcggcggtt tcgacgtgtt
     1141 tcgttgccac accgacaaag tgcacttcca cgggcagcgt ctcgcaccac atccgaagca
     1201 ttcggtagca ctcggtgtga actttgccca tgaatccgta cccgagcatg cccaccccga
     1261 tacggcgaag ttttgatttc gtcatatgga acctcctgtc gaacgattcc ctgaaaggta
     1321 ggcgttgtgc tgttcccgct caaaccaaga ggcccctctt gctcccgcct cagttgtgtg
     1381 gcattatccg gctatcaaag gggctggacc tgattgattc ggaaactcaa agggaggcat
     1441 cgccatgcgc gaggtacggt gggaacggat gttcccggac gagctggaaa aggcgttctc
     1501 tgagtgcccg gttgtgtatt tcgcatacgg actttgcgaa ccgcacggtc cccagaatgc
     1561 aatcggtctg gatgcgctca aggcgcacgc catctgttgc cgagccgcag aacgacatgg
     1621 cggcatcgtc gcaccgtgcg atttctggca catccacgaa ctcggggggt atgccgtgtg
     1681 gtcgcgccag gccatcggcg aggtggaacg aagctggttg acctgcatgc cgccgtgggt
     1741 gcatctcaaa aacgtctgct accacgtgcg acaggccgat gtcctgggtt tccacgcggc
     1801 tatcctgttt accggacatt acggaccgaa ctgggaggac ttaaaaaccc tggttagact
     1861 gattcagccc tacgtcggga cccgcctcta ttcattgccc gaatgtgaag ccaatgttcc
     1921 cggttttgat ggcgacggag cgagcggcga ccatgccggg aaagtggaaa cctcgcttct
     1981 ctgggcactt atgccggaat gcacagacgt gtcccgtctg ccggacaagg aaaccggccc
     2041 cgctccgtgg gcaatgggga gaaacgcgta cgaagccagt cggcgcatcg gagaacgcat
     2101 ggtggaggac gaggtcactt ggctgggcag gaaagccggc gaactcctgg aggagtacga
     2161 aaaatccaga ccatcgcaca cactcagaac gtttgaggat gtcgaacgcc tctgggaagg
     2221 tgttgttcgc cctcacgttc ccgagttccg ttccatgcaa ctctgctgga aggaacacca
     2281 agaggtgccc ggggacagcg tgtggtactc aaattggaag gttcccgagc gtgcgtgatt
     2341 ccatcggggt tgctcatgtt ttcgttcgac gcccgcggga gacacccgtt cagacgtcct
     2401 ctggaacacg ttactggcgg tcccctttcg ccccggggtg aaaggggaca ttcgccatcc
     2461 aggcattcat ttgcgatatt tatgtctctc gcgggcggcc ccgggatttc gtctttcatt
     2521 tccaactcat tgcacgagaa ttgtctatga cttcaagtga actccgccga ggctttcttg
     2581 actacttcgc caaacggggc catcggatag tcccctcgtc ccctttgatc ccgcacgatg
     2641 atcctacgct gttgtttgcc aacgcaggga tgaaccagtt caaggatatc ttcctcgggc
     2701 agcgggttcc ggaattccgc cgcgcagcct cctctcagaa atgcattcga gcgggaggaa
     2761 agcataatga tctggaggac gtagggaagg acgggacgca tcacaccttt ttcgagatgc
     2821 tcgggaactg gtcgttcggc gattactaca aagcggaagc gatcgaatgg gcgtgggagt
     2881 acctgacctc ggaacttcaa ctgccgaaag accgtctgcg ggcaaccgtt catcattcgg
     2941 acgatgaggc cgaggaactc tgggcgaaga ttgccggaat acctcgatcc catatctccc
     3001 gccacggtga caaagacaat ttctgggaaa tggcggaagt cggcccctgc ggcccctgca
     3061 cggaaattca cctcgacatg ggcgccgacc gcgggtgtgg ccgcccggat tgcgggccga
     3121 actgcaaata ctgcgatgag gtgcacgacg cgcggttcat ggagctatgg aacctcgtgt
     3181 ttatgcaata ctatcgcgat gaaaatggtg ttctgacgcc actgccggaa acgcacgtag
     3241 acacgggagc aggtttcgag cgactctgct cagtgatcca gggcaagagc tccaactacg
     3301 acacagacct gttcatgccg atcattcgcg caacggaaga gaaagccggc cgcgagtaca
     3361 tctcgggagt ggaaggcaca ccgttccgca tcattgccga ccacatgagg gctctcacgt
     3421 tggccatcac cgatggcgga ttcccgtcca acgtgggaag aggatacgtt ctccgccgga
     3481 tcctgcgccg cgccagccgc gccggtcgtc aactgggatt gcgtgaaccc tttctctacg
     3541 aactgacgaa ttcagtcgtc gacacgatgg gaagcgcgta ccctgaactg gaagagcgcc
     3601 gtacccacgt cgccacagtc atcaaacgtg aagaggagca gttcgggcgt acgctgaacc
     3661 gtggcattga ccagttcgaa acgtttgccg ccgaactggc ggcaaccgga tcgaaagtac
     3721 tcgacggcga gaaggtattc cagctttacg acacctacgg tgtgcccgtc gacctcaccg
     3781 cgcttatggc tgaggatatg aagctgacca tcgaccttga cgggttcaat cggtgcatgg
     3841 aagagcaacg ggaacgatca cgcgctcagg cgaagttcga tcgagccgtc acgaatttcg
     3901 gcgagggtct cgtttcggaa ttcgtcggtt atgataccct ttcgacggag gcacagataa
     3961 cgggtctagc ggaaggggcc attgtactgg accagacgcc attttacgcg gagtctggcg
     4021 gacaggtggc tgatgtaggg gagttgttca acgcggagtt ttcgttcgaa gtgacggggg
     4081 ttcaaaaaca gggaaccgcc atagtgcatt tcggaaaggt gacgcgcggg gcgccctccg
     4141 tcggaatggc cgtaaccgct caggtaaaca gtgatttgcg cgggcgcacc atgcggaacc
     4201 attcggccac tcatcttctc caggccgcgc ttcgtgccgc cctggggccg caggttcatc
     4261 aggcggggtc tttcgtatcg ccagaccggt tgcggttcga tttcacgtgc ggggaagcgg
     4321 ttccgcccga ggtattgcgc caggttgaag caacggtgaa tgcccggatt cgtgagaacc
     4381 tcgccgtgaa catctcgcac tcaacactcg acgaagcgaa agcctcagga gccatcgccc
     4441 tgttcggtga aaaatacggt gaaacggttc gcgtcgtgcg catgggcaac ttcaccatgg
     4501 agctgtgcgg aggaacgcac gtggacgcca cgggacagat tgggtacttc cgaatcctct
     4561 ccgagagtgg cgtcgccgcg ggaacccgaa ggatcgaagc agtaacagga gtgacggcag
     4621 aggaattggc ccgagaggac agagaccgtc ttgcacgaat cagtgcaatt ctcaaagttg
     4681 gcgtggacga cgtcgagcca agaatcacgg cactgctcga tcggctgaag gaaatcgagc
     4741 gggagctcga aaaagctcag acggcgatgg cgggggcgga cgttgatgcg tggatccgcg
     4801 aagccggtga aataggtgac gccaggctgc tcattcgaga ggtcaccctc gcggacccga
     4861 aagccttccg tgccctcggc gacacggtga gagagaaact gggcaaaggt gtggcgctgc
     4921 tcatcgggca tgatgccggc aagctttccc tgatgctgct ggttaccgac aacttgacct
     4981 ctcgcattca agcgggtaaa ctcatgaacg agctggctgc catcgtcgga gcgcgcggcg
     5041 gcggcagagc tcacatggca caggccggag gaggctctcc cgagcgtctg gcagacctcc
     5101 tggcaaaggc acccggggtg gtagcccgcg aactgggcat atagagggaa gggaagcaag
     5161 aacgctcttg cttctcgcgc aacgccttgg acattatccc atcgccgccc acaggcgagt
     5221 gcgtccggcg gcaacgggat aacaccttcg ccgagggctc cttatgtcag gcattgcctt
     5281 cgttctcctg cttctggggt cggcacaggc cgataccatc ctgtctcgtg gttcttcaaa
     5341 cgagtggtgc tggcgttgtc acggcatggc gaccttttcc gtgctgcggg cggacggaac
     5401 cattcgaacg ctgtcggcag attcgaccgc gtttctcgcc tccaatcatc ggcgccttcg
     5461 ctgtgtaacc tgccactcgt gggaagacgt tcaggtgttc ccccacggga agaacgccca
     5521 catggcgcag acgtgtaccg attgccacgt ggcggaatca catcccctca accggacgga
     5581 cccgcttcag gcaatgcact ttcgggaaat cactgacgag ttccgaaaaa gcgtccatta
     5641 ccagactgaa gaccacgcgt tcagttgttt ctcatgtcat gatccgcacc ggttcagaaa
     5701 gtctgaaaac ccgtcgcttc agacgattgg aacacagaac ggggtttgtc tcgggtgcca
     5761 cggcgatgcg gccagattcg ccacgctctc agaacgtcgg atagacctga gcgcgacgca
     5821 cgcgtggctg ccgagacggg aactgcactg ggagcatgtc cgctgccttg attgccatac
     5881 ctcgtacgca cctccgaatc tctcacacaa tatccttccc gcagacagtg cagtgcatgc
     5941 ctgcgagcaa tgccacaggc agaactccat ccttctcgac aagttgtacc gttaccgcca
     6001 cgttgaagaa agaagcagga acggctttgt caacgcggcg ctggtcaacg attcctacgt
     6061 catcggttcc acccgaaacc ggtatctgga cctcctcggc gcggtccttg ttggcggtgc
     6121 atgcgtcggg gtagccgggc atggattcgg ccgctggcac agcgcgggaa agaagcggaa
     6181 gtgaggacgc agatggacac cgtacaccgc gagtaccttc accccctgtg ggtgcgcgta
     6241 tggcactggt tgaacgccgc gctgttcctc gccttggcgg taagcgggtt cagtctgcat
     6301 ttcagcggca gcgcagtgca gttgctgcct tttcgtaccg cgatcctcgt gcacaacgcc
     6361 tcaggtgcgc tgttcacaat tgccctgatc ctttacgcca ttctcctcgg aatcaccggg
     6421 gaatggcgtc agtacttagg gctacgcccg cggatgctcg cgctcgttgg gcagaacctt
     6481 cggttctacc tcatcgacgt gttcaaaggg gctccccatc ccgaatcacc gacgcgaact
     6541 cagaagttca acgcattgca gcaggttacg tatttgttcg tacttgttat cggcatggtg
     6601 cctgttgcca ttaccggcgt catgctgagc gtccctgaaa ctgcaccgaa gcagatcctc
     6661 ggagcggggg gaatctggcc tgttgccgtg ctgcatacat tgctggcgta cggactggtt
     6721 gcgttctttg tcgggcacgt ctacctgacc acgatgggtc ccacgctgtg gagcgacata
     6781 aaaagcatgt tcaccggatg gcacgaacgt gttcacgggc aggctgatat ccagccccag
     6841 gaacagggag acacgccatg accgacaccc ctcagcgaac ctcgaacgaa tcattcccct
     6901 gccccggatg gtggaaacgt gcgttccgga accgacgcct gaggaagtgg agcttcggaa
     6961 ttctggtcgc cgtggggtgc ggtctcgcca tccgctacgt ccttgtgccg tggggttttc
     7021 agaaatacac cggtgtggtc aacgcggagc tttcctatcc gaacagcaaa gcagtttcgg
     7081 aggcggagtt cgaggcaatc gcggcggatc ttcgttccga aggcattctc cggcgcgcgt
     7141 tggcgctcac gtcgtcggta acggattcga ttacgcggaa aacccggatc aggatgatcg
     7201 ccgccacacc gagtttccaa cggcaggaag aattcgcgca cattcgttac ttcagggatg
     7261 cgggtatccg gcgttatgag ggccccaaaa cgtgcatacg ttgccacgaa acgatagatg
     7321 tccggaagcc ggacggaacg attcagaagg tgaaaacgat ccccgacgtt ttgaacacca
     7381 tccattacaa gtttcagagc atggacggag gctttacgac gtacggttac gacggccggc
     7441 aggtcaacac aggcacccgc ggtatcccgg tcgggaagat agaccgcgcg tgcggagtcc
     7501 ccggcagttt ctcgtggaca ggctgggctg cgctgattga aacgaagccg gctcacgccg
     7561 gcggggaggt ggtccttcgc agcgaaggtt gcggtcagtg ccacatcgga ggcgggtacc
     7621 atcctgcaac cgaaaacatg atgcctggac tcgtcgtacc gacgccgaag gtcgcggcag
     7681 aagggctcga ttgcctgatc tgccatgcat ctgcctatga catgaatcag cgctttgtga
     7741 tcagagatga acacggtctc cggtggaatc aggacaggtc actgcgggcc gcgctctccg
     7801 tcggcagacc gacaaacgaa aactgcctga attgccacca gcacaacatg ggtggagaca
     7861 cgtacgccta caacacctcg gcacagtcgc tgggcaccca gaatcagcga cttctgcaca
     7921 gcactgccaa gcgtggcaat ccgttcagcg tgcatgatga tgtccacgcg cgcgccgggc
     7981 ttgtgtgtct ggattgccac cgaccgcagg ggcataagat tccccgggga aacaagggaa
     8041 cggatctggt ggcgaacgac ctcccggatg tcaaggttga atgcgagatg tgtcacacgt
     8101 ccgcacccca tgtccggaat cgcaggactc gtgcggcgtt gaatggtcac gcagattaca
     8161 tcgcctgtga aacctgccac atcgctcgcc tccttccctt caacaacgtg ttgaaagact
     8221 gggtgcatcc ggtctggaac gaggaagaag gcatgtacgt tccgaaggcg gtgtacagca
     8281 gcggcgatcc caaccggggt ctcgcgtatc tgtggttcaa cgggaacgga acgtttctcg
     8341 cgaatgctct cggcgccaac cccaaccaca gccccgatta caacccgctg atgcgccaga
     8401 tagttatgat agaagacccg gtggttctcg gagaaatcgc cgcgaacacg cgggatatcc
     8461 gaacgcgcta cgggctcgat tcagcttcgt acatggcgcc tatcgccaat gcgctatcgc
     8521 aactctcgcc ggaaatgctt tcgcggcggc gggagatgat tgaacgcaat ctgcgcgtcc
     8581 gtatgaatga agggaagagc agaatctatc ccttcaagct tttcaacgcg atgatgtttg
     8641 aggacctgaa caacgagggc ccgttcggtg caatgattct gccgttcgac taccggacgt
     8701 atttcgaaac aggggacgcg gagaacgccg tcaaggttgc ggtcgcgaat cccatcgtga
     8761 aacggatgta tgagaccccg ttcaagctct acatgatgga cgaattcatg gcgtatttcg
     8821 gggttgggaa gtggactgcg aaatacccgt tggacgaggg caactggaac gttcaacctc
     8881 gctggatgcg ccagatgggg actctcatgg tgaaccacgg catccagccc gtaggcaggc
     8941 agtgcgccga gtgccacaac cggaacggta ttctggactt cgcggcgctc ggatacacaa
     9001 cggaccgagt gcgcgcactt cagaacctgc cggaattgag ctattttcag ccaccgttac
     9061 gcccgtccca ccggcaggaa ggcgcagagg ttcaggcaga ggcaacagat gcttcagaac
     9121 gataagatct gggtaggcaa gggcgcgaat cccgtatacc tgctgccgac catggcaaat
     9181 cggcacgggc tcattgcggg ggcaaccggc acggggaaaa ccgttaccct gaaggtggtt
     9241 gcagaagcgt tcagcgacat gggcgttccg gtgttcgccg ccgacatcaa gggtgatctg
     9301 gcagggatag ccgttgccgg aacacctaac cccaaggtgg aagaacggtt gcgcaaactc
     9361 ggcgtcgaag gctttcagta cgcgggcttc cccgtccgtt tctgggacct gtttggcgaa
     9421 gcggggcacc ctgtgcgcgc aacggtctcc gacatgggtg cgcttctgct ttcgagaatg
     9481 cttgggctga acgatacgca ggcgggagtg ctgaacatca cctttcgggt ggcggatgac
     9541 aacggccttc tccttctcga tctcgcggat ctgcgcgcaa tgttgcagta cgtcggtgac
     9601 cacgcgagcg agcttacgac cagatacggc aatgtttcca ttcagagtgt gggagcgatc
     9661 ctgcgtgctc tgttgacact ggaagaccag gggggcgcga agttcttcgg tgagccggcg
     9721 ttggacattt ctgactggat gcaggtggac gcgcagggca aggggtacat caacatattg
     9781 cacagcgtga agttgtacat gtcccccgcg ctctattcca cgttccttct gtggatgttg
     9841 tcagagctgt tcgaagtgtt acctgaggcc ggagacctgc agaagcccaa aatggtcttc
     9901 ttcttcgacg aagcacatct gttgttcaaa gacgcgccca aggcacttct ggagaaaatc
     9961 cagcaggttg tccgtctgat tcggtccaaa ggcgtcgggg tctacttcgt tacccaggtt
    10021 ccctctgacg ttccacaaga ggtccttggt cagcttggca accgcgtgca gcacgcacta
    10081 cgtgcattca cgccggctga ccaaaagttt gtgaaggctg ccgcaagaag ctttcgcgcc
    10141 aacccgagtc tggattccga gaaaacgatc ctggaacttg gcacaggcga agcgctggtt
    10201 tccttcctcg acgcagatgg aaagccttcg attgccgaac gtgcgatgat cctgccaccg
    10261 cagagttcct tctccgctat cgacgacagt acccgcagcc gaattatctc cgcttcgctg
    10321 ttgggccgca agtatgagac cgcggtgaac aggaaatcag cattcgaaac gttgcaggcg
    10381 gagtcgcagg cgccgcgtga ggcgccacga gaacggacga ctacgccgaa acgagtgccc
    10441 accggaacca ccggacggag aaccagaacc ccgctggaag ccgccgcggg ttcgatgatg
    10501 acgacgatcg gccgggaggt gggcagacag attatccggg gcatcctcgg gtcgatactc
    10561 aaacggtaac tcgcggggcg ttttcgaaaa tcgcagcgcc gggcgactgg cgggttcaat
    10621 cagccgcccc ggccccctcc agaaacacaa gaatggcggt tgcatgctga ccaagagaat
    10681 cattccctgc ctcgacgtgc gagcgggaaa agtcaccaaa ggggtcgcct tccaggggaa
    10741 catcgacgtt ggagaccccg tagccatggc aaagcattac tacgaacagg gcgcggacga
    10801 gatcgtcttt tacgacatca ctgcttccaa cgagcgacgg gatatcatga tcgatgtcgt
    10861 ggccagcgta gcacgagaga tcttcatccc gttcagcgtg ggcggtggac tgcgaacgct
    10921 ctccgacatg cgacgggtgc tccttgccgg ggcggaaaag gtcagtatcg attcgggagc
    10981 cgtcaggaac ccggacacga tacgccaggg ggcggaagcc ttcgggcggc agtgtatcgt
    11041 gctatccatg caggtgaaaa gggtcccgca gacgccgacc attccctcgg ggtatgagat
    11101 ttacattgat ggtgcgcgca cagccaccgg gcttgacgcg atcgaatggg cacaacgcgg
    11161 cgaggagctt ggtgcggggg aactcgtggt gaactcgatc gacgccgacg gcgtcaaaac
    11221 cggctacgat gttgacctga ccagacgcat ggcggaagcc gtgggcatcc cggtgatcgc
    11281 ctcaggcggc gcgggagagc cttcacacct gtatgacgtg ttctctcaag gaaatgctga
    11341 cgcggccatt gtcgcctcaa tgatccattt cggcacgtac tccattccgg aaatcaagga
    11401 gtatttgcac cggcgcggcc tcccgatcag gatggtgtga gcccctgagc aatactggga
    11461 gagaaaggcg aaagcgggct ttttctccgt tggatggcac atcatcaaag cacgcgcagg
    11521 tggaacccgc cgtctaccac aatcgtgatg ccggtggaat acgaaaatcg gccctctgcg
    11581 attgcgacaa ccgcgtgcgc cacatcctcg ggttgtcccc accttttgat gggcagaagg
    11641 tttccttcaa gaatcagttt gtcgtaccgt tcccgtactt tttccgtcat atcggtctga
    11701 attacccccg gctgaacctc gtacacaccg atgccatccc cggcaagccg cgccgcgaac
    11761 actttggctg ccatcgcaag gcccgccttg ctgatgcagt agtcaccgcg attcacggag
    11821 ggtgcaaagg cgctgaccga ggttatgaac acgattttcg gctcacgaac gagccccgcg
    11881 cgctgccatt cgatcatttt cgtcgccaca atctgagtga ggaaataggg acccttcaga
    11941 ttcgtgcgaa caagcctgtc aaagctctct tcagtcgact ccagaacatc ggcgcggact
    12001 tccggcgcaa tacccgcgtt gttgacgagc agcgtcaccg gaccgagtcg gttttccgtt
    12061 tcggcaatca gctgtgcccg gtcatccgca tcagcgatat ccgcgcggac cggcatggcg
    12121 tgccgcccga ggcggctgat ttcctcacag gtttccgccg cggcttccgc gttgtgcgcg
    12181 aagttgaccg ctacgtcgaa attcgccttc gccagcccga gcgcaattcc tcttccgatc
    12241 ccacgcccgg ccccggtcac aacggctacg ggaggttttg ttgccatgcg aactcctttc
    12301 acgcagatcg tccggcatca ccagcaggga ggtcccgaaa cgcccagtct gtggaaggcg
    12361 ctcctggcgg cggcgttgac caccagaacc aacttctgca acgtagtcaa ctgaaccttc
    12421 ccgaccagca cggaatgagt gttccggctt atctggagcg ctacttcgcg atacacgctc
    12481 cccatcaagg caacagccat ccgggaatcg ggagagaccg caccgcaaag ggggcgggcc
    12541 agatcgaagt agcggaacac gcgctcgagt tcgaattcca tcagttgcag aaacccgggc
    12601 gcaggctccc gtgatttcag gtcctcctcc ctgcacccga accgaaccag gtcctcctgc
    12661 ggcacgtaga ttctcccccg gcgtgcatct tccgcaacgt ctcgaaggat attcgtcatc
    12721 tgcaaggcga tgctcagttc cgtcccccag cggggagtcc gcgggtccgt ccagccgaaa
    12781 atcgccagcg acagttcact gatggtggtg gcgacgcggt cgcagtacac cagcaactcc
    12841 tcgaacgttg cgtaccgcgt tttgacaaag tccatccggc acccttcaat gagccgcctg
    12901 aacgattcga ggggaatggg aaaacgagct gccgcatctg ccagtgccac caggatcggg
    12961 tgatcggtaa ctccctgata acaccgttcc agcgcttttt cccacgcgtc cagcagggct
    13021 tcggggttct cgtgtccggt gtactcgtcg gcaatgtcgt ccgcaaacct gcagaacgcg
    13081 tacacggcgt acaccgcgtc gcgtttgcgt ttcggaaggc aacggaaacc aacggagaag
    13141 ttggctccgt acaggtgcgt gatttcccga caatagcggt agcacccggc aagatcgtcc
    13201 aatcggggaa tcgaaaatgg cgaagacatg atcagacatt acccctttgg caatctgcac
    13261 cagatgcacc gagcgccgtg cacacattcg ggccgtcttt tccacaccgc ccgggcaatc
    13321 gtcgccaggg cgcgtttggc ggcggtgggc ttgtagaggg cctgacccac ctcacggaaa
    13381 tccgaacggg aagctttcgc aaggaaccac cgatacgcga catgcccgcc ggtttcgtag
    13441 agaaaacggt tcgccagccc catttcgaaa cgctgcccga aatgctcctc ccacgccggc
    13501 ccgaaagccc gtcctgaaag cacacactgt gcggccagaa caccagattg cagggcaagc
    13561 ctgatcccga acccgaaaag atggtctgc
//
LOCUS       DAOTNG010000047        13349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4498_length_13349_cov_4.693621, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000047 DAOTNG010000000
VERSION     DAOTNG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4498_length_13349_cov_4.693621"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="P5179_03655"
     CDS             complement(<1..307)
                     /locus_tag="P5179_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRS94348.1"
                     /translation="MGQSPVRVRFAPSPTGYLHIGGARTALFNWLFARHEKGTFILRI
                     EDTDQTRVVEGAIEAIFAGLRWLGLEWDEGPDVGGPVGPYIQSCRLDIYRQHVDNLIA
                     "
     gene            complement(801..894)
                     /locus_tag="P5179_03660"
     tRNA            complement(801..894)
                     /locus_tag="P5179_03660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(857..859),aa:Ser,seq:gct)
     gene            1050..3062
                     /locus_tag="P5179_03665"
     CDS             1050..3062
                     /locus_tag="P5179_03665"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS94349.1"
                     /translation="MKNSDGPIIGAICLNPGNDQAIETLRHIASLSMTMCETILVRGD
                     TAPARVLEIRRFLDEAQKLGVRVLLEPTYEPDLSRHPGMRNIGADGKISTRDNGFCYR
                     NKDYLAELSVFVAEVAQLYGDHPGILRVGGRPVIAIGHEMQYQITPQDGHGGAVRTMT
                     CYCGYCLHGFREWLRSLYGTISAFNRVHSRQVLSFGRVTPPRKPEPDCRLWQEWVDYH
                     ATAIPECIALQRQIIERELRGALVTHEINDWYPNTWDCVYTGNHFWRMGEKLEHSFND
                     QYPMEWAPGSLWRIYLYTFTQDVNQSAMGFERSFWTNGQAFPSWQGDFTEPPDAGYHE
                     QVYSALIHGANGLIWWWGGDYLRRSEAASREYAKLVRIVGNTRPAKDPVAILAPWTTF
                     AQTRSSDRGDDLMGGYQLLIRLGFQVEVVDERQVSEGVLASRGHRALITWGNSCVSPE
                     ARKEIEDFATRGGLVLADFGDQNVAPFGTIFPRTISRNPTRARAYVLPDGTTVAVRSG
                     AQALAAGKRVEVIARFDDGRPAIVRQPIGSGVLWRAGSFVGLDYAEGMGVYDWVQQQR
                     VRIEPALENLIAEALERQGLRPIGSTNNRNAEVGIFPVDSRLLVLVANHLRHPLQVTV
                     RVRITGWASAKDVFSGEIAECIQEGDATAFSVRLPEMGGRAFWLMR"
     gene            complement(3066..4274)
                     /locus_tag="P5179_03670"
     CDS             complement(3066..4274)
                     /locus_tag="P5179_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRS94350.1"
                     /translation="MSCSVVNLTKALVAIPSESSRSNLPVMQQLCTFLYSQGFRVTRY
                     PQNGPSTGPEKTNLIAERGNGTRRLVFSGHLDTVPVGERGQWQHDPYGAEGIIGDRLY
                     GRGTVDMKGQVAAMACACASVPEEVLRNLTLTLAITGDEEIGHVGIKDLAAKHVFERA
                     VGCVVGEPTGLRVVSAHKGGMTVRITVRGLSCHSSKPHLGVNAIEQAVRLLNLLGERL
                     APWCAQRHPAFGEEPPTFTVVGVSGGVADNVVPDSCTVNISARALETGHFETFRAALE
                     GIISELVDDDTRKGVPPERRFSADMDLLKWAPPMVCDTAGVWYRTVARIAGQEKPEFV
                     TYGTDAGVLNQVGLPCVVWGPGDIIRAHSVDEYITLEELTTAVTRYRELICAVAASEL
                     PTFTTSLWNG"
     gene            complement(4294..4995)
                     /locus_tag="P5179_03675"
     CDS             complement(4294..4995)
                     /locus_tag="P5179_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94351.1"
                     /translation="MQNPPPQEHRAASLSARLLLRDTVRAYLRFLPVFVAVMALRLVP
                     FYVLFWFLPPGTDAVHAVMETAVAVPCVAALVRIVWNGSDAARPRFADSLLGASPVAT
                     LKLLVTNLFLLTVALMVAQISPEWFLGFVIVWTWAALLTDQVVLIEGKMMITAIARSY
                     GIVRRAWKLSLLGLLAVLLPEALNLTITEFVPEALKKELLTRGVTVLFLPFSTTVLTL
                     LYERTESLAAPPFDG"
     gene            complement(5083..5652)
                     /locus_tag="P5179_03680"
     CDS             complement(5083..5652)
                     /locus_tag="P5179_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS94352.1"
                     /translation="MRNGLLSAFLVVGLACDAMAQGGYIPVIYAANKEYGLSGSYLKQ
                     KDAADNGTSDLTLMGHWGIMMPLGLQPELEAGWRKSTTDVPDFQARDYSLAGNLLWNL
                     SPFNSLSFFGLAGYGYIGAKTETKVGTVTTTLTDNRWFSQFGGGVKWLLVPNAGIRLD
                     YRYQNGATLNAAGNKQSRHVLLLGVSVFQ"
     gene            complement(5685..7175)
                     /locus_tag="P5179_03685"
     CDS             complement(5685..7175)
                     /locus_tag="P5179_03685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRS94353.1"
                     /translation="MAAKNTVLVAHHDSATRDLFREALDKEVFDTIITDKGTEARELF
                     ETLGDRLDAVVLDSQLPDVDPYELLAEFRKGTSDAEIVVVVNEVNEDAFSRALGAGAF
                     DFLVQPFSKLAAQQMLRNAVERRRCRRLTADLVSTKRSLEVELTVKSFQIDQVRELVN
                     FSRLVNEHLRLDAILDITFERLPELVNAETFSLFLYEPSRNEFHLAITNRPNLDPREP
                     IVVKEENSPIMKRAIETHQVLFIDDVLKSGLLPDEQARTAKSRHYKKKHSVCLPLKVE
                     DRPIGVLNLNDFRAEEQIQDYINVAVLAAAFLAPVISNARLYEQVEETANRDGLTQLY
                     NRAFFDKNLGENFRRAARYGRPLSLLFLDIDHFKQLNDTYGHQAGDMVLRRVAARLLD
                     NSRKGIDVAARYGGEEMVLIGPETPADGIKIVAERIRQDIEAMRVKFRNESGEKTLSV
                     TVSIGTATYLPGVSDYPSERALTEAADSAMYQAKHSGRNRCSHHNG"
     gene            7415..8716
                     /locus_tag="P5179_03690"
     CDS             7415..8716
                     /locus_tag="P5179_03690"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRS94354.1"
                     /translation="MAGSVQVGLVGLGTIGVGVARLLLEKRSKVFAGDCGPITLKRIA
                     DKDLSDRGIDLPSGILTEDVRAVLEDPEIGIIVELVGGIEPARTFVLTAIEHGKHVVT
                     ANKALLSAHGEEIFRAAQKRGVDVLFEAAVGGSIPIIRALRESLGTSAIRSIYGILNG
                     TTNYILTRMTDDGASYDEMLKAAQQLGFAERNPVADVSGKDSLQKLVILARLAFGASL
                     SPEEILCEGIERITPMDISFAQELGYRIKLLAIAKEREGRIEARVHPAMIPLSGLMAN
                     VKFEYNAIEVVGEEFGTQVFYGKGAGQRPTATVVVSDVIDLALRIQSGAPTCRVAPLL
                     ASASSVRLVTAEDLFLRHYVRLEVFDRAGVLADIARLFAEENISIESVIQKGRAASDT
                     VPLVIMTHEASEASIERALRRLSTLNVVRGPIQRIRVEELE"
     gene            8719..9840
                     /gene="thrC"
                     /locus_tag="P5179_03695"
     CDS             8719..9840
                     /gene="thrC"
                     /locus_tag="P5179_03695"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRS94355.1"
                     /translation="MEPDVQNPRSAAWPGIIQRYRPHLPVTAGTPVITLLEGNTPLIP
                     AVALPRLFGADIELYFKFEGMNPTGSFKDRGMTLAISKAAEDGAEIVVCASTGNTSAS
                     AAAYASRAGMISTVLIPRGNIAQGKLAQAMMHGAVVIQIDGNFDDCLRLVRALAEKRP
                     VAIVNSINPYRIEGQKTGSFEICEQLGGRPPDYHVLPVGNAGNITAYWKGYREFFDNG
                     FIDRLPKMIGFQAAGSAPIVRGSRIEKPETIATAIKIGDPASWKSAVAARDESGGLID
                     EVTDEEITHAQKLLARYEGVFAEPASAASVAGVAKLSRKGFFPKGARVVCILTGHGLK
                     DPERAIAIAEAPVRSSANIDAVLDLLDRAVEARRRHSNP"
     gene            9859..11091
                     /locus_tag="P5179_03700"
     CDS             9859..11091
                     /locus_tag="P5179_03700"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRS94356.1"
                     /translation="MGLIVMKFGGTSVATPELITRVARRVLATKKEGNDVVVVVSAMG
                     DATDELESMAAKISVEPAEREMAILMITGERVSAALLAIALNELGQPARAFTGWQAGI
                     VTDSASPLRAKIRRIEATRIREALREGSVAVVTGYQGVTEDGDETALGRGGSDLSAVA
                     LACALKADACEIYTDVDGVYTTNPRLVKDARKIRAISYEEMLELASMGAQVMQARSIE
                     YAKKHGTVIHVRSSFSEAPGTVIREADTAMEAVVVRGIAYDMTEAKITVRGVTDRPGV
                     AATLFNRMAQAGINVDMIVQNVSEDGITDLSFTVTRPDLKKAMDACLLIQRDIGAREI
                     VADEEIAKISLVGIGMRSHTGVAAKMFDTLACHGINIEMISTSEIKISCIVREAQAET
                     AVKALHTTFALDREGDEY"
     gene            11117..11188
                     /locus_tag="P5179_03705"
     tRNA            11117..11188
                     /locus_tag="P5179_03705"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11149..11151,aa:Lys,seq:ctt)
     gene            11362..12210
                     /locus_tag="P5179_03710"
     CDS             11362..12210
                     /locus_tag="P5179_03710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS94357.1"
                     /translation="METRTLGRTGTQLSIIGFGGIVVAGKTQAEADRLVSFALDSGVT
                     YFDVAPTYGDSEKRLGPALQRKREGIFLACKTTERTREGAQRELERSLRRLRTDHFDV
                     YQLHGVSSMQEVETIFAPGGAIETIERAKTEGKTRLAGFSAHSEEAALALLNRYQFDT
                     VMFPVNWVCWSNAGLGARLVHTAKERGMGILAIKAMARTKWPENGRRDEYSACWYQPE
                     DDPATASLALRFAWSEGVTACIPPGDAEMLRLAIRVADNTAPLSEDERAYLTRKAQSY
                     IPIFPQ"
     gene            complement(12306..13220)
                     /locus_tag="P5179_03715"
     CDS             complement(12306..13220)
                     /locus_tag="P5179_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94358.1"
                     /translation="MNFSAVSFAYLVDSEETEEQAAVLSRSIRDLGGGLCASQIIAMR
                     PETSRPSRRWNALKDDLQVIESVFAMEAELSTLPFATKAVAARHAESRVAESGDLLVW
                     MDSGSLVLREPAELLLDDDHVLGCSPVDHTLIGSPFSEPLDGFWEYVYRACGVDRSSV
                     FPVTSVVDRRKIRAYFNAGLLVVKPQRGILRAWGENVRRICTGADVKGLLKGNPLHRV
                     FLHQAVLAATILARVDRNEILLFTPHVNYPLHMHADHPANQATKSMDDLVTCRYEGFF
                     ARDDWAKTVPVSDALEAWIRTHLCRADS"
BASE COUNT         2878 a         3781 c         3903 g         2787 t
ORIGIN      
        1 tcgcgataag gttgtcgacg tgttgccggt atatatcaag cctgcacgac tgaatgtacg
       61 gcccaaccgg ccctccgaca tccggcccct cgtcccactc caatcccagc cagcgaaggc
      121 cggcgaaaat ggcttcaatt gcgccctcaa ccactcgcgt ctggtccgtg tcctcgattc
      181 ggaggatgaa ggtccccttc tcgtgtctcg caaaaagcca gttgaaaagg gctgtgcggg
      241 ccccgccgat gtgcaggtat cccgtcggac ttggcgcaaa gcgaacgcgt accggcgatt
      301 gtcccatatt cacgctttca attcacaagg tttgcttgcg cggcctctct tcgaacgtcc
      361 ttgcggcaaa agaggggctg cctaacccgc aggaatcggt acggacggcg gaggattcct
      421 gatttggcgc agtgtccttc tcagaactct ctgcggtgaa aactgtgttc gcacccaacc
      481 gcactgagag attgtacagc gccgctccgg cagcaaacgc cgagcaccaa aggctcccgt
      541 tcgcgagggt gatcagtacc accccggaaa cgccaatccc gttcacaggc tggtcgagga
      601 cctgcccaag gggggcgagc gctgatccga tcaaaacaaa tgctatgccg aaaagcagtc
      661 ctgacaggca cccgagcact gcgcccactt tggccgccgg ccacacgtga acacgcgtta
      721 tcaccaggcg catcagtcga accctcagac gtcaatgcct ctccaccgat atctgggtgg
      781 agaggcacgc gcaggaaata tggcggagag ggcgggattt gaacccgcgg tggggctttt
      841 gaccccacac tcgcttagca ggcgagcacc atcggccagc tcggtcacct ctccgttcag
      901 ccggaagtca gacaatgaag ttacggcacg cacgaggaat gtgtcaagac gaggaagagt
      961 ccttggttgg tgtcacgccg tcgcatgcct attgtactga cagatccttg caatgactta
     1021 ataacacgct gccccaccgg agacatcgga tgaagaactc ggacggtcca atcatcggcg
     1081 ctatctgcct caatcccggc aacgaccaag caattgagac actgcgccac atcgcgtctc
     1141 tttcaatgac aatgtgcgaa acgatcctcg ttcgagggga taccgcgcct gcgcgcgtgc
     1201 ttgagatacg ccgattcctc gatgaggcgc aaaaactggg tgtgagagtc ctcctcgaac
     1261 cgacgtacga accagacctc tcccggcatc cagggatgcg gaacattggt gccgacggga
     1321 aaatcagcac acgcgacaac ggattttgtt accgcaacaa ggactacctc gcagagctct
     1381 cggtctttgt ggccgaggtc gcgcagttgt atggcgacca ccccggtatc cttcgggtcg
     1441 ggggccgccc ggtcatcgcg atcggacatg agatgcagta tcagataacg cctcaagacg
     1501 gacacggagg cgcggttcgt acgatgacat gttactgcgg atattgtctc cacggcttcc
     1561 gggaatggtt gcgcagtttg tacggaacca tttctgcgtt caacagagtc cacagccgcc
     1621 aggtgctttc ttttggccgc gtgacgccac ctcgaaaacc ggaacccgat tgccgccttt
     1681 ggcaggaatg ggtggactac cacgcaacgg cgattccgga atgcattgcg ctgcaacggc
     1741 aaatcatcga acgagaactt cgtggtgccc ttgtgaccca cgagatcaat gactggtacc
     1801 caaatacgtg ggactgcgtg tacaccggga accatttctg gaggatgggc gaaaagctcg
     1861 aacattcttt taacgaccag taccccatgg aatgggcgcc tggatcactt tggcggatct
     1921 acctgtacac gtttactcag gacgtcaacc agagcgcgat gggctttgaa cgctcgttct
     1981 ggacgaatgg gcaagcattt ccttcgtggc agggggattt caccgaacca cccgatgcgg
     2041 gctaccacga acaggtgtac agcgcactta tccatggtgc gaacggcctg atctggtggt
     2101 ggggagggga ttatctgcgg cgttcagaag cggcaagccg tgaatacgcg aagttggtcc
     2161 ggatagtggg aaacacgcgg cctgcaaagg atcccgttgc gatccttgcg ccatggacca
     2221 cgttcgcaca gacgcgatca tccgaccgtg gtgacgatct gatgggcggg tatcaacttc
     2281 tcatcagact cggattccag gtggaagttg tggacgagcg gcaggtttca gaaggtgttc
     2341 tcgcatcgcg cggtcacagg gcgctcataa cctggggcaa ctcatgcgtt tcgccagagg
     2401 cgcggaaaga aatcgaagat ttcgctacgc gcggagggct tgttcttgcc gacttcgggg
     2461 atcagaacgt tgcgcctttt ggcaccatct ttccgaggac gatttcccgg aatccaacgc
     2521 gggcgcgggc gtacgtactg ccggacggaa cgacggtggc cgtgcgctcg ggagcacagg
     2581 ctctcgccgc cgggaaacgt gtggaagtca tcgcgcgttt cgacgacggg cgcccggcga
     2641 tcgtgcgaca gccgatcgga agcggcgtgc tgtggcgcgc tggttcgttc gtcgggttgg
     2701 attatgcgga aggaatgggc gtgtacgatt gggtccagca acagcgcgta agaattgaac
     2761 ctgccctgga gaatctgata gcggaagcgc tcgaacgaca gggcttgcga ccgatcgggt
     2821 caacgaacaa ccgaaacgcg gaggtgggga tcttcccggt cgactcgcgg cttttggttc
     2881 tcgtcgcaaa ccacctccgc catccgttac aggtcactgt gcgcgtgcgt attacggggt
     2941 gggcctcggc aaaggatgtc ttctcaggtg aaatcgccga atgcattcag gagggggatg
     3001 ccaccgcttt ttcggtgcgg ctgccggaga tgggaggacg ggccttctgg ttgatgcgct
     3061 gaacctcacc cgttccagag cgacgtggtg aaagtcggta gctcggaggc agcaaccgca
     3121 cagatgagtt cgcggtaacg ggtaacagcg gttgtaagct cttccagagt gatgtactca
     3181 tcaacggaat gagcgcggat gatgtcaccc ggtccccata cgacgcatgg aaggccgacc
     3241 tggttcaaca ctcccgcgtc agttccgtag gtgacaaatt ccggcttctc ctgtccggcg
     3301 atccgtgcaa ctgtccggta ccacacgcct gcagtatcgc acaccatcgg aggggcccac
     3361 ttcagcaggt ccatgtccgc tgagaaccgc cgttccggag gaactccctt tcgtgtgtca
     3421 tcgtcaacaa gctcgctgat gatcccttcc agtgcggctc ggaatgtttc aaagtgcccc
     3481 gtttcgagtg ctcgggcgct aatattgaca gtgcatgaat caggtacgac gttatccgca
     3541 acgccgcccg agacgcccac gaccgtgaag gtgggcggtt cttctccaaa agccgggtga
     3601 cgttgcgcgc accagggtgc gaggcgttct ccgagaagat tcagtaaccg gacagcctgt
     3661 tctatggcgt tgacaccgag gtgcggtttg cttgaatggc aactaagacc tcgaacggta
     3721 attcggacag tcatcccgcc cttgtgcgca ctcaccacgc gcagaccggt aggctcaccc
     3781 accacgcacc ccacggcgcg ctcaaaaaca tgcttggcag cgaggtcctt gatccctacg
     3841 tgaccgattt cctcgtcccc tgttattgcc agcgtcaagg tcagattcct caacacctct
     3901 tccgggacag atgcacacgc gcacgccatc gccgccacct gccccttcat gtcaaccgtt
     3961 ccgcgaccgt agagacggtc accaattatc ccttctgcac cgtaaggatc atgctgccac
     4021 tgcccgcgct ctccaacggg gaccgtatcg aggtgaccgg aaaacacaag gcgacgagtt
     4081 ccgtttcctc gttctgcaat caggttggtt ttttctggtc cggttgacgg accgttctgc
     4141 gggtatctgg tgacgcggaa gccttgcgaa tacaggaacg tacagagttg ctgcatgacg
     4201 gggaggttgg atcgcgagct ttcgctcggg atcgcgacca gtgccttcgt caggttcacg
     4261 acggaacagc tcaagtcctt actatccttc ttgtcaaccg tcaaacggag gtgccgccag
     4321 cgactccgtg cgttcgtaca agagagtcaa cactgtcgtc gaaaacggga gaaaaaggac
     4381 ggtaactccg cgagttagaa gctccttttt cagcgcctcc ggaacgaact cggtgattgt
     4441 cagattgagc gcttcgggca gcagaaccgc caaaagcccc agaagagata gtttccacgc
     4501 acgtcgcaca atgccgtacg aacgtgcgat tgccgtgatc atcattttcc cttcgataag
     4561 cacgacctgg tccgtcagaa gtgccgccca tgtccacacg atgacgaatc caaggaacca
     4621 ttcggggctg atctgcgcca ccataagtgc gacggtgagc agaaaaaggt tcgtcacaag
     4681 caacttcaga gtcgcgactg gagacgcgcc gagaagggaa tcggcgaaac gaggacgtgc
     4741 tgcatcgctt ccgttccaca caatcctgac aagggccgcg acgcagggaa ccgccactgc
     4801 cgtttccatt accgcgtgta ccgcgtcggt gcccggagga agaaaccaga aaagaacgta
     4861 gaaagggaca aggcgcagcg ccataactgc tacgaagacg ggcagaaaac ggaggtatgc
     4921 gcggactgta tctcgaagaa gcagtcgagc tgaaagtgaa gccgcgcggt gctcttgcgg
     4981 cgggggattt tgcatttcgt tctactcgat cacagggaac aaacgcgcac gttgcgctac
     5041 tccgcggcaa ctttctactt gccggttggg caaaccagcc gtttactgga aaacggaaac
     5101 gccgagaagg agtacgtggc ggctctgctt attccccgcg gcattcagtg tggcaccgtt
     5161 ctggtagcgg taatccaacc gaatcccggc gttcggcacg agaagccact tgactcctcc
     5221 gccaaactgc gaaaaccacc ggttatccgt aagcgtcgtg gtgaccgtcc caactttggt
     5281 ctccgtcttc gcgcctatgt acccgtagcc agccagaccg aagaaagaaa gggaattgaa
     5341 tggactcaga ttccacagga ggtttcccgc cagcgaataa tcccgtgcct ggaaatccgg
     5401 aacgtctgtt gtggatttgc gccaccccgc ttccagttcc ggttgcaggc cgagaggcat
     5461 catgattccc cagtggccca tcagtgtcag gtcggatgtg ccgttatccg cggcgtcttt
     5521 ctgtttcagg tagctgccgc tcaatccata ctctttgttc gccgcgtaaa tgacagggat
     5581 gtacccgccc tgagccatgg cgtcacaggc caacccgacg acgagaaagg cactcagcaa
     5641 tccgttcctc attcgacacg ctcctttgtc catcgcggga cgtattaccc attatggtga
     5701 ctgcaacggt tacggcctga atgtttcgcc tggtacatgg cgctgtccgc cgcttccgta
     5761 agagcacgtt cgctcgggta atccgacaca cccggaagat aggtcgccgt cccgatactt
     5821 acggtgacgg acagcgtctt ttctccggat tcgttgcgga atttcacccg catcgcctct
     5881 atgtcctgcc tgatacgctc ggccacgatc ttgatgccat ccgccggcgt ttcagggcca
     5941 atcagaacca tttcctctcc accataacga gcagcaacgt cgatcccttt gcgcgaattg
     6001 tctaacaggc gagccgcaac gcggcgcagg accatgtccc cggcctgatg gccgtacgtg
     6061 tcgttaagct gcttgaaatg gtctatgtcg agaaacaaca acgagagggg tcgtccgtaa
     6121 cgagctgcac gcctgaagtt ttcgccgaga tttttgtcga agaaagcccg attgtacaac
     6181 tgcgtcaatc cgtcgcgatt cgccgtttcc tcgacctgtt cataaagccg ggcgttcgaa
     6241 atcaccggtg caagaaacgc cgctgccaga accgcaacat taatgtaatc ctgaatctgc
     6301 tcctcggcac ggaagtcgtt aagattcagc acaccgatcg ggcggtcttc gactttgagc
     6361 ggcaggcaga ctgagtgctt tttcttgtaa tgccggcttt tcgctgttct tgcctgttcg
     6421 tcgggcaaga gaccactctt caggacatcg tcaatgaaca gcacctggtg ggtctcaatt
     6481 gcgcgtttca tgatcgggct gttttcttct ttgacgacta taggttcgcg cgggtcgaga
     6541 ttggggcggt tggtaatcgc gagatggaat tcgtttcgcg agggttcgta caggaaaagg
     6601 ctgaatgttt cggcgttcac aagttcggga agtcgttcaa atgtgatgtc gagaatcgcg
     6661 tccaaccgca aatgctcgtt gacaagtctg ctgaagttta ccagttcgcg cacctggtcg
     6721 atttggaacg actttacggt gagttccacc tcaaggctgc gtttcgtact tacaagatcg
     6781 gcggtgagtc tcctgcatct ccggcgttcc actgcattcc gcaacatctg ttgcgctgcc
     6841 agtttgctga acggttgcac cagaaaatca aacgctcctg cgccgagcgc tctggaaaag
     6901 gcgtcttcgt taacttcgtt gacgacaacg acgatttcgg cgtcagaggt accttttcga
     6961 aactccgcca gcaactcgta cgggtcaaca tctggcaact gcgaatccag aacaaccgca
     7021 tccaaacggt cgccgagcgt ttcgaaaagc tcccttgcct cggtgccttt gtcagtgatg
     7081 atagtgtcga aaacctcctt gtcgagcgct tcccggaaca aatcccgcgt tgcgctgtca
     7141 tggtgagcta ccagcaccgt gtttttggcg gccatgcggt cggtctcgtt ctgttgcgtc
     7201 gtgcccggga gcaaaaaggc tctattggga attaagtttc gctcgttgat gatacgcgta
     7261 agtaattgaa ccggtcaaga gtatctcaat tgctacgggc tgcgatcctg cgggtttgcc
     7321 ccgccttgac cctccgcgga gcggagtggt acgttttaga acgtttttca cgttcttttt
     7381 gcgcttccgt ggtctatttc tagaaggcac ctcgatggcg ggtagtgttc aagtcggttt
     7441 ggtaggtctt ggaaccattg gagtcggcgt tgcgcgtctc ctgcttgaga aacggagcaa
     7501 ggtcttcgcc ggggattgcg ggcccatcac actgaagcgg attgctgaca aggacctttc
     7561 agatcggggt atcgatcttc cttccggcat tctgacggag gacgtaaggg ccgtgctgga
     7621 agaccccgaa atcggcatta ttgtcgaatt ggtgggagga attgaaccag cgcggacctt
     7681 tgtgcttacg gcaatcgaac atggcaaaca tgttgtgaca gcaaacaagg cccttctctc
     7741 tgcacatgga gaggagatat tccgcgcagc gcagaagcgg ggcgtggatg ttttgtttga
     7801 agcggcagtt ggcggcagca tcccgattat ccgggctctc agggaaagcc tcggtacgag
     7861 cgccatccgc tcaatttacg gcatcctcaa cggcaccacg aattacatcc tcacgaggat
     7921 gacagacgac ggggcatcgt acgatgaaat gctgaaagcc gctcagcaac tgggtttcgc
     7981 cgagcgcaat cctgtggccg atgtgtccgg gaaggattct ctgcagaagc ttgtcatcct
     8041 tgcgcggctt gctttcggcg cttctctttc gcccgaggaa atcctttgcg aaggtatcga
     8101 acgcattaca cccatggata tctccttcgc gcaggaactt gggtaccgaa tcaaactgct
     8161 cgccatcgcc aaagagcgtg agggccggat cgaggcccgc gttcatcctg ccatgatccc
     8221 cctttccggg ctgatggcga acgtcaagtt cgagtacaac gcgatcgagg tggttggtga
     8281 ggaattcgga acccaggtct tctacgggaa aggagccggg caaaggccga cagcgacggt
     8341 tgttgtttcg gacgtcattg atctggcttt gcgaatccag tcaggtgctc cgacgtgtcg
     8401 cgtcgcaccc cttctcgcgt ctgcctcgag cgtccgactc gtaaccgcgg aagatctctt
     8461 tttgaggcat tatgtccggc tggaggtttt cgatcgggcg ggcgtacttg ccgacattgc
     8521 gcgattgttc gcggaagaga acatcagcat cgaatcggtg atccagaagg gacgtgcagc
     8581 ttcggacacc gtgcctctgg taatcatgac acacgaagcc agtgaggctt ccattgaaag
     8641 ggcgctgcga cggctttcga cgctgaatgt cgtccgcggg cctattcagc gaattcgcgt
     8701 ggaagaactg gagtaacatt ggaacctgac gttcaaaatc cacgttcggc cgcgtggccc
     8761 ggcatcatcc agagatacag gccacatctg ccggtcaccg caggaacgcc cgtcatcacg
     8821 ctgcttgaag ggaacacgcc gctgatccct gcagtcgcct taccgcgact gtttggcgct
     8881 gacatcgaac tctacttcaa gttcgaggga atgaatccta ccggttcctt caaagaccgg
     8941 gggatgaccc ttgcaatctc gaaagcagcg gaggacggtg cggaaatcgt ggtctgcgct
     9001 tcgactggca acacaagcgc atccgcggcg gcgtacgcct cgcgggccgg aatgatctcc
     9061 acggtgctca taccgcgtgg gaatatcgct cagggcaaac tcgctcaggc aatgatgcac
     9121 ggcgcggtcg tcattcagat tgacggcaat ttcgacgatt gccttcggtt ggtaagagcc
     9181 ctcgcggaaa agcgccccgt tgcgatcgtg aactcgatca acccgtaccg aatagaaggg
     9241 cagaaaaccg gttctttcga aatctgtgag cagctcgggg ggcggcctcc tgactaccac
     9301 gttctgcctg tcgggaatgc gggtaatatc accgcgtact ggaaggggta ccgggagttt
     9361 ttcgacaacg gattcatcga tcgactcccg aagatgatcg ggtttcaggc ggcggggtcc
     9421 gcgcccatcg tccgtggatc gagaatcgaa aaacctgaaa ccatcgctac ggcaatcaaa
     9481 atcggtgacc ctgcgagctg gaaaagtgcc gttgcggcgc gcgatgagtc cggcggactg
     9541 atagacgaag tgaccgacga agaaatcacg catgcgcaga aattgctggc gagatacgag
     9601 ggggttttcg cggaaccggc atccgccgcg agcgtggcgg gcgttgccaa gttgtcgcgg
     9661 aaagggtttt ttccgaaagg cgcgcgtgtt gtgtgtatcc tgacagggca tggcctgaag
     9721 gaccctgaac gggcgattgc aatagccgag gcaccggtac ggtcatctgc gaacatagat
     9781 gctgtgttgg accttcttga ccgcgccgtg gaggcgcgga gacgtcactc caacccataa
     9841 caaagacgga aagctgctat gggattgatc gtaatgaagt tcggcggaac gtcggtagcg
     9901 acgccggaat tgatcactcg cgtcgcacgc cgggtcctcg cgacgaagaa agagggaaac
     9961 gacgtggtgg tggtcgtctc cgcgatgggg gatgcgaccg atgagctcga gagtatggcc
    10021 gcgaaaatat ccgtggagcc ggcagagcgg gaaatggcca tcctcatgat cacaggggaa
    10081 cgggtttccg ccgccctgct ggcgatcgcg ctcaacgaac tcgggcaacc ggcgcgcgcc
    10141 ttcacgggct ggcaggcggg aatcgtgaca gattcggcgt cacccctgcg cgcgaaaatt
    10201 cgtcgcatag aggctactcg tatccgcgag gccctgcggg aaggatccgt cgccgtcgtt
    10261 acggggtatc agggtgtgac ggaggatggc gacgagaccg ccctcggcag gggcggaagt
    10321 gacctctccg ccgtcgcgct ggcatgcgcg ctcaaagcgg atgcctgcga aatctacacc
    10381 gatgtagacg gcgtgtatac tacgaatccg cggctcgtga aagacgccag gaaaatccgc
    10441 gccatctcgt acgaagaaat gctcgaactt gccagcatgg gcgcccaggt catgcaggca
    10501 aggtcgattg aatatgcaaa aaagcacggg actgtaatcc acgttcgttc atcattcagc
    10561 gaagcgccgg gaacggttat tcgggaggca gataccgcta tggaagccgt agttgttcga
    10621 ggcatcgcgt acgacatgac ggaagcgaaa atcaccgtcc gaggtgtcac tgaccgtccg
    10681 ggcgtcgccg caacgctttt caatcgcatg gcacaggccg ggatcaacgt ggacatgatc
    10741 gtccagaacg tgtccgagga cggcatcacg gatctctcgt tcaccgtgac gcgtcctgat
    10801 ctgaaaaagg cgatggatgc gtgtctgttg atccaacgcg atatcggggc cagagaaatc
    10861 gttgcggacg aggagatagc aaagatttct ctcgttggta taggtatgcg ctcgcacact
    10921 ggtgtcgcgg cgaagatgtt cgacacactc gcctgccatg gaatcaatat cgagatgatc
    10981 agtacttccg agatcaagat atcgtgtata gtccgtgagg cgcaggcaga aactgccgta
    11041 aaagcgctac acacgacgtt tgctcttgac cgggaaggcg acgaatactg atatttaagt
    11101 tttgcagttt gcctttgcct ccatagctca atggtagagc agcggactct taatccgttg
    11161 gttgaaggtt caagtccttc tgggggtact ccttgaacgg cacaggtgtt tcggtctgcc
    11221 tgtgccgttt ttcgtttgtc tcccgtcctt gtgttctcaa cgccttattg cgcacgtttc
    11281 gtggcgtgac catattccat gacgactatc catcacgaga aatgacgagg agttgcagcg
    11341 cgggtctgga ggccggttca aatggaaacg agaacccttg gtagaactgg aacgcagctt
    11401 tcaatcatcg gattcggggg aatagttgtc gccggcaaga cacaggctga agcggacaga
    11461 ttggtcagct tcgcgttgga ttcgggcgta acgtatttcg acgtcgcccc gacctacggg
    11521 gattcagaaa aacgcctggg acctgccttg cagcgcaaac gcgaaggcat cttcctcgcc
    11581 tgcaaaacga cagaacgcac ccgagaagga gctcaacgcg aactcgaacg ttcgcttcgg
    11641 cgcctgcgca cagaccattt cgacgtgtac cagttgcatg gggtgtcaag tatgcaggag
    11701 gtggagacca tttttgcccc cggtggagcg atcgagacta ttgagcgtgc gaagacggaa
    11761 ggcaaaaccc gcttggctgg tttttccgcg cactccgaag aagccgcact tgcgcttttg
    11821 aaccggtacc agtttgacac ggtgatgttc cccgtaaact gggtctgctg gtcgaacgcg
    11881 ggtttaggcg ctcgccttgt tcacacggcg aaagaacgcg ggatggggat tctcgcgatc
    11941 aaagcgatgg ctcgcacaaa atggccggag aacgggcgcc gagacgagta ctccgcgtgc
    12001 tggtatcagc cggaagacga cccggcgacg gcgagcctcg cgctgcgttt cgcctggtct
    12061 gagggcgtca ccgcgtgcat tccgccgggc gatgccgaaa tgctccggtt ggcaatacgg
    12121 gtcgcggaca acaccgcgcc tctttccgaa gacgagaggg catacctgac gagaaaggcg
    12181 caatcgtaca tcccgatttt tccccagtaa gagcgttcgc gcaagaggtc ggtacgtgag
    12241 atgaaagcgg ctgctccgtg catgtttgtg cggctgcaac ccctccccat gactcccgga
    12301 tggcgtcagg aatcggcccg acagagatgc gttctgatcc aggcctccaa cgcgtcgctc
    12361 accgggacag tcttggccca gtcgtcacgt gcgaagaacc cttcgtagcg gcacgtaaca
    12421 agatcgtcca tggatttcgt tgcttgattg gccggatggt ccgcgtgcat gtggagaggg
    12481 tagttcacat gaggagtgaa caacaggatc tcgttccgat ccactcgcgc aagaatcgtc
    12541 gcggcaagta ccgcctggtg gagaaagacc cggtgcaacg ggttaccttt gagcaaccct
    12601 ttgacgtccg cacccgtgca aatccttcgc acgttttctc cccacgcacg aaggattccg
    12661 cgctgcggtt tgaccacaag aagtcccgcg ttgaagtagg cgcggatctt acgccggtcc
    12721 acgacgcttg taaccgggaa caccgaactg cgatccaccc cacaagcccg gtacacgtac
    12781 tcccagaacc catccagcgg ctccgaaaac ggtgacccga tcagcgtatg gtcgactggc
    12841 gagcaaccca gcacgtggtc atcgtccagc aacaactccg ccggctctct caggacaagg
    12901 gagccgctgt ccatccacac cagtagatcg ccactttctg ccacccgcga ttccgcatgt
    12961 cgcgccgcga ccgccttcgt ggcgaatggt agggtggaca actccgcttc catcgcgaac
    13021 acgctctcga ttacctgcaa atcgtctttg agagcgttcc acctgcggga aggacgcgat
    13081 gtctccggcc tcatcgcgat tatctggctc gcacaaaggc ccccgccgag atcgcgaatg
    13141 ctgcgtgaga gcactgccgc ttgttcttcc gtctcctcgg aatccaccag atatgcgaag
    13201 gatactgcgg agaagttcat ttgcagtgtt gagttcctgt ggctgacgca ggctcagccg
    13261 cctgtcggta ccgatcggta cgcctgaaaa cggacacgac cagttctggt tcaatcgcaa
    13321 ttcgcgtggg ccggggcccg cggcttttg
//
LOCUS       DAOTNG010000048        13038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4613_length_13038_cov_5.490102, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000048 DAOTNG010000000
VERSION     DAOTNG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_13038_cov_5.490102"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="rpsB"
                     /locus_tag="P5179_03720"
     CDS             complement(<1..738)
                     /gene="rpsB"
                     /locus_tag="P5179_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRS94359.1"
                     /translation="MARVEMKDLLEAGVHFGHQTRRWNPKMKRFIFMERNGIHILDLN
                     KTIALLENAAKAIANIAAAGRPVLFVGTKKQVREILVEEAERANSFYVANRWLGGMLT
                     NFQTIRQSIKRLDDLDAKAQDGTFQKLTKNEVLGLEKERQKLNAVLCGIRHMGRLPGA
                     LVVVDTKKERIAVAEANKLGIPVFAVVDTNCDPDLITYPIPGNDDAIRSVGLVLHVLA
                     DAVIEGMQNIQQPEQPEPAAASRESGER"
     gene            complement(936..1328)
                     /gene="rpsI"
                     /locus_tag="P5179_03725"
     CDS             complement(936..1328)
                     /gene="rpsI"
                     /locus_tag="P5179_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRS94360.1"
                     /translation="MESSVKSMTGRRKEATAMVRMMPGSGQITVNGRPVAEYFARPTL
                     VMMIEQPLKLTDTLGSLDISANCKGGGLSGQAGALRLGIARALVNENPDLRPTLKAAG
                     LLTRDPRMVERKKYGQPGARKRFQFSKR"
     gene            complement(1376..1816)
                     /gene="rplM"
                     /locus_tag="P5179_03730"
     CDS             complement(1376..1816)
                     /gene="rplM"
                     /locus_tag="P5179_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRS94361.1"
                     /translation="MATYGKGTYTESSKNVEPVWYVVDAENEVLGRLASRIAKVLRGK
                     HRVTYSPHMDLGDSVIVVNAEKVKVTGNKRNSMAYFHYTGYPGGGRIVPFEKMMQKDP
                     TEAVRHAVRGMLPKGALGFSLLGKLRVYAGSEHPHVAQNPQPLP"
     gene            complement(1986..3146)
                     /gene="carA"
                     /locus_tag="P5179_03735"
     CDS             complement(1986..3146)
                     /gene="carA"
                     /locus_tag="P5179_03735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRS94362.1"
                     /translation="MTTGSNEAYLALEDGTILEGLSFAAPGLRVGEVVFNTSMAGYQE
                     VLTDPSYARQIVTMTYPLIGNYGVSTADLESSHPWVDGFIVKEYCRWPSNWRSELPLG
                     DWLAHHGVIAMEGVDTRSLTKRLRVKGSLRGCLAAGVPADPHELVEKAREWPGMAGLD
                     CVPLVSRKDSCEWTTGLWDVRNNAEIAPPPPKYHVVAYDFGCKQNILRLLVHHGCRVT
                     LVPGNTPPEAVLDLKPDGVFLSNGPGDPEAVTYAISAVKHFVRESEATGLPVFGICLG
                     HQILGWALGGRTFKLKFGHRGGNQPVKDLTTGRVEITSQNHGFAVDPDSLGPEVEVTH
                     INLNDNTVEGLRHRRLPVFGVQYHPEASPGPHDARYLFARFTGLMEARRPDR"
     gene            complement(3359..4648)
                     /locus_tag="P5179_03740"
     CDS             complement(3359..4648)
                     /locus_tag="P5179_03740"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRS94363.1"
                     /translation="MESLILRGGHVLDPSQGIDEQVDVVIENGKVTDITPPGRKARGR
                     DVNVEGMFVAPGLVDIHVHLREPGYEYKETIETGANAAVAGGFTSIVAMPNTDPPPDT
                     AAAVLGFAARARNAACRVYTVGTITQGRQGQELSEMADLVAAGVVGFSDDGAPVSNSR
                     VLLNAMEYARPLGKAILSHCEVPELAEGGQMHEGRVSSELGIAGMPPIAESLGISRDI
                     ALAGYSGARLHICHVSAAASVDVIRSAKMAGSARITAETAPHYLALTDEAVRTFDSNA
                     KMNPPLRTRQDQEALKKALQDGTIDVIATDHAPHSHEEKQVEFDAAPFGIIGLETSLG
                     VCWTELVKTGVLTPMQLIEKMSTNPAQVCGLPAGTLKPGTAADVVVIDPDMRWVVAEP
                     FRSRSQNSPFIGCELQGRAILTIVGGTVRYDVGMLAK"
     gene            complement(4712..5665)
                     /locus_tag="P5179_03745"
     CDS             complement(4712..5665)
                     /locus_tag="P5179_03745"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRS94364.1"
                     /translation="MTKWTRKHLLGLADLTREEIGQILDSADQFVSVLDRPMRSVPTL
                     RGWTVVNLFFEDSTRTRISFELAEKRLGADIVNFSVSTSSVKKGETLRDTARNIEAMR
                     VDAVVIRHRSVGAPHFLSRHIQGSVINAGDGSHEHPTQGLLDLLTIRRHFGHIDGLRV
                     AIVGDIRHSRVARSDVWGFTTMGAEVVLCGPPTLLPKHTDNFPAKVTTRLEEALEGAD
                     VVYALRIQRERQAAGLFPSIREYSAQYGISSERMERAKPTAVLLHPGPINRGWELTDE
                     VTDGPRSLILEQVTHGVAIRMAVLYLTAGRDPAQLDLAPKP"
     gene            complement(5662..6240)
                     /gene="pyrR"
                     /locus_tag="P5179_03750"
     CDS             complement(5662..6240)
                     /gene="pyrR"
                     /locus_tag="P5179_03750"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRS94365.1"
                     /translation="MPETHEQTKSSGSPGPVVMTAAQMSRALNRMVHEILERNGGAEN
                     LALVGVRTRGYPLALRLASLIERVEGKRPPTGAVDVTFFRDDFRLRAKAPQEFTDIPF
                     AVDNKNIVLVDDVFFTGRTTRAAMETVMEFGRPRTIQLAVLVDRNHRELPIHPDYVAK
                     RVETALDEVIRVRLKEIDGEDQVLLVRGGVTP"
     gene            6635..7705
                     /locus_tag="P5179_03755"
     CDS             6635..7705
                     /locus_tag="P5179_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRS94366.1"
                     /translation="MREESLSFLKALLEAPSPSGYEQPAQRVWRDYVSSFADTVTSDR
                     HGNAIAAINPGGKPRVMLAGHCDELGFMINYINDQGFLNFLAIGGHDVAIVPGRRVTI
                     HTANGPVKGVIGKRAIHLMTQQERDDPKPKIEGYWIDIAVKSKDEAKELVAIGDPVTY
                     NVAFEELRNGIAVSRAFDDKAGAFVVAEALRLIAENRAGLKAAVYAVSTVQEEVGLRG
                     AQTSAFGIDPDVGIAVDVTHATDSPDMDKRKAGEVNLGGGPAISRGANISPIVFDRLV
                     DAAKKNEIAYQVEGAPRGTGTDANVIQLTRSGVATGLVSLPLRYMHTTVETIALSDME
                     QSAQMLATFCADLTPETSLIPQ"
     gene            complement(7803..9185)
                     /locus_tag="P5179_03760"
     CDS             complement(7803..9185)
                     /locus_tag="P5179_03760"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRS94367.1"
                     /translation="MKERVLDGQMSRRTFLGVGATGLAATALHGLLPSRSRADSTPRT
                     RTTAPDGRPCAVVRTAEFFGDEEMVLTFPHGWQVQTCYMDGHFAPPLTDDEIRAALRS
                     TVQTGRIAELAQGKRSVVITFDDCARPTPTGRVVPFVIEELHAAGIGDDQIYFHGSFG
                     THAAMYQQDFTAKLGADIVAKYPVWNHDCFNGMTQVGTTSRGMPVKLSKRFVDADFKI
                     CISGVKPHGMAGYGGGAKAIVPGVASIETARYAHEELNSIERTAVAYVNNPVRLGMEE
                     AARLANLDISVNIIMNGDRQVAGLFAGDVVGAHRAAVRVAHERYNTTLAQNADVVVSN
                     AYPQSVEASKELKYCRMSLRDGGTAVLIQDTPAGQRKIHYLGWSGDLERRALRRRSRG
                     LPVPQAAQVIVFNRWPCALDEMDYSPEVYHAKTWDEVVARLTRIHGEGTKVAVYPAAA
                     LAHEPMPLRM"
     gene            9787..12246
                     /locus_tag="P5179_03765"
     CDS             9787..12246
                     /locus_tag="P5179_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94368.1"
                     /translation="MLRKALPPKYAAWLSEQNIPESDILLCTDNDITFEGEYAERWVV
                     VTRDAVIMLRERDGQVQAARSFKMADIESARIDPRVGSGFLQVKVHGHWAEVARFSNA
                     RAIWFERIAAKITRFKETGEIILTEEDEKEQNRCPTCGLLLRSSTDICPRCINKNRVF
                     GRLLRMMQPYWPWALGTLLLVFTGIGLDLVPPQLSRILMDDIFVHDPARPYLMPEWAL
                     RLMNITPGTQMTPSEKVTLLFFVVFLGMAGVQLLRIGLNIVIGRLSVTVGTKITYDLR
                     HKLFSRLSELSVRYYDRHQVGILMQRVTGDTESLHGFVSQSTQGFLVNILLPVFIGAA
                     LFKMNPTLTLYVFIPAPFVIISTYYFWEFVYPNYYKYWDSHSKMNAMLNSVLSGVRVV
                     KAFAQEEREISRFNHLSGNVQASRQRVDKATGTFYPIVGFIFGLGGLIVWYVGGRYVL
                     EGKGMTLGTLTAFTSYLGMFYGPLSSLTYISSWLTGFMTSAQRVFEVLDTEPELQDTD
                     SALHLHICRGEIEFKNVTFGYDRYNPVIRDVTAHIKPGEMIGIVGRSGSGKTTFVNLL
                     CRFYEVDQGEISIDGISLRELHKDDIRRNIGLVLQEPFLFRGTIRENIAYGRPDADIE
                     NVMKAAKAANAHDFIMRLADGYDTKLGEYGSGLSGGERQRISIARALLYDPAILILDE
                     ATSSVDTESEKQIQEALAVATKGRTTIAIAHRLSTLRNSNRIIVMDDGRIIEMGTHDE
                     LIKKEGTYYRLVKIQTQLTSEPTVDRLELEKKEQEAAVSDGSSEKETEKENEKDSEEK
                     TDAESTEPSEDESSKDSEQTS"
     gene            12271..12816
                     /locus_tag="P5179_03770"
     CDS             12271..12816
                     /locus_tag="P5179_03770"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HRS94369.1"
                     /translation="MEELATQKPTESGYKGLVFLDPKDITIHESELAGMNVTVGGETF
                     EEVRASLALPITEPDRYVSLRIGASKGEEQEIGMIRDVNELSPENQVLVRRELRKRYF
                     LHVIYKILSLKEKFGFLYFEAETSKGLRKFAVRWEYNRVQEYSEFGRIIIDTDDNRYI
                     IPDLRKLSPEEYKNFTRYIYW"
BASE COUNT         2593 a         3804 c         3843 g         2798 t
ORIGIN      
        1 ccgctcgccc gattcacggc tcgccgccgc aggctcaggc tgttccggct gctggatatt
       61 ctgcatccct tcaatgactg cgtccgccag aacatggagc accaggccca cagaacggat
      121 cgcatcatca ttcccgggaa tcggatacgt gatcagatca gggtcgcaat tcgtgtcaac
      181 caccgcgaat acggggatgc ccagtttgtt cgcttcggcg acggcgatgc gttccttttt
      241 cgtgtcaaca accaccagcg cccctggcag acgacccata tgacgaatgc cgcaaagcac
      301 cgcgttcagc ttctggcgct ccttctcaag ccccagcacc tcgttcttcg tcagtttctg
      361 aaacgtcccg tcctgcgctt ttgcgtcaag gtcatccaac cgcttgatgc tctgccggat
      421 cgtctggaaa ttcgtcaaca tgccgccaag ccagcgattt gccacgtaga agctgttggc
      481 gcgctccgct tcttcaacga ggatttcacg aacctgcttc ttcgtgccga caaacagcac
      541 cgggcgtccc gccgccgcaa tgtttgcgat cgccttcgcg gcattctcca gaagcgcgat
      601 tgtcttgttc aggtccagaa tatgaatgcc gttccgttcc atgaagatga accgcttcat
      661 cttcgggttc cagcggcgcg tctggtgccc gaaatgaacc cctgcttcca gcaaatcctt
      721 catttccacg cgtgccaaag ccatcctcct tgcccaccct tcaaccgggt gaacctgttg
      781 tgcgtcgttt gccgccacgt cacgctatcc accccgatcc gcattcagac ggccagagcc
      841 gggaaacgct cactccccgc aagggagtga cgacgatcgg gagccgtgat cgtgtgaggg
      901 atacctgtaa ggcaatctgc caaacaggcg attgattacc gtttggagaa ctggaaccgt
      961 ttgcgtgcgc ccggctggcc gtactttttg cgttccacca tacgtgggtc acgggtcaga
     1021 agtccggcag ccttcagtgt cgggcgcaga tccgggtttt cgttgaccag cgcgcgggca
     1081 ataccgagcc tgagcgctcc tgcctgtccg ctcaacccac cgcctttgca gttggcgctg
     1141 atatccaacg acccgagagt atccgtgagt ttgagtggtt gctcgatcat catgacgagg
     1201 gtcgggcgag cgaagtattc cgcgacgggt cggccgttga ccgtgatctg gcccgagccc
     1261 ggcatcatgc ggaccatcgc cgtcgcttct ttgcggcggc cggtcatgct tttcacggag
     1321 ctttccatgt cttcgtgtct ttcctatccg gcagacggtt gacgtaacaa gtttgttacg
     1381 ggagtggttg tggattttgc gcgacgtgcg ggtgttcgga gcccgcgtag acgcgaagtt
     1441 ttccgagcag cgagaatccg agtgcgcctt tgggaagcat cccacgaacc gcgtggcgca
     1501 ctgcttctgt cgggtctttc tgcatcattt tctcaaacgg gacaattctt ccgccaccgg
     1561 ggtagccggt atagtggaag tatgccatgc tgttccgttt gttccccgtg actttgacct
     1621 tctcagcatt aacgacgatg acgctgtcgc ccagatccat gtggggcgag taggttaccc
     1681 tgtgcttgcc gcgaagcact ttcgcgattc tgctcgcaag gcgaccgagc acctcgtttt
     1741 ctgcgtcaac gacgtaccac accggctcta cgtttttcga actctcggtg taggtacctt
     1801 tgccgtacgt tgccatgcca tactccacct gcgttatatg ggaaacaccc gccgcagcgg
     1861 tggttgtgac aggagagtct cctgcgaccc ccatccggtc tgtggcgttc gcgttggaca
     1921 ctatgcgggc tgtacagatt tataaagtta cagtaatttg cgggtgtttg tcaatagcgg
     1981 gtcagtcacc ggtcgggccg tctggcttcc atgagaccgg tgaatcgtgc aaaaaggtac
     2041 cgcgcatcgt gcggcccggg ggatgcctcc ggatggtact gaaccccgaa gacgggcaac
     2101 cggcggtggc ggagcccttc gacggtgtta tcgttgagat tgatatgggt gacctcaact
     2161 tcggggccaa gggaatccgg gtcgacggca aatccgtgat tttgagaggt gatttctacc
     2221 cggccggtcg taaggtcctt tacaggctga tttccgcccc tgtgaccaaa tttgagtttg
     2281 aatgtcctcc ccccgagtgc ccagcccaga atctgatggc cgaggcagat gccgaacacc
     2341 ggtaagcctg ttgcctcgct ctcgcgcaca aagtgcttca ctgcagatat tgcgtacgtc
     2401 actgcttcgg ggtcgcccgg accgttggaa agaaacaccc catccggttt gagatccagc
     2461 accgcctcag gaggagtgtt tccgggaacc agggtgacgc gacatccgtg gtgaaccagc
     2521 aagcgaagga tgttctgttt gcacccgaaa tcgtacgcga ccacatggta tttcggcgga
     2581 ggtggagcta tttccgcgtt gtttcgcacg tcccacaacc ctgtcgtcca ttcgcatgag
     2641 tctttgcgcg aaacgagggg aacgcaatcc agccccgcca tgcctggcca ttcccgtgcc
     2701 ttttccacca attcgtgagg atctgccggc acaccagccg cgaggcaccc gcgcaacgaa
     2761 cctttcaccc ggagcctttt ggtcagcgaa cgcgtgtcaa ctccttccat ggctatgacg
     2821 ccgtggtggg ccagccagtc tccgagtggc aactcactcc tccagttgga gggccaccgg
     2881 cagtattcct tgacgatgaa gccatccacc cagggatggg aggattcgag gtccgccgtg
     2941 ctgactccgt agtttccgat aagcgggtag gtcatcgtca cgatctggcg ggcgtaggag
     3001 gggtctgtca gcacttcctg gtagcctgcc atggaggtgt tgaatacgac ttcgcctacc
     3061 ctcagccccg gcgcagcgaa ggagagccct tcgaggatgg tgccgtcttc cagcgcgagg
     3121 tacgcttcgt ttgatccggt tgtcatgatt tccctgctga agttcgcccg tcagatttgg
     3181 tcccgctgtg attcccggaa cgctgtacga gccggcgcgc gactgaatgc ccatgatcac
     3241 gtacgaggca tgccgtttga tactgcgtgg cacgcacccg gagcacattc ccgtgccacc
     3301 cgcgagcccg gcgaaccgtt ctcgagaccg actggcggcc ctgcgtgcgc gttgtgtgtt
     3361 attttgcgag catgcccacg tcgtatcgaa ccgtgccacc tacgatggta agtattgccc
     3421 ttccctgcaa ctcgcatccg atgaaggggc tgttctgaga tcgagaacgg aagggttcgg
     3481 caactaccca gcgcatgtcg ggatcgatca cgaccacgtc ggcagcggtg ccgggcttca
     3541 gtgttcccgc cggaagcccg catacctgcg cggggttggt ggacattttt tcgatgagtt
     3601 gcatcggggt aagaacaccc gttttgacga gttccgtcca acacacaccc agggaggttt
     3661 ccagtccgat gatgccgaac ggcgcggcgt caaattccac ctgcttctct tcgtgagaat
     3721 gtggtgcgtg atccgtggcg atgacgtcga tggttccatc ctgcagtgcc tttttcagcg
     3781 cctcctggtc ctggcgagtg cgaagaggcg gattcatttt cgcattgctg tcgaatgttc
     3841 tgactgcctc gtcggtaagc gccagataat gaggtgctgt ttccgccgtg atccgtgcgc
     3901 tgcccgccat tttcgcgctt cgaatgacgt cgacacttgc ggccgcagag acgtggcaaa
     3961 tgtgaagccg tgcgccgctg taccccgcaa gcgcgatatc gcgagaaatg cccaacgatt
     4021 ccgcgatggg tggcatcccg gcgatcccga gctccgaact caccctgccc tcgtgcatct
     4081 gcccgccttc ggcaagttcg ggcacttcac aatgcgagag gatggctttc cccagcggcc
     4141 gcgcgtattc catcgcattg agcagcacgc gggaattgct taccggcgcc ccatcatcgg
     4201 aaaaaccaac cacgccagcg gcaaccagat ccgccatttc gctgagttcc tgcccctgcc
     4261 tgccctgggt gattgttccg accgtatata cccgacaggc ggcattccgc gcccgtgcgg
     4321 cgaatcccag caccgcagcg gcggtatcgg gcggcgggtc ggtgttgggc atcgccacga
     4381 tggaggtgaa tccgccggcg accgcagcat tcgcgcccgt ctcgatggtt tccttgtatt
     4441 cataaccggg ctcgcgcagg tgcacgtgaa tatccacgag gcccggggcg acaaacatgc
     4501 cttcgacgtt cacgtcccgc ccacgggcct tacgccccgg cggcgtaatg tcggttactt
     4561 tcccgttctc gattaccacg tccacctgct cgtcgatccc ctgcgaagga tccaggacat
     4621 gcccgccacg cagaatcagt gattccatca gcaacctcgc tcattcgcgg taatgttggt
     4681 ttccgccacc gaaaagacat caaccaggag ttcagggttt cggcgcaagg tcgagctgcg
     4741 ctggatctcg ccccgcagtg agatagagca ccgccatgcg gatggccacc ccgtgcgtca
     4801 cctgctccag gatcagggag cgcggcccgt ccgtcacctc atcggtgagt tcccaccccc
     4861 ggttgatcgg cccggggtgg aggagtacgg cggtcggttt cgcccgttcc atgcgttcgc
     4921 tcgagattcc gtactgcgcg ctgtattccc tgatggaggg gaacaacccc gcggcctgcc
     4981 gttcccgctg aatccgcagt gcatacacta catcggcgcc ctcgagcgcc tcttcgagtc
     5041 gagtagtcac ctttgccggg aagttatccg tgtgttttgg caagagcgta ggagggccgc
     5101 acaggacgac ttccgcgccc atcgtggtga atccccagac atcagatcgc gccacccgac
     5161 tgtgccggat atcgccgacg attgccacgc gaagcccgtc tatgtggccg aagtggcgcc
     5221 ggatcgtcaa caggtccaac aagccctgag tcgggtgctc gtgtgagcca tcgccggcgt
     5281 tgataacgct tccctggatg tgccgtgaga ggaaatgcgg cgctccgacc gagcggtgcc
     5341 gtatcaccac tgcgtccacc ctcatggctt caatgttccg ggccgtgtcc ctgagcgttt
     5401 cacccttttt cacactcgaa gtggacacgc tgaaattgac gatgtcggca ccgagtcgct
     5461 tttccgcgag ttcgaacgat atccgggttc gcgtgctgtc ttcgaaaaac aggttgacca
     5521 cggtccatcc gcggagcgtg ggaacggagc gcatggggcg gtcgagaacg gatacgaact
     5581 ggtcggctga atcgagaatc tggccgattt cttcgcgggt gagatccgcg agccccaaca
     5641 ggtgcttcct ggtccatttg gtcatggtgt gactccgcct ctgacgagaa gtacctgatc
     5701 ttccccgtca atttccttca atcggacacg gatgacttcg tccagagcgg tttccacccg
     5761 ttttgcgaca taatctggat ggatgggcag ttcgcggtgg ttcctgtcta ccaacacagc
     5821 aagttggatg gttctgggcc gtccaaattc cataacggtt tccattgctg cccgtgtcgt
     5881 cctgccggtg aagaacacat cgtcaacaag gacgatgttt ttgttgtcga ccgcgaacgg
     5941 aatgtcggtg aattcctggg gtgctttcgc gcgcaatcgg aagtcgtcgc gaaaaaacgt
     6001 gacgtctacc gcgccggtag gcggcctctt gccttcgact ctctcgatca gcgacgcgag
     6061 acgcagcgcc agtggatacc cgcgagtgcg cactcccacc agggcaaggt tctccgcacc
     6121 cccgttgcgt tcaagaatct cgtgaaccat acgattgagc gccctggaca tctgggccgc
     6181 cgtcatcacc accggaccag gggaacccga gcttttggtc tgttcgtgtg tttccggcat
     6241 gtttcactcc acgtctcgaa tttcagcctt cgcggacggg atcagccccg attcagccac
     6301 cccgtccgaa cgaccgggca aaaaaaaacc cgcggggcat cctgcccggc gggtatcgcc
     6361 acgtgcctgt ctgcacaatc gtctgacttt catcgcctgc catgcaacac agtgcgtttt
     6421 cggctgttgc gcgctgttat gtagaagata cggtgttgag cagttcccgt caacgaaagg
     6481 ttggcacagg tgtgagcacc cgcggggcag gaaaggggtg cggctggacg cgttgcgcgg
     6541 aatcacgttg ctcgcctatc ttttttctgc atctatctgc ttttgcacta cgttcccgcc
     6601 catcgcggaa tcccgcggac tcaaggagct atccatgcgt gaagaaagcc tttcgttcct
     6661 caaagcgttg ctcgaggcgc cgagcccatc gggctacgag cagcctgcgc agcgcgtctg
     6721 gcgagattat gtgtcttcat tcgccgacac ggtaacaagc gaccggcatg gcaacgcgat
     6781 tgcggccatc aaccccggtg gaaaaccgcg cgttatgctt gcgggacatt gtgacgagct
     6841 tggtttcatg atcaactaca tcaacgacca ggggtttctg aatttccttg ctatcggcgg
     6901 acacgatgtt gcgatcgttc cgggccgacg tgtaacgatc catacggcga acgggccggt
     6961 aaagggcgtg atcggaaaac gggcaattca cctgatgacg cagcaggagc gagatgaccc
     7021 aaagccgaag attgaagggt actggattga tatcgcggtg aagagcaaag acgaagcgaa
     7081 ggagcttgtt gcgataggtg atccggtgac ctacaacgtc gcgttcgagg agttgcggaa
     7141 cggcattgca gtatcccgtg cttttgatga taaggccggc gcgttcgtgg tggccgaggc
     7201 gctgcgtttg atcgcggaaa accgggctgg tctcaaggcc gcagtatacg ccgtcagcac
     7261 ggttcaggag gaagtcggtt tgaggggagc gcagacgagc gcgttcggaa tcgaccccga
     7321 cgtagggatt gcggtggatg tgacccacgc aaccgattcg cccgacatgg acaaacggaa
     7381 agcgggtgag gtgaatctcg gcggcgggcc agcgatttcc cgcggggcaa acataagccc
     7441 gatcgtgttt gaccggctcg ttgacgccgc gaaaaagaac gaaatcgcgt accaggtgga
     7501 aggtgcgccc aggggcacgg gaaccgacgc caacgtaatt cagttgacac ggagcggcgt
     7561 ggcgacgggg cttgtgagcc ttcctttgcg gtacatgcac acgacggtgg aaaccatcgc
     7621 gctcagcgat atggagcaat cagcgcagat gctggcaacg ttctgtgctg atctgacacc
     7681 tgagacgagc ctgatacctc agtaacgggc tcgcccaaac gcacaaaaaa aaacgagggg
     7741 catgagtttt cgcgtcatgc ccctgttttt ttgccggaag caattgcctg aagaagttgc
     7801 gtctacatcc gcaagggcat cggttcgtgc gcgagtgcgg cggcggggta cacggcgacc
     7861 ttcgttcctt cgccgtgaat ccgcgttagc cgggcgacaa cttcatccca cgttttggcg
     7921 tggtagacct cgggggagta atccatctca tccagtgcgc acggccaccg gttgaagacg
     7981 atcacctgcg cggcctgggg aaccggcagc ccgcgtgaac gccgccgcaa ggcgcggcgt
     8041 tcgagatcgc cgctccatcc cagataatga attttgcgct ggccggcggg ggtatcctga
     8101 atcaggaccg cggtaccgcc gtcgcgaagg ctcatgcggc agtatttgag ctctttgctg
     8161 gcctcgacgc tctgcgggta ggcattcgag accacgacat cggcgttctg ggcgagtgtt
     8221 gtgttgtaac gttcatgggc gacccggacc gcagcgcgat gtgctcccac cacatctccc
     8281 gcaaaaagcc cggcaacctg ccggtctccg ttcatgatga tgttcacgct gatgtcgaga
     8341 ttcgcgagcc tcgccgcttc ttccatgcca aggcgaacag ggttgttcac gtacgcaacc
     8401 gcggttctct cgatgctgtt gagctcctcg tgcgcgtacc gcgcggtttc gatggaagcg
     8461 acacccggaa cgatggcctt cgcgccgccg ccgtaccccg ccattccgtg cggcttcacc
     8521 ccggagatgc agatcttgaa atccgcatcg acgaaccgtt tgctcaattt gaccggcatt
     8581 ccgcggctgg tggttcctac ctgcgtcatg ccgttgaagc aatcgtggtt ccagacgggg
     8641 tattttgcca cgatatccgc gccgagtttg gcagtgaaat cctgctgata cattgccgca
     8701 tgggtgccga aggagccgtg gaaatagatc tggtcgtcgc caattccggc ggcatggagt
     8761 tcttcgatga cgaacggaac cacgcgaccg gtgggcgtcg gccgggcgca gtcatcgaag
     8821 gtgatcacga cagaacgctt cccctgcgcc agttctgcga ttcgaccggt ttgaaccgtg
     8881 gatctcagcg ccgctctgat ttcgtcgtcg gtcagcggcg gcgcgaaatg cccgtccata
     8941 taacaggttt gcacctgcca cccgtgcggg aaggtgagga ccatttcctc gtcgccgaaa
     9001 aactccgcag tgcgcacgac cgcgcagggg cggccgtccg gtgcggtggt cctcgtccgg
     9061 ggtgtgctgt ccgcccggct tctggaaggc aggagtccgt ggagcgccgt agctgccaac
     9121 ccggtggctc caaccccgag gaatgtgcgc ctgctcatct ggccgtcaag gactcgttcc
     9181 ttcatagttt tccccttcct gatccggcgg atggtacttt gcgccttcgc atgcgcgacg
     9241 tggttgagga agaatatgga taatgcaagg aagcgcggca acatccccgt gcggcggcct
     9301 tgtcaaaatg ccatgtatcg cgtagctttg aggatactag aacgcgctgc aatgaaggca
     9361 gccgaggttc aggcgcgctt actttcctga agaagcgctc acgcgtccca tgaagtgaaa
     9421 agaatcgctg ggagacttgt tcggaggcgc cgcgtacgtt ggttttctgc gacgtgccgg
     9481 cgttttccca cgaggcgttt gcgagacgga caaccgggga acgatgagcg ctgaagtgac
     9541 cgcgtttcca gcgtttgttg cgggggaagg gccctccctg gggggctggc ctccggcagg
     9601 gcgtagcacg ttaagaaggt atgttgaatc ccacctgaag gttcccgaag gcagccgcat
     9661 tgtgaatgct catggtcggc acgcgcgtcg ggttctccca tctgtattcc cactatttcc
     9721 atggacttcc tgtaaggcat acgctgcgaa cgtttccgcg cagcgctggc gaaaggcata
     9781 atccgcatgt tgcgaaaagc attgccaccg aagtatgcgg cctggctttc tgagcagaat
     9841 attccggaga gcgacatcct gctctgcacg gacaatgata tcacattcga aggcgagtac
     9901 gcggaacgct gggttgtcgt aacgcgcgac gccgtgatca tgctccgaga gagggacggg
     9961 caggttcagg cggcgcgcag cttcaaaatg gctgacatcg agtccgcccg gatcgatccg
    10021 cgtgtcggca gcgggtttct gcaggtcaag gttcacggcc actgggcgga ggtggcgcgg
    10081 ttctccaacg cgcgggccat ctggttcgag cgcattgcgg cgaagatcac ccgtttcaag
    10141 gagacgggcg agatcatcct gaccgaagag gacgagaaag aacagaaccg ctgcccgacg
    10201 tgcgggctcc tgcttcggag ctctacggac atctgccctc ggtgcatcaa caagaatcgg
    10261 gtgttcgggc gattgctgcg aatgatgcaa ccgtactggc cgtgggcgct gggaacgttg
    10321 cttcttgtgt tcacgggtat cgggctggac cttgttccgc cgcagttgtc gcgcatcctg
    10381 atggacgaca ttttcgtgca cgacccggcg cgcccgtatt tgatgccgga gtgggcgctg
    10441 cggctcatga atattacgcc gggaacacag atgaccccgt cggagaaggt gactctgctt
    10501 ttcttcgtcg tgttcctggg catggcgggg gttcagcttc tgagaatcgg actgaatatt
    10561 gtgatcggtc ggctttccgt caccgtcgga acaaaaatta cctacgattt gcggcataag
    10621 ctgttttcga ggctttccga gctttcagtc cggtattacg accggcacca ggtcggcatc
    10681 ctcatgcagc gggtgacggg tgacacggaa agcctgcatg gattcgtgtc ccagtcgacg
    10741 cagggcttcc tggtaaacat actgcttccg gtgttcatcg gcgctgccct gttcaaaatg
    10801 aatcccacgc tgacgttgta cgtgtttatc cctgcgccgt ttgtgatcat ttcgacctac
    10861 tatttctggg agttcgtgta cccgaactac tacaagtact gggacagcca ttccaaaatg
    10921 aacgcgatgc tgaactcggt gctttccggt gtccgggtgg tgaaggcatt tgcacaggaa
    10981 gagcgcgaga taagccgttt caaccatttg agcggcaatg tgcaggcatc gcgccagcgg
    11041 gtggacaagg cgacgggaac cttctacccc atcgtcggct tcattttcgg tctgggcggt
    11101 ttgatcgtct ggtacgtcgg tgggaggtat gtgcttgaag ggaaggggat gacccttggt
    11161 acgctgacag cgttcacctc ctaccttggc atgttctacg gcccgttatc cagtctgacg
    11221 tacatctcct catggctcac cggcttcatg acgtccgcgc agagggtttt cgaggttctc
    11281 gacacggaac cggagcttca ggacactgat tcggcgctgc atctgcacat ctgccgcgga
    11341 gaaatcgaat tcaagaacgt cacattcggg tacgataggt acaatcccgt gatccgcgac
    11401 gtcacggcgc acatcaaacc gggtgagatg atcggcatcg taggccgctc cgggtcaggg
    11461 aagacgacgt ttgtgaacct tctctgccgg ttctatgagg ttgatcaggg cgaaatatcg
    11521 attgacggga tcagcctccg ggagcttcac aaggacgaca tccgtcgcaa catcgggctt
    11581 gttctccagg aaccgtttct tttccgcgga accatccgcg aaaatatcgc ctacgggcga
    11641 cctgacgcgg acatcgagaa cgtgatgaaa gcggccaagg cggcgaacgc ccatgatttt
    11701 atcatgcgcc ttgccgacgg ttatgacacg aaactcggag agtacgggtc gggcctttct
    11761 ggaggcgaac ggcaacgtat cagtattgcg cgcgcactgc tttacgaccc ggcaattctg
    11821 attctggacg aggcaacctc ttcggttgac actgagtcgg agaaacagat tcaggaagcc
    11881 ctcgccgttg ccacaaaggg gcggacgacg attgcaattg cgcaccgcct ttcaacgctg
    11941 cgcaattcca accgcatcat cgtgatggac gatggtcgca tcattgagat gggaacgcac
    12001 gacgaactca tcaaaaagga aggcacgtat taccgtctgg tcaaaatcca gacccaactg
    12061 acctctgagc cgaccgtgga ccgccttgag cttgagaaga aggaacagga ggcagcagtt
    12121 tccgacggaa gttccgaaaa ggaaacggaa aaagagaatg aaaaggactc cgaggaaaag
    12181 actgacgccg aatccactga gccatctgaa gacgaatcgt ccaaagattc cgagcaaacg
    12241 tcgtgaacgt ttcgcacgtg gagtactacc atggaagaat tggctacaca gaaacccacc
    12301 gaatcaggat ataaagggct ggtgttcctg gatcccaagg acatcaccat ccatgagtct
    12361 gagcttgccg gaatgaacgt gacggtaggc ggggaaacat tcgaggaagt ccgcgcctcc
    12421 ctcgcattac cgattaccga acccgaccgg tacgtaagcc tgcggatcgg cgccagcaag
    12481 ggggaggagc aggaaatcgg aatgatccgc gatgtgaacg aattgtctcc cgagaatcag
    12541 gtgctggtac gacgtgaact gcggaaacgg tattttcttc atgtcatcta caaaatcctc
    12601 tcgctgaaag agaagttcgg cttcctttac tttgaagcag agacaagcaa ggggctgagg
    12661 aaattcgccg ttcgctggga atacaacagg gttcaggagt attccgaatt cggccgcatc
    12721 atcatcgaca cggacgacaa ccgatacatc atccccgatc tgcgcaaatt gagccctgag
    12781 gaatacaaga acttcacgcg gtacatctac tggtaagacg acggaacgga aaagagaaaa
    12841 aaggaaacag acgccgcacc acggcgtctg tttccttttg tgaccggttg cgcagccttc
    12901 acccgcctca cttgcccgca gcaatccttg cggtgagcgt cttcttggtg tcctcgtaga
    12961 gccgcaccat tcgttccagc gacgcatcgt cgtacggcgt ggagagcgtg cgcaggtact
    13021 caattcctcc gttgacaa
//
LOCUS       DAOTNG010000049        12965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4638_length_12965_cov_5.056313, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000049 DAOTNG010000000
VERSION     DAOTNG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12965
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_12965_cov_5.056313"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2348
                     /locus_tag="P5179_03775"
     CDS             <1..2348
                     /locus_tag="P5179_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94370.1"
                     /translation="GGIVVGYVYKKEMTITPALRPYFRDQTFDMKLTLTPTEKLKIRL
                     SYMRGSSASSALSSLGNNAELSETRAVGELVEPTALRDASMLYVTLSGGKGSSNNKLS
                     LSNSCFLDGTFGQWGVAVTYTFNPATYMTLSLARSSSQTDMTRDLPRVNVNASYDPAN
                     LASGYKPPSSFGYRGWLGMFYLWSDTGGTAAADLPSSLQDALTPGRLILNDPFSTPNY
                     STAVLGQGRYVTRTFFADTPTPAVVVSPQGYIQDGYADLSGTYYLGTGAEAVLNATAD
                     QTIVKGDITHAWRAHTFKTGFEFITSELGFHAERALGLSVPGRNQNHVDYGGKYPTPH
                     PSIFGAFAQDKYESQGLIANAGVRIERFDANYPVYVQNIFTDIALIPYGAKAYYDSIA
                     IARGWDVSRWGGVPTYYPLRDTLRKYGLGDPPEPWDIYEALPHGPNKVFWRIEPRFGV
                     SHPVSDRTKFFFNYGLFYSMQKPAVMFSFQTDERLGQVGKIQEIRYPNLRPARTTMYE
                     VGIEHVLPHQIIWTTRGYAKYNVDQVSMLYCTVFQGNRMFRNANYEEVRGLEIKVARS
                     SGRFLNGWFTYETYSTRAGEVGLYSINPNMAQSSYFTAYARSNEPLANIRGFVRLGTP
                     RDWGSWKGGWGIGVMETYREGSEVIYRPDPTIPLRELPAENYMKTVDYWYTNLKLDKT
                     IDLQGGRTLSAYMDVTNLLNTKYMSSGGMASYNDYLNYVFNRRQAGEKNLRVYDPSTE
                     EALTRPYKDAGGNWKAPISPRTEWLLYPTARVVRFGLRFDF"
     gene            complement(2413..6846)
                     /locus_tag="P5179_03780"
     CDS             complement(2413..6846)
                     /locus_tag="P5179_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS94371.1"
                     /translation="MDPTSLTERAMRQWRITVTVVAVCVVAGVYSFFTMQRQEFPAFT
                     IRQGLVIGVMPGATSEEVEDQLARPVEDFLFSFNEVNKAKTYSVSKDGQLVVFVEVKE
                     DLDGIQTAAFWAKLRHGLNELKAQKLPGGVLALVGNNEFGDTSALLFTLVAEGKSPRD
                     LREYMKVLESHLRRIEATAKLRVIGEQDEVISINISRDRVARYGIRPATLWASLQGLG
                     GLPAYARLDGDELERPIYVRKVLRSENELAETILLSLPTGQHVRLKDVAEIKREYGHD
                     DAYVRFNGKTALVLSIEMQPGNDITRFGDEVDRALEETLHELPPGVTISRVVDQPKVV
                     RTSVNHFLRDFGLAIASVILVTMLLLPVRVAAVAAITIPVSIAITLAILNALGVELQT
                     VSLAGLIVVLGMIVDNAIVVIDDHVDKLDRGMDAWSAAWMSAHDLAVPVFTATIAIIL
                     SYVPMPRFLTGVAAEFLGSLPVTIAVALSVSLLIALLLLPIMNSQFVRRGLRKRSGQR
                     SMLERIQCVFDRALDAVFRHPWLTVAGGIGSVLIAVVIALRIPQQMFPKVDRDQFAVE
                     VYLPTGRSLKQTDAVMRRLEAELLADKRVVNVTSFVGQSAPRFHTLYAPYIPRRNFGQ
                     LLVNTIDADATLDVLRESEERLRGAFPECWVRWKQLEFQVGFPVEVRLSGNDAEALKT
                     TGAKIESYARGIAEVTWVHNDWEEALQSVDVMPDADACARLGVPPAMLQASLALGTQA
                     YRVGTIWEGDYPVRVLVRDDSGERGSVEGLRQQYVSSILGATVPLEQIASLRPAWHEG
                     AMVRRNGVRTLTVFIDIKKDALASEMQEKIEKYVSGLDLPGIRVSYGGEKELSTQIFS
                     PMTISMLVSIGLIFLVVLCQFQRFLTTLVVMLSMPLTLMGAFLGLVIMGYPFGFTAFV
                     GIIGLMGIAVRNGIILVTYAEELRVKQGLSAMEAARAAGKRRMRPIYLTSTAAAVGVV
                     PMILSRSTLWGPLGTVTFFGLLTAMVLTLFVLPVTYALVVGREDRRHEPSPKAPVVTG
                     LLLGLALAVSPASADETPLTLQECKELALQNNAQIRESNLEIEAARETRRAAYTKYFP
                     QISVSATGAVAVDPLARIHTSGGNLPVYNGNPATIPTATQFAYFPSGEMAIAGRGFVA
                     SAAVIQPLFAGGRIVSGNRLADLGVHVAKDRAEISKRDVLAQTEEKYWRLVALREKLR
                     TLDAYEALLEALDKQVTDAVQAGLLTRNDQLKVRLKRSEASVDRQRLENGLALATRDL
                     RRHIGLPEGGALHLVDTLACPDDPSILAQERAGALDRRPEIRLLSRAIRAEQLQNSLT
                     RGGMLPTVSVGAIGLRQDIRGMISQTNGLLMGMVNVPISGLWEGRHTTASQHRRVEIA
                     ENRLAETRELLDLQIENAWTDLTAAWRSNEVANEAVDQADVNLAEVSDRYRNGLVTFS
                     DLLEAQVLRQKATDSRIDARADYWLKRAAYLRAVAAADGSE"
     gene            complement(6850..7926)
                     /locus_tag="P5179_03785"
     CDS             complement(6850..7926)
                     /locus_tag="P5179_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS94372.1"
                     /translation="MVWTTPPKRVLLGIVASLPTLCLSPFFAACGNKVQKTTSVEPGV
                     FVKPVTRVGNGRELHLSGTLAAERSIALSFATLGTVEQVMVEEGQEVKKGDVLAKLVS
                     RSYEDALAMAEANLNQAEDAYRRLEPMHRNGTLADVKMVEVETARQQARLAASIARKN
                     LDDTSLRSPVNGVIADRNVEPGASASPVLPAFTLVQAKTLLATAPVPETQVSKIRRGM
                     SARVVIPALQKTVEGSVREISVVANPLTRTYDLKVAVANPTGELRLGMIADVYIKVDG
                     EVENLVIPPEAIRVDEVGNPYVYVVAADRRLQRRPVRVGGFVGEGTMVTAGVRAGESL
                     VVSGTPMLADGMTVRVLAPHANGE"
     gene            complement(8018..8650)
                     /locus_tag="P5179_03790"
     CDS             complement(8018..8650)
                     /locus_tag="P5179_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS94373.1"
                     /translation="MNDTEMSGVGDGTRAPADTGEKDRKTEIREKARSVFLRYGYRKA
                     TLDDIGKACGLSKTAIYHYFPDKEAVFAEVVRAESGVLLSKMRAAAASETDPKARLAE
                     VIRTRFRAMSEILNEVVGDARHEVREFLPKTAHAREQFFHEEGKLIEEILEDGGRQGV
                     FRVLRTRNGPLLLIAALQGVENGLAGLSDSLSIEEAIDEMLEILFDGLCR"
     gene            complement(8988..9476)
                     /locus_tag="P5179_03795"
     CDS             complement(8988..9476)
                     /locus_tag="P5179_03795"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRS94374.1"
                     /translation="MGAGSFVRRRVISPIMDLLKQGVTPEKIALSMAVGGALGVFPVL
                     GSTTILCTLAAFLFRLNMPAIQIVNYVMFPLQLLLLIPFIRFGEWIFHAAPIPFSASE
                     MAAMFRSDFQGSMGLLWVTGLQAIVAWCAFMPPAAVLVYLVLAPGLRRIQARFRRTDG
                     PS"
     gene            9654..11108
                     /locus_tag="P5179_03800"
     CDS             9654..11108
                     /locus_tag="P5179_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS94375.1"
                     /translation="MRTAGNPNLLFIMTDQQRRDALSCMGSRFVHTPSLDRLAVEGVR
                     FTNAITPSPICVPARASVLTGLPIHHTGCITNPDRLRTPASDLTTFDDALTAHGYTAE
                     YIGKWHAPEALLRPYSRLPTSVSLDTFYRGYLAEKGYGSPHQGASHPYISPLSGQPYD
                     PDPMDHVYRSLNLGRGHSRPEPGLPFGRDSVRAEHSVSAMIADETIRSIEKLKYVPFS
                     ITSSFLFPHDPLIVPRPYCDSVRAADMATPETFDDARMNTPYKDFVWQMDDLERRHVH
                     LLMARYYAAVQEVDHHIGRILDALDTSGLTDNTLVVFTSDHGEMLGDHGLMTKFVHYR
                     EAVGVPLLMRMPGRIPEGSTVQAPVCLTDLFATISDYLGVTPPDRWGRSLQSWIAGDA
                     PEPDPIVFSQFEHRNVMAQTCAWKYVWSNHADEVDMLFDLTRDPGEVNNLHGANPDRA
                     RYLQQAAAMKERLAEWMEETEHPWLEQLAATPVR"
     gene            complement(11155..11739)
                     /locus_tag="P5179_03805"
     CDS             complement(11155..11739)
                     /locus_tag="P5179_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS94376.1"
                     /translation="MAGTVKVMLLGDSIRMSYQPLVARLLEGRAEVLGPADNCQYSLY
                     TLSSLARWANELGKPDIVHWNNGLHDAGHNPQRHPIQIPIDMYRANLAHILDALSRLT
                     DKIIWATTTPVHPERPFRADAWSWRNEEIDRYNRAALELMEERGIPVNDLHAVAWSNT
                     SEYLGPDQLHLSEAGKQACAEAVAESVSRLLPSS"
     gene            complement(11953..12321)
                     /locus_tag="P5179_03810"
     CDS             complement(11953..12321)
                     /locus_tag="P5179_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94377.1"
                     /translation="MPDHIRNLTIMQTDTAVVLSALGGPEVTVITDGRRRDVVWLDGN
                     NAKVKAEWTDNGLVIERREESGPTVRETYLRYPRSTRLSVEMEIKGSNLPRAMKLVRL
                     YDLLAAPEDTSHSILGDPMW"
     gene            complement(12297..12503)
                     /locus_tag="P5179_03815"
     CDS             complement(12297..12503)
                     /locus_tag="P5179_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94378.1"
                     /translation="MKLRRGSRIKVCEGATLVECREAREDRRAECRAVRIWEVVFRAA
                     GRVMEAPRVARVLSAPACPTTFET"
BASE COUNT         2709 a         4017 c         3646 g         2593 t
ORIGIN      
        1 tgggtggcat tgtggtgggg tatgtctaca agaaagagat gactatcacc ccggcattgc
       61 gtccctattt ccgcgatcag accttcgaca tgaagctcac cctcaccccc acggagaagc
      121 tcaagatacg actctcctac atgcgagggt cgagcgcttc gtcggcattg tcttccctcg
      181 gcaacaatgc ggagctctcc gaaacaagag ctgttggcga gcttgtggaa ccgacggcgc
      241 tgcgcgatgc cagcatgtta tatgtgaccc tttcgggagg caagggctcg agtaacaata
      301 aactcagtct ttcgaattcc tgtttcctcg acggaacgtt cggtcaatgg ggagttgctg
      361 tcacctacac gttcaatccg gccacgtata tgactctctc cctcgctcgt tccagttcgc
      421 agaccgacat gacccgggac cttccccgtg tcaacgtgaa tgcgtcgtac gaccccgcga
      481 acctggcctc cgggtacaaa ccgccaagtt cgttcgggta ccggggctgg cttggaatgt
      541 tctacctctg gtccgatacc ggcggcactg ccgccgcgga tctgcccagc agccttcaag
      601 acgcgctgac cccgggccgg ctcattttga acgacccgtt ttccacgccc aactattcca
      661 ccgcagtgct ggggcaaggg cgttacgtga cacgaacctt cttcgccgat acaccgactc
      721 ctgccgtggt agtgtctccg cagggataca ttcaggacgg atacgccgac ctgagcggca
      781 catactatct cgggacaggt gcggaagcag tgctcaacgc gactgcggac caaacgatcg
      841 tgaaaggcga tattacccac gcgtggcgcg ctcacacatt caagaccggt tttgagttca
      901 tcacaagtga acttggcttc cacgcggagc gggcgctcgg tctctccgtt cccggccgaa
      961 atcaaaacca tgtggactat ggcggaaagt accccacgcc tcaccccagt atcttcggcg
     1021 cttttgcgca ggacaagtac gagtcccagg ggctcatcgc caacgccggg gtacgaatcg
     1081 agcgcttcga tgccaactac cccgtatatg tccagaatat cttcacggat atcgccctca
     1141 ttccgtacgg agccaaggcg tactacgaca gcatcgccat cgcacgcggt tgggacgtga
     1201 gccggtgggg cggagttccg acgtactacc cgttgcgtga tactctgcgc aaatatggcc
     1261 ttggcgatcc acccgaaccg tgggatatct acgaggccct tccccacgga cccaacaagg
     1321 tgttctggcg catcgaaccg cggttcggtg tatcacaccc ggtgagcgac cgtacgaaat
     1381 tcttcttcaa ctacggcctc ttctattcga tgcagaagcc cgcagtgatg ttcagcttcc
     1441 agacggatga acgcctcggg caagttggca agatacagga gatccgatat ccgaaccttc
     1501 gccccgcacg aaccacgatg tacgaggttg ggatcgagca tgtcctgccc caccagatca
     1561 tctggacgac ccgcggatac gccaagtaca acgtggacca ggtctctatg ctttactgca
     1621 cggttttcca gggcaaccgc atgttccgca atgcgaacta cgaggaggta cgtggtctgg
     1681 agatcaaggt tgcacgaagc tcagggaggt ttctgaacgg ctggttcacg tacgaaacct
     1741 actcgacgcg ggccggcgaa gtaggtctgt acagcatcaa tccaaacatg gcacagtcga
     1801 gttatttcac cgcgtatgcg cgctccaacg aaccgctggc gaacatacgg ggtttcgttc
     1861 gtctgggtac cccacgcgat tggggctcat ggaaaggcgg atggggtatc ggcgtgatgg
     1921 aaacctaccg cgaaggatcc gaggttatct atcgtcctga cccgacgatc ccgcttcgtg
     1981 aactgccggc cgagaactac atgaagaccg ttgattactg gtataccaat ctcaagttgg
     2041 acaaaaccat cgaccttcaa ggcggcagga cactctccgc gtacatggat gtgacgaacc
     2101 ttttgaacac gaaatacatg agttcagggg gaatggcgag ttacaacgac tacctgaact
     2161 acgtgtttaa ccgccggcag gcgggggaga agaacctgcg ggtgtatgat ccctcgacgg
     2221 aagaggccct tacaagaccc tacaaagatg ccgggggcaa ctggaaggca cccatcagcc
     2281 cgcgcacgga atggctgctt tacccgacag cacgagtcgt gcggttcgga ctgagatttg
     2341 acttctaggc gagcaaacga gacacgagga aaaggcctgg ccgaaaagta ttcggccagg
     2401 ccttttccat actcactctg atccgtcggc ggcggcgacg gctcgaaggt aagcggcgcg
     2461 tttcaaccag tagtctgccc gtgcgtcgat gcggctgtcc gtggctttct ggcggagcac
     2521 ctgcgcttcc agaaggtcgg agaacgtgac aagcccgttc cggtaccgat ccgacacctc
     2581 ggccaggttc acgtccgcct gatctacggc ctcgttcgcc acctcattgg agcgccaggc
     2641 cgccgtcagg tccgtccacg cgttctcgat ttgcaagtcc agcaactcgc gcgtctcagc
     2701 aagccgattt tcggcaatct ctacccgcct gtgttggctg gccgttgtgt gcctcccctc
     2761 ccacaaaccg gatattggaa cgtttaccat tcccatcagc agaccgttgg tctggctgat
     2821 cattccgcgg atatcctgcc gcaaccctat tgcaccgacg gaaaccgtgg gtagcatccc
     2881 cccacgcgtg agggagttct gaagctgctc agcgcgaatg gcgcgggaga gcagacgaat
     2941 ttcgggacgt cgatcgagtg ctccggctcg ctcctgcgca agaatagatg ggtcgtcagg
     3001 acacgccaga gtgtcgacaa ggtgaagcgc accgccctcc ggcaacccga tatggcggcg
     3061 cagatcccgc gtcgccaacg ccagaccgtt ttcgagtcgt tgacgatcga cgctcgcttc
     3121 agaacgcttg agtcggactt tgagctgatc attgcgagtc aagagccccg cctgcacggc
     3181 atcggtgacc tgtttgtcca gcgcctctag cagtgcctcg tacgcgtcga gcgtccgaag
     3241 cttttcccga agggcaacga gccgccagta cttctcctcc gtctgcgcca gtacatcgcg
     3301 cttggagatt tccgccctgt ctttcgcaac gtggaccccg aggtcagcga ggcggtttcc
     3361 gctcacgatc cgcccgcctg caaacagcgg ttgaattact gccgccgaag ccacgaaacc
     3421 gcggcccgcg atcgccattt ccccgctcgg aaagtatgcg aactgagtgg ccgtggggat
     3481 ggttgcagga ttgccattgt acacagggag gttgcccccg ctcgtatgta tccttgccag
     3541 cggatccacc gcgaccgccc cggtggcgga taccgatatc tgagggaagt atttcgtgta
     3601 ggctgctcgc cgagtttcgc gggccgcttc tatttcgagg ttcgattccc tgatctgggc
     3661 gttgttctgg agggcgagtt ccttgcattc ctgaagcgtg agcggtgttt cgtcagcgct
     3721 cgccggggag actgccagag caagcccgag aagcaatccc gtcaccacag gagcctttgg
     3781 cgaaggctcg tgccggcggt cttccctgcc gaccacaagc gcgtacgtca ccggcaaaac
     3841 gaataatgtc agcaccatcg cagtaagcaa tccgaagaat gtcacagttc cgagagggcc
     3901 ccacaacgtg gaccggctga gaatcatcgg caccactccg accgccgcgg cagtcgatgt
     3961 aaggtagatc gggcgcatgc gccgcttgcc ggcggctcgt gcggcctcca tcgcgctcag
     4021 tccttgcttc acgcggagtt cttctgcata ggtaaccagg ataatcccgt tgcggacggc
     4081 gatgcccatc agtccaatta tcccaacgaa ggcagtgaac ccgaagggat accccatgat
     4141 cactaggcca agaaaagcac ccatgagggt cagcggcatc gaaagcatca cgacaagggt
     4201 ggtgaggaag cgttggaact ggcacagcac cacgaggaaa atgagtccaa tgcttaccaa
     4261 catcgaaatc gtcatcgggg agaagatctg cgtggaaagc tctttctcac caccgtaact
     4321 cacacggatg cctggcaggt cgagcccaga gacgtatttt tcaattttct cttgcatttc
     4381 cgaagccagc gcgtcctttt ttatgtcgat gaagaccgtg agggtgcgaa caccattccg
     4441 acggaccatg gcgccctcat gccacgccgg tctcaacgaa gcgatttgct ccagtggcac
     4501 ggtcgcgccg agaatcgaag aaacgtattg ctggcgcagc ccctcaacgc ttccgcgctc
     4561 tccagagtcg tcccgcacaa gaacacgcac ggggtaatca ccctcccaga tcgttcctac
     4621 ccggtacgcc tgtgtgccca gagcgagcga cgcctggagc atggcggggg gtacgcccag
     4681 acgggcgcac gcgtccgcgt ccggcataac gtcaacagat tgcagcgctt cttcccagtc
     4741 gttgtgcacc caggttactt cggcaattcc gcgtgcgtac gactctatct tcgccccggt
     4801 ggttttgagc gcttccgcgt cgttgcccga tagacggact tccacgggaa aaccgacctg
     4861 gaattcgagt tgcttccagc gcacccagca ctccgggaag gccccccgca gccgttcttc
     4921 actttcgcgg agaacgtcca gcgtcgcatc ggcgtcaatg gtgttgacga ggagttgccc
     4981 gaaattccgc ctcggtatgt agggcgcata cagcgtgtgg aaacgaggcg cgctttgccc
     5041 gacgaatgag gtaacgttga ccacccgctt atccgcaagg agttcggcct cgagccgccg
     5101 catgactgcg tccgtttgct tcagactgcg gccagtgggc aaatacacct ctacggcaaa
     5161 ctgatcacgg tccaccttgg ggaacatctg ctgcggaatg cgaagcgcga tcacgaccgc
     5221 gatgagaacc gagccaatcc cgcccgctac ggtgagccat ggatgacgga ataccgcatc
     5281 cagagccctg tcgaacacgc attggatccg ctcgagcatg gatcgctggc cgctccgttt
     5341 gcgtaacccc ctgcgaacga actgagagtt catgattgga aggagcagga gtgcgatcag
     5401 caggctgacg gaaagtgcca ctgcgatcgt cacgggcaag cttccgagga attccgcagc
     5461 aaccccggtc aggaaccgcg gcatgggaac gtaagacaga atgatcgcga tcgttgccgt
     5521 gaaaaccggc accgcgagat catgcgcgct catccaggca gctgaccagg catccatgcc
     5581 gcgatcaagc ttgtccacgt ggtcatcaat aaccacaatg gcgttatcta cgatcattcc
     5641 cagcaccaca ataagcccgg cgagcgagac ggtttgaagc tcaactccca gcgcgttcag
     5701 gatggcaaga gtgatcgcga tggacacggg gatagtaatc gcggcgactg cagccacacg
     5761 tacgggcaga agcaacattg tcacgagtat caccgaggca atggccagtc caaaatcgcg
     5821 gaggaagtgg ttgaccgatg tgcgcaccac tttgggctgg tcgacgacgc gcgagatcgt
     5881 cactcccggt ggtaactcgt gcaatgtctc ttcgagcgca cgatccacct cgtcgccgaa
     5941 ccgggttatg tcgttgcccg gctgcatttc gatggacagc acaagcgcag ttttgccgtt
     6001 gaagcggacg tacgcgtcgt catggccata ttcccgttta atctccgcca catccttcaa
     6061 acgcacgtgc tggcctgtcg ggagggacag gaggatcgtt tctgcaagct cattctcgga
     6121 gcgcagcacc ttcctcacgt agatgggacg ttccagttca tccccgtcca gtcgggcgta
     6181 ggctggaagc ccgcccaaac cctgtagaga ggcccacaga gtagctggcc gaataccgta
     6241 acgcgcaaca cggtcccggg agatgttgat gctgatgact tcgtcctgct caccgattac
     6301 acgcagtttg gcagttgcct cgatccggcg cagatgggat tcgagcactt tcatgtattc
     6361 gcgcaaatcg cgcgggcttt tcccttccgc caccagagtg aaaagcaggg cgctggtgtc
     6421 cccgaactcg ttgttgccga caagagcgag caccccaccc ggaagtttct gggccttcag
     6481 ttcgttgagc ccgtgccgga gttttgccca gaaggcggcc gtttggatcc cgtcaagatc
     6541 ctctttcacc tcaacgaaca ctacaagttg gccgtctttg gagaccgagt acgtcttcgc
     6601 cttgttgacc tcgttgaagc tgaacagaaa atcctccacc ggccgggcaa gttggtcctc
     6661 gacctcttcg gaggtggcac ccggcataac gccgatgacc agtccctggc ggatggtaaa
     6721 ggccggaaac tcctgccgct gcatcgtgaa aaaggaatag actcccgcca ccacgcagac
     6781 cgcgaccacc gtcactgtga ttcgccactg gcgcatggct ctttcggtca gactcgttgg
     6841 atccatggac tattccccgt tcgcgtgagg cgcgagaacc cgcaccgtca ttccgtcggc
     6901 cagcatgggc gtgcccgata ccacgagcga ttcaccggcg cgaacgcccg ccgtcaccat
     6961 cgttccctcc ccgacaaacc ctcctaccct caccgggcgg cgttggagcc tccggtctgc
     7021 ggcgacaacg tacacatagg ggttgccgac ctcatcaact cggattgctt cgggtgggat
     7081 aacgaggttc tccacttccc cgtcgacctt gatgtacacg tccgcgatca tgccgaggcg
     7141 aagctctccg gtagggttcg ctacagccac cttcaggtca tacgttctgg taagcgggtt
     7201 cgccacgacg gagatctccc gcacacttcc ctcgacggtt ttctgcaggg ccggaatcac
     7261 cacacgtgca ctcatgcctc gccggatctt cgatacctgc gtctctggca ccggagcagt
     7321 tgccagaagg gttttcgcct gaaccagcgt gaaagcggga agcacaggtg aggcactggc
     7381 accgggttcg acgttgcggt cggctatcac gccgttcacc ggcgagcgaa gactcgtatc
     7441 atcgagattc ttccgcgcga tggacgccgc cagccgcgcc tgctgacggg ctgtttcgac
     7501 ctcgaccatt ttcacgtcag ccagagttcc gttgcggtgc atgggctcga ggcggcggta
     7561 tgcatcctcc gcctggttca ggttcgcctc tgccatcgcg agcgcgtcct cgtagctccg
     7621 cgacacaagc ttggcgagca catcgccttt tttcacctcc tgaccttcct ccaccatcac
     7681 ctgctcgacg gttccaaggg tggcgaagct gagggcgatg gatcgttcag ccgcgagcgt
     7741 accgctcaaa tgaagctcgc gcccgtttcc gacgcgtgtc actggcttga cgaatacgcc
     7801 gggttcgacg gaagtggttt tttgaacctt gttgccgcac gctgcgaaga agggactaag
     7861 gcagagagtg gggaggctcg cgacaatccc aagtaaaacg cgcttcgggg gagttgtcca
     7921 taccatcgtt agactccttc tcactattgg aacaaaccga tgaaacgttc gaatcttcgt
     7981 aaaaaaaggg gcttctgaag cctgttcgcc gcccggctca ccggcagaga ccgtcgaaaa
     8041 gaatctccag catctcgtca atagcttctt cgatcgacaa ggagtcgcta agtccggcaa
     8101 gaccgttttc aacgccttga agcgccgcga tgagaaggag cggcccattg cgagttcgga
     8161 gtacccggaa aactccctgc cgaccaccgt cctccaagat ttcttcgata agctttccct
     8221 cttcgtggaa aaactgctcg cgcgcatggg cagttttcgg aagaaattcg cgaacctcgt
     8281 gacgtgcatc gccgacgact tcgttcagta tttcactcat cgccctgaac cgggtgcgaa
     8341 tgacctccgc gaggcgggcc ttcgggtcgg tttctgatgc ggcggcggct cgcattttcg
     8401 aaagcagaac cccactttcc gcgcgaacta cctcggcaaa taccgcttct ttgtctggga
     8461 aatagtgata tatcgcagtt ttgctcaggc cgcaggcctt cccgatgtca tcgagcgtgg
     8521 ctttgcggta gccataccgg aggaagacag agcgagcctt ctcgcggatt tccgttttcc
     8581 tgtccttttc acccgtgtcc gcaggcgcgc gtgtgccgtc acccacgccg ctcatttctg
     8641 tgtcattcat tggattgcct cccggcaacg gtgacttcaa gcagctcccg ctgaccaatt
     8701 gaccatttga ataatatagc taaacagtca aacacgcaac aagtaaccag aatctcccgg
     8761 gaacagatgc gtggatgcat tcgagcggcc gcgaaagcag aagctacccg tcatcaacgt
     8821 gttcgtggtg tcaggaaact gagcgcactg accaaatcca tgagagccgc gttgtatttg
     8881 ttggaaggaa atcagccgcg ttccagcatg aggcaaggct gcgaacagcc tggcctgccg
     8941 gcaagggctc ccgctgccac tcggctattt cctgcaaccg ggctggatca ggaaggacca
     9001 tccgtccgcc gaaagcgagc ctgaattcgt cgcaagcccg gtgcgagcac gagatagacc
     9061 agcacggctg ccggcggcat gaatgcgcac cacgcgacaa ttgcctgtaa ccctgtgacc
     9121 cacagcaagc ccatggagcc ctggaaatct gaacggaaca tggccgccat ctccgaggcg
     9181 gagaatggga tgggggccgc gtggaaaatc cattccccga agcggatgaa agggatgagg
     9241 agcagaagct ggaggggaaa catgacgtag ttgacgatct gaatcgccgg catgttgagg
     9301 cggaagagga atgccgcgag agtgcacaaa atggtggtgc tgcccagcac ggggaacacg
     9361 ccgagcgcgc cacctacggc catgctgagc gcgatctttt ccggggtcac tccctgcttc
     9421 agcaagtcca tgatcggcga gatcaccctt cgccggacga agcttcctgc acccatcgac
     9481 accctcgcga gacaaagttc gaaccacggc ccggatacac tatgatacgc aggaaatcgc
     9541 gtgaaggaac ttcgcggact ctcgctgacg gtccgggatt gtccctgcgc cagattggat
     9601 gtaggtatgg taccggagac ctcaaaagcc caccgccaat ggagaagcct gaggtgagaa
     9661 ccgccggaaa ccccaatctg ctcttcatca tgacggacca acaacgcagg gacgcgcttt
     9721 cgtgcatggg aagccggttc gttcacacac cgtccctgga tcggcttgcc gtggagggcg
     9781 tgcgcttcac gaacgccatc actcccagcc cgatctgcgt tccagccaga gcatcggtgc
     9841 tcaccggttt gccaatccac cacacgggat gcattaccaa ccctgacagg ttgagaaccc
     9901 ctgcctccga tttgacgaca ttcgacgacg cactcaccgc acacggctat acggcagaat
     9961 acatcggcaa atggcatgct ccagaagcct tgctcagacc ttactcgcgg ctcccgacat
    10021 ctgtttccct cgataccttc tatcgcggct atctggctga aaaagggtat ggttccccgc
    10081 accagggtgc atcccacccg tacatctcgc cactgagcgg gcaaccgtac gaccctgacc
    10141 cgatggacca cgtgtaccgt tctctcaatc tgggccgcgg tcactcccgc ccggagccgg
    10201 ggctcccttt cggacgggat tcggtgcgtg ctgagcactc agtctccgcc atgattgccg
    10261 atgagacaat acggagcatc gagaagctca aatacgtccc tttctcgatc acaagttctt
    10321 tccttttccc ccacgatccg ctcatcgttc cgcgtcccta ttgcgattca gttcgggcag
    10381 ccgatatggc cacaccggag acgtttgacg acgcgcggat gaacacgccg tacaaggatt
    10441 tcgtatggca gatggacgac ctcgaacgca gacacgtgca tctgttgatg gcgcggtact
    10501 atgcggcggt gcaggaagtg gatcatcaca ttggccgcat tctcgacgcg ttggacacct
    10561 cgggcctcac cgacaacacg cttgtcgtgt tcacctcaga ccacggcgag atgcttggag
    10621 accacgggct gatgacgaag ttcgttcact accgcgaagc agtcggcgtg ccgcttctga
    10681 tgcgcatgcc cggacgcatt ccggagggaa gcaccgtgca agcgccggtt tgcctgaccg
    10741 atctcttcgc aacaatatcg gattatctgg gcgtcacgcc gcccgaccgt tgggggcgaa
    10801 gtctgcagtc atggatcgcc ggcgacgctc ctgaacccga tccaatagtc ttctcgcagt
    10861 tcgagcaccg gaacgtcatg gcgcagactt gcgcatggaa gtatgtgtgg agcaaccatg
    10921 ctgacgaggt tgacatgctg ttcgatctta ctcgcgaccc tggagaagtc aataacctcc
    10981 acggcgcgaa cccggatcgt gcgcggtacc tccaacaggc agccgccatg aaagaacggc
    11041 tggcggaatg gatggaggaa acggaacatc cgtggctgga gcagttagcg gccacaccgg
    11101 tgagataaac gctcgtgaga cagggattga aacccacgaa ccgataaggt gccttcagga
    11161 agacggcagt aaacgggaca cgctctccgc gactgcctct gcgcacgctt gttttcctgc
    11221 ctcagaaagg tgaagctgat cagggccaag atactccgaa gtgttgctcc acgccacggc
    11281 gtgcagatcg ttgaccggga tccccctttc ctccatcagt tcaagggccg cacgattgta
    11341 ccgatcaatc tcctcgtttc gccaggacca cgcatcggcg cgaaacgggc gttcagggtg
    11401 caccggtgtc gtcgtcgccc atatgatttt gtccgtcagc cggctcagcg catcaagaat
    11461 gtgcgccaga ttcgcccggt acatgtcgat cgggatctgg attgggtgcc gttgcggatt
    11521 gtggcctgcg tcatggagac cattgttcca gtgcacgatg tcgggcttcc ccagctcgtt
    11581 ggcccaacgt gcgagggatg aaagcgtgta aaggctgtac tgacagttgt ccgcggggcc
    11641 caggacctcg gccctgcctt ccagcagccg cgccaccagt ggctggtagc tcatgcggat
    11701 tgaatctccc agcagcatta ctttcaccgt ccctgccatc gttgtccctt tccgtggcgc
    11761 cgatgtgttc tcaaaatcag catcaaaccg ccccgagtac ccgcatcaga gctggcgtgt
    11821 tctaccggca ttgcaacctc ccggctggcg accggtgccc cattcctgat gtttccaaac
    11881 cacctcgttc acgtctgacg aaacgtgacg gtgcgagatc tgtagcacgc gtccgcgccg
    11941 cgcggtatca cttcaccaca tcggatcacc gagaatgcta tgggaggtat cctcaggagc
    12001 ggccagcaaa tcatacaacc gtaccagttt catcgcgcgg ggcaggttgc tccctttgat
    12061 ttccatctcc acggacaggc gcgtcgagcg cgggtaccgc aggtacgtct cccgcacggt
    12121 cggaccggat tcctccctgc gctctatgac gaggccgttg tccgtccact ccgctttgac
    12181 tttggcgttg ttcccgtcaa gccacaccac gtcgcgtctg cgcccatcgg taatcaccgt
    12241 aacctcaggc ccgccgagtg cagacaacac aacggccgtg tccgtctgca tgatggtcag
    12301 gtttcgaatg tggtcgggca tgcgggggcg ctcaggacgc gcgccacccg gggcgcttcc
    12361 atcacgcggc ctgccgcccg gaaaaccacc tcccagatcc gtaccgcccg gcactccgcg
    12421 cgccggtctt ctctggcttc ccggcattcc acgagcgtcg ccccctcgca cactttgatt
    12481 ctgctcccgc gacgcagttt cattgaatac ccagcgaccg gacagatcaa tgtcgatcgg
    12541 gggcgtgcgg gttgaagcgc gcgacgaagt ccacgcaagg aaacagacaa tcacgacggc
    12601 gcccgctcgg agcgcatgta aagaggagca acgcattcgt tcacctccat cctgagtggt
    12661 cgaacaggag tgatctgtac ctctgaacga atgcgccggt tgttttggta tctgcccgaa
    12721 gggctgtttg ctgcgagaca cccacgcttt catgactgcg ctgttcggaa ctcaaacagg
    12781 aaaccccgac ggatgggacg acatcctgtt cccggtcaac cgatataccg cggggcggtt
    12841 tccgaccata ccccgcgcca gtcttccgga gcaacatcca gcatcggcag gccgcgcttc
    12901 cggccttcct ccattatgag acgtcgtctt tcgcccatgt ccctgtacaa cctgtcccag
    12961 cggta
//
LOCUS       DAOTNG010000050        12943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4652_length_12943_cov_5.336204, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000050 DAOTNG010000000
VERSION     DAOTNG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4652_length_12943_cov_5.336204"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="P5179_03820"
     CDS             <1..298
                     /locus_tag="P5179_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRS94379.1"
                     /translation="CLGQEFVSRAAATFFWTTIPARMEWRYDRAAHKVIAVDAGHVCQ
                     NLYLACTAMGAGACAIAAYDQDACDRLLSVDGDEEFTVYIATVGKRRRAQSAAS"
     gene            357..1187
                     /locus_tag="P5179_03825"
     CDS             357..1187
                     /locus_tag="P5179_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRS94380.1"
                     /translation="MVGTRINAAARSYSMRPSRVLRVIRAGGVASCLKVNSSDARIVE
                     VMASSGCDAVWVCTEHTPADYANIENQVRAAKVFDVDLVVRCPRGSYSDHIRGLEADA
                     AAVMVPHVMSVHDARNIVNMTKFPPLGKRAVDGGNADGLYTRIPFTDYVQQANTERFV
                     IFQIEDPEPLEELDAIAALPGVDMLFFGPGDYSTAIGMPGKIWDPKIMAVGERIATAA
                     RNAGKMAGTVSVPAKIRALVDMGYNFLNIGSDVGALTEFSNQRVDAFMDSLAGLKANG
                     "
     gene            1232..3442
                     /locus_tag="P5179_03830"
     CDS             1232..3442
                     /locus_tag="P5179_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94381.1"
                     /translation="MDVFAPSAFLDPPSKFRPLQIVHGFDRFLEHPGDLIGEEGIGRR
                     LDQLRRVGIGGVVSNVGGGFGEYLLEERQWEVWVSGMQQAHERGMVLWWYDEKGYPSG
                     TAGGIVTRANPSLCAVGLACYSLSATGPGRFQFVLPASCLKFEVAFASGDFDRVGMDG
                     VIGLTSSTDENGCLYWEVPEGDWTVLYLAQRYMYEGTHAAANVCEFKHYVNLLEPEAT
                     RRFLAVTHDEYFRRTPPGLWKNVRAVFTDEPSLMTGYAGPLPQRFQGNVPVIDKPLFR
                     DRPVAVPWSQGLVAAFTERKGYDPKPRLWCLFCGEHEDARAFRQDFYEIVTELYTNGF
                     YRQVRNWCDRHNIAFSGHVMAEEWLGGHVEYHGSLFAPVREMSLPGIDMLDSDPVSIL
                     EGHGFMTAKQVASIAHLTGAQQVHSECSDWSQRNNGRGATLEQRYGQGGLLYVLGVNQ
                     VTAYWSWDDIGDEAYRTYNDYMGRLGMLLRGGVHRCDVAVLYPIRTAWLDFLPHASYE
                     ADNAGQKVVSTAGARERFNAVGAAYADTVRDLLRSQIDLDIIDEQALTEGIIGAGALH
                     VAGESYRVIVLPQCEAISLSAAEALAAFGRSGGLVVSIGAPPRLVESARATAKVHEGM
                     EQLFGARGNGRILKKEEVVPYLRSARCATLELAEANPSILCTERSLDGRGLCMIVNAS
                     GEPQLIRPRSRLREPLDLYRPLDGSIAPFRAGAELHLGSFESVFLVAQAPSPPV"
     gene            complement(3459..5669)
                     /locus_tag="P5179_03835"
     CDS             complement(3459..5669)
                     /locus_tag="P5179_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS94382.1"
                     /translation="MQDRLAAFRDQAYARGFTSHHSPKEKNYSYAFFDRYADLSLPER
                     QARSLAYALVNEPVLIHPLSRIPGQIYQACAGSACPELSGSTEHPGWVDFSIGQSAER
                     RVAEEFPDGEYYGKYFTQGGYPGHICWDFGRMLDLGIEGMLGLCERGQRQTTAPRSRE
                     FYHCVAIVLQGLLSWVELHVSAIENLMREEVDPVRRKELREMADVCRRVPRNPSTTFR
                     EAVQTFWFQHLAVMYENPFGGNGPGRLDYYLWPFLERDLASGRITLDEARELVTELLI
                     KLHERIAPADGWVEALPVGGRKPDGSLAINPLSSMIIEIISELPQTHPSVYVRLPEDA
                     PDDFVDLTVRYLLRAGNRAQVYGDDAVIEALHRSGKVAVEDARHWTAGGCMEVSPQGC
                     NCDLLFSFAHNVARTFELVINGGCLMQTGERVVDHPKTLADYTSFEELYTDFEAELAR
                     ELGILARRLDIYLEEYARLRPSFLLSSMTHDCLEKGRSINDGGARYTNYGGSGVGIPN
                     VGDSLYAIQRAVFLDRRVTAAELLNALRTDFVEYEALQAYLRSLPKYGAGDEAADAMT
                     DRVFCTFADLLASHTTRHGGTLVPIILGFVWVVDFGRQVGATPDGRNAGRPLAHGLSP
                     QSGSAVKGITGAIRSATSLSLDKAGGGGAMMWDLDPSWAAPSVVKPLLKTFVDRGGHI
                     FQGNVISVETLRKAQQNPDDYRDLIVRVAGWSARFGTLSPATQEEIITRYKYAG"
     gene            complement(5887..5959)
                     /locus_tag="P5179_03840"
     tRNA            complement(5887..5959)
                     /locus_tag="P5179_03840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5924..5926),aa:Lys,seq:ttt)
     gene            6068..6655
                     /locus_tag="P5179_03845"
     CDS             6068..6655
                     /locus_tag="P5179_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRS94383.1"
                     /translation="MIRRMYDWVLSWAEHPAGVVALAVLAFSESSFFPIPPDVLLIAL
                     AMGKPKRAFWFAAVCTMGSVAGAMFGYLIGSVFWNAVSPYFFSYVPGFTPRQFEIVRG
                     MYEQYNFWIVFTAAFTPIPYKVITVTAGVFGINFPLFLVGSIIGRAGRFFLVGAMIWR
                     FGPPIKTFIDRYFNALAVAFTVLLVGGFVAIKYAF"
     gene            6689..7765
                     /gene="galT"
                     /locus_tag="P5179_03850"
     CDS             6689..7765
                     /gene="galT"
                     /locus_tag="P5179_03850"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRS94384.1"
                     /translation="MSGFRKNSVAYSPKQWEMRWHPIRKEWVGFAAHRNTRPWQGEQA
                     PQPPPPPPFDPTCYLCPGNTRNRGERNPDYSGTFVFDNDFPPFGPDAPETTTEHGLYK
                     AMPAYGRCRVVCFHPRHDLTLAEMNREDIRRVVETWQAEHRMLAKDSRINHVLIFENK
                     GSVVGVSNPHPHGQIYATEFVPPVTVTELASSREFYAEKRRTLVGAILEQELRDEVRV
                     IAQNDTSVAFIPYFARYAHETYIVPRRPMPSVADLTPDEADDFTEILHTMLVKFDNLY
                     GISFPYVMAFHQAPTDGAAHPEYHFHVEFHPPLRYPGVLKYLAGAETGAGVFLNDTSP
                     EEKAKEFRAAGNIHYKATGGQKTA"
     gene            complement(7835..8080)
                     /locus_tag="P5179_03855"
     CDS             complement(7835..8080)
                     /locus_tag="P5179_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HRS94385.1"
                     /translation="MAKMITCSRTGKVVPQMEKPPMRGKIGQEIWENVSQEAWDEWTR
                     REVMLINEYGLNLADPEDRKVLYENMREFFNLPGKDK"
     gene            8195..8992
                     /locus_tag="P5179_03860"
     CDS             8195..8992
                     /locus_tag="P5179_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94386.1"
                     /translation="MNTEHECLIRWEEVHKSFGANHVLRGVTLQVCQGETLVIIGRSG
                     CGKSVLLKTLVGLISPDSGRIWVKDRQIVGDPSAMAEAQRLCGMVFQGAALFDSLSVA
                     ENLALPYWENTDLSKGEIEERIRSLLALVGLRDVEQLMPAELSGGMRKRVSLARALAD
                     EPEIMLYDEPTTGLDPIMADAINELIRSTQEKVKLTSIVVTHDLSTVRKVANHVAMLH
                     EGRIVFHGTPDELFASADPVVTQFIQGRADARVEVPRPELREHEIDE"
     gene            8985..10748
                     /locus_tag="P5179_03865"
     CDS             8985..10748
                     /locus_tag="P5179_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94387.1"
                     /translation="MSKPNREERRSASSSWVSWLVEMARAERLWFGGTVLFSVMGALT
                     GALATWLMVPLAAVLFPESTGADAIHNPSRGLYPVSAGAWLTSATASITPLSVALSFV
                     VVFTVKNLCEFGSRVGCDALVAKVEFRLRNSLWRQYLACGRASGRSFSGARFIHELLA
                     GAGEPAAAVGAIPVRWIGDPVAAVALLGTMAIISPHLTSAMLLLLPPAWWATRKGFRH
                     IGNRTETRLAARGRLGARLSELASFRPVLASHAAMDWALINTGRHEQSVIRAALSWSR
                     VARLIPLTAETLGAFVGAAVILLVAKGIRAGTLSGGEFLTFLTAFFLLLPVAKRLAAL
                     SGDMRTSRSAWERLASLLPADARAEVAVPPSETFRAPAITLRKLRASIPGGNRVLDIG
                     YAEFPPGEVTAIVGPTGSGKSLLLEIIAGFGVPDEGTVCWNERPISSPHVVPERPPFG
                     YVPQDRWTISGTVGENVTLGRPVSETALTTTLQALGLDLDVQTELGQDGAPLSGGERQ
                     RLALARALLGEPPLLLLDEPTSALDAASEERVIALLRSLKGRTTVLVATHSTAVISAA
                     DWVFHLPFQSATTALHKETVR"
     gene            10748..>12943
                     /locus_tag="P5179_03870"
     CDS             10748..>12943
                     /locus_tag="P5179_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94388.1"
                     /translation="MRVKMLAAGCVFALSWCTSPRADVTSRYAAQFEEALASMAMSPA
                     DVAFRTDHVERDSFRLRRTDVFLLHPLSFADSLASFSARAGEFGGPSPYVHLAADWLD
                     DPLPPLCEPAARVLVRDTTRCARAFNRLAGAIEHAARIVQESQSVFSPAERESLSTWV
                     DRVLSEGDPDVSGEQVDLFAWKAEELAELQCSRANLELAKRWSGREILRAASCVASAA
                     WELREALMEAKNRVPMPLPVKTWHTTCGRMTVGSDAQDNYTGDFALIVDQSGDDTYRV
                     RPPGSGGGQVQVVIDISGNDVYFGSIAGSTFGIAISLDVSGNDTYIGEAWTQGSARFG
                     AAILWDERGNDTYYANRAVQGTGMFGLGLLVDVDGNDLYRSGTYGQGFGATGGIGILD
                     DRKGNDSYYAGGATTDVLRFDDHYLTMAQGVGSGIRPVGSGGFGFLIDRKGNDVYNAD
                     VYGQGVGYWLGGGGLLDGEGHDRYVAHQYAQGAGIHLSSGALLDFRGDDVYVINGVGQ
                     GCGHDLSVGILFDETGDDAFTVEGLALGAGNANGISVFANVSGRDAYIARREDVMGYS
                     DRRREYGMIGVMLDLGGEDRYAAPLGANDRWWHHSTFGAGVDLGSVSPSSDTATAADL
                     SLTEGGHRQKIAATAESLFVQASNPFSALQYLVEPAENRLADMVGESVTAFWAGKLAS
                     ESARERWALVRIHQKLFARGDLSSVPMLIDSLESPSGAARRMAAHLLGFS"
BASE COUNT         2565 a         3712 c         3961 g         2705 t
ORIGIN      
        1 gtgcctcggg caagagttcg tttcgagagc cgccgcgact tttttctgga caacaattcc
       61 cgctcgcatg gagtggcgtt atgaccgcgc agcacacaag gtcatcgcgg ttgacgcagg
      121 gcacgtgtgc cagaacctgt acctggcgtg cacggccatg ggagctggcg catgtgcgat
      181 tgctgcgtac gatcaggacg cctgcgaccg tttgctcagc gttgacggag acgaggaatt
      241 cacggtgtac atcgccaccg tgggcaaaag aagacgcgcg cagagcgccg catcatagtg
      301 aaaagcggtt ttttcagtcc ggcgtcccgt ctggcagaac aatggaggtc agtaatatgg
      361 tgggaactcg catcaatgcg gcggcgcgca gttattcaat gcgtcccagc agggttcttc
      421 gggtaattcg agcaggcggc gttgcatctt gtctcaaggt caactcgtca gacgcacgga
      481 ttgtcgaggt aatggcatcg tccgggtgcg atgcggtctg ggtctgcacg gaacacacgc
      541 cggctgacta tgcgaatatc gagaaccagg ttcgcgcggc gaaggtgttc gatgttgatc
      601 tggtggtccg gtgtccccgc ggaagctata gcgaccacat tcgtggcctc gaagcagatg
      661 ctgcggcggt gatggtacct catgtcatga gcgttcatga cgcccgcaac atcgtgaaca
      721 tgacgaagtt ccctcctctg ggcaagcgcg cggttgacgg tgggaacgcc gacggcctgt
      781 acaccagaat ccctttcaca gattacgtgc aacaggccaa caccgagcgg ttcgtcattt
      841 tccagattga ggaccccgag ccgctggaag aactcgacgc catcgccgcg ctgcccggag
      901 tggacatgtt gttcttcgga ccgggcgatt attcaactgc catcgggatg ccgggcaaaa
      961 tctgggatcc caagatcatg gcggtaggcg aaagaatcgc gactgcagcc cggaacgcag
     1021 gcaaaatggc gggaacggta tcggtgcctg cgaaaatccg cgcgctggtg gatatggggt
     1081 acaacttcct caacatcggc agcgacgtgg gagctctgac cgagttctca aatcagcgcg
     1141 tggatgcatt tatggattcg cttgctggcc tgaaggccaa cgggtgatat agtctgagac
     1201 cgcgcgtctt ttcttgatgg agaccgagaa catggacgta ttcgcaccct ccgcgttcct
     1261 tgatccgcca tccaaattca gaccccttca gatcgtccac gggtttgacc gcttcctcga
     1321 gcacccgggt gatctgatcg gcgaggaggg gattggacgc cgtctggatc agttgcgccg
     1381 agtcgggatc ggcggcgttg tgagcaatgt gggaggagga tttggcgaat acctgctgga
     1441 ggaacgccag tgggaggtgt gggtttccgg catgcagcag gcacacgaac gtgggatggt
     1501 actctggtgg tacgatgaaa aagggtatcc aagtggcact gcgggcggaa ttgtcacgag
     1561 agcgaatcca tcgctctgcg cagtcggcct cgcgtgttac tccctttcgg caaccggacc
     1621 gggacgtttc caattcgttc tgccggcgag ctgtctgaag tttgaagtgg cgtttgccag
     1681 cggagacttc gacagggtcg gaatggacgg ggtaatcggt ctcacaagct ccaccgacga
     1741 gaacggatgt ctttactggg aagtgcccga aggcgactgg acggttctct acctcgcgca
     1801 acgttacatg tatgaaggca cccacgcagc ggccaatgtc tgcgagttca agcattacgt
     1861 taatctgctg gagccggagg cgacgcgccg ctttctggcg gtcacccacg atgagtattt
     1921 ccggcgtact ccgcccggtc tgtggaagaa tgttcgcgcg gttttcactg acgaaccgtc
     1981 gttgatgacg gggtacgcag gtccgcttcc gcaacggttc caaggaaatg taccggtaat
     2041 cgacaagcct ctctttcgag accgtccggt ggcggttccc tggtctcaag gtttggttgc
     2101 cgcgttcact gagcgcaaag ggtacgatcc gaagcctcgt ctctggtgtc ttttctgtgg
     2161 tgaacatgaa gacgcgcggg cgttccggca ggatttctac gaaatcgtaa ccgaactcta
     2221 caccaacgga ttctaccgcc aggttcggaa ctggtgtgac cggcacaaca ttgcattttc
     2281 cgggcatgta atggcagagg aatggctcgg aggccacgtg gagtaccacg gaagcctgtt
     2341 cgcgcccgtc cgcgaaatga gtctcccggg aatcgacatg ctggattcag accccgtgtc
     2401 gatcctcgag ggacacgggt tcatgactgc caagcaggtc gcttcgattg cccatctcac
     2461 cggtgcgcag caggtgcatt cggaatgttc tgactggtcc cagcgcaaca acggtcgtgg
     2521 cgccaccctg gaacagcggt acgggcaggg aggcttgctc tacgtgctcg gggtgaacca
     2581 ggttaccgcg tactggagct gggacgacat cggcgatgag gcgtaccgga cgtacaatga
     2641 ctacatgggc cgccttggca tgttgctgcg cggtggtgtc catcgctgcg acgtggccgt
     2701 gctgtacccg atccgaacgg cgtggctgga tttcctgcca catgcatcct acgaggctga
     2761 taacgcgggt cagaaggtgg tgtccactgc gggcgcgcgt gagcggttca acgccgtcgg
     2821 agcggcatac gcggataccg tgcgggacct gctgcgcagt cagatcgacc ttgacatcat
     2881 tgacgagcag gcgctcaccg aaggcatcat cggtgcagga gcgttgcatg ttgcaggtga
     2941 atcctatcgc gtaatagtct tgccgcaatg tgaagccatc tcactttccg cagcggaggc
     3001 tcttgcggcg ttcgggcggt cgggagggct ggtagtaagc ataggggcac cgccacgact
     3061 cgtggaatcc gcccgcgcga cagcgaaggt tcacgagggt atggagcagc tgttcggtgc
     3121 cagggggaac ggacgaattc tcaagaagga agaggtggtt ccttacctgc ggtccgcgcg
     3181 ctgcgcaact ctggagctgg cagaggcgaa cccttccatt ctgtgcacag agagatcact
     3241 tgatggccgc gggttgtgca tgatcgtgaa cgcgagcgga gaaccccaac tcatcaggcc
     3301 gcgttcccga ctgagggagc ctcttgacct gtaccggccg ctggacgggt ctatcgcgcc
     3361 attccgtgcg ggcgctgaac tccatctcgg gagttttgaa agcgtttttc ttgttgcaca
     3421 ggcaccttca ccacccgtgt gaaaggaact gaccctggtc agccagcata cttgtaccgg
     3481 gtaatgattt cctcctgcgt ggcgggcgag agcgtcccaa agcgggcgga ccaccctgcg
     3541 acacggacga tcagatccct gtaatcatcc gggttctgct gggccttgcg gagagtttcc
     3601 acggagatga cgttgccctg aaaaatgtga ccgccccggt ccacgaaggt tttgagcagc
     3661 ggcttcacca cggatggcgc cgcccacgaa ggatccaggt cccacatcat ggcgccccct
     3721 ccccccgctt tgtcgagaga gagactggtg gccgaacgaa ttgcgcccgt gatccccttg
     3781 accgcggaac ccgactgcgg ggagaggccg tgtgccagtg gacggccggc gttgcggcca
     3841 tccggcgtag cccccacttg tctgccaaaa tccaccaccc atacaaagcc aaggatgatc
     3901 ggcacaagcg ttccgccgtg gcgcgtggta tggctggcca gaagatcggc aaacgtacaa
     3961 aagacccggt cggtcatcgc atctgcggcc tcgtcaccgg caccgtactt cggcagagaa
     4021 cggaggtatg cctgaagtgc ttcgtactcc acaaagtcgg tgcgcaatgc gttcaacaat
     4081 tccgccgccg tcacgcggcg gtccagaaaa acggctcgct gaatggcgta aaggctgtcg
     4141 cccacgttgg gaatgccgac gccgctgcca ccatagttcg tataccgcgc gcccccatcg
     4201 ttgattgagc ggcccttttc gaggcagtca tgagtcatgc tggaaaggag gaaggagggc
     4261 cgcagacgcg catactcctc cagatagata tcgagacggc gggcgagtat ccccagttca
     4321 cgtgcaagct ccgcctcgaa atctgtgtaa agctcctcaa aggatgtgta gtcagccagc
     4381 gttttcgggt ggtcgacgac acgttccccg gtctgcatca ggcagccacc gttgatgacg
     4441 agctcaaatg tgcgggcgac gttatgcgca aacgagaaga gcagatcgca gttgcacccc
     4501 tgcggtgata cttccatgca tccgccggca gtccagtgac gtgcgtcttc cacggcgact
     4561 ttgccgcttc tgtgaagcgc ttctatgacg gcatcgtcgc catacacctg cgctcggtta
     4621 cctgcgcgga gcaggtaacg gacggtgagg tcaacaaagt cgtccggcgc gtcttccggc
     4681 aggcgcacgt agacggaggg atgcgtctgc ggcaattcgg agataatctc gatgatcatg
     4741 gaggaaagcg gattgatggc aaggcttccg tccggtttcc tgcctcccac gggcaacgct
     4801 tccacccatc cgtcagctgg agcaatccgt tcgtgcagct tgatgagcaa ctcggtcaca
     4861 agctctctgg cttcgtcgag ggttattctg cctgacgcga ggtcgcgctc aaggaagggc
     4921 cacaggtaat agtcgagccg ccccggtcca ttccctccga aaggattctc atacatgacg
     4981 gcgaggtgtt gaaaccagaa cgtctgcacg gcttcccgga acgttgttga agggttgcgt
     5041 ggcacccgac ggcaaacgtc cgccatttcc cggagttcct tgcggcggac cgggtcgact
     5101 tcttctctca tcaggttttc gatggcgctg acatggagtt cgacccatga aagcaacccc
     5161 tgcaggacaa tggccacgca gtgatagaac tcgcgggatc gtggggctgt cgtctgtctc
     5221 tggccccgtt cgcacagacc cagcatgcct tcgatcccga ggtccagcat ccgcccgaaa
     5281 tcccagcata tgtgcccggg ataccctccc tgcgtgaagt atttcccgta gtactcgccg
     5341 tccgggaact cttccgcgac gcgacgctcc gcagattgcc cgatggagaa atcaacccag
     5401 cccggatgct ctgtgctgcc actgagttcc ggacacgcgg aaccagcaca cgcctgatag
     5461 atctgaccgg gaatgcgtga gagcggatga atcaggaccg gttcattcac cagtgcgtac
     5521 gcgagactgc gcgcctggcg ttccggaagc gaaaggtcgg catagcggtc gaagaaggcg
     5581 tacgagtagt tcttttcttt cgggctgtgg tgggaggtaa aaccgcgtgc gtaggcttgg
     5641 tccctgaatg ccgccaatcg gtcctgcata aggacctttt cctctcgttg gggatgtaca
     5701 cgcgggcaac gcatcagacg ggccgcgctc cgaacgaagt caatttaccg agtcacccgt
     5761 ctccacgcca aactcagggc agtgtaccga aagcatcggg gagaccgact ggcgtcgaga
     5821 acggccttgc actaaggaaa aagcagtgac tttgcgctga tcataagtca ctgcttacat
     5881 tagatatgag cccggcggga atcgaaccca cgaccaacag attaaaagtc tgctgctcta
     5941 ccgtctgagc tacgggccct gaaacaggca agatttttaa atttactcat ttgcgcgcgg
     6001 atgtgtcaac tacgctcaga acgtgcggca aggttggctt cccgatgcgc gggtcgggat
     6061 cgtatagatg attcgccgca tgtacgactg ggttctgagt tgggctgagc atcccgcagg
     6121 cgttgttgca ctggcggttc tcgccttctc tgaatcttca ttcttcccga ttcccccgga
     6181 tgtgcttctt atcgctcttg ccatgggcaa accgaaacgg gcattctggt ttgctgccgt
     6241 ctgtacgatg ggctccgtcg caggtgccat gttcgggtat ctgatcggca gtgtcttctg
     6301 gaatgcggtg agcccctatt tcttttccta cgttcccgga ttcacgccgc gtcagttcga
     6361 gatcgtcaga gggatgtacg agcagtacaa cttctggatt gtcttcactg ctgcattcac
     6421 tccgatcccc tacaaggtca tcaccgtcac ggccggtgta ttcggcatca atttcccgct
     6481 cttcctcgtt gggtccatca tcgggcgtgc gggacgtttc ttccttgtcg gggcaatgat
     6541 ctggagattc gggcccccga tcaaaacgtt catcgaccgg tatttcaacg cacttgccgt
     6601 agcttttacg gttttgctcg tgggcgggtt cgtcgccatc aagtacgcgt tctgagcact
     6661 tggatggggt tagtcgacag gggtgactat gagtggtttt cgcaagaaca gcgtcgcgta
     6721 ttcaccgaaa caatgggaga tgcggtggca tccgatccgg aaagagtggg tgggatttgc
     6781 cgcgcaccgc aatacacgac cctggcaggg ggaacaggcg ccgcagccgc ctccgccgcc
     6841 gcctttcgat ccgacgtgct acctctgccc cggcaacaca cggaaccggg gggaaagaaa
     6901 ccccgattat tcgggaacat tcgttttcga caacgatttt cctccttttg ggcccgatgc
     6961 gccggaaacc actaccgagc acggcctgta caaggcgatg ccggcgtacg gacgttgccg
     7021 ggttgtctgt tttcaccccc ggcacgatct cacccttgcc gaaatgaatc gggaagacat
     7081 tcgccgcgtg gtcgagacct ggcaggctga acacagaatg ctcgcaaagg atagccgtat
     7141 caaccacgtg ctgattttcg agaacaaagg gagcgtcgtg ggtgtgagca atccccaccc
     7201 gcacgggcag atttacgcaa cggagttcgt gcctccggtg accgtcacag agctcgcatc
     7261 atcccgggag ttctacgcag agaaacgccg cacgcttgtt ggagccattc tggagcagga
     7321 actgcgggat gaggttcgcg tcattgcgca gaacgatacg tcggttgctt tcatcccgta
     7381 ttttgcgcgg tacgcgcacg aaacatacat cgtaccgcgc cgccccatgc cgtcagtcgc
     7441 ggatctcacc ccggacgaag cggacgactt cacagagatc ttacatacga tgctcgtgaa
     7501 gttcgacaac ctgtacggta tttctttccc ctatgtgatg gcgttccatc aggctccaac
     7561 ggatggcgcc gcccacccgg aataccattt tcacgtcgaa ttccacccgc cactccgcta
     7621 cccgggagtg cttaagtacc tcgccggcgc ggaaaccgga gcgggggttt ttcttaacga
     7681 cacgtcaccc gaggagaaag cgaaggaatt ccgcgcggcc ggtaacatcc attacaaagc
     7741 aaccggcggc cagaagacag cctgatgttt ccgcacaaaa gaaaggcttg gaagtattta
     7801 ccccaagcct ttcgaacaat cgatcggcaa acgattactt gtctttgccg ggaaggttga
     7861 agaactcgcg catgttttcg tacaggactt tccggtcctc gggatcagcg agattgaggc
     7921 catattcgtt gatcaacatc acctctctgc gagtccactc gtcccacgct tcctgactca
     7981 cattctccca tatctcctga ccgattttcc cccgcatcgg cggtttttcc atctgcggca
     8041 cgaccttgcc ggttcgggag caggttatca tctttgccat gggaactcct tatcatgtat
     8101 ggcatggttt ggctactgaa cgaatccaaa gcatagcaac gcgcgccacg aatatcaatg
     8161 tgcgtcgaga gctttctttt acgaggcggc agttgtgaat accgaacatg agtgtctcat
     8221 ccggtgggag gaagttcaca agagctttgg agcgaaccac gtcctgcgcg gggtaacgct
     8281 ccaggtgtgc cagggcgaaa ccctggtcat catcgggcgt tcaggatgcg gcaaaagcgt
     8341 gttgctcaag acactggtgg gactcatctc cccggattcc ggcagaatct gggtgaaaga
     8401 tcgccagatt gtcggtgatc catccgcgat ggcggaagcc cagaggcttt gcgggatggt
     8461 gttccaggga gcggcgttgt tcgattctct ttcagtcgcc gaaaacctgg cattgcccta
     8521 ctgggagaac accgacctca gcaaggggga aatcgaggaa cggatacggt cattgctcgc
     8581 acttgtgggg ctgcgcgatg tagaacaact catgccggca gagttatcag gcggcatgcg
     8641 caaacgcgta agccttgcac gggcgctggc tgacgaaccc gagatcatgc tctacgacga
     8701 accaaccaca ggtctcgatc ctatcatggc agatgctatc aacgagctta tcaggtcgac
     8761 gcaggagaag gtcaaattga cgtcgatagt ggtaacgcat gacctgagta ccgtgcggaa
     8821 agttgcgaac catgttgcaa tgcttcatga ggggcgcatc gtattccacg ggactccgga
     8881 cgagttgttc gcgtcagccg atcccgtggt aacacagttc atccaggggc gcgcggacgc
     8941 gcgtgtcgag gtcccgcgcc cggaactgcg cgagcacgaa atcgatgagt aagccgaacc
     9001 gggaagagcg ccgatcggcc tcgtcgagtt gggtttcctg gctcgtggaa atggcgcgtg
     9061 cggagaggct ctggtttggc gggaccgtgt tgttttccgt tatgggcgct ctcacgggag
     9121 cgctcgccac gtggctgatg gttcccctgg cggctgttct gttccccgaa agcactggag
     9181 cggacgcgat tcacaacccc tcaagagggc tgtatcccgt ttctgcaggt gcctggttga
     9241 cgtcggcaac cgcaagcatc acacccctct cagtcgctct ctcattcgtt gttgtcttca
     9301 ccgtgaagaa tctctgcgag ttcggctcgc gcgtggggtg cgacgccctg gtcgcaaagg
     9361 ttgaattccg gctgcgcaat tccctgtggc gccagtatct ggcatgtggg cgcgcgtcag
     9421 ggcgttcttt ttccggggcg cgattcatcc acgaactgct tgccggcgcg ggggagccgg
     9481 ccgccgcggt cggagcaatt ccggtgcgtt ggatcggtga cccggtcgct gcggtggccc
     9541 ttctcggcac gatggcgata atcagccccc atcttacgtc cgcgatgttg ctgcttcttc
     9601 cacccgcgtg gtgggcaacc cggaagggct tccgccacat tggcaaccga acggagacaa
     9661 ggcttgccgc gcggggacga cttggagcgc gtttgagtga actcgcctcg ttccgcccgg
     9721 tgctggcaag ccacgccgcg atggattggg cactgatcaa caccggccgg cacgagcagt
     9781 cggtaatccg ggcagccctt tcatggtccc gcgttgctcg tttgatcccg ctgactgccg
     9841 agaccctcgg cgcgttcgtc ggcgccgccg taattctcct cgttgcaaaa ggcatacgtg
     9901 cgggaacgct ttcgggcgga gagttcttga ccttcctcac tgcgtttttc cttctccttc
     9961 cagtggcaaa gcggctggca gcgttaagtg gagacatgcg aacatcccgt tctgcgtggg
    10021 aaaggctcgc ttccttgctt cctgctgacg caagagccga agtggctgta cccccttcgg
    10081 aaacgttccg tgcacccgcg attactctcc gcaaacttcg ggcaagcatt cccggcggca
    10141 accgggtgct cgacatcggg tacgcagaat tcccccccgg agaggtgact gccatcgttg
    10201 gtccgaccgg ttccgggaag agcctgcttt tggaaatcat cgcggggttt ggtgttccag
    10261 acgaaggaac ggtatgctgg aacgaacggc cgatctcctc gccacatgtg gttccagaaa
    10321 ggccgccgtt cgggtacgtt ccccaggatc ggtggacaat ctcaggcaca gtcggcgaaa
    10381 acgtgacgct cgggagaccg gtttcggaaa ccgccctgac aacgactctc caggctctgg
    10441 ggttggatct ggacgttcag accgaactgg ggcaggacgg cgcgccgctt tccggtggcg
    10501 agaggcagcg cctggcgctg gcaagagcgt tgctcggtga gcctccgctg ctgcttctcg
    10561 acgaacccac ctcagcgctc gacgcggcga gcgaggaacg cgtcatcgct cttctacgct
    10621 ccctgaaggg cagaaccacg gttctggtgg caacgcacag cactgccgtc atcagtgccg
    10681 ccgactgggt gtttcatttg cctttccaat ctgcgacaac cgctctgcac aaggaaacgg
    10741 tgagataatg cgggtcaaaa tgctcgcggc cggatgcgtg ttcgcccttt cgtggtgcac
    10801 ttcgccccgt gcggatgtga cctctcgcta cgctgcgcaa tttgaggaag ccctcgccag
    10861 catggcgatg tcgcccgcag acgtggcatt ccgaacagat cacgtcgaac gggattcctt
    10921 ccggctccga cgaacggacg tattccttct tcaccccctc tccttcgccg actcgcttgc
    10981 atcgttctcc gcaagagcgg gcgagttcgg aggtccctcg ccgtatgtcc acctcgcggc
    11041 ggattggctt gatgatcccc tccccccgtt gtgcgagcct gctgcgcgag tgcttgtcag
    11101 agatacaaca cgatgcgcac gtgcgttcaa tcgcctggca ggtgcaatcg aacatgcggc
    11161 gcgcattgtt caagagagcc agagcgtttt ctctccggcg gaacgggagt cgctctcaac
    11221 atgggtagac agagtgctgt cggaggggga cccggacgta tcaggagaac aagtagacct
    11281 gttcgcgtgg aaagctgaag agctggcgga attgcagtgc tcgcgggcta atcttgaact
    11341 cgcaaaaaga tggagtggac gggaaattct ccgcgccgcg agttgcgtcg cgtcggcagc
    11401 ctgggagttg cgtgaagcgc tcatggaagc gaagaaccgg gtgcccatgc cgttgcctgt
    11461 taaaacatgg cacaccacct gcggacgtat gaccgtggga agcgacgccc aagacaacta
    11521 cacaggagat ttcgcgctca tcgttgatca gagcggagac gacacgtacc gcgtgcggcc
    11581 tcccggttct ggcggcgggc aggtgcaggt tgtcatcgac atttccggca acgacgtcta
    11641 cttcggaagc atcgcgggaa gcacgttcgg aatcgcgatc tccctcgacg tctcaggcaa
    11701 tgacacctac ataggcgagg cctggacgca aggctccgcc cgttttggcg cggcgatcct
    11761 gtgggatgaa cgaggaaacg atacctatta cgccaaccga gcggtgcagg gaacgggtat
    11821 gttcggactc gggctgcttg tggacgtgga cgggaatgac ctctatcgat cgggcaccta
    11881 cggccagggc ttcggggcca ccggcggaat cggcattctg gacgatcgaa agggaaacga
    11941 ttcgtactac gcgggaggcg cgactactga cgtgttgcgc tttgatgacc actacctcac
    12001 catggcacag ggggtcggat cgggcattcg tcccgttggg tccggcgggt tcggttttct
    12061 gattgatcgg aaggggaatg acgtctacaa tgccgacgtg tacggacaag gcgtgggtta
    12121 ctggctgggc ggtggcgggc ttcttgacgg tgaagggcat gaccgctacg tggcgcatca
    12181 gtacgcacag ggtgccggaa tccacctttc ctcgggcgct cttctcgatt tccgcggaga
    12241 tgatgtctat gtgatcaacg gcgttggcca gggatgcggg cacgacctca gcgtgggaat
    12301 tctcttcgac gaaactggcg acgacgcatt caccgtggaa ggcctcgctc tgggtgccgg
    12361 gaacgcaaac gggatcagcg tgttcgcgaa cgtgagcggt cgtgacgcgt acatcgcccg
    12421 gcgtgaggac gtcatgggat acagtgatag acggcgcgaa tatggtatga tcggagtcat
    12481 gctcgacctc gggggcgaag accgctatgc agcacccttg ggcgccaatg acaggtggtg
    12541 gcaccattca acgttcgggg ccggagtcga tctgggctcc gtttctccct cttccgatac
    12601 cgcgaccgcg gctgatctgt cactcaccga gggaggacac cgccagaaga ttgcggcgac
    12661 tgctgagtct ttgtttgtcc aggcttcgaa cccgttttcc gcgctccagt accttgttga
    12721 accagcagaa aaccgacttg ccgacatggt gggggaatct gtgacggcgt tctgggcagg
    12781 caaactcgcc tcagaaagcg cgagggaacg ctgggccctc gtgcgtatcc atcagaagtt
    12841 gtttgcccga ggggacctca gcagcgtccc gatgttgatc gattcgctcg aatccccttc
    12901 gggcgcggca cgtcgtatgg ccgcacatct cctaggcttt tcg
//
LOCUS       DAOTNG010000051        12808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4714_length_12808_cov_5.714891, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000051 DAOTNG010000000
VERSION     DAOTNG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_12808_cov_5.714891"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="P5179_03875"
     CDS             complement(<1..322)
                     /locus_tag="P5179_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94389.1"
                     /translation="MKGMQYWGGGKPVIPLAALGQGDYVKEWLIIGPFDLSSSFHLRR
                     EQEIGRADAIDLDPIGGERFVKPVEGAAHANAHLPAGCATWRRCSGTPVVDLRRQFPE
                     FPTAL"
     gene            499..1140
                     /locus_tag="P5179_03880"
     CDS             499..1140
                     /locus_tag="P5179_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRS94390.1"
                     /translation="MIPMLPPPNDAVVLFNGKDLSNWKTRDGKPVGWQIANGEIHVIR
                     GTGDIVSDKRFTDFYLHLEFNCPDMPEAKGQAKGNSGVYLQGRYEIQVLDSFGWNVPG
                     KGDCGAIYDQFSPLVNACKPALHWQTYDAVFRAPRFDGNGNRVSGARVTLFHNGRVVH
                     NNVELPGPTGGALDQNVAEPGPLLLQDHGDAVRYRNIWIVPLPLTGSDQYAPA"
     gene            complement(1201..2934)
                     /locus_tag="P5179_03885"
     CDS             complement(1201..2934)
                     /locus_tag="P5179_03885"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRS94391.1"
                     /translation="METLRLGLAQINTTVGDISRNTAKVLEWVRRAREEGVDAVIFPE
                     LTTVGYPPEDLLFRTEFLRENLRARDEIVAASEGIAVVFGFVDCPDDIFNAAAIACDG
                     ALCGVYHKRFLPNYGVFDENRYFQAGVAQRVFSIGEFRLGISICEDIWYPGGPADEQA
                     LIGGAHILINVSASPYYEGKIGSRDRMVSVRAADNDAVVALVNLVGGQDELVFDGGSI
                     ICSENGHVRALGRQFEEDLICTDVHLDTILRSRLRDPRRRKEKLAALLRREEIPCTPL
                     PVRLRERPVAVPSAPASRLGRTEEVYRALVLGVRDYVGKNGFPGVVVALSGGIDSALT
                     AAIAVDALGKERVAGVTMPSRFNSDETQNDAATVAANLGIRFIQLPIEPVVSAMLDTL
                     RDVFRGLEPDTTEENLQARIRGTLVMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAG
                     GFAAIKDVYKTMVYELCEWRNAQAGYDLIPRTTIARPPSAELRPNQLDQDSLPPYDVL
                     DRILEAYVEMDESLAEITARGFDQAVVSDVMRRVDSSEYKRRQAPPGVKITPRALGKD
                     RRLPITAPRSR"
     gene            complement(2965..3591)
                     /locus_tag="P5179_03890"
     CDS             complement(2965..3591)
                     /locus_tag="P5179_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94392.1"
                     /translation="MSTRPFCRIVRPASSFLRNGARATFAVILSVFACSRAGAVTVSA
                     GAGFGVPTAMGGYLKTSRAFEVTTFARLTETASLGLLADYTQQPTRTAVTTSYQERLL
                     VIVRWTWGERQFPNWFQPFGQLGVGGGYMEYAGPSLVEHLNAPAGRLGIGANVHINKR
                     LAIVPTLSYDRLEAASIQAERRYPTMLTLRCSAAFSVFGDNPLLVPAR"
     gene            3796..11238
                     /locus_tag="P5179_03895"
     CDS             3796..11238
                     /locus_tag="P5179_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94393.1"
                     /translation="MATTRERSAKKKEAPSQTHERVFFPVRCAAELDGPQAGNRSRFA
                     GRAKPAASEIAALLCGKLWGRAAKDIVLVKDAFSVCAVRQSTPPRVEIAGDVLEALGL
                     RLSGKQITGPAETVSGAIAVLRLAKAWSDEFRRTSDARAALRAALDAWADFPATDREA
                     CLDVLAMPPLDTGKVFSAFLRLAGDEKTRSQQGESADAWRERWITWVLGQMRIDLPYD
                     RTAVRDILSSVDDPDESRAMLYQAIRKYDRGIEQDNAARLAEELAWAREELIFGRMSR
                     AFHNQALLFASSRYITVTAEMVRAGARLAAAASVWPALRPAVDRVTLLLQPHMEVPHT
                     ELAGAIDHVEDVAVQQQKTYVQKVLEQERGQSSQTGATEGRTGALEAAYSSGKRILAS
                     TRAIRASLAKSPRRPAAYVVISQRPSPTGSHLVARINEGQDPFLGKPEDLRELVRYAG
                     DRLYASPDYAWLEYANHWIEAIPLFIKERIRVDNGVEIAETVIDQEGMEESFREQMAD
                     YWERNIARTELSGMAALARALMSGEDPEGSDLSLSADLSRARKALRNGAGGQIARLTA
                     ALAIGAETNAFEISMAAESSGDRLEATRRVLFGGDAKPGLWTSVLEIRKHFADWPKAV
                     DEALSKASENDKNRFNSLIPEAERLATIPRRPLPVLHVLTTQSARMTDGYIRTWLEES
                     MALSRIAREYGLDADVRQRMDDYAREIEAIAESLVKELGLEPEIESIMATERVNRRAA
                     ILRLVGANRIFQKQVSLIAVLREVRGVAKGDLNAPLDSPELDAFTAEHEREISDKALA
                     KVVERNVDEFARSIAEMGASASREDLHSLQRKVVAENSDLRADLDTFTRFESREGFFE
                     DLSARHPDWKLKERRETWLRKHQRLALTTARKEIIAEQGLNHLTLDPRFFLEAVGGNK
                     RYHLIYTPSRVDLGPKERESVETWSQWVGGRDKEAAQAGRRIYGLINKSVKRFNSLTE
                     PEILKTGENASMAAHFAYSNAMALMVNAAGRGDMEQLGDEMSLRNDRIIHPAGEGYGG
                     YCVPKDGLFLEFVLTLSERVKLRQLGIEERFHEAVSQLARKILLKREDFPCDLEWERW
                     AEELLGREKGLQGLFSTHSGEGPGAANHRIPVFQITRLATVLEQLGQPPLHDNRAVVK
                     SLAARWGIHTMVAGAEHVNRFMPFYKAWLIYDTIAEARRFDPSVASPEESVIVLSAEY
                     KPDTQDGRFSVGMRKYEILAGTGDHLRYSLGYEAATLASLMFDGWSVVESRRGVDDPV
                     LEQIASAWVGSKDDVRSALKFVFPGYGPPAEMRLVSPMGLSKHDVLRYTSDTRLEEYA
                     EEARVALVTAGLSDEDIRAHMATFGPRLERWTRLRNRGAAELAGCLKGKVHALALAVL
                     GPERSYEHALQGADVFDVGIPHRQVMELLNEPARLCDLMLFGKPGSALAIVDGASGAR
                     RRALNRRGVMLWFAAAENRGKRGVYRSIGLGEDTVEEWRSQMREQRQRALEYADALRA
                     GRKAVDSAFERVITQLRSEQEASLVLEEEERLRRFSRDTERDHVVAMLLAEIVTMRSP
                     RELTFAHWLALGGMFVLEGCSPAAFEEGYVSFEKARGKLQPGSEEPRTAAEIKRLAGL
                     ISRPLVRAYGSGFRQVGGVESSNKATEEVKHIALSTRRQLADRARRLMSARVRADSFQ
                     TTWNEPRLRHKSLEELNADVREALGEPEVFASEALLGKLVALARVFGEKLLAEICPLA
                     SPDREKASDAIAALLNEREFDVSALKGVCGSYEDIGTIGRMAQELSERAARENWTAEA
                     SDAAFEKLADFAEFVSTIHALEISLADRQRNPDEVDAPSLWRQLASYFAETLNDHFYE
                     YRPWAYSRGVGFQRYTGDRLYALANRHFAWLYRYLRHLIVTRTEVRYLTPVQQDLLIG
                     RISEAGVVVAVGASGDTEEEKRWRAFNQLREIAFIRNDGFPLPPTFDGFDPALIDADN
                     RANILSMHPVGRTHVSRLVAEGPTLARELVVAGQPAANVILTRSVRVDRDSVLVDDGH
                     LYVDKQTYVQALRDNWGLSETGAKALAERDVGPKGVRIAVRFSRPVRAAVVLPMHGNP
                     VYDGGHLERLGLPYSVQSRFHTWTTYDKAKYPDIFTPETGVRIPAEIDWLHEWTVAGE
                     EEEIKHQIRSGRPGTDYCGLQRFSEKYGIVMVKDAAESGGRGQKPFPLRTAVGSLNEE
                     TLSEAVDFLYQISLVHNVSVQEVVLSSPETWATEEFLERFVDRQVTEWYRPITRDRQP
                     ATPLFGSLRVIVSTDRPLAEDRYHHWHMSHRISLNSTQLITNVGRGGTLDLLRPEDIR
                     PEFRDTILAALDDAARRTMEAMAAYEAKAGARYTLETGLPVGKDASGVSYGIPRYLML
                     DFLLRPVFHRKGDVVETVPRIDEKGNRIGSVFMLRDGNDVFEGEIVDWEVILIEPNIG
                     IGLWDRVAIREEELERKRAQATGRPMDWDRVGENARVVLRDLTRAGIDYLEAKRRGST
                     "
     gene            11284..12678
                     /locus_tag="P5179_03900"
     CDS             11284..12678
                     /locus_tag="P5179_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRS94394.1"
                     /translation="METRFEPTQYLVQAAADALLEYENGPDLTFAAGDWEAVFGAAYR
                     WFRAFGRESLEDLSQNAPDSVTAGEKLLNECPDRLAMLVADALTTNSIAPPVCISLTP
                     VHLDTPYGAVVFAAFPRAIEELAPLLVDGLEEQMRSRRFSGVVAIHDTWMQKDGYPER
                     ILRLDAENNVIVEQGVLNPCRPERILLLLGPGSDEATWHRRWHDVASNCGIDVANPYE
                     FAYIADDKWQTFHILQNAGVPTPVTRLVPPDTAFTAEMCLSLVDGPSAPVVAKPRHGT
                     EGLAATVCNPAETTDPLKAIWERGDDALLQPFTNDLVWVDEVGEVHTAVLRMNVTCNA
                     QGASRAESGFVHVAAHGCGIASVGHGGKLRPILRSALRVRIGGRLAPFPVHLTTQCQA
                     CACAAVDAVAAASRKTRLAGVDIVVSAGDDGVPRALALEVNPRPAGLSHSRFVSAGWE
                     TEEEPGVSRLMWGE"
BASE COUNT         2702 a         3626 c         3971 g         2509 t
ORIGIN      
        1 caagggcggt gggaaattcc ggaaattggc gtctgaggtc gaccacggga gtgccgctgc
       61 acctgcgcca cgtggcgcac ccggccggaa ggtgcgcgtt cgcgtgagcg gccccttcca
      121 caggtttcac gaatctctct cctccaatgg gatcgagatc tattgcgtcg gcgcggccga
      181 tttcctgttc acgccgaaga tggaagctgg aggagaggtc aaacggcccg attatcagcc
      241 attctttcac gtaatctccc tgaccgaggg cggcgagagg gatcaccggt ttcccgcctc
      301 cccagtattg catgcctttc attcgttgct ccctgtttgt ccgcagaagg tgacgatgct
      361 tttcatttgg cgactctccc gcaccgaagg gtggctacgg gcgacaaacg tttggttctg
      421 tagaaatcgg ttcgtacgtt ttcagcgttg cgtcaggtac gagcagggct ggaaccgttg
      481 ctcttgaaag gagaaaccat gattcccatg cttcccccac ccaacgacgc cgttgtgctt
      541 ttcaacggca aagatctctc caactggaag accagggacg gaaaacccgt cggctggcag
      601 atcgccaacg gcgaaatcca cgtcattcgc ggaaccggtg acattgtgtc cgacaaacgg
      661 ttcacggatt tctacctgca tctcgaattc aactgccctg acatgccgga ggcgaaaggg
      721 caggcaaaag gcaacagcgg cgtatatctc caaggacgat acgagataca ggtgctggat
      781 tctttcgggt ggaatgtgcc cggaaaaggt gattgcggcg caatctatga tcagttcagt
      841 cctctggtga acgcctgcaa accggcactg cactggcaga cgtacgatgc ggtcttccgc
      901 gcaccgcggt ttgatggcaa cggaaaccgt gtaagcggcg cccgggtcac tcttttccac
      961 aacggccgag tggtgcacaa caacgtcgaa ttgccgggcc cgaccggagg ggctctggac
     1021 cagaatgtgg cagaacctgg tccccttctc ctgcaggacc acggggacgc agtgcgctac
     1081 cggaatatct ggatcgttcc gcttcctctg acaggctccg accagtacgc cccggcgtag
     1141 gaaagcctcg ggttctttcg ggcgcctcgt cggtccaact tgaccggcga ggcgcttcgt
     1201 ttatcgggat cgcggtgcgg tgatcggaag tcggcgatcc tttccgagtg cccgcggcgt
     1261 gattttgact cctgggggcg cctggcgacg cttgtattcg ctggaatcaa ctctgcgcat
     1321 tacgtcgctt acgaccgctt gatcgaaacc acgcgctgtg atttccgcga gagactcatc
     1381 catctccaca tacgcctcga gtatgcggtc gagcacgtcg tacgggggaa ggctatcctg
     1441 atcgagctga ttcggccgga gctctgctga gggtggacga gcgatggttg tgcgggggat
     1501 caggtcataa ccagcctggg cgtttctcca ttcgcaaagc tcgtatacca tcgttttgta
     1561 gacgtctttg attgccgcga acccgcccgc catgtcaccg tacagcgtgg cgtacccaac
     1621 tgacatttcg ctcttgttcc ccgtagtcag tacaagccac ccgaacttgt ttgaaagcgc
     1681 catcaccagc gttccgcgta tgcgggcttg caggttctct tcggtggtgt ccggctccag
     1741 gccacggaag acgtctcgca aagtgtcaag catggcggac accacaggtt cgataggaag
     1801 ctggatgaag cgaattccga ggtttgccgc gacagtggct gcgtcattct gtgtctcgtc
     1861 tgagttgaac cgtgacggca tcgtgactcc agccacgcgt tctttcccca gtgcatccac
     1921 cgcgatggcc gccgtaagag ctgagtctat tcctccggac aatgctacga cgacgccggg
     1981 gaacccgttt ttccctacgt agtctcgcac cccgagcact agtgcgcggt acacctcttc
     2041 cgtcctgccg agtcgcgagg caggcgcgga tggaacggcg acaggacgtt cgcggagccg
     2101 cacgggcaga ggcgtacacg ggatttcctc tctccgcaga agcgcggcca gtttctcctt
     2161 gcgccgccgc gggtcacgca ggcgcgaccg cagaatcgta tcgaggtgta catccgtgca
     2221 gatgagatcc tcctcaaatt gcctcccgag ggcgcgcaca tgcccatttt ccgagcagat
     2281 tatgctccct ccgtcgaaaa ccagttcatc ctggccaccg accaggttga ccagagccac
     2341 caccgcgtcg ttgtccgctg ctctgacgga taccatgcgg tcgcggctgc cgatcttccc
     2401 ttcgtaatac ggtgaggctg agacgttgat gaggatgtgt gcgccgccga tcagcgcctg
     2461 ttcatcggca ggtccgccgg ggtaccagat gtcctcacag atgctgatgc cgagcctgaa
     2521 ttccccgatg gagaaaaccc gttgcgcgac tcccgcctgg aaatacctgt tttcatcgaa
     2581 tacgccgtag ttcgggagaa atcgtttgtg gtacacgccg caaagggcac catcgcatgc
     2641 aatggcggcg gcgttgaaaa tatcatccgg gcagtctacg aagccgaaca caacggctat
     2701 accctcactt gcggcaacga tttcatcgcg tgcccgcaga ttctcgcgca gaaactccgt
     2761 ccggaagagg aggtcttccg gcgggtatcc cacagtagtc agttcgggga aaatcaccgc
     2821 gtcgacgcct tcctcccgcg cacggcggac ccattcaagc actttggcgg tgttcctcga
     2881 gatgtcgccg acggtggtgt tgatttgggc aagtccaaga cgaagagttt ccatcagtcc
     2941 attactccgt gtcgtggccc cggatcatct cgccggaaca agaagcgggt tgtcgccaaa
     3001 aaccgaaaac gcggcggagc agcgcagtgt aagcattgtg gggtatcgac gttcggcctg
     3061 gatcgaagcg gcttcaaggc gatcgtacga caacgtggga acgatggcga gccgtttgtt
     3121 gatgtgcaca ttggcaccta tgccaaggcg gcccgcaggt gcgttcagat gctcaaccag
     3181 tgatggccct gcgtactcca tgtatccccc gcctacgccc aactgaccga atggttggaa
     3241 ccagttcggg aactggcgct caccccaggt ccagcgcaca attaccagca ggcgctcctg
     3301 ataactggtt gtaaccgcag ttcgagtcgg ctgttgcgtg tagtccgcca gcaggccaag
     3361 agaagccgtt tccgtgagcc gtgcgaacgt ggtcacctca aatgcgcgcg aggtcttgag
     3421 gtaccctccc atcgcggtgg gaaccccgaa gccggccccg gcggacacag ttactgcccc
     3481 ggcccgactg cacgcgaaca ccgacaggat gaccgcgaag gttgctcgtg cgccgttccg
     3541 caggaaactg ctggcagggc gtacaatgcg acagaatggc cgagtggaca tacatggaaa
     3601 ctacaccccg cacattcggg aaacaagcgg cgcacgggcg ttgcagcaga ggccgctcgc
     3661 tccgcgacga gtacgcaccc atgagcggtt atcgttgccc cgtcagagcc cgcaggggta
     3721 tcttttccga aagcaaaatt ccagtcgtat ctctcgaatt gggtgcgacc ggcctgactt
     3781 catccagggg tactcatggc aacaacacga gagcgttccg cgaaaaagaa agaagcaccg
     3841 tcgcagactc acgaacgagt ttttttcccc gttcgttgtg ccgccgaact ggacggcccg
     3901 caggcgggca acaggagccg atttgccgga agggcgaagc ctgcagcttc cgaaatcgcg
     3961 gcgttgctct gcggcaagct gtggggccgt gcggcgaaag acatcgtctt ggtcaaggat
     4021 gcgttcagcg tgtgcgcagt caggcagagc actccaccca gggttgagat cgcgggagat
     4081 gtcctggaag cactcggcct gcgactgagc gggaagcaga tcaccgggcc cgcagagact
     4141 gtctctgggg cgattgccgt ccttcgcctc gccaaagcct ggtccgacga gttccgacgc
     4201 acttcagacg cgcgtgcggc cctgcgcgct gcactcgatg cctgggcgga tttcccggcg
     4261 accgacaggg aagcgtgtct ggacgtgctg gctatgcctc ctctggacac cgggaaagtg
     4321 ttctccgcgt tcctgcgact ggcgggcgat gaaaagacgc gatcccagca gggcgagtcg
     4381 gcggatgcct ggcgcgaacg ctggattaca tgggtgctcg gacagatgcg gatcgatctg
     4441 ccgtacgaca ggactgcggt tcgcgacatt ctgtcatcgg tggatgaccc cgatgaatcg
     4501 cgggcgatgc tctaccaggc tatcaggaaa tacgaccgag gaatcgaaca ggacaacgcg
     4561 gcgcggttgg cggaggagct ggcgtgggcg cgggaagaac tgatcttcgg gcgaatgagc
     4621 cgtgcgttcc ataatcaggc actgcttttt gcgagttccc gttacatcac agtgactgcc
     4681 gagatggtga gagcgggcgc gagactcgcc gccgcggcct ctgtatggcc ggccctccgc
     4741 ccggcggtag atcgagtgac tttgctcctg caacctcaca tggaggtgcc tcacaccgaa
     4801 ctcgccggtg cgatcgatca cgtggaggac gttgccgtcc agcagcagaa aacgtacgtc
     4861 cagaaggtcc tcgaacagga acgcggtcag agctcccaga ccggtgcgac agagggccga
     4921 acaggcgctc tggaagccgc atattcctca ggcaagcgca ttctcgcgtc aacacgggca
     4981 attcgagctt cgctggcgaa atctcctcga agacccgccg cgtacgtagt catcagccag
     5041 cgcccgtcac caacagggtc ccacctcgtg gcacgtatta acgagggcca ggatccgttt
     5101 cttggaaaac ctgaggacct gcgcgaactg gtccgttacg ctggtgacag gttgtatgcg
     5161 tctccggact acgcgtggct tgagtacgcg aatcactgga tagaagcaat tccgctgttc
     5221 atcaaggagc gaatccgagt cgataacggg gtcgagatcg cggaaacagt cattgatcag
     5281 gaggggatgg aagagagttt ccgtgaacag atggcggact actgggaacg gaacattgcg
     5341 cgaaccgaat tgagtggcat ggcagcactc gcgagggcac tgatgtccgg tgaggatccg
     5401 gaaggaagtg acctttcgct ctccgctgat ctctcccgtg cgcggaaggc gcttcgcaac
     5461 ggagcaggag gacaaatagc acgattgaca gccgcgctgg caatcggtgc agaaacaaac
     5521 gccttcgaaa tcagcatggc tgcagagtcg tccggcgaca ggctggaagc cacccgaagg
     5581 gtgttattcg gaggggacgc caaacccggc ctgtggacct cagttctgga gattcgaaag
     5641 catttcgcgg attggcccaa ggcagtggac gaggcgttgt cgaaggccag cgagaacgac
     5701 aagaatcgtt tcaactcgct tattcccgaa gcagaacgtc tcgcaaccat tcctcgacga
     5761 cctttgccgg tcctccacgt ccttacgaca caatccgcac gcatgacgga cgggtacata
     5821 cgaacctggc tcgaagaaag catggcgctc tcccgcattg ctcgtgagta cggccttgat
     5881 gcggacgtca ggcagcgcat ggatgactat gcgcgcgaga ttgaagcaat cgccgagtct
     5941 ctggtgaaag aactggggct ggaacccgaa atagaatcca tcatggcgac agagcgtgtg
     6001 aacaggcgcg ccgccattct tcggcttgta ggcgccaacc gcattttcca gaaacaggtc
     6061 agtttgatcg cggtacttag agaggttcgc ggcgtagcca agggggattt gaacgctcct
     6121 ctcgattcac ctgaacttga cgctttcacc gcagaacacg aacgggagat ctccgacaaa
     6181 gccctcgcca aggtagtcga aagaaatgtc gatgagttcg cacgttccat cgccgaaatg
     6241 ggtgccagcg cttctaggga ggacctgcat tccctccaac gcaaagtagt cgccgaaaac
     6301 agcgatctcc gcgcggatct cgatacgttc acgcgtttcg aaagcagaga ggggtttttc
     6361 gaggacctgt cggcacgcca tcctgactgg aagctcaaag aacgacgcga aacgtggctg
     6421 agaaagcatc aaaggcttgc gctgacaact gctcggaagg agatcatcgc ggaacagggc
     6481 ctgaaccatc tcacgctcga tccacgtttc ttcctggaag cagtgggcgg aaacaagcgt
     6541 taccacctca tttacacacc aagccgagtg gaccttggtc ccaaggaacg ggagtcagtc
     6601 gaaacctggt cgcaatgggt aggcgggcgc gataaggagg ctgcgcaggc cgggagaagg
     6661 atatacggcc tgatcaacaa gtcggtgaag cggttcaaca gcctcacgga accagaaatc
     6721 ctcaaaaccg gtgagaatgc ctccatggcg gcgcatttcg cctactccaa cgccatggcg
     6781 ctcatggtca atgccgccgg ccgtggggat atggagcaat tgggcgacga gatgagcctc
     6841 cgcaacgacc gcatcataca tcctgccggg gaggggtacg gtggctattg cgtgccgaag
     6901 gatgggttgt ttctggagtt tgttctcacg ctttctgaac gcgtcaaact ccgccagctt
     6961 ggcatcgaag aacggtttca cgaagccgtt tcacaacttg cgcgtaaaat ccttctgaaa
     7021 cgggaagact tcccgtgtga cctggaatgg gaacgctggg cggaggagct tctcggacga
     7081 gagaaagggc tccaggggct tttttcgaca cattcggggg aaggcccggg cgctgcgaat
     7141 caccgtatcc ccgtgttcca gatcactcgt ctcgccactg tgctcgagca actggggcag
     7201 ccaccattgc atgacaaccg cgcggtggtg aagagcctcg cggcccgatg gggtatccac
     7261 accatggtcg cgggtgcgga acacgtgaat cggttcatgc ctttttacaa agcgtggctc
     7321 atatacgaca ccattgccga ggcccgccgt ttcgacccgt ctgtggcatc tcctgaggaa
     7381 tcagtgattg tgctttccgc ggagtacaag cccgacacgc aggatgggcg attctctgtg
     7441 ggcatgcgga agtacgaaat cctcgccgga acaggtgacc acctccgcta cagcctcgga
     7501 tacgaggccg ccaccctcgc atcgctgatg ttcgatgggt ggtccgtcgt ggaatcccga
     7561 cgtggcgtgg atgatcccgt tcttgaacag attgccagtg catgggttgg cagcaaggat
     7621 gatgtgcgtt cggccctgaa attcgtgttc cctggctacg gacctccggc ggaaatgcgc
     7681 ctggttagtc ccatggggct ttcgaagcat gatgtgctgc ggtacacgag cgacactcga
     7741 ctggaggagt atgcggagga agcgagggtc gcgctggtaa ctgcggggct gtccgatgaa
     7801 gacatccgag ctcatatggc taccttcggc ccccgactgg aacgatggac caggctgcgc
     7861 aatcgcggcg cggccgaact tgccgggtgc ctgaaaggga aagtgcacgc gctcgcattg
     7921 gcagtattgg ggccggagcg ttcatatgaa cacgcgctgc agggtgcaga cgtgttcgat
     7981 gtcggaattc cgcacaggca ggttatggag ttgctgaacg aaccggcgcg tctgtgcgac
     8041 ctgatgctgt tcgggaaacc gggaagcgcc cttgcgatcg tagatggggc gtctggcgcg
     8101 cgaaggaggg cgcttaatcg ccggggtgtg atgctctggt ttgccgctgc ggaaaaccgc
     8161 gggaaaagag gcgtgtatcg ctcgataggg cttggtgaag acaccgtcga ggaatggcgg
     8221 tcccagatgc gtgaacagcg tcagcgggcg ttggaatacg cggacgcgtt gcgtgcaggc
     8281 cggaaggcgg tggactcggc attcgaacgt gtcatcacgc agcttcgcag tgagcaggag
     8341 gcttcgctcg tgttggaaga ggaggagcgg cttcgcaggt tttcacgaga taccgagcgc
     8401 gatcatgtcg tggcgatgct gctcgccgaa atcgtaacga tgcggagccc aagggaactg
     8461 acattcgcgc actggcttgc actgggtggc atgtttgtcc tcgaggggtg ttctcctgcc
     8521 gcctttgagg agggatatgt ttcgttcgaa aaagcgcgtg gcaaacttca acccggttcc
     8581 gaagaaccca gaaccgccgc ggagatcaag cgacttgccg gcctcataag tcgtcctctg
     8641 gtgcgtgcgt acgggagcgg attccgtcag gtgggcggag tggagagctc gaacaaagcc
     8701 acggaagagg tgaaacacat tgcgttgagc acaaggcgcc agcttgccga ccgcgcacgc
     8761 agattgatga gcgcacgagt gcgcgcggat tccttccaga ccacgtggaa tgaacctcgg
     8821 cttcgacaca aaagcctgga agaactcaac gccgacgtgc gcgaggctct tggagaaccg
     8881 gaagtcttcg cctcagaagc ccttctggga aaacttgtcg ctcttgcgcg cgtcttcggg
     8941 gagaagcttc ttgctgagat ctgcccgctg gcatcgccgg atcgggaaaa ggcaagcgac
     9001 gccattgccg cgctcttgaa cgagcgcgag ttcgacgttt cagcactgaa aggtgtctgc
     9061 ggaagttatg aggacattgg tacaatcggc agaatggcac aggagttgtc cgaacgtgcc
     9121 gcaagggaga actggactgc ggaggcaagt gatgcggcgt tcgaaaaact ggctgatttc
     9181 gcggagtttg tgagcacgat tcacgccctc gaaatatcac tggccgaccg acagcgcaat
     9241 cctgacgagg tggatgcgcc aagcctttgg cggcaactcg cgtcttattt cgccgagacg
     9301 ctgaacgacc atttctacga gtaccgtccg tgggcatact cacgcggggt gggcttccag
     9361 aggtacacgg gagaccggct gtatgccctc gccaaccgcc atttcgcatg gttgtatcgg
     9421 tacctccgcc acctgattgt cactcggacg gaagtgaggt atctgacgcc tgtccaacag
     9481 gatcttctga tcgggaggat cagcgaagcc ggagttgttg tggcagtcgg tgcatcgggt
     9541 gacaccgaag aggagaaacg ttggagagca ttcaatcaac ttcgtgaaat agcgttcatt
     9601 cggaacgacg ggttccccct ccctccgacg ttcgacggct tcgatcccgc tttgattgat
     9661 gcagacaaca gagcgaatat cctgtccatg catccggtcg gacgcaccca cgtatcacgt
     9721 ctcgtcgcgg aagggcccac tcttgcgcgt gaactggttg ttgccggtca gccggcggca
     9781 aatgtgattc tgacacggtc agtgcgtgtt gaccgtgatt cggttctggt tgacgatggc
     9841 cacctttacg tggacaagca gacctacgta caggcgctgc gagataactg gggcctcagc
     9901 gaaaccggtg caaaggcgct cgccgaacga gatgtcggac caaaaggtgt ccggatcgcg
     9961 gttcggtttt cgcgacccgt gagggcagcc gtcgttctgc cgatgcatgg gaatcccgtg
    10021 tacgacgggg gacacctcga acggttgggg cttccgtaca gcgttcagtc gcggttccat
    10081 acctggacaa cgtacgacaa ggccaaatac cccgacattt tcaccccgga aacgggcgta
    10141 agaattccgg ccgaaattga ctggcttcat gaatggacgg tcgctgggga agaggaggag
    10201 atcaagcatc agatccgttc cgggcgaccg ggaaccgatt actgtgggct tcagcggttt
    10261 tcagagaagt acggcatcgt gatggtcaaa gacgctgcag aatcgggcgg aagagggcag
    10321 aaaccgtttc cgttgcgcac ggctgtcgga tcattgaacg aagaaacact gagcgaagca
    10381 gtagacttcc tctatcagat ctctctcgtt cacaacgttt ccgtccagga ggtggttctt
    10441 tcgtctcccg aaacctgggc caccgaggag ttcctcgagc gatttgtgga cagacaggtg
    10501 accgagtggt accgaccgat aaccagagac cggcagcccg caacgcctct ttttggatcg
    10561 cttcgggtga tcgtctcgac ggacaggccg cttgccgaag acaggtacca ccactggcac
    10621 atgagccacc gcatttccct gaacagcacg caactgataa ccaacgtggg ccgtggtggc
    10681 acgctggacc tcctccgccc ggaggatatt cggccggagt tccgggacac aattctcgct
    10741 gcgttggacg atgcggcccg gcgaacgatg gaagcgatgg cggcatatga agcaaaagca
    10801 ggcgccagat atacgttgga gacgggatta cccgttggca aagacgcgtc cggtgtctca
    10861 tacggaattc cgcggtactt gatgctggac tttctccttc gtcccgtctt ccaccggaaa
    10921 ggggatgtag tagaaaccgt tcccagaatt gatgagaaag gcaatcgcat cggatctgtc
    10981 ttcatgctgc gggacgggaa tgacgttttc gagggcgaga tcgtcgattg ggaggttatc
    11041 ctgattgagc cgaatatcgg catcggtctt tgggaccgtg tggcgattcg agaagaagag
    11101 cttgaacgaa aacgtgcaca ggcaaccgga cggcccatgg actgggatcg agtgggcgag
    11161 aacgcgcgcg tggttcttcg ggacctgacc cgcgccggta tcgactatct tgaagcgaaa
    11221 cggcgcggta gtacctgaag ctctgaaggt agtgccgacc acgccgagag gggttgccac
    11281 ccggtggaaa ccagattcga acccacgcaa tatctcgttc aagccgccgc agatgcgctt
    11341 ctcgaatacg aaaacggacc ggacctaaca ttcgcggcag gcgactggga ggcagttttc
    11401 ggagcagcat accgctggtt tcgcgcattt ggaagagaat cgctggaaga cctgagccag
    11461 aacgcaccgg attccgttac ggctggcgag aagctcttga acgaatgccc ggaccgcctt
    11521 gccatgctcg tggcggatgc ccttaccaca aactccattg ccccgcctgt gtgcatctca
    11581 ctgacgcctg tacatcttga cactccctat ggggcggtcg tgtttgcagc gttcccgcgc
    11641 gcgatcgaag aactcgcgcc cctcctggtt gacggtctgg aagagcagat gcggtcgcgc
    11701 aggttttccg gcgtggtcgc catccacgac acgtggatgc agaaggatgg gtacccggag
    11761 cgcatactcc gtctcgacgc agagaacaat gtcatcgtcg aacagggcgt tctgaaccca
    11821 tgcaggccgg agagaatact gcttctgctc gggcccgggt cggacgaagc aacgtggcac
    11881 agacggtggc acgatgtcgc cagcaattgc ggcattgatg tggcgaatcc ctacgagttt
    11941 gcatacattg ccgacgacaa atggcagaca ttccacatct tgcagaacgc cggtgtgccg
    12001 acgccggtta cgcgcctcgt gccacctgat acggcgttca ctgcggagat gtgcctcagc
    12061 ctggtggacg gaccgtcggc gcctgttgtc gccaaaccca ggcatggcac ggaaggcctt
    12121 gctgctacgg tgtgcaatcc ggccgaaacg accgacccat tgaaagctat ctgggaacgc
    12181 ggcgacgatg cgttgcttca accgttcacg aacgatctcg tatgggtgga cgaggtggga
    12241 gaagtccaca ccgccgtgct ccgaatgaat gtgacgtgca acgcacaggg tgccagtcgc
    12301 gccgagtcgg gattcgtgca tgtcgcggcg cacggttgtg gtatcgcgtc cgtgggccat
    12361 ggaggcaaat tgagaccgat tctccggtcc gcactgagag tgcgtatcgg cggtcgactc
    12421 gccccgtttc ctgtacacct gacaacacag tgtcaggcgt gtgcgtgcgc ggcagtcgac
    12481 gccgtggcgg ctgcgtcgcg caagacgagg ctcgcaggcg ttgatatagt ggtttctgct
    12541 ggtgacgacg gcgtgccgcg tgcccttgcg ctggaggtca accctcgacc ggcagggctg
    12601 agccacagcc ggtttgtttc agcgggatgg gagacggaag aagaaccggg cgtttcccga
    12661 ctcatgtggg gtgagtgacc gggggcgacg ttctcgaaac gagctgcaca aaaagaggcg
    12721 ctccggagca attccggagc gcctcttttt ttatggcggc aaggagccta acgaagcagc
    12781 gtaacccttc gcacgatccg tacgccac
//
LOCUS       DAOTNG010000052        12770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4728_length_12770_cov_5.413134, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000052 DAOTNG010000000
VERSION     DAOTNG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_12770_cov_5.413134"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5179_03905"
     CDS             <1..229
                     /locus_tag="P5179_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonide lyase"
                     /protein_id="HRS94395.1"
                     /translation="GDVVRIWRLGDQGFGPPRVLCRHRCSSHIQQLHVHPRFNPAGTQ
                     VLFTSDMSGYGNVYLADVPDVGKLPDVEVLR"
     gene            226..1515
                     /locus_tag="P5179_03910"
     CDS             226..1515
                     /locus_tag="P5179_03910"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94396.1"
                     /translation="MKIVLIGAGSASFGRGQVVDVLSAAELQGKHVELCLVDVRQAAL
                     DTMTRFAERVKAHIGSDVIVTATGNRRAALSGADYVMLAVARERAKLWEQDFRVPLAY
                     GFRHCLGENGGPGAVFHALRSFELIIPICHDMEELCPNAWLFNFTNPESRVLHAMKHL
                     TNVKGAGFCHGVFTVLEYLTRILNRPLDDLEVVSAGINHFYCVLRLCDRNTGADLLPH
                     VIQRAASDRNAPPLFRKIAEVFGVITFPSDDHIGEYLSFGTEFSGVKWPYGWESKPVP
                     PVPPTGGPPALEDYASGKVPLDAEILRTSGELTVPAICDMELDRKSSRPAVNVLNTEA
                     YVENLPRTAVVEVPATIGREGITPFHVGAIPEPFASYMRAQTTIVELVTEAYRTKSRR
                     LLLQALLLDPVVNSVSAAERLLDDMLSLQRDYLPRFD"
     gene            1542..2477
                     /locus_tag="P5179_03915"
     CDS             1542..2477
                     /locus_tag="P5179_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRS94397.1"
                     /translation="MQSPDRRELREDVTDAIWEPVFDEAAPTFDADTPKRLKRDRPKW
                     WLHGLLFGLTAWTCYRAAGWYYSIGLMAILLAHEMGHYIMSLRWRVRATPPFFLPFPD
                     IPGVIESPLGTLGAVISMKSPVPSKRALLDIGATGPISGMVVSLVVVVVGLHFSTIEV
                     VPDRLPAGTGLYVFGDSLLFSALRYAILGPVPAGYDVFLHPLARAGWVGFFVTALNLL
                     PIGQLDGGHVLYALHQKHYRLVVRLVTAALIALILVSPVWILFALMAGIFGQTHPPPL
                     DSYTPVDRKRRILGYVCAAILVACFMPDPIDLKVF"
     gene            complement(2516..2857)
                     /locus_tag="P5179_03920"
     CDS             complement(2516..2857)
                     /locus_tag="P5179_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRS94398.1"
                     /translation="MPTLFSRIAAGEIPATIVARTSDCIAIEDINPQAPVHVLIVPLK
                     EIPSVNTLAAEDEALVGKMVLLAREIAAQRGLQEGGYRLVFNCGPDAGQTVDHLHLHL
                     LGGRSFGWPPG"
     gene            complement(2878..3444)
                     /locus_tag="P5179_03925"
     CDS             complement(2878..3444)
                     /locus_tag="P5179_03925"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS94399.1"
                     /translation="MKNLFRRAGLTSIAVAIVIAAAAAGCSKKESDQTASTEISSETS
                     APRFVSMNAPAPDFTLKNRITGADVKLSNYRGRVVMVNFWAPWCPPCREELPDLVGLY
                     EANRGKGFELLGVFVSGDPGEVDQMVRQHGIDYPVLTGDESVARAWLVNGIPTTYIID
                     REGVVRVQYVGAQPRAVFEQDVTRLLNN"
     gene            3689..4624
                     /gene="cysK"
                     /locus_tag="P5179_03930"
     CDS             3689..4624
                     /gene="cysK"
                     /locus_tag="P5179_03930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRS94400.1"
                     /translation="MGRIYANIIETIGNTPLVRINRIMDGAAAEVVAKVEAFNPLASV
                     KDRIGAAMIEAGEKAGKITKDTVLIEPTSGNTGIALAFVCAAKGYRLILTMPESMSME
                     RRALLRALGAEVVLTPAAEGMKGAIKKAEELAKETPHAFVPQQFSNPANPEIHRRTTA
                     EEIWRDTDGKVDILVAGVGTGGTITGISEVIKTRKPSFRTVAVEPAASPVLSGGTAGP
                     HKIQGIGAGFVPDNLNVQIVDEIIPVENDDAMAMARRAAREEGLLVGISSGAALFAAR
                     AVAKREENSGKMIVVILPSFGERYLSTDLFKDLMV"
     gene            4729..5715
                     /locus_tag="P5179_03935"
     CDS             4729..5715
                     /locus_tag="P5179_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94401.1"
                     /translation="MADIGMVGVGGYGATILRAAHEAKNVRLAVVYDVDPERARAAAG
                     KTGAKVAASYEEMLRDDSLQGVILVVPNPLHRRMLEAAVDAGKHVYVEKPIANSVKDG
                     MGMRRYAAAAGRILMVGHNTRRAQTFRTVKHMIDRGELGTVVAMEANFSHAGGLGVPP
                     DAWRYRRETCPAVPLMQLGVHCVDTMNYFAGAPVRVSSFMSSRIMKQNDDVTVTLVEY
                     ENGVLATLHSHYVVAGTNFVAAYGSEANVYAEWERLDKTTREPHGVVSTPFPRNDTQL
                     EEISEFAACIESGAQPETDATAGILALAVVEAAIISHKEQRIVSLEEILQDG"
     gene            complement(5727..6788)
                     /gene="amrS"
                     /locus_tag="P5179_03940"
     CDS             complement(5727..6788)
                     /gene="amrS"
                     /locus_tag="P5179_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRS94402.1"
                     /translation="MHEAMFYERLAGNSVRCQLCHQQCVIADRHHGLCGVRENHGGTL
                     YSVNYGRVIAAHVDPIEKKPLFHFCPGSRAFSVATAGCNFRCAFCQNWDISQASKGEN
                     RSIGGHYTEPQAAVEAALRTSSRVIAFTYTEPTVFYEWALDVARAAAEKDLKTAFVTN
                     GYIRADPLDSIQPFLHAANVDLKAFRDESYRRVMGAALQPVLDTLIRMKRLGIWVEVT
                     TLVVTDLNDSEDELRDIARFISGELGPETPWHVSRFHPDFRYTNVPPTNPHVLKRAHE
                     LGQEEGLHYVYVGNLPGTDGENTRCHKCGNLLVNRIGFHVVENRIATGSTCPQCGERV
                     AGVGMEVVSQRGNSSAGTR"
     gene            complement(6811..7164)
                     /locus_tag="P5179_03945"
     CDS             complement(6811..7164)
                     /locus_tag="P5179_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS94403.1"
                     /translation="MDGREKPKYIVRTTEGLDEVRSTCGFRKSIFTEDDGAGFSVSLL
                     RISDSKKHYHRATWETYYVLEGEGELELDGEMIALSQGTAVLIRPGVRHTARGNVIAL
                     IMGAPPFRTDDMFFD"
     gene            complement(7172..7645)
                     /gene="smpB"
                     /locus_tag="P5179_03950"
     CDS             complement(7172..7645)
                     /gene="smpB"
                     /locus_tag="P5179_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRS94404.1"
                     /translation="MPAGTRIITKNRKAFHDYSILETTEAGIALVGTEVKSLRLGNVN
                     LKDSYARVERGEVVLCGLHISPYDHGNRYNHDPERPRKLLLHRREIGRLKGRSEEKGL
                     TLVPLSLYFKGGRVKVELGLAKGKQIHDKRETLRRREIEMDMEQAIRRQSREQLE"
     gene            7797..8690
                     /locus_tag="P5179_03955"
     CDS             7797..8690
                     /locus_tag="P5179_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94405.1"
                     /translation="MPLLLGALILLCASCSPATSDCLDTLSEIGSSPEIVAASRASVF
                     PDTSCGGLLFSIGTAEYAQASFTAARKAFEAATVFWPDSSKAWNGLGLANYRIRDYSA
                     AERAFKRSLRLAPRQIDVYSNLGLVFLAQARYIDATAAFIAALGINPGYTEARFNLAY
                     TYDLQGRPDLAEGEYYRVLRDHPDHPTARYGLAVLFRKAERFGEALAQLDSALATHPD
                     YADARVERGFLFLRRNEYSRAAGDFRRVLDSHPENAQARYGLGLAYAFQRQWVAAHAQ
                     YESLAVADTTYARRLRRVLGN"
     gene            complement(8759..10039)
                     /gene="eno"
                     /locus_tag="P5179_03960"
     CDS             complement(8759..10039)
                     /gene="eno"
                     /locus_tag="P5179_03960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRS94406.1"
                     /translation="MSMIESVHARQILDSRGNPTVEVEVVLEDGSFGRAAVPSGASTG
                     AFEALELRDGEAAYGGKGVLKAVKNVNETIAPEIEGLDATDQVEIDELMIKLDGTPDK
                     SNLGANAILGVSLAVAKAAAETCGLPLYQYLGGPSARVLPVPMMNILNGGKHAVDSTD
                     LQEFMVMPVGAPTFSEALRWCAETYQALKKVLSAKKYNVSIGDEGGFAPSLKSNEEAI
                     EVILQAIEKAGYKPGDDIMIALDPAASEIFEDGKYVLAKEGRTLSAPEMVDFYERWVA
                     KYPIISIEDGMAEDDWEGWALLTERLTDKIQIVGDDLFVTNTERLQKGIDEEVANSIL
                     IKLNQIGSLTETLAAIEMAKCASYTAVVSHRSGETEDTTIADLVVATNAGQIKTGAPC
                     RTDRVAKYNQLIRIEEALGSVAKFAGRSAFYSIG"
     gene            complement(10191..11141)
                     /locus_tag="P5179_03965"
     CDS             complement(10191..11141)
                     /locus_tag="P5179_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94407.1"
                     /translation="MKTSVASNWLCCVVLAGFVCSCGSSFRFPAEVVRLVLPRTGSEI
                     VGEVQLPARRTFAPVPLVVLVDDGPPIDIDPRRTQANQIQQLQAALVSNGYAVWRPLR
                     DAWPSDSLVVRCPDKLTSQVLLGFLSARNIRGIDTARVVFLGFGQGGVIAALAAERSA
                     GRVHALGLVNTPARSVDRTLLSGTFRDSVNLRRLEQFFDDIWTDKYNDSDFVMNGRVE
                     CWRSWVVLSGEMTDRVARLSVPTLAVQGTADSLLPLLDIERFRRAIGRRQLSQVHTAV
                     GLRHDLRDEIPDPGQDKKAISPRFVSIVLEWLKLVAPPAG"
     gene            complement(11184..12476)
                     /locus_tag="P5179_03970"
     CDS             complement(11184..12476)
                     /locus_tag="P5179_03970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRS94408.1"
                     /translation="MSVTVAVGTHWGDEGKAKVVDFLTEKNDIVVRFQGGANAGHTVI
                     ADGETWIFHQIPAGILHPGKVCVVGNGVVLDPLAFLEELDHVRGRGVSVEGRLWISDR
                     AHLVMPYHKQLEGIQEAARGTGAIGTTKRGIGPTYYDKISREHGLRAGDLLDAMGFRA
                     RLRKIAADKNEVFTRVYGAEPVNADAVADEYLGYAEQMRPFIADTATILHAAQNAGKA
                     ILCEGAQGTLLDIDHGTYPFVTSSNTSAGGACTGTGIGPTAISEVIGIVKAYTSRVGN
                     GPFPTEFECEMGNRMRELWNEYGATTRRPRRCGWLDGVILEYSARINGLTGLAVTALD
                     RLDALSELRICREYVLDGTPLHTVPARTEDFDRVKAVYDTLPGWETDTTSARCFEELP
                     ENARRYIRRIEEIAGVPVRYVGIGPERDQMIVVRSQTV"
BASE COUNT         2606 a         3886 c         3562 g         2716 t
ORIGIN      
        1 cggcgacgtg gttcgcatct ggcggctcgg ggaccagggt ttcgggccgc cgcgggttct
       61 atgccgccac cgttgcagca gccacatcca gcagctccat gtgcacccga ggttcaaccc
      121 cgcgggcaca caggtgcttt tcacaagcga catgtcgggt tacggaaacg tgtacctggc
      181 tgacgtgcca gatgtcggca aactccccga tgtggaggtt ctccgttgaa aatcgttctc
      241 atcggtgcag gaagcgcctc gttcggtcgc ggtcaggtgg ttgacgtgct gtctgccgcg
      301 gaattgcagg gaaaacacgt tgagctctgt ctcgtagacg tgcgtcaggc agccctcgac
      361 acaatgacgc gcttcgcgga gcgtgtgaaa gcgcacatcg ggagcgacgt gatcgttacg
      421 gccactggta atcgccgcgc agccctgtcc ggagccgact atgtgatgct tgccgttgcc
      481 cgggagcgtg caaagctctg ggaacaggac ttccgcgttc cgctggccta tggcttccga
      541 cactgtctgg gcgagaacgg aggaccgggc gcggtgttcc acgcgttgcg gagctttgaa
      601 ttgatcatcc cgatctgtca cgacatggaa gagctttgtc cgaacgcgtg gctgttcaat
      661 ttcaccaacc cggaatcccg tgtcctgcat gcaatgaaac atctgaccaa cgtaaaaggc
      721 gccgggttct gccacggagt gttcaccgtc cttgagtacc tgacccggat actcaatcgc
      781 ccgctcgatg atctggaagt agtatcagcc ggaatcaacc acttctactg cgttctgaga
      841 ctttgtgaca gaaacaccgg tgctgatctg ctgccccatg tgatccaacg ggccgcatcg
      901 gatcggaatg cgccgccgct gttccgcaaa atcgcggaag tgttcggggt gatcaccttc
      961 ccgtccgatg accatatcgg cgagtatctg tccttcggca ccgagttcag cggtgtcaaa
     1021 tggccctacg ggtgggaatc aaaaccagtt cctcccgtgc caccgaccgg ggggccaccg
     1081 gcgctagagg actacgcatc gggcaaagtg ccccttgatg cggagattct tcgaacatca
     1141 ggggaactta ccgttcctgc aatctgcgac atggaactcg acagaaaatc ctcccgaccg
     1201 gcggtcaatg tgttgaacac agaagcttac gtggagaacc tgccgcgaac agcggttgtc
     1261 gaagtgccgg caaccatcgg tcgtgaaggt atcacgccgt ttcacgtcgg agccattcca
     1321 gaaccgttcg ccagctatat gcgggcccag acaacgatcg tggaactggt gaccgaagcg
     1381 taccggacga aatcccgcag gctgttgctt caagcgttgt tgctcgaccc ggtggtgaac
     1441 agtgtgtccg ccgcggaacg attgctggat gacatgctgt ccttgcagcg ggactacctc
     1501 ccgcgttttg actagtcacc ggggaagaag ggcgcgtttt tatgcaatcg cccgatcggc
     1561 gcgaactgcg ggaagacgtc accgacgcca tctgggagcc tgttttcgat gaggcggctc
     1621 ccacgtttga tgcagacaca ccgaaacgtc tcaaacggga tcggcctaag tggtggctgc
     1681 atggccttct atttggtctt accgcatgga catgctatcg cgcggccgga tggtattaca
     1741 gcatcggatt gatggcaatc ctcctcgcgc atgagatggg gcactacatc atgtcactca
     1801 ggtggcgcgt gcgggcgact cctccctttt ttctcccatt cccggacatt ccgggcgtaa
     1861 ttgaatcccc gctcggaacg cttggcgccg tgatttccat gaaaagcccc gtcccgagca
     1921 agcgtgccct tctggatatc ggcgcaaccg gtccaatctc cggaatggtg gtgtcactcg
     1981 tagtggtggt ggtcgggctc catttttcaa cgattgaggt tgttcctgac cggcttccgg
     2041 caggtaccgg cttgtacgtg tttggcgatt cgctgctttt ttccgcactg aggtacgcga
     2101 tactcgggcc ggttcccgcg gggtacgacg tgttcctcca tccccttgcc cgcgccgggt
     2161 gggtgggctt tttcgttacc gcgctgaacc ttcttcctat cgggcagctg gacggcgggc
     2221 acgttctcta cgcgctccac cagaagcact atcgccttgt cgtgcgcctc gtcaccgccg
     2281 ccttgatcgc cctcatcctt gtgtcgcccg tctggattct ttttgccctg atggcgggaa
     2341 tcttcgggca gacgcatcca cctccgcttg attcgtacac acccgttgac aggaagaggc
     2401 gcattctggg gtacgtgtgc gctgccatcc tcgtggcgtg tttcatgcct gaccctatcg
     2461 acctcaaagt cttctgacag gctggaatgg cacggcagca cccggggtcg gccgttcagc
     2521 cgggcggcca gccaaatgag cgcccgccga gaagatggag atgcaggtga tccaccgtct
     2581 ggcctgcgtc cggcccgcag ttgaagacca gccggtatcc cccttcttga agcccccgct
     2641 gagctgcgat ttccctggcg agaaggacca ttttgccgac aagggcctcg tcctctgccg
     2701 ccagcgtatt taccgacgga atctccttca atggaacgat cagcacgtgc accggcgcct
     2761 gggggttgat atcctcgatg gcaatgcagt cagacgttcg ggctacgatg gttgccggta
     2821 tttcgccagc agcgattcgt gagaaaagcg ttggcatctg tccctcctgg ctcccgttca
     2881 gttgttcaac aatctggtca catcctgttc aaacaccgca cgcggctgcg caccgacgta
     2941 ctggacgcgc acgactccct ctctgtcaat gatgtacgtc gtcgggattc cgttgaccaa
     3001 ccatgcgcgt gccacgcttt catcgccggt aagaaccggg tagtcgattc cgtgttgacg
     3061 aaccatctgg tcgacttcac cagggtctcc actcacaaat acgccgagga gttcaaatcc
     3121 tttgcctcgg ttcgcttcgt aaagccccac aaggtccggc aattcctcac ggcacggcgg
     3181 gcaccagggc gcccagaagt tcaccatgac tactcgaccg cggtagttgc tcaatttcac
     3241 atctgctccg gtaatgcggt tcttcagtgt aaaatccggc gcaggggcat tcatgcttac
     3301 aaatcgtggg gccgatgtct cgctgctgat ttcagtgctg gcggtctggt cactctcctt
     3361 tttggagcac ccggccgctg ctgcggcaat aacgatggca actgcgatgg aagtcaaacc
     3421 tgcacgtctg aaaaggttct tcatttcgat acccatctct tcgagtccac ccgtgtgtgt
     3481 ttcggtactc ctgcgtggag tcgcaaaatg cgcaagatga aaaacactgg aaccgcagcg
     3541 ttggttcatg cgccaacggc attgcaaaag aatacggtgt gtgcggggca aagaaaagtt
     3601 gacaggctcg cgggaaccgt aagaagcccg gatttgttga tttccaatac gtggtcacgg
     3661 tcgtttccca cattgcggag gtacccagat ggggcggatt tacgcgaaca ttatcgaaac
     3721 gattggcaac acgccgcttg ttcggatcaa tcgcatcatg gacggagctg ctgccgaggt
     3781 ggtagccaag gtcgaagcgt tcaaccctct ggcgagcgtg aaagatcgaa tcggtgcagc
     3841 aatgattgaa gctggcgaga aagctggcaa aatcaccaag gacacggtgc tgattgaacc
     3901 gacatctgga aacacgggca tcgccctcgc gttcgtgtgt gccgcaaaag ggtatcgtct
     3961 catcctcacc atgcctgaaa gcatgagcat ggaacgccgc gcgcttttgc gggcgctggg
     4021 cgcggaagta gttcttaccc cggccgcaga aggcatgaaa ggggcgatca agaaagccga
     4081 ggaactggca aaggaaacac cacacgcgtt cgtaccgcag cagttctcaa atcctgcaaa
     4141 cccggaaata caccggagga cgaccgcaga ggaaatctgg cgtgacactg acggcaaagt
     4201 ggacattctg gtcgcgggcg taggcacggg tggtaccatc accggcatct cggaggtaat
     4261 caagactcga aagccatcct tccgcaccgt ggccgttgag cctgcggcgt ctccagttct
     4321 ttctggaggc accgcaggac ctcacaaaat acagggaatc ggagccggtt tcgtaccgga
     4381 caatctcaat gtgcagattg tcgacgagat catcccggtg gagaacgacg acgcgatggc
     4441 aatggcgcgc cgggcggcac gggaggaggg cctcctcgtc gggatttcct cgggcgcggc
     4501 cctttttgcc gcacgtgccg tggcaaagcg ggaagagaat tccggcaaaa tgatagtcgt
     4561 catattgccc tcgttcggag aacggtatct gagcaccgac ctcttcaaag acctaatggt
     4621 gtaaatcgtc cagcgcgctt gctgtccggt ccggcaattc ctaccttacg cggaggggac
     4681 acacgtctcc tccgcttgtt cattcatcgc agacgaggtg cgaggcacat ggctgatatc
     4741 gggatggttg gtgtgggtgg gtacggtgcg acgatcctcc gggccgcaca tgaagcgaaa
     4801 aacgtgaggc tcgcggtcgt ctatgatgtc gaccccgaga gggcacgagc ggcagccggg
     4861 aaaaccgggg cgaaggtggc agcgtcgtac gaggagatgc tgcgcgatga ctccttgcag
     4921 ggcgttatcc tggttgttcc caaccctctc cacaggagga tgctcgaagc cgccgtggac
     4981 gccggaaaac atgtatacgt ggagaagccc atcgcgaaca gcgtgaagga cggcatggga
     5041 atgcggcgct atgcggcggc agccgggcgg atcctgatgg tgggacacaa tacgcgtcgc
     5101 gcgcagacat ttcgaacggt caagcacatg atcgaccgcg gagaactcgg gacggtggtt
     5161 gccatggagg cgaacttctc ccacgccggc ggcctgggtg tcccgcctga cgcgtggcgt
     5221 taccgccggg aaacttgtcc cgccgtacct ttgatgcagc tcggagttca ctgcgtcgac
     5281 acgatgaatt acttcgccgg ggcgcccgtc cgcgtcagtt cattcatgtc ctccagaatc
     5341 atgaaacaga atgacgacgt gacggttacc ctcgtggagt acgaaaacgg tgtgctggca
     5401 acgttgcaca gtcactatgt ggttgccggg acgaacttcg tggcggcgta cggatcggag
     5461 gcaaacgtat acgccgaatg ggagcggctt gacaagacca cgcgcgaacc ccacggagtt
     5521 gtgtccacac cattcccgag aaacgacacg cagctggagg aaatcagtga gtttgccgct
     5581 tgcatagaat ccggagcaca gccggaaacc gacgccacag cgggcatact tgctctcgcc
     5641 gtagttgaag ctgcgatcat ctcacacaag gaacaacgca tcgtgtcgct ggaagagatt
     5701 ttgcaggacg gataaccggc acgaaatcaa cgggtaccgg cgctcgaatt cccccgctga
     5761 gagactactt ccatcccgac gccggcaacg cgctctccgc attgggggca ggtgctacca
     5821 gtcgcgatcc ggttctccac gacatggaat ccgatccgat ttaccagaag attgccgcac
     5881 ttgtggcacc gggtgttctc cccatccgtt cccggcagat tacctacgta cacgtaatga
     5941 aggccttctt cttgccccaa ctcatgcgcc cgcttcagta catgcggatt cgtgggtggc
     6001 acgtttgtgt accgaaaatc cggatggaat cggcttacgt gccacggagt ttccgggcca
     6061 agttcacctg aaatgaagcg cgcgatgtct cgaagctcgt cctccgaatc attcaaatcc
     6121 gtgaccacga gtgtggtaac ctcaacccag atgcccagtc tcttcatacg aatcagggtg
     6181 tcgagcacgg gttgaagagc ggctcccatc acgcggcggt acgattcgtc tctgaatgcc
     6241 tttaggtcca cgtttgccgc gtggaggaac ggttgtatgg aatcaagcgg atctgccctg
     6301 atatacccat ttgtgacgaa cgcagtcttg aggtccttct ccgccgccgc ccgtgccaca
     6361 tccagagccc attcatagaa cactgttggt tccgtgtacg tgaaagcgat cacgcgagat
     6421 gatgtgcgta gcgcggcctc cactgcagct tgtggttcgg tgtaatggcc accaatggaa
     6481 cgattctccc ccttgctcgc ctgcgagata tcccagttct gacagaaggc acaccgaaag
     6541 ttgcatcctg cggtcgccac agaaaatgca cgggacccgg ggcagaaatg gaacaacggc
     6601 ttcttttcga tggggtcgac gtgcgccgcg atcacgcggc catagttgac ggaataaagg
     6661 gtgccaccgt ggttttcacg gactccgcat aatccgtgat gccggtccgc tatcacacac
     6721 tgctggtggc acaactggca acgaacagag ttgcccgcaa ggcgctcgta gaacatcgcc
     6781 tcgtgcactg cacacttccc cgcgaacgtg ctaatcgaaa aacatgtcgt ccgtgcgaaa
     6841 cggaggcgcc cccataatga gagcgatcac gttcccgcgt gcggtgtgcc gcacgccggg
     6901 acgaatcagt accgcggttc cctgcgagag ggcgatcatc tccccgtcaa gctccagctc
     6961 cccttcacct tccagcacgt agtatgtttc ccacgtggca cggtggtagt gcttcttcga
     7021 atcggagatt cgaagaaggg agaccgagaa accggcgcca tcgtcctccg tgaaaatcga
     7081 tttccggaag ccgcaggtgg acctcacctc gtccaaaccc tcggttgtcc gtacgatgta
     7141 ctttggcttt tcgcgcccgt ccatttcctg atcactccag ttgctcgcgg ctttgccgac
     7201 gtattgcctg ttccatgtcc atctctattt cccgtcttcg aagcgtttcc ctcttgtcgt
     7261 ggatctgctt tccttttgcg aggcccagtt ccacttttac cctgccgcct ttgaaataca
     7321 acgacagcgg cacgagtgtc aagccttttt cctctgaacg acccttcaag cgtccgatct
     7381 cacgtcggtg gagaaggagc ttgcgaggac gttcggggtc atggttatac cggttcccgt
     7441 gatcgtacgg gctgatatgc agcccgcaaa gaacgacctc gccacgttcc acgcgggcgt
     7501 agctgtcctt caggttcaca ttgcccagcc ggagcgactt cacttccgtc cccaccagag
     7561 caattcccgc ctcggttgtc tcaagaatgg agtaatcgtg aaacgctttg cggtttttcg
     7621 ttatgattct tgttccggcc ggcattctgt cactccggtt ttttcgagaa cttcacacca
     7681 aaagaacgta accaaagtct tgttccaaaa cattcggtcg cactccctgt tgccgcaaca
     7741 catgacttgc gtactatttg tgcaaaggcc ggctctgatg tgattaggga gggaagatgc
     7801 cacttcttct cggtgctctg atactgttgt gtgccagttg ttcgcccgcc acttcggatt
     7861 gtctggacac gctgtcggaa atcggaagct ccccggaaat cgtagccgct tcccgtgcct
     7921 cggtgttccc ggacacttct tgcgggggcc tcctcttttc gatcggaacc gccgaatatg
     7981 cccaggcttc attcaccgcc gcccggaagg catttgaggc tgcgaccgtt ttctggccgg
     8041 attcctccaa agcatggaac ggacttggac tcgcaaatta ccggatccgt gactattcgg
     8101 cagccgagcg cgccttcaaa cgatcgctgc gccttgcgcc gaggcagatc gatgtataca
     8161 gcaacctcgg cctcgtattc ctcgctcagg ctcgttacat tgacgcaacg gcggctttca
     8221 tcgccgcact gggcataaac ccgggataca ccgaggcacg gttcaatctc gcgtacacct
     8281 acgacctgca agggaggcct gaccttgccg aaggcgagta ctaccgcgtg cttcgcgacc
     8341 accccgacca tccaaccgcg cgatacggac tggctgttct tttccgaaag gccgaacggt
     8401 tcggtgaagc gctggcccag ctcgattccg cgctcgcaac tcacccggat tacgcggacg
     8461 cgagagtgga acgggggttc ctgtttttgc ggcgaaacga gtattcccgt gctgcggggg
     8521 acttccgtcg cgtgctcgat tcccacccgg aaaatgctca ggcgcggtac ggcctgggac
     8581 tggcgtacgc gtttcagcgc cagtgggtag ccgcgcacgc gcaatacgaa agcctcgcgg
     8641 tggccgatac gacgtacgcc cggcgcctgc gccgtgttct ggggaactga gcccataaaa
     8701 aggagaggag cacaacgcgc ccctctcctt tgctccagaa caatactcag ttcgcgattt
     8761 acccgatgct gtagaacgcg cttctcccgg caaacttggc aacgcttccc agcgcttctt
     8821 cgatccggat gagttggttg tacttcgcta cgcgatcagt tctgcagggc gcgccggttt
     8881 tgatctgacc ggcattggta gccaccacca gatccgcaat cgtggtatcc tcggtctcgc
     8941 ccgatcgatg gctcaccacc gctgtgtacg atgcgcattt ggccatctcg atcgctgcga
     9001 gcgtctcggt aagcgacccg atctgattga gttttatcag aattgaattc gcgacctctt
     9061 cgtcgatccc cttctgcagc ctctcagtat tcgtgacgaa aagatcgtcg ccgacaatct
     9121 ggatcttgtc ggtcaaacgt tccgtgagaa gtgcccaacc ctcccagtcg tcctcagcca
     9181 tcccgtcctc tattgagatg atcgggtatt tggcaaccca gcgctcgtaa aagtccacca
     9241 tttccggcgc ggaaagcgtc ctgccctctt tggcgagaac gtatttgccg tcttcgaaga
     9301 tctcgctggc agcggggtca agggcaatca tgatgtcatc gccgggcttg taacccgctt
     9361 tttcgattgc ctgtagaatc acttcgatgg cttcttcgtt cgacttcagc gaaggggcaa
     9421 atccgccctc atcgcctatg gacacgttgt acttcttcgc gctgagcacc tttttgagag
     9481 cctggtacgt ctcagcgcac caccggagcg cctcactgaa cgtaggagcg cccaccggca
     9541 tcaccataaa ctcctgaagg tccgtcgagt cgacggcgtg tttcccgccg ttcaggatgt
     9601 tcatcatggg caccgggaga actcgagcgg acggtccacc gagatactgg tagagtggca
     9661 ggccgcaggt ttccgccgct gcctttgcca cggcgagaga aacgccaaga atggcgttcg
     9721 ctcccagatt gctcttatcc ggagtaccat cgagtttgat catgagttcg tcgatttcca
     9781 cctgatcggt ggcgtcaagt ccttcgattt caggcgcaat cgtctcgttg acatttttga
     9841 ccgccttcaa aacgcccttg ccgccgtacg cagcctcgcc gtcgcgcagt tccaaagcct
     9901 caaatgctcc cgtggaagcg cccgatggca ctgccgcacg gccgaacgaa ccgtcttcaa
     9961 gtaccacttc aacttcgacg gtcgggttgc cgcgcgagtc cagaatctgg cgggcatgaa
    10021 cactctcgat catgctcatg gcgtatcacc tttctcgtgt atcgatggga acgaacgctg
    10081 ttcacaaaag caatgttgtc tctgtgaacc agctgctgat tgtgctgcaa cgcaaacaac
    10141 gtgagtccgc ccgatcctgg agcacgtctt tattcgtcaa agacgatctc tcacccggca
    10201 ggcggtgcca caagtttcaa ccattcaaga acgatggaca caaatcgtgg gctgatagcc
    10261 tttttgtctt gaccgggatc cggaatctcg tcgcgcagat cgtgtcggag ccccacagcc
    10321 gtgtggacct gagaaagctg ccgtcgccct atcgctcgtc gaaaccgttc aatgtccagc
    10381 aagggcaaca aactgtccgc agtgccctgc acggccagcg tcggaacact gagcctcgcc
    10441 acgcggtcgg tcatttcgcc cgagagcaca acccaggatc gccagcattc cacacggccg
    10501 ttcattacaa aatccgagtc gttgtactta tcggtccaga tatcatcgaa aaactgctcc
    10561 aaccggcgga ggttcaccga atcgcggaac gttccggaga gaagcgtcct gtccaccgaa
    10621 cgagccggtg tgttcaccag tccgagcgcg tgcacacggc ccgcggaacg ctcggcggcc
    10681 agtgccgcga taaccccgcc ctgaccgaac ccgaggaaca ccaccctggc cgtatcgatt
    10741 ccccggatat ttcgcgcgct tagaaacccg agcagtactt gcgaggtcag tttgtcaggg
    10801 caccgaacta cgagactatc ggaaggccac gcgtcgcgca gaggcctcca cacggcgtat
    10861 ccattggaaa caagtgcggc ctgcagttgt tgaatctgat tggcttgtgt cctgcgggga
    10921 tcgatatcta ttggcggtcc gtcatcaacg agaactacaa gcggcaccgg cgcaaacgtc
    10981 ctccgcgccg gcaactggac ctcgcccaca atctcacttc ctgtccgggg cagaaccagc
    11041 cgaaccactt ccgccggaaa acggaaagac gagccgcacg agcatacaaa accggccaga
    11101 acgacacaac acagccagtt cgaggccacg ctcgttttca tacgttccga cgcctcggtg
    11161 tgcatacctg ggattctccc cgttcacaca gtctgcgacc gcactacaat catctgatcc
    11221 cgttccgggc caattcccac ataacggacc ggaacacccg cgatctcttc aatccgccgg
    11281 atgtaacgcc tggcgttttc cgggagttcc tcgaaacacc gtgcggaagt agtgtcggtt
    11341 tcccagccgg gcaacgtgtc atacaccgcc ttcacacggt cgaagtcctc cgtgcgcgca
    11401 ggaaccgtgt gcagaggtgt tccgtcaaga acatactccc ggcagatgcg taactccgaa
    11461 agagcgtcca ggcggtccag cgccgtcact gcgaggccgg tcaacccgtt gatacgcgct
    11521 gaatactcaa ggatgactcc gtccagccag ccacaacgac gtgggcgccg cgttgttgct
    11581 ccgtactcat tccagagctc gcgcatccgg tttcccatct cgcattcgaa ctcggtcggg
    11641 aagggaccgt tacccacgcg actcgtatac gcctttacaa tgcctatgac ttccgaaatc
    11701 gcggtcgggc caatccccgt gcccgtgcac gcaccacccg ccgatgtgtt cgagctcgtc
    11761 acaaaggggt acgtaccgtg atcaatgtcg agaagggtac cctgggcccc ttcacatagg
    11821 atcgctttgc ctgcgttctg ggctgcgtgc aggatcgtcg cggtatccgc gatgaacggc
    11881 ctcatctgct ccgcgtatcc gaggtattca tcagccactg cgtcggcatt caccggctct
    11941 gcgccataca ccctggtgaa cacctcgttc ttgtccgccg caattttccg cagacgcgcg
    12001 cggaacccca tcgcatcaag gaggtccccc gcgcgaagtc cgtgttcacg ggaaattttg
    12061 tcataatagg tcggaccgat tccgcgcttc gtggtcccga tggcgccggt ccctcgagcc
    12121 gcctcctgaa taccctcgag ttgcttgtgg tacggcatca cgaggtgtgc gcggtcgctg
    12181 atccacaggc gtccctccac cgaaacgccc cgtccgcgga catggtcaag ctcctcaagg
    12241 aaggccaacg ggtccaacac aacgccgttg cccaccacgc acacctttcc gggatgcagg
    12301 attccggcag gaatctggtg gaagatccac gtctctccgt ccgcaatcac cgtatgcccc
    12361 gcattcgctc cgccttgaaa acggacaacg atgtcgttct tctccgtgag gaaatcaacg
    12421 acctttgctt tcccttcgtc gccccagtga gttcccacgg caacagttac cgacatggcg
    12481 ctatcctttt ctcagatcac cctgaacgag gcattgcctc ggagagatgt atccaaacac
    12541 gcgcaaaacc gcgcggcgga gtcacctcca cgcagaaacg aaaaaacgac ccagccgcca
    12601 cgccggcggt tgagccgtac ctgaatatat ctgaacggct tcctctagtg caactatgcg
    12661 tacgtgtaca cgcgtgcacg caattgcatg tgccggctgg acgtctgaac gccggtttcg
    12721 ctacattgag gattccctca atcatggaac acctcacaga ggtctgcccg
//
LOCUS       DAOTNG010000053        12614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4785_length_12614_cov_4.667410, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000053 DAOTNG010000000
VERSION     DAOTNG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_12614_cov_4.667410"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="P5179_03975"
     CDS             <1..1470
                     /locus_tag="P5179_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS94409.1"
                     /translation="AGDRILPTPWTRAPLLIRVSAPHRESAQIVIGYSARHVDRLIED
                     TVRPAVLDEKSGAWRILTSASVNIRARTITSQFVQSGLWTICGLSRVRWQAPQRESNV
                     TVGDIDGDGRLEVALTQYANAELLNSDGSSRHVFQLQASNRPVQNTSSPLIADINADG
                     RNEIVYGATSGHVHAFSPDGVELWRTPVGGEVRGGIAAAMSDDGRPSCLCVSWHDAGV
                     ALLNPDGSVRWQHSLTPPADTTPVVCIPRAGGPFVVVAHGEGLIAFRCADGEVLWQYR
                     TEGYSPVAPAVGEFSRSEEPHIVTGTASGMIYVLDAHGEVRSRWVVPAVHEAYRPIIE
                     VGMASLRDSSTRQIVATTAGGVVVAYERDGTPLWQFVSREQVAGFALGVGGRLAFADV
                     NADGNDEVLVAEQDQYVYSLKGDGSLLWEFRGAFFYHYSPVVADLDGQGDLTLLTTSP
                     CQNGTYALRAGHRVAGASRTPWPTMRGNFARTNCAPWPR"
     gene            complement(1537..1800)
                     /locus_tag="P5179_03980"
     CDS             complement(1537..1800)
                     /locus_tag="P5179_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94410.1"
                     /translation="MDGQVERFGFRGRSGRSLDARMSYRQFAEMAELHRRLVGQVDQR
                     VGREKAILRKYIAAIDVALRNHPDANEFFARWRKESGMSEREA"
     gene            1896..3146
                     /gene="lysA"
                     /locus_tag="P5179_03985"
     CDS             1896..3146
                     /gene="lysA"
                     /locus_tag="P5179_03985"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRS94411.1"
                     /translation="MHYFAYANEVLRGEEVDLRDIAESVGTPCYVYSHRTLLQHAHVL
                     DAAWGEREHLTCYSVKACSNIAILRLFAQMGLGADIVSGGELYRALLAGFPPDKIVYS
                     GVGKTPEEMREALRAGIFCFNVESEAELVELNRVAQREYRRARISLRINPEIDPKTHP
                     KTATGLKTAKFGVPHSRAVETYRRASELEWLEVIGIDAHIGSPIMSVQPFVDAAKRLV
                     DLVGHIRAEGIELKWIDIGGGLGITYDVEDPPTPKEWATAVGDVLNDAGLGIITEPGR
                     SIVGNAAVLLTKVLYLKRNEDKNFVVVDAAMNDLIRPSFYDSFHRIQPVVQRGTEPFV
                     ADVVGPVCESGDFLARNRSIARPEQGDILAVMSAGAYGFAMSSQYNSRRRAAEVMVKG
                     GTWQVIRDRETCEDLVRGERFFTL"
     gene            3121..4173
                     /gene="thiL"
                     /locus_tag="P5179_03990"
     CDS             3121..4173
                     /gene="thiL"
                     /locus_tag="P5179_03990"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRS94412.1"
                     /translation="MANAFSPSETRGDTVNADGMTEADLLKTLFGTLPPCGADVIVGV
                     GDDCAVLRADGDDVQVLTTDALIEGVDFVVGPGFSFENLGHKALAVNLSDIAAMGAIP
                     RHALVTLCIPEHYGRKEMAELYQGFSRLASRHGVGVIGGDLSSSRNDFMVSVTLLGTA
                     SRSEILLRSGAKPGDAAMLFGRIGDSGAGLDITRGAHCPSEAILLERHFRPEPLVDQG
                     RWLANRTGNHVHAMIDISDGLASDARHICEASGVGVRIDHASLPVSDELAEFARYTGR
                     ELDGYLLGAGEDYALFAAVDASAATEIAGRFRSAFPRTPVATVGSFRRGNHVEIRWEN
                     GAVSELAGGFDHFARQ"
     gene            4185..>4518
                     /locus_tag="P5179_03995"
     CDS             4185..>4518
                     /locus_tag="P5179_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94413.1"
                     /translation="MPDQKIDVVRMSFEEERASAYLYRAMESVETGAAAAMFGKLRKA
                     SEAQSEIWAEQLSSLGATVPAFRPSLRVRVVRWLLFRLGPRRMLPVLAGMKVRGLSIY
                     STRGATPST"
     assembly_gap    4519..4528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4529..5258
                     /locus_tag="P5179_04000"
     CDS             <4529..5258
                     /locus_tag="P5179_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRS94414.1"
                     /translation="EEALASPSTAGEKWHRAAQGGGALRAAVFGMNDGLVSNASLIIG
                     VAGGAADQPEVVLLAGVAGLIAGGFSMAAGEYISVATQKELYEHQIALEKAEMAVFPE
                     EEIEELAMVYETKGLPRDQAVAVATRIVNDPVNGLDTLAREELGLDPAGLSSPVAAAS
                     ASFAAFAVGALIPLMPYLVTRAPATLSLTVVVTEVALLLVGAVMSLFTGRNMLWSALR
                     MALIGSAAAAITFAIGRLLGVSIS"
     gene            5300..6334
                     /locus_tag="P5179_04005"
     CDS             5300..6334
                     /locus_tag="P5179_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRS94415.1"
                     /translation="MEWEMVVNPETIVGEAHASTLIESPGGDLLCAWFGGAPEGSPGT
                     AIWMSRRTSSGWSPASVIADEPGVPCFNPVFFRHGTTIWVYFKAGKEVEKWSGFRTRS
                     EDGGFTWSAPEILPAGLFGPIKNKGIRLHNGMIAFPSSAESYRAWTGWVYLHDPDSDA
                     WKIRGPLAHPDNPWGVIQPTLWEVTPGHIRALLRSRKEIGRVCVSDSFDSGETWTPAR
                     PSSLPNPNSGIDAVKLADGRVVLAYNHTDPSPRLSAATGDMPGNTPLLGGRHEIHLAV
                     SEDGGETWSPPWEMEIGGAEFSYPAIIQSSDGLVHVTYTWNRKGIRYGAFTPSEISLI
                     CSGTFPRPAP"
     gene            6345..6917
                     /locus_tag="P5179_04010"
     CDS             6345..6917
                     /locus_tag="P5179_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94416.1"
                     /translation="MCGMTGHEHRGVRENSRRRLDPVEEKILLLLAGRAEDMVPLVDI
                     TTDLAGLPDHLRGQWFDKKLLTPSQVAAWEEALKQVRRAVRSLFRKGFIDVGREDEFP
                     EGFCRHHAEVIRALQNHPAWKGDTDTSKRSVAGIESEIRARMPWADARDLTAVQKQMD
                     GPVDIVGITSSGRAELTSNPGQSLAGSQPF"
     gene            6895..8514
                     /locus_tag="P5179_04015"
     CDS             6895..8514
                     /locus_tag="P5179_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS94417.1"
                     /translation="MLVLSLSDISVPFGESEDALRALCAARLGVPEAELGQLRVRRRA
                     LDARHPSRLRYVYTIEVEAPLTLEQARRISGAKVVDSTLARRVEFGTRALLERPVVVG
                     AGPAGLFAAWKLAENGYRPILIERGKPVEARRRDATDFWKYGRLDPDSNVLFGEGGAG
                     AFSDGKLTSRSKDWRRDDVLDRLARCGAGDSVRFEARPHVGTDRLFYVLKEMRATLSA
                     LGTEILFETRVEDFLIDRGTLLGVRCAPDVVIPTNALILACGHSARDTYHALRARGVA
                     LEQKPFAMGIRVQHRQEIIDRAQYGRRAVSHEGRDRSLQPAEYVLRCPKTSTGRSVFS
                     FCVCPGGAVIPCASEPGGLFCNGMSGRNRGGRFANGAIVAQIPTADFASADPLSGIDF
                     QRHWERAAFKSTGGTYALPMMPLVSFVDGSWPPRRVHGLAKGLFPRADLVDLTSLLPP
                     SVVQAVREASHEFSKTIHGWLAPDAVAFGVETRTSSPVRILRREDGQSENVAGLFPAG
                     EGAGYAGGIVSAAVDGMRAAEHLMATFAPPS"
     gene            8843..9514
                     /locus_tag="P5179_04020"
     CDS             8843..9514
                     /locus_tag="P5179_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS94418.1"
                     /translation="MRVLLVEDDSRIAHFIQKGLTESGYVVDLVADGVEAVSQVASTA
                     YDVIVLDVMLPRMDGFGVLESLRQQGQKMPVLMLTARDTTDDKVRGLDAGADDYLTKP
                     FSFAELEARLRALLRRGTGEASAHLRLGDITVDPVSHVVKRGDTVIEMTPKEYAVLEY
                     FMRYPGRVLTRTMIIEHVWQYQFDTDTNLVDVYINRLRRKLNDAEGRILQTIRGVGYT
                     LRPAQ"
     gene            9511..10977
                     /locus_tag="P5179_04025"
     CDS             9511..10977
                     /locus_tag="P5179_04025"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS94419.1"
                     /translation="MKSRSLRFELTFWYSTVMAVTLLVMGVAVEQVAYNRISASIDNS
                     LRQAAFTVISEVGVLRSKPDAEPFGETSTVLNPPPWPPRYVQILSASGEVLYRSANLG
                     QYPLPIDSAALRDRGRGMVISPDMRLHGEPIRMVTFPLPEYNGSNAAWGQVALSLHDL
                     DRARKKNQLALAIILPAAIGISAVAGWWLARRALRPIDGVIRTAQRIRAANLNERIAP
                     RDVDDELGRLISTLNELLERLELNFRQVSRFSADVSHELRTPLTIIQGEAEVALREGA
                     SREEMQLALEVALDEAQRMSKLVKNLLMLARLETGQERPHFVETPLAPIIEDVSEEGL
                     ALARAKGVTFSAHTVENASVWGDAVLLHQLAFNLVDNAVKYTPAGGTVELSLSRNGSS
                     VRLSVKDTGVGIDEAELGRIFDRFYRGDSARNHTEGGSGLGLALVAQIAEAHKGTIQV
                     TSKPGEGSVFTVTFPLASQAGKARPDMAEVSMIEGIRS"
     gene            10974..12404
                     /locus_tag="P5179_04030"
     CDS             10974..12404
                     /locus_tag="P5179_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRS94420.1"
                     /translation="MIETAVILAAGNGSRLRGVSAGLPKPLVPLAGVPIIVRVMRAAQ
                     EAGIRKFVVVTGYQAEKLRAAIENQPAITASITWVHNPEYKTRSNGTSALAAENAVDG
                     PFVLLMADHIFEVATLRRLLRTPIGPDEVILAIDRKIDRVFDLDDATKVVDDNGKLRA
                     IGKQLPDFNAIDTGMFLCTPALFDALRAVQDESDGSLSDAIRRIAAENRMRTFDIGDA
                     LWQDVDTPEMRREAERRLGDALRKPTDGPVSRLINRRISIPLSLFLTRTRVTPNQISV
                     FNLLLAACGGLLFASTSYPILALGGILLQVGSILDGCDGEVARLKFQQSKNGQWMDTV
                     TDNISYVALLVGLAIGQYRREPTSMTLWLGFVAIAAILTGLFIVYQRIHAMGKGSLLD
                     FKIPDRSRVDPTTARIFWIYEQLLPLIRRDIFAFGCCLLALANLPSVIFSLWVLGSVG
                     FMFGVIHITSPHVQRQLAPVPAVAKD"
     gene            12414..>12614
                     /locus_tag="P5179_04035"
     CDS             12414..>12614
                     /locus_tag="P5179_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94421.1"
                     /translation="MRVCLDARCLRKTVTGLGRYAEQLIRGLAAIDRTTDYVVILAKD
                     YECQPVEQANSQIVRFGGRLDTL"
BASE COUNT         2427 a         3738 c         3904 g         2535 t
ORIGIN      
        1 gccggcgacc gtatcctgcc gacgccatgg acacgcgctc ccttgctgat ccgcgtgagc
       61 gcccctcacc gcgagtccgc gcagatcgtt atcgggtact ccgcccgcca tgtcgaccga
      121 ctcatcgaag acacggttcg cccggcggtc ctggatgaga agtccggcgc atggcgtatc
      181 ctcacatccg ccagcgtcaa catccgtgcc cgcaccatca ccagccagtt cgtacaaagc
      241 ggcctctgga ctatctgcgg tctgagccgc gtccggtggc aggcaccgca gcgcgagtca
      301 aacgtcaccg tcggggacat cgacggtgac ggcaggctcg aggtcgcact cacacaatat
      361 gcaaacgccg aactcctgaa ctccgacggc tcgtcgcggc acgtgttcca gctccaggca
      421 agcaatcgcc ctgttcaaaa cacatccagc cctctgattg ccgacatcaa cgccgatggc
      481 agaaacgaga tagtgtacgg cgcaaccagt ggccacgttc acgccttttc gcctgacggc
      541 gtggagttgt ggcgtacgcc cgtcggcggc gaggtgcgcg gaggcattgc cgccgcaatg
      601 tccgatgatg gccgcccctc gtgcctgtgt gtctcatggc atgacgccgg tgtcgcgctg
      661 ctcaaccccg atggctcagt gcgctggcaa cattccctta cgcctcccgc agataccacc
      721 ccggtcgtgt gtatcccccg cgcaggcggg cccttcgtgg tcgtggcgca cggtgaggga
      781 ctcatcgcgt tccgttgcgc cgacggtgag gtgctctggc agtaccggac ggagggatac
      841 tctcccgtgg caccggctgt cggggagttc tcaagaagcg aggagccgca catcgtgacg
      901 ggaaccgcgt cgggaatgat ctatgttctg gatgctcacg gagaagtgcg ttcccgctgg
      961 gttgtgcctg ccgtgcacga ggcgtatcgg cccataatcg aggtcggcat ggcttctctg
     1021 cgcgattcgt ccacccgcca gatcgtcgcc accacggcgg gaggtgtcgt cgttgcgtac
     1081 gaacgcgatg ggacaccgct ctggcagttc gtgagcaggg aacaggtagc cgggttcgcc
     1141 cttggtgtgg gagggaggct cgcgtttgct gacgtcaatg ccgacggaaa cgacgaggtc
     1201 cttgtcgccg aacaggacca gtacgtgtac tccctcaagg gcgatggctc cctcctgtgg
     1261 gagttccggg gagcgttttt ctaccactat tccccggtcg tagcagatct ggacgggcaa
     1321 ggcgacttga cgctcctcac cacatcgccc tgtcagaacg gtacatacgc gcttcgagca
     1381 ggacaccgtg tcgccggtgc ttcgcggact ccctggccga cgatgcgggg gaattttgcg
     1441 cgcaccaatt gcgcaccgtg gccgcgctga tgaggttgct tctccgagcc tcgcggatcg
     1501 cgcagagcag cagtcttctc tggaaaactg acgcggtcag gcctcgcgct cgctcatccc
     1561 tgattccttg cgccaccggg caaaaaactc gttcgcatcg gggtgatttc gaagcgcgac
     1621 gtcaatcgcc gcgatgtatt tgcgcaggat agccttctct ctgcctaccc gctggtccac
     1681 ctgcccgaca agacgacgat gcagctctgc catttcggcg aactgacgat acgacatccg
     1741 cgcgtcgaga gaccgcccgc ttcttccgcg aaatccgaaa cgttccactt gaccgtccac
     1801 ccgctttgtc taccttcaac attgtctgat tcaacaccgc ttttcgctcg tacgttcagt
     1861 cttccccgta actgcctgag aggaccggca attccatgca ttacttcgca tacgccaatg
     1921 aagttctgcg cggtgaggaa gtcgacctgc gggatatcgc agaatccgtc ggcacaccgt
     1981 gctatgtgta cagccaccga acccttcttc agcacgcgca tgttctggat gccgcatggg
     2041 gagaacgtga acacctcacc tgctactccg tcaaggcatg ttccaacatc gccattttgc
     2101 gcctttttgc gcaaatgggg ctgggtgcgg atatcgtttc aggtggcgag ctttaccgcg
     2161 ccttactggc ggggtttcct ccggacaaaa tcgtctacag cggggtcggg aaaacccccg
     2221 aggaaatgcg cgaggcgctc cgcgccggca ttttctgctt caatgtggag tcggaggccg
     2281 aactcgtcga gctgaatcgt gtcgcgcagc gagagtaccg tcgcgcaaga atctcgttgc
     2341 gcatcaatcc cgaaatcgat cccaaaacgc atcccaaaac tgcaaccggg ctgaaaacgg
     2401 cgaaattcgg cgtgcctcac agcagggcgg tcgaaacata ccggcgggcg tccgaacttg
     2461 agtggctcga agttatcggg attgatgcac acatcggatc gccgatcatg agcgtgcagc
     2521 cgttcgtgga cgccgcgaag cgtctcgtgg acctcgttgg tcatatccgt gctgaaggca
     2581 tcgagctcaa atggatcgac atcggaggcg ggctcggcat cacctacgat gtcgaggatc
     2641 ctccaactcc caaagaatgg gcaactgcgg ttggcgacgt tctgaacgac gcgggcctcg
     2701 gcatcatcac ggaaccggga aggtccatcg tcggaaacgc tgcagtgctt cttacgaagg
     2761 tcctgtatct gaagcgaaac gaggacaaga atttcgtggt ggtagacgcc gcaatgaacg
     2821 accttatccg cccgagtttc tacgattcat tccaccgcat ccaaccggtg gtacaacgtg
     2881 gaactgagcc tttcgtggca gacgtggtcg gcccggtgtg cgaatccgga gacttcctgg
     2941 ccaggaatcg ctcgatcgcg cgaccggagc agggggatat cctggcggtc atgagtgccg
     3001 gcgcgtacgg gtttgcaatg tcatcgcagt acaactcccg ccggcgcgcc gcggaagtca
     3061 tggtgaaagg cggtacctgg caggtgatcc gcgacaggga gacttgcgag gacctagttc
     3121 gtggcgaacg ctttttcacc ctctgaaact agaggcgata ccgtgaacgc cgacggcatg
     3181 acggaagcgg acctgctcaa aacgctgttc ggtacccttc ctccctgcgg cgcggacgtt
     3241 atcgtgggcg ttggcgacga ttgcgcagtg cttcgcgctg acggcgatga cgtgcaggtt
     3301 ctcaccacgg acgccctcat cgaaggggtg gattttgtcg tcgggcccgg gttttcgttc
     3361 gaaaaccttg gacacaaagc gctcgccgtc aatctcagtg acattgcggc gatgggcgct
     3421 atcccgcggc atgcacttgt cacgctgtgc atacccgaac actacggccg caaagagatg
     3481 gcggaactct accaggggtt ttctcgtctc gcgagccgcc acggcgtcgg agtgatcgga
     3541 ggcgacctgt cgtcctcaag gaacgacttt atggtcagcg tcacactcct cggcactgcg
     3601 tcccgttcgg agatcctcct gcgaagtggc gcgaagcccg gcgatgcggc gatgcttttt
     3661 ggcaggatag gggactccgg cgcaggactg gacatcacgc gtggagcaca ctgcccgtct
     3721 gaggcgattc tgctcgaacg gcattttcgc cctgaaccgc tggttgacca ggggagatgg
     3781 cttgcgaatc gtacaggcaa ccacgtccac gccatgattg acatatccga tggccttgct
     3841 tcggacgcgc gtcacatctg cgaggcgagc ggggtgggtg tgcgtattga tcacgcctcc
     3901 ctgccggttt ccgacgaact cgcggaattt gcccgctaca cgggccggga acttgacggc
     3961 tatcttctcg gcgcgggtga ggattacgca cttttcgcgg cagtggatgc atcggctgcc
     4021 actgagatcg ccggccgttt ccgcagcgca tttccccgga caccggtcgc caccgtcgga
     4081 tcattccgcc gggggaacca cgtcgaaatc cgctgggaga atggtgccgt ttccgaactg
     4141 gcgggaggtt ttgatcattt cgcgcggcaa taaaggaggt ctcaatgcct gaccagaaaa
     4201 tcgacgtggt acggatgagc ttcgaggaag aacgcgcgtc cgcttatttg taccgagcga
     4261 tggaatccgt cgagacaggg gccgcggcgg cgatgtttgg caaactccgc aaggcttccg
     4321 aggcccagtc ggaaatatgg gcggaacagc tcagttcact gggcgccacc gtgcccgcgt
     4381 tccggccttc tctccgcgtc cgggtcgtgc gatggttgct gttccggctc gggccacgcc
     4441 gtatgcttcc cgtgctcgcg gggatgaaag tccgcgggct ttcgatttac agcaccagag
     4501 gtgcaactcc ctcgacggnn nnnnnnnncg aggaggcgct cgcctccccc tccaccgcgg
     4561 gcgagaaatg gcatcgcgcc gcacagggag gtggcgcctt gcgcgcagcg gtgttcggca
     4621 tgaacgacgg gctcgtttcc aatgccagcc tcatcatcgg agtggcaggg ggagcggcgg
     4681 atcagcccga agtggtcctg cttgccggag tcgccggtct gattgccgga ggtttttcga
     4741 tggccgcggg ggagtatatc tccgttgcca cgcagaagga gctctacgaa caccagatcg
     4801 cactggaaaa agcggagatg gcggtcttcc ccgaggagga aatcgaagaa ctcgccatgg
     4861 tctacgaaac gaaggggttg ccccgggatc aggcggtcgc cgtcgcgaca agaatcgtga
     4921 acgatcccgt caacgggctt gatactctgg ccagagagga actcgggctc gatcccgcgg
     4981 gattgtcatc accggttgca gccgcctccg cctctttcgc cgccttcgcg gtgggcgctc
     5041 tcattccgct gatgccgtat ctcgtaaccc gggcacccgc gacgctctcc ctcactgtcg
     5101 tggtaaccga ggtcgcgctt ctcctggttg gggccgttat gagtctcttc accgggcgga
     5161 acatgctctg gagcgccctc aggatggcac tgatcgggtc ggcggcggca gccattacgt
     5221 tcgcgatcgg gcgcttgctt ggcgtcagta tttcgtagcg tcgaatacat cgctggtttc
     5281 ggagagagaa aggcattgca tggaatggga gatggtcgtc aacccggaaa cgattgtcgg
     5341 tgaggctcac gcgtcgacac tcatcgaaag ccctgggggt gatctcctct gcgcgtggtt
     5401 cggcggcgca ccggaaggat cgcccggcac tgcaatctgg atgtctcgac ggacgagttc
     5461 ggggtggtcg ccggcttccg taatcgccga tgaaccgggc gtgccgtgtt tcaaccccgt
     5521 cttcttccga cacggaacaa cgatctgggt gtacttcaag gccggcaagg aagttgaaaa
     5581 gtggagcggt tttcggacca gatccgagga tggcgggttc acctggtccg ctccggagat
     5641 tctgccggcc ggcctgttcg gcccgatcaa gaacaaaggc atcaggcttc acaacggcat
     5701 gatcgcattc ccgagttccg cagaaagcta tcgtgcatgg acggggtggg tgtaccttca
     5761 cgatcccgat tcggatgcgt ggaaaatacg cggccccctc gcccatccgg acaatccctg
     5821 gggagtcatc cagccgacgc tctgggaggt gacgccaggc cacatacgcg ccctgctccg
     5881 atcccgaaag gaaattggcc gcgtgtgcgt atcggattca tttgacagcg gcgaaacgtg
     5941 gacgcctgcg cggccttcat ctctcccgaa tccaaacagc ggaatcgacg ccgtcaaact
     6001 ggctgacggg agagttgttc ttgcctacaa ccacactgac ccctcaccgc gtctgtcggc
     6061 ggcaaccggt gacatgcctg gaaacacccc gcttctcggt ggcagacacg aaattcacct
     6121 cgcagtatcc gaagacggag gggaaacgtg gtctccaccg tgggaaatgg aaatcggcgg
     6181 tgcggagttc tcgtaccccg cgataataca gtcaagcgac gggcttgtgc acgtcacgta
     6241 cacctggaac cgcaaaggaa tccggtatgg cgcgtttaca ccctcggaaa tcagcctgat
     6301 ctgttccggc acctttccgc ggccagcacc gtaaagagca gcctatgtgc ggtatgaccg
     6361 ggcacgaaca tcgcggcgtg cgagaaaact cccgccggcg gctggacccc gttgaggaga
     6421 aaatccttct tctccttgct ggtcgcgccg aagacatggt ccctctcgtc gatatcacga
     6481 ctgatctggc gggcctgccg gatcatctca ggggacaatg gttcgacaag aaattgctga
     6541 cgccctccca ggtcgccgcc tgggaggaag ccctgaagca ggtgaggcgt gcggtgcggt
     6601 ccctgttccg caaaggcttc atcgacgtgg gcagagaaga tgagttcccc gagggcttct
     6661 gccggcacca cgccgaggtc atccgggcgc tgcaaaacca tccggcttgg aagggcgata
     6721 cagacacctc gaaacgctct gtcgcgggga ttgaaagcga aatccgcgcc cgcatgccct
     6781 gggcggacgc acgggatctc accgccgtcc agaagcagat ggacggcccc gtagacattg
     6841 tcggcatcac ttcatccggc cgtgcggaac tcacctccaa ccccggtcaa tcccttgctg
     6901 gttctcagcc tttctgacat cagcgtgccg ttcggcgaat cggaagacgc cttgcgtgcc
     6961 ctctgcgccg ccagactcgg tgttccagag gccgaactcg ggcagttgcg ggtacgtcgc
     7021 cgtgctctcg acgcccggca cccctcacgc ctgcggtatg tgtacaccat cgaagtcgag
     7081 gccccgttga cactggagca ggcccgacgg atttctggcg cgaaagtcgt ggattcgacg
     7141 ctcgcacgac gcgtcgaatt cggcacccgt gcacttttgg agaggcccgt ggtcgtcggc
     7201 gcggggccgg cgggtttgtt cgcagcctgg aaactcgcag agaacgggta ccgaccaatt
     7261 ctgatcgagc gcggcaaacc tgtggaggct cgcaggagag atgcgaccga cttctggaaa
     7321 tatggaagac tcgaccccga ttcgaacgtc ttgttcggtg agggcggcgc cggagcattc
     7381 tccgacggga agctgacgtc aagatcgaag gattggcggc gcgatgacgt tcttgatcgc
     7441 ctcgcacgct gcggagcagg tgatagcgtt cgttttgaag cccggccgca tgttggaacc
     7501 gacaggctct tctacgtgct gaaggagatg cgtgccacat tgagcgcgct gggcacggag
     7561 atcctgtttg aaacccgggt ggaggatttt ctcattgacc ggggaacgct tctgggcgtg
     7621 cgttgtgcgc cggacgtagt catccccacg aacgcgctga tcctggcatg tgggcacagt
     7681 gcccgcgata cctaccatgc gcttcgggca cgcggtgttg cccttgaaca gaagccgttc
     7741 gcaatgggga ttcgcgtcca acaccggcag gagattattg acagggcgca gtacggccga
     7801 cgggccgttt cccacgaagg aagggaccga tcccttcagc ctgccgaata cgtactccga
     7861 tgcccgaaga caagcacagg gagatcggtg ttctcctttt gtgtgtgccc cggcggcgcg
     7921 gtcatcccgt gtgcgtccga acccggtggg ttgttctgca acggtatgag cggcaggaac
     7981 cgcggcggca ggttcgccaa tggcgcgatt gtcgcacaga ttccgaccgc tgacttcgcg
     8041 tcagcggatc ccctttctgg catcgacttc cagcgacact gggaaagggc ggcattcaaa
     8101 tccaccggag gaacgtatgc ccttccaatg atgccgctgg tctctttcgt cgacggttca
     8161 tggcctcccc ggcgtgtaca cggattggcg aaaggtcttt tcccgcgcgc ggatctcgtg
     8221 gacctcacgt ccctgcttcc tccctctgtc gttcaggcgg tcagagaagc gtcgcatgag
     8281 ttttcgaaaa ctatccacgg ctggctggcc cccgacgccg tcgcgtttgg ggtggaaaca
     8341 cgaacaagct cccccgttcg catccttcgc agagaggatg ggcagtcaga aaacgtcgcc
     8401 gggttgttcc cggcagggga aggcgccggg tatgccgggg gcatcgtcag cgccgccgtg
     8461 gatggaatgc gcgcggcgga gcatctcatg gcgaccttcg cccctccctc ctgatctttt
     8521 cgcgcgtccg cctagcctac ccttcttgca ccggaacgca atttgcacag tatccggtcc
     8581 ggaagcgact gatacgcggc acgcccaccg aaggaatttg gcctgccgtg tgcgcccttt
     8641 ctccgcatct ggtcgcgcag aaggctagat gaacaaagtt tcatcactgc ttcagttcct
     8701 cttcatattg ccgggataga ttttctgggg taagaaggag aactcgcagg tgatttcgga
     8761 aatacgggca caactgcgga tttcgcggcc tcccgaagcg gggttcgccg tccgcggctt
     8821 ttccagcgaa aggggagtcc ccgtgcgcgt actcctcgtg gaagacgatt cccgtatcgc
     8881 ccatttcatt caaaagggcc tgacggaaag cgggtacgtg gtagatctgg tggccgacgg
     8941 cgtcgaagca gtgtcgcagg tcgcctccac cgcgtatgat gtgatcgttt tggatgtcat
     9001 gctgccccga atggacggat tcggcgtact ggaatccctg aggcagcaag ggcagaagat
     9061 gccggttctc atgctgaccg cgcgcgacac aaccgacgat aaggttcgcg ggctggatgc
     9121 aggggcagac gactatctga cgaaaccgtt ctctttcgct gaactggaag cacggctccg
     9181 ggcgcttctg cgaaggggaa ccggcgaggc aagcgcgcat ctgcgcctgg gcgatatcac
     9241 ggtggacccc gtcagccacg tcgtgaagcg cggcgataca gtgatagaaa tgacaccaaa
     9301 ggaatacgca gttctggagt atttcatgcg gtacccgggc cgagttctga cacggaccat
     9361 gataatcgag cacgtgtggc aatatcagtt cgataccgac acgaacctcg ttgatgtata
     9421 catcaacagg ctgcggcgga aattgaacga cgcggaaggg cgcatactcc agacaatccg
     9481 cggggtcggg tacacgttga ggccggcaca gtgaaaagcc gaagtctgcg attcgagttg
     9541 acgttctggt attcgacggt tatggcagta accctgctcg tcatgggtgt cgccgtcgaa
     9601 caggtggcgt acaaccggat ttcggccagc atagataact cgttgcggca ggctgcattt
     9661 actgtaatca gtgaagtggg tgtgcttcgt tcgaaacccg atgccgaacc tttcggggag
     9721 accagtacgg tgctgaatcc gccgccgtgg ccgccgcgct acgtgcagat tctttcggca
     9781 tccggagagg ttctctaccg ttcggccaat ctggggcagt atccgcttcc gatagactcc
     9841 gctgcgttgc gagaccgtgg tcgtggcatg gtgatttctc ccgacatgcg tctgcacggg
     9901 gaacccatcc gcatggtcac gtttccgtta ccggagtaca atggaagcaa cgccgcgtgg
     9961 ggacaggttg cgctttcgct ccacgatctg gaccgcgcga gaaaaaagaa ccaactggcg
    10021 cttgcgatta tcctgcctgc cgcgattggc atttcggctg tggcgggttg gtggctcgcc
    10081 agacgcgcac ttcggcctat cgacggcgtg atcaggaccg cccagcggat acgtgccgcg
    10141 aacctgaatg agcgaatcgc gccgagggac gtagatgacg agctgggacg gctgatcagc
    10201 acgctgaatg aactgctgga gcgtctcgag ctgaacttcc ggcaggtaag cagattcagc
    10261 gccgacgtat cgcacgaact gcggacgcct ttgacgatca ttcagggcga ggctgaggtt
    10321 gcattgcgcg agggcgccag cagggaagag atgcaactgg cgcttgaggt ggcgctggac
    10381 gaggcacagc gcatgtcgaa gctggtgaag aatctcctga tgcttgcgcg ccttgaaacg
    10441 ggtcaggagc gacctcactt cgtggagacg ccccttgcgc cgattatcga ggacgtgagc
    10501 gaagaaggac ttgctctcgc ccgcgcgaaa ggcgtcacgt tttcggcaca taccgttgaa
    10561 aacgcctccg tctggggtga tgccgttttg ctgcatcaac tggcgttcaa ccttgtggac
    10621 aatgcggtca aatacacacc cgcaggcggg acggtggaac tcagcctctc cagaaacggg
    10681 tcaagcgtac gtctttccgt gaaggacacc ggcgttggaa tcgacgaagc cgaactgggt
    10741 agaatcttcg accgttttta ccgtggggac agcgcacgca accatactga gggcggaagc
    10801 ggcctcgggt tggcgctcgt ggcacagata gcggaagcgc acaaaggcac cattcaggtt
    10861 accagcaaac cgggcgaggg cagcgtcttt accgtgacgt tcccgctcgc gtcacaagct
    10921 ggaaaagcgc gaccggatat ggcagaagtc tcgatgattg aagggattcg ttcatgattg
    10981 aaacagccgt gatactcgcg gcaggtaacg gaagtcgtct tcggggtgtc tccgcgggcc
    11041 tcccgaaacc gctggttccg ctggccggtg tgccgatcat cgtcagggta atgcgcgctg
    11101 cacaggaggc cggtatacgg aaattcgtgg ttgtgacggg ttaccaggcg gaaaaactcc
    11161 gggctgccat tgagaatcag cccgccatta cggcgagcat cacgtgggtc cataaccccg
    11221 agtacaagac gcgctccaac ggcacgtctg cgcttgccgc cgaaaacgcc gtggacgggc
    11281 cgttcgtgtt gctcatggcc gaccacattt tcgaagtggc cacgcttcgc cgtctgttga
    11341 ggacgccaat cggcccggac gaggtgattc tggccattga ccgcaagatt gaccgtgtct
    11401 ttgacctcga tgatgcgacg aaggtggtag acgacaacgg gaagctgcgc gccatcggca
    11461 aacagcttcc agacttcaat gccatcgaca ccgggatgtt tctgtgcact cccgcgctct
    11521 ttgacgctct tcgcgccgtg caggacgaga gtgacggcag tctttccgac gcgatacgtc
    11581 gcattgccgc agaaaaccgg atgcgcacgt ttgatatcgg cgatgcgttg tggcaggatg
    11641 tggacacccc cgaaatgcgg cgggaagcag agcgccgcct gggagatgcc ctgcggaaac
    11701 cgacagacgg ccccgtgagc agattgatca acaggaggat ctccattccg ttgagtctgt
    11761 tccttacgag gacgcgggtt actccaaacc agatctccgt gttcaacctt ctgcttgccg
    11821 cgtgtggcgg tttgctgttc gccagcacgt cgtacccgat tctggccctc ggaggaattc
    11881 tcctgcaggt cggttcgatc ctcgacgggt gcgatggcga ggtagcgcgg ttgaaattcc
    11941 agcagtcgaa aaacggtcag tggatggaca cggttacgga caacatttcg tatgttgctc
    12001 tcctcgtggg gcttgcgatc ggtcagtacc gtcgggagcc tacgtccatg accctgtggc
    12061 tcggttttgt ggccatcgcc gccattctga ctgggctctt catcgtttac cagcgaatcc
    12121 acgcgatggg caaaggcagt cttcttgatt tcaagattcc tgaccgtagc cgtgtggacc
    12181 cgacgacagc gcggatattc tggatttacg agcaactgct tcctcttatc cgccgcgaca
    12241 tttttgcatt cggctgctgt cttctggcgc tggcgaatct gccttccgtg attttttcgc
    12301 tgtgggttct ggggtcggta ggcttcatgt tcggggttat tcacatcacc agcccacacg
    12361 ttcagcgtca gcttgcgcct gtcccggcag ttgcaaagga ctgaaaggtt tgcatgcgcg
    12421 tctgcctcga tgcccgttgt cttcgcaaaa ccgtgacagg cctggggcgt tacgcggagc
    12481 aactgattcg tggtctcgcc gcaatagatc gcaccacgga ttacgtggtc atactggcaa
    12541 aggactacga gtgtcagccg gtcgaacagg cgaattccca gatagtgcgc ttcggcgggc
    12601 gcctggacac cctc
//
LOCUS       DAOTNG010000054        12556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4812_length_12556_cov_5.709143, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000054 DAOTNG010000000
VERSION     DAOTNG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4812_length_12556_cov_5.709143"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="P5179_04040"
     CDS             complement(<1..1565)
                     /locus_tag="P5179_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS94422.1"
                     /translation="MIRWSSSKARWVAALACALATLYGTSLRAGTTGKIAGQVTDRAT
                     GQPIPGAAVLIEGLRLGSTTDADGRYFIIGVPPGSYSVKASLLGYAPITREGVIVNID
                     RTTPADFQLVSSAIEVEALTVVAPREVIQLDVAGSRSTLAPEDVVALPNVTSLAGAIS
                     AEPGGNFRSGLAEETQLMLNGHMMTDDRVARYNASSIPITSVQEVQILTSGFNAEYGN
                     VRSGVINVVTRDDAQRIWMNGRATYTPAQKKHWNGSAYGKSTKEWQVYGSDASITTPY
                     KIDNIQTTAPGDSITVFPGWIKWIPTLPASRRTHADSVARADSMLQLWRHHHRIMETD
                     YANAPDYMFDVSVGGPIPFVDGLSVVYSHRNTRTLYALAAARPAFKDFTEQMTLTYRL
                     GRSAKISFTGKYTEESGLGDVITGTSSDGTVGGNMMYGGWQKTAVSKYAMYANAPMDN
                     KFWLFGANFTHVLTMNTQYELGFDYQYNDYLTGQWPMRYLAPIAGRAVVNPIWNDQRL
                     SPGDSVGVWKIYTS"
     gene            complement(1919..3850)
                     /locus_tag="P5179_04045"
     CDS             complement(1919..3850)
                     /locus_tag="P5179_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94423.1"
                     /translation="MNSEPNSVARFTGRAVTAGAVISLLMAIGAPFSRYVLYSSPLVF
                     TSFSWGVVTTWLLFVVFEMGVLRRFRFWRPMSSPEFAVAFMIATVGSSLTTTEVAGPI
                     VTHIGGIHYFASPENRWHETLDSLLPVWLLPTDTHNAVKWLFDGVPQGQTYPWADWVV
                     PLFWHGSFTAAFLLVQFALVGLLRKHWVEHERLTFPIMQMAVELIQQPEEGEFRPPWS
                     RPRLFWFGFFIPLTFLTLQMLSWFWPTVPQLPTNLGAISFGTEYPALNMNLYWPVIGI
                     AFFANKEVIFSLLFFTVLGTLVTGWLTRLGIDVGVGAQPMQWLNMGALTVMVLWFLWL
                     ARRHLWSAARRAMGKPDGDDDSNEFISYRTAYLIIIGAVAYMVFWMQVSGSSIAIALL
                     LIVMGTIVYLGIARLSFEAGVFHVNAPLRLVDMIVDATGSANISHASMAALAQHYWRF
                     SNVKSLFLVTFGHAGALSQQMEIPRRRLNPAIVIVTVATTLLATWYTLYLGVAYGGYN
                     FTDWIFRSGPADPFISLMKWISNPLPADFRRITFFGIGGGLMVLFSVLRYAFPWWPLH
                     PIGLAICFSFHIKESILSFLIAWVAKSLTLRLGGIKLFRRTVPLFVGIVVGAFVGTAM
                     CFLVDMIWFPMSGHAVEFK"
     gene            complement(4039..4296)
                     /locus_tag="P5179_04050"
     CDS             complement(4039..4296)
                     /locus_tag="P5179_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRS94424.1"
                     /translation="MPYVICEPCIGVKDTACVDVCPVDCIHPTKAEDAFETSEMLFIN
                     PEECIECGACEPACPVAAIYPEEDVPEEWREYIDRNAEYYR"
     gene            4618..6279
                     /locus_tag="P5179_04055"
     CDS             4618..6279
                     /locus_tag="P5179_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94425.1"
                     /translation="MVQSNPAESQPFALRGWGVRVWNNYDLFDAEVRKAAREAGRYHI
                     NLLDLHDGTVPPGIGWVELFAQYRHVQALRDKSSLTYKGTVIPERDRETYRQKFRELC
                     TFVKSQGLRIHVWYHVLRDLPEEWLIAEPTISRLEGRRLWQVIGSMLEDLFTGVPEID
                     ALVVTAGGTTAYPTRDQSSVTPGERLRAVYQCVYEACRRHRKQFIIRETGDTPSESDT
                     FLYAIGPLPPEIQIMVKNVQKDWCHLTAPVNPTLQRLGGKTVLLETDLYGEHWGRSAL
                     PLARPGYVFELVRSWLPLKAAGAVGRIMVQEEEDGVETSIFSSPGTVNLHAFGKVLAA
                     PGTTFQQAGTWLPEFDELDRGLWYEWAAQFGNKNGNVSPCLVSALETSPLICLYTLYL
                     AGAYFQDRSFFPGIETFEKSIWPTFVRKSKHIDLDVLLWEKDEAVRIAERSLEDLQRA
                     APTMDPQAVEVFTQFFEHAKDIAAAFRSLVELCAGRVKPETLRTTCRDANDLATRIEI
                     LRGPGFYGRLPERLRELSAYVQDHTTERSERADDAPPAEQEPTGP"
     gene            complement(6293..6946)
                     /locus_tag="P5179_04060"
     CDS             complement(6293..6946)
                     /locus_tag="P5179_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS94426.1"
                     /translation="METLSVSELFVSIQGESLAAGRLCAFVRLAGCNLRCAYCDTNYA
                     WDADAGRAMAVDDVAAWVSGSGVPLVEITGGEPLLQPAVYPLMETLIGRGLEVLLETN
                     GHVDARGVPERVTRIIDVKTPGSGMAEGDYLVNLPSLRENDQIKFVLTDRSDYDWAKE
                     VLAAHPELGRVSAILFSPAFETLPPRDLAGWIIADRLPVRFQLQLHKYVWGPQERGV"
     gene            complement(6995..8530)
                     /locus_tag="P5179_04065"
     CDS             complement(6995..8530)
                     /locus_tag="P5179_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS94427.1"
                     /translation="MKDRAGKSTDLARPNVLLIMTDQQRADSLGCAGHPAVRTPHIDG
                     LAASGVRFDCAYTTHPTCTPSRGAIFTGRYPSSHGARMTGMILPESERMLPAVLREHG
                     YETALFGKPHFAPMGDYADCPSREMRHLWNDGAHPIEGDYYGFENVVFFGGHGPGIWG
                     HYRDWLRERDPAAERVIENASGDGGYMRAGAYEDGLADMFRSPLPAELHPTSALGEMV
                     SDYLRRRHEKPFFLVASFSDPHHPFAPPAEYADRYDPGDMPVPVPPDSDGFGTVPPHF
                     GRAYRGQSGMFSGGGHVDYSAIPPAAMLRARALTCGMVELIDAAVGNILRTLDETGLR
                     RSTVIVFCSDHGDFLGDHGFLYKGPMHWDGLTRVPFIWNLPGALHSGVVESGVASTLD
                     VTPTVLDLCGIPPEPGIEGISLARYLRGQETYARDEALTENDDDYLGERLRTLVTAEW
                     KITAYAGRPWGELYDRVNDPHETRNLWDDPAFRGRRDEMRLRLLDHLMRREDRLTVQR
                     WYA"
     gene            8680..>9257
                     /locus_tag="P5179_04070"
     CDS             8680..>9257
                     /locus_tag="P5179_04070"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRS94428.1"
                     /translation="MHPLLLQLDLDHPGLEEANALARASRIHEALAAVVRYFRARPEP
                     DPSLLATPNPGTVEASERAIAREFVFYQVPGKVPPGNWDWTWRPGTDWEWTYALNRHH
                     WWAELAAAYLATGDQRYAQELDSLIRSWVGQCPVHVDDISSWRTLETGIRVAGPWIGI
                     LSAMKATPVITDTAWIMYLRAIHDHAEHLVAHP"
     assembly_gap    9258..9357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9358..10104
                     /locus_tag="P5179_04075"
     CDS             <9358..10104
                     /locus_tag="P5179_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRS94429.1"
                     /translation="DALTFEIMAYGQPLIGTMGRYSYDPVPVRRYLVSSAAYNTVMID
                     GQGQKWTKTHPDRSTWVAREPTADPWSSTAEQDVAYGRFNGPWTGDLSGVVWERRMAF
                     HKPDAKLRRPGFWVVRDTFTGEGEHELRFLLHFYPGNVVCDDETHTVRSDYSGAQANV
                     IVAFEHPEAVKFDCAYGQEDPPRGWYSARYGVIEPAWEVAVVRAAVFPADFVMVFVPY
                     QAEAKLPVRVKREQGVVSVQLDDREWLVSV"
     gene            10150..11763
                     /locus_tag="P5179_04080"
     CDS             10150..11763
                     /locus_tag="P5179_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94430.1"
                     /translation="MKLHNAFLVNGQPILGTYVASGVEELALCKEIGMNVVIGGHTEL
                     DVTTPEGAFCQQNGIKVMHHLVQHIYGKPRLADFVTPAQTEIPFVADLMKGVAQPSDE
                     LPKGVPSSGVFQIDDETVYYGKLTATHFTECVRGYRGTKPAEHRAGAFLFFPEACAEE
                     IRKVRTSPNLWGYYVLDDSPGDALSALRALYRTVKEEDPGRPVCAGYGSAGSLCNFGP
                     DVCDVMMFYWYPVAESWEPVAHGQYFRMLTPHQVQYMLMAAREMVPGIPFVGVYQSFD
                     AKFDPDQKNIAVPTADQIREQIEDYVREGACGLIAFLCSKKHFGFAHYPYMVEELKAI
                     HHEILETGELQVRPETEEMKRTRVQPVGFWEKPLPLPGVIPAWWTAFPFEDAKGEMLE
                     ATFGPDHEFDLDAVYPGKTWPVRWTVRRTFGGSLGFGELLGPHSYTKNCVAYAVCEVE
                     SPKQQEVILSIGSDEDALIKLNGKEVWRFGGGRGLTRDSDRVRVTLPQGKSRIFFKVR
                     NRQGLWGLDLRFLDGEGKPLEGLKFSPQP"
     gene            11849..12439
                     /gene="purN"
                     /locus_tag="P5179_04085"
     CDS             11849..12439
                     /gene="purN"
                     /locus_tag="P5179_04085"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRS94431.1"
                     /translation="MNLGVLASGGGTNLQALIDRSEAGTLAAKVVVVISNNSTAGALE
                     RARRHGIPCYHLSRATCGDEASLDAAMCETMRRHGVDLVILAGYMRPVGPGLLQAYPD
                     RVLNIHPALLPKFGGKGMYGIHVHEAVLAAGDKTTGVTVHLVNSEYDRGRILAQRTAP
                     VLPGDTPESLQKRVLAIEHEIYADVINRIAAGEIRL"
BASE COUNT         2661 a         3811 c         3618 g         2366 t
ORIGIN      
        1 gaggtgtaaa tcttccagac gccaacggaa tcccccggag aaagccgctg atcgttccag
       61 atcgggttca ccaccgccct gccggctatc ggcgcaagat aacgcatcgg ccactggccg
      121 gtgagataat cgttgtactg gtaatcaaac cccagttcgt actgcgtgtt catggtgagc
      181 acgtgcgtga aattggcccc gaaaagccag aatttgttgt ccatcggggc gttggcgtac
      241 atcgcgtatt ttgatacggc ggttttctgc cagcccccgt acatcatgtt tccgccaacc
      301 gttccgtctg agctggtacc cgtgatcacg tctccaagcc cggattcctc cgtgtatttc
      361 cccgtgaagc tgatcttggc ggaccgcccg agacggtacg tcagcgtcat ctgttcggtg
      421 aaatctttga acgcggggcg tgccgccgcc agagcgtaca gtgtccgcgt gttccggtgg
      481 gagtacacta cgctgaggcc atccacgaac ggtattggac ctccaaccga cacgtcgaac
      541 atgtaatccg gcgcgttcgc gtagtccgtt tccatgatgc ggtgatggtg ccgccagagc
      601 tggagcatgc tgtctgcgcg cgccacactg tccgcgtgcg tccgccggga tgccggcagg
      661 gttggtatcc actttatcca gccggggaac accgtgatgg agtcaccggg cgccgtggtc
      721 tgaatgttgt caattttgta aggcgtcgtt atcgaagcgt cggagccgta cacctgccac
      781 tcttttgtgg atttgccgta ggcggacccg ttccagtgct tcttctgcgc aggcgtatac
      841 gtagcacggc cattcatcca gatgcgctgc gcatcatctc tcgtcacaac gttgataaca
      901 cccgagcgca cgttgccata ctcggcgttg aagcccgacg tcaggatctg cacttcctgc
      961 accgaggtaa tcggaatgct ggacgcattg taccgcgcga cacgatcatc ggtcatcatg
     1021 tggccgttca gcatgagctg cgtttcctcg gcgaggccgc ttcggaaatt gccgcccggt
     1081 tccgccgaaa tcgcccctgc gagcgacgtc acgttcggca acgcaaccac gtcttccggc
     1141 gcaagggtgg agcggctgcc cgcaacgtca agctgaatca cttcgcgcgg cgcaacaacg
     1201 gtcaacgctt ccacttcaat cgcggacgac acaagttgaa aatcggcggg cgtggtacga
     1261 tcaatattga caatcactcc ctcccgcgta atgggagcgt aacccaggag tgacgccttc
     1321 acggaatacg agcccggcgg aacgccgatg atgaagtacc ggccatcggc atctgttgtc
     1381 gaaccgaggc gcaacccctc aatcagcacc gcagctcccg gaatcggctg cccggtggca
     1441 cggtcggtca cctgacccgc gattttgcct gtcgtgccgg cccgaagcga ggtcccataa
     1501 agcgtcgcga gcgcgcacgc aagtgcggcg acccatcttg ccttggacga tgaccagcga
     1561 atcattgact ctgcctcctg tcgagaggtc aacaactagg ccaaacagag gctgccgcac
     1621 cagaggcgtg acaacccgtg cgccgttcga aaattcaaaa aagtcgtccg ggaaacttca
     1681 cgaagtacga gaggagcgga ctgcgtatcc cccgcatctc ccttcctcaa ggtgacacgg
     1741 gcgtgcgtca tgcagcgcgg acacgcagtt gcagtaccca ttccgaccgc cttccctacg
     1801 gtgctggatg gtgctctccc caagtaatat atcccgacct cccatttcca tcaagacggg
     1861 gggtcgttaa agattcaacg cggcacacat tcaatttgcc acgtaagaag tatctgacct
     1921 atttgaactc caccgcgtgc cctgacatcg ggaaccatat catgtcaacc agaaaacaca
     1981 ttgccgtccc gacaaacgcg cccaccacaa tgccgacaaa caggggtacc gtgcgacgga
     2041 agagcttgat tcctccaaga cgcagcgtaa gagatttcgc aacccatgcg atcaaaaatg
     2101 aaaggatgct ctccttgatg tggaacgaaa aacagatggc cagcccgatt ggatgcaggg
     2161 gccaccacgg aaacgcgtac cgcagaacgc tgaaaagcac catcaaaccg ccgccgatgc
     2221 cgaaaaacgt gatgcgccgg aaatctgccg gcaacgggtt gctgatccat ttcatgaggg
     2281 aaatgaaggg atctgccggt ccgcttctga aaatccagtc cgtgaagttg tacccgccgt
     2341 acgcaacgcc aaggtagagc gtgtaccacg tcgcaagcag tgtcgtggca accgtgacga
     2401 tgacgattgc gggattcagt ctgcgacgcg gaatctccat ctgctgagaa agtgctcccg
     2461 catggccaaa cgtgacgaga aacagcgact tcacgttcga aaaccgccag taatgctggg
     2521 cgagcgcggc catgcttgcg tgggatatgt tcgccgagcc ggtggcatcc acgatcatgt
     2581 ctacgagccg caacggggcg ttgacgtgga acacgccggc ttcgaacgac agccgggcaa
     2641 ttcccaagta gacgatcgta cccatgacga tgagcaagag agcgatggcg atgctcgaac
     2701 cactcacctg catccagaac accatgtagg ccacggcgcc gatgatgatg aggtaggccg
     2761 tccggtagga tatgaactcg ttggaatcgt cgtctccgtc cggcttgccc attgcgcggc
     2821 gcgcggcgct ccacagatgg cggcgcgcca gccagaggaa ccagagcacc atcaccgtca
     2881 gcgcacccat gttgagccac tgcatcggct gcgctcccac gcccacgtcg atcccgagac
     2941 gcgtcaacca ccccgtaacg agcgtgccca gcaccgtgaa aaacagcagg ctgaatatga
     3001 cttccttgtt cgcgaaaaac gcgataccga taaccggcca gtagagattc atgttcaacg
     3061 cagggtattc ggtcccgaac gagatggctc ccagattcgt cgggagctgt ggaaccgtcg
     3121 gccagaacca gctcagcatc tgcagggtca ggaacgtgag cgggatgaaa aacccgaacc
     3181 agaaaagccg tggcctgctc cacggcgggc ggaactcgcc ctcctcaggc tgctgtatca
     3241 gctccaccgc catctgcatg atggggaagg tgaggcgctc atgctccacc cagtgctttc
     3301 gtagaaggcc cacaagcgca aactgaacca gaagaaacgc ggctgtgaaa ctgccatgcc
     3361 agaacagcgg aacaacccag tccgcccacg gatacgtctg cccctgcgga acgccgtcaa
     3421 acagccattt gaccgcattg tgggtatcgg tcggcagcaa ccagaccgga aggagggaat
     3481 ccagcgtttc atgccaccgg ttctccggcg acgcgaaata gtggattcca ccgatgtgcg
     3541 ttacaatcgg gccggcaact tcagtggtgg tgagactcga acccacggtc gcaatcatga
     3601 atgcgaccgc gaactccggc gaactcatgg gtcgccagaa acgaaatctc ctcaggacac
     3661 ccatttcgaa gacgacgaac agcagccatg tcgtcaccac accccaggaa aaagacgtga
     3721 acacgagggg tgaggaatac agaacgtagc gcgaaaacgg cgctccgata gccatcagga
     3781 gggatatcac cgcaccggcc gtcactgccc gcccggtaaa acgggccacc gagttgggtt
     3841 ctgaattcat gagcaactcg ccagcgtaag ggagaatctc gggctcacca ggaacaggtc
     3901 acactcgttg cgcgctcggg ttctggtatg ccgccggtgc tggaattctg aaaaaccggt
     3961 ggattaaaat ttcaatgctt gcttcggcgc gtcaatgggg ttgaccaaaa cacacgcaag
     4021 aaaagtctca cgcgccgctc agcggtaata ttccgcgttt ctgtcaatgt actcgcgcca
     4081 ctcctccgga acatcctctt ccgggtagat cgcggccacc ggacaggccg gttcgcacgc
     4141 gccgcattct atgcactcct ccggattgat gaacagcatt tcactcgtct caaaagcgtc
     4201 ttctgctttt gtcgggtgaa tgcagtcaac ggggcatacg tcgacacacg ccgtgtcttt
     4261 tacgccgatg cacggctcgc agatgacgta cggcattgcc ggacctcctg atgaagcgtt
     4321 gcgggaaaaa acctgccccc cgggcagggg cgctgcaccc gcgcacagca cccacgggtg
     4381 gttcccggga aagacgatgc gaagtgtagc acgggaagac ggtgctcgtc aacacgcccg
     4441 ccgtgaatct cagaaaaagc cccgggaacg gctttgtccg gatgctgatg ggccgcaccg
     4501 tataccattg ggttcccgga aaccttacct ttcttgatat gcgttctccg gccgaaccac
     4561 cggccgcaac caaaaacctg cgttcgagca acagcaccat ttttcgggga cacattcatg
     4621 gttcaaagta accctgcgga gtcacaaccg tttgcactcc gcggttgggg tgttcgcgtc
     4681 tggaacaact acgatctttt cgacgcggaa gtaaggaagg ccgcgcggga agccggccgt
     4741 taccacataa atctgcttga tctccacgac ggcacagtgc ctccgggaat tggctgggtg
     4801 gagctttttg cccagtaccg tcacgtccag gcgctgcgcg acaagagctc ccttacgtac
     4861 aaaggcacgg taattcccga acgggatcgg gagacgtacc ggcagaagtt cagggaactc
     4921 tgcacgtttg tcaaatcaca gggcctgcgc attcatgtct ggtaccacgt cctgcgtgac
     4981 ctgcctgaag agtggctgat cgcggagccg acaatttccc gccttgaagg acgccggctc
     5041 tggcaggtca tcggcagcat gcttgaagac cttttcacgg gggtaccgga aatcgacgcc
     5101 ctcgtcgtca ctgcgggcgg cacaacggcc tatcccaccc gcgatcagag cagcgttacc
     5161 ccgggggagc gtttgcgcgc ggtatatcaa tgtgtgtatg aggcctgcag acgccacagg
     5221 aaacagttca ttatccgcga aacgggcgat actccctcgg aaagtgacac atttttgtac
     5281 gcaatcgggc cattgcctcc agagatccag atcatggtca aaaatgtgca gaaggactgg
     5341 tgtcatctga cggcaccggt aaatcctacc ctgcagcggc ttggcggcaa gaccgtcctt
     5401 ctggaaactg acctgtacgg cgaacactgg gggcgttcag cattgccgct ggcacgacct
     5461 ggctacgtct tcgagcttgt gcgctcatgg ttgccactga aggccgcagg agcagtcggc
     5521 cgcatcatgg ttcaggaaga agaagatggc gtcgaaacca gcatcttctc ctccccgggc
     5581 acggtaaacc tgcatgcgtt cggcaaagtg cttgccgcgc caggcacaac gtttcagcag
     5641 gccggaacat ggttacccga attcgacgaa ctggatcgcg gcctctggta cgagtgggca
     5701 gctcaattcg gcaacaaaaa cgggaacgtc agtccctgcc tcgtgtccgc actggaaacc
     5761 tcccccctca tatgcctgta caccctgtac ctcgccggcg cctatttcca ggaccgcagc
     5821 ttcttccccg gaatcgaaac gtttgagaaa tcgatctggc cgacgtttgt ccggaaatca
     5881 aagcacatag acctggacgt cctcctctgg gagaaagacg aggcagtgcg aatcgccgaa
     5941 cgatcgctgg aagacctcca gcgggccgcc cccacgatgg atccgcaggc ggtggaagtg
     6001 ttcacccagt tcttcgaaca tgccaaagac atcgccgcgg cgttccgttc gctcgtcgaa
     6061 ctctgcgcgg gacgcgtcaa accggaaaca ctgcggacca cctgccggga cgccaatgac
     6121 cttgcgacac ggatagaaat cctccgcggg ccaggtttct acgggcggtt gccggaacga
     6181 ttgcgcgagc tttccgcgta tgtccaggat cacacgacgg agcgcagcga acgcgctgac
     6241 gatgctcctc ctgcggaaca ggaaccgaca ggcccgtgaa gcttcacgct cactacacgc
     6301 cccgttcctg aggcccccac acgtacttgt gcaattgaag ctggaagcgc accggcagcc
     6361 ggtcggcaat gatccatccg gcgagatcgc gcggcgggag agtctcgaac gcggggctga
     6421 acagaatcgc gctcactcgc cccagttcag gatgagccgc aagaacttct ttcgcccagt
     6481 cgtaatcact cctgtccgtc agaacgaatt tgatctggtc attttcccgc agggacggta
     6541 gattcacgag atagtccccc tccgccatac cgctgccggg cgttttcacg tcgatgatcc
     6601 gtgtaacccg ttccgggact ccccgcgcgt ccacgtgtcc gtttgtctcc agaagcacct
     6661 ccagcccccg gccaatgagc gtttccatca acgggtagac tgcgggctga agaagcggct
     6721 ccccacccgt aatttccacc agcggtactc ccgatccgct cacccacgcc gcaacgtcgt
     6781 cgacagccat ggcgcgaccc gcgtctgcat cccatgcgta gttcgtgtcg caatacgcgc
     6841 accgaagatt gcatccggca aggcggacga atgcgcacag ccttcccgcg gcaaggcttt
     6901 caccctggat gctgacgaac agctcgctta cggagagtgt ttccatggtc ctatcgtttc
     6961 tgatgtcgtg caggcaaccg gtaggtaatc ctcttcacgc ataccatcgc tgaacggtca
     7021 acctgtcctc ccgccgcatc aggtgatcga gcaggcgaag gcgcatttca tctctccggc
     7081 cccggaacgc cgggtcatcc cacagattcc gtgtttcgtg cggatcgttc acgcggtcgt
     7141 acagctctcc ccacggccgg ccggcgtatg ccgtgatctt ccactccgcg gtcaccagcg
     7201 tacgcaatcg ttcaccgagg taatcgtcgt cgttttcggt caatgcctcg tcccgtgcat
     7261 acgtctcctg cccgcgcagg taccgcgcca gcgatatgcc ttcgataccc ggttcaggag
     7321 gtatcccgca cagatccagg acggtcggcg tcacgtcaag cgtcgaagca acgcccgatt
     7381 cgacgacacc cgagtgcaag gcaccgggca gattccagat gaatggcaca cgcgttaacc
     7441 catcccagtg catgggccct ttataaagga atccatgatc gccaaggaag tccccgtggt
     7501 ccgagcaaaa aacgataacc gtactccgcc gcagcccggt ctcgtcgagc gtccgcagga
     7561 tgttccctac tgcagcgtct atcagttcca ccattccgca tgtgagggcg cgggcgcgga
     7621 gcattgcagc cggcgggatg gccgagtaat caacgtggcc gccgccgctg aacatcccgc
     7681 tctgcccccg gtacgccctg ccaaaatgcg gcggaaccgt cccgaatcca tccgaatccg
     7741 gcggaaccgg aacaggcata tcaccggggt cgtaccggtc ggcatactca gcaggcgggg
     7801 cgaacggatg atgcggatcc gagaaggatg caaccaggaa aaacggcttc tcgtgcctgc
     7861 gccgaaggta gtccgacacc atctcgccga gggcggaagt gggatggagc tctgccggca
     7921 gcggtgaacg gaacatgtca gccagcccgt cttcgtatgc cccggcccgc atgtaccccc
     7981 cgtcgccgga agcgttttcg atcacacgtt ccgcggcggg atcacgttcg cgcagccagt
     8041 cccggtaatg cccccagatg cccggaccat gccccccgaa gaagaccacg ttctcaaatc
     8101 cgtagtaatc gccttcaatc gggtgtgccc cgtcgttcca gaggtgtcgc atttcgcggc
     8161 tcggacagtc cgcgtagtcg cccatcgggg caaaatgcgg cttgccgaac agcgcggttt
     8221 cgtagccgtg ctcccggaga acggcgggca acatgcgctc gctctccgga agaatcatcc
     8281 cggtcatgcg agccccgtgg gagctggggt accgccccgt gaaaatcgca cctcgggacg
     8341 gcgtacacgt gggatgagtc gtatacgcac aatcgaaccg gacgccggat gccgccagtc
     8401 cgtcaatgtg cggggtgcgg accgcagggt gcccggcgca cccgaggctg tccgcacgct
     8461 gttgatcggt catgatgagc agtacgttcg ggcgcgccag atcggtgctt tttcccgcgc
     8521 ggtctttcac ctcaggtctc ctttccgtcc acgtccaagg gaatgtaatc acccgccgat
     8581 tcgcctccaa ttcgcacaag tgcggtttgt ccgctgaccg gccgtggcta cattgcgaga
     8641 aggggaaaac ccgctctttt tgttgccgcg gagatacgca tgcatcctct tttgctccag
     8701 ctcgaccttg accacccggg actggaagag gcgaacgcgc ttgccagagc atctcgcatc
     8761 cacgaggcgc tggccgccgt agtccgttac ttccgtgcgc gtcctgaacc cgatccgtcc
     8821 ctcctcgcca caccgaaccc cggtacagtg gaggcctctg agagagccat cgcacgggag
     8881 ttcgtgttct accaggtccc cgggaaggtg ccgcccggca actgggattg gacgtggcgc
     8941 ccgggaaccg actgggagtg gacctacgcg ctcaaccggc accactggtg ggcggaactc
     9001 gcggctgcgt atctcgcaac cggcgaccaa cgttacgcgc aagaacttga ttcgctgatc
     9061 cggagctggg tggggcaatg tccggttcac gtggatgaca tctcgtcctg gcggacactt
     9121 gaaaccggaa ttcgcgtcgc ggggccatgg atcggaattc tctccgcgat gaaggcgact
     9181 cccgtcatca ccgataccgc atggatcatg tacctgcggg ccattcacga ccacgcggag
     9241 catctcgtcg cgcacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat
     9361 gcgctcacat tcgagatcat ggcgtacggc cagccgctca tcggaaccat gggacggtat
     9421 tcgtacgatc cggtgcccgt tcgcagatat ctcgtcagca gcgcagcgta caacaccgtg
     9481 atgatcgatg gtcagggcca gaaatggacg aaaacgcacc ccgaccgttc cacctgggtc
     9541 gcccgtgaac cgaccgcaga cccttggtcc agcaccgcgg agcaggatgt cgcgtacggc
     9601 cgtttcaatg gcccgtggac gggtgatttg tcgggtgttg tctgggagcg gcgcatggcg
     9661 ttccacaaac ccgacgcgaa gcttcgacgc cccgggttct gggttgtgcg ggataccttc
     9721 acgggagaag gcgagcacga actgcgcttc ctgttgcatt tttaccccgg aaatgtcgtg
     9781 tgcgacgacg aaacacatac ggtgcgatct gactactccg gggcacaggc gaacgttatc
     9841 gtcgcgtttg aacaccccga ggctgtgaag ttcgattgcg cgtacggtca ggaagaccct
     9901 ccacgaggct ggtacagcgc tcgatacggc gttatcgagc cagcgtggga ggtggcagtc
     9961 gtgcgcgccg ccgtctttcc cgcggatttc gtcatggtct tcgtgccgta tcaggctgag
    10021 gcaaaacttc ctgttcgtgt caagagggaa cagggtgtcg tgagcgtaca actcgatgac
    10081 agggaatggc tggtgtccgt ctagaaggac ttcccggcga accaaacttt ttttggagga
    10141 cctcccggta tgaaattgca caacgcgttt ctcgtcaacg ggcaaccgat tctcggcacg
    10201 tacgtcgctt ccggagtgga agagcttgcg ctgtgcaaag aaatcggcat gaacgtggtg
    10261 ataggaggcc acaccgaact cgacgtgaca accccggagg gagccttctg ccagcaaaac
    10321 ggcatcaaag tcatgcacca cctcgtccag cacatctacg gcaaaccccg cctcgcggat
    10381 ttcgtgacgc ccgcccagac ggaaatcccg tttgtcgccg atctcatgaa aggtgtcgca
    10441 caaccatcgg atgagcttcc caaaggcgtt ccgtcttccg gagtgttcca gatcgacgat
    10501 gaaaccgtgt actacgggaa gctcactgca acgcacttca cggagtgtgt tcgcgggtac
    10561 aggggaacga aaccggcgga gcatcgtgcc ggggcgttcc ttttcttccc ggaagcatgc
    10621 gcggaagaga tccggaaggt ccgcacctca ccgaatctgt ggggctacta cgttcttgat
    10681 gacagccccg gcgatgcact ctcggcgctc agagcactgt accggacagt caaagaggaa
    10741 gatccgggac gtccggtctg tgccggatac ggaagcgccg gctcactgtg caattttgga
    10801 ccggatgtgt gcgacgtgat gatgttctac tggtaccccg tcgcggaaag ctgggaaccg
    10861 gtggcccacg gacagtactt ccgcatgctg accccgcacc aggtgcaata catgctgatg
    10921 gcagcgcggg agatggttcc cgggattccg ttcgtgggcg tgtatcagtc ctttgatgcg
    10981 aaattcgacc ctgaccaaaa gaatatcgcc gtcccgacgg cggaccagat tcgcgaacag
    11041 atcgaggatt acgttcggga aggagcgtgc ggcctgatcg cgttcttgtg ctcgaaaaag
    11101 catttcgggt tcgcgcacta cccctacatg gtggaggaac tgaaggccat ccaccacgag
    11161 atactggaaa caggcgagtt gcaggtccgg ccggagaccg aagagatgaa acgaacgcgt
    11221 gtccagccag tcggtttctg ggagaagccc ctgccattgc cgggtgtcat acctgcctgg
    11281 tggacggcat tcccgttcga agacgcaaaa ggcgagatgc tggaggcaac cttcggcccg
    11341 gaccacgaat tcgaccttga cgcagtgtac cccgggaaaa cgtggccggt tcggtggacc
    11401 gtacgcagaa ccttcggagg ctctctgggc ttcggtgaac tcctgggccc tcattcgtat
    11461 acgaaaaact gcgtcgcgta cgcagtgtgc gaggtagaaa gcccgaaaca gcaggaagtc
    11521 attctttcca tcggaagcga cgaggacgcg ctgatcaaat tgaacggcaa ggaagtgtgg
    11581 cggtttgggg gtgggcgggg gctcacgcgc gatagcgaca gagtgcgtgt cacattgccg
    11641 cagggcaagt ctcgaatttt cttcaaggtg cggaacagac agggcctctg ggggctcgat
    11701 cttcgttttc tggatggcga gggtaagccg ctcgaagggc tgaagttttc gccacaaccg
    11761 taacgagtct tccggcggaa cggcaaccac gcgggttccg ttccgcgtct ctctgaatcg
    11821 cggcatttca tggaggtgtg atcagttgtt gaacctggga gtcctggcat ccggcggcgg
    11881 aacgaacctt caggcgctca tagaccgaag cgaggcaggc acactggcgg caaaagtggt
    11941 cgtagtcatc agcaacaact cgactgcagg agcgctggag cgggctcgca ggcatggcat
    12001 cccctgctac cacctcagca gggcaacatg cggcgatgaa gcctcgctgg atgcagccat
    12061 gtgtgaaaca atgcggcgcc acggggttga tctcgtcatt cttgcggggt acatgcgccc
    12121 tgtcggcccc ggcctccttc aggcgtatcc cgaccgcgtg ctgaacatcc acccggcact
    12181 gcttccaaaa ttcggcggga aggggatgta cggcatacac gttcatgaag cggttctggc
    12241 ggcaggcgac aagacaaccg gcgttactgt ccacctcgtc aattcggagt acgacagggg
    12301 cagaatactc gctcaacgaa ccgcgcccgt tctgcccggt gacacgccgg aatcgctcca
    12361 gaaacgcgtc cttgcgatcg aacatgagat ctacgcggac gttatcaaca gaatcgccgc
    12421 gggcgaaatc cgcctctgac ggcggaagcg gagcggcttc tcacgtttcg gaccgattgc
    12481 attttttcgt gcatgcgttg tactttgtga aagtctggct ccaatcggtt cgtttacccc
    12541 ctctttcatg agagcg
//
LOCUS       DAOTNG010000055        12481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4846_length_12481_cov_5.541204, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000055 DAOTNG010000000
VERSION     DAOTNG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_12481_cov_5.541204"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5179_04090"
     CDS             <1..1113
                     /locus_tag="P5179_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRS94432.1"
                     /translation="ILRKSAGAIIVTASHNPVEWNGLKFLDQEGVFLRQGQLDRLVSI
                     WKRSVPEWKRWEFVQPVLNWDGAADAHVERILALDVVNAEACRQARLPVVLDTICASG
                     GRIAPKLLRALGSPFVHVNAEITGKFTRKPEPVPENIRDVGEAVRANHAALGMVLDPD
                     GDRLALLDETGEPIGEEYTLALSIYAVRRRARTRAPVVVNASTSRMVDDVGAMFGFPV
                     LRTAVGEINVVEGMVKHGADLGGEGNGGVIYGGLHYGRDGLLGMAIILSLLAEEKCRL
                     SDLVGKLPRYVISKQTATVPPEKRAGVLHRVRSLAAEMGAELDMCDGVKLVWADRWLH
                     VRPSNTEPIIRIFAEAPTEQDARELCARAQEIAARE"
     gene            1264..1587
                     /gene="raiA"
                     /locus_tag="P5179_04095"
     CDS             1264..1587
                     /gene="raiA"
                     /locus_tag="P5179_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRS94433.1"
                     /translation="MQVFITARHCEISQTFRDRITSEVEKLTKIFERINRVEIIVSED
                     AGVNQVEINFTVNQQAFNAKGQGASHQLAFDIALEKAERQLRKFKGKLVGRRAIVAKE
                     DQTGN"
     gene            1559..2581
                     /gene="hprK"
                     /locus_tag="P5179_04100"
     CDS             1559..2581
                     /gene="hprK"
                     /locus_tag="P5179_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRS94434.1"
                     /translation="MQRRTRLATETPEQASVGITIEDLVRLHGQRLLLELLAGKAGTW
                     RRIFSAEPHRPSLPLTGFVDLYTFDRAQVLGNTELLYLRQMDPKSRYEALYIIFQFEL
                     PCVIITNAYEVFPELIQLAEEREIPLLRTSLSTARFVHHYVAALDFDFAPQTTVHGTL
                     VDVYGVGLLLTGRSGIGKSEIALDLVERGHRLVADDVVHLRRQRRVLMAEANRLLGFN
                     IEVRGIGILDVERLFGIRAIRARKRVEVQVLLTPWDGAEDYERTGLDDERSAILGVEI
                     PLVRLPIFPGKNVTVICETIAMNHLLKVRGYNAAEVLSNRLTSELNKASETVPRPSNL
                     SDLDAT"
     gene            2578..2988
                     /locus_tag="P5179_04105"
     CDS             2578..2988
                     /locus_tag="P5179_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94435.1"
                     /translation="MIRAVLVCHGELSAALLTSAEEIVGHIPEVICISNRGKSPEIVR
                     QEIIHAASENAGLSGVLVMADLFGSSCWRCAMEAARVGTGVPMAVVTGVNLGMLLSFS
                     QKRDSMDFPTLASTMAEDGRRGINGPVFSSEGGT"
     gene            2985..3464
                     /locus_tag="P5179_04110"
     CDS             2985..3464
                     /locus_tag="P5179_04110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRS94436.1"
                     /translation="MILLVRVDERLIHGQVTVAWRGALDLDAFAVVDDELAGSDWEKD
                     LVLAAAPEGTVAEVVTVADAVRLWNDWNADTRRRMVLVQSFASARSICASGVDLGSLN
                     VGGLHKREGRKEFLPYVWLDSAEMETCRYLCRSGVTLEARNVPATSGVNLCARIGDS"
     gene            3480..4373
                     /locus_tag="P5179_04115"
     CDS             3480..4373
                     /locus_tag="P5179_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRS94437.1"
                     /translation="MLSRERYEIKVGVVFILTVTCLIAGLLWAKRIRPRQSEMQLAAV
                     FSTVGGLGTGDEVLVNGLRLGKVEAIRLRQRDVLVTLTLDRRIPLYQGYNIRIITLNF
                     TGEMGVSIEPGAGEPLPEPYGVLRGTDPFSLEDVVQPGLETIRSVRSVAETLSVSLPP
                     LVHDARNVLSRLDNTLEEARSGVAVNKVALRRSLDDLQRVLGSANRTIASLETRLDTT
                     LTRADDAFLTIKTASDSLRRAAETIAQARGTIARLVHDPSLYEDLRRTSLHLDSAATS
                     IDSLAQDVRRNPKRYVRFSLF"
     gene            4486..5166
                     /locus_tag="P5179_04120"
     CDS             4486..5166
                     /locus_tag="P5179_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94438.1"
                     /translation="MTGQVDTPGTEGPYPLMVLIPGGSLSRRGTSSQFPAMSRLYDPL
                     VEAALREGWAVFRYDRHETPPGSRDESQDIIDAIRAARALPDVDTRRIVLCAHASGTV
                     FLQQAYDRVEEIVGFRSLRGVILLSNRADASEAGRMAGHLLVVVGESASTDEKRAGEN
                     AVRAHRESYPRWNARYLMVPNADVALCDTTTSNWRGWQGLPGSCHFSPEIPRAISELL
                     RAASAATF"
     gene            5388..5897
                     /locus_tag="P5179_04125"
     CDS             5388..5897
                     /locus_tag="P5179_04125"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRS94439.1"
                     /translation="MSDTTKRDGEQPTVNRRPVSATSVLNEAYHYQLPSSFSRGMRID
                     LPCVSMIYISGTASVDENGATAHVGDFEGQVRRTFRNITELLKAEGLTWKHVVRTTCY
                     LKDIQRHYDEFNRLRTEFYKQQGLDPLPASTGIEARLCRDDLLVEIEAIAMVPGPNHQ
                     GCSEYVDKK"
     gene            complement(5977..7053)
                     /locus_tag="P5179_04130"
     CDS             complement(5977..7053)
                     /locus_tag="P5179_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94440.1"
                     /translation="MAVRPRPLKSADSKPPAPVNVGIIGTGIGGAHFDGYSKLKNVRV
                     VGLVDVDPARGKSVAERWGVPVVHAFTNHHDLLSLKELDAVSVCTPNYLHAQIAIDAL
                     NAGKHVICEKPMAATLAQAKELFNVAKQSDKIFMMGYNNRYRGDTQLLKKYIEAGELG
                     DLYYGKCGWIRRHGAPGPGSWFSTKSMSGGGPLIDLGVHALDLAWWLMGKPKPVSVSA
                     SVFNLLMPQEWERLGRPGTMDVEDAAVAHIRFANGAAVLLEVSWLLHTPKESFYCQVM
                     GTKGGASAEPEFRICQDKHGAEVDLTPRAPNIGGHPAEIAHFVQCIRTNTQPFSTAED
                     GLLVMKMLDAIYRSGESGKAVTIT"
     gene            complement(7056..7850)
                     /gene="mazG"
                     /locus_tag="P5179_04135"
     CDS             complement(7056..7850)
                     /gene="mazG"
                     /locus_tag="P5179_04135"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRS94441.1"
                     /translation="MTPLEELIEVMARLRAPGGCPWDREQTHETLIPYLVEETYEAVE
                     AIERKDFDELKKELGDVLLQVIFHARLAEEAGRWNIHDVAAATVAKLKHRHPHVFGNV
                     SADTPEKVLANWEQIKQKEREGSEKESVLDGVPNHLPALRKAKRVQEKVARVGFDWER
                     TEQVVEKVEEEFQEFCEACKEKDRAKTEEELGDVLFALVNLARFLDTDPEDSLRKTVD
                     KFTRRFKHIEAGLAAKGKLPQDASLAEMDALWDEAKSLEHTQQPKE"
     gene            complement(7905..9356)
                     /locus_tag="P5179_04140"
     CDS             complement(7905..9356)
                     /locus_tag="P5179_04140"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRS94442.1"
                     /translation="MGKHSHFRQIRDCVYEAPETAQKGMRGPARIFADEQILSKAEED
                     GSLDQLLNTATLPGLVGAAMAMPDCHLGYGFPIGGVVATDARDGVVSPGGVGYDINCG
                     VRLLRTPFPAGEVREFIGALADAIFRACPSGVGSRGPRMLSAAELDDLLISGARWAVA
                     QGYGVEADLENTEDRGALDAADPDVCSHTAKKRGQEQLGTLGAGNHFIEVDYVAKLHD
                     TAAARLFGLTEGTIAIQIHCGSRGLGHQICTDSVSALQKASRSFGYTPPDKQLACAPV
                     GSEEGERYLAAMAAAANFAWANRQVLTHGVRQSFRQFLGDAVEMDDISTLYDIAHNIA
                     RMETHELGGRNVRVCVHRKGATRALPAGHPAVPERYRAAGQPVLVPGSMGTRSYVLAG
                     NPDSLLLSLGSSSHGAGRLLSRKSSKKSEGGREVFERLSSKGIVIRTDSFRGLAEERP
                     EAYKDVDTVVSVLQRAGLARIVAELHPLAVVKG"
     gene            complement(9386..10534)
                     /locus_tag="P5179_04145"
     CDS             complement(9386..10534)
                     /locus_tag="P5179_04145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS94443.1"
                     /translation="MSGRTWVVSGMAAALGGYGYAMARIVHGLTKIKPGKNDWQYSMS
                     VIVPARNEEATIRKCLEGLLEQDYPKYFYEIIVVNDRSTDSTAAIVEELRRENPRIRL
                     VNITEVPAGVAPKKNAVVTGIQHAIGRIIVTTDADCDHPKTWLTALNREFAPDVGVVV
                     GHTVYRTPRNWFEGLQAVDYLSHRIVGAGTIGEGEALNSTASNLAYRREVYDEVGGFG
                     DARGIVSGDDDLFLQRVHTMTSWRIVAPTSPESFVATQPVSSVSAFLNQRARWASKST
                     NYHAGVLPFLVATFLLLILIAFSLPFAIRRPKRWALFWALLAAKAGIDFNVMKTGSRM
                     FGQTELMKYFWLAEILMPFYVVAAVFWGLFGKFAWKGSLWGRRVAAAA"
     gene            complement(10559..12157)
                     /locus_tag="P5179_04150"
     CDS             complement(10559..12157)
                     /locus_tag="P5179_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94444.1"
                     /translation="MDPHTRDRIIPPLRRWAQPGDINAFFGLMLDNMSDLVIMAGILT
                     MAFGIPADIVLGRMIPGTAVGVLVGDLLYTWMAWRLAERTRRDDVCAMPLGLDTPSTF
                     VFCLAIIGPVFLDATARGMTPHDAGMVAWKAGMAILVLTGLVKVAVSFVGTAVRDWIP
                     RAGLLGSIAAVATVLISFLPALEVFASPIAGFLSLGIILVAVIGGRTLPFRFPAAFGA
                     VLLGGIVYYVLVALGVQSGKGWTDALAGASFHVALPIPTLAFLDGLGPALRYLPIAVP
                     MAIMTVVGGIDCTESAAAAGDEYNTRDILLVEGFATVLGGLCGGVLQSTPYIGHPAYK
                     RMGGRAAYTLATALFIGLGGVFGYLSFFVDLIPRAATAPILIFVGLEITAQAFTATPS
                     RHAPAVAIAFIPVIADLIIVELGQFLPAQNGIPPQMQGVHQTLLIMANGFIFISMVWA
                     GATAFITDRRYAAAASLFFGAAVLTSVGIMHSPFATGEMYLPWLLSGAKAHLVYTVAF
                     AYLLLAVGLFLLRWIPEQREHAAS"
BASE COUNT         2497 a         3677 c         3748 g         2559 t
ORIGIN      
        1 atccttcgca agtcggctgg cgcaatcata gttacggcaa gccataaccc ggttgaatgg
       61 aacgggctca agttcttgga ccaggaaggt gtgttcctcc ggcaggggca actggaccgc
      121 ctggtttcca tctggaaacg ctctgttcct gagtggaaga gatgggagtt cgtgcaaccg
      181 gttctgaact gggacggagc ggcggacgcc catgtggaac gaatcctcgc gctggacgtt
      241 gtgaacgcgg aggcgtgcag gcaggcgagg cttccggtgg tacttgatac gatctgtgca
      301 tcgggaggac ggattgcgcc aaagctgttg cgggccctcg gatcgccgtt tgttcacgtg
      361 aacgcggaaa tcaccgggaa attcaccagg aaaccggaac ctgttcctga gaacatacgg
      421 gatgtgggag aagccgtgcg cgccaaccac gcggcacttg gtatggtact cgatcctgac
      481 ggggatcggt tggcgcttct tgacgaaacg ggagagccga taggagagga gtatacgctc
      541 gcgctcagca tctacgcggt tcggcgccgc gcacgcaccc gcgcgcccgt cgtggtaaac
      601 gcgtccacca gtcgaatggt ggatgacgtc ggtgccatgt tcgggtttcc cgtgctccga
      661 accgctgttg gcgagataaa tgtggttgaa gggatggtga agcacggagc cgaccttggt
      721 ggcgagggga atggcggagt gatctacggc ggcttgcact acgggcgtga cggcctgctc
      781 gggatggcaa tcattctctc gcttctggcg gaagagaaat gccggctttc agatctggtg
      841 ggaaaactgc cccggtacgt gatctccaaa caaaccgcca ccgtaccgcc ggaaaaacgc
      901 gccggagtac ttcatcgtgt tcggtcgctc gccgcggaaa tgggcgcgga gctcgacatg
      961 tgcgacggag tgaaactcgt gtgggcagac cggtggctgc atgtacgccc gtcgaacacc
     1021 gagccgatca tacggatttt tgcggaggca cctaccgagc aagatgcgcg tgagctctgc
     1081 gcgcgtgcgc aggaaatcgc cgcgagagag taaggaggtg tggcggcgtc gggggaaccg
     1141 gaacgcgtgt ccgcgcatag ttgactaatg agtccgggaa ggataccttc ccgttcagga
     1201 atgccccgtg aagctcacgg ggaatctcga ggagaagtcc acccgcagca ggagagacaa
     1261 gccatgcaag tcttcatcac tgcccggcat tgcgagatca gccagacatt cagagaccga
     1321 atcaccagcg aagtcgaaaa gttgacgaag atttttgaac ggatcaatcg agtggaaatc
     1381 atcgtatcag aggatgcagg cgtaaatcag gttgagataa atttcacggt gaatcaacag
     1441 gctttcaatg cgaaagggca gggagcttct catcagctcg cgttcgatat tgcgctcgaa
     1501 aaggcagagc gccagttgcg gaaattcaag ggcaagctag tgggcaggcg cgcgattgtt
     1561 gcaaaggagg accagactgg caactgaaac tcccgaacaa gcctctgtcg gcatcacgat
     1621 tgaggatctt gttcgattgc acggtcaacg actcctgttg gagctgctgg caggcaaggc
     1681 tggcacctgg cggcgcatat tttccgcgga gccacatcgg ccgtcgcttc cgctcacggg
     1741 ttttgtggac ctgtacacct ttgaccgtgc ccaggttctc ggcaatacgg agcttctgta
     1801 tctgcggcag atggatccca aaagcagata cgaggcactg tatatcatct tccagttcga
     1861 gttgccgtgc gtgatcatca cgaacgcgta cgaggtgttc ccggagctga tccagcttgc
     1921 ggaggagcgg gagatcccgc tccttcgcac gtcgctcagt acagcgcgat tcgttcatca
     1981 ctacgttgcc gcgctggact tcgatttcgc cccgcaaaca accgtgcacg gcacactggt
     2041 ggacgtgtac ggcgtggggc ttctcctgac gggccgttcg ggcatcggga agagcgaaat
     2101 cgcccttgat ctcgtcgaac gtgggcaccg gcttgttgct gacgatgtgg tgcatctccg
     2161 gagacagcgc cgggtgctca tggcagaagc gaaccggctg cttgggttca atattgaggt
     2221 gcggggaatc gggattctgg atgtggaacg gctgttcggc atccgtgcaa tccgggcgcg
     2281 gaaacgagtg gaggtgcagg tgcttctgac cccatgggac ggggctgaag actacgagcg
     2341 aaccggtctg gatgacgaac gaagcgcgat tctgggtgtt gagatacccc tggtcaggct
     2401 gccgatcttc cccggcaaaa acgtgacggt gatctgcgag acgattgcga tgaaccatct
     2461 cctcaaagtg cgcggctaca atgccgctga ggttctttcg aaccggctca cgagtgaact
     2521 gaacaaggcg tcggaaacgg tgccgcgccc atccaacctg agcgatctgg acgccacatg
     2581 atacgcgcgg tgcttgtgtg tcacggggag ttgagcgcag cacttctcac gtctgcggag
     2641 gaaattgtcg gtcatatccc cgaagtcatc tgcatatcga accgggggaa atccccggaa
     2701 atcgtgcggc aggagataat ccacgcagcg agtgaaaacg ccggtttgtc cggagttctt
     2761 gtgatggcgg acctgttcgg gtcgagctgc tggcgttgcg caatggaagc agctcgtgtc
     2821 gggacaggtg tgccgatggc cgttgtgacg ggagtcaacc tcggaatgct tctttccttc
     2881 agccagaaac gcgacagcat ggattttccg acgctggcct cgacgatggc agaggacggc
     2941 agacgcggca tcaacggccc ggtgttttca tcggagggcg gaacgtgatt ctgctcgtgc
     3001 gtgtcgacga acggctgatc cacgggcagg ttacggtggc gtggcggggt gccctcgatc
     3061 tcgatgcatt tgcggtcgtg gacgatgagc tcgccggaag tgattgggag aaggacctgg
     3121 ttcttgcggc ggcgccggaa ggaactgtgg ccgaagtcgt cactgtggcg gacgcggtgc
     3181 gcctgtggaa tgattggaac gccgataccc ggcgtcgaat ggtccttgtg cagtcgtttg
     3241 ccagcgcccg gtcaatctgt gcctcgggtg tggacctcgg atcgctgaac gtgggagggc
     3301 tccacaagcg cgaagggagg aaggaattcc tgccgtacgt ctggcttgat agtgccgaaa
     3361 tggaaacctg ccggtacctt tgccgttcgg gggttacgct ggaagctcgc aacgttcccg
     3421 ccacgtcggg tgtcaacctg tgtgcgcgaa tcggggattc ctgagcgaaa ggtgcgtgga
     3481 tgctctcgcg tgagcggtac gagatcaaag taggcgtcgt ttttattctg acggtcacct
     3541 gcctcattgc tggcttgctc tgggcgaagc ggatccggcc caggcagtcc gagatgcagc
     3601 ttgcggcagt gttctcgact gtcggaggcc tcggtaccgg cgatgaggtg ctcgtgaacg
     3661 ggttgcgcct ggggaaggtg gaggccatac gcctgcgcca gcgcgatgtg ctcgtaacgc
     3721 tcactctgga ccgcagaatt ccgctgtatc aaggctacaa catccgcatc atcacgttga
     3781 attttaccgg agaaatgggc gtctcaatcg agccaggggc tggtgagcct ctccccgagc
     3841 cgtacggagt gctccgggga acagaccctt tcagcctgga agacgtggtg caaccgggcc
     3901 ttgagacaat ccgctcggta cggtcggtcg ctgaaacgct gagtgtttca ctgccgccgc
     3961 ttgttcatga tgcaaggaac gtcctcagcc gtctggataa cacgctcgaa gaagcacggt
     4021 ccggagtagc ggtgaacaaa gtggcccttc ggagaagcct tgacgacctt cagcgcgtgc
     4081 tcggatccgc aaatcgtacc atcgcttcgc tcgaaaccag gttggacact accctgacgc
     4141 gggcagacga cgcgtttttg accatcaaga ctgcttccga cagtctgcgg agggcagcgg
     4201 aaacgatcgc tcaggcaagg gggactatcg ccaggctcgt gcatgaccca tcgttgtacg
     4261 aggacctccg tcgcacatct cttcacctcg attctgctgc gacaagcatt gactccctcg
     4321 cgcaggacgt tcgcagaaac ccgaaacggt atgtgcgttt cagcctgttc tgatctccga
     4381 agtcaacggt tcctcctagt atgcctggcc gtcatggttt tgccgggggc tcaggcgact
     4441 gcccgtcccg ccccgcgaga cgtgcgcttc ggaacgggat cgggggtgac cgggcaagtt
     4501 gatacacccg gcacggaagg tccctaccct ctcatggttc ttattcccgg aggcagtctc
     4561 tcacggcgtg gcacatcaag ccagtttccc gcgatgtcgc ggttgtacga cccgctggtg
     4621 gaggccgcac tcagagaagg gtgggcggtt tttcggtacg atcggcacga aacaccaccc
     4681 ggcagcagag atgagtcgca ggacatcata gatgcaatca gggcggcaag ggcccttccc
     4741 gacgtggata caaggcgtat tgtcttgtgt gcacatgcct ccggcaccgt gttcctgcaa
     4801 caggcctacg acagggtgga ggaaatcgtc ggattccggt cgttgcgcgg ggtgattctg
     4861 ctttcgaacc gggcggatgc ttcggaagcg ggacgcatgg cgggccacct tctggtcgtc
     4921 gtcggggaat cagcctcgac ggacgagaaa cgcgcaggtg agaacgccgt tcgagcccac
     4981 cgcgagtcgt accctcgatg gaacgcgcgc tatctgatgg ttccaaacgc ggacgtggcc
     5041 ctctgtgata cgaccacctc caattggcgg ggctggcaag gattgccggg gtcctgccac
     5101 ttttcgccgg agattccaag agcaatatcg gagcttctgc gtgcggcgtc cgctgccacg
     5161 ttctgagaag acgtcacggg tgttgcccgg gggagaattc tcgggtaccc ttaatccgtt
     5221 gtatttttgt tgaacgccgc tgagacgcaa acggggattc gcgccacgtg gtctttttgt
     5281 cggtgtgacg agtttcttcg ttcccgccgc atttggttgc cttggcacaa tccgctccgg
     5341 atgaaccttt ctgcggtgtt tccgcacgct acgccagagg agtatccatg agcgatacga
     5401 caaaacgcga cggggaacag cccacggtca accggcgacc tgtttctgcg acatctgtgt
     5461 tgaacgaagc ctaccattac cagctcccgt cgtctttctc gcgaggtatg cgaattgacc
     5521 ttccctgcgt gtcgatgatc tacatcagcg gaaccgccag cgtggacgaa aacggtgcca
     5581 cggcacatgt cggggatttc gaaggacagg tgcggcggac cttcaggaat atcactgaac
     5641 tcctcaaagc ggaaggcctg acatggaaac atgtcgtaag aacgacctgt tacctcaagg
     5701 atatccagcg gcattacgac gaattcaacc ggctcagaac cgagttttac aagcagcagg
     5761 ggctcgaccc gttgcccgcc agcaccggga tagaagcacg tctgtgccgt gatgacctgc
     5821 ttgtcgaaat cgaggccatt gcaatggtgc cgggcccgaa tcaccaggga tgctcggagt
     5881 acgtggacaa gaaataggcg tgaagaaaca gaaacgcgaa agggcggctc accagccgcc
     5941 ctttttgttg cccttcacgc gccgcatctc cgtgcgtcag gtaatagtaa cggccttgcc
     6001 gctttcgccg gagcggtata tcgcgtccag catcttcatg acaagaaggc cgtcttcggc
     6061 agtggaaaag ggttgggtgt tcgtgcggat acactgcacg aaatgcgcga tttctgcggg
     6121 gtgcccgccg atgtttggtg cgcggggtgt gagatccact tctgcgccgt gcttgtcctg
     6181 gcagatgcgg aactcgggct ccgcggaagc gccacccttt gttcccataa cctggcagta
     6241 gaagctttcc ttaggagtgt gcagaagcca gctcacctcc agaagcaccg ccgctccatt
     6301 cgcaaacctg atgtgagcca ccgcggcgtc ctcaacgtcc attgttccgg gacgtccgag
     6361 tcgttcccat tcctgtggca tgagcagatt gaataccgat gcgctcaccg aaaccggctt
     6421 cggtttgccc atcaaccacc acgcaaggtc gagtgcgtgg actcctagat cgatcagcgg
     6481 cccgccgccc gacatacttt tcgtgctgaa ccatgaacca ggtccgggtg ccccgtgtcg
     6541 ccgaatccag ccgcattttc cgtaatagag gtcacccagt tccccggcct cgatgtactt
     6601 tttcaggagt tgagtatctc cccggtagcg gttgttgtat cccatcatga aaatcttgtc
     6661 tgattgcttt gcgacgttga acagctcttt cgcctgtgcg agcgtggctg ccatcggttt
     6721 ttcgcagatg acgtgcttcc ccgcgttcag tgcgtcaatc gcgatttgcg cgtggaggta
     6781 attgggtgtg catacgctga ccgcatccaa ttccttcagg gagagcaggt catgatgatt
     6841 cgtgaacgca tgaacgacgg gaacacccca gcgctcagct acagactttc ctcttgccgg
     6901 gtctacgtcc accagtccga caacccgcac gttcttgagt ttgctgtaac cgtcgaaatg
     6961 cgcgccgccg attcctgtac cgatgattcc gacgttcacc ggcgccggcg gttttgaatc
     7021 tgcggatttc aacggtcgtg gtctgactgc catgatcact ccttgggctg ttgtgtgtgt
     7081 tcgagagatt tcgcctcgtc ccagagtgca tccatctccg ccaggcttgc atcctgtggc
     7141 agtttgccct tggccgcaag ccccgcttct atgtgtttga accggcgtgt gaatttgtcc
     7201 accgtcttcc ggagggaatc ttcggggtcg gtgtcgagga accgagccag attcacaagc
     7261 gcgaagagga cgtcacccag ttcctcttcg gttttcgccc gatctttctc tttgcacgct
     7321 tcgcaaaact cctggaattc ctcctcgact ttttccacca cctgttccgt gcgctcccaa
     7381 tcgaatccca cccgggcgac cttttcctgt acccgttttg ctttcctcag tgcggggagg
     7441 tggttgggaa ctccatcaag cacggattcc ttttctgatc cttcgcgttc cttctgcttg
     7501 atctgttccc agttggcgag gaccttttcc ggcgtgtccg cggatacatt cccgaacacg
     7561 tggggatgcc gatgtttcaa tttcgccacg gtcgccgctg ccacatcatg gatattccac
     7621 cggcccgctt cttctgcaag ccgcgcatgg aagatgacct gcagcagcac gtcgccaagt
     7681 tccttcttca gttcatcaaa atccttgcgt tcaattgcct cgaccgcctc gtacgtttcc
     7741 tccacgaggt aaggaatgag agtttcgtgc gtttgttcgc gatcccaggg gcaccctccc
     7801 ggtgcccgca agcgagccat cacttcgatc agttcttcaa gaggtgtcat gaggtaccct
     7861 gaatgaggtt gaggggaatg caacgcgcga aggacaaggg tcggttatcc tttaaccacg
     7921 gcaagaggat gaagctcggc aacaatgcgg gcgagccctg cccgctgcag tacggagacc
     7981 accgtgtcaa cgtctttgta ggcttcgggt cgttcctccg caagcccgcg gaacgaatcc
     8041 gttctgatga ctatgccttt cgacgagagc ctttcgaaca cctccctccc cccctcactt
     8101 tttttcgagg attttcggga gagcagcctt cccgcgccgt gcgagcttga accgagactc
     8161 agtagaaggg agtctggatt tccggcgagc acgtaggaac gcgttcccat ggaacccggg
     8221 acgagcaccg gctgcccggc cgcccgatat cgctccggaa cagccggatg acccgccggc
     8281 aaggctcgcg tcgcaccttt acggtgcacg cacactcgaa cgttccggcc gcccagttcg
     8341 tgtgtttcca tcctcgcgat attatgcgcg atgtcgtaca gggtgctgat atcgtccatt
     8401 tccaccgcgt cacccagaaa ttgacggaaa gactggcgta ctccgtgggt aagaacctgc
     8461 ctgttggccc acgcgaaatt tgccgcggcc gccatcgcgg caaggtaccg ttccccttcc
     8521 tcgcttccca caggcgcgca cgcgagctgt ttgtccgggg gagtgtatcc gaagctccgg
     8581 gaggctttct gcaacgcgct tacggaatcg gtgcagatct ggtgccccag gcctcgcgat
     8641 ccgcaatgga tctggatcgc gatggtgcct tccgtcagcc cgaacaggcg tgctgctgcg
     8701 gtatcatgga gtttcgcaac gtaatcgacc tcaataaaat ggttgccggc accgagtgtt
     8761 ccgagctgtt cctggccccg ctttttcgcc gtgtggctgc agacgtcggg atccgcagca
     8821 tcaagcgcgc cccggtcttc cgtgttctcc agatcggctt ccacgccgta tccctgcgcg
     8881 accgcccacc gcgcccccga tatcaggagg tcgtccagtt ccgccgccga tagcatccgt
     8941 ggacctcgcg agccgactcc gctgggacac gctcgaaata tggcgtccgc aagtgcgcca
     9001 atgaactccc tcacctcacc agcaggaaat ggcgtgcgca ggaggcggac cccgcagttg
     9061 atgtcgtatc ccactccgcc gggggacacc acgccgtcgc gcgcgtccgt tgccacgact
     9121 ccgccgatgg gaaacccgta cccgaggtgg caatcgggca tggccatggc ggcgccgaca
     9181 agacccggca acgttgcggt gttcagcaac tgatcgagag aaccatcctc ctccgctttg
     9241 ctcaggatct gctcgtccgc gaaaatccgc gcgggaccgc gcataccttt ctgcgccgtc
     9301 tccggtgctt catacacgca atcgcggatc tgccggaagt gagaatgctt tcccatggat
     9361 gctcaaccgc gcatggtggc gtgattcatg ctgctgctgc cacgcgacgc ccccacaacg
     9421 aacctttcca cgcgaatttc ccgaacaagc cccagaaaac cgccgcaacc acgtagaacg
     9481 gcatgagaat ttccgcgagc cagaagtact tcatcaattc cgtctgcccg aacatgcggg
     9541 aaccggtctt catgacattg aaatcgatgc ctgcctttgc ggcaagaagc gcccagaaga
     9601 gtgcccaccg tttcgggcgg cgaatcgcaa atggcaacga aaaggcgata agaatcagta
     9661 acagaaaggt tgcgacgagg aatggaagca ctccggcatg gtaatttgtg gatttggatg
     9721 cccaacgggc acgctggttc aggaaggcag acacactgga aaccggttgt gtggcaacaa
     9781 aagattcggg agatgtcggt gcgacgatgc gccatgaagt catcgtgtgc acgcgctgga
     9841 ggaagagatc gtcgtcgccg cttacgatac cgcgtgcgtc tccaaacccg ccaacctcat
     9901 cgtacacctc cctgcggtac gcgagattgc ttgcggtgct gttcagggcc tcgccttcgc
     9961 cgatagttcc cgcgccgacg atcctgtgcg agaggtaatc caccgcctgg agcccttcaa
    10021 accagttgcg aggagttcga tacacggtgt gtccgacaac cacacctacg tcaggggcaa
    10081 attccctgtt caatgccgtc aaccaggttt ttgggtgatc gcagtcggcg tccgttgtaa
    10141 cgatgatgcg cccgattgca tgctggattc cggtgacgac tgcgttcttt ttgggtgcaa
    10201 cgcccgccgg tacttcggtg atgttcacga gccggattcg cgggttctcg cggcgcagtt
    10261 cctccacgat cgccgcggta ctgtcagtcg aacggtcgtt caccacgatg atttcataga
    10321 aatacttcgg gtaatcctgc tcaagcaggc cttccaggca tttccggatt gtggcttctt
    10381 cgttcctcgc cggaacgatg acgctcatgc tgtactgcca gtcattcttg ccgggcttga
    10441 tcttcgtcag accgtggacg atacgtgcca tcgcgtaccc gtatccgccc agtgccgcgg
    10501 ccattccgct tactacccat gtgcgtcctg acatgaactg attctcccct ttgcgtgctc
    10561 aggaagcggc gtgctcgcgc tgttctggaa tccaccgcag cagaaacagt cctaccgcga
    10621 gaagcagata cgcgaacgcc accgtgtaca ccaaatgcgc tttcgccccg gacaaaagcc
    10681 aggggaggta catctcgccg gtcgcgaatg gcgaatgcat gattccgacg ctcgtaagca
    10741 cggccgcgcc aaaaaacagg ctggctgcgg ctgcataccg gcggtccgtg atgaatgccg
    10801 tggcacccgc ccagaccatt gaaataaaga taaacccgtt tgccatgata agaagcgttt
    10861 gatgcacccc ctgcatctgc ggaggaatgc cgttctgtgc cggcaaaaac tggcccagtt
    10921 cgacgatgat caggtccgcg atcaccggaa tgaaggcgat cgccactgct ggtgcgtggc
    10981 gtgacggcgt cgcggtgaat gcctgtgcgg tgatctcaag tccgacaaaa atcaggatcg
    11041 gggcagtcgc cgccctcggg atcaggtcga cgaaaaaact cagatatcca aacacgcctc
    11101 caagcccgat gaacagcgcc gtcgccagtg tgtacgccgc tctccccccc atcctcttgt
    11161 atgcgggatg gccgatatac ggcgtggatt gcagaacgcc gccgcaaagc ccgcccaata
    11221 cggtggcgaa cccttccaca agcaggatgt cgcgcgtgtt gtactcatcc cccgccgccg
    11281 ccgcgctttc cgtgcaatcg attccgccga cgaccgtcat gatggccatg ggtacggcga
    11341 ttggcagata ccgcaatgcc gggcccagtc cgtccagaaa cgccagtgtg gggatcggca
    11401 gtgccacatg gaacgaggcc ccggccagcg cgtcagtcca ccctttgccg ctctgaacgc
    11461 ctagcgcaac caggacgtag tacacaatcc cacccagaag cactgcgccg aacgcagcgg
    11521 ggaagcggaa cggaagcgtc cttcccccga ttactgcgac gaggatgatt cccaggctga
    11581 gaaaacccgc gatcggagag gcaaacacct ccagcgcggg gaggaacgag atcagtaccg
    11641 tggcaacggc cgcgatagaa ccgagcaatc ccgcccgggg aatccagtct cgcaccgccg
    11701 tcccgacgaa cgatacggca accttcacga gacccgtgag gaccagaatc gccatcccgg
    11761 ctttccatgc gaccattccc gcgtcgtggg gcgtcattcc tctcgcggtg gcgtcgagga
    11821 acaccgggcc gatgatcgcg aggcagaaca cgaaggtgga tggtgtatca aggccgaggg
    11881 gcatggcgca gacgtcgtcc cgccgggtgc gttccgcaag ccgccatgcc atccaggtgt
    11941 agagcaggtc gcccaccagc acaccgaccg ccgtgccggg gatcattctt ccgagcacga
    12001 tgtccgccgg gattccgaac gccatcgtga ggattcccgc cataatgacg agatcgctca
    12061 tgttgtcgag catcagtccg aaaaacgcgt tgatgtcgcc cggctgcgcc cagcggcgaa
    12121 gcggcgggat gattctgtcg cgagtatgtg ggtccacggg aacagactcc tgtggcgtgg
    12181 gtgtgatcgg atgccccgaa ttccgcctcg atcaatcgtc cggggaatgg tcaaaatagc
    12241 gagttcgtca tgcaaacacc acgggggatg gcgtggcgag ccgccgtcgt gcccatcttc
    12301 cgccacaaac ggaacgagaa ccgcgcaaaa cgtaggcccg ccgccggcat ttgttctttt
    12361 gcctccgtgg gccgggcgac ccggccccta cacccacatc gtgaccgcta cacgcccggt
    12421 tggaggggca cgctccgtcg tgcccgattt ttgccgcgca cgaaatacct ccccacacac
    12481 c
//
LOCUS       DAOTNG010000056        12343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_4920_length_12343_cov_5.404541, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000056 DAOTNG010000000
VERSION     DAOTNG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4920_length_12343_cov_5.404541"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5179_04155"
     CDS             complement(<1..347)
                     /locus_tag="P5179_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94445.1"
                     /translation="MDYSYGEWEHQDQPRRTRFWTGVLLGIGVSVVALFIANRITGRH
                     WGGRFSVPEPPAAFSLVSGVGDTTGDVANSRRNAIVRAVEVVAPAVVTISVTQLRRER
                     AVPFADDPMWQFF"
     gene            complement(352..1086)
                     /gene="truA"
                     /locus_tag="P5179_04160"
     CDS             complement(352..1086)
                     /gene="truA"
                     /locus_tag="P5179_04160"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRS94446.1"
                     /translation="MSTRLRLDMEYEGTEFAGWQVQPGLRTVQGELNTALSTILRTPV
                     RVIGAGRTDAGVHARGQVGHADVPDAYPPLARILAGVRALTGPDLLVREITVVPGDFH
                     ARFSARARTYEYRIALVPSPLERRFQWFVPYRLDVGSMSVAAQKLEGRYPATCWCSSS
                     APDTTAIVHVQRTEIERRATEIVFRITSDRFVTHMVRTIVGTLVEIGRGVRSPEDIGA
                     IISSQDRARAGPTAPAHGLCLQSVEY"
     gene            complement(1083..1841)
                     /locus_tag="P5179_04165"
     CDS             complement(1083..1841)
                     /locus_tag="P5179_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HRS94447.1"
                     /translation="MNFDLNIGRYVAGTSPIHRLDPRTKLGLALFLCIGILQGTSATA
                     TAWHCVLATVLFGISGLKARFVARNLRPFVYLAGMILASHLLINGAGSWQTGISVGVR
                     LLVMVSVVSLFSWTTQPLATVAGLRRLGSPLARLKVPVDAAATSIGLAMRFAPIVVSE
                     AQTVLRAQAARGADFRGVKRKLTLIVPLLGALFDRSFARAEVLAEAMQARGYDPSAVR
                     TSYHRLGFRGVDVIALAYVAIWLGGAVIMERYAP"
     gene            complement(1911..2654)
                     /locus_tag="P5179_04170"
     CDS             complement(1911..2654)
                     /locus_tag="P5179_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS94448.1"
                     /translation="MRISSLATGCIVAISFVALGCPAPPPATVPAAGDTQNNDTTATT
                     TAPELPDPRGQLPRTATPAPAEATDGPDAEPASELESAVYSVQVMSSSSRDAAEAAAR
                     QVRSLTSQPVEVVQESGGAWRVYAGRSGTRAPMDRLRDQLISAGYSGAWTKQRVVETS
                     VRDNTIPTGESVYSVQIFVSSTPENARRVAAEARSVTRLPVEVVEMDGYWKVFVGRSP
                     TRSAIDAERDALRQRGYPDAWTYRRQGTR"
     gene            complement(2681..3916)
                     /locus_tag="P5179_04175"
     CDS             complement(2681..3916)
                     /locus_tag="P5179_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRS94449.1"
                     /translation="MRERFGFIASALAAFLFVGCPAPPRYAPTSRLAPRPGELPVRIG
                     LAEATGSVRIEADQGLRLVAGETVRLAPSFALSPSRGHIAVNGARTPHKRVTCFASTP
                     LSCNGVPYRGQLEVSAVEGALRVVNIVGLEEYLAGVVPAEIGRLSTREIEAVKAQAIA
                     ARTYAVANRGRRRAQGFDLYADVRDQVYMGVRGEHAVAARAIEQTRGQIVTYRGEPIQ
                     AFFHSTCGGFTASIEDVWNADPLPYLKGVPDRDSHGFHCETSRHFRWSEEYSPGVLRT
                     ILSENLFREVPNAPRKIGTVRKIEAPERSSSGRVRYLKVETNRGHWRVSKDRIRWALR
                     RPVDGNPILRSTYIRIFSEYDAHGSLQRVVISGAGNGHGVGMCQWGAIGMARKGYPAE
                     QILKHYYRGVTITSMRMAD"
     gene            complement(3991..4494)
                     /locus_tag="P5179_04180"
     CDS             complement(3991..4494)
                     /locus_tag="P5179_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRS94450.1"
                     /translation="MPGYETLKTASLLKGLTDSQLKKLWDAGESKTIKAGDTIIREGE
                     VGDTMYVLFEGTVEVSQVLVLRLGNAAIAEKDKTLIRLEGTARPCFGEMSLLEDAERS
                     ATITALTDCQVFVIDRSSFNRLVEEDPVMGTKLLRSIAVVLSGRLRKANKDVLKLTTA
                     LSLALTD"
     gene            complement(4526..5923)
                     /locus_tag="P5179_04185"
     CDS             complement(4526..5923)
                     /locus_tag="P5179_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRS94451.1"
                     /translation="MFSLAGSAGGVVVLAFVGAAFFSRRHGRKGSGSSGTPRIRTASP
                     GPAASAPEPASARTVIAAVAKPFQSLAAGVVSLKREVWKENIPRLAGMVFLLLAVGAL
                     VVGLVEVLFGAENQRANFGSPINALYWAVVTLATVGYGDLAPQTTAGRLLTSVFILVG
                     MVTISVFTATLASVFTAKRIKEGRGLEKVKATNHVVVCGTGRHLDTIIRYLTRAGITR
                     KQPIVLVNSLTEEAMNEILYRYSHLDIQHVHGDFTQESVLRRANIEDAVAAIIQAGDD
                     TQDRSRADNRTLQAALAIKALNHDIKLTAEALDPENEAHLRRANVDDVIVSGEFSGYL
                     LGACTVSPGLDVALRELLTVEVRNDMIRNPIPESFRGKTSKELSEWFRRREAMLIGII
                     VEQKVLNLDEMLQDDFSSIDRFIRSTFSAAGKELVAQGKGRTEVVVNPPDDRVIGPDD
                     AAIIISPRVIPVTET"
     gene            complement(6226..7320)
                     /locus_tag="P5179_04190"
     CDS             complement(6226..7320)
                     /locus_tag="P5179_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRS94452.1"
                     /translation="MKLGPLLFEPIYRSYVWGNRNFERLFGRVLPPEVPTAESWELSD
                     VDGAISRVESGPFAGRSLRELCQEFAADMFGEQSGSSICGGRGFPLLVKFLDAERVLS
                     VQVHPGDQFAAEHENGSIGKTEMWFIVEARGNAEVIAGLRPGTTRAEFLEAAQSGSLE
                     PLLQHHCVRAGDVVLVPAGRVHAMGAGIVALEIQQTSDITYRLHDWGRVGPDGKTRPL
                     HLDRAIEVIDFSDEGRAVTQPVQRTEDWGVWSLLGVTLFFLTESFSQFRSVPCTTRSG
                     SPDILVAMNGSLRISWDHGDVIVPPGRTALVPSCVGLYVLEATEDTLVVRSRVPDRSD
                     VLGDWTPRSNEERTAVLDVCDTRVAELVTA"
     gene            complement(7317..7712)
                     /locus_tag="P5179_04195"
     CDS             complement(7317..7712)
                     /locus_tag="P5179_04195"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRS94453.1"
                     /translation="MAEQDAKLTQEQQDTFAFMAMVELYQQTAWMALGKIADPATKET
                     KANLPQARWAIDTLAMIERKTRGNLSDQELRFLREILHMLRMNYVEEANKPVGEPPST
                     QDKTETKPEKETATHDEPETPPPAEPQNE"
     gene            complement(7735..8388)
                     /locus_tag="P5179_04200"
     CDS             complement(7735..8388)
                     /locus_tag="P5179_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRS94454.1"
                     /translation="MIQNELLQTLKTEAYTAVVADVLDSLGLQNQILPSRIRPLGNSE
                     AVVGIARTILVRDVVSASPHPYEVEFALVDDLREGDVIVAQCGEREAAFWGELLSTAA
                     AKRGAVGAVVDGFCRDLRQISELRFPVYARGMSPADSKGRCEAVARDIPIEIGGVAIH
                     PGDMIFADLDGVVVVPAGSIDETARGALEKVRGERTVFNALQSGMSTREAYDKWGIL"
     gene            complement(8395..8970)
                     /locus_tag="P5179_04205"
     CDS             complement(8395..8970)
                     /locus_tag="P5179_04205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRS94455.1"
                     /translation="MGHSKAMVLSPDAEWRWHVRADMPPPRVGEVTHLLKPHGECVLT
                     ILTSQQSVWLHTKDFYPAGCYRLPTGGIKPEEHPDGAARRELREEVGCAGSCRILRLG
                     KITYVEASGTAYPFVSYLYQVAPVEKTPTSADESERISGWKTVPISHLGAVASELRAL
                     PAEWVDWGCFRAVAHDAVREILRREAQSPCL"
     gene            complement(9225..9860)
                     /locus_tag="P5179_04210"
     CDS             complement(9225..9860)
                     /locus_tag="P5179_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRS94456.1"
                     /translation="MELFVLGVPGNRMRFRKIASAEAIERFFRQIRTCCALAEGFREP
                     FEGQIECDESTFGGARHGKRGWGAAGKIIVFGILQRNGKIQAFPINRRQGAEVIQLVA
                     EHTRPGSLYYTDDWQAYASLAIRGGHIVVTKEGGKPKGRDHINGIEGFWSYAKHWLYP
                     YRGVPQKFFHLYLAEICFRFNHRREDLCPIFTNMLQKTPSSLTLTTLVRSD"
     gene            complement(10485..11240)
                     /locus_tag="P5179_04215"
     CDS             complement(10485..11240)
                     /locus_tag="P5179_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94457.1"
                     /translation="MWLDRIAPFAFVFLGAVFFGCAPTCVKTPPPPAGELIAEVLSVP
                     GAHAIQAYGTLRVTSPEGNYSGSAVVFYSYPESVKVVLQGGFGTTFAEIALAGGKGIA
                     YLPQQRQALELADGSGLLFGSVVVYPSMLLHLLKPVDAERLGQDAIISTECSQYVLAS
                     RTAAGTRIWRLSARARRLEAEEFRSVENTMFWTRTFGAANGIRVPKSFSVRLGETTMS
                     AEFTRINVSPVMRPNTFAVALPPGIEVMAVPRE"
     gene            complement(11227..11907)
                     /locus_tag="P5179_04220"
     CDS             complement(11227..11907)
                     /locus_tag="P5179_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94458.1"
                     /translation="MVRVLGGLLAVLSAVLSARASDWAPGAGINVVTAHVVRGDKRNG
                     LSLQPAFWFNAFQERGLFTSWASVAPADPALDDLVLDFRAGVPTAGWGWMGGVVHWDW
                     SRPPSDSLRSHSLEIGPTLTWFKPPYRPSLTVTYDFAQRNGVLFTVNAPYTVRMTWLP
                     ATTFTPEVAFRFAGAGNRVSAEYFSFSALLERKIRGVTLLPTAGIVPRSELSKSLVWA
                     GIHVNVVR"
BASE COUNT         2541 a         3856 c         3539 g         2407 t
ORIGIN      
        1 aaaaagaact gccacatggg gtcatctgcg aacggaaccg cgcgctctcg gcgcaactgc
       61 gtcacactta tggtgacaac cgcaggagcg accacttcga ctgcccgaac aatggcattc
      121 cggcgagaat tggcgacgtc tcccgtggtg tcacccacac cggaaacgag gctgaacgcg
      181 gccggcggct ccggaacgga gaagcgcccg ccccaatgcc gtccagttat gcggttcgca
      241 atgaacagcg ccacgacgct gacgccgata ccaagcaaaa ctccggtcca gaaccgggtg
      301 cggcggggtt gatcctggtg ctcccactca ccgtacgagt aatccatagg ctcaatattc
      361 cacggattga agacacaggc cgtgggctgg cgcggtcggc ccggcacgcg cacggtcctg
      421 cgaagaaata attgcgccga tatcctccgg gctccgaacg ccgcgcccga tttccacaag
      481 cgtacctacg atcgttcgca ccatgtgcgt cacgaagcgg tcggaggtaa tgcggaacac
      541 gatttcagtt gccctgcgtt caatttccgt acgttggaca tgcacaatcg ctgtcgtgtc
      601 cggcgcgctg gatgagcacc aacaggtcgc cgggtatcgg ccttccagct tctgcgccgc
      661 gacactcatc gagccgacat caagccggta cggcacaaac cactggaacc ttcgctccaa
      721 cggggatggc accagcgcga tccggtactc gtatgtccgc gcccgcgcgg agaaccgggc
      781 atggaagtct cccggaacaa ccgttatttc ccggacaagc aggtccggcc cggtcagcgc
      841 tctgacaccc gcgagaatgc gcgcaagagg cgggtacgcg tcaggaacat ccgcgtgacc
      901 tacctgtccg cgcgcatgaa cacccgcatc tgttctcccc gccccgataa cgcgaacggg
      961 cgttctcaat atggtcgaca gagcagtgtt gagctcgcct tgaacggttc gcaaccccgg
     1021 ttgaacctgc cagcccgcga attcagtgcc ttcgtactcc atgtcgaggc gcaggcgagt
     1081 actcacggtg cataacgctc catgatgacc gcgcccccga gccagattgc cacgtacgcc
     1141 agggcaatca catcgacgcc tcgaaacccg agtcggtggt acgatgtcct gacggctgac
     1201 ggatcgtacc ctcgcgcctg catcgcttcc gcaagcacct cggcgcgcgc aaacgagcga
     1261 tcgaacaacg ccccgagaag cggaacaatc agcgtaagtt tccttttcac gccgcggaaa
     1321 tccgcacccc gtgcggcctg cgcacgcaac accgtctgcg cttccgaaac cacaatgggc
     1381 gcgaagcgca ttgcgagtcc gatggaggtt gccgccgcat caaccggaac cttcagcctc
     1441 gccagaggcg agcccagccg acgtaacccc gccaccgtcg ccaggggctg tgtcgtccag
     1501 ctgaagagcg agacaacaga aaccatgacg agaagacgca caccgacgga gattccggtc
     1561 tgccagctgc ccgcgccatt gatcaggagg tggctcgcca gaatcatgcc ggcaagatac
     1621 acgaacggcc ggagatttct cgcgacaaac cgcgccttca ggccactgat gccgaacagg
     1681 accgttgcca gcacgcagtg ccacgccgtt gcggtggcgc tggttccctg caaaatgccg
     1741 atgcaaagga acaatgcaag tccgagtttc gtccgggggt cgagccggtg gatcggactt
     1801 gttcccgcaa cgtacctgcc gatgttcagg tcgaagttca tgcgccagtt acccgcgcag
     1861 gtgcaaatgt accacgggaa atcgccaaga aacaaccgca gaatggcgtt tcatcgtgtt
     1921 ccctggcggc ggtaggtcca tgcgtccggg tagcctcgtt gccgcaatgc atcgcgctcg
     1981 gcgtcaattg cactccgcgt gggactccgc ccgacgaaca ctttccagta cccatccatt
     2041 tccaccactt ccaccggaag gcgggttacg gaacgagctt cagcggcaac ccgccgtgca
     2101 ttctccggag tcgatgacac gaaaatctga acgctgtaca cgctttcccc agttgggata
     2161 gtattgtcac gtaccgaggt ttccaccacg cgttgtttgg tccacgcacc ggaataccct
     2221 gccgaaatca attgatccct gagccggtcc atcggagccc gcgttccaga cctgcccgcg
     2281 tatacccgcc aggctccccc ggattcctga accacttcaa ccggttgcga ggtgagtgag
     2341 cggacctgtc gtgcggccgc ttcagcggca tctcgcgagg aagagctcat cacctgaacg
     2401 gaataaactg cggactccag ctcgcttgcg ggttcggcgt ccggcccatc ggtcgcctcg
     2461 gcaggagccg gcgttgccgt gcgagggagc tggccgcgtg gatcaggcag ttccggagca
     2521 gttgtagttg ccgtggtatc gttgttctgt gtatcacccg ccgcaggcac cgttgcgggc
     2581 ggcggcgccg ggcatccgag cgcaacgaac gagattgcca cgatacaccc tgtcgcgagc
     2641 gacgagatac gcattgctgc tccacctccc ggtcgtgtct tcaatctgcc atcctcatgc
     2701 tggtaatggt gacgcctcga tagtaatgct tcaggatctg ctccgccggg taccctttcc
     2761 gcgccatccc gattgcgccc cactggcaca tgcccactcc atgcccgttt ccggcgccgc
     2821 ttataaccac tcgctgaagc gagccgtgcg cgtcatattc agaaaagata cgaatgtagg
     2881 tgcttctcag tatcgggttg ccgtccacag ggcgcctgag cgcccagcga atgcggtctt
     2941 tggagacccg ccagtgaccc cgattggtct ccaccttgag atatcgaacg cgcccgctcg
     3001 aagatcgttc cggcgcttcg attttcctta cggtgccgat cttccgcggt gcgttgggta
     3061 cctcacggaa caggttttca gacagaatcg tgcgcagaac acccggggaa tactcttctg
     3121 accatcggaa atggcgactc gtttcgcaat ggaacccgtg ggagtcgcgg tctggcacgc
     3181 ccttcagata cggcagcggg tcagcgttcc agacgtcttc tatgctggcg gtgaaccctc
     3241 cgcacgtgct gtggaaaaac gcttgtatgg gctcgccgcg gtacgtcacg atttgccccc
     3301 gggtctgttc gatggcgcgc gccgcgacag cgtgttcccc gcgaacgccc atgtaaacct
     3361 ggtcacggac gtcggcgtac aggtcgaatc cctgcgcccg gcgacgcccc cggttcgcta
     3421 ccgcgtatgt tctggccgca atcgcctgtg ccttgacggc ctcgatctcg cgggtgctga
     3481 ggcgcccgat ctctgcggga accacgccgg cgaggtactc ttcgagaccc acgatattca
     3541 ccacacgcaa cgcaccctcg accgcggaaa cctccaactg gccacggtac ggtacgccgt
     3601 tgcagctcag gggagtggat gcaaaacagg taacacgctt gtggggtgtc ctcgcgccgt
     3661 tcacggcaat atggccgcga gagggagaca gcgcaaacga tggagccaat cgcacggttt
     3721 cgccggcgac gagacgcagc ccctgatcgg cttcgattcg gacgcttcct gtcgcctccg
     3781 caaggccgat gcgaaccgga agttcccccg gacggggagc caaacgagag gtaggcgcat
     3841 aacggggagg ggcggggcag ccgacaaaaa ggaacgcggc gagtgcactc gcgatgaagc
     3901 cgaagcgttc gcgcatcagc cccgatcttc tccggcggaa cgggctggca gtgacggcct
     3961 catggcgtgt ccgggcgagt gtcggatgat tcaatctgtc aacgcgaggc ttagcgccgt
     4021 agtaagtttc aggacatcct tgttggcttt gcgcagacga cctgaaagaa cgaccgcaat
     4081 acttctcagc aatttggtac ccatcaccgg gtcttcctca accagtcggt tgaagctgct
     4141 ccggtcgatg acgaaaacct ggcagtccgt cagtgcggtg atcgtggctg accgttcagc
     4201 gtcttcgagc aacgacattt ccccaaaaca agggcgggct gttccttcaa ggcgtatcag
     4261 cgttttgtcc ttctcggcga tggcagcgtt cccaagtcgc aacaccagta cctgggaaac
     4321 ctccacggtt ccctcgaaca ggacgtacat ggtgtcgccg acttcgcctt cccggatgat
     4381 ggtgtctccg gctttgattg tcttgctttc gccagcatcc cagagcttct tcagctggga
     4441 atcggtcaat cccttgagca gggaagctgt tttcagagtc tcgtatcccg gcatcaggta
     4501 ctcccccgat cggaaatggg cctggtcatg tttctgtgac gggaatcacg cgcggcgaaa
     4561 taatgatagc cgcatcgtcg gggcctatta cgcggtcgtc tggaggattg accacaacct
     4621 ctgttctgcc tttgccttgt gccaccagtt cttttccggc ggcagaaaaa gttgaacgga
     4681 tgaaccggtc tatggacgaa aaatcgtcct ggagcatttc atccaggttc agcactttct
     4741 gttcaacgat tatccctatc agcatggcct ctcggcgtcg aaaccactca ctcagctctt
     4801 tggaagtctt gcctcggaac gattcgggga tcgggttccg gatcatgtcg ttccggactt
     4861 ccacggtcaa gagttcgcgc agcgcaacat ccaaccccgg tgacacagta catgctccca
     4921 gcagataacc ggagaactcg ccgctgacga taacgtcatc aacgttcgcc cggcggaggt
     4981 gcgcttcgtt ttccggatcg agcgcctcgg cggtcaattt gatatcgtga tttagcgcct
     5041 tgattgccag tgccgcctga agtgtgcggt tatcggcgcg cgagcgatcc tgagtgtcgt
     5101 cgcccgcctg gatgatcgcg gcgaccgcat cttcgatgtt cgccctccgc agaacgcttt
     5161 cctgagtgaa gtccccgtgc acatgctgaa tatccaggtg cgagtagcgg tagaggattt
     5221 cgttcatcgc ttcttcggtc agcgagttga ccagcacgat cggttgtttt ctggtaatcc
     5281 ccgcgcgggt gaggtaccgg atgatcgtgt cgaggtgacg tccagtgccg cacacgacca
     5341 catggttcgt agccttgacc ttttccaatc cccgcccctc cttgatcctt ttcgccgtga
     5401 aaaccgaagc gagcgtcgcg gtaaacacgg atatggtaac cattcctacg agaatgaaga
     5461 ccgaagtcag caggcgccct gccgttgtct gcggggcaag gtccccgtac cccacagtcg
     5521 caagggtaac aacagcccag taaagcgcat tgatggggct gccgaagttc gctcgctggt
     5581 tttcggcgcc gaacaggacc tccacgaggc caactaccag cgcaccgacc gccagcagga
     5641 gaaacaccat gcctgccagt cgcgggatgt tttccttcca cacctcccgc ttcagcgata
     5701 cgacacctgc cgcgagagac tggaacggct ttgcgaccgc cgcgatcacc gttcgggcgg
     5761 atgccggctc gggcgcggaa gcggcggggc ccggcgacgc ggtccggatg cgcggtgtgc
     5821 cgcttgagcc agaacccttg cggccgtggc ggcgtgagaa aaacgccgcg ccgacgaaag
     5881 ccaggactac aacgcctccc gcggacccgg caagcgagaa cattgtcgga aaaatcctcc
     5941 caaccaaggt ctgcggagca gtttggtggc cgccacccgt tgatgcgaag ccagctcccc
     6001 ttggagggac tgctgctgtg tggttgcgtg gcgcatgtac ctcggaagca gcgcttgcca
     6061 cgccgaggaa cagacagccg ctgcagaaga ccagcaacaa cccccaacgc cggaattgcc
     6121 ggggtttccc gaatacggtc tggatgaagc gcgttcgtgc catttgggct ccgcgaggaa
     6181 tcagcgtgac caacagacga tcccagatta cacgcgccgg ccagattatg cggtgacaag
     6241 ctccgcaacc cgcgtgtcac atacgtcgag aacggccgtc cgttcctcat tgcttcgcgg
     6301 tgtccagtcg cccagcacat ccgaacgatc aggcacccgg gagcgcacca ccagtgtgtc
     6361 ttcggtcgcc tccaacacgt agagaccgac gcacgaaggt accaatgccg ttcgccctgg
     6421 aggaacgatg acgtcaccgt ggtcccacga aatcctcaga cttccgttca tcgccacgag
     6481 aatatccgga ctgccagaac gcgtggtaca cggaaccgac cggaactgag agaacgattc
     6541 cgtgaggaag aatagagtga ctccgagcaa cgaccaaacg ccccagtcct ccgttcgttg
     6601 aaccggttgc gtaactgcgc gtccttcgtc cgagaaatca atcacttcga tcgcccggtc
     6661 cagatgaagc ggccgcgttt ttccgtccgg tccaacgcgt ccccaatcgt gcagtcggta
     6721 ggtaatatcc gacgtctgct ggatttccag cgccacaata ccggctccca tggcgtgaac
     6781 gcggcctgcc ggaacaagaa cgacatcacc ggcgcggaca cagtgatgct gcaggagtgg
     6841 ttcgaggctg ccggactgcg cggcctcgag gaactccgca cgcgtggttc caggccgcag
     6901 accggctatc acctcggcgt ttccgcgagc ttcgacgatg aaccacatct cggttttccc
     6961 gatagacccg ttttcgtgtt ccgcggcgaa ctggtcgccg gggtgcacct gaacactcag
     7021 cacccgctcg gcgtcgagga atttcaccag caggggaaat ccgcgaccgc cgcagatgga
     7081 agagccactc tgctcgccga acatgtccgc ggcgaattcc tggcaaagct cccgcagaga
     7141 acggccggcg aatggaccgg attccacacg ggaaatcgca ccgtcaacgt cggaaagctc
     7201 ccacgattcc gcggtcggca cttctggtgg aagaacgcgg ccgaacaggc gctcgaaatt
     7261 ccggttcccc cagacatacg atcggtatat cggttcgaaa aggagcggtc cgagtttcat
     7321 tcgttctgcg gttccgccgg cggaggtgtt tccggttcgt cgtgcgtcgc tgtttccttt
     7381 tccggtttcg tttcggtttt gtcctgagtt gacggtggtt ctccaaccgg tttgttcgct
     7441 tcttccacat aattcatgcg caacatgtgg aggatctcac gcaaaaagcg gagctcctga
     7501 tcagaaaggt tgccccgggt tttgcgttcg atcattgcga gcgtgtcgat tgcccagcgt
     7561 gcctggggga ggttcgcctt cgtttctttc gtagccgggt cggcgatttt tcccaaagcc
     7621 atccaggcag tctgctggta gagctccacc attgccatga acgcaaacgt gtcttgctgc
     7681 tcctgggtaa gctttgcatc ctgttcggcc atacctgaac tcctcagcgt atcgttacag
     7741 aattccccat ttgtcatacg cttcgcgcgt tgacatgcct gattgcagcg cgttgaaaac
     7801 ggttcgttcc cctcggacct tttccagcgc gccgcgcgcg gtttcgtcta tggacccggc
     7861 gggcactacg accacgccgt ccagatctgc gaaaatcatg tctcccgggt gtatcgctac
     7921 accaccgatt tcgatcggaa tatcccttgc aacggcttcg cagcggcctt tggagtccgc
     7981 cggactcatc ccgcgggcat acacggggaa ccgcaattcc gaaatttgcc gaaggtcgcg
     8041 acaaaagccg tccaccaccg cgcccacggc gccgcgcttc gccgcggcag tgctcaaaag
     8101 ctcgccccag aacgccgctt cgcgctctcc acattgcgcc acgatgacgt cgccttcgcg
     8161 caggtcgtcc acgagggcaa actcgacttc gtagggatgt ggagacgccg acaccacatc
     8221 ccggacgagg atggtgcgcg ctatcccgac cactgcctcg ctatttccga gtgggcggat
     8281 gcgcgagggc agaatctggt tctgcagacc gaggctgtca agaacgtccg cgaccaccgc
     8341 ggtgtacgcc tcggttttga gcgtctggag caactcgttt tgtatcatgt tccctcacag
     8401 gcagggggac tgcgcttcac gccgcagtat ttcccgcacg gcatcgtggg caaccgcccg
     8461 gaaacaaccc cagtctaccc attcggcggg tagcgcacgc agttcagacg ctacagcacc
     8521 aaggtgcgaa atcggaacgg ttttccatcc cgatatgcgt tctgattcgt cggccgaagt
     8581 tggcgtcttt tcgaccgggg ctacttgata tagataactt acgaacgggt aggcagttcc
     8641 actcgcttcc acgtaggtga ttttcccgag gcgcagtatc ctgcatgaac ccgcgcagcc
     8701 cacttcctcc cgcagctcgc ggcgggccgc cccgtccggg tgttcttccg gttttatgcc
     8761 cccggtgggc aaccggtaac agccagcggg gtagaagtcc ttcgtgtgca accagacgga
     8821 ttgctgcgaa gtcagaatcg tcagtacaca ctccccgtgc ggcttgagca ggtgggtgac
     8881 ctcaccgaca cgaggcggcg gcatgtcggc acgtacgtgc caacgccatt ccgcgtcggg
     8941 tgaaagtacc atcgctttcg aatgtcccat ggaagagacc tttgccaccc tcgtttcatc
     9001 gtattggggg gcgcctgaag cccggaggtt gttgtctcag gggttcgtac ctccgggggg
     9061 cgcgctcccc atctgggcgc gtaccccgaa ctggtgcaca aggcgtccac cctgatgcgc
     9121 tgcatcaaca acaacgagcg aacacacgca gtacaccacc aggaacacga tgcagaacac
     9181 gcgcatcggt cgtcgaacgg tgttctcttt cctgaaggta attcctagtc agatcggact
     9241 aacgtggtga gagttaggga tgaaggtgtt ttttgtaaca tatttgtgaa tataggacat
     9301 aggtcttcgc gtcgatgatt gaagcgaaag cagatttctg ccaggtagag atggaagaac
     9361 ttttggggca ctccccggta agggtagagc cagtgtttcg catagctcca gaaaccctct
     9421 atcccgttga tgtggtcccg ccctttgggc ttgccgcctt cttttgttac cacaatgtgc
     9481 ccaccccgta tggcaagcga agcatacgct tgccagtcgt cggtgtaata caggctcccc
     9541 gggcgggtat gctctgcgac cagctggatc acctccgctc cctgacgtcg attgatcggg
     9601 aatgcctgga tcttgccgtt tctctgaagt atcccaaaaa cgatgatctt tcctgccgca
     9661 ccccagccac gcttgccgtg gcgcgctccg ccaaaggtgc tttcatcgca ttcgatctgc
     9721 ccctcaaagg gctcacgaaa accttctgca agcgcacaac aggttcgaat ctggcgaaag
     9781 aaccgctcta tcgcttctgc cgacgcaatc ttgcgaaaac gcatccggtt acccggaacg
     9841 ccgagcacaa acagctccac gagcttgcgc ttcgtggcgt cggaaagtcg gctgcttgcc
     9901 cacagggaac gcaccgtata acgagtgcca cacttgcggc acttacgccg gccgtcagaa
     9961 agctgccaac tccaaccatt gctacaaaca ggacacctgg ccatacggac tccataacat
    10021 aatctagtcc gttttgccta ggaattacct tcctgaaata gtctaccgcg acacggatag
    10081 cgaacacggc gaacaacagg gtgaaaatta ccgccgttgc ctctgcggat tcctcatgct
    10141 gctcgatgac cgaggcggcc tgcggcgcag tgattcgcgc gatggttgcc gcgtgttccc
    10201 ccgtccatac ggccacccag gcggcaagta cacccagtgc catgagaacc agaacacaac
    10261 tgcgcaactg aggcctgtcg tttttggaga ttgcccccat caacaggaag agcggcgcaa
    10321 cgagcaacag cgcgaccgga aaatgtacga caagcggatg caaggcgcct ggatcggacg
    10381 gaacgttcgg tagcacggat cttcctcctg gctcagttcc cggccatttc aggaaacgga
    10441 cttacctttt gcccggtcac gtggtttctg catgccgccc ccgattactc gcggggaaca
    10501 gccatcacct cgatgccggg gggcaacgca acggcgaagg tattgggtcg catcaccggc
    10561 gacacgttga tgcgcgtaaa ttcagcgctc atggtcgttt ctcccaaccg gactgagaat
    10621 gatttcggca cgcggatgcc gttcgccgcg ccgaacgtcc gggtccagaa catcgtattc
    10681 tctacggacc ggaactcctc cgcctccaga cggcgcgcac gtgccgagag gcgccagatg
    10741 cgcgttccgg cggcagtgcg agaggcaagg acgtactggc tgcactccgt gctgattatc
    10801 gcatcctgtc cgagccgttc ggcatcaacc ggcttcagga gatgcaggag catggacggg
    10861 tagaccacta cggacccgaa cagcaagccg cttccgtctg ccagttccag tgcctgccgt
    10921 tgctggggga gatatgcgat tcccttccct cctgcaagcg cgatttcggc gaaggtggtg
    10981 ccaaatcctc cctgcagaac aactttcacc gattcgggat agctgtagaa cacgacggct
    11041 gatccgctgt aattcccctc gggtgatgtc acacgcagcg ttccgtacgc ctgtatggcg
    11101 tgtgcgcccg gaacagacag aacttccgca atgagttcgc ctgccggagg gggcggggtt
    11161 ttcacgcatg tgggtgcgca cccgaaaaac accgcgccca ggaaaacaaa tgcgaacgga
    11221 gcaattctat ctaaccacat tgacgtgaat tcctgcccag accagggatt tcgagagttc
    11281 gcttctagga acgattcccg ccgtcggcag cagggtaacc cctcggattt tgcgttcaag
    11341 caacgcggaa aacgagaaat actcagcgct cacacgattg cccgctcccg caaaacgaaa
    11401 tgcaacctcc ggggtgaacg tcgttgccgg taaccatgtc atgcgaacgg tgtaaggggc
    11461 gttcaccgta aaaaggaccc cgttccgctg cgcaaagtcg tacgtcaccg taaggctggg
    11521 gcggtacggc ggcttgaacc atgtcagggt cggacctatt tcgagggaat gtgagcgcag
    11581 cgaatcagac ggcgggcggc tccaatccca gtgcacaact ccgcccatcc atccccagcc
    11641 agcggtcggc accccggccc ggaagtcgag caccagatcg tcgagggccg ggtcggcggg
    11701 cgcaaccgaa gcccaggaag tgaaaagacc acgctcctga aacgcgttga accagaacgc
    11761 gggttgcagc gacaatccgt tgcgtttatc gcctcgaacc acgtgtgccg taacaacgtt
    11821 gattccggcg cccggcgccc agtcggaggc cctcgcagac agaacagccg acaggacggc
    11881 caggagtcct ccgagaactc gtaccattgc agttccttgt gattcttctg cgaccttgcg
    11941 accgggcgtg aaacgctgaa cacaaaaata ggcgtctccc agacgggcac acaacaccgg
    12001 gccggagagc ggacggattc aagtattaaa tgaacgagtg cggccgattt ttctctcttt
    12061 tgattcgggc agaacttcgg tgcggcgatc ctctgtcaat gcgcaaaata acgtcattgc
    12121 cgctgtttgc ccacgtatta aaagtttacg aaatatgcgg cgcacttttg cggccggatc
    12181 aatctgaaaa aaaaccttga caccgcggtg gggattgggt atatcattca ccatgaggac
    12241 gaaaagagga gatccggaaa cgaacgcaac ggcggcgcga gggcactgat cgcgagactg
    12301 cgtgtcaagg gcttgagggg cccgtcggcg aggtggacac ggc
//
LOCUS       DAOTNG010000057        12141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5006_length_12141_cov_4.764769, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000057 DAOTNG010000000
VERSION     DAOTNG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_12141_cov_4.764769"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /gene="pheT"
                     /locus_tag="P5179_04225"
     CDS             <1..1481
                     /gene="pheT"
                     /locus_tag="P5179_04225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRS94459.1"
                     /translation="FICDGERPVALGGIMGGLNSEITDDTTDVLLEAAYFTPTRIRRT
                     AKAVGLQTEASQRFERGADWDAVIEAIDWAAALIQQLAGGSVAKGRIDAYPKRLEPAA
                     ITLRWAQIPRVLGADVPREESKRILTALGIPASAETPEGLRCEQPSWRPDLTREIDLV
                     EEIARIWGFDQIPSRVGGVGVPPEEAPPELAIVPQLRSWCIGVGLQEVITSSLVAPRY
                     ADLFCGDGAKVEITNFSTADMSVLRTHMLPSLLDVARINYSRKIEGVALFELGHVYSR
                     KAEDEYNQRRVLSILLTGSTGQVRWADQRREWDFYDLSGIAETIVQRCSLRSPEIVHY
                     DGDEFVPGTGARVLVAGKEVGQLGQVRNDVCELFDLPALAYYCQFDVIGLAACQATLS
                     RVKPLPRFPAVERDLALVVPESVESQMLENTIKEAGGALLERVIVFDVYRGPGVSKGE
                     KSVAFHLDFRAADRTLKDEEVDTFMRRIVESAQGKAGARLRE"
     gene            1478..1744
                     /locus_tag="P5179_04230"
     CDS             1478..1744
                     /locus_tag="P5179_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94460.1"
                     /translation="MMQRNALEADVEAVLTRYDTVAREVMIQLARWASERAEWQAERE
                     ELQEALERAHERDARLQAAPLQEALQRAISRVDGILAAAEEVES"
     gene            1741..2055
                     /locus_tag="P5179_04235"
     CDS             1741..2055
                     /locus_tag="P5179_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRS94461.1"
                     /translation="MNGIPDSRANAITVRIYGTEYSFRSPQDPDYVKTVADTVDQEMR
                     RLSENRVVKSTLEVSVLASMHLADELLQTRKKLHEVLQRVDQTTEAMAKILDAGPSEA
                     AG"
     gene            2190..3758
                     /gene="rny"
                     /locus_tag="P5179_04240"
     CDS             2190..3758
                     /gene="rny"
                     /locus_tag="P5179_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRS94462.1"
                     /translation="MDINPIVFAIGTLALVALAFVLGWVIARRVEQAKIGSAEDAAAR
                     ILDEAQREAASLKQKVQLEARNQQLEMQERNRKEADAQRADLIRMEARLVERETKLDR
                     KSDELTAREGELNGIRKALEVRQTSLSKKEEDLNRLISEQTTALERIAHMTADEAREV
                     LIANMESKARAEGARRAKEIRDRAEQDAEREAREIIGIAIQRFAGEHTTETTVSVVQL
                     PNEEMKGRIIGREGRNIRAFEMATGVDVIVDDTPEAVILSGFDPVRREVARIAMGKLV
                     EDGRIHPGRIEELVDKARNQVEEEAQRAGEEVVYELGIHDMHPQLVKTLGKLKYRTSY
                     GQNVLQHSKEVGILTGLMAEQLGLDPLLARRAGLLHDIGKAVDRENEGTHVALGLELA
                     KRYGENEEVQASIEWHHGDPVAKSIISVLVNAADTISSVRPGARRETIEGYVKRLERL
                     ESIAQAFDGVEKTYAIQAGREVRVMVKNDRVDDAMAQQLANDIAEKIQADMEYPGQIK
                     VVVIRESRSVSYAK"
     gene            3771..4589
                     /locus_tag="P5179_04245"
     CDS             3771..4589
                     /locus_tag="P5179_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRS94463.1"
                     /translation="MRVLFVGDIVGRAGRRAAAVLIPRLRVEKNIDAVVVNAENAAAG
                     AGINPKIAEALWESGADIITTGNHAWDKREGLPLIESGRLLRPANYPSGVTGVGSMVA
                     RTKKGEALGVLCLQGRVFMKAIDCPFRTARREIESLHAETVSIVVDFHAEATSEKLAM
                     GWYLDGDVAAVVGTHTHVQTADERVLPHGTAYITDVGMTGPFDSVIGMDTKTAIQRLI
                     TGLNPRLVPAKNDVRLSGVIIDIDGATGLSTGIERVNVHYAHAGGTLSDDEESD"
     gene            4589..5467
                     /locus_tag="P5179_04250"
     CDS             4589..5467
                     /locus_tag="P5179_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRS94464.1"
                     /translation="MPAQLLDGKAASAGIRREVLSRAQHLSPAPSLCAVLVGDDPASA
                     LYVRNKERACAESGIRSVFRRLPASTTQAELLDLVRELNADQDVDGILIQSPLPRGLD
                     EAGLVNAISPWKDVDCFHPENVGLLVAGTPRFLPCTPAGVQRLLILNSIETAGKHVVV
                     VGRSNIVGKPMANIMLQKTSGANATVTVCHTGTRDIGYYTRQADILIVAAGRPNTITG
                     DMVKAGAVVVDVGMNEVEDASAPRGKRFVGDVDFHAASEVASWITPVPGGVGPMTVAI
                     LMENTLRAAELRDMRR"
     gene            5477..6697
                     /gene="xseA"
                     /locus_tag="P5179_04255"
     CDS             5477..6697
                     /gene="xseA"
                     /locus_tag="P5179_04255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRS94465.1"
                     /translation="MTHPEVVLGADALTVSEITAQLKELITSSFGVLWVTGEVSNFRR
                     QASGHWYFTLKDAGAQLRCVMWRSSTARLSFVPQDGMQVLARGSLDLYEAYGQHQLMV
                     SYLQPAGIGALQQAFEQLKSRLAEEGLFDNARKRPLPRWPKVVGVITSGTGAAVRDII
                     TVISRRMPTTRIIVRPAIVQGAEAAADVAAAIDEMNRHGAADVLIVGRGGGSLEDLWA
                     FNEEHLVRAIAGSKIPVVSAVGHEIDFTLADFAADVRAPTPSAAAEIVVPDQTEIRTY
                     VTGMLGEMVSGMRDRVTRSADQVHRAWSADVPRRLTTRVDRMSQDVDRLVETAGIRFE
                     RLVRQRRNAFGKAAARLDALSPLKILGRGYAVCEREPDGTLITSATDVDVKDRFRVRL
                     HRGIILGKVEGRLG"
     gene            complement(6694..7224)
                     /locus_tag="P5179_04260"
     CDS             complement(6694..7224)
                     /locus_tag="P5179_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRS94466.1"
                     /translation="MADETIDPRYAGCPQKHAGSSQKPNAPRPIEGVVITSIKENVDD
                     RQSLSVYLKASDPFFAGFGQSYVTKTGRGVVKAWHYHLRQTDVWYVAVGKIKVGLFDA
                     RKDSPTCGVANEVIMGGGANVTLAIPPGVFHGYLTLTEEAVLINTTNTAYDPSDEYRA
                     DWNDPRLPFVWEVVNR"
     gene            7338..>8386
                     /locus_tag="P5179_04265"
     CDS             7338..>8386
                     /locus_tag="P5179_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94467.1"
                     /translation="MIRPGRLTRKTAALSVGRGAGVAAQFALNMFLVRVVSQHEFGWF
                     HEIRVVLTFAALLDLGIPLGLLQVAGAAEPGRRHGLYRTALAIAGAAGLATGAILFLA
                     GLLPAFTGLSSAFAWSGLLIAATIVAAVLESALVVEERHAAIAAISATSAVGGLVLAL
                     AGVLWRPSISVAYAALALAACARAVALAKVTGVARTGVQWRPLAGAVQLLKTSIAVSG
                     HRALGIASGTIDRAVVGIFFSAGTLGWYVTGAWEVPFMSVFFSAIASAILPEMSAHWT
                     AKKNADVLAIWHRSIALSAWLIFPIWLWSWIWAPELLRVLFTRQYEDGLPVFRIYLLA
                     LPVRIAVYSALLVAM"
     assembly_gap    8387..8486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8487..8914
                     /locus_tag="P5179_04270"
     CDS             <8487..8914
                     /locus_tag="P5179_04270"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRS94468.1"
                     /translation="LALPVRIAVYSALLVAMEKSRVLVNGAVIDVAVNLVLSVVLAVA
                     IGPLGPAVATVVATYSQAGYYVASVRGDLPVQRVGFFPWGSLFWALTAAGLVTLPTLV
                     LRRVFEGDLPRAGIALAWTACILAAGAFFALRKRGTEGE"
     gene            complement(8943..9704)
                     /locus_tag="P5179_04275"
     CDS             complement(8943..9704)
                     /locus_tag="P5179_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS94469.1"
                     /translation="MERRYEHLRPADIIRLREAFPLAYVPLGTLEWHGPHNPVGLDGL
                     KAHALCLRAAEGGGGIVFPVVWYGEHRESHLMEINASVGKEIVTRMGLDPDDFKPGYT
                     QSGSILAQAQDYTALLWKICCQVKSLGFKGLIFLNGHYPLTHYGRFVGHLAARHLGLA
                     HWAGHEGQILEEAGEPGHGDHAGKWETSLLMALLPDEVNLQALSDAGEFIGCGRDALE
                     STAEEGEYWARRIVAALIAKGRDLLPEFAGGSTSV"
     gene            9860..10273
                     /gene="folB"
                     /locus_tag="P5179_04280"
     CDS             9860..10273
                     /gene="folB"
                     /locus_tag="P5179_04280"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRS94470.1"
                     /translation="MAKRVRAPSSVPADISHAPDVVRLRGMQFYGFHGATPQERKIGQ
                     RFEVDVEMAIDAEKVVDGDLSTTPDYRIVYGICNACVTERRYRLIEVMAREIAHEILA
                     TFPMVGVRVTVRKPCVPLGGVVTVGAEAVVQRGNL"
     gene            10270..10821
                     /gene="folK"
                     /locus_tag="P5179_04285"
     CDS             10270..10821
                     /gene="folK"
                     /locus_tag="P5179_04285"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRS94471.1"
                     /translation="MNRSRCFLALGSNLGDRRENLRQALARVSCGGDLEIVAVSRLYE
                     TVPVGPPQGPYYNAVAEIVTGLAPEELLARCRSAENAGGRQRTVRWGPRTIDIDILDC
                     GGMVRETAELRLPHPEMARRAFVLVPLRDVAPDWVHPVEHRSVGELIEALGDTGGACV
                     VIEGSGWFVPPLGMRGEGASSCG"
     gene            10812..11675
                     /gene="panC"
                     /locus_tag="P5179_04290"
     CDS             10812..11675
                     /gene="panC"
                     /locus_tag="P5179_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRS94472.1"
                     /translation="MRMITTVAEMRQWSDHERQRGTRIGFVPTMGALHAGHLSLVARA
                     RTKADVVAASIFVNPTQFGPREDFASYPRTLERDVDLLAQEGVAVVFAPSTEEMYPSG
                     CITTVRVEGLSAKLEGAIRPSHFAGVTLVVAKLFHIVQPHVAVFGRKDAQQAIILQRM
                     VRDLDFGVELDVAPTVREADGLAMSSRNVYLSIAERKQAVVLARALQTGFRAWSEGER
                     KSAVVRALMMNVLAEEPAARLDYLEIVSQRTLEPVALVRSGTLVAGAIRVGKTRLIDN
                     WWVREDGEAEF"
     gene            11752..12132
                     /locus_tag="P5179_04295"
     CDS             11752..12132
                     /locus_tag="P5179_04295"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRS94473.1"
                     /translation="MDALPTYVTMCRAKLHNATVTETKLQYEGSITVDQNLLDAAGMY
                     LYEKVHVVNLNNGERFETYIIPGERDTGEVCLNGPAARLAEVGDTVIVIAYSFVPEAD
                     APAFRPTIVVLGPQNQLREILRPA"
BASE COUNT         2373 a         3424 c         3929 g         2315 t
ORIGIN      
        1 tgttcatctg cgacggggag agacccgtgg ccctgggcgg tatcatgggg gggctgaatt
       61 cggagatcac tgacgacacc acggatgtgc tgcttgaggc ggcgtacttc actccaacgc
      121 gcatccgtcg aaccgcgaaa gcagtggggc tgcagacaga ggcatcacag cgtttcgaac
      181 gcggtgcaga ctgggacgcg gtcatcgaag cgattgactg ggccgcggcg ctgattcagc
      241 agcttgccgg tggctccgtt gcaaagggaa gaatagacgc gtacccgaaa aggctggaac
      301 cggctgcgat aacgctccgg tgggcgcaga taccccgtgt gctgggcgca gatgttccgc
      361 gggaggagtc gaaacggatc ctcacggctc ttggcatacc ggcaagcgcc gaaactccgg
      421 aagggcttcg gtgtgagcaa ccctcctgga gaccggattt gacccgggaa atagacctgg
      481 tggaggaaat cgccagaatc tggggctttg accagattcc gtcgagagtc ggtggcgtgg
      541 gagtgcctcc ggaagaggct ccgccggagc tcgcaatcgt gccgcagctt cggtcgtggt
      601 gcattggtgt ggggcttcag gaggtgatca cgagcagcct ggtggcgccc cggtacgccg
      661 acctgttctg cggcgacgga gcgaaggtcg agataacgaa tttctccact gccgacatga
      721 gcgtgctccg aactcacatg cttccaagcc tgctcgatgt tgcgcgaatc aactacagcc
      781 gcaaaatcga aggggtagca cttttcgaac tcgggcacgt gtacagcaga aaagccgagg
      841 atgagtacaa tcaacgccgg gtgttaagta ttctgctgac cgggtcaacc ggtcaggtcc
      901 gctgggctga tcagcgccgc gagtgggatt tctacgacct ttccgggatc gccgaaacga
      961 ttgttcagcg ctgttctctt cgttcgccgg aaattgtaca ttatgacggt gacgagttcg
     1021 tgccgggtac gggcgcacga gttctcgttg cgggcaaaga ggtgggtcaa ctggggcagg
     1081 ttcggaatga cgtgtgtgag cttttcgacc tccccgcatt ggcgtattac tgccaattcg
     1141 atgttatcgg tttggcggcg tgccaggcaa ctctgagccg cgtgaagccg cttcctcgct
     1201 ttccggccgt ggaacgcgat ctggcgcttg tggttccgga atcggtcgaa tcacagatgc
     1261 tggagaacac tatcaaagag gcgggcggcg cgttgcttga gcgcgtcatt gtgtttgacg
     1321 tataccgggg ccccggcgtg agcaagggtg aaaagagtgt cgcgtttcat ctcgatttcc
     1381 gtgcagcgga caggacgttg aaggacgagg aagtagacac gttcatgcgc cgcattgtgg
     1441 aatcagcgca ggggaaagca ggagccagat tgcgggaatg atgcaacgaa atgctcttga
     1501 agccgatgtg gaggctgttc ttacgcggta cgacactgtg gcccgtgaag tgatgatcca
     1561 gctcgcccgc tgggcgagcg aacgggcgga gtggcaggcg gagcgcgagg agttgcagga
     1621 agcactcgaa cgagctcatg agagggatgc acgtttgcag gcggcaccgt tgcaagaagc
     1681 gctccaacga gcgatttccc gggtagatgg cattctggct gcggcagaag aggtggagtc
     1741 atgaacggga ttccggattc cagggcgaat gcgattaccg ttcgtatcta cggaacagaa
     1801 tattcgttcc gttcgccgca agaccccgat tacgtcaaaa ccgttgctga tactgtcgat
     1861 caggaaatgc gccgtcttag tgaaaaccgg gttgtgaagt ccacgctgga agtgtcggtt
     1921 ctggcgagca tgcaccttgc cgacgaactg cttcagacgc gaaagaaact gcacgaggtg
     1981 ttgcagcgcg tggaccaaac gacggaggcc atggctaaga tactcgacgc ggggccttca
     2041 gaagcggctg gttgacagcc tcggggccga gcgccactcg ttgaaccccc tcttccgcga
     2101 aagcgcgtgc tgttgccatg cgcgcatgac ggaacagggg ttttttattt tcgcactcgg
     2161 gcaaagttgt ttcccggagg atacagggga tggatatcaa tccgatcgtg ttcgcaatag
     2221 gaactcttgc gctggtcgct ctcgccttcg tactcgggtg ggtgatcgcg aggagagtgg
     2281 aacaggcaaa aatcggcagc gcggaagacg ctgctgcgcg aattctggac gaagcgcagc
     2341 gggaagccgc gagtctgaag cagaaagtcc agctcgaggc ccgcaatcag cagctggaga
     2401 tgcaggaacg caatcgaaaa gaggcagatg cccagagagc agatttgatc agaatggaag
     2461 cccgcctggt ggagcgggaa acaaaacttg accgcaaaag cgatgaactc actgcccgcg
     2521 agggcgaact caacggcata aggaaagcgc ttgaagtacg tcagacaagc ctgtcgaaaa
     2581 aggaagagga cctcaatcgg ctcatctccg aacagacgac ggcgctcgag cgaatagctc
     2641 acatgactgc ggatgaagca cgagaggtgc tcatcgccaa catggaaagc aaggcccgcg
     2701 cggagggtgc gcggcgtgcg aaagaaatcc gtgatcgtgc agaacaggat gcggagaggg
     2761 aagcacggga gatcatcggg attgctatcc agcgctttgc cggggaacac acgaccgaga
     2821 ccacggtttc tgtggttcag ttgccgaatg aagagatgaa gggacggatt atcgggcgcg
     2881 aaggtcgcaa tattcgcgcg tttgagatgg caaccggcgt ggatgtgatc gtggacgata
     2941 caccggaggc ggtgattctt tcggggttcg atcccgtgcg ccgcgaagtg gccaggattg
     3001 ccatgggaaa actggtggaa gatgggcgca tccaccccgg gcgtatagag gaattggtgg
     3061 acaaagcgcg gaaccaggtg gaggaagaag cgcagcgtgc cggtgaagag gttgtgtacg
     3121 agctcggcat ccacgacatg caccctcaac tcgtcaagac gctgggcaaa ctgaaatacc
     3181 gcaccagtta cggacagaac gttctgcagc actcaaaaga ggtgggaatc cttaccggtc
     3241 tgatggcaga gcaactgggg ttggatcctc tgcttgccag gagggcaggc ctgctgcatg
     3301 acataggaaa ggccgtcgac agagaaaacg agggcacgca cgttgctctc ggattggaac
     3361 ttgccaaacg atacggcgaa aacgaggaag tccaggcgag tatcgaatgg caccacggtg
     3421 atcctgttgc gaagtccata atcagtgtgc tggttaacgc ggcagacacg atttcgagtg
     3481 tgcggcccgg cgcgcgaagg gaaacgatcg aagggtacgt gaaacggctg gagcgcctcg
     3541 aatcgatcgc ccaggcattt gatggcgtgg aaaagaccta cgccattcag gcggggcgtg
     3601 aggttcgggt gatggtgaaa aacgaccgag tggacgacgc gatggcgcag caacttgcca
     3661 acgacatcgc cgaaaaaata caggcggaca tggaataccc cggccagatc aaggtcgtcg
     3721 ttatccggga gtcccgttcc gtttcctacg cgaagtgagg accgttccgc atgcgggttc
     3781 tttttgtggg ggatatcgtc ggccgggcgg ggcgccgtgc cgctgccgtg ctgattcccc
     3841 gccttcgggt tgaaaagaac attgatgccg tggtggtaaa tgccgaaaat gcggcggccg
     3901 gtgcagggat caacccgaaa attgcggaag ctctctggga atcaggtgcc gacatcatca
     3961 cgaccgggaa ccatgcctgg gacaaacgag aggggctgcc tctcattgaa agtggccgtc
     4021 ttctccgccc cgcgaattac ccgagcgggg tcaccggcgt gggctcgatg gtggcacgca
     4081 caaaaaaagg cgaggcgctg ggcgtcttat gcctgcaggg gcgggtcttc atgaaggcca
     4141 tagactgccc gttccggacc gccaggcgcg agatcgaatc cttgcacgcc gaaacggtat
     4201 ctatcgtggt ggattttcac gccgaagcaa cttcggagaa acttgcgatg ggctggtatc
     4261 ttgacggtga cgtggctgca gtagttggta cgcatacgca tgttcagacc gcagacgagc
     4321 gcgtgctgcc acatggaacc gcgtacatca cggatgtcgg gatgacaggc ccgtttgatt
     4381 cggtgatcgg aatggacaca aaaacggcca tccagcgtct gatcacgggt ttgaatcccc
     4441 ggcttgtccc cgccaaaaac gacgtgaggc tttctggagt gatcattgac attgacggcg
     4501 caaccggtct ttccaccggc atagaacgtg tgaacgtgca ttatgcgcac gcaggtggta
     4561 cactttcaga cgatgaggag tcagattaat gcctgcgcaa ctgcttgacg gcaaagccgc
     4621 ctccgctggc atccgacgtg aagtcctctc acgggcgcaa catctgtcgc cggccccaag
     4681 cctgtgcgcc gtccttgtgg gtgacgaccc tgcgtccgcg ctttatgtcc gaaacaagga
     4741 acgcgcatgc gcggagtccg ggatacgttc cgtgttccgc cgtcttcctg ccagcacgac
     4801 gcaggcggaa cttctcgacc ttgtgcgcga actcaacgcg gatcaggatg tggacggtat
     4861 cctcattcag tcgccactgc cgcgtgggtt ggatgaagcg gggctggtga acgcgatctc
     4921 gccgtggaag gatgtggact gctttcatcc ggagaacgtg ggtctactgg tggccggtac
     4981 gccgcggttc cttccgtgta cccccgccgg tgtgcaaagg ctgctgatac tgaactcaat
     5041 tgaaaccgcg ggcaagcacg tcgtggtggt ggggcgttcc aacatagtcg gcaaaccgat
     5101 ggccaacatc atgcttcaaa agacgtccgg agcgaacgcg accgttacgg tctgccatac
     5161 cggcacccgc gatatcgggt attacacgcg gcaggcggac atcctcatcg tcgcggcagg
     5221 gcgcccgaac acgatcaccg gcgatatggt caaggcgggt gcggttgttg tggacgtcgg
     5281 catgaacgag gtcgaggatg catcggcacc gcgcgggaaa cgattcgttg gtgacgtgga
     5341 cttccatgcc gcgagtgaag tcgcgagctg gattacccct gtgccggggg gagtcggccc
     5401 gatgacggtg gcgatactga tggaaaacac attgcgtgcc gccgaactgc gcgacatgag
     5461 gcgctgaccc caggtgatga cacaccccga agttgttctt ggcgcggatg cgctcaccgt
     5521 aagcgagatc accgcgcagc tcaaagagct catcacgtca tctttcggtg ttctctgggt
     5581 taccggcgaa gtgagcaact tccggcgcca ggcgtctggc cactggtatt tcacgctcaa
     5641 agacgcagga gcgcagctgc ggtgcgtgat gtggcgctcc tcaaccgcaa ggctctcatt
     5701 tgtgccgcag gacggcatgc aggttctggc tcgtggaagc ctggatctgt acgaggcata
     5761 cggtcagcac cagttaatgg tgagctacct tcagccggcc ggcatcgggg ccttgcaaca
     5821 ggcgttcgag cagctcaaat cgcgcctggc ggaggagggg cttttcgaca acgcgcgaaa
     5881 acggcccctg cccagatggc caaaagtcgt tggcgtcatc acttccggga cgggagcggc
     5941 ggtccgggat atcatcaccg taatcagccg tcggatgccg acgacgcgca tcatcgtccg
     6001 tccggcgatc gtgcagggag cggaagcagc ggcggacgtg gcggcggcga ttgacgaaat
     6061 gaaccgtcac ggtgcagcgg atgttctgat cgtgggtcgg ggaggaggtt ccctcgaaga
     6121 cctctgggcc ttcaacgagg aacacctggt tcgcgcgatc gccggctcga agatcccggt
     6181 cgtgtcggcg gtgggccacg agatcgattt cacactcgca gactttgcgg cggatgtgcg
     6241 agcgccgact ccctccgccg ccgccgaaat cgttgtgcct gaccagaccg aaatccgaac
     6301 gtatgtcact ggtatgctcg gagagatggt ttcaggcatg agagacaggg taacccgctc
     6361 ggcagatcag gtgcaccgcg cgtggagtgc cgatgttccg cgccggctga cgacgcgggt
     6421 ggatcggatg tcacaggatg ttgaccggtt ggtggaaacc gcgggcatcc ggtttgaacg
     6481 ccttgttcga cagcgacgaa acgcgttcgg gaaagccgct gcccggttgg atgccttgag
     6541 cccgctcaag attctcgggc gcgggtatgc ggtctgcgaa cgcgaaccgg acggaacgct
     6601 cattaccagt gcgaccgatg ttgatgtgaa agaccgtttt cgcgtgcgcc tccaccgcgg
     6661 catcattctg gggaaagtgg agggacgcct cggctaacgg ttgaccacct cccacacgaa
     6721 aggcaaccgg gggtcattcc agtccgcgcg gtactcatca gaagggtcgt acgcggtgtt
     6781 ggtcgtgttg atgagcacgg cttcctcagt gagtgtcagg tatccgtgaa atactcccgg
     6841 gggtatcgca agcgtcacgt tcgccccgcc tcccattatg acttcatttg caaccccgca
     6901 cgtgggcgaa tctttccgcg catcaaagag accgactttg atcttgccga ctgccacgta
     6961 ccagacgtct gtctggcgaa gatggtaatg ccacgccttc actacgccgc ggccggtttt
     7021 ggtgacgtaa ctctgaccga accccgcaaa aaaggggtcg gacgccttga ggtacacaga
     7081 gaggctttgt cggtcgtcca cgttttcctt tattgaagtg ataacaaccc cttcgatggg
     7141 gcgcggggcg ttcggcttct gcgaactgcc cgcatgtttc tgagggcaac ccgcataccg
     7201 tgggtcaatt gtttcgtcgg ccatcgttat ttccttccgt ggcacctcag cgggggacaa
     7261 cgcatgttgt tcaagataac gaaatcgccc cgcaacgcga aaacggagtg ctccccgaac
     7321 tgcctggtgg tgccggtatg atccgtccag ggcggttgac gcggaagacg gctgcgctca
     7381 gcgtgggccg cggtgcgggt gtggccgcgc agttcgcgct gaacatgttc ctggttcgcg
     7441 tcgtcagcca gcacgaattc ggctggtttc acgaaataag agtcgtactc acgtttgccg
     7501 cgcttctcga tctgggcatt cctctcggtc tgcttcaggt ggcgggggca gcggaacccg
     7561 gtcgccggca cggtctctac cggaccgccc tggcaatcgc gggcgcagca ggtctggcga
     7621 ccggcgcgat tcttttcctc gcgggtttac tccccgcttt tacggggctg tcctccgcat
     7681 tcgcctggtc gggactcctc atcgccgcca cgatcgttgc cgcggtgctc gaatctgcac
     7741 tcgtggtgga agaacgccac gctgcaatcg ccgcgatttc ggcaacaagc gccgtcgggg
     7801 gactggttct tgcgctggcg ggagttctct ggcggccttc gatcagcgtc gcgtacgccg
     7861 cgctggctct ggcggcctgt gcgcgtgccg tggcgctggc gaaggttacc ggtgtcgcac
     7921 ggaccggtgt tcagtggcgc ccgctggcag gcgcggtcca gctcctgaaa accagcatcg
     7981 cggtgtcagg ccatcgcgca ctcggtatcg cgtcagggac catcgacaga gcagtggtgg
     8041 gtatcttctt ctccgcgggc acccttggct ggtacgtcac gggggcgtgg gaagtgccct
     8101 tcatgtcggt gtttttcagc gcaatcgcgt cggcgatact gccggagatg agcgcgcact
     8161 ggacggcgaa aaagaacgcg gacgtcctcg cgatctggca ccggtcgatc gcactctccg
     8221 cgtggctgat ttttcccatc tggctctggt cgtggatctg ggcaccggaa ctgctccgcg
     8281 ttcttttcac gcggcagtac gaggacggac tcccggtgtt ccgcatctac ctgcttgcgt
     8341 tgcccgtgcg cattgcggtg tactccgccc tgcttgtcgc aatggannnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnntgct tgcgttgccc gtgcgcattg cggtgtactc
     8521 cgccctgctt gtcgcaatgg agaaatcaag ggtgctcgtg aacggggcgg taattgatgt
     8581 ggcagtcaat ctggtgttga gtgtggtact ggccgtggcg attggcccgc tgggccccgc
     8641 agtcgccacc gtggtggcta cctattcgca agcggggtat tatgtcgcca gcgtgcgggg
     8701 agacctgcca gtgcagcggg tcggtttctt cccgtgggga agcctcttct gggctttgac
     8761 ggccgccgga ctcgtaacac tgcctactct cgtcctgcgc cgcgtgttcg agggtgacct
     8821 gccgagggca ggcattgcac tcgcatggac ggcctgcatt ctcgccgccg gagcgttttt
     8881 cgccttgcgc aaaaggggca cggaaggaga ataggccgca cgtgtgcggg aatgcgccgc
     8941 attcagaccg aggtgcttcc cccggcgaac tcaggcagga ggtcgcggcc tttcgcgata
     9001 agtgcggcaa caatcctgcg cgcccagtat tccccctctt cagcggtgct ctccagcgcg
     9061 tcgcgcccac agccgatgaa ctcccccgcg tcgctaagcg cctgaaggtt cacctcgtcc
     9121 ggcaggagcg ccatcaacag ggaggtctcc cacttgcctg cgtggtctcc gtgccccggt
     9181 tcgcctgcct cttccagaat ctggccttcg tgtcccgccc agtgtgcaag gccgagatgg
     9241 cgcgcggcga gatggccgac gaaccgcccg taatgggtca gtggataatg tccgttcaga
     9301 aaaatcagcc ctttgaaccc gagggatttc acctggcagc aaatcttcca tagcagcgcg
     9361 gtataatcct gtgcctgggc aaggatagaa cccgactgcg tataaccggg cttaaaatca
     9421 tcgggatcca aacccatcct cgtaacgatt tcctttccca cgctcgcatt gatctccatc
     9481 aggtgcgact cccggtgttc gccgtaccac accacaggaa acacgattcc tccgcctcct
     9541 tcggccgcgc gaaggcacaa cgcgtgcgct ttcaggccgt ccagccccac cggattgtgc
     9601 ggaccgtgcc actcgagcgt tccgagaggc acgtatgcca gcggaaaagc ctcccgcagc
     9661 cgtatgatat ccgcggggcg gaggtgttcg taccgacgct ccattttgtc tcccctctgg
     9721 cgcgggcctg agtcggcgtc ccggcattgc acgcgaacgc aggacacacc gggccgcatg
     9781 ccgccgcttg acctatttcg tctcgccgcg tagtgtataa ttctacgagt tgcgattgac
     9841 gcgaagaaac ggggccacag tggcgaaacg agttcgagct ccctcctcag ttcccgcgga
     9901 catctcccac gcgccagatg tggtgcgcct tcggggtatg cagttctacg gatttcacgg
     9961 cgcgacccct caggaacgca aaatcggcca gcgattcgaa gtggacgtgg aaatggccat
    10021 tgacgccgaa aaggttgtgg acggcgacct ctccaccacc ccggattacc gcatcgtcta
    10081 cggcatctgc aacgcctgcg tcaccgaacg gcggtaccgg ctgatcgagg tgatggcgcg
    10141 ggaaattgcg cacgagattc tggcaacctt tccgatggtc ggcgtgcggg taacggtgag
    10201 gaaaccgtgc gtgccccttg gaggcgtggt gacggtgggc gccgaagccg tggtgcaacg
    10261 gggcaacctt tgaaccgctc acggtgcttt ctggccctcg gatcgaacct gggtgaccgc
    10321 agggagaatc ttcgccaggc ccttgcgagg gtatcgtgtg gcggcgatct ggagatcgtt
    10381 gcggtctccc gcctctacga aacggttccg gtgggaccgc cccaggggcc ctattacaac
    10441 gcggtggcag aaatcgtgac cgggctcgcg ccggaggaac ttctcgcgcg gtgccggagc
    10501 gcggaaaacg ccggcggccg ccagcggacg gtgcgctggg gaccgcgcac cattgacatt
    10561 gacattctcg actgcggcgg gatggtgcgc gaaaccgcgg aactgcgtct gccgcacccg
    10621 gaaatggcac gccgcgcatt tgtgcttgtt ccgctccgcg atgtcgcgcc ggactgggtg
    10681 catccggtcg aacatcggag cgtcggggaa cttatcgagg cactgggtga taccggcgga
    10741 gcgtgtgtgg taatcgaggg gagtggctgg ttcgtgccac ccctgggaat gcgcggggaa
    10801 ggtgcgtcat catgcggatg attacgactg tcgccgaaat gcggcagtgg tctgaccacg
    10861 agcgccagcg tggcacgcgc atcgggttcg tgccgaccat gggggcgctc cacgccggac
    10921 acctcagcct tgtcgcacgt gcccgcacga aggcggacgt cgtcgcggcc agcattttcg
    10981 tcaaccctac tcagttcggg ccgcgggagg attttgcttc ctacccgcgg acactcgagc
    11041 gagacgttga cctcctcgcg caggagggcg ttgcggtggt gtttgcgcct tccaccgagg
    11101 agatgtaccc ctccggctgc atcaccacgg tgagggtgga gggcctttcg gcgaagctcg
    11161 agggagccat ccggccttcg catttcgccg gcgttaccct ggtcgtcgcc aagctgttcc
    11221 acatcgttca gccccatgtg gcggtattcg gacgaaagga cgcgcaacag gccatcattc
    11281 ttcagcgcat ggtgcgggac ctcgacttcg gcgtggaact cgacgttgcc cccaccgtgc
    11341 gcgaggcgga cggccttgcg atgagctcgc gcaacgtgta tctctcgatt gcggaacgaa
    11401 aacaggcagt cgtgcttgcc cgcgcgctgc agaccggttt ccgcgcatgg agcgaaggcg
    11461 agcggaaaag cgcggtggtg cgggcgctga tgatgaacgt gctggcggaa gaaccggccg
    11521 cgcggctgga ttacctcgag atcgtgtcgc agagaacgct tgagcccgtt gcgctggtgc
    11581 gcagcggaac gcttgtcgcc ggggccatcc gcgtgggaaa aacccgcctg atcgacaact
    11641 ggtgggtccg cgaggacggc gaggcggagt tctaacgccc gccagatcgc ctggtaagcc
    11701 attcgttaca cggaacgcat attcagtccc ctttgaaagg aacgtagtac catggacgcc
    11761 ctcccgacct acgtcaccat gtgccgcgcg aagctccaca acgcgaccgt caccgaaacg
    11821 aagctgcagt acgaaggcag catcacggtg gaccagaacc tcctcgatgc cgccgggatg
    11881 tacctgtacg aaaaagtgca cgtggtgaac ctgaacaacg gcgagcggtt tgaaacctac
    11941 atcattccgg gcgagcggga taccggcgag gtctgcctga acggcccggc cgcgcggctt
    12001 gcggaggtgg gcgacaccgt catcgtcatc gcctacagct tcgtaccgga ggcggatgcg
    12061 cccgcgtttc ggcccacgat tgtggtgctg gggccacaga accagttaag ggaaatactg
    12121 cgccccgcgt aggcgatgaa a
//
LOCUS       DAOTNG010000058        12075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5040_length_12075_cov_5.060732, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000058 DAOTNG010000000
VERSION     DAOTNG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5040_length_12075_cov_5.060732"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>1781
                     /locus_tag="P5179_04300"
     CDS             73..>1781
                     /locus_tag="P5179_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS94474.1"
                     /translation="MKDVTGLPECDFGTLEGYVERLHKSVDKEKLPVYNDELYLELHR
                     ACQTTQARTKRNNRKGELLFRDTEYLSSVAMLAGAEYPQQEIYEQWKPFLTNQFHDIL
                     PGSSVNEVYTQADKDYASILGKLSSLREQALATISRGIDTAGAGTPVVIYNTLGWDRD
                     DVASVAVTGDARVGILDENGKPVVSQVVTERDGQKRLLFEVSGVPSMGHSVYRMVENP
                     REQKSRLKVSESRLENDFFVIQLSKTGTLSRLYDKRNRREVLPKGARANELQLFDDRP
                     FAHDAWDIDFDIDKNRWPMDDVVSAHVTETGPVRATVRIVKKTEKSTLTQDISIWRNI
                     PRVDFVTSVEWWEKRRLLKAAFPVDVLSRTATYEVQFGAIERPTHFSTSYDRAKFEVP
                     GHRWVDLSESGYGVSLLNDCKYGFDIHENVMRISLLRSAIRPDPKADEGHHEFTYSLY
                     PHAGSWRDANTVHRAYELNAPLIARVVPPNKGAKPANESFVRVDRNSVVIDTVKKAED
                     SDAVIIRCYEAHGVRGPVTMSFASPPQQVSECDLMEENDVPVRISGSSFEFSIRPWEI
                     RSF"
     assembly_gap    1782..1881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2047..2472
                     /gene="ndk"
                     /locus_tag="P5179_04305"
     CDS             2047..2472
                     /gene="ndk"
                     /locus_tag="P5179_04305"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRS94475.1"
                     /translation="MDGTLMLVKPDAMQKGALGEILSAVQKEGFTVRNLKMLRLRAAE
                     AKEFYSEHRERPFFNDLVEFMTSGEIAAIALERNDAVATLRKLIGATDSRLAEPGTIR
                     ARFGTDNQMNAVHASDSVSSAQRELAFFFSTRELVPLPD"
     gene            2504..3454
                     /locus_tag="P5179_04310"
     CDS             2504..3454
                     /locus_tag="P5179_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRS94476.1"
                     /translation="MAEQVSTSLDSLGLTRIGGHGSSGAVNRVAVVGGGAMGQGLAQA
                     VSQTGIEVILVERNERTLEAAIEGLSRSLDKEIDKWGLTSSEKRAVLARVIGTTDLGQ
                     TARAPFVIEAIQEKAEDKATLLRDLDAICAEQAVLATSATSLSVTCIAEGVTRRERVI
                     GTHFLDPAPRVPLVEVVRGKHTSDATFVKAKNFIEKQLKKTVVEVADKPGGITTRVLL
                     ALLIEGFRCLTDEVASPEQIDTAVRLGWGFPVGPLEFADQMGLDRVLVWMQNLSREPG
                     GERFHPCPILVDLVNKGHCGKKAGRGFFEYDPEGRKLVAS"
     gene            3579..4349
                     /locus_tag="P5179_04315"
     CDS             3579..4349
                     /locus_tag="P5179_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRS94477.1"
                     /translation="MLVRGKRFLITGVADQNSIAWGITESLMREGAEGLAFLYQPRFE
                     RNVRELTAGIEGALLLEADISAPESLHAAFQTLENHWQDLHGMVHCIAAAKREELRGS
                     FSQTSRDGFLFAHEISAYSLLDLARRAVPLMERNGGGSILCLSYLGGQRVVPNYNVMG
                     VAKASLEMAMRYLAAELGPKSIRVNALSPAPIRTISARVISGVSEMFQRMEERSPMRR
                     NVTRGEVGDAALFFLSDLSRAVTGSVLFVDNGYHIMGA"
     gene            4363..5226
                     /gene="nadC"
                     /locus_tag="P5179_04320"
     CDS             4363..5226
                     /gene="nadC"
                     /locus_tag="P5179_04320"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRS94478.1"
                     /translation="MTEFPQGYVHALVQRALEEDVGTGDITSAATVPESTVARAHLVA
                     KAAGVLSGVDVAEATFLAIDPGIRFSTCAGEGCRVAKGDLVFTVEGRARALLAAERTA
                     LNFLAHLSGVATLTARFVDAVAGSGARILDTRKTTPGMRLLEKRAVRSGGGMNHRVGL
                     YDMVLMKENHIRAAGGITKAISACNQYLRHQGLTGTKVEVETTCEEEVREALAAGCDR
                     IMLDNMTEDEMRSSVLLIRSIAPATEIEASGNMTLERVPAVAKTGVDFISIGALTHSA
                     PVLDLSLQFEM"
     gene            5274..6083
                     /locus_tag="P5179_04325"
     CDS             5274..6083
                     /locus_tag="P5179_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRS94479.1"
                     /translation="MTRALGRTHFAFGSVTTTMELASRLAKAGAPHGSIVTAEQQTAG
                     RGRRGRKWISEPGLGIWCSVLLRTQECAGPAAPALGLIAACSVVAAARMNGVLCAGVK
                     WPNDVCALEHNAGAHCGESPVRWPSAWVLSARKIAGILVETSKELSGCFVVGIGVNVH
                     HSRSDFPSEFASSASSVDLCAGRDVARPALLADVLCCFEMRLGWSEAMVRSEWHRESL
                     TIGRRVKVSGPDGSFIGQAEAIEADGALKVSGPSGTVTVTGGETTVRCVSD"
     gene            6191..7087
                     /locus_tag="P5179_04330"
     CDS             6191..7087
                     /locus_tag="P5179_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94480.1"
                     /translation="MEMLRVSVRNNAVSLRKGAWLTRTLEESDPRVRVQVSPLAQEEH
                     DTEAGESLLWTALRTDAADCALADASTLTTWPPPPWVRAVAFLARMNPRFALLHRSSV
                     KLSDLPAAPRLSTSSGLARVQFDNAKPGAEWVELSGDLPTRLHKLHLGHADGVIEPAS
                     DLLTLGIAEDAIEFLSTDMLLPAPGQGVWIVFSLSDRADVERVFRRLDDRKAGICAKA
                     ERTFTAQLDAPANCLVGARAWVQTKRLTLDACIMPAEGGVVQRLSISGAPEQPEELVA
                     GLVDMFRHQSVLRQCRGKADRG"
     gene            7090..7548
                     /gene="aroQ"
                     /locus_tag="P5179_04335"
     CDS             7090..7548
                     /gene="aroQ"
                     /locus_tag="P5179_04335"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRS94481.1"
                     /translation="MRILVLNGPNLNMLGIREPAVYGSQSLAGVEAELASLASQLGVE
                     VECHQSNHEGALIDMIHAAFDRFQGILINPGALTHYSYALRDAITSVGLPAVEVHLSN
                     IHAREPFRHQSVIAAVCVGQISGFGVESYQLGLRALVSYLQKTQERIRKA"
     gene            7611..7684
                     /locus_tag="P5179_04340"
     tRNA            7611..7684
                     /locus_tag="P5179_04340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7645..7647,aa:Arg,seq:tct)
     gene            complement(7743..8570)
                     /locus_tag="P5179_04345"
     CDS             complement(7743..8570)
                     /locus_tag="P5179_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS94482.1"
                     /translation="MIKSINLWSFRDSNDLKTNVPLAKKAGFEAIELCLNEDTGPITW
                     KSTEREVKEVRKFVEGAGLKIASLAIGTGWKYPLSSPDKKIVKAGADSIKKGLDVAKW
                     LGTDGVLVVPGVTTPEVPYDVVYKKTQDELKKLVPYAEKAKVAICVENVWNKFHLSPI
                     ELRDYVDSFNSEFLAVYLDVGNMLLYGYPEQWIRILGKRIKKVHFKDFRTGVGGFGGF
                     VGLLEGDVDWPAVMKAFKEVGYDGPASVEVGCYKHHWEASIYVIGLQMDFIFGRRSG"
     gene            complement(8554..8991)
                     /gene="acpS"
                     /locus_tag="P5179_04350"
     CDS             complement(8554..8991)
                     /gene="acpS"
                     /locus_tag="P5179_04350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRS94483.1"
                     /translation="MSRIFGIGVDVVDVARIDRAANRWGDRFLKRVYTQRELEYCRER
                     PLPAEHLAARFAAKEATMKALGTGWTPHVAFKAIEIVREGRRRPQIELNRDMRRMLPA
                     NLTLHVSLSHTRLLATAFVVAALESEPSLNSSSTREKHHDQIH"
     gene            complement(9025..9579)
                     /locus_tag="P5179_04355"
     CDS             complement(9025..9579)
                     /locus_tag="P5179_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94484.1"
                     /translation="MTTAFVVTGAIVLIAAVAVVVWFFTGRRPPRLDPRSERLIESLV
                     QLQRDNPRRATEVFARALVRAGLVWEKDLQTGGTSAQTDRQQPYRDSRRDQPQRQHPS
                     RNEQRVEVRAPAQQAAQRPEVRDTRTGERTESSAASGDKTGSPPQPATHSSRRRRRRR
                     PSGTREQQPGATPQQTTTPPPDQA"
     gene            complement(9873..12026)
                     /locus_tag="P5179_04360"
     CDS             complement(9873..12026)
                     /locus_tag="P5179_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HRS94485.1"
                     /translation="MGKKHKDLRHWLNRGALTVLILVFCVSVSVAQNIGKDGGSGSAT
                     DERRELRIGDRTLGTISTSGGSIRYGLNVNATKTLTIRMDKVLDSAIDPKLTIYDSNG
                     SQVATDDDGGEGLNALISVTLQPGQYVVAAQAVGTSSGDFLLRVEEQQATTLSLGTAV
                     LGEITTSMNEVAYRVNIPRTTNLRIRMNKLLGSTLDPKVTLVDGSGTEIATDDDGGGS
                     LNALLQRSVPSGEYVVRARRVGTSTGKFMLSVYDQDAPLGGASVRVGDSVTEIINDPN
                     VPAVFRLTVNDEFQDCRITVDKTEGSNLDPNLIVTNRSGEIIGSDDDSGEGNGALLRL
                     SLGRGDYQLFVGGYGTTTGAFRLTVAPDRVPSISVGETKRGVLATGGSDLYRFTTPRA
                     REVTISLRKAAAPATSQSVQQFRQIGSIPSGGGFSRFAYTVDSEQPLVVRVDKIGDGS
                     LDPKVTVYDQNGNEVGSDDDSGDGLNARLSRVFAPGVYTIQVSSVNNSSGDFILMVGP
                     DTGGGSTLDPKLIVKNPSGSEVASDDDGGEGNDALLTTNLAAGTYQIIAMAYGSTSGA
                     YELTVSEVVPQDMGAISPGQTKTGTLTSGGRHRYSLRVASSAVIAIDVMKAADSSLDP
                     QVVLRGSSGEEIAMNDDGGDGNNARIIRRLEPGSYEIVVRGYQATAGGYTLMVESFAD
                     TRTPAPQREQMEDSPSKGGREGSGSEPEGFGKGAG"
BASE COUNT         2459 a         3367 c         3625 g         2524 t
ORIGIN      
        1 ttgtctatca cgtttggtca cggtgacgga gggggcggcg ttaccatcga aatgttcgag
       61 aatgttctca gaatgaaaga tgttacgggt cttccggaat gtgatttcgg cactcttgag
      121 ggatacgtcg aacggcttca caaatccgta gacaaagaaa agctccctgt gtacaacgac
      181 gagttgtacc tggaactgca ccgtgcctgc cagaccactc aggcgcgaac aaagaggaac
      241 aatcgaaagg gagaactgct attccgtgat acggaatatc tttcgagtgt tgccatgctc
      301 gcaggcgctg aataccctca acaggagatc tacgagcagt ggaaaccgtt cctcacaaat
      361 cagtttcacg acattctgcc agggtcttcg gtgaacgaag tatacactca ggccgacaaa
      421 gactacgcgt ccattctggg caaactgagc agtttgaggg aacaggccct cgcgacgata
      481 agccgcggca tcgatacggc gggtgcaggg acaccggtag tcatctacaa caccctcggc
      541 tgggacaggg acgacgtcgc cagtgtcgcg gtaactggtg atgcccgtgt cggaattctc
      601 gacgagaacg ggaagcccgt cgtaagccag gttgtcaccg aaagagacgg tcagaaaagg
      661 ctgttgtttg aggtgtccgg cgttccatcg atgggccact ccgtctatcg gatggtcgag
      721 aacccgcggg agcagaaatc caggctgaaa gtctcggaat cgcgtctgga gaatgatttc
      781 ttcgtcattc agctttccaa gacggggacg ctgagtcgct tgtacgacaa acggaacagg
      841 cgcgaggtac tcccgaaagg ggcacgcgca aacgaactgc agctgttcga tgatcgacca
      901 tttgcgcacg acgcctggga catagatttc gatatagaca agaaccggtg gcccatggac
      961 gacgtcgttt cggcgcacgt gacggagacg gggccggtca gagcaaccgt tcgcatcgtc
     1021 aagaagactg agaaatcgac gttgacacag gatatttcca tctggaggaa catcccgagg
     1081 gttgacttcg taacttcggt cgagtggtgg gagaagcggc gccttctgaa ggctgcgttc
     1141 ccggtcgatg tgttgtcacg tacggcgacg tacgaggtgc agttcggggc gatagaacgg
     1201 cccacccatt tctcgacctc ctacgaccgc gcgaaattcg aggttccggg tcaccgctgg
     1261 gtagatcttt ccgaatcggg gtacggcgtc agtctactga acgattgcaa atacggtttt
     1321 gacatccacg agaatgtcat gcggatcagc ctcttgcggt cagcgatacg ccctgatccg
     1381 aaggcggacg aaggacatca tgagtttacc tacagcttgt atccgcatgc cggttcgtgg
     1441 cgagatgcga atacggtgca ccgggcgtac gaactgaacg cgccactcat cgcgcgcgtg
     1501 gtgccgccga acaaaggcgc aaaaccggcg aacgagagct ttgtgcgcgt agaccggaac
     1561 agcgtggtga tagacacggt gaagaaggca gaagactcgg acgcggtgat catacgttgc
     1621 tatgaagcgc acggcgtccg ggggcctgtt accatgtcgt tcgcgagtcc gccgcaacag
     1681 gtcagcgaat gtgatctgat ggaggaaaat gacgttccgg tgaggatttc cggttcgtcc
     1741 ttcgagttct caatcagacc atgggaaatc cgctcattca annnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn naggtcggag tgtcgtgcgt gcttgttgac cggctcggcc
     1921 gaccccgata ccttgaatca ttctggctcg agaaacattt ccagtcgttt ttcgcgcgga
     1981 accgccccga acccgactgg tgcccagcgc cgtagcggga atcaagggga aacacaggag
     2041 aaagacatgg acggcacgct gatgcttgtc aaacccgacg cgatgcagaa aggggccttg
     2101 ggcgagatcc tctccgcagt gcagaaggag ggtttcacgg tccgcaatct caagatgctc
     2161 cgcctccggg ctgcggaagc gaaggagttc tattcggagc accgcgaacg cccgttcttc
     2221 aacgatctgg tggagttcat gacgagcggc gaaatagccg ccatcgcttt ggaacggaat
     2281 gatgctgtcg ccacgttgag gaagttgatc ggggcgacgg attcccggct tgcggaaccc
     2341 ggaactatac gcgcgcgttt cgggaccgac aaccagatga atgcggtgca cgcgagcgac
     2401 agcgtcagca gtgcacagcg ggaacttgcg tttttctttt cgacacggga actagtcccg
     2461 ttgcctgatt gacaaagtta ttctccagac aggagcgtat tccgtggcgg aacaggtgtc
     2521 cacctcgctg gattccctcg ggttgactcg tatcgggggg catggcagtt cgggtgcggt
     2581 taatcgcgtg gccgtggttg gtggcggagc aatgggccag ggcttggctc aggccgtttc
     2641 ccagaccggg atcgaggtga ttctggtaga acgcaatgaa cgaaccttgg aagcggcaat
     2701 tgaaggtctt tccaggagcc tcgacaaaga gattgacaag tggggcctta cgagttcaga
     2761 aaagcgggcg gttcttgccc gcgtgatcgg aacgacagac ctcggacaaa ccgcacgggc
     2821 tccgtttgtt attgaagcta ttcaggaaaa ggcggaagac aaagcaacgc tcctgcgcga
     2881 tctggacgcc atttgcgctg aacaggcggt gctcgccaca agtgcgacgt cgttgagtgt
     2941 cacctgtatc gcggaggggg tgacgaggag ggaacgggtg atcggtactc acttccttga
     3001 ccccgctccc cgggtgcctc tggtggaagt cgtgcgcggc aaacacacct cggacgcgac
     3061 gtttgtgaag gcaaagaact tcattgaaaa gcaactgaag aaaacggtcg tcgaggtcgc
     3121 cgacaagccg ggaggaatca cgacgcgcgt tctgcttgcg ttgctgatag aagggttccg
     3181 ctgtctcacc gacgaagtcg cttccccgga acaaatcgat accgcggttc gcctgggttg
     3241 gggtttcccc gttggccctc tcgaattcgc cgatcagatg gggctggacc gggttctcgt
     3301 gtggatgcag aatctgtcgc gggaacccgg cggcgagcga ttccacccgt gcccgattct
     3361 tgtagatctt gtcaacaaag ggcactgcgg aaaaaaagca gggcgcgggt ttttcgagta
     3421 cgatccggaa ggcagaaagc tggtcgccag ctagcggtgg cactggtttc tgcgggagta
     3481 gcggaccgcg gcaattcctt tcgagccgcg cgtgccggta cgtggtttgg tcggatctgg
     3541 acagcacggc gcaacgtgga attcgggagc gtgaggatat gctcgtacgc gggaaacggt
     3601 tcctcatcac aggcgtggcg gaccagaaca gtattgcatg gggcataacg gaaagcctca
     3661 tgcgtgaagg agcggaaggc cttgcgttct tgtatcaacc gcgatttgag aggaacgtcc
     3721 gggagctcac cgcgggaata gagggtgctc tgctgctgga ggccgatatt tctgctccag
     3781 aatcgctgca tgctgccttt cagactctcg aaaaccactg gcaggacctt cacgggatgg
     3841 tacactgcat agccgcggcg aagcgagagg aactgcgggg gagtttttct cagacttcgc
     3901 gcgatgggtt ccttttcgcg catgagatca gcgcatattc tcttcttgat ctggcaagaa
     3961 gagcagttcc gttgatggaa aggaacggcg gcgggtcgat actctgcctt tcgtacctcg
     4021 gaggccagcg ggtcgttccg aattacaacg tgatgggcgt tgcgaaggct tcacttgaaa
     4081 tggcgatgcg gtaccttgcc gccgaactcg ggccaaaatc aatccgggtc aacgcactca
     4141 gcccggcgcc catacgtact atttctgccc gcgttatctc aggagtctcg gagatgttcc
     4201 agagaatgga agaacggtct cccatgcgca ggaacgtgac gagaggcgag gtcggtgacg
     4261 cggcgctttt cttcctgagt gatctctccc gggcggttac cggttcggtt ctctttgtcg
     4321 acaacggcta tcacataatg ggcgcatgat gaggcaacgc cagtgacgga atttccgcag
     4381 gggtacgttc acgcgctggt tcaacgcgct ctggaagagg atgtcgggac aggcgatatc
     4441 acgagtgccg cgaccgtgcc tgaatcgacg gtggcgcggg cacacctggt ggccaaagcc
     4501 gccggcgtgc tttctggagt tgacgttgcc gaagcgacct tcctagcgat agaccccgga
     4561 atcaggttct ccacctgcgc aggtgaaggt tgccgggtgg cgaagggcga cctggtgttc
     4621 acggtggaag gccgcgcccg ggctctgctt gccgcagaac gaaccgcgct gaatttcctg
     4681 gcgcacctgt caggcgtcgc aaccctcacc gctcgctttg tggatgcggt ggcggggtcc
     4741 ggagcccgta tactggacac tcgcaaaacc actcccggca tgcgcttgct ggagaaacgc
     4801 gcggtaagat ctgggggcgg aatgaatcac cgcgttggtc tgtacgacat ggttctcatg
     4861 aaagagaatc atatccgtgc ggcgggtgga ataacgaaag cgatctctgc gtgcaaccag
     4921 tatcttcgac accaggggct gaccggaaca aaagtcgaag tggagacgac gtgcgaggag
     4981 gaggtccgcg aagcgctggc agcgggttgt gaccgaatca tgctggacaa catgaccgaa
     5041 gatgagatgc gctcgtcggt gctcttgatc cggtcgatcg cgcccgcgac cgagatcgag
     5101 gcttcgggca acatgacgct ggaacgggtt cccgccgtcg cgaaaacggg cgtagacttc
     5161 atttcaatag gtgcgctcac acacagcgcc ccagtccttg acctctccct tcaatttgag
     5221 atgtgaagag aagtcctgat ctcataacgc cggacgccct gagcggctgc atcgtgacac
     5281 gggccctcgg tcgaacgcac ttcgcgttcg gctctgtaac caccacgatg gagcttgcct
     5341 ctcgtcttgc gaaggcggga gcgccccacg gttcgatcgt tacggcagag cagcagacag
     5401 cgggacgggg taggcgggga cgaaaatgga tttcggaacc ggggctcgga atctggtgtt
     5461 cagtgctgct gaggacccag gagtgcgcgg ggccggccgc tccggcgctg gggctgattg
     5521 cggcatgttc ggtggtggcg gccgcaagaa tgaacggtgt actgtgcgcg ggtgtcaaat
     5581 ggccgaatga cgtgtgcgcg ttggaacaca acgcgggggc acattgcggc gagtcacccg
     5641 tcagatggcc gagcgcgtgg gtgctttccg cgaggaagat agccggcata cttgtcgaaa
     5701 catcgaagga actgagcggt tgtttcgtcg tcggaatagg ggtgaacgtc caccattcgc
     5761 ggtcggactt cccatcggag tttgcgtctt cagcatcgtc ggttgatctt tgtgcgggac
     5821 gtgatgttgc acggccggcc ctacttgctg atgttctttg ttgtttcgag atgagactgg
     5881 gctggtccga agcgatggtg cgttcggaat ggcatcggga atccctgacg atcggcaggc
     5941 gagtcaaagt atccggcccg gatgggagct tcatcggaca ggctgaagca atcgaggcag
     6001 atggtgcttt gaaggtgagc ggtccttcag gaacagtcac ggtaaccggc ggagagacca
     6061 cggtgagatg cgtttccgac tgaccgacgc gatgctaccg ccgttcagtt cgtaaggtcc
     6121 caggtcgttc gggggattcc aaacggggaa tgcgttggaa gacattcagg gtggagaaac
     6181 gaaacccgtg atggagatgc tccgtgtctc ggttcgaaac aacgctgtct cgctccgcaa
     6241 aggtgcgtgg ctgacgcgaa cactcgaaga atctgacccg cgagtgcggg tacaagtgtc
     6301 tccgctggcc caggaggaac acgacaccga ggcgggggaa agcctcttgt ggacggcgtt
     6361 gcggacggat gcggcggatt gcgcgcttgc ggacgcaagt acgttgacaa catggccgcc
     6421 gccaccctgg gtgcgcgcag ttgccttctt ggcgcggatg aacccgcgtt ttgcgctctt
     6481 gcaccggtca tccgtcaaac tttccgatct gccggctgca ccgcggttgt ccacgtccag
     6541 cggtcttgcg agggtgcaat tcgacaacgc gaaaccggga gcggaatggg tagagctttc
     6601 gggagacctt ccgacgcgtc tgcacaagtt gcatctcggg catgcagatg gagtcataga
     6661 acccgcgagc gatctgttga ctcttggcat cgcggaggat gcaatcgagt tcctgtccac
     6721 tgatatgctt cttccggcgc cggggcaggg tgtgtggatc gtcttctcgc tctccgacag
     6781 agccgacgtg gaacgggtgt tccggcgcct tgacgaccgg aaagcgggga tctgcgcgaa
     6841 agccgaacgc acgttcaccg cgcaactgga tgctccggcc aattgtcttg ttggcgcgcg
     6901 agcgtgggta caaacgaagc gcctgacgct cgacgcatgc attatgccgg cagaaggggg
     6961 tgttgttcag cgcctgagta tctccggggc gcccgaacag ccagaagagc tggttgcggg
     7021 gctggtcgat atgttccgcc accagagtgt cctgcgtcag tgtcgcggaa aggcagacag
     7081 ggggtgagca tgcggattct tgtgctgaac ggcccgaacc tgaacatgct cggcattcgc
     7141 gaaccggcag tgtacggcag ccaatctctt gccggcgttg aggcggaact tgcttcgttg
     7201 gcatctcaac ttggcgttga ggtggagtgt catcaaagca accacgaagg ggctctgatt
     7261 gacatgatcc acgccgcgtt cgatcgcttt cagggcatcc tgatcaaccc gggcgccttg
     7321 acgcattaca gttacgcgct gcgcgatgcg atcacctccg tcgggcttcc tgcagtggag
     7381 gttcaccttt cgaacatcca tgctcgcgag ccttttcgtc atcagtcggt gattgcggca
     7441 gtatgtgtcg gtcagatctc ggggttcggg gttgaaagct accaactcgg attgagagcg
     7501 ttggtgtcgt acttgcagaa gactcaggaa aggatccgca aagcgtgagc caccgcatcg
     7561 gctggtgccg tttctgcgga tgagtacatt tcagatatcc ccgactttct ccccggatag
     7621 ctcaacagga tagagcaagt gccttctaag cactaggtcg caggttcgag tcctgctccg
     7681 gggataccca aaaaaagccc gcggactggt gtccgcgggc tgcgttattt ctgggaatcg
     7741 ggttacccag aacgccgtcc gaaaatgaaa tccatctgga gaccgattac gtaaatagaa
     7801 gcctcccagt ggtgcttgta acaaccgact tccacagaag cggggccatc atagcctacc
     7861 tctttgaagg ccttcatcac ggcgggccag tcgacgtcgc cttcgaggag gccgacgaat
     7921 cctccaaacc cgcctacgcc cgtgcggaag tccttgaaat gaaccttctt gatccgcttt
     7981 ccgagaattc ggatccactg ctccgggtag ccgtacagca acatgttccc gacgtccagg
     8041 taaacggcca gaaactcgct gttgaagctg tccacataat cccggagctc tatcggactc
     8101 aaatggaact tgttccacac gttttcaaca cagattgcga ccttcgcctt ctccgcgtac
     8161 ggaaccagtt tcttcagttc atcctgagtc tttttgtaaa cgacatcgta cgggacttcc
     8221 ggagtagtca cgcccggaac cacaagcaca ccgtctgttc ccagccactt cgcgacgtca
     8281 aggcctttct tgatcgaatc ggctcccgct ttcacgatct tcttgtccgg agatgacaga
     8341 gggtatttcc aacccgtgcc aatggccagc gaagcaattt tcaatccggc cccctcaacg
     8401 aacttccgga cctctttgac ctccctctcc gtgctcttcc aggtgatcgg accggtgtct
     8461 tcattgaggc acagctcgat agcttcgaac cccgctttct tcgccagcgg tacgttggtc
     8521 ttcaggtcgt tggaatcccg aaacgaccac aggttaatgg atttgatcat ggtgcttctc
     8581 ccgggttgag gacgaattga gactgggttc tgattccaga gccgccacca caaacgcggt
     8641 ggcgagaaga cgcgtatgag aaagcgatac gtgcaaggta aggtttgccg gcaacatccg
     8701 tctcatatcg cggttcagtt caatctgcgg tcgccggcgg ccttctctga cgatttcgat
     8761 ggccttgaag gccacatggg gtgtccaacc ggtgccaagt gccttcatgg tcgcttcttt
     8821 tgcagcaaac cgggccgcca ggtgctccgc cggaagcggc cgttctcggc aatactccag
     8881 ctcgcgttgc gtgtacacac gcttcagaaa gcgatccccc catcggttgg cggcacggtc
     8941 gatacgtgca acatcgacga cgtcgacgcc gatgccaaaa atcctgctca cgacagcctc
     9001 aacaatcctg cacttcgcgt tctatcacgc ctgatccggc ggcggcgtag tcgtttgttg
     9061 cggggttgca ccgggttgtt gctctcttgt ccccgatgga cgacggcgtc tgcggcggcg
     9121 cgatgaatgc gttgcaggct gtggcggact gcccgttttg tctccgctcg ccgccgatga
     9181 ttcggttctt tcaccggttc ttgtgtcgcg aacctccggc cgctgcgctg cctgttgcgc
     9241 gggagcacgg acctcaacac gctgttcgtt gcgggaaggg tgttgccgct gaggttggtc
     9301 gcgtcgcgag tctcgatagg gttgttgacg atcagtctgc gcagaagtcc cccctgtttg
     9361 caggtccttc tcccacacca gaccggcacg gaccagcgcc cgggcgaata cttccgtcgc
     9421 acgccgggga ttatcccgct gaagttggac aagcgattcg atgaggcgtt cgcttcgcgg
     9481 gtcaaggcgg ggaggacgcc ggccggtgaa aaaccagaca accaccgcga ccgccgcgat
     9541 cagaacgatc gccccggtta ccacaaaagc tgtagtcatt caccacctcg ggttttcgtt
     9601 accatacgtg ttcctcaatt cggaagtcgt tctgagttcg gcgcaccgga acccggcggc
     9661 gtccagaaaa ggcgtgcaag ccagaactcc tgtggaggac tggctatctc cagggtacgg
     9721 acggattgcc aaaggcaaag gcttcgaaat ggatgcacaa tcagaaagta cggcaatacg
     9781 aacctcccct caagcgcgga ggttcacccg cacaactgaa tacaaagaaa aaggggcgct
     9841 tttgagcgcc ccgcttcttc cgggttcacc tatcagcccg cgcccttacc gaaaccttcc
     9901 ggttcgcttc cgcttccctc gcgtccacct ttggagggag agtcttccat ctgctcacgt
     9961 tgcggggcgg gtgtgcgtgt gtcggcaaac gattcaacca tcagggtgta tccacccgct
    10021 gttgcctggt atccgcgcac gacgatttcg tacgatcccg gttccaatcg gcgaatgatg
    10081 cgggcattgt tgccgtcacc accgtcgtca ttcatcgcga tctcttcccc ggacgacccc
    10141 cgaagaacta cctgaggatc cagcgaggaa tcggcagcct tcatcacatc gatcgcaatg
    10201 acggcggatg aggctacccg aagggagtac cggtgccttc cccctgaagt cagagtccct
    10261 gtttttgtct gtcccggtga aatcgcgccc atgtcctgcg gcactacttc cgaaaccgtc
    10321 aattcatatg caccggaagt cgatccatag gccatcgcga tgatctggta cgttcccgcc
    10381 gccaggttcg tggtgagtag agcgtcattg ccctcgccac cgtcatcgtc tgaagcgact
    10441 tcacttcccg aggggttctt gacgatcagt ttcgggtcga gcgtgctgcc tccaccggtg
    10501 tccggcccta ccatcagtat gaaatcgccg ctgctgttgt tcacggagct gacctggatg
    10561 gtgtacacgc cgggagcaaa cactcgcgaa aggcgtgcgt tcaggccgtc gccgctgtcg
    10621 tcatcagaac ccacctcgtt tccgttctgg tcgtacacgg tcaccttcgg gtccagcgaa
    10681 ccgtccccaa ttttgtctac acgtacgacg agcggctgct ccgaatccac ggtgtacgca
    10741 aacctgctga aacccccgcc ggacgggatc gatccgatct gccggaattg ctgcacactt
    10801 tggctggtcg ccggtgccgc agcctttcga agtgagatgg ttacctcacg ggcgcgcggt
    10861 gtggtgaacc tgtatagatc actgcctccc gtcgccagaa cgccccgctt cgtttctccg
    10921 accgagattg agggaacccg gtcaggagcc accgtgagac ggaaggcgcc ggtggttgtt
    10981 ccgtaaccgc cgacaaacag ctggtaatca cctcgcccga gggacaatcg caacaatgcg
    11041 ccgttgccct ctccagagtc gtcgtcgctt ccgatgatct ccccgctgcg gttcgtcacg
    11101 atcagattcg gatcgaggtt gctcccttcg gttttgtcca ccgtgatacg acaatcctgg
    11161 aactcgtcgt tcacggtcag acggaaaacc gctggcacat tcgggtcatt gatgatctcc
    11221 gtgacggaat caccgacacg cacactggct ccacccagag gagcatcctg atcatagacc
    11281 gagagcatga atttcccggt gctggtgccc actctgcggg cccgaacgac gtactcaccg
    11341 ctcggaaccg acctctggag gagggcattc agacttccac ctccgtcatc atccgttgcg
    11401 atttccgtcc cgctgccatc aacaagcgtc actttgggat cgagtgtaga accgagcagt
    11461 ttgttcatcc ggatacgcag attcgtggtt cgcgggatgt tcacgcggta ggcaacctcg
    11521 ttcatgctgg tcgtgatttc gccaagcact gcggttccga gagacagggt ggtagcctgc
    11581 tgttcctcaa cacggagaag aaaatctccg cttgatgttc ccactgcctg agctgccacc
    11641 acgtactgtc cgggttgcag cgtgaccgag atcagcgcgt tcagcccctc cccaccgtcg
    11701 tcgtcagtcg cgacttgcga accattggag tcgtaaatgg tgagttttgg gtcgattgcg
    11761 gaatccagga ccttgtccat ccgaatcgtc agggtttttg tggcgttcac gttgagtccg
    11821 taacggatgg aacccccgct tgtcgaaatc gtacccagcg tcctgtctcc aatccgcagc
    11881 tcgcgccttt catccgtggc ggaacccgag ccgccgtcct ttccgatgtt ctgcgcgacg
    11941 gaaactgaaa cacagaacac cagaatcagc acggtcaacg ccccccggtt cagccagtga
    12001 cgcaagtctt tgtgtttctt tcccatttcg ttcccctccc gattgtgtct tggcggtgtg
    12061 aaacctggta tgatc
//
LOCUS       DAOTNG010000059        11918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5114_length_11918_cov_5.130068, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000059 DAOTNG010000000
VERSION     DAOTNG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_11918_cov_5.130068"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1548
                     /locus_tag="P5179_04365"
     CDS             <1..>1548
                     /locus_tag="P5179_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94486.1"
                     /translation="PWEIWAAMQEAYLAAFPDIAGIRSSMGDFSEQYQIWRAHGPRSA
                     AIGPAGGMRRFLEYARDAVVRKHGRELIVSAWGNPPEEYPLNQPAALRRIFADVSPED
                     PISLLSNECEHDFYLNSPFNDNFAAIDIRRGIMFQVQREYQGQGDLPVYVGRRIREHL
                     GRAAGYGATDMAAARLWWSKNLYNEGVCWTWWNMYAWQRATWEPNGDPWEWARDYSTI
                     AFGRQGARELADALMLTEELAARLFTIPGFTGGKRDAYTLTHRNVITDGRHYWRRTPD
                     PQGEGFREHHLGGRLTELLVSVDATERLADTLFAKARVARAAMGDSEKARAVMSSFEH
                     LHTLAPVLTHYQRAILLWHGKDAAQLTPAMREEARGRCVKECYATLAAFGEYRARYDM
                     YRDGGMVPLVRSWLRALGEPLPPEIEVPPQFPIARSARPLPVDGTLRGWPAPAIVMDT
                     SNAEVFGNAGDFRAEIRLAWDADALKVAVDAPGGWPAEGIRAGRLYYSDSVSLSFDTD
                     NDGREDA"
     assembly_gap    1549..1648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1649..2049
                     /locus_tag="P5179_04370"
     CDS             <1649..2049
                     /locus_tag="P5179_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94487.1"
                     /translation="DSVSLSFDTDNDGREDAIYFLLIDGASGEPRLHTLMRRMVFYSS
                     NYEERGAEPPPDPGASVKLVKTDSGRRWVAAIPISMLDGYRPDSGVPLGVRVQVNVNS
                     APGLGAHRVAWPRNPAWRDAPPEWMGYGVR"
     gene            complement(2527..3414)
                     /gene="thyX"
                     /locus_tag="P5179_04375"
     CDS             complement(2527..3414)
                     /gene="thyX"
                     /locus_tag="P5179_04375"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRS94488.1"
                     /translation="MADTRRFSNPAADAWIDKPIPCLDHGFVMLKDYMGGDASIVQAA
                     RVSYGDGTKKASDDKGLIAYLMRHRHTTPFEMVDLKFLVKLPIFVARQWIRHRTASVN
                     ELSARYSVMADEFYLPKKEDVRYQSTLNRQGGSDEEVSDELCGRVVETLRAGAERAYN
                     EYEEMIEANIARELARTHLPVSLYTQWYWKINLHNLLHFLELRLDAHAQYEIRVYAQA
                     MSQIVKDVVPWTWEAFEEFRLNAQTFSASEKAVLAMLLQGKEVVLPESLQKGGRRREF
                     EEKLAALGVDPATVLPPAK"
     gene            complement(3629..4579)
                     /locus_tag="P5179_04380"
     CDS             complement(3629..4579)
                     /locus_tag="P5179_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS94489.1"
                     /translation="MQVTIWGCRGSLPTPMPKAEYEKKLSDILTAYRDAGAPADIEAF
                     LQSRPFVERSTFGGNTACVEVTEGDHQLILDAGSGLRPLGLKMMGGPCGKAKGDVHML
                     ISHTHWDHIMGFPFFVPAYIPGNKITIYGCHDNLKERFEYQQVFTHFPVLLDQMASTK
                     TFVQVEVGKPFEIGPFKISTIAHFHPGDAYGYRIETCKASFVYATDAEYTEVTWEQQQ
                     KHIEFFRGAGAIVFDAAYSLKEAVEKLNWGHGSPLLGVEMAVQAGAKRLILFHHDPAT
                     PEKQIAEAHAKAKRFYDELPNKTTECEILTAHDYMTLDLG"
     gene            4816..5175
                     /locus_tag="P5179_04385"
     CDS             4816..5175
                     /locus_tag="P5179_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRS94490.1"
                     /translation="MSATVRITRGVVVITPDQKRLTAHRGMPAVRTRLEEAVAQGKKA
                     VAVDFSGVEYVDSAMMGVLIDAFKGIHRNGGRLCVFGAGDAVHEVLKNTVLDDVIEIV
                     PDEDAAIEALKGKKRNL"
     gene            5222..5995
                     /locus_tag="P5179_04390"
     CDS             5222..5995
                     /locus_tag="P5179_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRS94491.1"
                     /translation="MSVHVKEVNGVYVVHPRFARLTATHGAREFRAALEKGVRDGYEG
                     IAIDFSGVKFADREILHVLAAAYKLHSPSYGLMFAFHCSDDLEEFFEETLLDRLIPVV
                     KDEEAALAVLAGCRKRPVKTRAAEKVLGKAYAVGHRIGALPDRRVAAVNVPPEHERRR
                     GARAAAETTGRPDVEETPVTVQPEKAPQRVEEVTPEPLKEPAAPTKQPSRAKRPAKAQ
                     KETEERVLQPGAAEAPKKPKAPARKKTPRAPRKKPEQGG"
     gene            complement(5992..7107)
                     /locus_tag="P5179_04395"
     CDS             complement(5992..7107)
                     /locus_tag="P5179_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS94492.1"
                     /translation="MELFRTAFLVLLALGATGYAVASVALWTGALRRSRRIPRGGDLP
                     TVTVIVPARNEAANIASCLTALSRQSYPPHLCKIVVVDDRSDDGTGEIAARAACAMEA
                     VVQVVRVDTVPPGWSPKKHALHRAILESTSDIILTTDADCEPEECWIEEMVASLSAAH
                     VVAGYSPYRDRTSLPARLLALETLSQAFLAMGGIGIGRPITCTGRSLGYRREVYAAVG
                     GFGEAHSMLSGDDHLFLQRAVGGGFTAAFCDGPGSYVWTNPPPTWRGFVQQRIRMFSG
                     ARMLKPAVAALGVFVYAWLAVMGVGMLAAFPAAWGAFGVKVLLDGVSMGIAAVRLHEK
                     RLLAVYPIAAFLYLPYFLVFALWGTVGSYRWKGARGH"
     gene            complement(7412..7867)
                     /gene="bcp"
                     /locus_tag="P5179_04400"
     CDS             complement(7412..7867)
                     /gene="bcp"
                     /locus_tag="P5179_04400"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRS94493.1"
                     /translation="MPTKGDNAPDFSLADQNGVVHTLKQYAGKWVVLYFYPKDDTPGC
                     TKEACSFRDNLGAVTKKGAVILGVSGDSTASHKKFATKYDLPFPLLADTEKAVINAYG
                     VWKEKNMYGKKTMGVERSTFLIDPAGKIAAEWRKVKVDGHTEEVLAALP"
     gene            complement(7971..8633)
                     /gene="trmB"
                     /locus_tag="P5179_04405"
     CDS             complement(7971..8633)
                     /gene="trmB"
                     /locus_tag="P5179_04405"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRS94494.1"
                     /translation="MARISIRSIRYRRPLNPTWDTELEQQIHCQIEGIEAPLDLAGLF
                     QRQAPVHVEIGFGKGRFLLAAAERFPETNWLGIEYAPPCVALAVERAARKGLRNVRIA
                     RGVAEDIIAARFPADTVAAYHIYFPDPWPKKRHHKRRLIKPPFAVQLARTLEPGGFVN
                     TATDNSEYFEEIIAACTGAGLARMDVPDIYGDEAFRTNFEMRFLTHGQPVYRAAFRKR
                     DR"
     gene            complement(8656..9705)
                     /locus_tag="P5179_04410"
     CDS             complement(8656..9705)
                     /locus_tag="P5179_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS94495.1"
                     /translation="MQPVQVSSPVAGDVGRNRLARRILNNALASIIEPVRPHFDSVLR
                     QLDSILTAEVPTVDAVSRYVAATKGKCIRPALVLLAARCVGAITHEVRLAAVSMELLQ
                     ASTLLHDDVIDQSVLRRGVPSVNARWDNETAVLMGDILFTQALSTMLETGSIPVMKIA
                     ALQTRRMIEGEVHARDQRRSPVFSEESYLDLINRKTASLMSLATEGGARLSDGTEQQV
                     NALASYGELLGMAFQIVDDILDVVGDPTVVGKPTGQDLREGTITLPLIRALNAAPHED
                     AQKIRELVSLGVNTDADWNAVHAFIELYGGVASALRTARHYAEQARAHLGILPPSDAR
                     DSLDRMVGYVISREI"
     gene            9908..11041
                     /locus_tag="P5179_04415"
     CDS             9908..11041
                     /locus_tag="P5179_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS94496.1"
                     /translation="MKYRRFGKTELMVSVFTFGAMRIPDGTEENAVATIRRAVELGIN
                     HLETARGYGSSERLMGLAMPSLPREKLIITTKIGPADTADEMRRSLDESLERMRVDRI
                     DNFDIHGINNREILRRALKKGGCLAAIRKAMEEGIVGHLGFSTHAPLEVIMEAIGTGE
                     FESVNLHYYTFNRRNLPAIEKARSLDMGVFIISPTDKGGQLFNAPAKLRELTKPYTPI
                     QANHRFLLGNPEIHTLSLGAASPHEFEEHLAVADNDGPWTEEEAALFARLDEEMRRAL
                     GTTFCTQCYECLPCPENIHIPETLRLRNLARAYDMKEFGKYRYNLFSRGGHWFPGEQA
                     TACTKCGECLPRCPERLDIPALLMDTHELLLGDPQGHLYARND"
     gene            complement(11177..>11918)
                     /locus_tag="P5179_04420"
     CDS             complement(11177..>11918)
                     /locus_tag="P5179_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRS94497.1"
                     /translation="PDKRIGKLVPVDEITPNPGQPRRHFDPDALEALKESIRQHGLIN
                     PITVDENGRILAGERRYRACVALGMREVPVIRMSGSLEVSLVENLQRQDLHPLEEARG
                     YQTLVDAGYTHEQIATRIGKDRSVVTHSLRLLDLPADIQAECVTSHNVTKDQLLQILG
                     ADSPEQQRRIWEDIKAGKSARALKQERSEAKRRRQITPRVFINRVRSFSTDAGKIDVA
                     RLSDTEKRALKTHIEHALARLSSLLGEL"
BASE COUNT         2264 a         3345 c         3987 g         2222 t
ORIGIN      
        1 gccgtgggag atatgggcgg caatgcagga ggcgtatctc gcggcgtttc cggatatcgc
       61 cggtatccgg tcgtccatgg gcgatttcag cgagcagtac cagatctggc gggcacatgg
      121 gccccggtcg gcggcaatcg gcccggcggg gggcatgcgg aggtttttgg agtatgcccg
      181 ggatgcggtg gttcgaaaac acgggcggga actgatcgtg tcggcgtggg gcaacccgcc
      241 ggaggaatac ccgttgaacc agccggcggc gttgaggagg attttcgcgg acgtatcgcc
      301 cgaggacccg atttcgctgc tttcgaacga gtgcgagcac gacttctatc tgaattcccc
      361 gttcaacgac aacttcgccg cgatcgacat ccgacgcggg atcatgtttc aggtgcagcg
      421 cgaatatcag gggcaaggtg acctgccggt gtacgtgggg cgacgcatcc gtgaacacct
      481 cggcagggcg gctggctacg gcgcgaccga catggccgcc gcgcggctct ggtggagcaa
      541 gaacctctac aacgagggtg tgtgctggac atggtggaac atgtacgcct ggcaacgggc
      601 aacatgggag ccaaatggcg acccgtggga gtgggcgcgg gattactcca ccatcgcgtt
      661 cgggaggcag ggcgcgcgcg aacttgcgga tgcgttgatg cttaccgaag aattggcggc
      721 gcggttgttc acgattcccg gatttaccgg cggcaaacgg gatgcctaca cgctgaccca
      781 ccggaacgtc atcacggacg ggcggcacta ctggcgccgg acgcccgatc cgcagggaga
      841 ggggttccgc gagcatcatc tgggtggacg tttgacggaa cttcttgtat cggttgacgc
      901 gacggagcgg ctggcggata cgctgttcgc aaaagcgcgc gttgccaggg cggccatggg
      961 ggatagcgaa aaggcacggg cagtgatgag cagtttcgaa cacctgcaca cgctggcccc
     1021 ggttctcacc cactaccagc gggcgattct gctgtggcac gggaaggacg cggcgcagtt
     1081 gacgccggcg atgcgggaag aagcccgggg ccgctgcgtg aaggaatgtt acgccacgct
     1141 ggccgcattc ggcgaatacc gcgcgcggta cgacatgtac cgggatggcg gcatggtgcc
     1201 gctggtgcgg tcatggttgc gcgcgctggg ggagccgctg ccgccggaga ttgaggtgcc
     1261 gccgcagttc ccaatcgccc gctctgcgcg cccgcttccc gtggacggaa ccctgcgcgg
     1321 ctggccggcg ccggcgatcg tgatggatac gagcaacgcc gaagtgttcg gcaacgcggg
     1381 agatttccgg gctgaaatcc gcctggcgtg ggatgccgat gcgctcaagg ttgccgtgga
     1441 tgcgccgggc ggctggccgg cggagggaat acgcgcgggc aggctctact acagcgactc
     1501 ggtatcgctc agcttcgaca cggacaacga tggccgggag gacgcgatnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gactcggtat cgctcagctt cgacacggac
     1681 aacgatggcc gggaggacgc gatttacttc ctgctcatcg acggcgcgtc gggcgagccg
     1741 cgcctccaca cgctcatgcg gcggatggtg ttctactcgt cgaactatga ggaacgcggg
     1801 gccgaaccgc cgcccgaccc cggagcgagc gtgaagctgg tgaaaacgga ctccggcagg
     1861 aggtgggtgg ccgccattcc gatttcgatg ctggatggct accgcccgga ctcgggggtg
     1921 ccgcttggcg tgcgggtgca ggtcaatgtc aattcggcac cggggttggg cgcacaccgg
     1981 gttgcctggc cgcggaaccc tgcgtggcgg gatgcaccgc cggagtggat gggatacggg
     2041 gttcgctgac gcgacccctg gtgagcgcgg cgtgggcggc gggagcaggt cctatggggc
     2101 gtataggacc tgtgacgccg ttgcctgtcc ctccaccgcg agcgaccagg acacgccggc
     2161 gcgtctcgat acgtcgtctg cggtgcggtg caaaaaagga cacgacggag cgtgtccctc
     2221 catcgggtgt gggcatgggt cgcaggtggt aacaactttt tggggtccgc accatggatt
     2281 cccgcctgcg cgggaatgac ggcttcggga gatcgctgca actgcagatt gttgcgctcg
     2341 aagtgattga atcgcgggat ccgtccgaag gcgctggaac cggagcgcaa aggacacgac
     2401 ggagcgtgtc cctccatcag gtgttacggg cggctcctcg atacaacacg gcgtcgcgga
     2461 tctccatcgt gtcactcgga gcgcgcgccg cgccgccagt cgacgaccgg agtgaaccga
     2521 gtgcctctac ttcgcaggag gcaacaccgt tgccgggtcc acgccaaggg cggcgagctt
     2581 ttcctcgaat tcccgccgtc ggccgccttt ctggagggat tcgggcaaca ccacctcctt
     2641 gccttgcagc agcatggcaa gcaccgcttt ttccgaggcg gagaaggtct gcgcgttgag
     2701 gcggaactcc tcaaaggctt cccatgtcca cgggacgacg tccttcacga tttgcgacat
     2761 tgcctgcgcg tagacacgga tttcgtactg ggcgtgggca tcgaggcgga gttcgaggaa
     2821 gtgcaacagg ttgtgcaggt tgattttcca gtaccactgg gtgtaaagcg atacgggcag
     2881 gtgagtgcgg gcgagttcgc gggcgatgtt ggcttcgatc atctcctcgt actcgttgta
     2941 ggcacgttcc gcgccggcgc ggagggtttc caccacccgg ccgcagagct catctgaaac
     3001 ctcctcgtcg ctgcctccct gccggttcag ggttgactgg tagcgcacat cctccttttt
     3061 cggcaggtaa aactcgtccg ccatgacgct gtaccgcgcg gagagttcgt tgacactggc
     3121 cgtacggtga cggatccact gccgggcgac gaaaatgggc agtttgacga ggaacttcag
     3181 gtccaccatc tcgaacggcg tggtgtggcg gtggcgcatg aggtaggcga taaggccctt
     3241 gtcatcggag gcctttttcg tgccgtcgcc gtaactgacg cgggctgcct ggacgattga
     3301 ggcgtcgcct cccatgtagt ccttgagcat aacgaagccg tggtcgaggc aggggatggg
     3361 tttgtcaatc caggcatcgg ctgcaggatt ggaaaaacga cgagtatctg ccatacaagt
     3421 tctctccgcg cagaagatga gaaatggaag caagggaaag acaacgtagg gaatgcgaag
     3481 gaagcggtca agacggacgc aaaaagaagc ggggagcacc ggactttgct ccccgcctga
     3541 aagcagcctc acgcggtcgt gagcaggctc acccgaggtc gagcgggctc acgcaggggt
     3601 gagcgacctc acccgaggtc gagcgacctc aaccgaggtc gagggtcatg taatcgtgcg
     3661 cggtgaggat ttcgcattcc gtggttttgt tgggcagttc gtcgtagaac cgttttgctt
     3721 tggcatgtgc ttcggcgatc tgtttttccg gggttgccgg gtcgtggtgg aacaggatga
     3781 ggcgcttggc gccggcctgc accgccattt cgacgccaag gagcggggat ccgtggcccc
     3841 agttgagctt ttcgaccgcc tctttcaggc tgtatgcggc gtcgaacacg atggcgcccg
     3901 cacctctgaa gaactcgatg tgtttctgtt gctgttccca cgtgacttcg gtgtactcgg
     3961 cgtccgtggc gtacacgaaa gacgctttgc aggtttcgat gcggtacccg taggcgtcgc
     4021 cggggtggaa atgggcgatt gtggagatct tgaacggccc gatctcaaac ggcttcccga
     4081 cctctacctg cacgaacgtt ttggttgagg ccatctgatc gagcaaaacg gggaaatggg
     4141 tgaacacctg ctggtactcg aaccgttcct tgagattatc gtggcagccg tagatggtga
     4201 ttttgttccc cgggatgtag gccggcacga agaaagggaa gcccatgatg tgatcccagt
     4261 gggtgtggct gatgagcata tgcacatcgc cctttgcctt cccacagggg ccacccatca
     4321 ttttgaggcc gaggggtcgg aggccgctgc ctgcgtccag aatgagctgg tggtcgcctt
     4381 cagtaacttc gacgcaggcg gtgttcccgc cgaacgtgga acgctccacg aacgggcggc
     4441 tctggaggaa tgcctcgatg tcggcggggg ctccggcgtc ccgatacgcg gtgaggatgt
     4501 cgctgagttt cttttcgtat tccgcttttg gcatgggtgt cggcaatgat ccccggcatc
     4561 cccagatggt gacttgcatg gctggaatcc cccgcgatca ggtggtgttt ctgccgacgg
     4621 catgcgaaat tctgtctggg caagaaaata ggaaaaccag cgccggaaag caaaccgcaa
     4681 ccgtaaccgc ccgtgtggtt ttgtgttagg gtcgccgcga acgggattcc ttgacccacg
     4741 gttccgctgc atctaagttg aatggttgtc tgcaccaaac aggcctttgt ctgttccgta
     4801 tgaaggggtc gagccatgtc tgccaccgtc cggatcacac gcggggtagt agtcatcaca
     4861 ccggatcaaa agcgtcttac cgcgcaccgg gggatgccgg ccgtccgcac acgtcttgag
     4921 gaggcggtag cgcagggaaa gaaggccgtt gccgtggatt tttcgggtgt ggaatatgtg
     4981 gacagcgcca tgatgggggt tctgattgac gcgttcaagg ggatccatcg aaatgggggt
     5041 cgtctgtgcg tgttcggcgc gggtgatgcg gtccacgagg ttctgaaaaa caccgtgctg
     5101 gatgacgtaa tagagatcgt accagatgag gacgcagcga ttgaggcttt gaaagggaaa
     5161 aagcgaaacc tgtgaagccg ggctgacgag tgtcggcagg aatctttcgg ggggcaggaa
     5221 catgagtgtg catgtgaaag aggtgaacgg cgtgtatgtc gttcatccgc gatttgcgag
     5281 gctgacggcc acacacgggg cgcgggagtt cagggctgcg cttgagaaag gcgttcgcga
     5341 cggttatgag gggatcgcga ttgacttttc gggggtgaag ttcgcggaca gggaaatcct
     5401 gcatgtgctg gccgcggcgt acaaacttca ctcgccctcg tacgggctga tgttcgcgtt
     5461 ccactgcagc gatgatctgg aagagttttt cgaagagacg ctactcgacc ggctgattcc
     5521 cgttgtgaaa gatgaagaag ccgcgctggc cgttctcgcc gggtgcagaa agcggccggt
     5581 gaagacgcgg gcggcggaaa aagtgctcgg gaaggcatac gcggtggggc accggatagg
     5641 ggcattgcct gaccggcggg ttgctgcggt gaacgtacct cccgagcacg aacgccgacg
     5701 aggagcccgg gcagcggcag aaacaacggg taggcccgat gtggaagaaa ccccggtcac
     5761 cgtacaaccc gaaaaggcgc cgcagcgggt ggaggaagtg acacctgaac ctctgaagga
     5821 acccgccgcg ccaacgaaac aaccgtcgag agcaaagcgg cctgcgaagg cgcaaaaaga
     5881 aacggaagaa cgggttctgc agccgggggc tgcggaggca ccgaagaagc cgaaagcgcc
     5941 cgccagaaag aaaactccgc gtgcgccgcg gaagaagccg gaacaggggg gttagtgccc
     6001 gcgggcacct ttccatcggt atgagccgac cgtaccccac agggcaaata cgaggaaata
     6061 gggcaggtag agaaacgcgg caatcgggta caccgccagc agccttttct cgtgcagacg
     6121 aactgcggca attcccatac tgacgccgtc gagcagcact ttcaccccga acgcgcccca
     6181 tgctgccgga aaggcggcaa gcattccgac acccatcact gccagccacg catacacgaa
     6241 aactcccagc gctgcgacgg caggcttcaa catgcgcgcg cccgagaaca tgcggattcg
     6301 ctgctggacg aacccgcgcc acgtcggcgg cggattcgtc cagacgtagc tgcccgggcc
     6361 atcgcagaac gcggccgtga aaccgccccc gaccgcgcgt tgcaggaaga ggtggtcgtc
     6421 gccgctgagc atggaatggg cttcgccgaa cccccccacg gcggcgtaga cctcgcggcg
     6481 gtagccgagg cttctgccgg tgcaggtgat gggtctgccg attccgatgc cgcccatggc
     6541 gaggaaggcc tgggagagtg tttccagagc gagcaaacgg gccggaaggg aggtgcggtc
     6601 cctgtacggg gagtagcctg cgaccacatg tgccgcggaa agactggcga ccatttcctc
     6661 gatccagcac tcttccggct cgcagtcggc gtcagtggta aggatgatgt cggaggtgct
     6721 ttcgagaatg gcccggtgca gagcgtgctt tttgggcgac caacccggcg gcacggtatc
     6781 cactctgacg acctgcacga cagcctccat cgcgcaggcc gccctcgccg cgatttcgcc
     6841 ggtgccgtcg tcggagcggt catccacgac aacgatcttg cagaggtgag ggggatagga
     6901 ctggcgtgag agagcggtca ggcacgaggc gatgttcgcg gcctcgttcc gcgccggcac
     6961 gatgacggtg acggttggca ggtcgccacc acgagggatg cgccgagacc ggcgaagcgc
     7021 gccagtccac agcgcgacgc tggcaacggc atagcccgtt gcaccgaggg cgaggagaac
     7081 aaggaacgcg gtccggaaga gttccatggg cgccgccgtg agagattcgg ggaacaccgg
     7141 gtctgtgtga aaatttagga cacgggaaaa acggcgggaa aggggggagc gggggccccg
     7201 cggacgtggt catctcgata caacgttcgg ggaacggcgc ttcgcttcgt tctccagaag
     7261 tcactcgatg accgtgcgcg cgcaaaaacg gaaaaaaggg ggggcgggca acccgccccc
     7321 tacgaggccg ggcctatgaa gaatccgggc aggcagggaa accgcccctc agacctcaat
     7381 tcaggtgcgt gggaggatcc ctggctcgcg gttacggcag ggcggcaagg acctcttccg
     7441 tgtggccgtc aaccttcacc ttgcgccact ccgcggcgat tttgccggcg gggtcaatga
     7501 ggaaggtgga gcgctcgacg cccatggtct tcttgccgta catgttcttc tctttccaga
     7561 cgccgtacgc gttgataacc gctttttccg tgtcggcgag aagcgggaag ggaaggtcgt
     7621 atttcgtggc gaatttcttg tgcgacgccg tggaatcgcc gctgacgccg aggatcactg
     7681 cgcccttttt cgtgacggcg cccaggttat cgcggaagct gcacgcttct ttcgtgcaac
     7741 cgggcgtgtc gtctttcggg tagaagtaga gcacgaccca tttgccggcg tactgcttca
     7801 acgtgtgtac taccccgttc tgatcagcga gactgaagtc cggtgcgttg tccccttttg
     7861 tcggcatggc tgttcctccc ttatgcaaga cgtgtacggg atcgttcgtc gaaggtacaa
     7921 aacgcaggga acgaggagaa gattccgccg aattgggaga agagaacagg ttaccggtcc
     7981 cgtttgcgaa acgcggcacg gtagaccggt tggccgtggg tgaggaatcg catctcgaag
     8041 tttgtgcgga aggcttcgtc cccgtagatg tccggcacgt ccatgcgtgc aagtccggca
     8101 ccggtgcacg cagcgatgat ttcctcgaaa tactcgctgt tgtctgttgc cgtattgaca
     8161 aaccctccgg gctccagcgt tcgggcgagt tggacggcaa acggaggttt gatgaggcgc
     8221 cgcttatgat ggcgcttttt tggccacggg tccgggaagt agatatggta ggcggcaacg
     8281 gtgtccgcgg ggaagcgcgc agcaatgatg tcctccgcca caccgcgggc gattcggacg
     8341 ttacggaggc cttttctggc tgcgcgttcc accgcaagcg cgacgcacgg cggggcgtat
     8401 tcgatcccca gccagttcgt ttcggggaat cgttctgccg cggcgagcag gaaccggccc
     8461 ttgccgaaac cgatttcgac gtgcacgggc gcctgccgct gaaagagccc ggccaggtcc
     8521 agcggggctt cgatgccctc gatttggcag tgaatctgct gctcgagttc ggtatcccac
     8581 gtgggattga gcggacggcg gtaacgaatt gaacggatgg aaatccgtgc catggggacc
     8641 tcagaacggg agcttctata tctcgcggct tatgacatac cccaccatcc ggtcgaggga
     8701 atcgcgagca tcggatggcg ggaggatgcc gaggtgcgcg cgcgcctgtt ccgcgtagtg
     8761 ccgggcggtg cgaagcgccg aggcgacgcc tccgtacaac tcaatgaagg cgtggactgc
     8821 gttccagtcg gcatccgtgt tcaccccgag ggaaacgagc tctcggattt tctgtgcatc
     8881 ctcgtgcggg gcggcattca atgcacggat gagcggcagg gtgatggttc cttcgcggag
     8941 gtcctgccct gtgggtttgc ctacgacggt gggatcgccc acgacgtcga ggatgtcatc
     9001 cacgatctgg aaggccatgc cgaggagctc cccgtacgat gcgagcgcgt tcacctgttg
     9061 ttcggtgccg tcagaaagac gcgcgccccc ctcagtggcc aacgacatga gagacgcggt
     9121 tttccgattg atcaggtcga ggtagctctc ctcggaaaag accggagagc gtctctggtc
     9181 gcgggcgtgg acctctccct cgatcattct tcgggtctga agcgccgcga ttttcatcac
     9241 ggggatcgaa ccggtttcga gcatggtgga aagcgcctgc gtgaacagga tgtcgcccat
     9301 caggacggcg gtttcgttat cccagcgggc attcaccgaa ggcacaccgc gccgcaggac
     9361 cgactgatcg atgacgtcat catgcaacag agtggaggcc tgaaggagct ccatgctgac
     9421 cgcggcgagc cggacttcat gcgtaattgc ccccacgcac ctggctgcca gcagcaccag
     9481 cgcgggtctg atgcatttgc ccttcgtggc ggcgacgtag cgacttacgg cgtcgacggt
     9541 cgggacttcc gcagtaagaa ttgagtccag ttgacggaga acggagtcga aatggggacg
     9601 caccggttcg attattgacg cgagggcgtt gttgagaatg cgccgcgcga ggcggttgcg
     9661 accgacatcg cccgcgactg gcgacgacac ctgtaccggc tgcatacttc ggtttctccc
     9721 gtcccacgga caggaactgg gttcgtgggc ccgtatggtg cgagtgcaag acgggcacgc
     9781 gctcgaaatt gattgatacc gcgtcaccgg cactttggtc aagtccacac gatgacgtca
     9841 tttcataaca caaacatcaa cggggcaggt gtgtgttccg gcggagccca acaggcgagg
     9901 tgacgtgatg aaataccggc gattcgggaa aacggagctc atggtatcgg tattcacgtt
     9961 cggcgcgatg cgtatccccg acggcaccga ggagaatgcg gttgcaacga tccgccgcgc
    10021 ggtggagctg gggatcaacc acctcgaaac ggccagaggg tacgggtcga gtgagcgctt
    10081 gatgggtctt gcgatgccct cattgccgcg cgaaaagctt atcatcacga ccaaaatagg
    10141 ccccgccgat acggctgacg agatgcgccg gtcgctggac gaatcgctcg aacggatgcg
    10201 cgtggatcga atcgacaatt tcgatatcca cggcatcaac aatcgcgaga ttcttcggcg
    10261 cgcgttgaag aagggcggct gcctcgcggc aatccgaaag gcgatggagg aagggatcgt
    10321 cgggcacctc ggtttttcga cacacgcgcc gctggaagtg atcatggagg caatcggaac
    10381 gggggagttc gagtcggtca acctgcacta ctacaccttc aaccggcgca atctcccggc
    10441 aatcgagaag gctcggtcgc tggatatggg cgtgttcatt atctctccga cggacaaggg
    10501 agggcagctt ttcaacgctc cggcgaaact ccgggagttg acaaagccct acacgccaat
    10561 ccaggccaat caccggttcc tgctgggcaa tccggagatt cacacgctga gcctcggggc
    10621 ggcctctccg cacgagttcg aagagcatct tgccgttgcg gacaacgatg ggccgtggac
    10681 cgaggaagaa gccgcgctgt tcgcccggct ggacgaggaa atgaggcgag cactcgggac
    10741 cacgttctgc acccagtgct acgagtgtct gccctgcccg gagaatattc atattccgga
    10801 gacgctgcgg ttgcgcaacc tcgccagagc gtacgacatg aaggagttcg ggaaataccg
    10861 ctacaacctg ttcagtcggg gcggacactg gtttccggga gaacaggcaa ccgcctgcac
    10921 gaaatgcggc gagtgcctgc cccggtgccc ggaaaggctt gatatcccgg cgttgctcat
    10981 ggacacgcac gagcttctgc tgggggaccc tcaggggcac ctgtacgcac gcaacgatta
    11041 gaactcggct cgcgcgggcg ggccgagttc ttttggagtc ccctgaatcg cgcttcaggg
    11101 aactccaggt cagcgtggcg gcgcagtcaa cgggttttct gcgcgagggg gtgggctcga
    11161 cagggagggg tgggcctcag agctcgccaa gaagggacga aagacgcgca agggcgtgtt
    11221 cgatgtgcgt tttcagggca cgcttctcgg tgtcgctcag ccgggcgaca tcgatttttc
    11281 ccgcgtcggt gctgaagctt cttacgcggt tgatgaacac acggggcgtg atctgccggc
    11341 ggcgttttgc ctcactgcgt tcctgcttga gagcccgggc ggacttcccc gctttgatgt
    11401 cctcccatat tcttctctgc tgctccgggc tatccgcgcc aagtatctgt aataattgat
    11461 ccttagtaac gttgtgtgac gtcacacatt cggcctgaat atccgccggg aggtcgagca
    11521 ggcgaaggga gtgggtgacc accgacctgt ccttcccgat gcgcgtggcg atctgctcgt
    11581 gggtgtaccc cgcgtccacc agagtctggt atcctcgcgc ttcctcgagc gggtgaagat
    11641 cttgccgctg caggttttcc accagggaga cttccagcga gccgctcatg cggatgaccg
    11701 gcacctcgcg catacccagt gcgacacacg cccggtagcg acgctccccg gcaagtatcc
    11761 tgccgttttc gtccacggtg atcgggttga tcagcccgtg ctggcgtata ctttccttca
    11821 acgcttcgag tgcgtccgga tcgaaatgcc tgcggggctg gccgggattc ggcgtgattt
    11881 cgtctacagg caccagtttg ccgatacgtt tgtcgggc
//
LOCUS       DAOTNG010000060        11883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5130_length_11883_cov_5.198766, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000060 DAOTNG010000000
VERSION     DAOTNG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_11883_cov_5.198766"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="P5179_04425"
     CDS             complement(<1..1197)
                     /locus_tag="P5179_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS94498.1"
                     /translation="MVRIVFLLVCATCVLPAAPGYAQPLEERVVEHTLDNGMKFLFVK
                     RGTAPVFSGVVIVRVGGVDDPQGQSGLAHLFEHMAFKGTPRIGTVDFAAEQGIMRHID
                     SVAVLYTQTLATVPLAHRGALEALERRTAREVVVANQGSEDYEITRALANALAETLRT
                     APDRYPSLRAYARAKGYLADLQRYTAIHRTFIVKDEFSRIIDRNGGVDNNAGTGKDYT
                     IYYESFPANRLELWALLESERFMNPVMREFYSERDVVAEERRMRTDDDPEGKLYEQFM
                     ATAMQAHPYRIPIVGWMSEVEKVTAQDAAAFREAYYVPSNTVGVIVGNISVDSAKAFV
                     GRYFGRIPSTNRRAPEVRTVEPPQAGERRVSVDFDAEPQLMVGYHKPNYPDPDAYVFS
                     VIANILR"
     gene            complement(1212..2381)
                     /gene="rlmN"
                     /locus_tag="P5179_04430"
     CDS             complement(1212..2381)
                     /gene="rlmN"
                     /locus_tag="P5179_04430"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRS94499.1"
                     /translation="MLSTIPEESFDRASALAHPAVCAEKPDLKGFLPDELREFLARFG
                     KEKYRTNQLLRWMHGKGVADFDQMTDLGKEFRSQLKAEARVECLAERHRAVSRDGQTT
                     KLVLGLSDGRKIETVMMLDDGRRTVCVSSQAGCALGCRFCATGQIGFQRNLTVGEILD
                     QIHHAARFFRAWGATGEEYEHPVTNVVFMGMGEPLNNYEAVLQATKLSGLEMGLAISA
                     TRVTISTAGVVPMIYRLASDLPKVGLAISLNATTDEVRNSLMPINRKYPLKTLLDAAR
                     HMAGVSPRRRVTFEYALIEGTNDSDEDARRLAELVSGFPCKINLIPFNPVPRQPFRRP
                     SQERIDRFHRILWERNLTVTVRWSKGDDIAAACGQLQASVPYRSLHGNPSGTETP"
     gene            2515..3279
                     /locus_tag="P5179_04435"
     CDS             2515..3279
                     /locus_tag="P5179_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94500.1"
                     /translation="MVYRFLLAVVATSVICVFVQACTPSRSLPQISTREVPDPFVRST
                     QDASDDSLVRAKWMCRTTVPDPLAGQEDGPKIRFEATLYGEDVIVAEVVKECRGPAGL
                     VASDTCVSASRERYRSVHRDDTRLRIRLDLESSFSVNSLNSRFWDVYLKDERGIAYEP
                     VEIVQNAPVIVREDTLPEPGRAPVRGAVYRRAIDMYFPREDAFGTDVISSSTREVRLV
                     VSQNRRELTTFVWRIKRTGDSGTTRTGSAQRAPSGF"
     gene            3303..3698
                     /locus_tag="P5179_04440"
     CDS             3303..3698
                     /locus_tag="P5179_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94501.1"
                     /translation="MNTHEELVEKVETLAERDGRFRKEAYFFVFEALQFTVKSLNLTG
                     KQRHVTGQQLAHGIKEYALDRFGPMTLSVFEHWGVRSTLDFGEIVFGLVEAGLMGKTE
                     EDSIEDFVDVYDFQKAFDWHNAIGRKLRK"
     gene            3695..4504
                     /locus_tag="P5179_04445"
     CDS             3695..4504
                     /locus_tag="P5179_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS94502.1"
                     /translation="MTSEQPQHTGKPFVLASCSILPEKWNKEGNAEKMLSFMERSLRC
                     GADVIAVPEGCLEGYVVMEAIEGNRGPELLNIAEPEDGEMIGRFRSFCQKHHVHALIC
                     FCERERNEAFNTALWIGRNGETVGKYRKTHLNEGYNPDHPFNSTGNVIRAFDTEFGRA
                     GVMICFDRRVPEVARCLALDGAQFILNPSYGVYKGWNDSVLVARAHENQVPVVFVHPN
                     KTLAVNEKGDITFCREETDVISHLTVTPRTSSSLLLSLRRPELYGRLTSLR"
     gene            4650..6575
                     /locus_tag="P5179_04450"
     CDS             4650..6575
                     /locus_tag="P5179_04450"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRS94503.1"
                     /translation="MAGKSICHRIIEQHLVAGKVIPGTEIGIRIDQTLTQDATGTMAY
                     LQFEAMGIPRTRAGLSVSYVDHNMLQDGFENADDHRYLQSVARKFGVYFSRPGNGICH
                     QVHRERFGVPGQTLLGSDSHTPTGGGIGMLAMGAGGLDVAVAMAGHPYFFPCPRVTLV
                     RLSGRLRPWVGGKDIILELLRRLSVKGGVGRVMEYGGPGVATLPVADRFTVANMGAEL
                     GATTSVFPSDELTRKYLEAQGRPEAWIPMYADPDATYEAVVEIDLDKLEPMIARPHSP
                     DNVAPVRDLEGTPVQQVCLGSCTNSSYHDLRIAAAMLRGRTISPAVSLTVTPGSRQVL
                     EMIARDGSLADLIAAGARVLEAACGPCIGNGQAPPSGANSVRSFNRNFEGRSGTRDAG
                     VFLASPETCVACAITGVITDPRDLGIEFPEIPEPESYSRNDSMVIPPSTEPEEVTVSR
                     GPNIVSLGSFEAMADTLRGRVLIKVGDNITTDHIMPAGRWLKYRSNVPKYSEATFNLI
                     DPDFPKRSREWGGGLIVAGENYGQGSSREHAALVPRWMGVKAVIAKSFARIHEANLVN
                     FGILPLVFADPDEYGRIQQGDTIEIPHARQAVENGLNVIEATNLTQGAKFGLRVNLTE
                     RGRKVVLAGGMLNFVRS"
     gene            6585..7460
                     /locus_tag="P5179_04455"
     CDS             6585..7460
                     /locus_tag="P5179_04455"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRS94504.1"
                     /translation="MENSPSENQLLVGAHMSTEGGLFRAVERGAQIGCTAIQLFTRNP
                     SQWFSPPLSEKDVTEFRDAVSRTKMAAVVAHDSYLINLASPDASLRKRSIAAMLDELD
                     RASALGIPLLVTHLGAHTGQGEENGLQTLADSLNEVIDARPDAPVSVCLETTAGQGTS
                     LGWAFEQIAWVLSKLSAPARFRTCFDTCHVFAAGYDLATRDTYEKTWREFDKVVGIRY
                     LAVMHLNDAKKELGSRVDRHAHIGKGEIGEGAFRRIMQDPELRGVPKILETPESETMH
                     AVNLSLLRELAARTG"
     gene            7508..8347
                     /locus_tag="P5179_04460"
     CDS             7508..8347
                     /locus_tag="P5179_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94505.1"
                     /translation="MDGASKGMTHLGRGIVLALFAVCIGFGGCGRRGSGESDNTDLSK
                     QLVGTWFAYRDNVIENMWTFREDGTCVNDGWPGDVQAPPYHLEGTYSVETAKITVIFK
                     LEEGLTDTVILSDPDITNDRLVYSAGGIPVAFLRERTAAAAESAPTADEGAPVPALAD
                     SIVGGWVAFVGGIPANTWQFNQDGTFVNEGWTQLSPGSVILKRAYQVSGKFAVTGSRV
                     LLTNERIVHFGTGPQGENSEVAVSQNIVLYDVTVSRGRLVYTNEGGLPVAFRRGYVTP
                     TEW"
     gene            8453..11539
                     /gene="glnE"
                     /locus_tag="P5179_04465"
     CDS             8453..11539
                     /gene="glnE"
                     /locus_tag="P5179_04465"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HRS94506.1"
                     /translation="MPDPGKTLQNVLLGVFPELSPDSCTELFVRHFPQHQERVSRAIE
                     TLVGLLPSQTVRLLVETCVVSPDPGAAISCLSELVQAAPSSFRDVAPEQARRLASSLS
                     GGAFLRERILRHATRVFWLVDESGRTFSRPELEREIAETREGSAGWDALRVCHDLHLM
                     RIAVADCAGTLGIVEITEQLSLLAEVTINEVAQRQAQALRARYGDPLQQDGANAEWCV
                     IGLGKLGGNELNFRSDVDVMFVYSAEGYSTGGTSGAVPVAQWYTRWAEGILSVLTEVS
                     STGLLYRVDTRLRPDGQSGALVRSIASYVHYYEQRGEVWERQMLVKARPVAGSVRMGQ
                     KLLDFLESFIYPRSLVTSPREEIHRVKNRILQHLDARATSSNPANAERNLKLGRGGLR
                     DIEFVVQCLQLVVGGSDKAVREQNTLAAIRRLHQRGVLSDTEQRALDEAYRLFRRTEH
                     RLQMATGQPSFVLPSEHDELSLLARRMGYSDPAALMADLETARKNVMSVYDSVLGPPE
                     TGTEIGKILNLPAGDAQAVSELAGCGFRNPEAAHRNLLHLAFGHGDTVPASTPSQPVL
                     RLVPRLLDELRGSANPDRGLNNVERMLRAFGAVDSFADLLSSHNAFLNLLVTIAARSE
                     ALTETISRDPGLVDWMLYSGALFAERHPEDVDLESRAAVAGLPSAALQIAALHSFRKR
                     ENLRVGVRFLMDLANEEETSEQLTAIAESVVKRVVSVASRSLAGAAAENRSYQWAIIG
                     LGKLGGREMNFGSDLDIFFVHAEEQDEGQCVDYVTSLAQEVLRLLCENTEYGVLYEVD
                     TRLRPEGKHGPLSLSIDGFREYLRKRVAQWERQALTFSRVIVSAPQPRFGEVVEEAIW
                     NAIAVKPTREIFESALSMRLRMQEQAQSNYGTRPNIKTGFGGLVDAEFIAQLGRMIGG
                     VRLSEGRKGDTRATLECMRSAGLLPDRVAQTLMDAYHALRRIQMHLRVNDYRAHNVLP
                     DDEDAILTLARGLGFRDRSDLSVHVEKVTEDMRGAFLLALESLATANTKALGGSLV"
     gene            11532..>11883
                     /locus_tag="P5179_04470"
     CDS             11532..>11883
                     /locus_tag="P5179_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94507.1"
                     /translation="MSDRFMGGWRVLAILIAAVISLATGPALSAAEETRNLRIGPIAE
                     VRVSPQFSVSLVIQRRGRSLQELGKHFLARPQHASRYTHRDQMTVPFPALSDPYKAIV
                     ALRLFPEDSLSQDGL"
BASE COUNT         2514 a         3256 c         3569 g         2544 t
ORIGIN      
        1 gcggaggatg ttggcgatta cggaaaaaac atatgcatcc gggtccgggt aattcggctt
       61 gtggtatccc accatcagct gcggttccgc atcgaagtcc accgagaccc ttcgctctcc
      121 ggcttgaggt ggttctacag tccgcacctc aggcgcccgg cgatttgtcg aaggtattcg
      181 tccaaaatac ctgcctacga aagcctttgc actgtcaact gagatgttcc caacgattac
      241 gccgacggtg ttgctgggga cgtagtacgc ctcccggaac gccgccgcgt cctgggcggt
      301 aaccttctcg acctcgctca tccaccctac aatcgggata cgatatgggt gggcctgcat
      361 cgcggttgcc atgaactgct cgtaaagctt gccttccggg tcgtcgtccg ttcgcatgcg
      421 gcgctcctct gctacgacgt cccgctcgct gtaaaactcc cgcatcacgg ggttcatgaa
      481 acgttctgat tccagcaaag cccacaactc gagtctgttg gcgggaaagg actcgtagta
      541 gatcgtgtag tcttttcccg ttcccgcgtt gttgtctacc ccaccgttcc tgtcgattat
      601 tcgcgaaaac tcgtccttca cgatgaaggt tctatggatc gccgtgtagc gctggaggtc
      661 cgccaaatag ccttttgccc gggcgtacgc acgaagagac gggtaccggt caggtgccgt
      721 gcgcagggtt tccgcgaggg cattggcgag cgctcgggtg atttcgtaat cctccgaacc
      781 ctggttggca acaaccactt cgcgggcagt tctccgttcc agcgcttcaa gagccccgcg
      841 gtgcgcaagc gggacagttg cgagtgtctg ggtgtacagc acggcgacac tgtcgatatg
      901 gcgcatgatc ccctgttcgg cggcgaaatc caccgtgccg atgcgcggcg tgcctttgaa
      961 cgccatgtgt tcgaaaaggt gcgcgaggcc ggattggccc tgaggatcgt ctactcctcc
     1021 aacgcggaca atcactacgc cggagaacac tggcgcggtg cctctcttga cgaaaagaaa
     1081 cttcatcccg ttgtctagcg tgtgctcaac gacccgttcc tcgagtggct gcgcataacc
     1141 tggggctgcc ggcaacacac acgtcgcgca gaccaggaga aaaacgattc gtaccaacag
     1201 aggactctcc ttcatggtgt ttctgtaccg gagggattac cgtgtagtga tcgatatggc
     1261 accgaggcct gaagttgacc gcacgccgca gcgatgtcgt cgcccttgct ccaacgtacg
     1321 gtgaccgtca gattcctctc ccagagaata cgatgaaaac ggtcaattcg ttcctggctg
     1381 ggacggcgga atggctggcg gggtacagga ttgaaaggaa tcagattgat cttgcagggg
     1441 aatccggata ccagttcggc cagtcggcga gcgtcctcgt ccgagtcatt tgttccctcg
     1501 ataagcgcgt actcgaaggt cacccgcctt cggggagaca ctcccgccat gtgccgcgcg
     1561 gcgtccagaa gtgttttcaa tggatatttc ctgttgatcg gcatcaacga attgcggact
     1621 tcgtccgtcg tggcgttaag cgagatcgcg agacccactt tgggaagatc actggccaat
     1681 cggtatatca tcggcactac gccggctgtg ctgatcgtca ctctggtggc ggaaatcgcc
     1741 aaccccatct cgaggccgga cagttttgtg gcttgcaaaa cagcctcgta attgttcaaa
     1801 ggttctccca ttcccatgaa aacaacgttc gtaacgggat gctcatattc ctctccggtt
     1861 gctccccagg ctcgaaagaa acgggcggcg tggtgaatct ggtcgagaat ctctcccacg
     1921 gtcagattcc gctggaagcc gatctggccg gtggcgcaga aacggcagcc gagcgcacat
     1981 cccgcctgcg atgaaacgca gaccgtgcga cgaccatcgt cgagcatcat caccgtttcg
     2041 atttttcggc cgtctgaaag accaagtacg agcttggttg tttgtccgtc gcgtgagact
     2101 gctcgatgcc gctcggcaag gcactccacc cgcgcctccg ctttgagctg cgacctgaat
     2161 tccttaccaa ggtctgtcat ctggtcgaaa tcagcgacac cttttccgtg catccagcga
     2221 aggagttgat tggtccggta tttttccttt ccgaaacgtg ccaggaactc gcgcaattcg
     2281 tcgggcagga aacctttcag gtcgggtttt tctgcacaaa cagcggggtg tgccagagct
     2341 gaggcacgat cgaaggattc ttcgggaatg gttgaaagca tattcgaaaa ataccctcgc
     2401 cgatgtcatt ccgccagcgc tccgttcacc ggtcgatgcg ctgcttgacc gttcttcgca
     2461 aatcagggta tacttcaaaa ctaccgaaat gcgcgtttta cgccggaggt aaccgtggtg
     2521 taccgatttc tgttggctgt cgttgcgacg tctgtgattt gcgtgtttgt tcaggcctgc
     2581 acgccgtcgc gctctttgcc gcagatttcg actcgagaag ttcccgatcc gttcgtcagg
     2641 agcactcaag atgcctcgga cgacagcctc gttcgagcaa aatggatgtg ccgtacaacg
     2701 gttcccgacc ctttggcagg gcaggaagac gggcccaaga tccggtttga agcaacgctg
     2761 tacggtgaag atgtcatagt agcggaagtt gtcaaagagt gccgcgggcc ggcaggtctg
     2821 gtcgcctctg acacgtgtgt gtcggcttct cgtgaacgtt accggagcgt tcaccgcgac
     2881 gacacccggc tcagaatccg tctggacctg gaatcaagtt tcagcgtgaa ttccctgaat
     2941 tcacggtttt gggatgtgta tctgaaagac gagcgaggca ttgcgtacga acccgtcgaa
     3001 attgttcaga atgccccggt cattgtccgc gaggacaccc tcccggaacc cggtcgtgcg
     3061 ccggtgagag gcgcggtgta ccgccgcgcg atcgacatgt actttcctcg ggaagacgcg
     3121 tttggcacag atgtcatttc cagttcaacg cgtgaagtac gacttgtcgt gtctcagaac
     3181 cggcgtgaat tgaccacgtt tgtctggcgc ataaaaagaa ctggcgactc cggaaccaca
     3241 aggactggca gtgcacaacg tgcgccatcg ggtttctgat cgcaccggat tggttcagac
     3301 ccatgaacac ccacgaagaa ctggtcgaaa aggttgagac acttgccgaa agggatggac
     3361 gtttccgtaa ggaagcctac ttctttgtgt ttgaagcgct ccagttcaca gtcaaatccc
     3421 tcaacctgac aggcaaacag cgacacgtga cagggcagca actcgcccat gggattaagg
     3481 aatacgctct tgaccggttt ggcccgatga cgttgagcgt gttcgagcac tggggcgttc
     3541 ggtctacgtt ggatttcggc gaaattgtgt tcggcctggt tgaggccggt ctcatgggga
     3601 aaaccgagga ggattccatc gaagatttcg tggacgttta cgacttccag aaagccttcg
     3661 actggcacaa cgcaattggc agaaagctgc gcaaatgaca tccgaacaac cgcagcacac
     3721 aggcaagcct ttcgtccttg cgagttgcag tattcttccc gagaaatgga acaaagaggg
     3781 aaacgccgag aagatgctct ccttcatgga gcgatcgctg cggtgcggtg ctgacgtcat
     3841 cgcggtgccc gaaggctgcc ttgaaggcta cgtggtaatg gaagcaatcg aagggaaccg
     3901 gggaccggaa ctgttgaaca tcgccgaacc ggaagatggt gaaatgatcg ggcgtttccg
     3961 gagtttctgc cagaagcacc atgtgcacgc gctgatatgt ttctgtgaga gggaaaggaa
     4021 cgaggctttc aacaccgccc tgtggattgg ccggaacggc gaaacagtgg gcaaataccg
     4081 taagacacat ctcaatgaag gatataaccc ggatcaccct ttcaacagta ccgggaacgt
     4141 aattcgtgcg ttcgacaccg agttcgggcg cgcaggagta atgatttgtt ttgaccggcg
     4201 agttcccgag gttgccaggt gcctcgccct cgatggtgcg caattcattc tgaacccttc
     4261 ttacggcgtg tacaagggat ggaacgactc cgttctggtt gcgcgtgcgc acgaaaatca
     4321 ggttccagtg gtatttgttc atccgaacaa aacgttggcg gttaacgaaa aaggcgatat
     4381 cacgttctgc cgcgaagaaa cggatgtgat ttctcatctg acggttacgc cacggacctc
     4441 gtcctcgctt ctcctttcac tccgtcgccc cgaactgtac gggcggctca cctccctcag
     4501 atagttcaca ggggccaggt gccgaagttc ccaacggtgg cccgcagcaa acgaaatcgt
     4561 tataatctct catgccgtct gcgccgcagg tgtggcgctt tctgacgggg gtgcgcaacg
     4621 agcaaaaagc agtcgggagg taacattcaa tggcgggcaa aagcatctgc cacaggatca
     4681 tcgagcagca ccttgtcgcg ggcaaagtaa ttcctggaac tgaaattgga atccgcatag
     4741 atcagacgct cacccaggac gcaaccggaa cgatggccta cctccagttc gaagcgatgg
     4801 gaatacccag gacaagagcc ggattgtcgg taagctacgt tgatcacaac atgctgcagg
     4861 atggctttga aaacgctgac gaccatcggt acttgcaatc cgtggcccgc aaattcggcg
     4921 tgtatttcag tcggccgggg aacggaatct gtcatcaggt gcacagagaa cggtttggtg
     4981 ttcccggaca gacgcttttg ggaagtgaca gccacacacc gaccggcggt ggcatcggca
     5041 tgttggcgat gggtgccggt ggattggacg tcgcggttgc catggcaggg catccgtatt
     5101 tcttcccgtg tccccgcgtg acgctcgttc ggctctcagg ccggttgcgt ccgtgggtag
     5161 gaggaaagga tatcatcctc gaactccttc gccgcctttc ggtgaaaggt ggcgtgggcc
     5221 gcgtaatgga atacggcggt ccgggtgtgg ccacgcttcc ggtggcggat cgttttaccg
     5281 tcgcgaacat gggcgcggaa ctcggtgcga ccacgtctgt gtttcccagc gatgagctga
     5341 cgcggaagta tcttgaagct caaggccggc cagaggcgtg gattcccatg tatgcagacc
     5401 cggacgcaac gtacgaggct gtggtggaaa tagatctgga taagttggaa cccatgatcg
     5461 cccggccaca tagccctgat aatgttgcac cagtgcgtga tcttgagggt acgcccgttc
     5521 agcaggtctg cctcggcagt tgcaccaact cctcctacca tgatctccga atagctgccg
     5581 cgatgctccg tgggaggaca ataagcccag ccgtctcatt gacggttacc cccggttcaa
     5641 ggcaggtcct ggaaatgata gctcgtgacg gctcgctcgc ggatctgatt gctgccggag
     5701 cccgcgttct ggaggccgcc tgcgggccgt gcatcggaaa cgggcaggct cctccctctg
     5761 gagcgaacag cgtgcggagc ttcaacagga atttcgaagg gcgttcggga acgcgcgacg
     5821 cgggggtgtt cctggcaagc ccggaaacgt gcgttgcgtg cgccatcacg ggcgtcatta
     5881 cggatccgcg ggatctcggg atcgaatttc ctgagattcc cgaaccagaa agctattccc
     5941 ggaatgacag tatggtcata ccgccttcga ccgaaccgga agaagtcact gtgtcacgag
     6001 gtccgaacat cgtatcgctc ggttcgttcg aagcgatggc agataccctt cgagggcgcg
     6061 ttctgatcaa agtcggagac aacataacga cagaccacat aatgccggcg ggaagatggc
     6121 tgaagtaccg ttccaacgtc cccaaatatt cggaagcgac cttcaacctg atagatccgg
     6181 attttccaaa acgttccagg gaatggggcg gaggactcat cgttgcgggt gagaactacg
     6241 gtcaggggtc gagccgcgaa cacgcggcgc tggtgcctcg ctggatgggt gtgaaagcgg
     6301 tgatcgcgaa gtcgtttgcg cggatccacg aggccaatct ggtcaatttc ggtattctcc
     6361 cactggtatt cgcggatccg gatgagtacg gacggattca gcagggcgac acgatagaga
     6421 ttccgcacgc gcgccaggcg gtcgaaaacg ggttgaatgt catcgaagcg acgaatctca
     6481 cgcagggtgc caaattcggt ctgagggtta atctcacgga gcgaggtcgg aaagtggttc
     6541 ttgccggcgg catgctgaac ttcgttcgga gttaggagct atccttggag aactcaccga
     6601 gtgagaatca gttgctcgtc ggtgcccaca tgtccaccga gggagggtta ttccgcgcgg
     6661 ttgagcgtgg cgcacagata gggtgcaccg caattcagtt gttcacaaga aatccgagcc
     6721 agtggttttc tcctcccctt tcggaaaaag acgtgacaga gttcagggat gcggtctcac
     6781 ggacgaaaat ggctgcggta gtggcgcacg attcttatct catcaacctt gcgagtcccg
     6841 atgcatcatt gagaaagcgg tcgattgcag cgatgcttga cgaactcgat cgtgcgtcag
     6901 ccctgggtat cccgcttctt gtgacccatc ttggggcgca tactggtcag ggagaggaaa
     6961 acggactgca gacgcttgcg gacagcctca acgaggtgat tgacgcgcgg ccggatgctc
     7021 ctgtttctgt gtgccttgaa accactgctg gtcaggggac ttccctcgga tgggcgtttg
     7081 aacaaatcgc gtgggttttg tcaaaattgt cggcaccggc gcggtttcga acctgttttg
     7141 acacctgcca cgtgtttgct gcggggtacg accttgcgac gcgcgacacg tacgaaaaaa
     7201 cgtggcgaga gtttgataag gttgtgggaa ttaggtatct tgcagtaatg catttgaatg
     7261 acgcaaaaaa agagcttggc agtcgtgtag atcggcatgc gcacatcgga aagggcgaaa
     7321 ttggcgaagg ggcgtttcgc cggataatgc aggacccgga gttgaggggc gttccaaaaa
     7381 tcctggaaac ccccgaatca gaaacgatgc acgctgtgaa tctgagtctt ttgcgagaac
     7441 tcgcggcacg cacgggctga ccttcgcgat atcaaaggat gtcggccttc tagggaggaa
     7501 agcgacgatg gatggggctt caaaaggaat gacgcacctt ggccgaggca tcgttctcgc
     7561 tctttttgcc gtgtgtatcg gcttcggtgg ttgcggcaga cgcggcagcg gcgaatccga
     7621 taataccgac ctgtcaaagc aacttgtcgg aacctggttt gcctacagag ataacgtcat
     7681 tgagaatatg tggaccttcc gggaagacgg tacatgtgtg aatgacgggt ggcccggcga
     7741 cgtccaggcc ccaccgtacc accttgaagg aacgtattcg gtcgagacgg cgaagattac
     7801 cgtcattttc aaactcgagg aagggctgac cgataccgtg atcctttccg accccgacat
     7861 aacaaacgac cgtcttgtat actccgcggg cggaattcct gttgcctttc tgcgcgagcg
     7921 aaccgccgcg gctgcggaat cggcgccaac ggcggatgaa ggggcccctg ttcccgccct
     7981 ggcagactcg attgtcgggg gatgggtcgc ctttgtcgga gggataccgg cgaacacgtg
     8041 gcaattcaac caggatggga cgtttgtaaa cgaaggatgg acacagctct cgccgggctc
     8101 ggttatcctg aaacgcgcgt accaggtttc cggtaagttc gccgtaactg gttcgcgggt
     8161 tctcctgacc aacgaacgca tcgtccattt tggcaccggg ccgcagggtg agaactctga
     8221 agttgctgtt tcgcagaaca tcgtgctcta tgacgtgacg gtatcacgcg gacgactggt
     8281 gtacacgaac gagggggggc tccccgtcgc attccgtcgc ggatacgtaa cgccaaccga
     8341 gtggtaagcg cgggttctcc tctccggcat cgctgaggag gtccccgtca gcaatgccgc
     8401 gtgctgttcc ccgccacccg ttcttccggc ccgccagggg gctcttttga aaatgcctga
     8461 ccccggcaaa actctacaga atgtcctgct cggggttttc ccggagctct cccctgatag
     8521 ctgcacagaa ctgttcgtgc ggcatttccc gcaacatcaa gaacgcgtct cccgtgcaat
     8581 tgagactctt gtggggttgc ttccttcgca gacggtgcgc ctgctggtgg aaacttgtgt
     8641 ggtttccccc gatcccggcg ccgcgatctc ctgtttgtct gaacttgttc aagcggcacc
     8701 ttcctcgttc cgtgacgtgg cccctgaaca ggcgagaagg ctggcctcgt ccctctcagg
     8761 tggggccttt ctcagggaac ggatcctcag acacgcaacg cgagtcttct ggttggttga
     8821 cgaatcggga cggacgttct cccggccgga actcgaacga gaaattgcgg agacccgcga
     8881 gggaagcgct gggtgggacg cactgcgcgt ctgccacgat ctccacctga tgcgtattgc
     8941 cgttgcagat tgtgccggca cgctgggtat cgtggagata acagaacaac tttctctcct
     9001 tgccgaggtg acgatcaatg aggttgccca gcggcaggcg caggcgctca gagcgcggta
     9061 cggagatccg ctccaacagg acggcgcaaa cgcggaatgg tgtgtgatcg gcttgggcaa
     9121 gttgggggga aacgaactca acttccgttc agacgtggac gtgatgttcg tatactccgc
     9181 ggaaggatac tcgacgggag gaacttccgg ggcggtaccg gtggctcagt ggtacacccg
     9241 gtgggcagaa ggaatattga gtgtccttac ggaagtaagc tcgacggggc tgctgtaccg
     9301 cgtcgatacg cgtctccgtc cagatgggca gtctggcgca ttggtgcgca gcattgcctc
     9361 gtacgtccat tattacgagc agaggggcga ggtctgggag aggcagatgc tcgtcaaagc
     9421 gcgtcccgtc gcaggctcag ttcgcatggg gcaaaagctg ctcgattttc tggagtcctt
     9481 tatttacccg cgaagcctcg taacaagtcc acgggaggaa atccacaggg tcaagaatcg
     9541 gattttgcaa catcttgacg cgagagcaac cagttcaaat ccggcgaatg cagaacggaa
     9601 cctcaagctg ggccgggggg ggctgcgaga catcgagttc gtggttcagt gtcttcaact
     9661 ggtcgtaggg ggcagcgaca aagccgtacg ggagcagaac acgctcgcgg cgatcaggag
     9721 actccaccag cgtggtgtgc tcagtgacac tgagcaacga gcgctggatg aggcataccg
     9781 gctcttccga cgcacggaac atcgtctgca gatggcgaca gggcagccat cattcgtcct
     9841 tccttcagag catgacgagc tatcacttct ggcgcgaaga atgggttact ctgatccagc
     9901 cgcgttgatg gcagacctgg aaacggccag gaagaacgtc atgtcggtat atgacagcgt
     9961 tctgggcccg ccggagacag gcacagaaat cggcaagatt ctgaatctcc ccgcaggcga
    10021 tgcccaggct gtctcggaat tggccgggtg cggttttcgc aaccccgagg cggcgcaccg
    10081 aaatctgctc caccttgcgt tcggtcatgg cgatacggtt cctgcttcca cgccttccca
    10141 gcccgtgctc cggctggtgc ctcgtttgct ggatgaactc cgtgggagtg caaacccgga
    10201 tcgggggctc aacaacgtcg agaggatgct cagagcgttt ggcgcagtag acagcttcgc
    10261 tgaccttctg tcgtcgcaca acgcattcct caacctgctc gtaacaatcg cagcacgcag
    10321 cgaagctctc accgaaacga tctccaggga tcctggtctc gtggattgga tgctgtattc
    10381 cggcgccctc ttcgccgaaa ggcacccgga agacgtcgac ctggaatccc gggcggccgt
    10441 tgccggtctg cccagtgctg cattgcagat tgccgcactt cattcgtttc gaaagcgcga
    10501 gaacctgcgt gtgggagtcc gtttcctcat ggacctcgcg aacgaagagg agacatctga
    10561 gcagctcact gccatcgcgg aatcggtggt gaagcgggtt gtctctgtcg cgagcagatc
    10621 gttggcgggg gcagctgcag aaaaccgttc ctaccagtgg gcaatcatcg gcttgggaaa
    10681 actgggcgga cgcgaaatga acttcgggag cgatctcgac attttcttcg tacacgcaga
    10741 agagcaagac gaaggccaat gtgtggacta tgttacttcg ctcgcgcagg aggttctccg
    10801 gctcctgtgc gaaaacacgg agtacggggt tctttatgaa gtcgacacca ggcttcgtcc
    10861 cgaaggaaag cacggtccat taagcctttc gatcgacggt ttccgcgagt accttcgcaa
    10921 acgtgtggca cagtgggagc gtcaggcact gaccttctca cgtgtgatcg tttccgcgcc
    10981 gcagccgcgc ttcggagaag ttgtcgagga agcaatctgg aacgcgattg cggtgaaacc
    11041 cacgagggag atatttgaga gcgcgctttc gatgcggttg cgcatgcagg aacaggcaca
    11101 gagcaactat ggaacgcggc caaacataaa gaccggcttc ggcgggctgg tggacgcaga
    11161 gttcattgcg caattgggga ggatgatcgg cggagtacgc ctttcggaag ggcgaaaagg
    11221 cgacaccaga gccactctcg aatgcatgag atctgcaggc cttcttccag accgggttgc
    11281 ccagacgctg atggatgcgt accacgcgtt acggcggatc cagatgcatc ttcgcgtgaa
    11341 cgactatcgc gcgcacaatg ttctgcccga cgatgaagac gctattctga cccttgcaag
    11401 agggttgggt ttccgggaca ggagtgatct ttccgtgcac gttgaaaaag tgacggaaga
    11461 catgcgcggt gcgttccttt tagccctcga atccctcgcg accgcgaata caaaagcact
    11521 cggaggatcg cttgtctgac cgattcatgg gcggatggcg cgtactcgca atcctgattg
    11581 ccgcagtgat ctcactcgca acgggtccag ccctctcggc ggcggaagag acgcgtaatc
    11641 tccgaatcgg tcccattgcg gaggtccggg tctcgccgca attctcggtg tctctggtga
    11701 tccagcggag agggcgctcc ttacaggaac tggggaagca tttccttgct cgcccgcagc
    11761 acgcgtctcg ttacacgcat cgggaccaga tgacagtccc gttccctgca ttgagtgatc
    11821 cttacaaggc aatcgtcgct ctgcgtttgt ttcctgagga cagcctttcg caagacggcc
    11881 tcg
//
LOCUS       DAOTNG010000061        11845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5144_length_11845_cov_5.411790, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000061 DAOTNG010000000
VERSION     DAOTNG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_11845_cov_5.411790"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5179_04475"
     CDS             complement(<1..504)
                     /locus_tag="P5179_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRS94508.1"
                     /translation="MEQGFLNIAHRGFSGQYPENTIIAFRAALELGCTWLECDVRRTR
                     DGCFVVIHDQTVDRTTNGTGRVDSLTWNEIMRLDAGSWKDPKFSGERVPLLDELLTLV
                     SGQAQLVIELKVGKDSVPGVVSVVSRHCAFGWTVASAFEWEVLETVRREAPRWRTTWL
                     TTFEDLGP"
     gene            complement(518..790)
                     /locus_tag="P5179_04480"
     CDS             complement(518..790)
                     /locus_tag="P5179_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRS94509.1"
                     /translation="MLPNVGPWEMIVILLVAFLVFGSKRLPEVARGIGKGIREFRREI
                     SGVTDEINGTRDAVRNAPPPPPVSASKEESEKKDEDVRNPSDPVIL"
     gene            complement(817..1590)
                     /locus_tag="P5179_04485"
     CDS             complement(817..1590)
                     /locus_tag="P5179_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRS94510.1"
                     /translation="MKIGLKRVRGLLAKENRKSLGLFLVEGVRLVEEVLASPLTVECL
                     FHVEADPDSRLFRVIEAAASDGIPTQSITQKELDAICDTRTPQGVVAVARTPTWTEED
                     VWKSGTGDVVVVDNVRDPGNLGTLLRSAEAAGARGVLLMPGTVELTSPKVVRGSMGAF
                     FRLPVSYTHSIDDVLRGARSAGIPLVATSSHGGEPASLLLKWDAVALVLGGEAEGVAR
                     DWTVVCDCIVSLAQKRGTESLNVAVAGSIFLFRHLWRAV"
     gene            complement(1594..2634)
                     /locus_tag="P5179_04490"
     CDS             complement(1594..2634)
                     /locus_tag="P5179_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94511.1"
                     /translation="MKRMRSNAVGVRCQLIAVCVLFSALPESVRAVQVPANFVLAQEA
                     ALRSEFARAESLAVVMKREQPADPTADYAMAAIEQMRLFGCEGTMSPAELKRRVQIAV
                     RAGREQVARRPEAIWPRYVFGLSLGLSAVIAADEGSYWHAYRAGTESVEQLEEVLRRE
                     PGFSDALLALGAYHYWRGAAMRNWTWLPFIADTRSQGIREMKRAWREGATTQNTAPGM
                     IVWALLKDADYHEALAISSEIAARYPANRAFVTARADALFGLRRWEEAAAAYTQAVGL
                     YSGNQIRCPGSVEARAKRGIALAEMGKCSDALPDLRAAVLSPAHVGYGRDFKATQNRA
                     RVLLERCLEKGP"
     gene            2794..3414
                     /locus_tag="P5179_04495"
     CDS             2794..3414
                     /locus_tag="P5179_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94512.1"
                     /translation="MRKPLTKRQLAIYEYIAAQIRQRGIPPTIREICDQFGMRSSNGA
                     REALAALARKGYINRRERLSRGIELANPFDSLECSGTPIRVPLLRNPVNGARCVDEAT
                     GEKVLFIDSSFLPGTGRAFALLVPDGSLAKYGIRKGDLAIATECHEGQPGEILITAID
                     SRLVLVRVPATSDPEPSQQVPESHGVSPVFGKVNCVVRRFTGVETG"
     gene            3678..4628
                     /locus_tag="P5179_04500"
     CDS             3678..4628
                     /locus_tag="P5179_04500"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRS94513.1"
                     /translation="MKINKVVIPVAGLGTRLLPATKSQPKEMLPVGRKPVVQYVVEEM
                     VEQGLRYLLLITGSNKHSIENHFDNDPMLVERLLSTEDDDLLREVDLRPEGAQIFYTR
                     QMIPPGAKKPAGLGDAVRHARAFVNDDPFVVALGDTIIHTDGKPDLISRMTQTHVSND
                     AVATIAVWLVEHDATRRYGVVRPEEGADLSQPFRITDIVEKPTPDQAPSRYAVAARYV
                     FGGQIFDALDATLPGHGGEIQLTDAIRRLVREGKPVYCVPLRPNETRYDIGNPESYFK
                     AFVDFAFDDPQCGEKLRRHARALLERYERAEGLSRGGDKS"
     gene            4625..5506
                     /locus_tag="P5179_04505"
     CDS             4625..5506
                     /locus_tag="P5179_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRS94514.1"
                     /translation="MTLREHIKEGILLLDGAMGTQLTARGAKGNNERWGVEHPEFLAD
                     VHRAYVDAGAQAVITDTFGGNSAKLAKAGLGDRADELNRRLVEIAREAVRGRAWVLAD
                     VGPTGQFVEPYGDLTESQMTDVFRRQIAVLLEAGADGIIIETMMDVTEAVCAVNAARS
                     LAAGIPVLVTMTFDVNPRGFRTLMGVSPAQAASRLAETGPDAVGANCGGLLPVQYAAL
                     CSEMSAATSLPIIAEPNAGLPELEDGVTVFKEPPETFGAIVPDLIASGVKVMGGCCGT
                     GPAHIQAMSNALKKMLK"
     gene            5503..6804
                     /gene="larA"
                     /locus_tag="P5179_04510"
     CDS             5503..6804
                     /gene="larA"
                     /locus_tag="P5179_04510"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRS94515.1"
                     /translation="MIVELKYGRRGLRVEIPAQVSCRVLRANTAGALADEEASVNASL
                     HHPIGSRPLAEIARSRISHRHGVATASITVSDITRPVPNSLIVPQVLNVLNTAGIADE
                     HIAVIVGTGLHRASTEQEIAEILGADVPRRCRVTSHVARNRSTLAYLGKTSRGTPIWV
                     NREFVEADVRIAVSLIEPHFMAGFSGGRKAVCPGLAGVDTMRVLHGPEILSHPNVREG
                     CLAGNPFHEEATEIARRAGLDFTISVTLNEQRRITGVYSGDPETAHAAGCAAAARNTT
                     AQIPEPVDAVITTSAGYPLDLTFYQSVKAMTAALPILKKGGTLIVASSCDEGIGSPDF
                     TELMLGTPSLKEFRDRLKDPGFFVPDQWQFQMMCRALETAQVLYFSTGIPADILAKLF
                     VTPIASVEEGIARALERHGSGASMAIIPEGPYVMPAVFPSR"
     gene            6996..8357
                     /gene="gdhA"
                     /locus_tag="P5179_04515"
     CDS             6996..8357
                     /gene="gdhA"
                     /locus_tag="P5179_04515"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRS94516.1"
                     /translation="MASFVDEIMAQVIAKNPTEKEFHQAVTEVAESVALVYDKFPEFR
                     KAKVFERMVEPERVIMFRVPWVDDKGEIQINRGFRIQMNSAIGPYKGGLRFHPSVTLG
                     VLKFLAFEQVFKNSLTTLPMGGAKGGSDFDPKGKSDGEVMRFCQNFMNELFRHIGHDT
                     DVPAGDIGVGGREIGFLFGQYKKLANQFTGVLTGKGPAWGGALIRPEATGYGATYFAA
                     EMLATRGEKLEGKTVLVSGSGNVAQYTVEKVNDLGGKVVTMSDSSGYVYDEAGIDAKK
                     LKFIMELKNERRGRIKEYADAFPGVVYTPVDPNLGYNPLWNHKADVAFPSATQNEINE
                     RDAENMVRNGVICVAEGANMPTTLEGVRVFLDAGILYAPGKAANAGGVAVSGLEMSQN
                     SMRLPWTREEGDNRLRGIMKSIHKTCATTAEQFGTPGNYVNGANIGGFMKVGRAMLDQ
                     GIV"
     gene            complement(8406..9242)
                     /locus_tag="P5179_04520"
     CDS             complement(8406..9242)
                     /locus_tag="P5179_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRS94517.1"
                     /translation="MSRKVVLPTWYYRQYPSDFRRGEEATLGILGWARRDCTVPLDEM
                     ALVLMHFWNVGFPGGPEWSENSPHVLVRQAQEYVGRCVAQFTRSMPPILKAARESGMT
                     IVHVASGEDYAKRYPHYQRTCAEVNLEAPQKQPGAIRPQTTDIPTNVERYGPGWADFK
                     DAPYDFPSIVRPEAENELLCVHTHQLNSVLRNRGIWHLTYCGFAINWCLWHSPCGMVD
                     MSRLGYTCGCIRQGVVAVENRESVRTKANHDYAMWKTAHMFGFVIDDDDFIRGVQNTR
                     EG"
     gene            9380..10315
                     /locus_tag="P5179_04525"
     CDS             9380..10315
                     /locus_tag="P5179_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94518.1"
                     /translation="MTPKERAVAALECRRPDDIVPTFELVYFLGPEKFGRDWTENIRS
                     MTPKEQDRYLHEHAELHVMIAEEYDYSILRSDYIEAIRIFKKWGLDKTYLLCGEADGT
                     MAIPNGHDMVEVAAALRERPDEMHAQMRRGAEGAKEAGRRFREAGAEAVTMCADYCFN
                     DGPFLSPGMFREFVTPYLTEIIASHRENGLFVIKHTDGNIMPILDQLVEANPHALHSI
                     DPQARVDLAEVKRITAGKICLCGNVNCGLLQTGTDQEVREDVLRSLRDGMPGGGYIFS
                     TSNCAFRGMPLERYELMMSLRKEYGRYDKPAFPMA"
     gene            complement(10356..>11845)
                     /locus_tag="P5179_04530"
     CDS             complement(10356..>11845)
                     /locus_tag="P5179_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94519.1"
                     /translation="QVPSGEFVFTPIRFCSVYGTHEMAWRLECLLTAFLCVREALTSE
                     ERSRYRDTLLRAMEFLRETPCEHRCNRGVTWCAVMAMCWRATGDERYLADAQAVWERV
                     APDVFLENGQIFEGCGPCNGYSMISYEYLLRYRTATADDSLDPVIERSTDWVTRMYTD
                     RYTTFRGVSTRQDIPDATHKAVPLLLGLERLSSRAPWYSVMADSLLDRIELGYETAPA
                     QHGGIAWVAAAAAHNSATSNGSNLAARRAPSVRSYSSQATSYATVEQPGYQALIAFRS
                     LSPRKGLQTWAVRGEAPFIFPEDNQPSCAISWGHDSARDNVTEWDTIREDAGRFPSFT
                     TRTGDGVTSYVVTPMTLVVVYSFDTPRETLIRWCASSRHTGGFHLQNGFVSAIGAKGS
                     LHWWGGEPTISEDGFVVEFRGDLLTQAFAFSAGGFRRIGSIASEDGLITLSWEDDAGS
                     YAAVANHAPRPLPLPPDVNRPQNEHATLLDPCEVRVFCNDRSPVQ"
BASE COUNT         2584 a         3483 c         3451 g         2327 t
ORIGIN      
        1 gggaccgaga tcctcgaagg ttgtcagcca cgtcgttcgc cagcgcggag cttcgcgccg
       61 caccgtctcg aggacctccc attcaaaggc actggcaacc gtccagccaa acgcacaatg
      121 ccttgaaacc acggagacca cgccgggtac cgagtctttc ccaaccttca gttcaatcac
      181 caactgcgcc tggccggaaa ccagcgtaag aagttcatcg agaaggggga cgcgctcgcc
      241 ggagaacttc ggatccttcc aggaacctgc gtcaaggcgc atgatctcgt tccatgtcag
      301 ggagtcaacc cggccagttc cgttcgtcgt ccgatcgacg gtctggtcgt gaatgaccac
      361 gaaacaaccg tctcgcgttc tgcggacatc gcattcaagc cacgtgcatc ccagctccaa
      421 cgctgcccgg aacgcgatga tcgtgttttc cgggtactgg ccgctgaaac cacggtgggc
      481 aatgttcaga aatccctgtt ccatctcggc atccgggtca gagaatcacc gggtcggacg
      541 ggttccgaac gtcttcgtcc ttcttttccg attcctcctt cgaggcagac acaggcgggg
      601 gcgggggagc attcctcact gcgtcgcgtg ttccgttgat ttcgtccgtc acgccggaga
      661 tttcgcggcg gaattcgcga atccctttcc cgattccccg cgccacctcg ggcagtcgtt
      721 ttgacccgaa gacgagaaac gcgacgagca aaatgacgat catctcccag ggaccgacgt
      781 tcggcaacat gcagaatcct ttcctatccg accggatcag actgcgcgcc acaggtggcg
      841 gaagaggaaa atcgacccgg ccacagcaac attgagtgac tccgttcctc ttttttgtgc
      901 cagagatact atgcaatcac ataccacggt ccaatcgcgc gcaacgcctt ccgcttcccc
      961 gccgagaacc aatgccaccg catcccattt caatagcaga ctggcaggtt ctccgccgtg
     1021 tgacgacgtg gctacaagag gaatccctgc gctccgtgcc ccacgaagaa cgtcgtcaat
     1081 tgagtgcgtg tacgaaacgg gaagacggaa aaacgctccc attgagccgc gtaccacttt
     1141 gggactcgtc agctcaacgg tgcccggcat gaggagtacg ccgcgcgccc ccgctgcttc
     1201 cgccgaccgc aggagggtgc caaggttgcc ggggtcgcgc acgttgtcca ccacaacaac
     1261 gtccccggtg cccgatttcc agacgtcttc ttcggtccac gtcggtgtac gagcgacggc
     1321 aacaacgccc tgcggcgttc gcgtgtcgca gatcgcgtca agttccttct gggtaatgct
     1381 ctgcgtcggg attccatccg acgccgccgc ttcaattacc cgaaagagcc gcgaatcggg
     1441 atctgcctcg acgtggaaca ggcattctac ggtaagcgga gaggcgagga cctcttcaac
     1501 aagccgaacg ccctccacca gaaacagccc gagagatttc cggttctcct tcgccagaag
     1561 cccgcgcacg cgtttcagac caattttcaa ttctcatggc cctttttcga ggcatcgctc
     1621 aaggagcaca cgagcacggt tttgcgtggc cttgaaatcg cgcccatacc ccacatgcgc
     1681 cggcgatagc acggcagcgc gaaggtctgg cagcgcgtcg gaacatttcc ccatctccgc
     1741 cagtgcgata cctcttttcg ctcgcgcctc cacagaaccc gggcaccgga tttggtttcc
     1801 agaataaagt ccaacggcct gtgtgtatgc cgccgctgct tcctcccacc gccgtagacc
     1861 gaacaacgca tcggcccggg cagtaacaaa tgctcgattc gccgggtagc gtgccgcaat
     1921 ctccgaggat atggccaacg cttcgtggta atccgcgtcc ttcagcaacg cccagacaat
     1981 cattccgggc gcggtgttct gcgttgtcgc cccctccctc cacgcccgtt tcatttcgcg
     2041 tatgccctga ctccgcgtat ccgcgataaa gggcaaccat gtccagttcc tcatcgctgc
     2101 tccccgccag taatgatacg ctccgagagc gagcagcgcg tcagagaaac cgggttcacg
     2161 acgcaatacc tcttccagtt gttccacact ctcggtcccc gcgcggtacg catgccagta
     2221 cgagccctcg tccgccgcaa tgacggcgct caggccgagg ctgagtccga acacgtaccg
     2281 gggccagatc gcttccgggc gccgcgcaac ctgctcacgg cctgcgcgga ctgcaatctg
     2341 aacacggcgt ttgagctcgg ccgggctcat tgttccctcg caaccaaaaa gacgcatctg
     2401 ctcaatcgca gccatggcat aatcagccgt ggggtcagcc ggctgctcgc gcttcatgac
     2461 aacagccagg ctctccgcgc gcgcgaactc cgaccgcaga gctgcttcct gagcgagtac
     2521 gaaattggca ggcacctgca ccgcgcgcac agattccggc agcgcggaga aaagcacgca
     2581 caccgcgata agctggcagc gcactccgac cgcattgcta cgcatccgct tcattaaccc
     2641 cagccatacg cctgagggtt gcggtttctt tccccgcacg ggcaccctat ctttgaaatt
     2701 ggtcggcgct tcggggaggc ttccagaacg tgttcccaag ggacatatca ccaccgccaa
     2761 cgacgtgatt tcaaatctgt acggtcagcc ggaatgagaa agcctcttac caagcggcaa
     2821 ctggcaattt atgaatatat tgccgcacag attcggcagc gcggtattcc ccccactatc
     2881 agggaaatct gcgaccaatt cggcatgcga tcttccaacg gggcacgaga ggcgctggca
     2941 gcacttgcga gaaaagggta catcaatcga cgagagcggc tttcccgtgg catcgaactg
     3001 gccaacccgt tcgactccct cgagtgttcg ggaaccccaa tccgtgtgcc tcttttgagg
     3061 aatcccgtta acggtgcccg gtgcgttgac gaagcaacgg gtgagaaagt cctgttcatc
     3121 gattcgagtt ttctgccggg cactggcaga gcgtttgcct tgctcgtacc cgacggttcg
     3181 cttgccaagt acggcatccg aaaaggagac ctggcaatcg ccacggaatg ccacgaaggg
     3241 caaccgggcg aaatcctgat cactgcaatc gactcgcgcc tcgtactcgt ccgcgttcct
     3301 gcgacctccg acccggagcc ttctcagcag gttccggaga gccacggggt ttcgcccgtg
     3361 ttcggaaagg tgaactgcgt agtgcgccgt ttcaccggcg tggagacggg gtaggcccaa
     3421 ctgcgaatcg gcagatttgc gagagctttg tgcagcgcgg acaggtgtgg tctccgcaaa
     3481 acggagccaa ggggtgggaa acatgcactg gcagttacgc gcgcgttggg tatggtcccg
     3541 accgttcccc ggtgagaaac ggtggggcac ccatgcgctg gttccattac tgaaaatcgc
     3601 ttttgtcttc tttcctgggc tcctttttcc caggtgtagg tacactgtag cgaacgcgtg
     3661 acaaaaaggg tgtcgtcatg aaaatcaaca aggtagtgat accggttgcc ggccttggaa
     3721 cgcggttact tcccgctaca aagtcgcaac cgaaggaaat gcttcccgtg ggaagaaagc
     3781 cggttgtgca atacgttgtc gaggagatgg tcgaacaggg gttgcggtac ttgctgctta
     3841 tcaccggcag caacaagcac tcgatagaga accattttga caacgacccg atgctcgttg
     3901 agcgcttgct cagcacggaa gatgatgatc ttcttcgaga agtggatctg cggcccgaag
     3961 gagcccagat attctacacg cgccagatga ttcccccggg tgcaaagaaa ccggccggcc
     4021 tcggcgacgc cgtccgacac gcgcgggcgt ttgtgaatga tgatccgttt gtggttgcgc
     4081 tgggcgatac gatcatccac acggacggca aaccggacct gatttcgcgc atgacccaga
     4141 cgcacgtgag caatgatgcg gtggccacca tcgcggtgtg gttggtcgag catgatgcaa
     4201 ctcgccgata cggtgttgtc aggccggagg aaggcgccga cctgagccag cctttccgga
     4261 taacggacat cgtggaaaaa ccaacaccgg atcaggcgcc aagccgctat gcggttgccg
     4321 cacggtacgt ctttggaggc cagatattcg acgcattgga cgccacattg cctggccacg
     4381 gaggggaaat ccagctcacg gatgcaatcc gacggcttgt gcgcgaaggt aagccggtgt
     4441 attgcgtccc ccttcgtccg aacgaaacgc gctacgacat cgggaaccct gaatcttact
     4501 tcaaagcgtt tgtggatttc gcgttcgacg atccgcaatg cggcgagaaa cttcggcgac
     4561 acgcaagggc tcttctggag aggtatgaac gtgccgaagg cctttcgcgg ggtggggaca
     4621 aatcatgaca cttcgagaac acatcaaaga aggtatcttg ctccttgacg gggcgatggg
     4681 cacacaactc accgcacgcg gagcgaaagg caataacgag cggtggggag ttgaacatcc
     4741 tgagttcctc gccgatgtgc accgcgcgta cgttgatgcc ggagcgcagg cagtcatcac
     4801 ggatacgttc ggcggcaaca gcgcaaaact ggccaaggcg gggctcggcg accgggcaga
     4861 tgagctgaac cgccgccttg tggagatcgc gcgggaggct gtccggggaa gagcctgggt
     4921 actcgcggac gttggtccca cggggcagtt tgtggaaccg tacggtgatt tgaccgagtc
     4981 gcagatgact gacgtgtttc gcagacagat tgccgtgttg ctcgaggccg gtgcggacgg
     5041 gataatcatc gaaacgatga tggacgtcac cgaagctgtc tgcgcagtca atgcggcgag
     5101 gagcctcgcg gcgggaattc ctgtcctcgt gacgatgacc ttcgacgtga acccccgtgg
     5161 gtttcgcacg ctgatgggag tatcgccggc tcaggcagcc agccgactgg cggaaacagg
     5221 ccctgatgcc gtcggagcca attgcggggg ccttctgcct gtgcaatatg cggctctttg
     5281 ttcagaaatg agcgcggcaa cctcgctgcc gatcatcgcg gagccgaatg caggcctccc
     5341 cgaacttgaa gatggagtaa cggtattcaa agagccgccg gagacattcg gcgccatagt
     5401 gcccgacctc atcgcatcag gcgtgaaagt gatgggaggg tgttgcggga ctggtcccgc
     5461 tcatattcag gccatgtcga atgcgctcaa aaagatgctg aagtgattgt cgaactgaag
     5521 tacgggcgcc ggggtcttcg cgttgagatt ccggctcagg tttcctgcag agtgctgcgc
     5581 gcaaacaccg cgggagcgct ggcggacgaa gaagcatccg tcaacgcatc gctgcaccac
     5641 ccgattggca gcagaccact cgcggagatt gcgcggagca gaatttccca ccgacacggc
     5701 gtcgccactg catcaatcac tgtcagcgac atcacacgcc ccgttcccaa ttccctcatc
     5761 gtgccgcagg ttctgaatgt gctgaacacc gcgggcatcg cggacgaaca tattgccgta
     5821 attgtcggga caggcctgca ccgagccagt actgaacagg agattgccga aatcctcggt
     5881 gccgatgttc cgcgccgttg cagagtaacc agccacgtcg cgcgcaacag gtcgacgctg
     5941 gcatacctgg gtaagacgtc gagggggaca ccgatctggg tgaaccggga gtttgttgaa
     6001 gccgatgtcc gcatcgccgt atcgctcata gagccacatt tcatggcggg attcagcggc
     6061 gggagaaagg ctgtttgccc cgggttggcg ggggtcgata caatgcgtgt tctgcatggc
     6121 ccggaaatac tctcgcaccc gaatgtccgc gaagggtgtc tcgccgggaa tccgttccac
     6181 gaggaagcaa ccgaaatcgc gcgccgggca ggactggatt tcactatttc cgtaacgctc
     6241 aacgaacaac gcagaatcac cggagtatac tcgggcgacc ccgaaactgc acacgctgcc
     6301 gggtgcgctg ccgcggccag gaacaccact gcgcagattc ctgaacctgt ggacgccgtc
     6361 atcaccacct cggcgggata cccgctggat ctgacgtttt accaatccgt gaaggccatg
     6421 actgctgctc tgccaatact caagaaaggc gggacgctga tcgtggcttc atcctgcgat
     6481 gaaggaatcg gatctccgga tttcacggaa ctcatgcttg gaactccctc cctgaaagag
     6541 ttccgcgacc gattgaagga ccccggattc ttcgtgccgg atcagtggca gttccagatg
     6601 atgtgccgtg cgctggaaac agcacaggtg ctttatttct cgactggaat acctgctgat
     6661 attctggcga agttgttcgt cacgccaatt gcatccgtgg aggaagggat tgcgcgggct
     6721 ctggagcgac atggttccgg agcaagcatg gcaatcatcc cggaaggtcc ttatgtgatg
     6781 ccggccgtct ttccgtcccg ttgattgatt cccctcgata ttcgttctca ttccgcctga
     6841 ccgattcgcg acactacggt tccgccactc cattttcgtg ccattaatag ccagaagcac
     6901 cgttggcaga cccttgcggc aaaactgggc gaatccggat atttattctt gctttgtcaa
     6961 taacacttca acaacgaacc ccgggagact aaaagatggc gagtttcgta gacgaaatca
     7021 tggctcaggt catagcgaaa aaccccacgg agaaggaatt ccatcaagcc gtaaccgaag
     7081 tcgcagaatc ggtcgcactc gtctacgaca agttcccgga gttccgcaag gccaaagtat
     7141 tcgaacgcat ggtcgagccg gaacgcgtga tcatgttcag ggttccctgg gttgatgaca
     7201 aaggcgagat ccagatcaac cggggcttca ggattcagat gaacagcgcg atcgggccct
     7261 acaagggcgg attgcggttc cacccttcag taacgcttgg cgtgctgaaa ttcctcgcct
     7321 ttgagcaggt gttcaagaac agcctgacca ccttgcccat gggcggcgcg aagggtggtt
     7381 ccgatttcga tccaaaaggg aaaagcgacg gagaagtgat gcggttctgc cagaacttca
     7441 tgaatgagct gttccggcat attggtcacg acacggacgt tccggcgggc gacataggtg
     7501 taggcggcag ggaaatcggc ttccttttcg gtcagtacaa gaagctggcg aaccagttta
     7561 ccggcgttct cacgggtaaa ggccctgcgt ggggaggcgc tctcattcgt ccggaagcaa
     7621 ccgggtacgg tgccacgtat ttcgcagccg aaatgctggc aacgagagga gagaaactgg
     7681 aaggcaagac ggtacttgtt tccggaagcg gcaatgttgc gcagtacacg gtggaaaagg
     7741 taaatgacct cggtggtaaa gtggtcacca tgtcagattc ctcggggtac gtgtacgacg
     7801 aggcggggat tgacgcgaaa aagctcaagt tcataatgga actgaagaat gaacggcgcg
     7861 gaagaatcaa ggagtacgca gacgcgttcc ccggcgtcgt gtacactccc gtggatccga
     7921 atctcgggta caatcccctt tggaatcaca aggcagacgt tgcattcccc agcgcaaccc
     7981 agaatgagat caacgaacga gacgcggaaa acatggtgcg caacggcgtc atttgcgtcg
     8041 cagagggggc caacatgccg accactctgg agggcgttcg tgtcttcctt gatgccggga
     8101 ttctctacgc ccccggcaaa gccgcgaacg cgggcggagt tgcggtttcg ggacttgaga
     8161 tgtctcagaa cagcatgcga ctgccgtgga cccgcgaaga aggcgataat cggctgcgtg
     8221 ggatcatgaa aagcattcac aagacgtgcg cgacgaccgc ggagcaattc ggaactcccg
     8281 ggaactacgt gaacggcgcg aacattggcg ggtttatgaa agtgggaaga gcgatgttgg
     8341 accaggggat cgtctgatcc atcgcagacc gcagacactg atgccaggag aggggtggaa
     8401 gacttctacc cctctctcgt attctgaaca ccacggatga agtcgtcgtc gtcaatcacg
     8461 aagccaaaca tgtgagccgt tttccacatc gcatagtcat gatttgcctt cgtgcgcacc
     8521 gattccctgt tttccaccgc gacaaccccc tgtcggatgc acccgcacgt atacccaagg
     8581 cgtgacatgt cgaccatacc gcacgggctg tgccagaggc accagtttat ggcaaatccg
     8641 cagtaagtaa gatgccatat gccacgattt cgcaggacac tgttgagctg gtgggtgtgc
     8701 acgcaaagca gttcgttttc cgcttccggc cgcacgatag acgggaaatc atacggcgca
     8761 tctttgaaat cagcccaccc cggtccatac cgctcaacat ttgtcggaat atcggtcgtt
     8821 tgagggcgga tggcgccggg ctgcttttga ggggcctcca gattcacttc cgcgcacgtt
     8881 cgttgataat gcggataacg ctttgcatag tcctcccccg aggcaacatg cacgatcgtc
     8941 atacccgact cacgggccgc tttgagtatg gggggcatac tgcgcgtgaa ttgcgcaacg
     9001 caccgcccca cgtactcctg agcttgcctt accagcacgt gcggagagtt ttcgctccat
     9061 tccggtcccc caggaaaacc cacgttccag aagtgcatca gaacaagcgc catttcatca
     9121 agcggcaccg tgcaatcccg ccttgcccat cccagtatgc cgagagtcgc ttcctcgccg
     9181 cgccgaaaat ctgagggata ctgacggtaa taccaggtgg gcaatacaac ctttcgactc
     9241 acgccttcct ccttgtcgtg cgtttcgtcc gacggcgagc atccgtacct tgtgtacaca
     9301 aggtaacgga aaactctgat agtcccgagg acacgggcac caaggtcgtg tcgcaccagc
     9361 aacaaggaga aacaccatca tgacccctaa ggagcgcgcc gtagcggctt tggagtgtcg
     9421 gcgtccggat gacatcgtgc cgacgttcga gctcgtgtat tttctcggac cggagaagtt
     9481 cggacgggac tggaccgaaa acatccggag tatgacgcca aaagagcagg atcgttacct
     9541 gcacgaacat gctgagcttc atgtgatgat cgcggaggag tatgactatt ccatactccg
     9601 ctcggactac atagaagcaa tccggatttt caagaaatgg ggccttgaca aaacctatct
     9661 gctctgcggc gaagcggacg gcacgatggc gattccgaac gggcacgaca tggtggaagt
     9721 cgccgcagca ctgcgggaac ggcctgatga gatgcacgcc cagatgcgac gaggcgcgga
     9781 aggcgcgaaa gaagcggggc gacggttccg cgaggctgga gccgaagccg tgacaatgtg
     9841 tgccgattac tgtttcaacg acggcccgtt tctgagtccc ggaatgttcc gcgagttcgt
     9901 cacgccgtat ctcaccgaaa tcatcgcgtc acacagggaa aacggcctgt tcgtgatcaa
     9961 gcacaccgac ggcaatatca tgccgatact ggaccaactg gttgaagcga atccccatgc
    10021 tctccattcc atagacccgc aggcgcgcgt ggacctggcc gaagtcaagc gcatcactgc
    10081 cgggaagata tgtctctgcg ggaacgtcaa ttgcgggctc ctccaaacag gtacggacca
    10141 ggaagtgcgc gaagacgtct tgcgctcctt gcgtgatggg atgcccgggg gcgggtatat
    10201 tttctcgact tcgaattgcg cattccgagg catgccgctc gaacggtacg agttgatgat
    10261 gtcgctaagg aaggaatacg ggcgttacga caaaccggca ttcccgatgg cctgatcgaa
    10321 tagatccgac gttccgtctt gcctttgacg gtggactact gaactggact gcggtcgttg
    10381 cagaagaccc tcacttcgca ggggtccaac agcgttgcat gttcgttttg cgggcgattt
    10441 acgtcgggcg gcaggggcag tggacgtggc gcatggttgg cgacggcagc gtagctgccc
    10501 gcgtcgtctt cccaggacag tgtgatcaga ccgtcctcag acgcaatgga accaattcgc
    10561 cggaagccgc cggcagagaa cgcgaacgcc tgcgtgagga gatcgcctcg aaactcgaca
    10621 acgaatccat cctcactgat tgtgggttcg ccgccccacc agtgcaacga accttttgct
    10681 ccgatcgcgc tcacgaagcc attctgaagg tggaaccctc cggtgtggcg gctgctggcg
    10741 caccatctga tcaaggtctc gcggggcgtg tcgaaggaat agaccacaac gagagtcatt
    10801 ggcgtgacga cataactcgt gacgccgtct cccgtcctcg tggtgaaact cgggaaccgg
    10861 cccgcatcct cacggatggt gtcccattcg gtcacattgt ctcgggcaga atcgtgcccc
    10921 cacgatatgg cgcacgatgg ttggttgtct tccgggaaga tgaagggggc ctcaccgcga
    10981 acagcccatg tctggaggcc ttttcgcggg gaaaggctgc ggaacgcgat gagagcctgg
    11041 taaccgggtt gctcaaccgt ggcataactg gtagcctgag atgagtacga acgaacggac
    11101 ggtgctctcc tggccgcaag attacttccg ttcgatgtcg cagaattgtg cgccgctgcc
    11161 gcggccaccc aggcaattcc accgtgttgt gccggcgctg tttcgtaccc aagctctatc
    11221 cggtccaaca gactgtcagc cattacggaa taccagggag cgcgcgacga caaccgttcc
    11281 aaaccgagga gcaatgggac cgctttatgc gtagcatccg gtatgtcctg cctcgtggat
    11341 acgccccgga acgtagtata ccggtcggtg tacatccgtg taacccagtc tgtggagcgt
    11401 tcaataacgg ggtcgaggga gtcgtctgcc gtggcggtgc ggtaccgtaa gagatactcg
    11461 tacgaaatca tggagtagcc gttgcacggc ccgcaacctt cgaaaatctg cccgttttcg
    11521 agaaacacgt ccggggctac acgttcccac acggcctgcg cgtccgcgag gtaccgttcg
    11581 tcaccggtag cgcgccagca catcgccatc accgcacacc aggtcacacc ccggttacaa
    11641 cggtgttcgc acggagtctc tcgaaggaac tccatcgcgc gcaagagcgt gtcccggtat
    11701 cgggaccgtt cctcggaagt cagcgcctct ctgacacaca gaaatgcggt gagaagacac
    11761 tccagtcgcc atgccatttc gtgggtgccg tacacggagc agaatcgtat cggggtgaag
    11821 acgaattcgc ccgaagggac ctgga
//
LOCUS       DAOTNG010000062        11835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5152_length_11835_cov_5.335059, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000062 DAOTNG010000000
VERSION     DAOTNG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5152_length_11835_cov_5.335059"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="P5179_04535"
     CDS             <1..1861
                     /locus_tag="P5179_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS94520.1"
                     /translation="LHFVNERKHDRLDFTAQPKVAAAFGYRDDAAALGVEHFMRDYYL
                     AAREIVRISDAVCHQALRRRRGIQTVTDIVRRRVLPDGAVYVRGKILLPKRRKGFFAT
                     NPRRMLSLFADMQRFGAIMSETASWAIYDELALVDEDFRTDPENAAVFLSILSEPAHL
                     GAVLRRMHWTGLLGAFIPEFGRLTCQVQHDYYHAYTADEHSIIVIDRLSELALTDDDS
                     RVATVYRSLSRKHLLHLAALLHDAGKSGGHGHAERGAEMSITITRRLGLEKADQDLVA
                     FLVAHHLRLSHLSQRRDIDDLAMIRAEAERFSDTDTLDMLYVLTRADMNATQARPLSR
                     WKCQLLDTLHQRLREVVETRGLGPEFAALRTLESPQKFRNLLAERIGEEAAERHLSGM
                     PRRYSLMHSLDEAATHALRADSLKNEPVLVDVAPAGNHARVIVYTRDRRYLLSDICGV
                     LAVNNLNILYADAYTRTDGIIVDVFGVEVVGESDIRDEAFVSTLRDTFRSVWTGQAVV
                     AELIARHRRRWARIKPKAGMAPSHVVIDNTVSETNTVIDVFAIDRIGLLYDISRAISE
                     NGLDINMARIGTDGDRVADAFYVVTSSGEKLIDAAESEKVRESVLRVIGNERT"
     gene            2271..3518
                     /gene="rho"
                     /locus_tag="P5179_04540"
     CDS             2271..3518
                     /gene="rho"
                     /locus_tag="P5179_04540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRS94521.1"
                     /translation="MNIIELKKKTIAELHELAHALGIPEYTSYRKQELIFKILEAETA
                     KSGFIFAEGVLEILEQGNGFLRSPDYNYLPGPDDIYVSQSQIKRFGLRTGDTVSGQIR
                     PPKENERHFALLRVEAVNFENPDLAKEKTLFDNLTPLFPNRRINLEHYPNELSTRIVN
                     LLAPIGMGQRGLIVSPPKAGKTMLLQKIANAITHNHPDIKLIVLLIDERPEEVTDMQR
                     SVDGEVISSTFDEPPNRHVQVSDMVIEKARRLVEYGHDVVILLDSITRLARAHNTVVP
                     HSGKILSGGVDANALHGPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNMELVLDRKLSDRRIFPAIDVNKSGTRREELLLTEFELHRVWILRKFLAQMSSAEA
                     MEFLIDRMHGTKTNHEFLDMMNG"
     gene            3649..3858
                     /gene="rpmE"
                     /locus_tag="P5179_04545"
     CDS             3649..3858
                     /gene="rpmE"
                     /locus_tag="P5179_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRS94522.1"
                     /translation="MKSGIHPEYHATTIACACGNVVQTSSTKKNLRVEICGACHPFFT
                     GKQKLIDTAGRVDAFRRRYNRSEAK"
     gene            3967..4857
                     /locus_tag="P5179_04550"
     CDS             3967..4857
                     /locus_tag="P5179_04550"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRS94523.1"
                     /translation="MIRSPRSIAIALRTPRGIAVETRDYVNLVARTRWLSLPVLRGVV
                     SFFEMLGIGLNALTYSANVATSDGCDTPVKDRISPAEALLFLISFGLGVGLFVFLPMA
                     LATLAGLTNRPVAYNLFAGTVRILVFLSYLWAIGKIADVKRVFEYHGAEHKAVAAFES
                     GLDSELTAQTARGFPRFHPRCGTSFVFLVVAVAIAFYAITDSLYALYAGNAPDLETRF
                     LLHLACLPVVAGLGYEVLKATGKSRNSRRARYISKPGLLLQRLTTREPNDDQLSVAIA
                     AVRAAVGLPQETGGPVSCAV"
     gene            4883..5962
                     /gene="prfA"
                     /locus_tag="P5179_04555"
     CDS             4883..5962
                     /gene="prfA"
                     /locus_tag="P5179_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRS94524.1"
                     /translation="MLDRLAAIETRFTEIERLLSDPASTTDIAQYQKLAKEHRQLAPI
                     VETGRRYADVLAQLAEARRIVEEENDRDLIAMAKEEIVVLEDRAASLEETLKEQLIPK
                     DPNDDRNTILEIRGGTGGDEAALFAADLFRMYSRYAESKGWSLEVMSTSEASAGGLKE
                     VIAQISGEGAYGRLKYESGVHRVQRVPATEAQGRIHTSTASVAVLPEADDVEVEIDPA
                     DLRIDVFRSSGPGGQSVNTADSAIRITHLPTGLVVQCQDERSQLKNKAKAMKVLRARL
                     LDRAIIQQQSEEAAARRAQVGTGERSEKIRTYNFPQSRVTDHRIKLTVHNLSEILDGN
                     LDELIEALRMADRANQLQHPSAEDD"
     gene            6009..>6343
                     /locus_tag="P5179_04560"
     CDS             6009..>6343
                     /locus_tag="P5179_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRS94525.1"
                     /translation="MIDLRLIREQTDFVKREIAKLFTDAPIDEILSLDEARRNLIREV
                     EELKARRNAASKAISAVKDSAERQSRILEMREVGDRIAVLDEQVRAVEERLNARLLEV
                     PNLPHPDVPV"
     assembly_gap    6344..6443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6444..7435
                     /gene="serS"
                     /locus_tag="P5179_04565"
     CDS             <6444..7435
                     /gene="serS"
                     /locus_tag="P5179_04565"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRS94526.1"
                     /translation="LNARLLEVPNLPHPDVPVGRDESENVVTAMEGDLPTFTFEPRPH
                     WEIGPDLGILDFERGVKISGSRFYVLVGDGARLQRALITWMLDLHRTRHGYLEVYPPA
                     VVRHDCLIGTGQLPKFGENLYHDAEEDFWLVPTAEVPVTNLYAGEVLEKSQLPLYHVA
                     YTPCFRREKMSAGKDVRGIKRGHEFDKVEMVKFVEPATSDAELTSLVEHARDVCRGLG
                     LPHRIVQMCTGDLSFTAAMKYDVEVWAAGCGEWLEVSSCSNFRDFQARRANIRYRPAP
                     NARPEYVHTLNGSGLALPRVMIAIMENYQLEDGSIVIPEVLRPYLGGQDTISA"
     gene            complement(7496..8467)
                     /locus_tag="P5179_04570"
     CDS             complement(7496..8467)
                     /locus_tag="P5179_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94527.1"
                     /translation="MARRLWLSVALFAVVAQSGCILNPVKPEKKNVPELFLQGFLVAG
                     GPIDNIILRKTIPPEMYYDFMPLGRYAVSGAEVVITIDGAPHPLAELHPGTYGDPNLI
                     ARSGATCEIEVRFPANHEFTGRRLYGATTIPEKPRASVAIHPYYVQRGAHLNGNSLTG
                     LVFPRELSHPDSFGTTAEKEPFRLSWPLSASAAGYMVVSTAVDTQGTGLLRQRDYEEY
                     LDGVFRDPVKRQGKRTAGFYVLPDSSQSTVYWLLFSYQGWHDVSVVACDTGYWDYMVT
                     VLNDAGTGADSDAGVKLNVQGGLGIFCSYAADTTRVYIQPEWLPSTR"
     gene            complement(8467..10722)
                     /locus_tag="P5179_04575"
     CDS             complement(8467..10722)
                     /locus_tag="P5179_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94528.1"
                     /translation="MTSGQTVAATSISGYVRERGSRESIPYVSVYIEGLRLGGMTNAA
                     GYYVINGVPPGRHVIVAAMVGYNESRQTLVVATEPLVHNIELSTEAIEVAAVEVQAER
                     ARAPSLEIIPSRETLRGVDLKIAPAAVEADPIRTLHTLPGVVQLSDFNTGLYVRGGTP
                     DQNLILVDGSELYNVSHMFGLFSTFPADAIKTTELLMGGYPAQYGGRLSSVLNVITDE
                     GNKERFKGSGGVSLLSSRMTLQGPFAKGSFLVSGRRTYLEPVLWAASRSSDAFDGMGY
                     FFYDFQGKVHQILSHSDQFAIAGYMGKDAFKYTNDFMDTRFDWGNKSASLSWNHLFSG
                     ELYSHLQANLSRFVSHTTFKIEDFGATDHNELFDTDLRGDLTWFANERHTFETGVHVK
                     RNTMRFGQEYAGQRYEAFNLIAYVPSAYLQDSWRPWPFLTLQPGLRATLFRSRLRDTT
                     GSATYSAFEPRFTVRYQLGEQTFLKAATGRYSQFIFRVPREYEGISMMSDIWFTCDDT
                     PGGYPQRAWHYVLGLETKVRSDVDLSLEAYYKDYAGLVEFNASQTAPQSVEDALLRGD
                     GYAYGLDVNVKKRAGRNTGWLSYSMSWTVRTIDGLNLDENGNPQPFYPKFDARHHLDA
                     VYSFDLSRHWTLNSRMSLSSGQGYTKVLGRYVVEDPLFTQGLYYRGQKNGARLPYYMR
                     LDVGAKGSYRFWGVRWMPFFQIVNVLNRANEFNRYYDSGDATKNPPETGRERAIPQLP
                     FLPTFGVDVEF"
     gene            complement(10944..>11835)
                     /locus_tag="P5179_04580"
     CDS             complement(10944..>11835)
                     /locus_tag="P5179_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRS94529.1"
                     /translation="IKRFWRYLIARYGAYPVVWCAAGEGTMPWYLSKTPREDAQKQKS
                     GWTDVCRYIRSTDGFGNVLTIHPTRYGHEQVEDRAVLDLNMLQTGHSGYESVGPTIES
                     VRTAYALNPPMPVLDSEVNYEGILGKSWQDVQRLCFWHSILNGACGHTYGANGIWQCS
                     TKAEPYGASPHGRSWGNTPWQEAMKLPGSTQLGLARKFLLRIDWPQMEPHPEWTQDGA
                     SAAGIPKRLRVIYSPMVWDPPAIKELEPDVKYHARFFDPVTGAEYPIGPVDPAPDRTW
                     TPPVFPEMHDWVIVLKAVTG"
BASE COUNT         2503 a         3375 c         3415 g         2442 t
ORIGIN      
        1 gctccacttt gtgaacgaga ggaagcacga ccggctcgac ttcacagctc agcccaaggt
       61 ggctgcagca ttcggatacc gggacgacgc tgccgcactg ggagtggagc atttcatgcg
      121 ggattactat ctcgcggcgc gggagatcgt acggatatcg gatgccgtgt gccatcaggc
      181 actccgccgg cgacgcggga tccagactgt gaccgatatt gtccggcggc gtgtactccc
      241 ggacggggcg gtctacgttc gcggaaagat cctcctcccc aagcgcagga aaggcttttt
      301 cgccaccaat ccgcgccgga tgttgagcct gttcgccgac atgcagcgct ttggcgccat
      361 catgagcgag acggcgtcgt gggcgattta cgacgaactc gcactggtcg atgaggactt
      421 ccgaacggac ccggaaaatg cggctgtctt tctgtcaatc ctgagtgaac cggcgcatct
      481 gggggcggta ctccgccgta tgcactggac aggtttgctg ggtgcgttca ttcctgagtt
      541 cgggcgcttg acctgtcagg tgcaacatga ctactaccac gcctacacgg cggacgagca
      601 ctccatcatc gtgattgacc gcttgagcga gctcgcgttg accgacgacg attcgcgagt
      661 ggcaacagtg taccgttctc tctcgaggaa gcatctcctc catcttgcgg cgcttctgca
      721 cgacgcaggc aagtccggcg ggcacgggca cgcggagcgc ggcgcagaga tgtcgattac
      781 catcactcga agactcggtc tggaaaaagc cgaccaggat ctcgttgcct ttctcgtggc
      841 acaccatctt cggttaagcc acctgtcgca acgccgtgac atcgatgatc tcgcgatgat
      901 cagggctgag gcggaacgtt tcagcgacac tgacacgctt gacatgctgt acgtgcttac
      961 gagggccgat atgaacgcaa cgcaggcgcg accgctttcg cgatggaaat gtcagcttct
     1021 ggatacgctt catcagcgtc ttcgcgaggt tgtggaaacg cgcgggctcg ggcctgagtt
     1081 cgctgccctc cgcacactgg agagccctca gaagtttcgc aaccttcttg ccgaaagaat
     1141 cggcgaagaa gcggcggaac gccatctttc gggtatgccg cgacgctatt cgctgatgca
     1201 ctccctcgac gaagcagcta ctcacgcgct gcgagcggat tccctgaaaa atgaacctgt
     1261 gctggtcgac gtagcgcctg caggtaacca cgcacgcgtg atcgtgtata cgagagaccg
     1321 caggtacctc ctcagcgaca tctgcggagt actcgccgtc aacaacctga atatcctcta
     1381 cgctgatgcg tatacgcgca cggacggaat catcgttgac gtattcggag tagaggtggt
     1441 gggagagagc gacataaggg acgaggcgtt cgtgagtact cttcgcgaca cgttccggtc
     1501 tgtgtggaca gggcaggccg tggttgcaga actcatagcg cggcatcgaa gaagatgggc
     1561 tcggatcaag cccaaagccg gcatggcacc ctctcacgtg gtaattgaca acacggtaag
     1621 tgaaaccaat actgtgattg atgtgtttgc aatagacagg atcgggctgc tttacgacat
     1681 cagcagagcg atctcggaga acgggctcga tatcaacatg gcccggattg gaacagacgg
     1741 ggatcgcgtg gcagacgcgt tctatgtagt aacgagcagc ggggagaaac tgatcgacgc
     1801 cgcagaaagt gagaaggtcc gcgagtctgt tcttcgcgtg atcgggaatg agcggacttg
     1861 acacttccgg ctcaaggccc tgtgttagag acacgcaact accccgcaat tttccgtgaa
     1921 ctttcttgct gttcgcctgt caagtgacct cttgacgaat gcccttgaga taccatctgg
     1981 aaggacatat ggtagcggcg caactcagat tgtgccggca ggttttgttt cgttcgctca
     2041 atgcgcgtcg cgctctcttt cgcgagcacg aataactggc gaaaacgact aaaagacctg
     2101 acttccattc gataatcgtt tctctggctt cccgaacgca ctaacagaac tcgtcccggt
     2161 acgtcgcgta tccgtatccg gaacgagctt atatggaatg cccctttatg ggcccgcgac
     2221 ctacacgcaa agcccgaggt tccaccaaat ctcctgccgg aggcacaccc ttgaacatca
     2281 tcgaattgaa gaagaaaacc atcgccgagc tccatgaact ggcgcacgcg ctcggcatac
     2341 ccgagtacac cagctaccgc aaacaggaac ttattttcaa gatcttggag gcggaaaccg
     2401 cgaaaagtgg cttcatattc gccgagggtg tgctcgagat tctggagcaa gggaacggct
     2461 tccttcggtc gcctgactac aactacctgc cgggcccgga cgatatttac gtcagccagt
     2521 ctcagatcaa acgttttggt cttcgaacgg gcgacacggt cagcggccaa atccgtccgc
     2581 cgaaagaaaa cgagcggcat tttgcgttgc ttcgtgtgga ggcggtcaat tttgagaacc
     2641 ccgatctcgc caaagagaag acgctttttg ataaccttac gccgctgttc ccgaaccgcc
     2701 gcatcaatct ggagcattac ccgaacgagc tttcgacgcg catcgtaaac cttcttgcgc
     2761 cgatcggtat gggtcagcgg ggcctcatcg tgtcgccgcc gaaagccggc aagacgatgc
     2821 ttctgcagaa aatcgcaaac gcgatcacgc acaatcaccc tgatatcaaa ctgatcgttc
     2881 tgcttatcga tgaacggccg gaagaagtga ctgacatgca gcggtcggtg gatggcgagg
     2941 tgatcagctc cacgttcgac gagccgccga accgccacgt gcaggtttcg gacatggtta
     3001 tcgagaaggc gcggcggctg gttgaatacg ggcacgatgt ggtgattttg ctggacagca
     3061 tcaccagact tgcccgcgcc cacaacacgg tggtacctca ctcggggaaa attctctccg
     3121 gcggtgtgga tgcgaacgca ttgcatggcc cgaagcggtt tttcggagct gcgcgaaaca
     3181 tcgaagaggg cggttcgctt acgatcatcg ccaccgcgct cattgatacg ggcagccgta
     3241 tggacgaggt gattttcgag gaattcaagg gaacgggcaa catggaactc gtgctcgacc
     3301 gtaagctttc ggatcggcgc atcttccccg cgattgatgt gaacaaatca gggacgcgtc
     3361 gcgaagagct tctcctcacc gaattcgaac tgcatcgtgt gtggatactt cggaagttcc
     3421 tggcgcagat gagttccgcg gaggcgatgg aattccttat cgaccggatg cacggcacaa
     3481 agacgaatca cgaattcctc gacatgatga acggctgatc gacgcgaccg ggcgcccggg
     3541 tcaatcgcca gaatttggcc ggaaggtgcg tcgaaagtta tatttagaaa ttcagtttgc
     3601 tttttcagac aaacgtgcgg accccttaga acgtgtggag cacacctgat gaaatccgga
     3661 atccatcctg aatatcacgc cacgaccatc gcctgcgcgt gtggtaacgt cgttcagacg
     3721 tcctcaacga agaagaacct tcgcgtcgaa atctgtggtg cgtgccatcc cttcttcacc
     3781 ggcaagcaga aactgataga cactgctgga agagtagatg ccttccgacg tcggtacaac
     3841 cgttcggaag cgaagtaacc gccgggcaga atggaatctt ctcctcccgc agatcgcacg
     3901 gactcgatta cagacagaac cagactcgat ctttcggtcg gcggccaagc gctggtcgaa
     3961 ggggtcatga tacgttctcc ccggtccatt gccatcgcct tgcgcacgcc tcgcggaatc
     4021 gctgttgaaa cccgggacta cgtgaatctg gtggcgcgca cacgctggtt gtcgctgccg
     4081 gttttgcgtg gcgtagtttc tttttttgag atgcttggca tcggcctgaa tgctctgacg
     4141 tacagcgcga acgttgcaac ctccgatgga tgcgatactc cggtgaagga tcggatatcc
     4201 ccggcggagg cgcttctttt tttgatatcg ttcgggctcg gggttggcct tttcgtgttc
     4261 cttcctatgg cgttggccac tctcgccggt ctgacgaacc gtccggtggc gtacaacctt
     4321 ttcgccggca ccgtgcgcat tctcgttttt ctttcctacc tctgggcgat tggaaaaatc
     4381 gcggacgtga agcgcgtgtt tgagtaccac ggcgcggaac acaaggcagt ggcagcgttc
     4441 gaatcaggat tggactcgga actcacggcg cagacggcga gagggttccc acgtttccat
     4501 ccccgttgcg gaaccagttt cgttttcctc gtcgtggcgg tggcgatcgc gttctacgcg
     4561 atcactgact cgttatacgc gctttatgcg ggtaacgcgc cggacctcga gacgcgcttt
     4621 ctccttcacc tcgcctgcct cccggtcgtt gcgggtcttg gctatgaggt tttgaaggca
     4681 accggaaaat cccggaattc tcgccgggcg cgttacattt cgaaaccggg actcttgttg
     4741 cagcgcctca cgacacgcga accgaacgat gatcagcttt ccgtcgctat cgccgctgtg
     4801 cgcgcagccg tcggtcttcc acaggaaacc ggtggcccgg taagttgcgc cgtctgaatg
     4861 atatagagga aggaacacgg aaatgttgga tcgcctcgcc gccattgaga ctcgattcac
     4921 cgagatcgag cggcttctct ccgatccggc gtcgaccacg gatatcgccc agtaccaaaa
     4981 actcgcgaaa gaacaccggc agcttgcacc gattgtggaa acgggccgac ggtacgcgga
     5041 cgtactcgcc cagcttgcgg aagcccggcg aattgtggaa gaggaaaacg acagggacct
     5101 gatcgcgatg gcgaaggagg agatcgtcgt tctggaagac cgcgcggcaa gtctcgaaga
     5161 gacgctgaag gaacaactga ttcccaagga tcccaacgac gacagaaata cgatccttga
     5221 gatccgcggc ggtaccggtg gcgacgaggc agcgttgttt gcggccgacc tttttcgcat
     5281 gtattcccgg tatgcggaat ccaaagggtg gtcgctggaa gtcatgagca cgagcgaagc
     5341 ctctgcgggc ggacttaagg aagtcatcgc ccagatttcg ggagaaggcg cgtacggccg
     5401 cctgaaatac gaaagcggcg tccacagggt tcagcgtgta cctgcaacgg aggcccaggg
     5461 tagaatacac accagtactg cttccgtcgc cgtactgccc gaagccgacg acgtggaagt
     5521 ggagatcgac ccggcagatc tgaggataga cgtgttccgc tccagcggcc ccggggggca
     5581 gagcgtgaac accgcagatt cggccatcag gattacccat ttgcccactg ggcttgtggt
     5641 ccaatgtcag gacgagcgtt cgcagttgaa aaacaaagcg aaggctatga aagtgctccg
     5701 cgcgcgtctt cttgaccgtg cgatcatcca gcagcaatcc gaagaggccg ccgcgcggag
     5761 ggcgcaggtt ggtacgggtg agcggagcga aaaaatacgg acctacaact tcccgcagag
     5821 ccgcgtcacc gaccaccgta tcaagttgac ggtgcacaac ctgagcgaga tacttgatgg
     5881 gaaccttgac gaactcatag aagcgttacg gatggcggac cgcgcaaacc agcttcaaca
     5941 tccgtcggcg gaagacgact gattgtgctg ccggtggatt tcgcaccgca ttgcggagag
     6001 acgttgccat gattgatctg agattgatca gagaacagac ggatttcgtc aaacgggaaa
     6061 tcgcgaagct tttcactgac gcgccgatag acgaaatcct ctcccttgac gaagcgcgaa
     6121 ggaacctgat tcgcgaggtc gaggaactca aggcgcgccg caatgctgct tcaaaggcga
     6181 tcagcgccgt caaggactca gcggaacggc agtcacggat tctcgagatg cgtgaagtcg
     6241 gcgatcgcat tgctgttctc gatgagcagg tgcgcgcagt ggaagagcga ctcaacgccc
     6301 gcctgctgga ggtgcccaac cttcctcatc cggacgtccc ggtnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnngactcaa cgcccgcctg ctggaggtgc ccaaccttcc
     6481 tcatccggac gtcccggtgg ggagagacga atcagagaac gtcgtgactg ccatggaggg
     6541 tgatcttccc acgttcacct tcgaaccccg tccccattgg gaaatcgggc cggacctcgg
     6601 gattctcgat tttgagcgcg gcgtgaaaat aagtggctca aggttctatg ttctcgtggg
     6661 agatggcgca cgcctccaac gcgcactcat cacatggatg ctcgacctcc accggacgag
     6721 gcacgggtac ctggaagtct accctcccgc cgtggtacga cacgattgtc tgataggcac
     6781 cggccagctt ccgaaattcg gtgagaatct ttaccacgac gccgaggaag acttctggct
     6841 ggttcccacg gcggaggtgc ctgtcacgaa cctgtacgct ggcgaagtgc tggaaaaatc
     6901 ccagctaccg ctctatcacg tagcgtacac cccctgcttc cgtcgggaga aaatgtccgc
     6961 gggaaaagac gtccgcggta tcaagcgcgg ccacgagttt gacaaggtcg agatggtgaa
     7021 gttcgtggag ccagcaactt ccgatgcaga gctgacgtct cttgtcgaac atgcccggga
     7081 tgtgtgcaga gggctcgggc ttccgcatcg cattgtgcaa atgtgcaccg gcgatctgag
     7141 tttcaccgcg gcaatgaaat acgatgtgga agtgtgggcg gcaggttgcg gggaatggct
     7201 ggaggtcagc tcctgcagca atttccgtga ctttcaggcg cggcgtgcga atatccggta
     7261 ccgcccggca ccgaacgcca ggccggaata cgttcacacg ctgaacggct cgggactcgc
     7321 gttaccgcgc gtgatgattg cgataatgga aaactaccag cttgaggatg ggagcatcgt
     7381 gattccggaa gtgctcaggc cgtacctcgg cgggcaggac acaatctcgg cgtagagggc
     7441 ataccatcgg ggttcacacg gggcgccttg cgggcgccct tttgtttttc gtgcgtcagc
     7501 gggttgacgg gagccactca ggctggatgt acacacgcgt cgtatcggcg gcgtacgaac
     7561 agaaaatccc gagccctccc tgaacgttca gcttcacccc ggcatccgaa tcagcaccgg
     7621 ttccggcgtc attgagcact gtcaccatgt agtcccagta cccggtatcg caggccacta
     7681 ctgacacatc atgccagccc tggtaactga acagcagcca gtacacggtg ctctgtgagg
     7741 aatcgggaag aacgtagaat cccgccgtcc gcttgccctg gcgcttcaca gggtcccgga
     7801 acaccccgtc aagatactcc tcgtaatctc gttgccgcag aagcccggta ccctgcgtat
     7861 ccacggcagt tgagaccacc atgtacccgg ctgccgaagc acttagcggc cacgaaagcc
     7921 ggaacggttc cttttccgca gtggtgccga aggaatccgg gtgagagagt tcccgcggaa
     7981 acacgagccc ggtgagggaa ttgccgttga gatgggcacc ccgttgtacg tagtacgggt
     8041 gaattgctac tgacgcacgc ggcttttctg gaatcgtcgt ggcgccgtac aacctccggc
     8101 cggtgaattc gtggtttgcg gggaaacgca cctcgatttc acacgtagcc cctgagcgag
     8161 caatcaggtt tgggtcaccg tacgttccgg gatggagttc tgcgagcggg tgcggggcgc
     8221 catcgatggt gatcacgacc tcggcacccg acactgcgta ccttccgagc ggcatgaagt
     8281 cgtaatacat ctccggcggg attgttttcc ggaggatgat gttgtcgatc gggccgccgg
     8341 cgaccagaaa cccttgcagg aagagctcgg gaacgttctt tttttcgggt ttgaccgggt
     8401 tgagaatgca ccctgattgc gccacaaccg cgaacaacgc aaccgaaagc cagagtctcc
     8461 ttgccatcag aactccacat ccacgccgaa ggttggaagg aacggcaact gggggatcgc
     8521 tcgttcgcgg ccggtttccg gtgggttctt cgtcgcgtcg ccgctgtcat agtatctgtt
     8581 gaactcattc gcgcgattga gaacgttcac aatctggaag aacggcatcc acctgactcc
     8641 ccagaatcgg taactaccct tcgcacctac gtccagacgc atgtagtagg gcaaacgggc
     8701 cccatttttc tgtccccggt agtagagacc ctgggtgaaa agaggatcct ccaccacata
     8761 ccgcccgagt acttttgtat acccctgccc tgacgacaat gacatgcggc tgttgagggt
     8821 ccaatggcgc gatagatcaa agctgtacac cgcgtccaga tgatgtcttg cgtcgaattt
     8881 cgggtagaac ggttgcgggt tcccgttctc gtcgaggttc aggccgtcga tcgttcgaac
     8941 ggtccaactc atcgagtacg acagccatcc cgtgtttcgg ccggctcgtt tctttacgtt
     9001 aacatccaga ccgtatgcat atccgtcacc cctcaggagt gcgtcttcga cgctctgcgg
     9061 ggcggtctgt gacgcattga actccacgag ccctgcgtaa tccttgtaat acgcttcaag
     9121 cgacaaatcg acatcgctcc gtaccttcgt ttccagcccc agcacgtagt gccacgcgcg
     9181 ctggggatac cctcccggcg tatcgtcaca tgtgaaccag atgtcggaca tcatcgagat
     9241 gccctcatat tctctgggca cgcggaaaat gaactggctg tatcgcccgg tggccgcctt
     9301 cagaaaggtc tgttccccca actggtatcg tacagtgaac cggggttcga atgctgaata
     9361 ggttgcagac ccggttgtgt ctcgaagccg gctccggaag agcgtcgccc taagaccggg
     9421 ttgcagcgtg aggaatggcc acggtctcca gctatcctgc agatatgcgc tgggaacgta
     9481 cgcgataaga ttgaatgctt cgtaacgttg tcccgcgtat tcctgcccga accgcatggt
     9541 gtttcgtttc acatgaacgc cggtttcgaa cgtatggcgc tcgttggcga accaggtaag
     9601 atcacccctg agatccgtgt cgaagagctc gttatgatcc gtggcgccga aatcctcgat
     9661 cttgaacgta gtgtgcgaaa cgaaccggct aagattcgcc tgcaaatggg agtagagttc
     9721 accggaaaac aggtgattcc agctcagcga ggccgatttg ttgccccagt cgaaacgggt
     9781 gtccatgaaa tcgttggtgt atttgaacgc atctttgccc atgtatccgg cgatggcgaa
     9841 ctgatcggag tgcgaaagaa tctgatgtac cttgccctgg aagtcgtaga agaaataccc
     9901 catgccgtcg aacgcatcgc tcgagcggct cgcagcccac aacacgggct ccaggtacgt
     9961 ccgccgtccc gacacgagaa acgagccttt cgcgaacggg ccctgcaatg tcatgcggga
    10021 gctcagcagg ctaaccccgc ctgagccttt gaatcgctcc ttgtttcctt cgtccgtgat
    10081 gacgttgagc acggaggaga gccggccacc gtattgtgcc gggtatcccc ccatgagcaa
    10141 ttcggtggtt ttgatcgcat ccgcgggaaa cgtggagaac agaccgaaca tgtgcgacac
    10201 gttgtacagt tcggaaccat ccaccaggat caggttttga tccggcgttc ctccacgaac
    10261 gtacaacccg gtgttgaaat cggacagttg caccacaccc gggagcgtat gcagtgtccg
    10321 aatcgggtcg gcctccactg ccgccggagc aatcttcaaa tccacaccgc gaagcgtttc
    10381 tctgctggga atgatttcga gcgaaggggc acgggcgcgt tccgcctgca cttccactgc
    10441 cgcaacctca atcgcttccg tggacagctc aatgttgtgc acgagcggtt ccgtggcgac
    10501 gaccaacgtc tgccgcgatt cgttgtaacc gaccatggcg gcgacgatca catggcggcc
    10561 cggcgggacg ccattgatga cgtagtaacc tgccgcgttt gtcattccgc ccagacgcaa
    10621 accctctatg tagacgctga cgtaagggat gctttcccgg ctgccgcgtt cacggacgta
    10681 cccgcttatc gacgttgcgg caaccgtttg cccgcttgtc accaccaatg ccaccagccc
    10741 gacgattgtg ggaaacgcgg attctcggca gccgctttga ggtccttgac ttttcaggtt
    10801 catgtcggca ggagcaaccc ggttggacac tcggccgaaa aagagttttt tggaccagga
    10861 ccaggcattc gcccgaccat tatggaggtc atgcgcggct cgtcttcgcg gtgcctggag
    10921 cccaataatt acttgcgccc aggtcatccg gtgaccgctt tcagaacgat tacccagtcg
    10981 tgcatttcgg ggaagacagg gggcgtccat gttctgtcgg gagcgggatc caccggaccg
    11041 ataggatact ccgcacctgt aaccgggtcg aagaagcgtg catggtattt cacatcgggt
    11101 tccaattctt tgatggctgg aggatcccag accatcggtg aatagatcac gcgcaaccgc
    11161 ttgggaatgc cggctgcact ggccccatct tgcgtccact ccgggtgggg ttccatctgg
    11221 ggccaatcga tccgcaagag gaactttcgc gccagcccga gctgggtgga cccaggaagc
    11281 ttcatcgcct cctgccaggg tgtgttgccc caggaacgtc cgtgtggcga cgcaccgtac
    11341 ggctccgctt ttgtactgca ttgccagatg ccgtttgcgc cgtacgtgtg cccgcacgcg
    11401 ccgttcagaa tcgaatgcca gaaacagagc cgctggacat cctgccagct cttgccgaga
    11461 atcccctcgt aattcacttc gctatccaga acgggcatgg gtggattgag cgcgtacgcg
    11521 gtgcgcacac tttcgatcgt cggacctacg ctttcgtaac cgctatgccc ggtctgcaac
    11581 atgttcaggt caagtaccgc tctgtcttcc acctgctcat gcccgtaccg ggtcgggtga
    11641 atggtcagca cgtttccaaa cccatcggtc gagcgaatat aacggcacac gtccgtccag
    11701 cctgatttct gcttctgagc gtcctctctg ggcgttttcg acagatacca cggcatggtt
    11761 ccctcgccgg cggcgcacca tacaacggga tacgcgccgt accgcgcgat gaggtaccgc
    11821 cagaaacgtt tgatt
//
LOCUS       DAOTNG010000063        11750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5202_length_11750_cov_5.309705, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000063 DAOTNG010000000
VERSION     DAOTNG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_11750_cov_5.309705"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5179_04585"
     CDS             <1..229
                     /locus_tag="P5179_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94530.1"
                     /translation="RIARTMAQTWRGPFAINRLLFRLNGTTVVGAGEGGRGRCGKTFL
                     SALMEDFSMGAMRSFASSTSDTAAAQNDARF"
     gene            322..477
                     /locus_tag="P5179_04590"
     CDS             322..477
                     /locus_tag="P5179_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94531.1"
                     /translation="MRTSLIPSLAAAAASHIRRTYAVSARDQIVNSLVTILLLVLVLG
                     LLVPVSR"
     gene            827..2518
                     /gene="ilvB"
                     /locus_tag="P5179_04595"
     CDS             827..2518
                     /gene="ilvB"
                     /locus_tag="P5179_04595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRS94532.1"
                     /translation="MEGKECTGAEILVECIIREGVDLMFGYPGGVVLKVFDELYSRRD
                     QIRVVLPRHEQAGVHAADGWAKVTGKVGTVFVTSGPGATNAVTGIATAQMDSVPLVVF
                     TGQVSRELIGSDAFQETDAIGVTRPIVKHSYMIERPQDIARIVRQAYYIASTGRPGVV
                     LVDVPKNVTFEKAVFEWPETVSIRSYKPTTEGHPKSIQRLADAIAESKRPYLYVGGGV
                     VMANASDHLLELVNKTNIPVTTTLMALGAFPGTHPLFAGMPGMHGSKLANICFQECDL
                     VIAVGARFDDRVTGKLSAFAPKAKIAHVDIDPSSIKKTVKVDYPVVGDAHWVLRELNK
                     VVKPRQPNEWNRTFAEMKETHWFRYRPSTEVMRPQYVIEKIYEMTKGDAIISTEVGQI
                     QMWAAQFYKFDKPRRWLTSGGLGTMGYGFPAAIGAQFAAPDAMVIDIAGDGSIQMNIQ
                     ELTTVVENRLPVKVVILNNGYLGMVRQWQEFFYDRHYSSSVIGGLPDFAKVADAFGAK
                     GLTARTHDDVVPVLEEGLAHPGPVFMDFHINPEENVFPMVPAGKALDEMQEEEWA"
     gene            2519..3043
                     /gene="ilvN"
                     /locus_tag="P5179_04600"
     CDS             2519..3043
                     /gene="ilvN"
                     /locus_tag="P5179_04600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRS94533.1"
                     /translation="MNRRHIISVLVENHFGVLARISSLIAGRGFNIDSLTVGETEDPT
                     VSRMTIVTRGDEQIIEQITKQLNRLIDVIKVTDMTECAHVERELVLIKVAAPTAQTRT
                     EVVQVVDVFRARVVDMSTATLTIEITGDSEKVDAFIEMIEPFGIKEMARTGSVAMGRE
                     RNGRASRGAETKVA"
     gene            3145..4221
                     /gene="ilvC"
                     /locus_tag="P5179_04605"
     CDS             3145..4221
                     /gene="ilvC"
                     /locus_tag="P5179_04605"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRS94534.1"
                     /translation="MSTQKEETTALATETRARMYYDDDANLDLLKDKTVAVIGCGSQG
                     HAHALNLQDSGVKVVVGLRQGSKTWAKAEAAGLKVLETAEAAKQGDIIMMLVPDTAAS
                     EIYERYIAANLTPGKALAFAHGFNIHFKTIVPPTNVDVFMVAPKGPGHLVRATYTRGS
                     GVPNLIAIYQNPSGKAKDIALAYAKGIGGTRVGVIETTFKEETETDLFGEQVVLCGGL
                     SKLIQTAFETLVEAGYQPEIAYFECLHEVKLITDLIYVGGISTMRYSISDTAEWGDYV
                     TGKRIITEETRNEMRRVLAEIQSGKFAKEWVAEYKAGLPNFKRMREEQSQHLIEQTGA
                     PLREMMKGALKLEGGGTEKFLSDK"
     gene            4474..6036
                     /locus_tag="P5179_04610"
     CDS             4474..6036
                     /locus_tag="P5179_04610"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRS94535.1"
                     /translation="MSDDSSVVRIFDTTLRDGEQSPGASLSVQEKIDIAHQLKRLGVD
                     IIEAGFPVSSPAQFEAVERISEEVDGPTIAALARAVPLDIETAARAVKPAKKKRIHTF
                     IATSPIHMERKLKKTPAEVLTLTGEAVRLAKSFCDDVEFSPEDAGRSEWPFLVEVLTA
                     AIEAGATTLNIPDTVGYMMPEEYGRMIAHLIAHVPGSENVIYSTHCHNDLGLAVANSI
                     AGVVNGARQVECTINGLGERAGNAAMEEFVMALRTRQDVFKLRTNIVTTEITRTSRMV
                     ARCTGLPVQANKAIVGANAFAHESGIHQDGVLKARQTYEIMTPESVGLDSTRLVLGRH
                     SGRHGFKARLEKLGYRLDKDALERAYERFLAIADRKKEVFDEDLAAIVGDEFRDVPEV
                     YKLDYMHCISGSKILPSATVRIIVNGEVHQESEWGDGPVDAVFRAISKVSGFAITLDN
                     YTIRAVTAGTDAIGEVTLRVRDNGHKVTGRGASTDIVEASARAYVDGLNKLAAWHARV
                     AEQDGNQLGETV"
     gene            6100..7386
                     /locus_tag="P5179_04615"
     CDS             6100..7386
                     /locus_tag="P5179_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRS94536.1"
                     /translation="MGMTITEKIFAAHCGKKSVVPGELVNAKLDLVMCHDVTTPPAVE
                     MLEKRGMDRVWDPDKVVVTPDHFVPAKDILSAELHRSLDRWVKRHRIRHYYPLGRHGV
                     CHAILPEKGHILPGEVIVGGDSHTCTYGAFGAFSAGIGSTDLAAALYAGELWFRVPET
                     MKFTLNGVPPEGVYSKDIILEIIRRIGVDGARYMAMEYDGSTLAHLSMEARMTITNMA
                     IEAGAKSGIIPADHVTEAYIRERRHDRPNYRVYHSDPDAKYVAHHTIDVAAMEPMVAF
                     PNLPSNAHSIGDVRSDIGIDQAYIGSCTNGRIEDLRIAAAILKGHKVKARTMVVPATT
                     AIWKQANDEGLFDIFYDAECVISTPTCGACLGGHMGVLAADEVCISTTNRNFVGRMGS
                     PKAKVYLASPATVAASAIEGRIVDPRPYLTKTAAAE"
     gene            7426..7911
                     /locus_tag="P5179_04620"
     CDS             7426..7911
                     /locus_tag="P5179_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRS94537.1"
                     /translation="MRGRAHVYPRAHINTDEIIPARYLNVHSEAELAKHAMEDIDAEF
                     VKRVQPGDIIVAGDDFGCGSSREHAVWALRGAKVSAVIANNFARIYFRNSINNGFPAI
                     ECQDIAQKVQTGDELEIDLKAGSITNVTRKETYTFVPMADFAVEIMQAGGLLEYVKAK
                     A"
     gene            7942..8643
                     /locus_tag="P5179_04625"
     CDS             7942..8643
                     /locus_tag="P5179_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94538.1"
                     /translation="MLRRTTIILGTASILAAAAAGIRIERAWCARSALDTTRAPDDIA
                     ALPPLESTIAGLYEKPPMPGRRVLLTGPILVLDTIRTVRPIATSTDTIPVPEGLIFLA
                     DSLTGHRIVLLRRLDEDGSAWVELTPLPRSVSVPHENPRSTPLAHLAHFWQAGWSDLH
                     GPGGFVWLGLDSAIIRTRDIVNPDSPPYRLARVIRSGSFRVAKAPRVWDMANILLAVA
                     ALACALGAWRSRKKA"
     gene            8640..9986
                     /gene="hisD"
                     /locus_tag="P5179_04630"
     CDS             8640..9986
                     /gene="hisD"
                     /locus_tag="P5179_04630"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRS94539.1"
                     /translation="MRATERTAKEVPVRIPCIPYRTGDSPERLAAICARGDSASAAIR
                     ARVSTVLEAVRAQGDAAVVEFTEQFDHVRLTPAELRVPMTVCAEALESLEPPLAEALR
                     AAHDNIRRFHEHHRPASWTVDDGDGVVLGKRYAPVAAAGLYVPGGTAPLFSSVLMSVV
                     PAKVAGVPRIALCTPPSEDGMPSQATLAACALAGVDEVYRVGGIQAIAAMAFGTGTIA
                     PVDVIAGPGNAFVQEAKRQLAGTVGIDMVAGPSEIVVIADETANPAWIAADLLSQAEH
                     GSGSEASVAIVTSEDLGERIAREVDSRMRLLPRSAMVEKALSAYGTIFCVPDLGTACE
                     LANRIAPEHVELHTADPWALVDCIRCAGAIFIGAHSPEPVGDYYAGTNHILPTGRAAR
                     FASSVGVDTFLRATSLVAYTGQRLAKTAAHIVTLAEAEGLSAHARAAAIRVEHTSQ"
     gene            10006..10665
                     /locus_tag="P5179_04635"
     CDS             10006..10665
                     /locus_tag="P5179_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRS94540.1"
                     /translation="MKTAFWMRSAAGIGGTFLFVWLFAGCAAKVADVREEPETTVYAP
                     GADTVVTTPARPDVYATRTVRTRANVRTQPSTSADVVRVLDPGASVGLIAVHNGWFNV
                     MVDSVSGSTGWMWGPLLALTREDRFGAALDMAKPNFGADTLFSAVFYEDNILKVSLDL
                     AWRDLTTAQKQEVVSQIGTAWRAAAQQMGFRPAPEIRFLSNMNYEMARYTSKGQVVVK
                     H"
     gene            10713..11666
                     /locus_tag="P5179_04640"
     CDS             10713..11666
                     /locus_tag="P5179_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS94541.1"
                     /translation="MIFIPYDGAYLGKTGDLASFNFAVLGDSHTHRTANPEDPAPELL
                     RIIEELNLLRPAFAVSAGDLIRGYVNETEKAEIEATGDSPTLEKRLRLEHSGAVRAYE
                     SLAMPFYPCIGNHDVREPVSERVWRDIWGGRYYSFDYADCHFVVLDAEISKDTESVAG
                     EQLEWLERDLATYAVGKRLFISLHRPWWFDYPLHTATWKHPGGRNDWNDIVDPILRRY
                     DLQAIFCGHKHRFEFEHREGVPHIVTGGAGGEINDAPEIGGVVHYTWVSVYPGGFTWS
                     VIVPGHILSPERIARAKNNFREVIPWLPPEMGGTRKVRVED"
BASE COUNT         2397 a         3547 c         3722 g         2084 t
ORIGIN      
        1 aagaattgct cgtaccatgg cgcagacatg gcgtggtcct ttcgcgatta accgtttgtt
       61 gtttcgtctc aacggtacca cggtcgtcgg cgcgggggaa ggcgggcggg gccggtgcgg
      121 caagacattc ctgtctgcct tgatggagga cttctctatg ggcgccatga gatccttcgc
      181 ttccagcaca agcgatacgg ccgccgctca gaatgacgct cgcttttgaa cctcccgaag
      241 gcgctcttga catcgcacgg gcgcttttcc tattttttga actgactcaa gccctacacg
      301 cacgacttca gggttacgaa catgcgcacc agtctgattc catctctggc cgctgctgcg
      361 gcatcgcata tacggcgcac gtatgccgtg tcggcgcggg atcagattgt caactccctc
      421 gtcaccattc tcctccttgt actcgtactg gggctactcg taccggtaag ccggtagcga
      481 ggccccgttc gccgtcagtt ctcccctgga acacagacga atggagcctc ggatcggcga
      541 tccgcgggga gggatgttgt gtttttggcg ctcgcatgat ggaaaaaggg tataagtcca
      601 gtctaacagg caggatacgc acgatgaacc agtcaggagc acacgccggt cgcacgccgc
      661 gcacaatcac gcacgcgccg ttcgccggcg gaacggtacg ggaaaaccat tggcccggca
      721 cgacaatcag gtgaattcac ttcgtcaatt gacagcaaag gagccgagaa caatggctga
      781 ttccggtact gcgaaagccg ccaacagagc cttctggcgg cgcgatatgg aaggcaaaga
      841 gtgcaccggc gcggagatac tggtcgaatg catcatccgc gagggcgtgg acctcatgtt
      901 cggctacccc ggcggtgtgg tgctgaaggt atttgatgag ctgtactcac ggcgcgatca
      961 gattcgcgtg gttctgccgc gccacgaaca ggcaggggtg catgccgccg acggctgggc
     1021 gaaggtgacc ggcaaggtcg ggacggtgtt cgtcacgagt gggcccggcg ccacgaacgc
     1081 ggtgaccgga atcgccaccg cgcagatgga ttccgttccg ctggtggtgt tcacgggtca
     1141 ggtctcccgg gagttgatcg gttcggacgc gtttcaggaa acggatgcca tcggtgttac
     1201 tcggccgatc gtgaagcaca gctacatgat cgaacgcccg caggatatcg cgcgcatcgt
     1261 tcggcaggcg tactacatcg cctccaccgg ccgccccggc gtggtgctgg tggacgtgcc
     1321 gaagaacgtg acgttcgaaa aggcagtgtt cgaatggcct gagacggtga gcattcgaag
     1381 ctacaagccg accaccgagg ggcatcccaa atccatacag cggctggcgg acgcaatcgc
     1441 cgaatcaaag cggccgtacc tctatgtcgg cggcggcgtc gtcatggcga atgcctccga
     1501 tcacctgctc gaactggtga acaagaccaa tattcccgtt accaccacgt tgatggccct
     1561 cggcgcattc cccggcacgc atccgctgtt cgccggcatg cccggaatgc atggcagcaa
     1621 gctggcaaac atctgcttcc aggaatgcga ccttgtcatt gcggtgggcg cgcggttcga
     1681 cgaccgcgtg accgggaagc tttcggcgtt cgcgcccaag gcgaaaatcg cccacgtgga
     1741 cattgaccct tcttccatca aaaagacggt gaaggtggat taccccgtgg tgggcgacgc
     1801 acactgggtg ctgcgcgagc tgaacaaggt ggtgaaaccc cgccagccca acgagtggaa
     1861 ccgtacgttc gcggaaatga aggaaacgca ctggttccgt taccggccga gcaccgaagt
     1921 gatgcggcct cagtacgtga tcgagaagat ctacgagatg acaaaaggcg acgcgatcat
     1981 ctccaccgag gtggggcaga tccagatgtg ggcggctcaa ttctacaagt tcgacaagcc
     2041 gcgccggtgg ctgacctcag gcgggctcgg caccatggga tacggcttcc ccgcagcaat
     2101 cggggctcaa tttgccgcgc cggacgccat ggtgatcgac atcgcgggtg acgggtccat
     2161 tcagatgaac atccaggagc tcaccacggt ggtagaaaac cggctgccgg tgaaagtcgt
     2221 gatcctgaac aacggatacc tcgggatggt gcgtcagtgg caggagttct tctacgaccg
     2281 gcactactcg agcagcgtga tcggcgggtt gcctgatttc gcgaaagtag cggatgcgtt
     2341 cggcgcgaaa ggcctgaccg cgcgcactca tgacgacgtc gtgccggtgc tcgaagaagg
     2401 gctggcgcat ccgggcccgg tgttcatgga tttccacatc aacccggaag agaacgtgtt
     2461 cccgatggtt cccgcgggca aggctctgga cgagatgcag gaagaggagt gggcataaat
     2521 gaaccgccga cacatcatca gtgtgcttgt ggagaaccat ttcggcgtcc tggcgcgcat
     2581 cagcagtctg atcgccggac gcgggttcaa cattgacagc ctcaccgtcg gggaaacgga
     2641 agaccccacc gtctcccgca tgaccatcgt gacgcgtggc gacgagcaga tcatagagca
     2701 gatcaccaag cagttgaacc gtctgattga cgtcatcaaa gtcaccgaca tgaccgaatg
     2761 cgcgcatgtc gagcgcgaac tggtgctgat caaagtggcg gcgccgaccg cgcagacgcg
     2821 caccgaagtc gtgcaggtgg tggatgtgtt ccgcgcccgc gtggtggata tgagcacggc
     2881 cacgctcacc atcgaaatca ccggtgattc ggaaaaagtg gatgcgttca tcgagatgat
     2941 tgaaccgttt ggcatcaagg aaatggcgcg caccggttct gtcgccatgg ggcgtgaacg
     3001 gaacggcaga gccagccgtg gagcagaaac caaagttgcg taaattcaca gggggccacc
     3061 ccggcacatc ggggcagccc tctgtttttc gccggtcctg gtgattgtgc aacctgagtg
     3121 ttttcccgga aggattcgtg acagatgagc acgcagaaag aagaaaccac cgcccttgcc
     3181 accgaaaccc gcgcccgtat gtactacgac gacgacgcga atctggatct cctcaaagac
     3241 aaaaccgttg ccgtcatcgg gtgcggttcg cagggccacg cccacgcgct gaacctgcag
     3301 gactctggcg tgaaggtggt ggtaggcctg cggcaggggt cgaaaacgtg ggcgaaggcg
     3361 gaagcagcgg gcctgaaagt gctggaaacc gcagaagcgg cgaaacaggg tgacatcatc
     3421 atgatgctgg ttcccgacac cgccgcatcg gagatctacg agcgctacat cgcagcgaac
     3481 ctgacccccg gcaaggcgct ggcgtttgcg cacggcttca acatccattt caaaaccatc
     3541 gttccgccga caaacgtgga cgtattcatg gtggcgccga aaggtccagg ccacctcgtt
     3601 cgcgcgacgt acacccgtgg cagcggcgtt ccgaacctga tcgccatcta ccagaatccg
     3661 tccggcaagg cgaaggacat tgcgctggcg tatgccaagg gtatcggcgg aacccgcgtc
     3721 ggggtgatcg aaaccacgtt caaggaagag acggaaaccg atcttttcgg cgagcaggtg
     3781 gtgctgtgcg gtggtctgtc gaagctgatc cagacggcgt ttgagacgct ggtcgaggcg
     3841 gggtatcagc cggaaatcgc ctatttcgag tgcctgcacg aggtgaagct gattaccgac
     3901 ctcatctacg tcggcggcat ctccaccatg cgctactcta tctccgacac cgccgagtgg
     3961 ggcgattacg tgacgggcaa gcgcatcatc acggaagaga ccaggaacga aatgcgcagg
     4021 gtgctggcgg aaatccagag cggcaaattc gcgaaggaat gggttgccga gtacaaggcc
     4081 ggcctgccga atttcaagcg catgcgggaa gaacagtcgc agcatctgat tgagcagacc
     4141 ggcgcaccgt tgcgcgaaat gatgaagggc gcgctgaaac tcgaaggcgg cggcacggaa
     4201 aagttcctgt cggacaagta ggacggaacg gttcccatcg gtcgcatacg acgtatagga
     4261 cccaaagaac cgcaacccgg cagcttacat ccttccaccg gcacccacaa accggcccgg
     4321 tggtgatggc gaaaggtggt gatgggaaat ggacgacggc ctcgccgatc tcagcgacct
     4381 tctgtccctt atgcggggaa gagcgcactc gtcggcagcg tctctttccc gagccacgaa
     4441 taaccgcatg ccgacgggaa acgagacagc accatgagtg acgactcatc agtagtccgc
     4501 atattcgaca ccacgctgcg cgatggcgag caatctcccg gcgcatcgct gagtgtccag
     4561 gaaaagatcg atatcgcgca ccagctcaag cggctgggtg tagacatcat cgaagcgggg
     4621 ttccccgtca gcagccctgc gcagttcgaa gcggtggaac ggatctccga agaggtcgac
     4681 ggccccacca tcgccgcgtt ggcacgtgcg gtgccgctgg atattgaaac ggcagcccgg
     4741 gcggtgaagc cggcgaagaa gaaacggatt cacaccttca tcgccaccag tccgattcat
     4801 atggagcgca agctcaagaa gacacccgcc gaggtgctca cacttaccgg cgaggcggta
     4861 cggctggcga aatcgttctg cgacgacgtg gagttctcac cggaagatgc tggccgcagc
     4921 gaatggccgt ttctggtgga ggtgctgacc gcagccatcg aagccggcgc gacgacgctc
     4981 aatattcccg acaccgtcgg ctacatgatg ccggaagagt acgggcggat gatcgcccat
     5041 ctcatcgcgc acgtcccggg cagcgagaac gtgatctaca gcactcactg ccacaacgac
     5101 ctcggccttg cggttgccaa cagcatcgcc ggcgtggtga acggcgcgcg acaggtcgaa
     5161 tgcaccatca acggcctcgg cgaacgcgcc ggcaacgccg caatggaaga gtttgtcatg
     5221 gcgctgcgca cccggcagga cgtgttcaag ctccgtacga acatcgttac taccgaaatc
     5281 acccggacga gccgcatggt ggcccggtgc acgggccttc cggtgcaggc caacaaggcg
     5341 attgtgggcg cgaacgcctt cgcacacgag tccggcattc atcaggatgg cgtcctcaag
     5401 gcgcggcaga cgtacgaaat catgacgccg gagtccgttg gcctcgattc aacccgactg
     5461 gtgctggggc gacattcagg acggcacggt ttcaaagcgc ggctggagaa actcgggtac
     5521 cggctggaca aagacgctct ggaacgcgcg tacgagcggt tcctcgccat cgctgaccga
     5581 aaaaaggaag tgttcgacga ggacctggcc gccatcgtgg gcgatgagtt ccgcgacgtt
     5641 cccgaggtgt acaaactcga ttacatgcac tgcatcagcg gaagcaaaat cctgccctcg
     5701 gcgaccgtgc ggattatcgt gaacggcgag gtgcatcagg aatcggaatg gggcgatggg
     5761 ccggtggacg ccgtatttcg cgcgatctcc aaggtatccg gttttgcaat cacgctggat
     5821 aactacacca tccgcgcggt aacggcggga acggacgcca tcggcgaggt aaccctgcgc
     5881 gtgcgggaca acggccataa ggtcaccggg cgaggtgcca gcaccgacat cgtggaagcc
     5941 tccgcgcggg cgtatgtgga tgggctcaac aagcttgccg catggcatgc ccgagtggcc
     6001 gaacaggatg gtaaccagct cggagaaacg gtctgatttc actacgccgc cggctatctc
     6061 cgcagagcag cggggggaat gaagaagaag ggtaacacca tgggaatgac gattacggaa
     6121 aagatatttg ccgcacactg tggcaagaaa agcgtggttc cgggcgaatt ggtcaacgcg
     6181 aaactcgatc ttgtcatgtg ccacgacgtc accaccccgc ctgcggtgga gatgcttgag
     6241 aaacgcggca tggacagggt gtgggacccc gacaaagtcg ttgtgacgcc ggaccatttc
     6301 gtgccggcaa aggacatcct gagcgcggag ttgcacagga gcctcgaccg gtgggtgaaa
     6361 cggcaccgga tcaggcacta ctatcccctc ggccgccacg gcgtctgcca tgccattctg
     6421 cccgagaagg ggcacatcct tccgggcgaa gtcatcgtcg ggggggattc gcacacctgc
     6481 acgtacggcg cgttcggtgc gttttccgcc ggaatcggca gcacggacct cgccgccgcg
     6541 ctgtatgcgg gtgagctctg gttccgcgtg cccgaaacaa tgaagttcac gctgaacgga
     6601 gtgcctccgg aaggcgtgta ttccaaggac atcatcctcg agatcatccg ccgcataggg
     6661 gtggatggtg cccgctacat ggcgatggag tacgacggaa gcacgcttgc ccacctgagc
     6721 atggaagccc gcatgaccat caccaacatg gcgatcgagg cgggggcgaa atcgggtatc
     6781 attccggcgg accatgtcac ggaggcgtat atccgtgagc gccggcacga caggccgaat
     6841 taccgggtgt atcactccga ccccgacgcg aagtacgtcg cccaccacac gattgacgtg
     6901 gcggccatgg aaccgatggt ggcgtttccg aacctgccgt cgaacgctca ctccattggc
     6961 gacgttcgct cggatatcgg gatagaccag gcgtacatcg gctcctgcac caacgggcgc
     7021 atcgaggatc tgcgcatcgc cgcggcaatt ctcaaagggc acaaggtgaa ggcccggacc
     7081 atggtggttc cggcaactac cgccatctgg aaacaggcaa acgacgaagg ccttttcgac
     7141 atcttctacg atgccgaatg cgtcatatcc acgcccacgt gcggggcctg tctcgggggg
     7201 catatgggcg ttctggcagc cgacgaggtg tgcatttcca ccacgaaccg caacttcgtt
     7261 ggacgcatgg gaagcccgaa agccaaggtg tatctcgcaa gccctgccac ggtggcggca
     7321 tcggcaattg agggaagaat cgtggatccg cggccctacc tcacaaaaac ggcggcggca
     7381 gaataggcac tgacgattgc atcgacagat acaaggagta ttgccatgcg tggacgcgcc
     7441 catgtctatc cccgcgcgca catcaacacc gacgaaatca tccccgcgcg gtacctgaac
     7501 gtccactccg aagcagagct cgcgaagcat gcgatggagg acattgacgc cgagttcgtg
     7561 aaaagggtgc aacccggcga tattattgtc gcgggggacg atttcggctg cgggtcatcg
     7621 cgcgagcacg ccgtctgggc actgcgcggg gcgaaagtca gcgccgtgat cgcgaacaac
     7681 ttcgcgcgta tctacttccg caacagcatc aacaacgggt ttccggccat cgagtgccag
     7741 gacatcgcgc agaaggtgca gaccggtgac gaactggaga tcgatctcaa ggcggggagt
     7801 atcaccaacg ttaccagaaa agaaacctac accttcgtcc cgatggcgga tttcgcggtc
     7861 gagatcatgc aggcgggcgg tctgctggag tacgtaaagg cgaaagcctg aaagagctcg
     7921 cgctatcggg ggaggattgc cgtgcttcga cggaccacga taattctagg taccgcatcg
     7981 attcttgcgg cggcggcggc aggtatccgt atcgagcggg catggtgtgc gcggtcggcg
     8041 cttgatacca cgcgcgcgcc ggacgatatc gccgcgttgc ctcccctcga gtcgaccatc
     8101 gccggtttgt atgaaaagcc cccgatgccc ggccgccgcg tgctccttac aggcccgatt
     8161 ctggtgctcg acacgatcag aacggtgcgg ccgatcgcca catcgactga tacgatcccg
     8221 gttcccgagg gtctcatctt tctggcggat tctctcaccg gtcatcggat cgtcctgctc
     8281 cgccgccttg acgaggacgg gtccgcgtgg gtggagctta cgccgcttcc gcgatcagtg
     8341 tccgtgcccc atgaaaatcc ccggtccacc ccgcttgctc atctcgcgca tttctggcag
     8401 gcgggatggt cggacctgca cgggccgggc ggtttcgtct ggctgggtct tgattcggca
     8461 attatcagaa cgcgcgacat cgtgaatccg gattctccgc cctaccgcct ggctcgcgtc
     8521 atccgttccg ggtcgtttcg cgtggcgaaa gcgccgagag tgtgggatat ggccaacatc
     8581 ctgcttgcgg tggcggccct tgcgtgcgcg cttggtgcgt ggcgctcacg gaagaaggcg
     8641 tgagggcaac ggaaaggaca gcaaaagagg ttcccgtgcg tattccctgc attccgtacc
     8701 gaactggcga ttctcctgag cgcctcgcgg ccatctgcgc ccgtggagat tccgcgtctg
     8761 cggcgatccg ggcgcgtgtc tccaccgtgc tggaggcagt gcgcgcacag ggcgatgcgg
     8821 cagtagtgga gttcacggag cagttcgacc acgtgcgcct gacgcctgcg gaattgcgcg
     8881 ttcccatgac ggtctgcgcg gaggcgctcg aatcactgga gccaccgctc gccgaggcgc
     8941 ttcgcgccgc gcacgacaac atccgccgct ttcatgagca ccaccgcccg gcgtcgtgga
     9001 cggtagacga cggcgacggc gtggttctgg gcaaacgata tgcgccggtt gccgcggcgg
     9061 ggctgtacgt tccgggtgga actgcgccgc tcttttccag tgttcttatg agtgtggtgc
     9121 ccgcgaaagt cgccggagtt ccccgaattg ccctgtgtac cccgccatcc gaagacggaa
     9181 tgccttcgca ggccacgctt gccgcgtgcg cgctcgccgg ggtggacgag gtctaccgcg
     9241 ttggaggaat tcaggcgatc gccgcaatgg cgttcggaac cggcacgatc gccccggtgg
     9301 acgtcatcgc cgggccgggc aacgcattcg tgcaggaagc gaagcgccag cttgcgggca
     9361 ctgtcggaat cgacatggtg gccgggccga gtgagatcgt cgtaattgcc gacgaaaccg
     9421 cgaacccggc gtggattgcc gccgatctcc tctcccaggc cgagcacggc agcgggtccg
     9481 aggcgtcggt tgcgatcgtc acgtcggagg atctcggcga gcgaattgcc cgcgaggtgg
     9541 actcacgcat gcgtctgctt ccccgttcgg ccatggtgga aaaggcactg agcgcatacg
     9601 gaaccatctt ctgcgttccc gaccttggaa cagcgtgcga actggcaaac cgcatcgctc
     9661 cggagcacgt ggaactccac acagccgatc catgggcgct tgtggactgc atacggtgcg
     9721 cgggagccat cttcatcggt gcacactcgc cggaaccggt gggcgactac tacgccggca
     9781 cgaaccatat tctcccgacc ggcagagcgg ctcgtttcgc ttccagcgtc ggggttgata
     9841 cgtttcttcg cgcgaccagt ctggttgcgt ataccgggca gcggctcgcg aaaaccgccg
     9901 cccatattgt cacgctggcc gaagcggaag ggctttctgc ccacgcccgc gcggccgcga
     9961 ttcgggtcga acacacttcg caatgaggac acacgaaggg acgccatgaa aactgctttc
    10021 tggatgcgct ctgctgcagg tatcggcgga acgtttcttt ttgtctggct gtttgccgga
    10081 tgcgcggcca aggtggctga cgtgcgggaa gagccggaaa ctacggtcta cgcgccgggt
    10141 gccgataccg ttgtcaccac tcccgcccgc cccgatgtgt acgccacgcg caccgtgcgc
    10201 acgcgggcca acgtccgcac ccagccctcc accagcgccg acgtggtcag agttctggat
    10261 ccgggggctt cagtggggct gatcgcggtt cacaacggat ggttcaacgt catggtggat
    10321 tccgtttccg gctccaccgg gtggatgtgg ggaccgttgc tcgccctcac ccgggaggac
    10381 cgcttcggcg cggcgctcga tatggcgaag ccgaatttcg gggccgatac cctgttctcc
    10441 gccgtgttct acgaggacaa catcctcaag gtaagcctcg acctcgcgtg gcgggacctc
    10501 accaccgcgc agaaacagga ggtcgtgagc cagataggca ccgcatggcg cgccgccgcc
    10561 cagcagatgg gcttccgtcc ggcgccggaa atccgcttcc tttccaacat gaactacgaa
    10621 atggcgcgtt acaccagcaa gggacaggtc gtcgtcaagc actgatcgtg tccgacgttg
    10681 ctccgctgtc tgctcgcaaa gaaggggcgc ctgtgatttt cattccgtac gatggagcat
    10741 atctcggcaa aaccggcgat ctggcttcgt tcaacttcgc cgttctgggt gattcacaca
    10801 cacaccgcac tgccaatccc gaggatccgg cgccagagct tctccgcatc atcgaggagc
    10861 tgaacctcct ccgccccgcg tttgccgtct cggcgggcga cctcatccgc ggctacgtga
    10921 acgagacgga aaaggcggaa atcgaggcga ccggggattc ccctaccctg gaaaagcggc
    10981 tgcggctgga gcactccggc gccgtacggg cgtatgaatc actggccatg ccgttctatc
    11041 catgcatcgg caaccacgac gtccgcgaac cggtcagcga gcgcgtctgg cgggatatct
    11101 ggggcgggcg ctactactcc ttcgactacg ccgactgcca ctttgtggtg ctggacgccg
    11161 agatttcaaa agacaccgag agtgtcgccg gcgagcagct cgagtggctc gaacgcgacc
    11221 ttgccacgta cgccgtcggc aagcgcctct tcatcagcct ccaccggccc tggtggtttg
    11281 attacccgct ccataccgcc acgtggaaac accccggcgg ccggaacgac tggaacgaca
    11341 tcgtcgaccc gatcctccgc cggtacgacc ttcaggccat cttctgcggc cacaagcacc
    11401 ggtttgagtt cgagcaccgg gagggcgtgc cgcacatcgt tacgggcggc gcggggggcg
    11461 agatcaacga cgcaccggaa atcggcggcg tggtgcacta cacctgggtg agcgtctacc
    11521 ccggcgggtt cacatggtcg gtgattgtgc cgggccacat cctcagccct gaacgcattg
    11581 cccgggcaaa gaacaacttc cgggaggtca tcccgtggct gccgccggaa atgggtggga
    11641 cccgcaaagt ccgcgtggag gattgagcgc gcccgccgcg ccacggacgg gtagggcggt
    11701 ctgcctgcac caacgcgtgg tggctgagct tgtcgaagcc ccgcgcggcg
//
LOCUS       DAOTNG010000064        11686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5228_length_11686_cov_5.113576, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000064 DAOTNG010000000
VERSION     DAOTNG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_11686_cov_5.113576"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="P5179_04645"
     CDS             <1..1073
                     /locus_tag="P5179_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94542.1"
                     /translation="GLSPDQEVSLFVEGDARRVSGPEATANEKTLASVISWQNTTGRF
                     WIKGNVQADFTSTARPAGTDGTGSFYAVSAEAWTRPEENFGGAVGVTIYTVNYLSGDS
                     LRTIRPSIAAWGRVRNLVKFTGRLSSGVQRFGIWEAYQANQMLNLATPLRSPFTSVDI
                     DLNVETSVHRDNVLTFGFRQLIVDDYAVWVRKDSTVTWRRRFPTEPHTVNQFGVDYGY
                     AGTDQASLTSFYARYQYAWREGSVDALGLIRAHSLVQQPVPYVPDWEFHLGARFPFRR
                     FTVAPSLHTIGTRHYIEPKTLDQIAEMDPYVLVDLEVSTALNRHWQLALLGSNLLNSR
                     YQRWNGFNEPGIYGQIGIRRTW"
     gene            1140..1745
                     /locus_tag="P5179_04650"
     CDS             1140..1745
                     /locus_tag="P5179_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS94543.1"
                     /translation="MTLLSLAIQGGWLMIPIALCSIIGVWVYVERYLTYRRVRLDGIS
                     LLEQIRPAVTSRQTAEVERRLAMNPAPVGQVIAAGLRSLAGGRDAVREAMENAGNRQI
                     HLLEKRLSILATVAGVAPMLGFLGTVTGMIIAFQQIENLGGNVNATVLAGGIWQAMIT
                     TAFGLIVGIPALIGYNHLSGIVQEFAADIEHAGAEFLTLVN"
     gene            1762..2160
                     /locus_tag="P5179_04655"
     CDS             1762..2160
                     /locus_tag="P5179_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS94544.1"
                     /translation="MKLRKPQGAITAFAAQSLTDIVFLLLIFFLLTSTFVLQTGVKVQ
                     LPQTTADEPVSEKMVVISLTKDGSVYLNENLVSRPELVGRLRQLLVSKEQIVILRADK
                     SLSLDLVVGVMDIAKSSGASRFLIATQTEE"
     gene            2166..2939
                     /locus_tag="P5179_04660"
     CDS             2166..2939
                     /locus_tag="P5179_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94545.1"
                     /translation="MRASWTETPQARNRTAAIVSFGLHGLLAAAAAFFAITVATPPPE
                     FVELNVGRLSQAELNRLIQEGERASAAASPEERAQTPQQRLPDIEMPTISPTEAERRL
                     MPTQVALKENKVFVAPPRPTAIAAPPIPVQSIDRKVRYEGGQISLGPRPGEGIVSEHV
                     GSDIHPVFQIEGDLIGRRFYEAAITETPNVPAQTRIQFDLVVSPSGAVISALVVRREN
                     SDLEAFAANYLRRCRFDPLPAGMPQENQTGRITITFSAG"
     gene            2961..3482
                     /locus_tag="P5179_04665"
     CDS             2961..3482
                     /locus_tag="P5179_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRS94546.1"
                     /translation="MAHAVWANPKSLFRPRTPLAKALGDLPLFSRLSRAEIEKVEAIA
                     HIRTYSAGECIFEQGDPSVAMFVIMAGEVRIVIRTEEAEEDREVARLGTGEMFGETAL
                     LDGAPRTAAAIAATPAEIAAIARPDWIDLVERHPALGVRMLLPLARVMAARVRALNLQ
                     VPTSQQQNWEQYV"
     gene            3479..4567
                     /locus_tag="P5179_04670"
     CDS             3479..4567
                     /locus_tag="P5179_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRS94547.1"
                     /translation="MNTGSSAAKFGWIRLTALGVGCAIGLYVIRDVLPPIVLAAIMAY
                     ILNPIVDMGERRSVPRWVTIILIYLLITAAAVVGVLFLLPLLGQQFEEIRSQIGPFWS
                     QMQVYGYRFLDRIERWWPPLARVILAEVTSDALTTMPHQWLASALENAPAFISGAFSN
                     VVGIITYFITVPFIAFFLVRDGRAFKRAFIELVPNRYFETVVSVLDRIDHSVGLYIRG
                     ILLEALLLGTLATIGLLVIGLKGAILIGMLTGFLNLIPYVGAIVGFVTGVFVALATGG
                     NVPGVVIVFAFSQFVDNWFIQPFVLSRSVNLHPLLIFLAVVFGGVYGGLLGMFLAVPL
                     TGAFVVTARTIREGLRPPVYPYDVIAEL"
     gene            complement(4585..5361)
                     /locus_tag="P5179_04675"
     CDS             complement(4585..5361)
                     /locus_tag="P5179_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS94548.1"
                     /translation="MITAVIIDDEQPARDELRYLLRKHADIEVIGETASGTAAVELIG
                     ERAPDLVFLDIQMPGMTGFQVVQELMDREEELPAIVFVTAYDEYAVRAFEVSAVDYLL
                     KPVEEERLAQALEKVRRQAAATALSRGEISRLVRQMEEGERTATLPHRISVRKGDRYV
                     LVDPNDVTHAYVVDGVVCLCTSKLNGMTTHKTLEELEKDLDGRIFWRVHRSYIVNINH
                     VAEILPLPTGSYRLRLDDEKKTTVPLSRAQAKLLRKVVKW"
     gene            5549..6118
                     /locus_tag="P5179_04680"
     CDS             5549..6118
                     /locus_tag="P5179_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRS94549.1"
                     /translation="MRAVVLNGLREGDVGQAISDEVRSRLEHAGWTTEEFVLRDRNVA
                     PCVEDFGCWIRKPGQCLIKDDAQEITRAFLTCDLVVFLTPVTFGGYSSELKKVLDRSI
                     CFALPYFEGVGGETHHPTRYDRIPAMFYVGTETTPDEQAESVFRELVRRNALNMRPRA
                     YRVSFVRAEKDAVRVVEDVLALANEEYGS"
     gene            6115..6804
                     /locus_tag="P5179_04685"
     CDS             6115..6804
                     /locus_tag="P5179_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94550.1"
                     /translation="MTGKPRILFAVGSQKPAESNSRSLADYLAEQLRKRGVPSKQIHL
                     LPLVRGEERWNELFAAVDEADFLFLVFPLFVDSQPWSVVRFMELVGAHRRSSNAGAMR
                     LAALVNSGFPEARHNEPALAICRQFAVESNMSWAGGLALGSGPVIGGKPLPDAGRTVR
                     PVMKALEMVADALAEGRDIPAEAIALMARPLMPHWVYRSVATLGLWWTARKNGVSGKL
                     RARPFTSPAEP"
     gene            6966..7529
                     /gene="pduL"
                     /locus_tag="P5179_04690"
     CDS             6966..7529
                     /gene="pduL"
                     /locus_tag="P5179_04690"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HRS94551.1"
                     /translation="MVPVGISARHCHVTEEVLQILYGRGAKLHPYRELRQPGFYAARE
                     TVTVLGPRMRAIENVRILGPTRTYTQVELSRTDGFLLGLSLPIRDSGDLEGTPGVTLV
                     GPAGSITLPNGAMRAGRHVHMSPEVAAAWGLTDRQIVRAEFEGEKGVVFSNVLIRVGK
                     GQVLEMHLDTDDGNAADLVTGDMVRLF"
     gene            7731..8843
                     /gene="gmd"
                     /locus_tag="P5179_04695"
     CDS             7731..8843
                     /gene="gmd"
                     /locus_tag="P5179_04695"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS94552.1"
                     /translation="MRKKALITGITGQDGAYLSRLLLEKGYEVHGIKRRSSQFNTARI
                     DDLYQDPHESDRRLFLHYGDVTDALHMFQIVKNVQPDEIYNLAAQSHVAVSFQTPEYT
                     ANVDAIGPLRILEAIRNADLVGKTRFYQASTSEMFGMVQETPQTEKTPFYPRSPYAAA
                     KLYAYWVTVNYREAYGVFACNGILFNHESPMRGETFVTRKITRAVARIKHGLQKCLYL
                     GNLDALRDWGHARDFVEAMWLMLQQERPDDFVIATGEQHSVREFVVLAFREVGIEIEW
                     KGSGDNEHAIATGPVCEGRSPDDNIVVAVDPRYRRPSEVETLLGDATKARTQLGWVPR
                     TTFNELVKEMVAGDVELVERDLLVRRHGYSTPDYHE"
     gene            8916..9896
                     /locus_tag="P5179_04700"
     CDS             8916..9896
                     /locus_tag="P5179_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRS94553.1"
                     /translation="MPTDAKIYVPGHRGLVGSAIVRRLESLGYRNLILRTRAELNLLD
                     ARAVASLFAEEKPEFVFVCAAKVGGIMANNTYPAEFFYENVAIETAIIHEAWRNNVRR
                     LLFLGSSCIYPRDCPQPIREEYLMTGPLEPTNRAYAVAKIAGIEMCRAYNQQYGTRYL
                     AAMPTNLYGQGDNYDIQTSHVLPALIRKMHEAKIRGDASVTLWGTGRPYREFVYSDDL
                     ADACVFLLNLDDERFGMLLGDGSSNPLINVGCGEDLTIADLAGLVKDVVGFEGTIVWD
                     TTKPDGTPRKLLDVSKLNGLGWRPSIPLRDGITRAYRQFLEHENNARGRS"
     gene            9941..10570
                     /locus_tag="P5179_04705"
     CDS             9941..10570
                     /locus_tag="P5179_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HRS94554.1"
                     /translation="MDWYGIIKTTVGILAIVNPLGAVGTFLSATENLPAAARRKTADT
                     AAVTVTVTLLVAAWVGETILHFFGISIPAFRVGGGILVLSMAISMLHARPSPAKQVPE
                     EAADAVERQAVGVVPLGIPLMSGPGAIALVIVESHRAPTVPERLLVSLCIVLVAVVSW
                     TILRLAGLLAKRLTRTHINISTRLMGLILAAIAVEFIASGALVLFPGLR"
     gene            complement(10576..11310)
                     /locus_tag="P5179_04710"
     CDS             complement(10576..11310)
                     /locus_tag="P5179_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528096.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRS94555.1"
                     /translation="MSEKFPQQFAGMLALALALVIASFVGYAAVNRAKRAGDTITVTG
                     SAKKAITSDRVVWRGSVSAQKPSMQEAYGDVKRYSERVRAYFREQGIPDSAVTFQAMQ
                     TNQIQEVLSGGHMTGRILAYQIFQGFEIQSEDVDRITRLSADAPDLMKEGVPISSNPP
                     EFVYSNLASVRVEMLAEAAQDARKRAETIAQAVGSRIGAVRSARMGVFQITPRNSTMV
                     SDYGINDTSSREKDVTAVVSVTFAVE"
     gene            complement(11462..>11686)
                     /locus_tag="P5179_04715"
     CDS             complement(11462..>11686)
                     /locus_tag="P5179_04715"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HRS94556.1"
                     /translation="ALKKAEDNDDWVLHLYETTGAATDVTITFDRPVVEAYDTDLMEW
                     NRGVRLNMEGGTVRRSFRPWEIAGIRIRLG"
BASE COUNT         2210 a         3519 c         3536 g         2421 t
ORIGIN      
        1 gcggcctctc ccccgatcag gaggtaagcc tcttcgtaga gggcgatgcg cgccgcgtca
       61 gcggtcctga agcgacagcg aatgaaaaaa cgttggcctc cgtgatttcg tggcagaaca
      121 ccaccggccg cttctggata aaagggaacg ttcaggcgga cttcacaagc accgcgcgcc
      181 cggcaggcac ggacggcacc ggttcattct acgcggtaag tgcggaggcg tggactcgcc
      241 ccgaagagaa cttcggcggt gcggtcggcg tcaccatcta caccgtcaac tacctgtccg
      301 gcgattccct gcggacgatc cgcccgtcga ttgctgcgtg gggccgggta cgaaatctcg
      361 taaaattcac cgggcgtctt tcctccggcg tgcaacggtt tggaatctgg gaagcctatc
      421 aggccaatca gatgctgaac ctcgccacgc cccttcggtc gcccttcaca agcgtggata
      481 tcgatctcaa tgtagaaaca tccgtccacc gggataacgt tcttacgttc gggttcaggc
      541 agttgattgt cgacgattac gcggtctggg tccggaagga cagtaccgtc acgtggcggc
      601 ggcggttccc gacggaaccc cacaccgtga accagttcgg tgtggactac ggctacgcag
      661 gcaccgatca ggcgtcgctc acgtcgttct atgcgcggta tcagtacgcc tggcgcgaag
      721 gatccgtgga tgcgctcgga ctgattcggg cgcactctct cgtgcagcag cctgttccgt
      781 atgttccgga ctgggagttt caccttgggg cgcgtttccc gttccgacgc ttcaccgtcg
      841 cgccctcgct gcacaccatt ggaacccgac attacattga accgaaaacg ttggaccaga
      901 ttgcggaaat ggacccctac gtccttgttg atctcgaagt atctaccgcg ctgaaccgcc
      961 actggcagct ggccctgctc ggatcgaacc ttctcaacag cagataccag cgctggaatg
     1021 ggttcaatga accgggtatt tacggccaga ttggcattcg acgtacatgg tagaatgtgc
     1081 ggagtggatt ttgtaacgag ttccttaacg gagccggttt gtaaccaagg agtacggaga
     1141 tgacgttgtt gtcgctggcg attcagggcg gatggttgat gatcccgatt gcgctctgtt
     1201 caattatcgg tgtctgggtg tacgtggaac gctacctgac gtaccggcgg gttcgactgg
     1261 acggcatctc gctcctggag caaattcgac cggcggtcac ttcgcggcag accgcggagg
     1321 tggaacgccg gctcgcgatg aatcccgcgc ctgtcggtca ggtgattgcg gccggactgc
     1381 ggagcctggc aggtgggcgc gacgccgtcc gggaggcgat ggagaacgcc ggcaacaggc
     1441 agatccacct tttggagaaa cgcctttcca tacttgccac ggtcgccgga gttgcaccca
     1501 tgctgggctt tctcggcacg gtaaccggta tgataatcgc gtttcaacag attgaaaatc
     1561 tcgggggaaa cgtgaacgcg acggttctgg cgggaggtat ctggcaggca atgattacca
     1621 ccgcattcgg ccttatcgtc ggcattcccg cgcttatcgg atacaaccac ctgagtggaa
     1681 tcgtacagga gtttgccgct gacatcgagc atgccggtgc cgagtttctt accctggtga
     1741 actgacggga gcgctgcgat tatgaaactt cgcaaaccac aaggcgcgat caccgcgttc
     1801 gccgcgcagt ctctgacgga tatcgtgttt ctcctgctca ttttcttcct gcttacctcc
     1861 acgttcgttc tccagaccgg cgtgaaggtg caattgcctc agacgaccgc ggacgaaccc
     1921 gtgtcggaga agatggtcgt gatttctctc acgaaggacg gctccgtgta cctgaacgag
     1981 aacctcgtca gccgcccgga attggtggga aggcttcggc agttgctggt gagcaaagaa
     2041 caaatcgtga tccttcgtgc ggacaaaagc ctctcactgg atcttgtggt gggagttatg
     2101 gatatcgcga aatcgtcggg agcgtcgcgc ttcctgatcg caacgcagac cgaggagtag
     2161 gtcggatgcg cgcatcgtgg acagaaacgc cgcaggcgag aaaccgtacc gcggcgatag
     2221 tgtccttcgg cttgcacggg ttgttggctg ctgcggccgc atttttcgcg attaccgtgg
     2281 caaccccgcc gcccgagttc gtcgagttga acgtggggcg gttgtctcag gcggagctca
     2341 acagactgat tcaggagggc gagcgagcgt ccgctgctgc ctcgccggag gaacgggcac
     2401 agacgccgca gcaacgtctc cctgatatcg aaatgcccac gatcagcccg acggaggcgg
     2461 agcgccgcct tatgccgacg caggtcgcgc tgaaagagaa caaggtgttc gtggcgccgc
     2521 cgcgcccgac cgcgatcgcc gcccctccaa tcccggtgca gagcatcgat cggaaagttc
     2581 gctacgaggg cggacagatc agtctggggc ccaggccggg tgagggaatt gtgtccgaac
     2641 acgtgggaag cgacattcac cctgtgttcc agattgaggg agacctgata ggcaggcggt
     2701 tctacgaggc cgcgatcacg gaaaccccca atgtgccggc gcagacccgt atccagttcg
     2761 acctggtcgt ttcgccgtcc ggggcagtaa tcagcgcgct cgtcgttcga cgcgaaaact
     2821 ccgatctgga agcattcgct gccaattacc tgcgccgctg ccggttcgat ccgttgcccg
     2881 cggggatgcc gcaggaaaac cagaccggcc gaatcaccat cacgttctct gcaggttgaa
     2941 tctgcctgca ggaggaatac atggcgcatg ccgtgtgggc aaatccaaaa tcgctcttcc
     3001 gaccgcgcac gccgctcgcg aaggccctgg gtgaccttcc cctgttctcc cgcctttccc
     3061 gcgcggaaat cgaaaaggtg gaggccatcg cacacatccg tacctacagc gcgggcgagt
     3121 gcattttcga acagggcgac ccgagcgtgg cgatgttcgt cataatggcg ggtgaggtgc
     3181 gtatcgtcat ccggaccgag gaagccgagg aagaccggga ggttgcgcgg ctcggcacgg
     3241 gagaaatgtt cggggaaacc gcgctgctcg acggtgcgcc gcggactgcg gccgccatcg
     3301 cggccacacc ggcggaaatt gctgcaattg cacggccgga ctggatcgac ctcgtcgaac
     3361 ggcatccggc ccttggtgtt cgcatgttgc ttccgcttgc gcgggttatg gcggcaaggg
     3421 ttcgcgcgct gaacctgcag gtgccgacct cgcaacaaca gaactgggag cagtacgtat
     3481 gaacaccggc agctcagcag cgaaattcgg gtggatccgg ctgacggctc tcggtgtggg
     3541 gtgcgcgatc gggttgtacg tgatccgcga tgtgttgccg ccgatcgttc tggcagccat
     3601 catggcgtac atcctcaacc cgatcgtgga tatgggcgag cgcaggtctg tgccccggtg
     3661 ggtcacaatc atcctgatct atctcctgat caccgctgcg gcagtggttg gcgtcctgtt
     3721 cctgttgccg ttgcttggcc agcagtttga ggagattcgc tctcagatcg gtcctttctg
     3781 gtcgcaaatg caggtatatg gctaccgttt cctcgaccgg atcgagaggt ggtggccccc
     3841 gctggcgcgc gtgattctgg ccgaagtcac cagcgatgca ctgacgacga tgccacacca
     3901 gtggctggca agcgcactgg aaaacgcccc cgcgttcatt tccggagcat tctcaaacgt
     3961 tgtcggcatc atcacttatt tcattacggt gcctttcatt gccttttttc ttgtgcggga
     4021 tggccgcgca ttcaaacggg cgttcatcga gctcgttccc aaccggtact tcgaaaccgt
     4081 cgtcagcgtg cttgacagaa ttgaccactc agtcgggctt tacatccgtg ggattcttct
     4141 cgaggcactt ctcctcggta cactggcgac catcggattg ctcgtcatag gcctcaaggg
     4201 agcgattctg atcggcatgc tgacggggtt ccttaacctc attccctacg tgggcgcgat
     4261 cgtgggtttt gtcacggggg tatttgtcgc gcttgcaact ggtgggaacg ttccgggcgt
     4321 ggtgattgtg ttcgcgttca gccagttcgt ggataactgg ttcattcagc cttttgtgtt
     4381 gtcacggtcg gtaaacctgc accctctcct catctttctc gcggtcgtgt ttggtggcgt
     4441 gtacggcggc ctcctcggga tgtttctcgc cgtccccctg accggcgcgt tcgtggttac
     4501 tgctcgcacg atccgggaag gccttcgtcc tccggtctac ccgtacgatg tgatcgctga
     4561 gctgtagccg ccgcttcgcg cttctcacca cttcaccact ttcctcaaca gcttcgcctg
     4621 cgccctgctg agaggaacag tggttttttt ctcgtcgtcg aggcggaggc ggtaggatcc
     4681 cgtcggaagt ggcaggatct ccgcgacatg attgatgttg acgatgtagc tccggtggac
     4741 gcgccagaag attctcccgt caaggtcctt ttccagctct tcgagcgttt tgtgcgtagt
     4801 catcccgttc aacttcgatg tgcacagaca tacaacaccg tccacgacat aagcgtgcgt
     4861 cacgtcgttc gggtccacca gtacgtaccg gtcgcctttt cgtacggaga tccggtgcgg
     4921 gagtgtggcg gttcgttcac cctcttccat ctgccggacc agccgggaga tttccccccg
     4981 tgagagcgca gtcgccgcgg cctgcctgcg cactttttcc agcgcctgcg cgagccgctc
     5041 ctcttccacc ggcttcagga ggtaatccac tgctgaaact tcaaacgcgc gcaccgcgta
     5101 ctcgtcgtat gcggttacaa acacgattgc cgggagttct tcttcccggt ccatcagttc
     5161 ctgcaccacc tgaaatcctg tcattcccgg catctgtatg tccagaaaga caagatcggg
     5221 cgctcgctcg ccgatcaatt ccaccgccgc cgttccgctg gcggtctccc cgatcacttc
     5281 gatatcggcg tgcttcctca ggagatagcg gagttcatca cgtgcgggct gttcgtcatc
     5341 tatgatcact gcagttatca ttgtccaacc ctttccctct tcgctgccgg agcgttgttc
     5401 agagtccggt ctgacacgtt gttccgtgcg tctaaaatat cgcgcgcaac cggcggcgct
     5461 gtgaaatcca tcctgatggc gcccgcatta cacggcgagt atctttacag gaggacatcg
     5521 tcttgcagga tgcgtgcacg ggggtagcat gagagctgtg gttctgaacg gtctgcgcga
     5581 aggggacgtc gggcaggcga tctcagatga ggtacgttcc cgcctcgagc acgcggggtg
     5641 gacaacggag gagttcgtcc ttcgcgaccg gaacgttgcc ccctgcgtgg aggacttcgg
     5701 ttgctggatt cggaaacccg gccagtgcct cattaaggat gacgctcagg agataacccg
     5761 cgcgttcctg acctgtgatc tggtggtctt ccttactccc gtcacgttcg ggggctactc
     5821 gtccgaactc aagaaagtgc ttgaccggag catctgtttc gcgctcccgt atttcgaggg
     5881 agtaggcggc gaaacgcacc atccgacacg gtacgaccgg attccggcca tgttttacgt
     5941 cggaacggag accaccccgg acgaacaggc ggagagtgtt ttccgggaac tggtccggcg
     6001 caacgcgctc aacatgcgtc cgcgggcgta ccgggtctct ttcgtgagag ccgagaaaga
     6061 tgccgtccgc gtggtggaag acgtgctggc tctcgcgaac gaggagtatg gatcgtgacg
     6121 gggaagccga ggattctctt tgcggtgggg agccagaagc ccgcggaaag caactcacgg
     6181 tctctggctg attaccttgc ggagcagctc cgcaagcgcg gcgtaccctc gaagcagata
     6241 cacctattgc ccctcgtacg cggcgaagag aggtggaatg agctgtttgc agccgtggac
     6301 gaggctgatt tcctgttcct tgtgtttccg ctgttcgtgg atagccagcc gtggtcggtt
     6361 gtgcgtttca tggaacttgt cggtgcccac cggcgatcgt cgaacgccgg cgcgatgagg
     6421 ctggctgctc tggtcaacag cggttttccc gaggcacgcc acaacgagcc cgcacttgcc
     6481 atctgccgcc agttcgccgt agaatccaat atgtcgtggg ccggcggact ggctcttggg
     6541 agcggccccg tgatcggtgg gaagccgctt ccggacgcag gcagaaccgt gcggccagtc
     6601 atgaaagcgc tcgaaatggt agcggatgcg ctggccgaag gccgggatat ccctgctgag
     6661 gcaatcgcgt tgatggcaag accgctgatg cctcactggg tgtatcgaag cgtcgcgacg
     6721 ctcggattgt ggtggacggc ccgcaagaac ggcgtatccg ggaagctgcg cgcgcgtcca
     6781 tttacctcac ccgcggaacc ctgatggcga atacaggcga cactatccag cccggtgaca
     6841 tccgttacgc ggaggattcc atttgcccgg cagggtgcct cacccccgat gaggaactca
     6901 tccggaccgt cgccaatcga gttctcgccg cccttcagga taccgccgac ccggaatccc
     6961 ccaaactggt ccctgtcggc atttccgcac gccactgcca cgtcaccgag gaggtgctgc
     7021 agatcctcta cggccggggc gcgaagctcc atccctaccg cgagttgagg caacccgggt
     7081 tctacgccgc gcgggaaacc gttacggtgc tggggccgag gatgagagcg atagaaaacg
     7141 tgaggattct cgggccaacc cggacataca cgcaggtgga gctttcccgc accgacggtt
     7201 ttctgctcgg gctctccctt ccgatccgtg acagcggcga cctggagggt acccccggtg
     7261 tgacactggt gggcccggcc ggcagtatca cattgcccaa cggcgcaatg agagcgggac
     7321 ggcacgtgca catgtctccg gaagttgccg cggcatgggg gttgaccgac cggcagatcg
     7381 tccgcgccga gttcgagggg gagaagggcg ttgtgttctc caacgtcctc atccgcgtgg
     7441 gcaaggggca ggtgcttgag atgcaccttg atactgacga tggcaacgcg gccgaccttg
     7501 ttacgggtga catggtgcgt ctgttctgag acatccgccc gccttattcg ttcaactgcg
     7561 atcgcgagtg tgccgggaac ctttccgggc gcaccttcag atttacgttc cgggtgcagg
     7621 ctgcggcccc tgttttcgtt ccgtggccgt acccgagcca gtcgtttcca gacaaggcgt
     7681 cgcgcgccgg tgcgtggata accgaacgcc cgacgcgcca ggaatgtaac atgcggaaga
     7741 aagctctcat aaccgggata acaggacagg acggcgcata cctgagtcgg ctgttgctgg
     7801 aaaagggcta cgaagtccac ggaatcaagc gccgctcttc gcagttcaac accgcaagaa
     7861 ttgacgatct ctatcaagat ccgcacgaga gcgacaggag gttgttcctt cactacggtg
     7921 acgtaacgga cgcgctccac atgttccaga tcgtgaaaaa cgtacagccg gacgagatct
     7981 acaaccttgc cgcccagagc cacgtggccg tgtcgtttca gactccggag tacaccgcca
     8041 acgttgatgc aatcggcccc ctgcgcatac tggaagcaat cagaaacgct gatctggtgg
     8101 ggaaaacccg gttctatcag gcatccacgt ccgagatgtt cggcatggtc caggaaaccc
     8161 cgcagaccga gaaaaccccg ttctatccgc gctcgccgta cgccgcggcg aagctgtacg
     8221 cgtactgggt cacggtgaat taccgtgagg cgtacggcgt gtttgcgtgt aatggcatcc
     8281 tgttcaacca tgaatccccc atgcgcgggg aaacgtttgt gacgcggaaa atcacgaggg
     8341 ccgtcgcacg cataaaacac gggcttcaga aatgccttta cctcggcaat ctggacgcat
     8401 tgcgggactg gggccacgcg cgtgatttcg tcgaggcgat gtggctgatg ctgcaacagg
     8461 aacgccccga cgatttcgtg attgctaccg gcgaacagca ctcagttcgc gagtttgtcg
     8521 ttctggcgtt ccgcgaggtc ggcattgaaa tcgaatggaa aggcagtgga gacaatgagc
     8581 acgccattgc caccggtccg gtgtgcgaag ggcgttctcc tgatgacaac atcgttgttg
     8641 ccgtggatcc ccgttaccgc aggccctccg aagtggagac tcttctcggc gacgcaacga
     8701 aagccagaac gcaacttggg tgggtgccgc gcaccacgtt caacgaactg gtgaaggaaa
     8761 tggtggcggg cgacgttgaa ctggtcgagc gtgaccttct ggtaaggcgc cacgggtact
     8821 ccacgccgga ttaccacgaa tgagccgcac gaagctcgat cacgtgcaga ggaaggaggc
     8881 ttgacgacat ggcgatccac accacgccgc cgttcatgcc gacggatgcg aaaatctacg
     8941 ttccgggcca ccgcggcctt gtcggttccg cgatcgtccg tcgtctggaa agcctcggct
     9001 atcgcaacct gatcctgcgc acccgggccg aactaaacct tctggatgcc cgtgcggtgg
     9061 caagcctttt cgcggaggag aagcccgaat tcgtgttcgt ctgcgccgcg aaagtgggcg
     9121 gcatcatggc aaacaacacg tatcccgccg agtttttcta cgaaaacgtg gcgatagaaa
     9181 ccgccataat tcacgaggca tggcgcaaca acgtccggcg cctgctgttc ctcggctctt
     9241 cgtgtatcta tccacgggat tgcccgcagc cgatccgcga ggaatacctc atgacgggtc
     9301 cgctggaacc gacgaatcgc gcctatgcgg tggccaagat cgccggtatt gaaatgtgcc
     9361 gcgcatacaa ccagcagtac ggcacgcggt acctcgccgc aatgccgaca aacctgtacg
     9421 ggcagggcga caattacgat atccagacct cgcatgtact gccggcgctc atccgaaaaa
     9481 tgcatgaggc gaaaatccgc ggtgatgcca gtgtcacgct ctgggggaca gggcgcccgt
     9541 accgggagtt cgtgtacagc gacgatctgg cggatgcgtg cgtgtttctg ctgaacctcg
     9601 atgacgaacg gttcgggatg ctgctcggag atggctcttc aaatccgctg atcaacgtcg
     9661 ggtgcgggga agaccttacg atcgccgacc ttgccggcct cgtgaaagac gtggtggggt
     9721 tcgagggcac catcgtctgg gacacgacaa aaccggatgg taccccgaga aaactgcttg
     9781 acgtgagcaa actgaacggg ttgggctggc gaccttccat tcctctgcgg gacggaatca
     9841 ccagagcgta ccggcaattc ctcgaacacg aaaacaacgc gcgcgggcgt tcatgacgac
     9901 cgccggcccg tcagcacggg tatgaggggt tcaacgacct gtggactggt acggtataat
     9961 caagacgacc gtcggtatcc tggcgattgt gaatccgctg ggagcggtcg gaaccttcct
    10021 cagcgccacg gaaaaccttc cggccgccgc acggcggaaa accgccgaca ccgccgcagt
    10081 caccgtcacg gtaacactcc tcgtagcggc gtgggtgggc gaaacgatcc ttcacttctt
    10141 cggtatcagc atccccgcgt tccgcgtcgg cggcggcatt cttgttctca gcatggcaat
    10201 ctccatgctt cacgcgcggc catcacccgc caaacaggtt cctgaagaag cggcggatgc
    10261 agttgaacgg caagcagtcg gggttgttcc gctgggcatc cctctcatgt caggtcccgg
    10321 tgcgatcgcc cttgtgattg tggaatctca ccgagccccg acggtgccgg agcgtctcct
    10381 cgtgagcctt tgcatcgtgc tggttgccgt tgtgtcgtgg acgattctcc gcctcgccgg
    10441 actgttggcc aagcgtctca cccgcaccca catcaacatc tcgactcgtc tgatgggcct
    10501 tatcctcgcc gccatcgccg tggagttcat tgccagcggc gcgctggtcc tcttcccggg
    10561 gttgcggtag gcctcctatt ccaccgcgaa cgtcacgctc accactgccg tcacatcctt
    10621 ttcgcgggac gacgtgtcgt tgattccgta atcgctcacc atggtggagt tccgcggggt
    10681 gatctggaac acacccatgc gtgcgctccg gactgctcct atcctggagc caaccgcctg
    10741 cgcgatggtt tccgcgcgct tgcgggcgtc ctgtgcggct tccgcaagca tctccacgcg
    10801 cacgcttgcc agattcgagt acacgaactc gggtggattg gaggaaatcg gcacgccttc
    10861 tttcatgagg tccggcgcgt cagccgaaag ccgggtgatc cggtcaacgt cctccgactg
    10921 aatctcgaac ccctggaata tctggtatgc gaggatgcgc ccggtcatat gcccgccgga
    10981 aagcacttcc tgaatctgat tcgtctgcat cgcctgaaat gtgacagcgc tgtcggggat
    11041 tccctgctca cggaagtatg cccgaacgcg ttccgagtaa cgcttcacgt ccccgtacgc
    11101 ctcctgcatg gagggcttct gggcggagac gctcccgcgc cacaccaccc gatccgatgt
    11161 aattgccttc ttcgcggagc cggttacggt gatggtgtct cccgcccgtt tcgcgcggtt
    11221 cacggccgcg tatcccacga atgacgcaat caccagcgcg agtgccagcg caagcatccc
    11281 ggcgaattgc tgaggaaact tctccgacat ggcgcctcct tcctttgggt gagattgcct
    11341 acgcagaata taacgacgca ttccggacct tcagaacgtg gcgcgtccca gaccccggga
    11401 aaagaagaag gggcaaggtt gaacccgccc cttctccgcg taccggttgg gttcttccgg
    11461 ctcagcccag ccgaatgcgg atgcctgcga tttcccatgg ccggaacgaa cggcgtaccg
    11521 ttccgccctc catgttcaac cgcacgcccc tgttccattc catcagatcg gtgtcatacg
    11581 cctcgacaac aggacggtcg aacgtgatcg tcacgtcggt tgccgcgcct gtggtttcgt
    11641 acaggtgaag cacccagtcg tcgttgtcct ccgccttctt cagcgc
//
LOCUS       DAOTNG010000065        11661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5241_length_11661_cov_5.411339, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000065 DAOTNG010000000
VERSION     DAOTNG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5241_length_11661_cov_5.411339"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="P5179_04720"
     CDS             complement(<1..211)
                     /locus_tag="P5179_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRS94557.1"
                     /translation="MPKFVTKTISLGRTGDNELRDLIAPDKRIGKLVPVDEITPNPDQ
                     PRRHFDPGTLEALKESIRQHGLINPI"
     gene            complement(196..996)
                     /locus_tag="P5179_04725"
     CDS             complement(196..996)
                     /locus_tag="P5179_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRS94558.1"
                     /translation="MPPKLNRSPILCFSHIKGGVAKTTCCVNVGAGLADMGYRTLLVD
                     MDPQSSLTDVFLGNSEPPAASLYQVLYADGPGQRQMAISDIIRPVRENLDIAPASLDL
                     VRLEHLLLPEVDGRDRLRRALRGHGYDFVIIDTSPSVGILTSNAMIAATDLIIPVQAS
                     YFALKGIENIMETFNTIRERVNESLNLLGVVITMVDKRKRHPREVIADLVEIFPRELI
                     FDTQIREVAKVEASPGYHQDIFSFAPESPGADDFRAFIQEILTRCRNS"
     gene            complement(1616..2413)
                     /locus_tag="P5179_04730"
     CDS             complement(1616..2413)
                     /locus_tag="P5179_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS94559.1"
                     /translation="MAEKTLKGRTALITGSTSTGMGRSIALRLAAEGANIVLNYGTHR
                     DFSTHDGGADAQARAEEVASHIRRLGGQAIIAPGDTRSEEDTIRMVDAAKKAFGAIDI
                     LVLAAGGKWKPQPLESVTIDFWRDVMAAEVDAMFLAFKHVIPGMRARKFGRIIVFSMN
                     GAMTRKTLEATAADYTIGKTARTWLSLAVGHDEFENGITVNIIEPGPVGHMTLNEALA
                     AIEHTDNAWEQRAKPVAHDAAELAAFLCSEAGRFVSQSLIRYPTDGW"
     gene            2591..>4011
                     /locus_tag="P5179_04735"
     CDS             2591..>4011
                     /locus_tag="P5179_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94560.1"
                     /translation="MTRIRLPFLRGTAYGVRGAAKITFFVACLLGAVSTAGAQYRRDP
                     SAVELGEIGSRHAAQIDSARFVRSWELLSRYPTREIGTAGNEAATDYIAATFDSIGMQ
                     HVTRQRVTVADARQQYAYAVLPLEDGTFDTIPLRCYAPNFAHTNTIPPGGLKGHLVYS
                     RTGEAREYNGHEMEGSVLLAEMTVEERWRLASTLGSKAAIFIEAPKVTISAQFYTSVN
                     FPRFWVNRQDGDSLRAWAAAHPNTEITIHAKQPWRMLTGNSVTGFLPGSDPVLSREVV
                     IITAPFDAGSNVPGLAPGAEAAFSSAAQLELARIFGKERPPRSLYFAALNGQRSGVME
                     GGRVFVQALKQSYPGALRQNLERLRADLRLISDRKLSGRLPAHRERALALERRLGDVV
                     GNELATAQRYWDDLQNARTEYLKGGAVAEKRTGGALYYARRVRVGAMEEAAERFKPAF
                     KRALDACEPLIADYGRLLEDFVG"
     assembly_gap    4012..4111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4112..8206
                     /locus_tag="P5179_04740"
     CDS             <4112..8206
                     /locus_tag="P5179_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRS94561.1"
                     /translation="DLSLTSNSNNIGLFHKGYFYNQQPDLELQRDYGPLGKRFMGYAT
                     NLVVVNDLNGLWSRSDEQIRALILAKIAEISAKAETYRKVAGIALSDTEVNYSYDWYQ
                     RFVNATAWAWGLLLAGVCAWFAYRRRRADETSIIPAWWQIAGIVAGLGLTGYALIAGM
                     DWKNTEKIHHGIDQLIWNHTDLLINAEQIPREEKTKIRLAAERHKRYVEMVEAPEFFA
                     ENIINAVPATLNKLRQDSLIIAQQLVMFQKIAPWRTIPPAQLTQGQLDTLNQFIGFLG
                     LQGDRRFVDGVAGTPGKAWWNFLPAARYFTSEIASLAGIPAIALSTTGDIAPELSSLR
                     DTFSRVNIPGSLTQMKTLTAITGQALQDPLLPDDMVMGDYFATSSGLVLYDDRQASVT
                     ADQPVDSAVVIADRGGYRFTTITNRRGAFEFVGLPLDGRAAARSADPQWLHAYKTSAD
                     SGNVRFAIDLGSKQYAFVPSVSQLSQDWTVVVFPCASVSVTDVLDPRYFQFLARGDIY
                     EARTDSKPWHYFSHVAMGDNTFFVEEGTRVKIIFAQGLLGVRMMLTGTPDSIQIPEHR
                     QGLGFKVEREALLTHTAYQAARDMWQLNEGRLVTLVRHGIIDAETRAVHDSSAYRLAL
                     AKKALEEKRYDTYLTEARSALALESRVLPDVLGIANGALKGVLFYMALLLPFAFFVER
                     LIIGSTDVKKQAIWFFAIFILMFVLIQQVHPAFDITLAPPMVLLAFTVFALSLFVVSI
                     IIGKFTAQMKQIKKEQQGYLSADVGRVSAMFAAFLLGVSNMRKRKVRTTLTCLTLILL
                     TFTVLSFTSVQELLRVNKVRLSAQKPTYSGVLIRDRFWNFWPRETLTSIRNEFGQENI
                     VVGRGWREALIQGATSNLFTLSAPNGKSYTLSGFVGMEPQEDEVTGIFRKTGVAGRWF
                     QPGDFRVLVIPQTAADALGITRDQVTGDEDTAPQIIMDGVRYGVIGIVDDELLRQLKD
                     IDGEELTSVNWALVETKRQDRISQVVQAQKAPTAGERDSFIHLVPAQCMFMPLNLLPG
                     GLVSVAIKTPDSNTALGLVNRVIPKWALEMYVGQNEGTFLYSAIGLTTLGGLKDVLIP
                     ILIAALIVLNTMLGAVFERTREIGIFSSVGLAPSHIAMLFMAEAVVYAVLGAIAGYLL
                     GQTVGQVIYKTGLLGGVTLNYSSLSAIVSTLIVMITVILSTIYPAKKASEISVPDIAR
                     TWKLPEPNGDEWFFALPFTLMDEELEGVNAFLAHFFESHRDESASDFYIDRVDFWADS
                     GRFMLETMVWLAPYDLGVSELVTLISSPVPNEKGLFGMSLLVTRESGDVPSWNRVNRR
                     FVNYLRKQFLIWRTLTPAEREMFNERGRAMYSVNGERAISQKTT"
     gene            8263..10317
                     /locus_tag="P5179_04745"
     CDS             8263..10317
                     /locus_tag="P5179_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94562.1"
                     /translation="MADADNDRGFWKKLTGRGRAEGDRSTQEDKLAAERKKYGMDPEL
                     EMAPYESGLTARTLIGALFVAIVMMPASIYLGLVVGTGLGSAAEWVSIILFTELARRS
                     FMPLKRQEIYILFILAGGLVGGGPFGGLIWNTYLQSSQEALSLGVAQRLTTENIWWAV
                     PPAGSEAVALRTFWHKDWLPIVTLMVFTSLISRFQTLSSYYFLFRLTSDVERLPFPFA
                     PIAAQGITALAEGSEGETWRWRWFSIGSMIGLVWGAIYVGVPTVTAGIFGTAIEILPI
                     PFVDFTREIGNVMPGSLLGFNTDLGGIMWGFVVPFPIICGQVISAFLSSFVLPPFLVE
                     AGILKSWRPGLGVVQASVLNNLDLWTSVSLGVGLPIMFLGVYESTKVLMRQRDGRGAG
                     TFAPPPGRGDLPLWLAAAIWFVTSAFFVGLAHWLVPLFPVWIYILFAFVYTPFISYIG
                     ARMIGHAGTHVAFPPITQAAFILSGYKGIAVWWVGSQANDFSGGVSKFREIELTKTKF
                     TSYFAVEFIMFPITFILSFAFWSVIWKMNPIPSSAYPFALKMWPLNAYNACLWQTATI
                     QGNSTMLNAIKFDVIGYAAIGGGVLYAIIHFLHASPMWWYGLIGGLGAWPHGIFLSLT
                     GALLSRYYLAKKFGGPEQWFKYAIVLNAGYACGVGLIGMAGVAIALINGAITQSPFQV
                     FR"
     gene            complement(10345..11409)
                     /locus_tag="P5179_04750"
     CDS             complement(10345..11409)
                     /locus_tag="P5179_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94563.1"
                     /translation="MKSALWVLLGSLLPFAVQAGSFSGEIRTLGGYDSNPSGYTGGEA
                     QGLGATQAKLTWRVPLGDASGDFRVTYLGNLSAFTPRSEWTAHSHELSGAFSQDLEDN
                     LNVGFRGGAGGNWNRPAYDAYSYREFFGEGWAQTELDGWPVYASLAGNYRNYPASPSF
                     DFREFTMTAYTSRYWATRTTFRLTFSATERTYITQTIEDFFAGQTNATSSQFQISALA
                     SQGLTDRSGLRFSGWALSGSGRTRWRDDYLEAMDDPLATRGVGGRVQFSVLAPLSLTF
                     RTYLGGQRTTQAYITQTGEYGDHTDTIREAGVVLEGKLPRISTRLSWSTELNAQRRTS
                     SDPTYAFDRAALTVGVKYAW"
BASE COUNT         2137 a         3306 c         3696 g         2422 t
ORIGIN      
        1 tgatcgggtt gatcagcccg tgctggcgta tactttcctt caacgcttcg agtgtgcccg
       61 gatcgaaatg cctgcggggc tggtcggggt tcggtgtgat ctcgtctaca ggcaccagtt
      121 tgccgatacg tttgtcgggc gcgatgaggt cccggagttc gttatcgccg gtgcgcccaa
      181 gggagatggt tttcgtcacg aatttcggca tcgcgtcaga atctcctgga tgaacgcgcg
      241 gaaatcgtcc gcgccggggc tttccggggc gaaggagaag atatcctggt ggtagccggg
      301 gctcgcctcg actttggcga cttcccggat ctgtgtgtcg aaaatcagtt cgcgcgggaa
      361 tatctccacc aggtcggcga ttacttccct cgggtggcgt tttctcttgt ccaccatggt
      421 gatgacgacg ccgaggaggt tgagcgactc gttcacccgc tccctgatgg tattgaacgt
      481 ctccatgatg ttctcgatgc ctttgagggc gaagtaactc gcctgaaccg gaataatgag
      541 gtccgttgcg gcaatcatcg cgttcgacgt caagatacct accgaagggc tcgtgtcgat
      601 gatgacaaag tcgtacccgt ggccgcgtag ggcccggcgg agccggtcgc gcccgtccac
      661 ttccggcagg agaaggtgct caagccgcac gagatccagg gatgcgggcg caatatcgag
      721 attttctcga acaggccgga tgatatcgct gatcgccatc tgtcgctggc ccggcccatc
      781 ggcgtacagc acctggtaca acgaagccgc cggaggttcg ctgtttccca ggaacacatc
      841 ggtaagcgag ctttgcggat ccatgtcaac gagcagggtt cgatacccca tatcggcaag
      901 gccagccccg acattgacgc agcacgtcgt ttttgcgact ccgcctttga tgtgggagaa
      961 acacagaatc gggcttcggt tcagttttgg cggcatggcg tgggtccctg tgcgcggaaa
     1021 cgatctactg gcgtgtacgg atcactgccg gcaggaaacg ggatttcacc gacctgctgg
     1081 ccgcttggtg gggaggttcg gacgcgcatg atttcccggt ggcgctcgtg attcagcact
     1141 gatttcccgt gggaattgtc gtccgattgt accgcgctac cgcggaagca gtcaaggggt
     1201 tgcttgtccg gcgggccatg cagggcaacg ggcaagttgt ttctctgctg taccaagagg
     1261 gggacaggct gcgactccgc ggtttacata acactgataa tccagtattc gcttcacgcc
     1321 gggacgtaaa ggggcaaaag tcaagggctc cacacggtgc ggcaggtatt tcggcaaggc
     1381 cacagaggag gacgatcacg gcgaccacag gccgtgatcg cactgatgtc gttgtatccc
     1441 actgtatcga atgttaagcc agaacagggt gattgtcagg cgaggtgctc ctttccgagg
     1501 cagaatacgg acaaaacccg atagctacgg ataacataac agacgtggcg caacgccggg
     1561 agaagtcgcg ccacgtcgtg gttggtgtcc ctggtttatc gcaggcgtgt tcttctcacc
     1621 atccgtcggt cggatagcgg atcaggctct gactgacgaa tcggcccgct tcggagcaga
     1681 ggaaggcagc cagttcggcc gcatcgtgcg caaccggctt tgcgcgttgt tcccatgcgt
     1741 tgtctgtgtg ctcgatggcg gcaagtgcct cgttcaatgt catgtgaccg acggggccgg
     1801 gttcgataat gttgacggtg attccgtttt cgaattcgtc atgaccgact gcgaggctga
     1861 gccaggtgcg ggccgttttc ccgatcgtgt agtccgcggc cgtcgcttcg agggttttcc
     1921 gcgtcatggc gccgttcatg ctgaatacga tgatgcgtcc gaatttccgc gcgcgcattc
     1981 cgggaatcac gtgtttgaac gcgagaaaca tcgcgtcaac ctcggccgcc ataacatcgc
     2041 gccagaaatc aatggtgacg gactcgagcg gctgaggttt ccacttgccg cctgctgcaa
     2101 ggacaaggat atcgattgcg ccgaaggctt tttttgccgc gtcaaccatg cggatggtgt
     2161 cctcttcgct tcgggtgtca ccgggcgcga tgattgcctg tcctccgagg cggcgaatgt
     2221 gggaggccac ttcctccgcg cgggcctgag catccgcgcc accgtcgtgg gtggagaaat
     2281 cccggtgagt gccgtagttc aggacgatgt tcgcgccttc ggccgcgagg cggagggcga
     2341 tgctgcgacc cattccggtg ctggtgcttc ccgtgatgag tgcggttctg cctttgagcg
     2401 ttttttctgc catggaagtt cctcctgtgt gtgtgccctc cataagatcg ttcgcggggg
     2461 tgcgcgaaaa aaggcgtttg tctgaaaacg gaggctcgac gcagtgccgc ggtcttgtta
     2521 ttttctcaga ttcggaatcg ggatgcatcg atctcaccgc tgttttgcca cgttaatcct
     2581 cggatcaacc atgactcgta tcagactgcc gtttctccgt gggactgcgt acggcgtccg
     2641 cggggcagca aagatcacgt ttttcgttgc gtgtctcctg ggcgctgtct caactgccgg
     2701 cgcgcagtac cgtcgcgacc caagcgcagt ggagctcgga gagatcggtt cgcggcacgc
     2761 cgcgcagatc gattccgcgc gcttcgtgcg ttcgtgggag ctgctctcgc gttatccgac
     2821 gcgggagatc ggcacggccg ggaacgaagc cgcgacggac tacattgccg ccacgtttga
     2881 tagcattggc atgcaacacg tgacccgaca gcgggttacg gttgcggatg cgcggcagca
     2941 atatgcgtat gcagttcttc cgcttgagga cggcaccttc gatacgattc cactgcgctg
     3001 ttacgcgccg aatttcgccc acacgaacac cattccaccc ggaggattga aaggccacct
     3061 cgtgtacagc cggaccgggg aggcgcggga atacaatggc cacgagatgg aaggaagtgt
     3121 tcttctcgcc gagatgacgg tggaagaacg ctggaggctt gcgagcacgc ttggaagcaa
     3181 agcggccatt ttcattgaag cacccaaggt aaccatatca gcgcagttct acacctccgt
     3241 aaacttccct cgtttctggg taaaccggca ggacggggat tcgttgcgtg cgtgggcggc
     3301 agcgcacccc aacacggaga tcacgataca cgcgaaacag ccgtggcgta tgctgaccgg
     3361 taacagcgtg acggggtttc tgccgggctc tgacccggtt ctttcgcggg aagtcgtgat
     3421 tatcaccgcg ccgtttgacg caggatcgaa tgttccgggt ctggcccctg gcgccgaggc
     3481 ggcgttcagc agcgcggcac agcttgagct tgcgcggatt ttcgggaagg agcggccgcc
     3541 acggtcgctc tattttgcgg cgctcaatgg ccaacgaagc ggtgtgatgg aggggggacg
     3601 cgttttcgtt caggcgttga agcaatcgta cccgggggcg ctgcggcaga atctggagag
     3661 attgcgtgcg gatctcagac tgatcagcga ccgcaagcta tccggccggc ttcctgctca
     3721 tcgcgagcgg gcactggcgc ttgagcggcg gttgggagat gtggtcggga acgaactggc
     3781 aacggcacaa cgctactggg atgaccttca gaacgcccgg acggagtatc tgaagggcgg
     3841 cgcggttgcc gaaaaacgta cgggcggagc tctctattat gcccggcggg tgcgtgtcgg
     3901 agccatggag gaggcggctg agaggttcaa accggcattc aagcgggcgc tggacgcgtg
     3961 tgagccgctt atcgcggact acggccgcct tctcgaggat ttcgtcgggg annnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacctgtcg cttacgagta attcgaacaa
     4141 catcggcctc ttccacaagg ggtacttcta caaccagcag ccggatctgg agcttcagcg
     4201 cgattacggg ccgcttggaa agcggttcat ggggtatgcc acgaacctcg tcgtggtaaa
     4261 cgatctgaac gggttgtggt cgcgctcgga tgagcagatt cgggcactga tcctggcgaa
     4321 gattgccgaa atcagcgcga aggcggaaac ctaccggaag gttgccggaa tcgcgctgag
     4381 cgacacggag gtgaactact cgtacgactg gtaccagcgg tttgtcaacg cgaccgcatg
     4441 ggcgtggggt ctgttgctcg cgggtgtctg cgcgtggttt gcgtaccgcc gcagacgcgc
     4501 tgatgaaacg agcatcatac ccgcgtggtg gcagatcgcc ggcattgtgg cgggactggg
     4561 tcttaccggg tatgcgctga ttgcgggaat ggactggaaa aacacggaga agatccatca
     4621 cggcattgat cagcttatct ggaaccacac ggacctcctg atcaacgcgg agcagattcc
     4681 gcgggaggaa aagacgaaga ttcgtctggc ggcggagcgg cacaagcgct acgtggaaat
     4741 ggtggaggcc ccggagttct tcgcggagaa tatcatcaac gcggtaccgg ccacgctgaa
     4801 caagctgcgg caggacagcc tgattatcgc ccagcagctt gtcatgttcc agaaaatcgc
     4861 gccgtggcga acgattcccc ccgcccaact gacacagggg cagcttgata cgctgaacca
     4921 gtttatcggt tttctgggcc ttcagggcga tcgccggttc gtggacgggg tagccggcac
     4981 accgggcaaa gcgtggtgga atttcctccc cgcagcgcgg tatttcacga gtgaaatcgc
     5041 gagcctggca ggaattcccg ccatcgctct ttccacgacc ggagatattg cacccgaact
     5101 atccagcctg cgtgatacgt tttcccgggt caacatcccc ggcagcctga cgcagatgaa
     5161 aactctgacc gcgatcacag gtcaggcact tcaggatccg ctcctccccg atgacatggt
     5221 catgggcgat tacttcgcca cgtcttcggg cctcgtgctg tatgacgacc ggcaggcttc
     5281 cgtgacggcg gaccagccgg tagacagcgc cgtcgtgatt gcggatcgag ggggatatcg
     5341 cttcaccacg atcaccaacc gccgcggcgc atttgagttc gtcgggttgc ccctcgacgg
     5401 gagggccgca gcgcgctccg cagatcctca atggcttcac gcctacaaga catcggcgga
     5461 tagcgggaac gtccggttcg cgattgacct tggcagcaag cagtacgcct tcgtgccgag
     5521 tgtttcgcag ctatctcagg actggacggt tgtcgtattc ccgtgcgcgt cggtgagcgt
     5581 gacggacgta ctcgacccgc ggtatttcca gttcctggcc cgcggagata tttacgaggc
     5641 gcgcacggat tccaagccct ggcattactt ctctcacgtg gcgatgggag acaacacatt
     5701 tttcgtcgag gagggcacca gggtcaaaat catcttcgcg caggggcttc tgggcgtgcg
     5761 gatgatgctg acgggaaccc cggacagcat acagatcccc gagcaccgcc agggactggg
     5821 cttcaaggtt gagcgcgaag cgctgcttac gcatactgcc taccaggcgg cgcgggacat
     5881 gtggcagttg aacgaggggc ggctcgtcac gcttgtccgc cacggaatca tcgacgcgga
     5941 gacccgggcc gttcacgact catcggcgta tcgtctcgcg ctggcaaaga aggcgctgga
     6001 ggagaaacgg tacgacacgt atctcaccga ggcacgctcc gccctcgcgc tggaatcgcg
     6061 tgtgctgccg gacgtactgg gtatcgcgaa cggcgcgctg aagggcgtgc tgttctacat
     6121 ggcgctgctg ttgcctttcg cgttcttcgt ggaacgcctc atcatcgggt ccacggatgt
     6181 gaagaagcag gcgatctggt tcttcgccat cttcatcctg atgttcgtgc ttatccagca
     6241 ggttcacccg gcgttcgaca tcactctggc gccgccgatg gtgcttctcg cgttcaccgt
     6301 gttcgcgctc agtctgtttg tcgtttcgat catcatcggc aaattcacgg cgcagatgaa
     6361 gcagatcaag aaggagcagc aggggtatct ttcggcggat gtagggcgcg tgagcgcgat
     6421 gttcgcggca tttctgcttg gtgtttcaaa catgcgaaaa cgcaaggtcc ggacgacgct
     6481 gacctgcctc acactgatac tgctgacgtt cacggtgctg tcattcacga gcgtgcagga
     6541 gctcctgcgg gtgaacaagg tgcggctgag cgcgcagaaa cccacgtaca gcggtgttct
     6601 cattcgagac cgattctgga atttctggcc gcgagagacg ctcacctcca tccggaacga
     6661 attcggccaa gagaacatcg tggtgggccg tggatggcga gaagcgctca ttcagggtgc
     6721 aacgagcaac ctgttcacgc tgagcgcacc caacggcaag agctacactc tgagcggatt
     6781 tgtaggcatg gagcctcagg aggatgaggt aaccgggatt ttccggaaaa ccggcgtggc
     6841 ggggcgctgg ttccagccgg gcgatttccg cgtgctggtg ataccgcaga cggccgcgga
     6901 cgcgctcggg atcacacgcg accaggtgac gggggacgag gacacggcgc cgcaaatcat
     6961 catggatggg gtgcggtacg gcgtgattgg cattgttgac gatgagcttc tccgccagtt
     7021 gaaggacatt gacggcgagg agctgacttc cgtcaactgg gcgctggtgg aaacaaagcg
     7081 gcaggataga atcagccagg tggtgcaggc gcagaaggcc ccgaccgccg gagagcggga
     7141 ttccttcatt caccttgtgc ccgcgcagtg catgttcatg ccgctgaatc tcctgcccgg
     7201 cgggctggtt tcggtagcca tcaaaacccc ggacagcaac accgcgctgg ggctggtgaa
     7261 ccgggtgatt cccaagtggg cgctggagat gtacgtcggc cagaacgagg ggacgttcct
     7321 ctacagcgcc atagggctta ccacccttgg cggtctgaag gatgtactga ttccgatcct
     7381 catcgccgca ctgattgtct tgaacacgat gctgggcgcg gtattcgaac gaacgcggga
     7441 aatcgggatc ttcagctccg taggcctggc tccgtcgcac attgcaatgc tgttcatggc
     7501 ggaagcggtg gtgtacgcgg tgctgggggc gatcgccggg tacctgctcg ggcaaaccgt
     7561 ggggcaggtg atctacaaga ccggccttct gggcggggtg acgctgaact actcctccct
     7621 ttccgcaatc gtctctacgc ttatcgtgat gattaccgta atcctgtcca cgatctaccc
     7681 ggcgaagaag gcgtctgaaa tcagcgtgcc cgatatcgcc cgaacgtgga agctgccgga
     7741 accgaacggg gatgaatggt tttttgcgct gccgttcacg ctcatggatg aagagctgga
     7801 gggggtcaac gccttcctcg cgcatttctt cgaatcgcac cgcgatgaat cggcgagcga
     7861 tttctatatc gaccgcgtgg acttctgggc ggacagcggc aggttcatgc tcgagacgat
     7921 ggtatggctt gcgccgtacg atcttggcgt aagtgagctt gtcacgctca tcagttcgcc
     7981 ggttccgaac gaaaagggtc ttttcgggat gagtttgctc gtcacacggg aatcgggcga
     8041 cgtcccatcg tggaatcgcg tgaaccgccg gttcgtcaac tacctccgca agcagttcct
     8101 gatctggcgt acgcttacac cggcggaacg ggagatgttc aacgagcggg gccgcgcgat
     8161 gtacagcgtg aacggtgagc gggcaatttc tcagaaaacg acgtgaggcg cgccgtctgg
     8221 tttccggtgt gcaggaatca acaggaagac gacaggtagg ccatggctga cgcggataac
     8281 gaccggggtt tctggaaaaa gctgacgggg cgcggtagag cggagggcga tcgctccacc
     8341 caggaggata agcttgcggc ggagcgcaag aaatacggca tggaccccga gctggagatg
     8401 gcgccgtatg aaagtggtct gacggctcgc actctgatcg gcgcgctttt cgtcgcaatc
     8461 gtgatgatgc cggcctccat ctacctcggt ctggtagttg gcacggggct gggctcggcg
     8521 gccgaatggg tttccatcat cctgttcacg gagctcgccc gacgcagctt catgccgctg
     8581 aagcggcagg aaatctacat cctgttcatc cttgccggcg ggctggtggg cggaggcccg
     8641 ttcgggggcc tgatctggaa cacctacctc cagtcatcgc aggaggcgct gagccttggc
     8701 gtggcgcaac ggcttaccac ggagaacatc tggtgggccg tgccgcctgc cgggtcggaa
     8761 gcggtggcac tgcgcacgtt ctggcacaag gactggctgc ccatcgtcac gctgatggta
     8821 ttcacgagtc tcatcagccg cttccagaca ctcagctcct actacttcct gttccggctg
     8881 acgagcgacg ttgaacgtct gcccttcccg ttcgcaccca tcgccgccca gggaattacg
     8941 gcgctggcag agggatcgga aggtgaaacg tggcgctggc ggtggttcag catcggctcc
     9001 atgatcggcc tcgtgtgggg cgcgatttac gtcggcgtac ccacggtgac cgcgggcatc
     9061 ttcggtacgg cgattgaaat cctccccatc cccttcgtgg atttcacccg cgaaatcggc
     9121 aacgtcatgc cgggttcgtt gctcggattc aacaccgacc tcggcggcat catgtgggga
     9181 ttcgtcgtgc cgtttccgat catctgcggg caggtgatca gcgcgtttct cagctcattc
     9241 gtgctgcctc ctttcctcgt ggaagcgggt atcctgaaaa gctggcggcc cggactgggc
     9301 gtggtgcagg cgtcggttct caacaacctc gacctgtgga cctcggtgtc gctcggcgtc
     9361 ggcctgccga tcatgttcct cggggtgtac gagtctacga aggtgttgat gaggcaacgg
     9421 gacggacgcg gcgccgggac gttcgctcct cccccggggc ggggcgacct tcccctttgg
     9481 cttgcggcgg cgatctggtt cgtaacgagc gccttcttcg ttggcctcgc acactggctg
     9541 gtgccgcttt tccccgtgtg gatctacatc ctgttcgcgt tcgtctacac gccgttcatc
     9601 agctacatcg gcgcgcgcat gatcgggcac gccgggacac acgtggcgtt cccgccgatt
     9661 acgcaggcgg ctttcatcct ttccgggtac aagggtatcg ccgtctggtg ggtgggttct
     9721 caggcaaacg acttttcggg tggcgtgtcc aaattccggg aaatcgaact gacgaagacg
     9781 aagttcacga gctacttcgc cgtcgagttc atcatgtttc ccatcacgtt catcctgagc
     9841 ttcgcgttct ggtcggtgat ctggaagatg aacccgatcc cctcgtcggc gtatccgttc
     9901 gcactgaaga tgtggccgct gaacgcgtac aacgcgtgtc tctggcaaac ggcaacgatt
     9961 cagggcaact ccaccatgct gaatgccatc aagttcgacg tgatcgggta tgcggcgata
    10021 ggtggcggcg tcctttatgc gattattcac ttcctgcacg cctcaccgat gtggtggtac
    10081 ggcctgatcg gtggtctggg cgcgtggccg cacggcattt tcctgtccct cacgggcgcg
    10141 ttgttgagcc ggtattacct cgcgaagaag ttcggcgggc cggaacagtg gttcaaatac
    10201 gccatcgtgc tgaacgcagg atacgcatgc ggagtcgggt tgatcggtat ggcgggagtg
    10261 gcgatcgcgc ttatcaacgg ggcgattacg caatcgccct tccaggtgtt caggtagcgg
    10321 ccggatcgcg tcagaggttt tcgcttacca cgcgtatttc acgcccaccg tcaatgcggc
    10381 gcgatcgaag gcgtacgtcg gatcgctcga ggtccttcgt tgtgcattca gctccgttga
    10441 ccacgacaac ctggtgctga tccggggcag cttcccttcg agaactacgc cggcctcgcg
    10501 gatggtgtcg gtgtggtctc cgtactcgcc ggtctgagtg atgtaggcct gagtcgtgcg
    10561 ctggccaccg aggtacgtgc ggaacgtaag tgacaacggc gccagaacgc tgaactggac
    10621 tcgcccgccc acgccgcgcg tcgccaacgg gtcgtccatc gcttcgaggt agtcatcccg
    10681 ccagcgggtc cttccggatc cgctcagtgc ccaccccgag aacctcagtc cgcttcgatc
    10741 cgtcaggccc tggctggcca gcgcggaaat ctggaactgg ctgctcgttg cgttcgtctg
    10801 gccggcgaag aaatcctcta tcgtctgcgt gatgtaggtg cgttcggttg cgctgaaggt
    10861 gaggcggaac gttgttcgcg tcgcccagta acgggatgta taggccgtca tcgtgaattc
    10921 gcggaaatcg aacgacgggc ttgcggggta gttgcggtag tttcccgcca gagacgcgta
    10981 caccggccac ccgtccagtt ccgtctgggc ccagccttcg ccgaagaact cccggtacga
    11041 ataggcgtca tacgcaggtc ggttccagtt tccacccgcg ccaccgcgga accccacgtt
    11101 caggttatcc tcaagatcct ggctgaaggc gccgctcagt tcgtggctgt gcgcggtcca
    11161 ttctgaacgt ggcgtgaacg cggacaggtt accgaggtac gtgaccctga aatcccctga
    11221 tgcgtcgccg agaggaacgc gccaggtgag tttcgcctgc gtggcgccga gaccctgcgc
    11281 ttcaccccct gtgtatccgg aggggttgct gtcgtacccg ccgagagtgc ggatctctcc
    11341 ggaaaacgaa ccagcctgca cggcgaaggg gagcagggat cccagaagca cccacaacgc
    11401 gcttttcatc gttccacctg aagggaagcc ggagcggcgc agcgccgccc cggttgtgat
    11461 cgtctaccat gcatgaatgc tcatgatgct cgcaagttgc ccgggttccg ggaggagagg
    11521 ttatcgtccc ctgggcccct tgccctgtgg cggcatggca acgccatact gattgaactt
    11581 cgcggcgagg gcgtcacgga tttccgccgc cgatctgcct tccgccctca tggttcgcac
    11641 gagagcctgt acctctgctt t
//
LOCUS       DAOTNG010000066        11628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5257_length_11628_cov_5.167891, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000066 DAOTNG010000000
VERSION     DAOTNG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_11628_cov_5.167891"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..444)
                     /locus_tag="P5179_04755"
     CDS             complement(94..444)
                     /locus_tag="P5179_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRS94564.1"
                     /translation="MRYLILRLLLTCLSIYIAARAVDGVALVEPWAWWHLLLVGAVFG
                     LLNAVVKPILTFFSLPVLILTMGLFYFVINALILWMTPVFVPMLVVDGFIAALKGSVV
                     ISLMNWLFSWLVGI"
     gene            complement(608..2053)
                     /locus_tag="P5179_04760"
     CDS             complement(608..2053)
                     /locus_tag="P5179_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94565.1"
                     /translation="MTARVRQTSRFAFRVGASEQDITPPYGAYLSGSACGDRRPAQSV
                     LDRTYARAIVFDDGRARVCVISLDVTIITGEYSTRIRNDVSDATGIPIEAIMVFAEQT
                     HSAPSVGRFMLDPDFPLETTPETEYLWGSETPYCEFASKKAVQAAIEAHDRLQPARVG
                     LARALNAEFSFNRRGVTRDGRIIMPKPAGRENQPFGITELCYMEGPMDPEVGVACFQG
                     LDMRPIAFLLHHTCHPVNVFGSRGTYRAVSSDWPGAWAEGMKRAYGSHVVPLVLNGCC
                     GNINPWNPFEPDARPDHRRMGKALAEVSEKLVHTMQFAEGAQVASCADCVSLPFREIP
                     GERRRMVAEILERSPEPPRSANGEVDPVWFTAASTRSVEILKQRETHMRYEMQVFRVG
                     DLAIVGLPGEPFVEGQLAIKTSSPVPFTFPTHMVSHYVGYLPTREAYARGGHEANNEV
                     TYWAKLALGCLETVAERAVVNIKTLFPDRVA"
     gene            complement(2073..2486)
                     /gene="nikR"
                     /locus_tag="P5179_04765"
     CDS             complement(2073..2486)
                     /gene="nikR"
                     /locus_tag="P5179_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRS94566.1"
                     /translation="MGGLARFSVAVGEDLLKRFDRLIRESGYSNRSEALRDLIRDRLV
                     AEQWAAGEEVVATITLVYDHHVRDLAERLTEVQHQRTDRIISSLHVHLNHDNCLEVIV
                     TRGSAHDVKELAGLLIGSRGVKHGGLVMTTTGEEL"
     gene            complement(2500..3630)
                     /locus_tag="P5179_04770"
     CDS             complement(2500..3630)
                     /locus_tag="P5179_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRS94567.1"
                     /translation="MLLPFDKDDTTMTRADDIALKRVRVRDFLRSHDLGGVLFTQQKS
                     FSWYTSGGQNHVGLTTEAGAAHLLATPDQDYLVANNIEAPRIMDEEGLSELGIQPLVY
                     QWDSPETDATRLVERVLAGQRYATDTGQFAGELNHLRYSLTPVEVERYRALGKDCAEV
                     MSDLCLAVEPGQTEWEVAAALSKRLWEREIVPAVVLVAADDRISKYRHPIPTANKIAK
                     YVMVVMCGRRHGLILSTTRLVHFGKLPAELKRKHNAVMRVDAAYIGATLIGADVDEVF
                     QKGLDVYEETGFKDEWMLHHQGGGTGYASRDFKGSLHCKERVQAWQAFAWNPSITGTK
                     SEDTMIATPDGPEILSMIRDWPSVEVPAGLWGSLRRADILVR"
     gene            complement(3833..4927)
                     /locus_tag="P5179_04775"
     CDS             complement(3833..4927)
                     /locus_tag="P5179_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94568.1"
                     /translation="MHFVECDTVSGAEIYQLTDGPRPADNIYGEQPYSSPCGTRIAVR
                     YYPHEGTDGGLSILDLVDGSLHPVLKKMPRFPAFHAWGEYLYFQEYVGDEMLLRRCHY
                     VNTTMEDVLVLPATGSQLSYGTVSPDVEYYAVNSHPDSTSSRLLLFDLVDGVHSVLAE
                     SSAQWFKHEQFSRDGRDAILIQANSADVSVVNLGVVYPCGGEIEWLPVDSRPLVPVGK
                     WPGRDRYTPRCTGHEAWIGKTDRVFLSTAYDSSSMTNVWSVSLSDEQPAAACVGEHVF
                     GHVATSGCGRFWIADAPSEEGIPIYIGSFVTGSNSRLCFSRTVHDGQQWSHTHPYFTA
                     DNRWVIFTSNRSGIPQIFGARLADGFLSSL"
     gene            complement(4980..5606)
                     /locus_tag="P5179_04780"
     CDS             complement(4980..5606)
                     /locus_tag="P5179_04780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS94569.1"
                     /translation="MSVQPLRTAEITADPMCAAMELRLPSGEHVVFRPLRAEDATLLG
                     EYFTSLTRETTRFYGPHAFDQATADQLCGSINYDDTIRFVALTRVDGREQIVAYFILV
                     LGTSESDGKRYADVGIQLDPAQDCTFAPSVREQFQNQGLGTPLFRHVIHVARRLGRRK
                     MVLMGGVQVLNTRGIHFYEKQGFRRVGGFEGPPGIFSQDMELDLLLDS"
     gene            5870..6706
                     /locus_tag="P5179_04785"
     CDS             5870..6706
                     /locus_tag="P5179_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS94570.1"
                     /translation="MASTIRVAMIQRAFVPFEREANISIAERAIRAAARDGAHLVALP
                     EMFLTGYPHAPAKDSADWPQFVERLYGTAETADGTSARKLGEIAKDAGVVLIAGICEI
                     RDGRLFNSAFVWDSKGEHIGTYCKVHTCRFTALEDLCEDGDDWHVWPLTVGDQLVRVG
                     VMICYDREHPESARVLMLKGADLIIVPNACGIDERRLIQLRTRAYENVVYIGMANHSK
                     PHNGQSVITNYWGDILTQAGEGDETIFADLDLEALRDERTHTIWGNNFRRPWKYGLIA
                     KT"
     gene            6771..8081
                     /locus_tag="P5179_04790"
     CDS             6771..8081
                     /locus_tag="P5179_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94571.1"
                     /translation="MKSFVSRAHGVATNSLRRWNAQSVDFRWFILALCVSTVGGAMVD
                     PAFNNYLRDTFHLAADKRGQLELPRELPGFLVTVTSGLLFFLPEVRTAAVATAATAIG
                     IGGLAWAGANYGVMIGFMVLWSTGLHLMMPMRSSITLAFAKEGRQATLLGTTGSITTV
                     AAILGSAIGWWIFGTLGTNPRTYNAIFILGASLTGLSALILLKMRVPATAQDQPRPKL
                     VVKRRFIVYYLMSMINGARKQVFITFAPWVLITIFHRGPSTFAALAITSALLGIVVQP
                     TLGGLIDRLGERRILMMEGFFLFFVCLGYGFAHRISTGGTVILIVYACFVLDNLLFSV
                     GMARVTYVNKLAEKAEDVTATLSLGVTLDHVISMSIPILGGATWVIWGFEYVFAGAAG
                     LAVLSVFVASFVRVPRVPLRTPIVVTEEEGPGTCRTEEIGRTSA"
     gene            8045..8767
                     /locus_tag="P5179_04795"
     CDS             8045..8767
                     /locus_tag="P5179_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRS94572.1"
                     /translation="MSNRRDWPHVRLMMATTLDGKIATSRREAARFGSPEDARRLETQ
                     VAWADVLLFGAATLRAYGTTYRVRREALLRLRQRRRKPPQPVTAVVTSSLDLPLEMPF
                     FTRQDVPRVIITAESEKDRATVQFCGLAEVIARGTSAVDWNAVLAEFKTRGLKRVLLL
                     GGGHLNAELLSLGCVDEVCVTVSPFVFGGADAPTIADGNLAAKHWRSEPQLRTWELVG
                     EEVFCTYRFRKRSPVNGSVRTQ"
     gene            complement(8852..11359)
                     /locus_tag="P5179_04800"
     CDS             complement(8852..11359)
                     /locus_tag="P5179_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94573.1"
                     /translation="MQIAVFVGFLLVSGASIRVEAQTWSATHPGYPCTGARALGMGTA
                     IVSSTDGPTALAWNPAGLVEQVFPGIAGDLGSTSLRNSALFFGSPVSQDGAIGLGWVR
                     NRRILPYPGTPEGLDRFAAGVGIRLTDKLAVGMSGSFLSPQYRAAPDSAREAAGFSTD
                     IGALFSLTGRICVGASIYDLTDAKVWREDGRVETLNPRSVRWGVSFDASDQVTFAYQR
                     DQGDRIGVEWFPVESMALRGGVGYRDRMWSRSGGVGFRSGSVRVDYAYAQLEQSYHQH
                     RVSVAMVFPRGRSAVQITKPQVRSLYASLGKAYTREPVASIELVNSSSNPVTTRVELL
                     LPRFMTAPSSEEVTLRPYSRQRIFLRAVLSGEQIAAVADQPVQGVISLVNPATGEVWR
                     RHTVDTFLYRRGALTWDDVRKASAFVTPADPDVRAFVRESLRPYRSRLQQLPLTQEII
                     TTAMVLFEAAGEVGITYQSDPNTPFSEVRKTENPLDAMRRIGANTLTLAVKPVEIAAN
                     PIRNVISGPDSARGDTLGVIGNAVSGVSGMITGVGEIGEESLRLLLFPYQALAGDLNQ
                     NPVVDDILYPAELLRVRAGDCDDTTVLLCSLLECAGLTTAFVEAPDHIFMMVDTRLPV
                     DESPYVLPAPVERFVAFEGTWWLPLETTLFPQGFMAAWSNAAGVARSLASQGNLRVTR
                     VAEGWALFEPIEFHASPDEEIRFPDSRNLERRVTLQDSALAVLWKDSPVARDLLASVG
                     ASTGGSEEDKRRADAAFWLLFAGRLSDADQHLRVLEEHAYDPPRVMGLRAVYWFFSGD
                     VIRAEEFADQAYQAAPGIPDLLDLRTQIRSARSGVSE"
     gene            complement(11372..>11628)
                     /locus_tag="P5179_04805"
     CDS             complement(11372..>11628)
                     /locus_tag="P5179_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004598174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRS94574.1"
                     /translation="FEDLGPDEAINRCVDAGVDTIAPRASLIDVEFVRKAHAAGLLVR
                     AWGVADDTGPGLRRLIAAGVDGATTNHPNVLKEILNEPLS"
BASE COUNT         2424 a         3477 c         3407 g         2320 t
ORIGIN      
        1 cgcgttcctt ctcggcgcgc gcgctccttt ttcctgtagc catagttcct ctcatcctgc
       61 ctccgcctga ttctggcgcc ggaacggagt gctctagatg ccaaccagcc aactgaaaag
      121 ccagttcatg agcgagatta ccacactccc ttttaacgcc gcgatgaagc cgtccaccac
      181 gagcataggg acgaaaaccg gggtcatcca aaggatgagc gcgttgatca cgaagtagaa
      241 aagccccatg gtgagaatca gcacaggaag ggaaaagaac gtgaggattg gcttcacgac
      301 ggcgttcaac aggccgaaga cggcacccac aagaagcagg tgccaccagg cccatggctc
      361 caccagggcg acgccgtcca ccgcacgggc agcgatgtag atggaaagac acgtaagcaa
      421 aagcctgagt atcagatatc gcacggatac ccccgatcgg aaatacggtt ggccctgttc
      481 tctctcttcg gtccgtctac cggtttcaga gtgattacca aatcgtcgcc tgtcaaaagc
      541 tggtttttgc aaacgctgtt acccctttct cgcgcccatc gcggtccccg gccggtgtgg
      601 agaatggcta cgccacacga tcggggaaga gtgttttgat gttgacgacg gcacgttctg
      661 ccaccgtttc aaggcagcca agggcaagtt tcgcccagta cgtgacttcg ttgttcgctt
      721 cgtgtccccc gcgcgcgtag gcttcccgcg tgggcaggta cccgacgtag tgagacacca
      781 tgtgggtggg aaacgtaaac ggtacgggcg aagaagtttt gatcgccaac tgaccctcga
      841 caaacggttc gcccggcaat cccacgatag caaggtcgcc tacgcggaac acctgcatct
      901 cgtaacgcat atgcgtttca cgttgtttga gtatctccac gctgcgtgtg gacgcggcag
      961 tgaaccagac aggatccact tctccgtttg cacttcgcgg cggctccggg ctcctctcga
     1021 gtatttcggc aaccatgcgg cggcgttcgc cgggaatttc acggaatgga aggctgacgc
     1081 aatccgcgca actcgctacc tgcgcacctt cggcaaattg catcgtgtgt acgagcttct
     1141 cgcttacttc cgccagggcc ttgcccatgc gacggtggtc tggacgcgcg tccggttcaa
     1201 acgggttcca tggattgatg ttgccgcaac agccattgag aaccagtgga acgacgtgcg
     1261 aaccgtacgc acgcttcatc ccttcggccc atgctcccgg ccaatcggag gagaccgccc
     1321 ggtacgttcc tctggagccg aacacgttca ccggatggca cgtatggtgc aaaaggaacg
     1381 cgatcgggcg catatccaga ccctggaaac acgcaacgcc cacttcggga tccatcggac
     1441 cttccatgta acatagctct gtgatcccga agggttggtt ttcgcgcccg gcaggctttg
     1501 gcattatgat gcggccgtcg cgcgttacgc cccggcgatt gaacgagaac tccgcattca
     1561 gggcacgggc caggccgact ctcgcgggct gaagccggtc gtgtgcttcg atggccgctt
     1621 gaacagcttt tttcgaagcg aactcgcaat acggagtttc agagccccag aggtactcgg
     1681 tttcgggggt ggtttccagc gggaaatccg ggtccagcat gaaacgcccg acgctgggcg
     1741 cggaatgagt ctgttcggcg aacaccatga tcgcttcaat gggtatccct gtggcgtcgc
     1801 tcacgtcatt gcgaatccgg gtggagtatt cacccgtgat gatcgtaacg tcgagggaga
     1861 tcacgcagac tcgcgcacgc ccgtcgtcga acacaattgc ccgtgcgtac gtcctgtcca
     1921 gcaccgactg agccggtctg cgatcgccgc acgcgctccc cgagagatat gctccgtacg
     1981 gcggggtaat gtcctgctcc gacgctccca cacggaaagc gaaacgcgag gtctgccgaa
     2041 cacgagcggt catgccgttt gctccaaggg tttcatagtt cctcgccagt agtcgtcatg
     2101 acgaggcctc catgtttgac accacgggac cctatgagga gcccggcaag ctccttcacg
     2161 tcgtgcgcgg aaccccgggt gacaatcact tcgaggcagt tatcgtggtt caggtgaacg
     2221 tgaaggctgg agatgattcg atctgtgcgt tggtgctgga cctctgtcaa acgttccgcc
     2281 agatcacgca catggtggtc gtacacaaga gttatcgtgg cgacgacttc ctcgcctgca
     2341 gcccactgct cggccacaag gcggtctcga atcaggtcac gcagagcctc tgagcggttc
     2401 gagtaaccgg actccctgat tagcctgtcg aaacgcttga ggaggtcctc tcccaccgcg
     2461 acgctgaaac gcgcaagtcc tcccatgcgt gctcctgatt cagcgaacga gtatgtcggc
     2521 gcggcggagc gacccccata accccgcagg cacctcgacg gaaggccaat ccctgatcat
     2581 ggacaggatt tccggcccat ccggagtcgc tatcatcgtg tcttccgact ttgtccctgt
     2641 gatggaggga ttccatgcga acgcctgcca ggcctgcacg cgctccttgc agtggagaga
     2701 gcccttgaaa tcgcggctcg cgtagcccgt tccgccgcct tgatggtgca acatccactc
     2761 gtctttgaac cctgtttcct cgtagacgtc gagccctttc tggaaaacct cgtcgacgtc
     2821 cgcgccgatc agggtagcgc cgatataggc cgcgtctacc cgcatcaccg cgttgtgctt
     2881 cctcttcagt tccgccggta gtttcccgaa atggacaagt ctcgttgttg agaggatgag
     2941 accgtgtctt cgaccacaca tgaccaccat cacatatttc gcgattttgt tcgccgtggg
     3001 tatcggatgg cggtatttcg agattcggtc atctgccgcc acaagcacca ctgcagggac
     3061 tatctcacgc tcccacagcc gtttggaaag ggcggccgct acctcccact ctgtctggcc
     3121 cggttcaacg gcaaggcaga gatcactcat cacctcagcg cagtccttgc cgagggcgcg
     3181 gtagcgctcc acttccaccg gtgtgaggct gtaacgaagg tggttcaatt cgcccgcgaa
     3241 ttgccccgtg tccgtcgcat atcgctggcc cgcaagcact cgctcgacga ggcgggtggc
     3301 atcggtctca ggactatccc actgatagac aagtggttgt atgccaagct cgctcaaccc
     3361 ctcttcatcc atgatcctgg gcgcttctat gttgttcgca acgaggtaat cctggtctgg
     3421 agtcgccagc aggtgagcag cgccagcttc agtggtcaat cccacatggt tttgcccacc
     3481 gcttgtgtac cagctgaaag acttctgctg cgtaaacaag accccgccaa gatcatgact
     3541 gcggaggaag tccctcacgc gcactctttt cagcgcaatg tcgtccgctc gcgtcattgt
     3601 cgtgtcgtcc ttgtcgaaag ggagcaacaa tcaaaaaacc ctgtcaaaat atcgcacaag
     3661 cgatgcacta ataccatgct ctcgaccgct cccctggttc gacaggggta tgccgatgga
     3721 agtggcgttc cacgcgctgt tacggtctgg tcagggggga ttatttgacg tgagagtgcg
     3781 aggaagcgcg gaatgacggc atatcggcaa atggtatttt gccgaacaga tttcaaaggc
     3841 ttgagaggaa cccgtccgcc aggcgggcgc cgaaaatctg ggggatcccg gatcggttgg
     3901 aggtgaagat cacccaacgg ttgtcggcgg tgaaatacgg gtgcgtatga gaccattgtt
     3961 ggccgtcgtg aacggttcgg ctgaaacaga gcctgctgtt cgaacctgtg acaaaggaac
     4021 cgatgtagat cgggatacct tcctcagacg gagcgtccgc gatccagaac cggccgcaac
     4081 ctgatgtcgc gacgtgcccg aacacatgct ctcccacaca tgcggctgcg ggttgttcgt
     4141 cagaaagaga aacgctccac acgtttgtca ttgaagacga atcatacgcc gtcgaaagga
     4201 acacgcgatc tgtttttcca atccaggctt catgccccgt acacctcggc gtgtaacgat
     4261 ccctgcccgg ccatttcccc actggaacca ggggacggga atccaccggc agccattcaa
     4321 tctcgccgcc gcagggatac accacgccaa gattcacaac ggagacatct gcgctgtttg
     4381 cctgaatgag aattgcgtcc cggccgtccc gtgaaaactg ctcgtgcttg aaccattgcg
     4441 cgctgctttc ggcaagaacg gaatgcaccc catctacgag gtcaaaaagg aggagtcgcg
     4501 aggacgtgct gtcaggatgt gagttgaccg cgtaatactc cacatcaggc gatacagttc
     4561 cgtaactgag ttggctccca gtcgcgggaa gcaccaacac gtcttccatc gtcgtgttga
     4621 cgtaatgaca ccgccgcaga agcatttcgt ccccaacgta ttcctggaaa tacaggtatt
     4681 cgccccacgc gtggaacgcc gggaaacggg gcatcttttt gaggacgggg tgcaacgacc
     4741 cgtccacgag atcaaggatg gataacccgc cgtctgttcc ttcgtgcggg taataccgga
     4801 ccgcgatacg agttccgcag ggagaggaat agggctgttc accgtagatg ttgtccgcgg
     4861 ggcggggacc atccgtgagc tggtagatct ccgcaccgga cacagtgtca cattccacga
     4921 agtgcatcag caagtaccct cagacttcgt cgcgcgtcgg gagaggtaag cgcgcgggct
     4981 caggaatcca gaagaagatc caattccatg tcctgactga agatgcccgg aggtccttcg
     5041 aacccgccta cccgccggaa accttgtttt tcatagaaat ggatgccgcg tgtgttcagg
     5101 acttgaactc cacccatcag gaccatcttc cgccggccca gtctccgggc cacgtgaatg
     5161 acgtgcctga aaagcggggt gcccagcccc tgattctgga actgctcgcg aactgacgga
     5221 gcaaatgtgc agtcctgggc cgggtccagc tgaattccca catcagcata gcgcttgccg
     5281 tccgattcac ttgttccgag gacgaggata aaatacgcaa ctatctgctc cctgccatca
     5341 acgcgtgtca aggcgacgaa acggatggtg tcgtcgtaat tgatggatcc gcagagctga
     5401 tctgccgttg cctgatcgaa tgcatgaggt ccgtaaaaac gagtagtctc gcgggtcaga
     5461 ctggtgaaat actcgccaag caaggtcgca tcttcggcgc gcagcggtcg aaacacaacg
     5521 tgttccccgg aaggcagacg cagctccatc gccgcacaca ttgggtctgc cgtgatttcg
     5581 gcggtacgta gaggttgcac agacatgggt ttcgcgtatc acctttcgtg aaggattctg
     5641 aggacggctt gaagccgcag tgaatggatc gtttccaatg tagccagcgc cgggcaggca
     5701 acgcaatgag gcaagagggg agggggagag agctaaagaa ccttcacggc aggccacttg
     5761 gtggcccggc aacgcaggcg gtacttttca agacacccgt gtcccggccg atctgcacgt
     5821 gacctgcgat ggctgccccg caacgttccc ggaatggagt ggagaaccca tggcgtcgac
     5881 gattcgcgtg gcgatgattc agcgagcatt tgttccgttt gagcgcgaag caaacatttc
     5941 aatcgcggaa cgtgcgattc gtgcggcggc gcgagacgga gcccacctgg ttgcgttgcc
     6001 cgagatgttt ctcacgggat accctcacgc acctgcgaag gattccgcgg attggccgca
     6061 attcgttgag cgcctctacg gcacggcgga aacggcagat ggaaccagcg cacggaaact
     6121 gggagaaatc gcgaaagatg ccggcgtcgt tctcatagcg ggcatctgcg aaatccgcga
     6181 cggaaggctg ttcaacagtg cgttcgtctg ggattcaaag ggagagcaca tcgggactta
     6241 ctgcaaggtg catacgtgca ggttcaccgc gttggaagac ctgtgcgaag acggggacga
     6301 ctggcacgtc tggccgctta cggtcgggga ccaactggtt cgcgtcgggg tgatgatctg
     6361 ttatgaccgg gaacacccgg agagcgctcg tgttctcatg ctgaagggtg ccgaccttat
     6421 catcgttccg aacgcctgcg ggattgacga aagacggctc attcagctcc gcacgagagc
     6481 gtacgagaat gttgtgtaca ttgggatggc caaccacagc aagccccaca atggacagag
     6541 tgtcatcacg aattactggg gagacatcct cacgcaagcc ggcgaaggtg acgagaccat
     6601 cttcgcggac ctcgatcttg aagcgctccg agacgagcgg acgcatacca tatggggcaa
     6661 caatttccga cgtccctgga aatacgggct gatcgcgaag acttagctca ccctgtgccc
     6721 cggggcgcgg caactgctcc cgctcccaca ctccagactg gaaccacgat ttgaagagct
     6781 ttgtatcccg tgcacatggc gtggcaacca acagcctccg acgctggaac gcgcagtcgg
     6841 tggatttccg ctggttcatt ctggcgcttt gtgtgtcgac agtcggcgga gcgatggttg
     6901 accccgcctt caacaactac ctgcgcgaca cgttccacct cgccgccgac aaacgcggcc
     6961 aacttgagtt accccgcgaa cttccaggat tcctggtaac cgtgacttcc gggttgctct
     7021 tcttccttcc cgaagtcaga acggcggcgg ttgccaccgc ggccaccgct attggaatcg
     7081 ggggactcgc gtgggcgggt gcaaattacg gggtcatgat cggtttcatg gtgctttggt
     7141 ccacaggcct tcacctgatg atgccgatgc ggtcttcgat cacactggcg ttcgcaaaag
     7201 aaggccgtca ggcaactctg ctcgggacta cgggaagcat caccaccgtc gcggcaattc
     7261 tggggtcggc gatcggctgg tggattttcg gcacgctcgg cacgaacccc cgcacataca
     7321 acgcgatatt catcctcgga gcgtcgctga ccgggctgag cgcactgatc ctcctcaaaa
     7381 tgcgcgtgcc tgcaaccgcg caggaccagc caaggccgaa gctggtagtg aagcgacgct
     7441 tcattgtgta ctacctcatg tcgatgatca acggtgcacg gaagcaggta ttcattacgt
     7501 tcgctccgtg ggtgcttatc acgatttttc accggggacc ctccacgttt gccgcccttg
     7561 ccatcacatc tgcgctcctc ggcatcgttg ttcagcccac cctgggagga ttgattgatc
     7621 ggctgggaga acggcgcatt ttgatgatgg aggggttctt cctgttcttc gtctgcctcg
     7681 ggtacggatt tgcgcaccgg atcagtacag ggggaacggt cattttgatt gtttatgcgt
     7741 gctttgtgct ggacaacctc cttttctccg tcggaatggc gcgggtgacg tacgtgaaca
     7801 aactggctga gaaggcggag gacgttacgg caacattatc cctgggtgtt actcttgatc
     7861 acgtgatatc catgtccatt ccgattctcg gcggagcaac ctgggtaata tggggtttcg
     7921 agtacgtgtt tgccggcgcc gcaggtctgg cagttctcag cgtcttcgtc gcgtcgtttg
     7981 tgcgcgtacc cagagtcccc ttgcgcacgc cgatagtggt tactgaggaa gaaggcccgg
     8041 gcacgtgtcg aacagaagag attggccgca cgtccgcctg atgatggcga cgacgttgga
     8101 cgggaaaatc gccacctcac gaagggaggc agcgcgattc ggcagccccg aagacgcccg
     8161 ccggctcgag acacaggtgg cgtgggccga tgtcctgctg ttcggcgcgg cgacgttgcg
     8221 agcctacgga actacctacc gggtgagacg ggaggccctg cttcgcctcc gacagcgccg
     8281 cagaaagccc ccgcaacccg ttactgcggt agtcacatcc tccctcgact tgccgctgga
     8341 aatgccgttt ttcacccggc aggatgttcc ccgagtaatc atcaccgccg aatccgaaaa
     8401 ggaccgcgca acggtgcagt tttgtggtct cgcggaggtt atcgccagag ggacgtccgc
     8461 ggttgactgg aatgccgttc tcgcggaatt caaaacacga ggactgaagc gagtactgct
     8521 tcttggtggg ggccatctga acgcggagct gctctccctc ggctgcgttg acgaagtgtg
     8581 cgtaacggtc tcaccttttg tgttcggtgg cgctgacgcc ccgactattg cggacgggaa
     8641 cctcgcggcg aaacactggc gaagcgagcc acagctgaga acgtgggaac tggtggggga
     8701 agaagttttc tgcacgtacc gttttcggaa aagaagcccc gtaaatggtt ctgtaagaac
     8761 acagtgagga gacggtatcg ggggcgagga acctcccgat tcggttactt ctgagcaagg
     8821 atgcgtcagg tggagtgaag aaccccggac gtcattccga cacaccagaa cgcgccgagc
     8881 gaatttgagt acgcagatcc agaaggtccg ggattccggg cgccgcctgg tacgcctggt
     8941 ctgcgaattc ctcggcccgg ataacatccc ctgaaaagaa ccagtacact gcccggagtc
     9001 ccatgactcg gggagggtca tacgcgtgct cctcaagcac gcgcaggtgc tgatccgcat
     9061 ccgaaaggcg accggcgaac aggagccaga acgcggcgtc ggcacggcgt ttgtcctcct
     9121 cgctgccgcc ggtggacgct cctaccgacg ccaacaggtc gcgggcaacc ggggaatcct
     9181 tccagagcac agccaatgca ctgtcctgaa gagttactcg ccgttccaga ttccgggagt
     9241 caggaaatcg gatctcctcg tccggggaag cgtggaactc aatgggttcg aacagcgccc
     9301 aaccctccgc gacacgggtg actcgaaggt tgccctgcga cgccagcgaa cgagcgacgc
     9361 ccgcggcatt gctccacgcc gccatgaatc cctgcgggaa caacgtcgtc tcaaggggaa
     9421 gccaccacgt gccctcaaac gcgacgaacc gctccacggg agccggtaac acgtaggggg
     9481 attcgtcaac ggggaggcgg gtgtcgacca tcataaagat gtgatccggc gcttcgacaa
     9541 acgcggttgt gagccctgcg cactcgagga gcgaacagag aaggacggtg gtgtcgtcac
     9601 agtctcctgc ccgcaccctg agaagctccg cgggatagag gatatcgtcc acaaccgggt
     9661 tctggttgag atctcccgcc aacgcttgat acggaaagag cagcagtcgg agcgactcct
     9721 cgccgatctc ccccacaccc gtgatcattc cggagacacc ggaaaccgca ttcccaatga
     9781 ctccgagagt gtctccgcga gcagaatcgg ggccgctgat cacattcctg atcgggttcg
     9841 cggcgatctc aacgggtttc acggcgaggg taagcgtgtt tgcaccgatc cgcctcatcg
     9901 cgtccagggg attctcagtt tttcgtacct ccgagaaagg tgtattggga tcgctctggt
     9961 acgtgatccc gacctcgccg gcggcttcaa acaacaccat agctgtcgtg atgatttctt
    10021 gagtgagtgg gagctgttgc agacgcgacc ggtagggccg gagtgattcg cgcacgaacg
    10081 cccggacatc cgggtctgcc ggtgtcacaa acgcggaggc cttcctcacg tcatcccatg
    10141 tcagcgcgcc gcggcgatac aggaacgtgt ccaccgtgtg tctgcgccag acttctccgg
    10201 tggccggatt gacgaggctg atgaccccct gaacgggttg atcggcaacc gccgcgattt
    10261 gttcaccgga gagaacagcg cgcagaaata tcctctgacg ggagtacggg cgcagtgtca
    10321 cttcctcgga agatggtgca gtcatgaaac gcggaagcag gagttccacg cgtgtagtga
    10381 cgggattgga agaggagttc accagttcga tggaagccac tggttcccgc gtgtaggctt
    10441 tgccgaggga ggcgtagaga gacctcacct gtggctttgt aatctgcacc gcggagcgtc
    10501 ctctgggaaa aaccattgca acggaaactc tgtgctggtg gtatgattgc tccagttgcg
    10561 cgtacgcgta gtcgacgcga acactgcctg agcgaaagcc gactccccca cttcggctcc
    10621 acatccggtc acggtacccg acacctcccc tcaacgccat ggattccacg ggaaaccatt
    10681 caactccaat gcggtcgccc tggtcgcgct ggtatgcgaa cgtcacctga tccgacgcgt
    10741 caaaggagac gccccagcgg acgctgcgcg ggttcagggt ctcaacacga ccatcttcgc
    10801 gccacacctt cgcgtccgtc agatcgtaga tcgacgcccc tacgcaaatt cgccccgtca
    10861 acgaaaacag cgcaccaata tccgtactga acccggcagc ctctcgcgcg gaatccggcg
    10921 cagcgcggta ctgcgggctc aaaaagcttc ccgacattcc gaccgcgagc ttgtcggtca
    10981 gacgaattcc tacccccgct gcaaaccgat ccaggccctc cggggttcct ggatacggca
    11041 ggatacgcct gttgcgaacc caccccagcc ctatcgcacc gtcctgtgag accgggcttc
    11101 cgaagaacag cgcgctgttt cgaaggctcg tgctcccgag gtcacccgca attccgggaa
    11161 aaacctgctc gaccaggccc gccgggttcc acgcgagggc cgtggggcca tccgttgagg
    11221 aaacgatcgc agtgcccatg ccaagcgccc gtgcgcccgt acaggggtac ccgggatggg
    11281 ttgcgctcca cgtctgggct tccaccctga tcgaagcccc agacacgaga agaaagccaa
    11341 cgaacactgc gatttgcatc gccagcaccg ttcacgaaag gggttcgttc agtatttctt
    11401 tgaggacgtt gggatggttt gtggtggcac cgtccacccc tgctgctatc aacctccgca
    11461 gtccgggtcc ggtatcgtcg gcgacacccc atgcacgcac aagtaatccc gctgcgtggg
    11521 ctttgcgcac gaactcgaca tctatcaggg acgcacgagg cgctatcgtg tcgacgcccg
    11581 catccacgca gcgattgatt gcctcgtcgg gaccgagatc ctcgaagg
//
LOCUS       DAOTNG010000067        11561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5289_length_11561_cov_5.265861, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000067 DAOTNG010000000
VERSION     DAOTNG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_11561_cov_5.265861"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="P5179_04810"
     CDS             complement(<1..1052)
                     /locus_tag="P5179_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HRS94575.1"
                     /translation="MRWVTLLFSAGLLFGAWVPGGASEHCCSGALDRLHGAGYENLAC
                     ETTDSSVVVWCENRRIRYPVVWMIEALTVVASSVPDDVVVNVVAERLAVPVTSLSARA
                     GDVRAWVKGRIGTEEFRQLLRVEFDGSECPSERRNSSLRKVDLAIGPGRILSEFGLPG
                     VWIRANFDVSTEFSTAFLPGLNASGRMFFPAYNTGGPVYNDSRYNELRPGSALLSYLR
                     PVGKSGFLSVCGGMVEMANWRYDSYGIVVDAKQYSPDGTWGFGGSFGYIAPGRYQVLN
                     DANQRYRTWVFVWPLYNTPYEAWTSYRVTGFDLRITARWGRYLVGDRAWRIDVERSFG
                     ETKLTVFGIQSNGYSD"
     gene            complement(1080..2258)
                     /locus_tag="P5179_04815"
     CDS             complement(1080..2258)
                     /locus_tag="P5179_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94576.1"
                     /translation="MRRGLLVLVAGIAVCAAVCASPVHAVNRYPVSIGISYGSWTPSM
                     DAYNVRWVDQSNPTMINVGGTLTPVYIRQPLDSAQTIWGKRVGPESFLFSSSSGLGLN
                     AKLRLHNDVFALVEYDSWKQSVGSRRNYGGMIGYEANEVKLNPISVSLLYYIPTEAGK
                     WWPHIYLGAGGGSVLVERSNSQLTNTGILSTVTSTGSGPLFVGMAGLEYTVPALQDRL
                     TLFFQGRYTMGSFDDQFPVLGSTGSQLIDPVTGKPRKEDASVSIQGSQMKFGLSVNFG
                     QLVSRPTKGVLSGFLEANRRRGGYAMAPSRGMQSGGYAMMVPYSSESVQVVEGAGQVD
                     EDRIRQVIREELLGARIGVTSARPVDDLAEQQLRSIRERRLQAEQELQQLQELLREEG
                     "
     gene            complement(2602..3735)
                     /locus_tag="P5179_04820"
     CDS             complement(2602..3735)
                     /locus_tag="P5179_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94577.1"
                     /translation="MVRDLARVGMDSRERLLTAINNGKPDRLPVQVHRWMPYYLRTYL
                     GGIDQWAAYEQFGMDMVIYSDPVVSVDDTEQSRWIRERVLCETDREGVRHWKIRVRTP
                     GGDLTGAEAGNDITTWTTEYLIKTERDFELFEKYCPSPTFDWSPVMRDLNRLGNRGIV
                     RTASRGYGQGAPWQDLCTLMGTVSAIMTALDEPERTRHMLDVILAKRVSTIEKSPPNP
                     SDIVSTGGGAGSDTVISPDLHARFCLPYDRAYHAALREKSPRTKIVYHLCGGVMNLLD
                     LVATNGADGLETMTPPSMGGNADLSAARRMIGDRLFFVGGFDQQQGFERGTPEDARRL
                     VVQCHAACPDGGYICCPSDHFFHGNPENIRAFVDAARECRYDG"
     gene            complement(3742..9159)
                     /gene="uvrA"
                     /locus_tag="P5179_04825"
     CDS             complement(3742..9159)
                     /gene="uvrA"
                     /locus_tag="P5179_04825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS94578.1"
                     /translation="MDVDRNRWLRHLYHGVLHHFGDCGLDTPWKDIPELAKEKLLYGL
                     DGQRIRFRYARNPGERGWTHLDTFEGIIPMLERKFRESKDEGVKEELGAFTSAVNCPD
                     CGGMRLRPEARAVKLGGLSLPELCAMPIEEAHGFLATLPLSSTERQIGQDALNEILAR
                     LEFLMNVGLRYLSIDRTAPTLSGGEAQRIRLASQIGSGLVGVLYVLDEPSIGLHHRDN
                     HRLLNALGRLRDLGNTVIVVEHDEDTMRAADIVVDFGPGPGVRGGAIVGIGSAEDLTD
                     IPDSITGDYLAGRRRIEIPETRRRPNGNWLQITGARHNNLKNVDVRIPLGVFVCVTGV
                     SGSGKSSLITDTLLPALMRHFFHARLPVGPHDAITGIEHLDKVIHIDQSPIGRTPRSN
                     PATYTGALDPIRRLFAELPEAKVRGYTPGRFSFNVKSGRCDACEGNGATRVQMDFLAD
                     VWVPCPACNGARFNAETLQIRFKGKNIADVLDMDVQDALRFFENIPSVHRVLQTLHDV
                     GLDYMKLGQPSPTLSGGEAQRIKLARELCRRSTGRTLYILDEPTTGLHFHDVSHLLEV
                     LHRFCDEGNTVVVIEHNMDVVKTADWILDLGPEGGDEGGRLIGTGTPEEVAEIDGSWT
                     GQVLRDVLHGKVYRRATSRVSEDDGHSRAAIAFDRDEYRPMERITDITVVGAREHNLK
                     NVSLAVPREKLVVFSGVSGSGKTSMALDTIYAEGQRRYVESLSAYARQFLGDIQKPKV
                     DQVEGLSPAIAIEQKGAGRNPRSTVGTITEIYDYLRVIYAQIGEVYCPDCQIPAGAQS
                     SSEVVERLTEEFRGRRIMLLAPLETEKGGDYDDALARLRKEGYLRCRIDGVVHRLDEG
                     IELDRRRNHSLEAVIDRLEISDDERGRLGEAVETAFNKGEGRLVVAPLDGGDDIAISR
                     HISCPSCGKSFEKLTPQMFAFNRAHDISASGMCPACQGLGTQQGLAEAAVIRSEKLSI
                     AEGAVDLWGKPKGMFRALLEAAGNALGFDLHTPLREMTQAARTALFFGDESRWISCTE
                     RGIEFQYLGVFPAVDRTLRLTPQLRDRLGQVLSDVPCSTCGGTRLIPQSRAARLRGVP
                     IAELCSWPLAQTRAFFDQLSLSPREQQVVGEVLTEIRSRLRFLEEVGLGYISLDRRAP
                     TLSGGEAQRIRLASQIGSGLTGVLYVLDEPTIGLHPRDNALLLSALEHLRDLNNTVIV
                     VEHDSDTLNKADYVVDFGPGAGNSGGEVVATGTPGEIRQNQASLTGAFLSGRRTIPVP
                     RQRRAGTGKAIEIIGAREHNLKDIHAVFPLGTMTVVTGVSGSGKSTLVNDTLHRALAC
                     ALHKAQFVPGAHDEVRGTSFVDKVILIDQEPIGSSSLSNPATYSGVFDVLRELFAQVP
                     EAKMRGYTARRFSTNVPGGRCERCWGYGKRHIEMHFLPDVWVECEDCHGLRYNRETLE
                     ITFGGVSIGEVLQMPVEVALEHFQRVPRVRRLLQTLCDVGLGYMELGQSATTLSGGEA
                     QRLKLASELARPSTGRTVYILDEPTTGLHFADTERLLDVLNRLVDQGNTVIMIEHNVL
                     VIQAADWIIDLGPEGGDAGGYIVVAGTPEDVAACERSHTGAVLRRALAQKVPAVSNRK
                     DAREAIAVVETETQGLEEFPEEDVPSGGPAPPWKTDGIAWHTSDNRLREGRQPLWKGA
                     LIFWIIGQIKKIAPVSVVWSNQHLVSVYPERARRWIAFRTDSWQFVTMEVRVPKGSFD
                     GDKLRKQLDLKPFDELDDVPLYGSWSRVTMNGRIRTWTMVEIVAFRQEELDTEGFRSF
                     LRDAYQLFMDHEAKGGKARSG"
     assembly_gap    9169..9268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9269..9963)
                     /locus_tag="P5179_04830"
     CDS             complement(<9269..9963)
                     /locus_tag="P5179_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRS94579.1"
                     /translation="MSADQIVIRGARVHNLRSVDLTLPKNRLICFTGVSGSGKSSMAF
                     DTIYAEGQRRYIESLSAYARQFMEQMEKPDVDQISGLPPAISIEQKAAGGNPRSTVGT
                     MTEVYDYLRVLYARLGTLHCVQCGKPITGQSRDRLLDQILQHPPGTRLNILAPMARGR
                     QGEFLDLFEELQRQGFVRARVDGRIVNLSEDPSLDRHAKHNVDVVVDRLVMREGIRSR
                     LAEAVDTALNVGNG"
     gene            10441..>11561
                     /locus_tag="P5179_04835"
     CDS             10441..>11561
                     /locus_tag="P5179_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94580.1"
                     /translation="MSADRRLRVLMPVLLCLVSAAPILLAPGTSRAAPGSVTNLTATA
                     TGTNEITLSWTPYSGNDFARYEIRRGTAAGVTTASPLVDTLSAKTASSFADRTVLPYT
                     TYYYKVFVIDTAGVAGSSAEPSARTLALSYPFADTVAAANVNFVPTGTWAIVANSPGE
                     GDAYSGSWHWSDSPGGNYAPNSNGTLTLAIRLGTATMPVLTYRERYAFETNVDYGHVE
                     VSADGTNWYAVASVTGSQTTWAERRIDLTQWAGYDGIRIRFRIAASSSVESDGWHLDS
                     FSINETPLASMPYPLREDFESGTSRWLLGGWSPATEGTNGPTSIHDSKTGNYPALAYN
                     GMVSTGVFDLTFAKNPTLVFWHRYNIFNDHSYGHNEDDWG"
BASE COUNT         2344 a         3721 c         3073 g         2323 t
ORIGIN      
        1 taatcagagt acccgttcga ttggattcca aaaacagtga gcttcgtttc gccgaacgaa
       61 cgctcgacgt caattcgcca cgcacgatca cccacaagat acctgcccca ccgggcggta
      121 atgcgcaaat cgaatccggt taccctgtag ctggtccacg cttcgtacgg cgtattgtaa
      181 agcggccaca cgaaaaccca cgtgcgatag cgctgattgg catcgttcag aacctgatat
      241 cttcctggtg caatgtaacc gaaactgccg ccgaatcccc aggtcccatc tggcgaatac
      301 tgcttcgcat caaccacgat accgtacgaa tcataccgcc agttcgccat ttcgaccatg
      361 ccgccgcaaa cggaaagaaa gccgctcttc cccaccgggc ggagatacga caacaacgcc
      421 gatcccgggc gcaactcatt gtaacggctg tcgttgtaaa caggtccccc ggtgttgtac
      481 gccgggaaga acatacgtcc cgaagcattc aacccgggaa gaaatgccgt cgaaaactcg
      541 gtgcttacgt cgaaattcgc acggatccac acgccgggaa gaccaaactc actgagaatc
      601 ctgcccggcc cgattgcaag gtccactttc ctcagtgaag agttgcgacg ttcggatgga
      661 cattccgacc catcgaactc gacacggagc agttggcgaa attcttccgt tccgatgcgt
      721 cccttcaccc atgcgcggac gtcacccgcg cgggcactga gcgaggtcac aggaaccgcc
      781 agccgctccg ccacgacgtt cactacgacg tcgtccggaa ccgaggatgc gacaacagtt
      841 aacgcctcga tcatccagac gacagggtac cggattcttc gattttcaca ccacacgacg
      901 acactggaat cggtggtttc gcacgcgaga ttttcatacc cggcaccatg cagccggtcc
      961 agcgctccag aacaacagtg ctcggacgcc cctccgggca cccaagcacc gaacaacaat
     1021 ccagccgaga aaagaagggt tacccagcgc aatgcgccgc tccccgtatc ctgaggggat
     1081 cagccttctt cccttaacaa ctcctgaagc tgttggagtt cctgctcggc ctgcaacctt
     1141 ctttcgcgaa tgctgcgaag ctgctgctcg gcgaggtcat ccacagggcg agctgaagtc
     1201 acgccgattc gtgcaccaag cagctcctct ctgatcacct gacgtatgcg gtcctcgtca
     1261 acctgtcccg cgccttctac cacctgcact gattccgaag agtagggaac catcatggca
     1321 taaccgcccg attgcatacc acgggacggc gccatcgcat acccgccgcg gcgacggttc
     1381 gcttccagaa aaccggacag aacgcccttc gtcggcctcg atacaagctg accaaaattc
     1441 accgaaagcc cgaacttcat ctgcgagccc tggatgctca cgctggcgtc ttctttcctg
     1501 ggcttgccgg taaccgggtc tatcaactga gaaccggttg atcccagaac ggggaactgg
     1561 tcgtcaaagc tacccatagt ataacgccct tggaaaaaga gcgttaatcg gtcctggaga
     1621 gcgggaaccg tgtactcgag accggccatt ccgacaaaaa gagggccgga gccggtagaa
     1681 gtaaccgtcg aaaggatacc cgtattcgtc aactgggaat tcgagcgttc cacgagcacc
     1741 gatccgccac ccgcaccgag gtaaatgtgc ggccaccatt tgccggcttc ggtcgggatg
     1801 tagtagagca gagaaacgct gatggggttc agcttcactt cattcgcttc gtaaccgatc
     1861 atgccgccgt aattgcggcg acttcccacg gattgcttcc aggagtcgta ctccacgagc
     1921 gcgaatacgt cattatgcaa cctgagtttc gcgttcagac ccagcccgct cgaggatgag
     1981 aacagaaagc tctcgggtcc tactcgcttc ccccagatgg tctgagcgga atccaacggc
     2041 tgccggatgt acacaggggt gagcgtgcca ccgacattga tcatcgttgg attgctctgg
     2101 tccacccagc ggacgttgta cgcgtccatc gagggtgtcc agcttccgta cgaaatgcct
     2161 atgctcaccg gataccggtt cacggcatga acgggggacg cgcagacggc ggcgcacact
     2221 gctatcccgg cgaccaacac cagcaaacca cgtctcattg gtggttccct ccctcaatca
     2281 ctcccagtaa ttccttccgg gcggaacttt cagcgcgttc caaccccggc ggaatgcatc
     2341 cgttcatgca gagcctgaac aacgaaagac cttcacatcg cgcagaaatg cgggccttcg
     2401 gcccccgcgc gtataacaga tagtctctca cactagataa ggatacctct cctcaatcct
     2461 tctgtcaagc aagcacacaa gttctttttt tgtcaaagat tttacggtgc tgatccgaaa
     2521 aaacacacga gcctccaaca aggacactcc acaagacctc cgaaccctcg acggggcacg
     2581 ccgcgatcgc tctggtctgc gtcaaccgtc atacctgcat tcccgcgccg catcaacgaa
     2641 tgcgcggatg ttttccgggt tgccgtgaaa gaaatgatcg gaagggcagc atatgtatcc
     2701 cccgtcaggg cacgccgcgt gacactgcac caccagacgc cgtgcatcct ccggcgtccc
     2761 ccgctcaaat ccctgctgtt ggtcgaaccc gcccacaaaa aaaagcctgt cgccaatcat
     2821 ccttcgtgcc gcggaaagat ctgcattgcc gcccatactg ggtggggtca ttgtctccag
     2881 accatccgcg ccgttggttg caaccagatc aagcaggttc ataacacccc cgcaaagatg
     2941 atacacgatt ttcgtgcgag gcgacttctc tcgcagtgcg gcgtggtacg cacggtcgta
     3001 cggcagacag aatctcgcgt gcaggtccgg gcttatcaca gtatcggaac cagcaccgcc
     3061 ccctgttgat acgatatccg acggattcgg cggcgacttc tcgatcgtcg agactctctt
     3121 cgccaaaatc acgtcgagca tgtgcctcgt acgttccggt tcgtccagcg ccgtcatgat
     3181 ggcgctcacc gttcccatca acgtgcagag gtcctgccac ggcgctccct ggccgtatcc
     3241 acgcgacgca gtccggacaa tgccgcggtt cccgagacgg ttcagatctc gcatcaccgg
     3301 agaccagtcg aatgtcggcg aggggcagta tttctcaaac agttcaaagt cacgctcggt
     3361 tttgatcagg tattccgtgg tccatgtggt gatatcgttc cctgcttcgg cacccgtcag
     3421 gtctcccccg ggcgtgcgga ccctgatttt ccagtggcgg acaccctccc ggtcagtttc
     3481 gcacaggact ctttcgcgga tccatcgcga ttgttccgtg tcatcaactg aaacgaccgg
     3541 gtccgagtaa atcaccatgt ccatcccgaa ttgctcatag gctgcccact gatcgattcc
     3601 tcccagatac gtgcggaggt agtacggcat ccagcggtga acctgaacag ggaggcgatc
     3661 aggtttcccg ttgttgatcg cggtgaggag acgttccctg ctgtccattc cgacgcgggc
     3721 gaggtcgcga accacatgtc gtcaaccgga cctcgccttc cctccctttg cttcgtggtc
     3781 catgaaaagc tggtacgcat cgcgaagaaa gctgcgaaaa ccttctgtat caagctcctc
     3841 ctgacggaag gcaacgattt ccaccatcgt ccatgttctg attcggccgt tcatcgtaac
     3901 acgcgaccag gaaccgtaca gcggaacgtc atcgagctcg tcaaagggtt tcaggtcaag
     3961 ctgtttccgc aatttgtccc cgtcaaacga gccctttgga acccggactt ccatcgtcac
     4021 aaactgccag gagtctgtgc ggaaggcgat ccagcggcgt gccctctccg gatacacact
     4081 tacgagatgt tgattggacc acacgacgga aacgggggcg atcttcttga tctggccaat
     4141 tatccagaaa atcagcgctc ctttccaaag cggctgtcgg ccttccctga gtcggttgtc
     4201 ggatgtgtgc cacgcaatcc cgtctgtttt ccagggcggt gcagggccgc cggacggaac
     4261 atcctcctca ggaaactcct ccaacccctg tgtttcggtt tcgaccaccg cgattgcttc
     4321 ccgggcgtct ttcctgttcg aaacagccgg caccttctgc gccagagccc tgcgcaaaac
     4381 cgcgcccgtg tggctcctct cgcatgccgc cacatcctcc ggggtcccgg caaccacaat
     4441 gtagccaccc gcgtcgccac cttcgggtcc aagatcgatg atccagtcag ccgcctgaat
     4501 caccagaaca ttgtgctcga tcatgatgac cgtgttcccc tgatcaacga ggcggttcaa
     4561 tacgtcgaga agccgttccg tatccgcgaa gtggagcccc gtagtcggtt cgtcaagaat
     4621 gtacaccgtc ctgccggttg aaggccgtgc aagctcgctg gccagtttca gccgctgcgc
     4681 ttctcctccc gacagggtag tggctgattg ccccagctcc atgtacccga ggcctacatc
     4741 gcacagcgtc tgaagcagac gccgcacccg cggcacacgc tggaaatgtt ccagtgcaac
     4801 ctccaccggc atctgcagca cttcgccaat cgacacgccg ccgaacgtta tctcgagcgt
     4861 ctcccggtta taccgcaatc cgtggcagtc ttcacattcc acccagacgt ccggcaggaa
     4921 gtgcatctca atgtgccgtt ttccgtagcc ccagcaacgt tcacagcgcc cgcccggcac
     4981 attcgtgctg aaccgccgcg ccgtgtatcc tcgcatcttc gcttccggca cctgcgcgaa
     5041 aagttcacgc agtacgtcga aaacgccgct gtacgttgcc ggattgctga gtgaagagga
     5101 gcctatcggt tcctggtcga tcagtatcac tttgtccaca aaactcgtac cgcgcacctc
     5161 gtcatgcgcg cccgggacga attgcgcttt gtggagagca cacgcgagag cccgatggag
     5221 cgtgtcgttc accagcgtgc tcttcccgga accggatacc cccgttacca cggtcatggt
     5281 tcccagcgga aatacggcgt gaatgtcctt gagattgtgc tcccgggcgc cgataatctc
     5341 gatcgccttt cctgtgcctg ccctgcgctg gcgcggaaca ggtattgttc gtcgcccgga
     5401 aaggaatgcc cccgtgagcg acgcctggtt ctgccggatt tctcccggtg taccggtcgc
     5461 gacgacttct cctcccgaat tacccgcgcc gggtccgaag tccacgacgt aatccgcctt
     5521 gttcagggta tcgctgtcat gttccacgac aatgacggtg ttgttcaggt cgcgcaggtg
     5581 ctcaagagca ctcagcagca acgcgttgtc ccgcggatgc aacccgatag taggctcgtc
     5641 aagaacgtac agcacaccgg tcagcccgct tccgatctgc gaagcaagcc gaatgcgctg
     5701 tgcttcccca ccggataatg ttggtgcccg ccggtccagc gaaatgtatc ccaggccaac
     5761 ttcctcaagg aaacgcaggc gagaacgaat ctcggtcagt acttccccta ccacctgctg
     5821 ttcgcgaggg ctgagtgaca gttggtcaaa aaaggcgcgg gtctgtgcaa gcggccacga
     5881 gcacagttct gcaatgggta caccccgaag tcgggcggct ctgctctgcg gaatcagccg
     5941 cgtgcccccg caggtgctgc acggaacatc ggaaagaacc tgtcccaggc gatcacgtag
     6001 ctggggtgta agccgcaacg tgcgatctac tgcgggaaac acgccgaggt actgaaactc
     6061 gatcccgcgt tcggtgcacg agatccatcg gctctcatcc ccgaaaaaga gggctgttcg
     6121 ggcagcctga gtcatttcgc gcagaggtgt gtgaaggtcg aaaccgagcg cgttccccgc
     6181 cgcctccaga agcgcgcgaa acatgccctt cggctttccc cagagatcaa cggcgccctc
     6241 cgcgatactc aatttctccg agcgaatgac cgccgcttcc gccagaccct gctgggttcc
     6301 caatccctgg cacgcaggac acatcccgga cgcagagatg tcgtgcgcgc ggttgaatgc
     6361 gaacatctgc ggagtgagtt tctcgaagct tttcccgcac gaagggcacg atatgtgccg
     6421 gctgatggca atatcatccc cgccgtccaa cggcgcgact acaagcctcc cctcaccctt
     6481 gttgaacgct gtttcaaccg cctcccccag cctgccgcgt tcgtcgtctg aaatctcaag
     6541 cctgtcgatt acggcttcaa gcgagtgatt cctccgcctg tccagttcga tcccttcgtc
     6601 gagacggtga acaaccccgt caatgcggca acgaagatac ccctccttcc tcagacgggc
     6661 gagtgcgtca tcgtaatctc cgcctttctc cgtctccagc ggagcaagga gcattatgcg
     6721 tctgcccctg aattcctccg tcaggcgctc cacaacctcg ctgctcgact gagcaccggc
     6781 ggggatctgg caatcagggc aatacacttc tccgatctga gcatagatca cgcgcaggta
     6841 gtcgtaaatc tcagtaatcg tgccgaccgt gctgcggggg tttcgtcccg ctcctttctg
     6901 ctcgatcgcg atagcaggcg aaagcccttc aacctgatca actttcggtt tctgaatgtc
     6961 gccgagaaac tggcgtgcgt atgcggaaag cgattcaaca taccgcctct gcccctccgc
     7021 gtagatcgtg tcgagtgcca tggatgtctt tccagaacca gacacccccg aaaaaaccac
     7081 cagtttttcc cgtgggacag cgagagacac gttcttcagg ttatgttccc gtgcacccac
     7141 caccgtgata tccgtgatcc gctccatcgg acggtattcg tcccggtcga acgcgatcgc
     7201 cgcgcgcgaa tgaccatcat cctcagatac tcgggaggtt gctcgacggt acaccttgcc
     7261 gtgcaacaca tcccggagga cctgcccggt ccacgaacca tcaatctctg ctacctcttc
     7321 cggcgtcccg gtcccgatca atcgtccacc ctcgtcccct ccctccgggc ccagatcaag
     7381 aatccagtcg gcggtcttca ccacatccat attgtgctcg atgacaacga cagtgtttcc
     7441 ctcgtcacaa aacctgtgca gcacttccag aaggtggctg acatcgtgga aatggaggcc
     7501 ggtcgtcggc tcgtcgagaa tgtagagtgt ccgccccgtg ctcctgcggc atagttcgcg
     7561 tgccagcttg atgcgttgcg cctcgccacc ggagagcgtg ggcgacggct ggccgagctt
     7621 catgtaatca agtccgacgt cgtggagagt ctggagaacc cggtgcacgg aagggatgtt
     7681 ctcaaagaac cggagcgcgt cctgtacgtc catatccagt acgtccgcga tgttcttgcc
     7741 cttgaaacgt atctgcagcg tctccgcgtt gaatcgtgcg ccgttgcatg cggggcacgg
     7801 aacccagacg tccgcgagaa agtccatctg gacgcgagtc gctccgttcc cttcgcacgc
     7861 gtcacaacgt ccgctcttca cgttgaaact gaagcgaccc ggcgtgtacc cgcgcacttt
     7921 cgcttcaggc agctccgcga acagacgcct gatcggatcc agcgccccgg tgtaggtcgc
     7981 ggggttggag cggggcgttc ttccgatagg gctctggtcg atgtggatca ccttgtccag
     8041 atgctcgatc cccgtgatcg catcatgcgg ccccaccggc aaccgcgcgt ggaagaaatg
     8101 cctcatgagc gccggcaaaa gcgtgtcggt tatcagagag ctcttaccgc tgcccgatac
     8161 tccggtcacg cacacaaaca ccccgagggg gattcttaca tcgacgttct tcagattgtt
     8221 gtgccgcgca cccgtgattt ggagccagtt gccgttgggc cggcggcgcg tctccgggat
     8281 ttctatcctc ctgcgccccg cgagatagtc accggttatc gaatcgggaa tgtccgtaag
     8341 gtcctcggcc gacccgattc ccacgatggc tccgccccgg acgccggggc ccggtccgaa
     8401 atccaccacg atatcggcgg cgcgcatcgt gtcttcatcg tgctcgacga cgatcaccgt
     8461 gttcccgaga tctcgcaggc gtccgagggc attgagcagg cggtgattat cccggtggtg
     8521 caaaccgatg gaaggctcgt ccagaacata cagaacaccg acgagaccac ttccgatctg
     8581 gctggcaagc ctgatgcgct gcgcttctcc cccggataac gtgggtgcgg tgcggtcgat
     8641 tgagagatat cgcaacccca cgttcatcag gaactcgagc cgcgcaagaa tctcgttcag
     8701 tgcatcctgt ccgatctggc gctcggttga cgacagcggc agcgtcgcca gaaacccgtg
     8761 cgcttcctcg atcggcatcg cgcacagttc cggcagcgac agcccgccca gtttcacggc
     8821 acgagcctcg gggcgaagcc gcatcccccc gcagtcaggg cagttcaccg cggaagtgaa
     8881 cgcgcccagc tcctctttca ccccttcatc cttcgattcc cggaacttgc gttccagcat
     8941 cgggatgatt ccctcgaacg tgtcgaggtg agtccatccc cgttcgcccg ggttccttgc
     9001 gtaacggaag cgtatgcgtt gcccgtcaag cccgtagaga agtttttcct ttgcgagttc
     9061 cggaatgtct ttccatggcg tgtcaagacc gcaatcaccg aaatggtgca gtaccccgtg
     9121 atacagatgc ctgagccacc ggttgcggtc tacatccacc gtcgccagnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gtttcccacg ttgagcgccg tatcaactgc
     9301 ctccgcaagc ctgctgcgga tcccttccct catgaccagt ctgtcaacga cgacgtcaac
     9361 attgtgcttt gcatgtctgt cgaggctcgg gtcttcgctc agattgacaa tgcgcccgtc
     9421 cacccgcgcg cgcacaaacc cctggcgttg cagctcttcg aacaggtcga gaaactctcc
     9481 ctgtctgcca cgcgccatcg gggcaaggat gttgagacgt gttccggggg gatgttgcag
     9541 aatctgatca agcaggcgat cccggctctg tccggtgatc ggcttcccgc attgaacgca
     9601 gtgcagtgtt ccgaggcgtg cgtacagaac gcggaggtag tcgtacacct ccgtcatcgt
     9661 tcctaccgtg cttcggggat tgcctcccgc tgccttctgt tctatcgaaa tcgcgggagg
     9721 aaggccggag atctgatcta cgtccggctt ctccatctgc tccatgaatt gccgggcgta
     9781 tgcggaaagc gactcgatgt acctgcgctg accttcggcg tagatagtgt caaacgccat
     9841 cgagctcttg ccggaacccg aaacgcccgt gaaacagatc agacggttct tcgggagcgt
     9901 caggtccacc gagcgaaggt tgtgcactcg tgcaccacgg atgactatct gatcagcgct
     9961 catgcgaccc ttgataaccc gaaatgcaga ccaggaagcg gaaagggaca aatactaaaa
    10021 gtactcatac cccggtggca ctgtggtgtc cagtttggaa aacaaccccg atcaacgcac
    10081 gggctgttcc ctccccgtgg cctccgctcg cgaaagaggc gtttctcgcc gcaatttgca
    10141 gtgttcatgc cgcgacgtca cgcagggttg cacgatgacg gtgaagaaat ggatcgcgtt
    10201 ctggcagggc gcgccagaac taccggagag aggattacca ccctgcttct tcgcgtcaaa
    10261 cgggctttcg cgccgggcgt cttgacgcat cactcgaaca gggtattttt gtctgtcgta
    10321 cgaagatgat taacaacatc agtattcttg tgtcaggttc ttagtacggg gcgcgactcc
    10381 gggtgatgtg tatctgaccc tgcgcgaggg cgcctttttg tcgagacaag gggtgacgcc
    10441 atgtccgccg atcgacgcct tcgagtgctc atgccggtcc tgttgtgtct ggtttccgct
    10501 gcgcccatcc tcctggcacc cggaacatcg cgtgcagctc caggcagtgt cacgaacctg
    10561 acggccaccg ccacgggaac caacgagatc acgctctcat ggacgccata ttccggcaac
    10621 gacttcgcac gctacgaaat ccggcgcggc accgcggccg gcgtgacgac ggccagcccg
    10681 ctggtagaca ccctctcggc gaaaactgcc agttcgtttg ctgaccgcac cgtcctcccc
    10741 tacacgacct actactacaa ggtgtttgta atcgacacgg ccggagtcgc cggttcatcc
    10801 gcggaaccga gcgcgcgtac tctcgccctc tcgtacccct tcgccgatac cgttgccgcc
    10861 gcgaacgtga acttcgttcc caccggcaca tgggcgatcg tcgccaattc cccgggtgaa
    10921 ggcgacgcgt acagcggatc ctggcactgg tccgactctc ccggcggtaa ctacgcaccg
    10981 aactcgaacg ggacactgac gttagccatc cgcctcggca cggccaccat gcccgtgctc
    11041 acctaccggg agcgctacgc gttcgaaacg aacgtcgatt acggccatgt ggaggtctcc
    11101 gcggacggga caaactggta cgccgtcgcg tccgtcaccg gcagccagac cacgtgggcc
    11161 gaacgccgta tagacctcac ccagtgggca gggtacgacg gtatccggat ccgctttcgc
    11221 atcgccgcga gcagttctgt ggaaagcgac ggctggcacc ttgactcttt ctccatcaac
    11281 gagacacctc tcgcctccat gccctatcct ctgcgggagg atttcgaatc cggcacgtcg
    11341 cggtggcttc tgggcggctg gtctccggcc accgaaggga cgaatgggcc gacctcgatc
    11401 cacgacagca agacaggcaa ctacccggcg cttgcgtaca acggcatggt ctccacagga
    11461 gtcttcgacc ttacgttcgc gaaaaacccg accctggtct tctggcatcg gtacaacatc
    11521 ttcaacgacc acagctacgg ccacaatgaa gacgactggg g
//
LOCUS       DAOTNG010000068        11217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5478_length_11217_cov_5.194858, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000068 DAOTNG010000000
VERSION     DAOTNG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_11217_cov_5.194858"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="P5179_04840"
     CDS             complement(<1..1030)
                     /locus_tag="P5179_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94581.1"
                     /translation="MPIPVDWLLAGEPFVEYRTRRELLDQPETDGSVRAARTAMLTDP
                     RVIALIAGLSEWPGTVIASHRSASQPFHRLNFLADLGLTVADDGVRAIANAVVEHQSD
                     EGPFQLPLDIPPQYGGPGQARWAWALCDAPLLVYALAKIGMAADSRVLRAVNFLAGLV
                     RAHGWPCVVSKELGKFRGPGRKDDPCPFATLAMLKALSQFEHLRDGPEARTGVEALLS
                     LWVESRTQHPYMFFMGKDFRKLKAPLVWYDLLHVLDVLSQFPSAAADGRMRDMVDTLR
                     AKADSEGRFAPESVWQPWSGWEFGQKKAPSRWVTLLAWRVLRRVGGESGARASDPGYA
                     VSPRWGCLR"
     gene            complement(1035..1406)
                     /locus_tag="P5179_04845"
     CDS             complement(1035..1406)
                     /locus_tag="P5179_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94582.1"
                     /translation="MDRTKRLVIATVMGFVCGFVCLGLAASGPGDVSIPVALTLITGR
                     ALIGVAIGISCFALGHWAIHGLVMGLVFSLPTGFGSMLGPEVQGFTPWSMFMWTVILG
                     GIYGVAIEFVTTVVFKAKMRE"
     gene            1619..1789
                     /locus_tag="P5179_04850"
     CDS             1619..1789
                     /locus_tag="P5179_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94583.1"
                     /translation="MKSSKPECKNYIAEDLCAASANHPEEHCHDDVKGDCGRFVPVQT
                     LETPSTEKKKTA"
     gene            complement(1910..2950)
                     /locus_tag="P5179_04855"
     CDS             complement(1910..2950)
                     /locus_tag="P5179_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94584.1"
                     /translation="MATDRELFSPLPREEVIKAVERRKPARIPLVMAKWWGEGLGDQY
                     GDRLREFDRIPDDVGMLWWGPLDVGKMGLSWQAKSGAAHDSHCVIDDWNHLDEFIEKM
                     PDPERDDAFSGLLEHAERMRKDDRYILFGWWGLFFERPWGLRGMENLMADYYEAPEHV
                     HRLHSALADLYIGYIRRAAREFQPDGFWTSDDLGHQTQSMMRREQFDALLKPYYDRVG
                     KACKEAGMHWWLHSCGNNTVLLPSLIDAGVDVFHPVQKHTMDEIAVAREFGGKITFLA
                     GIDVQHTLQEKTPEGVREEVRFLIDTFDQPDGGMCIASGNGIVSGTPFENIEAFLDES
                     VRYGAAHRRRFA"
     gene            3185..5863
                     /locus_tag="P5179_04860"
     CDS             3185..5863
                     /locus_tag="P5179_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS94585.1"
                     /translation="MFHSFLRAAALSAAFLATSAHGGTVVLNEVMASNSATITDAAGT
                     YADWIEIYNDSTDAVQLGGYGLTDTPSNPFQWVFPAVSIAPGEFLLVWASGKDSTDPV
                     GALHTNFKISSDGETIVLTRPDGTLQDSLPRAAMGKDVSWGRTPDGGADLVFFTAATP
                     GRSNAGGGQAVLEPPAFSHSGGFYPAPFDLSISSSDAQATIRYTLDGSEPTETSPVYS
                     GALRIEDRTGPASGILLIRTTNPQNVWHAPTGNVFRGTPVRARAFKTGCLPSKTVTGT
                     YFVTPKGRARYPFPVLSLVTDSLNLFDDTIGIYVPGSKYAQTGLKEDANFYQSGGDWE
                     RPVHVELMEEDGTLAFSQGAGMRIHGNYTRDYFQKPFRLYAKSSYGKDWFETRLFPES
                     DITRFKRILVRTGASDIYTALIRDGVMQSLVSHLSFDTQKYRPVVVFFNGDYWGIQFF
                     RDRYDEYYLQTHHGADPSAVDLIEIDYSLPGGPYDADPGDAVAFTDMLSYTKNHSATD
                     PQVYEHFKTLVDVENFAEYCAANIFVANTDWPHKNVRVWRARVPYSPSAGQLDGRWRW
                     LAYDFDRGFGLRESSQADQNTLIWATIGNPNQESTWLLRWLLLNPDFRTMFINTLADQ
                     LNSTFTEPRMIGMIDSLAAQIAPGVPEHIARWGEPKYLYYWTAYVQTLRNFATARRPH
                     MIQHILGYFRTYTADTAAVTINTDGPAQNCGTVRINSITIEDGFPGVSHPVYPWRGVY
                     FRDIPVRLVATPKPGYVFAGWETAAGIISLSDTLTLSLTTDTTVTAKFTNPADVAAPP
                     SRRAFYLDQNTPNPFNPSTTLRFGLPDAGHVTLAVYDVNGRLVRNLAGRTFLSGHHEA
                     VWDGCDNNGRAVASGVYLVRLTAREGVLTRRVTLLR"
     gene            complement(5948..6688)
                     /locus_tag="P5179_04865"
     CDS             complement(5948..6688)
                     /locus_tag="P5179_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRS94586.1"
                     /translation="MGFPSPVNLSVSLLVVMSSTAFASPFVGSPREYALGIGALGTAA
                     LLEQWPPNRSHPLIGGEQKPYIERERVPDWALAGAHLVTIAAIRFSDSPDRVRYAHGY
                     GMAVALTQATTSFTKGIVGRKRPNEDAAVALGKSSKSRSFFSGHASTGFCLATYSSAY
                     AWRASPKLEVRAGVPLATYGAATYAAWSRVAEHRHYPADVIAGAAAGTAIGALVFRWY
                     DGMEAGGRAGASASVFPLPRGAAVSVAF"
     gene            complement(6764..10135)
                     /locus_tag="P5179_04870"
     CDS             complement(6764..10135)
                     /locus_tag="P5179_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRS94587.1"
                     /translation="MAEEKKSPWVRHIHLWLALAAGLFFVLLSLTDFYELIELKTYDY
                     RLILRNKIFGPPHQLPNLATIDVDDYSVDKEGRFEDWDRTYHARIIDIASALGARAIG
                     VDFLMPEPSTPMIRENQVAESDVHSREAVLALFRNPDVVLSDACRKWNNVYFAQYLTE
                     AETQDYDRSLRENPPRTEVEEHRFQLVQRFTIPITQDFQKEFVVGSQLWAPVDTFLAT
                     ARGAGQVQPIPDMDGIVRRNRAFYVYDGRIFPSLSIVMAADYLGVPLSSFKFEPGRVT
                     LPNAHIPGEPAPRDIVIPLGERGTILVNWAGDYRSTYRHFPYASVKTFWEVHQREQLA
                     GLVKRDLARDPALLDGLMGGQIDLSEFTLSRMMELSRVPSVDVKMGLQDVAVALKMSG
                     AVSRRAPVTEALRDAFGKTTGIPDIGRWRALYEELLLNGRVADLFAGRPDLTLADAAR
                     DLNMPAARLESTYTKIRTLVSPLTGEIAPGNYPLWFFAQTRDGAPVAVNDSVNSVLAS
                     AMKEFLRRHGVISDAVVTGIVTPTPETGADLTNLAVRVVAFAAGYPEQDYVAVTGSIV
                     QAALLEPLVAEGTSFDDIVQQLFQVSPSDLPAEVIQPYRDTYNTLWRNNNLAAVLKAD
                     TSLSVEAAADTLTVLLRKSAGNDSVAVSAVDIVAEHAILLKHLKEQGDIPPEAHPLVF
                     NTVVVDGRPVFVSDFEGAVLFYGITSTGGHDRNPTPFEPRYPMVGMHLNLFNQVVTGG
                     FLYQAPKWLDWAVILAVALLMGLLVPKMSPVNGGILAFAMVVVFLTSTVVLFAKGRIW
                     IDTVGPFGAITFGYLVITVRNYIVGEKEKKFIKGAFSAYLAPEVVEIIANDPEGLKLG
                     GSPEHITAFFSDVKGFSTISEALSPTELVNLLNEYLTEMCDIAFKYGATVDKFIGDAI
                     VAFFGAPIKYEDHAVRACLCACEMQSRLAVLREKWKAEGKHEIYCRIGLNTGNAVIGN
                     MGGRQRFNYTMMGDTVNLAARLESGAKQFGVYTMITDVTYNAARDVIEARELDRIVVI
                     GKKEPVTIYEVLSKKGELDPKKKQIADFYAEGLAYYREQNWDAAVAKWRAAMAVDPDH
                     PDATSGIFIKRVDAIRAGKVEIPKDWNGVWEMTEK"
     gene            complement(10382..10912)
                     /locus_tag="P5179_04875"
     CDS             complement(10382..10912)
                     /locus_tag="P5179_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94588.1"
                     /translation="MRRTWCVAGMMAALGVCALSTGVHAANPDTSRSAKSAAMLMRLA
                     RAQQLSARPRTFQTQQGVAVAARRHADAMEQQKRTLAILNNQYLRGTAATSPSESDVR
                     QTIGVLEQQLNAPTPPENAYEIRYYLAACYESLDNIPKAKELLQTIVAQYGSSTDDVV
                     KGFVEQARADLARLGS"
BASE COUNT         2247 a         3657 c         3218 g         2095 t
ORIGIN      
        1 cgcgcaggca gccccaacgg ggcgaaacag catagccggg gtcggaggcc cgggcaccgg
       61 actcgccgcc cactctcctc agtacgcgcc acgccagaag cgttacccag cgagagggcg
      121 ccttcttctg cccgaactcc catcccgacc agggctgcca gacggactcc ggcgcaaacc
      181 ggccctccga atccgctttt gcgcgcaacg tatccaccat gtcgcgcatg cgaccatctg
      241 cggcggccga cgggaactgc gagagaacgt ccagcacgtg cagcaggtcg taccagacaa
      301 gtggcgcctt gagtttccgg aagtccttgc ccatgaagaa catgtacggg tgctgtgttc
      361 ggctctccac ccacaggctt agaagggcct ccacccctgt gcgggcttcc gggccgtccc
      421 gcagatgctc gaactgcgat agcgctttga gcatggccag tgtggcgaag ggacaggggt
      481 cgtccttgcg ccccgggccg cggaacttgc ccagttcctt ggagaccacg cacggccagc
      541 cgtgtgccct gaccaatcct gcaaggaagt tgaccgcccg cagaacacgc gaatcggccg
      601 ccatgccgat cttcgcaagc gcgtacacga gcaagggcgc gtcgcaaagc gcccaggccc
      661 acctggcctg gccgggtccc ccgtactgcg gcggaatgtc cagagggagc tgaaacgggc
      721 cttcgtcgga ctggtgctcc accaccgcgt tcgcgatcgc cctcacacca tcgtcagcaa
      781 cggtaagccc caggtccgcg aggaaattga gccggtggaa tggctggctg gcgctcctgt
      841 ggctggcaat gacggttccc ggccactccg agagtcccgc gatcagagcg atcacgcgcg
      901 ggtcggtcag catcgcggtg cgcgcggcac ggaccgaccc atccgtttcc ggttggtcca
      961 gaagttcgcg ccgcgtgcgg tactcgacga acggttcgcc cgcaaggagc cagtctacgg
     1021 gaatcggcac atgcttactc ccgcatcttc gccttgaaca ccactgtcgt gacgaactcg
     1081 atcgccacgc cgtagatccc gcccaggatc accgtccaca tgaacatgct ccacggcgtg
     1141 aacccctgca cctccggccc cagcatgctc ccgaatccgg ttggcaggct gaacaccagc
     1201 cccatcacaa gaccgtgaat cgcccagtgg cccagcgcga aacaactgat tccgatggca
     1261 accccgatca gcgcccgtcc ggtgatgagc gtcagtgcaa ccgggatcga aacgtcccct
     1321 ggcccgcttg ccgcaagacc aaggcacacg aacccgcata cgaaccccat caccgtcgca
     1381 atcacgagcc gtttcgtcct gtccatcaca cacctccccc gaaatggaat gaatgatgcg
     1441 accggttgtg tccgggttgc cagaaatgtt gcgggaactc agtcctgtgt caaccgaccc
     1501 tttgtggggt gagaggacgg cgccgggttc ggcgcgcctg ttctttgaca accggcatcc
     1561 gctccgctat ctttggtaca tggaaaacac gtgcgccgga acacgcggag gaaacgcgat
     1621 gaaaagctcg aaacccgagt gcaagaacta cattgccgag gatctgtgcg cggcgtccgc
     1681 caaccacccg gaagaacact gccacgacga cgtgaagggc gactgcgggc gattcgttcc
     1741 ggtgcagacg ctggaaacgc cgtcgacaga aaaaaagaag acggcgtagc acggagttcc
     1801 cgccgacacg ccgtcaagac cttcggtcat ttccttcctg tttttgctat ttctccttga
     1861 agatcgtgtc aaaccggcag cgccttccct gaacagcgcc tgacccggct caggcgaacc
     1921 tgcgtcgatg ggctgctccg taccgcaccg actcatccag aaatgcctcg atgttctcaa
     1981 aaggcgtgcc gctgacaatg ccgttccctg acgcgataca catcccgcca tccggctgat
     2041 cgaacgtatc aatgaggaac cggacctctt cgcgcacccc ttccggcgtc ttttcctgca
     2101 gcgtgtgctg tacgtcaatt cccgccagga acgtaatctt cccgccgaac tcccgcgcaa
     2161 cggcaatttc gtccatcgtg tgcttctgca cggggtgaaa cacgtcaact cccgcgtcga
     2221 tcagagatgg cagcagaacg gtgttgttgc cgcaactatg gagccaccag tgcataccgg
     2281 cttctttgca ggccttcccc acgcgatcgt agtacggttt cagcaacgcg tcgaactgct
     2341 cccgccgcat catggactgc gtctggtggc ccagatcgtc gctcgtccag aacccgtccg
     2401 gttgaaactc ccgtgcggcc cgccggatgt agccgatgta gaggtccgca agagcgctgt
     2461 gcagacggtg cacgtgctcc ggcgcttcgt agtagtccgc catgaggttc tccatgccgc
     2521 gcaaacccca cgggcgctca aagaacaacc cccaccagcc gaagaggatg taccggtcgt
     2581 ccttccgcat ccgctccgca tgctccagaa gcccggaaaa ggcgtcgtca cgctccggat
     2641 ccggcatttt ttcgatgaat tcgtcaagat ggttccagtc gtctatcaca caatggctgt
     2701 cgtgcgcggc gccactcttt gcctgccacg agagccccat tttgcccacg tccagcggtc
     2761 cccaccagag cattcccacg tcgtccggga tgcggtcgaa ttcccgcagg cggtcgccgt
     2821 attggtctcc cagaccctct ccccaccact tcgccatcac aagcggaatc cgtgccggct
     2881 ttctgcgctc cacagctttg atcacttctt cgcgcgggag gggcgaaaac agctcgcgat
     2941 cggtggccat gcgtctgttc cttcgttgga tttcgttccg caaccgggtg cccttgtgca
     3001 ccaccagaat atgcgccctt cgccccgcct gaccaaccga ccggtgggcg gcggcggtac
     3061 cccgggggcg agccggtcaa ccccttcaac ccctgaggaa tgcacggtta cccctccggt
     3121 tttgggccga atgccgtacc ttatcagtaa gaaccgtagt acatcggggg atgggagaaa
     3181 tgggatgttt catagtttct tacgggcggc agctctatcg gccgcatttc tcgcgaccag
     3241 cgcacacgga ggaaccgttg ttctcaacga ggtgatggcc tcgaacagcg caaccatcac
     3301 cgatgccgca ggcacctacg ccgattggat cgagatctac aacgacagca ccgatgccgt
     3361 ccaactgggc ggatacgggc tcacagatac cccttcgaac cccttccagt gggtattccc
     3421 cgcggtcagc attgcgccgg gggagttcct gcttgtctgg gcgtccggca aagattcgac
     3481 ggatccggtt ggcgcgctcc acacaaactt caaaatcagc tccgacggcg aaacgatcgt
     3541 tcttacacga ccggatggca cgctgcagga cagcctgccg cgcgcagcca tgggcaagga
     3601 cgtctcctgg ggccgtaccc cggatggcgg cgcggattta gtgtttttta ccgcggcaac
     3661 ccccggccgg agcaatgccg gcggcgggca ggctgttctc gagccacccg cgttctctca
     3721 ctcgggaggg ttctacccgg ctccgtttga tctctccatc tcgtccagcg acgcacaggc
     3781 gacaattcgg tacacgctgg acggatcgga gccgacagag acatcccccg tatactccgg
     3841 tgcgttacga atcgaagacc gcaccggtcc ggcatcggga atccttctca tccgtacgac
     3901 caacccgcag aacgtctggc atgccccgac cggcaacgtg ttccgcggca cgccagtccg
     3961 tgcacgggca ttcaaaacgg gctgtctgcc aagcaaaacg gtaaccggaa cctatttcgt
     4021 caccccgaag ggaagggcgc gttatccctt tcccgtgctg tcgctggtaa cggattcctt
     4081 gaaccttttt gacgacacca tcggtatcta cgtgccaggc agcaagtacg cacagaccgg
     4141 gttgaaggaa gacgccaact tctaccagag cgggggcgac tgggaacgcc cggtgcatgt
     4201 ggagctcatg gaagaagacg gaacactcgc cttctcgcag ggcgcgggaa tgcgcatcca
     4261 tggaaactac acccgcgatt atttccagaa gccgttccgc ctctatgcga aatcatcgta
     4321 cggcaaggac tggtttgaaa cgcggttgtt ccccgaaagc gacatcaccc gcttcaagcg
     4381 cattctcgtg cgcaccggcg caagcgatat ctacaccgca ctcatacgcg acggggtgat
     4441 gcagagcctc gtgagccacc tgagcttcga cacgcagaag taccggcctg tagtggtgtt
     4501 cttcaacggc gactactggg gtattcagtt ctttcgggac cggtacgacg aatactacct
     4561 ccagacacat cacggcgccg atccttccgc cgtggatctg atcgaaatcg actacagcct
     4621 ccccggcggc ccgtatgatg ccgaccccgg tgatgcggtg gcattcacgg atatgctctc
     4681 ctacaccaaa aaccattccg ccacagaccc gcaggtatat gagcacttca aaacgctcgt
     4741 ggatgtggaa aacttcgccg agtactgcgc cgcgaacatc tttgtcgcta atacggactg
     4801 gccacacaag aacgtgcgcg tctggcgggc ccgcgtcccc tattccccga gcgccggcca
     4861 gttggacggg cgctggaggt ggctggcgta cgacttcgac cgcgggttcg ggctccggga
     4921 aagctcacag gcagaccaga acaccctgat ctgggcgacc atcggcaacc cgaatcagga
     4981 atccacctgg ctcctccggt ggctcctgct caaccccgat ttccgcacga tgttcatcaa
     5041 cacgcttgcc gaccaactga actccacgtt cactgaaccg cggatgatcg gcatgatcga
     5101 ttctctcgca gcgcagatcg cacccggagt gccggagcac atcgcacggt ggggcgagcc
     5161 aaaatacctg tactactgga ccgcatacgt tcagacgctc cgcaatttcg cgaccgcacg
     5221 ccggccgcac atgatccagc acatcctggg ctacttcaga acctacacgg ctgacaccgc
     5281 ggccgtaacc atcaacacag acggtccggc acagaactgc gggacggttc ggatcaacag
     5341 catcacgatc gaggacgggt tccccggggt ttcgcatccg gtgtacccgt ggcgcggcgt
     5401 ctacttccgt gatatcccgg tacgcctcgt ggcaacgccg aaacccgggt acgtattcgc
     5461 cgggtgggag accgccgcgg ggatcatctc cctcagcgac accctgacgc tctccctgac
     5521 cacggacacg acagtgacgg cgaaattcac gaatcccgcc gacgtcgccg cgccgccgtc
     5581 ccggcgtgcg ttttacctcg atcagaacac gcccaacccg ttcaacccgt ccaccacgct
     5641 gcgcttcggt ctgcccgacg ccggacatgt gacgctggca gtgtatgatg tcaacggcag
     5701 actggtgcga aatctggcgg gccggacatt cctgtctggc caccatgaag cagtttggga
     5761 cggttgcgac aacaacggca gggccgtcgc cagcggcgtg tatctggtgc ggctgaccgc
     5821 gcgcgagggc gtgttgacga gaagggtgac cctgctgcgc tgaagtgaag ttcaaacggg
     5881 gaaagagcgc gcgaagaggg gttcccggag aacgcaggcg ttcctccgca ggcggcttgt
     5941 tgccgcctca gaacgctacc gacaccgcgg cgccccgcgg cagagggaac acgctggcgg
     6001 aggcgcccgc ccggccgccc gcctccatcc catcgtacca gcggaataca agtgcgccga
     6061 tggccgtgcc ggcggccgcc ccggcaatta catcagcggg atagtgccgg tgttcggcca
     6121 cgcggctcca cgccgcatac gtcgccgcgc cgtacgttgc gaggggaaca cctgctctga
     6181 cctccagctt cggcgaggca cgccatgcgt acgcggagga atacgtggcc aggcaaaatc
     6241 ccgtactcgc gtgtccgctg aagaaactcc gcgatttgct gctctttccc agagcaaccg
     6301 ccgcatcctc gttgggccgc tttcgtccga cgatcccctt cgtgaacgac gtggttgcct
     6361 gcgtcaacgc aactgccatc ccgtagccat gggcataccg taccctgtca gggctgtccg
     6421 aaaagcggat tgccgcgatc gtgacgagat gcgcccctgc cagcgcccag tcgggcacgc
     6481 gttcccgttc gatataaggc ttctgttctc cgccgataag cggatgggaa cgattcggcg
     6541 gccactgttc cagcagggcg gccgtgccca gcgcgcctat tccgagtgcg tattcgcggg
     6601 gggaaccgac gaaaggagaa gcaaaggccg tggatgacat aaccaccagc agcgaaacgc
     6661 tgaggttcac gggtgaaggg aatcccatgg ggccgtcccg gaaaaacaca ccccctccgc
     6721 cgcggtacga gtcggagggg gtgatgtgca acgctcctgc gatttacttc tccgtcattt
     6781 cccagacgcc gttccaatcc ttcgggatct ccaccttccc cgcgcgtatc gcatcgacgc
     6841 gcttgatgaa gatgccggag gttgcgtccg gatggtcggg gtcaaccgcc atggcggcac
     6901 gccactttgc caccgcggca tcccagttct gttcgcggta gtacgcaagg ccttctgcgt
     6961 agaagtccgc gatctgtttc tttttggggt cgagttcgcc cttcttggac agaacctcgt
     7021 agatggtcac cggctctttt ttgccaatca cgacgatcct gtcaagctca cgggcttcga
     7081 tcacgtcgcg agccgcgttg taggtaacat cggtgatcat cgtgtagacg ccgaactgct
     7141 tcgcgccgct ttccaaccgc gcggcaaggt tcaccgtgtc gcccatcatc gtgtagttga
     7201 accgctgacg gccgcccatg ttgccgataa ccgcgtttcc ggtgttaagc ccgatgcggc
     7261 agtagatctc gtgcttgccc tccgccttcc atttctcacg aagcaccgcc agccgcgact
     7321 gcatctcgca cgcgcagagg cacgcccgga ccgcgtggtc ttcatacttt atgggcgccc
     7381 cgaaaaacgc gacgatcgcg tcgccgatga atttgtccac cgtcgcgcca tacttgaacg
     7441 cgatatcgca catttcggtc aggtactcgt taagcagatt caccagctcg gtcgggctga
     7501 gcgcttccga aatcgtggag aatcccttta cgtcgctgaa aaacgccgta atatgttccg
     7561 gtgatccccc gagcttcaac ccttccgggt cattggcgat gatttccacc acttccggcg
     7621 caagatatgc cgagaaggcc cctttgatga acttcttctc tttctctccc acgatgtagt
     7681 tgcggacggt gatcacgaga tacccgaacg tgatcgcgcc aaacggcccc accgtgtcaa
     7741 tccagattcg ccccttggcg aacagcacca ccgtgctcgt caggaacacc accaccatgg
     7801 caaatgcgag gattccgcca ttcacggggg acattttggg cacgagaagc cccatgagaa
     7861 gcgcaactgc cagaattacc gcccagtcca gccatttcgg agcctgatac aggaacccgc
     7921 cggtcacgac ctggttgaac aggttcagat gcattcccac catcgggtag cgcggctcaa
     7981 agggggtggg gtttctgtca tgcccgccgg tggaggtgat gccgtagaac aacacggccc
     8041 cttcaaagtc ggatacgaac acgggtctgc cgtccaccac aaccgtgttg aacacgagcg
     8101 ggtgcgcctc cggcggaatg tccccttgtt ccttcagatg cttcagcagg atcgcatgct
     8161 cggccacgat gtcaacagcc gaaactgcga ccgagtcatt tcccgcgctc tttcggagca
     8221 gaacggtcag cgtgtcggcc gcggcctcca cggaaaggct cgtgtccgcc ttcaggacgg
     8281 ctgcaagatt gttgttcctc cagagcgtgt tgtaggtatc gcggtacggc tgtatcactt
     8341 cggccggcaa atctgaggga gacacctgga aaagctgttg cacgatgtcg tcgaacgacg
     8401 tgccctcggc caccaggggt tcgagcagcg cggcctgaac gatacttcct gtcacggcga
     8461 cgtaatcctg ctccgggtat cccgccgcaa acgcgaccac acgtaccgcg aggttggtaa
     8521 gatcagcgcc ggtttcggga gtcggcgtca cgatccccgt caccaccgca tcgcttatga
     8581 caccgtgcct ccgaaggaat tccttcatgg cgctcgccag cacgctgttc acgctgtcat
     8641 tgactgcgac gggagcaccg tcccgcgtct gcgcgaagaa ccagagcggg taattgcccg
     8701 gtgcgatctc cccggtgagt ggagaaacga gcgtacgaat cttcgtgtac gtgctctcca
     8761 gccgcgcagc cggcatgttg agatcccgcg cggcatcagc aagcgtcagg tcgggacgcc
     8821 cggcgaacag gtccgccacc cgtccgttga gcaggagttc ctcgtacagg gcacgccaac
     8881 gcccgatgtc gggtatgccg gtcgttttgc cgaatgcatc tcgcagcgcc tcggtcaccg
     8941 gtgccctgcg ggaaaccgcg ccgctcatct tcagggccac ggctacatcc tggaggccca
     9001 ttttgacgtc caccgacgga acgcgcgaaa gctccatcat tcggctgagc gtgaactccg
     9061 aaaggtcaat ctggccgccc atgaggccat caaggagcgc gggatcgcgc gcaaggtcgc
     9121 gtttcacaag accggccagt tgctcgcgct ggtgaacttc ccagaaggtc ttgacactgg
     9181 catacgggaa atgccggtag gtgctccggt agtcccccgc ccagttcacg agaattgtcc
     9241 cgcgctcacc cagcggaatg acaatgtcgc ggggcgccgg ctcgcccgga atatgagcgt
     9301 tcggcagggt gacacgtccc ggctcaaact tgaatgagct caggggaacg ccgaggtagt
     9361 ccgcggccat cacgatcgaa agggaaggga aaatccggcc atcgtacacg tagaacgccc
     9421 ggttccgacg cacaataccg tccatatccg gaatcggctg aacctggccg gcgcctcggg
     9481 ccgtcgccag gaacgtatcc accggtgccc agagctgaga cccgaccacg aactccttct
     9541 ggaaatcctg cgtgatcgga atcgtgaacc gttgcacgag ctggaaacgg tgctcttcca
     9601 cctccgtgcg cggcggattc tcccgaagcg agcggtcgta atcctgcgtc tccgcctcgg
     9661 tcagatactg cgcgaagtag acgttgttcc acttccggca cgcgtcagac agcacgacgt
     9721 ccgggttgcg gaaaagtgca agcactgctt cccgcgagtg aacatcgctc tcggcaacct
     9781 ggttctcccg gatcatgggt gtgctgggtt ccggcatgag gaaatcaacg ccgatagccc
     9841 gcgcacccag cgccgatgcg atgtcaataa tccgcgcgtg atatgtgcgg tcccagtcct
     9901 caaaacgccc ttccttgtcc acggagtagt catctacgtc aatcgtggca agattgggga
     9961 gctggtgcgg cgggccgaaa atcttgttcc ggagaatcag tcggtaatcg tacgtcttca
    10021 gttcgatcag ttcgtagaaa tcggtcaacg agagcagcac gaagaacaga cccgcggcaa
    10081 gcgcaagcca gagatggatg tgacgcaccc agggtgattt cttttcttca gccattcgat
    10141 acccccttgg cgaacgggct ggttccgaca ggccccggcc tccggtggag gaggacgcac
    10201 gcattcaaag gaagaaagaa acggataacg cgcgccgacg caaagggata acccttgcca
    10261 gcgccggaaa aggggaaaat tgcggggtgg aagggaggaa acacgcgggc atcgacggcg
    10321 cgtgtcaaaa aaaagagggg ggcaacgcac gttgcccccc tcttttcaac cgtaccggct
    10381 gtcagcttcc cagtcgcgca aggtccgcgc gtgcctgctc gacgaagccc tttaccacat
    10441 cgtccgtgct ggagccgtac tgagccacta ttgtctgaag cagctctttc gccttgggaa
    10501 tgttatcaag cgactcataa cacgccgcaa ggtagtaacg aatttcgtac gcgttctccg
    10561 gcggcgtcgg tgcgttcaac tgctgctcca gaacgccgat cgtctgtcgg acgtcactct
    10621 ccgaagggct ggttgcggcg gtgccgcgga gatactgatt gttcagaatc gccagcgtgc
    10681 gcttctgttg ctccatggca tcggcgtggc gacgcgcggc aactgcgact ccctgctgcg
    10741 tctggaacgt cctgggacga gccgacagtt gctgcgctcg cgccagacgc atcaacatcg
    10801 ctgcactctt cgcgcttcgg gaggtatcag ggttggcggc atgcaccccc gtgctgagag
    10861 cacacactcc cagagccgcc atcatccctg caacacacca cgtgcgtctc atcgtacacg
    10921 cctcctgaaa aaaatgaaaa ctgttcaaat caaagtgctc tttgcaaaag tgcgctaccc
    10981 gtaagacaag tgtacgcgtt ccttcacaaa agtgtaccgt tgagcacaat agaaaatata
    11041 cggcgccagg atttttccgc aagtgatgcc tgttccgcgc cctgtggagc ggcatttcat
    11101 ggcagcagta cagcctcgtc attgcccggt ccggaaaata tcctccagta ccggccggag
    11161 ttcttcgtca atctgcagtg cctgctcccg tgcctgctgt gcttcctgcg tatcgtt
//
LOCUS       DAOTNG010000069        11086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5545_length_11086_cov_5.156468, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000069 DAOTNG010000000
VERSION     DAOTNG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5545_length_11086_cov_5.156468"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1242
                     /locus_tag="P5179_04880"
     CDS             229..1242
                     /locus_tag="P5179_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS94589.1"
                     /translation="MRRRVFLWIGAASSILFLYIALRNIAFDQFLSAFKSVDVLPVSG
                     AFLILLLVYVVRTLRWRMIIESTERISNWRTFSTLMIGFFANNVLPARAGELIRAVIL
                     RRQIGVTRSFALGTIVVERVMDVAALVGLLLLAIYRVPPDRLPAITSEVRGFALGTLA
                     LVIAGMCVLLWGKDTVVALLESLFSKIMASHRAGILAGKFEQLAVGLEVMRDPSRLVF
                     VAFLSFLSWVGMAAVFYFVFVGFHLDVTPAVAAFTVSLVNLGMAVPSSPGYVGTYEFF
                     MVVSLGAFGIGKATALAYGLVTRLLWYVFEVVVGFACLWKSELSIRELFHGEESAIPG
                     DSG"
     gene            1405..4863
                     /gene="mfd"
                     /locus_tag="P5179_04885"
     CDS             1405..4863
                     /gene="mfd"
                     /locus_tag="P5179_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRS94590.1"
                     /translation="MTLDQIRVRIAASDPAQALLRAISENRPEIPFHGASGSLPAFLL
                     AHLRKTSNRQILAVTPTTETAEELRDDLESILGEEAVRYFPDWELIAFEEKSPHVEVT
                     ALRLEALQSLLVGEPVVIVAPVVALLRPTLEPEALAAASRVIRVNELLDMDELAKWLR
                     SLSFEPSNQVQEMGQYARRGGIIDIFTFGSENPYRVELFDDEVESIREFDPATQRSVR
                     EVNEMEILPRREILAGSEWWGNAAERVKRAEDAYGVSLDYVRNKLEFGIHFEGIEFWC
                     PVLTGTKPSVLDHLDSRALVFLSDPESITEQAEKFARETARLVSRRIERGEPTLPPGA
                     VFHSWQNLRETIAPRCLVSNHSLSPPGAVASFGAQSARRYDSSLEAVRADVDTFAERG
                     FSVVLFAENESQARRLEDLLGSSMDRIAVEIGKLHGGFVYPSAKLFVVNDHELFSRYQ
                     GRHRFRKFRGATPVTDVFALGPGDLVVHEEHGIGVYQGLERITVDGVSRDCLRILYRD
                     QDRLFVPADQINRVQRYSQDDSARPVLSKLGGSDWDRVKERTRKGVLKLAQELIELYA
                     IRQSRPGFAFSPDSTLMEEMEAAFIYEETRDQLRAISQIKGFMESPVPMDLLLCGDVG
                     YGKTEVAIRAAFKAACDGKQVAVLVPTTILAQQHFRTFSERLAGFPVKVDVLSRFRSK
                     HETEETLYQLKRGAIDIVIGTHRLLSDDVEFRDLGLLVIDEEHRFGVRHKERLKHMRQ
                     TVDVMSMTATPIPRTLYLSLMGARDMAVISTPPRDRLPVHTEVIPFDEDRISEAILRE
                     VARSGQVFFVHNRVQTIEERAEFLRELLPELQFRVAHGQMSEHQLEKVMLDFLEHRFD
                     CLVTTMIIQSGLDMPNVNTILVDRSDQFGLAELYQLRGRVGRSNHKAFAYLMVPKDRK
                     LPPESRRRLRAIEEFSDLGSGFKVAMRDLEIRGAGNLLGNEQSGFIAAIGFEMYVKLL
                     DDAVKELKGEIPERTEDCEVDVHVSAFLPDTYVGDGEQKMTLYRRLAESQTLAEVNEF
                     TDELRDRFGRPPEPASALIDLMKVKVAGRILGARRIAVDKHGKIRIVLGDGRKMTSTQ
                     IGDLVRRCSEPVEFKSNGDLVMEIQSGEPGGPAQAARVAAILESLIEEDASEAKKPVR
                     LVWGER"
     gene            4860..5756
                     /locus_tag="P5179_04890"
     CDS             4860..5756
                     /locus_tag="P5179_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS94591.1"
                     /translation="MRRMTARLVPGAWLIAAGILAGCGWFQSQDGEPVAVVGDVKLTQ
                     GDLDRMLANDAFEEKSVEARRRAVTRWVNRELLYQEALRRECDRDPIVRFQLETARRD
                     ILVNALIDRSQRDSPVTEEQITRFYEENRNIFKRTEAMIHVRQIVLEDPKEARRVASE
                     VQRRPETFAEVARARSSDPSSADGGDVGFVTARTAYSPEVWQVLQRLSDNEISRPIPI
                     DAGWIILQAVEHRPAGSIKSLDEVRSEVVNRLRAAERANLMAALAESLKLKEDVEVFA
                     DRIVPAAGVLRSPGSRTPVGAE"
     gene            5826..7160
                     /locus_tag="P5179_04895"
     CDS             5826..7160
                     /locus_tag="P5179_04895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS94592.1"
                     /translation="MRKNRPLCCALLLIVTSLLSFRTSFAAEEVVDRILAVVERDVVL
                     QSEVDEYITLQMIQAGRSIQSLSAPERDQLRCQILQAMVDDRILVAKARADSVVVTLQ
                     QVEDALRQQLDNIKGQFPSEEAYREQLVREGTTERDLRAKLRLQMERYLLREKLTQTY
                     GQKITVTFNEMERFYNQYRDSLPVVPASVTIAHITRVTKPGDSALVAARRVLDAARER
                     LAAGEDFASVARALSQDPGSAQFGGDLGFFGRGQMVPEFETAAFSLDSGQVSGPVLTE
                     YGLHLIQNLGFRGEQVHARHILARAVFTPADRQATLDTLQSVYRRLQAGQDFGELARI
                     YSMDQSVRQTGGRIGPVDPSALPPAFGRVLATLSVGETSQPFESDPGTFHIVRLLERT
                     REHAMNMNDDRRQIEEMVRQRKLQTRINEILEQERGRMYVDVRLPGCFPSAR"
     gene            7166..7465
                     /locus_tag="P5179_04900"
     CDS             7166..7465
                     /locus_tag="P5179_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRS94593.1"
                     /translation="MPAAADTKGAVLRMDSVLFRLRLVSSREQAKRACENGIVELNGK
                     VAKPSSSVRSGDFIRVRFTDEDLRFTLIELPRKSMSKTSARSCYAVTASNAVERG"
     gene            7469..8239
                     /locus_tag="P5179_04905"
     CDS             7469..8239
                     /locus_tag="P5179_04905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS94594.1"
                     /translation="MAFLRRDVIEKPPYDQLAALYDYVMRHVDYVEWADYIEQLFRWC
                     GANPHKITEAACGTGALALILADRGYSVSGYDLSEAMISVAKAKARAGGENLPQFAVA
                     DMKSLPHGEADVVLCMYDSINYCSNTGELAATFRSFRKAVRAGGIAIFDVTTEANSLR
                     YFRDYRYRERHDGYMISRHSVYHPETRTQTNEFVFEGPDLPGRVKEMHRQQIFSLKEV
                     LESIPAFEWSVLGAFDGYTLDPPDDDTERIHIVMRALG"
     gene            8255..8932
                     /locus_tag="P5179_04910"
     CDS             8255..8932
                     /locus_tag="P5179_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS94595.1"
                     /translation="MGDTPFLAFDAVTLQGRRAPIVEDVSLSLAAGGFGVLFGPTGGG
                     KTTILRLAHLELRPTVGRVKVGDISSDHLNAGEAAIARRAIGMVYQDCRLLSDRSASE
                     NIILPLQIAGMATGMARRRALEVLGQVGLAGKRSISPRYLSGGELQRLALARALAMDP
                     HLLLADEPTGNLDSDTAEEIMTLIRRINARGTTVLLATHDLKLIGGYGYPCWRVEDGH
                     AVQEEPR"
     gene            complement(9063..9368)
                     /locus_tag="P5179_04915"
     CDS             complement(9063..9368)
                     /locus_tag="P5179_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS94596.1"
                     /translation="MPDHFDTVDHVAVVVDDIGQAVNWYSSRFRCEVAYQDDTWALVR
                     FANISIAFVLPGHHPRHVGIHREDIEQFGNVGVHRDGVRFVYLEDVSGNVVEVVKRP"
     gene            complement(9392..10207)
                     /locus_tag="P5179_04920"
     CDS             complement(9392..10207)
                     /locus_tag="P5179_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94597.1"
                     /translation="MSAFDVSTILKSVSAMQEKPPRLWWSTDEASHMRSRLLCDNRYA
                     ESVEAHAESVIRDPDVFAREPAWHVSQCEPLLSLAYAAWLFQRERYASLASRLMDRAA
                     SAESWDAPDEFSPKPVAVALTLAKAIDLLAAFTTDDQLERYDIAAREKCFARLPGILK
                     SRGMTDQERMVWCSDSACAALGTLSPSGLLIQEVARAASCLMAALEKKSDVRALPVVR
                     IGRALSRRSDDVARLMRDQGLNLAIPEPNPFAVFDCEVLALLELPVACIPSAA"
     gene            complement(10247..10888)
                     /locus_tag="P5179_04925"
     CDS             complement(10247..10888)
                     /locus_tag="P5179_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS94598.1"
                     /translation="MLVEQIRTGGDRNFSYLVADETRRVCAVVDPSFAPDLVAEKAAT
                     LGFRVEYVLETHDHFDHTNGNARLKELTGAQVVMHRMSEGNCDVRVDNGDVLPLGELS
                     VTVIHTPGHTRGHACYFVEDAVFTGDTLFVGKVGGSDFGEGARQEWESLHTKLLSLPD
                     NTRVFPGHDYGVRPFSTIGDERRSNPFLLQPDFPSFLKLKMNWAEYKKLHGIK"
BASE COUNT         2276 a         3038 c         3450 g         2322 t
ORIGIN      
        1 ttacaacgag gatggcagca tcgccgactg gtgacggggc actgcctgac cgacgcgaaa
       61 aaccgattgt gctgccgtac cccggcagcc gatgattcgt gccgcgcctg tgaacgacaa
      121 cgtggtgttg gacaggcgcg gtttctttcc atgatcgctg ttgaaggcgg caggaccttc
      181 gctcgtcagc tttgcctgcc agaacgccgc catcgaagcg gaggttgagt gcgcagaagg
      241 gtcttcctgt ggatcggagc ggcgtccagc attttgtttt tgtacatcgc tctgagaaac
      301 atcgcgttcg accagttcct cagcgcgttc aagagcgtgg acgtattgcc ggtttctggc
      361 gcgtttttga tcctgctcct tgtgtacgta gtgcgcacgt tgcgatggag gatgatcatt
      421 gaatccaccg aaagaatcag caactggcgt acgttcagca ctctgatgat cggttttttc
      481 gcgaacaacg tccttcccgc acgagccggg gagttgatcc gcgcagtcat tttgcggcgt
      541 cagatcggtg tgacgagaag cttcgccctc ggcacgatcg tggtggaacg ggtgatggac
      601 gttgcggccc ttgtgggatt gcttttgctc gccatctacc gggttcctcc agatcgcctg
      661 cccgcgatca cttcggaagt gcgagggttc gcgctgggaa cgctggcgct cgtcatcgcg
      721 gggatgtgcg ttcttctgtg ggggaaggac acggtggttg cgttgctcga gagcttgttc
      781 tcgaagatca tggcgtccca cagggcaggg atactggcgg gcaaattcga gcaactggcc
      841 gttggactgg aagtgatgcg cgaccctagt cgtctggtgt tcgtcgcgtt tctgtcgttc
      901 ctaagctggg ttgggatggc tgcggttttc tacttcgtgt ttgtcggatt ccatcttgac
      961 gtgacaccgg cagtggcagc gttcactgtc tcgttggtaa accttggaat ggccgtgcct
     1021 tcgtctccgg ggtacgttgg tacgtacgag tttttcatgg tggtgtcgct tggtgcgttc
     1081 gggatcggaa aggcgacagc actggcgtac ggcctggtga ctcggttgct atggtatgtg
     1141 ttcgaggtcg tagtgggttt cgcgtgtctt tggaaatcag aactgtcgat tcgggagctt
     1201 tttcacggtg aagagtccgc gattcccggc gattcaggtt gaaaagaagc tgcgccgctt
     1261 ggacttctga gtcggataag tttccttatg atacgtgatc cccgggtttt ttcgggggca
     1321 ctttcgcgat cgagaagccg atgatcatgg acgatcaccg gcgttttgct gtttatgtcc
     1381 gcccgcttga accgaaggac cgctttgacg cttgatcaga tacgagttcg catcgctgca
     1441 agcgatccag cccaggcgct gctccgagcc atttccgaga accgccctga aatccctttt
     1501 cacggcgcat ctggttcgct tcccgcgttt ttgctcgcgc acctcagaaa gacgagtaat
     1561 cgccagatcc ttgcggtaac gccgacaacc gaaaccgcgg aggaattgcg agacgacctc
     1621 gagtcgattc tgggcgaaga agcggtacgg tatttccctg actgggaact catcgcattc
     1681 gaggaaaagt ctccccatgt cgaagtaacc gcgctccgac tcgaagcgtt gcagtcgctt
     1741 ctcgtcggcg aaccggtggt catagtagcc ccggtcgtgg ccctcttgcg tccgacactg
     1801 gaacctgaag ctctcgcagc cgcgtctcgc gtcattcgag tgaatgagct gcttgacatg
     1861 gacgaactcg cgaagtggtt gcggagcctc agttttgaac cttcgaacca ggttcaggaa
     1921 atgggtcaat atgcacgccg cgggggcatc atcgacatct tcaccttcgg ctctgaaaac
     1981 ccgtaccgtg tcgagctttt cgatgacgaa gtggaatcca tccgggaatt cgacccggcg
     2041 acccagcgtt ccgtgcgcga agtcaacgaa atggagatcc tgccgaggcg ggagatcctt
     2101 gcgggaagcg aatggtgggg aaacgccgcc gaacgtgtga agcgagcgga agatgcctac
     2161 ggtgtgagtc tggattacgt cagaaacaag cttgagttcg ggattcattt tgaagggatt
     2221 gagttctggt gccctgtatt gacgggcaca aaaccgagcg tgctggacca tctcgattcc
     2281 cgtgcgctgg tgttcctgtc ggaccctgaa agcattacgg aacaggctga aaaattcgcc
     2341 agggaaactg cgaggctggt cagtcggagg attgagcgcg gggaaccaac cctgcctccc
     2401 ggcgcagtgt ttcacagttg gcagaatctg cgggaaacaa tagcgccccg atgtctggta
     2461 tccaaccatt cactttcgcc tccgggggca gtggccagtt tcggtgcgca aagtgcacga
     2521 cggtatgact ccagtctcga agcagtgcgc gccgacgtcg ataccttcgc ggagcgcggg
     2581 ttttcggtcg tcttgtttgc ggagaacgag agtcaggcac ggagactgga ggacctgctg
     2641 ggttcttcga tggatcgcat tgccgtggag atcggcaaac tccacggcgg gttcgtgtat
     2701 ccatccgcca agctgttcgt ggtgaacgat cacgagttgt tcagcaggta tcagggacgg
     2761 cacagattca ggaagtttcg cggcgcaaca cccgtaacgg acgtgttcgc gctcggtccg
     2821 ggcgacctgg tggtgcacga agaacacggg ataggcgtgt accaggggct cgaacggatt
     2881 acggtggacg gcgtttcccg ggattgcctg cggattcttt accgcgacca ggaccgcctt
     2941 ttcgtccctg cggaccagat caaccgtgtc cagcggtatt cgcaagacga cagtgcgcgc
     3001 ccggtccttt ccaaactggg cggttctgac tgggaccggg tcaaggagag aacccgcaag
     3061 ggtgttttga aactggcgca ggagctcatt gagctgtacg caattcgcca gtcccgcccg
     3121 gggtttgcct tcagccccga ttcaacgctc atggaggaga tggaggccgc attcatctac
     3181 gaggagactc gcgatcagtt acgcgcgatc tcgcagatca aaggattcat ggaaagcccc
     3241 gtgccgatgg atctcctcct ttgtggggac gtcgggtacg gcaaaaccga ggtagccata
     3301 agggcggctt tcaaagcggc gtgcgacggc aaacaggtcg ccgttcttgt accgaccacc
     3361 atcctcgcgc agcaacactt tcgaacattc tcggagcgcc tcgccgggtt cccggtcaaa
     3421 gtcgatgtgc tgagccgttt tcgttccaaa cacgaaacgg aagagactct gtaccagttg
     3481 aaacggggtg caattgacat cgtgatcggt acgcaccgcc tcctctctga tgacgttgag
     3541 tttcgtgatc tcggtctgct ggtgatcgac gaggaacacc gcttcggcgt acgccataag
     3601 gaacgtctga aacacatgag gcagactgta gacgtcatga gcatgacggc cacgccgatt
     3661 ccgcggactc tctacctgtc gctgatgggt gcgcgggaca tggcggtaat cagcacgcca
     3721 ccgagagaca ggttgcccgt tcacaccgag gtgattccgt tcgacgagga tcggatatcg
     3781 gaagcaatac tcagagaagt cgcgcgctcc ggacaggttt ttttcgtcca caaccgggtg
     3841 cagacgatcg aggaacgtgc cgagtttctg cgagagctgc tgcctgaatt gcagttccgc
     3901 gttgcccacg ggcaaatgag cgagcaccaa ctggaaaagg tgatgctgga cttcctggag
     3961 caccgcttcg attgcctcgt cacgaccatg atcatccagt caggattgga tatgccgaac
     4021 gtcaatacga tcttggttga tcggtcagat cagttcggtc tcgccgagtt gtaccagttg
     4081 cgaggacgcg tcgggaggag caaccacaaa gccttcgcgt acctgatggt tcccaaagat
     4141 cgcaagttgc cgcccgaatc tagacgaaga ctgcgcgcga tcgaggagtt cagcgacctg
     4201 ggcagcgggt tcaaagtcgc gatgcgcgat ttggagatcc ggggtgcggg caatctgctg
     4261 gggaacgaac aaagcgggtt catagcagcg atcggtttcg aaatgtacgt gaagctgctg
     4321 gacgacgcgg tgaaggagct gaagggcgag attccggaac gaacggaaga ttgcgaagta
     4381 gacgtgcatg tcagcgcgtt tctgcccgac acgtacgtgg gtgatggaga gcagaagatg
     4441 accctgtacc ggcgccttgc agaatcgcag actctcgcgg aagtaaacga gtttaccgat
     4501 gaactgcgcg accgtttcgg ccgtcctccg gaacctgcaa gcgccctgat tgatctaatg
     4561 aaggtcaaag ttgcaggaag gatcctcggc gcacgcagga ttgccgtgga taagcacggg
     4621 aagattcgga ttgtcctcgg agacggcagg aagatgacat caacgcaaat cggggatttg
     4681 gtgcgccgat gttccgaacc ggttgagttc aagtccaacg gtgatctggt catggagata
     4741 cagtcggggg agccgggcgg accggcgcaa gccgcgcgag ttgccgcgat tctggaatcc
     4801 ttgatcgaag aagacgcttc cgaggcaaag aagcccgtgc gacttgtctg gggggaaaga
     4861 tgagacggat gacagcccgt ctggtgccgg gcgcgtggct gatcgcggcg ggaatccttg
     4921 cggggtgcgg ctggttccaa tcgcaagatg gagaacctgt tgccgtggtg ggggacgtga
     4981 agctcacaca gggtgatctg gacaggatgc ttgccaatga cgcgttcgag gagaaatcgg
     5041 tcgaagcacg aaggcgagcc gttacgcgat gggtaaatcg cgagcttctg taccaggaag
     5101 cgttgcggag agaatgcgac agggacccga ttgtcaggtt ccagctcgag acggcacggc
     5161 gtgacatcct ggtgaacgcc ctcatcgacc gttcgcaacg cgattccccg gtcaccgagg
     5221 aacagatcac gaggttttac gaggaaaaca ggaatatctt caagcggact gaagcgatga
     5281 tccatgtccg gcagatcgtg ttggaagacc cgaaggaggc gcgacgcgtg gcctcggaag
     5341 tccaacgcag gcccgagacg ttcgcagagg ttgcccgcgc gcgttccagc gatccgtcaa
     5401 gcgcggacgg aggcgacgtc ggatttgtga cggctcgaac cgcatacagc ccggaggtgt
     5461 ggcaggtatt gcagcggctt tccgacaacg agataagccg cccgatacct atagacgccg
     5521 ggtggataat tctgcaggca gtcgagcacc ggccggcggg cagcatcaaa tcgcttgacg
     5581 aagtccggtc agaggtagtg aaccgcttgc gggctgcaga acgagcgaat ctgatggcag
     5641 ctctcgcgga gagcctgaaa ctgaaagagg atgtggaggt gttcgcagac aggattgttc
     5701 cggcggccgg cgtgttgcgc agcccgggtt cgcggactcc tgtcggagcg gaatgaacga
     5761 gtcgtcacgt gttgagtgct tcgggaatgc aatgatccga atcttccttg tggaaaggtt
     5821 actctgtgcg gaaaaacagg cctttgtgtt gtgcgttgct gctgatcgta acaagtctgc
     5881 tgtcctttcg aaccagtttc gccgcggaag aggtcgttga ccgcattctt gcggtggtcg
     5941 agcgcgacgt ggtattgcag tccgaggtcg acgaatacat caccctgcag atgatacagg
     6001 cggggcgcag catccagtct ctgtcagccc cggaacgcga tcaattgcgt tgccagatac
     6061 ttcaggcgat ggtggacgac cggatactcg tggcgaaagc gcgcgcggac agcgtcgttg
     6121 ttaccctgca acaggtagaa gacgcgctcc ggcaacagct ggacaacatc aaaggccagt
     6181 ttccgagtga ggaggcctac cgggaacaac tggtcaggga aggaacaacg gaacgcgact
     6241 tgagggcgaa actacggctt cagatggaac gatatctgct cagagagaaa ctgacgcaga
     6301 cctacgggca gaagatcacc gtgacgttca acgaaatgga gcgtttctac aaccagtacc
     6361 gcgatagcct tccagtggtg ccggcttcag taaccatcgc ccacataaca cgcgtaacga
     6421 agccagggga ttccgcgctt gttgcggcgc gccgggtgct cgatgcggca cgtgagcgtc
     6481 tggctgccgg agaggacttc gcttcggttg cgcgggctct ttcacaggat ccgggaagtg
     6541 cgcaattcgg gggtgatctc ggctttttcg ggcgaggcca gatggttccg gagttcgaaa
     6601 cggcggcgtt ttccctcgat tccggtcagg tgagtgggcc ggttctcacg gaatacggac
     6661 tgcacctgat ccagaatctc ggatttcgcg gcgaacaagt gcacgcgcgg cacatcctgg
     6721 cgcgcgcagt gtttaccccc gcggatcgcc aggcgactct ggacacgctt cagtcggtgt
     6781 accgccgcct gcaggcgggg caggatttcg gcgaactggc gcggatctac tcgatggatc
     6841 agagcgtgcg gcagaccggg gggcgcatcg ggccggtgga tccctccgcg ttgccgccgg
     6901 cgttcggtcg ggttctggcg acgttgagtg tcggcgaaac cagccagccg tttgagtctg
     6961 atcccggcac gtttcacatc gtgcgcctgc tcgagcgcac ccgcgaacac gcgatgaaca
     7021 tgaatgacga tcgaaggcag attgaggaaa tggtgcggca gcgcaagctg cagacgcgca
     7081 tcaatgagat cctcgaacag gaacgtggtc gcatgtacgt ggatgtgcgg ctgccaggct
     7141 gcttcccttc ggcacggtaa tggcggtgcc ggcggctgca gatacgaagg gcgcagttct
     7201 gcggatggac tccgttctct tccgtctgcg cctcgtttca tcccgcgaac aggcgaaacg
     7261 cgcgtgtgaa aacggaatcg tagagctcaa tggcaaggtg gcaaaaccgt catcgtcggt
     7321 tcggagtggg gatttcattc gggtgcgttt caccgacgag gatttgaggt tcacgctgat
     7381 cgaattgccg cgcaaatcta tgagcaagac atcggcgcga agttgttatg ccgtcactgc
     7441 cagcaatgct gtggagagag ggtgattcat ggcattcctg cggcgcgacg tgatagaaaa
     7501 acccccctat gaccagctcg ctgcattgta cgactacgtg atgcggcacg ttgattacgt
     7561 ggaatgggcg gattacatag agcaattgtt ccgttggtgc ggagcaaacc ctcataaaat
     7621 aaccgaagcg gcgtgcggaa ccggggcgct ggctctgatc ctggcagacc gcgggtactc
     7681 cgtttccggg tacgatctgt ctgaagcgat gatttccgtg gcaaaggcaa aggcccgtgc
     7741 gggaggcgag aatcttccgc aatttgccgt ggcggatatg aagagccttc cgcacggcga
     7801 agctgacgtc gtgctctgca tgtatgacag catcaattac tgcagcaata cgggagagct
     7861 tgcggcaact ttccgcagct ttcgcaaggc tgttcgcgct ggaggtatcg cgatctttga
     7921 cgttaccacc gaggcaaatt cgctccggta ttttcgagac taccgctatc gcgaacgtca
     7981 cgacgggtac atgatctcga ggcacagtgt gtatcacccc gaaactcgca cacagaccaa
     8041 tgagttcgtt tttgaagggc ccgacttacc ggggcgagtg aaagaaatgc accggcagca
     8101 gattttctcg ctcaaagagg tgctggagag cataccggcg ttcgaatggt cggtgttggg
     8161 ggcatttgac gggtacacac tcgatccgcc ggacgatgat accgaacgaa tccacatcgt
     8221 tatgcgtgct ctgggttgag cgacagggga gtgcatgggc gacaccccgt ttctcgcgtt
     8281 tgatgccgtg actcttcaag ggcgccgggc acccattgtt gaggacgtat ccctgtctct
     8341 tgcggcgggt ggatttggag tccttttcgg ccctaccggc ggcgggaaga cgacgattct
     8401 caggctggct cacctcgagc tccgacctac agtggggcgc gtgaaagtcg gggatatttc
     8461 ctccgaccac ctcaacgccg gcgaagccgc cattgcgcgt cgtgccatcg ggatggtgta
     8521 ccaggattgc cgcctccttt cggacaggtc tgcaagtgag aacatcatac tgccgttgca
     8581 gatcgccgga atggcgaccg ggatggcccg gcgtcgtgca ctcgaggttc tcggtcaggt
     8641 tggattggcg ggaaagcgca gcatttctcc tcgatatttg agcggaggag agctgcagag
     8701 acttgctctt gcccgtgctc tggcgatgga tccccacctt ctcctcgcgg acgaacccac
     8761 aggcaatctt gactcggaca ccgcggaaga gatcatgaca ctgattcgtc gcataaacgc
     8821 gcgcgggacc accgtgcttc ttgccaccca cgatctcaag ctgatcgggg gatacgggta
     8881 cccctgctgg cgagtggagg acggccacgc tgtacaggaa gagccgcggt gacggttgaa
     8941 ggctgcgcgt actgaatcag cgcgtttcga gtggatttcg gttgagcgtg gtcgaggttg
     9001 gtggtgtggc cagccgccca ggctttcagg tgaccccctg cggcaagtcc gaacagctca
     9061 ctttacggac gcttgacgac ttccaccaca tttccggaaa cgtcctccag gtagacgaac
     9121 ctcacgccgt cgcggtgaac gcctacgttc ccgaactgct ctatgtcttc ccggtggatg
     9181 ccgacgtgcc gcggatggtg cccgggaagc acgaaggcga tactgatgtt ggcgaaacga
     9241 accaatgccc acgtgtcgtc ttgatacgcg acctcgcatc tgaaccgcga ggaataccag
     9301 ttcacggcct gtccaatgtc gtccaccacc acggcgacgt ggtccaccgt atcaaaatga
     9361 tcgggcatcg gcacggtctc ccttgcgcat gtcacgcggc cgaggggatg catgcgacag
     9421 gcagttcgag aagcgcgagg acttcgcaat caaacactgc gaacggattt ggctcgggta
     9481 ttgccagatt cagcccctgg tcgcgcatga gcctcgcgac gtcatcactt cttcgggaaa
     9541 gcgctcttcc gatccgaaca acaggaagcg cccgtacgtc gctttttttc tccagcgccg
     9601 ccataaggca gcttgctgcc cgagccacct cctgaatcaa cagacctgac ggagacagcg
     9661 ttccaagagc ggcgcatgcg ctgtctgaac accacaccat ccgctcttga tctgtcattc
     9721 cgcgcgattt caggattccc ggcaatcgtg caaaacactt ttcccgggcc gcaatgtcgt
     9781 accgctcgag ctggtcgtcc gttgtgaacg ccgcaagcag atcgatggct tttgccagcg
     9841 tcaacgctac cgcgacaggt ttgggagaga actcgtcggg cgcgtcccat gactccgcag
     9901 aagcggcgcg gtccatcaac cggcttgcaa gcgacgcgta tcgttcccgc tgaaacaacc
     9961 aggccgcgta cgccagggac aacagcggct cacactggct gacgtgccac gccggctccc
    10021 tggcgaaaac gtcggggtcc cggatgacac tttcggcgtg cgcctccacg gactcggcat
    10081 accggttgtc gcagaggagt ctcgaccgca tatgtgacgc ttcgtcagtc gaccaccaca
    10141 gacgcggtgg tttctcctgc atcgcggaaa cggatttcag aattgtgctc acgtcgaatg
    10201 cagacatatc gtcgttcctg tcattgtact tccgcttggc tgctcttcac ttgattccat
    10261 gcagtttctt gtattccgcc cagttcattt tgagcttcaa aaacgagggg aaatcgggtt
    10321 gcagcaaaaa aggattggat ctgcgttcgt ccccgattgt agaaaaaggc cggacgccgt
    10381 agtcgtgccc gggaaagacg cgggtgttgt caggaagtga cagaagtttc gtgtggaggc
    10441 tttcccattc ctgccgcgct ccttcgccaa agtccgaacc cccgaccttg cccacgaaca
    10501 gggtatcccc cgtgaacact gcgtcctcta caaaatagca tgcatgcccg cgtgtatggc
    10561 ccggtgtgtg gatcaccgtg acggaaagtt cgcccagagg aagtacatcg ccgttatcca
    10621 cacggacatc gcagttccct tcgctcatac gatgcatgac cacctgtgcc ccggtcaatt
    10681 ctttgagtct cgcgttgccg ttcgtgtggt caaaatggtc gtgcgtctcc agcacatatt
    10741 ccacgcggaa accgagtgtc gcggctttct ccgccaccag atccggcgcg aaggaagggt
    10801 ccaccacagc gcatacgcgc cgcgtttcgt cagcaacgag gtaggagaaa ttcctgtcgc
    10861 cgcccgttcg aatttgttcc accagcacgt tgcagctccg gattcgtcag acgattccca
    10921 ttaaaaatgc ccgcgttcgc gtccgtttcc aagaccaggg acaagaaggg cgcaccgggc
    10981 gggttctcga agccccgcat tacgcgatca ggtgcggccg ccacacgaac tggtgaccgg
    11041 ggcgtgctga gtgtaacgcg ggcgatcggt ccatgcgcat tcccac
//
LOCUS       DAOTNG010000070        10995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5605_length_10995_cov_4.348263, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000070 DAOTNG010000000
VERSION     DAOTNG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5605_length_10995_cov_4.348263"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="P5179_04930"
     CDS             <1..1257
                     /locus_tag="P5179_04930"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRS94599.1"
                     /translation="RYGTYPEYGASISAMDLRQIRVSEDDFGLLSFDPGLTNTETTAS
                     AITFIDGERGILRYRGYPIEQLAEKSTFLEVAYLLIHGKLPTFAEFTDWTNKIVQNML
                     IHESIKKFLDGFHSNAHAMGVFVGTLGAKSTFYPAAKHVLDPNQRMRETINIIASVPT
                     IAAYTYRHSCGLPYVYPDPSLSYIYNFLSMLFRMQPGKEEIYHNPVIERALDVLFILH
                     ADHEQNCSTTTMRTVGSALSDPYSAISAAAGALYGQLHGGANQEALEMLSQIGSKENI
                     PAFIKRVKNHETRLMGFGHRVYKNYDPRAKIIKKMADQVLEVTGPDPLIEVARELEGI
                     ALSDEYFIKRKLYPNVDFYSGIIYKALGFPVDMFPVLFALARTAGWLSHWLELMDNEE
                     ERKIVRPRQQYVGEDEREYVPMDDRT"
     gene            1339..1412
                     /locus_tag="P5179_04935"
     tRNA            1339..1412
                     /locus_tag="P5179_04935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1373..1375,aa:Arg,seq:acg)
     gene            1467..1928
                     /gene="tadA"
                     /locus_tag="P5179_04940"
     CDS             1467..1928
                     /gene="tadA"
                     /locus_tag="P5179_04940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRS94600.1"
                     /translation="MDRIPDHETWMRLALAEARAALHEQEVPVGAVVVRDGIVIGRGH
                     NTVETGESALNHAEMLALREATQAVGAWRLNDAVLYVTLEPCMMCAGALMLSRIGGLV
                     FGARDPKFGACGSRIDALAPEMGWNHRVNIISGVLADESAALLREFFSSRR"
     gene            1935..2023
                     /locus_tag="P5179_04945"
     tRNA            1935..2023
                     /locus_tag="P5179_04945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1969..1971,aa:Ser,seq:cga)
     gene            2061..2153
                     /locus_tag="P5179_04950"
     tRNA            2061..2153
                     /locus_tag="P5179_04950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2095..2097,aa:Ser,seq:gga)
     gene            complement(2265..3518)
                     /gene="ahcY"
                     /locus_tag="P5179_04955"
     CDS             complement(2265..3518)
                     /gene="ahcY"
                     /locus_tag="P5179_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRS94601.1"
                     /translation="MASQVKNPALAEQGRRNIEWAERQMGALLKVQERFAKEKPLVGV
                     RVGMALHVTKETAVLVKTLVAGGAEVSITGCNPLSTQDDVAAALALEPHVEVFAHKGE
                     TREEYYDFLNRVIAFKPHVTIDDGCDLVSEIHTKHQGLIPDVICGCEETTTGVIRLAA
                     MEKDCALKYPMIAVNDSDTKHLMDNYYGTGQSTLDGILRASNILISGKVIVVVGYGNC
                     GKGVASRARGLGARVIVTEVNPFRALQATMDGFAVMPMAEAVREGEVFVTVTGNKNVV
                     STEHIRAMKDGAILANSGHFDNEVDVAGLTAMAKAKRTVRPMFDEYDLGGKVVFLAGE
                     GRLVNLAAAEGHPSTVMAMSFCNQAMAVEWGVKNRGKLEAKVYRLPPELDLEVSRLQL
                     EAMGIRIDKLTPEQEAYLNSWQEGT"
     gene            3774..5114
                     /locus_tag="P5179_04960"
     CDS             3774..5114
                     /locus_tag="P5179_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94602.1"
                     /translation="MTPSPITWVPPATFDANAPDLGLPPFPAAAHTVLYDPFPCAANV
                     DEGGDGRYESLRHGTYSHHPAIVLKDRTFIVYWTQHSKDENGPGQRMLACAGQFDEAF
                     EEIDWSRDGALVEIIPPPIPVRRRPWNHDPHIIYPYSLGGLQLINGRLYTSGLIVACH
                     GYTNDVQYHGFQTKPLPASAWSDDLDRERGFIYDLWLDLGLRFVQRWRIQDGVLAADS
                     PMYAQAPLTRRFEVCDGRWKDIAPLLPPYSEALPFDAAPTEMRDDILNGVPVQFARHA
                     KYAPGTSRLTEDGTNGLAHETEFRRRDGKWVVLRDNLERRGHYWAALKEREEDCYPPA
                     VETNLYGGAMPVAGELPSGRVWVICNSFDDYYNAADKSRKVMFITLSDDGITFDRTYE
                     LLHVDREPDGGMYKFGGPQYYKALTVGKNVWVVYSITKEKVGVTKLPVDALERA"
     gene            5175..6041
                     /gene="zupT"
                     /locus_tag="P5179_04965"
     CDS             5175..6041
                     /gene="zupT"
                     /locus_tag="P5179_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRS94603.1"
                     /translation="MDNVLLAFGLTVFAGMATGIGSIIAFTASKTNYRFLSVATGFSA
                     GVMLYVAFVEIFLKGTNALVEAYGGYWGHWANAAAFFGGMLLIGIIDNVIPSAENPHT
                     SPSEDETAPLQERPVTVSSRRSNAGSHTDHKKLLRMGLFTAFAITIHNFPEGLATFLA
                     ALNDPGLGIAIAVAVALHNIPEGVSVSVPIYYATGDRKKAFMYSLLSGVAEPVGAAAG
                     YVAILLLVSDGGAIPPHIMGILFGGVAGIMVYISVDELLPTSRAYGKGHDSLLGMVGG
                     MVVMALSLLLMK"
     gene            6118..7359
                     /locus_tag="P5179_04970"
     CDS             6118..7359
                     /locus_tag="P5179_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRS94604.1"
                     /translation="MTLKKHSPRLGALEILKYIGPGLLVTVGFIDPGNWASNLSAGAD
                     FGYALLWVVSLSTVMLIILQHNVAHLGIVTGFCLSEATSLHVKAPYSTLILSSVMVAS
                     ISTSLAEILGASIALNMIFAIPVPVAAALVTIFVVVMLFTNTYKAVEKGIVALVSIIG
                     LSFLYELSLTTVEWDQVFVSWVTPSFPDGSMLIIMSVLGAVVMPHNLFLHSEIIQTRQ
                     WNLEDGAVIRKQLRFEFLDTLFSMLVGWAINSAMIILAAASFFNAKIPVVDLTQAQKL
                     LAPLLGEHSAFVFAVALLLAGVSSTMTSGMAAGSIFAGMFREPYDVKDSHTRIGIVIS
                     LVCALLVILFISNPFQGLIISQMILSVQLPVTIFLQVYLTSSAKVMGGYRNGPLLIVT
                     LVLLGTIVSALNVLLLISFFR"
     gene            7615..8724
                     /locus_tag="P5179_04975"
     CDS             7615..8724
                     /locus_tag="P5179_04975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS94605.1"
                     /translation="MRREYFVPETLTLWKVATPYPANGNLLYPAGIEHTVVHRAGRDK
                     YRFLHDCAIVAHNGVLFAAWYNCPEGEIVGESLIRGRRSADDGSTWSDVEVIAADRDG
                     KGVFYVPVQFLSTGDDLFAYVSTMTGHDLVVSCEAFKLGASGALSDWVPCGTVAPLFL
                     PNATPVTTDDGTFLMAGRCAERTGQLPTIPAVARSKAPLGDWEVVRLLPEGILPDGRS
                     LKYPETTLIVEGADVTAFVRNDHGFALVFLSTDGGRTWRGPFEQNMPLGAAKMCSGIL
                     STGQRYIVFNTPSDGYRDLLTIAVSRPGEREFSTVWKVAHGFSEVLGCGSEWSYPCAL
                     EHNGMLLIAYTSEKHHCVVARIPVASLIGFPPSRE"
     gene            8833..9102
                     /locus_tag="P5179_04980"
     CDS             8833..9102
                     /locus_tag="P5179_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS94606.1"
                     /translation="MSVLSIGLSKNGLKAGGKVFSDPAVCTCLAEEAGVAVGWISFGK
                     SRDEDAVNCGEVYGLHAHPDHWGHGIGGKELWALRYSTAVPARSN"
     gene            9274..10812
                     /locus_tag="P5179_04985"
     CDS             9274..10812
                     /locus_tag="P5179_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRS94607.1"
                     /translation="MLTHVFSAATHGVDAYIVDVEANLDSQVPSFTVVGLPEGAVKES
                     RDRVIAAVKNTGLYFPLRRITINLAPADIRKEGSAFDLPMAVGILAASEQAVSENLPH
                     AVLVGELSLDGSLRSVPGVLSMAVTAKREGKRMMIVPSDNVQEAALVEGLEVYAAKNL
                     DEALDILANPGGHVPHSLNLAEVFARHVDDTVDMFDVKGQQHVKRALEVAAAGGHNVI
                     MIGPPGSGKTMLARCMPTILPDMTLDEALAVTKIHSVAGQMEPGQALIATRPFRSPHH
                     TISDAGLIGGGTYPKPGEVSLAHHGVLFLDELPEFKRTVLEVLRQPLEDGRVTISRAA
                     TSLDYPAEFLLVASCNPCPCGFFGDASGKCTCTPQMIERYMSKLSGPLLDRIDIHVNV
                     PAVPYKELAADPTGERSAAIRERVNRARDIQAQRFREREGLFCNARMVSRDLRTYCRI
                     DDQSHELLRMAIQKMGLSARAHDRILKVARTIADLDGSNQVTARHVAEAVQYRSLDRQ
                     LWLR"
BASE COUNT         2184 a         3192 c         3313 g         2306 t
ORIGIN      
        1 aggtacggga cgtaccccga atatggcgcg tcaatcagcg ccatggacct gcgccagatc
       61 agagtcagtg aggatgattt cgggctgctc tccttcgacc cggggcttac caacactgaa
      121 acgaccgcca gtgcaatcac gttcattgat ggcgagcggg gcatcctgcg gtaccgcggc
      181 tatccaattg agcaactcgc cgagaaatcg acgttcctgg aagtggcata cctgttgata
      241 cacgggaagc ttccgacgtt cgccgagttt acggactgga cgaacaagat cgttcagaac
      301 atgcttatcc atgagtcaat caagaagttc ctcgacgggt tccacagcaa tgcccacgcc
      361 atgggggtat ttgtgggaac gctcggcgcg aaatcgacgt tctacccggc tgccaaacat
      421 gttctggatc cgaaccagcg catgcgtgaa accattaata tcatcgcatc ggttcctact
      481 atcgccgcgt acacgtaccg ccacagctgc ggattgcctt acgtgtaccc cgatccatcg
      541 ctctcctaca tctacaactt tctgagcatg cttttccgca tgcagccggg taaggaggaa
      601 atctaccaca acccggtgat agaacgagcg ctggacgtgc ttttcatcct gcacgcggat
      661 catgaacaga attgcagcac cactacgatg cgcacggtag gaagtgcact ttcagacccc
      721 tattccgcga tttcggcggc ggcgggcgcg ctttacgggc agcttcacgg cggcgcaaac
      781 caggaagcac tggaaatgct ttcgcagatc gggtcgaagg agaacatccc ggcgttcatc
      841 aagagggtga agaaccacga aacgcgtttg atgggattcg ggcaccgagt ttacaagaac
      901 tacgatccgc gagccaaaat aatcaagaaa atggcagacc aggtgctcga agtgacaggc
      961 ccggatccgc tcatcgaggt tgcaagggag ctggagggaa tcgcgctgag cgacgagtac
     1021 ttcatcaaga ggaagctcta cccgaacgta gatttctact ccggcatcat ttacaaggca
     1081 ctcgggttcc cggttgacat gttccccgtg ctgttcgcgc ttgcccgaac tgcaggatgg
     1141 ctttcccact ggctcgagct gatggacaac gaagaggaac ggaaaatcgt ccgcccgcgg
     1201 cagcaatatg tgggcgagga cgaacgggaa tacgttccca tggatgaccg gacataggac
     1261 ggagtggccc tcgcgggctt gttcctgcgg gatttgggca gtatattaaa aagttcataa
     1321 tcacaaaaac gatcgcgtgc gctcatagct caattggata gagcatctga ctacggatca
     1381 gaaggttggg ggttcgactc cctctgagcg cacttctttt ttctctgccc gtccagcgat
     1441 ttccaccgag gacatgacgc ctgcttctgg atagaattcc ggatcacgaa acctggatgc
     1501 gcctcgcgct tgccgaggca agggcggcgc ttcatgaaca ggaagtgccg gtaggggcag
     1561 tggttgtccg cgacggaatc gtgatcggcc gcggtcacaa taccgttgaa acgggcgaaa
     1621 gcgcgttgaa ccacgcggag atgcttgcgc tgcgcgaagc aacgcaggca gtgggtgcct
     1681 ggcggttgaa tgacgccgtg ctgtacgtca ccctggaacc gtgcatgatg tgcgccggcg
     1741 cgttgatgct ttcgcggatt ggcgggctgg tgttcggcgc gagggatccc aaattcggcg
     1801 catgcggttc ccggattgat gcgctggctc cggagatggg gtggaaccac cgcgtgaaca
     1861 tcatttcggg ggtgcttgcc gacgaaagcg ccgcgctcct gcgcgagttc ttttcgtcac
     1921 ggcgatgatt tcgcggagag gtgcctgagc ggttgaaagg ggcggtctcg aaaaccgttg
     1981 agggttttgc cctcccaggg ttcgaatccc tgcctctccg ccattcttac ccgatctgaa
     2041 gcagtgtatg catctcgtgc ggagaggtgc gggagtggtt taaccggcac gactggaaat
     2101 cgtgtgagcg gtgatgagcc gttccgaggg ttcgaatccc tccctctccg ccagcgtttc
     2161 gttgccttct tcttcgggtt tcccttcgtt cccgaaaaac agaagcgcgg cacccttatc
     2221 gggtcgccgc gctttttccg tgttccggtt ccgggaacgg ttcgtcaggt gccttcctgc
     2281 cacgaattaa ggtaggcctc ctgctccggc gtcagtttgt caatgcgaat gcccattgct
     2341 tcgagttgga gccgcgatac ctccagatcc agttcggggg ggagacggta gacttttgct
     2401 tccagcttgc cccggttctt taccccccat tccactgcca tcgcctgatt gcagaatgac
     2461 atcgccatca cggtggaggg gtgcccttcg gcggcggcaa ggttcaccag ccggccttct
     2521 cccgcaagaa acacgacctt cccgccgagg tcgtattcgt cgaacatcgg gcggaccgtg
     2581 cgtttcgctt tcgccatcgc ggtaaggcct gccacgtcta cctcgttgtc gaaatggccc
     2641 gaattggcaa ggattgcgcc gtctttcatc gctctgatgt gctcggtact cacgacgttc
     2701 ttgttgcccg tgacggtgac aaatacctcg ccctcgcgca ctgcttcggc catcggcatc
     2761 acggcaaatc cgtccatggt agcctgcagc gcccggaagg ggtttacctc ggtgacgata
     2821 acccgtgccc caagcccgcg ggcgcggctg gcaacaccct tgccgcaatt cccgtaccct
     2881 acgacgacaa tgaccttgcc ggagataagg atgttcgatg cgcggaggat gccgtcgaga
     2941 gtggactggc ccgtgccgta gtaattatcc atgagatgct tcgtgtcgct gtcgttgact
     3001 gcgatcatgg ggtatttgag cgcgcaatcc ttttccattg cggcgaggcg tatgacgccg
     3061 gtcgttgtct cctcgcaccc gcagatgacg tccggtatga gcccctgatg ctttgtgtgg
     3121 atttcggaga cgaggtcgca gccgtcatct atggtcacgt gcggcttgaa ggcgatgaca
     3181 cggttgagga aatcgtagta ctcctcccgc gtttcgcctt tgtgggcgaa cacctcgacg
     3241 tgcggttcca gcgccagcgc ggcggccaca tcgtcctgag tcgaaagcgg gttgcagccg
     3301 gtgatagaaa cctcggcgcc acccgcaaca agcgttttca caagcacggc cgtttccttc
     3361 gttacgtgaa gcgccatgcc tacccgcacg ccgacgagcg gcttctcttt cgcgaagcgt
     3421 tcctgcactt tcaagagcgc gcccatctgc cgctccgccc attcaatgtt ccgcctgccc
     3481 tgctcggcca gcgcggggtt tttcacctga gacgccattg ggtatccttt caacagccta
     3541 agccgcgcga accatctgct gcgcgcgcga tcaagaacag acacaaggtt ttaagtttat
     3601 gagtttttcg ctggttttgc caaggtttga cgagtggggg gtggccatca gatccgcgga
     3661 agcggccgtg cacgagaaac gaagcttccg ctggataccg cacggcagat aacgcgaccc
     3721 gcgcacatgg gttcaggtac ccgaaaatcc gattcccgca aaggaaccaa cacatgacac
     3781 cgtcgcctat cacatgggtt ccaccggcaa ccttcgacgc aaacgcgcct gacctcggtc
     3841 tgccaccttt ccccgcagca gcacacaccg tcctgtacga ccccttcccc tgcgccgcga
     3901 acgtggacga gggcggcgac gggcggtacg aaagtttgcg ccacggaacc tacagccatc
     3961 atccggcaat cgtgctcaaa gaccgcacct tcatcgtgta ctggacacag cacagcaagg
     4021 acgagaacgg accaggccaa cgcatgctcg catgcgccgg gcagtttgac gaagcattcg
     4081 aggagatcga ctggagccgg gatggcgcgc tggtggagat catcccgccg ccgattccgg
     4141 tccgcaggcg accgtggaac cacgacccgc acatcatcta cccttattcg ctcggcggcc
     4201 tgcagcttat caacggaagg ctgtacacca gcgggttgat agttgcctgc catggctaca
     4261 ccaatgacgt gcaataccac gggtttcaga ccaaaccgct ccctgcctcg gcatggagcg
     4321 acgacctcga ccgcgaacgg gggttcatct acgacctgtg gctggatctc ggactgcgtt
     4381 tcgtccagcg gtggagaatt caggacggcg ttctcgcagc cgattccccg atgtacgccc
     4441 aggcgcccct cacgaggcgg ttcgaggtgt gcgatggaag gtggaaggat atcgcgccgt
     4501 tgttaccgcc gtattcggag gcgttaccgt ttgatgccgc cccgaccgag atgcgagatg
     4561 acatcctgaa cggcgtgcca gtccagtttg cgcgccatgc gaaatatgca cccggcacga
     4621 gcaggctgac ggaggacggc acgaacggtc tcgctcacga gacggaattc cgccgccgcg
     4681 acgggaagtg ggtggtgctg cgggataacc tcgagcgccg gggccactac tgggctgcgc
     4741 tcaaagagcg ggaagaggac tgttacccac cggcagtgga gacgaacctt tacggcggag
     4801 cgatgccggt ggcgggagaa cttccgagcg ggcgggtgtg ggtcatctgc aacagcttcg
     4861 atgactacta caacgccgct gacaagagcc ggaaggtcat gttcatcacg ctctcggatg
     4921 acggaatcac cttcgacagg acgtacgaac tccttcacgt ggatcgtgag ccagacgggg
     4981 gtatgtacaa gttcggcggt ccgcagtact acaaagccct gacggtgggc aagaacgtct
     5041 gggtggtgta cagcatcacg aaggaaaagg tcggtgtgac gaaattgccg gtggacgcgc
     5101 tggaaagagc gtgaacggta gccgattcga ggaacgttcg gggcgacggt caagatcatc
     5161 agggaaaaca gtgtatggac aatgtgcttc tcgcgttcgg gctcacagtg ttcgccggca
     5221 tggcaacagg catcggcagt attatcgcgt tcaccgccag caagacgaac taccgcttcc
     5281 tctccgtagc caccggtttt tcggccggcg tcatgctcta cgtagcgttc gtggagatct
     5341 ttctgaaggg cacgaacgcg ctggtcgagg cctatggcgg ctactggggt cactgggcca
     5401 atgccgcggc gtttttcggc ggcatgctgc tcatcgggat catcgacaat gtgatcccgt
     5461 cggcggagaa cccgcacacc tcgccatctg aagacgagac ggcgccgctg caggaacgcc
     5521 ctgtgacggt atcgagccgg agaagcaacg ccggttccca taccgaccac aaaaaactcc
     5581 ttcggatggg tcttttcacc gctttcgcca tcactattca caacttccct gaggggctgg
     5641 cgacgttcct ggccgccctg aacgatccgg ggctcggtat tgccatcgcg gttgcagtgg
     5701 cgctgcacaa catcccggaa ggagtcagcg tctccgttcc catttactac gcaaccggag
     5761 accgaaaaaa ggccttcatg tactccctgt tgagcggagt agctgagccg gtgggcgcgg
     5821 cggcgggcta tgtggctatt ctgttgctcg tgagtgatgg cggcgccata cctcctcata
     5881 taatggggat cctgttcggc ggggtggccg gtatcatggt gtacatcagc gtggacgagt
     5941 tacttcccac gagccgcgcg tatggcaaag ggcacgacag ccttctcgga atggtcgggg
     6001 gaatggtggt tatggcgctt agcctgttac tgatgaaatg ataaaccggg cgaaaaaccg
     6061 gcacgttttc ggtttttgtg ctgcttttca aggtatttcc gagacacgga tatccaaatg
     6121 actttgaaga aacactcacc gcgtctgggt gcgctggaga tcctgaagta catcggcccc
     6181 ggtttgctcg tcaccgtggg gttcatcgac cccgggaact gggcgtcgaa tctttccgcc
     6241 ggggcggact tcgggtatgc attgctgtgg gtggtctcgt tatcaacggt gatgctcata
     6301 atcctccagc acaacgtcgc ccatttgggt atcgttacgg ggttctgttt gtctgaagcc
     6361 actagcttgc acgtgaaggc gccctactcc acgctgatcc tgtcctccgt gatggttgcg
     6421 tcgatatcca cctccctggc agaaatcctc ggggcaagca tcgcactgaa catgatcttt
     6481 gccatccctg ttccggtcgc tgcagccctg gtaacgatat ttgtcgtcgt gatgctgttc
     6541 acgaacacct ataaggctgt tgaaaaaggc atcgttgcac tggtatcgat tatcggtctt
     6601 tcgttcctct acgaactgtc attgaccacc gtggaatggg atcaggtttt cgtgagctgg
     6661 gtcacaccct cattcccgga cggctcaatg ctgatcatca tgagtgtgct gggtgccgtc
     6721 gtaatgcccc acaatctgtt cctgcactca gagataatcc agactcgtca gtggaacctg
     6781 gaagatggtg cggtaattcg caagcagctt cgattcgaat tccttgatac gttgttttca
     6841 atgcttgtcg gctgggcgat aaacagcgcg atgatcatcc tcgcagccgc gtccttcttc
     6901 aatgcgaaga ttcctgtcgt ggaccttact caggctcaaa agctgctggc gccgctcctg
     6961 ggagaacatt cagcgtttgt gttcgccgtt gcattgttgc tggcaggcgt ctcttccacc
     7021 atgacatcgg gcatggctgc gggaagcatt tttgcgggga tgttccggga gccttacgac
     7081 gtcaaggact cgcacacaag aatcgggata gtcatttcgc tggtgtgcgc attgctcgtg
     7141 atactgttca tcagcaatcc gtttcaaggg ttgataatct ctcagatgat tctgagcgtg
     7201 caacttccgg taacaatctt cctgcaggtc tatctcacct catccgcaaa ggtgatgggc
     7261 gggtacagaa acggccctct gctcattgtc actctcgttc ttctcgggac gatagtctcg
     7321 gcgctgaatg ttctgcttct catcagcttt ttccgttgaa ccgctgaaag gctgaccgga
     7381 ctgtggcgtt gctttcgtta ccgttccgtt ccctctctgc actcggcggg aggcaggacg
     7441 ggcacggtcc cacggcccaa acgggaaaaa tccgccgttt ccacgagctg gcgagtcacc
     7501 atcgggagcg ttccacatgg attcccgcct gcgtgggaat gagggatcaa cgcgggggag
     7561 gggcgtcatt cttggagaag cgaagaaact gacacgccac gcaggaacca cggcatgagg
     7621 agggaatact tcgtgcctga gactctcacc ttgtggaagg tggcaacgcc ctacccggcg
     7681 aacggcaacc ttctttaccc cgccggaatc gagcacactg tcgtgcaccg cgcaggcaga
     7741 gacaaataca gatttctcca cgactgcgca atcgtggcgc acaatggcgt cctttttgcc
     7801 gcatggtaca actgccccga aggtgagatc gtcggcgagt cactcatccg tggccggcga
     7861 tcggctgatg acgggagcac gtggtccgac gtggaggtta ttgcggcgga ccgagatggc
     7921 aaaggcgtat tttacgtccc cgtgcaattc ctgtccaccg gggatgacct gttcgcgtac
     7981 gtttcgacga tgacgggtca cgacctggtg gtctcgtgcg aagcgttcaa actgggcgcc
     8041 tcgggggcgt tgtcagactg ggttccctgc ggcacggtcg caccgctttt cctgccaaac
     8101 gcgaccccgg taacgacaga cgacggaaca ttcctcatgg caggacggtg cgccgaacgg
     8161 acgggccagt tgccgacaat tcccgccgtt gcgcggagca aggcgccgct cggtgattgg
     8221 gaggttgtaa ggctcctgcc cgaaggcata cttccggatg gtcggtcgtt gaaatacccc
     8281 gaaacgacgt tgatcgtgga aggggcggac gtgactgcct tcgtccggaa tgaccacggt
     8341 tttgcgctcg tgttcctgag cactgacggc gggaggacgt ggcgcgggcc gtttgagcag
     8401 aacatgcccc ttggcgcggc gaaaatgtgt tcgggcatac tgagcaccgg gcagcggtac
     8461 atcgtcttca acacgccctc ggacgggtat cgcgatcttc tcacgatcgc ggtcagcagg
     8521 ccgggtgagc gcgagttttc cacggtctgg aaggtcgccc acgggttttc tgaagtgctg
     8581 ggctgtggtt ccgagtggtc gtacccctgc gcgttggagc acaacgggat gctgctcatc
     8641 gcgtatacct cagagaagca ccattgtgtg gtcgcgagga taccggttgc gtccttgatt
     8701 ggattcccgc cttcgcggga atgacgcgcc gggggcaaat cccgcggagg gagagcgcag
     8761 aaatgatgtg acgggccacc ctcgaggacg atgttcgttc ccggcaggag gcttacaaag
     8821 ggatcgttcc ggatgagcgt gttgagcatc ggactgtcga aaaacggtct gaaagctggc
     8881 ggcaaagtgt tttccgatcc cgcagtatgc acgtgcctgg cggaagaagc cggggtagcg
     8941 gtcggttgga tttcgttcgg aaaaagcagg gacgaggacg cggtcaactg cggagaggtg
     9001 tatggccttc atgcgcatcc cgaccactgg ggtcatggca tcggcggcaa ggagttgtgg
     9061 gcgcttcggt attcaacggc agtccctgcc cgaagtaact gacgagtccg aatccatcat
     9121 tttcccccgt tggcatcctc ccggccaatt cgccgcgtga tctgctgtcg tttcccaacc
     9181 cccactacgc gtcacacaca caccactcgt ataatgaatg ttccggtaga gactgacaat
     9241 ttcggttcta cataagccga ggaagtacgt ttcatgttga ctcacgtgtt ttccgcagca
     9301 acacacggcg tcgatgcgta catcgttgac gtggaggcga atctcgactc gcaggtgccc
     9361 tcgttcaccg tggtaggact cccggaaggc gcggtgaaag agagccggga tcgtgttatc
     9421 gccgcggtga aaaacacggg cctctatttc ccactccgcc gcatcaccat caaccttgct
     9481 cccgctgaca tccgcaaaga agggagtgcc ttcgaccttc ccatggccgt cggaatcctc
     9541 gccgcgagcg agcaggcggt aagcgaaaac ctcccccacg ccgtacttgt tggcgagctt
     9601 tccctcgacg gctcactgcg atcggttccg ggggtgcttt ccatggcggt gaccgcgaag
     9661 cgtgaaggca agcgcatgat gatcgttcct tccgacaatg ttcaggaagc ggctctggtg
     9721 gaagggctcg aggtgtatgc cgcaaagaac ctcgacgagg cgctggatat cctggcaaat
     9781 ccgggggggc acgttcccca ctccctgaac ctcgcggagg tgtttgcccg tcacgtggat
     9841 gacacggtgg acatgtttga cgtgaagggc cagcagcacg tcaagcgcgc gctcgaggtc
     9901 gccgccgccg gggggcataa cgtcatcatg attggccctc ccggctccgg gaagaccatg
     9961 ctcgcccgat gcatgccgac gatcctcccc gacatgacgc tggacgaagc gctcgccgtc
    10021 acgaaaatcc attccgtggc aggacagatg gaaccagggc aggcactcat cgccacacgg
    10081 ccgttccgat ctccccacca tacaatctcc gacgcggggt tgattggagg cgggacctac
    10141 ccaaagccgg gagaggtgag tctggcacat cacggagtgc tttttctgga cgagttgccg
    10201 gagttcaagc gcaccgtgct ggaggtgctg cgccaaccgc ttgaggacgg ccgcgtcacc
    10261 atttcccgcg cggccacttc gctcgactat cccgccgagt tccttctcgt cgcgtcgtgc
    10321 aatccctgcc cgtgcgggtt tttcggcgac gccagcggaa aatgcacctg cacgccgcag
    10381 atgatcgaac gctacatgag caagctctca ggcccgttgc ttgaccgcat tgacatccac
    10441 gtgaatgtgc cggctgtgcc gtacaaagag cttgccgcgg acccgaccgg ggagcgatca
    10501 gccgcgatcc gggaacgggt caaccgggcg cgggatattc aggcgcagcg cttccgtgaa
    10561 cgggagggac tcttttgcaa cgcccgcatg gtgagccgcg accttcgcac gtattgccgt
    10621 attgacgatc agagccacga gctgttgcgt atggcaatcc agaaaatggg gctttccgca
    10681 cgcgcacacg accgcatcct gaaggttgcc cgcaccattg ccgacctcga cgggtcgaat
    10741 caggtgacgg cgcgccacgt cgcggaggca gtgcagtacc gaagtctcga ccgccagttg
    10801 tggttgaggt agggcgctct gcgtgccggg ccacctagcc gatgtcggga gtgccgacat
    10861 atgcgttgcg caactgcgcg ctcctctctg gattcccgcc tgcgcgggaa tgacgaacgt
    10921 tgcgttcggg cacagggggc cgggtcgccc ggcccggtgg ctggtcgtaa catgggcatc
    10981 ttgcccatgc acgta
//
LOCUS       DAOTNG010000071        10931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5644_length_10931_cov_5.516918, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000071 DAOTNG010000000
VERSION     DAOTNG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5644_length_10931_cov_5.516918"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="P5179_04990"
     CDS             <1..865
                     /locus_tag="P5179_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94608.1"
                     /translation="ACDRCSPWGCNGFLRLSHDFTELAREYFPNIKTVLSTWMFDTPP
                     EGEWEGLTEALKDGNNWVDYILADAHEDFPRYPLDVGVPGNLPLVNFPEISMWGNWPW
                     GGVGANPLPARFQRLWDQVKHIVRGGFPYSEGIYEDMNKAIVAQFYWTPERSARDTLR
                     EYIAYEFGDKAIEETVALVEALEMAATRSYTKQLVDVGLVRTARELADKVHEKMPAWA
                     RTGWRWEILRLRAILDHERFAGEGFATSEAERALERLIEIYHCEMETDDPYHHRVRPP
                     LARAVSRRGNL"
     gene            complement(954..1379)
                     /gene="rplS"
                     /locus_tag="P5179_04995"
     CDS             complement(954..1379)
                     /gene="rplS"
                     /locus_tag="P5179_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRS94609.1"
                     /translation="MDLVDLVDRKHRREEERNENGEVTFAIPEFKTGDLVDVHVRIVE
                     GDNERIQVFRGTVIQRHGGSSSNASFTVRKVTAGIGIERIFPLHSPRLAKIEVVRRGR
                     VRRSRIFYLRDLRGKAARVTEKRDLRTAEQKAAAVETAQ"
     gene            complement(1541..2212)
                     /gene="trmD"
                     /locus_tag="P5179_05000"
     CDS             complement(1541..2212)
                     /gene="trmD"
                     /locus_tag="P5179_05000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRS94610.1"
                     /translation="MEIDVITLFPELFVSPLAVSILGRAQSKGLVAVRPHDLRQWADD
                     ARGTVDDTPFGGGGGMVLMPEPLKRGVEAVKKDHGDGAVILLAPDGERLTQHIANELS
                     LEQHLILICGHYRGVDERFRARYVDREVSIGDYVLTGGELPALVLVDAVVRLIPGVLG
                     NFESAMEDSFQDGMLDCPWYTRPQVFEGEAVPQVLVSGNHAEIRKWRRQQALERTRAR
                     RPDMV"
     gene            complement(2235..2750)
                     /gene="rimM"
                     /locus_tag="P5179_05005"
     CDS             complement(2235..2750)
                     /gene="rimM"
                     /locus_tag="P5179_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRS94611.1"
                     /translation="MRPQRVPIGRILRPHGVRGELKVELLSDEATRFDSVGKVTVTRN
                     GVDLGEFEVTGWRRLGRAIGLKLVGIDTPEAALPLRNALLEAPALPAESLPENTYFVY
                     DLVGLHVVSEDLEHLGEVVDVVQLPSGGLLVIKAGGTEHLVPLVQDFVKRVDLEEKAV
                     TLHLVPGLLDL"
     gene            complement(2770..3030)
                     /gene="rpsP"
                     /locus_tag="P5179_05010"
     CDS             complement(2770..3030)
                     /gene="rpsP"
                     /locus_tag="P5179_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRS94612.1"
                     /translation="MPATMRLKRIGAKKAPSYRVIITDRKNRRDAEAVEEVGFYSPAQ
                     NPPIVTFKKERVEYWLSVGAVPSETVGNLLKNAGISRPKASA"
     gene            complement(3083..4435)
                     /gene="ffh"
                     /locus_tag="P5179_05015"
     CDS             complement(3083..4435)
                     /gene="ffh"
                     /locus_tag="P5179_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRS94613.1"
                     /translation="MLESLSSALDDIFRRLRGRGALSPGNVKDALGEVRKALLAADVN
                     FHVAKEFVKRVEHLAVGQEVLRSISPGQQVVKIVYDELVRLMGSSASTLAIDGAMPAV
                     VMLAGLQGSGKTTACGKLALFLKGKGRHPHLIAADVYRPAAIDQLEQIGKQAGVPVFR
                     SPEGTNPVNIARQGIAAARKAGDDVVILDTAGRLNIDEEMMRELEEISAEVKPQETLL
                     VVDAMTGQIAAEVARDFGRRLDLTGVILSKMDGDARGGAALSVREVSGKPIKFISTGE
                     TLDALDVFHPDRMANRILGMGDIVSLVERAQAAVNQEEARELEKKLRTRAFTLDDFLG
                     QLQQVKKMGPLDQLLGMLPGMNRELLGAKVDDKALGRIEAMIHSMTREERERPEIING
                     GRRRRISTGSGTTVQELNRLLKQFDVMRKMLSKHRPGSKATAAAMSQKLANVSPRRFR
                     "
     gene            4603..4680
                     /locus_tag="P5179_05020"
     tRNA            4603..4680
                     /locus_tag="P5179_05020"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4638..4640,aa:Asp,seq:gtc)
     gene            complement(4720..4950)
                     /locus_tag="P5179_05025"
     CDS             complement(4720..4950)
                     /locus_tag="P5179_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94614.1"
                     /translation="MKQHQIMLADLPAWTESLGCKTEDLVEMLGWLVDTKSELLNDFS
                     LDMHLVDAIVQCRLRREGRFDRREWVGEDAPE"
     gene            5147..5545
                     /locus_tag="P5179_05030"
     CDS             5147..5545
                     /locus_tag="P5179_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS94615.1"
                     /translation="MPGPVSLHHVAIVTTKFDESLKFYRDVLGLGLVHQWKGGDRRLC
                     LLAVGNARIELIEKLTPPDGAGQAAEPPIAHLTFRVDDVDTWTETVRAYGCEITTEPK
                     DVVLGDAPARLSFFVGPNGETLEFFSSKAV"
     gene            5585..6106
                     /locus_tag="P5179_05035"
     CDS             5585..6106
                     /locus_tag="P5179_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94616.1"
                     /translation="MNSQQRREYFRQHLKSVLAQENPFLRHITFRYPPTESEWGQFPT
                     EITVNGQHPGILELRVKTTAATPEVQVRFRAKGFSFSPDEDDPSYSVFSAVADKAGAD
                     ELADFVEWAFLVALDSPDDYDPLIRTPVSEQGPSGAPAKPGKGCSSGCRNIALALLWL
                     LLAIFALRFLFGG"
     gene            6131..7348
                     /locus_tag="P5179_05040"
     CDS             6131..7348
                     /locus_tag="P5179_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRS94617.1"
                     /translation="MIELEEIQRHLAATSVDGWLLYDFQGINPVAARVAGLGEKMLTR
                     RWFCLIPRQGNPEWLVNQIERQQFADVEGVVRTFSTWQQLVSGLSSLLQGRSRVAMEY
                     SPGGTIPYVSRVDAGTLEIVRNTGCAVVSSADLVQWCEARLSASALATHLYAAKHLET
                     AKESAFEFIFRQMEAGKRISEYDIQQEIIRYFDLHGLVTSSPPIVAAAQNTSDPHYQP
                     TSSRAKLLEEGDLVLVDLWAKTRDPGAVYADITWMAHVSDSVPDELARVFAAVVRARD
                     AAIDFLKRSVARGDIVLGYEVDDVARGSVTKDGYGDRFIHRTGHNIGTEDHGRGVNFD
                     NYETHDDRRVIPNITCSIEPGLYFGFFGMRTEVNVHIGARSVEVSTVPMQRQIIPLQR
                     HFLREATSPSEGL"
     gene            7348..8403
                     /locus_tag="P5179_05045"
     CDS             7348..8403
                     /locus_tag="P5179_05045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS94618.1"
                     /translation="MLDYLQEQTARINQQLEAVLPRSITAEWVEAVLGRSTYSVDEET
                     LTAALSDPVWDFLDRGGKRWRPSLMLTCCEAVCGSIEPARPLVPLIELIHNGTLVHDD
                     LEDNSDLRRGKPCLHKSFGIDIATNVGSAMYFIPLVILYGRRAHYSDAQCLYIYDLVA
                     QELVRCHCGQAIDIAWHNGRRDDITEDEYLQMCAYKTGVLARLAARLGAYVGGATPQQ
                     VEELGRFGETIGVAFQIQDDILNLVGERFAEGKGRGEDIHEGKRTLLALHALSHASSS
                     QAARLREILAAHPTEQQVLDEAISIIAACGSIDYARRKAVALVEASWEKVATLVQESD
                     AKKRLRAFADFVVQRDL"
     gene            8489..9523
                     /locus_tag="P5179_05050"
     CDS             8489..9523
                     /locus_tag="P5179_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94619.1"
                     /translation="MRRTTPPFRVGIIGCGGISHAHALGFGNLPALFEVRAVCDSNEK
                     AARKLAEPFGEPRICSDWREVIADPEIDAVDILLPHHLHALVGVSALRAGKHVCLEKP
                     LAITLSEGIELVLEAEASNAVFMVAFNERHRWSVRTIKKIVDEGRIGDVYMVRTDHNQ
                     NPRFAANAWYRNGQLAGGGALIGSGIHMLDLLQWFCGTATSVFATGVKLPEVLETETA
                     ASVIVEFAPRKEGCTDCIGTLDISWAAPNHPWYQFLVVYGTRGTVSTLRGDVVSLRTA
                     GEQEEFHAPEGDTWTDSFREELRHWGECIAQGTRPLTDAREAFRSLEICMAAYRSMDT
                     HTAVRIPLPE"
     gene            9528..10418
                     /locus_tag="P5179_05055"
     CDS             9528..10418
                     /locus_tag="P5179_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRS94620.1"
                     /translation="MRVACIINPRAGRRPHAEHVIAGLRSVASMHGWELRTVTTTGPG
                     EATQIARSLVPASDIIVAAGGDGTVHEVSAALVGTQVALAVIPCGSGNGFARSFKIPL
                     DPLRAIRSLVDSEQISVDVGTVEGRHFFATAGIGLDAEISRRFAAGNHRRGIWPYFRH
                     GFAAWVAYCPQKVTIQSGNMSLSAKPLVLAVANTEQYGGGAKIAPGASPFDGLLNITI
                     VETSSFLRLLIAFPRLFTGSLDRSPLVRRMTATEFTIHRQDPGPYHADGEPFDGPAVL
                     HFSVVPHALRLLVPRGKYPG"
     gene            10524..>10931
                     /locus_tag="P5179_05060"
     CDS             10524..>10931
                     /locus_tag="P5179_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRS94621.1"
                     /translation="MLKRLLETDRNDLAGFVARVFLGIVMFPHGAQKMLGWFGGHGFS
                     ATMGFFGSMGIPPVFAFLAIFAEFFGALGVIFGFLTRVAAFGIGMVMLVAMTAVHLPN
                     GFFAQQNGIEFTLILFGVGLAVMIKGGGRWSLDE"
BASE COUNT         2227 a         3257 c         3167 g         2280 t
ORIGIN      
        1 cgcgtgcgac cggtgctcgc cctgggggtg caacgggttt ctccgccttt cccacgattt
       61 caccgaactg gcgcgcgagt acttcccgaa catcaagacc gttctttcga cgtggatgtt
      121 tgatacacca ccggaaggag aatgggaggg cctgacggaa gccctgaagg acggcaacaa
      181 ctgggttgat tacatcctcg cagatgcgca cgaggacttt ccccgctacc cgctcgacgt
      241 cggtgtgccc ggcaatctac ctctcgtcaa cttcccggaa atcagcatgt ggggcaactg
      301 gccgtggggc ggcgttggcg ccaacccgct ccccgcgcgt tttcagcggc tctgggatca
      361 ggtgaagcac attgtgaggg gtggatttcc gtacagcgaa ggcatctacg aagacatgaa
      421 caaagcgata gtggcgcagt tctactggac gccagaaagg agtgcacgcg acactctcag
      481 agagtacatc gcctacgaat tcggagacaa agcaattgaa gagacagttg cactcgtgga
      541 agcgctggaa atggctgcca cacgctcata caccaagcag ctggtggatg tcgggttggt
      601 gagaactgca cgggaactcg cagacaaagt gcatgagaaa atgcccgcat gggcgcggac
      661 aggctggcgg tgggagattc tgcgtctgcg cgcgattctc gaccatgagc gattcgcggg
      721 cgaagggttc gcaacttccg aggccgaacg tgcgttggag cgtctgatcg agatttacca
      781 ctgcgagatg gaaacggatg atccgtacca ccaccgtgtg aggccgccat tggcgagggc
      841 ggtgtcgcgc cgggggaacc tctgaatctg tgcgttccga aaggtgcaga cggatgacgg
      901 cgcagaggag agacacgctc ctctgcgccg ttccatgtca attccccgaa gtcttactgc
      961 gccgtctcga ctgccgcagc tttctgctcg gcggtgcgca ggtcccgttt ctcggttacc
     1021 cgtgccgcct tcccacgcag atctctcaga tagaaaatac gggaacgtct cacgcggccc
     1081 ctgcggacta cttcgatctt cgcgaggcgg ggcgaatgga gcgggaaaat ccgttcgatg
     1141 ccgatgcctg cggtcacttt gcgtacggtg aacgaagcat tgctggatga tcccccgtgc
     1201 cgctgaatca cggttccgcg gaacacctgt atgcgctcgt tgtcaccttc cacgattcga
     1261 acatgcacgt ccaccagatc acccgtcttg aattccggaa tcgcgaaggt gacttcgccg
     1321 ttctcgttcc gttcctcttc ccgccggtgt ttgcggtcta ccaggtcaac cagatccatg
     1381 tttcctccat tcggcacgta gtgcctgttt tgtcccaccg gcactgagtg ccctgtagcg
     1441 cgcagttcac cgcgcattca aatacgtttt gccgttcggg agacctgcac ccgactgagg
     1501 ggagcccgat gctcgcgttc gaggggtgca ggctttcgat ctacaccatg tcgggcctgc
     1561 gagcccttgt cctttcaagc gcctgctgtc ttctccactt ccggatttcc gcgtggtttc
     1621 cactcacaag aacctgcggt actgcctcgc cttcgaacac ctgaggacgt gtataccagg
     1681 gacaatccag catgccatcc tgaaacgaat cctccatggc ggactcgaaa ttgcccagca
     1741 cacccggaat cagccgcacg acggcgtcca ccagcacgag agccggaagc tcccctccgg
     1801 taaggacgta atcgccgata gatacttccc tgtccacgta cctcgctcga aaccgttcgt
     1861 ccacgccgcg ataatggccg caaatcagga tgagatgttg ctccagcgac aattcgttcg
     1921 cgatatgctg cgtcaggcgt tctccgtctg gagcaagaag aatgaccgca ccgtcgccat
     1981 ggtctttttt gacagcctct acgccgcgtt tcaacggctc cggcatcaaa accatgccgc
     2041 cgccaccacc aaatggcgtg tcatctactg ttccacgcgc atcatccgcc cactggcgaa
     2101 gatcgtgcgg gcgaacggcg accagaccct tgctctgcgc gcgcccgaga atgctgacgg
     2161 caagcggcga aacgaaaagc tcaggaaaaa gcgttattac gtcgatttcc acgtccgtcg
     2221 cctccgccag gctttcacag gtccagaagc cccggcacga ggtggagcgt cactgccttt
     2281 tcttcaagat cgacgcgttt cacgaaatcc tgcacgagcg ggaccagatg ctcagttcca
     2341 ccggccttta ttacgagcag ccctcctgac ggcaactgaa ccacgtcgac aacctctccg
     2401 agatgctcaa ggtcctcgga cacgacgtgc aaacccacaa gatcgtacac gaagtacgtg
     2461 ttttcgggca gcgattcggc gggaagcgcg ggcgcttcca gcaacgcgtt gcgcaacggc
     2521 aatgccgctt ccggtgtgtc gatgcccacc agcttcaggc cgatcgccct gcccagccga
     2581 cgccacccgg taacctcgaa ctcgccgaga tcgactccat tcctcgtcac cgtcactttg
     2641 ccgacggaat cgaaccgggt cgcctcatcg gaaagcaact ccaccttgag ttcgccccgc
     2701 actccgtgcg ggcgaagaat acgccctatc ggaacccgct gtgggcgcat tcgcggacct
     2761 ccggcgccgt caggcagatg ctttgggccg tgaaatgcca gcgtttttga ggagattccc
     2821 cacggtttcg ctgggaaccg caccgacgct cagccagtac tccacacgct ctttcttgaa
     2881 cgtgacgatg ggcggattct gcgcggggct gtagaaaccg acctcttcca ctgcttccgc
     2941 atcacgccga tttttccggt cggtaatgat cacccggtag ctgggagctt tctttgcgcc
     3001 gattcttttc agacgcattg ttgctggcac gcgtaactcc tctgaaagta gtgcacgaaa
     3061 tccgcaacga cgccggaata tcctaccgga atcgtctcgg agagacattt gcgagcttct
     3121 gagacatcgc cgctgccgtg gccttgcttc ccggtcgatg cttcgacagc attttccgca
     3181 tgacgtcaaa ctgcttgaga aggcgattca gttcctgaac tgtggttccc gaccccgttg
     3241 aaatgcgtcg gcgacggcct ccattgatga tctccgggcg ttcccgttct tccctcgtca
     3301 tcgaatggat catggcttca atgcgaccaa gagccttgtc gtcaactttc gcgccgagca
     3361 gttcacgatt catgccgggc aacatgccga gaagttggtc caaaggcccc atctttttta
     3421 cctgctgcaa ctgacccagg aaatcatcca acgtgaaggc gcgggtccgc agtttctttt
     3481 ccagttcccg tgcttcttcc tggttcactg ctgcctgagc gcgttcgaca agcgagacga
     3541 tgtcgcccat tcccagtata cgattcgcca tcctgtcggg atgaaatacg tccagcgcgt
     3601 caagggtttc accggtggaa atgaacttga tcggctttcc ggaaacctct ctcacgctga
     3661 gcgctgcgcc accgcgtgcg tcgccgtcca tcttcgaaag gataacgccg gtcagatcca
     3721 gacgccggcc gaaatcacgt gccacttcgg cagcgatctg tccggtcatc gcatccacga
     3781 cgagaagcgt ctcctggggc ttgacctcgg cggaaatctc ttccagctcc cgcatcatct
     3841 cttcgtctat attcaaacgc cccgcagtgt caagaatcac gacatcgtca ccggcttttc
     3901 gcgcggcggc tataccctga cgggcgatat tcacggggtt tgtgccctct ggcgaacgaa
     3961 aaaccggaac gcctgcctgc ttgccgatct gctcgagctg atcgattgcc gcagggcggt
     4021 acacgtccgc ggcaataagg tggggatggc ggcccttccc tttcaggaat aaagccagct
     4081 tcccgcaggc agttgtcttc cccgaaccct gcaaccctgc cagcatgacc acggcaggca
     4141 tggctccgtc aatcgcaagg gtcgacgcgg aggatcccat caacctcacc agctcgtcgt
     4201 acacaatctt tacgacctgt tgacccgggc tgatgctgcg aaggacttcc tgccccaccg
     4261 cgaggtgttc cacccgtttg acgaactctt tcgcaacgtg gaaattgaca tccgctgcga
     4321 gcaaggcctt gcgaacctcg cccagtgcgt ctttcacatt gcccggcgaa agggcacctc
     4381 ttccgcgcag tctgcggaag atgtcgtcaa gggcggagga gaggctttca agcatggcaa
     4441 atgccggtaa gtgtgtcgcg acaaaacaga cttataaaga tagtcagcgc aattcatcat
     4501 tgcaaatgat caacgggctt ccggttgttc gctgcaacga acgacatcga agcactattg
     4561 acagcatccg ggcagttccg tattctttgt attcctgttc gcggggtagt agctcagctt
     4621 ggttagagtg ccgggctgtc aatccggagg tcgcgggttc gagccccgtc taccccgcca
     4681 tgtgtttctt gacccttgca gcgcaactgc gagggttttt cattccggtg cgtcctctcc
     4741 cacccattcg cgccggtcga atcgcccctc gcgtcgcaac cgacactgaa caatcgcatc
     4801 cacaaggtgc atgtcaaggc tgaaatcgtt cagcaactcg gattttgtgt ccaccagcca
     4861 tcccagcatc tccaccaggt cttcggtctt gcacccgagg gattccgtcc acgcgggaag
     4921 atcggccagc atgatctggt gttgcttcat acggcgcaaa cgtcgcaaat gcgcggcgat
     4981 tttctgtaac tgaatgtcgt ctggtggcac gccccattca gaagccaccg gccgcgacgc
     5041 gtgttccccc ctagactcag aacctccggg gccggatatt tgaaaaaaaa ggcgccagtc
     5101 caaatgccgc ctgcaccagt tccgcaaatc gccagaggag aaccgcatgc caggtcctgt
     5161 ttccctgcac cacgttgcaa tcgtgacgac aaagtttgac gaaagcctca agttttaccg
     5221 cgatgtgctg ggtttgggcc tcgtgcacca gtggaagggt ggcgaccgtc gcctgtgcct
     5281 tctagccgtc ggaaacgcgc gcatcgagct gattgagaag ctcacacctc ccgatggcgc
     5341 gggtcaagct gcagaaccac cgattgcgca ccttacgttc agagtggatg acgtggatac
     5401 gtggacggag accgtgcggg cgtacggatg cgagattact accgagccaa aagacgtcgt
     5461 tctcggcgac gctcctgcgc gactttcgtt ttttgtcgga ccgaacggcg aaacgcttga
     5521 gttcttctcg tcaaaggctg tctgaacgga aacgtgccgc aaaggatggc atgtgggggg
     5581 acccatgaac tcgcaacaga ggcgcgaata cttccggcag cacctgaaaa gcgttctcgc
     5641 acaggagaat ccgttcctca gacatattac cttccgatac cctcccacag aatccgaatg
     5701 ggggcagttc cctactgaaa tcacagtgaa cgggcagcac ccgggcattc ttgagttgcg
     5761 ggtgaagacg actgctgcaa ccccggaggt tcaagtccgg ttccgcgcaa aagggttcag
     5821 tttctcccct gacgaagacg accccagcta ttcagttttc agcgcggttg cggacaaggc
     5881 cggtgctgat gagcttgcag attttgtgga gtgggcattt cttgtcgctc tggattcccc
     5941 ggatgactat gatccactga tcagaactcc ggtatcagaa caaggacctt ccggcgcgcc
     6001 ggcgaaaccg ggtaaagggt gcagcagcgg atgcagaaac atcgctctcg ctcttctctg
     6061 gttgctgctt gctatttttg cactgcgttt tcttttcggt ggctgaccca ttgcgtggct
     6121 tgttccagaa atgatcgaac tcgaagaaat ccagcgccat ctcgccgcaa cctccgtcga
     6181 tggatggctg ttgtacgatt tccaggggat caaccccgtc gccgcccggg tggccggact
     6241 gggcgagaaa atgcttacgc gccggtggtt ctgtcttatc ccacggcagg gaaaccccga
     6301 atggctggtc aaccagattg agcgccagca atttgccgac gtcgagggag tggtgcgcac
     6361 cttctccacc tggcaacaac tcgtatcggg cctttcttcg ctcctccagg ggcgcagccg
     6421 cgtcgcgatg gaatacagtc cgggcggcac catcccgtac gtgagccggg ttgacgccgg
     6481 caccttggag atcgtgcgca ataccggttg cgctgttgtt tcgtcggcag atctcgtcca
     6541 gtggtgtgaa gcgcggcttt cggcgagtgc gctcgcgacc cacctctatg cggcgaagca
     6601 tctcgaaact gcgaaagaat cggcgttcga attcattttc cgacagatgg aggcgggtaa
     6661 gcgaatttcc gaatacgata ttcagcagga aatcatccgc tacttcgatc tccacggcct
     6721 cgtcaccagt tccccgccga tcgtggcggc agcgcagaac acaagcgatc cgcattacca
     6781 gccaacgtca tcgagagcga agctgctgga ggagggcgac ctggtcctgg tcgacctctg
     6841 ggcaaaaacc cgcgacccgg gcgcggtcta cgcggatatt acgtggatgg cccacgtaag
     6901 tgattcggta ccggacgagc tggcgcgggt gttcgccgcc gtcgtgcgcg cacgtgacgc
     6961 tgcgatagat ttcctcaagc gctcagtggc gagaggtgac atcgttctgg ggtacgaagt
     7021 tgatgacgtc gcccgtggtt ccgtaacgaa ggacgggtac ggagatcgtt tcatccaccg
     7081 gaccgggcac aacatcggca cggaagatca cgggcggggt gtcaatttcg acaattacga
     7141 aacccatgac gacaggcgcg taataccaaa cataacctgc tccatcgaac ccggtctgta
     7201 tttcggtttc ttcggaatgc ggacagaggt aaacgtgcat atcggcgcgc gcagcgtgga
     7261 agtcagtacg gtaccgatgc aacggcagat aattcccctg cagaggcatt tcctgcgaga
     7321 agccacttcg ccttccgagg gcttgtgatg ctcgattacc tccaagaaca gaccgcgcgg
     7381 atcaaccagc aactggaggc ggttctaccg cgctcgatca cggcggaatg ggtggaagcg
     7441 gtgctgggaa gaagcacgta ttccgtggac gaggagactc tgacagccgc gctatcggat
     7501 cccgtgtggg atttcctcga ccgcggcggc aagaggtggc ggccttcgct catgctgaca
     7561 tgttgcgaag cagtatgtgg gagcattgaa cccgcgcgtc ctctggtacc gctgatcgaa
     7621 ctcatccaca atgggaccct ggttcatgat gacctggagg acaacagcga tctgcggcgc
     7681 gggaagccct gtctccacaa gtcgttcggc atcgatatcg caacaaacgt cggcagcgcc
     7741 atgtatttca tacccctcgt aatcctgtac ggccgacgcg cacactactc ggacgcccag
     7801 tgcctgtaca tctacgacct cgtggctcag gagcttgtcc gatgccattg cggccaggcg
     7861 attgatatcg cgtggcacaa cggacgaaga gacgatatca cagaagatga gtatctccag
     7921 atgtgcgcgt acaaaacggg cgtgctggcg aggcttgcgg cgcgtcttgg cgcatacgta
     7981 gggggtgcaa cgccgcaaca ggtggaggaa ctcggtcggt tcggagaaac catcggggtc
     8041 gcgttccaga ttcaggatga cattctgaac ctggttggtg aacgtttcgc agaaggcaaa
     8101 ggacgcgggg aggacatcca cgaggggaag cgcaccctgc tggcgttaca cgcactttct
     8161 cacgccagtt cctcgcaggc ggcacgtctc cgggagattc tggctgcgca tcccacggaa
     8221 cagcaggttc tcgacgaagc catatcgatc atcgccgcgt gcggctcgat cgattacgca
     8281 cgccgcaaag cggtcgctct cgtggaagcg agctgggaaa aggtcgccac cctcgttcaa
     8341 gaatccgacg caaaaaaacg cctccgcgca ttcgcggatt ttgttgttca gagagacctt
     8401 tagggaccgc agccctcagg gagcgggttt cccgaagatc tttggaagag gcagaaacgg
     8461 gcacgccctt tccggaaagg aagctaccgt gcggagaacc acaccacctt ttcgggtcgg
     8521 aatcatcggt tgtgggggca tttctcacgc gcacgcgctc ggttttggga atctgcccgc
     8581 gctgtttgag gttcgtgcag tctgcgactc aaacgaaaag gcggcccgga agctcgcgga
     8641 gccattcggt gaaccgcgca tctgctccga ttggcgagag gtgatcgccg accccgaaat
     8701 cgatgcagtc gacatccttc tgccgcacca cctccatgcg ctcgttgggg tatccgcact
     8761 ccgcgcggga aagcacgtgt gtctggaaaa gccgctggcg atcacccttt cagagggaat
     8821 cgagcttgtt cttgaggcgg aggcctccaa cgcagtgttc atggtcgcat tcaatgaacg
     8881 gcaccgctgg tccgtcagga cgatcaagaa aatcgttgac gaggggcgga ttggcgacgt
     8941 gtacatggtg cgaacggacc acaaccagaa cccgcgtttc gcggcgaatg cctggtaccg
     9001 caacggacaa ctcgccggcg ggggagcgtt gatcggttca gggatccaca tgctcgatct
     9061 gctgcagtgg ttttgtggaa cggcaacgag cgtatttgcg accggcgtga aactcccgga
     9121 agtccttgaa actgagacgg cggcttctgt catcgtcgaa ttcgcgccgc gcaaggaagg
     9181 atgcacggac tgcatcggga cgctcgatat ctcctgggcg gccccgaacc atccatggta
     9241 ccagtttctg gtcgtgtatg gaacgagggg aaccgtctcc acgctgcgtg gtgacgtcgt
     9301 cagcctccgc actgccgggg agcaggagga gttccacgct cccgaaggcg acacgtggac
     9361 agattcattt cgggaggagc tgaggcactg gggggaatgc atcgcgcagg gcacgagacc
     9421 tcttacggac gcacgcgagg catttcgctc gctcgagatc tgcatggccg cctaccgctc
     9481 gatggacaca catactgctg ttcgaatccc gcttccggaa tagaccgatg cgcgtcgcct
     9541 gcatcataaa tccgcgggcg ggaaggcgcc ctcatgcgga acacgtgatt gccgggctgc
     9601 gcagtgttgc cagcatgcac gggtgggaac ttcggaccgt cacaactacg gggccgggcg
     9661 aggctacgca gatcgcacga tctctcgttc ctgccagcga catcatcgtt gccgccggtg
     9721 gcgatggaac ggtacacgaa gtatccgcgg cgctggtcgg aacgcaggta gccctcgcag
     9781 tcatcccgtg tggttccggc aacgggttcg ccagatcctt caaaatcccg ttggacccgc
     9841 ttcgcgcgat tcgctcactg gttgacagcg aacagatcag cgttgatgtt gggaccgtag
     9901 aaggtcgtca ctttttcgcg acagccggca tcggcctcga tgcagaaatc agtcggcgat
     9961 ttgctgcggg gaaccatcgt cggggaatct ggccatattt ccgccacgga ttcgctgcct
    10021 gggtggcgta ctgtcctcag aaagtcacca ttcaatcagg caacatgtcc ctgtcagcaa
    10081 aaccgctcgt cctggccgtt gcgaacacgg agcagtacgg tggcggagcg aaaatcgcac
    10141 ccggtgcatc tccgtttgat ggccttttga acatcaccat cgttgagacg agttcgttcc
    10201 ttcgtctcct gatcgcgttc ccgaggctct ttacgggctc tctggatcgg tcacctctcg
    10261 ttcggcgaat gacggccacg gagttcacga tccatcgcca ggacccgggc ccgtaccacg
    10321 cggacggcga gccattcgac ggaccagcag tccttcattt ctccgttgtg cctcacgcgt
    10381 tgagattgct cgtgccacgg gggaaatacc caggctgacg cgccgtggaa ctcgtacgcc
    10441 gtctttccgg gtagtattgg aagccatcta agatacgttt ttccaagttg gaagcgtgtg
    10501 caatacggcg aagggaggaa accatgttga aacggctgct ggagactgac agaaatgacc
    10561 tcgcggggtt tgtggcgagg gtgtttcttg gaattgtcat gttcccccac ggtgcccaga
    10621 aaatgctcgg atggttcggc gggcacgggt tctcggcgac gatgggattt ttcggttcaa
    10681 tgggaatccc tccggtgttc gcgtttctcg cgatcttcgc ggaatttttc ggcgcactgg
    10741 gagtgatctt cggttttctc acccgggtag ccgcgttcgg cattggcatg gtgatgttag
    10801 tagccatgac agccgtccac ctgcccaacg ggttcttcgc acagcagaat ggcatcgagt
    10861 ttacgctcat tctgttcggc gtcggcctcg cggtaatgat caagggagga ggaaggtggt
    10921 cgctggacga g
//
LOCUS       DAOTNG010000072        10916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5650_length_10916_cov_3.963355, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000072 DAOTNG010000000
VERSION     DAOTNG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5650_length_10916_cov_3.963355"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1067)
                     /locus_tag="P5179_05065"
     CDS             complement(393..1067)
                     /locus_tag="P5179_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRS94622.1"
                     /translation="MIYLVRAELPENRQTSLAGAIPLKIRLEEATGLPCLIRWFSDVS
                     LTEVRRLPVRAIVIAGFAADIETMGEAAFRPLTEAVMGAEVPIMGICGGHQLLGCMFG
                     GDPWQNFPMRPLRPGEPDVSDYHPGMFKEWGFHTVRKVGTDPLFDGLPDDMVFSQSHY
                     CEIRTLPPDFELIATNDNCRVQAMRHRSRPIWGTQFHWERFTDHYPHGRTVMENFFRE
                     AGIATS"
     gene            1785..3599
                     /locus_tag="P5179_05070"
     CDS             1785..3599
                     /locus_tag="P5179_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRS94623.1"
                     /translation="MPKYAVPYAAPPVLERPSNAQFSLGGVVGERVKAALHGWLLPAP
                     DANPALTEMFRDRNRTSSRELVPWAGEFVGKYLIAAQQILRLTGDKRLRSTIGGIVAE
                     LIAAQDEDGYLGPFDKENRLVEKWDVWGHYHVMLALLMYYEASGSLFALSACRKMADL
                     LHQFFVMGNRRMLTRDDPDGEKNYAVSHAVLWLYRITGEARYRELVDWIQKEWDAPPA
                     GQYISSALAGAPVWKFPSHRWESAHDYEAIAELGLLTGDERYARVARHIWWSILEGDR
                     HNTGGFTSGEACQGNPYDQGAIETCCTMAWIAFSIDVLRMTGDPRIADEIELATLNGM
                     VGGQNPAGRWWTYNTPMDGRRVASAQDIVFQSRAGSPELNCCSVNAPRGLGMISEWGF
                     MVSEGGFAVNYYGPSAVTLRRPSGEIVRLQQETSYPVGNEAVVAVGCSPPAEFTLSFR
                     IPAWSKATKVNVNGDAVAGVEPGTYCAITRKWREGDTVKLVFDFSPHVWVGEKECAGK
                     VSLYRGPILLAFDPRFNTMDLDAVPTVDVSNISFEPAAWSGKWPPWLLVRVPTVDGRE
                     LLLCDFANAGSTGTHYCSWIPCVGGEPRAFSRDRAVWV"
     gene            complement(4049..5095)
                     /locus_tag="P5179_05075"
     CDS             complement(4049..5095)
                     /locus_tag="P5179_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94624.1"
                     /translation="MALVPGFFRTYTKERAHIIDPGTGVKITQLTSFPTVHQNLYFHS
                     RSFTPDCKTVLFLSNTTANRAGLNDLYAIDTDGDNLRQLTNGEAVDGIALSPQGQFAY
                     YASGRVVYAQDINTGEAQEVARFAKGVHAGGNATMTDDGKLLCMFASVEGAGQQIGLV
                     SIPEGKAWTVRTDYGTPHLQIEPGKGETAFYYGPRLPRIVKLDGSGEHEIPLDQHNGH
                     SAWLGPKGKVYTGNIYGVNKITMCDQAGGPVETIAEGPYFWHPGCDPTGEWIVSDTNW
                     PDEGLWIINVKTKKKAKLCASMSSNSHPGWSHPHPCVGPGAKEVLFNSDRTGVPHVYA
                     AHVPEELRAKLAAG"
     gene            complement(5137..>5531)
                     /locus_tag="P5179_05080"
     CDS             complement(5137..>5531)
                     /locus_tag="P5179_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94625.1"
                     /translation="LAAPARGIGSIAPDETELELVAGGSSFWHAASSLDGKWIVSDTN
                     WPDVGIQLIHVESGRYACLCKSLGTNSVGQETHPHPSFNRAGTHVLYTSDQTGVSQIY
                     LAAIPDTIRTELETGALTHRQRWPGKVI"
     assembly_gap    5532..5631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5632..6380)
                     /locus_tag="P5179_05085"
     CDS             complement(<5632..6380)
                     /locus_tag="P5179_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94626.1"
                     /translation="MFERGTTFGREVIEMTDPVTGAFIMQLTSAQNASAKLYFELQSF
                     LCDDSTIIFHSQRVPARGAPMELFRVDTDGRNLTQLTDEAHPLGHPVPAWTTRSVYGL
                     RANVLIRLDVDTLEETEIACCDEAVEGASSSMSGDDRFFFTVATMKDGQKTIVRFATD
                     GSEVAVMLRGIPVNHINADPAGTRFTFNRSLDPDDPGRIQICDFDGGNLRAFPFQRFA
                     HRSWLGRTGRVQGPLAAPARGIGSIAPDETEL"
     gene            complement(6404..7444)
                     /locus_tag="P5179_05090"
     CDS             complement(6404..7444)
                     /locus_tag="P5179_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94627.1"
                     /translation="MTDPVTGAFVLQLTAAQNFSTNLYFEHQSFLCDDKTLIFLSQRA
                     PGRGMPYDLFRVDIDGRNMTQLTDEEHPLGHPIPSWDKRIIYGFRKNVLLSLDPDTLE
                     ETELNRCDEATGFGACTLSGDDKYFIAVATMKDGTSAIVRLATDGSDVAVMCEGLPYN
                     HIVANPGKPEFTFSGKGSAEFPQGVMVCNNDGTNLRHFVFQQFAHCAWLGRTGRMQGT
                     LLPPGHAIMAIASDETEPTPIASGVYFWHSAASLDAKWIVADTNWPDIGIQLIHVESG
                     RYACLCKSLGSNGDWTHPHPSFNRAGTQVVFNSDRTGVPQIYLATIPDAIRMELETGA
                     LTHRQRWVGKVI"
     gene            complement(7498..8568)
                     /locus_tag="P5179_05095"
     CDS             complement(7498..8568)
                     /locus_tag="P5179_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94628.1"
                     /translation="MAEMTTYERMKRMYEHREADRVPVTDGPWGSTLERWHREGLPSD
                     IPWDDYFGLDKFAGIGADNSPRFPSRVIEETDEYTIAVSPWGATSKNWKHAGGVPEFL
                     DFKVKDWDTWREAKARMQPTRDRVNWQHLEQNYRKWREEGRWITAGFWFGFDVTHSWF
                     IGTAKVLMAMVEDPEWIADILNHELELDLALFQMVWDEGYRFDAIGWPDDMGYKQHTF
                     FSLPMYRQLIKPVHQRAAEWAHAKGLKVHLHSCGDVHTFIPDLIELGIDMLNPLEVKA
                     GMDPVALKKQYGDRLAFHGGINAVNFTDPDALFAEMKRVIPVMKENGGYVISSDHSVP
                     DAVSFEDFKTFVRLAKELGSYT"
     gene            8796..10214
                     /gene="pyk"
                     /locus_tag="P5179_05100"
     CDS             8796..10214
                     /gene="pyk"
                     /locus_tag="P5179_05100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRS94629.1"
                     /translation="MIRRTKIVCTIGPQTASIEQLRALVDAGMNVARVNFSHGTHEGH
                     RSVIRAVRALRGQYGEPVGVLADLSGPKIRLGKLPGGSCRVETGQTIILTCDTGGNCG
                     DRFPINYPYLSEDVKVGDSVVMNDGDVSARVTEVRGGDVVCVVEDGGMLGDNKGVNFP
                     DSALRVDAITEKDRRDVRFALEEKVDFLALSFVRRPEDIVSLRGLVAGGSHTPRIIAK
                     IEKREALENFDAILAEADGIMVARGDLGLETELARVPMVQKDLIARARAAGKPVITAT
                     QMLESMIVNPVPTRAEVADVANAILDGTDAVMLSGETAVGRFPVDTVRMMARIAAATE
                     AAHQPANGFRRNADRGVLNPSEAIGQSTAQMAWDLHVVAIVVCTDSGKTGRVVSRFRP
                     AIPIVGATPNEEVARQLTLSWGVVPALLEPITSTDELIAHAFATVKGTGIAKDGDQVV
                     ITAGIPFGGGGMTNLLLVQQIA"
     gene            10332..>10916
                     /locus_tag="P5179_05105"
     CDS             10332..>10916
                     /locus_tag="P5179_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94630.1"
                     /translation="MRRSISLILLLALVSIVGCGLFSKKQAANYVTPEGWVGATAKKG
                     SFTLDAVLLDDSSYGLEGAVRFSWWKVFNVTVENRSRQPVSLPLSSFSLVDASGTEQK
                     PLSAEEVQAYQSYVMGPLLSYQRSAVRQAIWSEKEIAPGAFAVGYLFFPRKRHATPYS
                     LVLDPSPNKRKDELVLAFPAQYVEPELPAVAAARD"
BASE COUNT         1901 a         3087 c         3721 g         2107 t
ORIGIN      
        1 ctcgttgaga atacttcggc aggccggctg tggccaagtg cgggggtggg gtgatgagcc
       61 cgaacaggtg tcgcaagacg cggatataca tgcgttgtta agtccactcc ccggccgccg
      121 tgcttacttc agtgcgcgct gcccaagggt ggggcgccgg tagtcgagtg gcgaaccggg
      181 aaggaacgga ggcgaagcga cgctcaccgg ttccatgtat cgagacgcac cggtgcggta
      241 catgggccgc gcacggtctg atacgtccta tacgacctat ggcgcgcatg catggccaag
      301 gcgccgaagt gtgcccgagg gtgaaaaatc ctggtacgaa tccgcaactt ctgcacaaag
      361 actcttgaca cgaagacgac gttttgcctg tgtcatgaag tggcaatacc ggcttcccga
      421 aaaaagttct ccatcaccgt acggccgtgc gggtagtggt cggtgaagcg ctcccagtgg
      481 aactgcgtgc cccagatcgg acgcgaacga tggcgcatgg cctgcacacg gcagttgtcg
      541 ttcgtggcga tgagttcgaa gtcgggtgga agtgtcctga tctcgcagta gtggctctgc
      601 gaaaacacca tgtcatcggg caggccatcg aagagcgggt cggtgccgac ctttctgacg
      661 gtgtggaatc cccactcctt gaacatgccg gggtggtagt cgctcacgtc gggctcgccg
      721 gggcgaagcg gccgcatggg gaagttctgc cacgggtcgc cgccgaacat gcagccgagg
      781 agctgatgcc cgccgcagat gcccatgatc ggtacttcgg cacccattac cgcctcggtc
      841 agcgggcgga aggccgcctc gcccatcgtc tcgatatcgg cggcgaatcc ggcgatgacg
      901 attgcgcgga cgggaagcct gcgcacttcg gtgagtgaca cgtcggaaaa ccaacggatg
      961 aggcacggca ggccggtggc ttcctcgagg cgaattttga gcggaatggc accggcgagg
     1021 gaggtctggc ggttctcggg cagttcggcg cgaacaaggt agatcatggc ggctcctgac
     1081 gggtggataa atggtgctgt ttgctctgct gatacgtgag tggtaggttg aaaaaacatc
     1141 cggagcccgc ttcgaggcaa tgggatgcgg cctgcgccgt ccggatggaa gaagaccata
     1201 gtgtcgtgca acgcgcccgg ccctgcgagc cgggctacgg tatttcgcgc cgttggcgct
     1261 gcctgcgcgg cccttcgaca ggctccggcg cgagggttcg ggagaaaact ggacccgacg
     1321 gagcgggtcc ctccagtgca acgtccctgg cggaggggca ggcgcatggg ttggagggtc
     1381 acgctctgtc gtgaccaatg accttgctgg cgccagtctg cacggcgttt caatgcatcg
     1441 cgacggcact tcgacaggct tcctgcggcc ccgcaggctc aggggcctcg caggatgggc
     1501 gcccgccttc accggcgcca gaaggcctga agggccggaa tagctcagcc cggggcaacg
     1561 tcccgggcgt gggaaaaaag gggacctggc gaagaacgaa cggtgttctc cgcggatccc
     1621 tctagggcca gcgcggtcgt tgagcttgtc gaaacgccgc gcggggcttc ggaaggtcgc
     1681 ccggtgggcg gcaatctgcc tggcggcact tcgacaggct cagtgaccgc gggagcaact
     1741 ccggccattg cgcgcatttt cacccatcat cgggggtttt ccgtatgccg aaatacgctg
     1801 ttccctatgc cgcgccgccg gtacttgaac ggccatcgaa tgcgcagttt tcgctcggcg
     1861 gtgttgtcgg cgagcgggtg aaggccgccc tccacggctg gctcctgccc gcgcccgatg
     1921 ccaaccccgc gctgaccgag atgttccgcg accgcaaccg gacgtcctcg cgggagcttg
     1981 tgccgtgggc gggtgagttt gtgggtaaat acctcatcgc cgcacaacag atactgcgtc
     2041 tgaccggcga caagcgcctc cgctccacca tcggcggcat cgttgccgaa ctcatcgccg
     2101 cgcaggacga ggacggctac ctcggcccgt tcgacaagga aaaccgcctc gtggaaaagt
     2161 gggatgtgtg ggggcattac cacgtcatgc tggcgctgct catgtattac gaggcctccg
     2221 ggtcgctctt tgccctctcc gcatgccgca aaatggccga tctgctccac cagttttttg
     2281 taatggggaa tcggcggatg ctcaccagag acgatcccga cggcgagaag aactatgcgg
     2341 tgtcgcacgc ggtgctgtgg ttgtaccgca tcaccggcga agcgagatac cgcgagctgg
     2401 tggactggat ccagaaagaa tgggatgcgc cgcctgccgg gcagtacatc agctccgcgc
     2461 tggccggcgc gccggtgtgg aagttcccct cgcaccggtg ggaaagcgcg cacgattacg
     2521 aggcaatcgc cgaactgggg ctgctgaccg gcgacgagcg gtatgcgcga gtcgcccggc
     2581 acatctggtg gagcatcctc gagggcgacc ggcacaacac cggcgggttc acctccggcg
     2641 aagcgtgcca gggtaacccg tatgatcagg gcgccatcga aacctgctgc accatggcct
     2701 ggatcgcgtt ttccatcgac gtgctcagga tgaccggcga cccgcggatt gcggacgaaa
     2761 tcgagcttgc gacgctgaac ggaatggtgg gcgggcagaa cccggcgggg cgctggtgga
     2821 cgtacaacac gccgatggac ggccggcggg tggcctccgc gcaggatatc gtgtttcaat
     2881 cgcgcgccgg cagcccggaa ctcaactgct gctcggtgaa tgcaccgcgc gggctgggaa
     2941 tgatctccga gtgggggttc atggtgagcg aaggcgggtt cgccgtgaac tactacgggc
     3001 cgtccgccgt cacgctgcgc cgcccgtccg gcgagatcgt gaggcttcag caagaaacca
     3061 gctaccccgt gggcaacgag gcggtggttg ccgtgggctg ttcgcctccc gccgagttca
     3121 cgctgtcgtt ccggattccg gcgtggtcga aggcgacgaa ggtcaacgta aacggggacg
     3181 cggtcgccgg ggtggaaccc ggcacgtatt gcgccatcac ccgcaaatgg cgcgaaggag
     3241 atacggtgaa gctggtgttc gatttctccc cgcacgtctg ggtgggcgaa aaggagtgcg
     3301 ccggcaaggt gtcgctctac cgcgggccga ttctgctggc gtttgacccg cggttcaaca
     3361 caatggacct ggatgccgtg ccaacggtgg atgtgagcaa catatccttc gagccggcgg
     3421 cgtggtcagg gaaatggccg ccgtggttgc ttgtccgcgt gcccacggtg gacggccgcg
     3481 agctgctcct gtgcgatttc gcgaacgccg gaagcaccgg cacgcactac tgctcgtgga
     3541 taccgtgcgt gggtggggag ccgagggcgt tttcgaggga cagggcggtg tgggtgtagg
     3601 gaagtcggcg ttcgggcttt ggagtgcgca cgcgggcttg cgctttcaaa gcggaggcgc
     3661 gcctccgcaa tccaaagaag caggttgttt ctggattccc gccttcgcgg gaatgacggc
     3721 atatgtgagc gccggaggaa acgtgcccgg ccctgcgagc cgggctgggg tattacgcgc
     3781 cgttggcgct gcctgtgttc gaatgcggct ggcaaggcct gaagggccgg gatagctcag
     3841 cccggggcag agtcccgggc ggggggcgtg tccggggtgg agggtcacgc tttgtcgtga
     3901 ccgttcggcg atgcgggggc aaaaggcccc gacggagcgg ggccctccaa ggcggtcgca
     3961 tcaaacccgt gcgcggccct tcgacaggct cagggaccgg gcagggcggc ggtctcggcg
     4021 agaccgccct acctccccgg ttggagcgct atcctgcggc cagcttggcg cgcaattcct
     4081 ccggcacgtg ggcggcgtag acgtgcggga cgccggtgcg gtcggaattg aacagcacct
     4141 ctttcgcgcc ggggccgacg cacgggtgcg ggtgcgacca tccggggtgg ctgttgctgc
     4201 tcatggaggc gcacagcttc gcctttttct tcgtcttcac gttgatgatc cagaggccct
     4261 cgtccggcca gttcgtgtcc gagacgatcc actcgccggt ggggtcgcag ccggggtgcc
     4321 agaagtacgg gccctcggcg attgtttcca ccggtccgcc tgcctgatcg cacatggtga
     4381 tcttgttcac cccgtagatg ttccccgtgt agaccttgcc cttgggcccg agccacgctg
     4441 agtggccgtt gtgctggtcg agcggtatct cgtgctcgcc cgagccgtcc agcttgacga
     4501 tgcggggcag gcgggggccg tagtagaacg ccgtttcgcc cttgcccggt tcgatctgga
     4561 ggtgcggggt gccgtagtcg gtgcggacag tccacgcctt gccttcgggg atggagacga
     4621 ggccgatctg ctggccggcg ccttctactg aggcgaacat gcacaggagt ttgccgtcgt
     4681 cggtcatggt ggcgttgccg cctgcgtgga cgcccttcgc gaagcgtgcc acctcctgcg
     4741 cctcgccggt gttgatgtcc tgcgcgtaga cgacgcggcc tgaggcatag tacgcgaact
     4801 gcccctgcgg gctcagggcg atgccgtcca cggcttcacc atttgttaac tggcgcaggt
     4861 tgtcgccgtc ggtgtcgata gcgtagaggt cgttcaggcc ggcgcggttg gcggtggtgt
     4921 tggagaggaa cagcaccgtc ttgcagtcgg gcgtgaacga gcgggaatgg aagtagagat
     4981 tctggtgcac cgtggggaac gacgtgagct gggtgatttt cacgccggtg ccggggtcga
     5041 tgatgtgcgc gcgttctttc gtgtacgtgc ggaaaaagcc gggtaccagt gccatactgt
     5101 gtgctcctga gggtgtaggg tgaaacgaat gctgagttat atgacctttc ccggccagcg
     5161 ctggcgatgc gtcagcgcgc cggtttcaag ttcggtgcgg atggtgtccg ggattgccgc
     5221 gaggtagatc tgcgaaacgc cggtttgatc cgaggtatac aaaacgtgcg ttcccgcgcg
     5281 attgaaggac ggatgcgggt gcgtttcctg cccgacggaa ttggtgccga gggatttgca
     5341 caggcatgcg tagcggcccg attcgacgtg aatgagctgg atgccgacat cgggccagtt
     5401 ggtgtcggag acgatccact ttccgtcgag gctggaggcg gcatgccaga aggatgagcc
     5461 accggcaaca agctcaagtt ccgtctcgtc gggggcgatg ctgccgatgc cccgcgccgg
     5521 ggcggccagc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagttccgtc
     5641 tcgtcggggg cgatgctgcc gatgccccgc gccggggcgg ccagcgggcc ctgcacgcgc
     5701 ccggtgcggc caagccatga acggtgtgcg aaccgctgga aggggaatgc ccgcaagttg
     5761 ccgccgtcga aatcacagat ctggatacgg ccggggtcgt cggggtcgag actccggttg
     5821 aacgtgaacc gggttccggc cgggtccgcg ttgatgtgat tgacgggaat gccgcgaagc
     5881 atgacggcga cttcggagcc atcggtggcg aaccggacga ttgttttttg cccgtctttc
     5941 attgtggcga cggtgaagaa gaatctgtcg tcgccggaca tgctcgagga ggcgccttcc
     6001 acggcttcgt cgcaacaggc gatttcggtc tcctcaagcg tatccacatc gaggcggata
     6061 agcacgttcg cccgcaggcc gtagaccgaa cgggtggtcc acgcgggaac cggatggccc
     6121 agcgggtggg cctcgtcggt gagctgggtc aggttgcggc cgtcggtgtc cacgcggaac
     6181 agttccatag gggcaccacg cgccggcaca cgctgcgagt ggaagatgat ggtgctgtcg
     6241 tcgcacagga acgactgcag ctcgaaatag agcttggcgc tggcgttctg cgcggaggtg
     6301 agctgcatga tgaatgcgcc ggtgaccggg tcggtcatct cgatcacttc gcggccgaag
     6361 gtggtgcccc gttcaaacat ttcgtgctcc gagagaaccg gaatcagatc actttcccta
     6421 cccagcgctg gcgatgggtg agcgcgccgg tttcgagttc cattcggatc gcgtcgggaa
     6481 tcgtcgcgag ataaatctgc gggacgccgg tgcggtcgga gttgaagaca acctgcgtgc
     6541 cggcgcggtt gaacgacgga tgcgggtgcg tccagtcgcc gttgcttccg agcgatttgc
     6601 acaggcaggc atagcggccc gattcgacgt ggatgagctg gatgccgata tccggccagt
     6661 tggtgtcggc gacgatccac ttcgcgtcga ggctggcggc tgagtgccag aagtagacgc
     6721 cggatgcgat gggtgtgggc tctgtttcgt cggaggcgat ggccatgatg gcgtggccgg
     6781 gcggcaggag cgttccctgc atacggccgg tgcgccccag ccaggcgcag tgggcgaatt
     6841 gctggaagac gaagtgccgg aggttggtcc cgtcgttgtt gcagaccatc acgccctgcg
     6901 ggaactcggc actgcccttg ccgctgaagg tgaactccgg tttgccgggg ttggcgacga
     6961 tgtggttgta gggcagaccc tcgcacatga cggctacgtc cgagccgtcc gtcgcaagcc
     7021 gcacgatggc gctggtgccg tctttcatgg ttgcaacggc gatgaagtat ttgtcgtcgc
     7081 cggagagcgt gcatgcgccg aagccggttg cctcgtcgca gcggttcagt tcagtctcct
     7141 caagcgtatc ggggtcaagg ctcagaagca catttttgcg gaagccgtag atgatccgct
     7201 tatcccacga ggggatcgga tggcccagcg ggtgttcttc gtcggtcaac tgggtcatgt
     7261 tccggccgtc aatgtccacg cggaacaggt cgtacggcat gccgcggccg ggagcgcgct
     7321 gagagaggaa gatgagcgtc ttgtcgtcac acaggaacga ctgatgctcg aagtagaggt
     7381 tggtgctgaa gttctgtgca gcagtaagct gcaggacaaa tgcgccggtg accggatcgg
     7441 tcatttctac cgtttcgcgg ccgaaggttc tgcctcgttt cgacatggtg gttttcctta
     7501 cgtgtaagac ccgagttctt ttgccaatct gacgaaggtc ttgaagtcct cgaagctcac
     7561 ggcatcggga accgagtggt cagagctgat cacgtaccca ccattttcct tcatcaccgg
     7621 aatcacgcgt ttcatttccg cgaacagggc atccggatcc gtgaagttga cggcgttgat
     7681 acctccgtgg aacgcgagtc tgtctccgta ctgctttttg agcgcgaccg ggtccatgcc
     7741 tgccttcact tccagcgggt tcagcatgtc gatgcccagt tctatgaggt cgggtatgaa
     7801 ggtgtgaacg tcgccgcagg agtggaggtg caccttgagg cctttcgcgt gcgcccattc
     7861 cgctgcccgc tggtgcaccg gtttgataag ctgccggtac atcggcaacg aaaagaacgt
     7921 gtgctgcttg tagcccatgt catccggcca gccgatggcg tcaaaccggt agccttcgtc
     7981 ccacaccatc tggaacagcg ccaggtccag ttccagctca tggttgagga tgtcggcgat
     8041 ccattcgggg tcctccacca tcgccatgag tacttttgcg gtgccgatga accatgaatg
     8101 cgtcacgtca aaaccgaacc agaagcctgc ggtgatccag cggccttcct cgcgccactt
     8161 cctgtagttc tgttcgaggt gttgccagtt cacgcggtcg cgcgtgggct gcatgcgggc
     8221 tttcgcttcg cgccaggtgt cccagtcttt cactttaaag tcaaggaatt ccgggacgcc
     8281 gcccgcgtgt ttccagtttt tcgacgtagc gccccacggg ctgacggcaa tcgtgtattc
     8341 gtcggtttcc tcgattacgc gggacgggaa acggggactg ttatccgcac caatgccggc
     8401 gaatttgtcg aggccgaagt agtcgtccca cgggatgtcg ctgggcaggc cttcgcggtg
     8461 ccagcgttcc agcgtggagc cccacggtcc atcggtaacc ggaacgcggt cggcttcccg
     8521 gtgctcgtac atgcgcttca tgcgctcgta ggtagtcatt tctgccatgt tcgcagttct
     8581 ccctgaggcg ggatctggtt gctcggtgtg tggccgaatg ccggaaaaaa agatacctgc
     8641 cggtccggca agaccaaacc aaccttgtaa gacgggacgg tgcgtcttac tttgtgaacc
     8701 gatggcgtac cctgccccgc tcacgaaaaa cacatccaag agccttaatc tgaagagacg
     8761 acacgtaagc tggatttttc cgtgggaagg aaaccatgat ccgtcgaacc aagattgtct
     8821 gcacgatcgg cccgcagacg gcctcgattg aacaactgcg cgcgctggtt gatgccggga
     8881 tgaatgtagc gcgcgtgaat ttctcgcacg gcacgcacga ggggcaccgg tctgtcataa
     8941 gggcagttcg tgcgttgcga gggcagtacg gcgaaccggt gggagtgctg gcggatcttt
     9001 cggggccgaa aatccgcctt gggaagctgc ccggcggctc gtgcagagtg gaaaccggcc
     9061 agacgatcat ccttacctgc gatacagggg gaaactgcgg cgaccggttc ccgatcaact
     9121 acccctatct ttccgaggac gtgaaggtcg gcgactcggt ggtaatgaac gacggcgacg
     9181 tgagcgcgcg agtgacggag gtccgcggcg gggacgtggt gtgtgtggtg gaagacgggg
     9241 gtatgctggg agataacaag ggggtcaatt tcccggactc cgcgctccgc gtagacgcga
     9301 tcaccgagaa ggatcggcgc gacgtgcgtt ttgcgctgga agagaaggtt gattttctcg
     9361 cgctttcgtt cgtgcggcga ccggaggaca ttgtttcact gcgcggactt gttgcgggag
     9421 gctcccacac gccgcggatc attgccaaga tcgagaagcg ggaggcgctg gagaacttcg
     9481 atgccatact tgccgaggct gacggcatca tggtggcgcg gggggatctc ggcctggaga
     9541 ccgaactggc gcgcgtgccg atggtgcaga aggacctgat cgcccgggcg cgggccgcgg
     9601 gaaagccggt catcacggcg acacagatgc tggaatccat gattgtgaat ccggtgccaa
     9661 cacgagccga ggtcgccgat gtggcgaacg caattctgga cggcaccgac gcggtgatgc
     9721 tgagcgggga gactgcggtg ggacgtttcc cggttgacac ggtgcgcatg atggcaagga
     9781 ttgccgccgc gacggaagca gcacaccagc ctgccaatgg cttccggcgg aatgcggacc
     9841 gaggtgtgtt gaacccatcc gaggcaatcg gccagagcac cgcgcagatg gcgtgggacc
     9901 tgcacgtggt ggcgattgtg gtgtgcacgg attcgggaaa gaccggccgg gtggtcagcc
     9961 gtttccggcc cgcgatcccc atcgtaggcg caacgccgaa tgaggaagtc gcccgccagt
    10021 tgacgctttc atggggagtt gttccggcgc ttctggaacc gatcacctct actgacgagc
    10081 tgattgcgca tgccttcgcc acggtaaagg gcacaggcat tgccaaagac ggtgatcagg
    10141 tagtgattac tgcgggcatc ccgtttggcg ggggcggtat gacgaacctg ctgcttgtcc
    10201 agcaaatcgc ctgattgcgg gcgcgcgtgg aaaaacttgg cgtgcacttg cacttgcgac
    10261 atgtattttc gtactctcac actcgtgaaa agccggcatt gtgttggacg tttttccaag
    10321 gggacacgag agtgcgacgc agcatttccc tgattttgtt actcgcgctc gtttccattg
    10381 tggggtgcgg gcttttctcg aaaaagcagg ccgcgaatta cgtaacgcca gagggctggg
    10441 tcggcgcgac ggcgaaaaag ggttcgttca cgcttgacgc ggttctgctg gacgattcca
    10501 gctacggtct tgagggcgcc gtgcggtttt cctggtggaa ggtgttcaac gtaaccgtgg
    10561 aaaaccgttc gcgccagcct gtcagccttc ccctgtcctc gttttccctt gttgatgcct
    10621 ctggcaccga acagaaaccg ctttccgccg aggaggtgca ggcataccag agttatgtga
    10681 tggggccgct tttgagctac cagcgcagcg ccgtccgaca ggcaatctgg tcggaaaagg
    10741 agatcgcgcc gggggcgttc gcggtcggct acctcttttt cccgcgcaag cggcatgcga
    10801 cgccgtacag ccttgttctg gacccaagcc cgaacaagcg caaagatgaa ctggttctgg
    10861 cgtttccggc acaatatgtg gagccggagc ttcctgcagt ggccgcggct cgtgac
//
LOCUS       DAOTNG010000073        10885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5665_length_10885_cov_5.335272, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000073 DAOTNG010000000
VERSION     DAOTNG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5665_length_10885_cov_5.335272"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="P5179_05110"
     CDS             complement(<1..640)
                     /locus_tag="P5179_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94631.1"
                     /translation="MRADGYCFPRAPGFRRCLVATLIALLAGQVPVSAASRTLPAANG
                     MSIVGPWRYQSLWKALRKGRGVIVGTQSGECISGRFVYGGATYVEVSSEDGLREIPVN
                     SIAYVVNVVDLSHARDGAIVGGLIGFGAGVALSYFTRSQTAATPSSASRRARVLDGDE
                     SSDPENGSSTSAPYSDSQSSATTGEDGSSAGADTPSESTAGSDPATGSSASST"
     gene            complement(912..1568)
                     /locus_tag="P5179_05115"
     CDS             complement(912..1568)
                     /locus_tag="P5179_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRS94632.1"
                     /translation="MRSLLMIGLGSFGHHLVSRLAGSNFYIAAIDSDPARVEMVKTVV
                     QKPLIGDASSVDTLKELGVSSFDYVVVSVGDRLDTSILTCLHLRELGAKNIIVKAAHN
                     DHVRLMDMLNVSRVIFPEREAAYQLAHELADLNVLRSIAIEPGISLVEIAVPSEFEGK
                     TLADLGLPTRFGVQVVLIRQLIPEATILPRADFVIKASDTLVIIGTDEAIEKLQQMTT
                     "
     gene            complement(1574..2965)
                     /locus_tag="P5179_05120"
     CDS             complement(1574..2965)
                     /locus_tag="P5179_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRS94633.1"
                     /translation="MTPHRAGFGRRFRLSWASPGVFAGSFALLILAGSLLLWLPVSVR
                     GPGLPYLDALFQSTSAVCVTGLATVDIGTRLSLAGQWVILALIQIGGLGIMTYSLLLV
                     TIVQRSGSPDQSEWLANVFTSDRRLPPRRMLRWIFLVTAVCEAVGALLLTFAFLRQHQ
                     QLSDAAFDGVFHAVSAFCNAGFSTFSYSLLPFRDDLLVNVIVMVLIVTGGLGFIVTFE
                     LWRLVTGRRRWFKLLLQTKLVLFATLILILSGAVAIFVLEMYGVFGGLGLKSRVLASL
                     FQSVTARTAGFNTVDIAALSNDTLMILIVLMFIGAAPGSCAGGVKVTTVGVLVLSVFA
                     RIHDRPKPEAWNRSVPGDTIARAAALFFGAMLLIAVGTLALQLTELAGVTVEASRAKF
                     LPLLFEVTSAFGTVGLSTGITPTLTPAGKIVIIGIMFVGRVGPLTLAATLIGKASRRR
                     AALMYPEEDLMIG"
     gene            3136..4395
                     /locus_tag="P5179_05125"
     CDS             3136..4395
                     /locus_tag="P5179_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94634.1"
                     /translation="MTPKTICRSVHAMFTRNLALLWIGQTISMAGDSVYTKALIWLLL
                     DMTGSSGTTGLVAVSAYLPTLLTGVWAGAVVDRSDHRRVMIAADGVRAMLVLLVPILF
                     AAGALTPSLLFCITFLVATGATFFNPARDALIPSLVSERTHLLRANALIQSSWMFALL
                     LGPAVAMALLSILGARTQHLFVFGAAAYLVSLVCIVALDTPRKTQDSARGPSTLHRDL
                     AEGVRYAFKNRVVRGLLLITAADNLFIMGPAGVGMAVFVREVLKRDIGSLMAIEVCYA
                     IGMIAGTALLPLWNKRLGYGKILIAGMVLDGATFVPLLWVDSLWGTMATIVIHSLAIP
                     LLVVPRPALVQHIVPVNLQGRVFSMIGVTVVGFTAISTGLTGLVTEWIPMPQVYAFIG
                     VAAALCGVWGYTMKDLREAVSASRVSG"
     gene            4588..5934
                     /gene="tilS"
                     /locus_tag="P5179_05130"
     CDS             4588..5934
                     /gene="tilS"
                     /locus_tag="P5179_05130"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRS94635.1"
                     /translation="MAECVRNQELLVPGASVLLAVSGGADSVALALVFHALAAEWHLS
                     LRIAHIHHGLRGREADGDARWVAEFAAALDVPCDVVHVDVPALKEKERGSIEDIARTA
                     RYRELKRVQSAYGMEVLATAHHLDDQAETVLHHLLRGSGITGLGGMPWKTDEGHIRPL
                     LEISRKEIRDWLRRWDVPWREDSSNASLTLTRNRIRHELLPLLQKEWNPEVSRALYRL
                     ADVARAEDEAFEKWAEKALDDALVSKTRDLVLLDIPCLKRYPLGLRRRVYRRAVLGLS
                     SIEAPLSYAETAALDSLHERRTQVSFRRRFRAWSTRSHVAFESFDAWRGPTELLPGTR
                     TEVPSWGAFLVTTAAGCFLCPGTDLQCQASFSSDELAMPLHVRRWRAGDRVRWGASEK
                     LVADLLRGKKRNADPRTAVVVCDSRRVVWVPDLGDIRVTAKKDTNYVTIIRESWRV"
     gene            5927..6490
                     /gene="hpt"
                     /locus_tag="P5179_05135"
     CDS             5927..6490
                     /gene="hpt"
                     /locus_tag="P5179_05135"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRS94636.1"
                     /translation="MSSRDSRLPEVLIPAEDIARRVTELGRKLSADYATVDVGNAGAE
                     ELVVLGVLKGAFMFLADLVRAVSIPIVVDFVRAASYGSGRVSSGDVRIEPIAWPYGEG
                     RDVLVVEDVLDTGKTLDAVVRALEGFRPRSVKTLALLRKPEAKWPVDYVGFEIEDRFV
                     VGYGLDDAGKWRQLPYIGVPRDGGERR"
     gene            complement(6687..6983)
                     /locus_tag="P5179_05140"
     CDS             complement(6687..6983)
                     /locus_tag="P5179_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94637.1"
                     /translation="MILTEFTLPDAMYSVVNSVYEIVLRAREPAMVLQKTTARRRSRI
                     TMVTVRPAVQGWRKRRRFRGETLFLNLRACGVGFSGLRCSRNSASPLTADGRAD"
     gene            6868..8601
                     /gene="ftsH"
                     /locus_tag="P5179_05145"
                     /pseudo
     CDS             6868..8601
                     /gene="ftsH"
                     /locus_tag="P5179_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
     assembly_gap    9079..9178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9179..9458
                     /locus_tag="P5179_05150"
     CDS             <9179..9458
                     /locus_tag="P5179_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94638.1"
                     /translation="RTKAQSQPLEAFPQAHASEGNSQGSREPEEGSTISRTVSGEQIS
                     AADMAENDRQEAIGGAQENTPQSADTNGDIEPGAADTLAKVDEAVGST"
     gene            9506..10255
                     /gene="cdaA"
                     /locus_tag="P5179_05155"
     CDS             9506..10255
                     /gene="cdaA"
                     /locus_tag="P5179_05155"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRS94639.1"
                     /translation="MDITVADVLDVALLTFIFYKSFVLLRGTRSAPMVIGLIILLAIA
                     VIAERAEMVGSNWLLTQVKTVWLIGLVIVFQTELRRLLTYLGQNRLFRPFLAEAPDQT
                     VNEVVAACKTLSEQGIGALIVITRDVGLAGILETGTRIRAEVSASLIVSLFVPKTPLH
                     DGAVVISGNQIEAAKCILPLTQNAVDPSLGTRHRAALGVSEESDAVVIVVSEETSKIS
                     VAVDGVLERALSPEELRARLFRELNPQGRKA"
     gene            10363..>10885
                     /locus_tag="P5179_05160"
     CDS             10363..>10885
                     /locus_tag="P5179_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94640.1"
                     /translation="MPERLFPWVLLLILFFSPPSRGEVKVLNSDADGLDVEITAPLEI
                     DGANPAKLRIPGWETTSDPSQPGVPYLVVPIVVPSEGAVSASVTAQTDTFARCGPVEK
                     GSGDRPIGLDDEVAGAAAWSDPGRQLVSIEITGVARDVRMARLVVCPVSVVEGGVRWT
                     KSLRVAVRFASEST"
BASE COUNT         2213 a         3090 c         3325 g         2157 t
ORIGIN      
        1 cggtagaaga ggcgctcgaa cccgttgccg ggtcgcttcc ggcagttgat tccgaggggg
       61 tatccgctcc cgcgctggaa ccatcttccc ccgttgttgc ggaggattgg ctgtccgaat
      121 atggggcgct ggtgctggag ccgttttcgg ggtcactgct ctcgtcaccg tccagtaccc
      181 tcgctcggcg ggaagcggag gacggggtgg ctgctgtctg gcttcgcgtg aagtacgaga
      241 gggcaactcc ggcgccgaag ccgatcagtc cgccaacaat cgcgccatct ctggcgtggg
      301 agaggtcaac gacgttgacg acgtacgcaa tcgaattgac cgggatttcg cgtagcccgt
      361 cttccgagct cacttccaca tacgtggcgc ccccgtacac gaatcttccg gagatgcatt
      421 cgcctgactg cgtccccacg atcacaccgc gacctttcct gagcgccttc cacaggctct
      481 gataacgcca cggaccgacg atcgacatac cgttcgcggc gggtagtgtc cggctggcag
      541 cgctcacggg cacctgcccg gccagcaacg cgatcagcgt cgcgacaagg cagcggcgaa
      601 aaccgggtgc ccggggaaag caataaccgt ccgcccgcat ggttctcagt tcctcggtca
      661 gaattcgtga tcacgcgtgt gccactgtcc tgtggggcaa aactgatgcc acatgacgat
      721 ggtttcgcca gcaaactgca gcgcagattc tgtccggttc cacggagacg ggaagacacg
      781 tacagagacg gcaactgcgt gttcagactg gtgtggtgcg cagggcggga acccgtcggg
      841 ttgcacacgc ggaaacgaac acgtggtgcg tgcccggagt atccattcgg atacttgtca
      901 acgggcggaa ttcaggtggt catctgctgc aacttttcga ttgcctcatc ggtaccgatg
      961 atcacgagtg tgtcacttgc cttgatgaca aaatctgccc gcggcagaat agtggcctcc
     1021 gggataagct gccgtatcaa taccacctgg acaccgaatc tcgtgggaag ccccaagtcc
     1081 gcgagcgtct ttccctcaaa ttcgcttggc accgcgattt ccacaaggct gataccgggt
     1141 tcaatcgcga tggaacgaag aacattcagg tcagccagct catgagcaag ctggtatgct
     1201 gcttcgcgtt cgggaaaaat cacccgggag acgttcaaca tgtccatcag tcggacatgg
     1261 tcgttgtgcg cggccttcac gatgatgttc ttggcgccca gctcgcggag atggagacac
     1321 gtgagaatac tggtgtccag tcggtcgccc acgcttacga caacgtagtc gaacgacgac
     1381 acaccgagtt ccttcaatgt gtcaacggat gatgcgtcgc ctataagggg cttttgaacg
     1441 accgttttca ccatttcaac ccgcgcggga tccgaatcaa tcgcggcgat gtaaaaattg
     1501 gatcctgcca ggcgacttac gaggtggtgg ccgaaggatc cgaggccgat catcaagagt
     1561 gagcgcatgg agtctaccct atcatcaagt cttcttcggg gtacatgagt gccgcccgtc
     1621 tgcgtgatgc cttcccgatc agtgtcgcgg caagcgtcaa cggtccgact ctcccgacaa
     1681 acatgatacc aatgatcacg atttttcccg caggcgtcag cgtcggcgtg atccccgtac
     1741 tcagcccgac ggttccaaac gccgatgtta cctcaaaaag caggggaagg aacttggcgc
     1801 gggaagcttc caccgtcacg cccgcaagtt ccgtgagctg caacgcaagg gtgcccactg
     1861 cgatcagcag catcgcacca aaaaaaagcg ccgccgctcg cgcgatggtg tctcccggaa
     1921 cgctcctatt ccacgcttct ggcttcggtc ggtcatgaat ccgggcgaaa acggacaaca
     1981 caagaacgcc caccgtggta actttgaccc cgcccgcgca cgaaccggga gcggcgccaa
     2041 tgaacatcag cacgatcaga atcatcagcg tgtcgttgga caaggccgcg atatccacgg
     2101 tgttgaaacc tgcggtccgc gctgtcacag attggaaaag cgaagcaaga acgcgggatt
     2161 tcagccccaa tcccccgaac accccgtaca tctcgagcac gaagatggcc accgcaccgg
     2221 acagaatgag tatcagggtt gcaaacagaa ccagcttcgt ctgcagaagg agcttgaacc
     2281 atcggcgacg acccgtgacg agacgccaca gctcaaacgt aacgataaat ccgagaccgc
     2341 cggtaacgat caacaccatg acgatcacgt tgaccagaag atcgtcgcgg aaaggcagga
     2401 gactgtaact gaaggtcgag aaacccgcgt tgcagaacgc acttaccgca tggaaaacgc
     2461 catcaaacgc ggcgtccgac agttgctggt gttgcctgag aaacgcgaag gtcaacaata
     2521 atgcacccac cgcctcacag accgcggtca caaggaatat ccaccggagc atccgtctgg
     2581 gaggaagcct ccggtcgctg gtaaatacat tggccagcca ctcgctctga tcgggcgatc
     2641 cgctccgttg aacgattgtt accaggagca gtgaatacgt cattatcccg agtccaccga
     2701 tctggataag cgccagaatc acccattggc cggcgaggct caatcgcgtg ccgatatcca
     2761 ccgtcgctag tccggtcacg cacaccgcgc tggtgctctg gaacagggcg tcaaggtaag
     2821 ggaggccagg accgcggacg gataccggaa gccacaacag gaggcttcct gcaagtatca
     2881 gcagggcaaa acttcccgca aagacgcccg gcgaagccca ggacaaacgg aaacggcgac
     2941 cgaaaccggc tcggtgggga gtcatagcaa cccgaaagca gtcatctcga atggcactgc
     3001 aactggtagt cgcaacccgg gtgaagcccg tacccttaag aataccaaat tcgttacgcc
     3061 ctgcaacgct gcgtatgcgg ataggcacag gcggcacagg caacgcgcga agtagcaaca
     3121 ccccgtggaa tgactttgac cccgaaaacg atttgccggt cggttcacgc gatgttcacg
     3181 cggaacctgg ccctcctctg gatcggtcag acgatcagca tggcagggga ttccgtctac
     3241 accaaggcgc tcatatggct tttgctggac atgacaggct cctccggaac caccggcctt
     3301 gttgcagtga gcgcgtatct tccaacactc cttaccggcg tctgggcggg agcagtggtt
     3361 gaccgaagcg accacaggag agtgatgatc gcggcggatg gagtccgcgc aatgctcgtt
     3421 cttttggtcc cgattttgtt cgctgccgga gcactcacgc cttcccttct gttttgcatc
     3481 accttcctgg ttgccacggg tgcgacgttc ttcaaccctg cgcgggatgc ccttattccg
     3541 tcgcttgtat ccgaacgaac gcacctcctc cgggccaacg cgctcatcca gtcgtcgtgg
     3601 atgttcgcgc tgctcctcgg ccctgccgtg gcgatggctc tcctttcaat tctcggggct
     3661 cgcacgcagc acctgttcgt tttcggggcc gcggcatatc tcgtcagcct cgtgtgtatc
     3721 gttgctctgg acactccgcg aaagactcag gattctgcac ggggaccgtc gactctgcat
     3781 cgtgatctcg cggaaggcgt tcgctacgcg ttcaaaaacc gcgttgtgcg cggtctgctt
     3841 ctgattactg cagcggacaa cctgttcatc atggggccgg caggggtcgg catggccgtg
     3901 ttcgttcgag aggttctgaa acgagatatt ggaagcctca tggcgataga agtgtgttac
     3961 gcgatcggaa tgattgcggg tactgcactt cttccgttgt ggaacaaacg tctcggatac
     4021 gggaagattc ttatcgcagg aatggtgttg gacggggcaa ccttcgtacc cctgttgtgg
     4081 gtcgactcgt tgtggggaac aatggcgacg attgtgattc acagcctcgc aatcccgctg
     4141 ctcgtcgttc cgcggcccgc gcttgtacaa cacattgtgc cggtgaatct tcaggggcgg
     4201 gtgttcagta tgatcggtgt tactgtggta ggattcacgg ccatttccac cgggctgacg
     4261 ggtctggtga cggaatggat tcccatgccg caggtgtacg cgttcatcgg ggtggcggcc
     4321 gcgttgtgtg gtgtctgggg atacaccatg aaagacctgc gggaagccgt ttcagcgagc
     4381 agggtcagcg gttaaccatc cggttagaaa tcccgcccgc actctccccg cgtatctttg
     4441 attagcacat ctctttggca aggattctgc tcgttcaggg accacgatga tttgcggtgc
     4501 aaaaggcgcc ggttcagccc ggcaacccgg cgaagaaaca tgatgcttgg caacctgcca
     4561 ccggccagcg atccactggt gcgccgcgtg gctgaatgcg ttcggaacca ggagttgctg
     4621 gttcccggcg cgtcggttct gcttgcagtc tcaggcggcg cggatagcgt ggcgcttgct
     4681 ctcgtcttcc atgcccttgc ggcagagtgg catctttctc tccggattgc ccacatccac
     4741 catggccttc gtggcagaga agccgacggg gatgcacgct gggtggccga gttcgcggcg
     4801 gcgctggatg taccgtgcga cgtcgtccac gtcgacgtgc cggcgttgaa agaaaaggaa
     4861 cgcggctcga ttgaagacat tgccagaacg gcccgttatc gtgaactgaa acgcgtgcaa
     4921 tcagcatacg ggatggaggt tctggcaaca gctcatcatc tcgacgatca ggcggaaacc
     4981 gtcctccacc atctacttcg tggctctgga attaccggac tcggcggcat gccatggaag
     5041 acggatgaag gccacatccg tccgctcctc gaaatatcgc gcaaggagat acgtgactgg
     5101 ttgcgccgtt gggatgtgcc gtggcgggag gattcgtcca acgcctcgtt gacgttaacg
     5161 cgcaatcgta ttcgccacga gctcctcccg ttgctgcaaa aggagtggaa tcctgaagtg
     5221 tcgcgggcgc tctaccggct cgcggatgtt gcccgggcgg aagatgaagc gtttgaaaag
     5281 tgggccgaaa aggcgcttga tgatgcactt gtgtcaaaaa cccgggacct tgttctcctt
     5341 gatatccctt gtctgaagag gtacccttta ggattaaggc gaagagtgta ccgtcgcgcg
     5401 gtgcttgggc tttcgtcgat tgaagcgcct ctcagttatg ccgagacagc ggcgctcgac
     5461 agcctgcacg aacggcgcac acaggtgtcg ttccgccgcc gcttccgggc gtggagtacg
     5521 cggagtcatg ttgccttcga atcgttcgac gcctggcgcg gaccgacgga gcttcttccg
     5581 gggacacgca ccgaagttcc ctcgtggggc gcttttttgg taaccacggc agcaggctgt
     5641 ttcctttgcc ccggcaccga tttgcagtgt caggcatcgt tcagttctga cgaactcgcg
     5701 atgcctcttc atgtccgccg ttggcgtgcg ggagatcgcg tgcgatgggg tgcgtcagag
     5761 aagcttgtcg cagacctgct ccgcggaaaa aaacggaatg cagacccgag aaccgcggtt
     5821 gttgtgtgtg acagtcggcg agttgtctgg gtgcccgacc tcggcgacat tcgcgtaaca
     5881 gcgaagaagg acacgaatta cgtgaccata atccgggaat cctggcgtgt ctagtcgaga
     5941 ctctcgattg ccagaggttt tgattcccgc ggaagacatc gcgcgccgcg tcactgaact
     6001 cgggaggaag ctgtctgctg actacgccac ggtcgatgtg ggaaatgccg gcgctgagga
     6061 gcttgttgtc ctcggagttc tcaaaggtgc gttcatgttc ctcgccgatc tagtgcgggc
     6121 cgtgtccata cccatcgttg tggactttgt gcgcgcggcg agctacggtt caggccgcgt
     6181 ttcgtcgggg gatgtgcgga tcgagcccat cgcatggccg tatggcgagg gacgcgacgt
     6241 gcttgtggtg gaagacgttc tcgatacggg aaaaaccctg gacgcggtag tcagagcact
     6301 ggaaggtttc cgtccacggt cggtgaaaac cctcgcgttg ttgcgaaaac cggaggcgaa
     6361 atggcccgtc gactacgtcg gttttgaaat cgaggatcgg ttcgtcgtcg gttatggctt
     6421 ggacgacgcg ggaaaatggc gccagttgcc ctacataggg gttccccgtg atggcggtga
     6481 acggcgctga caggtaatgc caaatcggaa aagagagtgt tgatgaacgc tgagcaaccc
     6541 ggtaccaacg ataaatcacc gagaaacaca ccgcagaaca gacgaccgag aagagggggg
     6601 aacatatctc gagagcaacc gagaaatgcc ccgattcacg gctcacacca gcgtgccgcc
     6661 cgcgatgaca ggacagcgca tgctcctcag tccgcgcgcc cgtctgcggt gagtggggat
     6721 gcggaattcc ttgagcagcg caagccggaa aacccaactc cgcaggcgcg cagattcaga
     6781 aacagcgttt ccccacgaaa acggcggcgt ttccgccaac cctggactgc cggtcgaacc
     6841 gtcaccatcg taatcctgct tcttcttctg gcggtggttt tctggagaac cattgcgggt
     6901 tcccgtgcgc gcaacacgat ctcgtacacg gaattcacta cggaatacat ggcgtcgggc
     6961 aacgtgaact ccgtcagaat catcggcgac cgccttgaag cgacgctgaa aaagcctttc
     7021 ggcgaacgta agttggtgta cctgggttct ctgcagctca caccttctct gatcgattcc
     7081 tgggcccaac agggcgtcac gatcgaattc cacgccgcca caaacgtatg gggaatcatc
     7141 ctcgggaccc tgccgtacgc gacggttctc gttgttgtgt atttcctgtt tcgcaacgtg
     7201 tccgggggac agaaaggcgt tttctcgttc ggagagagtc gagcgcgcga gataaccgcg
     7261 gacatgcccg gagtaacctt cgcggaagtc gcaggtgtcg aggaagcgaa gcaggagttg
     7321 caggagatag tggagtttct gcgcgatccc cagaagttcc agcgcctggg tgggcggatc
     7381 cctcgcggtg ttctcatggt cggcccaccg ggcacgggca agacctacct tgcgcgcgcc
     7441 atcgcagggg aagcgcgggt tcccttcttc agcatcagtg gctccgattt cgtcgagatg
     7501 ttcgtgggag ttggtgcctc gcgcgtgcgt gacctttttg aaaaggcgaa aaagaaagcg
     7561 ccatgcatcg tatttatcga cgaaattgac gcggtgggaa ggcatcgggg ggctggcctc
     7621 ggtggcggac acgacgagcg cgagcagacg ctgaaccagc ttcttgtgga aatggatggg
     7681 ttcagcagtc aatccaccgt gatactgatg gcggccacga acaggcctga cgtactcgac
     7741 cccgccctgt tacgcccggg gcgattcgat cgccacatcg tggtcaatat gcccgacgtg
     7801 aggggccgtg aggggatact tcgcattcac acacgaggta tcccgctggc gcgcaatgtg
     7861 gatcttcaca ccatcgcaag gggcacgccg ggtctctcgg gggctgactt gcggaatctg
     7921 gcgaatgagg ccgcacttct tgccgcgcgg aacaaccgca gcgaagtcgg catgcgcgaa
     7981 ttcgagatcg caaaagaaaa ggtcatgtgg ggatctgaac ggcgcagcct cgtcatgtcc
     8041 gaggaggaac ggcgtgtcac tgcggtgcat gaagcaggtc acgcccttgt ggcaatctgc
     8101 catcccggcg ccgatcccgt gcacaaagtg actattgtcc cgcgcgggag gtccctcggg
     8161 cttaccgcgt ttctgcccgg cgacgagcga cacacggttt cccggcactg gtgtgatgcg
     8221 aaactcacca gtattctcgg ggggcgcgcc gccgaggaga tcgttctcgg ggacatcacc
     8281 aacggcgcgg caaacgatat cgagcttgcg accgagctgg cacggaagat ggtctgtgag
     8341 tggggaatga gcaaacttgt cgggcccgtc gcccttctcc ggaaaggcga agaggtgttc
     8401 gtcgcgaaag aactcgggcg ttccgccgag gtgagcgaac agacgctgca aattattgac
     8461 aaagaggtca accgaatcgt tgaggccgcg ctggcgaagg cgcgagacat tctggagcag
     8521 cgacgaacgg cccttgacgc gctgtccgcg gcattgcttg cgagagaggt acttgacagg
     8581 caacagatcg acgctctgat tgcccctctt ttggagccgc caagcagttc tccggtgagc
     8641 gaaagcgcgg aatcggtccc gaaggtgatt gaccgaacag gtacagatgc agtgccctcc
     8701 gcggtcagag ccgcgcgacc cgagaagggt ccggaagcac gtgtcagacg gagcaaacgg
     8761 agtgagggtt cagaagcaga acagccttca ccggtaattc agcccgaatc ggttcagccc
     8821 gagattcggg ccgaaacagc cccgcccgtc cgggaaccgg tgtctgtggc ggcgccgagc
     8881 ccgcccgtga tgtacgggag gcggcccaaa atggtcaagc gcttcgccct tccgacgtcg
     8941 caggttccgg agaacggaat tcccgttgcg agtgttgttt ctgaagctcc cattctgtac
     9001 gggagatcgt tgcgctccga aacccggacg aaggcgcagt cgcagccgct cgaggcgttc
     9061 cctcaggccc acgcttccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     9181 ggacgaaggc gcagtcgcag ccgctcgagg cgttccctca ggcccacgct tccgagggga
     9241 attcccaggg tagccgtgag cccgaagagg gaagtaccat ttcccgaacg gtttccggtg
     9301 agcagatcag cgcggctgac atggccgaga acgaccggca ggaagcgatc ggcggcgcgc
     9361 aggaaaacac tccgcagagc gcggacacga atggtgacat cgaacctggt gcggcagaca
     9421 cgctggcgaa ggttgacgag gcagtcggtt ccacctgaca aagcggacga ggcagaaaac
     9481 gttcacactc tttcatgtcg gtttcatgga catcaccgtg gctgatgtcc tcgacgttgc
     9541 tctcttaaca tttatctttt acaagagctt cgtccttttg cgcggcacgc gttctgcccc
     9601 catggtgatc gggctcatca ttctgcttgc gatcgccgtg atcgcggaac gtgcggagat
     9661 ggtcgggtcg aactggctcc tgacgcaagt gaaaaccgtc tggctcatcg ggctggtgat
     9721 tgtgtttcag acggagctgc gccggctgct tacgtacctt ggacagaaca ggctcttccg
     9781 tccattcctc gccgaggcgc cggaccagac cgtgaatgaa gtggtcgcgg catgcaagac
     9841 cctctccgag cagggaatag gggctctcat cgtcattacg cgcgacgtgg gactggccgg
     9901 aattttggag acgggtacgc gaatccgggc cgaagtgagc gcctcgctca tcgtgtcgct
     9961 gtttgttccg aagacgccgt tgcacgacgg ggcagtcgtt atttctggga accagattga
    10021 agctgccaag tgcatacttc cactgacgca gaatgcagtg gatcccagcc tcggtactcg
    10081 ccacagggcg gcgctgggag tctccgagga atcagacgcg gtggtcattg ttgtgtccga
    10141 agaaacgagc aagatatctg tcgccgttga cggcgtgctg gaacgcgcgc tgtcgccgga
    10201 agaactgcgt gcgcgtcttt tccgtgagct gaatccgcag ggcaggaaag catgagcagg
    10261 aaccatcgca tcgtgtcgac attgttttta tcccgttctt ttcaatctga tgccgccttg
    10321 tcggactgga ctgccggggt ttctgcggga aggagccgcg tcttgcccga aaggttgttc
    10381 ccctgggtat tgctcctcat tctgttcttt tcgccgcctt cccgaggcga ggtcaaagta
    10441 ctcaattcgg atgcggatgg gctggatgtg gagataaccg ctcctcttga aattgatggt
    10501 gccaatcctg cgaagttgcg aattcccgga tgggaaacca cgtcagaccc gtcccaaccg
    10561 ggcgtgccgt atcttgttgt tcccatcgtg gtgccgtcgg aaggggcagt gagcgcatcc
    10621 gtcacagcgc agaccgacac gtttgcgcgg tgcggcccgg tggaaaaggg gagtggcgat
    10681 cgtcccatcg ggcttgacga cgaggtggca ggcgccgcag cgtggtcgga cccgggcagg
    10741 cagctcgttt caatagagat tacaggcgtt gctcgcgatg tacggatggc gagactcgtc
    10801 gtctgcccgg tgagtgtagt ggaaggcggt gttcgctgga cgaagtcgct tcgcgtcgcg
    10861 gtgcgctttg cctcggagag cacgg
//
LOCUS       DAOTNG010000074        10859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5688_length_10859_cov_5.590615, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000074 DAOTNG010000000
VERSION     DAOTNG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_10859_cov_5.590615"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1073
                     /locus_tag="P5179_05165"
     CDS             396..1073
                     /locus_tag="P5179_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS94641.1"
                     /translation="MKTALTRSIVLVAVTATTLVLGGCGLSRAVKGGAIGAATGGAIG
                     AAIGNRMGSTATGAIAGAAIGGTAGAVIGHYMDRQAEEMAANLDSASVERVGEGIKLT
                     FGSGILFRSGSADLTPEASANLVQLADILKKYPDTNLIIEGHTDSDGSESFNQTLSER
                     RANTVRTALVNNGVAAERLTAVGYGESQPVMANDTPAGKAANRRVEIAVFANDKLKQE
                     AQASAAQ"
     gene            1260..2018
                     /locus_tag="P5179_05170"
     CDS             1260..2018
                     /locus_tag="P5179_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS94642.1"
                     /translation="MKNSLPKGTTLIACIGIALVTGCANLTWCGLKTAERIEPAVERV
                     LPPAPIVAPVPTAPAEPVVVPAPVTVVEAPPAPPPPPPPPPVVSVPPVVPAAPPPTEV
                     EVYMERQADEITTDLRSVATVQRTGEQIKITFGSGVLFGPGSAALTAQAQESIAKLAA
                     IIKKYPDTNLRVEGHTDSDGAEASNQRLSERRANAVAEALRSNGIPAARVTAIGYGET
                     RPVGSNDTPEGKAANRRVEITVTGGAILREGSSR"
     gene            2306..2872
                     /locus_tag="P5179_05175"
     CDS             2306..2872
                     /locus_tag="P5179_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS94643.1"
                     /translation="MRNASARVGWVFAAALSVIAAGCCGLNTAPGTARPASDATAVNA
                     YMDQQVGEMRGDLENAQVQREGQRIKVTFGSGVLFGTGSAELTPAARENISRLAGILK
                     RYPNTNVSVEGHTDSDGAEDFNQALSERRANAVAQALRDEGVAGSRIRTVGFGETQPI
                     ATNDTPEGKAANRRVEIIVMANESFGGR"
     gene            2954..4255
                     /locus_tag="P5179_05180"
     CDS             2954..4255
                     /locus_tag="P5179_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS94644.1"
                     /translation="MIYRIVAPEAGEILVSPKTGSVSLAYNGNPIDYFDRDGRLLGSQ
                     VGDRHYRRGLDNRLLEKYRRPEDGEHVRNEVPEPARQRLITAAYERAGMLADAVSRSS
                     IECRPTHPSDMALPLSAREALAGFLNPILAQGFERLCVEAMEFQRMYAPVSILPPDQY
                     LSVVAQLTTGCSYNRCTFCNFYKSRGFTVKSADEFRRHLSEVVSFPATFARLRKSVFL
                     ADGNALSLPHTKLLEMFGVLHEFFSFVPSQVCEIRERYAYRKSHPHSYEGVFSFVDGR
                     AAMEKTVDDFRELKEQHLRRVYVGLESGDDALLTFVRKPATRSEIIDAVRRMKTAGLS
                     VGVIAMVGVGGDRFAATHVEETINAINAMELGDGDIIYLSDFVNHRGLPYERLARERG
                     IRDLTSLELKTQRNGIRAGLRWKDPAKPAQVSLYDIREFIY"
     gene            complement(4258..5007)
                     /locus_tag="P5179_05185"
     CDS             complement(4258..5007)
                     /locus_tag="P5179_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS94645.1"
                     /translation="MRLTRSLYVVGGHCVGLSDTQDCIIYVVAAPEGLIMVDAGGGRD
                     TDAIFRAMAEEGLDERQLRVLLLTHCHADHAAGAAAIRERTGCAVAISERSAHLLENG
                     TEIDVRLDLAKAMRIHPPDLVWANCPVDIRLSDGQLLDILGITVHVFEVAGHSEDSCC
                     FVMEVDGRKCLFAGDVIQYGGVIGLLNYPGSTLDGYRRDLPKLRGLGIEALLPAHSLF
                     TMRNGQHHIDSAIERLKSHIVPPTVGQKVFF"
     gene            5239..7491
                     /locus_tag="P5179_05190"
     CDS             5239..7491
                     /locus_tag="P5179_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRS94646.1"
                     /translation="MSFLDQLNPEQRRAAQILSGPVLILAGAGSGKTRTLTHRLAHLI
                     ASGTPAHRILAVTFTNKAAKEMKERTARLIGEARAASLWIGTFHSICARILRRDAHLL
                     GYDARFTIYDSDDSLTLVKRIMRDHGTLDDSLKPGIVRARISEAKAFLLTPEEYARNR
                     KGPAAAVIADLYRRYQAALISNNAMDFDDLLALPVRLFRTQPEVLEEYRNRFQHLLID
                     EYQDTNQAQYVLVKMLAESHHNICAVGDDDQSIYGWRGADITNILNFENDFPDATVVR
                     LEQNYRSTRTILKAAGAVVRNNRTRKVKELWTQNDPGDHLTIAELADEKDEAEWIRER
                     LVRLLEDGHSYGDIAVLYRTNAQSRAIEEAFVRSSKPIPYTVVGGLRFYERKEIKDVL
                     AYLRVIDNPRDSESLRRIVNVPRRGIGEKTMALAAERMGATGLPLLDALRTLEEHELG
                     PRAYKPINEFIRWLDALIANKGEMAVDELLEKILTDTGYRAQLEQDGSVEAETRLQNI
                     GELVAGATAYVEANNEPNVSAFLQDIALVTDADEVQTDGGSSVTLMTLHSAKGLEFPV
                     VFVSGMEEGLFPIAGSLQDSAELEEERRLFYVGLTRAQKLVFLSYAQRRRRFNEWANT
                     QPSRFLREIPDELLKWDTRSTEEPGRRILDQRRFREDGSVGVRGISTRRIHAVGDEFS
                     QESSYADDFSQTTDSFLAIGHHVMHPMFGRGRITAREGLGLDTKLTIHFDSGQTKKIL
                     VRVAHLEPCV"
     gene            complement(7553..8803)
                     /locus_tag="P5179_05195"
     CDS             complement(7553..8803)
                     /locus_tag="P5179_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS94647.1"
                     /translation="MNAKPVLTIARREYIARVRTKSFVVMTVLIPLIMGASVAAPMFL
                     VKAESPRQRVLAVADETGLVFDSLRSLREETLPNGKPRYLIERVAATPRAVSDARRLV
                     ELEAYDAFAFVPAGAVDGLPVTYYTNGVPNPQEVDHVRGAFNRVLIAGRLAREGLDPA
                     RVSALTRSVRFDVVALQKGAAPKTAQAAFAAAVALAILMYISLTMYGAFTMQSVVNDK
                     ASRVVEILVSTVSPTELMYGKILGTGCAGLTQLGIWMAVILGVSIGMPESALAKTFHA
                     VAGVSIPALILFFVTGFFLYASLYAGVGAICTTNEEAGQLQLPVSLLLMVAFFLAVSG
                     VNDPGSTLITVLSYVPFFAPVLMMLRFGVGEASWFDAAVSLAGVVLTTAIIAAAVGKI
                     FRVGILMTGKRASLTEVLRWLRRA"
     gene            complement(8800..9750)
                     /locus_tag="P5179_05200"
     CDS             complement(8800..9750)
                     /locus_tag="P5179_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS94648.1"
                     /translation="MKPPIDRAWCGMTGLPLVLENVSKSFGPTRAVDNLSLALRAGSV
                     CGLLGPNGSGKTTTIRMIMGILLPDSGDLRLWGAPAEGAVRKHVGYLPEERGLYATMT
                     VFDHLIFFAELHDLSRRDAKDAVAEWSERFATNGESSPKSWWGRRVQELSKGQQQTVQ
                     LIGTLLHRPGLVILDEPFSGLDPVHTGHLRTIIRYLKESGVAVLLSTHRMEQAERLCD
                     DICLVHHGKAVLSGALREVKSASTSRLVELRMDRVPDFLEPDDMIADVEIVSNGARIR
                     LREGADAQMVLDRAIRRGRVLKFEIVEPSLDDLFIEAVSR"
     gene            complement(9747..>10859)
                     /locus_tag="P5179_05205"
     CDS             complement(9747..>10859)
                     /locus_tag="P5179_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS94649.1"
                     /translation="GIDTVYVLTQFNSVSLHRHIHRTYRFDVFGRGFVEILAAEQTME
                     NTGWFQGTADAVRRSLRYIDLPDYKHILILSGDHLYRMDYREFVATHLRSNNDVTVSV
                     KPVGLEETSGFGIVRMDESREIIEFLEKPTEPADLERLRLPDAPPGRNFLASMGIYVF
                     RREVLVDLLNRGTEEDFGRQVIPRAIAETRVGAHLFDGYWQDIGTIGSFHRANIDLAA
                     ELPPFNFYEPGAPIYTHPRFLPSSKINNAHVQQSMLSEGSIVMEADIQQSIIGIRSII
                     QRHAVIRKSVIMGADFYETDRDRAHNRSIGRPDVGVGEKCLIENAIVDKNARIGDGVQ
                     IINAAKINNARHADYVIADGIVVIPKDAVIPPGTVI"
BASE COUNT         2399 a         3194 c         3173 g         2093 t
ORIGIN      
        1 cgcgtagtag tccaaccagc gcgaccttgg caacccggaa ggatagaaca ctccctgcca
       61 gtcgtcgaaa tgaaaacccg aggttcccac ccagatacgt gtgggagttt cttcagagtg
      121 aaccttttcc acacctgttc cgctcatctt gcctggcaat ttgcgaatcg tttctcgaca
      181 aaactgctca aggtgtttac agaaagatca aacccgcata tatttcttgg caacggagca
      241 ggcaacgcgt gcttttgtcc gggacgtcac ggcgtaccac cgtcccggtt ccctacgcgg
      301 ttggcagacg ccccgctgcc ctttcgccca ccatacaaac gtatggggcg tggtctgcgg
      361 ggaaatgtct catttccaga aagaggagaa tcggaatgaa aacagctctt acccgtagta
      421 tcgttcttgt cgctgtgacc gcgaccaccc tcgtgctggg cggttgcggt ttgagcaggg
      481 ctgtcaaggg cggcgcgatt ggcgcagcca caggtggcgc gattggagct gccatcggta
      541 accgcatggg cagcaccgcc accggagcga tcgccggcgc ggctattgga ggaaccgcag
      601 gcgccgtgat tggtcactac atggaccgac aagcggagga gatggcggca aatctggata
      661 gcgcaagtgt cgagcgcgtc ggagaaggga taaagctcac attcggttcg ggtattcttt
      721 tccgatccgg ttccgcggat ctgactccgg aagcgagcgc gaatctcgtg caactcgcag
      781 acattctcaa gaagtatcct gacacaaatc tgattatcga aggtcacacg gattcggatg
      841 gctctgagtc gttcaatcag acactgtccg agcggcgcgc gaacacggtt cgcactgcgc
      901 tcgtcaacaa tggcgtggct gcggagcgct tgaccgcagt ggggtatggc gagagccagc
      961 cggtgatggc taacgataca cctgccggga aagccgcgaa ccggcgcgtt gaaatcgccg
     1021 tttttgcgaa tgacaaattg aaacaggaag ctcaggcgag cgcagcccag taacctgtgc
     1081 gattcgtgat ctgacgcccc ggtggggaaa ccctccgggg cgtcgctttt ctgagcagaa
     1141 atgggcacgg gtttccagat ctgaagcgtt gccggaggca tgtcggcaca gaagggatcc
     1201 ctctgcgcag tgtttttcgg gggagatcga aatccgcgca tcgcaagaag gacgttgccg
     1261 tgaaaaactc gctaccaaag ggcaccacgc tgatcgcgtg tatcggcatc gcgttagtaa
     1321 cgggttgcgc caacctgacc tggtgcggtc tgaagacggc cgagcgcata gaaccggcgg
     1381 tagaacgtgt cttgcctcct gctccgattg tggcaccagt tccgacagcg ccggcggaac
     1441 cggttgtggt accggcacct gtcaccgttg ttgaagcgcc tcccgcaccg ccacccccgc
     1501 cacctccccc accggtcgta tcggtaccac cggtcgtacc ggcagcgccg ccacctacgg
     1561 aggtggaggt gtacatggag cggcaggcgg acgaaataac caccgacctg cggagcgtcg
     1621 ccacggttca gcgcaccgga gaacagatca aaatcacatt tggctcggga gtccttttcg
     1681 gacccgggtc ggccgcgctg acggcccaag cgcaggagag tatcgccaaa ctggccgcga
     1741 tcatcaaaaa gtaccccgac acgaatcttc gggtcgaagg tcacacggac tccgatggtg
     1801 ccgaggcctc caatcagcgg ctgtcggaac gacgagcaaa tgccgttgcg gaagctctgc
     1861 ggagcaatgg catcccggct gccagggtta cggcgatagg gtacggtgaa acgcggcctg
     1921 tcggatccaa cgacactccg gagggcaagg cggctaatcg acgagttgag atcaccgtca
     1981 ccggcggcgc aatactgaga gagggatcat cgagataacg ctccgatcgg attcatctga
     2041 cgcctcgacg atgtccctcg tcggggcgtt ctttgtgtgg ccattgacgc aatgagtttg
     2101 ctacagcctc tttcgctgtc tatatttccc tcgcgatgcg agacttttgt tttgcttttg
     2161 cagttgtacc cagaagctta cgcgatctga atctttctga gacgtttccg gtcggtgcgt
     2221 gtcgccgacg tttccagcaa cggattcccg cgggctgatc aaatcgccgt tagcggggat
     2281 ctcactcgcc gaaaggagaa cctgtatgag aaacgcatct gcacgtgtcg gatgggtttt
     2341 cgcggctgct ctgagcgtga ttgcagccgg atgctgcggg ttgaacaccg cccccggaac
     2401 cgcacgacct gcgtcagacg caaccgcggt gaacgcttac atggatcagc aggtcggcga
     2461 gatgcggggg gaccttgaga acgcccaggt tcagcgcgaa gggcagagaa tcaaggtcac
     2521 ctttgggtcc ggtgtgctgt ttggaacagg atccgccgag cttactcccg ccgcgaggga
     2581 gaacatttcg cgcctggcag gaattttgaa gaggtacccc aatacgaacg tgagcgttga
     2641 aggtcacacg gactcggatg gcgctgagga cttcaaccag gcgctttcgg agcggcgcgc
     2701 gaatgcggtg gcgcaggcgc tgcgtgacga aggtgtggcc gggtcgcgca ttcggacggt
     2761 tggattcggg gaaactcaac cgatcgccac gaacgacacg cccgagggga aagccgccaa
     2821 ccgccgggtt gagatcatcg tgatggcaaa cgaaagtttc gggggacgct aagcgttctg
     2881 aaccacgtgc ctaacgcgaa gtgcgccccg acggacgaat gctgtcgggg cgcgttgctt
     2941 ttttcattga cagatgatct acagaatagt tgccccggaa gcgggagaaa ttcttgtcag
     3001 ccccaaaacg gggtcggtgt cactcgcgta caacggcaat cccatagact atttcgatcg
     3061 tgatggacgt ctgctcggaa gccaggtcgg agaccggcac taccgccggg gtttggacaa
     3121 caggcttctt gagaaatacc gacgaccgga agacggggaa cacgtcagga atgaggttcc
     3181 ggaacctgcg cgccaacgat tgatcacggc agcgtacgag cgggcgggaa tgcttgcgga
     3241 cgcagtgagc cgaagctcca tcgaatgcag accgacacac ccgagtgata tggcgctgcc
     3301 gctttcggct cgagaggctc tcgcaggttt tctgaacccc atcctggcgc agggtttcga
     3361 acgactgtgt gtcgaggcaa tggagttcca acgtatgtac gctcctgtca gcattctgcc
     3421 ccccgatcag tatttgagcg tggttgcgca gttgaccacc ggttgctcct acaaccggtg
     3481 cacattctgc aacttctaca aatcacgggg gttcaccgtc aaatcagcgg atgagtttcg
     3541 ccgccatctg agtgaagttg tctcgtttcc ggccaccttc gcccgtctcc ggaaatcggt
     3601 gttcctcgcg gacgggaacg cgctcagcct gccgcacacg aagctgctgg aaatgtttgg
     3661 cgtgctgcac gagttctttt cgttcgttcc ttcgcaggtg tgtgagatac gcgagcggta
     3721 tgcgtacagg aagagccatc ctcactcata tgaaggagtc ttcagtttcg tggacggtcg
     3781 cgccgcgatg gaaaaaaccg tggatgactt ccgcgagctt aaggaacagc atctccgcag
     3841 ggtttacgtc gggctggaaa gcggggacga cgctctgctg acgttcgttc gcaaaccggc
     3901 aacgcgctca gagatcatag acgcggtccg ccgcatgaaa acagccggat tgagcgtggg
     3961 agtaattgcg atggtcgggg tgggtggaga cagattcgcc gccacgcacg tggaggagac
     4021 tatcaacgca atcaacgcga tggaactcgg cgatggcgac atcatctatc tgagcgactt
     4081 cgtgaaccac cgcggactgc cttacgagcg gctggcgcgg gaacgcggaa tcagagacct
     4141 caccagcctg gaactgaaga cgcaacggaa cggaattcgg gcgggcctac gctggaagga
     4201 tccggccaaa cccgctcaag tctctcttta cgacattcgt gagttcatct actgatttca
     4261 gaagaagacc ttctgcccga cagtcggagg aacgatgtgg gatttcagcc gctcaattgc
     4321 tgaatcaatg tggtgttggc cgttccgcat cgtgaacagc gaatgtgccg gcaacagcgc
     4381 ctcaatcccg agtccgcgca acttcggaag atccctgcgg tatccgtcga gggtgctacc
     4441 cgggtagttg agcagcccga tcaccccgcc gtactgaatc acgtccccgg cgaacaggca
     4501 tttccggcca tccacttcca tcacgaagca acacgaatcc tcgctgtggc cagcgacttc
     4561 gaacacatga acagtgatgc cgaggatgtc cagcagctgc ccgtcggaaa ggcgaatgtc
     4621 caccgggcag ttcgcccaca cgaggtccgg cggatggatg cgcatggctt tggcaaggtc
     4681 cagacgcaca tcgatctccg tcccgttttc cagcaggtgg gcagatctct ccgaaattgc
     4741 caccgcacat ccggtacgtt ccctgatcgc cgcagctccc gcagcgtggt cggcgtggca
     4801 gtgcgtcaga aggagaacgc ggagttggcg ctcatccagc ccctcctccg ccatggcgcg
     4861 aaaaatcgca tccgtatcgc gcccgccgcc cgcatccacc ataatcaggc cctcgggagc
     4921 cgccacaacg tatatgatgc aatcctgcgt atcggagaga cctacacagt gaccgccaac
     4981 aacgtaaaga gagcgggtca atctcatgga acacaccccc aaattcggcg ttcgtcgacg
     5041 ttacgcagcc tgtgaaagta atatcccgtt cagttgcggc gacgccaaca cctgcgtgca
     5101 gaacggtggg cccgcgcggc aagacagaaa cgcccgcggc cttcgtatct ttggaaacga
     5161 aatgattcgc ccggatcgct cgatcctgcc ggacagtgca cccacgtacg atctcgaaaa
     5221 cctgaggacc ccagtttcat gtcgtttctc gaccagctca atccagagca aagaagggcc
     5281 gcgcagattc tttcaggccc cgtcctgatt ctcgccgggg cgggcagcgg gaaaacccgg
     5341 accctcacac accggcttgc acacctgatc gccagcggca cccctgcaca cagaattctc
     5401 gcggtaacgt tcacgaacaa ggccgcgaaa gaaatgaaag agcgtactgc ccggttgatc
     5461 ggtgaggccc gagccgcgtc gctgtggatc ggcacgttcc actccatctg tgccagaata
     5521 ctgcgtcgtg acgcacatct actcggctat gacgcgagat tcacgattta tgacagcgat
     5581 gattcgttga cacttgtgaa gcgcatcatg cgggatcacg ggacgctgga tgacagcctc
     5641 aaaccgggga tagtgcgtgc gcgaatatcg gaggcaaagg cctttcttct cacacccgaa
     5701 gagtacgccc ggaaccggaa aggacccgcc gccgcagtga tcgccgatct ctaccgacgt
     5761 tatcaggccg cgctgatttc caacaacgca atggattttg atgacctgct cgcgcttccg
     5821 gttcgtcttt tccgcacaca acctgaggta ctcgaggaat accgaaaccg gtttcaacat
     5881 cttctcattg acgaatatca ggacacgaac caggctcagt acgttcttgt caaaatgctt
     5941 gccgagagcc accacaacat ctgcgcagta ggcgatgatg accagtcgat ttacggatgg
     6001 cgaggcgccg acatcacgaa catcctcaac ttcgaaaacg actttcccga cgccacggtt
     6061 gtgagattgg agcagaatta ccgttccacg cggacgattc tcaaagccgc gggggcagta
     6121 gtgcgcaaca accgaacgcg gaaggtgaag gagctctgga cacagaacga tccaggcgat
     6181 cacctgacaa tcgccgagct tgctgacgag aaggatgagg cggagtggat tcgcgagcgt
     6241 ctggttcgac ttctggagga tggccactct tacggagaca ttgcggtcct ctatcgcaca
     6301 aacgcccaat cacgagcgat tgaggaggcg tttgttcgat cttccaaacc catcccgtac
     6361 acggtggtcg gtggtctccg cttctacgaa cgaaaggaaa tcaaggacgt tctcgcgtat
     6421 ctgcgggtga tcgacaatcc acgtgactct gagagcctca ggcgcatcgt gaacgtcccg
     6481 cggcgcggca tcggcgagaa aactatggcg ttggccgcag aacggatggg agcaacgggt
     6541 ctgcccctcc tggacgcgct gcgaaccctg gaggagcacg aactcggccc ccgcgcgtac
     6601 aaaccgatca acgagttcat acgatggctc gacgcactga tcgcgaacaa aggcgaaatg
     6661 gcagtagatg agcttcttga gaagattctg acggacacgg gataccgtgc gcaactggaa
     6721 caagacggat ccgttgaggc cgaaactcgc ctccagaaca tcggggaact ggttgcaggc
     6781 gctaccgcgt acgtggaagc gaacaacgaa cccaatgtca gcgcgttcct ccaggatatc
     6841 gcgctggtaa ccgacgcgga cgaggtgcag acggatggag gcagttcggt taccctgatg
     6901 acgctccatt ccgccaaggg tctcgaattc ccggtagtat ttgtgtccgg catggaggag
     6961 gggctcttcc cgatcgcagg gtctttgcag gactcagcgg aattggagga ggagcggcgt
     7021 ctgttctatg ttggcctcac tcgcgctcag aagctggtgt tcctgagcta cgcgcaacgc
     7081 cgccggcgtt tcaacgagtg ggcaaacact cagccttcgc gattcctgcg ggaaatcccg
     7141 gatgaattgt tgaagtggga cacgagaagc accgaggaac ccggaaggcg tatcctggac
     7201 cagcggcggt tccgagaaga cggatctgtc ggcgtgcgag ggatctcaac tcgtcgcatc
     7261 cacgcggttg gggatgaatt ttctcaggaa tcgagttatg ccgacgactt ctcccagaca
     7321 acggacagtt tcctcgccat cggacaccat gtgatgcacc cgatgttcgg gcgtgggaga
     7381 ataacggccc gcgaaggcct cggactggat accaagctga cgatccactt cgacagcgga
     7441 cagacgaaga agatcctagt tcgtgtcgcc catctggagc cttgcgtcta ggactgagtg
     7501 gggtacctga cgcgacgctt gctgccctcg gcatcctctg accggaaatc agtcacgctc
     7561 tccgcagcca gcggagcacc tccgtcagcg acgctcgttt ccccgtcatg agtatgccca
     7621 cccggaaaat cttccccacg gcggccgcga taatggccgt tgtgaggaca acccccgcca
     7681 gtgagacggc ggcgtcgaac caacttgcct ctccaacgcc gaaacgcaac atcatgagaa
     7741 caggagcgaa aaacggcacg tacgacagca ccgtgatcaa ggtgctaccg gggtcgttca
     7801 cgccggacac cgccaggaag aacgcaacca tcagaagaag cgacacgggc aactggagtt
     7861 gccccgcctc ttcgttcgtc gtgcagatcg caccgactcc ggcgtagagc gaagcataca
     7921 ggaaaaagcc ggtcacgaaa aaaaggatga gggcggggat cgagacaccc gccacggcgt
     7981 ggaacgtctt cgcgagcgca ctctcgggca tgccaattga gacacccaga atcacagcca
     8041 tccagatgcc caattgcgtg agcccggcac aaccggtgcc aaggatcttc ccgtacatca
     8101 actccgtggg cgataccgtt gacacgagga tctcgacaac ccgcgatgct ttatcgttca
     8161 caacactctg catcgtgaac gcgccgtaca tcgtcaagct gatgtacatc aggatcgcca
     8221 atgccacagc ggccgcgaaa gctgcctggg cggtttttgg cgcggctcct ttctgcaacg
     8281 caacaacgtc gaaccggacg gaccgtgtca gggcggagac acgtgccgga tcgagcccct
     8341 cccgcgcaag cctgccggca atcagtaccc tgttgaaggc acccctgaca tggtctactt
     8401 cctgggggtt cggcacaccg ttggtgtaat atgttaccgg gagaccgtcg acggcccctg
     8461 ccggtacgaa ggcaaacgcg tcgtaggctt ccagctccac cagcctgcgc gcatcagaca
     8521 ctgcacgagg agtcgccgct acccgttcga ttagataacg cggtttgccg ttcggcaggg
     8581 tttcctcccg cagcgaccgg agtgagtcga atacgagccc ggtttcgtcc gcaaccgcga
     8641 gcacccgctg tcggggagat tccgctttca caagaaacat cggggccgct acgcttgcac
     8701 ccatgatcag ggggatgaga accgtcatga cgacaaaact tttcgttctg acgcgtgcga
     8761 tgtactcacg ccgcgcgatg gtgagcacgg gttttgcgtt catcggctca ccgcctcgat
     8821 gaagaggtca tccagggaag gctccactat ctcgaatttc agcacgcgcc ctcgccgaat
     8881 cgcgcggtca agcaccatct gcgcatccgc accctcccga agacggatcc gcgcgccatt
     8941 ggagactatt tcgacgtcag cgatcatatc atcgggttcc agaaagtcgg gaacacggtc
     9001 catacgcagt tccaccaatc ggctcgtgga ggcggatttc acctcacgca gtgcacccga
     9061 caacaccgcc ttcccatggt ggacgaggca tatgtcgtca caaagccgct ctgcttgttc
     9121 catgcggtga gtggaaagga gaaccgcgac gccagactcc ttcaggtagc gtatgatcgt
     9181 ccgcagatgg cccgtgtgca cggggtcgag cccactgaag ggctcatcaa ggatgacaag
     9241 tccggggcga tggagaagcg ttccgatgag ttggacagtc tgctgctgcc ctttggatag
     9301 ctcctgtacg cggcgccccc accacgactt cggcgacgac tcaccattcg tcgcaaacct
     9361 ttcggaccat tctgcgacag cgtcttttgc gtctcggcgc gataggtcgt gcaattcggc
     9421 aaagaaaatc agatggtcga aaacagtcat ggttgcgtag aggccgcgct cctccggcag
     9481 gtatcccacg tgtttccgaa ccgcgccctc ggcgggagct ccccagagtc gcaaatcccc
     9541 tgaatcgggc aacaggatac ccatgatcat ccgaatggtg gtggttttcc ctgacccgtt
     9601 cggaccaagt aatccgcaca cagatccagc ccgaagagca agcgaaagat tgtcaacggc
     9661 gcgggttggt ccaaaagact tcgatacgtt ttccaagacg agaggcaggc cagtcatccc
     9721 acaccacgct ctgtcaatcg gtggcttcaa atcacggttc ccggtggaat caccgcgtcc
     9781 ttaggaatca cgacgatacc gtcggcaata acgtaatcgg cgtggcgcgc gttgttgatc
     9841 ttcgccgcat tgatgatctg cactccgtcc ccgatgcggg cattcttgtc cacaatggcg
     9901 ttttcgataa gacatttctc gccaacgcca acgtccggac gcccgataga ccgattgtgg
     9961 gcgcggtccc tgtcagtttc gtagaaatcg gcccccatga tgactgattt ccgtatgacc
    10021 gcgtgcctct ggatgatact ccgaataccg ataatgctct gctgaatgtc cgcttccatc
    10081 actatcgaac cttcgctcaa catgctctgt tggacgtgcg cattgttgat cttggagctg
    10141 ggaaggaatc gcggatgagt gtaaatgggc gcccccggct cgtagaaatt gaagggtgga
    10201 agctccgccg caagatcaat gttggctcga tgaaatgaac cgatcgtgcc gatatcctgc
    10261 cagtacccgt cgaaaaggtg cgcgccaacc cgcgtctccg cgattgcgcg aggaatcacc
    10321 tgtcggccga aatcctcctc cgttccgcgg ttcaggagat ccaccagaac ctcccgacgg
    10381 aacacgtaga tccccatcga cgccaggaaa ttcctccccg gaggtgcatc aggaaggcgc
    10441 aatcgttcaa gatccgccgg ttccgtcggc ttctccagaa actcgatgat ttcgcggctt
    10501 tcgtccatcc gaacgattcc gaaaccggac gtctcctcga gaccgacagg tttgacggaa
    10561 accgtcacgt cgttgttgct ccggaggtgc gtggcaacaa actcccggta atccatgcga
    10621 tacaaatggt cgccggaaag aatgaggatg tgtttgtagt cgggcaaatc gatgtagcga
    10681 aggcttcgtc gcaccgcatc tgcggtaccc tggaaccagc ccgtgttctc catcgtctgc
    10741 tcagccgcga ggatttccac gaaccccctt ccgaacacat cgaaccggta ggtgcggtgg
    10801 atgtgtctgt gcagggaaac gctgttgaat tgcgtgagga cgtagaccgt gtcaatccc
//
LOCUS       DAOTNG010000075        10822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5712_length_10822_cov_4.430854, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000075 DAOTNG010000000
VERSION     DAOTNG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_10822_cov_4.430854"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="P5179_05210"
     CDS             <1..203
                     /locus_tag="P5179_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRS94650.1"
                     /translation="IVDRLEAEDGDFHERVRNGFLEIARREPDRVAVLDGTLPVDDVT
                     NAIVDTLALRVPVLLGDVIKSS"
     gene            294..2114
                     /locus_tag="P5179_05215"
     CDS             294..2114
                     /locus_tag="P5179_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS94651.1"
                     /translation="MALRRRDWGWLALMVAVVIWGGQIFNHVEALAQDAYGRIGQMAD
                     DRAEVETAILRFYVEDVDAGKLQLSQVDGMLNELDTYSDFLNEREQEDLRMTTEGAFG
                     GLGIEINAVDHYPTVISPLEGTPAWDAGLLAGDQIIEIENESTYDVKLDIIVGKLRGV
                     PGTAVNIKVRRPGSSEPIDFRITRAEIHVNSVQFHGLLWDPRAEGFLPLGAPDWLRNA
                     QGIGYIRLSSFSRGATDEVRVALQDLMAKRARGIVLDLRMNPGGLLEEARGVADLFLP
                     KGRLIVSTKGRAQRGEQRLYSERDPAIPPSMPLVVLVDEGSASASEIVAAAIQDNDRG
                     LIVGRPTFGKGSVQTVYNSQFSGRFGLSFSQNALLKLTTAYYYSPSGRCIHKPRWRAG
                     KRGAVVSKAASDTATVYRTSKGREVRGGGGVAVDVPVQPDVPPALFWQLNARQLFFNY
                     AVDYAVKHPELDPQTFDVNEAMLSDFRRFVADSVHAFTYEPRGKKELDDFAKATSEAG
                     YSQEVTRRVEELREALLAQKDAEFSRAEPYIRQRLRSAIAGRVWGRQSRTRADFTRDL
                     ALKEALTYLTDSGRYAQQIALSPVRPRPQQQEQAAENEGD"
     gene            2236..2841
                     /locus_tag="P5179_05220"
     CDS             2236..2841
                     /locus_tag="P5179_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS94652.1"
                     /translation="MELANQSDSELISMFLDGQAAAFDTLVRRYERQVFGFIFGMVQN
                     EALANDLFQETFIRIINALPDYRERGKFASWLLGIARNLALDALRKRRVEGRIFRLEN
                     RTTDSGNAGSTYPEIPDDIESQADMVEREEWLVRMREALAELPAEQREVVYLRYEADL
                     TFQQIADLTGVSINTALGRMRYALNSLRKRMNVATEPSTNE"
     gene            2834..3460
                     /locus_tag="P5179_05225"
     CDS             2834..3460
                     /locus_tag="P5179_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRS94653.1"
                     /translation="MNDTNGTPACLRTQRDALRYTSGELSAEERITFEVHLQHCRECA
                     RLVDDERTIRRVFGALPRSSPSATVRALISERAAASGAGYHKLGSPGRIAPLRTALVT
                     RIPVWTWPVAAAAILLLGVSLTFHRDVRTPVFPSDTLSAPVALVPDVPAGDGIQVASV
                     SETTKASTRIRTPRDGASASDNLLDDDVVELADRVETLAMILDEETTQ"
     gene            3634..4092
                     /locus_tag="P5179_05230"
     CDS             3634..4092
                     /locus_tag="P5179_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94654.1"
                     /translation="MALEFIRRIDSSRVEQVIALKTSRPHAYRSMILRSLNMERHARA
                     MGRRGDPGGIQQSEQLFRRMFELREQMDRLSIQYRLATDENAKNEIRARFSAKAGELF
                     DVQTEMNRLQIEQIADELRRLQQRLEQRQAGRDAHIRRQVEIMLSDEELD"
     gene            complement(4064..4615)
                     /locus_tag="P5179_05235"
     CDS             complement(4064..4615)
                     /locus_tag="P5179_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HRS94655.1"
                     /translation="MTEALHLPPLPPCAADMMLGRLATWLRLLGADVSYQSWISDADL
                     MERARNENRLVLTRDRGLLGLPGSTPRFGVTFDRLPDQLAQVAAHFDLTALVPFSRCV
                     RCNVPVKVIPKESVKGRVWEYVYDTQEIFTECPLCGRLFWKGTHPARAREDLRRMLGE
                     DLARRIRWGEPDDGFSQAPHRKA"
     gene            4753..5778
                     /locus_tag="P5179_05240"
     CDS             4753..5778
                     /locus_tag="P5179_05240"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS94656.1"
                     /translation="MKEWDRRIAHPITGQENPAYLHGVIVPMFTPCNSNGTLDERGIR
                     SYVNYLVSNGSVNALFARSGLGRMYTFSVNDVRRLADIVIDEVADRLPVFVGCSGELS
                     ADSPERPDAHTYLRQSIELCQYVQRKGATAAVLVLPVALVPHREELPAEVVLEYFRRV
                     EAETDIPLIIYQPPETTRRFRATPSLIEEVSRSGQVVGMKISTSRMDVFLSCSKAAQN
                     ANFALIAGDETVFLFTILVGGTGVIGQGCTTHPEILKAVYERAMAFDVAGASKAALDT
                     FAAVNAAPGEDPRRCGLPIPPEGRGDRTALFKGWGPSTFGGNKRTLCLQSGRSEVRVP
                     RFNGPLV"
     gene            5806..6852
                     /locus_tag="P5179_05245"
     CDS             5806..6852
                     /locus_tag="P5179_05245"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS94657.1"
                     /translation="MKRWTDPVVQPYTGRAIPAYLHGVITPMFTPCNSDGSLDEKGIR
                     AFVDYLIGTGCITTLFARSGLGRMYSFSYEDVKWFADVVLDQAAGRIPVMIGTTGIWG
                     RDFHHRPGNEIFTRQSIELSQYVQKKGAVAAVLVLPMALQPEEGRPLADTIFAYYEAV
                     AREVDLPIILYQQPGLLEEYCMTPPLLERLLTIKNIAGMKYSTGDMVKFSELTMVARN
                     TDFAFIAGDELAFFFAMMLGASGVIGQGCDVNPETLRAVYDRMMARDFAGALEAHYDC
                     IRAGQISEGYDCVISGFRYVAGKGVRVQPFTRGHGVSDYQVQQPVVPDDWMASFSREM
                     DALRRKYPPVNPWA"
     gene            complement(6910..7836)
                     /locus_tag="P5179_05250"
     CDS             complement(6910..7836)
                     /locus_tag="P5179_05250"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRS94658.1"
                     /translation="MTGTTLSPDLRTSVGALDLANPVLVASGTFAYGREMARLYDLSL
                     LGGIVSKTITELPRAGNPPPRVCETVGGMLNSIGLPNPGVDAFLRDALPELRQAACPV
                     IVNVAGETAEGYVRLAARVAREKGVSAVELNVSCPNVSHGLDFGTAPSLVEQLVRRVK
                     QECALPVIAKLSPNVMDIAEIARGVEQGGGDAVSLINTIRGMAIDARTRKPVLGNVTG
                     GLSGPAVKPVALAMVWKTSRSVRIPVIGIGGIMTGTDVVEFMLAGASAVQIGTGNFVD
                     PWVAPRVISELRDFCIQEDIASVRSLIGELKA"
     gene            8041..9294
                     /locus_tag="P5179_05255"
     CDS             8041..9294
                     /locus_tag="P5179_05255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS94659.1"
                     /translation="MDAKKKARVFVQTAAAPLSELDRWVQVGEEEARAVYEMTLQNEL
                     SGGTEQVRAFENEFREFTGLKHAISVINGTAAIWSAMYGCYVGPGDEVICPVYTWLCS
                     ITPAPMLGARPVFCDVSPHTLLMDPEDVKKKITSRTRAIVAVHLWGMVCDMQALGAIS
                     QETGIPIIEDCSHAHGALFRGRVVGATGKVGAWSMQGSKAVSAGEGGVLATDDDEVFD
                     RACMIGQVNRAAGLDLVSAKYQKYQPLGLGIKFRAHPLGIGIARVQLRKLGLLNAGRG
                     GYIEAVERALKEIPGLDPVTLTDGTKRGGYYGFPTIFRAEQVPGVTRDQYVNALRAQG
                     VPASSSPYDLLHRLPYFAEGMDVFGFGRGVMCEGYNGYKEGDFPGAEEMYRNVVFLPV
                     LTDPVEGATEEIIRRIRKGVESLGF"
     gene            9284..10468
                     /locus_tag="P5179_05260"
     CDS             9284..10468
                     /locus_tag="P5179_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRS94660.1"
                     /translation="MDSKDHAATPPRSEDSWIVFSSRRTEYACAEIGFVRPDGTGLHF
                     PRLDIEGQVSWHAGPAFADGDRIILHSVENSETWKHRSRSNLWIFRISSGELTALPLK
                     HRPADFMPPALLLPGDERMVVNPVIQGNQCVVTMNLDGSDPVHVTKPEDGFAYGIALS
                     PDCSMLAFHIAGPPNYFACVGDIDGANRRVVARDSDVLFFAPMWSRNGDWLVCEGCFS
                     GKDPEHFVADLYLVRPDGRELHPVTSGQSHWFAAAYGDEKSRSGGSNVSRWSPTEDVV
                     SYTRLAPGSRTAWKHIEQPQEDDHFNRAYAPEEARGGTQLCALNPFERACRPLTEVRE
                     GVWDMRAAWSSDGALIAFCRAHVGQPPGLWVMSADGGQARLLTVGKEGLGADHPAWIH
                     AR"
     gene            10525..>10822
                     /locus_tag="P5179_05265"
     CDS             10525..>10822
                     /locus_tag="P5179_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS94661.1"
                     /translation="MEPQESGRSPVNWYTADPWKAYLGWLSPRWAEPREDFDPEEWCG
                     ALVEGGFRTAVVHAKHHDGTCFFPSRFRSAQPPRDFFGEIVGAARKLGIRIVAYY"
BASE COUNT         2252 a         2963 c         3383 g         2224 t
ORIGIN      
        1 agatcgtgga ccgcctcgag gctgaagacg gcgatttcca cgaacgtgtc aggaacggct
       61 ttctggaaat agcacggcga gaaccggatc gggtagcggt tcttgatgga accttgcccg
      121 ttgacgatgt tacgaacgcg atcgtggata cccttgcgct gcgagtacca gttttgctgg
      181 gagatgtgat caagagttcg tgaacgcggt ggtttcctca tcgaaaaccg cgcggtatca
      241 cgcgccgatg ttgcgttgga acaaacctta tgaaaccgga gtggaggtag tttatggcac
      301 tgcgccgccg tgactggggc tggctggccc tcatggttgc agtcgttatc tggggtggcc
      361 agatattcaa ccacgtcgag gcacttgccc aggatgcgta cgggagaatt ggccagatgg
      421 ccgatgaccg tgcagaggtg gaaaccgcaa ttctccggtt ttacgtcgag gacgtagacg
      481 cagggaagtt gcagctctcc caggttgacg ggatgctcaa cgaactcgac acgtacagcg
      541 attttctcaa cgagcgcgag caagaagacc tgcgcatgac tacggaaggg gccttcggcg
      601 ggcttggcat agagatcaac gcggtcgatc attaccccac ggtcatttcg ccccttgaag
      661 gaacgcccgc atgggacgcc ggcctgcttg ccggcgacca gattatcgaa atcgagaacg
      721 agagcacgta tgacgtgaag cttgatatta tcgtcggcaa gcttcggggg gtgcctggaa
      781 cggcagtaaa catcaaggtg cgccgacctg gatccagcga gcccatcgac ttccgtatca
      841 cacgtgctga gattcatgtc aactcggtcc agttccatgg cctgctgtgg gatccccgtg
      901 ctgaagggtt cctgccgctt ggtgctcctg actggttgcg gaacgctcaa gggatcgggt
      961 acatccgcct gtcttcgttc tcccgcggcg cgacggacga agtccgcgtt gcgttgcagg
     1021 acctgatggc gaagcgtgcc cggggtatcg ttcttgatct gcgaatgaat ccgggaggcc
     1081 tcctggaaga ggcacgggga gtcgctgacc ttttccttcc gaaaggtcgc ctgatcgtct
     1141 ccacgaaagg ccgggcccag aggggtgaac agaggctgta ttcggagcgc gatccggcca
     1201 ttcctccgtc gatgccgctg gtggtactgg tggacgaggg ctcagccagc gcttcggaga
     1261 tcgtcgctgc ggcgattcag gacaacgatc gtggcttgat tgtcgggcgt ccgacctttg
     1321 gcaaaggatc cgtccagacg gtgtacaact ctcagttcag cgggagattc ggtctcagtt
     1381 tttcccagaa cgcccttctg aaacttacga cggcctacta ttactccccc agcgggcgct
     1441 gcattcacaa accgcgctgg cgagcgggca agcgcggcgc ggttgtttcg aaagctgcga
     1501 gcgataccgc gacggtgtac cgtacctcga aggggagaga ggttcggggt ggtggaggcg
     1561 tggcagtgga cgtgccggtg caaccggatg ttccaccggc gctgttctgg caactcaacg
     1621 cgcggcagtt gtttttcaac tacgccgttg attacgcggt gaaacacccg gaactggatc
     1681 cgcagacatt cgacgtcaac gaagcgatgt tgtcagattt ccgcaggttc gtggcggact
     1741 cagtacacgc gttcacgtac gaaccacggg gcaaaaagga gctcgatgac ttcgcgaagg
     1801 cgacctcgga ggccggatac agtcaggaag ttacgcgtcg tgtcgaggaa ttacgggaag
     1861 ctctcctggc gcagaaagac gcggagtttt cccgtgcgga gccgtacatt cggcagcggt
     1921 tgcgcagtgc catagcaggg cgggtctggg gaaggcaatc ccgcactcga gcagatttca
     1981 cgcgcgatct cgcgctcaag gaggctctga catacctgac ggattccggc cggtacgcac
     2041 aacagattgc gctgagcccg gtacgcccgc ggcctcaaca gcaggaacag gcagccgaaa
     2101 acgaaggtga ttgagacgcg tgttgcgtct ttcgacttac gggttgggtg tggaagcgct
     2161 caacccgttt gtcgtttccc ggagatacga actcgccttt ctgtttcgtt gatagaacaa
     2221 agaaagtgga ttgcagtgga actggcaaat cagagcgatt ccgaacttat cagcatgttc
     2281 cttgacgggc aggctgccgc ttttgatacg ctggttcgtc gctacgagcg gcaggttttc
     2341 gggttcattt tcggaatggt gcagaatgag gcgctggcga acgacctttt ccaggaaacg
     2401 ttcattcgca ttatcaacgc gctgccggac taccgggaac ggggaaaatt cgcgagctgg
     2461 ttgctgggca ttgcgagaaa tctcgctctg gatgccctgc ggaagcgtcg cgttgaaggc
     2521 cggattttcc ggctggagaa tcgcacaacg gatagcggaa acgcgggttc gacatatccg
     2581 gagattcccg acgacataga aagccaggcc gatatggtgg aacgggagga atggctggtt
     2641 cgcatgaggg aggcactggc tgaattgccc gcagagcaac gcgaggttgt ctacctgcgc
     2701 tacgaagcgg atctcacctt ccagcagatc gcggacctga cgggagtctc catcaacacc
     2761 gctctcggcc gaatgcggta cgctttgaac agcttgagga agcgaatgaa cgttgccacg
     2821 gagccttcaa ccaatgaatg acacaaatgg gacaccggcg tgtcttcgca cgcaacgcga
     2881 cgccctgcgg tacacgtcag gcgaactctc ggctgaggag cgcatcacgt tcgaggtgca
     2941 tctacaacac tgccgagaat gtgcacggtt ggtggatgac gaaagaacaa tccgcagagt
     3001 gtttggagcg cttccgcgat cctctccatc ggctacggtc cgggcgttga tatcggaacg
     3061 ggctgccgcg tcaggcgctg gctaccacaa actcggcagt ccggggcgga ttgcgccgct
     3121 ccgcactgcg ctggtaaccc ggatccccgt gtggacctgg ccggttgcgg ctgccgcgat
     3181 tcttttgctc ggggtctcgt tgaccttcca cagagatgtg cgaacgccgg tatttccgag
     3241 tgacaccctg tccgcgcctg tcgcgctggt acccgatgtc ccggcaggag acggtatcca
     3301 ggttgcctcc gtttctgaaa cgacaaaggc ttcaacgcgg ataagaactc cgagggacgg
     3361 ggcaagcgca tccgacaatt tgcttgatga tgatgttgtg gagcttgcgg accgcgttga
     3421 gacgctcgca atgattctgg acgaggaaac cactcaatga aatggtcgag gacgttgtct
     3481 cttatgtgtt cggtcgttgt ggccggggtg gcttttctga acggaatcag agccgaagct
     3541 cagatgcccg ggcctccgcc gggtggtttc ccggaattct cacgtggccc caggcggggc
     3601 caactgagga accccacccc ggaggaagag gcaatggcgt tggagtttat ccgaaggata
     3661 gactcgtcgc gcgtggaaca ggtgatcgcc ctgaaaactt cccgtccaca cgcctaccgc
     3721 tcgatgattc tgcgctctct gaacatggaa cggcatgccc gcgcgatggg gagaagaggc
     3781 gatcccggtg gaatccagca atcggagcag ttgttccgaa ggatgtttga actgcgcgaa
     3841 cagatggaca ggctgtcgat ccagtaccgg ctggcaacgg acgaaaacgc gaagaacgag
     3901 attcgggcgc ggttttcggc aaaggcaggc gaacttttcg atgttcagac ggagatgaac
     3961 cgcctacaga ttgaacagat agcggacgaa ctgcgccgcc tgcaacaacg tctcgaacaa
     4021 agacaggcgg gccgcgacgc acacatccgc cgccaggtgg aaatcatgct ttccgatgag
     4081 gagcttgact gaacccatcg tccggttctc cccaccggat tctgcgcgcc aaatcctccc
     4141 cgagcatcct gcggagatct tcacgagccc ttgcggggtg tgtccccttc cagaacaaac
     4201 gaccacacaa agggcactcg gtgaagattt cctgtgtgtc gtacacgtac tcccagacgc
     4261 ggccttttac ggactctttt ggaatcacct tcaccggaac gttgcagcgt acgcaacgac
     4321 tgaatggcac caacgccgtc aggtcgaagt gtgcggccac ctgtgcaagc tggtccggca
     4381 agcggtcgaa ggtcacgccg aaacgagggg ttgagccggg caggccgagc aaccccctgt
     4441 cccgggtcaa tacgagcctg ttttcgttgc gagcccgttc catgaggtcc gcgtcggaaa
     4501 tccatgactg gtatgagaca tcggcgccga gtaacctcaa ccaggtggca aggcgcccga
     4561 gcatcatatc tgcagcgcaa ggcggtagag ggggcaaatg tagtgcttca gtcatgattg
     4621 tctctcacac tgcggttgga acccgcaaca ggctcgaggt acccttttgt tgcacatgtc
     4681 gctctgcggc ctgtcgggac ggttgccaat tcgagcactt cctctgtttt tgttcacctt
     4741 gagacttgac tgatgaaaga atgggaccga cggatcgcgc acccgattac agggcaagaa
     4801 aacccggcat acctgcacgg ggtgattgtt ccgatgttta cgccctgcaa ctcgaacggg
     4861 actctggacg aacggggtat ccgttcctat gtgaattacc tggtttcaaa cggaagcgtg
     4921 aacgcgctgt ttgcgcgaag cggactcggc cgcatgtaca cgttctccgt gaatgacgtc
     4981 cggcgtcttg cggacattgt gattgacgaa gttgccgatc gactgccggt gttcgtcggt
     5041 tgctccggcg agttgtcagc tgatagtccg gagcgaccgg acgcgcacac gtatcttcgt
     5101 cagagtatcg aactgtgtca atacgtgcaa cggaaaggag caacagccgc cgttctcgta
     5161 ttgcctgtgg cccttgtccc ccatcgcgag gaattgccgg ccgaggtggt cctggaatat
     5221 ttccggcgtg tggaggcgga aacggatatc ccgctcatca tttaccagcc gccggaaacg
     5281 acccgccgtt tccgggcaac tccgtcgctg attgaggaag tctcgcgcag cggacaggtt
     5341 gtggggatga agatttcaac gagcaggatg gacgtttttc tgagttgcag taaggcagcg
     5401 cagaacgcga atttcgcgct catcgcgggt gatgaaacgg tgttcctgtt cactatcctc
     5461 gttggtggaa cgggcgtgat cgggcaagga tgcacaaccc atccagaaat actgaaagcg
     5521 gtgtatgaga gagcgatggc gtttgacgtg gctggtgctt caaaagcggc cctggacacc
     5581 tttgccgcag taaacgcggc gcctggcgag gaccctcgac ggtgtggtct tccgatacct
     5641 ccagagggaa ggggtgaccg tacagcctta ttcaagggat gggggccgtc caccttcgga
     5701 ggaaataagc gcacgctttg cctccagtct ggacgcagtg aggtccgggt accgagattc
     5761 aatggtccgt tagtgtagtt ttccgattca agggagcctg ttgtgatgaa gagatggact
     5821 gatcccgttg tgcaaccgta caccggtcgc gcgattcctg cctacctcca cggggtgatt
     5881 accccgatgt tcacgccgtg caattccgac ggatcgttgg atgaaaaggg tatccgcgcg
     5941 ttcgtcgatt acctcatcgg aaccggttgc atcaccaccc tgttcgcgcg ttcaggattg
     6001 ggaaggatgt actcgttttc gtatgaagat gtgaagtggt ttgcggacgt cgttctcgat
     6061 caggcggcag ggcgtattcc cgttatgatc gggacgacgg gtatctgggg tcgagatttc
     6121 catcaccgtc cggggaatga gattttcact cgtcagagca tagagctttc acagtacgtt
     6181 caaaaaaaag gcgcggtggc tgcggtgctt gtccttccta tggcgctgca acctgaggaa
     6241 ggacgtcctc tggcggacac gatttttgcg tattacgaag cagtcgcacg agaggtagac
     6301 ctcccgatca tcctctacca gcaaccgggt ttgctggagg agtactgtat gactcctcca
     6361 ctgttggaac gccttctgac catcaagaac atcgcgggca tgaagtactc gactggcgac
     6421 atggtgaagt tcagcgagtt gacgatggtt gcccggaata ccgatttcgc ctttatcgcc
     6481 ggggatgaac ttgcgttctt cttcgccatg atgcttggag cgagcggggt tatcggtcag
     6541 ggttgcgacg tgaacccgga gacactgcgc gcagtatacg acagaatgat ggcccgtgat
     6601 ttcgcgggcg cacttgaagc tcactatgac tgcatacggg cggggcagat ctcagagggg
     6661 tacgattgcg tcatcagcgg gttccggtac gtggcgggca aaggcgtcag ggtgcaaccg
     6721 tttactcgcg gacacggtgt ttccgattat caggtgcagc aaccggtggt tccggatgat
     6781 tggatggcca gtttctcgcg ggaaatggac gcccttcgtc gaaagtaccc cccggttaac
     6841 ccctgggcgt aggtttggtc gtgacaagga aggcgctgat ccaagcgcct tcctttgttc
     6901 ctcgccaccc tatgccttca gttcgccgat gagcgatcgc actgaagcaa tgtcctcctg
     6961 gatgcagaaa tccctgagct cagagatcac tctcggcgcg acccacgggt cgacgaagtt
     7021 cccggtgccg atctgaacgg cggaagcccc ggcgagcatg aattccacga cgtcagtacc
     7081 cgtcatgatc cccccgatgc caattaccgg tatgcgtaca gacctgctgg ttttccacac
     7141 cattgccaga gcgaccggct tcactgcggg accggacaag cctcccgtca cgttgccaag
     7201 taccggtttc ctcgttctcg cgtcaatcgc cattccccgg atcgtgttga tgagggaaac
     7261 cgcgtctcca cctccctgct ccacgccgcg cgcgatttcc gcgatatcca tgacgttggg
     7321 actgagcttt gcaatgaccg gcaaggcgca ctcttgtttc acgcgtcgaa cgagttgctc
     7381 caccagggac ggcgcagtgc caaaatcgag cccgtgggac acgttggggc aggacacgtt
     7441 gagttcgact gcagacacac ctttttcccg ggccacacgt gccgcaagtc tgacataccc
     7501 ttccgcggtt tcgcccgcga cgttgacgat caccgggcaa gcagcctggc gaagctcggg
     7561 aagcgcatcc cgcagaaacg catccacgcc gggattcggc aatccgatgg aattcagcat
     7621 cccaccaacc gtttcacaca ctcgcggagg cggattgcct gcccgcggca gctcagtgat
     7681 tgtcttggag acaattccac ccagaagcga gaggtcgtac aatcgcgcca tctcccgacc
     7741 gtaggcaaat gtccccgacg cgacgagaac aggattggcg aggtcaagag cacctactga
     7801 cgtacgaagg tccgggctca acgtcgtgcc tgtcatcggt atcgatctca cggagtcaac
     7861 ctgctgcttc tttcagatta tacggatcaa gttcggtctc tcccagcctc tcgggtgctt
     7921 ccaaaggcgt tggagcgtcg gtggctaacc tttagcttca tgttcgggcg gcgagtaacc
     7981 gatttgcggg gagtaatgtg gactcgtcaa ccgaaaacga tgagtccgga aggaaggcaa
     8041 atggacgcga aaaagaaagc acgggtattc gttcagacgg cggcggcacc tctgagcgaa
     8101 ctcgaccgct gggtgcaagt cggcgaagaa gaagcccgtg cggtgtacga aatgacgttg
     8161 caaaacgagc tgtccggggg aacggaacag gttcgcgcgt ttgagaatga gttcagggaa
     8221 ttcaccggcc tcaaacacgc gatatccgtg atcaacggca ccgctgcaat ctggagcgcg
     8281 atgtacggtt gttatgtggg tccgggagac gaggtgatct gcccggttta tacgtggctc
     8341 tgcagtatca ctcctgcgcc gatgctgggt gcgaggcccg tgttctgcga cgtgtcaccc
     8401 cacacactcc tcatggaccc cgaagatgtg aaaaagaaaa tcacatcccg cacgagagcc
     8461 attgttgcgg tgcatttgtg gggaatggtc tgcgacatgc aggctctggg tgcgataagt
     8521 caggaaaccg gaatcccgat tatcgaggat tgctcacacg cgcacggtgc tctctttcga
     8581 ggacgcgtgg tcggggcgac tgggaaagtc ggtgcatgga gcatgcaggg ttcgaaggcg
     8641 gtgagcgccg gcgaaggagg agtgctcgcg acggacgacg acgaggtgtt tgaccgagca
     8701 tgcatgatcg gacaggtgaa tcgcgcggca ggtctggatc tcgtttcggc caaatatcag
     8761 aaatatcagc cgctggggct gggtatcaaa ttccgtgcgc acccgctcgg gattgggatt
     8821 gcgagggtcc agttgcgcaa actcgggctt ctgaacgcag gcagaggcgg gtacatcgaa
     8881 gcagttgaaa gggcactgaa ggagattccg ggactcgatc cggtcacgtt aaccgacggc
     8941 acgaaacgag gcggttacta cggtttcccg acgattttcc gcgcagagca agttccgggg
     9001 gtaacgcggg accagtacgt gaatgcgcta cgcgcgcagg gtgtacccgc ttcttcaagt
     9061 ccttacgacc tcctgcatcg cctcccgtat tttgccgaag gcatggacgt gttcggattc
     9121 gggcgcggcg ttatgtgtga ggggtacaac gggtataagg aaggggattt ccccggagca
     9181 gaggaaatgt acaggaacgt ggtctttctg ccggttctga ctgaccccgt ggaaggcgca
     9241 acggaggaaa tcatacgccg catccgcaag ggggttgagt cccttggatt ctaaggacca
     9301 tgccgcaacg cctccaagga gcgaagacag ctggatcgtg ttctcgtcac gaaggacgga
     9361 gtacgcgtgt gccgagatag gttttgtacg acccgacggc accggcctcc atttcccacg
     9421 cctcgacatt gaaggccagg tgagctggca tgccggtcct gcgttcgctg atggcgaccg
     9481 cataatcctg cacagcgtcg agaactccga gacgtggaaa caccggtcac gatccaatct
     9541 gtggatattc cggatttcca gtggcgaact gacggctttg ccgctgaaac accggcccgc
     9601 cgatttcatg ccgccagcgc ttttgctgcc gggcgatgaa cgcatggtgg tcaatcccgt
     9661 catccagggc aaccagtgcg tagtcaccat gaacctggac ggcagtgacc cggttcatgt
     9721 cacgaaaccg gaggacggct tcgcgtacgg aatcgcactc tccccggact gcagcatgct
     9781 ggcgttccac attgcggggc cgcccaacta ctttgcgtgc gtcggcgaca tcgacggagc
     9841 aaaccggcga gtcgtcgcac gggactctga cgtgcttttc ttcgctccga tgtggtccag
     9901 aaacggcgac tggctcgttt gtgaagggtg tttcagcgga aaggaccccg agcattttgt
     9961 ggcggacctt taccttgtac gaccggacgg cagggaattg cacccggtaa cgtctggcca
    10021 gagccactgg tttgcggcgg catatggcga cgagaaaagc cggagtggcg gatccaatgt
    10081 ctcgcgttgg tcgccaaccg aggacgtggt ttcgtacacg cgtttggccc cgggttcccg
    10141 gacggcgtgg aagcacattg aacagccgca agaagacgac catttcaatc gagcgtacgc
    10201 gccagaggag gctcggggcg gaacccagct gtgcgccctc aatccgttcg agcgggcgtg
    10261 ccggcccctc acggaagtga gggaaggcgt gtgggacatg cgggcggcgt ggtcgtccga
    10321 cggtgcgttg atcgcgtttt gtcgcgcaca tgtcgggcaa ccgccagggc tctgggttat
    10381 gagtgcggac ggaggacagg cccgtctgct gacggtcggg aaagagggtc tcggcgcaga
    10441 ccacccggcg tggatccacg cgagataggt atcccgcttg tggaggccaa cgcgcctaaa
    10501 gctgcttgtc gaggaaagac ggatatggaa ccgcaggaat ctggacgcag cccggttaat
    10561 tggtacacag cggacccgtg gaaggcatat ctcgggtggt taagtccgcg ctgggccgag
    10621 ccccgggaag acttcgatcc ggaggaatgg tgcggcgctt tggtggaagg cggtttccgg
    10681 acggccgttg ttcatgcaaa gcatcacgac ggtacctgct ttttcccctc tcggttccga
    10741 tctgcgcaac cgccccggga tttcttcggt gaaatcgttg gagcggccag aaagctcgga
    10801 atccgcatcg tggcgtatta ct
//
LOCUS       DAOTNG010000076        10626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5846_length_10626_cov_5.693406, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000076 DAOTNG010000000
VERSION     DAOTNG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5846_length_10626_cov_5.693406"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="P5179_05270"
     CDS             complement(<1..944)
                     /locus_tag="P5179_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94662.1"
                     /translation="MSAASMEEPGSSTLEAASRTSPDDIIDPRHEKRNYWLTVINGIM
                     TGGPSTLFDPNTVVAAFIIHLSGSQALVGLVSGLVMLGHVWPQLFVANWIEPKPKKLF
                     VYHISTVLRLASLTTLGVLIWVFRDDLPGWFVYAMIGLLFAFWSAGGFSAVAWYDILS
                     KTVYSYKRPVLFAWRQTGAGIIALLAGGVITWALSPRSGLRYPLNYLFLLSLMTVLIA
                     IGILCFNLVVEPAETHAIKKRRPFREYLRLGPQIMREDHNYRRLFWGQITFSLAVMNT
                     PFLVPFLIKDIRVDDSIIGMLMVVAAIADLLINVYWGQL"
     gene            complement(1195..2580)
                     /locus_tag="P5179_05275"
     CDS             complement(1195..2580)
                     /locus_tag="P5179_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94663.1"
                     /translation="MTVEVITRMNERNGTSGVTLVVGAGLAGLTAAYRWRQRGEDVVL
                     VEKAPYVGGMARSHYRNGFTFDFGPHFIIEKTPLLDELFAPDELVAFGPEIHMVLGGR
                     WIRYPFTPSSMLKVPLGVQMDFLRTRALGPRPGVDYGPPDSFRRNMSRQFGRGLYDFF
                     FRPYLTKKFGGWDISDVVHSDWWAMATHTRGVVTRFAPPAPQTGHAMGALRKLARLTK
                     ELPYVFRKLALWYPKAGFGEIPKRIAEQFTHNGGVIRLNCQISQIRRTENRITGVVVD
                     GEEIPVRRLIWTASPNLLARMLGTSSLDMPTLHSIIYFISTDRRYRRRGTEVRPVDPE
                     FAFYRAYFPETIWEGLTPGGRSAVVAEVGTTNRDDLAKAGEKYGAIIDTCVALGLFPR
                     NAVTDIFHDVQPDCYPVYPLDYETHLQKYYSELTPIQNLLLAGRNARFQYYNSHQVIR
                     DGERTVEDVLF"
     gene            complement(2581..3249)
                     /locus_tag="P5179_05280"
     CDS             complement(2581..3249)
                     /locus_tag="P5179_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRS94664.1"
                     /translation="MRVFWAILWKDIRYELRRRELVAVMTVFALLVVVIFAFAIGIRS
                     ESPASMAASVLWVATSFAGTLGLNRSLALERDEQCLKALLMSPADRSWVFLAKATGNL
                     VFLLAAQVILTPLVIVLFGVEDTGHWSLLGVTLLLGSAGFVGVGTLFATMATSTRMRD
                     WLLPILLFPVEVPVLIAAVKATAHVFSTAPPGESTIWIAILAAFDLIFVSMSILSFGF
                     VVEE"
     gene            complement(3249..4013)
                     /locus_tag="P5179_05285"
     CDS             complement(3249..4013)
                     /locus_tag="P5179_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS94665.1"
                     /translation="MDRVQESPRAKATPRDAEPAVEIRELVKHYGATPVLRGINLTIA
                     AGTSVALFGPNGAGKSTLLNLIATLARPTSGTIRVCGYDSVKEGPRARGCAGMLSHAP
                     WVYDRMSVRDNVRFFAKLRGKPLSDGEILDALADMDLDNVADADAGTLSRGMKQRLGI
                     VRAFLGTPALLLMDEPFTGLDRKSAGLLCDRMIRFREAGGTLLTVTHAVSEGWRIATR
                     ALMLHRGRLVQDVPVTPDGLESFAAQYDDRMRGEDD"
     gene            complement(4025..5617)
                     /locus_tag="P5179_05290"
     CDS             complement(4025..5617)
                     /locus_tag="P5179_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRS94666.1"
                     /translation="MPASRHTWLRAYHTDNPFDRSLTRPLTIVASEDIEVAWQPPGSA
                     EPKAVLSCSRYDFIFPYRTIENEERVGRGRIFLPPSCETSPKPRSVPLVLSIHYEGDQ
                     GWASGFLAQGWACMTPIALGPDHGGNLAGEGMDHTLAMAQLVRRLGFVDLQRIGWTGG
                     SAGGFQCLMAMESLWPVACANADVPVVDLYYNIRYLGTMERYNRGITDPAALVIPVIH
                     AVQSIVEKTEGYLRDDVEAYWRHSVPVGAALIRSPLIIHSATGDLLCPVAQVGDDFYR
                     PPKRSDFPPGWNMEYRKYANPLALDKPLIEWFSPEHVEQVTVAIPESAPRVDRIPVPP
                     NRPAPPPPPPPYTLVKPFSRTNLITVLIQDEGNPTPLCAHSKYAVWMDPLPYFQYHFA
                     RGYVPAEHLTPEVLTRVLGRFSKAVPQNATLPPIRRLHEHFDRWEALLALETFAGTPP
                     REENISTLRRLYSELPPVARALDVQQDGIVAQFAENPVAGLVFHQAVELRKTGETSEA
                     EAKESHLKREYGSSAFAKLRTE"
     gene            complement(5770..5845)
                     /locus_tag="P5179_05295"
     tRNA            complement(5770..5845)
                     /locus_tag="P5179_05295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5810..5812),aa:Gly,seq:ccc)
     gene            6033..7577
                     /locus_tag="P5179_05300"
     CDS             6033..7577
                     /locus_tag="P5179_05300"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRS94667.1"
                     /translation="MKGRRVLWAVSFIGVGTFIGLILASGSLFTHTPFSPFEAHAQTR
                     LFAPPSSPRTPPPPSTATAMALGEAFNWAAERAIPAVVMIKSETTVKASQNVIPNLPD
                     FFPRDLFPQIPDQSFRREGLGSGVIVSSDGYIVTNNHVIQGADKLTVVIGDDGVETSA
                     RVVGTDPRTDVAVIKVDLTNLPTLELGNSDSIKIGHWVVAIGSPFGEALRHTVTTGIV
                     SALGRNIGIVDSDNRSGYTGFESFIQTDAAINPGNSGGALVDLRGRLIGINTAISSTS
                     GSNSGVGFAIPINLVRDIARQLVENGRVSRGYLGVQIQQITPEIQNSLNLPDRHGALV
                     GDVTSGGPAARAGIQRGDVIRTINGTAVKDMNDLRLRIASTPPGQQVRLGLWREGRER
                     TVTATLDELPAEFQLPSERTSSSSRTGAVSRLGLTVTNASEATGRRGGEAQTGVLVTA
                     VDPNSPAASRGLRQGDIIQEVNLQPVANVQEFERKIAAVKAGDSVLLLVKRDNSTLFI
                     GVTIPN"
     gene            7764..>10626
                     /locus_tag="P5179_05305"
     CDS             7764..>10626
                     /locus_tag="P5179_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS94668.1"
                     /translation="MEDRLTRILELLKANELPVTWKAIIGDARGAESPEYDDSAWDEG
                     WRHLATKRWATAWLRASVPASPLLSEPISGRFVIGGHLTVWVNGMKLGEGFEPTISLP
                     VPALMANGLKIAVKSEEGGLSSEMLKLILFQSPRLRQVKTLRRSLDFVMQWRAAQPGN
                     AAAINDVLARYVAAVDPDAAPDVFLAQVVEANELLRQLDPLAKQYTVHIVPHSHVDLS
                     WGWDFSETKRIARALFDQALRIMEVDPAYTFAQDQPPMYVHLEDSATEKAIARRVKEG
                     RWDIPGATFSEPESFMPGGESWVRHLLYTKRYFKQRFGKEISLHWAPDNFSGHANTLP
                     QIWKLCGINAFVFGNWYQAPHGGQFLWEGLDGTRVFAHYMTSHYDSAQMIEQDKVIRN
                     VCEHMKATTIPACMLLDGDDLTPPWPGSPQGIENLRALAAFPKIEFSTPHRFFSSVDT
                     AADGLRVVQGEFISTTDHRHNNVGAYSTFAEVKLRNRRSEWGLRTLEAMATLAMRDGG
                     IYPTKHAARAWRLTLFNQMHDIFPGTAIFEAYEEAYKRYDEVDSICSIGTNAVANVLA
                     STINTKGDGIPVVLFNTLGWDRNDPAEILLTEPHSYCDGFEVRDGNGRPVPAQTLSTD
                     IGTFDKTNKNFRLLVQPDGIPAMGYKTVWLHPAHRDERVFSSMVGADRLTLDNDFVRV
                     TINPRTGWVSQLFDKRLNRNVLPDGKEACAIEGQGDVGNPWHLCPEGPVRWMNQTVRT
                     EVVEDGDVRAAIRVTTTWHRSTFVQEFRITRTSPRVEVHTTIDCHEANFTMKALIPVA
                     LPADAPWTCEVPWGAVQRPIPDNDRATQTWMDVSNGQWGLSVLNNGRYGHSRRADGTM
                     TVTLLRTTVAHKSQDQSDEGRHEFTYALLPHKGNWTEAATVREAHMLNAPIITTREIP
                     HEGARPDTHGMIRIGAPNIVLAALKKAEDNDDWVLHLYET"
BASE COUNT         2240 a         3107 c         3335 g         1944 t
ORIGIN      
        1 agctgacccc agtacacgtt gattaggagg tccgcgattg cggccaccac catgagcatg
       61 ccgatgatgc tgtcatccac gcggatatct ttgatgagga acggcaccag aaacggggtg
      121 ttcatgacgg cgagggaaaa ggtgatctgc ccccagaaca ggcgtcggta attgtggtcc
      181 tcgcgcatga tctgcggccc cagtctcaga tactctctga atggccggcg ttttttgatg
      241 gcatgcgttt cggcgggttc caccaccagg ttgaagcaga ggatcccgat ggcgatgagg
      301 accgtcatga gcgagaggag gaagaggtaa ttcagcgggt agcgcagtcc tgagcggggg
      361 ctgagcgccc atgtaatcac gccgccggcg agcagggcga tgataccggc gccggtctgc
      421 ctccaggcaa aaagaacagg ccgtttgtag ctgtacaccg tcttgctcag gatatcatac
      481 caggcaacgg cactgaaccc acccgcagac cagaaggcaa acaacaggcc gatcatggcg
      541 tagacgaacc accccggcag gtcatcacgg aagacccaga tgagcacgcc aagggtagtg
      601 aggctggcaa ggcgcaacac cgttgagatg tggtacacaa acagcttttt cggtttcggt
      661 tcgatccagt tggcaacgaa aagctgcggc cagacatggc ccagcatgac cagtccgctt
      721 accagaccga cgagagcctg agagccgctg aggtggatga tgaacgccgc gacaaccgtg
      781 ttcggatcga acagggtgct tggccctccc gtcataatgc cgttgatgac ggtgagccag
      841 tagttgcgct tctcgtgtct ggggtcgatg atgtcgtcag gggaagttcg gctggcggct
      901 tcaagggtgg aagagccggg ctcttccatg gatgctgcac tcaaggacca atgtgctccg
      961 tatatcaacc ccgcgattcc tcgcggggaa catgatcggc agacgcggcc gcgcctggtt
     1021 ccgggcctcg cgcgcggaac tgcggacaca cgccgggata tcggatttca gcttgtggaa
     1081 ttaacgctct cttaatatac cgaaacctcg ggttttgacc ccaggggaat acacgaaaag
     1141 tgatccatgt gcagtgagat cgcgtacgcg ggcgacgcat cgaagcagtg gagatcagaa
     1201 cagcacgtcc tcgaccgtgc gttccccgtc gcgaatgacc tgatgagaat tgtagtactg
     1261 gaatcgcgcg ttgcggccgg caagcaggag attctggatc ggtgtaagct cagaatagta
     1321 cttctgaagg tgagtttcgt aatcgagggg atacaccggg tagcaatcgg gttgcacatc
     1381 gtggaaaatg tcggttaccg cgttgcgggg aaacagcccc agagccacgc aggtgtcgat
     1441 tatggctcca tacttttcgc ccgcttttgc gagatcgtca cgattcgtgg ttccgacttc
     1501 ggcaaccacc gcgcttctgc cgccgggtgt aagaccttcc cagatggttt ccggaaaata
     1561 ggcgcggtaa aacgcgaatt cagggtccac cggccttact tcagttccac gccggcggta
     1621 ccggcgatcg gtgctgatga aatagatgat cgaatgcaat gtcggcatgt cgagagatga
     1681 cgttccgagc atgcgcgcaa gcaggttggg agatgccgtc cagataaggc gccgcaccgg
     1741 gatttcctct ccgtccacta ccacgccggt gatacggttc tcggtccggc gaatctggga
     1801 aatctggcaa ttcaggcgaa tcactccgcc gttgtgagtg aactgctcgg cgatgcgctt
     1861 cggtatctcc ccaaacccgg ctttggggta ccacagggcc agtttcctga acacgtacgg
     1921 gagttctttc gtcagccgcg caagcttccg gagggcgccc atcgcgtgcc cggtctgcgg
     1981 ggcaggcggt gcgaagcgcg tgaccacgcc ccgggtatgg gttgccatcg cccaccagtc
     2041 actgtgaaca acatcgctga tatcccaccc gccgaacttc ttcgtcaggt acgggcggaa
     2101 gaagaagtcg tacagcccgc gcccgaactg gcggctcatg tttcgccgga aggagtccgg
     2161 cggcccgtaa tccaccccgg gtcgggggcc caaagcgcgt gtccgcagga agtccatctg
     2221 cacgcccagc ggcactttca gcatggagga gggtgtgaac ggataccgaa tccaccggcc
     2281 tccgagcacc atgtggattt cggggccaaa tgcgaccagt tcgtccgggg cgaaaagctc
     2341 atccagcagg ggcgttttct cgatgatgaa atgcggaccg aaatcaaagg tgaacccgtt
     2401 ccggtagtgc gaccgcgcca tgccgcccac gtacggagct ttttcgacca ggacgacgtc
     2461 ctcaccgcgc tgacgccagc ggtacgctgc ggtcagcccc gcaaggcccg ctccgacgac
     2521 caacgtaacg cctgatgtgc cattccgctc attcatgcgc gtaatcacct cgacagtcac
     2581 tcattcctcg accacaaacc cgaacgagag aatggacatt gagacgaaaa tgagatcgaa
     2641 ggccgcaagt atggcgatcc agatggtgga ctccccgggc ggcgcggtgg aaaacacatg
     2701 tgcggtcgcc ttcaccgcgg cgatgagcac cggaacctcg acgggaaaca ggaggatcgg
     2761 gagaagccaa tcccgcatgc gcgtgctcgt ggccatcgtg gcgaacaggg tgcctactcc
     2821 gacaaaccct gctgacccca gcagaagcgt gacgccgagc agcgaccagt gacccgtatc
     2881 ttcaaccccg aacagcacga taacgagggg agtaagaatc acctgggctg ccagaaggaa
     2941 taccaggttg ccggttgctt tggcaagaaa cacccagctt ctgtccgccg ggctcatcaa
     3001 cagggctttg aggcactgct cgtcgcgctc aagggcaagg gaacggttca ggccgagcgt
     3061 tccggcgaat gaggttgcca cccagaggac ggaggcagcc atggaggccg gcgattcgga
     3121 gcgtatgccg atcgcaaacg cgaatatcac gacgacaagc aacgcgaaca ccgtcatgac
     3181 ggcgacgagt tcccgccttc gaagttcgta gcgaatatct ttccacagaa tcgcccagaa
     3241 cacccgcatc agtcgtcttc tccccgcatg cggtcgtcgt actgagccgc aaacgactcc
     3301 aatccatccg gcgtgaccgg gacatcctgc accagccttc cccggtggag catcagagcg
     3361 cgggtggcga tccgccagcc ttcactgacc gcgtgcgtga cagtgaggag tgttccccct
     3421 gcctcgcgga agcggatcat gcgatcgcag aggaggccgg cggacttccg gtcgaggccg
     3481 gtgaacggtt catccataag gagaagggca ggggtgccga gaaaggcgcg aacgattccg
     3541 agccgttgtt tcatgccgcg ggagagggtt cccgcatcgg cgtcggcaac gttatccaag
     3601 tccatatccg ccagcgcgtc cagaatttct ccatcgctga gaggctttcc cctcagtttc
     3661 gcgaaaaagc gcacgttatc ccgcacggac atccggtcat acacccacgg cgcatgggag
     3721 agcatgcctg cacatccgcg agcgcgcgga ccctccttca ccgaatcgta tccgcacact
     3781 cgaatcgtgc cagacgtagg gcgggcgagg gtggctatga gattgaggag ggttgatttc
     3841 cctgcgccgt tcgggccgaa cagggcgacg gacgtaccgg cggcgatggt gagattgatt
     3901 cctcgaagaa ccggggtcgc gccgtagtgc tttacgagct cccggatctc caccgccggt
     3961 tccgcatctc gcggcgtggc tttcgcccgg ggagactcct gcacccggtc caccggagaa
     4021 acccttactc cgtccggagt tttgcgaagg cggaagaacc gtactccctt ttgaggtggg
     4081 attctttcgc ttctgcttcg gaggtttcgc cagtttttcg caactcaacc gcctggtgga
     4141 aaacgaggcc tgcgaccggg ttttcggcaa actgagcgac aatgccgtcc tgttgcacgt
     4201 ccagagccct cgcaaccggc ggaagctccg aatacagcct gcggagagtg ctgatatttt
     4261 cctcgcgagg cggggttccg gcgaacgttt ccagcgcgag cagcgcctcc cagcggtcga
     4321 aatgctcgtg caaacggcga atgggcggaa gtgtcgcatt ttggggcacg gcctttgaga
     4381 aacgacccag aacccttgtc agcacttcgg gagtgaggtg ctccgcagga acgtatcccc
     4441 gcgcaaagtg atactggaaa tagggcagcg ggtccatcca gacggcgtac ttcgaatgcg
     4501 cgcacagggg agtcgggttg ccttcatcct ggatcaacac ggtaatgagg ttggttcggc
     4561 tgaatggctt tacgagtgtg tacggcggtg gcgggggcgg aggcgccggc cggttgggtg
     4621 ggacggggat gcggtccaca cgtggggcgg attccgggat tgccacggta acctgttcca
     4681 cgtgttccgg cgaaaaccat tcgatcagcg gcttgtccag cgcgagtgga ttcgcatact
     4741 tgcgatattc catgttccag cccggcggga agtcgctccg cttcggcggg cggtagaagt
     4801 cgtcgccgac ctgcgcaacc ggacaaagga ggtcaccggt cgcactgtgg atgatcagcg
     4861 gggacctgat gagtgccgca ccgaccggta cggaatgccg ccagtacgcc tccacgtcgt
     4921 ccctcagata tccttccgtt ttttcgacga tggactgaac cgcgtgaatg accggtatca
     4981 caagcgcggc ggggtccgtt atgccgcggt tgtaccgctc catcgtgccg agatacctga
     5041 tgttgtaata gaggtcaaca actggaacgt cggcgtttgc gcacgccacc ggccacagcg
     5101 attccatcgc catgaggcac tggaagccgc ccgcagaccc ccccgtccac ccgattcgct
     5161 ggaggtccac aaacccgaga cggcgcacga gctgcgccat ggcgagcgtg tggtccattc
     5221 cttcgccggc gaggttgcct ccgtggtctg gtcccagggc tatgggagtc atacaggccc
     5281 atccctgggc gaggaaaccc gacgcccatc cctgatcgcc ctcgtaatga atgctcagca
     5341 caagcggcac gctgcgaggc ttcggagaag tttcgcagga tggcgggaga aaaatgcggc
     5401 cacgccccac gcgttcttcg ttttcgatgg tccggtaggg gaagatgaaa tcatagcggc
     5461 tgcacgaaag aaccgccttc ggttccgcgc tgccgggcgg ctgccacgct acttcgatat
     5521 cctcactcgc gacgatggtg agggggcgcg tcagcgatcg gtcgaagggg ttatccgtgt
     5581 gatacgcgcg taaccaggtg tggcgtgaag ccggcatgaa tacctccacg gagtgtgccg
     5641 aaagaagagg tgctgcctat ggaatgtagc gcagcggcgg ggtcgggaca attcacaaaa
     5701 aaaccgccgg ctccttcgag gcggcggcgt atgcaaacgc atcgctgatc cgatgcggaa
     5761 tggaacgaat ggagcgagcg acgggattcg aacccgcgac agccagcttg ggaagctgga
     5821 actctaccag ctgagttacg ctcgcgtact acctagtata gcggttgcgc gaagaatcgt
     5881 caagcgctga acgcacgtcg gcggggtgtt caaggaaccg cgcccccggg gcgcgcgttc
     5941 cacattttga atcgcgtgtt ccggtcgtac acttagtagt gagtgaacca tctcacgttc
     6001 cctggttttt cgcaaaaagg aaggatacca ccatgaaggg gcgaagagtc ctctgggctg
     6061 tttcgttcat cggtgtaggc acgttcatcg gattgatcct tgcttcgggg tcacttttta
     6121 ctcacacgcc gttttctcca tttgaagcgc acgctcagac gcggttattc gcgccgccat
     6181 caagccctcg cacgccgcca ccaccttcca cggcaacggc gatggcactg ggtgaggcgt
     6241 tcaactgggc cgccgaacgg gcgattccgg cggtcgtaat gatcaagagc gaaacgaccg
     6301 tcaaagcttc ccagaacgtc attccgaacc tgccggactt tttcccgcgc gatcttttcc
     6361 cgcagatacc ggaccagagt tttcgccgtg aagggctcgg cagcggcgtg atcgtcagtt
     6421 cggacgggta catcgtcacc aacaaccacg tgattcaggg cgcggacaag ctgacagtgg
     6481 tcatcggaga cgacggagta gaaacgagcg cccgggttgt tggaaccgac ccacggacgg
     6541 atgttgcggt gatcaaggtt gatttgacga acctcccgac gctggagctg ggaaacagcg
     6601 acagcatcaa aatcgggcac tgggtggtgg cgatcgggag tccgttcggg gaagccctgc
     6661 gacatacggt gaccaccgga atcgtcagcg cgctggggcg aaatatcggg atcgtggaca
     6721 gtgacaatcg cagcgggtat accggtttcg aaagcttcat ccagaccgat gcggcaatca
     6781 atccgggcaa ctccggcggc gcgctggtgg atctgcgcgg gcggttgatc gggatcaata
     6841 cggcaatcag ttccaccagc ggcagcaatt ccggcgtggg gtttgccata ccgatcaatc
     6901 tggttcggga tattgcccgg cagctggtgg aaaacggccg tgtttcgcgt ggatatctgg
     6961 gcgtccagat tcagcagatt acgccagaga ttcagaacag cctcaatctg ccggaccggc
     7021 acggcgcact ggtgggtgat gttacttccg gaggccccgc tgcgcgcgcc ggaattcagc
     7081 gcggcgacgt aatcagaacg atcaacggga cagcagtgaa agacatgaac gatctccgtc
     7141 tccgcatcgc cagcacgcct cccggccaac aggtccgact cgggttgtgg cgggaaggca
     7201 gagagcgcac tgtaaccgcg acgcttgatg aattgcccgc ggagttccag ctccccagcg
     7261 agcggaccag ctcgtcatca cgcaccggag cggtgtccag actggggctc accgtgacaa
     7321 acgcatcgga agcgacaggg cggcgcggcg gagaagcgca gactggtgtt ctcgtgacgg
     7381 cggtggaccc gaacagcccc gcggccagcc gtggcctgcg ccagggggac atcattcagg
     7441 aggtcaatct gcagcccgtg gcgaacgtgc aggaattcga gcgtaagatc gccgcggtga
     7501 aagcggggga ctcggtgctg ctcctcgtca aacgggacaa cagcacgctg ttcatcggcg
     7561 tgacaatacc gaactgaaaa cccgtagaga acgatgcttt cggacaggcg ggagcgggcg
     7621 cgcaaacgcg cccgctcctt ttttgttccg gtggttcgcc gtcgtctcgc ccgctgagcg
     7681 ttgttccgca gagatcgcgc atttatgtta cgtctgcaac gatttggcca cccgcacaga
     7741 ccccgaattt ccggagaacc cacatggaag accgccttac ccgaattctg gaactgctca
     7801 aggcaaatga actgccggtg acgtggaaag cgatcatcgg agacgcacgc ggcgcggaat
     7861 cgccggaata cgatgattcg gcgtgggacg aggggtggcg gcatctggca acgaaacgct
     7921 gggccaccgc gtggttgcgg gcttccgttc ccgcgtctcc cctgctttcc gagccaatca
     7981 gcggaagatt cgtaatcgga ggccacctga cggtctgggt gaacggaatg aaacttggcg
     8041 aggggttcga gccaacgatc agcctcccgg tgcctgctct catggcgaac gggctcaaga
     8101 ttgccgtgaa atcggaggag ggcgggcttt cgagcgagat gctgaagctg atcctgttcc
     8161 aatcaccccg gttgcggcag gtgaaaacac ttcgacgcag ccttgatttc gtgatgcaat
     8221 ggcgcgcggc gcaacccggg aacgcggcgg cgatcaacga tgttctggcg cggtacgttg
     8281 cggcggtgga ccccgatgct gcgccagacg tgtttttggc gcaagtcgtg gaggcgaacg
     8341 agctgctcag gcaactggat ccgctggcga agcagtacac agtgcacatc gtcccgcact
     8401 cgcacgtgga tctctcctgg ggatgggatt tctctgagac aaagcgaatc gcgcgcgcgc
     8461 tgtttgatca ggcgctgcga atcatggagg tcgaccctgc ctacacgttc gcgcaggatc
     8521 agccaccgat gtacgtgcat ctggaggatt cggccaccga aaaggcgatt gcccgtcgcg
     8581 tgaaagaagg tcgatgggat attccgggag caacgttcag cgaaccggaa agcttcatgc
     8641 cggggggcga aagctgggtg cggcacctgc tgtacacgaa acggtacttc aaacagcgct
     8701 ttggcaagga aatttcgctg cactgggcac ccgacaactt ctccggccac gccaacactc
     8761 tgccgcagat ctggaagctg tgcggaatca acgcgttcgt gttcgggaac tggtatcagg
     8821 cgccgcacgg ggggcagttt ttgtgggagg gcctggacgg gacccgcgtg ttcgcgcact
     8881 acatgaccag ccactatgat tccgcgcaga tgatcgaaca ggacaaagtc atccgcaacg
     8941 tgtgcgagca catgaaggcg acgaccattc cggcgtgcat gctgctggac ggggacgacc
     9001 ttactccgcc gtggccggga agcccgcagg gtattgagaa tctgcgcgcg ctcgcggcat
     9061 ttccgaaaat cgagttttcc acgccgcacc ggttcttttc ttccgttgat accgccgcgg
     9121 acggcttgcg cgtggtgcag ggcgagttta tcagcacaac ggaccaccga cacaacaacg
     9181 tgggagcgta cagcaccttc gcagaggtga agttgcgcaa ccgtcgttcg gaatgggggc
     9241 tgcgtacgct ggaggcgatg gcgacgcttg caatgcgcga cggcgggatt tatccgacca
     9301 aacatgccgc gcgggcgtgg cgcctgacgc ttttcaacca gatgcacgac atcttcccgg
     9361 ggacggccat ctttgaagcc tacgaggagg cgtacaagcg ctacgacgag gtggacagca
     9421 tctgctccat cggaacgaac gccgtggcaa acgtgctcgc ctctacgatc aataccaaag
     9481 gggatggtat ccccgtcgtg ctcttcaaca cgctcgggtg ggaccgaaat gacccggcgg
     9541 agatcctgct caccgagccg cacagctact gcgacgggtt cgaagtccgc gacgggaacg
     9601 ggaggccggt tccggcgcag accctttcca ccgatatcgg cacattcgac aagacgaaca
     9661 agaacttccg ccttctcgtc cagccggatg ggattccggc catggggtac aaaacggtct
     9721 ggctccaccc cgcgcaccgt gatgaacgag tgttctcatc aatggtgggc gcggaccgac
     9781 tgaccctgga caacgacttc gttcgcgtaa cgatcaaccc gcgaacgggc tgggtatcac
     9841 agttgttcga taagcggctg aaccggaacg tgctcccgga cggaaaggaa gcctgtgcga
     9901 ttgaaggaca gggcgacgtc ggaaatccgt ggcatctgtg cccggaaggc ccggttcgct
     9961 ggatgaacca gaccgtacgg accgaagtgg tcgaagatgg cgacgtgcgt gcggcaatcc
    10021 gcgtgacaac gacctggcat cgttcgacat tcgtgcagga gttccgcatc acccgtacca
    10081 gtccgcgcgt ggaagttcat accaccatcg attgtcacga ggccaatttc acgatgaagg
    10141 cgctcattcc ggttgcgctg ccggccgatg ctccgtggac gtgcgaagtg ccgtggggcg
    10201 ccgtccagcg gccgattccg gacaacgacc gcgcaacgca gacgtggatg gacgtctcga
    10261 atggtcagtg ggggctttcg gtgctcaaca acggacggta cggccattcc cgccgcgctg
    10321 acggcacgat gactgtcacc cttcttcgca caaccgtggc gcataaatcg caggatcagt
    10381 ccgacgaagg gcgacacgag ttcacctacg cgcttctgcc tcacaaggga aactggactg
    10441 aagccgccac ggtgcgcgaa gcccacatgc ttaacgcgcc catcatcacg acacgcgaga
    10501 ttccacacga aggtgcgcga cccgacaccc acggcatgat acgaattgga gcgccgaaca
    10561 tcgttcttgc ggcgctgaag aaggcggagg acaacgacga ctgggtgctt cacctgtacg
    10621 aaacca
//
LOCUS       DAOTNG010000077        10580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5889_length_10580_cov_5.234679, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000077 DAOTNG010000000
VERSION     DAOTNG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_10580_cov_5.234679"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1540
                     /locus_tag="P5179_05310"
     CDS             110..1540
                     /locus_tag="P5179_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS94669.1"
                     /translation="MEATERVKGANGTVLIVDDDPAIRKLLAIALERAGFQVRQAEGY
                     RQCYGEMSDTASVDAVLLDIMLPEVNGLEILRHCSGLDSPPAIVMMTASTDVSDAVSA
                     LKLGAFDYLIKPDDTSNLPKLTGVLRNAVSFTRSKREIVRLKRELVARGRPAEIVGTS
                     APMQRVFEAIRQVADSDASVLITGESGTGKELVAKAIHEASARSRGPFVVVNCSAIPI
                     TLIESELFGHEKGAFTGAIAQKRGKFEIAHGGTIFLDEIGDMDSGSQTRLLRVLQEQA
                     FERVGGTHTINVDVRVIAATNQDLQARVRESAFRLDLFYRIAVFAISLPPLRDRTEDI
                     PLLAAHFLNLHASKAHKEATGFEPAAMNALVRHAWPGNVRELGNVIQHAVIVSEGPTI
                     RLEHLPPHLVQASVPETPAGENAVLLRDPSTHDIRPLKEVEKEVMKRALEATEGNFAL
                     AAMKLQIGRATLYRKVKEYGLDADGT"
     gene            1609..2127
                     /locus_tag="P5179_05315"
     CDS             1609..2127
                     /locus_tag="P5179_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94670.1"
                     /translation="MSPYVNRIRRALKLDPALFEEVEADRHATGEAVGVVLLSSGATG
                     LGAIPQTSIGSLAILFLAVLVGWCAWVYVTFVFGVQVFSVPETDVDVGQIVRTSGYAA
                     APGLFAIFGIIPLLYVPALALALVWMLAAMVVSVRQAFDYSSTWRALGVCAAGWIVLA
                     AYIGGVVLLLRC"
     gene            2163..3443
                     /locus_tag="P5179_05320"
     CDS             2163..3443
                     /locus_tag="P5179_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS94671.1"
                     /translation="MEASRVRVLASLSALAFSAVAFAVAGLFVDRQLQALLDDRLAEA
                     LLAQAKTVAGAVFLENTTDSVALALATGTLSATREQTGAELVVLLDARGRVLFTDPPV
                     LGEHPYIDLDNAALASAITGKPACGERFRIGNVDLKTAFAPVFNPFGEVVAIVGIEAP
                     ADFFGALRDVRRALVVTLGLGMALVAGLVAVVWHFWARSEASERALWDSQQLAMIGQM
                     TATMAHEVRNPLGIIKAAAERIRRKYGDGTELFDFIPDEVDRLDRLTEWYLRFARPGD
                     LRLAKARLGPIVRDSISRVRSELEAAAIEVVVEDLAQSECLVDYDRLVQVNINILLNA
                     RQAIGSNGRVEAKVIEKSGVVRVEVKDNGPGIPACIQPNVFQPFFTTKTAGSGLGLPV
                     MKQVVEALGGRVGLNSRPGEGSTVWYELPCAKNT"
     gene            3466..3804
                     /locus_tag="P5179_05325"
     CDS             3466..3804
                     /locus_tag="P5179_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94672.1"
                     /translation="MQRDKVGKIVPIGKGLCRVIVRSRKLAERLSSVPGVEVRAQDAE
                     YLGWWIIFPESMRALIEPVFREKTIVTEHASQLSLLDMDADDGGNSQEESPQKCGDGA
                     GEGNGSGTPG"
     gene            3782..5101
                     /locus_tag="P5179_05330"
     CDS             3782..5101
                     /locus_tag="P5179_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS94673.1"
                     /translation="MARVLLVDDERKLAVLLAGELTDAGHWVLTAQDGRTAIQLVKEQ
                     AFDVVVTDIRMEEVDGLALLRAVKDTCPDTDVIMMTAYASIETTVEALRKGAADYLIK
                     PFPPEELILAVERVVERQRLALENRQLKRELASAESEFIAASDSMKAVADVVSRIAKT
                     DTSVLITGASGTGKEVVARAIHARSKRKDGPFVAVNCAALPEPLLESELFGHERGAFT
                     GADRRKLGRFELADKGTLFLDEIGEIGQSIQVKLLRALETKTFERVGGTTNVSVDVRI
                     IAATNKDLPTAIANKQFREDLYYRLNVFPITLPPLKERVADIRALAAHFVAPTGLKIS
                     DAALRQLEEYEWPGNVRELRNVLERAVILCDGDTILPEHLSLNPISRQPGNDGGAADL
                     NLERRERKALEEALARAKGNKAEAARLLGISRRALYSRAASLGITLT"
     gene            complement(5165..5338)
                     /locus_tag="P5179_05335"
     CDS             complement(5165..5338)
                     /locus_tag="P5179_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS94674.1"
                     /translation="MPAVVNKEECTGCGSCVEVCPSEAITMDDDVAVVNPEECTECGA
                     CVDECPSEAITLE"
     gene            complement(5394..6467)
                     /locus_tag="P5179_05340"
     CDS             complement(5394..6467)
                     /locus_tag="P5179_05340"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRS94675.1"
                     /translation="MLTKGLLLCAGMGSRLRPITYTSAKHVLPVANKPLIHYTIEMLH
                     RAGITEIGIVVGVNRHEIEGAVGDGSRWDVKVDYIVQDNPLGTAHAVSLARDWAAGAP
                     FLMVLGDILVENGINSVVEAFAKNQPASVVVLHKTENPRQFGIAEIENGRIVGLEEKP
                     ESPKSNLAVAGIYVFQPCIFDVIGNVKPSLRGELELPDALRELIARNLVVQPLMLEGW
                     WKDTGRPEDMIDLNRLLLEKILEASCHGNVDGESSVVGRVVIESSAIVERSIIRGPVI
                     VGPGSTIRDAYIGPYTSIGPGVTIEGSEVEYSIIMEKSVISGIKSRIGSSLIGKNVRI
                     THERLMPSENRFVLGDSSLVNFA"
     assembly_gap    6480..6489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6494..7765)
                     /locus_tag="P5179_05345"
     CDS             complement(6494..7765)
                     /locus_tag="P5179_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRS94676.1"
                     /translation="MAIINPFRGIRYNSEKVGALQTVVAPPYDVISPEGRAQLLSKNL
                     FNVVRIDLPIGDGDAKYRYAGSLWRRWRESGVLVRDPAPALYFLEERYVTPSGRPSAR
                     RGFIGELVLEEIGKGSVLPHERTFAGPKADRLRLMEETLANLSPVFLLYRDPMDELGE
                     TYSRTASYPPDVEVETPDGTSRMWRVTDGDDIRRVAEVVRRYPLIIADGHHRYETAIR
                     FRDQQRSMGIRGAAESLMVYLCSSDDPDLTILPPHRVVTFPPSFDMDGFVSRLETYFT
                     VTAVDATSPNLRGLLGKSAGEPYEIGMWVKNRGGFVLRLRSWERVAEWIDPQRSLAWR
                     KLDVSLLHEVVLKRIAGVSENDMNIRYTVDPEDGCSAVVSGSADIFFLIRAPSAAQIA
                     EVAEAGDVMPHKSTYFSPKPLTGMVMRSLSD"
     gene            complement(7765..8901)
                     /gene="ispG"
                     /locus_tag="P5179_05350"
                     /gene_synonym="gcpE"
     CDS             complement(7765..8901)
                     /gene="ispG"
                     /locus_tag="P5179_05350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRS94677.1"
                     /translation="MAHENPGSAGLGNDTSSHVKPLTLRTATRRVSVGGVQIGGGAPV
                     TVQSMCTTNTRDIDATVRQIHALADAGCEIVRVAVPHEADARALGEIRRQIPIPLVAD
                     IHFGHRLALVAVEQGVDKLRINPGNIGTRQSVERVASACSERGIPIRIGVNAGSLEKD
                     ILEKFGYPTAAGLVESALRHVRILEDLSFYDIVVSLKASDVPMAIAAYTDFSRERPYP
                     LHVGITEAGTAKTGTIASAVGIGAILSAGIGDTIRVSLTDDPVKEVAVGFDILKSLRL
                     RVKGPTLISCPTCGRVEIDLIRIAQEVERRLEKYSESFRVAVMGCVVNGPGESQGADF
                     GIAGGKGVGLVYRDGAVVRRVKEEELVAALFEEIEKARAEGRVS"
     gene            complement(9042..>10580)
                     /locus_tag="P5179_05355"
     CDS             complement(9042..>10580)
                     /locus_tag="P5179_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94678.1"
                     /translation="TSGDVFRGHAEVRAPVFRVTSSAKWEGEQDTRDSPRERLYGTRF
                     QDWSAGLAISETEHFAISTEVQSRYNSAYDSLFASWVRQQRSYTQTWRGAVRSWKGLS
                     STVEYGWRKRQNLAGGGSTVSDNADVDIRHDALGGGVRNAFRYRIASTRTAKRERLFL
                     YVGAGRGDYAPIDPNDTRAILAETEVVIAPAGDPTASYVLRYRDTDDFSPTVTLDAAW
                     QLNTDFNRFWKGAANPRTNEIRPLWQRAIRLVGTETNVLISETDTSRTSDLYLLKLWT
                     FRKPGKSPTLRGSVRVTQDLLLRDPSWAGDLRFRIMTYEDYDAALYSRAGMPAVTNQK
                     DYSVRGRLRVRPGTDWTAELKWQSDLRNGSNQDYSVRRNWLDQSITYRNSSFDEFSLR
                     NEAGEGKDRVPAAAYVAVQGKPLEAYVISLEPAWRKSWGNRGVMRTSAQWSGVFVRNG
                     THGGMLPVHLLNGRNGGHNFRASMLASYRLSSLATLSVSYSARRNAGTRLTHTARAEF
                     NAVF"
BASE COUNT         2217 a         3045 c         3108 g         2200 t
ORIGIN      
        1 aaactcgtac actgaatctg cggggtgccg gtgaccggaa tccgaaaagc cgcattaact
       61 ggcagaataa aaggggggca gggaaacgga cacgctctgg caggcggaca tggaagcaac
      121 cgaacgcgtc aaaggcgcaa acgggaccgt actcatagtc gatgatgatc cggcgattcg
      181 caagttgctg gcaatcgcgc tggaaagagc gggtttccag gtgcggcaag cggagggata
      241 ccgccagtgc tacggcgaga tgagtgacac ggcgagcgtt gacgcagtcc tcctcgatat
      301 catgttgccc gaagtaaacg ggctggaaat cctgcgccat tgttccggac tggattcccc
      361 gcccgctatc gtaatgatga ccgcctcaac ggatgtctca gacgccgtca gcgccctgaa
      421 actcggtgcg ttcgattacc tgatcaagcc ggatgacacg agcaatctgc ccaagcttac
      481 cggagtactg aggaacgcag tctccttcac acgttcaaaa cgcgagatcg ttcggttaaa
      541 gcgggaactg gtagcgcgcg gccgcccggc ggaaattgtt ggcacctcgg cacccatgca
      601 aagggtcttc gaggcgatcc gccaggtcgc tgacagcgat gcctctgttc tgatcacagg
      661 ggaaagcggt accgggaagg agttggtggc aaaggcgatt cacgaggcaa gcgctcgttc
      721 caggggacct tttgtcgtcg tgaattgctc ggcgattcca atcacgctca ttgaaagcga
      781 gctgttcggg catgaaaaag gagcattcac aggcgcaata gctcaaaaga ggggcaaatt
      841 cgagatcgcg cacggcggga ccattttcct cgacgagata ggagacatgg attccggttc
      901 gcagacaagg ttgctgcgtg tgctccagga acaggcgttc gagcgcgtag gaggcacgca
      961 caccatcaat gttgacgttc gggtcattgc cgcaaccaat caggacctgc aggcccgtgt
     1021 tcgggaatcg gcatttcggt tggacctgtt ctaccggatc gcggtctttg ccatctcatt
     1081 gccgccgcta cgcgatcgaa cggaggatat tccgctcctt gccgcacatt tcctgaacct
     1141 tcacgcgtcg aaagcccaca aagaagctac cgggttcgaa ccggcggcga tgaacgctct
     1201 tgtccggcac gcatggccgg ggaatgttcg ggaactggga aatgtgatcc aacacgcggt
     1261 aatcgtttca gaaggaccaa ccatccggct tgaacatctc ccgccacacc tggtgcaggc
     1321 gagtgttccc gaaacgcctg cgggagagaa cgccgtgctt cttcgggatc cttccacaca
     1381 cgatattcgt ccgctgaaag aagtggagaa ggaggtgatg aaacgggcgc ttgaggccac
     1441 ggagggcaac tttgctctgg ctgccatgaa gcttcaaatc ggccgggcca cgttgtaccg
     1501 caaggtgaag gaatacgggt tggacgcaga cggaacttga cggttctgag cgtgcatgtt
     1561 ccccatctcg actccgtggg tgtgcaaaag gcgatgatgg agggcagtat gtctccttat
     1621 gtgaatcgaa tacggcgcgc actgaagctc gatcccgcat tgttcgagga agtggaagcg
     1681 gacaggcacg caacgggcga agccgttggt gtagtcctgt tatcgagcgg cgcgacgggc
     1741 cttggagcga ttcctcagac aagcatcgga tcgctcgcga ttctgtttct cgcggtgctc
     1801 gtcgggtggt gtgcatgggt atatgtgaca ttcgtgttcg gggttcaggt gttttctgtg
     1861 cccgaaacgg atgttgacgt gggccagata gtacgcacgt cggggtacgc cgctgcgccc
     1921 ggtctcttcg ccattttcgg catcattccc cttctctatg ttcccgcgct tgcgctggcg
     1981 ctggtgtgga tgctcgccgc aatggttgtg tcggttcgcc aggcgttcga ctactcaagc
     2041 acctggcgtg ctctcggcgt gtgcgcagcc ggttggatag ttcttgcggc gtacatcggc
     2101 ggggttgttc tcctgttgcg gtgctgataa gcgcgggtac ttaatcgaag aggccacgaa
     2161 gcatggaagc gtcccgtgtg cgggttttgg cttccctttc cgcgctcgcg ttttccgctg
     2221 ttgcgtttgc ggtagccggg ctttttgttg accggcagct tcaggccctt ttggacgatc
     2281 gtctggccga ggcgttgctg gcacaggcga aaaccgtcgc gggcgcggtt tttctggaaa
     2341 acacaacaga ctcggttgca ctggcgctgg caacgggtac cctgtcggcg acgcgggaac
     2401 agaccggtgc tgagttggtg gtgcttctgg acgcgcgtgg cagggttttg ttcactgacc
     2461 ctccggttct cggagagcat ccgtacatcg atctggataa cgccgcgctc gcgtccgcca
     2521 tcaccggaaa acccgcttgc ggagagcgtt tccgcatcgg gaatgtggat ctgaaaactg
     2581 ccttcgcgcc ggtgttcaac ccgttcggcg aagtagtggc gatcgtgggc atcgaagcgc
     2641 cggcagattt tttcggagca cttcgtgacg ttcggcgggc gcttgttgtt acactggggc
     2701 tgggcatggc gctcgtcgca gggttggtcg ctgtggtgtg gcatttctgg gcgcgaagtg
     2761 aggcttctga acgcgcgctg tgggatagtc agcagctggc gatgatcggg cagatgaccg
     2821 cgacgatggc gcatgaggtg cggaacccgc tcgggatcat aaaagcggcg gcagaacgga
     2881 ttcgtcgtaa gtacggcgac ggaacagagc ttttcgattt cattcctgat gaagtggatc
     2941 gactggatcg tcttaccgaa tggtatctgc gattcgccag gccgggtgat ctgaggcttg
     3001 cgaaagccag actgggtcca atcgtccggg actcgatttc ccgtgtgcgc agtgaactgg
     3061 aagcggcagc gattgaagtg gtggtggagg atctggcgca atcagaatgc ctggtggact
     3121 atgatcgcct tgtgcaggtg aacatcaaca tccttctgaa cgcccgtcag gcgattggct
     3181 caaacggaag ggtcgaggca aaagtcatcg aaaaatcggg cgtggtgagg gtggaagtga
     3241 aagacaacgg gcccgggatt ccggcctgta ttcagcccaa cgtgttccag ccgttcttta
     3301 caacgaaaac cgcgggcagc ggactgggac ttcccgttat gaaacaggtc gttgaggccc
     3361 ttggcggcag agtggggctg aacagcaggc cgggagaggg aagcacggtg tggtacgaac
     3421 tcccgtgtgc gaaaaacacc tgatcgaact gaaaaaggac gatagatgca gcgcgacaaa
     3481 gttggcaaaa tcgttcccat aggaaaaggt ctgtgtcgcg tgatcgttcg ctcacggaaa
     3541 ctggcggaaa ggctttccag tgtgcccggt gtggaggtca gagcgcaaga cgcggaatac
     3601 ctggggtggt ggataatatt cccggagtca atgcgcgcct tgatcgaacc agtattccgt
     3661 gagaaaacga tcgtgaccga acacgcctcg cagttgtcgc tgctggacat ggatgctgat
     3721 gacggtggca attctcagga ggaatcgcct cagaaatgcg gcgacggcgc aggagaagga
     3781 aatggctcgg gtactcctgg ttgacgacga acggaagctt gcggtactgc ttgctggcga
     3841 actgaccgat gcaggacact gggtgcttac cgcgcaggac ggtagaactg ccattcaact
     3901 ggtgaaggaa caggcttttg acgttgtcgt aacggatatc cgtatggagg aggttgacgg
     3961 tctcgcgctg ctcagggcgg tgaaagacac atgtccggac accgacgtaa tcatgatgac
     4021 ggcgtacgcg agtatcgaga cgacagtcga agccctgaga aagggagcgg cggactacct
     4081 gatcaagccc tttccgcccg aagaacttat cctggcagtt gagcgcgtcg tggaacgaca
     4141 gaggcttgcc ctcgagaaca gacaactcaa acgggaactc gcctccgcag aatccgaatt
     4201 catcgcggcg agcgattcca tgaaagccgt cgccgacgtc gtgtcccgga tcgcgaaaac
     4261 ggatacgagc gtgctgatca ccggcgcaag cggaacgggt aaggaagtcg ttgcaagggc
     4321 cattcacgca cggagcaaga ggaaggacgg gcccttcgtc gcggtgaact gcgcggcgtt
     4381 gccggagccg ctgctcgaga gtgagctttt tggacacgaa cgcggagcct tcaccggggc
     4441 cgaccgacgc aaactgggaa ggtttgagct ggctgacaaa ggcacgctgt tcctcgatga
     4501 gattggagaa atcggtcaaa gcattcaggt caaacttctg cgtgcgctgg agacgaagac
     4561 attcgagcgc gtaggaggga ccacgaacgt cagcgtggat gtgcggatca tcgccgccac
     4621 gaacaaagat cttcctacgg ccatcgcgaa caagcagttc cgagaggacc tgtactatcg
     4681 cctgaacgtg ttcccgataa cgctgcctcc gttgaaggaa cgggttgcgg atatccgagc
     4741 gttagcagca cattttgtgg cgccgaccgg gttgaaaatc agtgacgccg cactcaggca
     4801 acttgaggaa tacgagtggc cggggaatgt gcgtgagctc cgcaacgttc tggagcgggc
     4861 ggtcatactt tgcgacggcg atacgattct gccggaacac ttgtcgctga atcccatctc
     4921 tcgccagccg gggaacgatg gcggcgcggc agacctgaac ctggaaagaa gggaaaggaa
     4981 agccctggaa gaggcattgg cccgcgcgaa aggaaacaag gcagaggctg cgcggttgct
     5041 cggaatcagc aggagagcgc tttactcacg ggccgcgagc cttggcatta ctctgacgtg
     5101 aagaacgcac cggatggcag gtcgaacggg catgccgctg ccatgccacc cggtgcggaa
     5161 tacgctactc caacgtaatc gcctcgcttg ggcattcgtc cacacaagct ccgcactccg
     5221 tgcactcttc ggggtttacc actgcgacgt cgtcgtccat cgtgatggct tcggacgggc
     5281 acacttctac gcaactgcca caaccagtgc attcctcttt gttgactact gcaggcattt
     5341 tcttgctccc gagttgagtt gaatactccc ggaatgagtt ccgggtctgc cggctacgca
     5401 aagttcacaa gtgagctatc cccgagaacg aatctattct ctgacggcat gagtcgttcg
     5461 tgggtgatgc gaacgttctt gccaatgaga ctcgacccga tacgggattt gataccggaa
     5521 atgacgcttt tttccattat tatgctgtat tccacctcac tcccttctat cgttacaccc
     5581 ggcccgatgg aggtatacgg tcctatatat gcgtcgcgga tcgtcgaacc ggggccgaca
     5641 atgacaggcc cccgtatgat gcttctttcg acgatcgcgg aagactcgat tacgacccgc
     5701 ccgacaacgg aggactcacc gtcgacgttc ccgtggcacg aagcctccaa gattttttcc
     5761 agaagtaacc ggttcaggtc gatcatgtct tccggtctgc cggtgtcctt ccaccaccct
     5821 tccaacatca gcggctgaac cacaaggttc cgggcgatca gttcccgtag tgcatcggga
     5881 agctccagtt cacctcggag ggacggcttc acgtttccga tgacatcgaa aatgcacggc
     5941 tggaacacgt aaatcccggc gaccgcaagg ttgctttttg gtgattccgg tttttcttcg
     6001 agacctacaa ttctcccatt ttcgatttcc gctatgccaa attggcgcgg gttctccgtc
     6061 ttgtgcagaa cgacgaccga cgctggctgg ttctttgcga acgcctccac cacggaattg
     6121 atgccgttct ccacgaggat atcgccgaga accatcagaa acggtgcgcc ggctgcccag
     6181 tctcgtgcta acgatacagc atgtgctgtg ccaagaggat tatcctggac gatgtagtcc
     6241 accttgacgt cccagcgcga accgtcgccc accgcacctt cgatttcatg gcggttcacc
     6301 cccaccacta tgccgatctc cgtaattccg gcgcggtgga gcatttcaat cgtgtagtgg
     6361 atcagcggct tgttggcaac cggcaacacg tgcttcgctg aggtgtaggt gatgggacgc
     6421 aatctgcttc ccatcccggc acagaggagc aacccctttg taagcattcg gcatccttcn
     6481 nnnnnnnnng ctctcagtcg ctgagagacc tcataaccat acccgtcaga ggtttcgggc
     6541 tgaaatacgt tgatttgtgc ggcatcacat caccggcttc ggcaacctca gcaatctgag
     6601 cggccgacgg cgcgcgtatc aggaagaaga tgtcagcgga gcctgatacc acggcagagc
     6661 acccgtcctc cgggtcgacc gtatatctga tgttcatatc gttttcgctc acacccgcaa
     6721 tgcgtttcag gaccacctcg tgcaggagag acacgtcgag tttccgccag gccagcgacc
     6781 gctgcggatc tatccattcg gcaacgcgtt cccacgacct taaccggaga acaaaacccc
     6841 cgcggttctt cacccacatc ccgatctcat acggttctcc ggcacttttc ccgagcaatc
     6901 ctctgagatt cgggctggtt gcgtcaaccg ccgtcaccgt gaaatacgtt tcgagacggc
     6961 tgacaaatcc atccatgtca aacgatggcg gaaacgtcac aacccggtgc ggaggcagaa
     7021 tagtaaggtc cgggtcatcg ctggagcaaa gatacaccat cagagactcc gccgcccctc
     7081 ggatacccat gctgcgctgt tggtcgcgga accttatcgc ggtctcgtac cgatggtgcc
     7141 cgtcagcgat aataagcgga taccggcgta ctacttccgc aacgcgccgg atgtcgtcac
     7201 cgtcggttac ccgccacatc ctgcttgtgc cgtcgggggt ttcaacctct acgtcaggtg
     7261 gataactcgc cgttcgagag tacgtttcgc cgagttcgtc catcgggtcc cggtagagca
     7321 ggaacacagg actcagattg gcaagtgtct cctccataag gcggagtcgg tccgccttcg
     7381 ggcccgcgaa cgtccgttcg tgtggcagca ctgacccctt gccgatttct tccagcacaa
     7441 gttccccgat gaaaccgcgc ctcgctgaag gtctccctga gggcgtcacg taccgttcct
     7501 cgaggaaata cagcgccggg gccgggtcgc gcaccagaac tcccgactcc ctccacctgc
     7561 gccagaggct tcccgcatag cggtactttg cgtccccatc cccgatcggc aggtcaatcc
     7621 gcaccacgtt gaacaggttt tttgagagga gttgcgcacg tccttcggga ctgatgacgt
     7681 cgtacggtgg ggcaacgacg gtctgcagtg cgccaacttt ttcactgttg taacgaatac
     7741 cccgaaacgg gttgatgatc gccactaaga aacccttccc tccgcgcgtg ccttttcgat
     7801 ttcctcaaac agcgcagcca ccagttcctc ttccttcact cgccttacga ccgccccgtc
     7861 gcggtacacc agcccgacgc ctttgccacc ggcaattcca aagtctgccc cctgagactc
     7921 acccggcccg ttgaccacac accccataac cgcgacccgg aatgattccg agtacttctc
     7981 cagacggcgc tccacttcct gagctattct gatcaggtcg atttccacgc gcccgcatgt
     8041 ggggcacgaa atcagcgtcg ggcctttcac tctcaggcgc agcgatttca ggatatcgaa
     8101 cccgacggca acttccttga ccgggtcatc ggtgagagac acgcgtatcg tatccccgat
     8161 gccggccgac aggatagctc cgatgccgac tgccgaagcg atggtacccg ttttcgcggt
     8221 tcccgcttct gtgatgccca cgtgcagagg atatgggcgc tctctggaaa agtcggtata
     8281 cgccgcaatc gccatcggaa cgtccgacgc cttgagcgat accacgatgt cgtaaaatga
     8341 cagatcctca agtattcgga cgtgccgcag cgcgctttct accagtccag cggccgtcgg
     8401 gtacccgaat ttctccagga tgtccttttc cagcgaacct gcgttaactc caatcctgat
     8461 cgggatcccg cgctctgagc atgcgctcgc cacgcgttcg acagattgcc gcgtaccgat
     8521 gttgcccggg ttgatacgaa gcttgtccac tccctgctcc accgccacca gcgcgagacg
     8581 gtgtccaaaa tgaatgtcgg cgacgagagg tatcggaatc tgtcgacgaa tttccccgag
     8641 cgcacgcgcg tctgcttcat gcggcaccgc aaccctgaca atttcgcaac cggcgtccgc
     8701 aagggcgtga atctgtcgga cggtagcgtc tatgtctcgc gtgttcgtgg tgcacatgga
     8761 ctgaaccgtc accggcgcac caccgccaat ttgcactccg ccgaccgaaa cgcgacgggt
     8821 tgcggtccga agggtaagcg gcttcacgtg agaagaggtg tcgttaccta accccgcgga
     8881 accagggttc tcgtgtgcca tggaacgtgt ccgtgaaaaa cgcagaaatt caactctgga
     8941 aaacgcgctt cgggaatgcc gcgacgcagt gcccgggcgg gaaagtaata agataagaca
     9001 cttcgcccgg ctccgtccat ttcccgaaat ggtttcgctt ctcagaacac cgcattgaat
     9061 tccgcccttg cagtgtgcgt caatcgtgtt cctgcatttc gacgggcgga ataggagaca
     9121 gacagtgtgg ctagagatga aaggcgatag ctggccagca ttgatgcacg gaaattgtgg
     9181 cccccgttcc tgccgttgag caggtggacc ggcagcattc cgccgtgcgt gccattccgc
     9241 acaaaaactc ccgaccattg tgcggaggta cgcatcacgc cacgatttcc ccacgatttg
     9301 cgccacgccg gttcaaggct gatgacgtac gcttccagcg gcttgccctg aacagccacg
     9361 tacgccgcgg cgggaacacg atccttgcct tcacccgctt cgttccgcaa cgagaattca
     9421 tcgaacgacg aattccggta cgtgatagat tgatcgagcc agttcctccg cacggagtaa
     9481 tcctggttgc tcccgttcct cagatcgctc tgccatttga gctctgcggt ccaatccgtc
     9541 ccggggcgca ccctgagtct tccacgaacc gagtagtctt tctggttcgt cactgccggc
     9601 atgccggcgc gggaatacaa tgcagcgtca taatcttcat acgtcataat tcggaaccgc
     9661 aaatctcccg cccacgaggg gtcccggaga agcaagtcct gggtaactcg caccgaaccc
     9721 cgcagggtcg gagacttccc tggtttgcga aacgtccata gctttaaaag gtaaagatca
     9781 ctcgtacgcg aagtgtcggt ctcactgatc agaacgttcg tctctgtacc caccaacctg
     9841 atcgctcgtt gccacaacgg acgaatctcg tttgtccgcg gattcgccgc tcctttccag
     9901 aaacggttga aatccgtgtt caactgccac gccgcgtcca gtgtgaccgt aggactgaag
     9961 tcgtcggtgt cccggtaccg gagaacgtaa gaagcggttg gatcgccggc aggcgcgatg
    10021 acgacttctg tttcagcgag gatcgcccgg gtgtcgttcg ggtcaatggg tgcgtagtcg
    10081 ccgcggccgg caccaacata gagaaacaga cgctcacgct ttgccgttcg agtggaagcg
    10141 atacggtagc gaaacgcatt tcgtacgccg ccgccgagag catcatggcg gatatccacg
    10201 tcagcattgt ccgaaacggt gctgcccccg ccggcgagat tctgacgttt tcgccaccca
    10261 tattccaccg tactggaaag acctttccag gagcgcacgg cgccgcgcca cgtctgcgtg
    10321 tacgatctct gctggcggac ccagctcgcg aacagactgt cgtacgcgct gttgtaccgc
    10381 gattggacct ccgtggaaat cgcgaaatgt tctgtctcgg agatcgcaag tccggccgac
    10441 cagtcctgga acctcgttcc gtagagacgc tcgcgagggc tgtcccgcgt atcctgttca
    10501 ccttcccatt tcgccgatga agttacccgg aaaacggggg ctcggacttc cgcgtggccc
    10561 cggaaaacat caccggatgt
//
LOCUS       DAOTNG010000078        10553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_5901_length_10553_cov_5.116213, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000078 DAOTNG010000000
VERSION     DAOTNG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5901_length_10553_cov_5.116213"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="P5179_05360"
     CDS             complement(<1..969)
                     /locus_tag="P5179_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRS94679.1"
                     /translation="MGALRFGDLLAIVLYAVAMLWLGWWSQKRIKGTEGYFVGNRSLP
                     GWAVGLSILATAISSITFLAYPGNSYQGNWSRLVPGLMLPVATLIGVYFFVVFYRRTL
                     FVSAYQYFEQRFGNWGRSYASVLFTLGSLYRMGMVLYLLSIPIKVMFGLDISTTILFA
                     GIIVTIYTVMGGLEAVIWTDVAQGIILIAGGVITVLIAFIDVPGGAHEIISRGMAQGK
                     FDMAVSFDWDFTKETIWIFMLMGLVGNIQELSTDQTKVQRYQAAKSDKGAIGATLTVL
                     ACIPVWMLFMFVGTCLWAYYTDFPQLLPAGLKADYVYPHFILTQMPT"
     gene            1206..4544
                     /locus_tag="P5179_05365"
     CDS             1206..4544
                     /locus_tag="P5179_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94680.1"
                     /translation="MNTPADTLRTILSRVRAERRRAAALTLALATGAVGLAGLLAAVL
                     LETVGAFPPVGRLALLGGWVGTTAFAASFIFSRVRRAVGTHETIARDIEHVYPEFNER
                     LIAGWEFATRPVSGSRSLADSVVREAALVLEKVDLRPLISWRPVARSAGAFFVTFAVV
                     CVSWFAFPDQVPSAFNRLVHPTVQFVTPPPFAFTLSPDNTRLVRGDTLTVKVRFSGSY
                     PRSAVVFSRDLQTQAWRSDIVSVGDSALLIWQALQLRSDIEYHVVAGKLESPTYRVTV
                     VNPPAVERFRVVLTYPGYTGMSPDTLPENEGNIAALTGTVASFAVHPSKPVQRGELRF
                     FGRDTAVVALRKEGNVLAANWTVRHSGDYTIHLVDEFGYSDRNPIAFRITTVADEYPS
                     VRITEPEQESDLGADMLVPLVVEARDDFGFDRAELVYRGPDGRPKTLRLPLTTLERGR
                     AEVHYTWDVGELDLLPEERVYYRVAVYDNDRISGPKRVESEEQVLRFPSAAEVFAEAQ
                     QQQQQAIVDLSAITRQADEVARQLEAARRELLKNPSLSWETRQASQRALQQQRELNRE
                     LQAVADSLRQSLERLQKHDILAPETMEKLVRIQEMMEDIVTPELRQTLSELQNSLQQM
                     ADPRQVQQALQRFAQSREQFEERLDRTLRLLQEAQAEQQLDAMSKRLRELARMQRDVA
                     ESFDRQARETLANREQILGGQVSETQDRLERMAEEMRGLPASPSDSLRSVAEQLDRNR
                     IAERLQQVGNRLAQGGEPARAQNKGEAARLAEDLERTAGAMQRTAEARRNARKREIAA
                     QLDRAATDLLRLSMMQEDLRNRTRQRRGSAGASREGEEQVDLLQGTSGVIRRVMEASQ
                     ETFLIPPETLQELGTALIEMKGASEALEQNNATSAVPRQQNAMASLNRAVDMIRSAQR
                     NAQTGESGTGLEELLRQLGEAARRQKQLNDAMQALGQQPSLSPSDLQMLGQMAAEQRA
                     LSQLMQQLAEALKRHREVLGRLGDLAGEMESAAEEMERRQLGPRLADRQQRILQRLLD
                     AQRSLQQDKVSEQRVARTAGHQPLRPPGALPEDLGERKAALREALLDALKADFPPEYR
                     TWIRSYYEWLMGREGRESAPQSAP"
     gene            4719..5189
                     /locus_tag="P5179_05370"
     CDS             4719..5189
                     /locus_tag="P5179_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRS94681.1"
                     /translation="MKLCDLLTDSAVAVALPAKDKSDLLRKLVDLATKSGSVQDPAGA
                     LHAVEEREKIMTTGIGHGVAIPHGKTNAVRDLVAAFAVTEEGVDFGSLDGEPVRLAFI
                     LLGPKEPSGPHIRMLSRISRLMNREDLRAKLINAKSVAEVRALFTDAEHDMFDL"
     gene            5327..8410
                     /locus_tag="P5179_05375"
     CDS             5327..8410
                     /locus_tag="P5179_05375"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS94682.1"
                     /translation="MEIQAAKERFVSPPATNRPVIFWFWNDHLHPERLVWQYDRLIEA
                     GMAGAVMHARGGLESAEYLDERWFAAVDAVVKHAAEKGTVVWIYDELGWPSGSAGGRA
                     LVGLPREMQLGHLKLFDITPEPGKDYSRLPGSPVAAFRVVRSDPDHGFQRRYDKSVNL
                     MRDEITCEELPKDVAPSAFVGTRLLLFRHVIDGGLIDYYNAAATEVFLKSTHEEYFRR
                     FGAHFGTTITHSFMDEAGSMAGIGMLPWSPVFESEFEKRRGYAIRPHLAALLFEIPGF
                     QSVRYDYWSLTAELFREGFGVPMHQWCERHGIKYSGHYVFETSLKEATRQLGSAMPLY
                     EFQGLPGVDVLGNDFYTHRFEIEAHAYYTAMVKQASSVSNQLRDGQLLSESYGVGGHA
                     MGLESMQVATFWQMALGVTVISQHAAFYSMRAERKEDCPPVIGWQEPYWKFARPHIDS
                     ISRTSWLLSQGRRRCNVLYLHPQAGMQASYRQCRVKDEYKYESYILNADMPYEMIDKH
                     FSLLGVALLDAQIDFEYGDEEILAAHGVAEYGAFKVGKQTYETVLVQPMLNIRSSTLA
                     LLEAFAAQGGQIVLVGSAPGFVDGKSSRKALDFFSAHARRVTEGVDFFDYAPAVEVLS
                     ALGCRTVETSSPVPQIKVHRRLWDGRDIVFLANISRRTVETEVTFVPQVTGTVEEWNV
                     ADGTTHPVAHCTAGQPLTLAVKWHPKQARTFVTMEGAVQLPPVVTWSEERRLTPQWAG
                     TRTGPNALLLDACVVQDLSGASRLLSVGDARRTVLAAQMKNGSGDKPFAARFPVRVSE
                     TDPPRSACELAIELGTGPRLAFNGVPVPVNTLGWVIDPAIERIALPGLNPGENTLFVE
                     AAYGKATEFETPWLLGDFAVRTEDNVSFTVEKAAQTVPIGPWHELGLPFYAGTVVYRA
                     EVELELSDAQRIVVDLAGLAGSAGVSVNGQFVTTVLWPPYECDITSAVKPGTNVIEIE
                     VANTLRNLLGAHYVENEEIRTGAPTNLYTAPFGTPKKFLPYGLLTAPEIVISRCS"
     gene            8430..>10553
                     /locus_tag="P5179_05380"
     CDS             8430..>10553
                     /locus_tag="P5179_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRS94683.1"
                     /translation="MVRNWMPLAHGWKFLRDECSHAWKADFDDSLWETVSVPHSFNAA
                     DTFVPEPGYPDYYRGGTWYRRILPRTPAGARVELTALGVFSVTDVWVNGKHVGKFMGG
                     YTGFCTNLTPYLKRAGENVLALRVTNQHNPDVLPGLDFPDYNLYGGIYREIGLTITAP
                     LHIPDRGIVLTTPRVSRESAAIHLNVRVANDQKRSASGTVVVDVKSPGGRVVARGRQH
                     YVVAAHTERMVTIPLPQISQPKLWSPDSPALYSVHVKLLEGDEVVDAQSLRFGFRWFC
                     FDADKGFFLNGRHLKLHGVNRHQDFAGLGNALPANFQAHDVKLIKEMGANFVRCSHYP
                     MHPAFLDACDEMGVIVLEEIASWQWIGGSRFTENAIAMMREMIARDRHHPSIILWGLL
                     NEGRSSDLFRTLNATAKQADPWRATIYADNEPEEGLELGTVFVPDVLGLNYKVPHLDD
                     LRAILKGIKLSNTEHTNANTGERRKPDGTLFSDEESQIWQIERILHDIAEFEKREWIA
                     GNALWCMHDYGTEYAVSRPIQKSGVFDAWRIPKAAAYAIRAWWSKEPFVRIAEHWTYP
                     GTEGSPRKVIVLSNCESVELLVNGRSFGSRTRDPHAYWFEWEVPYSPGEIVAVGHTPS
                     GDLRDLRRTAGAPSAMVIQTLDESLPANGTDITLVTVSVVDVHGTPVPAAEAEVRFSV
                     AGGGTFFGLYGNASVSTRNGVGRIAY"
BASE COUNT         2195 a         2973 c         3331 g         2054 t
ORIGIN      
        1 ggtcggcatc tgcgtgagaa tgaaatgggg atacacgtaa tcggctttca gtcctgccgg
       61 aagaagctgt ggaaaatcag tgtagtacgc ccagaggcag gtgcccacga acatgaacag
      121 catccagacg ggaatgcacg cgagcacggt gagagttgct ccgatggctc ctttgtccga
      181 tttcgccgcc tgataccgct gcactttcgt ctgatcggtg gaaagttcct ggatgtttcc
      241 cacaaggccc atgagcatga aaatccagat ggtctctttc gtgaagtccc agtcgaagga
      301 tacggccatg tcgaacttgc cctgcgccat ccctcgggat atgatctcgt gagcgccacc
      361 cgggacgtcg atgaacgcga tcaataccgt aatcactccg cccgcaatca ggatgattcc
      421 ctgtgcgacg tccgtccaga tcaccgcctc aaggccgccc attaccgtgt agatcgtcac
      481 aatgatgccg gcgaacagga tggtggtgga aatgtcgagg ccgaacatga ctttgatggg
      541 gatcgaaagc aggtaaagga ccatccccat gcggtacagc gaaccgagcg tgaacagcac
      601 gctcgcgtaa gaccttcccc agttcccgaa ccgctgttca aaatactgat acgcgctcac
      661 gaagagcgtc cgtcggtaga acacgacgaa gaaatacacg ccgatgagcg tggcaacggg
      721 caacatgagg ccaggaacaa gccgcgacca gttgccctgg taactgttgc cggggtacgc
      781 gagaaacgtg atggaactga tagcagtggc aagtattgaa agacccaccg cccagccggg
      841 cagagatcta tttccgacga agtaaccttc tgtgcccttg atgcgctttt gcgaccacca
      901 accaagccac aacatggcaa ccgcgtacag cacgattgcc agcaaatctc cgaatcgtaa
      961 cgcccccata cgcgtacctc cgcccgcaaa ggttcgttgt gtgtgatcgt cagaacgaga
     1021 aaaatgaaag atgaatatag gccttttcgc tctggagggc atacccttcc tacggagggg
     1081 tacccttcgg gtatcaccac ggcgacgcgt cccaaaggca ttgactctgc gttcgttgga
     1141 accacctccg ctgccgggct attgtgtaca cagggcaagt ttgtgcacga cgcgacggaa
     1201 ccatgatgaa cactcccgct gatacccttc gtacgattct gtcccgtgtt cgcgcggaac
     1261 ggaggcgtgc ggctgcgttg acactggcgc tggccacggg cgctgtcggc ctggcagggc
     1321 tgctggctgc ggtgcttctt gagacggtgg gcgcatttcc gcccgtgggt cgtctcgcgt
     1381 tgcttggtgg atgggttggt acgacggctt ttgcagcctc gttcatcttt tcgcgagtac
     1441 gacgggcggt gggaacgcac gagacaatcg cgcgcgacat cgaacacgtt tatccggagt
     1501 tcaatgaacg attgatcgcg ggctgggagt tcgcgacgcg tccggtttcc gggtcgcgga
     1561 gcctcgccga ctcggtagta cgtgaagccg cgctcgtgct tgagaaagtt gatcttcgcc
     1621 cgctgatttc gtggcgcccg gtggcgcgtt ccgcgggtgc gtttttcgtc acctttgcgg
     1681 tggtttgcgt ttcgtggttc gccttcccgg atcaggtacc ctccgcgttc aaccggttgg
     1741 tccatccgac ggtgcagttt gttacgccgc ccccgttcgc cttcacgctg agtcccgaca
     1801 acacgcggct ggttcgcggc gatacgctga ccgtgaaggt gcgtttcagc gggtcgtacc
     1861 cccggagtgc ggtagtgttt tcgcgtgacc ttcagacgca ggcatggcga agcgatatcg
     1921 tcagtgtggg agactccgct ctgctcattt ggcaggcgct gcagttgcgg tcggacatcg
     1981 aataccatgt tgtggcggga aaactggaat ctccgacata tcgagtaacc gtggtgaacc
     2041 ctcccgcggt ggagcgcttc cgcgttgttc tcacctaccc gggttacacg ggaatgagcc
     2101 ctgatacgct gccggaaaac gagggtaaca ttgccgctct gacaggtacc gtcgcgtcgt
     2161 ttgcggtgca cccgagcaaa ccggtccagc ggggagaatt gcgtttcttc gggcgagaca
     2221 ccgcggtggt ggcgcttcga aaagagggca acgttctcgc cgccaactgg acggtacgac
     2281 actcgggtga ctacacgatt cacctcgtgg atgagttcgg gtactcagac cgcaatccga
     2341 ttgcattccg tatcaccacg gtggctgacg agtatccctc tgtgcgcatt acggaaccag
     2401 aacaggaatc agatctgggc gcggacatgc tggttcccct cgtggtagaa gcgcgcgacg
     2461 atttcgggtt cgatcgtgcc gaactcgtgt accgcggccc cgacggtaga ccgaagaccc
     2521 tgcgcctgcc ccttaccacg ctggagcggg gacgcgcgga agtgcattat acatgggacg
     2581 tcggcgaact ggacctgctg cctgaggaac gggtgtatta tcgtgtggcg gtctatgata
     2641 acgaccgcat atcagggccg aaacgagtgg aaagcgagga gcaggttttg cgatttccca
     2701 gcgccgctga ggtattcgcg gaggctcagc agcaacagca acaggcgatt gtggatctga
     2761 gtgcgattac gcggcaggcg gacgaggtag cacggcaact tgaggcggcg cgccgggagc
     2821 ttctgaaaaa tccgagcctt tcatgggaga ctcgccaggc atcgcagcgt gccctgcaac
     2881 aacaacgcga actgaacagg gaattgcagg cggttgcaga ctcgttgcgg caatctctgg
     2941 agcggctcca gaaacacgat attctcgctc cggagaccat ggaaaagctc gtgcgcatcc
     3001 aggaaatgat ggaggacatc gttacgccgg agcttcgtca gacgttgtcc gagctccaga
     3061 attcgctcca acagatggcg gatccgcgtc aggtgcaaca ggcgctgcaa cgattcgcgc
     3121 agagccgcga gcagtttgag gagcggcttg accggaccct caggctcctg caagaggccc
     3181 aggcggaaca gcagctcgac gccatgagca agcgactgcg ggagcttgca cgaatgcagc
     3241 gggacgttgc ggaatcgttt gaccggcagg cgcgggagac gcttgccaac cgggagcaga
     3301 tactcggcgg gcaggtttca gaaacgcagg accggctgga acgcatggcg gaggagatgc
     3361 gcggcctgcc tgcgtctcct tctgatagtc ttcgatcggt tgccgaacaa ctggaccgga
     3421 acaggattgc agaacgcctc cagcaggtcg gaaacaggct ggcgcagggg ggagaaccgg
     3481 cgcgtgcgca gaacaaaggt gaagcggcgc ggctggcgga agatctcgaa cggacagccg
     3541 gagccatgca gcgcaccgct gaagcgcggc ggaacgcgcg caaacgggaa atcgcggcgc
     3601 aacttgaccg ggccgcgacg gaccttctga ggctttccat gatgcaggag gatctccgga
     3661 accgcacgcg ccagcgcaga ggcagtgccg gtgcttcccg tgagggcgag gagcaggttg
     3721 accttctgca gggaacatcg ggggtgatac gccgtgtgat ggaagcgtct caggagacat
     3781 tcctgatccc tccggaaaca ttgcaggaac tgggcaccgc gctgattgag atgaaaggtg
     3841 caagcgaagc actcgaacag aacaatgcga cctctgcagt cccacgccag cagaatgcga
     3901 tggcatcgct caacagggcg gtagacatga tccgttcggc acaacggaac gcgcagactg
     3961 gcgaatcggg gacaggtctg gaagagcttc tgcgccaact tggagaggcg gcccgtcgcc
     4021 agaagcagtt gaatgacgcg atgcaggcgc tgggacaaca gccgtcgctg tcaccgtcag
     4081 acctccagat gcttggccag atggcagccg agcaacgggc gctcagccag ctcatgcagc
     4141 agcttgcgga ggccctgaaa cgccacaggg aggttctcgg acggcttggg gatcttgcgg
     4201 gtgagatgga aagcgcggcg gaggagatgg agcggcggca gctcgggcct cgtctcgcgg
     4261 atcggcaaca gcgcatcctc cagcgtctgc ttgacgcgca gcgttcgctg cagcaggata
     4321 aggtgagcga gcaacgggtt gccaggacgg cgggccacca gccgctccgt ccgccaggcg
     4381 ccttaccgga agacctcggc gagagaaaag ccgctctgcg ggaagcgctc ctcgacgcgc
     4441 tgaaagcgga cttcccgccg gagtaccgga cgtggatccg gtcgtactat gaatggctca
     4501 tgggccgcga ggggcgcgaa agtgcgccac aatcggcacc ctgatgagcg gcggcgaggg
     4561 gggtatggca tataacactg gccgagtgtt attttgtaca ttcccattca cccccttggc
     4621 tttccggctg cacgccgtat cgggagtgac gaagcgacaa acccggaggc aggtgcgcaa
     4681 cctttggaca agtgccgtgg aaaggcagga attatcggat gaaactttgc gacttactga
     4741 ctgactcggc tgtcgctgtt gcgctgccag cgaaggacaa gagcgatctc ttgcggaaac
     4801 ttgttgatct ggcaacgaaa agcggttccg ttcaggaccc ggctggtgcg ctgcatgccg
     4861 tggaggaacg ggagaaaatc atgacgaccg ggataggtca cggtgtcgca attccccacg
     4921 gaaagaccaa cgccgtgcgc gatcttgtcg ctgcgttcgc ggtgacggaa gagggagtgg
     4981 attttggttc gctggatggc gaaccggtga ggctcgcgtt cattcttctg gggccgaagg
     5041 agccgagcgg tccccacatt cggatgctga gccggatatc acgcctcatg aaccgggagg
     5101 acctgcgcgc aaagctcatc aatgcgaaat cggtcgcgga agtgcgcgcc ctgttcaccg
     5161 atgcggaaca cgacatgttc gatctttgaa ggttgaaaag aataaaggca atcggggtcg
     5221 tgcggtgcag gcgtagcagc acggcccctt gtgtttttcc cgccctcctc ccgttgtttc
     5281 tttcgagaca atcctcacgt gcaaaacccg ttgaaagggt tcttccatgg aaattcaggc
     5341 ggcaaaagaa cgctttgtct cccctccggc cacgaaccgc ccggtcatct tctggttctg
     5401 gaacgatcac ctgcaccccg agaggctcgt ctggcagtac gaccgcctca tcgaagccgg
     5461 catggcggga gcagtaatgc atgccagagg gggactcgaa agtgcggaat acctcgatga
     5521 gcgctggttc gccgcggtgg atgcggttgt caagcatgcc gccgaaaagg gtaccgtcgt
     5581 ctggatctat gacgaactgg gctggccatc cggaagcgca ggcgggcgcg cacttgttgg
     5641 ccttccccgg gagatgcagc ttggccacct caagctgttc gacattacac cggagcccgg
     5701 aaaagactac tcgcgccttc ctggaagccc ggtagccgcg ttccgagttg tacggtcaga
     5761 tcctgaccac ggattccagc ggaggtatga caagagcgtc aacctcatgc gggacgaaat
     5821 tacgtgtgag gaactcccaa aggacgttgc cccatctgca ttcgtgggga cgcggcttct
     5881 cctgttccgt cacgtgatcg acggcggcct gattgactac tataacgcgg ccgcaacgga
     5941 agtcttcctc aaatcgaccc acgaggagta cttccggaga ttcggcgccc acttcgggac
     6001 aaccatcacc cacagcttca tggacgaggc cgggagcatg gcgggtatcg ggatgttgcc
     6061 atggagcccg gtgtttgagt cggaatttga gaaacgccgt gggtatgcga ttcgtcccca
     6121 tctcgccgcg ttgttgtttg aaatccccgg cttccagtcg gtccgttatg actactggtc
     6181 gctgacggct gagcttttcc gcgagggctt tggggttccg atgcaccagt ggtgtgaacg
     6241 gcacgggatc aagtacagcg gccactacgt gttcgagacg tcgttgaaag aagcgacgcg
     6301 gcagcttggc agcgcgatgc cgttgtatga gttccagggc cttccggggg tggacgttct
     6361 tgggaatgat ttctacacgc atcgttttga gattgaagcg catgcgtatt acaccgcaat
     6421 ggtgaaacag gcttcgtcgg tttccaacca gcttcgggat ggccagctcc tgagcgagag
     6481 ctacggcgtg ggcggccacg cgatggggct cgaaagcatg caggtggcca ccttctggca
     6541 gatggcgctc ggggtaacag tcatcagcca gcatgccgcg ttctacagca tgcgcgccga
     6601 acggaaggag gattgcccgc cggttatcgg ctggcaggaa ccttactgga aattcgcccg
     6661 cccgcacatt gattcaatca gtcgcacgag ctggcttctt tcgcaggggc gccgtcgctg
     6721 taacgttctt tacctgcacc cgcaggcggg aatgcaggcg agttaccggc aatgccgcgt
     6781 caaggacgag tacaagtacg agagctacat cctgaacgcg gacatgccgt acgagatgat
     6841 cgacaagcat ttttccttgc tgggcgttgc tctgctggac gcgcaaattg atttcgaata
     6901 cggcgacgaa gagattctgg ccgcacacgg cgttgcggag tatggggcgt tcaaggtagg
     6961 aaagcagacg tacgagacgg ttctggtgca accgatgctg aatatccgtt cgtcgacgct
     7021 tgctcttctg gaagcctttg ccgcgcaggg agggcagatc gttctggtag ggtcggctcc
     7081 cggctttgtg gatgggaagt cctcgcggaa ggcgctggat ttcttctccg ctcatgcccg
     7141 gcgagttacc gagggcgttg atttcttcga ctacgcgccc gcggtggaag tgctttctgc
     7201 gctcgggtgc cgcaccgtgg aaaccagttc tcccgttccc cagatcaaag tccatcgccg
     7261 cctctgggat ggcagggata tcgtgttcct cgcgaacatc tcccgccgca cggtggaaac
     7321 ggaagtcacg ttcgttccgc aggtgacggg cactgttgag gaatggaatg ttgccgacgg
     7381 caccacacac cccgtggcgc actgtacggc gggccaaccg ctcactctgg cagtgaaatg
     7441 gcatccaaaa caggcgcgga ccttcgtaac gatggaaggt gcggtgcaac tcccgcccgt
     7501 ggttacgtgg agcgaagaac ggcgcctgac tccgcagtgg gcgggaactc gaaccggccc
     7561 gaatgctctg cttctggatg cctgtgtcgt tcaggatttg tccggcgctt cgcgcctgtt
     7621 gtccgtgggc gacgcaagaa ggaccgtcct tgccgcgcag atgaagaatg gctcgggcga
     7681 caagcccttc gcagccaggt ttccggtgcg cgtttcggaa accgacccac cgcgttcggc
     7741 gtgcgaactt gccatcgagc ttggaaccgg accgcgtctg gcatttaacg gcgtaccggt
     7801 tcccgtgaac accctcggct gggtgataga cccggctatc gaacgaatcg ccctgcccgg
     7861 gctcaacccc ggtgagaaca cgcttttcgt cgaagctgcg tacggaaagg caacggaatt
     7921 cgagactccg tggcttctcg gggatttcgc cgtccggacc gaggacaacg tgagtttcac
     7981 cgtggaaaag gcggcacaga cagttccaat cggtccatgg cacgagctcg gtctgccatt
     8041 ctacgcgggg acagttgtct atcgcgctga ggtagagctc gagctttcgg atgcgcagcg
     8101 aatcgtggta gatctggcgg gcctggccgg ttcggcgggg gtcagtgtga acggccagtt
     8161 cgtcaccacc gtgctgtggc ccccgtatga gtgcgacatt acctcggcgg tgaagccggg
     8221 cacaaatgtt attgagatag aggtggcaaa cacgttgcgt aacctcctcg gtgcgcacta
     8281 tgtcgagaat gaggaaatca gaacgggggc gccgacaaat ctgtacaccg cgcctttcgg
     8341 aacgccgaag aagttccttc cctacgggct tctcaccgcg ccggaaatcg tgatttcgag
     8401 atgcagttga cagcatgggg gtgatacaca tggttcggaa ctggatgcct ctggctcacg
     8461 ggtggaaatt cctgcgggat gagtgctcac acgcctggaa agccgatttt gatgattctc
     8521 tgtgggaaac ggtttcggtc cctcactcgt tcaacgccgc cgatacgttc gtgccggaac
     8581 ccgggtaccc ggattactac cgcggaggaa cctggtatcg caggattctg ccgcgaacac
     8641 ccgccggtgc gcgcgtcgag ctcaccgcgc tgggagtgtt ttcggtaaca gatgtgtggg
     8701 tcaacgggaa acacgtcgga aagttcatgg gagggtatac cggtttctgc acgaacctga
     8761 caccgtacct caaacgagcc ggcgaaaacg tgctcgccct ccgggtgacg aatcagcaca
     8821 accccgacgt tctgccgggt ctggatttcc ccgattacaa cctttacggt ggcatttacc
     8881 gtgagattgg tctcaccatc acggctcctc tccacattcc cgatcgcggc attgttctca
     8941 cgacgccacg ggtctcgcga gagtcagccg cgatccacct taacgtccgc gtcgcgaacg
     9001 atcaaaaacg ctccgcttcg ggcactgtgg tagtggatgt aaaatctccc ggcggacgcg
     9061 tcgtcgcacg tgggagacag cactatgtgg tggccgcaca cacggagcgt atggttacca
     9121 tcccgctgcc gcagatatcg caaccgaagc tctggtcacc ggatagcccg gcgctgtatt
     9181 cagtccacgt gaaactcctg gagggggacg aagtagtaga tgctcagtcc ctccgctttg
     9241 gattcagatg gttctgtttc gacgcggata aagggttctt cctgaatggc aggcacctca
     9301 aactgcacgg tgtgaaccgg catcaggact tcgccggtct gggaaacgca cttccggcga
     9361 atttccaggc gcacgatgtg aagctgatca aagaaatggg tgcgaacttc gtgcgatgca
     9421 gccactatcc catgcatccg gcgtttctgg atgcatgcga tgagatgggg gttattgtgc
     9481 tggaggagat cgcctcgtgg caatggatag gtggaagcag gttcaccgaa aacgccatcg
     9541 ccatgatgcg cgagatgatc gcgcgcgaca ggcaccaccc gtcaatcatc ctctggggcc
     9601 tcctcaacga aggccgcagc agcgatctgt tccgtactct gaacgcgacc gcgaagcagg
     9661 cggacccgtg gcgggctacc atctatgccg acaacgaacc ggaagagggt cttgagctcg
     9721 gaacggtatt cgtgccggat gtccttggtc tgaattacaa agtgccgcat ctggatgacc
     9781 tgcgcgcgat tctcaagggc atcaagctct ccaatacgga acacacgaac gcgaataccg
     9841 gtgagcgcag aaagcccgat gggacgctct tttccgatga agagagccag atctggcaaa
     9901 tcgagcgcat actgcatgac atcgcggagt ttgagaagcg ggaatggata gcgggcaacg
     9961 ctctctggtg tatgcacgat tacggtacgg aatacgcagt ttcacggccc atacagaaat
    10021 cgggggtgtt cgacgcgtgg agaattccaa aagcggcggc atacgcgatc agagcctggt
    10081 ggtcgaaaga accgtttgtg cgcattgccg agcactggac gtaccccggc accgagggca
    10141 gtccccgcaa ggtgatcgtg ctgagcaact gtgaatcggt cgaactgctc gtgaacgggc
    10201 ggtccttcgg gagcagaaca cgggacccgc acgcgtactg gttcgaatgg gaggtgccct
    10261 attcccccgg cgaaatcgtt gcggtaggtc acactccttc aggggacctc cgggatttgc
    10321 gccgtacggc gggagctccc tccgcaatgg ttatacagac gctcgacgag agcctccccg
    10381 cgaacgggac agacatcaca ctggttactg tgtcggtcgt tgacgtacac gggactccgg
    10441 ttccggcagc ggaagccgag gtacggttct cggtggcagg gggcgggaca tttttcggcc
    10501 tgtacggcaa cgcgagcgtc tcaacgcgaa atggagtcgg acgcatcgcg tac
//
LOCUS       DAOTNG010000079        10350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6034_length_10350_cov_5.710733, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000079 DAOTNG010000000
VERSION     DAOTNG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_10350_cov_5.710733"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            708..878
                     /locus_tag="P5179_05385"
     CDS             708..878
                     /locus_tag="P5179_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94684.1"
                     /translation="MLGIADPQIWLAYVLCILSTILCVYYGAKNWDKGDEPVYPEDVT
                     WAEDEKKATAEN"
     gene            954..2555
                     /locus_tag="P5179_05390"
     CDS             954..2555
                     /locus_tag="P5179_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRS94685.1"
                     /translation="MDNFAILTVIVVVYLFVVAYLGYYGYRKTKNATDYLVAGRSTHP
                     YIMALSYGATFISTSAIVGFGGAAALFGMGLLWLTFLNIFFGIFIAFVVFGKRTRKIG
                     RNLDAHTFPEFLGRRFDSRFIQGFAGITIFLLMPLYASAVLIGAARFIENTFAGVNYN
                     TAILIYTLIICAYVLAGGLKGVMYTDALQGTIMFVGMLVLAVYTYSTLGGVIDSHQAL
                     TNMADKVPAKLAAIGHQGWTSMPALGSPLWWQLVSTIVLGVGIGVLAQPQLVVRFMTV
                     KSSRELNRAVLSGGIFILAMTGVAFVVGALSNVFFYEHTAYKAISLAVAKGNIDSIIP
                     LYVNSAMPVWFVYLFMITLLSAAMSTSSSQFHTMGSAFGRDFFEKTLFRGKQFLYTVS
                     LTRIGIVLGVIATVILGYNLPGSVIAIATAIFFGFCATMFLPSFVGALFWKGMTRAGA
                     VSSVVVGFFGSACWLLFVHQKESEALGLCKLVFGKASLAAGTVWAMVDPIVVILPISF
                     VVAFGVSMWTKKMPQEHLDACFKHM"
     gene            complement(2754..>2963)
                     /locus_tag="P5179_05395"
     CDS             complement(2754..>2963)
                     /locus_tag="P5179_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRS94686.1"
                     /translation="VAIALGYWKWEQNCIPLQNYAAWFFVSLLMSAAYVHLRVTWRNP
                     APFIIVFAQLVFFAVLRVSLGLSAC"
     assembly_gap    2964..3063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3092..4141)
                     /locus_tag="P5179_05400"
     CDS             complement(3092..4141)
                     /locus_tag="P5179_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS94687.1"
                     /translation="MNAGYFGSRSEEIKAFLRSHLETCVEEFSFLNRWGKDLPARIGA
                     FARGGKFIRGGLVFLGHELAGGRPGETTLKIAAAIELLHSAFLIHDDIMDQDRMRRGM
                     PSVFWQFREEAHAKGIEESEHYGTSMGICAGDCALFIALRLISSADIPDGVKDLVCRE
                     SYAIGLAQMADIDYGTFSRIPSEKEIIDLYLWKTARYSFSLPLSAGAMAGNADEELVS
                     ALWRLGESMGIMFQMRDDELGIFGSENAIGKPVGSDIRLAKKTLLIAIALKRLDPVSR
                     SELTGILGRRDATADEILRARRLIETSGARSELISRMNALAAETGKMIDALVAPEDSK
                     ERLRSLVDFIRTRDR"
     gene            complement(4138..5004)
                     /locus_tag="P5179_05405"
     CDS             complement(4138..5004)
                     /locus_tag="P5179_05405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRS94688.1"
                     /translation="MKRTQSAFEEIFRRGSRTFFNSSLFFPEKVRRDVVILYAFVRTA
                     DDFVDSIPQDKEGFHAFRARYELARRTGRDSGDPIADSFIELSRRRKFDDAWAEAFLN
                     SMEADLTRKKYETPDEVLVYIHGSAEVIGFFMASIMELPPNAMEAAAFLGRAMQYINF
                     IRDIAEDNELGRTYLPLAGSGLEDLTEDTARSKPEVFARFIRSQIDLYREWQARAEAA
                     FGWIPWRSRVAVMTAGEMYKWAATKIYRNPFIVYEKKVKPSRQRIFFSLLRNASRAHR
                     CRRPEAPDQEPA"
     gene            5055..6149
                     /locus_tag="P5179_05410"
     CDS             5055..6149
                     /locus_tag="P5179_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS94689.1"
                     /translation="MMVLIACIVIAFTALQFLVALANLIFESDLPEEGEKEGPLVSVL
                     IPARNEEKNIGSIIEDLLGQDYQHIEIVVFNDQSSDRTEEVVRTYAVRDERIRLINST
                     GLPAGWTGKNYACHSLALNAKGEYFLFLDADVRISGNVIGRCLALVRKKGLDLVSVFP
                     KQEMQTPGEKITVPIINYVLVTLLPLVLVEKSRHSSLAAASGAFMFFTAGAYREVLPH
                     EKVKRAVVEDVSIARLFKKADKRVACLLGDDDYSCRMYHGFRHAVGGFSKFITTFFAD
                     SFVLSVLFFLVTTFGFLPVLWAMPSWVFAGYLVVYVLTRVIVFFLSHQNILSSVLLII
                     PVQLSLGLINCVAYLGRFSGKLEWKGRQIA"
     gene            6207..7685
                     /gene="crtI"
                     /locus_tag="P5179_05415"
     CDS             6207..7685
                     /gene="crtI"
                     /locus_tag="P5179_05415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRS94690.1"
                     /translation="MKKKVLVVGAGLGGLATALRLAKKGYEVEIVEKNSNAGGRLNQI
                     KKDGFTFDTGPSFFAMSYEFEDFARECGIALPFEHYALDPLYSVNFRKDRVTYHLHRD
                     IGKLASQFKHVEPDFENRMRRYLDKCGQVYRDTNFVIKTNYDSLPDYLSALAKANPFL
                     LPLLWRSFWKQVSRYFDSNDAREIVSLVSFFLGKTPFDTSAVYTLLSYTEFVHDGYYN
                     VVGGMYRIAEGVVRELEKENVRIHYGVEIVDFVAKDRTVEYLLDGEGRKWTADIVVVN
                     ADAAVFRGRVFRRRKFSPEMLDRMDWTMGTLTIYLGIKCKLPQLNHHNYYLGDDFTEY
                     AHRVFNDPSAAEKPYYYVNVLSRTNPDCAPEGCESLFFVCPVPDLRYKSNWDDRDAIV
                     DGIIDDFSNRTGRNIRNEIVSRTVYTPIEWRDRFNLHRGSALGLAHNMMQTGGFRPKN
                     FDEEFGNVFYVGASTVPGGGLPMVVIGSKLVTERVERFCGNP"
     gene            complement(7774..8013)
                     /locus_tag="P5179_05420"
     CDS             complement(7774..8013)
                     /locus_tag="P5179_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRS94691.1"
                     /translation="MADSVVFDRKPAVKELEPGSYAWCRCGRSKSQPFCDGSHKGTGI
                     QPVVFKLEEKKKAALCLCKQTKTPPFCDGTHRTVT"
     gene            complement(8088..8996)
                     /locus_tag="P5179_05425"
     CDS             complement(8088..8996)
                     /locus_tag="P5179_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825803.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRS94692.1"
                     /translation="MERYRSFSRVLKEQFGGPVRKISLDAGFSCPHRKGSEGGCTYCD
                     AWGSGTGASRQGRSISDQIAAAISREAAARKPAGAFLAYFQAHTNTFAPVPTLRSRFE
                     EALTDPRVVGLSIGTRPDCLPEPVLDLLDELNRRTCLWVELGLQSAHDETLRRVNRGH
                     TVAQFADAVERCHARGIRVCAHVIFGLPGETREMMRDTARFLASLGIDGVKFHSLYLV
                     PATQMEREYRSVPFPLLSQQEYAEIVADALERLPPEAVIQRLTGDPPRTIPAEPPWTR
                     DKVGTLALIRRELARRDSFQGALYRG"
     gene            9038..>10350
                     /locus_tag="P5179_05430"
     CDS             9038..>10350
                     /locus_tag="P5179_05430"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRS94693.1"
                     /translation="MQKYTLETCRKRTSGVGARTTVMALKGLRMAELRSQWGSKVGFV
                     LAASGSAIGLGNIVFFSANAYKYGGGAFYIPYIVAFCILGIPIMLMELALGAHTGKAF
                     PQSLYRIGKRPAEFIGWWGVLNATVICMYYVAILAWVLGMFIGSMKGLWSSEPVTSGA
                     FGSLALPNPFGYFFGMLGSWDTLWYVAIVWALNLLIVLRGARSIESATRFLVPLMWLC
                     MIAPIARGVSLPNGEQGAYLLFSPNFTVMADPEVWKGAFSQMFFTLSLGFGIMTAYAS
                     YLPKKSDQVHNAATICFLNCSFEFIAGLAVFSLLFAFSVVPKASTLSMMFFVVPQGIA
                     KLPHFVVGFGMLFFFLLFIAGLTSSISLVEAFVASILDKFRWKRKKAVLVVAVCSMLG
                     SMCFALPHVVDPTLASNGTLGLTLLDLFDHWSFSYGLLIVGFFECV"
BASE COUNT         2047 a         2919 c         2943 g         2341 t
ORIGIN      
        1 ggccgccaga actcctcctg tgcgggcaac gcgtctgttc gctcgtcccg ctcttcaacc
       61 tccggcggca tgacgaattc gtctttcacc gggcccgtag gcgcggcatc actcttcgtt
      121 tccccgtcca tctgtttctt ctcctcattg cagccttttt caacgaacaa ataatacgca
      181 ttcctccgcc tcccaaacca acccatttcc gtgtgtttcg tgccgcctac ggagcccgga
      241 tgagcggtcc ctgtgccgcc ttcgcccaac aactacgtct ccgtaccgcc ccctcttcgt
      301 gtccttcgcg gcttcgcgtg cggcatttcc accccgtctg tgatggtgca tcgccgcgtg
      361 ccggcgacgg gaaaacgcgg tccctgtctc ccgccgtgta cgcaaccggc cgtccttctg
      421 agcgcctcgt ggagcttcag ggaacccgcc cggtttgcgc gggcgcacat tcctgcgtat
      481 tctttcgccg cgttcaatct ttcatacgca tgacgcgtcc actcgcgaaa ggtttttcgt
      541 acggcagccg cgagtacccg tgcacaagtc gctttgctga ctgcatgcgc atgcatacgc
      601 cgacgatttc cattccagtg gtcgcgccac tgcgctgcgt ccgtcaacag acgggttgcc
      661 gccggcaggt tgccggggcg gcccgccgca cacgaaggga gaacggtatg cttggaattg
      721 cggacccgca gatctggttg gcctacgttc tgtgcatcct cagcacgatc ctgtgcgtct
      781 actacggcgc aaaaaactgg gataagggcg acgaacctgt gtaccccgag gacgtcacct
      841 gggcggagga cgagaaaaag gcaaccgcgg agaactgact ccgtgttgac accacgtggt
      901 tcacgattga acgcagacga tcgaaacacc attgattcct cgggggatac cagatggaca
      961 atttcgcaat ccttaccgtc atcgtagtcg tctacctgtt tgttgtcgcg tatctgggat
     1021 attacggcta ccggaagacg aaaaacgcaa cagattacct tgttgcggga aggagcacac
     1081 acccgtacat catggcgctg tcgtacggcg ccaccttcat cagtacgtcc gcgattgtgg
     1141 gattcggcgg ggcggctgcg ttgttcggaa tggggctgtt gtggctcacg ttcctgaaca
     1201 tctttttcgg catcttcatc gcctttgtgg tgttcgggaa gagaacccgg aaaatcgggc
     1261 gcaatctcga cgcccacacg tttcccgaat tcctggggcg ccggtttgat tcccggttca
     1321 tccaggggtt cgccggcatc accattttcc tgctcatgcc gttgtacgcc tccgcggtgc
     1381 tgatcggggc cgcgcgcttc atcgaaaaca cgttcgcggg ggtcaactac aacacggcca
     1441 tcctgatcta cacgctcatc atctgcgcgt acgtgctggc gggcggcctg aaaggcgtca
     1501 tgtataccga cgccctgcag ggcacgatca tgttcgtcgg gatgctggtg cttgcggtct
     1561 acacgtactc cacgctgggc ggagtgatag actcccacca ggcgcttacc aacatggcgg
     1621 acaaggtacc cgcgaaactg gccgcgatcg ggcatcaggg gtggaccagc atgcctgcgc
     1681 tcggttcgcc gctctggtgg cagcttgtct cgacgatcgt gctgggagtt ggcatcggcg
     1741 tgctggcgca gccgcaactc gtcgtgcgct tcatgacggt gaagagcagc cgcgagctca
     1801 accgcgcggt attgagcggc ggaatcttca tcctggcgat gacgggagtt gccttcgtgg
     1861 ttggcgctct ctccaacgtg ttcttctacg agcacaccgc gtacaaggcc atctcactgg
     1921 cggttgccaa ggggaacatt gattccatca ttcccctgta cgtcaacagc gcgatgccgg
     1981 tctggttcgt gtacctgttc atgattactc tgctttccgc ggccatgtcc acctccagtt
     2041 cgcagttcca caccatggga tcggcgttcg ggcgggattt cttcgaaaag accctgttcc
     2101 gtgggaaaca gttcctgtac accgtcagcc tgacgcgtat cgggattgtc ctcggcgtga
     2161 ttgccaccgt gattctcggc tacaaccttc ccgggagtgt tatcgccatt gcgaccgcga
     2221 tattcttcgg gttttgcgcc accatgttcc ttccgagctt cgtgggcgcg ctgttctgga
     2281 aaggcatgac gcgggcgggg gcggtttcaa gcgtggtggt agggttcttc ggttccgcgt
     2341 gctggctcct gttcgtgcat cagaaggaat cagaggcgct cgggctctgc aaactcgtgt
     2401 tcggcaaggc aagcctcgcc gccggaaccg tctgggcgat ggtggacccg atcgtggtga
     2461 ttctgccgat ttccttcgtc gttgcgttcg gtgtaagcat gtggacgaag aagatgccgc
     2521 aggagcacct cgacgcatgc ttcaaacaca tgtagcatgt ggccgcaaga ggaaaggaaa
     2581 gagggacgca gattgcgctg cgtccctctt ttttttactg cggatactca cactgcgtta
     2641 ccggtctggc ttcgtctcgt tcccccggag ggagaacctg cctcactgtt ttgaccgtga
     2701 aacaacgtct cttccgcccg cgaccctagg taatccaggg agacgaatct ccctcagcac
     2761 gcactcaacc ccagtgacac tctcagaact gcaaaaaaaa caagctgcgc gaacacgatg
     2821 atgaagggag caggattcct ccaggtaacc ctgaggtgca catatgccgc cgacatgagg
     2881 agtgacacga agaaccatgc tgcgtagttc tggagaggga tgcagttctg ctcccatttc
     2941 cagtagccca acgcgatggc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnncgcgcgt tcagggagat cggcaggcat ttcaacggtc ccttgttcgt atgaaatcaa
     3121 cgaggcttct gagacgttcc tttgagtctt ccggagccac gagagcgtct atcatctttc
     3181 ccgtttcggc ggcgagcgcg ttcattctgg aaatcaactc agatcgggcg ccgcttgttt
     3241 ctataagccg gcgggcgcgc aggatctcat ccgcggtggc gtcgcgtcgt ccgagaatgc
     3301 cggtcagttc tgagcgtgag acagggtcaa gccgcttgag ggcgatcgcg atgagaaggg
     3361 tcttttttgc cagcctgatg tcagagccta cgggcttccc gatcgcattc tccgagccga
     3421 agatgccaag ctcatcatcc ctcatctgaa acatgattcc cattgattcg ccaaggcgcc
     3481 agagcgctga gacgagttcc tcgtccgcat ttcctgccat tgctccggcc gaaagtggca
     3541 aagagaagga ataccgcgct gttttccaca ggtagaggtc aattatctcc ttttcggagg
     3601 ggatgcggga gaaggtcccg taatcgatat ccgccatctg cgcaagaccg atcgcgtaag
     3661 attcgcggca tacgaggtcc ttgacgccgt ccgggatatc cgccgatgag atcaaccgca
     3721 gcgcgatgaa cagggcgcag tcgccggcgc agatacccat ggaggtgccg taatgctctg
     3781 attcttcaat gccctttgcg tgggcctctt cgcggaactg ccagaacacc gacggcatgc
     3841 ctctcctcat gcggtcctgg tccatgatgt cgtcgtgaat caggaatgcc gaatgcagca
     3901 gttcaattgc ggcggcgatt ttgagcgttg tttccccggg ccggcccccg gccagttcat
     3961 ggcccagaaa gacaagacct ccccttatga acttcccgcc gcgtgcaaac gccccaatgc
     4021 gcgcgggcag atcctttccc caccgattca ggaaggagaa ttcctcgaca caggtctcaa
     4081 ggtgcgatcg gagaaacgct ttgatctctt cagaacggga tccgaaatat cctgcgttca
     4141 tgcgggttcc tgatctggag cttcgggtct tctgcagcga tgagctcttg acgcgttccg
     4201 cagcagcgaa aaaaagatcc tctgtcggga aggtttgact ttcttttcgt acacaatgaa
     4261 ggggttgcgg tagatctttg ttgccgccca cttgtacatc tcgccggccg tcataacggc
     4321 tacgcgagac cgccacggga tccagccgaa cgcggcttcg gcgcgtgcct gccattctcg
     4381 atacaggtct atctggctgc ggatgaagcg agcaaacacc tcgggcttcg atcgggcggt
     4441 atcttccgtc aggtcttcga ggcccgaccc ggcaaggggc agatatgttc tgcccagttc
     4501 gttgtcttcc gcgatatcgc ggatgaagtt tatgtactgc atggccctgc cgaggaaggc
     4561 tgcggcttcc atcgcgttcg gggggagttc cataatggat gccatgaaaa acccgataac
     4621 ctcggcggaa ccgtgaatgt agacaagcac ctcgtcggga gtttcgtatt tctttctggt
     4681 gagatccgcc tccatggagt tcaggaaggc ttcggcccac gcgtcgtcaa acttccttct
     4741 ccgggagagc tcgatgaagg agtctgctat cgggtccccg ctgtctctgc cagttctcct
     4801 ggcaagttca tagcgcgcgc ggaacgcgtg aaatccttct ttgtcctggg ggattgaatc
     4861 aacgaagtcg tccgctgttc gcacgaaggc atacagaatg actacgtccc tccggacttt
     4921 ctccggaaag aagagggaag agttgaagaa ggttctggac cctcttctga agatttcctc
     4981 aaatgccgat tgcgtacgtt tcatagcgac ttcaacgtgc cgaaccgcag gtcttccgag
     5041 gaactgagaa cggaatgatg gtcctgatag cctgtatcgt gatagccttc accgcgctcc
     5101 agtttctcgt ggcgctggct aatctcattt tcgaatctga tcttcctgaa gagggggaaa
     5161 aagaaggtcc ccttgtgtcc gtccttatcc ctgcaagaaa tgaagaaaag aatataggca
     5221 gtattatcga agacctcctc ggtcaggatt accagcacat tgagatagtg gttttcaacg
     5281 atcagtcttc cgacagaacg gaagaggttg tcagaacata cgcagttcgt gatgaaagaa
     5341 taaggctgat caattccacg gggcttcctg cgggatggac cgggaagaac tacgcatgcc
     5401 actctctcgc gctgaacgcg aagggggaat actttctgtt tcttgacgcg gatgtgagga
     5461 tatccggcaa cgtgataggc aggtgtcttg ctcttgtgcg gaagaaaggg ctggacctcg
     5521 tctcggtgtt tcccaaacag gaaatgcaga cgccgggcga aaaaatcacg gtgccgataa
     5581 tcaactatgt gctggttact ctcctgccgc tcgtactggt cgaaaaaagc cgccacagct
     5641 cgcttgcggc ggcaagcggc gcgttcatgt ttttcacggc aggggcttac cgggaggtac
     5701 tccctcacga aaaggtcaaa cgcgcggttg tagaggacgt aagcatagcc cgcctgttca
     5761 aaaaggcgga caagcgcgta gcctgcctgc tcggcgacga tgactattcg tgcaggatgt
     5821 accatggttt ccgccatgcg gtaggcggtt tttcaaaatt cattaccacg tttttcgcag
     5881 attcgttcgt tctctccgtg ttgtttttcc tcgttacgac gttcggtttt ctccccgtcc
     5941 tgtgggcaat gccgtcctgg gtcttcgcag ggtatctggt tgtgtatgtt ctgacgaggg
     6001 tcattgtgtt cttcctcagc catcagaaca tcctcagcag cgtgctgttg atcattccgg
     6061 tgcagctctc tctcggcttg ataaactgcg tggcgtacct cggcagattt tccggaaaac
     6121 tcgaatggaa ggggcggcag atagcctgag atagacagcc gttcgcggtg gttctgcgtt
     6181 ggtgattaat ggatgagagg cgggacgtga aaaagaaggt tctggtggtc ggagcggggc
     6241 tcggaggcct ggcaacagcg cttcggctcg cgaaaaaagg ctacgaggtt gagatcgtcg
     6301 aaaagaacag caatgccggc ggcaggctga accagatcaa gaaggatggg ttcacgttcg
     6361 ataccggtcc aagttttttc gcgatgtcct acgagtttga ggatttcgcg agagaatgcg
     6421 gcatcgcact tccctttgag cactacgcgc ttgacccgct gtactcggtc aatttcagaa
     6481 aggaccgcgt tacctatcat cttcaccggg atatcggcaa attggcgtcc cagttcaaac
     6541 atgtggaacc ggatttcgag aacaggatga ggaggtatct cgacaaatgc gggcaggtct
     6601 accgggacac gaacttcgtg atcaagacaa attacgattc tctgcctgac tacctctccg
     6661 cacttgcaaa agccaatcct tttctgctgc ccctgctctg gaggagtttc tggaaacagg
     6721 tgtcccggta cttcgactca aacgacgcgc gagagattgt atcgctcgtg tctttcttcc
     6781 ttggcaaaac gccgttcgac acctccgccg tgtacacgct tctttcttac accgagtttg
     6841 tgcatgacgg gtactacaac gtggtcggcg ggatgtacag aattgcggaa ggcgtcgttc
     6901 gcgagctcga aaaagaaaac gtcagaatac attacggcgt tgagatcgtg gactttgttg
     6961 cgaaggacag gacggttgag tatctgctgg acggagaagg caggaaatgg acggcggata
     7021 ttgtcgtcgt aaatgccgat gctgcggtat ttcgaggccg cgtgttcagg agaaggaagt
     7081 tctccccgga gatgcttgat cgcatggact ggaccatggg gacgctcacg atctatctgg
     7141 gcatcaaatg caaattgccg caactgaacc accacaatta ctacctcggt gacgatttca
     7201 ccgagtacgc acacagggtg ttcaacgatc cgagtgcggc ggaaaaaccc tattactatg
     7261 tgaacgtgct atccagaacg aatcccgact gcgctcccga gggctgtgaa agcctgttct
     7321 tcgtctgccc cgtgccggat ttgaggtaca agagcaactg ggacgacagg gacgcaattg
     7381 tggacgggat catcgacgat ttttcaaaca ggacagggag aaacatcagg aacgagatcg
     7441 tatccaggac tgtctacacg ccgatcgaat ggcgcgacag gttcaatctc cacagaggga
     7501 gcgcgctggg tcttgcgcac aacatgatgc aaacgggggg attcaggccc aaaaacttcg
     7561 atgaggagtt cgggaacgtc ttctacgtgg gagcgtcaac agtcccgggt ggcggacttc
     7621 caatggtggt aatcggctcg aaactcgtaa ccgagagagt tgagcgtttt tgcggaaacc
     7681 cttagggcga ctgataccgc ggtactccaa aaactacttg agggacgcgg ggcaggccgc
     7741 gtcccttttt tgtactcatt cgggaaagtt gcgctacgtt accgttctgt gcgtgccatc
     7801 gcagaacggg ggcgtttttg tctgcttgca caggcagaga gccgccttct tcttctcttc
     7861 cagcttgaaa accaccggtt gtatccccgt tcccttgtgc gatccatcgc agaacggctg
     7921 gcttttcgac cgaccgcacc tgcaccacgc gtacgagccg ggttccagtt ccttcaccgc
     7981 gggttttctg tcaaacacca ctgagtcagc catgccggtt cctccgaaaa cacgtgatac
     8041 gcctgaatag aaactcgtgg aaggtgagtc actgaaaata tagggtctta cccccggtaa
     8101 agcgccccct gaaaactgtc ccgtctcgcc agttctctgc ggatcagggc aagcgttccg
     8161 actttgtccc gcgtccacgg tggctccgcc ggaatggtgc ggggaggatc cccggtgagc
     8221 ctctggataa cggcctccgg cggcagccgc tcaagggcat ctgcaacgat ctccgcgtac
     8281 tcctgttgcg acagaagcgg gaacggaacg ctccggtact ccctctccat ctgcgttgcc
     8341 gggacgaggt agagcgaatg gaatttcacc ccgtcaatac ccagggatgc gaggaacctg
     8401 gcggtatcgc gcatcatttc ccgcgtttcc cccggcaacc cgaaaatcac atgagcgcac
     8461 acgcgaatgc cgcgcgcatg gcatcgttcc acggcgtcgg caaactgcgc caccgtgtga
     8521 ccgcggttga ccctccgcaa cgtttcgtcg tgcgcggact gaagcccgag ctccacccag
     8581 aggcaggtgc ggcgattcag ttcgtccaga agatccagaa ccggctccgg caggcaatcg
     8641 gggcgcgttc cgattgacaa cccgaccacg cgcgggtcgg tcagcgcttc ttcgaaccgg
     8701 ctgcgcaggg ttggaactgg cgcgaaggta ttggtgtgcg cctggaaata tgcgagaaac
     8761 gcgcctgcag gcttccgcgc cgcggcctcg cgcgaaatcg cggcggcaat ctggtcggag
     8821 atggagcgac cctgtctgga tgcgccggtg ccggaacccc acgcatcgca gtaggtgcat
     8881 cctccctcgc ttccctttcg atgcgggcag gaaaaacccg cgtcgaggga gatcttccgc
     8941 accgggcctc cgaactgctc cttcagcacg cgcgaaaagc tgcggtatcg ttccatcgtt
     9001 cctgcaacgg aaaagggcaa ggattgggac gaaaatcgtg caaaagtata ctcttgaaac
     9061 gtgccggaaa agaacgtccg gtgtcggcgc acgcaccacc gtcatggctc tgaaaggtct
     9121 gcgtatggcg gaactacgtt cacagtgggg ttccaaggtc gggtttgttc tcgcggcatc
     9181 tggttcggcc ataggcctcg ggaacatcgt cttcttcagc gccaatgcgt acaagtacgg
     9241 cgggggagcc ttttacatcc cgtacatcgt ggcgttctgc atcctcggca ttcccatcat
     9301 gctcatggag cttgctctgg gagcgcatac cggcaaggcg tttcctcagt ccctctacag
     9361 gatagggaag cgcccggcgg agttcatcgg atggtggggc gttctgaacg ccaccgtcat
     9421 ctgcatgtac tacgtcgcca tcctggcctg ggttctcgga atgttcatcg gcagcatgaa
     9481 agggttgtgg agttcggagc ccgtcacgag tggcgccttc gggtctctcg ccctccccaa
     9541 tccgttcggg tatttcttcg ggatgctcgg ttcgtgggat acgctgtggt acgtggcgat
     9601 tgtgtgggcc ctcaatctcc tgatcgttct gcgcggtgcg cggagcattg aatccgccac
     9661 ccgttttctc gtgccgctca tgtggctgtg catgatcgcc ccgatcgccc gcggcgtgag
     9721 cctgccgaac ggggaacagg gcgcatactt gctgttcagc ccgaacttca ccgtcatggc
     9781 cgacccggag gtgtggaaag gggctttcag tcagatgttc ttcaccctgt cgctcgggtt
     9841 cggcatcatg acggcgtatg cgagctatct gccgaaaaag agcgatcagg tgcacaacgc
     9901 ggcaaccatc tgctttctca actgctcatt tgagttcatt gcagggctgg ccgtgttctc
     9961 ccttcttttc gcgttctctg tggtgccgaa agccagcact ctcagcatga tgttcttcgt
    10021 ggtgccccaa gggatcgcaa aattgccgca ttttgtcgtg ggtttcggga tgctgttctt
    10081 cttcctcctg ttcatcgccg gcctcacctc aagcatttcc ctcgtggaag cctttgtcgc
    10141 gtccattctc gacaaattcc gatggaagag aaaaaaggcc gtactggtcg ttgcggtgtg
    10201 cagcatgctg ggcagcatgt gctttgctct tcctcacgtg gtagacccca cccttgccag
    10261 caacggcact ctcggattga cgctcctcga tctgttcgac cactggtcgt tcagttacgg
    10321 cctcttgatc gtcgggtttt tcgagtgcgt
//
LOCUS       DAOTNG010000080        10323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6048_length_10323_cov_5.447507, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000080 DAOTNG010000000
VERSION     DAOTNG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6048_length_10323_cov_5.447507"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5179_05435"
     CDS             complement(<1..194)
                     /locus_tag="P5179_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94694.1"
                     /translation="MVRKIVLCAAVVATVFFVGCGGEEKECVPVTVEAQTVEIITEDS
                     VTLVGTWFGVPNSRASIVCLP"
     gene            complement(323..3007)
                     /gene="aceE"
                     /locus_tag="P5179_05440"
     CDS             complement(323..3007)
                     /gene="aceE"
                     /locus_tag="P5179_05440"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRS94695.1"
                     /translation="MSETTRSGESAGMNLAEKREWLDSLYYVLRSGGPERVEGLLQML
                     QNEARVAGVDVPFSASTPHINTIPPEKQPPYPGSREIERRIKSLVRWNAMAMVVRANR
                     ASSSIGGHISTYASAATLYEVGFNHFFRGRGNGYDGDQIYFQGHASPGIYARAFLEGR
                     LTEEDLESFRQELRPGGGLSSYPHPWLMPEFWEFPTVSMGLGPIMSIYQARFNRYLED
                     RGLKKDTGGKVWAFLGDGETDEPESLGAITLAAREKLDNLIWVINCNLQRLDGPVRGN
                     GHIIEELEAAFRGAGWNVIKVVWGSEWDELIAADRDGILVRRMGEVVDGEYQKYSVEG
                     GAYIRKHFFGADPRLLKMVENYSDEHLQKMRLGGHDPEKVYAAFKAAVEHKGHPTVVL
                     ARTIKGYGLGEAGEGKNVAHQQKKMNEKELSEFRSRFGIPISDEDLATAPFYRPPDGS
                     REIEYMLERRRALGGFAPSRKPRNAPLPPPDEDLFAEFAKGTEDRGVSTTMVFVRLLT
                     KLLRDPEWGRLIVPIVPDEARTFGMEALFRQSGIYSHVGQLYDPVDRDNLLYYKEARD
                     GQILEEGITEAGSMASFIAAGTAYATHGVNTIPFFIFYSMFGLQRVGDLVWAAGDIRA
                     RGFLIGGTAGRTTLAGEGLQHQDGNSHLLAYPVPNLIAYDPAFAFELAVIIRDGMRRM
                     YQEQESVFYYVTVENENYPMPAMPAHVDPAAILKGMYKFRPASLPASEAKANLFGSGT
                     ILNEALKAQHLLEDVYNVPTDVWSITSYKELYRDGLGAERWNILHPFDPPKEPYVSSV
                     LKQETGAFVMASDYVKALPASIDRWFPTPPVCLGTDGFGRSDGRRALRRFFEVNAEHI
                     TLSALTELTRQGKFAPDDLKKAMRDLLVNPDKPNPATS"
     gene            3269..4306
                     /locus_tag="P5179_05445"
     CDS             3269..4306
                     /locus_tag="P5179_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94696.1"
                     /translation="MLTLNRLVFFLTFVSASVSSGATRYAGDFLAVGVSPRATGMGSA
                     VTAIVGGPEASYWNPAGLAAGITGAPVSVAMEHAERFGGVVKHDAVSAAIPLSRSVLG
                     VFLFRGAVDGIIFADSTTLVDPSLPLSESNMPDPSKTRTFSNADYVIHVATGHTVLRT
                     LRVGAGVKLIRRTIGGTAAFGYGVDLGAQWEPRPQIAVGILVRDATTTRVNWDNSRTD
                     VVYPTVHTGAAFGFPFPRFGGRLVVAGNSTVGSESGGYGGFAPWRVLNARNVANLGAE
                     YEWRETIALRLGSQNVRGMLGPGSAEVTAGMGLRGHLPWFSRVRRVGVDLAWIRHTLR
                     DSFRLGATVEW"
     gene            4330..5538
                     /locus_tag="P5179_05450"
     CDS             4330..5538
                     /locus_tag="P5179_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94697.1"
                     /translation="MKHVVSLVSTAGLCGVVACSSISSPTVYVRDPSYDFRGKTVGVC
                     VILDPVEQEALSDQRVVNRATSSGQTEGYASEMTEQPLEEDTGLLTPPKTVTSLQTKL
                     KHWRYYDEDFTLAMVDEVSAELQKRGFKVRVIPVPEGQVELVELAQRAQSDTCQILAV
                     VTVTVARSWNIRHQQTLRSTQDLSSSMRWVSYTGGLVLCNIAMFDIATNKVVWQHSQR
                     VVPLSRTVPVIAELYDNEVASTRFSSRPGLYQGWMYRQAMPRAVRLMFETAESGFIPL
                     PRGSESVIADTAPAPFASGCRVFVRPDSSSRLWFPATVIRDSAGVVSVQWADGIWEAY
                     GRRVEYPRSDVRPRDEFPEIVWVRTRDRLEFTPYRFVRVDVRGLVHVTRAGDMSPSTF
                     LPGRIGVVLE"
     gene            complement(5620..7716)
                     /gene="fusA"
                     /locus_tag="P5179_05455"
     CDS             complement(5620..7716)
                     /gene="fusA"
                     /locus_tag="P5179_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRS94698.1"
                     /translation="MNEHSANTIRNIAFVGHGGAGKTTFVEAVLYTLGVTTRMGSVTD
                     GNTVSDYNADEQLRKFSLNATVLQFEAGAIKMNVIDTPGYTDFIGEAVGALRAVDNVA
                     VFINAQTGVEVGTELIWGYAAEFHKPALIVINACSREHADFQQAVDQAKDRFGSSVVV
                     CQFPVNQGEHFNQIVDVVTGKLYTYEPGGKGVAKISELSGELASRAQQMREALMEAVA
                     ESDEALLETYLEAGELEEDAFIAGLKTAIKSRMVYPVLASDAHHNVGTDRILEWIAEY
                     LPSPADMGPVEATVAGSDGKVQIQCSEADPLCAYTFKTVSEAHVGELSFVRVYGGKIT
                     QNIEVANVSQNASERIGQSFFMLGKDRTDAGRINAGDIGALVKLRTTHTGDTLCDKSR
                     QVLLPGVEWPKPNISFAVVPRTKGDEDKVANGLHRLHAEDPTFYHEVDGELNQTLIYG
                     QGELHLDVMVKRLAERFGVVVDLEKPKIAYRETIRKKAEAQYRHKKQTGGRGQFGDVH
                     LRIEPLERGAGYEFVDAIVGGVIPGKFIPAVEKGVLEAMESGIIAGCKVVDVRSTVFF
                     GSYHPVDSSEIAFKLAALNAFKNAMREAGPVLLEPIYKIEVTVPEEFMGDVMGDLSGR
                     RGKILGMDAKGPFQVIRALVPLANLFRYSTDLRSMTGGRGLHTREFDHYEEVPPDIMV
                     KIQEEFQKQKEAEEKD"
     gene            8075..9313
                     /locus_tag="P5179_05460"
     CDS             8075..9313
                     /locus_tag="P5179_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRS94699.1"
                     /translation="MKFTSYQKKTLKALVLWSVIAAAAYAIAPRVPRLLRLASGMNLL
                     ELIGLLALILAIGEALGLLTQVILVGKRKPPVEGAMVGRIYRLVAVLAVLLGIAYGFG
                     KLGTIGAFFAMFGGMLLGWSLQAPVSGIAAWVLVSLKRPFRPGDRIQFPNLGLVGDVH
                     DTGIMYTMLNQVGGTVGSEEAVGRYILVPNAVLFSQVVINYTIVQESPYMLDEVVVRI
                     TYNSNWDRAEEILLKTAEEVTGDVIAATGVKPYIRSDLYDYGVNLRLRYQTRVKDRAE
                     TAYLLNKNMFKELQKCPDVDLAMPYVYSNRAGAKLVEITTEKGAVKEIPIDEITPSAR
                     ELDPHDVEQVAQSIALQGLLQPIVVTRNNATGRYDIQAGDLRLAACKQLGWKTITALV
                     QDAKEPKPVVPPISPLNPKA"
     gene            9381..10310
                     /locus_tag="P5179_05465"
     CDS             9381..10310
                     /locus_tag="P5179_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRS94700.1"
                     /translation="MIPHRTLAALALAAFFLGGCSRAERTASGGAAPRTYVDALGDTL
                     RVTDPPRRIISLAPSLTELVFALGAGSRLAGVTDFCDYPPAARTLPRVAGFNVVNLEA
                     LVAARADLVLANRGNSPTDLAAIRELGVPVFAFQIDSLPELARAARTLGGILGVQAEA
                     ESLATSWERRIASVQSVGDSVPENARPLVFFGGIAEPIYTVSRGSFIHDVIQRAGGRN
                     VFADLPTAWPRIDLETLVRRNPDIILVGYHAATERDIASLREAPGWRTIKAVREGKVY
                     VLGDAIMREGPRLVDALEEVSRVLYPRGKVLSQ"
BASE COUNT         1952 a         3177 c         3075 g         2119 t
ORIGIN      
        1 ggcaggcaga cgatgcttgc gcgcgagttc ggaacaccga accacgttcc cacgagtgtc
       61 acactatcct cggtaattat ctccaccgtc tgcgcttcga cagtgacggg cacgcattcc
      121 ttttcctcac cgccacaacc cacaaaaaaa actgtcgcta cgaccgcggc gcacagaacg
      181 attttccgta ccatcgcatc cacctccccg cgtgaaccgc cgttgcatac cctgtcccaa
      241 ggcttctgca aggacgctag taacataggg tttgaattgc gaaaaaccaa accttggtac
      301 acaaaacccg tcgcgaactc tcctacgaag tggccggatt cggtttgtcc gggttcacaa
      361 ggaggtcgcg catcgccttt ttcaggtcat ccggtgcgaa tttgccctgc cgggtgagct
      421 ccgtcagcgc cgagagcgtg atatgttccg cgttgacctc aaagaaacgt ctcaacgcgc
      481 gccggccgtc gctcctgccg aatccgtcgg tgcccaggca gaccggcggc gtggggaacc
      541 agcgatcgat cgaggcaggc agcgctttca cgtaatccga agccatcacg aacgcgcctg
      601 tttcctgttt cagcaccgac gaaacgtagg gttccttcgg cgggtcgaac ggatgaagga
      661 tgttccaccg ctccgcaccc aggccgtcgc ggtagagctc cttgtagctc gtgatgctcc
      721 acacatcggt aggcacgttg tacacgtcct ccagcaggtg ctgggctttc agagcttcgt
      781 tcagaatcgt ccccgaaccg aacagattcg ccttggcttc tgaggccggc agcgacgccg
      841 ggcggaactt gtacatgcct ttcagaatcg cagcgggatc cacatgcgca ggcatggcag
      901 gcatcgggta gttctcgttt tccacggtga cgtagtagaa caccgactcc tgctcctggt
      961 acatacggcg catcccgtcg cggataatga cggcgagctc aaacgcgaat gccgggtcgt
     1021 acgcgatgag attgggcacg ggatacgcga ggagatggct gttgccgtcc tgatgctgga
     1081 gaccttcgcc tgccagtgtt gttcgacctg cggttcctcc gatcaagaag ccgcgggcgc
     1141 ggatgtctcc cgccgcccag acgaggtccc ccacgcgttg caggccgaac atggagtaga
     1201 aaatgaagaa cgggatggtg ttcacgccgt gcgtggcgta cgcagtgcct gccgcgatga
     1261 acgaggccat cgaacccgcc tccgtgattc cctcctcgag aatctgtccg tccctggctt
     1321 ctttgtagta aagcaggttg tccctgtcca ccggatcgta caactgcccc acgtgggagt
     1381 agatgcctga ctggcggaac agcgcttcca ttccgaaggt gcgcgcttcg tcgggtacaa
     1441 tcggcacgat gagtcggccc cattccgggt cgcgaaggag ctttgtcagc agccgtacga
     1501 acaccatcgt tgtcgagaca cctcgatctt cggtgccttt ggcaaactca gcgaaaaggt
     1561 cctcgtctgg tggcggaagc ggggcattcc gtggcttgcg tgagggcgcg aatcccccga
     1621 gagctctgcg gcgctcgagc atgtattcga tctcccggct gccgtccggg ggccggtaga
     1681 atggcgcggt cgccaggtcc tcgtccgaaa tcggaattcc gaaacggctg cggaactcag
     1741 acagctcctt ttcgttcatc tttttctgct ggtgcgctac gtttttccct tcaccggctt
     1801 cgccgaggcc gtaccccttg atcgtacggg ccagaacaac cgtcgggtgg cctttgtgct
     1861 cgaccgcagc cttgaatgcc gcatagacct tctcaggatc atggcccccg aggcgcatct
     1921 tctgcaggtg ctcgtcggag tagttctcca ccatcttcag gaggcgggga tccgcgccga
     1981 aaaagtgctt gcggatgtac gcgccacctt cgacgctgta tttctggtac tcgccgtcca
     2041 ccacctcgcc catgcggcga acgagaattc cgtcgcgatc cgccgcgatg agttcgtccc
     2101 actcgctgcc ccagaccact ttgattacgt tccagcccgc gccccggaag gcggcttcca
     2161 gttcctcgat aatgtggccg tttccgcgca cagggccgtc aagccgctgg aggttgcagt
     2221 tgatcaccca gatgagattg tcgagttttt cccgcgccgc gagcgtgatg gcaccgagtg
     2281 attccggttc atccgtttca ccgtctccca gaaatgccca caccttgccg cctgtgtcct
     2341 ttttcaggcc acggtcttcc agatagcggt tgaatcgcgc ctgatagata gacatgatgg
     2401 ggccgagacc cattgacacg gtggggaatt cccagaactc aggcatcagc cacgggtggg
     2461 gatacgaaga cagaccgccg cccgggcgta actcctgacg gaagctttcc aggtcctctt
     2521 cggtcaaccg tccctcgaga aaggcgcgcg cgtagatacc gggcgaggcg tgcccctgga
     2581 agtagatctg atcgccgtcg tagccgttac cccgtccccg gaagaagtgg ttgaacccga
     2641 cctcgtacag cgtggccgcg gatgcgtacg tggagatgtg gccgccgatg ctgctgctcg
     2701 cgcggtttgc ccgaaccacc atcgccatgg cgttccagcg caccaggctc ttgatcctgc
     2761 gctcaatttc ccggcttccc gggtacggcg gctgcttttc cggcggaatc gtgttgatgt
     2821 gcggtgtgga cgcggaaaat ggcacgtcga cgcctgcgac gcgagcctca ttctgcagca
     2881 tctgaagcag gccttcaacg cgttcaggtc ctccgctgcg caatacgtag tacagggaat
     2941 ccagccattc ccttttctcc gcgaggttca tcccagcgga ttctccagac cgggtcgttt
     3001 cgctcatcgt tggcgttcct cagaatggaa aaagcgtcag atcgttccgc gaagggccgg
     3061 gaaaaaagat gcgtggaact aatgtactcc cctcgctaca ctaaggcaaa atcgatatga
     3121 gaacgctttt tcgcagattg aacgttccca ttgaagccat cgcggcgtac ttttgtctca
     3181 tggctcgccc attctggagc gggccgatgg ccttccgcgt tccctgcgga acgcgacttc
     3241 ggcacggctg atctccccgg agcgaacttt gttaacactg aaccggctgg tcttcttcct
     3301 cacgttcgtt tccgcgtcgg tctcctcggg agccacgcgg tacgcagggg acttccttgc
     3361 cgtgggggtc agcccgcgcg ctaccggaat gggctccgcg gtgacggcga tagtgggcgg
     3421 cccggaggcg tcgtactgga atccggcggg cctcgcggcg gggattaccg gcgcacccgt
     3481 ctccgtcgcg atggaacacg cggaacggtt cggcggggtt gtgaagcacg acgccgtgag
     3541 tgctgcaatt ccgctttcgc gaagcgttct cggggtgttt cttttccggg gcgcggtgga
     3601 tggaatcatc ttcgccgact ccaccacgct ggtagaccca agcctgccgt tgagcgaatc
     3661 caatatgccg gatccgtcga aaacgcggac gttctccaac gccgactacg tgatccacgt
     3721 ggcaaccggg cacacggtgc tccgcacgct cagggtggga gcgggagtga aactgatccg
     3781 ccggacgatc gggggaacgg ccgcgttcgg gtacggcgta gacctcggtg ctcagtggga
     3841 gccgcgcccg cagatcgcgg tgggaatact tgtgcgagac gccacgacca cgcgcgtaaa
     3901 ctgggataac agccgcaccg atgttgtcta ccccacggtg cataccggcg cggcattcgg
     3961 tttcccgttc cctcgttttg gtgggcggct ggtggtcgcc ggaaactcaa ccgtcggatc
     4021 ggaatcgggg gggtacggag ggttcgctcc ctggcgcgtg ctgaatgccc ggaacgtggc
     4081 gaacctcgga gccgaatacg agtggcggga gacgattgcg ctgcggcttg gaagccagaa
     4141 cgtgcgcggg atgctgggac ccggctcggc ggaagtaacc gccggtatgg gattgcgcgg
     4201 tcacctcccg tggttttccc gtgtccggcg cgtgggagtc gatctggcgt ggatccgcca
     4261 caccctccgc gattcgttcc gtctcggcgc aacggtggaa tggtagcaaa caggaagggg
     4321 ctcacattcg tgaaacacgt cgtttcgctc gtatcaacag cggggttgtg tggcgttgtg
     4381 gcgtgcagct cgatttcgtc acccacggtg tatgtgcgcg acccatcgta cgatttccgc
     4441 ggaaaaaccg taggggtgtg cgtgattctc gacccggtgg agcaggaggc cctcagcgac
     4501 cagcgggtgg tcaaccgggc aacctcgtcg gggcagaccg aagggtatgc gagcgaaatg
     4561 acggaacagc ccctggagga agacaccggg cttctgacgc cgccgaagac ggtcacgagc
     4621 ctgcagacga agttgaaaca ctggcggtac tacgacgaag atttcaccct ggcgatggtt
     4681 gatgaggtca gcgctgaact tcaaaaacga gggttcaagg ttcgggtaat tccggtcccc
     4741 gagggtcagg ttgaactcgt tgaactcgcc caacgcgcgc agtccgatac gtgccagata
     4801 cttgcagtgg taacggttac tgtcgcgcgg agctggaata tcaggcatca gcagacactg
     4861 agaagcacgc aggatctcag ctcttcgatg aggtgggttt cttacaccgg tggactcgtt
     4921 ctctgcaaca ttgccatgtt cgacatcgcc acgaacaaag tcgtgtggca gcattctcag
     4981 cgagttgtgc cgctctcccg cacagttccg gtaatcgccg agctttacga caacgaagtg
     5041 gcttccacgc gcttcagctc gcggcccggt ctgtatcagg ggtggatgta ccgccaggcg
     5101 atgccgcgag ccgtcaggct gatgttcgaa acagcggaat ccggcttcat tccgttgccg
     5161 cgtggttccg aatcggtcat tgcagacacc gccccggctc cattcgcctc cggctgccgg
     5221 gtgttcgtca ggcctgattc ctccagccgc ctgtggttcc cggcgaccgt aattcgcgac
     5281 tcggccggcg tggtttccgt gcaatgggca gacgggatat gggaggcgta cggccgacga
     5341 gtcgaatacc cgcggtcaga cgtgcgccca cgggatgagt tcccggaaat cgtctgggtg
     5401 cgaacgcgcg acagactgga attcaccccg taccggttcg tccgcgtgga cgtgcgcgga
     5461 ctcgtgcacg tcacccgggc aggcgatatg tcgcccagca ccttcctgcc gggccggatc
     5521 ggcgtcgtgc tggagtaaga gcaccgtccc ccaaaaaagg gaaaggctgc gcgagagcac
     5581 cgcgcagcct tgtttctgtc tgaacgcgtg tcgaagcgat cagtccttct cttcagcttc
     5641 cttctgcttc tggaattctt cctggatttt caccatgata tccggcggaa cttcctcgta
     5701 gtgatcgaac tcacgggtgt gaagtccgcg tccgccggtc atggaccgca ggtcggtgct
     5761 gtagcggaaa agattcgcga gaggaaccag cgcccgaatg acctggaagg gccccttcgc
     5821 atccatcccg agaatcttcc cgcgcctgcc agaaagatcg cccatcacat cgcccatgaa
     5881 ctcctcgggg acagtcacct caattttgta gatgggttca aggagaaccg gacccgcttc
     5941 ccgcatggcg ttcttgaatg cgttcagggc ggcaagtttg aaggcgattt cggaactatc
     6001 caccggatgg taggaaccga aaaacaccgt tgacctcacg tccacgacct tgcaccccgc
     6061 gatgatcccc gattccatcg cctccagcac gcccttttcc actgcgggga taaacttccc
     6121 gggaatgact ccaccgacga tggcgtccac gaactcgtat cccgcgcctc gttccagcgg
     6181 ttctatccga agatgaacgt ctccgaactg cccgcgccca ccggtctgct tcttgtgacg
     6241 gtactgcgct tcggcctttt tgcgaatggt ttcgcggtac gcgattttcg gtttctcgag
     6301 gtctacgacg actccgaacc gctctgcgag gcgcttcacc atcacatcga ggtgaagctc
     6361 gccctgtccg tagatgaggg tctggttcaa ctcaccatcg acctcgtggt agaaggtcgg
     6421 gtcttctgcg tggagccggt gcaaaccgtt tgccaccttg tcttcatcgc ccttcgtgcg
     6481 gggaacaacg gcgaaactga tgttcggttt gggccattcc actcccggaa gaagcacctg
     6541 gcggctcttg tcgcacagcg tgtccccggt atgcgtggtc cgcagcttca caagcgcgcc
     6601 aatgtctccc gcgttgatgc ggccggcgtc cgtccggtcc ttccccagca tgaagaagct
     6661 ctggccgatt cgctccgaag cgttctgcga tacgttcgcc acctcgatat tctgcgtgat
     6721 cttgccgccg taaacccgca cgaaggaaag ctcccccacg tgtgcttcgg acacggtttt
     6781 gaaggtgtac gcgcacagag gatcggcctc tgagcattgg atctgcactt tgccgtccga
     6841 gcctgccacc gtggcttcca caggccccat atctgcgggc gagggaagat attcagcaat
     6901 ccactcgagg atacggtcgg tgccgacgtt gtggtgcgca tcggaggcaa gcacggggta
     6961 caccatgcga ctcttgatcg cggttttcaa cccggcgatg aaggcatctt cttccagctc
     7021 ccctgcttcg aggtacgtct cgaggagcgc ttcgtctgat tccgccaccg cttccatgag
     7081 cgcttccctc atttgttgcg cacgggaagc aagttcgccg gaaagctcac tgattttcgc
     7141 cacacccttg ccaccgggtt cgtacgtata cagcttcccg gtcaccacat ccacaatctg
     7201 gttgaaatgc tcgccctggt tgacggggaa ctggcacaca acaacgctgc tgccgaatcg
     7261 gtccttcgcc tgatcgaccg cctgctggaa atcagcatgt tcgcggctgc acgcgttgat
     7321 cacgatgagt gccggtttgt ggaattcggc ggcatacccc cagatgagtt ccgtgccgac
     7381 ttcgacgccc gtctgcgcat tgatgaacac cgcgacgttg tccaccgcgc gcaacgctcc
     7441 aaccgcttcc ccgatgaaat cggtgtatcc gggcgtgtca atcacgttca tcttgatcgc
     7501 cccggcctcg aactgcaaca ccgttgcgtt cagcgaaaac ttcctgagct gctcatcggc
     7561 gttgtagtcg cttaccgtgt tcccgtcggt taccgacccc atgcgggttg tcaccccgag
     7621 ggtatacagc accgcctcca cgaaggtggt tttcccggct cctccgtggc cgacaaacgc
     7681 gatgttccgt atggtgtttg cgctgtgttc gttcatgcga atgctcctgc agatttacgg
     7741 gaagatgagg cgatgcaccg gtaggcggtt cgcaaacatg aaccaataaa aatacgagtt
     7801 tgccgtacaa ggtcaaggaa gggactggcg aaaggcgatt ccatggggcc ggccacggaa
     7861 aaccggaagc ggacgatgcg ggcggatgct tcggttgccg cattactccg ccaatcccgt
     7921 gcaggagagc ccggaacgcg gacaccgaca ggacaaaatg cggaggatag ttccccgggg
     7981 cacactcctc ctctccgtcc ttatttgcgc ctttcttggt atattacatg aatctgaaac
     8041 cagaccagaa aacccgtggc ggaggtaagc cggcgtgaag ttcaccagtt accagaaaaa
     8101 gacactcaag gcgcttgtgc tgtggagtgt catcgcagcc gccgcgtacg ccatcgcacc
     8161 cagggtgcct cgtttgttgc gattggcgtc cggcatgaat ctgctggagt tgatcggcct
     8221 cctcgcgctg attcttgcga tcggcgaggc ccttggcttg ctcactcagg tcattctcgt
     8281 aggaaaacgg aagccgccgg tcgagggcgc gatggtgggc cgcatctacc ggcttgtggc
     8341 ggtgctggca gtgcttctcg gcatcgcata cggattcggc aagctgggca ccatcggtgc
     8401 gttcttcgcc atgttcggag gtatgctcct cggctggtcc ttgcaggcac cggtgagtgg
     8461 tatcgcggca tgggttctgg tatcgctgaa acgcccgttc aggcccggtg accggatcca
     8521 gttccccaat cttggacttg tgggagacgt tcacgatacc ggcatcatgt acacgatgct
     8581 gaaccaggtc ggcggaacgg tcggcagcga agaggcagtc gggcggtaca tccttgttcc
     8641 caatgccgtt ctgttcagcc aggtagtcat caactacacc atcgtccagg aaagccccta
     8701 catgctggac gaagtggtcg tccggatcac gtacaactcc aactgggacc gggcggagga
     8761 gattctgctc aagaccgcgg aagaagtcac gggggacgtg atcgctgcca cgggtgtgaa
     8821 accttacatc cgctccgacc tgtacgatta cggtgtcaac ctgaggctgc ggtatcagac
     8881 ccgtgtgaaa gatcgtgccg aaaccgcgta tctgctgaac aaaaacatgt tcaaggaact
     8941 ccagaagtgc cctgacgtgg acctcgccat gccgtacgtc tactccaacc gcgcaggcgc
     9001 caaactcgtc gaaatcacga ccgaaaaggg tgccgtgaag gaaatcccga tcgacgagat
     9061 caccccgtca gcccgtgagc tggacccgca cgacgttgaa caggtcgcgc agagcatcgc
     9121 acttcagggt ctcctgcaac cgatcgtcgt tacgcgaaac aatgcgacgg gaaggtacga
     9181 tatacaggcc ggcgatctcc gcctcgccgc gtgcaaacag cttggctgga aaacgatcac
     9241 cgctctggtg caggacgcca aggaaccgaa accggtcgtc ccgccgattt ctccactcaa
     9301 cccgaaagca tgatgaatca tccgaggggt ggcgtgcgcc gccccttccc cggttttcgc
     9361 atcccgacag gtacttcttc atgattcctc atcgaacact ggcggcactc gctctcgccg
     9421 ccttctttct cggaggctgt tcgcgcgcgg aacgcacggc gtcaggcggc gccgcccctc
     9481 gaacatacgt agatgcactc ggagacactc tccgcgtcac tgatccaccc cggcgcatca
     9541 tctctctcgc gccgagtctc acggaattgg tgtttgcgct cggtgccgga agccgattgg
     9601 cgggggtgac cgacttctgc gattacccgc ccgccgcccg gactctgccg cgtgtggccg
     9661 gtttcaacgt cgtgaatctg gaagcgcttg tcgcggcacg cgcggacctc gtgcttgcca
     9721 accgaggcaa ctccccgacc gatctcgcgg ccatccgtga acttggagtt cccgtgttcg
     9781 cattccagat agactcgctc cccgaattgg cccgggcagc gcggactctg ggaggaatcc
     9841 tcggggttca ggccgaagcg gagagcctcg ccacctcgtg ggaacggagg attgcctcgg
     9901 tgcaatcggt gggggattcc gtacccgaaa acgcgcgtcc cctcgtcttc ttcggaggca
     9961 tcgccgaacc gatctacacg gtttcccgcg ggtcattcat tcacgacgtg attcaacgcg
    10021 cgggaggccg gaacgtcttc gccgacctgc caacggcatg gccgcgcatc gatcttgaga
    10081 ccctggtccg ccggaacccc gatatcatcc ttgtgggata ccatgcggcg acagaacggg
    10141 atatcgcatc cctgcgcgaa gcgccgggct ggagaacgat caaggcggtg cgcgaaggga
    10201 aggtttatgt ccttggggac gccatcatgc gggagggacc gaggctcgtg gacgcgctgg
    10261 aggaggtctc gcgcgtgttg tacccccgcg ggaaagtctt gtcgcaataa ccaaaaaacc
    10321 ggc
//
LOCUS       DAOTNG010000081        10190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6160_length_10190_cov_4.802861, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000081 DAOTNG010000000
VERSION     DAOTNG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6160_length_10190_cov_4.802861"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="gltX"
                     /locus_tag="P5179_05470"
     CDS             <1..1252
                     /gene="gltX"
                     /locus_tag="P5179_05470"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRS94701.1"
                     /translation="GPYVQSCRLDIYRQHVDNLIASGHAYRCFCTEERLADLREAQRL
                     AKKSPGYDRRCRNLSGEEIRTALDSHTPFVVRMKIPLTGETRFEDRIRGTVEYDNKVL
                     DDFVLLKSDGYPTYHLANVVDDHLMGITHVIRGEEWIPSTPKHVLLYDAFGWADSLPE
                     FVHVPSILSPDGGKLSKRKGAAAVTDFAEMGYLPEAVRNFIALLGWSPGGDREKMALD
                     EMIEAFTLDRVNPKGAIFDESKLEWLNGEYMKDMAAVDLLPRVAPLWIEKGLIAENDV
                     ENDREYLCHVIELFKGRTRRTHDFVDLAGFCLRAPTSYDEKARAKHWSDPAAADRLAA
                     AADAFANLEPFDARTTEEVVRLLSEKEGVSASRYIHPIRLAVSGMSFGPGLFEMLEVL
                     GKGRVVERLRTAVHVIRSDRVGTD"
     gene            1338..1686
                     /gene="ssrA"
                     /locus_tag="P5179_05475"
     tmRNA           1338..1686
                     /gene="ssrA"
                     /locus_tag="P5179_05475"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1851..2693
                     /locus_tag="P5179_05480"
     CDS             1851..2693
                     /locus_tag="P5179_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRS94702.1"
                     /translation="MTAKIASIWEMHYQWILDFGYKVLLTVVVLAASYLAAKAIRRSI
                     TRANERFERLDATLVPVLTALATYAVYALGTIIVLDILGVNTASIITVLGATALAIGL
                     ALKDTLGNLAAGIMLLILRPFRAGDLIKCGTIEGTVEEARLFTTKLQTIDGLFVEAPN
                     SELWNNHITNFTRNGKRRMDIVVGISYSDSIDKGLEVLAQLVREEPRFLNEPPPQAMV
                     KALSDSCVEIQLRAWTSIDDYWNTYFDQNRRVKLAIEAAGLTIPFPQRDVHVVSGNMA
                     GPSQ"
     gene            2800..4920
                     /locus_tag="P5179_05485"
     CDS             2800..4920
                     /locus_tag="P5179_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRS94703.1"
                     /translation="MVLDNGVLHLEFDDTTGLLSHLIDVQGRVVHLAPPDEPLGIWKL
                     VFRAGTDEQSLVWPPWKGEVEMSCVREPDGTQIASIEWRDMWFRHEPKAVDVAVTVRL
                     PENSGTSEWRIRVSNRSARYGLWEVRFPWFNRWPAPKEYNVAIPWHNWGHRYQKVAER
                     HTGEYSSYTWSMQFLTFESGQRGLTIAALDPEQHYKSYLVDPGNETAFVLRVPDGSKP
                     GVGLDDTYATSISVHGDGWFGGCKLYREWALGQKWCAKGPISRRKDVPKTLKDIGIWL
                     LSSIPEKNSGVTPAEWADGVIAAQRYFDVPVGIQIYQWHEIKFDNDYPEYFPPKPGVP
                     EAVRKLVDAGIVAMPYINARLWDMRAKSYPGAVPSTAKDPTGTPYLEIYGPESGLLTP
                     MCTQTKLWQDTVFNLCRRIYEEIGANAIYLDQVAAMPAAPCYDPTHGHPLGGGHHWHD
                     GYRVILNRVKDYIASTGKDLFLTTENPGDAHIDGCDAFLIWNPRQDTEVPMMTSVYSG
                     YTLYYASNHTTGEGMQSFVMAQARDFLWGSQLGWMPPEIPEPFRGYLKTLGKLRVQTR
                     KFLTFGELIGMPQNTAPDEIRGSSDEMMRKREESIAKVSANWPFFGTPKIATLPAVMS
                     ALWRAEDGHLGLFIMNLSEVKQSYCCAFDPPAYGLTSERLRYTRIQENGPGDSFEVQG
                     GVQVRSEDLEPFGGVVYDIGPAGT"
     assembly_gap    5057..5156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5157..5374
                     /locus_tag="P5179_05490"
     CDS             <5157..5374
                     /locus_tag="P5179_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94704.1"
                     /translation="NLAGRHASFREVAIALDRIRAAHMPTDGASLRVFAAGIRHRPAR
                     DEICSTRIDIIGVIGLSTVDNVAHEKI"
     gene            5407..6588
                     /locus_tag="P5179_05495"
     CDS             5407..6588
                     /locus_tag="P5179_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HRS94705.1"
                     /translation="MPKGAVYPPEMSKYVDPVSGNEVVQLTSTRKWNVHPTYHVNGFL
                     DDQRIVFAAEAEGATNIYACSLVAGEITQLTAGRGTSYLLAYLAGERGDGRGTDAFHI
                     VAGYSSHAVYFVEGDELRTVNADTLEEESLYRIGPEWVCGVLELSGDEQTVLLPLLPR
                     ECFNTRCDLAEFLRRTDRLALPSRLVGVSVVDGRSQVLWEDAGRFIGHAAFSPVSDKL
                     VLCERATDPDRREEPLLWLLKLEEAEPIPFPTRNPKTGHSVWLPDGTGVVTHGMVIDG
                     GREHGAEYIQILNLDTSARWIGYHGPPRYYGHCHTTPDGQTIIVDGVFAPDQITAVTP
                     HGDGYFTEPICVHGTDSSGGQRAHAHPHVSPDCEWLVFGSLRGGKKDLFAVRVKGLAK
                     G"
     gene            complement(6578..7807)
                     /locus_tag="P5179_05500"
     CDS             complement(6578..7807)
                     /locus_tag="P5179_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS94706.1"
                     /translation="MGDVEGLDRASWWQNARFGMFIHFGLPTVLERGIWAQYWEHIPR
                     DEYAALKRSFNPESCDAVEWVRLARDSGMKYVVLTVRNHDGFCLFDTATTDFNSVASP
                     FGRDLTREFADACRAAGMRTGFYFSLMNWRHPGCLQRDQKQYRPPAFYERLVEETHEQ
                     IRELTSNYGKVDVLWFDCMRPDDPAIWRSEEMIAMVRRNQPSILVNDRAGVPGDFGTP
                     ENEATPQSRPWEACFTMTDTWGYAPGDRNWKTSRELVTLLMSCAARSGNLLLNLPPGA
                     DGRFPREGVGRLREIGKWLRTNGEAIYGTQSAPIGHALNVIGPGQGWATVRGSTIYMA
                     AMRWQGPTFRFAWLKNRVVSARVLGADCEVRVEQKGDRVWLHGLPEHPPDPLLPVLAI
                     TVEGTPERIEPGEFLIL"
     gene            complement(7800..9269)
                     /locus_tag="P5179_05505"
     CDS             complement(7800..9269)
                     /locus_tag="P5179_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HRS94707.1"
                     /translation="MDTSRLFDVASFGARSHAPETSTHAFQKAIDACAESGGGTVFVP
                     PGEYVIGRVLLRSNVTFHLAAGALVKPSREKRDYPPIPNLPDSNYRAELLDNALNSRY
                     AILYALNAVNVTVCGRGCIDGDDRAFWTEKNTGTYDEWNMIAQWFYYAPNAFRPVTLM
                     FERCKNIVVRDVELVRASCYSGWFAGCTRLQFENVTVKNDHAGPNTDGFHFSSCRNVT
                     ITGCDFTCGDDCIAIDPNYGGIAENYTVSGCRFDTSVNAFRIYTGLDPGLPHEMPRGE
                     VRNVVAGTCIVANASGVFNVTAENGEIHHLMFSDFCIQMAYRGSAFFFIALERGRINR
                     VTLSRMLIGTDGVGTIVSREKGAVRGIVLDGLDYVVSPRTKLYGNGAPPVLDSWGWHN
                     FAPYNLYVRGAEGVVIRDTKIVWTEGDLADLDKIPDGSPDWPAIECRDVNGLVLDNVI
                     CSAHGENHPDVLLVNVKSPEVASCRRVDGERATVEERHG"
     gene            complement(9315..>10190)
                     /locus_tag="P5179_05510"
     CDS             complement(9315..>10190)
                     /locus_tag="P5179_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94708.1"
                     /translation="ENGFEIIESESGEITRQVVENDVTYSMPEFVRYPVRDRESWEFY
                     KERTRSRRVMSPEEMEANCRRYETRDRPLCVGAGSTYGHIRGLMGTEGASVTLIEDPE
                     LAHDIIETGLKYHREFVFPLIERLRPEVVQMGEDLCFNHGMLLSPRHFREFCGEYYRE
                     VCDCAFANGAEIVAIDTDGNAMEFTGVASEYGVNAIFPYEVKAGNDLFELRQNHPEFV
                     FFGWLEKEVVNEGNEALIEPEILSKVPQLLARGRYFPNGDHGIQPPVTFRNLCRFMTL
                     LHEACGNPEGTFPRV"
BASE COUNT         2168 a         2900 c         2917 g         2105 t
ORIGIN      
        1 tgggccgtac gttcagtcgt gcaggcttga tatataccgg caacacgtcg acaaccttat
       61 cgcgagcggg catgcatacc gttgtttttg tacagaagag cgtctcgccg atctgcgtga
      121 ggcccagcga ctcgcaaaaa agtcacccgg ttatgacaga aggtgccgca acctttcggg
      181 agaagagatc cgaactgccc tcgattcgca cacgcccttc gtggtgcgga tgaaaatccc
      241 gttgaccggc gaaacacgtt ttgaagacag gatccgcggc acagtggagt acgacaacaa
      301 ggtgctggac gatttcgtcc tgctcaagag tgatgggtac ccgacatacc acctcgcgaa
      361 tgtcgtggac gatcatctca tgggtatcac tcacgtaatt cgaggcgaag agtggatccc
      421 cagcacgccc aaacatgtgc tcctttacga cgcgttcggg tgggctgact cgctgccgga
      481 attcgttcac gtaccctcca ttctgtcgcc tgacgggggg aagctttcga aacggaaggg
      541 cgctgcagcg gtcacggatt tcgcggagat gggttacctt cctgaagccg tccggaactt
      601 catcgcgttg ctggggtggt cgccgggcgg agaccgcgaa aaaatggccc ttgatgagat
      661 gatcgaggcg tttacgctgg atcgtgtaaa ccccaaaggc gcaatcttcg acgaatcgaa
      721 gcttgagtgg ttgaacggcg agtatatgaa agacatggcg gccgtggacc tgcttccacg
      781 cgttgcgcct ttgtggatag agaaaggtct gatcgcagaa aacgacgttg aaaacgaccg
      841 cgagtacctc tgccatgtga tcgaactctt caaaggccgg acgcgccgca cccacgattt
      901 tgtggatctc gccggtttct gccttcgtgc ccccacgtcg tacgatgaga aggcgcgggc
      961 gaaacactgg agtgatcctg cagcagccga tcgtctggct gctgccgcgg acgcattcgc
     1021 gaacctggaa cccttcgacg ctcggactac cgaagaagtc gttcggcttc tgagcgaaaa
     1081 ggagggtgtg agcgcatcca gatacatcca cccgatacgg ctcgccgtaa gcggcatgtc
     1141 gttcggcccg ggcctttttg agatgctcga agtgctcgga aaaggacgcg tggtcgaaag
     1201 gttgcgaacg gccgtgcacg ttatccgttc cgaccgggtg ggaactgact gattcgctgg
     1261 gcgttattcc tattggaaac aggtgttgcc aattcgggct cgatacaagc ccgatcggaa
     1321 ctcacaaaga actgaaaggg ggcgaaaagg cttcgacggg agtagtgggg ctctggtcgc
     1381 gcgccgaggt gcactgcctc gctaaacagg tgcaaaacta cgataactga caactacagt
     1441 tacgcaatgg ctgcctaaga ggtagcccgt ccccctgcat actgctcgcg cagtgcagga
     1501 aggggcgccg aacgcgcggg ctggttggtg gggtggcccg ttcccgccaa cgagattcag
     1561 ggctggcctg ccgatatgct gagtctgtcc gggaattggc gggcgagaag tgatgacaga
     1621 ctacgcgcgt agtgatcggg gtttggctgc tttcggacgt gggttcgact cccaccgcct
     1681 ccaccatttc ccctttgcaa ctgccctggc tgcgtttgac cgcaggcggg gcagttgcgc
     1741 ttttcctccc ccgtcacgtt gcgattcctt tccggttgga cctacattga cacatcgtgt
     1801 aggcgaacag caggatgtgc catcggggaa aacattccag gggatcacaa atgacggcaa
     1861 aaatcgcctc gatttgggaa atgcattatc aatggattct cgacttcggg tacaaagtgt
     1921 tgcttacggt ggtggtgctg gccgcgagct atctcgcagc aaaggccatt cggaggtcaa
     1981 taacccgggc gaacgaacga ttcgaacgac tggacgcaac ccttgttccc gttctcactg
     2041 cgctggcgac ctacgcggtg tatgcccttg gaacaatcat tgtcctcgac attctgggcg
     2101 taaacacggc gagcataatc acggtgctcg gcgccaccgc gttggcaatc ggcctcgctc
     2161 tcaaggacac gctcggcaat ctcgccgccg gcatcatgtt gctcattctg cgtccatttc
     2221 gagccgggga tttgatcaaa tgcggtacta tcgaaggcac ggtcgaggaa gcgcgtcttt
     2281 tcaccacaaa gcttcagaca atcgacggtc tgttcgtgga agcgcccaat tccgagctgt
     2341 ggaacaacca tatcaccaac ttcacgcgca acggcaaacg ccgcatggac attgtggtgg
     2401 gcatctcgta ttcggattcc atagacaagg gtctggaagt actggcgcag ctcgtccgcg
     2461 aggaaccgcg gttcctgaac gagccgcctc cacaggctat ggtgaaagcc ctttctgaca
     2521 gttgtgtgga aattcagttg cgagcttgga cgagtattga cgactactgg aacacgtact
     2581 tcgaccagaa ccggcgggta aaactggcca tcgaggccgc agggctgacc attcccttcc
     2641 cgcaacgaga tgttcatgtt gtttcgggga atatggcggg gccgtcgcag tagaattctc
     2701 gaacagaggt agcattctgt cgccgacgtg gcggcatttc gttccttttc atccgccggt
     2761 gtggagacaa cacccggcgg gccggctcgg gagggcacaa tggtcctgga taatggcgtt
     2821 ctgcacctgg aattcgacga cacaaccggt ctcctttcgc acctgatcga tgttcagggc
     2881 cgcgttgttc atctggcgcc gcccgacgag ccgctgggaa tctggaagct cgtcttccgc
     2941 gcgggaaccg acgagcaatc actcgtgtgg cctccgtgga aaggtgaggt agagatgagt
     3001 tgcgtccgag aacccgacgg aactcagatc gcatccatcg aatggcgtga catgtggttc
     3061 cgacacgagc cgaaggcggt ggacgttgcc gtgacagtac ggcttccgga aaactcgggt
     3121 acttccgaat ggcggatacg cgtttccaac cggagcgctc ggtacggcct gtgggaggtt
     3181 cgcttcccct ggttcaatag gtggccggct ccgaaggaat acaacgtggc gattccttgg
     3241 cacaactggg gacaccggta ccagaaggtc gcggaaaggc acacgggcga atactcgagc
     3301 tacacctggt cgatgcagtt tctcacgttt gaatcggggc aacgggggct gacaattgcg
     3361 gccttggacc ccgagcagca ttacaaatcg tacctggtgg acccggggaa cgaaaccgcg
     3421 ttcgttcttc gcgtgccgga cggttccaag cctggcgtcg ggctggacga cacctacgcc
     3481 acctcgatca gcgtgcacgg cgacggctgg tttggcggct gcaagttgta ccgggaatgg
     3541 gcgcttggac agaagtggtg cgcaaaaggg ccgatttcga ggcgaaagga tgttccgaaa
     3601 acgttgaaag acattggaat ctggcttctt tccagcattc ccgagaagaa cagcggagtc
     3661 acccctgcgg aatgggcgga cggggttatt gccgcgcaac gatatttcga cgtacccgta
     3721 gggattcaga tttaccagtg gcacgagatc aagttcgaca atgactaccc ggagtatttc
     3781 cccccgaaac ccggtgttcc cgaagccgtg cggaaactcg ttgatgccgg aatcgtcgcc
     3841 atgccctaca tcaacgcacg attgtgggac atgcgcgcga agtcctaccc cggggccgtc
     3901 ccttccaccg cgaaggaccc gaccgggact ccttacctcg agatttacgg cccggaaagt
     3961 ggtttgctta cacccatgtg cacacagacg aagctctggc aggacactgt gttcaatctc
     4021 tgcaggagaa tctacgaaga gatcggcgca aacgcgattt atctcgacca agtcgccgcg
     4081 atgccagcag cgccctgcta tgaccccacg cacggtcatc cgttgggcgg cggccatcac
     4141 tggcacgacg gttaccgcgt gattttgaac agagtgaaag actacattgc ctccacgggg
     4201 aaagaccttt tcctgacaac ggagaacccg ggcgacgcgc acatagatgg gtgcgacgca
     4261 tttctcatct ggaatccgcg tcaggacacg gaagtgccga tgatgacctc tgtctattcc
     4321 gggtatacgt tatactacgc aagcaaccac accaccggcg aaggcatgca gtcattcgtg
     4381 atggcgcagg cacgtgactt cctctgggga tcacagcttg ggtggatgcc tcctgaaatc
     4441 ccggagccat tccgcggata cctgaaaaca cttgggaaac tgcgtgttca gacgcgcaaa
     4501 ttcctgacgt tcggggaact aatcggaatg ccacaaaaca cagctccgga cgagatacgc
     4561 ggcagctccg acgagatgat gcgaaaacga gaggaatcca tcgcgaaggt cagtgccaat
     4621 tggccgttct tcggaacgcc gaaaattgcc acgctccctg cggtgatgtc tgcgctatgg
     4681 cgcgcggagg acggacacct gggtctgttc atcatgaatc tttcagaggt gaagcagtcg
     4741 tactgctgcg cgtttgatcc gccggcgtat ggtctcacat cggagcggtt gcgatacacg
     4801 aggattcagg aaaacgggcc tggggattcg tttgaggtac aggggggcgt tcaggtgcga
     4861 tcggaagacc tcgagccatt cggaggcgtt gtgtacgaca taggccctgc gggcacctga
     4921 caagcgggct ccaggaaatg acatggtgca aggaaaatcc gtaacggttc cgcgccgcac
     4981 tgctttcccg gcgaggttgt cacaacctcg ccgggaggca tgcaagcttc agagaagtag
     5041 cgattgctct tgatcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacaa
     5161 cctcgccggg aggcatgcaa gcttcagaga agtagcgatt gctcttgatc gcatccgagc
     5221 ggcgcacatg cccaccgacg gtgcaagttt gagggtgttt gcggcgggca taagacaccg
     5281 acccgcacgc gacgaaatct gctcaacgcg aattgacata attggtgtta tcggtctgtc
     5341 aaccgtggac aatgtggctc acgagaagat ctgagcacgg ctgcaatcgc ctggagatgg
     5401 aacgacgtgc caaagggggc tgtgtacccg cccgagatgt cgaagtacgt tgacccggtg
     5461 tcgggcaatg aggttgttca actcacgtcg acgcgtaagt ggaacgtaca ccccacctac
     5521 cacgtgaatg ggttcctgga cgaccagcga attgtgtttg ccgcggaagc cgaaggtgcg
     5581 accaacatct acgcatgttc acttgtggca ggcgagatta cccagttgac cgcgggcaga
     5641 ggcacatctt atctcctggc gtatctcgcg ggcgaacgcg gagacggccg gggaacggat
     5701 gcatttcata tcgttgcagg ttactcgagc catgccgtgt actttgtcga aggagacgaa
     5761 ctgcgcacgg tgaacgctga caccctggag gaggaatctc tttatcgaat cggtccggaa
     5821 tgggtatgtg gagtactgga actgagcggg gacgaacaga ccgtactttt gccgcttctg
     5881 ccccgcgagt gtttcaacac gcgctgcgat ctcgccgaat tccttcgcag aactgatcgt
     5941 ctcgcgttgc ccagccgcct cgtaggtgtg tcggtggtgg acgggagatc gcaagtcttg
     6001 tgggaagacg cgggacgctt cattgggcac gcggcgttct caccggtctc cgacaagttg
     6061 gttctgtgtg agcgagcaac ggacccggat cgacgcgagg aaccgttgct ttggctgttg
     6121 aaacttgagg aagcagagcc aataccattt ccaaccagga acccgaagac cgggcacagc
     6181 gtgtggcttc ccgacggcac aggtgttgtt actcacggca tggtgatcga cggaggtcgc
     6241 gagcacgggg ccgagtacat tcagattctg aacctggata cgagcgcccg ctggattggc
     6301 taccacggcc cgccgcgtta ctacggccac tgccatacta cgccggacgg ccagaccatc
     6361 atcgtagatg gagtgtttgc gccggaccag ataaccgcgg tcacgcccca cggcgacggg
     6421 tatttcaccg agccaatctg cgttcacgga accgattcat ccggggggca acgtgcgcac
     6481 gcgcatccgc atgtatcacc ggactgtgag tggctggttt tcggttcgct tcgcggtggc
     6541 aagaaggatt tgtttgccgt ccgcgtcaag ggactggcta aaggatgagg aactcacccg
     6601 gttcaatccg ctccggcgtg ccttccaccg tgattgcgag cacggggagg agcggatcag
     6661 gcggatgttc cggcaacccg tgcaaccaga cgcgatctcc tttctgttcc acgcgcacct
     6721 cgcaatcagc gcccagtacc cttgccgaca ccacgcggtt tttcagccat gcaaatctga
     6781 acgttggtcc ctgccatcgc attgcggcca tgtagatcgt ggaaccgcgc acagtcgccc
     6841 atccctgccc cggtccgata acattcaacg catgcccgat aggcgcactt tgcgtcccgt
     6901 agatcgcttc tccattcgtt cgtaaccact ttccgatctc ccgcaatcga cccacgcctt
     6961 ctcgaggaaa tcggccatct gctccgggtg gaaggttgag aagaaggttc cccgaccgcg
     7021 ccgcgcagga catgaggagc gtaaccagct ctcgtgaagt tttccagttc cggtccccgg
     7081 gagcgtaccc ccatgtatcg gtcattgtga aacacgcttc ccaaggcctg gattgggggg
     7141 tagcctcatt ctccggtgtt ccgaagtcgc ccggtacccc ggcccgatcg ttcaccagga
     7201 tggagggctg attccgacgg accatcgcta tcatttcttc cgaccgccag atcgcggggt
     7261 catctggtct catgcaatcg aaccagagga cgtctacctt gccgtaatta ctcgtcaatt
     7321 cgcggatctg ttcatgagtt tcctccacga gacgttcgta aaacgcgggt ggccggtact
     7381 gcttctgatc ccgctggaga cagccaggat ggcgccagtt cataagcgaa aagtagaagc
     7441 cggtccgcat tcctgccgcg cggcacgcgt cggcgaactc acgcgtcagg tctctaccga
     7501 aaggggatgc gacactgttg aaatctgtcg tcgccgtgtc gaaaagacag aaaccatcgt
     7561 ggttgcgaac cgtaagaacc acgtatttca tgcccgaatc tcgggccaga cgcacccatt
     7621 ccacagcgtc acacgactcc gggttgaacg accttttgag agccgcgtac tcatcccgag
     7681 gaatgtgttc ccagtactgc gcccagatgc ctcgttctaa gacggttggc aacccgaaat
     7741 ggatgaacat cccgaagcga gcgttttgcc accacgatgc tcgatccaac ccctcgacgt
     7801 cacccatgcc tttcctccac cgttgcacgc tcaccatcca cccgccgaca ggatgcgact
     7861 tcagggctct tcacattcac aagcaggaca tcggggtgat tctccccgtg ggcggaacag
     7921 atgacgttat ccagcacgag accgttcacg tcacggcatt ctatcgcggg ccagtcgggg
     7981 ctgccgtccg gaattttgtc cagatccgcg agatctcctt ccgtccagac tatcttcgta
     8041 tccctgatca ctacaccctc tgccccgcgc acatataagt tgtaaggcgc aaaattgtgc
     8101 cagccccagc tatcgagcac tggaggtgct ccgttgccgt acagcttcgt tctgggtgag
     8161 acgacatagt ccagtccgtc gagtacaatt ccccgcaccg cgcctttttc tcgtgagacg
     8221 atcgtcccga caccatccgt gccgatcagc atccgggaga gcgtgacgcg gttgatgcgc
     8281 ccccgttcca aggcgatgaa gaagaatgcc gatccgcggt acgccatctg aatgcagaaa
     8341 tccgagaaca tgagatgatg gatctcgcca ttctccgccg taacgttgaa aacccctgac
     8401 gcgttggcga cgatgcacgt gcccgcgacg acattccgta cctctcccct gggcatttca
     8461 tgtggcaacc caggatccag tccggtgtag attcgaaacg cgttcacact cgtatcgaat
     8521 cggcacccgg aaactgtgta gttctccgcg ataccaccat aattcgggtc gatggcgatg
     8581 caatcgtccc cgcaggtgaa gtcgcacccg gtgatcgtga cattgcggca cgaactgaag
     8641 tggaatccgt cggtatttgg acccgcgtgg tcgttcttca cggtcacatt ctcaaactgg
     8701 agtcgcgtgc accccgcaaa ccagcccgaa taacaggatg ccctgaccaa ctccacatcg
     8761 cgaaccacaa tgttcttgca tcgctcgaac atcagcgtaa caggccggaa tgcgttggga
     8821 gcgtaataga accactgcgc gatcatgttc cattcgtcgt acgtgcctgt gtttttttcc
     8881 gtccagaatg cgcggtcatc accgtcaata cagccccttc cgcagacagt tacattcacg
     8941 gcgttcagag catacagaat cgcatagcgc gagttgagag cgttgtcgag cagttccgcc
     9001 cggtagttcg aatccggaag gttgggaatg ggcggataat cccttttctc ccgcgagggc
     9061 ttgaccaagg ctcccgctgc aaggtgaaag gttacgttac tgcgaagaag aaccctgccg
     9121 attacgtatt caccgggggg aacgaaaacg gtacctccgc cggattcggc acacgcgtcg
     9181 atggccttct gaaatgcgtg tgtgcttgtt tccggcgcat gtgagcgcgc cccgaacgat
     9241 gcaacatcga aaagacgaga tgtgtccatc gcgcgcgtac tcctgtggac tcttggcacc
     9301 actggtggct ggcttcagac tcgggggaac gtcccctccg ggttcccgca ggcttcgtgc
     9361 agcaaggtca tgaatcggca caaattgcgg aacgtgacag gaggttgaat accgtgatcg
     9421 ccgttaggga agtagcgtcc gcgtgcaagc aactgaggaa cttttgacag tatttccggt
     9481 tcgatgaggg cttcatttcc ctcattgact acttcctttt ccaaccagcc gaagaagacg
     9541 aattcggggt gattctgccg aagttcaaac aggtcgtttc cagccttgac ttcgtacggg
     9601 aatatcgcgt tgacgccgta ttcggaggcg acgccggtaa attccatggc gttcccatcg
     9661 gtatcaatgg ccacaatctc cgcgccattc gcgaaggcac aatcgcagac ctcacggtag
     9721 tattcaccgc agaattcccg aaaatggcgg ggagaaagca gcatgccgtg attgaagcaa
     9781 agatcctccc ccatctgcac aacttccggt ctgagccttt ctatcaacgg gaacacaaat
     9841 tccctgtggt atttcagccc cgtttcaatg atgtcgtgcg caagttcggg atcctcgatc
     9901 aaggtcacac tggcgccttc ggttcccata aggccgcgta tatgcccgta cgtgctcccc
     9961 gctccgacac acagcggcct gtcgcgcgtt tcatacctgc ggcagttcgc ctccatttcc
    10021 tcgggcgaca ttacccgccg ggagcgcgtg cgttctttgt aaaactccca ggattcgcgg
    10081 tctcggacgg ggtacctgac aaactcaggc atgctgtagg tgacgtcatt ttccaccacc
    10141 tgccgcgtga tttccccgct ttcggactcg atgatctcaa aaccgttctc
//
LOCUS       DAOTNG010000082        10108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6218_length_10108_cov_5.079379, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000082 DAOTNG010000000
VERSION     DAOTNG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_10108_cov_5.079379"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="P5179_05515"
     CDS             complement(<1..273)
                     /locus_tag="P5179_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRS94709.1"
                     /translation="MPRKRTIEIYDSTLRDGAQALGINYSVEDKLLIAHRLDELGVHF
                     IEGGWPNRTNRGDVEFFQRARSAKWKNATIVAFGSTHRAGAAARDDA"
     gene            complement(580..729)
                     /locus_tag="P5179_05520"
     CDS             complement(580..729)
                     /locus_tag="P5179_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94710.1"
                     /translation="MVLLVFCGILLLVTAKLAGLVFLLGGWVIKLLAVLAVPIWFLPA
                     RRRPT"
     gene            complement(757..1518)
                     /locus_tag="P5179_05525"
     CDS             complement(757..1518)
                     /locus_tag="P5179_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRS94711.1"
                     /translation="MGKIPVALQLYSLRDVIPYDVEGTLASVAKMGYQGVEFAGYYGK
                     SAKELRGILDDNGLLVAGTHIGLETLLGDEFDKTVEFNQELGNKFLIVPGLAEERRNS
                     VKAWKDTAKIFNDLAEKVKPCGMRVGYHNHWDEFKPMEGQLPFDVFFSATVADVVMQL
                     DTGNALYGGAESVPLLKKYPGRAATVHLKECSRNEEVNFASMYGEGSVPWKEVFELCE
                     TVGKTEWYIVEQEKYLSPPLESVAEGLQALRKLGV"
     gene            complement(1603..2769)
                     /locus_tag="P5179_05530"
     CDS             complement(1603..2769)
                     /locus_tag="P5179_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94712.1"
                     /translation="MAERVTIGMIGCGGMMGAHERGFRELWDKGIRNFEIVATCDLVK
                     SLAEKMADQVGGWQGKRPRVYSDVETMLKSEPDMMAVDVVTVHRAHHSVVVPCFEAGK
                     HVTVEKPLAITLRAGKLMLDAAEKAGKVFQVAENYRRSTENRAIKWAIDSGMIGKLRM
                     IYWVDVEERLWYWTWRDHRDQAGGGWPLDGGVHFADLFRYHVGDPESLYCTVRTYNPF
                     RYKDTDNLTGPIPVDVEDTTLAVLHFADGVTGSWTYTLSGPGDKWGHRAVYGEHGSLT
                     WGSGFKSRKTSMTMEELIAEHHRAIGPDGVEKLFPRGIQDTIATELNEFVEAVLHGTP
                     IEITGWEGYKDQAISMALYESDAAGRPVLLSDVEALRLEAYQGRLNADLEGLTQ"
     gene            complement(2862..5336)
                     /locus_tag="P5179_05535"
     CDS             complement(2862..5336)
                     /locus_tag="P5179_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS94713.1"
                     /translation="MVDKSNPKAYTVHMIGNAHIDPIWLWRLEEGRKEVSDTCRSALD
                     RMKETPGFIFVRSSASSYVWLEEDAPDMFEEVRQRIEEGRWEIVNGWWEQPDCNIPGG
                     ESYVRHALYAKEYFMEKFGVEPTVGYNVDTFGHNGNLPQILAKSGFKSYIFFRPGEHE
                     KHLPQHFWWESPDGSRVLGIRPPHHYGFGGNVEEIKKRIVTAWEQTDDGVTEVMCFYG
                     VGNHGGGPTKLNIQGILETDAMPDAPNAKFARCDAYVEKVLAQKTDFPVVKDDLQIHA
                     VGCYTAVSAVKVLNRRAEQKLMAAERFATVANLVSHKCYPGAELRDAWRDVLFNQFHD
                     VLAGTSIHPAYEDTYAMFAKAIETADVSQRASLRAIATNVDTRGKNLALVAFNPNPWW
                     RRDLITADVEVAVVPKLVRLVADDDSEVPVQIAGVSEAGDKSTVTVMFVAELPALGFR
                     VFRLHLDEMPSFETPMVCAHPNGLSNDVLSLVVDPYSGGLVSLRAGNGPELIAAPGAN
                     FVVIKDHSDTWSHGITEFRNELGRFIANGRIRVVSQGPVRATLEVVSSFEDSTLVQHF
                     HVYPGINRVDVDVKMDFRERHRMVKLAVPTALDQITATAEIPYTYIERDWNGEELPGQ
                     KWIDVTGKVDRARFGVTMLNDGRYGFDVKESEMRMSVLRTPIYAFHDPRKVNPKETYQ
                     YPDLGTTSFRYALIPHGGDWRAIMAPRAGEEFNTPTVAFIEPHHNGALKSGAEFLEVG
                     PDNVVGMILKRTQEYANGNAADDRIIVRLYEGHGLDGIARVRLPFLGIAAEVPIGHHE
                     IKTLAFDVQNPSSAPVEVDLLERPIP"
     gene            complement(5464..6204)
                     /locus_tag="P5179_05540"
     CDS             complement(5464..6204)
                     /locus_tag="P5179_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS94714.1"
                     /translation="MLRGVRAVLFDYGGTLGNDEPAYAQGFTHLLTAMGYPTDMALYR
                     IASRDAEAALPPAPRDAEAYLTWRAQYRRELLRFCGVPRRELPRMEDAVAKRLRYFTT
                     LFAYAESRFVLRSLKWAGFTVGMISNISPLLPMVNKELGFEEYLDFAIASETFGCEKP
                     DRRIFEEGLRLAGVPAEKAVYVGDSPEADVAGALSVGMHPVLIARRYPLPEVAEGVTA
                     ILNLTQLFDLLGIDPWDEPSLQTPSLTR"
     gene            6379..7281
                     /locus_tag="P5179_05545"
     CDS             6379..7281
                     /locus_tag="P5179_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS94715.1"
                     /translation="MHVPQTVTYKIADRCDLKADVYVPERGDAAAVILWIHGGALIGG
                     NRKDIREEQLEFYLRNGYALVSIDYRLAPETKLPEIIADIRDAYDWLHREGQQRFGLD
                     TRRIGVAGHSAGGYLTLMTGFCVSPRPRALVSFYGYGDLTGGWYSRPDPFYCKLAPVE
                     KETAHASVGTEPVADLSLPGTLPDPRGRFQFYLYCRQQGLWPKEVSGFDPDSESKQYV
                     PFNPLWNVTPDYPPTLLLHGDLDTDVPYAQSVLMAYEMQRHGVEHELHTVRGGGHGFD
                     GHGFGKPEVDTAFDRVIGFLKQHL"
     gene            complement(7297..9534)
                     /locus_tag="P5179_05550"
     CDS             complement(7297..9534)
                     /locus_tag="P5179_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HRS94716.1"
                     /translation="MIIAEHGKPRCRILLGTRAAWLERHAAEELQRYLARMANALIPV
                     EEEPVVSGPVLAIGRPERNPFVARAIASGAVSAPPSEESDGFVIQTVEIDDAESLVIA
                     GSSDRGTLYGVYWLLEEVLGVGFFRDGERVPNLTTVELPPLSISERPHFPERHEGNGC
                     VFTYSTSSWGVDEWKWELDWKAKRRFNTTWPFDVAHDLLPRMLADWGVDTQVPRRSEG
                     HTVHEQAFRYARSLGIRIPCIIGNLPATPELRARFPETRVLVLQYSELPPYTIVHPGD
                     PLFRRYVVEYVRRYNDSWGSDHLYIASFISESKVLEGAANLHEARLDFARAVSEALKE
                     ADPEATWIIETWSFDMDAEDVNHRWSPAQIKEYLGTVSVPHIVADLWSEERGKYLQTE
                     NFFGHPWGFGVLHSFGAGSYLHGDVSLLLKKVRDVVNQPDGKNCRMFLLQPEIIQFNS
                     FFYEFCAQVAWDPLRGSVESFVERYCRKRYGASLGEKLREPWNILVDTVYGSESGSVG
                     TITDPLYWYRPDIHLLLGWTKPDDKTATLRARRHLLIPKLRDVLELFLTQEVEVRTDP
                     LALRDFVDVARQWIAERFNIAIISARDAFLAGDEVELEKRRAECVALLDDQTRLLSSW
                     PPYRLDTKVKEHRARFGDDAERWVKHTHVWVYGDEGMHSPPLRDYYRMDLDGLIADYY
                     RPRVTAYFDLLHSKLNSGNQCITPEEFDSAYQPIEEAFVSHTMRRLPAGEDPLAIVRG
                     LLVGQ"
     gene            9636..>10108
                     /locus_tag="P5179_05555"
     CDS             9636..>10108
                     /locus_tag="P5179_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS94717.1"
                     /translation="MTDSTHNRHIHQSCLEHLHLHLAVRVSPSSRPSLLRLFGTLAVG
                     TIAISTSAPFIKLAGMRPFALASGRLLGVAILYALVGRDLFSAWRGLERRERARAIVA
                     GMFLAAHLGCWIWAFEFTDLPSAVLLLVLQPLFAAWFGSFVFREKVTSSAITAVIL"
BASE COUNT         2081 a         3129 c         2721 g         2177 t
ORIGIN      
        1 cgcgtcgtcc ctggcggcgg ctccggcacg gtgagtgctt ccaaacgcaa cgatggtggc
       61 gttcttccac ttcgcactcc tcgcgcgctg gaaaaattcc acatcgccgc ggtttgtgcg
      121 gtttggccac ccaccctcaa tgaagtgcac gccgagttcg tccagtctgt gtgcgataag
      181 caacttgtct tccacggaat agtttattcc gagtgcctgg gcaccgtcgc gcagagtgga
      241 atcatatatc tcgatagttc gttttctggg catcgtcttc tccggggaat cagaaaaacc
      301 gttacaacac gcggaacggg agtacacaaa caagcgaggt gcacaatgcg cacctcgctt
      361 gcacggcgga tgagatccgc cagattacac gatgcccacc ggacggcgca aatgatcccg
      421 gccgtccgga tgagtacctc cagcagttgc tgcgcaaggg aacgtgttca tttcacaggc
      481 ttttcgaatg agtagttgtc caaagcgacg ttagaatata cttttcgagt ggttgatcag
      541 caagaatcgt cgcgcatctc cctcgaaaac aagacgtcgt cacgtggggc ggcgccgtgc
      601 cggcagaaac cagattggaa ccgccagcac ggccaggagt ttgattaccc acccgccgag
      661 aaggaacacg aggcccgcca gttttgccgt caccaagaga aggatgccgc agaacacgag
      721 caacaccatc acggcatacc ctgtcggccc catcggttac acacccagtt tcctgagcgc
      781 ctgtaaccct tccgcgacac tttccagtgg cgggctcagg tatttttcct gctcaacgat
      841 gtaccactca gttttgccga ccgtttcgca gagctcaaat acctctttcc acggcacact
      901 gccctcaccg tacatcgacg cgaaattcac ctcctcgtta cgggagcatt ccttgaggtg
      961 cacggttgcc gcacgtcccg ggtatttctt gaggagaggc accgactctg cgccgccata
     1021 caaggcgttt cccgtgtcaa gctgcatgac gacatcggcc acggtggcgg agaagaaaac
     1081 atcgaacggg agctgcccct ccatgggttt gaactcgtcc cagtgattgt ggtacccaac
     1141 acgcatgcca cacggtttca ccttttccgc caggtcgttg aagattttgg ccgtatcctt
     1201 ccaggccttc acggaattgc gccgttcctc ggcaagtccg gggacaatga gaaacttgtt
     1261 cccaagttcc tggttgaact ccaccgtttt gtcaaactcg tccccgagca gcgtttcgag
     1321 accgatgtgc gtaccggcga ccagaagacc gttgtcatcc agaattcccc gcagttcttt
     1381 tgcgctcttt ccgtaatagc ccgcgaattc cactccctgg taacccattt tcgctaccga
     1441 ggcaagggtg ccttcgacat cgtacggtat gacgtcacgc aatgagtaaa gctgcagagc
     1501 gacgggaatc tttcccatgg aacacctcct tgttttggcg cacgtgttga cacccatctg
     1561 gaatgtggag agcagccaca gaccgatgtg tggcgcatac agttactgag tcagaccctc
     1621 aagatcggcg ttgagacgcc cctgataggc ttccaagcgc agcgcttcta catcgctcaa
     1681 cagaacaggc cgtcccgctg catccgactc gtacaacgcc attgagatcg cctggtcctt
     1741 gtacccttcc caccccgtga tctctatggg tgtgccgtgc agcaccgctt cgacgaattc
     1801 attgagctcc gtggcgatgg tatcttgaat cccgcgcggg aagagctttt cgaccccgtc
     1861 cgggccgatg gctcgatggt gttccgcgat aagctcctcc atcgtcatgg atgtttttcg
     1921 cgatttgaac cccgaacccc acgtcagtga cccatgttca ccgtagaccg cgcggtgtcc
     1981 ccatttgtcc ccgggacctg agagggtgta cgtccacgat ccggttaccc cgtcagcaaa
     2041 gtgcaacacc gcgagcgtgg tgtcctccac gtcaaccggg atggggccgg tgaggttgtc
     2101 ggtatccttg taccggaacg ggttgtaggt ccgcaccgtg cagtacaggc tttccgggtc
     2161 tccaacgtgg taccggaaca ggtcggcaaa gtgcacgccg ccatcgagcg gccacccgcc
     2221 tccagcctga tcgcggtggt cgcgccatgt ccagtaccag agcctctcct caacatccac
     2281 ccagtagatc atcctcaact tgcctatcat tccagaatca atcgcccatt tgatcgcacg
     2341 gttttccgtg gaccggcgat agttctccgc cacctgaaag acttttcctg ccttctcggc
     2401 ggcatccagc atcagcttgc cagctctgag tgtaatcgcg agcggcttct ccactgtaac
     2461 gtgttttccg gcttcaaagc acggcacaac caccgaatga tgcgcacggt ggaccgtaac
     2521 aacgtccacc gccatcatat cgggttcgga tttcaacatg gtttccacat cagaatagac
     2581 acggggccgc ttcccctgcc agccaccgac ctgatccgcc atcttttcgg ccagcgattt
     2641 gacaaggtca caggtagcta cgatttcgaa attcctgatt cctttgtccc ataactctcg
     2701 aaatccccgc tcgtgcgcgc ccatcatgcc gccgcacccg atcattccaa ttgtcacccg
     2761 ttctgccatc tttccacctc tcgatgtgtt accgtccact ttcgccgtgc cgtacccagg
     2821 gcgcgtgtag aactcaccgt caccatcctg cgcgtcgtcg atcaaggaat cggtcgctca
     2881 agcaggtcta cctcgaccgg ggcagacgag gggttctgaa cgtcaaacgc gagcgttttg
     2941 atttcgtgat gtccgatcgg tacctcggcg gcaatcccga ggaatggcaa tcgcaccctg
     3001 gcaattccgt caagcccgtg cccttcgtac aaacggacaa tgatcctgtc gtcggctgcg
     3061 ttcccgttcg catactcctg cgtgcgcttc aggatcatcc cgacaacgtt gtccggacca
     3121 acttccagga actccgcccc gctcttcaac gctccgttat ggtgcggctc gatgaacgcc
     3181 acggtcggag tgttgaattc ctcaccggcc cgcggagcca tgattgcgcg ccagtctccc
     3241 ccgtggggga tgagggcata ccggaatgag gtcgtgccga gatccggata ctgatacgtt
     3301 tccttcgggt tgacctttcg cggatcgtga aacgcgtaaa tcggcgtcct caggacgctc
     3361 atccgcatct ccgattcctt cacgtcgaag ccgtaccgcc cgtcgttcaa catggtaacg
     3421 ccaaaacgcg cccgatccac cttccccgtt acgtctatcc acttctggcc cggaagttct
     3481 tcaccgttcc aatcccgttc gatgtacgtg taagggatct ccgccgtcgc cgtgatctgg
     3541 tccagagcag tcgggacggc cagcttgacc atacggtgcc gttcgcggaa atccattttg
     3601 acgtctacgt cgacgcgatt gatgcccggg tagacgtgga aatgctggac taacgtacta
     3661 tcttcgaagg aacttaccac ctcgagtgtc gcccggaccg gtccctgaga caccacacgg
     3721 attctgccgt tcgcgataaa cctccccagc tcgttcctga actcggttat cccgtggctc
     3781 caggtgtcgg aatgatcctt tatcacgacg aagttcgcgc cgggcgcggc aatcaactcg
     3841 ggaccgttgc ccgctcgcag gctgaccagc ccacccgaat agggatcgac gaccagcgat
     3901 agaacgtcgt tggacaagcc gttggggtgc gcgcagacca tcggtgtctc gaacgagggc
     3961 atctcgtcga ggtgcaacct gaacacccgg aaccccagcg caggcagttc ggcgacaaac
     4021 atcacggtaa ccgtgctttt gtcacctgcc tccgaaacac cggcgatctg aacgggcact
     4081 tcgctgtcat cgtcagctac cagacgtacc agtttgggga ctaccgcaac ctcgacatcc
     4141 gccgtaataa ggtctctgcg ccaccacggg ttcggattga aggcaaccaa ggccaggttc
     4201 ttcccgcgcg tatcaacgtt ggtggcgatt gcacgcaaag aagccctctg cgagacatcc
     4261 gccgtttcga tcgccttcgc gaacattgcg tacgtatcct cgtacgcggg atggatcgag
     4321 gtgccggcca gcacgtcatg gaactgattg aacagcacgt cacgccatgc gtcacgaagc
     4381 tctgcacccg gatagcattt gtgagaaacc agattcgcca cggtggcaaa gcgctcggca
     4441 gccatcagtt tttgttccgc gcgccggttc aagaccttca ccgcgcttac tgccgtgtaa
     4501 cacccgaccg cgtgaatctg cagatcatct ttgacgacag gaaaatccgt tttctgcgcg
     4561 agaacctttt ctacatacgc atcgcaccgt gcgaacttcg cgtttggcgc gtccggcatc
     4621 gcgtccgttt ccagaattcc ctgaatgttc agtttggtag gtccgccgcc atggtttccg
     4681 acgccgtaga aacacatcac ctcggtaacg ccgtcgtcgg tctgctccca tgcggtaact
     4741 atccgctttt tgatctcctc gacgttcccg ccgaagccat agtggtgggg cgggcgaatt
     4801 ccgaggacgc gggaaccgtc gggcgattcc caccagaaat gctgtggaag atgtttttca
     4861 tgctcccccg gacggaagaa tatgtagctc ttgaagccgc tctttgcgag aatctgagga
     4921 aggttcccat tgtgtccgaa cgtatcgacg ttgtacccca ccgtgggttc cacgccgaat
     4981 ttttccatga agtattcttt cgcgtacagt gcgtggcgaa cgtacgactc cccaccgggg
     5041 atattgcagt caggctgctc ccaccagcca ttaacaatct cccagcggcc ctcctcgatt
     5101 ctttgacgaa cctcctcgaa catgtccggc gcgtcttctt cgagccacac gtacgatgac
     5161 gcagagcttc gaacgaagat gaatccggga gtttccttca tcctgtcgag agcggaacgg
     5221 caggtatcac ttacctcctt acgaccctct tcgagccgcc acaaccatat cgggtctata
     5281 tgcgcgttgc caatcatgtg aactgtgtaa gccttgggat tcgacttgtc caccatcggg
     5341 gattccttct gctacgtagt cctggatgca gtgcgccacg cacgacggaa gatgaagcgg
     5401 gtcgcacaga agcgaaaaat cggtctttgg atcggtgtga tgatccggtg tcctttcgat
     5461 tgatcagcgg gtgagagatg gcgtttgaag cgagggttcg tcccaggggt caatccccag
     5521 aaggtcaaat aactgcgtaa ggttgagaat tgccgtgact ccctcggcga cctcgggaag
     5581 cgggtagcga cgtgcgatca gaacggggtg catgcccact gacagggccc cggcaacgtc
     5641 ggcttccggg ctatccccga cgtaaaccgc cttttcggca ggaacgccgg caaggcggag
     5701 gccttcctcg aaaatgcgcc ggtcgggctt ttcgcacccg aatgtttctg atgcaatcgc
     5761 gaaatccaga tactcttcaa aaccaagttc cttattgacc atcggcagga ggggcgagat
     5821 gttggagatc atcccgaccg tgaatcctgc ccatttgagg cttcgcaaca caaaacggga
     5881 ttccgcgtac gcaaacagcg tggtgaagta ccggagtcgc ttcgccactg catcttccat
     5941 cctcgggagc tcgcgcctcg gaacaccaca gaagcggaga agctcccgcc ggtattgtgc
     6001 ccgccaggtg agataggctt ccgcgtcccg cggcgcagga gggagagccg cttccgcgtc
     6061 acgacttgcg atccggtaaa gcgccatatc cgtcgggtac cccatcgcgg tcaggagatg
     6121 ggtgaaaccc tgcgcgtagg caggctcgtc gttcccgaga gttcccccat aatcgaaaag
     6181 aaccgcgcgc actcctctca acatcttctg attgtctcca catctaagac aggttcaaat
     6241 ggaaaaatcg tcgcattcgg catgtcccgt ttccgccggt tgcgtacgaa cgcacatgtg
     6301 ttttgattcc ttgaagacag gaccatccag aagataagtg ctccacttgg acgggtgcac
     6361 cccgcggaaa gagaccacat gcatgttccc cagacagtaa cgtacaagat cgccgacaga
     6421 tgtgacctca aagccgacgt gtatgtaccc gaaagaggcg atgccgccgc ggtgattctg
     6481 tggattcacg ggggcgcatt gatcggcgga aacaggaaag acatcagaga agagcaactg
     6541 gagttttatc tgcgtaacgg gtacgctctc gtttcgattg actaccggct cgccccggaa
     6601 accaagctcc cagaaatcat cgcggatatc cgcgacgcgt acgactggct tcaccgcgag
     6661 ggacaacagc gcttcggtct cgacacaagg aggatcggtg ttgcgggaca ttctgcagga
     6721 gggtacctca cccttatgac ggggttctgc gtctcaccgc ggccccgggc gctcgtttcc
     6781 ttctacggat acggcgacct gacgggtggc tggtacagtc gtccggaccc tttttactgc
     6841 aaactggcgc cagtggagaa agagaccgcg cacgcctccg tcggaaccga accggtagcc
     6901 gatctgagcc tgcccgggac attgccggac cccagaggtc gcttccagtt ctacctctat
     6961 tgccgccagc agggtctctg gcccaaagaa gtcagcggct tcgatccgga ctccgagtcc
     7021 aagcagtatg tgccgttcaa ccctctctgg aatgtgactc ctgactaccc gccgacgctt
     7081 ttgctgcacg gcgatctcga cacagacgtg ccgtacgccc agtccgtcct catggcgtat
     7141 gagatgcagc ggcatggcgt cgagcacgaa ctgcacaccg tgcgaggtgg cggccacggg
     7201 tttgacgggc acgggttcgg caaacctgag gtcgataccg cattcgaccg cgttatcgga
     7261 ttcctgaaac agcacctttg agtgcgtcag gtggcgtcat tgacccacca gtagcccgcg
     7321 aactatcgcc agagggtctt cccctgctgg caagcggcgc atcgtgtgcg acacgaacgc
     7381 ttcctcgatg ggttggtacg ccgagtcaaa ctcttcaggt gtgatgcact ggttgccgga
     7441 gttgagcttg ctgtgcagca ggtcgaaata ggctgtcaca cgtggtctgt aatagtccgc
     7501 gatgaggccg tcgaggtcca tgcggtagta atcccgcaac ggcggtgaat gcatgccctc
     7561 gtctccgtaa acccagacat gtgtgtgctt tacccaccgt tccgcatcgt ccccgaatcg
     7621 agcgcgatgc tccttcacct tcgtgtcgag tcgatagggc ggccaactgg ataacaacct
     7681 ggtctggtcg tccagcaatg ccacgcactc tgccctgcgt ttttccagtt cgacttcgtc
     7741 gcccgcaagg aacgcgtctc gtgcggagat gatggctatg ttgaagcgct ccgcaatcca
     7801 ttgtcgggca acgtcaacaa aatcgcgcag cgcgagcggg tcagttctca cctccacctc
     7861 ttgcgtaagg aaaagctcaa gcacgtcgcg aagtttcggg atcaacaggt gcctccgtgc
     7921 ccgcaacgtt gccgtcttgt catcgggttt ggtccatccg aggagaaggt ggatatccgg
     7981 gcggtaccag tacagcggat ccgtaatcgt gccgacactt ccgctttcgg acccgtaaac
     8041 cgtatccacg aggatgttcc agggttcacg caacttttcg ccgagagatg caccgtagcg
     8101 tttccggcaa taccgttcga cgaagctttc aacactgccc cggagaggat cccacgcaac
     8161 ctgcgcgcag aattcataga aaaagctgtt gaactgaatg atttccggct gaaggagaaa
     8221 catccggcag ttcttgccgt ccggttggtt gaccacgtcc cggacctttt tcagcagaag
     8281 actcacatcc ccgtgcagat agctgcctgc gccaaaactg tggagaacgc caaagcccca
     8341 ggggtggccg aaaaagttct ccgtttgcag gtacttgcca cgctcttccg accagagatc
     8401 agccacgatg tgcggaactg agacggtccc gaggtattcc ttgatttgcg ccggagacca
     8461 tcgatggttc acgtcttccg cgtccatatc gaaagaccac gtctcgatga tccaggtggc
     8521 ttccggatcg gcttccttaa gggcttcgga gactgcacgg gcgaaatcca gccgcgcttc
     8581 gtgaaggttc gccgcacctt ccagaacctt actttcgctg atgaagctgg cgatgtaaag
     8641 gtggtcggag ccccacgagt cgttgtatcg ccgcacatac tccacaacgt agcgacggaa
     8701 aagcggatct cccgggtgaa cgatcgtata agggggaagc tcactgtact ggagaacgag
     8761 cacgcgtgtt tcgggaaacc gcgcacgtaa ctcgggagta gcgggaaggt tcccgattat
     8821 gcagggaatt cttatcccca ggctccgcgc gtagcgaaat gcttgttcat gaactgtgtg
     8881 cccctccgag cgtcgaggta cctgtgtgtc tacaccccaa tcggccaaca ttcgcggaag
     8941 caggtcatga gcaacatcga acggccacgt agtgttgaat cgcctcttcg ctttccagtc
     9001 caactcccac ttccattcgt ccacgcccca agaagacgta gaatacgtga acacacagcc
     9061 gtttccctca tggcgttccg ggaaatgagg tcgttccgag atcgaaagtg gtggcagttc
     9121 caccgtggtc agattcggca ctcgttcccc gtcccggaaa aaccccacgc caagaacctc
     9181 ttccagaagc cagtacaccc cgtaaagggt gcctcgatcg ctgctgcccg caatcacgag
     9241 cgattccgca tcgtcaattt ccactgtctg gatcacgaag ccatcggatt cctcagatgg
     9301 cggcgcgctc accgcacccg acgcgatcgc ccgggccaca aacggattcc tctcggggcg
     9361 gccgatagcc agcactggac cagacaccac cggttcttct tccaccggaa tcagagcgtt
     9421 cgccatgcgc gccaggtatc gctgcaactc ctcggcagca tggcgctcga gccacgcagc
     9481 tcgcgttccc agaagaattc tgcaacgtgg tttcccgtgt tcagcaatga tcatttctac
     9541 ctccggtgca aagcgtgcgg ccgcagcccg tttcatttga gagtataaga gccgcacaac
     9601 cacgctccca cacgagggag tgacaaaggc gggcgttgac cgactccacg cacaaccggc
     9661 atattcatca atcctgtctc gaacacctcc acctccacct ggcggtccgc gtgtctccat
     9721 cttcgcgtcc ttcactcctg aggttgtttg gtaccctggc ggtgggcacc atcgcaatca
     9781 gcacatccgc gccttttatc aaactggccg gaatgcgtcc cttcgcgctg gcgtccggaa
     9841 ggcttctcgg cgtcgcgatt ctgtacgcgc tggtcggcag agaccttttc agcgcgtggc
     9901 gtggacttga gcgccgtgaa cgcgcacgag ccattgtcgc cggcatgttc ctcgccgccc
     9961 atctggggtg ctggatctgg gcattcgaat tcacggacct tccgagcgcg gttctgctgc
    10021 tggtgcttca gccccttttc gccgcgtggt tcgggtcgtt tgtgttccgg gaaaaagtta
    10081 cttcctccgc gattaccgcc gtcatact
//
LOCUS       DAOTNG010000083        10000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6277_length_10000_cov_5.471996, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000083 DAOTNG010000000
VERSION     DAOTNG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_10000_cov_5.471996"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="P5179_05560"
     CDS             complement(<1..635)
                     /locus_tag="P5179_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94718.1"
                     /translation="MQESLHPLDPSPFGVVAMLRTRPEELEGAARLAVEAGVRWTRED
                     ILWHQVQPERTAWRWERYDRELAVLKARGINVLGLLCYGTPWAMSLRNANGQPDIWSL
                     PDLSAWADYVSAVVERYRGQIHAWEIWNEPNSQTFWHPRPDPREYARLLIAGAEAVKR
                     ADPSAWVLGCNTGDVDPWFHRAVFDEGGWGHLDIIGVHPYRPPHTPERTDMV"
     gene            complement(646..1116)
                     /locus_tag="P5179_05565"
     CDS             complement(646..1116)
                     /locus_tag="P5179_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94719.1"
                     /translation="MSPILPCVLLLCMLPPANAQTKPADPDTAGIQIKLPPDSYYERG
                     RLAGIRLYQTRKAAAGGYAAGLFGPPGWGVGWIALKAMKPAHVPSAQIAELDSSQMAD
                     FTRGYTDYVREARNAAFVSGAVKGTTTTAVVLVVGGLVMSVFLFVILSALTASG"
     gene            complement(1142..>1829)
                     /locus_tag="P5179_05570"
     CDS             complement(1142..>1829)
                     /locus_tag="P5179_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94720.1"
                     /translation="YPDIIDPHTCNMKIGPGVFGFNTGHVYGVDNTSPESISKALLHG
                     RKMTAQYRQAFADLHPAFRNAFLAATGSQMGIRETRRIIGDYKITVDDYLARRSFPDE
                     ICRNAYGIDVHGQKNRIGALTNAGIDAFKKANEELTRPYEKGESFGVPYRCLTPKGLK
                     NVLVGGRCISADRQANGSIRIMACCLNTGEAAGIGAAMAAKDTRDVHGVNTDELRAKL
                     KAFGAYLPTA"
     assembly_gap    1830..1929
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1930..2264)
                     /locus_tag="P5179_05575"
     CDS             complement(<1930..2264)
                     /locus_tag="P5179_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94721.1"
                     /translation="MPEYALKPCSIPLIDTYDVIVAGGGPAGCAAATAAAREGARTLL
                     LESDSALGGMGTLGLVPWFCGYHDREKIIARGLAMHVHDRLRENMPHFAEWLAKNPLH
                     DPPIDPELL"
     gene            2522..6091
                     /locus_tag="P5179_05580"
     CDS             2522..6091
                     /locus_tag="P5179_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94722.1"
                     /translation="MFRSTPSARRWCVLTAALVLSSVPFLRAGTTGKITGTVRQPGGQ
                     PIPGVAVQISGLRLGSITDAEGRYFILQVPPGTHQVEASMVGFRTAVTQNVSVSADRT
                     TMLNFTLQEETIEVEALVVTAGRPPIEADVTSSQTTVNARRVAEIPVNQMLDVLAYEP
                     GVSLSRDTELNIRGGGATQVRFQVDGLDRTDGLTNKPYTQLNQTLVAEVTLLTGGFNA
                     EYGNVRSGMVNVVTKDGTERGVGKGWVSGVFSGTPTARKKHFGPGAYDEAQFDYYTVM
                     RSDSSTRGPTGAADPYGAIYWPNLYESTRNDTAFRRVWTAAPNMYKVFDGWNARVTTA
                     NANNRGAGVYGKGTRTKPWTAAQMIEAWRYETNLNENVWGYGNKPDWSLDIAAGWGLP
                     HKLGGIVVGYVTNKEMTVVPALRPYYVDQTFDVKLTLTPIDRLKWSFSYMYGTSESTG
                     NGTAGYDPEMASTAGSVAIGSDPVSFRTASDLLGTVNQSKTAAGNNRLNLSYNSLLDG
                     SFWQWGTALTYTLSPHTFATAAFGQTRSKWDLQRDMPRADINDFSPTGSYRPPSSFSY
                     GAWLLMLIPLSDWSDINGDGTPDRPTSAEDATAPGRAYMGNPYGYGAAYPFVPSRTVF
                     VNRQMVFAPGDTARVVSPQGWAQDGYFDLSGTFWLGEGGTQWMTSNSVQTAVRGDVTH
                     VIGDHTIKAGAEFLLSDLEYHTEQDARLLGNRFSVNYRDYGGKYPAARPNILGVFLQD
                     KFESQGLIMNYGLRLERFDGGAPYYMPDLIYDDRLYSPLHGKYWFDSLLVARGWNVND
                     PAWGPLPGNQYEAWTKFVKNGVPEQFPMPWDVMNVLPQRDAKAHWRLAPRFGISHPVS
                     DRTKFFFNYGIFYSMAKPAHMYGTGLHDARIGGGGGRFEHMFNPELRPARTTSYEVGF
                     EHLFPLNFVLKTTGYTKANVDQVTGLQVRTGSGDGYRIYRNANYQDIRGLEIKLSRNG
                     RFISGWATYQIIATRSGQTGLEELRQNIAASSYYTPLVSTNSPADVVMASIRVGTPLE
                     WGLLRGGWGASILQSYSESTGEILYNPNAVPRRELPPEYVLRGRDSWGTSAKLTKDIS
                     LPRQRHVSLYLDINNLFNTKYLNGTYMNGNDYLAYIVERRAQGETDLRVGDPSTWDAL
                     TQPYRVKKADGTYTAWKAPISPRTDWLMFLYPRSYRAGIRFDL"
     gene            6228..7565
                     /locus_tag="P5179_05585"
     CDS             6228..7565
                     /locus_tag="P5179_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRS94723.1"
                     /translation="MYNEPLRPQFHFTARAGWLNDPNGLVFFDGVYHMFFQHNPFDTK
                     WGNMTWGHAVSTDLVHWRQVENAILPDRLGTAFSGSAAVDWRTTTGFGEAGVPPIVAM
                     YTSAGGTSPESAGQPFTQSLAFSADGGKTWEKFAGNPVLGHIVESNRDPRILWHEPTE
                     SWAMALYLDKNDYALFTSPNLKDWTRTCDVSIPDTSECPDFFPMAQDDDPAHVKWVFW
                     GANGCYLLGAFDGKTFVPEEGPCRLDSGANFYAAQTWSDIPEADGRRIQIAWMNGGQY
                     PGMPFNQQMSFPCALSLRSTSAGVRLCQMPVREIKCLREDSVIRTDVALLPGRLLDAS
                     AGELLDIEAEIEPWCAKAIEWSIRGNAIRYDAEKHLLSCCGREAPVDLAGGLLRLRIL
                     LDRTSLELFSGDGNVGFHQCFLPDAANTGVELAATGGEARIRSLAVHRLRSAW"
     assembly_gap    7719..7818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8067..>10000
                     /locus_tag="P5179_05590"
     CDS             8067..>10000
                     /locus_tag="P5179_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94724.1"
                     /translation="MTSAANTAQVTRSINCFRIGTPQWLSDERFAALLALFDRYPGVT
                     DEVTFFTSETHPPLPLEVLRDRCVLLSRRMDEVRRHGYQTGINILATIGHHEENLPNS
                     LDGPYTRMTDPDGRISRGVLCPNDEQAREYIREAYRLITEANPDYIWVDDDVRLFGHG
                     PILAGCFCDTCLAAFSRETRREWSRESLREAVNSGPLADRLMYRRAWLAHNRATIARL
                     FSLIEQTVHAIRPGLPIGFMTGERYFEGYDFDAWAEVLSGASAAPVRWRPGGGTYTDE
                     KLDNIVGKAHDIGRQVAFLPSAVTVIQSEIENFPYQRLKKSAKATALEAAAYIAAGCT
                     GAAYNVLSMYDEPLDEYEPLVRVLTENRPFLDALAKAGGRSSPVGLHSGWSKDSFATL
                     QLAEGDWLFPREGVPGGQTNEFHEIGIPPAYSLDAAQAVALTGNGVRAFDRRTLERIL
                     SGGVYLDAEAARALEEMGHAELVGFRVGEAFPIDAIEQFLPNPLCGAFAGRERDGRQS
                     FWKRTAYALEPMPGAEPLARLVDYGRKELAACGLGVFENSLGGRVCVAGYAPWTFLQN
                     LSTSSRTKAIVRWLSRETLPAYVESFHRIVLWVREPEPGKVAIALLNASLDPARQASL
                     LVRTAANELRLTDMRMQQATV"
BASE COUNT         1791 a         3223 c         3028 g         1758 t
ORIGIN      
        1 accatgtcgg tccgttcggg cgtgtgcggc ggccggtacg gatgcacacc aatgatatcc
       61 agatgacccc acccgccctc gtcgaacacc gcgcggtgga accacgggtc cacgtcgccg
      121 gtgttgcagc cgagcaccca cgcggagggg tcggcgcgct tgaccgcctc cgctccggcg
      181 atgaggagcc gggcatactc cctcggatcg ggccgcggat gccagaacgt ctgcgagttc
      241 ggttcgttcc agatctccca cgcatgaatc tgcccgcggt accgctccac taccgcgctc
      301 acgtagtccg cccacgccga gaggtcgggc aggctccaga tatccggttg accattcgca
      361 ttgcgcaggc tcatcgccca cggggtgccg taacacagaa gtcccagcac gttgatgccg
      421 cgcgccttca ggacggccag ctcccggtcg tagcgctccc accgccacgc ggtgcgctcc
      481 ggctgaacct ggtgccagag aatgtcctcc cgcgtccagc ggacgcccgc ctccacggca
      541 agccgggccg cgccttccag ttcctcgggg cgcgtgcgca acatggcgac cacgccaaac
      601 ggcgacggat caaggggatg aagtgactct tgcatgggca aatacctacc ctgacgcggt
      661 cagcgccgag agaatgacga acagaaacac gctcatcacc agccctccga cgaccaggac
      721 gaccgcagtg gtggtggttc ccttcactgc gcccgagacg aaggcggcgt tccgcgcctc
      781 tctcacgtag tcagtgtacc ctctggtgaa atccgccatc tgggaggagt caagctccgc
      841 gatctgcgcg gaaggaacgt gagccggttt catggccttc agggctatcc agccgactcc
      901 ccacccgggc ggcccgaaca gccccgcagc gtatccgccg gccgccgcct ttcgtgtctg
      961 gtagagccgt attccggcca gccgcccgcg ttcgtagtag ctgtcgggcg gcagtttgat
     1021 ctggattccc gcggtatcgg ggtccgccgg cttcgtttgc gcgttcgcag ggggaagcat
     1081 gcacaacaga agcacgcatg ggagaatcgg actcatcctt cacctcccgc agcagatccc
     1141 gtcacgccgt cggcagatac gccccgaacg ctttgagttt cgcccgcaat tcgtcggtgt
     1201 tcacgccgtg cacgtcgcgc gtgtctttcg ctgccattgc cgccccgata cccgccgctt
     1261 cgccggtgtt gaggcagcac gccatgatac gaatgctccc gttcgcctgc cggtcggcgg
     1321 agatgcagcg cccgcccacg agcacgtttt tcaagccttt cggcgtgagg cagcggtagg
     1381 gcaccccgaa gctctcgccc ttctcgtacg gccgcgtcag ctcctcattc gccttcttga
     1441 acgcatcgat ccccgcattc gtcagcgcgc cgattctgtt cttctgcccg tgcacgtcaa
     1501 tgccgtaggc gttccggcag atttcatccg gaaaactgcg ccgggcgagg tagtcgtcca
     1561 cggtgatctt gtaatcacct atgatacggc gcgtctcgcg gatgcccatc tggctcccgg
     1621 tggcggcgag aaaggcattc ctgaacgcgg ggtgcaggtc ggcgaacgcc tgccggtact
     1681 gcgccgtcat tttgcgcccg tggagcagcg ccttcgagat gctctccggg ctggtgttgt
     1741 ccacgccgta cacgtgcccc gtgttgaagc cgaacacgcc ggggccgatc ttcatgttgc
     1801 acgtgtgcgg gtcgatgatg tcggggtacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnt tcagcagttc cggatcaatg ggcgggtcgt gcagcgggtt ctttgccagc
     1981 cactccgcga aatggggcat gttctcgcgc aggcggtcgt gcacgtgcat cgccagcccg
     2041 cgggcgatta ttttctcccg gtcgtggtag ccgcagaacc agggcaccaa ccccagcgtg
     2101 cccatcccgc cgagcgcgct gtcgctttcc aagaggagcg tgcgcgcgcc ctctcgtgcc
     2161 gcggcggttg cggcggcaca accggcgggg ccgccaccgg caacgatgac atcgtacgtg
     2221 tcgattagtg gaatcgagca cggtttcagc gcgtattcgg gcatgtcagg gtccctttct
     2281 cccgcgggtg aggcgggatg tttgcgggtg atcgtgatga ctataacata gccgcgtggg
     2341 tgcatggtgg gaactgcgct ttgtacttgc gcgacggtgc gcgcacaacg cgggcagccg
     2401 agttgatggg gaactttgac cggggcggcg cttgaccccg cggaaggaaa aagcgagtat
     2461 atgcggatga tcaggctgtc acggcgtatg cgtcagttac ctgttcacca ggaggggtcc
     2521 catgtttcgg agcacgcctt ccgcccgccg ctggtgcgtt ctcaccgcgg cgcttgtact
     2581 ctccagcgtt ccttttctgc gcgccggaac cacgggcaaa atcaccggca ccgtgcgcca
     2641 accgggcggg caaccgatcc cgggtgtggc ggtccagatt tccggccttc gtctcggcag
     2701 tatcaccgac gcggaggggc gctatttcat tctgcaggtg cctcccggca cgcaccaggt
     2761 cgaggcaagc atggtcggct tccggaccgc ggttacgcag aacgtctcag tgtcggccga
     2821 ccgcacgacg atgctgaatt tcaccctgca ggaagagacc atcgaggtcg aggcactcgt
     2881 ggtgactgcg ggcaggcctc ccatcgaggc agacgtaacg tcctcccaga cgacggtgaa
     2941 cgcccgccgc gtagcagaga ttccggtgaa ccagatgctg gacgtgctgg cctacgaacc
     3001 cggtgtttcc ctttcccgcg ataccgagtt gaatatccgc ggaggcggcg cgacgcaggt
     3061 ccgtttccag gtggacgggc ttgaccgcac ggacggtctt acgaacaagc cctacactca
     3121 gctcaaccag acgctggtgg ccgaggtgac gcttctcacc ggcgggttca acgcggaata
     3181 cgggaacgtg cggtccggca tggtaaacgt ggtgacgaag gacggcacgg agcgcggcgt
     3241 tggcaagggg tgggtctccg gcgtgttcag cggaaccccg accgcacgga agaagcactt
     3301 cggccccggc gcgtacgacg aagcgcagtt cgactactac acggtcatgc ggtcagattc
     3361 gagcacgcgt ggcccgaccg gcgcagcaga cccttacggc gccatctact ggccgaatct
     3421 gtacgagtcg acgcggaatg acaccgcctt ccgcagggtc tggacggcgg cgcccaacat
     3481 gtataaggtg ttcgacggct ggaatgcccg cgtgaccacg gcaaatgcca ataaccgcgg
     3541 cgccggagtg tacggtaagg gcacgcgcac aaagccgtgg acggcggcgc agatgattga
     3601 ggcgtggcgg tacgaaacga acctgaacga gaacgtctgg ggatacggca acaagccgga
     3661 ctggagcctc gacatagcgg cggggtgggg tcttccccac aaacttggcg gaattgtggt
     3721 cgggtacgtc accaacaagg aaatgactgt cgtccccgca ttgcggccgt attacgtcga
     3781 ccagacgttc gacgtgaagc tgacgctgac gccgattgac cggctgaagt ggagcttttc
     3841 gtacatgtac ggcacgagcg aatcaaccgg gaacggcacg gccgggtacg acccggaaat
     3901 ggcctccacc gcgggttccg ttgccatcgg gagcgaccct gtctccttcc gcactgccag
     3961 cgatcttctc ggaaccgtga accagtcaaa aaccgccgcc ggcaacaacc gtctgaatct
     4021 ctcttacaac agccttctgg acgggagttt ctggcagtgg ggtaccgcgc tgacctacac
     4081 attgagccca cacaccttcg ccacggccgc attcgggcag acgcggtcga agtgggatct
     4141 ccagcgggac atgccccggg cggacatcaa cgacttcagc ccgaccggtt cgtaccggcc
     4201 cccgagcagc ttcagctacg gcgcgtggct gctcatgctg attccgcttt ccgactggtc
     4261 cgacattaat ggcgacggaa cgcccgaccg gcccacaagc gctgaagacg ccactgcgcc
     4321 gggaagagcc tacatgggca acccgtacgg gtacggggcg gcgtatccat ttgttccctc
     4381 gagaaccgtg ttcgtgaacc gtcagatggt gtttgcaccc ggcgacacgg cgagggtggt
     4441 gtcgccccag gggtgggctc aggacgggta tttcgatctg agcggcacgt tctggctggg
     4501 cgagggcgga acacaatgga tgacttcgaa ctccgttcag accgcagttc ggggggatgt
     4561 tacgcacgtc ataggcgacc atacgatcaa agcgggtgcg gagttcctcc tgagcgatct
     4621 ggaatatcat accgaacagg atgcgcgcct cctcggaaac cgtttcagcg tcaactaccg
     4681 cgattacggc ggcaagtacc cggccgcgcg tccgaacatt ctgggtgtgt tcctgcagga
     4741 taagtttgag tctcagggac tcatcatgaa ctacggcctc cggctggaac ggtttgacgg
     4801 cggcgcaccc tactacatgc ccgacctcat ttacgacgac cgcctttaca gcccgctgca
     4861 cgggaaatac tggttcgaca gcctgcttgt cgcgcgagga tggaacgtga acgatccggc
     4921 atggggcccg ctgccgggca accagtacga ggcgtggacg aaattcgtga agaacggggt
     4981 accggagcag ttcccgatgc cgtgggacgt gatgaacgtg ttgccccagc gcgacgcaaa
     5041 ggcgcactgg cggcttgctc cccggtttgg aatctcgcat ccggtcagcg accgcacgaa
     5101 gttcttcttc aactacggca tcttctactc catggcgaaa ccggcgcaca tgtacggcac
     5161 cggactgcac gatgcgcgca tcgggggagg aggcgggcgg tttgagcaca tgttcaatcc
     5221 ggagctgcgc cctgcacgca ccacgtcgta cgaggtcggc tttgaacacc tgttcccgct
     5281 gaacttcgtg ctcaagacca ccggctacac gaaggcgaac gtggatcagg tcaccgggct
     5341 ccaggtgcgc accggcagcg gcgacgggta ccgcatttac cggaacgcga actaccagga
     5401 cattcgcggg ctggagatca agttgagccg taacggcagg ttcatcagcg ggtgggcaac
     5461 ctaccagatc atcgccacac ggagcggcca gaccgggctg gaggaattgc gccagaacat
     5521 tgcggcgtcg agctactaca cgcctctggt ttccacaaac tcgccggcgg atgtggtgat
     5581 ggcgtcgatc cgcgtgggaa cgcctctgga atgggggctg ctgcgcggcg ggtggggcgc
     5641 gagcatcctg cagagctatt ccgagagcac cggagagatt ctgtacaacc cgaatgccgt
     5701 tccccgacgg gagcttccgc ctgagtacgt gctcagaggg cgcgattcgt ggggcacaag
     5761 cgcgaaactc acgaaggaca tctctctccc gcgccagcgg cacgtctcgc tgtacctcga
     5821 tatcaacaac cttttcaaca ccaagtacct gaacggcacc tacatgaatg gcaacgacta
     5881 ccttgcctac atcgttgaac ggagagcaca gggggagacc gacctgcgcg tgggcgatcc
     5941 ttccacgtgg gatgcactga cccagcccta ccgcgtgaag aaggccgacg gcacctacac
     6001 ggcatggaaa gcgcccatca gcccgcgcac cgactggctc atgttcctgt atcccagatc
     6061 gtaccgcgcg ggtattcggt tcgatctgta ggacgcggtt agcggttgaa gaaaagggcg
     6121 gccgtgcgtt ccagcacggc cgcccttttc tggcactgcg cgccatcaca gattcttctt
     6181 tgacgttgga tacatagggt attcgcgatt cgcagaacga ggtatacgtg tacaacgaac
     6241 ccctccgccc gcaattccat ttcaccgccc gcgcgggctg gctcaacgac ccgaacggac
     6301 tcgtgttctt cgacggcgtg taccacatgt ttttccagca caacccgttc gatacgaagt
     6361 ggggcaacat gacgtggggg cacgccgtca gcaccgacct cgtccactgg cggcaggtgg
     6421 agaacgctat tctccccgac cggctcggaa cggcgttctc cggctccgcc gcggtggact
     6481 ggcggacaac cacgggcttt ggtgaggccg gcgttccgcc catcgtcgcc atgtatacct
     6541 ccgcgggcgg cacctcgccg gagtcggcag gacagccctt cacgcagtcg cttgccttca
     6601 gcgctgacgg cggcaaaacg tgggagaaat tcgccggcaa cccggtgctg ggccacatcg
     6661 tggaaagcaa ccgtgatccc cgcatcctct ggcacgaacc aacggaatcg tgggcgatgg
     6721 cgctctatct cgacaagaac gactacgcgc tgttcacctc gccgaacctg aaagactgga
     6781 cacggacgtg cgacgtcagc atccccgata cctccgagtg ccccgacttc ttccccatgg
     6841 cacaggacga cgacccggcg cacgtgaagt gggtgttctg gggcgcgaac ggctgttatc
     6901 tcctcggcgc gttcgatgga aaaacgttcg tgccggagga aggcccctgt cgcctcgact
     6961 caggggccaa tttctacgcc gcgcagacgt ggagcgacat ccctgaggca gatggtcgac
     7021 gcatccagat cgcgtggatg aacggcggcc agtaccccgg catgcccttc aaccagcaga
     7081 tgagcttccc ctgcgccctt tccctgcgca gcacgtcggc cggcgttcgc ctctgccaga
     7141 tgccggtccg tgaaatcaaa tgcctgcggg aggacagcgt catccgcacg gacgtcgcgc
     7201 tcctccccgg aaggttgctc gatgcgtcgg cgggcgaact cctcgacatc gaagccgaga
     7261 tcgaaccctg gtgcgcgaag gcgatcgagt ggagcatccg cggcaacgca attcggtacg
     7321 atgcggagaa gcacctgctt tcctgctgcg gcagggaagc tcccgttgac cttgcgggcg
     7381 gccttctgcg gctgcgcatc ctcctcgacc gcaccagcct cgaactgttc tccggcgacg
     7441 gcaatgtcgg gtttcatcaa tgcttccttc ccgacgcggc gaacaccggc gtcgagctgg
     7501 ccgcgacggg cggtgaagcc cgcatacgat cgctcgccgt gcaccgactc cgctctgcat
     7561 ggtgatacaa aaaactggag ggacccactg agaacgccgt ccgttcttcg tcgggtaccc
     7621 cgccacctgt tctccccccc tcccgacaac ccgtcatcct ggcctgacaa cctgtcatcc
     7681 tgagcggagc gaaggatctc ttttcggcgc cctcgttcnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnncc gcaccgcccg ggacgttgcc ccgggctgaa ttatctcggc
     7861 ccttcaggcc ttacccgccc cacttgatcc agccaaagcg ccagcggcgc gaaatacccc
     7921 tgcgggcgcg cgtcccgtat gcccctccgt cccactttgc ccaaacacct atccaccccc
     7981 tccttcccgg gtcgtgtgtg gcgtacgttt cagattgcac acaccggtgt ccacccaccg
     8041 catgttccca aacctcgggg gcaaccatga catccgccgc caacacggca caagtcaccc
     8101 gctcaatcaa ttgcttccgc atcggcacgc ctcagtggct ctccgacgaa cggttcgcgg
     8161 ccctgctcgc acttttcgac cgctaccccg gtgtgactga cgaagtcacg tttttcactt
     8221 cggaaacgca cccgccactc ccccttgagg tgctccgtga ccgctgcgtg ctcctttcgc
     8281 gccgaatgga cgaagtgcgc cggcacggct accagaccgg catcaacatc cttgccacca
     8341 tcggccacca cgaggaaaac ctgcccaact ccctcgacgg cccgtacacg cggatgactg
     8401 atccggacgg acgcatttca cgcggcgtgc tctgcccgaa cgacgagcag gcgcgggagt
     8461 atatccgcga agcgtaccgt ctcatcactg aagcgaatcc cgactacatc tgggtggacg
     8521 atgatgtccg cctgttcggg cacgggccca ttcttgccgg gtgcttctgc gacacctgcc
     8581 tcgcggcgtt ctcccgcgaa acgcggcggg aatggtcgcg cgagtccctc cgtgaggcgg
     8641 tgaacagcgg gccactggcg gaccggctga tgtaccgccg cgcgtggctt gcccacaacc
     8701 gggccactat cgcccgcctt ttttccctga tcgaacagac ggtgcatgcc atccgccccg
     8761 gattgcccat cgggttcatg acgggcgagc gctatttcga ggggtacgac ttcgacgcgt
     8821 gggcggaggt gctttccggt gcttcggcgg cacccgttcg gtggcgaccg ggcggcggaa
     8881 catacaccga cgaaaaactc gacaacatcg tcggcaaggc gcacgacatt gggcgccagg
     8941 tcgcttttct gccctccgcc gtaacggtga ttcagtccga aatcgagaat ttcccgtacc
     9001 aacgcctgaa gaaaagcgcg aaggcgaccg cgctggaagc ggccgcgtat atcgccgccg
     9061 ggtgcacggg ggcggcgtac aacgtgctct ccatgtacga cgagccgctc gacgaatacg
     9121 agccccttgt gcgcgtcctg accgaaaatc ggccgttcct cgacgcgctt gccaaagcgg
     9181 gcgggcgctc gtcgcccgtc ggcctgcaca gcggctggtc aaaggacagc ttcgcgacgt
     9241 tacagcttgc cgagggcgac tggctcttcc cgcgggaagg cgttcccgga gggcagacga
     9301 acgagtttca cgaaatcggc atcccccccg cgtattcgct tgacgcagcc caggcggtgg
     9361 cgttgacggg aaacggcgtg cgggcgtttg acagaagaac gcttgagcgc atcctttcag
     9421 gcggcgtcta cctcgacgcc gaagcagcca gagccctcga agagatgggg catgccgaac
     9481 tcgtcggttt ccgtgtgggc gaggcgtttc cgattgacgc cattgagcaa ttcctcccca
     9541 atccgctctg tggcgccttt gccggacggg agcgggacgg ccggcagtcc ttctggaaac
     9601 gcaccgccta cgccctcgaa ccgatgcccg gcgcggagcc gctcgcccgc cttgtggact
     9661 atggccggaa ggagctcgcc gcgtgcgggc tcggggtgtt tgagaactcc ctcggcggcc
     9721 gtgtctgcgt cgccggatac gcaccgtgga cgtttctgca gaacctcagc acgtcatcgc
     9781 gcacgaaggc aatcgtccgc tggctctcgc gggaaactct gcccgcgtat gtcgagtcct
     9841 tccaccggat agtgctgtgg gtgcgggagc ctgagcccgg caaggtcgcc attgccctcc
     9901 tcaacgcatc gctcgacccg gcacggcagg cctcgctcct cgtccggact gccgcgaatg
     9961 aactgcgcct cacggacatg cgcatgcagc aggccactgt
//
LOCUS       DAOTNG010000084         9961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6301_length_9961_cov_4.438926, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000084 DAOTNG010000000
VERSION     DAOTNG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_9961_cov_4.438926"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..418
                     /locus_tag="P5179_05595"
     CDS             92..418
                     /locus_tag="P5179_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRS94725.1"
                     /translation="MPAGDGTGPLGMGPMTGRRAGYCAGFGVPGYANPGAGFWCGGGR
                     GRGFRNRYWATGIPGWAWWPGVSGTVPAPVTEEAEAEALRQQAAALQRSLDAINQRLA
                     NLTSEK"
     gene            complement(511..1641)
                     /locus_tag="P5179_05600"
     CDS             complement(511..1641)
                     /locus_tag="P5179_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94726.1"
                     /translation="MYRKPDYATARNPKKFSWSASGSYLSCLALSGVPLRDFFLKPDA
                     CIEAYRVGREKQRQLFDPQDVSLPSPATPPVSYGHANGLGARLFFPEGGEVAVEHCFA
                     HSLEEAMEALARPVEFASAGMAPFYLDFREQMMRAFPGESVGFSFGAEGPCTTAYELR
                     GDAFFVDVMEEPARTKEFLSRINDSILAFEQFRLGVSGGKVFNESGASLCDDIAAMIP
                     PRLWNEFVVPFWRDLFCRRTTGDVHAHVEDLKPAHLPFLEQIGLSYYDPSISHHLNPR
                     IIADKCRVPFGWRLGGIHLPVLDAQDVEDFVFQSAADGASRVFTILEANSCSGENIPK
                     IDAFIRAAKEVKRLLDEGCPRRALADHVSERGRHKFWDNWCS"
     gene            1889..>3786
                     /locus_tag="P5179_05605"
     CDS             1889..>3786
                     /locus_tag="P5179_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS94727.1"
                     /translation="MSCPQLPTRVKNVLVALAAAFSFSPAFAAGIEQIWVADAHMVME
                     GAPMVVDLDGDGDAEILTAAYEHIIVMDGSGSELWRYDGRGRYSTCPAILERRGDTLL
                     IFAGDNTGEFTCLDGRGNVVWKRDLPSIFCASPAVADLDADGTFEVVQGDKTGKIHAM
                     DALSGALLWTAQLDGECASPAVGDLNSDGKLEICITTGAGKVYAVSATGVVLWSVDLG
                     SPSPDWSTCSPVIFTGSDGNQYVAAGARSGRFVCLNARGELGWERDLRGAIASTISVS
                     DFDSDGIADIFVVTQLGMLYRFDETGRVIWDIDTQGRSLAAGAIVDVDGDYALEYVLC
                     TQNGNLLVFNQTGEITFNHQFPNRTINVTAAFGDIVKTRPGLEFAITGGESGKVYCFG
                     TAAPVNTAAPWRTYRRDNELTGCWSGLRSDTGARMTPANLAWDRILTGEDVVFHIFLP
                     GPVGLAKAEATCIRPDGSRQSSIGAVAGQRGVLQMPVVITAPGVYRFEWNVKDGAGRR
                     LAGGSRELTLHPYQNDRAFVRRTLNELRSALGTTPVPRSERGIRAALQKEMRDIEREA
                     EVLAALQDAVPGAARELVEEANARTVALNERARRASALARVARALPAGTSSPRVVAFE
                     GTTWENRD"
     assembly_gap    3787..3886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3887..5397
                     /locus_tag="P5179_05610"
     CDS             <3887..5397
                     /locus_tag="P5179_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94728.1"
                     /translation="SSPRVVAFEGTTWENRDINRQLPAAVAIPLTISRRAVTGEHEPV
                     SIKLLNVTLDTVRAEVRVETSNAGIRVTPMEAKAVPTNLNTKGWDALPGLGNRAIAVP
                     PLETGEVWLDIDLANTPPGKHTVTAFFGRGSAAARAIITLDVLPFEMAGYRDLRLCNW
                     AVYDGDAVPDLLAHGNTVFITGMPHATISPENPDRVSVDFARLDAFVEPLRGHDVFLL
                     LSGVPNLGVPVSSDTFVVRFGDYIRQVLGRLAQRGIPERNVALYPYDEPGGSGWNTVN
                     RYIEFARQALKAYPGLLFYVNGGGDLPMFQAFNEVAGIWCPGYYMLPDSSAEMNFIRA
                     SGKTLWSYDCGIGYARPIGWHTKTINVAGQYRMAPVFTVAFGATGLGYWCYNHGPSMW
                     EAVEAEFPLVYVNPDTTHTASRRWEAVREGMEDARIMLALRERLSDEHVNAAAKQKIR
                     HLLEVTAPDFARHSLEEVRLGVARYALDATNNDGTVGAFRQELLDCVAATVE"
     gene            5531..7981
                     /locus_tag="P5179_05615"
     CDS             5531..7981
                     /locus_tag="P5179_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRS94729.1"
                     /translation="MAQGSTTRTTLPISGMSCAACAARIERKLRALDGVADAEVNLAA
                     HTALVTHDPGRAPAPRIVDAVREAGYDVPVETLRLPVEGMSCAACVSRVERALKGVEG
                     VLDAQVNLATGEATVRAVLKDDSGTRAIATAVEKAGYGVRSVEYSRQPSADPVARSEE
                     EFKNLRRRFAIAAVFAIPVFLISMGLVPGMARLPFQTTAILLLALTLPVQFWAGWQFH
                     RGAWQQLRHASADMNTLISVGTNAAFLFSLVLTFHPAAFRSTGVHHAVYYETSAVIIT
                     LVLFGRMLEARAKARTTQAMSALLALRPVAAHVERDGQIRDVDPADVVAGDVLVVRPG
                     ERLPVDGVVLDGFSSVDESMLTGESLPVEKGKDDTVAAGTLNVAGSFRYRATRVGNET
                     TLAQIIRLVEHAQGSKAPVQRLVDRISAVFVPTVIAISAVTFVVWWAFGPEPALHFAV
                     LNAVAVLIVTCPCALGLATPTAIMVGTGRGAEMGIFIRSGETLERVGRLTTVVFDKTG
                     TLTQGEPVVTGVHTVGTLPADDVLVLAASAEARSEHPLGAAVVRKAAERSLPLLSCEE
                     FSAESGHGLRAMVANRVVLIGNSALMQAQGVRLDAGRSVAEEIQAKGATAVFVALDGA
                     LEAVFEIADTPKPHAAEVIAALKRDGLKVVILTGDSRRTAEAIASQLGADDVIAEVKP
                     GAKADYVAALQKSGEVVAMVGDGVNDAPALALADIGIALGTGSDAAMETAGITLMGGD
                     IRNVRKAIALSRATLRTIRQNLFWAFIYNVAGIPIAAGVLYPVNGMLLDPMLASAAMA
                     MSSVSVVTNSLRLRRWNG"
     gene            complement(8036..8809)
                     /locus_tag="P5179_05620"
     CDS             complement(8036..8809)
                     /locus_tag="P5179_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRS94730.1"
                     /translation="MIGALAGDIIGSVYEWDNIKTKDFPLFSPRCTFTDDTVLTVALA
                     DSILSGVPFARNMKTFYRLYPDRGYGSSFHTWARSTDMSPYQSWGNGAAMRISPVGYA
                     YDDLEQVLRKAKEFTEVTHNHPEGIKGAQATAASILLARKGKTKDEIRSFVESEFGYD
                     LSAHVDEIRPTYTFDESSQGTVPQAIRAWFDSTDFEDAIRTAVSLGGDSDTVACITGG
                     IAQAGYGGVPEHISARVYETLDERLRAITQQFMMRFCGG"
     gene            complement(8806..9540)
                     /locus_tag="P5179_05625"
     CDS             complement(8806..9540)
                     /locus_tag="P5179_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94731.1"
                     /translation="MSLTAAFAAVLMSAPPAHAQYTVRAGAAAGGTVGEWGRVAGFLF
                     GVDQMTPSRSFGWWSTHFNVERLSTRFERTEKLAGLTLIRTYASPPETTAFKNSFSVT
                     AITLGARYEPCRRWLGFRQTSKHLLSPFVGWYGGYMRMEHTGSREYHVAWTSVSSHGV
                     PVGAVAGYFMGWGGPPENALDPGVSSTYTGLEIGLDVSSTAWISGHTFVAPRAGLPDG
                     LKTPYAPRVLLRVSIAHRTAPVRRDG"
     gene            complement(9570..>9961)
                     /locus_tag="P5179_05630"
     CDS             complement(9570..>9961)
                     /locus_tag="P5179_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRS94732.1"
                     /translation="AGALMVFVIARILHWVLRRIGGVGSWTATRTAVAWALAPGVPSL
                     ALWAIMVTGYGYSALTPSLISGSDDPRALVLTVDYGVQFGLTVWSLLIEILCLSDAHR
                     LAVWKVIAAEAGLGLAIGLLTLIGFNL"
BASE COUNT         1874 a         2866 c         3295 g         1826 t
ORIGIN      
        1 gggggttttg attcaaaatc gcgctccaga tgatggtcag catcatgagg gcgcacgtac
       61 atcaacacac ttacgataag gaggcacagc aatgccggca ggagatggca ctggtcccct
      121 gggaatggga ccgatgacgg gtcggcgcgc gggatattgc gccggattcg gcgttccggg
      181 atacgcgaac cctggagcgg gtttctggtg cggcggtggc cgcgggcggg gattccgcaa
      241 tcggtactgg gcaacaggaa tacccggatg ggcgtggtgg ccgggcgtat cgggaaccgt
      301 ccctgcgccc gtgaccgaag aggccgaagc ggaagcactg cgtcagcagg cagcagctct
      361 tcagaggtcg ctggatgcga tcaaccagag gcttgcgaac ctgacgtcag agaagtaaac
      421 tgccgatacc gtccgcccct gtggagccgg gatgctctca aggggcagag cagcggggga
      481 gtccacgagg actctcccgc ttttcgtgtg tcaggagcac cagttatccc agaatttgtg
      541 tctgccgcgt tcgctgacgt gatcggcgag ggctcttcgc gggcatccct cgtcgaggag
      601 acgcttcact tctttcgccg cgcggatgaa cgcgtcgatt ttcggtatgt tttccccgct
      661 gcacgaattg gcttccagaa tcgtgaacac tctggaggcc ccgtccgcgg ccgattgaaa
      721 gacaaaatcc tccacatcct gcgcgtccag aaccggtaga tgaattcccc cgaggcgcca
      781 gccgaatggt acgcggcact tatccgcgat gatacggggg ttcaggtggt gggagatgga
      841 cgggtcgtag tacgacagcc cgatctgctc caggaaaggc aggtgagcgg gtttcaggtc
      901 ttccacgtgc gcatggacat cacccgtcgt ccggcggcag aagagatccc tccagaacgg
      961 cacgacgaat tcgttccaga gccgcggggg aatcattgcg gcgatgtcat cgcacaggct
     1021 tgcgccgctc tcgttgaaca cctttccgcc ggagacgccg aggcgaaatt gctcgaacgc
     1081 gaggatgctg tcgttgatcc ggctcaagaa ctccttcgtc cgcgccggtt cctccatcac
     1141 gtccacgaaa aatgcatcac cccgcaattc gtaggctgtc gtgcagggac cttccgcccc
     1201 gaaagaaaag ccgaccgact caccgggaaa cgctcgcatc atctgttccc ggaagtccag
     1261 gtagaacggg gccatccctg cgctggcgaa ttcgacaggc cgcgcgagag cttccatcgc
     1321 ttcttccagc gagtgcgcga agcagtgctc gaccgcgacc tcgcctcctt cggggaaaaa
     1381 caaccgcgcg ccgaggccat tggcgtggcc gtaacttacc ggcggcgtgg cgggagacgg
     1441 cagggaaaca tcctgcgggt cgaaaagctg cctctgtttt tcgcgcccga cccgatacgc
     1501 ctcgatgcag gcatcgggtt tgagaaagaa atcgcggaga gggaccccgg acagcgcaag
     1561 gcaggagaga taactgcctg acgcgctcca cgagaacttc ttcggattgc gcgcggttgc
     1621 gtagtcaggt ttgcggtaca tggtggcctc ctgataccaa taggtcagaa aaagccgaca
     1681 cgggaccaag ctggcgtgag aaccggagtg cgacgaggga acttggtgcg gaggccaccc
     1741 ggcactattg tacctagcga gtccgggagt ctggctgtcc cggcgtcagt gattgtgttc
     1801 ccacaggttg catcctcctg aacgccctca tgcagggcgg cgagcgtctt cctcaaggtt
     1861 ttttgactcc gcggaaagga atctcagcat gagttgccca caactaccca cacgggtaaa
     1921 aaacgttctg gtggctctgg cagccgcttt ttctttttcg ccggctttcg cggccggtat
     1981 cgagcagatc tgggtggccg acgcccacat ggtgatggaa ggggcgccaa tggtggttga
     2041 cctggatgga gatggagacg cggaaatcct gactgcggcg tacgaacaca tcatcgtaat
     2101 ggatggttcc ggctcggagc tctggcgcta cgacgggcgg ggacgatatt cgacgtgtcc
     2161 cgcgattctc gagcgccggg gagacaccct gctcatcttc gccggggaca acacgggtga
     2221 attcacgtgc ctcgacggcc gtggcaacgt ggtgtggaag agggacctgc cttcgatctt
     2281 ctgcgcgtcg ccagccgtgg cggatctgga tgcagacggc acgttcgagg tggttcaggg
     2341 cgacaaaacc ggcaaaatcc acgcgatgga tgccctgtcg ggagctcttc tgtggacggc
     2401 acaacttgac ggggagtgtg ccagcccggc ggtcggcgat ctgaattccg acgggaaact
     2461 ggaaatatgc atcaccaccg gagccgggaa ggtatacgcg gtgagcgcca ccggtgttgt
     2521 gctctggagc gttgatcttg gttcgccctc gccggactgg tcaacctgtt ccccggtgat
     2581 tttcacgggg tccgacggga accagtacgt ggcggccggt gcgcgctcgg ggcgctttgt
     2641 ctgcctcaac gcgcgtggtg agctgggctg ggaacgggac ctgaggggcg ccatcgcctc
     2701 aacgatttcg gtgagcgact tcgattccga cggaatcgcc gacatcttcg tcgtcaccca
     2761 gcttggcatg ctgtaccgtt ttgacgaaac gggcagagtg atctgggata ttgacacgca
     2821 ggggagaagc cttgctgccg gagcgattgt tgacgtggac ggggattatg cccttgagta
     2881 cgtgctgtgc actcagaacg gcaatctgct ggtgttcaac cagaccggag agatcacgtt
     2941 caaccatcag tttccgaacc gcaccatcaa tgttacggcg gcgttcggcg acatcgtgaa
     3001 aacccggccc ggtctggaat tcgccatcac cggcggcgaa tcgggaaagg tgtactgctt
     3061 tggcacggcc gcgccggtga acaccgccgc gccgtggcgc acgtaccgga gggacaacga
     3121 gctgacgggc tgctggtccg gcctcaggtc cgataccggg gcacgcatga cgccggccaa
     3181 tctcgcatgg gaccggatcc tgaccggaga ggatgtcgtg ttccacattt tcctgcccgg
     3241 tcctgtgggc cttgcgaagg cggaggcaac atgcatcagg ccggacgggt ctcgccagtc
     3301 ttcgataggc gcggtagccg gccaacgagg cgtgcttcaa atgccggtgg tgattactgc
     3361 accgggggtg tatcggtttg agtggaacgt gaaggatggc gctggaagac ggctcgccgg
     3421 tggctcgcgg gagctgacgt tgcaccctta tcagaatgat cgtgcctttg tccgacgcac
     3481 gctgaacgaa ctgcgttccg cactgggaac cacgcccgta ccgcgatcag aacgcggcat
     3541 ccgcgccgcg ctgcagaagg agatgcggga tattgaacgc gaggccgagg tactcgcggc
     3601 gcttcaggat gcagtcccag gagcggcgcg ggagctcgtt gaggaggcaa atgcgcggac
     3661 ggttgcactg aacgagcgag ccagacgggc ttcagcgctc gcgcgggttg cacgcgcgct
     3721 gccggcgggc acctcctcgc cgcgggtggt cgcgtttgaa gggaccacgt gggagaaccg
     3781 ggacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctc ctcgccgcgg
     3901 gtggtcgcgt ttgaagggac cacgtgggag aaccgggaca tcaaccggca gctaccggcg
     3961 gcagttgcga tcccgttgac gatttcccgt cgggcggtaa ccggggaaca tgaaccggtg
     4021 tctatcaagc tgctcaatgt aacactcgac acggtgcggg ctgaggtgcg cgtggagacg
     4081 agcaatgccg gcattcgagt taccccgatg gaagcgaagg cggttcccac gaacctgaac
     4141 acgaaaggct gggatgccct gcccggactc ggaaatcgcg caatcgcagt tccgcctctg
     4201 gagacaggtg aagtgtggct ggatattgat ctggccaaca cgccgccggg caagcacacg
     4261 gtaacagcgt ttttcggaag aggcagcgcg gccgcccgcg ccatcatcac gctggatgtg
     4321 cttccgtttg agatggcggg gtaccgcgat ctacgcctgt gcaactgggc ggtatacgat
     4381 ggcgatgcgg ttccggacct gcttgctcac gggaataccg tattcatcac ggggatgcct
     4441 cacgccacca tcagccccga gaacccggac cgcgtatccg ttgatttcgc ccggctcgat
     4501 gcgttcgttg aaccgctacg ggggcacgac gtgttcctgc tgctgagcgg cgtcccgaac
     4561 ctcggtgttc cggtgagcag cgacacgttt gttgttcgat ttggcgacta catcagacag
     4621 gtgctcggtc gccttgcaca gcgcgggata ccggagagga atgtggcgct gtacccctac
     4681 gacgagcccg gaggcagcgg ctggaacacg gtcaaccgct acatcgagtt cgcgcggcag
     4741 gccctgaagg cgtatccggg gcttctgttc tacgtgaacg gcgggggcga tcttcccatg
     4801 ttccaggcat tcaacgaagt ggcgggaatc tggtgccccg gctactacat gctgccggac
     4861 agctccgccg agatgaactt cattcgcgcg agcgggaaaa cgctgtggtc gtacgactgc
     4921 ggaatcggat acgcgcggcc aatcggctgg cacaccaaga cgatcaacgt tgccggacag
     4981 tatcgcatgg cgccggtgtt caccgttgcg ttcggcgcca cggggctggg gtactggtgt
     5041 tacaaccacg ggccttccat gtgggaggcg gtggaagcgg agtttccgct ggtgtacgtc
     5101 aaccctgaca ccactcacac tgcttcgcgg aggtgggaag cagttcgcga agggatggag
     5161 gatgcccgca tcatgctggc gctgcgggaa aggctttccg atgagcacgt gaatgctgcg
     5221 gcgaagcaga aaatccgtca cctgctcgaa gtgacggcac cggattttgc ccgccattca
     5281 ctggaagagg ttcggctggg cgtggcgcga tacgctctcg atgcaacaaa caatgacggt
     5341 acggtgggcg catttcggca ggaattgctg gactgcgttg ccgcaacagt cgagtaagcc
     5401 gcaccggttc ggggcgcaag aggccggcag gggcgtttcg cctcctgccg gtttcctttt
     5461 gttctgcgcg gagtacgttc gtccttctcc tgtgttgttc ttcagtgaac cagtgtgaaa
     5521 gggaaccgta atggcacagg gatccaccac ccggaccaca ttaccgattt cgggcatgag
     5581 ctgcgctgcg tgcgcagcgc gcattgaacg gaagttgcga gcgttggacg gcgttgcgga
     5641 cgcggaggtg aatctcgcag cgcacacggc gttggtcacc cacgaccccg gaagggcacc
     5701 ggcgccgcgg atcgttgacg ccgttcgcga ggccgggtat gacgtgccgg tggagacatt
     5761 gcgcctcccc gtggagggga tgagctgcgc tgcgtgcgtg tcccgcgtgg agcgcgcact
     5821 gaaaggcgtg gaaggcgtgc tggacgccca ggtgaacctt gcgacaggcg aggccaccgt
     5881 ccgtgccgtg ttgaaggatg attctggtac ccgcgcgatt gccacggcgg tggaaaaggc
     5941 cgggtacggc gtcaggtcgg tggagtattc gcgtcagccg tccgcggatc ccgtggcgcg
     6001 ttccgaggag gaattcaaaa atctgaggcg acggtttgcg attgccgcgg tatttgcgat
     6061 ccctgtgttt ctgatcagca tgggcctggt gccgggtatg gcgcgtctgc ccttccagac
     6121 caccgcaatt cttctcctcg cgctcacctt gccggttcaa ttctgggcgg gatggcagtt
     6181 ccaccggggc gcgtggcagc agttgcgcca tgcgtccgcc gacatgaaca cgctgatttc
     6241 ggtggggaca aacgcagcgt tccttttcag cctggttttg acgttccatc cggccgcgtt
     6301 ccggagcacc ggggtacacc acgcggtcta ttacgaaacg tccgccgtca tcatcacgct
     6361 ggttcttttc gggcggatgc tcgaagcgcg cgcaaaagcc cgaacgacgc aggccatgag
     6421 cgcgctcctg gcgctgcgac cggtagctgc tcatgtggag cgggacgggc agatacggga
     6481 tgttgacccg gcggatgtgg tggcaggcga cgttcttgtc gtgcgcccgg gcgaacgcct
     6541 gcctgtcgat ggagtagtac tggacggttt ttcgtcggtg gacgagagca tgttgacggg
     6601 tgagagcctc ccggttgaaa agggaaagga cgataccgtg gccgccggga cgctgaatgt
     6661 cgcgggaagt ttccgatacc gagccacgcg ggtggggaac gagacgacgc tggcccagat
     6721 catccggctg gtggagcacg cgcagggttc caaggcaccc gtgcaacggc tggttgaccg
     6781 gatatccgcg gtattcgttc cgacggtgat cgcgatttcg gcggtgacgt ttgttgtgtg
     6841 gtgggctttc gggccggagc ctgcgctcca ttttgcggtg ctgaacgccg tcgcggtgct
     6901 gatcgtcacg tgcccctgtg cgcttggcct tgccacaccg acggcaatca tggtgggaac
     6961 ggggcgaggg gcagagatgg gcatcttcat cagaagtggc gaaacgctgg aaagggttgg
     7021 caggctgacg acggttgtgt tcgacaagac gggaacgctg acacagggag aacctgttgt
     7081 gacgggggta cacacagtgg gtaccctgcc agcggacgac gtgctggttc ttgcggcgtc
     7141 ggcggaggcc cggtcggagc accctctggg ggccgccgtt gtcaggaaag cagcagagcg
     7201 ttcgcttccg cttctttcgt gcgaggagtt ttcggctgag tcgggccacg ggctgcgggc
     7261 aatggttgcc aaccgggtgg ttctgatcgg caattccgcc ctgatgcagg cgcagggcgt
     7321 gcgcctggat gcgggaagga gcgttgcgga ggagattcag gccaagggag ccaccgcggt
     7381 gttcgtggcg ctcgatggcg cgctggaggc ggttttcgag attgccgata cgccgaaacc
     7441 gcacgccgcc gaggttatcg ccgcgctgaa acgcgacggc ctgaaggtag tcatcctgac
     7501 aggggattcg cgacggacgg ctgaagcaat tgcatcacaa ctcggcgcgg atgacgtgat
     7561 cgcggaggtg aaaccgggtg cgaaagcgga ttatgtcgcc gccctgcaaa agagcgggga
     7621 ggtcgttgcg atggtgggtg acggcgtaaa cgacgcgccc gcgctcgccc tggcggacat
     7681 cgggatcgca cttggcacag gaagcgatgc cgcgatggag accgcaggga tcaccctcat
     7741 ggggggcgac atacgcaacg tgcggaaagc gatcgcgctt tcccgtgcga ctctgcggac
     7801 gatacggcag aacctcttct gggcattcat ctacaacgtg gcgggcattc ccatcgcggc
     7861 gggcgttctt tacccggtga acggcatgtt gctcgacccg atgctggcaa gcgccgcgat
     7921 ggcgatgagc agcgtgagcg tggtgacgaa cagcctcagg ttgaggcggt ggaacgggtg
     7981 agctttctga acgcaccccg tgtgcggcgg aaccgggctc acggtgcgag ggaaatcagc
     8041 ccccgcagaa gcgcatcatg aactgctgcg ttatcgcacg cagacgttcg tccagggtct
     8101 cgtacacccg cgcggaaatg tgttcgggta ccccaccgta tccggcctgc gcgattccac
     8161 ccgtaataca cgcaacggtg tctgagtctc ccccaagaga aacggcagtg cggatcgcgt
     8221 cctcgaaatc tgtggagtcg aaccacgcac ggatcgcttg agggaccgtt ccctgcgagg
     8281 actcatcaaa cgtgtacgtc ggccggatct catcaacgtg agcgctcagg tcgtacccga
     8341 actccgactc gacaaaggac cgtatctcat ccttggtttt gcccttacgc gccagcagta
     8401 tggaagccgc ggttgcctgc gcccctttta tcccttcggg atggttgtgg gttacttcgg
     8461 taaactcctt cgccttccga agcacctgct caaggtcgtc gtacgcgtac cccacggggc
     8521 ttatgcgcat ggcagcacca ttcccccagc tttggtacgg ggacatatcc gtgctccgag
     8581 cccatgtgtg gaacgacgag ccgtatcccc ggtcgggata aagccggtag aacgttttca
     8641 tgttacgggc aaagggcacc ccggagagaa tggagtccgc gagggcaacg gtaagaacag
     8701 tatcgtcggt gaacgtgcat cgcggtgaaa acagaggaaa atccttggtc ttgatgttat
     8761 cccattcgta caccgaaccg atgatgtccc ctgcaagtgc tccgatcatc cgtccctccg
     8821 aacaggcgcc gtgcgatgcg ctatggaaac acggaggagc actcgcgggg cgtacggcgt
     8881 tttgaggccg tcgggcaggc cggcgcgggg cgccacaaag gtgtggccgc tgatccacgc
     8941 ggtgctgctt acgtcgaggc ctatctcaag gcccgtgtag gtgcttgaga cacccggatc
     9001 cagcgcattt tcgggcggac ctccccaccc catgaagtac cctgcaaccg cccccacggg
     9061 tacgccgtgc gaggagaccg aagtccacgc gacgtggtat tcgcggcttc ccgtgtgctc
     9121 cattcgcatg taccccccgt accagccgac gaacggggac aggaggtgtt tcgaggtctg
     9181 ccggaaaccg agccatcgcc ggcagggctc atatcgcgcc cccagagtaa tcgcggttac
     9241 ggagaagctg ttcttgaagg ccgtcgtttc ggggggtgag gcgtaggttc tgatgagcgt
     9301 caatcccgcc agcttttccg ttcgttcgaa ccgcgtggat aaccgctcga cgttgaaatg
     9361 cgtcgaccac cacccgaagc tccgcgacgg tgtcatctgg tccacaccga agaggaaccc
     9421 cgcgacgcgc ccccactcgc ccacggtccc gccggcggcg gcacctgcgc ggacggtgta
     9481 ctgcgcgtgc gccggggggg ccgacatgag gacggccgcg aaggccgccg tgaggctcat
     9541 cagcgcacga agctttgagg agaaacctgt cacaggttga aaccgatcaa ggtaagtaaa
     9601 ccgatagcca gaccgagccc tgcctcggcg gcgatcactt tccacactgc cagccggtgc
     9661 gcatcggaga ggcacaggat ttcgatcagc agagaccaga cggtgagccc gaactgaacg
     9721 ccgtaatcaa cggtgagtac gagggcccgg gggtcgtccg atccggagat gagcgacgga
     9781 gtcagggctg agtagccgta accggtcacc atgatcgccc agagtgccag acttggaacg
     9841 cccggggcca gagcccacgc gaccgccgtc cgtgtcgccg tccacgaccc cacaccgccg
     9901 atccgccgaa gcacccagtg caaaatccgg gcaatgacaa agaccatgag cgcaccggcc
     9961 a
//
LOCUS       DAOTNG010000085         9861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6371_length_9861_cov_5.828676, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000085 DAOTNG010000000
VERSION     DAOTNG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6371_length_9861_cov_5.828676"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="P5179_05635"
     CDS             <1..1423
                     /locus_tag="P5179_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94733.1"
                     /translation="EWRFRDWVIPTFRRLVSERLIPDRHNVAKVTPVAYHLPRCERPL
                     EFHAVSDDLDFDHGAGRLIRGTFGVYDRARDPELIPNDPRFGWFPVLPVKTDRSVLTQ
                     FKRVLRPGDVNSPEHARELMGKYYPPVDRGTAWSQIVGDLVFAANTHENWFVPEEVRL
                     QVPRRPANVRIESTGTAQLRLRWEKAEGDNAYRVWRIRDGVETCLTPNSIEETEYLLP
                     PVEQGDHFAVSAITSATEEFAQTLHLHQFLVLNRAESRRSEWVNAAGDRMERFRFAES
                     VPPVSEHVLEAERRCAGCLPVQDLTSPPVTPDDPFASVKLAIMDHMAQWKSFVEAEDL
                     DGIMSMYAADYSEPDGRGKDWVEAAFRLVLRRYMESQFARLVKEWGALPGWRNPAFRL
                     LVREWRSVSNDAAEVLVVAHMWAGGGPEMEPSDMFNHPFGRPHTTTMRWKLISGEWKI
                     TSTDPAFLRIEDTAIFRFRYQGW"
     gene            complement(1485..2264)
                     /locus_tag="P5179_05640"
     CDS             complement(1485..2264)
                     /locus_tag="P5179_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS94734.1"
                     /translation="MGNLKVGVMIESFRLGVRNGIKKAAEIGADGFQIYVTGGEMHPD
                     NLSKTGRQEFLKFVADQGLVISALCGDFGKPYTKEEGLDELITDTKKVIDLAVDLKTP
                     IITTHIGVVDADPKARSRQIMADALNNVGAYADNRGIMFATETGPEPGPVLADFIKSL
                     KTQAVKANFDPANLAMMGFDVLEGVRALGPYIVHTHAKDGIREDGRGKEVPLGEGAVP
                     WEEYIDLMTAQGYRGFYTIEREVGDDPAADVKKALDFLRKW"
     gene            complement(2320..3696)
                     /locus_tag="P5179_05645"
     CDS             complement(2320..3696)
                     /locus_tag="P5179_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS94735.1"
                     /translation="MATNVIVVFGDEMRGQAMGCAGDPNVSTPCLDSMASRGCRFPRA
                     YSNTPVCTPARGTMLTGLWPTAHGAVVNDIAIRTDVTSIAHILNSHGYRCGYIGKWHL
                     GGIPRYKFTPPGPERLGFDAFWAVWNCHHDYMNPRYYLDTPEPVVMEGYEPSVQTDLA
                     MAFMDRHREQDNEHPFCLFLSWGPPHSPYEPLPPGRETQYDPSALVLPPNCPDTPENR
                     RHLAGYYAHISALDAETQRLLDYLRANNLEKDTLVVFTSDHGSMLGSQGHTHKQRPWA
                     ESVVVPLIFYRPGAVPEGRVNKTLFSLLDLVPTLLGCLDLQVPPSMHGEDLSACVRSG
                     ATEEGRVLYLADLITTDQANHYGIKPWRGIKTERYTYACDTQGEWLLYDDERDPYQLE
                     NLAGDPLATETRASLHQELQRQMERFGDRIMDVAEALEAWGLTEAFRIRTDHLYASPH
                     NMGGRLPQ"
     gene            complement(3712..4818)
                     /locus_tag="P5179_05650"
     CDS             complement(3712..4818)
                     /locus_tag="P5179_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRS94736.1"
                     /translation="MDERPGADFSSVAIVGVGLIGGSLALALKEFGATGRVVGISSRK
                     TIKEALGLGAIDEGWEYNDLALALRETKAVFLCTPVSRIIELLPEVAHVITPGTVVSD
                     VGSTKKEIVERAEKCMPQHSVFIGGHPMAGSERNGVGAADPFLFQNALYVLTPSGSVP
                     ESVRASFTERIASIGARVFCLSASAHDRVAAAVSHLPQMIALALVEYVGTLNAIEPAH
                     LQMAAGGFRDMTRIASSPYSVWQDILATNASEIRKALRGFAGQLEQVLADIEHMEPHF
                     RAANEIRSSIPRDTKGFLSPLCDVLVRCEDRPGQLARMTAALAREQINIMDIELLKIR
                     EGEGGTFRMAFKDIQTADAAIRTLDKEGFPARLR"
     gene            complement(4821..5909)
                     /locus_tag="P5179_05655"
     CDS             complement(4821..5909)
                     /locus_tag="P5179_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94737.1"
                     /translation="MEFRCKDDWEKARTRIEAWWNNEIIDRAVVAVTAHNGRPRKEIP
                     TPASMVERWTNADYLIARQKAHAETTYYGGESFPLMFVNLGPSVGSAYLGCELAFAET
                     TAWQESPVITSWDTWLPFCFDSQNLWWRRTLAITRQAAEELEGISFVSITDLGNSTDI
                     LAHLRGPQQLLLDTLENRYDVEWALAQITSLWFQWYEELLAITRTRQEGSCGWLGAWS
                     PKRTYPLQCDFSCMISPQTFRWMVMPEMERFVGRLDHPIYHLDGPNAICHLDALSQLP
                     HLKAIQWVPGDGNPSARYWPDLLKRILAMGKGVHVYVPAEDVEPLIDEVGPKGLFITT
                     GVGTPEEAVSLLRRVEIATARAAHRGVA"
     gene            complement(5969..6985)
                     /gene="aroF"
                     /locus_tag="P5179_05660"
     CDS             complement(5969..6985)
                     /gene="aroF"
                     /locus_tag="P5179_05660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRS94738.1"
                     /translation="MVVAMEQWATEEQIAAVIARLQEHKLDVHRSTGTSRTVIGAIGE
                     THMLDLQAFEVMPGVAEVVRISEPYKLASRTFKREDSVVYVGDVPIGGKEIVVIAGPC
                     SVESLDQTRRTAERVAAAGARMLRGGAFKPRTSPYSFSGLGEKGLEILRTVADEFGLK
                     VATEAMDRTQIDSVVAYADMLQVGARNMQNFTFLRELGKTRIPIMLKRGLSATYEEWL
                     MSAEYIMAGGNHNIVLCERGIRTFETYTRNTLDIAAIPVIKKLSHLPIIADPSHGTGR
                     RDKVIPMARAAVAAGADGLLVEAHVDPDHAFSDGVQTLNLDQFDELMGQLRLITPVLG
                     RTIR"
     gene            complement(7036..7332)
                     /locus_tag="P5179_05665"
     CDS             complement(7036..7332)
                     /locus_tag="P5179_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRS94739.1"
                     /translation="MDIADWRDRIDDIDRQLVVLLNERAKCSLNIGKLKRRMNLPIYD
                     PRRESVVLQQVVDLNEGPLTADAVKRLFERIIDENRRLERIYSALEESLQGNAT"
     gene            complement(7335..8168)
                     /gene="trpA"
                     /locus_tag="P5179_05670"
     CDS             complement(7335..8168)
                     /gene="trpA"
                     /locus_tag="P5179_05670"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRS94740.1"
                     /translation="MNEYNHNRCGPSRIHRAFASAKEQHRPAFIAYVTCGDPTLDRTR
                     QLAVEFAGAGVDILELGVPFTDPMADGTANQEAALRALAQGVTLRHVLRATEGLRGEG
                     FGLPIVLFTYYNPVFAYGVEKFAVDAVSAGVDGVLLVDVPVEEAQEFKPAFDGAGLGT
                     IFLVAPTTDDRRLPQILELTNGFVYCVSRSGVTGERNGLASGIRENVARVRRLTQLPV
                     AVGFGISSREQVSEIGQFADGVVVGSAIVRRIGEGGDTDEMVRQVTEFVRTLTGQFIG
                     G"
     gene            complement(8165..9436)
                     /locus_tag="P5179_05675"
     CDS             complement(8165..9436)
                     /locus_tag="P5179_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRS94741.1"
                     /translation="MKCGVATRCINPERPIRMAGYASRDKDSEGAYQDLNLKALALGD
                     GNATVVIVTSDILYFDDDVIGPVEVELARNPGIPPDRLFITASHTHCGPVTRRGDRDM
                     YGWRDEEYLDQIRQKLVESVREAVAEMAPCTLTYHRGRCGFNINRRVKTPSGVQMRPN
                     RDGAVDRDVDILCTRTSGQIAAILFGYCCHPTTMGGYLLGGDYPGFAKIGIESALPGA
                     TALFVQGCSGNVRPNNVDETGNFKNGPLEVVQKYGEELADAVLNVVAEPGEAIEGKLR
                     TGTRILELPLMPHPSRADLARATDDSSPFRRRWAWRMLDAYTRELPLPTTCSVHVQTL
                     QIGNALSLVAIAGEVCVEIGLRIKSLIGDGPRFVLGYTNRVIGYVPSKAIHADGGYEV
                     DGWYFYENMPSYLAADAEDIIVDAVGEMLAE"
     gene            9563..>9861
                     /locus_tag="P5179_05680"
     CDS             9563..>9861
                     /locus_tag="P5179_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94742.1"
                     /translation="MAGDRMDRFFGNDNDRPDEWDGQDGDFSDEDAEFPQTGLEIAIS
                     PFLGYEVELQGTFPIQLQIIRPATPNSARVLAGVYPDCIVVFDQEHYLVLPQTMV"
BASE COUNT         1934 a         3013 c         2781 g         2133 t
ORIGIN      
        1 cgagtggcgg ttcagggact gggtaattcc tacgttccgg cgcttggtca gcgagaggct
       61 catccccgac cgccacaacg tcgcgaaggt gacgccggtc gcgtaccacc tcccgcgttg
      121 cgaacggccg ctggaattcc acgcagtctc ggacgacctg gatttcgacc acggggcagg
      181 ccgcctcatc cgggggacgt tcggagtgta cgaccgtgcg cgggacccgg agctgattcc
      241 caacgatccg cgtttcgggt ggttccccgt tctgcccgtg aaaacggaca ggtcggtgct
      301 gacgcaattc aaacgtgttc tgcggccagg ggacgtcaat tcgccagagc atgcgcggga
      361 actgatgggg aagtactacc ccccggtcga ccgcggaacc gcatggagcc agatcgttgg
      421 tgacctcgtg ttcgcggcaa atacccacga aaactggttc gttccggaag aagtcaggct
      481 gcaggtccca cgccgtcctg cgaatgtgag aatcgaatcc actgggacgg cacaacttcg
      541 cctgcgctgg gagaaggccg aaggcgacaa cgcataccgc gtctggcgga tccgtgacgg
      601 cgtggaaaca tgcctgaccc cgaactcgat cgaagagacc gagtatctat tgccgcctgt
      661 ggaacaaggc gaccacttcg cggtctccgc gatcacttca gccacggaag aattcgcgca
      721 gaccctccac cttcatcagt ttctggtttt gaacagggcc gagagccgcc ggagtgaatg
      781 ggtaaatgcc gcgggcgaca ggatggaacg cttccgtttt gcggaatccg tcccgccagt
      841 atctgagcac gttctggaag cggaacggag atgcgccggt tgcttgcccg ttcaggacct
      901 cacctctcct ccggttaccc ccgacgaccc cttcgcgtct gtcaaactgg cgatcatgga
      961 ccacatggcc caatggaagt cattcgtcga agctgaagac ctcgacggaa tcatgagtat
     1021 gtacgcagcc gattactcag aaccggacgg ccgggggaag gactgggtgg aagccgcctt
     1081 ccggctggtt cttcgccgct acatggagag ccagtttgcc aggcttgtga aagagtgggg
     1141 tgccctgcca gggtggcgaa atcccgcgtt ccggcttctt gtcagggaat ggaggtcggt
     1201 gagcaatgat gcggcggagg tgctcgtcgt ggctcacatg tgggcaggcg gggggccaga
     1261 gatggaaccg agcgacatgt tcaaccaccc gttcggccgc ccgcacacga cgaccatgag
     1321 gtggaaactg atctccggag aatggaaaat aacgtcgacc gacccagcat ttcttcgtat
     1381 cgaggacaca gcgatctttc gcttccgtta ccaggggtgg taggagtatt gttgccagcc
     1441 ctccccggtt gacccgggga gggctcggtc tttgcgggcg gcttctacca tttcctcagg
     1501 aaatccagcg ccttcttcac gtctgcggca gggtcatccc cgacttcccg ctcgatggtg
     1561 tagaacccgc gatagccctg agctgtcatg agatcgatat actcttccca tggcactgca
     1621 ccctcgccca gaggaacttc ttttcccctc ccatcctcgc ggatgccatc ctttgcgtgc
     1681 gtgtgaacga tgtaaggtcc cagcgctctg acaccttcca gaacgtcgaa gcccatcatc
     1741 gcgaggttgg ccgggtcgaa gtttgccttc acagcctgcg ttttcaggct ttttatgaag
     1801 tccgcaagaa cgggacccgg ttccgggcca gtttccgttg cgaacattat gcccctgtta
     1861 tccgcatatg ccccgacatt gttcaatgcg tctgccatga tctgacggct tctggctttc
     1921 ggatcagcat ccacaacacc gatgtgagtg gtgatgatcg gcgttttcag gtccaccgcg
     1981 aggtctatca ctttctttgt gtctgttatg agttcgtcca ggccttcttc cttggtgtac
     2041 ggcttgccga aatcgccaca gagagcactg atgacaaggc cctggtccgc aacgaatttg
     2101 agaaactcct gtctgccagt ctttgagagg ttgtcagggt gcatctctcc gcccgtgaca
     2161 tagatctgga acccgtcggc accgatttct gccgctttct tgatgccgtt tcgcacgccg
     2221 agccggaatg actcgatcat gacacccact ttgaggttgc ccatcgcgtt tctcctgaat
     2281 ttgtggagtg agacactgtt tcgttgatgg tcgggagggc tattgaggca gcctcccgcc
     2341 catgttgtgc ggtgatgcgt acaagtgatc tgtccggatg cggaatgctt cagtcaatcc
     2401 ccatgcctca agggcttccg ccacgtccat gattcgatcg ccaaaccgct ccatctgacg
     2461 ctgcaattcc tgatggagac tggctcttgt ttccgttgcc aatggatcgc cggcgaggtt
     2521 ttcgagttgg taagggtccc tttcgtcatc gtagaggagc cattctccct gcgtatcaca
     2581 ggcatacgtg tagcgctcgg ttttgatccc gcgccacggc ttgattccgt aatgattcgc
     2641 ctgatccgtc gtgatgagat cggcgagata gagaacccgg ccttcttccg ttgcgccact
     2701 ccgtacacag gccgaaaggt cttcgccgtg catggagggt ggaacctgca ggtcaagaca
     2761 cccaagcagc gtcggaacta agtccagcag actgaaaagg gtcttgttca cgcgtccttc
     2821 tggaacagcc cccggccggt agaaaatcaa cggcactacg accgactccg cccagggacg
     2881 ttgtttgtgc gtgtgcccct gtgatcccag catggatccg tggtccgatg tgaacaccac
     2941 gagcgtgtcc ttttcgagat tgtttgcacg gaggtagtcg agcaggcgct gcgtttccgc
     3001 gtcaagggct gaaatgtgag cgtagtaccc tgcgagatgc cggcggttct ccggtgtgtc
     3061 cgggcaattt ggcggaagga ccagcgcgga aggatcgtac tgggtttccc ttcccggcgg
     3121 aagcggttcg tagggtgaat ggggtggccc ccatgaaagg aaaagacaaa acgggtgttc
     3181 gttgtcttgt tctctgtgcc gatccatgaa cgccatcgcg aggtctgtct gcacggatgg
     3241 ctcatacccc tccatcacaa ccggttccgg ggtatccagg taataacgcg ggttcatgta
     3301 atcatggtgg caattccaca cagcccagaa tgcgtcaaat cccagtctct ccgggcctgg
     3361 tggagtgaat ttgtaccgtg gaatgcctcc cagatgccat ttccctatgt agccgcaccg
     3421 gtaaccgtgc gagttcagga tgtgcgcgat gctcgtcaca tcggttcgaa ttgcgatgtc
     3481 gttcactacc gcaccgtggg ccgttggcca gaggccggtc aacattgtcc ctctggcggg
     3541 cgtgcacaca ggcgtattgg aatacgcgcg cgggaaacgg catcctcgcg aagccatgga
     3601 gtcgagacac ggtgtggata cgttcgggtc acctgcgcaa cccatcgcct gtccccgcat
     3661 ttcatcgccg aataccacga ttacattggt ggccaccgtc gctcgcctcc atcatctcaa
     3721 acgcgcggga aatccttctt tatccagcgt gcgtatcgcc gcatctgccg tctggatgtc
     3781 tttgaacgcc atgcggaatg taccgccctc gccttcgcgg attttcagca gctcgatgtc
     3841 catgatgttg atctgttcgc gtgcaagagc cgccgtcatg cgggcaagct gccccggccg
     3901 atcctcgcag cgcacaagca catcgcacag aggagaaaga aaccctttcg tatctctggg
     3961 gattgagctc cgtatctcat tggctgcgcg aaaatgcggt tccatatgct cgatatctgc
     4021 cagcacctgc tcgagttggc cggcgaagcc gcggagcgcc tttcgtattt ctgaggcatt
     4081 tgtcgccaga atgtcctgcc agacgctgta cggacttgac gcaattctgg tcatgtcacg
     4141 gaatccgccc gccgccattt gaaggtgcgc cggctcgatt gcgttcagtg tgccgacgta
     4201 ttccacaagg gcgagcgcta tcatctgcgg aaggtggctc acagcggctg ccactcggtc
     4261 gtgagcggaa gcggacaggc agaatactcg tgcaccgatg ctcgcgatgc gctccgtgaa
     4321 agacgcgcgc acactctccg gcacggaccc ggagggggtg agcacataca gcgcgttctg
     4381 gaacagaaac ggatccgctg caccgactcc gtttcgttcc gagcccgcca tggggtggcc
     4441 cccgataaaa accgagtgct gcggcatgca tttctcggcc cgttccacga tctctttttt
     4501 cgtgcttccg acgtcagaaa caacggtgcc gggagtgatg acgtgggcca cctccggcag
     4561 cagttcgatg atccgagaaa cgggagtgca aagaaacacg gccttcgttt cgcgcaatgc
     4621 aagggcgagg tcgttatatt cccatccctc gtctattgcg cccagaccga gtgcctcctt
     4681 gatcgttttt cggctgctga ttccgaccac acggccggtc gcgccaaatt ccttcagcgc
     4741 aagggcgagc gatcctccaa tcaggccgac cccgacgatt gcaactgatg agaaatccgc
     4801 ccctggacgt tcatccatcg tcaggcgact ccccgatgtg cggcacgcgc ggtcgcgatt
     4861 tcaacgcggc gcaggagtga tactgcctcc tcgggtgtcc ccacgcccgt cgtgatgaaa
     4921 agccccttcg ggcctacttc gtcgataagg ggctcgacgt cttctgccgg gacgtacaca
     4981 tgaacacctt tgcccatcgc gaggatccgt ttgagaaggt ccggccagta acgcgcggag
     5041 ggattgccgt ccccgggaac ccattggatc gccttgaggt gcggcaactg cgacagtgca
     5101 tcaagatggc agatcgcgtt cggaccgtcc agatgataga tgggatggtc caatctgccc
     5161 acgaaccgtt ccatctccgg catgaccatc catcggaagg tctgcgggga gatcatgcac
     5221 gagaaatcgc actgcagtgg gtaagtccgt ttcggtgacc atgcgcccag ccatccgcat
     5281 gagccttcct gtcgcgttcg ggtaattgca agcaattcct cgtaccactg aaaccagagg
     5341 gaggtgatct gcgccagcgc ccactcaacg tcataccggt tctccagtgt atctagaagg
     5401 agttgctgtg gcccccgcag atgcgcgaga atatcggtcg aattcccgag gtccgtgatg
     5461 cttacgaatg agattccttc cagctcttcc gccgcttgcc gcgtgatcgc aagggtccgc
     5521 cgccaccaca ggttctgaga atcgaagcag aacggaagcc acgtatccca ggatgtgatt
     5581 accgggcttt cctgccatgc ggtagtctcc gcaaaggcaa gctcgcaccc cagataggcg
     5641 cttcctacgg agggccccag attgacgaac atcagaggga aggattcgcc accgtagtag
     5701 gtggtctcgg cgtgcgcctt ctggcgggca atcaggtaat cggcatttgt ccagcgttcc
     5761 accatgctcg cgggagtggg gatctccttg cgtggccggc cgttatgtgc cgtaacagct
     5821 accaccgcac gatcgatgat ttcgttgttc caccacgcct cgattcgggt gcgtgccttt
     5881 tcccagtcgt ctttgcagcg gaattccacc tgtgtccctc cttgaatttc acgccgcccg
     5941 aaagatgatg cggtagcccg cgtcgcgctc agcgaattgt tcgaccgagg accggggtaa
     6001 tgagcctcaa ctgccccatc aattcgtcga actgatccag attcaatgtc tgcactccat
     6061 ccgaaaacgc gtggtccggg tcgacgtggg cctccacgag caatccgtca gcccctgcag
     6121 caacggcagc ccgtgccatc gggattacct tgtcccttcg gcccgttcca tggcttggat
     6181 cggcgatgat tggcaggtga ctcagcttct tgatgacggg tattgccgca atgtcgagcg
     6241 tattgcgcgt atacgtctcg aacgtccgta tcccgcgttc gcagagcacg atattgtggt
     6301 tgcccccggc catgatgtat tcggcagaca tgagccactc ttcatacgtc gcagaaagtc
     6361 cccgtttcag catgatggga atgcgcgttt tcccgagttc gcgcagaaag gtgaaattct
     6421 gcatgttgcg ggcgccgacc tgaagcatat ctgcgtatgc gacaaccgaa tcgatttgcg
     6481 tgcgatccat ggcttccgtg gctactttca gcccaaattc gtcggcaacc gtgcggagga
     6541 tctccaggcc tttttctccc aatccagaga aactgtacgg ggatgtgcgc ggtttgaatg
     6601 caccaccccg aagcatgcgg gccccggccg ctgccacacg ttccgccgta cgtctggtct
     6661 ggtccagaga ctcaacggaa cacgggccgg cgatcacgac tatctctttg ccgcctatcg
     6721 gcacgtcccc gacgtagact acgctgtcct cccgcttgaa cgtgcggctg gcaagtttgt
     6781 agggctcact gattcgtacc acttcggcga caccaggcat gacttcgaat gcctgcagat
     6841 cgagcatatg tgtttcgccg atcgcaccga tgacggttcg cgacgtgccg gtggatcgat
     6901 gcacgtccag tttgtgttcc tgcaaacgcg caataacggc cgcaatttgc tcttcagtcg
     6961 cccattgttc catcgccaca accatggcgc attacctcct gtatggtcag atgtgttcag
     7021 aatccgctcg gtgaatcaag ttgcattccc ttgcaggctt tcttcgagtg cgctgtaaat
     7081 gcgctccaac cgccggttct catcgatgat tcgttcaaac aagcgtttga cggcatccgc
     7141 cgtcaacggc ccctcgttca gatccacaac ctgttgcagg acaacacttt cacggcgtgg
     7201 atcgtaaatc ggcaggttca tccgacgctt cagcttgccg atgttcagcg agcacttcgc
     7261 gcgctcgttg agcaacacga caagctggcg gtcgatatcg tcgattctgt cgcgccagtc
     7321 ggcaatatcc atgttcatcc cccgatgaat tggccggtga gtgtccttac aaattcggtt
     7381 acctggcgga ccatttcgtc ggtgtctcca ccttcaccga tgcgccgaac tatcgcactg
     7441 cccaccacca cgccgtcggc aaactggccg atctcggaaa cctgttcgcg ggaggagata
     7501 ccaaatccta cggccacggg aagttgcgtc aaccggcgga cgcgagccac gttctctcgg
     7561 atgccggagg ccagaccatt ccgttcaccg gtgacgccgc ttcgcgaaac gcagtagaca
     7621 aacccgtttg tcagctcgag gatttgcggc aaacggcggt cgtccgtggt cggagcgacg
     7681 aggaaaatcg ttcccagtcc cgccccgtca aacgccggtt tgaactcctg cgcctcttcc
     7741 accggaacat cgacgagcag cacgccatct actcccgccg aaaccgcatc tacggcgaac
     7801 ttctccacac cgtacgcgaa tacggggttg tagtacgtga agaggacgat tggcaacccg
     7861 aagccctcgc cacgaagccc ttccgtcgcg cgcaaaacgt ggcgaagcgt tacaccctgc
     7921 gcaagcgcgc gcaaagcggc ttcctggttc gcggtgccat ctgccatcgg gtccgtgaaa
     7981 ggcacgccca gttccaggat atccacgccc gccccggcaa attcgaccgc aagttgtctc
     8041 gtcctgtcca gcgtcggatc accgcaagta acatacgcga tgaacgcggg acggtgttgc
     8101 tctttggccg acgcaaaggc gcgatgaata cgggaaggtc cgcagcggtt gtgattgtat
     8161 tcgttcattc cgccagcatc tcccccaccg cgtccacgat gatgtcctcc gcgtcggcag
     8221 cgagatacga gggcatgttt tcgtagaagt accagccgtc aacttcgtac ccaccgtcgg
     8281 catggatggc tttcgaaggg acatacccga tcacccggtt tgtgtaaccg agaacgaacc
     8341 gcggtccgtc cccgatcagg gacttgatcc gcaagccgat ttccacacac acttcgccgg
     8401 cgatggcgac cagtgaaagg gcgtttccga tttgcagagt ctgcacatga acggagcagg
     8461 ttgtgggaag gggcaactcc cgggtgtacg cgtccagcat gcgccacgcc caccgcctgc
     8521 ggaacggcga gctgtcgtcg gtcgcgcggg cgagatcagc acgagacgga tgcggcatca
     8581 agggaagctc gagaatccgt gtccctgtcc tcagctttcc ttcaattgct tcgcccggtt
     8641 ctgccaccac gttcaggact gcgtccgcca gctcctcacc gtatttctgt acaacctcaa
     8701 gaggcccatt tttgaagttt cccgtctcat ccacgttatt ggggcgcacg tttccactgc
     8761 acccttgtac gaaaagggca gtcgctcccg gcaaagcgct ctcgattccg atcttcgcga
     8821 agcccgggta gtctccgcca agaaggtacc cgcccatcgt ggtgggatgg caacagtaac
     8881 cgaacaaaat cgccgcgatc tgcccgctcg ttcttgtaca caggatgtca acgtctcggt
     8941 ccaccgctcc gtcgcgattc gggcgcatct gcacacccga aggcgttttc acccgccggt
     9001 tgatgttgaa cccgcaccgg ccgcggtggt acgtcaacgt gcacggtgcc atctccgcca
     9061 ctgcttcgcg aacactctcc accagcttct ggcggatctg atcaagatac tcctcatccc
     9121 gccacccgta catgtcccga tcaccgcgcc gggtgacggg cccgcaatgc gtgtgggaag
     9181 cagtgatgaa tagcctgtcg ggggggattc ccgggtttcg cgccagttcc acctccacgg
     9241 gtccgattac atcatcatcg aaatacagga tatccgaggt gacgataacc accgtggcgt
     9301 tcccgtcacc caaagccagt gctttcaggt taagatcctg atacgcacct tcgctgtcct
     9361 tgtctcggga cgcatacccc gccatccgga tcgggcgctc gggattgatg caacgagttg
     9421 caaccccgca cttcatggtg ttcccatcct ttcgattgcc tcgttgcgat atctgcctgc
     9481 tttgttggcc ggacatcacg gactgccgta cattttgtga agaccaaccc acgaattgcc
     9541 cccgtcccat gagaggacat atatggccgg cgaccgtatg gaccgtttct tcggtaacga
     9601 caacgaccgc cccgatgaat gggacgggca agacggtgat ttctctgacg aggatgcgga
     9661 attcccgcag accggtctgg aaatcgcgat ttcacccttt ctggggtacg aagtcgaact
     9721 gcagggcact ttcccgatcc agctccagat aatccgcccg gccaccccca attccgcgcg
     9781 tgtacttgcc ggtgtatatc cagattgcat cgtggtgttc gatcaggaac actacctcgt
     9841 cttgccgcag acaatggtca t
//
LOCUS       DAOTNG010000086         9842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6378_length_9842_cov_4.666701, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000086 DAOTNG010000000
VERSION     DAOTNG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_9842_cov_4.666701"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="P5179_05685"
     CDS             <1..997
                     /locus_tag="P5179_05685"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRS94743.1"
                     /translation="EVQEQIHALFDGHVRELCQMNFALGELFAEAALNVIRSAGFAPA
                     DVHLIGSHGQTVYHVPRGEGRTASSLQIGEPAVIAERTGITTIGDFRPRDIATGGEGA
                     PLVPYADWVLCRRNEEAIALQNIGGIANLTVVTPRLEDVRAFDTGPGNMVIDGAVTLM
                     TRGSRTYDEDGRIAASARPDEGLVRDLLDDDYFRQLPPKSTGREQWGRQFVRGVIERR
                     GVRADAALVSTLTEFVARSIADAYARFVLPHVSLSFVYLSGGGARNPVLRQRMTELLA
                     PVPVGDSSVLGLPVDGKEAIAFAVLANETVCGEPSNVPGATGARGRRVLGKIVPA"
     gene            complement(1028..1594)
                     /locus_tag="P5179_05690"
     CDS             complement(1028..1594)
                     /locus_tag="P5179_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94744.1"
                     /translation="MTFSRNPAFIAAIILLALQGCERTRVTPPVEMRNLRVTPTKIGL
                     DISFELTDSKGQPTIMMFGNGVVRIDDSPDSVQKEVFPGHPAGSSVLDSTWLLYNARL
                     FVTREHFVILRGGDSTRGQDKYLCALGVYPYSHFLRKPRRPTGNVRVALVGMDGRRVL
                     LGHAPVIWPSEAFSATSGTGDQSRFGNR"
     gene            1939..4092
                     /locus_tag="P5179_05695"
     CDS             1939..4092
                     /locus_tag="P5179_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94745.1"
                     /translation="MTFLTGHFSCVVFRSISGFVLLVLLLASSRSVAEELIPAAGAPA
                     NALRIPPGRLAVPPRIDGIWTPAEWSGAIRVDDFHCHDWNRAPTESTVVWMASDDRGL
                     YFFARCFDRNPDKLRMEERKRLQLAYKGNAVPSLSDDFISFEIDAENLRRSNGAYAFT
                     VTPRGTQADFIPDGAATKIEWRGDWTAAARIDSAGWTVEVSVPYSMLGLPSGKNTVGV
                     SIRRWVPRLQEESRWPNMGFAWDRTRIAHWENLDVGSTGRAFPLVMPYGVAQARDGRF
                     DGYVGLDVKKTFPSGQTFIGCVYPDFRNVENDVLGLDFSYSEKQRADSRPFFVEGYRF
                     LPDAWMMYGQRIGEVYGGGKYFGQIRNHRIGVLTVYDREEVLHSAARWYWQPVPRLEF
                     ENNVVWRHAPPNTPVRSGVPMVEDNLLFVSLVKKSRMIGSLTEYMRLQTGFAQSDGDS
                     ADGFNLEGDYQIIPTASGPSLVVKARHLGNGMTPVDGLLDPMDFDQRDASIELGYERT
                     ADRQWFREWNVSGFVHRSVRSNGNLYLQRYTVESWTKARGGTELSTEFRVEERPPYRD
                     WTFLHEFGWKQDQTYQKGGISARWGWLQGADYLLVTLDQGIHSWDRVTASARLQFRRR
                     DFPAGHALRPTGGVEDRYQVVTTVQYDLTAERSISGRLTYNDDGSGNAWKSRFNGYCS
                     YRQLVRNGFDLFLIVGDPSAETWTRRVAIKAMVVL"
     gene            complement(4117..5889)
                     /locus_tag="P5179_05700"
     CDS             complement(4117..5889)
                     /locus_tag="P5179_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS94746.1"
                     /translation="MRRVTFAWHLVPVVVLIVWGASLLSAMYAARAIRTFHAGQTGAE
                     LAAQAKLVRQVITPFLVQANPASVDSICKVFGVASSSRITVVSKSGIVLGETGPIAAR
                     LDNHADRPEIIAAFSGRTGSAVRFSHTTRRTMMYTAIPVFQGGEPVAAVRVAKPIDSI
                     EFLVVHLQWRFFLAGAVVALVATVAVLIAIRRATDPLETMRRVAHAYSQGDFRESLPT
                     SRVSEVDDLAQSLNAMARQLDERIRSLVRQRNEQEAVLSSMVEGVIAVDSEERVINLN
                     LAAARLVGADIARSHGRLIQEVVRNTELQQIIRRALDSHEPVEGEIAIRSEPRVFLQA
                     HGTVLRDGTGECIGALVVLNDITRLRTLENMRKEFVANVSHELKTPITSIKASVETLL
                     DGAMENREMARRFLDIVVRHADRLNAIVDDLLSLSRIEQEAENRQIELDALDVEPVLQ
                     AAVQSCLVTADERGINIQVSCANDLKARMNAPLIEQAVVNLIDNATKYSDPGGVVHVV
                     AEQKGTEVRIDVRDTGCGIAPEHLQRVFERFYRVDKARSRNAGGTGLGLSIVKHIVQA
                     HGGRVTVESALGKGSTFSLFLPAA"
     gene            complement(5894..6580)
                     /locus_tag="P5179_05705"
     CDS             complement(5894..6580)
                     /locus_tag="P5179_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS94747.1"
                     /translation="MPGQHVAIIEDEDDILELISYNLRKEGYRVTTATSGEAGLLAVR
                     NTLPDLVLLDLMLPGVDGFEVCRSLKKSAETSHIPIIMLTAKGEETDVVAGLELGADD
                     YITKPFSPRVLLARVRAILRRKPKTEDEGSSPIVVHDVAIHPGRHEVTVGGVHVALTH
                     TEFNLLHLLASRPGWVFTRYQIVEAIRGDNSPVTDRSVDVHVVGLRKKLGQAARYIET
                     VRGMGYRFKG"
     gene            6770..7600
                     /gene="proC"
                     /locus_tag="P5179_05710"
     CDS             6770..7600
                     /gene="proC"
                     /locus_tag="P5179_05710"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRS94748.1"
                     /translation="MDTTLRIGIIGGGQMGTALVKGILHAGVADASEITVSDVIASAR
                     ERLHAESGVRTTNDNTEAAKNADVVVIALKPQVAAEALEPVAGKLMPTQTVLSIMAGV
                     PTGKLERWLSGDDEAANPVHVVRAMPNTPALIGEGMAAISRGAHATDADMNRAERILA
                     AVGRVVRVDEKQLDAVTGLSGSGPGYVYTVIEALADGGVKVGLPKPLALELAAQTVLG
                     AARMVLETEEHPAVLRDRVTSPGGTTIAGLHAMEAAGIRNALMQAVEAAARRSRELGE
                     "
     gene            7667..8632
                     /locus_tag="P5179_05715"
     CDS             7667..8632
                     /locus_tag="P5179_05715"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRS94749.1"
                     /translation="MQDTSGNRRKVVVVGAGAVGATFAYALAQSGLADEIVLIDRNED
                     LVRGQVLDLAHGQPFFPTVSIRKGDASDYASASLIVITAGAAQRPGETRLQLLQKNAA
                     VIKGVMTDIIEQASPAVILVVTNPVDVMTYVAIETSGWDRSRVIGSGTVLDSARLEHL
                     LSAHCGVDVHNVHGFVLGEHGDTEFVAWSLTHIAGMQMDAYCPICKGCADNLAEREAI
                     ARQVRDSAYHIIGYKGATWFAVGLALVRIAGAILRNQRSVLTVSALLHGEFGLSDVCI
                     GVPCIVSARGIERVVESVLSESELAALHSSARTLKKAVEQLHAGA"
     gene            9032..>9842
                     /locus_tag="P5179_05720"
     CDS             9032..>9842
                     /locus_tag="P5179_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRS94750.1"
                     /translation="MENQEHVVIAGAGPAGLASAITLARAGLSVEVRELRGDVGGHFR
                     GNLQLLTNYPDLEDARTELCSLLGSVPVSLWPQRSAQLFGPSGKCVSGRSSEPFGYLL
                     HRGPDPTALDGALKRAAEEAGVQFRFHTRARPEEVDIVATGGAAAQGVAREWFGRTEL
                     PDGFHAVFDNRICPGGFGYLLVAEGYTVVGAAVVRHRERAQEAFHKTVRFFRERAGVP
                     RIEHPTLSGSLDLFLPDPERTNMRPLHVGEAGGFADHLFGFGIRLALQSGVL"
BASE COUNT         1912 a         2773 c         3125 g         2032 t
ORIGIN      
        1 cgaggtgcag gagcagattc atgctctttt tgacggccac gtgcgtgaac tgtgccagat
       61 gaactttgcg ctcggggagc ttttcgccga ggcagcactc aacgtgatcc gatctgccgg
      121 atttgcgccg gcggatgtcc atctgatagg ctctcacggg cagacggtgt accacgtgcc
      181 acgaggagag gggcgcacgg cctcctctct tcaaattggt gagccggccg taatcgcaga
      241 gcgcacgggt atcaccacga taggagactt ccgaccgcgg gatatcgcca ccggaggcga
      301 gggagcgccg cttgttccat atgcggattg ggtgctgtgc cggcggaacg aggaggcgat
      361 tgcccttcag aacatcgggg gaatcgcaaa tctcactgtg gtgacgccgc ggctggagga
      421 tgtccgcgcg tttgacacgg ggccggggaa catggtgatt gacggggcgg tgactttgat
      481 gacccgtgga tcgaggacgt atgacgagga tggaagaatc gccgcgagtg cgcgcccgga
      541 tgaaggtctg gttcgggatc tgttggacga cgactatttc cggcaactgc ctccgaaaag
      601 caccggaagg gagcagtggg gcaggcagtt cgttcgtggc gtgattgagc gcaggggtgt
      661 gcgtgcggac gcagcgctgg tgagcaccct tactgaattc gtggcccggt ccattgcgga
      721 tgcgtacgcg aggttcgtac ttccccacgt ttcgctgtca ttcgtctacc tttcgggagg
      781 aggggcaagg aacccggtgt tgaggcagcg gatgaccgag ttgctcgccc cggtaccggt
      841 gggggactcg tcggttctgg gtttgccggt ggacgggaag gaagccatcg cgttcgcggt
      901 tctcgccaac gagacggtct gcggcgaacc ttccaatgta ccgggcgcga ctggagcgag
      961 gggccgtcgg gtactcggca agatcgtgcc ggcgtgaggt gcaggaggcc cggaagcgca
     1021 ggtctggtca acgattgccg aagcggctct ggtcgccggt tcctgatgtc gcgctgaatg
     1081 cttcagatgg ccagataacg ggtgcgtgcc ccagaagaac gcgtctgcca tccattccca
     1141 ccagcgctac ccgcacgttg cccgtggggc gcctcggctt cctcaggaaa tgggaatagg
     1201 ggtacactcc cagcgcgcag aggtatttgt cttgcccgcg cgtcgaatcc ccgcctcgca
     1261 ggatcacaaa atgctcgcgc gttacaaaaa ggcgtgcgtt gtacaacagc cacgtcgaat
     1321 cgaggactga gctccccgcc ggatgcccgg ggaagacctc tttctggacg ctgtccggag
     1381 aatcgtcaat ccgcacgaca ccgttcccga acatcatgat cgttggctgc cctttggagt
     1441 cggtcaactc gaagctgatg tcgaggccga ttttcgtggg agtcacgcgc aggttccgca
     1501 tctcaacggg tggtgtgacc cgtgtccttt cacatccctg cagggcgagc aggataattg
     1561 ccgcaatgaa ggcgggattc cggctgaacg tcatcgggag ttccctgaag aagtcaaggg
     1621 atatccaaag ggatcggtct gaatttacga tgcggtgagg tgtggaagca cgcggggacg
     1681 acgcggcgtg gtgtgcctcg ttttgcgata gagagcgtga accacagcga ggatcgcgaa
     1741 taccgacacg tagaaacgaa tggacaaccg gttttcaaaa tcacctcaac gggccctcgc
     1801 ggtgcgagac gggttggggc gtcccgacga cgggtccgaa tttttacaaa cggcatggca
     1861 gcctgacaaa tccgggcagg gcttcggcgt cgcgaaggac gatgccgggg tttcgagacg
     1921 cccgcaatcg aggtgagggt gacatttttg acagggcatt tctcctgcgt agtgttccgt
     1981 tccatttccg gcttcgtcct ccttgtactc cttctggcgt catcgcgttc ggttgccgag
     2041 gagctgattc ccgcagcggg tgcccccgcc aatgcgttgc gcattccacc gggccgcctg
     2101 gcggtacctc cccgaattga cggcatctgg accccggcgg aatggtcggg cgcgattcgt
     2161 gtcgatgact tccactgcca cgactggaac cgggctccga ccgaatccac tgtagtgtgg
     2221 atggcctctg atgaccgcgg cctctatttc ttcgcgcggt gctttgacag gaaccccgac
     2281 aagcttcgca tggaagaacg aaagcggctg caactggctt acaagggcaa cgccgtgcct
     2341 tctctgagcg acgatttcat ctcgtttgag atcgacgcgg aaaacctccg gcgaagtaat
     2401 ggcgcgtacg cgttcacagt tacccctcgc ggaacgcagg ccgatttcat tcccgatggc
     2461 gctgcgacga agatcgaatg gcgcggggac tggaccgcgg ccgctcgcat cgactccgct
     2521 ggctggaccg tggaggtttc cgtgccgtac agcatgctcg gactcccctc ggggaaaaac
     2581 acggtgggcg tttcgatccg gaggtgggtg ccccgcctgc aggaggagag ccgctggccg
     2641 aacatgggat tcgcatggga tcgaacgcga atcgcgcact gggagaacct cgacgtgggc
     2701 tcgacgggcc gggctttccc actggtgatg ccgtacggcg ttgctcaggc gcgtgacggg
     2761 cgttttgatg ggtacgtagg gttggacgtt aagaagacct tcccctccgg tcagacgttt
     2821 atcgggtgtg tgtatcccga ttttcgaaat gtggaaaacg acgttctcgg gctggacttc
     2881 tcgtacagcg aaaagcaacg cgctgacagc cgaccgtttt ttgttgaggg ataccggttc
     2941 ctgccagatg cgtggatgat gtacggtcaa cgcatcggag aggtgtacgg cggtggcaag
     3001 tatttcgggc aaatccgcaa ccaccgcatc ggcgtactga cagtgtacga ccgcgaggag
     3061 gtcctccatt ccgccgcccg gtggtactgg cagcctgttc cgaggttgga attcgagaac
     3121 aacgtggtat ggcgccatgc acctccgaat acgccggtgc gctccggcgt gccaatggtc
     3181 gaagacaatt tgctttttgt ctcgctggtg aagaaatctc gcatgatcgg gagcttgacc
     3241 gaatacatgc gcctgcagac aggttttgcc cagagtgatg gggacagcgc ggacggattc
     3301 aacctggaag gggattacca gattattccg accgcaagtg ggccgtcgct ggtggtgaag
     3361 gcccgccacc tgggcaacgg aatgacgccg gttgacgggc tgctcgaccc gatggatttc
     3421 gaccagcgcg acgcttccat cgagctgggc tacgagcgca cggccgaccg gcagtggttc
     3481 cgcgagtgga acgtatccgg gttcgttcac cggtccgtcc gcagcaacgg caacctctat
     3541 ctccagcgct ataccgtcga atcgtggacg aaagcacggg gaggaacgga gttatccacg
     3601 gagttccggg ttgaagaacg cccgccgtac cgcgattgga cgtttctcca tgagtttggg
     3661 tggaagcagg accagaccta ccagaaggga gggatttcgg cccggtgggg atggcttcag
     3721 ggggctgatt acctgcttgt gaccctcgat cagggtatcc actcgtggga tcgcgtcacc
     3781 gcgagcgcgc gcctccagtt tcgcagaaga gatttccctg cgggacacgc attacgaccg
     3841 accggaggtg tggaagaccg ttaccaggtg gtgacgaccg tgcagtatga cctgactgct
     3901 gaacgaagca tttcgggcag actcacgtac aacgacgacg gctccggcaa cgcatggaag
     3961 agccgtttca acggctactg cagctaccgc cagcttgtgc ggaatggatt cgatctgttt
     4021 ctgatcgtgg gtgatccgag cgcagaaacg tggactcgcc gggtggcaat caaggccatg
     4081 gtggtgcttt gaggggtgtt gccagcctct ttcagtctat gcagcgggaa gaaacaggga
     4141 gaacgtactg ccttttccca atgcgctttc caccgttact cgacctccgt gagcctgtac
     4201 gatatgtttg acgatcgaaa gtcccaatcc tgttcctccg gcgttccgac ttctcgcttt
     4261 gtccacccgg taaaatcgct cgaagactct ctgcaggtgt tcaggcgcaa ttccgcaccc
     4321 ggtatcccgg acatcgatcc tgacttctgt gcccttctgt tccgccacga cgtgcacgac
     4381 cccgccaggg tcgctgtatt tcgtcgcgtt atcgatcagg ttcaccacgg cctgttcgat
     4441 caggggagcg ttcattcttg ctttgagatc atttgcacat gagacctgga tgtttattcc
     4501 acgttcatct gcagttacga ggcatgactg gaccgcggcc tgcaataccg gctccacatc
     4561 gagggcatcc aactctatct gccggttctc cgcttcctgt tcgattcgag agagactcaa
     4621 caggtcgtct actatcgcat tcagcctgtc ggcatgccgc accacgatgt cgaggaacct
     4681 tctcgccatc tcccggtttt ccatcgcgcc atcgagaagc gtttcaaccg aggccttgat
     4741 ggaggtgata ggggtcttca gttcgtgtga aacgttggct acgaactctt ttcgcatatt
     4801 ctcgagtgtg cgcaggcggg tgatgtcgtt cagaacgacg agagcaccga tacattcacc
     4861 tgtcccgtcg cgaagtacgg ttccgtgcgc ctggaggaag acccgcggtt ccgaccggat
     4921 ggcgatttcc ccctcaaccg gttcgtggct gtcgagggct cttcgaatta tctgctgaag
     4981 ctcagtgttc cgcaccactt cctggatcaa tcgtccatgc gatcgcgcaa tgtctgctcc
     5041 cacgagcctt gccgccgcaa ggttcaggtt gatcacacgc tcttccgagt ctaccgcgat
     5101 gacgccttcg accatgctgg aaagaacggc ctcctgctca ttccgctgac gaaccagcga
     5161 gcgtatccgc tcatcgagtt gtctggccat cgcgttgagc gattgggcaa ggtcgtcaac
     5221 ctcagacacg cgtgatgttg gaaggctttc gcgaaaatca ccctgcgaat acgcgtgggc
     5281 gacccgccgc atcgtctcca gcggatcggt tgcccttcgg atggctatca gaacggcgac
     5341 agtggctacg agggccacca cggcacctgc gagaaagaaa cgccactgga ggtgaacaac
     5401 cagaaattcg atgctgtcaa tgggcttcgc cacgcgaacc gcagccaccg gttcaccacc
     5461 ctgaaacacc ggaatcgccg tgtacatcat cgtccggcgt gtagtgtggc tgaatcgtac
     5521 cgcagagcca gtccttccgg aaaatgctgc gatgatttcc ggccggtcgg cgtggttatc
     5581 aagccgtgcc gcgatcggac cggtttctcc cagaactatg ccggacttgc ttaccaccgt
     5641 gattcgagaa gaagaggcaa ccccaaaaac cttgcagatg ctatcgactg aagcaggatt
     5701 cgcctggacg agaaacgggg taatcacctg tctgaccaat tttgcctgcg cggcgagctc
     5761 ggcacccgtt tgcccggcgt ggaacgtgcg gatggcgcgg gcggcataca tcgcggagag
     5821 gagcgacgcg ccccacacga tgagaacgac cacgggcaca aggtgccatg caaacgtcac
     5881 gcggcgcatg cgttcaacct ttgaagcggt aacccattcc ccgcaccgtt tcgatataac
     5941 gtgcggcttg cccgagtttc ttcctcaagc cgacgacgtg aacgtccacg gagcggtctg
     6001 taaccggaga attgtctccc cgaatggcct cgacgatctg atagcgcgtg aaaacccagc
     6061 cgggccgact tgcgagcagg tggagcaggt tgaattcggt gtgtgtaagc gcaacatgaa
     6121 caccgccaac ggttacctca tggcggcccg gatgaatggc cacatcgtga acgacgatcg
     6181 gggacgagcc ctcgtcctcc gttttgggtt tgcggcgcag aatggcccgg acgcgcgcga
     6241 gcaagacacg tgggctgaac ggtttggtaa tgtaatcgtc cgctcccagc tcaaggccgg
     6301 ccactacgtc agtctcttcc ccttttgccg tcagcatgat gatcggtatg tgcgaggttt
     6361 cggccgattt tttcaacgac ctgcacactt cgaatccgtc gacgccaggc aacatcagat
     6421 cgagcagcac aaggtctgga agagtgttcc tgaccgccag caaacccgct tccccgcttg
     6481 tggcggtggt tacacggtag ccttctttgc gcaggttgta cgaaatgagt tccaggatgt
     6541 cgtcttcgtc ttcgataatg gcaacgtgtt gtcccggcat cgcataaccc tcagctcttg
     6601 ttgtacgatg tataccacga gttcataatc tgtccaagga gtgttaagat tgaatgaaaa
     6661 tgccacagaa gggccggcgt gtttcgcgtt ccccctgtct gttcggcgcc caaccgtggt
     6721 accattggaa tgaaaacaac cagttcgcgg aaaagacagg agcgctgaca tggacacgac
     6781 tctccggatt ggaattatcg gtggcggcca gatgggtacg gcgctggtca aaggaatact
     6841 ccacgccggt gtcgcggatg cgagcgagat cacggtttct gacgtcatcg cgtcggcgcg
     6901 ggaacgtttg cacgcagaga gtggcgtcag gacgacgaac gacaacaccg aggccgcaaa
     6961 aaacgcggat gtggtcgtga tcgccctcaa gcctcaggtg gctgcggagg cgctcgagcc
     7021 ggtggccggc aaactcatgc cgacgcagac ggttctctcc ataatggccg gcgtaccgac
     7081 cggcaagctg gagcgatggc tctctggcga cgatgaggcg gcaaaccccg ttcatgtcgt
     7141 tcgagcgatg ccgaacaccc ctgcgctgat tggcgagggg atggcggcga taagccgtgg
     7201 tgcgcacgca acagacgccg acatgaaccg cgcggaacgg atactggcgg cagtcgggcg
     7261 ggtagtccga gttgacgaga aacaattgga tgcggtaaca gggctgtctg gcagcggccc
     7321 cggatatgtg tatacggtta tcgaggcgct ggccgacggt ggcgtgaagg tcgggctgcc
     7381 aaagccgctc gcgcttgagc tggctgcgca gaccgtgctc ggcgccgcac ggatggtgtt
     7441 ggagacggaa gagcatccgg ccgtgctgcg ggacagagta acatcgccgg gtggaacgac
     7501 gatcgccggg ctccacgcca tggaagccgc gggcattcga aacgccctca tgcaggctgt
     7561 cgaggctgcg gcgcgacgtt cccgtgagct gggcgagtaa agcgaatttg ccgctcgccg
     7621 attttcctgg actcgtgaag gtaacgccag attgaggaag acacggatgc aggatacctc
     7681 aggaaaccgt cgaaaggtcg ttgtggtggg tgcgggagcg gtcggggcga cgtttgcgta
     7741 cgctctggcc cagagcgggc tcgccgatga aatcgttttg attgacagaa acgaggatct
     7801 tgtgcgcggt caggtgctgg atctggccca cggccaaccc tttttcccca cggtttccat
     7861 tcgcaagggc gacgcttctg actacgcctc cgcgagtctg atcgtgatca cggcgggggc
     7921 ggcccagcgc ccgggagaaa cacgcctgca actgctccag aagaatgctg ccgtcatcaa
     7981 aggcgtgatg accgacatca ttgagcaggc ttccccggca gtgatcctcg ttgtgacgaa
     8041 tcctgttgac gtgatgacgt atgtcgcaat cgaaacctcg gggtgggacc gctcaagggt
     8101 tatcggctcc gggacggttc tcgacagtgc gcggcttgag catctgctga gtgcccattg
     8161 tggagtggac gtccacaacg ttcacgggtt tgttcttggc gaacacggcg acaccgaatt
     8221 tgttgcatgg tctctcactc acatcgccgg catgcagatg gacgcatact gccccatctg
     8281 taagggatgc gccgacaacc ttgccgagcg tgaagcaatt gcacggcagg tgagggactc
     8341 cgcgtaccac attatcggat acaagggggc tacgtggttc gcggtggggt tggccctcgt
     8401 ccggattgcg ggcgcgattc ttcgcaatca gcgaagcgtg ttgacggttt ctgccctgct
     8461 ccacggtgaa ttcggcctga gcgatgtgtg tatcggggtg ccgtgcattg tatccgcacg
     8521 cggcatcgag cgggttgtgg agagcgtcct ttcggagtct gaactggccg cgctgcattc
     8581 ctccgcacgt acgctgaaga aggccgtgga gcagttgcac gcgggcgcgt gacgttctgc
     8641 gatttgtgat gttcacccgc cgcacaccgt tgcggcccct gttcaaccct ccgatggcga
     8701 ggtctgcgta tttccgtctc cgcaaaatga atgctcatcg cgcagaaact ctgaagcatc
     8761 ttcaccgctt gaacgacggc cacgcgtgca tttgcgttcc gagggaagac gccggctgat
     8821 ttcttggtct ggtcgcgtcg cgcgtgtctt gcgttgcgcg cgctgatcgg cctagaatca
     8881 ttcgaagaag cggcgaaagg gttcacaacc cgcgggtcac ctctggaccg tttgagagtg
     8941 cggacgggaa tctcggggaa aaatgaatcc tgcaggcgga atctgatttc gatgtctcct
     9001 gtgttcgtcg gtggtgtcat ccgggatagc aatggagaac caggaacacg ttgtcatcgc
     9061 aggggccggt cccgctggcc ttgcatcggc gataacgctt gcccgtgcag ggttgagcgt
     9121 ggaagtacgc gagttgcgcg gtgacgtagg cggccatttc cggggaaatc tccagttgct
     9181 taccaactac ccggatttgg aggacgcacg gaccgagctc tgcagcctgc tgggttcggt
     9241 tcccgtgtcg ctctggccgc agcgatcggc gcaactgttt ggcccatcag ggaaatgcgt
     9301 gagcggacgg agctccgaac cgttcgggta cctgcttcac cgggggcccg atccgaccgc
     9361 actggatggc gccttgaaac gggccgccga ggaagcgggg gttcagttca ggttccacac
     9421 gagggcgcgt cctgaagagg tcgatatcgt tgccacgggg ggagcagccg cgcagggtgt
     9481 ggcccgcgaa tggttcggga gaaccgaact tccggacggg ttccacgcgg tgtttgacaa
     9541 ccggatttgt ccggggggat tcggatatct acttgtggcc gaaggatata ccgtcgttgg
     9601 cgcggcagtc gtcagacacc gggagcgcgc acaggaggcg tttcacaaga cggtgcgttt
     9661 ttttcgggag cgcgcaggtg taccccgaat cgaacatccc acattgtccg gatcactcga
     9721 tctttttctc cccgatcctg aacgtactaa catgcgcccg ttgcacgtgg gtgaagcggg
     9781 agggtttgca gaccatcttt tcgggttcgg gatcaggctt gccctgcaat ctggtgttct
     9841 gg
//
LOCUS       DAOTNG010000087         9808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6398_length_9808_cov_5.612324, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000087 DAOTNG010000000
VERSION     DAOTNG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6398_length_9808_cov_5.612324"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="P5179_05725"
     CDS             complement(<1..1593)
                     /locus_tag="P5179_05725"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS94751.1"
                     /translation="MEQKDKDAHKRVEEWTARAFDAAANTPPFSFVYGGERVLLSENR
                     WEPELLTATTAGHAREFSCVFRDRRTGLVCTCEFNQFLDFPAVEWLLHFENAGTNATP
                     IISDILPLDTLFAGAGASTIRHANGSLCRNDDFAPHETALKPGETFRIGTTGGRSSNG
                     ALPFFSLHTDKERVIGGIGWTGNWRCTFAANETGGVRVSAGMQKTHLSLLPGEKIRSP
                     RILLLFWNGEPIDGQNALRRFLLAHHTPLAAGNPPEVPLSFVGWGENRSERQIAKARW
                     FALQEIPLDNYWIDAGWHGDAEFCDKATVFNTNWGAHVGNWWPNRSNYPEGMAPIGKV
                     TRELGMKFTLWFEPERVFKGTFLTRHHPEWLSGPIGDNWLFDLGNREARAGLVDIISS
                     VITEGGITVYRQDFNMDPEPFWNSMDTPERVGMAEIRHIEGLYAFWDELMDRHPGLLI
                     DNCSSGGRRIDLETISRSIPLWRSDYQCYPDFDPIGMQGQTHGLSLWVPLSAGCCDRP
                     DTYAFRSTLIPGATLTTTVARPD"
     gene            1780..2190
                     /locus_tag="P5179_05730"
     CDS             1780..2190
                     /locus_tag="P5179_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS94752.1"
                     /translation="MVTGIGHAAYGVSDLNASLRFYCGQLGLRKAFELHRDDGTLWIV
                     YLYAGNRSFIELFPESEVPPQEGGSYRHLCLIVDDMAATLRAMKIAGLEPLNPPSVGK
                     DGNTQAWVRDPDGNPIELMQIAPDSDQARAITSG"
     gene            2305..3783
                     /locus_tag="P5179_05735"
     CDS             2305..3783
                     /locus_tag="P5179_05735"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRS94753.1"
                     /translation="MVVRWAVAALIGLWNLSAAHAAERLRVVVHELDANGVSPQVARV
                     VSEQLRSKLIETRRFVVPEREKMEAVVAEQSVSLSLLACSSQECAIELGRILQANKMV
                     VGSVSLLNQTYSINTRFLDLETGAAEFSAEEKCQTADDLFLAAERLAARIAASVPQRG
                     TVTAVSGENIIIDLGALDGVSTGMQFRVLRRVERVQGYWEEEQVALAVVTSVQPTWSR
                     IEPQRAGLNFMRPVSIQQNDIVVGVQSITVDELPRYAFLSVYSRPVGAEVYVDNLFQG
                     RAPESGLEVRLTPGKHTVRVSAPAHRTEERSIELRPDERRSFNATLQPRLRRIPFRMQ
                     VLTGGYVQDRPANAAFRSRLENERMNGARVGIGRVYSVFMSEAGGSWTHANMPADRSF
                     GLNEVHNLSLHAQGGISPRLGWVLPYVALGYEFSRFYFNDQNVFEGQQSLGDAGKVGN
                     SGWYWGGGAFLGHWFHVGFRQTIGRPETDFRSLTIGVNLAGF"
     gene            3783..4958
                     /locus_tag="P5179_05740"
     CDS             3783..4958
                     /locus_tag="P5179_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRS94754.1"
                     /translation="MGLYRWTSGVGAVVAMALVACGCSDDFRGTLLAPLMFSEAANDS
                     SGLYREPNDTPEQAILIRTDGTRTRQTIHLPSDVDYFRFLAAAGAQYLVNVETETGGS
                     LQLEITDERGVVQPVTVVNAPGANRSTRSWTTAESGYYFVRVSAPVAANSVVGTYSVW
                     IVVGEDGFEPDNTISQAKEIAINGTWQQHTLPGMADKDYMSFSAWANFHYEFLFDGAS
                     GEHVSFAVVDGSGNSVSSRGAPSLVRFDAPSDGTYFVEVIPVDLGALGTYRVSVFSTD
                     PWEPDSTLALATPISTTGEPQRHSFSRVSDQDYLKFQAAAGVTYIIRTDSLGYGVDTQ
                     IEIDSASGTALVSDDNSGGGNASMLVWTAPVTGAFFVRVRSTTGGFPGKYVVSVKSN"
     gene            5064..5699
                     /locus_tag="P5179_05745"
     CDS             5064..5699
                     /locus_tag="P5179_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94755.1"
                     /translation="MRRSIVFPLVAAGAMFVLSACGSKPPEVGQQFIIERVPDERPRW
                     ITRPPADRDDKLFFVGLKTHASSLENGNTDARQNAIQKIVEYVGGTGMVDYTKARVES
                     GLTDEGQAGNYVEDGFRFLAESIAQGMREDENYYERVKEWQPTGWRYFYNYHVLVSIP
                     KNSLADAARQAFERQAAAARARNDARAEAFANKLREQLTPGMTPQSTVGGQ"
     gene            5786..7324
                     /locus_tag="P5179_05750"
     CDS             5786..7324
                     /locus_tag="P5179_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94756.1"
                     /translation="MKRHLPFPCVRLLVGVSAVLLIASCGPRTVNTMPRGERRLVEAS
                     ADRVPDWITREPASDAEYHYFRGIRTDAPSLEAGETDARQNALASIVQFLGLRVTVDY
                     QRLRTEEQTRIADAIRSVGGADLFGTRLSEMFYRRWRVIDGGEVHDKYDVYLIVRFPK
                     ESVDRIKRNQQERLQAISRLVGNPAGEPSDPSGFYAQISSISQALRALTDLNQSVLIT
                     TETEGQAQQLKALATTKLLRLVGSARLSLTPPAARIATGRHNPPLVFSCLATNDRGVP
                     IPNLPLRLSVGDSSQVIWSNASGVAEVTRSEIHSTNGRFLFSAQLDLPVDVRSLPDIA
                     RTVPSVSSSLEVVPRTETIRLLVVVDEWHNRRSPSRRTAEERLIAALRERGFTVVSAA
                     EFPRNEIGNPWSNQNDAFDLARKSHSDFLVWGSVETGEATLVPYSTSLYATSATAQIR
                     AIDAVSQNVMATVVLPDGVMRDTRGFGNTPERAAEEAVTLARRDFPNGYAYLAEQLER
                     ALTQ"
     gene            complement(7387..8088)
                     /locus_tag="P5179_05755"
     CDS             complement(7387..8088)
                     /locus_tag="P5179_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRS94757.1"
                     /translation="MRLLSSLFRWFCGWIVFVMGAPWILLCGLVLAPERFFRVVQVWS
                     RALLRVLSIRVVLHGEFPRRETGAFVAVAPHVNLFDPFVMASILPRPIAGVELDKHFR
                     WPLYGRIIARVGNIPLSHTTPQRTRESLRLVEERLKKGQPLVVFPEGHRTRTGRRGEF
                     GSWIFRVAARTGTPVVPIAFRGAWERNHVGSLLITPGTWEVEILPPIIPAGGDRAAAE
                     ALKKAVEEAIDRKTQ"
     gene            complement(8130..9044)
                     /locus_tag="P5179_05760"
     CDS             complement(8130..9044)
                     /locus_tag="P5179_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRS94758.1"
                     /translation="MADEEKRWLAIVNPTAAGGRCGERIGDAVDELRWRGLAVTTRFT
                     TKPGDASTLAAEGIAEGYTRIMAVGGDGTVNEVVNGIAKTRADGTITLATLPLGTSNS
                     FLRDFNQQKLPNAIARITAGTAPPCDLVRCRILVDGVPTERWFINNVIVGFGANVGDT
                     MNRRLKFMGKHGYSVGVFIEVARLKTPVMKITVDGVSWSGPVTMVNIGNSQFTGGVMH
                     ISPGALVDDGLFDVVTIGKLSRTGLLLAFPRIFNGTILSHPKITHARGKRLTLETDNP
                     LPVLFDGDISGTTPLEAELVPGAILVIR"
     gene            9516..>9808
                     /locus_tag="P5179_05765"
     CDS             9516..>9808
                     /locus_tag="P5179_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94759.1"
                     /translation="MEDDALRKRVLMVISVVMLFSGLDATSALDRPLWATCTFGSPRV
                     EVGLALTGTTNVSTSNGKTYVQQNTTSNGVLSFSVAPAHLDGVAHGVFVIVDA"
BASE COUNT         1983 a         2856 c         2965 g         2004 t
ORIGIN      
        1 atcggggcgg gcgacggtag tagtcagtgt ggcccccgga atgagcgtgc tccggaatgc
       61 atacgtgtca ggcctgtcgc aacaccccgc actcagggga acccacagag aaaggccgtg
      121 tgtctgcccc tgcatcccga tcggatcaaa atcgggatag cactgataat cgcttctcca
      181 gagcgggata ctccgggaga ttgtttcgag gtcgatgcgt ctcccgccgc tggagcaatt
      241 gtcgatcagc aaccctggat ggcggtccat gagctcgtcc cagaatgcgt acaagccctc
      301 aatatgccgg atttcagcca ttccgactcg ttcaggcgta tccatggaat tccagaacgg
      361 ttcgggatcc atgttgaaat cctgccggta gacggttatc ccgccttcgg taatcacgga
      421 tgagatgatg tcgaccagtc ctgcgcgcgc ttcgcggttt cccagatcaa acagccagtt
      481 gtctccaatg ggtccggaaa gccactcagg gtggtgccgg gtaagaaagg tccccttgaa
      541 cacgcgctcc ggctcaaacc aaagtgtgaa tttcattccg agttcgcggg ttaccttccc
      601 gatcggtgcc atcccctcgg ggtaattgct cctgttcggc caccagtttc cgacgtgcgc
      661 gccccagttg gtgttgaaca cggtggcctt gtcgcagaat tctgcatcgc cgtgccatcc
      721 cgcgtcgatc cagtagttgt cgagagggat ttcctgtaac gcaaaccagc gcgcctttgc
      781 gatttgccgc tcgctgcggt tttcgcccca ccccacaaac gagagaggca cttccggcgg
      841 gtttccagcc gcgagcggcg tatggtgggc gagaaggaag cgtctgagag cattctgccc
      901 gtcaatgggt tctccattcc agaagagcaa caaaatgcgg ggtgatcgaa tcttctcgcc
      961 gggcagaagc gacagatggg tcttctgcat tcccgccgac acgcgcactc cgccggtttc
     1021 gttcgcggcg aacgtgcacc gccagtttcc tgtccacccg atgccgccga tcacccgctc
     1081 cttgtctgtg tgcaggctga agaacggcaa agccccgttg gaggaacgcc ctcccgtcgt
     1141 gccaatgcgg aatgtctctc cgggtttcag agccgtttcg tgcggggcga aatcgtcatt
     1201 gcggcagagg cttccgttgg catggcgaat cgttgatgct cccgcgccgg caaacagagt
     1261 gtccagggga agtatgtcgc tgatgattgg agtggcgttg gtgcctgcgt tttcaaagtg
     1321 gaggagccat tccaccgcag ggaaatcgag gaactggttg aattcgcacg tgcacaccag
     1381 tcctgttcga cggtctcgga aaacgcatga gaattcccga gcatgcccgg cagtagttgc
     1441 ggtcagcagc tcgggttccc agcggttttc cgaaagcaga acgcgttcgc cgccgtacac
     1501 aaaagaaaac ggaggggtgt tcgcagcggc gtcaaaggcc cgtgcggtcc attcttcaac
     1561 gcgtttatga gcgtctttgt ctttctgttc catggtcttc gtccttcgga aatcaacgtg
     1621 ccgttccgtc gcccggtgaa gagaatgtta ctctacccgg cggttcgaac ccacgccctc
     1681 ccccgttccg tttttggcgt ccagggggta ggttcaatgg ggctttgtcg cgattcattt
     1741 tttccttcgc gaacttatcc acggaaagga gtcaactcaa tggttaccgg aatcggtcac
     1801 gctgcatacg gggtcagcga cctgaacgcg agtctgcgtt tttactgcgg gcaactcggt
     1861 ctgaggaagg ccttcgaact gcatcgtgat gatggcactc tgtggattgt atacctctac
     1921 gccggtaatc ggtcgttcat cgaacttttc ccggaatcgg aggtgcctcc gcaggaaggg
     1981 gggagctacc gacacctttg tttgatcgta gacgacatgg cggcaacact gcgtgcgatg
     2041 aaaatcgccg gtcttgagcc tctgaacccg ccatccgtgg gaaaggatgg gaatacgcag
     2101 gcatgggtgc gcgatcccga cggcaacccc atcgagctca tgcagatcgc acctgattcg
     2161 gaccaggccc gtgcgataac gtcggggtaa tgtgagctct tttggcacgg catctgcagg
     2221 gggctgtcat cagtcggaac tcggcgtgcg cccgatgacg agttcatcgg gacacagaac
     2281 gaaacctgga gatcaggatt ggtgatggtt gtccgttggg ccgtggcggc cctcattggc
     2341 ctgtggaacc tttccgccgc gcacgcggct gagcgactgc gcgtcgtggt ccatgaactg
     2401 gacgcgaacg gcgtatctcc gcaggtagcg cgcgtcgtat ccgaacagct tcgatccaaa
     2461 ctgattgaga cacgacggtt tgttgttcct gagcgcgaaa agatggaggc tgtcgttgcc
     2521 gagcaatcag tctctctgag cctcctcgcc tgttccagcc aagagtgcgc gattgagctg
     2581 gggcgcatcc ttcaggcgaa caaaatggtt gtcgggtcgg tctccctcct taaccagacg
     2641 tacagcatca acacgcgatt cctggacctg gaaacgggtg cggcggagtt ttccgccgag
     2701 gaaaaatgcc agaccgcgga cgatctgttt ctggcagcgg aacggctggc cgcgcgaatt
     2761 gccgcttccg ttccgcaacg aggtaccgtt acggcagtga gcggggagaa tatcattatt
     2821 gatttgggcg ccctcgacgg agtatcaaca gggatgcaat tccgcgtcct gcggagggtg
     2881 gagcgggtac agggttactg ggaagaagaa caggtggccc tggccgtggt aacatccgtt
     2941 caacccacgt ggtcgcggat agagccgcag cgcgccggat tgaatttcat gcgtccggtt
     3001 tccatccagc agaacgacat cgttgtcggc gttcaatcga tcactgtcga cgaattgccg
     3061 cgatacgcgt tcctgtcggt gtactcacgc ccggtggggg ccgaggtgta tgtggacaat
     3121 cttttccagg ggcgtgcgcc ggaatcggga ctcgaagttc ggctgacgcc ggggaaacac
     3181 accgttcgtg tgagcgcacc ggcccacagg accgaggaac gctccataga actgcgcccc
     3241 gacgagcgca gatccttcaa cgcaacgctc cagccgcgtc tgaggcgcat cccgttcaga
     3301 atgcaggtcc tcaccggcgg gtacgtgcag gaccgaccgg cgaacgccgc gttcagaagc
     3361 agactggaaa acgagcgtat gaacggcgca cgggtgggta tcggcagggt atactcggta
     3421 ttcatgagtg aggccggcgg aagctggacc cacgcgaata tgcctgctga ccgcagcttc
     3481 ggtctcaacg aagtccacaa cctctcgctg catgctcagg gcggaatctc gccccggctg
     3541 ggctgggtcc tcccgtatgt cgcgttaggg tatgagtttt cccggttcta tttcaacgac
     3601 cagaacgtgt tcgaggggca acaaagtctt ggtgatgccg gaaaggtggg aaacagcggc
     3661 tggtactggg gcggaggagc attccttggg cactggttcc atgtggggtt ccgccagacc
     3721 atcggcaggc ccgaaacaga tttccggtcg ttgaccattg gcgttaacct ggcgggtttc
     3781 tgatggggct gtaccgctgg acatcgggcg tcggtgctgt cgtcgcgatg gctcttgtcg
     3841 catgtggctg ctctgacgac ttccggggca ctctgcttgc gccactgatg ttttccgagg
     3901 cggccaacga ctcctcgggc ctgtatcgcg aaccaaacga caccccggaa caggcgatac
     3961 tgatcaggac ggacggaacc agaactcggc agacgatcca tcttcccagt gacgtggact
     4021 acttccggtt cctcgccgct gccggggctc agtacctggt gaacgtggag acggaaaccg
     4081 ggggctcact tcagttggag atcacggacg aacgcggagt tgtgcaaccg gtgacagtgg
     4141 tgaatgcacc cggcgcgaac aggagcacca gaagctggac aactgcggag tcagggtact
     4201 atttcgtacg ggtgagcgcg cccgtcgccg cgaactcggt tgtcggaacg tacagcgttt
     4261 ggatagtcgt cggggaggac ggttttgagc cggacaacac gatctcgcag gcgaaggaaa
     4321 tcgcgataaa cggcacctgg cagcaacaca ccctcccggg gatggcggac aaggactaca
     4381 tgagcttctc ggcctgggcg aactttcatt acgaattcct gttcgacggg gcttccggcg
     4441 agcatgtgtc gttcgctgtg gtggatggtt cgggaaattc ggtctcgtcg cgtggtgcgc
     4501 cttctctggt tcggtttgat gcaccgtcgg acgggacgta tttcgtggaa gtcattccgg
     4561 tagatctcgg tgccctgggt acataccggg tttccgtctt ctccacggat ccgtgggaac
     4621 cggacagcac gctcgccctt gcgacaccga tctcgactac aggggaaccg caacggcata
     4681 gcttttcccg tgtatccgat caggattacc tgaaatttca agccgccgcc ggagttacct
     4741 acattatcag aacggatagt ctcggttacg gtgttgacac ccagattgaa atagacagcg
     4801 catccggcac ggcgttagtt tctgacgaca acagcggggg aggaaacgcg tcgatgctgg
     4861 tgtggacggc acccgttacc ggggctttct tcgttcgcgt tcggagcacc accgggggat
     4921 tccctggcaa atacgtggtg tcggtgaagt cgaattgata tggcacacac gttgcagtct
     4981 ggtgaacgtg aatgttctgt caaagagcgg ttttcatcca gagcatgtcc gagtaccaca
     5041 ataccgaacc aggaggttcc gccatgcggc gatcaatcgt gtttcctctc gttgcagccg
     5101 gcgcgatgtt cgtgctgagc gcgtgtggct cgaaaccacc ggaggtcggg cagcagttta
     5161 tcatcgaacg cgttcccgat gaacgtccgc gctggataac gcggcctccg gcggatcgtg
     5221 atgacaagct cttcttcgtc ggcctcaaaa cccacgcatc aagcctggaa aacggcaaca
     5281 ccgacgcccg gcagaacgcc attcagaaaa tcgtggaata cgtgggtggt accgggatgg
     5341 tggactacac caaggctcgc gttgagtccg ggcttaccga cgaagggcaa gccggcaact
     5401 acgtggaaga cggcttccgc ttccttgccg aatccattgc ccaaggcatg cgcgaagacg
     5461 agaattacta cgaacgagtg aaggaatggc agccgaccgg ctggcggtac ttctacaact
     5521 atcacgtgct cgtcagcatt ccaaagaact cgctggccga cgccgcgcgc caggcgtttg
     5581 aacgtcaggc ggctgcagcg cgcgcccgga atgacgctcg cgcggaagcg tttgccaaca
     5641 aactccggga acaactcact cccgggatga ccccgcagtc cacggtcggt ggtcagtaaa
     5701 ttcagctcaa acggcgtgcg agagcaaacg gggaagccgg gtcaaaccgg tttcccccct
     5761 tttccaaggc tggcgaatac ttgccgtgaa acgccacttg ccctttccct gcgtccgact
     5821 tctcgtcggt gtgagcgcgg ttcttctcat tgcctcgtgc ggccccagaa ccgtgaacac
     5881 gatgccgcgg ggagagcgcc gccttgttga agcatcggcg gaccgagtcc ccgactggat
     5941 tacgcgggaa ccggccagcg acgccgaata ccattatttc cggggcatcc ggacagacgc
     6001 tcccagtctc gaagcaggtg aaacggatgc ccgccagaac gcgctggcca gcatcgttca
     6061 attcctcggc ctccgggtga ccgttgatta ccagcggttg cgaactgaag agcagacgcg
     6121 cattgcagac gcgattcggt ccgttggcgg tgcggatctg ttcgggacgc ggctctcgga
     6181 aatgttctac cggcgctggc gggttattga cggcggggaa gtgcacgaca agtacgacgt
     6241 gtatctgatt gtgcgtttcc cgaaagaatc tgtcgaccga atcaaaagaa accaacagga
     6301 gcgacttcag gcgatttcgc gtctcgtggg aaaccctgca ggtgaaccgt ctgacccgtc
     6361 aggtttctac gcgcagattt cgtcgatttc gcaggctctc cgcgcactga cggacctcaa
     6421 tcagtcggtg ctcattacga cggaaacgga gggacaggcc cagcagctca aagcgctggc
     6481 gacgacgaaa ctcctccggc tcgtcggctc cgcgaggctt tctctgacac cgcccgccgc
     6541 acgcatcgca acgggcaggc ataacccgcc tctggtgttc tcgtgtctgg caacaaacga
     6601 ccggggcgtg ccgataccaa accttcccct caggctctcg gtcggagatt cgtctcaggt
     6661 catatggtcc aacgcgtcgg gggttgccga ggttacccgt tcagagatcc attccaccaa
     6721 tgggcggttc cttttctcgg cacaactgga ccttccggtt gacgttagat cgcttcccga
     6781 catcgcgcgc actgttcctt cggtatcgtc ttccctcgag gtagttccgc gtaccgaaac
     6841 gataaggttg ctcgttgtgg tggacgagtg gcacaatcgc cggtcaccgt cccggcggac
     6901 ggccgaggag agactcatcg ccgcgttgcg ggagcgcggt tttacggtgg tcagcgcggc
     6961 cgagttcccg agaaacgaaa tcgggaaccc ctggagcaat cagaacgacg cgtttgacct
     7021 tgcccggaag agccattctg attttctcgt ctgggggtcg gtggaaacgg gcgaggcaac
     7081 gctcgtgccc tattccacca gcctgtacgc aacatccgcg actgcgcaga tcagggcgat
     7141 tgatgcggtg tcgcagaacg tcatggctac ggtggttctg ccggatggag tgatgcggga
     7201 taccagaggt ttcggcaata cgcccgagcg agcggcggag gaggccgtca cacttgcgcg
     7261 gcgcgatttt cccaacgggt acgcctacct cgcggaacag ctggagaggg cgctgaccca
     7321 atagattgcg ccggaaatta ccggacgatt ccccgtgtgg ttcctctccc tccatccgtg
     7381 aatccgtcac tgcgtttttc tgtcgatcgc ctcctcaaca gcctttttga gagcttccgc
     7441 ggctgctctg tcgccacctg ctggaatgat gggcggaagg atctctactt cccacgttcc
     7501 gggggtgatg agaagcgaac cgacatggtt ccgctcccat gcccctcgaa aggcgatcgg
     7561 aaccaccgga gtccccgttc gcgcggcaac gcgaaaaatc caggatccga attcaccacg
     7621 ccggcccgtg cgcgttcgat gaccttccgg gaagacgacc agtggttggc cctttttgag
     7681 acgctcttcc accagccgga gggactcccg tgttctttgc ggggtcgtat gactcaaagg
     7741 gatattgccc acccgagcga tgattctgcc gtaaagaggc caccggaaat gtttgtcgag
     7801 ctccaccccc gcaatcgggc gaggcagaat cgaagccatg acgaaagggt cgaaaaggtt
     7861 cacgtggggc gccactgcga cgaatgcgcc ggtctcgcgc cgagggaatt ccccgtgcag
     7921 gactacgcgg atgctgagca ctcgcaatag agccctgctc cacacctgta ccacccgaaa
     7981 gaaccgctcg ggagcgagta cgagcccgca gagcagaatc cacggcgcac ccatcacgaa
     8041 gacaatccac ccgcagaacc aacgaaaaag agaggaaagg agtctcatgt ctgaatcctg
     8101 gagcaattca gctcccgttc ccatcggatt cagcggataa ccagaatcgc cccgggaaca
     8161 agctcggctt ccagcggcgt tgtgccggag atgtctccgt cgaaaagcac gggcagggga
     8221 ttgtcagtct cgagagtaag cctcttgcct cttgcatggg tgattttggg gtgtgaaagg
     8281 atggtgccat tgaatatccg tggaaacgcc aacaggaggc cggtgcgcga aagtttcccg
     8341 atggtgacga catcaaaaag gccgtcgtcc accagtgctc caggcgaaat atgcatcacg
     8401 ccgccggtga actgtgaatt cccgatattc accatggtga cagggcccga ccacgaaact
     8461 ccgtccacgg ttatcttcat gaccggagtc ttcagtcgtg cgacctcgat gaacacccct
     8521 acggagtacc cgtgtttgcc catgaacttc agccggcggt tcatcgtatc gcccacgttg
     8581 gccccaaaac ccacaatcac attgttgatg aaccatcgtt ctgttggaac accgtccaca
     8641 aggatgcgac accggacaag atcacacgga ggcgcagtgc ctgcggtaat tcgcgcgatt
     8701 gcgttcggaa gcttctgctg attgaaatcg cggaggaagg agttcgatgt acccagggga
     8761 agcgtcgcga gggttatggt tccatccgca cgcgtcttcg cgatcccgtt gacgacctcg
     8821 ttcacggtgc catcaccgcc gaccgccatg atacgcgtgt acccttcagc gattccctcg
     8881 gctgccagcg tgctcgcgtc gccgggtttc gtcgtgaagc gtgtggtcac tgccaggcca
     8941 cgccacctga gttcgtcaac cgcgtcccct atccgttctc cgcacctgcc tccggccgcg
     9001 gtagggttga cgattgcgag ccagcgtttt tcttcatccg ccatatgcgc ttccatgcat
     9061 gggtacgacc gttgtcgcca cggtctgacg ggtctgcgcg acagacctgg cactcaagtc
     9121 ccacgaacgc gtggctttca aggttgttca agagtacaaa acggagctag atttaccaaa
     9181 cccgcgccaa aggcagttgc ttcgggacag ttggcgagac gccgttgcat ttcgaggaag
     9241 actcgtgctc gcgtcagaac gtgcgaccgg tagtgaattg acgcttcgta tcttggtggc
     9301 aacgggcgcc tgaaacggac gtgcctgaag cggacttcca actcgttgcc ggtctcgcgg
     9361 cagacaacgc gaatgcggtg ggtccggaat gcatgagacc cactcaggcg cgggaagatc
     9421 gcccggccgc cattccaaag acggaggcca cccggctccg attccacatt cagaacggcg
     9481 acctgttcgg ctgcaaggct tgcgaagagt gacccgtgga ggacgacgcg ttgcggaagc
     9541 gagtgctgat ggtaatctcg gtcgtcatgc tgttttccgg gctggacgca acctcggcac
     9601 tggaccgtcc tctctgggca acctgcacgt tcggctcgcc acgggttgaa gttgggttag
     9661 cgctcacagg aacgaccaat gtctccacat ccaacggaaa gacctacgta cagcagaaca
     9721 caaccagcaa cggcgtgctg tcgttttcgg ttgccccggc ccacctcgat ggtgtagcac
     9781 acggtgtgtt cgtcattgtt gacgcgga
//
LOCUS       DAOTNG010000088         9807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6399_length_9807_cov_5.361567, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000088 DAOTNG010000000
VERSION     DAOTNG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_9807_cov_5.361567"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5179_05770"
     CDS             complement(<1..429)
                     /locus_tag="P5179_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94760.1"
                     /translation="MSLRRFWQVLVVAVVAAESVFSATTPEREHTRAWWVWFVDKGPE
                     QRADDDSAPVFPAYLARVHGIAPVRTTTRWFNGASVLASEEQLAGIMELPFVRGVAPV
                     LPLRGERPLPADGLLAAERPARSGALDYGLSLTQNRMIGAD"
     gene            complement(440..1087)
                     /gene="lolA"
                     /locus_tag="P5179_05775"
     CDS             complement(440..1087)
                     /gene="lolA"
                     /locus_tag="P5179_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HRS94761.1"
                     /translation="MVHVRRWVLMAAGVLGVSVAAGAITGNEVLAKIRGTYDKVNSFQ
                     AAFEQTFEWKLAGTTQTMRGTFSMKKPNKFRIQTNVQTVVSNGRVVWSYSPATGQVII
                     TNYDPATMPLRPDNFLFVFPDERRTTYVRSERLSGEDHHVVDVTPRDSTLGISKMRVW
                     VDGRSWLAKKVQYTSVSGDVTTYLMNAIQTNTSIPDSVFTFTPPPGVDVIDFRNR"
     gene            1308..2936
                     /locus_tag="P5179_05780"
     CDS             1308..2936
                     /locus_tag="P5179_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRS94762.1"
                     /translation="MIGSERLLLSGNEAVALAMKSAGITLGTGYPGTPSTEILENFSA
                     RGGRAQWAPNEKVAFEVALGAAYGGARSVITMKHVGVNVAADPLFTAAYTGVPAALIL
                     ISADDPGMASSQNEQDNRHYAVAAGLPMIEPSDSQEAHDFFLHAVAVSERWHIPVLFR
                     MTTRVCHSKTIVNTGLRQELAAPAPPAYVRSIRERVMIPAYARPAHRKLRQKLAEIAE
                     SNETSPLNTRTSGVRTLGIISSGIAAMHAREVAPDASFLKLGTTYPLPMRIIRDFAES
                     VDRCVVVEEGDPYLADSIRAAGVPVHAKPDMYRFGELNVGRVKRILANDTSPEEPVPS
                     GKPPQLCKGCPHRVVFEAFRDLGCIVAGDIGCYTLGVLPPFEAMDTCVSMGASITVGL
                     GLRHVLPREQAVKVVSVIGDSTFAHSGLSGLVEMVYNPPATGHVVVIVDNGTTAMTGQ
                     QEHPGTGRTLDHEPTGKLVFEDIARAMGIKEVHVIDPLKEEGEFARLLAETLAKEQPA
                     VLITRRACLLAAGRIRAYEQRALQEKEACDADGK"
     gene            2920..3414
                     /locus_tag="P5179_05785"
     CDS             2920..3414
                     /locus_tag="P5179_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRS94763.1"
                     /translation="MPTGSDVVNVVIAGLGGQGVIKASDILADVAFRAGYDVKKSELH
                     GMSQRGGSVSSDVRFGKKVWSPMVPFGCADYLVVLSSDQLEVNRHVLKEGGVTLSAEL
                     VDVSRLASPRCINVALLGALSAHLEFPEELWNAAIRSGFPEKLHDMNITAFQFGRTCT
                     KTQQ"
     gene            3443..4777
                     /locus_tag="P5179_05790"
     CDS             3443..4777
                     /locus_tag="P5179_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRS94764.1"
                     /translation="MTKTAGHGLGGFNPVSAPDYLPVPLLRDLQLARLQQIVRRAYEN
                     VGHFRKQMDARDLNPDSIKSLQDISKLPFTVKNDLRDTYPFGLFASPMGDIVRLHASS
                     GTTGKPIVVAYTKDDVEVWTSAMMRAFAACGVGSGDIIQNAYGYGLFTGGLGAHYGGE
                     ALGATVIPISGGNTDRQIMVMKDFGVTAIACTPSYFLHLLERASELGVDFHDLRVRVG
                     IFGAEPWSETMRRRIEEMSGIKAYDIYGLSEIVGPGVGIECSCQCGLHIFEDHFYPEI
                     IDPVTNAPCAEGEEGELVLTTLSKQAMPMIRYRTRDITSFVEGPCACGRTLRRIKRIG
                     RRADDMFIIRGVNVFPSQIESALLAVEGTLPHYQIVLTREKGLDQMEVQVEVTAEVFS
                     DRIGALEALHTKLESELEHTLGIRAEVRLVEPHAIQRSEGKAKRVIDKRNLG"
     gene            4812..5243
                     /locus_tag="P5179_05795"
     CDS             4812..5243
                     /locus_tag="P5179_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRS94765.1"
                     /translation="MKIHQLSLFLENKPGQLTAPCRELADAGIDIRTLSLADTQQFGI
                     LRLIVSDWKRGYELLRDKGYVVKDTEVVAIEVPDRPGGLLEVLKVFEKSAVNIEYMYA
                     FTFGRGDKAVLIFRFDDPDAAIALLNSAGINVVGSVEVYNR"
     gene            5299..6627
                     /locus_tag="P5179_05800"
     CDS             5299..6627
                     /locus_tag="P5179_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRS94766.1"
                     /translation="MAEFLFENRIWDPAETLPRNELQALQVRRLRECVARLAHVPFYR
                     KAFAEGNVSADKIRSLDDLKRLPLTTKADLREHYPLGFLATPRSEIVRIHGSTGTTGK
                     PTFVAYTRDDLALWANLCARFLVAGGLRPEHMVHIAFGYGLFTGGFGLHYGAELVGAA
                     IVPAAAGNTERQIVLINDLQADVLVCTPSYALNIAEMMRAQGYTPDKIPLKFAHFGGE
                     PWTEDMRLEIERQMGLLAFNNYGLSEVIGPGVSGECVVRNGMHIQEDHFLVECIDPDT
                     LEPVPYGEPGELVLTALTKQAMPILRYRTRDIARLDPTPCPCGRTGIRMGRVIGRSDD
                     MLIIRGANVFPSQIEEALLRVEGTAPHYLIEVDRPGTLDEVTVRVELRPERFSDKMSQ
                     MHVLREDIERQIHIVAGIRANVELVAPQTLERFIGKAKRVVDHRKEKGLI"
     gene            6641..7825
                     /locus_tag="P5179_05805"
     CDS             6641..7825
                     /locus_tag="P5179_05805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS94767.1"
                     /translation="MGIAKTIATNLEQSSWIRRMFEEGIRLRAERGPEHVYDFTLGNP
                     DIEPPPEVIAVLERIVARNRPRSHAYMPNAGFPEVRAVIARKLARRTGLAYTADHILM
                     TAGAAGAINVVLKSLLDPGDEVIVLAPYFAEYRFYIENHGGVMVVVPTDDAFMPDTRR
                     IRAAITSRTKAILINTPNNPTGAVYGPGVLRSLNELIGEVNHPITIVSDEPYVALTFD
                     GVEPPETASLISRSVVTYSWSKALAIPGERIGYIAISPLLPEAADLFDACTFANRVLG
                     FVNASAIWQLVVAEAGDVRVDATPYQEKRDFLWDALTRMGYEIVKPGGAFYLFPKSPL
                     PDDVAFIRLLQKEGILAVPGSGFGTPGYFRLSLTVPKEMMERSLPGFERTLHAAEGNG
                     KS"
     gene            7934..9643
                     /locus_tag="P5179_05810"
     CDS             7934..9643
                     /locus_tag="P5179_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94768.1"
                     /translation="MSGHLKALSVVTSCLLLNTSLPLAVRAGVPDPRTTILVGVWGYP
                     PAPDDPADPFPRFTGSGTPDWFVANYRALKVNYYSYDSIKAAGIDMTMTPLGVNLQNE
                     GHCFATVRAANAARLFCLPLHPDVYGQRESDHLDVARVRRALSNPVWATSEDSSVIGW
                     YIWDEPQNDREVDKALRLDSLLRAQLTEIGPLAGKDWLTFLGWFVWNDSLRIARILRG
                     IGTTGAGGPAVIGHEAFRFMNPDTAENPHIYPMDWLNASLARFYRTVRDNAPGTPWWS
                     VTHVTDEFGMGGPCVWYPYGVIDEASLRMDVFTRLGWGAKGIVYLTYSHYIDAIVLPD
                     YENCRPTGRTRVYDGRYWGADWADRHHTRRYARFPGVFTSEWSSETMSYRPTGFAAIY
                     GHIRAVNADVARIRPYLGPAEIFHPVMSDPPAVGGPYPTRSFFVDGVSAWEPDSVMRP
                     FKSGWGERDKAAVLVTPMKNGRDHYLLIVNQNIRYREGTVFESIRTGEAGDSAFVTVR
                     INAELIGGGGGYVAKNLVTGSITKKLIPSGHFVTLFDTLDTGEFRMYQIMKDTSSHVG
                     RLR"
BASE COUNT         2082 a         2839 c         2948 g         1938 t
ORIGIN      
        1 atccgcgcct atcatccggt tctgcgtcaa cgaaagtcca taatcgagcg cgccgctgcg
       61 ggcaggccgc tccgcggcga ggagcccgtc agcgggcaac ggcctttcgc cgcgcaaggg
      121 gagaaccggg gcgaccccgc gtacgaatgg cagctccatg atcccggcaa gctgctcctc
      181 agacgccagg acacttgcgc cgttgaacca gcgcgttgtt gtgcgaacgg gcgctattcc
      241 gtgcactctg gcaaggtagg cggggaagac cggcgcactg tcgtcgtcgg cgcgttgttc
      301 aggacctttg tccacgaacc agacccacca ggctcgtgtg tgctcgcgtt ccggggtcgt
      361 cgcagagaac accgattccg cggcgacaac tgcgaccaca agaacctgcc agaagcgccg
      421 cagagacatg tagggaccgt caccggtttc ggaagtcgat cacgtcaaca ccgggcggtg
      481 gcgtgaatgt gaacacggaa tcgggaatcg aggtgttggt ctgaattgcg ttcatcaggt
      541 acgtcgtgac gtctccgctg acactcgtgt actgcacttt ttttgccagc cagctccgac
      601 catcaaccca cacgcgcatt ttgctgatgc ccagagtaga gtcacggggc gtcacgtcga
      661 caacgtgatg gtcctcgccg ctcagccgct cggagcgcac gtacgtggtg cgccgttcgt
      721 ccgggaacac aaacagaaag ttgtccggac ggagcggcat cgtcgccggg tcgtaattcg
      781 tgatgatgac ctgaccggtc gccggggagt agctccacac cactctcccg ttgctgacca
      841 ccgtctgaac gtttgtctgg atgcggaact tgttcggctt tttcatggag aaggtgccgc
      901 gcatagtctg cgtggtgccg gcaagcttcc actcaaaggt ctgctcaaat gcggcctgga
      961 aggaattgac tttgtcatag gtcccgcgga tttttgccag aacctcgttg cccgtaatcg
     1021 ctccggcggc aacagacaca ccgaggacac cggccgccat cagtacccaa cgccgtacgt
     1081 gaaccatatc ggaacctttc gcaattgtcg tgtacccaaa agtgccaccg cgctctcaga
     1141 atgtcaatgc aaacggcgtg ccccgcaaag aagacccacc catcggagtg ttgcaccgtt
     1201 tggcggcgca aacgtattct ctgaaacacg tgtgtgggcg tcagagcgca tatccgaccg
     1261 gtttcgagga ggcgtgacgc cgcgcttcac ctattcgcgg ggcaggcatg attggttccg
     1321 aacgtctgtt gttaagtgga aacgaagcag tggcactggc aatgaagtcc gccggaatta
     1381 ctctcgggac cggttacccg ggtacaccgt ctacggagat tctggagaac ttctcggccc
     1441 gcggtggccg tgcgcagtgg gcaccgaacg agaaggtggc cttcgaggtt gccctgggag
     1501 cggcctacgg aggcgctcgt tcggttataa caatgaaaca cgtcggggtg aacgtcgcgg
     1561 cagaccctct gttcaccgcg gcatacaccg gggttcccgc cgcgctcatt ctgatatccg
     1621 cggacgaccc cggcatggcg tccagtcaga atgagcagga caaccgccac tacgccgtcg
     1681 ctgccgggtt accgatgatc gaaccgtcgg actcgcagga agcacacgat ttctttctcc
     1741 acgccgtggc tgtttcggag cgatggcaca tccccgtgct gtttcgcatg acaacgcgcg
     1801 tgtgccactc gaaaacgatc gtcaacaccg gattgaggca ggaactggcg gccccggcgc
     1861 cgcccgcgta cgtccgcagt atcagggagc gtgtcatgat accggcgtac gcgcggcccg
     1921 ctcaccggaa actcaggcag aagcttgcgg agatcgcgga atcgaacgaa acttcgccac
     1981 tgaacacgcg aacgtccggc gttcgaacgc tcggtatcat ttcgtcggga atcgcggcga
     2041 tgcacgccag agaagtggca ccggatgcgt cattcctgaa actcggaacc acctatccgc
     2101 ttcccatgag aatcatcagg gattttgcgg agtctgtgga caggtgtgtt gtggtggaag
     2161 agggtgatcc ttacctcgcc gattccataa gggctgccgg ggtgcctgtc cacgcgaaac
     2221 cggacatgta ccggtttggc gaactcaacg tcgggcgtgt caaacgcatc cttgccaacg
     2281 acaccagccc cgaagaaccc gttccctccg ggaaaccacc gcaactgtgc aagggctgtc
     2341 cccatcgggt ggttttcgag gcgtttcggg acctgggctg cattgtggcc ggagatatcg
     2401 ggtgctacac gcttggtgtt cttccgccgt tcgaggcaat ggatacgtgc gtttccatgg
     2461 gcgccagcat tacggtggga cttggtctgc gtcacgtgct cccgcgtgaa caggcggtga
     2521 aagtggtaag tgttatcggg gacagcacgt ttgctcacag cgggctgagc gggttggtgg
     2581 aaatggtcta caacccgccg gcgacaggac acgtggtcgt gatcgtggac aacggcacaa
     2641 cagcgatgac cggtcaacag gagcacccgg gaacaggacg aacactcgac cacgagccga
     2701 ccgggaaact ggtgtttgag gacattgccc gtgcaatggg aatcaaggag gttcatgtca
     2761 ttgacccgtt gaaggaagag ggggaattcg cgcggcttct ggcggaaacg cttgccaagg
     2821 agcagccggc cgttctgatc acccgcaggg cgtgcctgct tgcggcgggc agaatccgcg
     2881 cgtacgaaca gcgcgctctg caggaaaagg aggcgtgcga tgccgacggg aagtgacgtt
     2941 gtgaacgtgg tgatcgccgg cctcggcgga caaggcgtga tcaaagcatc tgatatcctc
     3001 gcggatgtcg cgtttcgcgc cgggtatgat gtgaagaaga gcgaactcca cggaatgagc
     3061 cagaggggtg gttctgtttc cagcgacgtt cgtttcggaa agaaagtgtg gagcccgatg
     3121 gttccgttcg ggtgcgcgga ttaccttgtg gtactctcgt cggatcaact ggaagtcaac
     3181 cgtcacgtgc tgaaagaggg tggcgtaacg ctgtcggcgg agctggtgga cgtgtctcgc
     3241 ctggcgagtc cgcggtgcat caacgtcgct cttctcggag cattgagcgc gcacctcgag
     3301 ttcccggagg aactctggaa cgcggcgatt cgctcaggat tcccggagaa actgcacgat
     3361 atgaacataa cggcctttca attcgggcga acatgtacaa agacgcagca gtaaacgcgc
     3421 gttcacatcg ggggagatca tcatgacgaa gacggctggc cacggtttgg gcgggttcaa
     3481 tcctgtcagc gctcctgatt acctgcctgt gccgctcctg cgtgacctgc agcttgcacg
     3541 gcttcagcag atcgtccggc gggcgtatga gaatgtaggc cacttccgga aacagatgga
     3601 cgcacgggat ctcaacccgg acagcatcaa atcccttcag gacatcagca aactgccgtt
     3661 tacggtcaaa aatgacctgc gcgacaccta cccgttcggg ctgttcgcca gccccatggg
     3721 cgacatcgta cggcttcacg cctccagcgg gaccacaggc aaaccgatag tcgtcgcgta
     3781 tacaaaggac gacgtcgaag tctggacaag tgcgatgatg cgcgcgtttg cggcgtgcgg
     3841 agtcggcagc ggggatatca tccagaacgc gtacggatac gggcttttca ccggcggact
     3901 cggtgcgcac tacggaggcg aagcgctcgg cgcgacggtg atcccgattt caggcggaaa
     3961 cacagaccgg cagatcatgg taatgaagga tttcggggtc acggcaatcg cctgtacgcc
     4021 aagctatttc cttcacctgc tcgaacgcgc atccgaactc ggtgtggatt tccacgatct
     4081 ccgtgttcgt gtcgggatct tcggcgcgga accgtggagc gaaacgatgc ggcggcgcat
     4141 agaggaaatg agcggcatca aggcgtacga catctacggc ctctcagaaa tcgtcggacc
     4201 tggagtcggg atcgagtgtt cctgtcagtg cggattgcac atcttcgagg atcacttcta
     4261 ccccgaaatc attgacccgg tcactaacgc gccgtgcgct gaaggagaag agggcgagct
     4321 cgtccttacg accctcagca aacaggccat gccgatgatc cggtaccgca cgcgggatat
     4381 tacctcgttt gtcgagggcc cgtgcgcgtg cggacgcaca ctgcgtcgaa tcaaacgaat
     4441 cgggcgccgt gcagacgaca tgttcattat tcgtggcgtg aatgtcttcc cgtcccagat
     4501 cgagtcggct cttctcgccg tggaaggcac gcttccacat taccagatcg ttctgacgcg
     4561 cgaaaaaggg ctggaccaga tggaggttca ggtggaagtc accgccgagg tgttcagtga
     4621 ccgtatcggc gcactggaag cccttcacac gaaactggaa tcggagttgg aacatacgct
     4681 cgggatcaga gcggaggtac gcctcgttga accgcatgca atccagcgaa gcgaaggcaa
     4741 agcgaaacgc gtgatcgaca aacggaatct cggatgatcg aaatcgcacg gtacacgaga
     4801 aagggggcac catgaaaatc catcaactgt cgctgttcct cgagaacaag cccggacaat
     4861 tgaccgcccc gtgccgcgag cttgcagatg cagggatcga tatcagaacg ctctctcttg
     4921 ccgacacaca gcagttcggt atcctgcggc tgatagtatc agactggaaa cgaggatatg
     4981 agctgctgag agacaagggg tacgtggtca aggacacaga agtggtggcg attgaggtac
     5041 cggatcgccc gggcgggctg ctggaagtcc tcaaagtgtt cgaaaagagc gcagtcaaca
     5101 tcgagtacat gtacgctttt acgtttggca gaggcgacaa ggcggttctg attttccgat
     5161 tcgacgaccc cgacgccgcg atcgcgctcc tcaattcagc cggcatcaac gtcgtcggta
     5221 gcgtcgaagt gtacaaccgc tgacgggaag gagacgccgg caagcctgcg aaggcagctc
     5281 cggtaaaaaa ccgacgagat ggcagaattc ctttttgaaa accggatctg ggatccggcg
     5341 gaaacacttc caaggaacga gcttcaagcg cttcaggtac gtcgactccg tgaatgcgtt
     5401 gcccgcctgg cacacgtccc gttctacagg aaggcctttg ctgaaggcaa cgtgagcgct
     5461 gacaaaatcc ggtctctcga cgatttgaaa cgcctgcctc tgacaacgaa ggcagacctc
     5521 cgcgaacact acccgctcgg gttccttgca actccccgca gcgaaatcgt gcgcatccat
     5581 ggttccacag gaacaaccgg aaaaccaacg ttcgtcgcgt acacccggga tgaccttgcg
     5641 ctctgggcga acctctgcgc gcgatttctg gtcgccggcg ggctcaggcc ggagcacatg
     5701 gttcacattg ccttcggata cgggctgttc accggtggct tcgggctgca ttacggggcg
     5761 gagctggttg gagccgcgat agtccccgcg gcagcgggaa acaccgaacg ccagatcgtt
     5821 ctgatcaacg acttgcaggc ggatgtcctc gtgtgcacgc caagttacgc gctcaacatc
     5881 gcagaaatga tgcgcgcaca aggatacaca ccggacaaga tccccctcaa gttcgcgcat
     5941 ttcggtggtg agccctggac tgaggacatg cggctggaga ttgaaaggca aatgggtttg
     6001 cttgccttca acaactacgg cctcagcgag gtcatcggcc ctggcgtcag cggggagtgc
     6061 gttgtgcgca acggaatgca tatccaggag gaccatttcc tcgtggaatg cattgatccg
     6121 gataccctgg agccagtccc ctacggcgaa ccgggcgagc ttgttcttac cgcgctcacg
     6181 aaacaggcga tgccgattct gcggtaccga acacgcgaca tcgcccgact ggacccgacg
     6241 ccgtgcccct gcgggagaac tggcatccgg atgggaagag tcatcgggcg cagtgacgac
     6301 atgctgatca tccggggagc aaacgtcttc ccatcgcaga tcgaagaggc gcttcttcgc
     6361 gtcgaaggaa cggcaccgca ctacctgatc gaagtcgacc gcccgggaac actggacgag
     6421 gtaaccgtcc gggtcgagct gcgtccggag cgtttttcgg acaagatgag tcagatgcat
     6481 gtgttgcggg aagacattga acgccagatc catatcgtcg ccggaatccg ggcgaacgtg
     6541 gagcttgtgg cacctcagac tctcgaacgg ttcatcggga aagcgaagcg tgtggtagat
     6601 catcgcaagg aaaaaggcct catctgacct gaaggtgtac atgggaatcg ccaagacaat
     6661 cgcgacaaac ctggaacaat catcctggat ccgccggatg tttgaggaag gcatccgttt
     6721 gagggcggaa cgcggcccgg aacacgtgta cgatttcacg ctcggcaacc ccgacattga
     6781 acctccgccg gaggtaattg cggttctgga gcgcatcgtc gcgcgaaacc gcccacgcag
     6841 ccacgcgtat atgcccaacg ccggatttcc cgaggtacgc gccgttatcg ctagaaaatt
     6901 ggcgcggcga accggccttg cgtataccgc agaccacata ttgatgactg cgggcgctgc
     6961 gggcgctatc aacgtagtgc ttaagtcgct tctcgatcct ggtgatgaag taatcgtcct
     7021 ggcgccgtac ttcgccgagt accggttcta catcgagaac catggcgggg tgatggtggt
     7081 tgttcctaca gacgatgcgt tcatgcctga tacgcgaaga atccgcgcgg cgattacatc
     7141 gcggacgaag gcgatcctca taaacacccc gaacaacccg accggagcag tgtacggccc
     7201 cggggtcctc agaagcttga acgagttgat cggggaggtg aatcacccga tcacaatcgt
     7261 cagcgatgag ccctatgtcg ccctcacatt cgacggtgtc gaaccgccgg agaccgcatc
     7321 tctcatctcc cgctccgtcg taacgtattc ctggtcgaaa gcgctggcaa tccccggcga
     7381 acgcatcggt tacatcgcca tttcgccgct gcttcccgaa gcggccgatc ttttcgacgc
     7441 gtgtacgttc gccaaccgcg ttctggggtt cgtgaatgcg tcggcgatct ggcagctcgt
     7501 ggtggccgag gcaggcgatg tgcgagttga cgcaacgccg tatcaggaga aacgtgattt
     7561 tctgtgggac gccctgaccc gaatgggtta cgaaatcgtt aagcccggag gggctttcta
     7621 tctgttcccg aaatcgccgc ttcccgatga cgtggcgttc attcgattac tgcagaaaga
     7681 gggaatcctc gcagtaccag gatcagggtt tggcacaccc ggctatttcc ggctctcgct
     7741 caccgttcca aaggagatga tggaacgttc gctccccggt ttcgagcgaa ctttgcacgc
     7801 cgcagaaggc aacggcaagt cttagcgcag gttgtcggat ttcctcaaaa tcggtttttt
     7861 caaggctcgg gcgtgggcgc tcctgcccga atggcacaga agcctgattc ccggttgagg
     7921 acgtggagaa ccaatgtccg gccacctcaa agcgttgtcc gtcgtcacgt catgccttct
     7981 cctgaatacc tccctccccc ttgccgttcg cgcgggcgtt ccagatcccc ggaccacaat
     8041 tctcgtcgga gtctggggtt atccgccggc gcctgacgat cctgccgatc cgtttccacg
     8101 cttcacagga tccggcactc ctgactggtt cgtcgccaac taccgcgcgc tcaaggtcaa
     8161 ttattacagc tacgacagca taaaagccgc gggaattgac atgaccatga ccccgctcgg
     8221 ggtcaatctc cagaatgaag ggcattgttt tgcgacggta cgcgctgcaa acgctgcgcg
     8281 gcttttctgc ctgcccctcc atcccgacgt gtacggccag cgggaaagcg atcacctcga
     8341 cgttgcgcgg gtacggcgcg cgctgagcaa tccggtctgg gccacgtctg aagattcctc
     8401 cgtgatcggg tggtacatct gggacgagcc acaaaacgat cgcgaagtgg acaaggcgtt
     8461 gcggcttgat tcactgctca gagcacagct cacagaaatc ggaccattgg cgggaaagga
     8521 ctggctcacg ttcctcggtt ggttcgtatg gaacgacagc ctcaggatcg cgagaatact
     8581 gagggggatt ggcacaaccg gcgccggcgg accagccgta atcggccatg aggcattccg
     8641 cttcatgaat ccagacaccg cagagaaccc tcacatctac ccgatggact ggttgaacgc
     8701 cagtctcgcc cgattctatc gcacggtccg ggacaatgcg ccgggcacac cctggtggtc
     8761 ggtaacccat gtcaccgatg agtttggcat gggtgggccg tgtgtgtggt atccctatgg
     8821 cgtgattgac gaggcgagcc ttcggatgga cgtctttacg cggttgggct ggggagcgaa
     8881 aggaatagtg tatctcacgt attcccatta cattgatgcg atcgttttgc ccgattacga
     8941 gaattgcaga cccacaggtc gaacgcgggt gtacgatggg cggtactggg gcgcggattg
     9001 ggcggaccgt catcacacgc gccgatacgc tcggtttccc ggtgttttca cctctgaatg
     9061 gtcatctgag acgatgtcgt accggccgac gggattcgcc gcgatttacg gccatatacg
     9121 tgccgtcaac gcagacgtcg cgcgaatccg cccctatctc ggtcccgcgg agatattcca
     9181 ccccgtcatg tcagatccgc ccgccgtcgg cggtccatac cccaccagat cattctttgt
     9241 tgacggagta tcggcttggg agccggattc agtaatgcgt cccttcaaat cgggatgggg
     9301 agaacgtgac aaggcggcag ttctcgtcac gccgatgaaa aacggccgcg accattacct
     9361 gttgattgtc aatcagaaca tccgttaccg ggagggaacc gttttcgaat caatcagaac
     9421 cggggaggcc ggtgattccg cgttcgttac cgttagaatc aatgcggagc tcatcggcgg
     9481 cggaggggga tacgtggcaa agaacctcgt cactggctca ataaccaaaa aactcatccc
     9541 ttctggccat ttcgtaactc tatttgacac tctagatacc ggcgaattcc gaatgtatca
     9601 aatcatgaag gatacgtcct cacacgtggg acgcttgagg tagaagaggt cgggcccgct
     9661 cgcctcttat cccgcgagcc cggcctccgc gacgaacaac actctcgttt tgcccccgtt
     9721 cacttcatca tcggcaattc tttaccggtt cttgccggct catgtcgttc cgctttccgt
     9781 gcggtcaggt tgctcacact caccgcc
//
LOCUS       DAOTNG010000089         9803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6402_length_9803_cov_5.664239, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000089 DAOTNG010000000
VERSION     DAOTNG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6402_length_9803_cov_5.664239"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="P5179_05815"
     CDS             complement(<1..360)
                     /locus_tag="P5179_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94769.1"
                     /translation="MNPDYRYHGRRMTFITAPGATQAERHVARVASEAARRRGVTGHC
                     HESGDVPSTDLAVFLGVGETHPHVVEELSRRDRFAPSSPLTRGAQSSRIVSCVNQSAD
                     AVAIWGADIEGLWVAVGR"
     gene            complement(414..1187)
                     /locus_tag="P5179_05820"
     CDS             complement(414..1187)
                     /locus_tag="P5179_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS94770.1"
                     /translation="MFVENVVYRADNERGVLDIMLPEGTGPFPAVLCIHGGAWSGGDK
                     SMMHPFGEEFLKLGIASLFPNYRLTGTHPHPAQEQDIFAALEWAAANAERFRLDATRF
                     GITGASAGGHLTALVGLKATKKRDIGYTVRCMAPVCPPTDFFAFSRDNPSLRSVLEAL
                     VGGELEKNEAVIRDVSPIFHVHEKAPPCLVTHGAIDSVVPPAQATSFVDALKSVGVEA
                     EVILVPGVDHAAFNPGTDPPEPLGGIKAFRAFYRKHLLS"
     gene            1314..2084
                     /locus_tag="P5179_05825"
     CDS             1314..2084
                     /locus_tag="P5179_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94771.1"
                     /translation="MLKKFFWNLYDHLGTCIVLNLLWFLVSLPWFLAIWSAGRLLVSS
                     FGAVGFVTALAAGFAAIWLNPATLTAMFCATRWVRYQRVERADAWRFFAAQLVPGIAL
                     SAAAALPLVILGANAAFYLRASWVPRFVGLLLAGVMLWAQIAFLLVAFWFGLNRAAGD
                     DGARFREVVRDSFLLTFRYPARSAGLFVSTFVMGAAMALTQVGLVLIAMSLAAVFAAT
                     GRRELLRAGKPERSEENPDAPEEPRTFRDLFAPWEANR"
     gene            complement(2152..5079)
                     /locus_tag="P5179_05830"
     CDS             complement(2152..5079)
                     /locus_tag="P5179_05830"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS94772.1"
                     /translation="MERKDFLNPAGIYRPAPFWSWNDDLRNEELEAQALDMKKRGWGG
                     YFMHSRIGLVTPYLSDEWMDRTRHTVELSAREGLCAYLYDEDKWPSGYAGGLVPRSNP
                     AVRNTALQCHPVPPPEDTNAVLAVFARESGRWVRIDPASVRSGTELAYISQWVEPMGN
                     PWFNGTTYVDLMNPEAFEEFARSTLDPYAALLGTHCGTTIPGCFTDEPAYIFWHARGI
                     HPSNTVPWSPGFRERFTGRWGYDILDRIISVFKREGDFARVRYHFWRTATEMFLDNFS
                     EPYGRWCREHNLELTGHYMCEDTLVDQIQWVGAAMPHYEHMGWPGMDHLGRNIDNIMT
                     AKQVTSVAHQIGKGRALSELYGCSGQNLTFEGRKWIADWHFVHGINLLNPHLALYSMR
                     GERKRDYPPTISFQQPWWRFNNLIADYEARLSYALTRGKRISQVLVIHPIESAWALYE
                     PGDDRRARQLSEWFDQVSRWLLEEHYDYDYADESLLAKYGRLEHDRLFVGDAEYELVV
                     IPPCFTLRRTTVDFLQKWMAAQGAVISMKPVARRIDAETDAWDFVRDTIVVENDRTAF
                     TSVLGELLEPNVRILSPEGYPVAPVWVHQRADGEKDLFFFANTDRTRGYACEILLQGD
                     GAVEEWDPRTGDVHEVAVEVLDDEVVVSRFVEPCGSLLLVMDRSKDAREIHEDEEEFV
                     EEGDEIDLDGSWLSEPRDPNAITLDTVRWRLGDGELQGPDPLWKVLDPVRRHSGRFSL
                     QFSVSVEIVPEPDVFLVLETPEAFSVSVNGTELPQTDCGFWVDTSFRKRSLNGLLRMG
                     ENTIVLSGTADPKIELESLYVIGDFGVRTEDNRAFVITSRKSVVLAQNLVEEGFPFFA
                     GTISLTQSFEMNLSASGQAKLVFDDPQFVVADVWVNGNNAGQVVWSPYEVEIGRFLVD
                     GTNTIRVDLVNSLRNLLGPHHHAFGELLGVGPDSFSDAENWTDVYQFVPFGFGGARVI
                     VES"
     gene            complement(5176..5976)
                     /gene="murI"
                     /locus_tag="P5179_05835"
     CDS             complement(5176..5976)
                     /gene="murI"
                     /locus_tag="P5179_05835"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRS94773.1"
                     /translation="MDRRPIGVFDSGVGGLTVVNEIQRQLPKERVVYFGDTARFPYGS
                     KSVETVTEFARQDSQFLLGFDVKLIVVACNTATACALDHLREHLSVPVVGVIEPGAVA
                     AVRASRRKFVGVIGTQGTIASGAYQRALTRIDPSISVFAQACPLFVSLAEEGWTDRAA
                     SLLIAHEYLDPLREAMIDVLVLGCTHYPLLMPVIRRVMGPETILTDSAAEVTREVGHL
                     LQENELESDGSAVSGHRFFVSDAPLKFREVGERFLGRALPTVELATAG"
     gene            complement(5986..7194)
                     /locus_tag="P5179_05840"
     CDS             complement(5986..7194)
                     /locus_tag="P5179_05840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRS94774.1"
                     /translation="MIPRRTRIAMGLAAAALFLCANRVPAATGTVVVVANGGNRVISS
                     SVEHGVTFVSVPDLARALELQTEWDARTRTLVVLSGETEAEFIAGNTLVVVGDSTFHL
                     RHGTVLRAGELLAPADALPGLFGLLSPVALLWDDRERVFRTSADFGSGARDGVTDDTH
                     PNGRNRWEIDRVVIDPGHGGRDPGTLSVEGVPEKEITLAIALRLAQLLRDELGVDVVM
                     TRTDDQSVSLRERGLAGVRAGGKLFISIHCNAIDKAHVSGVQTYFLSDAETDEARAVA
                     RAENAALMYEEHADTLSEGDDREMEEILAGLVSDRFLKESQELAALVQRELVRGLRTR
                     DMGVHQAGFYVMKGTLGAMPSILIETGYLTNQDEAARLRDARYQARIADAVLRAVREF
                     KMRYETELGR"
     gene            complement(7194..8582)
                     /gene="miaB"
                     /locus_tag="P5179_05845"
     CDS             complement(7194..8582)
                     /gene="miaB"
                     /locus_tag="P5179_05845"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRS94775.1"
                     /translation="MRGRQLDVLGCDAGASESHVNTTEHMRVYVETYGCQMNVADSEL
                     ITGILSRAGFQMVSGPESADVILLNTCAVRENAEERVFGRLGELTQYKNSRPHVLMGV
                     VGCMAEHLREEILGRAKAVGLVVGPDSYRRLPHLIESALAGPVVDITLDKSEQYLGLE
                     PDRGGGVTAWIPIMRGCNRFCTFCVVPYTRGREKNLPPEEIVRQVEHAVNLGHSEVCL
                     LGQTVNSYRHGDTDFADLVRMVGAVDGIRRLRFMSPHPVGFTASLVDAIAEVPAVCPH
                     VHLPLQSGSDAVLTRMRRDYSAQEFRAVLSDLRHRIPGISVSTDIVVGFCGETEDDHR
                     RTVEFVEESRFDFAFTFVYSPRAGTFAARFLKDDVPAEVKRRRISEVIALQERISADI
                     HAKLVGSVREVLFEGAAKKGDAQSFGRTADFKGVVVEGSFAPNSFRSVRITGANAHTL
                     TGEVLPDTGARP"
     gene            complement(8579..>9803)
                     /locus_tag="P5179_05850"
     CDS             complement(8579..>9803)
                     /locus_tag="P5179_05850"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRS94776.1"
                     /translation="ARSDADCRKLIRRAKAMNPAGKVVVTGCLAERTPDALAGMPEVA
                     AVIGNRAKKNLLSIVTAELQKASGPLIASQDQSGPGFLPLSPRVGSLSATRTRATVQV
                     QEGCDEHCTYCIVPRVRGRSRSRPIEDIVAETETLVADGSVEIALTGVNTGAWGLDLG
                     AEGGIAAVVDAILRVHTPSGQRVERLRLNSLEPQAITPDLVRKCAMEPRVCRHFHVPL
                     QSGSDAILRKMNRRYTAAEYAQKIELIAEHIPDVAIGADVMVGFPGETDEQFRETFEL
                     IRRLPLTYLHVFPFSPREGTPALRLGPAISSERKQERSRALRELGNRKRLQFHLRHVG
                     NVVTVLLEGKPVTATTVLRGLTDNYIRVAVQAPSNRINTLVPVYVEDASREGVRGVLV
                     DECSTSFSRTRGSQK"
BASE COUNT         1978 a         3114 c         2711 g         2000 t
ORIGIN      
        1 gcgccccacg gcaacccaaa ggccttcgat gtccgccccc cagatggcaa ctgcatctgc
       61 gctctggttc acgcacgaga caattctgct cgattgagcg ccccgcgtca gcggcgatga
      121 aggcgcgaag cgatcacgac ggctgagttc ttctaccaca tgcggatggg tttctcccac
      181 acccaggaag acggccagat cagtcgaagg aacgtctccg gattcgtggc aatgaccggt
      241 caccccacga cgacgcgcgg cttccgaagc cacccgtgcc acatgtcgtt ccgcctgtgt
      301 cgcacctggc gcagtgatga aggtcatgcg tcgtccgtga taccggtagt cgggattcat
      361 ttggctgcag actcctcgcg gtcaggcgct gcacgtgaaa cgcgatcgtg gcttcaggag
      421 agaagatgct tccggtaaaa cgcacggaac gctttgatgc cgccaagtgg ttccggcgga
      481 tcggtgccgg gattgaacgc cgcatggtcc acaccgggaa ccagaatcac ctctgcttcc
      541 actcccacgg atttgagcgc atcaacgaat gaggtagcct gtgccggcgg caccacgcta
      601 tcaatcgcgc cgtgcgttac gaggcaggga ggggcctttt cgtgcacatg gaaaatcggc
      661 gacacgtccc ggattacagc ttcgttcttt tccagctcgc cgcccacaag ggcctccagg
      721 accgagcgta aggacggatt gtccctcgaa aaggcgaaga agtcagtggg tgggcacacc
      781 ggtgccatac atcgaaccgt gtacccgatg tccctcttct tcgtggcttt caggcccaca
      841 agtgcggtga gatgcccgcc tgcggaggcg ccggtgatcc cgaaccgagt tgcgtcgagt
      901 cggaacctct ccgcgttggc cgccgcccat tccagcgcag cgaaaatgtc ctgttcctgc
      961 gccgggtgtg ggtgggttcc ggtaagccga taattgggaa agagtgatgc gattcccagt
     1021 ttcagaaact cctccccgaa agggtgcatc atgcttttgt ccccgcctga ccacgcgccg
     1081 ccatggatac acagaacagc cgggaaaggg cctgtccctt cgggaagcat gatgtcgagc
     1141 acgccacgtt cattgtcggc acgataaacc acgttttcca cgaacatttg ccaaatcctt
     1201 tgagaaaatg accacctgat ctcgcaggaa cagatgcgcc tatctttcaa agtaattggt
     1261 tcatttgcac cggagaaacg cctcctcgtt ggcagtggcc agacggagaa cgcatgctga
     1321 agaagttctt ctggaacctt tacgaccatc tgggtacgtg tattgtgctg aatctgctgt
     1381 ggttcctggt aagtcttccc tggtttcttg cgatctggag cgcgggcaga ttactggttt
     1441 cgagctttgg cgcagtcggg tttgtgacgg cactcgcagc agggtttgcc gcgatctggc
     1501 tcaatccggc aacattgacc gcaatgttct gcgccacgag gtgggttcgt taccagcgcg
     1561 tcgagcgggc tgacgcgtgg aggtttttcg ctgctcaatt ggttccaggg atcgcgttat
     1621 cagccgccgc agctctgccc ctggttattc tcggcgccaa tgcggcgttc tatcttcgcg
     1681 cgtcgtgggt gccacggttt gtcggcctgc ttctggcggg agtaatgctg tgggcgcaga
     1741 tcgcgttcct gctggtggcg ttctggtttg gtttgaaccg cgcagccggc gacgacggag
     1801 ccaggttccg cgaggtagtt cgcgattcgt tcctgctgac attccggtac cccgctcgtt
     1861 ccgccggtct gttcgtttca accttcgtta tgggtgccgc aatggcgttg actcaggtcg
     1921 gcctggtgct catcgccatg tctctggcgg ccgtattcgc ggctaccgga cgacgagagc
     1981 tgttgcgagc gggaaaaccg gagcgaagcg aagagaaccc ggacgctccc gaggaaccac
     2041 gaacgttccg cgacctcttc gctccctggg aggcgaacag gtagcctgaa aacggcgtgt
     2101 gatggcggca gccggagcca ccgccaatcc cctcaccggc aacccggaag ctcacgactc
     2161 cacgatcacc cgtgctccgc caaacccaaa cgggacgaac tggtacacat cggtccagtt
     2221 ctctgcatcg ctgaagctgt caggccccac gccgagaagc tccccgaacg cgtgatgatg
     2281 cggcccgagg aggttgcgta acgaattgac cagatccacc ctgatggtgt tcgttccgtc
     2341 aacaagaaag cgcccgattt ccacttcgta cgggctccac accacctgtc cggcgttgtt
     2401 gccgtttacc cacacgtcgg ccacaacgaa ctgtggatca tcgaacacga gcttcgcctg
     2461 cccggatgcg gaaaggttca tttcgaagga ctgggtcagg cttatcgttc cggcgaaaaa
     2521 cggaaatcct tcctcaacga ggttctgcgc aagaaccaca gacttcctgg acgtgatcac
     2581 aaaggctcgg ttgtcttctg tcctcacacc gaagtcaccg atcacataaa gtgactccag
     2641 ctcgatcttc gggtcggccg tgccgctcag aactatcgtg ttctcgccca ttctcagaag
     2701 cccgttcaat gagcgtttcc gaaaactggt atccacccag aacccgcagt cggtttgcgg
     2761 caattcggta ccgttcacac tgaccgaaaa cgcttcgggc gtttctagaa cgagaaacac
     2821 gtccggctcc ggaacaatct ccacggagac cgaaaactgc agtgagaacc ttccggaatg
     2881 gcgtcgcacc ggatcaagaa ctttccagag cgggtccggc ccttgaagct ccccgtcgcc
     2941 aagtctccag cggaccgtat cgagggtgat cgcgttcgga tcacggggct cgctcaacca
     3001 gctgccatcg aggtcaattt catccccttc ttcgacgaat tcttcttcgt cttcgtggat
     3061 ttcccgcgca tccttcgatc ggtccatcac cagcagcagg gaaccgcagg gttcaacaaa
     3121 cctgctcaca acgacctcgt catccagcac ctcgacggca acctcatgaa cgtccccggt
     3181 acgcgggtcc cattcctcca ctgccccgtc tccctgcagc agaatctcgc acgcgtaccc
     3241 acgggtgcgg tctgtgttgg cgaagaaaaa gaggtctttc tccccgtccg ctcgctggtg
     3301 cacccagacg ggcgccacag ggtaaccttc cggggaaaga atgcgaacat tcggctccag
     3361 aagctcgccg agaacgctcg tgaatgccgt tcggtcattc tccacgacga tggtatctct
     3421 aacgaaatcc cacgcgtccg tttccgcatc aatcctgcgc gccacaggtt tcattgagat
     3481 caccgcgccc tgtgccgcca tccacttctg caggaagtca acggtcgtcc gtcgcaacgt
     3541 gaaacatggc gggatcacca ccagttcgta ctcagcgtcc ccaacaaaca gcctgtcgtg
     3601 ctccagtctg ccgtattttg ccaggaggga ttcgtcggca tagtcatagt cgtagtgctc
     3661 ttccagcagc caacggctga cctggtcaaa ccattcgctc aattgccttg cgcgtcggtc
     3721 atccccgggt tcgtacaggg cccacgcact ttcgatcggg tgaatgacga ggacctgcga
     3781 aatccgtttc ccgcgagtaa gcgcgtacga caatcgcgct tcgtaatccg cgatgaggtt
     3841 attgaagcgc caccacggtt gctggaacga gattgtggga gggtaatcac gtttccgttc
     3901 gccgcgcatc gagtacaacg caaggtgagg gttcagaagg ttgataccgt gcacgaagtg
     3961 ccagtccgcg atccatttgc gcccttcgaa cgtcaaattc tgcccggagc acccgtacaa
     4021 ctcagagagt gcgcggccct tcccgatctg atgagcgacc gatgtaacct gcttcgcagt
     4081 catgatgttg tcgatgttgc gcccgagatg atccattccg ggccagccca tgtgttcgta
     4141 atgcggcatg gctgcaccga cccattgaat ctggtcgacg agggtgtcct cgcacatgta
     4201 gtgcccggtc aattcaaggt tgtgctcgcg gcaccagcgt ccgtacggct cactgaaatt
     4261 gtcgagaaac atttccgtcg ccgtgcgcca gaagtggtag cgcacgcggg caaaatcccc
     4321 ctcgcgcttg aaaaccgaga taattcgatc gagtatgtcg taaccccatc ttccggtgaa
     4381 ccgttctcta aagccgggtg accacggtac ggtgttcgaa gggtggattc cccgtgcatg
     4441 ccagaagatg taggcaggct cgtccgtgaa gcagccggga atggtcgttc cgcagtgtgt
     4501 cccgagaagc gccgcatacg ggtcgagagt tgatctcgca aactcctcga acgcctcggg
     4561 attcatcaaa tcgacgtagg tcgtcccgtt gaaccaggga ttccccatcg gctcgaccca
     4621 ctggctgatg tacgccagtt ccgttccgga acgaactgac gcggggtcta tccgaaccca
     4681 tctgcctgac tccctggcaa aaaccgcgag aacggcattg gtgtcttctg gagggggcac
     4741 cggatggcac tggagcgcgg tgtttctcac tgcaggattc gatcgcggaa ccagtccgcc
     4801 tgcgtacccg gatggccatt tatcttcgtc gtagagatac gcgcaaagac cctcccgggc
     4861 ggaaagttcc accgtgtgcc gggtccggtc catccattcg tcgctcaggt agggagttac
     4921 cagaccgata cggctgtgca tgaaataacc gccccacccg cgttttttca tgtcaagcgc
     4981 ctgtgcttcc agctcctcat tccggagatc gtcgttccac gaccagaatg gcgcggggcg
     5041 gtagattccc gcaggattca gaaaatcttt ccgttccata gtattccctt tcgcaggtat
     5101 tcgcgaactc cgatccggcc ctcccggcgt ggttggttcc aactacggtc aagccccaca
     5161 cgtactgccg tccgttcagc ccgccgtcgc cagctccacg gtgggcaatg cgcgcccgag
     5221 aaagcgttcg ccaacctcgc ggaatttcag gggagcatcg gacacgaaaa aacgatggcc
     5281 ggaaacggcg cttccgtcgc tttccagctc gttctcctga agcaagtgcc ccacctcccg
     5341 ggtaacctca gccgcggagt cggtgaggat ggtttccggt cccatgacac ggcggataac
     5401 aggcatcaac agagggtaat gcgtgcagcc caggacgagc acgtcaatca tcgcctctcg
     5461 caaaggatcg aggtactcat gcgcgatcag aaggctcgct gcgcggtctg tccacccttc
     5521 ttcggcaagc gatacgaaca acgggcaggc ctgcgcgaac acgctgatcg aagggtcgat
     5581 ccgcgtcaat gcgcgctgat acgcccctga ggctattgta ccctgggttc cgattacgcc
     5641 tacgaacttc ctcctgctgg ctcggaccgc agccaccgct ccaggctcga ttactcccac
     5701 aaccggaacc gacagatgct ccctcaaatg atccagcgca cacgcggtgg cggtattgca
     5761 ggcaactaca atgagcttga catcaaaccc cagaaggaat tgtgaatcct gccgggcgaa
     5821 ctcggtaacc gtttccacgg atttcgaacc gtaagggaac cgtgcggtgt ccccgaagta
     5881 caccacgcgc tctttcggca attgtctctg gatctcgttc accacggtga ggccgccaac
     5941 ccccgaatcg aaaacaccta tgggtcgcct gtccatcatt cgctcctacc tgccgagttc
     6001 ggtttcatac cgcatcttga attcgcgcac ggcgcggagt acggcatccg cgattctcgc
     6061 ctggtatcgc gcatcccgaa gtctcgcggc ctcatcctga ttggtgaggt agccggtctc
     6121 gatcaggatg ctcggcatcg cgcccagcgt tcccttcatg acatagaagc ccgcctgatg
     6181 cacacccata tcccgggtac ggagacctct caccagttcc ctctgaacca gcgcggccag
     6241 ttcctggctc tccttcagaa aacgatcaga gaccaatccg gccagaatct cttccatttc
     6301 ccgatcgtcg ccttcactca gagtgtccgc gtgctcctca tacatgagcg cggcgttctc
     6361 cgcacgggcg actgcacggg cctcatccgt ctcagcatcc gaaagaaagt acgtctgaac
     6421 tccgcttaca tgggccttgt cgatcgcgtt acagtggatg cttatgaaca gcttcccgcc
     6481 cgcgcgaacg cctgcgaggc ctcgttcccg aaggctgact gactggtcat ccgttcgggt
     6541 cattacgaca tctacaccga gttcgtctcg cagaagctgc gcaagtcgaa gggcaatagc
     6601 gagcgtgatt tccttctccg gaaccccctc cacgctcaat gttcccgggt ctcgtccgcc
     6661 gtgccccgga tcgatgacga ctctgtcgat ctcccatcgg ttgcgcccat tcggatgtgt
     6721 gtcatccgtg actccgtcac gcgcgccgga gccgaaatcc gcggacgtgc ggaagacacg
     6781 ctcccggtcg tcccacagaa gcgctactgg cgacaggaga ccgaacagcc ccggcagggc
     6841 gtctgccggt gcgagaagtt cccccgcccg gagaaccgtg ccgtggcgaa ggtgaaaggt
     6901 ggagtcgccc accaccacaa gcgtattgcc cgcgatgaat tccgcttccg tttcgcccga
     6961 aaggacaaca agcgtccgag ttctggcatc ccattcggtt tgcagctcca gggctcgcgc
     7021 caggtccggc acgcttacga acgtaacccc gtgctccacg ctggaactga taacccgatt
     7081 gccgccgttg gcaaccacca cgaccgtccc cgttgccgca gggacccgat tcgcacacag
     7141 gaagagagcg gccgccgcca gtcccatcgc gatccgcgtc cggcgaggta tcatcacggc
     7201 cgtgctcctg tgtcgggaag gacctcgccg gtgagcgtgt gggcgttggc gcccgtgatt
     7261 cgaacggacc ggaacgaatt cggagcaaac gagccttcca ccaccacccc tttgaaatcg
     7321 gccgttcgac cgaagctctg tgcatccccc ttcttggcgg caccttcgaa cagcacttcc
     7381 ctgaccgacc cgacaagttt cgcatggata tcggcggaaa tgcgctcctg caacgcgatc
     7441 acttccgaaa tcctgcgccg tttcacttcc gcaggaacat catccttaag aaaccgcgcg
     7501 gcgaaggttc ccgcccgtgg cgagtacacg aatgtgaacg cgaagtcgaa gcgactttcc
     7561 tcaacaaact cgacagttct ccggtggtcg tcttcggttt ccccgcagaa accaaccacg
     7621 atgtccgttg aaaccgaaat tccggggatt cggtgccgca ggtctgacag caccgcacga
     7681 aactcctgcg ccgaataatc cctgcgcatc cgcgtcagaa ccgcatcaga acccgattga
     7741 agcggcaggt gcacgtgagg acatacggca ggaacctccg caatcgcgtc cacaagcgat
     7801 gcagtgaacc ccaccggatg ggggctcata aagcgcagac gcctgattcc gtccaccgcg
     7861 cccaccatgc gcacgaggtc ggcaaagtca gtatctccgt gacgatatga attcacagtc
     7921 tggcctaaca ggcatacttc gctgtgtccg agattcaccg cgtgttccac ctgccgtacg
     7981 atttcctccg gcgggagatt tttctccctg ccgcgcgtat acggaaccac gcagaacgtg
     8041 cagaagcggt tgcaaccacg catgatcgga atccacgcag tcacacctcc accgcgatcg
     8101 ggctccagcc cgaggtactg ctcgctctta tccagcgtga tatcgactac cggaccggcg
     8161 agcgctgatt cgatcaaatg aggaagcctc cggtacgagt cgggcccgac aacgagcccc
     8221 accgctttcg cccgcccgag gatttcttcc cgcaggtgtt cggccatgca acccaccact
     8281 cccatgagaa cgtgcggccg cgagttcttg tactgcgtaa gctctcccag cctgccaaat
     8341 acgcgctcct cggcgttctc tcgaactgca cacgtattca gcagaatcac gtcggcactt
     8401 tccggaccgg aaaccatctg gaaacccgct cgggagagta tcccggtgat cagttccgaa
     8461 tccgccacgt tcatctggca cccgtacgtc tccacataga ctcgcatgtg ttctgtggtg
     8521 ttcacatgtg actcactcgc gccggcgtca cagccgagta cgtcgagttg acggcccctc
     8581 atttctgact ccctcgagtc cgtgaaaatg acgtggagca ttcgtctacc agaacgcccc
     8641 gcaccccctc ccgggaagcg tcttcgacat acacgggcac caacgtgttg atgcgatttg
     8701 aaggagcctg aacggcgaca cggatgtaat tgtcggtcaa tccacgaagc accgtggtgg
     8761 cggtcactgg tttcccttca agaagaaccg tcacgacatt gcccacgtgt cgcaggtgaa
     8821 actgaaggcg ctttctgttt cccagttccc tcaacgcgcg gctgcgttcc tgcttccgct
     8881 ctgaggagat tgcagggcca agtcggagag ctggcgttcc ctcgcgtggg gaaaagggga
     8941 agacatggag atacgtgagc ggcaagcgcc gaatgagttc aaaggtttcg cggaattgtt
     9001 catccgtttc gcccgggaat cccaccatca cgtccgcgcc aattgccacg tcaggaatat
     9061 gttccgcaat gagctctatc ttctgcgcgt attccgcggc agtgtagcgc cggttcatct
     9121 ttctgagaat cgcatcgctc ccgctctgca gaggcacgtg aaaatggcgg catacacgcg
     9181 gttccatagc gcacttgcgc accagatccg gtgtgatcgc ctgcggttcg agggagttga
     9241 ggcgaaggcg ttcaaccctc tgtcccgacg gagtgtgaac gcgaaggatg gcgtccacga
     9301 cggcagcgat gcccccttcg gctccgaggt cgagccccca ggcacccgtg ttgacacccg
     9361 tcaacgcaat ctcaacggaa ccatctgcaa ccagagtttc cgtttccgcg acgatgtcct
     9421 caatcggtct tgaacgactt cgaccgcgaa cgcgcgggac aatgcagtac gtgcagtgtt
     9481 cgtcgcaccc ttcctgcacc tgaaccgttg cgcgtgttct cgtcgctgag agagatccaa
     9541 cccggggcga aagcgggagg aaacccggcc cgctctgatc ctgcgaagcg atcaaagggc
     9601 cggaggcttt ctgaagttca gcggtgacga tggaaagcag gtttttcttc gcgcggtttc
     9661 cgattacggc tgcaacctcc ggcataccgg ccagcgcgtc cggggtcctt tccgcaaggc
     9721 aaccggtaac cacaactttc cccgcggggt tcattgcttt cgcgcgtcgg atgagtttgc
     9781 ggcaatcggc atcgctccgc gcc
//
LOCUS       DAOTNG010000090         9799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6407_length_9799_cov_5.325431, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000090 DAOTNG010000000
VERSION     DAOTNG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6407_length_9799_cov_5.325431"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="P5179_05855"
     CDS             complement(<1..529)
                     /locus_tag="P5179_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS94777.1"
                     /translation="MLSGVVPALMTPFTAGGEKYDRNRLRGLCEHLISSRVGGLFVTG
                     TTGEFIALSPEERRQVIEDAVEFAGVRTKVIVHACSYNTMESVALAEFACRVGADGVG
                     SLPPYFHPMDEEAQYAFFSRMCEAVKGRPFYLYNLPAYAANEISLPLIRRLKAAFPHL
                     KGIKDSSGDLERMKNE"
     gene            complement(728..1417)
                     /locus_tag="P5179_05860"
     CDS             complement(728..1417)
                     /locus_tag="P5179_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS94778.1"
                     /translation="MKFGKRLQRERERRGLTQRELGLKVGLTSGTYISQIESGEKIPP
                     LETAIKLANALGLADTVPFVFHAVRERSAVEYGFLSSRIKPPPPGKNEPCPRPLPVRP
                     WASVVEGNLPTGTEGSPADRRCIVLEGSGDADSFAVEISTDWMADTLCRGDVVVVSPG
                     SEVASGDMALVKVTGKRGTNVEEIKIARAYFRGRWTDLVPGDLSKYSVETYPSHDITV
                     LGKVVAKLSRL"
     gene            1765..1953
                     /locus_tag="P5179_05865"
     CDS             1765..1953
                     /locus_tag="P5179_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94779.1"
                     /translation="MVLQRLFSVHPPENGPFKPDSVVASEPKRIFVNGKPVDGWIITV
                     KSDEDEVHLMLENIEDVE"
     gene            2248..3294
                     /locus_tag="P5179_05870"
     CDS             2248..3294
                     /locus_tag="P5179_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94780.1"
                     /translation="MLALVSGGLDSILAARVIQEQRVEVLPVHFLHPWGCGSWEAVRL
                     ITRQLGVEPRIVEAGQDFIAAVRNPRFGRGRNFNPCVDCRVFMFRSLFSLMAETGSEA
                     IITGEVLGQRPMSQLRKKMILVDREAGLEGRVLRPLSAQRLPPTVPEIANIVDRRALF
                     GFTGRSRAPQRELARHLGVSEFQTPAGGCHLTNPSFVPKLRDFFAHYPDSALTEARAL
                     NLGRHLRISPDLKLIVARNESEGRELEALHDFSEPAEMPRSVYIPDGFDGPTVLACGR
                     VGTEEEELIGGVILRYSRRQNPREPGGFPLRVVSSGNTRRVHARNALPPDIVRRFLIS
                     DLTSSMRPCRDEVE"
     gene            complement(3262..3852)
                     /locus_tag="P5179_05875"
     CDS             complement(3262..3852)
                     /locus_tag="P5179_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS94781.1"
                     /translation="MSASSTQPIPGTKSLDYLLDSRESRMEMRTATEGVMSGFAPGYT
                     FVFYAFEGFIRLTPGSITPAEFVMVTKADSIRVVGRTRESEKAMVEGILKNEMMETAR
                     YPEIVYRGSNIALEKTGPATYRATIDGTMSQHGVTRRCPIVVDVVVTDTNVRMRGSFT
                     ILQSDYGMRPKRLAGGLVRVKDRVFLSFDLVATRAH"
     gene            complement(4052..5122)
                     /gene="galT"
                     /locus_tag="P5179_05880"
     CDS             complement(4052..5122)
                     /gene="galT"
                     /locus_tag="P5179_05880"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRS94782.1"
                     /translation="MSIVSRLRPRPPYYDPKQWELRWHPLRGEWVVIAAHRDTRPWKG
                     GSVSPPPKEPEYDPTCYLCPGNIRTTGGVNPRYRTTFAFDNDFPPAGTSSPLKTSSHG
                     VYRAARAYGRCRVMCFHPRHDLTLATMDVQEIRRVLSLWQREYRLLGEDPRVKHVLIF
                     ENKGEITGTSNRHPHCQAYGTTFIFSTIQREIQLAKRFLRRTGTPLYRAVLEQELEDG
                     CRIIARRGSIIAFVPYFARYPYEVHVAPLESRPSIADLTGSEADDLACVLKAVLVRYD
                     NLFTMPFPYVQVLHQAPTDGTHHPEFHFHIELYPPLRNPVTLKYLAGPEIGGGNMLSD
                     VAPEEKARELQVQPEIHYSRRV"
     gene            complement(5157..5807)
                     /gene="folE"
                     /locus_tag="P5179_05885"
     CDS             complement(5157..5807)
                     /gene="folE"
                     /locus_tag="P5179_05885"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRS94783.1"
                     /translation="MSDEYQRMHPVTDMERDGDYALPPDYERIEKLAYHVSEIMKLLH
                     LDLRDGNLLDTPERVAKLYLEVFSSLDESREPRITVFDNEERYSSMVTVKDIPFYSMC
                     AHHFIPFFGVAHVAYIPTAKIIGLSKIPRIVKFYSRMPQLQERLTEQIANYLDEKLEP
                     RGTYVVMEGRHLCMEMRGIESNGAKTVTSALRGCFAKPEIREEFLDLLKIARWEGH"
     gene            complement(5981..6211)
                     /locus_tag="P5179_05890"
     CDS             complement(5981..6211)
                     /locus_tag="P5179_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94784.1"
                     /translation="MRSLFRRPRRKCNYQLTKWIRGEENVQFAVEQLEQHGLPYEIRR
                     SRNGMLSVWTYFDELTGDENESNRLERLLKAS"
     gene            complement(6273..6581)
                     /locus_tag="P5179_05895"
     CDS             complement(6273..6581)
                     /locus_tag="P5179_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRS94785.1"
                     /translation="MRQVSAIEVFANTFPEVTHAYRTLKANYRGKGPLDDKTRELIQT
                     AVMVVMNSEEGTRDHARLAAEAGATPDEIIQTVLMVLGPAGITRTSAGLQWVKEALAG
                     "
     gene            complement(6646..6852)
                     /gene="iscX"
                     /locus_tag="P5179_05900"
     CDS             complement(6646..6852)
                     /gene="iscX"
                     /locus_tag="P5179_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRS94786.1"
                     /translation="MKLTWDDAEEIAAALAEHHPDVDVLGVHFTDLHRWVVELPGFAD
                     DPKGSSEGKLEAIQMAWLEEVQSS"
     gene            complement(6871..7188)
                     /locus_tag="P5179_05905"
     CDS             complement(6871..7188)
                     /locus_tag="P5179_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRS94787.1"
                     /translation="MPRITFLPADVTVEVSEGTSILDAALDNGISLTHNCGGVGACST
                     CHVIIRGGEDSLSEITQAEDDRLSMAEGLTLHSRLACQAFVGTEDVVVEIPVITSRLS
                     HDA"
     gene            complement(7219..9066)
                     /gene="hscA"
                     /locus_tag="P5179_05910"
     CDS             complement(7219..9066)
                     /gene="hscA"
                     /locus_tag="P5179_05910"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRS94788.1"
                     /translation="MSRILGIDLGTTNSLIAQVVDGVPHVIRDPQTGERLLPSVVAFT
                     DDGKVLVGQAARDAERNGSGIVISSVKRLMGIGMEHVNEDDRKQFPLTPADSGPVRFT
                     VGNQSMTPPQISAHLLRELRRRAEQALGEPVSDVVITVPAYFNDSQRQATRDAGRLAG
                     LQVLRLVNEPTAASLAYGLDRRDHGTVAVFDFGGGTFDVSILRLQGGIFEVLATNGNT
                     RLGGDDFDRAVAGWLLKKAADESGLPEIMTDHRVFTQARREAENAKIRLTTESVTVAT
                     LVLPGMDTPYEALLTREEFETIIRATAEQTIVHCREALKDAQISPKDVDSVVLVGGST
                     RVPLVRTLVREFFGIEPLADIDPDEVVALGAAVQADILAGGTKDMLLLDVVPLSLGIE
                     TMGGVMTRLIHRNTKIPCTVVEEFTTPADNVTSIDIHVLQGEREFARDNRSLARFKLP
                     VEPLPAGMARVLVMFLIDANGILSVTAIDEKTKNEHTVEVKPSYGLTDEQVEEMLIDS
                     FDNAERDVTERLLAEAVVAAESILLHTNKALKEGADLLQPEERDGIVRAESALRKALE
                     STDRQTILDATAALDAATKPLADRLLDRAVNRLLKSRTVDEVLASDEAV"
     gene            complement(9063..9665)
                     /locus_tag="P5179_05915"
     CDS             complement(9063..9665)
                     /locus_tag="P5179_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRS94789.1"
                     /translation="MTPSHDNARAPEIHHDPDSNNYFDVLGLPTRLALNEKDLSDHYY
                     ALSRLYHPDFHQNALPAERLRALRRTAAVNDAFTTLRDPVSRGRWWLEFNGRKLSLAP
                     GLPRELGSLVFDVHDALERFRNAEADITEIERFREVTRDELGRRNKQLAALFGELDEA
                     GVSRADGLLFTRLENLLAEISYLRALTRDIERTLENTGER"
BASE COUNT         2006 a         2871 c         2790 g         2132 t
ORIGIN      
        1 gttcattttt catccgttcc agatcaccgg aagaatcctt gatacccttc aggtggggga
       61 aggcggcctt cagacgccga atcaacggca agctgatttc gtttgcggcg tacgcgggaa
      121 gattgtaaag gtaaaacggc ctgcccttga ctgcttcaca catccgactg aagaatgcgt
      181 actgcgcttc ctcatccatc ggatggaaat atggtggcag actcccgacg ccgtccgcac
      241 caacccggca cgcgaactcc gcgagcgcaa ccgattccat ggtgttgtaa ctgcacgcgt
      301 ggacgatgac tttcgtgcgg acacccgcga attcgacggc gtcctcaatc acctggcgcc
      361 gctcttccgg gctcaaagca atgaactcgc cagttgtccc ggttacaaaa aggccgccca
      421 cgcgggacga gataaggtgc tcacaaagtc cgcgaaggcg atttctgtcg tatttctcac
      481 cgcctgcggt gaaaggagtc ataagagcag ggacaacacc ggaaagcatt cgaaaggcgc
      541 ctttcgcaag attgcgggtt cgtgttcctg ggagatttgt atgaacatat agtgccttgt
      601 ccccgggctg caaggccacc ttttgcggga gacgaccgca gcatcaccag gtggcgccgc
      661 ggctctcaat caccagaaaa ccagagcacg cgaacgcggc acggggaaac cacaccagaa
      721 ggagacatca aagacgactg agtttggcaa ccacttttcc gagaaccgtt atgtcgtgtg
      781 acggataggt ttcgaccgag tacttgctca aatctcccgg aacaaggtcc gtccatcgcc
      841 ctctgaagta tgcccgcgcg attttgatct cctcgacgtt ggttccgcgt tttccggtta
      901 ccttgacaag tgccatgtcc ccgctggcaa cttcgcttcc cgggctcacc acgaccacat
      961 caccgcggca taacgtgtcg gccatccaat ctgtgctgat ttcgacggcg aaggaatcgg
     1021 catcgcctga cccttccagt acgatgcaac gccgatcagc tggggatcct tcggtgcccg
     1081 tcgggaggtt accttcaacc accgatgccc acggacgaac cggcaacggc cgcgggcagg
     1141 gttcattctt gccaggcgga ggcggcttga ttctcgacga gagaaaaccg tattcaaccg
     1201 ccgaccgctc ccgcaccgcg tggaagacga agggaactgt gtctgccaga ccgagcgcgt
     1261 tcgccagttt gatggccgtc tccagaggtg ggatcttctc ccctgactca atctgggaga
     1321 tgtaggtccc cgaggtcagg ccaaccttga gcccgagttc acgttgtgtc agcccgcgcc
     1381 gttcacgttc tcgttgcagc cttttcccga atttcatgag attcatcctc gggcagatgc
     1441 cgtgacccgg tgggccgcag gcacggtgcc gtgtgaactc gttgcgttga ccggcggcat
     1501 cctctgcgtt tgatcagcag tgtcggaagt tccttcgcca accacccgac cctccaaaat
     1561 tacggttctt atgccgcatc gaggcaatcc ctgcggcgga ggcgagtgct cgaggcgtac
     1621 cgccccgcgt gtcggctaac gtaatgacct ggatcgcacc aaaccggcac ggcatcgcac
     1681 caggttgccc gaggttgcgt aagtgaaacc acagtcgcta cattgtacca gggttttcaa
     1741 tgagaacgac gcggggagtc tgtgatggtg ctgcagcgcc ttttcagtgt tcaccctccg
     1801 gaaaatgggc cgttcaaacc ggattctgtc gttgcgagcg aacccaaaag aatattcgtc
     1861 aacggcaaac ccgtggacgg ctggatcatt acggtgaagt cggatgagga cgaagtccat
     1921 ttgatgcttg aaaacatcga ggatgtggaa tagaacctga cgttcaaacc ataccggagg
     1981 ctgtatgagg gaaagagaac gtgaactgcg gcgtcaaagg aaccgaagaa tcaaacgcgt
     2041 gaaagcaagg ttgagggaac agaaggcgca ggcactgacc gtgcggaacg aacgcgcgca
     2101 ggaaaagcag aagaaacggg tgcgccgggt atcctccgca gaagaagcgt cggcgtagtt
     2161 cgtgccgtgt tgataacgcg attgccgtcg agcgccgttt ttgcgccgct cgacggttct
     2221 tttttggggt gaccaatggc gtaccgcatg cttgcacttg tttccggtgg actggacagc
     2281 attctcgccg cgcgggtcat tcaggaacag cgcgttgaag tgctgcccgt ccatttcctc
     2341 catccctggg gatgtggaag ctgggaggcg gtccgtctca tcacccgcca actgggcgta
     2401 gagccccgaa ttgtggaagc aggacaggat ttcattgcgg cggtgaggaa cccacgattt
     2461 gggagggggc gcaatttcaa tccctgtgta gactgccgtg tgttcatgtt ccgatccctt
     2521 ttttcactca tggcggaaac cgggagcgaa gcgatcatca caggcgaagt gctcggtcag
     2581 cggccaatgt cgcaactgag gaagaagatg atccttgtcg acagagaggc cggactcgaa
     2641 gggcgtgtcc tgcgaccact ttccgcacag aggcttccac cgacggtgcc ggaaatcgcg
     2701 aatatcgtag acaggcgggc tctttttggt ttcacgggcc gatcacgggc accacagcgg
     2761 gagcttgcgc gacatctggg agtgtctgaa tttcaaacgc cggcgggtgg atgccatctg
     2821 acaaacccca gtttcgtgcc gaaactccgc gatttcttcg cccattatcc tgactccgca
     2881 ctgactgaag cacgtgccct caacctgggc cggcacctgc ggatatcacc tgatttgaag
     2941 ctcattgtcg cgcgcaacga atcggaaggg cgggaactcg aagccttaca tgatttttcc
     3001 gagcccgcgg aaatgccgcg ttccgtctac atccctgacg ggttcgacgg ccctacagtt
     3061 cttgcctgcg ggcgtgtcgg aacggaagaa gaggagttga tcgggggtgt gatactgcgg
     3121 tactccagac gccagaatcc tcgcgaaccc ggcggattcc cgctgcgtgt ggtctcttca
     3181 ggaaacacgc gccgagttca cgcccggaac gcgttgcctc ccgacatcgt gcgtcgtttc
     3241 ctgatttccg acctcaccag ttcaatgcgc ccttgtcgcg acgaggtcga atgagaggaa
     3301 gaccctgtcc ttcacccgca ccaatcctcc tgcgagtctt ttgggacgca tgccgtaatc
     3361 agattgcaga atcgtgaagg agccacgcat ccttacgttt gtgtccgtca caacaacgtc
     3421 gacaacgatg gggcaccgtc gcgtgacacc gtgttgtgac atcgttccgt caatggtcgc
     3481 ccggtacgta gctggccccg ttttctcgag tgcaatgttg ctaccccggt acacgatttc
     3541 agggtaacgg gcggtttcca tcatctcgtt tttcagtatc ccctcaacca tggctttctc
     3601 cgactcgcga gttctgccga ccacgcgtat cgaatccgcc ttcgtgacca tgacgaactc
     3661 agccggcgtg atgcttcccg gtgtaagccg gatgaatccc tcaaatgcgt agaacacgaa
     3721 ggtatacccg ggtgcgaacc cggacatgac tccttcagtg gccgtgcgca tctccatgcg
     3781 actttcccgt gaatcgagga ggtagtccag cgatttcgtg ccagggatgg gttgcgttga
     3841 ggacgctgac acggatgttc cggcagcaaa gaccaccaga acggcaggca atatgaacat
     3901 ccggcagtgc atacctgatt actccgaaag gggcttcgcc gcacactcac ctgaagcgac
     3961 cagcgtcgga acggtctcac aggttctgcg aacaggcaca ggtgtagggt tcgcttctgc
     4021 ggcgcgatcg ccatgtagcg tggccgtgca atcaaacccg ccgggaataa tgaatctccg
     4081 gttgcacctg aagctccctc gccttctcct cgggcgcaac gtcactgagc atgttgcccc
     4141 ctccaatttc cgggccggcg aggtacttca gcgtgacagg attgcgtaat ggcgggtaga
     4201 gctctatgtg gaagtggaac tcggggtggt gtgttccgtc agtcggagcc tgatggagta
     4261 cctggacata aggaaacggc atcgtgaaaa ggttatcgta ccgaaccaga acggctttga
     4321 ggacacaggc gaggtcgtct gcttcggaac ccgtcagatc tgcgatcgaa ggtctcgact
     4381 cgagtggtgc cacgtgaact tcgtacgggt atctggcgaa atagggaacg aacgcgatta
     4441 tgcttcccct ccgtgcgata attcggcatc cgtcttcaag ttcctgttcc aataccgcgc
     4501 ggtacaacgg tgttccggtt ctcctgagaa agcgctttgc caactggatt tcgcgctgaa
     4561 tcgtggaaaa gatgaacgtc gttccgtatg cctggcaatg cggatgacga ttgcttgttc
     4621 ccgtgatctc gcccttgttc tcgaaaatga gaacgtgttt gacgcgagga tcctccccga
     4681 gcagacggta ttcccgttgc cacagcgaga ggacgcgtcg gatttcctga acatccatgg
     4741 tagcaagcgt gagatcgtgt ctcgggtgga aacacatgac ccgacacctg ccgtacgccc
     4801 gtgcagcgcg gtatacgccg tgggagctgg ttttcagcgg ggaggaagtc cctgcgggtg
     4861 ggaaatcatt gtcgaaggcg aacgtcgtcc tgtaccgggg gttcacgcct cctgttgtgc
     4921 gaatgttgcc ggggcacagg tagcaggtcg gatcatattc cggttccttt ggcgggggac
     4981 ttacagaccc gcccttccag ggccgagtat cgcgatgggc tgcgattacg acccattctc
     5041 cccgcaacgg atgccaacgg agttcccact gtttcggatc gtaatacggg gggcgaggtc
     5101 gcaggcggga aacgatggac atcagaagcc ctttcccagg tccttcggcg tgcgtttcaa
     5161 tggccttccc agcgcgcgat tttgagcaag tcgaggaatt cctcgcgtat ttccggtttg
     5221 gcgaaacacc cgcgcagcgc tgaggtcacc gttttcgccc cgttggattc aattccccgc
     5281 atctccatgc agagatgtct cccctccatt acgacgtacg ttcctctcgg ttccagtttc
     5341 tcgtcaaggt agttcgcgat ctgttcggtc agtcgctcct gcaattgggg cattctgctg
     5401 taaaacttga cgattctcgg gatcttgctc agtccgatta tcttcgcggt tggaatgtag
     5461 gcgacgtgcg caactccgaa aaacggaatg aagtggtgag cgcacatgga atagaagggg
     5521 atgtccttga ccgttaccat ggagctgtat cgttcttcgt tgtcgaacac cgtgatgcga
     5581 ggctcacggc tctcgtccag cgaactgaag acttcgaggt agagcttcgc cacccgttcc
     5641 ggagtatcaa gcaagttacc atcacgcaaa tcgagatgga ggagcttcat aatctcggag
     5701 acatggtaag cgagtttttc gattcgttcg taatcagggg gcagcgcgta gtcgccatcc
     5761 cgttccatgt cggtaacggg atgcatccgc tggtattcat cggacatggt ggtgggcctt
     5821 ttgcatgcga acagaggaag gaggcgtatc ccgcctccaa acgcttgtcg ttacataagt
     5881 aacgtacggt tacgggggga aaaaggaaag accccacggc cggctccctc tagggacccg
     5941 gatgaaccga agggtcttca cggttggcgc tgcgttggtc tcaactcgcc ttcagaagac
     6001 gctccagtcg gttggattcg ttttcatcgc cagtgagttc gtcgaaatac gtccacacgg
     6061 agagcatccc attgcgggat ctccgtattt cgtacggcaa cccatgctgc tccagttgct
     6121 caacggcaaa ctggacattc tcttccccgc gaatccattt ggtcaactgg tagttgcatt
     6181 tgcgccgcgg ccttcggaaa agggacctca tatgtcaccc accttgtatt ttggcgcaca
     6241 tggcgcccgc gtctggttct gttttcaggt catcagcctg caagtgcttc cttcacccac
     6301 tgaagccccg cactggtacg cgttatgccg gccgggccca gcaccatcaa gacggtctgg
     6361 atgatttcat ccggagtcgc ccctgcttcc gcggcaagtc tggcgtggtc acgagtgccc
     6421 tcttctgagt tcatgaccac cattactgcc gtctggatga gctcccgtgt cttgtcgtcc
     6481 agagggccct taccgcggta atttgccttg agagtgcggt acgcgtgcgt cacttctgga
     6541 aacgtgttcg cgaaaacctc gatggccgac acttgtctca ttgtaaaaac ccctcagggt
     6601 agcaaatggt tcccgagaaa tgttccgcga ctccgaacgc cgtcttcaac tggactgaac
     6661 ttcctccagc cacgccatct ggatggcctc gagtttgccc tcgctcgacc ctttcgggtc
     6721 gtcggcgaaa ccggggagtt caacaaccca cctgtgcaga tcggtgaaat ggactccgag
     6781 aacgtccaca tccgggtggt gttcggcgag ggcagcggcg atctcctcgg cgtcatccca
     6841 cgtgagtttc atcgcattct ccgggagaac ctacgcgtcg tggctcaacc gagaggtaat
     6901 cacaggaatt tcaaccacaa catcctcggt tcccacaaaa gcctggcacg cgagtctcga
     6961 gtgaagagtc aacccctccg ccatgctcag ccgatcgtct tcggcctgcg tgatttcaga
     7021 cagcgaatct tcgccgcccc tgatgataac gtggcacgtt gaacacgcgc ccacgccacc
     7081 gcagttgtgc gtgagcgata tgccattgtc caacgcagcg tcgaggattg aagttccctc
     7141 gctgacctca accgtcacgt cggccggtag aaacgttatg cggggcattg cggtcctgtc
     7201 tgctccaatc ggtgtcccct acacagcctc atcagaggcc agaacttcgt ccacggttcg
     7261 actcttcaga agacgattca cggcgcgatc cagcaagcga tccgcaagcg gcttcgttgc
     7321 cgcatcgaga gcagcggttg cgtcaagaat cgtctgccga tcggtggatt caagagcctt
     7381 tctcaacgcg ctctccgcgc gcacaatccc gtcgcgttcc tcgggttgga gcaggtcggc
     7441 accttccttc aacgccttat tcgtgtgaag caggatgctt tcggccgcca cgaccgcctc
     7501 ggcgaggagg cgctccgtga catcccgctc cgcgttgtcg aacgagtcta tcagcatctc
     7561 ctccacctgc tcgtctgtca acccgtagga cggcttcacc tccactgtgt gctcattttt
     7621 ggtcttttcg tcgatggctg tgacactcag gatgccgttg gcgtcgataa gaaacatgac
     7681 gagcactcgc gccatgccgg caggaagagg ctcgaccggc agtttgaacc gcgcgaggct
     7741 ccggttatcg cgcgcgaatt cccgctcgcc ctgcaacacg tggatgtcaa tgctcgtgac
     7801 gttgtccgcg ggcgtggtga actcctcgac aaccgtacag ggaatcttcg tattgcgatg
     7861 aatgagccgc gtcatcacgc cgcccatcgt ctcgattccc agactgagcg gaacgacatc
     7921 gagcaagagc atatccttgg ttccgccagc cagtatatcg gcctgaactg cggctccgag
     7981 agcaaccact tcgtccggat cgatatccgc gagcggttcg atcccgaaga attcacgcac
     8041 cagtgtccga acaagcggga ccctcgtgga accacccacc aggacaaccg agtcaacatc
     8101 tttggggctg atttgcgcgt ccttgagcgc ttctcggcaa tgtacaatgg tctgttccgc
     8161 cgtagcgcga atgatggttt cgaactcctc cctcgtaagg agcgcctcat agggagtatc
     8221 cattccgggg agaacgagcg ttgcaacggt aactgattcc gtcgtgaggc gaattttcgc
     8281 gttttccgct tcacgccgtg cctgagtgaa aaccctgtga tctgtcatga tctcgggcag
     8341 ccccgactcg tcggctgcct tcttcagaag ccatcctgcc accgcacggt caaagtcatc
     8401 tcccccgagt ctcgtatttc cgttcgttgc caggacctca aaaatcccgc cctgcagacg
     8461 gagaatcgag acgtcaaacg tgcctccgcc gaagtcgaaa actgctaccg taccgtggtc
     8521 acgtctatcc agaccgtacg cgagactcgc cgcggtcggt tcgttgacaa gacgcagcac
     8581 ctgcagcccg gcgagccgcc ccgcatcgcg tgtggcctgg cgctggctgt cgttgaagta
     8641 cgcggggacc gtgatcacca cgtcgcttac cggctcgcca agagcctgtt ccgcgcgccg
     8701 tctcagctct cggagcaggt gcgcggagat ttggggcggc gtcatggact ggttgccgac
     8761 ggtgaatctg acgggccccg aatctgccgg agtcagcggg aactgcttgc gatcatcttc
     8821 gttcacgtgt tccatcccga tgcccatgag ccttttcacc gaagaaatga caatgccgga
     8881 gccatttctt tctgcatcgc gtgcggcctg gccgacaagc accttaccgt catcggtgaa
     8941 cgcaaccacc gacggcaaaa gacgttcccc cgtctgcggg tcacggatta cgtgaggaac
     9001 accgtcaaca acctgcgcga tcagactgtt tgtcgtaccg aggtctattc cgagaattcg
     9061 gctcatcgtt cacccgtgtt ttcgagggta cgctctatat cccgcgtgag cgcccgcagg
     9121 taggatatct cggcgagaag attttcgagg cgcgtgaaca gaaggccgtc ggcgcgggaa
     9181 actccggcct catcaagctc gccgaaaagt gcggcgagct gcttgttccg acgcccgagt
     9241 tcatcccgcg ttacttccct gaagcgctcg atttccgtga tgtctgcttc ggcgttgcgg
     9301 aatctttcca gcgcatcatg gacgtcgaac acaagggatc ccagctcgcg aggtaatccc
     9361 ggggcgaggg acagttttcg accattgaac tcaagccacc aacggccgcg gctgacagga
     9421 tctcggagag tggtgaacgc gtcattcact gccgcagtgc gacgcagcgc ccgcagtctc
     9481 tcggcgggca acgcattctg atggaaatcc ggatggtaga ggcggctcag cgcatagtaa
     9541 tggtcagaca aatccttttc gttcagcgcg agcctggtgg gaagtcccag gacgtcgaaa
     9601 tagttgttgc tgtccggatc atggtggatc tccggcgccc gggcattgtc gtgagaaggt
     9661 gtcatatccg gaacgaggtg ccacatccgc aggtggactt gacattcgga ttcttgatct
     9721 cgaaccgttc ctccatcagg ttcttgacgt agttgaaatc cgaacctcga acgtagatcg
     9781 cgcttttcgg gtctatgag
//
LOCUS       DAOTNG010000091         9710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6467_length_9710_cov_4.826515, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000091 DAOTNG010000000
VERSION     DAOTNG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_9710_cov_4.826515"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..2144
                     /locus_tag="P5179_05920"
     CDS             189..2144
                     /locus_tag="P5179_05920"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRS94790.1"
                     /translation="MPIPALPTTPFGVIKETWKPWLEALEAWQPSREVVRPHLYQPAG
                     DRNIVRERLSAFPGTIEQYRKAAESALEAEILSPDKGLRAVGTLIGRATACANWWLLS
                     LDERAARRAWEISLAIMEWPRWVADEHLPLTIDLKSAAAVQALSNVLDRMSDWLQPDQ
                     KATIANCLQDRGVLPFVAIHDSHGEWWTYSLHNWRSVIDGAFGIAAIAVRDTLPTDMV
                     KRALKHSVIGVLTVLDQGDEDGGWYEGVSYWSYGIGEAVQFMDVLQRITEGRVDLFTH
                     PYIGKTGDFCLYETWPDGRVHNWGDCGDRVNAAALMARLAAARQRSDWQAYVRKFPAA
                     PSIESLFWEDPSLQASDLASLPRAKHFRGTQVAVIRSGWADDDVIVGLKAGETTANHS
                     HLDMGSFVINCGRHCLVADAGHWPYAHSLGFFDLDFPDGARWDFPGLATECHSTILMD
                     GQGQQYGEGRDAVVVACADGDDYAYATIDATSAYPNAARFVRYVLLIRPDTIVVVDDL
                     ASPSRCRFGWRVVVPEVPVRGNSIKEWSLTTSDGSASLSIRCLLPGEENGLLAEVSQL
                     AASYPATSTQANPTCQTLTFSNLVRARQATFAVAMRVTRGPLMPPAIEVERIYRGLKI
                     HALNAGKETWAIMWGEPGVRKLTCTPG"
     gene            2182..3270
                     /locus_tag="P5179_05925"
     CDS             2182..3270
                     /locus_tag="P5179_05925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS94791.1"
                     /translation="MNRIVHQLDPGNENPRNSEGSFVTLTDGTILFAYSRFTSSGSDF
                     GSADIAAIYSHDGGETWSSDPQIIVRNTGGINVMSVSLLRLQDGRIALFYLVKSSEND
                     CRPCMRTSDDEGGTWSAPINVIPTPGYYVVNNDRVVQLKSGRIVIPAAFHRRKTVTDT
                     SWKAVDSRGIMLAFLSDDAGATWRESKSWWALPVPGNSGLQEPGCVELTNGQLYGYCR
                     TDTGYQWSTVSRDGGDTWSPPEPSQFISPNSPLSMKRLPPSEPGKNGHLLAIWNDKSP
                     RFASQVRPSSPSARTPLVSAISEDEGRTWAHHRLLESDPESGYCYTAIYMLPNACLLA
                     YCAGSKATGSILNTLRIRKVDYLWFYAT"
     gene            complement(3353..4798)
                     /locus_tag="P5179_05930"
     CDS             complement(3353..4798)
                     /locus_tag="P5179_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRS94792.1"
                     /translation="MRPWNDTAWKNSMRVRLAMANVFEEAVGSEGGIARARLESLYDR
                     LRAIQREIALKREAGTLPFLDLPKQDVSAILACVEQNRPKFETFVVLGIGGSALGNIA
                     CHTALRHPYHNFLPADRRGGMRIEVADNIDPDRIAGLFDTLDLPRTLFNVITKSGSTA
                     ETASTFLAVKKLLQDHPRIGPGWREHVIVTTDPVAGDLRKFASREGLVSFDIPRGVGG
                     RFSVLTPVGLLSAAMSGIDIEELLAGAADAAEYHASVPAEKCPAFAGAALHYLMYNHP
                     RAPKRLAVMMPYSHALKDVADWFRQLWAESLGKEKALDGTVVNVGPTPIKALGVTDQH
                     SQVQLYIEGPNDKLFTFVETETFQNDLVLPSDNDLSDLGAFNYFAGKTLGQLLHAEQK
                     ATTLALTRRHRPNLTISMREVRPYTVGQLFMLLEIQTVVAGALFGINPFDQPGVEAGK
                     VATYALMDRPGYENEKNAIEQEISTGAEWVL"
     assembly_gap    4945..5044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5045..5741)
                     /locus_tag="P5179_05935"
     CDS             complement(<5045..5741)
                     /locus_tag="P5179_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRS94793.1"
                     /translation="MACDEVLFQLVPNAPSAAILRFYQWKPYAVSVGRSQSPQKEIEL
                     NALRRDGYDCVRRITGGRAVFHVEELTYSVVCAREHPLATGGIHATYERTSEALARGL
                     RLAGIRAEVARVTQHLSPGSPGRSAPPCFGSVARSEIVVDGRKLVGSAQYRSSTAVLQ
                     HGSILTGTAHRRILEYLRVEEDVRQRYLRNLEVGTTCLWELGWRGTTDDLIRALADGF
                     SGHFGVVWESSQGL"
     gene            complement(5801..6622)
                     /gene="rnc"
                     /locus_tag="P5179_05940"
     CDS             complement(5801..6622)
                     /gene="rnc"
                     /locus_tag="P5179_05940"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRS94794.1"
                     /translation="MVWLGRLLSRKGSAAQRTGSAGGIRDLEDAIKYTFRDKSLLVAA
                     LRHRSYVHANGDSPDDLVSQANERLEFLGDAVLGLLATEFLYREFPDKPEGELTKRKS
                     VLVSKSALARCALEIHLDQHVLLSSVEEGMGGRQRRSILGDAFEALVGAIYLDGGLEP
                     SRSFLETTLFVHLREYETESIFTNYKSLLLEHVQAGGGRPPEYMLRSQSGPDHQKTFT
                     VEVAIGGKVLGTGTGTTKKYAQQEAARLAYEFLTRRASNRGDQNTDEPFPATGVQ"
     gene            complement(6670..7920)
                     /gene="fabF"
                     /locus_tag="P5179_05945"
     CDS             complement(6670..7920)
                     /gene="fabF"
                     /locus_tag="P5179_05945"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRS94795.1"
                     /translation="MGNKTRRVVVTGMGAVTPVGNSLEEYWQALLHGKSGAGSITKFP
                     TEGFSTTIAAEVKGFDPTQRINAKEARRMDLFIQYAVYAASEAIEDADLRVDGMDPTR
                     VGVIVGSGIGGISTLEAQYKVLKERGPGRVSPFMIPMLISDMAAGMISIIFGLKGPNY
                     CTVSACASAAHALGDAVSVLRNGHADVVLTGGAEASLTELAMAGFCAARALSTRNDDP
                     EHASRPFDKDRDGFVCGEGAGILVLETLDHAIARGARIYAEVLGFGYSADAYHITAPP
                     ENGEGMARCMENALLDAEMPKERVGYINAHGTSTEVGDAAETAGIKNVFGEHAYRLAV
                     SSTKSMTGHLLGAAGAVESIATILALHHGVLPPTINLSTPDPACDLDYVPNSPREKRV
                     DVAMSNSFGFGGHNASLVFGSWKD"
     gene            complement(7975..8211)
                     /gene="acpP"
                     /locus_tag="P5179_05950"
     CDS             complement(7975..8211)
                     /gene="acpP"
                     /locus_tag="P5179_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRS94796.1"
                     /translation="MAIEDKVRDLVVEQLGVAPEEVKPEARFIEDLGADSLDTVELVM
                     AFEDKFGVEIPDEEAEKLDTVGAAIEYLKSKNVE"
     gene            complement(8337..9083)
                     /gene="fabG"
                     /locus_tag="P5179_05955"
     CDS             complement(8337..9083)
                     /gene="fabG"
                     /locus_tag="P5179_05955"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRS94797.1"
                     /translation="MFDLNGRVVLVTGGSGDIGAALCEKFALAGAKVAMTSRTGSAGL
                     ARVLETLRAHGAETLPISMDVRQRSDVEAGVAKVVETWGRLDVLVNNAGITKDTLLLR
                     MNDQQWNDVLETNLTSVFLCTQAALKPMLKARWGRIISITSIVGEVGNAGQANYASAK
                     AGIIGFTKTVAKECASRNITVNAIAPGFIESRMTDTIPEKVRTTLLASIPLGRPGTPM
                     EVAAAAVFLASEEASYITGHVLNVDGGIAM"
     gene            complement(9100..>9710)
                     /locus_tag="P5179_05960"
     CDS             complement(9100..>9710)
                     /locus_tag="P5179_05960"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRS94798.1"
                     /translation="EFGAALRLVAARAAAMADAASRMPGTMAAVVGLDERTVEKSVEG
                     LRDVVVANYNAPDQVVISGAEEAVGNAGTALSELGAKRIVPLKVSGAFHSPLMAEAAR
                     VFADALEREEISTPGIPVVPNVTARRTTDPEEIRSLLVEQITSPVRWTDTVRSLASSG
                     SASCFEVGPGRVLQGLVRRIAPSITTTTVGTTAEIRALLKTQ"
BASE COUNT         1926 a         2942 c         2709 g         2033 t
ORIGIN      
        1 ccacggaata cggtggcagg gtcattctgt tcgggcgggc gatttttcag gaggaaaacc
       61 cgcgcgcgat gtgcaaagcg cttcgggccg tgctcgaccg ccagatgtca cccgccgagg
      121 cgcattcggc ctaccagcgt acgctcagag cgtgacctcc cccattcccc gatattaagg
      181 acgccaacgt gcccattccc gcactcccca ctactccgtt cggggtaatc aaagaaacct
      241 ggaagccctg gctggaagcg ctcgaggcct ggcaaccgtc tcgagaagtc gtgcgtccgc
      301 acctgtacca accggcgggc gatcgaaaca tcgttcggga aaggctttcc gcattcccgg
      361 ggacgattga gcaatacaga aaagccgcag aatcagcgct cgaagcggaa atcctttcgc
      421 cggacaaagg tctgcgggcg gtcggaaccc ttatcggccg ggccaccgcg tgcgcgaact
      481 ggtggttatt gtcgctggat gagcgagcgg cgcggcgtgc gtgggaaatc tcccttgcga
      541 tcatggaatg gccccgatgg gttgccgacg aacaccttcc cttgacgata gacctgaaga
      601 gcgcggctgc ggtccaggct ttgagcaatg tgcttgacag gatgtctgat tggctccaac
      661 cggatcagaa agccaccatc gcaaactgcc tgcaagatcg cggcgttctg ccctttgttg
      721 caatccatga ttcacacggg gaatggtgga cgtacagcct gcacaactgg cgatccgtca
      781 ttgacggcgc attcggaatc gcggcgattg ctgtacgcga caccctgccg acagacatgg
      841 tgaagcgggc gctaaaacat tccgtgatag gcgtcctcac ggttctcgac cagggcgacg
      901 aggatggcgg gtggtatgaa ggggtatcct actggagcta cggtatcggt gaggccgttc
      961 agttcatgga tgtgcttcag cgtataactg agggaagggt ggatttgttc acacacccct
     1021 atatcggaaa gacgggcgac ttctgcctgt atgaaacctg gccggacggt cgcgtgcaca
     1081 actggggaga ctgcggtgat cgcgtgaacg ccgccgcatt gatggcgagg ttggcagccg
     1141 ccaggcaacg cagcgactgg caggcgtacg tgcgcaagtt cccggcggcg ccgtccatcg
     1201 agtcgctctt ctgggaagac ccttccctgc aggcttcaga tttggcgtcg cttccccgtg
     1261 caaaacactt tcgcggaacg caagtagccg tcattcgttc cgggtgggcg gatgacgatg
     1321 tgatcgtcgg cctcaaagcc ggcgaaacga cggcaaacca ttcccatctc gatatgggat
     1381 cgtttgtcat taactgcggc agacactgtc ttgtcgcgga cgcaggccat tggccttacg
     1441 cgcattccct aggttttttt gatctcgatt tccccgacgg cgcccgctgg gactttccgg
     1501 gattggcaac ggagtgtcac agcacgatcc tcatggatgg gcaagggcag cagtacggcg
     1561 aaggccgtga tgcagttgtt gtcgcgtgcg cggatggcga cgactacgcg tatgccacga
     1621 tagacgcaac gagcgcatat ccgaacgcag cccggtttgt gcgctatgtg ctgttgatcc
     1681 gtcccgacac gatcgttgtc gttgatgacc tcgcgtctcc ttcgagatgc agattcggct
     1741 ggcgggtggt ggtgccggaa gttcccgtgc gtggaaactc catcaaagag tggtccctga
     1801 cgacgtcgga cggctcagct tcgctctcaa ttcggtgtct gttgccggga gaggagaacg
     1861 gcctcctcgc cgaagtgagc caacttgcgg cgtcttaccc ggcgacgagt actcaggcga
     1921 atcccacctg ccagaccctg acgttcagca accttgtacg agcccgtcag gcaaccttcg
     1981 cggtggccat gcgggtaacc cgcggccctt tgatgcctcc ggcaattgag gttgaacgca
     2041 tttaccgagg gctcaaaatc cacgccctta atgccgggaa agaaacgtgg gcaatcatgt
     2101 ggggagaacc cggagtccgg aaactcacct gtacccctgg ctgatgtgct gcaattccgt
     2161 acccagaagg agttcggagc cttgaacaga atagtccatc aacttgaccc cggcaacgaa
     2221 aatccgagaa actccgaagg cagtttcgtt acgttaacgg acggaacgat tctgttcgcc
     2281 tacagccgat tcacctcttc cggcagcgat tttgggagcg ccgacatcgc cgcgatctac
     2341 tcacacgacg gcggggagac ttggtcgagc gacccgcaga tcatagtcag gaacaccggc
     2401 gggatcaacg tgatgagcgt ttccctcctt cgccttcagg acggcagaat cgctcttttc
     2461 tacctcgtca agagctctga aaacgattgc cgtccctgta tgagaacgtc agatgacgaa
     2521 gggggtacgt ggagtgctcc aatcaacgtg atccccacgc caggctatta cgtggtgaac
     2581 aacgaccgcg tcgtgcagct caaatcgggg cggattgtca tacccgcggc gtttcaccgg
     2641 cgaaaaactg ttaccgacac gtcgtggaaa gccgtggatt cgcggggcat catgctggcc
     2701 ttcctatcag atgatgcggg agccacatgg cgggaaagca aatcctggtg ggccctgccc
     2761 gtgcccggca atagcgggtt gcaggaaccc gggtgcgtgg aactcacgaa tgggcagctg
     2821 tacggctact gccggacgga cacggggtac cagtggtcaa ccgtatcacg ggatggcgga
     2881 gacacgtggt ccccacctga accatctcaa ttcatttcgc caaattcgcc cctttccatg
     2941 aaacgactcc ctccttccga gccgggcaag aacgggcatc tgctcgcaat ctggaacgac
     3001 aaatccccga gattcgcatc acaggtgcgg cccagcagcc ccagcgctcg aacccctttg
     3061 gtaagcgcga taagcgagga tgagggacga acgtgggcgc atcatcggct tctcgaaagc
     3121 gacccggaaa gcgggtactg ctacaccgcc atatacatgc tcccaaacgc ctgtctgtta
     3181 gcctattgcg cggggtcgaa ggcgacgggc agtattctca acactctccg cattcggaaa
     3241 gttgattatc tctggttcta tgccacgtag atttcagaca gaagaccacg aaccgtgtgt
     3301 gcgttctgag tgctgttttc cgcggcggag aatgaacctg ttagaccgca catcacagca
     3361 cccactccgc tcctgttgaa atctcctgct ctatggcgtt cttctcgttt tcgtatcccg
     3421 gccgatccat gagcgcgtag gtggcaactt tgcccgcctc cacaccgggt tggtcaaacg
     3481 ggttgattcc aaacagcgcc cccgcaacaa ccgtctgaat ctccagaagc atgaacagct
     3541 ggcctactgt gtacggtcga acctcccgca tcgagatggt caggttcggt cggtgacgcc
     3601 ttgtcaaagc aagggtcgtt gccttctgct ccgcgtgaag caactggccg agcgttttgc
     3661 cggcgaagta attgaaggct cccaagtcag aaaggtcgtt gtccgacggc agaacgagat
     3721 cgttctggaa cgtttcggtt tccacgaacg tgaacagttt gtcgttcgga ccttcgatat
     3781 aaagctgcac ctgcgagtgc tggtccgtca cgccaagcgc ctttatgggg gttggaccca
     3841 cgttcacgac agttccatcg agtgccttct ctttgccaag gctttccgcc cagagctgcc
     3901 gaaaccagtc ggcgacatcc ttcagggcgt gggaatacgg catcatgact gccagtcgtt
     3961 ttggggcacg gggatggttg tacatcaggt agtgcagtgc ggcacctgcg aatgccgggc
     4021 acttctcggc gggtacgctg gcgtggtact cagccgcgtc agccgccccg gcaagcagtt
     4081 cctcgatgtc gattccgctc atcgcggcgg aaagcaatcc caccggtgtg agaacgctga
     4141 atctgcctcc aactccccgt ggtatgtcga aggacacaag accctcgcgc gacgcaaatt
     4201 tccggagatc cccggcgacc gggtcggttg tcacgatcac atgttcccgc catcccgggc
     4261 cgattcgcgg gtgatcctgg agtagcttct tgacggccag gaatgtgctt gccgtctctg
     4321 ccgtgcttcc cgatttcgtg atgacgttga aaagcgttcg gggcagatcc agcgtatcga
     4381 acaagccggc gatacgatcg ggatcgatat tgtctgcaac ctcgattctc atgccgcccc
     4441 ggcgatccgc gggcaggaaa ttgtggtacg ggtgccggag cgccgtgtgg caggcaatat
     4501 tgcccagagc tgatccaccg ataccgagaa ccacgaacgt ctcgaacttc gggcgattct
     4561 gctccacgca cgcgaggata gcggaaacat cctgcttcgg aagatcgagg aacggcagcg
     4621 tacctgcttc tcgtttcaac gcaatctcgc gctgaatcgc cctgagcctg tcgtaaaggc
     4681 tttccagccg ggcacgggct ataccaccct ccgatcccac cgcttcttca aagacattcg
     4741 ccattgcgag gcgtacccgc atcgagtttt tccatgccgt atcgttccag ggtctcatcc
     4801 atgtcctccg tccgtatcac caggattcca gttttctcga ctattccgct catgcactct
     4861 cgtatcaatc aggctcatct cgtgcggcgt aaggccttgg cttgattccc acacgacgcc
     4921 aaagtgcccc gaaaacccgt cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnntaaggc cttggcttga ttcccacacg acgccaaagt gccccgaaaa cccgtcggca
     5101 agcgcgcgga tcaaatcatc agtggtgccg cgccatccca gttcccacag gcaagtcgta
     5161 ccgacttcca gatttcggag ataccgctgt cgaacgtctt cttccacgcg caggtattcg
     5221 aggattcgtc tgtgtgccgt ccctgtcagg atcgaaccat gctgcagcac agccgtggat
     5281 gaacggtact gggcagagcc tacgagtttg cgcccgtcga cgacgatttc ggagcgcgcg
     5341 acgctcccga aacatggcgg agcgcttcgg ccaggggagc cgggcgacag atgctgcgtc
     5401 acgcgggcca cttctgcgcg aattcctgca aggcgtaacc cacgtgcaag cgcttcgctg
     5461 gttcgttcgt aggtcgcgtg gatgcccccg gtggccaggg gatgttcccg cgcacaaacg
     5521 acggaatagg tcagctcttc cacgtgaaaa accgcgcggc caccggtgat tctgcgaaca
     5581 caatcatacc cgtcgcgtcg caacgcgttc agttcgattt ctttctgcgg ggactgggaa
     5641 cgccccaccg aaacggcgta cggcttccac tggtaaaaac ggagaatcgc cgccgaggga
     5701 gcgttcggta caagctggaa aagaacctca tcgcaggcca tattccacgc tgctggcgcg
     5761 gggggcgtta cgatgagacg ccaacattct ttgtccgcgt tcattggaca ccggtggcgg
     5821 ggaacggttc atccgtgttc tggtcgccgc ggtttgaagc ccttcgcgtc agaaattcat
     5881 acgcgagcct cgccgcctct tgttgcgcgt acttcttcgt tgttccggtt ccggtcccga
     5941 ggactttccc cccgatggca acctccaccg tgaaagtctt ctggtggtcc ggcccggatt
     6001 gtgacctcag catgtattcc ggcggccgtc ctcctcccgc ctgaacgtgc tccagaagga
     6061 gacttttgta attggtgaag atagactccg tctcatattc gcgtagatgg acgaacaggg
     6121 tggtttccag gaagctccgg ctcggttcca gtcctccatc gaggtaaatg gcgcctacaa
     6181 gagcttcgaa ggcgtctccc agaatggagc ggcgctgtct gccacccata ccctcctcaa
     6241 cgctgctcaa caacacatgc tgatcgagat ggatttccag cgcgcaacgc gccagtgcgc
     6301 ttttcgatac gaggaccgac tttctctttg tcagttcccc ctcgggtttg tcggggaatt
     6361 cccggtaaag gaactccgtc gcaagcaatc ccagaacggc atcgccgagg aattcaagcc
     6421 tttcgttcgc ctgtgatacg aggtcgtccg gcgaatcgcc attcgcgtga acgtaggaac
     6481 ggtgtcgcag ggctgcgacg agaaggcttt tgtcgcggaa ggtgtacttg atcgcgtctt
     6541 cgaggtcgcg aattcctccg gccgagccgg tgcgttgcgc tgcagagcct tttcgtgaca
     6601 gaagccgacc tagccagacc atcgaaacac atatacctgt ggagaaccac ggaaaacagg
     6661 gaacgcgaat cagtctttcc aggaaccgaa cacgagactc gcgttgtgtc ccccgaaacc
     6721 gaacgagttg ctcatcgcca cgtccacgcg tttttcccgt ggggagttcg ggacgtaatc
     6781 aagatcacac gcggggtccg gcgtggacag gttgattgtc gggggcaaca caccgtggtg
     6841 gagcgccagt atcgtcgcaa tcgattcgac tgcgccggct gccccgagaa ggtgccccgt
     6901 cattgatttt gtactgctga cggcgagacg atacgcgtgc tctccaaaga cgttcttgat
     6961 ccctgccgtt tcggctgcgt cacccacttc cgtggatgtc ccgtgagcgt tgatgtaccc
     7021 gacgcgctcc ttcggcattt ccgcgtccag aagtgcgttt tccatgcacc gtgccatccc
     7081 ctcgccgttc tcgggcggtg ccgtgatgtg gtacgcgtcc gccgaatacc cgaagccgag
     7141 cacctccgcg tagatcctcg cccctctcgc aatcgcgtgg tccagagttt cgagcaccag
     7201 tattcccgcg ccttctccgc acacgaaacc gtcgcgatcc ttgtcgaacg gccggcttgc
     7261 gtgctccggg tcatcgttcc gcgtggaaag tgcccgcgcc gcgcagaatc cggccatcgc
     7321 cagttccgtc agggaagctt ccgctccgcc ggtcagaacc acgtctgcat gtccgttccg
     7381 caggacggac accgcgtcgc ccagtgcatg cgctgccgat gcgcacgcgg acaccgtgca
     7441 ataattcgga cctttgagtc cgaagatgat actgatcatt ccggcggcca tgtccgaaat
     7501 cagcatggga atcatgaacg ggctcacccg ccctggtcca cgttctttca ggactttgta
     7561 ctgcgcctcg agcgtcgaaa taccgccgat acccgagccg acgatcaccc cgacccgcgt
     7621 cggatccatc ccgtcaaccc gcagatcagc gtcctcgatc gcttcagatg ccgcgtacac
     7681 cgcgtactga atgaaaaggt ccattcgccg cgcttctttt gcgttgatgc gctgcgtcgg
     7741 gtcgaaccct ttcacttcag ccgcgatcgt agtcgagaaa ccctcggtag ggaacttcgt
     7801 aatcgagccc gcgccactct tgccgtggag aagagcctgc cagtactcct ctagtgaatt
     7861 acctaccggg gtaactgcgc ccattcccgt gacgactacg cgtcgggttt tgttgcccac
     7921 gaagaactcc ttggacgcga agcgagcgag tgaagtcgcc cgcggccgaa ccgactactc
     7981 cacgttcttg cttttcaggt attcgatcgc ggcgcctacc gtgtcgagtt tttccgcctc
     8041 ttcgtcagga atctcgacgc cgaatttgtc ctcgaatgcc atgacgagtt caacggtatc
     8101 caacgaatct gccccgagat cctctatgaa acgagcctct ggcttcactt cctccggtgc
     8161 aacacccaac tgctcaacta ccagatcgcg aactttgtct tcaatcgcca tattttacct
     8221 cctggtgccc tatgcacgtg gtgtggcggg ttgatgccct gacaagcggt actttaaata
     8281 acactcgttg cgatggcacg ctccgtttga acccaccgac ccgttggccg atccgttcac
     8341 atcgcgatgc caccatcaac gttcaagacg tgtcctgtaa tgtacgatgc ttcctctgac
     8401 gcaaggaaga ccgctgcggc cgctacttcc atcggcgtcc cgggccgccc gagtggaata
     8461 ctggcaagca acgtcgttct cactttttcc ggaatcgtgt ctgtcatccg ggattctatg
     8521 aatccggggg caatggcgtt cacggtaatg ttacgggaag cgcattcttt ggcgacggtc
     8581 ttggtaaaac caatgatacc cgctttcgca ctggcgtaat tcgcctgtcc cgcattccct
     8641 acttcgccca ctatggaggt aatggaaatg atacgtcccc agcgggcctt cagcatgggc
     8701 ttcaacgcag cttgagtgca cagaaagacg ctcgtgaggt tcgtttcaag cacatcgttc
     8761 cattgctggt cattcatccg gagcagcaga gtatccttcg tgatccctgc attattgacc
     8821 agcacatcca gccgccccca tgtctcgacc acctttgcga cgccggcctc cacgtcggaa
     8881 cgctgtcgaa cgtccatgga aatcggcagg gtttccgcac catgcgcgcg cagtgtttcc
     8941 agcacacggg caagtcccgc tgatcccgtt cggctcgtca tcgccacctt tgcgcccgca
     9001 agtgcgaatt tttcgcacag agcagcaccg atatccccgg acccgcccgt aacaagcaca
     9061 actcgaccgt tcaagtcaaa catcgcgaac tgagtcctgt cattgggtct tcaacagtgc
     9121 ccgaatttct gctgtggttc caactgtggt ggtggtgatc gaaggagcaa tgcgtctcac
     9181 aaggccctgc aaaaccctcc ctggccccac ttcgaagcag cttgcggagc cggacgatgc
     9241 cagggagcga accgtgtccg tccaacgtac ggggctggtt atttgttcaa ccagcagact
     9301 ccggatttcc tcagggtcgg ttgtccgtct cgcggtaacg ttgggaacaa caggaattcc
     9361 cggtgtggag atttcctcgc gttcgagggc atcggcgaac actcgtgccg cttcggccat
     9421 cagcggcgag tgaaaggcgc cgctgacttt gagtggaaca atccgcttgg cacccagttc
     9481 cgaaagcgcg gtacccgcgt ttcctactgc ttcttctgct ccggaaatca cgacctggtc
     9541 gggcgcgttg tagttggcaa caactacatc acgtaggccc tcgacacttt tctcaaccgt
     9601 gcgttcgtca agtccaacca ctgcagccat agtcccgggc attcgcgacg ccgcgtccgc
     9661 catcgccgca gctcgcgctg ctacgagacg caatgcggca ccgaactcca
//
LOCUS       DAOTNG010000092         9695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6480_length_9695_cov_5.534025, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000092 DAOTNG010000000
VERSION     DAOTNG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_9695_cov_5.534025"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="P5179_05965"
     CDS             <1..1559
                     /locus_tag="P5179_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94799.1"
                     /translation="GMPAALRVACPTQVGGMVPRRGGPPHSKDRNGDHPMLNYLTGQP
                     LEIGPGPHLFLDDHLIEDRRQLSRRVNPPYRHPGNPIIVADRPWEERPYRPQVIRNPE
                     TGGWRMYYMCFSGTNYWSRKGPSYYACYAESADGIRWEKPERADLPFDPWPATNVLHV
                     DDERDARIQAPWVFFDPHDTDPAHAWKMIYNSAGLRLAYSPDGIHWTPARPEPLFAYH
                     SDTMNHVLWNEQAGKWVLYMRPPMFSSGRHEGPGVEHHRRRTAVSFSDDLLHWSVPRT
                     VLYPDELDPTPHIDSVHVFPYAGHYIAFVTMLDATTGGTNELMLASSRDGLEWERPLP
                     RQVWLSRGREGDFDGGCVSIGSDPIRVGWELWFPMSGFPMPQKVFEQESGIGIVKMMV
                     DRFLSLEAPEPSLGGPEDFGWLLTKEFIWRGKTLTINCRMNNGDSRTYGDLRVEVVKG
                     PNDADPAGRMGQVVPGHGVEDCDLIRSNSPVQTVTWNGNPDLGFLDGQPLYLRFRIRN
                     GGLFAFRMGE"
     gene            1658..1978
                     /locus_tag="P5179_05970"
     CDS             1658..1978
                     /locus_tag="P5179_05970"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRS94800.1"
                     /translation="MEATDARKRAAYFSAALRKRLGANLRQVILFGSRARGDFREGSD
                     YDFAVVVRRKDPTVQSSIVEAGVDFLNRFDEVSACVVYDETEWAESTRLPLGVNIKRE
                     GIAL"
     gene            1975..2187
                     /locus_tag="P5179_05975"
     CDS             1975..2187
                     /locus_tag="P5179_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRS94801.1"
                     /translation="MKDQKNIREILAKAHEKLDSANVLFEHQQYDDAISRAYYAVFHA
                     MTAALFFQDMVFRPTPRQLAHSTANS"
     gene            2184..2369
                     /locus_tag="P5179_05980"
     CDS             2184..2369
                     /locus_tag="P5179_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94802.1"
                     /translation="MKSGVFPVAFTTTIQSLFQNRQVADYGAGKAADRDTALSSLNGA
                     TAVVTAIEKYLADANAL"
     gene            2599..2892
                     /locus_tag="P5179_05985"
     CDS             2599..2892
                     /locus_tag="P5179_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS94803.1"
                     /translation="MALELPLVKIRDICARYGVTELCLFGSAARDDFSPESDVDLLVT
                     FRPDATVTFFTLSRMAAELSAVIGRPVDLVPKTDLKPLLREEVLATTRVLYAA"
     gene            2882..3220
                     /locus_tag="P5179_05990"
     CDS             2882..3220
                     /locus_tag="P5179_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS94804.1"
                     /translation="MRRDRLFLADICSAADDISAFLEGVSRDAFLTNKMLQSAVLQKL
                     IVIGEAAARVSPELRARHPGVPWAEMTAFRNIAVHAYFSINWSIVWATARIDVPQVRN
                     NVQRVMENEE"
     gene            3225..4196
                     /locus_tag="P5179_05995"
     CDS             3225..4196
                     /locus_tag="P5179_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRS94805.1"
                     /translation="MSGPKRVLILQPIHEAGLTALRNAGLDIIHAPDATRETLLSLIP
                     EADGLIVRTTAYRIDAEVMDASRRLKVIGRHGVGVDHIDLAAAEARGITVVNTPLANS
                     QGVAEYTIGLMLSACRFIPRADRAVREGNWPERERLVGDELFRHTLGIIGLGRVGTRV
                     ARIAALGFQMRVLYFDIIRKHETEAALGVEFAPFEEVLRQADFLTLHTPLTEATRGMM
                     NEATLALMKQGAYLVNASRGEVVKIDALARSIATGKIAGAALDVFPDEPFPADHPLLA
                     MPEVIVSPHMASHSRDSMVRMALVAEDVARVLNGQEPLHPVGTGTVS"
     gene            4255..4752
                     /locus_tag="P5179_06000"
     CDS             4255..4752
                     /locus_tag="P5179_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94806.1"
                     /translation="MPQKIMSEIWDYIAQEFRDTISRATPEQLGWRITPKTYSFAETA
                     WRALGAAYLWSVVLSGAKTLEEAITMDPAALDLLQAALNKYAPGHFPDSMPCDQDTLL
                     ERTDEVIALLSAQLVEMSPSMRTKDFRTWWDAVYTGDEIVARLMWRVAHFNGHMHSIL
                     LHARG"
     gene            4757..5158
                     /locus_tag="P5179_06005"
     CDS             4757..5158
                     /locus_tag="P5179_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS94807.1"
                     /translation="MGRTGWLLMVVAMWGTANFLMKVVGSRISSASGVLGIVIGYSAI
                     GLWIALSGGGRIGTGVGYLGALAVGALYIGGNYAYLRLAQREDISAIAPIGSLNVVIP
                     VALGFLLLGEPMTLRKLVGFACALAAVVLLR"
     gene            5174..5905
                     /locus_tag="P5179_06010"
     CDS             5174..5905
                     /locus_tag="P5179_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94808.1"
                     /translation="MPFDLHRQLQELYAGEDGQREVRINGSFVADVVRHGVIYEIQTR
                     KLSNIKSKITSLLETTPIVIVYPVSVHTTIVHCDGADLRECDRRPSPKQGQALDAFGE
                     TVYLAHLLAHPNMALEILLVDTEDIRIKGDPHAATRRRRRRRRHSEWTTVNRRLTATH
                     GRVRLDTPADGLQLLPPAVKFPFCTRDLANATGASLSRAQQILYTLFHMGALRRVGRT
                     RSGWSYEPAQTEQGFPSGHASGPTP"
     gene            complement(6368..7477)
                     /locus_tag="P5179_06015"
     CDS             complement(6368..7477)
                     /locus_tag="P5179_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94809.1"
                     /translation="MARIIRMKRLEETVWRSGGSGDNWHMTWADNDKVYAGLCDGRGW
                     DKVPGYTGQDYNTRVYAINGAPPHHSFEHLPGFPDLLTEPLPNRNRYYGFGILALDGA
                     IYHFLSTPNRPFEEPEPRFMGIKLIYSPDNGGTWRNQDGGPVRWEPWEERSRDNMLFF
                     NEPNESFQLITVLQMGQNYRHNRDGFVYLYAPNGNTEGSMNQTVLCRVPRERILDRSA
                     YTFFAGRTPGGEGIWSKDIRDRVPVHTFPSGWVNTKIHPYAWHPSVVYYAPLGVYLMA
                     NWGMGCAPDGMWFGKPSYLGFWTSPTPWGPWEQVHEETAWVPAGDEKARAYQPQISPK
                     WIAPDGSSFWMVWTDFQSGGMYYCFNTQKVEVTIG"
     gene            8091..>9695
                     /locus_tag="P5179_06020"
     CDS             8091..>9695
                     /locus_tag="P5179_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRS94810.1"
                     /translation="MRRGIFARFARVAPVQTTDRGTSNENERRLVAFCTKPLRDAFAE
                     LDTSPRGLSSEQAEHRLEEFGANELPDRHRPGLWVDIFNRLKSPLVIQLLVIASVSAL
                     IGETKSTIIVSAMVFLSVGLSYALDQRSSRAVESLGKRVQSRTFVLRDGLEKEIKSSE
                     IVPGDVVLLHAGTIVPADLRLIVAKDFFVSESSLTGESMAVEKTVASAGSSVQSALEM
                     PNACFLGTSVTSGTARGLVVNTGSRTLFGAISARLSERSEETSFDRGVRSFTWLMIRF
                     MVVMVCVVFFIVGLTKGNWIEALLFGLSVAVGLTPEMLPMIVTVNLAKGALTMAKKKV
                     IVKRLPAIQNFGAIDILCTDKTGTLTQDRVVLERHVDVLGKTTEDVLNYAYLNSYFQT
                     GLRNLIDRAVIDSADLDADLKCRLVDELPFDFQRRRMSVVVDYEGDHVLICKGAVEEI
                     YACCGSYQIDDEVYPLIDMIRTDLFEEVERLNRDGFRVLGIAYREFPRTKTVFTVADE
                     SDLILLGYIAFFDPPKPSATEAIELLK"
BASE COUNT         1877 a         2889 c         2979 g         1950 t
ORIGIN      
        1 caggaatgcc tgccgcactg cgtgtggcct gtcccaccca ggttggcggc atggttcccc
       61 gccggggcgg gccgccgcac tccaaagaca gaaacggaga ccaccccatg ctgaactacc
      121 ttaccggaca gccgcttgaa atcgggcccg gcccgcacct gtttctggac gaccacctca
      181 ttgaggaccg gcgccagctt tcccgccgcg tgaacccgcc gtaccgccac ccgggcaacc
      241 cgatcattgt ggcggaccgg ccgtgggagg agcgcccgta ccgcccgcag gtcatccgca
      301 acccggagac gggcggctgg cggatgtact acatgtgttt cagcgggacg aactactggt
      361 cgcgaaaagg gccttcgtat tacgcgtgtt acgccgaaag cgcggacggt atccgctggg
      421 aaaagccgga gcgggctgac ctgccattcg acccctggcc cgcgacgaac gttctgcacg
      481 tggacgacga gcgggacgcg cggattcagg cgccgtgggt gtttttcgat ccgcacgata
      541 ccgacccggc ccacgcgtgg aagatgatct acaactccgc ggggctgcgg ctggcgtatt
      601 cgccggacgg gattcactgg actccggcgc gtccggagcc gctgtttgcc taccacagcg
      661 acacgatgaa ccatgtgctg tggaacgagc aggcgggcaa atgggtgctc tacatgcggc
      721 cgccgatgtt ctcctcgggc cggcatgaag gccccggcgt ggagcaccac cgccggcgca
      781 cggcggtgtc cttcagcgac gacctgctgc actggtcggt gccgcgcacg gtgctgtacc
      841 cggatgaact cgacccgacg ccgcatatag attcggtgca cgtgttcccc tatgcggggc
      901 actacatcgc gttcgtcacc atgctggacg ccaccaccgg cggcacgaac gagctgatgc
      961 tggcaagcag ccgtgatggc ctcgaatggg aaaggccgct gccacggcaa gtctggctgt
     1021 cgcgcggacg cgagggcgat ttcgacggcg ggtgcgtgtc gataggctcg gacccgatcc
     1081 gcgtcgggtg ggagctctgg ttcccgatgt ccgggttccc catgccgcag aaggtgttcg
     1141 agcaggagag cggaatcggc atcgtgaaga tgatggtgga ccgattcctg tcgcttgagg
     1201 cgccggaacc gtctctgggc ggcccggagg acttcgggtg gctgctgacg aaggagttca
     1261 tctggcgcgg gaagacgctc accatcaact gccggatgaa caacggcgat tcccggacgt
     1321 acggcgacct gcgggtggag gtggtgaagg ggccgaacga cgccgacccc gccgggcgca
     1381 tggggcaggt tgtgccgggg cacggggtgg aggactgcga cctcatccgc tcgaattcgc
     1441 cggtgcagac agtgacatgg aacggcaatc cggaccttgg atttctggat ggccagccgt
     1501 tgtatcttcg cttccgcatc cgcaacggcg gcctgttcgc gttccgcatg ggggagtgag
     1561 gcggaacatg agccgttgac cactaccggg cacaaagcgt gtcgggtggt tattttcaag
     1621 agggaaggtg cggagcgcca ggtgccgggg gtcaaccatg gaagcgacag atgcacggaa
     1681 acgagctgct tacttttccg cagccctgcg gaaaaggctc ggcgcgaatc tgcggcaggt
     1741 gatcctgttc ggttcgcgtg cccggggaga cttccgcgag ggttctgact acgatttcgc
     1801 agtggtggta cgccggaagg accccaccgt ccagtcctct attgttgagg caggtgttga
     1861 tttcctgaat cgcttcgatg aggtttccgc ctgtgtggtg tacgacgaga ccgagtgggc
     1921 cgagagcacg cgattgcccc tcggagtgaa tatcaagcgt gagggaattg cgctgtgaag
     1981 gatcagaaaa acatccggga aattctggca aaggcgcacg agaaacttga ctccgcgaac
     2041 gtgctttttg agcaccagca gtatgacgat gccatttccc gggcgtatta tgcggtcttt
     2101 cacgcgatga ctgcggccct cttcttccag gatatggtat ttcgtcccac gcccagacaa
     2161 ttggcgcatt caaccgcgaa ttcgtgaaaa gtggtgtgtt cccggtcgcg tttaccacca
     2221 ccatccagtc cctttttcag aacaggcagg tggccgacta cggcgcggga aaagccgccg
     2281 atcgcgacac agcgctttcg agtctcaatg gtgcaacggc ggtagtaaca gcaattgaga
     2341 agtacctcgc ggacgcgaac gcgctttgac tcacaacgag gaatagcccg gtgtcagccc
     2401 ccaaacgcgt tctcatcctc caaccgattc acgacgccgg tctcgcggcg ttgcggcagg
     2461 ccggcttccg cacgcgttcc tctgtcgccc cttcgcgggt ttgcgcctcc gcaaacgcgt
     2521 ccgattcttc gtacctttct gttaactcaa gcgccaaatc caggtacgct tgagcgggtg
     2581 cagaagagag gtactaccat ggctcttgaa ctgccgctcg tcaagattcg agacatctgc
     2641 gcacgctacg gcgtaaccga actctgcctg ttcgggtctg cggcacgtga tgatttttca
     2701 ccggaaagcg acgtggacct gctcgtaacg ttccgaccgg atgcgacggt aaccttcttc
     2761 acgctttcgc gcatggccgc cgagctctcc gcggtaatcg gtcgccccgt ggaccttgtt
     2821 cccaagacgg atctgaagcc gcttctgcgc gaggaggttc tcgcgacgac cagggtgtta
     2881 tatgcggcgt gaccggttgt tcctcgcgga catctgctcc gctgctgatg acatcagtgc
     2941 gttcctcgaa ggggtatccc gtgacgcgtt tctgacgaac aaaatgctcc aaagcgccgt
     3001 tcttcagaaa ctcattgtca tcggcgaagc ggctgcaaga gtctcgcccg agcttcgtgc
     3061 gcgacatccc ggtgttccgt gggctgaaat gacagcgttc cgcaacatcg cagttcacgc
     3121 gtatttcagc atcaactgga gcattgtgtg ggcaactgct cggatcgatg ttccccaggt
     3181 gaggaacaac gtgcaacgag taatggaaaa cgaggaatag cccggtgtca ggccccaaac
     3241 gcgttctcat cctccaaccg attcacgaag ccggcctgac ggcgctcagg aacgccgggc
     3301 tggacatcat ccatgcgccc gacgcaactc gcgaaacgct gctgagtctg atcccggagg
     3361 cggacgggct cattgtccgc acgacggcat accgcattga cgcggaggtc atggatgctt
     3421 cccggcggct gaaggtgatc ggccggcacg gggtaggggt ggaccacatt gatctcgcgg
     3481 ccgcggaagc ccggggaatc accgtggtga acacgccgct ggccaactcg cagggcgtgg
     3541 ccgagtacac gatcgggctg atgctttccg cctgccgttt catcccgagg gccgaccggg
     3601 cggtccgcga agggaactgg ccggagcgag aacggctggt gggcgatgaa ttgttccggc
     3661 acactctcgg aataatcggg ctggggcggg tcggcacgcg tgttgcccgg attgccgcgc
     3721 tggggttcca gatgcgcgtg ctctacttcg acatcatccg caagcacgag accgaggcag
     3781 cgctcggggt ggaattcgcg ccctttgagg aggtcctgcg ccaggcggat tttctcacgc
     3841 tgcacacgcc tctcaccgag gcaacccgcg ggatgatgaa cgaggccacg ctggcgctta
     3901 tgaagcaggg cgcgtatctc gtcaatgcat cccggggaga agtggtgaag attgacgcgc
     3961 ttgcccgcag catcgccaca gggaaaatcg ccggggcggc gctggacgtg ttcccggatg
     4021 agccgtttcc ggccgaccac cccctgctgg cgatgccgga agtgatcgtc agcccgcaca
     4081 tggcttcgca ctccagggac agcatggtgc gcatggctct cgtggccgag gatgtcgcgc
     4141 gcgttctcaa cggacaggag ccgctccatc ctgtgggaac cgggaccgtt tcgtaagtat
     4201 ctttctctgc gctttcttcc ccggcgcatg ccaaccgcac aggagaacca actcatgccg
     4261 cagaaaatca tgagcgagat ctgggactac atcgctcagg aattccgtga taccatttcc
     4321 cgcgcgacac cggagcaact cggctggcgc atcacgccga agacgtattc gttcgcggag
     4381 actgcgtggc gcgcgctcgg agcagcgtac ctctggtctg tcgtgctgag cggcgcgaaa
     4441 acactggaag aggcgatcac catggacccg gccgcgctgg atcttctcca ggccgcgctg
     4501 aacaagtacg ctcccggcca tttcccggac agcatgccct gcgatcagga cactctcctt
     4561 gagcggacag atgaggtgat cgccctcctc agcgctcaat tagtggagat gagtccctcg
     4621 atgcgcacga aggatttcag gacgtggtgg gacgctgtgt atacggggga cgagatagtt
     4681 gcgcgtttga tgtggcgggt ggcgcatttc aacgggcaca tgcacagcat cctgctccac
     4741 gcaaggggat aacaccgtgg gacgtaccgg ctggctgctc atggtcgtcg ccatgtgggg
     4801 gacggcgaac ttcctcatga aagtggtcgg ctcccgtatt tcaagcgcca gcggggtgct
     4861 gggaatcgtg atcgggtact ccgctatcgg cctctggatt gctctctccg gcggcggcag
     4921 gattggcacg ggagtcggtt acctcggcgc actcgccgtc ggcgcccttt acattggcgg
     4981 caactacgcg tatctgcggc tggcacagcg ggaagacatc agcgcaatcg cgccaatcgg
     5041 cagcctgaat gtggtgattc cggtcgcgct cgggtttctc cttctcggcg agccgatgac
     5101 attgcgaaag ctggtcgggt tcgcgtgcgc ccttgccgct gttgtgctgt tgcggtaggg
     5161 tgcgggttgc ggcatgcctt ttgacctcca ccgccagttg caggagcttt acgccggcga
     5221 agatggtcag cgcgaagtac gaatcaatgg ctcgttcgtc gccgacgtgg tgcggcacgg
     5281 agtcatctac gaaatacaga cacgcaagct gagcaacatc aagagcaaga tcacgtccct
     5341 cctcgaaaca acgccgatcg tcattgtcta ccccgtttct gttcacacca ccatcgtcca
     5401 ttgcgatggg gcggaccttc gcgaatgtga ccggcgccca tcccccaaac agggtcaggc
     5461 gctggatgcc ttcggcgaga ccgtgtatct cgcacacctg cttgcgcacc ccaacatggc
     5521 gcttgagata cttctggttg acacagagga tatccgcatc aagggggacc ctcacgcggc
     5581 aaccagaaga agacggcgac gacgcagaca ttcagaatgg accacggtaa accgccgtct
     5641 taccgccaca catggacgcg tccgactgga tactcccgcg gacgggcttc agcttctgcc
     5701 tcctgccgtg aaattccctt tctgcacccg tgatctcgcg aacgcaacag gggcctcgct
     5761 ctcccgcgcc cagcagatcc tttacacact tttccacatg ggcgccctgc gccgcgtcgg
     5821 gcggacacgg tccgggtgga gttacgaacc ggcgcagact gaacaaggct ttccgtctgg
     5881 acacgcttct ggacccacgc cgtaaatcac tgcacagccg ggtattacag agcctgacga
     5941 gcgctgtcca ccagttatcc cccagggtgg agagttcagg tgcagagagg tcaacaactc
     6001 cacatttgac cccacttttt caccacagcg acaaagttat tcaccactta tccaccagtt
     6061 ttcctaggat tggggctggt ttccaaagta ttgttcttat tcgctttaaa tcaagtcggt
     6121 tctcgtatct aatgcgttgt aaaccacact tactactaag cacatgcacc agaaatcctg
     6181 acttgtcagg tagtgttttt tgtggcgccg tgtttcatgt tcgtgccact gtcctcagct
     6241 cgtcgaaacc gaggtggtca accacaggga caatgctacc ggtatggcgc cggcaaggcg
     6301 ggagtgtgtg atgggcgtcc attaccactg tgggcacaaa tggcgaggga aaaaggggct
     6361 tccgcgctta cccgatggtg acctccacct tctgcgtgtt gaagcaataa tacatgccgc
     6421 ctgactgaaa gtctgtccac accatccaga aagagctgcc gtcgggcgcg atccatttcg
     6481 gggagatctg cggctgatag gcgcgcgctt tttcgtcacc ggcaggcacc caggcggttt
     6541 cctcgtgcac ctgttcccac ggaccccacg gggtgggtga tgtccagaac cccagataac
     6601 ttggcttgcc gaaccacatg ccgtcgggtg cgcaccccat gccccagttg gccatcagat
     6661 agacaccgag cggcgcatag tagacgacac tcgggtgcca cgcgtacggg tggatcttcg
     6721 tgttcaccca gccggagggg aacgtgtgaa ccggcacacg atcgcgaata tcctttgacc
     6781 atatcccttc gccacccgga gttcgtcctg caaagaatgt gtaggccgaa cgatccagaa
     6841 tacgttccct gggcacgcgg cagagcaccg tctggttcat tgagccctcg gtattgccgt
     6901 tgggtgcata gaggtagacg aacccatccc ggttgtgccg gtagttttga cccatctgaa
     6961 gcaccgtgat gagctgaaaa gattcgtttg gttcattgaa gaagagcatg ttgtcgcggc
     7021 tgcgctcctc ccatggttcc caccgaacag gcccaccatc ctgatttcgc catgtaccgc
     7081 cgttgtcggg ggagtagata agcttgatcc ccataaagcg cggttccggc tcttcaaacg
     7141 gccggtttgg tgtgctgagg aagtggtaga tcgcgccgtc aagggcgagt ataccgaacc
     7201 cgtagtagcg attacggttg ggcaatggtt cggtgagaag gtcggggaac cccggcaggt
     7261 gctcgaagga atggtggggc ggcgctccgt tgattgcgta cacccgggtg ttgtagtcct
     7321 gccccgtgta gccgggcacc ttatcccacc ctctgccgtc acacagaccg gcgtagacct
     7381 tgtcgttgtc ggcccaggtc atatgccagt tatcacctga gccgcctgaa cgccagacag
     7441 tctcctcgag ccttttcatg cgaatgatgc gggccacagg gtggttcctt ctgcgagagg
     7501 tgagccttgc gcgtgaagaa cgatgcacac tcaagcatcg cgctttggcc gcgtaagctc
     7561 aaccaccgtc tgcgcgcgtc attgtgtgcg tggtggccgt ccaccgctcc ggggaagcgc
     7621 accagcgacg cttgcggcgc aacacggtag cgagctattt tcaaaagtaa ttcgcgctgg
     7681 agcccggcgc tccaggagat ttctccttgt cgtgaggagt atatgagcag cccggacggc
     7741 aatagtagcc cgagtatcat tcaatccgcc gcgatcgagg aacaacaggc cgacgttcct
     7801 cctttgcggg cgtgggcggc agcggtggcg tacccatttc tcctctgatg acgtgaaacg
     7861 gtgttcgcct ctgaccgctg gtgcccctgt gcggtcaaaa agaggcgaac tatggcatat
     7921 catccgttca atctctcttc ctacaagtcc ttcctacctc accgaactgc accatccctg
     7981 cagaactgca gggtagttcg cgttgatgcg gcgctgtcgt ttcgccgcag atttcccgca
     8041 ttccttgtgt ccgacccctc ccgacgcctt cacacatgga ggagtgcgac atgagacggg
     8101 ggatctttgc gcggtttgcc agggtagccc cggtgcaaac gactgaccgc ggtacgtcga
     8161 acgaaaacga gagacggctg gtcgcattct gcacgaaacc ccttcgggac gccttcgccg
     8221 aactcgacac atctccccgc gggctgagct cggaacaggc ggaacaccgc ctggaggagt
     8281 tcggtgccaa cgaactgccg gaccgccatc gccccggtct gtgggtggat atcttcaacc
     8341 gcctgaaaag cccgctggtc atacaacttc tcgtgatcgc ctccgtttcc gctctcatcg
     8401 gggaaacgaa gtccaccatc atcgtaagcg ccatggtttt cctcagcgtc gggttgtcct
     8461 acgcactgga tcagcggtcc agtcgagcag tggaatcgct tgggaagcgc gtgcaatcac
     8521 gcacctttgt gctgagggac gggctggaaa aggaaatcaa gagctccgag atcgttcccg
     8581 gcgacgtggt cctcctgcat gccggcacca ttgttcctgc tgatctaaga ctgatagttg
     8641 ccaaagactt cttcgtcagc gagtcgagtc tgaccggcga gtccatggcc gttgagaaga
     8701 cggttgcaag cgcgggctcg tcggttcaat cggcgttgga gatgccgaac gcctgttttc
     8761 ttggcacctc cgtcaccagc ggcacggcac ggggactggt ggtcaacacg ggctcacgga
     8821 cgttgttcgg ggcgatttcc gcgcggctct ccgagcggag cgaagaaacg agtttcgacc
     8881 ggggcgtgcg ctcgttcacc tggctgatga tccggttcat ggtggtgatg gtgtgcgtgg
     8941 tgtttttcat cgtcggtctg accaaaggca actggattga ggcgctgctc ttcggacttt
     9001 ccgtcgccgt cgggctcacc ccggaaatgc tcccgatgat tgtcaccgtg aatctggcca
     9061 aaggcgcact cacgatggcg aagaagaagg tcatcgtgaa gaggctcccg gcgattcaga
     9121 acttcggcgc cattgatatt ctctgcacgg acaaaacggg aaccctgacg caggatcggg
     9181 tggttcttga acgccatgtg gatgttctcg gaaaaacaac cgaagacgtc ctgaactatg
     9241 cgtatctcaa cagctacttc cagaccggac ttcgcaacct gatagatcgc gcggtgattg
     9301 actcggcgga tctggacgcg gacctgaagt gccggctggt ggacgaactg ccttttgatt
     9361 tccagcgccg gcgcatgtcc gtggtggtgg attacgaagg ggaccacgtt ctgatctgca
     9421 agggcgcggt ggaggagatc tacgcgtgtt gcggcagcta tcagatcgac gacgaggttt
     9481 acccgctgat cgacatgatc cgtacggatc tcttcgagga ggtggaacgg ctcaaccgcg
     9541 atggattccg ggtcctcggc atcgcctacc gcgaattccc gcgcacgaag acggtattca
     9601 cggtagcgga tgaaagcgat ctcatcctgc tcgggtatat tgcgttcttc gacccgccga
     9661 agccctcagc gacggaagcc atcgagctgt tgaaa
//
LOCUS       DAOTNG010000093         9646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6510_length_9646_cov_5.528725, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000093 DAOTNG010000000
VERSION     DAOTNG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_9646_cov_5.528725"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1577)
                     /locus_tag="P5179_06025"
     CDS             complement(<1..>1577)
                     /locus_tag="P5179_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS94811.1"
                     /translation="LEKGDLISEEQVIDLEERGFQFSAEMGAGAIKKMLADLNLDELS
                     TELRETIRKETSAQRKAEALRRLSIVEAFRQSNNRPEWMILDVIPVLPPELRPLVPLE
                     GGRFATSDLNDLYRRVINRNNRLKKLIEIKAPEVVLRNEKRMLQEAVDALFDNGRRSR
                     AVRGDANRALKSLGDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLYECGLPKIM
                     ALELFKPFIIHELERRGLVQTVKSAKKKIEQGSPEVWDILEEVIQGHPVLLNRAPTLH
                     RLGIQAFQPVLVEGKAIRIHPLVCKAFNADFDGDQMAVHVPLSFEAQIEASVLMLSAN
                     NLLLPSSGNPVTIPTQDMVLGCYYLTKRLPAGSKKRRVGRFSSPRDAILAYDLGKYDI
                     HDDVEVRINGKFITTTFGRLLFNEILPPEIGFVDKLMGSKELTALVREVFRKLGNTRT
                     VEVLDQLKKTGHYYATKAGISIGIDDVVVPAEKDRLIQDAMQQSELVQEQYEQGVITE
                     GERYNKIVEIWSHTSQR"
     assembly_gap    1578..1677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1678..2169)
                     /locus_tag="P5179_06030"
     CDS             complement(<1678..2169)
                     /locus_tag="P5179_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS94812.1"
                     /translation="MPQQRKPLDFDSIKIQLASPETIRSWSYGEVLKPETINYRTYKP
                     ERDGLFCERIFGPTRDWECHCGKYKRIRYKGVICDRCGVEVTQAKVRRERMGHIELAV
                     SVSHIWYFKSVPSRMSYLLAMTVRNLERVLYYESYVVLDPGNAPLEKGDLISEEQVID
                     LEER"
     gene            complement(2260..6054)
                     /gene="rpoB"
                     /locus_tag="P5179_06035"
     CDS             complement(2260..6054)
                     /gene="rpoB"
                     /locus_tag="P5179_06035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRS94813.1"
                     /translation="MPERMIKRRSYAKLPTILDIPNLLELQVASFEDFLQANTPPEKR
                     KIKGLQEVFESVFPITDVHNLYSLEFVSYSIGRPKYDIDECRDRGATYAAPLRAVLRL
                     VTREKGDEKRVKDVIEQVVYLGDLPLMTSTGTFLINGAERVIVSQLHRSPGVFFDEVA
                     HPNGKRLFTARIIPYRGCWVEFTMDINDVMYVHIDRKRKQPVTLLLRALGYSSDLDVL
                     NLFYERITTKLSSKVKGRLLAADVVNAATGEVLADAFATLTDETIAMLEEHGVEEVEV
                     YSPNTEDEIGIIENTLRRDPCQDERGEEDALYRIYQLMRPGDPPNVETARTFVERLFF
                     SPKRYDLGDVGRYRMNQKLDLKIPQDTHVLTREDFISIIRYIIALRNGSPSAQTDDID
                     HLGNRRCRTVGELLANQFSIGLSRMARTIRERMSLRDNENLTPQELVNAKTVSTVIAS
                     FFGSSQLSQFMAQTNPLDELTHKRRLSALGPGGLTRERAGFEVRDVHHTHYGRVCPIE
                     TPEGQNIGLIMSLAAHARINQYGFIETPYRVVKDGVVTTEIRYLSSDEEDRYVIAQAN
                     APLKEDGSFANNLVKARRRGDFPTVSPLEVDYMDVSPIQLVSAAAALIPFLEHDDANR
                     ALMGSNMQRQAVPLLKAEAPLVGTGIERRIALDSGAVTLAKADGEVVYVDSSKIVVRE
                     KKPVHANPLALDEGHERKYSLMKFQRSNQDCCQNQRPLVRPGDKVKAGEVLADGPATD
                     RGDLALGCNVLCAFMPWHGYNFEDAIVVSERLIRNDVFTSVHIEEYELQVRDTKRGQE
                     ELTRDIPNVSDQALRDLDENGIVRVGARVKPGDILVGKVTPKGETELSPEERLLRAIF
                     GEKAGDVRDASLKAPAGMEGVVIDTKIFSRKERDEATRSRNKETIEQIRANMDERAAR
                     VRSALKQRSIAVCQGLTAGNWRSEADGTIVVHEGTVLTEEILSELDLTTLRPDTTWTT
                     DSAANGKIEEMVRYAHDMLAAIQEEFDRESEKVVRGDELPPGVIQLVKVYVAHKRKLS
                     VGDKMAGRHGNKGVVAKVVPVEDMPYLPDGTPVDLVLNPLGVPSRMNLGQILETHLGW
                     AAEKLGIHVSCPVFDGATVAEIKNALKEAGLPESGQTVLYDGMTGERFEREVTVGYIY
                     MMKLIHLVADKIHARSIGPYSLVTQQPLGGRAQFGGQRFGEMEVWALEAYGASHLLQE
                     MLTVKSDDVPGRSRIYEAIVKGENPPEPGIPESFNVLVKELQALCLDVVLQQDGQVAD
                     QS"
     gene            complement(6224..6607)
                     /gene="rplL"
                     /locus_tag="P5179_06040"
     CDS             complement(6224..6607)
                     /gene="rplL"
                     /locus_tag="P5179_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRS94814.1"
                     /translation="MADLQALKTQLSALTVLEIAQLTKELEAEWGVSAAAPVAVAAAP
                     AAGGAAAAAPVEEQTEFTPTLVDVGSQKIQVIKVVRELTGLGLKEAKALVDAGTAPVK
                     EGVSKEEANTIKEKLEAVGAKVEIK"
     gene            complement(6623..7213)
                     /gene="rplJ"
                     /locus_tag="P5179_06045"
     CDS             complement(6623..7213)
                     /gene="rplJ"
                     /locus_tag="P5179_06045"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRS94815.1"
                     /translation="MPTPKKNETIARLTDMFNSANGIFLTDSTGLKVELVTNLRKRCA
                     AENIGFMVVKNTLARRAAKAAGLPDLDKWFTGPTAVAYSKNNPAGPVKILRAFVREVR
                     EANGKLEIKKGVVDGQVIDDAQMQMLADLPTPEVVRARFLGLLQAPASQFVGILSAGP
                     ASLVRALDQRRQQLDEQAAGSTAGSTEAPTDAPAGQ"
     gene            complement(7236..7940)
                     /gene="rplA"
                     /locus_tag="P5179_06050"
     CDS             complement(7236..7940)
                     /gene="rplA"
                     /locus_tag="P5179_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRS94816.1"
                     /translation="MPKLAKKYQHDLEKVDRTKSYGLREAIEVIKQFEPRGFDQTVEG
                     VMKLNVDPRKADQLVRGSVVLPSGTGKTVRVLVFARGEAATAAEQAGADFVGADDLVA
                     KIQEGWTDFDVAIATPDLMGVVGRLGRVLGPRGLMPNPKTGTVTQDTAAAVQAAKGGK
                     IDFRVDKAGNVQAPLGRLSFSVDALYANAQVFFEAVMRARPTAVKGQYVHSANLCASM
                     TPSVPLDRNQFQSLVK"
     gene            complement(7952..8389)
                     /gene="rplK"
                     /locus_tag="P5179_06055"
     CDS             complement(7952..8389)
                     /gene="rplK"
                     /locus_tag="P5179_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRS94817.1"
                     /translation="MAKKVIAQIKLQLPAGQATPAPPVGPALSQHRLNIMEFVKAFNE
                     RTRSQQGMIIPAVITAYADSSFTFILKTPPAVTLLKKAAGLEKGSPEPNAKKVGKVTW
                     DQVVEIAKTKLPDLNTTNLDSAASMIAGTARSMGLEVVGRPTK"
     gene            complement(8485..9069)
                     /gene="nusG"
                     /locus_tag="P5179_06060"
     CDS             complement(8485..9069)
                     /gene="nusG"
                     /locus_tag="P5179_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRS94818.1"
                     /translation="MTDTPQGEAPRPKRWYAVHTLSGHENKVLHYLKNSAEALALGDQ
                     IGEVVIPIEDVPEMREGKRVTTKRRFLPGYVLIEMELTSQSRYVVENTPGVTGFVTTG
                     GRSSKEPAPLRPSDVERILQQVERKSTAPETDSAYRPGDFVKVVDGPFSDFVGVVEAF
                     DTAKGKVTIAVSIFGRPTKVELDLLQIEPAPPKA"
     gene            complement(9073..9258)
                     /gene="secE"
                     /locus_tag="P5179_06065"
     CDS             complement(9073..9258)
                     /gene="secE"
                     /locus_tag="P5179_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRS94819.1"
                     /translation="MFQKVATYYREVVMEMGKVSWPTRDQLKNSTIVVLIVTAIFAVF
                     IGAFDWILSQIVQWFLR"
     gene            complement(9288..9365)
                     /locus_tag="P5179_06070"
     tRNA            complement(9288..9365)
                     /locus_tag="P5179_06070"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9328..9330),aa:Trp,seq:cca)
     gene            complement(9390..9542)
                     /gene="rpmG"
                     /locus_tag="P5179_06075"
     CDS             complement(9390..9542)
                     /gene="rpmG"
                     /locus_tag="P5179_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRS94820.1"
                     /translation="MPRDIITLACEDCKRRNYSGDKNKRKHSERVTYKKYCPWCKKHT
                     PHRETR"
BASE COUNT         1870 a         3072 c         2521 g         2083 t
ORIGIN      
        1 tgcgctgcga cgtgtgcgac cagatttcca cgatcttgtt gtagcgctca ccttcggtaa
       61 tcacaccctg ctcgtactgc tcctgcacca gttcggattg ctgcatcgca tcctgaatga
      121 gccgatcctt ctccgccggc accaccacgt cgtcaatgcc gattgagatt ccggccttgg
      181 tcgcatagta gtgcccggtt ttcttcaact gatcgaggac ttccacggtc ctggtattgc
      241 ccagtttcct gaacacctcg cgcacgagag cagtcagctc tttcgaaccc atcaacttgt
      301 caacaaaccc gatctccgga ggcagtatct cgttgaagag aagccggccg aacgtcgtgg
      361 tgatgaattt gccgttgatc cgaacctcca cgtcgtcgtg gatgtcgtac ttgcccagat
      421 cgtaggcgag gatggcgtcg cgaggtgaac tgaaacgtcc gacccgacgc tttttgcttc
      481 ctgcggggag ccgcttcgtg aggtagtaac atcccagtac catatcctgc gtcgggatgg
      541 ttaccgggtt gccgcttgaa ggcagaagca ggttattggc cgaaagcatc agcaccgacg
      601 cctcgatctg cgcttcaaag gaaagcggaa catgcaccgc catctggtcg ccgtcaaagt
      661 cggcattgaa cgccttgcag accagcgggt gaatcctgat cgctttacct tcgacaagca
      721 ccggttgaaa cgcctgaatc ccgagacgat ggagcgtggg cgcacggttc agaagcaccg
      781 gatgcccctg aatcacctcc tcgaggatat cccacacctc tgggctgccc tgttcgatct
      841 tcttcttcgc gctcttgacc gtctgcacca gcccgcgccg ttcaagctcg tgtatgatga
      901 acggcttgaa aagctcaagc gccatgattt tcggcaaccc gcactcataa agcttcagtt
      961 ccgggcccac cacgatgact gaccgacccg agtaatcaac acgcttcccc agaagattct
     1021 gccggaatcg gccttgcttg cctttcagca ggtcgcccag gctcttcagt gcccggttcg
     1081 cgtcgccacg caccgcgcgg ctccgccgcc cgttgtcaaa cagcgcatca acggcttcct
     1141 gaagcatccg cttttcgttg cggagaacca cctccggtgc cttgatttct atgagcttct
     1201 tcaaccggtt gttacggttg atcacccgac gatagagatc gttcaggtcg ctcgtcgcaa
     1261 aacggcctcc ttcaagcgga acaagcggac gcaattccgg cggaagcacc ggtatcacat
     1321 ccagaatcat ccactcgggc cggttgttcg actggcggaa tgcctccacg atgctcaggc
     1381 ggcggagggc ctcagccttc cgctgggcgc tcgtttcctt gcgaatcgtc tcccgaagct
     1441 ccgtcgagag ctcatcgagg ttcaaatccg ccagcatctt cttgatggcg ccggcgccca
     1501 tctccgccga gaactgaaaa ccgcgttcct caaggtcaat gacctgctct tccgagatca
     1561 gatcaccctt ctccagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     1681 ttcctcaagg tcaatgacct gctcttccga gatcagatca cccttctcca gaggcgcatt
     1741 tccgggatcc aggacaacgt aagactcata gtacagcacg cgttccagat tccggaccgt
     1801 catcgcgagc aaatagctca tacgggacgg aaccgacttg aagtaccata tgtgtgacac
     1861 agacacggca agctcaatgt gtcccatgcg ctcacgccga accttggcct gcgtaacctc
     1921 aacgccgcac cggtcgcaga tcacaccttt gtaccggatg cgcttgtatt tcccgcagtg
     1981 gcattcccag tcacgcgtgg ggccgaaaat ccgctcgcag aaaagcccat cgcgctcggg
     2041 cttgtacgtg cgatagttga tcgtttcggg cttcagaacc tcaccgtacg accagctccg
     2101 aatcgtctcc ggtgaagcga gttgtatctt tatcgaatcg aaatcgagcg gcttgcgctg
     2161 ctgcggcaaa cctgcccgcg acagcagttg cgacgccaaa acgtccggtt gttcgaccac
     2221 ggccgttctc ctctcgttac gagggttagg gtagaggttt cagctctggt cggccacttg
     2281 accgtcttgc tggagcacga cgtcaaggca cagtgcctga agctctttca caagcacgtt
     2341 gaacgattcc gggatgcccg gctccggcgg attctctcct ttgacgatcg cttcgtagat
     2401 ccgcgaccgc cctggaacgt cgtccgactt caccgtcagc atctcctgaa gcagatggga
     2461 cgcgccgtat gcttcaagcg cccacacttc catttctcca aaacgctggc cgccaaactg
     2521 ggcccggccg ccgagcggct gctgcgtgac gaggctgtac gggccgatgg aacgagcgtg
     2581 gatcttgtcc gcaacaaggt ggatcagctt catcatgtag atgtaaccta ccgtcacctc
     2641 gcgctcgaat ctctcgcccg tcataccgtc gtacagaacc gtctgtcccg attccggcaa
     2701 accagcttct ttcagcgcat tcttgatttc cgcaacggtc gcgccatcaa acaccgggca
     2761 cgacacgtga attccaagct tctccgccgc ccagccgaga tgggtctcga gaatctgccc
     2821 gagattcatc cgcgagggca cgccgagggg attgagaacc agatccaccg gggtgccatc
     2881 cggcagatac ggcatgtcct ctaccggaac cacctttgca acaacaccct tgttgccgtg
     2941 ccgtcccgcc attttgtccc cgacggagag cttgcgcttg tgcgcgacat acaccttgac
     3001 aagctgaatc acgcccggcg gaagctcgtc tccgcgcacc accttctcgc tctcgcgatc
     3061 aaattcctcc tgaatcgccg caagcatgtc gtgcgcatac cgaaccattt cctcgatctt
     3121 gccgttggcc gccgagtcag tcgtccatgt cgtatccggt cgaagagtcg tcagatcgag
     3181 ttctgaaagg atctcctcgg tcaggacggt cccttcgtga accacgatcg ttccgtctgc
     3241 ctcggagcgc cagttgcctg ccgtcaaccc ctggcagacc gcaatggacc gctgcttcaa
     3301 agcgctccga actcgtgcgg cgcgctcatc catgttcgcg cgtatttgct cgatcgtttc
     3361 cttgttgcgg cttcgcgtgg cctcatcgcg ctctttgcgg gagaatatct tcgtgtctat
     3421 gacaacgcct tccatgcccg ccggggcctt cagcgacgca tcacgcacgt cgccggcctt
     3481 ctccccgaaa atcgcccgca aaaggcgctc ctcgggcgag agttccgttt cgcctttcgg
     3541 cgtcaccttc ccgaccagga tgtctccggg cttcactctc gcgccaaccc ggacgatccc
     3601 gttctcatcg aggtcacgca gcgcctggtc actcacgttc ggaatatccc gcgtgagctc
     3661 ttcctggcca cgttttgtgt ctcggacctg aagctcgtat tcctcgatgt gcacagacgt
     3721 gaagacgtcg tttctgatga gccgctcgct caccacgatg gcgtcctcaa aattgtagcc
     3781 gtgccacggc atgaatgcgc acaaaacgtt gcaacccagc gccaggtcac ccctgtccgt
     3841 cgcagggccg tccgcgagca cctcacccgc cttcaccttg tccccgggcc gcacaagtgg
     3901 acgctggttc tggcagcaat cctggttcga ccgctggaac ttcatgaggc tgtatttgcg
     3961 ctcgtgacct tcgtccagcg ccagaggatt ggcgtgaacc ggcttctttt cgcgcacgac
     4021 gatcttcgac gagtccacgt acaccacctc gccgtcggct ttcgcaagcg tcaccgcgcc
     4081 ggaatcgagt gcgatacgcc gctcgattcc cgtcccgaca agcggcgcct ccgctttcag
     4141 aagcggaacg gcctgccgct gcatgttgga acccatcagt gcacggttgg catcgtcgtg
     4201 ctcgaggaag ggaataagcg ccgccgccgc gctgacaagc tgaatcggcg acacatccat
     4261 gtagtccact tccaggggac tgaccgtggg gaaatctccg cgccgacgtg ccttcaccag
     4321 attgttggca aaggacccgt cttccttcaa cggcgcgttc gcctgcgcga tcacgtaccg
     4381 gtcttcctca tccgaagaca gataccggat ttcggtcgta accacaccgt ctttcaccac
     4441 ccggtacggc gtctcaatga acccgtactg gttgattcgg gcgtgcgccg caagcgacat
     4501 gataagaccg atgttctggc cttccggcgt ctcgataggg cacacacggc cgtaatgagt
     4561 atggtgaacg tcacggacct cgaacccggc gcgctcccgc gtaagaccgc ccggcccgag
     4621 agcactcagc cgccgcttgt gcgtaagctc gtccagaggg ttcgtctgag ccatgaactg
     4681 gctgagctgg ctggaaccga aaaaggacgc aatcaccgtc gaaacggttt tcgcgttcac
     4741 cagttcctgc ggcgtcagat tctcattgtc ccgaaggctc atccgctcgc ggatggtgcg
     4801 agccatgcgc gaaagcccga tcgagaactg gttcgcaagc agctcaccca ccgtccggca
     4861 ccgacggttc ccgagatggt cgatgtcatc ggtctgcgcg cttggtgatc cgttccgcag
     4921 cgcgatgatg taccggatga tcgaaatgaa gtcctcacgc gtcaggacat gcgtgtcctg
     4981 aggaatcttg agatcgagct tctgattcat ccggtaccgg ccgacatctc ccagatcgta
     5041 ccgtttcggg ctgaaaaaga gccgctcgac gaacgttcgc gcagtttcca cgttcggcgg
     5101 atcgcccgga cgcatcaact gatagatgcg gtaaagggcg tcttcttccc cgcgttcgtc
     5161 ctggcagggg tcacgccgca acgtgttctc gataatgccg atttcatcct cggtgttcgg
     5221 actgtagact tcaacttcct ccaccccgtg ttcctcgagc atcgcgatcg tctcgtcggt
     5281 aagggtggcg aacgcgtccg ccagaacctc tcccgtcgcg gcatttacca cgtccgccgc
     5341 caggagccgc cctttcacct tgctcgaaag cttggtcgtg atccgttcat agaacagatt
     5401 gagcacgtca aggtccgagc tatatccaag cgcgcgcagc agcaacgtta ccggctgctt
     5461 ccgtttgcgg tcgatatgga catacatgac atcattgatg tccatcgtga actcgaccca
     5521 gcaaccacgg tagggtatga tccgcgcggt aaagagtctc ttgccgttgg ggtgggcaac
     5581 ttcgtcaaaa aacacgccgg gcgaccggtg caactggctc acgattacgc gctcagcacc
     5641 gttgatgagg aacgtgcccg tggaggtcat gagcggcaga tcgccaaggt aaactacctg
     5701 ctcaatgacg tccttgaccc gtttctcgtc acctttttcc cgcgtgacaa gccgcaaaac
     5761 cgccctgagc ggtgcggcgt acgtcgcgcc ccggtcacgg cattcgtcga tgtcgtattt
     5821 ggggcggcca atagaatagc tgacaaactc aagcgagtac aggttgtgaa catcggtgat
     5881 cgggaatacg ctctcgaaca cctcctgcaa gcccttgatt ttccgcttct ccggaggagt
     5941 gttggcctga aggaaatcct caaacgaggc aacctgcaac tcaagaaggt tggggatatc
     6001 gagaattgtg gggagcttgg catatgaccg gcgttttatc atccgctctg gcatggcccc
     6061 aatccgctcc ttttggcgaa agaacaaagt cgcggcaccc ttccctcaaa gaactcgttg
     6121 cccttcgtgg cgggtgccct gatgagaaca gcaaagacga cacatcatgc cgaggccgag
     6181 aatcagccga tgcgtcgcct ttgcacaagc tcgagacggg agattacttg atctctactt
     6241 tcgctccgac tgcttcgagc ttttctttga tcgtgttcgc ctcttccttg gagacacctt
     6301 ccttcacggg agcggtgccg gcatccacga gcgccttcgc ttccttcaag cccaggccgg
     6361 tcagttcgcg cacaaccttg atcacctgga tcttctggga gcctacatcc accaacgtcg
     6421 gggtgaattc cgtctgctct tcaaccggtg cagcggcagc cgcaccaccg gccgcagggg
     6481 cagcggcaac agcaacaggc gctgcagccg aaacacccca ctcagcctca agttccttcg
     6541 tcaactgcgc aatttcgaga accgtcagtg cgctcaactg ggttttcaac gcttgaagat
     6601 ccgccatgga gactcttcct ccttattgcc ctgcgggtgc atccgtcggg gcttcggttg
     6661 aaccggccgt cgatccggcc gcttgctcat caagttgttg tcggcgctga tccagagcac
     6721 gcaccagcga agccggtcca gccgacagta taccaacgaa ctgactggcg ggagcttgca
     6781 gaagcccgag gaatcgcgcg cgaacgacct caggcgtcgg aagatctgcc agcatctgca
     6841 tctgggcgtc gtcaattacc tgaccgtcaa ccacgccctt tttgatctca agtttgccgt
     6901 ttgcctcgcg gacttcgcga acaaacgctc tcagaatctt taccggacct gccgggttgt
     6961 tcttcgaata cgcaacggca gtcggtccgg tgaaccactt gtcaaggtcg ggcaaacccg
     7021 ctgccttcgc ggcacgccgc gccagcgtgt tcttcacaac catgaagccg atgttttccg
     7081 ccgcgcaacg cttgcggagg ttcgtgacaa gttccacctt caaacccgtg gaatccgtca
     7141 ggaatatccc gttcgccgaa ttgaacatgt cggtcaaacg ggctatcgtc tcattctttt
     7201 tcggggtcgg catcgcctga accttcttca acgaattact tcacgagcga ctggaactgg
     7261 ttcctgtcca gcggcacact gggcgtcatg ctggcacaca gattggcgga gtgaacgtac
     7321 tggcccttca ccgccgtcgg ccgcgcacgc atcactgcct caaagaacac ctgcgcgttc
     7381 gcgtacaacg cgtccacgct gaaggataac cgtcccagcg gcgcctgcac gtttcctgcc
     7441 ttgtccacgc ggaaatctat cttgccgcct ttggcggcct gcacggctgc tgccgtatcc
     7501 tgtgtcacgg tgcccgtctt cgggttcggc ataaggccac ggggaccaag cacgcgcccc
     7561 agacggccca ccacacccat cagatccggg gtggcaatcg ccacatcgaa atccgtccac
     7621 ccctcctgaa tcttcgccac caggtcatct gcacccacga aatccgcccc tgcctgttca
     7681 gcggccgtcg cggcctctcc gcgtgcaaac accagaacgc gcaccgtttt ccccgttccg
     7741 ctgggaagaa cgaccgatcc acgaacgagc tggtcggcct tccgcggatc gacattcaac
     7801 ttcatcacgc cttcgaccgt ctggtcgaaa ccgcgcggct caaactgctt gattacctcg
     7861 attgcttccc gcaacccgta actcttggtg cgatccacct tttcgaggtc atgctgatat
     7921 ttcttcgcca gtttcggcat ggttatgtcc tctacttggt gggtcttcca acaacttcca
     7981 gccccatgct gcgcgcagtg ccggcaatca tgctggcggc ggaatcaagg tttgtggtgt
     8041 tgagatcagg aagcttggtc ttcgcgattt ctaccacctg atcccacgtg accttgccca
     8101 ccttcttcgc gttgggctcg ggtgaacctt tttccagccc ggcggccttt ttcagcaaag
     8161 tcaccgcggg tggcgttttg aggatgaacg tgaaactgga atccgcgtag gcggtgataa
     8221 ctgcaggaat gatcatcccc tgctgggacc gcgtccgttc gttgaacgct ttcacgaact
     8281 ccatgatgtt gagccgatgc tgcgaaagcg ccggccccac cggcggcgca ggagtagcct
     8341 ggccggccgg caactgcaac ttgatctgcg cgatgacttt ttttgccacg tgctacctcc
     8401 gatgccctcc acctcacaac cgaggtcagg ctatcgttat gttgaagtgc gccgcctccc
     8461 ggagaacacc gggaaacagc ctgatcatgc tttcgggggt gctggttcga tctgcagaag
     8521 gtccagctcc actttcgtcg ggcggccgaa gatgctcacc gcaatcgtca ctttgccctt
     8581 cgccgtgtcg aacgcttcca ccacgccaac gaaatcgctg aacgggccat ccacgacttt
     8641 cacaaaatcg ccgggacggt acgcagagtc cgtctccggc gccgtgcttt tccgctcaac
     8701 ctgctgaaga atgcgctcca catcgctcgg acggagaggc gcgggttcct tgctcgaacg
     8761 accacccgtc gtaacaaaac ccgtaacccc tggggtgttc tccaccacgt accgcgattg
     8821 gctggtaagc tccatctcga tgaggacata cccgggcagg aaacgacgct tcgtcgtaac
     8881 gcgtttgccc tcgcgcatct ccggcacatc ctcaatcggg atcacgactt cccctatctg
     8941 atcgcccagc gcgagagcct ccgccgagtt cttcagatag tgaagcacct tgttctcatg
     9001 ccccgaaagc gtatgcaccg cgtaccagcg cttcgggcgc ggagcttcac cctgtggtgt
     9061 atcagtcata acctaccgca aaaaccattg aacgatctgg ctcaaaatcc agtcaaaggc
     9121 accgatgaat acggcaaaga tggccgtgac tatcagcacg acgatagtcg agttcttcaa
     9181 ttggtcacgc gtgggccacg ataccttgcc catctccatg actacttccc ggtaatatgt
     9241 cgccactttc tgaaacacgt cgcctgtccc ggccagttaa cggagaatgg caggagtgga
     9301 gggattcgaa cccccgaccg tcggatttgg agtccgatgc tctagccaga ctgagctaca
     9361 ctcctacccg tatctcgcag tgtgagtgat tatcgtgtct cacggtgcgg cgtgtgcttc
     9421 ttgcaccatg ggcagtattt cttgtaggtc acacgttcag aatgcttccg cttgttcttg
     9481 tcgcccgaat agttgcggcg tttgcagtct tcacatgcca gagtgatgat atcgcgtggc
     9541 ataggcccgc gcacctttca atcaaaagaa gtatcgttcg ctgcgtccct gcttactgca
     9601 gaatttttgt aacggagcct gcgccgacgg ttctgccgcc ttcacg
//
LOCUS       DAOTNG010000094         9619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6528_length_9619_cov_4.559389, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000094 DAOTNG010000000
VERSION     DAOTNG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_9619_cov_4.559389"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="P5179_06080"
     CDS             complement(<1..1000)
                     /locus_tag="P5179_06080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRS94821.1"
                     /translation="MAVDTMEQIVSLCKRKGFIFQSSEIYGGLNGCWDYGPLGVELKR
                     NVQDAWWRAMTVEEDDIEGLDASILMHPRVWEASGHVAEFTDPLVDCTHCKARFRDDQ
                     IEKEGQCPACGMKGTLTAPRKFNLMLKTFIGPVEEAASVVYLRPETCQGIYVDYQNIM
                     TAMRRRIPFGIAQIGKAFRNEINPRYFVFRSREFEQMEMQYFVKPGMDMDVFNAWRDK
                     RLRYYAELGIRPEKLRLVPHEKLAHYASAAVDIEYEFPFGWGEIEGIHNRTDFDLRRH
                     SEFSGKDLSFFDDQTRERYIPFIIEASSGVNRTLLAYLCDAYEVEQLENDTRTVLHL"
     gene            complement(1064..1843)
                     /gene="recO"
                     /locus_tag="P5179_06085"
     CDS             complement(1064..1843)
                     /gene="recO"
                     /locus_tag="P5179_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRS94822.1"
                     /translation="MPGVDFTRALVISARPWSESSRIVTILSPDLGKFRAVCRGVQKA
                     QGGMAAALEPITLSEIVLRQSGRGGMAHISAADAVEYFACTKKSLIGLAAAGGLLELC
                     EAAMPENEPGDAVFDHLLTLLNELEGLLRKREHSERNAINLLWRGILVLSGDFGYGMQ
                     FENCVACGALRPRAKGFCLAEGGPVCDACSVPNTLNWGPGTEETLRWLAVAEDSALPE
                     RTIPRTQNREIRTLFERYFRFHVPGFDHLRSLDMLVTERDQ"
     gene            complement(1861..2541)
                     /locus_tag="P5179_06090"
     CDS             complement(1861..2541)
                     /locus_tag="P5179_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRS94823.1"
                     /translation="MMPNGPSTGWRHWAKANFLTGLVALAPVVITGYVLWYVFATVDA
                     WLSAALVGIPWLTWRGKPLPGVGFVTVLLIILLTGVVARNVAGFQFLRFWDRQLNRIP
                     MVRGIYRAVKQISQAFFGGTRSVFQAVVLVPFPTRGIYAVGFLTSDAPREVCNKTGQD
                     LVSVFLPTSPNPTSGYLIMVPRAEVTRVEMTVEEAMKLVISGGTVLPPEAESGADGVT
                     ERAQSNAG"
     gene            complement(2538..2918)
                     /locus_tag="P5179_06095"
     CDS             complement(2538..2918)
                     /locus_tag="P5179_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRS94824.1"
                     /translation="MTEAAGDQNPLRTWKEQQRRRRSFARRWKILTLAAAVVVVVAAL
                     FWGGGDGFFVQWEVSREIAALEKENALIRVQNAQLRDHIRRLDSDMSYIERIARERYG
                     MALPHERVYRVFIPPAPTAGSAGP"
     gene            complement(2915..4171)
                     /locus_tag="P5179_06100"
     CDS             complement(2915..4171)
                     /locus_tag="P5179_06100"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRS94825.1"
                     /translation="MDLGAYINELGTKARKAAIEMARADSATKRDALLRIAEAVEKHS
                     GRILESNARDLAAGKAAGLTQAMLDRLTLTEKRIVAMAHAIAEIADQPDPVGEIEHSV
                     IRPSGIRVGRMRVPIGVIGIIYEARPNVTTDAAALCLKAGNACVLRGGKEALHSNVAL
                     ARVVSETAAAAGLPPDAVQLVSVTDRETVSLMAQAEGLIDVIIPRGGEALIRSVVAAA
                     KVPVIKHYKGVCHTFVDADADLDMARRICFNAKVQRPGVCNAMETLLVHEKVAEAFLP
                     TLFAEYREAGVSLRGCNRTIEIDPTVARATEEDWYAEYLDLILAVRVVSSLDEAIEHI
                     RRYGSAHTDAIVTRSIQTAERFVREVDSSSVMVNVSTRFSDGGEYGLGAEIGISTDKL
                     HARGPMGAADLTTTKWVVTGNGAIRQ"
     gene            complement(4195..5394)
                     /locus_tag="P5179_06105"
     CDS             complement(4195..5394)
                     /locus_tag="P5179_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS94826.1"
                     /translation="MGRNTAYLEAAREIIPWGTQTNAKRHHAEMGEAMPAFIERANGC
                     RMWDLEGREYIDYRCALGPIILGYRYPAVDEAVRIQMSKGTLFSMASPVELEVAKEIV
                     ELVPCAEMVRFMKTGADANMCNVRIARAYTGRDHIIICGYHGYHDYFMVTQQGNGVPQ
                     AVRDLVTPIGYGDFEKLRETFRTRGETIAAMVMEPFNAKDPAGEFTKEARKLTSEYGA
                     LLIFDEIITGFRLAKGGAQEYLGITPDLASLGKAVANGYPLSVYCGKRQFMSKLDDFV
                     LTTTYAGDTLSLAAARATLAVMQNEPVHKHLYRVGERLQKGIGEVAGKVGVEMAITGN
                     PVQPGFAIVDGDKEKESALNTEWRRQLFAHGVFPHWFVNYSHQPSDIDETIDKAEKAL
                     RATLKRG"
     gene            complement(5527..6954)
                     /locus_tag="P5179_06110"
     CDS             complement(5527..6954)
                     /locus_tag="P5179_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94827.1"
                     /translation="MTSLEGVATHATGLPVRNRDRVDHHTQAEECWMVFPQSLKGIAA
                     LFLIAKCGVALTPKMQTIKDISVGVGEQLPSGWVGLGARSQAMGGTLAASTIDGTALF
                     VNPAQLTSVRRTEILGSLAMSRIRGTADTGNPVTTSELSATGTALNSLVVSAPYPVYR
                     GGFTFALGYARPSDYGQRMNRTGIVTLQGQVFRQSDILRQEGGLSHYAMGFGVEVAPT
                     TSMGLSLRWQHGSVDVRRDITLVAQGLALPDSLVGRYRQHNSIDGLGVVLGTHTVLPA
                     GFQMGLSVSTPTRYTLDGTWGDEYHEAIGANVYHYDYLENKLKYKIRSPWEFGAGLTW
                     TTYALTLAADVWYADWQQARFDGSPYGASSGIDPDTFFETRYRGSLRWHIGGEFLVPV
                     VNTYVRAGYWTGTEPFRGPLLDSGEALTVAGPSKAYSFGAGWLIDRVFALDAAVVFRT
                     GRMGTAQSVEKTGDRRFSVSAAFRM"
     gene            complement(6893..7663)
                     /locus_tag="P5179_06115"
     CDS             complement(6893..7663)
                     /locus_tag="P5179_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94828.1"
                     /translation="MNRTPRGRRSLAGLLILGSLSLYGCYTVLSHPQLAPRQQITENG
                     TVVETDQCASCHTQSDLWSFHHRGWYSQYNSYFYNQYDPLFFRSRWYGDSYFYGRWVS
                     YRYSPWWYYPGSRTGAWHPELPAVGRGQVRDSVNSVDPRDSFVGLPGYGPGPMPSFPR
                     SALPLNTGMAGSPATSRVTRDSTSADRGSATEGRPVRVEPRNDAPAPLPVSQQPVQQP
                     AATKSADKPQESEDQEQAGDQPRGRGDARNRPSGSQSR"
     gene            complement(7808..>9619)
                     /locus_tag="P5179_06120"
     CDS             complement(7808..>9619)
                     /locus_tag="P5179_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRS94829.1"
                     /translation="ERVVAWRNGRPVIPVRAHRRNTVRGIVHDESASGQTVFVEPFES
                     VEDANRIRRLEIAEQAEVDRILSELTGVVFQHSPRLRVNLQILGRLDALYALGVYADQ
                     ADAAVPGISDDGALEIVRMRHPLLDVRLRGQKREAVPLTCRLRPDDRLMLISGPNAGG
                     KTVALKTIGLAIAMTQSGFPIPADPLTTLPVFESFFAEIGDEQSIENDLSTFSSRIGH
                     LARISAEAGKGTLILVDELGSSTDPDQGAALSRALLERWFEKGARTVATTHLGSLKEF
                     AHEMAWAVNASMEFDRDKLNPTFRLVAGIPGSSYAFEISRRIGLAEDIIARAEHFLGR
                     AAVQTERLIADLTFLMEQTERRLREIESRERALAEREREYEDRFARVVSDRKRMAREA
                     REEAEKILADARALVERTVADIRRTNGDRESIKSARATLQSALARTRSELADRGRTDG
                     AEATGINPGNWVKIVSINKTGEVVAVSKDRVAVQTGGTRLEVRPDQVEKIAEKAPDAG
                     KGGVSVTTTASDRFLPEVDVRGMSFDDAWAVLDRYLDDACVARYPRVRIIHGKGTGVL
                     RRRINEQLKQDERVASHQMGEYYEGGAGVTVVDMKLD"
BASE COUNT         1909 a         3042 c         2624 g         2044 t
ORIGIN      
        1 gcaggtggag caccgtgcgt gtgtcgtttt ccagctgttc aacttcgtag gcatcgcaca
       61 ggtacgcgag cagtgtgcgg tttacccctg aagatgcctc gatgatgaat gggatatatc
      121 gctcgcgcgt ctggtcgtcg aaaaagctca ggtccttgcc ggagaactcg gaatgacgcc
      181 gcagatcgaa gtccgttcgg ttgtggatac cttctatctc accccatccg aaggggaact
      241 catactcgat gtccacggca gcggaggcgt aatgcgccag tttttcatgc ggcacgaggc
      301 gcagtttttc cggtcgtatg cccagttccg cgtagtaacg gagtcgtttg tctctccacg
      361 cattgaacac gtccatgtcc atacccggct tcacaaagta ctgcatctcc atctgctcga
      421 actcgcggga ccgaaacacg aagtagcgcg ggttaatctc gtttctgaac gccttcccga
      481 tctgagcgat cccgaacggt atccgccttc tcatcgccgt catgatgttc tggtagtcaa
      541 cgtagatccc ctgacacgtt tccgggcgca gatacacgac ggacgccgct tcttcaacgg
      601 gtccgatgaa ggttttcagc atcaggttga atttgcgcgg agccgtcaga gtgcctttca
      661 tgccgcaggc cgggcactgg ccttctttct cgatctggtc atcccggaag cgcgccttgc
      721 agtgagtgca gtccaccaga gggtcggtga actccgccac gtgccctgaa gcttcccaga
      781 cgcgagggtg catgagaatg ctggcgtcga gaccttcgat atcgtcctct tccactgtca
      841 tggcgcgcca ccatgcatcc tgaacgttcc gcttcaactc gacgccaagg ggaccatagt
      901 cccagcagcc gttcagtcca ccgtatatct cgctggattg aaaaatgaac cccttcctct
      961 tgcacagtga gacgatctgc tccatcgtgt ccacagccat gaaagctcct tcgttgacct
     1021 gtgtcgttga cgacccgttc gcaataacgt cggctgaatc ggcctattgg tcgcgttcag
     1081 taaccagcat atcaagtgac cgcaaatgat caaatcccgg gacatgaaac cggaaatacc
     1141 gttcaaagag ggtgcgaatt tctctgtttt gcgtccgcgg aatggtgcgt tccggcaatg
     1201 cgctgtcctc ggcaactgca agccagcgga gggtttcctc cgttcccggt ccccagttca
     1261 aggtattcgg aactgagcat gcgtcgcaca cgggacctcc ttcggccaga cagaacccct
     1321 ttgcacgagg ccgaagcgcg ccgcacgcca cgcaattttc aaactgcata ccgtatccga
     1381 agtcgccaga aagcacgagg attccgcgcc aaagcaaatt gatcgcattc cgctcgctgt
     1441 gttcccgctt ccgaagcaat ccctccaact cgttgagcag ggtaagcagg tgatcgaaca
     1501 cggcatcgcc cggttcgttt tccggcatgg ccgcttcaca caactcaagc agcccaccag
     1561 ccgctgcaag gccgatcagg gatttctttg tgcaggcgaa gtactccacc gcatccgcag
     1621 cggaaatatg ggccatgcca ccacggccac tctgccgcag gactatctcg ctcagtgtga
     1681 tcggttcgag cgccgccgcc attccaccct gagccttctg aacgccgcgg cagactgcac
     1741 ggaacttgcc cagatccgga gaaagaatgg ttacgatgcg actggattct gaccagggcc
     1801 gtgcggagat caccaacgcc ctcgtgaagt caacgccggg cattttcagt ctctcccgcc
     1861 tcaacccgcg ttcgactgcg cgcgctcggt taccccatcc gctccggact ccgcttccgg
     1921 tggcagcact gttccgccgg aaatcaccag cttcatggct tcttccacgg tcatttcaac
     1981 gcgtgtaacc tcggcgcgcg gaaccattat caggtagccc gacgtagggt tcggcgacgt
     2041 ggggagaaaa acgctcacaa ggtcttgacc cgttttgtta cacacttctc tcggcgcatc
     2101 agaggtcagg aagcccaccg cgtagatgcc gcgtgtggga aatggcacga gtacgaccgc
     2161 ctgaaacacg ctgcgggtcc caccgaaaaa tgcctggctg atctgcttga ccgcgcggta
     2221 gatgcctcgc accatcggga ttctgttgag ctggcgatcc cagaaacgga ggaactgaaa
     2281 tcccgccacg ttcctcgcga ccacgccggt cagcagtatg atcagcagta cggtgacgaa
     2341 ccccaccccg gggagcggct ttcctcgcca cgtaagccat ggaataccga caagcgcggc
     2401 cgagagccat gcatcaaccg tggcgaagac ataccagagg acgtacccgg taatcactac
     2461 cggcgccagt gcgaccaatc cggtgaggaa attggctttc gcccagtgcc gccatccggt
     2521 cgagggcccg ttcggcatca tggacccgcg cttccggccg ttggggctgg agggataaaa
     2581 accctgtaca cacgctcatg cggaagtgcc attccgtacc gttcgcgtgc gatccgctct
     2641 atgtatgaca tgtcgctgtc gaggcgacgg atgtgatccc gcaattgagc gttctgaaca
     2701 cgtatcaacg cgttttcctt ttccagagcc gcaatttccc ttgacacttc ccattgaaca
     2761 aaaaaaccat cgcctccgcc ccagaacagc gctgccacca ccacaacaac tgctgctgca
     2821 agggtgagaa tcttccacct gcgtgcgaat gaacgtctcc gacgctgttg ctctttccag
     2881 gtacgaagag ggttctggtc gcccgccgct tcagtcactg ccgaatggct ccgttaccgg
     2941 ttacgaccca tttcgtggtg gtcaaatccg ctgctcccat cgggccccgg gcgtgcagtt
     3001 tgtctgtaga tatgccgatt tccgctccca gtccgtattc gccaccatcg gagaaacgcg
     3061 tggaaacgtt gaccatgaca cttgacgagt ccacctcgcg cacaaaacgt tcggcggtct
     3121 ggatactgcg tgtcacaatc gcgtccgtgt gcgcggaacc gtacctccgg atgtgctcga
     3181 ttgcctcgtc gaggctgctt accacccgga cggcaaggat gaggtcgagg tactcggcat
     3241 accagtcttc ttccgtcgct ctggcaacag tgggatcgat ttcgatggtt ctgttgcacc
     3301 cgcgcagtga aactccggcc tcacgatact ctgcgaaaag cgtcggaaga aaagcttcgg
     3361 cgactttttc gtgcacgagg agggtttcca tggcgttgca tacgcccggg cgctggactt
     3421 tcgcgttgaa acagatcctc cgcgccatgt ccaggtctgc gtcggcatcc acgaacgtgt
     3481 ggcacactcc cttgtaatgc ttgatcaccg gaaccttcgc cgcagcaacc accgaacgga
     3541 tcagagcctc gcccccgcgg ggaatgatga cgtcaatcag cccttcggcc tgcgccatca
     3601 agctcacggt ttctctatcg gtaacgctaa caagctgcac ggcgtccgga ggcagacccg
     3661 cggccgcggc agtctcggat acgaccctgg ccagagccac attggaatgg agagcttcct
     3721 tgccaccgcg caacacgcag gcgttgccgg ccttcagaca aagcgctgcg gcgtcggtcg
     3781 tcacgttggg acgtgcttcg tagatgatac cgattacccc gattggcacg cgcatcctcc
     3841 cgacgcgaat gccgctcggt ctgatgacac tatgctctat ctcgcccacg ggatcgggtt
     3901 gatcggcaat ttcggcgatc gcgtgcgcca ttgctacgat acgcttctcg gtcaacgtaa
     3961 ggcggtccag catggcctgg gtcagaccgg cggctttgcc cgccgccaga tcacgggcat
     4021 tcgattcgag gattcgcccg gaatgcttct ccaccgcttc cgctatgcgc agaagtgcat
     4081 ctcgtttggt cgcggaatcc gcgcgcgcca tttcaatcgc ggctttgcgc gctttcgttc
     4141 cgagttcgtt tatgtacgca ccaaggtcca tcatgtgccc caatgtccag tgccctaccc
     4201 acgtttcaaa gttgcccgga gagccttttc ggctttgtca atcgtttcat caatgtcgga
     4261 cggttgatgg ctgtagttca caaaccagtg gggaaatacg ccgtgggcaa aaagctgccg
     4321 cctccactcc gtgttcaggg cggattcctt ttccttgtct ccgtccacga tggcaaaccc
     4381 gggttggaca gggttccccg tgatcgccat ttcgacgccg acctttccgg ctacctcccc
     4441 aatccctttc tggagcctct ctcccacgcg atagaggtgt ttgtgcactg gttcgttctg
     4501 catcactgcg agcgtagccc gggccgccgc cagggaaagc gtatcgcctg cgtaggtcgt
     4561 cgtgagaacg aaatcatcga gtttcgacat gaactggcgt tttccgcagt acacagacag
     4621 cggatatccg tttgcaacag ccttcccaag agaggcgagg tccggtgtga ttcccagata
     4681 ttcctgcgct ccgcccttcg cgaggcggaa cccggtaatt atctcgtcaa aaatcaacag
     4741 cgccccgtac tcggatgtca gttttcgggc ttctttcgtg aactcgcccg ccgggtcttt
     4801 cgcgttgaaa ggttccatca ccatggcggc aatcgtttcg cccctggttc ggaaggtttc
     4861 gcggagtttc tcaaagtccc cgtaaccgat tggagtcaca aggtcacgga cggcctgtgg
     4921 aactccattt ccctgttgag tgaccatgaa atagtcgtgg tatccgtggt acccgcagat
     4981 tattatgtga tctcgaccgg tgtacgcacg ggctattctc acattgcaca tgtttgcgtc
     5041 agcaccggtt ttcatgaagc gaaccatctc cgcgcaggga accagttcga ctatttcctt
     5101 cgccacttcg agttccaccg gcgacgccat gctgaaaagt gtgcccttgg acatctggat
     5161 gcgtaccgct tcgtctactg ccgggtaccg gtaccccaga atgatcggtc cgagggcaca
     5221 gcggtagtct atgtactcgc gtccctcaag atcccacatc cggcacccgt tggcacgttc
     5281 gatgaaagcc ggcatcgctt cgcccatttc ggcgtgatga cgtttcgcgt ttgtctgcgt
     5341 gccccaggga atgatttcgc gcgcggcttc caggtaagcg gtattgcgcc ccattgttca
     5401 acctccctca aatggtaccc ggaaacgaac cagatctccc ataaaatgaa cacgcaacga
     5461 aggtttttga gcaaccctga agaagctctt tgcggaaaac cgtgcgtcgc ccgtcccccg
     5521 cagggatcac atgcggaacg cggccgaaac tgagaatctc cggtctccag ttttttctac
     5581 cgattgagcg gttcccatgc gaccggtccg aaacactacg gccgcgtcaa gcgcgaacac
     5641 tcgatcaatc agccaccccg cgccgaacga gtaagccttt gaagggcccg ctacggtcag
     5701 cgcctccccc gaatcaagca gcgggccccg gaacggttcc gtgcctgtcc agtaccccgc
     5761 gcgcacatac gtgttcacga ctgggacgag gaactcgcca ccaatgtgcc accgcaacga
     5821 gcctcggtaa cgcgtttcaa aaaacgtatc agggtcaatc cccgaagacg caccatacgg
     5881 gcttccgtca aagcgggcct gttgccagtc cgcgtaccag acgtcggcgg caagggtcaa
     5941 cgcataggtg gtccacgtca gtcctgctcc gaattcccag gggcttctga ttttgtactt
     6001 cagcttgttc tcgagatagt cgtagtggta tacattcgcc ccaatcgcct cgtgatattc
     6061 gtcgccccac gtaccgtcca gagtataacg cgttggcgtc gacacactca gccccatctg
     6121 gaaacccgcc gggagtaccg tgtgggttcc caagacgacc cccagaccgt caatcgaatt
     6181 gtgctggcgg taccgcccta cgagcgagtc tggcagggcc agtccctgag ccaccagtgt
     6241 gatatcgcga cggacatcca ccgatccgtg ctgccagcgc aggctcaaac ccattgacgt
     6301 tgtcggggcg acttccacgc cgaagcccat ggcataatgg gacagccctc cttcctgtcg
     6361 cagaatgtcg gattgccgga atacctgccc ctgcagcgta actatccccg tgcggttcat
     6421 tcgctggccg tagtccgacg gcctcgcata tccgagcgcg aacgtgaagc cgccgcggta
     6481 caccgggtac ggagcgctta cgaccagcga attcagcgcg gtgcctgtcg ccgacagctc
     6541 gctcgtcgtt acgggattgc ccgtatccgc cgtaccacga atacggctca ttgccagaga
     6601 accaagtatt tccgtacgcc gcactgaggt cagttgcgcg gggttaacga aaagcgcggt
     6661 accgtcgatg gtgcttgccg caagagtacc ccccatcgcc tgcgagcgcg cacccagtcc
     6721 tacccatccc gaaggcaact gctcccccac gcccaccgag atatctttga tagtctgcat
     6781 tttcggggtg agggcaacac cgcacttcgc gatcaagaaa agcgcggcga tgcctttcag
     6841 gctttgcggg aacaccatcc agcactcctc tgcctgtgta tgatgatcaa ctctatcgcg
     6901 attgcgaacc ggaaggcctg ttgcgtgcgt cgccacgccc tcgaggctgg tcacccgcct
     6961 gctcctgatc ctcggattcc tggggcttgt ccgccgattt ggtggcagca ggctgttgaa
     7021 ccggttgctg gctgaccggg agtggagccg gagcgtcgtt ccgtggctca actcggaccg
     7081 ggcgcccttc ggttgcggaa ccgcggtctg cggaagtaga atcacgtgtg acgcgcgagg
     7141 ttgccggaga acctgccatt cctgtgttca acggcagcgc cgaccgcgga aatgaaggca
     7201 tcggaccggg tccgtacccc ggcaatccaa cgaaactatc gcgcggatct acactattga
     7261 cgctgtcgcg cacctgacca cgtccaacag ccgggagttc gggatgccag gcacccgtcc
     7321 ggcttccggg atagtaccac cacgggctgt agcggtagct gacccagcgt ccgtagaagt
     7381 aagaatcacc ataccaccgt gaacggaaga acaacgggtc atactgattg taaaaatagg
     7441 agttgtactg cgagtaccat ccgcggtgat gaaacgacca caagtcactc tgggtatgac
     7501 acgaagcgca ctgatccgtc tccacgacgg ttccgttttc cgtaatctgt tgccgcggcg
     7561 caagttgagg atgcgagaga acggtgtaac agccgtacag gctcaaggac cccagtatga
     7621 gcaagccggc cagactacgt ctgccccgcg gcgtcctgtt cacagtggaa cctccttgat
     7681 cgaagatcca atcggtacgc gggcctctgg cggattcgcc atatgctcat ccataagata
     7741 ctctgccccg acgcgaaatg tccgcgccgt tcgtctgagg ttctccagta tttggaagcc
     7801 ccagctgtca gtccagtttc atatccacta ctgtcacacc ggcgccccct tcgtagtatt
     7861 cgcccatctg atgggaggct acccgttcgt cctgtttcag ttgttcgttg atcctgcgcc
     7921 ggagcacacc ggtgcctttt ccatgaatga ttcgaacgcg agggtaccgc gcgacgcacg
     7981 cgtcatcgag ataccgatcc agaactgccc acgcatcgtc gaaactcatt ccccggacat
     8041 ccacttcagg aaggaaacga tccgacgctg tggtagtaac ggagacgccg cctttgcccg
     8101 catccggtgc cttctccgct atcttttcca cctgatccgg tcttacctcc agacgcgttc
     8161 cgcctgtctg aaccgccaca cgatccttgc tgacagccac cacctcgcct gtcttgttga
     8221 ttgaaacgat cttcacccag tttccggggt ttattcccgt cgcctcggct ccgtcagtgc
     8281 gaccgcgatc agcaagttcg gacctcgtcc gcgcaagcgc gctctggagc gttgcccgcg
     8341 cgcttttgat ggactccctg tcgccattgg tacgccggat atcggcgacc gtgcgttcca
     8401 caagggcgcg cgcgtccgcg aggatctttt ctgcttcttc gcgtgcttcc cgcgccatgc
     8461 gtttccgatc tgaaaccacg cgtgcgaacc tgtcctcgta ctcccgttca cgttcagcga
     8521 gcgcgcgttc tctggactcg atctcgcgca gtctgcgctc cgtttgttcc atcagaaagg
     8581 tcaggtcggc gataagtcgt tccgtctgca ctgccgcccg accaaggaag tgctccgcgc
     8641 gggcgatgat gtcttccgcc aagcctatcc ggcgactgat ttcaaaggca taactggaac
     8701 caggaatgcc cgccaccaaa cgaaaggtcg ggttcaattt gtccctgtcg aattccatcg
     8761 acgcgttgac cgcccaggcc atttcgtgag cgaattcctt gaggcttcca aggtgtgtcg
     8821 ttgcgacggt acgagcgcct ttctcgaacc agcgttcgag cagcgcgcgg ctcagcgccg
     8881 caccctggtc ggggtcggtt gacgaaccaa gttcatccac caggatcagt gtgcccttgc
     8941 ctgcctctgc ggaaatgcgg gcgaggtgcc cgatgcgcga actgaaagtg gaaagatcgt
     9001 tctcaatcga ctgttcgtcg ccgatttccg caaaaaagga ttcgaagaca ggaagtgtcg
     9061 tgagcgggtc cgcgggaatc ggaaaaccag actgcgtcat cgctatcgca aggccgatgg
     9121 tcttgagagc gaccgttttt ccccccgcgt tcgggccgct tatcagcatc aaccgatcgt
     9181 caggccgcag ccggcaggtg aggggtaccg cctctctttt ttggccgcgc agacggacgt
     9241 ccagcaaagg gtgtcgcatc cggacgatct ccagtgcgcc gtcgtcagat atcccgggca
     9301 cggcagcgtc agcctgatcc gcgtacacac cgagcgcgta gagtgcgtcc aaccgtccga
     9361 ggatctggag gtttacccgg agtcgaggcg aatgctggaa cacaaccccg gtcagttctg
     9421 aaagaatccg atcgacctcc gcctgctcgg cgatttccag ccttctgatg cggtttgcgt
     9481 cctcaaccga ctcgaacggt tccacaaaaa cggtctggcc ggacgccgat tcgtcgtgga
     9541 cgatgccgcg caccgtattg cggcggtgcg cccggacagg aatgaccggc ctgccattcc
     9601 gccatgcgac aactctctc
//
LOCUS       DAOTNG010000095         9597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6549_length_9597_cov_5.154789, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000095 DAOTNG010000000
VERSION     DAOTNG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_9597_cov_5.154789"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="P5179_06125"
     CDS             complement(<1..601)
                     /locus_tag="P5179_06125"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRS94830.1"
                     /translation="MKKWIRTIILVVIALALGFWAYFGERHLDKIALEKRLEQKITRI
                     PTEKIAGFTLIINNPNAPKPDTTVVERVGSDEWQIVHPILWPGESTEWRALAQNLPRA
                     WKQRAWKSSPDSVAFFGLKPPLVRLIIKPVDSAMRPDTLDIGKATPTDNFCYVKYPRS
                     DSIYTTDVILVSSTLKSLHTLRDKSVTPIEPSLVQRVTVK"
     gene            complement(716..2476)
                     /locus_tag="P5179_06130"
     CDS             complement(716..2476)
                     /locus_tag="P5179_06130"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRS94831.1"
                     /translation="MVRSIPIPFIALVGLLLAVDGMVAWLASATVWGWGIPWAVGTGL
                     LLGAFLSRLGIRKSVAFAAALVVLIALCAIIYVKGEVIGAFKLDSITAILLFLGLFVG
                     EVGVGIASVLTGRASAQGANFWVYVVSALAIAVVLNYLTNRHVRTTYDITQAQLESLS
                     DQTLAKMKGLRKDVHLIAFIRDSDPNRPGYAEMLKKYAAASRYFTFEFVDPDKYPDIA
                     ARENINPNDITIIVKCEDRRELVLGPEERDLTTAIIKVTRPGQKRIYFSMWHQERTLE
                     GEMGMLANLLRQLNYATAPLKLIDSDVPPDCAVLVIPGPKAPFAPVEISRIERYLAEG
                     GSALVLLDPDSIETGLENVVARWGVVVHPNMVIERQRGLVPYAGGLYMGEQQSMYARA
                     MKYEPSHAIVADLESKGIATGFYQVREITWTGANPTLACTGTPIVFAGSRTSFAITEV
                     KEALRNPRQAHSAEGKPSGPFSVAVAATAPPSVSTGVEGAKTRLVVVGDADFATDRVV
                     SQERGNVDFMLNCITWLSEDEDLITIHSREPGYKPIVMTASQGTFMFLFCVWRYPAMV
                     FVAGLLIWWYRRSRGPGKDN"
     gene            complement(2477..3286)
                     /locus_tag="P5179_06135"
     CDS             complement(2477..3286)
                     /locus_tag="P5179_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS94832.1"
                     /translation="MKGTWTVFVKELRSYVHSPVAYVMAFVFLILCGYFFQDAMNFLN
                     RYTTDYPMRYQQAQQMRRDPPPPPNVDIVVIRRTYSVMAFLLTFIMPILTMRLYSEEY
                     RNGTIELLFTSPVTPGQTLAGKFLASFALYAGILVLTLNFVVFAYLYAEEGVRPDTGL
                     ILSCYLGLLFLGASFISVGVFASALTENQIVAAVVSFSVLLMFLVIGAAADYVGNYSA
                     AEFLRYLAVNGHADSFLRGVISLRDIVYYITFIGFMLLLTNRVLESRRWRL"
     gene            complement(3367..4017)
                     /locus_tag="P5179_06140"
                     /pseudo
     CDS             complement(3367..4017)
                     /locus_tag="P5179_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4113..5018)
                     /locus_tag="P5179_06145"
     CDS             complement(4113..5018)
                     /locus_tag="P5179_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94833.1"
                     /translation="MRYIRVLLVIGLIAFGTGCSKSGVDPEKQRVFATELLNRELYAE
                     AIRAFDKYLEMPAVSDRDRVDAMRKLADALFDRANDYHSALVYYLRLRVFAPDYPEMN
                     EVRARLVTCFERTGRSADASLMRREIAQGKTLPPDSLAGPVVAEFGGRKISEREVLRE
                     LEQLPPELRQQFSTMERKRELLRQVVGREILYEMAVKRGYADSPEFQLQLDRIRRDML
                     VQRIGEEQLGSLPDITEADVRRFYEEHQAELPRVPGGGVPSLQQIRPQIEMAARQAKQ
                     QEAFQRLVDQLFASQEVKLYPERMR"
     gene            complement(5127..6224)
                     /locus_tag="P5179_06150"
     CDS             complement(5127..6224)
                     /locus_tag="P5179_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS94834.1"
                     /translation="MIQKEILKKVRRIEIQTRQVVNTLLSGEYHSALKGQGMEFAEVR
                     EYQPGDDVRRIDWNVTARMRRPFVKIFDEERELTVMLMVDASSSGDFGSVEQTKNQIA
                     VELCALLAFSATKNNDRVGLIVFTDRVEKFVPPKKGRKHVLRVIRELLAQFSRTETSE
                     PRPFRSRPHPVMGGLASLAILAAAASMISAIGIPETTYQWLAETVISILGAAGVGVVS
                     WWAFRTYGADKGRGTDIAGALDFLNRVMTRRGVVFLISDFQAAGFEKALKAARRKQDV
                     IAITLTDPREVTLKGAGLLALEDPETGESVVVDSSGKRFRDWYEQQARTRHAQRKRLF
                     RTTGVDCVDVWTDTSYVDPLVRFFSARAKRM"
     gene            complement(6221..7216)
                     /locus_tag="P5179_06155"
     CDS             complement(6221..7216)
                     /locus_tag="P5179_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRS94835.1"
                     /translation="MPLDSHIQEIHAEIERESEFVERIVSEIGKVIVGQRTMVERMLI
                     GLLCDGHILLEGVPGLAKTLAVSSLARTIHAKFQRIQFTPDLLPADVVGTPVYEQKKS
                     TFTVKKGPIFANIVLADEVNRAPAKVQSAMLEAMQEHQVTIGDQTFALEDPFLVLATQ
                     NPIEQEGTYPLPEAQIDRFMLKVVVTYPSKAEERQILDRMTEGDVPHVNEVVEPADII
                     RARQLVRRIYMDEKIKNYIVEIVCATRKPAEYGLADYTDFVDYGASPRATIWLALASK
                     AHAFLRHRGYVTPEDVKAIGMDVLRHRVIPTYEAEAEEVTAEAIVSKVFDSVEVP"
     gene            complement(7206..8192)
                     /locus_tag="P5179_06160"
     CDS             complement(7206..8192)
                     /locus_tag="P5179_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRS94836.1"
                     /translation="MGSELHLGLINGTRVDPAQVSYTGVVPHRGILHVPLLSLCVVLL
                     LAVGDAWPADDIRIEAQLDRGTVALDETATLVVKAEWEGPSDRFALVIPQAPRVHNLI
                     VIGSKRGTRSWADEKGEHSAQEFSYVLQPESEGNAAVGAIRVQFTTPEGASGDLVTQP
                     LQLRVGSPAHTRWRLSWGDVGGLVALAGLAGYAGWRRWKKKLARRPASLPGQKDPFAA
                     ALSEIASARKAGNQKAFYGAVLSLLRQLTSERTGVPTAGPGARDLVPFADRLVSDENS
                     AEQLRDLLSRIDTILYAPATDDLDADMDVTELFVRNLVSENQIGKRERKDAS"
     gene            complement(8132..8671)
                     /locus_tag="P5179_06165"
     CDS             complement(8132..8671)
                     /locus_tag="P5179_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94837.1"
                     /translation="MNRVSVLATCALFAATRMACGRSPEALLKKGNAALLETTKPQPD
                     TATIDDGVKALREFTTRHMGHEHADSAYYLLGMIYGATQRNAEAGETFMTLVQNFPTS
                     RLRPKSLILASQAYENVKKYGHAKQCMQTLMAEYPDHEFVKGGSAKWLFLNIGKEPEA
                     WDQSFISDSSTARASTRPR"
     gene            complement(8742..9020)
                     /locus_tag="P5179_06170"
     CDS             complement(8742..9020)
                     /locus_tag="P5179_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRS94838.1"
                     /translation="MPTYEYQCESCGHRFERFQSIKADPLSECEICKGKVRRLISAGG
                     GLIFKGSGFYITDYRSEGYKAQSKRDNGGDSSTSSTVTSKETTAKTTT"
     gene            complement(9237..>9597)
                     /locus_tag="P5179_06175"
     CDS             complement(9237..>9597)
                     /locus_tag="P5179_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94839.1"
                     /translation="LNDTIVVFDRIREGLRIHGRRLTFGEILDKSINETLTRTIITSG
                     TTLLAVVALLIFAGQTLKDFALVLFIGVLTGTYSSIYIAGALVFEWQNRQQSRAAETA
                     RKRGAFAVNKTKKSATA"
BASE COUNT         2060 a         2932 c         2514 g         2091 t
ORIGIN      
        1 tcttcacggt tactcgctgt acgagagatg gttctatagg cgtcactgac ttgtctcgca
       61 atgtgtgcag ggatttcagg gtggaagaaa caagaatgac gtctgtcgta tagattgagt
      121 cactccgagg atattttaca taacagaagt tatcagtcgg tgtggcttta cctatgtcca
      181 gagtgtccgg tcgcatcgcg ctgtctacag gcttgatgat cagccgaaca agtggaggct
      241 tcagaccgaa gaatgcaaca ctgtcaggag aggatttcca cgcccgctgt ttccaggctc
      301 ttggcaggtt ctgcgcgaga gcacgccatt ccgtgctctc gcccggccac aggatcgggt
      361 ggacgatctg ccactcatcc gagcccactc gctccacaac cgttgtatca ggtttcggcg
      421 cattcggatt gttgataatc agggtgaacc ccgcgatctt ttccgtcggg atgcgcgtga
      481 ttttctgctc cagacgcttt tccagcgcga tcttatccag atgacgctcg ccaaaatacg
      541 cccagaatcc gagcgcgagg gctattacga ccagaatgat agtcctgatc cacttcttca
      601 tgccgttctt ctccgcctta gtgcatggac cgcgcgttca gagatcgcgt caacaggtcg
      661 cggggtgcga ttcattcgca ccccgctccg ctgatccccg tccggttttc tgcgttcaat
      721 tatccttgcc ggggccacga ctccgccggt accaccagat caagagaccg gcaacgaaca
      781 ccatcgccgg gtaccgccag acgcagaaca ggaacatgaa agtcccctgg ctcgcggtca
      841 tgacgatagg tttgtagccc ggctcccttg aatggatcgt aatcaggtct tcgtcttcgc
      901 tcaaccatgt aatgcagttg agcatgaaat ccacgttgcc ccgttcctgg ctcacgaccc
      961 tgtcggtcgc gaaatctgca tcgccgacca caacaagccg cgtttttgca ccttccacgc
     1021 cggtcgacac cgacggggga gcggtcgccg ccacggcaac cgagaaggga cccgacggct
     1081 tgccctctgc gctgtgcgcc tgccgcgggt tgcgcaacgc ctctttcacc tccgtgatcg
     1141 cgaacgacgt tcgtgagccc gcgaaaacaa tcggagtgcc agtacacgcc agcgtagggt
     1201 ttgcgcctgt ccacgtgatc tcccgaacct ggtagaaccc cgtcgcaatg cccttcgatt
     1261 cgagatcggc aacgatggcg tgcgaaggct catatttcat cgcacgcgcg tacatagact
     1321 gctgttcccc catgtacaga ccgcctgcat agggaaccag tccgcgttga cgctcgatga
     1381 ccatgttcgg gtgcacaaca acaccccatc tggcaacaac gttttccaga ccggtttcga
     1441 tcgaatccgg atccagcagg acaagagcac ttccaccttc cgccagatac cgttcgatcc
     1501 ggctgatttc caccggtgcg aatggcgctt ttggtccggg aataaccagc acagcacaat
     1561 ccggaggaac gtcggaatca ataagcttca acggcgcagt cgcgtaattc aactggcgga
     1621 gaagattggc caacataccc atctctcctt ccagcgtgcg ttcctggtgc cacatcgaaa
     1681 aatagattct cttctggccg gggcgcgtca ccttgattat tgcagttgtc aggtcgcgct
     1741 cctcgggccc aagaaccagc tcccgccggt cctcacattt gacaatgatg gtgatgtcgt
     1801 tggggttaat gttttcccga gcggcgatgt cagggtattt gtccgggtca acgaactcga
     1861 atgtgaaata gcgtgatgca gccgcgtatt tcttgagcat ctctgcgtac ccgggacgat
     1921 ttggatcaga gtcccggatg aatgcaatga ggtgcacgtc ctttctcagc cccttcatct
     1981 tcgccagggt ctggtctgaa aggctttcca gttgcgcctg tgtgatgtcg tacgtcgtcc
     2041 gcacgtggcg gttcgtgagg tagttcagca ccactgcaat cgcgagcgcg ctcactacgt
     2101 acacccagaa gttggcccct tgtgcactcg cccggccagt caacaccgat gcgatgccca
     2161 cgccaacttc tcccacgaac aaaccgagga acaacagaat agccgtgatg ctgtcgagct
     2221 tgaatgcgcc gatcacctcg cctttcacgt agatgatcgc gcaaagggca atcaaaacca
     2281 cgagcgcagc ggcaaacgcg acggattttc gaatccccag ccgcgaaagg aatgcaccca
     2341 gcaacaggcc tgttccgacc gcccagggaa tcccccaacc ccacaccgtg gcgctggcaa
     2401 gccacgcaac catgccatca acggcgagga gcaaacccac cagagcaata aacgggatag
     2461 gaatcgatcg caccatttac agcctccacc ttcgcgattc caacacccgg ttcgtgagca
     2521 acaacatgaa cccgatgaag gttatgtagt agacgatgtc gcgaagcgaa atcacaccgc
     2581 gcaggaacga gtctgcatgc ccgttgacgg caagatatct gaggaattcc gcggctgagt
     2641 agttgccgac gtagtccgcc gccgcaccga tgacaaggaa catgagcaag acactgaaac
     2701 tcactacagc ggcgactatc tgattttccg tcaacgcgga agcgaataca cccaccgaaa
     2761 tgaatgaagc gccgaggaaa agaaggccca gataacaact gagtattaac ccggtatcag
     2821 gccgtacccc ctcctccgca tacaggtacg caaacacgac aaagttcagc gtgagcacca
     2881 ggatacccgc gtacagtgca aatgacgcaa ggaacttccc tgccagcgtc tgccccggtg
     2941 tcacgggcga ggtgaaaaga agttcgatcg tgccgttacg gtattcttcc gaatacaggc
     3001 gcatcgtcag gatcggcata atgaacgtca gcaggaacgc catcacactg tacgtccggc
     3061 gaatcaccac aatgtcgacg ttgggaggag gaggcggatc ccgccgcatc tgctgggcct
     3121 gctgatatcg catgggatag tcagtcgtgt agcgattgag gaaattcatc gcgtcctgga
     3181 agaaataccc gcacagaatc agaaagacaa atgccatgac gtacgcgacg ggagagtgca
     3241 cgtacgaccg gagctccttg acgaaaacgg tccatgttcc tttcatcacg ccgcagcctc
     3301 ctccacgtcg ctactgatca cacggaggaa gatctcctcc agcgattcgc cgtggacgcg
     3361 ggtcaagttt tccagtgaat ccgcagcaca caacttgccc tggtgaatga tcgcgacagc
     3421 ttggcaggtc acgctcactt ccggcaatat gtgcgtgctc aaaatgaccg tgtgattacc
     3481 ggcaagcccc ttgatcagct cccggatttc gatgatctgg gctggatcaa ggccaatcgt
     3541 cggctcatca agtatgagca cctccgggtc atgcaccagc gcctgggcaa ggccggtccg
     3601 ttgacgatag cctttggaga gggtccctat gagctggttg accctgtctt cgagcccgca
     3661 cttctggagc gtggcagaca cggcctctgc acgcttattt cggggtacgc ctttcaacgc
     3721 cgcacagaaa tccacgtatt cacggacagt catgtcgttg taaaggggcg gagactccgg
     3781 caggtaaccg atgcgcctgc gcacctccat cgactgttcc tggacatcgt agcccgcaac
     3841 cagtgcagtt ccggaggttg gaggcaggta accagtgaga atccgcatgg tcgtcgtttt
     3901 gcctgccccg ttgggcccaa ggaacccaag gatctgcccc ttttccacgg tgaaactgat
     3961 atcgtccacc gcagtgaatg tcccgtactt cttggtgaga cctcggactt cgatcaattc
     4021 gctccctcct gtgtcggata ggttgcaaac gagtgaagct catccatgtt tttttcccgt
     4081 tttccccgga tttccggaga gacaatcttc cgtcatctca tgcgttcagg gtacagcttg
     4141 acttcctggc tggcgaaaag ctggtccacg agccgctgga aggcttcctg ttgcttcgcc
     4201 tggcgtgccg ccatctcgat ctggggtcgg atctgctgga ggcttggaac gcctccaccc
     4261 ggaacgcgag gcagttcggc ttgatgttct tcgtagaaac gccgcacgtc cgcttccgtt
     4321 atgtccggaa ggctcccgag ttgttcttca ccaatccgct gaacaagcat atcgcgcctg
     4381 atgcgatcaa gctggagttg gaactcggga ctatcggcat atcccctctt cactgccatc
     4441 tcataaagga tctccctgcc gacaacctgg cgcaggagtt cccgtttccg ctccatggtg
     4501 ctgaattgtt gacgcaattc cgggggaagc tgttccagtt cgcgcagcac ttcccgttcc
     4561 gaaatcttcc tgcctccgaa ctccgccacg accggccccg ccaacgaatc gggcggcaaa
     4621 gtcttcccct gggcaatctc tcgccgcatc agggatgcgt cagcactccg tccggttcgt
     4681 tcaaaacatg tgacaagccg tgcgcggact tcattcatct caggataatc tggggcgaaa
     4741 acccgtaatc tgaggtaata cacgagcgct gagtggtaat cgttcgctct gtcgaacagc
     4801 gcgtctgcca gcttgcgcat cgcgtccacg cggtccctgt cagacacggc cggcatttcc
     4861 aggtacttgt cgaaggcacg tatggcttcg gcgtagagct cccggttcag aagttcggtc
     4921 gcaaaaacac gctgcttctc ggggtcaacg ccagattttg aacagcccgt gccgaaggca
     4981 atcaggccga tcaccaaaag aaccctgatg tatctcatcc agatctccca tcggtacccg
     5041 ccgttcgcac ccttcacaca cgcgaaaagc aggtcgttct ccacgtcgca gacaacaggc
     5101 cgcgccggtc gcaggaaacg cgagcttcac attcgcttgg cacgcgccga aaagaacctc
     5161 acaagagggt ccacatagga cgtgtccgtc cacacgtcta cacaatcaac cccagtggtg
     5221 cggaacagcc gtttccgttg agcatgcctt gttcgcgcct gctgttcgta ccagtcccgg
     5281 aaacgtttgc ctgacgaatc cacaaccacg gattctccgg tctccggatc ctccagagcc
     5341 agcaacccgg ctcccttcag tgtgacctcg cgcgggtccg taagcgtgat cgcaatgaca
     5401 tcctgttttc gccgggcagc cttcagcgct ttctcaaaac ctgccgcctg gaaatcactt
     5461 atcaggaaga ccaccccgcg acgcgtcatg acgcgattca gaaaatcaag cgccccggcg
     5521 atgtcggtgc cgcgtccctt gtcagccccg tacgtgcgga atgcccacca cgaaaccacg
     5581 cccacgccgg cggcacccag aatggaaatt accgtctccg caagccactg gtaggtcgtc
     5641 tcggggatac caatcgccga aatcatcgaa gcagcggccg cgagaatcgc cagacttgcg
     5701 aggccaccca ttactgggtg cggccgactg cggaaaggtc gcggctcact ggtttccgtt
     5761 cgggaaaact gcgcaagaag ctctcgtatc acccgcagaa catgctttcg tccctttttg
     5821 ggcggaacaa acttctcgac tctgtcagtg aatactatga gacccaccct gtcgttgttc
     5881 ttggtggcgc tgaacgcgag aagggcgcac aactccacag cgatctggtt cttcgtctgc
     5941 tctaccgatc caaaatcacc cgacgacgat gcatccacca tcagcatcac cgtcagctct
     6001 cgttcctcat cgaatatctt cacaaaagga cgacgcatgc gcgccgtcac attccagtca
     6061 atcctgcgga cgtcatcgcc gggctgatac tcccgcacct cggcaaattc catcccttgc
     6121 ccttttagcg cggaatggta ttcgccgctc aaaagggtgt taaccacctg acgtgtctgg
     6181 atttcgatcc gacggacctt tttcaggatt tccttctgga tcatggcact tcaacggaat
     6241 cgaagacctt gctgacgatt gcttccgcgg tcacttcctc cgcctctgcc tcgtaggtag
     6301 gaatgacacg gtgccggaga acatccatcc cgatggcttt gacgtcctcc ggggtaacat
     6361 atccccggtg acgaagaaat gcgtgagcct tcgacgcgag agcgagccat atggttgcgc
     6421 gcggagaagc accataatcg acaaaatcgg tgtaatccgc caggccgtat tctgccggtt
     6481 ttcgggtcgc gcacacgatc tcgactatgt aattcttgat tttctcgtcc atgtaaatgc
     6541 gacgaaccag ttgacgggcg cggatgatgt ccgccggttc gacaacttca ttcacgtgcg
     6601 gaacatcgcc ttcggtcatt ctgtcgagta tttgacgttc ttctgccttg gaggggtagg
     6661 ttaccaccac tttcagcatg aaccggtcaa tttgtgcctc aggcaggggg tatgttcctt
     6721 cctgctcaat cgggttctgc gtggcgagaa ccaggaaagg atcctcaagg gcgaatgtct
     6781 ggtcgccgat ggtcacctgg tgttcctgca tcgcctccag catggcactc tgaactttcg
     6841 cgggtgcgcg gttcacttca tccgcaagca cgatattcgc gaaaatcggt cctttcttca
     6901 ccgtgaacgt cgacttcttc tgctcataga caggtgttcc taccacgtcg gcaggcaaaa
     6961 ggtcgggcgt aaactgaatc ctctgaaact tcgcatgtat cgtccgcgcc aggctgctta
     7021 ctgccagcgt tttggccagc ccgggaacgc cctccagcaa aatatgcccg tcgcaaagca
     7081 gtccgatcag catccgctca accatcgtgc gctggccgac aatcactttg ccgatttcgg
     7141 acacgatgcg ttctacgaac tcactttcgc gttcgatttc cgcatgaatc tcctgaatgt
     7201 ggctatcaag aggcatcctt gcgttccctt ttcccgatct ggttctcgct caccaaattc
     7261 ctcacaaaga gctccgtcac gtccatgtcc gcatccaaat catccgtcgc tggagcatac
     7321 aggatagtgt cgatgcggct caacaaatcg cgaagctgtt ccgcggaatt ctcatcggaa
     7381 acaagtcggt ccgcaaacgg gacgagatcc cgcgcaccag gccccgccgt cgggacaccc
     7441 gttcgttcgc tcgtcagctg cctcaggaga gaaagaacgg caccgtaaaa cgctttctga
     7501 tttcctgctt ttcgggcaga agcgatctcc gaaagggccg ccgcgaacgg atccttctgg
     7561 ccgggcaacg acgcgggtct tcgcgcgagt ttcttcttcc agcggcgcca tcccgcatat
     7621 ccggccagac ccgcgagggc aacgaggcct ccgacatctc cccaagaaag gcgccaccgc
     7681 gtgtgcgcgg gacttccgac gcgcaactgc aacggttggg ttaccagatc gcctgacgcg
     7741 ccttcaggcg tggtgaattg cacgcgaatc gcaccgacgg ccgcgtttcc ttcagattcc
     7801 ggctgaagaa cgtaggagaa ctcctgcgca gagtgttccc ccttctcatc cgcccaggag
     7861 cgcgttccac gtttactacc aatcacgatc aggttgtgga cacgcggtgc ctgcggtatt
     7921 acgagtgcga atctatccga aggcccctcc cattccgctt ttaccaccag cgtggccgtt
     7981 tcgtcaagcg cgacggtacc ccggtcgagt tgcgcctcga tgcgtatgtc atccgctggc
     8041 cacgcgtctc cgacggcgag aagaagcact acgcacagcg agagcaatgg tacgtgcaaa
     8101 atacccctgt gcggtacaac accagtgtaa ctcacctggg ccgggtcgac gcgcgtgccg
     8161 ttgatgagtc cgagatgaag ctctgatccc acgcttccgg ttctttcccg atgttcagga
     8221 aaagccattt ggctgacccg ccttttacga actcgtggtc cgggtattcc gccataagag
     8281 tctgcatgca ctgcttggcg tggccgtatt tttttacgtt ttcgtacgcc tgcgacgcca
     8341 gaatcaggct tttcggacgc aaccgactgg tggggaaatt ctgaaccagt gtcatgaacg
     8401 tttcccccgc ttccgcattc ctctgcgtcg cgccgtagat catgcccaga aggtagtacg
     8461 cgctgtccgc atgctcgtga cccatatgtc tggttgtgaa ctcccgcaac gctttcaccc
     8521 cgtcatcgat ggttgcggtg tcgggttgcg gtttggtcgt ctcaagaagg gccgcgttgc
     8581 cctttttcaa caacgcctcc gggctgcgtc cgcaggccat cctcgttgcc gcgaacaacg
     8641 cacacgttgc caacacggat acccgattca tttctctttc tctccgggga agaccgcatg
     8701 cgagaggaga agtgcttttt cctgcacgcg acatggccac gttacgtcgt cgttttcgcc
     8761 gtggtttctt ttgacgtcac cgtcgaggag gtactcgaat cacccccgtt atcgcgcttg
     8821 gactgtgcct tgtaaccttc gctgcggtaa tccgtaatgt agaaacccga tcccttgaag
     8881 atgaggccgc cgcctgcaga gatcagtcgc cgaactttcc ccttgcagat ttcgcactcg
     8941 gacaaaggat cggctttaat agattgaaac cgctcaaatc tgtgaccgca ggattcacac
     9001 tgatattcgt aggtaggcat atcgagctca attccctttc taattaacca tttcgggccg
     9061 attgccggag atgcccttcc ttcacatcat acccgcaacg ctgcgtaagc aacacggaaa
     9121 atcgagaggg tcaaaagggc aaaatacatg cccgatgaac cacaagcgag acagttgcgc
     9181 caaaaagaca gagttacgcc atagttgcag aggcgggcac gcccgttgtt cgccgcctac
     9241 gccgtggcgc tcttttttgt cttgttgacc gcaaaagcgc cccgtttcct cgccgtctcg
     9301 gccgcccggc tctgctgccg gttctgccac tcgaacacca gcgcgccagc gatgtagatg
     9361 gacgaatatg taccggtgag aaccccgatg aacagaacga gggcgaagtc tttcagggtc
     9421 tgtccggcaa aaatgagcag cgcaacgacg gccagaagag tggtgccact cgttatgatg
     9481 gtccgtgtaa gtgtttcgtt gatcgatttg tccagtatct cgccgaatgt cagccttctg
     9541 ccgtgtattc gcaggccttc acgaatgcgg tcaaatacca caatcgtatc gttcagg
//
LOCUS       DAOTNG010000096         9596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6551_length_9596_cov_5.539252, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000096 DAOTNG010000000
VERSION     DAOTNG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6551_length_9596_cov_5.539252"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1146)
                     /locus_tag="P5179_06180"
     CDS             complement(79..1146)
                     /locus_tag="P5179_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS94840.1"
                     /translation="MTKLPMQANAVLRHGIVIPAHPLALTRDRRLDERRQRALSRYYL
                     AAGAGGLAVAVHTTQFAIRKPKIGLLEPVLTLAAEEVARAQPGRDEPIIKIAGVVGRT
                     DQALAEAALAQRLGYDIALVSLAAFRRASDDTLITHCRAVAEVMPIMGFYLQPAVGGR
                     PLGQRFWARFAEIPGVVAVKVAPFNRYHTLDVVKGVAASGRLHEIALYTGNDDNILID
                     LLTPFRVAIPSGAMATARFVGGLLGHWAVWTKRAVHLLEVAHKLSTLDTIPRDVLTLA
                     AQVTEANAAVFDAANGYAGVIAGVHEVLRRQGLLAGTWCLDPNETLSPGQAREIDRIY
                     RLFPHLTDDEFVKEHLDEWLR"
     gene            complement(1143..>1567)
                     /locus_tag="P5179_06185"
     CDS             complement(1143..>1567)
                     /locus_tag="P5179_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94841.1"
                     /translation="SRRAVFDVLTACGVHPSRIYIAHADMNTSESESMWLAERGVRLV
                     MTNWDFPYHMDQNEARRLVRVLIDAGHLDKILISIDFALTMESRWTVGRFTWDNPDRT
                     SYAYLHTGVLPKLEDAVISKAELNVIMHDNPIEMVARK"
     assembly_gap    1568..1667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1668..2281)
                     /locus_tag="P5179_06190"
     CDS             complement(<1668..2281)
                     /locus_tag="P5179_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94842.1"
                     /translation="MSAVSPVDHADGTIDTALGRVSPGELEWVYPHEHLLISMREFAG
                     ESIANYPGNTEYARREIIAMLREVRAYGVNGLADPTPIGIGRDDAYVRLARSVSEATG
                     VHIFLSTGLYIPRHWPQWAIDETAAQIGERFCAEFERGIGETGARPTFLKAAVDGEVG
                     ANEEKALTACAYAHCRTGAAVHVHATRSRRAVFDVLTACGVHPSR"
     gene            complement(2278..3315)
                     /locus_tag="P5179_06195"
     CDS             complement(2278..3315)
                     /locus_tag="P5179_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS94843.1"
                     /translation="MNTLPEVIENETALDDLLTTPSPELVESVRRIPGDVMVLGVGGK
                     MGPTLAILLRRALEEAGSKARVIGVSRFSGPGLRERLHARGVETIPCDLLDPAQLATL
                     PHADTVIYLVGMKFGSTGAENLTWAMNTYLPGMVAQHFAGARMVALSTGNVYPLTPVM
                     GGGSRETDPVGPVGEYAQSCLGRERIFSYWTEKTGSPLTLVRLNYAAELRYGALLDIA
                     QKVHAGEPVDLTTGNFNCIWQGDANSIVVRALEIADSPPAVLNLTGPETLSVRRVAQQ
                     FGSLFGLEPVYSGEEAPTALLSNAGYCFRRFGYPRVPAQRMIEWTAHWVKIGGPTLNK
                     PTHFETRDGKF"
     gene            complement(3326..4084)
                     /locus_tag="P5179_06200"
     CDS             complement(3326..4084)
                     /locus_tag="P5179_06200"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS94844.1"
                     /translation="MRYVRFARGAKIGYGVLSGKTIRLLDRSFLQNARLTGEKVRLSE
                     VRLLAPVVPRTVMAIGLNYADHAKESNMPIPEEPFLFLKTPGCIIGPGDPIRLVVPEH
                     RNDYEAELVVVIRKRAHAVPAEKAHEFILGYTCGNDVSDRDYQKSDSQWMRSKGCDTY
                     GPIGPVITDEIDPHNVRIQTRLNGVVKQDSNTSQMIFPVQRLIEHVTRYVTLVPGDVV
                     FTGTPPGIGPMKAGDTVEVEIEGIGVLKNPVANA"
     gene            complement(4211..5014)
                     /locus_tag="P5179_06205"
     CDS             complement(4211..5014)
                     /locus_tag="P5179_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRS94845.1"
                     /translation="MQPNHVKEVLKAGGCAVGTMIHRFVYADVGALVKRAGVDFVLID
                     TEHAPLGMESVSLLCIATRAAGVVPIVRVPDSQYHLIARRLDAGALGIMMPRVKTRAE
                     VELIVESAKYPPLGGRGCGIRPVMLDFESTGSLSGDMDWINENTLVCIQIETREAMEN
                     LEEILSVPGVDATIMGPQDLSIALGTPGNHRSRAMELAVDRMIEVCDRYGVAPGIHIR
                     NTELLLDMKRRGMRLLACGFDEGFMLEAMSQTMNLLRGGDGPKAGETTV"
     gene            complement(5025..6716)
                     /locus_tag="P5179_06210"
     CDS             complement(5025..6716)
                     /locus_tag="P5179_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRS94846.1"
                     /translation="MIALVVLASMVFFAVAYRVYGRFLSRSVGIDDTQTTPAVEINDG
                     VDFVPAKSSLLIGQHFSAIAAAGPIVGPILAGLWFGWLPALLWVVVGAVFVGGVHDFA
                     SLIASVRHGSTSIGELLKQYLSPTAHVLFLGFVWLTLVYVIIAFTDITAQTFRVVAQG
                     EAFGPGVAVSSVLYLVLGVLMGVLLYRFKLNLTVATAIFLPLLVLIVWSGGHLPPSVV
                     TFMSSISSAQWNVVLLAYCFVASLLPMWLLLQPRGYLGGWFLYLTIATGLIGALFGGF
                     SIQYPAINMEGLRSLETGRMMFPVLFITIACGACSGFHAIVSSGTTSKQLRRESDARV
                     VGYGAMLLEGLVAVLALATVMMLPKGDAMLKGDPNLIYANGLARYLGLVGVDFSVALA
                     FALLAFSTFVYDTLDVCTRLARYILQELLGWKSRTGAYAATFITLLVPFLFLMATGEK
                     GYLVAWPIFGTSNQLLASLSLLALSVWLYKTGKPYLYAVIPMLFMLVMTLWSLVQLFT
                     PFLAGLPGLLSGAGMKTPVLVAGCCGLPLFVLTLWLVYESYSVLVLKRARENPSR"
     gene            complement(6732..7721)
                     /locus_tag="P5179_06215"
     CDS             complement(6732..7721)
                     /locus_tag="P5179_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94847.1"
                     /translation="MAKRQLWLLAALSLFTGIELWVMRILLPVLRRRVGVFPGMGVTE
                     ILQPVVAAVLMAGTAVLLLWMVFGRRSAGSEVPAVRKAVAGLVLLVGLMQVLRVAAGI
                     DDPWLDAARHVIAMCAIVLIAMDALTRGTTFGRASAGVGSLALFSSALYQSVILFYPT
                     SITARWLATFAETMSLFMPWALSVVVTRRYMDDLVAVAGFAFAVVVSVALATAAAPAI
                     HLIFAQATGFRHAVNPVLNVLTVGASVYFVIRSFRSEELPSQVPWAYIWLAASGIQML
                     QAHLQMAGLAALAVLAWTPPLVLPGQTRRWRFEKPEPVPAAAAVPREDEPPTP"
     gene            7802..8071
                     /locus_tag="P5179_06220"
     CDS             7802..8071
                     /locus_tag="P5179_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94848.1"
                     /translation="MEADNTAGASAARPARSDGKKKPEPCPAGIEDRCPIPRYRCREF
                     GEPGRHCFELFEEFEAFVLPEKSDDDQDEPARVLPFPRRRRRKSR"
     gene            8084..8314
                     /locus_tag="P5179_06225"
     CDS             8084..8314
                     /locus_tag="P5179_06225"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRS94849.1"
                     /translation="MDTLAKWFLKTGFFLMLFGTILVLLKRHHPAGKLPGDLVLRSGR
                     DRFVLPFGTIGLGVLVLTVYLNLLQRDSGSDS"
     gene            8317..9360
                     /gene="queA"
                     /locus_tag="P5179_06230"
     CDS             8317..9360
                     /gene="queA"
                     /locus_tag="P5179_06230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRS94850.1"
                     /translation="MPVDFQRTAYSYDFDPALIAQTPAVPRDSSRLLVFDRKTRAIRH
                     EVFRGLDQFLRPGDLLVVNTTRVMPVRLFPRERTAARPVELFLLRARSPRVWEALVKP
                     GRRIHPDSSLEWDDGTVATVIERLPDGSRVVEFSREVTPEWLEGHGKTPLPPYIRREA
                     SDEDRERYQTVYASAPGAVAAPTAGLHFTESLMERIAAAGVRKAELVLHVGIGTFRPV
                     RAEDIRQHAMESEYYEVSPDTLEQIRSTRASGGRIIAVGTTTVRTLETLADTGQIDAR
                     DTVAGWTRIFIHPPYRFRMVDALITNFHLPESTLIMLVSAFAGREQTLALYAEAVRLR
                     YRLYSYGDAMLIT"
     gene            complement(9333..>9596)
                     /locus_tag="P5179_06235"
     CDS             complement(9333..>9596)
                     /locus_tag="P5179_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94851.1"
                     /translation="RAVASGVYVYRLTAPQGVDEEDGVGPVGVRSRWRGGLRVPCTRG
                     TARTTACMRRFLCLPWDPIGLGRHGGARRHPALTLQVMSIASP"
BASE COUNT         1885 a         3098 c         2860 g         1653 t
ORIGIN      
        1 gaacagcccc agactggacc cgtcattccc gcgaaggcgg gaatccagaa ttacaggcgc
       61 tactcccggc ctgccgcact acctcagcca ctcgtcaagg tgttctttaa caaactcgtc
      121 gtccgtcagg tgcgggaaca ggcggtaaat gcggtcaatc tccctcgcct ggccgggcga
      181 aagcgtctcg ttcgggtcga ggcaccacgt tcccgccagc agcccctgcc tcctcagcac
      241 ctcatgtaca cccgcgatga caccggcata cccgtttgcc gcgtcaaaca ctgccgcatt
      301 ggcttccgtc acctgcgcgg cgagggtcag cacatcgcgc gggatggtat ccagagtgga
      361 gagtttgtgc gccacttcca ggaggtgaac cgcgcgtttc gtccataccg cccagtggcc
      421 gagaagtcct cccacgaaac gtgccgtagc catcgccccg gacgggatgg cgacccggaa
      481 gggggtgagc aggtcgatca ggatgttgtc atcgttgccg gtgtagaggg cgatttcgtg
      541 cagccgtccc gatgccgcga cacccttcac cacgtccagc gtgtggtacc ggttgaacgg
      601 cgcgactttc accgccacca cgccgggtat ttcggcgaaa cgcgcccaga atctctgccc
      661 cagcggccgt ccgccgaccg cgggctgcag atagaacccc atgatcggca tcacctcggc
      721 aaccgcgcgg cagtgggtaa tgagcgtgtc gtcactcgcg cgccggaacg cggcgagact
      781 caccagcgca atgtcgtacc ccagccgctg tgcgagcgcc gcctcggcca gtgcctggtc
      841 cgtccgcccg acgacgccgg cgatcttgat gatgggttcg tcgcggcccg gctgtgccct
      901 cgccacctct tccgccgcga gcgtcagcac cggttccaga agcccgatct ttggtttgcg
      961 gatggcgaac tgcgtcgtgt gcaccgcaac tgccagtcca cccgctccgg ccgcgaggta
     1021 ataccggctg agcgccctct gacgccgctc gtcgaggcgc cggtctctcg tcaacgcaag
     1081 gggatgcgcc ggaatgacga tcccgtggcg gagcactgcg tttgcctgca tcgggagttt
     1141 tgtcattttc gagccaccat ttcgataggg ttgtcgtgca tgattacgtt cagttcagct
     1201 ttactgatta cggcgtcttc cagcttgggc agcacgcccg tgtgaaggta cgcgtaggaa
     1261 gtacggtcgg ggttatccca ggtgaacctc ccaactgtcc agcgactttc catggtgagc
     1321 gcgaaatcaa tgctgatcaa tatcttgtcg agatggccgg catcgatgag cacccgcacc
     1381 aggcgcctcg cctcgttttg atccatgtga taggggaaat cccagttcgt catgacgagt
     1441 cggacgccgc gttccgcgag ccacatggat tccgattcgg acgtgttcat gtcggcgtga
     1501 gcaatgtaga ttcgcgacgg atgaacgccg cacgcagtca gcacatcgaa aaccgctcgc
     1561 cgcgaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc gacggatgaa
     1681 cgccgcacgc agtcagcaca tcgaaaaccg ctcgccgcga acgggttgcg tgaacatgca
     1741 ccgcagctcc cgtgcggcaa tgcgcatatg cgcatgcggt cagcgccttt tcctcgttgg
     1801 cgcccacctc tccgtcaacc gcggctttga gaaacgtcgg gcgcgcgccg gtttctccta
     1861 ttccgcgctc gaactcggcg cagaaccgct cgccgatctg tgctgctgtc tcgtcaatcg
     1921 cccactgtgg ccagtgccgc ggaatgtaca gtcctgtgct aaggaatatg tgtacgccgg
     1981 tggcctcgga aaccgatcgt gccaacctca catacgcgtc atcccggcca atcccgatgg
     2041 gcgtcgggtc ggccagacca ttcacgccgt acgcacgaac ctcccgcaac atggcgatga
     2101 tctcacgacg cgcatactca gtattgccgg ggtagttcgc gatggattcg ccggcgaact
     2161 cacgcatgct gatgagaagg tgctcgtgcg gatacaccca ctccagttcc ccggggctga
     2221 cacgcccgag ggcggtgtct atcgtgccat ctgcgtgatc taccggtgaa acggcgctca
     2281 aaacttccca tcccgcgttt cgaagtgggt cggtttgttc agcgtcgggc cgccgatttt
     2341 cacccagtga gccgtccact cgatcatccg ctgcgccggt acgcgcgggt acccgaaccg
     2401 ccggaaacag taccccgcgt tggaaaggag cgcagtgggt gcttcctcac cggaatacac
     2461 cggctcgagg ccgaaaagac tgccgaattg ctgcgcaaca cgccggacgg acagcgtttc
     2521 cggtccggta agattcaaca ccgccggggg agagtcggcg atttccagtg cgcggactac
     2581 gatgctgttg gcgtcaccct gccagatgca gttgaaattg cccgtggtga gatcaaccgg
     2641 ctcacccgcg tgcactttct gcgcgatatc cagcaacgcg ccgtaccgca actcggcggc
     2701 atagttgagg cggacgagag tcagtgggct gcccgttttc tctgtccagt acgagaagat
     2761 ccgctctctt ccgaggcagg actgcgcgta ttcgcccacc gggcccacgg ggtcagtttc
     2821 cctgcttccg ccacccatca cgggcgtcaa cggatacacg ttccccgtgg aaagagcgac
     2881 catccgcgcc ccggcgaagt gctgcgccac catgccgggg aggtaggtgt tcatcgccca
     2941 cgtgaggttc tccgcgcccg tggaaccgaa cttcatgccg acgaggtaga tcaccgtatc
     3001 ggcgtgcggc agggtggcca gttgggcggg gtcgagcagg tcgcagggta tcgtctccac
     3061 cccgcgggcg tgcagtctct ctcgcaaccc cgggccggaa aaccgagata caccgatcac
     3121 gcgcgccttg gagcccgcct cctccagcgc acggcgcagg agaattgcca gcgtcggccc
     3181 catcttcccg cccacgccca gcaccatcac gtcccccgga atcctccgga cgctttccac
     3241 gagctccggc gacggggtgg tcaacagatc atccagcgcg gtttcgttct caatcacctc
     3301 tggtagcgtg ttcacccatc tcctcctacg cgttcgccac gggatttttc agtacgccga
     3361 taccctcgat ttccacttcc acggtgtcgc cggccttcat cgggccgatg ccgggaggtg
     3421 ttcccgtgaa caccacatct cccggaacca gcgtcacata gcgcgtaacg tgctcaatca
     3481 ggcgttgcac cggaaaaatc atctggctgg tgttcgaatc ctgtttcacc accccgttca
     3541 accgcgtctg gatgcgtacg ttgtgggggt caatctcatc ggtgatgacg ggcccgatcg
     3601 ggccatacgt gtcacacccc ttggaacgca tccactggga gtcggacttc tggtagtcgc
     3661 ggtcggacac atcgtttccg caggtgtacc cgaggatgaa ctcgtgcgct ttctccgccg
     3721 gcacggcgtg cgcgcgtttc ctgatgacca ccaccagttc ggcctcgtag tcgttgcggt
     3781 gttccggaac gacgaggcgg atggggtccc ccggcccgat gatgcacccc ggagttttca
     3841 ggaacaggaa cggctcctcc gggatcggca tattggattc cttcgcgtga tcggcgtagt
     3901 tcaacccgat cgccatcacg gtgcgcggca ccaccggggc gagcagtcga acttccgaca
     3961 gccgcacctt ttctccggtc aaccgggcgt tctggaggaa cgatcggtcg agcagccgga
     4021 ttgttttccc ggaaagcacg ccgtagccga ttttcgcccc tctggcgaat cgcacgtatc
     4081 gcatttcgtc tcctttgatg aagggcggtt gccgccctcc acttcaatcc cactgtgatg
     4141 ctggtcgtgc caaccacgtt gtcttcacgc gggagcctgc ggaaatcaca gacccgcgca
     4201 tgcttcagac tcacacggtt gtctcgcccg ctttcgggcc atcacccccg cgcaggaggt
     4261 tcatggtctg cgacattgcc tcgagcatga acccttcgtc gaatccacac gcgagcagcc
     4321 gcattccgcg acgcttcata tcgagcaata gctccgtatt gcggatgtga atgccgggcg
     4381 cgacgccata tctgtcacac acctcaatca tccggtccac cgccagttcc atggcgcgag
     4441 accggtggtt ccccggtgtt ccgagcgcaa tggagaggtc ctgcgggccc atgatggtcg
     4501 cgtccacgcc cggaacgctg aggatctctt cgaggttctc catcgcttcg cgcgtttcga
     4561 tctggatgca gacgagcgtg ttttcgttga tccagtccat gtcgcctgaa aggcttcctg
     4621 tggactcaaa gtcgagcatc accggccgga tgccgcatcc acggccgcca agcggcgggt
     4681 atttcgccga ttccacaatc agctccacct cggcgcgggt tttcacgcgc ggcatcatga
     4741 tccccagcgc accggcgtcg aggcggcgtg cgatcaggtg gtactgcgaa tccggcacgc
     4801 ggacaatcgg caccacccct gcggcgcgcg tggcgatgca gagaagggac accgactcca
     4861 tgcccagcgg ggcgtgctcg gtgtcgatca gcacgaagtc gacgccagcg cgtttgacaa
     4921 gcgccccgac gtcggcgtac acgaaccggt ggatcattgt tccgaccgca cacccgccgg
     4981 ctttgagcac ttctttcacg tgattcggtt gcacgaaaga cctcctaacg cgaggggttt
     5041 tctcgcgctc gtttcagcac aagaacggag taggactcgt acacgagcca gagagtgagg
     5101 acaaagagcg gcaggccgca acagccggca acaagaacgg gcgttttcat tccggcgccg
     5161 ctcagaagac cgggcagccc cgcaaggaac ggcgtgaaga gctgcacgag agaccacagc
     5221 gtcatcacca gcatgaacag catcgggatg actgcgtaca ggtagggttt gcccgttttg
     5281 taaagccaga cggaaagcgc gagaagagag aggctggcga ggagctggtt actcgtgccg
     5341 aaaatcggcc aggcaaccag atatcccttc tctcccgttg ccatgaggaa caggaacggc
     5401 accagaaggg tgataaacgt ggctgcgtac gcgcccgtcc tggatttcca gccgagcagt
     5461 tcctgcagga tgtaccgcgc gaggcgggtg cagacatcca gcgtgtcgta cacgaacgtc
     5521 gaaaaggcca ggagcgcaaa cgcgagggcg acgctgaaat ccaccccaac cagcccgagg
     5581 taccgcgcca gcccgttggc gtagatgagg ttggggtcgc ccttcaacat ggcgtcgcct
     5641 ttgggcagca tcatgacggt ggccagtgcc agcacggcaa ccaggccctc gagcagcatt
     5701 gcgccgtacc cgaccacccg cgcgtccgac tcgcggcgga gctgtttgga cgtggttccc
     5761 gagcttacga tggcatggaa cccggaacag gccccgcagg caatggtgat gaagaggacc
     5821 gggaacatca tgcggcccgt ctcgaggctg cgcaggcctt ccatgttgat cgcgggatac
     5881 tggatcgaga atccgccgaa aagcgctccg atgagaccgg tggcgatggt gaggtagagg
     5941 aaccacccac cgagatagcc gcgcggctgg agcagcaacc acatgggaag gagcgacgcc
     6001 acgaagcagt acgccagcag caccacgttc cattgcgcgg aggaaatgga agacatgaag
     6061 gtcaccacgg acggcggaag atggccgcca ctccagacga taagcacgag gagcgggagg
     6121 aaaatcgccg tggcaacggt cagattgagc ttgaaccggt agagcagcac ccccatcagc
     6181 acgccgagca ccaggtacaa cacggaactg acggcaacac ccggcccgaa ggcttccccc
     6241 tgcgccacca cgcggaaggt ctgcgccgtg atgtccgtga atgcgatgat cacatacacc
     6301 agtgtgagcc agacgaaccc gaggaacagc acgtgtgcgg tcggcgagag atactgcttc
     6361 aggagttcac caatcgaggt agaaccgtgc cgcaccgacg cgatgaggct cgcaaaatcg
     6421 tgcacgccgc ccacaaacac cgcccccacc acaacccaca ggagggctgg cagccagcca
     6481 aaccagagcc cggccagaat cgggccaacg atggggcccg cggcggcgat ggccgaaaag
     6541 tgctggccga tgagcagaga ggactttgcc ggaacgaaat ccaccccgtc gttgatctcg
     6601 accgccgggg tggtctgggt gtcgtcaatt cccactgaac gggacaaaaa gcgcccgtac
     6661 acacgatacg caacggcgaa aaacaccatg ctcgccagca caacaagagc tatcatcctt
     6721 tttgaccatc ctcacggggt tgggggttca tcttcgcggg gaacagccgc ggcggcaggg
     6781 acgggttccg gcttctcgaa gcgccagcgt ctggtctgcc cgggcaaaac gagcggcggc
     6841 gtccacgcca gcacggccag cgccgcaaga cccgccatct ggaggtgtgc ctgcagcatc
     6901 tggattcccg acgccgcaag ccagatgtat gcccagggaa cctgagaagg cagttcctcg
     6961 gaacggaaac tcctgatcac gaaatagact gatgcaccta cggtcaacac attcaaaacc
     7021 gggttgacgg catggcggaa ccccgttgcc tgagcgaaaa tgaggtggat cgccggggcg
     7081 gcagccgtgg caagcgcgac agaaacgacc accgcaaagg caaacccggc aaccgccacc
     7141 aggtcgtcca tgtagcggcg cgtcaccacc accgagagcg cccacggcat gaacaggctc
     7201 atcgtctccg cgaatgtcgc gagccagcgt gcggtgatgc ttgtggggta gaaaagaatg
     7261 acgctctggt acagcgcgct ggagaagagc gcaagcgaac ccactcccgc gctggcgcgc
     7321 ccgaacgtgg ttccgcgggt gagtgcatcc attgcgataa gcacgatggc gcacatcgcg
     7381 atcacgtggc gcgcggcatc gagccacggg tcatcgattc cggcggcgac gcggagcacc
     7441 tgcatcaacc ccaccagaag cactaacccg gccaccgcct tccggaccgc cggaacttcg
     7501 ctccctgcag accgccggcc gaacaccatc cagaggagaa gcacggcggt acccgccatc
     7561 agcaccgcgg caacaacagg ctgaaggatt tccgttacgc ccattccggg aaacacgcca
     7621 acgcgacggc gaagaaccgg cagaagaatc cgcatcaccc acagttcaat gccggtgaac
     7681 aacgaaaggg ctgccagaag ccacaattgt cgttttgcca cagggtcctc ttcccgcgcc
     7741 ggggaacgcg cgctcgttat attcgtgtac aagatcaatc tgataacgaa agaggtcaac
     7801 catggaggca gataacaccg cgggcgccag cgccgcccgc ccggcaagat ctgacggcaa
     7861 aaagaaaccc gagccctgcc cggccgggat agaggaccgg tgtccgatcc cgcgttaccg
     7921 gtgccgggag tttggtgaac cggggcgcca ttgcttcgag ctgttcgagg agttcgaagc
     7981 cttcgttctt cccgaaaaga gcgacgacga ccaggacgaa ccggcgcgcg tactcccgtt
     8041 tccccgcaga cggcgcagaa aaagcaggtg aaggaggatc gtcatggata ccctcgcgaa
     8101 atggtttctc aaaacaggct tttttctcat gctcttcggg accatactgg tcctgctcaa
     8161 acgccatcac cccgcgggga agctgccggg tgacctggtc ctgcgcagcg gccgcgaccg
     8221 attcgtcctt ccgttcggca ccattggtct cggagtgctc gtgctgacgg tgtacctcaa
     8281 tctcctccaa cgtgactcag gcagcgattc gtgaacattc cggtcgattt ccagcgcacc
     8341 gcgtattcgt atgacttcga tcccgcgctg atcgcgcaga cacccgcggt gccgcgggat
     8401 tcctcccgcc ttctggtgtt cgacaggaaa acccgcgcca ttcgtcatga ggtattccgc
     8461 ggcctcgatc agttcctccg gccgggcgac ctgctggtcg tcaataccac gcgtgtgatg
     8521 ccggtgcggt tgtttccgcg tgagagaacg gctgccagac ccgtggagct ttttcttctg
     8581 cgggcacgtt cgccgcgtgt ctgggaggcg ctggtgaagc ccggcaggag aatccacccc
     8641 gacagcagcc tcgaatggga tgacgggacg gttgcaaccg taatcgagcg tctgcccgac
     8701 ggttcgcggg tggttgagtt ttcgcgcgaa gtgacgccgg aatggctcga aggccacggg
     8761 aaaacgccgt tgcccccgta catcaggcgc gaggcgagcg atgaagaccg ggagcgctac
     8821 cagactgtct acgcctctgc gccgggggcg gtcgccgcac ccaccgccgg cctccacttc
     8881 accgagtccc tcatggagcg catcgccgcg gcaggggtgc gcaaagcgga gctcgtcctc
     8941 cacgtcggca tcggcacgtt ccgtcccgtg cgggcggagg acatccggca gcacgccatg
     9001 gaaagcgaat actacgaagt ttctccggat acgctggaac agatacgctc aacccgggct
     9061 tccggcgggc ggattatcgc cgtcggaacg accacggtgc ggacactcga aaccctcgcc
     9121 gataccggtc agattgacgc ccgtgatacg gtggcgggct ggacacgcat cttcatccac
     9181 ccgccgtacc gcttccgcat ggtggacgcc ctcatcacga acttccacct gccggagtcc
     9241 accctcatca tgctggtgag cgccttcgcc gggcgcgagc agacccttgc gctgtatgcc
     9301 gaggcagtgc gcctgcggta ccgtctgtac agctacggcg acgcgatgct cataacctga
     9361 agcgtgagtg caggatggcg ccttgcgccg ccgtgcctcc caagtcctat cgggtcccat
     9421 gggaggcaaa ggaaacgccg catgcatgcc gtcgtacggg cggttccgcg tgtgcagggt
     9481 acacgaagac cgcccctcca ccgcgaacga acccctaccg gaccaacacc atcctcttcg
     9541 tccacgccct gcggcgcggt gagccggtag acatacaccc cgctggcaac cgcgcg
//
LOCUS       DAOTNG010000097         9567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6583_length_9567_cov_5.694281, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000097 DAOTNG010000000
VERSION     DAOTNG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_9567_cov_5.694281"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..727)
                     /gene="hisH"
                     /locus_tag="P5179_06240"
     CDS             complement(98..727)
                     /gene="hisH"
                     /locus_tag="P5179_06240"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRS94852.1"
                     /translation="MIAILDYEGGNLASVLRALDHTGFDACITWDFETIRRADHVILP
                     GVGAAKATMHHLRTRGLDKLIREEVIGGGIPFLGICIGVQVIFGSSEEEAAECLGILP
                     GSVRRFPSVCGGEQIKVPQIGWNQVRFSKPHPIFAGVEDGTSFYFVNSYYPVPASEEL
                     VYAKTTYGVEFASVVGKGNLVATQFHLEKSGVPGLRMLENFCRWDGSLS"
     gene            938..1012
                     /locus_tag="P5179_06245"
     tRNA            938..1012
                     /locus_tag="P5179_06245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:Gln,seq:ttg)
     gene            1072..1716
                     /locus_tag="P5179_06250"
     CDS             1072..1716
                     /locus_tag="P5179_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRS94853.1"
                     /translation="MNKFSLTVQARTRHGKQYAKQARRKGLVPATLYGAGANPVSVQA
                     DARTLTRVFHQARTTAIIDVTVEGGETCQALFREPVFHPLNDTLLHVDLMRVSSTSMV
                     RVEVPVEIVGSSKGVLAGGILDHVLHALEIECQALAIPERIPVDVTQLEIGDHITVGE
                     LKLEGLRILTDTDQTIVTISAPRVEEEAPAEEEPTAAEPELVKRKKATEEEEES"
     gene            1731..2381
                     /gene="pth"
                     /locus_tag="P5179_06255"
     CDS             1731..2381
                     /gene="pth"
                     /locus_tag="P5179_06255"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRS94854.1"
                     /translation="MIGLGNPGPSYARTRHNVGWFVLDSLIPLDRWVRGDGPFLFAGY
                     PAVGSECVLVKPLTFMNLSGEAAVAVCRQFTVGPADLLVLLDDVNLPIGDLRLRRSGS
                     AGGHNGMASIGEALQTQDVPRLRIGIGRPSEPGGLVDYVLSPFSEEEEPVVRQAAIEA
                     GGIVRAFCSGGFEGAAELYSRWKAGSRSEPVAGSEKTDKQSAGMNEEASGDPAKTE"
     gene            2532..5159
                     /locus_tag="P5179_06260"
     CDS             2532..5159
                     /locus_tag="P5179_06260"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRS94855.1"
                     /translation="MSESMLLSIVLGISVFGLIVAWALAKWVLKKDVGSQAMQDISNA
                     IKEGAEAFLRRQFKTIIWLAAAFAVLLFIGYGFLRNHMPTDPVTTATGLAFWITLSFV
                     LGALCSLFAGYIGMWVSIRANIRTATGAMKNLNEAVQIALRGGAVSGLMVVSMSLLGI
                     AGLYTAVKLTTAVDPTKIPLLIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVE
                     AGIPEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILAASMAKAVPDAS
                     PLWILGVMMFPLIARAFGIIASIVGIMMVKVKEHEDPMSSLNRGYYWAVVLAMIGFGI
                     ASYWLLNHPSAPSAWWHFFLCGIIGVITSIAFVYITQYYTESKYRPVKSIADASKTGP
                     ATNIISGIAVGLECTAMPVIVISIALLASYYLGASSGFAHAGLFGTAVATMGMLATAA
                     YILAMDTFGPITDNAGGIVEMSQQPEEVREKTDRLDAVGNTTKALTKGYAIGSAALAA
                     FLLFSAYIDEVNIYLQGKGMILSTVDIAKPEVFVGALLGAMLVFLFSAFAIRAVGHAA
                     QYVIQDVRDQFKEKPGILAGTEKPDYGRCVDIVTKGALREMVLPGLLAVLMPIVVGVV
                     FRFMVFGGDAWRPLGIVSFKADIPAVEQVAGAEALPEAAVAEENTAAPAEGEMMTEAP
                     AEEVAPAVACAAESVMTYGAANTFAFAADTAYLSRPLVDEVWAITKKKYVDEDSLIAI
                     GQSRIRATSVEVVRSYNADSALSIQPASVVVTGVKSASAVVKSPVGAETVAALLMVGT
                     IAGILMALFLNNGGGAWDNAKKHIEAGYLGGKGSDPHKAAVVGDTVGDPFKDTAGPSL
                     HVLIKLLSTITLVLAPLFI"
     gene            5399..5848
                     /gene="rpsF"
                     /locus_tag="P5179_06265"
     CDS             5399..5848
                     /gene="rpsF"
                     /locus_tag="P5179_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRS94856.1"
                     /translation="MPLYETTLVIDSLLEETAITEKVQKYVDLVTKAGAVDLQVDRRG
                     VRKLAYDIKGKDGQWRTQADYTFLFYEAPGTAVNILEKQLLLDEDMLRTMTVRYDRMP
                     PATPADLNAIEAVDSSRDDDEERGRGDRERVKDTGVDVDALDEEEEG"
     gene            5853..6086
                     /gene="rpsR"
                     /locus_tag="P5179_06270"
     CDS             5853..6086
                     /gene="rpsR"
                     /locus_tag="P5179_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRS94857.1"
                     /translation="MPREKKIRVKVCKFCEAKITHIDYKDEKLLRRFLSDRGKIIPRR
                     MSGVCERHQRMLTTAVKRARQIALLPYVSEMIK"
     gene            6112..6630
                     /gene="rplI"
                     /locus_tag="P5179_06275"
     CDS             6112..6630
                     /gene="rplI"
                     /locus_tag="P5179_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRS94858.1"
                     /translation="MKVILRDNVETLGRAGDVVSVKDGYARNYLIPRSLAYPATERNL
                     RVWEDEKRKFLVRVSKETQEAERVKAALEPVHLVIPMQAGEEGRLFGSVTNRDLAEAL
                     KTHGFDVDHRHIVIEEPVRVLGAFEFFVKLPHGVTATMKGEVVDAAGQKAPETVEDTE
                     TKEPEGTIEQSE"
     assembly_gap    6701..6800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6801..7549)
                     /locus_tag="P5179_06280"
     CDS             complement(<6801..7549)
                     /locus_tag="P5179_06280"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRS94859.1"
                     /translation="MDSSRELTIQDALNEALRTEMRRDERVFCMGEDIAIYGGAYGVT
                     KGLLDEFGAERVRDTAISEAAIIGAGVGAAMTGMRPVVELMYVDFAGIAMDQIYNQAA
                     KIRYMFGGKAKVPLVIRTQGGTGKQIAAQHSNSLEAWFLHIPGLKTVMPSTPYDAKGL
                     LISAIRDDNPVMFIEHKAIYNEKGPCPEGDYTVPIGKAAIRRPGTDATIVSYSRMALR
                     SMSAAETLAGEGISCEVIDLRTISPWDTHAVV"
     gene            complement(7564..9024)
                     /locus_tag="P5179_06285"
     CDS             complement(7564..9024)
                     /locus_tag="P5179_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRS94860.1"
                     /translation="MTEIRPIKGIKTVEDFRAYVEARGLDLHCEDSIAPAASSPLCEP
                     LHVYGRTIGNRWAVHPMEGWDGTTDGGATELVIRRWRRFGESGAKLIWGCEAMAIRPD
                     ARANPNQLIISSHTESDLARLRGVLLHAHRERFETTDDMMIGFQLTHSGRFCKPWDKG
                     KFESRVAYRHPILDRKFGVTSDAQVFRDDEIGPLVEDYIKAAKIARNVGADFVDIKHC
                     HGYLLHEFLGAKTRNGPYGGSFENRTRLLREIAAGIRAEVPGMLLGVRLSAFDTVPYK
                     PDPEKSEGGVLGPGIPEDYSGCLPYVYGFGVNETDPVQYDLTETCLFLELLRELDIRL
                     VNITAGSPYYNPHIQRPAIRPPSDGYAPQEDPLVGVARHINVVKYLKARFPDLVLVGT
                     GYTYLQEYLPHVAQYYLRHQHVDSVGIGRLVLTYPHFFVDATTKGVLDARIICRTLSD
                     CTTGPRKGLPSGCYPLDEFYKTSEYGARLRELKKAR"
     gene            complement(9039..>9567)
                     /locus_tag="P5179_06290"
     CDS             complement(9039..>9567)
                     /locus_tag="P5179_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS94861.1"
                     /translation="RENRLGRFKEEMRRAAEATCDFYCQNSPTDGIPYWDTGAPGLAA
                     LGYYLDRPADPFNDYEPVDSSAAAIAVQGLLRLGKWLSDHGNSETGARYWQTGLTVLH
                     NLLAEPYLSTDEKHQGLLLHSVYHRPNGWDYVPEGRRVPCGESSMWGDYHLREALLFV
                     QRVALRLPYLTFYTP"
BASE COUNT         1957 a         2746 c         2807 g         1957 t
ORIGIN      
        1 aacgcgttac gaatccacca accggccatc ccaccggcgc ccagcatgag acaggtcttc
       61 ttttccatgg agcatccttt ccgttccgac gcgccggtta gctcagcgag ccatcccacc
      121 ggcagaagtt ttccagcatg cgcaaaccgg gcacaccgct tttctcgaga tggaattgcg
      181 tcgcgacgag attccccttg ccaactaccg acgcgaactc gacgccgtac gtcgtttttg
      241 cgtagacaag ctcttcagac gctggcaccg ggtagtacga gttcacaaag tagaacgacg
      301 ttccgtcctc cacgccggca aaaatcggat gtggtttgct gaaacgcacc tggttccacc
      361 cgatttgcgg aactttgatt tgttcgcccc cgcaaaccga agggaaccgc cgcaccgaac
      421 cgggcaggat gccaaggcat tccgccgcct cctcttcact cgagccgaag atgacctgaa
      481 ctccgatgca gataccgagg aaggggatcc cgccccctat cacctcttcc cgtatgagtt
      541 tgtccagacc tcgggtgcgg aggtggtgca ttgtcgcctt cgccgctccc actccgggca
      601 ggatcacgtg gtcggcgcgg cggatcgtct cgaaatccca cgtgatgcag gcatcaaacc
      661 cggtgtggtc cagcgcacgg agaacacttg cgaggtttcc gccttcgtaa tcaagaatcg
      721 cgatcattgg tctttcccga tagagatacg tcagaacgtg tccggttccc gtaagaaact
      781 agcgggaatc gagtaccggc gcaatcggat tacgcgacgg gatatgactg cggggtggcc
      841 gtgtcaggct tgacagagag gccaaataac gttaaaattt actattcccc aagaacaggg
      901 ttacattgcc cgtgaatccc catgaagacg cggtctctgg ggcgtcgtca aatggcaaga
      961 cacaggattt tggatcctgc acttggaggt tcgagtcctc ccgccccaac caccatctgg
     1021 gattattcat cgaccaaaac agacggcttt ctcacgtaag gacaacccag catgaacaag
     1081 ttcagcctca cggttcaggc gcgcacccgg cacggaaagc agtatgccaa gcaggctcgt
     1141 cgcaaaggac ttgttccggc gacgctttac ggggccggtg cgaacccggt ttccgtgcag
     1201 gccgatgccc ggacgctcac cagagtattt caccaggccc gcactaccgc aatcatcgac
     1261 gtgactgtcg aaggcgggga aacctgccag gcccttttcc gcgagccggt gtttcacccg
     1321 ttgaatgata cgttgctcca tgtggacctc atgcgtgtgt cgagcacgtc aatggtgcgg
     1381 gtcgaagtgc ctgtggaaat cgtggggagt tccaaaggcg tgcttgccgg aggtattctc
     1441 gaccacgttc tgcacgcgct cgagatcgag tgccaggccc tggccattcc cgagcgcata
     1501 ccggttgatg tgacacaact cgaaataggc gatcacataa cggtcggaga actgaaactc
     1561 gagggattgc gaatcctcac ggatacagac cagaccatcg taaccatttc cgcgcctcga
     1621 gtggaagagg aagcgcctgc agaagaggag ccgactgcgg cggagccgga actggtgaag
     1681 cggaagaagg cgaccgagga agaggaagag tcgtagacag cgtgaaagct gtgatcggtt
     1741 tggggaaccc cgggccttct tacgcacgga ctcgccacaa cgtgggttgg ttcgtcctgg
     1801 attccttgat tcctttggat cggtgggtac gtggagacgg accgtttctt tttgccggtt
     1861 acccggcagt tggttccgaa tgcgtcctcg tgaagccgct tactttcatg aatctgagcg
     1921 gggaagcggc ggttgcggtg tgtcgtcagt tcacggttgg tccggcggat ctcctcgtgc
     1981 ttcttgacga cgtcaacctg ccgatcggtg atctgcgcct caggagaagt ggttccgcgg
     2041 gcggacacaa cggaatggcg tcgatagggg aagccctcca gacgcaggat gtgccgcggt
     2101 tacgaatcgg cattggccgt ccatcggaac ctggcgggtt ggttgattat gtcctctcgc
     2161 cgttttcaga ggaagaggaa cccgtcgtcc ggcaggccgc aatcgaggcg ggtggaattg
     2221 tccgcgcctt ctgttcgggc ggttttgaag gcgcggctga actgtacagc cgatggaaag
     2281 ccggttcccg gtcggaaccg gtggcgggtt cggaaaaaac ggataagcag tcggccggaa
     2341 tgaacgagga agcatcaggc gatccggcaa aaacggagtg aagtgaagta agaggagcat
     2401 agagttctgc gtcatcggat gaagggagga cgaggccctc gcaaacgatg acgaaatgag
     2461 agggaggccg ttgggcgcgc ctcccgttgg ggttggtgga aaactccatt cctgggaaag
     2521 gagctcttcg aatgagcgaa agcatgttgc tgagtatcgt ccttggcatc agcgtgttcg
     2581 ggctgatcgt cgcctgggcg cttgccaaat gggttctcaa gaaagacgtg ggaagccagg
     2641 cgatgcagga tatctcgaac gccatcaagg aaggcgccga ggccttcctg cgaaggcagt
     2701 tcaaaacgat tatctggctc gctgcggcct tcgcggtgtt gcttttcatc ggttacggat
     2761 tcctacgcaa ccatatgccg acggatcccg tgacaaccgc caccggtctg gcgttctgga
     2821 tcactctttc gttcgtgctc ggagctctgt gttcgctttt cgccgggtac atcggaatgt
     2881 gggtcagcat tcgcgccaac atccgcacgg cgacgggcgc gatgaagaat ctcaacgaag
     2941 cagttcagat cgcactccgc ggtggagcgg tctccggcct gatggtcgtt tcgatgagcc
     3001 ttctgggcat cgccgggttg tacaccgcgg tcaaactgac taccgcagtt gatcccacca
     3061 agattccgct cctgatcgtc ggattcggat ttggtgcgtc ctttgtcgcc ctctttgctc
     3121 agctcggcgg cgggatttat acgaaggccg cagacgtcgg cgccgacctc gtgggtaagg
     3181 ttgaggcagg cattccggaa gatgatcccc gcaaccctgc cgtcatcgca gacctcgtgg
     3241 gagacaacgt cggtgactgc gccggccgtg gtgcagactt gttcgagtct accgcggcgg
     3301 agaacatcgg tgcgatgatc cttgcggcca gcatggcaaa ggccgtcccg gacgccagtc
     3361 cgctctggat tctcggcgtc atgatgttcc cgcttattgc ccgcgccttc ggtatcatcg
     3421 cttccatcgt cggaatcatg atggtgaaag tgaaagagca cgaagacccg atgtcgtcgc
     3481 tcaaccgtgg ctactactgg gccgtcgttc tggcgatgat cggcttcggc atcgcaagtt
     3541 actggctgct gaaccatccg tcggcaccaa gcgcgtggtg gcacttcttc ctgtgcggca
     3601 tcattggcgt cattacctca atcgcattcg tctacatcac gcaatattac acggaatcga
     3661 agtatcgccc ggtgaagtcg atcgccgacg cgagcaaaac cgggcccgcc acgaacatca
     3721 tctctggcat cgccgtcggt ctcgagtgca ccgcgatgcc cgtcatcgtg atttccatcg
     3781 ctcttctcgc gtcctactat ctcggtgcga gttcgggctt tgcgcatgcc ggcctcttcg
     3841 gaactgctgt tgcaacgatg ggtatgctgg ctaccgcggc gtatatcctc gcgatggata
     3901 ccttcggacc cattactgac aacgccggcg gcatcgtgga gatgagccag caaccggaag
     3961 aggtgcgtga aaagaccgat cgccttgacg ccgttggcaa cacgaccaag gccttgacaa
     4021 aaggatacgc gattggttct gcggcactgg ctgctttcct gcttttcagc gcatacatcg
     4081 acgaggtcaa catctacctc cagggcaagg gcatgattct ttccaccgtg gatatcgcca
     4141 aaccggaagt cttcgtcggc gctctcctcg gtgcgatgct tgtgttcctc ttctccgcgt
     4201 tcgcgatccg tgcagtcggc cacgccgccc agtatgtcat tcaggacgtg cgtgaccagt
     4261 tcaaggagaa gccgggcatt cttgcaggaa cggaaaaacc cgattacggt cggtgcgttg
     4321 atatcgttac gaagggtgca ctccgtgaga tggttctccc gggtctcctg gccgtgctta
     4381 tgccgatcgt agtgggtgtg gtgttccgct tcatggtgtt cggtggagac gcgtggcgcc
     4441 cgctgggtat cgtcagcttc aaggctgata tcccggcagt tgagcaggtt gcgggtgcag
     4501 aggctctgcc ggaagctgca gtcgcggaag agaatactgc tgctcccgcg gaaggcgaaa
     4561 tgatgacgga agcgcccgcg gaggaagtgg ccccggccgt cgcgtgtgcc gcggaatccg
     4621 tgatgacgta cggcgcagcg aacaccttcg cgtttgccgc agacacggca tacctctcga
     4681 ggcctctcgt cgacgaggta tgggcgatca ccaaaaagaa gtacgtggac gaggatagcc
     4741 ttatcgcaat cgggcaaagc cgcatacgcg ccaccagcgt cgaggtcgtc cgttcgtaca
     4801 atgcggattc ggcgctttcc atccagccgg catccgttgt cgttaccggt gtgaaaagtg
     4861 ccagtgcagt ggtgaagtcg ccggttggcg ccgaaactgt tgcggcgctg ctcatggttg
     4921 gcacgatagc cggaatcctc atggcgttgt tcctgaacaa tggcggcggt gcatgggata
     4981 acgcgaaaaa gcacatcgaa gccggttacc tcggcggcaa gggttctgat ccgcacaaag
     5041 ccgccgtggt gggtgacact gtcggcgatc ccttcaagga caccgcaggg ccttcgttgc
     5101 acgtgctgat caagctcctt tcgacaatta ctctggttct cgcgccattg ttcatctagt
     5161 gagcgcgcgt gcagggaatg cagagggcgg ggcgcgaacc ccgccctctg cattttgcgg
     5221 gtcacagcgt cgggaggttc cacctgtccc gcgctgttca acaattggca aagaacgatc
     5281 gagataacta tctttataag tcttttctgg gaatcgctcg ccggcgtttc ccctctcggc
     5341 ttccacccgc gacggcggag gcttgactaa acgtcgatcc atcgggagga ttgcgcgtat
     5401 gccgttgtac gaaacaaccc tggtgataga ctccttactt gaggagactg ctatcaccga
     5461 gaaagtgcag aaatacgtag acctcgtaac aaaggccggg gcggtagact tgcaggttga
     5521 ccgccgcggg gtccgcaaac tggcgtatga catcaaaggc aaagatggcc agtggaggac
     5581 gcaggccgat tacacgttcc tcttctatga agccccggga accgctgtca acatcctcga
     5641 gaagcagctt ctccttgacg aagacatgct gcggacgatg accgtccgct acgatcgcat
     5701 gccgcctgct acacccgcag acctcaacgc catcgaagca gtcgacagct cgcgtgacga
     5761 tgacgaggaa cggggccggg gagaccgcga acgcgtgaag gacacgggtg ttgacgttga
     5821 cgcccttgac gaagaagagg agggctgaac agatgccacg ggaaaaaaag atccgggtga
     5881 aggtgtgcaa gttctgcgaa gcgaagatca ctcacattga ctacaaggat gagaagcttc
     5941 tgcgcagatt tctttctgat cgcggcaaaa ttatcccgcg cagaatgtcc ggcgtgtgcg
     6001 aacgccatca gcgcatgctg accactgcgg tgaaacgcgc gcgccagatt gcgctgcttc
     6061 cgtacgtatc cgaaatgatc aagtagacag gccccgatac gaggtaaaac gatgaaagtg
     6121 attttgagag ataacgtgga aacactcggg cgggcgggag acgtcgtttc ggtaaaagac
     6181 gggtatgcgc gcaattacct catcccccgc agcctggcgt atccagccac cgaacggaat
     6241 ctgcgcgtgt gggaagacga aaagcgcaag ttcctcgtgc gcgtttcgaa agagacccag
     6301 gaagccgaac gcgtcaaggc agcgctcgaa ccggtgcacc tggtcattcc gatgcaggca
     6361 ggtgaggaag ggcgcctttt cggctccgtt acgaaccgcg accttgcgga agcactgaaa
     6421 acgcacggtt ttgacgtaga ccaccgtcac atcgtcatag aggaacccgt tcgcgtgctg
     6481 ggagcgttcg agttcttcgt caagttgccc cacggggtta cggcgacgat gaaaggcgaa
     6541 gtcgtcgacg cagccggtca gaaagccccc gaaaccgtag aagacacgga aacaaaggag
     6601 cccgaaggaa ccatcgaaca gagcgagtga tccgagggct tcatcggcaa agaatggcgc
     6661 tcaggaggtg ttcttgagcg ccttcttttt attggagagt nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn accactgcgt gggtatccca cgggcttatg gtccgcaaat
     6841 caatcacttc gcaggagatg ccttctccgg cgagcgtctc cgctgcggac atgctcctca
     6901 gcgccatccg cgagtaactc acgatcgttg cgtccgttcc ggggcggcga atggcggctt
     6961 ttcctattgg caccgtgtaa tcgccttccg gacagggtcc cttctcgttg taaattgcct
     7021 tgtgctcgat gaacatgact ggattgtcat cccggatcgc ggaaatcagc agccctttcg
     7081 catcgtacgg tgtggacggc atcacagtct tcaggccggg tatgtgcaga aaccacgcct
     7141 cgaggctgtt ggagtgctga gcggcgatct gttttccggt gcccccctgc gtacggatta
     7201 caagcggcac cttcgccttt ccgccgaaca tgtaccggat tttcgccgcc tggttgtata
     7261 tctggtccat ggcgataccg gcgaagtcca catacatcag ttccacgaca ggtcgcatgc
     7321 cggtcatcgc agcgccaacg ccggctccga tgattgccgc ttcgctgatc gcggtgtcac
     7381 gaacgcgttc cgcgccgaac tcatccagaa gacccttggt aaccccgtac gcgccgccgt
     7441 agatcgcgat gtcctcaccc atgcagaaaa ctcgttcgtc gcgacgcatc tccgtccgca
     7501 acgcttcatt gagagcgtcc tgaatcgtga gttcacgtga ggaatccatc tcacccccga
     7561 agatcaacgt gctttcttga gctcccgcaa ccgggcgcca tattcacttg ttttgtagaa
     7621 ctcgtccaaa gggtaacagc cactcggaag gcccttcctc ggacccgtgg tgcagtccga
     7681 aagtgtgcgg cagatgatcc tcgcatcaag cacgcccttc gttgtcgcgt caacgaagaa
     7741 atgcgggtac gtcaatacca gccgcccgat tccaactgag tccacgtgct gatgccgcag
     7801 atagtactgc gccacgtgag ggaggtactc ctgcaagtag gtatatccgg ttcccacaag
     7861 aaccagatcg gggaacctcg ctttcaggta tttgacgacg tttatgtgcc gtgcgacgcc
     7921 gacaagcggg tcttcctgcg gcgcgtaccc gtcgctggga ggccgaatcg ccgggcgttg
     7981 gatatgcgga ttgtagtacg gacttccagc agtaatgttg acgagccgga tgtccagctc
     8041 gcggagaagc tcaaggaaca gacacgtctc cgtaagatcg tattgaacag ggtccgtttc
     8101 gttgacgcca aagccgtaca cgtagggcag gcaccccgaa tagtcttccg ggatcccggg
     8161 tcccaggaca ccgccttcgc tcttctccgg atctggctta tagggaaccg tatcgaacgc
     8221 actcagccgt actccgagga gcatcccggg aacttccgcc cggattcccg ccgcaatttc
     8281 ccgcaggagc cgggttctgt tctcaaacga accgccgtac ggtccgttgc gcgtcttcgc
     8341 gcccagaaat tcgtgcagca ggtatccgtg gcagtgtttg atgtcaacga aatcagcgcc
     8401 tacgtttcgc gcgatcttcg ctgccttgat gtagtcctcc acgagcgggc cgatttcgtc
     8461 atcccggaaa acctgtgcgt cgctcgtaac accgaacttc ctgtcaagga tcgggtgccg
     8521 gtaggctacg cgggattcga atttcccttt gtcccagggt ttgcagaagc gccctgaatg
     8581 ggtcagttgg aagccgatca tcatgtcgtc ggtggtttcg aaccgctccc ggtgtgcatg
     8641 aagcagaacg ccccgcagcc gcgcaagatc agattccgtg tgagacgaga tgatcaactg
     8701 gttggggttt gcgcgggcat ccggacgaat cgccatcgcc tcgcaacccc agatcagttt
     8761 ggcgccgctt tcgccgaagc gacgccatcg ccggataacg agctccgttg caccaccatc
     8821 agttgtccca tcccaccctt ccatcgggtg cactgcccag cggtttccga tggttcttcc
     8881 gtacacatgg agaggctcgc aaagcggcga tgaagccgcc ggggcaatcg aatcctcgca
     8941 gtgcaggtcc agcccgcgcg cttcgacgta cgcgcggaag tcctccactg tcttgattcc
     9001 cttgataggg cgtatctcgg tcacggcagg agttcccgtc acggggtgta gaacgtcagg
     9061 tatggcagtc tcaacgcaac gcgctgaacg aacagcagag cttctcgcag atggtaatca
     9121 ccccacatgc tcgactcccc gcagggaacc cgacgccctt caggtacgta gtcccagccg
     9181 tttggccggt gatacaccga atggaggagc aacccctgat gtttctcgtc ggtcgaaagg
     9241 tagggttccg caagaaggtt gtggagcacg gtgaggcccg tctgccagta acgcgccccc
     9301 gtctcgctgt ttccgtgatc agaaagccat ttccctagtc ggagcagccc ctgaacggcg
     9361 attgcggcgg cagagctgtc caccggctca tagtcgttga acgggtccgc cggacggtcg
     9421 aggtaatacc ccagcgcggc aagacccggc gcgcccgtat cccagtacgg aatcccatcg
     9481 gtggggctgt tctggcagta gaagtcgcac gtagcctctg ccgctctccg catctcctcc
     9541 ttgaaccgcc ccagtcggtt ctcccga
//
LOCUS       DAOTNG010000098         9434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6686_length_9434_cov_4.719373, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000098 DAOTNG010000000
VERSION     DAOTNG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_9434_cov_4.719373"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1251)
                     /gene="coaBC"
                     /locus_tag="P5179_06295"
     CDS             complement(22..1251)
                     /gene="coaBC"
                     /locus_tag="P5179_06295"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRS94862.1"
                     /translation="MLRGRRIVIGVTGGIACYKTCELVRLCVKAGAEVKVVMTEHATE
                     FVAPLTFETLSGHDVVVGMYGRNRAMSTEHIAMARWAEIIVIAPATANILAKAAHGIA
                     DDFLSTMIIATYSPVMFVPAMNTAMLKAPATQRNIPLLLESGYHITPTGKGELADGEV
                     GEGRMIEPREIMHHLVRAIPRAGILAGKRVLVTAGPTEEPVDPVRVFTNRSSGKMGVA
                     LAEMAAGMGAHVTFVHGPLTVPPPIGADCMAVGTAQQMMDAVLARFPETNVLVMAAAV
                     ADYRPRTVADQKIKKTTDTLTIEMERTTDILASVARKKKPGQIVVGFAVETEAEKAEK
                     RARERLQKKSLDMIALNVIGEKGVGFGTDTNRVTLFCRDGSKTELPLMEKREIARTMW
                     ERIAALQGTGPQTKNEA"
     gene            complement(1349..3148)
                     /locus_tag="P5179_06300"
     CDS             complement(1349..3148)
                     /locus_tag="P5179_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94863.1"
                     /translation="MATITWSVFVTAPHITRYGRTAESRARLIKDVLDIGAKKVFLEV
                     WRGTVETDPDILRMLRDECKAAGLEVATGIMPVRGRDITLEGIPQWDSDTAIGGNSRW
                     GADICYSRPWSREALRKAMQTGAELFDEFIIDDALCTQCTCPRCREAKGDRTWTQFRR
                     DLLLEVCRETLVGTCRKANPNIKMILKFPQWYDQLATFGYDTARQPAEFDATWIGTET
                     RDPDTPDFGFTPQYEACFNVRWHKAAEPNLEGAWFDYIECDTDLYVEQAYQSLLGGVR
                     NLTLFCYGDPLFRGKGHLLAALKEEQPRLEKLASRLDGQQPIGITAIRPHSPAPKNDG
                     YVFDGLGMLGMPLVPRADFPELLPPTVLLTDHVAGSPELTPFVSEVVNSGGTVFFTAS
                     LLAEHDDDEELKTLAGFGGDGWSQRNHWFSSHFMVGDRAVEASAPVEFRFDLRPSTAK
                     VLASVSGVAHHRAERIPVVTRQVHVSGGAVVVLNVFGASPADYRMDENLNVPIVLQMQ
                     NYPEPVVNVIQEQVALATGQAFLAPARVGYYPYISGDVVLENFTDEKVFAVPVGEQIP
                     LDARELLGVAAIEEKDGQRGVLLPPRSVALIGR"
     gene            3496..>4006
                     /locus_tag="P5179_06305"
     CDS             3496..>4006
                     /locus_tag="P5179_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRS94864.1"
                     /translation="MAVPRTVSEERLLAIICHLVERAGVPATDARRCARILVTADLRG
                     IPSHGVRMLPGKIHRVLGGAFNPNPRITKVAEIGSITVLDADRAIGMVAGAVAMETAI
                     SLARGNGISWVSVRNSNHFGAAGSYVMMAVEEGMVGFCFSNCGPIMAVYGTKSRCIGN
                     NPMAIGAPHR"
     assembly_gap    4007..4106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4107..4679
                     /locus_tag="P5179_06310"
     CDS             <4107..4679
                     /locus_tag="P5179_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRS94865.1"
                     /translation="TKSRCIGNNPMAIGAPHRAFPVVLDMALSISSFGRIHTMKRNGD
                     PVPDEWYAVPPEPGKDAVLRAMGGPKGSGLAIMVEILTSVVAGGAVPWDMRPFKSTEK
                     NDGTTHTMIALDVRRLVPEDQYEETLDRFVEQLKTAERVEGVNEVILPGELEWRSTLK
                     KRAEGIPLDADVVESLDNVAAFLGTAVPWE"
     gene            complement(4728..5669)
                     /locus_tag="P5179_06315"
     CDS             complement(4728..5669)
                     /locus_tag="P5179_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HRS94866.1"
                     /translation="MTKTALHRLSIASVVALTVAGCGLDPVSPAPREQLIIQAFLSPG
                     APVDNIRLTRTMDPATYYEGIAQTPVSGAVVVLRHGVSVDTLPELATGIYGNPSLIVQ
                     AGQTYSIAVTHGVHSLAAETTVPTPVTVSAQTNGAPSRYVPSADTLVFPREYAYPDSF
                     PDRATISPKPFKIFWTPSVGIEGYQIAITARDTSGTGLLRLGDYNDWKAGDYRNARKR
                     QREVTLTYPAFPDSLSSDLFWVGFKYQGRYDIIVFAMDRNFYNYFMSSGGGNSGADYD
                     TGVTNYVRGGLGVFGSYAADTVRTFIKPEWLPSAERP"
     gene            complement(5672..7948)
                     /locus_tag="P5179_06320"
     CDS             complement(5672..7948)
                     /locus_tag="P5179_06320"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94867.1"
                     /translation="MPQRFVRIAALVASLAAVLVPRAAEAATVSGYVRDKSSGESLPY
                     VQVFFEDLRTGAITNLAGYYAIPNVPPGNHVLRVAFLGYQDYTKEITVNGGVLVHNIE
                     LAEEAIEVAAVQVEAERVTAPSLTIAPSRTTLRMPDFRTAPALAEPDPIRVVQTLPGV
                     LSLSDFSVGLYVRGSTPDQNLLLLDGVSLYNVSHLFGLFSAFPADAVKSSELLKGGFP
                     AQYGGRLSSVLTINTDEGNKRTMVGKGGVSLISSRLTMEGPFAKGSWLVSGRRTYLEP
                     ILKLASRWNDTFGNLGYNFYDVQGKTHQVFSHEHQLSIAGYAGDDNLRFSDQTFDSQL
                     LWGNRSLSATWSYVPVDRLFFRTTGAIARYRSRLQLGIEDFGVVERNRLFDWTVKSDA
                     TVFLTEHNTVETGFEFICHTMNYTADFDKQTYNSFSIRTHHASAYAQTTLRPWAFLSV
                     QPGVRANYFDNGGYVDIDPRFSARYQIGENTYLKGAVGRYSQYLFRVAREFQGINFLS
                     DLWFTADSTAEPSHAWQYVAGLETKLTPDIDLTLEGYYKDFEMLAEFNSDAQTPMSAG
                     QMLRRGDGEAYGLEFGLQKRAGRHKGWISYTLGWTTRTIEGINVDNYGNPQPYYPKFD
                     QRHAANLVYGFDVTPRWTLNARYAFASGQGYTPVIGRYSISDPIFVSEQLYRDRLNSR
                     RLPYYSRLDIGLRGKFRGWGVTWMPFMEVINVTNRKNIFNRYWDDGNPDATPPEPGKE
                     KNFPQLPFLVTLGVDLEF"
     gene            8175..8786
                     /locus_tag="P5179_06325"
     CDS             8175..8786
                     /locus_tag="P5179_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRS94868.1"
                     /translation="MTNCGCEIEASNRGEHRTLIAVLVINALMFVLEFGFGVVAQSTG
                     LISDSLDMFADASVYAISLYAVGRSAALKARAAALSGFSQILLGAGASVEVARRFVFG
                     SDPVSSAMVAIGLVALVANVTCMMLIYRHRLGEVHMKASWIFSKNDVIANVGVIAAGI
                     LVGITGSRYPDLVIGLAIAVVVARGGVQILAEARAAGDGRTEC"
     gene            8853..>9434
                     /locus_tag="P5179_06330"
     CDS             8853..>9434
                     /locus_tag="P5179_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS94869.1"
                     /translation="MTQEKPWVESDVWPSGRYLAAYLGIGVLVGLASALLGIGGGVLI
                     VPLLTGFLGVPIRRAVAISIVTVLLISFIGVARELQRGTTLPWLLVAVLAVGAQGGVW
                     LGGKVHDRISDRALRHVFMVVLLLTAAKMANLGGLGPARGIILLEDPVHWVAWVLVVG
                     ALAGLLSVLVGIGGGIVVVPGMALLIADIDFRVI"
BASE COUNT         1790 a         2690 c         2879 g         1975 t
ORIGIN      
        1 gattcagcgg gatccacgaa cttacgcctc gtttttcgtc tgcggacccg tgccctgaag
       61 cgccgcgatc cgttcccaca ttgtccgtgc gatctctcgc ttttccatca acgggagttc
      121 agttttcgag ccgtcccggc agaacaatgt cacccggttt gtatccgtgc cgaagcccac
      181 gcctttctcg ccgatgacgt taagcgcgat catgtcgaga gacttcttct gcaggcgttc
      241 ccgcgcccgt ttctccgcct tttcagcctc ggtttccacg gcgaagccga ccacaatctg
      301 ccccggtttc ttcttcctgg caaccgatgc gagaatgtcc gtagtgcgct ccatttcgat
      361 tgtcagcgtg tcggtcgttt tctttatttt ctggtcggcg accgttctcg ggcggtaatc
      421 ggccactgca gccgccatta cgagcacatt cgtttccggg aatctggcaa gaaccgcatc
      481 catcatctgt tgagcagttc ctaccgccat gcagtcagcg ccaatcggcg ggggtaccgt
      541 caacggacca tggacaaacg ttacgtgtgc ccccatcccc gcagccattt cggcgagcgc
      601 gacgcccatt tttcccgatg atcggttggt gaacacgcgt acagggtcga cgggctcttc
      661 tgtcggtccc gccgtcacca gcacccgctt tcctgccaga atgccggcgc ggggaatcgc
      721 cctcacgaga tgatgcatga tctcacgcgg ctcgatcatc cgcccttcgc ctacttcgcc
      781 gtcggccagc tcacctttcc cggtcggagt gatgtggtaa cctgactcca ggagcagagg
      841 gatgttccgc tgagtggcgg gcgccttcag catggcggtg ttcatcgcgg gcacaaacat
      901 gaccggcgaa tacgtggcaa tgatcatcgt gctgagaaag tcatccgcga taccgtgtgc
      961 cgctttcgcc aggatgttcg ctgttgccgg cgcaatgact atgatttcgg cccaccgtgc
     1021 catagcaatg tgttccgtgg acatcgcgcg gttgcgcccg tacataccca cgacgacgtc
     1081 gtggccggac aaggtttcga atgtgagcgg tgcaacgaat tccgtcgcgt gctccgtcat
     1141 gactaccttg acttccgcgc ccgccttcac gcagagacgc acaagttcgc acgttttgta
     1201 acaggcgata ccgcccgtca ccccgatgac aatccgtctc ccccgaagca tgtcccggtt
     1261 cctccttcca accccctgcg cacccgacgg aacattcggg ggatacgccg ccgcgcaccc
     1321 cccgttcccg tcgcaagaac acgcggagtt acctcccgat aagagccaca gaacgcggcg
     1381 gcagaaggac accccgctgt ccgtctttct cctcgattgc ggcaactccc agcaattccc
     1441 gcgcatccag cgggatctgt tcgcccaccg gcaccgcgaa cactttttcg tccgtgaagt
     1501 tctccagtac gacatcgccg ctgatgtacg ggtagtaccc cacccgggcc ggagcgagga
     1561 aggcctgccc cgtggccagc gcaacctgct cctgaataac gttcacgacg ggttccggat
     1621 agttctgcat ctgaagcacg atcggcacgt tcaggttctc gtccatccgg taatccgcgg
     1681 gagatgcgcc gaacacgttc agcaccacaa cggcaccacc ggagacgtgc acctggcgcg
     1741 taaccacggg aatgcgttcc gcgcgatgat gtgcgacccc gctgacggag gccagcacct
     1801 tcgcggtgct tggcctgagg tcgaaccgga actcaaccgg cgcggaggcc tccacggccc
     1861 tgtcgcctac catgaagtgg gagctgaacc aatgatttct ctgggaccag ccatcgccgc
     1921 cgaagccggc gagcgttttc aattcctcgt cgtcatcgtg ttccgcgagc aacgacgcgg
     1981 taaagaagac ggtccctccg gaattgacaa cctcggacac gaacggggtg agctccgggc
     2041 tccctgcgac atggtcggta aggagtacgg tgggcgggag aagttccggg aaatcggccc
     2101 gcggcaccag cggcattccg agcatcccga gtccgtcgaa gacgtacccg tcgtttttcg
     2161 gcgcgggact gtgcgggcga atggcggtga tgcctatcgg ttgctggccg tccagtcgcg
     2221 aggcaagttt ttccagccgt ggttgctctt ccttcagggc cgccagcagg tgtcccttcc
     2281 cgcggaaaag cgggtcgcca taacagaaaa gcgtcagatt ccgcacgcct ccgaggagcg
     2341 actggtacgc ctgctcgacg taaaggtcgg tgtcgcactc gatgtagtcg aaccacgcgc
     2401 cctcgagatt cggttcggcc gctttgtgcc agcgcacgtt gaagcaagct tcgtactggg
     2461 gcgtgaaacc aaaatcggga gtgtccgggt cgcgtgtttc cgtgccgatc caagtggcat
     2521 caaattcggc gggctggcga gccgtatcat acccgaaggt agcaagctga tcataccact
     2581 gcgggaattt gaggatcatc ttgatgttcg gattcgcttt cctgcaggtg ccgacgagag
     2641 tttccctgca cacctccaac aacagatcgc gccggaattg agtccatgtg cggtcacctt
     2701 tcgcttcgcg gcagcgcggg cacgtgcact gtgtgcagag ggcgtcgtcg atgatgaatt
     2761 cgtcgaacaa ctcggcaccg gtctgcatcg ccttccgaag cgcctcgcgg ctccacggcc
     2821 ggctgtagca gatgtcggcg ccccaacggc tgtttccgcc gattgcggtg tcgctatccc
     2881 actgcgggat accttccagt gtgatgtcgc ggccacggac cggcatgatg ccggtggcca
     2941 cttccagtcc agccgctttg cactcgtcgc gcagcattcg caggatgtcc gggtcagttt
     3001 cgacagtgcc gcgccagacc tccagaaaga cctttttcgc gcctatatcg agaacatcct
     3061 tgatcagccg tgcgcggctt tccgcagtcc gaccgtaccg ggtgatgtga ggggcagtga
     3121 cgaataccga ccaggtgatt gttgccatga taatcctcgc gagttttcct gacagtggat
     3181 tccgaaggga cattgtgaat gataatgact accctgttcg ggagcgaagg tcagggaggc
     3241 gggcaacacg gtggcgtcag cggaatcgct gtacctgcgt cgggcccttc gacactctac
     3301 tgagaggcac ccggagactg gcacacgagg gccggtcgtt cccaaggcat agacaggcaa
     3361 acgcctgcac catccgcgtt caatcccgcc gcaggaaccc cgaaagcctg ccgtcattgc
     3421 gcgcaggaaa gggggccggg tatcttggaa cagaactccc gttcaacgcc acgcctgtct
     3481 gtgagggaat gcgttatggc agtgccccga accgtgtccg aagagcgcct gcttgcgatc
     3541 atttgtcatc tggtagaacg tgctggcgtg cctgccactg atgcgcgccg gtgtgcccga
     3601 attctcgtca cagcggacct tcggggtatc ccgtcgcacg gcgtgcggat gcttcccggc
     3661 aagatccatc gcgtgctggg gggagcgttc aacccgaacc cacggatcac gaaagtcgcg
     3721 gaaatcgggt caattacagt tcttgatgcc gaccgggcaa tagggatggt ggcgggcgcg
     3781 gtagcaatgg agacggcgat ttcgctcgcg cgggggaacg gcatctcctg ggtgagcgtg
     3841 cgcaactcaa atcacttcgg cgccgcgggc agctatgtaa tgatggcggt cgaggaaggg
     3901 atggtcgggt tctgcttttc caattgcggc cccatcatgg cggtatacgg aacgaaatcg
     3961 cgctgcatcg gcaacaaccc gatggccata ggagctcccc acagggnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnacga aatcgcgctg catcggcaac aacccgatgg
     4141 ccataggagc tccccacagg gcctttcccg ttgttctcga catggcgctg agcatttctt
     4201 cgttcggacg gatccacacg atgaaacgaa acggagatcc tgtgcccgac gaatggtacg
     4261 ccgtaccgcc agaacccggc aaggatgcgg tgttacgggc gatgggaggg ccgaaaggga
     4321 gcggccttgc catcatggtg gagattctta ccagtgtggt cgcgggggga gcggtcccgt
     4381 gggacatgcg gccgttcaaa agcaccgaga aaaacgacgg aacgactcac acgatgattg
     4441 cgctcgatgt gcggcggctg gttccggagg accagtacga ggagactctt gaccggttcg
     4501 tggagcaact gaaaactgcg gaacgcgtgg agggggtgaa cgaagtaatc ctgccgggag
     4561 agctggaatg gcgttctacg ctgaagaaac gagcggaagg aatccctctt gatgcggacg
     4621 tggtggaatc gctggacaac gttgcagctt tccttggaac ggctgtgccg tgggaataga
     4681 caacgcggct ccgtgtccgg taatcttttg ggcccgtgct gtttctttca gggccgctcc
     4741 gccgacggca gccattccgg ttttatgaaa gtccgcactg tatccgcggc gtaggaaccg
     4801 aacaccccga ggccccctct cacgtaattg gtgacgccgg tgtcgtaatc cgcgcccgag
     4861 ttgcctcccc ctgaggacat gaagtagttg tagaaattcc tgtccatggc aaacacgatg
     4921 atgtcgtacc tgccctggta tttgaacccg acccagaaca gatcgctgct cagggaatcg
     4981 gggaacgcgg ggtaggtgag tgtcacctcg cgctgccttt tgcgggcgtt gcggtaatcg
     5041 cccgctttcc aatcgttgta gtcgcccagc cgcaacagcc ccgtgccgct ggtatcccgt
     5101 gcggtgattg caatctggta cccctcaatt ccgacggagg gcgtccagaa gattttgaag
     5161 ggtttgggcg aaattgtggc gcggtcaggg aagctgtccg gatacgcgta ctccctggga
     5221 aacaccagtg tatctgcgga cgggacgtag cgcgagggcg cgccgtttgt ctgcgctgag
     5281 acggtgacag gcgtgggaac ggtagtctct gcggcgagcg agtggacccc gtgcgtaacg
     5341 gcgatcgaat acgtctggcc ggcctgaacg atcagcgaag ggttcccgta gatcccggta
     5401 gcaagttctg gaagtgtatc taccgatact ccgtgacgga ggacaacgac agcgcccgaa
     5461 acaggtgtct gagcgattcc ctcgtaatag gttgccgggt ccatcgtccg cgtgaggcgt
     5521 atgttatcca ccggtgcacc gggggaaagg aaggcctgaa taatgagctg ttccctcggc
     5581 gcgggactga cggggtcaag tccgcaccct gcaaccgtaa gagccacaac agatgcgatc
     5641 gaaagtcgat ggagagctgt tttcgtcatg gttagaactc cagatccacg ccgagggtga
     5701 caaggaaggg caattgcggg aagtttttct ccttccccgg ttccgggggc gtcgcatccg
     5761 ggtttccgtc atcccagtat cgattgaaga tatttttccg gtttgtcaca ttgattactt
     5821 ccatgaacgg catccacgtc actccccaac cccggaattt gccacgcagg ccgatatcca
     5881 gccgcgaata gtagggaagg cgtctgctgt tcagacgatc ccggtagagc tgctcgctca
     5941 cgaagatggg gtccgagatt gagtagcgtc cgatgaccgg agtgtatccc tgtccggatg
     6001 caaacgcgta ccgtgcgttg agcgtccatc tcggggtgac gtcgaaaccg tacacgagat
     6061 ttgcggcgtg ccgctggtcg aatttggggt aatagggctg cgggttgccg taattatcca
     6121 cgttgatgcc ttctatggtg cgggttgtcc atcccagcgt gtaagaaatc catcctttgt
     6181 gcctccccgc gcgtttctgg aggccgaatt ccagcccgta cgcttcgcca tcccctcgcc
     6241 gcagcatctg gcctgccgac atcggtgtct gggcatcgct gttgaactcc gccagcattt
     6301 cgaagtcctt gtagtacccc tccagcgtca gatcgatatc cggggtcagt ttcgtttcca
     6361 accccgccac gtactgccac gcatggctcg gctcggcggt ggaatccgcc gtgaaccaga
     6421 gatcggacag gaagttgatc ccctgaaact cgcgcgcgac acggaagagg tactggctgt
     6481 acctgccgac cgcccctttg agataggtgt tctcgccgat ctggtagcgc gcggagaagc
     6541 gaggatcgat atccacgtag ccgccgttat cgaagtagtt ggctctcacg cccggctgaa
     6601 cggagagaaa cgcccacgga cggagggttg tctgcgcgta ggcactggcg tggtgggttc
     6661 ttatcgaaaa cgagttgtat gtctgtttgt cgaaatccgc ggtgtaattc atcgtgtggc
     6721 agatgaattc gaaccccgtc tcgacggtgt tgtgctcagt caggaagacc gtagcatcgc
     6781 ttttgaccgt ccagtcgaac aagcgatttc gttcgacaac cccgaagtct tcaattccga
     6841 gctgcaatcg gcttcgatac cgggcgatgg caccggtggt gcggaaaaac agccggtcaa
     6901 ccggaacata cgaccacgtt gcggaaaggg accggtttcc ccagagcaac tgggaatcga
     6961 acgtctgatc ggagaaacgc aggttgtcgt ccccggcgta gccggcaatg gagagctggt
     7021 gctcgtgtga gaacacctgg tgcgtttttc cctggacgtc gtagaaattg tagccgaggt
     7081 tgccgaacgt gtcattccag cgggaggcca gcttcaggat aggctccaga tacgttcttc
     7141 gtcctgagac aagccacgaa ccctttgcga aagggccctc catggtgagg cgtgagctaa
     7201 tgaggcttac gccgcctttc cctaccattg tgcgcttgtt gccttcatcg gtgttgatcg
     7261 tcagcaccga cgacagccgg ccaccgtact gagccgggaa cccgcctttc agcagttcgc
     7321 tggatttcac ggcatctgcc ggaaacgcgg agaagaggcc gaacaggtga gaaacgttgt
     7381 aaagtgagac gccatcaagg agaagaagat tctggtccgg tgtgctgccg cgcacgtaga
     7441 ggcccacgga aaaatcggaa agcgacagaa cacccggcaa cgtctgcacc acgcgaattg
     7501 gatccggttc tgcaagcgcg ggtgcggtgc ggaaatccgg catccggagc gtcgtccggc
     7561 tcggtgcgat ggtgagagag ggtgcggtta cccgttcagc ctcgacctga accgcggcga
     7621 cctctattgc ctcttcggca agctcaatat tgtggaccag cacaccaccg ttgacggtga
     7681 tttccttcgt gtaatcctga tacccgagga aggcaactcg cagcacgtgg ttgccgggag
     7741 gcacgttcgg gatggcatag taacccgcaa ggttcgtgat cgcgcctgtg cggagatcct
     7801 caaagaaaac ctgaacgtac gggaggcttt cgccggagga tttgtctcga acgtacccgc
     7861 ttacggttgc cgcctcggcg gcccgtggaa caagcacggc ggcgagcgat gcaacgagcg
     7921 cggcaatccg aacgaaacgt tgtggcattc tctatccatt cgtggtgaag aatcagtctt
     7981 ccgggttcgc acgctgggtg ccgtctgtca tcccaactta cctataccgg cgaacttgcg
     8041 tgaccggttc cagaaaatga gcggtttgca cgttttttct gcagcggttc tgggagggtg
     8101 ggaaccggtt gatgttctgt gccagattag gtggagggca atcctgtcag aacaaccttc
     8161 cggaaggtac gattgtgacg aattgcgggt gtgagatcga ggccagtaat cggggagaac
     8221 accggaccct tattgccgtt cttgtgatca acgcgttgat gttcgtgctc gagttcggct
     8281 tcggcgttgt ggcgcagtcc acgggcctca tatcggattc actggacatg tttgcggacg
     8341 cttctgtcta cgcgatcagt ctctacgccg ttggcaggag tgccgcactg aaggcaaggg
     8401 ctgccgcact gagcggcttc tcgcagattc tcctgggagc aggcgcgtcc gttgaggtcg
     8461 cacgacgatt cgtgttcgga agcgatcctg tcagcagtgc gatggtggcg attggcctcg
     8521 tggcgctggt ggcaaacgtt acctgcatga tgctcattta tcgtcaccgt ctgggcgagg
     8581 tccacatgaa agcgtcgtgg atcttttcga agaacgatgt gatcgcgaac gtgggagtca
     8641 tcgcagcggg gattctcgtt ggaataaccg gttctcggta ccccgatctg gtgatcggcc
     8701 tggcaatcgc ggtggttgtt gcgcgcggcg gcgtgcagat tctggcggag gcacgagcgg
     8761 ctggcgacgg acggacagaa tgttgacccg gtgaatagtc ggactctgga ttccgtgcga
     8821 gagcggtacc cgaattgatt ggcggaggct ggatgacaca ggaaaaaccg tgggtggagt
     8881 ctgatgtctg gccctcgggc cggtatctgg cagcatatct cggcatcggc gtactggtcg
     8941 gtctggcaag cgcgcttctc gggatcggtg gaggcgttct gattgtgccc ctgctgacag
     9001 gtttcctcgg tgttccgatc cgtcgagcgg ttgccatctc catcgtaacg gtcctgttga
     9061 tttccttcat tggcgttgcg cgagaactgc agagaggcac cacactgccc tggcttcttg
     9121 tggcagtact cgcggtgggt gcgcagggag gcgtatggct cggagggaag gtccacgatc
     9181 gcatctcgga cagggcgttg cgccatgtgt ttatggtggt cctgctgctt acagccgcga
     9241 aaatggcgaa tctgggaggt ctcggtccgg cccgggggat catcctgctc gaggatccag
     9301 ttcactgggt tgcgtgggtg ttggttgtgg gagcgctcgc cggtcttctt tccgtcctgg
     9361 tggggatcgg gggtggaata gtggttgttc ccggtatggc gctactcata gcggatatcg
     9421 atttccgcgt gatt
//
LOCUS       DAOTNG010000099         9410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6712_length_9410_cov_5.556173, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000099 DAOTNG010000000
VERSION     DAOTNG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_9410_cov_5.556173"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="P5179_06335"
     CDS             complement(<1..927)
                     /locus_tag="P5179_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRS94870.1"
                     /translation="MIPYDFRTLEPQWRAWWEENKINEAKDDSSRPKYYCLDMFPYPS
                     GEGLHVGHWRNYVLSDVWSRYQRLRGYQVLHPIGWDAFGLPAENFAIKHKIHPRISTA
                     RNIANMKRQLKIIGAMYDWSREISTCDPEYYRWTQWIFLRMYKRGLAYRSTAPVNWCP
                     SCRVGLANEEVVAGGCERCGTAVVKRDLVQWYLRITAYAERLLRDLERLDWPERVKTM
                     QANWIGRSEGARITFTAVHPGNQTTYPIDVFTTRPDTLFGATYMVLAPEHPLVPALTM
                     ASRRKEVEAYREQARNVRDVDRTAATRPKTGVP"
     gene            complement(973..2277)
                     /gene="asnS"
                     /locus_tag="P5179_06340"
     CDS             complement(973..2277)
                     /gene="asnS"
                     /locus_tag="P5179_06340"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRS94871.1"
                     /translation="MWNERPTIEEIARYEGQEIVLKGWLNNMRSSGKLHFLQVRDGTG
                     VMQCVVFKGNVTPEVFETAAALKQESSLVVRGQIRADARSPIGFEMDVVDLQAIHVPE
                     VDYPISPKEHGTAFLMDHRHLWLRSSRQQAILRIRAELIRQIRAYFDANGFLLLDAPI
                     FTPSACEGTTTLFETDYVDGSKAYLTQSGQLYMEAGATAFGRVYCFGPTFRAEKSKTR
                     RHLTEFWMVEPEVAHIGLDEDMDLAEDFLVSIVQGVVANRKKELAALERDIAALERVQ
                     KPFARISYTEAVERLQQAGMPVRWGEDFGGDEETVLANQFDRPVMIHRYPAECKAFYM
                     KTDPDDPKCALCVDVLAPEGYGEIIGGGQREDDYDTLLQKIHTHGLSEEAFGWYLDLR
                     RYGTVPHAGFGLGVERTLGWICGLPHVRETIPFPRLMQRVTP"
     gene            complement(2267..3064)
                     /locus_tag="P5179_06345"
     CDS             complement(2267..3064)
                     /locus_tag="P5179_06345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS94872.1"
                     /translation="MAQHIFAYHAVEDGFELSATTLPLRRWREHCRVLSDLGWRAVRV
                     DEMIQGADDGTFCITVDDAYASLLDVVATCECHGWRGTVFVPTGYVGKVASWDVGAWR
                     THRHLNWEELREVAAAGWEIGVHGHTHTALTDKPIVAARQELQCGLAAVEQELGVVAT
                     SLAYPFGAASKALAEMAQAIGFRRAVTMEPSQVEEWHSPLLLPRWPVYRMDTAAHLQA
                     RLRGAPWLKELERARLRTIQRFSQGTRVRMSGKRNIISDAAEQTYVE"
     gene            complement(3074..3526)
                     /gene="dut"
                     /locus_tag="P5179_06350"
     CDS             complement(3074..3526)
                     /gene="dut"
                     /locus_tag="P5179_06350"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRS94873.1"
                     /translation="MVDRLPIRIVRLPHGKRFPLPAYQTDGAVGMDLVAAIESPLTVP
                     SGHTVRVPSGIAIAIPEGYEGQVRPRSGLASRLPLIIPNSPGTIDSDYRGEVQILLTN
                     IGREAIEISPGTRIAQLVIAPVVRAEWCEVEELPPTGRASGGFGHTGT"
     gene            complement(3528..5681)
                     /locus_tag="P5179_06355"
     CDS             complement(3528..5681)
                     /locus_tag="P5179_06355"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRS94874.1"
                     /translation="MVREYSVAVGDRTITVEHGRMAKQADGAVLVRCGDSMVLVTACM
                     SPQEMDLGFFPLTVEYREKGYAAGKIPGSIFKREGRPSDQETLSARLIDHQIRPLFPK
                     SLKNEIQVIVSVLSHDQENDPDIIACIGASLALSISSIPFAGPYATVRVAHDQEGNKI
                     VLPTYAQLEESDADVVVAGNAESVINIEGETHELPEAALLDLMEFAHGHIKDLCAFQQ
                     KIVDEIGKPKVVPVDTPLDERLVARVEELSVARICESLRVADKVAREEAQAAIAAEVN
                     TALAEEFPGQEKGIASVLEKIAKREMRNMILNEGRRIDGRTIDEVREITSEIGVLPRA
                     HGSALFTRGQTQALASVTLGTKMDERLVDSLEGKSFKTYMLDYNFPPFATGEVKRMSG
                     PGRREIGHGFLAERAIEPVIPASESFPYTIRIVSDILESNGSSSMATVCAASLALMDA
                     GVPIKCAVAGVNCGLVSDGDRFAILTDLLGVEDANGDMDLKIAGTEQGITAFQMDIKV
                     GGIPMETMKLALERAREGRLHVLGLMKQAIAEPKPELSRFAPRILVVKVDPSQIGSII
                     GPGGKTVREIQELTGATIEIDDDGTVRVSSLDGESGQAAVRRINGIVRKPVVGEVFDG
                     VVKSILDFGAVVEYLPGKEGLLHISEIANRRLEKVEDEIKVGDPVQVKIIEVDNQTGK
                     VRLSHKRLLPDYDPSKERERPPRSSQSGHSHGRGR"
     gene            complement(5921..6190)
                     /gene="rpsO"
                     /locus_tag="P5179_06360"
     CDS             complement(5921..6190)
                     /gene="rpsO"
                     /locus_tag="P5179_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRS94875.1"
                     /translation="MPVSKERVAEVMAQHRHHEADTGSSEVQIALLTERIRNLTEHFK
                     THEKDHAGRRGLLKLVGQRRRLLNYLERTNIESYRKLIAELDLRR"
     gene            complement(6303..7202)
                     /gene="truB"
                     /locus_tag="P5179_06365"
     CDS             complement(6303..7202)
                     /gene="truB"
                     /locus_tag="P5179_06365"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRS94876.1"
                     /translation="MCSERALDGIVLLDKPAGGTSHGAVAHLRRILRTRHIGHGGTLD
                     PAATGVLVILVGRATRLAGFLGGGTKVYEATFRFGTATTTDDAAGEVIETADVPELDF
                     GGIEASKNSFVGEIDQVPPAFSAVRSDGVRAYRRARRGQAMELAPRKVTISEIEILEW
                     VRPDLRLKVSCTSGTYIRALARDWGRAIGSAAHVRSLRRVKSGVFDIAECLSFEQIEQ
                     LAASGAWEHVIVPPGPALAKAIGRSTIVAGQDEKRFLNGAPIAHFDDFDGISVVFSRE
                     RELLGIGRVCAGVLRPVLVYPSL"
     gene            complement(7195..7581)
                     /gene="rbfA"
                     /locus_tag="P5179_06370"
     CDS             complement(7195..7581)
                     /gene="rbfA"
                     /locus_tag="P5179_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRS94877.1"
                     /translation="MPFRAEQVADQIRRELAEIIRLRVSDPRVGPITITRVKVSDDLS
                     FARVFFAELTVEGRREGDTLKGLASATGFVRTELGKRIGLRHVPELRFQIDHSIEDGI
                     HLSSLIEKVRTEDKRKLPLEAPGSDV"
     gene            complement(7608..7889)
                     /locus_tag="P5179_06375"
     CDS             complement(7608..7889)
                     /locus_tag="P5179_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRS94878.1"
                     /translation="MVVASVCIGCLVRDSSSLKDKRQVIRSLKDRIRSHFNVSVAEVD
                     HQDLWQRATIGVSVVAVDAQSARHVLEQVIRFVEHDVRLSLLDAPVVIH"
     gene            complement(7902..>9410)
                     /gene="infB"
                     /locus_tag="P5179_06380"
     CDS             complement(7902..>9410)
                     /gene="infB"
                     /locus_tag="P5179_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRS94879.1"
                     /translation="VVTVMGHVDHGKTSLLDYIRRTNVIAGESGGITQHIGAYEVETP
                     QGRVTFLDTPGHEAFTAMRSRGAQVTDIVVLVVAADDNVMPQTIEAINHARAAQVPII
                     VAINKIDLPTANPARIKQQLTQYGVILEDFGGQVQSVEISAKFGQGVDTLLERLALET
                     ELMELKANPNRPARGVVIEARLDKGRGPVATVLVQNGTLRVGDAFLAGMQAGRVRALL
                     DERGNPIKDAGPSRPVQVLGFDDVPMAGDKFAVYESEREAREIAQYRQQIKREQDYSR
                     IKKVSLTDLKRRISEGELRLLPVIVKGDVDGSVEALSDELARLSNAEVAIQVIHRGVG
                     AISESDVLLASASNAIIIGFHVRPDARARELAAQEQVEIRLYQVIYEAIEEVKASLAG
                     LLDAKITEQVTGTAEIRQVFKVPRVGNVAGCMVRSGVMTRGARVRVMRDGAEAYNGKI
                     GTLRRVKEDVREVPSGYECGISIENFNDIKVGDIIEAYTIIEEARTLESVAG"
BASE COUNT         1831 a         3045 c         2522 g         2012 t
ORIGIN      
        1 cggcacacct gttttgggac gggtcgcggc ggtgcggtcc acgtcgcgaa cgtttcgagc
       61 ctgttcgcga tatgcctcaa cttccttgcg acgggaggcc atcgtaagag caggaacaag
      121 cggatgctca ggtgccagta ccatatacgt ggcaccgaaa agagtgtcgg gacgcgtggt
      181 aaacacatcg atcggatagg tggtctggtt tcccggatgg acggcagtga aggtgatccg
      241 cgctccttcg gacctcccga tccagttggc ctgcatcgtc ttgacgcgtt ccggccaatc
      301 caaacgctcg aggtcccgca aaagccgttc cgcatacgcc gttatccgca gataccactg
      361 cacgagatcc cgttttacga cagcagtgcc gcaccgctca caacctccgg ccacgacctc
      421 ttcgttggca aggccgactc ggcagctcgg gcaccagttg accggagccg tgctccggta
      481 tgccaatccc cgcttgtaca tccggaggaa tatccattgc gtccatcggt aatactccgg
      541 atcacaggta gagatctccc gggaccagtc gtacatggcg cctatgattt tgagctggcg
      601 cttcatattc gcgatgttcc gtgcggtgct tatccgcggg tgaatcttgt gtttgatcgc
      661 gaagttctcc gcgggaagac caaatgcatc ccatcctatg ggatgaagga cctggtagcc
      721 gcggagcctc tggtaccgtg accacacatc gctgagcacg taattgcgcc agtgcccgac
      781 gtgcaaccct tcgccggagg gatacgggaa catgtcaaga cagtagtact tcgggcgtga
      841 ggaatcgtcc tttgcctcgt tgatcttgtt ctcttcccac cacgctctcc actgcggttc
      901 cagcgtgcgg aaatcatagg gaatcatgcg aaatgccttc tccagattcg ctctttttgg
      961 ttcttcgttg tgtcagggcg taactcgttg catcaaccgc gggaatggta tcgtttccct
     1021 cacgtgcggc aatccgcaga tccaaccgag tgttcgttcc acacccagcc cgaatcctgc
     1081 gtgcggcacc gttccgtagc ggcgcaaatc gagataccag ccaaatgctt cttcggaaag
     1141 cccgtgagtg tggattttct gcaggagcgt atcatagtca tcttcgcgct gcccgccacc
     1201 gataatctcc ccgtaccctt ccggcgccag tacgtcgaca caaagagcgc acttcgggtc
     1261 atccggatcc gttttcatgt aaaaggcttt gcactcggcg gggtaacggt ggatcatcac
     1321 cggccggtcg aactggttcg caagcacggt ctcctcatcg ccgccgaagt cttcccccca
     1381 gcgcaccggc atccccgcct gttgcaggcg ttctacagcc tccgtgtaac tgattcgggc
     1441 aaagggcttc tgcacccgct ccagcgcggc gatatcgcgt tccagcgcgg cgagttcctt
     1501 cttccggttc gcgacaaccc cttgcacgat ggacaccaga aagtcctctg ccaggtccat
     1561 atcttcgtca agccctatgt gcgccacttc cggttccacc atccagaact cggtgagatg
     1621 ccggcgtgtt tttgacttct ccgcacggaa ggtcggtccg aagcagtaca cgcgcccgaa
     1681 tgcagttgct ccggcctcca tatagagctg cccggactgc gtgaggtacg ccttgctccc
     1741 atcaacgtaa tctgtctcaa aaagagtcgt cgttccttcg caggcagacg gcgtgaatat
     1801 cggggcatcc aacaacagaa aaccatttgc gtcgaaatac gcgcgaatct gcctgatgag
     1861 ttccgccctg atcctcaaaa tcgcctgctg ccgcgaggat ctgagccaca ggtgacggtg
     1921 atccatgagg aacgcagtgc cgtgctcttt cggggaaatc gggtagtcca cttcgggtac
     1981 gtgaatggct tgcagatcga ccacgtccat ctcaaacccg atgggagagc gagcatcggc
     2041 ccgaatctgc cctctcacaa ccagcgagga ctcctgtttg agggccgccg cggtttcgaa
     2101 gacctcaggc gtgacgttcc ctttgaacac cacacactgc atgacgccgg tcccgtcacg
     2161 cacctgaaga aaatgcagct tgcccgacga ccgcatattg ttcaaccacc ccttgaggac
     2221 gatctcctgt ccttcgtacc gggcgatttc ttcgatggtt ggccgctcat tccacatagg
     2281 tctgctccgc ggcgtcagat atgatgtttc tcttccccga catgcgtaca cgcgttccct
     2341 gcgagaatcg ctggatagtc cgcaacctcg cccgctccaa ctctttcaac catggcgctc
     2401 cgcgcaaccg tgcctggagg tgtgctgcgg tgtccatgcg gtacaccggc catcgcggca
     2461 ggagcaacgg ggaatgccat tcttcaactt gagacggctc catggtcacc gcgcgccgaa
     2521 aaccaatcgc ctgcgccatc tccgccagcg cctttgatgc cgcgccaaac ggataggcca
     2581 ggctcgttgc cactacgccc agttcctgct cgactgctgc gagaccgcat tgcagttcct
     2641 gcctcgcggc gacaatcggc ttgtcagtaa gcgccgtatg ggtgtgaccg tgtacgccaa
     2701 tctcccaccc ggctgccgct acttcccgca gttcttccca gttgagatgc ctgtgcgtgc
     2761 gccacgcgcc aacatcccac gacgcgactt ttcctacgta gccggtcggc acaaacacgg
     2821 tcccacgcca accatggcat tcgcacgtcg cgaccacgtc caaaaggctc gcatacgcat
     2881 cgtccactgt gatgcagaac gtgccgtcat ccgctccctg gatcatttca tccacccgaa
     2941 cggcacgcca accaagatcc gacagaaccc tgcaatgttc gcgccaccgt cgcagaggaa
     3001 gcgtcgtggc gctcagttcg aacccatctt ccacggcgtg gtacgcgaag atgtgctgcg
     3061 ccatcgagac cacctacgtg cccgtgtgcc cgaatccacc ggaggcacgt ccggtcggcg
     3121 gaagttcctc gacttcacac cattccgcgc gaacaaccgg cgcaattacc agctgggcaa
     3181 tacgggttcc aggtgagatc tctattgcct cccggccgat gttcgtgagg aggatttgca
     3241 cctctccgcg atagtcgctg tcaattgtcc ccgggctgtt gggaatgatc aacggcaaac
     3301 ggcttgccaa cccgcttctc ggacgaacct gaccctcgta cccttccgga atggcaatcg
     3361 caattccgga aggcaccctg acggtgtgcc ctgaagggac cgtcaatgga gactctatag
     3421 ctgccaccag gtccatccca actgcgccat ccgtctggta cgccggcaga ggaaacctct
     3481 ttccgtgtgg aagacggacg atgcgaatcg gaagccgatc aaccattcta gcgcccgcgc
     3541 ccgtgggaat gccccgattg cgaactccgt ggcggccgtt cgcgttcctt tgacggatcg
     3601 taatcgggca gcaatcgttt gtggctcagg cgaaccttcc ccgtctgatt gtcaacctcg
     3661 atgattttca cctgcacggg atcgccgact ttgatttcgt cttccacttt ctcgagccga
     3721 cggttggcga tttcggagat gtgcagcagg ccttctttgc cgggcagata ctcgacgacc
     3781 gcgccgaaat cgagaatgct cttgacgacg ccgtcaaaaa cctcgccaac caccggtttc
     3841 cgcacaatcc cgttgatcct tcggactgcc gcctggccgc tttccccgtc gagggaggag
     3901 acccgcacag tgccgtcatc atcgatttcg atggtcgcgc cggtgagctc ctggatctca
     3961 cgaaccgttt tcccaccggg gccaatgatc gaacctattt gcgaaggatc cactttcacc
     4021 acgaggatgc gcggagcaaa ccgggagagt tcgggtttgg gttccgcaat cgcctgcttc
     4081 atgaggccca gcacatgcag acgcccttct cttgcgcgct ccagtgccag tttcatggtt
     4141 tccatgggaa ttccaccaac cttgatatcc atctggaagg cggtgatgcc ctgctccgtc
     4201 cccgcgatct tcaggtccat gtcgccgttc gcgtcttcaa ctccgagcag gtcggtcaga
     4261 attgcaaaac ggtcgccatc tgaaaccagc ccgcagttca ccccggcaac tgcgcatttg
     4321 atcgggacac ctgcgtccat cagcgccaga ctcgcggcac agaccgtcgc catggaagag
     4381 gacccgttcg attcgagaat atcactgacg atacgaatcg tgtacgggaa actctcactc
     4441 gcaggaataa caggctcgat ggctcgttcg gcgaggaatc catgccctat ttcgcgtctt
     4501 cccggcccgg acatgcgttt cacttcgccg gttgcgaacg gcgggaaatt gtaatccagc
     4561 atgtacgttt tgaaggattt cccttcaagg gaatcgacca gccgttcgtc catcttcgtc
     4621 ccgagcgtta cggacgcgag agcctgcgtc tgaccgcgag tgaacaaagc ggaaccgtga
     4681 gcacgcggca ggacgccgat ttctgaagtg atctccctga cctcgtcgat ggtgcggccg
     4741 tcgattcgcc gaccttcgtt caagatcata ttccgcattt cccgtttggc aatcttctcc
     4801 aggaccgatg cgattccctt ttcctgaccc ggaaattcct ccgcgagcgc agtgttgact
     4861 tccgcagcga tcgcagcctg cgcctcttcc cgcgcaactt tgtcggcgac cctcaggctt
     4921 tcacagatgc gcgctacgga gagctcttcc acccgcgcca ccagccgttc gtccagaggt
     4981 gtatccacgg ggacgacttt cggtttgccg atctcatcca cgatcttctg ctggaatgcg
     5041 caaagatcct tgatgtggcc gtgggcgaac tccatcaggt cgagcaacgc tgcttcgggc
     5101 aattcgtgcg tctcgccttc gatattgatg accgattccg cattgcccgc gacgacgaca
     5161 tctgcgtccg actcctccaa ctgcgcatag gtgggaagaa cgattttgtt gccttcctgg
     5221 tcgtgcgcta cgcgaaccgt tgcgtacgga cccgcaaacg ggatggagga gatgctgaga
     5281 gccagagacg cgccgatgca ggcgataatg tcggggtcat tttcctgatc gtgagaaagc
     5341 accgacacga tcacctgtat ctcgtttttc aggctcttcg gaaacagcgg ccgaatctgg
     5401 tggtcaatga gccgcgcgga cagcgtctcc tgatcggacg gccttccttc acgtttgaag
     5461 atgctccccg ggattttccc cgcggcgtac cctttctcgc ggtattccac cgtcaaaggg
     5521 aaaaaaccaa gatccatctc ctgcggagac atgcaagcgg ttaccaggac catggagtcg
     5581 ccgcaccgga caaggacagc cccgtccgcc tgtttcgcca tacgcccgtg ttccacggtt
     5641 atcgtgcgat ctccgactgc aacactgtac tcacggacca ttcaggtcct ccttcgtggg
     5701 aacgcgtgaa ttcaacctgt gaccgacgcc aggctcaggg cgtaagcgcg ttctggtctt
     5761 tgccgtaccc atgcgggtga cggtgacgcg cctgcgcata catcgaagcc ttgaacgtga
     5821 tcccggttcg ggacacgtcg ggagtgacgc cgggccggac atgccggcaa acccctcacc
     5881 ccgcggattc tcacagggcc ctcggggtgg ggtaccatgc ctaacgacgc aaatcgagct
     5941 ctgcgataag cttgcgatag ctctcaatat tcgtccgctc gagataattc aacaatcggc
     6001 ggcgctggcc aacaagcttg agcagcccgc ggcggccggc atggtctttc tcgtgtgtct
     6061 tgaagtgctc ggtaaggttc cgaatccgct ctgtgagcag cgcgatctgt acttctgacg
     6121 atccggtatc agcctcgtga tggcgatgtt gagccatgac ttctgcgaca cgctctttgg
     6181 aaacaggcac ctgaatctcc tttctcaccg gatgcggagt cttgcggctc cgcaccacac
     6241 agcctccggt gtctggaggc agggcgaaac gtctttccca tcccgctggc ggcctttccg
     6301 ctttacagtg aggggtacac caacaccggc cgcaatacgc cggcacacac tcttcctatg
     6361 cccagtaatt cgcgttcccg cgaaaacaca accgaaattc cgtcaaagtc atcgaagtga
     6421 gcgatcggcg ccccgttgag aaaacgtttc tcatcttgcc ccgcaacgat cgtcgagcgc
     6481 ccgatcgcct tcgccagcgc gggccccggc ggcacgatga catgctccca cgcgcccgac
     6541 gccgccaact gctcaatctg ctcgaaagac aggcattctg cgatatcaaa tacgcctgat
     6601 tttactctcc gcaacgatcg cacatgcgcc gcgcttccga tcgcccttcc ccaatcacgc
     6661 gccaatgcgc ggatgtacgt tccactggtg cacgacacct tcagacggag gtccgggcgt
     6721 acccattcca ggatttcgat ctccgaaatc gtgaccttcc gcggggccag ttccattgcc
     6781 tgtccgcggc gtgcccgccg gtaggctcga accccatccg accgaactgc gctgaaggct
     6841 ggtggcacct ggtcgatttc gccgacaaag ctgttcttcg aggcttcgat acccccaaaa
     6901 tccagttcgg gaacgtccgc ggtctcgatc acctctcccg ccgcgtcgtc tgtcgttgtc
     6961 gcggttccga agcggaacgt cgcttcgtac accttcgttc cgccgcccag gaaccccgcc
     7021 agccgagtcg cgcgcccaac cagaatcacc agcacgcccg ttgccgcggg gtccagcgtg
     7081 cctccgtggc cgatgtgacg ggtgcgcaaa attcttctca gatgcgccac cgcaccgtgc
     7141 gacgtgccgc cggcgggttt gtcgagcagg acaatcccgt ccagagcccg ctcgctacac
     7201 atctgaacct ggcgcctcaa gtggcagttt gcgcttgtcc tccgttcgca ccttttcgat
     7261 cagactgctc agatgtatgc cgtcttcgat ggagtggtct atctgaaacc tcagttcggg
     7321 gacatgccgc aacccaatac gcttcccgag ctccgttcgt acgaacccgg tcgcactggc
     7381 gaggcctttg agagtatcgc cttccctgcg cccttcgacg gtcaactccg cgaaaaagac
     7441 acgggcaaag gaaagatcat cggagacttt caccctcgtg atggtaatcg ggcctacccg
     7501 cgggtcgctc acgcggagac gaatgatctc cgcgagctcc ctccggatct gatcggcaac
     7561 ctgttccgcc cgaaaaggca tggtaccccg ttcataagac ctgatgatca gtggatgacc
     7621 accggcgcat ccaacaacga taaccgcacg tcgtgttcca cgaaccgtat cacctgttcg
     7681 aggacatggc gcgcggactg ggcatcaacg gcgacgaccg aaaccccgat tgttgcccgc
     7741 tgccacaggt cctggtggtc aacttccgca accgagacat tgaaatggga ccgtattcga
     7801 tccttgagac tcctgattac ctgacgtttg tccttgagcg agctgctgtc gcgaaccaga
     7861 cacccgatac atacactggc gaccaccatc ggtttcaccg atcatcctgc gacggactcc
     7921 agggtgcgag cttcttctat tatggtgtac gcttcaataa tgtcgcccac tttgatgtcg
     7981 ttgaagttct caatcgagat accacattcg tacccggaag gaacctcccg aacatcctct
     8041 tttacgcgcc ggagtgtccc gattttaccg ttgtacgcct ccgcaccatc ccgcatgacc
     8101 cgcacacgtg cgccgcgcgt catgacaccg ctccggacca tacaacccgc aacgttgccc
     8161 acacgcggaa ccttgaagac ctgcctgatc tctgcggtcc cggtaacctg ctcggtgatc
     8221 ttggcatcca gaaggcccgc gagggaggct ttgacctctt caattgcctc gtagatgacc
     8281 tgataaagac gtatttccac ctgctcctga gcggcaagtt ctctggcgcg cgcatccggg
     8341 cgaacgtgga acccgatgat gatcgcgttc gaggcagaag caagcagcac gtcgctttca
     8401 ctgatcgcgc cgacgccgcg gtggattacc tgaatcgcca cttcagcgtt ggacaaccgt
     8461 gccagttcgt cactcagcgc ttccacggaa ccgtccacgt cgcctttgac gatgaccggc
     8521 aggagccgca gctcgccttc gctgatccgt cgcttgaggt cagtgagtga gactttcttg
     8581 atccggctgt agtcctgctc ccgcttgatc tgctgccggt actgtgcgat ttcacgagct
     8641 tcgcgttcgc tctcgtagac cgcaaacttg tctcccgcca tgggaacgtc atcaaagccc
     8701 agaacctgca ccggcctgct gggacccgca tctttgatcg ggtttccgcg ctcgtcgagc
     8761 agtgcgcgca cccggcccgc ctgcattcct gcaaggaagg catctcctac gcgcaacgtc
     8821 ccattctgaa cgagtacggt ggcaacaggc ccacgtccct tgtcaagccg cgcttcgatc
     8881 acaacaccgc gggcaggcct gttgggattc gctttcaatt ccatgagttc ggtttcgagc
     8941 gcgagacgtt ccagcagtgt atcaacccct tgcccgaact ttgcggaaat ctccaccgac
     9001 tggacctgcc caccgaagtc ctccagaatg acaccgtact gcgtgagctg ctgtttgatg
     9061 cgcgccgggt tggccgtcgg aaggtcgatc ttgttgatgg ctacaatgat gggaacctgc
     9121 gccgcacgtg cgtggttgat tgcctcgatt gtctgaggca tcacattgtc atccgcggcg
     9181 acaacaagaa ccacgatatc agtcacctgt gcaccgcgtg agcgcatggc ggtgaacgct
     9241 tcatgacccg gtgtgtcaag gaatgtcact cgtccttggg gcgtttcgac ttcgtacgcg
     9301 ccgatatgct gggtgatccc cccggactcg ccggcgatga cgttcgtccg gcggatgtag
     9361 tccagaagcg aagtctttcc gtggtccaca tggcccataa ccgtgacgac
//
LOCUS       DAOTNG010000100         9406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6717_length_9406_cov_4.986739, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000100 DAOTNG010000000
VERSION     DAOTNG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6717_length_9406_cov_4.986739"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="P5179_06385"
     CDS             complement(<1..620)
                     /locus_tag="P5179_06385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957126.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRS94880.1"
                     /translation="MKPVRHLVSMSDFSEEEINGLIDLARKLRVEWRSGIPRHTLASK
                     TLAMVFEKPSLRTRVTFESGMTQLGGHAIYLAPGDIQIGKREPVADVARNLSRWVQGI
                     MARTYSHDTVAGLAEHAAVPVINALSDLEHPCQALALAMAIRDRRELRGTHVVFVGDG
                     NNVAHSIMLLCAITGMRFTISCPAGYEPSEVIREKCRELRGTCAIV"
     gene            complement(630..1991)
                     /gene="hslU"
                     /locus_tag="P5179_06390"
     CDS             complement(630..1991)
                     /gene="hslU"
                     /locus_tag="P5179_06390"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRS94881.1"
                     /translation="MPVPAVAGAFTPAQVVAYLDRFIVGQQKAKRAVAIALRNRWRRQ
                     RVEGNLRNEITPNNILMIGPTGVGKTEIARRLAALAGAPFVKVEASKFTEVGYVGRDV
                     ESMVRDLAEAAIGLVRSERLESVREPARRAAQDRLLDVLLPPGSGDEQPTAETDDGAR
                     ERVRRRLRAKLEAGEFDERIVELDLPQNRMPLLEVFTPQGGMEEMGMNLGSVFGNMFP
                     GRTKRRKVKVREALRILEQEEAGKLIDSEDVTNEAIRRAEELGIIFIDELDKVAGREG
                     KGGPDVSREGVQRDLLPVVEGTTVSTKHGAVRTDHVLFIAAGAFHVAKPGDLIPELQG
                     RFPIRVELDSLGADDFVRILTEPQNALVKQYTALLETEGVRVEFVPEAIRKIAEVANE
                     VNSRSENIGARRLHTVMTVLLDDILFAVPDEQIREVVITPERVETRLRSILQDDNLSR
                     YVL"
     gene            2196..3020
                     /locus_tag="P5179_06395"
     CDS             2196..3020
                     /locus_tag="P5179_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRS94882.1"
                     /translation="MAIRLVAIDIDRTLLNDRRELSPANERALRDLVERGISVALCSG
                     RDLPSTQAISAPLGLPLWLVVQNGSLVIDPSGNAAYLCSMEKPTALAVLDILERHGLA
                     PVVYEVYPRAHCLWWQTGARAAPGMIEFRTQHGAQVSHVEDIRSVLTPEVSHLEVFDD
                     ADRVVRAGQDFATLDEVVAITNMSSSVVGSALMGLYPRGTAKEEALARVCDVLRISRT
                     EVLAIGDNLNDVGMVRWAGIGVMMANGPEEARLEADWVAPSNNENGVAVAVERFTK"
     gene            complement(3010..4506)
                     /gene="glgA"
                     /locus_tag="P5179_06400"
     CDS             complement(3010..4506)
                     /gene="glgA"
                     /locus_tag="P5179_06400"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRS94883.1"
                     /translation="MHREIRVFMVAAEGVPFAKTGGLADVVGALPYALAEHGCSVRCF
                     LPRYRQVDLEKFGLSTLLPRRTVSFAGAEVAYRVLAAPAERGVEWLFLDAPGFYDRDA
                     LYGTYGEDYPDSCERFAFFCQQALATVRELDWRPDVVHCHDWQSGLIPAYLKLKTRTG
                     IIDTFDTFFSEIRSLFTIHNIGYQGMFSADKLWITGFSPEHFTYDKLEYWGSINFLKA
                     GIVFADRISTVSKRYSEEIQTEAFGRGMDGVLRARAADLTGILNGIDVETWNPATDPH
                     IAANYTAEDLRGKRECRRALSKEVGLNPRVRVPLIGIISRLEEEKGFDLLSSAIPRIM
                     AMGAQMVVLGTGSPEYHTILSRMARRYSGQFAAVLAFDPVLAHRVEAGADMFLMPSKY
                     EPCGLTQMYSMRYGTVPIVHATGGLADTVTNYDGRTKRGNGFSFSRYSVDALLTAVRR
                     AMKFFNDRERWESIMRAGMRQDFSWSAAAEQYRVLYDELLNRADHTTS"
     gene            complement(4520..6253)
                     /locus_tag="P5179_06405"
     CDS             complement(4520..6253)
                     /locus_tag="P5179_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HRS94884.1"
                     /translation="MPETSRSFVLVLHTHLPYVLSHGKWPHGMDWLNEAAAECYLPLL
                     NAFERMVSRGVSPRVTIGITPVLAEQLADAAFKDDFGGYLRMKVDAAKTDEAYFHKIG
                     DTQREELACYWQMVYRQLIDDFENYSRDIIGSFRELQDGGHIEIMTSAATHGYLALLG
                     KDECVRAQVRQGVETYRRHFGRSPRGIWLPECAYRPPYNWTTPVPSEIEPADRLGIDS
                     ILSDNGLEYFVLDTHLLRGGKAIGAYIDRFGALKALWEQFEEAYPDAKKERDLSPLLP
                     YYAGGVLPGQKPVAFFTRHPECSLQVWSGEWGYPGDGAYLDFHKKHFPGGHRYWAVTG
                     RRVDLGDKVLYDPAAVEARLEENADHFVDLVGKALDGQGAGNPALVCAPFDTELFGHW
                     WFEGPRWIEKVLTKMAERADYQPATGSDALKAIPPREILSVPEGSWGEGGYHYIWLND
                     LNKWTWTHVYDAERTYCALVEELATSTLPEVHRVLKQAARELLLLESSDWQFLISTWS
                     ARDYAELRFTHHWETIKRLFGILERLARRERLEESDWLFVEECEAKDGLFPDIEPRWW
                     MPGKRVLPGIS"
     gene            6586..8871
                     /gene="bamA"
                     /locus_tag="P5179_06410"
     CDS             6586..8871
                     /gene="bamA"
                     /locus_tag="P5179_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRS94885.1"
                     /translation="MHYHRILVPGVLLCLCTWSLSRAARVDSLIVRGNVRASETLIRS
                     ASGLSTGAEILIPQIQAAIRNLYATGVFSDVVIKREAIGFNREKLIIEVTEYPVLNNL
                     TFSGNRKLKRDDISKKVRILPGQVVNPQALTRAIADIQKEYRDKGYYLARVVPEQKPA
                     GPGRVDLNLAIREGDRVQVERIRFIGARAFNPRKLRDQMSTRQNRWWRSADFDEEKLR
                     EDKEKILSFYRKFGYREAAIVRDSVYLDDSKKKLFIDLFVDEGPQYHFGRITWAGNLL
                     LSDEQVHRVLRLREGDVYNREAFDEAVFRLSSAYQEQGYWAMDPGIMETPRGDTVDVS
                     FNIVESEPSRVQFVDILGNDKTKDKVIRREMTLVPGDIFRRSDLERSNRNVFYLNYFE
                     NVEPNIRPAAGGNVDVTMTVKEKPTGTVNMAVGYGEADKWVGSIGLSIPNLRGNGQQA
                     DFQWEFGQVTTSFYLSFTEPWLFDTPTSGSITLFNTRREYDVTEESRGFSLRAGRRLR
                     WPDDYSRVYASYSLRTEDYTFPLDYTDADKLAYISNPSDPHLISSALGIGYVRDSRNL
                     PLFPTDGTYFSYDLQMAGGPVGGDVSYRKHTIELDYFLPLFEIKGWGPALALKTTFGQ
                     INTAQPLAVPLSERFRPGGVSFDGQIRGYYDYAVGPKSEYGSSTGGYTMLLTTAELSF
                     PVVQQQIYGVLFADAGNAWRGLAEMNPYRLKRSYGFGARFILPLAGVIGLDFAKGVDP
                     DPYSGGGGWETHFQFGPTIMR"
     gene            8913..>9406
                     /locus_tag="P5179_06415"
     CDS             8913..>9406
                     /locus_tag="P5179_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS94886.1"
                     /translation="MRKMRWVVPVVAAVCSLFLGASRAQAQSFKVAVIYMDEIREKGQ
                     PYIEALQRLQQVSQQRQQEAQTKQGEIQRLQEQLQRQASLMTEQRRTQSEAQIRQKVT
                     EYEQWAGQAQQELQQQQIQAIQPIDERVLQIVERIANERGIDVVLDGVAVAFIKNKEQ
                     NNLT"
BASE COUNT         2043 a         2841 c         2540 g         1982 t
ORIGIN      
        1 tctacaatcg cgcacgtgcc gcgcagttcg cggcactttt cccgaataac ctcggatggt
       61 tcataccccg ccgggcaact gatcgtgaaa cgcatccctg taatggcaca taacaacatg
      121 atcgaatgcg ctacattgtt tccgtcaccg acaaagacaa cgtgcgttcc gcgaagctct
      181 ctcctgtctc gtatcgccat ggcaagcgca agtgcctgac aggggtgctc gaggtcgctg
      241 agggcgttga tcacgggaac cgccgcatgt tccgccagtc ccgcgaccgt gtcatgcgag
      301 tacgttctcg ccatgatgcc ctgcacccag cgggaaaggt ttcgcgcgac atcggcgacc
      361 ggttcgcgtt ttccgatctg gatgtcaccc ggcgcgaggt aaatcgcgtg gccaccgagt
      421 tgcgtcattc cactttcaaa cgtcaccctg gttcgcagag atggcttctc aaaaaccatc
      481 gccaacgttt tggaggcgag cgtgtgtcgt ggaatcccag atcgccattc cacccgcagc
      541 ttccgggcca gatcgatcag tccgttgatc tcttcttcgg agaagtcgct catcgagaca
      601 agatgccgta ctggtttcat catcattccc tatagcacat agcggctgag gttatcgtcc
      661 tgcagaatgg aacggagtct tgtttccacc cgttccgggg tgatgactac ctcccgaatc
      721 tgctcgtccg gaaccgcaaa cagaatgtcg tcaagaagaa ccgtcatcac ggtgtggaga
      781 cgccgggcac ctatgttttc cgatcgtgaa ttcacttcgt tcgctacttc cgcgatcttc
      841 cggatcgctt caggcacaaa ctcgaccctc acgccttccg tctccagaag agcagtgtac
      901 tgcttcacga gagcattctg cggttccgtg aggattcgga cgaaatcgtc ggcaccaaga
      961 ctatccagtt cgacacggat cggaaagcgc ccctggagct ccggtatcag atcgcccggc
     1021 ttcgccacat ggaacgcacc ggcagcgatg aaaagaacgt ggtccgtccg cacggcgcca
     1081 tgtttcgtac tgacggtggt gccttccacg acggggagga gatcgcgctg caccccctca
     1141 cgggaaacat ctggacctcc cttcccctca cggcccgcaa ctttgtcgag ttcgtcgatg
     1201 aatattatcc ccagttcttc cgcgcgacga atggcctcgt ttgttacgtc ctcgctgtca
     1261 ataagcttcc cggcttcttc ctgctccagt attctgagcg cttccctcac tttcactttc
     1321 cgccgcttcg ttcgccccgg aaacatgttg ccgaacacac tccccaggtt catccccatc
     1381 tcttccatac cgccctgcgg ggtaaaaacc tcaaggagcg gcattctgtt ctgcggcaaa
     1441 tccagttcca ctatccgctc gtcgaactca cccgcttcaa gtttggctcg gagccgccgt
     1501 cgaacccgtt cccgcgctcc gtcgtcagtt tcggcggtgg gttgttcatc ccctgacccc
     1561 ggcggaagca aaacatccag caggcggtct tgtgcggctc tgcgggctgg ttctcgaacc
     1621 gattccagtc gttccgaacg cacgaggcca attgcagcct cggcaaggtc gcggaccatg
     1681 gattcaacat cccgcccgac atacccgacc tcggtgaatt tcgaggcctc gacttttacg
     1741 aacggtgcac cggcaagcgc cgcgagacgc ctcgcgattt ccgttttgcc aacccctgtg
     1801 gggccaatca tcagaatgtt gttcggcgtt atttcgttgc gaagattccc ttccacgcgt
     1861 tgacgtcgcc atctgtttcg taacgcgatc gccaccgccc tttttgcttt ttgctgcccc
     1921 acaatgaatc gatcgagata cgcaacaacc tgcgccggtg tgaacgctcc agcaactgca
     1981 ggaacaggca tatcagcaat cgcgtctccc gcgaattcca acgcaaatcc cctctcttcc
     2041 gagcacactc caatcgcaac ccgagtacag cacgacaaga agatacctaa ctgcggtacg
     2101 ccgggaaacg cccgtgggtt gacacgctac tgcatgtacc gctcattcag agtccgtttg
     2161 agcaacttga cgcgatccaa ttcccaggtg aatgcatggc tatcagactt gttgcgatag
     2221 atatcgaccg tactcttctg aacgaccgga gagagctttc acccgcgaac gagagggccc
     2281 tccgggatct cgtggagcgc gggatttccg tggcgttgtg cagcggaagg gacctcccct
     2341 caacgcaagc catatctgct ccactcggtt tgccgctgtg gttggtcgtt caaaacgggt
     2401 cgctcgtaat cgatccgtcg gggaacgcgg cctatctttg ttccatggaa aagccgacgg
     2461 cgctcgccgt gcttgatatc ctcgaacgcc acgggcttgc accggtggtg tacgaggtgt
     2521 acccgcgtgc gcattgtctg tggtggcaga ccggggctcg agcggctccg gggatgattg
     2581 aatttcgcac gcaacacggc gcccaggtat cgcatgttga agacattcgt tcagttctta
     2641 cgccggaggt ttctcacctc gaagtcttcg atgacgcaga ccgtgttgtg cgtgcgggtc
     2701 aggatttcgc cacgttggat gaagtggtgg cgatcacgaa catgagttcc tcggttgtcg
     2761 gcagcgccct gatggggttg tacccgcgtg gaactgccaa agaggaagct ttggcgcgcg
     2821 tctgcgatgt tcttcggatt tcgcgaactg aggtcctcgc aatcggtgac aatctcaacg
     2881 atgttggcat ggtccggtgg gcggggattg gcgtgatgat ggcaaacggc cccgaagaag
     2941 cgcgcctcga ggccgattgg gtagccccct caaataacga aaatggggtt gcggttgccg
     3001 tggaacggtt cacgaagtag tatgatccgc gcggttcaac agctcgtcat atagcacgcg
     3061 gtattgctca gcggccgcag accaggaaaa atcctggcgc atgcccgccc gcatgatcga
     3121 ctcccacctc tcgcggtcgt taaaaaactt catggcgcgg cgcacagcgg tcaggagcgc
     3181 gtccaccgag taacgcgaga aagaaaagcc gttccctcgc tttgttcgtc catcatagtt
     3241 cgtaaccgta tctgcaaggc cgccggtggc gtgcacgatc ggtaccgtgc cgtaccgcat
     3301 gctgtacatc tgggtgagcc cgcacggttc atatttcgag ggcatcaaaa acatatcggc
     3361 acccgcctcg acccgatgtg cgagcactgg gtcgaaggca agcaccgcgg caaactgccc
     3421 cgaatagcgt cgcgccattc gagaaagtat cgtgtgatac tccggagaac ccgtgcccag
     3481 aacaaccatt tgcgcgccca tcgccattat gcgggggatg gcagaggaaa gaagatcgaa
     3541 gcccttttcc tcctcgagcc ggctgatgat gccaatgagc ggaacacgaa cgcgggggtt
     3601 cagtcccact tctttcgaca gcgcgcgccg acattcccgc tttccgcgca gatcctccgc
     3661 agtgtaattc gccgcgatgt gtggatcagt cgccggattc cacgtttcta cgtcgatgcc
     3721 gttcaggatt cccgtcaaat cggctgctcg cgcgcgcaac acgccatcca tacctctgcc
     3781 gaaggcttca gtctggattt cctcgctgta gcgcttgctc accgtggaga tgcggtccgc
     3841 aaaaacgatc ccggccttga ggaagttgat ggagccccag tattcgagtt tgtcatacgt
     3901 gaaatgctcc ggcgaaaacc cggtaatcca cagcttgtcc gcgctgaaca tcccttgata
     3961 gccgatattg tgaatcgtga aaaggcttct gatttccgaa aagaacgtgt cgaaagtgtc
     4021 gataataccg gtacgcgtct tcagtttgag atacgcagga atcagccctg attgccaatc
     4081 gtggcagtgc acaacgtcag gacgccagtc cagttcgcga acagtcgcca gcgcctgctg
     4141 acagaaaaac gcgaagcgtt cgcacgaatc cgggtagtct tcaccgtagg taccgtataa
     4201 cgcgtcccgg tcgtaaaaac ccggcgcgtc cagaaacaac cactccacgc ccctctctgc
     4261 aggagccgcg agaacgcggt acgcaacttc cgcccccgca aaggaaactg tgcgccgtgg
     4321 cagaagcgta ctaagaccga acttctcgag atctacctgg cggtagcgcg gcaggaagca
     4381 acgaacgctg cacccgtgct cagcaagggc gtagggcagg gcaccaacaa cgtctgcaag
     4441 cccccccgtc ttcgcgaacg gaactccctc cgctgcgacc atgaacaccc ttatttcgcg
     4501 atgcatacgc atcctcccct caactgatgc cgggcaacac ccgttttccc ggcatccacc
     4561 atctgggctc tatgtcgggg aaaagaccgt cttttgcctc acattcctca acaaacaacc
     4621 agtcactctc ctccagacgc tcacgccgcg ccagacgttc cagtatcccg aaaaggcgtt
     4681 tgattgtctc ccagtggtgg gtgaacctca gttccgcgta atcccgggcg gaccatgtac
     4741 tgatcaggaa ttgccagtcc gatgactcca acaggagaag ctcgcgcgcc gcctgtttca
     4801 gcacacggtg tacctccggc agagtactcg ttgccagctc ctcaacaagt gcgcaatatg
     4861 ttcgttccgc atcgtatacg tgggtccacg tccacttgtt caaatcgttc agccagatgt
     4921 agtggtaccc accctcaccc caggaaccct ccggcacgct cagaatttcc cgcggaggaa
     4981 tcgccttcag ggcatcgctg cccgtcgcgg gttgataatc ggcacgctcc gccatcttgg
     5041 tcagcacttt ctcgatccag cgcggccctt cgaaccacca gtgtccgaac aattcggtgt
     5101 cgaaaggagc gcagacaagc gcgggattcc ccgcaccctg gccgtcaagc gcttttccaa
     5161 caaggtccac aaaatgatcg gcattctctt ccagtctggc ctctaccgca gccgggtcgt
     5221 aaagaacctt gtcgccaagg tcaacacggc ggcccgtcac tgcccagtag cggtgcccgc
     5281 cgggaaagtg ctttttgtgg aaatccaggt atgccccgtc tcccgggtat ccccattctc
     5341 cggaccacac ctgaaggctg cactccggat gacgcgtgaa gaaggccaca ggtttttgcc
     5401 cgggcaggac ccctcccgcg tagtacggca gcaacgggct gaggtcgcgc tccttttttg
     5461 cgtccggata cgcttcttcg aactgttccc agagggcttt caacgcgccg aagcggtcaa
     5521 tgtaggctcc gatagctttg ccgccccgca gaagatgtgt atccagcacg aagtattcga
     5581 gaccgttgtc gctcagaatt gaatctatcc cgagtctatc cgcgggttct atctccgacg
     5641 gcacaggcgt ggtccagttg tacggcgggc gatacgcgca ttccgggagc cagattcctc
     5701 tgggtgatcg cccgaagtga cggcggtacg tttccactcc ctgccggacc tgcgcccgaa
     5761 cacattcgtc tttcccaaga agtgcgagat atccgtgcgt ggcggcactc gtcatgattt
     5821 ctatgtgccc gccgtcctgc aactcccgga acgacccgat gatgtctcgt gaatagttct
     5881 cgaaatcatc gatgagctgc cggtacacca tctgccagta gcaggccagt tcctcacgct
     5941 gcgtgtcgcc aattttgtgg aaatatgcct cgtcagtttt cgcagcatcc actttcatcc
     6001 ggaggtaccc gccgaaatca tccttgaacg ccgcgtcggc cagctgttcc gcaagaaccg
     6061 gcgtaatccc gattgtcaca cgcggcgaaa cacctcgcga caccattcgt tcaaacgcgt
     6121 tcaacaaagg caggtagcac tcggcggcgg cctcattcaa ccaatccatc ccgtggggcc
     6181 acttgccgtg agaaaggacg taaggcaggt gcgtatgaag cacgagaacg aaggaacggc
     6241 tggtttcagg catgaaaact ctccggttca gatgcgcaga aatggaaaac cacgaagcac
     6301 ttttcctggc gaacgtcata gacgtgttca caatcttgca cggccgtacc atataatacg
     6361 caaatgactc caacgcgcag tgttgcatgc gctgtgtcga tggaactgag ttcgaaccct
     6421 ccgcgtcaca tgtgcatgcc gtctcgactc tccaaccgct tcttcggcac caaccactgt
     6481 gcaggaaact cgtcgcactg tatttttagt ctttcccggg cgagacagaa gcctgtaaat
     6541 cgatgcgatc gaacacccga atccctcccg tgaggtccgc gaatcgtgca ttatcaccga
     6601 atcttggtac cgggggtttt gctgtgtctg tgcacgtgga gcctttcccg tgcggcacgc
     6661 gttgatagcc tgattgttcg tggcaatgtc cgggcatccg aaacactcat tcgttccgca
     6721 tccgggcttt ccaccggcgc ggaaatcctc atcccgcaga tccaggcggc catcaggaac
     6781 ctgtacgcaa cgggcgtgtt cagcgatgtc gtgatcaaac gagaagcgat cggattcaat
     6841 cgcgagaagc tgatcattga ggttaccgaa taccctgttc tcaacaacct tacgttttcg
     6901 ggaaaccgaa aactcaaacg agatgacata tccaagaaag tgcgtattct tccaggtcag
     6961 gtggtaaatc cacaggccct cacccgcgcc atcgccgata tccaaaaaga gtaccgagac
     7021 aaggggtact acctcgcgcg ggtcgtcccg gaacagaaac ctgccgggcc cggaagggtg
     7081 gacctgaacc tcgcgattcg agaaggcgac cgcgtgcagg tggagcggat tcgtttcatc
     7141 ggcgcacgcg ccttcaatcc gcggaaactg cgcgatcaga tgagcacgcg acagaaccgc
     7201 tggtggaggt cggctgactt cgacgaggaa aaacttcggg aagacaaaga gaaaattctg
     7261 tccttctatc gcaagttcgg gtaccgcgaa gcggcgattg ttcgtgattc tgtctacctc
     7321 gatgactcca agaaaaagct gttcatcgat ctgttcgttg acgaaggccc gcagtatcat
     7381 ttcggccgca tcacctgggc gggcaacctg ttgttgtccg acgagcaggt gcatcgtgtt
     7441 cttcgcctcc gtgaggggga cgtctacaac cgggaagcgt tcgacgaagc agtgtttcgt
     7501 ctgtcgtccg cgtaccagga acaaggctac tgggcaatgg atcccggaat catggaaacc
     7561 ccgcgcggag atacggtcga cgtctcgttc aacatcgtgg agtccgaacc ctcccgggtt
     7621 cagttcgtcg acattctggg gaacgacaag acaaaggaca aagtcatccg gcgtgaaatg
     7681 accctcgtgc cgggcgacat tttccgtcgt tcggatctcg aacggagcaa tcggaacgtc
     7741 ttctacctca actacttcga aaacgtcgag cccaatatca gaccggccgc tggcggaaac
     7801 gtcgacgtca ccatgacggt gaaggaaaaa ccgacaggaa cggtcaacat ggcagtgggt
     7861 tacggcgagg ccgacaaatg ggttggttca atcggccttt ccatccccaa tctgagagga
     7921 aacggtcaac aggccgattt ccagtgggag ttcggccagg ttaccaccag tttttacctg
     7981 agtttcactg aaccatggct gttcgatact ccgacatcgg gctccataac gctgttcaac
     8041 acccgcaggg agtatgacgt taccgaggag agccgggggt tcagtctgcg tgccggcagg
     8101 cgccttcgtt ggcccgacga ctactcccgc gtgtacgcaa gctattcact ccgcaccgag
     8161 gactacacat tcccgctcga ctacactgac gcggacaaac tcgcgtacat cagcaatcca
     8221 tccgatccgc accttataag ttccgccttg ggaatcggtt acgtgcgaga ttcgcggaat
     8281 ttgcccctct tccccactga tggaacatat ttctcctacg acctgcagat ggcgggcgga
     8341 cctgttggtg gggatgtctc ttatcggaag cacacaatcg aacttgacta ttttctgccg
     8401 ttgttcgaaa tcaagggttg gggccccgcg ttggccctca aaacaacatt cggccaaatc
     8461 aacaccgctc aacccttggc cgtacctctc agcgagcgat tccgtccggg cggggtgagt
     8521 tttgacggac agattcgtgg atattacgac tatgccgtcg ggccgaaaag cgaatatggc
     8581 agcagcaccg gcgggtacac gatgttgctc acaacagcgg agctgagctt ccccgttgtt
     8641 caacaacaga tctacggcgt cctttttgcg gacgctggaa atgcctggcg cggcctggcg
     8701 gagatgaacc cgtaccgact caagcgctca tacggattcg gtgcccgatt cattcttccc
     8761 ctggctggcg tcatcggcct cgacttcgca aaaggggttg acccggatcc ttactccggt
     8821 ggtggcgggt gggaaacaca cttccagttt ggcccgacta tcatgaggtg agcgatttcc
     8881 gtaaccacga tttccagaag gagatgtttc ctatgcgcaa aatgagatgg gtggttcctg
     8941 ttgtagcagc cgtctgcagc cttttcctgg gcgcatctcg cgcacaggcg cagagtttca
     9001 aagtcgcggt catctacatg gatgaaattc gcgagaaggg acaaccctac atcgaagccc
     9061 tgcagcggct tcagcaggtg agccaacagc gccagcagga agcccagacg aagcaggggg
     9121 agatccagcg attgcaggag caactgcaac gtcaggcgag tctgatgacg gagcaacggc
     9181 gtacgcaatc ggaagcgcaa atccggcaga aggtcaccga atacgaacaa tgggccggtc
     9241 aggcgcaaca ggagctccag caacaacaga ttcaggcaat ccaaccgatc gacgagcgcg
     9301 tccttcaaat tgtcgagagg atcgccaacg aacggggtat cgatgtggta ctggacggcg
     9361 ttgcggtggc tttcatcaaa aacaaagaac aaaacaatct cacgaa
//
LOCUS       DAOTNG010000101         9359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6742_length_9359_cov_5.030847, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000101 DAOTNG010000000
VERSION     DAOTNG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_9359_cov_5.030847"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5179_06420"
     CDS             complement(<1..391)
                     /locus_tag="P5179_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRS94887.1"
                     /translation="MTGNLQDGWWVVSIASLAFITWYAVRGWQMGALRTLLWVAGFAF
                     GLFLAARYAGGVSGLLGKTIPGSAREAIAFVVIVLAVMVVVDILNAVLEKFLRSTPLT
                     TLNRAAGALLGILKGVLILALVGVLLAI"
     gene            complement(482..934)
                     /locus_tag="P5179_06425"
     CDS             complement(482..934)
                     /locus_tag="P5179_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRS94888.1"
                     /translation="MTALETRLQEDQKKAMKTGDKSLLGLIRLLRSQLQYARMQAGEE
                     WSEEKAQEVLLAAAKARRESISLFEKGGRMDLVEKEKAELALLERYLPAQLSDQELST
                     LIELCISETGAQDLKDMGKVMAALMQRVRGRADGKVLNERVRARLSGR"
     gene            complement(944..1141)
                     /gene="rpsU"
                     /locus_tag="P5179_06430"
     CDS             complement(944..1141)
                     /gene="rpsU"
                     /locus_tag="P5179_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRS94889.1"
                     /translation="MAGVIVREKEPFEKALKRFKKACEKAGIISDMKKHQHFEKPSER
                     RKRELNAARRKMRKTQILSEK"
     gene            1453..1815
                     /locus_tag="P5179_06435"
     CDS             1453..1815
                     /locus_tag="P5179_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94890.1"
                     /translation="MRKFGEIVVDEGFATEEQINSALTYQKTSDILVGKILVDMRVLT
                     HADVERVSNFLKTPECQGKRFGEAAVFLGACTEADVARAIEIQRTSKGMLGDLLVALG
                     YITERQRQDIVRIQMEEA"
     gene            1883..2917
                     /locus_tag="P5179_06440"
     CDS             1883..2917
                     /locus_tag="P5179_06440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS94891.1"
                     /translation="MRQIHVSSLTSSRRIRVADDKGYFPVLVRAADNALMAVFRDGAA
                     HMGAGGYLVASRSTDLGLTWTEPVVVVNTREYDDRNPAVGVARNGVITVAFHANGSYG
                     PSGDYIKDRRTPRSLHTGIVQSADNGRTWGDPMLWTDATPWDGMSPYGRMLTLADGTM
                     VMSIYNGGSYLLRSRDNGQTWGGLTLTGTDTNESSYCVLPTGEWLCAGRKQRESEDQR
                     ILVRRSSDGGMTWSEPTPFGVNKTLPADLLALSNGSVLLVYGYRTPPFGVRARMSHDN
                     GRTWSETELVVHGSALSADCGYPSALVADGYVIIVFYDAGRTTHDAPDGTGAFCELVR
                     IAESELISVF"
     gene            2929..>3272
                     /locus_tag="P5179_06445"
     CDS             2929..>3272
                     /locus_tag="P5179_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94892.1"
                     /translation="MILRRSVLVFALLWAGYVPAETITTQSGLFRVHYAPGAKETAKQ
                     TAIIAEDVFYQLVTAYSLHERFRPIDILVTDDIDAGNGFADYYQNQLVVWATNLDTDL
                     RGTNQWLRNVVT"
     assembly_gap    3273..3372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3373..>6391
                     /locus_tag="P5179_06450"
     CDS             <3373..>6391
                     /locus_tag="P5179_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94893.1"
                     /translation="DEMGVFAHNWLKSEMVYNQGYALTSYISRNFGANAPRNLAKNTG
                     YVTFNTALRENLRLTGQDLYDRWVADIRARYSSVRDSVGAIVEGNALPDEGSYETAPA
                     ISPDGTQIAYLSARGEDFLLTKPRIRHLARNTVLDIDRSVRGNLAWFPDGGRIAYAKF
                     GRGTYLNDLYVYDIARKEETRITSRLRARDPAVSPDGKWIAFVSGEDGGTRLGIVRAD
                     GSEIRWLTNSMRSPGPGPADIGSKTSDLIQFYTPKWSPDGKSLLFSVFRDGDRDIGII
                     GTQGPYFSVRDALSDSAAFPDTLVYPAGSGFRLLVHTEADERDPAWLPDGSGFVFSAD
                     YDIATDPRNQTPEESVRSDGGSRPFGIFNLYLATLPADSTEEISYRRLTNVLGGAFRP
                     AVAPDGRWLAYVGYHANNFSLYILPLAADGQAALRPPFAGDAVPRSYQHIAKSPPASQ
                     LFHVGQTTGMRTLVGWVPVLRFGPSVIGDRFSVNHVGLGLLAGIEDQMGARYYFGSGE
                     ISKNIGKREPASTGVFLYGDQGLRPLLTTESGLSPRLYGYGARQEICAVTERARTTRA
                     SAPGAVLGANNETIPLNPLSRVELSDSVVGWDNYRYLYGGLGIRAGVGSRQTAAFELG
                     WQNYYVDERLDRRIDNYSVFLDNSNPPQDVTALFPWGNYGYPNPGTPLIWDQRPQFSV
                     AYFRDRTASFRWAYAKITPTVDDLVNPTGGRVITASYSFHRVSVMDSLAGTNYDANYN
                     QIDPTRPLYSQVDRHFSANELVLSYEEYIRLPGVLNRTTLALQGVAAYFDRALKYYDP
                     NDEGSNFLEGWAYWPLRYRLGGGGTLRGYPYFSHEGSKMAFFRASYVFPLLQHHRKQF
                     LSFFHDRTYGALFVETGSTWNWTRLKDAWIARDDFLWDLGAELRISGRMFHYMPVVSY
                     VAVARRMKDVPYPFVDYERTAEGAILFRQPDRYRVYIGAALALGGGGGAAPHAEPRFT
                     RTPQVLMLSNPARQPANGAWESPGTTAEKLPLLF"
     assembly_gap    6392..6491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6492..7495)
                     /gene="trpB"
                     /locus_tag="P5179_06455"
     CDS             complement(<6492..7495)
                     /gene="trpB"
                     /locus_tag="P5179_06455"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRS94894.1"
                     /translation="MTNVPASTYSVPDNTGHFGIYGGRFASETLMPLLSDLAREYEAA
                     KADAGFQEEFSGLLRNYVGRPTPISFAKRLSDHIGGARIFLKREDLAHTGAHKINNTI
                     GQVLLARRMNKRRIIAETGAGQHGVATATVSALFGLDCEVYMGAEDMRRQRLNVFRMR
                     LLGAKVTAVHSGAKTLKDAINEAMRDWIARVDDTYYIFGSVLGPHPYPMIVRDFQSVI
                     GKEARAQMQEEIGRLPDVCLACVGGGSNAMGLFHAFLGDDNVRLIGVEAGGRGTRLGD
                     HAARFRGGRLGVLQGSKSYVLQNEDGQIAPTHSISAGLDYASVGPEHSYLRDLGRTEY
                     T"
     gene            complement(7521..>8018)
                     /locus_tag="P5179_06460"
     CDS             complement(7521..>8018)
                     /locus_tag="P5179_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HRS94895.1"
                     /translation="LCPVFNTNRMVCEYTGRFYRDAENRWRDITADECKKARDLAAWK
                     TRVFREWHNVRVTRIESDHVNELPVGKMLHVLATVSLGNLLPADVSVQVYHGRLDSHG
                     EIVEGTATNMSFVSQGSDAVFEGDVPAIGSGQHGFAIRVVPFHPDLSNPFATGLITWS
                     PESLG"
     assembly_gap    8019..8118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8119..9326)
                     /gene="glgP"
                     /locus_tag="P5179_06465"
     CDS             complement(<8119..9326)
                     /gene="glgP"
                     /locus_tag="P5179_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRS94896.1"
                     /translation="MPLQKAVRNDGTQAFVEVSLPGRAVRAAIWKVQVGRVPVYLLDT
                     NIRENSREDRDITDQLYGGNQEMRIKQEILLGVGGKRALAELGIRPTVFHMNEGHSAF
                     LAVERLREMIQEEKKPFAEALEITEASTVFTTHTPVPAGNDRFAPELIDRYLYGYYAA
                     LGLSREQFLALGQENPGNVHSEFCMTALAIRLAAHTNGVSRLHGRVARGMWKHLWPGL
                     PVEEVPIASITNGVHARSWISNDMGWLFNRYLGTRWMERPADQTIWQAISRIPDEELW
                     RTHERRRERLVAFARRRLRSQLERRGATRREIQSAGEVLNPDVLTVGFARRFATYKRA
                     DLLLRDPDRLIALLTNKERPVQIIFAGKAHPADSPGKELIRKIIHFIRLNDELRRHVA
                     FIEDYDINVAR"
BASE COUNT         1859 a         2681 c         2610 g         1909 t
ORIGIN      
        1 gaattgccag caacacacca acaagcgcca agatgagcac gcccttcaaa atgcccagca
       61 atgcccccgc cgctctgttc agggttgtga gcggggtcga gcgcaggaac ttctcaagca
      121 ccgcattgag tatgtcgacg acaaccatca cggcgaggac gatcaccacg aacgcgatgg
      181 cttcgcgcgc agaacccggg attgttttcc ccagaaggcc ggatacgccg ccagcgtacc
      241 tggccgcgag aaagagcccg aacgcgaacc cggcaaccca caacagcgta cgcagcgcgc
      301 ccatttgcca tccgcgtacc gcataccatg taatgaaggc caggctggcg atgcttacca
      361 cccaccagcc atcctggagg ttacccgtca tgcgacatct gcttccttcc gggcatcaaa
      421 caaaaaaccg cgcacccggt gcgggcgcgg ttctggagac gcgatagcag cctcaaatcg
      481 cctacctgcc gctcaatctg gcgcgaacac gttcgttaag gaccttcccg tcggcccgac
      541 ctcgaactct ctgcatcagc gccgccataa ccttccccat atctttgagg tcttgcgcgc
      601 cggtttcact gatgcacaac tcgatcaggg tggatagttc ctggtcggag agctgcgcgg
      661 gaagatatcg ctccagtagc gccagttccg ccttttcctt ctcgacaagg tccatgcggc
      721 cgcccttctc gaacagggaa atcgactccc gtctggcttt cgcggcggcc aatagaactt
      781 cctgtgcctt ttcctccgac cattcctccc cggcctgcat ccgcgcatac tgaagctgtg
      841 aacgaagaag gcgaatcagt ccgaggagac ttttgtcccc ggtcttcatc gccttcttct
      901 gatcttcctg caaccgcgtt tcgagagcgg tcatttctcc gacctatttt tcggaaagaa
      961 tctgtgtctt ccgcatcttg cgccgcgctg cgttcagttc acgtttccgt cgttcgctgg
     1021 gcttttcaaa gtgctggtgc ttcttcatat cggaaatgat accggccttt tcacacgcct
     1081 tcttaaaacg tttcaacgct ttttcaaagg gttctttttc gcgcacgata acacccgcca
     1141 tccggtttcg cctcctttcg ctggggattt tacatggtat gctatacacc gccggcattc
     1201 gccggataga ccaattctag aatatacgac ttcccggctg cgcgtccaat gcgatgctgc
     1261 gagagagtgg gtgcaacgcg agtcttcttc tgttgacgca gaaccttgcc gccgcgatga
     1321 tctttcaggg gtggcacagc cagcgatgac gggtatcttt gtaatgattt cgccctccgt
     1381 tgtgaaggcg gacaccgtaa aggacccggc acacggttgt gactcaggac aaatggcaca
     1441 ggagttgaag gaatgcggaa atttggcgaa atagtcgtcg acgaaggctt cgcgacggag
     1501 gagcagatca attctgcgct gacgtaccag aagacttcgg acattcttgt cggcaagatt
     1561 cttgttgaca tgcgtgtact cacccatgcg gacgtggaac gggtgtcgaa tttcctgaag
     1621 acacccgaat gccagggaaa acgattcggc gaagccgcgg tttttctcgg tgcgtgcaca
     1681 gaagccgacg tggcacgagc gattgagatc caacggacaa gcaaggggat gctcggagac
     1741 cttctcgtcg cactgggata catcaccgaa cggcaacgtc aggatatcgt ccggatccag
     1801 atggaagagg cgtgacacaa gctcacgcag cgctgcggcg agcataccaa ttgccagttg
     1861 cgggcgtgtg gaggcatctc aggtgcgcca gatacatgtt tcttcgttga cgagttcccg
     1921 gcggattcgc gtagcggatg acaaggggta tttcccggta ctcgtccggg ctgccgataa
     1981 cgctctgatg gctgtttttc gtgacggcgc ggctcacatg ggtgcgggtg gttatctggt
     2041 ggcatcgcgc tcgaccgacc tgggtctgac atggacggaa cctgtggtgg tcgtcaacac
     2101 gcgtgaatat gatgaccgga acccggcagt cggggttgcg cggaacggcg tgatcacggt
     2161 ggcgttccac gcaaacggaa gctacgggcc ctccggcgat tacatcaagg accgccggac
     2221 tccccgctct ctccacaccg gtatcgtgca atctgcggac aacggccgca cgtggggaga
     2281 tcctatgctg tggaccgacg ccacgccatg ggatggtatg tctccctacg gtcgaatgct
     2341 gacgctggct gacggcacga tggtcatgtc catttacaac ggcgggagtt atctgcttcg
     2401 gtcgcgcgat aacggacaga cgtggggcgg actcacgctg acaggcaccg acaccaatga
     2461 gagttcgtac tgcgtgcttc caacgggtga gtggctctgc gcaggacgaa agcaacgcga
     2521 gtctgaagac cagcgcattc ttgtccggcg atcctccgac ggtggcatga cgtggtccga
     2581 acccacaccg ttcggagtca acaagacgtt gccggccgac ctgctggcgc tttcgaacgg
     2641 atctgttctc ctcgtctacg ggtaccgtac tccgccgttc ggtgtgcggg cgcgaatgtc
     2701 gcacgacaac ggcagaacgt ggtcggaaac agaactcgtg gttcacggtt ccgctctttc
     2761 agcagactgc gggtacccga gtgctcttgt ggcggacggg tacgtgatca tcgtattcta
     2821 cgacgccggc aggaccacac acgatgctcc cgatgggact ggagcgttct gtgaactcgt
     2881 acggatcgcc gaatcagagc tgatttcggt gttttgagac ggaacggaat gattctgaga
     2941 agaagcgtac tcgtcttcgc actcctgtgg gcggggtacg tccctgcaga aaccatcacc
     3001 actcagtccg ggctgttccg cgtgcattac gcaccgggag ccaaagaaac cgcgaaacag
     3061 acggcaatta tcgcggaaga tgtcttctac cagctcgtca cggcatattc cctccacgag
     3121 cgattccggc ccattgatat cctcgtcacg gatgacattg acgcaggtaa cggcttcgct
     3181 gattactacc agaaccaact cgtggtctgg gcgacgaacc tggataccga tctccgcggc
     3241 acaaaccagt ggcttcggaa tgtcgtaacg cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nngacgaaat gggagtgttc gcccacaact ggctcaaatc ggagatggtg
     3421 tacaatcagg ggtacgcact gacgtcgtac atcagccgca attttggagc aaacgcacct
     3481 cgcaacctcg caaaaaacac cggatatgtg acgttcaata ccgctctgcg tgaaaatctt
     3541 cgacttaccg ggcaagacct ctacgaccgg tgggttgcgg atatccgggc gcggtattca
     3601 tcggtgcggg atagtgtcgg tgccattgtc gaggggaatg cgctgccgga cgaaggatca
     3661 tacgagacag cgcccgcgat ttcccccgac ggcacccaga tcgcctacct ttcggcgcga
     3721 ggggaagatt tccttctgac aaaaccccgc attcgtcatc tcgcgcgcaa cactgttctc
     3781 gacatcgatc gctctgtgcg cgggaacctc gcctggtttc ccgacggcgg caggatcgcg
     3841 tacgcgaaat tcgggagggg aacgtacctc aacgacctct acgtctacga catagcccgg
     3901 aaagaggaaa cgcgcatcac cagtcggttg cgcgcacgtg atcccgccgt ttcgccggac
     3961 gggaaatgga ttgcatttgt gtccggggaa gacggcggca cacggctcgg aatagtgcgt
     4021 gccgacggat cggaaatcag atggttgacg aacagcatgc gaagcccggg tccgggaccc
     4081 gccgacatcg gttccaaaac aagtgatctg attcagttct ataccccgaa atggtctcct
     4141 gacggcaaaa gtcttctctt cagcgtgttc cgggacggag atcgcgacat agggataata
     4201 ggaacacagg gtccttattt ctcggtgagg gacgctctgt cggattcggc cgcatttccc
     4261 gataccctcg tgtatccagc ggggtcaggt ttccggctgc tcgtccacac ggaagcagac
     4321 gaacgagacc ccgcgtggct gcccgatggg agcggatttg tcttttctgc tgactacgat
     4381 atcgcaacag atccacgcaa tcagacgccg gaagaaagcg tgcgttctga cggtgggtcc
     4441 agacctttcg gaatcttcaa cctctacctc gcaacgttgc cggcggattc cacagaggaa
     4501 atatcgtaca gacggttgac gaatgttctc ggaggtgcgt ttcggccggc ggttgctccg
     4561 gacggcagat ggctggcgta cgtgggttac cacgcgaaca acttcagcct ttacatcctg
     4621 ccattggcgg cagatggaca agccgcgtta aggccgccat ttgcaggtga cgcggtcccg
     4681 cgttcctacc agcatatcgc gaaatctcct cccgcttcgc agcttttcca cgttggtcag
     4741 acaacgggaa tgcggacgct ggtgggctgg gtcccggtgc ttcgcttcgg gcccagcgtc
     4801 atcggcgatc ggttttccgt gaaccatgta ggacttgggc tcctcgctgg aatcgaagat
     4861 cagatgggtg cgcggtatta tttcggcagc ggggagattt cgaaaaacat cgggaaacgc
     4921 gagccggcaa gcaccggggt gtttctgtac ggcgaccagg gattgcgtcc tctgctgaca
     4981 acagaatctg gcctttcgcc gcgtctctac gggtacggcg cacgtcagga aatctgcgcg
     5041 gtgacagagc gggccaggac cacccgcgcg tcggctcccg gtgctgttct tggagccaac
     5101 aacgaaacca tcccattgaa cccgctttct cgagtggaat tgagcgactc cgtggtcggg
     5161 tgggataatt accggtatct ctacggagga ctggggattc gcgcgggtgt cgggagccgc
     5221 cagaccgcgg cgtttgaact ggggtggcag aattactacg tggacgagcg actggatcgc
     5281 aggatcgaca attacagcgt ctttctcgac aatagcaatc cgccccagga cgtaaccgcg
     5341 ctgtttccgt ggggaaacta cgggtatcca aaccccggca ctcccctgat ctgggaccag
     5401 cgcccgcaat tttctgttgc ctatttcaga gacaggactg cttctttccg gtgggcttac
     5461 gcgaagatca ccccgaccgt agatgacttg gtgaatccca cgggcgggcg ggttatcact
     5521 gcatcatact cgttccaccg cgtaagcgtg atggattcgc tggcggggac caactacgac
     5581 gccaactaca atcaaatcga tccgacaagg ccgctgtatt cgcaggttga ccggcatttt
     5641 tccgcgaacg agctggtgtt gagctacgag gaatacatac ggttgcccgg agttctgaac
     5701 cgcacgaccc tcgcgttaca gggagtcgcc gcttatttcg accgtgcgct caagtactat
     5761 gaccctaacg acgaagggtc caatttcctg gagggatggg cgtactggcc attgcggtac
     5821 cgtctgggtg gcggcggcac gctccgcgga tatccctatt tctcgcacga aggaagcaaa
     5881 atggccttct tccgcgcgag ttatgtcttc ccgctattgc agcaccatcg caagcagttt
     5941 ctgtcatttt tccacgacag aacgtacggg gcgttgttcg tggaaacagg aagcacatgg
     6001 aactggacga ggctgaagga tgcgtggatc gcgcgggacg acttcctctg ggacctgggc
     6061 gcagaactga gaatttcggg ccggatgttc cactacatgc ctgtggtgag ctatgtggct
     6121 gtagcgcggc gcatgaagga cgttccttac ccgttcgtgg attatgagcg aacggcggaa
     6181 ggagcgattc ttttccggca acctgatcgt taccgcgtct atatcggtgc ggcgcttgcg
     6241 ctgggcggcg gcgggggagc ggccccccac gcggaaccac ggttcacacg cacgccgcag
     6301 gttctcatgc tctccaaccc ggcgcgccaa ccggccaatg gagcgtggga aagccccggc
     6361 acgactgcgg aaaaacttcc ccttcttttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn ngtatattcc gtgcggccga ggtcacgaag ataggagtgc tcgggaccga
     6541 cgctggcata gtcgaggccg gcggaaatgg agtgcgtggg agcaatctgc ccgtcttcat
     6601 tctgaaggac atagctcttc gacccttgca gcacaccaag tcgcccgccc ctgaatctcg
     6661 ccgcatggtc cccgagccgg gttcccctgc ctcccgcttc cacaccaatc agacgcacgt
     6721 tgtcgtcgcc caggaacgcg tgaaacaggc ccatcgcgtt cgaaccgccc cctacgcacg
     6781 ccaagcagac gtccgggagg cgaccgatct cctcctgcat ctgcgcccgc gcttcctttc
     6841 cgatgacgga ttggaaatca cgaactatca tcggatacgg gtgcggcccc agcacggagc
     6901 caaaaatgta gtacgtgtca tcgacacggg cgatccagtc tcgcatcgcc tcgttaatcg
     6961 catctttcag ggtttttgcg cctgaatgta ccgccgtcac tttcgccccg agcaaacgca
     7021 tacgaaagac gttgagcctt tgccggcgca tgtcttcggc acccatgtat acttcgcaat
     7081 caagaccgaa aagcgcgctg acggttgcgg ttgcaacacc gtgctgcccc gctcccgttt
     7141 ccgcgattat ccgccgcttg ttcatgcggc gcgccagaag cacctggccg atcgtgttgt
     7201 tgattttgtg cgcacccgta tgcgccaggt cctcgcgctt gaggaatatc cgcgctcctc
     7261 caatatggtc cgagagcctt ttggcaaacg aaattggagt ggggcgcccc acgtagtttc
     7321 ggagcagtcc cgaaaactcc tcctggaacc cggcatccgc tttcgctgct tcgtattccc
     7381 gcgcgaggtc ggaaagcaag ggcattaagg tttcgctcgc aaagcgccct ccgtagatgc
     7441 caaaatggcc cgtgttgtcc ggcacgctgt aggtcgaagc tggtacattg gtcacgtgag
     7501 agaagctcct gcggacgggt tcagccaagc gattccggcg accacgtgat gagtccggtg
     7561 gcgaacgggt tggagaggtc gggatggaac ggcaccacgc gaatcgcaaa tccatgttgc
     7621 cccgacccga tagccggaac gtccccttcg aaaaccgcgt cgctgccctg ggatacgaac
     7681 gacatgtttg tggcggtgcc ctcgacaatt tcgccgtggg agtcgagtcg accgtggtac
     7741 acctgaaccg aaacatccgc aggaagaaga tttccgaggc tgaccgtcgc cagaacgtgc
     7801 aacatcttgc ccacaggcag ctcgtttaca tgatcgcttt cgatccgcgt cacacgtacg
     7861 ttgtgccatt cgcggaatac ccgggttttc catgcagcca gatcacgtgc ctttttgcat
     7921 tcgtcggcag taatgtcacg ccaccggttt tcggcatccc ggtagaaccg gcccgtgtac
     7981 tcgcatacca tgcgattggt gttgaaaaca ggacagagnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnncg ggcaacgttg atgtcatagt cttcgatgaa ggcaacgtga
     8161 cgccgcaact cgtcattgag cctgatgaaa tggatgattt tccgtatcaa ctccttcccc
     8221 ggagaatcag cggggtgggc ctttcccgcg aaaatgatct gcacggggcg ttccttgttc
     8281 gtcaggagcg ctatcaggcg atctggatcc ctcaaaagga gatctgctcg cttgtaagtg
     8341 gcaaagcgac gtgcgaagcc tacggtcagt acatcagggt taagtacctc gcctgcagac
     8401 tgaatttcgc gccgggtcgc gccccgtcgc tccaactggc tccgaaggcg ccgtctcgca
     8461 aatgcgacga gacgctcacg cctccgttcg tgggtacgcc agagttcttc gtccgggatg
     8521 cgggaaatcg cctgccagat cgtctgatcc gccggtcgct ccatccaacg ggtaccgagg
     8581 taacggttga acaaccatcc catatcgtta gagatccacg accgcgcgtg cacgccattg
     8641 gtaatggagg cgatgggtac ctcctccacg ggcaggccag gccagaggtg tttccacatt
     8701 ccccgcgcga cgcgcccgtg caacctgctg acgccattcg tgtgggccgc gaggcggatc
     8761 gccagcgcgg tcatgcaaaa ctcactgtgc acattcccgg ggttctcctg ccccaatgcg
     8821 aggaactgct cgcgggaaag ccccagcgcc gcgtaataac catacaggta tcggtcaatc
     8881 agttcgggag cgaatcgatc attgccggcg ggaaccggcg tgtgagtggt gaatacggta
     8941 ctcgcttcag tgatttccag cgcttccgcg aacggctttt tctcctcctg aatcatctcc
     9001 cgcagacgct ccaccgcgag aaacgccgag tgcccctcgt tcatgtggaa cacggtgggg
     9061 cggatgccaa gttctgcaag cgcgcgcttc cctcccacgc caagcaaaat ttcctgtttg
     9121 atgcgcattt cctgatttcc gccgtagagc tgatccgtga tgtctctgtc ctcccggctg
     9181 ttttccctga tgttcgtatc cagaaggtag acggggacac gtccgacctg gactttccat
     9241 attgccgctc tcaccgccct gcccgggagt gaaacctcca cgaaagcttg agttccgtca
     9301 tttctgacgg ctttctgcag cggcatcgag taaaagtcgt tgatcgggta gccctcttg
//
LOCUS       DAOTNG010000102         9350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6753_length_9350_cov_4.587305, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000102 DAOTNG010000000
VERSION     DAOTNG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6753_length_9350_cov_4.587305"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="P5179_06470"
     CDS             complement(<1..1053)
                     /locus_tag="P5179_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94897.1"
                     /translation="MRRSLVLAVLILLEGICGDAGASVGDWRSYSRLVIHRIAAGPDA
                     VWAATEGGIIRSSLVDGSARTYSPSDGLPDRSILSVIVDSAGTAWFGTGEGGILRFTR
                     RKNLSLPVLEPPLLSGQVSRVVALFLWQNRLYVGHSTGVTVLDRGTNRVVENYHQLGA
                     RSDLRNQAVSALAVVGGRIYAGTARGLSWAELDGNPLLPDSWTSVEDSFAGVSSIASF
                     HGKVYVATLNGVKVETTSGWVLTGPAASVYDLAVHDGALWMASSRGLLRTTDGVNWEA
                     IADLSSPVKALAPLEGVALIAAGEAGTYRVQGATPVVTKMPGVDFPPTNAFTNIVLDT
                     SGSVWVAPGPARGAAQY"
     gene            complement(1060..2709)
                     /locus_tag="P5179_06475"
     CDS             complement(1060..2709)
                     /locus_tag="P5179_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94898.1"
                     /translation="METCAKTDILTHRRWAWALGAGLAGFIVYFGVWNLAFASDDVAL
                     IYRMRFFGWDGIRPFLLPETGAQGVGYQAYYRPFVVLFTAGEYLFWGTAPGGYHLSNL
                     VVYSLTCSLVFLLVSEVVGSRFVGLATALLFAVHPLHTMNVAWVSGRTDLFASFFILG
                     SLLAFVHWSESGKKRWIWIGLSAEFLGLCSKEIALLTPFLAVGAEWARVSHQDTHPSV
                     ATVLRKTAAWWVLPLLWGAMIAGSSPFLHSFSWAVSLREAFVNLAGSVVLLISPLDYE
                     VLLGWFAERPVLLVCAGALCGAGALFAVWRTQGNRSTMLWGLIWLCVSIAPTYRATMR
                     WYLFLPSVGICMVLASVCASFSERWRVSLVALLVLAFALGLANERRKWAIADAANRSA
                     LSSLLANARGDSIGRVLILTTPAKVRRMPVFGGNTESFFRIHGGTAGGVDVISHLALC
                     DPLGVVKCSRRDATTMTLELTEGEGEFLPLRARAHLTERVVGVGDTIDSDAGTVVVAK
                     LSRTGGVRAVDVSFPRVAFRDSRILLFTRGRFVPFTLPVEE"
     gene            complement(2679..4241)
                     /gene="lnt"
                     /locus_tag="P5179_06480"
     CDS             complement(2679..4241)
                     /gene="lnt"
                     /locus_tag="P5179_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRS94899.1"
                     /translation="MNVRSRQVLLSLLSGVLLAAAFPPLPSGPLAWVALVPLLFALDG
                     ADTRRAAGLGYVFGVAFHVCSLYWIAFHVDIPRLLSIAAWVAASLVLGVYYAAACACF
                     RLTARWVGRRWVWFVPFIWVAIEYSRYVSEFAFPWTVIAHSQSRLLGLIQQADFWGVL
                     GVSLWVVALNVLAFRAMQEVPVNARRATGFAATFALVVAASATYGTWRMSENPTVPAR
                     TRIALVQPNIGMDTKWDADEGIRATMDSMMHQTMQIPPGSADLVIWPETAIPDYVVYA
                     PPFSLDSGRNINPRYVAYFDSLTRHLRAPILSGLPAQDFPRDAQFNSAALIIPGTSSV
                     QYYDKTFLVPFGERVPYESAFGFLGKMNLGIARWSPSNRFSVFHSPAGNFGVGICFES
                     VFPRVMSDFVRAGADFLVVVTNDAWFGKTSLIYQHAAFASFRAIENRVWVARCANTGI
                     SAFYDPWGRRVQEAGIFTRETLIGTIGPREKVTPYVLWGDWIVVIAGAVSLIGVAGGG
                     MAAWKRARRRTS"
     gene            complement(4249..5376)
                     /gene="mnmA"
                     /locus_tag="P5179_06485"
     CDS             complement(4249..5376)
                     /gene="mnmA"
                     /locus_tag="P5179_06485"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRS94900.1"
                     /translation="MAGERVIVAMSGGVDSSLAAVLLHEQGYRVAGLTLKLWDRNHAA
                     GLHGAGEAASHESEPAADARAVCERLGVRHHVLDFTEVFEQVVIDDFVREYGSGATPN
                     PCVRCNALVKWEALLRMAERLDAEFVATGHYARVIHREDGIAELRKGADTSKDQSYAL
                     WRLRQEHLRRTLLPLGGMRKTETRRMAAERGLCTANKPESQEICFIPDNDYVRFLREM
                     DERSGQKSPGFVPGELRTFRSNEPAGTHGGTAHFTIGQRKGLRVAFGRPRYVVRIDPP
                     THTVWIGDASDLETATVHAEQVNWSLGPAPDAGTRSTAKIRYLHVGASGRVFPAPGGS
                     MAFVFEEPQRAATPGQSLVVYDHDLVLGGGVIRPVPGEKAS"
     assembly_gap    5833..5932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5933..>7365
                     /locus_tag="P5179_06490"
     CDS             <5933..>7365
                     /locus_tag="P5179_06490"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS94901.1"
                     /translation="VGGRSSDSVLPFMNLQLGEGGIVIAIGWTGQWMARFRRGGAGIH
                     VAAGMERTHISLEPGESIRTPRILLIPWEGEDRYVGNNLLRKAILAHYTPRKQASGEP
                     VLPPVSHMTMSTYHRTGKVTLEGELGALERAKELGCEAYWIDACWFGTGNWGGETGQW
                     TIRKDVFPDGLKPVGEAAARSDMGFVLWFEPERVCVGTEIERDHPEFVLRGGPNPNFC
                     LLNLGYAPAREFICDRISSIIADSGVTIYRQDFNFQPLPYWRAADPPDRIGISEIRHI
                     EGLYWLWDELRRRHPGLAIDNCASGGRRIDLETTMRSFPLWRSDFSDVGGPGYGRMLQ
                     IACQIQTAGLSQWVPIHSAAVWTYSPYDFRSAMSTGVTLYNNITVLDFPADEAKKAIE
                     ELKRIRRFFLGDYYPLVPVSFATHDWCAYQFHVEDESAGIAMFLRRHESPFPLMETRL
                     RAIDSSARYEVTMSPGFERGVAREMRG"
     assembly_gap    7366..7465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7466..8089
                     /locus_tag="P5179_06495"
     CDS             <7466..8089
                     /locus_tag="P5179_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRS94902.1"
                     /translation="GEWEKVHGGAFTAESVTKAELLDLSDLGTGWRILDVPFSPRAGC
                     AAAAVGPRVYVFGGYTCQVEAGKRAFTYFDDTFSFDVNTGTWQQHSSLPGALSGHCAV
                     SWGDRYVVLIGGVFRQETCGQMLAMSGVRLLDKRKALTGEYSDLVWAYDTVTDTFTLM
                     EERSPHGLNDIHACVVGNTIFTVGGENVDPATSNTTDYVMLGKITGN"
     gene            complement(8096..8443)
                     /locus_tag="P5179_06500"
     CDS             complement(8096..8443)
                     /locus_tag="P5179_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS94903.1"
                     /translation="MSNDAPLGDRARALARNLHVLTVMFPDSEKPLLGMQLRNAAIEL
                     VTEAVVASATDDPAEKQEMLRLAAVNCTRIRELIQLARELDHLPEDDVAELIAAVEVI
                     NATIARERRTARY"
     gene            complement(8440..>9350)
                     /locus_tag="P5179_06505"
     CDS             complement(8440..>9350)
                     /locus_tag="P5179_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRS94904.1"
                     /translation="IRRQVVPSVAEIEDPDGVEDPLGEIEQSPVQNVIQIYPDRVALT
                     VVTQCPVFCRFCFRKRLFSTTRGGAVRGLEKSIEYIRGTPQIKDVLVTGGDPLIASDS
                     FLDRLLRSLRSIPHVEIIRIGTRAPVVLPQRITPRLCAILSRYHPLWVNTHFNHPKEL
                     TPEAREACDRLLRAGIPLGNQSVLLKGVNDDPETMRELVRGLVRMRVRPYYLFQCHLV
                     RGTRHFRTTIEQGLRITGALRGHVSGIANPLFVVDTPDGKVPLLPRRGLKRREGDYVV
                     LETFKRTEWREYNPTADGQRKGREDH"
BASE COUNT         1995 a         2860 c         2588 g         1707 t
ORIGIN      
        1 gtactgcgcc gccccacgtg ccggtccggg tgccacccag acgctgcctg aggtatcaag
       61 cacgatattt gtgaaagcgt tcgtaggggg gaagtccacc cccggcatct tcgttactac
      121 gggcgtcgcg ccttggaccc ggtaggttcc tgcctctccg gcggcaatca gggctacccc
      181 ctccaatgga gcaagcgctt tgacgggaga tgacaggtcc gctatggctt cccagttcac
      241 gccatcggtt gtccgcaaca acccgcgcga agacgccatc caaagggctc cgtcatgcac
      301 cgcgaggtca tagacagacg ctgcagggcc cgtcaatacc catccggacg tggtttccac
      361 tttgacgccg ttgagggttg ccacgtagac ctttccgtgg aacgaggcga tagagcttac
      421 ccccgcaaaa gagtcctcga cgctcgtcca ggagtcagga agaagtggat tgccatcaag
      481 ttccgcccac gaaaggcctc gagcggtgcc ggcgtagatt cgccctccca ccacagcgag
      541 agctgacact gcctggttgc gcagatcgct gcgggcgcca agctgatggt agttttccac
      601 cacccggttg gtcccacggt ccagaaccgt cactccggtg gagtgcccga cataaagccg
      661 gttttgccaa aggaacaatg ccacgacacg ggatacctgg ccactgagca gcggaggttc
      721 cagaaccggg agcgaaaggt ttttccttct tgtaaaccgg agaatgcctc cttccccggt
      781 gccgaaccac gcagtgcccg cactgtcaac gatgaccgac agtatactcc gatccggcag
      841 accgtcactc gggctgtacg tccgtgcgga gccgtccacg agactcgaac ggatgattcc
      901 tccttccgtt gccgcccaga cagcatccgg tcccgccgcg atgcggtgga tgacaaggcg
      961 gctgtacgaa cgccagtcgc caaccgatgc ccctgcatcg ccgcagatac cctccaggag
     1021 gatcagaacg gctagtacga gagagcggcg cacgaaacct cattcctcca ctgggagtgt
     1081 gaagggaacg aacctgccac gggtgaaaag cagaatccgg gagtctcgaa aagcgacacg
     1141 cggaaaactg acgtccacgg cacggactcc gccggttctg gagagtttcg ccacaaccac
     1201 cgtacctgca tcagagtcga tggtatcccc tactccgacc acacgttccg tcaggtgcgc
     1261 tctggccctc aagggcagga attctccctc accctcggta agttccagtg tcatcgtggt
     1321 cgcgtcgcgc cgggaacact tcacaacccc gagtggatcg cagagagcaa ggtgcgaaat
     1381 gacgtctacc cctccggccg tgccgccatg gatacggaaa aacgactctg tattcccacc
     1441 aaaaacgggc atccgccgca ccttggcagg cgttgtcaga atcagaaccc ttccgatgga
     1501 atcgccgcgc gcgttcgcca gaagagagga caatgcggag cgattggccg catccgcgat
     1561 ggcccacttc cgacgctcgt ttgcgaggcc gagcgcgaac gcgagcacca gcagggcaac
     1621 cagagagaca cgccacctct cggagaagga agcgcagaca cttgcgagaa ccatgcagat
     1681 accgacagag ggcagaaaca ggtaccagcg catggtcgcg cgataggtag gagcaatgct
     1741 gacacacagc cagatcagtc cccacagcat cgtggaacga ttaccttggg tgcgccacac
     1801 ggcgaaaaga gcacccgcgc cgcacagggc acccgcgcag acgagcagca ctggtcgttc
     1861 ggcaaaccag cccaacaaca cttcataatc aaggggagaa ataagaagga caaccgaacc
     1921 ggcaaggttc acgaacgctt ctcgaagaga cactgcccac gaaaaagaat gcaggaacgg
     1981 gctgctgccg gcaatcatcg ctccccacag aagcggcaac acccaccacg ccgccgtttt
     2041 ccgcaacacg gtggcgacgg aggggtgtgt gtcttggtga gaaacccttg cccattcagc
     2101 tccgacggcg aggaagggag tcaaaagcgc gatttctttg ctgcacagcc ccaggaattc
     2161 ggcggacagg ccgatccata tccagcgctt cttccctgat tcgctccagt gcacgaatgc
     2221 aagaagcgat cccagaatga aaaacgatgc gaacaggtca gtcctgccgc ttacccatgc
     2281 cacattcatc gtgtgcaacg ggtggacagc gaacaggaga gcagtcgcga gtcccacgaa
     2341 ccgtgagccg acgacctctg acacaagcag gaagaccagc gaacacgtga ggctgtagac
     2401 gacgagattg ctgagatggt acccccccgg cgctgttccc cagaacaagt attcaccagc
     2461 agtgaaaagg accacaaacg ggcggtagta cgcctggtat cccacaccct gcgcaccagt
     2521 ctccggaagc agaaacggcc tgatgccgtc ccacccgaag aaacgcatgc ggtaaatcag
     2581 ggcgacatca tcggacgcga acgccaggtt ccataccccg aagtacacaa tgaatccggc
     2641 cagacctgcg ccgagagccc acgcccaacg gcgatgggtc aagatgtccg tcttcgcgca
     2701 cgtttccacg cggccatccc tccgcccgct acgccgataa gcgagactgc gccggcaatg
     2761 acgacaatcc agtcacccca cagtacgtaa ggcgtgactt tctcccgcgg acctatagtg
     2821 ccaatcagtg tttctcgagt gaaaatcccc gcttcctgaa ctcttctccc ccacggatca
     2881 tagaaggctg agattcccgt attggcgcag cgggccaccc acacgcgatt ctcaatcgcg
     2941 cgaaatgagg caaacgccgc atgctggtag atcagcgatg tcttgccgaa ccacgcgtcg
     3001 ttggtcacaa cgacaaggaa atccgctccc gcgcgaacaa agtcgctcat caccctcgga
     3061 aacaccgatt caaaacagat ccccaccccg aaattccccg ccggggaatg gaataccgag
     3121 aagcgattgc tcggcgacca gcgtgcgata cccagattca tctttccgag gaacccgaac
     3181 gcgctttcat acggaacccg ttccccaaaa ggcacgagga acgttttgtc gtagtattgc
     3241 acgctgctgg tcccgggaat tatgagcgct gcggagttga actgggcatc acgcggaaag
     3301 tcctgagccg gaagtccact caggatgggc gcgcgaagat gccgcgtcag gctgtcaaaa
     3361 tacgcaacat aacgcgggtt gatgttcctt ccggagtcaa gactgaaggg aggcgcatat
     3421 acgacgtaat ccgggattgc cgtctcgggc cagatcacga ggtcggcgct tccaggagga
     3481 atctgcattg tctggtgcat catggagtcc attgtggcgc gaatcccctc atccgcgtcc
     3541 catttggtat ccattccgat gttcggttgc accagcgcaa tgcgagttct tgcgggtact
     3601 gtgggatttt cggacatgcg ccacgtaccg tacgttgccg atgccgccac aacgagcgca
     3661 aaggtcgcgg cgaaacccgt ggctcgccgc gcgttgaccg gcacttcctg catcgctcga
     3721 aacgcaagca cgttcaacgc tacgacccag agcgaaaccc cgagaacacc ccagaaatct
     3781 gcctgttgaa tcaggccgag caaccgtgat tgcgagtggg cgatcaccgt ccacggaaac
     3841 gcgaactcgc tcacgtatct cgagtattcg attgctaccc agatgaacgg cacaaaccag
     3901 acccatcttc ggccgaccca gcgagcggtc agtcggaaac aggcgcaagc ggcggcatag
     3961 tagaccccca gaacaaggct ggcagcgacc catgcagcaa tgctgagaag ccgtggtatg
     4021 tcaacgtgaa atgcgatcca gtagagcgaa cacacgtgaa acgccacgcc gaagacgtac
     4081 cccaaccccg ccgcccgcct ggtgtccgcg ccatccaacg cgaagagcaa tggaaccaga
     4141 gctacccagg ccagggggcc tgaagggagt ggcggaaacg cggccgcaag aagtactccg
     4201 ctcaggagac tgagtaacac ctgcctcgaa cgtacgttca tgattccttc aggaagcctt
     4261 ctcgccggga acaggtctga tcacgccgcc gccgagaaca aggtcatgat catatacgac
     4321 aagcgactgc ccgggcgtcg ctgctcgttg cggttcttcg aagacgaacg ccattgaccc
     4381 gcccggagcg ggaaagactc ggccgcttgc cccgacgtgc aggtatcgga tcttcgccgt
     4441 gcttctcgtt cccgcgtcgg gtgcgggtcc gagagaccag ttaacctgct ccgcatgtac
     4501 agtagcggtt tcaagatcgc tcgcatctcc gatccagacg gtgtgagtcg gtgggtctat
     4561 ccggacaacg taacggggcc ggccaaaggc tactcgcagt ccttttcgct ggcctatggt
     4621 gaaatgtgct gttccgccgt gggtccctgc cggttcgttg cttctgaacg tgcgcaactc
     4681 acctggcacg aagccgggtg acttctgtcc ggaacgctcg tccatttccc tgaggaagcg
     4741 cacgtagtca ttgtcaggga tgaaacagat ttcctgactt tcgggtttgt tcgcggtgca
     4801 aagcccccgt tcagcagcca ttctccgcgt ttcggttttc ctcatccccc ccagcggcag
     4861 cagtgttcgc cgcaggtgtt cctgcctgag tctccacagc gcgtacgact gatccttgct
     4921 ggtgtctgct cccttcctga gctccgcaat cccgtcctca cgatgaatta cccttgcgta
     4981 atggcccgtt gcgacgaatt cggcgtctag acgttccgcc atgcgcagca acgcctccca
     5041 tttcaccagt gcattgcacc gcacgcacgg attcggtgtc gcgccgctcc cgtattcccg
     5101 gacaaagtca tcaattacca cctgttcgaa aacttccgtg aaatcaagca cgtgatggcg
     5161 cacgccaagg cgttcgcaca ccgcccgggc atcggccgca ggctcagatt cgtggctggc
     5221 agcttcaccc gcgccatgca ggccagccgc gtggtttcga tcccacagct tcagggtgag
     5281 gcctgccacg cgataacctt gttcgtggag cagaacggcc gcaagggagc tgtccacgcc
     5341 cccgctcatc gctactatta ccctctcgcc agccatccct caacctgcac accggaaatg
     5401 agcgaccacg gcctgccctg cagacgtgcg catctcgctc aaaatgccac actttggaaa
     5461 gataccgacg tgagctcaga cggccaactt ccggattgcg gccactgaaa cggctccctt
     5521 gacacccctc gcccctgcag agtcgggcgt gttatactgg tatctcatcg gggaaagacc
     5581 gcgcccttca gcctgccggg cttgcccgcg aagcgttccc atgccatcaa tggcgccaca
     5641 gtcaggggga tcatagtgac cacattgccg gatgacatca gcccgtactt ctcctttacg
     5701 ttcggaggta cccgatcccg ggatctcctg ccttcctggc gcctcgaaac tgacacggtg
     5761 cgacgcgacg acgggttgga gattgtgtcc acgtgggctg gcgagccatc gggaatccgc
     5821 gcgatccagc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgggcgg
     5941 gaggtcatcc gattcggtcc tgcccttcat gaatctccaa ctcggagaag gtgggattgt
     6001 aatcgccatc ggctggacgg gccagtggat ggcgcggttt cggcgcggag gcgcgggcat
     6061 tcacgtcgca gccggtatgg aacggacgca catctcgttg gaaccgggtg aaagcatcag
     6121 aacacccaga atcctgttga ttccctggga gggcgaggat cgttacgtcg ggaacaacct
     6181 cctcagaaag gcgatcctgg cacattatac cccccgcaaa caggccagcg gcgaacctgt
     6241 ccttccgcct gtttcccaca tgacaatgtc cacctaccac cgcaccggca aggtaactct
     6301 cgaaggcgag cttggcgccc ttgaacgagc aaaagagctc gggtgcgagg catactggat
     6361 cgacgcgtgc tggttcggaa ccggcaactg gggaggcgaa acaggccagt ggacaatcag
     6421 gaaggacgtc ttccccgatg gcctcaaacc ggtgggtgaa gcggcggcca gatccgacat
     6481 ggggttcgtc ctctggttcg aaccggaacg tgtatgcgtg ggaaccgaaa tcgaacgcga
     6541 tcatccggag ttcgttctcc gtggcggccc gaacccgaac ttctgcctcc tcaacctcgg
     6601 ctacgcgccc gcgagggaat tcatctgcga ccggatctca tcaatcatcg ctgattcagg
     6661 tgtaacgatt taccggcagg acttcaactt ccagcccctg ccgtactggc gcgcggccga
     6721 cccacccgac cgaatcggca tttcggaaat ccgccacatc gaaggcctgt actggttgtg
     6781 ggacgagctt cgccgacgcc accccggcct ggcaatagac aactgcgcca gtggcggcag
     6841 gcgcatagat ctcgaaacaa caatgcgctc gtttcccctc tggagaagcg atttctctga
     6901 cgtgggcggc ccagggtacg gcagaatgct gcaaatcgcg tgtcagatcc aaactgccgg
     6961 gctctcgcag tgggttccga ttcattccgc agccgtgtgg acatattccc cgtacgactt
     7021 ccgaagcgcc atgtccacag gagtgacgct gtacaacaac atcaccgtcc tcgatttccc
     7081 ggcagacgag gcgaagaaag ccatcgagga actgaaacga atcaggcggt tttttcttgg
     7141 cgattactac ccgctcgtac cggtttcgtt cgccactcac gactggtgtg cgtaccagtt
     7201 ccatgtggag gacgaatccg cgggaatcgc aatgttcctc cggcgccatg aaagcccgtt
     7261 ccccctcatg gaaacccgcc ttcgcgcaat agactcctcc gcaaggtacg aggtcaccat
     7321 gtcaccaggg ttcgaacgcg gagttgccag agagatgcgg ggcgannnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnggcga atgggagaag gtccatggag gggcattcac
     7501 tgctgaaagc gtcacaaagg ccgaactatt ggacctgagt gaccttggaa cggggtggcg
     7561 aattctggat gtgccgtttt ctccacgtgc gggatgcgcg gcggccgcag taggtcccag
     7621 ggtgtacgta ttcggtgggt acacgtgtca ggtggaagcg ggaaagcgtg cgttcacgta
     7681 tttcgatgac acgttttcct ttgacgtgaa caccggcacc tggcagcagc attcttcgct
     7741 tcccggtgcc ctttccggcc attgtgcggt ctcatgggga gaccggtacg tggttctgat
     7801 cgggggagtg ttccggcagg aaacctgcgg ccagatgctg gcgatgagtg gggtaaggct
     7861 gctcgacaaa cggaaggcgc tgacagggga atacagcgat cttgtctggg cgtacgacac
     7921 cgtcacggat acgttcacgc tcatggagga gcgctctccc cacggactca acgatattca
     7981 cgcgtgcgtc gtgggaaata cgattttcac ggttggcgga gaaaacgttg atcctgccac
     8041 aagcaacaca acggattacg tgatgcttgg gaaaatcacg ggcaattgat ctcgttcaat
     8101 aacgcgctgt cctccgttcg cgggcgatcg tcgcgttgat gacctccacc gcggcaatca
     8161 gctctgcaac atcatcctca ggcaggtgat ccagttcccg cgccagttga atgagttccc
     8221 gtatccgggt gcagttgacg gccgcaaggc gcagcatttc ctgcttctca gccggatcat
     8281 ccgttgcgct cgccaccact gcctcggtca ccagttcgat cgccgcattg cgcagctgca
     8341 ttcccagcaa cggtttctct gaatccggga acatgacggt cagaacatgg aggttcctcg
     8401 ccaacgctcg tgcccggtca cccagtggtg cgtcgttgct caatggtctt cccttccttt
     8461 cctctgcccg tctgccgttg ggttgtattc gcgccattca gttcgcttga acgtctccag
     8521 aacgacgtaa tcgccctcgc gtcgtttcaa cccgcgtctg ggcaagaggg gaactttgcc
     8581 gtccggtgtg tcgacgacaa acaacggatt cgcgataccg gagacatgcc cccgcaatgc
     8641 accggttatc cgaagaccct gttcgatggt cgttcgaaaa tgtcgggtcc ctcgcacaag
     8701 atggcattgg aacaggtaat acgggcggac gcgcatccgt acaaggccgc gcaccagctc
     8761 gcgcatggtt tccgggtcgt cgttcacccc tttcagaaga accgattgat ttcccagcgg
     8821 gatcccggcg cgcaaaagcc tgtcgcaggc ttcccgcgct tccggcgtga gctccttcgg
     8881 atggttgaaa tgcgtgttta cccatagcgg atggtaccgc gacagaatcg cacacagccg
     8941 cggggtaatt ctctgaggaa gaacaaccgg cgcacgtgtc ccgattcgaa tgatttccac
     9001 gtgcggaatg cttcgcaacg atctcaagag ccggtcgaga aagctgtctg acgcaatcag
     9061 aggatctccg ccggtgacaa gcacgtcttt gatctgcggc gtcccgcgga tgtattcgat
     9121 gctcttctcc aacccccgaa ctgctccccc ccttgtcgtc gagaacagac gtttcctgaa
     9181 acagaaacga cagaaaaccg ggcattgcgt gacgacggtg agagccaccc ggtccgggta
     9241 gatctggatg acattctgga ccggagactg ttctatctct cccaaagggt cctccacgcc
     9301 gtccggatct tcgatttccg ccaccgaagg cactacctgt cgccgtatgg
//
LOCUS       DAOTNG010000103         9331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6768_length_9331_cov_5.518111, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000103 DAOTNG010000000
VERSION     DAOTNG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6768_length_9331_cov_5.518111"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..242)
                     /locus_tag="P5179_06510"
     tRNA            complement(169..242)
                     /locus_tag="P5179_06510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Arg,seq:tcg)
     gene            706..4266
                     /locus_tag="P5179_06515"
     CDS             706..4266
                     /locus_tag="P5179_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS94905.1"
                     /translation="MPRSMSSYQRWFVAAGIAFLSAVSLLRAGTTGKITGIVRDQSGQ
                     PLPGVAVQISGLRLGGVTDADGRYFILQVPPGTHEVQANMVGYRTSITRDVLVGADRT
                     TTLNFTLREETIEVEAVVVTAGRPPIEVDVTSSQTTVNSARVAEVPVNQMLDVLAYEP
                     GVSIARDNELNIRGGGATQIRFQVDGLDRTDGLTNKPYTQLNQTLVAEVTLLTGGFNA
                     EYGNVRSGMVNVVTKAGNERGPNRGWVSGVYSMTPTARKKHFGPGGYSEDQFDYRTAL
                     LSDANTVGATGAADPYGAIYWPNLYESTRTDTAFRRVWTAAPNTYKVFDGWASRVTNA
                     NATNRGAGVYGKGTRTKPWTADQIIEAWRYEANLNENVWGYSHEPDWSLDLSAGWGLP
                     RKLGGIVIGYVTNKEMTITPALRPYYRDETFDVKLTLTPVDRLKWSFSYMHGTGVSTG
                     NGTSSYDPEMAETAGSIGIGGDAVSLRTAGDLLGTVYMSKGSYGNNRLNLSYNSFLDA
                     TFWQWGTALTYTISPATFATAAFGQTHTEYELVRDWPRADISDFSTTGTYKPSSTFSY
                     RAWLQMFALSWSDVNGDGRGDLPNDLNDALTPGRAFLEYPYGFPSAYKSVPSQTVYVN
                     RQFVFAPGDTARVVSPQGWVQEGYYDLSGTFWLGEGGQQYMTSHAVQTAARADITHAM
                     GSHTLKTGAEYLLADLEYHAEEASRIIGSRSNSNYRDYGGKFPASQPSILGIFLQDKF
                     ESQGMVMNFGLRAERFDGGAPVFMPDMIYDTRLFSPNNGKYWFDSLCVANGWNVNDPA
                     WGALPGSIGEAWSKFEKNGILDKFPMPYDVVAALPQRPAKAQWRLAPRFGISHPVSVR
                     TKFFFNYGIFYSMAKPAQMYGLGEHDGRTGALGRFEQLYYPELRPAKTTMYEVGFEQV
                     LPFSFVFKTTGYAKYNVDQVTGVQIHAGATDSYRTYRNANYQDIRGLEMKLARNGRFV
                     SGWATYQIIATRSGQTGLTDLYQNPTMNVYYTPLVTTNSPANNIAASVRVGTPVEWGL
                     LTGGWGASIIGRYNENTGEVIYNPNNQPRRELPPEWIMRGRDYWGADLKFTKDVAIGR
                     QRRASVYLDVTNFLNRKYLNGTYMNGNDYLAYVVERRAQGEKDLRVGDPSTWDALTQP
                     YRIRKADGTYTQWKAPISPRTDWIMFLYPRAYRAGIRFDL"
     gene            4307..6640
                     /locus_tag="P5179_06520"
     CDS             4307..6640
                     /locus_tag="P5179_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS94906.1"
                     /translation="MTATMRRWFTWAGMMGALVLTFSSTVMANTPQSVMHLGRIWHHP
                     EYDGAEGWSGSGPIYPAGIPTLPDGDNTVKRGWMGQVQKFGVYLTTTDWTSPLNTALE
                     YTSSYCFRSMDYAYYRVYVTEKGDYNFPVGCQEVFRWTRPTIVAGDSTLKFFPGPGPA
                     AYPDNSGVGPRPTPVVDPTLVTEEAIDVTWRFSQGVEYRRRQYGYGITTAHQDYVLFD
                     TRFVNNGKCYGDTVLADPAPFTMTGQTIHKMIFGQAYDYRNNNAPGAKQVGKDNEGMY
                     IQPWGASLNSVALFYDLDSDDAAAPGPDWGDPSENAAMEGHLTGNAYMAIGPVFASKG
                     PGSLYGTNDPDQPAFRMMHNERGLDFAGKAYSPANSQELREFLTDGALQMPIDQSFRT
                     YAPTAAIKDEDPGATAILGYGPLGGDKALSPANVKVHGWDLAFGDSVRLVQVVAGGGI
                     DAYEGQRIGQYWNAQKAASAAPDTWMSAADQALVQTGMDTVKKALALAYWNFYGSFPA
                     NVDAAKKAAWGIPNYVNSKPAAYNQPFNVPDPPRPPAFFSVKALAKGGIVVRWTRESE
                     SAPDFDTGVNDFAGYRVYRWEASRLTKPVIVKQGPASSFWAVAQDEAGPGPAGFAYVD
                     DQVTTGTDYWYAVTAYDDGTQNWAEPGKSLESAYFWCWTGYTSKGVTAPTLNGQGVDV
                     ARANRFALEQNAPNPFNPSTTIRFAVPANGNVRLTIYDVNGRIVRTLVDGSVLAGSHE
                     VVWDGMDMNGRAVASGVYIYRLTTANNEMTKRMTLVR"
     gene            6873..7298
                     /locus_tag="P5179_06525"
     CDS             6873..7298
                     /locus_tag="P5179_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94907.1"
                     /translation="MNTRLRHWWIAGVAFAFVLIALPRVSHSAVSTLDSVIVIPNPYN
                     LSGRTYGPQPPGGSIEANERIRFANLPAPCTIKVYTSAGNLVATINHTGANTAVSDLE
                     NYYWSGRNADNQYIVSDVYIYVVESPTLGRKIGKFIVIR"
     gene            7436..8524
                     /locus_tag="P5179_06530"
     CDS             7436..8524
                     /locus_tag="P5179_06530"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94908.1"
                     /translation="MNSLKASWVIGFAACVVAATASAQTTVPGYTGTNYDPPTPKWKG
                     QAGMTFLQIGGSARAEGMGGAFNGIKGDPSAIFYNPAGMASLGKAGVYANQTNWLAEM
                     KVTHFVGAGRVGPAIVGATFVNMDYGDIAGAQIVSSTVDPRGYQSTGNLSPETWSAGV
                     FGAIQMTDRFSTGVHVKYCVQDFSPSRIYSFSTRDYLATENRNRVGTFAVDLGTQYVT
                     GLRNIALNMSLQNFAQPQKYVEVKFDLPVTYRVGLSAELFELVTGNELSGQRVMLYAD
                     GMDQKDVSLDVAMGAEYLADLSQFVPGVKVGLRAGRRAAVNQDGWLSFGGSVQFGMSG
                     YNARFDYAYNDYGPGLTANRVGLSLTMD"
     gene            complement(8597..>9331)
                     /gene="bshB1"
                     /locus_tag="P5179_06535"
     CDS             complement(8597..>9331)
                     /gene="bshB1"
                     /locus_tag="P5179_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRS94909.1"
                     /translation="ALAIGPHRDDVELSCGATLVKLRKRGYRVGIADLTAGEMGTRGT
                     AKERAEEARCAARALGITYRVNLGLPDARIEPDAHTMDALIRLIRATRPTLVLAPHPE
                     EGRHPDHGAAGRLVPDAVFRAGLAKWETGQPHHRPLRVIHYMMHSLFLPTFVVDVTEE
                     WDAKMAAIRCYASQLNVPGANPGGEPTYISSPHFLGTIEARGRYYGAQIAATYGEAFY
                     QRELMRVEDPMSLALGSVERFAQKNA"
BASE COUNT         1829 a         2623 c         3078 g         1801 t
ORIGIN      
        1 gctctgaggc gaatcggcgt ctacgaaccg agtggaggaa acgtgtgcca gatgttccgg
       61 tccgtctcga tacaccgttc tctgcttcgc ttcgcccgcc actcgacgac cggcacaatg
      121 aaaaaacagc ggctcacatt cgcatggaag ccgctgtttt tactgaaata cgcccggtgc
      181 gattcgaacg cacgacctgc ggattcgaag tccgacgctc tatccagctg agctacgggc
      241 gcatgacgat accctatttg tataatgtaa ggggtgggcc agttttgggt caattcacct
      301 gtggatcgca cacccggttg ccgcccgggc acttgaccgt cgcaggtctt ctgtgctaat
      361 ggttgtacat gtggaggcac acgtccgggg agtgatggtt gtgcggcacc aatatcccga
      421 gtaccaaaaa acgaaaatcg caaccacagg cttgacagtc tgaaatcagt gtgcgatact
      481 agatgcagac acatgggatg ccagtgagtt tcgtgtagga gggaaatcac gaaagaacac
      541 ccgtgtgaaa cggaactttc cacgagtata agtcaccatt tctctggtat ttgtgtggca
      601 cacagggcgc gatcccgtgc aggggtggag ttttttttct gcagggaacg gggcccggtt
      661 ggatagtttc tggttctgat cacctgtttc cgaggagggg acgtgatgcc ccgtagcatg
      721 tcttcgtatc agcgatggtt cgtagcggca ggaatcgcat tcctcagcgc cgtgtctctc
      781 cttcgcgcag ggacgaccgg gaagattacg ggcattgtcc gcgatcagag cggtcagcct
      841 ctgccgggtg tggcggtgca gatcagcggc ctgcgactcg gcggcgtgac ggatgccgac
      901 gggcggtatt tcatcctcca ggttcccccc ggaacgcacg aagtgcaggc caacatggtc
      961 ggctaccgca cttcgatcac gcgcgacgtg ctggtgggtg ccgaccgcac cacgacgctc
     1021 aacttcaccc ttcgcgagga gacgattgag gtcgaggcgg tggtggttac ggcgggaaga
     1081 cccccgatcg aggttgacgt aacgtcttcc cagacaacgg taaacagcgc ccgcgtcgca
     1141 gaggttccgg tcaaccagat gctggacgtg ctcgcgtacg aaccgggtgt ttctatcgcg
     1201 cgggataatg agttgaatat ccgtggcggt ggcgcgacgc agattcggtt ccaggtggat
     1261 ggacttgacc gcaccgacgg attgacgaac aagccgtaca cccagttgaa ccagactctg
     1321 gtcgccgagg tgacgctgtt gacgggtgga ttcaacgcgg agtacgggaa cgttcggtcg
     1381 ggtatggtga acgtggtgac gaaggccgga aacgaacgcg ggcccaaccg cggctgggtt
     1441 tccggtgtgt acagcatgac gccgaccgcc cgcaagaagc attttggccc gggcgggtac
     1501 agcgaggatc agttcgacta ccggacggct ctcctttcgg acgcgaatac ggtcggcgcg
     1561 acgggtgcgg cggaccccta cggcgcaatc tactggccga acctgtatga atccacgcgg
     1621 acggataccg cgtttcgccg ggtctggacg gcggcgccca atacgtacaa ggtgtttgac
     1681 ggatgggcaa gccgcgtgac caacgcgaac gcgaccaacc gcggcgccgg cgtgtacggc
     1741 aaaggcaccc gcacgaagcc gtggacggct gaccagatca ttgaagcgtg gaggtacgaa
     1801 gcgaacctga atgagaacgt ctggggttac agccacgagc cggactggag ccttgatctg
     1861 tcggcagggt gggggcttcc ccgcaagctt ggcggtatcg tgataggata cgtcaccaac
     1921 aaggaaatga cgatcacccc ggcgcttcgg ccgtactatc gcgatgaaac cttcgacgtg
     1981 aagctcacgc ttacgccggt ggacaggctg aagtggagtt tctcgtacat gcatggaacg
     2041 ggcgtttcca cggggaacgg cacgagcagt tacgatccgg aaatggcgga aaccgcgggc
     2101 tcaatcggga ttggcggcga cgcagtgtcg ctccgcaccg ccggcgacct gcttggcacg
     2161 gtgtacatgt cgaaaggcag ctacggcaac aacaggctga atctttccta caacagcttt
     2221 ctggatgcga cattctggca gtggggtacc gcgctgacgt atacgatcag cccggccacc
     2281 tttgccaccg ccgcgtttgg tcagacccac acggagtatg agcttgtccg ggactggccg
     2341 cgcgcggata tcagcgattt cagcacgacc ggcacgtaca aaccctccag cacgttttcg
     2401 tatcgcgcgt ggttgcagat gttcgcgctg agctggtcgg atgtcaacgg agacggcagg
     2461 ggcgatcttc ccaacgatct aaatgacgcg ttgaccccgg gccgtgcgtt ccttgagtat
     2521 ccgtacgggt tcccttccgc gtacaagtcg gttccctctc agaccgtgta tgtgaaccgt
     2581 cagttcgtgt ttgcccccgg cgacacggcg cgggtggttt cgccgcaggg ctgggttcag
     2641 gaagggtatt atgatctgag cggaaccttc tggcttggcg agggcggcca gcagtatatg
     2701 acctcacacg cggtgcagac ggcggcccgc gctgacatca ctcatgcgat gggtagccac
     2761 acattgaaga cgggcgcgga gtatctgctt gccgaccttg agtaccacgc ggaggaggcg
     2821 tcccggatta tcggttcgcg gagcaactcc aactaccgcg attacggcgg gaaattcccc
     2881 gcgtcgcaac cgtcgattct cggcatcttc ctgcaggaca agttcgaatc gcaggggatg
     2941 gtgatgaact tcggtctgcg ggcggagcgg ttcgacggcg gggcgccggt gttcatgccg
     3001 gacatgattt acgacacgcg gttgttcagc ccgaacaacg ggaagtactg gtttgacagc
     3061 ctctgcgtgg cgaacgggtg gaacgtgaac gacccggcat ggggggcgtt gcccggttcc
     3121 atcggcgagg cctggagcaa gttcgagaag aacgggattc tggacaaatt cccgatgccg
     3181 tatgacgtgg tggcggccct gccgcagcgc ccggcgaagg cccagtggcg tctggcgccg
     3241 cggtttggga tctcccaccc ggtgagcgtg cggacgaagt tcttcttcaa ctacggcatc
     3301 ttctactcca tggccaagcc ggcgcagatg tacgggctgg gcgagcatga cgggcgcacg
     3361 ggtgccctcg ggcgttttga gcagttatac tacccggagc tccggcccgc aaagaccacc
     3421 atgtacgaag tagggttcga acaggtgctc ccgttcagtt tcgtgttcaa aacgacgggg
     3481 tacgcgaagt acaacgtgga ccaggtgacg ggggtgcaga ttcacgccgg tgcgacggac
     3541 tcgtaccgca cgtaccgcaa cgcgaactac caggatatcc gcggtctgga gatgaaactt
     3601 gcccgcaacg gccggttcgt aagcggatgg gcgacctatc agatcatcgc cacgcgaagc
     3661 gggcagacgg gattgacgga tctctatcag aatccgacga tgaacgtgta ctacacgccg
     3721 ctggtgacga cgaattcgcc ggcgaacaac atcgcggctt cggtgcgcgt aggcacaccg
     3781 gttgaatggg gcctgttgac gggcggctgg ggcgccagta ttatcggacg gtacaacgag
     3841 aacaccggcg aagtgattta caaccctaat aaccaaccgc ggcgtgaact tccgcccgag
     3901 tggatcatgc gcgggcggga ttactggggc gccgacctga agttcacgaa ggacgtggcg
     3961 atcggccggc agcggcgcgc atcggtgtat ctggatgtga cgaatttcct gaaccgtaaa
     4021 tatctgaacg gcacctacat gaatggcaac gattatcttg cctacgtggt cgaacggaga
     4081 gcacagggcg aaaaggacct gcgtgtggga gacccctcca cgtgggacgc gttgacgcag
     4141 ccgtaccgca tcagaaaggc ggacggcacg tacacgcagt ggaaagcgcc tatcagcccg
     4201 cgcacggact ggataatgtt cctgtatccc agagcgtacc gtgccggtat caggtttgat
     4261 ctgtagcacc gccaactggg ttgacgcatc agaaaggagg ccaataatga cggctactat
     4321 gcgtcgttgg ttcacgtggg ccggtatgat gggggcgttg gttctgacgt tctcgtcaac
     4381 ggtaatggca aacacgccgc aatcggtcat gcatctcggc cgaatctggc accatccgga
     4441 gtacgacgga gccgagggct ggtcgggctc gggcccgatt tatcccgcgg gtattccgac
     4501 gttgcctgac ggcgacaaca ccgtcaagcg cgggtggatg ggtcaggtgc agaagttcgg
     4561 cgtgtatctc accacaacgg actggacaag cccgttgaac accgcgctgg agtatacatc
     4621 gtcctactgc ttccgcagca tggactatgc gtactaccgc gtgtacgtca ccgaaaaggg
     4681 tgactacaac ttcccggtgg ggtgtcagga agtcttccgc tggacgcgtc cgacgattgt
     4741 cgcgggcgat tccacgctca agttcttccc cggacccgga ccggcggcgt acccggataa
     4801 ctccggcgtc ggccctcgtc cgactccggt ggtggatccg acccttgtga cggaagaggc
     4861 gattgacgtg acctggcggt tcagccaggg tgtcgagtat cgccgtcgtc agtacgggta
     4921 cggcatcacc acggctcatc aggactacgt gctgttcgac acccggttcg tgaacaacgg
     4981 caagtgctat ggcgacacgg ttcttgccga tccggctccc ttcacgatga cgggacagac
     5041 catccacaag atgatcttcg ggcaggcgta cgactaccgg aacaacaacg cgccgggcgc
     5101 gaaacaggtc ggtaaggaca acgaaggcat gtacatccag ccgtggggcg cctcgttgaa
     5161 ctcggtcgcc ctgttctacg atctggacag cgacgatgcc gccgctcccg gacccgactg
     5221 gggcgacccg agcgagaacg ccgcgatgga aggccatctg acgggcaacg cgtacatggc
     5281 gatcggtcct gtgttcgcaa gcaagggccc cggcagcctg tatggcacga acgaccccga
     5341 tcagccggca ttccggatga tgcacaacga gcgtggtctg gacttcgccg gcaaggcgta
     5401 ttccccggcc aactcgcagg aattgcgtga gttcctgacc gacggcgccc tccagatgcc
     5461 gattgatcag agcttccgca cgtatgctcc gactgccgcg atcaaggacg aggatccggg
     5521 tgctaccgcc attctgggct acggcccgct gggcggcgac aaggcgctga gccccgccaa
     5581 tgtgaaggtt catggttggg acctcgcgtt tggcgattcg gttcgtctgg ttcaggtggt
     5641 tgcgggtggt ggtattgatg cgtacgaagg tcagcgtatc ggccagtact ggaacgcgca
     5701 gaaggctgcc agcgccgctc cggatacctg gatgagtgcc gcggatcagg ctctcgttca
     5761 gaccggtatg gacacggtga agaaggccct cgcgcttgcg tactggaact tctacggctc
     5821 cttcccggcc aatgttgacg ccgcgaagaa agccgcttgg ggtattccga actacgtgaa
     5881 ttcgaagcct gccgcgtaca accagccgtt caacgttccc gatcctccgc gtccgccggc
     5941 cttcttcagt gtgaaggcgc tggcaaaggg tggtatcgtg gtgcgctgga ctcgtgaatc
     6001 ggaatcggct cccgacttcg ataccggcgt gaacgacttc gccggatatc gcgtgtatcg
     6061 ctgggaagcc tcccgtctga cgaagcccgt catcgtgaag cagggtccgg cgagctcctt
     6121 ctgggcagtt gctcaggatg aagcgggtcc cggcccggct ggcttcgcgt atgtggacga
     6181 ccaggttacc accggtacgg attactggta tgccgtgacg gcgtacgacg acggaacgca
     6241 gaactgggcg gaaccgggca agtcactgga aagcgcgtat ttctggtgct ggaccgggta
     6301 cacctcgaag ggcgtaacgg cgccgacgtt gaacggtcag ggtgttgatg ttgctcgcgc
     6361 gaatcggttt gcgctcgagc agaatgcgcc gaacccgttc aacccgtcca cgacgatccg
     6421 gttcgcggtt cccgcgaatg gcaatgttcg tctgacgatc tacgacgtaa acggccgcat
     6481 cgttcgcacg ctggttgatg gcagcgttct tgccggcagc cacgaagtgg tgtgggatgg
     6541 tatggacatg aacggtcgcg cggttgcgag cggcgtgtac atctatcgcc tcacgacggc
     6601 gaacaacgag atgacgaagc ggatgacgct ggtgcgttag tccggagttg gcggaacacc
     6661 ggtaccgggg acggcagaga gccgtccccg gtactgacag ataaacgatt gaagcaacag
     6721 gcgaacgagc acatcggaga gcgggcagcg cgcaggcttc tccggtagac gtgtcgcttt
     6781 ggccgggcga aggggtgccg tggaggcgca gtgatggcca gaggaccacg tatactatgc
     6841 ccgcgaagac gcaccggaaa ggacttggtg agatgaatac ccgattacgc cactggtgga
     6901 tagccggagt cgcgtttgca ttcgtgctga tcgcattgcc gcgagtttct cacagtgcgg
     6961 tgagcacgct cgacagcgtt atcgttatcc cgaatccgta taacctgtcg gggcgcacgt
     7021 acggtccgca gcctcccggc ggttcaatcg aggcaaacga gcgtattcgt ttcgccaacc
     7081 tgccggcgcc ctgcacgatc aaggtctaca cgtccgcggg gaaccttgtg gcgacgatca
     7141 atcacacagg ggcgaatacg gccgtttcgg atcttgagaa ctactactgg agcgggcgca
     7201 acgcggacaa ccagtatatt gtgagcgacg tgtatatcta tgtggtggaa tcacccacac
     7261 tcgggcggaa gattggtaag ttcatcgtga ttcgttagcc tggctgaccc gtggtggttg
     7321 ccccgtagtg gctgaccggt gggtcagcac gggtgacgct tcgtcagcag gctggcggca
     7381 cagatagatg atgtgcagcg gactggctct cggtgcgctg agaaggagct tctggatgaa
     7441 cagtttgaaa gcgagttggg tgatcgggtt tgccgcctgt gtggtggcag cgactgcgtc
     7501 cgcgcagacg accgttccgg ggtataccgg cacgaactat gacccgccga cgccgaagtg
     7561 gaagggtcag gccggcatga cgttccttca gattggcgga agcgcccgcg ctgaggggat
     7621 gggaggcgcc ttcaacggga tcaaagggga tccgtcggcg atcttctaca atccggcagg
     7681 gatggcgtcg ctcgggaagg ccggtgtgta cgcgaatcag acgaactggc tggccgagat
     7741 gaaggtgacg catttcgtcg gcgcggggag agtcgggccg gcgattgtgg gcgccacgtt
     7801 tgtcaacatg gattacggcg acatcgcggg tgcgcagatc gtctccagca cggttgaccc
     7861 gcgcggatac cagagcacgg gaaacctttc cccggaaacg tggtcggcgg gtgtgtttgg
     7921 agccatccag atgacggacc ggttcagcac cggtgtgcac gtgaagtact gcgtgcagga
     7981 cttcagcccc agcaggatct actcgttctc cacgagagat taccttgcca cggaaaaccg
     8041 gaaccgcgtc ggaacgttcg cggttgacct gggcacgcag tacgtgacgg ggctgcggaa
     8101 catcgcgctg aacatgtcgc tccagaactt cgcccagcca cagaagtacg tggaagtcaa
     8161 gttcgacctc ccggttacct atcgcgtcgg cctttccgcg gagctgtttg agcttgtgac
     8221 gggcaacgag ttatccggcc agcgcgtgat gctgtacgcg gatggtatgg atcagaagga
     8281 cgtgtcgctc gatgtggcga tgggagcgga gtacctcgcg gatctttcgc agttcgtgcc
     8341 gggtgtgaag gtgggcctgc gtgccggacg tcgcgcggcg gtgaaccagg acggctggct
     8401 gagcttcggc ggcagtgtgc agttcgggat gtcgggctac aacgcgcgct ttgattacgc
     8461 gtacaacgat tatggccccg gacttaccgc gaaccgtgtc ggcctgagcc tcacgatgga
     8521 ctgattcacg atctgagtgc aacgcgagcg ggcggcggga gcaattccgc cgcccgcttt
     8581 ttttgtgggg gggaggtcac gcgttctttt gcgcgaaacg ttccacggag ccgagggcga
     8641 ggctcatggg atcctccacg cgcatgagtt cccgctggta aaacgcttcc ccgtacgtgg
     8701 cggcgatctg cgcgccgtag taccgcccgc gcgcttctat tgtgccgagg aagtgcggag
     8761 aggagatgta tgtcggctcc ccgccggggt ttgcgccggg gacgttcagt tgcgatgcat
     8821 agcatcgaat cgcggccatc ttcgcgtccc attcctcggt gacgtccacc acaaatgtgg
     8881 gcagaaagag gctgtgcatc atgtagtgga tgacgcgtaa agggcggtga tgcggctgcc
     8941 cggtttccca cttggcgagt ccggcgcgga atacggcatc cggcacgagg cggccggcgg
     9001 ctccgtgatc cgggtggcgg ccttcctcag ggtgtggagc cagaacgagg gtgggacggg
     9061 ttgcgcggat gagcctgatg agtgcgtcca tggtgtgtgc gtccggttcg atacgggcat
     9121 cgggcagacc aaggttgacg cggtaggtga taccgagcgc ccgtgccgca caccgggctt
     9181 cttcagcgcg ttctttcgcg gttccacggg tgcccatctc tccggcggtc aggtcggcta
     9241 ttccgacccg gtatccgcgc ttcctcagtt tcaccaacgt ggcgccgcag ctcagttcca
     9301 cgtcatcgcg gtggggtccg attgcgaggg c
//
LOCUS       DAOTNG010000104         9299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6792_length_9299_cov_4.530506, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000104 DAOTNG010000000
VERSION     DAOTNG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6792_length_9299_cov_4.530506"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="P5179_06540"
     CDS             <1..914
                     /locus_tag="P5179_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94910.1"
                     /translation="LSFRPFEWLSLTYLHGSLQSGVPDTTSAWHRAAPSTAAFMSKYY
                     VVHRIAYTGIPGVEVGAGESLVYGGRGVAARYLIPVIPFRAAQHSEGDLDNLQMWGDL
                     SVSRLPWTKLYGSLFIDELSLNQLFKEDNIHNWWAWQYGAHVADFWGLIPDCDLFIEY
                     TRANPWVYRHRYPWNTYDTWSVRGNQPVTAYPLGFWQGHNGDYLRGEINWRPLRWLRV
                     GAWVSQARRGGDGSEIDQYNPPAEEFLFGPRTRTREIHVNCSVEWLRDFVLQCDMGRI
                     ARNADGNAGRTRKSWTSISAGIAYGVW"
     gene            954..2003
                     /locus_tag="P5179_06545"
     CDS             954..2003
                     /locus_tag="P5179_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS94911.1"
                     /translation="MNIREPVHFLRKSLRWGKLWNAAQALSSYGLSRIVRRPIIWGMP
                     PVLMVEPTNVCNLHCPLCPSGNGTLRRKRGHMDFDLFRRAIDEISGKSLMVLLWNQGE
                     SFLHRDFLRMVRYASEHGLYTLASTNGHYLNSPEEIVRSGLDSLIISLDGATPETYTR
                     YRVGGDFDRVIHGIRQLAATKRRLRSKLPIIHVQFILFRHNLHEIAKIRELAASLGAD
                     KVTYKTAQVYDGKEAEDFLPREPRFRRYQSVNGEVVSKAAARGSIPNRCRTLWVQPVL
                     NWDGTVTPCCFDKHGDFAVGYLSEETTLRRIWHGELMNAFRRRVMTDRGALAMCRNCT
                     EGIKTNYREEDIRIV"
     gene            complement(2068..3147)
                     /locus_tag="P5179_06550"
     CDS             complement(2068..3147)
                     /locus_tag="P5179_06550"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94912.1"
                     /translation="MNRGFCLRGAVCAALFAASSGVAQTTQYIKDGVYDPATPERRGQ
                     AGATFLQVGASARAEGMGGAFAGVRGDPAIAFYNPAGVAEVTGVAFSADHTKWVADMS
                     LNGLVVAARARFVSLGVSFLSMDYGSFDGTRIADNAAGYESIGAVTPTAWALGGIAAV
                     KFTDRFNAGIQTKWLVQDIGESEVYTFRNPPRYTGEMTKNRVSTWAIDLGTQYYTGLR
                     NLSINMTLQNFGGTQKYVETTFSLPLTYRVGVSAELMEFVTGDATGAHKVFLSMEGVD
                     RRNVSLDGAIGAEYTGDMSDFVPGLSVSVRAGRRPARNQDGWMSFGGGVCFPVTGVKM
                     GLDYSFNDYGPHLSVHRVGMRASLR"
     gene            complement(3188..3586)
                     /locus_tag="P5179_06555"
     CDS             complement(3188..3586)
                     /locus_tag="P5179_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94913.1"
                     /translation="MALFSRRWALPTAILVAFLCVFASDAISQTTTLDSVKVVPNPYN
                     VSGRTYASRSNIFGYERLRFANLPLPCTVKIYSSDANLVKTLQFNAGDLLPAWDGRNS
                     DNQYIVSDVYFYVVEHATLGTKIGKFVVIR"
     gene            complement(3648..6002)
                     /locus_tag="P5179_06560"
     CDS             complement(3648..6002)
                     /locus_tag="P5179_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS94914.1"
                     /translation="MNWRKGVLFAGVLGMVAVPAVFANGLQAQFHLGRFWTNPENEPA
                     ESSLMCYPGGVPRNEWSDGSGQQLKRSWLTHTRKHGGSMLTIDWTNELGTLYADAYSY
                     MYRGNDTSSPPRYRLDKPDAFNYLYAVSIQEYMRFPRPKLILVVKSPTSATGDTLIDF
                     IFFPGSAGLDQFKYPASGGKGPRPDVIVDPNLVTELKINTVWRYIMGVESIRDVYGYG
                     QTSPHQDYVFTDYKLVNNGISGRTANDHPPSDGATIQNQVINKMLWWHTRHLKDEMAS
                     VANQQCGDQEMMYVEPFGAGNHSIMMAWDVDLDDPTSPGPDWGDPIETAFFEGHLAGN
                     AYIVNGPVFASAGPGANYGVDDPAQPAFRTVWQEYGFDFGKTYSPADPAQAREMIADG
                     SLQMDLNVGFRDDPRFVNYVGGANIASAIWGYGKLAGPLNLANVRSQGYDMPWLDSLR
                     FVEVMGAGGIDVEEARRIGNAWNTNKLANPGDPASWMSAADIALIQTGRDTAMKACAL
                     SYWNFHGHMPANVTAADLAKWGLTGFVTSKPAQYGPFDAPDAPRPPGFLSVRPLNRVG
                     IEVRWGKEAETTPDHDTKVMDFAGYRIYRQTGTRMSPMVLIKQGPASDFAEVAADGAF
                     PAGRVFHDVNVTPGVDYWYAVVAYDDGTQNWELPGYSMESTRWWTWSGYMNKGATAPE
                     YVGVDASAAPGKFMLAQNTPNPFNPTTTIKFSLAGSAQTKLVVYSLTGQVVRTLINGQ
                     TAAGVHSVVWDGNDNMGRPVASGVYLYRLTSGTNEMVRRMVLVR"
     gene            complement(6148..>9299)
                     /locus_tag="P5179_06565"
     CDS             complement(6148..>9299)
                     /locus_tag="P5179_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94915.1"
                     /translation="VRAERPPIEVDVTSSQTVVNAARISEAPVSRMLDFLNYEPGVSV
                     TRGTELEIRGGGPSEIRFQVDGLDRTDGLTSKPYTQLNQVLVSEVTLLTGGFNAEYGN
                     VRSGMVNVVNKDGTERRSAAVALPWVSGVYNMAPARKKHFGPGGYDSDQYDYWLMSSD
                     SPFADSALAGPLYWPLLYEETRTAPEFTDPTNPKYVNPRTSAFRVFDGWKQRATVVNA
                     ARGWFGKRDWTPAEVREAWEWEANMNEQVWKYSNNPDYNLDLAAGWALPKRMGGVVVG
                     YSRTYEMTVVPALRPYNEDQTFEAKFTLTPLDKIKIQFSYMTGRNEGTAAGSTGGAAS
                     NPELAASGASVTGNDPVMLRASGDLLNGLTGTSDNTKFNLSYNSLLNGEYSQYGGTFT
                     YTFSPKTFLSASLGRSSSKWEVNRDLPRADFSDFAPGGRYQPPNTWSGQGWMGFAFGW
                     TDTNGVGGPDKPSSLADALNPNRLAPVSPFGIPYLYYQVPSEAKFIYREIGGDTTTVG
                     RVVSPQGYVMSAYKDLSGVYGIGGGGHVRLTGRSNQIVFRSDFTHAMGSHTLKTGVEF
                     IRGDLEYHMIQNSALTTNTEWRDYGGNYPAVNPSILGVYLQDKFETNGMIANGGIRIE
                     RFDAGQNAYFYNDMFDNDLFVNHGTRYFKDLSVALGWDTLAYGPVPVNNVSDYFKVKE
                     KLGVAPEPWDVVRIFPQSDNIVHWRIAPRFGISHPVSYRTKFFFNYGIFYSMQKAAVM
                     YGHSDHDGRAAFNGWVADMFNPNLRPAKTTMYEVGVEHVFPLGLVMTVRGYAKYNVDQ
                     VTRISVTNTLRSDFRGYSIFRNANYEDIQGAEIKISRSSGRFVNGWLTYQKTSSRSGM
                     VGLTVVDLNTVNIQPFESFASTSDPKGYFQGMLRVGTPMDWGNIRGGWAVSVVQSYAS
                     GGEVIYNPEALPRREIPDENILPSASSYNTDLKLSKAFQLGRGRSLSAYLDIGNVFNI
                     KRLNGGGIQNYIDYLGYIYGQRLQGNDMKVGDESTFAMFTRPWKDKDGNWKRPTSAQT
                     EWMHHLYPRSYRVGVRFDL"
BASE COUNT         1895 a         2879 c         2606 g         1919 t
ORIGIN      
        1 gcctctcgtt ccggcccttt gaatggcttt cgctgacgta ccttcacgga tcgttgcaga
       61 gcggcgtacc ggataccacc agcgcgtggc accgcgccgc cccttccact gctgcgttca
      121 tgagcaaata ctacgtcgtc cacagaatcg cgtacaccgg tatccccggc gtcgaggtcg
      181 gtgcgggcga atctctcgtg tacgggggcc gcggggttgc cgcccggtat cttatccctg
      241 tgataccttt tcgcgcagct cagcacagcg aaggcgacct cgacaatctg cagatgtggg
      301 gagatctgag cgtgtcgcgg ttgccctgga cgaagctgta cgggtcgctg ttcatagacg
      361 agttgagcct caaccagctc ttcaaagagg acaatattca caactggtgg gcctggcagt
      421 acggagcgca tgtggcagat ttctggggac tcatccccga ctgcgacctt ttcatcgagt
      481 acaccagagc aaacccctgg gtataccggc atcgctaccc ctggaacacg tatgacacgt
      541 ggtccgtgcg gggaaaccag ccggtgacgg cgtatccact tggtttctgg cagggccaca
      601 acggggatta cctccgggga gagatcaact ggcgcccgct gcgctggttg cgtgtcggcg
      661 cgtgggtgtc tcaggcgcga cgaggtggtg acgggagcga aattgatcag tacaacccgc
      721 cggcggaaga gttccttttt ggccctcgca cgcggacgag ggaaattcat gtcaattgca
      781 gcgtggaatg gcttcgcgat ttcgtgctgc aatgcgatat gggcaggatc gcacgcaacg
      841 cggatggcaa cgcggggcgc actcgcaaga gctggacgag catctccgcg ggcattgcat
      901 acggcgtatg gtgacgtgac aaaacccctc acacccggat tcccgcaaat cctatgaaca
      961 ttcgcgaacc cgttcatttc cttcgcaagt cgttgcgctg gggcaagctt tggaatgccg
     1021 cgcaggcact ttcaagctac gggttatcgc gaattgtcag gcgtccgatc atctggggca
     1081 tgcctcccgt tctgatggtc gagcccacga acgtgtgcaa tctccactgc cccctctgtc
     1141 ccagcggtaa cggaacgctg cggagaaaac gggggcacat ggactttgac cttttccgcc
     1201 gcgccataga cgaaatatcc ggcaaaagcc ttatggtgct cctgtggaac cagggagagt
     1261 cgttcctgca ccgcgacttt ctccgaatgg tacggtacgc ctcggagcac ggcctgtaca
     1321 cacttgccag cacaaacggc cattacctca acagccctga ggaaattgtt cggtccggcc
     1381 tggacagtct catcatcagc ctcgacggag cgacgcccga aacgtacacc agataccggg
     1441 tcggcggcga tttcgaccgg gtcatccacg gcattcggca acttgccgcc acaaaacggc
     1501 gcctacgatc gaaattgccc attatccacg tccagttcat ccttttccga cacaatctgc
     1561 acgaaatcgc caagatacgc gaactggctg cgtctctcgg cgcggacaaa gtgacgtaca
     1621 aaactgctca ggtctacgac gggaaagagg ccgaggactt cttgccgcgg gagccgcggt
     1681 tccgacgcta ccagtcggtc aatggagagg tcgtgagcaa agcagccgca agaggaagca
     1741 tcccgaaccg gtgcaggact ctgtgggtgc aaccggtcct gaactgggac ggaaccgtta
     1801 ccccctgttg tttcgacaaa cacggagact tcgcggttgg atacctttca gaggaaacaa
     1861 cactgcgccg catctggcac ggagaactca tgaacgcgtt cagaaggcgc gttatgaccg
     1921 acaggggagc attggctatg tgcaggaact gcacggaggg aataaaaaca aactatcgcg
     1981 aagaggacat tcgcatcgtc tgacggaagg ccggatcact cacctctccg gcctgcacac
     2041 tacccgcact tccttcgcca gatcggttca acggagcgaa gcccgcatcc caacgcggtg
     2101 gaccgaaaga tgcggcccgt aatcgttgaa cgaatagtcc agccccattt tgactccggt
     2161 cacgggaaag cacaccccgc cgccgaagct catccaaccg tcctgattcc gcgcaggcct
     2221 tcttcccgcc ctcactgata ccgaaaggcc cggaacaaag tcggacatgt cgccggtgta
     2281 ctccgcgccg atcgccccgt caaggctcac atttcgacga tcgacacctt ccatcgacag
     2341 gaacaccttg tgcgcgcccg tcgcgtcccc ggtgacgaac tccatgagtt ccgcagacac
     2401 gcccacgcgg tacgtgagcg gaaggctgaa ggttgtttct acgtacttct gcgtcccgcc
     2461 gaaattctgc agtgtcatgt tgatactgag gttgcggagg ccggtgtaat actgggtgcc
     2521 gaggtcgatc gcccaggtgc tcacccggtt cttggtcatt tcgccggtat accgcggcgg
     2581 gttgcggaat gtgtagacct cgctctcgcc gatgtcctgc accagccact tcgtctgaat
     2641 tccggcattg aaccggtcgg tgaatttcac cgccgcaata ccgcccagcg cccatgcggt
     2701 cggtgtcacc gcgccgatgg attcgtaccc ggcggcgttg tctgcgatgc gcgtgccgtc
     2761 aaaagaccca taatccatgc tcaggaacga gacgccaagt gagacaaacc gtgcacgcgc
     2821 cgccaccacc aggccattca gtgacatatc ggcaacccac ttcgtgtggt ccgccgaaaa
     2881 cgccaccccc gttacctccg ccacgccggc aggattgtag aatgcaatcg cgggatcccc
     2941 ccgaacaccc gcaaaggctc cccccatgcc ttccgcgcgt gcgcttgccc ccacctggag
     3001 gaacgtcgct cctgcctgtc cccggcgctc cggtgtcgcc ggatcatata caccatcttt
     3061 gatgtattga gtggtttgag ccacaccgga cgaagccgca aacagggccg cacacacggc
     3121 gccacgcagg caaaatcctc tgttcatggt atctcccgag atcgacatcc tgagcaactg
     3181 ccgcacgtca acgaattacg acgaacttgc cgattttcgt ccccaaggtt gcatgttcaa
     3241 ccacatagaa atacacgtcg ctgactatgt actggttgtc gctgttacgc ccatcccacg
     3301 ccggcaacag atcgcccgcg ttgaactgca gcgttttcac caaattcgca tccgacgaat
     3361 aaatcttgac cgtacacggc aacgggagat tcgcgaaccg cagccgctcg tacccgaaaa
     3421 tattcgaccg agacgcatac gttcttcccg aaacgttgta cgggttggga actactttca
     3481 cgctgtccag agtggtcgtc tgcgaaatgg cgtccgacgc gaacacacag aggaacgcga
     3541 ccaaaatggc tgtcggcaga gcccatcgac gggaaaatag agccattgcc catccctttc
     3601 agacaggcgt gggaggcacc agcctcccac gcccgccgaa tcgctgctta acgaacaagc
     3661 accatgcggc gaaccatttc gtttgtgccg ctggtcaagc gatagaggta cactccactt
     3721 gcaaccggac gacccatgtt gtcgttaccg tcccacacaa cgctgtggac accggcagcg
     3781 gtctgcccat tgatcagcgt gcgaaccacc tgaccggtaa ggctgtagac aacaagtttc
     3841 gtctgggcgg aacctgcaag gctgaacttg atggtcgtag tcgggttgaa cgggttcggg
     3901 gtgttctggg cgagcatgaa cttgcccgga gctgcagatg cgtccacgcc tacatattcc
     3961 ggcgcggtag cacccttgtt catgtacccg gaccacgtcc accagcgcgt gctctccatc
     4021 gagtacccgg ggagctccca gttctgggtg ccgtcgtcat acgcgacgac cgcgtaccag
     4081 tagtccacac cgggggtcac gttgacatcg tggaacacac gacccgccgg gaacgcacca
     4141 tcggcagcca cttccgcgaa gtcggacgca ggcccctgtt tgatcagcac catcggagac
     4201 atacgcgtcc ccgtctggcg atagatccgg taccccgcaa agtccatgac tttggtatcg
     4261 tggtcgggag ttgtttctgc ttccttaccc cagcggactt cgattcccac gcggttcaac
     4321 ggacgcaccg agaggaatcc aggcggacga ggagcatccg gagcgtcgaa cggcccgtat
     4381 tgcgccggct tcgacgtaac aaaccccgtc aacccccact tcgccaggtc agccgcagtc
     4441 acgttggcgg gcatgtggcc gtgaaagttc cagtacgaaa gcgcgcaggc tttcatcgcc
     4501 gtatcgcgtc ccgtctgaat aagcgcgata tccgccgccg acatccagct cgccgggtcc
     4561 ccggggttgg ccagcttgtt ggtattccag gcgttgccta tgcgccgcgc ttcttcaacg
     4621 tcaataccac ctgcacccat cacttcgaca aaccgcagag aatcgagcca cggcatgtca
     4681 tacccctgag aacgcacatt cgcgaggttc agcggccccg ccaatttgcc ataaccccag
     4741 attgcggagg caatgttcgc gccgccaacg taattgacga agcgaggatc gtcccggaaa
     4801 ccgacgttca agtccatctg gagagaaccg tcggcaatca tttccctggc ctgcgccgga
     4861 tctgccgggg agtaggtctt gccgaagtca aacccgtact cctgccagac ggtgcggaac
     4921 gcgggctgtg ccgggtcatc cacgccgtaa ttggcgccgg gccccgcact cgcgaacacc
     4981 ggaccattca ctatgtatgc gttgccggcc aggtggcctt caaagaacgc ggtctcgatc
     5041 gggtcacccc agtcgggtcc gggtgaagtc gggtcgtcga ggtcaacgtc ccacgccatc
     5101 ataatgctat ggttgcccgc gccgaacggt tcgacataca tcatttcctg gtcgccgcac
     5161 tgctggttgg caacgctggc catctcgtcc ttcagatgcc gtgtgtgcca ccagagcatc
     5221 ttgttgatga cctggttctg gatggttgca ccgtcggatg gggggtgatc gttcgccgta
     5281 cggccgctga taccattgtt taccagtttg tagtcggtga agacgtagtc ctggtgcggg
     5341 ctcgtctggc catacccgta gacgtcacga atgctttcga cacccatgat gtaccgccat
     5401 acggtgttga tcttcaattc cgtgacgaga ttcggatcga cgatcacatc aggacgtggg
     5461 cctttcccgc cgctcgccgg atacttgaac tggtccagcc cggcgcttcc ggggaagaag
     5521 atgaaatcaa tcagcgtgtc gcccgttgcc gacgtcggtg acttgaccac cagaatcagc
     5581 ttcggccgcg ggaaccgcat atactcttga atggataccg cgtacagata gttgaacgcg
     5641 tcgggtttgt cgaggcggta gcgcggtggt gatgacgtgt cgttgccgcg gtacatgtag
     5701 ctgtacgcat ccgcatacag cgtgccgagt tcgttcgtcc agtcgatcgt gagcatggaa
     5761 ccgccgtgct tgcgagtgtg cgtaagccac gaccgcttca gttgctgccc cgagccatcg
     5821 ctccactcgt ttcgcggaac gccgcccggg tagcacatga gtgagctttc cgcgggctcg
     5881 ttttccgggt tcgtccagaa tctgccgaga tggaattgcg cctgcaggcc attcgcgaac
     5941 acggccggaa ccgcaaccat cccgagcact ccggcgaaca ggactccttt tcgccagttc
     6001 attcctgaaa ctccttttct cctcacgagt gaggaaccct caaccagcgc aattccacag
     6061 acgtggagtt gcatgaaaac cacgtgctcc cgctaccagc acacctcctt tcaatcgtcg
     6121 agacatccaa cgtgcccatg agaagcatta caaatcgaaa cgaacgccca cccggtacga
     6181 gcgcgggtag agatgatgca tccattcggt ctgcgcgctc gtcgggcgct tccagttgcc
     6241 gtccttgtcc ttccaggggc gcgtgaacat cgcaaaggtg gactcatcgc cgaccttcat
     6301 gtcattcccc tgcagacgct gtccgtaaat gtagccgaga tagtcgatgt agttctggat
     6361 ccctccgcca ttgaggcgtt tgatgttgaa cacgttcccg atgtcgaggt acgcggacaa
     6421 tgatctgccg cggccaagct ggaacgcctt gctcagcttc aggtccgtgt tgtagctgct
     6481 tgccgaggga aggatgtttt cgtccggaat ctcccgacgt ggcaacgctt cggggttgta
     6541 gattacctcg cccccggagg cgtacgactg aacgacacta accgcccacc cgccgcggat
     6601 gttgccccag tccatcggcg ttcccacgcg aagcattccc tggaaatacc ctttcgggtc
     6661 tgaagtcgac gcgaaggatt caaacggctg gatgttgacg gtattcagat ccacaacggt
     6721 gaggccgacc atgccgctcc ttgaactggt tttctggtac gtaagccaac cgttgacaaa
     6781 ccgcccgctg cttcgggaaa tcttgatttc cgcgccctga atgtcttcgt agttggcatt
     6841 cctgaagatt gaatacccgc gaaaatccga tcgcagtgtg ttggtgacgg aaatacgcgt
     6901 cacctggtcc acgttgtact tggcgtaccc acggaccgtc atcaccagcc ccagcgggaa
     6961 gacgtgttcc acgccgactt cgtacatcgt ggtcttcgcg ggtcgcaggt tggggttgaa
     7021 catgtcggcg acccagccgt tgaatgccgc tcggccgtcg tggtcggagt ggccgtacat
     7081 cacggccgct ttctgcatcg agtagaagat gccgtagttg aagaagaact tcgtgcggta
     7141 gctaaccggg tgagatatgc cgaagcgcgg cgcaatccgc cagtgaacga tgttatccga
     7201 ttgcggaaaa atgcgcacaa cgtcccaggg ctccggggcg acgccaagtt tctccttcac
     7261 cttgaaatag tcggaaacgt tgttcaccgg aaccggcccg tacgcgagcg tgtcccaccc
     7321 gagggctacg gagaggtctt tgaaatagcg ggttccgtgg ttcacgaaga ggtcgttatc
     7381 gaacatgtcg ttgtagaaat atgcgttctg cccggcatcg aaccgttcga tccgaatacc
     7441 tccgttggcg atcatcccgt tcgtttcgaa cttgtcctgc agatacacgc ccaagatgct
     7501 cggattaact gccgggtagt tgccgccgta atcccgccac tctgtgttcg tggtcagagc
     7561 gctgttctga atcatgtggt attcaaggtc accacggata aactcgacgc ccgttttcag
     7621 cgtgtgagaa cccatcgcat gcgtgaaatc gctccggaac actatctggt ttgagcgccc
     7681 ggtcagccgc acgtggccgc cgccgcctat tccgtacact ccactcaggt ccttgtacgc
     7741 gctcatcaca tacccttgag gcgacacgac ccggcccact gtcgtggtgt cacctccgat
     7801 ttcccgatag atgaacttcg cttccgaagg gacctggtag tacaggtagg gaatgccgaa
     7861 cgggcttact ggcgccagac gattgggatt cagggcatcg gccaggctgc tgggtttatc
     7921 cgggccgccc accccgttcg tgtcagtcca tccgaatgcg aagcccatcc agccctgacc
     7981 gctccacgtg ttgggtggct gatagcgccc ccccggagca aagtcactga agtccgcgcg
     8041 cggaagatca cggttcactt cccatttcga cgaagatcgg cccagcgagg cactgaggaa
     8101 tgtcttgggg ctgaacgtgt aggtgaaggt cccgccgtat tgggagtact cgccattcag
     8161 aagcgagttg tacgagaggt tgaacttggt gttgtcgctt gtgccggtca gaccattgag
     8221 cagatcgccg ctcgccctca acatcaccgg atcgtttccg gttacgctgg ctccgcttgc
     8281 cgccagctca gggttcgatg ccgctccgcc ggtagaaccg gcagcagttc cctcgttccg
     8341 accggtcatg taactgaact gaattttgat tttgtccaat ggcgtgagcg tgaatttcgc
     8401 ttcaaatgtc tggtcctcgt tgtagggcct cagcgcggga accaccgtca tctcgtacgt
     8461 gcgggagtag ccaacaacaa cgccacccat ccttttcgga agcgcccacc ccgccgccag
     8521 atccaggttg taatcaggat tgttgctgta cttccacacc tgttcgttca tgttggcttc
     8581 ccattcccag gcctctcgga cctcggcggg agtccagtct cgtttaccga accagccgcg
     8641 cgcggcattc acaaccgtcg cgcgttgctt ccagccatcg aatacgcgga acgcgcttgt
     8701 gcgcgggttc acgtacttcg gattggtcgg atcggtgaat tccggtgccg tgcgcgtctc
     8761 ttcatagagc aagggccagt acagcgggcc tgccagagcg gaatcggcaa acggtgaatc
     8821 gctgctcatg agccagtaat catattgatc cgaatcgtac cctccgggac cgaaatgctt
     8881 cttcctcgcc ggggccatat tgtacacacc agacacccac ggcaatgcaa ctgccgcgct
     8941 ccggcgttcc gtgccgtcct tgttgaccac gttcaccata ccggaccgga cgtttccgta
     9001 ttccgcgttg aatccgccgg tgagaagcgt gacctcgctg acaagaacct ggttcaactg
     9061 ggtgtagggc ttgctggtca ggccgtccgt acggtccaga ccgtcaacct gaaaacggat
     9121 ttccgacggg cctcctccgc gaatctccag ttccgttccg cgcgtaacgc tcacgcccgg
     9181 ttcgtagtta aggaaatcca gcattcgcga tacaggcgct tccgaaatcc gtgcggcatt
     9241 cacaaccgtc tgcgaagacg tgacgtctac ttcgataggg gggcgctcgg cgcgaacca
//
LOCUS       DAOTNG010000105         9228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6861_length_9228_cov_4.895672, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000105 DAOTNG010000000
VERSION     DAOTNG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6861_length_9228_cov_4.895672"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="P5179_06570"
     CDS             <1..842
                     /locus_tag="P5179_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS94916.1"
                     /translation="TGSLNSAHPPVPGFVPPSTAKHYARVLVAEDNPLNQKLTQIMLE
                     KLGCEVVTVSNGVEVLSELASNSFDLILMDCQMPEMDGYETSRRIRESTVPRIREIPI
                     VALTASALIGDRDRCIEAGMNDFLPKPLQKSDLVATISRWASGDRSGANGRSGTGEVL
                     AFDPEKLMGRVGGNFHAARQLVEAFLRDARDHVALLGNAVSEGNASLLAAEAHAVKDN
                     ALHTCAPALASAAAALEAAGTQADFARYAELIEAVQRHHADAEREMCVFIGSEPKTEQ
                     TLI"
     gene            complement(916..2319)
                     /locus_tag="P5179_06575"
     CDS             complement(916..2319)
                     /locus_tag="P5179_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS94917.1"
                     /translation="MNLVLVILDTFRKDHVGAYGNPWIHTPHLDAIARESVLFTEAYP
                     ESLPTLPVRRALHTGQRVFPFHGHRPLKGDFVGAPGWGPINEEQDTLSELLSEQGYRT
                     AFITDTYHQFKPSKNFHRGFDEWVWIRGQESDKYLSGPPVLEAEIMARSTPSQKNDVW
                     FHRFMTSYLRNNTYRMTEKDYYPARLFSEASRWLWDNQDAERFFLTVDSFDPHEPWDP
                     PDHYRKLYDPDDDCANVIQSPYRRWKERLTERELRRIQANYAGECTLVDRWFGFFMET
                     LRTTGRLEDTLVVIVSDHGHNVGHDPGDKGLVSKQGHPMTKAVAQLVCMIRHPKGEGA
                     GTTCDKLIYNMDLSATMLALAGVEPKQKLDGINVWDAIRDTKKPLREYVTVAWGPLVT
                     VIDDTWWYNANIWGEGALLYRHREDPNLVTNLAEKEPAVCAMMRERAVQDAGGSIPEA
                     FKNYATIPGCTPYLGKD"
     gene            complement(2451..3380)
                     /locus_tag="P5179_06580"
     CDS             complement(2451..3380)
                     /locus_tag="P5179_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRS94918.1"
                     /translation="MNGTASQSPADRDAEIRKLRTLLRVSKIMNAESNRAKVIQRINE
                     EVREYVEADRFTVFFHDSDTDELYSYIASGLTYGEIRIPSTAGIAGAVFTSRETLRLA
                     DAYEDPRFNREIDKRTGYRTRSLLSMPIINQRGTCIGVVQALNKKTGNKEFSDEDVEF
                     LKELVEQISDLLDLLLHKEELMRRQAALQESLSRLAVYDYLLGEKTAMKVAMRWNRKL
                     HLWIGVAGIIGLTLMAITGIVVAHVRGRPYVTMLDLHTGKAFLPRELAFTYSDLTGVF
                     LAIVTLTGVVLWLYPVVTKWARRRLERKASARD"
     gene            complement(3446..3856)
                     /locus_tag="P5179_06585"
     CDS             complement(3446..3856)
                     /locus_tag="P5179_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94919.1"
                     /translation="MRTGAIVAIIASMGCALIACGNNNAEREAKLAAMSAQEREIQKV
                     LDQKCVKCHTAPDAKGNLDLTEPKNLLPLISSTQLFDDIALYNMLMGDSTIAEHAPKE
                     FQLQQSEVDMIRRWVLSEHSDLVPDSVRAQQQGT"
     gene            complement(3981..5102)
                     /locus_tag="P5179_06590"
     CDS             complement(3981..5102)
                     /locus_tag="P5179_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94920.1"
                     /translation="MPMTSHERLMAAYRKQEPDRVPVRVWAVDDRTQPIHPSYAPIVE
                     AAREKTDLVGGWGGGPRREMLCRVTHEERPSRIAEFEERVTTIHTPAGPLTRVDVFNR
                     SGKPGYCMKHLIESPEDAKRWLSIPFGPLQGEVSGFFERVREMGDRGVIMSGVSHPMY
                     AVQDRLGPEGFAFWSIDERPLLHEMIQRELEITLEYLRYILGRGVGPLFAYVGPEVCI
                     PPSQSVRDFEDFVVHYDRQWIELIHEFGGIVWCHCHGNTKAVLEGFIRAGNDVLQPLE
                     PPPWGDIELRDIKRQVGRRMALEGNLEKHEMYTSTPSEIRQRVRQAILDAGEGGGFVL
                     CPSAGLQEWPTCDEQTVANFLAYIDAGREYGKYPLSREW"
     gene            complement(5190..5315)
                     /locus_tag="P5179_06595"
     CDS             complement(5190..5315)
                     /locus_tag="P5179_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94921.1"
                     /translation="MKKLLLLLALVGAAVALVVWYRQTSASLDMVPEDAEDIELA"
     gene            5571..6662
                     /locus_tag="P5179_06600"
     CDS             5571..6662
                     /locus_tag="P5179_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS94922.1"
                     /translation="MSADIRGEWPNYGGALDPTVWMRIRASMNHEEGDRVPIWDYLDN
                     RPTFEYFHRPGDTHDETMRRAYHELGIDFCRGYGSSFSREDEGRAGDHEEWKISGLTQ
                     WATKKPISNYAELRSFQPRLFTEEDCSRWLKHTQEAQARMAPLSYFVPGVGTGFHEVY
                     DLMGQQLFSYAIYDERRELDRLFEAYGYNSTLIARHAASTKPGPLFFTWADIAYKGRL
                     LFSRSFLRETFIPMLRSVCEPLAEAGIKVVFHSDGDVTEILDDMLDAGINGLNPVEPQ
                     AGMDIGRLKKRYGKRLILVGNIDCSQVLPLGSVQDVVEAVKTCLREAGHGGGLFIGSS
                     SEIVPSTPLENVLAFFEACRTFGRYPLAV"
     gene            7210..>9228
                     /locus_tag="P5179_06605"
     CDS             7210..>9228
                     /locus_tag="P5179_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS94923.1"
                     /translation="MQRQRYVWLAGLVVSLMILVAGTAHAVTAGKIIGKITDAQTNEP
                     VLGAAVTVEGLRMGATTDADGRYFILNVPAGRYTLKVQMIGYAPVTQLDVLVNNDLTT
                     RADFRLRYQAVEVGEVTVVAERPLIEKTLTGTRRTTLREQLELMPVITSDDIYRAAPG
                     IVFDPIGGPVQQVTDGTIRVEDETRSGNTANPGYYIRGSRPGEVNYYLDNFPVSGVLA
                     LPGKAIEETNLLTGGFSAQYGNGTSIINTVTPTPGAAYTASLNWESDLLPGLVSERYN
                     YGTNVVTASFGGAVPGTNNRLRVWVLGDGNMTDDWDPRLSTQDANFIENGIREFTQRA
                     NMVDTLRTFWATLKPWDVFEGDRLILPSHKQDMYTFMGKAMYNVGAFQFTLSGFAWRR
                     QYQRFSPNGLFMDWDKNNFRNMPYPTTMQQATLLHRNLVAFGVRWALSPRTIVDAKLS
                     FSTTATTTGSLDLKSGSFGLFNDFDYSWHEYAPSWSPFYVDPADWATTNRAKYTQQRR
                     EVYYPLGVTNLFFTSGNSRSANAVAGNNVVGQLHATSQINNNHELSGGVEITRSMTKQ
                     DRLSDPWSGTPFFDQWGPFNPIYGAAFLQDKIEYEGLTVTAGVRMDYYDPDAYVWSDP
                     LKPGAGSIMPDGSINVNGRPDVNADKVNAFRDKRVDPKMTLSPRLGISH"
BASE COUNT         1851 a         2753 c         2715 g         1909 t
ORIGIN      
        1 tcacaggttc tttgaactca gcgcacccgc ccgttcccgg atttgtgccc ccgtccaccg
       61 cgaagcacta cgcacgcgtg ctcgttgcgg aagacaaccc gctgaaccag aaactgactc
      121 agatcatgtt ggaaaaactc ggttgtgagg tcgtaaccgt gtcaaacggt gtcgaagtgc
      181 tctccgaact cgcctccaac tcgttcgacc tgatcctgat ggattgccag atgccggaga
      241 tggacggcta cgagacttcg cgcaggattc gggagtctac cgtcccgagg atccgcgaga
      301 ttccgatcgt cgcgcttacc gccagcgccc tcattggcga ccgcgaccgg tgtattgaag
      361 ccggtatgaa cgacttcctg cccaagccac tgcagaaaag cgacctagta gctacgatct
      421 cacggtgggc ttcgggggat cgatccggcg caaatggcag atcagggacc ggagaggtcc
      481 tcgcgttcga tccagagaaa cttatgggcc gtgtgggagg taactttcac gcggcgcgcc
      541 agctagtgga agcgtttttg cgagatgcac gcgatcatgt ggcattgctg ggcaacgcgg
      601 tttcagaagg gaatgcgtcg ctgctggcgg cggaagcgca cgccgtcaag gataacgcgc
      661 tgcacacgtg cgcgcctgca cttgcgagtg cggcggctgc tcttgaagcg gcgggcactc
      721 aggcggattt cgcgcggtat gcggaactga ttgaggctgt tcagcggcat cacgcggacg
      781 cagaacgcga aatgtgcgtg ttcatcggtt cggaaccgaa aaccgagcaa acccttattt
      841 gaagcgcgcg ggcagtcacg cactacccac gcgccgctca gaacgctcat ctgccgttcg
      901 cgtgaggggc tcttttcaat ccttcccgag atacggcgtg caacccggta tcgtcgcgta
      961 gttcttgaac gcttccggaa tgcttccacc cgcgtcctgt accgcgcgtt cacgcatcat
     1021 ggcgcacacg gcaggctctt tttcggcgag gttggtcaca aggttcggat cctccctgtg
     1081 gcgatacagc aacgctccct ctccccagat gttcgcgttg taccaccagg tatcgtcgat
     1141 caccgtcacc agtgggcccc aggccaccgt aacgtattcg cgcaacggct tttttgtatc
     1201 acggatcgca tcccagacat tgatcccgtc cagcttctgc ttcggctcca ctcccgcaag
     1261 cgcaagcatg gttgcggata agtccatgtt gtagatgagc ttgtcgcatg tggttccggc
     1321 gccttcacct ttcgggtggc ggatcatgca cacgagctgg gcgaccgctt tggtcatcgg
     1381 gtgaccctgt ttgcttacaa ggcctttgtc gcccgggtca tgcccgacat tatgcccgtg
     1441 gtcgctgaca ataacgacca acgtgtcttc aagcctgccg gtagtgcgca gtgtttccat
     1501 gaagaaacca aaccaccggt ccacaagggt gcattcgccg gcatagttcg cctgaatacg
     1561 tctgagctcg cgttctgtca accgctcctt ccaccggcgg tagggagact ggatgacgtt
     1621 cgcgcagtcg tcatccgggt cgtacaactt ccggtaatgg tccggggggt cccacggctc
     1681 gtgcggatcg aacgagtcca ccgtgaggaa aaacctctcg gcgtcctgat tatcccagag
     1741 ccaccgtgat gcttcggaga aaagccgggc cgggtagtaa tccttctcgg tcatccggta
     1801 ggtgttgttg cggagatacg aggtcatgaa ccggtggaac catacgtcgt tcttctggct
     1861 cggcgtgctg cgggccatta tctctgcttc gagcaccgga ggtccgctca ggtacttgtc
     1921 ggattcctgt ccacggatcc acacccattc gtcaaagccg cggtggaagt ttttcgacgg
     1981 tttgaactga tgatacgtgt ccgtgatgaa cgccgtgcgg tatccctgtt cgctgagaag
     2041 ctctgaaagc gtgtcctgtt cctcattgat gggaccccac cccggcgccc cgacaaaatc
     2101 ccccttcagc ggccggtggc cgtggaacgg aaacacccgt tgtccggtat gcagcgctcg
     2161 ccgcacggga agcgtcggaa ggctctcggg atacgcctcg gtgaacaaca cactctcccg
     2221 cgcgattgcg tcaaggtggg gcgtgtggat ccacgggttg ccgtaggcgc cgacatggtc
     2281 tttcctgaac gtgtccagaa tgacaagcac gaggttcatg gttcggtttc ctccggtgtg
     2341 tggtggcaaa acgcgatatc cgcaaatatg gcggcggaga gagtccgccg caacgggaca
     2401 ccgggttacc cggagggtgg cgacaagggg aagtaaccgg gaattgcggc tcagtcccgc
     2461 gccgaggctt tccgctcaag gcggcggcgc gcccatttcg tcacgaccgg gtagagccac
     2521 agcacgacgc cggtgagtgt cacaatggcc agaaatacgc cggtaagatc gctgtaagtg
     2581 aacgcgagtt cccgagggag gaatgccttt ccggtgtgca gatccagcat cgtcacgtaa
     2641 ggacggcccc gcacatgggc gacgacgatg ccggtgattg ccatcaatgt cagtccgatg
     2701 atgccggcaa ccccgatcca aaggtggagc ttgcgattcc accgcatcgc caccttcata
     2761 gcggtcttct cgccgaggag gtagtcgtat accgcaagac gggaaaggga ttcctgcagt
     2821 gcggcctgcc gacgcataag ttcctccttg tgaagaagca ggtccagaag gtcgcttatc
     2881 tgctcaacca gttccttcag gaattccaca tcctcatcgg aaaattcctt gtttccagtc
     2941 ttcttgttca atgcctggac aactccgatg cacgtaccgc gctggttgat gatgggcatc
     3001 gacaggaggc ttctggtccg ataccccgtt cgtttgtcaa tctcgcggtt gaaacggggg
     3061 tcctcgtacg catccgccag ccgcagggtc tcgcgcgagg taaacactgc accggcgatc
     3121 ccggctgtgg agggtatcct gatctctccg tacgtgaggc cgctggcgat gtagctgtag
     3181 agctcgtcgg tatctgagtc gtggaagaac accgtgaagc gatccgcctc aacgtactcg
     3241 cgcacctcct cgttgatgcg ctgaatcacc ttggcgcggt tcgattcggc gttcatgatt
     3301 ttgctgacac gcagaagcgt tcgcagtttg cgtatctccg catcgcggtc agcgggagat
     3361 tggctggcgg ttccgttcac gcgcaactcc tgacgacaca gaaacgaaaa ggcgccctga
     3421 gagggcgcct ggaaccggga acgagtcagg ttccctgctg ttgtgcccgc acggaatcgg
     3481 gaacaaggtc gctgtgctcg ctcaacaccc agcgacgtat catatccact tctgactgct
     3541 gcaactggaa ctctttcggt gcatgttcgg cgattgtgct gtcgcccatc agcatgttgt
     3601 aaagggcgat atcgtcaaac agctgcgtac tcgagatgag cggcaggagg tttttgggtt
     3661 ccgtcagatc gaggttgccc tttgcgtccg gcgcagtgtg gcatttcacg catttctgat
     3721 ccagcacctt ctggatctcg cgttcctgcg cgctcatcgc cgcgagtttc gcctcgcgtt
     3781 ccgcattgtt gttgccgcac gcgatgagcg cgcagcccat actggcaata atggcgacaa
     3841 ttgcacctgt tcgcattggc gaaactcctc atgatggatg aaacggtcga actcccacgc
     3901 cgcatactcg caaagaaata taaatgggcc tgagcaaagt cccaagcgtg cgcggcgacg
     3961 cgcactctgc ccctgagcgg tcaccattcc cgcgaaagag ggtacttgcc gtactcacgg
     4021 cccgcatcga tgtacgcgag gaaattggct accgtttgct cgtcgcaggt aggccattcc
     4081 tggagcccgg cgctcgggca cagcacgaat ccaccacctt ctcccgcgtc cagaatggcc
     4141 tggcgaaccc gttgacgaat ctcggaaggg gttgacgtgt acatctcgtg tttctcgaga
     4201 ttcccttcca acgccatcct gcgtcctacc tgacgcttga tgtctctcag ctcgatatcc
     4261 ccccaggggg gaggttcgag cggctggaga acatcgttgc ccgcgcgaat gaagccctca
     4321 agcactgctt tcgtattccc gtggcaatgg caccatacga tgcccccgaa ctcatggatc
     4381 agttcgatcc actgccggtc atagtgcacg acgaaatcct cgaagtcccg aacagactgg
     4441 cttggcggaa tgcacacttc gggccccaca tacgcgaaca gtggccccac tccgcgcccc
     4501 agtatgtagc gcaggtactc cagcgttatt tcgagttccc gctggatcat ttcgtgcagg
     4561 agagggcgct cgtcaatact ccagaacgcg aacccctcag ggccgagccg gtcctgaacc
     4621 gcatacatgg gatgggaaac accggacatg atgacaccgc ggtcacccat ctcccgaact
     4681 cgctcgaaaa agccgctgac ttcgccctgg aggggcccga acgggatgga cagccagcgt
     4741 ttcgcgtcct ccggggactc gatcaggtgc ttcatgcaat accccggttt cccggaccga
     4801 ttgaacacat ccactctggt gagcggcccg gctggggtgt ggatcgtggt tacccgttct
     4861 tcgaattccg cgatgcggct gggacgctcc tcatgcgtaa cgcggcacaa catctcccgg
     4921 cgggggccac ccccccaccc acccacaagg tcggtttttt cccgtgcggc ttctacgatg
     4981 ggagcgtacg aaggatggat cggttgcgtc cgatcatcca ctgcccacac ccgcaccggt
     5041 acccggtcag gttcctgctt tctgtacgct gccatgaggc gctcatgact cgtcatcggc
     5101 atcaggtggt tcctcgttga ggggtaccgc agtctgcaac aaaaaaggcg gggtcggaca
     5161 acgccccgcc ttgaatctca caccgctgct tacgcaagct cgatgtcctc ggcgtcttcc
     5221 ggaaccatgt ccagcgacgc gctggtttgc ctgtaccaca cgacaagcgc aaccgccgct
     5281 cctacaaggg ccagaagcaa gaggagcttc ttcatagcct tcctcccacg gaaaaggtcc
     5341 actgcccact ggataaaaaa tacggcaatt tgggagaaaa agacagccgt tgcgtccatt
     5401 tttcctgcac cggttgcggc aattcagcgg aaccgcggag cgctcctcgt tattccattt
     5461 tccgtgcttc cgcgcacctg ctcccagtgt cccttgagga ggggtcgctg gcttcgtttc
     5521 ttcctgactg tcaacgttcg agcacggccc ggcgaaagag gtgacgagtg atgagtgcgg
     5581 atattcgggg cgaatggcca aactacgggg gagctcttga cccaaccgtc tggatgcgga
     5641 ttcgggcctc catgaaccac gaggagggcg accgggtgcc catctgggac tacctcgaca
     5701 accggcccac gtttgagtac ttccacagac cgggcgatac gcatgacgaa accatgcgac
     5761 gcgcgtatca cgaactgggg atcgacttct gccggggata cgggagcagt ttctcgcgcg
     5821 aggacgaagg ccgggcgggt gaccatgaag agtggaaaat ctcagggctc acccagtggg
     5881 cgacgaaaaa accgatctcg aactatgccg aattgcggtc gttccagccg cggctcttta
     5941 cggaagaaga ctgttcccgc tggctgaagc acacgcagga agcccaggcg cggatggcgc
     6001 cgctttccta cttcgtcccc ggcgtgggaa cagggttcca cgaagtgtac gacctgatgg
     6061 gtcagcagct tttcagctat gcgatatacg acgagcgcag ggaactcgac cgtctctttg
     6121 aagcgtatgg ctacaattcg acgctgatag cgcgtcatgc cgcgtcgaca aaaccgggcc
     6181 ccctgttttt cacgtgggcg gatattgcgt acaaagggcg tctcctcttt tcccgttctt
     6241 tcctccgcga aacgttcatt ccgatgttgc gaagcgtctg cgaacccctt gcggaggcag
     6301 ggatcaaagt ggtgttccat tccgacggtg atgtcacgga aatccttgat gacatgcttg
     6361 acgcaggcat taacggcctg aatcctgtgg agccgcaggc cggaatggac atcgggcgcc
     6421 tgaagaaacg gtacggcaaa cgcctcatac tggtggggaa tatcgattgc tcgcaggtat
     6481 tgccgctggg cagcgttcag gacgtggtgg aagcagtcaa gacatgcctg cgggaggctg
     6541 gtcacggcgg ggggctgttt attggctctt caagtgagat cgtgccgtcc acccccttgg
     6601 aaaatgtgct ggcgtttttt gaggcctgcc gaaccttcgg gcgctacccg ttggccgtgt
     6661 aagcggagaa cgcctgccag acggttccat gtctgcgcgt cggttttgct cgggaaccgg
     6721 tctgataccc aagaatgcgt ggcggagaac gaagacagaa cacgcgagcg ggagaccggt
     6781 ttcccggccg tggaaatacg ccatccgtcc ttgaccccgc gcctgaaagc gactattttc
     6841 cctattgctt agacaggaga atcttttcag ctcccaccct ccggaatttc tgttcgtccg
     6901 tctggaaggg gcgttctggc gtacgcacgc tgccggtgca tgcgccgggt cgcttttttt
     6961 cgcgggcgcg gaagtcacgg gtggccgcga aacgtgtacg gtttcccgct cgctgttttc
     7021 ttggaacgcg gggatcgtgc gcgcagttcc atgcgcggct gcgtgctcag aagaacgggg
     7081 aggaaagatt ctcagcctga tgcacggttt cgtttcggta cgcgtgtatc cgttcgtccc
     7141 cgggatctgg acttgatgcc ggggaattca ccgaggtggg taaccaccgc atcactagga
     7201 ggcacctgaa tgcagcgaca gcgctatgtg tggctggcag gcctcgtcgt cagtctgatg
     7261 atcctcgttg cgggaaccgc gcacgcggtt accgcgggga agatcatcgg gaaaattacc
     7321 gatgcccaga cgaatgagcc agttctcgga gcggccgtga ccgtcgaggg cttgcgaatg
     7381 ggcgcaacga ctgacgccga cggacggtac ttcatcttga acgttccagc aggtcgttac
     7441 acgctgaagg tccagatgat cgggtatgcc ccggttaccc agctggatgt tctcgtgaac
     7501 aacgacctga ccacgcgggc agacttccgg ttgcggtacc aggcagtgga agtcggtgag
     7561 gtaacggttg tcgccgaacg acccctcatt gaaaaaacac tgacaggcac ccgccggacg
     7621 accctgcgcg aacagctcga gctgatgccg gtaattacct cggacgacat ctatcgtgcc
     7681 gcccccggta tcgtgtttga cccgatcggt ggaccggttc agcaggttac tgacggaacc
     7741 atccgtgtcg aagacgagac acgctccgga aacacggcaa accccgggta ctacatccgc
     7801 ggaagccgtc ccggcgaagt gaactactac ctcgataact tcccggtaag cggcgttctc
     7861 gccctccccg gaaaggcgat tgaggaaacc aacctcctta ccggtgggtt cagcgcgcag
     7921 tacggcaacg gaacctccat catcaacact gtgacaccca ccccgggtgc agcgtacacc
     7981 gcctcgttga actgggagag tgatcttctt cccggcctgg tttcggagcg ttataactac
     8041 ggaaccaacg tggttaccgc gagcttcggc ggcgcggttc cgggaaccaa caaccgcctg
     8101 cgcgtctggg tcctcggcga cgggaatatg accgacgact gggatccccg tctttccaca
     8161 caggacgcga acttcatcga aaatggtatc cgcgagttca cccaacgcgc caacatggtg
     8221 gacacgcttc ggacgttctg ggccacgctc aaaccgtggg acgtgtttga aggtgatcgc
     8281 ctcatcctgc catcgcacaa gcaggacatg tacacgttca tgggcaaggc gatgtacaac
     8341 gtgggcgcgt tccagtttac gttgagcggc tttgcgtggc ggcgtcagta ccagcgtttc
     8401 agccccaacg gcctgttcat ggactgggac aagaacaatt tccggaacat gccgtacccc
     8461 accaccatgc agcaggcaac gttactgcat cgcaatctcg ttgccttcgg tgttcggtgg
     8521 gcgctgagcc cgcgcaccat cgtcgacgcg aagctgagtt tctcgacgac cgccaccact
     8581 acgggttcgc tggacctgaa gagcggttca tttggtctgt tcaacgattt cgattactcc
     8641 tggcacgaat atgcgccgtc gtggtcgccc ttctatgtgg atccggcgga ctgggctacc
     8701 accaatcgcg cgaagtacac acagcaacgg cgcgaagtgt attacccgct tggcgtgacg
     8761 aacctgttct ttacgagcgg gaattcccgc agcgctaacg cggttgccgg aaacaacgtt
     8821 gtaggacagt tgcacgctac ttcacagatc aataacaacc acgaactgag tggtggtgtc
     8881 gaaatcacgc gcagcatgac caagcaggac cgcctgagcg atccgtggtc gggcaccccg
     8941 ttctttgacc agtggggtcc gttcaatccg atctacggag cggccttcct gcaggacaag
     9001 atcgaatacg aaggtctcac ggttacggcc ggtgtgcgca tggactacta cgatccggat
     9061 gcgtacgtct ggtctgatcc gctgaaaccc ggtgcgggaa gcatcatgcc ggacggcagc
     9121 atcaacgtca acgggcggcc tgacgtgaac gcggacaaag tgaacgcctt ccgcgataag
     9181 cgcgtagacc cgaagatgac gctcagccct cgtctgggca tcagccat
//
LOCUS       DAOTNG010000106         9187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6890_length_9187_cov_4.965615, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000106 DAOTNG010000000
VERSION     DAOTNG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6890_length_9187_cov_4.965615"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..900)
                     /locus_tag="P5179_06610"
     CDS             complement(100..900)
                     /locus_tag="P5179_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HRS94924.1"
                     /translation="MLVVGERINSTRKRLLPAIETRDIAVITDEATMQVNAGAHYLDC
                     NAAAVGVEKEPEYLTWLVATVQQVSEDTPCAIDSPNPVAVEAALKVHKGVPMINSISA
                     EKAKYDGLMPLARDSKAKVVALLMDDAGIPKTAADRIAIGRSLVTSLVKDGIAADNIY
                     VDPLIYPIGAEASAGTAVLETISTLKKEFDGVHFICGLSNISYGLPVRKLLNQAFMVL
                     TIGAGLDAAIIDPTDRKLMSLAFAAEALVGADEYCANYIQAARQERLE"
     gene            complement(940..1959)
                     /locus_tag="P5179_06615"
     CDS             complement(940..1959)
                     /locus_tag="P5179_06615"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRS94925.1"
                     /translation="MDNKASDSQVSPVSRPCASTLERVLRDKKFVVTAEIVPPRVPNL
                     SVVGKKFELLREVADAINITDNASASVKICSLAVCAYLVQQGGEPVFQVTARDRNRLA
                     LQSDLIGAYALGVRNVFAVTGDYINMGDHPAGKPVYDMDSVHIIQMFDKIRHGYMDSG
                     VELRSTSKTPLVQPNFFLGAAANPFGDPMPFHVLKVAKKRLAGADFIQTQCTFDVPRV
                     REFMAMVVDKGFHERLHFLIGIMPVKSARPLEFMSTEVPGMRVPAEAIARMKGAQNAE
                     EEGIQMAVETIQQVREIPGVSGIHLMTVSWEAVVPEVLKRAGLMPEQRGVVEIHAAAK
                     SGIAS"
     gene            complement(2038..2445)
                     /locus_tag="P5179_06620"
     CDS             complement(2038..2445)
                     /locus_tag="P5179_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRS94926.1"
                     /translation="MFFNVKAKPWYIRLVDIGEQFAKVPLFDCSMCGQCVLRSTGLTC
                     PMRCPKQMRNGPCGGSMNGKCEVFPERPCIWNLIWERSEMLGWEEKLENINPPVDWRL
                     WGTPSWWNLLTGRIDGKGHAVEPKKESEPSKTN"
     gene            complement(2666..3304)
                     /locus_tag="P5179_06625"
     CDS             complement(2666..3304)
                     /locus_tag="P5179_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HRS94927.1"
                     /translation="MAQLQALADAIIAGNRVKTAELTAEALSAGMPAKQILDEGLIAG
                     MAVVGEKFKNCEFYVPEVLVAARAMAAAMEKLRPALVEAKVEPIGKVAIGTVRGDLHD
                     IGKNLVAMMLEGAGFEIIDLGVDCSPEQFVGAVKEKGANIVALSALLTTTMPAMRDTI
                     EAMTAAGVRDKAKVIIGGAPVTQKFADQIGADGYAADAASGVDVAKSVLGIA"
     gene            complement(3645..4058)
                     /locus_tag="P5179_06630"
     CDS             complement(3645..4058)
                     /locus_tag="P5179_06630"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRS94928.1"
                     /translation="MRITGYYSFESAHRLHDAGCSNEENAALYGPCQTIHGHTYRLEV
                     TLEGSKLEHGMFLNFERVDEIVRDHVIKRLDHHMIDDEPYFRSHPSTAEELARWIWGQ
                     IEPHFARLAGCRLAEVKVFEGEHFSACVDRSDFEH"
     gene            complement(4124..5104)
                     /locus_tag="P5179_06635"
     CDS             complement(4124..5104)
                     /locus_tag="P5179_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94929.1"
                     /translation="MDLTSRERFVATFAGTSRCSPPLWGDGIRDDVVEDWASQGFRPD
                     AGWERTLGIERFEVVQPDLRHVDFSFTPVTDPEPVQTCPADDIRRYPRDWDQQVVSFS
                     TRDFAVGLRVSRGLFLTFGVADWNSLAPLLIALHDEPETMSHRLREAAEFAMQVLQRA
                     YDSVKFDFVVFSEPIASNCAPVISPEMLREVCGAAYKVLLDQARKAGVSHVVFQTYGN
                     TASLIPAFRDLGASIFWSGESGVAGVSYPRIRNRYGGTLGLIGGIDSAILSLNENEME
                     RRLELDVVPLLEQGRYLPLLDGRVRRGISYQSYAKYRHLLKDVVARACGP"
     gene            complement(5095..5691)
                     /locus_tag="P5179_06640"
     CDS             complement(5095..5691)
                     /locus_tag="P5179_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94930.1"
                     /translation="MVDETKASAGGAVVKTLDDLPGVGPTKAKWLKAAGIKDLQAARD
                     VPLEKLMHVRGIGRLLAVRIKEVLTGQEIGSDATASTDAQASDLTEGQMQWQETVTEH
                     QGSIMVLVSDLLAQPQKYGLKPKFMKDLEKLGAVVDALPVSTPPIDGTRRGKIVKHLK
                     AIQVLLESAVKMDEDSDKHRKVVKEKLRDRRKKLERWT"
     gene            complement(5714..6439)
                     /locus_tag="P5179_06645"
     CDS             complement(5714..6439)
                     /locus_tag="P5179_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HRS94931.1"
                     /translation="MAKRRFMGTGLPYVDLSEVNGHLLVVEGSDGVGRTTQLSLLRTW
                     LEVQGYGVIETGWTRSALVRESIDLAKAGNALNLLTFNLLYATDFADRLEHEIIPALR
                     SGFVVLADRYVYTAFARALARGTDPEWIRRLYGIAIEPDLVVYLRVDVNTLIHRVLLA
                     DSLDHWEAGLDQYPQLDPYESFIKYQSDVIREFDKMSDEYGFTVIDARKKVDEIQREL
                     RAAISATLSQSAGADVVAPTVDE"
     gene            complement(6455..7210)
                     /gene="tmk"
                     /locus_tag="P5179_06650"
     CDS             complement(6455..7210)
                     /gene="tmk"
                     /locus_tag="P5179_06650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRS94932.1"
                     /translation="MARLHEKHTFPGRLIIVEGIDGSGKTTQASLLRQWLVSLGVPVF
                     WTEWNSSALVKSSTKKAKKDNTLTPTTFSLLHVTDFADRLSYKIIPPLQAGMVVIADR
                     YAFTAFARDTARGVHPEWVRSLYDFAIKPDLAVYFKVPIRVSLRRLLSARMKLKFYEA
                     GMDTGLSKDPVECFELFQSRVLDEYDRMSEEFGFHVIDAVQPIEAQQKQFRALVSREL
                     QSYLAPYLAAKEQKTGNESKDSTVRRAGTQNDG"
     gene            complement(7194..7883)
                     /locus_tag="P5179_06655"
     CDS             complement(7194..7883)
                     /locus_tag="P5179_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS94933.1"
                     /translation="MSGLLSPLRMGEKGLESASIARSARKAAVIELARSWDFEEAHAV
                     QVTKLALELYGGLPPPKKNREWEREWLEYASLLHDIGYINGVRRHHKVSEAMICRADL
                     IGFRRSEKQIIAAVARAHRKRFPDADDPVFDGLGDDDRESVRRLAGVLRIADGFDRTH
                     RGLITSAQMRVSQNDCTISCKAVGGVDEELRAAGRKSDVLQELLGVPVKFIVVSSGGF
                     PEREGENGTTP"
     gene            complement(7849..>9187)
                     /locus_tag="P5179_06660"
     CDS             complement(7849..>9187)
                     /locus_tag="P5179_06660"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRS94934.1"
                     /translation="VGFYEDPENHHLRGRDIVANEGLRGFTPLNLKPHELPTDHTHMV
                     ACIVAFHRKQVVPEAEPLWMELPPDLQETTLRLAAIVRIADGLDSNMEQTTRVIAVKG
                     RRRPVVGVAGNQDTLDHDVARAEKKADLWESCIGAPPQIVAARRRSPWRPPIRRKDSV
                     GESACIILRGYLTQVTAAIPGIGSILSVKPRHDMRIALRRIRAGLRLYRFIWEETAYN
                     ELSRELVWFGGLLGNVRDLDITILWLDKMMTACPDDVKKGLLRLRENAARRRREKLRR
                     LLAGLRSARFSALLIRLDDWVARGTGAVHILPGAEEVLGDEIRKVLARRARGILRYVG
                     TVKESSSERQHALRRECRRMRYAVDAWYRVLGKDRSDVLRALVNVQDALGDVHDADVR
                     LSVWRESERNVAVKWLRKRCQLERMKAWKAFKNTWPELQKKLDERVIESLANG"
BASE COUNT         1886 a         2902 c         2379 g         2020 t
ORIGIN      
        1 gttccccgcg ggtgcgaaat cagacctttc ctgagagatt tccggcgcag ggcgcgcacg
       61 tatcgaactt gcgaaccctg caaaccgaac gggaagaccc tattccagac gctcctggcg
      121 tgctgcctgg atgtaattgg cgcagtattc gtctgcaccg acgagggcct cggcggcaaa
      181 cgccagcgac atcagtttgc ggtctgtcgg gtcaatgatc gctgcgtcaa gcccggcgcc
      241 gatggtaagc accatgaacg cctgattgag caatttgcga actggaagac cgtaggagat
      301 gttgctcaat ccgcagatga aatggactcc atcaaactcc ttcttcagcg tgctgatggt
      361 ttccagcacc gctgttcctg cgcttgcctc cgcaccgatg gggtaaatga gcgggtcaac
      421 gtagatattg tctgcggcaa taccgtcttt cacgaggctg gtgaccaatg atctgccgat
      481 cgcgatcctg tccgcggccg ttttcggtat tcctgcgtcg tccatcagca gagccacgac
      541 cttggctttt gaatcgcgcg ccagtggcat caacccgtcg tacttcgcct tttcggcgct
      601 gatagagttg atcatcggca cccccttgtg taccttgagc gccgcctcca ccgcaactgg
      661 gttcgggctg tcgatggcgc agggcgtgtc ctccgacacc tgttgaaccg tggcgaccag
      721 ccacgtaagg tattccggct ccttctccac gcccacggcc gctgcgttgc aatccaggta
      781 gtgcgcaccc gcattgacct gcatcgtcgc ttcgtctgtg ataactgcga tatcccgcgt
      841 ttcgattgct ggcagaaggc gtttgcgtgt gctgttgatg cgttctccga caactagcat
      901 cgttgtcctc tcctgtcttt tctgtgtgtt ccagtgtatt caggaagcaa ttcctgactt
      961 cgcagcagcg tgaatctcta ccacgccgcg ttgctcaggc atgagacccg cacgtttcaa
     1021 tacttccgga acgacggctt cccaactcac ggtcatcaag tgaataccgg aaacgccggg
     1081 tatttcgcgc acctgttgga tggtttccac ggccatttga atgccttctt cctccgcgtt
     1141 ctgcgcccct ttcatgcggg ctatcgcttc cgccggaacc ctcattccag gcacctcggt
     1201 gctcatgaat tccaagggac gcgcactctt cacaggcatg attccgatca ggaaatgaag
     1261 ccgctcgtgg aatcctttgt ccaccaccat cgccatgaat tcccggaccc gcgggacatc
     1321 aaacgtgcac tgcgtctgga tgaaatcggc ccccgcgagg cgcttttttg caactttgag
     1381 aacgtggaat ggcatcggat cgccaaacgg gttcgcggcg gctccaagaa aaaagttggg
     1441 ttgcacgagg ggtgttttcg aagtggaacg aagttccaca ccggaatcca tgtacccatg
     1501 ccgaattttg tcaaacatct ggataatgtg cacggaatcc atgtcgtaca cgggtttgcc
     1561 ggccgggtga tctcccatgt tgatgtagtc ccccgtcact gcaaacacgt tccggacgcc
     1621 cagggcgtac gcgccaatca gatcagactg cagcgccagc ctgttgcggt cccgcgcggt
     1681 cacctggaac accggttcgc ccccttgctg caccaggtac gcacagaccg caaggctgca
     1741 gatcttgacg gaggccgacg cgttgtcggt gatgttgatc gcatcagcga cctcacgaag
     1801 cagctcaaat ttcttgccaa ccaccgacag attcggcaca cgaggcggaa caatctccgc
     1861 tgtgacgaca aacttcttat cccgcagtac tcgctcgagc gtgctcgcgc atggccggga
     1921 aacgggggag acctgtgaat ctgatgcttt gttatccatg gttcgggaag ctcctgggaa
     1981 tcggaacgaa cgccggcata cggaaactgg tgtcaggggt tatgtcgttg ttccgcctta
     2041 attcgttttt gagggttctg attccttttt cggttccact gcatgtccct tcccgtcgat
     2101 cctgccggtc agcaggttcc accacgacgg agttccccac agtcgccagt ctacaggtgg
     2161 gttgatgttt tccagttttt cttcccatcc cagcatctcg gagcgctccc agatgaggtt
     2221 ccagatgcac ggccgttcgg gaaatacttc gcacttgccg ttcattgaac cgccgcaggg
     2281 cccgtttcgc atctgcttcg ggcaacgcat ggggcacgtc agtccggtgg atctcaaaac
     2341 gcactgcccg cacatggaac aatcgaacag cggaaccttg gcaaactgct cgccaatatc
     2401 cacaagacgg atgtaccacg gtttcgcttt tacgttgaaa aacatcgtct accctctctt
     2461 ttcgaacccg ctcgttcgtc cgttactgat ttcccgggat tacgcactct ctgccgttgc
     2521 tggcaattga acgaagcatg cgccgccaaa gggaaggaaa accctgcagg tgttcgcgta
     2581 caaaagcgcc gactgggaac acacgcaccc agccggcgtt ttcgcttgcg atccggtttg
     2641 tcggcaaatc tgcgcggaac tggaactacg ctattccgag cacgctcttg gcaacgtcca
     2701 cgccgctcgc cgcgtccgca gcataaccgt ccgcgccaat ctgatcggcg aacttctgtg
     2761 ttaccggagc gccaccgata atcacctttg ccttgtcccg cacgccggcg gccgtcatgg
     2821 cttcgatggt gtcacgcatc gcaggcatcg ttgtcgtgag cagagccgaa agggcaacta
     2881 tgttggcacc cttttctttc acggcaccga caaactgctc gggcgaacaa tcaacgccga
     2941 ggtcgataat ctcaaaaccg gcaccctcaa gcatcattgc cacgaggttc ttgccgatgt
     3001 catgcaggtc gccacggacg gtaccgatcg caactttgcc tatcggttcc actttggctt
     3061 ccacaagcgc aggccgaagc ttttccatcg ccgccgccat agcccgcgcg gcgaccaaca
     3121 cctcgggaac gtagaactcg cagtttttga acttctcgcc aaccacggcc atacccgcaa
     3181 tcagcccctc gtcaagaatc tgcttcgcag gcattcctgc gctcaacgct tccgcggtga
     3241 gttcggccgt tttgacgcgg ttgcccgcga taatcgcatc agcaagcgct tgaagttgag
     3301 ccatgttcgt gcctccgtcg tttcggcttg aaatcagcac gcgcccgtcc acgcaaacga
     3361 acgcgccctg gattgttcgt ttcaaaaacg ggaactgccc tgtcagacac tcccggcacc
     3421 cttcggcagt ctccggactc cccggaaacc cggaataagt gctgcctgca aactcgaata
     3481 ttgtacgaac gtcaatggga cccgaaaacg caacaccgcc cctcaacggt tgtaaaaaaa
     3541 acaggaccca cgtgcagaac gcaagggtgc gatgcaacca caatcactct gatcccctcg
     3601 caccatcaac gcgtttcgta acgattcgtc ggggctcggg tcgctcaatg ctcaaaatcc
     3661 gatctatcaa cacatgcgct gaagtgttcc ccttcgaaca ccttcacctc cgcgagcctg
     3721 caaccggcga ggcgtgcgaa gtgcggttca atctgccccc agatccacct cgcaagttcc
     3781 tcagccgtac tcgggtggct ccgaaagtaa ggttcgtcat caatcatgtg atggtccaaa
     3841 cgcttaatca catgatccct cacaatttcg tcaactcttt cgaagttgag aaacatgccg
     3901 tgttccagct tcgacccttc aagagtcact tcgaggcggt acgtatgacc gtggattgtc
     3961 tggcaggggc cgtagagcgc agcgttttcc tcattggaac aaccggcgtc gtgcagacga
     4021 tgtgcagact cgaacgaata gtaccctgtg atacgcatct ctcggtatcc tgatgaaaca
     4081 cgggataacc cgtcgtcagc cgtttgggta gcccgtcata aaatcacggc ccacacgccc
     4141 tggcaactac gtccttgagc agatgccggt attttgcgta agactgatag gagatgccgc
     4201 gccggacacg gccatccaac agaggaagat accttccctg ctcaagaagc ggcaccacat
     4261 cgagctcaag ccggcgctcc atctcgttct cgttgagcga caaaatggca gagtcaatac
     4321 ccccgatcaa tccgagcgtg cccccgtacc ggtttcgtat acgcggatag gacaccccgg
     4381 cgaccccgct ttcacccgac cagaaaatcg aagcgccaag gtctctgaac gcaggtatca
     4441 gagaagcggt gttcccgtat gtctggaaca cgacgtgtga gacgccggcc ttccgcgcct
     4501 gatcaagcaa cactttgtac gccgccccgc atacttcgcg cagcatttcg ggggaaatga
     4561 cgggcgcgca attggaggcg atcggttcgg aaaacaccac gaaatcgaac ttcacagagt
     4621 cgtacgcacg ctggagcacc tgcatcgcga attcggctgc ttcgcgcaac cggtgggaca
     4681 tcgtttcagg ttcgtcatgc agggcgatca ggagtggcgc gagggaattc cagtccgcga
     4741 caccaaacgt caggaacaat ccccgcgata cacgcaaacc aaccgcgaaa tcacgggtag
     4801 aaaacgagac aacctgctgg tcccagtcgc gggggtacct ccggatgtcg tcggcgggac
     4861 aggtttgcac aggctcggga tctgttaccg gagtgaacga gaaatcgacg tgccgcaagt
     4921 cgggctgaac aacctcaaat cgttcgatcc ccagtgtgcg ttcccaaccg gcgtcaggac
     4981 ggaaaccttg cgatgcccag tcttcgacca cgtcatcccg gatgccgtcc ccccagagcg
     5041 ggggcgagca ccggctcgtt ccggcaaacg ttgcaacgaa acgctctctg ctggtcaggt
     5101 ccaccgctcg agcttttttc tgcggtctcg gagcttctcc ttcaccactt ttctgtgctt
     5161 gtcgctatct tcgtccatct tcaccgcact ctcgagaaga acttgaattg ccttcagatg
     5221 cttgacaatc ttccctcttc tcgttccgtc gattggcggt gttgagaccg ggagcgcgtc
     5281 cacaacagcc ccgagctttt cgagatcctt catgaacttt ggcttaagcc cgtatttctg
     5341 cggttgcgcg agcaggtcgc tgacaagcac cataatggaa ccctggtgtt ccgtcacggt
     5401 ctcctgccac tgcatctgcc cttccgtcag gtccgaggcc tgcgcgtccg tgctggcagt
     5461 tgcgtcagac ccgatttcct gcccggtcag aacctctttg atccgcacgg cgagcaatcg
     5521 tccgataccg cgaacgtgca tcagcttctc cagcggaacg tcccgcgccg cttgaaggtc
     5581 cttgataccc gcggctttca gccatttcgc ctttgtcggg ccaaccccgg ggaggtcgtc
     5641 gagagtcttc accaccgccc caccagcaga tgccttcgtt tcgtccacca tgtccccctc
     5701 cgcttgtcaa actttattcg tcgacagtcg gcgcaacaac atccgccccg gccgactggc
     5761 tgagcgttgc agaaatggca gcgcgcaatt cacgctggat ttcgtccact ttcttccgcg
     5821 cgtctataac ggtgaacccg tactcgtctg acatcttgtc gaattctcgg ataacgtccg
     5881 actggtattt gatgaaactc tcatacggat caagctgggg atactgatcg aggcctgctt
     5941 cccagtggtc gaggctgtct gccagcaaca cgcgatgaat gagcgtgttg acgtccacac
     6001 gcaggtatac cacgagatcc ggctcgatgg ctatgccgta caggcgccga atccattccg
     6061 ggtcagtgcc ccgggccagc gcgcgggcaa acgcggtgta cacgtaacgg tccgccagaa
     6121 cgacgaaacc actccgcagt gccgggatga tttcgtgctc caacctgtca gcaaaatccg
     6181 tcgcatacag caaattgaac gtgagcaggt tcagcgcgtt tcccgctttc gcaagatcta
     6241 tgctttccct gaccagtgcc gagcgagtcc aaccggtctc gatcactccg tacccttgaa
     6301 cctccagcca cgtgcgcagg agggagagtt gtgtcgtccg gcccacgccg tccgagcctt
     6361 caaccacgag caggtgcccg ttgacttctg aaaggtcaac gtatggcagt cccgttccca
     6421 tgaaccgtcg ttttgccatt gcgtccttcc tttttcaccc gtcgttctgg gtaccggctc
     6481 tgcgaaccgt cgagtccttt gattcgttcc cggttttctg ttccttcgcc gccagatacg
     6541 gagcgagata cgactgaagc tcccgcgaga caagcgccct gaactgcttc tgctgagctt
     6601 cgatcggttg aacggcatca atcacatgga aaccgaattc ttccgacatg cgatcatatt
     6661 cgtcgagaac acggctctgg aacagctcga agcactcgac cggatccttg ctcaaccccg
     6721 tgtccatgcc tgcttcatag aatttgagct tcatgcgcgc gctcagaagg cgtctgaggg
     6781 aaacgcggat gggcactttg aaatacaccg caagatcagg ctttatcgcg aaatcgtaca
     6841 gagatcggac ccattcggga tgaactcccc gcgcagtatc cctcgcaaag gccgtgaacg
     6901 cgtaccgatc ggcaatcaca accatccctg cctggagcgg gggtatgatc ttgtaagaaa
     6961 gccggtcggc aaaatcggta acgtgaagca ggctgaacgt agtaggggta agcgtattgt
     7021 ccttcttcgc cttctttgtg gagcttttca caagagctga cgaattccat tccgtccaga
     7081 acaccggcac gccaagggat accagccatt gccggagcag cgacgcttgc gtcgtctttc
     7141 ccgaaccatc gataccttcg acgatgatca agcgtccggg aaacgtgtgc ttttcatgga
     7201 gtcgtgccat tttcgccttc tctttcggga aaaccgccgg aggagaccac gatgaatttc
     7261 acaggcacac cgagaagctc ctggagaacg tcgctttttc tccctgcggc ccgtaattcc
     7321 tcatccactc ccccgaccgc cttgcacgaa atagtgcagt cgttctgcga tacccgcatc
     7381 tgggcggacg tgataagtcc gcgatgtgtt ctgtcaaacc cgtccgcgat tctgagcaca
     7441 ccggcaagac gccgaacgga ctcccgatcg tcgtcgccga gaccatcaaa taccggatcg
     7501 tccgcatccg ggaaacgctt tcggtgcgca cgcgccactg ccgcaatgat ctgcttttcg
     7561 ctccgtcgga atccgatcag atcggccctg cagatcatcg cctccgaaac tttgtggtgc
     7621 ctgcgaacgc cattaatata acctatatcg tgtaatagag acgcgtactc aagccattca
     7681 cgctcccatt cccggttttt ttttggcggc ggcagacctc cgtacaactc gagggcgagt
     7741 ttcgtcacct gcaccgcatg cgcttcctca aagtcccacg atctggccag ctcgattacg
     7801 gccgctttcc tggcgctgcg agcaatagat gccgattcca agcccttttc acccattcgc
     7861 aagggactca ataacccgct catcgagttt cttctggagc tccggccacg tgtttttgaa
     7921 agccttccat gctttcatcc gttccagttg gcacctcttc cggagccatt tcaccgcaac
     7981 gttcctctcc gattcccgcc agacgctgag gcgcacatcc gcgtcatgca catcccccaa
     8041 cgcgtcctgc acgttcacca gcgcgcggag cacgtcgctc cggtccttgc ccagaaccct
     8101 gtaccacgcg tccactgcat accgcatacg cctgcactcc cgccgcagcg cgtgctgtcg
     8161 ttccgacgac gactccttca cggtgcccac gtagcgcaat atcccccgag cacggcgcgc
     8221 cagcactttg cggatttcgt ctcccagaac ttcctccgca ccaggaagaa tgtgcactgc
     8281 gccggttccc cgggccaccc agtcgtccag acgaatcaaa agggcactga aacgagcgct
     8341 tctgagacca gccagaagcc ttctgagctt ttcccgtctt cttcgcgcgg cattttcccg
     8401 caacctgagc aacccttttt tcacgtcatc cgggcatgcc gtcatcatct tgtcaagcca
     8461 aagaattgtg atatccaggt cgcggacatt tccaagcaat ccgccgaacc acaccagttc
     8521 tcgtgaaagc tcgttgtacg cggtttcctc ccagatgaat cggtaaagcc gcaaaccggc
     8581 gcggattcgc cggagcgcaa ttcgcatgtc atgccgcggc ttcaccgaca ggattgaccc
     8641 tatgcccggt atcgcggccg ttacctgggt gaggtatccc cgcagtatga tacacgccga
     8701 ctcccccact gagtccttcc gccggatggg gggacgccag gggctgcggc ggcgggcagc
     8761 aacaatttgc ggtggcgccc cgatgcacga ttcccacaga tcggctttct tttccgcacg
     8821 cgcaacgtcg tgatcaagcg tgtcctgatt acctgcaaca ccaactactg gccttcttcg
     8881 gcccttcact gcgatcacac gggtggtctg ctccatgttg gaatccaacc cgtcggcgat
     8941 ccgcacgatc gcagccagtc tcagtgttgt ctcctgaaga tccgggggca attccatcca
     9001 gaggggctct gcctcgggaa caacctgctt tcgatgaaat gcaacgatgc aggcgaccat
     9061 atgtgtgtgg tcagtcggca gttcgtgtgg tttgagattc aggggagtga agccacgaag
     9121 cccttcgttc gcaacgatat cgcgcccgcg caggtgatgg ttttccgggt cctcgtagaa
     9181 gcccaca
//
LOCUS       DAOTNG010000107         9156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6915_length_9156_cov_5.335238, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000107 DAOTNG010000000
VERSION     DAOTNG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_9156_cov_5.335238"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="P5179_06665"
     CDS             <1..860
                     /locus_tag="P5179_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRS94935.1"
                     /translation="KSVTPIDPSLVQRVTVKRGGRDSMVIERRGNLWWMTSPLERSVD
                     RDTVNALLKEVQMSRVAVFHDTPAAMGTYGLDRPVAEVEFLMAYQGRLARRTLLLGSK
                     VPNVPNEQSGGQPVYYVKDADRPPVMEIPGGLGYNLKRPMEFYWDRHLAVFERRDINR
                     VELVTPERTVAMSADTLANWRFESPLSGGVKRWQVNGIVANLDMARVKQFVERPVAAT
                     GLDRPRLSVRLYHDNELKASVDFGAPHGGLVFAKGSNNNSPVMVDQQTMALFMLSVDD
                     LKELPIPPQ"
     gene            995..1981
                     /locus_tag="P5179_06670"
     CDS             995..1981
                     /locus_tag="P5179_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94936.1"
                     /translation="MSWVHRVSLAALVGIFVCAGTCPGFVEVAARVDTGAVLIGDVIT
                     YEILVTYDDTIVVEKLPHGVNLTAFHIRDYRAIPKTKLANDLWQEGGQYRISVYRPGL
                     YVIPPVPVRYHGRSGQSGELITQPITVEVKSIGVTEEDTLRGIKGPKSVPVRFPIWIW
                     GMPAVMVLAGVAVLFAVKRRGRKSTTAVEPPATIVLDEVAEFDRIPAADLIRSGDLKL
                     LYTLVSDAMRRYLARRYGVNSMELTLHELALSLESVGADGNDVGDMISFLERCDLVKF
                     ARFIPAQDEVETLIERAKNLVRQTQVSTVAAVSETSPKNAADDRDGEGSTSV"
     gene            1978..2988
                     /locus_tag="P5179_06675"
     CDS             1978..2988
                     /locus_tag="P5179_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS94937.1"
                     /translation="MTFSFASPWWLLLLMVLPLQVWWHMRGTHFTNAVLRLPLATSLR
                     QFRPSTRVRLRHVVLVLQILGEFTLIVALARPRSGKVEQRVISEGVDIVLALDVSGSM
                     QAFDMGNRERIDVAKEVTSQFIDSRASDRLGMVVFAGQSYTQCPLTVDYGILKNLLAQ
                     VSIRMNETIPDGTAIGMALATATNRLRKSTAPSKVIVLLTDGSNNAGIIDPLTAAEAA
                     ARLGIRVYAIAVGVTGRAPIRVRDPLFGERIEFMEDSINEDVLREIAAITGGRFFRAT
                     SQTALQEIYREIGMMEKTEVATLQIPRFAELTFVFHLIPLGLGLLILGSFLRFTVFRT
                     IP"
     gene            2993..4045
                     /locus_tag="P5179_06680"
     CDS             2993..4045
                     /locus_tag="P5179_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS94938.1"
                     /translation="MEYGTVDILISLVTQLWRDPEWLWGLLLVPIVPLAYFVLEWRAR
                     SLITRLGNLDVLTRSAIEVSTRLRMVRLCLFSGAIAFGFAAAARPQWGSSTEIVHSTG
                     LDIVLLLDTSLSMLAEDLKPNRLESAKLEVATFLDKLKSDRVALVPFAGTAFIQCPLT
                     LDYSALRLFLSDITPYTVNEEGTQIGTAIRTGLKAFGEGDDRSRVMVLITDGEDQGSN
                     PVEAAQMAADEGVRIYAIGIGSPEGELIPDTGIDGRPDFLRDDQGSVVKTKLDEEALR
                     EIAIETGGEYYRASPGGRELEAILESISDMERTRQGEFQTVARIDRYQTPLACSVLLF
                     LVIPFVPERAGTRRRS"
     gene            4042..4710
                     /locus_tag="P5179_06685"
     CDS             4042..4710
                     /locus_tag="P5179_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94939.1"
                     /translation="MTKVSAYVLVLAVWLFGCELSDVLVQRSVARKVQEGNALYLRGE
                     LNEALIRYRDAETDAPEEGRIKFNIGNVFYRQAQFGEAEETYLDAARRGDAVSRADAW
                     YNLGNACFKQGRFDKAVESYQKALEFNPSDRDAKFNLELVQKMLNRAASEAASTPAGY
                     RRPSNWARDRMKEAEALAQRGKYGEAQRVISRTFQAEPSCVGEFGEFAERVNALKAIF
                     GEGR"
     gene            4707..5234
                     /locus_tag="P5179_06690"
     CDS             4707..5234
                     /locus_tag="P5179_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94940.1"
                     /translation="MNGVLFFAVALALGGGTYATGQQTVSGLPPELLVARAAQAVATI
                     AFDRSLEEQALRDLAAALRQDPRNRHAKALVDLIQKRRQEREKQEQQRGQQQLRNQRP
                     EPQPQVAQQQPGTKQKVSSNAPDRTETNRRAPREEGNRLTREQAQQLLDALEQQDRRP
                     TQRLPVRNPRQGPRW"
     gene            5264..7045
                     /locus_tag="P5179_06695"
     CDS             5264..7045
                     /locus_tag="P5179_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRS94941.1"
                     /translation="MCNKILTGVTTLLLLASMGRAEVQVFARVDRNQAQVGEQIILTV
                     AVVSQKPLNVQVPPTLRISGTSCSQVRGPVTSEEMSISGGRLLRRSTAEYAYFLTTER
                     AGEIEIPAIPVAVEGTQFLTQPIRLTVGKSPVLEAGGPVQVRVNVSRTTVYQGEQIIV
                     EYLLYEEAVASSRIQNRQLVRDPSFLNFWAETLEDIRRQQIPTRRETINGVAYNVLPI
                     LRYVVFPTRAGTLTIPSITVSLVAALPTGARTIWGEPVLQPRQISVNSPERTIESRAL
                     PANPPAGFSGAVGSFSIVSEVDKNEVAEGDPITWKVIIEGDGNVKSVGDLRIPDLTDF
                     RAYDPKTTASFTLAQGAVRGKLVYERVVMPLHAGISQIPGTTFTFFDPRTRQYRTIAV
                     EAKKITVTANRAAVARTPGGGQMLVKEVGSDIRYIQPDVNSLDDESWRLTDSPFYWVS
                     YGLPVAWVLLAFGVRARRIRMGANPARMRALGAAGEARRRLKAARLALEKNDVHGGIQ
                     AVANALTELVAAAANTTAAGLTSSRISEVLSEKEVPENLRKEVLATLAQCDEIRYAPT
                     SASVDDAKAIYEKGLDLVRQLLRYLVP"
     gene            7042..7812
                     /locus_tag="P5179_06700"
     CDS             7042..7812
                     /locus_tag="P5179_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS94942.1"
                     /translation="MKRTVATVFLLVLILAVARSSGASNPGPARLFNEGNAAYRQGRY
                     EDAIAAYNKVLAGGVRSSAVYYNLGNSYYRTGKLGFAILNYERSLRLDPANDDTRHNL
                     RFVSSLVQDRVLVSSFELKFQEKLSQFLGYTNPNLVAVIVLLAVFVSGGIGGLWLLGR
                     FRTRIAMVIFVGCCVLAALGASVMAGQKWAADEGRTGIVLADQTNARFEPSETSRVVF
                     VVHEGTKVWLIRRDRGWAFVRLANGLRGWVQQGAFEMI"
     gene            7824..8711
                     /locus_tag="P5179_06705"
     CDS             7824..8711
                     /locus_tag="P5179_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRS94943.1"
                     /translation="MASRLFAWGAALVVLAGQVWAQPSYLVPLLNEIERTNPASFYTV
                     TFRLYERVGAGVYSPEDLRFLSSALLRRAEASPELLPVVLPLLGQMNVPETVPPIMTH
                     ARSSDQAVRTAAYQALGWMGDERALPLLTARNRQERPAASAEERFMIEYATRSIELKA
                     RLRRMSSGDQFGLVRTTLLTEPNWLVRGDIARMLSKRAGADVWAVLFDSYERWATTPQ
                     YVQMIGEVLGQRYRFDPTGYIAAVRRRPPETRLYALERISDFPHISDMGAIMDISETD
                     SDPIVRERATILLGKLLNR"
     gene            complement(8708..>9156)
                     /locus_tag="P5179_06710"
     CDS             complement(8708..>9156)
                     /locus_tag="P5179_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS94944.1"
                     /translation="GYVHTECTDVYDSHCYEQDVAKFAANFAGMISGQEVWRNFPKED
                     AEWAGQPYFVSEYGGIWWNPGKEDGKSWGYGDRPKTEEEFLTRYRGLTEALLNHPMMC
                     GFCYTQLYDVEQEVNGLYTYQREPKFDPAIFREINRQKAAVERTES"
BASE COUNT         1922 a         2450 c         2829 g         1955 t
ORIGIN      
        1 acaagtcggt gacgcctatt gacccatctc tcgtgcagcg agtaaccgtg aagaggggtg
       61 gcagggactc gatggtgatc gaacgccgcg gcaatctctg gtggatgacc tctccgttgg
      121 aacggtcagt ggatcgggac accgtgaacg cgctgttgaa agaagtccag atgtcccgtg
      181 tggcggtctt ccacgatacg cctgctgcga tgggaacgta cggtctggat cgacctgtgg
      241 cagaggtcga gttcctcatg gcataccagg gacgtctggc cagacggaca ctgcttctcg
      301 gttcgaaggt tccaaatgtt ccgaacgagc aatccggggg gcaaccggtg tactacgtca
      361 aggatgcgga ccggccgccc gtgatggaga ttccgggagg gcttgggtac aacctgaaac
      421 ggccgatgga gttctactgg gatcggcacc tcgcggtttt cgagagacgt gatatcaacc
      481 gggtcgaact cgtgactcct gagcggacgg tggccatgtc tgctgataca cttgccaact
      541 ggcggttcga gagcccgctg tccggcggag tgaagcggtg gcaggtgaat ggcatcgtgg
      601 caaatctcga catggcacgt gtgaaacagt tcgtggaaag gccggttgcc gccacgggtc
      661 tggataggcc ccgcctcagt gtccgcctgt accacgacaa cgagttaaaa gcctcagtgg
      721 atttcggagc accgcacggt ggacttgttt ttgccaaggg gagcaacaac aacagcccgg
      781 ttatggtcga tcaacagacg atggctcttt tcatgctgag tgttgacgat ctgaaggaac
      841 tgccgattcc gcctcaatga tgttctggca agcggggact ggcatcagaa ggacggtgcc
      901 acggcagaca gcagcagtat gcaacagggg gtgcgatcta tcgcgccctc ttttgctgaa
      961 aaaggtttgc acttactctg gcaggatcat gccgatgtca tgggttcacc gggtgagtct
     1021 ggctgcactt gttgggatat ttgtctgcgc cggtacctgc ccgggtttcg tcgaagttgc
     1081 tgctcgtgtg gataccggtg ccgtcctgat cggggatgtc atcacgtacg agattctggt
     1141 aacgtacgac gatactatcg tggtagagaa gcttccccac ggggtcaacc ttaccgcctt
     1201 ccacatcagg gattaccggg ccattccaaa aacgaagctt gccaacgacc tgtggcaaga
     1261 gggtgggcag taccgcattt cggtgtaccg tcccggcctt tacgtaattc cgcccgttcc
     1321 cgtgcgatac catggcaggt ccgggcaatc gggggagctg attacgcaac cgatcacggt
     1381 tgaagtgaaa agcattggcg tgaccgagga ggatacgtta cgtggcatca aagggccgaa
     1441 gtccgttccg gttcggttcc cgatctggat ctggggtatg cccgcggtga tggttctggc
     1501 gggagtcgcg gtgctttttg cggtgaagcg tcgaggacga aagagcacca cggcggtaga
     1561 accccccgca accatcgttc ttgatgaggt cgcggaattc gatcggattc ccgctgcgga
     1621 tctgattcgg tcaggagatc tcaagctcct ctacacgctc gtgtccgatg caatgcggcg
     1681 gtacctcgcg agaagatacg gcgtcaactc gatggagctc acgctccacg aactcgctct
     1741 gtctctggaa tccgtcggtg cggacgggaa tgatgtcggg gacatgattt cgttcctgga
     1801 gcggtgtgac ctcgtgaaat tcgcgcgatt tattcccgca caggacgagg ttgaaacgct
     1861 tatcgagcgc gcgaagaacc tggttcggca gacgcaggtc tcaaccgtcg cggcagtatc
     1921 ggaaacgagt ccgaaaaacg ccgctgacga tagagacggt gaggggagta cgtctgtatg
     1981 accttcagct tcgccagtcc gtggtggctt ctccttctca tggtcctgcc gttacaggtg
     2041 tggtggcaca tgcgtggcac gcacttcacg aacgcggtgc tgcggcttcc tttggcgacg
     2101 tcactcaggc agttcagacc gtcaactcga gtcagattgc ggcacgttgt gctggtactg
     2161 cagattttgg gagaattcac actgattgtg gcccttgcgc gtccccgatc cggaaaagtc
     2221 gaacagcgtg tcatttctga gggcgttgac atcgttctcg cgctggatgt cagtgggagc
     2281 atgcaagcct ttgacatggg taaccgggag cggatagacg ttgccaagga agtgacttct
     2341 cagttcatcg attcacgcgc ctccgaccga cttggaatgg tggtatttgc ggggcagagt
     2401 tacacccagt gcccattgac ggtggattac ggtatcctga agaacctgct ggcgcaggtg
     2461 agcatccgca tgaatgagac cattcccgat ggtacggcga tcgggatggc attggctacg
     2521 gcaacgaatc gactcagaaa gagcactgct ccgagcaagg tgatcgtcct gctcaccgat
     2581 gggagcaaca acgcggggat catagaccct ctgaccgctg cggaagcggc tgcacgactg
     2641 ggaatcagag tgtacgccat cgcggttggc gtcaccgggc gcgcgccgat tcgcgttcgc
     2701 gatcctcttt tcggggaacg tatcgaattc atggaggaca gcatcaacga ggacgttctg
     2761 cgggagattg ccgctatcac gggcgggcgc tttttccgtg cgacgtctca gacggctttg
     2821 caggagatct accgggaaat cggcatgatg gagaagacgg aggtggcaac gctccagatt
     2881 cctcgttttg cggagttgac ctttgtcttt catctgatac ccttggggtt gggattgctg
     2941 atcctcggct ccttcctgcg cttcactgtg ttcaggacga tcccatgaag tggtggagta
     3001 cggaactgtg gacatcctga taagccttgt tactcaactc tggcgggatc cggaatggct
     3061 ttgggggctg cttctcgttc ccatagttcc ccttgcgtat ttcgtgctgg aatggcgtgc
     3121 gcgttcactg attacccgtc tcggtaacct cgacgttctc acacgatcgg caatcgaggt
     3181 cagcacccgt ctgaggatgg ttcgcctgtg tctgttcagc ggggcaattg ctttcggctt
     3241 tgccgcagcg gccaggccac agtggggatc atcaaccgaa atcgttcaca gcaccgggct
     3301 ggatattgtg cttctgctcg acacatcgct ttcaatgctc gccgaggatt tgaaacccaa
     3361 ccggctggaa tcggcgaaac tcgaagtggc gacgtttctg gacaagctca aatcggaccg
     3421 cgtggccctc gttccgtttg cgggtacggc tttcatccag tgtccgctga cgttggacta
     3481 ctccgctttg cgactttttc tcagtgacat taccccgtac acggtgaacg aagaggggac
     3541 acagattgga accgcgattc gcaccgggtt gaaagcattc ggcgaaggtg atgacagaag
     3601 tcgcgtgatg gtgctgatca cggacggcga agatcaggga agcaatcctg tggaagcggc
     3661 ccagatggct gctgatgagg gcgtgcggat ttacgcgatc ggtatcggat ctccggaagg
     3721 tgaactcatt ccggatacag gcatagacgg ccgtccggat ttcctgcgtg atgatcaggg
     3781 aagcgtggtg aaaacgaaat tggacgaaga agcactgcgg gaaatcgcga tcgagacggg
     3841 cggcgagtat taccgcgcga gcccgggtgg gagggaactg gaagccatac tggaaagcat
     3901 aagtgatatg gaaagaaccc ggcagggtga attccagacg gttgcccgta tcgaccggta
     3961 ccagactccg ctggcatgct ctgttctgtt gttccttgtc attccgtttg ttccagaacg
     4021 ggcgggaaca cggaggcgat catgacaaag gtgagcgcgt acgttcttgt gctggcggtc
     4081 tggctgttcg gttgtgaatt gagtgacgtg ctggtacagc gttcagtcgc ccgcaaggtg
     4141 caggaaggga acgcacttta tctccgggga gaactgaacg aagcgctgat cagataccgc
     4201 gatgcggaaa ccgatgcgcc ggaagaaggg cgtatcaagt tcaatatcgg aaatgtgttc
     4261 tatcgtcaag cccagttcgg tgaagcggaa gagacatatt tggatgccgc gagacggggc
     4321 gacgcagtgt cgcgggcgga tgcctggtac aacctcggca acgcgtgctt caaacagggg
     4381 cgttttgaca aggcggtgga aagttatcag aaggctctgg agttcaaccc gtctgacaga
     4441 gacgcgaagt tcaatctcga actggttcag aaaatgctga accgtgcggc aagcgaagcg
     4501 gcgagtacgc cggccggcta ccgacgcccg agcaactggg cgagggatcg gatgaaagaa
     4561 gcggaggcgc ttgcacagcg aggtaaatat ggcgaggctc agcgagtgat ttcacggacg
     4621 ttccaggcgg aaccctcatg tgtcggcgag ttcggggaat tcgccgaacg ggtgaatgcg
     4681 ctgaaggcaa tattcgggga aggccgatga acggcgtttt gttctttgca gttgctctcg
     4741 ccctcggggg cggcacttac gcaacaggcc agcagacggt ttctggtctg cctcccgagc
     4801 ttctggtagc aagagccgcg caagcggttg ccaccattgc gtttgatcgc tcccttgagg
     4861 aacaggcgct tcgcgacctt gcagcggcgc ttcggcagga ccctcgcaac cgccacgcaa
     4921 aagctcttgt cgatctcata cagaagcgtc gacaggaaag ggagaaacag gagcagcaga
     4981 gagggcaaca gcagttgcgc aaccaacggc ccgaacccca gccacaggtc gcgcagcaac
     5041 agcccgggac gaaacagaaa gtgagcagca atgcgcccga ccgcacggag actaatcgtc
     5101 gggcaccgcg cgaagagggc aacaggttga ccagagaaca ggcgcaacag ctcctcgacg
     5161 cgcttgagca gcaggataga cgaccaacgc agagactccc ggttcgtaac ccccggcagg
     5221 gaccacgatg gtgaaagtcg tgggttgtgt ggtgcgaatg cgtatgtgca ataagatcct
     5281 caccggtgtg acgacacttt tgctcctggc gtcgatgggc cgcgcggagg tacaggtgtt
     5341 tgcgcgggta gaccggaacc aggcacaggt aggcgagcag atcattctca ctgtcgcggt
     5401 cgtgagtcag aaaccgctca acgtacaggt acccccaaca ctgcggatat caggtaccag
     5461 ctgttctcag gtgcgcggcc cggtaacttc agaggaaatg tctatttctg gagggcgtct
     5521 tctgagacgt tcgaccgccg aatacgccta ttttttaacc acggagcgcg caggtgaaat
     5581 cgagattccg gcgattccag tggcagttga gggcactcag tttctgaccc agccgatacg
     5641 tctgaccgtg gggaaatcac cggtactgga agccggagga ccagttcagg tgcgggtgaa
     5701 cgtgagccgc acaacggtgt accaaggtga gcaaatcatc gtggaatatc tgttgtacga
     5761 agaagcagtg gcgagcagca gaatccagaa ccgccaactt gtgcgcgacc cttcattcct
     5821 gaacttctgg gcggaaacgc tggaggatat ccgccgtcaa cagattccca cccggaggga
     5881 aacgatcaac ggggttgcgt acaatgtgtt gccgatcctg cgttacgttg tgtttcccac
     5941 gcgggcgggc acactgacga tcccatcaat caccgtgtcg cttgtggcag ccctgccgac
     6001 cggagccagg accatctggg gcgaaccggt tctgcaaccg agacagatca gcgtgaactc
     6061 gcctgagcgc acgattgagt cacgagcgct tcccgcaaac cctcccgccg gcttcagcgg
     6121 ggccgtcgga tccttttcga ttgtgagcga ggtggacaag aacgaagtcg ctgagggaga
     6181 ccccataacc tggaaggtga tcatagaagg cgacggaaac gtgaaaagcg tgggcgatct
     6241 gcgcattcca gatctgactg atttccgcgc atatgatcct aaaaccactg cttctttcac
     6301 cctcgcacag ggcgcggtgc gtggcaagtt ggtttatgaa cgcgtcgtga tgccacttca
     6361 tgcgggtatc agccagattc caggcactac ctttacgttt ttcgatccca gaacgaggca
     6421 gtatagaacc atcgcggtgg aagcaaagaa gatcactgtt acggcgaaca gggcagcggt
     6481 tgcgcgtacg cctgggggcg gacagatgct ggtgaaggaa gtcggctcgg atatccgcta
     6541 catccaaccg gatgtcaatt cgttggacga tgagtcatgg cgtcttaccg attccccgtt
     6601 ctactgggtg tcgtacggcc ttcctgtggc gtgggttctt cttgcatttg gcgtacgggc
     6661 gcgcagaatt cgtatgggcg cgaacccggc acggatgcgt gcgctcggcg ccgcgggaga
     6721 ggctcgtcga cgtctgaaag ctgcacgctt ggcgctcgag aaaaacgacg ttcacggtgg
     6781 gattcaagcc gttgcgaacg cgttgaccga actggttgct gcggcggcca acaccactgc
     6841 ggcgggcctc acttcgtcgc gcatatccga ggtcctcagc gaaaaagaag tgccggaaaa
     6901 cctcaggaaa gaagtcttgg cgacgcttgc gcagtgtgat gaaatccgct acgccccgac
     6961 ttccgcgtcg gtcgacgacg cgaaagcgat ctacgagaag gggctcgacc ttgtccgcca
     7021 gttgctgaga tacctcgtgc catgaaaagg actgtcgcca ccgttttcct actggttctg
     7081 atcctggccg tggcgcgctc atcgggcgct tcaaatccgg ggccggcccg tctgttcaac
     7141 gaggggaacg ctgcgtaccg gcaggggagg tatgaggacg cgatcgccgc gtacaacaag
     7201 gtgctggccg gaggagtgcg ttcaagcgca gtttattaca accttgggaa ttcttattac
     7261 cggacgggca aactggggtt tgccattttg aattatgaaa gaagtctccg cctcgacccc
     7321 gccaatgatg atacccgtca caacctcaga tttgtgtcct cgctcgttca ggaccgtgtt
     7381 ctggtttcca gcttcgaatt gaaattccag gagaagttgt cgcagttcct cgggtacaca
     7441 aaccccaatt tggtggcggt tatcgtactt ctggcggtgt tcgtctctgg cgggattggc
     7501 gggctttggc ttcttggacg gttccgcacg cgcatcgcaa tggttatttt cgttgggtgc
     7561 tgcgtgttgg ctgctttggg cgcaagcgtc atggccggcc aaaaatgggc ggccgacgaa
     7621 gggcgcaccg ggatagtgct tgccgaccag acaaacgcgc gttttgaacc gagcgaaact
     7681 tcgcgggtgg tgttcgtcgt tcacgaaggt accaaagtct ggctcatacg acgcgaccgt
     7741 gggtgggcat tcgttcgact tgccaacggt ctgcgcgggt gggttcaaca gggtgcgttc
     7801 gaaatgatat gaaggagtgg tgtatggctt cgcgcctgtt cgcatggggc gcagcgttgg
     7861 tggttcttgc cggccaggtg tgggctcagc cttcgtacct ggttccgctc cttaacgaaa
     7921 ttgaacgaac caacccggcc agcttctaca ctgtcacctt caggctctat gagcgagtcg
     7981 gggcgggtgt ttattcgcct gaagacctgc gtttcctcag cagcgcctta ctccgccgcg
     8041 cggaggcctc acctgaactg ctgcctgtag tcctcccgct tctgggacag atgaatgtcc
     8101 cggaaactgt gccgccaatc atgacccacg cgcgatcatc tgatcaggcg gtaaggacgg
     8161 ccgcctatca ggccctggga tggatgggcg acgaaagggc tctccctctc ttgacggccc
     8221 gtaatcggca ggaaaggcct gcggccagcg cggaagagcg gttcatgatc gaatacgcga
     8281 cacgatcgat cgagcttaaa gcccgattgc ggcgcatgtc gtccggagat caattcgggt
     8341 tggtccgcac taccctgctc acggaaccga actggctggt gcggggtgac atagcgagga
     8401 tgctgtcaaa aagagcaggt gcggatgtct gggcggtctt gttcgactcg tatgaacgtt
     8461 gggcaactac tccgcaatac gtgcaaatga ttggcgaagt gcttggccag cggtacaggt
     8521 tcgacccgac gggttacatc gctgcggtga gacgacgtcc tccggagacc cgcctctacg
     8581 cgttggaacg catttccgac ttccctcaca tcagcgacat gggcgccata atggacatct
     8641 cggaaaccga ttcggatccg atagtgcgcg agcgcgccac aatcctgctt ggaaaactgc
     8701 tgaaccgtta ggattcagtt ctttctaccg cagccttctg ccgatttatc tccctgaata
     8761 tcgcgggatc gaacttcggt tcacgctggt aggtgtagag cccgttgacc tcctgctcca
     8821 catcgtacag ctgggtgtaa caaaaaccac acatcatcgg gtgattcagc agagcttccg
     8881 taagacccct gtaacgtgtg aggaactctt cttccgtttt tggccggtca ccgtatcccc
     8941 acgacttgcc gtcctctttt ccggggttcc accagatccc gccgtactcg ctcacgaagt
     9001 acggctggcc cgcccattcc gcatcctctt tggggaagtt gcgccatact tcctgccctg
     9061 aaatcattcc cgcgaaattg gccgcaaact ttgcaacgtc ctgttcataa caatgtgaat
     9121 cgtatacatc tgtgcattcc gtgtgaacgt aaccgg
//
LOCUS       DAOTNG010000108         9153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6917_length_9153_cov_5.098263, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000108 DAOTNG010000000
VERSION     DAOTNG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6917_length_9153_cov_5.098263"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5179_06715"
     CDS             <1..392
                     /locus_tag="P5179_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94945.1"
                     /translation="NDMNTVHGGFCTIEDCAARMICTVVSTAIPGQFVIDGGTKTFTS
                     DRCIPRPESGHGYIIEYPEARIFALSEEHGQVDARACSRVPKVGERVTVIPNHICPCV
                     NLQDSFWIAEPGNPLRRITVDARGKLS"
     gene            370..1331
                     /locus_tag="P5179_06720"
                     /pseudo
     CDS             370..1331
                     /locus_tag="P5179_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
     gene            1328..2179
                     /locus_tag="P5179_06725"
     CDS             1328..2179
                     /locus_tag="P5179_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS94946.1"
                     /translation="MIVDVHSHAWLYPEHFGAGFREQAQRARGQKEVDLSADFERYSD
                     AAPADTVAIVFGGKARLSGLWVDDRYVAEYVARHSPRVIGFLSVDPSQPGWETEMREG
                     HEVLGLRGIKLLPMYAGFRPDDRALDGLWSYAEKNRLPVILHSGTTFISQAPLECTLP
                     RWVDNVAIRFPDVRIVLGHLGHPFENEAVAVIRKHPNVYADVSALFYRPYQLYNSLML
                     VQEYGVWNKVLFGSDYPFSTVNESVSGLRSLNEMLTGTALPRLNRAEIEAMIHRDSLS
                     LLQLNRP"
     gene            2176..3372
                     /locus_tag="P5179_06730"
     CDS             2176..3372
                     /locus_tag="P5179_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS94947.1"
                     /translation="MNRFALTGARVWTGDGEAFDGYVIVSKGIIERVAQGACAEALPR
                     VDLSGYSLSPGMIDLMVMGGFDRSILRDDPLDIAKEYLRLGVTSCQFCIGTLPWDSMV
                     KVATNIRRALSCREYDSARVLGVYCEGPFQQPHLTGASLRDFALEPSPETVERLLADM
                     GNAVTMVNVAPGLPGDAEAVTRLVASGKIVSMAHSDAPAERVFRCVEAGTTVLGHAWD
                     NNSGRIGDSGVQQPTIEHVAMTDERIRYLHVIADGVHVHPVMMRLLLRARGSGALCLV
                     TDAVTRAGMPDGPYVWDDGRRFFKSGGVGRTDTGWLCGSATLLPDMFRNYVNFTGLPP
                     HEAIRAVTGNPAASLGMSDEIGHIAAGHIADLVAWDSHLRVRRVWRQGVEISNVSSYG
                     EVEISP"
     gene            3369..4193
                     /locus_tag="P5179_06735"
     CDS             3369..4193
                     /locus_tag="P5179_06735"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRS94948.1"
                     /translation="MTDRFSLDGKIAVVTGGSRGIGKGIAVGFAEHGADVAIVYRSAQ
                     KEAEAVAERIRGLGRRCWVFRRDLGEVETLSDFVDDIWRTTGRIDVLVNNAGMAYLER
                     FNEITAAHWRRLMAVNLDAVFFMAQRTAEHMIRDGIKGRIINLSSKNGLVAEAGLAHY
                     NASKGGVELVTKSLAIELGEHGITVNCIAPGIIETEIAGEFDIDPAFFSYYKEHIPLE
                     HRFGAVEECVGAAVFLASSAGSYITGQSLVIDGGVLCEQVPRMQFMSTFKNTLNGG"
     gene            complement(4370..5830)
                     /gene="guaB"
                     /locus_tag="P5179_06740"
     CDS             complement(4370..5830)
                     /gene="guaB"
                     /locus_tag="P5179_06740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRS94949.1"
                     /translation="MRDAIPEGLTFDDVLLVPGESDVAPADVVTTAPFTRQVPVNIPL
                     VSAAMDTVTEARLAIALAREGGIGVIHRNAPVEYQASEVEKVKRSESGMIVDPVTLGP
                     DASIREALTLMSELHISGVPVVDPHGKLVGILTNRDLRFERKLDQPIRNVMTSENLVT
                     APVGVSMEEARATLHRHRIEKLLVVDSEGYLRGLITVKDIEKKIQYPNACTDSLGRLR
                     VAAAIGTGADWEARATALVEKEVDVVVIDTARGHSRRVCSTVQAFKKRFPNVPVVAGN
                     VATREGTKALIDAGVDGVKVGVGPGSICTTRVVSGVGVPQITAVQECAECAQQYGIPI
                     IADGGVKHSGDITKGLAAGASTIMIGSLFAGTDESPGETIIYQGRTFKVYRGMGSLGA
                     MVQGSSERYLQNDVEPEKLVPEGIEGRVPYKGSLSAYVYQLVGGLRSGMGYVGAMTIS
                     ELHEKARFIRISQAGLRESHAHDVTITKEAPNYRVE"
     gene            6200..7021
                     /locus_tag="P5179_06745"
     CDS             6200..7021
                     /locus_tag="P5179_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRS94950.1"
                     /translation="MHTIMDKRLLAPNVYRLEVGAQLIAERRKPGQFIILRVNEESER
                     IPLTIVESDPVAGTIVLVFQVMGEGTRLLASLEMGDEIRDVVGPLGHPTEISHYGTVV
                     CVAGGIGVAPMFPIAKGMADMANTVISIVGARTRDLLILQEEIQSVSSEVLITTDDGT
                     FGRHGFVTDALKERLESGFTPDAVVAIGPLPMMRAVAETTRPYGIKTIVSLNPIMVDG
                     TGMCGGCRVSVGGQMKFACVDGPEFDGHQVNYAELANRQRMYRDKEACRIRSLIP"
     gene            7153..8514
                     /gene="gltA"
                     /locus_tag="P5179_06750"
                     /pseudo
     CDS             7153..8514
                     /gene="gltA"
                     /locus_tag="P5179_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
BASE COUNT         1924 a         2519 c         2792 g         1918 t
ORIGIN      
        1 tcaacgatat gaatacggtt cacggtggat tctgtacgat cgaggactgc gctgcgcgaa
       61 tgatctgcac agtggtcagc acggcgattc ctgggcaatt tgttattgac ggcggaacaa
      121 aaacgttcac gagtgaccgg tgcattccga gacccgaaag cgggcacggg tacataatcg
      181 agtatccgga agcacgcatc tttgccctga gcgaggagca cgggcaggta gatgcacgcg
      241 cgtgttctcg cgtgccaaag gttggtgagc gcgtgaccgt cattccgaat cacatctgcc
      301 cctgcgtgaa tttgcaggat tctttctgga tcgcggaacc cgggaaccca ctccgcagaa
      361 ttaccgttga tgccagagga aagctatcct gaaccttttt gtcgacgaaa ggcaaccgtg
      421 aacacctcca gcaatctgcg cggcgtgctt cccgtattcc agacgccgtt tcgagaagat
      481 tactcgattg atttcgacgt attgcgccat gagatagagt ggctttacga atgcggtgcc
      541 gacggcaccg tcatggcgat ggtctccgaa acactccgcc tttccacaga agagcggcgt
      601 gagcttgcgg cggctgcctg cagattcggg tgccagcgcg ggccggtggt tatcagcgtt
      661 ggggctgaat ccacatttgt tgcagagagt cttgcccgac acgcggagag cgtgggagcc
      721 actgcggtta tggcgattcc tcccgtatct gtagcagtgg gtgaggatga gctgttacgg
      781 tattacgaag ccatactccg cgggatacgg attcctctga tcgttcagga cgccagcggt
      841 tacgtgggca agcccatgtc aatcgagatt caggctcgcc tcctcaacga attcggagac
      901 gatcggattc ttttcaaacc ggaagccacg ccgatagggc agcgtctgac cgctttgcac
      961 cacgcaacgg gtggacgtgc acgtgtgttc gaaggcagtg gaggaatcgc cctcaccgag
     1021 agtttccgcc ggggtatcac gggcactatg ccgggcgctg atctcatcaa aggaattgtg
     1081 gcgttatggc acgccctcga ggcgacgagg agcgagtgta tgaattgagc tttcccattt
     1141 ctgccctggt ggcgatgcaa cacgggttgg acgggttcat tgccgtggaa aaataccttt
     1201 tgcacaaaca gggagttttc cccaacacgg tggttcgcgg gccggtgggc ttcgtgctcg
     1261 acgatgagac gaagaatgaa attgaccggc ttttctcaat actcgaaaag tcgctgcaga
     1321 gctcacgatg atagttgacg tccacagtca tgcctggctc tacccggagc atttcggagc
     1381 aggtttccgg gaacaggctc aaagagcgcg cggccagaaa gaagtcgatc tttccgcgga
     1441 tttcgagcgc tacagcgatg cggcacccgc ggataccgtg gcgatagttt tcggtgggaa
     1501 agccagactc tccgggctct gggtggacga ccgctacgtg gcggagtacg tcgcccgcca
     1561 ttccccacgg gtgatcgggt tcctttccgt tgacccgagc cagccgggtt gggaaacgga
     1621 gatgagagag ggtcacgaag tgctcggcct gcggggcatc aaactcctgc ccatgtatgc
     1681 gggatttcgt ccggacgacc gggcgctgga tgggctgtgg agttatgccg agaagaaccg
     1741 acttcccgtg attctgcaca gtggaacgac cttcatctcg caggcgcccc tcgagtgtac
     1801 attgccacgg tgggtggaca acgtggccat ccgtttcccg gacgtcagga tcgttctggg
     1861 tcatctggga catccctttg agaacgaagc cgttgcagtc atccgtaagc accccaatgt
     1921 gtacgcggat gtcagtgcgc tgttttatcg cccttaccag ctctacaaca gccttatgct
     1981 cgtccaggag tacggcgtct ggaacaaagt gctcttcgga agcgactacc cattctccac
     2041 ggtgaacgag agtgtttccg ggttgcgctc tttgaatgaa atgctcaccg gcacggcgct
     2101 gccccggttg aacagggcag aaatcgaggc gatgatccac cgtgacagcc tttctctcct
     2161 ccaactgaac cgcccatgaa tagattcgca ctgactggcg cccgcgtctg gaccggtgat
     2221 ggtgaagcct tcgacgggta cgtaattgtt tcgaagggaa tcattgaacg cgttgcgcag
     2281 ggggcgtgcg ctgaagcgct tcccagagtc gatttgtctg ggtactcgct ttcgccggga
     2341 atgatcgacc tcatggtgat gggcggtttc gaccggagca tacttcggga cgatcctctt
     2401 gacattgcga aggaatacct gcgcctcggg gtaacgtcct gccagttttg catcggcacg
     2461 ctcccgtggg attcgatggt aaaagtcgcg acgaacattc ggcgagcatt gtcctgccgc
     2521 gaatacgatt ctgcgcgagt actcggagtg tattgcgagg gacctttcca acaaccccac
     2581 cttacgggcg caagtttgcg tgattttgcc ctcgaaccct ctccggagac tgtcgagcgt
     2641 ttgctggctg atatgggaaa tgccgtcaca atggtgaatg tcgcccccgg gctgccgggc
     2701 gatgctgagg ccgtgacgcg tctggttgca tcagggaaaa tcgtttcaat ggcgcattcg
     2761 gatgccccgg ccgagcgggt ttttcggtgc gttgaagccg gcacgaccgt gctcggccat
     2821 gcatgggaca acaactccgg ccgcatcggc gattcgggcg tgcaacagcc aaccatcgag
     2881 cacgttgcga tgacggatga gcgtatccgg tacctgcacg tcatcgcgga cggagtccac
     2941 gtgcacccgg ttatgatgcg ccttcttcta cgcgcccgtg ggtcgggtgc gctgtgtctg
     3001 gttaccgatg ccgttacccg ggccggcatg cctgatggcc cctatgtctg ggatgacgga
     3061 cgccgtttct tcaagtccgg cggcgtgggc cgaactgaca ccgggtggct ttgcggcagc
     3121 gccactctgt tgcccgacat gttccggaac tacgtcaact tcaccgggct tccgccacac
     3181 gaggcgatcc gggcggtgac gggcaatccg gcggcatcgc ttgggatgag tgatgagatt
     3241 ggacacatcg ccgcgggcca cattgcagat ctcgtagcat gggacagtca tttgcgcgta
     3301 cggcgggtct ggcggcaagg tgttgagata tcgaacgtat ccagttacgg agaggtggaa
     3361 atcagcccat gaccgatcgt ttttcgctgg atgggaaaat cgctgtggtg accggcggca
     3421 gccggggaat agggaaaggt atcgctgtcg gatttgccga acatggcgcc gacgtcgcga
     3481 tagtgtaccg gtccgcacag aaggaggcgg aggcagtcgc ggaacgaatc cgggggctgg
     3541 ggcggcgatg ctgggtattt cgtcgcgacc tcggtgaggt cgaaacgttg tcagatttcg
     3601 tggatgatat atggcgtaca acgggacgca ttgatgtgct ggtaaacaac gcaggaatgg
     3661 catacctcga gaggttcaac gaaatcactg cagcgcattg gcgtcgcctg atggctgtca
     3721 atctggacgc cgtgtttttc atggcgcaac gcaccgcgga gcacatgatt cgcgacggca
     3781 tcaaaggccg catcatcaac ctatcatcga aaaacggact ggtcgccgag gcaggactgg
     3841 cacattataa cgcgtcaaag ggcggcgtgg aactggtgac caaatcgctt gccattgagc
     3901 tgggagaaca cggtatcacc gtgaattgca tcgccccggg aatcatcgaa acagagattg
     3961 cgggcgaatt cgacatcgac cccgcgttct tctcgtacta caaggaacac atcccgctcg
     4021 aacaccggtt tggtgccgta gaggagtgcg tcggcgcggc agtgtttctt gcatcttctg
     4081 ccgggtccta cattaccggt cagagtctgg tgatagatgg gggcgtgctg tgtgaacagg
     4141 tcccacggat gcagttcatg tccacgttca agaacacgtt gaacggtgga tgacggaatc
     4201 gccggtcggc aggcaggccc gcgaacacgc aacatgcgag cgctgaacgc agttcccgcc
     4261 gggaaggatg cggtggcagc gatttcccgg aaagggatca cagaaatggc gtaagaggct
     4321 gagtcaacag cctccgatct caatctgccc gagcaggatg tttagtgcgt cactccaccc
     4381 ggtaattcgg tgcttccttt gttatcgtca cgtcgtgcgc gtgactttcg cgcaaccccg
     4441 cctggcttat ccggataaag cgcgcctttt cgtgcaactc ggaaatcgtc attgccccta
     4501 cgtatcccat accggatcga agacccccga caagttggta cacgtaggca ctcaacgagc
     4561 ctttgtaagg cactctgccc tcgataccct cagggacgag cttttccggc tcgacatcgt
     4621 tctggaggta cctttcgctt gatccctgaa ccatagcgcc gagtgagccc atccctctgt
     4681 aaaccttgaa cgtgcgcccc tggtaaatga tcgtttcccc ggggctttcg tccgttccgg
     4741 caaaaagaga cccgatcatt atcgtgcttg caccggcggc gaggcctttg gtaatgtctc
     4801 cggaatgctt gactcctcca tccgcgataa tcgggatgcc gtattgctgc gcacactccg
     4861 cgcattcctg taccgcagtg atctggggta ctccgacgcc ggaaacgact cgcgtcgtgc
     4921 agattgaccc tggccccaca ccaactttga cgccgtccac cccggcatca atgagagctt
     4981 tcgtgccttc tctggtcgcg acatttcccg ccaccacagg cacattcggg aaccgcttct
     5041 tgaacgcctg aacggtggaa cacacgcgcc tggaatggcc acgcgcggtg tcaatcacca
     5101 ccacatccac ctctttttcc accagcgccg tggcccgcgc ctcccagtcc gcccccgtac
     5161 ctattgccgc cgcaacacgc agtctgccga gactatcggt gcacgcgttc gggtactgga
     5221 ttttcttctc gatatccttg acggtgatga gaccccggag gtacccctcg ctgtcgacaa
     5281 caagaagttt ttctatgcga tggcggtgca gggtagctcg ggcctcctcc atggatactc
     5341 cgaccggagc cgtgaccaga ttctcgctcg tcatcacgtt cctgattggc tgatcgagtt
     5401 tcctttcgaa ccgcaggtcc cgattcgtga gtatccctac gagcttgccg tgggggtcaa
     5461 ccacgggaac acccgatatg tgcagttcgg acatcagagt caatgcttcc cgaatggaag
     5521 cgtcgggccc cagggtgacg ggatcgacga tcattccgct ttccgagcgc ttcacctttt
     5581 cgacttcgga ggcctggtat tcaacgggag cgttgcggtg gatgacgcct attcctccct
     5641 cccgggcaag cgcgattgcg aggcgtgctt ccgtgacagt atccatcgcg gcgctgacaa
     5701 ggggaatgtt cacgggaacc tggcgcgtga acggcgcggt cgtcacaaca tctgctggag
     5761 caacgtcaga ttcaccggga acgagcagga catcgtcgaa tgtcaggcct tctgggatcg
     5821 catcgcgcat ggttccattt ctccgtgcgg cgttggggcg tcgcatcccg gggtggggtg
     5881 ttctgggtga attggtaaaa tatgccgcca tttcgtttgc gaagtcaaga catcgaaaca
     5941 ccggcgtctg agacgggcca tcacgaaaac tccaagattc acaaacaggc gcgcgttgga
     6001 agacaggtgt gtcacgcggt atattgaaaa acgttcaatg gaccacacac gcaacggctg
     6061 caggaacctg ctcactgaaa tacctcgcgc cgccccaccg aattgatcgg atttccgccc
     6121 ggtgagattg tacgttgtcc tcggacaccg ttcgtgaacc ggtagcagtg acggcatact
     6181 gcgtctggga ggtgaactca tgcacaccat catggacaag cgtttgcttg cgcccaatgt
     6241 gtacaggctc gaagtcggcg cacagttgat cgccgaaaga aggaaaccgg ggcagtttat
     6301 cattcttcgg gtgaacgagg agtcggagcg catcccgctt acaatcgtgg aaagcgaccc
     6361 tgtcgcggga acaatcgttc ttgtgttcca ggtgatgggc gagggtacgc ggttgctggc
     6421 ttctctcgaa atgggtgatg agattcggga cgttgtcgga cctttgggac atcccacgga
     6481 aatcagccac tacggcacgg tggtctgcgt cgccggagga atcggtgtgg cccccatgtt
     6541 tccgattgcc aaagggatgg ccgacatggc gaatacggtg atcagtatcg taggagcgag
     6601 aacgcgtgac ctcttgatac ttcaggagga aattcaatcg gtctcctcgg aggtgttgat
     6661 tactaccgac gacgggacct tcggccggca tgggtttgtc actgatgcat tgaaggaacg
     6721 cctcgagtca ggtttcacac cggacgctgt cgtggcgata ggccctcttc cgatgatgcg
     6781 ggcagtagcg gagacgactc gcccttacgg catcaagacc atcgtcagcc tgaacccgat
     6841 catggtcgac ggcaccggaa tgtgtggcgg ttgccgcgtt tctgtaggcg ggcagatgaa
     6901 gtttgcctgt gtagatggac cggaattcga cgggcatcag gtgaactacg cggaactggc
     6961 gaatcgtcaa cggatgtacc gtgataaaga ggcgtgcaga attcgaagtc tgataccctg
     7021 atctcccaac cccacctgtg gaacattgac gaagaactgc tcggaaaagg ctgccaaatg
     7081 aacccttcag aaaatgctgt tgaaaggaac cctgagcgac gcaaacctca acctgcgcgg
     7141 ccgcgacagg taatgcctga acgtccgccg ttggagagga accgggactt tgccgaggtc
     7201 gctaccgggt ttaccgccga aatggcaatc gcggaatcag aacgatgcct ccagtgcaaa
     7261 aaaccgctct gcgttcaggg gtgcccggta ggcattgaca tcccggcatt cctccgtttc
     7321 atcgctgacg gggatttcgc gggttcgata aacaggctga aggaggacac gaatcttccc
     7381 gcgatctgcg ggcgagtgtg ccctcaggag actcagtgcg aagaaaagtg catcgttggc
     7441 aaacggaacc aaccgatcgc gataggtgcg ctggaacgtt tcgtcgctga ttgggaaagg
     7501 gagaacggcg ccgtcaaaac acccgtagtt ggtgcaccga caggaaaaag ggtcgcggtg
     7561 gtaggttcgg gacctgctgg gttgacgacg gcaggcgaac tcgcacgtcg gggacacgcc
     7621 gtgactgttt ttgaagcgtt gcacgagccg ggtggagtgc tcgtctacgg catcccggag
     7681 ttccgtctcc cgaaagaaat cgtccggcgc gaggtggacg ggttgcgggc aatgggagtg
     7741 gagatccggc tcaacaccat cgtcggcaga tcccttacca ttgacgacat gttcgcgcag
     7801 gggttcgatg cggttttcgt gggctccggc gcagggctgc cacatttcct cggaatcccc
     7861 ggtgagaacc tcaacggcgt atacagcgcc aacgagttcc tcacccgcat gaacctgatg
     7921 aaagcgttcc ggttcccgga ctaccacacc ccggttcacg tcggcaaaaa gatcgcggta
     7981 ttcggtggtg gcaatactgc gatggatgcc gccagaaccg cactcagaat gggtcccgaa
     8041 tccgtgaaac tcatctaccg ccgttcagaa gcggaaatgc ctgcgcgtgc ggaagagatt
     8101 caccacggac gagacgaggg agtggagttt gttacgctga ctgcaccggt ggaatacctt
     8161 gacgacggga aagggtgggt ggccggtgtc aaaatcatca agatggaact gggcgaaccc
     8221 gacgcgagcg gaaggcggcg tccggttccg attgaaggaa gtgaagagat aatcgagata
     8281 gacactgccg tgatcgccat aggtaacagc cccaatcagt tgattccgca gacaacaaaa
     8341 gggctcgaga ccacacggca cggaacacta attgcgaatg aagccaccgg cgagacgacg
     8401 aaaaccggag tattcgcagg cggtgatgtc gtgacgggtg ccgctaccgt gatccaggcc
     8461 atgggcgcgg gaaaggccgc ggcggccgcc atagacgatt tcctgatgaa acgcgcgaac
     8521 gcggaagaac tcaggcagta ccgcgagaag ttatcggggt tcttccttgt agattcacct
     8581 cgacgaacgc cggaagaacg tcttgcgctc ctgcgctctc tcggcgtgcc cgaaccggaa
     8641 tcttttctcg ccaagcggtt gccgcaatgg gatgcacgcg tagacgaact gcttgatcct
     8701 tcgtcactcg acatgatgcc ggtccacgtt tcgcatgcac aggcgcggta tgtgcgaggt
     8761 gccatccaaa gccttccgga agacgtgaag gcgcgcattt tttccgcaaa aatgggcccg
     8821 acaggagttg gcgaggctat taggtcgctt tacctgcgcg aaaaaggcat ttcgctaccg
     8881 gaggatttca cagttgtttc ggtaactctg ctcgacgatc tgcgtcagtc cgttcgggtg
     8941 gtggttcagg acggagggag gagtctcacc gttgatgtcg tgcgcatgaa tgcggacgca
     9001 gagattgtgt attccgcagt cgcggaggaa ctcggaatcg ctcactgccg ggccgagatc
     9061 gtttttacgc ccaaaggtga caaagctgcg gtttcagtgg taccgagcgg cgcgaggctt
     9121 tcggatgagg ccgcactgag cgacgagtat ttc
//
LOCUS       DAOTNG010000109         9117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6936_length_9117_cov_5.163319, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000109 DAOTNG010000000
VERSION     DAOTNG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_9117_cov_5.163319"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="P5179_06755"
     CDS             complement(<1..830)
                     /locus_tag="P5179_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRS94951.1"
                     /translation="MRKHKRVYQVAKELHVSSQVLMSCCASLGIEVKTHMDPLEDEDL
                     DRLTKVFEKYPDGVIPAEPASDAPPEGTEAVPVVAAEPEPVAPAIPPAAPIPPPSRVF
                     MPPVDTAPIRPQLSTPAPAKPVEPAQEKPEPEKPAVQKERPALRPLSRPGVPDQQRPQ
                     RSFPPPAGSSALPPLRRPGATEAPRRPEAPARPERPALKPLRPRRSEGAEDISRADRL
                     RKMELENEDPEFAEWAKAKTIPLPGSGTIEPVADTGDVADAGLGDAAGVRRRRGKRRK
                     "
     gene            complement(889..2286)
                     /gene="nusA"
                     /locus_tag="P5179_06760"
     CDS             complement(889..2286)
                     /gene="nusA"
                     /locus_tag="P5179_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRS94952.1"
                     /translation="MNRDDVLEALRQVAREKNVTMDLVIDTLEEGLLSAAKKKFGLSD
                     NIDVEVDQNTGEIVIWAHKAVVEKVEDPAIEISLADAKKIDPEAAIGEGVAQPIPFTA
                     FGRMAIQSAKQMLIQKVREAERQRVYETFREAISDIRIGTAQQVLRGDVIVDLGRAEG
                     ILPVSEQIPRDRYRQKDTVRAYVKDVLKDTKGPQVILSRTHPGFLERLFQMEVPEVYD
                     GTVEVIAVARDPGSRAKVAVASRDDRVDPVGACIGQKGMRIQQVVRELNNERVDIIEW
                     SPDPVVFFQRSLAPAEIQRVEVDQERQTMIAIVDEDQLSIAIGRGGQNARLAAQLTGW
                     RVEIMTEEQYAKKKERDEFLRVPVSELPGSTPAVAKALTEAGIGSALELSERSAVEIA
                     DIAGISAERAAQLLDEATDLLEAKRQEYAERLQIPLEETGVDLATFVAEPSYGLAATD
                     EPGIAETSPEAPGLT"
     gene            complement(2283..2813)
                     /locus_tag="P5179_06765"
     CDS             complement(2283..2813)
                     /locus_tag="P5179_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94953.1"
                     /translation="MQQEERDSVQNVATAIAGPMGVEVLEIKWLGSGRTRSVRLVVDQ
                     EGGVGADTCARLSREFDLEWEARTATPRDFALEITSPGPDRPLRTVKDFLRVVNRWVQ
                     IERQSDAGTDTLTGKVVSCSEDALVLSESGIFPDAGDLSGCVAVPFAEIRNAKILFVI
                     GNTTPRGKMARRTTRR"
     gene            complement(2915..3439)
                     /locus_tag="P5179_06770"
     CDS             complement(2915..3439)
                     /locus_tag="P5179_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRS94954.1"
                     /translation="MTDLPALAHARAIAGRACALLVGLAASGCGVYSVEGGLPSHVRT
                     VGVEFFENSTTEAGVEERLSRAISDQLVTRSQVRYAPFSGADAIVRGSVTRVNEEPLT
                     FSGTQVSRYRVTVLVDASVWDRARRRNLWEREGIQGQGQYDATAGVAGRDAAYASAIQ
                     EAARQVVDGFLSGW"
     gene            complement(3436..4461)
                     /gene="mtnA"
                     /locus_tag="P5179_06775"
     CDS             complement(3436..4461)
                     /gene="mtnA"
                     /locus_tag="P5179_06775"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRS94955.1"
                     /translation="MKIKTLEWVGDGLRLLDQTLLPNDERYIVCTDVEVLAHAIETLQ
                     VRGAPAIGVAAAYGVVIAAMNAGPSHRREAVAAAIARLRRTRPTAVNLFWALDRMRSV
                     ADVLADGDLVTGLLKEAQAIEVDDTRRCEAMGRHGAALLPDEATVFTHCNAGGLATSG
                     YGTALGVFYAAAEMGKKLRVFADETRPLLQGARLTAWELSKAGIDVTVICDNMAAAVM
                     RQTKVDGVFVGADRIAANGDAANKIGTYGLAIIARHHNVPFYVVAPMSTVDISIPDGS
                     GIPIELRNENEVRRGFGPNTVPESAHVFNPAFDVTPADLISGIVTEFGIARPPYSATL
                     KTWLEAV"
     gene            complement(4458..5396)
                     /locus_tag="P5179_06780"
     CDS             complement(4458..5396)
                     /locus_tag="P5179_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS94956.1"
                     /translation="MPDSLLIVGSVAYDNVETPGGRVENALGGSALFGAAAASLFAPV
                     HLVGIVGTDFDRAGIAFLEERGVDLAGLETAEGKTFRWTGVYEEDMNNRRTLATELNV
                     FADFAPRIPDGYRGDKVVFLGNIAPRLQLHVLDQMAGPDWVALDTMDMWIRDAADDLA
                     RVLQRVHMVVINDSEARALTGKTNLTVAARSILDMGPRAVVVKKGEHGVLLAAEDEFS
                     VLPALPLKTVVDPTGAGDSFAGGMLGYIAATGDFTFSGLRRALAYGTAAASLAVEDYS
                     FNRLRNATREVLDERYRWLRSISFLPDLHGQERHNA"
     gene            complement(5515..6687)
                     /locus_tag="P5179_06785"
     CDS             complement(5515..6687)
                     /locus_tag="P5179_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94957.1"
                     /translation="MSEGQKCLDLGIVGLGECGGNLAVEFTRLGYKAVAVNTSHTDLR
                     ALSLDPRKRIYIGYEGRDGAGQDMALGQRSLEASSDIIVEAVGTQLEGCRHLLLCAGL
                     GGGTGSNIAVLANILARLDIPISALASLPKDHESSIAKINAVNAINLFRNSDVASIIL
                     VDNEKILRQYRSESLTAFYSSANQAAVERLHEMNTLSTNELYMPIRGFDGEDFRRVFA
                     SRGVLIYGAADLTDDDLLIRDRLANGLRSIWDSSGLLASGFDYKDATMAGIILVAPQD
                     LLDRAPADIFESLIRQIRELTDTSGIYAGLFQGPPDQMPRLYTMLGGLPFPNRLRTVL
                     GQAKEEGPALGVKVARPIEELDLGELSGLDLFSAGYIGPVPAGVGGQASPAERRPR"
     gene            complement(6702..7160)
                     /gene="rsfS"
                     /locus_tag="P5179_06790"
     CDS             complement(6702..7160)
                     /gene="rsfS"
                     /locus_tag="P5179_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRS94958.1"
                     /translation="MIQKKARSRRKPARDAAPVPETVGVLVEAVRSKKASNVVVMDLR
                     GLTDVADFFVLCTADVDVHARAILDAVREALGPFGRDPWHVEGEESLNWVLVDFVDTV
                     VHIFREDARQFYGLEEMWADAPRVPFPDVPPRVGETLHAGSLPPADNPRS"
     gene            complement(7204..7707)
                     /locus_tag="P5179_06795"
     CDS             complement(7204..7707)
                     /locus_tag="P5179_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94959.1"
                     /translation="MKTVAILVAGVLALAAQTATASGPFVTNTGERGPTIAGAPTADL
                     VSLMRRQGLFDSSKLTTWRSYSFGIASSGGTTTSAGLLLQHIQYQIATPLTLRMDVGL
                     LHNPLGVAGFRNGPQQASIVIPALDLVYRPTENMVLSVHYSQMPSSRYGYGYGYNAWW
                     NDPSTGW"
BASE COUNT         1751 a         2961 c         2672 g         1733 t
ORIGIN      
        1 ttcttcctcc gtttcccgcg tctgcgccgc acacctgccg cgtcgcccaa gccggcatcg
       61 gcgacatcgc ccgtatccgc aaccggttcg atcgtgccgc tacccggaag tggaatcgtc
      121 ttggctttcg cccattcagc gaattccgga tcctcgtttt ccagttccat tttgcggagg
      181 cgatcggcac gcgagatatc ctccgcccct tccgatctgc ggggtcggag cggcttcagc
      241 gcagggcgct ccggccgcgc gggggcttcc gggcgcctcg gtgcttcggt ggcgcccggc
      301 ctccgaagag gcgggagagc cgatgaacct gccggcggcg gaaacgatcg ttgcggacgc
      361 tgctgatcgg gaacgccagg acgggacaac ggcctcaacg caggacgttc tttctggacg
      421 gccggttttt ctggctccgg tttttcctgc gcaggctcaa cgggttttgc cggggccggt
      481 gtgctcaact gcggtcggat cggagccgta tcgacgggag gcatgaacac gcgcgaaggc
      541 ggcggtatcg gagcggcggg aggaattgcc ggcgcaacgg gttccggctc ggccgcaacg
      601 accggaacgg cttctgtccc ctcaggtgga gcgtcgctgg caggttccgc aggaattacg
      661 ccgtcgggat atttctcaaa taccttggtc agacgatcga gatcctcgtc ctccaacggg
      721 tccatgtgcg ttttcacctc tataccgagc gaggcgcaac agctcatcag aacctgtgaa
      781 ctgacgtgga gctctttcgc tacctgataa actcgtttgt gctttcgcaa cgcgtacgtt
      841 cctcccgaaa ttcggcatcc ttacacagga agggcaacat tagtcctact acgtcagacc
      901 cggggcctcc gggcttgtct ccgcgattcc cggctcatcc gttgctgcca ggccgtagga
      961 gggctccgcg acgaacgttg ccagatccac gcccgtttct tccagcggaa tctgaagccg
     1021 ttccgcatac tcctgccgtt tggcttccag caagtcggtt gcttcgtcca gcaactgggc
     1081 cgcccgttcc gccgaaattc cggcaatatc cgcgatctcc accgcggaac gttccgaaag
     1141 ctccagagcg gagcctatac cggcttcggt gagagccttg gcaactgccg gtgtcgaacc
     1201 gggaagctcc gagacgggaa cacgaaggaa ttcgtcgcgc tccttcttct tcgcgtactg
     1261 ctcttccgtc atgatctcga cgcgccaacc ggtgagttgc gccgcgagac gcgcgttttg
     1321 gccgccgcga ccgatcgcga tggagagctg atcctcatca acgatcgcga tcatcgtctg
     1381 gcgctcctgg tcaacttcga cgcgctgaat ctccgccggt gccagagagc gctggaaaaa
     1441 tacaacagga tccggagacc attcgataat gtcaacacgc tcgttgttca gttcgcggac
     1501 cacctgctgg atccgcatgc ctttctgacc tatgcaggcg cccaccggat ccacacggtc
     1561 atcgcggctg gcaactgcca ccttggcgcg gcttccggga tcgcgagcaa ccgcgatcac
     1621 ttcgacagtg ccgtcgtaga cttcggggac ttccatctgg aacaaccgtt cgaggaagcc
     1681 gggatgggtg cgggaaagga tcacctgggg tcccttggtg tccttgagaa cgtccttcac
     1741 gtaggcacgg accgtgtcct tctggcggta gcgatcgcgg ggtatctgct cggatacggg
     1801 cagaattcct tccgcgcggc ccagatctac gatcacgtcg ccacgaagca cctgctgtgc
     1861 agttcctatc cggatatcac tgatagcttc gcggaacgtc tcgtacaccc gttgtcgctc
     1921 tgcttcgcgc actttctgga tgagcatctg cttcgcggac tggatagcca tcctcccgaa
     1981 cgcggtgaag ggaataggct gtgccactcc ttcgccaatc gcggcttccg gatctatctt
     2041 cttcgcgtca gccagggaaa tctcgatggc gggatcctcg accttttcca ccaccgcctt
     2101 gtgcgcccag atgactattt cacccgtatt ctgatccact tccacgtcga tattgtcgga
     2161 aagaccaaat ttcttcttcg ccgctgaaag cagcccctcc tccaacgtat cgatgacgag
     2221 atccatcgta acgttctttt cgcgggctac ctggcgtagt gcttcgagca catcgtcacg
     2281 attcatctgc gtgtcgttct ccgtgccatt ttgcctctcg gagtcgtgtt tccaatgaca
     2341 aaaagaattt tcgcgttgcg tatttccgcg aacggaaccg caacgcagcc tgaaaggtcc
     2401 cccgcatcgg gaaagatccc gctttcggac aggaccagcg catcttcaga acaggacacc
     2461 actttccctg tcagcgtatc ggttcccgca tcggactggc gttcgatctg aacccacctg
     2521 ttgacgaccc gcaagaagtc cttcaccgtg cgaaggggac gatcgggacc cggggaagtg
     2581 atttcgaggg caaaatcccg cggcgtggcc gtccgcgctt cccactcgag gtcaaactcg
     2641 cggctcaggc gggcgcaggt gtctgcccct actccgcctt cctgatccac aaccagtctg
     2701 accgaccgtg tccttccgga accgagccac ttgatttcca gaacttccac acccatcggg
     2761 ccggcaatcg cggtcgcaac gttctggacg gaatctcttt cctcttgctg cacaaagcgc
     2821 cccaatatga aaagagtgag cgcgcgccca ctcataaatc atatgaaggt acgcattttc
     2881 gcggtcgaac gcaacgacgc gtgcgtaggg agtcctacca gccgctgaga aacccgtcga
     2941 ccacctggcg ggccgcttcc tgaatcgccg aggcataggc cgcatctctc cccgcaaccc
     3001 ccgccgtcgc gtcgtactgg ccctgcccct ggattccttc ccgttcccac agatttctgc
     3061 gccgggcgcg atcccagacc gaagcgtcca ccagaaccgt cacgcggtaa cggctgacct
     3121 gcgtcccgga aaaggtgagc ggctcctcgt ttacgcgcgt gacacttccc cgcacgattg
     3181 cgtcagcacc ggaaaacgga gcgtaacgaa cctgcgacct ggtaaccagt tgatccgaga
     3241 tggctcgcga aaggcgctcc tctacgcccg cttccgtcgt cgagttttcg aaaaactcga
     3301 cgccaaccgt tcttacgtgc gagggcaatc cgccctccac ggagtacaca ccacatcccg
     3361 aggcggccag ccccaccaat aaggcgcatg ctctgcccgc gattgcacgc gcgtgcgcaa
     3421 gtgcgggcaa atccgtcaca cagcctccaa ccatgttttg agcgttgcgg agtagggagg
     3481 acgggcaata ccgaactccg tgacaatccc ggagatcaga tcggcaggag taacgtcgaa
     3541 cgcagggttg aacacgtggg ccgattcagg aacggtattc ggcccgaacc cgcgtctgac
     3601 ctcgttctca ttccgcaact cgatgggaat cccggaacca tccggtatcg agatgtcaac
     3661 ggtagacatg ggggccacca cgtagaacgg cacattgtgg tggcgcgcga tgatcgcgag
     3721 gccgtacgtg ccgattttgt tcgccgcgtc cccgtttgca gcaatgcgat ctgctcccac
     3781 gaacacgccg tccaccttcg tctgccgcat cactgccgcg gccatgttgt cgcagatcac
     3841 cgtcacgtcg atcccggctt tcgaaagctc ccacgcggtc aggcgagcac cctgcagaag
     3901 aggcctcgtt tcgtctgcga atacccggag tttctttccc atttcagccg ccgcatagaa
     3961 cactccgagc gcggtgccat accctgacgt agccaatccc cccgcattgc aatgagtgaa
     4021 cacggttgcc tcgtccggca gcagcgcggc gccatggcgg cccatcgcct cacatctgcg
     4081 agtgtcgtca acctcgatgg cctgcgcctc cttgagcaac cctgttacaa ggtccccgtc
     4141 cgctaatacg tcggcgacgg acctcattcg atccagagcc cagaaaaggt tcactgccgt
     4201 cggacgcgtt cgccgcagac gagcgatggc cgcggccact gcctcgcggc ggtgcgaagg
     4261 gcctgcgttc atcgcagcga tcacaacccc gtacgccgct gccacaccta tggccggagc
     4321 accacggacc tggagcgttt cgatggcgtg ggccagaacc tcgacgtcgg tacagacgat
     4381 gtatcgttcg tcgttcggga ggagcgtctg gtcaagcagc ctgagtccgt cgcctaccca
     4441 ttcaagtgtc ttgatcttca tgcgttgtgg cgctcctgac cgtgcagatc gggaagaaac
     4501 gaaatagacc gaagccagcg gtaccgttcg tcaagcactt cgcgtgtggc atttctgagc
     4561 cggttgaagg agtaatcttc cacggccaga ctcgcggcag ccgttccgta ggcaagcgcc
     4621 cggcgcaaac cggagaaggt gaaatcgcct gtcgcggcga tgtacccgag cattcccccc
     4681 gcgaaggaat cgccggctcc cgtggggtca accaccgttt tcagtggaag cgcggggagg
     4741 acggagaact cgtcttccgc cgcaagaagc accccgtgct cgcctttttt cactactact
     4801 gcacggggac ccatgtccag aatcgaacgg gcggcaaccg tgagattcgt cttccccgta
     4861 agggccctgg cttccgagtc gttgatgact accatgtgga cgcgctgcag cacacgggca
     4921 aggtcgtctg ccgcatcacg gatccacata tccatcgtgt ccagcgccac ccagtccgga
     4981 ccggccatct ggtcaagcac gtgcaactga agccgcggcg cgatgtttcc gagaaacaca
     5041 actttgtcgc cgcggtaccc gtcaggaata cgtggtgcga aatccgcaaa gacattgagt
     5101 tccgtcgcga gcgtccggcg gttgttcatg tcctcttcgt acacgcccgt ccaccggaat
     5161 gtcttgccct cagcagtttc caaacctgcc aggtccacgc ccctttcctc aaggaacgcg
     5221 atcccggcac ggtcgaaatc agtccccaca atccctacga gatgaaccgg ggcgaacagc
     5281 gacgccgcgg cagcaccaaa aagggcggaa ccgccaagcg cgttttccac tctgccaccg
     5341 ggcgtttcca cgttatcata cgcgactgaa cccacgatga gaagcgaatc aggcatagtc
     5401 gttttccttg acgttcaaac gtacacgcag aagccgcagg agctgcccgg acagacctgc
     5461 ggcttccagg aacacactaa atccggctgt ctgcggcgag gtccgcgaaa caaactaccg
     5521 gggacgcctc tcggcgggcg atgcttgtcc gccgacgccc gccggaaccg gcccgatgta
     5581 ccccgcggag aacagatcga gcccggaaag ctccccgaga tcaagctcct cgatcgggcg
     5641 cgcaactttg acgcccagcg caggcccttc ttccttcgcc tgcccgagaa ccgtgcgcaa
     5701 ccggttgggg aacggaaggc ctccgagcat ggtgtacagg cggggcatct ggtcgggagg
     5761 cccctggaaa agaccggcgt aaataccact tgtatcggtg agttcacgaa tctgacggat
     5821 caggctctcg aagatgtccg ccggcgcacg atcgagaaga tcctgcggcg ccacaaggat
     5881 gatacccgcc attgtcgcgt ccttgtagtc gaaaccggag gccagaagtc ctgaagaatc
     5941 ccagatgctt ctcaggccgt tggcaaggcg atctctgatc agcaggtcgt cgtccgtgag
     6001 atccgcggcc ccgtagatga gcacgccgcg cgaggcaaac acgcgccgga aatcctcccc
     6061 gtcgaaaccg cgaatcggca tgtacaactc gttggtgctg agcgtgttca tctcgtgcag
     6121 gcgttccacc gccgcctgat tcgcggagga gtaaaacgcg gtcaggcttt cacttcggta
     6181 ctgacgcaga atcttttcgt tgtccacgag aattatgctg gcgacgtccg aattgcggaa
     6241 aagattgatg gcattgaccg cgttgatctt cgcgatactg ctctcgtggt ctttcggaag
     6301 agaagccagc gcggaaatag ggatatccag ccgcgccagg atattcgcaa gcacggcgat
     6361 attgctgccc gtgcctccgc caaggcccgc gcacagaagc aggtgtctgc acccttcaag
     6421 ctgggtaccc accgcttcca caatgatatc gctgctcgcc tcgaggctcc gctgacccag
     6481 cgccatgtcc tgaccggcgc cgtcgcgccc ttcgtacccg atgtagatgc gttttctggg
     6541 gtccagcgac agcgcgcgaa ggtccgtatg tgaagtgttc accgcaacgg ctttgtaccc
     6601 gagccttgtg aactcaacag ccaggttgcc gccacattcg cccagtccga cgatgccaag
     6661 gtccaaacac ttctgccctt ctgacatcta ctcctcctct ttcacgatcg cgggttatcc
     6721 gcaggcggga ggctccccgc gtgcagggtt tccccgacgc ggggcggaac gtccgggaag
     6781 ggaacgcgcg gcgcatccgc ccacatttcc tccagaccgt agaattgacg tgcgtcctct
     6841 cgaaaaatat gcaccaccgt gtccacgaaa tctacgagca cccagttgag ggattcctcc
     6901 ccctcaacgt gccacgggtc acggccaaat gggcccagag cctctcgcac cgcatcaagg
     6961 atggcgcggg catggacatc gacgtccgcg gtgcacaaca cgaaaaaatc cgcgacgtcg
     7021 gtaagccccc gcagatccat gactacaaca ttcgacgcct tcttgcttct gaccgcctcc
     7081 acaagaaccc cgaccgtttc gggaacgggg gcggcatcgc gtgccggttt ccttcgtgaa
     7141 cgcgcctttt tctgtatcat tcaacttccg gttcaggtcc gctcgcgccc gttcgggacg
     7201 gcatcaccat ccggtggacg gatcattcca ccacgcgttg tacccatagc cgtacccgta
     7261 ccggctggac ggcatctgcg agtaatgaac ggacagcacc atgttttccg tcggccggta
     7321 taccagatcg agcgccggta tgacgatgct ggcctgttgg gggccgttgc ggaatcccgc
     7381 cacacccagc ggattgtgca acaaccccac atccattcgc aaggtcagcg gggtggcaat
     7441 ctggtactgg atatgctgaa gcagaagccc tgcggatgtc gtggttcccc cgctgctggc
     7501 gatcccgaac gagtaggaac gccacgtcgt caacttgctg gaatcgaaca acccctgacg
     7561 tctcatcagg ctgacgagat ccgcggtggg cgcgccggct atcgtgggcc cccgctcgcc
     7621 ggtattggtg acaaacggcc ctgacgccgt ggctgtctgc gccgccagag caagaactcc
     7681 cgccacaaga atcgctacag ttttcatcgc tcgtatcctt ctctccgggg tgtggcaccc
     7741 atgcccttca accccttcct gtgaacgcac ggcaaaccca gcctgttccg cgcatacaac
     7801 aggtcatcaa agaataacaa tctccgcggc aaactcaaat acgctgcgtg cacccgcgga
     7861 ggtcgtaacg acgcacgctc ccacgccggg acaggcggct ccgcacaccc gcgttccgat
     7921 gaactcatac gatgccccgt tcgatgcggt gcgggttcca gaatctgcac ggtgcccccc
     7981 cgattggctc agccgtgagg cggatttcag agtttgcccc gtgacgtact ggctgaggca
     8041 aagagtcctg ttcgtgtcaa ggcttttcgc gaaaatgttt caatcggggt acggtttttt
     8101 ttctgggggg tggaggggca cgcttcgtcg tgcctgccga ccagcggctg cgtgccgccg
     8161 gacggctgcc ttgaagcgcc aacggcgcgc gatagcggag cccggttcgc aggaccgggc
     8221 gagggggtgc ctgcatggac ccgacggagc gggtccctcc agggccggtg gtcaggtctt
     8281 cgtggacggg gtggacagag tggaccgggc tggcgcaagg gccgggcggt gagatgatga
     8341 acggtcatct cgatacaacg aaccggcgga gaacgtcgcg ttgcgtcccc cctccccccg
     8401 ccggttcgcc actcgatgac cggtgagggt cgagtcgttg gacatcaacc gacagtcaaa
     8461 acgcggcgcg aacccgacgg agcgggtccc tccagacggg agggtgcgtg tggggcacac
     8521 gcgcgccggt ctccgagtgg cggacgcgaa gcggacgctg tatcgaggag cccgtcgcgc
     8581 agcaccaggt gagggcgtgc ctgcgcggac ccgacagttg gaacgcggcg tggacctgac
     8641 ggagcgggtc cctccagggc acccggccct ttgagccggg ctggactata gcggcccttc
     8701 aggccttggt ggacgcgcaa ggattcttga agcgccgacg gcgcgcgata gcagagcccg
     8761 gttcgcagga cggggcgagg gcgtgcctgc gcggacccgg cagttagaac gcggcgcgga
     8821 cccgacagag cgggtccctc cagacgggag ggtgcgtgtg gggcacacgc gcgccggtct
     8881 ccgagtggcg gacgcgaagc ggacgctgta tcgaggagcc cgtcgcgcag gtcgcccggc
     8941 caacccggcc cgggggggcg aaagaaaaag gggcgacctg cgtgcgaaat tccgcacgga
     9001 ccgatcgccc ctgctcaaga tgttctcgaa aggtttaccg cacgatggtc acccggctca
     9061 gaagcctgct ggtgggggtc tgcaggacgc agatatacac gccgctggca agctcac
//
LOCUS       DAOTNG010000110         9064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6982_length_9064_cov_4.392607, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000110 DAOTNG010000000
VERSION     DAOTNG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6982_length_9064_cov_4.392607"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="P5179_06800"
     CDS             <1..1080
                     /locus_tag="P5179_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94960.1"
                     /translation="FLEHDMDAVILANYFHEHAPFAIKALAAGKHVMSECICNATLGE
                     GVALCRAVEKSGLIYMLAENYPYTAFNMEMRRVYQTGEIGRVTYAEGEYNHPMAPEDR
                     LRIAPGMNHWRNWIPSTYYCTHALAPLVFITDTMPVMVNGLSIACREPDTKTVRRGDP
                     GSVILCRMDNGSVFRIFGLTLPGHSNWYRVHGTKGAMEITRGPGYYGPGQVRVWHEEW
                     DMKPGELRDRIYLPDWPVHADLARKAGHGGGDFWTNFEFANAIRSGTQPFLDVYRGVA
                     MSSVGILAWKSALKDGMPLEVPDFRNETKRTKYAKDNASPFPSHAGKKGYTRLPASIA
                     GDLKPTKEGLALAKRVWKEIGYKGD"
     gene            1152..1724
                     /locus_tag="P5179_06805"
     CDS             1152..1724
                     /locus_tag="P5179_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HRS94961.1"
                     /translation="MPVVLWRHFYHITHRDNLRNIFRYGILSWHCAHQRGLTASDISD
                     PQVQERRNRIEPVFRRSIHEYAPLYFNPKNPMLFRRRELQEELVILEVSPDVLRYNQH
                     IFTDGNAAAATTTFSTNREIIAASEDALRAEWWTALPDGKRRRCAEVLIFPSVTPRFI
                     TNAICANQGSASAINWTCPVSVIVDTSFFF"
     gene            1737..2528
                     /locus_tag="P5179_06810"
     CDS             1737..2528
                     /locus_tag="P5179_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014978934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRS94962.1"
                     /translation="MADISVIDGNIFTSRAQTLVNTVNCVGVMGAGIALECRLRYPEM
                     HDQYVSLCRRRMIEIGSLWIYKAPDRWILNFPTKRHWKEPSKEEYLHAGLRKFMQTYQ
                     EKQIESIAFPLLGAQHGGLRRDRSQELMESYLSECRIPIEIYRYDPMAPDDLYDRFCE
                     LLRDMTPEEMKVETGLRSDFVKRLLDAVHDPQVRQLNRLASLDGIGIGTLEKVFAFAR
                     GRILGRSQPVQQALEFGRVAEPTATFDHKNCSSYREGRKGKHIVG"
     gene            2895..3542
                     /locus_tag="P5179_06815"
     CDS             2895..3542
                     /locus_tag="P5179_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94963.1"
                     /translation="MYQNVAELNRDCEDYSKWWRAVDQELYRYFAEHSDHGNLRSVYA
                     KVAMVDRLYTTGLERARKGTVEIDRAQKVAELLFARGEELDCGIRMISGVPCLTRDAL
                     ADIAALHGWIAETCLKEAGVGAPVSFVSKYLHFHVPVVPIYDQQACASLARYIRGRFG
                     RYVATPERLPKLDGQMPAYRWYCARFAELWEEAQESRPETTVKELDYHLWVLGKT"
     gene            3594..>5073
                     /locus_tag="P5179_06820"
     CDS             3594..>5073
                     /locus_tag="P5179_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94964.1"
                     /translation="MLRYSVPGDEFQLDFRLVEAGEPVTFTLRGVTSRDRFEIFPRYL
                     ETCDPERARLSPAPLAWLDELGPVELPAGEVIRYTPEVPGNYLARWTSRQYGVEYRYF
                     AAVDQSYVIYRPAVWNWPTPFPASGGADIHNGGLPLDWCISSDSTDEPYVPRLRDEQQ
                     RYGGGVVYGMNTASADGPIDGAVLALRNTVANLRQHGLDVGRVANLWYGGGLSHDRVA
                     LARAAGFDVIDGYVPRAASCGLGAPYFPFYIGPVDYRFPGQHGPTDTLAFVFDFVGSW
                     HFHGPIGFHRPTANGSWERARHYIDLAAREASLNAKNSGAHNVITTLVNFESPIAWGN
                     PAYQVVWDEGRGRDFFDNYMHLLAFEHTRRWPIVFARAADYADYFRAHYTGMPRRIVS
                     SITHDIEYDRYWTDEWHEKGLTPAGYVPVNQPLRAFREARAMPQYNMPVSHEFMNYND
                     NRRTCRFEYACPKPLHYYDLTGAEPWPQQPAETDIPDPDITIS"
     assembly_gap    5074..5173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5174..6463
                     /locus_tag="P5179_06825"
     CDS             <5174..6463
                     /locus_tag="P5179_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS94965.1"
                     /translation="YSLLTGAYPWRNPRAAILTGDAPIIIEPGSPTLPGMLQRAGYRT
                     GVVGKWHLGLGQGDLDWNRDISATPNDVGFDHSFIMPATNDRVPCVYLENRRVVNLDP
                     DDPIEVTYADKKVFPGELTGREHPELLRVTHSHGHDGTIVNGVGRIGHMRGGRSALWT
                     DETMAETFAKKAVEFIAANREQQFFLYYALHQPHVPRLPNPRFKGATKLGARGDVIVE
                     MDWCVGEMLNALDRLGIRENTIVIFSSDNGPVLDDGYRDGAEELRDGHRPAGPLRGGK
                     YSLFDGGTRIPFILSWPGSVQPGESAALVSHVDFHASFAALTGQQLGHEEAPDSLNVL
                     DALLGRSGTGREELVVEGTRSKTVYRRGNWTYIPPYQGPQVNVNTNTELGNSLDPQLY
                     DLSQDIGQITNLAAAFPARAAEMQARLDSIRQGTCSR"
     gene            6641..>7257
                     /locus_tag="P5179_06830"
     CDS             6641..>7257
                     /locus_tag="P5179_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94966.1"
                     /translation="MYSPRALAFGLLLLAFPLLANATPVTGNLSTQTWTKTRSPYRVT
                     GKVTVLSGETLTIEPGVDVLFDADVQFHVRGRILALGAPLDSVRFLKGSAARWGGMRI
                     IKADSCVFHYVRFSDSHRRDASDKWEDISGGAVFVDSSVAVTMANTVISRNVADGYGG
                     GICIAHTKVTLTDCTIADNSAAEYGGGLSNNYATASLTMTNCLVVG"
     assembly_gap    7258..7357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7358..8652
                     /locus_tag="P5179_06835"
     CDS             <7358..8652
                     /locus_tag="P5179_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRS94967.1"
                     /translation="SNNYATASLTMTNCLVVGNTAVKYGGAIENWGAKTEILGCVFFG
                     NTATASGGTSDGSMLVNWGTSPTVTIRNSIVWGNAPVNAIRVDAGTVTATYSDIQRSS
                     GTFAGTGNINLNPQFVNTGVRNFRLLPASPCINTGDPGSPLDADGSRADMGITFSPEI
                     ILYGDVSGDGTVSAHDAALVVQRVVNSSLALARPFAGDVTGNGSLSAYDAALILRKVT
                     NPGYRFPVEGGTLKPALVSREPRRVHFERSGPCWVLEMDDPAGVVAGDLVLLLPDEAS
                     ARITGAVMSSFRQEGRSLHVSFVRADDGSPVLISVLTDAKTAPEIVRASFNEGAVTGV
                     ASQPVQFVLEQNFPNPFNPTTVVRFAIPEAGDVRLSIHAANGQLVRTLVDRPLPSGNH
                     EVVWDGKDALGREVASGVYVCRLRTERAEATRRMTLLR"
     gene            8830..>9064
                     /locus_tag="P5179_06840"
     CDS             8830..>9064
                     /locus_tag="P5179_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94968.1"
                     /translation="MNTCPVLKRARAVAALVGLIAVSGGCTPQTRAARVPEQDLTPFE
                     QLVRQWTREYRFRRTIGGSDLVSSQGAIVERPTH"
BASE COUNT         1839 a         2636 c         2715 g         1674 t
ORIGIN      
        1 ttcctcgagc acgacatgga cgcggtcatt cttgccaact actttcacga gcacgcgccg
       61 ttcgccatca aggcccttgc ggcgggcaag cacgtgatga gcgagtgcat ctgcaacgcc
      121 acgctggggg agggggtggc tctctgccgt gcggtggaga agagcgggct tatctacatg
      181 ctggcggaga actaccccta caccgccttc aacatggaga tgcggcgggt atatcagacc
      241 ggggagatcg gccgcgtcac ctacgccgag ggggaataca accatccgat ggcgccggaa
      301 gaccgcctgc gcatcgctcc cggcatgaac cactggcgca attggatacc atccacgtac
      361 tattgcaccc acgcgctcgc cccgctggta ttcatcactg atacgatgcc ggtgatggtg
      421 aacgggctgt ccatcgcgtg ccgcgaaccg gatacgaaga ccgtgcgccg cggggacccc
      481 ggctcggtaa tcctttgccg catggataac ggatcggtgt tccggatttt cggcctgacg
      541 ctgccgggac acagcaactg gtaccgcgtg catggcacca agggggcgat ggaaatcacc
      601 cggggacccg gctactacgg tcccgggcag gtgcgggtct ggcacgagga gtgggacatg
      661 aagccggggg aactcaggga tcgcatatac cttcccgact ggccggtgca cgcagacctc
      721 gcccgcaagg cagggcatgg cggcggcgat ttctggacga acttcgaatt cgccaacgcg
      781 attcgctcgg gcacgcagcc gttccttgac gtctatcgcg gcgtggccat gagcagtgtc
      841 ggcatcctcg cgtggaagag cgcgctcaag gatggtatgc cgctggaagt gccggatttc
      901 cggaacgaga caaagcgcac gaagtacgcg aaggacaacg cgtcgccatt cccctcgcat
      961 gccggcaaga aggggtacac acgccttccc gcaagcatcg ccggcgacct gaagccgacg
     1021 aaagaaggac tcgcgctggc gaaacgcgtg tggaaagaga tcggatataa gggggattga
     1081 gggcggggca aaccggcccg tcattcggaa acaaagactt gggtctggaa gtttcgcaga
     1141 aagggactca gatgccggtt gtattatgga gacatttcta ccacattacc catcgggata
     1201 acctacgcaa catcttccgg tacgggatcc tttcatggca ctgcgcacac caacgcggac
     1261 ttaccgcgag tgatatctca gatccccaag ttcaagagcg gagaaaccgt atcgaaccgg
     1321 tcttcaggcg ttccatccat gagtatgccc cgctgtactt caaccccaaa aatccgatgc
     1381 tattcagaag gagagaactt caggaggagc tcgtgatatt ggaggtgtcc ccggatgttc
     1441 tgcgatacaa ccaacacatc ttcaccgacg gcaacgccgc agcagcaaca accacctttt
     1501 ccactaaccg cgaaatcatt gcggcatcgg aagatgcgtt gcgggctgaa tggtggaccg
     1561 cgctcccaga cggtaagcgt cgccggtgcg cagaggtttt gatcttcccg agcgtcacac
     1621 cgcgcttcat aaccaacgcg atctgcgcaa accaaggctc cgcatcagcg ataaactgga
     1681 cctgtcccgt gtccgttata gtggacacgt cgtttttctt ttgaagggaa cggctcatgg
     1741 cggacatcag cgttatagat ggcaacatct tcacctcgag ggcgcagacg ctcgtcaaca
     1801 cggtaaactg cgtcggagtc atgggtgcgg gcatagcgct ggagtgccgt ctacggtacc
     1861 cagaaatgca cgaccaatat gtttctttgt gcagacgccg catgatcgaa atcggctcgc
     1921 tctggattta caaagctccc gacagatgga tcctcaattt tccgacgaag cgtcactgga
     1981 aggaaccgag caaagaagaa tacttgcacg ctggtttgag aaaattcatg cagacatacc
     2041 aagagaaaca aatcgaatca atcgcttttc cccttcttgg tgcccaacat ggtggccttc
     2101 gcagagatcg ctctcaagag ctgatggaat cgtatttgtc tgaatgtaga atcccaatcg
     2161 agatttaccg atacgatcca atggctcctg acgatctgta tgatcggttt tgcgaattgc
     2221 tgagggacat gacacccgag gaaatgaagg tggaaacggg acttcgttct gattttgtca
     2281 aaaggttact tgacgcggtt catgatcctc aagtccgtca actcaaccgt ctcgcatcac
     2341 tcgacggcat cggaatcggc acattggaga aggtgtttgc ctttgcacgt ggtcggattc
     2401 tcggtcgcag tcaacccgta caacaggccc tcgaattcgg aagggtcgcg gaaccaacgg
     2461 ctacattcga ccacaaaaat tgcagcagct accgcgaggg gaggaaagga aagcatatcg
     2521 taggctgatc gggtttcaat cgcaatcggc gtcgcaggtg tgggttttcg gcccgagtgg
     2581 tgggcgatgg ccccggggag caagattacc cagtgacccg ggagcgatcg agtcggggat
     2641 aaacgacaag cgactgacaa cgcctccaag tatccgttgc acgcgaaatg tggccgcaag
     2701 cacctgcccc caagcatcgc cggcgacctg aggccgacga aagaaggact cgcgctggcg
     2761 aaacgcgtgt ggaaagagat cgggtacaag ggggagtgag gaccggaaaa agccgtgggc
     2821 aaaccgcgcg aatcactgcc agcagcggag gtgtttggag ttgagcatta cctgtgtggg
     2881 gaaagggagc cgcgatgtac cagaacgtgg ctgaactcaa cagagactgt gaggattatt
     2941 ccaaatggtg gcgggcggta gaccaagagc tttaccgtta cttcgccgag cactctgacc
     3001 acgggaatct tcgttccgtg tacgccaaag ttgcgatggt cgaccgtctc tacacgactg
     3061 gcttggaacg tgcacgcaag ggaacagtcg aaatagaccg ggcgcagaag gtcgccgagt
     3121 tgcttttcgc tcgcggggag gagttggact gcggaattcg gatgatcagc ggggtgccgt
     3181 gtctgacgag agacgctctt gcggacatag cggcgttgca tggatggatt gcggaaacct
     3241 gtctcaaaga agcaggagtc ggtgcccccg tctcgttcgt ctcgaaatac ctgcacttcc
     3301 acgttcccgt ggtccccatc tacgaccagc aggcgtgcgc aagccttgcc aggtatatcc
     3361 gaggcaggtt cgggagatat gtcgctactc ctgagcgcct ccccaaactg gacgggcaga
     3421 tgcctgcgta ccggtggtat tgcgctcgtt tcgccgaatt gtgggaggag gcacaggaat
     3481 ctcgccccga aacgacagtg aaggagttgg actaccattt gtgggtgttg ggcaaaacct
     3541 aacttgcgaa ggcgcagcct gactgctttg cgtcgacacc gaaggtgaca aagttgctcc
     3601 gatatagtgt ccccggcgac gaattccagc tcgacttccg actggtggaa gctggggaac
     3661 cggtcacgtt cacgttgcgc ggggtgacct ctcgcgaccg gttcgagatc ttcccgcggt
     3721 atctggaaac ctgcgacccg gagcgcgccc gcctctcccc cgcgccgctg gcctggctcg
     3781 acgaacttgg accggttgaa ttgcccgccg gtgaggttat ccgctacacc ccggaagtgc
     3841 cgggcaacta cctcgctcga tggacaagcc ggcaatacgg tgtcgaatac cgctatttcg
     3901 ccgcggtgga ccagagctac gtgatctacc gccctgcagt ctggaactgg ccaacgccgt
     3961 tccccgcgtc gggcggggcg gacattcaca acggcggcct gcccctcgac tggtgcatca
     4021 gttcagatag caccgatgaa ccgtatgtgc cccggttgcg agacgaacaa cagcgttacg
     4081 gcgggggcgt tgtctacggc atgaacaccg cgtccgcgga cggaccgatt gatggcgcgg
     4141 ttctggcgct caggaacacc gttgcgaacc ttcgccagca cggccttgat gtcgggcggg
     4201 tggccaatct gtggtacggg ggcgggcttt cacacgacag ggttgccctg gcgcgcgccg
     4261 ccggattcga cgtgattgac gggtatgtgc cccgcgcggc cagttgcggg cttggggcgc
     4321 cgtatttccc gttctacatc ggtcccgtag attaccgttt tcccggtcaa cacggcccga
     4381 ccgacacgct cgcgttcgta ttcgactttg tcggaagctg gcatttccac ggcccgatcg
     4441 ggtttcaccg tccgaccgcc aacgggagct gggagcgggc gcggcattac atcgatctgg
     4501 cagcccgcga ggcctctctc aacgcgaaaa acagcggcgc tcacaatgtc atcacgacgc
     4561 tggtgaactt cgaaagcccg atcgcatggg gaaacccggc atatcaggtc gtctgggacg
     4621 aggggcgggg ccgggatttc ttcgacaatt acatgcacct gctggcattt gaacacacgc
     4681 ggcgatggcc catcgtgttc gcccgcgccg ccgactacgc ggattacttc cgcgcccact
     4741 acaccgggat gccgcgccgt atcgtgagtt cgatcacgca cgacatcgaa tacgaccggt
     4801 actggaccga cgaatggcac gagaagggtt tgacgcccgc gggttacgtg cccgtgaacc
     4861 agccgcttcg cgcattccgc gaagcccggg ccatgccgca gtacaacatg ccggtttccc
     4921 acgagttcat gaactacaac gacaaccggc gaacgtgccg gttcgaatac gcctgcccga
     4981 agccgctcca ctattacgac ctgacgggtg cggaaccctg gccgcaacag ccggcggaaa
     5041 ccgatatccc cgacccggat atcaccatct cgannnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnntacagcc tgctgacggg cgcctacccg tggagaaacc cccgcgcagc
     5221 aatcctcacc ggcgacgcac caatcatcat tgaaccggga tccccgaccc tgcccggcat
     5281 gctgcagcgg gcggggtacc gcactggcgt cgtcggcaaa tggcacctcg gtctcgggca
     5341 gggcgatctc gattggaacc gggacatcag cgccacgccg aacgacgtcg ggttcgacca
     5401 ctccttcatc atgccggcca ccaacgaccg cgtgccgtgc gtgtatctgg aaaaccggcg
     5461 ggtggtgaat ctggatcctg atgacccgat cgaggtgacg tatgccgaca agaaggtgtt
     5521 tcctggcgaa ctgacaggac gggaacaccc ggagcttctg agggtaaccc acagccacgg
     5581 gcacgacgga accatcgtga atggggtggg ccgcatcggg cacatgcgcg ggggcaggag
     5641 tgctctctgg accgacgaaa ccatggccga aacgttcgcg aagaaggcgg tggagttcat
     5701 cgccgcaaat cgtgaacagc agttcttcct ctactatgcc ctccaccagc ctcatgtgcc
     5761 gcgcctgccc aacccgcgct tcaaaggcgc gacgaagctc ggcgcccggg gagacgtcat
     5821 agtcgagatg gactggtgcg tcggcgaaat gctcaacgcg ctggatcggc tgggcatacg
     5881 ggagaacacc atcgtcattt tttcaagcga caacggtcct gtgctggatg acggctaccg
     5941 ggacggcgcg gaggaactgc gggacggcca tcgcccggcc ggtccgctcc ggggcgggaa
     6001 atacagcctg ttcgatggcg gcacgcgcat cccattcatt ctgagctggc cgggcagtgt
     6061 acaacccggc gaatccgccg cgctcgtcag ccatgttgac ttccacgcgt cgttcgccgc
     6121 gctgactggt cagcaactgg gccacgaaga ggcaccggac agcctcaacg tgctggatgc
     6181 tctgcttggc aggagcggca caggacgcga ggagctggtc gtggagggaa ctcgcagcaa
     6241 gacggtgtac cggcgcggca actggacata tatccctccc taccaggggc cgcaagtcaa
     6301 cgtcaacacc aatactgaac ttggcaactc gctggacccg cagctctatg atctttcgca
     6361 ggacatcggg cagatcacca acctcgcggc ggcgtttccc gcgcgggcgg cagagatgca
     6421 ggcaaggctg gactcgatcc ggcagggaac atgctcgcgc tgacccgacg gacgccattg
     6481 cggccggcgg aggcgagttc cgaaaacggt cccgatattc ccgtttccaa tttttttgtg
     6541 aacgatatat atttgacggt tgtactgggg ggtacttcac cggcctgcct cacgcgagga
     6601 tcccataacc acgcgaggtg ggaaacgtag aggagtcgcc atgtattcac cgcgtgccct
     6661 cgctttcggt ctgttgctgc tggcctttcc cctcctcgcg aacgccactc cggtcaccgg
     6721 gaacctttcc acgcagacat ggacgaaaac tagaagcccc taccgcgtca ccggcaaagt
     6781 gacggtgctt tccggtgaga cactcaccat tgagcccggc gtggatgtcc tgttcgatgc
     6841 cgatgtgcaa ttccacgtgc ggggacgtat tctggcgctc ggcgcgccgc tggacagcgt
     6901 tcgttttctg aagggctcgg cggccagatg gggcgggatg cgcatcatca aggccgattc
     6961 atgcgtgttc cattacgtcc gtttcagtga ctcgcatcgg cgcgatgcca gcgacaaatg
     7021 ggaggacatc agcggcggcg cggtgtttgt cgactcgtct gttgcggtaa ccatggcgaa
     7081 cactgtgatc tcgaggaatg ttgcggatgg gtatggcgga ggtatctgta tcgcccacac
     7141 gaaagtgacg ctgaccgatt gcaccatcgc ggacaactca gccgcggaat acggcggagg
     7201 cctttcgaac aattatgcca ccgcgtcgct gaccatgacg aactgtctcg tggtgggnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt cgaacaatta tgccaccgcg
     7381 tcgctgacca tgacgaactg tctcgtggtg ggcaatacgg ccgtcaaata cggcggcgca
     7441 atagaaaact ggggggcaaa gaccgaaatc cttggctgtg tgtttttcgg caacaccgct
     7501 accgcttcag gtgggacctc ggacggaagc atgcttgtca actggggaac cagccccacg
     7561 gtgacgatac gcaactcaat cgtctgggga aacgcccccg tcaatgccat ccgagttgat
     7621 gccggcaccg tcaccgcaac gtattcggac atccagcggt cgtccggaac gttcgccgga
     7681 accggcaata tcaatctcaa tccccagttt gtaaacaccg gcgtgcgcaa tttccgcctt
     7741 ttgccggcat cgccctgcat caacacaggt gatcccggct ctccgcttga cgcagatgga
     7801 tcgcgcgccg acatggggat aaccttctcc cccgagatca tcctctacgg tgacgtgagc
     7861 ggcgacggca ccgtgagcgc gcacgacgcc gcgctggtgg tccagcgggt ggtcaacagc
     7921 tctttggcac ttgcgcggcc gttcgccggc gacgtgacgg gaaacggctc tttgagcgcc
     7981 tatgatgcgg ctctgattct ccggaaggtc acgaacccgg gctaccgctt cccggtggaa
     8041 ggcggtacgt tgaagcctgc acttgtgtcg cgggaaccgc gacgggttca tttcgaacga
     8101 tccgggccgt gctgggtgct tgagatggac gaccctgcgg gcgtagtcgc gggtgatctc
     8161 gttctcctgc ttccagacga agcctcggca cggatcacag gcgccgtgat gtcgtccttc
     8221 cgacaggagg gaaggagcct ccacgtgtca ttcgtgcggg cggatgacgg ttcgcctgtg
     8281 ttgatctccg ttcttacgga cgcaaaaacc gctccggaga tcgtccgagc ctcgttcaac
     8341 gaaggcgcgg taaccggcgt tgcctcacaa cccgtgcagt tcgtgctgga acagaacttc
     8401 cccaatccgt tcaacccgac gaccgtggtc cggttcgcca tcccggaggc gggtgacgtt
     8461 cgcctgtcca ttcacgctgc gaacggccag cttgtacgca cgcttgtgga tcgtcccctt
     8521 ccttccggga accacgaagt ggtgtgggac gggaaggacg cgctcggtcg tgaggttgcc
     8581 agtggagtgt atgtctgccg tctccgaacg gagcgggcgg aggcaacccg gcggatgaca
     8641 cttctccgct gatccggcgc aaatcctcca gagctgtgtc acatgcggag cgcggcaggt
     8701 ctgccgcgct ccgctctttt ccggcaccgt gaacttcttc cctgttcgcg ccggaaccgc
     8761 ttttgcgtac cttttaccga tgaactcgcc gcagccgcca ggaggagaca ccatggtgag
     8821 gaatacgcga tgaatacatg cccggtactg aaacgggccc gcgcagtcgc ggcactggtc
     8881 ggccttatcg ctgtctctgg agggtgtacg ccccagacac gcgccgcgag ggtccctgag
     8941 caggatctga cgcccttcga gcagcttgtc cgacagtgga cgagggaata ccggttccgg
     9001 agaacaatcg gcgggagcga tctcgtgagc tcgcaggggg ccatcgtgga acgccccaca
     9061 cacg
//
LOCUS       DAOTNG010000111         9052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_6999_length_9052_cov_5.061354, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000111 DAOTNG010000000
VERSION     DAOTNG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6999_length_9052_cov_5.061354"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="P5179_06845"
     CDS             complement(<1..278)
                     /locus_tag="P5179_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94969.1"
                     /translation="MCNAPDRDAGRVGRIGLMGRMGPLGADTKVYVRAAYEGCAGMTV
                     VVARARSRRGDGGNEGRRGDGCENDSGGTRFVASDGSVLRGGKGTTERA"
     gene            complement(306..905)
                     /locus_tag="P5179_06850"
     CDS             complement(306..905)
                     /locus_tag="P5179_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRS94970.1"
                     /translation="MPRITTILFDLGNTLLWFDFKPVFTRLLSMSKGDPERITSAVNA
                     RYDDFAAGRMTPAEWHEFIQRICGISMPWEEFSRLWADIFWEHPEMFALARETRPRYR
                     QYLLSNTDAIHFPWCCERFPLEEVLDGYILSYELGAVKPQREIYERGLAKFGLKAEEC
                     VFIDDLEANLEGARGVGIHTVRCVSIEQVRRDLRELGVG"
     gene            complement(918..1790)
                     /locus_tag="P5179_06855"
     CDS             complement(918..1790)
                     /locus_tag="P5179_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS94971.1"
                     /translation="MTDGLPRPAPGQPVTASMTCGGLTFAGASESAHTTFVFCRQLDL
                     IFDIGSVVDAMLPIDTVLISHTHQDHLLGLTRYICLRRLQGMKPPRVLLPEASVEPVR
                     RLLAVWQELEGGADRIPPDVALEPVEAGQEVRLSGNLVARAFPVAHVLPSLGYTVITR
                     IHKLRREFYGRSGQELARLREEGVAITDPHDTPLVTFIGDTLPETLERTPDIGTSPVV
                     ILECTFLTPEHADLATPRGHLHIRDIIERIELFGNAEIILTHFSRRYRSDKVRELVQQ
                     ALPPELAPRFHVLA"
     gene            complement(1787..2887)
                     /locus_tag="P5179_06860"
     CDS             complement(1787..2887)
                     /locus_tag="P5179_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94972.1"
                     /translation="MNVFAHPMPEVYGDCADIRAITHDRTQSQKWISTTSTLFNPNDG
                     LTYVGLTHLGGDLLWAYDPATGKSRSCGYLEIRGENEVKIHRSLELGPDGRIYGATAG
                     LTDVKARLTSPGGQIWAYDPRTGVFESFGIPVPNDYVQHVTFDWKRRMAYGCTYPVPW
                     FFAYDLEARETVFSAFTGALPHRSAVDRNGRVWSGYTNTADISGENFLFFYDPDANAL
                     TWTDLHIPKCGQADKMQVDDMVALADGFVYVASVDGVLARLDPDAHEIEWLGKPARGM
                     RMCGIGEGPDGLVYCLTGAFYGMPKEDRPTRVFAFDRSTKQFRELGQIFDPEFGDGCA
                     VVHHLSIAPDGTLWVGETDNDQRSGCLWECRP"
     gene            complement(2933..3733)
                     /locus_tag="P5179_06865"
     CDS             complement(2933..3733)
                     /locus_tag="P5179_06865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRS94973.1"
                     /translation="MTHIEAASNAARAGAEVLLRHFRGLQTDDIRTKARNDFVSFVDR
                     ESETAIRGVILDAFPGHEFLGEEHGSVGGSEWRWIVDPLDGTSNYVHGVPQFAISIAL
                     QHGGTLVAGLVFDPVRGDEYVAERGAGAWRNGERLRVADGQPLEDALIGTGFPFRARH
                     VFDDYMATFREIYPRVSDVRRPGSAALDFAFVAAGATGGFWEFALSPWDIAAGAVLIE
                     EAGGVITDFEGGSAYLSSGNVVTGAPHIHRELLACVQKVLRERGSALA"
     gene            3871..6204
                     /locus_tag="P5179_06870"
     CDS             3871..6204
                     /locus_tag="P5179_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94974.1"
                     /translation="MLYLRPPIDRPLTRTEYKRGMRDMLLLGAGLMPYGTIAGVSSAI
                     FAGYVLHLGVDKAAIGLILSLVQATGLLQLVSFRLSDRVHPRALVLGLGSLEVLFGVS
                     VITVPFWVPRGWEFYAVVGLLGIAWTLANTYAPTFNAWFASIVPSDMRGRYISRRTFV
                     QYGVGIAVSLLAGKFIDLVPGMKGFRILYAVALVSGLLAFSVLYRTPLPVLGQSEEDS
                     ARGESHWRRMLAPLRHREFRSFVTFHVVWTLIAAMPGPYYTVFMIDSLKLSYSTIAIF
                     TSTQMVIMGLGFRFWGAVVDRFGGKPMVQLLFIPAAAMPALWAFATPGMYYTVPVAMF
                     FGGLFWAGLSISTSIMLYAIIPQNGQKSSYFAIWSVSVGLAASAAPAVGSAVMKALTD
                     VHFVWGGMVIDNYHTLFVLATAASVIPALLIRTLPDLGAQRPGYVISQLMRGNPFLLA
                     YNIFLLARSDSARKRANLLRALGRSHNPLAVKRIVEALSDPVPEVRRAAALALGECNQ
                     AEAVEALKKHLANPESDIKVEAARALGTLEERTAIPLLIEALWSDRLDLCVAAAEALG
                     RMDDKSARDALVSRLESGFDRTLSPAIIEALSFPVGDGPLGDLRAVRPAVAMMSSFSS
                     EVIRVQLLNAIALCLGEGDRFLTLNLEDPYERDEHLSELIIETRDALFSLPWPAEEEL
                     TVAITFERMALARSEVRVSAYIQTAARLAELLRKHFALVEPGSEPTTVEEACAALISL
                     EANGQLESLQERGMTFSVLLLHRAATQTRNEIDHRRP"
     gene            complement(6218..8695)
                     /locus_tag="P5179_06875"
     CDS             complement(6218..8695)
                     /locus_tag="P5179_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94975.1"
                     /translation="MTTFSLSENWLMASDRDIPGGSPWDPAYKPAGGWKSVTVPTTVQ
                     AELVKAGVVASPWKDWNIKGLECFERDTWWFRREFVVPETDRDSDRFELEFQGVSLCA
                     TVWLNGSPVGFLHNAHIVHRLDVTRYINRAGRNVLAIECSLAYDEMRKRIRTEISGTG
                     DNVRSYVRLCQMSFGWDFAPRLVVIGPWRPVNLICDKRAAIETIFVNTRAIAAGSADL
                     SIEVQTRFVQRMESAPKLHLSIHENEGTPPVWEHTCPIHGDGRITVEAHLPSARLWYP
                     APLGEPFRYYVKATVSCDGEVVSRKTARFGIRSVELKQNGQFTFCINGVDVFARGANW
                     VPSNSLTLDSTPERYRHLLELAHEAHFNMLRVWGGGIYEPEVFYDLCDELGIMVWQDF
                     MYACAMYPDDDTAFMENATREARQVVTRLRCHPSVVLWCGNNECQEAWTLGDWPDRAP
                     RHFGERLYDHVLPDTVRELSPGIPYWPGSPYGGPTTRSREVGDFHDWYSFPNWRDYDK
                     NAPLFSSEYGFRSVPARETVDEMISDELQWDGNSPLSRPGSLHKVWQFHHGWCGWLQS
                     ILPQFGDPTSLDQFIMLTQEAQATLMRYAVEVYRRRMFKTSGSLIWQYDDPWPAVTFS
                     LVDHFGRPKAAYFWVKEAHAPVIGMFYEKDGATGYWGISDLQNTQACSLRIRRFTHSG
                     ALLGDATFGGALLPNTATCIVENLPQNLTIASPDEEYLYAELVAGGMLSERVYHVGKR
                     CDWRLPAATIEASAARENETRVRVKLTSSGYVHFVCVSVADPAARYSTNAVDLLPGEQ
                     REIVIRTRERGPITIHSANAPTLVVEV"
BASE COUNT         1700 a         2858 c         2778 g         1716 t
ORIGIN      
        1 gcacgctccg tcgtgccctt gcccccgcga agaacggaac cgtcggacgc gacaaagcgc
       61 gtccctccag agtcgttctc gcagccatcc ccacgccggc cttcgttgcc gccatcgccg
      121 cgacggctcc ttgcgcgcgc cacaacaacc gtcattcctg cgcacccttc gtacgcggcg
      181 cgcacgtaca ccttcgtgtc cgccccaagc ggccccatcc gtcccataag acctatacgt
      241 cctacgcgcc cggcgtcccg gtcgggcgcg ttgcacaccg ggccggatgc ccctcgtccc
      301 cccccctacc ccacccccaa ctccctcaaa tccctcctga cctgctcaat cgacacgcac
      361 cgcaccgtat gaatccccac tccgcgcgca ccttcaaggt tcgcctccag atcatcaatg
      421 aacacgcact cctccgcctt gaggccgaac ttggccaggc cgcgctcgta aatctcccgc
      481 tgcggcttca ccgcgccgag ttcgtacgag agaatgtacc catcgagcac ctcttcgagc
      541 gggaaccgct cgcaacacca cgggaagtga atcgcgtcag tgttcgaaag caggtactgc
      601 cggtagcgcg gccgcgtctc ccgggcgagg gcgaacatct ccgggtgttc ccagaaaatg
      661 tccgcccaga ggcgcgagaa ctcctcccac ggcatggata tcccgcagat gcgttgtatg
      721 aactcgtgcc attctgcggg cgtcatccgc cccgccgcga agtcgtcgta acgcgcgttc
      781 accgcggacg tgatgcgctc cgggtcccct ttcgacatgg acagcaaccg ggtgaaaacc
      841 ggcttgaagt cgaaccagag cagagtgttt ccaagatcga aaagaatcgt cgtgatgcgg
      901 ggcatacggt ctccgtgcta cgcgagaaca tgaaaccggg gtgcgagttc gggcggaagc
      961 gcctgctgga cgagctcccg aactttgtcg ctgcgatacc gccggctgaa gtgggtcagg
     1021 atgatttccg cattgccgaa cagctcgatg cgttctatga tgtcgcggat gtgcaggtgc
     1081 ccccgcggcg tggcgaggtc agcgtgttcc ggcgtaagga aggtgcactc aaggatcacc
     1141 accgggctcg tgccgatatc cggcgtgcgc tccagcgtct ccggcagggt atcgccgatg
     1201 aaggtcacca gcggcgtatc gtgcggatcc gtgatggcaa ccccctcctc ccgcaaccgg
     1261 gcaagctcct gtcccgaccg gccgtagaac tcacgccgca gcttgtggat gcgcgtgatc
     1321 accgtgtacc ccagcgacgg caggacgtgg gccaccggga aggcgcgcgc cacaaggttg
     1381 ccggaaagcc tcacctcctg ccccgcctcc accggttcaa gggcgacatc gggcggaatg
     1441 cggtccgctc ccccctccag ttcctgccat acggcaagca gacgccgcac cggttccacc
     1501 gaggcttccg gaagcaacac gcgcggaggc ttcatcccct gaaggcgccg aaggcagata
     1561 taccgcgtga ggccgagcag atggtcctga tgcgtgtgcg agatcaacac cgtatcaatc
     1621 ggcagcatcg cgtccactac gctgccgatg tcgaagatca ggtcaagctg cctgcagaac
     1681 acaaacgtcg tgtgcgccga ttccgatgcg cccgcgaagg tgagcccgcc gcacgtcata
     1741 ctggcggtca caggttgccc gggagccggg cgcgggagcc cgtccgtcac gggcggcatt
     1801 cccacagaca gcccgaacgc tggtcgttgt ccgtctcccc cacccagagc gtgccgtccg
     1861 gcgcgatgga aaggtggtga acgaccgcgc atccgtcacc gaactcgggg tcgaaaatct
     1921 gccccagttc gcggaactgc ttcgttgaac ggtcaaacgc gaacacacgc gtcggccggt
     1981 cctctttggg catgccgtag aacgcgccgg taaggcagta cacaagcccg tccggccctt
     2041 cgcctatgcc gcacatgcgc atgccccggg ccggtttgcc gagccattca atttcgtgcg
     2101 cgtccgggtc aagccgggcc agcacgccat ccacgctggc cacgtagaca aacccgtccg
     2161 caagcgcaac catgtcatcc acctgcatct tgtccgcctg tccgcacttc ggaatgtgca
     2221 ggtccgtcca ggtgagcgcg ttcgcgtcgg ggtcgtagaa aaagaggaag ttctcgccgg
     2281 atatatccgc cgtattggtg tacccagacc acacccgtcc gttgcggtcc accgcggacc
     2341 ggtgcggcag cgccccggtg aacgcggaaa acaccgtttc tcgcgcctcg agatcatacg
     2401 cgaaaaacca ggggacgggg tacgtgcagc catacgccat gcggcgcttc cagtcgaacg
     2461 tcacgtgctg gacatagtca ttcggaaccg gtatcccgaa cgactcgaaa acgccggtgc
     2521 ggggatcata cgcccatatc tgcccgccgg gcgacgtgag acgggctttc acatcggtaa
     2581 ggcctgcggt cgctccataa atccgcccgt ccgggccaag ctcaagagag cggtgaatct
     2641 ttacctcatt ctctccccgg atctccagat acccgcagga acgcgatttc cccgtggccg
     2701 ggtcgtacgc ccagagcaga tccccgccga gatgggtaag cccgacgtac gtcaggccgt
     2761 cgttcgggtt gaacagcgtg ctcgtcgtgg aaatccactt ctgcgattgc gtcctgtcgt
     2821 gcgtgatggc gcgtatatcg gcgcagtcgc cgtacacctc cggcatcggg tgcgcgaaca
     2881 cgttcattgc ctgttctcct ttgtcggtcc tgattcggtt cggcgcccgg cactatgcga
     2941 gcgcactgcc tcgctccctc aacaccttct gcacgcaggc gagcaattcc cggtggatat
     3001 gtggcgcgcc cgtcaccacg ttccccgatg aaagatacgc gctgcctccc tcgaaatccg
     3061 tgatcacgcc gcccgcttct tcgatgagga ccgcccccgc cgcaatgtcc cagggcgaaa
     3121 gtgcaaactc ccagaaacca cccgtggcac cggccgccac gaacgcgaaa tcgagggcgg
     3181 cggaacccgg ccgtctgacg tcggaaacgc ggggatagat ttcccggaat gtagccatgt
     3241 aatcgtcaaa cacgtggcgg gctcggaacg gaaacccggt gccgatcaac gcgtcttcca
     3301 gcggctgccc gtccgccacg cgaaggcgtt ctccatttcg ccacgcgcca gcaccccgct
     3361 cggcaacata ttcatcgccg cgaaccgggt cgaacacgag gcccgcaaca agagttccgc
     3421 cgtgctgcag ggcgatggaa atggcaaatt gaggaacgcc gtgcacgtaa ttgctggtcc
     3481 cgtcgagcgg atcgacaatc caccgccact cgctgcctcc cacggaaccg tgctcttccc
     3541 cgaggaattc gtggccggga aacgcgtcga gaatcacgcc cctgatggcc gtttccgact
     3601 cccggtccac gaacgaaacg aagtcatttc gcgcctttgt gcggatgtca tcggtctgaa
     3661 gcccgcggaa atgcctgaga agcacctcgg ctcctgcccg ggccgcgttg gatgctgctt
     3721 cgatgtgtgt cagactgacc cctgtcccgg agatggcctc cgttatcgct ggtgtggtgc
     3781 attccttcca aaagcccgta catgggctct atatttagaa ggcaaatcgt cgtcctcgtg
     3841 tcaatacctc cacgaaaaaa aagaagagct ttgctttacc ttcggcctcc tatcgaccgg
     3901 ccgcttacgc gcaccgagta caagcgcggc atgcgcgata tgctgctcct cggcgccggc
     3961 ctgatgccct acggtaccat cgcgggcgtc tcgagcgcca tttttgccgg atacgtgctc
     4021 cacctcggcg tcgacaaagc cgcaatagga ctcatccttt ccctcgtcca ggcgaccggg
     4081 ctcctccagc tcgtaagttt ccgcctcagc gaccgagttc acccgcgcgc actcgtgctc
     4141 ggcctcggtt cgctggaggt ccttttcggc gtgtccgtga tcaccgtccc cttctgggtc
     4201 cctcgggggt gggagttcta cgcggtggtg ggcctcctcg gcatcgcgtg gactctggca
     4261 aacacgtacg cacccacctt caatgcctgg tttgccagta ttgtgccgtc agatatgcgc
     4321 ggtcgctaca tcagccggcg cacgttcgtc cagtacggcg taggaatcgc cgtctccctg
     4381 ctggcgggga agttcatcga cctcgttccc gggatgaagg gcttccgaat cctgtacgcc
     4441 gtggcgcttg tttccgggtt gcttgcgttc tccgtgctgt atcgcacacc gttgcccgtc
     4501 ctgggccagt ctgaggagga ttccgcaaga ggcgagtcgc actggcggcg tatgctcgcc
     4561 cccctgcgcc acagagaatt ccgctccttc gtcaccttcc acgtagtctg gacactcatc
     4621 gccgccatgc cgggacccta ttacacggtc ttcatgatcg acagcctgaa gctttcgtac
     4681 tccacaatcg ccatcttcac ctccacgcag atggtgatca tggggctcgg atttcgcttc
     4741 tggggagcag tggtggatag attcggcgga aagccgatgg tccagctcct gttcattccg
     4801 gccgctgcaa tgccggctct atgggcattt gccacccccg ggatgtacta caccgttcct
     4861 gtggcgatgt tcttcggcgg gcttttctgg gcgggtcttt ccatctccac gtccatcatg
     4921 ttgtacgcga tcatacctca gaacgggcaa aagagctcct acttcgcgat ctggtccgtg
     4981 tccgtagggt tggcggcttc cgcggcgccg gcggtcggca gcgcggtaat gaaggcgctg
     5041 acagacgtgc atttcgtatg gggcggcatg gtaattgata attaccatac gctcttcgtt
     5101 ctggcgactg ccgcgagtgt gatcccagcg ctcctcatca ggaccctgcc cgacctgggc
     5161 gcacaacggc cgggatacgt aatcagtcag ttgatgcgcg ggaacccgtt tcttcttgcg
     5221 tacaacatct ttctccttgc acggtcggat tccgcgcgga agcgggcgaa tctgctgcgg
     5281 gcactgggcc gctcccacaa cccgctggcg gtgaaacgca ttgtggaggc gctgagtgac
     5341 ccggtgccgg aagtccgcag ggcagcggca cttgcgctgg gcgaatgcaa ccaggcggag
     5401 gcggtcgaag cgctgaagaa gcaccttgcc aacccggaat ccgacattaa ggtggaagcc
     5461 gcgcgggcgc tgggaacgct ggaagagcga acggctatcc ctctgcttat cgaggcgctc
     5521 tggtcggacc ggctggacct gtgcgttgcg gcggcggaag cgctgggcag gatggacgac
     5581 aaaagcgccc gggatgcgct cgtgtcgaga ctggagtccg gttttgaccg caccctgtcg
     5641 cccgcgatta ttgaagccct gagtttcccg gtgggcgatg ggccgctggg ggatcttcgg
     5701 gcggtgcggc cggcggttgc gatgatgagt tcgttttcgt ccgaggtgat acgggttcag
     5761 cttctgaacg cgatcgcgct gtgcctcgga gaaggcgacc ggttcctcac gttgaatctg
     5821 gaggatccgt acgaacggga tgagcacctt tcagagctca tcatcgaaac gcgcgacgcg
     5881 cttttcagtc ttccctggcc ggccgaggag gaattgaccg tcgcgatcac cttcgagagg
     5941 atggcactgg cgcggtcgga ggtgcgggtt tccgcctaca tccagaccgc ggcgcgtctg
     6001 gcggaacttc tccggaagca ttttgctctt gtcgaacccg gcagcgaacc gacgaccgtg
     6061 gaggaggcgt gcgccgcgct gatttccctc gaggcgaacg ggcagcttga atcgttgcag
     6121 gagcggggaa tgacgttttc cgttctgctc ttgcaccgcg cggcgacgca gacgcgcaac
     6181 gaaattgacc acagaaggcc ctgataccgg gccccggcta tacctcaacg acgagggtgg
     6241 gggcgttggc gctgtgaatg gtgattgggc cgcgctcccg cgtccggatc acgatttccc
     6301 gctgctctcc cggcagaaga tccaccgcat tcgtggaata acgggccgcg gggtcggcga
     6361 cactgacgca gacgaagtgc acgtaccccg aagaggtgag tttcacccga accctcgttt
     6421 cgttctctcg cgccgcactt gcttcgatgg tggccgcggg gagccgccaa tcacaccgct
     6481 ttcccacgtg atagacgcgt tctgagagca ttcctcccgc gacgagttcg gcgtagagat
     6541 actcctcgtc cggcgacgct atcgtaagat tctgcggcag gttctcgacg atgcaggtcg
     6601 ccgtgttcgg caggagtgct cctccgaatg tagcgtctcc caggagcgcc ccggaatggg
     6661 tgaagcgcct tatgcggagg ctgcatgcct gcgtattctg cagatcgctg attccccagt
     6721 atcccgttgc gccgtctttc tcgtagaaca tgccgatgac cggtgcgtgg gcttctttca
     6781 cccagaaata ggcggctttg ggtcgtccga aatgatcaac gaggctgaag gtgacggcgg
     6841 gccaggggtc gtcgtattgc cagatgagcg atccgctggt tttgaacatg cgtcgacggt
     6901 acacctcgac cgcgtagcgc atgagggttg cctgagcttc ctgggtaagc atgatgaact
     6961 gatcgaggct ggttggatcg ccgaactgcg gcagaatgga ctgcagccac ccacaccagc
     7021 cgtgatggaa ctgccagact ttgtgaagtg atccggggcg gctcagcggg ctgttgccat
     7081 cccactggag ttcgtcgctt atcatctcgt ccactgtttc gcgagcggga acagaacgga
     7141 atccgtattc gctgctgaac agcggcgcgt ttttgtcgta atccctccag ttggggaagc
     7201 tgtaccaatc gtggaagtcg cccacttcgc gggaacgcgt ggtcgggccg ccgtacgggc
     7261 tgccgggcca gtacggaatg ccgggggaca gttcgcgcac tgtatccggc aacacatggt
     7321 cgtacagccg ttcgccgaaa tggcgtgggg ctctatcagg ccaatcgccg agcgtccacg
     7381 cttcctggca ttcgttgttc ccgcaccaga ggaccaccga cgggtggcac cgtaatcggg
     7441 ttacgacctg tctcgcttcg cgcgtggcgt tctccatgaa cgccgtgtcg tcgtcagggt
     7501 acatagcgca ggcgtacatg aaatcctgcc acaccatgat gccgagttcg tcacagaggt
     7561 cgtagaacac ttccggttcg tagatgcctc caccccacac gcgcagcatg ttgaaatgcg
     7621 cctcgtgagc cagttcgagc aggtggcggt agcgttcggg agtggagtcg agcgtaaggc
     7681 tgttggaggg aacccagttg gcgccgcggg cgaacacgtc aaccccgttg atgcagaagg
     7741 tgaattgtcc gttctgcttc aattccaccg atcgtatccc gaagcgtgcc gttttccgcg
     7801 agacaacttc cccgtcgcac gacacagtgg ctttgacgta gtaccggaag ggctctccca
     7861 acggagcggg gtaccacagc ctcgccgacg gaaggtgcgc ctccacggtg atcctgccgt
     7921 cgccatgaat ggggcaggtg tgctcccaga cggggggtgt gccttcgttc tcgtggattg
     7981 aaagatggag tttcggcgct gattccatcc gctggacgaa ccgggtctgc acctcgattg
     8041 agaggtcggc actgccggct gcgatggccc gggtgttcac gaagatggtt tcgatggcgg
     8101 cacgtttgtc gcagatgagg ttgaccggcc tccatggtcc tattaccacc agccgcggcg
     8161 caaaatccca cccgaagctc atctggcaca gacggacata ggaacggaca ttgtcgcccg
     8221 ttccgctgat ctcggtccgg attcgtttgc gcatctcgtc gtatgcgagg gaacactcga
     8281 ttgcgagcac gttgcgaccg gcgcggttga tgtaccgggt gacgtcaagc cggtgcacga
     8341 tgtgggcatt gtgaaggaag ccgacggggg agccgttcag ccagacggtg gcacacaggc
     8401 tgacgccctg gaactcgagc tcgaaacgat cgctgtcacg atctgtctcc ggaacgacaa
     8461 actcgcgtcg gaaccaccat gtatcccgct cgaagcattc gagacccttg atgttccaat
     8521 ctttccacgg cgacgcgact accccggctt tgacgagctc ggcctgtacc gtggtcggca
     8581 cggtgacgga cttccagccg cccgcgggtt tgtacgccgg atcccacggc gacccgccgg
     8641 ggatgtcgcg gtcggacgcc atgagccagt tttcggaaag ggagaaggtt gtcattggca
     8701 gggccctcgt ggaggaaacg tgcgcgaacg attagcaagg tagcgaacag ggcggcatga
     8761 gggcaaacgg gtaggcgggg gaggagagga aggggctcgc acgaagacac gaagacgcga
     8821 aggtaaaaag ggcatcacac gaagacacga agagcacgaa gaagacaaag gggctcgcac
     8881 gaaggcacga aggggaaatg agaaaagggg cgcacacgaa gacacgaagg acacgaaggg
     8941 atgatgaaag aaaagagtct cacacgaaga cgcgaagacg cgaagaagga ggacaggggg
     9001 attcgtgtgg gatcgatggg cgaagaatga atggcggttc ggtgcggcac gg
//
LOCUS       DAOTNG010000112         9024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7021_length_9024_cov_5.497714, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000112 DAOTNG010000000
VERSION     DAOTNG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7021_length_9024_cov_5.497714"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5179_06880"
     CDS             <1..469
                     /locus_tag="P5179_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94976.1"
                     /translation="ELGEAPVDLALAVMVLRGTPEGTAKVAELCEGRALVDMLDVDSL
                     FMPEQAAVTEDESHEEAQAFLDDLGIDQDAVEAGGAELETGGVRFVLTEEEKRAYISR
                     AVGRHTHLIEPIMKATEAALTWEEVDRAINDLVPDDDRDEDLSARFRERLGRR"
     gene            566..1864
                     /locus_tag="P5179_06885"
     CDS             566..1864
                     /locus_tag="P5179_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRS94977.1"
                     /translation="MHFLEKLIESRQAQVGIVGLGYVGLPLAVEFAGAGLRVIGFDMQ
                     PAKVDSVNNGVSYIGDVPDERLVPLTKSGRLRATNNLSDLGQCDAICICVPTPLDKNK
                     TPDISYVASTADAIAQNLRDGQLIVLESTTYPGTTEEIILPRLTATGRIVGKDFYLAF
                     SPERIDPGRKDFTTANTPKVVGGVTPQCTELAAALYSTFNPRVHRVSSPRVAEMEKLL
                     ENIFRLVNVSMINEMALLADRLGVDIWEVIEAASTKPYGFMPFYPGPGLGGHCIPIDP
                     FYLSWKAKEVNFSPRFIELAGEINDMMPHYVTTKVIVALNRQKKCVNGSKILVLGVSY
                     KKDVEDVREAPAVRIIRELWRKFALVTYCDPHVRSLSIDGKPVERVELTDEALREADC
                     VLILTDHTAFDFDRVAEKSRLIVDTRNAITRRDLSHVYHL"
     gene            1884..2417
                     /locus_tag="P5179_06890"
     CDS             1884..2417
                     /locus_tag="P5179_06890"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRS94978.1"
                     /translation="MAKQDIQFTAYLSQSLEVLSRGGALLVSTGKNGRPNVMTIGWGT
                     VGWIWGKPIFCVLVRPSRYTHECLEATPEFTVNIPPRSLAGAVAYCGKVSGRDEDKFA
                     AQNLTATPGRRVAPPIVEECVIHYECRVVEKNNVIQETLSAEIDRSAYRSGDYHTLYF
                     GEIVAVYADADALAKLA"
     gene            complement(2509..3840)
                     /locus_tag="P5179_06895"
     CDS             complement(2509..3840)
                     /locus_tag="P5179_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94979.1"
                     /translation="MAEMLFTFASYATRIEGSLTPLKGMKRLADTLHPYGVKITWLVN
                     HDSAEALKSDLTEWHDRFGDDIAVMVPHFKGGAPELVAQARAARQRMQKILPWTRADI
                     AGNHVRSRESLESLEAAGFIGLWGFCWEQIEVDDITDRGCPWGFYYLDPAFRTAPNTR
                     PKGLIGMEWTARDLLKAYHSGNPCIYSTDVNDVARAGMCLWRDIEYLKGMFDNYYRNL
                     RYNDLVLYQVHQEAHEMMPEFRCYSEEDCREAEEMLDELADYVVGHAHIRTATLSEAA
                     DVYRERNEFTEPSYMLWEDTPAPPYNLDYSRGTPRGPWPKTFLFYDSECQMMFVDGKF
                     EPVCLRNYADRETTARRYFEEVDIPRVRLRSHTRSPYGMELHLTVEAPKAMPFGITIW
                     EDFGPYLLAESVSEPTGTDLMASRILSQELLFLRYNVPEGKTELRVRFNRK"
     gene            complement(3879..4844)
                     /locus_tag="P5179_06900"
     CDS             complement(3879..4844)
                     /locus_tag="P5179_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS94980.1"
                     /translation="MATKGLQVGFIGAGGMAQSHMKGVARLSDVKIAAICDVDEQRAQ
                     SVAKEYHAAAYTDFRAMLRKKLDAVYIVLPPFAHGEVEKAVIKKGLPFLVEKPIALSA
                     KLGEKILDAVEKKNLITCVGYQLRYMSSIQQAKAFLDKKTLGMIVGYYWSEMIRGAWW
                     PYMEKSGGQVVEQATHIVDLMRDLGGEIVEVDARMEQRVHGKNDGITIPDTYSVRFGF
                     ANGAIGTLTTCCLVGEWNIGLDIMVDRARLHWKIDELTVAPDSVTLPAVESFKAGDID
                     ATFLNAVKTGDRSAIRSDYRDALKTLKVTLAATESASKKKTIVLK"
     gene            complement(4919..5869)
                     /locus_tag="P5179_06905"
     CDS             complement(4919..5869)
                     /locus_tag="P5179_06905"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS94981.1"
                     /translation="MNHRFRFLTVLAAAGCVLAPFAQAARAIEVGSTGYKFLQMNPDA
                     SAMALGRTFSTERGGATALAGNPAGLAGVGTVSAVVSHTEWLSTIRHEFAGAAIRRFG
                     GVIAASFRTQSSGDIPLRTADTGGGLIGLPTPAPLGTYGVYDAALTIGYARATTLADV
                     GGSVTYAYEKIFTAESSAMCASVGMQRRAGDLILGAAARNFGFSSRMKYERTPLPWDI
                     QAGAAYDRHIRDVPFRLVGDFRYAPDWYATFHLGTQATFGNVLVARAGYENGLTRNLD
                     TAGFSGGLGFLCRGFRVDYAYQPQRTGLGSRHVFAVAIGG"
     gene            complement(5866..6798)
                     /locus_tag="P5179_06910"
     CDS             complement(5866..6798)
                     /locus_tag="P5179_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94982.1"
                     /translation="MKTARFLLAFAVLAFCSGTARASGGYANPLYRYGIGSNVLGVGG
                     AVVSLAYDPASILVNPAGAAFAGRGRIAASHSALAFDRTINFIGFSRQIDPQAGFSVS
                     WVNSGVSDLIGYDLSGNSTGPLDNSSNTVAATFASKFGPIAAGVSAKWHRVSLNKQQS
                     AGWSFNVGALARPLPGFSIGVCARDLLGTTVWTTETDVGQLRSEDTFPVTLSGGVSYA
                     VARLGTTLAADFEHVGSEGDYLHVGTSVVVNQYLRVRAGYRWIGIAESCRKAAFAGGI
                     GLSTAFGDNRVHFDYSVLNEALGAAHSLGMAFEL"
     gene            complement(6795..8363)
                     /locus_tag="P5179_06915"
     CDS             complement(6795..8363)
                     /locus_tag="P5179_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS94983.1"
                     /translation="MPRPATVLGARIGLESNEAAFFARADTTLWVSARRTLSRLAGSG
                     VSQFDWVTYDVGTITSRPDEAITAYAVSGDRIAAATSHVVNSSSGSYYVGDGLYLSRD
                     GGASWFPIPMTALFADRTNMAVPGGDVQSFGVWFDGTDLWAAFTTEFAVMTPDFGVTW
                     HRYRPDSTNNPQPNPFTGDPGRLHRYRHLNYRAFDGLAVHDTLWVSTNAGINRSTDRG
                     VTWQNFDAARAGISGDFVPSVAVDTVNGILWASTQSTGLDENYLKNNPQDVFTDGVFD
                     SLDYDLDRDGILDGPGKNGVSWTRDGGQTWHTYVPVDDATVGVDYRAWGFAFNGQKVL
                     VGGSIGSFDALLRSDDLGATWRLQPIRTALGDTSRSGQGVTDVAWQNGTIWVTTGLGL
                     ARSTDDGQTWAYILRYPQTELLGGSGVAYPGGDAAGIATYAFPSPCAPLRGTPARIVF
                     ALAATADVTIEVYDAGGARVRTLRQTSLPPGNQTVEWDGRSDGGRVVANGVYLYVITT
                     STGHTARGKMMVFN"
     gene            8693..>9024
                     /locus_tag="P5179_06920"
     CDS             8693..>9024
                     /locus_tag="P5179_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94984.1"
                     /translation="MKHSVLVFVLLLVSAISVAGQAIEEDAFLLPDRVRSNPDKFTFA
                     ILGDRTGSDRQSWRIFDRSVEEINLSRPDFVIMVGDMIEGHDYPEEVAAQWEEFQSHA
                     DRLEAPFM"
BASE COUNT         1867 a         2809 c         2594 g         1754 t
ORIGIN      
        1 cgaactcggt gaagctccgg tggacctggc acttgcggtg atggttctgc ggggaacgcc
       61 ggaaggaacg gccaaagttg ccgaactgtg cgagggccgc gctcttgtcg acatgctcga
      121 cgtggactcg ctgttcatgc cggagcaagc cgccgtcacc gaggacgaat cgcacgaaga
      181 agcgcaggcg tttctcgatg atctgggaat tgaccaggat gctgtggaag ctgggggagc
      241 ggagctcgaa accggcggcg ttcgtttcgt cctcacggaa gaggaaaaaa gagcgtacat
      301 cagtcgcgcg gtggggcggc atacgcatct catcgagccg atcatgaagg ccaccgaagc
      361 cgccctgaca tgggaagaag tggaccgtgc catcaacgac ctcgttccgg atgacgaccg
      421 cgatgaagac ctgagcgcac ggttcaggga acggctgggt cgtcgctgag agggatctgg
      481 tggctttgcg tgagcacatt gcgggtgggc tggcttcccg ggtggggcgc cagccctttt
      541 tcgcgtcgaa aggcgaacaa aaagcatgca ttttctggaa aaactgatcg aatcgcgaca
      601 ggctcaagtg gggatcgtcg ggctcgggta cgtcggcttg ccactggctg ttgagttcgc
      661 gggggcgggc ctccgcgtga tcgggttcga tatgcagccc gccaaggtgg attccgtcaa
      721 caacggtgta tcctacatcg gcgacgtgcc ggacgaacgt ctcgtaccgc ttacaaagag
      781 cgggcgcctc cgcgcgacca acaacctgtc tgatctgggt cagtgcgacg cgatatgcat
      841 ctgcgttcct acgccgctgg acaaaaacaa gacacctgat atctcgtacg tcgccagcac
      901 cgccgacgca atcgcgcaaa acctgcgaga cggtcagctt atcgttctcg agagcactac
      961 ctatccggga acgacggagg aaatcattct tcctcgtctg acagcgaccg gccgcatcgt
     1021 gggaaaggac ttctatctcg ccttctcccc ggaacggatt gaccccggcc ggaaggattt
     1081 cacaaccgcc aacacgccga aagtggtcgg aggcgtcacc ccgcagtgta ccgagcttgc
     1141 cgcggcgctt tacagcacct tcaatccccg ggtgcacagg gtcagctcgc cgcgtgtcgc
     1201 ggagatggaa aagttgctgg agaacatctt ccgcctcgtc aacgtgtcaa tgatcaacga
     1261 aatggcgctc cttgccgaca ggcttggagt ggacatctgg gaagtcatcg aagccgcaag
     1321 caccaagcct tacgggttca tgccgtttta cccgggaccc ggtctgggag gccactgcat
     1381 accaattgac ccgttctatc tttcgtggaa agcgaaagag gtgaatttct cgccgcgctt
     1441 catcgaactc gccggcgaaa tcaatgacat gatgccccac tatgtcacca caaaagtgat
     1501 cgtggcgctg aaccggcaga agaaatgcgt gaacgggtcc aaaatcctcg ttctcggtgt
     1561 ttcctacaaa aaggacgtgg aagacgttcg cgaagctccc gcagtgcgca ttatccggga
     1621 gctctggagg aagttcgctc ttgtcacgta ctgcgatccc cacgtccgct cactttccat
     1681 cgacggaaaa cccgtcgaac gcgttgaatt gaccgacgag gctctccggg aagcggactg
     1741 cgtactcatt ctcaccgacc acactgcgtt tgattttgat cgcgtcgccg aaaagagcag
     1801 gctgattgtg gatacgcgca acgccatcac gcgccgcgac ctctcccatg tgtatcacct
     1861 gtaggtgcac ggaggacaga aagatggcca aacaggatat ccagttcacg gcgtacctgt
     1921 cgcaaagcct cgaagtgctt tcacgtggag gtgcccttct cgtctccacc ggaaaaaacg
     1981 ggcgcccgaa cgttatgact atcgggtggg gaacggtcgg gtggatctgg gggaagccca
     2041 ttttttgcgt gctcgtccgc ccgtcacgtt acacgcacga atgcctcgag gcgacccccg
     2101 agttcacggt gaacattccg ccgcgaagcc ttgccggtgc ggttgcatac tgcggaaaag
     2161 tctccggacg tgacgaagac aaattcgccg cacagaacct gacagcaaca ccgggtcgcc
     2221 gcgttgcccc accgattgtc gaagaatgcg tgatccatta cgagtgccgc gtagtagaga
     2281 aaaacaacgt gattcaggag acgctttcgg ctgaaattga ccggtcagcc taccgcagtg
     2341 gcgattatca caccttgtat ttcggggaaa tcgtggctgt gtatgcggac gcggatgccc
     2401 tggcgaaact ggcgtgaaga actgacggcg cggttgatcg gttgtgtggg caaaaatgga
     2461 aggggcgcgg ctgcaccgcg ccccttcgtc attggaacag gaatgcgtct acttccggtt
     2521 gaacctcacc cggagttccg tcttgccctc aggcacgttg taccgcagga aaagcagctc
     2581 ctgtgaaagg attctggacg ccatgagatc cgttcccgtc ggctcagaga cagattccgc
     2641 gagcagatag ggtccgaagt cctcccagat cgtgatgccg aagggcatgg ctttcggcgc
     2701 ctcgacggtg aggtgcagct ccattccgta aggtgatcgc gtgtgcgacc ggagccgcac
     2761 ccgcgggata tccacctcct caaagtatcg cctggcagtc gtttccctgt cggcgtagtt
     2821 cctgaggcac accggttcga atttgccgtc cacgaacatc atctggcatt ccgaatcgta
     2881 gaacaggaac gtctttggcc acgggccccg cggagtacct cgcgagtaat caaggttgta
     2941 cggaggagcc ggcgtgtctt cccagagcat gtaactcggc tctgtgaact cgttccgctc
     3001 ccggtacaca tcagccgctt cggaaagagt ggccgtcctg atgtgtgcat gtccgacaac
     3061 gtaatccgcg agttcgtcca gcatctcttc tgcttcccgg caatcctctt ccgagtaaca
     3121 gcggaattcc ggcatcatct cgtgggcttc ctgatgaacc tggtacagga cgagatcgtt
     3181 gtaccgcagg ttccgatagt agttgtcgaa catgcccttg aggtattcga tatcccgcca
     3241 gaggcacatt ccggcgcgcg cgacatcgtt tacatccgtg ctgtagatgc acggattacc
     3301 tgaatggtac gccttgagca aatcacgcgc ggtccattcc atgccgatca gccctttcgg
     3361 gcgtgtgttc ggagcggtcc ggaacgccgg atcgaggtag taaaagcccc aggggcagcc
     3421 acggtccgtg atgtcatcca cctcgatctg ttcccagcag aatccccaca acccgatgaa
     3481 cccggctgct tcgagcgatt ccagcgattc cctgcttcgc acgtggttcc ccgcgatatc
     3541 ggcgcgtgtc cacgggagga tcttctgcat cctctgtcgg gccgcccgcg cctgggccac
     3601 aagttccggt gccccgccct tgaaatgggg gaccatcacc gcaatgtcat cgccaaaccg
     3661 atcgtgccat tcggtcaagt cgcttttcaa cgcctcggcc gaatcgtggt ttacaagcca
     3721 ggtgatcttc acgccatacg gatgaagcgt gtccgcgagc cgcttcatac ctttcagcgg
     3781 cgtaagactc ccctcaatcc gcgtggcgta cgaggcgaac gtgaacagca tctcggccat
     3841 gtgaaatgtg cctttcgtaa tgcagagtgc gtggaacgtt atttcaacac gatcgttttc
     3901 tttttgcttg cagattccgt cgccgcgagc gtgactttca gcgtcttcaa cgcatcacga
     3961 tagtcggagc ggatagccga gcgatccccg gttttcacgg cgttgaggaa cgtcgcgtca
     4021 atgtcaccgg ctttgaagga ctcaacggca ggaagcgtga cggaatccgg cgcaaccgtg
     4081 agttcatcaa tcttccagtg caatcgggcg cggtccacca tgatatcaag cccgatgttc
     4141 cattcgccga ccaggcaaca ggtggtcagc gtgccaatcg ccccgtttgc gaatccaaat
     4201 cggacgctgt acgtgtccgg aatcgtgatg ccatcgttct tgccgtgcac gcgttgctcc
     4261 atgcgggcgt ctacctcaac aatttctccg ccgaggtcgc gcatgaggtc aacgatgtgg
     4321 gtcgcctgct ccacgacctg ccctcccgat ttctccatgt agggccacca cgcgccacga
     4381 atcatttcgc tccaatagta cccgacgatc atcccgagcg ttttcttgtc aaggaacgcc
     4441 ttggcctgct ggatgctcga catgtaccga agctgatacc cgacacaggt aatcaggttc
     4501 ttcttttcca ctgcgtcgag gatcttctcg ccgagtttcg cggacagcgc aatcggtttc
     4561 tccacaagga acggaaggcc cttcttgatc acggcctttt ccacttctcc gtgggcgaac
     4621 ggcggcagca cgatatacac ggcgtcgagt ttcttgcgca gcatcgcccg gaaatccgtg
     4681 tacgccgccg cgtggtattc cttggccacc gattgtgcac gctgctcgtc tacatcgcat
     4741 atcgcggcga tcttcacatc actgagcctg gcgacgcctt tcatatggct ctgcgccatt
     4801 ccgcccgcgc cgatgaaccc tacctgaagc ccctttgttg ccatgcgatg atctccttag
     4861 aatctgggta tgaaacctgg atctgtcccg gaagccacaa gctccgttcg tcttcgagtt
     4921 aaccgccgat cgctactgca aacacgtgtc gactgccgag cccggttcgt tgcggctgat
     4981 acgcgtaatc cacacgaaat ccgcgacaca ggaagccgag tccaccggaa aatcccgcag
     5041 tatcgagatt ccgcgtcaga ccgttctcgt accccgcccg ggcaacaagt acgtttccga
     5101 aggttgcctg cgttcccaga tgaaaggtcg cgtaccagtc cggcgcgtag cggaaatccc
     5161 caaccaggcg aaacgggaca tcgcggatgt gtcggtcgta cgccgcgcct gcctggatat
     5221 cccacggaag cggagttctc tcgtatttca ttcttgatga gaaaccgaaa ttgcgggcag
     5281 ccgcgccaag gatcaggtcc ccggcgcgcc gctgcatacc aacgctcgcg cacatggcgg
     5341 aagactccgc cgtgaagatc ttttcatacg cgtaagtgac acttccgcct acatctgcca
     5401 gagtcgtcgc gcgggcgtag ccgatcgtca gcgcggcgtc atacacgccg tacgtgccaa
     5461 gcggtgcggg ggtcggaagg ccgatcaaac caccgcccgt gtccgcagtg cgaagcggga
     5521 tgtctcccga ggactgagtc cggaaggagg cggcgatgac accgccaaat cgccgaatgg
     5581 ccgcgccggc gaactcgtga cgaattgtgg acagccattc tgtgtgggat acgacagcgg
     5641 ataccgttcc aacaccggcc agtcccgccg ggttgcccgc aagcgcagtc gccccgccgc
     5701 gttcggtgga gaaggtcctt cccagagcca ttgccgacgc atccgggttc atctgcagga
     5761 atttgtagcc ggtgctgccg acttcgatcg cccgagcggc ttgtgcaaat ggcgcgagca
     5821 cacaccctgc cgccgccagc accgtgagga aacgaaatcg atggttcaaa gctcgaatgc
     5881 cattcccaga gaatgcgccg ccccgagcgc ttcgttgagc accgagtaat caaaatgcac
     5941 tctgttatcc ccgaacgcgg tagataaccc tatcccaccg gcgaaagcgg cctttctgca
     6001 ggattcggcg atgccgatcc accggtaccc cgcccgcacc cgcagatact gattgacgac
     6061 gacggaggtg ccaacgtgaa gataatcgcc ttcgcttccc acatgctcga aatctgccgc
     6121 cagcgtggtg cccagccgtg caacggcgta cgacacgccc cctgacagcg taaccgggaa
     6181 cgtatcctcg ctcctgagct gtcccacgtc cgtttccgtc gtccacaccg tggtgccgag
     6241 aagatcgcgg gcgcaaaccc cgatggagaa gccgggaagc ggccgggcga gagcgcccac
     6301 gttgaacgac catcccgccg attgctgctt gttgagactc acccgatgcc atttcgcaga
     6361 aacacctgcg gcaatggggc cgaatttgct cgcaaaggtg gccgcgaccg tattgctgct
     6421 gttatcaagc ggacccgtgg agttgccgct caggtcatac ccgataagat cgctcacccc
     6481 gctgttcacc cagctcacgc tgaatccggc ctgcgggtcg atctgccgcg aaaaaccgat
     6541 gaaattgatt gtccgatcaa aggccagcgc ggaatgggat gccgcgattc ggcctctccc
     6601 cgcaaacgcg gctcccgctg gatttacgag aatgctggcc gggtcatacg caaggctgac
     6661 cacggcgccg cccaccccca gcacgttgga accaatgcca taccggtaga gcgggttggc
     6721 gtacccgccc gatgcgcgcg ccgttccact gcaaaaggcc agcacggcga aggcaagcag
     6781 gaatcgggcc gtcttcaatt gaacaccatc atctttcccc gtgcggtgtg gccggttgag
     6841 gtggtgatca cgtagaggta gacaccgttc gccaccacgc gtcccccgtc agaacggcca
     6901 tcccattcga ccgtctgatt gccgggtgga agcgatgtct ggcgcaacgt gcgaacccgc
     6961 gcgcctcccg cgtcgtacac ctcgatcgtg acgtccgccg tggcggcaag cgcaaacacg
     7021 atccgcgccg gtgtccctcg aaggggtgcg cacggggacg ggaacgcgta ggttgcaatt
     7081 ccggcggcat caccccccgg ataggctacg cctgagcccc cgagcagttc cgtctgtgga
     7141 tagcgaagga tgtacgccca ggtctgaccg tcatcagtgg aacgtgcaag accgagcccc
     7201 gtggtgaccc agatcgttcc gttctgccag gcgacgtccg tcaccccctg ccccgaacgg
     7261 gacgtatctc cgagcgccgt tcggatcggc tgaagacgcc acgtcgcgcc cagatcgtcg
     7321 ctccgcagca acgcgtcaaa cgacccgatt gagccgccga caagcacttt ttgcccgttg
     7381 aacgcaaaac cccacgcccg gtaatccacc ccgacggtgg catcgtcaac cggcacgtac
     7441 gtgtgccagg tctgaccccc atccctcgtc cagcttaccc cgttctttcc gggcccgtcg
     7501 agtattccat cgcggtcaag atcgtaatca agcgaatcaa acacaccgtc cgtgaacaca
     7561 tcctgagggt tgttcttcag atagttctca tccagtcccg tagactgagt agacgcccag
     7621 aggattccat tgaccgtatc caccgcgacc gagggaacaa aatccccgct gatgcctgcc
     7681 ctggctgcgt caaagttctg ccacgtgacg ccgcgatccg tagaccggtt gatgcctgcg
     7741 ttggtggaaa cccagagcgt gtcatgcact gcgaggccat cgaatgcgcg gtaattcaga
     7801 tgccggtacc ggtgcagccg cccgggatcc ccggtaaatg gattcggctg ggggttgttg
     7861 gtggaatcgg gccggtaccg gtgccacgtg accccgaaat ccggcgtcat gacggcaaac
     7921 tcggtggtaa atgccgccca gaggtccgtg ccgtcaaacc acacgccgaa tgactgaaca
     7981 tcgccgcccg gaaccgccat gttcgttcga tcggcaaaaa gggccgtcat gggaatcgga
     8041 aaccagctcg caccgccgtc gcgggaaaga tacagaccgt cgcccacgta gtagctgccg
     8101 gacgagctgt tcacgacgtg cgaagtggcc gccgcaatgc gatccccact caccgcgtat
     8161 gccgtgatcg cctcatccgg gcgcgacgta atcgttccta cgtcgtacgt cacccagtcg
     8221 aactgcgata caccggaacc ggcaagccgg ctcaaagtgc ggcgtgcgct cacccacagc
     8281 gtcgtgtcgg cgcgggcgaa aaaggcggct tcgttgcttt ccagcccgat tcgcgcaccc
     8341 agcaccgtcg ccgggcgagg catctgcgcg tgcacaacgg tgttcgggaa caggcctgca
     8401 acgcccgcaa aggcaatgac gcggagaaac tgtctcgtgt tcagcggata ccttcctcgg
     8461 aaggggacac cggtttttcc ttcggaaaca tgctaaaaat accccgcgcc gctctcaaac
     8521 aacaacgacc ggccgcgctg gagacaaatg cgcccgcgtg gcacggaacc ataagaccgg
     8581 cgaacgcgct ttattcattc aagaccagcc cagacggaaa aaccctccgc cgaacagaaa
     8641 acgcgtcccg gcgagggcct cgacaatcgg gtagattttt cgggaatgat tcatgaaaca
     8701 ttcggttctt gtttttgtgc tgctcctcgt ttccgccatt tcggttgccg gccaggccat
     8761 cgaggaggac gccttcctgt taccggaccg cgtgcgcagc aatccggaca aattcacctt
     8821 cgccatcctg ggagacagaa ccggttccga ccgccagagc tggcgcattt tcgaccgctc
     8881 ggtagaggag atcaacctct cccgccccga tttcgtcatt atggtcggcg acatgattga
     8941 ggggcacgac tacccggaag aagtcgccgc gcagtgggag gaattccaga gccatgcgga
     9001 ccggctggaa gcgccgttca tgtt
//
LOCUS       DAOTNG010000113         8954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7078_length_8954_cov_5.105630, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000113 DAOTNG010000000
VERSION     DAOTNG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7078_length_8954_cov_5.105630"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="P5179_06925"
     CDS             <1..2143
                     /locus_tag="P5179_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS94985.1"
                     /translation="PVEGAIRWELFDTLRGRNYAQGALEPTGRDMWARWTWVADFTSL
                     RREGYYLLRVHMPDRIVESAPIRIWNGVYEHLAYTVAKYSYLQRCGVEIPGYHKPCHT
                     NDALWRSVAQDETYGKVLEYRDLRGGWHDAGDYNKWFHYFGYVLETLALMHKRVDLPK
                     QTYGGDIPDVLSEIFWGADFFLKVQNPDGSFVGPIHAWYTHVRPETGERYNSNWAIFW
                     GEKIEEDSGHGEVMHPRSRFYDYTSHNNGGLVLDLATALATAARCARGVDDPRRWTYA
                     NAALKSGAHVEKTYPGMAGHPYWVTFWYDLYRATDRPEYRENAFALVDPLLAQQAEDG
                     SFGRPAGLKHTFHPITVLMELLVDEPAHPAREKILRAVERKLAWMEPYTLGRPWDLAL
                     QCASGGERGELSGGTMGRNAWVGNVAYVYALAGRLTGRREWLHKSEAQLAWLLGRNPH
                     GVSQVVDAGRVNVGRYHGWSNHCENDQQGRLTGGIINGILTTDDAYPDSRNWTIMPPR
                     FPVLSVRREDVPYAEHAIHNARHDTNEYWSLHHAGFQQAVSALAAAYKGLEEPERPRL
                     AYLYRNFWSYDAATACDELFDRAGWNADRIASDSRYGHFDTSAYRAMVVSRSWTGSEF
                     IDAEPFGIQMRHSFLRGVPWIFLPPEDPVCLDWLEEFSSGQVPCGEVGKPEGTWEPCR
                     YGRVRTMHGAKVYLVNDEAALERLLVDLRTV"
     gene            2380..3360
                     /locus_tag="P5179_06930"
     CDS             2380..3360
                     /locus_tag="P5179_06930"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRS94986.1"
                     /translation="MQANTCVNPWGPIVLFAGRSIPDTARAIAKELGLELSKAMVTQF
                     PDTESYVLLQENVRGADVFIIQSTCAPVNETYMELLVMIDAVRRASASRITVIMPYMG
                     YARQDRKATGREPISAKLMADLVTTAGADRAVSVDLHNSAIQGFFHVPLDHLTAIPAM
                     AAELKKLSLIDPVLIAPDVGRAKLVEKFQEILHIPMAIAHKRRKGLDVTVTEIIGEVE
                     DKTPVIVDDIIASGSIVRHIDALVERGARPEIYMAITHPVLVGKAVERLGHPALKMLI
                     TTDTIPIPPEKQSPKIRVVSTAPMLADVIRRIHTNESVSEMFAKLFEFPV"
     gene            3445..4392
                     /locus_tag="P5179_06935"
     CDS             3445..4392
                     /locus_tag="P5179_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS94987.1"
                     /translation="MLAFGPIPSRRLGHSLGINNIPPKVCSFNCVYCQVGTTSEMAIE
                     RRVFYSTEQIVEAVGAKVNDARASGEKIDYLAFVPDGEPSLDINLGAHISALREFGIP
                     VAVISNSSLIWREDVRADLARADWVSLKVDSVREETWRKIDHSHGGLSLPAILDGARE
                     FASGFKGKLVSETMLVAHLNDSEEEMDAVGVFLAQLQPAMAYLMIPTRPPAKDWVEFP
                     DDAALVAAHRALSLRFPRVELLTKYEGDAFASLGDAGDALLGILAVHPMREDAVLSFL
                     ARAGADVNVLDQALQKGLVVKTTYKGQAFYVRRRAKDTR"
     gene            complement(4456..5859)
                     /locus_tag="P5179_06940"
     CDS             complement(4456..5859)
                     /locus_tag="P5179_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS94988.1"
                     /translation="MPERPNVLFLMNDHTNAEAVAPGSPCIKPNLDLLAREGIRFERC
                     HTTNAICSPARASLMTSLYPSTHGMWDCTHTQRPEWVDVPASRFTYFSTQLANAGYTN
                     GYFGKWHVEQSLRLEDFGWHEYDVNIGSMRHAPAEHGKVVVPKEGYRDYLLCGVDSSG
                     AEYTHPAFDRGCDFIRRTAADGKPFCCFVSTSEPHDPYVPPQRFLDLYDISSIPLSPT
                     LREEPSGKPEVVRRMRAEWSSLTDADWRRIRASYWAVITAVDAEIGRILNVLHETGQY
                     DNTIIVFLSDHGDMLGSHGLATKGVGTAYEEVHNVPLIVRGPGIKRGIEDCRTVTSLV
                     DLAPTVLDLCGADPLPRAQGRSLRPVLDGTADPADWEDAYAEFFGQRFVYTQRITWHG
                     NWKYVFSPGGVDELYNLASDPHERHNLAEDPAHREVLIDMCKRMWRKMKEIGDESLFN
                     THYATLRTAPIGPLSVM"
     gene            complement(5927..7498)
                     /locus_tag="P5179_06945"
     CDS             complement(5927..7498)
                     /locus_tag="P5179_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRS94989.1"
                     /translation="MGLRFGDAIAIALYVAAMVWLGWRSQRRIKGTEGYFVGNRAMPG
                     WAVGLSILATAISSVTFLAMPGNSYQGNWSRYVPGLMLPVATLIGVYFFVVFYRRTRF
                     VSAYQYFEKRFGNWGRSYASVLFSIGSLYRIGMVLYLMSIPIKVLFGGDILSIILFAG
                     IIATVYTVMGGLEAVIWTDVVQGGVLVLGGVLTIAIVFLDVPGGGAEIIHRASAAGKF
                     DMAASFDWSLSRETVWVFVLMGLVSNLQELATDQTKVQRYQAAKTDKGAIGATLTVIA
                     CLPVWAMFMFIGTCLWAYYSDFPNLLPAGIKADYVYPHFILTQMPTFVAGLVISAVLA
                     AAMSSVDSSMNGSSAALLEDFYKRHLAPGRTDTHYLRVARGITAALGILMMVIAWALS
                     LLQADTILEMWIFINAVVAGGLGGFFLLGFLFPHANDRGARIGVAAGVVAILWCTLSH
                     LNPQFGLLPGWIVLNVHPYLIGVVGNVVVLLVGIAASLFFAKPTEQQLAGMTWWTRDR
                     RPEQEVLATSVSSGL"
     gene            complement(7689..>8954)
                     /locus_tag="P5179_06950"
     CDS             complement(7689..>8954)
                     /locus_tag="P5179_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94990.1"
                     /translation="TMFVSAYEFFERRFGNWGRSYASVLFSIGSLYRMGIILYLMSIP
                     IRVVFGGSALTVILFAGIVVTVYTVMGGLEAVIWTDVVQGMVLILGGLLTVLVAFADV
                     PGGASEIIHRASMAGKFDIAVSFDWKLAEESFWVFVVLGLMNNVQELATDQTLIQRYQ
                     AARTDRGAIAAVLTQLACIPLWLLFMFVGTCLWVYYQHFPNLLPVGIKADFIYPHFIL
                     TQMPTFVAGVVISAVLAAAMSSVDSSMNASAAVLLSDFYKRHLAPNRTDTHYLKVARF
                     ITAGLGAAMMVVALGLSSLEASTILEVSFFVSAVITGGLGGFFLLGFLITRANDQGAR
                     IGVAAGVVSILWCTLSSLQPALGLLPTWAVIRVHPFLIGVVGNFVIFAVGVAASFFFP
                     RPTEQQLAGMTWWTRDRRSEKEALEQAGR"
BASE COUNT         1850 a         2617 c         2815 g         1672 t
ORIGIN      
        1 gcccgtggaa ggcgcgattc gctgggagct atttgacaca ctgcgcggcc ggaactacgc
       61 ccagggcgca ctcgagccca cgggtcgcga catgtgggcc cgctggacgt gggtcgctga
      121 tttcacgtcg ctgcgtcgcg agggatacta cctgctgcgt gtgcacatgc cggacaggat
      181 cgtcgaatcc gcgccgatcc gcatctggaa cggcgtatat gagcatctcg cctacacggt
      241 ggcgaagtac agctatctcc agcgctgcgg cgtagagatt cccggctacc acaagccctg
      301 ccacaccaac gacgcgctct ggcggagcgt tgcgcaggac gagacgtacg ggaaggtgct
      361 tgagtaccgc gacctgcggg gcggctggca cgatgcgggc gactacaaca agtggttcca
      421 ctatttcggg tatgtgcttg aaacgctggc gctgatgcac aaacgggtgg atctgccgaa
      481 acagacctat ggcggcgaca tccccgatgt gctgtcagaa atattctggg gtgcggattt
      541 cttcctgaag gtgcagaacc ccgacggctc attcgtcggg ccgatccacg cgtggtacac
      601 ccacgtgcgc ccagaaaccg gcgaacggta caactcgaac tgggcgatct tctgggggga
      661 aaagatcgag gaggactccg gccacggaga ggtcatgcac ccgcgcagcc ggttttacga
      721 ctacaccagc cacaacaacg gtggcctggt gctcgatctg gccaccgcgc ttgccaccgc
      781 cgcccggtgt gcacggggag tagacgatcc gcgccggtgg acctacgcca acgcggcgct
      841 caagagcggc gcgcacgtgg aaaaaaccta cccaggcatg gcggggcacc cgtactgggt
      901 gacgttctgg tacgacctct accgcgccac cgatcggccg gagtaccgtg aaaacgcgtt
      961 cgcccttgtg gacccgttgc tagcccagca ggcggaagac gggtcattcg ggcggcccgc
     1021 ggggctcaaa catacgttcc acccgatcac ggtgttgatg gagctccttg tggacgagcc
     1081 ggcacatccg gcgcgggaga agatactgcg ggcagtcgaa cgcaaactgg cgtggatgga
     1141 gccgtacacc ctcggccggc cgtgggatct tgccctgcag tgcgccagtg gcggcgagag
     1201 gggtgaactc agcggcggta caatggggcg caatgcgtgg gttggcaacg tcgcgtatgt
     1261 gtatgcgctg gcgggccgtc tcaccggcag gcgtgaatgg cttcacaaat cggaagccca
     1321 gctcgcctgg ctgctcggtc gcaacccgca cggcgtctcg caggtggtgg acgccggcag
     1381 ggtgaacgtc gggcgctacc acggatggtc gaaccactgc gaaaacgatc agcagggacg
     1441 gctcaccggc gggatcatca acggaatcct gacgaccgac gatgcgtatc cggacagcag
     1501 aaactggacg atcatgcctc ccaggttccc cgtgttgagc gtgcgcaggg aggatgtgcc
     1561 gtacgccgaa cacgcgatac acaacgcccg ccatgacacg aacgagtact ggtcactgca
     1621 ccacgcaggg tttcagcagg cggtcagcgc ccttgcggcg gcgtacaagg ggctggaaga
     1681 gccggaacga ccgcgactgg cgtacctcta ccgcaatttc tggtcgtacg atgcggcgac
     1741 cgcatgtgac gagcttttcg atcgcgccgg ctggaatgcg gatcgcattg ccagtgacag
     1801 ccgttacggg cacttcgata cgtctgcgta ccgggcgatg gtggtgagca gatcgtggac
     1861 gggcagtgag ttcatcgacg ccgaaccgtt cggcatccaa atgcgacact cgttccttcg
     1921 tggcgtcccc tggatttttc ttccgcctga agaccctgtc tgtctcgact ggctggagga
     1981 gttttcttcg gggcaggtcc cgtgcgggga agtcgggaag ccggaaggaa cgtgggagcc
     2041 gtgccggtat ggcagggtaa gaaccatgca cggcgcaaag gtgtacctgg tgaatgacga
     2101 ggctgcgctg gagcgccttc tggtggacct caggacggtc tgacgcaccg ggaggcttcg
     2161 ccgacatgcg tgcgccgctt ccgggggaat tgtcggagag gtgtgagtct gctcgtgttt
     2221 cgcgcacttg tgtcgtgctc cattgaccgc tccgctctga gtgccgattt tcattactcg
     2281 gggcaatgtc tctccgcccc ctgtttctag ttcgacggaa gccgtgtttc acgtggtgcc
     2341 tcgtgccgta tcggggtgcg cgacgcagaa ggaaaagccg tgcaagcaaa tacgtgtgtg
     2401 aacccgtggg gaccgatcgt gttgttcgcc gggcgttcca taccggatac cgcccgggcc
     2461 atcgccaaag agctggggct cgaactcagc aaagccatgg tgacgcagtt ccccgacacg
     2521 gagagctatg tcctgctgca ggaaaacgtg cgaggggctg acgtatttat cattcagtcc
     2581 acgtgtgcgc cggtgaatga gacgtacatg gaactgctcg tcatgatcga cgccgtacgt
     2641 cgcgcgtcgg catcgcgcat cacggttatc atgccttaca tggggtacgc aaggcaggac
     2701 cgcaaagcta cgggccgcga accgatcagc gcaaagctca tggcggatct cgtcaccacc
     2761 gctggagccg accgcgcggt gtcggtggac cttcacaact cggcaatcca ggggttcttt
     2821 catgttccgc tggaccatct cacggctatt cctgccatgg cggcggagct gaagaagctg
     2881 agcctgattg acccggtgct tattgccccg gacgtcggcc gcgcgaagct ggtggagaag
     2941 ttccaggaaa tcctccacat tcccatggcg attgcgcata agcggcgaaa aggtctcgac
     3001 gtgacggtga cggagatcat cggcgaggtt gaagacaaaa cgccggtcat agtggacgat
     3061 atcatcgcca gcgggagtat tgttcgccac attgacgcgc tggtggagcg aggtgcgcga
     3121 ccggaaatct acatggcgat cacccatccc gtgcttgtgg ggaaagccgt ggaacggctt
     3181 ggccacccgg cgttgaagat gcttatcacg accgacacga taccgattcc tccggagaaa
     3241 caatcgccga agattcgcgt tgtcagcacg gcaccgatgc tggctgacgt tatccggagg
     3301 attcatacga acgagtcggt gtcggagatg ttcgcaaagc tgtttgaatt ccccgtctga
     3361 gcaatcttcg caaggctgca gtatccttca tcgggaggac gccaaggcat ttcgcagtcc
     3421 tcccgagcca tccggagtgc caccatgctc gcatttggtc caataccttc gaggcgcctc
     3481 ggccacagcc tcgggatcaa taacattccg cccaaggtct gttcgttcaa ctgcgtgtac
     3541 tgccaggtcg gcaccacctc cgagatggcg atcgagcggc gggtttttta ctccacggag
     3601 cagatagttg aggcggtggg cgcgaaagtg aacgatgccc gcgcgtcggg agaaaagatc
     3661 gattacctcg cgttcgtgcc cgatggagag ccgtcgctgg atatcaatct gggtgctcac
     3721 atttctgcgt tgcgggagtt tggtatcccg gttgcggtga taagcaattc gtccctgatc
     3781 tggcgggagg atgtgcgagc ggaccttgcc agggccgact gggtgtccct caaagtggat
     3841 tcggtcaggg aggaaacctg gaggaaaatc gaccattccc acggcgggct gtcacttccc
     3901 gccattctgg atggggcgcg tgagtttgct tccgggttca aagggaagct tgtttcggaa
     3961 acgatgctgg tggcccacct gaacgacagc gaagaggaaa tggacgcggt aggagtattt
     4021 ctcgcgcagt tacagccggc aatggcgtat ctgatgattc cgacacgccc gccggcaaag
     4081 gactgggtcg agttccctga tgatgccgcg cttgtggccg cgcaccgcgc gctgagcctt
     4141 cggtttccgc gagtggagct gctgacgaag tacgaagggg acgcattcgc atcactggga
     4201 gatgcgggcg acgcgctgct gggaattctt gccgtgcatc ccatgcgaga ggatgcggtt
     4261 ttgtcgttcc ttgcacgcgc cggtgcggat gtcaatgtgc ttgatcaggc cctgcagaag
     4321 gggcttgtgg tcaaaaccac gtacaaaggg caggcgttct atgtgagacg ccgcgcgaaa
     4381 gacacgcggt gagatttgaa gaccgcagga acagaaaagg agggcgcgca tgtggcgccc
     4441 tccttttttt ggtgatcaca tgacagacag cggaccgatc ggggcggttc gcaacgtggc
     4501 gtaatgcgtg ttgaaaaggg actcgtcgcc gatttccttc attttgcgcc acatgcgctt
     4561 gcacatgtcg ataagcacct cccgatgagc agggtcctcg gcgaggttgt ggcgctcgtg
     4621 cgggtcggat gcaaggttgt acagctcgtc caccccgccg gggctgaaga catacttcca
     4681 gttcccgtgc caggtgatgc gctgggtgta gacgaatcgt tgcccgaaga attctgcgta
     4741 cgcgtcctcc cagtctgccg ggtcagcggt gccgtcgagc accgggcgca gtgatctgcc
     4801 ttgggcgcgg ggaagtggat ccgcaccgca cagatcaaga accgtcggcg caagatcgac
     4861 cagactggtc actgtacggc agtcctcaat ccctcgtttg attccgggtc ctcgcacgat
     4921 cagcgggaca ttgtgcactt cctcgtatgc agttccaacg cccttcgtgg ccagcccgtg
     4981 ggaacccagc atatcgccgt ggtcgctcag aaatacgatg atggtgttgt cgtactggcc
     5041 tgtttcgtgg agcacattca ggatcctgcc gatctccgca tctaccgcag tgatgactgc
     5101 ccagtaggag gcacggattc tccgccagtc ggcgtcggtg aggctgctcc actcggcccg
     5161 catgcgtcga actacttctg gtttgcctga gggctcctct cggagcgtgg ggctcaacgg
     5221 gatactgctg atgtcgtaga gatcgaggaa gcgctggggc ggtacgtaag ggtcgtgcgg
     5281 ttccgaggtg gaaacaaagc agcagaacgg tttcccgtcg gctgccgtgc gtcgtatgaa
     5341 atcgcagcct ctgtcaaacg cggggtgcgt gtactccgct cccgagctgt ccacgccgca
     5401 cagcaggtaa tcgcggtagc cttccttcgg cacaacaacc ttcccgtgtt ccgccggtgc
     5461 gtgccgcatg ctgccgatat tcacgtcata ctcgtgccag ccgaagtctt caagcctgag
     5521 cgattgctcg acgtgccatt tcccgaagta gccgttcgtg taccctgcgt tggccagttg
     5581 agtggagaaa taggtgaagc ggcttgccgg aacgtccacc cattcgggcc gttgagtgtg
     5641 cgtgcaatcc cacatgccgt gcgtcgacgg gtacagcgat gtcatgagcg atgcgcgcgc
     5701 gggcgagcag atggcgttcg tcgtgtgaca tcgttcaaag cggataccct cgcgcgccag
     5761 cagatcaagg ttcggcttga tgcacggact gccgggggca actgcctcgg cgttggtgtg
     5821 gtcgttcatg aggaacagaa cgttggggcg ttctggcatg gtcagtctcc cggtgcagaa
     5881 ggtgaaaatc gccgattcct gcaaagtgta aggcggatca gtgctgtcat agaccggatg
     5941 agacggatgt agcgagcacc tcttgttcag ggcgcctgtc gcgcgtccac cacgtcatgc
     6001 cggcgagctg ctgttccgtt ggtttcgcga agaacaggct tgcggctatt ccgacaagca
     6061 gcaccaccac gttgccgacc accccgatga ggtaggggtg tacgttcaga acaatccagc
     6121 cggggagcaa cccgaactgc gggttcaggt gactcagggt gcaccagagg atcgcaacta
     6181 cccccgcagc cacgccgatt ctggctcctc tgtcgtttgc atgcgggaag aggaagccca
     6241 gcaggaaaaa gccgccaagg ccgcccgcca ccactgcgtt gatgaatatc cacatttcga
     6301 ggatggtatc ggcctggagc agggaaagcg cccacgcgat caccatcatc aggatgccca
     6361 gcgccgccgt gatgccacgg gcgacgcgaa gataatgcgt gtcggtgcgc cccggggcga
     6421 gatgccgctt gtagaagtcc tcaagcaggg ccgccgaaga tccgttcatg ctggagtcga
     6481 cgctcgacat ggcggcggca agaacggccg agataacaag ccccgcgacg aaagtcggca
     6541 tctgcgtcag gatgaagtgg ggatagacgt aatcggcctt gattccggcg ggaaggaggt
     6601 tcgggaagtc tgagtagtat gcccacaggc aggtcccgat gaacatgaac attgcccaca
     6661 ccgggagaca ggcgatcacc gtcaacgtgg cgccgatagc gcctttatcg gtttttgccg
     6721 cctggtaccg ctgcactttc gtctgatcgg tcgccagttc ctgcagattg ctcaccaatc
     6781 ccatcaaaac gaatacccag actgtctccc ggctaaggct ccaatcgaac gaagccgcca
     6841 tgtcgaattt cccggctgct gacgctcggt ggatgatctc tgcgccaccg cccggcacat
     6901 cgaggaacac gatggctatg gtgagcacgc cgcccagaac aagaacgccc ccctgaacca
     6961 cgtcggtcca gatcaccgcc tcaagcccac ccatcacggt gtacaccgtc gcgatgatgc
     7021 cggcgaacag gatgatggag aggatgtctc ccccgaacag gaccttgatc gggatggaca
     7081 tcaggtacag caccatgcct atgcggtaga gagatcctat tgagaacaat acacttgcgt
     7141 aggagcgacc ccagttgcca aagcgtttct cgaaatactg gtacgcgctg acgaagcgtg
     7201 tgcggcggta gaagacgacg aagaaataca ccccgatgag ggtggccaca ggaagcatca
     7261 ggccggggac ataacgcgac cagttgccct ggtaggaatt cccgggcatt gcgaggaacg
     7321 taaccgagct tatcgccgtc gccaggatcg aaaggccaac cgcccacccc ggcatcgccc
     7381 gattgccgac gaaataccct tcggttcctt tgatccggcg ctgagatcgc cagccaagcc
     7441 acaccattgc ggcgacgtag agcgcaatgg cgattgcatc cccgaaccga agccccatcg
     7501 gctacctccc cgaacgaatg gttcctcgaa cgcgcgagcg gtattcacgt gcaaggcagg
     7561 aaacaacaag atcaccccgc gctgcgcggt ggcgcaacag catggcgctg ccggagattt
     7621 ccgttgtccg cggttggatc cggcacatgg agcgaggctt gcttcgtcct gagcgcacgc
     7681 acgggaaatc agcggcctgc ctgctccagc gcttcctttt ctgaacggcg gtcgcgcgtc
     7741 caccacgtca ttccggcgag ttgctgttct gtcggacgcg ggaagaagaa gctcgcggcg
     7801 acgccaactg cgaaaatgac gaaatttccc accacgccga tcaggaacgg gtgcacccgg
     7861 atcacggccc acgtcgggag caaccccaga gcaggctgaa gactgctgag cgtgcaccag
     7921 agaattgaga ccacgcccgc cgcaacacct atccgtgcac cctgatcgtt tgcgcgcgtg
     7981 atgaggaacc cgaggaggaa gaacccgccc aggcctccgg ttatcaccgc gctgacgaaa
     8041 aagctgactt cgaggatcgt gctggcttca agcgatgaga gtccgagcgc aaccaccatc
     8101 atcgcagcgc ccagtcctgc ggtgatgaat cgggcaactt tgaggtagtg cgtgtcggtc
     8161 cggttcggcg caaggtgtcg tttgtagaaa tccgacagca gaactgccgc cgaggcgttc
     8221 atgctggaat cgacactcga cattgcggca gccagcacgg cggagatcac aactcccgcc
     8281 acaaaggtgg gcatctgggt gaggatgaag tggggataga tgaaatccgc cttgatcccc
     8341 accggaagga ggttcgggaa atgctggtaa tacacccaga ggcaggttcc gacgaacatg
     8401 aacaaaagcc agagcggaat gcacgccaac tgggtgagca cggccgctat tgctcccctg
     8461 tctgttctgg cggcctggta gcgctgaatg agcgtctggt cggtggcgag ttcctgcacg
     8521 ttgttcatca gcccgaggac gacgaacacc cagaacgact cttcagcgag tttccagtcg
     8581 aacgaaacgg cgatatcgaa cttgcccgcc atggaggctc gatggatgat ctcagacgcg
     8641 ccgcccggca catctgcaaa ggccaccagc acggtgagca gaccacccag gatcagaacc
     8701 atcccctgga cgacgtccgt ccagattacg gcttcgaggc ctcccatgac ggtatacacg
     8761 gtaaccacga tgccagcgaa aaggatcacg gtcaacgcgc ttccgccgaa caccacacga
     8821 atcgggatgg acatcaaata caggattatt cccatccggt acagcgagcc gatcgaaaaa
     8881 agtacgctcg catacgagcg cccccagttg ccgaaacgtc gttcgaagaa ctcgtacgcg
     8941 ctgacgaaca tggt
//
LOCUS       DAOTNG010000114         8897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7156_length_8897_cov_4.855915, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000114 DAOTNG010000000
VERSION     DAOTNG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_8897_cov_4.855915"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1512
                     /locus_tag="P5179_06955"
     CDS             208..1512
                     /locus_tag="P5179_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS94991.1"
                     /translation="MNKLEISPPEPPCPSAYDVVVIGGGTAGVVAAMQAALDGVSTLV
                     VEKTGQLGGTMTNAGIAFPGLFHAWGRQVIAGIGWNLVRRCVEESGGALPDFAKPPRH
                     HSDHQIPLDRALFTALCNKAVRESGSRVMLHTMLADVTEHPDGWDIVLCTKTGLRQMS
                     ARVLVDCTGDANAVALAGGGVHVRRDTQPATLSFRLSGFDLSTLDPEALNLRALDAMR
                     AGLLEPTDAGWDPNAPDVAGVLRANGRNSNHIHAAGACTSEGRTELEMSARRSFLRLF
                     RFLRAQPGLENLRVEYLAAECGVRESAVIAGDVVVTADDYVSGRVWPDSLCHAFYPID
                     LHTREGSGLDCRPLEEGVVPTVPLAALTARGKRNLLAAGRCLSSDRLANSALRVQATC
                     MATGQVAGAAAALAAKTGCDVREVGLEDIRSLLRQHGPIVPA"
     gene            1548..3950
                     /locus_tag="P5179_06960"
     CDS             1548..3950
                     /locus_tag="P5179_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRS94992.1"
                     /translation="MDGAPQPPRRTALRPAVWAVAFWIAGELCGFEWGGSPGLLCAGA
                     FLFAVLAGVALVRQKRGIVALAALSGACVLLGWGRVALWQETRPSPAILSRFFDQRVV
                     LSGTLTSTPEPRGRVWRFGLKADTLASAENAVLGPVDVFVRMDTSLGAPRIGQRVRIA
                     GSVRALPRRSAPGTMDYGEYLRYRGYSATLAAYEPGGFAPLPVPRNAWTAFWDHARRY
                     VKRAITVGMPDEAAGLASGLVLGERHGLSRRTMEAFSACGVIHILSVSGFHVAIVLVG
                     IGVPLRRFFGFGLWQALVLIACAWCYSQLAYNDPPMVRSCIMATVLLLAYTVERRSDP
                     WNALALSALITLLVQPTALHDIGFQLSYAAMAGLMGFGSMAEERLKRFRFLQQHAWVR
                     YPISLAVATVAANITTAPLTAHYFWRMSLLALVGNLPITALVTVGVWASVAAALVGWI
                     PFAAIAINAANARVLEAVLRLGDWFAEWPYAATWVRSPTWPEMLVYGVGTCAIWWISQ
                     RKGLRKGALFACLIYANIAVWWIWIHRPGTTEIAFLDVGQGDAAVCRFSTGHTIVIDG
                     GPATAGYDAGDWVVVPYLRSRGIRRVDAIVVTHSDSDHIGGLPSVAQAFDTRAVWYNG
                     SGDTASAFRALESNIRQRGGRLQVAGAGDSVAGLGTAQMTFLSPMRDSAWSSRCSDND
                     RSLVLRIVEYGDTVLFTGDTGEKAERAYSRLLPTESGARVDVLKVAHHGSRFSTGETL
                     LATARPRYAVISVGRNMYGHPARETLEQLRATGTTVFRTDRQGTLIWRTGPAGGRWVE
                     WR"
     gene            complement(4005..4625)
                     /locus_tag="P5179_06965"
     CDS             complement(4005..4625)
                     /locus_tag="P5179_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS94993.1"
                     /translation="MKHGTVAAAYLVAAMFVSALQVHAQEPPDQQARPARMRDAVVIG
                     EQQATIRLMPLKDPNLAAILALGTPGMGHFYVGNWKRGAAFLTGVMGSVVAVGLAGDN
                     LALTVEDYDTPARGGNANGVVEVAEYRAWEKKRTRDLSEISTARKAVMLTGLTSALGL
                     YVWNIFDAHSQAEDYNRQLYRDLTGVRVGLCPGPHGMRGMMSVPIR"
     gene            complement(4724..5923)
                     /locus_tag="P5179_06970"
     CDS             complement(4724..5923)
                     /locus_tag="P5179_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS94994.1"
                     /translation="MRFRAARWRALPTAIWALGFGSLFMDISSELIHSLLPVFMVSSL
                     GASMVVVGVVEGVAEATAAFVKLFSGALSDWFGRRKPLLVLGYALGAASKPVFPLAGS
                     VAWVFAARFVDRVGKGIRGAPRDALVAEVAPANMRGAAYGLRQALDSVGSLAGPLLAV
                     PLMVWFLGDIRSVLWVATFPAVVSVLVLIAYVREPERVEVRGAARPRLMFAGVGRLPG
                     RYWIVVLLGAVFTLARFSEAFLVLRARDTGMPDGRVPLVLVVMNLFYAAGAYPAGSAA
                     DRINRRVLLLGGLALLVASNLVLAGATTPPTVLFGVALWGLHMALTQGLLAKLVSDAT
                     PGELLGTAFGVFHLVSGAMLLVASIVAGALWTTQGASATFLAGAAFAAIAAIGVWSVA
                     RRSAPEP"
     gene            6057..8048
                     /locus_tag="P5179_06975"
     CDS             6057..8048
                     /locus_tag="P5179_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRS94995.1"
                     /translation="MVLLQLDKISHSYGANEILHRITWQIKDDTRAGLIGPNGCGKTT
                     LARIITGEIAPSSGTITQSGTVSIGYLRQESAAPPGHTVWSYIAGADARLADLRTRLA
                     ALEETIARDYQSPADSSRGDVPPDLIAAYGAAHDEYEHMGGYEYDARCASVLSRMGFR
                     AEDYDQPVELLSGGQQSRLTLAALLVRQPDVLVLDEPTNHLDIEATEWLEGFLSSYKG
                     AVIVISHDRVFLDRVVTEIVEIERMRLVHYRGNYSAYAAQKEERRQQETKAYELQREM
                     IERTEEFIRRNIAGQKTKQAQGRRKQLEKLERLTPPPFQRSVSLSFVASHRGGNEVIE
                     VRSLSKAYNGLPLFEELSFLVRRGERVGVVGPNGSGKSTLLRMLVGEEEPDGGIIRFG
                     VGVEAGYYDQRREGLDQESRVIDEVWSVKPSLTTEQIRSYLGSFLFTEEEQFRIIGTL
                     SGGEQSRVALAKLVLAQVNLLILDEPTNHLDIPARLALENALDRYDGTLLVVSHDRAF
                     LRRLTTRVIRIQDGSAQVFDMGYRALEAAQAEAREKEAREAVARGLATDEAAPRQVVA
                     ETSPADKAARLAAYERKRELERRRRQRERRFADIEAEIMDVENRLMEIDDELGNPAHA
                     ADWEMLRTLTAERGLAKEKLDSLYEQWAEMERHSSAGLD"
     gene            8173..8394
                     /locus_tag="P5179_06980"
     CDS             8173..8394
                     /locus_tag="P5179_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRS94996.1"
                     /translation="MKYRFVAVIEKDEDGYYAFCPELQGCYTSGDTFEEARANLADAI
                     RLHLADRLATGETFRTPESINMNTLEIAV"
     gene            8391..8564
                     /locus_tag="P5179_06985"
     CDS             8391..8564
                     /locus_tag="P5179_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRS94997.1"
                     /translation="MSDRLPRITAKDIIRVLGRHGFSLTRSSGSHQIFKNQAGARVTV
                     PVHTEKALHPKVL"
BASE COUNT         1676 a         2777 c         2852 g         1592 t
ORIGIN      
        1 ggcctttcgc cgctcgccac tcgacgaccg gtcagggaag cgcgcgcatc catacggcgc
       61 ggttgggcaa aggtgagcag ggaacacgcc ggccacgcga aaaatgccag ttcgtcgagt
      121 gattggcggg catttgatcc gccattcgta tcgagacgcc gggcgggctg gacaatacca
      181 gcgcccgcca cggatcaggg caggacgatg aacaaactcg agatatcccc accagaacca
      241 ccctgtccct ctgcgtacga tgtcgtcgtc atcggtggcg gcaccgccgg ggttgtcgcc
      301 gcgatgcagg cggcgctgga cggcgtctca acactggtgg tggaaaaaac ggggcaactc
      361 gggggcacga tgaccaacgc cggcatcgcc ttccccggtc tgttccacgc atggggccga
      421 caggtgatcg ccggaatcgg ctggaacctt gtccgccggt gcgtggagga atccggcggc
      481 gcgctccccg atttcgccaa gcccccgcgg caccattccg accatcagat ccccctcgac
      541 cgcgccttgt tcacagcgct ctgcaacaaa gcagtgcgag aatccggctc acgggtgatg
      601 ctccacacca tgctggcaga cgtgacggaa catcccgatg ggtgggatat tgtcctctgc
      661 acgaagaccg gcctccgcca aatgagcgct cgcgtgctgg tggattgcac aggggacgcc
      721 aacgccgtgg cgcttgcagg tggtggagtg catgtgcgga gggataccca gccggcaacg
      781 ctttctttcc ggctttcagg gttcgatctg tctacgctcg accctgaagc cttgaacctc
      841 cgggcgctgg acgcgatgag agcgggcctg ctggagccga ccgatgcggg atgggatccg
      901 aatgcccccg acgtggcggg ggtactgcgg gcaaacgggc ggaattccaa ccacattcac
      961 gcagccggcg cttgtaccag tgaaggtagg accgagctcg aaatgtctgc gcggcgatcg
     1021 ttcctacgcc tgttccgctt ccttcgcgct cagccggggc ttgagaatct acgcgtggag
     1081 tacctcgcgg cggagtgcgg cgtccgcgaa tccgcggtca tcgccggcga cgtggtggtc
     1141 acagccgacg actatgtctc gggacgcgtg tggccggatt cgctctgtca tgcgttctac
     1201 ccgattgacc tgcacacgcg ggaagggagc ggccttgatt gccgcccgct ggaagaaggc
     1261 gtcgtgccga cggtgccgct ggcggcgttg acggccaggg ggaagaggaa cctgctggcg
     1321 gcaggacggt gcctttcgag cgatcggctg gcgaactccg cgctccgcgt gcaggcgacg
     1381 tgcatggcga cagggcaggt cgccggagcg gctgcggcgc tggcggcgaa aaccgggtgc
     1441 gacgtgcggg aggtggggtt ggaggacatc cgctccctgc tacgccaaca cggaccgatt
     1501 gtcccggcgt aacagtgctc aaaccctttc gatcgggtcc ggatggtatg gacggggctc
     1561 cgcaaccacc ccggaggact gctctccggc ccgccgtctg ggcggtagcg ttctggattg
     1621 cgggagaact ctgcggattt gagtggggcg gttcgccggg cctcctgtgt gccggggcgt
     1681 ttttgttcgc cgtgctggct ggcgtggccc ttgttcgcca gaaacgaggc atcgttgccc
     1741 tggctgcgct gagcggcgcg tgcgttctcc tcgggtgggg ccgcgtcgcg ctctggcagg
     1801 aaacccgccc atctccggcc attctttccc gctttttcga ccagcgcgtc gttctctccg
     1861 ggacgctcac cagcactcca gagccgcgtg ggcgcgtgtg gcggttcggc ctgaaggcgg
     1921 acacgctcgc ttcggcggag aacgcggtgc tggggcccgt tgacgtgttc gtgcggatgg
     1981 atacctccct tggcgctccc cgcattggtc agcgcgtgcg gatcgccggc tcagtgcgag
     2041 ctctgccccg tcgttccgcg cccggaacca tggactatgg cgaatacctc cgctatcgcg
     2101 gatactcagc gacgcttgcc gcgtacgaac cgggcggatt tgctccgctg ccggtgccac
     2161 gaaacgcgtg gaccgcattc tgggaccacg cgcggagata cgtcaagcgt gcgattacag
     2221 tcggaatgcc ggacgaggcc gccgggctcg cgtcgggact ggtgctggga gaacgccacg
     2281 ggctctcgcg ccggacgatg gaggcattca gcgcgtgcgg agtgatccac atcctttccg
     2341 tgagcggatt ccacgtcgcc atcgtgctgg tggggatcgg ggtgccgctc cgccggtttt
     2401 tcgggttcgg gctctggcag gcgttggtgc tgatcgcgtg cgcatggtgc tactcgcagc
     2461 ttgcgtacaa cgacccgccc atggtccgct cgtgcatcat ggcgacggtg cttctgcttg
     2521 cctacacggt ggaacgtcgc tccgacccgt ggaacgcgct tgccctcagc gcgctcatca
     2581 cactgctcgt ccagcccacg gcgcttcacg acatcgggtt ccagctctcc tacgcagcca
     2641 tggcgggcct gatgggcttc ggctcgatgg cggaagagcg actcaaacgg ttccggttcc
     2701 tccagcagca tgcgtgggtg cggtatccca tcagcctcgc cgtcgcgaca gttgcggcca
     2761 acatcaccac tgcgccgctc accgcacatt acttttggcg tatgtcgctg ctcgcgcttg
     2821 tcggaaacct tccgatcacg gcgcttgtaa cggtgggcgt gtgggcaagc gtggcagcgg
     2881 cgctcgtggg atggattcca ttcgctgcga ttgcgatcaa tgccgcaaat gcacgtgtcc
     2941 tcgaggcagt gctgcggctc ggagattggt tcgctgagtg gccctacgcg gccacctggg
     3001 ttcgttcccc cacatggccg gagatgcttg tctacggcgt gggcacctgc gcgatctggt
     3061 ggatttcaca gagaaagggt ctgcggaaag gcgcgctgtt cgcctgcctc atctacgcga
     3121 acattgccgt gtggtggata tggatccacc ggcccgggac gacggaaatc gcgttcctcg
     3181 acgtgggaca gggcgacgcc gcggtgtgcc gattttccac cgggcacaca atcgtgattg
     3241 acggaggacc cgcaacagcc ggctacgacg ccggtgactg ggtggttgtg ccgtatcttc
     3301 ggtcgcgcgg cattcgccgg gtggatgcca tcgtggttac gcacagcgac agcgatcata
     3361 tcggtggcct gccctcagtt gcgcaagcat tcgatacacg tgcggtctgg tacaacgggt
     3421 cgggcgacac cgcgtcggcg tttcgagcgc tggaatcgaa catccggcag cgtggggggc
     3481 ggcttcaggt tgcaggggca ggtgattcgg tcgcggggct gggtaccgcg cagatgacat
     3541 tcctgtcccc gatgcgcgat tcagcctggt cctcgcgctg ttccgacaac gatcgctccc
     3601 tcgtgctgcg gatcgtggag tacggcgata cggtgctttt caccggcgac acaggcgaga
     3661 aagcggagcg ggcgtacagc cgactccttc ctacggagtc gggtgcgcgc gtggatgtcc
     3721 tcaaggtggc tcatcacgga tcccgcttct ccaccggaga gaccctgctg gcaaccgccc
     3781 ggccgcggta cgccgttatc agcgtcgggc gaaacatgta cggtcacccc gcacgggaga
     3841 cgttggagca gctccgcgcg accggcacca ccgtgtttcg aacggatcgg cagggcacgc
     3901 tcatctggag aacgggaccg gcaggaggga ggtgggttga atggagatga aaggggcgca
     3961 gagaccaaac caatgcccct gcgccccggt tcaaccaccc actgctaacg aatcggaacc
     4021 gacatcatcc ctcgcatacc gtgcggcccg gggcagagcc cgactcgtac gccggtgagg
     4081 tctcgataaa gctgacggtt gtaatcctcc gcctgagagt gagcgtcgaa gatgttccag
     4141 acgtagagac cgagcgcgct cgtgagcccg gtcagcatca cggcctttcg cgcggtggat
     4201 atctcgctca ggtctcgcgt ccttttcttt tcccatgcgc ggtattccgc aacctccact
     4261 acgccgttgg cgttgccccc gcgggccggc gtgtcgtagt cctcgacggt cagcgccagg
     4321 ttatcgccgg ccagacccac cgcaacgacg gatcccatga caccggtgag gaatgcggct
     4381 ccccgtttcc agttgccgac gtagaaatga cccatgcccg gtgttcccag cgcgagaatc
     4441 gcggcaaggt tcgggtcttt gagcggcatg aggcggatgg tggcctgctg ttcgccgatc
     4501 accaccgcat cacgcatacg cgcgggtcgg gcctgttgat cgggtggttc ctgcgcgtgc
     4561 acctgtaacg cactcacaaa catcgcggct acgaggtagg ccgcggcgac ggttccgtgt
     4621 ttcatcttct ccctccttgc gaggtgctcc cgcaacttca catactgtct ttttcactct
     4681 ccgcgaaccg gacggcggac atcttcgcac cattctgaac cggtcagggc tccggcgcgg
     4741 aacggcgtgc gacgctccac accccgatgg ctgcgattgc ggcaaacgct gcaccggcaa
     4801 gaaacgtggc ggacgcgcct tgcgtcgtcc acagcgcgcc ggcgacaatg ctcgccacaa
     4861 gcagcattgc ccctgacacc agatgaaaca caccaaaagc cgtgccaagg agttcgcccg
     4921 gagttgcgtc tgaaacgagt tttgcgagca atccctgcgt caacgccata tgaagacccc
     4981 acagcgcgac gccgaacagc accgttggag gggttgttgc gccggccagc acgaggttgg
     5041 aagcaaccag cagcgccagc ccgccgagaa gaagaacccg gcgattgatg cggtcagccg
     5101 ctgatcctgc cgggtacgca cctgccgcat agaacaggtt catgacaacc agcaccagtg
     5161 gaacccgtcc gtccggcatc ccggtatccc gcgcccgcag cacaaggaat gcctcgctga
     5221 agcgggccag cgtgaatacg gcccccagca gaacgacaat ccagtaccgc ccgggcaaac
     5281 gccccacgcc ggcgaacatc aggcgcggcc tggctgcgcc gcgtacctcc acgcgttcgg
     5341 gctcacgcac atacgcgatg agcaccagca cggatacgac cgccgggaag gtggccaccc
     5401 acagcacgga acggatgtcg ccgaggaacc agaccatcag cggtaccgcg agcaagggcc
     5461 ctgccagcga gccgaccgaa tcgagcgcct gacggagccc gtacgccgcg ccacgcatgt
     5521 tcgcgggagc cacctccgcg accagcgcat ccctgggcgc gcccctgatg cctttcccga
     5581 ctctgtcaac aaaacgagcg gcaaacaccc atgcaacgga gcccgcaagc ggaaataccg
     5641 gtttgcttgc ggctccgagt gcgtacccga gaaccagcag aggtttgcga cgcccgaacc
     5701 aatcgctcag agcaccggaa aagagcttca cgaaggcggc ggtcgcctcg gctacgcctt
     5761 cgacgacacc caccacaacc atggatgcgc ccagcgaact taccatgaac accggaagaa
     5821 ggctgtggat gagttcggac gagatgtcca tgaacaacga gccgaacccg agcgcccaga
     5881 tcgctgtggg aagagcgcgc cagcgggccg ctcggaatct catgttcgcc acctgaacct
     5941 cccggccctt tgatacacgg aaccgaaaca acgcgcgttt cccgggtcac tcattcgtat
     6001 tttctgtaca gcaggtcgtt cggtactctt ttgccctcgc acaggtacac tccggaatgg
     6061 ttcttctcca gcttgacaaa atctcccact catacggcgc gaatgaaatc ctccaccgca
     6121 tcacgtggca gatcaaagat gatacacgcg ccggtctgat tggccctaac gggtgcggca
     6181 aaactacgct tgcacgtatc atcaccggcg agatcgcacc ttcgagcggc acgattacgc
     6241 aatcgggtac tgtgtctatc ggctatctca ggcaggaatc agccgcgccg ccgggccata
     6301 ccgtctggag ctatatcgcc ggggcagacg cccgtcttgc ggacctgcgt actcgcctcg
     6361 ccgccctcga ggaaaccatc gcgcgggatt accagtcgcc tgccgattca tcccggggcg
     6421 acgtgcctcc ggacctcatt gccgcctacg gcgcggcgca cgacgaatac gagcacatgg
     6481 gcggatacga gtacgacgcc cgctgtgcgt cggttctctc gcgcatgggc ttccgcgcgg
     6541 aggattatga ccagcctgtg gagctgctga gcggcggcca gcagagccgg ctcacccttg
     6601 cggcgctcct cgtgcgccaa cccgacgtac tcgtgctgga tgagcccaca aaccacctcg
     6661 acatcgaagc gacggagtgg ctggaaggat ttctgtcgtc ctacaaaggc gcggtgatcg
     6721 tgatttccca tgaccgggtg ttcctcgatc gtgtcgttac ggaaatcgtc gaaatcgaac
     6781 gcatgcggct cgtccactac cggggcaact acagcgcata cgccgctcaa aaagaggaga
     6841 ggcggcaaca ggagacgaaa gcctacgaat tgcagcgcga gatgatcgag cgcacggaag
     6901 aattcatccg ccgcaacatt gccggacaga aaaccaaaca ggcgcagggg cgacggaagc
     6961 aactggagaa actggagcgt ctcaccccgc cgccgttcca gcggtcggtg agcctttcgt
     7021 ttgtggcgag ccatcgcggt ggaaatgagg tgatcgaggt ccgttcgctg agcaaggcgt
     7081 acaatggcct tccgctgttc gaagagcttt cgtttctcgt gcgcaggggc gagcgggttg
     7141 gcgtggtcgg gccgaacggg tccggaaaat ccaccctcct gcgcatgctg gtcggtgagg
     7201 aagagccgga cggcggtatc atacgcttcg gcgtcggcgt ggaggcggga tactatgacc
     7261 agcgccgcga aggccttgat caggaaagcc gtgtcattga cgaggtctgg agcgtgaaac
     7321 cgagcctcac gacggaacag atccggtcgt accttggttc atttctgttc accgaggagg
     7381 agcagttccg catcatcggc acgctttcgg gaggggagca gagtcgggtc gcgctggcga
     7441 aactcgttct ggcgcaggtc aacctgctga tcctggacga acctaccaac cacctcgata
     7501 ttcccgcgcg ccttgcgctg gagaatgcgc tcgaccgcta cgatgggaca ttgcttgtgg
     7561 tttcgcacga ccgcgcgttc ctcagacgac tgacgacgcg agtgatccgg attcaagacg
     7621 gttctgcaca ggtgtttgac atgggctatc gcgcgcttga ggcggctcag gccgaagcaa
     7681 gggagaagga ggcgcgggaa gcggttgctc gtggtttggc aaccgatgag gcggcaccga
     7741 gacaggttgt cgcggagact tctcccgcgg ataaggcggc ccggcttgcc gcctacgagc
     7801 gaaaacgcga actggagcgc aggcgaaggc aacgtgaacg ccgtttcgcc gacatcgagg
     7861 cggagatcat ggatgtggaa aaccgcctga tggaaatcga cgatgaactc ggcaatcctg
     7921 cccacgccgc tgactgggag atgcttcgca ccctcaccgc tgaacgcggt ctcgccaagg
     7981 agaagctcga ttcgctctac gagcagtggg cggagatgga gcgccacagc tccgcaggat
     8041 tggactgaac ggtcgcggtt gagcctggaa tgcgctccgg ctgcgcagtt caaaccattt
     8101 cgttgacgca ggaaatttac ccgcgtatac tcttcgtcaa caattgtctc cccggggaag
     8161 gagcttcgtt tcgtgaaata ccgatttgtt gcggtaatcg agaaggatga ggacggttac
     8221 tacgccttct gtcccgaact ccagggctgt tacacgagcg gcgacacgtt tgaagaggca
     8281 cgcgccaacc ttgcggacgc aataagactg cacctcgccg accggctggc aaccggagaa
     8341 accttcagaa cgcccgagag catcaacatg aacactctcg agattgcggt atgagtgacc
     8401 gcctgccaag gattacggcc aaggacatca ttcgggttct tggacggcat ggcttttctc
     8461 tgacccggtc cagtggtagt caccagattt tcaagaacca ggcaggtgcg cgggtgacgg
     8521 ttccagtcca cacggaaaaa gccttacacc cgaaagtgct ttagagcatc ctccgcgaca
     8581 tgggtatgac ggccgacgaa ttaagggcgg aactcggccg ttgagcgggg aacacagaca
     8641 gaagtccctc aaaaagagcc ctgcgcgcgg gcggccttac caacgcgaaa tctgatccga
     8701 tttgtacttt gcggtcgggt gtggagcgcc acagctccgc gggattggac tgaacgatcg
     8761 cggggacgtg ccggaggaag gagtgttcgt cttccgccag gacatagttc cgagcacgcg
     8821 aagggaggtg agcatggccc gtatttccga catcatccgc ttcatcgaaa cgcgttccgg
     8881 gcacccgctg aacgggg
//
LOCUS       DAOTNG010000115         8891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7164_length_8891_cov_4.876867, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000115 DAOTNG010000000
VERSION     DAOTNG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_8891_cov_4.876867"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..171
                     /locus_tag="P5179_06990"
     CDS             1..171
                     /locus_tag="P5179_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS94998.1"
                     /translation="MGVCPVDAISLFKAELLIDHAKCISCNFCVDVCPSRSLHGEEEK
                     DTRAENRPGVSP"
     gene            168..1331
                     /locus_tag="P5179_06995"
     CDS             168..1331
                     /locus_tag="P5179_06995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRS94999.1"
                     /translation="MKMRDSYDIVVVGGGPAGCEAALFAAQNGASVLVVEKDPDIGIP
                     VRCAEGISVRALREFYEPDPLFCRQHVNEYQLVAPNGTRVEVSGIGEGVILDRKVFDR
                     MVAEDAARAGAHIMVNASAVGASREGDGVVVHLEGLPDVRCRVLIGADGVESRAGRWL
                     GLRTACRPHDMETAAQFVLGGIDIVPTRFEMHFGTQLAPGGYLWVFPKGKGVANVGLG
                     ISGSHGGSATPLELLERAVQRLFPGASVLGRVLGGIPCSGGLKTIVGDNVMLVGDAAH
                     QANPLTGGGIATGLKAGRIAGRVAAEAIRAGDCSRRALQLYEDEWNDRLGNLHRRFYR
                     IKEAVFNIPDETLNDLADVVNKVPLEERTMNKILARSLAKKPRILLDLARVMF"
     gene            complement(1439..2713)
                     /locus_tag="P5179_07000"
     CDS             complement(1439..2713)
                     /locus_tag="P5179_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007634861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95000.1"
                     /translation="MPQMPEKTPGKTDWFIHDRFGMFIHWGLYALPARHEWVKSREMI
                     PDEKYDVYFRHFDPDLYDPTVWAKAAKNAGMKYFVVTTKHHEGFCLWDSDLTDYKATN
                     TPYGKDLLKPMVEAFRAEGLKVGFYHSLIDWHHPEFPVDCMHPMRNDKEFRERTKGRD
                     ITKYAEYLHGQTRELLTRFGKIDIMWFDFSYPTQPDGGKGRNDWQSEKLYELVRKLMP
                     DVVLDDRLDLEKGYDIKTPEQWQPQEWVKVDGKPVVWEACQTLNGSWGYDRDNLDWKS
                     PALLLWMLVDSVSKGGNLLLNVGPTGRGEFDERALATLQAIGKWTRVNGRSIYGCTAS
                     DYTPPPDCRYTQNGNRLYLHVMAWPLRHLHLKGIADRIEYAQFLHDASEIKIFQNTFD
                     HSIETDNTVVLEIPIQKPNVEIPVIEIFLKDR"
     gene            2869..4779
                     /locus_tag="P5179_07005"
     CDS             2869..4779
                     /locus_tag="P5179_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95001.1"
                     /translation="MPEYDSVCRIAYERYAVEIRPNNFTLALRDLALGAGWISGKPFV
                     RLRIPRRTGKTPVYWGSPEPYELVEWDSSCFSAEAEGRRLGVALEGLQTCYGRVGVEC
                     AIIIERDRVRFEVERVTGLPAGAEIIVDFPYRLGFVQAPGKEPVPGGGLILPRGAGIM
                     VDFSQREGAFPLENLIYSGGQNGYSMPLFGICDGVTTLGTIVKTPYDCYLVGEINSGD
                     PPAYAVSPGFLFEAGRLDYSRRADYRAFEGGYGELASWYRSDLQAEKRYRTLEEKAEG
                     HPLVHTLPGSVLAEMALDFNGTGGGRTPEELADTAKRMGFPGLVSYATNIWQRPFFGT
                     NAVPPNRGTEEDLARGAQYARSVDKAYYISVYENLIDMWPMTPGYDLAIMAKLRDGSP
                     RPNWYAEHLKMRSSTVCSVCRLETSRRDHPHLKELIGEGSIYIDVEGAMELNECFDSL
                     HPLTREQDAGMRRELLSHVRDLFGTVATESMPMDFLADIVDVGAYFPVYQFIGYGCSD
                     KPLLIPPAIPIPLFVMVYHGSVLNMTPKSTDFYTCDPLYVPLWGLMPDETDEFSLRIS
                     KTMRETSYAALVEHRFLAPPRVDTEKGFHSADVQFSRFSDGTCVLANFSGEPYSWMGK
                     EAQPGDYFLWRE"
     gene            complement(4781..5851)
                     /locus_tag="P5179_07010"
     CDS             complement(4781..5851)
                     /locus_tag="P5179_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS95002.1"
                     /translation="MLTRIDRYVVNRFSRTVAFALAAFLVVYVVVDLIDHLDDFFSRE
                     VALSDIVRYYGNYAPYILVLTLPVAVLLATLFVIGDMGGHNELVAISAAGISVYRVAL
                     PLLRLGLLLSLAALLLTELVVPGANDERSRILQLAPQGILSGHAFHVCRQDRSGLILY
                     AERYNIAAKRAQRVAIVQLRGGVPVRRIDASEMVWDDLGWQLQSALDRSFLGDSTVFA
                     SREVMRLPLLTIQPEDLARVDKLPEQMSFLELRDYIRRTRLVGGDASRWLVDLHLKFA
                     FPFANLMMVWIGFPLAARSWRGGRAAYVGLTFLVGFLYFVVVRSGQALGRSGDLHPVV
                     AAWGANALFFVIGAALFRWTRK"
     gene            complement(5874..7145)
                     /locus_tag="P5179_07015"
     CDS             complement(5874..7145)
                     /locus_tag="P5179_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS95003.1"
                     /translation="MSYSLSPFSPVRSGAHRRPGAWVRLRWLRPGLFALGAIVVAAFV
                     LYTLLVVRTLRQDAQRVAKLYAEKILPRAYSDPTITPGELRLLFDLIREMPVAVIVTD
                     DTGNPVSWKGIDVPDTARSPQAMARIRRVVEDMDRSMPPRKVLVPLPDNRSLQMEIHV
                     AESAFLRRIAWMPVVAAIVTLVFAGIAFWGFVQIKSGEQQALWAGMTKETAHQLGTPI
                     SSLSGWLEILKEEAEAAGARRRRADGVILDMSHDVERLRRIAQRFGQIGSQPELRVEP
                     LIPVIDETLDYFRVRLPTLGRDVQIERNYRAGEEIPLNRELLGWAFENIIRNSLDALA
                     RVEGTALIRVTTDRMDNHVRILFEDNGKGIPAGDLPKVFLPGFSTKKRGWGLGLTFVK
                     RIVEDYHGGTITARSGGAEQGTTIEIRLPLD"
     gene            complement(7142..8533)
                     /locus_tag="P5179_07020"
     CDS             complement(7142..8533)
                     /locus_tag="P5179_07020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS95004.1"
                     /translation="MRRWQDITITLISDVVSSLVAFAVALAVRNASASDYLPFPLSAY
                     VVPALLLSLAWAGLFAYVGLYYDWSRKGRFDELVLLTKAVLFGGVVIFLLTFDPNSPL
                     PPTRVVLLTYGLTLVVVSGTGRFAIRGLQRRLFLRGIGLRNALVVGTGQSAVQFAETL
                     KRTPRLGLRVAGFLATETVTNPSPLAQPILGRLNELGPVIEQHQVTDVIFAEPSLSHK
                     TVLDAVALCSGRKVNFGSLPDLYDVVGRTGAGGQLYGVPLLPLYPSDMPVWQRRTKRV
                     LDFLVAFFALVAGLPLWLLVALAVWLEDKGPVFYSQDRVGKDGRVFRFHKFRSMIPNA
                     EKATGPVWAQKNDPRITTVGSIIRKLRIDEVPQLWNVLRGEMSLVGPRPERPYFVERL
                     AAEIPLYRRRLLVTPGITGWAQTKHAYDQTLDDVREKLKYDLYYIENMSLRFDFLIIL
                     RTIWVMLTGKGAQ"
     gene            complement(8586..>8891)
                     /locus_tag="P5179_07025"
     CDS             complement(8586..>8891)
                     /locus_tag="P5179_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRS95005.1"
                     /translation="LRESVNLSLDAVPEGINLREIESYLRNLDQVKDIHDLHVWALST
                     TETAMTVHVVTTSEALDNGVVADIQTYLHEHYGIRHATIQLESDDGSLKTLNCRECN"
BASE COUNT         1823 a         2516 c         2711 g         1841 t
ORIGIN      
        1 gtgggcgtat gccccgttga cgccatctcc ctttttaaag cggagttgtt gatcgaccac
       61 gcgaagtgca tttcctgcaa cttctgcgtc gacgtgtgtc cttcccgttc actgcacggt
      121 gaggaggaaa aggatacacg ggctgagaac cggccgggag tctcgccatg aagatgcgcg
      181 actcatacga catcgttgtg gtgggtggtg gccccgcggg atgcgaggcc gcactgtttg
      241 cggcgcagaa cggtgcgtcg gtgcttgtgg tggagaagga tccggacatc ggaattcccg
      301 tccggtgcgc ggaagggatc agcgtcaggg cacttcgcga attctacgaa ccggaccctc
      361 tgttctgccg acaacatgtg aatgaatacc agctggttgc cccgaacgga acacgggtag
      421 aagtgagcgg gattggagaa ggcgtgatcc tcgaccggaa ggtatttgac cggatggtgg
      481 cggaggacgc ggcgagggcg ggtgcccaca tcatggtgaa tgcgagcgcc gtcggggctt
      541 ccagggaggg tgacggagtt gtcgttcatc tggagggttt gccggatgtg cgctgccggg
      601 tgttgatagg cgctgatggt gtggaaagcc gggcaggccg ttggcttggc ctgcggactg
      661 cgtgtcgtcc ccatgacatg gaaacggctg cccagtttgt gctgggtggg attgatatcg
      721 ttccgacgcg attcgagatg catttcggaa cacaactcgc cccgggcggt tacctctggg
      781 tgtttccgaa agggaaaggc gtcgcgaatg tcggtctggg aatcagcggc tctcacggcg
      841 ggtctgccac tccgttggag ttgctggagc gcgcggttca gaggcttttt cccggtgctt
      901 cagttctcgg gcgcgtgctg ggtgggatcc cgtgctcggg cggcttgaaa acgattgtag
      961 gcgacaacgt gatgctggta ggagatgcag cccaccaggc aaatccgctc accggtggcg
     1021 gcatcgcgac gggcctcaaa gcgggaagga ttgcggggag agtggctgcg gaagcgattc
     1081 gggcgggtga ctgctcgcgg cgggcgttgc aattgtacga ggatgagtgg aacgaccggc
     1141 tcgggaacct gcacaggagg ttttaccgaa tcaaggaggc agtattcaac attcccgacg
     1201 aaaccctgaa cgatctggct gacgtggtga acaaagttcc gctggaagag cggaccatga
     1261 acaaaatcct cgcgcgatcg ctcgcgaaaa agccccgaat cctcctcgat ctggcccggg
     1321 taatgttctg aacctcgacc ggatttcggg acgggcacac tccttgccgt aaattccccc
     1381 gaaaaaaagg ggacgcaact cgcaagctgc gtccccttcg ccagagtgct tcacgccatt
     1441 atcggtcttt gaggaagatt tctattaccg ggatttccac gttcggtttc tggatgggga
     1501 tttccagaac taccgtgttg tccgtttcga tggagtggtc gaacgtgttc tggaaaatct
     1561 tgatctcgct cgcatcgtgc aggaactgcg cgtactcgat tctgtcagca atccccttga
     1621 gatgcagatg ccgcagaggc cacgccatca cgtggagata aagccggttg ccgttctgcg
     1681 tgtagcggca atctggtggc ggcgtgtaat cgcttgcggt gcaaccgtat attgatcgac
     1741 catttacccg cgtccacttc ccgattgcct gcagagtggc aagcgcgcgt tcgtcaaact
     1801 cccccctgcc cgtgggaccc acgttgagga gcaggtttcc tcctttggac acgctgtcaa
     1861 caagcatcca cagcagaagc gccggcgatt tccagtcgag gttgtcccgg tcatatcccc
     1921 agcttccatt cagggtctga caggcctccc acaccacggg ttttccatcg accttcaccc
     1981 actcctgcgg ttgccactgc tccggcgttt tgatgtcata ccctttctcg agatcgagcc
     2041 ggtcgtcaag aacgacgtcg ggcatcagct tccgaacaag ttcgtagagc ttctcggatt
     2101 gccagtcgtt ccgtcccttt cccccgtccg gctgggtggg gtatgagaaa tcgaaccaca
     2161 tgatgtcgat ctttccgaaa cgggtcagca gctcccgggt ctggccgtgg aggtattccg
     2221 catacttggt gatgtcgcgg ccttttgtcc gctcgcggaa ctctttgtcg ttcctcatgg
     2281 ggtgcatgca gtcgacggga aactccgggt ggtgccagtc tatcagcgaa tggtagaaac
     2341 caactttcag cccctccgcg cgaaacgcct ccaccatggg cttcaaaagg tctttcccgt
     2401 agggagtgtt cgttgctttg taatctgtca ggtcggaatc ccacaggcag aaaccttcgt
     2461 ggtgcttggt ggtgacgacg aagtacttca tgccggcatt tttcgccgct ttcgcccata
     2521 cagtcgggtc ataaaggtca gggtcaaaat ggcggaagta cacgtcgtat ttctcgtcag
     2581 gtatcatctc ccgcgatttc acccactcgt gccgcgctgg cagggcatac agtccccagt
     2641 gaatgaacat tccaaacctg tcatggatga accagtcggt ttttccaggt gttttttccg
     2701 gcatctgcgg catgatgtga tctccttgaa gtagaggacg cactacgaag tgacgtcgaa
     2761 aacatgtgaa cgtgcgctga cggcgtcaac ggggaggtgt tgccgaacct gctttggccg
     2821 cctattcttc gggtaccatg tcgaaccaga acaggaaggg agccgaccat gccggaatac
     2881 gattcggttt gccggatagc gtacgagcgg tacgcggtag agattcgtcc gaataatttc
     2941 acgctggcgt tgcgggatct cgctctgggt gccgggtgga tttccgggaa gcctttcgtc
     3001 cgtcttcgca taccccgccg cacggggaaa acacccgttt actggggctc tccagaacca
     3061 tatgaattgg tcgaatggga ttcatcgtgc ttttcggcag aggccgaggg caggcggctt
     3121 ggagtggcgc tggaggggct ccagacgtgt tacgggcgtg ttggagtgga gtgcgcgatc
     3181 atcatcgagc gcgaccgcgt gaggttcgag gtggagaggg tgaccggcct gccggccggt
     3241 gccgagatca tcgtcgattt tccgtaccgc ctcggcttcg ttcaggctcc cggaaaggaa
     3301 ccggttcccg gcgggggact catattgccg cggggcgcag ggataatggt ggatttctca
     3361 cagcgagaag gtgctttccc gctggaaaac ctgatctaca gcggcggcca gaacggctac
     3421 tcgatgccgt tgtttggcat atgcgacggc gttaccaccc tcggaacaat tgtcaaaaca
     3481 ccgtacgatt gctacctggt gggtgagatc aactcgggag atcccccggc atatgcggtt
     3541 tcgccgggct ttctgttcga agcggggcgc ctggactact cacggcgggc agactaccgt
     3601 gcctttgagg ggggctacgg tgagcttgca tcgtggtatc ggtcggattt gcaggctgag
     3661 aagcgttaca ggacgctgga ggagaaagcc gagggtcatc ccctggtcca caccttgccg
     3721 ggctctgtgc ttgcggaaat ggcccttgat ttcaacggaa ccggtggcgg gcgaacgcca
     3781 gaagaacttg ccgacaccgc aaaacggatg ggcttcccgg gtctggtgtc gtacgcaacc
     3841 aacatctggc agcggccgtt cttcggaacg aacgcagtgc cgccgaatcg ggggactgag
     3901 gaggaccttg cgcgaggcgc gcagtacgca cggtcggttg acaaggcgta ttacataagc
     3961 gtgtacgaaa acctcatcga tatgtggccc atgaccccgg ggtacgacct tgcgatcatg
     4021 gccaagttgc gtgacgggtc gccacgcccg aactggtatg cggagcattt gaagatgcgg
     4081 tcgagtactg tctgctcggt gtgccggctg gagacttcac gccgtgatca tccgcacctg
     4141 aaggaactca tcggcgaagg ttcgatctac attgacgtcg agggcgcgat ggagttgaac
     4201 gagtgcttcg attcgttgca tcctcttacg cgagaacaag atgcaggcat gcgtcgagag
     4261 ctgctttcgc atgtccggga tctgttcggc acggttgcga cggaatccat gccgatggat
     4321 ttcctcgcgg atatcgttga cgtcggggcg tattttcccg tgtaccagtt catcggctac
     4381 gggtgcagtg acaaaccgtt attgatccct cctgccattc ctatcccatt gtttgtcatg
     4441 gtgtaccatg gttccgtgct gaacatgaca ccgaagtcca cggatttcta cacctgcgat
     4501 ccgctctacg ttcctctctg gggtttgatg ccggacgaaa ccgacgagtt ctccctacgg
     4561 ataagcaaga cgatgcggga gacaagctac gctgcgctcg ttgagcaccg cttcctggct
     4621 ccacctcgcg tggacacaga aaagggcttc cattcggcgg atgttcagtt ctcaaggttt
     4681 tctgatggaa cgtgcgtgct cgcgaatttc tccggcgagc catactcgtg gatggggaag
     4741 gaagcgcagc cgggggacta ctttctctgg agggagtgag ctacttcctc gtccagcgga
     4801 aaagcgcggc gccgatcaca aagaaaagcg catttgctcc ccatgccgca acaacggggt
     4861 ggagatctcc agatcgtccg agcgcctgcc cggagcgaac tacgacgaag tagaggaacc
     4921 cgacgaggaa cgtgaggccg acatacgctg cgcgtccacc tcgccacgag cgggctgcga
     4981 gagggaagcc gatccagacc atcataaggt tcgcgaaagg gaacgcgaat ttcagatgaa
     5041 ggtccacgag ccatctggaa gcatccccgc cgaccaacct ggtgcgccgg atgtaatcgc
     5101 gaagctcaag gaaggacatc tgttccggaa gcttgtcgac gcgcgccagg tcctccggtt
     5161 ggattgtgag caatggcaac ctcatcacct cccgcgaggc gaacaccgtg ctgtccccca
     5221 gaaaggacct gtccagcgcg ctctgaagtt gccatcccag gtcatcccat accatctccg
     5281 acgcgtcgat ccggcgtacg gggaccccgc ctcgaagctg cacgattgcg acacgttgcg
     5341 cccgtttcgc cgcgatgttg taccgttctg cgtagagaat caggccgctt ctgtcctgcc
     5401 tgcacacgtg gaaggcatgt ccggacagaa ttccctgcgg cgcaagttgg agaattctgc
     5461 tgcgttcgtc atttgcgccg gggacgacaa gctcggtgag caacagcgcc gcgagtgaca
     5521 gaagcaatcc gagcctcagc aacggcaggg caacccggta cacgctgatt ccggcagcac
     5581 ttatggcgac caactcattg tgccccccca tgtcgccgat cacgaaaagc gtggcaagaa
     5641 gaacagcaac gggcagggtg agcacgagga tgtaaggagc ataattcccg tagtaccgca
     5701 caatatctga cagcgcgact tcccgggaaa agaagtcgtc aaggtggtct atgaggtcca
     5761 ccacaacgta cacgacgaga aatgccgcca gcgcgaatgc aaccgttcgt gagaacctgt
     5821 tgacgacgta acggtcgatt cgagtgagca cggaaaaccc ctgtggcggt atgtcagtct
     5881 aatggaaggc gtatttcgat cgttgtcccc tgctcggcgc cccccgaacg agcggtgatg
     5941 gtgcctccgt ggtaatcctc cacgattcgt ttgacgaacg tgagaccaag cccccagccg
     6001 cgtttctttg tgctgaaccc gggaaggaac actttaggga gatccccggc gggtatgccc
     6061 ttcccgttgt cctcgaacag aatccgcacg tggttgtcca tcctgtctgt cgtgacgcgt
     6121 atcagtgcgg tgccttcaac gcgcgcgagg gcgtcaagag agttgcgaat gatgttctca
     6181 aatgcccatc ccagcaattc gcggttgagt ggaatctcct ccccggcccg gtaattgcgc
     6241 tcgatctgca cgtctctccc cagcgttggc aggcgaacac gaaagtaatc aagggtttcg
     6301 tcgataacag gaatcagcgg ctccacacgc aattccggtt gtgatccgat ctgtccgaaa
     6361 cgctgagcaa ttcgacggag gcgttcgaca tcgtgcgaca tgtcgaggat cacgccgtcc
     6421 gcgcgccgtc gtctcgcgcc tgccgcctca gcttcctctt tgaggatttc gagccagccg
     6481 gacaggctcg aaattggtgt gccgagctgg tgggccgtct ccttggtcat ccctgcccag
     6541 agggcctgct gttcaccgga cttgatctgc acgaagcccc agaatgcaat acccgcgaaa
     6601 accagtgtta ctatcgccgc cacgaccggc atccaggcga tgcgacggag gaatgcgctc
     6661 tccgccacgt ggatctccat ctggagggac cggttatccg gcaatggaac caacaccttg
     6721 cgaggcggca tggaccggtc catgtcctca accacccgcc tgatgcgtgc catggcttgc
     6781 ggggagcggg ctgtgtcggg aacatcgatc cctttccacg acacggggtt accggtgtca
     6841 tccgtcacga ttacggcgac cggcatctcc cgaatgaggt cgaacaggag gcgcaattcc
     6901 ccgggcgtaa tggtaggatc gctgtaggct ctgggaagga ttttctcggc gtacagtttt
     6961 gccacgcgtt gggcatcctg tcgcagcgtt ctgacgacca gcagcgtgta caacacgaac
     7021 gctgccacga caatcgcccc gagagcaaaa agccccgggc gcagccatcg cagccttacc
     7081 cacgcgcccg gacggcggtg ggctccggac cgcaccggcg agaacggtga caacgagtag
     7141 ctcactgcgc gcctttgcct gtcagcatca cccagattgt cctgagtatg atcaggaagt
     7201 cgaaacggag cgacatattc tcgatgtaat aaaggtcgta cttcagtttt tcgcggacgt
     7261 cgtcaagggt ctggtcatat gcatgtttcg tctgtgccca tccggtaata cccggcgtaa
     7321 caagaagccg acgtcggtaa agcgggatct ccgctgcgag gcgctcgacg aaataggggc
     7381 gttcgggccg gggaccgaca aggctcattt cgccccggag aacattccac agttgaggca
     7441 cctcgtcaat gcgcagcttg cgaataatcg atccgaccgt cgtgatgcgg ggatcgtttt
     7501 tctgcgccca cacaggcccg gttgccttct ccgcgttcgg tatcatggat cggaatttgt
     7561 ggaaccggaa cacgcgtcca tccttaccga cgcggtcctg ggagtaaaac accgggcctt
     7621 tgtcctccag ccagaccgcc agcgctacca gaagccacaa cggtagaccc gctaccagag
     7681 cgaaaaacgc aacgaggaag tcgaggacac gtttggtcct gcgttgccag accggcatgt
     7741 cggatggata gagagggaga agcggaacgc cgtaaagctg tccccctgcg ccggttctcc
     7801 ccacgacgtc atacaggtcc ggaagactac cgaaattcac tttcctgccg ctgcacaacg
     7861 ccacggcgtc cagcacggtt ttgtggctca gcgaaggctc ggcgaagatg acgtcggtga
     7921 cctgatgttg ttcgatgacg ggcccgagtt cattcagacg gcccaggatt ggctgtgcga
     7981 gcggggacgg gttggtgacc gtttcggtgg cgaggaaccc cgcgacacgc aaccccagtc
     8041 ggggcgttcg tttcagagtc tccgcgaact ggactgctga ttgtccggtt cccactacca
     8101 gagcgtttct gagaccgatt ccgcgcagga acaggcgtcg ttgcagcccg cgtatggcaa
     8161 atcgccctgt gccactgaca acgacgagcg tcagtccgta cgtgagcagc accacgcggg
     8221 ttggtggaag cgggctgttc gggtcaaacg tgagaaggaa tatcacaacc ccaccgaaca
     8281 ggacggcttt cgtgagcaga accagttcgt cgaagcgtcc tttacggctc cagtcgtaat
     8341 acaggcccac gtacgcgaag agaccagccc atgcgaggga gagaagcagt gcgggaacca
     8401 cgtatgcgga gagcgggaag ggaaggtaat cagacgcgga cgcgttgcga acagcaaggg
     8461 cgacagcgaa cgccaccagc gacgacacga catccgaaat cagcgtgatg gttatatctt
     8521 gccagcgacg catgccgtta ccagagttcc caccgggtgg cgcagtgcga aatcgcccat
     8581 gcagatcagt tgcattcccg gcaattgagt gttttcagac taccgtcgtc gctctcgagc
     8641 tgaatggtag cgtggcggat accgtagtgt tcgtgaagat acgtctgaat gtcagctacc
     8701 accccgttgt ccagagcctc ggacgtcgta acgacgtgaa cagtcatcgc cgtttccgtc
     8761 gtgctcaacg cccacacgtg cagatcatgg atgtctttca cctgatcaag gttccgaagg
     8821 tagctctcaa tctcccgcag attgatacct tcggggaccg catcaaggga tagattgacc
     8881 gactcccgca a
//
LOCUS       DAOTNG010000116         8852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7189_length_8852_cov_5.519382, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000116 DAOTNG010000000
VERSION     DAOTNG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7189_length_8852_cov_5.519382"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="P5179_07030"
     CDS             <1..889
                     /locus_tag="P5179_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95006.1"
                     /translation="RNDLDRRMSELALSVSAFFRVAAQDSFVVSGTLALNKDRTPAET
                     ELNDRDDYRRSLVGAYWHTFGRSTVLELRGERTETHSVWLKTQRSSGNRWDRVLNLWA
                     ITNFERGRLGLSQRTFFRTAMEEFDYDYLTPTNPQSRNTRLGRLEWQAKTRVGERDSV
                     GISYSVEARTLGNLLPSGPGQRPKLWQLTRNELIHVVSVSGVVRAGSSWKIAPSVAYD
                     RQQGYVPTQAQWNPFALGRRVDKQEGFHAEAAISCAPPNGLFSRRDEITASVSRSYRK
                     RAGLTDTMNYISVIYRLHP"
     gene            961..2724
                     /gene="recJ"
                     /locus_tag="P5179_07035"
     CDS             961..2724
                     /gene="recJ"
                     /locus_tag="P5179_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRS95007.1"
                     /translation="MSGSRDPTWVFPPPCAEEEVRSVATRWDIPLTVAEILLRRVNNP
                     DSPAARDDAIARFLNPSFRHLHDPYLYNGMHRAVERICRAVVNREQVSVYGDYDVDGT
                     TATALMVEALQTLGVPVEWRIPHRTADGYGLSDRGVELLRDTAGTLVIVLDCGVTAVE
                     QIATLTREGRDVIVVDHHEEGETLPNALAVLDAKCARCTYPFPGLSAVGVAFKLLQAV
                     CDALRVPPGRILIPGLDLVAVGTAADIVPVIDENRVLMRFGLRRLRKSPRPGLRALLQ
                     VAGLAGKELTTSSIVFGLAPRINAAGRMGSAETSVRLLLTQDEDEARSLARELNQLNR
                     DRQGRDQDVFDSAHRQAEEQVAQGARALVLADERWHPGIIGIVAARLVEEFYLPTVMI
                     TGSTPFARGSGRSIDGFDLHAALGACANTLIGYGGHIKAAGLSIDPQRIPEFRQCLQQ
                     VAAERLTDDLLSPRLYLDGEARLSDITPEYLKAVARLGPFGPDNMQPVLVSRHVRAIS
                     DPVIMKDAHLKVEVEQEGVTREIIGYGFKWAAPLFYDAANRSVSAVDIAYVPEENVWR
                     GKSKTQLRLKAVRRSDEHGRS"
     gene            complement(2721..3191)
                     /locus_tag="P5179_07040"
     CDS             complement(2721..3191)
                     /locus_tag="P5179_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRS95008.1"
                     /translation="MKLRIVVVGKPRRDVVEAFVADYLARLKPIWQTELVAVAEERIS
                     AGRADGEILVKEAERLRNKVPAGWVTVALDRTGRTLDSREFAEALRSWQDSSVRGVAF
                     VIGGAVGLDPAFARGCALRLSLGPMTFPHDLALVVLTEQIYRAATIVKGLPYHR"
     gene            complement(3193..3897)
                     /locus_tag="P5179_07045"
     CDS             complement(3193..3897)
                     /locus_tag="P5179_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS95009.1"
                     /translation="MATQPFSLDAIRSVPLFTNLSDEMLEAVVKASTVVTFRRNQTIV
                     RENEEGNALFIILSGRVKVTLIRSDGKEAILAILRANDFFGEMALLDDQPRSASVVAL
                     ESTRALMLTRPNFIKLLEDDPQIVHNIMVALSGRLRKANQKIADLAFLDAIGRICGVI
                     LQMAEEAGEETDEGILIHNRPSHEQLGMMAGTTRETATHCMIALERRGYLLTQGRDLL
                     LFSPEDLKRDFLSPSS"
     gene            complement(3990..4064)
                     /locus_tag="P5179_07050"
     tRNA            complement(3990..4064)
                     /locus_tag="P5179_07050"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4029..4031),aa:Glu,seq:ttc)
     gene            4133..5326
                     /gene="obgE"
                     /locus_tag="P5179_07055"
     CDS             4133..5326
                     /gene="obgE"
                     /locus_tag="P5179_07055"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRS95010.1"
                     /translation="MAVSGATRAQVRGLEGFFPGAGSMKRSAHSIGLLIESWGAAVAQ
                     TTAGGGNMEFVDTATIEVWGGDGGPGAVSFRHEKNVPRGGPDGGDGGNGGSVLFVGDP
                     RMNTLQEFRFRRHFRARHGERGLGSRWHGASGEDVTIRVPCGTLVFDAETGALIQDIV
                     APHEPVIVAKGGRGGKGNAQFATPTRQAPDFAQNGTPGEYRSLRLELKLLADVGLVGL
                     PNAGKSTLLSVISAARPKIADYPFTTLVPNLGIVSLAEHLTCVMADIPGLIEGAHLGK
                     GLGDQFLKHIERTRVLVFMVESIDPDPDATLKLLRNELREFNPDLLKKPWVYVLSKAD
                     LSDAKPRKARGAHACFSLSAVTHVGLDRLRHELSVLLTETTDAGTDSDSRVRFATRLP
                     ESPPQ"
     gene            5330..6478
                     /gene="dprA"
                     /locus_tag="P5179_07060"
     CDS             5330..6478
                     /gene="dprA"
                     /locus_tag="P5179_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRS95011.1"
                     /translation="MSDRSFEELGDLLALCNVKGIGAARLAALVEAFGSPGAVLAANP
                     SELETVQGIPSAIALAIADMGRGRTDTVRDAVMRQCERIVRTNTSVIACIDADYPPHL
                     LETEDPPQLLFAQGDTGLLAIPGVAVVGTRRPSEYGRHITEAIAATLVRHGVTVISGM
                     ARGVDSIAHTVALDRDGLTVAALGCGVDVVYPPEARNLHARIREHGLLVSEVWLGAAP
                     DRINFPRRNRIISGLSRAVIVTEAPRASGALITADVALRQGRDVYAVPGDITRREAAG
                     ANELIADGARVVRDMDDLVISLGLGTRVPTPEGDQLGLPVSAPADLSPEERVVLDALA
                     LEPVHVDALAVKLGRKSEELLTSLTLMEMRGLAVAHAGSRYSRATMTS"
     gene            6491..8044
                     /locus_tag="P5179_07065"
     CDS             6491..8044
                     /locus_tag="P5179_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS95012.1"
                     /translation="MRLTIGGKVLLGNLGMVALMCAAVLWVVRDLRRMDDLVELTVNA
                     QEQIIRINRISDDINEILYRGGIKVSMFQVGAFAASRDADEMTRSLRSRRAHRDSLLA
                     EVSRSRFGQRPALKYSLDSLLIACRKLDLLFESVELHFERGQPGRAERAFSQLHEVDA
                     ELSRAMTSLQEHAQEDANVLLAAASRFREHLILRAGVVLAVLLAGMLAMVMYLNRTIK
                     RATAGISNALSAVASGDFQQRVELVSKDEFSEIAEGLNATVERLGELDALKADFLSKV
                     SHDLRTPIAAVKQAAELLNDGIPSPLKEDQKEILQIIRTNAKRLGNLINDLLDTARLE
                     AGKLEIQPETTDLVAIIRRVATSIAPLSMEKRLRITLKAAPDLPPAYADPNRMEQVLM
                     NLLGNAVKFTPENGEIALSCEQEGAFLRCSVRDTGVGIPPEDLPRIFDKFHQVRMKRA
                     TKAKGTGLGLTIVKFLVEAHGGFVWAESTPGQGATFTFTVPVVEQPGEGEDVRYSGTR
                     EEDADVRPD"
     gene            8188..8790
                     /locus_tag="P5179_07070"
     CDS             8188..8790
                     /locus_tag="P5179_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS95013.1"
                     /translation="MEQAPAVAFLLQMPELFLVTGERNRTTGMHEAATARFAGPVTAA
                     EVGGNMEPLRVFVVDDDEAFVQLVTAVLSAKFGHWVASETDAVKAVERIKAEQPDVVL
                     LDLVMPRQDGYETLKFLLADEKTRDIPVVMLTNFDADKFRSMAERWGASAFLGKKAIG
                     LSAFLNRGAQADDLPFAQKDDLNYAQLDEVLRKVVTNRTA"
BASE COUNT         1755 a         2594 c         2821 g         1682 t
ORIGIN      
        1 gcgcaatgac cttgatcgcc ggatgtcaga acttgctctc tcggtgtcgg cgttctttcg
       61 cgttgccgcc caggattcat tcgtggtcag cggcacgctg gcgctgaaca aggaccgtac
      121 cccggcggaa acggaattga acgatcggga cgattaccgc cggtctctcg tcggcgccta
      181 ctggcataca ttcgggcgaa gtacggtact cgagctgcgc ggcgaacgaa ccgaaaccca
      241 cagcgtgtgg ctgaaaacac agcgttcttc cgggaaccgg tgggaccggg tgctcaacct
      301 gtgggccatc acgaatttcg aaagagggcg gctcggcctc tcgcaacgca ccttcttccg
      361 gaccgcgatg gaagaattcg actatgatta cctgaccccc acgaatccgc aaagccggaa
      421 caccaggctt ggacggctgg agtggcaggc gaaaacccgg gtcggtgagc gcgactcggt
      481 cgggatttcg tattcggtgg aagcgcggac gctgggaaac ctgctgccct ccggacccgg
      541 ccagcgccca aaactatggc agctcacacg gaacgaactg atccacgttg tcagcgtgtc
      601 cggggttgtt cgggccgggt cgtcatggaa gatcgcaccc tccgttgcgt acgaccgcca
      661 acagggatac gtcccgactc aggcgcaatg gaacccgttt gccctcggca ggcgcgtgga
      721 taagcaggag gggttccacg cggaggctgc gatctcgtgt gcaccgccga acgggttgtt
      781 ctcaaggcgg gacgagatta ccgccagtgt ttcccgatcc tatcggaagc gtgctgggct
      841 gaccgataca atgaactata tttctgttat ctatcgcctc catccctgaa cacaccggcg
      901 gcatccgaag gtttcacggt gcggttttta acgcagtagc gcaccccgga gcatgtactg
      961 atgagcggat cacgcgatcc cacgtgggtt tttcctcctc cgtgcgcgga agaggaagta
     1021 cgcagtgttg caacgcggtg ggacatcccg ctcacggttg ccgagatcct gctgcgccgg
     1081 gtaaataatc cagattcccc cgccgccaga gacgacgcaa tcgcacgctt cctgaacccg
     1141 agcttccgac atctccacga tccgtacctg tacaacggga tgcaccgggc ggtcgagcgg
     1201 atctgccgtg cggtggtgaa ccgggaacag gtaagcgtgt acggcgacta cgacgtggat
     1261 ggcacgacag ccaccgcgct catggtcgaa gcgctccaga ccctcggtgt tccggttgag
     1321 tggcgcatcc cgcaccgaac cgcagatggc tacggcctga gtgatcgcgg agtggagttg
     1381 ctcagggata ccgcgggcac gctggtgatc gtactcgatt gcggcgtgac ggcggtggag
     1441 cagatcgcca cactcacgcg ggagggtagg gacgtcatcg tcgtggacca ccacgaagag
     1501 ggcgagacgc tcccgaacgc gctggccgtt cttgacgcga aatgcgcgcg ctgtacctac
     1561 ccctttcccg ggttgtccgc agttggcgtg gcgttcaaac tgcttcaggc tgtgtgcgac
     1621 gcgttgcgcg ttccccccgg caggattctg attccagggc tcgatctggt ggccgtagga
     1681 accgcggccg acattgtgcc tgtgatagat gagaaccgcg tgctgatgag attcgggctc
     1741 cgcaggctga ggaaatcacc ccgcccgggg ctgcgggcgt tgctgcaggt tgccgggctg
     1801 gcggggaaag aactcaccac gtccagcatc gtattcggtc ttgcgccgag aatcaatgcg
     1861 gccggacgga tgggcagcgc ggaaacgtcc gttcgactgc ttcttacgca ggatgaggac
     1921 gaggcaagaa gcctcgcgcg tgaactgaac cagttgaacc gggaccggca ggggcgcgat
     1981 caggacgtgt tcgacagcgc acaccggcag gcggaagagc aggttgcgca gggtgccaga
     2041 gcgctggtgc ttgcggacga gcgctggcac ccgggtatca tcggaattgt ggccgcacgt
     2101 ctcgtggaag agttttacct tcctaccgtc atgattaccg gctccacacc cttcgcgcgg
     2161 ggttccggca ggagcataga cgggtttgat cttcacgccg cccttggcgc gtgcgcgaat
     2221 acgctgatcg gctacggggg gcacatcaaa gcggcgggcc tcagtattga ccctcagcgt
     2281 attccagagt tccgacagtg tctgcagcag gtggcggcgg aacgcctgac ggacgacctt
     2341 ctctccccgc gtctgtacct cgacggtgaa gcacggctgt ccgacatcac gccggaatac
     2401 ctgaaagcgg tcgcccgtct cgggcctttc gggccggaca acatgcagcc ggtgctggtt
     2461 tcgcgccatg tacgcgcgat atccgacccg gtcatcatga aggatgccca tctgaaggtg
     2521 gaagttgagc aggagggtgt tacgagggag ataatcggct acggtttcaa atgggctgcg
     2581 ccgctcttct acgatgccgc gaaccgttca gtaagcgcgg ttgacatcgc ctatgtgccg
     2641 gaagaaaatg tgtggcgcgg gaagtcgaaa acgcagctcc gcctgaaagc cgtccgaagg
     2701 agcgacgagc atggccgcag ttagcgatgg tagggaagtc ccttgacaat cgtcgccgcg
     2761 cggtaaatct gctcggtcag aaccaccagc gcgaggtcgt gcgggaaggt catcggccca
     2821 agcgataggc ggagcgcgca tccgcgtgca aatgccgggt ccagccccac tgcaccaccg
     2881 atcacgaacg ccacgccgcg caccgaagaa tcctgccacg agcgcagcgc ctctgcgaac
     2941 tcccgtgagt ccagcgtcct gccggtacga tctaatgcaa ccgtaaccca gcccgcgggg
     3001 accttgttcc gtagtcgttc cgcttctttt acgaggattt ccccgtcggc acggcctgct
     3061 gagatccgtt cctcagccac cgcgacgagt tccgtctgcc agatcggttt caaacgggcg
     3121 agatagtcgg caacgaacgc ttcgacgaca tcacgtcgcg gtttgcccac gaccacaata
     3181 cggagcttca cgttacgaac tgggggaaag aaaatcgcgc ttgaggtcct ccggcgaaaa
     3241 caacagcagg tcgcgaccct gtgtcagaag gtacccccgc cgttcaagcg caatcatgca
     3301 gtgtgtggca gtttcccgcg tggtgccggc catcattccg agttgctcgt gggaggggcg
     3361 gttatggatc aggatacctt cgtcggtttc ttcgcccgct tcctccgcca tctgcaggat
     3421 taccccgcag atgcggccga tcgcgtccag gaatgcgagg tcggcgattt tctggtttgc
     3481 cttgcgaagg cgcccgctca acgcaaccat gatgttgtgg acaatctgcg ggtcatcctc
     3541 aagcaatttg atgaaattcg gcctcgtaag catgagcgcg cgggtgcttt cgagcgccac
     3601 cacggatgcc gacctcggtt gatcgtccag gagtgccatt tcgccgaaga aatcattcgc
     3661 gcgcagaatc gccagaatcg cttctttgcc atctgagcgg atcagcgtga ctttcaccct
     3721 gccgctcaaa atgatgaaaa gcgcgttccc ttcctcattc tctcggacaa tggtctggtt
     3781 ccgccggaat gttaccaccg tggaagcctt caccacggct tcgagcattt cgtcggaaag
     3841 attggtaaag agagggacgg accgaatggc gtccagcgaa aagggctgcg ttgccattgt
     3901 ggtactcctc agagggaagt gccggacggc aggatcctga atgctttccc cgaagcggac
     3961 aggtccctcg cccggtctct gaaagcacct ggtgacccgt aggggagtcg aacccctgtt
     4021 gcagggatga aaaccctgag tcctaaccac tagacgaacg ggccacagtg ttcaaagaac
     4081 taataagtat acagaaaaca ccggcgtgat ttcaagaccc gggacaccgg ccgtggcagt
     4141 ttcgggtgcg acgcgtgcgc aggtgcgtgg acttgagggc tttttccccg gtgccgggtc
     4201 tatgaaaagg tccgcccatt ccataggttt gttaattgaa agctggggcg cggcagtcgc
     4261 tcagacaact gccggaggcg ggaacatgga attcgtagat acggcaacta tcgaggtatg
     4321 gggcggagac ggcgggcctg gagccgtcag tttccgacac gagaagaacg taccccgcgg
     4381 cgggccggat ggtggagacg gcggcaacgg aggcagtgtc ctctttgtcg gagatccgcg
     4441 gatgaatacc ttgcaggagt tccgcttccg gaggcatttc cgtgccaggc acggagaacg
     4501 agggctcgga tcacgctggc acggcgcatc aggagaggat gtgactattc gcgtgccgtg
     4561 cgggacgctt gtatttgacg ccgaaacggg cgcattgatt caggacatcg tcgccccaca
     4621 cgaaccggtg atcgtggcca aaggagggcg tggggggaaa gggaatgccc agttcgccac
     4681 gccgacccgg caggcgcctg atttcgccca gaacggaacg ccgggagagt accgttctct
     4741 caggcttgag ctcaaattgc tggccgatgt aggtcttgtg ggtctgccga atgccgggaa
     4801 atcaaccctc ctgtcggtca tttcggctgc ccggccaaaa atcgcggact atcctttcac
     4861 cacgcttgtt ccgaacctcg gcattgtgtc gctggcggag cacctgacct gcgtgatggc
     4921 ggatatcccc ggtctcatcg aaggtgcgca tctgggtaag ggcctgggtg accagttcct
     4981 gaagcatatc gagcgcaccc gggtgcttgt gttcatggtg gagagcatcg atcccgatcc
     5041 ggacgcgacg ctgaaacttc ttcgcaacga gctgcgcgag ttcaaccccg acctgttgaa
     5101 aaagccgtgg gtatacgtgt tgagcaaagc agacctcagc gatgcaaagc cgcgaaaagc
     5161 tcgtggcgcg cacgcctgtt tctctctgag tgccgtcact catgtcggcc ttgaccgact
     5221 gcggcacgag ctctctgttc ttcttaccga aacgacggac gcgggaactg attccgattc
     5281 acgggtgcgt ttcgccacgc ggctccccga aagtccgcct caataagcca tgtccgaccg
     5341 ttccttcgaa gaactgggcg acctgctcgc gctgtgtaac gtcaaaggta tcggcgccgc
     5401 gcgtctggcc gctctcgtgg aagcattcgg ttcgcctggt gcagtcctgg cagccaaccc
     5461 ttcggagctg gaaacggtcc agggtatccc ttccgcaatc gctctggcaa tcgctgacat
     5521 gggtcgcggc aggactgata cggtgcgcga tgcagtcatg cgccagtgcg agcgcatcgt
     5581 ccgcacaaac acttccgtca tcgcgtgcat cgacgcagac tacccgcctc accttctgga
     5641 aaccgaggac ccgccccaac tcctctttgc gcaaggagac acggggctcc tcgcgattcc
     5701 gggcgtagcg gtggtcggca cgcggcggcc atcggagtac ggccgccata tcaccgaagc
     5761 gattgcggcg acgcttgttc gtcacggggt gacggtgatc agcggcatgg cacgcggggt
     5821 ggacagtatt gcccacacgg tggctctcga cagagacggc cttactgttg ccgcgctggg
     5881 atgcggggta gatgtggtat acccccctga agcgcggaac ctccatgcac gcattcgcga
     5941 acacggtctt ctggtcagtg aagtctggct cggtgcggcg ccggaccgaa tcaactttcc
     6001 tcgccgcaac cggatcatca gcgggctttc gcgtgcggtg atcgttaccg aggcgccacg
     6061 ggcctcaggc gcgctcatca cggccgatgt cgcgctcagg caggggcgtg acgtgtatgc
     6121 cgttccggga gatatcacgc ggcgggaagc ggcaggtgca aacgaattga tcgcggatgg
     6181 ggcgcgtgtc gtccgtgaca tggatgatct ggtaatcagt ctggggctcg gtacccgtgt
     6241 tcccacgccg gagggggatc aacttggtct cccggtatcg gctccggcgg atctctcccc
     6301 cgaggagcgg gtggttctcg acgcgctggc cctggaaccg gtgcacgttg atgcattggc
     6361 ggtgaaactg ggcaggaaat cggaggaact tctgacgagt ctcacgctga tggagatgcg
     6421 tggccttgcg gttgcgcacg cgggttcgcg gtacagccgc gcaacgatga ccagttgagg
     6481 aggagacagc gtgcggctca ccatcggcgg aaaggttctc cttggcaacc tcggtatggt
     6541 tgcgctgatg tgtgccgcgg tgctctgggt cgttcgtgat ttgcggagga tggacgacct
     6601 ggtagaactc accgtgaatg cgcaggaaca aatcatccgg atcaacagga tctccgatga
     6661 catcaacgag atcctgtacc gcggcggaat caaagtctcg atgttccagg tgggcgcgtt
     6721 cgccgcatcg cgcgacgcgg atgagatgac gagatcgctt cgctctcggc gtgcgcaccg
     6781 tgattccctg ctggcggagg taagccggtc ccggttcggg cagcgccccg cgctgaagta
     6841 cagccttgac agcctcctca tcgcgtgccg gaagctcgat ctgctttttg aaagcgtgga
     6901 attgcacttc gaacgcggac agccgggccg ggccgagcgt gcattcagcc agttgcacga
     6961 agtggacgcc gagctttccc gggcgatgac gagtctgcag gaacacgcgc aggaggacgc
     7021 gaacgtcctc ctcgcggcgg cgtcgcggtt cagggagcat ctgatcctgc gcgcgggcgt
     7081 ggttctggcg gttctcctgg cgggaatgct cgccatggtg atgtatctga accggactat
     7141 caagcgggcg accgccggca tcagcaacgc attgagcgct gttgccagcg gcgatttcca
     7201 gcagcgcgtg gaactggtaa gcaaagacga attctccgaa atcgccgagg gtctgaatgc
     7261 cacggtggaa cgtctcggtg aacttgacgc gttgaaggcg gatttcctct ccaaggtctc
     7321 ccacgatctg cgaacgccca tcgccgcggt gaagcaggct gcggaactgc tcaacgatgg
     7381 catcccgagc ccgctcaagg aggaccagaa agaaatcctg cagatcattc gtacgaacgc
     7441 gaaacgtctc gggaacctca tcaacgacct gctcgatacg gccaggcttg aggcgggcaa
     7501 gctggaaatt caaccggaaa cgaccgacct cgtcgccatc attcgccggg ttgcaacgtc
     7561 tatcgcgcct ctttccatgg agaaacggct acggatcacc ctgaaagccg caccggacct
     7621 cccgccggcg tatgctgatc cgaaccgtat ggagcaggtg ctcatgaacc tgctcggcaa
     7681 cgcggtcaag ttcacaccgg aaaacggaga gatcgcgttg agctgcgagc aggagggcgc
     7741 gttcctgcgc tgctccgtgc gcgataccgg cgtgggtatt cccccggaag acttgccgcg
     7801 gattttcgac aaattccacc aggttcgtat gaaacgcgct acgaaggcga aaggcaccgg
     7861 cctcggcctt acgatcgtca agttcctcgt ggaagcgcac ggaggcttcg tctgggcgga
     7921 aagcaccccg gggcaggggg caacgtttac gttcacggtt cccgtcgtgg agcagcccgg
     7981 agaaggcgag gacgtccggt attcgggaac gcgcgaagag gacgcggacg tgcggccgga
     8041 ctgaagcccg cgcgtgatcg taatctggaa tgtggacagc atccggcggg agatacttcc
     8101 tgctgttgca cgattcgtgg aaacctgaca gcggtgccaa tcccgcaaat cggtgctccg
     8161 cttccgcaac gaaaggaatg gctggatttg gagcaagcgc cggcggttgc gttcttattg
     8221 cagatgccgg aattgttcct cgtaacgggc gaacgcaatc gcacgacggg tatgcatgag
     8281 gcggcgacgg ctcgttttgc agggccggtc acagccgcag aagtgggggg taacatggaa
     8341 ccgctccgcg tgtttgtcgt ggacgacgac gaggccttcg ttcaacttgt caccgccgtg
     8401 ctctccgcga agttcgggca ctgggttgcc tcggagaccg acgcagtgaa ggcggttgaa
     8461 cgcatcaaag cggaacaacc ggatgtggtc ctgctggacc tcgtcatgcc gcggcaggat
     8521 gggtatgaga ctctgaagtt ccttcttgcc gacgagaaga cgcgcgacat tccggtagtc
     8581 atgcttacca atttcgacgc ggacaaattc cgttccatgg cggaacgctg gggagccagc
     8641 gcgttcctcg gaaagaaagc gatcggcctt tccgcgtttc tcaatcgcgg agcgcaggcg
     8701 gacgatctgc cgttcgcgca gaaagatgac ctcaactacg cgcagctcga cgaagtgtta
     8761 cggaaagtag tcaccaaccg taccgcgtag cgaaccaccg gccggcacgg agaaaattgg
     8821 tcatcgccga aggagaacgt ctctgtgaag tc
//
LOCUS       DAOTNG010000117         8662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7366_length_8662_cov_5.111769, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000117 DAOTNG010000000
VERSION     DAOTNG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_8662_cov_5.111769"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5179_07075"
     CDS             complement(<1..217)
                     /locus_tag="P5179_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95014.1"
                     /translation="MRTGSRKPARRAHLPPGEAILAALSARAKPLSLQQLASAVHSTQ
                     DDPLFRQAVMDLRREGKIYEGRGQRYAS"
     gene            complement(224..997)
                     /gene="purQ"
                     /locus_tag="P5179_07080"
     CDS             complement(224..997)
                     /gene="purQ"
                     /locus_tag="P5179_07080"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HRS95015.1"
                     /translation="MRPHVLVLRAAGSNCDVETAHAFSVAGGEAERIHVNRVAEKPSL
                     LEETSILVIPGGFTYGDDVSAGKVLANELMTRLSEPLLRFVERGGLVLGICNGFQVLV
                     KTGLLPGWNEQTPSCTLAGNLSGKFEDRWVWLKPGSSRCVYTRGIEKPIYLPVAHAEG
                     RFTASSLEVLDRLETNDQVVFRYTDALGNDVGYPGNPNGADRSIAAICNVSGRVMGMM
                     PHPERHIHPTHHPQWTRNASRSRPDGLAIFENAIRAAQE"
     gene            complement(1018..1893)
                     /locus_tag="P5179_07085"
     CDS             complement(1018..1893)
                     /locus_tag="P5179_07085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRS95016.1"
                     /translation="MYPELFKFGPVTVYSYGAMLACAFIVGAWLFRRELRRWNLPEDL
                     ADRVALAAMFGGIIGAKLYFIALEAPDEFAAEPLNTLFSGAGLTFYGGLAGAVLFIWF
                     VVRRAKVSFLHVADLLGPILVLGYAFGRAGCFLSGDGDYGPPTNLPWGMSFPNGTVPT
                     VLMVHPTPLYEIGLCLVGFGYLWFVLRHKTLAPGVMVAATFLVAGVERLITEFWRFDS
                     GVYATYAYAYGGTSLAESQRVAFEIARKAHFAYAGLSLAQVLSVALVIAGVVILFVQR
                     RKPARAIPGAVGRKS"
     gene            2152..3153
                     /locus_tag="P5179_07090"
     CDS             2152..3153
                     /locus_tag="P5179_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HRS95017.1"
                     /translation="MPLLTMKQILDDAIAKNYGVGAFNINNMEQTLAIMSAADRTESP
                     VILQASRGARKYAGPVMIYGLVQAAMKLYPKVPVALHLDHGNNFETCKQSMDDGWTSV
                     MMDGSLMEDGKTPSTFEYNVDVTSRVVAEGHKRGITVEGEIGTLGGIEDGHGADRVKL
                     ADPDQAVEFAHKTGVDALALAFGTSHGAFKFKAKPVLAYDIVQAVNKKMPGLAIVSHG
                     SSSVPPELVAEINKYGGKMPGAMGVPLEDIVRSIDFGVRKINIDTDLRLAATATIRKV
                     FVETPEKFDIRDYLRPAMEAMSHVVETRMIAFRTAGKASNFTKVISLEEMAKVYAKK"
     gene            complement(3242..4204)
                     /locus_tag="P5179_07095"
     CDS             complement(3242..4204)
                     /locus_tag="P5179_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95018.1"
                     /translation="MERVRLGLIGAGGMANAVHYPSLAEMPDVEMTALCDLDEERLRK
                     TADRIGVAARYTNYRSMLDNEQVDAVYALMPPHQLFEPVMDAIERGKHVFIEKPPAVT
                     SDQCLQMATAAKNRGVHTMVAFNRRFIPVMQKAREMVTAKGDVIQVVSTFYKDYRGQP
                     PYYRGAVDILTSDMIHAVDMLRWMAGAEVVDLVSDVQSRNASYANMFNALVRFENGCV
                     GALLGNWNVGGRRHVFEMHADGISAYCNPDAAVEILADNGQPVVFSSKEAAGSEENRV
                     YYGFAAENRHFIDSLKADREPETSFGDAHKTMLLVDLIYRNAWN"
     gene            complement(4290..5315)
                     /locus_tag="P5179_07100"
     CDS             complement(4290..5315)
                     /locus_tag="P5179_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRS95019.1"
                     /translation="MATDLNAISRVIRSKNRFVVSAHMHFDGDAGASQLAMARLLRMA
                     GKEAIVVNDEETPIQFRFLPEIETILTAVPADYAPDAVVIIDTPVRENINCGDIRRRF
                     QKPVVPAEEAGTIGKSAWKDVVTIYLDHHERSDPLGEYAYVDPTASAAAVIVARLLKA
                     LKVPADSTLGTMIVCGIMSDTGRFSFANTTPEALRIVADMIDAGASVNQVATELYYKN
                     SFDSVRVTGQALSTMELSASGKVCSMLIYEEPVNAQGQAIEVEDLPNWPVTIRGVEIG
                     MFLRPGADGTVRVSIRSAGDADVDKFASQFGGGGHKKAAGCKMKIPIEEARRALVAAA
                     EKFLLEK"
     gene            complement(5476..6351)
                     /locus_tag="P5179_07105"
     CDS             complement(5476..6351)
                     /locus_tag="P5179_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRS95020.1"
                     /translation="MCAARLNEAHVFDYHHHSEFSVDCTASMADICRVAVERGVSEIA
                     FTEHMDFIPEEKNTGYFRYDAYMESIRRCREQFRDELAIVAGIEVDYCPDFENETAKW
                     VEEREFDFVIGSVHYLRGKGNISEPRALDFFRGKTEREAYGEYWDVVLRCARFGIWDT
                     LGHIDVVKRYGTQVFGPFDETRHAEALGEILSTLVTKRIALEINTSGLRQPSAECYPS
                     AALLRRYRELGGERITVGSDSHSVEHTGAGVLFGLRMARETGFAHVERFRRRQRSAVP
                     ISLEFEPGNERLLQQ"
     gene            complement(6382..7011)
                     /locus_tag="P5179_07110"
     CDS             complement(6382..7011)
                     /locus_tag="P5179_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95021.1"
                     /translation="MTGLLLEGIVASGKTSVIRAIQALPAWQRRSSKLLLSEFFTERA
                     NEHLRNRDADAYRALMHRNLRILDAARQIEMASPLLSEESGRELCYLLERFHFTNAIS
                     YADGLFETYRDIDLTLARLNCRVVVLMVEESMVSSRIAEAFRQRGQAWQAYQKRLEEK
                     VGDLGTHYVGVQRAFVSGLNWTSMEHRTIDATDKDWRRCAEEILEFWRI"
     gene            complement(7040..8050)
                     /locus_tag="P5179_07115"
     CDS             complement(7040..8050)
                     /locus_tag="P5179_07115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS95022.1"
                     /translation="MPPRAVFSALAMAVVLIFAVRAPTAPLRSPEESLRRIDETLRRP
                     ARSPFQRERNVQTARDMIARFRTEYPGQEYRGAALLYSARCAAMLEDFALAGTLADSA
                     IAMPLTDSRRMEAEFILGMASIRMGETARGADVLKELLARGVRHEIVPEARILLAQTL
                     AESGDGASATALLDSVIREGRPDWAVERARLLRPGYRRIGQPATDFAARALDGSNIRL
                     SDYAGRVVLLDFWATWCGPCRTTIPEIAQLYERYHAQGFDVIGISLDTNEEALRSFIA
                     TYRMPWQQVFEGKGWQSEIGRTYGVNGIPALFVIDRGGRIRGNELRGRPLEEMIQRLL
                     RE"
     gene            complement(8076..>8662)
                     /locus_tag="P5179_07120"
     CDS             complement(8076..>8662)
                     /locus_tag="P5179_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95023.1"
                     /translation="CAFASGNAELAKEAAVRAVVNNYHDTESWRLLGNIGNASGFSVH
                     RRRIPKRVEINEIAGRSVEIVLDSLAEPSSRAAWFAYAAVRSVWRYEGRFAGRFPRAR
                     NYFPTYDEEVEALASLISVWRATIADSAAADVQADADLEALSRIAEEGYLNEYVLFEE
                     LCADNPNTVRSFSPALLLRIREYISRYVIGGLSA"
BASE COUNT         1846 a         2755 c         2269 g         1792 t
ORIGIN      
        1 cgctggcgta tcgctggccc ctcccctcgt aaatcttacc ttcccggcgc aaatccatca
       61 ctgcctgccg aaacaatggg tcgtcctgcg tcgagtgcac tgcggaagcc agttgctgca
      121 acgacaaggg cttggcccgc gctgaaaggg ctgccaggat ggcctcaccc ggtggaaggt
      181 gtgctcgcct cgcaggcttt cgcgaaccgg ttcgcatcag cccctattcc tgcgcggcac
      241 ggatggcatt ctcgaaaatg gcgagcccgt ccgggcggct gcggctggcg ttacgcgtcc
      301 attgaggatg gtgagtagga tgaatgtggc gctccggatg cggcatcatt cccatcacgc
      361 gtccggagac gttgcagatg gcagcgatag accggtcggc accattcggg tttccgggat
      421 accccacgtc gttccccagg gcatccgtat accgaaacac aacctgatca ttcgtttcga
      481 gacggtcaag cacctccagg gatgacgcgg tgaaacgccc ctccgcatgc gcaaccggga
      541 ggtaaatcgg cttttctatg ccgcgcgtgt acacgcatcg cgagctaccc ggtttcagcc
      601 acacccagcg atcctcaaac tttccggaaa gattgcccgc gagcgtacag gacggagttt
      661 gttcgttcca tcccggcaat aggccggtct taacgagtac ctggaaaccg ttgcagatcc
      721 cgagaacgag gccaccacgc tcaacaaacc tcagaagcgg ctctgagagc cgtgtcatca
      781 gttcattcgc gagaaccttg ccggcgctga catcgtcacc gtaggtgaaa ccaccgggaa
      841 tgacaaggat tgacgtctcc tccagcaacg aaggcttttc ggcaacacgg ttgacgtgta
      901 tccgctcagc ctcgcctccg gcaacggaaa acgcatgagc ggtctccaca tcgcagtttg
      961 agcccgccgc ccgcagaacc agtacatgag gacgcatcga ttcccctgca aaaaagttta
     1021 cgacttcctg ccaaccgctc ctggaattgc tcttgcgggc ttccttcgtt gaacaaacaa
     1081 gatgaccacg cccgcgatga caagtgcaac gctaagaact tgcgcaagac tcaacccggc
     1141 atacgcgaaa tgcgccttcc tggcaatttc gaaggcaacg cgttgtgatt ccgccagcga
     1201 agtcccaccg tatgcatacg cgtatgtcgc gtataccccg gaatcgaacc gccagaactc
     1261 ggtaatgaga cgctccaccc ccgctacgag aaaggtcgct gccaccatca cccccggcgc
     1321 cagtgtcttg tggcgcaaca cgaaccagag gtacccgaag cccacaaggc aaagtccgat
     1381 ctcatacaac ggcgtcggat ggaccatcag caccgtcggc acggtcccgt tcggaaaact
     1441 cattccccag ggcaggttcg ttggcggtcc atagtccccg tcgcctgaaa gaaaacaacc
     1501 cgcacggccg aatgcgtatc cgagcaccag tatgggtccc agcaaatccg ccacatggag
     1561 gaatgacacc ttcgcccttc gcaccacgaa ccagatgaaa agaaccgcac ccgccagccc
     1621 gccgtaaaac gtaagacccg cgccggaaaa cagggtattg agaggctccg ccgcgaattc
     1681 atccggcgct tccagcgcga tgaaatacag cttcgcacca atgattccgc cgaacatcgc
     1741 cgccaacgcc accctgtcag ccaggtcctc cggtagattc catctccgca gttcgcgccg
     1801 gaacagccac gccccgacaa tgaacgcaca tgcaagcatc gcgccgtagc tgtataccgt
     1861 gacgggtcca aatttgaaca gctctggata catcgcactt cctcacatgt ccgtggacac
     1921 accaccagcg gactatcaaa tatacgatgc atcctgaagc cggtctgcgc cccctccttc
     1981 ggagttgtac accccggccg ccggtttccg gcccaggcgc cggaaagcga agcttgaccc
     2041 actctccccg tgatgtcatc tttttataat gtctttcgcg ccgacctgcg gctgcttttc
     2101 atcccggcgt tccgccggtt cccgttcgtt tcccgctgga ggatttctct tatgcccctt
     2161 ctcaccatga agcagattct ggatgacgct atcgccaaga actacggtgt cggcgccttc
     2221 aacatcaaca acatggaaca gaccctcgcc atcatgagcg ccgccgacag gactgagtcg
     2281 ccggttatcc ttcaggccag ccgcggagca cgcaaatacg ccggtccggt aatgatttac
     2341 gggctcgttc aggccgcgat gaagctttac ccgaaagtcc ccgtcgcgct ccatcttgat
     2401 cacggcaaca acttcgagac gtgcaaacaa tccatggacg atggctggac atcagtgatg
     2461 atggacggat cgctcatgga ggacgggaag acgccgtcca cattcgagta caatgtcgac
     2521 gtgacgtccc gggtggtagc cgaaggacac aagcgtggga tcaccgtaga aggggaaatc
     2581 ggcacccttg gcggtatcga agacgggcac ggagctgaca gggtaaagct ggctgatccg
     2641 gaccaggcgg tggagttcgc ccacaaaaca ggtgtagacg ccctggctct cgcgttcgga
     2701 accagccacg gcgcgttcaa attcaaggca aaaccggtgc tggcttacga catcgttcag
     2761 gcagtcaaca agaagatgcc cggtctcgcg atcgtcagcc acggttcttc gagcgttccc
     2821 cccgagctcg ttgcggaaat caacaagtac ggcggcaaaa tgcccggcgc gatgggtgtc
     2881 ccgttggagg acatcgtccg ctcaatcgat ttcggtgtcc gaaagatcaa catcgacacc
     2941 gaccttcgtc tcgcggccac tgccaccatc cgcaaagtct tcgttgaaac gccggagaag
     3001 ttcgacatcc gtgattacct caggccggcg atggaagcca tgtcgcacgt cgtcgaaaca
     3061 aggatgatcg ccttccgcac cgcgggaaag gccagcaatt tcaccaaagt gatctccctg
     3121 gaggagatgg cgaaggtgta cgcgaagaag tagtcgtcaa gatcacaaga cgtcgaaatg
     3181 caaaggggca ggccttacca aggcctgccc ctttttccgc tcgtaaagtg tccagcgttc
     3241 gtcagttcca cgcattccgg tagatcaggt cgaccaggag catcgttttg tgggcgtcac
     3301 cgaaactcgt ttctggttct cgatccgctt tgagactgtc gataaagtgc cgattctccg
     3361 cggcaaaacc gtagtacacc cggttctcct cgctgccggc cgcttccttt gatgaaaaca
     3421 cgaccggctg accgttgtcc gccagaatct ccactgcagc gtcagggttg cagtacgcgc
     3481 ttattccgtc cgcgtgcatt tcgaacacat gcctgcgccc gcccacgttc cagtttccga
     3541 gcagtgctcc gacacagccg ttttcaaaac gcaccagcgc gttgaacatg ttggcgtaac
     3601 ttgcgttcct tgactgcacg tcactcacaa gatccactac ttcagcgccc gccatccacc
     3661 gcaacatgtc aacggcatgg atcatgtcgc ttgtcaggat atccaccgca ccccggtagt
     3721 acgggggttg cccccggtaa tccttgtaaa acgtcgatac gacctggatc acatcgccct
     3781 tcgccgtaac catctccctg gccttctgca tgacagggat gaaccggcgg ttgaatgcga
     3841 ccatggtatg gactcctctg ttcttcgccg cggtcgccat ctgaaggcac tgatcggagg
     3901 ttactgctgg cggcttttcg atgaacacgt gcttgccccg ctcgatcgca tccatcaccg
     3961 gttcaaatag ctggtgcggt ggcatcagag cgtagacggc gtccacctgt tcattgtcca
     4021 gcatggaacg gtaattcgta tagcgcgccg cgacgccgat ccggtccgcg gttttgcgca
     4081 gccgctcctc gtccaggtca cacaacgctg tcatctccac gtctggcatt tccgcaaggc
     4141 tcggataatg aaccgcgttt gccattccgc ccgctccaat gaggcccagc cgaacccgtt
     4201 ccatcacgta gactcctcaa agaaaaaagg agaaggtgcg cgtctgcgcg agcccttctc
     4261 cggtttgccc atcacaagcg gattctcatt cacttttcca acaggaattt ctccgccgct
     4321 gcgacaaggg cgcggcgcgc ttcctcgatg gggatcttca ttttgcagcc tgcggccttc
     4381 ttgtgtcctc cgccgccaaa ctgagaagcg aacttgtcta cgtcggcatc gccggctgac
     4441 cgaatgctga cacgaacggt tccgtcggct cctggccgaa ggaacatccc gatttcgacc
     4501 cctctgatgg tcaccggcca gttcggcaga tcctccacct caatggcctg cccctgagca
     4561 ttcaccggtt cctcgtagat cagcatggaa cagactttgc cgctcgcgga caactccatt
     4621 gtggaaagcg cctgtccggt aacgcgcacg ctgtcgaacg agttcttgta gtacagctcc
     4681 gttgcaacct ggttgacgct ggcccctgcg tctatcatgt ccgccacgat acgaagggct
     4741 tcaggagtcg tgttcgcgaa actgaagcgc cctgtatccg acatgattcc gcacacgatc
     4801 atcgtcccca gagtggaatc tgccggcacc ttgagggcct tcaggaggcg cgcaacaatc
     4861 actgcggcgg cggacgctgt cgggtcaaca tacgcgtact ccccgagagg gtcacttctc
     4921 tcgtggtgat cgagatagat cgtgaccacg tctttccacg ccgatttccc gattgtgccg
     4981 gcctcttccg cgggaaccac cggtttctga aaacgccgcc ggatgtcccc gcagttgatg
     5041 ttctcccgaa cgggggtatc gataatcaca actgcgtcag gagcataatc cgccggaaca
     5101 gccgtcagta tcgtttcgat ttcaggcagg aatcgaaatt gaatcggcgt ctcttcatca
     5161 ttgacaacta tcgcttcctt gcctgccatg cgcaggagtc gcgccatggc aagctgcgag
     5221 gcacctgcat cgccgtcgaa atgcatatgg gcagaaacta cgaagcgatt cttcgaacgg
     5281 attacccgag agatcgcatt gagatctgtc gccatttact cctcccgcct tcgtgtaagc
     5341 ccgcgcgttg tcccggtttt gctgcacgca acacccaact cgccgctccc ttgcccgatc
     5401 ttgtccatcg cgcaagcata ccggccgcgc ggccagaaac caacgaggaa cacacacaaa
     5461 gatggctcag agcggctatt gttgcaagag ccgttcattc cccggttcga actcgagact
     5521 gattggcacc gcgcttcttt gccgtcgacg aaagcgctcc acgtgcgcaa aacccgtttc
     5581 cctcgccatg cgcagaccaa aaaggacccc ggctcctgta tgctccaccg aatgggaatc
     5641 cgaacccacg gtgatgcgct cgccgccgag ttctctgtac cttcgcaaca gcgcggcaga
     5701 cgggtaacac tcggccgagg gttgccgaag tccggacgta ttgatctcaa gcgcaatccg
     5761 cttcgtgacc agcgttgaca gaatctcgcc cagcgcctcg gcgtgtcgtg tttcgtcgaa
     5821 tggcccgaaa acctgagtgc cgtaccgttt caccacgtca atgtgcccga gtgtatccca
     5881 gattccaaag cgcgcacaac ggagtaccac gtcccagtat tcaccgtacg cctcacgttc
     5941 tgtctttcca cggaaaaagt cgagcgctcg cggttctgag atgttgccct ttccccgaag
     6001 gtagtgcacc gagccgatca cgaaatcaaa ctctctctcc tcaacccatt tcgctgtttc
     6061 gttctcaaaa tcggggcaat agtccacctc gatccccgcc acgattgcca gttcgtcacg
     6121 gaattgttca cgacatctgc gaatagactc catgtatgca tcgtatcgaa agtatcccgt
     6181 gtttttctct tccgggataa aatccatgtg ctcggtgaac gcgatctcgg atacgccgcg
     6241 ttccaccgcc acccggcaga tatctgccat ggaagcggtg cagtcgactg aaaattcgga
     6301 atgatgatgg taatcgaaca cgtgcgcctc attcaatctc gctgcacaca tcggggtgaa
     6361 gcaggttggt ctcagggttg gtcaaatgcg ccagaattcg aggatctcct cggcgcaccg
     6421 ccgccagtct ttgtcggtgg cgtcgatcgt tctgtgttcc atgcttgtcc aattcaagcc
     6481 gctgacaaag gcacgttgaa cacccacata atgcgtgccg aggtcgccca ctttttcctc
     6541 aagacgtttc tggtaggcct gccatgcctg cccccgctgt cgaaacgctt ccgctattcg
     6601 cgaggaaacc atggattcct caaccatcag aacgacgacg cggcaattca gcctcgcgag
     6661 cgtgagatcg atatcccggt acgtctcaaa aaggccgtca gcataggaaa tggcgttggt
     6721 gaaatggaac cgttccagga ggtaacacag ctcgcgtccg ctttcttccg aaagcaacgg
     6781 acttgccatt tcgatctgcc gcgccgcatc gaggatccga agattcctgt gcatcagcgc
     6841 gcggtatgca tctgcatccc ggttgcgaag atgttcgttc gcacgttcgg taaagaactc
     6901 gctcaaaagg agcttcgatg accgtctctg ccatgcgggc aacgcctgga ttgctctgat
     6961 cacgctcgtc ttgccagacg caacaatgcc ttcgagcagc agtcctgtca cgggttgact
     7021 cctcgaattc cccggctttc tactcccgga gaagacgctg gatcatttct tccagaggtc
     7081 ttccgcgcaa ttcgttaccg cgaattctcc ctccccggtc tatcacaaag agcgcgggaa
     7141 ttccgttgac accgtacgtc ctgccaatct ctgattgcca gcctttccct tcgaacacct
     7201 gttgccacgg catgcggtac gtcgcgatga acgacctcag tgcttcctcg ttcgtatcga
     7261 ggctgatccc gatcacgtca aatccctggg cgtgatagcg ttcgtacaat tgtgcgatct
     7321 ccggaatcgt ggttctgcac ggtccgcacc acgtggccca gaaatcgagc agaaccaccc
     7381 tgccagcgta atcggataaa cggatgttcg atccatccag cgcgcgagcc gcgaagtcag
     7441 tcgccggctg accgattcgc cggtagccgg gccgaagcag tctcgccctc tcaacggccc
     7501 agtccgggcg tccctcgcga atcacggaat ccagtaacgc ggtagcggag gcgccatcac
     7561 cactttctgc cagcgtctgc gcgaggagga tgcgcgcctc gggcactatt tcatgccgta
     7621 caccacgcgc cagcagttct ttgagcacat ccgccccgcg ggccgtctct cccatccgga
     7681 tcgaagccat tccaaggatg aattcggctt ccattcggcg cgagtctgtg agaggcatag
     7741 caatggcgct gtctgccaga gtgcccgcca aagcaaagtc ctccagcatc gcagcacatc
     7801 tcgcggagta cagaagcgcg gctccccggt attcctgccc cgggtattcc gtcctgaaac
     7861 gggcaatcat gtcccgcgcc gtctgaacat tgcgttcccg ctggaatggg gacctcgctg
     7921 ggcgcctgag ggtctcgtca attcgccgca gagattcctc agggcttctc aacggtgcag
     7981 tcggcgcgcg cacggcgaaa atgagaacaa cggccatggc aagcgctgaa aacactgcgc
     8041 gcggcggcat tccgttcata tccttctcct agcggtcatg ccgagagccc acctatcacg
     8101 tatcgcgaaa tgtattcccg aatcctgagc aacagcgccg gtgagaatga gcgaacggtg
     8161 ttcgggttgt cagcgcacag ttcctcgaaa agaacatact cgttcaaata accttcttct
     8221 gctattctgg acaatgcctc caggtccgca tccgcttgca catctgccgc ggccgagtct
     8281 gcaattgttg cgcgccacac ggaaatcagg ctggcgagtg cttccacttc ctcgtcgtac
     8341 gtcggaaagt aatttcgtgc tctcgggaat cgccccgcaa aacgaccttc gtatcgccag
     8401 acggagcgga ctgccgcata tgcgaaccac gctgcgcgcg aagagggctc tgcgagggaa
     8461 tccagcacga tctccacgga acggccagca atttcgttga tttcgactcg tttggggata
     8521 cgcctcctgt gaacagagaa gccgcttgcg ttgccaatgt tccccagcag cctccaggat
     8581 tcggtatcgt ggtaattgtt taccactgcc cttacggcag cctctttcgc aagttccgca
     8641 tttcctgacg caaatgcgca ct
//
LOCUS       DAOTNG010000118         8629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7394_length_8629_cov_4.651738, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000118 DAOTNG010000000
VERSION     DAOTNG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7394_length_8629_cov_4.651738"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="P5179_07125"
     CDS             complement(<1..570)
                     /locus_tag="P5179_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS95024.1"
                     /translation="MRLWPEVLDPATLFESAGLTENLAQKSVRGGVTTMTSQAIKFVL
                     GTAGTVVLARLLTPADYGLVGMVTVVINFAAMFKDAGLSLATVQRDRITREQISTLFW
                     INVLISAVLGLCVLASAPLISWFYGKPELTAVTVALSFSFIISGLTIQHQALLQRHMR
                     FGALATMQIMAQVLYLIVTIGLAWMGWRYW"
     gene            738..2207
                     /locus_tag="P5179_07130"
     CDS             738..2207
                     /locus_tag="P5179_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRS95025.1"
                     /translation="MGVLKNYSIYAVGSIAQSAASALLFPFYLVFLTPTEFGAIALIT
                     VVSEFLLLFSALGLETGLNRLYYESKERQNDLVVSVLLLRVGLAGVVLAASFAAPGRL
                     SHAILGTEAYARSIPLVAGYVLAHGVLGTILFTYRLDQRATAFVVFSLLAAVVGCGLK
                     VLFIGVYRLGVEGYWIAALVTDVILVVPMLLQGSGRYLRGRPTSALLREMLVIGFPMT
                     FAALGGIAVNMSGQYALNRFAGTELVGVYAAATRIASMFAMLLSYPIAAWWGPHVLSE
                     AEKCGPGRLRELLANYLRVLIAVGVVLLGLISLGGALIVWFPIHESYRSAVPLVGLCI
                     LPLLIQVLSYPFTTQFIYAKRTHYFASGGVLTGAVAIVLNALVTPYWGIGGVILANVA
                     AAFAGTMLYYAGGRREFPVAYDWKPVWTNGALVMGYVACAVAAMSPGASISTHVVLFA
                     ALMLSLGALAANRRVWHMFSWRISNFISERTRNGGESWF"
     gene            2198..3898
                     /locus_tag="P5179_07135"
     CDS             2198..3898
                     /locus_tag="P5179_07135"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS95026.1"
                     /translation="MVLSSHGRTNYVIVLSSQASRSEMHAATELQRFLEEMTGAHFPL
                     LDDGVPVAEKEIAVGAGRHTEALLPGVDFGAFGSEELLVKTVGKRIVIAGGRQRGTLY
                     GVYRFLEILGCRWFTAKVSRIPRVEALTVEPLDTREKPLLEYREPFFAEAFDGDWAAR
                     NLANSNSASLMARHGGKVRYGTFVHTFETLLPVAKYFDEHPEWYSEIDGKRISERPQL
                     CLTNPQVLEKVIEGVRGWIKAMPDATIFSVSQNDWTNPCQCAKCREIDEREGTPQGSI
                     LQFVNKVAEAIEDEAPHVAIDTLAYWYSRKPPKTLKPRQNVIIRLCSIECCFAHPLES
                     ECPENQKFAEDIREWSRIANRLYVWDYVTNFHNYIMPWPNSKVLGANVRFFARHNVVG
                     LFEQGSYAPGGGGEAAEMRAYVLAKLLWNPSVDENRIRDEFIDGVYGKAAGKVREYVR
                     VIHEPVADPNIHMHIFCDITNPHLTDDVIERGDALLYEAEALAESEAVRERVELAHLP
                     MHYVRIRKMTDVPEKAEERKALLERFLAIARREGIRNISEQHTIETWAQHGAIEPGKW
                     "
     gene            4023..5009
                     /locus_tag="P5179_07140"
     CDS             4023..5009
                     /locus_tag="P5179_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95027.1"
                     /translation="MSLKIGFIGAGGIAGAHVAGLRKMADVQLVAFSDVQEERAARLA
                     ADTGAKVFTDWLKMLDTEQMDAVYICVPPHVHGAPELACIERKIPFFVEKPISNDLDT
                     ATNILGKVRRAKVMTAVGYMNRYRRSLQRGKELLAGARISTLEGHWLGGTPGAPWWYR
                     KAQSGGQLVEQTTHIVDAIVYLAGRVTEVYAVGAKGTHADPPEGYDVEDATAVTLKFA
                     SGAVGSINSACSLKAGGGVGLDVFSPSVVLSYRDWNMSLKATKSRLETEDVRGEDNIF
                     EIEDRAFVEAVMKKDPALIRCNYGQAFHAHHVTMAANRSLETGMPVQLADFV"
     gene            5146..5352
                     /locus_tag="P5179_07145"
     CDS             5146..5352
                     /locus_tag="P5179_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRS95028.1"
                     /translation="MATYTINIEGMTCQHCVKAVTTALSNVAGVERVTVSLQKKQATL
                     EVDEAKFKPEAARKAVINEGYVPR"
     gene            complement(5493..7109)
                     /gene="groL"
                     /locus_tag="P5179_07150"
     CDS             complement(5493..7109)
                     /gene="groL"
                     /locus_tag="P5179_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRS95029.1"
                     /translation="MPKQLEFEVDARESLRKGLNTLADAVKVTLGPKGRNVVIDKKFG
                     APTVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFNHG
                     LKNVTAGANPMDLKRGIDAAVTAAVKAIKEQSRPISGKDDMAKVAAISANNDEEIGKL
                     IADAMEKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYQSPYFVTNPDTMETELDEPL
                     ILIYDKKISAINDLLPVLQKVAQIQKPLLIISEEMEGEALATIVVNKLRGTLKAAAVK
                     APGFGDRRKAMLEDIATLTGGRVISEEAGFKLENVVLSDLGRARRVIIDKDNTTIVEG
                     AGSREAISGRIKQIRRQIEETTSDYDREKLQERLAKLAGGVAVVNVGAATEVEMKEKK
                     ARVEDALHATRAAAEEGVVPGGGVALVRAASALDTLELTGDQKIGADIVRRALDEPLR
                     WIAMNAGQEGSIVVQKVKEGKGAFGFNAQTEVYEDLYKSGVIDPTKVVRTALENAASV
                     AGLMLTTEATITDIPEEEKAPAVPPGGGMY"
     gene            complement(7124..7414)
                     /locus_tag="P5179_07155"
     CDS             complement(7124..7414)
                     /locus_tag="P5179_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRS95030.1"
                     /translation="MNIKPLGDRVLVKPIEESEQVRGGIIIPDTAKEKPQQGEVKAVG
                     PGKRGEDGTVWPMNVAVGQIVLYGKYAGTEVKIDGEDYLIMSEGDIYGIVEK"
     gene            8019..>8629
                     /locus_tag="P5179_07160"
     CDS             8019..>8629
                     /locus_tag="P5179_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS95031.1"
                     /translation="MRQRFKWHIVLMALLVTLGTLPTTSWAALTGKIAGRVSDQSGNP
                     LPGVAVFIQGRQLGATTDSDGRYFILQVPPGTYTVEASLVGYQSVRLQNVTVNMDRTT
                     EANFSLRESTIEVDAVTVVAQRPIVQQDVTSTQLSVTTEQAQQLPVNTLLDAIGFQAG
                     IDIQNKTTISVRGSSPDQINFQVDGFQQTNPIENRSYTTMNQAL"
BASE COUNT         1732 a         2520 c         2525 g         1852 t
ORIGIN      
        1 ccagtaccgc cagcccatcc atgcgagccc gatcgtcacg atcaggtaga ggacctgcgc
       61 catgatctgc atggtcgcca gtgcgccgaa ccgcatgtgg cgctgcagga gagcctggtg
      121 ctggatcgtc agcccgctga tgatgaacga gaacgaaagc gcgaccgtga cggcggtcaa
      181 ttcgggtttg ccgtaaaacc acgagatcaa tggcgcactg gcgaggacgc agagtccgag
      241 caccgcgctg atgagtacgt tgatccaaaa caaggtgctg atttgttcgc gcgttatccg
      301 atccctctgc accgtggcga gcgatagccc ggcatccttg aacatggcag cgaagttgat
      361 caccaccgtg accatgccga ccaggccgta atccgcaggg gtaagcagtc gagcaagaac
      421 cacagtcccc gcggtgccca acacgaactt gatggcctgc gatgtcatcg tcgtgacgcc
      481 gccacgaacg gacttctgcg caaggttctc agtcaggccc gctgattcga acagagtcgc
      541 gggatcgaga acttcaggcc aaagacgcat atggaaggta aaacccggtt cgagtaatca
      601 atgacggcat caacccaagc cgcccccgtt gcacacaccg ctcccgccgg cgttctttcc
      661 tctaggcgcg gcgccggggt aaggggtcgc tctgcgcctt ggcggaacag cgaacaacgg
      721 cgaggcaatt ccacgctatg ggcgtactta aaaactactc aatttacgcg gtcgggagta
      781 tagcccagag cgcagccagc gcgttgctgt tcccgtttta cctcgtgttc ctcaccccca
      841 ccgagtttgg cgcaatcgcc ctgatcaccg tggtaagcga attcctgctc cttttttccg
      901 cgctcggact ggaaacgggg ctgaatcgcc tctattacga gagcaaagag cggcagaacg
      961 atcttgtggt gtcggtgctg ctgctgcggg tcgggttggc cggggtcgtg ctggcggcgt
     1021 cctttgctgc gcccgggcgg ctttcccacg ccattctcgg cacggaagcg tatgcgcggt
     1081 ccattccgct ggttgcgggg tatgttctgg cgcatggcgt gctcggaaca atcctgttca
     1141 cgtatcgact cgatcagagg gccactgcgt tcgtcgtctt ctcgcttctt gccgcggtcg
     1201 tgggttgcgg gttgaaagtg ctgttcatcg gcgtgtaccg tctcggtgtc gaggggtact
     1261 ggatcgcggc gcttgtcacc gacgtgattc tcgtggtgcc gatgcttttg cagggcagcg
     1321 gccggtatct tcgcggccgg cccacgtcgg cgcttctgcg ggagatgctg gtgatcggat
     1381 tccccatgac gttcgccgcg ctgggcggaa tcgcggtgaa catgtccggc cagtacgcgc
     1441 tgaaccggtt tgcgggaacc gagcttgtcg gagtctacgc ggcggccacg cggatcgcat
     1501 ccatgtttgc catgctgctt tcgtatccga ttgccgcgtg gtggggcccg catgtgcttt
     1561 ccgaagcgga gaagtgtggc cccgggcgtc ttcgggagct tctggcgaat tatctccgcg
     1621 tgctgattgc ggtgggggtg gtgctgctgg gtctcatttc gctgggtggt gcgctcatcg
     1681 tctggttccc gattcacgaa agttaccgga gtgccgttcc gctggtcggg ctttgcattc
     1741 tcccgttgct catccaggtt ctgtcctacc cctttaccac gcagttcatt tacgcgaaac
     1801 ggactcatta cttcgcctcg ggcggcgtcc tcacgggtgc cgtggcaata gtcctgaacg
     1861 cgttagtgac tccgtactgg ggaataggtg gcgtcattct tgcaaacgta gccgctgcgt
     1921 tcgcaggcac gatgctctac tacgcgggtg ggcggaggga attcccggtc gcgtacgact
     1981 ggaagcccgt ctggacaaac ggtgcgctcg tgatggggta cgtggcgtgc gcggtcgcgg
     2041 caatgtcacc gggtgcctcg atcagcaccc acgtcgtcct tttcgcggcg ctgatgcttt
     2101 cgctgggcgc tctcgcggca aaccgtcgag tatggcacat gttttcgtgg agaatttcca
     2161 atttcatctc ggaaagaact cgcaatggag gtgaatcatg gttctgagtt cacacgggcg
     2221 cacgaactac gtcatcgtcc tgtcgtccca ggcatcgcgc tcggagatgc acgccgcaac
     2281 cgagctgcag cgcttcctcg aggaaatgac gggagcccat ttccccttgc tcgatgacgg
     2341 ggtgcctgtc gcggagaagg aaatcgcggt cggcgcaggc cgtcacaccg aggccctgct
     2401 tcccggcgtc gacttcggcg cattcgggtc tgaggagctg ctggtcaaaa cggtgggcaa
     2461 gcgcatcgtc atagcaggag ggcgtcaacg ggggaccctg tacggggtgt accggttcct
     2521 tgagatactg gggtgccgtt ggttcaccgc aaaagtctcc cggatcccgc gcgtggaggc
     2581 cctgacggtg gagcctctgg acacaaggga aaagccactt ttggagtacc gggagccgtt
     2641 tttcgcagag gcgttcgacg gcgattgggc ggcgcgaaac ctcgcgaatt ccaacagcgc
     2701 cagcctcatg gcccggcacg gcgggaaggt cagatacggc acattcgtgc acacgttcga
     2761 aacgctcttg cccgtcgcga agtactttga cgagcatccc gagtggtaca gcgaaattga
     2821 cggcaaacgt atctctgagc ggccgcagct ctgtctgacg aatccgcagg tgctggaaaa
     2881 agtgattgag ggggtgcgcg ggtggatcaa agcgatgccc gacgccacca ttttcagcgt
     2941 aagccagaat gactggacga atccctgcca gtgcgcgaaa tgccgcgaaa tcgacgaacg
     3001 ggaaggcaca ccgcagggaa gtattctcca gtttgtgaac aaggttgccg aggcgattga
     3061 agacgaagcg ccgcatgtgg caattgatac cctcgcgtac tggtattccc gcaagccacc
     3121 gaagacgttg aagccacgcc aaaacgtcat aattcgtctc tgctcaatcg agtgctgctt
     3181 tgcccaccca ctggaatccg agtgcccgga gaaccagaaa ttcgcggagg atatccgcga
     3241 atggagcaga atcgcgaacc ggttgtatgt gtgggattac gtcacgaact tccacaacta
     3301 cataatgccg tggccgaatt ccaaggttct gggcgcgaac gtccgtttct tcgcccgcca
     3361 caacgtggtc ggcctgttcg aacaggggag ctacgcaccg ggcggcggcg gtgaggcggc
     3421 ggagatgcgc gcctacgtgc tggccaaact gctctggaac cccagcgtgg atgagaaccg
     3481 gatccgtgac gagttcatcg atggcgtgta cggaaaagcc gctggcaagg ttcgggagta
     3541 tgtcagggta atccatgaac cagttgccga cccgaacatt cacatgcata tcttctgcga
     3601 catcacaaat ccgcacctta ccgacgacgt gatcgagcgc ggcgacgcac tgctgtacga
     3661 ggctgaagcg cttgcggaat ccgaggccgt tcgcgaacgg gtggagctgg cgcatctgcc
     3721 catgcattat gttcgaatcc gaaaaatgac cgatgttccg gagaaagcgg aggaacgcaa
     3781 agccctgttg gagagattcc tcgccatcgc cagacgggag ggcatccgga acatctcgga
     3841 acagcacacg atagaaacct gggcgcagca cggggcgatc gaaccgggca aatggtaatc
     3901 tggcaacgtt ccaccgcaag caccctttct cccggcggag aaagggtgtt ttcttttgag
     3961 tggaatcgga gaaggtagct ctcgttcaca aagacccccg cagcgtaagg aaaggactcg
     4021 aaatgtcgct gaagatcggt ttcatcggcg ccggaggcat tgccggagca cacgtggctg
     4081 ggctcaggaa aatggcggac gtccagcttg tggcgttttc tgacgtccag gaggaacgtg
     4141 ccgctcgcct tgcggcggac accggagcaa aggtgttcac cgactggctg aagatgctcg
     4201 acaccgagca gatggatgcg gtctatattt gtgtgcctcc gcacgtacac ggcgcgccgg
     4261 aactcgcctg catcgaacgc aaaataccct tcttcgtgga aaaaccaatc agcaacgatc
     4321 tcgacacggc aaccaacatc ctcggcaaag tgcgccgcgc caaagtcatg acggcggtcg
     4381 ggtacatgaa ccgttaccgg cgcagcctcc agagaggaaa agaactgctc gcgggagcca
     4441 ggatttcgac tctggaaggc cattggctgg ggggaacacc gggagcaccg tggtggtacc
     4501 gcaaggcgca atccggcggt caactcgtgg agcagacaac gcacatcgtt gacgccatcg
     4561 tctatcttgc gggacgcgtg acagaagtgt acgccgtcgg ggcaaaaggg actcacgcag
     4621 acccaccgga agggtatgat gttgaggacg cgactgccgt gacgctcaag ttcgcaagcg
     4681 gcgccgttgg ttccatcaac tcggcatgtt cgctcaaggc gggaggaggg gtcggactgg
     4741 acgtgttcag cccgtcagtg gttctctcat accgagattg gaacatgagt ctcaaagcga
     4801 ccaaatcccg tctggagaca gaggacgtgc gcggcgaaga caacatcttc gaaatcgaag
     4861 atcgggcctt tgtggaggcc gtgatgaaaa aggaccctgc actgattcgc tgcaactacg
     4921 gtcaggcgtt ccacgcccac catgtgacga tggcggcaaa ccgctctctg gagacaggaa
     4981 tgcctgtgca attagccgat ttcgtctagc agatcaaacc ggaatctcga tgaagcgggt
     5041 gcggtgccag ccgccccttt ccttttgtca tgtgtcccgg aacgaagagc cgggccgaca
     5101 ttgttctatt tgatggacct gcgggatttc ggaaggagat tgaaattggc aacgtacacg
     5161 ataaacatcg agggcatgac gtgtcagcat tgcgtcaaag cggtcactac ggctctgtcc
     5221 aatgtcgccg gcgtcgaacg cgtcacggtg tccctgcaaa agaagcaggc aacgcttgaa
     5281 gtggacgagg cgaaattcaa acctgaagcc gcgcggaagg cggtgattaa tgaaggctac
     5341 gtgcccagat aggtagaagg cctgggaact gacccgccgc cagtgaacac ggagttcatc
     5401 cgtttccccg gggtgcgagc ggcaaaatag aagcagggcg cgacctttcg gccgcgccct
     5461 gcttccgttc gtggaggttc gcagttatga tctcagtaca tcccgccgcc cgggggcacc
     5521 gcgggagcct tctcttcttc gggaatgtcg gtgattgttg cttccgtggt gagcatgagg
     5581 cccgcaacgc tcgccgcatt ctccagggcg gtgcgcacga ccttggtcgg gtcgatcaca
     5641 ccggacttgt acaggtcctc gtacacctcg gtctgggcat tgaagccaaa cgctcctttg
     5701 ccttccttca ccttctggac tacgattgag ccttcctggc cggcgttcat ggcaatccac
     5761 cggagaggtt cgtccagagc gcgccgaaca atatccgcac cgattttctg atcgccggtg
     5821 agctcgagag tatccagcgc agaagccgct cggacgagcg ccacgccacc acccggcaca
     5881 acaccctctt cagcggctgc tctcgtcgcg tgcagtgcat cctcaacgcg cgccttcttc
     5941 tccttcatct ccacttccgt ggcagcacca acattgacaa ccgcaacgcc gccggcgagt
     6001 ttcgcgagcc tttcctgcag cttttcgcgg tcgtaatcac tggtggtttc ctcgatctgt
     6061 cgccgaatct gcttgatccg gccgctgatc gcttcgcgcg atcccgcgcc ttcgacaatc
     6121 gtcgtgttgt ccttgtcgat gatcacacgg cgcgcacgtc cgaggtcgct cagcacgaca
     6181 ttttcgagtt tgaacccggc ttcctcgctg atcacacgtc cgcccgtaag cgtggcgatg
     6241 tcttccagca tggctttgcg gcggtcacca aatccgggcg ccttcaccgc cgccgctttc
     6301 agcgtgccgc gcagcttgtt cactacgatg gtggccagcg cctcgccttc catttcctcg
     6361 gaaatgatca gcaacggttt ctgaatctga gccaccttct ggaggaccgg aagcaggtcg
     6421 ttgatcgcgg agatcttttt gtcataaatc agaatgagcg gttcatccag ttccgtctcc
     6481 atcgtgtccg gattggtgac aaagtacggt gactggtaac cgcggtcgaa ctgcatcccc
     6541 tcgaccacgt cgagcgtagt ctcgatgctt tttgcttctt ctacggtgat aacaccgtct
     6601 ttcccaactt tttccatcgc gtcggcgatc agcttgccga tttcctcgtc gttgttcgcg
     6661 ctgattgcgg caaccttcgc catgtcgtcc ttgccggaaa tcgggcggct ctgttctttg
     6721 atcgccttca ctgctgccgt caccgcagcg tctattccgc gcttcagatc catcgggttg
     6781 gcgcccgcgg taacattttt caggccatgg ttgaaaatcg cctgcgcgag aacggttgcc
     6841 gtagtcgttc cgtcaccggc tacgtcagag gttttggaag caacttcctt caccatctgc
     6901 gcacccatgt tttcgaactt gtcttcgagt tcgatttctt tcgcaacggt aacgccgtct
     6961 ttggtgacgg tcggagcgcc gaatttcttg tcaattacca cgttccgccc tttgggaccg
     7021 agcgtgactt tgaccgcatc ggcgagtgtg ttcaagcctt tgcgaaggct ctcacgggcg
     7081 tctacttcaa attcgagttg ttttggcatg ggaatctcct cgattacttc tcaacgatgc
     7141 cgtagatgtc gccttcggac atgatcaggt aatcttcgcc atcaatcttc acttccgtgc
     7201 ccgcgtattt cccgtagagc acgatctgcc ctaccgcgac gttcatgggc cacacagtgc
     7261 cgtcttcgcc gcgttttccc ggcccgactg ccttgacttc gccctgttgc ggtttttcct
     7321 tcgcagtgtc agggatgatg atccccccgc gcacctgctc gctctcttcg attggcttga
     7381 ccagaacgcg gtcacccagt ggtttgatgt tcatgtgcga tctcctctca tgacgaaaat
     7441 cacaggagtt cggttagttc gtccgacata ttagcactca ggtaacgcga gtgctaacaa
     7501 cgcttgctaa agtataggac tggcagccgg gtttgtcaag tgaatcatgt cgcttcgtcc
     7561 acttttttct ctccctgagg agttccaaaa gctgaggggt tcggattgta cggccccgct
     7621 cttacacttt cccaagaatt aaacattaaa aaccccattc gcccttgaca cgcgcaggaa
     7681 tgcacgccat cattattccg attgtgccct tcctaccttg ttcggggccg ttcgaggaaa
     7741 ccagttcaga accgctcgga gtgatcaaaa acatgcgcgt ccgcacgatg acattgttcc
     7801 tgcttcggcg ctccccgcgc cggtgcctga acgaatcaac ggcacgcgct gcgcaacccg
     7861 accagaccac actcctgcca tctttcccga actttcgtcc attttcccaa acctgcgctc
     7921 accacgtctt ttcaagaaag gagtaatgcg aaaggcacga gcgtttttgt gtttcaccaa
     7981 atggttgtgg tcgtcaaagt gagaggagag gagttctcat gagacagcgg ttcaaatggc
     8041 acatcgtgct gatggcgctt ctcgtgacac tggggacgct gcccacgact tcctgggcgg
     8101 ctcttaccgg gaaaatcgca ggtcgagtat ccgaccagtc cggtaacccg ctccccggtg
     8161 tagcggtgtt catccaggga cgccagctcg gcgcaacaac ggattcagac ggacggtatt
     8221 tcattctgca ggtgccgccc ggaacgtaca cggtcgaagc gagccttgta gggtaccaga
     8281 gcgttcgcct gcagaacgtc acggtcaaca tggaccggac gacggaggcg aatttctcgc
     8341 tgagagagtc cacgatcgaa gtggacgcag taaccgtcgt ggcacaacgt ccaattgttc
     8401 aacaggacgt aacgtccacc cagctctccg tcacgacgga acaggcgcaa cagcttccgg
     8461 tcaacacgct tctggatgca attggttttc aagccggtat tgacattcag aacaaaacga
     8521 cgatcagcgt gcgtggaagc tcacccgacc agattaattt ccaggtggac ggtttccagc
     8581 agaccaaccc gattgaaaac cgttcgtaca ccacgatgaa ccaggcgct
//
LOCUS       DAOTNG010000119         8577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7447_length_8577_cov_5.974184, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000119 DAOTNG010000000
VERSION     DAOTNG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_8577_cov_5.974184"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1509
                     /locus_tag="P5179_07165"
     CDS             4..1509
                     /locus_tag="P5179_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRS95032.1"
                     /translation="MFLLLAVRKTPADIAGVIGWLLTVGIAWAFFETPLSVILLASLA
                     GVIASFPITIMVGTSILQITVMQEAGAIARVVTLIKTLSPRDKVAQIMIINVGFGTLL
                     AALGATPVSILPPIMIGLGYSSFAAIALPAIGYDALCTYALLGIPAVVFSGFVGAPVT
                     EAGGYFARYMPVISTCIALGMLWIAGGWRLVRRGVFPAIVAGMTAGIVAIGMNAMGLV
                     TITGIASGVAVVVVMALYLLLLRKPVIDRAVMTDSDRAVERKMPLSAALSPWIILTAF
                     ALVVNAPFLPFFGLTFKTWAMPVTIVPGNPEKVRLFWQAYFWVLVSTVLALPFLRLSR
                     KQLGATMVKWSHRALRPMLASAVFFAIALVMNHSGKTADWQLLNPAHNMIAVLASSAA
                     NSFGAFYPAIAPFLGLFAGFVSGSEASAIAMLTKLHLETAGKIGAVGLVIAAASGIGG
                     GLASVISPAKLQNAAAAIDRIGEEGSVIRTTFVISLIITAVCAIMTLFWAF"
     gene            1520..2278
                     /gene="pgl"
                     /locus_tag="P5179_07170"
     CDS             1520..2278
                     /gene="pgl"
                     /locus_tag="P5179_07170"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRS95033.1"
                     /translation="MNRHCGGKIRLCEARTELAIAAASFVAEVIAGAVNARGFCSLVL
                     AGGNTPREVYSLLAAPPFRETIPWQDVEVFFGDERCVPPDDPQSNYRMAFQTLLSKVP
                     VRAVHRMEGEAVPQTAALRYGEILEGVFGKGVPRFDLILLGMGADGHTASLFPGTDVL
                     KENGNPVAAVWVPKLGSWRITLTLPVINNARTVAVLVSGNDKADALNRVWNASEPDPQ
                     LPVTLVCPVAGELHWFVDGDAARYLRSERRKDKS"
     gene            2275..3690
                     /gene="gndA"
                     /locus_tag="P5179_07175"
     CDS             2275..3690
                     /gene="gndA"
                     /locus_tag="P5179_07175"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRS95034.1"
                     /translation="MKNCQFGLIGLGVMGQNFVLNVERNGCRVAVYNRTAATTKAFVE
                     GAARGKKIQPAYTLEEFVGALERPRRIMLLVKAGEPVDDMIRRLSPLLAKGDLIIDGG
                     NSFYRHTEKRTHEVEASGLLYLGMGVSGGEEGALWGPSLMPGGSEAAYKLVEPLLKRV
                     AAKAGEDGKPCVAYIGPGGAGHFVKMVHNGIEYGDMQLIAEAYHLLKTVLGLTADDFS
                     RVFAEWNEGELSSYLIELTAKVFAKKDDETGKPLVDVILDSAGQKGTGKWTTQCSLEL
                     GAPVPTITAAVDARLLSAQKQERMHAAGILPGPEPGTKRVPKKALINAVRDALYVSKI
                     CSYAQGMSLLGAASKAHSYQINLGVVAEIWRAGCIIRARLLNDIKTAYDRDAALPNLL
                     LDEFFRDAVATRQAAWRQVLQTGIAHGVPMPAMAASLAYYDAYRSERLPANLIQAQRD
                     FFGAHTFERIDRPGVFHVDWE"
     gene            3743..4843
                     /locus_tag="P5179_07180"
     CDS             3743..4843
                     /locus_tag="P5179_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95035.1"
                     /translation="MKPVGIGVVGCGNISEVYLKVARNFGILKLVAVADAIPERAQAK
                     AEAHGLFACSVKDLLANPEIEIVLNLTVPAAHAEIGMAALEAGKCVYNEKPLATRRED
                     AQAMLALADRKGLLVGGAPDTFMGAGLQTCRKIIDDGWIGEPVAATAFFMGHGAERWH
                     PDPEFFYKPGAGPMFDMGPYYLTALVSLIGPVKSVCSAARASFQERVIGSQPKAGQII
                     KVETPTHIAGSMEFQSGAIATVVTSFDVWAHSLPNIEIYGTTGSLRVPDPNTFGGQVF
                     VRRFDAKEWVEVPYSHGYSEQNRSIGLADMACALRTGRSFRPNGRLTYHVLDTMHAFL
                     DSARERRHVDLASTCDRPAALPMNLSPGSLGD"
     gene            4914..5753
                     /locus_tag="P5179_07185"
     CDS             4914..5753
                     /locus_tag="P5179_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS95036.1"
                     /translation="MPPSRRFVLVVFADVHGNLPALEAAAEDAKFFCPDAVVHGGDIV
                     NGPGSREVVAFFLAKGWNGVMGNHEDYVLRCTSPRAPALWRSERFAPVRWARSHLSPR
                     HLDWLAALPNTISPHPETTVVHGSPGDFRAALRSEMDDERAVALYSGLSSRVVICGHT
                     HLPHVREIGDRLYVNAGSCGITLDGDARASYAVLTQLNENWCGEIRRVEYDTRQVLDL
                     ARRTSWLEEGGGVSATMVHEMMTGISWTTPFVRWWTTQCRERTSVDSYREFARMRGMI
                     PLL"
     gene            5852..6526
                     /locus_tag="P5179_07190"
     CDS             5852..6526
                     /locus_tag="P5179_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95037.1"
                     /translation="MVTAQARALFCPIGWPGLLFSALCGVILLGGIQSASAGESSGET
                     APWRFRVGLATVGVRHLDVVGVGITGEYRHPLAWRGVQSGGEALLMHSGERRIPTREA
                     CNDFFLNDTAYVSHVYEQVGSVGIGGSLYVPVLKGPDHVGFRVPGVMVGLSGGLMFET
                     DRVGLQSSDIQAYYGYSTPSDTDVSLRPYVRPQIVFMQGGFGLSGSAIIFPRFARWTL
                     GVAYAW"
     gene            6674..7918
                     /locus_tag="P5179_07195"
     CDS             6674..7918
                     /locus_tag="P5179_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95038.1"
                     /translation="MSDLVRTGLRSFCLFGASIALLSFLGCPPPSATTSPAEAPAVSQ
                     RVLDSLRADTLMRCRYHRSFAYQYARSGLQQDAITQFQKAIVYCKDDPEVERFYAQYL
                     DQWGKRDSAFVHYRKAGDLDTCHVLTHFWLYSFYHDAGDYQAAIRELLIAARNQEDPA
                     IKYRWLRNAADMMASENMKEESCQIYAELQQRDPTDGELAQRMLACVGDDPEKRLSTL
                     RAACMADTTNRDMCRLYAQEEERAGNDATALGIYLRFAREDSNNVGSWETVLRTAKRV
                     NSNEVILLSLRQLARLEPNSAERWGAVADQLGAENRFNEAWALVQQKLREFPENPHLL
                     YLAGFYYNRAGNKRSALEVLDRAVLTDDPTWRGLSLNLHDSIEPPLSEDEINQAKFFG
                     RKVPRIHRCRIPGREKQNEVMQ"
     gene            8063..>8577
                     /locus_tag="P5179_07200"
     CDS             8063..>8577
                     /locus_tag="P5179_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95039.1"
                     /translation="MGGRKVLTIAAVSALLFGMASAQTGAPAQGGAAQSADTAKVQVS
                     FVVTQDGKETAILEKGKLVEVVATVKLNLPDAKNANVQVIADIADQLNIENEADVKVK
                     GLYIDNGSGFSLQPTSSVRPRLGFLAPEGQTGISATQRVKWVLDFINVAPRAPFSVNA
                     AVVIPPSNEIIG"
BASE COUNT         1656 a         2413 c         2692 g         1816 t
ORIGIN      
        1 atcgtgttcc ttctcctggc ggtccggaag acgcctgcgg atattgccgg cgttattgga
       61 tggcttttga ccgtcggcat cgcgtgggcg ttttttgaaa ccccgctttc cgttattctg
      121 ctggccagtc tggcaggcgt cattgcgtcg tttcccatca ccatcatggt gggtacctcg
      181 atcctccaga tcaccgtcat gcaggaggcc ggagcgatcg cgcgcgtcgt tacgctgata
      241 aagacgctct cgccacgtga caaagtggcc cagatcatga tcatcaacgt gggtttcggc
      301 actcttctgg cagcgctcgg ggcgactccg gtttcgatcc tgcctccgat catgatcggt
      361 ctcggatact cgtcgttcgc ggccatcgcc ctccccgcaa tcgggtacga cgccctttgc
      421 acctatgcgc ttcttggcat tccggccgtc gtgttctcgg gtttcgtggg tgccccggtc
      481 accgaagcgg gtggctattt cgcccgttat atgccggtca tcagcacgtg tatcgcgctg
      541 ggtatgctgt ggatagccgg gggatggcgg ctggttcgca ggggagtgtt ccccgctatc
      601 gtggcaggga tgacggcggg aatcgtggcg atcggaatga acgcgatggg attggtcacg
      661 atcacgggga ttgcctccgg agtggcggtc gtggtagtca tggcgctcta cctgctgctc
      721 ctcagaaaac cggtaattga ccgggccgtg atgacggatt cggatcgggc ggttgaacgc
      781 aaaatgccgc tttcggcagc tctctctccc tggataatcc tgaccgcgtt cgctcttgtc
      841 gtgaacgcgc ccttccttcc gtttttcggc cttacgttca agacttgggc gatgccagtt
      901 acgatcgttc cgggcaaccc ggaaaaggtg aggcttttct ggcaggcgta tttctgggtt
      961 ctcgtaagca ccgtgctggc gctgccattc ctcagattga gtcggaaaca gcttggcgct
     1021 accatggtga aatggtccca cagagcgctg cgaccgatgc tcgcttccgc ggtcttcttt
     1081 gccattgctc tggtgatgaa ccactccggt aagacggcag actggcagct tttgaacccg
     1141 gcccacaaca tgattgcagt gctggcatca tccgcggcga actcgttcgg ggcgttctat
     1201 ccggcaattg cgcctttcct cgggcttttt gccgggtttg tcagcgggtc ggaggcatcg
     1261 gcaattgcca tgttgaccaa actgcacctc gaaacggcag ggaaaatcgg tgcagtcggc
     1321 cttgttatcg ctgcggcaag tggtatcggc ggcggtctgg cgagtgtgat ttcgccggcg
     1381 aaacttcaga atgcagcggc agcaatagac cgtatcggcg aggaggggag cgtcatccgc
     1441 accacgttcg tgatttcgct gatcatcacc gcagtgtgcg caatcatgac gctcttctgg
     1501 gcgttctgag gggactccgt tgaaccggca ctgcgggggc aagatcaggc tgtgtgaggc
     1561 gaggaccgaa ctcgccatcg cggcggcgtc gttcgttgcg gaggtgattg ccggtgccgt
     1621 caacgctcgg ggattttgtt cgcttgttct cgcaggcgga aacacgccac gcgaagtgta
     1681 ttccctgctg gccgcgccgc cgttccggga gacaatcccc tggcaggatg tggaggtatt
     1741 tttcggggac gaacggtgtg tccctccgga cgatccgcag agcaactacc ggatggcgtt
     1801 ccagacactg ttatccaagg ttccggtgag ggcggtgcac cgcatggaag gtgaagcagt
     1861 tccgcagacc gccgctttgc gctacggaga gattctggag ggggtgttcg ggaaaggcgt
     1921 tccgcgtttc gatcttatac tactgggcat gggtgccgac ggtcacacgg cgtccctgtt
     1981 tccgggaacg gacgtgttga aagagaatgg gaacccggtt gcggcggtgt gggtacccaa
     2041 gctcggttcc tggagaatca cgctcactct gccggtaatc aataatgccc gcactgtggc
     2101 agttctggtt tccggaaacg acaaagcaga cgcgctgaat cgcgtctgga atgcatcgga
     2161 accggatccg cagcttcctg tgacgttggt ttgtcccgtg gcaggcgagc ttcactggtt
     2221 tgtcgacggg gacgcggcgc gctacctgcg cagcgagagg agaaaggaca agtcatgaaa
     2281 aactgtcagt tcggcctcat cggcctcggt gtcatgggac agaacttcgt gctgaacgtg
     2341 gagcgcaacg gctgcagagt cgccgtctac aaccgaactg ccgcgacgac caaagcgttc
     2401 gtggaagggg cggcgcgcgg gaaaaagatc caaccggcct atacgctgga ggaatttgtc
     2461 ggcgcgctcg aacgcccgcg gcggatcatg cttctggtga aagcgggcga acccgtggat
     2521 gacatgatcc ggcgcctgag cccgttgctc gcaaaaggcg acctcatcat tgatggcggg
     2581 aactcattct accgtcacac cgagaaacga acccacgaag tggaggccag tgggcttctg
     2641 tacctcggca tgggcgtttc cggtggtgag gaaggtgctc tttggggccc gagtctgatg
     2701 ccgggcggtt cagaggctgc atacaagctc gtcgagccgc tgttgaagcg tgtggcggca
     2761 aaagccggag aggatgggaa accctgtgtg gcctatatag ggcccggagg ggccggccat
     2821 tttgtcaaaa tggttcacaa cggcattgaa tatggtgata tgcagctcat tgcggaggcg
     2881 taccacctcc tcaagacagt cctcggactc actgcggatg atttctcgcg cgtgtttgcg
     2941 gaatggaacg aaggagagct ctcctcgtac ctcatcgaac tgacggcgaa agtattcgcg
     3001 aaaaaggacg acgaaacggg caagccgctc gtggatgtca ttctcgacag tgcgggtcag
     3061 aaaggcacgg gcaaatggac tacgcagtgc tctctcgaac tcggagcgcc cgtgcccacg
     3121 attactgcgg ctgttgatgc tcgcctactg agcgcgcaga agcaggaacg aatgcacgcg
     3181 gccgggatcc tgccgggacc cgagccggga acgaagcgcg tgccgaaaaa ggcgctcatc
     3241 aacgcggtgc gcgacgcgct ctacgtctcg aagatctgct cgtatgctca gggcatgagc
     3301 cttctgggag cggcgtcaaa ggcacacagt taccagatta atctcggggt ggtagccgaa
     3361 atctggaggg ccgggtgcat tatccgtgcg cgcctgctga acgacatcaa aaccgcgtac
     3421 gatcgagacg ccgcattgcc gaatcttctt ctggacgagt tcttccgcga tgccgtcgca
     3481 acaaggcagg cagcctggcg gcaggtgctg cagaccggga tcgcccacgg cgtccccatg
     3541 ccggcgatgg ccgcgtctct cgcctattat gacgcgtacc gcagcgaacg cctgccggcg
     3601 aatctcattc aggcacaacg ggatttcttc ggggcacaca cgtttgaacg aattgaccgc
     3661 ccgggtgttt ttcacgtgga ctgggaataa tccggcaccc cggcgggaca acagcgcgaa
     3721 ccgcgtaaaa ggagactcac acgtgaaacc agttggaatt ggcgtggtcg ggtgcggcaa
     3781 catttccgag gtatatctca aagttgccag gaacttcgga atcctgaaac tggtcgccgt
     3841 tgcggatgcg attcccgagc gcgcacaggc gaaggcggaa gcccacgggt tgttcgcctg
     3901 ttcggtgaag gacctgcttg ccaacccaga aatcgagatt gtgctcaatc tgaccgttcc
     3961 ggcggcgcac gcggaaatcg gaatggccgc gcttgaagcg ggcaaatgcg tgtacaatga
     4021 aaagcctctg gctacccggc gcgaagacgc gcaggcgatg cttgcgcttg cggacaggaa
     4081 ggggctcctc gttggcgggg cgccggacac gttcatgggc gcgggattgc agacctgccg
     4141 gaaaatcatc gacgatggct ggataggcga acccgtggcg gcaactgcat ttttcatggg
     4201 tcacggcgcc gaaagatggc atccggaccc ggagttcttc tacaaaccgg gggccggtcc
     4261 catgttcgat atggggccgt attacctcac cgcgctggtt tccctgattg gtcccgtgaa
     4321 atcggtgtgc agcgcagcga gggcgtcgtt ccaggaacga gtgattggaa gccagcccaa
     4381 ggccggacag atcatcaagg tggagacgcc tacgcacatc gcgggaagca tggaattcca
     4441 gagcggcgcc atcgcgaccg tagtaacgag tttcgatgtg tgggctcatt cgcttccgaa
     4501 tatcgagatc tatggcacga ctggatcgct ccgcgttcct gaccccaaca cgtttggcgg
     4561 ccaggtattc gtcaggcgtt tcgacgccaa ggaatgggtc gaagtgccct actcgcatgg
     4621 gtacagcgaa cagaacagga gcatcgggct ggccgacatg gcgtgtgcgc tgcgcacggg
     4681 gcgctccttc cgcccgaacg ggaggctcac ctatcacgtg ctcgatacca tgcacgcgtt
     4741 tctggattct gcgcgcgaac gccgtcatgt tgatcttgcc agcacgtgcg accgtcccgc
     4801 ggcacttccg atgaaccttt cgcccggcag cctcggcgac tgacggtgtt caccccttcc
     4861 atcctgccac aaatccggtt ggggcaggat gggaggggtt tgcctggact gtcatgcctc
     4921 cctctcgtcg tttcgttctt gtcgtgttcg ccgacgttca cgggaacctc ccggccctcg
     4981 aagccgcggc agaggacgcg aaattctttt gcccggatgc ggtggtgcac ggtggggaca
     5041 tcgtgaacgg gccgggttcg cgcgaagtgg ttgccttctt cctcgcgaag ggatggaacg
     5101 gcgtaatggg caaccacgag gactatgtgc tccggtgtac ctccccccgg gcgcccgcgt
     5161 tatggagatc cgagcggttt gcaccggtca ggtgggctcg ctcacacctt tcgccccgcc
     5221 accttgattg gcttgccgca cttccgaaca cgatatcacc ccacccggag acaaccgttg
     5281 ttcatggctc tccgggcgat ttccgcgcgg cactccgcag cgagatggat gatgagcggg
     5341 cggtcgcact ctattcaggc ctgtcctcac gcgtggtcat ctgcgggcac acgcacctgc
     5401 cacacgttcg cgaaattgga gacaggctgt acgtcaacgc cggctcgtgc ggaatcaccc
     5461 tcgacggaga cgcaagagca tcgtacgcgg tactcacgca actgaacgaa aactggtgcg
     5521 gtgaaattcg gcgggtggag tacgacacac ggcaagtcct tgacctggcg cggcgcacct
     5581 cgtggcttga agaaggggga ggtgtctctg ccaccatggt tcacgagatg atgacgggga
     5641 taagctggac cacgcctttt gtgcggtggt ggaccacgca atgccgggaa cggacgagcg
     5701 tcgattccta ccgcgagttc gcgcgaatgc gcgggatgat tcccttgctc taggtccact
     5761 catcttcacg cgcgcttgcc gttgagttat ctttattata tccacctcga tctttttgat
     5821 gctttcgtat gatttgcctt accggttgag aatggtgacc gcacaagctc gcgctctgtt
     5881 ctgcccgatt ggttggcccg gcttgctctt ttctgctttg tgcggggtaa ttctgctcgg
     5941 cggaattcag agcgcctccg cgggggagag ctcaggcgaa acagctccct ggcgcttccg
     6001 tgtcgggctt gccaccgttg gtgttcgcca ccttgacgtg gtgggagtcg gcatcacggg
     6061 agagtaccgc cacccactgg cgtggcgcgg ggtacagagt ggcggagagg cgttgctgat
     6121 gcattccggc gaacgccgaa ttcccacgcg ggaagcctgc aacgattttt ttctgaatga
     6181 taccgcgtac gtgtcgcatg tgtatgagca ggtgggcagc gttggcatcg ggggatcact
     6241 ctacgttccc gttctcaaag gccccgacca tgtcggtttt cgcgttccgg gtgtgatggt
     6301 tggcctttcc ggcggcctga tgttcgaaac agatcgcgta gggttgcaat caagcgatat
     6361 acaggcatac tatgggtaca gcacacccag tgataccgat gtttctctcc gcccgtatgt
     6421 tcgaccgcag atcgtgttca tgcagggcgg tttcggcctt tccgggagtg caatcatttt
     6481 tcctcgattc gccaggtgga cactcggcgt agcgtacgcc tggtagattg tctcccgggg
     6541 taaccttgga accgcgcgcc cgtttttcta attttattct cctatccctc ctggctgcgg
     6601 gggagggcgg atgttccggc ggcagccgcc gtatcccgga gacgccgcgt aacaaatcgt
     6661 gagggctggt tgcatgtctg accttgtcag aaccgggttg cgttcttttt gtcttttcgg
     6721 cgcatccatc gcgcttttgt cgtttctagg gtgtcctccc ccctcggcga ccacctctcc
     6781 cgccgaagcc cccgcggtaa gccagagggt cctcgattcg ctccgtgctg acaccctgat
     6841 gagatgtcgg taccatagaa gtttcgcgta ccagtatgcg cgtagcgggc tgcaacagga
     6901 tgcgatcacg cagtttcaga aggcgattgt ttactgcaag gacgatccgg aagtggagcg
     6961 tttttacgcc cagtacctcg atcagtgggg gaaacgcgac agtgcgttcg tccattaccg
     7021 gaaggcgggg gacctggata cctgccacgt tctcacccat ttctggctct acagcttcta
     7081 ccacgacgcc ggtgattatc aggcggcgat tcgggagctt ctgattgcgg cccgcaatca
     7141 ggaagacccc gcgataaagt accgctggct ccgtaatgcc gcggacatga tggcttccga
     7201 gaacatgaag gaagaatcct gccagattta cgcggagctc cagcagcgcg acccgaccga
     7261 cggcgaactt gcgcagagga tgcttgcgtg cgtaggggat gaccctgaaa agcggctttc
     7321 gacgctccgt gctgcctgta tggccgacac aaccaaccgg gacatgtgcc ggttgtacgc
     7381 gcaagaagaa gaacgggcgg ggaacgacgc aacggcgctt gggatttacc ttcgtttcgc
     7441 ccgcgaggac tccaataacg tcgggtcgtg ggaaaccgtg cttcgtacgg cgaaacgggt
     7501 caattcgaac gaggtgattc tgctttcgct gcggcaactt gcccgtctgg agccaaacag
     7561 cgcggaacgc tggggtgcgg tggcagatca actcggggca gagaaccgtt tcaatgaggc
     7621 atgggcactt gtccagcaga agctccgtga gttcccggaa aacccgcatc tgctgtatct
     7681 tgccgggttc tactacaacc gcgcgggaaa caagcggtcc gcactggaag ttctcgaccg
     7741 tgcggtgctg acggatgacc ccacgtggcg tggcctttca ttgaacctgc acgatagtat
     7801 cgaacccccg ttatcagaag acgagatcaa ccaggccaaa tttttcggcc gaaaagtccc
     7861 gcgaattcat cgttgcagaa ttccgggcag agaaaaacag aacgaagtaa tgcagtaagt
     7921 gtgaccgaac cgcgtgaaaa gcgggcggca gagtagttcg gtgagcgtgc gctgtacggc
     7981 gggtccgtat gggctacgct ataccatgca aacccctcgg aggcttcagc ccgaggtgag
     8041 gacaatcagg aggaacagat cgatgggtgg tcgtaaggtt ctcaccattg ccgccgtcag
     8101 cgcattgctg ttcgggatgg caagcgccca gacaggagcc ccggcacagg gcggtgcagc
     8161 gcaatcggcc gatacggcga aagtccaggt atccttcgtc gttacgcagg atggtaagga
     8221 aacggcgata ctcgagaaag ggaagcttgt cgaggtcgtc gcaacggtga agttgaacct
     8281 gccggacgcc aagaatgcga acgttcaggt tattgccgac atcgcagatc agttgaacat
     8341 cgaaaacgaa gccgacgtga aggtgaaggg cctgtacatt gacaatggca gcgggttttc
     8401 ccttcaaccg acgtcgtctg tgcgtccccg gctcggattc cttgcgcccg agggtcagac
     8461 aggtatctct gccacccagc gggtgaaatg ggttctggat ttcataaatg tcgcgccgcg
     8521 tgcgcccttt tccgtgaatg cggcagtcgt aattcctccc agtaacgaaa tcatcgg
//
LOCUS       DAOTNG010000120         8437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7574_length_8437_cov_5.538416, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000120 DAOTNG010000000
VERSION     DAOTNG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7574_length_8437_cov_5.538416"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="P5179_07205"
     CDS             complement(<1..920)
                     /locus_tag="P5179_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95040.1"
                     /translation="MTKLRLACVGAGPDKHSRSRGYLAVVKALHDLYELCAVVDPNES
                     NGREAAAEYGIPGVYTDMEKAIKETRPDVVLRLAPTDSAMAICVTAAQMGCNVISEIP
                     IDITLPRADATIKACRENNVRIEIAENVWLWPEEQLKQEIVRRGIIGRPVHARLKYPC
                     GSYHGFNGVRMILAEEAVRVLGFGGTVEVMPLPAYAGGMMTHVQWDGACVEFSSGLKL
                     LFEMPPKKPVWERNWDIIGTHGYLTGDSLVLYREGHHHGSGEEERFPIESVYREIQGQ
                     RVLQAVRVNTDPVVEWVNPYIRYGISGTDD"
     gene            complement(1053..2039)
                     /locus_tag="P5179_07210"
     CDS             complement(1053..2039)
                     /locus_tag="P5179_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRS95041.1"
                     /translation="MLDLYRWHLRARRMNNPGAIAVGFFFLFAPPTLLPAGDQVHTLT
                     GHGSPVMALACTRDSRYILSAGRGQVIQWNVRNGSRVFTLIHPGWALSIAVAPDGSQF
                     ATGSDDGLIRIWRLDNGGLLRTLEGHRGLVVALAYSDDGTTLASAGFDSVVAIWDVAG
                     TEAVRLLRGHAGEVNGVLFSATGDTVFTCSADGTTRLWNTHDGSLLQVFRGHSDSVRG
                     VAHSRRENILATCSNDSTVRLWDVKSGECVRVLRGHSDAVSAVAFSPDGRFLVSTGSD
                     RTVRVWRVDTGETERVLRGHTDWIRPLLITRDGNHIVTGASDATVMVWRRPW"
     gene            complement(2085..4025)
                     /gene="metG"
                     /locus_tag="P5179_07215"
     CDS             complement(2085..4025)
                     /gene="metG"
                     /locus_tag="P5179_07215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRS95042.1"
                     /translation="MDRFYITTPIYYVNGPPHLGHAYTTVVADALTRFARLEGKDAFF
                     LTGTDEHGEKIAQSAQSKGIAPQKWVDDVSAQFRKLWPELHIEPDDFIRTTEERHIRV
                     VQAILQKVFESGDIYYGEYGGNYCVGCERFLDDDELVDGKCPDHGVAPRFVNEANYFF
                     RMSGYQDWLISHIQENPEFIRPERYRNEILAFLRKPLKDLCISRPKNRLSWGIELPFD
                     SKFVTYVWFDALVNYVSALGWPDGERFAKYWPVAEHITAKDIIKPHGIYWPCMLKAAG
                     IPVYRHLSVHGFWRGADGRKMSKSLGNSVDPAEMAQRYGADVFRYALLREMPYGQDAN
                     IGEEIIATRQNSDLANDLGNLISRTLKLVFRSFGGVVPRVSETRVEDTALRDQWIGAL
                     TVVREAWHELRMSQGIETVIDCIRATNRYFDQMKPWELAKRGDTERLATVLVSALDSI
                     RIASVLLTPVMPERMAELRRQLGLTSSPSLEEAERWDGFAEGHRLAEGQSLFPRVDLE
                     EVRTRELHRREAATQSGTSDKPEPAPVESAAPVTIEEFRRVDLRVGTIISAEPVPGAR
                     KLLRLQVDIGEAAPRQLVAGLAEQFEPAHLLGRQVVVVANLEPAIIRGIESQGMILAA
                     DDQGRLALLGPAAAMPNGARIR"
     gene            4182..5642
                     /locus_tag="P5179_07220"
     CDS             4182..5642
                     /locus_tag="P5179_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRS95043.1"
                     /translation="MRRSAWRTTYFILIAGGLLVGASASGEAADSLRKATPAADSVSP
                     KAGLRDSLWQRLESEFPAGRMAGRGKTVAVVQVEGMIDGGLAAFIGRALDEAKAMRAA
                     AFVIEMNTYGGRVDAADEIRTALLNTDVRTITFIHNNAASAGALISYATDVIVMSPGS
                     AIGAATPVDQSGAVATNKVVSYFRSIMGETARAKGRDPRLAEAMVDSSIVVPGLENAP
                     RPLTLRTDQAIAFGVAQMQAASLAEALRYNGLEGAKMVRLETNWAEHIVRFLTHPVVS
                     GLLMTVGALGVIYELTSPGFGLPGIAGITCLVLFFGAHWIVRLAQIEELLLFAAGVAL
                     IVAELFVPGGILGIIGGALVLTGLFLSLVGRIDLVSPGDLTHAFTSIGMAVVLTFIGA
                     LVLIKSFSQLPMFRKLMLEKSQNPGTGQTASAAGPQPNLLGAVGVADSPLRPVGRVAI
                     GGKYYDAVTDGEHIENGASVKVVEVDGLRGLVVRRV"
     gene            5676..6155
                     /locus_tag="P5179_07225"
     CDS             5676..6155
                     /locus_tag="P5179_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRS95044.1"
                     /translation="MVVTWAFVIALALLGFVLLMLELFVIPGFGITGIAAIAAILGAV
                     WYANLHLGTWQAVVVFCVTLLGSVLMISRTARTGGMKQLRNPSVSPGKVWDETKAREL
                     LPQVGARGVAVTKLRPAGIVQIGDRRYDVTVLGPIAEAGQAVEVVQIEGNHIKVKAL"
     gene            6229..7254
                     /gene="floA"
                     /locus_tag="P5179_07230"
     CDS             6229..7254
                     /gene="floA"
                     /locus_tag="P5179_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703919.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRS95045.1"
                     /translation="MYDQTLLVAVGVFAVIVFLILFTYFIPVRLWIEAQFANVPVKIS
                     TLIGMRLRNVPPARIVLSLITSTKAGLNLQIDELEAHYLARGNVQLVVAALISANKAG
                     IELPFARAAAIDLAGRNVLEAVKMSITPKVIDCPDPSRGRSTIDAVAMDGIQLKAKAR
                     VTVRTNLDRLVGGAGEETIIARVGEGIVSAIGSASSHKQVLENPDVISKKVMEKGLDS
                     GTAFEILSIDIADVDVGDNIGAKLQSDQAEADKRIAQAKAEERRVMAVALEQEMKART
                     QEMQAKLVEAEAQIPLAMAEAFSKGHIGVMDYYRMKNILSDTEMRSAIAREGVSPQAG
                     DNPPARS"
     gene            7257..7883
                     /locus_tag="P5179_07235"
     CDS             7257..7883
                     /locus_tag="P5179_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95046.1"
                     /translation="MDFSWLIIFIIISIASTLLSKRKEKQEAERRAREQAGKPPKPSP
                     AAGPPPVAPGPFGPLWKPAPPKPPSRPQPVPAMATDEEDAEKERPEPQPVVPARPLFK
                     EMQRELDERAAKTASVPSQEPVFVPEDWKREAQPRPVQKVFAADIDAHEGESFSRRVV
                     ADAAPSAPVHRAKRFGIGRGDLKRAVVMAEILDKPLALRDPVPRETQF"
     gene            8019..8102
                     /locus_tag="P5179_07240"
     tRNA            8019..8102
                     /locus_tag="P5179_07240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8053..8055,aa:Leu,seq:caa)
BASE COUNT         1737 a         2453 c         2495 g         1752 t
ORIGIN      
        1 atatcgtccg ttccggagat gccgtagcga atgtagggat tgacccattc aacgaccggg
       61 tccgtattca cccggaccgc ctgaagcaca cgttgcccct gaatctcgcg atataccgat
      121 tcgatgggga aacgctcttc ttcgcccgaa ccgtggtgat gtccttcgcg atacaacacc
      181 agtgaatcac cggtcaagta gccatgcgtg ccgatgatat cccagttgcg ttcccagaca
      241 ggctttttgg gcggcatttc gaacaaaagc ttgagcccgc tggaaaactc cacacaggcg
      301 ccgtcccact ggacgtgcgt catcatgccg cctgcgtacg caggaagcgg catcacctcc
      361 accgttccac cgaacccgag cacgcgaact gcttcctcag ccaggatcat tcgaacgcca
      421 ttgaagccgt ggtagcttcc acagggatac ttcaggcgtg cgtgaaccgg tcttccgata
      481 attcccctcc gcacgatttc ctgtttcagc tgctcttccg gccagagcca cacgttctcc
      541 gcgatctcga tcctgacgtt attctcgcgg caggccttga tggtagcatc cgcacgaggg
      601 agcgtgatat caatcggaat ttcgcttatg acgttgcaac ccatctgcgc agccgttacg
      661 caaatcgcca tggcagaatc tgtcggcgca aggcggagca ccacatcggg tcttgtctcc
      721 ttaattgcct tttccatatc tgtgtacacg ccgggaatac cgtattctgc agccgcttcc
      781 cggccgttcg attcgtttgg atccacgact gcgcacagct cgtacagatc gtgcagagcc
      841 ttcacgacgg cgaggtagcc tctgctccgg gagtgtttgt cggggcccgc gccgacacat
      901 gccagacgaa gctttgtcac ggttcttccc tccaagaagt ctggttcccg ggataacccc
      961 gggtaccgtg tgccacggta agtttccttc agtctgtgct tccgttgact aatgtgagca
     1021 ctcggcggtg cagaacaccg tacgtctccc cgttaccagg ggcgccgcca taccatcacg
     1081 gtggcgtcac tcgcgcccgt tacgatgtgg ttgccgtcac gcgtaatcag cagcgggcgg
     1141 atccaatcgg tatgtcctct caacactcgc tccgtctcgc cggtgtccac ccgccagacg
     1201 cgcacagtcc ggtccgaacc cgtcgaaacg agaaaccgcc cgtccggact gaacgcaacc
     1261 gcggatacgg catcggaatg cccacgcagg acgcgcacac attctccgct cttgacgtcc
     1321 cacagccgca ccgtggagtc gttgctgcac gttgcaagga tgttctcgcg ccgcgagtga
     1381 gccacccccc ggactgaatc ggaatgcccg cgaaacacct gaagcaggga cccgtcgtgc
     1441 gtgttccaca gacgagtggt gccgtctgcg gaacacgtga agacggtgtc gcccgttgcg
     1501 ctgaaaagaa cgccattcac ctcccctgcg tgaccccgga ggagccgaac ggcttcggtg
     1561 ccggcaacgt cccagatggc aaccaccgaa tcaaaacctg cggacgcaag ggttgtcccg
     1621 tcatcgctgt acgccagcgc caccacaagg cccctgtgtc cctccagagt ccgcaacaaa
     1681 ccaccattgt ccagccgcca gatacgaatg aggccgtcat cggacccagt tgcgaactgt
     1741 gagccatcgg gcgcgacggc aatgctgaga gcccaccccg ggtgaatgag ggtgaacacc
     1801 cgcgacccgt tgcgcacgtt ccactgaata acctgccctc tgcctgccga aaggatgtat
     1861 ctgctgtccc tcgtgcatgc gagtgccatt acggggctgc catggccggt gagagtgtgg
     1921 acctggtcac cggcaggcag gagtgtcggt ggagcaaaga gaaaaaagaa ccccaccgca
     1981 atggcacccg ggttattcat ccgtcgagca cggaggtgcc accgatacag atccagcaac
     2041 gcagcactac cttcccactt tgttctgaac gagttcctcg ggtgttaacg gatccttgcg
     2101 ccgtttggca tggcagcagc agggccaaga agtgccagac ggccctgatc gtccgcggcg
     2161 agaatcattc cctgagattc tataccccgg ataattgccg gttcaagatt cgcaacgacg
     2221 acgacctgcc tcccgaggag gtgagccggt tcgaattgct cggccagccc tgcgacgagt
     2281 tgtctgggag cagcttcacc tatgtccacc tggagtcgga gaagcttccg ggcccccggg
     2341 acgggttctg cggaaatgat ggtgcccaca cgaaggtcaa cccggcggaa ttcctcgatg
     2401 gttactggtg cagcgctttc cacaggcgcc ggctcgggtt tgtcgctggt tccagattga
     2461 gtcgcggcct cacggcgatg gagctcccgc gtgcgaacct cttcgaggtc aactcgggga
     2521 aaaagggatt gtccttccgc aagccggtgg ccttcggcga acccgtccca gcgttctgcc
     2581 tcttccagag aaggcgatga ggtaaggcca agctgcctgc ggagttccgc catgcgttct
     2641 ggcatcacgg gagtcagcag cacggacgcg atccggatgg agtcgagggc ggataccaat
     2701 actgtcgcca aacgttccgt atcaccgcgc tttgcaagct cccacggctt catctggtca
     2761 aaatatcggt ttgttgctcg tatgcaatcg attacggttt ctatcccctg cgacatacga
     2821 agttcatgcc acgcctcgcg cacgaccgtc agcgcaccga tccattggtc acgaagcgcg
     2881 gtatcttcaa cgcgcgtttc cgacacacgc ggcacgacgc cgccgaaaga acggaacacc
     2941 agcttcagcg tccgcgagat caggttgccc aaatcgtttg ccagatcact gttctgccgc
     3001 gtcgcgatga tttcctcacc gatattcgca tcttgcccgt agggcatttc ccgcaacaac
     3061 gcgtaccgaa aaacgtccgc gccgtaccgt tgcgccattt ccgcaggatc aacagaattg
     3121 cccagagact tggacatttt ccgcccgtca gcgccccgcc agaaaccgtg cacgctgagg
     3181 tggcggtaca caggaatgcc tgcagctttg agcatgcacg gccagtagat cccgtgtggt
     3241 ttgatgatat ccttggccgt gatgtgttcg gctaccggcc agtatttcgc gaaccgctcc
     3301 ccgtccggcc accccagcgc tgatacgtaa ttcaccagcg cgtcgaacca cacgtacgtg
     3361 acgaacttgc tgtcaaaagg cagctcaatt ccccaggaaa ggcggttttt cggccggctt
     3421 atgcaaagat ctttcagcgg tttcctgagg aacgcgagaa tctcgttgcg atagcgctcc
     3481 gggcggatga actccgggtt ttcttgaatg tgggagatca accagtcctg atacccgctc
     3541 atgcgaaaga agtaattcgc ttcgttcacg aagcgcggcg caacgccgtg atcagggcat
     3601 ttgccgtcga ccagttcgtc atcatcgaga aaccgctcgc aaccgacgca atagttgccc
     3661 ccgtactcgc cgtaatagat gtccccggat tcaaacactt tctggaggat cgcttgcacc
     3721 acgcgtatgt ggcgctcctc cgttgtgcgg atgaaatcgt cgggttctat gtggagttca
     3781 ggccagagtt tgcggaactg ggcgctgacg tcgtctaccc atttttgcgg cgcgattcct
     3841 ttggattgcg cgctctgcgc gatcttctcg ccgtgctcat ctgtgccggt aaggaagaac
     3901 gcgtccttac cctcaagacg agcaaaacga gtgagtgcat cggctacaac ggtggtgtac
     3961 gcatggccga ggtgcggcgg gccgttcacg taataaatcg gggttgtgat gtagaatctg
     4021 tccacggaca acgcatcctt tccttctgtg tttccccttt taacgaaaca cacgcaggcg
     4081 ccaaaaagcc aacgggcgcg catattgttg acctattccg gtgtttgtcg taagatgtat
     4141 tgttacatgt tgtaacgaga ctcccgttgg gggtgcagaa tatgcggcgt tccgcgtggc
     4201 gcaccactta ttttatcctg attgcaggcg gtttgcttgt tggcgcaagt gcgagcggcg
     4261 aagctgccga ttcgttgcgg aaagcaaccc cggcggctga ttccgtttcc ccgaaagccg
     4321 gtcttcgcga cagcctctgg cagcgtctcg aatccgagtt ccctgcaggg cgcatggcgg
     4381 gcagggggaa gacagtcgcg gttgttcagg tcgaggggat gatcgatggg ggccttgccg
     4441 cattcatcgg gcgcgcgctt gatgaagcga aagcaatgcg cgccgcggcg tttgtgatag
     4501 agatgaacac ctacggggga cgtgttgacg ccgccgacga aatcagaacg gcactcctga
     4561 ataccgacgt ccgaacaatc acgttcattc acaacaacgc ggcatccgcg ggagctctca
     4621 tttcctacgc gacagatgtc attgtaatgt cgccgggaag tgctattggc gcggccacgc
     4681 cagtagatca gagtggcgcc gttgcgacca acaaggttgt ttcctatttc cgtagcatca
     4741 tgggcgaaac agcgcgcgcg aagggccgcg acccgcgtct cgcggaagcc atggtggatt
     4801 cctcgatcgt tgtccctggc ctggaaaacg cgccaagacc gctcacgttg cgcaccgacc
     4861 aggcgatcgc gttcggtgtt gcccagatgc aggctgcctc gcttgcggag gccctccgct
     4921 acaacggtct cgagggcgcc aaaatggttc gcctcgaaac gaactgggcg gaacacatcg
     4981 tccggttcct cacgcatcct gtggtgagtg gccttctcat gacggtgggc gcccttggag
     5041 tgatttacga actcacgagc ccggggttcg gtcttcccgg cattgccggc ataacgtgcc
     5101 tggtgctctt tttcggggca cactggatag taaggctcgc acagattgag gagcttctgc
     5161 tttttgcggc gggagttgcg ttgattgtgg cggagttgtt cgtgccgggt ggaatactcg
     5221 gaatcatcgg cggtgcgctc gtgctgaccg gtctgttcct cagtctggtg ggcagaatcg
     5281 atctcgtgag ccccggcgat ctgacgcatg cgttcacttc tatcggcatg gccgtcgtgc
     5341 tgacgttcat cggtgcgctg gtcttgatca agtccttttc acaattgcca atgttccgaa
     5401 agttgatgct ggaaaagagc cagaacccgg gaactggtca gaccgcttcg gcagcggggc
     5461 cacaaccgaa ccttctcggt gccgtgggcg ttgccgattc gccgcttcgt ccggtagggc
     5521 gcgttgcgat cggcggcaag tactacgatg cagttactga cggcgagcat attgaaaacg
     5581 gtgcgtccgt caaagtagtg gaagtggatg gtctccgggg tcttgttgtt cgaagggtgt
     5641 aattgtcagc ctcgcggaag gcggaaggcg cggaaatggt tgttacgtgg gcatttgtga
     5701 tagctcttgc gctcctcggc tttgtgttgc tcatgcttga gctgttcgtg attccggggt
     5761 tcggaatcac ggggatcgcc gcgattgccg caattctcgg ggcggtatgg tacgcgaatc
     5821 tccatctcgg aacatggcag gcggtggtgg ttttctgcgt gacattgttg ggatcggtac
     5881 tgatgatttc ccgcaccgcc cggacgggtg gcatgaagca actccgaaat ccgtccgttt
     5941 ctcccggcaa ggtgtgggat gaaaccaaag cgcgcgaatt actgccgcag gtcggtgcgc
     6001 ggggagtggc cgtaacgaag ttgcggccgg cggggatcgt gcagataggg gacagaaggt
     6061 acgacgtaac agtcctgggc cccatcgccg aggcgggaca agcagtggag gttgttcaga
     6121 tagaagggaa ccacataaag gtgaaagccc tgtgagtttt cccgttaacg gtttcgaaaa
     6181 gtgccagcgg cgcggagtca acgccgcgta gaccagaaag ggattgcaat gtacgatcag
     6241 acgctccttg tggcggtcgg cgtgttcgca gtaatcgttt tcctcatcct gttcacgtat
     6301 ttcattccgg tgcggctgtg gatcgaagcg cagtttgcga atgttccggt caagatttcc
     6361 acgctcatcg gcatgcgtct gcggaatgtg cctcccgcgc gaattgtcct ttcgctgatc
     6421 acttcgacga aagccgggct caatctgcag atcgacgaac tggaagcgca ttatctggcg
     6481 cgaggaaatg tccaacttgt ggtggccgca ctgatttcgg cgaacaaggc cggcatcgag
     6541 cttccatttg cacgcgctgc cgcgatcgat cttgcaggca ggaacgtgct cgaagcagtc
     6601 aagatgagca tcacaccaaa ggtgatcgat tgtcctgatc cctcccgcgg ccggtccaca
     6661 atcgatgccg tggcgatgga tggcattcag ctcaaagcaa aggcccgcgt cacggtgcgc
     6721 accaacctcg atcggctcgt tggtggcgcg ggagaagaga cgattatcgc ccgtgttggc
     6781 gaaggaatcg tgagcgcaat tggttcggca tccagccaca aacaggtgct tgagaacccg
     6841 gatgtgattt ccaagaaggt gatggagaag gggctggatt ctggaaccgc ctttgagatt
     6901 ctttccatcg atatcgcgga cgtggatgtc ggggacaaca tcggcgcgaa attgcagagc
     6961 gatcaggcag aagctgacaa gcggattgcg caggcgaaag ccgaggagcg ccgggtgatg
     7021 gcggttgcgc tggaacagga gatgaaagcc agaacacagg aaatgcaggc gaagctggtg
     7081 gaagctgaag cccagattcc tctggcgatg gcggaagcgt tcagcaaggg acacatcggt
     7141 gtcatggact actacaggat gaaaaacatt ctctcggaca ccgagatgag atccgccatt
     7201 gcgcgcgaag gcgtttcccc gcaagccggg gataacccgc cggcaaggag ctgagcgtgg
     7261 atttctcgtg gctgatcatc ttcatcatca tcagcattgc ctccacgctc ctgagcaaac
     7321 gaaaggaaaa acaggaggct gaacggcgcg cgcgggaaca ggcggggaag cccccgaaac
     7381 catcgcctgc ggccggtccg cctcccgtgg ctccggggcc tttcggccct ctctggaaac
     7441 ccgcgccacc gaaaccgcct tctcgaccgc aaccggtccc tgcaatggcc acggacgaag
     7501 aggacgccga gaaggagcgc ccggagccac agccagtggt cccggcacgc ccccttttca
     7561 aggagatgca gcgggaactg gacgaacgtg cggcgaaaac tgcttcggta ccgagtcagg
     7621 agccggtgtt tgtgccggaa gattggaaac gtgaggctca accacggccg gttcagaagg
     7681 tgtttgcagc cgatatcgac gcgcacgaag gagagagttt ctcgcggcgt gtcgttgcgg
     7741 atgctgcgcc atccgctccc gtccaccgcg caaaacgctt tggaatcggc cgcggggacc
     7801 tcaagcgagc agtcgttatg gcggaaatac tggacaaacc cctcgcgctg cgggacccag
     7861 taccgcgtga gacgcaattt tgatctgttt gcgcccggga attgacaatc ccgagacggt
     7921 tgaccgtcga aagggcgtgt gactacctta atatttcgcc atcccggcat cttttccggg
     7981 gcaaagggaa aggcggggaa ggcagcgacc gttcagaagc cgaagtggtg gaattggtag
     8041 acgcgccgga ctcaaaatcc ggtgtccgcg aggacgtgtg ggttcgattc ccaccttcgg
     8101 cattgcagta aaaacaggga cttacgggat cacgtgagtc cctgttttcg ttcccctata
     8161 acctccatgg cacactcatg gcacaatttg aaaaaagccc tgcgaaaccc ctcaagtgtg
     8221 ccaaaacgtg ccatcgtgtt cggtgattga tcgtaacatc attatttaca acgtcaagtc
     8281 gcgcaccgta cacattcttt ccggaaggtg ttggtttcgt cgccgagttg ccgtactctc
     8341 ccgtaacaaa aggtctctgc gtcgaacagc gaagaccgcc gcgcaaggcg gtcgacaaag
     8401 ggtttccccg tggccacgat ccaagcgcga attactc
//
LOCUS       DAOTNG010000121         8376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7639_length_8376_cov_5.364379, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000121 DAOTNG010000000
VERSION     DAOTNG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7639_length_8376_cov_5.364379"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="P5179_07245"
     CDS             complement(<1..1264)
                     /locus_tag="P5179_07245"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRS95047.1"
                     /translation="METTITTYALILALPLVAFVVQVFFGKRLPRGGDWLVIGAICID
                     LLLSLRVFFGALFFSSFASTPFVWNLRGWLGDAGTGVLALSTGIMVDNVTAMMLVVVT
                     GCSTLIFLFSTGYMAGHRRYSLFFAYLSLFAFSMLLLVLGNNLIALFMGWELVGLSSY
                     LLIGFLFEEPVVAPDIVPASACKKAFITNRIGDTGFLLGILTLFSFTDKIAYGDVFEA
                     VRNGAFGDHTTLLTVAGILLFCGAMAKSAQFPLHVWLPDAMAGPTPVSALIHAATMVA
                     AGVYMVARLYPLFTPDALTFIAYVGAFTALFGATIALVMNDIKRVLAYSTISQLGYMV
                     FGLGVGAYSAGLFHLMTHAFFKALLFLAAGSVIHAMHRAYHHAGLEWNPRSRENDAQD
                     MRNMGGLRKAMPVTVATTAVAVLAISGVP"
     gene            complement(1270..1575)
                     /gene="nuoK"
                     /locus_tag="P5179_07250"
     CDS             complement(1270..1575)
                     /gene="nuoK"
                     /locus_tag="P5179_07250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRS95048.1"
                     /translation="MVQLPHFLTVSALLFAAGVFAVVTRRNAVALLMGVELMLNAANL
                     NFAAFSRFSHNSLDGQIFAMLSIVTAAAEVAVALAIVLNIYRNIETIDADQAGDLRG"
     gene            complement(1577..2092)
                     /locus_tag="P5179_07255"
     CDS             complement(1577..2092)
                     /locus_tag="P5179_07255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRS95049.1"
                     /translation="MNAYDLAFYLLAAMALVGAAITVLSRRLVYAAFSLLLAFLGVAG
                     LYALLAADFLAIAQVVVYVGGILVLLLFGVMLTHRIYDTTLKSVRVQPLLGGLVAAGI
                     AILLISVAWNTPWNTTHEIAAEPTTAKIGEAFMKHYVFPFEFVSLILLVALIGAALIG
                     RSRTPGSGNGR"
     gene            complement(2089..2787)
                     /locus_tag="P5179_07260"
     CDS             complement(2089..2787)
                     /locus_tag="P5179_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS95050.1"
                     /translation="MRAFFGYWRGIASGFMTTLVGMVAAIRHVGRKEYTLEYPERRDV
                     LPPRARMSLFMNADECIACAQCARACPVNCITVASVKRPKDEEPPLTVVGQRKKVLVL
                     TEYRIDMGRCCFCGLCVEPCPTHSLYMTPDFEMAIVQEAPEPDEVLREGESFAAADAR
                     RARSQIQAQHPRFGRTGLVYDFLAITDDLRRRGETVPVNPLFPEEHAQLVQNRVRPPN
                     QDAAGTTDPPGGRN"
     gene            complement(2784..3950)
                     /gene="nuoH"
                     /locus_tag="P5179_07265"
     CDS             complement(2784..3950)
                     /gene="nuoH"
                     /locus_tag="P5179_07265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRS95051.1"
                     /translation="MQEMYETLLAALGAERVIPFSVGFAVLAVVFALFAAIMGAVVAL
                     IMVLAERKISADIQERIGPNRVGPMGFFQTVADAVKLLTKENVLPRKADSILHYCAPT
                     LVFTGALLPVAAIAWSNRIVAADLNIGVLYVVGTASFGVIGRLMAGWASNNKWSLLGG
                     MRAAGQMISYEIPNSIAILSVIVLAGTLNLQELSEAQMGGIHQWFVFRSPFAFVACLI
                     YLVSSLAEVNRTPFDLPEAESELVSGYHTEYSGIRWAIFMMSEYIGMVVVNAVAVALF
                     FGGWNGGLPGEPLLGGWIWYLLKIAVLMYCQVWIRFVLPRLRIDQLMSLCWKNLVPLG
                     FLALLGASVQALLGPTGQRVMMVAAWLVFGGFVTLLFVAVRKTRHVPAQGGFSG"
     gene            complement(3970..5085)
                     /locus_tag="P5179_07270"
     CDS             complement(3970..5085)
                     /locus_tag="P5179_07270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRS95052.1"
                     /translation="MPLKTEEMILNVGPQHPSTHGVLRLEVTTDGELVTSAEPTIGYL
                     HRCFEKYAETLTYQQIIPYTDRLDYLSSMNNNFGFVVAVEKLMGIHVCERAERIRVIM
                     AELNRIASHLMFIGSFSNDLGAMTPFLWCFRDREEILKIFEEVSGARLLYNYYRIGGL
                     AADLTPGIEEKIYSFLNQFEAHMLELDDLFTRNAIVIARLKGVGMIPREMAIAYGLSG
                     PMLRASGVDWDCRRDEPYSIYASLRFEVPVGGEKLGALGDCYARYLVRRAEIDQSVSL
                     VRQCLADIPPGDVKEQVKRNVRPPVGEVYVRTENPRGEIGFYLRSDGSTKPSRVKVRA
                     PSFCNMSVFSRISKDILVADMIAILGSVDIVLGEIDR"
     gene            complement(5110..5676)
                     /locus_tag="P5179_07275"
     CDS             complement(5110..5676)
                     /locus_tag="P5179_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRS95053.1"
                     /translation="MDALAIATMVKDVFGGAVLAVDQPEVEPVAVIEPAKVAEVARFL
                     SETPELAFDYLMCLSGFDTMPTEEDHPARNTPEEDTGAPSTETAAPPLGVAYHLFSYS
                     HRHRFAFKAHTPRENPHIPSVAEIWPAADWHEREAFDLFGIIFDGHPDLRRILLPEDW
                     VGHPLRKDYAAQESYRIGDACVQIARGW"
     gene            complement(5684..6175)
                     /locus_tag="P5179_07280"
     CDS             complement(5684..6175)
                     /locus_tag="P5179_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRS95054.1"
                     /translation="MGMLEERFDPNIVTTTVDAFLDWARKSSLWPMGFGLACCAIEMM
                     ATSMSRYDLDRFGASVFRSSPRQCDLMIVSGTVTLKMASRVKRLYDQMAEPRYVISMG
                     SCANTGGPYWEHGYHVLKGVDRIIPVDVYIAGCPPRPEALIDGVLQLQKKITEQRVVR
                     KAV"
     gene            complement(6258..6659)
                     /locus_tag="P5179_07285"
     CDS             complement(6258..6659)
                     /locus_tag="P5179_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRS95055.1"
                     /translation="MYLHFGAVLAFLLFSAVFVAITYGISALFRPANPSAEKLATYEC
                     GEIPFGGSWIQFNLRYYLIALVFLIFEVEVVFLYPWAVVLRTIGAFAFWEMIVFLAIL
                     LVGFAYVWRKGDLSWFTPSGSGHTDTTFSGK"
     gene            complement(6820..7785)
                     /locus_tag="P5179_07290"
     CDS             complement(6820..7785)
                     /locus_tag="P5179_07290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95056.1"
                     /translation="MNDDFVLKTGENLECRVIDRRNTDLVPALRTLLAHKGDIWQFHL
                     EEEFAGRIKDLETRFYVGLIGSDLVGNIMTVEFKGLGIMGHVYTPPEHRRKGICDVLM
                     DFHMADFRERKGNALYLNTGFESAPFFIYQKHGYRPIPEAPGSMWWSPDSWSPDTLWD
                     DLERLKIAEPAWRHWPSANVFMLQTCLPAVRNVTYGKFGCANAEATFLHVLSDLRNPE
                     KRVQARVLETRDGHMAGLATLAPERRWGSLGTTYVFDLCVHPNAEDVPEQLAEDFQWP
                     AAHVLAYAADDDTAAVNRYAQMGFRPHSHIERFFGTTGLLILSRE"
     gene            complement(7782..>8376)
                     /locus_tag="P5179_07295"
     CDS             complement(7782..>8376)
                     /locus_tag="P5179_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRS95057.1"
                     /translation="FSKYSDAVLPLTGLLRHPDWKTRQVAAESLWRLKDTRSEPALVS
                     LLEDSVALVRHAAALALGTCGTSRSDTALVRRLSDESQIVRYAAEQALGPRATARPLL
                     LQIAGSQGTFAAMHALRALRVDTSDTSYAPVLRSIIRSDGHWAVRAEAANSISALRMQ
                     SLSSDLQEVRMDTHHPFLAQRIEEAITTLNGANRRNE"
BASE COUNT         1882 a         2491 c         2386 g         1617 t
ORIGIN      
        1 acggcactcc agaaattgcc agcacggcaa cggcagtggt ggcaaccgtg acaggcatgg
       61 cctttcgcaa accacccatg ttgcgcatgt cctgggcgtc attctcacgg gatcgcgggt
      121 tccattcaag ccccgcatgg tggtacgcgc gatgcatcgc atggatcacg cttcctgcgg
      181 caaggaagag cagagctttg aagaacgcgt gggtcatcag gtggaaaagg cccgccgagt
      241 acgcacctac gcccagaccg aacaccatgt atcccagctg gctgatggtg ctgtaggcga
      301 gcacccgctt gatatcgttc atcaccagcg cgatggtggc gccgaacaac gcagtgaacg
      361 cgcctacgta cgcgatgaag gtcagggcat ccggcgtgaa aagcgggtac aaccgggcaa
      421 ccatgtatac acccgccgct accatcgttg cggcgtgaat cagagcactc accggcgtcg
      481 ggcctgccat cgcgtcggga agccacacgt gcaacgggaa ctgggcggat ttcgccatcg
      541 cgccgcagaa caagagaatc ccggcaacgg tcaggagggt cgtgtggtca ccgaaggcgc
      601 cgttccgaac cgcttcgaag acgtcaccgt acgcgatttt gtcggtgaaa gagaacaggg
      661 tcaggattcc gagcagaaaa cctgtatcac cgatgcggtt cgtgataaat gcttttttgc
      721 aggcggaggc tggaacgata tcgggggcta ccaccggctc ctcgaacagg aagcctatga
      781 gcaggtatga actcaagccg acgagttccc accccatgaa cagcgcgatc agattgttgc
      841 ccaaaaccag caggagcatg gaaaacgcga acagggacag atatgcgaag aacaggctgt
      901 agcgacggtg acccgccatg taccctgtgc tgaacaggaa aatgagggtg ctgcaacccg
      961 tcacaaccac aagcatcatc gcggtgacat tatccaccat gatgccggtt gacaacgcga
     1021 gaacccccgt ccccgcgtcg ccaagccagc cgcgcaggtt ccacacaaac ggtgtgctgg
     1081 cgaatgagga aaagaacagc gctccgaaga agactcgcag ggacagcaac aggtcgatac
     1141 agatcgcgcc gattaccaac cagtctcccc ctcgagggag cctttttccg aaaaacacct
     1201 gcacaacaaa cgcgacaagc gggagcgcga ggatgagcgc atacgtcgtg atggttgttt
     1261 ccacgagcct tatcccctga gatcgcccgc ctggtcggcg tcaatggttt cgatgttgcg
     1321 gtaaatgttc agtacgatcg caagtgctac tgcaacctct gccgcagccg taacgatgct
     1381 cagcatcgca aaaatctgtc cgtccagcga attgtggctg aaccgcgaga atgcggcgaa
     1441 attcaagttc gcggcgttca gcatcaattc cacacccata aggagcgcga ctgcgtttcg
     1501 tcgcgtcact actgcgaaca cgcccgccgc aaaaaggagc gcgctaacgg tgagaaaatg
     1561 aggaagttgt accatatcaa cgtccgttgc ctgaaccggg tgtgcggcta cgtccgatca
     1621 gagcagcacc gatgagggct acgagcagga tcagcgacac gaactcgaac gggaacacgt
     1681 aatgcttcat gaacgcttcg ccgatttttg ccgtcgtcgg ttccgcggcg atctcgtgtg
     1741 ttgtgttcca gggcgtattc catgccacac tgataagcag gatggctatc cctgcggcga
     1801 cgaggccgcc gaggagcggc tgaacgcgca cgctcttcag ggtcgtgtcg taaattcgat
     1861 gcgtcagcat aaccccgaag agaagcaaca cgagaattcc gcccacgtag actaccacct
     1921 gtgcgattgc aaggaaatcg gccgccagaa gtgcgtagag acctgcaacg ccgagaaagg
     1981 cgagcaaaag gctgaatgcg gcgtacacaa ggcgacggct caaaacggta atggccgcgc
     2041 caaccagcgc catcgctgcg agcaagtaga aggcgaggtc gtacgcgttc aattgcggcc
     2101 tccaggggga tccgtggtac cagcggcgtc ctggtttggc ggccggaccc ggttttgtac
     2161 caactgagcg tgctcttcgg ggaaaagcgg attcacaggt accgtttcgc ctcgacgccg
     2221 cagatcgtca gtgatcgcca gaaaatcgta cacgagcccc gtacggccaa acctcggatg
     2281 ctgcgcttgt atttgcgacc gcgcccgtcg cgcgtctgcg gcggcgaaac tctccccctc
     2341 ccggaggact tcatcgggtt ccggggcttc ctgcacaatc gccatttcga agtccggagt
     2401 catgtacagg ctgtgcgtcg ggcagggttc gacgcacaga ccgcagaaac aacaccttcc
     2461 catgtcaatt ctgtattccg tgagcaccag caccttttta cgttgtccga caaccgtgag
     2521 gggcggttcc tcatccttgg ggcgtttgac cgaagctacc gttatgcagt ttacggggca
     2581 cgcgcgggca cactgagcgc acgcgatgca ttcgtccgcg ttcatgaaca gcgacatccg
     2641 ggcccgggga ggaagtacgt cgcgacgttc tgggtattcc agcgtgtatt ccttccggcc
     2701 gacgtgcctg atggctgcca ccatgccgac cagtgtcgtc atgaaaccgg aggcaatgcc
     2761 tcgccagtac ccgaagaagg ccctcatccg gaaaatccac cctgcgcagg aacgtgtcgt
     2821 gtcttgcgga ccgccacgaa caggagtgtt acaaatccgc caaacacgag ccacgcagca
     2881 accatcatca cgcgctggcc cgtggggccc agaagcgcct gcaccgatgc gcccagcaac
     2941 gcaaggaaac cgagaggaac gaggtttttc cagcacagtg acatgagctg gtctatgcga
     3001 agtctcggca gcacaaacct tatccagacc tggcagtaca tcagaacggc gattttgagc
     3061 agataccaga tccagccgcc cagcaggggt tctcccggca atccgccgtt ccatccgccg
     3121 aagaacaaag cgacggcaac agcattcaca accaccatgc cgatgtactc cgacatcatg
     3181 aaaattgccc agcgaattcc ggaatactcg gtgtggtagc cggatacaag ctccgattca
     3241 gcctccggca gatcaaacgg tgtacggttc acttcggcca gtgaactcac gaggtaaatg
     3301 agacaggcga cgaacgcgaa cggcgagcgg aacacaaacc actggtgaat cccgcccatc
     3361 tgagcctctg agagttcctg cagattcagt gttcccgcca gaacgatcac cgaaaggatg
     3421 gcgatgctgt tgggtatctc gtacgagatc atttgtccgg cggcgcgcat cccgccgagc
     3481 aacgaccatt tgttgtttga ggcccacccg gccatgagcc tgccaatcac cccaaagctc
     3541 gctgtcccaa cgacatacag gacgccgatg ttcaggtcag cggccacaat gcggttgctc
     3601 cacgcgatgg cggccaccgg tagaagcgcg cctgtgaaca caagcgttgg tgcgcaatag
     3661 tggaggatac tgtcggcttt tcgtggaagg acgttttcct tcgtgagaag cttgaccgca
     3721 tcggctaccg tctggaagaa acccatcggg cccacgcgat tcggtcctat tcgttcctgg
     3781 atatccgcag aaatcttccg ttcggcgaga accattatga gtgccactac agcacccatg
     3841 atcgccgcga acaatgcgaa aacgaccgcc agcacggcaa atccgaccga aaaagggatg
     3901 accctctcgg caccaagcgc ggcgaggagt gtttcgtaca tctcctgcat cagtgttttc
     3961 cgttgtgcgt catctgtcga tttcgccgag aacgatatca acgctcccga gaatggcgat
     4021 catgtccgct accaggatgt ctttggaaat gcggctgaac acactcatgt tgcagaagct
     4081 cggggcacga actttcacac gcgagggttt cgtcgatccg tcacttcgca ggtagaaacc
     4141 gatctctccc cgcggatttt cggttctcac gtaaacctcg ccgacaggtg ggcggacgtt
     4201 tcgtttcacc tgttctttga cgtctcccgg cggaatatcg gcaaggcatt gccgtacgag
     4261 tgatacagat tggtcgatct cggcccggcg gaccagatac cgcgcgtaac aatcgcccaa
     4321 agcgccgagt ttttcgcctc ctaccgggac ttcaaaacga agcgacgcgt aaatcgaata
     4381 cggttcgtca cgccggcaat cccaatctac cccgctggcg cggagcatcg ggccggaaag
     4441 cccgtacgcg atggccatct cgcggggaat catccccaca cccttgagac gggctatcac
     4501 gatggcgttg cgcgtgaaaa ggtcgtccag ctcgagcatg tgcgcttcga actggttgag
     4561 aaacgagtaa atcttctctt cgatgccggg ggtcaggtcg gccgcaaggc cgcctatccg
     4621 ataatagttg taaagaagcc gcgcgcccga tacttcctcg aagatcttga ggatctcctc
     4681 cctgtcgcgg aagcaccaca gaaaaggcgt catggctcca agatcattgc tgaagcttcc
     4741 gatgaacatc aagtggctgg cgatgcggtt gagctctgcc atgatcaccc gaatccgctc
     4801 cgcccgttcg cacacgtgaa tccccataag tttttctacg gcaacgacaa aaccgaaatt
     4861 gttgttcatc gacgagaggt agtcaagccg gtcagtgtag gggattattt gttggtaggt
     4921 gagtgtctcg gcgtatttct caaaacagcg gtggaggtac ccgatggtcg gctcagcact
     4981 ggtgacaagc tcgccgtcgg tggttacctc cagccgaagc acaccgtgcg tcgatgggtg
     5041 ctgaggcccc acattgagaa tcatttcctc tgttttgagc ggcatagtct tccggctgtt
     5101 ttccaggggt taccaacccc gcgcaatttg cacgcaggcg tccccgatcc tgtagctttc
     5161 ctgcgctgca tagtcttttc gcagaggatg cccgacccag tcctcgggaa gcaggattct
     5221 gcgcaggtct ggatgcccat cgaagatgat tccgaaaagg tcgaacgctt cacgttcgtg
     5281 ccagtcggca gcaggccata tctccgcaac actgggaatg tggggatttt cgcgcggcgt
     5341 gtgcgctttg aacgcgaacc ggtggcggtg ggaataggaa aagagatggt acgccacgcc
     5401 aaggggaggt gccgccgttt cggtggaggg tgctccggta tcctcttccg gcgtgttccg
     5461 ggctggatga tcctcctcag tcggcatcgt atcgaagccg ctgaggcaca tcagatagtc
     5521 gaacgcgagt tccggtgttt cgctgaggaa acgagcaacc tccgccactt tcgcgggttc
     5581 gatcactgct acaggctcga cctccggctg gtcaacggca agaaccgcgc cgccaaagac
     5641 gtccttcacc atcgttgcga ttgccaacgc gtccaagtct gcctcacacc gccttccgga
     5701 ccacgcgctg ttccgtgatt tttttctgga gctgcagcac gccatcaatc aatgcctcgg
     5761 ggcgaggagg acaccccgca atgtacacat ccacgggaat gatgcgatcc acgcctttca
     5821 ggacatggta cccgtgctcc cagtatggtc cgccggtgtt cgcgcaactg cccatggaaa
     5881 tcacgtatct gggctcggcc atctggtcgt aaagacgctt gactcgcgag gccattttca
     5941 gggtcaccgt tcccgacacg atcatgagat cgcactgcct gggtgaggac cgaaacactg
     6001 atgctccgaa ccgatcgagg tcataccggc tcatactggt agccatcatc tcgatcgcgc
     6061 agcacgccaa cccgaatccc atcggccaca acgaagactt gcgtgcccag tccaggaagg
     6121 cgtccacggt cgtggtgacg atgttcggat cgaatctctc ttccagcatg cccatcggga
     6181 gatcccttac accttttcga actgctcggt ttccacacat atcatccgat gacagcctgc
     6241 agaacgtgtc acagtattca tttccccgaa aacgtggtgt ccgtgtgtcc tgaaccagag
     6301 ggagtgaacc aggaaaggtc gcctttccgc cagacgtacg cgaagccgac aagaaggatg
     6361 gcgaggaaaa cgatcatctc ccagaacgcg aatgcaccta tggtccgcaa cacgaccgcc
     6421 cacgggtaca gaaacaccac ctccacctcg aagatgagga acaccagggc gataagatag
     6481 taccggagat tgaactggat ccacgaaccc ccgaaaggga tttcgccgca ttcgtacgta
     6541 gcgagctttt ccgcggacgg gttcgcgggg cgaaaaagtg cgctgatgcc ataggtgatt
     6601 gccacgaaca ccgcactgaa cagcagaaaa gcaagaacag caccgaaatg gaggtacatg
     6661 attactccag ggagatgcgg atcgcgctga gggaatgtgc aaacggtgcc acaaacgcaa
     6721 cttctgtatg caagaaagta gggaaggcgt atcgctgacg caaggattcc cgggtttcca
     6781 cctgctcgcg ttgggactgc gagccacccg ctggtgcgct cattcgcggc tgaggatgag
     6841 gagtccggta gtgccgaaga acctttcgat gtgactgtgg gggcggaatc ccatctgcgc
     6901 atatcggttc acggctgcgg tgtcatcgtc cgcggcgtac gccagtacgt gggccgcagg
     6961 ccactggaaa tcctccgcaa gctgttcagg gacatcttcc gcgttggggt gcacgcatag
     7021 gtcgaacacg tacgtggttc ccagggaacc ccaccgccgt tccggcgcca gtgtagccag
     7081 accggccata tgaccatcgc gcgtttccag cacccgcgcc tggacgcgct tttcggggtt
     7141 tcgcaggtct gacaacacgt ggaggaatgt ggcttcggca tttgcgcacc cgaatttccc
     7201 gtacgtcacg ttgcggacgg cggggaggca ggtctgcagc ataaacacgt tggccgacgg
     7261 ccagtgcctc cacgcaggtt cggcgatttt gaggcgctcg agatcgtccc acaatgtgtc
     7321 cggactccat gaatcgggtg accaccacat tgacccgggg gcctcaggaa tcggcctgta
     7381 cccgtgcttc tggtaaatga agaacggggc cgactcgaac cccgtattga ggtacaacgc
     7441 gttccctttt cgctcccgga aatcggccat gtgaaagtcc atcagaacat cgcagattcc
     7501 cttgcgacga tgctctggcg gagtgtacac atgccccatg attccaagtc ccttgaactc
     7561 gacagtcatg atgttgccga cgagatccga gccaatcagc cccacgtaaa accgcgtttc
     7621 cagatccttg atccggccgg caaactcctc ttccaggtgg aactgccaga tatctccttt
     7681 gtgcgcgaga agggtccgga gcgctgggac aagatcagta ttccgacggt ctatcacacg
     7741 gcattcaaga ttttcgccgg ttttcagcac aaagtcatcg ttcattcgtt tctccggttc
     7801 gcgccgttca gcgtggtgat tgcctcttcg atcctctggg cgagaaaggg atggtgcgtg
     7861 tccatcctca cctcctgaag atcgctggac agactctgca tgcgcaacgc ggaaatgctg
     7921 ttggccgcct ctgcccgaac cgcccagtgt ccgtccgatc gaatgatcga acgtagcacc
     7981 ggagcgtacg acgtgtccga cgtgtcgacg cgaagcgcgc gcagggcatg catcgccgcg
     8041 aaggtacctt gcgatcccgc aatctgaagc aaaagcggcc gcgcagttgc ccttggcccg
     8101 agcgcctgct ccgcggcgta gcgcacgatc tggctctcat ccgacaggcg tcgtacaagc
     8161 gccgtgtccg atcgtgatgt cccgcatgtt ccaagagcta gagccgcggc gtgtcggacc
     8221 agcgcaactg aatcctccag aagagagacc agagcaggct cggaacgggt gtctttcagc
     8281 ctccacaggc tttcggctgc gacctgccgt gttttccaat cggggtgccg cagtagaccg
     8341 gtcagcggca gtaccgcatc tgaatacttc gaaaag
//
LOCUS       DAOTNG010000122         8344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7673_length_8344_cov_4.859935, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000122 DAOTNG010000000
VERSION     DAOTNG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7673_length_8344_cov_4.859935"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /gene="metX"
                     /locus_tag="P5179_07300"
     CDS             <1..437
                     /gene="metX"
                     /locus_tag="P5179_07300"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRS95058.1"
                     /translation="AAMDMKFGRRYRNKTKPDFDFGIEFEVESYLAYQGQSFVERFDA
                     NSYLYITRAMDYYDAAERWGGGNLVEACSRVTADTMVVSFSSDWLYPPAQCRELAAAF
                     CQCGKPVTYIEVPSSYGHDAFLVETEIVGKLLADFLARERKR"
     gene            434..1195
                     /gene="metW"
                     /locus_tag="P5179_07305"
     CDS             434..1195
                     /gene="metW"
                     /locus_tag="P5179_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRS95059.1"
                     /translation="MSRTPYEWEDRLVALLLRHAAEGEAIHEKLARLVDILEAEEKAL
                     GRVTGKPPEKEGEQRYDHRLIEQHIPDGASVLDLGCGAGTLLSRLIQEKRVRGQGVEI
                     DNAKVLECVANGVPVFQTNLDHGLGMFPDQSFDFVVLEKTVQTLNRPLPVLQEMVRVG
                     KQGIVTFPNFGHWQMRLDLALNGRMPVTRAFPYEWYESPNIRPLTLNDFDACVQCQGM
                     RIVEGHAVVGNTVRPLEPDDNLFAEEVMLIIEKAA"
     gene            complement(1284..2384)
                     /locus_tag="P5179_07310"
     CDS             complement(1284..2384)
                     /locus_tag="P5179_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95060.1"
                     /translation="MPFVRELGSERIEYRDRVTGVHITQITSFPVMSNHFYIEHPSFT
                     SDCSTLVIRSQRVPARAAPWDLYACDADGLNLRQLTDEDDANNFALAHEGSLAWYQRG
                     TSLWSVDVGTGERREVCGGPPGVLSMGYWCGCASPDGKFYFGYGRRRSDDQGVVIRYR
                     TDGTEAVVLAADPHLCHLHASPGGHGISFGGVNERGEGVQYGMNHDGTNLRVLPTATL
                     SHFTWAGTTKRFVGAGMWERRVIHTIEEGQRDSSVVVEGSFFWHTGISWDAEWTVADT
                     NWPNEGLMIVNIRARQYARLCLTDNTAGHPQWTHAHPAFSPDGKRVVCTSDRTGMCQV
                     YVADVPEEFKAWVATPNPEAAPGGRLPEWRPA"
     gene            complement(2435..2827)
                     /locus_tag="P5179_07315"
     CDS             complement(2435..2827)
                     /locus_tag="P5179_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRS95061.1"
                     /translation="MSSEAPSPRPRLLRWLLVTAGMLSVSLGFVGVFVPILPTTPFLL
                     LAAACFLRSSDRLYHWLMSHRVFGPYLQNYREHRALTLRAKVITTVGIWVSIGYAVVF
                     AAESVALRIALLLIGCAVNAHILRLKTV"
     gene            complement(2824..4008)
                     /locus_tag="P5179_07320"
     CDS             complement(2824..4008)
                     /locus_tag="P5179_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS95062.1"
                     /translation="MNSKTSGPSVLVTDFFPKGYATQGELSYQQEIQNAIEAAAERKQ
                     SVLFPPMVYKLDESGVRLHSHLTVWMYGAVFVLDARCARDGQAFFGDDLTDLRICGGE
                     IVGRNDVWPAGVNIRGLYLTGNTSNVRVQDMHMRDLSSNGIGVFGTPEMPARDVWVTD
                     CVIEHCCNRYGDYMSKDAGPEKGSVREDQGLIAFYHVEDFVVRGCRFERSRSDGTHFY
                     RCRRGHFVHNRVYGAQMGGYFLETCEEVLASDNVILDNGSRGVTIERGSRNCILNGNV
                     VSNSGREGLWAPDSAGLIVTSNIFDRNGRKPNDLERGRIWNANITIDDDPGEPTHSPT
                     HDYLVAHNIITTTESQIAAIRIDAEVARGIVVRDNTLLGDNRQILVQGRNEGHVVLEG
                     NV"
     gene            complement(4005..4928)
                     /locus_tag="P5179_07325"
     CDS             complement(4005..4928)
                     /locus_tag="P5179_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95063.1"
                     /translation="MNINEIRTACSQYRVVLEPDENTPEWWAGAPSVTRGDNGEFFLA
                     ARMREGKSPRGRRGYEVRILSSADGASFTPVNHIARDAAGVIGFERPALVRDPHSGAY
                     RLYGCCAFEDGWGIIRFQDAAHPKDIDPAKWQVVLKAENPADGFNHTVGYKDPFIFWD
                     RDRWRMFVIGYDRVERTYQFVSADGTKWEPASDRPALENTGWHSFFTRPACVLPLSVG
                     YLLVYEGSTILWRDPDYNIATGLAYSPDMETFIDLTPDEPLLKSTTAGPYHTWRYSHW
                     VHVGEEVYVYFEAARPNATNEIRLATFREGR"
     gene            5181..6821
                     /locus_tag="P5179_07330"
     CDS             5181..6821
                     /locus_tag="P5179_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRS95064.1"
                     /translation="MNRHILSVSVVSAFAVCVLVLSSCAHAPSVRTPHTSTTAQGTAT
                     RAGSFPINGIEAILDSTGLAEYAGEDDTVVESETDVEGIFRSAQERYEKAYECIASAR
                     AQIDTALGLLDEIRDTEDSTALTQRDEMILEFSHLMRQLGFVEQRGTVSMNGEIPLQM
                     NQFVEREIRSFQTVERSWFLGAYRRSGVYLPYIKARMREAGLPEQLAWLAFIESGYSP
                     RALSPARALGLWQFIPSTGYRYGMTRDRWIDHRMDFERSTTAAIAYLRDLHAMFGDWN
                     AALAAYNCGEGRVLRTINSQANQYMDDFWDLYVRLPQETARYVPRFHAVMAIMQDPLK
                     YGFSDLPQPEPPIAYDTLIVDRQVKLADIAERTGISADVLTRLNAELRTGITPQYPYR
                     LRVPVGTLDSVTVALNSAPTTSAPNLPDLITHRVKRGETIASIARKHHTTVTAIRQEN
                     GLRKNSRVRVGQILRIPVRSRASTPSQVASSSPRTARRNSNSHSSEGSPTTYVVRSGD
                     TLWSISQRYSLSVDELRNLNGLSRRSTIKPGQRLVVSR"
     gene            6903..7820
                     /locus_tag="P5179_07335"
     CDS             6903..7820
                     /locus_tag="P5179_07335"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS95065.1"
                     /translation="MSSQNWYRGNTHTHTNVSDGNASLEYTAEWYHKHGYDWLVITDH
                     NKFTPVEEIPYHPETPFLLIAGCEISMSAEGKPVHVCAVNPRENPNSVAMPTIIQTLQ
                     AGVDRTNTLGGIPIINHPNWCWAFTEYHMARVTDWALFEIFNPSTDCNNWGGGGELSM
                     EERWDRMLSAGMRVFAVAADDSHTFVGEFYGHLSLPGLGWVTVRAPELTTDAICAALK
                     AGDFYASTEIELEDYEVTPEWIRVRIKQFDGYKYTTKFIGMNGKVLEERNGTEAVYRR
                     RGDEPYVRARIWSSNGGHAWCQPVFPDSV"
     gene            7983..8312
                     /locus_tag="P5179_07340"
     CDS             7983..8312
                     /locus_tag="P5179_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRS95066.1"
                     /translation="MSASLRRLDREKRTIRAMIGMYCRAHHAPAGPLCPECAELLDYA
                     VARTDRCRFGADKPTCANCPVHCYKPAMRERIRTVMRYAGPRMLLRHPVLALLHLWDG
                     RRKAIER"
BASE COUNT         1688 a         2750 c         2342 g         1564 t
ORIGIN      
        1 acgcggcaat ggacatgaaa ttcgggcgcc gttatcgcaa caaaacaaaa cccgatttcg
       61 atttcgggat cgaattcgag gtcgagagtt accttgccta ccaggggcag tcgttcgtgg
      121 agcgctttga cgcgaacagc tacctctaca tcacgcgtgc catggactac tacgacgccg
      181 ccgaacggtg gggcggcgga aatctggtgg aggcctgttc ccgagtgacg gcagacacca
      241 tggtggtaag cttctcctcc gactggctgt acccacccgc ccagtgcagg gagctggcgg
      301 ccgcattctg ccagtgcggg aaacccgtta cgtacatcga ggtgccttct tcatacgggc
      361 acgacgcatt cctcgtggag acggaaatcg taggcaaact gctggccgat ttcctcgcca
      421 gggagcggaa acgatgagca gaacgccgta cgagtgggaa gaccgcctcg ttgcgctcct
      481 gttgcgacat gccgccgaag gcgaggcgat tcacgaaaaa ctcgctcgcc ttgttgacat
      541 actcgaagcg gaggaaaagg cccttgggcg cgtaaccggc aaaccgccgg aaaaagaagg
      601 cgaacagcgg tacgatcacc gcctcatcga gcaacacatc cccgatggcg cctcagtgct
      661 cgacctcggt tgcggggcgg gaactctcct ttcccgtctc attcaggaaa aacgagtgcg
      721 tggccagggc gtcgagatcg acaacgcgaa agtgttggaa tgcgttgcca acggtgttcc
      781 cgtcttccag accaacctcg atcacggcct cggaatgttc cccgaccaga gcttcgactt
      841 cgtggtgttg gaaaaaacgg tgcagacgct caaccgaccg ctacctgtgc tgcaggaaat
      901 ggtccgggtg ggaaaacagg ggatcgtcac attcccgaat ttcggccact ggcagatgcg
      961 gctcgacctt gccctcaacg gcaggatgcc ggttacccgc gccttcccct acgagtggta
     1021 tgaatcaccc aacatccggc cgctgacgct gaacgacttc gacgcctgcg tgcaatgtca
     1081 gggcatgcgg atcgttgagg gccatgcggt ggtggggaat acggtgcgcc ccctggaacc
     1141 ggatgacaac ctgtttgcgg aagaggtaat gctgatcatc gaaaaggccg cgtaaggtcg
     1201 tcaatcagcg gggcacggaa cgaaaagggc gcggtctgtg cgcccttttc tcatgtacgt
     1261 cgcccccgcc tcgagcgctc aaatcatgcc gggcgccact cgggcagccg tcctcccgga
     1321 gcggcttccg ggttgggcgt cgcaacccac gctttgaatt cctccggcac gtccgcgacg
     1381 tacacctgac acatccccgt ccggtcactg gtacagacca cacgtttccc gtcagggctg
     1441 aacgcggggt gcgcgtgcgt ccactgcgga tgccccgcgg tgttgtccgt caggcaaaga
     1501 cgggcatact gccgcgcacg gatattcacg atcatcaggc cctcattggg ccagttcgtg
     1561 tcggcaaccg tccactccgc gtcccacgaa atccccgtgt gccagaagaa cgaaccctcc
     1621 acaacaaccg aggaatcccg ctgcccctcc tcaatcgtgt ggatgaccct gcgctcccac
     1681 attcctgcac cgacgaaccg ctttgttgtg cctgcccagg tgaaatgtga gagcgtcgcg
     1741 gtgggcaata cacgcagatt cgtgccgtca tggttcattc cgtattgcac gccctcgccg
     1801 cgctcattca cgccgccaaa tgatatccca tgccctccgg ggctcgcgtg cagatgacac
     1861 aggtgagggt cggcggcaag aactactgct tccgtcccat cggtgcggta gcgaattacg
     1921 acgccctggt catccgatcg acgccttcca tacccgaagt agaacttgcc gtcggggctt
     1981 gcgcagccgc accagtaccc catggaaaga acgccaggtg gcccgccgca tacctctcgc
     2041 cgctccccgg tgcccacgtc aacgctccac aaactggttc cccgctggta ccacgccaga
     2101 gaaccctcat gcgcgagcgc gaaattgttt gcgtcgtctt catcggtaag ttgccgcagg
     2161 ttcaacccat cggcgtcaca tgcgtaaagg tcccacggag ccgcgcgcgc cggaacacgt
     2221 tgcgaccgaa tcacgagggt ggagcaatcg gaggtgaacg acggatgttc gatatagaag
     2281 tggttgctca ttaccggaaa gcttgtgatc tgtgtgatat gaacaccggt gacacgatcg
     2341 cggtactcaa tccgttcgct cccgagttcg cggacaaaag gcatctgtgt tcccccccgg
     2401 tctcgggtgc accttcaggc cgcgccggaa gccgtcacac cgttttcagg cgcagaatgt
     2461 gcgcgttcac tgcgcaccca atcaaaagca gtgcgatacg aagagcaacg ctttctgcgg
     2521 cgaatactac tgcatacccg atactcaccc agatcccaac ggtggtaatg actttcgcac
     2581 gcagcgtgag cgcgcggtgt tccctgtagt tctgcaggta cgggccgaac acgcggtgag
     2641 acatcaacca gtgataaagg cggtcggagc tccgaaggaa acacgccgcg gccagaagga
     2701 gaaagggcgt cgtgggaaga atcggcacga agacacccac gaatccgagc gatacggaca
     2761 gcattccggc agtcacaagc agccatcgca aaagccgggg ccgcggggat ggcgcctctg
     2821 aactcacaca ttcccctcca gaacaacgtg cccctcgtta cgtccctgaa cgagtatctg
     2881 ccggttgtct ccgagcaacg tgttgtcccg cacaacaatg ccgcgggcaa cttccgcatc
     2941 aatgcggata gccgcgatct ggctttccgt cgtggtgatg atgttgtgtg caacaaggta
     3001 atcgtgcgtg ggtgaatggg tcggttcccc cgggtcgtcg tctattgtga tgttcgcgtt
     3061 ccagatgcgg ccccgctcca gatcgttcgg cttgcggccg ttccgatcga atatgttgct
     3121 cgtgacaatc aaacccgcgc tgtccggagc ccagaggccc tctcgcccgc tgttggacac
     3181 cacattcccg ttcaatatgc aattccggga cccgcgctct atggtgactc cgcgggagcc
     3241 gttgtcgaga atcacgttgt ccgaagcaag cacctcctcg cacgtctcga ggaagtaccc
     3301 gcccatctgc gcgccataaa cccggttgtg cacgaaatga ccacgccggc aacggtagaa
     3361 gtgcgtcccg tcggaccggg agcgctcaaa cctgcaccct cgcaccacaa agtcctcgac
     3421 gtggtagaac gcgatcagcc cctgatcctc gcgcacactt cccttttcgg gaccggcatc
     3481 tttgctcatg tagtcgccgt acctgttgca gcagtgctcg atgacgcagt ccgtcaccca
     3541 gacatcacgg gccggcatct caggggtacc gaacacacca ataccgttgc tggataggtc
     3601 ccgcatgtgc atgtcctgaa ccctgacgtt gctggtgttc ccggtgagat acagcccgcg
     3661 aatgttcacc cctgcgggcc agacatcgtt ccggccgacg atttctcccc cgcagatgcg
     3721 gaggtccgtc aagtcgtcgc cgaagaacgc ctggccgtct ctcgcgcaac gcgcatccag
     3781 aacaaacact gcgccgtaca tccatacggt gaggtgcgag tgcagacgca cgcctgattc
     3841 gtcgagcttg tagaccatcg ggggaaacag gacagactgc ttccgttctg ctgccgcctc
     3901 gatcgcgttc tggatctcct gctggtaact cagttccccc tgtgttgcgt atcccttggg
     3961 gaaaaagtct gtgaccagca cgctcggccc cgaggttttg ctgttcatct tccttccctg
     4021 aaggttgcaa ggcgaatctc gttcgttgcg ttgggacgag ccgcctcaaa atagacgtac
     4081 acctcttccc cgacgtgcac ccagtgagaa taccgccagg tgtgatacgg cccggccgtc
     4141 gtgcttttca gcagcggctc atccggcgtg aggtcaatga aagtctccat atccggcgaa
     4201 tacgccaggc ccgtcgcgat attgtagtca gggtcccgcc acagaatagt cgacccttcg
     4261 tacaccagca ggtacccgac ggaaagcggc agaacacatg ccgggcgcgt gaaaaagctg
     4321 tgccacccgg tgttctcaag cgccggacgg tcgctggctg gctcccactt tgtcccgtcc
     4381 gcactgacaa actgataggt gcgctcaacc cggtcgtacc cgatcacaaa catccgccac
     4441 ctgtcgcgat cccagaagat gaacgggtcc ttgtagccca cggtatggtt gaacccgtcc
     4501 gcggggtttt ccgccttcag caccacctgc catttcgccg ggtcgatatc cttgggatgg
     4561 gccgcgtcct gaaaccggat gatgccccac ccgtcctcga acgcgcagca cccgtaaaga
     4621 cggtacgccc cgctgtgcgg gtcccgaaca agcgccgggc gctcgaaccc gatgacaccg
     4681 gctgcgtccc tggcgatatg gttcacgggc gtaaagctcg ccccgtccgc gctggaaaga
     4741 atccgtacct catagccccg ccgacctcga ggcgatttcc cctcgcgcat gcgtgcggcc
     4801 agaaagaact cgccgttgtc tcctcgcgtt acgctgggtg cgccggccca ccattccgga
     4861 gtgttctcgt caggctcaag caccacgcgg tactgcgaac acgctgtgcg gatctcattg
     4921 atattcatgg ttgttccctt ccatcaattc gttgtgcctg cctggcaatg actgaatagg
     4981 ccctattgta cctaatagcg cgcccgcaac ccgccaaccc ctatcccgcc gcaggccgaa
     5041 ttccggctaa cacttgccaa ccgcgcgcct cagttctatg tttcttgagt gccccatcgt
     5101 ctgcggctgt tgtgaccgcc ttcggtaaac cacttgtcca tctgatggtt acggccatca
     5161 tctcccgacg gaagcctgtg atgaacagac atatcctctc ggtgagtgtg gtgagtgcgt
     5221 tcgcggtttg tgttctggta ctatcttcct gtgcacatgc cccatccgta cgaactccgc
     5281 acacctccac aaccgctcag ggaactgcca cccgcgcagg atccttcccc atcaacggaa
     5341 tcgaagccat tctggattcg accggactgg cggaatacgc cggcgaggac gataccgtgg
     5401 tggaaagcga aaccgacgtc gagggaatct tccgctctgc ccaggaacgc tacgaaaaag
     5461 cgtatgagtg catcgccagc gcgcgggccc agatcgacac tgccctcggc ctgttggacg
     5521 aaatccgcga taccgaggac tccaccgcgc tcacccagcg cgacgagatg atcctcgagt
     5581 tctcccacct gatgcgccag ctgggctttg tggagcaacg cggaacggtc agcatgaacg
     5641 gggaaatccc gcttcagatg aaccagttcg tcgaacggga aatccgctcc ttccagacgg
     5701 ttgagcgatc atggttcctc ggcgcctacc ggcgcagcgg cgtgtacctc ccctacatca
     5761 aggcgcggat gcgcgaagcc ggactgcccg agcaactcgc gtggctcgcc tttatcgaaa
     5821 gcggatactc cccccgggcg ctttctcccg cccgcgcgct cgggctgtgg cagttcatcc
     5881 cctccaccgg ataccgctac ggcatgacgc gggatcgctg gatagaccac agaatggatt
     5941 ttgagcgctc caccaccgcg gccatcgcat acctgcgcga tctgcacgcc atgttcggcg
     6001 attggaatgc cgcgctggcc gcctacaact gcggagaggg acgcgtcctt cgaaccatca
     6061 acagccaggc aaaccagtac atggacgatt tctgggacct ctacgtccgc ctgccgcagg
     6121 aaaccgctcg ctatgtgccc cggttccacg cagtcatggc gatcatgcag gatcccctga
     6181 aatacgggtt ctccgacctg ccccagccgg agccgcccat cgcatacgat acgctcatcg
     6241 tcgaccgcca ggtgaaactt gcagatattg ccgaacgaac tggaatcagc gcggacgtat
     6301 taacccgctt gaacgccgag ttgcgcaccg gcatcacgcc tcaatacccc taccgcctgc
     6361 gcgttccggt aggcactctt gattcggtaa ccgttgccct gaattcggcg cccaccacca
     6421 gcgcgccaaa ccttccggac ctgataaccc accgggtgaa acgcggcgag acaatcgcct
     6481 cgattgcacg gaaacaccac accacagtca ccgccatacg gcaggaaaac ggcctcagga
     6541 agaactcccg cgtccgcgtc ggtcagatac tccgcattcc ggtccgttcg cgcgcgtcaa
     6601 ccccttcgca ggtcgcgtct tcgtcccccc gtacggcccg gaggaattca aattcacact
     6661 cgagtgaggg cagccccacc acctacgtcg tcaggtcggg cgacacactc tggtccatct
     6721 cgcaacgcta ctcgctctcc gtggatgaac tccgaaacct caacgggttg agccgacgct
     6781 ccacgatcaa gccggggcaa cggctggtcg tcagtcgctg atttcttcgc aatggctgca
     6841 accttcacac tttcgcactt gaggggcgca ccgcgcgcct ctctcctttt ccaggagccc
     6901 cgatgtcttc ccagaactgg taccgcggta acacccacac gcacacaaac gtctcagacg
     6961 gaaacgcgtc cctcgaatac acggccgagt ggtaccacaa acacgggtac gactggctcg
     7021 taatcaccga tcacaacaaa ttcacaccgg tcgaagagat cccgtaccac cccgagactc
     7081 cgtttcttct tatcgccggg tgcgaaatca gcatgagcgc cgagggaaaa cccgtgcacg
     7141 tgtgcgcggt caatccacgg gaaaacccga acagcgtcgc catgccgacc atcatccaga
     7201 cactgcaggc cggggtggac cgcacaaata ccctcggcgg aatccccatc atcaaccacc
     7261 cgaactggtg ctgggcattc accgaatacc acatggcacg cgtcaccgac tgggcgctgt
     7321 tcgagatatt caaccccagc accgactgca acaactgggg cggcggcggg gaactcagca
     7381 tggaagagcg ctgggaccgc atgctgtccg ccgggatgcg cgtctttgcg gtcgcggcag
     7441 atgattccca cacattcgtc ggggagttct atggccacct gtcccttccg gggctcggct
     7501 gggtgaccgt gcgcgcgccg gaactcacta ccgacgccat ctgcgccgcg ctcaaggcgg
     7561 gcgactttta cgcctcaacg gaaattgagc tggaagacta cgaagtcacg ccggagtgga
     7621 ttcgcgtgcg catcaagcag ttcgacggct acaaatacac cacgaagttc atcggcatga
     7681 acgggaaggt gctcgaggag cgcaacggta cggaagcggt ctaccggcgg cgcggcgacg
     7741 agccatacgt tcgcgcgcgg atatggtcat ccaacggcgg ccatgcctgg tgccagccgg
     7801 tgtttcccga cagcgtgtga ttcagaaccg gaggcgttgc ctgcgtccac cgcgcggaag
     7861 cctgtcgaag cgccctcgcg ttatccacgc ggcttgctcg gagaccgggg tgcggagtaa
     7921 tcctttcgtg tcgttcgtgt ggttcgtggt aacaaacttc ttaccggaat gacgcccgtt
     7981 ccatgtcggc ttcgctccgc cgtctcgacc gcgaaaaacg cacaatccgc gcgatgatcg
     8041 ggatgtactg ccgcgctcat cacgcacccg ccggtcccct ctgccctgaa tgcgcggagc
     8101 tgctcgatta cgccgtcgcg cgcaccgaca gatgccgctt cggcgcggac aaacccacct
     8161 gcgccaactg cccggttcat tgctacaaac ccgccatgcg ggagcgcatc cgtaccgtca
     8221 tgcgctacgc cgggccccgc atgctcctgc gccaccccgt ccttgcgctc ctgcacctgt
     8281 gggatggccg ccgcaaggcg attgagaggt aggggcggac ccccgtgtcc gcccgaaccg
     8341 gccc
//
LOCUS       DAOTNG010000123         8292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7724_length_8292_cov_5.924123, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000123 DAOTNG010000000
VERSION     DAOTNG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_8292_cov_5.924123"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="P5179_07345"
     CDS             <1..1074
                     /locus_tag="P5179_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS95067.1"
                     /translation="ISLEDAQNIASKAAKERRAFLVTDGMNHPLVPRQLAEQLGTDSF
                     VVAPLIFDDRVEGVIIADNAITRKSITELDLRSLELFAHQARLAVERSRLHAELERQL
                     EELREAHELLRANHEEMLRTEKLAAMGQVAAQVAHEIRNPLVAIGGFARSIRNSLRED
                     DQNRKFAEIIVEETARLENILREVLDFARPPHPRRDRVDVGSVLRRATEMLERELQKR
                     SIRINVQIPRDPVMVLGDAGQLRQVFHNLIQNAMDAMERVDTSSRLLSISLSCETDRQ
                     VAVTVEDTGGGFEADALEHVFEPFFTTKSHGTGLGLAICQQIVRDHGGTLSITNKPER
                     GATFTVRVPLLEKEVVNGENPGC"
     gene            1052..1405
                     /locus_tag="P5179_07350"
     CDS             1052..1405
                     /locus_tag="P5179_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS95068.1"
                     /translation="MAKILVVDDENALRVLYKHELTQDGHDVDLAGTADEAVAKVRET
                     AYDVVILDIRMPGKDGLEALTEMFAERRNIKVIINSAYSSYKENFISWAAEHYVVKSA
                     DTTELKEKIAQVLGG"
     gene            1402..2646
                     /gene="glgC"
                     /locus_tag="P5179_07355"
     CDS             1402..2646
                     /gene="glgC"
                     /locus_tag="P5179_07355"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRS95069.1"
                     /translation="MNTTKTRFTPGALLRNTLTVILAGGHGTRLYPLTKHRAKPAVPF
                     AGAYRIIDFTLSNCVNSGLRRVFVLTQYKSFSLDRHLQQGWSSLRYDMGEFILQLPPQ
                     ERMKRSWYEGTADAIFQNLYVFQEQRPEYTVILSGDHVYLMDYAPMLAAHENTGADLT
                     IAAIPVPRQDARRFGVLQVDASNRIVGFEEKPRDPASWRDDQTSLVSMGVYVFSTDVL
                     VRRVLEDAKNPQSEHDFGQNIIPGMVNRDAVFAYSMPAMSGCEPYWRDIGTIDSYWQT
                     SMDILRPDPPLNLYDPSWPVRTYRDHRPPPIVIDDGHPAGPFIRNSMVCAGARITSAR
                     IDRSIVALDNVVCPGADIEETVLMSRVRVGKGARIRRAIVDKDVDIPAGETIGYDLER
                     ERKRFLVTDNGIVVIPRGYVFD"
     gene            2680..3708
                     /gene="galT"
                     /locus_tag="P5179_07360"
     CDS             2680..3708
                     /gene="galT"
                     /locus_tag="P5179_07360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRS95070.1"
                     /translation="MQELRKDPVTDRWVIVSTERGARPTDFESLPRPKAPQLCPFCYG
                     FESTTPPEIYCVREAGTAPNTPGWSVRVVPNKFPALRIEGNLVREGVGMFDKMTGIGA
                     HEVIIEHPDHQRRFWDFSVQDIERVLGAYLQRTNDLKRDIRLRHMLVFKNEGEQAGAS
                     VAHSHSQLIATPVVPKRVREKLDGVNRYLSYKERCVYCDEIREEQRFGERVVFENDSF
                     ISFCPFASRFPFEVTLLPKTHRPDFSAIDGTERTALAEALRVTMRKLNEALNGPQYNW
                     IFLAAPTRLSGRESWEDFHWHVEIIPRLTKIAGFEWGTGFYINPTPPEHAAAYLRGVA
                     GCGSPGLD"
     gene            3745..5886
                     /locus_tag="P5179_07365"
     CDS             3745..5886
                     /locus_tag="P5179_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRS95071.1"
                     /translation="MPVLNVALLWHMHQPDYRDPETGMLALPWVRLHATKGYYDMVKV
                     IERADTHTHACFNFTPSLVSQLQGYVDGTLTDKYLELSRRPASDLSAEERRFVASNFF
                     HANRRTMIDCYPRYSELRTMSRLPSGSVRDFSDQDLTDLVVWFNLTWCGYGARADFPF
                     LMELIRQGRGFSEEQKLQMLALQRDIIAEILPSYRKAAEGKRIELTSTPFYHPILPLV
                     CTTDSAKAGLPDRELPAPPFVAPEDARHHVRRAIALHESVFGSRPDGMWPAEGSVSTE
                     AVQILAEEGVKWIASDQDVLFRSNPSLGQRDLYVPYSVGERGGQLSIVFRDKSLADKI
                     GFRYSGMTTDAAVNDFMDSLRAIWTGQPKSGGEQLVTIVLDGENAWEYYPDGGEAFLT
                     AMYSKVANSKDFRWTTISDYLARNPPRHAIRRIFPGSWINGNFDIWIGSSEENRAWEA
                     LRDTRSALISAQDRLSEKARQEAWEHIYIAEGSDWFWWYGDDFTTALQGEFDRLFRAH
                     LAAVFELINVPVPAWLVKPIRKGRELAASKPANLISPTLDGRSTSYYEWAGAGHFDAR
                     SADGTMAREAPLVSAIAFGADHHRWYLRIDWSRPLAPNERSELEVVCVFPNRPGTQVI
                     IGPFGFETRDIPVKIVEHGTEVQITPQAVFRDVVECAVPFLLLGAAPGTLVEFVVSVR
                     QGGDEIERWPRDGVLAFDVPTDTFELEHWTV"
     gene            5888..6856
                     /locus_tag="P5179_07370"
     CDS             5888..6856
                     /locus_tag="P5179_07370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRS95072.1"
                     /translation="MVLDPRTLRVKSIAYIREALANCGAVSDSVIRAMEEDDRAAVRR
                     MAEQLKKAPRRPHTDMLVHEQQCWQSGSVRVAGVDEAGRGPLAGPVYAAAVILPRDGI
                     PSGINDSKRLSARQREQLDPLIRACALAVHVASVGVEEIDRVNILQASLKAMRTAIAG
                     LGHPPPDHVLVDGNITPGSGLPETAIVDGDSKSVSIAAASILAKVARDRFMEEMDTRY
                     PGYGFARNKGYGTREHLEALARLGPCPIHRRSFQHVACASEYPTHSDLIAFFKRSLVV
                     SESLEELRSVAAIIAEKKNVLPPEEVPALRRLYRQQFKLLRSRCDT"
     gene            6853..7224
                     /locus_tag="P5179_07375"
     CDS             6853..7224
                     /locus_tag="P5179_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRS95073.1"
                     /translation="MKRGRLGEELAAGYLKRAGWTLCARNWHAGRAGEIDIVGWDGDI
                     LVFVEVKTANTSRYGHPVTWVDARKQSQLARLAELFVAKGDFVFSAMRFDVITIDAEK
                     QPPAITHLVDAFRPDIVGETG"
     gene            complement(7261..8250)
                     /gene="dctP"
                     /locus_tag="P5179_07380"
     CDS             complement(7261..8250)
                     /gene="dctP"
                     /locus_tag="P5179_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRS95074.1"
                     /translation="MVTSLSTKLRVALLTAVLSGGLLSACAPRQTEVLLRVRHGAPPA
                     MPMHRALLKVASILEERSRGRIHLVVEAGLSLPYQIDEMLKGSDEISMSGMGSLSDYY
                     PPLGALEAPYMFRDIDHFYHTVETPFWRSLVTEVERRSGLHVIDMWHQGVRQVTLRDQ
                     AARSPAEFGTIKLRVPPSQMYVECARVLGALPTTMNINDVRTAIRAGTVDGQENPLPT
                     IEAMHFHDVCSYLVLTNHSITPIMPIVSAVFWRTLSERDRLLITEAFHEGGRYNRQIV
                     EEEERELIHEFEQSGMIVIRPDLRLFRDRAEKVWRRYREVWGDSLVARIQAVR"
BASE COUNT         1775 a         2311 c         2488 g         1718 t
ORIGIN      
        1 atctccctgg aggacgcaca gaacatcgcc tccaaggcag ccaaagagcg gcgcgcattc
       61 cttgttacgg acggtatgaa ccacccgctg gttccccgcc agctcgccga acaactgggc
      121 accgattcct tcgttgtcgc cccgttgata tttgacgacc gggtcgaagg ggtgatcatc
      181 gcggacaacg ccatcacgcg caaatccatt accgaattgg atttgaggag cctggaattg
      241 ttcgcgcatc aggcacgtct tgcggtggag cgatcgcggt tgcacgcgga actcgagcgc
      301 cagctcgaag aattacggga agcacacgaa cttctcaggg cgaaccatga agaaatgttg
      361 cgaacggaaa aactggccgc catgggacag gtagcagcgc aggtggcaca tgaaatccgc
      421 aaccccctcg tcgccatcgg aggatttgcg cgcagcattc gcaactcgct tcgtgaagac
      481 gatcagaaca ggaagtttgc ggagatcata gtagaggaaa cggcccgtct ggagaacatc
      541 ctgcgggagg tgctggattt tgcccgtccc ccgcacccac gtcgtgaccg cgttgatgtc
      601 ggctcggttc tgcggcgtgc cacagagatg ctggaacggg aacttcagaa acgtagtatc
      661 agaattaacg tgcagattcc gcgcgacccg gttatggtcc tgggtgacgc gggccagttg
      721 cggcaggtgt ttcacaatct tattcagaac gccatggacg cgatggaacg cgtcgacacg
      781 tcctctcgat tgctgtccat atcactttcc tgtgaaaccg atcgtcaggt tgccgtgacg
      841 gtcgaggata caggaggcgg gtttgaagcg gacgcgctcg agcatgtctt cgagcctttc
      901 ttcaccacaa agtcgcatgg tacggggttg ggtcttgcga tctgccagca gatagtccgg
      961 gaccacggag gtactctttc tattaccaac aaaccggagc gtggcgctac gtttacggtt
     1021 cgggtgccac tgctggaaaa ggaggttgtg aatggcgaaa atcctggttg ttgacgacga
     1081 aaacgccctt cgcgtgctgt acaaacatga actgacacag gatggacatg acgttgacct
     1141 ggctggcact gctgacgaag cggtcgcgaa ggttcgagag acagcgtacg acgttgtgat
     1201 tcttgatatc cggatgccgg gaaaagacgg tctggaggca cttactgaga tgttcgccga
     1261 gcggcggaac atcaaggtga tcatcaactc ggcgtattcc agctacaaag agaacttcat
     1321 cagctgggct gcggagcact acgtcgtgaa gtcggccgac accactgagc taaaagaaaa
     1381 aatcgcgcag gtgctgggag gatgaatacc accaaaactc ggttcacacc gggcgctctc
     1441 ctgcggaaca cactgaccgt aatccttgca ggcgggcatg gaacccgttt gtatccgctc
     1501 acgaagcatc gcgcaaaacc cgcagttccc tttgcggggg cctatcgcat tatcgacttc
     1561 accctttcca actgcgtgaa ttcgggtttg cgcagagttt tcgttttgac ccagtataag
     1621 tccttcagtc tggatcggca cctccaacag ggatggtcca gcctccggta tgatatgggt
     1681 gaattcatcc tgcaattgcc accgcaggaa cgcatgaagc gttcgtggta tgaaggtacc
     1741 gcagacgcga tctttcagaa cttgtatgta tttcaggagc aacggcccga gtacacggta
     1801 attctctcgg gcgaccacgt gtatctcatg gattatgctc cgatgctggc ggcgcacgag
     1861 aatacggggg cggatctcac gattgccgcc atcccggtgc cccgtcagga cgcgaggcgt
     1921 ttcggtgtac tccaggtgga tgcgtccaac cgaatcgtgg gatttgagga gaaaccgcga
     1981 gacccggcat cgtggagaga cgatcagacc agcctcgtat ccatgggcgt ctacgtcttt
     2041 tccaccgacg ttctggttcg gagagtactg gaagatgcga aaaaccccca aagtgagcat
     2101 gatttcggcc agaacatcat cccgggcatg gtcaacagag acgcggtctt tgcctactcg
     2161 atgcccgcga tgagcggatg cgagccctac tggagggaca tcggcacgat cgacagttac
     2221 tggcagactt caatggacat cctgcggccg gaccctcctc tcaacctgta tgacccctcg
     2281 tggccggtgc gaacgtaccg cgatcatcga ccacctccga ttgtcataga cgatggccac
     2341 cctgcggggc ctttcatcag gaacagcatg gtgtgcgcgg gggcccgtat cacgtccgcg
     2401 cgtatcgatc gttcgatcgt ggcactcgac aatgtagtct gccccggagc ggatatcgag
     2461 gaaacggttc tgatgagccg ggtgcgcgtg ggcaaaggtg cgcgaatccg aagggccatc
     2521 gttgacaaag atgtggacat cccggcgggg gagaccatcg gatacgatct tgaacgagag
     2581 cggaaacgtt tcctcgttac cgacaacggc atcgtcgtca tcccgcgtgg ctacgtcttt
     2641 gactgattcc ctgtttgcac gaataccgga ggcagtttga tgcaggaact ccgcaaagac
     2701 ccggtgacag accgctgggt catagtttcc accgagaggg gagcgcgacc aacggatttc
     2761 gaatcgttgc cgcggcccaa ggctccgcag ctttgtccgt tctgttacgg attcgaatcc
     2821 acgacgccgc cggagattta ctgcgtgagg gaagcgggta ccgcacccaa cacgcccggc
     2881 tggtccgtgc gggtggtgcc caacaagttc cctgctctgc gaatcgaagg aaaccttgtc
     2941 cgggaagggg tcggcatgtt cgacaaaatg accggaatcg gtgcgcacga ggttatcatc
     3001 gagcatcctg atcaccagcg gcgtttctgg gacttttcgg ttcaggatat tgagcgcgtc
     3061 cttggcgcat atcttcagcg aacaaatgac ctgaagcgcg acattcgatt gcggcatatg
     3121 ctggtgttca agaacgaagg cgagcaggcg ggtgcttcag tcgctcattc gcattctcag
     3181 ctcattgcca cgcccgtcgt tcccaagcgc gtgcgtgaga aactggacgg cgtcaaccgg
     3241 tacctgagtt acaaagaacg gtgcgtgtac tgcgatgaga ttcgtgagga acagcggttt
     3301 ggagagcgcg ttgtttttga aaatgatagc ttcatttcgt tctgcccttt cgcctcccgc
     3361 tttccgttcg aagtgacgct cctgccgaaa acgcatcgcc cggatttttc cgccatcgac
     3421 ggcacagaac gaacggcact ggcagaggcg ctgcgggtca cgatgcggaa actcaatgaa
     3481 gcgctgaacg ggccccagta taactggatc tttcttgcgg cgccaacgag attatcggga
     3541 cgcgaatcgt gggaggattt ccactggcac gttgagataa tcccgcgcct taccaaaatc
     3601 gccgggttcg agtggggaac cggcttctac atcaatccaa ccccgcccga acacgcggct
     3661 gcgtaccttc gtggcgtggc cgggtgcgga tcgccagggt tggattgatg ttcacttccc
     3721 ctccttccgg agcctttccc acagatgccc gtacttaacg tcgcactcct ctggcatatg
     3781 caccagccag attaccgtga tcccgagact ggcatgctgg cgctgccgtg ggtgcgcctt
     3841 cacgccacca aagggtatta cgacatggtg aaggtgatcg aacgggcaga tacacatact
     3901 cacgcctgtt tcaatttcac gccgagcctc gtttcgcagc ttcaggggta cgttgacgga
     3961 acgcttacgg acaagtacct cgaactttcc cggcggcctg catcggatct ttcggcagag
     4021 gaaagacgct tcgtcgcgag caactttttc cacgcgaacc gaagaacgat gatcgactgt
     4081 tacccgcggt attccgaact gagaaccatg tcgcgtctcc cttccgggag cgttcgagat
     4141 ttctcggatc aggacctcac tgatctcgtc gtgtggttca atctcacctg gtgtggctac
     4201 ggcgcgcgcg ccgatttccc cttcctgatg gaattgatcc gacaggggag agggttttcc
     4261 gaggagcaga agctccagat gctcgctctt cagcgggaca tcatcgcgga gattctcccg
     4321 tcctaccgca aggcagcgga agggaaacgg atcgaattga cttcgactcc gttttaccat
     4381 ccgatcctgc cgttagtgtg cacaacggat tcggcgaaag cggggcttcc ggacagggaa
     4441 ctcccggccc cgccgttcgt cgcgccggag gacgcaagac accacgtaag acgcgccata
     4501 gcactgcacg aatccgtttt tggaagccgc cccgacggta tgtggcctgc agaaggatcc
     4561 gtgagtaccg aagcagtaca gattctcgcg gaagagggtg tcaaatggat cgcatcggat
     4621 caggacgtgc tgttccgttc gaatccgtcg ctgggacaac gagatctgta tgtgccgtat
     4681 tccgtcggcg aacgaggtgg tcaactctcg atcgtgttcc gggacaagtc tctcgctgac
     4741 aaaatagggt tccgctattc cggaatgacg actgatgcgg cagtgaacga cttcatggac
     4801 agtctgcgag cgatctggac cgggcaaccc aaatccggtg gcgaacagct cgttactatc
     4861 gtgctggacg gcgaaaacgc gtgggagtac tacccggacg gtggggaagc attcctgacg
     4921 gccatgtact caaaggttgc aaattcgaag gatttccgat ggactaccat ctccgactac
     4981 cttgccagaa accctccgcg acatgccatc cgaaggattt tcccagggtc gtggatcaat
     5041 gggaattttg acatctggat aggatcgagt gaggaaaatc gtgcgtggga agcactgcgt
     5101 gacacacgca gtgctctgat ttcggcacag gatcggttat ctgagaaggc tcggcaggag
     5161 gcctgggagc acatctacat cgcggaaggg tccgactggt tctggtggta cggggatgat
     5221 ttcaccaccg ccttgcaggg cgagtttgac cggcttttcc gcgcgcatct ggcggctgtg
     5281 ttcgaactga tcaacgtacc ggtgcccgcg tggttggtga aaccgatcag gaaaggccgg
     5341 gaactggccg ccagcaagcc ggcaaatctc atttctccga ccctcgacgg ccggtcaacg
     5401 agttactacg aatgggctgg cgccggccat ttcgatgctc gttctgcaga cggcaccatg
     5461 gcaagggaag cgccgctggt ctcagcgatc gcgttcggcg ccgatcatca caggtggtac
     5521 ctcagaattg actggtccag accgttggcg ccgaacgagc gttcggaatt ggaggtcgtt
     5581 tgcgtctttc caaacagacc tggtacgcag gtcatcatcg gcccgtttgg gttcgagacg
     5641 cgggacatcc ccgtcaagat tgtggaacac gggactgaag tccagatcac tccgcaggcc
     5701 gtttttcgcg atgtggttga gtgcgccgtg cctttccttc tactcggcgc cgccccgggg
     5761 acgctcgtgg aatttgtggt gagtgtccgc caagggggcg atgaaatcga gcgttggccg
     5821 cgtgacgggg tactggcgtt cgatgtgccc accgacacat ttgagctgga gcactggacc
     5881 gtgtaacatg gtgctggatc ccagaaccct ccgggtgaaa tcgatcgcct acatacgaga
     5941 ggcgctggcc aattgcgggg cagtctctga ttctgtcatc cgtgcgatgg aggaggatga
     6001 ccgggcagcc gtccgcagga tggcggagca actgaagaag gcgcctcgcc gcccgcatac
     6061 cgacatgtta gtccacgaac aacaatgctg gcaaagcggt tccgtgcggg tcgccggcgt
     6121 ggacgaagca ggcagggggc ctctcgcggg tcctgtgtac gccgccgcgg taatcttacc
     6181 gcgcgatggt atcccatctg gaatcaacga ttcgaaacgg ctgtccgccc gacaacggga
     6241 gcaactggat ccgcttatcc gagcgtgtgc gctggcagtg cacgttgcat cggttggtgt
     6301 cgaggaaatt gaccgtgtga acatacttca ggccagcctg aaagcgatgc gcacggcgat
     6361 cgcaggcctt ggacaccctc ctcccgatca tgtgctggtg gatggcaaca tcacccccgg
     6421 aagcgggctc ccggagactg caatcgtcga tggcgactcc aaatccgttt ccattgcggc
     6481 cgcgtcgatc ctggcgaagg tcgcgcgtga tcgcttcatg gaggaaatgg acacgcggta
     6541 tccgggttac gggtttgcgc ggaacaaagg gtacgggacg cgggaacatc tcgaggcgct
     6601 tgcacgtctc ggcccgtgtc cgattcacag gcggtctttc cagcatgttg cctgcgcgtc
     6661 tgaatatccc acccattcgg atcttatagc gttcttcaag cgttcgcttg tcgtgtcaga
     6721 gtccctcgaa gagctccgtt ctgtcgccgc aatcatagcc gaaaaaaaga acgtgctgcc
     6781 gcccgaagag gtaccggcgt tgaggcggtt gtaccggcaa caattcaagc tacttcgcag
     6841 caggtgcgat acatgaaacg cggcagattg ggggaggaac tggcggcggg gtacctgaag
     6901 cgggccggtt ggacgctctg cgcccggaac tggcacgcag gcagagccgg tgagatagac
     6961 attgtcgggt gggacggcga tattcttgtt ttcgtggagg tcaaaacggc gaatacaagt
     7021 cgctacggac atccggtcac gtgggtggat gctcgcaaac aatctcaact tgcgaggctg
     7081 gctgagttgt ttgtcgcaaa gggagatttc gttttttcgg cgatgcgttt cgacgtcata
     7141 accattgacg cagagaagca gccgccggcg atcacccatc ttgtggacgc ctttcggcct
     7201 gacatcgtgg gagagacagg gtgatggtgt gcgcgcggcg agagagaaag agaaaactgg
     7261 ttatctgaca gcctgaattc tggcgaccaa cgaatcgccc cagacttcgc ggtaccgcct
     7321 ccacaccttt tccgctcgat ctctgaacag gcggaggtcc gggcggatca caatcatgcc
     7381 ggattgctcg aattcgtgaa tcaattcccg ttcttcttcc tccacgatct gccggttgta
     7441 tctcccacct tcatggaagg cttccgtaat gaggagccga tcacgttccg acagagtccg
     7501 ccagaacacg gcgctgacga taggcatgat cggcgtgatg ctgtgattgg tgagaacaag
     7561 atatgagcac acgtcgtgga aatgcatcgc ttcaatcgtt ggcaacgggt tctcctgacc
     7621 gtccaccgtg cccgctcgaa tcgccgtgcg aacgtcattg atgttcatcg tcgtgggaag
     7681 cgctccgagc acacgtgcgc attcgacgta catctgagaa ggcggtacgc gcagtttgat
     7741 cgtcccgaac tccgcagggg aacgggccgc ctgatcccgc agagtgacct ggcgtacgcc
     7801 ctggtgccac atgtcaatca cgtggagtcc agatcgccgt tcaacctcgg tgacgaggga
     7861 gcgccaaaaa ggtgtctcga cagtgtgata gaaatggtcg atatcgcgaa acatgtacgg
     7921 cgcttcaagg gcgcccaggg gtgggtagta atcggagagg cttcccatcc ctgacatcga
     7981 gatttcgtcg ctccctttga gcatctcatc tatctggtac ggaagcgaaa gtcccgcttc
     8041 caccaccagg tgaatccgac cgcgagaccg ttcctccagt atggacgcaa ctttgaggag
     8101 tgcgcggtgc atcggcatgg cggggggtgc accgtggcga acccgtaaca ggacctcggt
     8161 ttgtctcggt gcgcacgctg acaacagccc gcccgaaagg accgcggtca gcaacgccac
     8221 acggagtttt gtggaaagag aagtcaccat cggcgccgcg cattccccgc atcaaaaacc
     8281 cgaaagggcc tc
//
LOCUS       DAOTNG010000124         8227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7792_length_8227_cov_4.732379, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000124 DAOTNG010000000
VERSION     DAOTNG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_8227_cov_4.732379"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2865)
                     /locus_tag="P5179_07385"
     CDS             complement(<1..2865)
                     /locus_tag="P5179_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95075.1"
                     /translation="MRPPEDVYQGELVRYPGPWGFNIPRSHIILVSDEQLEQLQDPDA
                     LVDLSLSPTKDLRSLRQVCEQAAAWGVRTLMFAFDHFWNQYREGQGNKPRELLPDTDE
                     YISRIAKVSHFVQQYGIGLELSLLSPLELGKGFRNATGETGKWMHVREGIRDPSTGAF
                     FVQLWRQKRWGNNKGAFDVELENVRAFAFRERQIGGTHLFSVVRDEIREITDCVEMDP
                     GCTGAYSAGDYLAERVAVRGNGMADVGDLDRVAVVLTYKTPEMDYFSPSATAFLQDLL
                     ERYRKAGVKLNGLYADEMHIQQDWGYFSHHDHGQLALRYVSPHLERRFSELFGREYAD
                     FAPWMVYFCSGQHDFLPTTGAASFCQHVLGSTQDEISATFLLRQRYYHVLEGTVVDLM
                     ADAKHYAERLAGHTLPARAHVTWAESPTIDDSEPSPLPHYTQLYEYTQSFRWSNTVHQ
                     AASACQDYFRWNDFLTGGGNDHAEGGYADRNYFALALACSTGNLNEVPYAYAAHWGMP
                     KEIAQRRSALVDVFGACASPTFQAVEDSQHRTSNVLMLYPIDLVSVDERFGSWMVQYG
                     YADYITAEKLCELGRVTAAGEIAVKDRRYRTLVALYEPFPSEQLMFLMERLVNQGGRV
                     VWSGTPPFLHAEGTPVRHAWERLFGVTCGHEDAPTGVPVPGRTIRFCGPLDGVPAQII
                     LTNTVVDHIYPFVVSGGSEVSAKVGKWVVGAVRSAGMRGGKAVALGFRPRDDQSQSLG
                     DEVRTWFEILVKLGSYPPSGLFGDVNDNPEYLSRTKPWLAQAFPNGAIAVAPHFRTYE
                     EGWHGGFHRDDEADRKWLNDHPLPSNEICLEQELIHGVRLTYRGEGAVALRLDGDGDL
                     IAFAGSACSGVTVNDRSFVFSDHPVRHLGWSPVPPEQRVPGGAVFRVWIDSDHDISVP
                     VPAGLPPLRWWCQGPKPGCRGVPVTASKTDGVVHFGVPQGI"
     gene            complement(2890..3645)
                     /locus_tag="P5179_07390"
     CDS             complement(2890..3645)
                     /locus_tag="P5179_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS95076.1"
                     /translation="MGNKPVWGRFEELRPDQLEQIVERWPVAYWPLGLIEHHGWALPV
                     GFDGVKAVRFCERIAAATGGVVLPCLWWGSHGGHGDFKWTFYQDPVVGERVLDVTVRK
                     LIDFGFRAIVLHAGHYPWQGVLDKVLSPVRDEHPEVLFIWGTECTIGGEGLRDVPGDH
                     AARWETSYGLALLPDLVDTGALREGRGPEAWPNATPPPEERRYRGVVFDTQHPLFAQL
                     GEDARKASAEEAEFYLSRIAEAITQRVRNHLER"
     gene            complement(3867..5558)
                     /locus_tag="P5179_07395"
     CDS             complement(3867..5558)
                     /locus_tag="P5179_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95077.1"
                     /translation="MRVEAFFAACALLAMSSCASLEVPDAPPHETTVQQQSPRPEAIL
                     RLLAAKRAERRGDSAGALAQWQAAVRADSSSPSLRLGLAKAYSVQRNDSLALLNGRRS
                     VLLDSNYVDGHLFLAGLYEGRNDFASAATHLEPAFRESKSPETGWQLARLYARLAKPE
                     ESRRVYQTMADSPTAHPDDVLAWAEQVQMSPVKGASDIFYTTCMRRWPRNEEGIGAYA
                     EYLARSGRRVEAERLLREWSASHPDSREVPKRYIQLLVAQDRWADADSVWMRIRRESP
                     DDLVEQKGWITYLTKRGQIPLAIANAQRLIETHPSDGDGYVLLGQAHAAQGDLHAAAA
                     AFEQAASQDSSLEALSELAYAQAGLKQYAAAERAARQGLSKYPGDERLLSLYSAALSS
                     QNKWREAVPVLRALAERDTTDTDRLFDLGAGLERAGEFDSAVVVFKRLLAINRHHADA
                     LNYLGFMFADRNVHLAEAESLLTEAVRLEPRNAAFLDSMGWLFFRQGRYQEAKQHLAD
                     AIEIDDSVAEMHAHLGDVLSALGDRDNAREAYRAALRLDPANRTVERKLRLLERR"
     gene            complement(5698..5961)
                     /gene="rpmA"
                     /locus_tag="P5179_07400"
     CDS             complement(5698..5961)
                     /gene="rpmA"
                     /locus_tag="P5179_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018179605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRS95078.1"
                     /translation="MAHKKGVGSSRNGRDSNAQRLGLKRFGGELVNAGTIIVRQRGTR
                     FHPGLHVGRGGDDTLFALATGHVKFAPHKNGKGKVVSIEPLET"
     gene            complement(5986..6300)
                     /gene="rplU"
                     /locus_tag="P5179_07405"
     CDS             complement(5986..6300)
                     /gene="rplU"
                     /locus_tag="P5179_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRS95079.1"
                     /translation="MQAVVRVAGVQARVAPGDKIFLPRIPGEIGTVTTLGEVLLLSDG
                     DKITVGKPVVEGASVQAEVLDHARDAKVLVFKKKRRKRYRRLRGHRQSYTQVLIKEIV
                     AA"
     gene            complement(6392..7945)
                     /locus_tag="P5179_07410"
     CDS             complement(6392..7945)
                     /locus_tag="P5179_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRS95080.1"
                     /translation="MKTEIIVNSATQETRIAILEDGRLVEILVERPDTQRLVGNIYKG
                     IVTSILPGMQAAFVDIGMEKSAFLHARDVGGSPERLDDEEDEDEEDDDEEAPRSGRET
                     KKYTPIQELLTKGQEVIVQITKEPIGTKGPRVTTDVSLPGRFSVLMPYSNHVGVSRKI
                     LSWSEKRRLKALAARLRPENCGIIVRTVAEGQGEDDLTRDIDDLKRTWERVHRRAKRM
                     KAPALIHSELGMTTSLMRDLLSEDVERVVVDSKSVYNDIIKYLKGTAPQLRDRVLLYD
                     QKEPVFDAYGIEQEIANATNRRVPLRKGGFLVIDHTEAMVTIDVNTGRYVGRNNQEQT
                     IVNINLEAGQEIARQLRLRDIGGIIAIDFIDMAIEANRKKVWEEFNKALRHDRSRINV
                     AERLSEFGVLEMTRQRVRPSLLYTFSEPCPLCKGSGRVQSPDTTVTKLERWLKRLRAA
                     GGEKKLVLEVHPTVGSYLRENDQRILKALRRTTGAQIILEDNPSLEIDAYRFSSLKTA
                     EDLTGKYEA"
BASE COUNT         1643 a         2577 c         2271 g         1736 t
ORIGIN      
        1 gattccctga ggcaccccaa aatggaccac gccatctgtc tttgacgcag tcaccggaac
       61 acccctgcag ccaggcttcg gcccctgaca ccaccacctc aacggcggaa gtcctgccgg
      121 aactggtacg gagatatcgt ggtcagaatc aatccacacc cggaaaaccg ctcctccggg
      181 tacccgctgt tcgggcggaa caggcgacca gcccagatgc cgaacagggt gatcggagaa
      241 aacaaaggaa cgatcgttga cggttacccc ggagcatgcg gaaccggcaa aagcaatgag
      301 atcaccgtcg ccgtcgagcc ggagggctac cgcgccttcg cctcggtacg tcagccgcac
      361 gccgtgtatc agttcctgtt ccagacagat ctcgttcgat ggcaacggat gatcgttcag
      421 ccatttcctg tcggcctcat catcacggtg aaacccaccg tgccatccct cttcgtaagt
      481 ccggaaatgg ggtgccaccg cgatcgctcc gttgggaaac gcctgcgcca accacggctt
      541 cgttcgagaa aggtactcgg gattatcgtt gacgtctccg aacaacccgc tcgggggata
      601 ggacccaagc ttcacaagga tctcgaacca cgtccgaacc tcatccccaa ggctctgtga
      661 ttgatcgtcc cgcggacgga accccagagc caccgctttg cccccgcgca ttcctgcaga
      721 acgaacggcc cctaccaccc atttcccgac tttggcagac acctccgatc ctccgctgac
      781 aacaaacgga tagatgtgat caaccacggt gttggtgagg atgatctgcg cgggaacgcc
      841 gtcaagcgga ccgcagaacc gtatggttct gccgggaacc ggcacacccg tcggagcgtc
      901 ttcgtgccca cacgtaactc caaacagacg ttcccatgca tgccggaccg gagttccctc
      961 cgcatggagg aacggcggcg tgccggacca gactacacgc ccaccttggt tgacaaggcg
     1021 ctccataagg aacatcaact gctcggaagg aaatggctcg tagagagcca cgagcgtcct
     1081 gtagcgccga tctttgacgg caatctcccc tgcagccgtc actcgtccca gttcgcagag
     1141 cttttccgca gtgatataat ccgcgtaccc atattgcacc atccagcttc cgaaccgttc
     1201 atccaccgac acgagatcta tggggtagag catcaggacg ttgcttgtcc ggtgctgaga
     1261 gtcttcaact gcctgaaagg tgggcgatgc gcacgcaccg aacacgtcta caagcgcgga
     1321 tcggcgctgg gcgatttctt ttggcatacc ccagtgcgcc gcgtaggcat atggcacttc
     1381 attcaggttt ccagtcgagc acgcaagtgc aagcgcaaag tagttgcggt ctgcgtatcc
     1441 accttcggcg tgatcgttgc ctccaccggt aaggaagtca ttccatcgga aatagtcctg
     1501 gcatgccgac gcagcttgat gaacagtgtt cgaccaccgg aacgactggg tgtattcata
     1561 caactgcgtg tagtgcggca gcgggctcgg ttccgagtcg tcaatcgtgg ggctctccgc
     1621 ccaggttaca tgcgcgcgtg ccgggagcgt atggcccgcc agtcgttccg catagtgctt
     1681 cgcgtcagcc atgagatcta caaccgttcc ctccagcacg tgatagtaac gctgccggag
     1741 cagaaaggtg gcggaaatct cgtcctgcgt actcccgaga acgtgctgac agaatgatgc
     1801 cgcaccggtg gtgggaagga aatcgtgctg accggaacag aagtacacca tccacggggc
     1861 gaaatcggcg tactcgcggc cgaaaagctc ggagaaacga cgctccaggt gcgggctcac
     1921 gtatcgcaga gccaactgac cgtggtcgtg gtggctgaag tatccccagt cctgttgaat
     1981 gtgcatttcg tccgcgtaga gaccgttcag tttgacgccc gccttccggt accgttcgag
     2041 gagatcttgc agaaacgccg tagcggacgg gctgaagtaa tccatctcgg gggttttgta
     2101 ggtgagaact accgccacgc ggtcgagatc tcccacatcc gccatcccgt ttccgcgtac
     2161 ggccacacgt tctgcaaggt aatcacccgc tgagtacgca ccggtgcagc cgggatccat
     2221 ctccacacaa tcagttattt ctcgaatttc gtcacgaaca acggagaaca gatgcgtgcc
     2281 cccgatctgt ctctcccgaa acgcaaaggc gcggacgttt tccaactcca cgtcgaacgc
     2341 gcccttgttg ttaccccacc gtttctgacg ccacagttga acgaaaaagg cacccgtgga
     2401 aggatcgcga atcccctcgc ggacatgcat ccacttcccg gtttcgccgg ttgcattccg
     2461 aaaccctttt cccagttcca agggactcag caggctcagt tcgaggccga ttccatactg
     2521 ctggacgaaa tggctcactt tcgcgatccg ggagatgtac tcgtctgtgt ccgggagcag
     2581 ttcgcgcggt ttgttcccct gaccttcccg gtactggttc cagaaatgat cgaacgcgaa
     2641 catgagcgtt cgcactcccc acgccgcggc ttgctcacac acctgacgaa gcgagcgcag
     2701 atccttcgtc ggactcaggc tcagatctac cagcgcatca ggatcctgaa gttgctccaa
     2761 ctgctcatca cttaccagaa ttatgtgcga gcgagggatg ttgaatcccc acggcccggg
     2821 gtaccggacc aattcgccct gatacacgtc ttccggcggc ctcacgagca cctccccgaa
     2881 aatcggaatc taacgttcca gatggtttcg aaccctttgc gtgatcgcct cggctattcg
     2941 cgaaagatag aattcagcct cttccgcgga agccttgcga gcgtcttctc ccaattgagc
     3001 gaaaagcgga tgctgcgtgt cgaaaaccac ccctctgtac cgtctttcct ccggaggcgg
     3061 tgtcgcatta ggccacgcct caggcccgcg gccttctctc agcgcccctg tgtccacaag
     3121 gtccgggaga agagcgagcc cgtaggacgt ctcccaccgt gcggcatggt cacccggaac
     3181 gtcgcgcagc ccctcgcctc ctatggtgca ttcggttccc cagatgaaaa gcacctcagg
     3241 atgctcgtca cggaccgggg acaacacctt gtccagaact ccctgccagg gataatgtcc
     3301 cgcatggagg acgatcgccc ggaaaccgaa gtcgatcagc tttcggacgg ttacgtcgag
     3361 aactcgttcg ccaaccacag ggtcctggta gaaggtccat ttgaagtcgc cgtgtccgcc
     3421 atggctgccc caccacaggc atggcagaac tacgccgcct gttgctgccg cgatgcgctc
     3481 gcagaaacgt acagccttga cgccgtcgaa gcccaccggc aacgcccacc cgtggtgctc
     3541 aatgagcccc agtggccagt acgccaccgg ccaccgttcc acgatttgct ccaattgatc
     3601 gggacgcagt tcctcaaagc gcccccacac gggcttattt cccatcctgc caactccctg
     3661 ccctgaacgt tccgtttgtt cttctgagtc aacctcggtc ccgcgcgttc gccttccggt
     3721 accggggacc ttgcgcgtac cggccggaac aggaaccgac cgaccgcggc ttcaacgtaa
     3781 aatatggtga gcgccgcgtt gcggacaacc ggaactgcgg ggaatgcgcg tggttgaggt
     3841 ttgcctgtgc tgccgagggc cgcctgtcaa cgccgctcca gcaaacgcaa cttgcgttcc
     3901 acggtgcgat ttgccggatc gagcctgaga gctgcgcggt acgcctcgcg tgcgttgtca
     3961 cggtctccga gcgcggacaa cacatcgcca aggtgggcat gcatttccgc aaccgagtca
     4021 tcaatctcga tggcgtcggc aagatgctgt ttcgcttcct ggtaacgccc ctgccggaag
     4081 aacagccatc ccatgctgtc aaggaacgcc gcattccttg gctcgaggcg cactgcttcc
     4141 gtgagaagag attcggcctc cgcgaggtga acgtttcggt cggcaaacat gaagccaagg
     4201 tagttgagcg cgtccgcgtg atgcctgttg atggcgagga gcctcttgaa cactaccact
     4261 gcgctgtcaa actcgccggc gcgctcgaga cccgcgccca ggtcgaacag ccgatcggtg
     4321 tcggtagtgt cacgttctgc cagtgcccga aggaccggta ctgcttcccg ccacttgttc
     4381 tgggaggaaa gtgcggcgct gtaaagggaa aggaggcgtt cgtcgccggg atattttgag
     4441 agcccctgcc gtgcagctcg ctcagcagca gcgtattgct tgaggcctgc ctgtgcgtag
     4501 gccagttcgc tcagcgcctc gagagatgaa tcttgacttg ccgcctgttc aaaggctgcc
     4561 gctgccgcgt gaaggtctcc ctgagcagca tgcgcctgcc cgagaagaac gtacccgtca
     4621 ccatcggaag ggtgcgtctc gatgagtcgt tgcgcgttcg cgattgccag gggaatctgt
     4681 ccccgtttgg tgagatacgt aatccagcct ttttgctcga cgagatcatc cggggattct
     4741 ctccgaatcc gcatccagac cgaatctgca tccgcccagc gatcctgggc gacgagcaac
     4801 tggatgtatc gtttgggcac ttcgcgggaa tccgggtggc tggcagacca ttcccgcagc
     4861 agtcgttctg cctccacgcg gcggccgctc cgggcaagat actccgcata cgcccctatc
     4921 ccctcttcat tacgcggcca ccgacgcatg cacgtcgtgt agaagatatc agaggcaccc
     4981 ttgaccggcg acatctggac ctgttccgcc catgccagca cgtcgtcagg atgtgcggtg
     5041 ggagaatccg ccatcgtctg gtacacacgg cggctttctt cgggcttcgc cagacgcgcg
     5101 tacaaacggg caagctgcca tcccgtttcc ggagatttgg actctctgaa ggcaggctca
     5161 aggtgcgtcg cggcgctcgc gaaatcgttg cggccctcgt acaagccggc aagaaaaaga
     5221 tgcccatcca cgtagtttga gtcgagcaga acacttcttc gaccgttcag aagcgcaagt
     5281 gaatcgttcc tctgcacgga gtacgccttc gccaacccca gccggagtga tggggacgag
     5341 gaatcagccc gaactgccgc ttgccactgc gcaagtgccc cggccgaatc tccccgacgt
     5401 tctgcgcgtt tggcggccaa cagccggaga atggcctcgg ggcgagggct ttgctgttgg
     5461 acagtcgttt cgtgaggcgg cgcatccggt acctcaagcg aggcgcagga actcattgcc
     5521 agcagggcac atgccgcaaa aaaggcttcc acacgcatcg aactgaattc ccttccattg
     5581 cgcgtccttt tccgtgtcat tcactgaaaa ggtacgcaaa acctgtgctg aggggtaaaa
     5641 aaaagaccgc ccacccgcaa agggtggcgc gatctttctg agctcttggt caaaagttca
     5701 ggtttcgagt ggttcgatgc ttaccacttt tcccttaccg ttcttatggg gggcgaactt
     5761 tacgtgccct gttgccagcg cgaagagcgt atcatcaccg cccctgccca cgtggagacc
     5821 cggatggaac cgggtgcccc gctggcgtac gatgattgtc ccggcattca ccagctcgcc
     5881 gccgaagcgc ttgagaccga gacgctgcgc attggaatcg cgcccgtttc gagaacttcc
     5941 gacgcctttc ttatgtgcca ttcgaagaac ctttccaagc agagcctatg cggccacgat
     6001 ttccttgatg agcacttgcg tataagactg acggtgcccc cgaaggcgcc gataccgttt
     6061 ccgccgcttc ttcttgaaca cgagaacttt cgcatcgcgc gcatgatcca gcacttcggc
     6121 ttgcacgctg gcaccttcga ccaccggttt gccaacggtg atcttgtcgc catcggaaag
     6181 aagaagaacc tctcccaggg tggttacggt gccgatttct ccgggaatcc gcgggaggaa
     6241 aatcttgtct cccggcgcca ctctggcctg cactccagca actcgcacta ctgcctgcat
     6301 gattttcgcc tggtttcggt ttcgtatgtt gggaaaaaag aacgggtcaa aagtactaga
     6361 ctttttaagt taccacccgt tggagcggct gtcaagcctc gtatttacct gtcaggtcct
     6421 ccgcggtttt caaactggag aagcggtacg cgtcgatctc cagcgaaggg ttgtcctcga
     6481 gaatgatctg agcgcccgtg gttcgccgca gggctttgag gatcctctgg tcgttttcac
     6541 gcagatagga gccaaccgtg ggatgaactt cgagaaccag tttcttctcg cctccggcgg
     6601 ctcgcagcct tttcagccac cgctcgagct tcgtcaccgt cgtgtcagga ctctgcacgc
     6661 gacccgatcc cttacagagc ggacacggtt cactgaacgt gtaaagcagg ctcggacgga
     6721 cccgttgccg ggtcatctcg agaacaccaa actcgctgag tcgttccgcg acattgattc
     6781 gcgaacggtc atgccgcagc gctttgttga actcttccca cacctttttc cggtttgctt
     6841 caatcgccat gtcgataaaa tcgatggcga tgatgccgcc gatatcgcgg aggcggagct
     6901 gtcgcgcgat ctcctgccct gcttcgaggt tgatgttgac gatcgtctgt tcctggttgt
     6961 tcctccccac ataccgccct gtattcacgt cgatcgtaac catcgcttcg gtgtgatcta
     7021 tcacaaggaa cccgcctttt cgcaatggca cgcggcgatt cgtcgcgttc gcgatttcct
     7081 gctcaatgcc atatgcgtcg aacacgggtt ctttttgatc gtagagaagc actctatcgc
     7141 gcaactgcgg agcggtgcct ttcaggtatt tgatgatatc gttgtacacg ctcttcgagt
     7201 ccaccacaac tcgttccacg tcttcgctga gaagatcgcg catcagactc gtcgtcattc
     7261 cgagttctga gtgaatgagc gccggcgcct tcattcgttt cgcgcggcga tggacccgtt
     7321 cccacgtgcg cttcagatca tcgatatccc gcgtcaggtc gtcttcgccc tgtccctctg
     7381 caaccgttcg cacaatgatg ccgcagtttt ccggacgaag gcgcgcggcg agcgctttca
     7441 gcctccgttt ttccgaccag ctcagaatct tgcgggacac gcccacgtgg ttggaatacg
     7501 gcatcagaac ggagaaacgt cccggcaacg atacgtcggt tgtgacacgc ggcccctttg
     7561 tgccgatggg ctcttttgtg atctgcacga tcacttcctg cccttttgtc aggagttcct
     7621 gaatcggcgt gtattttttt gtctcgcggc ctgatcgcgg ggcttcctcg tcgtcgtcct
     7681 cctcgtcttc gtcttcttcg tcgtcgagcc gttccggaga accaccaacg tctcttgcgt
     7741 gcagaaaggc gcttttttcc atcccgatat ctacaaatgc cgcctgcata cccggcagaa
     7801 tcgatgtgac gattcccttg taaatgttgc caacgaggcg ctgcgtatcg gggcgttcta
     7861 cgaggatttc caccaggcgt ccgtcttcga ggatagcgat acgggtttcc tgggtagcag
     7921 aattgacgat gatctctgtt ttcaacgcgt tccgtctttc cgttcactgg aaccacggac
     7981 acggcgaccc gtttccagtg gtccggcatc gaacgaggtt ccgtgatcag acggacggct
     8041 ggaaacagag aacggggagc ggcgcgaatg gaccagaata ccccggcggc tgagcagccg
     8101 gagggcgcat cgggccgtat ccgggccttg aaaggcccct gatcccgatt tcacgccgtc
     8161 gacagggtat tcccgccatc aaaacgcgac aacagaactg cgctcggagc gcgccatcgc
     8221 cacaggt
//
LOCUS       DAOTNG010000125         8214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7807_length_8214_cov_4.078318, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000125 DAOTNG010000000
VERSION     DAOTNG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_8214_cov_4.078318"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="P5179_07415"
     CDS             <1..1397
                     /locus_tag="P5179_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS95081.1"
                     /translation="TLNTSDDTWVQRTNNEFLGVNTLLRVSSVEPSVLKWTASYCYLH
                     TNNHLPGNSAYHDLPNTARLVSGQHLAEGMVRIPLPLLTEAEIRGYGSSRRDRFENKD
                     GTVGLGRSVTNDRTAAWGGQLTLSSLIVPLQRLSLFSQVAHEAYYPDDRMITDPAVRQ
                     KLLRSSRRAQYSFYLSDEIALLQRRIVLTGNVGTHTVSNNLIREETGSRTWSDSTSFT
                     QHPHAVGIVVAPTPWVSIRANLGRYTRVPNLFELYGNRGTSVGNSLLVPERGENRDIG
                     FVAHTTREDGRLRHASAEVVLFESKITDMIAFWSVYNRMKPFNVGKAQIRGVEVAGSL
                     ATAFGFSSNLGVTWQHPRNESALYNGLYFGNDLPHRPRWQIDSRSEFSFSGVTLAYGL
                     HYHNRFFGQPVNSIGDTMPSAFLHDVGVRFRPHERVHLSIEGKNLGNVREFHSRYVPL
                     PGRSVFVACQVSSS"
     gene            1432..2538
                     /locus_tag="P5179_07420"
     CDS             1432..2538
                     /locus_tag="P5179_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95082.1"
                     /translation="MLTKVVRRLIHTSATASILFVLASCGTNNPARSHSAGYGSAFVF
                     MTDYMSAAVGVFRPDSNSVDVLPLAGVHTDVALRSYGGLVYVLERFGKDAITVIDPAL
                     PTAPTANYSTGNGSNPQDIIVADAHRAYVLRLNTSNVLIINPQTGDSLGTIDLAPYAD
                     PADGRPEMAAGVLESGTLYVLLQLLDTASNPWLWPPTGFGKVVAIDTRTNAVTNAFTL
                     TVKNPVAMAVHNGTLFVAGGPYDDISATGIDRVPLSGGTPTRLVDGASLGGRPTALEV
                     CEQNNSAWVLVQKTWPTATVYRVSLTTGVTADSLAEPATPAAIAVGMDSVLLVSDRSM
                     SVPGLFVYNAITGEKRRGPIATALPPDAIVFVKD"
     gene            complement(2628..3920)
                     /locus_tag="P5179_07425"
     CDS             complement(2628..3920)
                     /locus_tag="P5179_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS95083.1"
                     /translation="MNVLLVAIDTLRADHLSCYGWNRLTSPHLDAFAAGGTVFLDMVS
                     PHIPTHPGYTTMFSGHDAMSHQVISHGGNVEPATGVKMIAEILGEQGYFTGAADNLGR
                     WFERGFQDYRRYSWVSNGTEPQRKAEAVNDVALPLLQDAANQDKPFFLFLHYWDPHTP
                     YLPPPPFSRMFFEGDEKDPADRSMDEVFAFAPFEKYFRAWLPGVTSREFVAAQYDAEI
                     AYNDAVLAHVFCRLRELNLEGETLVIVTSDHGETMDEHGCFFDHHGLYDANVCVPLIL
                     QCPGKIPAGQRLRGQVATFDIAPTILDYLGLGDLIAENAMIGTSLRPLAERGSQKGNY
                     DALYVAECSWMRKHGIRTNDWKYIEALEPDFHGLPPVELYDLNEKPVKETTNLVDARP
                     DVVADLKGVLEDWITRRPAQTGRPNPIMEQGITMRSVG"
     gene            4237..5622
                     /locus_tag="P5179_07430"
     CDS             4237..5622
                     /locus_tag="P5179_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95084.1"
                     /translation="MPEEPTSLLGRHRFVVSLSTVLGRSLSPDYGNGIVGESYAAGLG
                     EGATVGWDYGVTGVWALGAGQTLRWVNPDCFYRVSPSPKIGAMALSHAWERGSWSIGG
                     TAKRHSFSLTDDFQFVRFPRSSNVQINSFFYDHLETAIGRTISYDALGNNLMFVGDTR
                     WKTRFGTFGVMLSAHNASLDAVTEHRNTIADTLYSRHLLNGIKNGDLNGTWSGTGATL
                     AWDGTFRPGWSYGFRCGESQTSGDAVMWIRNPSEFPDGHGYTYKLEPRVWGLDTLSMK
                     NRHFSLRTSFDPAVSTGIECLFSWQGGAMANGAFGQTPVLTIDAVGDTVLPVSQGAFA
                     TVQGSFEGISILATIRHTPGNEPWSVEGSVGYVRVSAHADFSAVPRTTGFDLSSRTTT
                     WDLKAARFGYCAFGFTRRVTSQFYLTYSVRHLFPFSMDWHHNGARDSAPGGLTGFRFG
                     SLHEMSARMVL"
     gene            5722..7815
                     /locus_tag="P5179_07435"
     CDS             5722..7815
                     /locus_tag="P5179_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95085.1"
                     /translation="MTSSVSKAVAALFLAVFSIFGVASAGVIRDGVLMVDGKPFFPLG
                     SWNFSITTPEDIARLGMNVSYRGGPSTPEAVEQFRPFMRQCNRLGIQVVPYLSYGGAG
                     VIPWAPEAVRAISRLASEPNLLLWYVGDDITTRHLEGIQQTVSILRQEAPSVPTAADY
                     IAKATPEAKTTFTQFVDIRCQYSYPIPNDSLRSYMRFFDEQREFVGDPLWTWVQNFMW
                     ASTGVSLGLGIEDGPGPIPDPEQVRLLAFAAINRGVRGLLFFPHHELHLQPELAAEVA
                     LTCREIGLVSSHLAAAKTTFDLPTSDPDLNATAFRYGNSTVVSAMLARGHYHRWVDEA
                     VVKNVWIEVPWSGRGSSLPTAVLVATPDAVVCAVSPAQTPGWVRVRVPSLELAGFILL
                     STDSREIAQLRRGVQRITEQLSGLAVAGSIAQTRKVSAAAWSIGFGNLYDAGNLVLPA
                     VRLNEQAMDAVKEGDEVAEVRLWREANRVCRTVLDSMMVFAEARRALVPAAQQRYLNS
                     PYGLYAIKNLMRAPAVDDPWHHVAQWEVTGPFPLEYDQNAPDVFPPGFERVYPPENAT
                     ASSGPFATVDGPASWKPAVADITGITDFLNYFSTTENVVCYARTTVTAPRDTTMEMSI
                     GSNDGAKVWVNGTEVLSIHPPGGRAAAPHQNKVTVNLRAGRNVVLAKVENLGLNWQLY
                     LSFRDENRVLKFSTD"
     gene            complement(7908..>8214)
                     /locus_tag="P5179_07440"
     CDS             complement(7908..>8214)
                     /locus_tag="P5179_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95086.1"
                     /translation="LGIGALVMAILTFLQYRVPGWPLHPIGFPIAAAGNIRMMFFSIF
                     LTWVIKSILLRVGGVEAYERARPAFLGIIAGYALGVFISFFVDWIWFPGAGHQIHNW"
BASE COUNT         1697 a         2440 c         2334 g         1743 t
ORIGIN      
        1 cgactctcaa tacgtctgac gacacctggg tgcaacggac gaataacgag tttctcggtg
       61 tcaacacgct tctgcgcgtt tcctctgttg aaccatcggt actgaaatgg accgccagct
      121 actgctacct gcacacgaat aatcacctgc cgggaaacag cgcctatcat gacctgccca
      181 acaccgcacg cctggtttcc gggcagcatc tcgccgaagg gatggtgagg atacccttac
      241 cacttctgac ggaagcggag atccgggggt acggttcatc ccgtcgtgat cgttttgaga
      301 acaaagacgg aacggtcggt ctgggcagaa gcgtcaccaa cgaccgtacc gctgcatggg
      361 gggggcaact taccctgtca tcgctcatag ttcccctgca gcgtctttcc ttgttttccc
      421 aggttgcgca cgaggcttac taccccgatg acaggatgat cacagaccct gcagtccggc
      481 agaagttgct ccgttcgagc cggagggcgc aatactcctt ctacctctcc gacgaaatcg
      541 ctttgctcca gcggagaatt gtacttacgg gcaatgtcgg aactcataca gtcagcaaca
      601 atctgattcg ggaggaaacg ggctcgcgaa cgtggtccga ttccaccagt tttactcagc
      661 atccgcacgc agtcggcatt gttgttgccc cgactccctg ggtttcgatt cgcgccaatt
      721 tgggacgcta cacccgcgtt ccaaaccttt tcgagcttta cgggaacaga ggcacgagcg
      781 taggaaactc cttactggtt cccgaacgag gcgaaaatcg cgacataggt tttgtcgcac
      841 acacgacccg cgaagacggt cgccttcggc atgcctccgc ggaggtggtt ctctttgaga
      901 gcaagattac cgacatgatc gcattctggt cggtgtacaa ccgcatgaag cccttcaacg
      961 tggggaaggc acagattcgc ggggtggaag tagccggttc cctcgcaacc gcattcgggt
     1021 tttccagcaa tctcggcgta acgtggcagc acccgcgcaa cgaatccgcg ctctacaacg
     1081 gcctctactt cgggaacgac ctgccccacc gaccacgatg gcaaatcgat tcgcgcagtg
     1141 agttctcgtt ctctggcgta acgctggcgt atggacttca ttaccacaac cgatttttcg
     1201 ggcaaccggt taacagcatc ggtgacacga tgccgtctgc gttcctccac gatgtgggag
     1261 ttcgtttccg tccacacgaa cgagtgcatt tgtcgataga aggcaagaac ctgggaaatg
     1321 ttcgggaatt tcacagtcgc tacgtgcccc ttccagggcg aagcgtcttc gttgcctgtc
     1381 aggtttcttc gtcctgacag agcgttctgt tcgttaccaa aaggaggtcc catgctcaca
     1441 aaggttgtac gccgactcat tcacacgtcc gcgactgcct cgattctttt cgtgctcgcg
     1501 tcatgcggaa cgaataaccc tgcccggtcg cacagtgccg ggtacgggag cgcgttcgtt
     1561 ttcatgaccg attatatgtc cgccgcggtt ggtgtttttc ggcctgattc gaactccgtc
     1621 gacgtcctcc cccttgccgg cgtgcatacc gatgtcgcac ttcgatcgta tggcggactg
     1681 gtgtatgtgc tggaacgttt cggtaaggac gcgatcactg tcatcgaccc cgcgttgcca
     1741 acggcaccga cagcaaatta cagcaccggg aacggttcta atccccagga tatcattgtt
     1801 gcggatgcac acagggcgta cgttctgcgc ctcaatacaa gcaacgtcct catcatcaat
     1861 ccgcagacgg gggacagcct cgggaccatt gatcttgcgc cgtacgcgga ccccgcagac
     1921 ggccggcccg agatggcggc gggagtcctc gaaagcggaa ctctgtacgt tctgctccag
     1981 ttgcttgaca cggcctcgaa cccctggctg tggcctccaa ccggcttcgg gaaagtcgtt
     2041 gccattgata cgcgtaccaa tgcggtaacg aatgcgttca cgctgacggt gaaaaaccct
     2101 gtggcgatgg ccgtgcacaa tggcacgctt ttcgtggccg gtggcccgta cgacgacatc
     2161 agcgcaaccg gaattgacag ggtaccttta tccggaggta cgccgacccg ccttgtggac
     2221 ggcgcttccc tcggggggag accaaccgca ctggaggtgt gcgaacagaa caactccgcg
     2281 tgggtactgg ttcagaaaac ctggcccacg gctaccgtgt atcgggtgag cctgaccacc
     2341 ggtgtcacgg cggatagcct cgccgaaccg gcgaccccgg ccgccatcgc cgtaggaatg
     2401 gacagcgtgt tgctggtaag cgatcgctca atgagtgttc cggggctttt cgtgtacaac
     2461 gccatcactg gagagaaacg acgaggtccg attgccacgg cgctgccgcc ggacgcgatc
     2521 gtttttgtca aggattagtg attggttatg aaaacgggcg agccgggagc ggaacgatgc
     2581 atgtgcctcg catctccacg caaccgcttc cggccctgcc cctccaccta cccaactgac
     2641 ctcatggtga tcccctgttc catgatcgga ttcggccttc ctgtctgcgc cgggcgacgc
     2701 gtgatccagt cctcaagaac tcccttcagg tcagcaacca cgtctggccg tgcatccacc
     2761 agattcgtgg tctctttcac tggtttctcg ttcagatcgt aaagctctac cggcggaagc
     2821 ccatggaagt cgggttccag agcctcaatg tatttccagt cgtttgtccg aattccgtgt
     2881 ttccgcatcc atgagcactc agctacgtac agagcgtcgt agttcccctt ctgggagccc
     2941 ctctctgcaa gaggtcgcag actggttccg atcattgcgt tttccgcgat caaatccccc
     3001 agcccaaggt agtccagtat cgtgggcgcg atgtcaaacg tggccacctg ccctcgcaag
     3061 cgttggccgg cgggaatctt gccgggacat tgaagaatga gtggaacaca tacattcgcg
     3121 tcatacagcc cgtgatggtc gaagaaacac ccatgttcgt ccattgtctc gccatggtcg
     3181 gacgtcacga taacaagcgt ctccccctca agattcagtt cacgtagccg gcaaaacaca
     3241 tgagcaagta ccgcatcgtt gtatgcgatc tctgcgtcgt attgcgcagc gacaaactcc
     3301 cgacttgtta ctcccggcaa ccacgcacgg aagtatttct caaaaggtgc gaacgcaaac
     3361 acctcgtcca tggaccggtc cgcagggtcc ttctcgtcgc cttcgaaaaa catgcgcgag
     3421 aatggagggg gaggcaggta gggtgtgtgg gggtcccagt aatggaggaa aaggaaaaag
     3481 ggtttgtcct ggttcgcagc atcctgaagg agcggaagcg ccacgtcatt caccgcctcg
     3541 gctttacgtt gcggttcagt tccgttcgat acccaggagt acctgcggta atcctggaat
     3601 ccccgttcga accagcgccc tagattgtcc gcggcaccgg tgaaataccc ctgctcgcca
     3661 agtatctcag caatcatttt cacaccggtc gcaggttcca cgtttccgcc atgggaaata
     3721 acctgatgtg acatggcgtc gtggcccgaa aacatcgtcg tgtaacccgg gtgcgtcgga
     3781 atatggggcg acaccatgtc aaggaacacc gtgcccccgg ccgcaaatgc atcaaggtgg
     3841 ggactggtaa gcctattcca gccatagcaa cttagatgat cggctcggag agtatcgatc
     3901 gctacaagaa gaacgttcat agtcgctcct ttcgacattt gcgcactttc aacccgggga
     3961 tctcgcgcga tttcccgcgt gtacgattta cgtccgccgt ggactcgagt caacgggtta
     4021 tggccgcgga atgcacgcag aaacccaccg acgtacattt ggaatcgtca aaatagtgtc
     4081 agctggatgg cggggtctga acgcgtgcgc ggaacactcc tgttactttt cccggtgttg
     4141 gttggagtta caaaaacttc cgccggcctc ggtccaatga acctgctcgt tgatgggctt
     4201 ggtggcgtcc ggacgagtcc ccgaagcgag ttgtggatgc cggaagaacc gaccagcctc
     4261 ctgggcaggc atcggttcgt cgtatcactc tcgaccgtac tcgggcgttc tctttccccc
     4321 gattacggta acgggattgt cggggagtcg tacgccgcgg gtctcgggga aggcgccacc
     4381 gtgggctggg actacggggt aaccggcgtc tgggcgctcg gtgccggaca gactctccgg
     4441 tgggtcaatc cggactgttt ctaccgcgtg tctccctcac cgaaaattgg agcgatggcg
     4501 ctctcccatg cgtgggaacg cggttcctgg tctatcggcg gcaccgcgaa acgacactcg
     4561 ttcagtctta cggatgactt ccagtttgtg cgtttcccac gctcgtccaa tgtccagatc
     4621 aacagtttct tctacgatca cctcgaaaca gcaattggac gaacaatctc gtatgacgcg
     4681 ctcggcaaca acctgatgtt cgttggcgac acgcgctgga aaacacgttt cggtaccttc
     4741 ggcgtcatgc tgtccgcaca caacgcaagt ttggatgccg tcacggaaca cagaaacacg
     4801 attgcagaca ccctctattc ccggcacctg ctgaacggca taaaaaacgg tgatctgaac
     4861 ggaacatgga gcggcaccgg cgccacactt gcctgggatg gtacgttccg gcctggttgg
     4921 tcgtacggtt tccggtgcgg cgaaagccag acctccggtg acgcggtgat gtggatccga
     4981 aacccgtctg agttccccga cggacatggg tacacgtaca aactcgaacc ccgggtgtgg
     5041 gggttggata cgctgtcgat gaagaacagg catttctcgc tgcgcacgtc gtttgatcct
     5101 gcagtgtcga ccgggattga gtgcctgttc agctggcagg ggggggccat ggcaaacgga
     5161 gcgttcggcc agacacccgt gcttactatc gacgccgtag gcgacactgt cctgcctgtc
     5221 agtcaggggg ccttcgcgac cgttcagggc tcatttgaag gcatttcgat acttgccacc
     5281 atccggcata ctcccggcaa cgaaccgtgg agtgtcgaag gaagtgtcgg gtatgttcgc
     5341 gtttccgcgc atgccgattt ttccgcggtt ccccgtacaa ccggattcga tttgtcctcg
     5401 cgtacgacca catgggacct gaaggctgcg cggtttgggt attgcgcgtt cggtttcacc
     5461 cgccgcgtaa ccagccaatt ctacctcaca tattccgtgc gccatttgtt cccattttcc
     5521 atggattggc atcacaacgg ggcgcgtgat tcggcacctg gcggcctgac cggtttccga
     5581 ttcggaagcc tgcacgaaat gtccgcacgc atggtgcttt gatcggccca ggtcggcccc
     5641 ctcccttggg gcgtttggag ctccctcgcg acatttcaat tgcccctcag gtatcttccc
     5701 atggcaatca aggagagtcc aatgacgagt tcggtatcaa aggcagtcgc agcgctgttt
     5761 cttgccgtgt tttccatatt tggcgtcgcc tcggcgggcg tcattcgaga cggcgtgctg
     5821 atggtggacg gaaaaccgtt cttccccctc ggaagctgga acttttcgat tacaacaccc
     5881 gaggatatcg cgcgtctcgg tatgaacgta agttaccgag gcggcccatc aactcccgag
     5941 gcggtggagc agttccggcc tttcatgcgc cagtgcaaca ggctgggcat ccaggtagta
     6001 ccatacctct cgtacggcgg cgcaggcgtc atcccatggg cgccggaagc tgtccgagcg
     6061 atttccagac tggcgagtga accgaatctt ttgctctggt acgtgggtga cgatattacg
     6121 acgcgacacc tcgagggcat ccagcagacg gtgtctatct tgcgacagga agcaccttcg
     6181 gtgccaaccg cggcagatta catagccaag gcgacaccgg aagccaagac cacattcacg
     6241 caattcgtgg acattcggtg ccagtattcc taccccatcc caaatgactc cctgcggtcg
     6301 tacatgcggt tctttgacga gcagcgggag ttcgtgggag acccactctg gacatgggtg
     6361 cagaacttca tgtgggcgag caccggagtc tcgctgggac ttggcataga agacggaccg
     6421 gggcccattc cggaccccga gcaggtgcga cttcttgcgt tcgctgcgat caatcgcggg
     6481 gtgcgcggcc ttctgttctt cccgcaccac gaacttcacc tgcagccgga gctcgccgcg
     6541 gaagtagcct tgacgtgccg tgaaattgga cttgtgtcgt cccacctggc tgccgcaaaa
     6601 acgacgttcg atctccccac ctcagacccg gatctcaatg caaccgcatt ccggtacggc
     6661 aattccacgg tcgtctccgc aatgcttgcc cgcgggcact atcatcgttg ggtagacgaa
     6721 gcggtcgtga aaaacgtctg gatcgaagtg ccttggagcg gaagagggtc ttcgctccca
     6781 acggccgtac tggtcgccac ccccgacgca gtggtttgcg cggtttcacc tgcccagaca
     6841 cccggctggg tgagggtcag ggtgccatcc cttgaactcg ccgggtttat cctgctgtca
     6901 accgattcgc gcgagatcgc gcagctccgc cgcggcgtgc agcgcatcac ggaacagctc
     6961 tcggggctcg cggtcgcggg atctatcgct cagaccagaa aagtctcggc ggcggcgtgg
     7021 tcaatcggtt tcggaaacct ttacgacgcg gggaatctgg ttctgcctgc agtccgtctc
     7081 aacgaacaag cgatggatgc agtcaaagaa ggtgatgaag tcgccgaagt gcgtctgtgg
     7141 agagaagcaa accgagtctg caggactgtt ctcgacagca tgatggtgtt cgcagaagca
     7201 cgtcgagcgc tggtgccggc ggcgcaacag cggtacctca acagcccgta cgggctgtat
     7261 gcgatcaaga atctcatgcg tgcgcctgcg gtggacgatc cgtggcatca cgttgcgcaa
     7321 tgggaagtaa cagggccgtt cccgctcgaa tatgaccaga acgcgccgga tgttttccct
     7381 ccagggttcg agagggtgta tccacctgaa aacgctaccg cgtccagcgg accgtttgca
     7441 actgtggacg gtcccgcatc gtggaaaccc gccgtggcag acatcacagg aataacggat
     7501 ttcctgaact atttcagcac cacggaaaat gtcgtttgct acgcgagaac cacggtgact
     7561 gcgccccgtg acacgacgat ggagatgagc atcggcagca acgacggggc gaaggtgtgg
     7621 gtcaacggga cggaagtgct ttcgattcat ccccccggcg gccgagccgc tgcaccacat
     7681 cagaacaaag tgaccgtcaa cctgcgcgca ggacgcaatg tggtgctcgc aaaggttgag
     7741 aacctggggc ttaactggca gctctacctg tcattccgcg atgaaaaccg cgtgctcaag
     7801 ttctcgacgg actgagcaag ccattccgat cctcccaaaa ggaaccggcg tgtggctctt
     7861 caacgccaca cgccggtttt attcctccta tgcatcccca cgggtgctca ccagttgtgt
     7921 atctggtgtc ccgcgccggg gaaccagatc caatccacga agaagctgat gaataccccc
     7981 agtgcatagc cggcgataat gccaaggaac gcagggcgcg ctcgctcgta tgcttccacg
     8041 ccaccgaccc ggagcagtat gctttttatc acccacgtca gaaaaatgct gaagaacatc
     8101 atcctgatgt tgccggccgc ggcgattgga aaaccgatcg gatgcagcgg ccaacccgga
     8161 acacgatact gaaggaacgt caatatcgcc atgactagcg cgccgatacc aagg
//
LOCUS       DAOTNG010000126         8198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7828_length_8198_cov_5.292644, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000126 DAOTNG010000000
VERSION     DAOTNG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7828_length_8198_cov_5.292644"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="P5179_07445"
     CDS             complement(<1..565)
                     /locus_tag="P5179_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95087.1"
                     /translation="MQSPIPRSALFNFAAKVYPARGNTLDGVCDEWDTHHQLPDLALD
                     GEAFATVWMAWEPDALWFAVKVPRTRAPQVIPNRPASGDCVELYIDTRDVRSAHRAGR
                     YCHKFIFAPQGRAGRARHPFFEHAEIARATASPPKVSPDQVEIAASVDADSYSMEIRF
                     PAEALFGYDVETNRRLGLAYVITTSSGP"
     gene            789..2672
                     /locus_tag="P5179_07450"
     CDS             789..2672
                     /locus_tag="P5179_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95088.1"
                     /translation="MNEYRHVKMHLRLFASFLCAALCVACAGTHGAAPRPAPEYATPP
                     VRLVPPPEFRPDANPDRVGTILFVTISPLYLLEKAAYFGSNGVDGIMMSGLMGSWHSD
                     VWAANRYTDDPPGTRVVGAQNSLLKLCKRMNQRCRDAGVDYNSVQVSVAQPVPDWFDD
                     AGWARIVENFRQGAIFARDAGFAGISFDHEYISDWYLLRNPKYSEPGYPPEAEVRRMV
                     QQRGYEIMSAMLDEFPDMIFWLLPENPDNYEGWLGVDMFVGLTRGMAERDAPGGLHLC
                     TERSYLNLRPRGLATINNWTSESARFALRKAATPEVLDYWMRRCGLSVGIWPLGFYYE
                     FKDSTGKPYGYTGKRSTYPGEWVGSYADKTENYPVENFEWQFAGARSLSRRFVWLYCH
                     GTVLWRMTPEEMAHYRATPNDTLPTVWNLPEYLRILRERPVMQDPLFEATAASIREHR
                     GFPAFAGFVPRWWHVGPFPNFMENFDVMYPPERGVDLAAAYAGDPSYPDSLRVLRWRE
                     SSTDATGYVDLSALFSKPGLPLAYSVAYVESPRRQRVWFRFGSNDFGRVYVNGRQVHD
                     IREQRAAAIDDDNFEVELPAGRSEILVKCGDAGGKRWGFYLRLTDAAGNEVPGLKWVG
                     AED"
     gene            2902..4842
                     /gene="thrS"
                     /locus_tag="P5179_07455"
     CDS             2902..4842
                     /gene="thrS"
                     /locus_tag="P5179_07455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRS95089.1"
                     /translation="MVRVTLPDGTPLELHDGATALDAAEKIGPRLAKATLAAKINDLP
                     VDATTALRDGDRLALLTWDSPEGRDVYRHTTSHIMAQAVKRLFPEAKPAIGPALEDRF
                     YYDFEVGRPFSPEDLEKIETAMAEIIKEDLPVTRFELPVEEAIKLFSDRNEPYKVEIL
                     RDLSDQSELVPGEKVSLYQQGEYVDLCRGPHLPSTGRVKAFKLLSSSGAYWRGDEKRQ
                     MLQRIYGTSFPDRKQLESFLAVLEEAKRRDHRILGPRLDLFSIHPEVGAGLIHWHPKG
                     ALIRHLIETLWKEEHMRRGYSLVYTPHIASDNLYRISGHLEKYDAMYRPMDIDGENYR
                     VKPMNCPFHIMIYQSQTRSYRDLPIRYAELGTVYRYERSGVLHGMLRVRGFTQDDAHI
                     FCRPDQLVDEVSDTLDLMKFFLSTFGLDYQVYLSTRPERSFGTDEEWAVATDALKETL
                     EKAGVPYTVDPGEGVFYGPKIDVKLLDVLGRPWQGPTIQVDFQLPQRFGVEYVGSDNQ
                     THNAVMVHRAMLGSMERFVGCLIEHFAGAFPLWISPVQVVVLPLTEAQTDSAAELAGR
                     LKAAGLRVETDLRSEKIGSRIRDAELQRVPYMLILGAREVENGAVAVRARERGDLGAK
                     PVDEVIRLLCEEVSTRSLTPKV"
     gene            4800..5399
                     /locus_tag="P5179_07460"
     CDS             4800..5399
                     /locus_tag="P5179_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95090.1"
                     /translation="MRGSVNPFIDAEGMISGAVSVVRGQKRTSANRLRVNREISALRV
                     FLVGADGVPEGEVTREEALLRASARGFDLIEVQPGANPPVCRLGKWADTAAVSPPPVE
                     AEQRTLWVPVGCTQTDFRLKIAHGRRFLTEGGVLEVVVPVEGSRDKAAAEQMLRKVVE
                     AFSDCARPGAVKRSNTELTMVFSPVTSGETRADRPDTGE"
     gene            5414..5608
                     /gene="rpmI"
                     /locus_tag="P5179_07465"
     CDS             5414..5608
                     /gene="rpmI"
                     /locus_tag="P5179_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRS95091.1"
                     /translation="MPKMKSRRNARFRFKVTANGKILRHQSNCNHILTKKSSKRKRFL
                     RKSVLADAADVPRIRRMLLA"
     gene            5676..6029
                     /gene="rplT"
                     /locus_tag="P5179_07470"
     CDS             5676..6029
                     /gene="rplT"
                     /locus_tag="P5179_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRS95092.1"
                     /translation="MPRVKHGVASHARHKKALEHAKGYYGGRSKLFRTAREAVDRALA
                     YSYRGRKEKKRDFRSLWIVRINAAARMHGLSYSRLMYGLSQAGVDVNRRMLADLAVHD
                     PEAFGRLAETAKAQF"
     gene            6117..7142
                     /gene="pheS"
                     /locus_tag="P5179_07475"
     CDS             6117..7142
                     /gene="pheS"
                     /locus_tag="P5179_07475"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRS95093.1"
                     /translation="MTAELLDELDRIAAQAPARIDACASPEEVEALRVEYVGTKGSIT
                     RINKLLREVPADQRPGLGKRVGEVRSLVLQKLQEAGQKFQVEEGARRQKIDITLPGRT
                     FLLGRRHPLTIALDRITSIFSDMGFTVADGPEIESEYYNFEALNMPPDHPARDMTDTY
                     YVTPRWPLRSQTSPVQVREMEKRRPPVRIIVPGKVYRNEDVSARAMNQFFQVEGLYVD
                     RNVTFADLKGTLETFCKRFFDPKTRVRFRPSYFPFTEPSTEVDVSCILCNGSGCRVCK
                     YAGWLEILGAGMVDPNVFGFVDYDPEEYTGFAFGMGIDRTTMMRYGVDDIRHFWENDM
                     RFLSQFE"
     gene            7197..>8198
                     /gene="pheT"
                     /locus_tag="P5179_07480"
     CDS             7197..>8198
                     /gene="pheT"
                     /locus_tag="P5179_07480"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRS95094.1"
                     /translation="MRISLGWLNDFFPTDGLSAANIADRLTASGIEATPIGTLIPGDS
                     LVIGEVLTCEKHPNADRLSVCGVTTGGAPMTIVCGAPNVAAGQKVVIAPVGTELANGM
                     RIKRAAIRGVTSEGMICAEDEIGLGDDHEGIMVLTPDAPTGMSFLKYLHLDSERLELE
                     LTPNRPDAMSHRGVARDLSALFGRPTTNPITSPEENGRPVEEITSVLIDDPEGCPRYV
                     ARVVEGVRIAPSPEWLAGRLRQVGVRPINNVVDVTNYVLMAIGHPLHAFDLHRLAGRR
                     IVVRAAERDERFTTLDGTERIIPEGSVFICDGERPVALGGIMGGLNSEITDDTTDVLL
                     "
BASE COUNT         1662 a         2341 c         2593 g         1602 t
ORIGIN      
        1 atgggccgct cgatgtcgta atgacgtacg ccagtccaag ccgccggttg gtctccacgt
       61 cgtacccgaa gagcgcctcc gcgggaaagc gaatctccat cgaatacgaa tctgcgtcca
      121 cggacgcagc gatctccacc tgatcgggcg acaccttcgg cggggaggct gtcgcccggg
      181 cgatctccgc gtgctcaaaa aaggggtgcc gtgcccgtcc agctcgtccc tggggcgcaa
      241 aaatgaactt gtggcagtag cgcccggccc ggtgggcaga ccggacgtcc cgggtgtcga
      301 tgtaaagctc gacgcagtcg ccgcttgccg gtcggttggg gatcacctgc ggggcgcgcg
      361 tccgcggcac cttcaccgcg aaccacagcg cgtcaggttc ccaggccatc cacaccgtgg
      421 cgaaggcttc gccatccagc gcgaggtcag gcagttggtg gtgcgtgtcc cactcgtcgc
      481 atactccgtc cagcgtattg ccgcgcgccg ggtagacttt ggcggcgaag ttaaacagtg
      541 cggaacgtgg gatcggagat tgcatggcgg cttccgagag cttcgtgtgt tcgggcgcag
      601 gacatgccct gcgcccctca ggaagaagaa taccgtccgc cgcggggtgc acgcaattcc
      661 tggctggttg cgcccggccc cattgccgcc ggatattgtg tcttccacca ggcaaacttc
      721 agcatcgacc ccgtctggga atcctgccgt tccgacctgc caaccaccca cctttggaga
      781 ccgctcaagt gaacgagtat cgccacgtca aaatgcatct gaggcttttt gcgtcgtttc
      841 tgtgcgctgc cctctgtgta gcgtgcgcgg ggacgcacgg ggcggctccc cgacctgctc
      901 cggaatacgc cacgccgccg gttcgcctcg taccgcctcc cgagttccgt ccagacgcga
      961 accctgaccg cgttggcacc atcctgtttg tgacgatctc tccgctctat ctcctggaaa
     1021 aggccgcgta tttcggttcc aacggcgtgg acggcatcat gatgtcggga ctgatgggga
     1081 gctggcacag tgacgtctgg gccgccaatc gctacacgga tgaccctccg ggcacccgtg
     1141 tcgtgggagc acagaactcc ctcctgaagc tctgcaaacg gatgaaccaa cgatgtcggg
     1201 atgccggtgt ggattacaac agtgttcagg tctctgtggc gcaacctgtg ccggactggt
     1261 ttgacgacgc cggatgggcg aggattgtgg agaacttccg ccagggcgcg atcttcgccc
     1321 gtgacgccgg gttcgccggg atatccttcg accacgagta catctccgac tggtacctgc
     1381 ttcgtaaccc gaaatacagc gaaccggggt acccgcccga agccgaagtc cgcaggatgg
     1441 tgcagcaacg agggtacgag atcatgtcgg ccatgctgga cgagttcccg gacatgatat
     1501 tctggctcct tccggagaac ccggataact acgaaggctg gctcggggtt gacatgtttg
     1561 tcggcctcac tcgcggcatg gcggaacggg atgcgccggg agggctgcat ctgtgcacgg
     1621 aacggtcgta cctgaacctc cgcccgcgcg gcctcgccac gatcaacaat tggacgagcg
     1681 agtctgcgag attcgcgtta agaaaagccg ccacgcctga ggtgctcgat tactggatgc
     1741 gacgttgcgg gctcagcgtg ggtatctggc cgctcgggtt ctactacgag ttcaaggact
     1801 ccacagggaa accgtacggc tacacgggta aacgatccac gtatcccggc gaatgggtcg
     1861 gctcgtacgc ggacaagaca gagaactacc ccgtggagaa cttcgaatgg cagttcgccg
     1921 gtgcacgctc gctctcgcgc cgttttgtgt ggctctactg ccatgggacg gttctgtggc
     1981 gaatgacgcc ggaagaaatg gcccattacc gcgccacgcc gaacgacacg ctgccgactg
     2041 tctggaatct gccggaatac cttcgcatac ttcgggaacg ccccgttatg caggatcctc
     2101 tttttgaggc aacggcggcc tcgatacgtg aacacagggg gtttcccgcg ttcgcgggct
     2161 ttgtaccgcg ctggtggcat gtcgggccgt tcccgaactt catggagaac ttcgacgtca
     2221 tgtacccgcc ggagcgggga gttgaccttg ctgccgccta cgcgggcgac ccttcatatc
     2281 ccgattcgct gcgtgttctg cgctggcgcg agtcctcgac cgacgccact ggttacgtgg
     2341 acctctccgc gctgttcagc aaaccaggcc tgcctctcgc ctacagcgtt gcctacgtgg
     2401 aaagcccgcg caggcaaaga gtctggttcc ggttcgggag caacgatttc ggccgcgtgt
     2461 acgtgaacgg ccggcaagtg cacgacatcc gtgagcagcg agcggccgcc atcgatgatg
     2521 acaacttcga ggtggagttg ccggcaggac gttccgagat tctcgtcaaa tgcggggacg
     2581 ccggaggcaa gcgatggggg ttctatctgc gtctcactga tgccgcggga aacgaagtgc
     2641 ccggtctcaa atgggttggc gccgaggatt agataccttc ggcggtttgg caaatatcgt
     2701 gccgtggggg tatcttaata attcttgtgt tctcgcttcc agacggtggt acgtcgcatc
     2761 gccaggaagc acgcaatgcg ccggtgaggt gcctcaccat gtgagtgcgc tcgccggcgt
     2821 ttccatctgt ggatggctga cgcgagggct gcggtcgtct gtggcagggc gttggctgtt
     2881 cgtacggaaa ggagttcaaa tatggttcgc gtcactctcc ccgacggcac acccctcgag
     2941 cttcacgacg gtgcaacagc cctcgatgcg gcggagaaaa tcggtccgag gcttgccaag
     3001 gccacgcttg cggcgaaaat caacgatctt ccagtcgatg ccaccactgc tcttcgcgac
     3061 ggagaccggc ttgcccttct gacatgggat tcccccgagg gaagggatgt ctaccggcac
     3121 acaacttcgc acatcatggc acaggcggtg aaacgtctgt ttcctgaagc caaacccgcg
     3181 atcggtccgg cgcttgagga ccgtttctac tacgatttcg aggttggcag accgttctcg
     3241 ccggaagatc tggagaaaat cgaaacggcg atggcggaaa ttatcaagga agacctgccg
     3301 gttacgcggt ttgagctgcc ggtcgaggaa gcgatcaagc tcttcagcga ccgaaacgaa
     3361 ccctacaagg tggaaattct gcgcgatctc tccgaccaga gtgagctggt tcccggggaa
     3421 aaagtgagcc tgtatcagca gggcgagtac gtggacctct gtcgcggtcc ccaccttccc
     3481 agcaccggga gggtaaaggc gttcaaactg ctgagttcct ccggcgcgta ctggcgcggc
     3541 gacgaaaaac gccagatgct ccagcgcatt tacggcacca gctttccgga caggaaacag
     3601 cttgagagtt tccttgccgt gctggaagaa gccaagaggc gtgatcaccg gattctgggt
     3661 ccgcgcctgg accttttcag cattcatcct gaggtcggtg ccgggttgat tcactggcat
     3721 ccgaagggcg ctttgatccg gcacctgatc gagactctgt ggaaagaaga gcacatgcgg
     3781 cgggggtact ccctggtgta cactccgcac attgcgagcg acaatctgta ccggataagt
     3841 ggtcaccttg agaaatacga cgcgatgtat cgcccgatgg atattgacgg cgagaactac
     3901 cgtgtcaagc caatgaactg cccgttccac atcatgatat accagtcgca gacccgcagt
     3961 taccgcgacc taccaatccg atacgcggaa ctgggcacgg tgtaccggta cgaacggtcc
     4021 ggggtattgc acggcatgtt gcgtgtacgc ggcttcacgc aggatgacgc gcacattttc
     4081 tgccggccgg accaactggt ggacgaagtc tccgataccc ttgacctgat gaagtttttc
     4141 ctcagcacgt ttggtctcga ttaccaggtc tacctgagca cgaggcccga gcggagtttc
     4201 ggaaccgacg aggaatgggc ggtagccact gacgcactga aggagacgct cgagaaggcg
     4261 ggcgttccct acacggtgga tccgggtgaa ggcgtgttct acgggcccaa aatagacgtc
     4321 aaattgctgg atgtactggg cagaccttgg caagggccaa caattcaggt agattttcaa
     4381 cttccccaac gcttcggcgt ggagtacgta gggtcggata accagacaca caatgcggtc
     4441 atggtgcacc gcgccatgct cggttccatg gagcggttcg taggttgcct gatcgagcat
     4501 tttgccggtg cgttcccgct gtggatatcc ccggtgcagg tcgtcgtgtt gccccttacc
     4561 gaggcgcaga ccgactcggc ggcggagctg gcggggcggt tgaaagccgc cgggttgcgc
     4621 gttgaaaccg accttcggag cgagaagatc ggatcgcgga ttcgcgacgc ggaactgcag
     4681 cgcgtaccgt acatgctcat ccttggtgcc cgcgaagtgg aaaacggggc cgttgccgtt
     4741 cgtgcgcggg aacgaggcga tctgggcgca aagccggtgg atgaggttat tcgcctcctg
     4801 tgcgaggaag tgtcaacccg ttcattgacg ccgaaggtat gatttccggg gccgtttctg
     4861 tcgtccgcgg ccagaagaga acaagcgcca accgactccg cgtaaaccgg gaaatatcgg
     4921 ctctccgggt gtttctcgtc ggggccgatg gagtgccgga aggcgaagtc acgcgtgaag
     4981 aagcgctttt gcgagcctcg gcacgcggat tcgatctgat agaagtccag cccggggcaa
     5041 atccgccggt gtgtcggttg ggcaagtggg ccgatacggc ggcggtcagt ccgccacctg
     5101 tagaagctga acaacggacg ttatgggtcc cggtcggctg cacgcagacc gacttccggc
     5161 tgaagatcgc ccacggccgc cgatttctga ctgaaggtgg cgttctggag gttgtggttc
     5221 cggttgaggg ttcgcgggac aaggcagccg ccgaacagat gttgcgcaaa gtggtcgagg
     5281 ctttttcaga ttgcgccagg ccaggagcag tcaagagaag caacacggaa ttgacgatgg
     5341 ttttttcgcc ggtcacgtcg ggtgaaaccc gcgcggaccg gcctgatacc ggagaataga
     5401 gggagagcaa gctgtgccaa aaatgaaaag ccgccggaac gctcggttcc ggttcaaggt
     5461 gaccgcgaac ggaaagattc tgcggcacca gtcgaattgt aatcatattc ttaccaagaa
     5521 atcgagcaag cgcaagcgtt tcctgcgcaa aagcgtcctt gctgacgcag cggatgtgcc
     5581 acgcatccga cggatgctgc tcgcgtaatc agtttcatac gggagctgtt tccaccgggc
     5641 aggtggccgc agcgattatc acacggagtc aaaacatgcc acgggtcaaa catggagtgg
     5701 cgtcgcacgc acgtcacaag aaagcgctgg agcatgcgaa agggtattac ggcgggcggt
     5761 cgaagctgtt ccgcaccgca cgtgaagcgg tggaccgcgc actggcctat tcgtatcgcg
     5821 gcaggaaaga aaagaaacgg gacttccgtt ctctctggat cgtccgcatc aacgcagcag
     5881 caaggatgca cgggctgagc tattcccggc tcatgtacgg gctgtcgcag gccggtgtag
     5941 acgtgaatcg gcgcatgctt gcagacctgg cggtgcacga tcccgaggcg ttcggccggc
     6001 tggcagaaac cgcaaaagcg cagttttaac ctccacggaa ggagaggcgg acaacgcctt
     6061 gtcaagagcg cgcggaacga tgcgctcctg acgagaacgt ccgccttttt tgcatcatga
     6121 cagcggaatt gcttgacgaa ctcgacagga ttgccgcaca ggcccccgca cggattgacg
     6181 cgtgtgcgtc gcccgaagaa gtggaagcgt tgcgcgtgga gtacgtggga acgaagggca
     6241 gtatcacgcg catcaacaaa ctccttcggg aagtgcctgc cgaccagcgg ccaggcctcg
     6301 gcaaacgcgt gggggaggtg cgttcgcttg tactgcagaa gttgcaggag gccggccaga
     6361 agtttcaggt cgaagagggt gcccgcaggc aaaagatcga cattacttta cccggccgga
     6421 cctttcttct gggccgccgc cacccgctca cgatagccct tgaccgtatc acgtcgattt
     6481 tctcggacat ggggttcacc gtggcggacg ggccggagat cgaaagcgaa tactacaact
     6541 tcgaagcgct caatatgcct ccggatcatc cagctcgtga catgacggac acctattacg
     6601 tgaccccgag atggcctctg aggtcgcaga cttcgccggt gcaggtgcgc gaaatggaaa
     6661 agcgacgtcc accggtgcgc atcattgttc ccggcaaggt gtaccgtaac gaggacgtca
     6721 gcgcccgggc catgaatcag ttcttccagg tggaagggct gtatgttgac cggaacgtca
     6781 cgttcgcgga cctcaaggga acactggaaa cgttctgcaa acggttcttt gacccgaaaa
     6841 ccagagtccg tttccggccg tcgtatttcc ctttcacgga acccagcacg gaagtggacg
     6901 tgagctgcat cctctgcaac ggttcaggat gccgggtgtg caaatacgcc gggtggctgg
     6961 aaatactggg cgctggaatg gtagatccta acgtattcgg ttttgtggac tatgacccgg
     7021 aagagtacac cgggttcgcg ttcggcatgg gcattgatcg caccacgatg atgcggtacg
     7081 gggtggacga tatcaggcat ttctgggaaa atgatatgcg attcctgtcc cagttcgaat
     7141 gagaacggtg gacgcgtgaa gggcgacagg ggtcaacttg acgaaggcgc gagagcatga
     7201 gaatctcact tggttggctg aacgacttct ttccaacgga cgggttgtcc gcggcgaaca
     7261 ttgctgatcg cctgactgcg tccggcatag aagccactcc gatcggcaca ctgattccgg
     7321 gggattccct tgtgatcgga gaggtgctga cgtgtgaaaa gcacccgaac gcggaccgcc
     7381 tgtccgtatg tggcgtgacg actggcggtg ctcccatgac gatcgtatgc ggcgcgccga
     7441 acgtggcagc aggccagaaa gtcgtcattg cgccggtcgg gacggaactc gccaacggaa
     7501 tgcgcatcaa aagggccgca atccgcggtg tcacttctga aggcatgatc tgcgcggaag
     7561 atgagattgg cctcggtgac gaccacgagg gcatcatggt cctcactccg gatgctccga
     7621 ccggcatgtc gtttctcaag tatctccatc tggacagtga gcggcttgaa ctcgaattga
     7681 cgccaaaccg tcccgacgcg atgagccatc ggggcgtggc gagggatctt tccgcgcttt
     7741 tcggtcgccc gacaaccaac cccattacct cgccggaaga aaacggccgc ccggtggaag
     7801 agattacctc ggttctgatt gatgaccctg aagggtgtcc ccggtacgtg gcgcgtgttg
     7861 tcgaaggggt aaggatcgca ccctccccgg aatggctggc tggccgtttg cggcaggttg
     7921 gcgtccggcc gatcaacaac gtggtggatg ttaccaacta cgtgctcatg gcgattggcc
     7981 acccgttgca tgcgttcgac cttcaccggc tggcgggacg ccggatcgtt gttcgggctg
     8041 cggaacgcga cgaaagattc acgacgcttg atggaaccga gcgaatcatt cccgaaggaa
     8101 gcgtgttcat ctgcgacggg gagagacccg tggccctggg cggaatcatg ggggggctga
     8161 attcggagat cactgacgac accacggatg tgctgctt
//
LOCUS       DAOTNG010000127         8183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7852_length_8183_cov_5.307825, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000127 DAOTNG010000000
VERSION     DAOTNG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_8183_cov_5.307825"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1450)
                     /locus_tag="P5179_07485"
     CDS             complement(158..1450)
                     /locus_tag="P5179_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95095.1"
                     /translation="MQVRAIIVGAGHRGLGAASYAKHRPEEFAITGVVDPSPLRRERA
                     ASLFDIPAEHCFSTVEELVAYPKCADVAINATMDSLHVPTSLPLLEAGYDILLEKPIS
                     TNIEDLLRLYEAARRLRRTVMICHVLRYAPFYTAIRTRVAAGEIGDIMSVQTVEHVSY
                     HHMAVGFVRGKWRRKDTCGSSMLMAKCCHDLDLIAWMKSGVAPVSVASHGSLMYFRRD
                     KMPEGAGTRCLVDCPIEEGCMYSARKHYIDHPDRWRFYVWDSIEHIAEPTIEDKIESL
                     RTTNPYGLCVWRCDNDVVDHQTVSVEFADGSTAVHSMVGGTARPVRSIHLVGTQGEIQ
                     GVLEDNRFVIRHIDPRPAHEYSEETVDLNVATPELERGGHGGGDKLLVEDFVRFIRGE
                     PRSIAATTLDDSINGHLIGFCADIAREEKRVVDIPRIA"
     gene            complement(1456..2427)
                     /locus_tag="P5179_07490"
     CDS             complement(1456..2427)
                     /locus_tag="P5179_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS95096.1"
                     /translation="MEFGSVPGVEKPVSRIVLGTMIVTTEEKDRSFRLLDDALVLGCT
                     TLDTAHVYAGGNSERAIGQWMAERGNRENVVILTKGAHPNADRRRVTPFDIASDLADS
                     LARLRTEYVDIYLLHRDDPAQPVGPIVEALNEHRAAGRIHAFGGSNWTHHRIAEANEY
                     AEAHGLVPFVASSPNFSLAAQVEDPWGSGSVTLSGPQHADARAWYAGNQIPVFAWSSL
                     ARGFFSGRFTRKTYDAFKDSLDGACRRAYCHEENLERLDRVETLAREKQLTIPQIALA
                     YVMSQPMNVFACVGAADASELAACVEASTVRLTAAEIAWLDLRSESR"
     gene            complement(2442..3443)
                     /locus_tag="P5179_07495"
     CDS             complement(2442..3443)
                     /locus_tag="P5179_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95097.1"
                     /translation="MKKKTDDKPVRWGILATGNIAKQFARGLQVIPDARLVAVGSRTR
                     KGAEAFAKEFNVPNVHASYEALASDPDVDAVYVATPHPFHRENTILCLNAGKAVLCEK
                     PFAINAREAREMIACARQNNVFLMEAMWTRYLPLYAKVRELLSNGVIGDVRMVKADFC
                     FRADLKPEGRLFNLALGGGGLLDIGVYTISFANMICGPKPERMASLAHIGTTGVDEQA
                     GLLLSYPGDKLAVLTCAVRTTTPHEAAIFGTEGMIRIHHPFWHGSEMTLSVNGKPDVQ
                     MSHPFVGNGYNCEAVEVMRCIRSGRKESALMPLDESLAIMETMDAFRLEWGLRYPTE"
     assembly_gap    3461..3560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3561..4606)
                     /locus_tag="P5179_07500"
     CDS             complement(<3561..4606)
                     /locus_tag="P5179_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95098.1"
                     /translation="MTELRAVIIGCGKSTRTAGAKEHGISHCHVQGYQATEGCKVTAC
                     ADIVEEYAASYAALYGLPKYYLDYREMIAAEKPDIVSVCTWPHLHAEMVIHCAKAGVR
                     AIHCEKPMAPTFGEARRMKETCDELGVQLTFNHQRRFEPQYRTVRELANRGVVGKVLR
                     IEAACGDMFDWGTHWFDMINFYNNDVPAEWIIGQVDSRTENKVFGVPIENQGIASFRY
                     ANGVYGFFVSGYGATIWAEHRILGTDGIIEAHMPVVKVRGRGDTEMRTITFPEQKNIG
                     AWSAITFGIWDLVHGLRTGREPELSARKALAGTQLIFGAYESSRRRARVEFPLDIDDN
                     PLHSMMASGEIGPGR"
     gene            complement(4673..5698)
                     /locus_tag="P5179_07505"
     CDS             complement(4673..5698)
                     /locus_tag="P5179_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95099.1"
                     /translation="MPQYSVAIIGTGKAHRSDGATGYGTAHWHAQGYLATPGCTLIAC
                     ADIVPENARAFAEMYNIPHVYVDYREMLSKEQPDIVSVCTWPQLHAQMVVDCANAGAK
                     AVHCEKPMAPTFGEARLMKDTCEKTGTQLTFNHQRRFEERFIFARDMIKAGAVGQLRR
                     LEGSWGNMLDVGTHWIDLLNFYNDDTPADWVTGQVYRAGDQKAFGVDVESQALCYFHY
                     ANDVYGLLLMGQGHSIGAEHRILGSEGIIEVDWPRVRIRTKGDASWQVFDKGATEGSV
                     TRGIQDVVASLSSGKESALSAKKAFAATEVIFATYESSRRRGRVELPLTIDDNPLHSM
                     IASGQIG"
     gene            complement(5750..6871)
                     /locus_tag="P5179_07510"
     CDS             complement(5750..6871)
                     /locus_tag="P5179_07510"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRS95100.1"
                     /translation="MECTRAAVVDYRALCEPPDFSGDAVAEQTRFPIAIEARVLQHLL
                     PREGVSPAWASDFGRILSDSEAIPVTTVDAVLVPDDTLTEDLRVSLEELDGIAQRWGT
                     VVAHLITAVPKKTLSSIVHQYLAAEDEQVLRFGQQVEKVLALPEDAGSLERCLAVIDH
                     LGAWVDRPAMCDIADVAYRLRMRIDEDRALLRQETERQIAEAPAKALDEFRAQTAVGF
                     CLPEQRMAFRGDTLFVFDGEYPAAEMYVLISEYMAELEKRFTAAAIDRDLFRQAAEAE
                     QPASIPDRVRSIVWRRDNGKCAMCGGTTDLEFDHIIPKSQGGASTVANVQVLCVRCNE
                     RKGAQVAWLPFPRAHRTSEDTLDLFRTPLPPRMERDSTC"
     gene            7103..7597
                     /locus_tag="P5179_07515"
     CDS             7103..7597
                     /locus_tag="P5179_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRS95101.1"
                     /translation="MNTQALVTEFVAYLRSRGMRMTPERRHILQHIFSAHTHFEVDDL
                     HVRLRDAGHRISKATVYRTVALLANCGILRSSIAPSGSASEYYELVYGLETPHEHLQC
                     QQCGRVFEVADPVLLGHLRRIAVTMGYELVDHSVKIVGRCVELENKGVCSKSGLQRPP
                     DKPQ"
     gene            complement(7591..>8183)
                     /locus_tag="P5179_07520"
     CDS             complement(7591..>8183)
                     /locus_tag="P5179_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS95102.1"
                     /translation="GIYVEPFAISRILSRDGIQLVERQISGQQHEALNAGTAFIMTKL
                     LENVVVRGTGAGARSQFGLLRPAGGKTGTTNRNSDAWFVGFTPQICCGVWVGFDQRVN
                     MGRGNTGAGAALPIWAQFMKEAHDVLNLPAQDFPKPPEIEAVEICDESYLAATPNCPS
                     TYTEYFRSAEIPEPCTIHRWGRSSPASRREALRRNY"
BASE COUNT         1592 a         2543 c         2209 g         1739 t
ORIGIN      
        1 ttcgcgcccg gctcaacgac cgacacgttc gagtatccgt tcagcttcga gcccggctag
       61 tctgacgcac cagaacagaa aaacgcgtcc gcgggaagaa acacctgcgg acgcgtttca
      121 ttttgcaacc tgcccttttc cccgaccgcc acgcagctca cgcaatgcgc gggatatcca
      181 caacgcgctt ctcctcacgc gcaatgtccg cacagaatcc gatcagatgg ccgttaattg
      241 agtcgtcgag cgtcgtggcg gctatggagc gcggttcgcc gcgaatgaaa cgaacgaaat
      301 cttcaacaag cagtttgtca ccgcccccgt gccctccgcg ttccagttcc ggggtggcca
      361 cattcagatc caccgtttcc tctgagtatt cgtgcgccgg gcggggatcg atgtgccgaa
      421 tgacgaagcg gttgtcttcc agaactccct ggatttctcc ctgcgttccc acaaggtgga
      481 ttgagcgaac aggccttgcc gtgccgccca ccatgctgtg aacggcagtc gatccgtctg
      541 cgaactccac gctcacggtc tgatggtcca ccacatcgtt gtcacagcgc cacacgcaga
      601 gcccgtaagg gttcgtggtt cggagagact caatcttgtc ttcgatggtt ggttctgcga
      661 tgtgctcgat gctgtcccag acgtagaaac gccaccgatc tgggtgatca atgtaatgct
      721 tgcgcgcaga atacatgcac ccttcttcta tggggcaatc aacgaggcac ctcgttccag
      781 ctccctccgg cattttgtcg cgacggaaat acatcagtga accatgactt gccacggaaa
      841 ccggcgccac cccgctcttc atccacgcga taagatcgag atcatggcaa catttcgcca
      901 taagcatgga ggacccgcat gtgtccttcc tgcgccattt gccgcgcaca aagcccacag
      961 ccatgtgatg gtaacttacg tgttcaacgg tttgcacgct catgatgtcg ccgatttcgc
     1021 ccgccgccac gcgtgtgcgg atggcggtgt agaacggggc gtacctcaga acgtgacaga
     1081 tcatcacggt tcgtctcagt cggcgggccg cctcgtacag ccggagaagg tcttcaatat
     1141 tcgtagatat cggcttttcc agcagaatgt cgtaccctgc ttccagaagt ggcagcgagg
     1201 tgggtacatg cagcgaatcc atggtggcat taatcgcaac atccgcacat ttgggatatg
     1261 cgaccagttc ctccaccgtt gagaagcagt gctccgccgg tatgtcgaac aacgacgcgg
     1321 ctcgctctcg acgcaacggc gaggggtcca ctacgccggt gattgcgaac tcctccggac
     1381 ggtgtttggc gtacgaagcg gctcccagac ctctgtggcc ggctccgaca atgatggctc
     1441 gcacctgcat gttccctacc tgctttcgga gcgaaggtca agccaggcga tttccgcggc
     1501 ggtcaaacgg actgtgctcg cttccacaca cgctgccagt tcgctcgcgt ccgcagcgcc
     1561 gacacaggcg aacacgttca tcggttggct catgacgtat gccagggcga tctgcgggat
     1621 cgtgagttgc ttctcccgcg caagcgtttc tacccggtca aggcgctcaa gattctcctc
     1681 gtggcagtac gccctgcggc acgcaccgtc cagcgagtct ttgaacgcgt catacgtctt
     1741 ccgggtgaac ctcccggaga aaaagccacg ggcgaggctg gaccatgcga acacgggaat
     1801 ctgattcccg gcgtaccagg cacgcgcatc cgcgtgttgc ggaccactca gggtaaccga
     1861 tccgcttccc cacggatcct cgacctgtgc cgcaagactg aaattcggac tgctcgcgac
     1921 aaacggaaca aggccgtgcg cttccgcata ctcattcgct tcggcaatgc ggtggtgagt
     1981 ccagttggat ccgccaaagg cgtgaattct gcctgccgcc ctgtgctcat tcagcgcctc
     2041 tacgatcggc ccgaccggct gcgcggggtc atcccggtgg agaaggtaga tatccacgta
     2101 ctccgttcgc agtcgtgcga gcgaatcggc aagatcggac gcgatgtcga aaggtgttac
     2161 ccttcggcgg tccgcattcg ggtgggcgcc ctttgtaagg atgacgacgt tctcccgatt
     2221 accccgctca gccatccact ggcctatcgc ccgttcactg ttaccgcccg catatacgtg
     2281 cgccgtgtcc agcgttgtgc agccgagcac gagggcgtca tcaagcaggc ggaaactccg
     2341 gtccttttcc tctgtcgtaa caatcatcgt accgaggaca atacggctta cgggcttctc
     2401 aacgccggga accgacccga attccatgga gtgaatctcc atcattctgt ggggtatctc
     2461 aggccccatt ccaaacggaa cgcatccatc gtctccatga tcgcaaggga ttcgtccagc
     2521 ggcatcaggg cactctcttt tcttcccgac cggatacacc gcatgacctc gaccgcttcg
     2581 cagttgtacc cgttcccgac aaatggatgg gacatctgca cgtcgggttt gccattcacg
     2641 gagagcgtca tctcgctccc gtgccagaac gggtgatgga tccgaatcat gccctcagtt
     2701 ccgaaaatgg cggcctcgtg tggtgtggtg gtacgaaccg cgcatgtcaa taccgccagt
     2761 ttgtcgccgg ggtaactcaa cagcagcccc gcctgttcgt caacacccgt cgtgccgatg
     2821 tgcgcgagag atgccatccg ttcgggcttc ggaccgcaga tcatgttcgc gaacgaaatc
     2881 gtgtatacgc caatatccag aagcccgccg ccaccgagtg ccagattgaa caggcgacct
     2941 tcgggcttca gatccgcgcg gaaacagaaa tcggctttca ccatgcgtac gtcaccgatc
     3001 acgccgttcg aaagaagctc cctgaccttc gcgtacagcg gcagatagcg cgtccacatg
     3061 gcttccatca ggaacacgtt gttctgtcgt gcgcacgcga tcatttcccg tgcctcgcgc
     3121 gcattgatcg caaagggctt ttcacaaaga acagccttcc ccgcgttcag gcagagaatc
     3181 gtgttctccc tgtggaaagg gtgcggcgtt gcgacataaa ccgcatccac gtccgggtcg
     3241 cttgccaacg cctcgtaaga tgcatggaca ttggggacgt tgaactcctt ggcgaacgcc
     3301 tccgcccctt ttctcgttct ggaaccaacc gcgacaagcc gcgcatcggg gattacctgc
     3361 aggccgcgcg caaattgctt cgcgatgtta ccggttgcca gtatgcccca gcgcaccggt
     3421 ttgtcgtccg tctttttctt catgtgttga cctcagaatc nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn cgtcccgggc caatctctcc agaagccatc atgctgtgga
     3601 gggggttatc gtcaatatcg agcgggaatt ctacacgtgc ccggcggcgg ctggactcgt
     3661 acgccccgaa aatgagctgg gttcctgcca gcgcctttcg agccgaaagc tccggctcgc
     3721 gtcccgtccg cagtccgtga accaggtccc atatgccgaa cgtgattgcc gaccacgcgc
     3781 cgatattctt ctgctcgggg aaggtgatcg tgcgcatttc cgtgtcgcct cttccacgga
     3841 ctttcaccac cggcatgtgc gcttcaatga tgccgtctgt tccgagaatg cggtgctccg
     3901 cccagatcgt cgcaccgtaa ccgctcacga aaaagccata cacgccattc gcgtaacgga
     3961 aactcgcgat tccctggttc tcgatcggca cgccgaacac cttgttttca gtccggctgt
     4021 caacctgtcc gataatccac tctgccggaa cgtcgttgtt gtagaagttg atcatgtcaa
     4081 accaatgggt gccccagtcg aacatgtccc cacaggcggc ctctatgcgc agcactttgc
     4141 ccacaactcc ccggttggca agttctctga ccgtgcgata ctgcggttcg aagcgtcgct
     4201 ggtggttgaa cgtgagctgc acgcccagtt cgtcgcatgt ttccttcatt ctgcgtgctt
     4261 caccgaaggt cggtgccatc ggcttttcgc aatgaatcgc ccgcacgccg gcctttgcgc
     4321 agtggatcac catctccgcg tgcaggtgcg gccaggtgca cacgctcacg atatccggtt
     4381 tctccgcggc aatcatctcc cggtagtcga gatagtactt gggtaacccg tagagagcgg
     4441 catagctggc cgcgtattcc tccacgatat ccgcgcatgc ggtaactttg cagccttccg
     4501 tcgcctggta cccctgcacg tggcaatggc tgattccgtg ctctttggcg ccggcagttc
     4561 gcgttgactt gccgcagcca ataatcactg cgcgaagttc tgtcattgtg tctcgtctcc
     4621 gtcactggtg gaactgggta accattcttg tctcatcaca gcccaactgc cgtcacccga
     4681 tctggccgct ggcgatcatc gaatgcagcg ggttgtcgtc aatcgtgagc ggcaattcca
     4741 cacggccccg ccgcctgctg gactcgtacg tcgcgaatat tacttcagtc gccgcaaagg
     4801 ccttcttcgc gctcagcgcg gattccttcc ccgagctcag actcgccacg acatcctgaa
     4861 tgccccgtgt taccgacccc tcggtagcgc ctttgtcgaa aacctgccac gatgcatcgc
     4921 cttttgttcg aattctcaca cgcggccaat ccacttcgat gatgccttca cttcccagaa
     4981 tgcggtgctc tgcgccaatg gaatgcccct gccccatcag caggagcccg tacacatcat
     5041 tcgcataatg gaaatagcag agcgcctggc tctccacatc cacaccgaac gctttctggt
     5101 ctccggcgcg gtatacctgg ccagtaaccc agtccgccgg agtatcgtca ttgtaaaagt
     5161 tcagcaggtc gatccagtgt gtgccgacgt ccagcatgtt gccccaggat ccttcgagcc
     5221 gtcgcaattg tccgacggca cccgccttga tcatatcgcg cgcgaagatg aaccgctcct
     5281 cgaaacggcg ttgatgattg aacgtaagct gtgttcccgt tttctcgcag gtatccttca
     5341 tcaagcgcgc ttcgccgaag gttggagcca taggcttctc acagtgaacc gctttcgcgc
     5401 cggcgttggc gcaatcaact accatctggg cgtgaagctg gggccatgtg catacgctca
     5461 ctatgtccgg ttgttctttg ctgagcatct cacggtagtc cacgtacaca tgcgggatgt
     5521 tgtacatctc cgcgaatgcg cgtgcatttt ccggaacgat gtctgcgcat gcaatcagcg
     5581 tgcatccggg tgtcgcaagg taaccctggg cgtgccagtg cgcggttccg tacccggtgg
     5641 ccccatcaga tcggtgtgct tttcctgtgc ctatgatggc tacggagtat tgtggcatag
     5701 tgtggtcctt tgtgctcgtg tggtgcccga tggacagccc tggagggaat cagcatgtgg
     5761 aatcgcgttc cattcgtggg ggcaaaggcg ttctgaaaag atccagtgtg tcctcggagg
     5821 tccggtgcgc gcgaggaaaa ggaagccagg ccacctgtgc gcccttcctc tcattgcatc
     5881 ggacgcacag aacctgaacg tttgcgaccg ttgatgcccc gccctgggac ttcgggatga
     5941 tgtggtcgaa ttcgagatcg gtggtccctc cgcacatcgc gcatttgccg ttatcccggc
     6001 gccacacgat ggaccgcaca cgatccggaa tggaggcggg ttgctcggct tctgccgcct
     6061 gcctgaaaag gtcccggtca atcgctgccg cggtgaaccg tttctcgagc tcagccatgt
     6121 attctgagat caacacgtac atctccgccg ccgggtattc cccgtcgaaa acgaaaagcg
     6181 tgtctccccg gaacgccatg cgctgttccg gcaggcaaaa gcccacggcg gtctgagccc
     6241 ggaactcgtc gagcgccttc gcgggtgcct ctgcgatctg ccgttcggtt tcctggcgca
     6301 aaagagcgcg atcttcgtca atccgcatgc gaagacgata ggccacatcg gcgatatcac
     6361 acatcgccgg acgatccacc cacgcgccca ggtgatcaat cacggcaaga caacgttcga
     6421 gactgccagc gtcctccgga agggcaagca ctttttccac ttgctggcca aagcgcagga
     6481 cttgttcgtc ttcggctgcc aaatactgat gcacgatcga cgagagtgtc ttcttgggca
     6541 ccgcggttat caggtgcgca acaacagtgc cccaccgctg cgcaatgccg tcaagttcct
     6601 cgagactcac gcgcagatct tcggtaagcg tgtcatcagg cacaagaacg gcgtcaacgg
     6661 tggttaccgg aattgcctct gaatctgaca aaatccgccc aaaatcagag gcccaggcag
     6721 gtgacacgcc ttcacgtggg agaagatgct gcaacactct cgcttcaatt gcaataggga
     6781 atcgcgtctg ttctgccacg gcgtcgccgg aaaaatccgg aggctcacac aacgcccggt
     6841 aatccaccac ggcggcacga gtgcattcca tcctctcgcc cttcccggaa acgtgccagt
     6901 ttcatagcgg cgcaaagaac gcagccggcg tgcgatgccc cccatcaatt agtacttttt
     6961 ttccggcaag ggggcgaggg ggtgcgtctc tgcgccgcgc cgaatctcgc acgaggaaaa
     7021 acgctccgcc ggacgctgtt gaccggtcgc cgatgtgcgg tatcttcaat ttgacgaaga
     7081 acctgagacg gatggacgac caatgaatac gcaggcgctt gtcaccgagt tcgttgccta
     7141 tttgcgctcg cggggaatga gaatgacgcc cgagcggcgt catattctcc agcacatctt
     7201 ctccgcccac acgcacttcg aagtggatga cctgcatgtt cgcctgcggg atgcagggca
     7261 caggatatcc aaagccaccg tgtaccgcac cgtcgccttg cttgctaatt gcggaatact
     7321 gcgctcctcc atcgccccca gcggatctgc ctcggaatac tacgaactcg tctacgggct
     7381 ggaaaccccc cacgagcatc tgcaatgcca gcagtgcggg cgcgtgttcg aggttgccga
     7441 tccggttctt ctcgggcacc tccgacggat tgccgtcacc atgggatacg aactcgtgga
     7501 tcactccgtc aaaatagtcg gcaggtgcgt ggagctcgag aacaaaggtg tgtgctccaa
     7561 atccggcctt caacgccctc cggacaagcc tcaatagtta cgccgcaatg cttcacggcg
     7621 tgaagccggt gagcttctgc cccagcgatg aattgtacac ggctcaggga tttctgcaga
     7681 acggaagtat tccgtgtagg tgctcgggca attcggagtc gcggcaagat agctctcgtc
     7741 acagatctct actgcctcaa tttctggagg ttttggaaag tcctgtgccg gcagattgag
     7801 cacatcgtgg gcctccttca tgaactgcgc ccagatcggt agtgctgctc cggcacccgt
     7861 gtttccccgt cccatgttca ctcgttgatc gaaccctacc cacacgccac agcagatctg
     7921 aggagtgaag ccgacgaacc acgcgtcgct gttgcggttg gtggtgccgg tcttgccgcc
     7981 ggcaggccga agcagcccga attgagaacg cgcaccggca cccgtacctc ggacaaccac
     8041 attctcgagc agttttgtca tgatgaacgc ggttcccgca ttcagcgctt cgtgctgctg
     8101 gcccgaaatc tgccgctcca cgagctgaat cccgtccctc gaaagaatgc gcgaaatggc
     8161 gaagggctcc acgtagatac cct
//
LOCUS       DAOTNG010000128         8100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7949_length_8100_cov_4.935736, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000128 DAOTNG010000000
VERSION     DAOTNG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_8100_cov_4.935736"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1194)
                     /locus_tag="P5179_07525"
     CDS             complement(310..1194)
                     /locus_tag="P5179_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95103.1"
                     /translation="MTPVLFSVSYAGLWGQDTLDVESFIERAAKLFYPAVELMGKRPH
                     LSVLDASPERIRRIRERAAATNVEIVTIAAYTGFTAGPAPEVPFGEMQVLYVRELARI
                     ARLLDAHVIRVFTGYSTDDHSYVTDWKRCVAGIREAATVAADQGIVLGVQNHHDTAIS
                     TDAYVEFLDEVNHPNCKAMYDPWVPALLGEDLYACAKRLAPRMVQTTLADYVRIPRWA
                     YEPGLVNYRKLPDMVRAVPLGEGFIDLNAFFRGLKEGGFDGYVAYEMCSPLRGGGGYE
                     NLDHAAGTSLRRITALIG"
     gene            1383..2567
                     /locus_tag="P5179_07530"
     CDS             1383..2567
                     /locus_tag="P5179_07530"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS95104.1"
                     /translation="MYRMGQEEVDEVAKVILAKQLFRVGDPKAGHLQEVDRFEKEWAE
                     KIGTDYALMVSGGGTAALICGLAGMGIGPGDEVIVPAYTWLATASAVLSVGGIPVLAE
                     IDETMALDPDDFERKIGPHTKAVIPVHMLGRPADMERIVAIARRHSVKVLEDSCQNNG
                     GTYRGRRTGTWGDAGAFSLNYYKIISCGEGGILVTNDEKIAERAVIFHDSGTAFRPKA
                     GELTEPIFIAQQYRASEVMGAIARIQLQRMDGIIADLQRVRRTIEAALAGEPGLKFAP
                     SNDPDGDSGVVVAFQFDTEAKARAFQQSPGVGGYVGIDHGKHVYTEWTPIREKRFMHH
                     PAMNPFNFPQNKGLRADYSDTACPRSLDLLRRTVFCSLDPDWTEQKVAEKIAAFKMAA
                     KG"
     gene            2635..3396
                     /locus_tag="P5179_07535"
     CDS             2635..3396
                     /locus_tag="P5179_07535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95105.1"
                     /translation="MTIIDANASFGAVYTPTLAPCRTPEELIAALDRAGVDRALVAAD
                     AIRGGCPSEMNVRAALELAPHARLMPVWAILPLETGEFGTRTEFLDSMKRYGVRALAA
                     YPSKHRYLLDRVGCGELFDAMVECRIPLLLPLEESSGGVGGWALAEKLLSETPGLRLI
                     VLGTGPWGDDRLFRPLLRSFPTLTVEVSRYELDGGIAALCGKYGAERLVYGSGYPHWA
                     MGGALLTALHADIPEADKGLILGGNIARMLDEVTI"
     gene            3393..4214
                     /locus_tag="P5179_07540"
     CDS             3393..4214
                     /locus_tag="P5179_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95106.1"
                     /translation="MNHATPLMEEFRRLGKSVSCPIIDMHGHFGPFNGIFFPNVTAEQ
                     MVGSMDRAGVRLIVCSSHEALLVNSETGNARMADVVRRFPDRFRAYWGVNPNEPDVLL
                     RQLDGLAERKEFVGIKFLSDYHRYPITGDAYVPALKYAQEHRMPVLMHTWGGSKFDGP
                     ELVGEVADRYPDLVILMGHSGYGEWDVSISVARKHPNVYLELTAAYAVNGVVEKLVNK
                     AGSEKVLFGTDLPWFDPHYAAGCILYAHISDNDRHNIFHRNAERILAECGNPVRF"
     gene            complement(4376..5824)
                     /locus_tag="P5179_07545"
     CDS             complement(4376..5824)
                     /locus_tag="P5179_07545"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF010322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRS95107.1"
                     /translation="MPRPNVILILCDQMRGDAFRADGNRFIETPNIDYLASRGTRFRH
                     AYTAVPSCIPARSILWSGQRQWHTGVLGQGRGQGPMPNDFAHTIAGEFASAGYRTHLV
                     GKGHFNPHRASMGFQTMELDESGRMPDSEHRQWFRTHAPEGVTPDDHGVAFNSWHARP
                     WHTEEHLHPTAWTMSRSLAFLESHDSTQPFFLNISFARPHSPYVPPRYYFDLYHNRET
                     PPPHVGEWSARNDDPVTAVDPNAWRGKQTPEAIHRARAAYYGEVSFIDTQIGRLMNWC
                     SRYRRAAWENSWIVFVADHGDMQGDHHLWRKNHAYEGSARIPFIVVPPKEFRGAKRAV
                     AYEVVELRDIMPTLFEAAGIPIPPTAEGRSLLPLLDAPASDWRPYIHGEHSQNYSPEQ
                     EMQYVTDGRRKFIWLPRINVEQFFDLESDPGERVNLIDDPSRQSEIATWRGYLVKELA
                     ERDCGWVVDGRLHCPPEPLVSPWKDVRWIGTR"
     gene            complement(5862..6428)
                     /locus_tag="P5179_07550"
     CDS             complement(5862..6428)
                     /locus_tag="P5179_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HRS95108.1"
                     /translation="MDNLGTKGFIPPHGNYRGLLAYQKATVVYDLTFRFCDRLLKRGD
                     RTVDQMVQAARSGKQNIVEGSTASGTSKEMEIKLTSVARASLEELLEDYRDFLRVRNL
                     VLWDKDSKEAKYIRKLGSEPNTGYDAYREFAETRSAEVVANIAICVIHQANYLLDQRL
                     RRLEQDFVQDGGIRERMARTRLQRRAAE"
     gene            complement(6534..7985)
                     /locus_tag="P5179_07555"
     CDS             complement(6534..7985)
                     /locus_tag="P5179_07555"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF010322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRS95109.1"
                     /translation="MPRPNIILIMCDQMRGDAFGADGNSSIETPNLDFLASLGTRFCH
                     AYSAVPSCLPARAILWTGQNQWHTGVLGMGRGQGQIPHDFPHTLAGELANAGYRTHLV
                     GKGHFHPQRAPMGFHDMELDESGRMPTSEHRQWFRTHAPEGVTPDDHGVDWNSWHSRP
                     WHTDEYLHPTAWTMSRALNFLDTFDYSQPFFLNISFARPHSPYVPPRFYWDFYHSRGT
                     PPPSVGEWAIMHDDPHTARDPNAWRGKITPDRIHRARSGYYGEISFIDTQIGRLIKRL
                     NRGNDHASFANTWIVFTSDHGDMQGDHHLWRKTYAYEGSARIPFVIVPPRALGRPSRQ
                     VADEVVELRDIMPTLLEGAGVALPGTVDGQSLVPLLDAPAAEWRPYIHGEHCTCYSEE
                     QEMQYVTDGRRKFIWLPRINVEQFFDLECDPGECHDLIHDPLRQTEIAAWRGYLVNEL
                     AKRDCGWVVDGKLHCPPDPLVSPWKDVRWVGQS"
BASE COUNT         1520 a         2380 c         2599 g         1601 t
ORIGIN      
        1 ggtagagcca tccgtgttcg tcgccacgtg cgcggaacgg ggtttgaggt ccgcgccatc
       61 cacacgcaac gcaggggaca gcccgcgcac ggcaaatccg ggccaggatg ggcaggagat
      121 gccgacggtg cccgtcgggg taacctcgag ttgggtcgtc tcgtgtttca ttgtgggtcc
      181 tgttcttccg agagattttt cactccgtct ggaatgacga gaagcgcaac ggccacgaga
      241 agaagggccg ccgggcgatc aaccaggatg tttgtctcac tcgcacccgg tgcaagttcc
      301 cttggccttc tatccgatca gcgcggtgat gcgccgcagg cttgtgccgg ctgcgtggtc
      361 gaggttttcg tacccgccgc caccacgaag gggcgagcac atttcgtacg caacatagcc
      421 gtcgaacccg ccctctttca gcccgcggaa gaacgcattc aggtcaatga acccctcccc
      481 cagcggcacc gcgcgcacca tatccggcag ctttctgtaa ttgaccagac ccggctcgta
      541 cgcccaccga gggatccgca cgtagtcggc gagtgtcgtt tgcaccatgc ggggagccaa
      601 tcgttttgcg caggcgtaca ggtcctctcc cagcagggcc ggcacccagg ggtcgtacat
      661 cgccttgcag ttcgggtggt tcacctcgtc gagaaactcg acgtacgcat ccgtagaaat
      721 tgccgtgtca tggtgattct gcacgcccag cacaataccc tgatcggccg ccaccgttgc
      781 agcttcacgt atgccggcca cgcatctctt ccagtcggtg acgtacgagt ggtcatcggt
      841 ggagtacccc gtgaatacgc ggataacgtg cgcatcgagg aggcgggcga tacgcgcaag
      901 ctcccgcacg tacagcacct gcatctcacc gaagggtacc tccggcgcgg gaccggctgt
      961 gaaaccggtg tatgccgcga tggtaacgat ctccacattg gtggcggcgg ctcgttcccg
     1021 gatacgcctg attcgttccg ggctcgcgtc gaggacggaa agatggggcc tcttgcccat
     1081 caattcgaca gccgggtaga aaagcttcgc cgctcgttcg atgaacgact ctacgtccag
     1141 cgtgtcctga ccccagagcc ccgcgtagct tacggagaag agaaccggtg tcatacgtat
     1201 ccctcccgaa tggttttctg tccggcgttc cgccattaac ggtagtctgt ctgccgcgca
     1261 gggccaagag cgggatggct gcccactgct cagcttttcg ggagtcatct acaggtcgca
     1321 gatttcgtta acttttatat gtgttgcatg ctaccacccc cgtttgagaa ggaatccgcg
     1381 ctatgtaccg catgggtcaa gaggaagtag acgaagtcgc gaaggtcatt ctggcgaagc
     1441 agttgttccg cgtgggcgat ccgaaagcgg ggcacctgca agaggtagat cgtttcgaaa
     1501 aggagtgggc ggagaagatc ggaacggatt acgcgctcat ggtaagcgga ggcggcactg
     1561 ccgcgcttat ctgcggcctt gcggggatgg gtatcggccc tggcgacgag gtgattgtcc
     1621 ccgcatatac ctggctggca actgcgtcgg cggtgttgtc cgtcggcggg atccctgtgc
     1681 ttgcagaaat tgacgagacc atggcgctcg acccggatga tttcgagcgc aagatcgggc
     1741 cgcacacgaa ggcagtcatc ccggttcata tgctcgggcg ccccgcagat atggagcgca
     1801 tagtcgccat tgcccgcagg cacagcgtga aagtgctgga agactcatgc cagaataacg
     1861 ggggcacgta cagaggacgg cggaccggta cgtgggggga cgcgggagca ttcagcctca
     1921 attactacaa aatcatctcc tgcggcgaag gtggaatact ggtaacgaac gacgagaaga
     1981 tcgctgaacg cgccgtaatc ttccacgact ccggcacggc gttccgcccc aaggcaggcg
     2041 aactcactga gccaatcttc attgctcagc agtaccgcgc atcggaggtc atgggcgcca
     2101 ttgcgcgaat tcagctccag cgtatggacg gcatcatcgc cgacctgcaa cgtgttcgac
     2161 gcaccatcga agccgctctg gcaggggagc cgggccttaa gttcgccccg agtaacgacc
     2221 ccgacggcga cagcggcgtg gtggtggcgt tccagttcga caccgaggcc aaagcccgtg
     2281 cgtttcaaca atcaccaggc gtgggcggtt acgtcggcat agaccacggg aaacacgtgt
     2341 acacggagtg gacacccata cgcgagaagc gcttcatgca tcaccctgcc atgaacccgt
     2401 tcaacttccc tcagaacaag ggcctgcgcg cagattacag cgacaccgcc tgcccgcgct
     2461 cgctggactt gctgcgccgc acggtgttct gctcgcttga cccggattgg acggagcaga
     2521 aagtcgcgga aaagatcgcc gcgttcaaga tggctgcgaa aggctgaaga gaagtcaacg
     2581 cgcaccattt ttccccggag ggcgcgttcc cgcccaccgg caggaggaac cttcatgacc
     2641 atcattgacg cgaacgcgtc gttcggcgca gtgtacacac cgacgcttgc cccttgcagg
     2701 acgccggagg agttgatcgc cgcgcttgac cgcgccggtg tggacagggc gctcgtggcg
     2761 gcggatgcga ttcgcggtgg atgcccgtcg gaaatgaacg tccgtgcggc cctggaactc
     2821 gctcctcacg cgcggttgat gcctgtgtgg gccatcctgc cgcttgaaac gggagagttc
     2881 ggcacacgga ccgaattcct cgattccatg aagcggtacg gcgtccgtgc actggcggca
     2941 tatccctcga aacaccggta tctgctcgac cgggtgggct gcggggagct tttcgacgcc
     3001 atggtggagt gccgcattcc gctgttgctg ccgctggagg agagttctgg cggcgtgggc
     3061 ggctgggcgc tcgcagaaaa actcctgagt gagacacccg ggctgcggct tatcgtgctg
     3121 ggcaccgggc cgtgggggga tgaccggctc ttccgcccgt tgctgcgaag ctttcccacg
     3181 ctgaccgttg aggtctctcg ttacgagctt gacggcggca tcgcggcact gtgcggcaaa
     3241 tacggcgcag aacggcttgt gtacggttcg ggataccccc actgggccat gggtggggcg
     3301 ctgttgaccg cactccatgc cgacatccct gaagccgaca agggactcat tctgggcggc
     3361 aacatcgccc gaatgctgga cgaggtgacg atatgaacca tgctacgccg cttatggagg
     3421 aattccgccg gctcggaaaa agcgtgtcgt gcccgattat cgacatgcac ggtcacttcg
     3481 ggccattcaa cggcatcttt ttcccgaacg tgacagccga acagatggtc ggttcgatgg
     3541 acagggcggg tgtgcggctc atcgtctgca gttcgcacga agcgctgctc gtcaacagcg
     3601 agacggggaa cgcccgcatg gccgatgtgg tgcgtcgctt ccccgatcgt tttcgcgcat
     3661 actggggcgt caatccgaac gaaccggatg tcttgctgag gcagcttgat ggactggcgg
     3721 agcgcaagga gtttgtcgga atcaaattcc tctccgacta ccaccgctac ccgatcaccg
     3781 gcgatgcata tgtccccgca ctgaaatacg cgcaggagca ccgcatgccg gtgctgatgc
     3841 atacatgggg cggcagcaag ttcgatgggc ctgaactggt gggcgaggtg gccgaccgct
     3901 acccggacct tgtcattctg atggggcatt ccggttacgg cgagtgggac gtgtcgatca
     3961 gcgtggcgag gaaacaccca aatgtgtacc tcgaactgac cgcggcgtat gcagtgaacg
     4021 gggtagtgga gaagttggtg aacaaggcgg gatcggagaa ggtgcttttc ggcaccgatt
     4081 tgccctggtt cgacccgcat tatgcggcgg gatgtatcct ctacgcccac atcagcgaca
     4141 atgaccggca caacatcttc caccgcaacg cggaacggat tctggcggag tgtgggaatc
     4201 cggtgaggtt ctgacagggc gcacacgcca ggcgccgtgt gcgtcattcc cgcctgccaa
     4261 gatggggctg cacgggatta ttcgtcccat gggacgaata tggcgcacaa gcagcgatgt
     4321 ccccaaaacg gcccctctat atgcattgca ccccaacgcg ttcattcgcg gcgatctacc
     4381 gggttccgat ccagcgcacg tccttccacg gagaaacaag tggctcgggc gggcagtgaa
     4441 gtctgccatc gaccacccag ccgcagtcgc gttccgccag ttccttcacg agataccccc
     4501 gccacgtggc aatctcgctc tgccgtgacg gatcatcaat caggttgacc cgttctcccg
     4561 gatcagattc gaggtcgaag aactgctcga cgttgatgcg cgggagccag atgaacttgc
     4621 gacgtccgtc ggtgacgtac tgcatctcct gttcgggcga gtagttctgg ctgtgttccc
     4681 cgtggatgta cggccgccag tcggacgcag gggcgtcaag gagcgggagc aggctccggc
     4741 cttccgcggt ggggggaatc gggatgccgg cggcctcgaa cagcgtcggc atgatgtcgc
     4801 gcagttccac cacttcgtac gcgaccgccc gtttcgcacc gcggaactcc ttcggaggca
     4861 ccacaatgaa cggtatccgt gcgcttccct cgtaggcgtg gtttttgcgc cagagatggt
     4921 ggtcgccctg catatcgccg tggtccgcga cgaaaacaat ccacgagttc tcccatgcgg
     4981 cccggcggta ccgggaacac cagttcatga gtctcccgat ctgggtgtcg atgaacgaaa
     5041 cttccccgta atacgcggca cgggcccggt gaatggcctc cggcgtctgt ttgccgcgcc
     5101 acgcgtttgg atccacggcg gtgactggat cgtcgttacg ggcggaccat tcacctacgt
     5161 gagggggcgg cgtctcgcga ttatggtaga ggtcgaagta gtaccggggc ggaacatagg
     5221 gtgaatgggg tcgggcaaac gagatgttga ggaaaaaggg ctgtgtggag tcgtgcgatt
     5281 cgagaaacgc gagcgaccgg ctcatggtcc acgcggtcgg gtgaaggtgc tcttcggtat
     5341 gccacggcct cgcgtgccat gagttgaagg cgaccccgtg gtcgtccggt gtcacgcctt
     5401 ccggtgcgtg cgtgcggaac cattgcctgt gttcactgtc gggcatccgg cctgattcgt
     5461 ccagttccat cgtctggaag cccatggagg cgcggtgtgg gttgaagtgc cctttcccga
     5521 cgaggtgggt acggtagccg gcgctggcaa attcgccggc gatcgtgtgg gcgaaatcgt
     5581 tcggcatcgg gccctgcccg cgtccctggc ccagcacgcc ggtatgccac tggcgttgcc
     5641 cgctccagag gatcgaacgc gccggaatgc acgacggcac cgcggtgtag gcgtggcgga
     5701 accgggttcc ccgcgaggcg aggtaatcaa tgttcggtgt ttcgatgaaa cgattgccgt
     5761 ccgcgcggaa ggcgtcgccg cgcatctggt cgcagagaat caagatcacg ttggggcggg
     5821 gcatggggca gctcctctag tttcgggcgg cgttggggtg ttcactcagc agcgcgccgt
     5881 tgaagacgcg tcctcgccat gcgttcgcgt ataccgccat cctgcacgaa atcctgttcc
     5941 aaccgacgca aacgctgatc gagaaggtag ttggcctggt ggatcacgca tattgcgatg
     6001 ttggccacca cttccgcgga ccgggtctca gcgaactccc tgtacgcgtc ataccccgtg
     6061 ttcggttcgc tgcccagttt ccggatgtat tttgcctcct ttgagtcctt gtcccacagg
     6121 accaggttac gaactcgcag gaaatcacgg tagtcttcca gcaattcctc caggctggca
     6181 cgtgccacgc ttgtcagctt gatctccatt tcctttgacg ttcctgaagc tgtgctcccc
     6241 tccacaatgt tctgttttcc cgaacgtgcg gcctgaacca tctggtcgac cgtgcggtcg
     6301 cctcttttga gaagcctatc gcagaaacga aaagtgagat catacacgac tgtggccttc
     6361 tggtatgcga gcaggccacg gtagtttccg tggggaggga taaaaccctt ggtgcctagg
     6421 ttgtccatgg tagtaatacg tcctataggc caaatacgga gtatatgaag cgatgcacca
     6481 gtaatttcca actgagcagc acggccaaac aggccgtatt ctgcttggcg tcatcagctt
     6541 tggcccaccc agcgcacgtc cttccacggg gaaacgagcg ggtcgggcgg acaatggagt
     6601 ttgccgtcca ccacccagcc gcagtcgcgt ttcgccagtt cgttcacgag atatccgcgc
     6661 caggcagcga tttcagtctg ccgcaacgga tcgtggatga ggtcgtggca ttctccggga
     6721 tcgcattcga gatcgaagaa ctgctcgacg ttgatacgcg gcagccagat gaacttgcgc
     6781 cggccgtcgg tcacatactg catctcctgc tcttccgagt agcacgtgca gtgttcgcca
     6841 tggatgtacg ggcgccattc ggcggcgggc gcatcgagca aaggcacgag gctttgtccg
     6901 tccacggtcc ccggaagtgc tactcccgcg ccttccagaa gcgtaggcat gatgtcgcgg
     6961 agctccacca cttcgtcggc cacctgccgt gaaggcctcc ccagtgctct cggcgggacg
     7021 ataacgaacg gaatgcgggc gctgccttcg taagcgtagg ttttgcgcca gaggtggtgg
     7081 tcgccctgca tgtccccgtg gtccgaggtg aacacgatcc aggtgttggc gaaactggcg
     7141 tggtcgttgc cccggttgag gcgtttgatg agccggccga tctgcgtgtc gatgaacgaa
     7201 atctcaccgt agtagccgct tcgcgcgcgg tggatgcgat cgggggttat ctttccgcgc
     7261 caggcgttgg ggtcacgcgc ggtgtgcggg tcgtcgtgca tgattgccca ttcgcccacg
     7321 gaaggcggcg gggttccgcg gctgtggtag aaatcccagt aaaatcgcgg cggaacgtac
     7381 ggtgaatggg gtcgcgcaaa cgaaatgttc aggaagaaag gttgcgagta gtcgaatgtg
     7441 tcgagaaaat tgagcgcgcg gctcatcgtc cacgcggtgg ggtggaggta ctcgtccgtg
     7501 tgccagggcc ggctgtgcca ggaattccag tccacaccat ggtcgtccgg ggttacgcct
     7561 tccggcgcat gagtgcggaa ccactggcga tgctcgctgg tgggcatgcg accggattcg
     7621 tccagttcca tgtcgtggaa acccatcggg gcgcgctggg gatggaaatg gcctttgccc
     7681 acgaggtgcg tccggtaacc agcgttggcg agttcaccgg caagggtgtg cgggaagtcg
     7741 tgcggtatct gtccctgccc gcgtcccatc ccgagcacgc cggtgtgcca ctggttctgt
     7801 ccggtccaga ggatggcgcg cgcgggcagg cacgacggca ccgccgaata cgcgtgacag
     7861 aaccgcgtgc ccagtgatgc gaggaaatcc agattcgggg tttcgatgga ggagttgccg
     7921 tcggctccaa acgcgtcccc tcgcatctgg tcgcacatga taaggatgat gttggggcgg
     7981 ggcatgggcg cctccttgtc ggcaaggttt cattcgcata gtgctgtaga aggagattac
     8041 tccgggcgcg ttgggcaacg caaggcgggg aacggtgcgt ccgcaacgca agggactctg
//
LOCUS       DAOTNG010000129         8093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7956_length_8093_cov_5.128017, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000129 DAOTNG010000000
VERSION     DAOTNG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_8093_cov_5.128017"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="P5179_07560"
     CDS             <1..1030
                     /locus_tag="P5179_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95110.1"
                     /translation="LASLISVFGTSLILYLGALYVRDRVITVGDLLYFHSSVANLFSP
                     VVVLTGLNATVQWVLVVLRRVFDILDEPITTSDPPNPVPLTSVRGEVEFDHVSLRYPN
                     ATTNALTDINLKVPAGTTVAVMGPSGSGKSSMMNLIMRFYEPTEGHLRIDGIDVRDVA
                     LRDLRKHISIVPQEITIFTGTIAENIMYGRFDAKPAQVIQAAKEAELHNFIMSMPEKY
                     ETYVGEHGTSLSGGQRQRLAIAMSLLTDPKILILDDSTSALDAETESRLRATLKRVMK
                     NRTSFVITHRIATARDADMIVVLENGRIAEVGTHEELIEREGAYCRIVELQQRGAVLI
                     EEDSPALS"
     gene            complement(1023..1928)
                     /locus_tag="P5179_07565"
     CDS             complement(1023..1928)
                     /locus_tag="P5179_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS95111.1"
                     /translation="MPSRFRVYVYVLVTVLTWSHAFVAIKYCLRYVSPWELLVLRHVP
                     AVLVFCVYLAARRSLRDLRRILRDDGVRFLLLGAAAVTGYHLSLNMGSQVIPSGTVSL
                     VIGSSPVFTLVMAALLIRERPALWQISGVVIAFAGLYACIRFGGGKDVDLRYVSGALV
                     LLLSPFFASIHTTLSRPLSRKYGALNTTAFTIILGTVPLLVGADADLVHKAAALPAGF
                     WAADLFLGLGCTAAAYLLWTKALQVMEATKVAVFLYLIPVLGIAWGWLYLDEPVSWWL
                     VAGAALVIFGVVLANRKAVAPAPVN"
     gene            2092..3432
                     /locus_tag="P5179_07570"
     CDS             2092..3432
                     /locus_tag="P5179_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS95112.1"
                     /translation="MPRKSTRLPNIVFFGVDSLRSDHMSAYDYHRLTTPHIDRLADEG
                     VLFEKMFSPNIPTPPGYIPMLTGRDLFSTQVVSLHHKDPLDPAIPTLPEILRDFGYSS
                     ACIGFGPGLYRGFDEYAEYRAWMNWEERPGDKAGKLNEVTMPILNRMAASNKPFFLFM
                     RHMDPHSPYLPPHPYDRMFYTGDECDPNNKSMEPVFAFAPFADFFRSWMPPGITDIEY
                     VNASYDGALAYMDGCIQQLLNRLDELGIVDETIVVFTADHGETLDEHDCYFDHHGLYE
                     PTLTVPLIIRYPGRLPEGVRVPGHTQLKDIVPTLMELIGIRPKVKFDGMSQIPLLTGD
                     RATNATEFYITECTWMRKHGIRTTEWKFFEALEPDFHNKPPVELYNLVEDPLELNNLA
                     DKEPGMVKELRRRMNAWIKKRTKQTGKGNPILDYHIGTDRRIGSVATANKLQKK"
     gene            3540..4907
                     /locus_tag="P5179_07575"
     CDS             3540..4907
                     /locus_tag="P5179_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95113.1"
                     /translation="MLTLTSRPYLFVDDQFIDTADSVRIELHHPVRQNVAFSFDAPWE
                     GPSSTYCTVLDIAAGNDSGTCPFEQPRYRLYYRGMAGDTDLVGECTCVAESEDGIAFR
                     RRNTWKFPACGSTANNIIWAGQNDISHNFAPFVDTNPSCTPEVRFKAIGGVSRHKVFA
                     LASPDGLDWHLFHDDPVLTEGDFDSLNVAFWDAANGWYAAYVRKSHGGFRSIDRCISE
                     DFIHWSKPEPLDLGPGELEHLYTNAIRPYARNASLYLGFPMRFFPHRTRFDSLAEKGV
                     SDAVLIASRDGLHFPRHFREAFVRPGLDQRNWTQRNLITAAGMIQTSPSELSIYWVEH
                     YDHHDCNLMRGTLRVDGFTSLHGGVPGGSAVSRPLKLEGTTLQANLSTSAGGSVKIGL
                     CDETGKPLEGFSIEDCDDLFGDDLARTVCWKGNRDLSRVKGAMVRLHLMLRDADVYSL
                     SAVDG"
     gene            complement(4908..5603)
                     /locus_tag="P5179_07580"
     CDS             complement(4908..5603)
                     /locus_tag="P5179_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS95114.1"
                     /translation="MLLRPDVIFFDYGNTLVKDTEDWFGDISRYLAPLGMELTEDRFR
                     IGKAAAEHYAGSYRQKNGTRTRLEDRFWFNYCKAFLEAAFGDGAGRLAEEMHAVQFFT
                     NEIYPDTHAVLRELRRRGYRLGVISNWEAPTLPSLFERFQLTQYFEKILPSRDAEASK
                     PDPLIFQKAMSALAVKPEDAVHVGDSFPCDVEGAQGVGITPVWLNEKGGAAPDGSSVL
                     QIRNLSGLLSLLA"
     gene            complement(5608..6306)
                     /locus_tag="P5179_07585"
     CDS             complement(5608..6306)
                     /locus_tag="P5179_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95115.1"
                     /translation="MAYLSIAFASFCGLLAGFFNPLEPGFWVPHLRSELHDKKTAGRA
                     SRILVAAVGQALTTSFVALLVTVLAFATIAFADQYTPLLGGGLVCVFAVFQFVRRRTG
                     APVDSGKIKELLHSIAAGSGRDPFGACEKTGWGTVQSAVFSPGFVLSPFFLAAAASGL
                     PSVTNAFSVVIAYVLGNALGAFFALRGIQAKIAGADKKTSPSDDVPNRVADQRKALTA
                     FATFVSGILAMLSG"
     gene            6547..7740
                     /locus_tag="P5179_07590"
     CDS             6547..7740
                     /locus_tag="P5179_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95116.1"
                     /translation="MVSSIPDWHGFPLTMHETIISRLARAYERDRLGHCLLLTGGGGN
                     GAYHVAVRLAQMFLCPSWDEPNGCQTCQNIGKLNHPDLSVLSPLPPRFGEGSSSGTPD
                     AEPVSTALSEDLFAPLNVGANWKITSEQADRLITWAWQTPWQAPRKVAIIAEADFITE
                     LIAPKLLKTLEEPPPAVTFLLITSRPQNLLPTVRSRCQVVPIPPLTEDEIVRILVEHG
                     ADEGDARSAAMLANGDVWVAQSLTRAGAEGTRRNAYNLIETALDPKRKPVHIFDLCRK
                     LLAPPEGKTRWLPSEEAAIVGEITRWMAWGLRNILVNLEESSEVPEELRSIFPSAKRI
                     GFARLFSWLDEVDRIHEMSIRNVSAMPALFALAMYLRDDRRVFRETDGSEIAAFPSLT
                     WWPSL"
     gene            7759..>8093
                     /locus_tag="P5179_07595"
     CDS             7759..>8093
                     /locus_tag="P5179_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95117.1"
                     /translation="MKNHDIEPDEKPLEELEDRDLHLQAEEEAPVEESAELEGEIVEV
                     VFRGNRRAVYVNAHNVQLARGNHVIVEADKGEDLGRVCQTGGKTRAGRRAKKKTVLRL
                     AESHEISRW"
BASE COUNT         1885 a         2377 c         2248 g         1583 t
ORIGIN      
        1 ccttgcaagc cttatcagcg tgttcggcac atccctgatt ctgtatcttg gggcgcttta
       61 cgttcgcgac cgcgtgataa ccgtgggaga ccttctgtat ttccacagtt cggttgccaa
      121 tcttttcagc cccgttgtcg tcctgacagg gctcaacgca accgtccagt gggtgctggt
      181 tgtgctgagg agagtgtttg atatcctcga tgaaccaatt accacctctg atccccccaa
      241 tcccgtcccg ctgaccagtg tccgcggtga ggtcgaattc gatcacgtga gcctgcgcta
      301 tcccaacgcc accacaaatg cgctcacaga catcaacctg aaggttcccg caggtaccac
      361 tgttgccgtt atgggcccgt ccgggtctgg caagagctcc atgatgaacc tgataatgcg
      421 gttctatgaa ccgactgaag gacacctgcg cattgacggt atcgacgtgc gggacgttgc
      481 gttgcgtgac ctgcgaaagc atatcagtat cgttccacag gagatcacca ttttcacagg
      541 tacgatcgct gaaaacatca tgtacggcag gtttgatgcg aagccggcgc aggtcataca
      601 ggcggcgaaa gaggctgaac ttcacaactt catcatgtca atgccggaga agtacgaaac
      661 atacgtcgga gaacacggaa ccagcctttc aggagggcag aggcagcgcc ttgcgatcgc
      721 catgtcgctg ctgacggacc cgaagatcct cattctcgac gacagcacgt cggcgctgga
      781 cgcggaaact gaaagccgcc ttcgagccac gctgaaacgc gtcatgaaga accgcacgtc
      841 gtttgtgatc acgcaccgca tcgccactgc gcgcgacgca gacatgatcg tggtcctgga
      901 aaacggcaga atcgctgaag tgggaacaca cgaggagttg atcgaacggg aaggcgctta
      961 ttgccgaatt gttgagctgc aacaacgcgg ggccgtactg atcgaggagg actctcctgc
     1021 tctcagttga ccggcgccgg cgcgacggcc tttcgatttg ccagaacgac tccgaatatg
     1081 accagcgctg cgcccgccac cagccaccac gaaaccggct catccaggta caaccatccc
     1141 caggcgatgc caagcacggg aatgagatac aggaacacag ccactttcgt cgcttccatc
     1201 acctgcagcg ccttcgtcca gagaagatac gcggcggcgg tacaccccag gccaagaaaa
     1261 aggtctgcgg cccagaatcc tgccgggagc gcggcagctt tgtggaccag atctgcgtcc
     1321 gcccccacga ggagaggcac ggtgccaaga ataatggtga acgcggtggt gttcaacgcg
     1381 ccatacttcc gggaaagcgg gcgactcagt gtggtatgta ttgacgcaaa gaagggggaa
     1441 aggaggagaa ccagcgcgcc ggaaacatac ctcagatcaa cgtccttgcc tcccccgaaa
     1501 cggatacacg cgtacaagcc cgcaaacgcg ataactacgc cggaaatctg ccacaaggcg
     1561 ggacgttcac gaatgagcag cgccgccatg accagcgtga acaccggaga ggaccctata
     1621 acaaggctca ccgttccgct gggtatgact tgcgaaccca tgttaaggga gagatggtat
     1681 ccagttaccg cggcggctcc aagcaaaagg aaccgcacgc cgtcgtcccg cagaatccga
     1741 cgtaggtcgc gtaaggacct gcgggcagcg agatacacgc agaacacaag aactgccggc
     1801 acgtgccgga gcaccagaag ctcccacggg ctgacgtaac gaaggcaata cttgatcgcc
     1861 acgaacgcgt gcgaccatgt aagaactgtc acaaggacgt agacgtatac gcgaaaacgg
     1921 gaaggcattc gggggttccc ggcgggttgt cgtacggcga acaaatctgt ttgaaaatct
     1981 acggagctcc gctggtgtcc ccaaatgctt ctcgggtcgc ttgcactgac ccgtttgcgc
     2041 tgcgatactt gacaagaata gttctcacac ccgcattaaa aggagattgc catgcctcgg
     2101 aagagcacac ggttgcctaa catcgtcttt ttcggtgtgg acagcctgcg gtctgatcac
     2161 atgagtgcgt acgattacca tcgtttgacc acgccccaca tcgatcggct cgccgacgag
     2221 ggtgttctct tcgagaagat gttcagcccg aacatcccta ctccgccggg ttacatcccg
     2281 atgcttacgg ggagagacct gttcagtacg caggtggtat cgctgcatca caaggacccg
     2341 ttggaccccg ccatcccaac cctgccagag atcctcaggg atttcgggta ttcatccgcc
     2401 tgtatcggtt tcggacctgg tctttatcgc gggttcgacg aatacgcgga atacagagca
     2461 tggatgaact gggaagagcg ccccggagac aaggcaggaa aactcaacga agtgaccatg
     2521 ccgatcctca accgcatggc ggcctcgaac aagccgttct tccttttcat gcgccatatg
     2581 gatccgcatt cgccgtatct cccgccccac ccgtatgacc gcatgttcta cacgggagac
     2641 gaatgcgacc cgaataacaa gagcatggaa ccggtattcg cgttcgcgcc gtttgcggac
     2701 tttttccgtt cgtggatgcc acccggcatc acagacatcg aatacgtcaa tgcgagctac
     2761 gacggcgcgc tggcgtacat ggacgggtgc atccagcagt tgttgaaccg tcttgacgaa
     2821 ctcgggatcg tcgacgaaac gatagtggta ttcaccgcgg accacggaga gactctcgac
     2881 gagcatgatt gctacttcga ccatcacggt ctgtacgagc cgactctcac agtccctctt
     2941 atcatccggt acccaggccg tcttcccgaa ggcgtgcgcg ttcccgggca cacacaattg
     3001 aaagacatcg ttccgacgct gatggaattg atcggaatcc gtcccaaagt caaattcgac
     3061 gggatgagcc agatccccct cctcacggga gaccgcgcga cgaacgccac ggagttttac
     3121 atcaccgaat gcacctggat gcgcaagcac ggcattcgta caaccgaatg gaagttcttc
     3181 gaagcactgg aaccggattt tcacaacaaa ccgcctgtgg agctttacaa tctggtggaa
     3241 gacccgcttg aattgaacaa tctcgcggac aaagagcccg gcatggtgaa ggaacttcgg
     3301 cgccgaatga atgcatggat caagaagcgg acgaagcaaa cgggaaaagg taatccgatt
     3361 ctcgactacc acatcggtac agaccgaaga atcggttccg tggccactgc aaacaaactt
     3421 cagaagaaat aggcgagaaa ccacgcatcc gggaggcgtc tgcggacgtc tcccggagtt
     3481 ggtaaccacg cgctacaccc ctttcgtacg tattccttgc ctttcaaagg gacccgaccg
     3541 tgcttacttt gacctcccgg ccgtatctgt tcgtcgatga ccaattcatt gacacggcag
     3601 attcggtgcg catcgaactc catcatcccg tccgccagaa tgtggcgttt tcattcgacg
     3661 ccccgtggga gggtccgagc agcacctact gcaccgttct ggacatcgct gccggaaatg
     3721 attccgggac ctgcccgttc gaacagccgc ggtaccgtct gtactatcgc ggtatggctg
     3781 gagacacgga ccttgtgggc gaatgcacgt gcgtggccga aagcgaagac gggatagcat
     3841 tccgccggcg aaacacctgg aagttccccg cctgcggatc gaccgcgaat aacatcatct
     3901 gggccggaca aaacgacatc tcccacaact tcgccccgtt tgtcgacaca aacccctcgt
     3961 gcactcctga ggtccgtttc aaagccatag gaggggtttc ccgtcacaag gtgttcgcgc
     4021 tggcgtcccc cgacggactc gactggcacc tgttccatga cgatcccgtg cttacggaag
     4081 gcgatttcga ttccctgaat gtggctttct gggacgctgc aaacgggtgg tacgcggcat
     4141 acgtgcgaaa atcgcatgga ggatttcggt caatcgatcg ctgcatttcg gaggatttca
     4201 tccactggag caagcctgag ccgctggatc tggggccggg ggaactcgaa cacctgtaca
     4261 caaatgccat ccgaccttac gcgagaaatg cgagtctata tcttggtttt ccgatgcgct
     4321 tctttccaca caggacccgg ttcgattctt tggcagagaa gggcgtcagc gacgccgttc
     4381 ttatcgccag ccgcgacgga ttgcatttcc cgagacattt ccgcgaggcg tttgtccggc
     4441 cgggtctgga ccagcgcaat tggactcaac gcaacctgat aacagccgcc ggcatgatcc
     4501 agacgtcacc ctcagagctc tcgatctatt gggtggagca ttacgatcat catgactgca
     4561 acctcatgcg cggcacgctg cgcgtggatg gtttcacttc gctgcacggc ggtgttcccg
     4621 gcgggtcagc ggtctccagg ccgctcaaac tggaaggcac cacacttcag gccaatctct
     4681 ccacatccgc gggtggcagc gtgaaaatcg gcctgtgcga cgaaaccggg aaaccgttgg
     4741 aagggttttc gatcgaagac tgcgatgacc tgttcgggga cgatctggcc cgtaccgtct
     4801 gttggaaagg aaaccgcgac ctttcacgcg tgaaaggtgc catggtccgc cttcacctca
     4861 tgttgagaga tgcggacgtg tattcccttt cggctgtcga cgggtaatca cgcgagaaga
     4921 gagaggaggc cggacaggtt ccggatctga agaaccgatg acccgtccgg cgccgcgcca
     4981 cccttctcgt taagccagac aggggtaatc cctactcctt gcgcaccctc cacatcacag
     5041 gggaagctat cccccacgtg caccgcatcc tcgggtttca cggcaagagc agacatcgcc
     5101 ttctggaaaa tcagcgggtc gggtttggat gcttccgcat ctcgggaagg gagaatcttt
     5161 tcaaaatact gcgttaactg gaaccgttcg aacagagagg gcagcgttgg ggcttcccag
     5221 ttggagatca caccgaggcg atatcctctt cgacgcaatt cacgtaacac ggcgtgggta
     5281 tcggggtaga tctcgttagt gaagaactga accgcgtgca tttcctctgc cagccgtcca
     5341 gctccatcgc cgaaagcggc ttcaagaaac gccttgcagt aattgaacca gaaacgatct
     5401 tcaagcctcg tgcgcgttcc gttcttctgg cggtaactgc ccgcgtagtg ctctgcggca
     5461 gctttcccaa tccggaaccg gtcttccgtc agctccattc ccaggggggc caggtagcgc
     5521 gaaatatccc cgaaccagtc ttccgtatcc ttgacgagag tattgccgta atcaaaaaag
     5581 atcacatcag gccgcagaag catggagtca ccccgaaagc atcgccagaa tcccggacac
     5641 aaacgtcgcg aacgcagtta atgccttcct ttgatccgcc acccgattgg gcacgtcatc
     5701 ggacggactc gtctttttgt ccgcacccgc gattttggct tggatccctc gaagggcgaa
     5761 gaaggcgccg agcgcgtttc cgaggacata cgcaatcacc acggagaacg cgttggtgac
     5821 cgaaggaagc ccgcttgccg ccgctgcaag aaagaaagga gaaagcacga acccgggaga
     5881 aaaaactgcc gactggacag ttccccaccc tgtcttttca caggctccga atggatctct
     5941 gccggaccct gcagcaatcg agtgcaacag ctctttgatt ttccctgaat caacaggggc
     6001 gcctgttctt ctccgcacga actggaaaac cgcgaaaacg cacaccaatc caccaccaag
     6061 aagaggcgtg tattgatcag cgaaggcgat cgtggcgaac gcgagaacag tcaccagcaa
     6121 cgccacgaat gatgttgtca gcgcctgtcc cacagcagcc acgagtatcc gcgatgcgcg
     6181 ccccgcggtc tttttgtcgt gcagttcgga acggagatga ggcacccaga agcctggttc
     6241 cagaggatta aaaaaacctg cgagcaatcc gcagaaggag gcaaatgcga tggagagata
     6301 agccacgttg gtcctctgtc cgttgtgaga gcagaatcac ggcacggaat tgacgctgta
     6361 gtaagattac gttcttcgga tagacggacc aacgaaccgc agccccggcc ggtgtgtcgg
     6421 cccggcagga acctcacgtg caatgcggca ccttacacag caaaaaccgt tcgcggctct
     6481 gcgcgaaccg gcgccggctc tttcctgcca cgcgagtccg ctttacttta caaaacggcg
     6541 tatttcgtgg tttcttcgat tcccgactgg cacggattcc ctctgacaat gcacgaaacg
     6601 atcattagcc gcttggctcg tgcatacgaa cgcgaccgcc tcggtcattg cctcctcctc
     6661 accggtggag gaggcaacgg agcataccat gtcgccgtcc gcctggcgca gatgtttctc
     6721 tgcccttcgt gggatgaacc gaacggttgc cagacctgcc agaatatcgg gaagctgaac
     6781 cacccggatc tctcggtgct gtcgccatta cccccacgtt tcggcgaagg aagctccagc
     6841 ggtacccctg acgccgagcc ggtgagcacc gcactgtcag aggatctgtt cgcgcccctc
     6901 aacgtaggtg caaactggaa aatcacttcc gaacaggccg atcggctgat tacgtgggca
     6961 tggcagactc cgtggcaggc gccgcggaaa gtagcgatca ttgcggaagc tgacttcatc
     7021 accgagctga tagccccaaa acttctcaaa acgctggaag agcctcctcc tgcggtgact
     7081 ttcctactca taacgtccag gccgcagaac cttcttccaa ccgtccgcag ccggtgtcag
     7141 gttgttccga tcccaccgct cactgaagac gagatcgtac ggatcctcgt tgaacacggc
     7201 gccgatgaag gcgatgcccg gtctgccgcc atgctggcca acggagatgt gtgggttgcg
     7261 caatcgctca cacgcgcggg agccgagggg acgagacgca acgcgtacaa cttgatcgaa
     7321 actgcgcttg acccgaagag gaagcctgtg cacatatttg acctttgccg aaagcttctc
     7381 gcccctccgg aagggaaaac ccggtggctt ccttctgagg aagccgcaat cgtgggggaa
     7441 attaccagat ggatggcgtg gggtctccgc aatatccttg tgaatctcga ggaatcatcg
     7501 gaagtgcccg aagaactacg cagtattttt ccttcagcga aacggattgg tttcgcccgg
     7561 ttgttttcct ggctggacga agtcgacaga attcatgaga tgtcaattcg taacgtgagc
     7621 gcgatgcctg cccttttcgc gctcgcaatg tatttgcgcg acgacaggcg ggtcttccgg
     7681 gaaacggacg gctcagaaat tgccgcattt ccctctctga cctggtggcc ttctctttga
     7741 tcggcaacgg aatctcgaat gaaaaaccac gatatagagc ccgatgaaaa acccctcgaa
     7801 gaactggagg atcgcgatct ccacctccag gccgaggaag aagcgcccgt agaggagtcc
     7861 gcagaacttg aaggagagat agttgaggtg gtttttcgcg gaaaccggcg ggccgtgtac
     7921 gtcaacgcac acaacgtgca gcttgcgcgc ggaaaccacg ttatcgtcga ggcggataag
     7981 ggagaggatc tggggagagt gtgccagaca ggaggcaaaa cgcgcgcagg acgacgggca
     8041 aagaagaaaa ccgtcctccg attggcggaa tctcacgaaa tctcccgctg gaa
//
LOCUS       DAOTNG010000130         8086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_7966_length_8086_cov_5.392853, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000130 DAOTNG010000000
VERSION     DAOTNG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7966_length_8086_cov_5.392853"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1290
                     /gene="ribD"
                     /locus_tag="P5179_07600"
     CDS             199..1290
                     /gene="ribD"
                     /locus_tag="P5179_07600"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRS95118.1"
                     /translation="MRERQEARFMDEALALAAKGRGYTSPNPMVGAVVVKNGTVIGRG
                     YHRQAGLAHAEVVALDEAGEAARGATMYVNLEPCSHHGRTPPCTDRIIAAGIERLVCS
                     HIDPDERVNGRGIELLRKAGIDVQVGLREREARRLNEHFLHWKTTGTPWLTLKWAQSL
                     DGRVATSTGNSQWITGEEARAHAHRLRSWHDAVLIGVGTALADDPRLTVRMVEGHQPY
                     HVVLDPGLRLSPNARLVEPGNTVILCAESADPQRIAAWERNDVQAVPVRSHGDVLEIE
                     AVLEALTALRFQSVFVEGGSRTLTSFLKAGAANRLTVFIAPIILGEGIPAVGDLGIET
                     LDQAIRLREVEVERFGNDLCITGLCGAGP"
     gene            1290..1949
                     /locus_tag="P5179_07605"
     CDS             1290..1949
                     /locus_tag="P5179_07605"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRS95119.1"
                     /translation="MFTGIVEAVGTVTRVVPSGGVQHTEVACPEVMDDVALGDSIAVE
                     GVCQTVTGRTADTFTFDSVAETLRLTTIGSWRTGRRVNIERSLKVGARLGGHWVLGHV
                     DGVVRLLERKNLGDTIEIRCSLPSQLLPQVALKGSVALDGVSLTVTSVNDSSFGVAVI
                     PFTLEHTTLGGRKTGDEMHLETDVLAKYVSRLLESATPGGKASDRNIGALLREAGFMD
                     T"
     gene            1946..3175
                     /locus_tag="P5179_07610"
     CDS             1946..3175
                     /locus_tag="P5179_07610"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRS95120.1"
                     /translation="MIAVDEAGVFATIGEALDELRAGRMVIVVDDEGRENEGDFVMSA
                     QMATPDAINFMVTHARGLVCLAAEQSRLQQLGLELMVPSPNNTAAHGTAFTVSIDAVH
                     GTTTGISAGDRATTIRKFVDPAAKPQDFAKPGHVFPLAAAKGGVLRRAGHTEAAVDLA
                     RLAGHTPAGVICEILNEDGTMARVPELVLMAKRMGLKIVTIKDLIEHRRRSECLIRRV
                     EEVQMPTEYGDFLLILYESLLDQEFHIALVKGDVRTEDPVLVRVHSQCLTGDVFGSAR
                     CDCGAQLAWALRKIEAEGRGAVVYMRQEGRGIGLENKLHAYSLQEIGLDTVQANQFLG
                     FPPDIRDYGIGAQILRDLGIQRVRLMTNNPAKRAGIEGYGLDVVEMIPILIPPNPHNE
                     RYLHTKRTKLGHLLEYE"
     gene            3270..3740
                     /gene="ribE"
                     /locus_tag="P5179_07615"
     CDS             3270..3740
                     /gene="ribE"
                     /locus_tag="P5179_07615"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRS95121.1"
                     /translation="MTYEGQLSAEGHRFGIVVSRFNAFISEHLLTGCLDAIKRHGGDL
                     DKVDVVWVPGSFEIGVTARRLAKTNAYNAIICLGAVIRGSTPHFDYVANEAAKAIAQV
                     GLETGVPTIFGVLTTDSVEQAIERAGTKMGNKGADAALAAIEMASLFACLPTSE"
     gene            3719..4168
                     /gene="nusB"
                     /locus_tag="P5179_07620"
     CDS             3719..4168
                     /gene="nusB"
                     /locus_tag="P5179_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRS95122.1"
                     /translation="MSTDLGVKRESRHAARKIAVQTLYWLQSQPWDNVSDIVQNLGDE
                     EHVAEQSRERALLLCRHAEEKRPEFERLAASACENWEIERVGRVEQVIIRLALAEWDL
                     KDVPPRAVLSEAVNLAREFCGDRAAGFVNGVLDRIARETGVLAPPDK"
     gene            4451..7462
                     /locus_tag="P5179_07625"
     CDS             4451..7462
                     /locus_tag="P5179_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95123.1"
                     /translation="MPRLPASISRLVFVIIALSLSGPALFAQTPVSRREADDYTFARR
                     LYDQGDYDLAERQLRDFLTRYPASPRRADVRFMLADCDFNRGRYATALEQFERFRIEF
                     GASPHDDDALFRIGQCLFRLARYQQAADAFSRLLLAYPSSNLRYEAEMFLGEARFELG
                     QLEAAARSYEQAAQGTGKVVAHALYSLGWVRQKQGNYEEAISAYQRMLQNDPSSALAS
                     QARFKLGECLYQQKRYQEAVSALSQALPRLSDPQLRANANYLLGESYRALGQYARART
                     YYQTITSEYRTTSLAPHSQYAIGLTYVEERNLQEAQRAFAEVTAKFPESAIVPNAEFR
                     RAIVLKDLGNRSEAERIFSLVATRYPSSDVADDALYELGVLAYNRMAYEEAERSFRRI
                     TYDYPGSDLRPQATYMMGEIALARGTSDSAEVYYSRFIGSYPTHSLLPNAFFQRGSTR
                     FKQGRYQLAIDDLTQVVRRWTSHALVPDAYFLVAESRYALGDYAGAAANYQLVIDRFA
                     QSPLVAQAYYGLGWCAFKTRDWAKAARAFERAKTLATDPRIKSDAAVRIGDSYYNQAN
                     FTRALAAYREASEINNSDPRAVYYAAQSLFQTGRLREAISLYEQVTRRFPESDLADDA
                     WYMIGWSRLRMDDYEGAISAYRHLVLHYPSSEKAPEAQLGLGDAYYNSGRFSDAITAY
                     DDVLRRWPNSEQVQGAVTGLWDSYLRQGKIDEAIERTERFIQANPRKPASASLLMKRA
                     DWFYSAGRYEDAVQAYRRIADEFEGVSLPTSPVFWEASALAQLGRRDQAIARFRVVSL
                     EPDNPLAPDALERIGDLQREAGALQAAVSSYQEYERRFPNSSKRLAVLAKLGDAQRDL
                     GNEAAAEQAYRTAAQAGPASAAGAQASLSLAELLLKRGGYDQARRLADSVLVSRSDES
                     AARAQFILGEIAFAQRKFEDARLAYMRVKLIYSSFPDWVARAQIRIADCYREMGNRAA
                     ARDSYREVLNTHPSDSLGIRARRQLDLLGGR"
     gene            7465..>8086
                     /locus_tag="P5179_07630"
     CDS             7465..>8086
                     /locus_tag="P5179_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95124.1"
                     /translation="MARGYARSAGAFASILVVLAGLPNLATGQAPETPVLDIPDSLVI
                     QGRVQHELLLAGTKSNTLYDRAFPVEREDVPEQLASVNTLPAISSKETFPLFIRHGSV
                     HATALRAAAQHFGNGSLWVGRWQDWKGFLLSSDLELGGGRGHTDNASWWRTALGTNTR
                     YMPSWRTACDASLDIESGRTAIWDGVLSSHRNYSRGGLGLALNRGLS"
BASE COUNT         1606 a         2441 c         2504 g         1535 t
ORIGIN      
        1 tgcttccgcc accggtacac ccccctgtga tgtccttcag cgccgtattg cccccccaat
       61 ctgtgcgcaa aactggtttt cacgtcattt gacctgtaaa atatacggtt gctcgttgag
      121 cgagttcgcc gtacggaatc cgcacttatg atgaattcgt cccggtccac caggccagcc
      181 ggctcttccg gcgtacgtct gcgggagcgt caggaagccc gtttcatgga cgaggccctc
      241 gcgttggccg cgaaggggag aggatacacg tccccgaatc cgatggtcgg tgcggtcgtg
      301 gtcaagaacg gaaccgtcat cggccggggg tatcatcggc aggcgggtct cgcacacgcg
      361 gaagttgttg ccctggatga ggcgggtgaa gccgcccgag gtgcaacgat gtatgtgaac
      421 ctcgaaccgt gctctcatca cggccgcacc ccaccatgca ccgatcggat tatcgccgcg
      481 gggatcgaac gcctcgtctg ttctcacatt gacccggacg agcgcgtgaa cgggcgtggc
      541 attgagctgt tgaggaaggc cggtatcgac gttcaggtgg gcctgcgcga gcgtgaggct
      601 cgcagactga acgaacactt cctgcactgg aaaacaaccg gaacaccgtg gttgacgctc
      661 aaatgggcgc agagccttga tggacgcgtg gcgacgtcta ccggcaattc tcagtggatt
      721 acaggtgaag aagcacgcgc gcacgctcac cgtttgcgat cctggcatga tgcggtgctg
      781 atcggcgtgg gaaccgcgct ggccgacgat ccccggctca cggtgcggat ggtggaaggc
      841 catcagccct accacgtggt tctggacccg ggtctgcgtc tttcgccgaa tgcccggctt
      901 gtggagcccg gaaacaccgt gatcctgtgt gcagagtcgg ccgatccgca acgcatcgcc
      961 gcatgggaaa gaaacgatgt tcaggcggtg ccggtcaggt cacatggcga cgtgctcgag
     1021 attgaagctg ttcttgaagc tctgacggca ttgcggttcc agagtgtatt tgtggaaggg
     1081 ggctcgcgca cgctgacgag cttcctgaaa gccggcgcgg cgaaccgcct gacggtattc
     1141 atcgccccga tcatccttgg cgagggcata ccggcggtcg gtgacctggg aatcgaaacg
     1201 ctcgatcagg ccatacgcct gcgtgaagtc gaggtcgagc ggttcgggaa tgatctctgc
     1261 ataaccggcc tttgcggagc gggaccctga tgttcaccgg aatcgtcgaa gcagtcggaa
     1321 ccgtcacgcg cgtcgtccct tccggcggcg ttcagcacac ggaggtcgcc tgcccggaag
     1381 tgatggacga cgtcgccctc ggtgacagca tcgcggtgga aggtgtatgc cagactgtca
     1441 cgggtcggac ggcggatacg ttcacgttcg attccgttgc ggaaacgctt cgtctcacca
     1501 ccatcggttc atggcggact ggaaggcggg tgaacatcga acggtcgctc aaagtcgggg
     1561 ctcggctggg cgggcattgg gttctggggc acgtggatgg agtagtccga ctccttgagc
     1621 ggaagaacct cggggatacc atcgaaatcc ggtgctcgct gccatcccag cttctccctc
     1681 aggtcgcact gaaaggatcc gtggcgctcg acggggtgag cctgacggtc acgtcggtga
     1741 acgattcgtc cttcggagtc gccgtaatcc cgttcactct tgaacacacg acactgggcg
     1801 gcaggaagac aggcgacgaa atgcacctcg aaaccgatgt tcttgcgaaa tacgtctccc
     1861 gcctgcttga aagcgcgact ccgggaggca aagcgagcga caggaacatc ggagcgcttc
     1921 ttcgggaagc ggggtttatg gacacatgat agcagtggat gaagcgggcg tttttgccac
     1981 gattggcgaa gcgctggacg aattgcgcgc cggccgcatg gtcattgtgg tggacgacga
     2041 gggccgcgag aacgaaggcg acttcgtaat gtcggcgcag atggcaacac cggatgccat
     2101 caatttcatg gttacccacg cccgggggct ggtgtgcctc gccgcggagc aatcccgtct
     2161 gcaacagctc ggtctcgaac tcatggtccc gtctcccaac aacaccgcgg cgcacgggac
     2221 tgcattcacc gtcagcattg atgcggtcca cggtacaaca accggaatat cggcgggtga
     2281 ccgcgcgaca acgattcgca agttcgtgga tcccgccgcc aaaccgcagg atttcgccaa
     2341 gccggggcat gtgttccccc ttgcggcggc aaaagggggt gtcttgcgcc gcgcggggca
     2401 taccgaggcc gccgttgatc tcgcgcgcct cgccgggcac acgccggccg gggtgatctg
     2461 tgaaatcctg aatgaagacg gtacaatggc gcgcgttcca gaacttgtcc tgatggcaaa
     2521 acgcatgggc ctgaaaatcg ttacgatcaa agacctgatc gaacaccggc gccgaagcga
     2581 atgcctcatc cgccgcgttg aagaagtgca gatgccgacg gagtacggcg actttttgct
     2641 tatcctttac gaaagcctgc tcgaccagga attccatatc gctcttgtga aaggtgatgt
     2701 tcgtacggag gacccggtcc tcgtgcgagt acattctcaa tgtctcacgg gggacgtatt
     2761 cggcagcgcc cggtgtgatt gcggagccca gcttgcgtgg gcgcttcgaa aaattgaagc
     2821 cgaaggcaga ggcgcagtcg tctacatgcg acaggaggga cgcggtatcg gtctggaaaa
     2881 caagctccat gcgtacagcc tgcaggaaat cggtctcgac accgtgcagg cgaaccagtt
     2941 tctcgggttt ccgccggata tcagggatta cggcatcggt gcacagattc tgcgcgatct
     3001 gggcattcag cgcgtgcgcc tgatgaccaa caatccggca aaaagggcgg gtattgaagg
     3061 atacgggctt gatgtggttg agatgatacc gatactgatc ccgccgaatc ctcataatga
     3121 gcggtatctc cacaccaagc gcactaaatt aggacacctg ctggaatatg agtgacgaca
     3181 acccatccaa cgaggaaatg cctcccatat cactgggatc ttctgatgaa tcaacggaga
     3241 gttgttcccc ttctgagaag gacggaacca tgacatacga aggccaactg agcgccgaag
     3301 gacatcgctt cgggattgtc gtaagccgct ttaacgcgtt catcagcgaa catctgctca
     3361 cgggatgcct ggacgcgata aagcggcatg ggggagacct cgacaaggtg gacgtcgtct
     3421 gggtccccgg ttcgtttgaa atcggtgtca cggcgcggcg tttggcgaaa acgaacgcgt
     3481 acaacgccat aatctgtctc ggagccgtca tccggggctc cacgccgcat ttcgactacg
     3541 ttgccaacga ggcggcaaaa gcgatcgcgc aggtgggtct ggaaaccggc gttccgacga
     3601 tctttggcgt tctcactacc gactcggtcg agcaggcgat tgagcgcgca ggcacgaaaa
     3661 tggggaataa gggtgccgat gcggccctcg cagcaatcga aatggcaagc ctgttcgcat
     3721 gtctaccgac ctcggagtaa agcgggaaag ccgccacgcg gcacgaaaga tcgcggtgca
     3781 aaccctgtac tggcttcaga gccagccgtg ggacaacgtc agcgacatcg tccagaacct
     3841 gggtgacgag gaacacgttg cggagcaatc acgcgaacgg gcgctcctgc tgtgccgtca
     3901 cgcggaggag aagcgccccg aatttgaacg cctggcggct tccgcctgcg aaaactggga
     3961 aatagaacgc gtcggacggg tggaacaggt aatcatccga ctcgcgctgg cggaatggga
     4021 tctgaaggac gttcccccca gagccgtcct gagcgaggcc gtcaatctgg cacgggaatt
     4081 ctgcggagac agggccgcag gatttgtgaa cggtgtgctc gatcgcatcg cccgggagac
     4141 aggcgtgctt gccccgcccg acaaatgacc cattgccgga cgcgatgatc ggtacaccgc
     4201 gtggaacaaa gacgtgcaac ctgcgtatgc agattgcacg ttcttgttat cgggccgtcc
     4261 cggccgttcg ggatggaagc cgcatgcgcg caatttcccc ggtggagttt cgacgcgagc
     4321 cctgtctgga ttcgccacgg agacgttgca ctcaattgcc ctaagttggc tcgaagtttg
     4381 cacctgacgt atcattgttg aatacagcag tccttatccg cgatcaggac acggagcgaa
     4441 ccgaccgcaa atgcctcgcc ttcctgcctc gatttcccgt ctggtgttcg tcatcatcgc
     4501 tctgtcgctt tccgggcctg cgttgtttgc gcagacaccg gtcagcagga gagaagccga
     4561 cgattacacc ttcgcgcgcc gcctgtatga ccagggcgat tacgacctcg ctgaacggca
     4621 gttgcgcgac tttctgacgc gataccccgc cagtccgcgc cgggcagatg tccggttcat
     4681 gctcgcggat tgcgacttca atcgcggacg atatgccact gcgttggagc agtttgaacg
     4741 cttccgcatc gagttcggcg catctcccca cgacgacgat gcgctgttca ggattggcca
     4801 gtgtcttttc cgtctcgcgc ggtaccagca ggctgccgac gccttcagcc ggttgttgct
     4861 ggcatacccg tcatccaatc tccgctacga agcagagatg ttcctgggcg aagcgcgctt
     4921 tgaactcggg cagcttgagg cggcggcgcg gagttacgaa caggcggcgc aaggcaccgg
     4981 gaaagtggtt gctcacgcgc tgtacagcct cgggtgggtg cgccagaaac agggaaacta
     5041 cgaagaagcg atttccgcat accagcgaat gcttcaaaac gatccctcaa gcgcgctggc
     5101 ctcgcaggcc aggttcaaac tcggtgaatg tctgtaccaa cagaaacggt atcaagaggc
     5161 agtcagcgcc ctctcgcagg cgcttccaag gctctcagat ccccagcttc gcgccaacgc
     5221 caactacctc ctcggcgaaa gctaccgtgc gctgggccag tatgcccgtg cgagaaccta
     5281 ttaccagacc attacgtcag aataccgcac cacctccctc gcgccgcact cccagtacgc
     5341 catcggcctc acgtacgtcg aggaacgaaa ccttcaggag gcgcagcgag cgttcgcgga
     5401 agtcactgcg aagttccccg aatcggcgat cgttcccaat gcggagtttc gcagggcgat
     5461 cgtgttgaag gacctgggca accgctccga ggcagaacgg attttctccc tcgtggcgac
     5521 gcgttatccg tcttctgacg tcgcggacga cgctctctac gaactcggtg tgcttgcgta
     5581 caaccgaatg gcatacgagg aagccgaacg gtcgttccgc aggatcacgt acgactaccc
     5641 cggcagcgac cttcgtccgc aggcgacgta catgatggga gaaatagcgc tggctcgagg
     5701 cacctccgac agcgcggaag tgtattactc ccggttcatc gggtcgtacc cgacccattc
     5761 cctgctcccg aacgcctttt tccagcgcgg ctcgacccgc ttcaaacagg gtcggtatca
     5821 gcttgccatt gatgacctca ctcaggtggt gcgccggtgg acctcccatg cgctggttcc
     5881 cgatgcgtat ttcctcgtcg ccgaatcccg gtacgcgctc ggggattacg ccggcgccgc
     5941 ggcaaattac cagctcgtga tcgaccgctt tgcccaaagc ccccttgtcg cgcaggcata
     6001 ctacggtctt ggctggtgcg cgttcaaaac ccgcgactgg gcgaaagctg cgcgcgcgtt
     6061 cgagagggcg aaaacactgg ccacagaccc tcgaatcaaa tccgacgccg cggtgcggat
     6121 cggcgactcc tactacaatc aggcgaactt cacccgcgct cttgcggcat atcgtgaggc
     6181 gagcgagatc aataattcgg accccagagc cgtctattac gccgcccaga gccttttcca
     6241 gaccggacgt ctccgcgagg ccatttctct gtacgagcag gtgacccgga gattcccgga
     6301 atctgatctt gccgacgatg cctggtacat gatcggatgg tcacggctgc ggatggatga
     6361 ctatgagggt gccatcagcg cctatcgcca cctcgtgctg cactacccgt cgagcgagaa
     6421 ggccccggag gcgcaactgg gcctcggcga cgcctactac aacagcgggc gattcagcga
     6481 cgcgatcacc gcttacgacg atgtgttgcg gcggtggccg aactctgaac aggtgcaggg
     6541 cgccgtcacg gggctgtggg attcctacct gcgccagggg aagatcgacg aggcaatcga
     6601 gcgcacggag cggttcatcc aggccaatcc ccggaaaccc gcgtctgcca gccttctcat
     6661 gaagcgcgcc gactggttct actccgcggg caggtatgaa gacgcggtac aggcctatcg
     6721 tagaatcgcc gatgagttcg aaggcgtgtc attgccaacc tcgccggtct tctgggaagc
     6781 cagtgcgctg gcccagttgg gaagacgtga tcaggcgatc gctcgcttcc gggtggtctc
     6841 gctggagcct gacaatcctc tcgctccgga tgctctggaa cggatcggcg acctgcagcg
     6901 cgaggctggt gcgctacagg cggcagtgtc gagctatcag gagtacgagc gccgcttccc
     6961 caacagctct aagcggctgg ccgtgctggc caaactcggc gatgcccaga gagatctcgg
     7021 caatgaggcc gccgcggagc aggcataccg cactgcagcg caggccggac ctgcctccgc
     7081 cgccggggcg caggcttccc tctcgctggc agagctgttg ctgaagcgcg gagggtacga
     7141 tcaggcacgc cgactcgccg attccgtcct cgtgtcccgg tcagacgaga gtgcggcgcg
     7201 ggcgcagttc attctcggcg agatcgcatt tgcccagcgc aaatttgagg acgcgcgcct
     7261 cgcgtacatg cgcgtcaaac tcatttactc cagctttccc gactgggtgg cccgggcaca
     7321 gatccgcatc gccgactgct accgcgagat ggggaaccgg gcggcggcac gcgattcgta
     7381 ccgcgaggtg ctgaacactc atccttcgga tagtctcggc atccgcgcac ggcgccagct
     7441 cgatctgctc ggaggacgct gaacatggcg cgggggtatg cgcggagtgc gggagcgttc
     7501 gcgtcaattt tggttgtcct cgcgggcctg ccgaatctgg caaccggaca ggcgccggag
     7561 acgcccgtgc tggatatccc tgattccctg gttattcagg gacgtgtcca gcacgagctc
     7621 ctcctcgcgg gaacgaaaag caacacgctg tatgaccgcg ctttcccggt ggaacgggag
     7681 gatgttccgg aacaactcgc gtcggtaaac acgctgcccg cgatttcatc caaggagacc
     7741 ttccctctct tcatacgcca cggctccgtg cacgcgacgg cgctcagagc agcggcacag
     7801 catttcggga acggttccct gtgggtgggc cgctggcagg attggaaagg tttccttctt
     7861 tccagtgatc tggagcttgg cggcggacgc ggccacactg acaacgcctc ctggtggcgc
     7921 accgctttgg gcacgaacac ccgttatatg ccctcctggc gcaccgcctg cgatgcctcc
     7981 ctcgacattg agtccggccg aaccgcaatc tgggacggcg tgttgtcctc tcaccggaat
     8041 tactctcggg gcgggctggg gctcgcactc aaccgcggcc tctccc
//
LOCUS       DAOTNG010000131         7990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8063_length_7990_cov_4.813233, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000131 DAOTNG010000000
VERSION     DAOTNG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8063_length_7990_cov_4.813233"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1527
                     /locus_tag="P5179_07635"
     CDS             334..1527
                     /locus_tag="P5179_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95125.1"
                     /translation="MSADAIPDHMKWWREATFGMFIHWGVYAVPARNEWVMYVENIPK
                     EEYAHFADEFTARHYHAREWVKLAQDAGMKYMVLTTRHHDGFCLFDSKVSDFTAPKTA
                     AKRDLIAEYADACHALGMRMGFYYSLEDWRFPHQLPHLPMREDKSVYQPMVEQAHAQV
                     RELMTNYGTVDILWYDGGFPAGIWRSEELNAMVRSLQPNIVINDRSGLPEDFGTPEQH
                     IAPQDRPWEACMTMNDTWGYTAGDKNYKSVRQILGMLIQCASQGGNLLLNVGPDAEGR
                     IPEPAVERLRVVGEWMRTNGKAIYGATRAPLVTHSFATPTRVGDRLYLLSAHWPGSTA
                     SFGWCGNRVLSAKLLATGERLIVEQKGDRVWLRGLPQYAPDVNVSVIEITVEGEPKWP
                     EVRFT"
     gene            complement(2260..3291)
                     /locus_tag="P5179_07640"
     CDS             complement(2260..3291)
                     /locus_tag="P5179_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRS95126.1"
                     /translation="MKAAVMRGIRDVRIEDIPVPPVLDEEALVRIRSVGVCGSDIHYY
                     THGKIGRYVVQPPFLLGHECAGEVVEVGAAVRNVRPGDLVAVEPGVPCRRCRFCREGR
                     YNLCPDVVFLATPPVDGAFAEYIAHSADFLFPLDGVSLHEGAMCEPLSTGMQAVRRSG
                     LRMGDTVFIAGVGPIGLCALQAFLAGGAAAVYVSDLDADRCRRAVALGATQAFDASDS
                     ELAAKLRDLTHGRGPDIVVETAGSEKATAQSVQIVRAGGIVVLIGLSPAAIIPIDTIA
                     IIDKEIDIRGVFRYANTYPAALSMIRRGAVDVKSMITAEYPLDRAKQALDDVAARKPG
                     VIKAMVVNG"
     gene            3493..5181
                     /locus_tag="P5179_07645"
     CDS             3493..5181
                     /locus_tag="P5179_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95127.1"
                     /translation="MIVHPGIPENLRWFDESRFGMFIHFGLYALLGRGEWVMYHENIS
                     RPEYRKLMTVFNPEKFSADEWVGLAKEGGARYITVTAKHHDGFCLFDSSLTDYKITNT
                     PFGRDLIGELVDACHRADMRIIFYYSQPDWYHPNFVHNRGAFKDLPHPPPTDKPDWEK
                     YVRYFHGQVRELCTKYGRIDGIWFDGSHKTVEEWRGKELYDMIKELQPNAVVNDRARW
                     GDFFTPERSLPDDLTGYMFEACESISPTAWGYQDDMPAHTVPHLVQSLVRMAAAGGNY
                     LLNVGPKPDGTIAEEQAAVMREIGKWLTKNGESIYRSHAGPRLEDPNIRVTRKGTTLY
                     LHLLEWPARNRIVLPNVIAEGVKEAALLGSGSELEVVETDEGAELRGLPIMPPDPLVN
                     VIRVEFFRLPEHFTFGAKEQAVAVPQPVSVSASSPTHLTPETARFEGLGVKGAKLRVR
                     RNEAGKAFISGWMVPDHAAHWDARVEESGRYRIDVVVAAPETHAGAVVKLIAGSTELT
                     FTAPETRSLDTVETVHAGTVELSAGVTRITLQPEKLKWGYLSPDIAEVVLTPVG"
     gene            5346..6221
                     /locus_tag="P5179_07650"
     CDS             5346..6221
                     /locus_tag="P5179_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS95128.1"
                     /translation="MAKQTGIGCERCERFGRLGYRLDTDRGQDNPTGRVVACECAREL
                     CTCGALHEPTFAPAPPYEQLPERAAAAEDETACICWRVRRHVTTLNRLLRDAGIPNLY
                     RGKLREDYHVRYPEGQVIPDAESARGQAVTWLETAVKEPTGKSLFLYGVPGDGKTLLA
                     SAFLTEIILLTRKSGLFVNLSRGYFQRLRNTYEDEPSIETTDQVFNRLTRVPFLVLDD
                     IGVERGSAWEIEWLYNLIDARYQNQNRLIVTSNNSLEELNVLSHGRISSRLRHACMVV
                     RLPDVDLRERFRYGA"
     gene            6221..7054
                     /gene="rsmA"
                     /locus_tag="P5179_07655"
     CDS             6221..7054
                     /gene="rsmA"
                     /locus_tag="P5179_07655"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRS95129.1"
                     /translation="MTGPGGTQWRPRKGLGQNFLVNEGIARRIVDAAGIAPHETVLEI
                     GPGMGALTRWLCEKAARVIAVELDPVLARELRLNGPASNLVVVEQDVLRTDLAALGRE
                     YGIDRWHIAGNLPYVIGSRLLMRLVEQARVVERATVMLQYEVAERILAPSGSRTYGLL
                     SVLLQATGRVRREFRVGAAAFRPAPKIQSLVFTWESTPAPDLDIPALIATTKAAFNQR
                     RKRLDNALKLLGVSADVIAEACRRAGIDPGNRAEQVSPSEFVRLSRACNELGVFPAEP
                     R"
     gene            7051..>7990
                     /locus_tag="P5179_07660"
     CDS             7051..>7990
                     /locus_tag="P5179_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95130.1"
                     /translation="MTRRILACAWWGAMAGAAAAAAPAFGTDFTLAFGKNLNVWSWNG
                     IFAHNRQLSSKWSAGTNASFDRKVSDSPGYLRRSTVVGGDVRTAYVVHPRLTLALTGK
                     ADLSRVQSGASISEVETNEILPTVLLTPVTGLSVKQGVGRAYDRRRNVSDAGLAYETG
                     VTFRPLQEPLGSRGWALVADYQHKGVSSRQGDVTKAFTLTTSYAREGWLNEDVRYSET
                     RDNQKYVSLLPANPILRRNVTGRSIRSSTMAVVPLLGSVQADAGYTRNSVEDEASNDP
                     NDLKYRTNNTTRGWMASANWTLPFSDPVLRSSISMGR"
BASE COUNT         1531 a         2477 c         2573 g         1409 t
ORIGIN      
        1 cgtggatgcc tactgcgggc ctgtcatgga ccgcctccac ccggcgctgc gaaagctgcg
       61 aagcccggca gtggacgccg tcctggcgga cttcgacaac tggcgcaatc ccgccgtgcg
      121 gaacggcatc atcccacggt tgctggcggc gatgggggag taaggtcgtc gagtgcagtg
      181 tgccgaattg cgcggcgcat tcgtcccata cgccccatag gacctattac ctgccagccg
      241 cacggtttgc tagccatttc ccgcgcacgc ctcctctgcc gtaccattcc ctgtcccggt
      301 tcgcaggtct ttcccctcac acggaggcgc acaatgagcg cagacgctat ccccgaccac
      361 atgaaatggt ggcgcgaagc cacgttcggc atgttcatcc actggggcgt gtatgccgtt
      421 cccgcgcgga acgagtgggt gatgtacgtg gaaaacatcc cgaaggagga gtacgcgcac
      481 tttgcggatg agtttactgc gcggcactac cacgcgcggg agtgggtgaa gctcgcgcag
      541 gacgccggca tgaagtacat ggtcctgacc acgcgccacc acgacggttt ctgcctgttc
      601 gacagcaagg tctccgactt caccgcgccg aaaaccgccg cgaaacgcga cctcatcgcc
      661 gaatacgccg atgcctgcca cgcgctgggt atgcgtatgg ggttctacta ctcgcttgag
      721 gactggcgct tcccccacca gttgccgcac ctgcccatgc gcgaggacaa aagcgtgtac
      781 caacccatgg tggagcaggc ccacgctcag gtgcgtgagc tcatgacgaa ctacggcacg
      841 gtggatatcc tgtggtacga cggtggcttc cccgccggaa tctggcgctc ggaggagctg
      901 aacgccatgg tgcggtcgct gcagccgaac atcgtcatca acgaccgctc aggtcttccg
      961 gaggatttcg gcactcccga gcagcacatc gccccgcaag accgcccgtg ggaggcctgc
     1021 atgaccatga acgacacgtg gggctacact gcgggcgata agaattacaa gtccgtgcgg
     1081 cagattctcg ggatgctgat ccagtgcgcg tcgcagggag ggaatctgct gctgaacgtc
     1141 ggcccggacg cggaggggcg gatccccgaa cccgcggtgg agcgcctgcg cgtggtcggg
     1201 gagtggatgc gcaccaacgg caaggcaatc tacggcgcaa cgcgggcccc gctcgtcacc
     1261 cacagcttcg ccacccccac ccgcgtcggg gacaggctgt acctcctctc cgctcactgg
     1321 cccggctcaa cggcgtcgtt cggctggtgc ggcaaccggg tgctctcggc gaagctgctg
     1381 gcgaccgggg aacggttgat cgtcgagcag aagggcgatc gcgtctggct ccgaggcctg
     1441 ccgcagtatg cgccggatgt gaatgtgtcg gtaatcgaga tcacggtgga gggcgagccg
     1501 aagtggccgg aggtgaggtt tacgtaacgg cgtgaggaac cacgaaccac acgaaccaca
     1561 cggaaaatcg ggaacgggaa cggctgcatg cccgaacgaa ccgatctcct cctccgggaa
     1621 gcggcttccc ggtcgtcgag tggcggccgc cgggtttccg gcggacgatg tatcgagacg
     1681 accggagcgt gccagtcggc cccgaagaga ttcttcactc cgttcagaat gacggtcggg
     1741 tcgggcgcca gcgagtcatt ctgagggaac ccgcgccgta tctgtcctat gctacccata
     1801 ggacctataa tatggcagac tcccgtaagg tccgccccga atggaggggc acgcttcgtc
     1861 gtgcccgcgc tgccagcgtg ctgcgaccgt cacttcgaca ggctcagtga ccgccttcct
     1921 cctgcccggt cacctgggtg gcggacatcc ccgttcccct tcctggattc ccgcccgcgc
     1981 gggaatgacc gaaccgcggg gcggtgattg gaaactgggc ggagcgctgt atccgggcga
     2041 ccggacgcat gagtggcatc cgcggcatat gcgtcctatg cgacccatag gacctgtact
     2101 ctgccgaact cccgccgtcc gcgcatggtg gggcaggcat tcatgcctgc cctgcttcct
     2161 ccaaagcgca cacggatcga agcggcagcc acaggggtct gcccttcccc tcccgtcatt
     2221 cccgcgcagg cgggaatcca ggcgacactt cccggcgccc tacccgttca ccaccatcgc
     2281 tttgatgacc cccggcttgc gggcggcaac atcatcgagg gcctgcttcg cgcggtcgag
     2341 ggggtattcc gcggtgatca tgctcttcac atccaccgcg ccgcggcgga tcatggagag
     2401 cgccgcgggg taggtgttgg cgtagcggaa cacgccccgg atgtctattt ccttgtcgat
     2461 gatggcgatg gtgtcgatcg ggatgatagc ggccggggaa aggccgatca gcaccactat
     2521 tccccccgcg cgaactatct gcacactttg cgccgtcgcc ttttccgagc ccgccgtttc
     2581 cacgacgatg tccggcccgc gcccgtgcgt aagatcgcgc agcttcgctg caagctctga
     2641 gtcgctggcg tcgaatgcct gagtcgcccc gagcgcaacg gcacggcggc accggtcggc
     2701 atcgagatca ctgacgtaca ccgctgctgc gcctcccgcg aggaacgcct gcagcgcgca
     2761 gaggccaatt ggcccgactc ctgcgataaa tacggtgtcc cccatgcgga gccccgacct
     2821 cctcacggcc tgcatccctg tggagagcgg ctcacacatg gcgccctcgt gcaggctcac
     2881 gccatcgagt ggaaacagga agtcggcaga atgggcgatg tactcggcaa aggccccgtc
     2941 cacgggtggc gtggccagga acaccacgtc cgggcagagg ttgtaccggc cctctctgca
     3001 gaaccggcac ctgcggcacg gcaccccggg ttccacggcc accagatctc ccggcctcac
     3061 gttccgcacc gcggcgccca cctccactac ctcgccggcg cactcgtgcc ccagcaggaa
     3121 cgggggctgg acaacgtacc ggccgatttt tccgtgcgtg taataatgga tgtccgaccc
     3181 gcacacgcca accgaccgta tgcggaccag cgcctcctcg tcgaggacgg ggggcaccgg
     3241 aatgtcttcg atgcgcacat cgcggattcc ccgcatgacc gcagctttca tgatgcctcc
     3301 ctggaggctg gcgcctcctt tgcgggaaag gactgcttcc ggttattgtc aacagaatgt
     3361 aatccgtcct ggcgccggca tgaagagagc gacgtatccg gtaggttccg ggggcagaaa
     3421 ccatttgcag gttccgcgcg gtagtattca caggacgcca gaaacgaaac aaccacctcg
     3481 aggaggtcgc acatgatcgt gcatcccggt attcccgaaa atctgcgctg gtttgacgaa
     3541 agccgtttcg gcatgttcat ccatttcggg ctttacgcgc tgctgggccg cggcgaatgg
     3601 gtgatgtatc acgaaaacat ctcccgcccg gaatatcgga agctgatgac cgtgttcaac
     3661 cccgagaagt tctccgcgga cgaatgggtc ggcctggcaa aagagggggg cgcgcgctac
     3721 atcaccgtga cggcgaagca tcacgatgga ttctgcctgt tcgactcatc cctgacggat
     3781 tacaagatca ccaacacgcc gttcggacgc gatctcatcg gcgaactggt ggatgcctgc
     3841 caccgcgccg atatgcggat cattttctac tactcacagc ccgactggta ccaccccaat
     3901 ttcgtgcaca accgcggcgc cttcaaggat ttgccgcacc cgccccccac cgacaaacct
     3961 gactgggaaa agtacgtgcg ctatttccac gggcaggtgc gggaactctg cacgaaatac
     4021 ggccggattg acggcatctg gttcgacggt tcgcacaaga cggtggagga gtggcgcggc
     4081 aaggagcttt acgacatgat caaggagcta cagccgaacg ccgtggtgaa tgaccgcgcg
     4141 cgctgggggg actttttcac tccggagcga agcctgccgg acgacctcac cggctacatg
     4201 ttcgaagcgt gcgagagcat cagcccgacg gcgtgggggt atcaggacga catgccggcc
     4261 cacacggtgc cgcatcttgt gcagagcctc gtccgcatgg cggcggcggg cggcaattac
     4321 cttctcaatg tcggcccgaa accggacggc actatcgccg aggaacaggc cgcggtgatg
     4381 cgtgagatcg gcaagtggct cacaaaaaac ggagagagca tctaccgcag tcacgccggg
     4441 ccaaggctgg aggacccgaa tattcgggtg actcgcaaag ggacgacgct ctatcttcac
     4501 ctgcttgaat ggccggcgcg gaacaggatt gtcctgccca acgtgatcgc ggagggagtg
     4561 aaggaggcgg cgctccttgg aagcggttcg gagctggagg tggtcgaaac cgacgaaggc
     4621 gcggagctgc gggggctgcc gatcatgccg ccggacccgc tggtgaacgt gatccgcgtg
     4681 gagttcttcc gtctgccgga gcacttcacg tttggcgcga aggaacaggc tgtcgctgtg
     4741 ccacaaccgg tttccgtgtc ggcgtcatcg ccgacgcatc ttacaccgga aacggcgagg
     4801 ttcgaaggcc tcggggtgaa aggagcgaaa ctgcgagtgc gcaggaacga agccggaaag
     4861 gcattcatct ccggctggat ggtgcccgac catgcggcac actgggacgc acgtgtcgag
     4921 gaatcagggc gttatcgtat tgacgtcgtt gttgccgcgc cggaaacaca tgcaggggcg
     4981 gtggtgaagc tgattgccgg atcaacggag ctgacgttca cggcgccgga gacgcgcagt
     5041 ctggacaccg tggaaaccgt gcacgcgggc acggtggagt tgtccgccgg ggtgacccgc
     5101 atcaccctgc agccggagaa actcaaatgg gggtatctct ctcccgacat cgcggaggtg
     5161 gtgctgacgc cggtggggtg actgcgcgga ccggggcaga gacggctcaa gcggcgaaga
     5221 gaagcgtccg ccgcgccgac tgtgaatgag gcatagcctt ggcgggtttc ccctgtcccc
     5281 tcaaaacaga catttttcag aaagggtaca gtctgaccgc agggatcaat gaggagcagg
     5341 ggataatggc gaagcagaca ggcatcggat gcgaacggtg tgaacgattc gggcggcttg
     5401 ggtaccgcct ggatacggat cgtggacagg ataacccgac cgggcgggtg gttgcctgtg
     5461 aatgtgcgcg ggagctttgc acgtgcggcg cgctccatga gccgacgttt gctccggcgc
     5521 ctccgtacga gcagcttccg gagcgggctg cggcggcgga agacgagact gcctgcatct
     5581 gctggcgcgt ccggcggcac gtgacaacac tgaaccggct cctgcgcgat gccggtatcc
     5641 ccaacctgta ccgcggcaag ctccgcgaag actaccacgt gcgctatccg gagggtcagg
     5701 tgattccgga cgccgaaagc gcgcgggggc aggcggtgac gtggctcgaa accgcggtga
     5761 aggagccgac ggggaaaagt ctgtttctgt acggggtgcc gggcgatggg aaaacgctgc
     5821 tggccagcgc gttcctgacg gagatcatcc tgcttacgcg caaatcaggg ctttttgtga
     5881 atctctcccg cggatatttc cagcgattgc gcaatacgta cgaagacgag ccctcaattg
     5941 aaacgacgga tcaggtgttc aaccgactca cccgggttcc gttcctggtt ctggacgata
     6001 ttggcgtaga acgggggagt gcgtgggaaa tcgaatggct gtacaacctg atagacgcgc
     6061 ggtatcagaa tcagaaccgt ctgatcgtga cgagcaacaa ctcgcttgag gagctcaacg
     6121 tgctctccca cgggcggatt tcctcccgtc tgcgccatgc gtgcatggtg gtgaggctac
     6181 ctgacgtcga ccttcgcgaa cggttccggt acggtgcgta atgacgggcc ccggcggaac
     6241 acaatggcgc ccgaggaagg ggcttgggca gaatttcctt gtgaacgagg ggattgcccg
     6301 ccgcatcgtt gatgcggcgg ggatagcgcc gcacgaaacc gtactggaga taggccccgg
     6361 aatgggtgcg ctcacacgat ggctgtgcga aaaagccgcg cgtgtgatag cggtggaact
     6421 cgacccggtt ctggcccggg agctgcgctt gaatggcccg gcttcgaacc tcgtcgttgt
     6481 ggagcaggat gtccttcgca ctgatctcgc ggcgcttggc cgtgagtatg gcattgaccg
     6541 gtggcacatt gccggcaacc ttccgtacgt catcggcagc cggctgttga tgaggctggt
     6601 ggaacaggcg cgcgttgtgg agcgggcaac cgtcatgctg cagtatgagg tggcggaacg
     6661 gatccttgcg ccttccgggt cgcgcaccta tggccttctg agcgtgcttc tgcaggcaac
     6721 gggacgcgtc cggcgggagt ttcgcgtcgg cgcagcggca tttcgacccg cacccaaaat
     6781 ccagtcgctc gttttcacct gggaaagcac tccggcgcca gacctcgaca ttcccgcgct
     6841 tatcgcgact acaaaggcgg cattcaacca gaggcgaaaa cggcttgaca acgcgctgaa
     6901 actgctgggt gtttccgcgg acgtcatcgc ggaagcctgt cggcgtgccg gcatcgatcc
     6961 gggcaaccgc gcggaacagg tgtccccgtc agaattcgtt cgcctcagcc gcgcgtgtaa
     7021 cgagcttggt gtgtttccgg cggaaccacg atgacaaggc gcattctcgc atgtgcgtgg
     7081 tggggggcca tggcgggcgc ggcggcagct gccgcgcccg cattcggaac ggatttcaca
     7141 ctggcgttcg gcaaaaacct caacgtgtgg tcgtggaacg gcattttcgc ccataaccgg
     7201 cagctttcct cgaaatggag cgccggcacc aacgcttcgt tcgacaggaa agtgtcggat
     7261 tcgccgggtt acctcaggcg atcaacggtg gtgggcgggg atgtccgtac ggcgtatgtg
     7321 gtccacccga ggcttactct ggcactgacc gggaaagccg atctgtcgcg cgtccaaagc
     7381 ggcgcgagca tctccgaagt cgaaaccaac gaaatcctgc ccaccgtcct tctcacaccg
     7441 gttaccggcc tttcggtaaa acagggggtg ggacgcgcgt atgaccgccg ccgcaacgtc
     7501 tccgacgccg ggctggcata cgaaaccgga gtcacgttcc gcccgctcca ggaaccgctc
     7561 ggcagccgcg ggtgggcgct tgtcgccgat taccagcata agggagtctc ttcccgccag
     7621 ggcgacgtta caaaggcgtt cacgctcacc acgtcgtacg ccagagaagg ctggctcaac
     7681 gaggacgttc gatacagcga gacgcgcgac aaccagaagt acgtaagcct ccttcctgcc
     7741 aacccgatcc tccgcaggaa tgttacgggc cggagcatcc ggtcttcaac gatggcagtg
     7801 gttccgttac tgggttccgt tcaggcggat gccgggtaca cccggaattc cgtggaggac
     7861 gaagcgagca acgacccgaa cgatttgaag tatcgcacca acaacacaac ccgcggctgg
     7921 atggcctcag ccaactggac gctcccgttc tctgatcccg tcctgcgctc ctcgatatcc
     7981 atgggaagga
//
LOCUS       DAOTNG010000132         7912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8138_length_7912_cov_5.402062, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000132 DAOTNG010000000
VERSION     DAOTNG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8138_length_7912_cov_5.402062"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="P5179_07665"
     CDS             complement(<1..1163)
                     /locus_tag="P5179_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HRS95131.1"
                     /translation="MATDSTEVRTKPARPEDLADVEEPFTDGFNWNTIMAMLFIGIVM
                     MPGAIYLGLVAGQGMGGAAEWVTIILFLEITKRSFITLKRQELILIYWSASMLVSVAG
                     SGLSGGIFAGLIWNQFFVQSPQAEAIAAYIPTWYAPPKGSAALLERTFMDAVWIQPVA
                     VVVISTILFQIVSLSLGYVIFRITSDIEHLPFPMAPVAAGGATALAETSTKSETWRWR
                     IFSIGTVIGLIWGTIYVVIPTLSGTFLTQAVAILPIPFLDFTVNLRSLLPTAVLAIST
                     DMSTLLAGFVFPYWTVIGMFIGSMVKNFVVNPMLYNYGILHRWEPGMSLIPTTIAVEF
                     DYWLSAKIGMAVAVFVISFYAVIRKLITGKDKISERPTDNLDPEKVGPGSGRG"
     gene            complement(1188..6422)
                     /locus_tag="P5179_07670"
     CDS             complement(1188..6422)
                     /locus_tag="P5179_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRS95132.1"
                     /translation="MAEVPQTSGDVPANGNGVNSSLWRLLGKIGKWLLIVFAVAQLLA
                     VTVFDPEKVSVWLFGLDLNPQRATRVDFGRVDAESQAVMDAVTRDNVARHMAAIVTGE
                     SRAVGYPGERKTFEYVRSEFQRLGLQDMGVDSFEVVVPRDEGGSVTFGGGETKPIYAL
                     WPNQVKTPTLPRAQISLNDTLLGPVSAVFPSKPSTFELPPEGVAAEFVTIPRHDLSLT
                     PEEQERFGARRGQVGIWDVVDFSRQQVRGKFVVFDLIGSAAKAKGSSETINIDSANVM
                     GARAAGARGVVFRDYQVTVLLNPERRLPELPDDILYFSADNQIAARLKDKNGNSSGVL
                     RFTPGIGGELLYAGSGDFHSFNGRKVEGSIVVMDFSTQKNYLNAHELGAQAIIFVDDG
                     KITREEAEAKFHLVPVDVARFWAEGACADTLRQLGSQPGNRVVLRSRMQWSPVRTYNI
                     WGRIPGTRTNRPEDTIVLEAYYDANSVVPARAMGAENAAGIAGLLAIAESFSQHPPEL
                     PILFLATSGHHSALSGINEWLYRHSRTENAFLQRIPQNQRIPFGLFIGLDLSSQNDQV
                     GSFAQGTFYTGWNTDARRTNMHTGYGKAFMRYAEELFPGQQVNEAYPQFVDGIAPAKR
                     TWRTFLPVAMAFDHEPVNYVGLSAITLATTNDIRERVDTPVDSLGAVNIDNVATQART
                     IAGLIHRGARDEGFFQETKIKPKDTQRELTGRILQFDRRKSFIPSTQIGGAIVTTSYG
                     NVKSFCGVRGMLATLTNSGPDDGLDCTRWTLTDTVATNQIPSQVGRFRFHMVGPHVPE
                     VMIKAYEIGDDGRIVMAPDLGQEGDKTYPMRVRADSPLLQTLQVLFEARALSLFEIID
                     SRYLSALDMMSVRGPDGSELRSYGMEVVPLQSRSEGKVESGAVAFAQPGTRVKVLMST
                     SLFGIKYLLINAPDEFFTNPLKPSEVTPAHQIRAEGSGYPVDLGRIDRTSWRVARDIW
                     VIDDVRMKTLTRFGIVNQRVMKLHEDAREALLQAKQAYDKQEYDGFIAMSRRAWGYEA
                     RAYPDVKGTAIDTIQGVIFYFALLLPFAFFMERLVIGAVDIRRQILGFGLIFVGIFFV
                     LQFVHPAFKLSTSPYIIFLGFVIFALGTIVTVIVVGKFNWELKRMKMAASGMHETDVG
                     RLQATAAAIALGINNLRKRPMRTGLTAVTIAILTFSVLSFTSVVTELKFYKIKRDNPP
                     AYDGMLVRDRNWFGLQASVLDYVQTAFRGKALITPRSWYMAQMKADKAFIDFRNPAND
                     KRSFASGILGVEADEAKVTALDKALVAGRWFAPGERGVCILPSRMAELAGVTAADVGT
                     AKLEMLGRVWTVIGLVDEQGISRRPDLDAEILTPVDLVAEQQRQTEASAEDPTQMAEA
                     PIQTFTHIAPENVLILPHVDVMEMGGMLRSVAVSNFAEPHEMLSQIEGFMSRVALTTF
                     VGHDNKVEVYSSLGATSLKGLSNLGVPIVVAALIVLNTMLGAVYDRKREIGIFATIGL
                     TPFHIAALFIAEACVFAVIGAVWGYLIGQVLAIALSQTSLLAGMSLNYSSLSAVASTL
                     IVMATVVLSSLYPAKVAADSAVPDVTRRWSFPPADGDYWRFDFPFTVGESDVIGLYAY
                     LQRVLEAYGAGSAGEFVAQNVELEALEVKEGEDPQYRLSFRTWLAPYDLGISQDVQMD
                     AVPTGEYRIYRIEMTIHRLSGDVDSWQRINRGFMNVMRKRFLVWRTIPPALKDEYRER
                     GKRIFRGETVGSTVNVDG"
     gene            complement(6726..7673)
                     /locus_tag="P5179_07675"
     CDS             complement(6726..7673)
                     /locus_tag="P5179_07675"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS95133.1"
                     /translation="MIRKDELEKRYPLPRGIISVLQTPFDDNRDVDTKSLERLVEHAI
                     GAGVNGFLVPAVASEVDTLSVKEREHVVEVVLKASGRRVPVIVGASAPSEEDCAAFAR
                     YAERVHADAYLAAVPSLLYAHQEKVQPFFAHVAASTSLPLVIQDLQFGGYGLRIEEIE
                     RLWETIPQLAGFKVETVPAGPKYSVIREKLGASVFVAGGWAIPQMIEALDRGVDALVP
                     ESSMVPVYRRVYELHESGRRSEAVTLFRRILPVLSYTNQELLTSIAFFKRLLCRKGIF
                     AREHMRLPGFQWDRFSERIAEELIALVLEVEATVSVRTG"
     gene            7699..>7912
                     /locus_tag="P5179_07680"
     CDS             7699..>7912
                     /locus_tag="P5179_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95134.1"
                     /translation="MNFKGNVVFWLGTLGAFAWGIEQVLAYTWFLSNVLYFSIPEGAF
                     RVVLALMVLCTCAAGFAALRNPEGIRI"
BASE COUNT         1693 a         2402 c         2174 g         1643 t
ORIGIN      
        1 tcgccacgtc cacttcccgg gcccactttc tccgggtcga ggttgtccgt cgggcgttcg
       61 ctgatcttgt ccttcccggt aatcagcttg cgaatcaccg catagaaact gatgacaaac
      121 actgccaccg ccatcccgat ttttgcggag agccagtaat caaactccac cgcgatggtc
      181 gtcggaatca gcgacattcc cggctcccat cgatgaagga ttccgtagtt gtacagcatc
      241 gggttcacaa cgaagttctt caccatggaa ccgatgaaca tcccgatcac ggtccagtac
      301 gggaatacga atccggcgag aagcgtgctc atgtccgtgc tgatggcaag cacggcggtc
      361 gggagaaggg aacgcaggtt caccgtgaaa tcaaggaagg ggatgggaag aatcgctact
      421 gcttgagtaa ggaaggttcc cgaaagggtt ggtatgacga cgtagatagt gccccagata
      481 aggccgataa cggtgccgat ggagaagatc cgccaacgcc acgtttcact tttcgtggat
      541 gtctccgcca gggcagttgc gccgcccgca gccaccggag ccatcgggaa gggcaagtgc
      601 tcgatgtcgc tcgtgatccg gaatatcacg tacccgagcg aaagcgacac aatttgaaac
      661 aggatcgtac ttatgacgac gaccgcgaca ggctgaatcc acaccgcatc catgaacgtg
      721 cgctcaagca gcgccgcact gcctttcggt ggtgcatacc atgtcggtat atacgccgcg
      781 atcgcttcag cctgcgggga ttggacgaag aactggttcc atatcagccc ggcaaaaata
      841 ccgccggaaa ggcctgatcc ggcaacgctg accagcatcg acgccgacca gtagataagg
      901 atcagttcct gccgtttcag ggtaatgaac gatctcttcg taatctccag gaaaaggatg
      961 atggtaaccc attccgccgc accacccatg ccctggcccg caaccagacc cagatatatg
     1021 gcaccgggca tcatgacgat accgatgaac agcatcgcca tgattgtgtt ccagttgaac
     1081 ccatcggtaa agggttcttc gacatccgcc aggtcttctg gccgcgcggg ttttgttcgt
     1141 acttccgtag aatccgtcgc cacgtctact ctcctgacgg gagcgtgtta gccatcgaca
     1201 ttgaccgtac ttcccacagt ctcacccctg aatatccgtt tgcctctctc gcggtactcg
     1261 tctttgagag ccggggggat agttctccac acgaggaagc gcttccgcat gacgttcatg
     1321 aaaccacggt tgatccgttg ccacgaatca acgtcgccac tcagtctgtg gatcgtcatt
     1381 tcgatccggt aaattcgata ctccccggtc ggcaccgcgt ccatctgaac gtcctgactg
     1441 atgccgaggt cgtacggcgc caaccaggtc cggaacgaca gccgatactg cgggtcctcg
     1501 ccttccttca cctcaagagc ctcaagttcc acgttctgcg caacgaactc ccctgctgat
     1561 ccggcgccgt atgcctccag aacccgctgc aggtacgcgt aaaggccgat gacgtcgctt
     1621 tcgcccacgg tgaacgggaa atcgaagcgc cagtaatcgc cgtccgccgg cgggaatgac
     1681 catcggcggg tgacatccgg cactgcgcta tcggcggcaa ctttggcggg atacagagag
     1741 gaaagtacaa cggtagccat cactatcagc gtggaagcaa ccgccgaaag ggaggagtaa
     1801 ttcagcgaca tgcccgcgag aaggctggtc tggctcagcg caatggcgag cacctgtccg
     1861 ataaggtacc cccacacggc cccaatcacc gcaaatacgc acgcttccgc gatgaacaga
     1921 gcggcaatgt ggaacggggt aagaccaatc gtcgcgaaga taccgatctc ccgcttcctg
     1981 tcgtacaccg cgcccagcat tgtgttaagg acaatcagcg ccgccactac gatcggtaca
     2041 cccaggttcg acaaaccttt gagcgatgtc gcaccaagac tgctgtacac ttccaccttg
     2101 ttgtcgtgcc cgacaaatgt ggtaagtgcc acgcgggaca tgaagccctc aatctgcgaa
     2161 agcatttcgt gcggttccgc aaaattgctc accgccacgg accgcagcat gccacccatt
     2221 tccatgacat ccacatgcgg cagaatcagg acattctctg gagcaatgtg cgtgaacgtc
     2281 tgtatgggtg cttccgccat ctgcgtcgga tcttccgcgg acgcttcggt ttgcctttgc
     2341 tgttctgcca caaggtcaac cggtgtcaaa atttccgcgt caaggtccgg gcgtctggag
     2401 atcccctgct cgtccacaag cccgataacc gtccacaccc ttccgagcat ctcgagtttc
     2461 gcggtgccga catcggcggc ggttactccg gccagctccg ccatccgaga gggaagaatg
     2521 cacacaccac gttcgcccgg cgcaaaccag cgtcccgcca caagtgcttt gtccagcgcg
     2581 gtaaccttcg cttcatctgc ctcaacaccg aggattcccg aggcaaaact ccgtttgtcg
     2641 tttgcgggat tacggaaatc aatgaacgcc ttgtcggcct tcatctgcgc catgtaccaa
     2701 ctgcgcggtg taatgagcgc tttgccgcga aacgcagtct ggacgtaatc gagaacgctc
     2761 gcctgcagtc cgaaccagtt gcggtccctc acgagcatcc cgtcatacgc agggggatta
     2821 tcccgcttga ttttgtagaa cttcagttcg gtcaccaccg aagtgaaaga aaggacgctg
     2881 aaggtcagaa tcgctatcgt aaccgcggta aggccggttc gcatagggcg tttccgcaga
     2941 ttgttgatac ccagcgctat ggcggcggca gtagcctgca gacggcccac gtccgtttca
     3001 tgcatgccgc tcgctgccat cttcatccgc ttcagttccc agttgaactt gcccacgacg
     3061 atgactgtca cgatggttcc aagcgcgaag atcacgaagc ccaggaaaat gatgtaaggt
     3121 gaagtactga gcttgaacgc ggggtggaca aactgcagca cgaaaaagat gccgacaaaa
     3181 atgagtccga acccgagtat ctgcctgcgt atgtctaccg caccgatgac aagtcgttcc
     3241 atgaaaaatg caaacggcag cagtaaggcg aaatagaaaa tcacaccttg aatcgtatct
     3301 atcgcggttc ctttgacgtc cgggtacgcc ctcgcttcat atccccatgc gcggcgagac
     3361 atcgcgatga aaccgtcgta ttcctgcttg tcgtacgcct gcttcgcctg caagagagct
     3421 tcccgggcgt cttcatgcag cttcatgacg cgctgattga cgatgccgaa tcgcgtcaag
     3481 gtcttcatcc gcacgtcgtc aataacccag atgtcgcgcg cgactcgcca cgacgtccga
     3541 tctattctgc cgaggtcaac cgggtacccc gagccttccg cgcggatctg gtgcgcaggt
     3601 gtcacctccg aaggcttcaa cggattcgtg aagaactcat ccggagcgtt gatcagcagg
     3661 tattttatgc cgaaaaggct cgtgctcatc aggactttca cgcgcgtccc cggctgcgcg
     3721 aacgcgacgg cgccactttc caccttgcct tcggagcggc tctgcaacgg gacaacttcc
     3781 atcccgtagc tgcgcagttc cgacccgtct ggcccacgaa cgctcatcat gtcgagtgca
     3841 ctcaagtacc tgctgtcgat tatctcgaaa agcgaaaggg cacgtgcttc gaacaaaacc
     3901 tgcagggtct gcaggagcgg gctgtctgcg cggacgcgca tggggtaggt cttgtcgcct
     3961 tcctggccga gatccggtgc catgacgatc cggccatcat cgccgatttc atacgccttg
     4021 atcattactt caggaacatg aggccccacc atgtggaacc ggaaacggcc cacctggctc
     4081 ggaatctggt tcgtggcgac tgtatcggtc agcgtccagc gcgtgcaatc cagcccgtcg
     4141 tcagggccgg aattcgtcag ggtggccagc atcccgcgaa caccgcagaa tgacttcacg
     4201 ttcccgtaac tcgtcgtgac tatcgctcca ccaatttgcg tgcttggaat gaaggacttc
     4261 cgccgatcga actggagaat cctgcccgtg agttcacgct gggtgtcttt cggcttgatc
     4321 ttcgtctcct ggaagaaccc ttcgtcgcgc gcgccgcgat ggatcagccc ggcgatagtg
     4381 cgggcctgag tcgccacatt gtcaatattt accgcgccca gggaatcaac cggcgtatct
     4441 actcgttccc gtatgtcatt ggtcgtcgcc aacgtgatcg cgctcaaacc cacgtaattg
     4501 acgggctcgt ggtcgaacgc catcgccacc ggcaggaacg tgcgccacgt tcgttttgcc
     4561 ggtgcgattc cgtccacgaa ctgcgggtac gcctcgttta cctgttgccc cgggaacagc
     4621 tcttcggcat accgcatgaa cgctttgccg tacccggtgt gcatattggt ccggcgcgca
     4681 tcggtgttcc atcccgtata gaacgtcccc tgcgcaaagg agccgacctg atcgttctgg
     4741 ctggaaaggt ccagcccgat aaacaggccg aacggtatgc gctggttttg cggtatgcgc
     4801 tggaggaatg cgttctcggt acgcgagtgg cggtatagcc attcattgat tccgctgaga
     4861 gcggagtgat gcccggaggt ggcgaggaaa agaatcggaa gctcaggagg atgctggctg
     4921 aaagactccg caatggccag cagaccggcg atccctgctg cattttcggc gcccatcgcg
     4981 cgagccggaa caaccgagtt ggcgtcgtaa tacgcctcca gaacaatcgt gtcttccggt
     5041 ctgttggtgc gggtcccggg aatccgaccc cagatgttgt aggtgcgaac gggcgaccac
     5101 tgcatccggc tacgcaagac cacccgattg ccgggctgac ttccgagttg gcgcagagtg
     5161 tccgcacatg cgccttccgc ccagaatctg gcaacatcca cgggaacaag gtggaacttg
     5221 gcttccgctt cttccctggt gatctttccg tcgtcaacga aaatgatggc ctgcgcaccc
     5281 agttcgtgtg cgttcagata gttcttctgc gtggaaaagt ccatcacgac gatggaacct
     5341 tcaacttttc tcccgttgaa ggagtggaag tcgccacttc cggcgtagag aagctccccg
     5401 cctatgcccg gcgtgaatct cagaacaccg gaggaattgc cattcttgtc tttcaggcgg
     5461 gcggcaatct ggttgtcagc agagaaatag agaatgtcgt caggcagttc gggaagccgc
     5521 cgttccgggt tgagaagcac cgtaacctga tagtcccgaa aaaccacgcc gcgagcgcct
     5581 gctgcgcgtg cccccatcac gttggcggag tcaatattga tcgtttcgga cgagcctttc
     5641 gctttcgccg cgctcccgat aagatcaaat acgacgaatt tgcctcgaac ctgctggcgc
     5701 gagaagtcaa ccacgtccca gattcccacc tgcccgcggc gtgccccgaa tcgttcctgt
     5761 tcctctggcg taagcgacag atcatgtcgg gggatcgtga caaactcagc ggcaacgcct
     5821 tccgggggaa gctcgaatgt ggagggtttg gaggggaaca ctgcggagac cggccccaga
     5881 agggtatcgt tcagagatat ctgcgcccgt ggcaaggtgg gcgttttcac ctggttcggc
     5941 cacagggcgt agatgggttt ggtttcgccg ccgccaaacg tgacgctgcc gccttcatcg
     6001 cgcggcacta ccacttcaaa cgagtctact cccatgtctt ggagtccgag gcgctggaac
     6061 tcgcttcgca cgtactcaaa tgttttccgt tcgcccgggt accccaccgc gcgcgattcc
     6121 ccggtcacga tcgccgccat gtggcgcgcc acgttgtccc tcgtgacggc atccatcaca
     6181 gcctggctct ccgcatctac gcgcccgaaa tccacgcggg ttgcgcgctg ggggttcaag
     6241 tccagcccga acaaccagac ggatactttt tcggggtcaa acaccgtgac ggcaagcagt
     6301 tgcgccacgg caaacacgat gaggagccat ttgccgatct tgccaagcaa tcgccacaaa
     6361 gaggaattca cgccattccc gttggcgggg acgtcgccag atgtttgggg aacctcagcc
     6421 attggcatct ccgtacggta ttctcaaaac gtcgaaagct cttgctccag gtcttgcggc
     6481 aattttccgg gcgtagactc gggcctggaa tccgttgtgt acggtgcagc aggcaatacc
     6541 tgtcgattca gtagtggggc ctgaagtcag gcctgtggcc ctctgctctc tcaagaaaac
     6601 ggtgggaagc tcacatgaga tcgcgaaaaa cgccggttga acatattcca tcgagaactc
     6661 tggcacgcgt tccaatggaa gtatacacac gaacgcggag gcggccaacc cgtgcttttc
     6721 cggaatcagc cggtgcgcac agagaccgtg gcctccactt ccagaacaag ggcaatcagt
     6781 tcctccgcaa tccgctccga gaacctgtcc cattggaaac cgggaagacg catatgttca
     6841 cgcgcgaaaa tgcctttgcg acagagaagt cgtttgaaga aggcgatgga ggtcagaagt
     6901 tcctgatttg tgtacgagag caccgggaga atccgtcgaa acaacgttac tgcttcgctt
     6961 ctgcgtccgc tttcatgaag ctcgtacacc ctccggtaca cgggcaccat cgaactctcc
     7021 ggcaccagag cgtctacgcc gcggtcgagc gcttcgatca tctgcggaat ggcccatcct
     7081 cccgcaacaa agacgctcgc acccagtttc tcccgtatca cgctgtactt cggacccgcc
     7141 ggaacggttt ccaccttgaa cccggcaagc tgaggaatgg tctcccacag gcgttctatc
     7201 tcctcgatcc gcagaccgta cccaccgaac tggaggtcct gaatcaccaa ggggagggaa
     7261 gtggaggcgg caacatgggc gaaaaagggt tgaaccttct cctgatgggc gtaaaggaga
     7321 gacggaaccg ccgcaaggta cgcgtccgcg tggacccgct ccgcgtaccg cgcaaacgcg
     7381 gcgcaatcct cctccgacgg tgccgatgct ccgacgatta ccggtaccct ccggcctgaa
     7441 gctttcagaa caacctctac gacatgttcc cgttccttca cggaaagggt atccacttcg
     7501 ctcgcaaccg cgggtaccag aaacccgttc acacccgccc cgatagcgtg ttccaccagg
     7561 cgctccagcg atttcgtgtc tacgtcccgg ttgtcatcga agggagtctg aagtacgctg
     7621 attattccac gaggcaacgg gtaacgtttt tccaattcat ctttcctgat catgtcttca
     7681 tccggcgcgg gaggtcacgt gaacttcaaa ggcaatgtcg ttttctggtt aggcaccctc
     7741 ggggcattcg cgtggggaat cgaacaagtg ctggcgtaca cctggttcct ctcaaatgtc
     7801 ctctacttct cgattccgga aggtgccttt cgagttgtac tggctttgat ggtgctgtgc
     7861 acgtgcgcgg ccggattcgc ggcgttgcgc aatcccgaag gtatacgaat cc
//
LOCUS       DAOTNG010000133         7883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8168_length_7883_cov_5.496679, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000133 DAOTNG010000000
VERSION     DAOTNG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8168_length_7883_cov_5.496679"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2885)
                     /locus_tag="P5179_07685"
     CDS             complement(81..2885)
                     /locus_tag="P5179_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS95135.1"
                     /translation="MPTGYEMHVISNTHWDREWLYNFQDTRMFLVEFMDKLLNILDKE
                     PDYAAYLMDSQVIPIEDYLEVRPEARERVTKHIKNDRIMVGPWYTLPEEFNVSGESLV
                     RNLLWGVKKSQAYGKVTLTGYSPFSYGQTSQMPQIYRGFDIDTILFYHGIQPEETKSE
                     FIHEAPDGSRVYGSRMGSMARYNFYFGVWRPAVYGMKTPDRKYQWSKGWLPFHLAGPH
                     RYQGHHFLLDVKKEINEANLRQALADLKAEELKHARTNYLAYMQGLDSTEPDLLEVEL
                     IRKAKHLLEPGDTILHSSLPAYLAKVKEAVKDMDLTVLVGERRTPRALGQKVHLYGDV
                     TSSRSRMKQANTRTEYALQRLAEPFCVAAATFGAEYPQTLLDLAWKYLLQSHPHDSIA
                     GSGVDQIELDVLNRLDQARNIAEGVMRRALGAMQMRINNSSVAWDEIVLTVFNPSPYE
                     RDETVTAFVDLPDEVKIEYYTLHEATTGRRVDHQEVSRARQMAVARHLGDATMEMNSD
                     QIKLHFWAEKIPALGYRTYILKRHDEPTGTIGTLLAGARTLENEHLRVEVACDGTLTV
                     TEKATGRVYTGLNEFEDSGEAGHPWRHVAPAYDEVISSIGAPFTVSVVEAGPLSATLR
                     IEHRMQIPVKMEEDQSDTIRRLDANGDGARRSAERKEMVITSLVRLVRGAKSVEVTTS
                     FDNECKDHRLRVCFPTFINAKKSAAETPFDVVEREIDRPEGSPWALSPNPTHPMGRFV
                     DVSDGVNGLAVINDGLREYEVVDTKERRLCITLMRAYQVELTTVSWRWERHPEMELAQ
                     CPGKHTFTYRIYPHSGTWADGKTFLEVEKLNLPVEVAQACSHPGDLPQELSFFSIAPN
                     DLVLSGMKTAEDGNGIVLRVVNPTTRDQKVTVTAWKPIAAAELVQLNEKPVEKLSPAG
                     SSVSFAAGPKKIMTVRVVLA"
     gene            3113..3442
                     /locus_tag="P5179_07690"
     CDS             3113..3442
                     /locus_tag="P5179_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRS95136.1"
                     /translation="MPYTQYMEFIEATAFSRHVHEYLSDTEYLGLQLHLMRYPEAGRV
                     VPGSNGVRKLRWSMAGRGKSGGVRVIYYFKKQDDEIWLLTIYDKRETENIPAHTLRQI
                     AKEIDDV"
     gene            3435..3722
                     /locus_tag="P5179_07695"
     CDS             3435..3722
                     /locus_tag="P5179_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS95137.1"
                     /translation="MSERQIGQEILDGIKEIKAHKAGKKALHTHVLSEPSPPHAIRAK
                     LKLSQSAFARSMGVSLRTVQEWEQGRRKPSGPATALLRIAEQKPEVFKALT"
     gene            complement(3706..5268)
                     /locus_tag="P5179_07700"
     CDS             complement(3706..5268)
                     /locus_tag="P5179_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRS95138.1"
                     /translation="MRLVTARQMAAIDRRTIDHHGVPGIELMHRAGQAVVEELLARTG
                     SVAGDRFVVLCGKGNNGGDGFVIARLLRENNAAVAVYLLGERERVTGDARSAMEEWRA
                     AGGEVATLTEETAVHEAAASWRDADYLVDALLGTGLSGEVQGLMRAAIEEVNRLRVPV
                     IAVDIPSGVAGDTGLVCGVAVNAMLTVTFGLPKVGQALYPGRRHCGTLSVADIGFPEA
                     AVREALEGEPPIFMTSKADAARWMPKRQPDAHKGDAGRVMVVAGSVGMTGAAALASEA
                     ALRAGAGLVYLGCPESLNDIVETKLTEVITLPLPEIRRRRCLALRAVGDIRRLLPQMQ
                     VLAIGPGIGRFRETAELVRRVVSESATPMVIDADGLFALAGAGLFPLPQPAVLTPHYG
                     EFARMVGCEIADIQRDPVESCRRAAGEWEVTLLLKGAPTVVCGPSGETWVNPTGNAGM
                     ATAGAGDVLTGAIAGLIAQGVAPEVAARLGAFAHGMAGDFARDELGTHGMIAGDILRH
                     LPKAMRELTSAP"
     gene            complement(5358..6065)
                     /locus_tag="P5179_07705"
     CDS             complement(5358..6065)
                     /locus_tag="P5179_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRS95139.1"
                     /translation="MIRAVIFDLDNTLVDFMAMKKQAVEAAAWAMVDSGLPMTIEEAV
                     AGVYRTYDERGIENQQVFDHFLTETLGQIDYKILAAGIVAYRKAREAALQPYPHVKMT
                     LTQLLRNGLKLAVLSDAPRREAWLRLYQLGLHHTFDVVVTFEDTGLRKPAKEPFERVL
                     SLLDVAPKEAVMVGDWPSRDVTGAKAVGLWTVWAQYGDTFGTHSTDADYEIATIRDIG
                     RVIESINARMGGSEQTR"
     gene            6643..7722
                     /locus_tag="P5179_07710"
     CDS             6643..7722
                     /locus_tag="P5179_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95140.1"
                     /translation="MKRTMTSKERVLTTLAHAEPDRVPINYHANAGIDRRLKEHYGLD
                     PKDGEGLAKVLGVDFRGVGAPYVGPKLHPDLPDRHVDMWGIHTRWIEHESGGYWDYCD
                     FPLKDADEEMIRAWPMPSPDDFDYSHVEEECKKKEHFAIHCGGAGLGDIINSSGMIRT
                     MEQVLVDLMTDDPAGLALIDRRLGILFEVTRRTIEAAKGRIDLMWMGEDLGTQYTPLI
                     SLELYRKHLRPRHQRFVDLAKHYNLPVIIHTCGSSSWVYEDFIEMGFTGVDTLQPEAA
                     NMSPEYLKRTFGGRLAFHGCISTAGPVAYGTVEEVVEDVRRTLEIMMPGGGYCLSPTH
                     ALQDNSPTENVVAMYDAAHKYGVYG"
BASE COUNT         1432 a         2559 c         2423 g         1469 t
ORIGIN      
        1 accgtggagg acccaaacag ctacgcaaac ttgacaacca agacagttac tacccccaca
       61 aattgtgttc aatgcccgtt ctacgccaat accacccgca cggtcatgat cttcttcggg
      121 ccggcggcga acgacacgct tgaaccggcg ggggagagct tctccaccgg tttctcgttc
      181 agttgcacca attccgccgc ggcgatgggc ttccacgcgg tcacggtcac cttctgatcc
      241 cgcgtcgtcg gattgacgac gcgcagcacg atgccgttgc cgtcttccgc cgtcttcatg
      301 ccgctcagga cgaggtcgtt cggcgcgatg ctgaagaagg agagctcctg cggcagatcg
      361 ccgggatggc tgcacgcctg cgcgacttcc accggcaggt tgagcttctc cacttcgagg
      421 aacgtcttgc cgtccgccca cgtgccgctg tgcgggtaga tgcggtaggt gaacgtgtgc
      481 ttgccggggc actgggccag ctccatttcc gggtgacgtt cccagcgcca cgacacggtg
      541 gtcaactcca cctgatacgc acgcatcagc gtaatgcaca ggcggcgttc cttcgtatcc
      601 accacttcgt attcccgcag gccgtcgttg atcaccgcga ggccgttcac tccgtcggag
      661 acatccacga accggcccat cggatgcgtc ggattgggcg aaagcgccca cgggctgcct
      721 tcgggacgat ctatttcgcg ttccaccacg tcgaacggcg tctccgcggc ggatttcttc
      781 gcgttgatga atgtcgggaa gcagacgcgc aggcggtggt ccttgcactc gttgtcaaac
      841 gacgtggtga cctcgaccga ttttgccccg cgcaccagcc gcacgagcga agtgatcacc
      901 atctccttcc gctcggcgga gcgccgcgcc ccgtccccgt tggcatcaag ccggcggatg
      961 gtatccgact ggtcctcttc catcttcacc ggaatctgca tccggtgctc gatcctgagc
     1021 gttgcgctga gcgggcccgc ctccaccacc gacacggtga acggggcgcc gatggaggaa
     1081 atcacctcgt cgtacgccgg agcgacgtgg cgccaggggt gacccgcttc gcccgagtcc
     1141 tcgaactcgt tcaggcctgt gtagacgcgg ccggtggcct tctcggtgac cgtcagcgtg
     1201 ccgtcgcagg caacttcgac gcgcaggtgc tcgttctcca gcgtacgtgc gccggcaagc
     1261 agggttccga tggtgccggt gggctcatcg tgccgcttga ggatgtacgt cctgtatccc
     1321 agcgcgggaa tcttttccgc ccagaaatgc agcttgatct ggtcgctgtt catctccatg
     1381 gtggcgtcgc cgaggtgacg tgcgacggcc atctgccggg cgcggctgac ctcctgatgg
     1441 tccacccgcc tgccggtggt tgcttcgtgg agggtgtaat actcgatttt gacttcgtcc
     1501 ggcaggtcca cgaaggcggt gacggtttcg tcgcgttcgt agggggaggg attgaacacc
     1561 gtgagcacga tctcatccca ggcgacggac gagttgttga ttcgcatctg catggcgccg
     1621 agcgcacgcc gcatgacgcc ttccgcgatg ttgcgcgcct gatcgaggcg gttgagcacg
     1681 tccagctcaa tctggtccac gccgctgccc gcgatggaat cgtgcgggtg gctctgcagg
     1741 aggtacttcc acgccagatc gagcagcgtc tgcgggtact cggcgccgaa tgtggcggcc
     1801 gccacgcaga acggctcggc gaggcgctgg agggcgtatt ccgtgcgcgt gttggcctgt
     1861 ttcatgcggc tgcggctgct cgtcacgtcg ccgtacaggt ggactttctg ccccagtgcg
     1921 cgcggggtcc gccgttcgcc cacgagcacc gtgaggtcca tatccttcac cgcttccttc
     1981 accttggcga ggtatgcggg caggctgctg tggagaatgg tgtcgccggg ttcgagcagg
     2041 tgtttcgcct tgcggatgag ctcgacttcc agcaggtcgg gctcggtgct gtccagcccc
     2101 tgcatgtagg caaggtagtt cgtccgcgcg tgcttcaatt cctccgcctt gaggtcggca
     2161 agcgcctgcc gcaggttggc ctcgttgatc tccttcttca cgtcgagcag gaagtgatgc
     2221 ccctgatacc ggtgcggtcc cgcaaggtgg aacggcagcc agcctttgga ccactggtac
     2281 ttccggtcag gggttttcat tccgtagacc gccgggcgcc agacgccgaa gtagaagttg
     2341 taccgcgcca tcgagcccat gcgggagcca tacacgcgcg agccgtccgg cgcctcgtgg
     2401 atgaactccg atttcgtttc ctccggctgg atgccgtggt agaacagaat cgtgtcaatg
     2461 tcgaacccgc ggtaaatctg cggcatctgg gaggtctggc cgtaggagaa cgggctgtac
     2521 ccggtcagcg tcactttgcc gtaggcctgc gatttcttca cgccccagag caggtttcgc
     2581 acgagtgact cgccgctgac gttgaactcc tccggcagcg tataccacgg gccgaccatg
     2641 atgcggtcgt tcttgatgtg cttcgtcacg cgctctcgcg cttcggggcg cacttcaagg
     2701 tagtcctcga tcggaatcac ctgggagtcc atcaggtacg cggcgtagtc cggctctttg
     2761 tccaggatgt tcagcagctt gtccatgaat tcgacaagga acatgcgggt atcctggaag
     2821 ttgtaaagcc attccctgtc ccagtgcgtg ttgctgatca catgcatctc gtagccggtc
     2881 ggcatggcga gggtcctttc gtggaagagg tcggtgcggg agtaatcaga cagcgttgtg
     2941 caacggtgaa aaggtacagc ggggggaggg aagtggcaaa cggagcgggg gggaggagcg
     3001 ccggcaaaga aggtgagacg gatggcgtgt gcgtcgcggc ccttcggata catcatgacc
     3061 ttccacccgg gctcttgtct gaaggacatt tggagttgcc aggcatacgg ctttgccgta
     3121 tactcaatac atggaattca tcgaggcaac agccttctcc cgccacgtgc acgagtacct
     3181 ttcggatacc gagtacctgg gcctgcaact gcacctgatg aggtaccccg aggcagggag
     3241 ggtggttccg ggctcgaacg gcgtgagaaa gctccgctgg tcaatggcag gcagaggaaa
     3301 aagcggtggc gtgcgcgtga tctactattt caaaaagcag gacgacgaaa tctggttgtt
     3361 gaccatttac gacaaacgag agacggaaaa catacccgct cacactctga gacagatcgc
     3421 aaaggaaatc gacgatgtct gaacggcaga tcggtcaaga gattcttgac ggcatcaagg
     3481 agatcaaagc gcacaaggcg gggaaaaagg ctctgcacac ccatgtgctg tccgaaccgt
     3541 ccccgcctca cgccatccgc gcaaagctga aactttcgca atcggccttc gcacgaagca
     3601 tgggggtcag cctgcgcaca gtgcaggaat gggagcaggg ccgccgcaag cccagcgggc
     3661 ccgccacggc gctgttgagg atcgccgagc agaaacccga agtcttcaag gcgctgacgt
     3721 aagctccctc atcgccttcg gaaggtgccg caggatgtcg ccggcaatca ttccgtgggt
     3781 gccgagttcg tcccgcgcga aatcccccgc catgccgtgt gcaaacgctc cgagccgcgc
     3841 ggcaacctcg ggtgccacgc cctgcgcaat caagccggca attgcgccgg taagcacatc
     3901 accagcgcct gcggtggcca ttcccgcgtt gccagtgggg ttcacccagg tctcgccgga
     3961 tggcccgcac accaccgtcg gcgcaccctt gaggagcagc gtcacctccc actcgccggc
     4021 ggcccggcgg cacgactcca ccgggtcgcg ctgaatgtcg gctatctcgc agcccaccat
     4081 ccgcgcgaac tcgccgtagt gcggcgtcag gacggcaggc tgaggcaacg gaaacaggcc
     4141 ggcaccggca agcgcgaaca gcccgtccgc gtcaatcacc atcggcgtgg ctgattccga
     4201 aacaacgcgg cggacaagct ccgccgtctc cctgaaacgc ccgatccccg gacctatcgc
     4261 cagcacctgc atctgtggca gcaatcggcg gatatccccc accgcgcgga gtgcgagaca
     4321 tcgccgcctg cgtatctccg gcagcggcag ggtgatcact tccgtcagct tcgtctcgac
     4381 gatgtcgttc aggctctccg ggcaaccgag gtagacaagc ccggcacccg cgcgcagcgc
     4441 cgcttccgag gccagcgccg cggcgcccgt catcccgacg gatcccgcca ccaccatcac
     4501 acggccggca tcgcccttgt gcgcgtccgg ttggcgcttc ggcatccacc gcgcggcgtc
     4561 ggccttcgac gtcatgaaga tcggcggctc gccttccagc gcctcgcgca ccgccgcctc
     4621 cgggaatccg atatccgcca cggagagcgt tccgcagtgc cgccggccgg ggtacaacgc
     4681 ctgccccacc ttgggcagcc cgaacgttac cgtcagcatc gcattcaccg caacgccgca
     4741 gaccagaccc gtgtcgcccg ccacccccga cgggatatcc acggcaatca ccggcacgcg
     4801 cagccggttc acctcctcga tggccgcccg catcaggccc tgcacctcgc cggagagccc
     4861 ggtaccgagc aacgcgtcca cgaggtaatc cgcatctctc caggacgccg cggcttcatg
     4921 caccgccgtt tcttccgtca acgtggcaac ctcaccgccg gcggcacgcc actcctccat
     4981 cgccgaccgt gcatcacccg ttacgcgctc tcgttccccc agcaggtaca ccgccacagc
     5041 ggcgttgttc tccctcagca gccgcgcgat gacgaatccg tcgccgccgt tgtttccctt
     5101 gccgcacagc acgacgaacc gatctcccgc cacggacccc gtccgcgcga gcagttcctc
     5161 taccaccgcc tgcccggcgc ggtgcatgag ctcgataccg ggtaccccgt ggtggtcaat
     5221 cgtccggcgg tcaatcgccg ccatctgccg tgccgttacc agccgcatgg gtagttcctc
     5281 tgagtcgaga cggggaaacc ggtcactgag gctgtcgaag tgccggtgtg cttcgatagg
     5341 ctcagcaacc ggctatttca ccgggtttgt tcgctcccgc ccattcgcgc atttattgac
     5401 tcgattaccc gcccgatgtc gcgtatcgtc gcgatttcgt agtcggcatc ggtggagtgc
     5461 gtgccgaagg tgtctccgta ctgtgcccag acggtccaca ggccgaccgc cttcgctccg
     5521 gtcacgtccc gcgaaggcca gtctcccacc atgacggcct ccttcggtgc cacatccaga
     5581 agcgaaagta cgcgctcaaa tggctccttt gccggcttgc gcagacccgt atcctcgaac
     5641 gttaccacca cgtcgaacgt gtggtgcagg ccgagttggt acaaccgcaa ccatgcctcc
     5701 cgccgcggtg catccgagag caccgccagc ttcaggccgt tccgcaggag ttgcgtcagc
     5761 gtcatcttca cgtgcggata cggctggagc gccgcttccc gcgctttccg gtaggccaca
     5821 atccccgccg caaggatctt gtagtctatc tgcccgagcg tttcggtgag gaagtggtcg
     5881 aacacctgct ggttctcgat cccgcgctcg tcataggtgc ggtagacccc tgccacggcc
     5941 tcctcgatcg tcatcggcag gcccgaatcc accattgccc acgccgctgc ttccacggcc
     6001 tgcttcttca tggccatgaa atccaccagc gtgttgtcga ggtcgaatat gacggcgcgg
     6061 atcatgaact ggtctccctg acgggatact ggtccccctt gcaggatacg ggtctccaga
     6121 gctctcaaac gcgcggcgag cacgtgttgg aaaagtagca cctgccagcg ctgaggacaa
     6181 cgttatccgg ggtccccgat ttgcggcgag cagagcacca gtgcgtgaac cggccgggta
     6241 cggcgaagcg tgcgcgccga cgcgttgttg ccttccatcc ttgaccgcgc gttcaagggg
     6301 taattgtaga gggtgggcac aggagcccgc ccctgcaaaa aagcttttcg tgcatttcgt
     6361 gtggttcgtg gtaaaaaaca cgggccgaca cgcaggtcgg cccctacggc ttcctctttc
     6421 gcgttcttcg cgcgcgttct tgttgctccc aaaaatcccg taccccgatt gaaccatttt
     6481 cgcagaaacc cttgacacga acacgccgca tcgcctgtgt catacagtga cctcagcgac
     6541 agggcagcgc gccgtcagaa cgccgtttgt ccgccagccg gggcgtggta cgttctaccg
     6601 tcccgaaagg gtgaaacgcc gattcgacaa ggggaacccg ccatgaagcg cacgatgacg
     6661 tccaaagaac gcgtgttgac gacactcgcc cacgcggagc ccgaccgcgt gccgatcaac
     6721 taccacgcca acgcggggat agaccgccgc ctgaaggagc actacggcct cgatccgaaa
     6781 gacggtgagg ggctcgcgaa ggtgctgggg gtggatttcc gcggcgtggg cgcgccgtac
     6841 gtcgggccga agctccaccc cgacctgccc gaccgccacg tggatatgtg gggcatccac
     6901 acccgctgga tcgagcacga aagcggcggc tactgggatt actgtgactt cccgctgaaa
     6961 gacgccgacg aggagatgat ccgcgcgtgg cccatgccgt cgcctgacga cttcgattac
     7021 tcgcacgtgg aagaggagtg caaaaagaag gagcatttcg caatccactg cggcggcgcg
     7081 gggctggggg acatcatcaa cagcagcgga atgatccgca ccatggaaca ggtgctggtg
     7141 gatctgatga cggacgaccc ggcgggtctc gcgctgatcg accgcaggct gggcatcctt
     7201 ttcgaagtga cccggcgcac catcgaagcg gcgaaggggc gcatcgacct gatgtggatg
     7261 ggagaggacc tcggcacgca gtataccccg ctcatcagcc tggaactcta ccggaagcac
     7321 ctgcggcccc ggcatcagcg cttcgtcgat ctggcaaaac actacaatct gccggtgatc
     7381 attcacacct gcggctccag cagctgggtg tacgaggact tcatcgagat gggcttcacc
     7441 ggagtggata cgctccagcc ggaagcggcg aacatgtccc cggagtacct gaagcgcaca
     7501 tttggcgggc ggctggcgtt ccacgggtgt atctccaccg ccggcccggt agcctacggc
     7561 acggtggagg aggtggtgga ggatgtccgc cgtacgctgg agatcatgat gccgggcggg
     7621 ggatactgcc tctcccctac gcacgcgctg caggataact ccccgacgga gaacgtggtg
     7681 gcgatgtacg atgcggcgca caaatacggt gtgtacgggt agtccgaagg tctcacacga
     7741 agccgcgaag atcgcgaagg agtgttgagg ccttttgaag aaagtcttac ttcgtgtctt
     7801 cgtgccttcg tgtcttcgtg cgagcagaaa aaggttctca cacgaagccg cgaagaacgc
     7861 gaagaggagg gggattctgt gag
//
LOCUS       DAOTNG010000134         7871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8177_length_7871_cov_5.565763, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000134 DAOTNG010000000
VERSION     DAOTNG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_7871_cov_5.565763"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..573)
                     /gene="efp"
                     /locus_tag="P5179_07715"
     CDS             complement(16..573)
                     /gene="efp"
                     /locus_tag="P5179_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRS95141.1"
                     /translation="MATTQDFRNGFAFRMDGNIYFIVEFQHVKPGKGGAFVRTRLKNV
                     QTGAVIDRTFKSGEAVEEIRVENRDMQYIYKEGDHYVFMDTETYEQIHLPHEIVDDIA
                     DLLKENEVVTVIIGDDMPLLVELPNHVTLTIADTDPGVRGDTATGGTKPAKLETGAVV
                     QVPLFLGVGEKIRIDTRTRQYLSRA"
     gene            complement(628..1830)
                     /locus_tag="P5179_07720"
     CDS             complement(628..1830)
                     /locus_tag="P5179_07720"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRS95142.1"
                     /translation="MVFFLTAIPVAVPVYSLPSAGRFRFPGACRNFPSGEVTLPQPRI
                     VALRRAMRAERVDRFLVTCPEHVRWLSGFSGSSAWLVVGAREKVLLTDFRYRDQAAVQ
                     APDWRAVITQNGLVACVRELAAGWGDSRVGFESAHLTVKDHRALTSPFKDGDTPLSVQ
                     WVATEQIIERELFVKDEAEIAAIARSAAIADAAFAEVLPLVKPGVTERDLATELEYRV
                     RKLGADRMAFPPIVASGPNAALPHAQPSDRRIRNGDMVTFDIGAQRDGYASDMTRTVA
                     VGKASKKLQKVYGIVLEAQMRAVAAVRPGLSCVELDSVARSYIQEQGFGEAFGHSLGH
                     GVGLRVHDGPSISWRSQETLAEGMVITIEPGIYLTGWGGVRIEDLAVVTGKGCRVLCS
                     TPKELRIV"
     gene            1944..2783
                     /locus_tag="P5179_07725"
     CDS             1944..2783
                     /locus_tag="P5179_07725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS95143.1"
                     /translation="MGIRTAIQGLRSAMRVIAHAGRAVWLFFSERRPAPKPDHPLVVK
                     VVYPGGAATRDFSFPLRTVWKAKIAVAVVFVLFFVGLAAIGDAAYSRWRLYHLNAQLR
                     EAQTNEQLTLEMRRQLAEIWLINERLQRMLGRHESYRTRQTVVYRLPQGLPVSRWVGV
                     AFGVPKALPEDMRACYLCAPGSLVLATADGKVTDLSWDPRNGDVVTIDYGEGVTARYG
                     RDALFFVEKDELVRKAQPIGVIHAPDSLSTPLLFYQVTAEGEEVETVPYMVVGGTTGI
                     YTE"
     gene            2818..3195
                     /locus_tag="P5179_07730"
     CDS             2818..3195
                     /locus_tag="P5179_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRS95144.1"
                     /translation="MAKFQAVTSATINTIIGENSRVVGDFDIAGSIKVEGFLKGKLTT
                     SERLVIGLGGVVLADANVRDAIIGGTFTGTLYAETRVEMESTAKVRGDVRTKQLVIHE
                     GAEYEGNISRGEEPSAEAEGDTG"
     gene            3409..4164
                     /locus_tag="P5179_07735"
     CDS             3409..4164
                     /locus_tag="P5179_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95145.1"
                     /translation="MNYQRHAWGWALAAACAVGLLNAPAHAIAGFGIHAGKDFVSISE
                     GSFDRAKFEAAGTRLGLTGVNWANWNSVTLTRKEISSPWLIGVHAYIDAIPYIDLEVS
                     VDGAISQYRVDYRSTLNPAVNESKDVYFGRVGAYGTVRRDIVKFPPVVPIAALYFGGG
                     LSYNFIAPVAGPDLIVNAYGTDNPTTRQPDIAKLVEREGTFGWHGLVGFRIKPPVVPI
                     ALRLEGKYTSTGISTYERPKSIFSAYLGTSFAF"
     gene            4246..4440
                     /locus_tag="P5179_07740"
     CDS             4246..4440
                     /locus_tag="P5179_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HRS95146.1"
                     /translation="MVSEGLTRESEITCPQCGFNKVETMQIDACQYFYKCEGCGVVLR
                     PKSGDCCVFCSYGSHPCPPV"
     gene            4672..6033
                     /locus_tag="P5179_07745"
     CDS             4672..6033
                     /locus_tag="P5179_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRS95147.1"
                     /translation="MSEDEELIETDLFDGVEKPDTTAPLADRIRPRTLAEFAGQRHII
                     GEGALLRRMVEQDRLVSLLFWGPPGTGKTTLAFVLARMLNARFVTMSAVTSGVKDVKA
                     VIERAAMEKRRGRRTILFIDEIHRFNKAQQDALLPHVERGTVIFIGATTENPSFDVNS
                     ALLSRCRVFVLKQLLPEDIEQIVRRALADRDRGLGGLELVMDDDAVDALAKLADGDAR
                     IALNALEAAANVVSAGDRHITKQVIIEALQQRTYDYDRAGEGHYNLISALHKSVRSSD
                     VNAALYWLARMLDAGEDPRYLARRMVRMAVEDIGLTDPRALSVALAAHETYHMLGSPE
                     GELALAEAVIYLATAPKSNATYVAFGKAMADAREYGSLPVPLHIRNAPTKLMKDLGYG
                     DGYQYDHDLPDRFSGQQCLPDELAGREYYEPTEQGYERTVAERMRWWAELREKLKRDK
                     GGE"
     gene            complement(6194..7249)
                     /locus_tag="P5179_07750"
     CDS             complement(6194..7249)
                     /locus_tag="P5179_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95148.1"
                     /translation="MIRVGMIGCGSMAASHATTFRPLDGRMRFTAFADIELERAEAQA
                     KHAPGAIAVTDYHDLLDAVDAVVIALPHDLHYPVGAEALRAGKHVLMEKPLALNREEC
                     RALVEMDRSPDPVLMVGYVMRHEPLWTELGEAIRTKRFGEVFHVSIWTEQYTDDSRGK
                     WLGQAARLGGGQLFSHGCHYIDLLLHWLGEPVSGTHVGTNLCTPWMEREGTSNVSLKF
                     ASGATAYHFGTWGARGSRLRYSVHAHTDQGMLELDFADGTLTLFRDRSGSDLPTLQAL
                     AAPGETMESPNRVVIARRSGAAAKPVVAQMAAFLDCIEQRKKPEISARQSVRSLEVIW
                     RLYEAEETGVVADLRGV"
     gene            complement(7434..>7871)
                     /locus_tag="P5179_07755"
     CDS             complement(7434..>7871)
                     /locus_tag="P5179_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="can hydrolyze p-nitrophenyl sulfate; contains a
                     specific cysteine that is converted into
                     C{alpha]-formylglycine upon activation with the anaerobic
                     sulfatase-maturase; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRS95149.1"
                     /translation="APERFGVQVGAVVDRPVGLEDVMPTCLDFAGVPVPGGVDGRSLL
                     PLLRGEEAPWREYIHIEHAPLHHTLTDGNEKYVWFVADGREQFFHLPENPDERFDVAK
                     HPRDAARVELWRTRLVVELKDRPEGFSDGERLIPGRPYPAVVG"
BASE COUNT         1474 a         2499 c         2451 g         1447 t
ORIGIN      
        1 gggagacgcg cttcatcagg cgcgggagag atactggcgt gttctcgtgt cgattctgat
       61 cttttcgccg acgccgagga acaacggcac ctgcaccact gccccggttt ccagcttcgc
      121 cggtttggtg ccgccggttg cggtatcgcc gcgcacgccc gggtcggtat ccgcgatggt
      181 aagcgtcaca tggttcggca gctccaccag aagcggcatg tcgtctccga tgatcaccgt
      241 gacgacttcg ttctccttca ggaggtccgc gatgtcatcc acgatctcgt gcggaaggtg
      301 gatctgctca tacgtctccg tatccatgaa gacgtaatga tcgccctcct tgtagatgta
      361 ctgcatgtcg cggttttcca cgcggatctc ttccaccgcc tccccggact tgaaggtacg
      421 gtcgatcacc gcgcctgtct ggacgttttt caggcgcgtc cgcacgaatg cgccaccctt
      481 gccgggtttg acgtgctgaa attcgacaat gaagtagatg ttgccgtcca tgcggaacgc
      541 gaacccgttc cgaaaatcct gcgtagttgc caacgagtgt tcctcctgat gacgctgcgc
      601 cttttcgcgg gcgcggttgt atgtgaatca aacgatacgc aattccttgg gcgtcgagca
      661 gagcacgcgg catcccttgc cggtgactac tgcgagatcc tcgatgcgaa cgccgcccca
      721 gccggtcagg tagatgcccg gctcaatcgt gattaccatt ccctcggcca gtgtctcctg
      781 gctgcgccac gaaatggacg gcccatcgtg cacccgcagg cccacaccgt ggccgaggga
      841 atgcccgaag gcttcgccga acccctgctc ctgaatatag gaccgggcca cgctgtccag
      901 ctccacgcac gaaagcccgg gcctgacggc cgcaactgcc cgcatctgag cttccaggac
      961 gatgccgtat accttctgca gtttctttga ggccttcccc acggcaaccg tccgggtcat
     1021 gtcgctcgcg tacccatccc gctgcgcacc gatatcaaac gtaaccatgt ccccgttccg
     1081 gattcgccgg tcggaaggct gcgcgtgggg aagcgcggcg tttggcccgc ttgcgacaat
     1141 tggcgggaac gccatccggt cggcgcccag ttttctcacg cgatattcca gctccgtggc
     1201 gaggtcgcgc tccgtcacgc cgggcttcac cagcggcagc acctccgcga atgcggcgtc
     1261 tgcaatggcg gcggagcggg caatggcggc gatttcggcc tcgtctttga cgaaaagctc
     1321 tcgctcgata atctgctctg ttgcgaccca ctgcacgctg agcggcgtgt cgccatcctt
     1381 gaacgggctt gtgagcgcgc ggtggtcttt caccgtcagg tgcgcgcttt caaagcccac
     1441 acgggagtcc ccccaccccg ccgcaagctc acgcacgcag gcaaccagac cgttctgcgt
     1501 gataacggcg cgccagtccg gcgcctgtac cgccgcctga tcgcggtaac ggaagtctgt
     1561 caggagcact ttttcgcgtg cacccaccac gagccacgca ctcgacccgg agaaaccgga
     1621 cagccagcgg acgtgctccg ggcacgttac gaggaatctg tccacgcgtt ccgcgcgcat
     1681 cgcgcgacga agggccacaa ttcggggttg aggcaacgtt acctcgccag agggaaagtt
     1741 acggcacgca cccgggaacc tgaaccggcc ggcggatggc aatgaataga ccggaactgc
     1801 cacaggaatg gcagtcaaaa agaataccat ttccagcggt ttgggtcaat tctcaggctt
     1861 gttgtcctgc gtcacgttga ccgtttggaa caccgcgggc tatattccaa gttccagata
     1921 cagtcgcgaa aagagaggga cgaatgggta tccgcacggc cattcaaggg ctgcgttccg
     1981 caatgcgggt tattgcgcac gcgggtcggg cggtctggct gtttttttcc gaacggagac
     2041 ccgctccgaa acccgaccac ccccttgtgg tgaaggtggt gtaccccggt ggcgccgcca
     2101 cgagggattt ctcgtttccc ctgcggacgg tctggaaggc gaaaatcgcc gtagcggttg
     2161 tgtttgtgct gtttttcgtc ggtctcgcgg caattggtga cgcggcgtac tcgcggtgga
     2221 ggctatacca cctcaacgcg caactgcgag aggcacagac aaacgagcaa ctgacactgg
     2281 aaatgcggcg gcaactcgcg gagatttggc tgattaacga gcgtctgcag cgcatgctcg
     2341 ggcgccacga gagctatcgc acgcgtcaga cggtggtgta ccgcctgccc cagggcctcc
     2401 ctgtctcgcg gtgggtgggt gttgcgttcg gtgtgccaaa agcgctgccg gaggatatgc
     2461 gcgcctgtta cctgtgcgcg cccggctcac tcgtgctggc gaccgccgat gggaaggtga
     2521 ccgatctctc gtgggacccg agaaacgggg acgtcgtcac catcgactac ggcgagggcg
     2581 ttactgcgcg ctacggcagg gacgctctgt tcttcgtgga aaaggacgag cttgtgcgga
     2641 aagcgcagcc cataggcgta atccacgccc ccgactccct gagcactccc ttgctgttct
     2701 atcaggtaac tgctgaaggt gaagaggtag aaaccgtgcc ttacatggtg gtgggaggca
     2761 caaccggtat ctacacggaa tagccggggc ggtcacgatt tcggaaaggc acatcagatg
     2821 gcaaagtttc aggctgtgac gagcgcgacg atcaacacga ttatcgggga aaactcccgc
     2881 gttgtgggtg attttgacat tgccggcagc atcaaggttg agggatttct gaaaggaaaa
     2941 ctcaccacgt cggaacggct ggtaatcggc ctgggcggcg ttgtgctggc cgatgcaaat
     3001 gtgcgcgacg ccatcatcgg cggcactttc accggcacgc tgtacgcgga aacccgcgtg
     3061 gagatggaga gcactgccaa ggtgcggggg gatgtcagaa ccaaacaact ggtcatccac
     3121 gaaggggcgg aatacgaggg gaacatctcg cgcggggagg aaccatccgc ggaggccgaa
     3181 ggggatacgg ggtaggtggt cgccagcacc cgtggccgcg atgggggcag gaagttctgg
     3241 aatgccaaca aggcgccatc ctacgtacaa ccgtgcaaaa cgtgtatctt ttcccgacag
     3301 acactgttgc cgcccggata cgccggcatc cgggaacctt ctgctgcgtc cggccgttgt
     3361 cgaggatacg cacacacagg gaaacctgat tgaaagaagg agaccagaat gaactatcag
     3421 cgtcatgcat ggggctgggc gcttgccgcc gcgtgcgcgg tgggcctctt gaacgcgccc
     3481 gcacacgcaa tcgccggttt cggcattcat gccggcaaag acttcgtgtc catttcggaa
     3541 gggagcttcg atcgggcaaa gtttgaggct gcgggaacac gcctcggcct gaccggggtg
     3601 aactgggcca actggaactc cgtcaccctc acccgcaagg agatctccag cccgtggttg
     3661 attggcgtgc acgcgtacat cgatgctatc ccctacatcg atcttgaagt atccgttgac
     3721 ggtgccattt cgcagtaccg cgtggattac cgctccacgt tgaacccggc ggtgaatgaa
     3781 agcaaggacg tgtattttgg ccgtgtcggt gcgtacggca cggtccggcg tgatatcgtc
     3841 aagtttccgc cggtggtccc cattgccgcg ctttacttcg gtggcggact gtcgtacaac
     3901 ttcatcgctc cggtggccgg accggacctg atcgtgaacg cgtacggcac cgacaacccc
     3961 accacacggc aacccgacat cgcaaagctg gtcgagcgcg agggaacctt cggctggcac
     4021 gggcttgtcg ggttccgcat caagccgccg gtggttccca tcgcgttgcg gctggaaggg
     4081 aaatacacat ccaccggaat cagcacgtat gagcggccca aaagcatatt cagcgcgtat
     4141 ctcggcacgt cgttcgcctt ctagcgtttg ctgagattcc tctggacggg cggtatcgcg
     4201 atcggtaccg cccgtttttg ttacaggcgt gcgggagggc cgttgatcgt gagcgaaggc
     4261 ttgaccaggg agtccgagat tacgtgtccg cagtgtggtt tcaacaaggt ggaaacaatg
     4321 cagatcgatg cctgccagta tttctacaaa tgcgagggct gcggtgtggt gcttcgcccg
     4381 aaatccgggg attgctgcgt cttctgttcg tacggctcac acccgtgtcc acctgtgtag
     4441 cagcaaaggg cgagcaacgc atgaccctgt gaaccgtgcc gacaggcccg attgccttcc
     4501 gagtggaagg gaagtatact tccagcggaa tccgcgcctg cgaacgcccg aagggcgcat
     4561 tcgacggctg cttgaggggg tagttcgccc cttggcgagc gttgaggttc ctgcggacgg
     4621 gcggtatcgc ggtcggtgcc gtccgttttc ccttgcggtc aggagtcggc catgagtgag
     4681 gacgaggaac tcatagaaac ggatctgttc gacggagtgg agaagcccga cacgaccgcg
     4741 ccgctcgccg acagaatccg cccgcgcacg ctggcagagt tcgccggcca gcggcatatc
     4801 atcggggagg gcgcgcttct gcgccgcatg gtggagcagg accgcctcgt atctctcctc
     4861 ttctggggcc cgcccggcac cggcaagacc actctcgcgt tcgtgctggc gcgcatgctc
     4921 aacgcccggt tcgttacgat gagcgccgta acgagcggag tgaaagatgt gaaagccgtc
     4981 atcgagcgcg ccgcgatgga aaaacggcga gggcggcgga cgatcctctt cattgacgaa
     5041 atccaccgct ttaacaaagc ccagcaggac gccctcctcc cgcatgtcga acgcggcacg
     5101 gtgatcttca tcggcgctac cacggaaaac ccgtcgttcg acgtgaactc ggctctcctc
     5161 tcccgctgcc gggtgtttgt tctgaagcag cttctgccgg aagacatcga acagatcgtt
     5221 cgtcgcgcgc tcgctgatcg agaccggggg ctgggcggcc ttgagctcgt gatggacgac
     5281 gacgcggtgg atgcgctggc aaaactggcc gacggcgacg cccggattgc tctcaacgcc
     5341 ctcgaagcgg cggcgaacgt ggtttctgcg ggggaccggc acatcacgaa gcaggtcatc
     5401 atcgaagcgc tccagcagcg tacatacgac tatgaccggg cgggagaagg gcactacaac
     5461 ctcatcagcg ccctgcacaa aagcgtgcgc agttccgacg tcaacgccgc gctgtactgg
     5521 ctcgcccgca tgcttgacgc aggagaggac ccgcgctacc tcgcccggcg gatggtccgc
     5581 atggcggtgg aggacatcgg cctcacggac ccgcgcgccc tgagcgtcgc cctcgccgcg
     5641 cacgagacct accacatgct cggttcaccg gaaggggaac tcgccctcgc ggaggcggtg
     5701 atctacctcg cgaccgcgcc caaatccaac gccacctacg tcgcgttcgg caaggccatg
     5761 gccgatgccc gcgagtacgg cagtctgccg gtaccgctcc acatccggaa cgccccgaca
     5821 aaactcatga aggacctcgg ctacggcgac ggctaccagt acgaccacga cctgccggac
     5881 cggttcagcg gccagcagtg cctgccggac gaactggcgg ggcgggaata ctacgaaccg
     5941 acggagcagg gctacgagag aactgttgcc gaacgcatgc gctggtgggc ggaactgcgt
     6001 gagaagctga agagggacaa aggcggggag tgaaacgggt tctcaccacg aacgacacga
     6061 aacacaccaa aggccgatag acggctcagg cgagcgcgca tcgcgagtgt atctgtcgta
     6121 taggtccctt tggacgaata ccggcggcag cagggtttga tggtccgcgt gagtttggag
     6181 ggacgcgcct ttctcacacc ccccgcaaat ccgccactac gccggtctcc tccgcctcgt
     6241 agagacgcca gatcacctca aggctgcgca ccgactgccg ggcgctgatc tcaggcttct
     6301 tccgctgttc gatgcaatcg agaaacgccg ccatctgagc caccaccggt ttcgccgccg
     6361 cacccgaccg ccgcgcgatc accacccggt tcgggctctc catggtctcg ccaggtgcag
     6421 ccagcgcctg cagggtgggc agatcggagc ccgagcggtc gcggaacagc gtcagagtcc
     6481 cgtcggcgaa gtcgagttcc agcatgccct gatccgtgtg cgcatggacg gaataccgca
     6541 gccgcgaccc ccgcgcgccc cacgtgccga aatgatacgc cgtcgccccc gaggcgaact
     6601 tcagcgacac gttgcttgtg ccttcgcgct ccatccaggg cgtgcagagg ttcgtgccca
     6661 cgtgcgtccc gctcaccggt tcgcccagcc agtggaggag caggtctatg tagtggcacc
     6721 cgtggctgaa taactgcccg ccgccgagcc gggcagcctg tccaagccat ttcccgcggg
     6781 agtcatcggt gtactgctcc gtccagatag acacgtggaa cacctcgccg aaccgcttcg
     6841 tgcggatggc ttcccccagc tccgtccaga gcggttcgtg gcgcatgaca tagcccacca
     6901 tcagcaccgg gtcgggcgag cggtccattt ccaccagcgc gcggcactcc tcccggttca
     6961 gcgcgagggg cttctccatc agcacgtgct tgcccgcgcg gagtgcctcc gcgccgacgg
     7021 ggtagtgcag gtcgtggggc agcgcgatca cgaccgcgtc caccgcgtcg agcaggtcgt
     7081 ggtaatccgt caccgcgata gcgcccggcg cgtgcttcgc ctgcgcctct gcgcgctcaa
     7141 gctcgatatc agcaaacgcg gtgaagcgca ttcgcccgtc cagcggccgg aacgtggttg
     7201 cgtggctggc tgccatactg ccgcagccga tcattccaac gcggatcatt ctgtgcgcct
     7261 ccgtcgactt tcggtgtacc cggcccagcg gctccattat gggtcctatg ggtcctatga
     7321 gtcggatggt aagctgtctt tggagtgcgg cggcgtgccg ccgctttcca agcgcaggca
     7381 agcctgccgg cacttcgaca ggctcagtga ccggcacact acgtgagcgt cggtcagccc
     7441 accactgccg ggtagggtct tccgggaatc agccgttcgc cgtcgctgaa gccttccggc
     7501 cgatctttca gctccaccac cagccgtgtg cgccagagct ccacgcgggc ggcatcccgc
     7561 gggtgtttcg ccacatcgaa gcgctcgtca gggttttccg gcagatggaa gaactgctcc
     7621 cggccatccg caacgaacca gacgtatttc tcgttgccgt cggtcagcgt gtgatgcagc
     7681 ggcgcatgct caatgtggat gtactcccgc cacggcgctt cctcgccgcg cagaaggggc
     7741 agcagactgc ggccatccac gccgcccgga accggcacac cggcgaaatc gaggcaggtc
     7801 ggcatcacgt cttccagccc caccggacga tctaccaccg ccccgacctg caccccgaat
     7861 cgctccggcg c
//
LOCUS       DAOTNG010000135         7871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8178_length_7871_cov_5.423490, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000135 DAOTNG010000000
VERSION     DAOTNG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8178_length_7871_cov_5.423490"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="P5179_07760"
     CDS             <1..1494
                     /locus_tag="P5179_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRS95150.1"
                     /translation="EPGDWYHGYPEYHVERDDDKCIRCLVCVRQCPYDCTFYDPHRHG
                     VYNVHENCVNCRRCEFMCPTSAIRITKWGAGFRDNDNWKQKTIRTVYKQADSGAVSLT
                     GLGNDREYVSYFDRMVIDASQVTNPSIDPLREPMELRAYIGKKPERHTNPPEVYPNLK
                     AELPVLFAPMSFGAIAFNFQRGLAMAAKEVGTYFNTGEGGLHPDLRQYGDHAIVQIAS
                     GRFGVSLDYLNNAAACEIKIGQGAKPGIGGHLPGEKVTWDVSRTRMIPEGSDALSPAP
                     QHDIYSIEDLRLLIYGLKEATEYTRPVSVKIAAVHNVAAIASGIVRAGADIVVVDGFR
                     GGTGAAPTVIRDHVGIPVEVALAQVDQRLREEGIRHQASLIAAGGIRSSADVIKGLAL
                     GADAFYIGTAAMVAVGCTACQQCHRGRCSWGITTNRPDLAPRLQPEVAAERLTNLLHG
                     WAHEIKEMLGMMGLNSIESLRGNRLKLRGVGMTQVELDTLGIQQAGL"
     gene            1497..2033
                     /locus_tag="P5179_07765"
     CDS             1497..2033
                     /locus_tag="P5179_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS95151.1"
                     /translation="MKRVYPREERCINCHLCEVACIVEHSETKLPAAAYHVEGLRFNR
                     EDSRTICDPAEAGTAGVPKPLNRTLVQTAGYLSLSTNCRHCEEPDCVLACKNGSLYVA
                     EDGRVLVQEDKCVGCWMCVMACRYGAISRNPTRSNVPAVAFNGINHHCDLCPEREVPA
                     CVWVCPTNALVFEDRDDQ"
     gene            2030..3286
                     /locus_tag="P5179_07770"
     CDS             2030..3286
                     /locus_tag="P5179_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS95152.1"
                     /translation="MNHVIIGNSFAALFAIEAIRRTDQDAEITVISREPQHVYSRAMI
                     HEMLAGMVREPMVYLRDWDFYKRMRVRTMLGRTVTKINPGLQTVSTEEDETPYDRLLI
                     ATGGKPFVPPLNGLQDITYHSFITLEDAKKLKAACENRRNAVVLGAGLIGLQCAEALR
                     HMGLNVAVVEMADTVLPMALDTESSQTIAKELADEGIAVHTGDTIDLIISNDGHPTEC
                     KLRSGGTIPCDLLVIAVGVRPNIDLAKDAGITTDRGIVVNEHMETSAEHIYAAGDCAQ
                     GPEIVTGITMPIPVIPVASTQGMIAGYNMAGKRRRYGGALALNAMQFGGVQVISYGFV
                     KDERDGEVLKYIDPPRKIYRKVIIKNGRITGAIFVKDVDRAGIFRHLMEKRIDVTPFK
                     EHLLSPHFGVAHLPAEIRADMFTRPQ"
     gene            3324..4475
                     /locus_tag="P5179_07775"
     CDS             3324..4475
                     /locus_tag="P5179_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HRS95153.1"
                     /translation="MPSMRGNRHFVRRPHQEKDISACGIIGFINRDGTRVSGDAIIRS
                     IANMHDRGNGLGGGFAAYGIYPEHADAYALHIMYDDERAIADTEALIRNTLETEHAET
                     IPVHPNGTVTEYPEFRRYFCHPPDVDERDADEYMVRLVMRINAKIEGAYVVSSGRNMG
                     AFKGVGFPEDLAEFFCLQEYEAYLWTAHNRFPTNTPGWWGGAHPFTLLDWSIVHNGEI
                     SSYGINRRYLESFGYACTLRTDTEVVAYIFDLLVRRHGLSFEQAATVMAPPFWQDIDR
                     MPPDEAAEATALRQTYASAALNGPFAFLFGYNGGLIGLNDRVKLRPLVVAERGDTVYM
                     ASEESAIRVVSPDLDTLFYPVAGQPVIARLYSETAENTSVAHGGTVHGD"
     gene            4465..5244
                     /locus_tag="P5179_07780"
     CDS             4465..5244
                     /locus_tag="P5179_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95154.1"
                     /translation="MATEPTLAPSVTEPAVISAAGLSYRHLNEQVRSAIRSGATEITL
                     NDVMGHRYIGTGIHAPGVTIHVNGVPGEDLGAFLDGPHIIVHNNAQDGVGNTMNEGRI
                     EIHGQAGDVVGYGMRGGRILIRGDVGYRVGIHMKGYRDKQPLIVTGGCAGNFYGEYMA
                     GGCQVLLGIGQSEKKPVVGQYCASGMHGGVMYIRGELDDWKLGKEVQQIPLDRHDREF
                     LKPVLEDFCETFAVKEKLLEFDRFVRIAPVSARPYGRLYVY"
     gene            5254..5937
                     /locus_tag="P5179_07785"
     CDS             5254..5937
                     /locus_tag="P5179_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRS95155.1"
                     /translation="MNFTNSLKALDKLIGSTLRALRESQKLYDAEGIQNEELEKTLKK
                     TGAELRELRKSFSAILAAHAGTFEMVRYLNEGLRMEYQTILDYERYVNVVEDATLATR
                     LRDFGAEERRHAHALSLKITELGGEPKFTVAHERRPDLTAFELLQQHLATEREAVKYY
                     DMGLEKFDDPGFRWLIGKVKVDEEEHLKRLEELIEQYRDTAVLVQESKNFKWIDPYMG
                     KPGDRAWIE"
     gene            6150..6638
                     /locus_tag="P5179_07790"
     CDS             6150..6638
                     /locus_tag="P5179_07790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS95156.1"
                     /translation="MPKSYSAPPSMSIDPAVCYHAKIRTSRGDIRIELFAKDAPVTVN
                     NFVFLARDGFYNGLTFHRVITGFVIQGGCPNGDGRGGPGYRFADEVDKNPHRHTAGAL
                     SMANAGPGTNGSQFFITHTPQPHLDGKHTVFGRVIEGMDTVLSVRQGDEIQRVEIVEQ
                     PA"
     gene            6730..>7871
                     /locus_tag="P5179_07795"
     CDS             6730..>7871
                     /locus_tag="P5179_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS95157.1"
                     /translation="MSAVVSLPGRKTSAISHAAALLLCALAGVPCGCSSNPASPQNKR
                     VRVPTALFHADTSLSVEDAPWSLAIADFSGDSVPDVAVACLGGKSVVMYTNGEGGAIF
                     HRQKKAPADTTFRFSGGFRPRSVATADVNADGLPDLVVALAQDSVVSGLDRIVVLLNQ
                     RVSTGSSAYSVGADYVAGSGVRDLFVGDLDPGDGGVEIVGASSVPGVAPVVLARAARV
                     ARAGEAIDSTINLLGFSPADGASNLGLRTPVSFWFDTEVKNMSALANLDSQWVQITGT
                     IDNSGQTRRIPVSVAKQTRRVNYASYTEYVLTLLTPYRPHETVTVSLTGRILSLQSND
                     RRTIALTNPRSWSFSTEGTRIVSSIPANGATHVPTTSPVSLRFNFWL"
BASE COUNT         1620 a         2314 c         2474 g         1463 t
ORIGIN      
        1 gaaccgggcg actggtacca cgggtacccc gaataccacg ttgaacgcga cgacgacaag
       61 tgcatccgtt gccttgtctg cgtgcgtcag tgtccatacg actgcacgtt ctacgatccg
      121 catcgccacg gggtgtacaa cgttcatgag aactgcgtaa actgccgccg gtgcgagttc
      181 atgtgcccga caagcgccat ccggataacc aaatggggcg ccggttttcg tgataacgac
      241 aactggaagc agaagaccat ccgcacggtg tacaaacaag cggattccgg cgcagtaagc
      301 ctgactgggc tcggaaacga ccgcgagtac gtctcctatt tcgaccgcat ggtgattgat
      361 gcgtcgcagg ttacaaaccc cagcattgat ccacttcgcg aaccgatgga gttgcgcgcg
      421 tacatcggaa agaagccgga gcgacacacc aatccgccgg aagtgtaccc gaacctgaag
      481 gcggaattgc cggttctttt cgccccgatg tccttcggag ccattgcgtt caacttccag
      541 cgtggccttg ccatggcggc gaaggaagta ggcacgtatt tcaacaccgg agagggcggc
      601 ctgcaccccg acctccgcca gtacggtgac catgcgatcg tgcagatcgc ctcgggccgg
      661 ttcggcgtaa gcctcgatta tctcaacaac gccgcggcct gcgagatcaa gatcgggcag
      721 ggggccaaac cgggcatcgg tgggcatctg ccgggagaaa aggtgacgtg ggatgtctcg
      781 cgcacgcgca tgatcccgga aggcagcgat gctctcagcc ccgcgccgca gcacgacatc
      841 tactcgattg aagatctgcg ccttctcatt tacgggctca aagaggccac ggaatacacc
      901 cggcccgtga gtgtcaagat cgcggccgtt cacaacgtcg ccgccatcgc gagcggcatc
      961 gtccgcgccg gtgctgacat agtggtggtt gacggttttc ggggcggaac cggagcagct
     1021 cctaccgtga tccgggacca cgtcggcatc ccggttgagg tggcgctggc gcaggttgac
     1081 cagcggcttc gtgaggaagg tattcgccac caggcttcgc tgatcgcggc gggaggaatt
     1141 cggagctccg cggatgtgat caaaggcctc gcgctgggtg cggatgcgtt ctacatcggc
     1201 accgcggcga tggtggcggt ggggtgcacc gcgtgccagc aatgccaccg cgggcgctgc
     1261 tcgtggggca ttaccaccaa ccgtccggac ctggctcccc gactgcagcc ggaggtagcg
     1321 gcggagcgat tgacgaacct tctccacggc tgggcgcacg agatcaagga gatgctcggg
     1381 atgatggggt tgaactccat tgagagcctc cgcggaaatc gcctcaaact ccggggcgtc
     1441 ggcatgactc aggtcgaact ggacactctt ggcatccaac aggcagggct ctgacgatga
     1501 agcgcgtgta ccccagagaa gaacggtgca tcaactgcca tctctgcgaa gttgcgtgca
     1561 tcgtagagca ttccgagacg aagctccctg ccgcggcgta ccacgtggag ggactccgct
     1621 tcaatcgcga ggacagccgg acgatctgcg atccggcgga agcagggact gcgggtgttc
     1681 ccaagcctct caaccggact ctggtgcaga cagccgggta cctctcgctc agcaccaatt
     1741 gccggcactg cgaggagccc gattgcgtgc tcgcatgcaa gaacggctcc ctgtacgtgg
     1801 ccgaggatgg gcgcgtgctg gtacaggagg acaagtgcgt gggctgctgg atgtgcgtga
     1861 tggcgtgccg gtacggcgca atctcacgca atcccacccg ctcgaatgtg cccgccgttg
     1921 cgttcaacgg catcaaccat cactgcgacc tttgccctga acgggaagtc cccgcctgtg
     1981 tctgggtgtg cccgacaaat gccctcgttt ttgaagacag agacgaccaa tgaaccacgt
     2041 aatcatcggg aattcgtttg cagctctgtt cgctatcgag gcgatcaggc gaactgacca
     2101 ggacgcggag atcaccgtca tcagccggga accgcagcac gtgtacagcc gggcgatgat
     2161 ccacgaaatg ctggccggga tggtgcggga accgatggtg tacctgcgcg actgggattt
     2221 ttacaagcgg atgcgcgtaa ggacgatgct cgggcggacc gtaaccaaaa tcaatccggg
     2281 gttgcagacg gtatctaccg aggaagacga gacgccatac gaccggctgc tcattgccac
     2341 aggtgggaaa ccgtttgtgc cgcccctgaa tggcctgcag gacattacgt atcactcgtt
     2401 catcactctc gaggacgcca aaaagctgaa agccgcgtgt gagaaccggc gcaacgcggt
     2461 cgttctgggt gccgggttga tcggtctcca gtgcgcggag gcgttacggc acatgggact
     2521 taacgtggct gtggtcgaaa tggcagatac cgtgctgccg atggcgctgg acacggaatc
     2581 cagccagacc atcgcgaaag agctcgccga cgagggtatc gccgtccata cgggtgacac
     2641 gatagacctc attatcagca acgacggcca ccctacggag tgcaaactgc gcagcggggg
     2701 aacgattccc tgcgatctgc tcgtaatcgc ggtcggcgtg cgccccaata tcgatcttgc
     2761 aaaggacgcc ggaatcacaa ccgaccgcgg gatcgtggtc aacgagcaca tggaaacgag
     2821 cgcggagcac atctacgccg cgggggattg cgctcaggga ccggagatcg ttaccggcat
     2881 aacgatgccg attcccgtca ttccggtcgc ttccacgcag gggatgattg cggggtacaa
     2941 catggcgggg aagcgccggc ggtacggcgg cgcgttggcc ctgaatgcca tgcagttcgg
     3001 cggcgttcag gtgatcagct acgggttcgt gaaagatgaa cgcgacgggg aagtgctcaa
     3061 atacatcgac ccaccccgga agatctaccg aaaggtgatc atcaaaaacg ggcgcattac
     3121 cggagcaatt ttcgtcaagg acgtggatcg ggccggcatt tttcgccacc tgatggaaaa
     3181 acgcattgac gtaacaccgt tcaaggagca tttgttgtcg ccacatttcg gcgtagcgca
     3241 cctgcccgcc gaaatccgtg cggacatgtt cacccgcccg caataagaac cagttgcctg
     3301 cggatcgaag gagaacacgg ggcatgccat caatgagagg aaaccgccac ttcgtccggc
     3361 ggccgcatca ggagaaggat atctccgcat gcggcatcat cggcttcatc aatcgcgacg
     3421 gaacgagagt gtccggtgac gcgataatcc gctccatcgc caacatgcac gatcgtggca
     3481 acggtttggg cggcgggttt gcggcgtacg ggatctatcc cgaacacgcc gacgcatatg
     3541 cgttgcacat catgtacgac gacgaacgcg cgattgcgga cacggaagcg ttgatccgga
     3601 ataccctgga aaccgagcat gcggaaacga taccggtcca ccccaacggc accgtgacgg
     3661 agtaccctga gttccgccga tatttctgcc accctccgga cgtggacgaa cgtgatgcgg
     3721 atgagtacat ggtgcgcctc gtcatgcgga tcaacgcgaa aatcgagggc gcgtatgtcg
     3781 tttccagcgg ccggaatatg ggggcattca agggggtggg gttccccgag gacctggcgg
     3841 agttcttctg cctgcaggag tacgaagcct acctgtggac ggcacacaat cgttttccga
     3901 cgaacacccc gggttggtgg ggcggcgcgc acccgttcac tctgctcgac tggtccatcg
     3961 tacacaacgg cgagatatcc agctacggta tcaaccggcg ctatcttgaa agcttcggtt
     4021 atgcctgcac cctgaggacg gataccgaag tggtggcgta catattcgat ctccttgtcc
     4081 gccggcacgg actttcgttc gaacaggcgg ccacggtgat ggcaccaccg ttctggcagg
     4141 acatcgaccg gatgccgccg gacgaagccg cggaggcgac ggcgctccgc cagacgtacg
     4201 cctcggcagc gttgaacggc ccgttcgcgt ttttgttcgg gtacaacggc ggcctcatcg
     4261 gcctgaacga ccgggtcaaa ctgcggccgc tggtggtggc cgaacgggga gataccgtct
     4321 acatggcgag tgaggagtcg gcgattcgcg tggtgagtcc ggatctcgac accctctttt
     4381 accccgtcgc cggccagcct gtcatcgcgc ggctctattc ggagacggca gaaaatacat
     4441 ctgttgcgca cggaggcacc gttcatggcg actgaaccca cgttggcacc gtccgtaacc
     4501 gaaccggcgg tgatctccgc agcaggtctt tcctaccggc accttaatga acaggtgcgg
     4561 tcggcgatcc gttcgggcgc gacagaaatc actctcaatg acgtgatggg tcaccggtac
     4621 atcggcaccg gcatccatgc cccgggggtt actatccacg tgaacggcgt tccgggcgaa
     4681 gaccttggcg cgtttctcga cggcccccac atcatcgtgc acaacaacgc acaggatggc
     4741 gtcggcaata cgatgaacga aggccgtatt gagatacacg gtcaggccgg cgacgtcgtg
     4801 gggtatggca tgcggggcgg gcgcattctc atccggggcg atgtcggcta tcgcgtcgga
     4861 attcacatga agggttaccg cgataaacag ccgctcattg taacgggtgg gtgcgcaggg
     4921 aacttctacg gcgagtacat ggcgggcggt tgccaggtgc ttctcggcat cgggcagtcg
     4981 gaaaaaaagc cggttgtcgg gcagtactgc gcttcgggta tgcacggcgg ggtgatgtat
     5041 attagagggg agctcgacga ttggaagctg gggaaagaag tgcagcagat tccgctggac
     5101 agacacgatc gcgagtttct gaagcccgtt cttgaggatt tctgcgaaac attcgcagtc
     5161 aaggaaaaac tgctggaatt cgaccggttc gtcagaatcg caccggtgtc ggcacgtccg
     5221 tacggacgcc tgtacgtcta ttgaggagcc accatgaact tcaccaactc cctgaaagcg
     5281 ctggacaaat tgattggttc gacccttcga gcgctcagag agagccagaa actctacgac
     5341 gcagagggca tccagaacga agaactggag aaaaccctca agaaaaccgg cgccgagttg
     5401 cgggagctgc gaaagtcatt ttccgcaatt ctggcagccc acgccggaac gtttgagatg
     5461 gttcgttacc tgaacgaagg tcttcggatg gagtaccaga ccattctgga ttacgagcgg
     5521 tacgtgaacg tggtggaaga tgccacgctg gcaacccgtc tcagagattt cggtgctgag
     5581 gaacgccgcc acgcccacgc gctttccctg aaaatcaccg agctgggcgg cgaaccgaag
     5641 tttaccgtcg cccatgagcg acgcccagat ctgacggcat ttgaactctt gcaacaacac
     5701 cttgccaccg aacgggaggc cgtgaagtac tacgacatgg ggctggagaa attcgacgac
     5761 cccggattcc gctggctcat cggcaaagtg aaggttgatg aggaggagca cctgaagagg
     5821 ctggaggagt tgatagaaca ataccgcgat acggcggtac tcgtgcagga atcgaagaac
     5881 ttcaagtgga ttgaccccta catgggcaaa ccgggcgacc gcgcgtggat tgagtgacca
     5941 cgggcagggg ggctcgcccg gtctgttgtc cgcattcctc tttgtgccgc cgcgggcgtg
     6001 gagatctcgc cttcggggcg cccatccgca ccaccgccgc gcggtgcctg tcttcccacc
     6061 cgcgtcactc ggttggtatc tccccacgcg cagccgtatt tttccgaaag accctctcac
     6121 cagtttgtca tcccctcgaa ggagcaatga tgcccaagag ctactccgct ccgccgtcaa
     6181 tgagtatcga tccggcggtg tgctaccacg caaaaatccg cacgtcgcgc ggcgacatcc
     6241 gcatcgagct gttcgccaaa gacgcgccgg tcacggtcaa taatttcgtg tttcttgcca
     6301 gggacgggtt ctacaacggt ctgacgtttc accgtgtgat cacgggcttt gtcattcagg
     6361 gaggatgccc caacggcgat ggccgcgggg gcccggggta tcgcttcgcc gacgaggtgg
     6421 acaaaaaccc ccaccgacac accgccggag ccctttccat ggcaaatgcg ggaccgggca
     6481 ccaatggaag ccagttcttc atcacccata ccccgcagcc gcatctggac ggaaagcaca
     6541 cggtgttcgg gcgggtgatt gaaggcatgg acacagtgct ttcggtccgc cagggcgatg
     6601 aaatccagcg tgtggagatt gtcgaacagc ctgcgtgacc aggctgcctg tcttgccagg
     6661 ctgcctgtct tgccaggcgg cctgcgtaac caggccgccc gaatcacggg gatacgcgat
     6721 gggaagagga tgagcgcagt ggtatctctg ccgggcagga agaccagcgc gatcagccat
     6781 gcagcggcgt tgttgttgtg cgcgctggcc ggggtgcctt gtggttgctc gtcgaatccc
     6841 gcgtcgccgc agaacaaacg ggtgcgcgtt ccaactgcgc tgtttcacgc cgataccagt
     6901 ctctcggtgg aggacgcgcc gtggtccctt gcgatagcag atttcagcgg ggacagcgtc
     6961 ccggacgtcg ccgtcgcctg cctcggcggc aagtcggtgg tcatgtacac caacggcgaa
     7021 ggcggggcga ttttccaccg gcagaagaaa gcacccgccg atacgacttt ccgcttttcc
     7081 ggcgggtttc ggccgcgttc ggttgctact gctgacgtga atgccgacgg cctgcccgac
     7141 ctcgtggtgg cgctcgcgca ggattccgtt gtgtcgggtc tggatcggat cgtggttctg
     7201 ctcaaccagc gggtatccac gggtagttcc gcctattcgg tgggcgcgga ttacgttgcg
     7261 gggagcggcg tgcgcgatct tttcgtcggc gaccttgacc cgggcgacgg aggggtggag
     7321 atcgtcgggg cgtcgtccgt tccgggtgtt gcgccggtag tccttgcgcg ggcggcaaga
     7381 gttgcgcgcg ccggggaggc gattgacagc accatcaacc tgcttggttt ttctcccgcg
     7441 gatggtgcct ccaaccttgg tctccgcacc cccgtgagtt tctggttcga caccgaagtg
     7501 aagaacatga gcgcgctggc aaacctcgac agccagtggg tgcagataac aggcactatc
     7561 gacaacagcg ggcagacgag acgcattccg gtaagcgttg cgaaacagac gcggagggtg
     7621 aattacgcgt cctacacgga gtatgtcctg acgctgctca cgccgtaccg cccgcacgaa
     7681 acggtgacgg tttctctcac cggccgcatt ctgagtctcc agtcgaacga ccgccgcacg
     7741 atagccctca cgaatccccg ttcgtggtcg ttcagcaccg agggcacgcg tatcgtgagc
     7801 agcatccccg cgaacggggc aacccacgtg cctacaacgt cccccgtgtc gttgcgtttc
     7861 aacttctggc t
//
LOCUS       DAOTNG010000136         7864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8182_length_7864_cov_5.455372, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000136 DAOTNG010000000
VERSION     DAOTNG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8182_length_7864_cov_5.455372"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="P5179_07800"
     CDS             complement(<1..487)
                     /locus_tag="P5179_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95158.1"
                     /translation="MTKPLGAHMKGSRCRSGNTLTLVLFLAASWILPSTARAKSLGEI
                     VLSVPVDTVVEAGQQFLLPVRISGVTVLDSVTSFNFGVRVPLGIMLVDTAAVKGPALP
                     SGAGFSSAKRDSVIVVGGYWTLPLANNGTLFFLRCTPKANIPDNTSGEVRLERLSQNG
                     TL"
     gene            complement(488..694)
                     /locus_tag="P5179_07805"
     CDS             complement(488..694)
                     /locus_tag="P5179_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95159.1"
                     /translation="MRSVYWDLLHIVAFVLFLLFVAGVAANVAGYGPREWFLPLMIAA
                     AMSAAAVLATDQSRRWPWFRRGSQ"
     gene            complement(779..2146)
                     /locus_tag="P5179_07810"
     CDS             complement(779..2146)
                     /locus_tag="P5179_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRS95160.1"
                     /translation="MEQLPLVISPPLSSSHEFAAAAEVIRTLHAAGYDAYLIGGCVRD
                     MVLGLIPKDFDIVTSAKPEETQALFPRSLRLGAQFGVITVLANGLSFDVATFRREGNY
                     EDGRRPSWVEYADLEADVRRRDFTINGMAFDLSRSSVIDLCGGLADLREGIIRTIGDP
                     AERFGEDALRVIRAVRFAARFGFSLEETTRLALMSAAPSLGRIARERIWQELEFMMTE
                     EHREVAFRLLAELGLLPHVFGPDVVRSSETIVRTSARLEALASDASIVLVLACVLADG
                     VARAAPNESADRVVEPYDEEAASRAEAVLASLRAPRSVVDGVGALIARRMVWAAQNSL
                     REGRIAQLVRNDRTGALVSFWAADAASFGLADAVSRIRDIADRIIAEGKAHGGKSRMP
                     VNGTVLAEAGVPRDATMGELLAEAERLWLEGKLETEADVRRWLADAVRSADASPCPGN
                     SKPQA"
     gene            complement(2125..2610)
                     /locus_tag="P5179_07815"
     CDS             complement(2125..2610)
                     /locus_tag="P5179_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS95161.1"
                     /translation="MARHKTILVVDDNPSMRAILAIWLNRFVHLPFRLHVLTAPDGEE
                     AWRILGASRVDLLLANHHMPKTPGTQLIRRVKSNTRLRNVPCVLESIGELPDLAAEGL
                     SCGADFVISLLNNDKSDLESVLAAALGVSSQTETDPGRTPHDSVLTPQWWGTSWNSSP
                     L"
     gene            2792..4240
                     /locus_tag="P5179_07820"
     CDS             2792..4240
                     /locus_tag="P5179_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS95162.1"
                     /translation="MPTLHGRITDAANGELREAKVSVVDCEGGVRTPEGAVLKVGNGL
                     VSFYCGGEFSVPVKGGPCRIVVERGTEYEPLVKHLNVPWKGDLDVTLELRRWVDLPRM
                     GWYPGNTHIHYDQNERQPYERLRLEPHVNDFAVTVVSILQRWDLPYASNRFPLGAFTE
                     FSTAHHVVDVGEEVRHNFWEGGFGYGHLLVFRIHDQVEPLSRGMLVNDFNPDYPPLCL
                     ACDDAHRQGGIVVWCHNGLGMEAPVAAALGKLDALNLFDPYWMNPEYDLWYKMLNCGF
                     HLPASTGSDWFVCSNNRVYACTGTDFSYETWLAALKKGQTFITNGPALLLSVDGHGPG
                     AFLPCTAKRAIEVKAEWRSFHPIEVVEIVRDGFVIARKEFATGSREGSFVQTVLPETD
                     GWIAARCSGNARDSFDQAIFAHTSPVYVQTGALSPAREEAARFFADVIRGSVDWVNTK
                     GRFHNKQQRTTVLNLFREGAAVYEGMLRKAHT"
     gene            complement(4273..5226)
                     /locus_tag="P5179_07825"
     CDS             complement(4273..5226)
                     /locus_tag="P5179_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HRS95163.1"
                     /translation="MTASRLRLLTVMALLFCGCGGYQLESGLSALAPTRAVRLVFMRS
                     FGERGSSSGCFRQPQGIACASFGHWYVADTGNDRIQEFDGEGRYVREIGGFGWSDGQF
                     NRPAGIAAGRNLDIWVADTYNRRLVRLGQDLVWAGVINRDAEGGPLGYPNGIALNVDA
                     SIWCTDIDADRLIQLYQETPSELGARIQSTGILSDPAGVACSPDGNIVVADTGNDRIA
                     VFDRFGTLLRTWGEGILLRPRGVNVTPQGDILIADTGNNRLVFLNRMYHLTGEHGLRG
                     NEDGEFLEPCSVASDPAGNVVWVADTGNNRVQVFRVIRTTE"
     gene            complement(5223..6164)
                     /locus_tag="P5179_07830"
     CDS             complement(5223..6164)
                     /locus_tag="P5179_07830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS95164.1"
                     /translation="MAEITVLMPTYNRPEHLSAALETLAGQFEREVPFSVVVVDDGSV
                     PAVELDPGAFPFRLIFLRLEKNCGRSAARNAGLAVVDSPLVVFLDDDMRVLPGFIRAY
                     QEAIDPAGRAVGLGSVCFHPDIPRDALTLYLETRGIAKLKADEPIPFRYFLTYNSAVP
                     TFLLREAGGFSERVRAWGGEDIELAWRLQKHGARFVRVPQARAYHAHRRRLEDVLSLS
                     ESFARECMPVLFDEDPVFTTQLRADLLGPVRYAKRNRWKRSAIRFVTTPCVSRRVEAL
                     VKRHLGARWPMWVFDYLIASAWRRGLDQAHARGEERE"
     gene            complement(6157..7329)
                     /locus_tag="P5179_07835"
     CDS             complement(6157..7329)
                     /locus_tag="P5179_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS95165.1"
                     /translation="MNARNGGGIAVDMVVYNDLRHDARVFKEATSLIAAGYNVRVIGM
                     ETPSTPPLTGWEEVTVKRIPVGTSDSLRIRYACFWRRAFSALMERRARIIHSHDLDTL
                     FPAWLAASLTRAQLVHDAHELWVELPSLVGRPVVRAAWQGFAKTLIPRSDAVITVCEG
                     IADELRARYGVGAVVLRNLPKEIGPVEPAPIREMTGCPPSRVLVLYQGGFLPGLGIER
                     AIDTMTLLPDAHLVLIGAGPLRKDMEHRAAASPARDRISFLDAVPFKDLPPYTAACDI
                     GLFLGESAGMNLQLALPNKLFEYIAAGVPVVATGWPEIRRVVREYDVGVLVEPGCDLE
                     AVANAIRRAVSDRKRLSENCVKAARELTWERESDRLLEVYARLSARNSSPENRNDG"
     gene            complement(7326..>7864)
                     /locus_tag="P5179_07840"
     CDS             complement(7326..>7864)
                     /locus_tag="P5179_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95166.1"
                     /translation="REANLRTECLTGEHAHGALRETVAATFRRQGEAPPLPAQAWDDY
                     LALLSVCNNVATMGVFRGDAVLATIAIGFDPPTATGAGQSAYELIAGTTDEGIPVGAS
                     ALALCSAFEFASGKVGEFDFAGANMETIAHYKRGFGGTLTPYLAVEWSRSRRASFVAK
                     TAPAWKRKFRALLPKGSR"
BASE COUNT         1598 a         2475 c         2323 g         1468 t
ORIGIN      
        1 gcagagtgcc gttctgcgat aaccgttcca gtcgcacctc cccggaggtg ttgtccggaa
       61 tgttggcctt cggagtgcaa cgcagaaaga acaacgtgcc gttgtttgcc agcgggagtg
      121 tccagtagcc cccgacgaca atcacactgt cacgtttcgc ggagctaaac cccgctccag
      181 acgggagagc tggtcctttg acggctgcag tatctaccaa catgataccg agcgggacgc
      241 gaacgccgaa gttgaaggag gttacggagt ccagaacggt gacgccgctg atgcgtaccg
      301 gcaacaaaaa ctgctgtccc gcttccacga ccgtgtcgac cggcacgctc agaacgattt
      361 cgccaaggga tttcgcgcgt gcggtggacg gcagaatcca agaagcggcc aggaacagca
      421 ccagcgtcaa ggtgttaccg gaccgacatc gggacccctt catgtgcgcc cccaacggct
      481 ttgtcattca ctgtgaaccg cgccggaacc agggccagcg tctgctttga tccgttgcca
      541 gcacagccgc cgcggacatc gcagcggcaa tcattagagg cagaaaccat tcccgggggc
      601 cgtacccggc aacatttgct gcaacgcctg ccacgaagag gagaaacaac acgaacgcaa
      661 cgatatggag taaatcccag tagaccgatc gcatgccctt acgcttccca cgtgatcccc
      721 cccgagatcg agattgccca tctccaatat agcgcttcaa tcccagaaat gggacaactc
      781 acgcctgtgg cttcgagttt cccgggcacg ggctcgcgtc cgcagatcga acggcgtcgg
      841 ccagccagcg tcggacatca gcttctgttt ccagtttccc ttccagccag agccgttctg
      901 cctccgcgag aagctcaccc atggtcgcgt cgcgcggcac tcccgcctcc gcaagcacgg
      961 tgccgttcac gggcatgcgg ctcttcccgc cgtgtgcttt cccttcggcg ataatccggt
     1021 cggcgatgtc acgaattcgg gaaacggcgt ccgccaaccc gaacgacgcc gcatctgccg
     1081 cccagaacga cacgagggcg ccggttctgt cattgcgcac cagttgtgcg atgcgcccct
     1141 ctctgagaga gttctgcgcc gcccagacca tgcgccgggc aatcagcgcg ccgaccccat
     1201 ccaccaccga ccgcggcgcg cgaagtgacg ccagcacggc ctcggcgcgt gaggctgcct
     1261 cttcgtcgta cggctcgaca accctgtcgg cggattcatt cggcgccgcg cgtgctacgc
     1321 cgtctgccag cacgcacgcg agaaccagca caatcgatgc atccgaggcc agcgcctcaa
     1381 ggcgcgccga agtgcggaca atcgtttccg acgaccggac gacatcgggc ccgaaaacgt
     1441 gtggtagaag cccgagttcg gcaagaagac ggaatgccac ttcgcggtgt tcctccgtca
     1501 tcatgaattc gagttcctgc cagatccgct cccgcgcgat tctgccaagg gatggagcgg
     1561 ccgacatgag ggcgagccgg gtggtttctt ccagcgaaaa cccgaatctc gccgcaaatc
     1621 tcacggcgcg gatgacgcgc aacgcatcct ccccgaatcg ctccgccggg tccccgattg
     1681 ttctgatgat gccttccctg aggtccgcga gccccccgca aagatcgatc acggaactcc
     1741 tggacaggtc aaatgccatc ccgttgatcg tgaaatcgcg cctgcggacg tccgcttcaa
     1801 gatcggcgta ttccacccac gaaggtcgtc gaccgtcttc gtagttgcct tccctgcgga
     1861 aggtggccac atcaaacgag agaccgtttg ccagcacggt gatgacgccg aattgtgcgc
     1921 cgagccgcaa cgatcgcgga aagagcgcct gcgtttcctc cggtttcgcg gatgtgacaa
     1981 tgtcgaaatc cttcggaatc agcccgagca ccatatcacg cacgcagccg ccaatcaggt
     2041 acgcgtcgta gcctgccgcg tgaagggtgc ggattacctc agcggcggcg gcaaactcgt
     2101 gggaggagga aaggggcggg gatatcacaa gggggagctg ttccacgagg tgccccacca
     2161 ttgcggcgtc agaacagaat cgtgcggagt tcgccccgga tcggtttccg tctgcgacga
     2221 aactccaagc gccgccgcaa gtaccgactc gagatcagat ttgtcgttgt tcaggaggga
     2281 aataacaaaa tctgcaccgc aggacagtcc ctcggcggcg aggtcgggca gttccccgat
     2341 gctttccagt acacacggga cattccgcag ccgcgtattc gacttcaccc ggcggatcaa
     2401 ctgggtgccg ggtgtttttg gcatatgatg attggcaagc agaaggtcca cgcgggaagc
     2461 gccgagtatt cgccacgctt cctcgccatc cggtgccgta agcacgtgaa ggcggaaggg
     2521 aagatgaacg aaccggttca gccagatggc cagaatcgcc cgcatggacg ggttgtcgtc
     2581 gacgacaagg atggttttgt gtctggccac agcatgcacc cgcggcgttc gggacgatct
     2641 gtcattggtg ggagtgtgct ccagttcaca aagatatgcg gtttgtgaaa tctggcaagg
     2701 ccgcttcgtg cctcccatct cattttgtcc ggggcggggc aataacgata ctatttcgaa
     2761 aggcttcttc atcaccgcag ggaggtggac aatgccgaca ctgcacgggc gcatcactga
     2821 cgcggcgaat ggcgaactcc gcgaagcgaa agtcagcgtg gtcgattgcg aggggggagt
     2881 acgcacaccg gagggggcgg ttctcaaggt ggggaacggg ctggtttcgt tctactgcgg
     2941 cggggagttc tccgttcccg tgaagggcgg cccctgccgc attgttgtgg aacgcggtac
     3001 tgagtacgag ccgctggtga aacacctcaa cgtcccctgg aaaggcgacc tcgacgtcac
     3061 tctggagctg cggcgatggg ttgatctccc ccgcatgggc tggtacccgg gcaacacgca
     3121 catccactac gatcagaacg aaagacagcc gtacgaacgg cttcgccttg aaccacacgt
     3181 gaatgacttc gccgtcacgg tcgtaagcat tctccagcgg tgggatctcc cgtacgcatc
     3241 caaccgtttt cccctcggcg cgttcaccga gttcagcacg gcacatcatg tggtggatgt
     3301 gggagaagag gtccggcata acttctggga gggagggttc ggatacggcc atctgctggt
     3361 gttccgtatc cacgaccagg tggagccgtt gagccggggg atgctggtca acgatttcaa
     3421 ccccgactat cccccgctct gcttggcgtg cgacgacgcg caccggcagg gcggaatagt
     3481 ggtgtggtgc cacaacggcc tcggaatgga agccccagtg gcagccgcgc tgggcaagct
     3541 ggacgcgctg aacctttttg atccctactg gatgaacccg gagtacgact tgtggtacaa
     3601 aatgctcaac tgcggattcc atctgccggc gagcaccgga agcgactggt tcgtctgctc
     3661 aaataaccgc gtatacgcct gcaccgggac agatttttca tacgaaacct ggttggcggc
     3721 actgaagaag ggtcagacat tcattacgaa cggcccggcg ttgcttcttt ccgtggatgg
     3781 tcacgggccg ggcgcgtttc tgccctgcac ggcaaaacgt gccatcgagg tgaaagccga
     3841 gtggcgttct ttccacccga ttgaggttgt cgagatagtc cgcgacggtt tcgtgattgc
     3901 gcggaaggag ttcgcaaccg ggagccggga gggatcgttt gttcagacag tgctcccgga
     3961 aaccgacggg tggattgccg cccgctgctc aggcaatgcc cgcgacagct tcgatcaggc
     4021 catcttcgcg catacgagcc cggtctacgt ccagaccggc gccctttccc cggcacggga
     4081 agaagccgcc cggttctttg cagatgtgat ccgtggctca gttgactggg tcaacacgaa
     4141 agggcgcttc cacaacaagc agcagcgcac caccgttctg aaccttttcc gggaaggggc
     4201 ggccgtctac gaaggcatgc tccgaaaggc gcacacgtaa atacgaactc tgacgaagcg
     4261 gcttcaaagg ctctattcgg tggttcggat gacacggaaa acctgcaccc ggttgtttcc
     4321 cgtatcggcc acccacacaa cgttgccggc agggtcactt gccacgctgc agggctccag
     4381 aaactcgcca tcttcgtttc cgcgcaaccc atgttcacct gtaaggtgat acatccggtt
     4441 gaggaacacg agccggttgt tcccggtgtc cgcaatcaat atgtccccct gtggagtcac
     4501 gttcacgccg cggggacgca gaagaatccc ctctccccag gtgcgcagaa gcgtcccgaa
     4561 gcggtcaaac actgcgatcc tgtcgttccc ggtatcggca accacgatgt tcccgtcggg
     4621 gctgcacgcc accccggcgg ggtcggagag aatgccggtt gactggatcc tcgctcccaa
     4681 ttctgagggg gtttcctggt agagctggat aagtcggtct gcatcgatgt cggtgcacca
     4741 gatactggca tcaacgttga gcgcgatacc gttcggatac ccgagcgggc cgccttccgc
     4801 gtccctgttg atcacgcccg cccagacgag gtcctggccc aacctcacca gacgccggtt
     4861 atacgtgtcg gccacccaga tatccagatt gcggcccgct gcgatgcctg ccgggcggtt
     4921 gaactggccg tcgctccacc cgaacccgcc aatctcgcga acataacgcc cctcgccgtc
     4981 gaactcctgt atgcggtcgt tccctgtatc agcgacgtac cagtgcccga atgacgcgca
     5041 cgcgatgccc tgcggttgac ggaagcaccc ggatgacgaa ccgcgttcgc cgaacgagcg
     5101 catgaaaaca aggcgcaccg ctcgcgtcgg cgcgagtgcg ctgagaccag attcgagctg
     5161 gtatcctccg cacccgcaga acagcagcgc catcacagtg aggagacgca accgcgatgc
     5221 agtcattctc gttcctcgcc tcgggcgtgg gcttgatcca gcccccgccg ccatgccgac
     5281 gctatcagat agtcgaaaac ccacatcggc cagcgcgcgc cgaggtgtcg cttcacgagt
     5341 gcttccacac gacgcgaaac acatggcgtc gtgacaaacc ggatcgcgct gcgcttccaa
     5401 cggttccttt ttgcgtagcg aaccggaccg agcagatctg cgcggagctg cgtcgtgaaa
     5461 acagggtctt cgtcaaaaag aacaggcatg cactccctgg cgaacgattc ggacagggaa
     5521 agcacatcct ccaggcgccg cctgtgagcg tgatacgcgc gcgcctgcgg cacacggacg
     5581 aaccgcgctc cgtgtttctg caaccgccac gccagttcta tgtcttcacc tccccaggcg
     5641 cgcactcgtt cactgaagcc ccccgcttct cgcaaaagaa acgtcggcac ggcggagttg
     5701 taggtcagaa aatacctgaa tgggatcggt tcatcggctt tcagttttgc gatgccgcgc
     5761 gtttcgaggt aaagtgtcag tgcgtcgcgc ggaatgtcgg ggtggaaaca gacgcttccc
     5821 aacccgaccg cccgccccgc gggatcaatt gcctcctggt aagcgcgtat gaaacccgga
     5881 aggaccctca tgtcatcatc gagaaaaacc accagcgggc tgtccaccac agccagcccg
     5941 gcatttctcg cggcggaccg cccgcagttc ttctccagtc ggaggaaaat cagtcggaac
     6001 gggaacgctc cgggatcaag ctcaactgct ggaaccgacc cgtcatcgac cacaacgacc
     6061 gaaaacggca cttctctctc gaattgcccg gcgagtgttt ctagtgctgc ggaaagatgc
     6121 tccggccggt tgtaggttgg catgagaacg gttatctcag ccatcgttcc tgttctccgg
     6181 ggaggagttg cgggcggaaa gcctcgcata tacctcaagc aaacggtcgg attcgcgctc
     6241 ccacgtaagc tcgcgcgccg ctttcacgca gttttcggac aatcgttttc tgtcggagac
     6301 ggccctccgt atcgcgttcg caaccgcctc aaggtcgcat cccggctcaa ccagtacacc
     6361 gacgtcgtac tcgcgaacta cccgtcgaat ttccggccaa ccggtcgcca ccacagggac
     6421 tcccgccgcg atgtactcga acagcttgtt gggcagagca agctgcaggt tcattccggc
     6481 ggattcgcca aggaacagcc cgatgtcgca cgcagccgtg tacggaggca aatctttgaa
     6541 cggaacggca tcaagaaaac tgatccgatc cctggctggc gacgcggcgg cgcggtgctc
     6601 catgtctttt ctcagcggcc ccgcacctat gagaacaagg tgtgcgtccg gaagaagcgt
     6661 catggtgtca atcgcccgtt ctatgcccag ccccggaagg aaaccaccct ggtagagaac
     6721 aagcacacgc gaagggggac agccagtcat ctcgcgaatt ggtgccggtt cgacgggacc
     6781 gatctccttt ggcagattgc gcagcacgac cgcgccgaca ccatatcggg cgcgcagttc
     6841 gtccgcaata ccctcacaca ccgttatcac ggcatcactt cgggggatca gcgtttttgc
     6901 gaaaccctgc cacgcggcgc gaacgactgg tctgcccacg agactgggaa gctcaaccca
     6961 gagttcatgg gcgtcgtgca cgagctgtgc gcgcgtcagc gatgccgcca accaggctgg
     7021 aaacagcgtg tcaaggtcat gagaatgaat gatccgggct ctgcgctcca tcaacgctga
     7081 gaacgcgcgc cgccagaaac acgcgtacct gatgcgcagg gagtccgatg ttcccaccgg
     7141 gattcttttg acagttacct cttcccagcc ggtcaacggc ggagtgctcg gtgtttccat
     7201 cccgatgacg cggacgttgt agccggcggc gatgagcgac gttgcctcct tgaagacacg
     7261 cgcgtcgtgc ctgaggtcgt tgtacacaac catgtcgact gcgatgccgc cgccgttacg
     7321 cgcgttcatc tcgacccctt cggaagcaat gcacggaact tgcgcttcca tgcgggtgcg
     7381 gttttcgcaa caaaagaagc ccggcgagac cgcgaccatt ccacggcgag gtagggtgtc
     7441 agggttcccc cgaaaccgcg tttgtaatgc gcaatcgtct ccatgttcgc tccggcgaag
     7501 tcgaactcgc ccaccttccc tgaagcgaat tcaaacgcac tgcagagggc caatgcgctg
     7561 gctcccacag gtatgccttc gtcagtagtt cccgcgatga gttcatacgc tgattggccg
     7621 gccccggtgg cagtgggcgg gtcgaacccg attgcgatgg tggcaagaac tgcatcgccg
     7681 cggaaaaccc ccatcgtggc aacattgttg cacaccgaca ggagtgcgag gtagtcgtcc
     7741 caggcttgtg cgggaagggg aggggcttct ccctgccgcc ggaatgttgc tgccactgtt
     7801 tccctcaatg ctccgtgcgc atgttcgccg gtgagacact ccgttctgag attcgcctcg
     7861 cgtg
//
LOCUS       DAOTNG010000137         7829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8235_length_7829_cov_4.687420, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000137 DAOTNG010000000
VERSION     DAOTNG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_7829_cov_4.687420"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..2981)
                     /gene="ppdK"
                     /locus_tag="P5179_07845"
     CDS             complement(276..2981)
                     /gene="ppdK"
                     /locus_tag="P5179_07845"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRS95167.1"
                     /translation="MADAKKYVYLFGGGTAEGNAKMKNLLGGKGANLAEMAAIGLPVP
                     PGFTITTEMCTVYYKLNQRYPAELDEQVKTGIAHIEKIMGKQFGSPTNPLLVSVRSGA
                     RISMPGMMDTVLNLGLNDTTVKGLIKQSGNERFAWDSYRRFVQMYGDVVLGLKPQSKD
                     EEDPFEVIIKAKKAEKGVKNDTELTTEDLKDLVVKFKAAVKQRKGMDFPEDPKEQLWG
                     AINAVFGSWMGNRAIIYRRINKIPEDWGTAVNVQAMVFGNMGEDSATGVAFTRDPATG
                     EKVFYGEFLINAQGEDVVSGVRTPSPIAELAKTQKAAYEQLEDIRAKLEKHYKDMQDI
                     EFTIERNRLFMLQTRNGKRTAQAAVRIAVDMVKEKLITKEAAVARIDPDQLDQLLHPM
                     FDPAAKKKATVLAKGLNASPGAAVGKVYFNAEDAEAAAGRGEKVILVRLETSPEDIGG
                     MAASQGILTVHGGKTSHAAVVGRGMGKPCVVGCGALSVSYTERRFNVTVGGQTITVKE
                     GDVISIDGSDGSVMLGAVPTKDPEITGDFSMLMKWVDEIRTMGVRTNADTPHDAKVAL
                     DFGAEGIGLCRTEHMFFGGDRIDAVREMILADDLAGREKALAKILPMQQEDFVGIFRV
                     MNGLPVTIRTLDPPLHEFLPNTDDQIEELAYKINRNPMRIREKLHQLHELNPMLGHRG
                     CRLGMVYPEITAMQARAIMQAACQVKAEGIDVHPEIMIPLVGTMGEMTNQKDLVVKVA
                     KQVMKETGVTVNYMVGTMIEVPRAAIIADKIAKEAEFFSFGTNDLTQMTCGYSRDDAG
                     KFLPFYIENKVWIDDPFQSLDQEGVGKLVEMGVKLGRKGRKNLKIGICGEHGGDPASV
                     QFCFRVGMNYVSCSPFRVPIARLAAAQAALAAKDASTAGASKSTM"
     gene            complement(3222..4115)
                     /gene="era"
                     /locus_tag="P5179_07850"
     CDS             complement(3222..4115)
                     /gene="era"
                     /locus_tag="P5179_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRS95168.1"
                     /translation="MEQNTTGTTRCGYIAIIGPPNAGKSTLMNALVGERLSIVTPKPH
                     TTRTRVLGIITDPNRNAQAIFLDTPGMVAEPRYRLQEAMMEHIHRSIRDADVILAMFD
                     YTRPDPVLIQRTVELAAVPDKAAVFVLNKVDLAEKEPDLSPYPEGTIPVSALLELNLR
                     PVGDAITAHLPEGPFLYPPDTIAEQPERFFVAELIRETIFVTMKEEIPYTTAVVVEEF
                     IERTPKDYINATILVDRDSQKGIMIGRKGQQLKQIGQLSRKKIEDFLGRPVYLELFVK
                     VKRDWMKKDSELRDLGYLERK"
     gene            4307..5383
                     /locus_tag="P5179_07855"
     CDS             4307..5383
                     /locus_tag="P5179_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95169.1"
                     /translation="MAARYRVAIVGCGGIAHTHADSCRKMPEVDIVGLADVRPEALAE
                     FGKTFEVPAERHFTDYLTMYDAVKPDIVCVCTRPAQHCAPTVAALQRGIHVLCEKPMA
                     LDLEEADAMVAASEQSGAKLAINTQRHTDPVYLYAVKLVKEGRIGELRTLRSECKTYE
                     AGYGMMNIGAHYLDAMRLFGGDAAWVFAHLTNAAGNDITPADIEPGERDTGLTAGKAC
                     TMQIGYASGVMGVSEYWVGTATFGFEIVGTKGALAIRGRDASIFVTNGGGGKANDEIV
                     WETIPVPLADEEKEVVAERRWSTIFMMRALIKGIEENAVPACSGYDGRAVLEQIMATY
                     VSQISGSRAKLPLADRRHPLKHWQ"
     gene            5442..6677
                     /locus_tag="P5179_07860"
     CDS             5442..6677
                     /locus_tag="P5179_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRS95170.1"
                     /translation="MNTAGFLDDIRSFRGFGRETEVRETRSASFSGEPVSVATFVNEF
                     WTSRQRAAHSLHELSYRACFKPQLPRFFIERLTAPGDHVYDPFMGRGTTPLEAALLGR
                     VPLGCDVNPLSEMLVRPRLHPPQMDEIEQRLAEIDLDAATAVRTDLKVFYHPETLREV
                     CALRHYLHAREQRGELDALDRWIRMVAVNRLTGHSPGFFSVYTLPPNQAVTVAQQRKI
                     NLRRNQKPPRRDVREIILRKTRSLLRDCDDETRRVLANAGKDARFLTQPAGSVPGIPG
                     DSVRLVVTSPPFLDIVNYAQDNWLRCWFCGIDAGSVGITMARKLEEWQAAMSEVFREL
                     ARVLTPSGHVAFEVGEVRTGTVNLEEAVIPCGIAAGLSPVCVLINDQHFTKTSNCWGV
                     ENRAKGTNTNRVVVFRKER"
     gene            6680..6814
                     /locus_tag="P5179_07865"
     CDS             6680..6814
                     /locus_tag="P5179_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95171.1"
                     /translation="MNHEPHETHEKNAERAFVTLMTTVPLTVIPAKAGIQNTKPYNVA
                     "
     gene            complement(7097..>7829)
                     /locus_tag="P5179_07870"
     CDS             complement(7097..>7829)
                     /locus_tag="P5179_07870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS95172.1"
                     /translation="TRIPPDQTPKESDAYRTGFAYSTDGGRSWGEPVFAPAGEPWSHY
                     SPFGRILTVRDGRLALPVYLAKKTWLIWSSDNGRTWEDLTLVAEDINETAYCVLPSGE
                     WVLVGRENNGTGMFSLVRWSQDAGRTWSAPVQFLENRRLPSDLVVLSDGSLLAVHGYR
                     TIPRGVRAVRSTDGGRTWGSTDLVLLEGKPNTDLGYPTVEVKDGWVVIAYYDASAAPE
                     DKTDPTGAYLEVVRVREQEIIERVR"
BASE COUNT         1449 a         2312 c         2445 g         1623 t
ORIGIN      
        1 aaacgtcgtc ttcgtgtcaa gctttttcgc gaagaagttg caaatttgca ccagggtttc
       61 tgggagggtg tgcacagggc cgcacagaac acgaaatgtg accgcgccgg ggggatgggt
      121 cgcatgggac taatggactg atagatcgcc gttccgcggt ggatcgggca aaaaaccacg
      181 aagccgcacc cgaggatcgc tcaggtgcgg cttcgcttat tgtgtgcagg gcgcaagttc
      241 tctgtaagac cgcttacctg cacaggccgt gtgatttaca tggttgactt gctcgcgcct
      301 gcggtggagg catccttcgc cgccaacgca gcctgcgccg ccgccagccg ggcgatcggc
      361 acgcggaacg gcgagcacga aacgtagttc atgccgacgc ggaagcagaa ctgcaccgat
      421 gccgggtcgc cgccatgttc gccgcagata ccgattttga ggttcttgcg gcccttacgg
      481 ccaagcttca cgcccatctc gacgagtttg cccacgcctt cctgatcgag cgactggaac
      541 ggatcgtcaa tccagacctt gttctcgatg tagaacggca ggaacttgcc ggcgtcgtca
      601 cggctgtagc cgcaggtcat ctgggtgagg tcgttcgttc cgaagctgaa gaactcggct
      661 tccttcgcga tcttgtcggc gatgatggcg gcccgcggaa cttcgatcat cgtgccgacc
      721 atgtagttca ccgtcacgcc ggtttccttc atcacctgct tggcgacctt cacgacgagg
      781 tctttctggt tcgtcatttc acccatggtg ccgacaagcg ggatcatgat ttccggatgg
      841 acgtcgatgc cttccgcttt cacctggcag gcggcctgca tgatggcgcg ggcctgcatg
      901 gcggtgattt ccggatacac catcccgaga cggcaaccgc ggtggccgag catggggttc
      961 agctcgtgca actggtggag cttttcccgg atgcgcatcg ggttgcggtt gattttgtag
     1021 gcgagttcct cgatctgatc gtcggtgttc ggcaggaact cgtgaagcgg cggatccagc
     1081 gtgcggatgg tgacgggcag gccgttcatc acgcggaaga tgccgacgaa gtcttcctgc
     1141 tgcatgggca gaatcttcgc cagcgccttt tcgcggcccg cgaggtcgtc cgcgagaatc
     1201 atttcgcgca ctgcgtcaat gcggtcgccg ccgaagaaca tgtgctcggt gcggcagagg
     1261 ccgataccct cggcgccgaa atcgagcgct accttggcgt cgtgcggggt gtcagcattg
     1321 gtgcggacgc ccatggtgcg gatttcatca acccacttca tcaacatgct gaagtcgccc
     1381 gtgatttccg ggtccttcgt cggaacggct cccagcatga cggaaccgtc ggaaccgtca
     1441 atcgagatca cgtcgccttc tttgacggtg atcgtctgtc cgccgaccgt cacgttgaac
     1501 cgacgttcgg tgtagctcac gctcagcgcg ccgcaaccga cgacgcaggg cttgcccatg
     1561 ccacggccga caacggcggc gtgggaggtt ttcccaccgt gcaccgtcag gattccctgc
     1621 gaagccgcca taccgccgat gtcctcgggg ctggtctcga gacggacgag gatgaccttc
     1681 tcaccgcgtc ccgcggcggc ttccgcgtct tccgcgttga agtacacctt gccgactgcc
     1741 gcgccggggg aggcgttcag gcccttcgcc agaacggtgg ccttcttctt cgccgccggg
     1801 tcgaacatcg ggtgaagcaa ctggtcgagc tgatcgggat caatgcgggc gacggccgct
     1861 tctttggtga tgagcttctc tttcaccatg tcaaccgcga ttcggacggc cgcctgcgcg
     1921 gtgcgtttgc cattgcgcgt ctggagcatg aacaggcggt tccgctcaat ggtgaactcg
     1981 atatcctgca tgtccttgta gtgcttctcg agtttcgcgc ggatgtcctc aagctgctcg
     2041 tatgcggctt tctgggtttt cgccagttcc gcgatcgggg agggcgtgcg cacgccggaa
     2101 acgacgtctt cgccctgcgc gttgatcagg aactctccgt agaaaacctt ctcaccggtg
     2161 gcgggatcac gcgtgaaggc aacgccggtg gcggaatctt cacccatgtt gccgaacacc
     2221 atggcctgca cgttaacggc ggtgccccag tcttcaggga ttttgttaat gcggcggtag
     2281 atgatggcgc ggttccccat ccacgacccg aacacggcgt tgatggctcc ccagagctgc
     2341 tccttggggt cttccgggaa gtccatgcct ttgcgctgct tcacggcggc tttgaacttc
     2401 accacgaggt ccttgagatc ctcggtcgtc agctcggtgt cgttcttgac acctttttcc
     2461 gccttcttgg ctttgatgat gacttcgaag ggatcctctt cgtctttgga ctgcggcttg
     2521 aggccgagca cgacgtcgcc gtacatctgc acgaagcggc ggtagctatc ccacgcgaat
     2581 cgctcgtttc cagactgctt gatgaggcct ttgacggttg tgtcattcag gccaaggttg
     2641 agcaccgtat ccatcattcc aggcatggag atacgcgcgc cggagcggac ggacaccaga
     2701 aggggattcg tcgggctgcc gaactgcttc cccatgatct tttcgatgtg cgcgataccg
     2761 gtttttacct gttcgtccaa ttccgcgggg taccgttggt tcagcttgta gtaaacggtg
     2821 cacatctcgg ttgtgatggt gaaaccggga ggtacgggga gaccaatcgc cgccatttcg
     2881 gcgaggttgg cgcccttacc gccaagcagg ttcttcatct tcgcgttccc ttcagcggtt
     2941 ccaccgccga acagatacac gtacttcttt gcgtctgcca tgaagttgcc tccgtggaat
     3001 tgttgaccga aaggtgaatt gtgcgcttgt attcagttcc agtgattgtg cgttccgggt
     3061 atggtccaga tggcgggttg agcgccttgc tcacgggtaa ccgggtcggg tgcgctgcaa
     3121 aaccactaga atgcaaaaat ataccgaaat cgtgagtgtt tcggcaagta tccgcgcggc
     3181 gtgtgaggtc aaaatggcag gaattggtcg ggagggatgc cctatttccg ttccagatac
     3241 cccagatcgc gaagttcgct gtccttcttc atccagtcgc gcttcacctt cacgaaaagt
     3301 tccagataga ccgggcgacc caggaaatcc tcgatcttct tccgggaaag ctgaccgatc
     3361 tgtttgagct gctgtccctt cctgccgatc atgatgcctt tctgggaatc ccggtccaca
     3421 agaatggtcg cgtttatgta gtcttttggc gtgcgttcga tgaattcctc caccaccacg
     3481 gccgtggtgt agggtatttc ttccttcatc gtgacaaaaa tcgtttcacg tatgagctcg
     3541 gcgacgaaaa accgctccgg ttgctcagcg atggtatcgg gcgggtagag gaacggaccc
     3601 tcagggaggt gtgccgtaat ggcatcaccg acgggacgga ggttcagctc gaggagggcg
     3661 gagactggaa ttgtcccctc cgggtacggc gaaagatccg gttctttctc cgcaaggtcc
     3721 accttgttga gcacgaacac cgctgccttg tccggcacgg cggcaagctc caccgtgcgc
     3781 tggataagca ccgggtccgg tcgcgtatag tcgaacatgg cgagtatcac gtccgcgtcg
     3841 cgtatggagc ggtgaatatg ctccatcatc gcctcctgca ggcggtagcg tggctccgca
     3901 accattccgg gtgtatcgag gaaaatggcc tgcgcgttgc ggttggggtc cgtgatgatg
     3961 ccgagaacgc gggtgcgggt tgtgtggggc ttcggggtga cgatgctcag tcgttcgccg
     4021 acgagggcat tcatgagggt cgatttgccc gcgttcggcg gaccgatgat cgcgatgtat
     4081 ccgcatcggg tggtgccagt ggtgttctgt tccatcgtgt gttccgtcgc cgtgtattga
     4141 ggggtgcaat gtcgggacag gtggttggaa gaaagataca gaccggcaac gattacggcg
     4201 cccctcgcaa aacatgccag ccatctttgt ccgcgcttcc ggcggtcgct acattcacgt
     4261 tatcggcctg ttttccgtgt taactggttt ctttcaggag gcattcatgg cggcgcgtta
     4321 ccgggttgcg atcgtagggt gcggcgggat agcgcatacc cacgcggatt cgtgcaggaa
     4381 gatgccggaa gtagatattg tgggtctggc ggatgttcgt ccggaagcgc tggcggagtt
     4441 cgggaaaacg ttcgaagtcc ccgcggaacg gcatttcacc gattacctga cgatgtacga
     4501 cgcggtcaaa ccggacatcg tgtgcgtgtg cacgcggccc gcccagcatt gcgcgccgac
     4561 ggtagccgcg ctccagcgtg ggattcacgt gctttgcgag aagccgatgg cgctcgacct
     4621 cgaagaggca gacgcaatgg tggctgcaag cgaacaatcc ggagcgaaac tcgccattaa
     4681 cacgcagcgg cacaccgacc cggtctacct ttacgcggtg aagcttgtga aagaaggccg
     4741 catcggggaa ttgcgcaccc tcagaagtga gtgcaagacg tacgaagcgg gctacgggat
     4801 gatgaacatc ggtgcgcatt acctcgacgc catgcgtctg tttggcggtg acgcggcatg
     4861 ggtgttcgcg catctcacca acgcagcggg gaacgacatc accccggcgg acatcgaacc
     4921 gggcgagcgg gataccggcc tcaccgccgg aaaagcgtgc accatgcaga tcgggtacgc
     4981 cagcggggta atgggcgtgt cagaatactg ggtcggcact gcgaccttcg gcttcgaaat
     5041 cgtgggaacg aaaggtgcgc ttgccatccg gggccgggac gccagcatct ttgtaacgaa
     5101 cggcggcggc gggaaggcca acgacgagat cgtctgggaa accatccccg tgccgcttgc
     5161 cgacgaggag aaggaagtcg ttgcggagcg acgctggagc accattttca tgatgcgcgc
     5221 ccttatcaag gggatcgaag agaatgcggt gcccgcctgt tcggggtacg acgggcgcgc
     5281 ggttctggaa cagatcatgg cgacgtacgt atcgcagata tcaggctcgc gggcgaaact
     5341 gcctcttgcg gaccgccgcc atccattgaa gcactggcag taggcatacg ggagccacaa
     5401 cccctccata gccgccgaag gacgtaacgc tgccggatgt gatgaatact gccggatttc
     5461 tggacgacat tcgcagcttc cgcggtttcg ggcgcgagac agaagttaga gagacccgtt
     5521 ccgcgagctt ctcgggggaa cccgtcagcg ttgcgacgtt tgtgaacgag ttctggactt
     5581 cccgccagcg cgccgcgcac agcctgcacg agctttccta ccgcgcctgt ttcaagccac
     5641 agttgccgcg ctttttcatc gaacgcctga ccgcgccggg tgaccacgtg tacgatccgt
     5701 tcatggggcg cggaacaacg ccgcttgaag ccgcgttgct gggccgggtg ccgctggggt
     5761 gcgacgtgaa tccactcagc gaaatgcttg tccgcccgcg cctgcatccg ccgcagatgg
     5821 acgaaatcga acagcgtctg gccgagattg acctggatgc ggcgaccgcc gtgcgcaccg
     5881 acctgaaggt gttctatcac ccggaaacgc tgcgcgaagt ctgtgcgctg cggcactacc
     5941 tgcatgcccg ggagcaacgt ggagaactcg atgcgctcga ccggtggatc cgcatggtgg
     6001 cggtgaaccg tctcaccggt cactcgccgg ggtttttctc cgtatacacg ctgccgccga
     6061 atcaggcagt aactgttgca caacagcgga agatcaacct gcgccggaat cagaagcctc
     6121 cgcggcgcga tgtgagggag atcatcctga gaaaaaccag gtccctgctg cgggattgcg
     6181 acgacgagac acggcgagtg ctcgcgaacg ccggaaagga tgcgcggttt ctgacgcaac
     6241 ctgccgggtc ggttcccgga atacccggcg attccgtacg actggtggtc acctcaccac
     6301 cgtttctgga cattgtgaat tacgctcagg ataactggct tcggtgctgg ttctgtggga
     6361 tcgacgcagg cagcgtcggt attaccatgg cgcgcaagct ggaggagtgg caggcggcga
     6421 tgagcgaggt gttccgtgag cttgcccgcg tgctcacacc cagtgggcac gtagcctttg
     6481 aggtgggtga ggtgcggaca ggaacggtga acctcgagga ggcggtgatt ccgtgcggca
     6541 tcgcggcggg cctctcaccg gtgtgtgtgc tgatcaacga ccagcacttc acgaaaacgt
     6601 ccaactgctg gggagttgag aaccgcgcca aggggacgaa caccaaccgg gtggtcgtgt
     6661 tcagaaagga gcgctaggaa tgaaccacga accacacgaa acacacgaaa agaacgccga
     6721 gagagccttc gttactttga tgacgacagt acccttgacc gtcattcccg cgaaggcggg
     6781 aatccagaac acgaaacctt acaacgtggc ctgaggtgac gtcctgcaga agccggctga
     6841 gttgctgtcg atgtgaggca caccatcggt gccgccgaga ataaattccc gccttcgcgg
     6901 gaatgacgcg gatcagttgc gagtcccctg ttcgtagttg cacgccgcgt tcgccactcg
     6961 atgaccgttc ctccaccgtg cgacggctca acgctcgagg gacccgctcc gtcgggccct
     7021 cgacaggctc gcgcaggctc agccaccgtt tttcgtgtca cttcgtgtat ttcgtggtta
     7081 ccccatcgtt tccgcctcac ctcacccgct cgatgatctc ctgctcgcga acccgcacga
     7141 cctccagata cgcgccggtc gggtcggtct tgtcctcggg cgcagcactg gcatcatagt
     7201 aggcgatcac gacccagccg tccttcacct ccaccgtcgg gtagccgagg tccgtgttcg
     7261 gtttgccttc cagcagcacg agatcggtgg aaccccacgt ccgcccgccg tcggtggaac
     7321 ggactgcgcg cacaccgcgc gggatggtgc ggtagccgtg caccgcgagc agcgatccgt
     7381 cggacagcac gacgaggtcg ctgggcaggc gccggttttc gaggaactgc accggggccg
     7441 accatgtccg accggcatcc tgcgaccatc ggacgaggga gaacataccg gttccgttgt
     7501 tctccctgcc caccagcacc cattcaccgg atggcaaaac gcagtacgcc gtttcgttga
     7561 tgtcctccgc gacgagggtg aggtcctccc acgtgcgccc gttgtcgctc gaccagataa
     7621 gccacgtctt cttcgccaga tacacgggaa gcgcaagccg cccgtcccta accgtcagga
     7681 ttcggccaaa cggcgagtag tgcgaccacg gctcacccgc cggtgcgaac accggctctc
     7741 cccaggatcg ccctccgtcc gtcgaatagg cgaacccggt gcggtacgcg tccgattcct
     7801 ttggtgtctg gtcaggcgga atgcgcgtg
//
LOCUS       DAOTNG010000138         7797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8278_length_7797_cov_5.036166, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000138 DAOTNG010000000
VERSION     DAOTNG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_7797_cov_5.036166"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="P5179_07875"
     CDS             <1..486
                     /locus_tag="P5179_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRS95173.1"
                     /translation="AFVLFSRMAGAKQVILAGRTESSIRRAAELCHPDTSVNVTDPHF
                     VARVHEITNGEGVDRAIEGVGDTSVIDLAVPCLSPEGQIGLYGVAPSTQAKALHANHP
                     RVRAISPDESEVHEEFFSLVREGRIDAFAFLSHRLPIRDIGRGFSLLANREAFKVGLD
                     W"
     gene            500..1492
                     /locus_tag="P5179_07880"
     CDS             500..1492
                     /locus_tag="P5179_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRS95174.1"
                     /translation="MARTFTAVVFPEANRAELREFPLRAPRKGEVLARVVVSGVSIGT
                     EYLVLSGQFPNQKFPCLVGYQSVGVVEEVGEGISHLRPGDRITIGSSEIPEGYHEGAG
                     AAHFSHAVIPAEPGNDRAIIVPSHVSDDAASYFVLVSVAVEGIRLANVQRNDLVAVVG
                     QGVLGQLVAQICRHNGATVFTSDLDPARVEISARYSAHRAFALDVPAFDEELRKVQKK
                     GADVVFETTGNTKVFDQALTLARDYGTFVAQGHYPGNLTFRFVHAHWRHLRMMFPCGK
                     GKTWESVETMARGIVNVDSLITHRVSPQDAPDLYAKVAARSPEILGATIRWAER"
     gene            complement(1619..2767)
                     /locus_tag="P5179_07885"
     CDS             complement(1619..2767)
                     /locus_tag="P5179_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS95175.1"
                     /translation="MAVVDIRAQAKRRTPEELIRHLQTFSLELNFAAGVWFFAPGGGR
                     FHDRYTPNLSIPERLKLAGELAPYGLKGVEAHYPTEVNEGNWHHYQQLEKDTGIKLVG
                     FGPALFYDKDFEWGALSSPYPEVQEKAIDIFARALKFAKDTGTPHVIHWPGIDGWENN
                     FGIDFIAMRDRFARNLAKAMDRVPGVRVCMEPKPYEPRGRIIYGSTYEGLLLAQKVES
                     LLEHPENKAILAEGHALVAMNPEVGHMLMGFEDLAYSFSLIIEYGKLAHTHWNSQPLG
                     NYDQDMNVGVLSPEQTEAALYVLKMHGYKGYFSPDLNPERMSPLQALKNCFDALRAAN
                     ERIDNLDHEKVIMSTTFPRKNRGWLEAYLIRQRAGDVAGLSEMPEFEK"
     gene            complement(3186..3758)
                     /locus_tag="P5179_07890"
     CDS             complement(3186..3758)
                     /locus_tag="P5179_07890"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRS95176.1"
                     /translation="MEPDIRAAKTVLRAEMKARRAELSEAECEESSRRIFTRLLTLPE
                     WQNARTVHIYIGALPGEVRTLPIIRWCWDEGRNVIVPVVGKDGDMGHVFLAPATALAT
                     TRWGGLEPRDAEPADSLTADVVIVPGVAFDRSGNRLGMGGGFYDRFLSTVTTPKIALA
                     HAFQIVDAVPVNSHDRRVDLIVTPDEVIRA"
     gene            complement(3763..6321)
                     /locus_tag="P5179_07895"
     CDS             complement(3763..6321)
                     /locus_tag="P5179_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS95177.1"
                     /translation="MRSLVFCFLASWCAAPLIAHAATLSGVVIDAETGAPVTAAVIHL
                     DNTPHGTLADQNGAFTLNGLPAGRHIVTVGLIGYRLHKQRVTLGDEPVSLSIALHPEV
                     LPGQTVLVTASRVKDREAPVTFTNLPREEIARTYSVQDVPVLLQSLPSVHAYSDAGNG
                     VGYSYLSVRGFGQKRVNVLINGIPQNGPTSHEVYWVDLPDVLSNVQDIQVQRGVGSSL
                     YGSSAIGGSINLITTNFNPERSVTAFAGAGSYGTRKLSVAFNSGLVDDSYAMYGRFSR
                     ITSDGYRQQSWSDLWSYFLGVRRFDPSMTTTLLVYGGPERTHLAYNGVTRAYLDGTVS
                     GDARADRRYNPLTYENEIDTFNQPHYELHHDWQIAPALRLNTTLYAITGEGYYDQFRT
                     ARSLEEFRLPTFRVKDPSLYPLSWYTRNADGTPRADANGDYTITKSDLTRRRNVENRE
                     YGVLPRMAWEHHGGTLTAGGELRAHSGRHWGQITWLAAPPPGVAPGHLYYDYRERKLT
                     TSAFVQEMAHILPRVTLMADLQAVRHLYTLDRDSLTRYRYDAEFRWISPKAGVNVNIT
                     DRLHAFTNIAYARREPITDDLADPQDYWNEPSFAERETTFVGGQIGQIRLKNPTVKPE
                     RVLNFELGTGYRSPRINAMLTLYHMDFRNEIVPWVGQISEASGNPVTGNADRSVHQGI
                     ELEATATPAHFLELGGNVSINNDHFVRYTEHQMDWDAWQTVSLPRNGNRIAGFPTMLA
                     NARARLTHGGWSLGATWRYVGRQYLDNAENTANSIAPYHVTDVSLAYRIEKRFGLKGM
                     EVRLSVNNLFDRLYEASGYVDDVYAVASQTAIYATPYFIPAAPRNAFFSIQWEL"
     regulatory      complement(6413..6511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7037..7471
                     /locus_tag="P5179_07900"
     CDS             7037..7471
                     /locus_tag="P5179_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95178.1"
                     /translation="MRRQLIAPTRILVALLLPALCVAAEQSEGEAREAPSPPMSVMVS
                     VERTGAAAILGTNSAGSSTEFAITAKWLVLVFEADYRDMGWSRRSLVAPAGLSGSTEW
                     GSLTRLAPGIQYYQSVGRRWAIWGKFSAIAGFEETPSLRSVT"
     gene            7618..>7797
                     /locus_tag="P5179_07905"
     CDS             7618..>7797
                     /locus_tag="P5179_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95179.1"
                     /translation="MIAFPEAYARYRFNERVALRADYEWDTRVFGLAANDPTVQDGYL
                     RIRDHYPALNVEYTPA"
BASE COUNT         1348 a         2333 c         2579 g         1537 t
ORIGIN      
        1 gctttcgtgc tgttttcccg catggcgggc gcaaagcagg tgatccttgc cggccgcacg
       61 gagtcctcga tccggcgtgc ggcagagctg tgtcacccgg atacctccgt gaacgtgacc
      121 gacccgcatt tcgtcgcgcg ggttcacgag atcaccaacg gcgagggtgt tgatcgagcg
      181 attgaagggg ttggcgacac gagcgtgata gatctggcgg ttccgtgcct gtcgcccgaa
      241 ggccagatcg gattgtatgg cgtcgcaccc agcacgcagg caaaggctct ccacgcgaat
      301 catccccgcg ttcgagctat cagcccggac gaatctgagg tgcacgagga gttcttctcg
      361 cttgtgcggg aaggtcgcat tgacgccttc gcgttccttt cccaccgatt gcccatccgg
      421 gatatcggcc ggggattcag ccttcttgcg aacagggaag ccttcaaagt tggactggac
      481 tggtgaaagg ggtaaccaca tggcgcgaac attcacggcg gtcgtatttc ccgaggcgaa
      541 tcgggcggaa ctgcgcgaat ttcctcttcg cgcgccgcga aaaggcgaag ttctggctcg
      601 ggtcgtggtg tccggggtaa gtatcggcac ggaatacctc gtgctttcgg ggcagtttcc
      661 gaaccagaag ttcccgtgcc ttgtcggcta ccagagtgtg ggggtggtcg aggaagtcgg
      721 cgaaggcatc tctcatctgc ggccggggga ccggatcacc atcgggtcgt cagaaatacc
      781 ggaaggctac cacgaggggg ccggggcggc gcatttctcc cacgcggtga ttccggcgga
      841 accgggcaac gaccgggcga tcatcgtccc gtcgcacgtg tcggacgatg cggcctcgta
      901 tttcgtgctg gtgagcgtgg cggtcgaggg catccggctg gcgaacgtgc agcgcaacga
      961 tcttgtcgcc gtggtgggcc agggtgtgct cgggcagctt gtagcgcaga tctgccgcca
     1021 caacggcgcc acggtgttca cgagcgatct tgacccggcg cgcgtggaga tttccgcccg
     1081 ctacagcgcc cacagggcat ttgccctcga cgttccggcg tttgacgaag aactgcgcaa
     1141 ggtgcagaaa aaaggcgcgg acgtggtgtt cgagaccacg ggcaacacga aggtgttcga
     1201 tcaggcgctc acacttgcgc gcgattacgg cacgttcgtg gcgcaggggc attacccggg
     1261 gaatctcacg ttccggtttg tccatgcgca ctggcggcat ctccgcatga tgttcccgtg
     1321 cgggaaaggc aagacgtggg agagcgtgga aaccatggcg cgaggcatcg tcaatgtgga
     1381 ttcgctcatc acgcaccgcg tcagcccgca ggatgcgccg gacctgtacg cgaaggtggc
     1441 cgcgcggtcg ccggagattc tcggcgcgac aatccgctgg gcggagaggt aaggcttcaa
     1501 gggccgccat ttgcacagcc cggggcatcg tcccgggcgg gggaaaacaa agggacacga
     1561 cggagcgtgt ccctccacaa cgcgcatggc accctaccgc atttcagacg ggcagaacct
     1621 acttctcaaa ctccggcatc tctgacaatc cggccacatc gcccgcgcgc tggcggatga
     1681 ggtaggcttc cagccatccg cggttcttgc gcgggaaggt ggtggacatg atcaccttct
     1741 cgtggtcgag gttgtcgatc cgctcgtttg ccgcgcgcag ggcgtcgaag cagtttttca
     1801 gtgcctgcaa cggggacatg cgctcggggt tgaggtccgg gctgaagtag cccttgtacc
     1861 cgtgcatctt gagtacgtac agcgccgctt cggtctgctc gggggaaagc acgccgacgt
     1921 tcatgtcctg atcgtagttg cccagtggct ggctgttcca gtgcgtgtgc gccagcttgc
     1981 catattcgat gatcaggctg aagctgtagg cgaggtcctc gaagcccatg agcatgtgcc
     2041 ctacttccgg gttcatcgcc acaagcgcgt gcccttcggc cagaatcgcc ttgttttccg
     2101 gatgctcgag cagggactcc accttttgtg cgagcagaag gccttcatac gtggacccgt
     2161 agattatccg tccgcgtggc tcatagggct tcggttccat gcagacgcgc acaccgggca
     2221 cgcggtccat cgctttcgcg aggttgcggg cgaaccggtc gcgcatggcg ataaagtcga
     2281 tgccgaagtt gttctcccaa ccgtcaatgc cgggccagtg gatgacgtgc ggcgtgccgg
     2341 tgtctttcgc gaacttgagc gcccgggcga agatgtcaat cgccttttcc tgcacctcgg
     2401 gatacggcga cgaaagcgct ccccactcga agtctttgtc gtagaagagt gcggggccga
     2461 acccgacgag cttgatgccg gtatcctttt ccagttgctg gtagtggtgc cagttgccct
     2521 cgttcacttc ggtcgggtag tgcgcctcga cgcccttgag gccgtatggg gcgagttctc
     2581 ctgccagttt gagccgctcc ggtatgctga gattcggcgt gtaccggtcg tggaatcggc
     2641 cgccgcccgg cgcgaagaac cagacaccgg cggcaaagtt gagctcgagc gagaacgtct
     2701 gcaggtgccg gatcaattcc tccggcgtgc ggcgcttcgc ctgcgcccgg atatcaacaa
     2761 cagccatggg aatccctttc atggacgact ttggtgtgaa cgcagactca catcccatcg
     2821 taagaatatc cgtacgcagc cggtgcgggg aaagggaagg cggtcatccc ccgattggcg
     2881 cgtctctttc tcacacgacg accggttgca cttcgcgcgg tctggattcc cggctttgcg
     2941 ggaatgacgg cgtttccagg aggttgcgcg tgctgtgcgg tcgtcgagtg gcctccgcgc
     3001 aaacggaggc tgtatcgaga cgacggagga tggccttact gcgtcgacgc ggcggcgttt
     3061 cgacaggctc ccgctcgcgg aagcacgctc aacgaccgtg cgtacctcgg gtgtttgcgg
     3121 gtagctgcac cacaacgcag gggccgaccc ctgtgtcggc ccgtgtgttc tccaaaacct
     3181 gtacctcagg cgcggattac ctcgtccggg gtaacgatga ggtccacgcg ccggtcatgg
     3241 ctgttcacgg ggacggcgtc cacaatctgg aacgcgtgcg caagggcgat tttcggggtg
     3301 gtgaccgttg aaagaaaccg gtcatagaac cccccaccca tgccaaggcg gttgccggaa
     3361 cggtcgaacg cgacgccggg gacgatgacg acgtcagccg tgagggaatc cgccggctcc
     3421 gcgtctcggg gttcaaggcc tccccagcgc gtcgtggcaa gtgcggtggc gggcgccagg
     3481 aacacgtgcc ccatgtcccc gtctttgccg accaccggca caatcacgtt tcgcccttcg
     3541 tcccagcacc accggatgat cggcagcgtg cgcacttcgc ccggcagcgc accgatgtag
     3601 atgtgcaccg tccgcgcgtt ctgccactcg gggagggtca ggaggcgcgt gaatatcctg
     3661 cgggacgatt cctcgcattc cgcctcgctt aactcggccc ggcgcgcttt catttccgcc
     3721 cggagtaccg tttttgccgc cctgatgtcg ggttccacgt cgttacagct cccactggat
     3781 gctgaaaaac gcgtttctcg gcgccgccgg gatgaagtag ggcgtcgcgt agatggcggt
     3841 ctgcgatgcg accgcgtaca cgtcgtccac gtacccggaa gcctcataca accgatcgaa
     3901 caggttgttg accgacaacc tcacctccat ccccttcagc ccgaagcgtt tttcgatccg
     3961 gtacgcgaga gatacgtcag tgacgtggta cggagcgatg gaattggcag tgttttctgc
     4021 gttgtcgagg tactgccggc ccacgtaccg ccatgtggcg ccgaggctcc acccgccgtg
     4081 cgtcagccgg gcgcgggcgt ttgccaacat ggtggggaac ccggcgatgc ggttgccgtt
     4141 gcgcggcagg gaaacggtct gccaggcatc ccagtccatc tggtgttcgg tgtaccgcac
     4201 gaagtggtcg ttgttgatgc tcacgttccc gccgagttcg aggaaatgcg ccggcgttgc
     4261 ggtcgcttcg agctcgattc cctggtgcac cgaacgatcc gcgttgccgg tgaccgggtt
     4321 gccgctggct tcgcttatct gccccaccca ggggacaatt tcattgcgga agtccatgtg
     4381 gtagagggta agcattgcgt taattcgagg cgaacgatac ccggtgccaa gttcgaaatt
     4441 cagtacgcgt tccggcttga ctgtggggtt cttgagccga atctgcccga tctgcccgcc
     4501 gacgaaggtg gtttcccgct ccgcgaaact cggttcgttc cagtaatcct gcgggtcggc
     4561 gaggtcgtcg gtgatcggtt ccctcctcgc ataggcgatg ttggtgaatg cgtgcaggcg
     4621 atcggtgatg ttgacgttca ccccggcttt cggtgatatc cagcggaact ccgcatcgta
     4681 ccggtaacgg gtgaggctgt cgcgatccag cgtgtacagg tggcgcacgg cctgcaggtc
     4741 ggccatgagg gtgacccgcg gcagaatgtg cgccatttcc tgcacgaacg cagaggtggt
     4801 gagcttccgt tcgcggtagt catagtataa atgaccggga gcaacgcccg gaggcggggc
     4861 cgccagccac gtgatctgtc cccagtggcg cccgctgtgc gcgcgcagtt ctccgcccgc
     4921 cgtcagagtt ccgccgtggt gttcccacgc catgcggggc agcacgccgt attcgcggtt
     4981 ttccacgttt ctgcggcggg tgagatccga cttcgtgatg gtgtagtcgc cattcgcgtc
     5041 cgcgcggggc gttccgtccg cgttccgtgt gtaccacgac agcggataca acgaggggtc
     5101 cttcacgcgg aatgtcggca ggcggaattc ctccagactg cgagccgtgc ggaactgatc
     5161 gtaataccct tccccggtga tcgcgtacag cgtggtgttg aggcgaaggg cgggcgcaat
     5221 ctgccagtcg tggtgcagtt cgtagtgcgg ctggttgaag gtatcgattt cgttctcgta
     5281 ggtaagcggg ttataccggc gatcggcgcg ggcatcgccc gacaccgttc cgtcgagata
     5341 ggcccgcgtt actccattgt aggcgaggtg ggttctttcc ggcccgccgt acacgagcag
     5401 ggtcgtcgtc atggacggat cgaatcgccg gacgccgagg aagtagctcc acaggtcgga
     5461 ccacgattgc tgccggtatc cgtcgctggt aatgcgcgag aaccgcccgt acatcgcgta
     5521 ggagtcgtcc accagcccgc tgttgaatgc cacgctcagt ttgcgggttc cgtaacttcc
     5581 cgcccccgca aaggcggtga cgctgcgctc cgggttgaaa ttggtggtga tgaggttgat
     5641 ggagccgccg attgctgatg agccgtacag cgagctgccg acgccgcgct gcacctggat
     5701 atcctgcacg ttggagagca cgtcggggag atcaacccag tatacctcgt gcgacgtggg
     5761 gccgttctgc ggaatgccgt tgatcagcac gttcactcgc ttctggccga acccgcggac
     5821 gctgagatag ctgtacccta cgccgttgcc cgcgtcggag taggcgtgga cggatgggag
     5881 cgactgcagc aggaccggca cgtcctgcac cgagtaagtg cgggcgatct cctcgcgggg
     5941 gaggtttgtg aacgtgaccg gggcctcgcg gtctttcacg cgggatgcgg tgacgagcac
     6001 cgtctgtccc ggaagcacct cggggtgcag tgcgatggag agcgacaccg gctcatcgcc
     6061 gagggtgacg cgctgtttgt gcaggcggta gccgataagc cccaccgtga cgatatgccg
     6121 cccggcgggc aggccgttga gcgtgaatgc gccgttctgg tcagcgagcg ttccgtgagg
     6181 cgtgttgtcg agatgaatga ccgcggcggt tacgggcgcg cctgtttccg cgtcaatcac
     6241 cacgccggaa agcgtggctg cgtgagcaat gagcggagcg gcgcaccacg atgcgaggaa
     6301 acaaaaaacg agcgagcgca tgatacctcc ttgtttgcgc aacgcccggg catgctgcac
     6361 acgcaaaaag ccgcccccgg ggtctggttc gggaacggct ggtaactcca cagaacttgt
     6421 gtgggcgttt ccctacgctg gcattaccca ggtcaggttc atggggtata gtctcaggcc
     6481 ttccacggga gggccacccc caacgacacc attgtagtag gaaatatacc cgcacgatgg
     6541 gcgacgggca agaactgcgt gatttattcg cagcgcggag gttctggatt cccgccttcg
     6601 cgggaatgac tacgccgggc ggatggcgga gtgggctctg ccgacagcga tgagtttcta
     6661 agagggcgcg ccgtcggcgg aggtttcaca ctgaacagag cacgtttgga gtacgctccc
     6721 gcgggtatgc tcttcgcagt atccgccatt ccgcgtgctc ggccgagcac gaagcgcagg
     6781 cgggaatcca gtggcgcgag ggcccgcaaa tcgaccccac gacaccggca ggacgcgaac
     6841 tctacggtcg tcgtacgtcg gaacgaacgg cggacgagtg gcgaaccggt cgctgagtgt
     6901 gtcgaagcgc cggttcatcg cggctatacg accattagcg ctccatcgcg aggtgtgtat
     6961 gggtcgggtt gcccgacccc cttatctttt ctggcaagag aacgaagcgc gagtgaagga
     7021 aagggggtga gccacgatgc gacgccaatt gattgccccc acccggatac ttgtagccct
     7081 gttacttccc gcgctctgcg ttgccgccga acagagcgag ggagaggcgc gcgaagctcc
     7141 gtccccgccg atgtcggtga tggtgagcgt ggagcgaacc ggcgctgcgg ccatccttgg
     7201 caccaattcc gccgggtcgt ccaccgagtt cgccattacg gcgaagtggc tcgttctcgt
     7261 attcgaagct gattaccggg acatgggctg gagccggcgt tcgttggtgg cgcccgcggg
     7321 tttgtccggc agcacggagt ggggctctct cacacgtctg gctccgggca ttcagtacta
     7381 ccagagcgtc ggcaggcggt gggcgatctg gggcaagttc tccgccattg cggggttcga
     7441 ggaaaccccc tcgttgcgct ccgtgacgta gaatccgcag ttcgtggctc tccggacact
     7501 gaaggacggc acggtcctct attgcggcgc gggttccctc taccaccgcg cgagcaaaac
     7561 ctttttccga tcgtcggcgt cgccaggaac accgatgcga ggcaggggtt ttccgccgtg
     7621 atcgcattcc ccgaggcgta cgcgcggtac cggttcaacg agcgtgttgc gctgcgggcg
     7681 gactacgagt gggacacgcg tgtgttcggg cttgcggcca acgacccgac cgtacaggac
     7741 ggttacctca ggatccgcga ccattacccc gctctcaacg ttgagtacac cccggca
//
LOCUS       DAOTNG010000139         7790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8288_length_7790_cov_5.431286, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000139 DAOTNG010000000
VERSION     DAOTNG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_7790_cov_5.431286"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="P5179_07910"
     CDS             <1..963
                     /locus_tag="P5179_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95180.1"
                     /translation="VDWLSPQVWRRDSDVPCVSLGPPGAFDDAHIFAPCVAFEDGLFR
                     MWYCGSRGTVEGRVFRMGFATSFDGVNFTKHPASPVLSFGDTRSILTPTLLRHPGGSL
                     CREDGCLRMWFSSTDFPAGDGVHTLHETTGVDGLTWSPPSRPLLHNLYAPTIIKQDSV
                     YRMWYTDVVADPWCFRYAESGDGTRWDVVPQPVMVLDQAWEHGRLFYPTVLVADGLHL
                     MWYGSYSHRKGEETRTALGFAVSKDGITWEKSPSNPVFGPDPAHAWESNFTTSQSVLW
                     LPDRSLRIWYASRPKPPFTHKYFAIGTARCIPGTAGRMRRPEAG"
     gene            1019..1366
                     /locus_tag="P5179_07915"
     CDS             1019..1366
                     /locus_tag="P5179_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95181.1"
                     /translation="MLELKHEPVLPIRQFVGRLLGFTAAAVLLVVIALGIGVCGYYWI
                     AGLGFVDALLNASMILGGMGPVDPLRGDAAKLFASAYALFSGLVFLTVAAMLFTPLVH
                     RMLHRFHVDETGQ"
     gene            complement(1398..1937)
                     /gene="hslV"
                     /locus_tag="P5179_07920"
     CDS             complement(1398..1937)
                     /gene="hslV"
                     /locus_tag="P5179_07920"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041467627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRS95182.1"
                     /translation="MNYPEIYSTTVLGIVRDGVAVIGGDGQVTLGNTVVKRSTVKVRK
                     LANNTVLAGFAGGAADAFALLEHFEGKLEETRGNLPRAALSLARDWRTDKFLRRLEAF
                     LIVMDKQQGFILSGTGDVIQPEDGVMAIGSGASYALAAAKAYLDGSGLPVREIVERSL
                     RIASDICIYTNATLTIEEL"
     gene            complement(2150..3055)
                     /locus_tag="P5179_07925"
     CDS             complement(2150..3055)
                     /locus_tag="P5179_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRS95183.1"
                     /translation="MRAAIGDFIQYLIGERNYSQHTARAYRADLLQFAEYLEEELAHP
                     PALRDLTRGYVRTYVTWLMETGHKQRTIARKLASIRSFQTYLQRHDLVSEQDWGRLAP
                     RQQQRTLPTVLDADQLALLMTLPPNEDPTGVRDRAIMELLYSSGLRVSELVALDLEHL
                     DIAEGLVRVRGKGNKERIVPVGTIALEALDRYIREFRPLLIPPALRRRKFSPLFVSKL
                     GRRLSVERVRAMVTGYLRQVSNSEHLGPHLLRHACATHLVERGAGLRDVQEMLGHSSV
                     TSTQIYVSVASRRLTQIYGKSHPRA"
     gene            complement(3089..5596)
                     /gene="topA"
                     /locus_tag="P5179_07930"
     CDS             complement(3089..5596)
                     /gene="topA"
                     /locus_tag="P5179_07930"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRS95184.1"
                     /translation="MPRSLVIVESPAKARTINKVLGREFDVRSSMGHVRDLPENSLGV
                     NVEEDFLPQYEVLPSKRKIVNELRMAGKNAERVYLATDPDREGEAIAWHIAEVMRLGK
                     NRTRRITFHEITPRAIKEALDHPTELDMHKVDAQQARRVLDRLVGYQVSPLLWQTVRK
                     GLSAGRVQSVALRMVCEREEEVLAFKPREYWTVLVALKNGAVAFEADLWKVDGKLAKI
                     PDEAHAKALVEELTRGQFDVVSLERKRQKRRAFPPFITSTLQQEAARKLGFSTRMTMS
                     VAQELYEGVEVKGELTGLITYMRTDSTHLAPEAVSAARQYIAETYGKEYVPARPNVFR
                     NSKSAQEAHEAIRPTSMDRPPEAVQGELSRDQFRLYQLIWNRFVSSQMEAQELDVTEL
                     VAAGGRFQLRTTASVVAFDGFTRVYLEGQDEEKEEQEHPIPRELIAAWDRTEQGAKRS
                     AEPLLNGYAPAKVLPEQHFTKPPSRFTEATLVKELESRGIGRPSTYAQIISTILDREY
                     VARKEGKLVPTELGKIVNRILSSQFPEIFNSEFTAKMEEELDRVEQGSDHWKSVLKEF
                     YSGFEATLSSAMKRRSEIKKLTVEQSDRVCEKCGKPMVVRLGPRGRFLACSGFPECRN
                     TVALDTEAAPPEIPVPDGVTCPKCGSGMTVRNGRYGVYLACEKYPSCRGARPIPTGVP
                     CPRPGCNGELVIRTSKRGKHYYACDQAGCTVLYWDKPVPLPEPDPATGLRFQLEKTRR
                     DGTTIVSPGVYPPPALRRRSTAAENRSGARTGSRYSRSARAGTKAAEAEPTSGRRGTT
                     SRKSSRSSSAAVGATPSAAGKRSSAKRSAGGTGNARK"
     gene            complement(5798..6748)
                     /locus_tag="P5179_07935"
     CDS             complement(5798..6748)
                     /locus_tag="P5179_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS95185.1"
                     /translation="MPDQAPRHVADIVPGETVTMFLALRGKQLREHEGKPFLRFEFAD
                     RTGRIMAVTSDNMDGFWHAADQNDVVKVRGVVGTWNGKKTLRIQQMRRAKPGEFDAGD
                     FLPRYEGDTDALWKELVAYAESVTDPPLRSVLGNLVDSEEMRGRFLQAPAGKLWHHTY
                     LGGLAEHTANVVALVDSACKRYPLADRNLAIAGGLLHDIGKIEAFDVSTTIEYGDAGR
                     LVSHIVLGERIVAEWCRCVPELPAGHADKLCHIVLAHELSGDQRSPISPMTLEAAIVA
                     AANQLDATAGAFARILTRERDTGQAWSAWVNTLERHVFLK"
     gene            complement(6757..>7790)
                     /gene="hisC"
                     /locus_tag="P5179_07940"
     CDS             complement(6757..>7790)
                     /gene="hisC"
                     /locus_tag="P5179_07940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRS95186.1"
                     /translation="LPAYTLAALETKIKLNQNENPYEIPADLKEEVLRFALERPWGRY
                     PDFVPDEFLALLAAHVGWVREGMLAGNGSNELIQAILAVTCGPGTKVLIVQPTFTLYK
                     LLSRINGAEVVEVLLRREDLAYDIPAVCAAIDENEPAVVILCSPNNPTGSALTLGEWR
                     QVCERAPGLVVADQAYVEFGGDCVAPLLKEFPNLVMLRTFSKAGTLAGLRVGYCAASP
                     DIAEQIAKAKLPYNLNFFSMAVAAMVVRNWHRFEPVIARIRSERERVYAALGSMTGVR
                     VYPSAANFLLFETDRPPAEVFRGVYDRGALIRDVSKYPLLGKALRVSIGTPEENDAFL
                     SALRATLGA"
BASE COUNT         1356 a         2529 c         2337 g         1568 t
ORIGIN      
        1 gtggattggt tgagcccgca agtctggcgg cgtgattctg acgttccctg cgtgtcactg
       61 gggccgccgg gcgcatttga cgacgcgcac atcttcgcgc cgtgcgtcgc gttcgaggat
      121 ggcctcttcc gcatgtggta ctgcggttca cgcggcaccg ttgagggacg cgtgttcaga
      181 atggggtttg ccacctcctt cgacggtgtc aacttcacga agcaccccgc gtcgcctgtg
      241 ctgtcattcg gcgacacgcg gtccatcctc acgccgaccc ttctgcggca cccgggcggc
      301 tccctgtgcc gcgaagatgg ctgcctgcgc atgtggtttt caagcaccga ttttcccgcc
      361 ggcgacggcg tgcacaccct gcacgaaacc accggcgtcg acggcctgac ctggtcgccg
      421 ccttccaggc cgctgctcca caacctgtat gcccccacga tcatcaaaca ggactccgtc
      481 taccgcatgt ggtataccga cgtcgtggct gacccgtggt gctttcgcta cgccgaaagc
      541 ggggacggca cccggtggga cgtggtgccg caaccggtca tggtgctgga ccaggcgtgg
      601 gagcacgggc ggctcttcta cccgaccgtg ctggttgccg acggcctcca tctgatgtgg
      661 tacggaagct attcgcatcg caagggagag gagacgcgca cggcgctcgg gtttgcggtg
      721 agtaaggatg gtattacgtg ggaaaagagc ccgtcaaacc cggtatttgg ccccgatccg
      781 gcccacgcgt gggagtccaa cttcaccacc agtcaatcgg tgctgtggct gccggaccgg
      841 tcactgcgga tctggtatgc atcccgcccg aagccaccat tcacgcacaa gtatttcgcg
      901 atcggcacgg cgcggtgtat accggggacg gccgggcgga tgcggcggcc cgaggccggg
      961 tgaggcgaga ctgacgcgaa ccccgtccgg accgatttgc ttttttgtga ggtgaaccat
     1021 gctggaattg aaacatgaac cggtactgcc gattcggcaa ttcgtcggcc gattgcttgg
     1081 tttcacggcg gcggcggtgc tgctggtcgt gatcgcgctg ggaatagggg tgtgcggata
     1141 ctactggatc gccggtctgg ggtttgtcga tgcgctcctg aacgcgtcca tgattctcgg
     1201 tggtatgggg ccggtggatc cccttcgcgg cgacgcggcc aaactcttcg cgtctgcgta
     1261 tgccctgttc agcggcctcg tgttcctcac ggtcgctgcc atgctgttta cgccgctggt
     1321 gcaccggatg ctccaccggt tccacgtgga cgagaccggt cagtgacgtg cgttgtgcgc
     1381 ggcgtggcgt tgccgcctca cagctcctca atcgtcagtg tggcgttggt gtagatgcag
     1441 atgtccgatg cgatgcggag gctgcgttcc acgatttccc gcacaggcag accagacccg
     1501 tcgagatacg ccttcgcggc cgccagcgcg tacgaggcgc ccgaaccgat cgccatcact
     1561 ccgtcctccg gctggatgac gtccccggtg ccggagagaa tgaatccctg ctgtttgtcc
     1621 attacgatga ggaacgcctc gagccggcgc aggaacttat cggtgcgcca gtcgcgggcg
     1681 agagacagcg ccgcccgcgg aagattgccc cgggtttctt ccagcttgcc ctcgaagtgc
     1741 tccagcaggg cgaacgcatc cgcagcgcca ccggcgaagc ccgccagaac ggtgttgttg
     1801 gccagtttgc gcaccttcac ggtgctccgc ttcaccaccg tattgcccag cgtcacctgt
     1861 ccgtcaccgc caataacggc aaccccgtcc cgcacgatgc cgaggacggt ggtggaatag
     1921 atttcggggt agttcatgtg tcacctttct gactttcctg atggtatcgc gcatcactct
     1981 gcacgcgggg cttcgacagg ctgcccgggg ggcaggtcgc tcagccaccg cgtgcaatag
     2041 tcagcggcgt ttctctcgcg cggtcgttga gtggcgcggc atctcgccgc gctgtatcga
     2101 aacgccgcgc aaaaaccgta gccaccatct tatccccacg cggggcgatt taagccctcg
     2161 gatgcgactt cccgtaaatc tgcgtcaacc gccggctcgc cacggatacg taaatctgcg
     2221 tagacgtcac actcgagtgt cccagcatct cctgaacatc ccgcaacccg gcaccacgct
     2281 ccacaagatg agtcgcgcag gcgtgacgca acagatgagg tcccagatgt tccgagttac
     2341 tcacctgccg cagatatcct gtcaccatcg cgcggacgcg ctccacactg agccgcctgc
     2401 cgagtttgct cacgaagagc ggcgaaaact tgcggcggcg caatgcggga ggaatgagca
     2461 gcgggcggaa ctcgcggatg tatcggtcca gtgcctccag cgcgatcgtt ccgacgggga
     2521 ctatgcgctc cttgttccct ttcccgcgca cccgcaccag tccctcggca atatcgaggt
     2581 gttcgaggtc gagcgcgacg agttccgaca cgcgcagccc ggaggagtac aacagctcca
     2641 tgattgcccg gtcgcgcact cctgtcgggt cttcattcgg gggcaacgtc ataaggagcg
     2701 ccagttggtc ggcgtccagc accgtcggaa gcgtgcgctg ctgctggcgg ggcgcaagcc
     2761 gcccccagtc ctgctcgctg acgagatcat gccgctgaag atatgtctga aacgatcgga
     2821 tactggcgag tttgcgggcg atcgtgcgct gtttgtgccc ggtctccatc aaccaggtga
     2881 cgtaggtccg cacgtatcct cgggtaaggt cgcgcagcgc cggcgggtgt gccagttcct
     2941 cttcgaggta ctcggcgaac tgcagaaggt cggcgcggta cgcacgggcg gtgtgctgcg
     3001 agtagttccg ctcgccgatg aggtactgaa tgaaatctcc gatagccgct cgcatcagat
     3061 atttcccccc ggcgccgcgg cggatcgttt acttccgcgc gttgccggtc ccgcccgccg
     3121 accgtttcgc ggacgacctt tttcccgccg cagatggtgt cgcgccaacg gcagcgctgc
     3181 tgcttcgact cgatttcctc gatgtcgttc cacgccgacc ggacgtcggt tcggcctcag
     3241 ccgccttcgt gccggcgcgg gctgatcgcg aatacctgct ccctgttcgc gcacccgacc
     3301 gattttcggc ggcggtgctt cgtcgccgca gtgccggagg gggatacaca ccgggagaca
     3361 cgatggtcgt gccatcgcgc cgcgttttct caagctggaa ccggaggccg gttgccggat
     3421 cgggttccgg aagtggaacc ggtttatccc agtacaggac ggtgcagccc gcctggtcgc
     3481 aggcgtagta gtgcttccct cgcttgctag tccgtatgac aagttccccg ttgcaaccgg
     3541 gccgcgggca cggtaccccg gtcggaatag gacgcgcgcc acggcacgag ggatacttct
     3601 cgcacgcaag atacaccccg taacgcccgt tgcgcaccgt catcccgctc ccgcatttcg
     3661 ggcatgtgac gccgtccggc accggaattt ccggcggcgc ggcttccgtg tccagtgcaa
     3721 ccgtgttccg acactccgga aagcccgaac acgccagaaa acgccccctc ggccccagcc
     3781 ggacgaccat cggcttgccg cacttctcgc acacgcggtc agattgttcc accgtgagct
     3841 tcttgatctc cgatcggcgt ttcattgcgc tggaaagcgt cgcctcaaag ccgctgtaga
     3901 actccttcag caccgatttc cagtgatcgc tgccctgctc gacgcggtcc agctcctctt
     3961 ccatcttcgc cgtgaactcg ctgttgaaga tttctggaaa ctgcgaggac agaatacgat
     4021 tcacgatttt gcccagttct gtcggaacca gcttcccctc tttccgcgcc acatactcgc
     4081 gatcgagaat cgtgctgatg atctgagcat acgtgctggg acggccaatt ccccgtgatt
     4141 ccagctcttt gaccagcgtt gcctcggtga accgtgaagg cggcttggtg aaatgctgct
     4201 cagggagcac ctttgccggt gcatacccgt tcagaagtgg ctcggcggaa cgtttggcgc
     4261 cctgttccgt gcggtcccac gccgcgataa gttcacgcgg tatcggatgt tcctgttctt
     4321 ctttctcttc gtcctgtccc tcaaggtaga cgcgcgtgaa cccgtcaaac gcgacaacac
     4381 tcgcggtcgt gcgaagctgg aatcgtccgc ccgccgcaac aagttccgtc acgtcgagtt
     4441 cctgcgcctc catctgtgag gagacaaaac ggttccagat cagctggtac aggcggaact
     4501 gatcgcggga aagctcgccc tgaacagctt ccggcggccg atccatcgaa gtcggccgga
     4561 tggcctcatg tgcctcctgt gcagatttac tgttccggaa cacattcggc cgcgccggaa
     4621 cgtactcttt gccgtacgtc tccgcgatgt actgccgcgc agccgaaacc gcctccggcg
     4681 ccaaatgcgt cgaatcggtc cgcatatagg tgataagacc ggtgagttcg ccctttacct
     4741 caacgccttc gtacaactcc tgcgcaacgc tcatggtcat tctggtggag aacccgagtt
     4801 tgcgggctgc ctcctgctgg agcgtgcttg taatgaacgg cgggaacgcg cggcgtttct
     4861 ggcgcttgcg ttcgaggctc actacatcaa actgaccgcg ggtaagctcc tccaccagcg
     4921 ctttggcgtg tgcctcgtcg ggtattttcg cgagtttgcc gtcaaccttc cagaggtcgg
     4981 cctcgaacgc aacggctccg ttcttgagcg cgacaagtac cgtccagtat tccctcggct
     5041 tgaacgcgag aacctcctcc tcgcgctcgc ataccatgcg caacgccacc gattgaacgc
     5101 gccccgcgct caatcccttg cgcacggtct gccagaggag agggctcacc tggtatccga
     5161 ccaggcgatc cagcacgcgg cgcgcctgtt gggcgtccac cttgtgcata tccagctcgg
     5221 tgggatggtc cagcgcctcc ttgattgccc gcggcgtaat ctcgtggaag gtgatccttc
     5281 gcgtgcggtt tttccccagt cgcatgactt cagcaatgtg ccaggcgatc gcctctccct
     5341 cgcggtcggg gtccgtcgca aggtataccc gttccgcgtt ctttccggcc atgcgcagct
     5401 cgttcactat tttccgcttg ctgggaagca cttcgtattg cgggaggaaa tcctcctcca
     5461 cgttcacccc gagggagttc tcaggaagat cccggacatg gcccatggag gaacgaacgt
     5521 cgaattcgcg tccaaggact ttgttgatgg tgcgggcttt tgcgggtgat tcaacaatga
     5581 caagcgaacg tggcatggtt actccagaat cgcggttcgg gcgatcggcg ttgagattac
     5641 tatcagcgcg gaggataagg ggttacaatc tttctgataa tagacggcga acaggaggac
     5701 ggcaagtctc ggctgtgaaa gcgactcttc tgcgtctcgt ctgatgtcgg cgatctggcg
     5761 ttccgcggaa catgcgggaa ccgtcgccac ggcatggtca tttcagaaat acgtgccgct
     5821 cgagtgtatt cacccaggcc gaccaggctt gcccggtatc gcgttctctc gtgagaatcc
     5881 gggcgaacgc gccggccgtg gcgtcaagct ggttcgccgc ggcaacgatc gcggcctcca
     5941 gcgtcatggg cgatatggga gagcgttgat cgccggaaag ctcgtgtgcc aacacgatat
     6001 ggcacagctt gtcggcgtgg cccgcgggaa gttcgggaac gcaacggcac cactccgcga
     6061 cgatgcgctc tcccaagaca atatggctca caaggcgccc ggcatccccg tattcgattg
     6121 tggtggagac gtcgaacgct tcaatcttcc cgatatcatg aagaagcccg ccggcgatgg
     6181 cgaggttcct gtcagcaaga ggatatcgtt tgcacgccga atccacaagg gcaaccacat
     6241 tcgccgtatg ttccgccaag cctccgaggt acgtgtggtg ccagagtttt cccgcgggcg
     6301 cctgcaggaa ccggccacgc atctcttccg aatctaccag attacccagc acggagcgaa
     6361 gtggcgggtc ggtaaccgat tccgcatacg cgacaagctc cttccagagc gcgtccgtat
     6421 ccccttcgta gcggggaagg aagtcccccg catcgaattc ccccggcttt gcgcgccgca
     6481 tctgctggat gcgcagcgtt ttcttaccgt tccacgtgcc gacaaccccg cgcactttca
     6541 ccacgtcgtt ctggtctgcg gcgtgccaga agccgtccat gttgtcgctt gtcaccgcca
     6601 tgatccgccc ggtacggtcg gcgaactcga aacgaagaaa cggcttcccc tcgtgctccc
     6661 tcaattgttt cccgcgcagg gcgaggaaca tcgtcacggt ttcacccgga acgatgtcgg
     6721 caacgtgacg cggggcctgg tcgggcatgg cgtgcctcac gcacccaatg tcgcgcgaag
     6781 tgcggaaagg aaggcatcgt tttcctccgg cgtgccgatg ctcaccctca gcgcctttcc
     6841 gagaagcggg tacttgctta cgtcgcggat caatgcgccc cggtcgtata caccacggaa
     6901 cacttccgca ggcggtcggt cggtttcgaa caggaggaag ttggcggcgg aaggatacac
     6961 gcgcacaccc gtcatgctcc cgagcgccgc gtacacccgt tcccgctccg aacggatccg
     7021 tgcgatgacc ggttcgaacc ggtgccagtt ccgcaccacc atggcggcaa cggccatcga
     7081 gaaaaagttc aggttgtacg gcaatttcgc cttggcgatc tgttccgcga tgtcgggcga
     7141 cgcggcgcaa taccccacgc gcaggcccgc cagcgttccc gccttggaga acgtgcgcag
     7201 cattacaagg ttcgggaact ccttcagaag gggcgctacg cagtccccgc cgaactccac
     7261 atacgcctga tccgccacca ccagcccggg cgcccgctcg catacctgcc gccattcgcc
     7321 cagcgtaagc gcgctgccgg tagggttatt tggcgagcag agaatcacta ccgcgggttc
     7381 attttcgtca attgcggcgc acaccgccgg gatgtcgtac gccagatcct cgcggcgtag
     7441 caacacctcc accacctccg cgccgttgat gcggctcagg agcttgtaca aggtgaacgt
     7501 cggctgaaca atcagcacct tcgtgccggg cccgcacgta accgcgagga tagcctgaat
     7561 cagctcgttc gagccgttcc cggccagcat cccctcgcgc acccagccga cgtgcgctgc
     7621 cagaagggcg aggaactcat ccggcacgaa atccggatac cgcccccacg gccgctccag
     7681 cgcgaagcgc agcacctcct ccttgaggtc cgccgggatt tcgtacgggt tttcgttctg
     7741 gttcagcttg atcttcgtct caagcgccgc cagcgtgtac gccggcagcg
//
LOCUS       DAOTNG010000140         7629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8478_length_7629_cov_5.667019, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000140 DAOTNG010000000
VERSION     DAOTNG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_7629_cov_5.667019"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="P5179_07945"
     CDS             complement(<1..697)
                     /locus_tag="P5179_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRS95187.1"
                     /translation="MALKTGRAEGSATVTIDRALCDACGCCVRVCSADVLRMENGAVV
                     ADTTRLFGCVGCSHCMLVCPTDAIAVHGRDTSPADVCELPPPEMRADFDSLLALLLSR
                     RSVRRFRSSEVGEEAIQKIVEAVSTAPMGLPPSDVEILVLRGRPRVQEFAADIVEAIR
                     RSQWFVSPLMTTLMRPFIGKEAAEMFRTFLRPLAHALPEERARGEDWLLYDAPLAMYF
                     HTSPYSDPADPVIA"
     gene            888..2168
                     /locus_tag="P5179_07950"
     CDS             888..2168
                     /locus_tag="P5179_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95188.1"
                     /translation="MSELYQRIAAAVDAIPLFDTHEHFYDEATRLNAKKEVDLFYFLH
                     YVECDIKSAGSPDEAGEFAGGTLSTDEKRRRFLKYWPYVQDTGYGRAFRIMANDLWDV
                     PEINEGTYDLLADRATNFARPGFYKTVLRDRANVARSIRIVWAGQNTLCDLDYLFPAP
                     VFDNFVGPTSRADIRKLEAQTDTRIHSLRDLETALDAAFDRRQNEGMVAVKIFLAYRR
                     NLDFAHTTTAAAEAALQRVLVALPPSPLGVDETKPLQDYMVHQIVQRAVERKLPVKIH
                     TGYQNSNDNVFANGKPEPLINLVMSYPTARFVFLHCGWPYTDQVLAFAKMFPNVFPDM
                     AWAHILAPGMAQRLLHDLLDTVPSNKILGFGGDYNFAEGAYAHAKLARAGISKVLAKR
                     VEEGVFTEEQALVIAKRIMHDNAAALYGFPSLDA"
     gene            complement(2240..4222)
                     /locus_tag="P5179_07955"
     CDS             complement(2240..4222)
                     /locus_tag="P5179_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS95189.1"
                     /translation="MRKRETKRIARFAALMALAILSMSQAVGAARSTFVQEIIITSTT
                     VPAPVGDEKWRQVPERRKLNGILQHPTPSFRNLWLPVRFDDVLFAADTMSVLTYYRNL
                     GYLGAYVATTAQEPVGGRLEGLQYVNLLIVIGGITPEERYALHSIEFSGVTSLDTAAL
                     AKEFRKRHNAGKFFSPSGAAENFVALRTAYADAGYLDSNAVTIRQLPIVDTLARSVRE
                     RYEVEERTPTTVTGYAIINGNAPLPLITDSAVVVQALKDAKLVPGSVFGRQRMLDAES
                     NLFDLGVFRRARVAPDTAFARGSPFQRRAMVDIAERDAGDVRVQLGYSNIQQWRISET
                     ATYSNFLGEARLIGQEGQLSRDVQALSFMYGQPRIGVPSFVPLVGETSMRLRLDQALS
                     GSWEVLAAGDTSRTFAWKTSLSRRFGRMARIGISFDLSRSDTTSRFLFSRPRSGATLQ
                     SAVSVNALYDTRDEFLNPSRGFALNAQVRVVNPSHRVSRMSMRPEALAVYYRPVSPFV
                     IGAVSLGGGFYYVGKDDSLNQAEQFWRSRQMPTVRGFVRDDITVIRGDSVGSPAVAYL
                     LLKTEARIALWKRLSSAVFVDAGQAWVWIPRAVRNPTAEERAFRGWSAMEAGRFALSA
                     GFGPRLNWGLMFRLDLVRQITPSTHWTYEFGITQAF"
     gene            complement(4269..>7629)
                     /locus_tag="P5179_07960"
     CDS             complement(4269..>7629)
                     /locus_tag="P5179_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRS95190.1"
                     /translation="TVSATLTSVDSVVRADAVIVNKFMRFASLRASIGPLPFEQLPGG
                     FAKEQDITGSLSADIVGAGLLTNPYLRGSIRTTPVAWHGQQIGTIRGDLNWFGNSLTL
                     DVRNGNDTFSAQGRVAADSGATSQIDVQWENFDLGGILSALTDQPAGKISGMTDGTIS
                     ARWTRLVVNSLVIQARLDRASLRLGQATYELADPPSRFSMEKGVLDLTPTLFRGSNDQ
                     RLHLQGSISRSGEMSFRAALDSFDVGNAISTLTDGKLAISGKMVLRAHLGGTLEEPVA
                     NGRVTFRGIRWKTVEIDSAGALATYSSNQLSVPEFSFRFPFGEARGSFRATASALGLA
                     DTATGPPDFALSLVMKDAGAVVPNWEGLRSGTVSISGELALRGRDLADISSYRGSFRL
                     DSLAAVAPFNRLVRTKRPATVAIGESRYLASPLDLAMSYRGAETGEISISQPPGVARP
                     PLDVSINRVSLEDFRHTILPLLNALDAQTSVPEDLSGEFSGHARWDMNLSSPRVDMSF
                     LIREPTAYSLRGDSLDCVAAVRDSGVWVESANFYTAGDTLNVTGFVDLSADTLLFHAY
                     TANLELVPVLTDLLPPLRTYQPPPPLPTTAPFPGFPQQIRALPAEARGATVNAPLTTF
                     DRLVRARRGAMKHPYAATLPMAAEFELRGTLEFPLLFGNAQLFGAYLEIEALQEPIWL
                     PDTLSIRVTGKRAVVPPVRVFVGAQQRETQTPPAISLERAEYSLSRGEFILEAKIERA
                     TFSLVSTEPVFLPPRWRLFKGVLEPLLRAYYTEPIGVFTASATGSWRGTMEHSNLNAD
                     VVLYSSTLQYPIADPQAIFKPRAPTSLGPLLDNTELNISLSMEDSLVINNNLSKHATL
                     WAQMNLTGRHYSPNLQGNVQISPGSVFRYLGRDFVIQEATVNFPDPTRFAPEIEVTAR
                     AKVVNTKDNNAPSYDVTLHASGVVPNLVKTEMTAEELPSGEVIVNQLEIMQILIYGQR
                     SPDYLAIDAQGRLNTMLKERGYATLSSALGSVLPIDQVTVRESADNGTQQGTGGMNDV
                     EVEVGQNVRVWGQKLTFSAVSPVGQLSSVSGLERAEVRWTILERPPRWPNLESLSLTV
                     GQNRLQQAQASTDLYLQNELTSGLQLRVRFR"
BASE COUNT         1535 a         2332 c         2261 g         1501 t
ORIGIN      
        1 cggcaatgac agggtctgcg gggtctgagt acggcgaggt gtggaagtac atggcaagag
       61 gcgcgtcgta cagaagccaa tcctcacctc gagcgcgttc ttcggggagc gcgtgcgcaa
      121 ggggccgcag aaaagtgcgg aacatttcgg ctgcctcctt gccgatgaac ggccgcatga
      181 gcgtggtcat cagcggcgaa acgaaccatt gggagcgcct gatcgcttct acaatgtcgg
      241 ctgcgaactc ctggacccgc ggccttcccc tgagcacgag gatctcaacg tccgatggcg
      301 gcagtcccat cggggcagtt gacacggctt caacgatctt ctggatcgcc tcctccccca
      361 cttccgacga acgaaatctc cgaacgcttc tccgtgacaa gagaagagcc agcagtgaat
      421 cgaaatccgc gcgcatttcc ggtggtggaa gttcgcacac gtccgccggt gacgtatccc
      481 gtccgtgaac ggcaatcgcg tcggtaggac agaccagcat gcaatggctg cacccgacgc
      541 acccgaaaag acgggtggtg tcggcgacga cggccccgtt ctccatgcgg agaacatcgg
      601 cagagcacac gcggacacag cacccgcacg catcacacag ggcgcgatca atcgtgactg
      661 ttgccgatcc ttcggccctt cccgtcttca atgccatctg gcacctcgtg gttcgagtgg
      721 aggtaactac gcgaacgcga acttgccccg ccaaccgaat atggcatggt ccggcgtccg
      781 ggggagaggt tccccgtccg gcactgcttg ggaactcgtc tccccgtctg gtacattcct
      841 tttcgtaacc gatccgactc aaggtccgcc cacaggagca ggaaccgatg tccgaacttt
      901 accaacgaat tgccgccgcg gttgacgcaa tccccctgtt cgacacgcac gagcatttct
      961 acgacgaagc aacgcgattg aatgccaaaa aggaggtaga tcttttctac ttcctccact
     1021 atgtggaatg cgatatcaaa tcggcaggct cgccggacga agcgggcgaa ttcgccggag
     1081 gcacgttatc taccgacgag aagcgcaggc ggtttttgaa atactggcca tatgtgcagg
     1141 ataccgggta cggacgggca ttcagaatca tggcgaacga cctgtgggat gtgcctgaga
     1201 tcaacgaagg aacctacgac ctgctcgcgg accgcgcaac caactttgca agacccggtt
     1261 tctacaaaac cgtgctcagg gaccgtgcga acgttgcccg ctcaattcgc attgtctggg
     1321 cagggcagaa caccctttgt gatctggatt atctcttccc cgcgccggtg ttcgacaact
     1381 ttgtcggacc gaccagccgc gccgatatcc gcaaactcga agcgcagacc gacacacgca
     1441 ttcactcgct ccgcgacctt gagactgcac tggacgcagc gtttgacaga cgccagaacg
     1501 aagggatggt cgctgtcaag atcttccttg cttaccgccg gaatcttgat tttgctcaca
     1561 cgaccactgc cgcggcagaa gcggctcttc aacgggtgct cgtggccctt ccgccatctc
     1621 cactcggggt ggacgaaacg aagcccctgc aggattacat ggtgcaccag atcgtccagc
     1681 gcgcggtcga acggaagctg cccgtcaaga ttcacaccgg gtatcagaac agcaacgaca
     1741 atgtgttcgc caacggaaaa ccggaaccac tcatcaatct ggtcatgtcc tacccgaccg
     1801 cccgtttcgt gtttctccac tgcgggtggc cttacaccga tcaggttctt gcgttcgcga
     1861 agatgtttcc caacgtgttc cccgacatgg cgtgggcgca cattcttgcc cccggcatgg
     1921 cacagcggct tcttcacgac ttgctggata ccgtcccttc gaacaaaatc ctggggtttg
     1981 ggggcgacta caacttcgcg gaaggtgcgt atgcccacgc gaagctggct cgagcaggta
     2041 tttcgaaagt ccttgcgaaa cgcgtggaag agggagtgtt caccgaggag caggcactcg
     2101 tgatcgcgaa gcgcatcatg catgacaatg cggcggcgct gtacgggttc ccttctctgg
     2161 atgcctgatg gtcaaaggga tgtgtggtac gccccggggg agctgaacgg ctcccctttt
     2221 tggtgtagat caggcgaaat cagaaggcct gtgtgatgcc gaattcgtag gtccagtgag
     2281 tggacggcgt gatctgccgg accagatcga gccggaacat gagcccccag ttcagccttg
     2341 gtccgaatcc ggcgctgagc gcgaaacgtc cggcttccat ggcgctccac ccccgaaacg
     2401 cacgttcctc ggcggtagga ttgcgcaccg cgcggggaat ccatacccag gcctgccccg
     2461 catctacaaa cacggccgaa ctaagacgct tccagagcgc gatgcgcgcc tccgttttga
     2521 ggagcagata cgcgaccgcg gggctgccga cggaatcgcc gcggatgacc gtgatatcgt
     2581 cacgaacgaa ccctcgtacg gtgggcattt gcctgctcct ccagaactgc tcggcctggt
     2641 tcaggctgtc gtcttttccc acgtagtaga agcccccgcc cagagacacc gcgccgatta
     2701 cgaacggcga caccgggcga tagtatacgg ccagcgcctc gggacgcata ctcattcgcg
     2761 aaacgcggtg gctcgggttc acaactctca cctgggcgtt gagcgcaaac ccgcgcgaag
     2821 ggttgagaaa ctcgtcgcgt gtgtcgtaga gggcgtttac actcaccgcg ctttgcaacg
     2881 tggcaccact tcggggcctg ctgaaaagga accgggaggt agtgtcgctt cgcgagaggt
     2941 cgaacgaaat cccgattcgc gccatccgcc caaagcgacg gctgagagag gtcttccacg
     3001 cgaacgtccg tgacgtgtct cccgcggcaa gcacctccca gcttccggac aacgcctggt
     3061 cgagccgcaa ccgcatgctc gtttcgccca ccagcggcac gaaggaaggc accccgatac
     3121 gcggctgccc gtacatgaaa gacagcgcct gaacgtcccg cgacagttgc ccttcctggc
     3181 cgatcaggcg cgcttctcca aggaagttgc tgtacgttgc ggtctcgctg atccgccact
     3241 gctggatgtt cgagtatccc agttgcaccc ggacgtctcc cgcgtcgcgt tcggcgatgt
     3301 ctaccatcgc gcgccgctgg aatggtgatc cgcgggcgaa cgcggtgtcg ggcgcaacgc
     3361 gggcgcgtcg gaacacgcca aggtcaaaca ggttgctttc cgcgtcgagc atccgttgcc
     3421 ggccgaagac ggatccgggg acaagcttcg cgtctttgag cgcctgaacc acgaccgcgc
     3481 tgtctgtgat caacgggaga ggagcgttcc cgttgatgat tgcataacca gttacggtcg
     3541 tcggtgtgcg ctcctcaact tcgtaccgct cgcgcacact gcgcgcgagc gtgtccacaa
     3601 tcggcaactg gcggatggtg actgcgttcg agtccagata ccccgcatcc gcgtatgcgg
     3661 tccggagagc gacgaagttt tcggcagcac cggacgggga gaagaatttg ccggcgttgt
     3721 gccgtttccg gaactccttt gccagcgcgg cggtatcgag actggtcact cctgaaaact
     3781 caatggagtg gagggcgtaa cgctcctctg gagtgatacc tcctatcacg atgagcaaat
     3841 tgacgtactg aagtccctcc agtcgcccac ctaccggttc ctgcgcggtt gtggcgacgt
     3901 acgcccccag atacccgaga ttgcgatagt acgtgaggac gctcatcgta tctgccgcaa
     3961 acagtacgtc gtcgaatcgg accggcagcc agaggttccg aaatgacgga gtggggtgct
     4021 gcagaatgcc gttcagcttg cgccgctcag gaacctgtcg ccatttctcg tcgcctacgg
     4081 gagccgggac ggtagtggag gtgatgatga tctcctgcac gaaggtggac cgtgcggccc
     4141 caaccgcctg ggacatcgaa agaattgcca gcgccatcaa agcggcaaac cgtgctatcc
     4201 gcttcgtttc ccgtttccgc acttcgttcc tttccccgtg cgttacgcag cgtgcgtcac
     4261 gcagcccgtt accggaagcg tacgcggagc tgaagaccgg aggtgagttc gttttgcagg
     4321 tacagatccg tgctcgcctg tgcctgttgc agtctgttct ggccgaccgt gagcgaaagt
     4381 gactcaaggt tgggccatcg aggaggacgc tcgagtatgg tccaacgcac ttcggcgcgt
     4441 tcaagcccgc tcacgctgga taactgccct accggtgata ccgccgagaa cgtgagcttc
     4501 tggccccaca cgcgaacgtt ctgcccgact tccacttcga catcgttcat tccgccggta
     4561 ccctgttgcg tgccattgtc agcagattcc cgcacggtga cctgatctat cggcaacaca
     4621 cttcccagtg cggaggataa ggtggcgtac ccccgctcct tcagcattgt gttgagtcgt
     4681 ccctgggcat cgattgcgag gtaatcaggg ctgcgctgac cgtaaatgag aatctgcatg
     4741 atctcgagct ggtttacgat tacttcgccg cttgggagct cttccgccgt catttccgtt
     4801 ttcacgagat tcggcacgac acctgaggcg tgaagcgtta cgtcatacga cggcgcattg
     4861 ttgtccttgg tgttcaccac cttcgcgcgt gcggtcacct cgatttcagg cgcaaaacgc
     4921 gtcggatccg ggaaattgac cgtcgcctcc tgaatgacga agtcgcggcc gagatagcgg
     4981 aacaccgacc cgggggaaat ctgtacgttg ccctgaaggt tcgggctgta atgcctgccc
     5041 gtaaggttca tttgcgccca cagagtcgcg tgtttgctca gattgttgtt gatgacaagc
     5101 gaatcctcca tggagagcga gatgttgagt tccgtgttgt ccagcaacgg cccaaggctt
     5161 gtgggcgctc gcggcttgaa aatcgcctga ggatccgcga tcgggtactg gagcgttgag
     5221 ctgtacagca ccacatcggc gttcagattg gagtgctcca ttgtgccccg ccagcttcca
     5281 gtcgcgctgg cggtgaatac gcctatgggt tcggtgtaat acgcgcgcag aaggggctcc
     5341 agtaccccct tgaaaaggcg ccagcgagga ggaagaaaaa ccggttctgt acttaccagg
     5401 ctgaatgttg ccctttcaat tttcgcctca aggatgaatt cgccgcggct gagcgaatac
     5461 tcggcccgtt ccagggaaat ggccggaggg gtttgtgtct cccgctgctg tgccccgaca
     5521 aacacccgta cgggcggcac tacagcccgt tttccggtga cgcgtatgct caacgtatcg
     5581 ggcagccata tcggttcctg gagcgcctca atctccagat acgccccaaa aagctgcgca
     5641 ttgccaaaca gaaggggaaa ttccaaagtc cctcgaagct cgaactcggc agccatcggc
     5701 aacgttgccg catacgggtg cttcatggct cctctccgcg cccgaacgag ccggtcaaac
     5761 gttgtcagag gagcgttcac cgttgctccg cgcgcttccg cgggcaatgc tcgaatttgt
     5821 tgggggaatc cggggaacgg tgcagttgtc ggaagcggcg gtggtggctg gtacgtccgc
     5881 agcggtggca acaggtcggt aagcacggga accagttcga ggttggccgt gtacgcgtgg
     5941 aagaggagtg tgtccgccga aaggtccaca aatccggtca cgttgagcgt gtcgccggca
     6001 gtgtaaaaat tcgccgattc aacccacact cccgaatccc tcaccgcggc aacgcaatcg
     6061 agcgaatcac ctctcaggga gtacgccgtc ggttcgcgta tcaagaaact catgtccacg
     6121 cgaggagaac tcaggttcat gtcccaccgg gcgtgcccgg agaactcgcc ggaaaggtct
     6181 tccggtacag acgtctgcgc atcgagtgca ttgagaaggg gaaggattgt atggcggaaa
     6241 tcctccagcg atacccggtt gatactgacg tccaacgggg gacgggccac ccccggaggc
     6301 tggctgattg atatttcgcc cgtctccgcc ccgcggtagg acattgccaa atccagcggc
     6361 gatgcaaggt accggctctc tccgattgcc acggtagccg gccgtttggt gcgcaccaga
     6421 cggttgaacg gcgccacggc ggccagggaa tccagccgga acgaaccgcg gtacgacgag
     6481 atatcggcca gatcccgtcc tcgaagagcc agttcgcccg aaatacttac cgttccgctc
     6541 cgaagtccct cccagtttgg aactaccgcg ccggcatctt tcatcacgag actgagcgca
     6601 aagtccggcg ggccggtggc cgtgtctgcc agcccgagcg cactcgccgt cgcacggaat
     6661 gaccccctgg cttcgccgaa cgggaagcgg aatgagaact ccggcacgct caactggtta
     6721 gagctgtagg tggcgagcgc gcccgccgag tcaatctcca cggtcttcca cctgatacct
     6781 cggaaggtga ctctcccgtt cgcaacgggc tcctcgagag ttccgccaag atgcgcacgg
     6841 agcaccattt tcccgctgat tgccagtttt ccgtcggtca aagtgctgat cgcattgccg
     6901 acatcgaaac tgtcgagcgc ggcgcggaaa ctcatctcac cgcttcgcga gatgctcccc
     6961 tgtaaatgga gccgctggtc gttggagcct cgaaacagag tcggtgtgag gtccagaacg
     7021 cccttttcca tggagaaccg cgacggggga tccgcaagct cgtacgttgc ctgccccagc
     7081 ctgaggctgg ctcgatccag ccgtgcctga atgactaggc tgttgacaac gagccgcgtc
     7141 caccgtgccg aaatcgtacc gtcggtcatg cccgagattt tgcctgcggg ctggtcggtc
     7201 agcgccgaaa ggatccctcc aagatcgaaa ttttcccatt ggacgtctat ctgcgacgta
     7261 gcaccggagt ccgccgccac tcttccctgc gcggaaaacg tgtcgttgcc gtttcgcacg
     7321 tccagagtga gcgagttgcc gaaccagttc aaatcccccc gtatcgttcc gatctgctgg
     7381 ccgtgccatg cgacgggagt ggtgcgtatc gagccccgga ggtaagggtt cgtcagcagg
     7441 ccggccccga cgatatccgc gctcagggag ccggtgatgt cctgttcctt cgcgaacccg
     7501 ccgggcaatt gctcgaaggg aagcggacca atggatgctc tcagggacgc gaagcgcatg
     7561 aatttgttca caataacggc atccgccctg acgacgctgt ctacgcttgt caatgtggcc
     7621 gaaacggta
//
LOCUS       DAOTNG010000141         7568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8564_length_7568_cov_5.788367, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000141 DAOTNG010000000
VERSION     DAOTNG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8564_length_7568_cov_5.788367"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..856
                     /locus_tag="P5179_07965"
     CDS             188..856
                     /locus_tag="P5179_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95191.1"
                     /translation="MAKKKSQPSPEQLQRMRDTALQIEVWAKKPWRWIRDCCVTQDEA
                     DSSSPVKPFPDKEYLRYIADLWQRSSLLAIPKSRRMMLTWLMLALHLHKALFFPRSAI
                     FIQSKKEDDSDFLLSDKRMLFIYENLPKNLPWPKVTRKFCSLEFSNGSYMRGIAQGPD
                     QLRQYTASAILCDEMAFWDKAEQTWGALKPTVQGGGQVTMISSAGPGFFQRIVEGRLT
                     DDRR"
     gene            843..1673
                     /locus_tag="P5179_07970"
     CDS             843..1673
                     /locus_tag="P5179_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95192.1"
                     /translation="MTGGEINHIPIPGLKQWTTPDGIVVLQVHYSADPDKSAPEWKER
                     EMKGLSPQQWEREYEINFNAPEGKAFFPEFDMTRHVASGEMEPFVGKPVVRGWDFGLS
                     PATLFAQWGPTGQLCIFHEIQSWDCGIRAHGAVVKADSSSLFPGSRFVDYADPAGMQR
                     AQTDEKTCFQLLRLEYGFVLFPGPVSAVARSEAIRKLLTTTTPNGKPMLLVDPRCAWL
                     IAALAGGYHRREIGGRYTEDPVKDDYSHIIDCLGYVAAGQGRSDARDEPIKYPDADVF
                     "
     gene            1769..4972
                     /locus_tag="P5179_07975"
     CDS             1769..4972
                     /locus_tag="P5179_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95193.1"
                     /translation="MGVIDEMIEIMEMSMEVVPEGKDEPESEEERLERALAVVKGDRE
                     ASEELQETLAPFREEAYRFYRGRALGNERAGRSKVVSSDVMDAVEWVMPSLMRIYFSS
                     DIVSCEPVGPEDQIVAERVSALLNYQFTRRGDGFVVAYKWFKDALIYGLGVAKISWED
                     RFRDVPFAVPEMSESDFNALSSEDGVEITGFERVEIPPDEVSVQRVVQQTVMGLPPDM
                     PDDEKAEAVRMAVESMPRLATYFNVEGKRAILDYSGPVYEVIPPEDFLYDPEAEELRD
                     ARFVIHRVFRTPDYLRRMESEGVYFNVEEAIEKGNTQRDGDRSDREKGFRNAENNRTN
                     PWTMTENIEDADKRRPLELYEWWGLFDPDGSGRLTPHVITVANDVVIRLERNPYDHGE
                     PPFEVLRPVLDVHKFEGIGFADMVKEFQETKTSLRRQILDNISWQNNGMWEVQRGAGV
                     EMESLVNPRPGGVVRTDMPGAVRPLTPPPLQQAGFMALEFEQTQLEQRTGITRYNQGL
                     DSRSLNKMLALDTLIPLIDGSVKLNKDIVEGDIVVGSNGKGTKVIKAHPVQVPERAFE
                     IVFQTGDVIRSGGEHRWAVKVCGEKYQNMSPDWEKLPAERIFDLMQSGHHVYVPRVQS
                     PDFTEKELPMDPYVFGAWLGDGNSHTNRFTTQDAEILDAFEKWAKGFYKGGVEKCSKQ
                     HSGKAITYQVVNTPFRKILKDLGCLKDSRYEDTKYNVKHIPEIYLRGSFEQRLALLRG
                     LMDTDGHISKNGDSIFCSSEPALVESFVKLIETFGCKADVNWRETCGHKFPNAKPAAH
                     VVFATPFCPVTLPAKVARWRTREKFWEKQRIVSIREIPVEPMRCLSVEADDELYCCGQ
                     KMTVTANTATGITAIMGASQQRIELIARLFAETGVRNLFVKALSLNRQFVRDEFVVRL
                     YGEPIVINKDDVSGQFDILVSVGISASKQEVVQQQMIQLIQMAPGLAQAQVMTPDNIY
                     AIMVKLLEGWGFKDHSQLMTNPNVMGQMTQQMQQMQQQMQMMQMILQHPSIANVVPQI
                     AQQVQQAQAQQAQAAPPAQGEQPPAQGPVPMQGGAPV"
     gene            4969..5229
                     /locus_tag="P5179_07980"
     CDS             4969..5229
                     /locus_tag="P5179_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95194.1"
                     /translation="MMHRSLPGDNKLDPVEFLDITSRLGFEALHELAAVYLDVKYAAL
                     LSSPSGDDMTKYSLARAQGVREFVRWISETRDEVRELMERKV"
     assembly_gap    5247..5346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5513..6595
                     /locus_tag="P5179_07985"
     CDS             5513..6595
                     /locus_tag="P5179_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95195.1"
                     /translation="MEKELDQVLAQENESDTGEFEKSREEYYQSMAARARGDEDASEE
                     DESVLDEFFGEERDEQSEQGKVPDAIEEPLRASEQPPSVETPPLAQKPPLMKLKVDGQ
                     EISIHDYNDAVTLAQKGLHYTQQMQALAPYRHVLKALEANPDLQEELLNRIRGGQPAK
                     KDEKVSPAEPEKKIEIPPMREDETYEEWTQRVFKEEIPNLVRSEAERIAGAKAAEIFK
                     TENAMLEEVRRREKILDAARADPLFNQTAQFIRQSIEAGQIPPTVLQAADSDPKTFVW
                     LYDSARKKVASMMAPAQTAPVPPVAPAAPAETQRSPQPRAPHAEAASGRARPSKGSRD
                     YVKVIEDMEDAKFKELLERVKAGGAR"
     gene            6651..>7568
                     /locus_tag="P5179_07990"
     CDS             6651..>7568
                     /locus_tag="P5179_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HRS95196.1"
                     /translation="MKAADFSRAALTFYEKTLLTTSRSLLTYAQFGEKFPMKDKSGKT
                     INFARYADLSAATTELGEAITPVGSKILKSEITATLRQYGDWVGISDVTTMTGFDPEI
                     TIAAEKLGRQQGLTLDTVLRNELMQGTNEVFANSKTSTATIKDKIQKADFQKIHRFLR
                     SNDVPMITKMIDPSTGIATQPVPAAYIAIGHTDMITDLEDLEASGGFVPVHKYASSRG
                     VFEGEVGTLAGIRFILTSNAPIAYAAGDTPSAGIQASVNASKTDVYSTIIFGEGAFAE
                     VPLNKGNSGIIIKAKGAKDTMDTSDPLNQR"
BASE COUNT         1640 a         1882 c         2536 g         1410 t
ORIGIN      
        1 ttttttatgc ctcaaaaccc ccgcgaaaac gcccctagaa tcaacgagat tggaaaagag
       61 gtatggatat accttttctt gaaacttgtt cgttctaggg ccgtttctga cggtctaggc
      121 tcgatgtaac ggatatattc gtgtcggatg acactgtgtc tatgagagta aggcaggtgg
      181 ttgtttcatg gcgaagaaga aatctcagcc gtctcccgag cagcttcagc ggatgcggga
      241 tacggcgttg cagatagagg tgtgggcgaa gaagccgtgg cggtggattc gggattgctg
      301 cgtgacgcag gacgaggcgg attcgtcgtc gccggtgaag ccgttccccg acaaggagta
      361 tctgcgatac attgcggatt tgtggcagag gagttcgctg ctggcgattc cgaagagccg
      421 ccggatgatg ctgacgtggc tgatgctggc gctgcatctg cacaaggcgc tgttcttccc
      481 ccggagcgcg atcttcatcc agtcgaagaa ggaggacgac agcgacttcc ttctgtcgga
      541 caagcggatg ctcttcatct acgagaatct gccgaagaat ctcccgtggc cgaaggtgac
      601 gcggaagttc tgttccctgg agttctcgaa cgggagctac atgcgcggca tcgcccaggg
      661 gccggatcag ttgcggcagt acacggcgtc cgcgattctg tgcgacgaga tggcgttctg
      721 ggacaaggcg gagcagacgt ggggcgcgct gaagccgacg gtgcagggag gcgggcaggt
      781 gacgatgatt tcgtccgccg ggccggggtt cttccagcgg atagtggagg ggaggcttac
      841 cgatgacagg cggtgagatc aaccatatcc ccatccccgg cctcaagcag tggacgacgc
      901 cggacggaat tgtcgttctc caggtccatt actccgcaga cccggacaag tccgctccgg
      961 agtggaagga gcgggagatg aaggggcttt ccccgcagca gtgggagcgg gagtacgaga
     1021 tcaacttcaa tgctccggag gggaaggcgt tctttccgga gttcgatatg acgcggcatg
     1081 tggcgtccgg ggagatggag ccgtttgtcg ggaagccggt cgttcgcgga tgggacttcg
     1141 gactttctcc cgcgacgctg ttcgcgcagt gggggccgac gggacagctc tgcatctttc
     1201 acgagattca gtcgtgggac tgcggtatcc gcgcgcacgg ggccgtggtg aaggcggatt
     1261 cgtcgtctct ctttcccggt tcccgcttcg tcgattatgc cgatcctgcg gggatgcaac
     1321 gggcgcagac ggacgagaag acgtgctttc agctattgag gctggaatac ggattcgtcc
     1381 tctttccggg gccggtgtcg gcggtggcgc ggagcgaggc gatacggaag ctcctgacga
     1441 cgacgacgcc gaacgggaag ccgatgctgc ttgtcgatcc gcgatgcgcg tggctgatcg
     1501 cggcgctggc gggagggtac caccggcggg agatcggcgg gcggtacacc gaagacccgg
     1561 tgaaggacga ctacagccat atcatcgact gcctcggata tgtcgcggca gggcaggggc
     1621 ggagcgacgc ccgcgacgag cctatcaagt atccggatgc ggatgtgttt tagaggttgt
     1681 aagacggact cgccaaacgg cgggacgcat acctcgtgga accgggaagc ccagcctttc
     1741 taaggctggg tagctcactc gaaaggaggt gggggttatc gacgagatga ttgaaatcat
     1801 ggagatgtcc atggaggtcg tccccgaggg gaaggacgag cccgagtcgg aagaggagcg
     1861 ccttgagcgc gcgctggcgg tggtgaaggg cgaccgcgag gcttccgagg agttgcagga
     1921 gacgctggct ccgttccgcg aggaggcgta ccggttctac cgggggcgcg ccctggggaa
     1981 cgagcgggcg gggcgctcca aggtggtgtc ttccgacgtg atggacgccg tggagtgggt
     2041 gatgccgtct ctgatgcgga tttacttctc cagcgatatc gtgtcgtgcg agcccgtggg
     2101 gccggaggat caaatcgtgg cggagcgggt gagcgcattg ttgaactatc agttcacgcg
     2161 gcgcggcgac gggttcgtcg tggcgtacaa gtggttcaag gacgcgctga tctacggtct
     2221 cggcgtggcg aaaatatcgt gggaggaccg tttccgcgac gtgccgttcg ccgtgccgga
     2281 gatgtcggag agcgatttca acgcgctttc gtcggaggac ggggtggaga tcaccgggtt
     2341 cgagcgggtg gagattccgc cggacgaggt gtcggtgcag cgggtcgtgc agcagacggt
     2401 gatgggattg ccaccggaca tgcccgacga cgagaaggcg gaggccgtgc gcatggcggt
     2461 cgagtcgatg cctcgtctcg cgacgtattt caacgtcgag gggaagaggg cgattctcga
     2521 ttattcgggg ccggtgtacg aagtgattcc gccggaagat tttctgtacg atcccgaagc
     2581 ggaagagttg cgcgacgccc gcttcgttat ccatagagtt ttccggacgc ctgactacct
     2641 gcggcgaatg gagtcggagg gcgtgtactt caacgtcgaa gaggccatcg agaaggggaa
     2701 cacgcagcgc gacggcgaca ggagcgacag ggagaagggc ttccggaacg cggagaacaa
     2761 caggacaaac ccgtggacga tgacggagaa tatcgaggac gcggacaagc gtcgtccgct
     2821 ggagttgtac gagtggtggg ggctgttcga tccggacggg agtggacggc tgacgccgca
     2881 cgtcatcacc gttgcgaacg acgttgtgat tcgtcttgag cggaacccct acgatcacgg
     2941 ggagccgccg ttcgaggtgc ttcgtcctgt tctcgatgtg cacaagttcg aggggatcgg
     3001 cttcgccgac atggtgaagg agttccagga aacgaagacg agccttcgca ggcagatact
     3061 cgacaacatt tcgtggcaga acaacgggat gtgggaggtg cagcgcgggg ccggagtgga
     3121 gatggagtcg ctggtgaatc cgcgtccggg cggcgtcgtc cgtaccgaca tgccgggggc
     3181 cgtgcgccct ctgacgcccc ctccgttgca gcaggccgga ttcatggcgt tggagttcga
     3241 acagacgcag ttggagcagc gaaccggtat aactcgttat aatcaggggc tggatagtcg
     3301 cagcttgaat aagatgctcg ctttagatac gcttattccc ctgatagatg ggtccgtgaa
     3361 attgaataaa gatattgttg aaggggacat tgttgtcggg tccaacggta aggggacaaa
     3421 agttatcaag gcgcatccgg tgcaggtacc ggaacgtgca ttcgaaatag tgtttcagac
     3481 tggggatgtt atcaggtcgg gtggagagca taggtgggcc gtgaaggtgt gcggcgagaa
     3541 atatcagaac atgtctcctg attgggagaa actccctgcg gagcgcatat tcgatcttat
     3601 gcagtccgga catcatgtct atgtgccgcg cgtgcagtct cccgatttca ctgaaaaaga
     3661 gttgccgatg gacccgtatg tgtttggcgc atggcttgga gacggcaatt cgcatacgaa
     3721 tagattcacg acgcaggatg cggagattct cgacgcattc gaaaaatggg caaaggggtt
     3781 ctataagggc ggcgtggaaa aatgctcgaa gcagcacagc gggaaagcta ttacatatca
     3841 ggtggtaaac accccgttcc gtaaaatact caaagacttg gggtgtttga aggattcaag
     3901 atacgaagat acgaaatata acgtgaagca cattcccgaa atatatcttc gcgggagttt
     3961 tgaacagcgt cttgcgctgt tgcgcggcct gatggatacg gacggacaca tcagcaagaa
     4021 cggggattct attttttgca gttctgaacc ggcgctggtt gagagctttg tcaagctcat
     4081 cgaaacattc gggtgcaagg ctgacgtgaa ttggagagag acgtgcggac acaagttccc
     4141 gaatgcgaaa ccggctgcgc atgttgtttt tgctacgccg ttctgccccg tgacacttcc
     4201 cgcaaaggtc gcgcgatgga gaactcggga gaagttttgg gagaagcagc ggattgtttc
     4261 gataagagaa attcctgtcg aaccaatgcg gtgtctttct gttgaagctg atgacgagtt
     4321 gtattgttgc ggtcaaaaaa tgacggtgac ggcgaatact gccacgggca taacagcaat
     4381 aatgggggcg agccagcagc gcatcgagct gattgcaagg cttttcgccg agacgggtgt
     4441 aagaaacctc ttcgtcaagg cgctttcgtt gaaccggcag ttcgtgcgcg acgagttcgt
     4501 cgtgcggctg tacggagagc ctatcgtgat caacaaggac gacgtgtcgg ggcagttcga
     4561 cattctggtg tccgtcggca tttcggcgag caagcaggag gtcgtgcagc agcagatgat
     4621 tcagcttatc cagatggctc cgggtttggc gcaggcgcag gttatgactc cggacaacat
     4681 ctacgccatc atggtgaagc tgcttgaggg atggggcttc aaggatcaca gccagttgat
     4741 gacgaatccg aacgtgatgg gacagatgac gcagcagatg cagcagatgc agcagcagat
     4801 gcagatgatg caaatgatct tgcagcaccc gagtattgcg aacgtggtac cgcagatagc
     4861 gcagcaggta cagcaggcgc aggcacaaca ggcgcaggcg gctcctccgg cacaggggga
     4921 acaacctccg gcacaggggc ctgtaccgat gcagggaggt gcgccggtat gatgcaccgg
     4981 agccttcccg gagacaacaa gctcgatccg gtggagttcc tcgacataac gtcgaggctt
     5041 gggttcgagg cgctgcacga gcttgcggcc gtgtatctgg atgtgaagta cgccgcgctg
     5101 ttgagttctc cctccggcga cgacatgacg aagtattccc tcgcgagggc gcagggtgtg
     5161 cgggagttcg tccgctggat cagcgagacg cgcgatgaag tcagagagct gatggagcgg
     5221 aaggtatagc gagagcaaca aggggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnagcg agatcaacaa aggagagacg ggatagcccg tctttttcca tatatggggg
     5401 cttcggagcg atggcttcgg agcccttttt tattggccaa cccggagagg gggccgcccg
     5461 cgttcggggg tatccgccgg tttccgggag cacaaaacaa ggaggcacta ccgtggagaa
     5521 agaactggat caggtactgg cgcaggagaa cgaaagcgat accggcgagt tcgaaaagtc
     5581 gagggaggaa tactaccagt cgatggccgc acgcgctcgc ggagacgagg acgcgtcgga
     5641 ggaagacgag tcggtattgg atgagttttt cggcgaagag cgggatgaac aatcggagca
     5701 gggaaaggtt ccggatgcaa tagaagaacc gcttcgagcg tcggagcagc ctccttccgt
     5761 tgagacgccg cccctggcgc agaagccccc gttgatgaag ttgaaggtgg atggacagga
     5821 gatttccatt cacgactaca acgacgcagt gacgctggcg cagaagggat tgcactacac
     5881 gcagcagatg caggcgctcg cgccgtaccg gcacgtattg aaggctctcg aagcgaatcc
     5941 tgatttgcag gaggagcttt tgaaccggat tcgcggagga cagcccgcga agaaggatga
     6001 aaaggtttcg ccggcggagc cggagaagaa gatcgaaata cctcccatgc gggaggatga
     6061 aacctacgag gagtggacgc agcgcgtctt caaggaggag attccgaatc tcgtgcgttc
     6121 ggaggcggag cgtatcgccg gtgcgaaagc ggcggagata ttcaagaccg agaacgcgat
     6181 gcttgaagag gtgcgccgca gagaaaagat tctcgacgcg gcgcgtgccg atcctctttt
     6241 caaccagacg gcgcagttca tccggcagtc gatagaggcc gggcagattc cgccgactgt
     6301 gcttcaggcc gccgacagcg atccgaagac attcgtatgg ctgtacgaca gcgcgcgtaa
     6361 gaaggtggcg tcgatgatgg ctcccgcgca gacagcgccg gttcctccgg tcgctcccgc
     6421 agcgcccgcc gagacgcaga gaagtccgca gccccgcgcc ccgcacgccg aagcggcgtc
     6481 cggacgggca cgcccgtcga agggttcgcg cgattacgtg aaggtgatcg aggacatgga
     6541 ggacgcgaaa ttcaaggaac tgctcgaacg agtgaaagcg ggtggcgcca gatgaggcgg
     6601 gtacccgctc aaattttatc agggagtgat actacttgag cacacagtat atgaaggcgg
     6661 ctgatttttc cagagcggca ctgacgtttt acgagaagac gcttctgacg acatcgcgtt
     6721 ctcttctcac ctacgcgcag ttcggtgaga agttcccgat gaaggacaag agcggcaaga
     6781 cgatcaactt cgcccgctac gccgatcttt cggcggcgac gaccgaactt ggcgaggcca
     6841 tcaccccggt cggcagcaag attctcaaga gcgagatcac ggcgactctt cgccagtacg
     6901 gcgattgggt cggtatctcc gacgtgacga cgatgaccgg tttcgacccc gagattacca
     6961 tcgccgccga gaaactcggt cggcagcagg gtcttacgct cgacacggtg ctccgcaacg
     7021 agctgatgca ggggacgaac gaggtgttcg ccaacagtaa aacgtccacg gcgacgatca
     7081 aggacaagat acagaaggct gacttccaga agattcaccg cttcctgcgc tccaacgacg
     7141 tgccgatgat caccaagatg atcgacccga gcaccgggat tgcgacgcag cccgtgcccg
     7201 ccgcgtacat cgccatcgga cacacggaca tgatcaccga tctggaggac ctggaggcga
     7261 gcggcgggtt cgttccggta cataagtacg ccagcagcag gggcgtgttc gagggcgagg
     7321 tcgggacgct tgcgggtatc cgcttcatcc tcaccagcaa cgcgccgatc gcctacgccg
     7381 cgggcgacac gccgagtgcc ggcatccagg cgagcgtgaa cgcttcgaag acggacgtgt
     7441 attcgaccat catcttcggc gagggcgcgt tcgcggaggt tcctctgaac aaggggaaca
     7501 gcgggatcat catcaaggcg aagggcgcga aggatacgat ggataccagc gacccgctga
     7561 accagagg
//
LOCUS       DAOTNG010000142         7539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8600_length_7539_cov_4.946553, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000142 DAOTNG010000000
VERSION     DAOTNG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_7539_cov_4.946553"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5179_07995"
     CDS             complement(<1..219)
                     /locus_tag="P5179_07995"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRS95197.1"
                     /translation="MSPEEISRLPVRRIVGLMSGTSVDGIDAALVEISGHGASLRLER
                     VRAAETYSYPREVQEQIHALFDGHVRELC"
     gene            complement(232..1389)
                     /locus_tag="P5179_08000"
     CDS             complement(232..1389)
                     /locus_tag="P5179_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95198.1"
                     /translation="MPPTVTNSQALRRLALSLANWSEAPPRVLLRRGDSVRFAHAIAL
                     LGLQLMQTASESETSPAGSSRESSVLVRDGFAERVQTMVSLADRERSATLAEELVNEA
                     VESLTALGTAATSLRFAEGAVRGLPLTPREVDASAEAFFVRAKPLGAVAVLVADSYAR
                     GCADRASDLDVRLLAHEIPDPRSRLRAFAPLADRGIVRQYPGDDIIHADQMVIAGRRV
                     DLRFHTIRSLEVALQGPFIPGGAIEALELLQTNQIILDPQGIATGLVRRSDLLAQRAR
                     EIATVSLSALREWDEATVKSAGSAAEAMVRTLGPGLEYLIRAWAGLNGRVHAFPRWVD
                     AAVLPLPVSPRDGRRVLQRLVTSPWTAKDVPARLSEWKTLSLALSELDHPL"
     gene            complement(1492..2139)
                     /locus_tag="P5179_08005"
     CDS             complement(1492..2139)
                     /locus_tag="P5179_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRS95199.1"
                     /translation="MGGAGEILRRAAARYRDALAIVDDYMERETARHPEQVRCRRGCA
                     ECCFGTFAFSLADMALVQSAFGSLNEEIQEYCVRRATAFVENMGEVAARRVDLLDEAE
                     LDRLLESVAALPCPFLSPEHDCRIYRSRPLVCRIHGLPIKGNGNELLGPGCRLNFVGI
                     RVEELSGYQLDIAEFDVAEATEIAGIAEALGVDEETQILLPAAILPPWFLPSDEK"
     gene            complement(2147..>2974)
                     /locus_tag="P5179_08010"
     CDS             complement(2147..>2974)
                     /locus_tag="P5179_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HRS95200.1"
                     /translation="RSFFHSLTRMSEQSGGRIPTFMSTHPDPGDRENTVVRLAAEWAA
                     QGYATNKVGRDTLFDHIDGMILGQNPRQGFTDGGVFYHPDLRFQFPVPNLWRTVNDAA
                     QVVLVAPDQNANIQISIVPGATTASAAAASFLSQDGVTATENTSVTVNGLPARDVRAA
                     VTVSGTALSVVAYFIEYNGKVYAFVGLTPKALAATYESSFLNTMRGFRALTNSTILAV
                     KPFRVKTVVASRTGAFSSFLPATMPPGFTANDAAIMNQVELTDTIQKGTRLKLWEQR"
     assembly_gap    2975..3074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3075..3523)
                     /locus_tag="P5179_08015"
     CDS             complement(<3075..3523)
                     /locus_tag="P5179_08015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRS95201.1"
                     /translation="MEKRTRGCTRAILALVALVTFGGCAVSVNPVTGKRGMYAYSWQQ
                     EISLGAETDVAIGQEYGLYEDAALTSYVRSVGESVLAKSHLRRPEAREEFRKTVFTFR
                     VLDSPVVNAFAVPGGYVYVTRGLLAYAENEAQLAVVLGHEIGHVAAR"
     gene            3914..>4278
                     /locus_tag="P5179_08020"
     CDS             3914..>4278
                     /locus_tag="P5179_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRS95202.1"
                     /translation="MKGLTQKKRNPSKEIDLIAFVAHELKNPLASCLMNAYMLKDGLL
                     GELNERQQKAVASLVRNLEYFDDTIHHYLNLTRIEQGNLKIEPRSILVVHDVVQPCLE
                     NLDRQITTKKIKVVNELPPG"
     assembly_gap    4279..4378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4379..4809
                     /locus_tag="P5179_08025"
     CDS             <4379..4809
                     /locus_tag="P5179_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS95203.1"
                     /translation="DRQITTKKIKVVNELPPGDIRLVADPALLRIVFNNLLNNAIVYG
                     REGGTVRLSAFEHGGWWEFSVWNEGVGIPKNKIKTLFRKFNRVENPSAPKLRGTGLGL
                     YITRTIIERHGGTISADSEEGSWAQFRFTLPAVVSPASGA"
     gene            4914..5657
                     /gene="hisA"
                     /locus_tag="P5179_08030"
     CDS             4914..5657
                     /gene="hisA"
                     /locus_tag="P5179_08030"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRS95204.1"
                     /translation="MLILPAIDLRDGRVVRLRQGNPDEQTTYSESPASAAQAWFEAGA
                     EWLHVVDLDGAFAGEPVNIAAIRSIVAAAPVPIEVGGGIRSVGTAAKLLALGVSRVIF
                     GTVAVTHPEIVGQAIERFGAERIVVGLDSKNGNVAVRGWTEASSLAVLAAARHLKESG
                     VVRVIHTDIARDGMFTGPDIDTAALLARETGLSVITSGGVGSLADIRRVAEKKELEGV
                     IVGKALYDGAFTLQAALEIAGNTSNNATG"
     gene            5676..6695
                     /gene="aroF"
                     /locus_tag="P5179_08035"
     CDS             5676..6695
                     /gene="aroF"
                     /locus_tag="P5179_08035"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRS95205.1"
                     /translation="MIVVMHRGATQEEISHVCEHIVELGFKAHPIYGTERVVIGVIGD
                     DHAKTEAMATLGIQPGVEQVTPILKPYKRVGAEMKKERTVVKVGDAAFGGGNFAVIAG
                     PCSVENEEQLLSTARKVKAAGARVLRGGAFKPRTSPYAFQGLAEEGLRLLSLARSETG
                     LPFATEVVTNRDVELIARYADMLQIGARNMTNYSLLREVGATGKPVLLKRGLSATLEE
                     LLLAAEYIADEGNEQIVLCERGIRTFEPFTRNTLDLSAVPAIHILSHLPIIVDPSHAT
                     GRSELVRSMALAAIAAEADGLIVEVHPAPEQALSDGAQSILPEAFAQLMEEGRRYLAV
                     SGRTL"
     gene            6692..>7539
                     /gene="proB"
                     /locus_tag="P5179_08040"
     CDS             6692..>7539
                     /gene="proB"
                     /locus_tag="P5179_08040"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRS95206.1"
                     /translation="MSEAISQTTQRAYDLDHSFDRRTALGNVRRVVVKVGTSTLSSEN
                     GRLDRAYIAELMEQVAGLRNSGREVAIVSSGAVGAGIGALNLKKRPGSIGDLQAVAAV
                     GQSLLMHFYDDAASPMGIRVAQVLLSAADLRRKVGYQNVRNVFESLFRLKALPIVNEN
                     DSVAVEELKFGDNDSLSAHVANLVDADLLVILSDVDGLYRGTPGAESAPPLIPTVYRI
                     ESEVERLCGASTSGVGIGGMATKISAAKMLAASGVPTIIANGRTTRLEDIVSGKPVGT
                     IFLPVSV"
BASE COUNT         1569 a         2270 c         2117 g         1383 t
ORIGIN      
        1 gcacagttca cgcacgtggc cgtcaaaaag agcatgaatc tgctcctgca cctcgcgcgg
       61 atacgaatat gtctccgccg cacgtacacg ttccaggcgc agagacgcac cgtggccgct
      121 gatttccacc agagcggcat caatgccgtc cactgaagta cctgacatca ggccgacgat
      181 gcgtcgcacc ggcagtcggc tgatttcctc tggcgacacg gcaggcacct ttcacagggg
      241 gtgatccagt tccgaaagcg cgagggacaa cgtcttccat tcgctcaggc gcgccggaac
      301 atctttcgct gtccacgggc tcgtcacgag tcgctgaagg actctgcgac cgtctcgcgg
      361 cgagacgggt aacggcagca ctgcggcatc cacccatcgc ggaaacgcgt ggacacgacc
      421 gttcaacccc gcccaggcgc gaatcagata ctccagcccc ggcccgagag tccgcaccat
      481 agcttccgca gcagacccgg cggacttcac agttgcctca tcccattccc gcaacgcact
      541 cagcgagacg gtggcgattt cccgcgcacg ttgtgcgagc agatcggaac ggcggacaag
      601 gccggtggct atcccttggg gatccagaat gatctggttg gtctgaagca attcaagagc
      661 ctcaatcgct ccacccggaa tgaatggccc ctgcagcgcc acttcgagcg accggatcgt
      721 atggaaccga aggtccacac gccgccctgc gatcaccatc tgatccgcat ggataatgtc
      781 gtcccccggg tattgccgga ctatcccgcg atccgccagt ggcgcgaatg cccgaagcct
      841 gctgcgagga tccgggatct cgtgtgcgag cagccgcaca tccaggtccg atgcgcggtc
      901 ggcgcacccg cgcgcgtacg aatccgctac gagaaccgcc accgccccca gcggcttcgc
      961 gcggacaaaa aacgcctctg ctgatgcatc cacctcgcgc ggcgttaacg ggaggcctcg
     1021 aaccgcgcct tcggcgaatc gtagtgacgt tgcggcagtc ccgagggcgg tcagtgactc
     1081 cacggcctcg ttcacaagct cttccgccag agttgccgaa cgttccctgt ctgcaagcga
     1141 taccatcgtc tgcacgcgtt ccgcgaagcc gtccctcacc aacaccgagg actcccggga
     1201 agacccagct ggtgaggttt ccgattcgct cgctgtctgc atgagctgga gcccgaggag
     1261 tgcgatcgcg tgggcaaaac ggacggaatc ccctcttctc agaagcacgc gcggcggggc
     1321 ttcgctccag ttggcaagtg agagagccag tcgcctcaaa gcctgagaat tcgtcacagt
     1381 agggggcatc gcgaatacac gttcttctgg cgcggtctca gcgggatcaa tctggatgcg
     1441 gttcggattt ccggaaacgc gagacgtgcc gcgctttgcc gttctccatt ctcatttttc
     1501 atcagacggc agaaaccagg gcgggagaat agccgccggg agaaggatct gtgtctcctc
     1561 gtcaacaccg agcgcttcgg cgatccccgc gatctcagtc gcttccgcaa cgtcgaattc
     1621 ggcgatgtca agctgatacc cgctcagttc ctcgacacgt attcccacga aattgagccg
     1681 acacccgggg ccaagaagct cgttaccgtt cccttttatg ggcaagccgt ggatcctgca
     1741 taccagaggg cgcgatcggt aaatgcggca gtcgtgttcc gggctcagaa aagggcacgg
     1801 caacgccgcc acggattcaa gaagccgatc cagctctgcc tcatccagaa gatcaacccg
     1861 acgcgccgca acctcaccca tgttttcaac gaacgcggtc gcgcgccgaa cacaatactc
     1921 ctgaatttcc tcgttcagac tcccgaacgc ggactgcacg agagccatgt cggcgagtga
     1981 aaaggcaaac gtcccgaagc agcattccgc acaaccacgc cggcaacgaa cctgctcagg
     2041 gtggcgagcg gtttcacgct ccatgtagtc atcgacgatg gcaagggcat cccggtacct
     2101 cgctgccgcc cgccgcagaa tctccccggc accacccacc ggcaaactaa cgctgctccc
     2161 aaagtttgag ccgggtaccc ttctgaatgg tatccgtaag ctccacctga ttcatgatcg
     2221 ccgcatcgtt cgccgtgaac cccgggggca tcgtggcggg cagaaacgag gaaaacgctc
     2281 ccgttcggct tgccacaact gttttcaccc ggaacggctt cacggcaagg atcgtggaat
     2341 ttgtgagcgc gcggaaaccc cgcatcgtgt tcaggaagct cgactcgtac gtcgcggcca
     2401 gcgccttggg cgtgagtccg acaaacgcat ataccttccc gttgtattcg atgaaatacg
     2461 ccaccaccga cagcgctgtc ccgctcacgg taaccgcggc ccgcacatcc cgcgcaggga
     2521 gaccgttcac cgtaacactc gtgttttccg ttgcagttac gccgtcctgg gaaagaaagg
     2581 aggccgcagc ggccgaagcc gtcgtcgcac ccggaacaat ggaaatctgg atgttggcgt
     2641 tctgatccgg tgcgacaagc accacttgcg ccgcgtcatt cacggttcgc cacaagttcg
     2701 gaaccgggaa ctggaaacgg aggtccggat ggtagaacac acctccgtct gtgaaaccct
     2761 ggcgtgggtt ctgacccaga atcatcccat caatgtggtc aaaaagcgtg tccctgccca
     2821 ctttgttcgt agcgtaaccc tgtgccgccc attccgctgc cagcctgaca accgtgtttt
     2881 cgcgatcccc cgggtccgga tgtgttgaca tgaacgtcgg tatccgtccg ccggattgct
     2941 cgctcatccg cgtcagactg tgaaaaaacg accgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnntgccgg gccgccacat ggccgatttc atgcccgaga acgacggcaa
     3121 gctgcgcctc gttctccgca tacgcaagga gtccccgcgt tacgtacaca taacctccgg
     3181 gaactgcaaa ggcgtttacg accgggctgt ccagcacccg gaaggtgaac accgttttcc
     3241 tgaactcctc cctcgcctcc ggacgccgga gatggctttt cgcaagaacc gactcgccga
     3301 cgctccgcac gtacgacgtg agcgccgcat cctcgtaaag cccgtactcc tggccaattg
     3361 ctacgtcggt ttccgcaccc aggctgatct cctgttgcca ggagtacgcg tacatgcccc
     3421 gttttccggt aaccgggttg acagaaaccg cacagccacc gaacgtgaca agagcaacca
     3481 atgcaagaat tgctctggtg catccccgtg tcctcttttc cattttctcg cccttttcgt
     3541 ccacacgtat gttgtccaac gcctgcgcca tttcctaaca aatgtagccg gattcgcgct
     3601 tcaaatacat cgggcgccac aagattttgt gtctgcctgc gtgcgccttc gaacaggaca
     3661 accgtaatcg ggcttccttt tcagcggcgc gggtaatcgt ctcttgcggc aatgtcgaac
     3721 acaccaaccg gcgcggaatt tgcacctgtc tattctgaag ggtcgggagt tctgaaaaag
     3781 cgctgcggcg ttcccgacgg cgcgagggca cgcttctccg cccgcggcgg ctctcctgtt
     3841 ctcatgatgc ctgcttcggg cggaaaaagg cctgcgcaga aatggcgcac tgtgcccaga
     3901 caaggaacaa cagttgaagg gtttgactca gaaaaagcgc aatccttcca aggaaatcga
     3961 cctcattgcg ttcgttgcgc acgaactgaa aaaccctctt gcctcctgtc tgatgaacgc
     4021 atacatgctg aaagacgggt tgctggggga actgaacgaa cggcagcaga aagcggtcgc
     4081 ttcccttgtc aggaatctgg aatatttcga tgacacgatt catcattacc tcaaccttac
     4141 gcggatcgaa cagggcaatt tgaaaataga accgcgttcc atcctcgtag ttcacgacgt
     4201 cgttcaaccg tgtctggaaa acctggacag gcagatcacc acgaagaaga tcaaagtcgt
     4261 gaacgaactg ccgcccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     4381 gacaggcaga tcaccacgaa gaagatcaaa gtcgtgaacg aactgccgcc cggggatatc
     4441 cgtctggtcg cggatccagc cctgctgcgc atcgtgttca acaatctcct caacaacgcg
     4501 attgtgtacg ggcgcgaggg gggaaccgtc cgcctgtccg cattcgaaca cggcggatgg
     4561 tgggagtttt ctgtgtggaa cgaaggcgtg gggattccca agaacaaaat caagaccctt
     4621 ttccgcaaat tcaaccgggt ggaaaaccct tccgctccga aacttcgcgg caccggtctc
     4681 ggcctctaca tcacgcgcac gatcatcgaa cggcacggcg gaaccatctc cgcagactca
     4741 gaggagggtt cctgggccca gttccgattc acattgcccg cggttgtttc accggcttcg
     4801 ggggcctgat cgagcgccaa gggtggacga gttgcgcccc gtccgttata ttttcccctc
     4861 gcctcgcgtg caattgacgt tcggcgattt tccacaatcc ttcccttgct ccgatgctga
     4921 ttctgcctgc gattgatttg cgtgatggac gggtagtacg cctgcgacag ggcaaccctg
     4981 acgaacagac gacgtattcc gagagccccg caagtgcggc acaggcctgg ttcgaggcag
     5041 gtgcggagtg gctgcatgtg gtggatctgg acggcgcatt cgcaggcgaa ccggtgaaca
     5101 tcgcagcaat ccgctcgatc gtcgccgcgg cgcctgttcc gattgaagtc ggcgggggca
     5161 tacgctccgt cggtactgcg gcgaaattgc tggcgcttgg cgtttcacgg gtgattttcg
     5221 gaaccgtcgc ggtcactcac ccggaaatcg ttggccaagc aattgagcgg ttcggtgccg
     5281 aacggatcgt ggtgggtctg gactccaaga acggaaatgt tgcggtgcga ggttggaccg
     5341 aggcgagctc tcttgcggtt ctggcggcgg cacgacacct gaaagaatcg ggagtcgtcc
     5401 gggtcatcca taccgatatc gcccgtgacg gaatgttcac cggacccgat atcgacaccg
     5461 ccgccctgct tgcgagggaa accggcctgt ccgtgatcac ctccggcggc gtgggctcgc
     5521 tggccgacat ccgccgcgtt gccgaaaaga aagagctgga gggagtgatc gtgggaaaag
     5581 cgctgtacga tggagcattt accctgcagg cagcgctgga aattgcagga aacacgtcga
     5641 acaacgcaac aggatgaatt gactgagggg tacgcatgat cgttgtcatg caccgcggtg
     5701 caacacagga agaaatcagc cacgtatgcg agcatatcgt ggaactcggg ttcaaggccc
     5761 acccgatcta cggcacggaa cgagtggtaa tcggcgtgat cggcgacgat cacgcgaaaa
     5821 ccgaagcgat ggcaacgctt gggattcagc ccggcgtgga acaggtcacc ccgatcctca
     5881 agccgtacaa acgcgtcggc gcggaaatga aaaaagaaag gacggtggtg aaagtcgggg
     5941 acgccgcatt cgggggtgga aatttcgcgg tgatcgccgg tccctgctcc gtagaaaacg
     6001 aggaacagct cctttccacc gcgcggaaag tgaaagcagc gggcgcacga gtgcttcgcg
     6061 gcggcgcatt caaaccccgg acctcgccct acgcgttcca gggcctcgca gaggaagggc
     6121 tcaggctcct ttccctcgca agaagcgaga ctggacttcc gtttgcgacc gaggtagtga
     6181 ccaaccggga cgtggaactg atcgcccgct atgcagacat gctgcagatc ggcgcgcgga
     6241 acatgaccaa ctactcgctc ctccgtgaag ttggcgccac cggaaaacca gttcttctta
     6301 agcgcggatt atcggcgaca cttgaagaac tgctgctcgc ggcggaatac atcgctgacg
     6361 agggcaacga gcagatcgtg ctgtgcgaac gcggcatccg cacctttgaa cctttcaccc
     6421 gaaatacgct ggacctttct gcggtaccgg cgatccatat attgtcccat ctgccgatca
     6481 tagttgatcc aagccacgca accgggcgga gcgaactcgt tcgttcaatg gcccttgcgg
     6541 caatcgccgc cgaagccgac gggcttatcg tggaagtcca cccggctccg gaacaggcgc
     6601 tgagcgatgg tgcgcaatcg atccttcccg aggcattcgc gcagctcatg gaagaggggc
     6661 ggcgctacct tgcagtatcc gggcgcacgt tatgagcgaa gcgatctcgc agacaacgca
     6721 acgggcgtat gacctggacc actcgtttga ccggcgcacc gcgttaggca acgtcaggcg
     6781 cgtggtggta aaggtcggaa ccagcacact ctcgtccgaa aatggacgcc tcgacagagc
     6841 ttacatcgca gaattgatgg aacaggtcgc cggactgagg aacagcggcc gcgaagtcgc
     6901 gatcgtatcc agcggcgcag ttggcgcggg aatcggagct ctcaacctca aaaagcgacc
     6961 gggttcaatc ggtgaccttc aggctgtggc ggcagtgggc cagtcgctcc tcatgcactt
     7021 ttatgacgat gcggcatcgc cgatggggat ccgcgtcgcg caggtactcc tgagcgccgc
     7081 cgacctgcgc cgaaaagtcg ggtaccagaa tgtccgcaac gtgtttgagt cgctcttccg
     7141 cttgaaagcc ctccccatcg tgaatgagaa cgattccgtt gccgttgagg aactgaaatt
     7201 cggggacaac gactctcttt cggcgcatgt ggcaaatctc gtcgacgctg accttctcgt
     7261 gatactgtca gatgtggacg gattgtaccg gggaaccccg ggcgccgaat cggctcctcc
     7321 gctgatcccc acggtctacc gaatcgaatc cgaggtggaa aggctctgcg gcgcgagcac
     7381 gagcggcgtg ggaatcgggg gaatggcaac caaaatcagc gctgccaaaa tgcttgccgc
     7441 atcaggagta ccgacgatca ttgccaatgg tcgtacaacg cgcctggaag acatcgtatc
     7501 cggcaagcca gtgggaacca tcttcctgcc cgtctctgt
//
LOCUS       DAOTNG010000143         7522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8622_length_7522_cov_5.102451, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000143 DAOTNG010000000
VERSION     DAOTNG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8622_length_7522_cov_5.102451"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..683
                     /locus_tag="P5179_08045"
     CDS             294..683
                     /locus_tag="P5179_08045"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRS95207.1"
                     /translation="MVSKPEIQALADRIGKAFQPERVILFGSHAYGVPGPHSDVDLLV
                     LLPFEGRNLSKSLEILNAVNPPFSVDLLARRPDDTRRRYEQGDPLIREALDRGEVLYE
                     RSRQGMGDKSRGRFPHRKPRVPRAAKP"
     gene            595..945
                     /locus_tag="P5179_08050"
     CDS             595..945
                     /locus_tag="P5179_08050"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRS95208.1"
                     /translation="MNEVVREWVTKAEGDFRTASRECRARQNPNFDAVCFHAQQCVEK
                     LMKAALISAGLVPPKTHDLEQLDRLLKQTHTRWDVDLTDLRYLARASVAFRYPGESAQ
                     TRRKLKKPSPSARA"
     gene            complement(980..2080)
                     /locus_tag="P5179_08055"
     CDS             complement(980..2080)
                     /locus_tag="P5179_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRS95209.1"
                     /translation="MSDFASFNIRDFGAIGDGRHDDTAAIQKALDKAAGTRASVFVPD
                     GVFLSSTIKIPPHVGLIGNPTWSFRHDGGSIIRLGDPSAKCLVDLTGAIGATLNGLCL
                     DGASLAAAGSDGGIHGIFVDKKDSGDQEDTPRIERCRVNHFTGDGVRLTHIWCFSIRS
                     CMFSHNKGDGLSVRGWDGFIMDNWFSGNGGAGYAAREDNASMTMTGNRIEWNAGGGIV
                     IHGGSHYNITGNYIDRSGGAGITLMPRGGRPAHVITITGNVIFRSGAPMWRKLEDLES
                     THLRFEGVRGLVCTGNAMRVWRNDGDSGGEFSPNYGIVVKGLVNSVIKDNTLDYGAMT
                     ELLVDLGGHDENSVIRDNPGCLFVDNHWKDWD"
     gene            2265..4049
                     /gene="dnaG"
                     /locus_tag="P5179_08060"
     CDS             2265..4049
                     /gene="dnaG"
                     /locus_tag="P5179_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRS95210.1"
                     /translation="MPDDDLKERIRAAVDIVTVIGERIPLKKAGRNFVARCPFHQEKT
                     PSFNVNPERQIYHCFGCGAGGDVFSFLMEHDKVSFPEALRTLAAKAGIALPDRQSARA
                     RTNEQARDIYYRANALAREYFIAALNDPAKGEAARRYLERRGLTKDTIATFGLGFAPD
                     AWDGFMAYARPKGLSYEVLANAGLLVQNEQGRFYDRFRGRVMFPVVNDSGRTVAFGAR
                     TLDPAGEPKYLNSSESPVYQKGRILYGLYHARDAIRQENSVLIVEGYMDVLQLMQAGI
                     RNVVATCGTALTREHGAILRRYADRIILLFDGDEAGIRAASRAGDVLLEAGIEGHVAL
                     LPGGEDPDTLVSAEGPEALRSIAERGMPYLQFRWETLARQNNLATISGRNRAIGDMLD
                     VIARVDDEMLRGMMANQIAQWGVTDETLVQRAIARRRRQTVRREQEAAPPQTKRWAPP
                     HREKQLVAWMMDRPGVCQQVAEMGVERFSDPLLQEIAASLIRLKDLGELPSAAALLDE
                     KRDDSRWAGAVAELAQMHCDEREIGRAVTQLVASLQLPHLKRQIDTLRARLRVEKGIR
                     ETIEITTEIQRLGREMEILNDLLHSGEN"
     gene            4170..4559
                     /locus_tag="P5179_08065"
     CDS             4170..4559
                     /locus_tag="P5179_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS95211.1"
                     /translation="MKKQTQHTVSPVRKGLANEQVMSAMNQDREGDLLTGEQVFAVLS
                     RHKNALAERYGVNSLGVFGSMARGDSGPSSDVDIVFTTDSPNLFRSVRMKLELERLLR
                     RPVDIVRLRDNMNPRLKARILREARYV"
     gene            4552..4923
                     /locus_tag="P5179_08070"
     CDS             4552..4923
                     /locus_tag="P5179_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS95212.1"
                     /translation="MSDSDVLKARLEAVLEALQRIPRRFAAIRAPEDFEKTEAGRDNL
                     DSICMVLVASGEAFKQIDRKTNGRFLRNYPEIEWEGVMGVRDVIAHGYFDVDPDQVFD
                     ICKNDIPTLIGTVERMIADLR"
     gene            5179..5412
                     /locus_tag="P5179_08075"
     CDS             5179..5412
                     /locus_tag="P5179_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRS95213.1"
                     /translation="MRTVQLTLDENLVRDVDRLVKQRNTTRSAFTRDALQEAIKAART
                     REMEEKHRAGYRKLPVRKGEFSVWEGEQEWGDE"
     gene            5409..5720
                     /locus_tag="P5179_08080"
     CDS             5409..5720
                     /locus_tag="P5179_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRS95214.1"
                     /translation="MKRGEVRWYKFPQPNKKRPVLILTRNSVIDLLDEVTVAPLTTTI
                     RDIPSEVVLTPVDGVPTECAVNCDHLQTVSKAKIGALITSVSDIKMEEVSEAVAFALA
                     L"
     gene            complement(6232..6924)
                     /locus_tag="P5179_08085"
     CDS             complement(6232..6924)
                     /locus_tag="P5179_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95215.1"
                     /translation="MRYALVALCALAASPVLAQTQGDSLVAKFYSSVSVRNPAVTQLI
                     VPRQDGTKDTTGVPFDYEVAAGVSGNYGTIYFVMDYQYERQNAQHFVNQEYSLRQKKT
                     SYEVGMRLRDAEEGNLYILSGYVEGKLFGWVGVGVTRQHLRAWLNDGATLARFSLARN
                     NFHALKMDLVVNAEYETNPTQSRIFFSSAITNFKFGRFAVVPFYRHERVKPPGKPAKS
                     SYNGRVRLTIDI"
     gene            7266..7517
                     /locus_tag="P5179_08090"
     CDS             7266..7517
                     /locus_tag="P5179_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRS95216.1"
                     /translation="MAGLFSRLSLWFRQWFTTSEKRPEVGRNDLCWCGSGKKYKHCHY
                     EIDLRRGTLSRNTTPGAQREMMERVSKRMEKARARAKKR"
BASE COUNT         1491 a         2401 c         2307 g         1323 t
ORIGIN      
        1 accgtgttgg cgggcgcatt acagccatct cgcgcgggtc tccgagtgac gcctgcgtgg
       61 tccctgagcc tgtcgaaggg cagcaggcgt tgtatcgagg agcccgcgcg gcgcggaaga
      121 acctcgcgcc ccccgcctag tcccagtcct tccagtggtt gtccacgaac aggcagccgg
      181 ggtgctgtgc gtactgctca tccgcctgcc cagaaggatt gaccacgctc gtttaaacgg
      241 ttaccatgaa cgtgacgctc tccgttcgag tatgcttggc aagggaacgc ggaatggtct
      301 cgaaaccgga aatacaggcg ttggcagaca ggattgggaa agcgttccaa ccggagcgcg
      361 tcatcctgtt cggttcgcac gcgtatggcg tgccgggacc gcattctgac gtggatctgc
      421 tggttctctt gccatttgag ggccgcaatt tgagcaagtc ccttgaaatt ctgaatgccg
      481 tgaatccgcc gttcagcgtt gacctgctcg caaggcggcc ggacgacacc cggcgccggt
      541 acgaacaggg tgacccgctg attcgcgagg cgttggatcg aggcgaggta ttgtatgaac
      601 gaagtcgtca gggaatgggt gacaaaagcc gagggcgatt tccgcaccgc aagccgcgag
      661 tgccgcgcgc ggcaaaaccc taacttcgat gcagtttgtt tccacgcgca gcaatgcgtc
      721 gagaagttaa tgaaagcggc tctgatttcg gctgggttgg tgccaccaaa gacccacgat
      781 ctcgaacaac tcgatcgcct tctgaaacag acacacacca gatgggacgt cgatctgacc
      841 gatctgcgtt atctggcaag ggcttcagtg gcgtttcgtt accccggtga atcggcacag
      901 acaagaagga agctcaagaa gccctcgcca tctgctcgcg cctgagagac cagttgaggc
      961 tgcttctggt cccctccccc taatcccaat ccttccagtg gttatccacg aacaggcagc
     1021 cggggttgtc cctgatcaca ctgttttcgt catggccgcc caggtccacg agcagctcgg
     1081 tcatcgcgcc gtagtcgagc gtgttgtcct tgatcacgct gttcacaagc cccttgacca
     1141 cgatgccgta attcggcgaa aactccccgc cggagtcgcc gtcgttgcgc cagacgcgca
     1201 tggcgttgcc cgtgcagacg agcccgcgca cgccctcgaa gcgcagatgc gtgctctcca
     1261 gatcctccag cttccgccac atgggcgcgc cgctgcggaa gatcacgttg ccggtgatcg
     1321 tgatgacgtg cgccgggcgg ccgccgcgcg gcatcagggt aatgccggcc ccgccggacc
     1381 ggtcaatgta gttccccgtg atgttgtagt ggctgccgcc gtggatcacg atcccgccgc
     1441 cggcgttcca ctcgatgcgg ttgccggtca tggtcatgga ggcgttgtct tcgcgcgccg
     1501 cataccccgc cccgccgttg ccggaaaacc agttatccat gatgaagccg tcccacccgc
     1561 gcacggagag cccatccccc ttattgtggc tgaacatgca actgcggatg ctgaaacacc
     1621 agatatgggt gagccgcaca ccgtcgccgg tgaagtggtt cacgcggcag cgttcgatgc
     1681 gcggggtatc ctcctggtcg ccggagtcct ttttgtccac gaagatgccg tgaatgccgc
     1741 cgtcagagcc ggcggcagcg aggctcgcac cgtcgaggca gagcccgttc agcgtggcgc
     1801 cgattgcgcc ggtgaggtcc accaggcatt tcgccgaggg atcgccgagc cgtatgatgg
     1861 acccgccatc gtgccggaag ctccacgtcg ggttgccgat gagccccacg tgcggcggta
     1921 tcttgatggt ggaggaaagg aagacgccgt cgggaacgaa cacgctggcg cgcgtcccgg
     1981 cggctttgtc cagcgccttc tggatggccg ccgtgtcgtc gtgccggccg tcgccgatgg
     2041 cgccgaaatc ccggatgttg aacgatgcga agtcggacat gagagtgctc cgttgcgggt
     2101 ggatgggtga ggttcgcgtg ttctgaaaag tgggcacaaa accgctgcgt ggcaacaagt
     2161 tggccatggt catgggcttg cacccggcgc ggtggggagc ctgtccggcg gcgttatatt
     2221 tctgtgagag gatcttctga tcacccgcca acccgcagga cagcatgcct gacgacgacc
     2281 tcaaagagcg gattcgcgcc gccgtggaca tcgtgacggt gatcggcgag cggattcccc
     2341 tgaaaaaggc ggggcgcaat ttcgttgctc gttgcccgtt ccatcaggaa aagacgccgt
     2401 cgttcaacgt gaacccggag cgccagatct accactgctt tggctgcggg gccggtgggg
     2461 acgtgttcag cttcctcatg gagcacgaca aggtcagctt cccggaagcc ctgcgcaccc
     2521 tcgccgcgaa ggccggaatc gcgctccccg acaggcagag cgcccgtgcg cgcacgaatg
     2581 aacaggcgcg ggacatctac taccgggcaa atgccctcgc gcgggagtat ttcatcgccg
     2641 cgctgaacga ccccgccaaa ggcgaagccg cccgccggta tcttgaacgg cgtgggctca
     2701 cgaaggatac catcgccacg ttcggccttg gctttgcccc cgatgcgtgg gatggattca
     2761 tggcctatgc gcggccaaag gggctttcct acgaggttct cgccaatgcc gggcttcttg
     2821 ttcagaacga acagggccgc ttctacgacc ggtttcgcgg gcgcgtcatg tttcccgtcg
     2881 tcaacgacag cggccgcacg gtggccttcg gcgcgcgcac gctggacccg gccggcgagc
     2941 ccaaatacct gaactcctcg gaaagccccg tctaccagaa aggccgcatc ctctacggcc
     3001 tgtaccacgc gcgggacgcc atccggcagg agaactccgt cctcatcgtc gaaggctata
     3061 tggacgtcct ccagctcatg caggcgggca tcaggaatgt ggttgccacg tgcgggaccg
     3121 cgctcacgcg ggaacacggc gccatcctcc gccggtacgc cgacaggatc attctcctgt
     3181 tcgacggtga cgaagccggc atccgggccg cctcacgcgc gggtgacgtg ctgctggaag
     3241 ccggcatcga agggcacgtg gcgcttctgc ccggcgggga ggatccggac accctcgttt
     3301 ccgccgaagg gccggaggcg ctgcgctcca tcgccgagcg cggcatgccg tatctgcagt
     3361 tccggtggga aaccctcgcc cggcagaaca atctcgccac catctccggg cggaaccgcg
     3421 ccatcggcga catgctggac gtgatcgccc gcgtggacga cgagatgctg cgcggcatga
     3481 tggccaacca gatagcgcag tggggcgtaa cagacgaaac actggttcaa cgcgccatcg
     3541 cccggcgccg caggcagacc gtccgccgcg aacaggaagc ggctcccccg cagacgaagc
     3601 gctgggcgcc accccaccgc gaaaagcagc ttgtcgcgtg gatgatggac cggccggggg
     3661 tctgccagca ggtggcggaa atgggcgtgg agcgcttcag cgacccgctg ctgcaggaaa
     3721 tcgccgcgtc gctcatccgc ctgaaggatc tcggcgaact gccgtcagcc gcggcgctcc
     3781 ttgacgaaaa acgcgatgat tcccggtggg ccggcgcggt tgccgaactt gcccagatgc
     3841 actgcgacga gcgggaaatc ggaagggccg taacccagct tgtcgcgtcg ctccagttgc
     3901 ctcacctgaa acggcagata gacaccctgc gggcacgtct gcgggtcgag aagggcatcc
     3961 gggaaaccat cgaaatcacc acggagattc agcgcctcgg tcgggagatg gaaattctca
     4021 acgacctcct ccacagcggg gagaactgag ccgcaccccg caccgccagc gccagagaca
     4081 ttgacactgc ggcttctgtg cgtacgttta cagctaccag taccttctca ggaaactgtg
     4141 tcggaacacg caggcgcggg accgagagcg tgaagaaaca aacacaacac accgtcagcc
     4201 ctgtccgtaa ggggctcgcg aacgagcagg tgatgagtgc catgaaccag gatcgtgaag
     4261 gcgacctgct caccggcgaa caggtgtttg cggtgctcag ccgccacaag aacgcgctgg
     4321 cagagcgata cggagtcaac tcgctgggtg tgttcggttc gatggcgcgc ggagactccg
     4381 gcccctccag cgatgtagac atcgtgttca cgaccgattc gccgaacctg tttcgcagcg
     4441 tccgcatgaa actggagctg gagcggctgc tccggaggcc ggtggacatc gtgcggctac
     4501 gcgacaacat gaacccccga ctgaaggctc gcatactccg cgaggcccgg tatgtctgac
     4561 tctgacgtgc tgaaagcgcg cctcgaggcc gtcctcgaag cactgcaacg gattccccgc
     4621 cggttcgcgg ccatccgggc accggaggat ttcgaaaaga cggaggcagg acgggacaat
     4681 ctggacagca tttgcatggt cctcgtcgcc tcgggagagg cattcaaaca gatcgatcga
     4741 aaaacaaacg gccggttcct gaggaactac ccggaaatcg agtgggaagg tgttatgggt
     4801 gtgcgggacg tcattgcgca cggatacttt gacgttgacc cggatcaggt gttcgacatc
     4861 tgcaaaaacg acattcccac actcatcggc acggtggaaa gaatgatcgc cgatttgcgg
     4921 tgatctgtcg tcatccgagc gatcgtcgcc agccgcgagc cccctaccgt catcctgagc
     4981 gccgtccgcc gttgtggacg gttttcacat ccgacaagcc ggacgcatcg aggaaacccc
     5041 cgctgccatc actcccgttg gctacgcccg gccattgcca accggaagca acccgcataa
     5101 gtcccgcaac acatattgac agcctggcta tcatgtgtat gtttatgact ataatcgcca
     5161 acacgcagga gtcaggctat gagaactgtt caactcactc tggatgaaaa cctcgtacgc
     5221 gacgtggatc gacttgtgaa gcagcgcaac accacacgat ccgcgttcac gcgggacgct
     5281 ctgcaggaag caatcaaggc agcacgcacc cgcgagatgg aagagaaaca ccgggccggg
     5341 taccggaaac tgccggtcag gaaaggcgaa ttctcggtct gggaagggga acaggaatgg
     5401 ggggatgaat gaaacgaggt gaggtgcgct ggtacaaatt cccccaaccc aacaaaaagc
     5461 gaccggtact catcctgacg cgcaattcgg taatcgacct tctggatgaa gtgaccgttg
     5521 cacccctcac caccaccatt cgcgacattc caagcgaggt ggttctgacg ccggttgacg
     5581 gcgtccccac ggagtgcgcc gtcaactgcg accatctcca gacggtgtca aaggcgaaaa
     5641 tcggcgcttt gatcacctcc gtgagcgaca tcaaaatgga ggaagtgagc gaggctgtcg
     5701 cgtttgcgct tgcgctctga ccgtccctct ccgcctgcct ctgagtggcg ggacggccag
     5761 aggaacgggg gcggtggtcg cgcggccaac cggcgttgct cagccccgga tcgtcatcct
     5821 gggcgccgtc cgccgttgtg gacggttttc acatccgcca gcgcaaacgc atcaaggata
     5881 cccccactgc caacgctgcc caccgcagtc tatcacagcc gggcgacgcg taccgaggcc
     5941 cctcgtgtcc cccatgccga atcgcccatg ccgccgtcat tcctgctcct cttttgccgt
     6001 cattcccacg gaggcgggaa tccacgccat gccgcttcgg gcgaaaaggg agcctcgtac
     6061 gcacgcctcc tcacggaatc tggaatcgcg gcaccatgta cccgtgtttt ttctggagtt
     6121 ccgcctccgc gggaaggacg ggttactctg cggcaggtaa cgccaaggtg cacggcagag
     6181 cagaatctcc cgcagcgcac accgcccgac ggactgattc gcgcgcgccg ctcaaatgtc
     6241 gatcgtcagc ctcacacgcc cgttgtagga gctcttcgcc ggcttccccg gcggcttcac
     6301 gcgttcgtgc cggtagaacg gcaccacggc gaaccggccg aatttgaagt tcgtaatggc
     6361 actcgagaag aaaatgcggc tctgcgtggg attcgtctcg tattccgcgt tgaccaccag
     6421 atccatcttc agtgcgtgga agttgttccg cgccagcgag aaccgcgcaa gcgtcgcccc
     6481 gtcgttcaac cacgcccgca gatgctgccg cgtcaccccc acgccgaccc agccgaacag
     6541 cttgccctcc acgtagccgc tgaggatgta gaggttcccc tcctccgcgt cgcgcagacg
     6601 catgcccacc tcgtacgacg tcttcttctg acggaggctg tactcctggt tgacgaaatg
     6661 ctgcgcgttc tgccgctcgt actggtaatc catcacgaag tagatggtgc cgtagttccc
     6721 cgaaacacct gccgccacct cgtaatcaaa cggcacgccg gtggtgtcct tcgtgccgtc
     6781 ctgccgcggg acgatcagtt gcgtgacggc gggattgcgc acggacaccg acgaatagaa
     6841 cttggcgaca aggctatccc cttgcgtctg cgccaaaacc ggcgacgctg caagcgcaca
     6901 cagggcaacc agggcatatc tcatggcagg ctccagaagg gaatcacgcg gtctggcgcg
     6961 agggtgaaac cggatctacc gccgtaccgc gttgtccgca cccacagggt ccctcgccga
     7021 gtgctccgaa acagccactg acgcaggcgc ggaacgcggt gaagaagtcg aaaatgccgg
     7081 cacggcaagt caaggaaagt gtaggcaatc ggcgcgcccg tgtcaatcgc ggttccgggt
     7141 tgccgcgtcc gggctgcccg cagtgtggag gcaccgggac accgcgggcc tacccgctga
     7201 tggcgggcgg tcgtacattt ctttcttgat accccaccat ctcgaacacg aaagaatgca
     7261 ctgccatggc cggtttgttc tcccgcttgt cgctctggtt ccgccagtgg ttcaccacct
     7321 ccgaaaaacg cccggaagtc ggccgcaacg acctctgctg gtgcggaagc gggaaaaagt
     7381 acaaacactg ccattatgaa atcgacctcc ggcgcggcac gctttcccgc aataccaccc
     7441 ccggcgccca gcgcgaaatg atggaacgcg tcagcaagcg catggaaaag gcccgcgccc
     7501 gcgccaaaaa acggtaatcc cg
//
LOCUS       DAOTNG010000144         7428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8751_length_7428_cov_4.528821, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000144 DAOTNG010000000
VERSION     DAOTNG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8751_length_7428_cov_4.528821"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="P5179_08095"
     CDS             complement(<1..643)
                     /locus_tag="P5179_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95217.1"
                     /translation="MAARHQFLLFAALVFLCAPLRRATGVSGTPVSGEIRTTTWTKAG
                     SPYRVTNTIRVPQGQLLTIEPGVDVLFDDDVPFVVEGRIHAVGMQTDSIRFLPGTTEW
                     GGLRISGGDTSTLAYVRISGGNANGNPDSLWNRGGGLFVNVARVGVSHSVISGNSAAM
                     RGGGLYITDPASHVTLANCRVTDNATSPGSSNHGGGAYIYGSTVLLKHTLIAGN"
     gene            complement(821..2281)
                     /locus_tag="P5179_08100"
     CDS             complement(821..2281)
                     /locus_tag="P5179_08100"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HRS95218.1"
                     /translation="MDPTGFLCVAPSCVGVGESFPLKVKVLGPVRPIACSGNWNDPKP
                     ALRGPFNLNVDRKIQYHDNCLPEWRGALKVDGEGRLTGSRDLVFDGVHQGTFPGDSRP
                     IRVFDGFSFAEPGIHFIRLVDEKSGVEGWSNPVNVTPEPPRSRIYWGDPHWQTFFSDG
                     IRCPEELYAFARDEGFLDFGAITDHMEGVTDRQWDYFQAVTNDYNRPGRFVTLIGQEW
                     TNHNPAAGAPGHRNIYYRQSAGPVLRSNASDCDTLEKLWKKLDSISRVDSIAIPHHSA
                     NVIMGVDWEQGWNPKYEKAVEIHSVWGNSERSREDGNPMAIQHLNGEIRGRHVVDALR
                     KGFRLGFVGGGDIHDGRPGDALHQESYPPFPTRFWPEGFTAVTVPVLTREAVFDAMRD
                     HATYATTQSRIYLDVRAKPTRNGLTVAVTAASEEGVVEASIVSNGGDVGALRPDDNPR
                     VLRDRKTTVHVDPGGFCYVRIITDRGNMAWSSPVWV"
     gene            complement(2292..3644)
                     /locus_tag="P5179_08105"
     CDS             complement(2292..3644)
                     /locus_tag="P5179_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS95219.1"
                     /translation="MLTITESARTIPIVHEADICVIGGSCTGVFAAVRAARLGASVAV
                     VELGGFFGGVATAGLVNVWHSLKDVHDERQIIGGLTQETIDRLHRRAAVEFGTSPHAA
                     YFLNTEELKIELDNLITEANVRPFLHARFVAPVVADGRLDAVIVEDKSGRRAIRAKYF
                     VDASGDGDLIHRMGLPTRFLGDIQPPTACCIIEGLGKVEEANPGFKLNEAVFNPEFPE
                     ALKLGFLWAAWVAGSRDMKMVAGTRVANADCSDADQLTAAEMEGRRQIRAMVDILRNH
                     FARGEEVFLRGVPSYIGIRETRHAQCLHSVTEMELLTGHAFEDTIGCGTYHVDVHHSE
                     KPGLTFRNLDGTEEWVVPGRPPVRGRWRPEGEDTPAFYQFPYRSLVPEGSKNVLVAGR
                     LIDCDRGAYGALRVMVNCNQTGEAAGVASTLALRENGAVADVNSAKLRNAMREGGSLL
                     "
     gene            complement(3782..5815)
                     /locus_tag="P5179_08110"
     CDS             complement(3782..5815)
                     /locus_tag="P5179_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS95220.1"
                     /translation="MPAPQSHLDLYVSPLGRDNWSGRLAEANADDTDGPFATVTKARD
                     TIRELKRATRLPASVTVWLRGGRYILKAPLTFTHADSAPVTYAAFPGETPVLDGGTRI
                     TGWQKESLNGKEVWVTTIPEVAEGKWYFRQLFVNGARRSRPRLPKEGFYRVADYERPH
                     AEGPWGDIFMGADAFQCAPGHVQSWKNITDIDIVVIHWWVEERVSMAGFDPATNTVKL
                     ANKTGFTLKDDVADGLARYYVENVEEALSDPGEWYLDRSTGKLTYVPMPGEDIETAQV
                     FVPRLRKLVQVTGDADAENYVEFLRFEGLTFEHAEWDYCLSNQAAHKTPAVITFEAAR
                     SCAFENCTVRHIGGYAVELRDGCQNIRVVGNTLVDLGAGGVHLNGADALGPRARRTGN
                     NRITDNVIGEGGRVFLAAVGVLSRHSFGNHISHNHIYDLYYSGVSCGWVWGYHENVSQ
                     NNRIEKNHIHDIGHGILSDMGGIYTLGVQPGTMLRGNLIHDIEKSNYGGWAIYMDEGS
                     SHIISENNICYNTSSQGFHQHYGRENVVRNNIFAFGREGQATVSRYEDHLSVTFDRNI
                     FIGDNQPIFAMGRPEQRIFLSNNNLFWDVARAPWCGASTRNEKGEWGYKQEYSFEEWR
                     KLGYDPQSIVADPKCRDFAARDFTLEPASPAFALGFEPIDMSDVGPRPPEKRE"
     gene            complement(5867..>7428)
                     /locus_tag="P5179_08115"
     CDS             complement(5867..>7428)
                     /locus_tag="P5179_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95221.1"
                     /translation="KENALVKSVIHSNRPPFRLASNVIYFHDWRYVDHGSHQWLTETG
                     KGVPLFTTESLPPLRYRCVDMPYGIRLVAQKAEKGQPVITPEMARAVFLFGGTLIHDE
                     GVYRFWYECWPEEGIGTPHMGVRNHVRYAESDNGVDWRFPNAGVVNYRGDTRNNIVYG
                     AMLRDDDVGYHGGSVFKDPSAPPEERYKGFHLGHIPDDMYADYLRERPDAVDPYHRNQ
                     GLFGAVSPDGIHWKPIREPLVAHYSDTHNCCTYDVQQGKYVAYCRNWFFRRRTIGRME
                     TDDFRRFPLPEDLFWPGATMAPHDLWYSQPKTLMPGTIDYHVMFPMRWSLATDSFHFH
                     LATSPDGVVWGFVPDASGYTPGEGVCSPGCAGEWDGGVVTPGIGLVDLPGNRTGMLFA
                     GSPVPHKHPRKPPLGKIAWATWKKERLVALECPMEGFFATQPLVFEGRTLRLNAKTQF
                     TGFVEVEACKPDGSPIPGRSFADCDRFNGDETDHPITWRGESDIGHAENAEVRFRVRM
                     RSADLFAMRFV"
BASE COUNT         1399 a         2337 c         2191 g         1501 t
ORIGIN      
        1 tgttccctgc aatcagcgtg tgcttcagca gcaccgtgct gccgtagatg taggctccgc
       61 cgccgtggtt gctgctgccg ggagaggttg cgttgtccgt cacccggcaa ttggcaagtg
      121 tgacgtgact tgcaggatca gtgatgtaca ggcctccacc gcgcatcgcc gcagagttgc
      181 cggaaattac cgagtgcgaa acacccactc tggccacgtt tacaaaaagc ccacctccgc
      241 ggttccagag cgaatcgggg ttgccattcg cgttcccccc gctgatacgc acatacgcca
      301 gggtgctggt atcgccgccg gagatgcgca agccacccca ttccgtcgtg ccggggagga
      361 aacggatgct gtccgtctgc atccccaccg cgtgaatgcg cccttcaaca acgaacggaa
      421 cgtcatcgtc gaacagcacg tccacgcccg gttctatggt gagaagctgg ccctggggga
      481 cgcgaattgt gttcgtgacg cggtacgggc ttcccgcctt cgtccaggtg gtggtccgga
      541 tttcacccga aaccggggtt ccggaaacac cggttgcccg gcgcaaaggc gcacacagga
      601 ataccagcgc ggcaaaaaga agaaattgat gtctcgcggc catacatctc ctcctcatcc
      661 tcctcacgcg tgcagaggcg ctgcacgcga gcccgacgag atccttacgc atgcaaaaga
      721 atgatgcaca gtgaatggca agaatgtagt cacccacccg tccgtatcag agcagggcaa
      781 ccgcaggacc gggcaacgcg cggtgcctga gcgttcgtac tcacacccag acggggctgg
      841 accacgccat gttgccccga tcggtgatga tcctgacata acagaaacca cccggatcga
      901 catgcacggt cgttttccgg tcccgcagca ctcgcgggtt gtcgtccggt cgcagcgcgc
      961 caacatcgcc cccgttcgag acgatggagg cttccacgac gccttcctct gatgccgcag
     1021 tcaccgcaac ggtcaggccg ttcctggtcg gcttcgctct cacatcgagg tatatcctgc
     1081 tctgggtcgt tgcgtacgtg gcgtgatccc gcatcgcgtc gaaaaccgct tcccgtgtca
     1141 gaaccggcac ggtgacggct gtgaaccctt ccggccagaa ccgcgtcgga aacggaggat
     1201 aactttcctg gtgcaacgca tctccggggc gtccgtcgtg gatatcgccc ccgcccacga
     1261 aaccgaggcg aaatcctttc cgcaacgcgt caaccacatg ccggcccctt atttcgccgt
     1321 taaggtgctg gatggccatg gggttgccgt cctcgcggga acgctcactg ttgccccaca
     1381 ccgagtgaat ctcgaccgct ttttcatact tcgggttcca accctgttcc cagtcgacac
     1441 ccatgataac gttcgcgctg tggtggggaa tcgcgatgga atcaaccctc gaaatggaat
     1501 ccagcttttt ccacagcttt tcgagcgtgt cacaatctga agcgttgctc cgcaggaccg
     1561 gtccggcact ctggcggtag tagatattcc tgtgccccgg cgctcctgcc gcaggattgt
     1621 ggttcgtcca ctcctggcct atcagcgtga caaaccgccc cggccggttg taatcattcg
     1681 ttacggcctg gaagtaatcc cactggcggt cggtaacccc ctccatgtgg tccgtgatgg
     1741 cgccgaaatc gaggaaccct tcgtcccggg cgaatgcgta cagctcttcc gggcagcgga
     1801 tgccatcgct gaagaacgtc tgccagtggg gatcgcccca gtagatgcgc gaacggggcg
     1861 gttccggggt gacgttgaca gggttcgacc agccctcgac gccagatttt tcgtccacaa
     1921 gccgtatgaa atggatgccc ggctccgcga aggaaaaccc atcgaaaacc ctgatgggcc
     1981 gcgaatcgcc gggaaatgtt ccctggtgga caccgtcgaa cacgagatca cggctccccg
     2041 tcagcctgcc ttccccgtcc actttcagcg caccccgcca ctcggggagg caattgtcgt
     2101 gatactgaat cttgcgatcg acgttaagat tgaacggccc acgcaacgcg ggcttcggat
     2161 cgttccagtt gcctgagcag gcgatggggc gaacaggtcc aagaaccttg actttgaggg
     2221 gaaatgattc gccgaccccg acacaggaag gcgcaacgca caaaaaacct gtcggatcca
     2281 cacacacccc tttagagcag agacccaccc tcgcgcatcg cattccgcag tttcgcggag
     2341 ttgacatccg cgactgcgcc gttttcccgt agcgccagcg tcgaggccac ccccgctgcc
     2401 tcccccgtct ggttgcagtt gaccatgaca cgcagcgcac cgtacgcgcc gcggtcgcaa
     2461 tcaatcaggc gccccgccac cagcacgttt ttcgagccct cgggcacaag cgaacggtac
     2521 gggaactggt agaacgccgg cgtatcctcg ccttcaggtc gccaacgccc tcgtaccggc
     2581 ggtcttcccg gaaccaccca ttcctccgtc ccgtcgaggt ttcggaaggt gagccccggt
     2641 ttttcgctgt ggtggacgtc gacgtgatat gtaccgcacc cgatcgtgtc ttcaaacgca
     2701 tgcccggtga ggagttccat ttcggtcaca ctgtggaggc actgtgcgtg gcgcgtttcc
     2761 ctgataccga tgtacgacgg caccccgcga aggaatacct cctcgccgcg cgcaaaatgg
     2821 ttccggagga tgtccaccat cgcgcggatt tgccgccttc cttccatttc ggcggcggtg
     2881 agctggtctg cgtcggagca atcggcattc gcaacccggg tgcctgcaac catcttcatg
     2941 tcgcgagatc ccgccaccca cgctgcccag aggaacccca gctttaacgc ttccgggaac
     3001 tccgggttga acaccgcctc gttgagtttg aaccccggat tcgcttcttc aactttccca
     3061 agcccctcga tgatgcaaca cgcagtgggg ggctggatat cgcccagaaa acgcgttggc
     3121 aggcccatcc ggtggatcag gtcgccgtcg ccgctcgcat ccacgaagta ctttgcacgg
     3181 atcgctcgac ggcctgactt gtcctccaca atgacggcgt ccagccggcc atccgcaacc
     3241 actggagcga caaaccgggc atggagaaac gggcggacgt tcgcctcggt gatcaggtta
     3301 tcgagctcga ttttcagttc ttccgtattc aggaagtagg cagcgtgggg agatgtgccg
     3361 aattccaccg cggcgcggcg gtgcagccgg tcgatcgtct cctgcgtaag gccgccgatg
     3421 atctggcgct cgtcgtggac gtccttgagg gagtgccaca cgttcaccag tccggccgtc
     3481 gcgacgccac cgaaaaatcc accgagttcg acgacggcaa ctgaggcacc gaggcgggca
     3541 gccctgactg ccgcgaatac cccggtgcaa ctgccgccga tgacgcagat atcagcctcg
     3601 tggacgatgg gaatggtgcg agcggattca gtgatggtga gcatgtgttg ctcctcgcgc
     3661 atttctgttg gcggctgtct ccgctagaca gccctgccac gggtaaccac cctaagaaat
     3721 cggtcccata cgacacatac gtcctatgcc gaaccgccaa cagggcatat tgcttccggc
     3781 gctactcccg cttttccggc gggcgcgggc ccacgtccga catgtcgatc ggctcgaacc
     3841 cgagcgcgaa ggccggggaa gccggttcca aggtgaaatc tcgcgccgcg aagtcccgac
     3901 atttcggatc cgccacaatt gactgtgggt cgtacccgag tttccgccat tcttcgaatg
     3961 aatactcctg cttgtacccc cactcgcctt tctcatttcg cgtgctggcg ccgcaccacg
     4021 gggcgcgggc gacatcccag aacaggttgt tgttggaaag gaaaatacgc tgttcaggcc
     4081 ttcccatggc gaaaatcggc tggttatccc cgatgaagat gttccggtcg aacgtcaccg
     4141 acaggtgatc ctcataacgg gagaccgtcg cctgcccttc gcggccgaag gcgaagatgt
     4201 tgttgcggac aacgttttct cgcccgtaat gctgatggaa cccctggctg gaggtattgt
     4261 agcagatgtt gttctcgctg atgatgtgcg agctgccttc gtccatgtaa atcgcccagc
     4321 caccgtaatt cgacttctca atgtcgtgga taaggttgcc ccgcagcatg gtgccgggct
     4381 ggacgccgag cgtgtagatg ccgcccatgt cgctcagaat accgtggccg atgtcgtgaa
     4441 tgtggttctt ctcgatgcgg ttgttctgtg acacgttttc gtgatatccc cacacccaac
     4501 cgcacgacac gccgctgtag tagagatcgt agatgtggtt gtgggagata tggttgccga
     4561 atgaatgccg ggaaagcacc ccgaccgctg cgagaaacac gcgcccgcct tctcctatta
     4621 cattgtcggt gatgcggttg ttaccggtcc gccgtgcgcg tggaccgagc gcatccgctc
     4681 cgttgagatg gacgccgcca gcgccgaggt caaccagggt gttacccacc acgcggatgt
     4741 tctggcagcc atcccgcagt tcgacggcat agccgccgat gtgtcgcacc gtgcagttct
     4801 cgaaagcgca cgaacgcgcg gcctcaaagg tgatcaccgc cggtgtttta tgagcggcct
     4861 gattcgacag gcagtaatcc cactccgcat gctcgaaggt tagcccttcg aagcgaagaa
     4921 actcaacgta gttctccgcg tcggcatccc cggtcacctg cacgagcttt cgaaggcgcg
     4981 gcacgaacac ctgtgcggtt tcgatgtcct ctcccggcat cggcacgtaa gtcagtttcc
     5041 cggtggacct gtcgaggtac cactcacccg ggtcgctcag cgcttcctcc acgttttcga
     5101 cgtagtaccg ggcaagaccg tcggcgacgt cgtctttcag cgtgaatccc gtcttgtttg
     5161 cgagtttgac cgtgttcgtg gcggggtcga aacctgccat ggagactcgc tcttccaccc
     5221 accagtggat caccacgatg tcgatgtctg tgatgttctt ccagctctgg acatgtcccg
     5281 gcgcgcactg gaaggcatct gctcccatga agatatcccc ccatggtcct tcggcgtgcg
     5341 gccgttcata gtcggcaacc cggtagaagc cctccttcgg caacctcggg cggctgcgtc
     5401 ttgcgccatt cacaaacaac tggcggaaat accatttccc ttccgctacc tcggggatgg
     5461 tagttaccca gacttccttt ccgttcagcg attccttctg ccatccggtg atccgcgtcc
     5521 ctccgtcaag cacgggtgtt tcgcccggga acgcggcgta ggtgacgggc gcggaatcgg
     5581 cgtgcgtgaa agtcagcggc gctttgagga tgtaccgccc tccccgcagc cagaccgtta
     5641 cggatgcggg cagtctggtg gcacgtttca gctcgcgaat cgtgtcgcgt gccttggtca
     5701 cggttgcaaa cgggccgtca gtgtcgtcgg cgttggcttc cgcgaggcgt ccggaccagt
     5761 tatcgcgccc aagcggggat acgtagagat cgagatggga ttgcggcgcg ggcatggaaa
     5821 ctcctcctgt atgcagatgg atacgttcaa tcgcgcgagc gcggtgttac acgaatctca
     5881 tggcgaacag gtcggcactg cgcatccgga cgcggaaacg aacttcggcg ttttccgcat
     5941 gcccgatgtc gctttcgccg cgccacgtga tgggatggtc cgtttcatcc ccgttgaagc
     6001 ggtcgcaatc ggcgaagctg cgcccgggaa ttggtgaacc gtcgggcttg catgcttcca
     6061 cctcaacgaa accggtgaat tgcgttttcg cgttcagccg cagcgtgcgt ccctcaaaaa
     6121 caagcggttg tgtagcgaag aaaccctcca tcgggcattc cagcgcaaca agacgctctt
     6181 ttttccatgt cgcccacgcg attttgccca gaggcggctt tcgcgggtgc ttgtgcggca
     6241 ccggtgagcc cgcgaacagc atcccggtgc ggttcccggg aaggtccacg aggccgattc
     6301 cgggggtgac gacgcccccg tcccactcgc ccgcgcaccc cggtgagcag accccctcgc
     6361 cgggcgtgta gccgctcgcg tccggaacga aaccccagac gacgccatcg gggctcgtgg
     6421 cgagatggaa atggaacgaa tccgtggcaa gcgaccaccg catggggaac atcacgtggt
     6481 agtcgatggt gcccggcatc agcgttttgg gctggctgta ccagagatcg tggggagcca
     6541 tcgtggcacc gggccagaag aggtcctctg gaagagggaa acgtcgaaaa tcgtccgttt
     6601 ccatccgccc gatcgtgcgg cggcggaaga accagttgcg gcagtaggcg acgtatttcc
     6661 cctgctgaac gtcgtacgtg cagcagttgt gcgtatcgct gtagtgggcc acgagcggtt
     6721 cgcggatcgg tttccagtgg atgccgtccg gcgacacggc accaaaaagg ccctggtttc
     6781 tgtggtacgg gtccacggca tccggccgtt cgcggaggta gtccgcatac atgtcatccg
     6841 ggatgtgccc cagatggaac cccttgtacc gttcttccgg aggggctgag gggtccttga
     6901 acacgcttcc gccgtgatac cccacgtcgt catccctgag catagcaccg tagacgatat
     6961 tgttgcgggt gtctccccgg taattgacca ctccagcgtt gggaaagcgc cagtctacgc
     7021 cgttgtcgct ttcggcatag cggacgtggt tgcggacacc catgtgcggt gtgccaatgc
     7081 cctcttcggg ccagcactcg taccagaacc ggtagacgcc ttcgtcatgg atcagtgtgc
     7141 caccgaacag gaacactgca cgtgccatct cgggcgtgat gacgggttgt cctttttctg
     7201 ccttctgcgc cacaaggcgg atgccatacg gcatgtctac gcaacggtaa cggaggggag
     7261 gcaagctctc cgtggtgaaa agcggcacgc ctttaccggt ttcagtgagc cactgatggg
     7321 agccgtgatc cacgtagcgc caatcgtgga agtagataac gttggacgcg aggcgaaacg
     7381 ggggacggtt gctgtggatc acggacttca cgagggcgtt ctccttcg
//
LOCUS       DAOTNG010000145         7409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8774_length_7409_cov_4.877346, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000145 DAOTNG010000000
VERSION     DAOTNG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8774_length_7409_cov_4.877346"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1095)
                     /locus_tag="P5179_08120"
     CDS             complement(61..1095)
                     /locus_tag="P5179_08120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRS95222.1"
                     /translation="MLLAWSFDRVPSARVATFENGSRQGLASSTERKRDLALNLGAST
                     GVGHYVIVSVTAPREFLDVLTDCAAELGTLGSYESGDDPVNLTFYFRPPLHEEAARSA
                     FLESVPSHAASVVHVSFTTETNRDWEQEWRRNLHPLRVGRTWLVHTSWQSPAGYPTRI
                     PIVIDPKMAFGTGTHATTRLCLEELERFPLRGTRVLDVGTGTGILAIAAARSGAENVI
                     AVEIDHEAIACARENLSLNGIGNTVTLREGTIAAVPEHGFDLIVANIELRTLIAVGEA
                     LRARIHPDGRAVFSGILQQEADLFVQAMTKVGWQTMRRTRKFDPATGDGWVCCVATPS
                     FPAGAKSKLS"
     gene            1291..2994
                     /gene="argS"
                     /locus_tag="P5179_08125"
     CDS             1291..2994
                     /gene="argS"
                     /locus_tag="P5179_08125"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRS95223.1"
                     /translation="MTRVFAPLISIFAKATELEEREVAGLLCPPPSAELGDFAFPCFV
                     LAKRLRKSPAQIAAELAERVNSNDTIASAVAVGPYVNVRLHRAPFISTLLEEVRNQGE
                     QFGSSSSGNGKCVVLDFSSPNMAKPFHVGHLSSTIIGGSLSRLFAYQGYRVVRINHLG
                     DWGTPVGQQITAFKRWGDGTELDRHPIAHMVDLYQRFHREKAENPGLEEEAREWFRKL
                     ETGDKEAREFWEHIRSVSIQELNRAYARLNVEFDAYLGEAFYEGMLQETIEEVQKAGI
                     TEVSDGALIVPLDSKGIKTPLILRKSDGSTIYATRDIAAARYRAENYRFDLCLYVVAH
                     DQELHFRQLFATLELMGYDWAKRCVHVQFGHVHGMSTRKGGAILLDELLDRATEKVRE
                     TVMANQTLFRDVPAAEIDEVCEAVGVGAVIFGTLNHRRTKDVEFSWDNALNFNGETGP
                     YVQYAHARLCSILRKAGDEPRGAANVELLTHDAEWALVKELARFPEVVEEACAQYEPS
                     CVARYAVDLARAFTVFYDQCRVLGEEPELSAARLSLVNTTRVVISSALHLLCMKTPER
                     M"
     gene            2994..3704
                     /locus_tag="P5179_08130"
     CDS             2994..3704
                     /locus_tag="P5179_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRS95224.1"
                     /translation="MTMSTLTLPELPVYVCRRTARPISVDGILDEPDWGAAEPMRLVR
                     TESGAVPEQPTSVRVLWDDEYLYVAFECVDTDVWATIEERDGALWDEEVVEVFVDDDS
                     DSRTYLEYEINPLNALVDLYVLNRTGRRDAIKFMLDWNSSGIRHAVRVDGDPRRRGSS
                     DRGWTAEWAIPFGDFTTAPNIPPRNGDAWRINFYRIDRGDRDEYSAWSPTGAINFHVP
                     ERFGTLVFSTETVTRKDH"
     gene            3721..4389
                     /locus_tag="P5179_08135"
     CDS             3721..4389
                     /locus_tag="P5179_08135"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRS95225.1"
                     /translation="MNDTPLVLCRRTAEQITVDGVLSERSWAAVEPITLCRTDTGDAA
                     RFRTEVRLLWDDTYLYASFHCVDPDIWANMVLRDAPLWEEEVVEMFLDPNRSGNAYFE
                     FVVNPLNTLTDVFVLNRWGQFKPLRDWNAEGVKHAVVVDGDPSDPLSEDRSWTMECAI
                     PFRELVTAPNIPPKSGDSWRGNFYRIEQGYEWQEYTAWSPTGEVNFHHPERFGTIVFT
                     SETA"
     gene            4463..4996
                     /locus_tag="P5179_08140"
     CDS             4463..4996
                     /locus_tag="P5179_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95226.1"
                     /translation="MKRLWLFLILAPHVACAGGLAGLGFTANVPRQTLGGVIVYSWTY
                     ADVKVSRNFLGIWRDTYESISVPEAVYEFGDSLINSARTYKTVDAGLQARLSHRTTAY
                     LGLGVTETRHLYQFLDDFRILGRNGQYWVEHEVDYGINVNGGVYLRVAGPLYGQIGFN
                     TNPRGLDLGVAIIFASP"
     gene            5028..5486
                     /locus_tag="P5179_08145"
     CDS             5028..5486
                     /locus_tag="P5179_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95227.1"
                     /translation="MRGFCSHVVIAAAATAVFFSWLCGCGILSSTGTQNPLWGVWSGP
                     YPSSLGTQADSSRFAFTSKRVYLFFALKADGTILAREFGYYSSNGYLMILSSDPGGNL
                     DREEIFYEFVGESLVATIRGQRVVYRRVGGVEWADDLGSAAHLKEGAVGR"
     gene            5495..6055
                     /locus_tag="P5179_08150"
     CDS             5495..6055
                     /locus_tag="P5179_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRS95228.1"
                     /translation="MSRGGDKLSAAFQAWKSVLPRLEGAVAADFGSNVGGFVQCLLAQ
                     GITRVYAIDTGYGVLAWELRNDPRVRVMERTNALHVVLPEKVDLVTIDVAWTKQRLIL
                     PAAFRLLRDGGLVVTLVKPQYEATQKERRGGVLPGERVPDVLMRVRAEVRSVGGEILA
                     ELESPIKGAGGNTEFLWLVRPGNATF"
     gene            complement(6162..7031)
                     /locus_tag="P5179_08155"
     CDS             complement(6162..7031)
                     /locus_tag="P5179_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95229.1"
                     /translation="MYASIRDDTVQSAYPDIITGLKALGLSAIEANFTRQVSLPSLTD
                     KTVFFPVTTSAEIAEYKKHLDQYNVKISAFLLANNLNADDLEDELAWCTKALKVAHAL
                     GVKAVRIDSAMKGQQELPFDVRVEKFVKGIRALIDATPDAPVDMGIENHGYQGNDPNF
                     LHGVFDGVGSSRIGMTLDTGNFYWRGHALEKTYAVIKEFAPLAKHTHIKNINYPEDKR
                     NITREMGWEYGKYVCPIPDGDVDHTIVVGYLRDAGYDRDLCIEDESLGKFPIEERKNV
                     LLRDAEYIKGLAK"
     gene            complement(7154..>7409)
                     /locus_tag="P5179_08160"
     CDS             complement(7154..>7409)
                     /locus_tag="P5179_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95230.1"
                     /translation="LNPIGFCTATTFYHSAIWSGVFITWATKIIVHKIGGLSLYRRCY
                     PFFIGLVAGHVAGAIFCLAVDWIWFPGAGHDIRTMIYFFG"
BASE COUNT         1465 a         2032 c         2286 g         1626 t
ORIGIN      
        1 tcccgttcgg tttgcagggt tcgcaagttc gatacgtgcg cgccctgcgc cggagatctc
       61 tcaggaaagc tttgatttcg cacccgcggg gaacgacggc gtggcaacac agcagaccca
      121 cccgtcgccc gtcgcagggt caaatttccg cgtgcggcgc atcgtttgcc atcccacctt
      181 cgtcatcgcc tggacgaaca aatctgcttc ctgttggagg atcccgctga ataccgccct
      241 tccgtcggga tggattcggg cccgcaatgc ctctcccact gcgatgagcg tgcggagctc
      301 aatgttggcg acaatcaggt caaaaccgtg ttcgggaacc gcggcgattg ttccttccct
      361 aagcgttacg gtgttcccta tcccgttgag gctgaggttt tcccgagcgc acgcgatcgc
      421 ctcatggtcg atttccactg cgatcacatt ctctgcgccg gacctggcgg cggcgatggc
      481 gagaatgccg gttccggtgc ccacatcaag cacgcgcgta cccctcagag gaaaccgctc
      541 aagttcctcg aggcaaagcc gcgtcgttgc gtgagttccg gtaccaaacg ccatcttcgg
      601 atctatgacg atcggtatcc gggtcgggta tcctgctggc gattgccagg aggtgtgcac
      661 caaccatgtc ctccccaccc gcagaggatg aagattgcgc ctccactcct gttcccagtc
      721 gcggttcgtt tccgtcgtga acgatacatg caccacgctg gcggcgtggg acggtaccga
      781 ctcgagaaag gcggagcgag ccgcttcttc gtggagtggc ggacggaagt agaacgtgag
      841 attgaccggg tcgtccccgg attcatatga accgagagtt ccgagctctg cggcgcaatc
      901 cgtcaagaca tcaaggaact cacgcggcgc ggttaccgaa acgatgacgt aatgacccac
      961 tccagtcgat gcccccagat tcagtgccag atcgcgcttt cgttccgttg aggaagccaa
     1021 tccctgccgt gaaccgttct cgaatgtcgc gacccgtgcc gacgggaccc tgtcaaaaga
     1081 ccatgcgaga agcaaagctc cttcctcagt gtcagattaa caaggtaatt gacgggcgtc
     1141 catgttggaa tccgtcgtcg gcggcgattg gccgagggag gtggcggtca gtatccttat
     1201 tcagggccaa tgcatcgggg gaaggccggt ttatctgggc cggaattccc ccgtcgatga
     1261 aaaaagaaag gtgatcgaga gagcgaacct atgacgagag tattcgcgcc gttgataagt
     1321 atttttgcga aggcaaccga actggaagag cgcgaggtcg cgggccttct gtgcccgcct
     1381 ccgagtgccg agctcgggga tttcgcgttc ccgtgttttg tgcttgcgaa gcgtctgcga
     1441 aaatcgcccg ctcaaattgc cgcagaactc gcagaacgtg tgaattcaaa cgacaccatc
     1501 gcttcggccg ttgcggtggg gccctacgtg aacgttcggc tccatcgagc accgttcatc
     1561 agtacgttgc tggaggaggt acgcaaccag ggagagcagt ttggctcttc ttcgagcggc
     1621 aacgggaaat gcgtggtcct ggacttctca tccccgaaca tggcgaaacc gtttcacgtc
     1681 gggcatctca gcagcacgat aatcggcggc tctctttccc gtttgtttgc gtatcaggga
     1741 taccgcgtgg tgcgcatcaa tcatctcggt gactggggca cgccggtggg gcagcagatt
     1801 actgcgttca agcgttgggg ggacggaacg gagcttgaca gacaccccat tgcccatatg
     1861 gtggacctct accagcggtt tcacagggaa aaagccgaga accccgggct ggaggaagag
     1921 gcgcgcgagt ggtttcgaaa gctggaaacg ggtgacaaag aagcccggga gttctgggag
     1981 cacattcgtt ccgtttcgat tcaggagttg aaccgcgcct atgcgcggct gaatgttgag
     2041 ttcgatgcct accttggcga ggcgttctac gaggggatgc tgcaagagac cattgaggag
     2101 gtacagaagg caggaatcac ggaggtttcc gatggcgctc tcatcgtgcc gctcgattcg
     2161 aaagggatca agactcctct cattctcagg aagagtgatg gctcaacgat ttatgcaacc
     2221 cgcgacatcg cggcggcgcg gtaccgtgcg gaaaactatc gtttcgacct gtgcctttac
     2281 gtggttgccc acgaccagga gcttcatttc aggcagttgt ttgccactct cgaactgatg
     2341 gggtacgact gggcgaagag gtgcgttcac gtgcagttcg gccacgtgca cgggatgtcc
     2401 acgcgcaaag gtggcgccat cctgctggac gagttgctcg accgtgcaac agagaaagtg
     2461 cgcgaaactg tgatggcgaa ccagaccctt tttcgcgatg ttcctgcggc ggaaatagac
     2521 gaggtgtgcg aggcggtggg tgtcggagcc gtaattttcg ggaccctgaa ccatcgtcgc
     2581 acgaaggacg tggagttctc gtgggataac gcactgaatt tcaacggcga aacgggaccg
     2641 tatgtgcaat atgcgcatgc acgtctctgc agcatcctga gaaaggccgg ggacgagcca
     2701 cgcggggcag cgaacgtcga gctcttgacg cacgatgcgg agtgggcgct tgtgaaggag
     2761 ctcgcgcggt tccctgaggt ggtcgaggaa gcgtgtgccc agtatgaacc ttcatgcgtt
     2821 gctcgctacg ccgtagacct tgcccgtgcc tttacggtgt tctacgatca gtgccgcgtt
     2881 ctgggggagg aacccgagct ctccgcagcg cgcctttcgc tggtaaatac cactcgcgtc
     2941 gtgatctcca gtgcgcttca tctgctgtgc atgaaaacac ctgaacggat gtgatgacta
     3001 tgtccaccct gacgctgcct gagctccccg tatacgtgtg ccgtcgaacg gcacggccca
     3061 tctccgtaga cggaatcctc gatgaacccg actggggagc ggcggagccg atgcgcctcg
     3121 tccggaccga atcaggggcc gtgccggaac agccgacgag cgtccgggtt ctctgggatg
     3181 acgaatacct ttacgtagcc tttgagtgtg ttgataccga tgtgtgggcg acgattgaag
     3241 agcgcgatgg tgctttgtgg gatgaggaag tggtggaagt gtttgtcgat gacgacagcg
     3301 attccaggac gtatcttgaa tacgaaatca acccgttgaa cgcgctcgtt gatctctatg
     3361 ttctcaacag gactggtcga cgggacgcga tcaagttcat gctggactgg aactcttccg
     3421 gcattcgcca tgccgtgcgg gtggacggcg acccgcggag aaggggaagc agcgatcgag
     3481 gatggacggc cgagtgggcc atcccgtttg gcgatttcac cacggcgccg aacattccgc
     3541 cgcggaacgg ggatgcgtgg cgcatcaatt tctaccgcat agaccgaggc gaccgggacg
     3601 agtattccgc gtggtcaccc accggcgcga tcaatttcca cgtgccggaa cggttcggga
     3661 cccttgtctt ctcaacggaa acggtgacgc gcaaggatca ctgaaatgga ggagcgaccg
     3721 atgaacgata cacctctcgt attgtgtcgg cggacggctg aacagatcac cgtggatgga
     3781 gtcctgtcag aacgttcctg ggctgcagtg gaaccgataa cgctctgccg aacagacacc
     3841 ggagatgcag ccagattccg aaccgaggtc cgcctcctct gggatgacac ttacctctac
     3901 gccagtttcc actgcgtgga tcccgacatc tgggcgaaca tggtcctgcg ggacgcgcca
     3961 ctctgggagg aggaggtcgt agagatgttc ctcgatccga atcggagtgg aaacgcgtat
     4021 tttgaattcg tagtaaaccc tttgaacact ttgactgacg tcttcgtgct gaaccgatgg
     4081 ggccaattca aaccacttcg tgactggaac gcagaaggtg tgaaacacgc cgttgtagtg
     4141 gatggtgatc cctccgatcc gctttccgag gacaggtcct ggaccatgga gtgtgcgatt
     4201 ccattccggg aactggttac ggctccgaat atcccgccca aaagtggcga ttcctggcgt
     4261 gggaatttct accggattga gcagggatac gaatggcagg aatacacggc atggtctccc
     4321 acgggcgagg taaatttcca ccatccggag cgtttcggga cgatcgtgtt cacctctgag
     4381 actgcatagg cacgagagga tcgcccgggc gctgattgcg caaccatggc gcgtgcccgg
     4441 gttgccccgg aatcctgttt tcatgaaacg cctttggctt tttctcattc tggcacccca
     4501 cgtggcatgc gcgggaggac ttgccgggct gggattcaca gctaatgtcc cgaggcagac
     4561 cctcgggggt gtgatcgtgt attcgtggac ctatgccgac gtgaaggtat cgcggaattt
     4621 tctcggtatc tggcgcgata cgtacgagag tatcagtgtt ccggaggcgg tgtatgagtt
     4681 cggtgacagc ctcatcaact ccgcccgtac ctacaaaact gtcgacgcgg gtctgcaggc
     4741 gcggttgtcg catcgtacca ccgcttacct gggtcttggc gtaacggaaa cacgccacct
     4801 ttaccagttt ctggacgatt ttcgcatatt ggggagaaac gggcagtact gggtcgagca
     4861 cgaagtggac tacggtatca acgtgaacgg aggggtgtac ctccgcgttg ccgggccgtt
     4921 gtatggccag attggtttca atacgaaccc tcgcgggctt gaccttggcg tggccatcat
     4981 ctttgcatcc ccgtgaggga gtcaccccgg cgcattcggg caatggtatg cgcgggtttt
     5041 gttcgcacgt cgtaattgca gcggctgcca cggcggtgtt cttctcgtgg ctttgtggct
     5101 gtggcattct ctcgtccacc ggtacgcaga accctttatg gggtgtctgg tccggacctt
     5161 acccctcttc tctcggtacg caggcagaca gctcacgctt cgcgttcacg tcgaaacggg
     5221 tgtacctgtt tttcgcgctg aaagcggacg gtactatcct cgcccgagaa ttcggctact
     5281 acagctcaaa cgggtacctg atgattcttt cctcggaccc gggcggaaat ctcgaccggg
     5341 aagagatttt ctatgagttt gtcggggagt cgctcgtggc gaccatccgc ggacaacgag
     5401 tggtgtaccg gcgcgtggga ggcgtggagt gggcggatga tctgggatct gcagctcatc
     5461 tcaaggaggg tgcggtcggg cggtgagtgt gtgggtgagt cgaggcggtg acaaactttc
     5521 cgccgcattc caggcctgga agtcggttct tcctcggctt gagggtgctg ttgcggcgga
     5581 tttcggttcc aatgtcggtg gatttgtgca atgccttctt gcgcaaggga tcactcgagt
     5641 ctatgccatc gacaccggat acggtgttct cgcgtgggaa ctgcgcaacg accctcgagt
     5701 gcgcgtcatg gagcggacaa atgcgctgca tgttgtgctt ccagaaaagg ttgacctcgt
     5761 cacgatcgac gtcgcatgga cgaaacaacg gcttattctc cccgcggcgt tccggcttct
     5821 gagagacggc gggcttgtcg ttacgctggt caagccgcag tacgaggcga ctcagaagga
     5881 aaggcggggt ggagttctgc cgggcgaacg cgtgccggac gttctcatgc gggtgcgggc
     5941 agaagttcgt tccgtgggcg gagaaatcct cgcggaactg gagtcaccca tcaaaggagc
     6001 gggagggaac acggagttcc tctggctggt tcgtccagga aacgccacgt tctaagcgcg
     6061 tcgttgaccg gccggttccc ttctggcgcg ggcaaatgga aagggggaca tcgtcggttg
     6121 acgtccccct ttctcaggac gcgtgatcgc tctgtgtcac cctactttgc gagccccttg
     6181 atgtactccg cgtcgcgaag aaggacgttc tttctttcct caatcggaaa tttccccaga
     6241 gattcgtcct cgatgcagag atcacgatcg tacccggcat ccctcaggta gccgacaacg
     6301 atggtgtgat cgacgtcgcc gtccggtatc gggcagacgt acttgccgta ttcccacccc
     6361 atttcgcggg tgatgtttcg cttgtcttcg gggtagttga tgttcttgat gtgggtgtgt
     6421 ttcgccagcg gggcaaattc tttgatgacc gcgtacgtct tttccaacgc gtggccgcgc
     6481 cagtagaaat tcccggtatc gagcgtcata cctatgcggc tcgaccccac tccatcgaac
     6541 acgccgtgca ggaagttcgg gtcgtttccc tgatagccat ggttctcaat tcccatgtca
     6601 accggtgcat ccggggtggc atcgatgagc gcgcgtatcc ctttgacgaa cttctccacg
     6661 cgcacgtcga agggaagctc ctgttgtcct ttcatggcgc tgtcaatccg aaccgccttg
     6721 acgccgagcg catgtgcaac tttgagcgcc ttcgtgcacc atgcgagttc gtcttcaagg
     6781 tcgtccgcgt tgagattgtt tgccagaaga aacgcgctga tcttcacgtt gtactgatcg
     6841 aggtgcttct tgtattccgc gatttcggcg cttgtcgtca cggggaaaaa cacggtcttg
     6901 tccgtgagcg acggaagcga tacctgtctc gtgaagttcg cttcgatcgc cgaaagaccg
     6961 agcgccttca ggcctgtgat gatgtcggga tacgccgact gaaccgtatc gtctctgatt
     7021 gatgcataca tggtatgccg cctttgtgaa gatggtgaag gtcgggccgg ttcgagatac
     7081 ggcccgttcc gcggatgctt gataaaagat tcgtcaccgc ggcagagcga tgcaatgcct
     7141 ggtgcccggg aggctacccg aagaagtaga tcatcgtgcg gatgtcatgc cctgcgccgg
     7201 ggaaccagat ccagtccact gcgaggcaga aaatcgcccc tgcaacgtgt ccggccacaa
     7261 ggccgatgaa aaacgggtaa caccggcggt acagactcag accgccgatt ttgtggacga
     7321 tgattttggt agcccatgtg atgaatacac ccgaccagat cgcactgtgg tagaacgtgg
     7381 ttgccgtgca gaatccgatc gggttgagt
//
LOCUS       DAOTNG010000146         7288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8949_length_7288_cov_4.416839, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000146 DAOTNG010000000
VERSION     DAOTNG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8949_length_7288_cov_4.416839"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="P5179_08165"
     CDS             complement(<1..994)
                     /locus_tag="P5179_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95231.1"
                     /translation="MESETTEVTTDAYGQLRVVSSGALGSWRNIILDNRFAAGMSSLR
                     YDFRGTGEWKNTSPVGVNLDGAVSARRFHGANSASRSSYIETSGEVAALSPLPHAYPG
                     TWGSVHSVRGVWYDRTSSVYTNAMSHWHGLALETTRDLSTWTTKAGVEHEFVPDSSEL
                     GYWGFSGSSFAMWVPSLDWLVSGGVIAAWKDYPAPGTHPDESSATLHARVTRRLNPSW
                     SLETGINGAVLAFSPADDVFPSTRNMELLAEAKWEPGLVSTRFGAGAGMQQSPSSPAD
                     EYSERFLQAGCNLISRTSGFSDVSIRAGKRTYRHPSAAYSSDYVFGEFTALVSGA"
     gene            complement(1193..1597)
                     /locus_tag="P5179_08170"
     CDS             complement(1193..1597)
                     /locus_tag="P5179_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95232.1"
                     /translation="MASSTKRLLRAGWSLLVAIVCVSPACSPPARSPDVFPDSRSDES
                     ALEVASDLPASANLDEDFALWRETYELTRSASSDSICIAKADAIASEAETQWLLGDRE
                     QAEVLWGEAFDLLSGTCADTILVEPVHPPDSR"
     gene            complement(1632..2885)
                     /locus_tag="P5179_08175"
     CDS             complement(1632..2885)
                     /locus_tag="P5179_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95233.1"
                     /translation="MRMRSLLHVAVIAGFGIFVSPRGAVSQGGGGPQQGVVAGDPLPA
                     LATEWQQSFDAARQTNNSEALSLLSRAKTLLNQAETFRRRGETARAAANADEARLLIL
                     RANQILRTPPGRSPQIDATLRDAETLMRNAVAQGAPPDQTAQVKLLLDQARKAQTEGR
                     HDAALRLSATAQTQARQLWQRAVASRQLRQRCRVLESAAEPMVRRARTMADANGGDAV
                     KSAAERAQQFLQMAQSTDEATHPAAKARLLERAMQNAEAVLKTLDAATYSAHLASRSI
                     ADADAALARADEVASGDASGNDVNGELRRGRVVIANARQELASGNSIRARALAEEARQ
                     IAEQVLRSALGEVTEASVRAALQQTDALIERASAASGNETDPLLVEARNRQADARRML
                     ADGDLRRALAQTRIAARLAQRINAR"
     gene            complement(3076..4797)
                     /locus_tag="P5179_08180"
     CDS             complement(3076..4797)
                     /locus_tag="P5179_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRS95234.1"
                     /translation="MGFTTYARAALILATTCTTFSSPAEARRGGPESCPLVISEIMVN
                     PSGTETSREFVELFNVGTGSVNLGDYVVGDTNETDAIIAVSSGLVLKPGRFALILDPG
                     YFSANEPYPDIPAEALVVTIGDAAFGRGGLVNSRANTVVLRSSGGCEQRVSYLPEAVF
                     PGLSLEKIDVRGPDTPDNWAVSLVPDGTPGRPNSRRRNACDVSLRNASASPDTLHPPE
                     QLTVRFDVLNSGTDTTGPFPVILFADRDRDSVLSAAEDTLVRWNVRSLQPAETFAGVE
                     TIPVSRPFSGPLMLAAMLPGDENPRDNTQALRCVIGLPARSIVINEIMAYPSDGEGQW
                     VEVLNAGADPLSLAGSLLKTATGSGSADYGPLLYPGDYLVIAALPEGVRTRYGIGTPL
                     CGVTGGFPYLSRSASSSRRVSFLDATGTSIDEAHYPVPEQGRSLERADSASDGSNPAS
                     WAPSWAPARATPGLRNSASYPLPRGFDCMADPIPATDTVGIRIITPEHGSSVSLTVFD
                     MAGREIRTLLTRERLPAESHVRWDGTDGSGRQVPTGVYILHIEVVSPDEGKHFRRSQP
                     LVIVRRR"
     gene            complement(4764..6878)
                     /locus_tag="P5179_08185"
     CDS             complement(4764..6878)
                     /locus_tag="P5179_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95235.1"
                     /translation="MNGSNFPSVGWEITFREGWWLWSALGLALILLAVFSYRRTIPPL
                     PPRRRALLFGLRLLALCAFILAILNPRLERRGMEPRPRRIALLVDESRSIARAGQESA
                     VRSILASAQMERVSALADVVALGFADTVRVLDEATLSFRGPATEIGEAILSQRRVFPP
                     PDAIILITDGSNTSGSEPVRAAQEAALPTYVVGVGNPHPEPDARIVGVAAPPYAVEGK
                     PVSVTVAWEHTIPGMRASTMRLFHQGREITRAIVLPPETGARADVDLTFTPSARGMQT
                     YTVELEGVPGESRTENNRRTFSLDVVKAKKRALIIAGTPSADVGFWNRFFGAREDYDP
                     VIWYATPFRRVAPLSPDSIAGTDLIVWLDPAGNTLPPVTQDAVARAVEEGCGLLCVPG
                     TNSVSPRLREILPVELTGTRFEPGQFEVRLAAPLFAHPISGMDPGFAEWSSWESVPPL
                     LGLVSGLRPRQGATVLVTGDAGPVAVAGHAKKGRVLVFGGAGYWRWDILPRGMGIENP
                     AGTAFWKAAIRWLISREASQMVRVQTGRPFYRLGEPVRVDIFVSDEASKPVDNASVIV
                     ATDGPDNWTGRATLSGPGRYTVWIRGLLTGAHTMRAEAFSGTTSLGTSETRVLVDASS
                     AEDDIVQMQTDVLSRIASAHPGSRFVTARDAEQLLASIPLSPVFEYSERTVSLGSAGW
                     VLCGIILLLTGEWGLRRTHGLL"
     gene            complement(6898..7131)
                     /locus_tag="P5179_08190"
     CDS             complement(6898..7131)
                     /locus_tag="P5179_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95236.1"
                     /translation="MGRRKDYPLPSSVGDEKKWAKGEWWEWLAIFFSIAALWPVILRW
                     DGIIWDLALFGALVLMIVVFLRRARRMKRSWKQ"
BASE COUNT         1431 a         2270 c         2206 g         1381 t
ORIGIN      
        1 gtgctccgct gacaagtgcg gtgaactcgc caaatacata gtcgctggaa taggctgccg
       61 aaggatgtcg ataggtcctt ttgcctgcgc ggatactcac atcagagaag ccggaggtgc
      121 gcgaaatcag gttgcaaccg gcttgaagga aacgctcgga gtactcgtct gccggggaac
      181 tgggcgattg ctgcatccct gcgcccgctc caaaacgcgt gctgacgagg ccgggttccc
      241 acttcgcttc ggcaagaagt tccatattac gggtgctggg gaacacatcg tccgcagggc
      301 tgaaggcgag gaccgcgcca ttgatgccgg tttccagcga ccacgaaggg ttcaggcgtc
      361 tcgtcacacg tgcatgtaat gtcgcggagc tctcgtcggg atgtgtcccc ggcgccgggt
      421 agtctttcca cgccgcgatc acaccaccgg ataccagcca atcgagtgaa ggcacccaca
      481 tcgcgaagga actgcccgag aacccccagt accccagttc ggacgaatct ggaacaaact
      541 catgttccac accggctttc gtggtccatg tgctgagatc tcgtgtggtt tccagcgcca
      601 gaccgtgcca gtgcgacatg gcgtttgtgt acacggacga ggtccggtcg taccacactc
      661 ctcgaaccga atgaacagac ccccaggttc cggggtacgc gtggggaagt ggcgagagag
      721 cggcaacctc tcctgacgtt tcgatgtacg aacttcgtga cgcgctgttc gcgccgtgaa
      781 agcgcctggc gctgactgct ccgtccaggt tgacgccaac cggcgaggtg ttcttccatt
      841 ccccggttcc ccggaaatcg taccgaagtg aactcattcc cgcggcaaac cggttgtcca
      901 gaatgatgtt cctccaggag ccaagagcac cggacgacac cacgcgcagt tggccgtacg
      961 cgtccgtcgt tacttcggtg gtctcggact ccacaatccg aaacccttct cccgccacct
     1021 ccgtggctgc gtgcaattcg tatgtggtct cccacaaatc gcctgcgcgc gcaacacacg
     1081 ggcctgacgg caaaaaagcg accagaaaga ggaggcgaat gagactcgtg tggggttgtg
     1141 ccacaaacgc gcccttggag tggataatcc agaacggcca gcaggcccgt gtctaccggg
     1201 aatcaggggg gtgaaccggc tccactagga tagtgtctgc acacgtgcct gacaagaggt
     1261 caaacgcctc accccacagc acctcggcct gctcccgatc tcccagcagc cactgcgttt
     1321 ccgcttcaga cgcaatggca tccgccttcg cgatgcagat ggaatcagac gaggcgctgc
     1381 gcgtcagctc gtacgtctcg cgccagagag cgaaatcctc gtccagattg gctgaagccg
     1441 gcaagtccga agcaacctca agagcgcttt catcgcttct gctgtcgggg aacacatcag
     1501 gagagcgggc aggcggagaa cacgccggac taacgcagac gatcgcgaca agaaggctcc
     1561 atcccgcgcg gagaagccgc tttgtggacg aagccatgaa cccgggccgc ggtgatccgc
     1621 acgcgatcgt gtcagcgagc attgattcgt tgggcaaggc gagcggcgat tcgtgtttgg
     1681 gcgagggctc tgcgaaggtc tccgtccgcg agcatgcgtc gcgcatctgc ctgtcggttt
     1741 cgtgcctcta cgagcaacgg atctgtctcg ttccccgatg ccgcagaggc gcgttctatc
     1801 aaagcatccg tctgctggag tgccgcacgt actgacgctt ccgttacttc tcccagtgcg
     1861 ctccgcagaa cctgttcggc gatctgacgc gcctcttccg caagtgctct cgctcggatc
     1921 gagtttcccg atgcgagttc ctgccgggcg ttcgcgatca ccacacggcc tcggcgcaat
     1981 tctccgttca catcgttccc gctggcgtct ccggacgcga cttcgtccgc ccgcgcaagc
     2041 gccgcgtccg cgtcagcaat gcttcgcgac gcgagatggg cggaatacgt tgcggcatca
     2101 agcgttttga gcaccgcttc agcattctgc atcgcccgtt caaggagtcg ggcttttgcg
     2161 gccggatggg ttgcttcatc agtgctctga gccatctgaa gaaactgctg tgctctttcc
     2221 gcagcgctct tcaccgcatc ccccccgttg gcgtcggcca tcgtgcgagc gcggcgcacc
     2281 atcggctcag cggcggattc cagtactctg catcgctgtc tgagctggcg cgatgctacc
     2341 gcgcgctgcc agagctgacg ggcctgagtc tgggcggtgg cggagagtcg caacgcggcg
     2401 tcatggcggc cctcggtctg cgccttgcgc gcctgatcga gcagcaattt cacttgcgcc
     2461 gtctggtcgg gcggagcgcc ttgtgcaact gcattccgca tcagagtctc ggcatcacgc
     2521 aacgtggcat ctatctgggg ggaacgacct ggcggggtcc tgaggatctg gtttgcccga
     2581 agaatcagga gtctcgcctc gtcagcattt gccgcagcgc gcgcggtttc acccctgcgc
     2641 cgaaaggttt ctgcctggtt cagcagcgtt ttcgctcgtg acagaagaga cagcgcttcg
     2701 ctgttgtttg tctgacgtgc tgcatcgaac gactgctgcc attctgttgc cagcgccggc
     2761 agaggatctc ctgcaacaac gccctgctga ggaccacctc cgccctgcga aaccgcaccg
     2821 cggggagaga cgaatatccc gaaacccgcg atgacggcaa cgtgcaaaag gctccgcatt
     2881 ctcatctcat tcaactcctg cccctttcgg gacaatcctg ccgcaactcc cgtgccggca
     2941 aaacggcggc ggccagcgat gcaggaaaca gcccttctca agggcaacac gtgtggagtg
     3001 cgggtcttcg cccggcgtac acaacgtacc aacaggtacg ttcttgccga cgaaaggcgg
     3061 tctcttctcc atcggtcatc tgcggcggac gatcaccaac ggttgcgaac gccggaaatg
     3121 cttgccttcg tcgggtgaaa caacctcgat gtggagaata tacacaccag tgggaacttg
     3181 acggccgctc ccgtccgttc cgtcccagcg gacgtgcgat tctgcgggaa ggcgctcccg
     3241 ggtgagcagg gtccgtatct cccgcccggc catatcaaag acagtgagcg agaccgaaga
     3301 cccgtgctca ggtgtaatta tcctgatccc gacggtgtcc gttgcgggta ttgggtcggc
     3361 catgcaatcg aaccctcggg gcagtgggta ggatgcgctg ttgcgtagtc ccggggttgc
     3421 cctcgccggc gcccacgatg gtgcccatga tgcggggttg ctgccgtcgc tggcggagtc
     3481 agcccgttcc agtgaacggc cttgttccgg aacgggataa tgtgcttcat cgatggaggt
     3541 gcctgttgcg tccagaaagc tcacgcgccg cgacgacgaa gcacttcggc tgagatacgg
     3601 aaaaccgccg gtcacgccac acagaggtgt gccgattccg tatcgcgtcc gaacgccttc
     3661 cggcaatgcg gcaatcacaa ggtaatctcc cggatacaac aacgggccgt agtccgctga
     3721 accgcttccc gtagcagttt tcaataagga ccccgcgagt gacaaagggt cggctccagc
     3781 gttcaggact tccacccact ggccttcgcc gtcggaggga tacgccatga tctcgttgat
     3841 aacgatggat cgggctggga gcccaatgac gcatcttagt gcctgcgtgt tgtccctcgg
     3901 gttctcgtct cccggcagca ttgcggcgag catcagcggg cccgaaaatg gtcgtgacac
     3961 cggtatggtt tctaccccgg cgaacgtctc ggctggttgc agagacctca cgttccaacg
     4021 gacgagcgtg tcctctgcgg ctgagaggac agagtccctg tcgcggtcag cgaacaatat
     4081 gacgggaaat ggccctgtgg tgtcggttcc agagttcagg acgtcgaatc gtacggtaag
     4141 ctgttcggga ggatggagag tatcaggact tgcgctggca ttcctgaggg aaacgtcgca
     4201 cgcattcctg cgacgcgaat tcgggcggcc cggtgtgcca tcgggcacaa ggctgactgc
     4261 ccagttgtct ggcgtgtcag gtccccgcac atcaatcttt tccagcgaaa gccccgggaa
     4321 cacggcctct ggaaggtacg aaacgcgttg ttcacaaccg cccgaagaac ggagcacaac
     4381 cgtgttcgcg cggctgttaa cgaggcctcc tcgtccgaac gcggcgtctc cgatcgtaac
     4441 gacaagagcc tcagccggaa tgtcggggta gggctcgttt gcgctgaaat accctggatc
     4501 cagtatcagg gcgaaccgcc ccggtttgag gacgagcccg gacgagaccg cgataatcgc
     4561 gtccgtctcg tttgtgtcgc caacaacgta gtcaccgaga ttgaccgagc cggttccgac
     4621 gttgaacaac tcgacgaatt cgcgagaggt ttcagtgccg ctggggttga ccatgatttc
     4681 ggagattacc agggggcagg actccggtcc gccgcgccgg gcttccgcag gagaggagaa
     4741 cgttgtgcac gtcgtagcca gaatcagagc agcccgtgcg tacgtcgtaa accccattcc
     4801 cctgtcagca aaaggatgat gccacataga acccacccgg cagagcccag cgaaacggtg
     4861 cgttcgctgt attcaaaaac gggggataga gggatcgatg ccaatagctg ctccgcatcc
     4921 cgtgcggtga cgaatctgga tcctggatgc gcggacgcga tccgcgacag gacatccgtc
     4981 tgcatctgca cgatgtcatc ttccgcgctt gaagcgtcga caagcacacg ggtttcggag
     5041 gttcccaggg aggtggttcc tgagaatgcc tctgcgcgca tggtatgtgc gcccgtcagc
     5101 agcccccgta tccacaccgt gtacctgccg ggaccggaga gggttgcccg ccccgtccag
     5161 ttgtccggcc cgtccgtggc cacgatcact gacgcgttgt ccaccggctt tgaagcctca
     5221 tcggagacga atatgtccac gcggaccggc tcaccgaggc ggtagaaggg gcgtcctgtt
     5281 tgaacccgga ccatctggga cgcttccctt gaaatcaacc aacggatggc ggccttccag
     5341 aacgcagttc ccgccggatt ctcgatcccc attccccggg gcaaaatgtc ccagcgccag
     5401 taccccgccc ctccgaacac gaggacccgc cctttttttg cgtgtcctgc cacggcaacg
     5461 ggaccagcgt caccagtgac gagaacggtt gcaccctggc gtggcctcag tccggagacc
     5521 agtccaagta acggcggaac tgattcccag gaagaccatt ccgcaaatcc ggggtccatt
     5581 cctgagatgg ggtgtgcgaa aagcggagcc gcaagcctga cctcgaactg acccggctcg
     5641 aatcttgtgc cggtcaactc gacaggcagt atttcacgca ggcgaggcga cacgctgttc
     5701 gttccgggga cacaaaggag cccacagcct tcttcgacgg cacgcgctac cgcgtcctga
     5761 gtgacaggag gcagtgtgtt cccggcaggg tcgagccaga caatgaggtc tgtccccgca
     5821 atactgtccg gggagagtgg agcaacgcga cgaaaagggg ttgcgtacca gattacggga
     5881 tcgtaatcct cgcgtgcgcc gaagaaacga ttccagaagc cgacatcggc gcttggagtc
     5941 cccgcgatga tgagggcgcg ctttttcgcc tttacaacgt ccagcgaaaa cgtgcgcctg
     6001 ttgttttctg tgcgagattc cccggggacg ccctcaagtt ccacagtgta ggtctgcatt
     6061 ccccgtgccg agggtgtaaa cgtcagatcc acgtcagctc ttgcgccggt ttcgggaggc
     6121 agaacaattg cccgggtgat ttccctgccc tggtgaaaaa ggcgcatggt ggaggctcgc
     6181 atgccgggta ttgtatgttc ccaggcaacg gtgacggaca ccggtttacc ctcgaccgcg
     6241 taagggggcg cagcgacacc aacaatgcgt gcatccggct cggggtgtgg attacccacc
     6301 cctacgacgt atgtcggaag ggcggcctcc tgtgcggccc ggacgggctc cgacccggag
     6361 gtgttggatc cgtccgtaat gagaatgata gcgtcgggcg gcgggaacac gcgccgctgg
     6421 ctcaggattg cctcaccgat ttccgtcgcg ggcccgcgga aggacaacgt ggcttcgtca
     6481 aggacgcgga ctgtatccgc aaagcctaag gcgaccacgt ccgcgagagc tgacacgcgt
     6541 tccatctgtg cgctggcaag gattgagcgc acagccgatt cctgcccagc acgcgcgata
     6601 ctccggcttt cgtcgaccag aagagcgatg cgccgcgggc gcggctccat cccccggcgt
     6661 tccagccgcg gattcagaat cgcgagaatg aacgcacaaa gcgcgagcag ccgcaggcca
     6721 aagagcagcg ctctgcgccg tggcggaagg ggtgggatgg ttctcctgta ggaaaaaaca
     6781 gccagcagga tcagcgcgag accgagtgcg ctccacaacc accacccctc acggaaggtg
     6841 atttcccaac cgacggaggg gaaattggat ccgttcatcc gaggagtgtc gtgccgttca
     6901 ttgcttccac gaccgcttca tacggcgtgc ccttcggagg aagaccacga tcatcaacac
     6961 gagagcgccg aagagcgcaa gatcccatat gatgccatcc caacgaagga ttaccggcca
     7021 cagcgccgcg atcgagaaga agatcgccag ccattcccac cactcgcctt ttgcccactt
     7081 tttctcatcc ccaaccgaag aggggagagg ataatccttt ctgcgtccca tttgcggcat
     7141 cctcaggctt ccagcggcgc gaccgtcaat ccgtcggtgt ctacgtgggt cacctccgcg
     7201 cggctcgtaa tgccatgtct tgcccatgcc tgctccatgg cgcgggccac ggcgtccgat
     7261 gagtggatcg ccagcgcgac tatcgcgg
//
LOCUS       DAOTNG010000147         7257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_8992_length_7257_cov_5.302833, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000147 DAOTNG010000000
VERSION     DAOTNG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8992_length_7257_cov_5.302833"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="P5179_08195"
     CDS             complement(<1..849)
                     /locus_tag="P5179_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRS95237.1"
                     /translation="MVRAFASAALRARAVIFLACVALAGVAVNPMVLRSTENPANVPT
                     QGNGNAALTAGAPASGGPFRLENKSYPPGRTLLVGKAGQVLEEPVRFLVTDTAGVPVA
                     GVPVVLEIVSRPKGAEDAEIASPLVSDSAGFVSANLSLGKAPGDYVISARVASMAGEM
                     PHVSAHALKGNWWLMVVMGIVGGLGMFLFGMELGAGGLQKVAGNKMRDILAKLTTNRV
                     MAVVAGAIITAVVQSSTATTVMVVGFVNVALMNLTQAIGVIMGANIGTTLTVQLIAFK
                     ISDYALL"
     gene            complement(969..2942)
                     /locus_tag="P5179_08200"
     CDS             complement(969..2942)
                     /locus_tag="P5179_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS95238.1"
                     /translation="MGADAVFVNSLGMRFVHIPAGSFLMGNDRPLPDELVRLPNRRYG
                     DFDERPIRLVRLTKPFKMATTEVTNAQFEAFDPSHRQLRGKLGFSTGDDEAAVFVNWF
                     EACAFCEWLSRREGLPYRLPTEAEWEYACRAGTTTAYHTGDSLSAEFHKNVGVKWFPD
                     PSRGADEQEIPALTVGQTPPNPWGLYDMHGNVEEWCHDWYGPYDAHRPNDPVGALDGN
                     FRVTRGGSHSTELYYLRSSNRMGTVPEERSWIIGFRPVIGEMPSTNAIPGPIQLHESN
                     VKPEIPRDITRGPDPSKPFFCGPRTYVKVPPDSYGPMFSRHNHVPAIVACPNGDLLAM
                     WYTCMEEAGREVATLASRLRWGKDEWEPASLFWDAPDRNDHACALWRSKSGRLFHFSG
                     LSVAATWGPLAVVMRTSDNSGATWSKARLIIPEHGVRHMPIAGVIQLSDGTIVLPCDA
                     VTAGHGGSALWMSEDEGDTWYDAGGTIAGIHANVVELSDGRLMALGRGDDIDGRMPMS
                     LSEDKGKTWTYSASPFQPIHGGQRLVLFRLQEGPLLFVSFANDPLEITDATGTRRSIT
                     GLYCAVSFDEGATWANFRVMSDDGLPRPVEAFDGRPNILSASQSEVAGYMTVCQADNG
                     LIHLISSRNHYVFNLAWLKEPPPGEQHVRQVKL"
     gene            complement(2960..3688)
                     /locus_tag="P5179_08205"
     CDS             complement(2960..3688)
                     /locus_tag="P5179_08205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS95239.1"
                     /translation="MRYQIVFWDSGGTIFGPGRDGPLAEKPSPREVREGRVHRVSLAL
                     EMFGHRAPSDLGRVLQDTSSELQEKLGARYSLERLASGVYERLSLPVRPEETILVADA
                     IGGPRYRTWLWDGVADALKALAEAGVRMGVIADTDWTGRMMSRALAGVGLREYFGPVI
                     CSCDIGVCKPAPEIFAAARELIPDVPSGSPVLYVGDNLEKDVGGAIAFGWDAAYHVTT
                     KGGSSGRAVLDFVQYQDLVTLVAG"
     gene            complement(3688..4422)
                     /locus_tag="P5179_08210"
     CDS             complement(3688..4422)
                     /locus_tag="P5179_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRS95240.1"
                     /translation="MRIVVHNVYWFQGCPSRWGGERVAAVPAVVDGLVELYASLSPDV
                     ICLQEVQDPSLVASAAERLGMASWIHAPGGVKTDYGGAILTRNPSKMRDLTSNGSQPP
                     HERVHLRAQVSNGEESLEIACVHLPSNRFAGSPEGGSRARVAEAERILRCSPAPDVVV
                     GDMNSRKDSDPYLLMKSYGYVNAGETDVAEDSPERKGDHIWLLPAVARRLKKFFVITG
                     EPYVQNDTDGTVWRLSDHRPLVADLL"
     gene            complement(4505..5293)
                     /locus_tag="P5179_08215"
     CDS             complement(4505..5293)
                     /locus_tag="P5179_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95241.1"
                     /translation="MGVVRDRLWIWGHEAGSHNEGWGVPSPSRMTPAEAAWYMGIPNV
                     IFVTYQGKPEPPYDIYARAFVPLKNVVWSIVGEAGRTSDAAITGVRDVAAKFPNITGV
                     MMDDFFHDPKPDGSLAPISHDRLQSVRATLDECGRKLDMWVVVYTHQLDLPIAPFLDK
                     CDVVTLWTWRQEDLVNVERNLERIEALAPGKRVVLGCYMWDYGNKCEMSVENMKLQCS
                     AGLRWLTSGRIHGMIFLASCVCDIELPAVEWTRRWISEHGDTVL"
     gene            5500..6555
                     /locus_tag="P5179_08220"
     CDS             5500..6555
                     /locus_tag="P5179_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95242.1"
                     /translation="MAFLTSKQRVLRTLARDEVDRVPVDVELTGEMRDKLLSHFGFRE
                     DEEIFRLFGRDFRRVTARYTGPECYAKNGQPSDVFGVASGGPTYADTIGFRPFARVES
                     VAEIESYSWPDPDWWDYSDIPAQCERFREYAITGGEWSPFFCQACNMTGIGRFLELML
                     DAPEIAEAILSHIVDFYVAGSRRYFEAARGGIDIFFVGDDYGGKNGMLMSPVLWRTLI
                     KPQLARLYALAGEYGVHMMQHSCGSIRPVIGDMIELGLEVLDPVQIAAADMDPEGLKA
                     EFGDRLTFHGGVNTEETLPFGTPADVFRETRYLMDTLGAGGGYVVCGSQYLQTDIPVA
                     NVIAMYKAAGSISQEII"
     gene            6563..6945
                     /gene="rnpB"
                     /locus_tag="P5179_08225"
     ncRNA           6563..6945
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5179_08225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6970..>7257
                     /locus_tag="P5179_08230"
     CDS             6970..>7257
                     /locus_tag="P5179_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95243.1"
                     /translation="MFLARSLPFVPERRWFRVAACAAFALAAVARIGLRIHFTDFRSP
                     ELWEFGEIARNLVQTGTFTYRSNGVPSAFMPPGYPLYIAFLYQVFGVGEIAH"
BASE COUNT         1466 a         2277 c         2052 g         1462 t
ORIGIN      
        1 cagaagcgcg taatcactga ttttgaatgc gatgagctga acggtgaggg tggtgccgat
       61 gttggcgccc atgatcacgc caatggcttg ggtcaagttc atcaatgcca cgttcacgaa
      121 tccgacaacc atgacagtcg ttgcagtgct gctctgaacg accgccgtga tgatcgcgcc
      181 ggcaacgacc gccatcaccc ggtttgtcgt gagcttcgcc agaatgtccc tcattttgtt
      241 gccggcgacc ttctgcaaac ctcccgcgcc aagttccatc ccgaagagga acatgccgag
      301 cccgccgaca atgcccatga caaccatgag ccaccagttg cccttcaacg cgtgggctga
      361 aacgtgcggc atttcccctg ccatgctggc aacccgcgcc gagatgacgt aatcaccggg
      421 tgccttgccc agggagagat ttgcggagac gaagcccgca gaatcgctca ccagcggcga
      481 ggcgatttcc gcgtcctccg cgcctttcgg tcgcgatact atttcgagca ccacgggaac
      541 gcctgcaacc ggaacgccag ccgtgtcggt aaccaggaac cgcaccggtt cttcgagaac
      601 ctgacccgcc ttgcccacaa gcaaagttcg tccaggcggg tacgatttgt tctcgagtcg
      661 gaagggtcca ccggacgctg gagcgcccgc cgtgagcgcc gcgtttccgt ttccctgtgt
      721 cggaacgttt gcggggtttt ctgtggagcg cagcaccatg gggttgaccg caactcctgc
      781 gagggcaaca cacgcgagaa aaatcactgc gcgagcacgc agcgctgcgg aagcgaaagc
      841 gcgaaccata cacacctccc ccggaaaagg gtatgtcgca caaggcggga agatcgcaac
      901 gcaatatgaa ggctgcaggt accgtcgtca atccgggtgg tctgacaata ccctaacatt
      961 ttccgccttc acaacttcac ctgtctcaca tgttgttcgc ccgggggagg ctccttcaac
     1021 catgccaggt tgaacacata gtgattcctt gaggaaatga ggtggatgag tccgttgtct
     1081 gcctggcaca ccgtcatgta cccggcgact tctgattgcg acgcgctcaa tatgttcggg
     1141 cggccatcga aggcttccac cggcctcggc aggccatcgt cgctcatcac ccggaaattg
     1201 gcccacgtcg caccttcgtc gaaggaaacc gcgcaataga ggccggtgat cgaacggcga
     1261 gtgccggtgg catcggtgat ctcaagtgga tcattcgcga atgagacgaa aagcagcgga
     1321 ccttcctgca accggaacaa caccagccgc tgtccgccgt ggatgggctg gaaagggctg
     1381 gcgctgtatg tccacgtttt tcccttgtcc tcggacaggc tcatcggcat ccgcccgtcg
     1441 atatcgtcgc ctctccccag cgccatgagg cgaccgtcgc tcagctccac cacgttcgcg
     1501 tggatgcccg cgatagtccc cccggcgtcg taccatgtgt cgccttcgtc ctcgctcatc
     1561 cagagcgccg agccaccgtg tccggccgtg actgcgtcac agggaagtac gattgttccg
     1621 tccgaaagct ggatgacgcc tgcaatcggc atgtgccgaa ccccgtgttc cggaatgatc
     1681 agccgcgcct tcgaccacgt agcgccgcta ttgtccgacg ttcgcatcac aactgccagc
     1741 ggcccccacg tggcggcaac cgatagaccg gagaagtgaa acaacctccc tgatttgctc
     1801 ctccagagcg cacacgcgtg gtcgttgcgg tcgggagcgt cccagaacaa ggacgccggt
     1861 tcccattcat cctttcccca tcgcaggcgg ctggcaagag tcgccacctc tctcccggcc
     1921 tcttccatgc acgtatacca catcgcgagc aggtcaccgt tcggacacgc aacaatcgcc
     1981 ggaacgtggt tgtgccgtga gaacataggt ccgtacgaat ccggaggaac cttgacatac
     2041 gtcctcgggc cgcagaaaaa tggttttgac gggtcggggc cacgcgtgat gtcccgggga
     2101 atctcaggct tgacgttgct ctcgtgcagt tggatcgggc cgggtattgc gttcgttgac
     2161 ggcatttcac cgatcaccgg ccgaaatccg atgatccagc ttcgctcctc gggaacggta
     2221 cccattcgat ttgacgagcg caggtaatac aactctgtcg agtgcgaacc acccctcgtc
     2281 accctgaaat tgccatcaag ggctcccacc gggtcgttag gccggtgtgc gtcatacggt
     2341 ccgtaccaat catggcacca ttcttccaca ttgccgtgca tgtcgtacag accccacggg
     2401 ttcggcggcg tctgccccac cgtcaatgcg ggtatctcct gttcatcagc cccgcgggag
     2461 gggtcgggaa accatttcac tccgacgttt ttgtggaatt ccgccgacaa ggaatccccg
     2521 gtatggtacg ccgtagtcgt gccggcccga caggcgtact cccattcggc ttccgtggga
     2581 aggcggtacg gcaacccttc tcttctggaa agccactcgc agaaggcaca agcctcgaac
     2641 cagtttacaa acaccgccgc ttcgtcgtcc cccgttgaga acccgagttt gccacgcagt
     2701 tggcgatggg aagggtcaaa cgcctcaaac tgagcgttcg tgacttctgt ggttgccatt
     2761 ttgaacggct tcgtcagtcg cacgagacgg atcggtcgtt catcgaagtc cccgtaccgg
     2821 cgattcggaa gcctcacgag ctcgtcggga agcggtctgt cattccccat cagaaacgaa
     2881 ccggcgggaa tatggacaaa acgcatcccg aggctgttca cgaacaccgc atcagcgccc
     2941 atcaagtcct cctcttcgtt caacccgcta ccagggtgac aaggtcctga tactgcacga
     3001 aatccagtac ggccctgccc gatgaaccac ccttggtcgt cacgtggtac gccgcatccc
     3061 agccgaaggc gatcgctcct ccaacgtctt tctcgagatt gtcgccgaca tacaaaaccg
     3121 ggctgccgga gggcacatcg ggaatgagtt cgcgtgcggc ggcaaaaatc tccggagctg
     3181 gcttgcaaac gcctatgtcg catgagcaga tgactggacc gaaatactcc ctcaatccga
     3241 cgccggcaag tgcgcgggac atcatccggc ctgtccagtc cgtgtccgca atcacgccca
     3301 tacgcactcc cgcctcggcg agcgccttga gcgcatcggc aacaccatcc cagagccatg
     3361 tgcggtaccg cgggccacca atcgcatctg ccacgagtat ggtttcctcc gggcggaccg
     3421 gcaaactcag gcgttcgtac accccggacg ccagtctttc aaggctgtac cgcgctccca
     3481 gcttctcctg gagctcggaa ctggtatcct gcagtacccg cccgagatcg gacggtgccc
     3541 ggtgcccgaa catttccagt gccaatgaaa cacggtgcac gcggccttcc cgaacctctc
     3601 tgggcgaggg tttttctgcc agtggcccat ctctccccgg cccgaaaatc gtgccgccac
     3661 tgtcccagaa aacgatctgg tatctcatca gagcaaatcc gccaccagag gacgatggtc
     3721 gctgagccgc cagacagtgc cgtctgtgtc gttttgcaca tatggttcgc cggtgatcac
     3781 gaaaaacttc ttcaatcggc gcgccactgc tggcaaaagc cagatatggt cgcccttgcg
     3841 ttccggcgag tcctccgcga catcggtttc gcccgcgttc acgtaaccat aagatttcat
     3901 cagcagatag gggtcactgt ctttgcggga gttcatgtcg ccaactacta cgtctggcgc
     3961 cggcgagcac cgaaggatcc gctccgcttc cgcgactcgt gccctgctcc ccccctcggg
     4021 agaacccgca aacctgttgc tcggaagatg cacgcaggcg atctcgaggc tttcctcccc
     4081 gtttgagact tgtgctctca ggtgcacccg ttcatggggc ggttgactgc catttgacgt
     4141 cagatcgcgc atcttcgaag gattccgtgt gagtattgct cctccgtaat cggttttgac
     4201 cccgcccggg gcgtggatcc aagaggccat cccgagtctc tccgcggctg acgcgaccag
     4261 actcgggtcc tgaacctcct gcaggcaaat aacgtctggc gaaagagatg cgtacagctc
     4321 gacaagccca tcgaccacgg ccggcactgc cgcgacgcgt tccccgcccc agcgggacgg
     4381 acacccctga aaccagtaga cattgtgcac cacaatgcgc atatacccat gcctttcccg
     4441 ccatgagacc gttacgcgcg accccagaaa tggaatcaaa cgtcgaatcc ggacgtgagg
     4501 acgttcacaa aacggtgtct ccgtgctccg atatccaccg gcgtgtccat tccaccgcgg
     4561 gcagctcgat gtcacacacg cagctcgcga ggaagatcat cccgtgaatg cgaccggacg
     4621 tcaaccaacg caatccggcc gagcattgga gtttcatgtt ctccactgac atttcgcatt
     4681 tgttgccgta gtcccacatg tagcatccaa gcactacgcg tttgccggga gccagcgctt
     4741 cgatcctttc cagattgcgt tccacgttca caaggtcttc ctgccgccac gtccacagcg
     4801 tcacaacgtc acatttgtcg agaaacggcg cgatgggcag atcgagctgg tgcgtgtaca
     4861 ccacaaccca catgtccagt ttgcgcccgc actcgtccag tgtcgcgcgg acgctctgca
     4921 gcctgtcgtg ggagatcggc gcgagacttc cgtcgggttt gggatcgtgg aagaaatcgt
     4981 ccatcatcac gcccgtgatg ttcgggaact tcgccgccac gtcacgcacc ccggtgatcg
     5041 ccgcatcaga ggtgcgtccg gcctcgccga ctatggacca gacaacattt ttgagcggca
     5101 cgaacgcccg cgcgtagatg tcgtacggcg gttccggctt cccctgatat gtcacaaaaa
     5161 tcacgttcgg gatacccatg taccacgctg cttccgccgg ggtcatgcga gacggagagg
     5221 gcacccccca tccttcgttg tggctccccg cttcgtggcc ccatatccaa agcctgtcac
     5281 gaactactcc catatcgtac gcctccctgt gagtcgaatc ggtcgccaat gccagaacct
     5341 agcctttcag aatgtattcc ccgcggatga cgccaccaac tgcatacgga acgcaatcgc
     5401 aggaacgagt tgagcgttgc gctctgtgga gagctgtctt tatcatcgtg ggcagccgca
     5461 acacaggcgt ctccgttttc aacaccggaa agcgcggata tggcctttct gacatccaag
     5521 caacgagttc tccgaacctt ggcgagagac gaggtggacc gtgttcccgt tgacgtggaa
     5581 cttacgggtg aaatgcggga caagctcctc tcccattttg gttttcgcga ggacgaggag
     5641 attttccggc tgtttggccg cgatttccgt cgggtaaccg cccggtacac ggggcccgaa
     5701 tgttatgcga aaaacgggca accctcggac gtcttcgggg tggcaagcgg cggcccgacg
     5761 tacgccgaca cgataggctt ccgtccattt gcgagggtgg aatctgtcgc ggaaatcgag
     5821 agctactcat ggccagaccc ggactggtgg gattattctg acattcccgc gcagtgtgag
     5881 cgatttcgag agtatgcgat taccggtggg gagtggtcgc cgtttttctg tcaggcgtgc
     5941 aacatgaccg gcatcggacg tttccttgag ttgatgctgg acgcaccgga aatcgccgaa
     6001 gcgattctct cccacatcgt ggatttctat gtcgccgggt cacggaggta tttcgaggca
     6061 gcccgcgggg gaattgacat ctttttcgtc ggagacgact acggcggcaa aaacggcatg
     6121 ctgatgagcc ccgttctctg gcgcacactc atcaagcctc aactggctcg attgtacgcg
     6181 ctcgccggcg agtatggcgt ccacatgatg caacattcgt gcgggagtat tcggcctgta
     6241 atcggcgata tgattgaact cggactggaa gtgctggatc cggtgcagat tgcggcggcc
     6301 gacatggatc cggaagggct gaaagcggaa ttcggggacc gcctgacctt ccacggaggc
     6361 gtcaacaccg aagaaacgtt gccatttgga actcctgcag acgtgttccg ggaaacgcgt
     6421 tatcttatgg atacactggg cgccggtggc ggttatgtgg tctgcggatc acagtacctt
     6481 cagacagaca tccccgtcgc aaacgtcatc gcgatgtaca aggcggcggg aagtatctcc
     6541 caggaaatca tttgacatcg gtgaagagag ccggacagcc gccgtgtatg cggaggaaag
     6601 tccgaacacc acagggcagg atgccgggca acacccgggc ggcgtgagcc gatggaaagt
     6661 gccacagaaa acataccgcc ggacgcgtcc tgtacgcgcc cggtaagggt gaaaaggcag
     6721 ggtaagagcc taccgcgcct tcggcaacga aggtggcagg gaaaacccca tccggtgcaa
     6781 ggccaaatag gagcggattc gaggctgccc gtctcgtcaa cacgctcggg taggccgctt
     6841 gaggagtgcg gcaacgcact tcccagatag atggttgtcc ggggagccgt cgtccgcagg
     6901 atgccggctc cttgacagaa ttcggcttac aggctctctt caccacctct tctcaaaccc
     6961 ggtgcgcaca tgtttctggc gcgctcgttg ccgtttgtcc cggaacggag atggttccgc
     7021 gtcgctgcgt gcgcggcttt tgcacttgcc gccgtggcgc ggataggact gcgcatacat
     7081 ttcactgatt tccgctcgcc tgagctctgg gaattcgggg aaattgcgcg gaatctcgtg
     7141 cagacgggta ccttcacgta tcgatccaac ggcgtcccat ccgcgttcat gccgccgggt
     7201 tacccgctct atatcgcgtt cctgtatcag gtcttcgggg ttggcgagat tgcacac
//
LOCUS       DAOTNG010000148         7232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9015_length_7232_cov_5.252334, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000148 DAOTNG010000000
VERSION     DAOTNG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9015_length_7232_cov_5.252334"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5179_08235"
     CDS             complement(<1..347)
                     /locus_tag="P5179_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95244.1"
                     /translation="MELLRRALLPCICGMICLAAAGCGRDKFGNALDKVLEQDTVTTI
                     GVIGVFGSPGNHHYVERDAALAAYLAKIADGKYTIITIDTAVANKVLSVVVQPTSNFA
                     DAKVVAKIGKAFG"
     gene            complement(497..1594)
                     /locus_tag="P5179_08240"
     CDS             complement(497..1594)
                     /locus_tag="P5179_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95245.1"
                     /translation="MTPRQRVHAALNRLPADRIPIFMWFHPVTARRLAAELDIPVSEL
                     STVMGDDVRQTWVGNNHAMEGIVHERDGETHRDDWGIEWVKSGHFNQIRSSPLEHASR
                     EEALAYQYPYDRVDSLMANMVPLLRYSDRYFIGCDVSPCLFEMICRLRGMEQATLDLA
                     EDPDLARTMLAEAGAFSRHLAEEACRRFRLDWLWTGDDVGGQLAMIMSPSLWRELVKP
                     RLKEIFAAGKTGGIPVAYHSCGAVRDIIPDLIEIGLDVLNPIQCNCPGMDPASLKREF
                     GRKLTFMGGVDTQGLLPQATADEVFRETRKLIEIMTEDGGGYILAASHTIPPETPSEN
                     IFAMYDAAGLSREAIADAAADARKRGAGLRS"
     gene            complement(1741..2685)
                     /gene="fmt"
                     /locus_tag="P5179_08245"
     CDS             complement(1741..2685)
                     /gene="fmt"
                     /locus_tag="P5179_08245"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRS95246.1"
                     /translation="MKPLRIVFMGTPEFSVPSLEKLASGGHHVVAVVTKPDKPAGRGR
                     AIQPPPVKRAAERLGLPVIQPPSLRDTDTHSRLEALQPDLFVVVAFRVLPEELLRIPR
                     LGAINLHASLLPKYRGAAPIQRALINGERETGVTVFLLEPTVDTGRILRQRPIAIGDD
                     ETCGELAARLSVTGAEELAAAVDDLARGAAVMIPQNDAEATTAPKLTKEDGRIRWNER
                     AEAIRNRVRALNPSPGAYALWKGQPFGILRAAVEEAPAPGDEAPGTILQADDRHGVRV
                     ATGDGILRLDIVQPAGRRPMEGAAWVRGAHPQKGERFE"
     gene            complement(2772..3290)
                     /gene="def"
                     /locus_tag="P5179_08250"
     CDS             complement(2772..3290)
                     /gene="def"
                     /locus_tag="P5179_08250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRS95247.1"
                     /translation="MALLPIRIYGDPVLRRVAEPVREFTPELRTLATDMIETMYAEPG
                     VGLAAPQVGVSIRMIVIDPTAGDQKGKAFALINPVVTRSGEHVVIEEGCLSVPDIRAD
                     VVRPERVSVDYQTIDGEPAHLDADDIVARIIQHEYDHLDGILFVDRISPVRRQLLRKQ
                     LKALAKSQGKRS"
     gene            complement(3321..3683)
                     /gene="yajC"
                     /locus_tag="P5179_08255"
     CDS             complement(3321..3683)
                     /gene="yajC"
                     /locus_tag="P5179_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRS95248.1"
                     /translation="MRVARLTIAGLMLTGGNAFAMGAQPSGGQAGSPWGMFVPLLLMF
                     VVLYFFMIRPQQKRQKEKEKMLEALKKGDRVLTLGGIYGDIQQVKDSVVVLRIAENVK
                     VEVQRSAISSVVESAASE"
     gene            complement(3730..4875)
                     /gene="tgt"
                     /locus_tag="P5179_08260"
     CDS             complement(3730..4875)
                     /gene="tgt"
                     /locus_tag="P5179_08260"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRS95249.1"
                     /translation="MHTLSFELSATDKNARAGIVTTRRGAFPTPMFMPVGTLGSVKTL
                     SQDDLRAAGAQIILGNTYHLLVRSDPSRIHRMGGLHGFMNWDHSILTDSGGFQVFSLG
                     DLNRVTEEGVVFRSHFDGSMYTLTPERSVEIQHQLGADIIMAFDECAPYPAPLAQVIS
                     AADRSFRWAVRSLAAHRKLSEGYRDTAPPALFGIAQGGTSLWLRREQARLLSRLDFPG
                     YAIGGLSVGEPKELMWAVLEEIVPLLPADKPRYLMGVGYPEDLVEAVARGVDMFDCVI
                     PTRNGRKGTVFTNTGRLILKSARFAEDTAPIEEDCPCEACRQYSRAYIRHLIRCDEIL
                     GMRLCTLHNVTFYLRLVRRMREEVVAGTFDQWRREFLTKYRLEEGNQ"
     gene            complement(5101..5316)
                     /locus_tag="P5179_08265"
     CDS             complement(5101..5316)
                     /locus_tag="P5179_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRS95250.1"
                     /translation="MKKLYRSTTDRKIAGICGGLAEMYDVDPLAVRLIAIALLLVTGL
                     FPLTVVYLLAWWLVPRRESSFPQAPQA"
     gene            complement(5405..5818)
                     /locus_tag="P5179_08270"
     CDS             complement(5405..5818)
                     /locus_tag="P5179_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95251.1"
                     /translation="MQDSLQIIMTSLEAMDTWKILVIGVLFLWAVKIVLGIFATGFTY
                     FALPWRRFIKGGSSGLTVDLPGNPAERMPVSVRILGGAITLLFVGVAILLCAALPLAS
                     PWVAVGIILAALGAGLLVFYGLVQREEKRAAKENR"
     gene            complement(5830..6102)
                     /locus_tag="P5179_08275"
                     /pseudo
     CDS             complement(5830..6102)
                     /locus_tag="P5179_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            complement(6234..6563)
                     /locus_tag="P5179_08280"
     CDS             complement(6234..6563)
                     /locus_tag="P5179_08280"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRS95252.1"
                     /translation="MSDNRAVDYRFSTGLKRAVYEVAPEVALFLYGSAARGTREPDSD
                     YDVLVLSEHALSDEEEGAIRNAVYDFELENEIVVSLVFFARGEWDSPLMSATPFHANV
                     TREGIQL"
     gene            complement(6565..6960)
                     /locus_tag="P5179_08285"
     CDS             complement(6565..6960)
                     /locus_tag="P5179_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95253.1"
                     /translation="MPYIDSIMGTNRGRLAVLFAGCMLVSLFIAAIALLAAPVAKRVF
                     AGVMCIIGKITKQEMPRDIQLSGRIVGTAALFLSAVACTIIIRLATVPRDGWHTIGVF
                     LLSVGSALIVSNSVITRLEKNESSTKGGN"
     gene            complement(7029..7166)
                     /locus_tag="P5179_08290"
     CDS             complement(7029..7166)
                     /locus_tag="P5179_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95254.1"
                     /translation="MAQRALGTTAISLDGGGMVKELGRVCRITAGSRSVPARGERTRE
                     E"
BASE COUNT         1427 a         2280 c         2085 g         1440 t
ORIGIN      
        1 aacccgaatg ctttcccgat ttttgcgacc actttcgcgt ccgcaaagtt gctcgtcggc
       61 tgaaccacca cactcaacac cttgttggca actgcggtgt caatcgtgat gatggtgtac
      121 ttaccgtctg cgatcttcgc gaggtacgct gccagggcag cgtcccgttc aacgtagtgg
      181 tggttgccgg ggcttccgaa cacgccaatg acaccgatgg tagtcacggt gtcctgctca
      241 agcaccttgt ccagcgcgtt gccgaacttg tcccggccgc accccgccgc ggcaaggcag
      301 atcatcccgc agatacaggg caacagcgca cgtctcaaaa gttccatgcc gtctcaccaa
      361 gtcctttctg gaagaggcac ccgtgtgcaa gctctccacg cgtcgttgca tatcgtggcg
      421 cgactcccct cgcgcccgca actggatttc acacgtacac ggtactacaa aacgaaccga
      481 tacccaacat tggctctcag gaacgtaagc ccgcccctcg tttccgggcg tctgcagcgg
      541 cgtccgcgat ggcctctctg ctgagtcccg cggcgtcgta catcgcaaag atgttctcgc
      601 tcggagtttc gggagggatg gtgtgggacg cagcaaggat gtatcccccg ccgtcctccg
      661 tcatgatctc aataagcttc cgcgtttccc ggaacacctc atcggcggtt gcctgtggga
      721 gcagcccctg cgtatccacc ccccccatga aggtgagctt ccggccaaac tcccttttca
      781 gcgaagcggg gtccatcccg gggcagttgc attgtatggg gttcagtaca tcgagaccta
      841 tttcgattag atcaggtatg atatcccgta ctgctccgca cgagtggtag gccaccggga
      901 tgcctcccgt cttgccggcg gcgaatatct ctttcaggcg cggtttcacc agctcacgcc
      961 agagagacgg actcatgatc atcgcgagct gtccgcccac gtcgtcgccg gtccagagcc
     1021 agtccagccg aaaacggcgg catgcttcct ccgcaagatg gcgggaaaac gcaccggctt
     1081 cggcaagcat cgtgcgtgcg aggtcgggat cttccgcaag gtcgagagtt gcctgctcca
     1141 tccctcgcag gcggcagatc atctcaaaca ggcagggcga cacgtcacaa ccgatgaaat
     1201 accggtcgct gtaccgcagc aggggcacca tgttcgccat caacgaatcc acgcggtcgt
     1261 aaggatattg gtacgcaaga gcctcttcgc gtgatgcgtg ttccagcggg gaactgcgta
     1321 tctggttgaa gtgccccgat ttcacccatt cgatgcccca gtcatctcga tgcgtttccc
     1381 cgtcacgttc gtggacaatc ccctccatcg cgtggttatt gcccacccac gtctggcgca
     1441 catcatcccc catcaccgtg gaaagctcgc tcaccggtat gtcaagctcg gccgcgagac
     1501 gccgggcagt gacgggatga aaccacatga aaatggggat gcggtcggca ggaaggcggt
     1561 tcagcgccgc gtgaaccctc tgtctgggtg tcatctgaaa cgtccaggtc ctattcccgc
     1621 ctatgccggc ggaacaagaa tcaccatcaa gagcgcgcca acgaagagaa aaacggcaac
     1681 gccgagcgtc agggcaagaa tccagcgttc cgccttggta cccctcgcca tatcccctcc
     1741 ttattcgaaa cgctcccctt tttgcgggtg agcaccgcga acccaggccg cgccttccat
     1801 ggggcgcctg cctgcgggct gcacgatatc cagccggaga atcccatccc ctgttgcaac
     1861 gcgcaccccg tgcctgtcgt ccgcctgaag gatcgtgccg ggcgcttcgt cgccgggggc
     1921 aggagcttcc tccacagcag ctcgaagaat gccgaacggc tgccccttcc acaaggcgta
     1981 cgcgccgggg gaaggattca gcgcacgtac ccggttgcgg atcgcttccg cgcgttcatt
     2041 ccaccggatc ctgccgtcct ccttcgtgag tttcggcgcg gtggttgcct cggcatcatt
     2101 ctggggaatc attacggcgg cgccgcgcgc aaggtcgtcc actgcggccg cgagttcttc
     2161 tgcccctgtc acgctcagcc gcgccgcgag ctcgccacag gtttcgtcat caccgatggc
     2221 gatcggtcgc tggcgaagta tccgccccgt gtctaccgtc ggctcaagca ggaacaccgt
     2281 tacaccggtc tcgcgttcac cgttgatgag cgcccgttgg atcggagcgg caccccggta
     2341 tttcggaagc agggacgcgt ggaggttgat ggccccgagg cgcgggattc gcagcagttc
     2401 ttccggcaga acgcggaatg cgaccacaac aaaaaggtcc ggctggagtg cttcaaggcg
     2461 ggagtgtgtg tccgtgtctc tgagcgacgg cggttgaata acaggaagcc ccagccgttc
     2521 cgccgcccgt ttgacaggag gaggctggat tgcccgaccg cggcccgccg gtttgtccgg
     2581 tttcgtcacc acggcaacaa cgtggtgccc tcccgacgcc agtttctcca gcgacggcac
     2641 gctgaattcg ggtgttccca tgaacacgat gcgaaggggt ttcacgacaa cacacccggg
     2701 aagcagcgag ggggcgggca catctcagcc cgtcccccgc tcaccagcgc ctgaacgaac
     2761 cgttttgctt ctcacgatcg ctttccctgc gacttcgcca gcgccttgag ctgtttgcgc
     2821 aggagttgcc gccggacggg gctgatgcga tcgacaaaaa ggatgccgtc gagatggtcg
     2881 tactcgtgct gtatgatccg cgccactatg tcgtccgcat ccaggtgcgc cggttcgccg
     2941 tcaatcgtct gatagtccac ggaaacccgc tcggggcgca ccacgtcagc cctgatgtca
     3001 ggcacgctca ggcacccttc ctcgatgacg acgtgctctc ccgaccgcgt aaccacggga
     3061 ttgatcagcg caaaagcttt gcctttctga tctcccgcgg tggggtcgat cacgatcatg
     3121 cgaatcgaaa cccccacctg cggcgccgcc agtcctaccc ccggttccgc atacatggtt
     3181 tcgatcatgt cggttgccag cgtccgcagt tccggcgtga attcgcgtac cggttccgcg
     3241 acgcggcgga gaacagggtc accgtagatg cgaatcggga gtaaggccat gctggaaatg
     3301 tcctgaacgc tgatgcgatt ctactcggag gcggcggatt ccacgacaga ggaaatggcg
     3361 gagcgctgga cctccacctt cacgttttcg gcgatccgga gcacaacgac actgtccttc
     3421 acctgctgga tatccccgta gatgccgcca agagtaagta cgcgatcgcc cttcttcagc
     3481 gcctcgagca tcttttcttt ttccttctgc cgcttctgct gcgggcggat catgaagaag
     3541 tacagaacca cgaacatgag cagcagggga acaaacatac cccaggggct gccggcctgc
     3601 cctccggaag gctgggcacc catggcaaat gcgtttcctc cggtcagcat caatcctgcg
     3661 atggtcaatc gagcaacacg catgcagtgt ctccttggtt gtgcggcatc tttccgagta
     3721 tcaacaacgc tactggttcc cctcttcgag ccggtatttg gtaaggaatt cccgtcgcca
     3781 ctgatcaaac gttcccgcca cgacctcttc tctcatccgg cgaaccaggc ggagataaaa
     3841 tgtaacattg tggagggtac agagcctcat tccgaggatt tcgtcacacc gaatgagatg
     3901 tcggatgtag gcgcgcgagt attgccggca ggcctcgcag ggacagtcct cctctatcgg
     3961 ggcagtgtcc tccgcgaacc gcgcggattt tagaatgagc cgccctgtgt tcgtgaacac
     4021 ggtccctttc ctcccgttcc gcgtcggaat cacgcagtcg aacatgtcca cgccgcgggc
     4081 aacggcttcc acaaggtcct ccgggtatcc gacgcccatc aggtaccgcg gtttgtctgc
     4141 gggcagcagc ggtactatct cctccagcac cgcccacatg agctcctttg gttcaccgac
     4201 tgacaggccc ccgatggcat agccggggaa atcaagccgg gagagaaggc gggcctgttc
     4261 ccgtctcaac cagagggagg tgccaccctg cgcgatgccg aacagagccg gcggtgctgt
     4321 gtccctgtac ccctcgctga gctttcggtg cgccgccagc gaacggactg cccatcggaa
     4381 cgagcgatcg gcggcagaga tgacctgcgc aaggggagcc gggtacgggg cgcattcgtc
     4441 aaacgccatg atgatgtcgg caccgagctg gtgctggatc tccaccgagc gttccggtgt
     4501 cagggtatac attgagccgt caaagtggct ccgaaacacg acgccctctt ctgttacccg
     4561 gttcaggtca ccaagactga acacctggaa tccgccgctg tcggtaagga tgctgtggtc
     4621 ccagttcatg aaaccgtgca ggcctcccat gcgatggata cgggaaggat cggagcgcac
     4681 cagcaagtgg tatgtgtttc cgaggatgat ctgagctccg gcggcacgga gatcatcctg
     4741 cgagagcgtc ttgacacttc ccagcgtgcc gaccggcatg aacatcggcg tcgggaacgc
     4801 accgcgccgt gtcgtaacga tgcccgcgcg ggcatttttg tcggtggcag agagttcgaa
     4861 ggaaagtgtg tgcacgggta acgccggaat cggggcggat accacagagt cgcagcaatc
     4921 tggacagaag cagtgggaaa tatacgattc gcgaggtgtg ccggcacacg cggacgtcga
     4981 agaagccggc cgaagcggga ttgcccatcg caggaccgac gcccggtttc cggcacgaaa
     5041 ccaccgcctg aggggcactg ctcacgacag aaactgaacg cgtgactttg acagcggcgc
     5101 tcaggcctgt ggcgcctgcg ggaacgagga ttcgcgccgg ggcacaagcc accacgcaag
     5161 gaggtacacc actgtgagcg ggaagaggcc ggtgacgagg agcagtgcta tcgcaatcag
     5221 tctgacggcc aacgggtcca cgtcgtacat ctctgccaat ccgccgcaga tgccagcgat
     5281 tttcctgtca gtggtcgatc ggtacagctt tttcatcctg tttgttcctc atgcatgtgc
     5341 gtggagatgc gcgcgctgta ttcccgcgga aacggcgtgg tcggccttcc ggccccgcga
     5401 tttcctaacg gttttctttc gccgcgcgct tttcttccct ttgcaccagc ccgtagaaca
     5461 ccagcagacc tgctcccagc gccgcgagga tgattccgac ggcgacccac ggcgatgcca
     5521 gcggcaaggc agcgcacagg aggattgcaa ccccgacgaa gagcagggtt atcgcgcctc
     5581 ccagaatacg gacggaaacc ggcattcgtt cagcagggtt tcccgggagg tccaccgtga
     5641 ggccgctgct accccctttg atgaatctgc gccacggcag cgcgaaatac gtgaatcccg
     5701 tcgcgaatat cccgagtacg atcttcactg cccagagaaa cagtactccg atgacgagaa
     5761 tcttccatgt gtccatggct tcgagggagg tcatgatgat ttgaagggaa tcctgcacgg
     5821 tacgaactcc tattcgttgc tgcgtattcg actttcaacg actgcactga tagtttccac
     5881 gaagtatgcg gccgacttct gccattcccg aacgtcgtcc ggtgggaatg tcgtcagatc
     5941 ggcgtaatcc cctttctggc gcaaatcgaa aagccggtgg tagaacctac ccgcatcctt
     6001 tgggacgcgc ccgctcttaa cccagtttgc gtcaaacaga gcccgaacgc cgctgtgttt
     6061 tgcagatgac tggccctcag tcagaaggag gccactcaca gcatagaaac aagcgtatta
     6121 gagccggtta accgccgcat tcaagtggtc gttgtccagt aacaactcgg cttcgccaag
     6181 ggcctctttc gcgcgttcga ggcggtatcg gacgagcgtg acctgctccg gggtcatagc
     6241 tgaatccctt cccgcgtgac gttggcatgg aacggcgtgg ccgacatcag aggagaatcc
     6301 cactcgccgc gcgcaaaaaa gacgagggaa accacgatct cgttctccag ctcgaagtca
     6361 tacaccgcgt tccggatcgc gccctcctct tcgtcggaga gcgcgtgctc cgacagaacc
     6421 agcacgtcgt aatcagagtc gggttcgcgg gtcccgcggg cggcgctacc gtaaaggaac
     6481 agcgccactt ccggagccac ttcgtaaact gcgcgcttga ggcccgtaga gaacctgtaa
     6541 tctaccgcgc ggttgtctga cacctcagtt tcctcctttc gtggaggatt cgttcttttc
     6601 cagtctggtg atgacggaat tggagacgat gagcgcagat cccacgctta gcaggaaaac
     6661 gccaatcgtg tgccatccat cgcgaggtac ggtcgctaag cgtatgatga tcgtgcacgc
     6721 cacggcgctg agaaaaaggg cggccgttcc aacgatacgc cctgaaagct ggatgtcccg
     6781 tggcatttcc tgcttcgtga tttttccgat gatacacatg acgccggcga aaacgcgttt
     6841 tgccacggga gccgccaaaa gggcgatcgc ggcgatgaac aggcttacga gcatgcaacc
     6901 tgcaaacagc acggcgagtc tgcctctgtt cgtccccatg atgctgtcaa tatagggcat
     6961 cacccgtttc tctccacaaa aaccatgttc tcgccagttt cctttgcgtg cctcaccgcc
     7021 atctcgagct attcctcgcg ggtgcgctcg ccgcgcgccg gcacagaacg tgatccggca
     7081 gtgatacggc agacacgtcc cagttctttc accattccgc caccgtccag ggagattgcg
     7141 gtagtgccca gcgcacgttg cgccatctgc tcctcagtct gcttcttcag cactgcgtgg
     7201 tggcggagtc attttctcaa acttcgcgac ga
//
LOCUS       DAOTNG010000149         7180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9095_length_7180_cov_4.762807, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000149 DAOTNG010000000
VERSION     DAOTNG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9095_length_7180_cov_4.762807"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /gene="hisG"
                     /locus_tag="P5179_08295"
     CDS             complement(<1..554)
                     /gene="hisG"
                     /locus_tag="P5179_08295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRS95255.1"
                     /translation="MAQFVTFGLPKGSLQEATLDLMKRAGFEFTVSSRSYFPTCDDPE
                     VKAVMFRAQEIAKYTERGVMDVGLTGLDWILDSGADVVEVADLIYSKVTTQKARWVLA
                     VPENSPIKEAKDLQGKRIATELVHVVEAYLQRHGVNAEVEYSWGATEVKVPDIVDAIV
                     DLTETGSSLRAHKLRVVDTILETNT"
     gene            733..1863
                     /locus_tag="P5179_08300"
     CDS             733..1863
                     /locus_tag="P5179_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95256.1"
                     /translation="MQSERVQMTDPKTGIQVIQLTSYPTPSIPLNYDWPSVTPDNRRV
                     VFLAQRSAEREAPWDIYRCDTDGLDLVRLTKKDASAGYPDVTLSLDGKSAYVIWREEK
                     ILRKMDMETGAMEDLLSVADHCPPGLAVLAVRYGSLGSRLYLDLRDCMAGEGRMLRVD
                     LATGAVTANDTNMTIAAVDQATGRLIVIKNFMKLGTKMGADGARVFHNANPEPMRYYS
                     VKEDGTDEQYIADVDMYAHSTILGKTSQIQGPSQPPHRCIWISEAGKVPYKLVEGPYF
                     WHAGASYDAEWILSDTNWPDQGLQLVHVPSRNFRTVCYSGATLGHSQWGHSHPGLSQD
                     GRIGVFASDRTGITQVYVALITDEFRESVVAGQLDNPKDKWI"
     gene            1934..3061
                     /locus_tag="P5179_08305"
     CDS             1934..3061
                     /locus_tag="P5179_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95257.1"
                     /translation="MSERVQMFDPKTGIQVVQLMSYPTPSIPLMYDWPSVTPDNRRVV
                     FLVQRAASREAPWDIYRCDTDGLDPRQLTRKEDTAGYPNVTLSLDGKSAYVLWRDEKI
                     LRKLDIETGAMEDLIAVGNHCAPGMIVCGVRYGSVGSRLYLDLRDYAAGEGRMIRIDL
                     ATGAVTANDTNMTIAAVDQATGRLIVTKNFMKLGTRITENGNRVFHNVNPEPMRYYSV
                     NEDGSDEQYICDVGMFAHSTILGNTSKIQGPSQPPHRCIWIAEAGTEPYKLVEGPYFW
                     HAGASYDAEWILSDTNWPDQGLQLVHVPSRHFRTLCHAGATQGHAQWGHPHPGLSQDG
                     RIGVFTSDRTGMAQVYVAHITEEFRESVIAGQLDNPKDKWI"
     gene            complement(3140..4141)
                     /gene="pta"
                     /locus_tag="P5179_08310"
     CDS             complement(3140..4141)
                     /gene="pta"
                     /locus_tag="P5179_08310"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRS95258.1"
                     /translation="MSSTVIQQIRERAAARKGRIILSEGNELRAIQAARKLLDDGICE
                     VTLVGAPAEIEKVAKEGGVSVSGITMVDPSEHPMREQFAAELYEKRKAKGLTQEQAEE
                     LSRRQIYCAAMMVSHGEGTGTVGGAVHATAEIVRAGLWCIGMAKDVSVVSGAFLMVVP
                     DFLGTGKDKTLYYADSGVVPDPNSDQLASIAIASAATFRALTGEEPRIAMLSFSTKGS
                     ATHPDVDKVIVATKKVQELRPDLLIDGELQADAALVPGVAKAKAPGSSVAGTANVLIF
                     PDLGAGNIGYKLTQRLAHATALGPILQGLAKPAHDLSRGCSAEDIVDMAAIAICMAE"
     gene            complement(4191..4676)
                     /gene="coaD"
                     /locus_tag="P5179_08315"
     CDS             complement(4191..4676)
                     /gene="coaD"
                     /locus_tag="P5179_08315"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRS95259.1"
                     /translation="MRRALYPGTFDPITLGHLDIIERSLRIFDEVVVSVADTRPGVVF
                     PVEDRVRLVEAATKHLDRVRVVSFRALLVHHWQSLGKPTVVRGLRAVQDFEAEFAMAL
                     GNRRLADDFEIIFLMPRNDFVFLSASIVREVARWGGDVSSMVSPPVAEYLATRYGPPA
                     K"
     gene            complement(4702..>5015)
                     /locus_tag="P5179_08320"
     CDS             complement(4702..>5015)
                     /locus_tag="P5179_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95260.1"
                     /translation="MRRKAMPLAVRLVIAVIAFSAAAIPPLIRYSAAEEAEECARRRE
                     AVRTAASRLPDERWWNERGCPRDGEDYRFAVGQDSTVEVVCPKGHGIQKLPVHRWFSP
                     F"
     assembly_gap    5016..5115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5116..5495)
                     /locus_tag="P5179_08325"
     CDS             complement(<5116..5495)
                     /locus_tag="P5179_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRS95261.1"
                     /translation="MCANERRFAFSRRELAALAALTVSLAVGCFLVWLIPEPSDLRVV
                     RSPELASLAREVAPSTGSHLININTASFDELVSLPGIGPKKAQAILDYRHANGRFPDV
                     GAIERVKGIGPATVARLRPLITVQ"
     gene            complement(5538..6446)
                     /locus_tag="P5179_08330"
     CDS             complement(5538..6446)
                     /locus_tag="P5179_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRS95262.1"
                     /translation="MKKLSVVAPICGLFAACSHSANLSRALGSVNERRAFGYLTAQTA
                     FGPRNPGSRGHAACRDYLIRQLNALTDTVELQHFQPIDSRGNRTPPMTNIIGRIAPHQ
                     NRRYLLCAHWDTRPRADRDPDPRRRDLPIPGANDGASGVAVLLEIATVLKRVSPPVGI
                     DIVFFDGEDYGEEGRLEDYFLGSREFARRWRGQKHEMAILVDMVGDRNLQLPVEAFSW
                     QAAPEVVNRVWGAAERLGERAFIRSMGVEIQDDHVPLIEAGWRAIDVIDFSYPYWHTH
                     GDTPDKCSPQSLGSVARVLLAAIIER"
     gene            6537..7022
                     /locus_tag="P5179_08335"
     CDS             6537..7022
                     /locus_tag="P5179_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95263.1"
                     /translation="MSPRAIVALAVLGFSFLAPSFVSAATRSGGWDKSVYFGPFAVGY
                     ATTKPFSGGGTPQLRWGWKVEGGWTGGGFGGASTYNDALIVSPQGLIRLAIPLEGTMV
                     LYPQLGLGVAARFDDGSGTHSGAWVGPIGSFGIGLQFGKWGALIQGPGLPGIGVTYHF
                     Q"
BASE COUNT         1420 a         2266 c         2071 g         1323 t
ORIGIN      
        1 gtgttcgtct caagaatcgt atccaccacc cgcagcttgt gcgcgcggag gctgctgccc
       61 gtttcggtca ggtcaacaat cgcgtccact atgtcgggca ccttgacctc ggtcgccccc
      121 cacgagtact ccacctccgc gttgacgccg tgccgttgca ggtacgcctc caccacgtgc
      181 accagctccg tcgcgatgcg cttgccctga aggtctttcg cctccttgat gggcgagttc
      241 tccggcaccg ccagcaccca gcgcgccttc tgcgtcgtga ctttgctgta aatcaggtcc
      301 gccacctcca ccacgtccgc gccgctgtcg agtatccagt cgaggcccgt cagcccgaca
      361 tccatgacgc cgcgctccgt gtatttcgca atctcctggg cgcggaacat gacggccttc
      421 acttccgggt cgtcacacgt ggggaaatac gagcggctgc ttacggtaaa ctcaaacccc
      481 gcacgtttca tgaggtcgag cgttgcttcc tgaaggctgc ccttggggag cccgaacgtg
      541 acgaattgtg ccattttttg ttcctgttca gatggtgaaa aagcctcaga gaacgccgga
      601 ttgcgccggt tgcgacgtac aaaagatagg gaaacgccaa accgggtgcg ttcccacttc
      661 ctcactgcgg tgcgtgattc atacgtgcgt ttcccgcgaa aaaggcacca aaccgattcc
      721 cgaggggata ccatgcaaag tgagcgcgtg cagatgaccg acccgaaaac cgggatacag
      781 gtcatccagc ttacgagtta ccccacgccc agcataccgc tcaattatga ctggccgagc
      841 gtcacaccgg ataaccgccg tgtcgtgttc cttgcccagc gcagtgcgga gcgcgaagcg
      901 ccgtgggaca tctaccgttg tgatacggac ggattggacc ttgtccggct cacgaagaag
      961 gacgcgagcg cagggtaccc ggacgtcacc ctcagcctcg atggaaagag cgcgtacgtc
     1021 atctggcggg aagagaagat actccggaag atggacatgg aaaccggcgc gatggaggac
     1081 ctcctttccg tcgccgacca ctgcccgccc ggactggcgg tactcgccgt acgatacggt
     1141 tcgctgggtt cgcggttgta cctggacttg cgtgattgca tggccggcga aggcaggatg
     1201 ctacgcgtgg accttgccac gggtgcggtc acggcaaacg acacgaatat gaccattgcg
     1261 gcggtggatc aggccaccgg ccggctcatc gtcataaaga acttcatgaa actcggcacg
     1321 aagatgggcg cggacggagc acgggtgttt cacaacgcca acccggagcc catgagatac
     1381 tacagcgtca aagaagacgg aacggacgag caatacatcg ccgacgtcga tatgtacgcg
     1441 cactctacga ttctcggcaa aacgagtcag atccaaggcc cgagtcagcc accgcaccgt
     1501 tgcatctgga tttccgaagc cggcaaggtg ccatacaaac ttgtcgaggg accctatttc
     1561 tggcacgcag gagcctccta cgacgccgaa tggatcctct ccgacacgaa ctggccggat
     1621 caggggctcc agctcgtgca cgtccccagc cggaacttcc gcacggtctg ttattctggt
     1681 gcgacgctgg gacattctca gtgggggcac tcgcatcccg gtctcagcca ggatggccgt
     1741 atcggagtgt tcgcctccga ccggaccggg ataacacagg tctatgtcgc cctgataaca
     1801 gacgagtttc gcgagagcgt tgtcgcaggg caacttgaca acccgaaaga caaatggata
     1861 tgagcgcacg cgcgaaacgc ctgctgcccc gaaagggaat ggcgcaggtc cgcacacacg
     1921 aaggagtctc tcgatgagtg aacgtgttca gatgttcgac ccgaaaaccg gaattcaagt
     1981 tgtccagttg atgagttatc ccacacccag cattccgctc atgtacgact ggcccagcgt
     2041 cacgccggac aacaggcgcg tcgtattcct tgttcaacgc gccgcgtcgc gcgaggcgcc
     2101 gtgggatatc tatcggtgcg acaccgatgg gttggacccc cgtcagctca cacggaaaga
     2161 agacaccgcg gggtatccca atgtgacgct cagcctcgac ggcaagagcg catacgtgct
     2221 ctggcgcgac gagaaaatcc tgcgaaaact cgatatcgaa accggagcga tggaagacct
     2281 catcgccgtc ggcaaccact gcgcgcccgg catgatcgtc tgcggcgtcc ggtacggatc
     2341 agtcggttcc cgcctctacc tcgacctgcg ggattacgcg gcaggggaag gcaggatgat
     2401 acggatagac cttgccacgg gtgccgtcac ggcaaatgac acgaacatga cgattgcggc
     2461 ggtagatcag gccaccggca ggctcatcgt cacgaagaac ttcatgaaac tcggcacgag
     2521 gatcaccgag aacggaaacc gtgtgttcca caatgtcaac ccggagccga tgcggtacta
     2581 cagcgtgaac gaggacggca gcgacgagca gtacatctgt gacgtcggga tgttcgccca
     2641 ctcaacgatt ctcggcaaca cgagcaaaat ccaggggccc agccagcctc ctcatcgttg
     2701 catctggatt gccgaagcgg gcaccgaacc gtacaagctc gtggaaggcc cctatttctg
     2761 gcacgcggga gcctcgtacg acgccgaatg gatcctctcc gacacgaact ggccggatca
     2821 ggggctccag ctcgtgcacg tccccagccg gcatttccgc acactctgcc atgccggggc
     2881 cactcagggc catgctcagt gggggcatcc gcaccccggg ctgagtcagg acggcagaat
     2941 cggggtgttc acctccgaca gaacgggaat ggcgcaggtc tacgtcgccc acatcacgga
     3001 ggagttccga gaaagcgtca ttgccgggca actcgacaat ccgaaagaca aatggatctg
     3061 agccctgcct gaggcggaaa tcgaacgggg aggcgcgttt cggcgcttcc ccgtttttcg
     3121 tggtggctcg gctgcggagc tattcggcca tgcagatcgc gatcgccgcc atatccacga
     3181 tgtcctcggc gctgcaaccg cgggaaagat cgtgcgcggg cttcgccaac ccctgaagga
     3241 tcggcccgag ggccgtggcg tgggcaagcc gctgggtgag tttgtaccca atgtttcccg
     3301 cgccgaggtc cgggaaaatg aggacgttcg cagttccggc gaccgaactg cccggcgcct
     3361 tcgccttcgc cacacccggg acgagcgccg cgtcagcctg cagttcgccg tcaatgagga
     3421 ggtccggccg caattcctga accttcttcg tcgcaacaat caccttgtca acgtcggggt
     3481 gcgtcgccga ccccttggtg gagaagctca gcattgcaat gcgcggctcc tcaccggtaa
     3541 gcgcacggaa cgtggcggcg ctggcaatgg caatggaagc gagctggtcg ctgttcgggt
     3601 caggcactac gccggaatcg gcgtaataca gcgttttgtc cttgcccgtt ccgaggaaat
     3661 cgggcactac catgaggaac gcacccgaga cgacgctcac atccttcgcc atcccgatgc
     3721 accagagccc ggcgcggacg atttccgcgg tcgcgtgcac cgcgccgcca acggtacccg
     3781 tgccctcgcc gtggctcacc atcatcgccg cgcagtaaat ctgccgccgt gaaagttcct
     3841 ccgcctgttc ctgcgtcagg ccctttgcct tccgtttttc atacagctcc gcggcaaatt
     3901 gctcccgcat ggggtgttcc gacggatcca ccatcgtgat gccggataca ctgacacccc
     3961 cctcttttgc cactttctcg atctccgccg gcgcacccac cagtgtcacc tcgcagatcc
     4021 cgtcgtcgag gagtttccgc gccgcctgaa tggctcgaag ttcgttcccc tcggacagga
     4081 tgatacgtcc cttgcgtgcg gctgcacgtt cgcggatttg ttgaatgacg gtactgctca
     4141 tcagggttgc ctccccacat ttgatgtcca aggccacagc cgggcaatcg tcatttcgcc
     4201 ggcgggccgt atctggttgc gaggtactcc gcgacagggg gagaaaccat gctggagacg
     4261 tccccacccc accgcgcaac ctctctgact atcgacgcgc tgagaaacac gaagtcattt
     4321 ctcggcatca aaaatatgat ctcgaaatca tcggcgagcc ttctgttgcc gagggccatt
     4381 gcgaactctg cttcgaaatc ctgcactgcg cgcaatccgc gcaccactgt cggcttgccg
     4441 aggctttgcc agtggtgaac gaggagcgcg cggaacgaga ccacgcgaac tcgatcgaga
     4501 tgcttcgtcg ccgcttctac cagcctgaca cgatcttcca cgggaaacac cacgccgggc
     4561 cgggtgtccg caacagatac cactacttcg tcgaaaatcc gcaggcttcg ctcaatgatg
     4621 tcgagatgcc ccagcgtgat gggatcgaag gttcccggat agagcgcgcg acgcatgtcg
     4681 gatcgtcctt tcaaccgtcg ttcagaacgg ggaaaaccat cggtgcacgg gaagcttctg
     4741 aatcccatgc cctttgggac acactacttc aaccgtgctg tcctgcccca cggcaaagcg
     4801 gtagtcctcg ccatcacgcg ggcacccgcg ttcgttccac cagcgttcgt ccggcaaccg
     4861 cgatgccgcg gttcttactg cctcccgccg acgcgcgcat tcctccgcct cttccgccgc
     4921 gctgtatctg atgaggggcg ggatggcagc cgcgctgaac gctatcacag ctatcaccag
     4981 ccggaccgcc agcggcatgg ctttgcgtct catctnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnntcctg aacagtgatg agcggccgaa ggcgtgccac cgtcgcaggc
     5161 ccaatgcctt tcaccctctc aatcgcaccc acatcaggaa agcgtccgtt cgcatgccga
     5221 taatccagta ttgcctgcgc cttcttcggc ccgatcccgg ggagggaaac cagttcgtca
     5281 aacgacgcgg tgttgatgtt gatcaaatgg gacccggttg acggcgcaac ctcgcgagcc
     5341 agactcgcca gttcaggcga tcgaacgact cgcaggtcgg atggctccgg aatgagccag
     5401 acaaggaaac aaccgaccgc gaggctcacc gtcaacgcgg cgagggcggc aagctcccga
     5461 cgcgagaagg caaagcgcct ttcgttcgcg cacatgggac tttgattgga gccggagatc
     5521 tccattgtca gccacggcta ccgttcgatg atcgccgcca gaagaactcg cgcgacactc
     5581 ccgaggctct gtggcgagca cttgtcgggg gtatcgccat gggtatgcca gtacggatag
     5641 gaaaagtcga taacatcgat ggcccgccac ccggcttcaa tcagcggaac gtgatcatcc
     5701 tgaatctcca cgcccatgga gcggatgaac gcacgttcgc ccagcctttc agcagccccc
     5761 cacaccctgt tgacaacttc cggagctgct tgccacgaga acgcctcgac aggcaactgg
     5821 agattccggt cacctaccat gtccaccaga atggccattt cgtgcttctg gccacgccag
     5881 cgccgcgcaa actccctcga cccgaggaag tagtcctcca ggcgtccttc ctccccgtag
     5941 tcctcgccgt cgaaaaagac aatatctatg cccacagggg gggaaactcg tttgagaacg
     6001 gtggcaattt ccaggagaac ggccacccct gaagcaccgt cattagctcc agggatcggc
     6061 aggtcgcgcc gtcgcggatc gggatcacga tccgcccgcg gcctcgtatc ccaatgtgca
     6121 cacaaaagat agcgcctgtt ctggtggggc gcaatccgcc caataatgtt cgtcatcggc
     6181 ggcgtgcgat tgccgcgcga gtcaatgggc tgaaaatgtt gcaattctac cgtatcggtg
     6241 agcgcgttca actgacggat gaggtagtca cggcaggccg cgtggccccg gcttccaggg
     6301 ttcctcgggc cgaacgcagt ctgggcggtg agatacccga acgcccgtcg ctcattgacg
     6361 gaacccagcg cccggcttaa gtttgcggag tgggaacacg ccgcgaacag accgcatatg
     6421 ggagcgacca cactcagctt tttcaaggtg tcttccccgc gttcgttcaa gccatggtga
     6481 aagatggcac gaggtcacac cgagacgcaa gtctcgaaca atattggagg tacgctatgt
     6541 caccacgcgc tattgtcgcg ctggcggtgc tgggcttctc attcctcgcg ccttcgtttg
     6601 tctcagccgc cacgagatcc gggggatggg acaaaagcgt gtacttcggc ccgtttgcag
     6661 tggggtatgc caccacaaag cctttctccg gcggcggcac acctcagttg cgctggggct
     6721 ggaaagttga gggcggctgg accggcggcg ggttcggcgg cgcaagcacc tacaacgacg
     6781 ctctcatcgt gtcccctcaa ggcctgatcc gccttgccat tccgctcgaa gggacgatgg
     6841 tgctctaccc tcagctcggg cttggcgtgg cggcgcggtt cgatgacgga tccggcacgc
     6901 acagtggcgc ctgggtcggt cctatcggct ccttcggcat cgggctccag tttggcaaat
     6961 ggggcgcgct gattcaggga cccgggcttc ccggtattgg tgtcacgtac cacttccagt
     7021 aggccgtaat cgcccatgct cgcgtcgccc gcgttgcttg cctttattgt cggcctgctg
     7081 atcggcatcg ccgcggctct gctcgtcgcg cggaaccgca tcgaacgcgc cctgacacag
     7141 gcggccaccg aagcggcgac ggcgcgcgcc gtgctggaag
//
LOCUS       DAOTNG010000150         7159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9126_length_7159_cov_5.237472, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000150 DAOTNG010000000
VERSION     DAOTNG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9126_length_7159_cov_5.237472"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="P5179_08340"
     CDS             complement(<1..866)
                     /locus_tag="P5179_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95264.1"
                     /translation="MFSVGLAPLKRVLAAIAVACSTGVLGCAAFAPRPAHVTAEHLDP
                     LQAFTVLKESPRTYKVRVGSLRRYRVSAPFVGQEPVQVYDLPAELGELWDEATSEALY
                     GNPHRSAQMLEAIVNAEPACAECRHEYARVLINLANKEKLTKERAHELRYRALDEMTI
                     AGVLDRECPSGAGAFYPPTSAGRTDIPLLPRTRQAGELFAAGNDAFNSGEFTQARSLY
                     GVVVQREPTFGRGYVGLGDVFVELGAYQEAAEYFTRALSVDSTDFLAWRSLAECAFAS
                     GNAELAKEAAVRA"
     gene            complement(839..2011)
                     /locus_tag="P5179_08345"
     CDS             complement(839..2011)
                     /locus_tag="P5179_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRS95265.1"
                     /translation="MRDSRRTLRSGIVLPWTIVLGFLAVAMCLATGIRATDNGREGTR
                     PDATNSVSAQRLSRVPLQSDSRSDSFRVRFRTEFAKSLKNSGFAGDVKPRNRGATGDV
                     LVWDVRVPVGYSVLRATAEVISVVHSFGGNVYRAEHEYADPLKVKLAVGAAHAVTDSI
                     LLATDRSIASRGLLAIVIDDMGYRSMAESGRFADLPFPVNLAFLPAGENTLELAKRAR
                     AARKTILLHLPMEPENGSEPLEPNTIMTAMGDEEIRELTRRHLESVPGAAGVNNHMGS
                     RATRDERVMRAVLAEVRDRNLFFLDSYTSPGSTAAATARSLGIPTLERDVFLDNSSDI
                     QTIQRELWAAADKASRSGRAVAIGHDRPETYEALRVTIPAIQRSGVRVCFLWDLLR"
     gene            2165..2241
                     /locus_tag="P5179_08350"
     tRNA            2165..2241
                     /locus_tag="P5179_08350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Val,seq:gac)
     gene            2478..2687
                     /locus_tag="P5179_08355"
                     /pseudo
     CDS             2478..2687
                     /locus_tag="P5179_08355"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2835..3272
                     /locus_tag="P5179_08360"
     CDS             2835..3272
                     /locus_tag="P5179_08360"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRS95266.1"
                     /translation="MERQVRVLFLGTGNACRSQMAEAWAKTLKSHEMEVFSAGIEKQG
                     LSRRAVRVMAEAGVDISAYRSKTVGELPNRDFDFVITLSNAAHVNCPRFPGNPKINCN
                     VFDDPPALAEGSRDDAEALSHYRRVRDEIRAFVETLPEALQTL"
     gene            3491..4759
                     /gene="murA"
                     /locus_tag="P5179_08365"
     CDS             3491..4759
                     /gene="murA"
                     /locus_tag="P5179_08365"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRS95267.1"
                     /translation="MDAFRIQGGTRLAGSVEIEGMKNAILPMMAASILATEGTLVLEN
                     VPQLRDVTVMLRILETLGVKGGYDPQKRTLSLDATGVIGDTAPYDLVRQMRASFLVSG
                     ALVARLGRCRVSMPGGCAIGSRPVSDQLRAFKALGGVVNEIKGYVEVEARNRSGRLVT
                     LDYPAATGTENIVLLACLTEGTTTIENAACDPEVVDFGECLVKMGAQITGLGTNTVRV
                     TGVKALRGTRHRVVPDRIDAATFAVGAAMTRGDVFLANARSDHFEIVLTKLEEAGAIV
                     TRLDTGVRVQGPDRLKSVNVRTLPYPAFPTDVQAPMMAAMATAEGASLIWEQVYDNRF
                     TQGPELRRMGANITIAGDKAVVVGVPELTGAPVMASDIRAGGSLVLAGLVAKGETTVS
                     RVYHIDRGYDHLETRLQKLGAVIERYNEPA"
     gene            4756..5772
                     /gene="rsgA"
                     /locus_tag="P5179_08370"
     CDS             4756..5772
                     /gene="rsgA"
                     /locus_tag="P5179_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRS95268.1"
                     /translation="MIFAQSIVATSMCEASFVGTVIRKHRNFYVVERDATEVLCSLSS
                     KLRKNLEYPEADISSRRRRVQAVHKVGFVDPVVVGDDVRVEPGAGIGLIVERLERRSV
                     LSREAPSRTHLQQVIAANVDQVLAVVSPQSPPFRPDLLDRLLVGCEHHNLKGIVCLTK
                     IDLGVPGEVERVIETYPGIGYPLVRTSAVTGDGLQDLEQLLAGRNTVAVGLSGTGKSS
                     LVNALVPGANLPVGHVNRKTGLGTHRTTTISLHRLASGGHLVDAPGLRELGLWDCSAD
                     QLPDLFPEFRNLVDACRFRGCAHRDEPGCAVKTAVEENAIAHHRYRNYLSIRAELERR
                     EQQG"
     gene            complement(5773..6597)
                     /locus_tag="P5179_08375"
     CDS             complement(5773..6597)
                     /locus_tag="P5179_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS95269.1"
                     /translation="MPVSVAGPGVILNFRVSPCTKEPALSHSDIITVLAAFAIGLSKT
                     GFGGGLGAIVSPMFVIALPPREGIGLMLPLLIVGDMLTLTFYWRGWDRSNLVALFPGV
                     VVGIGLGMILLGHIPDNEFRVVIGLLAMVFGSGQALRQWLVPHAEAFTGNRFLGALAG
                     FVTGTVSAIAHQGGLVTTLYLLPQRLTNQAFVATSGIVFTLTNLTKLPAYVFAGLVTW
                     PIIVKAATLSPAVMLGALLGVRLNRQVPQKHFAWIVLFFVLVTGVKLVWDYFMSTG"
     gene            6625..>7159
                     /locus_tag="P5179_08380"
     CDS             6625..>7159
                     /locus_tag="P5179_08380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS95270.1"
                     /translation="MIEITPRDAARARGDTKRRYVTAMFDRIAPRYDAMNIVISLGQT
                     SIWRRRALKGISLPHRAVILDVGCGTGSVIRLLKRRFRSARYLGIDLSSRMLEKASLR
                     EPESDFCVGDAGSIPCRTETCDLITTFFTTRNFSDLPAAVREMMRVLKPGGRLVVLDS
                     FPASGSPLWRVLKKGWMY"
BASE COUNT         1524 a         2063 c         2170 g         1402 t
ORIGIN      
        1 gcccttacgg cagcctcttt cgcaagttcc gcatttcctg acgcaaatgc gcactcggca
       61 agtgagcgcc acgctaagaa atccgtgctg tccacgctca gtgcccgggt gaaatactcg
      121 gcggcctcct gataggcgcc cagttccacg aaaacatccc ccaatccgac gtagccacgt
      181 ccgaatgtcg gttcgcgctg cacaacgacc ccgtaaagcg aacgcgcttg cgtgaactcg
      241 ccgctgttga acgcatcatt tcccgcggcg aacaactccc ctgcctgtct tgtgcgcggc
      301 aaaaggggga tgtcagttcg tccggcggac gtcggagggt aaaacgcgcc cgcgcctgag
      361 gggcactcgc gatcgagaac accggcgatc gtcatttcgt ccagtgcgcg ataccgcagc
      421 tcgtgtgcgc gttcctttgt gagtttttct ttgttcgcca ggtttatcag aacacgggca
      481 tactcatgcc tgcattccgc gcacgcaggt tccgcattta caatcgcttc aagcatctgc
      541 gcgctacggt gcggatttcc atacagcgcc tctgacgtcg cctcgtccca cagttcccca
      601 agttccgccg gcaggtcgta gacctgaacg ggctcctgtc caacaaatgg cgcggacacg
      661 cgatacctgc gaagactccc gactcgcacc ttgtacgtgc gtggtgactc tttgagcacc
      721 gtaaaggcct gtaacgggtc gaggtgttcc gcggttacgt gagccgggcg aggcgcgaac
      781 gccgcacagc cgagcacacc ggttgaacag gcaactgcaa tcgccgcaag gacacgcttc
      841 aacggagcaa gtcccacaga aaacacacgc gaacccctga cctctgaatt gccggaatcg
      901 tcacacgcaa cgcctcgtac gtttcggggc gatcgtggcc tatcgctacg gcgcggccgg
      961 acctcgaagc cttatccgcc gccgcccaca gttccctttg aatggtttga atgtcggacg
     1021 aattgtcaag gaaaacgtcc cgttcgaggg tgggtattcc gagcgaccgc gccgttgccg
     1081 ccgcagtgct gcccggactc gtgtaggaat cgagaaagaa gaggttccga tccctgacct
     1141 cggcaagcac cgcacgcatc acccgttcgt ctctcgtcgc gcgagaaccc atgtggttgt
     1201 tcacccccgc cgcgccggga accgattcga ggtgacggcg cgttaattcc cgtatttcct
     1261 cgtcgcccat ggcggtcatg atggtgttcg gctccaacgg ctcgcttccg ttttcgggtt
     1321 ccatcggaag atgaagcaag atcgtctttc gagcggcgcg ggcgcgtttc gccagctcaa
     1381 gggtattctc cccggcggga agaaacgcga gattgacagg gaaagggaga tcagcgaatc
     1441 tgccagattc cgccatgctc cggtacccca tgtcatctat cactatcgcc agcaacccgc
     1501 gcgaggcaat tgaacggtcg gtggcaagaa gaatagaatc cgtcaccgca tgcgcggcac
     1561 cgaccgcgag cttcactttc aacggatcgg cgtactcatg ctcggcccgg tacacgtttc
     1621 cgccaaagga atggacaaca gaaatcactt cggcggttgc acgcagaact gagtaaccaa
     1681 caggcacgcg aacgtcccag accagtacgt caccggtagc gccacgattc cggggtttga
     1741 catcgccggc aaatcccgag ttttttagag atttcgcgaa ctctgttcgg aacctcacac
     1801 ggaagctgtc agaccgcgaa tcggactgca gaggcacccg gctcagccgc tgggcagata
     1861 ccgaattcgt ggcgtccggt cgagtgccct ccctcccgtt gtcggtcgcg cggatgccgg
     1921 ttgcgaggca cattgccact gcaagaaacc cgagaacgat agtccacggg aggacaattc
     1981 ccgaacgtaa cgtgcgccgc gaatccctca tcgggcatac cggaataaca ttgaccagac
     2041 gctccaggag tttcacgagg tgggcttggg ctttccgcac ccgcggttag aaagttacgc
     2101 ttcagtggcc ctcagatgcc acaccttgac cgagttcgcg gcacggcgta ccataaaata
     2161 cgaggggcgg ttagctcagt tggttagagc gcttgcttga catgcaagag gtcactggtt
     2221 caaatccagt atcgcccacc atttttttgc gccgtcccga ccgatcgggg gcggcgtttt
     2281 tttgtagacg tttttttcag aatagcgcgt ggaaccctcg gcggtgctcg ttgccgcaga
     2341 cgtatcaggt cacgacgccg cctgatcgct tcttgggttg ttcttgaggc ccgggcgatg
     2401 aacgttgacc ggtaaccgct atctttactt ttcggaaaaa ggatggtcgc ggcagacgtt
     2461 cgcgttcgga aactcccatg aaagaagagg cgcgagtctt caaggctctc gcggatccta
     2521 tccgccttcg cgtcgcgatc ctcctcgcgg tgcggggcga gacgtgtgtc ggcgatcttg
     2581 cggaagccct tggtgaggca gatttcaaga tatcccgtca tctttcggtg ttacgttcgg
     2641 cgcggctggt cgaagtgcgg agggacggga cgtggatgtt ctaccgtctg gcggagccga
     2701 agaacttgct ggagcgatcg ctgcaacagg ttttgcggac ggagatggcc gagcaccccg
     2761 ttatcgtcga ggatgtcgcg aaactgaccc acgcatccgg cgatgaacgg aaagcgcaga
     2821 agtcccctgc gcagattgaa cggcaggttc gggtcttgtt tctcggaaca ggaaacgcct
     2881 gccgcagtca gatggcggaa gcctgggcaa aaacactcaa gagccacgag atggaagtgt
     2941 tctccgctgg aatagaaaaa cagggactca gtcgtcgcgc cgtacgggtg atggctgagg
     3001 cgggggtgga tatcagcgca tacaggtcga aaacggtcgg cgaattaccc aatcgtgact
     3061 tcgacttcgt gatcacactg agcaacgcag cgcacgtcaa ctgcccgagg ttcccgggaa
     3121 acccgaaaat caactgcaat gtattcgacg atcctcccgc ccttgcggag ggatcacgcg
     3181 atgatgcgga agctctctcg cactaccggc gcgtccgcga cgagatacgg gcattcgttg
     3241 agacacttcc ggaagcgctg cagactttgt gaatcagcaa tcgggcgcgc gtcacgtttt
     3301 ggtgttccgc gtcgcggagc cgatcgcgaa gatgacagaa atcaaggacg gcgtgtcacg
     3361 gcgataaggc ccgcatttcc aagggtagac tacgcgactt tcgcggtgac cacgtgaatt
     3421 caggtatttc agttcccacg gaccggcgcc gagcgctctg aacggtctcg ttcacaacca
     3481 ggaaatctgc atggacgcat ttcgtattca aggcggcacg aggctggctg gctccgtgga
     3541 aatcgaaggc atgaaaaacg ccatactccc gatgatggca gcgtccatac tggcgaccga
     3601 aggcactctg gtacttgaaa acgtgcccca actgcgcgat gtcacggtga tgctgcgcat
     3661 tctcgaaaca ctcggcgtga agggagggta cgaccctcag aaaaggaccc tttcgctcga
     3721 cgccacagga gtcatcggcg acaccgcccc ctacgacctt gtcaggcaaa tgcgcgcgtc
     3781 gttcctcgtg tcgggggcct tggttgcgcg tctcgggcgg tgccgtgttt cgatgcccgg
     3841 cggctgcgcc atcggttccc gccccgtaag cgaccaactt cgcgcgttca aagcgctcgg
     3901 cggcgtggtc aacgaaatca aaggatacgt ggaggttgaa gcacgaaacc gttccggcag
     3961 gctcgttacg ctggattacc ctgcggccac cggaactgag aacatagtcc tgctggcgtg
     4021 cctgacagaa ggaacgacca ccatcgagaa cgcggcatgc gaccccgaag tggtggattt
     4081 tggagagtgc cttgtcaaaa tgggagctca gatcacgggc ctcgggacga acaccgtgcg
     4141 ggtgactggt gtcaaagctc tacgtgggac gcggcaccgg gttgttccgg atcgcattga
     4201 cgcagccacg ttcgccgtgg gggcggccat gacgcggggt gacgtgtttc tggccaatgc
     4261 aaggtcggat cacttcgaga tcgtgcttac aaagctggaa gaagctggcg ccatcgtcac
     4321 aagactggac accggtgtca gggttcaagg gcccgaccgc ctcaaatctg tcaacgtgcg
     4381 tacacttccc taccccgcgt tccccactga cgtgcaggca ccgatgatgg cggccatggc
     4441 aacggcggag ggggcaagcc ttatctggga acaggtgtac gataacaggt tcactcaggg
     4501 accggaacta cgccgcatgg gcgcaaacat caccatcgcg ggcgacaagg cggtggtggt
     4561 aggtgttccc gaactcaccg gtgctcccgt gatggcgagt gacattcggg cgggcggatc
     4621 gttggtgctc gcgggactgg ttgccaaagg cgaaaccacg gtatctcgcg tgtatcacat
     4681 cgaccgcggg tatgatcatc tcgaaacgcg gttgcagaaa ctgggggcgg tgattgagcg
     4741 gtacaacgag ccggcgtgat attcgcgcaa tcgattgtgg cgacgtccat gtgtgaggcc
     4801 tcgtttgtcg gcacagtgat tcggaagcac agaaacttct acgtcgtcga aagagacgcg
     4861 acggaagtcc tgtgcagcct gtcaagcaaa ctgcgtaaaa acctcgaata ccccgaagcc
     4921 gacatcagtt cccgccgcag gcgagtgcag gccgtgcaca aagtaggctt cgtggatcct
     4981 gtggttgtcg gcgacgatgt gcgcgtggag ccgggcgcgg gtatcggtct catagtcgag
     5041 cgactggaac ggcggtcagt cctttcgcgg gaagcccctt cgcgaacgca tctccagcag
     5101 gtaatcgcgg ccaacgtgga ccaagttctc gcagtggtga gtccgcaatc tccccctttc
     5161 cgtccggacc ttctggaccg tttgctggtc gggtgcgagc accacaacct gaagggcatc
     5221 gtgtgcctga cgaaaatcga tctcggtgtt cccggagaag tggaacgcgt gattgagaca
     5281 tatcctggaa tcggataccc ccttgttcga acgagcgcag tgacgggcga cggattgcag
     5341 gatctggagc aactgcttgc gggaagaaac acagttgcag taggcctctc gggaaccggg
     5401 aaatcgtcgc tcgtgaatgc gctggttccg ggagccaatc tcccggtagg acacgtgaac
     5461 aggaaaacgg gcctcggcac tcacagaacc accaccattt cattgcaccg attagcatcg
     5521 ggagggcatc tggtggatgc cccagggctt cgggaacttg gcctgtggga ttgttccgcg
     5581 gatcagcttc cagatctgtt tcctgaattc agaaatcttg tggatgcgtg ccgattccgt
     5641 ggatgcgcac acagggatga gcctggttgt gctgtaaaga ccgcggtgga agaaaatgcg
     5701 atcgcccacc accgttatcg gaattacctg tccatccgcg cggagctgga gcggcgcgaa
     5761 cagcagggat gatcaaccgg tcgacatgaa gtaatcccac acgagcttca caccggtaac
     5821 cagaacgaag aacagcacta tccatgcgaa gtgcttttgc ggaacctgcc ggttgaggcg
     5881 tacgccaagg agcgcgccca gcattacggc aggcgataac gttgccgctt tgacgatgat
     5941 cggccacgtt acaaggcccg caaacacgta cgcgggtagc ttcgtcagat ttgtcagggt
     6001 gaacacgatc ccgctggtgg ccacgaaggc ctggttggtc agtctttgcg gcaagaggta
     6061 cagcgttgtg acaagccctc cctggtgtgc aatcgcggag accgtcccgg tgacaaatcc
     6121 ggccagtgca ccgagaaaac ggtttcccgt gaacgcttcc gcatggggaa caagccattg
     6181 ccggagggcc tgaccgctgc cgaacaccat cgcgaggagg ccaatcacga cgcggaactc
     6241 attgtccggg atatgtccca gcaagatcat tcccagaccg atgccgacca caacgccggg
     6301 aaacagcgcc acgagattgc ttctgtccca tccacgccag taaaaggtga gcgtgagcat
     6361 gtcaccgacg atgaggagtg gcaacatcag gccgattccc tcgcgcggcg gcagggcaat
     6421 gacgaacatc ggtgacacga ttgctcccag accgccaccg aaaccggttt ttgagagtcc
     6481 gatggcaaac gccgccagga cggtgatgat gtcagaatgt gacagggcag gctccttggt
     6541 gcagggtgaa acgcggaagt tcaatattac gccgggtccg gcgacggaca caggcacgcc
     6601 gggtgcaacc gtggagaaca gggtgtgatt gagattaccc cgcgggatgc ggcacgtgcg
     6661 aggggggaca cgaagcggcg gtatgttacc gcgatgttcg acaggatagc gccacgttac
     6721 gacgcgatga acatcgtgat ttcgctcggg cagacgagca tctggcgcag acgtgctctc
     6781 aaaggaatct cgttgccaca ccgggctgtt attcttgatg tcggctgcgg caccgggtcg
     6841 gttattcgac tgctcaaacg tagatttcgc tctgcgcggt atctgggtat cgatctttcc
     6901 tcccggatgc tcgaaaaagc atcgcttcgg gaaccggaaa gcgatttctg tgtcggcgat
     6961 gcggggtcca ttccgtgcag gacggagacg tgcgatctca tcacgacatt cttcacgaca
     7021 aggaattttt ccgacctgcc cgccgccgtt cgtgaaatga tgcgagttct aaaaccggga
     7081 ggcaggttgg tcgttctcga cagttttcct gcgtctggca gccctctatg gcgcgttctg
     7141 aagaaaggct ggatgtatg
//
LOCUS       DAOTNG010000151         7158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9131_length_7158_cov_4.252006, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000151 DAOTNG010000000
VERSION     DAOTNG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_7158_cov_4.252006"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5179_08385"
     CDS             <1..677
                     /locus_tag="P5179_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95271.1"
                     /translation="LDAIAAELNACDAPVGICLFPWFFRLGFYALGMEGFLAAFYEQP
                     ALIHEMFGFWSDLVLHTIRPVLEKVKIDVLYLTEDLAYKNAPHVSPRMYEQFWLPYQN
                     PIIEECRRHGVELVCLWTAGNIVPLLPLVMRNGINCTWPLERVAGMDPAMLRKRFGRE
                     LLLAGGISKEALVAGPEAIDREIESLMPVIRDGGFIPALDDMPPAEVPLAHFAHMIDA
                     LRRIEA"
     gene            701..1609
                     /locus_tag="P5179_08390"
     CDS             701..1609
                     /locus_tag="P5179_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS95272.1"
                     /translation="MVTSQEGRDYYQKLCETPSFGANGFDFDGLRRGMATRREPTIPD
                     VQCIRMEINRIPCEWVMAKGADPAVRLLYLHGGGFISGSGAFYLPLAAHISAAAKCMV
                     LLPDYRLAPEHPFPAALEDCLSVFQALMEIEPEDEDSEPATFIAGDSAGGGLTLSTLL
                     ALRDRGMAMPNGGIALSPFADLTLSGDSLRSEAELDPIMHPNCLPDFVSRYIGGGDVR
                     DPRASPVFGDYTGIPPLLIQVGEHEIIRDDSVRTAEKARAAGVDVTLEVWPGMFHVFQ
                     SHDPLLPEGREAIEHIADFTRTRRGA"
     gene            1871..2716
                     /locus_tag="P5179_08395"
     CDS             1871..2716
                     /locus_tag="P5179_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95273.1"
                     /translation="MATMVIGYLVEWTKDDVVTAQFLTQMRRIHEDARAPCTLYCVAD
                     VIARHREQLRDIGADPLFDVQIATKRPLKTVCQHAEGKTIVWPGASLEEIETEVAEAV
                     GAFVECFGSRPTGISGPLGAYRGLQDRPDVLAVLDRHGIRFSRTYARDQNDWQPVEFS
                     VEPFWYTYQGFSHIAEFPSQGWQEPIIRKIYGWDDAHGYLDYVKADVDDAVRRENLVW
                     SYWACDWSAIRGATDMAHIEGLIRYAQENGLELSTQKAAYEKMVENETRAGNGDGSHE
                     ETPEP"
     gene            2770..4167
                     /locus_tag="P5179_08400"
     CDS             2770..4167
                     /locus_tag="P5179_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95274.1"
                     /translation="MGTQGLPRVTEIHHDPQLFVDDFLVDNRWADNHRAESVRRVFHQ
                     PAKHPANPVIAGDGGYVNVVRDDEAGLFRMWYQAYWDISYEPRKYTYAVAYAESPDGI
                     HWKLPRIRRHSFKNTLDNNIVFAGPFGDHASCQFIVDAPEEDRRGYRYVMLHGTRSAE
                     GHGMHLIGSHDGIEWDPASSSLIFPGYRPDTHSSILWDPRLRTYVCFTRATNLYDDTN
                     GLRRRVARLESPNLWDTWPIYPENVLIPDELDERSGHPLFYGMPTQYYAGIYWGFLWP
                     YAYGGDIHTELAFSRDGWNFQRLPERPRLVDRGPEGSWDAGMVFASTWLEVGDEWWLY
                     YSGTDGGHNSRENVPGIGLARVRKEGFVSLRSPAGGGTVVTKLMRWPGGRLLVNADAS
                     KSEFTVLATGYDRKPIDGFDPAPSLPVTGDRVRHEVVWAHGDIRALEGKPIRLEFLMR
                     GEVDLFGFQAVPEEA"
     gene            4170..5111
                     /locus_tag="P5179_08405"
     CDS             4170..5111
                     /locus_tag="P5179_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95275.1"
                     /translation="MGIAFSQVSDSAPWQPRYGLGVVVFAGRLWVLGGTASAANGTQF
                     NDVWSSEDGSEWRQELAAAPWSPRWNHATFAFGGKLWVIGGLASVDPLRNLNDIWSSP
                     DGRNWTLEVPDAPWAARHVWAWTIHRDRMFLIGGATDGSHTYRDVLSSEDGRRWRREA
                     VRDPWFCERKYHAAASCAGRLYVVAGVINDDSQPYGGRYLDDVWSSEDGAAWRCDASP
                     APWSGRAAPVLLAWRDRLWLSGGELQPRLYGNDLWWTEDGVHWQREADEPPWPGRTIC
                     GSAVFRDKVWIMGGHHRDWPERVGHSLNDVWTCEVVP"
     gene            5359..6459
                     /locus_tag="P5179_08410"
     CDS             5359..6459
                     /locus_tag="P5179_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95276.1"
                     /translation="MDLEQHLEEIRAGLKAKQFTNEASVSQGIILRLLMALGWPTWDT
                     RIVAPEYPVKGGRVDYALCDPPEKPIAFVEVKGVGQSEGAERQLFEYAFHAGVPVVIL
                     SDGQLWHFFLPAEQGNYDERRVYKLDLMERDVEESASRLMRYLQYESVKSHQALQAAQ
                     EDYRNMSRQRLMKETLPKAWAKLVEEEEGLLLELIADEVATLCGYKPDQATVADFVRG
                     NLCLPDTSGTKVQPRRTVATPNSAPVVLADDSRTLGGTGFVFRGNAYPARSAFHAMVQ
                     IFEEFRKIDPLYFERFAARKHGTKRRIIDREPARLFPGRPDLVRIHARKLSDGWWIGA
                     NVSRARIEQIIRMACEVAGVKFGSELIVNLGG"
     gene            complement(6607..>7158)
                     /locus_tag="P5179_08415"
     CDS             complement(6607..>7158)
                     /locus_tag="P5179_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95277.1"
                     /translation="RFVRDLLRAIADYNIAQVREALRYDIDAVYFGDDWGQQQGLQMG
                     YPLWREFIYPELKRMYEVVRSAGKFVVIHSCGDVDELFDDLVGIGLNCFNPFQPEVMD
                     VEDLMRRYRGRLAFFGGLSTQKTLPYGTVDEVKGESRRLLEMGREGGYIFAPAHAVEG
                     DVPLANMLAFIEAAQEQAGFAGA"
BASE COUNT         1357 a         2095 c         2382 g         1324 t
ORIGIN      
        1 acctcgacgc catcgcggcg gaactgaacg catgcgacgc gccggtggga atttgccttt
       61 ttccctggtt cttccgcctc ggattctacg cgctggggat ggaaggcttt ctggcggcgt
      121 tctacgagca accggcgctt attcacgaga tgttcgggtt ctggagcgat ctggtgctgc
      181 acaccatccg gccggtgctc gaaaaggtga aaatcgacgt cctgtacctc acggaagacc
      241 tcgcgtacaa gaacgcgcca cacgtgtcac cccggatgta tgaacagttc tggctgccgt
      301 accagaaccc gatcatcgag gaatgccggc ggcacggcgt ggaacttgtg tgcctctgga
      361 cggcggggaa cattgtcccc ctgctgccgc tggtgatgcg gaacggcatc aactgcacgt
      421 ggccgctgga acgggtggcc ggtatggacc cggcgatgct gaggaagcgg ttcgggcggg
      481 agcttctcct tgcgggcggc atctcgaagg aggcgctggt cgccgggccg gaggccattg
      541 atcgcgaaat cgagagcctg atgccggtga tacgcgacgg gggcttcatc ccggcgctgg
      601 acgacatgcc gcctgcggag gtcccgctgg ctcattttgc gcacatgata gatgcgctgc
      661 ggcggataga ggcttgaaag gtggggacga gggtgtgcga atggtgacgt cacaggaggg
      721 gcgcgactac taccagaagc tgtgcgagac gccatcgttc ggcgcgaacg gatttgattt
      781 cgacggactg cgcaggggaa tggcgacgcg gcgcgagccg acgattcccg atgtccagtg
      841 tatccgtatg gaaatcaacc gcataccgtg cgagtgggtc atggcgaagg gcgcggatcc
      901 cgcggtgcgg ctgctgtacc tccacggtgg cggattcatt tccgggtccg gggcgttcta
      961 tctcccgctg gcagcgcata tctctgcggc ggcgaagtgc atggtgctgc tgccggacta
     1021 ccgccttgcc cccgagcacc ccttccccgc ggcgctggag gattgcctca gcgtcttcca
     1081 ggcgttgatg gagatagaac ccgaggatga agattctgaa ccagcgacgt tcatcgcggg
     1141 agattccgcg ggcggcggcc tgacgttgtc cacgcttctg gcgctgcggg accggggcat
     1201 ggcgatgccc aacggcggaa ttgcgctgtc gccgtttgcc gacctgacgc tgagcggcga
     1261 ttcgcttcgt tcggaggccg aactggaccc catcatgcat ccgaactgcc tgccggattt
     1321 cgtgagccgg tacattggcg ggggagacgt gcgcgatccg cgcgcgtcgc ccgtgttcgg
     1381 cgactacacc ggaatcccgc cgctcctgat ccaggtcggg gagcacgaga tcatccggga
     1441 tgacagcgtg cggacggctg aaaaggcgcg cgctgccggc gtggatgtga cgcttgaagt
     1501 ctggcccggc atgttccacg tgtttcagtc gcacgatccg ttgctgccgg aaggccggga
     1561 ggcgatcgag catattgccg atttcacgag gacgcggagg ggtgcgtgac gacgggaaca
     1621 cgcccggggc gcatggtctt cgagtggcgc ggggaacgcc tcgcgttcca cgcgttgtat
     1681 cgaggagacc tgcaaggtgg gcctcgattc ccgccttcgc ttcgtgctcc atcgggcacg
     1741 cggaacgacg gcctcttttt gtctggaagg aaacgtcatt ctgagtgaag cgagcacgag
     1801 cgagcgaagt gaagaatctt ctgtttgtca caacctcgat ggcagaattc caggataagg
     1861 acacctatca atggcaacca tggtgatcgg gtatctcgtt gaatggacga aggatgacgt
     1921 cgtcaccgcg cagtttctca cgcaaatgcg ccgcatacac gaggacgcca gggcgccctg
     1981 cacgctctac tgcgtcgccg acgtcatcgc gcggcacagg gagcaactgc gggacatcgg
     2041 cgcagatccg ctgtttgacg tgcagatcgc cacgaaacgg ccgctgaaaa ccgtctgcca
     2101 gcacgcggag gggaaaacca tcgtctggcc gggggcgtcg ctggaggaga tcgagacgga
     2161 ggtcgcagaa gccgtcggtg cgttcgttga gtgcttcggc agccgtccca ccggcatcag
     2221 cggtccgctc ggcgcatatc gtggtctgca ggatcgcccg gatgtgctgg cggtgctcga
     2281 ccggcacggc atccggttca gccgaacgta tgcccgcgac cagaacgact ggcagccggt
     2341 ggagttctcc gtggaaccgt tctggtacac gtaccagggc ttttcccaca ttgccgagtt
     2401 cccgagccag ggctggcagg agccgatcat acggaagatc tacggctggg acgacgcaca
     2461 cggctacctc gactacgtga aggcggatgt ggacgacgct gtccgccgtg agaatctcgt
     2521 ctggtcgtac tgggcgtgcg actggtccgc gatacgcggg gcgaccgaca tggcgcacat
     2581 tgaggggctg atccgctacg cacaggagaa cgggctggag ttgagcacgc agaaggcagc
     2641 gtatgagaaa atggtggaaa acgagacacg ggcaggaaac ggcgacggtt cccacgagga
     2701 aacgccggag ccgtgagccg tgccgagacg ccctcaggcc cgatacggcc cacaggaaag
     2761 gcggcagata tgggaacgca gggacttccc cgtgtgacgg agatacacca cgatccccag
     2821 ctctttgtgg atgatttcct tgtggacaac cggtgggcgg ataaccaccg ggcggagtct
     2881 gtgcggcggg tgttccacca gcccgcgaag cacccggcga atccagtcat cgccggggac
     2941 ggcgggtatg tgaatgtcgt gcgcgatgac gaagccggcc tgttccgcat gtggtaccag
     3001 gcgtactggg atatcagcta cgaaccgcgg aagtacacgt atgccgtcgc ctacgccgaa
     3061 tcgccggacg gcatccactg gaagctcccg cgcatccggc ggcactcctt caagaatacc
     3121 ctggacaaca acatcgtgtt cgcgggtccc tttggcgacc acgcatcctg tcagttcatt
     3181 gttgacgcac cggaggagga ccgccggggg taccgttacg tgatgctcca cggcacgcgc
     3241 agcgcggaag gccacggcat gcacctcatc ggctcccacg acggcatcga gtgggacccg
     3301 gccagcagct cgctcatttt cccgggctac cggcccgata cgcactcgag catcctctgg
     3361 gacccccgcc tgcgcaccta cgtctgcttc acgcgcgcca cgaacctcta cgatgacacg
     3421 aacgggctgc ggaggagagt ggcgaggctg gaaagtccca acctgtggga tacgtggccg
     3481 atctatcctg aaaacgtcct gatccccgat gaactcgatg aacgctcagg gcaccctctg
     3541 ttctatggca tgccgacgca gtactacgcc ggcatctact ggggcttcct ctggccgtac
     3601 gcgtacggcg gggacatcca caccgaactc gcgttcagcc gcgacgggtg gaacttccag
     3661 cgactgcccg agcgccctcg tcttgtggat cgtggcccgg agggatcatg ggatgccggc
     3721 atggtgttcg cctccacgtg gctcgaggtg ggcgacgagt ggtggctgta ttactccggg
     3781 acagacggag ggcacaactc gcgggagaac gttcccggca tcggcctcgc ccgggttcgc
     3841 aaggagggat ttgtttctct gcgcagccca gccggcggcg gtacggtggt gacgaaactg
     3901 atgcgctggc cgggcggcag gcttcttgtg aatgccgatg cctcaaaaag cgagttcact
     3961 gtgctggcca ccgggtacga tcgaaaacca atcgacggat tcgacccggc gccgagcctt
     4021 ccggtcaccg gtgatcgtgt tcgccacgag gttgtgtggg ctcacggtga catccgtgct
     4081 ctcgagggga aaccgataag gcttgagttt ttgatgaggg gagaggtcga cctgttcggg
     4141 ttccaggcgg taccagagga ggcctgagcg tgggaattgc cttcagtcag gtgtcggact
     4201 cggccccgtg gcagcctcgg tacggcctcg gcgtggttgt gttcgccggc cggttgtggg
     4261 tgctcggcgg aaccgcttcg gctgccaacg ggacgcagtt caacgatgtc tggtcgtccg
     4321 aggacggttc cgaatggcgg caggagcttg cggctgcgcc gtggagcccc cgatggaacc
     4381 acgccacatt cgccttcggc gggaagctct gggtgatcgg cggcctggcg tcggtggatc
     4441 ccctgcggaa cctgaacgac atctggtcga gcccggacgg caggaactgg acgctcgaag
     4501 tgccggatgc cccgtgggcg gcccgtcatg tgtgggcatg gacgatccac cgggaccgga
     4561 tgttcctcat cggcggcgcg acggacgggt cgcacacgta ccgggatgtg ctgtcctcgg
     4621 aagacggcag gcgttggcgc cgggaagcgg tgcgggaccc atggttctgc gagcgaaagt
     4681 accacgccgc cgcatcgtgt gctggcaggc tgtacgtggt tgcgggtgtc ataaatgacg
     4741 actcgcagcc gtatggcggg cggtaccttg acgacgtctg gtcatcggag gacggcgccg
     4801 cgtggaggtg tgacgccagc cctgcgcctt ggtcgggccg cgccgcaccg gtgctgcttg
     4861 cctggcggga caggctgtgg ttatccgggg gcgagctgca accgaggttg tacgggaatg
     4921 atctctggtg gacggaggac ggcgttcact ggcagcggga ggcggacgaa ccgccgtggc
     4981 cggggcggac gatctgcggc agcgcggtgt tccgtgacaa agtgtggatc atgggcggcc
     5041 accaccgcga ttggccggaa cgggtgggcc acagcctgaa cgacgtgtgg acgtgtgagg
     5101 tggtcccgta gcggcgtgtt tggagtgcgc aggcaagccg gcgctttgaa agcggcggca
     5161 ggccgccgca ctccaaaaga acgcatcaga tcacccaagt gaaaccctgt tggcaacctc
     5221 ggacgtgttt gacgtacccg caccgagcct ttcctcgaag tagctttgga ccgacttgac
     5281 cgagaccggc attcctgtta tcaactagct caaatcacgg cacacaatca cacgcgattt
     5341 cgaatgaggg cgaagcgcat ggatctggaa caacatctcg aggaaatccg cgcaggcctg
     5401 aaagcgaagc agttcacgaa cgaagcgtct gtttcacaag gcatcatcct acgccttcta
     5461 atggcacttg ggtggcctac ttgggacacg agaatcgttg ctccggaata cccggtgaag
     5521 ggcggccggg tggactacgc gttgtgtgac ccgcctgaga agccgatcgc tttcgtagaa
     5581 gtgaagggtg ttggtcaaag tgaaggcgcc gaaaggcaac ttttcgagta tgcgttccac
     5641 gccggtgttc ctgttgtgat actctcagac gggcagttgt ggcacttttt cctgcccgcc
     5701 gagcagggca attacgacga acgacgtgtg tacaagcttg atctgatgga gcgcgatgtg
     5761 gaggaatcgg cctcacgtct catgcggtac ctgcaatacg agtctgtgaa atcgcaccaa
     5821 gcgctccaag ctgcgcaaga agattaccgc aacatgtcgc gtcaacgctt gatgaaggag
     5881 actctgccca aggcgtgggc caagttggtg gaggaggaag aaggcttgtt gttggaactg
     5941 atcgcggacg aagtcgccac cctgtgcgga tacaagccgg accaggcaac tgtggccgat
     6001 ttcgttcggg gcaacctatg cctccccgat acctctggca ccaaggttca gccaaggcgc
     6061 acagttgcta caccgaattc agcgcccgtg gtgcttgcgg atgattcccg gacattggga
     6121 ggcaccgggt tcgttttccg gggcaacgca tacccagcaa ggagtgcttt tcacgcgatg
     6181 gtccagattt tcgaagaatt ccgaaaaatc gaccccttgt acttcgagcg tttcgccgcg
     6241 cgcaaacacg gaacaaaacg gaggatcatt gacagggagc ctgcccgatt gtttccggga
     6301 aggcctgatc tggtccggat tcacgcgaga aagttgtcag acgggtggtg gattggcgca
     6361 aatgtcagtc gtgcgcgaat cgaacagatc attcggatgg catgcgaagt cgccggagtc
     6421 aaattcggga gtgagctgat cgtcaatctt ggcgggtagc ttcgctgctt aagtccggtg
     6481 tcaattgaaa gcgcaggcca gcctgcgcac tccaaagttt tgatcgttcg gtcgtctcga
     6541 tacagcggca tccgctgcgc gtcagccgcc actcgacgac cgttttgcgc acgacggagg
     6601 cgccccctac gcccccgcaa accccgcctg ctcctgcgcg gcctcgatga acgcaagcat
     6661 gttcgccagc ggcacatccc cttccaccgc gtgcgcaggg gcgaagatgt agccgccctc
     6721 acggcccatt tccagcaggc gtctgctttc ccctttcacc tcgtccacgg tgccgtaggg
     6781 cagtgttttc tgcgtggaaa gtcccccgaa gaaagccagc cggccccggt aacgccgcat
     6841 gaggtcttcc acatccatca cttccggctg gaacgggttg aagcagttga gcccgatgcc
     6901 gacgaggtcg tcgaagagtt catccacgtc gccgcaggag tgaatgacaa cgaacttgcc
     6961 cgccgaccgc accacctcgt acatgcgctt caactcggga tagatgaact cgcgccagag
     7021 cgggtagccc atctgcaggc cctgctgttg gccccagtcg tcgccgaaat agacggcgtc
     7081 aatgtcgtac cgcagtgctt cccgcacctg cgcgatgttg taatcggcga tggcacggag
     7141 gaggtcgcgg acaaagcg
//
LOCUS       DAOTNG010000152         7150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9138_length_7150_cov_5.561240, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000152 DAOTNG010000000
VERSION     DAOTNG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9138_length_7150_cov_5.561240"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1218)
                     /locus_tag="P5179_08420"
     CDS             complement(97..1218)
                     /locus_tag="P5179_08420"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRS95278.1"
                     /translation="MKKVTDIHQVDELFAEAREAGVPVLAPNFESFAELVAEFTAAQT
                     IAERFGIKRVPLMPSLTLSYPDRGNVAGRGGAYQDFAVPLNKRYSRDELNKFLRLGYH
                     VSNHDMDCFDGTSYFPNVYAIQTFDHGQPEGDKILLEDAKVLETVGVVMYDVTELLKR
                     RPELREDRKLALKTMAEATARYREAFGKYVRVESACDILPEAPAEGSLPNVEDTLTRP
                     DEMEEFLRISGADFFAPSIGTEHRAKNPEGTEKLYRESLARELHERVAKMPRYAPVDG
                     KIFSLHGSSCLDPSNKNNEGLYRKLHGMVIFRYYTGMANTMGSAVRQWVEDGERRVRT
                     NPKDGLHVNASSGRDWYVIQQLQPELVQVMINIGYAELA"
     gene            complement(1230..2021)
                     /locus_tag="P5179_08425"
     CDS             complement(1230..2021)
                     /locus_tag="P5179_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS95279.1"
                     /translation="MRGRLENRVALITGGARGIGKGIAARFLTEGARVAIADLNGEGA
                     TTTAAELGASSKGDCAIGVHVDVTAADSVRRAIEETRTRLGEVEILVNSAGFSKIARI
                     EDTTDEIWDRTLAINLKGVFLMCREVVPAMKQSGWGRIINLSSQSGKKGNSSYTAYCA
                     SKFGVIGLTQALAQELAEDGVTVNAICPGVVFTEMWGPEQVADYARKRNMAPEDVKEY
                     LTSKIPMKRAATVEDVAAVAAFLASDDAAYLTGQSYNVTGGTIMH"
     gene            complement(1994..3118)
                     /locus_tag="P5179_08430"
     CDS             complement(1994..3118)
                     /locus_tag="P5179_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRS95280.1"
                     /translation="MRAVVYGNDHKLSLQEMPVPSCPRNGLLAKVHACAICGSDVKMF
                     KAPDPRVKPPQIVGHEFVGEIVEVGSDAKGFAVGDRVVMATTLSCMACPACWAGKTNL
                     CPNVMPVSRAFPGAFAPYIAIPWEGIRAGNTVKVPSSLEDDGACLAEPLSCAVNAQQL
                     AGVRTGQTVVVVGGGPLGAINAELAKANGATRVIVSQRSATRQDLLRELDLDAVIDAA
                     SCDPVEEVKRLTNGRGADVVIVTAPDRTAQERALEMACKGGMVNFFAGMSKDDPMVTI
                     NSRLVHYNEISITGGSDSTARHVEIAVGLLAAGRVNWKKIVTHRFPLSRFFDGMQVML
                     DRAGLKVVIKPSGGRPVETSRGLFKPREANGLCEEGLKTV"
     gene            3437..4093
                     /locus_tag="P5179_08435"
     CDS             3437..4093
                     /locus_tag="P5179_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS95281.1"
                     /translation="MSELLFVEQFTRQSEMVTDEDLLAQSLDGSVNAFEVIVNRYKDR
                     LFNFVVRFVGDQETAEDIVQETFLRAYRNRDTFQAVAKFSTWIYTIAGNLAKSELRRR
                     KRWRFLSLGSKDEHGNTFELPDVASNPDRDAETLLAEAKIEDAIARLPDHYREAIILR
                     DIEGLDYDEISTIVGVPLGTVKSRINRGRLRLQEDLGDLACALWKQDEDETTAVRSNP
                     "
     gene            4231..4785
                     /locus_tag="P5179_08440"
     CDS             4231..4785
                     /locus_tag="P5179_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRS95282.1"
                     /translation="MTCAESQNLFSEYLEGQITGTVQQGLVEHLEACPACAQKLKLMR
                     IMRVRLSKLDRKHLPDTFSFEMRRALLAEMNRENIWLNRLRALLSPRPQTVWSAAVGT
                     AFAAVCFAVLWLTFPPGGQSGWRSDSVTEVDAAQHNQSVRYVLEHLPFDGEPIESTVK
                     DTARVMPVPPSQAREVVQPVSATF"
     gene            4795..5775
                     /locus_tag="P5179_08445"
     CDS             4795..5775
                     /locus_tag="P5179_08445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HRS95283.1"
                     /translation="MPNRVLKLVAALTVAVSADHAGSTVLQDMEREVSGIASRVASRV
                     VTVQASFRNPNDENAPGIVNVGTGLLLDSLGHVITASSVLSGMRVATPVMTVIDNGDR
                     THEALLYDVDSTLRIAVLYVPTMEAGPPIHAKRADWAPGYFALVVGNSFGVGPAVRMT
                     TVSGQRARDGFWQLSDPATPGFSGAPVFDSRGAFGGIIVGEVAGQAREPRPSPAVMVT
                     ARQLAPVMSRIAALSGNNNRSWLGLSVRPFVQTDGGTALVVSNVIGGSPAAQAGFLPG
                     DVVTRVDTVDVNFVSDLADWVRRSRPGYQATVRILRQGVEQDIRVTVGSR"
     gene            complement(5866..6351)
                     /locus_tag="P5179_08450"
     CDS             complement(5866..6351)
                     /locus_tag="P5179_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRS95284.1"
                     /translation="MQTDSANLALGLLETRGYTGLIAATDAATKTAGVEVRSIARATG
                     GLVLVALVGDVASVQVAVAAGLERAQAVGSPISAHVIARPDDQVWKSLGFPSPAEKGP
                     SPVAESAQPNTKEDDLDALPVRALRHMARSLPVGLTGRQISSASRDQILSAIRAARSH
                     K"
     gene            complement(6367..6639)
                     /locus_tag="P5179_08455"
     CDS             complement(6367..6639)
                     /locus_tag="P5179_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRS95285.1"
                     /translation="MLDALGMVETKGLVGAIEAADAMVKAANVTLIGKETIGGGYVTV
                     LVRGDVGAVKAATEAGSAAAQRVGELVSVHVIPRPHAEVEKILPKA"
     gene            complement(6798..>7150)
                     /locus_tag="P5179_08460"
     CDS             complement(6798..>7150)
                     /locus_tag="P5179_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95286.1"
                     /translation="VEWVNPYIRYGISGTDDIAKAAILESMYRAVVDGQPLRYGAENA
                     RRDQELVIATKESAWRGSEWVSLPLSGITRVEQRIHEVFREMYGCDPLGDVGTQLRAR
                     YSRLSAQWTVAGWL"
BASE COUNT         1392 a         2181 c         2043 g         1534 t
ORIGIN      
        1 cccggtgcgt cggggtcgaa tgtggaagga ggatgccaga caagcggagc tgtgcgtgta
       61 ttcattgttg ttatccctct cattcgataa tgcgtatcaa gcgagctccg cgtagccgat
      121 gttgatcatc acctggacca gttcaggttg cagttgctga atcacgtacc agtcccgccc
      181 gcttgatgca ttcacgtgaa gaccatcctt cgggttcgtc cttaccctgc gctcgccgtc
      241 ctcaacccat tgtctgaccg cgctacccat ggtgttggcc attccggtat agtaccggaa
      301 gatcaccata ccgtgaagtt tcctgtacag gccttcgttg ttcttgttgg acgggtcaag
      361 acaactggaa ccgtgcaggc tgaatatctt gccgtcaacc ggcgcgtacc ggggcatttt
      421 tgccacgcgc tcgtgtaact ccctggcaag ggattcccgg tacagcttct cggttccttc
      481 cgggtttttg gccctatgct ccgttccgat cgacggtgcg aaaaagtccg cgccggaaat
      541 cctcaggaac tcctccatct cgtcagggcg tgtaagggtg tcctcaacat tgggcaggga
      601 tccttctgcg ggcgcctcgg ggaggatatc gcaggccgat tcaactcgga cgtatttacc
      661 gaatgcctcc cggtaacgtg ccgtcgcctc tgccattgtt ttcaacgcga gctttcgatc
      721 ttctcgcagc tcaggccggc gcttcagtag ttcggtcacg tcgtacatga ctacgccgac
      781 cgtttcgagc accttcgcgt cttccagaag gatcttgtcc ccttccggtt gcccgtggtc
      841 gaaggtctga atcgcgtaca cgttagggaa atatgacgtg ccatcgaagc aatccatgtc
      901 atggttggaa acatgatacc ccaggcgaag gaacttattc agttcatcgc gggagtagcg
      961 cttgttcaac ggcaccgcaa agtcctggta cgctcccccc cttccggcca cgttcccccg
     1021 gtcgggatag gaaagagtca acgaaggcat caggggaaca cgtttgatcc cgaagcgctc
     1081 cgcgatagtc tgcgcagcag tgaactcggc caccagttcg gcgaagcttt cgaagttcgg
     1141 tgcgaggaca ggcacacccg cttcgcgggc ctccgcgaac aactcatcaa cctggtggat
     1201 gtccgtgact ttcttcatcg tgtgctccct cagtgcataa tggtgccgcc ggtgacgttg
     1261 tagctctgcc cggtgaggta ggcggcgtca tcggaggcca gaaacgcagc gacggccgcc
     1321 acgtcctcta cagtcgccgc acgcttcatg ggtattttgc tcgtgagata ctctttcacg
     1381 tcctcgggcg ccatgttccg tttcctggca taatcggcga cttgctcggg gccccacatc
     1441 tcggtaaaca ccactcccgg acagatcgca ttcaccgtaa ccccgtcttc cgccagctcc
     1501 tgcgccagcg cctgcgtcaa cccgatcact ccgaacttcg acgcgcagta tgccgtatat
     1561 gaactgttcc ccttcttacc cgattgggat gaaagattga tgatacggcc ccaaccgctt
     1621 tgtttcattg ccgggacgac ctcacggcac atgaggaaca cgcccttcag atttatggcg
     1681 agcgtgcgat cccagatctc gtccgtagta tcctcaatac gcgcgatctt ggaaaatccc
     1741 gcgctgttga caaggatttc cacctctccc agccgcgtgc gcgtctcttc gatcgcgcga
     1801 cgaacagaat cggcggcagt cacgtccaca tgcacaccaa ttgcgcaatc gcccttcgag
     1861 gatgcgccga gctccgcggc agtggtcgtt gcgccttccc cgttcaagtc ggcgatggca
     1921 acgcgggctc cttccgtaag gaatcgcgcc gcgatacctt tccctatccc tcgtgcacct
     1981 cccgtaatca gagctacacg gttttcaagc cttcctcgca taatccgttt gcctctctcg
     2041 gtttgaacag cccccgcgac gtttctactg ggcgtcctcc tgagggtttg atcaccactt
     2101 tcagcccggc gcgatccagc atcacctgca tgccgtcaaa gaaacggctc aacgggaacc
     2161 ggtgcgtgac gatctttttc cagttcacgc gtccggcagc aagcaggccc acggcaattt
     2221 ccacgtgtcg cgccgtggaa tcagagcctc cggtaatcga aatctcgttg taatgtacca
     2281 gccggctgtt gattgtcacc atcgggtcat ctttgctcat gccggcgaaa aagttgacca
     2341 tacccccttt gcacgccatc tccagagccc gttcctgtgc cgtccgatcg ggggccgtca
     2401 caataaccac gtctgctccc cgcccgtttg tcaggcgctt cacctcttca accgggtcgc
     2461 aactggcggc gtctattacc gcgtccaggt cgagttcccg caggagatcc tgccgcgtcg
     2521 ctgagcgttg gctcacgatc acccgggtgg cgccgtttgc cttcgcaagt tcggcgttaa
     2581 ttgcaccgag ggggccgcct cccacaacga ccacggtttg cccggtgcgt acgccggcaa
     2641 gttgttgggc gttgacggcg catgagaggg gctcggcaag gcatgcaccg tcatcctcga
     2701 gcgacgacgg taccttcacg gtgtttccgg cgcgaatgcc ttcccagggg attgcgatgt
     2761 agggcgcgaa cgcgcccggg aatgcacggc tgacaggcat cacgttcggg cagagattcg
     2821 tttttccggc ccagcatgcg gggcacgcca tacacgagag cgttgtcgcc atcaccaccc
     2881 ggtcgccgac cgcaaatcct ttcgcgtcgg agcccacttc cacgatctct cccacgaatt
     2941 cgtgccctac aatctgtggc ggtttcactc tggggtcagg cgctttgaac atctttacgt
     3001 cggaaccgca gattgcgcac gcatgaacct tcgcaagcaa accgttccgc gggcatgacg
     3061 ggaccggcat ctcctgaagg gagagtttgt gatcgtttcc gtacacaacg gcgcgcatgg
     3121 ggcactcctt acgtcttcag gttgttctgg caatgttctc cgaaccggcc attgggaaaa
     3181 atacacccgt ttgagaggcc gaccaacagc ggacggaccg tttttggatt ccctcggtgt
     3241 cagcgtccgc gagaaaaagg acgaagcgtt tcgcgtccgc tgaacacgtg ccttcgtcgt
     3301 atacttaatt ggggggcgac cccgagccga aggttgcttc tccaccaggt ccgcgagaac
     3361 tcgggaaccg aaatgccctt tgcacgtcct actattcgtt ggcagcgttc tccgaaccag
     3421 agcgaggggt ccgtgcatga gcgagttact tttcgtggag cagtttacaa ggcaaagcga
     3481 aatggtgact gacgaagacc tgctcgcaca atccctcgac ggcagcgtaa acgcctttga
     3541 agtaatcgtc aatcggtaca aagaccgcct tttcaacttc gtcgttcgtt tcgttggcga
     3601 ccaggagacg gcggaagaca tcgtccagga aaccttcctc cgcgcatatc gaaaccgcga
     3661 tacgttccag gcggtggcga agttctcgac gtggatctac accattgccg gcaacctggc
     3721 aaaaagcgag cttcggcgcc gcaaaaggtg gcgattcctg tcgctcggct caaaagacga
     3781 gcacggcaat acgtttgagc ttcctgacgt cgccagcaat cccgacaggg atgcggaaac
     3841 tctgctggcc gaagcgaaaa tcgaggatgc catcgctcgc ctgcctgatc actaccgcga
     3901 ggcgatcatc ctgcgcgata ttgaagggct ggattacgac gaaatctcca cgattgtggg
     3961 tgttccactg ggcactgtga aatcaaggat caaccggggg cgcttgcgtc tgcaggagga
     4021 cctcggcgat cttgcatgtg ctctctggaa acaggatgag gacgaaacaa cggcagtgcg
     4081 atctaatccg taaactgcga gtggctgcgt ttcgacctcg taccgcggtc aaaaccggtt
     4141 acggacggaa ccggcaatcc tgcggcgttg tctgaactac aaacgtgaac gggaggtctt
     4201 atctgattca atgcgattgg aggcaatccg atgacgtgtg ctgagtctca gaatctgttc
     4261 tccgagtacc tggaagggca gatcaccggc accgtgcagc aaggactcgt tgagcacctc
     4321 gaagcgtgcc ctgcgtgtgc tcaaaagctc aagctcatgc gcatcatgcg cgtgcggctg
     4381 tcgaagctgg accgaaaaca cctgccggat acgttcagtt tcgaaatgag acgggccctt
     4441 ctcgcggaga tgaatcgcga gaacatatgg ctgaaccgtc tccgtgcact gctctccccc
     4501 aggccccaga ccgtgtggtc tgcagcagta ggcaccgcat ttgcggcagt gtgctttgcc
     4561 gtactctggc tgacgttccc accgggcggg caatctggct ggcggtcgga ctctgtgacc
     4621 gaggtggatg cggctcaaca caaccagtct gttcgttacg tgctggaaca cctgcccttt
     4681 gacggtgagc ccatcgaatc cacggtgaaa gacactgcgc gcgtcatgcc ggttcccccc
     4741 tcgcaggccc gcgaggtggt ccagccggta agcgcaacgt tctgaattcc tgcaatgcca
     4801 aaccgggtat tgaaactggt ggccgcgttg acggtagctg tctctgccga ccacgccggt
     4861 tccactgtac ttcaggacat ggagcgcgag gtgtccggca tcgcctccag agttgcctcc
     4921 cgcgtggtga ctgttcaggc atccttccgg aatccgaacg acgagaatgc tcccggaatc
     4981 gtcaacgtgg gcactggcct ccttcttgat tccctggggc atgttatcac cgcctcaagt
     5041 gtgctttccg gaatgcgtgt cgcaaccccc gtcatgactg taatcgacaa tggcgaccga
     5101 acgcatgagg cgctgttgta tgatgtggac tcaacgctga gaatcgcggt gctctacgtg
     5161 ccaacgatgg aggcgggccc gccgatacac gccaaaaggg cggattgggc tccgggttat
     5221 tttgcgctgg tcgtgggtaa ctcgttcggg gtcggtcccg ccgttcggat gacgacagta
     5281 tccgggcaac gggcacggga tgggttctgg caactgagtg atccggcgac gccggggttc
     5341 agcggcgccc cggtgttcga cagccgcggt gcgttcgggg ggattatagt cggagaagtg
     5401 gccggtcagg cacgcgaacc gcggccgtcc ccggcagtca tggttaccgc gcgtcagctt
     5461 gcccccgtaa tgagcagaat agcggcgctt tccgggaaca ataaccgctc atggttgggc
     5521 ctgtctgtcc gtccattcgt gcagacggat ggaggcaccg cgttggtggt gagcaacgtg
     5581 attggcggga gccccgctgc acaggcgggc tttttgcccg gcgacgtggt tacccgggtt
     5641 gataccgtcg acgtgaattt cgtcagcgat ctcgcggact gggtgagacg gtcacgtccc
     5701 ggatatcagg caaccgttcg aatcctacgg cagggggtgg aacaggatat tcgggttacg
     5761 gttggttcta ggtgagtgtc ttacggcctg acagccggtg caacgaaaag ggcctcccga
     5821 cgaggaattc aatcgccggg gggccccttc aattcgtgat tctggttact tgtgagaacg
     5881 cgccgcacgg atcgcggaaa gaatctgatc gcgcgatgcg ctcgatatct ggcggccggt
     5941 cagcccgacc ggtaacgagc gagccatgtg ccgcaatgcg cgcacgggaa gcgcgtccaa
     6001 atcgtcttcc ttggtattcg gctgcgccga ttcagcaact ggagaaggcc ccttctctgc
     6061 tggcgacggg aagccgagtg acttccagac ctggtcgtcg ggccgcgcaa tcacatgggc
     6121 tgagataggg cttcccactg cttgagctct ctccagaccg gcggccacgg caacctgcac
     6181 agacgcgaca tcgccgacaa gcgcaacaag aacgagtcca cctgtcgcgc gggctatcga
     6241 acgaacttca acgcccgcgg tctttgtcgc ggcatccgta gctgcgatga ggccggtata
     6301 gccccgggtc tcgaggagcc cgagtgccag gttggctgag tcagtctgca tgggagcgaa
     6361 tcccgtctat gctttcggga gtatcttctc gacttccgca tggggacgcg gaattacgtg
     6421 cacgctcacc agctcgccta ctcgttgcgc tgcggcggaa ccagcttccg tcgcggcttt
     6481 cactgcaccg acgtcgccgc gaacgagtac cgtaacgtac cctccgccaa ttgtttcttt
     6541 tccgatgagc gtcacattgg ctgctttgac catcgcatcc gccgcctcga ttgcgcccac
     6601 caagccctta gtttccacca taccgagtgc atccaacatc ccgcatacct tcctttcgca
     6661 ggcaatctat tcaggacaga acgtgttcaa atgctcaagt tcctgtgcta atggtagcgg
     6721 cactgaacaa ataggtcaag ggagcacacg tgcgcgcacg aaatttctcc gcgcatgccc
     6781 ctgtcacatt cagctgctta caaccatcca gcgacggtcc actgggcgct caggcgcgag
     6841 tatcgcgcac gcaactgggt gcctacatcg cccaggggat cacaaccgta catctcgcgg
     6901 aaaacctcat ggatccgctg ctccactcgt gtgattccgg agagcgggag ggaaacccat
     6961 tcactgccac gccacgcact ttccttcgtc gcgataacga gttcctgatc gcggcgggcg
     7021 ttctccgcgc cgtaccgaag aggctgcccg tcgacaactg cgcggtacat gctctcgagg
     7081 atcgccgctt tcgcaatatc gtccgttccg gagatgccgt agcgaatgta gggattgacc
     7141 cattcaacga
//
LOCUS       DAOTNG010000153         7144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9152_length_7144_cov_5.722528, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000153 DAOTNG010000000
VERSION     DAOTNG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9152_length_7144_cov_5.722528"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="P5179_08465"
     CDS             complement(<1..786)
                     /locus_tag="P5179_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95287.1"
                     /translation="MKYAPFFLVAGAAAFLYAAHVPAQEIDNTGTDVPAWVDADGDGI
                     NDVAAMRRERRGGNENAYAESRRQLMAELTANLTDEQKAELTALRNQLREDHATPEAV
                     QAALGEKLQSFGITLPDAWYQTRLEFEGGFTLTAEQRAQVQALMESLRTDGKTPEERR
                     AAVVELLTSYGITVPDNFMAPPERRNGPRGDRGRGPILTEEQRTAVDALVASMKEEGK
                     TRAEIQAAVGELMASYGITAPVRRGPPDGTQAARSGLTNQQKAE"
     gene            complement(1208..1963)
                     /locus_tag="P5179_08470"
     CDS             complement(1208..1963)
                     /locus_tag="P5179_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95288.1"
                     /translation="MHKSAFFLLSGAAILIMAGASHAAGNSPQRPASSMVAAELPAVD
                     MDTTVFVDADGDGINDRMPRMRRDDLRALDQEREQSRGQMLNQAMAGLTKEQKDEIKQ
                     LRTAMRRQGASPDQIHAAVGEKIRSYGVAVPADWDLTANEYANARRMTAQRRAQVQAL
                     ADSLRAEGKSPAEIRAAIAAEYSPGYAGPRGSKAMGRDTGRARQDSLRAAGRPQMPAG
                     RDSVRQPMRQAGRDTTEARGRAPRPERGGGRRR"
     gene            2208..3233
                     /locus_tag="P5179_08475"
     CDS             2208..3233
                     /locus_tag="P5179_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95289.1"
                     /translation="MRILILLLVVLGLCAGNATCDTLVERSGRELSGSLVGVQDSILV
                     FRTDAGDTTRIARSAVERVTFFAPVHGHLGTVQLDRSGRVELVVFGTGYGILNGALLA
                     SIADFGVDGFTLSMLAGGGAGLYVPWQWSKTRPVSDARASLISFAGSWGMWQGIGWTN
                     ALVDEPSADAAITAGLLTGGAGILATAALTGQHRISSGDATFITTAPAWSSAYWLWVM
                     VLTGTENGRFVLGTTLAAGDIGVATGVLLADRIEASQGRVRLANLGGIVGGLVGGAAV
                     ALTNVDSPKLGVSVVMTAATLGLGAGWHLTRNYDRIDAAVSRLEVAPLLVAGNRPVRG
                     LQAAYSF"
     gene            complement(3239..4198)
                     /locus_tag="P5179_08480"
     CDS             complement(3239..4198)
                     /locus_tag="P5179_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRS95290.1"
                     /translation="MKLRDEVAVEHTIAGWALIPTRQETFDPAWREVRPAGAVWESQR
                     VPVPHGNEAILSWNVPPEVQPGLAFEMSGQVRGRWSGWTRVGEWGEAPAPGVDDPLLR
                     REVDTVVWDAHVDELRVRVRTATGEHLKLDRLVLAFPAAAPKESIALPTTFNQELPFR
                     SQRTEDESIGDRICGATCVAMALAPRVPNVTAEAIAKAAYDPVHDIYGNWARLAAAAS
                     QHGAVAWVQRLASLDDVFARLVAGYHIICSVAFENGKLTGAPVQNTSGHLVLIRGWNK
                     KGDVVCNDPAFTDSRGDGVVYRRSEFERLWLTHGGAAIILRWE"
     gene            complement(4182..6365)
                     /locus_tag="P5179_08485"
     CDS             complement(4182..6365)
                     /locus_tag="P5179_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS95291.1"
                     /translation="MTRSLSVFLSRAGDRVSLCLTAIALAGMFLLAGCRITDRPAVTV
                     TTVDAQNGVRIASMPAVMVGGFGVGAGQVQSVGPVAVTEDGQLYIADNTQRRITVISA
                     ETGPLPTRSYLPGLANPLVPGDTVHVVRAMEWHGPTLYLVDRARIWAIPRSAGPGAQA
                     SAIEPEGGVADVRDIEVAADGSLYLLSERGIRILSPSGGLRDSILVRGETPPRCVAFS
                     FGPDSNLYISTRSLNHVVVYNRSGQLVKRIGAGTFAGEARGIAVDQFGNVFVRDEKEA
                     AIRAFSADGTYRGKIGARGSGPGQMLSAEQLVIDRGRRVLVVPDPTNYRVQVFDLTGR
                     RAEARDLVTHTPAFERTHRPYHITLGMGVDASTQLQIAWKTAPASTGSMVVYVPVDGG
                     DISAVDWSEPVVKTARGSSTAFVSNIGPYRAHSVALTALEPGTRYAYRVGDGSSEGWS
                     ETNTFLTPPASPESLVVAILGDSRNRMDVWGNIVNRAAAFDPAFIVTTGDLVSNGEDF
                     THWDPWFHEADAVFKKTPFMTTPGNHERESPNYYLSFEMPRNAPAHLQEKCFSFDVGQ
                     THWISLNTQVDLAPQTAWLEEDLRKNTKPWVFVFLHRPAYAAHASRGDGNRDVREAWG
                     GLFDQYGVDVVWQGHDHYYHRSKPIRAARVVPEGRGPIYITTGGAGAPLYQMKPNQYT
                     EVGEITDHFCMMTLRGNHCEVVVYRADGSVADRFSVRDCPRETAR"
     gene            6598..6948
                     /gene="ytxJ"
                     /locus_tag="P5179_08490"
     CDS             6598..6948
                     /gene="ytxJ"
                     /locus_tag="P5179_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRS95292.1"
                     /translation="MVDITSVEQAEALISGAKERPLIVFKHSTRCPVSGRAAGEVEAF
                     SRTPEAAGVTLARVLVVEDRPVSLWLAERLQTPHASPQALLVADGKVVWTASHFGVTA
                     DAMASALRGLAGKG"
     gene            6951..>7144
                     /locus_tag="P5179_08495"
     CDS             6951..>7144
                     /locus_tag="P5179_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95293.1"
                     /translation="MEDYPPEVARRLSRLRWTAALLWIGWTIVAVPIYLAYRYYPMLF
                     GVPHLKIGAITLLSLGAVWF"
BASE COUNT         1247 a         2263 c         2236 g         1398 t
ORIGIN      
        1 ctctgctttc tgctggttcg tcagtcccga acgggctgcc tgtgtgccat ccggcggacc
       61 ccgccgaact ggtgcggtga tgccatatga cgccataagt tcgcccaccg cggcctggat
      121 ttccgccctg gttttgcctt cttccttcat gctggcaacc agcgcgtcta cggccgtgcg
      181 ctgctcttcc gtcagaatgg ggccgcggcc acggtcgccg cgcggaccat ttctccgttc
      241 cgggggtgcc ataaagttat ctggtacggt aatgccgtac gatgtaagca gctcaaccac
      301 tgcggcgcgg cgttcttccg gcgtcttgcc atccgtcctc agcgactcca tcaacgcctg
      361 aacctgtgcg cgctgttcgg cagtcaatgt gaacccgcct tcgaactcaa gccgtgtctg
      421 gtaccacgcg tccgggagag tgatgccgaa tgactggagt ttttcgccca gtgcggcctg
      481 aacagcttct ggggtggcgt gatcttcgcg gagttgattc ctgagcgcgg tcagttccgc
      541 cttctgctca tcggtcagat tggcggtgag ttccgccatc aactgccggc ggctctcggc
      601 gtacgcgttt tcattcccgc cacggcgttc gcgccgcatt gcggcaacgt cgttgatccc
      661 gtcgccatcc gcgtcaaccc atgcgggcac gtcggtgccg gtgttgtcga tttcctgcgc
      721 aggcacgtga gcggcataca gaaacgccgc cgctccagcg acgaggaaaa acggtgcgta
      781 tttcatggtt cgtgctcctt accctgtgtg tgatggtgcg aaaacccatt gctctgtgtg
      841 cgcaatctgg tggccaacca gtttcggtga aacagcgctc gtgatgagcg cctcgcttcc
      901 cggctcccag ttcttgcaac gggtgtgcca acagggagtc tcacgcggag cgaaagcaac
      961 taagatattg ttcataatta ttttgcgcgg cgacatcgcg tccgtccgga atactgtctg
     1021 gggccgcgcg ctcccggagc atgccggagg cagcgccggt gcgatgagtg tggcggcaaa
     1081 cgggatgccg ttgccctgcc ggaagcgtac cgatgcgtac gcaccgttcg gaacaggtag
     1141 gcaggaaaaa cgaaaaaccg gggtgacagt tcaccccggc ttcgtcgaga acgctctcca
     1201 atcgatacta acgcctgcgt ccgccgccgc gttccggacg aggtgccctg ccgcgggctt
     1261 cggtggtatc acgaccagcc tgccgcatgg gctggcgcac gctgtcgcgc ccggctggca
     1321 tctggggacg ccccgcggcg cgcaggctgt cctgcctggc tctacccgta tcgcggccca
     1381 tggctttgga tccgcgcggg ccggcgtacc cgggggagta ttccgcggca atagcggcgc
     1441 gaatttccgc gggagacttg ccctccgccc gcagcgaatc ggcaagtgcc tgcacctgcg
     1501 cgcgccgttg cgccgtcatg cgccgtgcgt ttgcgtattc gttggccgtc agatcccagt
     1561 cggccggaac ggccactccg tacgaacgaa tcttctcccc gaccgccgcg tggatctgat
     1621 ccggcgacgc gccctggcgc ctcatcgccg ttctcagctg cttgatctcg tccttttgct
     1681 cttttgtcag gcccgccatg gcctggttga gcatctgccc gcggctctgt tcgcgctcct
     1741 gatccagcgc gcggaggtcg tcgcgtctca tcctcggcat tctgtcgttg atgccgtccc
     1801 catcggcgtc aacaaagacg gtcgtgtcca tatctaccgc cggcaattcg gcggcaacca
     1861 tggaactcgc gggcctctgg gggctgttgc ccgccgcgtg ggacgcgccg gccatgatga
     1921 ggatggcggc gcctgaaaga aggaaaaacg ccgatttgtg catcgcgatg ctccttcatg
     1981 taatgctggt ggcagcgcag ccggaatggc tgcgccagaa aaatgaacgt accgtacaat
     2041 ataacggcct gaacagaaga tagcggtgat ttccgtccgg cagcacaaaa atgccgacgc
     2101 ccgcgaaggc gtttggagaa ggttgcatcc gtgcgtaccc tcactcccac cgggcgcatg
     2161 ctggtttcag ttgtccaacg ggtctctaca gatccgggga gtttcgaatg agaatactga
     2221 ttcttctgct ggtggtcctc gggctctgcg ccggcaatgc gacgtgcgac acgctcgtcg
     2281 aacgctccgg acgggagctt tccggatccc ttgtgggcgt tcaggactcg atccttgtgt
     2341 ttcgcacgga cgcgggcgat acgacacgca tcgcacgctc cgccgtggag cgggtgactt
     2401 ttttcgcgcc ggtgcacggg cacctcggca ctgtgcagct tgaccgctcc ggccgggtgg
     2461 agctcgtggt gttcggcacg gggtacggca ttctgaatgg tgctcttctg gcctccattg
     2521 ccgatttcgg cgtggacggg ttcacattgt ccatgctggc cggcgggggc gcggggttgt
     2581 acgttccgtg gcagtggagc aaaacgcgcc cggtcagcga cgcccgcgcc tcactcatat
     2641 cgtttgcggg cagctgggga atgtggcagg gaattggctg gacgaacgcc cttgtggatg
     2701 aaccctcggc agatgccgcc atcaccgcag ggctccttac aggtggcgcg ggaatactgg
     2761 caaccgccgc actcaccggt cagcacagaa tatcctcggg ggatgcgacc ttcatcacca
     2821 ctgcgcctgc gtggtcaagc gcgtactggc tctgggtgat ggtcctcacg ggtacggaaa
     2881 atggccgctt cgtgctcgga accacgcttg cggccggcga tattggtgtc gcgacgggag
     2941 tgcttctggc cgacaggatc gaggcgtcgc aggggagagt tcggctggcc aaccttggcg
     3001 gcatcgtggg aggattggta gggggggccg ccgttgcatt aacaaacgta gacagcccga
     3061 aactgggagt aagcgtcgtg atgacggcgg cgacgctggg cctgggcgcc gggtggcacc
     3121 ttacccgcaa ctacgaccgc atcgacgctg cggtttcgcg ccttgaggta gcacctctgc
     3181 tggttgcggg aaatcgcccc gtccgcgggc ttcaggcggc gtactcgttc tgaaccgcct
     3241 attcccagcg cagaatgatc gcggccccgc cgtgcgtaag ccagaggcgc tcgaactcac
     3301 tgcggcggta caccacacca tcgccgcgcg agtcagtgaa cgccgggtcg ttgcacacca
     3361 cgtcgccctt tttgttccaa ccccggatca ataccaggtg cccggaagtg ttctgcaccg
     3421 gcgcacccgt gagtttcccg ttttcaaacg ccaccgagca gatgatgtgg tatcccgcca
     3481 cgagtcgtgc gaacacgtca tcgagcgatg ccagacgttg cacccatgcg accgcgccgt
     3541 gctggctggc ggctgccgcc agacgcgccc agttgccata gatatcgtgc accgggtcgt
     3601 atgcagcctt cgcgattgct tccgccgtga cattgggaac ccggggcgca agcgccattg
     3661 caacgcacgt tgcaccgcaa atgcgatcac cgatgctctc gtcctcggtt ctctgggagc
     3721 ggaacggaag ttcctgattg aacgtggtgg gaagagctat gctctctttc ggggccgccg
     3781 ccggaaacgc aagaacgagc cgatcaagtt tcaggtgttc gcccgtcgcg gttcgaaccc
     3841 gcacgcgaag ctcgtccacg tgggcgtccc acactacggt gtccacctcg cgccggagga
     3901 gaggatcgtc gacgccgggc gccggggctt cgccccactc ccccacgcgc gtccaacctg
     3961 accacctgcc gcgcacctgc cccgacattt cgaacgcgag cccgggctgc acctccggtg
     4021 gcacattcca cgagagaatg gcttcgtttc catgaggaac cggcacgcgc tggctttccc
     4081 acacggctcc tgcggggcgc acttcgcgcc atgccggatc aaacgtttcc tggcgcgtcg
     4141 gaatgagcgc ccagcctgcg atggtgtgtt ccacggctac ctcatcgcgc agtttcacgc
     4201 ggacagtctc tgacggagaa gcgatctgcc acggatccat ccgctctata cacaacgact
     4261 tcacagtgat tcccgcgcag cgtcatcatg cagaaatggt ccgtgatttc gcccacttcc
     4321 gtatactgat ttggcttcat ctgatacaac ggagcgccgg cgccgccggt ggtgatgtag
     4381 atcggcccac ggccttccgg aacgacgcgg gcggcgcgaa tgggctttga ccggtggtag
     4441 tagtggtcgt gcccctgcca gaccacatca acgccgtatt ggtcaaacag gccgccccac
     4501 gcctcgcgta cgtctcgatt tccgtctccc cgtgaggcgt gcgcggcgta cgccgggcga
     4561 tggagaaaca caaacaccca cggtttcgtg tttttcctca ggtcctcttc cagccacgcg
     4621 gtctgaggcg caaggtccac ctgtgtgttg agagaaatcc agtgcgtctg gcctacgtca
     4681 aaactgaagc atttctcctg gaggtgggca ggcgcatttc tcggcatttc gaacgaaagg
     4741 tagtagttgg gggattcccg ttcgtggttg ccgggggtcg tcatgaacgg agtcttcttg
     4801 aacacagcgt ccgcttcatg aaaccaggga tcccagtgcg tgaaatcctc gccgttgctg
     4861 accaggtcgc ctgttgtgac gatgaaggcc ggatcaaacg ctgcggctcg gttcacgatg
     4921 ttgccccaca catccatgcg gttgcgcgaa tccccgagta tggcaaccac aaggctttcc
     4981 ggagacgcgg gcggggtgag gaacgtattc gtttcgctcc atccctcgct cgatccgtca
     5041 ccaacacgat aggcgtagcg agttcccggt tcaagcgcgg tgagggcgac ggagtgcgct
     5101 ctgtacgggc cgatattcga gacgaacgcg gtactgctgc ctcgcgcggt ctttaccacc
     5161 ggttcgctcc agtcaacggc agagatgtct ccaccgtcca cgggaacgta gacgaccata
     5221 ctgccggtgg aagcgggggc agttttccag gcgatctgaa gctgcgttga cgcgtccacg
     5281 cccatcccga gagtgatgtg gtacgggcgg tgcgtgcgtt cgaacgcggg ggtatgggta
     5341 acaagatcgc gagcctcggc tctgcggcct gtcagatcaa aaacctgcac gcggtaattg
     5401 gtcgggtcgg gaacaaccag cacgcgccgc ccacggtcaa tgacaagctg ctctgcagag
     5461 agcatctggc cggggccgga cccccgcgcg cctatcttcc cccgatacgt gccatccgcg
     5521 ctgaaagccc ggatcgccgc ttccttctcg tcgcggacaa acacattccc gaactggtca
     5581 accgcgatcc cgcgcgcttc gcccgcaaac gtgccggcgc cgatgcgctt caccagttgg
     5641 ccactgcgat tgtagacgac cacgtggttc agcgatcgcg tgctgatata aagattgctg
     5701 tcaggcccga aggaaaacgc gacgcaacgg ggcggcgttt caccacgaac gaggatgctg
     5761 tcgcgaaggc cgcccgacgg ggagaggata cggatgcccc tctccgaaag gaggtacagg
     5821 gacccgtccg cggccacttc aatatctctc acatcggcca ctccgccttc cggttctatc
     5881 gcgctggcct gtgcgccggg ccctgcggaa cggggaatgg cccagatgcg tgcgcgatcg
     5941 acgaggtaga gtgtgggccc gtgccattcc atggcgcgga cgacatgcac cgtgtcgccg
     6001 gggacgagcg gatttgcgag ccccggaaga taggagcgtg tcggaagagg gcctgtttcg
     6061 gcagaaatga cggtaatacg ccgttgcgtg ttatccgcaa tgtaaagctg tccgtcctcg
     6121 gtcaccgcca ccgggccgac gctctgcacc tgccccgcgc ccacgccgaa cccgcccacc
     6181 atcaccgcgg gcatgctggc gatgcgcacg ccgttctgcg cgtccaccgt cgtcacggtc
     6241 actgccgggc ggtcggttat ccgacaaccg gcgagcagga acatccccgc aagcgcaatg
     6301 gcggtaagac acagcgaaac gcggtcgccg gcgcgggaaa ggaaaacgga aagggaacgc
     6361 gtcatgtgcc gctcctgata gacctgaaaa caggtaatac gggaagttct gtgggaaatc
     6421 ctctgtaaca taaagttaag caaaacgggc ggatacggtc aaggaatacc gtccgcccgc
     6481 agggcggacg cggcgtctgt gcgccatccc gtcgcgagcg acctgaccac gtctcctcgg
     6541 cgttcctttt ttgcgacatt taggtactac cccgttggca cacaaggaga accacagatg
     6601 gtcgacatca cctcggtcga gcaggcagaa gcgttgatat cgggggcaaa ggagcgcccg
     6661 ttgatagtgt tcaagcacag cacgcggtgc ccggtaagcg ggcgggcggc aggcgaggtg
     6721 gaggcgtttt cacgcacgcc tgaagccgcc ggtgtcaccc tcgcgcgcgt gcttgtcgtg
     6781 gaagaccggc cggtttctct ctggcttgcg gaacggttac agaccccgca cgcctctccg
     6841 caggcgcttc tggtggcgga cggcaaggtc gtgtggacgg catcccattt cggtgtaact
     6901 gccgacgcta tggcatccgc cctgcgcggg ctggccggaa aggggtgatc atggaggact
     6961 acccgcccga agttgcccgc cgccttagcc gtctgcggtg gacggcagcc ttgctctgga
     7021 ttggctggac cattgtcgcg gtacccatct accttgccta ccgctactac ccgatgctat
     7081 tcggggtccc ccacctgaaa atcggcgcca tcaccctgct ctcgctcggc gcggtctggt
     7141 tcat
//
LOCUS       DAOTNG010000154         7078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9249_length_7078_cov_5.678200, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000154 DAOTNG010000000
VERSION     DAOTNG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_7078_cov_5.678200"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="P5179_08500"
     CDS             <1..315
                     /locus_tag="P5179_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95294.1"
                     /translation="TWDTATRREDVQSMAVRFGAAYEQPLPRINSAVTVAAAKIGDGG
                     AWGVEYSFARQIMLRVGDSGIGEGSFAGGAGFRWRNVDLDYALQRHPLGTSHRVSVQY
                     HH"
     gene            290..1174
                     /locus_tag="P5179_08505"
     CDS             290..1174
                     /locus_tag="P5179_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95295.1"
                     /translation="MSVFNTITRVGLVGLLVPVVALLVNWSCSPQKSNSRTLARPVWE
                     CPVPPSSEVEAGPSASPLDDFVHLRWIVGSDDDLDGYFIHRRKSTDLIDTLFATVYLT
                     RDQLANRGSTIEWVDRNSRVGSVYYYVLFAFDKKGNRSARSDTVGFGTLEKPMIYGPS
                     QNQVVVDSRPVFQFGPRTTALYVQSYVVRVVSDSANSQVLWVSPRRPLLGYTSGERTT
                     IQYGNGGFTRRPFLNPGKYRWRVDFQGSYPFVDIDAPCGCVYDTVTCPGASPTLPRPE
                     DLSFISSQSAWVPFTVAP"
     gene            1187..2068
                     /gene="ispE"
                     /locus_tag="P5179_08510"
     CDS             1187..2068
                     /gene="ispE"
                     /locus_tag="P5179_08510"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRS95296.1"
                     /translation="MVPSITARAPAKLNLGLSIVGRRADGYHDLISVFHAVDWQDTLE
                     IVEIPSGIEFACSSCDGVPGAPQNLVFRALELVFQRFRITRGVAARLQKEIPAGAGLG
                     GGSSDAAATLRAVRRLFNLQGDEDSWLLLCQQLGSDVPFFWRGGTALVEGRGERLTPL
                     SIDERLWFVVVVPPIHISTAWAYGQLSKPSGGNQDYRDRIQRLRAGELSLSDFCREIP
                     NDFERVVEAAFPRIGEIRRALEASGAISARMSGSGSSVFGVYSKQEEAQRAAALFPSD
                     CLVRCVQGMPSSRDQML"
     gene            2131..3915
                     /locus_tag="P5179_08515"
     CDS             2131..3915
                     /locus_tag="P5179_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95297.1"
                     /translation="MIQYDNPKPLLQRFKVIVPYVRPYTPYILGALGLTVVLTVLALL
                     PPLIMRALIDRVLTAGNWRILLPIIVAHALVPIMSGLISFFNTLVIAYAGRRLVFDVR
                     TQMYRHLLRLSMRFHGEMSSGAVMSRLMSDVNQVQNLIRENSITLVTDIVSFMFAGFV
                     TFSLNWKMAILLWIMLPLYVFNSRFFVKRIRRSNQRMRRTMDKIAGSLQERLSGTRRV
                     RAFGREEDETEQFLEQTEENLAHSMSGTIHSVSFSTASRLVWGFGTTILFVLGVYFAL
                     HGQMTYGSVTAFMAYVSQMFGPALRFTELANQLEQVMVSVDRIFEVLNTEPDVVEKPD
                     AVVLTSVVGHIQFDHVTFGYKPDEPVIKDVCLEIPPGKMVALVGHTGCGKTTITTLLM
                     RLWDVQEGSIRVDGHDIRDVTFASLRRSIGVVLQDPVLFNATIADNLRYGCPEATMDQ
                     VIEAAKAAEIHEMIMNQPEGYNTELGGESGIKPSVGEKQRMAIARAIITNPAVLILDE
                     ATSALDSQSEMLIQKALSRVMKNRTSLVIAHRLSTIVEADMIVVMDQGRVIETGSHAE
                     LLKAGGHYAGLFNQQRGTGKPAGVTAGS"
     gene            4059..4136
                     /locus_tag="P5179_08520"
     tRNA            4059..4136
                     /locus_tag="P5179_08520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4094..4096,aa:Pro,seq:tgg)
     gene            complement(4197..5876)
                     /locus_tag="P5179_08525"
     CDS             complement(4197..5876)
                     /locus_tag="P5179_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRS95298.1"
                     /translation="MQSNAMHARKLALVGLFLAYFASGCSLILRLPPSTTHMVRMRDG
                     TELATDVYFPKGRLHRMPTILIRNTYNKGAVVNGIKTSSVIAKTFTDKGYAVVVQDTR
                     GRFASAGVDSIFRTDGAGPNQDGYDTIEWITRQNWSNGAVGMWGMSALGITSYMAVAS
                     GHPALKAAYVVMCGSNLYDDVFFPGGVFRKDLVERWVTGQGRADYLPVLREHASYDDY
                     WEAMNLSVHAPRAHTAVYHCGGWFDVMSAGQTNGFQALHERGGEGARGRQLMVMGPWS
                     HHGMGRKQGDIEFPGNNKDVNPMGDAVAWFDSRLKGVPTRVDSVAAVRYYLMGASGDS
                     SGAGNRWLNAPTWPPPESQDVAFYLAEDRALTRDAPTSEAGALSYEFDPRNPVPTLGG
                     LNLYPPIGPRDVRSVESRPDVLSFTTPPLEAPVAIAGNVHVKLWASSSARDTDFTAML
                     CDVYPDGRSVSILDGAIRACHRNTTREEHLLAPGEVYEFTINLWDTALAFERGHSIRV
                     DISSSNFPRFEANPNTGTPSGSDTTRVVAKNTIYFGAQRPSALILPVIPLE"
     gene            complement(5882..6076)
                     /locus_tag="P5179_08530"
     CDS             complement(5882..6076)
                     /locus_tag="P5179_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95299.1"
                     /translation="MKAKVICVQTTDPGRIGDLLQGKLNEFLAAVPSANVHSTHMAAL
                     DIGGPSQADVLVVYTVFYTE"
     gene            complement(6103..6594)
                     /locus_tag="P5179_08535"
     CDS             complement(6103..6594)
                     /locus_tag="P5179_08535"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRS95300.1"
                     /translation="MDVDPTWRDDGGVRLNVVLVEPEIPQNTGTIARLCAATACALHL
                     VEPLGFDVSDRTLKRAGLDYWQHVTVSVHRSFGALQEAYPGARFWYTSKKAHVPYTQA
                     KFHPDDFIVFGKETTGLPADLLDANSEWSLAIPMFGPVRSLNLANAVSIVLYEALRQL
                     RGF"
     gene            complement(6643..>7078)
                     /locus_tag="P5179_08540"
     CDS             complement(6643..>7078)
                     /locus_tag="P5179_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRS95301.1"
                     /translation="QGSENLVDAYGGVGLFCFALCRDAARVFLIESDASALRDARAIA
                     DAFRAQNVTIVDGSVERAARRIPKADTIICDPPREGAGPGGIRALVSLEPRKLVYVAC
                     DPASLARDSSFLRAEGFRLAWAQPLDMFPHTYHVETVALFER"
BASE COUNT         1411 a         2147 c         2044 g         1476 t
ORIGIN      
        1 acctgggata ccgcgaccag acgagaggat gtccaatcga tggcagttcg tttcggggcg
       61 gcctacgaac aaccattgcc gagaatcaac agcgcggtca ccgtggctgc tgccaagatt
      121 ggcgacggtg gcgcgtgggg tgtggaatac tcgtttgccc ggcagatcat gcttcgggtg
      181 ggagacagcg gaattggaga agggtcattc gccggagggg ccggtttcag gtggagaaac
      241 gttgatctcg attatgcgct tcagcggcac cctctgggca cgagccaccg tgtcagtgtt
      301 caataccatc actagggtgg gactggtcgg gttgcttgtg cccgtggtgg cgcttctggt
      361 gaactggtcg tgcagtccgc agaagtcgaa ttcgcggacg ctcgcgcgac cggtatggga
      421 gtgtccggtt cctccgagtt cggaagtcga agccgggccg agcgccagcc cgctggatga
      481 cttcgtgcac ctcagatgga tcgtcggaag cgatgatgat ctcgacgggt atttcataca
      541 ccggcgcaaa agcactgacc tcatcgacac gcttttcgca accgtgtacc tgactcgcga
      601 ccagttggca aaccgtggct ccaccatcga atgggtggat cgaaattcga gagtggggag
      661 cgtgtactac tacgtgctct tcgcattcga caagaaagga aaccgcagcg ctcgaagtga
      721 taccgtcgga ttcgggaccc tcgaaaaacc catgatttat ggaccatcgc aaaaccaggt
      781 cgtcgttgac agccgtcctg tttttcaatt cggtccgcgc accacggctc tctatgtgca
      841 gagttatgtt gtccgcgtgg tatcggattc ggccaattcg caggttctct gggtaagtcc
      901 gcggaggccg ctgctggggt atacctccgg ggaacgaacg acgattcagt atggcaacgg
      961 cggtttcacc cgccgcccgt tcctcaatcc cgggaagtat cgttggcgtg ttgatttcca
     1021 gggaagttac cccttcgtcg atatagacgc tccctgcggc tgcgtgtacg acacggtgac
     1081 ctgcccgggg gcgagcccca ccctgccacg tccggaggat ctctcgttca tcagctcaca
     1141 atcagcctgg gttccgttca ccgtggcacc ctgacggtct ccagtaatgg tgccttcgat
     1201 tactgctcgc gctcctgcca aactcaacct cggcctttcc atcgtaggac ggcgcgccga
     1261 cgggtaccac gacctgattt cggtttttca tgcggtggac tggcaggaca ctctcgaaat
     1321 tgtggaaatc ccgtccggga ttgaattcgc ctgctcttcg tgcgacggag ttcccggcgc
     1381 gccgcagaac ctcgttttcc gggcgcttga actggtgttc cagagattcc gcatcactcg
     1441 cggcgttgcg gccagactcc agaaagaaat cccggccggt gcgggcctcg gaggcggcag
     1501 ttccgatgct gcggccacgt tgcgagcagt gcgtcgtctc ttcaacctcc agggcgacga
     1561 ggactcctgg ttgttgctct gccagcaact gggctcggac gttccttttt tctggcgggg
     1621 aggaacggct ctggtggaag ggagaggaga acggctcaca cctctctcaa ttgacgagag
     1681 gctctggttc gtggtggtgg taccgccgat tcacatctcg acggcgtggg cgtacggcca
     1741 gctctcaaag ccgtccgggg gtaatcagga ctaccgggac cggatccagc gtcttcgagc
     1801 tggcgagctt tcgctctctg atttctgtcg cgagattccg aacgacttcg aaagagtggt
     1861 cgaagcagcc tttccgcgaa taggggagat acgccgcgcg ctcgaagcct caggggccat
     1921 atccgcgcga atgagtgggt ccgggtcctc cgtgttcggt gtgtattcaa aacaagaaga
     1981 agcacagcgg gccgctgcgc tctttccgtc agattgtctc gtaagatgtg tgcaggggat
     2041 gccctcctcc cgtgatcaaa tgctctgagg gtggtgtctt cggaaacgcc gtaatgtaat
     2101 cggcgtctgg cgcgcaggat gcgttgttcc atgattcaat acgacaatcc caaacctctg
     2161 ctccagcgtt tcaaggtaat tgtgccgtac gtacgcccgt acaccccgta catcctgggt
     2221 gctctcgggt tgacggtggt ccttactgtt ctcgcgttgc tgcctccgct catcatgagg
     2281 gcgctcatcg acagggtcct caccgcgggc aactggcgca tccttctccc gattatcgtc
     2341 gcccacgctc ttgtcccgat catgtccggg ctgatctcct tttttaacac gcttgtgatc
     2401 gcgtacgcgg gtcgaaggct tgtgttcgac gtccgcacgc agatgtaccg ccacctgttg
     2461 cgcctctcaa tgcgattcca cggggaaatg tccagtggcg cggtgatgtc gcgccttatg
     2521 agcgacgtga accaggtgca gaacctcatc agagagaaca gcatcaccct ggtaacagat
     2581 atcgtcagct tcatgttcgc tggattcgtg acgttctcgc tgaactggaa gatggcgatt
     2641 ctactgtgga taatgcttcc cctgtacgtc ttcaactcgc ggttttttgt caaacggatt
     2701 cgccgctcga accagagaat gcggcgtacc atggacaaaa tcgcggggtc gcttcaggaa
     2761 cggctctccg gaacgcgtcg cgtacgggcc tttggccgcg aagaggacga gaccgagcag
     2821 ttcctcgaac aaaccgaaga gaacctcgcc cattcgatgt ccggcactat tcacagtgtg
     2881 tcgttttcca ccgcatcccg ccttgtctgg gggttcggta cgacaattct cttcgtcctc
     2941 ggtgtctatt tcgcgctgca cgggcaaatg acgtacggct ccgttaccgc attcatggca
     3001 tacgtctcgc agatgttcgg ccccgccctc aggttcaccg aactcgcaaa tcagcttgaa
     3061 caggtcatgg tgtcggtcga ccgcatcttc gaggtcctca acaccgaacc cgatgtcgtg
     3121 gaaaaaccgg atgccgttgt tcttacttcg gtggtcggtc acattcagtt cgaccacgtt
     3181 acgttcggat acaaacccga cgaaccagtc atcaaggatg tttgtctcga gattcctccg
     3241 gggaaaatgg tggctcttgt ggggcatacc ggatgcggca agaccaccat caccacactc
     3301 ctcatgcgcc tgtgggacgt ccaggaaggc tctatccgcg ttgatgggca cgatatccgc
     3361 gacgtaacct tcgccagcct gcgaaggagc attggcgttg tgctgcagga tcccgtgcta
     3421 tttaatgcta cgatagccga caacctccgg tacgggtgtc ccgaggcaac aatggaccag
     3481 gtgatcgaag cggcaaaggc cgcggaaatt cacgagatga tcatgaatca gccggagggc
     3541 tacaacacag aactcggggg agagagtggg atcaaaccat ccgtcggcga gaaacagcga
     3601 atggcgatcg cgcgcgctat tatcaccaat ccggcggtgc tgattcttga tgaggccact
     3661 tcggcactcg attcacagag tgaaatgctg atccagaaag cgctctcccg ggtcatgaag
     3721 aaccggacga gcctcgtgat cgcgcaccgc ctgtccacga tagtcgaggc cgacatgatc
     3781 gtggtgatgg atcagggaag ggtgatcgaa acaggttcgc acgccgagtt gttgaaggcg
     3841 ggggggcatt acgcagggct cttcaaccag caacgcggca ccggaaaacc cgccggggtt
     3901 accgccggtt cgtgaggcct ttctgcggcc tccgcgggaa caatacctcg cgaaatgcgc
     3961 gccgcacaga cgcgcaccaa atcccggcga gtagagcaca acggcttctt ttgcttgccg
     4021 cgccaacgcg cagttcccta tctttcatac acccttgtcg gggcgtggcg cagtccggct
     4081 agcgcatctg ctttgggagc agagggtcct gggttcaaat cccggcgccc cgaccaccct
     4141 ctcctgcgtt cgaatccttc gtcccgaatc acctcctctg caccgtcctg cacagttcac
     4201 tcaagtggaa tcaccggcag gataagcgca gaaggccttt gcgcgccgaa gtagatagtg
     4261 ttttttgcca ccacgcgggt cgtgtcggaa cccgacggag tgccggtatt tgggtttgcc
     4321 tcgaaccggg ggaaattgct gctcgatatg tctacccgga tcgaatgacc acgttcaaac
     4381 gcaagcgcgg tgtcccagag gtttatcgtg aattcataga cctctcccgg ggcgagcagg
     4441 tgctcttccc tggtggtgtt ccgatggcaa gcccgaatcg caccgtccag tatcgaaact
     4501 gaccggccgt cggggtacac gtcacagagc attgcagtga aatcggtgtc cctcgcggaa
     4561 gaagacgccc acaactttac gtggacgttg cccgcgatgg cgactggtgc ttccagagga
     4621 ggcgtggtga aactcagcac gtcgggacgc gattccacag accgtacgtc gcgcgggcct
     4681 atcggggggt acagattcag tccacccagc gtgggaaccg ggtttcgcgg atcgaattcg
     4741 tagctcaaag cccctgcttc actggtcggc gcgtcgcggg tcaaagccct gtcctctgcg
     4801 aggtaaaacg cgacgtcctg cgattccggc ggtggccatg tcggtgcatt cagccagcgg
     4861 ttgccagccc cggagctgtc ccccgaagca cccatcaggt agtaccgaac cgccgccacg
     4921 ctgtccaccc gggtgggaac gcctttcagc cggctgtcaa accatgccac cgcatccccc
     4981 atcgggttca cgtccttatt gtttccgggg aactcaatat cgccctgctt cctgcccatt
     5041 ccgtggtgcg accagggccc catcaccatc agttgccttc cgcgcgctcc ctcgccaccg
     5101 cgttcgtgaa gtgcctggaa cccgtttgtc tgccccgcgc tcatcacatc aaaccagccg
     5161 ccgcaatggt acacggccgt gtgtgcgcgg ggagcgtgga ccgaaagatt catggcttcc
     5221 cagtaatcgt catacgacgc gtgctcccgc agcacaggca ggtagtccgc tcggccttgg
     5281 cccgtaaccc atcgctccac cagatctttg cggaacaccc cgccggggaa gaacacgtca
     5341 tcgtagaggt tcgagccgca catcaccacg tacgccgcct taagtgcagg gtgcccgctg
     5401 gcgaccgcca tatacgatgt gatcccaagg gccgacatgc cccacatccc gactgcgccg
     5461 ttgctccagt tctggcgcgt gatccattca atcgtatcgt acccatcctg gttcggcccc
     5521 gcgccgtctg tccggaaaat cgaatcaacg ccggcggagg caaacctgcc ccgtgtatcc
     5581 tgcaccacaa ccgcgtatcc cttgtctgtg aacgtctttg cgatcacgga tgaggtcttg
     5641 atgccattca ccaccgcgcc cttgttgtac gtgttcctga taagaatcgt cggcatccgg
     5701 tgcaggcgcc ccttcgggaa gtaaacgtct gtcgccagtt ctgtcccgtc tcgcattcgc
     5761 accatatgcg tggtggaggg ggggagcctc agaatcaggc tgcaccccga cgcaaaatac
     5821 gccagaaaca ggccgacaag ggccaatttt cgagcgtgca tggcgttact ctgcatccgg
     5881 ttcactccgt gtagaacact gtgtacacca ccagcacgtc tgcctgcgaa gggccgccga
     5941 tgtccagtgc tgccatgtgg gtggaatgca cgttggcgga gggtaccgcc gccagaaact
     6001 cgttcagttt gccttgcagt agatccccga tccgtccggg gtcagtggtt tgtacacaga
     6061 tcactttggc cttcatcgaa gtcctcctga acaacaggcg cttcagaaac cccgcaactg
     6121 ccgcaacgct tcatacagaa caatgctcac tgcgttcgcc agattcaggc tccgcaccgg
     6181 accgaacatc ggaatcgcca gcgaccactc ggaattcgcg tcaaggaggt ccgcgggcag
     6241 acccgtcgtt tcttttccaa acacgataaa atcatcggga tggaacttcg cttgggtgta
     6301 cggaacgtgc gcttttttcg aggtgtacca gaatcgcgca ccagggtatg cctcctgcaa
     6361 cgcaccgaag gatctgtgta cagataccgt cacgtgctgc cagtaatcca gccccgcgcg
     6421 cttcagcgtg cgatcgctca cgtcgaatcc gagcggctcc acaaggtgca gagcgcacgc
     6481 cgtggcggcg cacagccgcg cgatggtgcc cgtattctga ggaatctctg gctcaacgag
     6541 cacaacgttg agtctgactc caccgtcatc gcgccacgtg ggatcaacat ccacgggctc
     6601 cacgaatgga tggggacgtc gacgatcgcc aggcaccgga aatcacctct caaaaagcgc
     6661 taccgtttca acgtgatacg tgtgaggaaa catgtccagc ggctgcgccc atgcaaggcg
     6721 gaatccttcc gcgcgaagga aactggagtc gcgtgccaga ctcgcaggat cacatgcgac
     6781 atacaccagt ttccgcggct cgagagagac aagcgcgcgg attccacccg gtcccgcccc
     6841 ctcgcgaggc ggatcacaga tgatcgtgtc ggccttggga atccggcgtg ccgcgcgttc
     6901 cacggaacca tctacaatag taacattttg ggcacggaag gcatccgcga ttgccctggc
     6961 atcacggagt gccgatgcgt ctgattcgat gagaaaaacc cgcgctgcgt cgcggcacaa
     7021 cgcaaaacaa aaaaggccca cgcctccata cgcgtccaca aggttctcgc ttccctgc
//
LOCUS       DAOTNG010000155         7077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9252_length_7077_cov_5.043150, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000155 DAOTNG010000000
VERSION     DAOTNG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9252_length_7077_cov_5.043150"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="P5179_08545"
     CDS             complement(<1..707)
                     /locus_tag="P5179_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS95302.1"
                     /translation="MPTMSMPWGTMAYTDSGDAGAGSPTMLFLHGTGCDAGDWDAVIE
                     ALPPTVRKLTLEFRGHGRSSVPVGPFTFSDLADDVAQFIEAMKLRRVILVGHSLGGMV
                     GMQVARQGDDTPRHGDVAVLVLLEGWTRLGCMGKAFEGERFYGHLDASAIEAITRKRD
                     ATMARFPAETWEHFWRSVREFDGSAFLSEARIPILEVYGGMGRVPGAEHELRVPENLY
                     IQTMWIPDAGHYLPIEKP"
     gene            complement(828..2165)
                     /locus_tag="P5179_08550"
     CDS             complement(828..2165)
                     /locus_tag="P5179_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS95303.1"
                     /translation="MPHTKSIPYARRLPVRAEVEVLVAGGGPAGVAAAVTAARQGRVV
                     HILEAHSCFGGMGTAGLVPAFMQFTDGVNFLAGGIGKEILDELRRRGGTYPPDGMGIR
                     AEVLKRVYDDMVSDAGVDFTFHAHVVDVLWEERGTVSTVVCAGKTGLFAVEAKTFVDC
                     TGDGDVCAFAGAPYEKGDETGAMMPGTLCSLWADIDWEAVKAGGITGEERNKRLEQAL
                     KDGVFTLEDRHLPGIWPVGDHVGGGNVGHTFEVDGTDEESLTAAYVWGRKSMTEYERF
                     YKEYLKGYEKMALVATGSLLGVRETRRIMGDYVLTVDDYTNRAVFEDEIGRYSYPVDI
                     HIAKPDRKSYDAFMKEHTTLRLGKGESYGIPYRILTPKGLKNVLVAGRCVSTDRQAQS
                     SIRVMPGCYITGQAAGMAAALMVETGKDSRGIEVRELQKRLKAMGGYLPNFAG"
     gene            complement(2175..2345)
                     /locus_tag="P5179_08555"
     CDS             complement(2175..2345)
                     /locus_tag="P5179_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRS95304.1"
                     /translation="MRKELLDILVCPKCKGDLDYSEAEQTLTCRACRLRYEIKNGIPI
                     MLVDKAIPIPSE"
     gene            complement(2342..3364)
                     /locus_tag="P5179_08560"
     CDS             complement(2342..3364)
                     /locus_tag="P5179_08560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRS95305.1"
                     /translation="MNAATQGLPHGPRPAPLDFEAELVKLEDRITDMRKWADEYSKTG
                     QDPAIAQEIAKLEARANEMRAAIYRNLTHWQRVQVARHRDRPYTLDYINLLMEDFSEL
                     HGDRHFADDAAMVCGIARFRGRSVALVGQQKGRTVEERIKRNWGMAGPEGYRKALRIM
                     KLAEKFRLPVISLVDTPGAAPGVGAEERGQAEAIARNLFEMSRLQTPILVAIIGEGAS
                     GGALGIGVGDRVLMMENCWYSVISPEGCAQILWRTADAKEQAAAAMKISAQDLHALGL
                     IDEVVPEPVGGAHRAPEEAARILGDRLEENLNLLAAIPPTELLERRRNRLYAYGQWEE
                     REVASR"
     gene            3704..5698
                     /locus_tag="P5179_08565"
     CDS             3704..5698
                     /locus_tag="P5179_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS95306.1"
                     /translation="MHATLLSDILIIFALSVAVVFICERLHVPSLVGFFLTGIVAGPH
                     ALGLVSDVARVESISEVGIVALLFTVGLSFSLEHALRVRRTVLVGGTIQIVATGAAGY
                     LAARALGQPPPVAVLAGFLLSLSSTAIAIKSLQDRAEADSLHGRIVIGILVFQDIITV
                     PMLFSLPLLAEATAQSAGSLLTMAAQGLGVIAIVIVSARWVVPYVLYQIARTRSGELF
                     LFAVVVICLAITWLTYSIGLAPALGAFLAGIIISESEYSQQALGDVIPFRVIFSNLFF
                     VSVGMLLDIGFLFDHAIAVAAMACGSIIIKVLLAGAATALLGYSLRTTIGVALGLGQI
                     GEFSFILAASGLQFGLLEPSGYQYFLTVSVITMAATPFLIQAAPHIADHLLRLPLPKR
                     VRAGSFAREDDGNAGLTDHLIIIGYGVNGRNVASAAAEALIPYVIIEMNPETVRRERA
                     NGESILFGDGSQPVVLEHAGILQARVLVVATPDATGTRRIVENARRMNPSLYIIARTR
                     FVTEVAPLYKLGADEVIPEEFETSVEIFSRVLRKYLIPREQIENFIRTIRAGGYDMFR
                     SFSGDFTALSEIQTSLRDVEVSTFPVDEGCRMAGQTLAELGLRSRTGITILGIRRGDK
                     TIPNPGGEETIHAGDVLLVMGPPDAVASVSDLLKSECAIL"
     gene            5749..>7077
                     /locus_tag="P5179_08570"
     CDS             5749..>7077
                     /locus_tag="P5179_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95307.1"
                     /translation="MSPLLGMYVHMHWGYAYPYAARTWSLEDWRAYASGLRALGYNLV
                     MIWPLTETMPDPLTASDVAHLEKLGNVITMLQHEFGMTVLITFGANTIGNTHAAEYPF
                     ETRPFFRTDLRLNPADPSAMDRLMRFRASLYRYLAHADGIVVIDSDPGGYAGSTNAEF
                     TAILRRHLKLLRDANPEAMLWYWLWVGWESYNRFWEQAEKGGDAKLEVNPGDWAEVVE
                     DLCTTPDDPWRLLVCWDSHRQLAEHLGIEDRCVYFPYGAVEGEPTVPLTNCDPAGVEK
                     ALSGYPWGRPYLGCMANAQTHIVQLPNTYLFAHFARGGRLEDVDLAGFAEKLIPGQGS
                     LIAEAWKALADRAIEPARRLAKELTRGEARQLPRGEFSGLLMHDPTRFLGDLVLQLAF
                     SADLSDFAAAVDAGGDGTREMRGLVASWRAWTERTRFVDAYGGPVMDRLHP"
BASE COUNT         1218 a         2411 c         2053 g         1395 t
ORIGIN      
        1 ggcttctcga tggggagata atgccccgcg tcgggtatcc acatggtttg aatatagaga
       61 ttctccggca cccgcagctc gtgttccgcc cccggcacgc ggcccatccc gccgtatacc
      121 tccagaatcg ggatacgtgc ttcgctcagg aaggcggacc cgtcaaactc acgcacggat
      181 cgccagaaat gctcccacgt ctctgcgggg aaccgggcca tcgtcgcgtc gcgtttccgc
      241 gttatcgctt cgatggcgga agcatcaagg tggccgtaga agcgctctcc ctcaaacgcc
      301 ttccccatgc accccagccg cgtccagcct tccagcagca caagtacggc gacgtccccg
      361 tggcgcgggg tatcgtcgcc ctgccgtgca acctgcattc ccaccatgcc gccaaggctg
      421 tgccccacca gaatcacccg ccggagcttc atcgcctcga tgaactgcgc cacgtcgtcc
      481 gcaaggtcgc tgaacgtgaa cggtcccacc ggcacactgc tccgcccgtg cccgcggaat
      541 tccagcgtca gcttccgcac cgtgggtgga agcgcctcga tcacggcatc ccagtccccg
      601 gcatcgcacc cggttccgtg aagaaacaac atggtcggcg acccggctcc cgcgtctccg
      661 gagtccgtgt acgccatggt tccccacggc atgctcatgg ttggcatggg cggtttcccg
      721 ctaatgtgag ccttttcgtc attctgaacg cagtgaagaa tctctatccc gggaacagtc
      781 acgacgactg caaaacggac gcgaggaaac gcgtccctcc aacagggtta ccccgcgaaa
      841 ttcggcagat atccgcccat cgctttcagg cgcttctgca gttcccgcac ctcgatgccg
      901 cgggaatctt tccccgtctc caccatcagc gcggccgcca tccccgctgc ctgtccggtg
      961 atgtaacacc ccggcatcac ccggatggag ctctgcgcct gcctgtccgt gctcacgcac
     1021 cgcccggcca ccaggacgtt tttcagtcct ttcggcgtga ggatgcggta cggaataccg
     1081 tagctctccc ctttcccgag gcggagcgtc gtgtgctctt tcatgaaggc gtcgtagctt
     1141 ttcctgtccg gctttgcgat atggatatcc accggatatg agtaacgacc gatttcgtcc
     1201 tcaaaaactg ctctgttcgt gtaatcatcc acggtcagca cgtagtcacc catgatgcgc
     1261 cgcgtctcgc gcacacccag cagcgacccc gtagctacga gtgccatctt ctcgtagcct
     1321 ttcaggtact ccttgtagaa ccgctcgtat tcggtcatgg atttccggcc ccagacgtac
     1381 gccgccgtga gcgactcctc gtccgttccg tccacctcaa acgtgtgccc gacattgccg
     1441 ccaccgacat gatcgcccac cggccatatg ccgggaagat ggcgatcttc gagggtgaac
     1501 acaccatcct tgagcgcctg ttcgagccgt ttgttgcgtt cttccccggt gatgccgccc
     1561 gccttcaccg cttcccagtc aatatcggcc cagagactgc acagcgtgcc cggcatcatc
     1621 gccccagttt cgtcgccctt ttcgtacggc gcgccggcaa acgcacacac gtcgccatca
     1681 ccggtgcaat ccacgaacgt tttcgcttcc accgcgaaca gacccgtttt tcccgcacac
     1741 acgacggtgg aaaccgtacc ccgctcctcc cacagcacgt ccaccacgtg tgcatggaag
     1801 gtgaaatcca cgcccgcgtc gctcaccatg tcatcataca cgcgcttcag cacttccgcc
     1861 cggataccca ttccgtcagg cggataggtt cctccgcggc gccgcagctc gtcgaggatc
     1921 tctttcccga tgccgccggc gagaaagttc acgccgtcgg tgaactgcat gaacgccggc
     1981 acaagacccg cggtacccat gcctccaaag caggaatgtg cctcaaggat atgcaccacg
     2041 cgcccctgac gcgctgccgt gacggccgcc gccacgccgg cagggccacc acccgcaacc
     2101 agcacctcaa cctccgcacg gacgggaagc cgtctggcgt acggaatgga ctttgtgtgt
     2161 ggcatggaag cctcctattc cgaggggatc gggatcgcct tgtccaccag catgatcgga
     2221 atgccgtttt tgatctcgta ccgaaggcgg catgcccggc acgtaagcgt ctgttccgcc
     2281 tcagaatagt cgaggtcgcc tttgcatttg gggcacacaa ggatatcgag cagctctttc
     2341 ctcatcgcga cgccacctcc ctttcttccc actgaccgta ggcgtacagc cggttgcgcc
     2401 ggcgctcaag cagctccgtt ggcggaatgg ctgccagcag gttcaggttc tcctccagcc
     2461 ggtcgccgag gattcgcgcg gcctcttccg gtgcacggtg cgcgccgccg accggctcgg
     2521 gcaccacctc atcaatcagt cccagcgcgt gcaggtcctg cgcgctgatc ttcatcgcgg
     2581 cggcggcctg ttccttcgcg tcggccgtgc gccagaggat ctgagcgcac ccctccgggc
     2641 tgatgaccga ataccagcag ttctccatca tgagcactcg atctccgacg ccgattccga
     2701 gcgctccccc ggaggcacct tctccgatga tggcgacaag tatcggcgtc tgcagacgcg
     2761 acatctcgaa caggttccgc gcaattgcct cggcctgacc gcgttcttcc gccccgacgc
     2821 ccggcgccgc cccgggagta tctaccagcg atatgaccgg cagacggaac ttctccgcca
     2881 gtttcatgat gcgcagcgcc ttgcggtacc cctccggacc cgccatgccc cagttgcgct
     2941 tgatcctctc ctcgaccgta cgtcccttct gctgccccac gagggcaacg gaccgcccgc
     3001 ggaaccgggc aatgccgcat accatcgctg cgtcgtccgc aaagtgccgg tccccgtgca
     3061 gctcgctgaa gtcttccatc agcaggttga tgtagtcgag cgtgtacggc cggtcgcgat
     3121 ggcgggcgac ctgcacccgc tgccagtgcg tcagattgcg gtaaatcgcc gcgcgcatct
     3181 cattcgcgcg agcctccagc ttggcgattt cctgcgcaat ggccggatcc tgccccgttt
     3241 tcgagtattc gtcggcccat tttctcatgt cggtgatccg atcttcaagc ttcacgagtt
     3301 ccgcctcaaa atccagcggc gcgggacggg gtccgtgcgg cagaccctgc gttgcggcgt
     3361 tcatgcgcgc tccttgtgtg gcaatcatcg cgtgattccg gctggtcccc cgaacgcgtg
     3421 accacacacc ggtggaataa agagtagaca agcccgcacg ggatgcaaag gcgcgggcga
     3481 aagcgccggc ccgccctgcc ctttgatcct ggagtgcacg ccctttttca ggtgaggtaa
     3541 aacggcgtgt tcgtgtcaag ggggtaagca gtttttttcc gaacagcaac ggagcgcgtc
     3601 cgacccgcgc cggatcgccg cgcaacttgc ccggcggggc ccggaatcgt aatttccccc
     3661 tgcgtgttcc cgcagcatcc aactgcgaca cgaaaggccg aatgtgcacg caacgctcct
     3721 gagtgacatc ctcatcatct tcgcgttgtc cgtcgccgtg gtgtttatct gcgagcgcct
     3781 gcacgttccc tctctcgtcg gcttcttcct caccggcatc gtggccggac cgcacgcgct
     3841 ggggctggtg agcgatgttg cgcgagtgga aagcatttcc gaagtcggca tcgtcgccct
     3901 gttgttcacc gtcgggctga gtttctcgct ggagcacgcg ctcagagtca ggcgcaccgt
     3961 gctggtcggc ggaacaatcc agatcgtcgc gacgggcgct gcggggtacc tggcggcccg
     4021 cgcgctgggt cagccgcccc cggtcgccgt tcttgccggt tttctcctct cgctcagcag
     4081 cacggcgatt gccatcaaaa gcctgcagga cagggcggaa gcagattccc tccacggccg
     4141 catcgtcatc ggcattctcg tgtttcagga catcattacc gtcccgatgc tcttttccct
     4201 cccgctcctt gcggaagcga cggcccaatc cgccgggtcg ctcctcacca tggcggccca
     4261 gggcttgggg gtaattgcca tcgtcatcgt gagcgcccga tgggtcgtgc cgtatgtgct
     4321 ctaccagata gcgcgaacgc gaagcggcga gttgttcctg ttcgccgtgg tggtcatctg
     4381 cctcgccatc acgtggttga cgtactccat cggactggcg cccgcgctcg gcgcgtttct
     4441 cgccggtatc atcatctcgg agtcggaata cagccagcag gcactgggcg acgtcatacc
     4501 gtttcgcgtc atcttttcga acctgttttt cgtctccgtc gggatgctcc tcgatatcgg
     4561 gttcttgttc gaccacgcaa tcgccgttgc cgcgatggca tgtggttcca tcatcatcaa
     4621 ggtgcttctt gccggggccg ccaccgcgct cctcggctat tccctccgca cgacgatcgg
     4681 ggtggccctg ggcctcggcc agatcggaga gttctccttc attcttgctg cgtccgggct
     4741 tcaattcggg ctgctcgagc cctccggtta tcagtatttc ctgaccgtgt cagtgattac
     4801 gatggctgca acgcccttcc tcatacaggc agcgccgcat atcgcggatc atctcctccg
     4861 cctgcctttg ccgaagcggg tgcgagccgg atcattcgcg cgagaggacg acggaaacgc
     4921 aggcctcacc gaccatctga tcatcatcgg atacggcgtg aacgggcgga acgtcgccag
     4981 tgcggccgcc gaggcgctga ttccctacgt tatcatcgag atgaatccgg aaaccgtccg
     5041 gcgtgaacgg gcaaacggag agtccatcct tttcggcgat ggctcgcagc cggtcgtgct
     5101 agaacacgcg ggcatcctgc aggcgcgggt gctcgtggtc gcgacgccgg acgcaaccgg
     5161 cacgcgccgg atcgtcgaaa acgcacgccg gatgaacccg tccctttaca tcatcgcccg
     5221 tacgcggttt gttactgaag tcgcgccgct gtataaactt ggagccgacg aagtgattcc
     5281 ggaagaattc gaaacgtcgg tagaaatctt ctcccgcgtt ctgcgcaaat acctgattcc
     5341 ccgggagcag atcgagaact tcatccggac aatccgcgcc ggcgggtacg acatgttccg
     5401 gagcttctct ggcgatttta ccgcgctctc agaaatccag acttctttgc gcgatgtgga
     5461 agtcagcacg ttccccgtgg acgaagggtg ccggatggca gggcagacac tcgccgaact
     5521 gggtctgcga agtcgcaccg gaatcactat tctcggcatc cggcgcgggg acaagaccat
     5581 cccgaacccc gggggcgagg agacgattca cgcaggcgac gttcttcttg tcatgggtcc
     5641 gccggatgcc gtggcctccg tctccgacct tctcaaatcg gaatgcgcga tcctttgacc
     5701 cggctctccc gtgccccgga tggcgggagg cagaaccccg gagtgaagat gagcccgctt
     5761 ctcggcatgt acgtgcacat gcactggggt tatgcctacc cgtatgccgc gcggacgtgg
     5821 tcgcttgagg actggcgcgc ctacgcctcc gggctccgcg cgctcggtta taacctcgtc
     5881 atgatctggc cgcttacgga gacaatgccg gatcccctga cggcgagcga cgttgcccat
     5941 ctggagaaac tcggcaacgt aatcaccatg ctccagcacg agttcggcat gacggtgctc
     6001 atcacgttcg gcgcgaacac aatcgggaac acgcacgccg cggagtatcc attcgaaacg
     6061 cgcccgttct tcaggaccga cctgcgcctg aaccctgccg atccttccgc catggacagg
     6121 ctcatgcggt tccgggcctc gctgtaccgc taccttgcgc atgcggacgg catcgtcgtc
     6181 attgatagcg acccgggcgg gtatgccggc tcaacgaacg ccgaattcac cgccatcctg
     6241 cgcaggcacc tcaagctgct ccgcgacgcg aacccggagg cgatgctctg gtactggctc
     6301 tgggttgggt gggagagcta caaccggttc tgggaacagg cggaaaaggg cggggacgca
     6361 aagctggaag tcaacccggg cgactgggcg gaggtggtgg aagacctctg caccacgcct
     6421 gacgacccgt ggcgccttct tgtctgctgg gacagccacc ggcagcttgc cgaacacctg
     6481 ggcatcgaag accgatgtgt ctacttcccc tacggcgcgg tcgaaggaga gccgaccgtt
     6541 ccactgacaa actgcgatcc cgctggcgtc gaaaaagcgc tctcgggcta cccgtggggc
     6601 cggccgtacc tcggctgcat ggccaatgcg cagacgcaca tcgtccaact ccccaacacc
     6661 tacctgttcg cccacttcgc ccggggcggc agactcgaag acgtcgatct ggcgggcttt
     6721 gcggagaagc tgattcccgg tcagggcagc ctcatcgcgg aggcgtggaa ggcactggcg
     6781 gatcgcgcga tcgagcctgc gcgtcgtctg gcgaaggaac tcacccgcgg ggaggcacga
     6841 caactgcccc gcggcgagtt ctcagggctg ctgatgcacg atcccacgcg cttccttggc
     6901 gatcttgtcc tgcaactggc gttttctgcc gatctgagcg acttcgcggc ggcggttgac
     6961 gcgggcggcg acggcacacg cgagatgcgc ggtctggtcg cgtcgtggcg tgcgtggacg
     7021 gagcgcacca ggtttgtgga cgcctacgga ggccctgtga tggatcgcct ccacccg
//
LOCUS       DAOTNG010000156         7071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9261_length_7071_cov_5.491163, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000156 DAOTNG010000000
VERSION     DAOTNG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9261_length_7071_cov_5.491163"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..714)
                     /locus_tag="P5179_08575"
     CDS             complement(178..714)
                     /locus_tag="P5179_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95308.1"
                     /translation="MVRELKQAYLPSEKVVTMDASGVIIRPYEASDIKIIKEITLEAF
                     ERVSIDKNIEDRFGKLGGTDWRERKARHIDDDIAANPEGILVAEYQGQVVGYVTITLD
                     ARTRLGRIPNLAVKAGLRGLGIGSKLIEAACEYMKSRGMTHGKIETLDQNEVGLHLYP
                     KMGFEEVARQVHFVKKLQ"
     gene            complement(699..1505)
                     /locus_tag="P5179_08580"
     CDS             complement(699..1505)
                     /locus_tag="P5179_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95309.1"
                     /translation="MAKRPVGCAIGNLRNGPAAERLGAIRFFGVECIEPQFDIDAFDA
                     DDEPFMHAARDQFKQARLSVWSVHAPFGLEVDLSSADTAIRARGTAAVKRAARGAAFL
                     GAGVMVVHCGDLCGSDQDHQGPLERSREALSEILTACDGINVRIALENLPPGRLTCEA
                     EELMSVVTAFPADRVGVCLDTGHANIAGTLNSLMDAVSSRIITVHLHDNDGSEDQHLV
                     PGDGTIDWQMVRSALDSSPYDGPLMFEVDRPGAYAEMMVDVMKTAEWFAN"
     gene            complement(1520..2899)
                     /locus_tag="P5179_08585"
     CDS             complement(1520..2899)
                     /locus_tag="P5179_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRS95310.1"
                     /translation="MNPNIFREYDIRGLHETDLTSDLTMQIGRALGTELIRRGESSLC
                     VGRDVRPSGVRLREALVEGLLSTGICVRDLGVVPTPALYFGILHLKTGGGVQITGSHN
                     PPEFNGFKMCHGLSAIYGEQIQGLRRLIETSDFERGTGQYSEEDIRSAYTSDILQRTA
                     ISRRLRVVIDAGNGCASEIAPAIFRSLGCEVTPLFCEVDGTFPNHLPDPTMARYMHDL
                     ISKVKETGADIGIGYDGDADRIGVVDDKGRIVEGDRLLALFARDVLTRVPGAEIIFDV
                     KCSQALPEDIARHGGKPVMWKTGHSLIKAKMKETGAPLAGEMSGHIFFSEGFHGYDDA
                     IYCSARLAGILAASGEPMSALADAVPRFVSTPEIRVPATDEQKFAVVSELAEYFKRDH
                     EVIDIDGARVLFGDGWGLVRASNTQPVIVMRFEAKTKNRLKEIADTILTELRRHPSVD
                     LSDVSLDAT"
     gene            complement(2923..3669)
                     /locus_tag="P5179_08590"
     CDS             complement(2923..3669)
                     /locus_tag="P5179_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS95311.1"
                     /translation="MAKTLRIAGIQMAVSADLDTNLPKILHNLSIAAEEGAQFVLFPE
                     MSLSGYHGNYDFEKVKGAVETIRTALRRLRVGAIISTGWREGGEAFIQQRAYDECGDL
                     LGTHEKMIPTGIDGESGDRAFCVPGKELRTFQWNGITCGMLICNDMWATPGCGPHKDL
                     RLMYQLGQKGTRIVFHSINSGAGARHTPYHESNISLRAEESHMYVCTANAARPDRGIN
                     CSSGVVAPDGQWVTRVHRVGEGRYHAVLTL"
     gene            complement(3789..4283)
                     /locus_tag="P5179_08595"
     CDS             complement(3789..4283)
                     /locus_tag="P5179_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS95312.1"
                     /translation="MNPTRFLEHVDRTYKPAPALLSLIPVERLDWAPATNMMTIRQLI
                     HHLSARTGVHGLVTGDFDPSRCWGDNDDPSSITPGIAKERLAKAVEEVKRSLSEVSIE
                     DWEHRIVEMPWSVKGTVETMAYGLIIEHFLNHKMQLFLYLKLLGIPVDTGTLYFGLLP
                     GQKH"
     gene            4433..5074
                     /locus_tag="P5179_08600"
     CDS             4433..5074
                     /locus_tag="P5179_08600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRS95313.1"
                     /translation="MGEFTFWLHQLAAHHPAEAYAILFLSAVVENLFPPVPGDTVTVF
                     GAYLVGRGSLELWPVIWATVAGSASGFLGLYVLGRTYGRGVLSRLRWVQRSMPRIALA
                     ERLCRRRGILVVLANRFLPGLRSVVSITVGLLRMPAWQVVPAAVFSIFAWNGLLIWGG
                     LLAGENWERAVEIVSRYNTYFGALLVVMALGVSAWWWRTRRHRRPILHGENPR"
     gene            5071..6522
                     /gene="cysS"
                     /locus_tag="P5179_08605"
     CDS             5071..6522
                     /gene="cysS"
                     /locus_tag="P5179_08605"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRS95314.1"
                     /translation="MSLQVYNSLTRTKEIFEPIVPGRVGIYVCGPTVYGDSHLGHAKA
                     YISFDVIVRYLRFLGYKVRYVQNITDVGHMTDNDEDQGEDKIAKKAALERIEPMEVVT
                     HYMMSYFDDMAALNVARPDIICLASGHIPEQIALIQRLLDAGVAYVSNGSVYFSVDQF
                     RSYGKLSGRRVEELEEGARVAVNLEKKNPADFALWKKAEPGHLMVWDSPWGKGFPGWH
                     LECSAMSMKYLGETFDIHGGGLENQFPHHECEIAQSEAATGKPFVRYWLHNNMLTING
                     QKMGKSLGNFVTIKEMLQKISPEALRLFILMSHYRSVTDFSDEALNSAEKGMARLSAA
                     VQALSQRLPGAAEGPADRKTDAELAEIRRKFVVAMDDDFNTAEAIAQLFSVVRLTNSV
                     LSAPNPATRGTLELLNTQFDELGEGVLGLRLKARAEGSEDLTDQIMQVLIQIREKLRK
                     EKQYAMADEIRQMLAEVGIVLKDEKGRTTWARS"
     gene            complement(6532..>7071)
                     /locus_tag="P5179_08610"
     CDS             complement(6532..>7071)
                     /locus_tag="P5179_08610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS95315.1"
                     /translation="DVVTEAGRLGMEVHSDIQSLLNQAKFQRESFREIERALANNIEF
                     RDHLPSIPPVDLAQVYVSSVFGYRADPYTGRRRIHKGIDLAGRTGTSVVSTADGVVAD
                     VSWGGYTGLVVQIDHGYGYSTVYGHLAKALVREGQRVARGQTIAELGNTGRSTGPHLH
                     YSVFYHGRAVDPANFFYVQ"
BASE COUNT         1451 a         2062 c         1997 g         1561 t
ORIGIN      
        1 aatcaggatc gagccagaac tgccggacat ccttcggcgg aggtggcggc tgcaaactgg
       61 tcggccgagg agcacgcaca agaatcgtgg ccctgtctac atggcttctg ttccaccacg
      121 cgatcaggtg cgttttcgcc tcatcccagt cgggtttgta cagcaacttt gcctccgcta
      181 ttgaagcttc ttcacgaaat gtacctgtcg tgctacctct tcgaagccca ttttggggta
      241 gagatgaagc ccaacttcgt tctggtccag cgtttcgatc ttaccgtgcg tcatgccgcg
      301 agatttcatg tactcgcagg cggcttcgat gagcttgctt ccgatgccga gcccacgcag
      361 ccccgctttc accgcaaggt tggggatcct tccgagacgc gttcgtgcgt cgagcgtaat
      421 cgtgacataa cccactacct ggccctggta ttctgcgaca agaatcccct ccgggttcgc
      481 ggcgatgtca tcgtcgatgt gcctggcttt gcgctcacgc cagtcggtgc cgcccagctt
      541 gccgaaacgg tcttcgatgt ttttgtctat cgagacccgt tcgaaggctt ccagcgtgat
      601 ctccttgatg atcttgatat cactggcttc gtaggggcgg atgatgaccc cggacgcgtc
      661 catagtcact actttctccg agggaaggta tgcctgtttc agttcgcgaa ccactccgcc
      721 gttttcatca catctaccat catctcggcg tacgcacccg ggcgatcaac ctcgaacatg
      781 agcggcccgt cgtacggtga ggaatccagc gccgagcgca ccatctgcca gtcaatcgtt
      841 ccgtcacctg gaacaaggtg ttgatcttcg cttccgtcgt tatcatgcag atggaccgtt
      901 atgatccgtg acgacacggc atccatgagg gaattcaacg tgcctgcgat gttggcgtgg
      961 ccggtatcga ggcacacgcc aacacggtcg gccggaaatg cggttaccac agacatcagc
     1021 tcttcggctt cgcacgtgag acggcctggc ggaaggttct ccaacgcgat ccgaacgttg
     1081 atcccgtcac atgccgtcag aatctcagac agcgcctccc tggacctttc aagcggcccc
     1141 tgatgatcct gatcgctgcc acaaagatca ccgcagtgta ccaccatcac gcccgctccc
     1201 agaaaggccg caccgcgtgc agcccttttg acggcggcgg tgccacgcgc tcgtatcgcg
     1261 gtgtcggcag atgacaagtc cacctcaagc ccgaagggtg catgaacgct ccacaccgaa
     1321 agtcgagcct gcttgaactg gtctcttgcc gcgtgcatga atggctcatc atcggcgtcg
     1381 aaggcatcaa tgtcgaactg tggctcgatg cactccaccc cgaaaaacct gattgctccc
     1441 aggcgttctg cagcgggccc gttacgcaga tttcctattg cgcatcccac cggcctcttt
     1501 gccacactac cctccggatt cacgttgcgt cgagtgaaac atcggacaga tcgaccgacg
     1561 gatgccgtct cagctcagtg aggatcgtgt ccgcgatctc tttcagccgg ttcttcgttt
     1621 tcgcttcaaa acgcatgacg ataaccggct gagtgttgga agcgcgcaca agtccccaac
     1681 catcgccaaa aagaacgcgc gcgccgtcga tatctatgac ttcgtggtcg cgtttgaaat
     1741 actctgcgag ctcagacacc actgcgaatt tctgttcgtc agtcgccggt accctgattt
     1801 ctggcgtgct cacgaaacgg ggcactgcgt cggcgagggc ggacatgggt tccccgcttg
     1861 ctgcaaggat gccggccagc cttgcggagc agtagattgc atcatcatag ccgtggaagc
     1921 cttcactgaa gaagatgtgg ccggacattt ccccggcaag aggtgccccg gtctctttca
     1981 tcttcgcttt gataagcgaa tggcccgttt tccacatcac cggtttcccg ccgtgccggg
     2041 cgatatcttc aggcaatgcc tgcgagcatt tcacgtcgaa aatgatctcg gctcccggta
     2101 ccctcgtaag aacgtcgcgc gcaaaaagag cgaggagcct gtctccctcc actatccggc
     2161 ctttgtcgtc aaccacaccg atccggtctg catcgccgtc gtatccgata ccgatgtctg
     2221 caccggtttc tttcactttg gagatgaggt cgtgcatgta cctggccatt gtcgggtccg
     2281 gcagatggtt gggaaacgtg ccgtcaacct cgcaaaaaag tggggtaact tcacatccca
     2341 agctgcgaaa aatcgcgggg gcaatctcac ttgcgcatcc atttccggcg tcgatgacca
     2401 cgcggaggcg ccgcgatatc gccgtgcgct gaaggatatc agatgtgtag gcggagcgga
     2461 tgtcctcttc gctgtactgc ccggtgcccc tctcgaaatc cgatgtttca atcaaacggc
     2521 ggagaccctg aatctgctcc ccatagatcg ccgaaagacc gtggcacatt ttgaacccgt
     2581 tgaactccgg cgggttgtga cttcccgtga tctggacgcc gccgcctgtc ttcagatgga
     2641 gaatgccgaa atagagggca ggggtcggca cgacaccgag atcgcgcaca catattcccg
     2701 tgctcagcaa tccttccacg agcgcttcgc gcaacctcac gccggagggg cgcacgtcac
     2761 gcccgacaca gagcgacgat tcgccacgcc tgatcagctc cgtcccaagc gctcggccga
     2821 tctgcattgt aaggtcactg gtcagatcgg tttcgtgaag cccgcggata tcgtactcac
     2881 ggaatatgtt ggggttcatt cgcacagccc ttctgggttt tgtcacaggg taagcaccgc
     2941 gtggtaacgc ccctctccca cgcggtgcac cctggtgacc cattgcccgt cgggcgccac
     3001 cacgccgctg gagcagttga tgccgcgatc cgggcgagcg gcgtttgccg tgcacacata
     3061 catgtggctt tcctctgcgc gcagggagat attggactca tggtaaggcg tatgccgggc
     3121 gccggcaccc gaattgatgc tgtggaacac gattctcgtc cccttctggc ccaactggta
     3181 catgagccgg aggtccttgt gaggcccaca acccggtgta gcccacatgt cgttacagat
     3241 gagcattccg caggtaatcc cgttccattg aaacgtgcgg agttctttgc ccggaacgca
     3301 gaacgcccgg tcccctgatt caccgtcaat acccgttggg atcatcttct cgtgagttcc
     3361 cagcagatct ccgcactcat cgtacgcccg ctgctggatg aaggcctccc ctccttctct
     3421 ccaccccgtc gaaatgattg cccccacacg cagtctgcgc agcgcggtgc ggatcgtctc
     3481 cactgcgccc ttcaccttct cgaagtcgta gttcccgtga tagccgctga gcgacatctc
     3541 gggaaacaac acgaactgcg ccccctcctc ggcggcgatc gacaggttgt ggaggatttt
     3601 cggaagattg gtgtcaagat cggcggatac cgccatttga attcccgcga ttctgagcgt
     3661 tttcgccatt catcaaccct tcaaggcaga ctgacttttc cttcgcgcga actgaaaaac
     3721 aagaatagag gggtgccccc gattttgcca aacatcatcc gtcatgcgtt cgatcggcgt
     3781 gcgaccggtc agtgcttctg ccccggaagc agtccaaaat agagcgtgcc cgtatcgacc
     3841 gggatgccca gcaatttcag gtacagaaaa agctgcattt tgtggttcaa gaaatgctct
     3901 attatcaacc cgtacgccat cgtttcgaca gtccctttca cactccacgg catctcgact
     3961 attcggtgct cccagtcttc gatactgact tcggataaag accttttgac ctcctcgacg
     4021 gctttggcca accgttcctt ggctatgccc ggcgtgatag aagaggggtc atcgttgtcg
     4081 ccccagcacc tggaagggtc gaagtcaccg gtcaccaatc cgtgcacgcc ggtgcgcgcc
     4141 gaaagatgat gaatcaactg ccggatcgtc atcatgttgg tcgcgggtgc ccaatccaac
     4201 cgttcgactg gaatcagact caaaagtgcg ggggccggtt tgtaggtcct gtccacatgc
     4261 tcgagaaaac gcgtcgggtt catcggtgtc tcctttgtct ggaggtgttt gaggaggggt
     4321 aaactgaagc acgcattcgc gaaggagtgt gtcaaggcga cccgcccagt agttaccttt
     4381 gttaacggaa cgcaccacgg aatgaccaag attcgaaaca ggagccggtt gtttgggcga
     4441 attcactttc tggctgcatc aacttgcggc gcatcatccc gcagaagcgt atgccatcct
     4501 gtttctcagt gcagtggttg agaatctttt ccctccggta ccgggtgata cggtgacggt
     4561 tttcggcgca tacctggtag gtcgcggctc gctcgaactg tggccggtca tctgggcaac
     4621 ggtggcgggg agcgcctcgg ggtttctcgg cctttacgtg ctcgggcgca cgtacgggcg
     4681 cggcgtgctc tcacgcctcc ggtgggtaca acgttcgatg ccacgaatcg cgctggcaga
     4741 acgactatgt cgccggcgcg gtattcttgt ggtcctggcg aatcgatttc ttcccggcct
     4801 tcgttcggtt gtctcgatca ccgtcgggtt gcttcgaatg cccgcgtggc aggtggtccc
     4861 ggctgcagtg ttcagcatat tcgcctggaa cggccttctc atctgggggg ggcttcttgc
     4921 gggcgaaaac tgggaacgag cggtggagat cgtcagccgt tacaacacct atttcggtgc
     4981 attgctcgtg gttatggcgc ttggtgtatc cgcgtggtgg tggagaacgc gccgccatcg
     5041 gcgtccaatt ctgcatggag agaatccgcg atgagtcttc aggtttacaa cagcctcacc
     5101 aggaccaagg aaatcttcga gccgatagta cccggcaggg tcggcattta tgtgtgtgga
     5161 ccgactgtct acggtgactc ccatctgggg cacgcgaagg cttacatctc atttgatgtg
     5221 attgtgaggt acctgcgttt tctggggtac aaggtccggt acgtccagaa tattaccgat
     5281 gtgggacaca tgacggacaa tgacgaggac cagggcgagg acaagatcgc caaaaaggca
     5341 gccctggaac ggatcgaacc gatggaagtc gttacccatt acatgatgag ctatttcgat
     5401 gacatggccg cgctcaacgt tgcgcgtccg gacataatct gccttgcttc aggtcacatt
     5461 ccagagcaga tcgcacttat ccaacgactt ctcgacgccg gggtggcata cgtctcaaac
     5521 ggatccgtct atttttcggt cgatcagttc cgcagctatg ggaaactttc ggggcggcgc
     5581 gtggaagagc ttgaggaagg ggcccgggtg gctgtcaacc tcgagaaaaa gaacccggcc
     5641 gattttgcgc tctggaaaaa ggcggaaccg ggtcatctga tggtatggga tagcccctgg
     5701 ggcaagggtt ttccgggatg gcatctcgag tgttccgcca tgtcgatgaa gtacctcggt
     5761 gagacattcg acatccacgg aggtggattg gaaaaccaat ttccgcacca tgagtgcgaa
     5821 atagcccagt cggaagcagc gacgggcaaa ccttttgtgc gttactggct gcacaacaat
     5881 atgctcacga tcaacgggca gaaaatgggc aagagcctcg ggaatttcgt taccatcaag
     5941 gaaatgcttc agaagatcag tccggaggcg ctgaggctgt tcatcctgat gagccattac
     6001 cgttcagtaa cggacttcag cgacgaagcg ctgaacagcg cggaaaaggg tatggcgcgg
     6061 ctttccgctg cagtacaggc gttatcgcag cgcctgccag gtgctgcaga aggtcccgcg
     6121 gatcgtaaga cggacgctga gctggcagag atacgccgca aattcgtcgt tgcgatggac
     6181 gatgacttca acactgcgga agcgatcgca cagctttttt ccgtggtcag gctgaccaac
     6241 agtgttcttt cggcgccgaa tccggcaacc agaggaacat tggaactact gaacacgcag
     6301 tttgacgaac tcggggaagg cgttctgggc ctgaggctga aggcacgggc tgagggaagt
     6361 gaggacctga cggatcagat aatgcaggtt ctgatccaga ttcgtgaaaa actgaggaaa
     6421 gaaaagcagt atgcgatggc ggacgagatt cgccagatgc tcgcagaagt gggcattgta
     6481 ttgaaggacg aaaaagggcg aaccacgtgg gctcgctcct gaagcgcgcg gttactggac
     6541 gtagaagaaa ttcgcggggt caaccgcccg accgtgatag aacacgctgt aatggagatg
     6601 cggacctgtt gaccggcccg tattcccaag ttccgcgatc gtctgtcctc ttgctacccg
     6661 ctgcccctct cgcacgagcg ctttcgccag gtgaccgtac acggtgctgt acccgtaccc
     6721 gtggtcgatc tgcacaacca atccggtgta tcctccccac gacacatcgg caacaacgcc
     6781 gtccgcggta gaaacgacgc tcgtgcctgt ccttccggcc agatcgatgc ctttgtggat
     6841 tcgtcgcctc cctgtatagg ggtcggcgcg atacccaaaa acggacgaaa catatacctg
     6901 tgccaggtca actggaggaa tactcgggag gtggtcacgg aactcaatgt tgttggcgag
     6961 cgcacgctct atttcccgga aactttcgcg ctggaacttc gcctgattca gaagcgactg
     7021 gatgtccgag tggacctcca tgccgaggcg ccctgcttct gtgacaacat c
//
LOCUS       DAOTNG010000157         7049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9291_length_7049_cov_5.434372, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000157 DAOTNG010000000
VERSION     DAOTNG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_7049_cov_5.434372"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="P5179_08615"
     CDS             complement(<1..929)
                     /locus_tag="P5179_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95316.1"
                     /translation="MEGTLITLQELGVSSEFPLPKRSDWGIGMVGFGGIARTSHAPAY
                     KKAGWKIVAVADPDEKARTLAKDEFGVEHVYADYHELIEDPAVEVIDLLTQPSVREEV
                     LLAAAAAGKHLITEKPLTQDPEEGVRMVAAAEKAGITFGVHQNYRWMTMNYLARQIVE
                     RGIIGDPFFVSIEIFGTQDRDLAGHHFYSVCDNFLTIQWNNHLADLVRYWTGRDAKRV
                     FARTGRSNGQNFVSDNLLSVIHDLGDGLTGHILHSELVRSDLTGVECRIDGAKGTLLF
                     DFHSTMRISSDFTGKGVHTLDVKEHEFRESFIG"
     gene            complement(929..1255)
                     /locus_tag="P5179_08620"
     CDS             complement(929..1255)
                     /locus_tag="P5179_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS95317.1"
                     /translation="MPIIQGPDAGVLTTEFAHWGLATFKTGMRNITRAHYHDCDEFVF
                     MIGGVMVMRSEGVEYTLRKDDVLVTRMGDEHEILEIVEDTTYFWLERELRGKKRTGHL
                     HRGEDD"
     gene            complement(1268..2383)
                     /locus_tag="P5179_08625"
     CDS             complement(1268..2383)
                     /locus_tag="P5179_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95318.1"
                     /translation="MRISLNGQRIFPLGAYEHPRTEDEWRQWTKAGINIVCCHNREEL
                     DAAHAHGVLGWVPVPVILADGDAGSTLAAKVDTLKDHPALAVWEAQDEAIWNASRKDD
                     NNVRMSFWKHPPEKLAEIEERFNAVVRGLERGAKVVREHDPGRPIWLNEAVNSDQETL
                     ARCVPYLDIVGFDWYPVRRNATVPMGLLGKQIQRFVRTAPLCGQWFVLQAFCWSQLKL
                     AEGPPLLPTEEQTRFMAWDAILHGATGIMWWGSAYADRPHPFFDGWMTVLREFEGLHP
                     FLFAGQMPHVWAETYYRQHDPILGVGVLARRAGNRTLVVLINQDQYAHETVLKGLDEA
                     VVMRLRRVGGGGGGLVKTREGFITALEGYDVRIYITD"
     gene            2975..4090
                     /locus_tag="P5179_08630"
     CDS             2975..4090
                     /locus_tag="P5179_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS95319.1"
                     /translation="MLTGKAALYALAAFVGFVAAGLLVAVGLVWMNSSGRPKPFVDSR
                     GNPLPGSISEKIFVDINGTKQGMFIKGKRRNAPVLLYLHGGMPDYFLTDKYPTGLDEL
                     FTVVWWEQRGSGISYDSGLDRSAMTVDTMMNDVITVADYLRQRFRQDRIYLMGHSGGT
                     FLGIRVAATHPELFRAYIGVSQISHQKASEKLAYDYMVRRFAAEGNADMVGKLRESPV
                     TLTGQIPDSYLKIRDKAMHELGIGTMRNMRNVFTGLFIPSLLFDEYSLGEKYRLWAGK
                     SHAGVSVIWNDIISTDLSEEITSLDIPVYFLHGRYDYTCSFALAEEFFRKIEAPVKGF
                     YVFEKSAHCPLFEEPLKTVGIIRDDVLRGRNDHADVK"
     gene            complement(4474..5307)
                     /locus_tag="P5179_08635"
     CDS             complement(4474..5307)
                     /locus_tag="P5179_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95320.1"
                     /translation="MASSQENTVRARAVRKRFPMGEGYLEALKGIDLEIKRGEYISIM
                     GPSGSGKTTLFNMIGGLDKPSDGTVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLI
                     PVMTALENVTLPMIFAGLSNDEAISKGRGLLDTVGLGERWQHKPTELSGGQQQRVAIA
                     RSFANDPSIILADEPTANLDLKTGHDIIELLRKLNKEMGVTIISNTHDPSMLNVSDRI
                     IWIRDGRVDRIEARSDIDINVGTIGGDVLGGVGSADSHGGAAGKHAMDRLTGEATNTG
                     E"
     gene            complement(5348..6034)
                     /locus_tag="P5179_08640"
     CDS             complement(5348..6034)
                     /locus_tag="P5179_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRS95321.1"
                     /translation="MDEQITHEEAIRRLRTDMKTSSVATPFATALKIAFQSLRIRFWR
                     SIITIAGIFFAIAFLVSVLNSAVVELAVNPDEGSQVSGTGLRFILTLLAAEPRQAWLV
                     TMSLIVCVIGIANAMLMSVTERYKEIGTMKCLGALDIFVIELFLLESGFQGLVGSFAG
                     ALIGAALVLVSALVKHGFAVMGILPWSQMVINILLATVIGLLLTLLGASFPAYRASQM
                     PPADAMRTEV"
     gene            complement(6063..6467)
                     /locus_tag="P5179_08645"
     CDS             complement(6063..6467)
                     /locus_tag="P5179_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRS95322.1"
                     /translation="MFGKLFKKREAPRLTKDDLLSSRPVLNQAISWSLNNQQEVQITI
                     PRREVWWATLLSKIIYTPKERVIALDEVGTAVWKMIERRLTVREMIAELSAQYNLNRR
                     EAEASLIEYLRRLAKKNFVGFEVPRQRRKTKR"
     gene            complement(6789..>7049)
                     /locus_tag="P5179_08650"
     CDS             complement(6789..>7049)
                     /locus_tag="P5179_08650"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HRS95323.1"
                     /translation="QEHSFQQSGLCDTERCYLELGHLVGANAVVVGSVGKVGRTYTVT
                     ARMIDVETGEILAQAAYDCPCEVDALLTNMQRIAQGLAGTGQ"
BASE COUNT         1359 a         2177 c         2068 g         1445 t
ORIGIN      
        1 ctcccgatga acgactcccg gaactcatgc tccttcacgt ccagtgtatg tacgcccttc
       61 cccgtgaaat cggaacttat ccgcatggtg gagtggaagt caaacagcag agtgcctttt
      121 gcaccgtcaa tgcggcattc tacgccggtc aaatcactgc ggacaagttc gctgtgcagg
      181 atgtgtcccg tgagtccgtc tccaagatca tgaatcactg acagcaggtt gtcgctcacg
      241 aagttctgcc cgttgcttcg cccggtgcgg gcaaacacgc gcttcgcatc gcgtccggtc
      301 caatatcgca ccaggtctgc cagatggttg ttccactgga tcgtaaggaa gttatcgcac
      361 actgagtaga agtggtgccc tgcgagatcg cgatcctgcg ttccaaatat ctcgatgctg
      421 acgaaaaacg gatcgccgat gataccgcgt tcgacgatct gtcgagcgag gtagttcatg
      481 gtcatccagc ggtagttctg gtgcacgccg aacgtgatgc ccgccttttc cgctgccgcc
      541 accatccgca ccccctcttc aggatcctgg gtgagcggtt tttcggtgat cagatgcttc
      601 cccgccgccg ccgccgcaag gagcacttcc tcccgcaccg agggctgggt gagcagatcg
      661 atgacttcga ctgccggatc ctcgatcagc tcgtggtaat cggcgtaaac atgctcgacg
      721 ccgaactcgt ccttcgccaa tgtccgtgcc ttctcatcag gatcggccac ggcgacgatc
      781 ttccagcccg cctttttgta cgccggcgca tgagaggtcc gtgcgatccc cccgaagccc
      841 accatcccga taccccagtc tgaccgcttc ggaagcggaa actcagagct gacgccgagt
      901 tcctgcagcg taatcagtgt gccttccatc agtcatcctc tccccggtgc aggtggccgg
      961 ttcgcttctt cccgcgaagc tccctctcga gccagaaata ggtggtgtct tcgacaattt
     1021 ccagtatttc gtgctcgtcg cccatgcggg taaccagcac gtcgtccttc cgcagggtgt
     1081 actcaacgcc ctccgagcgc atcaccatca cgcccccgat catgaacaca aactcgtcgc
     1141 aatcgtgata gtgcgcccgg gtgatgttcc gcatccccgt tttgaacgtg gcgaggcccc
     1201 agtgggcgaa ctcagtcgtg aggacacccg catcgggtcc ctgaatgatc ggcattgcgt
     1261 gctcctttca gtcggtgatg tagatgcgca cgtcgtagcc ttccagcgca gtgatgaacc
     1321 cctcgcgcgt tttcaccagg cctccgccgc ctccgccgac ccgtcgcagg cgcattacaa
     1381 cggcttcgtc cagccccttc aacaccgtct catgagcgta ctggtcctgg ttgatcagta
     1441 ccacgagggt gcggttgccc gcccttcgcg caagaacacc gacacccagg atcgggtcgt
     1501 gctgccggta gtacgtttcc gcccacacgt ggggcatctg cccggcaaag aggaaaggat
     1561 ggaggccttc gaactcacga agaaccgtca tccagccatc gaaaaacggg tgcggcctgt
     1621 ccgcgtaggc cgaaccccac cacatgatgc cggtggcccc gtggaggatc gcgtcccacg
     1681 ccatgaaccg ggtctgttcc tctgtcggca ggagtggggg gccttccgcc agcttgagtt
     1741 gcgaccagca gaacgcctgc agaacgaacc actgcccgca taatggggcg gttcggacga
     1801 atcgctggat ttgcttgccg agaaggccca tcggcaccgt ggcattgcgc cggacggggt
     1861 accagtcgaa gccgacgatg tcgagatacg gcacgcaccg cgccagcgtc tcctgatcgc
     1921 tgttcaccgc ctcgttgagc cagatcggcc tgccgggatc gtgttcccgt acgaccttgg
     1981 caccgcgttc caacccacga accaccgcgt tgaaacgctc ttcgatctct gccagtttct
     2041 ccggcgggtg cttccagaag ctcatccgca cgttgttgtc gtccttgcgg gacgcgttcc
     2101 agattgcttc gtcctgcgct tcccagaccg cgagggcggg gtggtccttc agcgtgtcca
     2161 ccttcgcggc aagcgtggaa ccagcgtcgc catcggccag aatcaccggc acgggcaccc
     2221 atcccagcac gccgtgcgcg tgtgccgcgt caagctcctc ccggttgtgg cagcagacga
     2281 tgttgatccc ggccttcgtc cattgccgcc attcgtcctc ggttcgcgga tgctcgtagg
     2341 cacccagcgg aaatatgcgt tggccgttca gagagatgcg catgagactc ctccacgaga
     2401 aggtgggtgc cgcgacgcgg gaccaccccg cgctgcgatg gtatgcgcga gtcgcggcag
     2461 aagaacagcg gccctttagg agaccggatg cgatgtcagc taatcccgca cgtcctgcca
     2521 tgcggcgagg aggtgttcgg aacgtgatgc tgacgtgatt gagaagatcg cttccgcggc
     2581 ggcgagggcg aacccacccg cgcgccggcg accggtgctg cgggttgcgc tccaaccgac
     2641 gggcattgcg gatagcgtca tgtggcgcac attctccggt ttcggtggac tgcctggacg
     2701 gggcggacag gacgccccct gcatctggct gtggcaaaga ggcgtcttcg tgtcaagagg
     2761 gggagcgaaa aaaagttcag cgggcggtgg aggggcacgc tgcaacgcct ccgcgtgccg
     2821 acaggagccc acggtttgcc tgtcgaaggt gctcgtgcta cgttactcct gatgatgcgg
     2881 ccgatttgga cagggccttg gcagtgcggg tgcacccgat tctcgaatgc ggttccgtgg
     2941 gagtactcca gaaatggacg ggagatatct cttcatgctg acgggaaaag cggcgctcta
     3001 tgcgctcgcc gcctttgtgg gttttgttgc agccgggttg ctggtggcgg tggggctggt
     3061 gtggatgaac agctccggca ggccaaaacc ctttgtggat tcccggggca atccgctccc
     3121 cgggagcatc tccgagaaaa tcttcgtaga catcaatggc acgaagcagg gaatgttcat
     3181 caagggaaaa cgcaggaacg cccccgtgtt gctgtacctg catggcggca tgccagacta
     3241 tttcctcacg gacaaatatc cgacgggact cgatgagctg ttcaccgtgg tctggtggga
     3301 acagcgaggg tcggggattt cctacgattc cggtctcgat cgttccgcga tgaccgtgga
     3361 caccatgatg aacgatgtca tcacggttgc agattacctc agacagagat tccgtcagga
     3421 caggatctat ttgatgggac attctggtgg gacatttctt ggaatcaggg tagcggcaac
     3481 acatccggag ttgttccggg cttacatcgg ggtctctcag atatctcacc agaaggcgtc
     3541 cgagaaactc gcgtacgatt acatggtgcg gcgattcgcg gcagaaggaa acgccgacat
     3601 ggtgggaaaa ctcagggagt cacccgtcac cctgaccggg caaatacccg actcctatct
     3661 caagatccgc gacaaagcca tgcatgaact cggcatcgga acaatgcgta acatgcgtaa
     3721 cgtgttcacc ggtctgttca ttccgtcgtt gctgttcgat gaatattcgc ttggggagaa
     3781 gtacaggctg tgggcgggca aatcgcacgc aggtgtaagc gttatctgga atgacatcat
     3841 ttccacggac ctgtctgaag agatcacgtc actcgatatc cctgtctact tcctccacgg
     3901 gagatacgat tacacctgct cgtttgcgct ggcagaggag ttcttcagaa aaatcgaggc
     3961 accggtcaag ggattttacg tgttcgagaa gtcggcgcat tgtccgctat ttgaggaacc
     4021 actgaaaacc gtgggaatta tcagagatga tgtcctacgg gggaggaacg atcatgcgga
     4081 cgtgaaatag aactcatgcg gcacccgccc gcgcgaaatc gggaacctcc cccagggtca
     4141 cgacgaagcg tgaccctcca ccccgtcacg ccggatcccc gcctgcgcgc ctcacgttaa
     4201 ggaagacaca tgtacggttt ttcgcggcgg aacagggtcg gcatgcgacc ggaggaagag
     4261 cgcggggtca ttgtggttgc tcccgtacac acgcagagcc gctcagagac atgtctccgc
     4321 ggccctgtgg tctgctggaa agcgttgtgc gtgcccgccg cgggcgaggc tcagtgccgc
     4381 ttagtgctac tctcccgtat tcgttgcttc tccggttaaa cggtccatgg cgtgcttgcc
     4441 cgccacgggc aagaggctca gtgccgcttg ccgctactct cctgtattcg tggcttctcc
     4501 agtcagacga tccatggcgt gcttgcccgc agcgccgccg tggctgtccg ccgagccgac
     4561 gccgccgagc acgtcgccac cgatggttcc gacattgatg tcgatatccg agcgggcttc
     4621 tatgcggtcc acgcgcccgt cgcgaatcca gatgatgcgg tcggagacgt tcaacatgct
     4681 ggggtcgtgc gtgttggaga tgatggtgac gcccatctcc ttgttgagct tgcggaggag
     4741 ttcgatgatg tcgtgcccgg ttttcaggtc gaggtttgcc gtcggttcgt ccgccagaat
     4801 gatggaggga tcattcgcga aggaccgcgc aatcgcaacg cgctgctgct gcccgccgga
     4861 aagctccgtc ggcttgtgct gccaccgttc cccgaggccc acggtatcca gcaggccgcg
     4921 ccccttcgag atcgcctcat cgtttgaaag cccggcgaaa atcatcggca gggtgacgtt
     4981 ttccagcgcc gtcatcacgg ggatgaggtt gaatgtctgg aaaatgtagc cgatcttccg
     5041 gcaacggagc catgcgagct cgtacgcgtc caactgggct atatccacct cgtcgatgta
     5101 caccgttcca tcagagggtt tgtccagacc gccgatcatg ttgaaaagcg tggttttgcc
     5161 ggagccggac ggacccatga tgctgatgta ctcgccgcgc ttgatttcca gatcgatgcc
     5221 cttcagcgcc tccagatagc cctcacccat cgggaaccgt ttccggacgg cgcgcgcgcg
     5281 aacggtgttt tcctgtgaac ttgccatcga gctaacccct cattcgagat gaagcagtga
     5341 atatctgtca gacttccgtg cgcattgcgt ctgccggtgg catctgggat gcgcggtatg
     5401 cgggaaaact ggcacccagc agcgtcaaca aaagcccgat gaccgttgcc agcaggatgt
     5461 tgatcaccat ctgcgaccag ggcagaattc ccataacggc gaacccgtgt ttgaccagcg
     5521 cactcaccag caccagcgcc gcaccgatca gcgcgccggc aaacgaccct accagcccct
     5581 ggaacccgga ttcgagcaaa aacagctcaa tcacgaatat gtccagcgct ccgaggcact
     5641 tcatcgttcc gatttccttg tagcgctcgg ttaccgacat cagcatcgcg ttggcaattc
     5701 cgatgacgca tacgatcagc gacatggtaa ccagccatgc ctgacgtggc tccgcggcga
     5761 gcagggtcag gatgaacctt aggccggttc cggatacctg cgagccctcg tccggattca
     5821 ccgccagttc gacaaccgcg gaattcagga cggagaccag gaaggcgatg gcgaaaaaga
     5881 tgcccgcgat agtaatgatc gaccgccaga accttatgcg gaggctctga aacgcgattt
     5941 tcagcgccgt ggcgaacggc gttgccacgc tggaagtctt catgtcggtg cgcagacgcc
     6001 ggattgcttc ttcgtgggtg atctgttcat ccatgggtgc ctcgtttcaa gccttccgcc
     6061 cgttatcgct tcgttttccg ccgctgccgc ggcacttcaa aaccgacgaa gttcttcttc
     6121 gccagcctcc ggagatactc gatcagcgac gcttccgcct cccggcggtt gaggttgtac
     6181 tgcgcgctga gttcggcaat catctcccgc accgtgaggc ggcgctcgat catcttccac
     6241 acggccgtgc ccacctcatc cagcgcgata acccgctcct tcggggtgta gatgatcttg
     6301 gaaagcagcg ttgcccacca cacttcgcga cgcgggatcg tgatctgcac ttcctgctga
     6361 ttgttcagcg accagcttat tgcctgattc aacaccggcc gcgagctgag caggtcatcc
     6421 ttagtgagcc ggggcgcttc ccgctttttg aacagctttc cgaacatact ccccgggaga
     6481 ctttcgtgct gatggagacg gtggattacg agaaggtaag aataacggga aaaaggttgc
     6541 gcgcgcaagg cggtgtctgt gcgaacgaaa acgagccgcc ccacccgtcg gcggggaaac
     6601 gcgtgggaac acgcacgctc caccgttcca gcagcggaag gttgtcccgc gattcaccga
     6661 tgtggacatc cagccgttca tgctgctgaa acgacggtcc actgccggat cccgccgtca
     6721 ttcccgcgtg ctcggcggcg cacgaagcga aggcgggaat ccagcgccgc gatgcatgtg
     6781 gcaatgtgtt attgcccggt tccggcgagg ccctgggcga tacgctgcat attcgtgagc
     6841 agcgcatcca cctcgcacgg gcaatcatac gccgcctgcg ccaggatttc gcccgtctcg
     6901 acatctatca tccgcgccgt gacggtgtac gtgcgcccca ccttgcccac tgagccaacc
     6961 accacggcgt ttgccccgac aaggtggccc aattccagat agcaccgctc cgtgtcgcac
     7021 agccccgatt gctgaaagct gtgctcctg
//
LOCUS       DAOTNG010000158         7031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9315_length_7031_cov_4.682339, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000158 DAOTNG010000000
VERSION     DAOTNG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_7031_cov_4.682339"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..368
                     /gene="nuoK"
                     /locus_tag="P5179_08655"
     CDS             66..368
                     /gene="nuoK"
                     /locus_tag="P5179_08655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRS95324.1"
                     /translation="MIQTIHVLWLSTILFVIGTAGVLIRRNAIVMLMSVELMLNAVNL
                     ALIGFARQFRDVTGQVYVIFIMTVAAAEVAIGLALFVAVFRHKRTLDTQKIDLLRW"
     gene            444..2333
                     /gene="nuoL"
                     /locus_tag="P5179_08660"
     CDS             444..2333
                     /gene="nuoL"
                     /locus_tag="P5179_08660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRS95325.1"
                     /translation="MTTAIALIPFWPLLGFLINILFGKRLRSASAYLAIGMLALSFLT
                     SVAVFARFVGDPASVLVPLFSWMSVGALNVEVNFLVDQLTVVMLLVVTGVGTLIHVYS
                     VGYMHGDERYSRFFAYMNLFAAMMLVLVLADNYVVMFVGWEGVGLCSYLLIGFWFERP
                     FGSGTTNDAARKAFIVNRVGDFGFLLGVFLIWTAVGGVSFQQTNSAVESGYVTPGVAT
                     IATLLLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARSHFLFD
                     IAPFSQNVVLWVGALTALFAASMGVQENDIKKVLAYSTISQLGYMFMGVGAGAYAAGV
                     FHLMTHAFFKALLFLGAGAVIHALHGEQDITRMGGLRNRMPLVHGTFLIGALALAGFP
                     LTAGFFSKDSVLAGVYAHGDFAVWCIGVLTAGVTAFYTFRLYFLVFSGSSRDEKGRDL
                     HAPASSMTSILLILAILSLVGGYVELPFHILAAFSAFLSPVVGMSSHEGIPELALMTV
                     VFLFVLGGIGVSWAWYGRREQDATALAAEPANPFLRTLRRKWYVDEIYEVLLIRPIRW
                     TSRTVLWRVIDEWIIHGFLVQFCGGYLWKAIGFVVSFSHTGRVGSYAFGIVVGVLLVI
                     SLIAR"
     gene            2364..3962
                     /locus_tag="P5179_08665"
     CDS             2364..3962
                     /locus_tag="P5179_08665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS95326.1"
                     /translation="MALSTLILLPVIAALILGFLPESRKELVRNVALVFALMVFAYSV
                     GMYFQFDPSTPRLQFVENRSWIDAIGARYHLGVDGVSIFLVLLTTLLTPVAILASYAA
                     IGERVRMFNAMLLLLEAGTLGVFLAQDLVLFYVFWEVMLVPMYFIIGIWGSSERLKAT
                     FVFVLYTMAGSVLMLVAIVALAIVNAQNVAAALSAPGAYMGYVSFDISSIRTELLTGT
                     QQIWLFAAFFLAFAIKVPLFPLHTWLPLAHTEAPTAGSVILAGVLLKTGGYAIIRFCL
                     GLFPEAAVVYAPYINTLAVVGIVYGALVSLVQEDLKRLVAYSSVSHMGFILLGVFSFT
                     QEGMTGGIVQMVNHGISTGGLFLCVGMLYERSHTRKLEELGGTATAMPLFAGLFLVIT
                     LSSAALPGLNGFVGEFLALAGAFSNKATRWFSVVGATGMILAAAYLLWMFQQAMYTTK
                     PGVWVKRTWGDLTVREVLVLLPIVLSAVGIGVYPAPLLKPIAASSQLVLQRMNLRPNG
                     PIHAIPSGVDDLATTSPDLPEASR"
     gene            3959..5404
                     /locus_tag="P5179_08670"
     CDS             3959..5404
                     /locus_tag="P5179_08670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRS95327.1"
                     /translation="MISASDIAVLLPEIVLVAGAIILLMLDAFSPRITRRFGALLASV
                     VLFAALLAVQQGEPSKFPAFGALVSDAFTDMVAVSVLLFGFVAVISAWSYLEERGLAS
                     GEFMAAMLATSAGMILISRSSHLMVIFVALEILSIGLYMLIGYDRTDVLSAEAGLKYF
                     ILGAFASALFLFGAVLLYGVTGTMQLTLEQLPFHTRAGQVGIALCIAGLGFKISAAPF
                     HLWTPDVYQGAPTPVTAFLSSASKIAAFAALLRVVLPSFSPFAEGWHAVWVALAALTM
                     FVGNVAALVQENVKRILAYSSIAHVGFMLMALAGGATVGPVGIEGLLFYLLTYAVSAA
                     GAFAAVAVIPGSPDLHTIRGLAKSHPSMAALFALFLLSLAGVPPLVGFLGKLYAFRSA
                     VDAGMYKLAAFAALNTAMGMYYYLRIIVRMYMEQRAEPAAFVVPQVAYPVLVLAALAV
                     VLLGLFPDPVLVLVRQSAEAVVPLARAPVAF"
     gene            5536..6747
                     /gene="tyrS"
                     /locus_tag="P5179_08675"
     CDS             5536..6747
                     /gene="tyrS"
                     /locus_tag="P5179_08675"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRS95328.1"
                     /translation="MLPFNEQMDLIRRGVAEIVSEDELAQRISEAVRSGKPLRIKYGI
                     DPSGKDVHIGHMVPVSKMRDFQDAGHHGVLIIGDYTAQIGDPTGRNESRPSLSKEQVL
                     ANAATYVDQLMKVLDPEKTEVRYQSEWFENFSLIQMIQLGQQFTMAQMLSHETFAKRL
                     EEGLPLAMHELMYPMLMAYDSVAVQSDVELGGMEQRFNILQGRDLQRAMGQRPQIAVL
                     SPMLRGLDGGPKMGKSLNNYIGVNDSPADMFGKIMSIRDDLLIDYLTLAAGVSQDAVR
                     AAEARLRRGTNPMEIKRELGRAVVQRYHGAQAAIAAEEEFLGVFSRRDQLPEDMPEFT
                     LDENPVRIVDILVKTESAPSMSEARRLIQQGGVTLDGYRVTDANAEIFVKDGAILKVG
                     KRRYARLRLKE"
     gene            complement(6839..>7031)
                     /locus_tag="P5179_08680"
     CDS             complement(6839..>7031)
                     /locus_tag="P5179_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019064102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS95329.1"
                     /translation="GDVRPRENHPKEVPPGSIMDVKTCLGRSGFPEELANVALFLASD
                     AASYVTGAAIMADGGWTSI"
BASE COUNT         1292 a         2029 c         2021 g         1689 t
ORIGIN      
        1 gaccgaccgg caggatcgaa cagcacagga acggccggag acgctccctg cggagaaacc
       61 gccccatgat ccagaccata cacgttctct ggctcagcac gattcttttc gtcattggga
      121 cggccggtgt tctgatccgc cgtaacgcca tagtgatgct gatgtccgtg gaactcatgc
      181 tgaacgccgt gaatctggcg ctcatcggtt ttgcccggca attccgtgac gttacgggac
      241 aggtgtacgt gatcttcata atgacagtgg cggccgcaga agtcgccatc ggccttgcac
      301 tttttgtcgc cgtgtttcga cacaagcgca cgctcgacac gcagaagata gacctcttac
      361 ggtggtgaat ccatcaccgt ttttgttgcc gaacaacgat ctgttccctt atagacgcgc
      421 agggcacacg tgagaacacg aatatgacca ccgcaatagc actgataccc ttctggcctc
      481 ttctgggctt cctgatcaac attcttttcg ggaagcgcct gcggagcgca agcgcgtatc
      541 ttgccatcgg catgctggct ctgtcgtttc tcaccagcgt tgcggtattt gcacggtttg
      601 tcggcgaccc cgcctcggtc ctcgtcccac ttttttcctg gatgtccgtc ggcgccctga
      661 acgttgaggt caacttcctg gttgatcagc tcacggtagt gatgctgctt gtggtaaccg
      721 gcgtcggaac gctcatccac gtgtacagcg tggggtacat gcacggcgat gaacgttaca
      781 gccgtttctt cgcctacatg aacctgttcg cggcgatgat gctggtgctc gtcctcgcgg
      841 acaactacgt cgtcatgttc gtcgggtggg aaggcgtggg gctgtgctca tacctgctca
      901 tcggtttctg gttcgagcgt cctttcggga gcggcaccac aaatgatgct gcccgcaagg
      961 cgttcatcgt caatcgcgtc ggggatttcg gatttctcct cggggtgttt ctcatctgga
     1021 cggccgtagg cggagtgtct ttccagcaga caaattcggc cgtggaatcg ggatatgtca
     1081 ctcccggcgt tgccaccatt gccacgttat tgcttttcat cggtgcaacc ggaaaatcag
     1141 cccagatccc gctgtacgtc tggttgcccg atgcaatggc cggaccaact cctgtcagcg
     1201 cactgatcca cgccgcgaca atggtgactg ccggcgtcta catggtagcc cgcagtcatt
     1261 tcctgttcga tatcgctccg ttttcccaga acgtggtgct ctgggtgggc gctcttacgg
     1321 cactgtttgc cgcaagtatg ggcgtgcagg agaacgatat caaaaaggtc ctcgcatact
     1381 cgacgatcag ccagctcggt tacatgttca tgggcgtcgg cgcgggcgca tacgccgccg
     1441 gggtgttcca cctcatgacg cacgcgttct tcaaagctct cctcttcctt ggtgcgggcg
     1501 cggtcatcca cgcgctacac ggggaacaag acatcaccag gatgggcggg ttgaggaacc
     1561 gaatgcccct tgtgcacgga accttcctta ttggagccct cgcgcttgcg ggtttccccc
     1621 ttaccgccgg tttcttttcc aaggactcgg ttctcgcggg agtgtacgct cacggcgatt
     1681 ttgccgtgtg gtgcatcggt gttcttacag cgggcgtgac ggccttctac acattcaggc
     1741 tgtacttcct tgtgttttcc gggtcttccc gcgatgaaaa ggggcgtgat ctgcatgctc
     1801 cggcatcatc aatgacttcg attctgctga ttctcgcgat tctcagtctt gtgggcggct
     1861 acgtggagtt gcccttccac attttggccg cgttctcggc gtttctgagc cctgttgtcg
     1921 gcatgagcag ccacgaaggc attccagaac tggccctcat gacggtagtc ttcctgttcg
     1981 tccttggcgg aataggcgtg tcgtgggcct ggtatggcag aagagagcag gacgccacgg
     2041 cgcttgcagc ggagcccgca aatcctttcc tgcgcacgct gcgtcgcaaa tggtacgtag
     2101 atgagatcta cgaggtcctg ttgatccgcc cgatacggtg gacttccaga acggtgttgt
     2161 ggcgcgtgat tgacgaatgg ataatccatg ggttccttgt ccagttctgc ggagggtatc
     2221 tgtggaaggc gatagggttt gtggtgagtt tctctcacac gggccgcgtc ggcagttacg
     2281 cctttggcat cgtggtcggt gtgctgttgg tcatttctct tatcgcccgt taggcatgta
     2341 gctcgcggat acgagatact cgcatggctc tgagcactct catccttctt ccggtgatcg
     2401 ccgcgctcat tctggggttc ctgcccgaat cccgcaagga gctggtgcgg aacgttgcgc
     2461 tggtattcgc gctcatggtg ttcgcgtatt cggtggggat gtatttccag tttgatccct
     2521 ccacccctcg gctccagttc gtagaaaaca ggtcttggat cgacgcaatc ggtgcacggt
     2581 accaccttgg ggttgacggg gtaagcatct tcctcgtcct gctgacaaca cttctcaccc
     2641 cggttgcaat ccttgcctcg tacgcggcaa ttggcgaacg cgtgcgcatg ttcaacgcga
     2701 tgctcctcct gcttgaagcc ggcactctgg gggtttttct cgctcaggat cttgtgctct
     2761 tctacgtgtt ctgggaggtc atgctggtgc cgatgtattt catcatcggc atctggggaa
     2821 gctcggaaag gctgaaagcg acgtttgttt tcgtgttgta cacgatggca ggctccgtac
     2881 tgatgcttgt ggcgatcgtc gcactggcaa ttgtgaacgc acagaacgtg gctgcggcgc
     2941 tttcggcgcc cggcgcgtac atggggtatg tatcgttcga catctcctcg atcagaaccg
     3001 agcttctgac aggcacgcag cagatctggc ttttcgccgc atttttcctt gccttcgcga
     3061 tcaaggtgcc gctgttcccg cttcatacgt ggttgccact tgcgcacacc gaggcaccca
     3121 ccgccggatc ggtaatcctc gcgggagtgc tgctgaaaac aggtgggtat gcaataatcc
     3181 gtttctgcct cgggctgttt ccggaagccg cggtcgtcta tgcaccctac atcaacactc
     3241 tggcagtggt aggtatcgtg tatggagccc tcgtttcgct cgtccaggag gacctgaaaa
     3301 gactcgtcgc ctattcctcg gtgagccaca tggggttcat tcttctgggg gtgttctcct
     3361 tcacccagga aggaatgacg ggtggaatcg tgcagatggt aaaccacggc atttccaccg
     3421 gcggtttgtt cctctgcgtc ggcatgctgt atgaacggtc acacacccgg aaactcgagg
     3481 aactgggagg gacagcaaca gcgatgccgc tgttcgccgg tctttttctt gttatcacac
     3541 tttccagcgc ggcacttccg ggattgaacg ggtttgtcgg cgaattcctg gctctcgccg
     3601 gcgcattttc gaacaaagcc acgcgctggt tttctgtggt cggagctacc gggatgatcc
     3661 tggcggcggc gtacctcctc tggatgttcc agcaggccat gtataccacg aaacccggtg
     3721 tatgggtgaa acgcacgtgg ggagacctca cggttcgtga agtgctcgtg cttttaccta
     3781 tcgttctttc cgcagtggga atcggggtgt atccggcacc tcttctgaaa ccgatcgctg
     3841 catccagcca actcgttctt cagaggatga atcttcgtcc gaacggccct atccacgcca
     3901 tcccgtccgg cgtggacgac cttgcgacaa cctcccctga cctaccggag gcgagccgat
     3961 gatctccgcc tccgacatcg ccgttttgct gcccgagatc gttctggttg cgggagccat
     4021 catcctcctg atgctcgacg cgttctctcc gcggatcacg cgtagattcg gcgcattgct
     4081 ggcatccgtc gtgcttttcg cggcgctgct tgccgttcag cagggtgaac caagcaagtt
     4141 tcccgcattc ggagcgctcg tcagcgacgc gttcacggac atggtggccg tttccgtctt
     4201 gttgttcggc ttcgtcgcgg tgatctcggc gtggagttat ctcgaggagc gaggactggc
     4261 gtcaggcgag ttcatggcgg caatgctggc cacatccgcc ggaatgatcc ttatctcccg
     4321 ctcaagccat cttatggtga tattcgttgc gctggaaatc ctgtccatcg gcctgtacat
     4381 gctgattggc tatgaccgga cagacgtctt gagcgctgaa gccggactca aatacttcat
     4441 cctcggcgcg tttgcgtccg ctcttttcct tttcggcgca gtcctgctct acggcgttac
     4501 cggaacaatg cagcttacac tggaacaact cccatttcac acgagggcgg gtcaggtcgg
     4561 aattgccctg tgtattgccg gactggggtt caaaatctcg gccgccccgt ttcacctctg
     4621 gactccggac gtgtaccagg gagcacccac gccggtaacg gcgtttctat cttcggcttc
     4681 gaaaatcgcc gcttttgcag ctctgcttcg agtagttctg ccctcctttt cgccatttgc
     4741 ggaaggatgg cacgccgtgt gggtagcgct cgcggcgctt accatgtttg tgggcaacgt
     4801 cgcggcgctc gttcaggaga acgtgaaacg catcctcgca tattccagta tcgctcacgt
     4861 cgggtttatg ctcatggcgc ttgcaggggg cgcaaccgtt ggccctgtgg ggattgaagg
     4921 gcttctgttt tatctcctta catacgccgt cagcgctgca ggagcattcg ctgccgtggc
     4981 cgtaatcccg ggttcgccgg acctccacac tattcgcggt ctggcaaaat cccacccttc
     5041 gatggcagcg ctcttcgcgc tgttccttct ctcccttgcc ggggtaccac cacttgttgg
     5101 tttcctcggc aaactgtacg ccttccgttc tgctgtggat gccggaatgt acaagcttgc
     5161 ggcgttcgca gccttgaaca ccgccatggg aatgtattat tatcttcgga tcatcgtgcg
     5221 gatgtacatg gagcaacgcg cagaaccggc ggcgtttgtt gtcccccagg tggcataccc
     5281 ggtactcgta ttggcagctc ttgcagttgt gcttctcggg ctgtttccgg acccggttct
     5341 ggtgcttgtc aggcaatccg cggaagcagt ggttccgctt gccagggcgc ccgttgcgtt
     5401 ctgaaagccc gcgcggcgtt cgaccgattg gtgaatcacg aagttgcgcg ttctcaaacc
     5461 ttttctctcg ttaccggggc aaacccgtag tgagtagtga tcgcgcgcaa aacccccatg
     5521 tgaggaaacg gcaaagtgct cccttttaac gaacagatgg acctaatccg ccgcggtgtt
     5581 gccgaaatcg tgtctgaaga tgaactcgcg caacgaattt cggaagccgt gcgaagtgga
     5641 aaacccttac ggatcaaata cggtattgac ccttccggca aggacgtaca tatcggtcac
     5701 atggtccccg taagcaaaat gcgcgacttc caggatgcgg gtcaccacgg cgtgctcatc
     5761 atcggtgact acactgccca gatcggagat ccaacaggac ggaatgaatc gcggccgtct
     5821 ctgtcgaaag aacaggtgct cgcgaacgcg gccacctacg ttgaccagtt gatgaaggtg
     5881 ctcgaccctg aaaagaccga ggtgcgttac cagtcagaat ggttcgagaa cttctctctg
     5941 atccagatga tacaactggg gcagcagttc accatggctc agatgttgag ccacgagacg
     6001 ttcgcgaaac ggctcgaaga agggcttcct ctcgcaatgc acgagctgat gtatccgatg
     6061 ctgatggcat acgactcggt cgccgttcag tcagatgtgg agctcggagg aatggaacag
     6121 cgcttcaata tccttcaggg cagggatttg cagcgcgcga tgggtcagcg cccgcagatt
     6181 gccgttcttt cccccatgtt acgtgggttg gatggcggtc cgaagatggg aaaaagcctg
     6241 aacaattata tcggggtgaa cgattcgcca gcagatatgt tcgggaaaat catgtccata
     6301 cgcgatgatc tgctcatcga ttaccttact ctggcagcgg gagtgtcgca ggacgcagtc
     6361 cgcgcggcgg aggcgagact tcggcgcggc acgaacccga tggaaatcaa acgggaactc
     6421 gggcgcgcgg ttgtgcaacg gtaccacggc gcgcaggcag ctattgcggc tgaggaagag
     6481 ttcctcggcg ttttctcgcg tcgcgatcaa ctgccggaag acatgcccga gttcactctg
     6541 gacgagaacc cggtgcgcat agtcgacatc ctcgtgaaaa cggaatccgc accctccatg
     6601 tcagaggcgc gccgtctcat tcaacagggt ggcgtaacgc tcgacgggta ccgagtgact
     6661 gatgccaacg cggagatatt tgtcaaagac ggcgccatcc tcaaagtagg caaacgccga
     6721 tacgcccggt tgcggctcaa agagtagcag cagccactcg aaaagtgaaa gagggcgcgg
     6781 aaaactttca ttccccgccc tctttcactc tctcccggcg caactcacac ctggcgaatc
     6841 agatggacgt ccaccccccg tcagccatga tggcggcccc cgtcacatac gaagcggcgt
     6901 cagaagcgag aaagagcgcc acgttggcca gttcctcagg aaatccgctg cgccccagac
     6961 aggtcttgac gtccattata ctgcccggtg ggacctcctt ggggtgattc tcacgcggcc
     7021 tcacgtcacc g
//
LOCUS       DAOTNG010000159         7028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9321_length_7028_cov_5.181127, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000159 DAOTNG010000000
VERSION     DAOTNG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_7028_cov_5.181127"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            674..1984
                     /locus_tag="P5179_08685"
     CDS             674..1984
                     /locus_tag="P5179_08685"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS95330.1"
                     /translation="MDPRSRRSILGWALYDFAESAFATSILVAILPVYYLSIAPQGGI
                     AVAFGPIRFTTAASSLWAYTISFSTMLIALPSPVLGALADSGGRRKLFLGVFGYTGAL
                     FSALLVFVGTGDYLLASALFCVANIGSVGSSTFLNALLLDVAPPGKMDSVSGKGFAAG
                     YLGGGTVFVINLLMIAHPEWFGIPSQEWGIRLSFLVVGLWWALFSIPTMLWVRERPRE
                     EKTHGSRIVREAFRTTWMTLRRITRFRDLSLYLLAFLVFNDGIQTVIVMAVPYAKETL
                     RLDTTTLMGVLVLIQAIGIPGSLLFGWVAERLGSKRALLISLTIWSGVVIMAWRMTSA
                     VEFWVLGACVGLVLGGSQAISRSLYGALIPQARAAEFYGFLAVSSRFSSFLGPLVFGL
                     ARDITGSMRAGILALIVFFIVGSGLLLLVNMQRGIERARAEEAG"
     gene            complement(2147..3358)
                     /locus_tag="P5179_08690"
     CDS             complement(2147..3358)
                     /locus_tag="P5179_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95331.1"
                     /translation="MTAPANTDRLVWWKEARFGMFIHFGVYSLVSHGEWIYYQEHIPQ
                     EEYARLADRFVPEHYNPREWVRIAQDAGMRYMVMVTRHHDGFCMYDSKVSDFTSTKRA
                     AKRDFIAEFADACHEAGMRMGLYYSLVDWRFPGALFNIPRQADEVYAPMVEQAHAQVR
                     EILTNYGKVDILWYDMAVPAGVWRPEELNAMARELQPGILINNRAGTPEDFGTPENAI
                     VPEKRAWEACYTMNETWGYAEGDRNWKSANQLIYLLATCASGGGNLLLNVGPEPDGRF
                     PREAVDRLRQVGAWMRTNGAAIYGAQPSPVTAPALGWGTYAGGKVYLIILRWPGSTVP
                     FAWCANRVLKAKLLITGEEARIEQKGDRVWLHGFPEYPPDNLPSVVELTLEGEPQAAV
                     PSLAFGNKLEV"
     gene            complement(3442..4515)
                     /locus_tag="P5179_08695"
     CDS             complement(3442..4515)
                     /locus_tag="P5179_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95332.1"
                     /translation="MTGRERLRAILRKQPKDRLAWTTLVDNNTLALLPEHLRGNGGID
                     FYKHLGCDIFLLNGWNTPHDLRAPVHQWPESVAVSYEQEGARVTTRWRTPHGELTGIT
                     QNGHPIKYPVDSIDAVRIYTAMWEGVTFHPYHDHPALAALDELIGEYGVVTRFWGPST
                     IPRLLEQDMGTQHFYYLLADHPGDMRALVRTMHERELEAFKLLAEGPWESVTLVENTS
                     TFYISPAVYREFNMPHQRDFVETVKATGKTALLHMCGHVHRILDLIRQTGCDGIHALT
                     PPPTGDTPWEAALDVLGEDTIIFGVLDPTIFVAGDVEEIAPSLDRLITPRLRKANFVL
                     CPFADGIPVPMERFLAVKEWAGR"
     gene            complement(4538..>7028)
                     /locus_tag="P5179_08700"
     CDS             complement(4538..>7028)
                     /locus_tag="P5179_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95333.1"
                     /translation="QSGRLFRFDEDGRVVWDFDTQAFCYAPGAIVDVDGDGALEYVLC
                     TELGKILVYNQAGEIVFTHQCDSRRAHRTTPAFGKIADGPRGLVFGITGSDTGRLYCF
                     TVPASADTHVEWHAFRGSPRSTGSWFGLQKVEAVRMEPENLVWDAVLTQDEITFRVTN
                     PAPGKEPLEAEATVLAPDGVRQGATGIIAGEKGVLKMPVAVSAPGKYRFEWCARKADG
                     EVLARGSRALTLSPYRNDQALARKAVVALRDAVAGVPTDAGFPAVLREECAAIEQEAG
                     NLAVLQLAGAASAFSPEVVARTGKLNARAKRALALAGIAPTVLSDASGCPVLPFEGRM
                     WENLNVDAELPGRLFLPLAIRRRCVTGEHEPVSIKLLNVSPDTVMVRTTVGEVPGGLV
                     ITPHKVTQVPTNPDSGKVVWDPIIPLGEVESVAIPRLETREFWFDVDAAKATAGEHTV
                     KVTFSAGGKETVVEIALRVLPFEMGGYDAMRLCMWASYNQHTVPDLLAHGATVFLTGL
                     PPAKAVEQGGQTRLEVDFTTMDQFLAPLEGHDVFLLLTGLPQVGVGEDDPSYVARLAD
                     YFEQVMSHLAAKGIPEEHVALYTWDEVGGNGWDAVRRYVAFGKLVLKARPTIKFYVNG
                     GGDLPMFEELVEIAAIWSPAFFMLDDDTPVMRYLWNTKKTMWTYNCAYMYARPMGWNT
                     KAINIVGEFRMQAVFAVRYGATGIGYWCYNHGESMWNPVHAEYPIVYQTSPDGPVTSS
                     RRWEAVRESVEDSRIVVALREKLADASVSDAAKAKIRHLVEVTLPGMADKSLTEMHLG
                     TAPYVLDDTNNDAMVAAFREELLDCVELVSG"
BASE COUNT         1145 a         2271 c         2210 g         1402 t
ORIGIN      
        1 cggcaacgga gcgcaccagt ggacgagccc gatctggatt gacctcgagt gaggtgccga
       61 gggcggtctc tggttctctc agtggtggaa cacgcccggc cggtggtgcg tgccgtttcc
      121 gggcaatgcg tgcatgaatc tggaaccgtt tgtggcggag cagctggcga tcggtgaatc
      181 tgcatccatc aatggccgga tcggcatgca tgaggggtac cgggcgtcgc tggcggggtt
      241 gttcgggggc ttgggggcat tcgtggcgct ctctgacaga gcacaccacc tttcagaagt
      301 gcgtacaccg cccggtcctg cgaaccgggc tgggctgttt cgcgccgttg gcgctgggcg
      361 cccgctgttg cgggcgaaag gaagggctga aaggccgcaa taatccagcc cggctcatcg
      421 ggccgggcgc ccgtgccgag ccgggagcgg cggcttgttg ggaggccgcc gtggacagtg
      481 cggagggcag gcaggaatgc ctgccccacc gatatttgtt gcgaccggcc gcagggtggg
      541 acaggcattt tgcctgtcca gtgcctcctc cctcctccag tggttgcctt cgtccgttct
      601 cccgtgtttc gtatcctttt cgcaggactt cggctgttcc gcttcttctg cccgcacatg
      661 aggaggcatt cgcgtggatc cccgttcgcg tcgctccatc ctcgggtggg cgctgtatga
      721 tttcgcggaa tcggcgttcg ccacctccat cctcgttgcc atcctgcccg tctactacct
      781 ctccatcgct ccgcagggcg gcatcgcggt ggcgttcggc ccgatcaggt tcaccaccgc
      841 ggcatccagc ctctgggcgt atacgatcag cttctccacc atgctgatcg ccctcccttc
      901 gccggtgctg ggggcgctgg cggattcggg aggccgcagg aagctcttcc tcggcgtttt
      961 cgggtacacc ggggcgctgt tcagcgcgct gctggtgttc gtcggcacgg gggactacct
     1021 cctcgcgtcg gcgctcttct gcgtcgcgaa cattggctcc gtaggcagca gcacctttct
     1081 gaacgcgctg ctgctggacg tggccccacc cggcaaaatg gattcggtct ccggcaaagg
     1141 ctttgcggca ggctatcttg gcggcgggac ggtgttcgtc atcaacctgc ttatgattgc
     1201 gcacccggag tggttcggca ttccctcgca ggagtggggc atccgcctga gtttccttgt
     1261 tgtcgggctc tggtgggcgc tgttcagcat tcccacgatg ctctgggtgc gggagagacc
     1321 gcgggaagaa aagacgcacg gctcccgcat cgtccgggag gcattccgca cgacgtggat
     1381 gacgctccgg cgcatcacgc ggttccgcga cctttcgctg tatctccttg ccttccttgt
     1441 gttcaatgac ggcatccaga cggtgatcgt gatggcggtg ccgtatgcca aggaaacgct
     1501 caggctcgat acgaccaccc tgatgggcgt gctggtgctg attcaggcca tcggtatacc
     1561 ggggtcgctg ctgttcgggt gggtggccga gcggctgggt tccaagcggg cgctcctcat
     1621 cagcctcacc atctggtcgg gcgtcgtcat catggcgtgg cgcatgacca gtgccgtgga
     1681 gttctgggtg ctgggcgcct gtgtcgggct ggtgctgggc ggcagtcagg caatcagccg
     1741 ttcgctgtac ggcgcgctga ttccccaggc gcgcgcggcg gagttctacg gattcctcgc
     1801 ggtcagcagc cgattttcgt cattcctcgg tccgctcgtc ttcggtctcg cgcgcgacat
     1861 caccggcagc atgcgagcgg gcatccttgc gttgattgtg ttcttcatcg tgggcagtgg
     1921 gctgctgctt ctcgtcaaca tgcaacgcgg gattgaacgc gcccgggcgg aggaagccgg
     1981 ataggacgtt tcgttacggt gttctttttc tggattcccg ccttcgcggg aatgacggca
     2041 cggttggcgg gggagcggca cgaatacctc gctgcccgga aggggccttg gatgacaact
     2101 taccatcctc cggcaggcca tggtggggca ggcaggctgc cctgtttcac acttccagct
     2161 tgttcccgaa cgccagcgat ggcacggctg cctgcggttc gccctccagc gtcaactcaa
     2221 ccaccgaagg caggttgtcg ggcgggtact ccgggaaccc gtgcagccag acgcggtcgc
     2281 ccttctgctc gatacgggcc tcctcgccgg tgatgagcag tttcgccttc agcaccctgt
     2341 tcgcgcacca cgcgaacgga accgttgacc ccggccagcg gaggatgatc agatacacct
     2401 tgcccccggc gtacgttccc caaccgagcg ccggcgcggt gacgggcgac ggctgcgcgc
     2461 cgtagatcgc tgcgccattg gtgcgcatcc atgcgcccac ctgtctcagt cggtccaccg
     2521 cctcgcgtgg gaaacgcccg tccggttccg ggccgacatt cagcaacagg ttgccgccgc
     2581 cggacgcgca tgtcgcgagg aggtaaatca gctggtttgc tgatttccag ttccggtcgc
     2641 cctccgcata cccccacgtc tcattcatcg tatagcacgc ttcccatgcg cgtttctccg
     2701 gcacgatggc attttccggc gtgccaaaat cctccggcgt gccggcgcgg ttgttgatca
     2761 ggataccggg ctgaagctcc cgcgccatgg cgttgagttc ttcgggacgc cacacaccgg
     2821 caggcaccgc catatcgtac caaaggatgt ccacttttcc gtagttggtc aggatctcgc
     2881 gcacctgcgc atgcgcctgc tccaccatcg gggcgtacac ctcatccgcc tgccgcggga
     2941 tgttgaacag cgcgccgggg aagcgccagt ccacgaggga gtagtagagc cccatgcgca
     3001 ttccggcttc gtggcaggcg tccgcgaatt cggcgatgaa gtctctcttc gccgcgcgtt
     3061 tcgtcgaggt gaaatcggac accttgctgt catacatgca gaacccgtcg tggtggcgcg
     3121 tcaccatcac catgtagcgc atgccggcgt cctgcgcgat gcgcacccat tcgcggggat
     3181 tgtagtgctc aggaacaaat cggtccgcga gtctggcgta ctcttcctgt ggtatgtgtt
     3241 cctggtaata aatccattcg ccgtggctga caagggaata cacgccgaag tggatgaaca
     3301 tgccgaaacg tgcttctttc caccatacaa ggcggtcggt gttggcaggg gctgtcattg
     3361 ggatctcctg tgagttcgat agggtctgtt cgcaaaaaaa ccattcggta gtgctcgcca
     3421 ttcgacgact ggcgctgtgc gttatcgccc cgcccactcc ttcactgcga ggaaccgttc
     3481 catcggaacc gggataccat cggcgaacgg gcacagcacg aagttcgcct tccggagccg
     3541 tggagtgatg agccgatcga gcgatggcgc gatttcttca acgtccccgg caacgaaaat
     3601 ggtgggatcg agtactccaa aaatgatggt gtcttccccc agcacgtcaa gcgcggcctc
     3661 ccagggcgtg tcgcccgtcg gcggcggggt gagggcgtgg attccgtcgc atccggtttg
     3721 cctgatcaga tcgagtatcc ggtgcacgtg cccgcacatg tggagcagcg cggttttccc
     3781 ggtcgccttc acggtctcca cgaaatcccg ctggtgcggc atgttgaact cgcggtacac
     3841 cgccgggctg atgtagaagg tgctcgtgtt ttcgaccagg gtgaccgact cccacggccc
     3901 ttcagcgagc aacttgaagg cttccagttc ccgctcgtgc atggtcctga cgagcgcgcg
     3961 catgtcgccc ggatggtccg ccagcaggta atagaagtgc tgcgtgccca tgtcctgttc
     4021 gagcagccgc ggaatcgtgg acgggcccca gaagcgggtg acaacaccgt attcgccgat
     4081 cagttcatcc agagccgcca gcgccggatg gtcgtggtac gggtgaaacg tgacgccttc
     4141 ccacatcgcg gtgtagatgc gcaccgcatc aatggaatcc accggatact tgatcgggtg
     4201 cccgttttgc gtgatccctg tgagttcgcc gtgaggcgtt ctccaccggg tggtgactct
     4261 ggcgccctcc tgctcgtacg acacggccac gctctccggc cactggtgca ccggagcgcg
     4321 caggtcgtgc ggcgtgttcc agccgttcag aaggaagata tcgcacccga ggtgcttgta
     4381 gaaatcaatc ccaccgttgc cgcgcaggtg ctccggcagg agggcgaggg tgttgttgtc
     4441 gaccagggtg gtccacgcaa ggcgatcctt cggttgtttc cgaagtatcg cgcgcagccg
     4501 ttcgcgtccc gtcacacggc atccccgtcc tgagcgttca ccccgaaacg agttcgacgc
     4561 aatcgagcag ctcttcgcgg aatgcggcaa ccatggcgtc gttgttcgtg tcgtccagca
     4621 catacggcgc ggtgccgaga tgcatctccg tgagcgactt atccgccatg cccggcagcg
     4681 ttacctcgac gaggtggcgg attttcgctt tcgccgcatc gctgacggaa gcatcggcga
     4741 gcttttcgcg cagggcgacg acgatacgcg aatcctccac gctctccctg acggcttccc
     4801 agcggcgcga ggaggttacc ggtccgtcgg ggctggtctg gtaaacaatc gggtattcag
     4861 cgtggacggg gttccacatg gattcgccat ggttgtagca ccagtagccg atgccggtcg
     4921 cgccgtagcg cacggcgaat accgcctgca tgcggaactc tcccacgatg ttgatcgcct
     4981 tggtgttcca tcccatgggg cgggcataca tgtaggcgca gttgtacgtc cacatggtct
     5041 tctttgtgtt ccacagatac cgcatcacag gtgtgtcgtc atcgagcatg aagaacgcgg
     5101 gcgaccagat tgcggcgatt tccaccagtt cctcgaacat cgggagatca ccgccgccgt
     5161 tcacgtaaaa cttgatcgtc ggccgcgcct tcagcaccag cttgccgaag gccacgtagc
     5221 gccgtacggc atcccacccg ttgccgccga cttcgtccca ggtgtagagc gcgacatgct
     5281 cctcgggaat gcccttcgcg gcgaggtgcg acatgacctg ctcgaagtaa tccgcaaggc
     5341 gggcaacgta ggagggatca tcctcgccga cgccgacctg cggtaacccc gtcagcagaa
     5401 ggaacacatc gtgcccttcg agcggcgcga ggaactgatc catggtggtg aaatccacct
     5461 ccaggcgcgt ttgtccgccc tgttccactg ccttcgcagg cggcagcccg gtcagaaaaa
     5521 ccgtcgcgcc gtgcgccagc aggtccggta cggtgtgctg gttgtacgac gcccacatgc
     5581 agagtcgcat cgcatcgtag ccgcccatct cgaatggcag cacccgcagc gcgatttcga
     5641 ccaccgtttc tttcccgccg gcggagaacg tcaccttcac ggtgtgctcg cccgcggtgg
     5701 ccttcgcggc gtccacatcg aaccagaact cccgcgtttc gaggcggggt atggcaaccg
     5761 attccacctc tccaagcgga atgatggggt cccacaccac tttcccggag tcggggtttg
     5821 tgggcacctg cgtcaccttg tgcggcgtga tgacgaggcc gccgggaacc tctcccaccg
     5881 tggtgcgcac cattacggtg tccggggaca cgttgaggag cttgatggac accggttcgt
     5941 gctcgccggt gacacaccgc ctgcggattg caaggggcaa aaacaacctg cccgggagct
     6001 ccgcatccac gttgaggttc tcccacatgc gcccttcgaa gggcagaaca gggcacccgg
     6061 aggcatccga aagaaccgtc ggagcgatcc ctgcaagcgc gagcgcacgc ttcgcccggg
     6121 cgttgagttt gccggtccgc gccaccactt cgggagaaaa cgctgacgcg gcgccggcaa
     6181 gctgcaacac cgcaaggttg ccggcctcct gctcaatcgc ggcacattcc tcgcgcagaa
     6241 ccgccgggaa gcccgcgtcc gtcggtactc ccgcaaccgc atcgcgcagc gcgaccaccg
     6301 ccttccgcgc gagtgcctga tcgttccgat agggcgaaag cgtcagcgcg cgcgaacccc
     6361 gggcaaggac ctctccatcg gctttcctcg cgcaccattc gaaccggtat ttccccggcg
     6421 cactgacggc caccggcatc ttcagcacac ccttttcccc ggcgatgatg ccggttgcgc
     6481 cctgccgcac gccatccggc gcgagcaccg tcgcctcggc ctcaagcggc tccttccccg
     6541 gcgcgggatt ggtgacgcgg aaggtgatct cgtcctgcgt cagcacggcg tcccacacga
     6601 ggttctccgg ttccatgcgc actgcttcga ccttctgcag cccgaaccac gatccggtgg
     6661 aacgcgggct cccgcggaag gcgtgccact cgacgtgcgt gtcggcgctg gccggcaccg
     6721 tgaagcagta aagccgcccc gtgtcgctgc cggtgatgcc gaataccagt ccgcgcgggc
     6781 catcggcgat tttcccgaac gcaggggtgg ttctgtgcgc acgccggctg tcgcactggt
     6841 gcgtgaagac gatttccccg gcctgattgt agacgaggat cttcccgagt tccgtgcaca
     6901 gcacatattc cagcgcgccg tcgccatcca catccacgat cgcgccggga gcgtaacaga
     6961 aggcctgcgt gtcaaaatcc cacaccacgc ggccgtcttc gtcaaagcgg aacaggcggc
     7021 ccgactgc
//
LOCUS       DAOTNG010000160         6996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9361_length_6996_cov_4.433511, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000160 DAOTNG010000000
VERSION     DAOTNG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9361_length_6996_cov_4.433511"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="P5179_08705"
     CDS             complement(<1..1676)
                     /locus_tag="P5179_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95334.1"
                     /translation="MIHFPQERAIVARQIANRLGEILALQYSSIQPVEGWEYVDVGTR
                     QDVAPVPQAGWQPYELGSWWGGQDVTCWFRARAEIPEPKTGERPVCLLNPGGESLVLA
                     DGEPLQGLDANRQEVVLPRRFWGKNVELVLQSYSSPSNSNTGRRGFSVAAVALRDELI
                     RSFHWDLKVAFDASNALDNESLTSRRILEHVEKCVKAVDLNSAGDRRAFRSHIASVRK
                     IFAAGMEEFASSVGMGNVVLAGQSHIDVAWTWPLRETRRKVARTFSTLLNYMDQYPDY
                     VYFQSQPQVYEWVKSHYPSLWSRIKEQVRAGRWEPTGASWVEQDTNIPSGESHVRQFL
                     YGNRFFRREFGVHTRVFWLPDCFGFSFALPQILRKAGVEAFATTKLASNEFNEFPYSL
                     FRWRGLDGSEVLAYQMPWEWNVTPANLLAHWNRFKQKTSTDEIPMTFGIGDGGGGPTA
                     EMLENAERLKNVRGFPQARAGRFQDHFDRLMTSVNREDLPVYVDELYYEKHRGCQTTQ
                     SRTKRNNRKSELALREAEFLASCELIAGGEYPQETISEAWKTVLLNQFHD"
     gene            2088..3566
                     /gene="glpK"
                     /locus_tag="P5179_08710"
     CDS             2088..3566
                     /gene="glpK"
                     /locus_tag="P5179_08710"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRS95335.1"
                     /translation="MVLAIDQGTTSSRAIIFDVSGAILGQGYAELPQYYPQPGWVEHD
                     PEEIWSTVTEAIKQALVASNVSPAEIKAVGVANQRETTIIWDRRTGKPIHRAIVWQCR
                     RTSDICDRLRAEGWEEEIHNATGLFIDPYFSATKIRWLLDNVEGAQEAAEKGHLAFGT
                     VDSWLLWKLSGGHIHATDYTNASRTLLFDIRAKSWNQKLLSHLKIPRSLMPQVKYSSV
                     MYGKTNCPDVLPKPVTISGVAGDQQASLFGQFAYQPGQTKTTYGTGCFLLQHTGAEPK
                     VSAHGLLATLAIDETGGPAYALEGAVFNAGSAVQWLRDGLQVITSSAESEAYAEQVSD
                     TLGVYVVPAFNGLGAPYWNASVRGAMFGITRGTARHHIIRATLESIAYQTADVLDALA
                     EDSGIRIPELFVDGKATENNFLMQFQADIAGVPVVRPVMQETTVLGAALLAGIGAGVW
                     SSAQQAQEIARLDRVFEPAMGEEQRHALRNGWRRAVAAATQF"
     gene            complement(3557..4219)
                     /gene="coaE"
                     /locus_tag="P5179_08715"
     CDS             complement(3557..4219)
                     /gene="coaE"
                     /locus_tag="P5179_08715"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRS95336.1"
                     /translation="MRYAVTPALGLTGGTGTGKSTVAKFLARRGARVIDADKIGHVVL
                     NTNKKVRSVLEDAFGADILGENGLIDRKKLGDLVFGDPVALARLNAVVHPPLLETLRE
                     ELDRARLDSALPLVVVDAALIVEWGITSWFDALVVVTAPRGIVEARLAAKGLDLDAIA
                     RRISSQLPDEERIKNAAMVVQNDGDLVHLEEAAGRIWDSFARAVPPEQSSAGGGTDAT
                     QN"
     gene            complement(4204..5082)
                     /locus_tag="P5179_08720"
     CDS             complement(4204..5082)
                     /locus_tag="P5179_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95337.1"
                     /translation="MSMSAKHIESLVARYLVTRIKTAPPEDVGKWANRISNTGWHVFS
                     RQRRVACANVARAFPELDEKAAIGMARASFRHAAVALVDAVRMPARLPDFVWTCPDVL
                     GPILEKKLPAIVISGHIGNWEVAGAAIATRGGRLHLIVATPTSPGVARLVHCLRTGWG
                     VIPHAREKSLLPLARALRRGELVGTAVDQWPARHAVPVTFLNQPTPFASGLFDYAIRT
                     GVPVVAIWAIRTNQNSATPEYAVTTELVWDGEEPVRSADDLVTRWVRMLERVIRCHPE
                     QYLWMHERWKNRCDTP"
     gene            complement(5057..6058)
                     /locus_tag="P5179_08725"
     CDS             complement(5057..6058)
                     /locus_tag="P5179_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HRS95338.1"
                     /translation="MVRWWRTNMDRTGLGAGQIAGLFLCAMIFIPEGAPASLPAQRRA
                     AGNWEVTRFGNDLVIEHKPHMRSSAERAAAIVQMARRRFYALADTSFPFEIRVLLASS
                     ATEFARLTRGIVPDWGAAAAVPDEGLMIISGLSEEKPMSEAIYHEVAHVALHALSRGP
                     VPRWFDEGIAMRLSDEWSIAYSVRLARSALANRLLPLHSVERVLSFEREQAALAYAVS
                     FAAVRWFEKRHGDRALALLLRSLASHSFDEAFVRATGSDSESFEREWLRSARNSYALV
                     GLADDMWIWSILIPGLFFLALGVRWWRNRRTLARWKREDRDSSGSDEPLDEHVSETY"
     gene            complement(6058..>6996)
                     /locus_tag="P5179_08730"
     CDS             complement(6058..>6996)
                     /locus_tag="P5179_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRS95339.1"
                     /translation="KTIVVIGFPARDTKHMPDGSKVDSLGGVLFTAIPLAVLSRRRGH
                     RVVPVSSFGVDCAAEGEDALRAAGCELEHVRVVQAHTQHSFITFTSDNERWERVTGKL
                     PALGEEDLHCVRGADCVVVNFITGAEMEYDTFRWLAEEYQGPIIMDYHTLALGTNPDG
                     RREPRRRTDWAAWFQHGTVVQMNEAEAGALAGHSLEDARACAAFCRDLLSLGPDAVVI
                     TRAEKGAVGAERVVRHGREAWATYEVPAPNVPHVVDTVGCGDVFLAGLAVGWIQWHAL
                     ETAMRFAAGVAALHVEYEGLTGAERLESVWEAFEGP"
BASE COUNT         1465 a         2176 c         1951 g         1404 t
ORIGIN      
        1 atgtcatgga actggttcag aagaacggtt ttccacgctt cggagatcgt ctcctgcggg
       61 tattcgccgc ccgcgatgag ttcacacgaa gccaaaaatt ccgcctcgcg caacgccagt
      121 tcacttttgc ggttattccg cttcgtccgg ctttgggtag tctggcaacc tcgatgcttt
      181 tcgtaataca gctcatccac atacacggga aggtcttcgc gatttaccga tgtcatcagc
      241 cgatcgaagt ggtcctgaaa cctacctgcc cgtgcttgcg gaaacccacg aacatttttg
      301 agacgctctg cgttttcgag catttccgcg gtggggccac caccgccatc tccgatcccg
      361 aaggtcatag gaatttcgtc ggtcgacgtt ttctgcttga agcggttcca atgcgcaagg
      421 agattggccg gggtgacatt ccactcccac ggcatctggt acgcaaggac ttccgatccg
      481 tccagtccgc gccaccgaaa caggctgtac ggaaactcgt tgaattcgtt cgacgccaat
      541 ttcgtcgttg cgaacgcttc gacacccgcc ttgcgtaata tctgcggcaa ggcaaacgag
      601 aaaccgaaac aatccggcaa ccagaaaacg cgagtgtgta caccaaattc gcgtcggaag
      661 aagcgattac cataaaggaa ctgccgcacg tgcgattcgc cactcgggat attcgtgtcc
      721 tgctcgaccc acgatgcccc cgtgggttcc cacctgccgg cgcgcacctg ttctttgata
      781 cgcgaccaga gagaagggta atgggatttg acccactcgt acacctgcgg ctggctctgg
      841 aaatagacat aatcggggta ctggtccatg taattcagca gggtggaaaa cgtccgcgcg
      901 actttccgcc gcgtttcccg aagaggccac gtccacgcca catcgatgtg ggattgcccg
      961 gcaagaacca catttcccat cccgacagac gacgcaaatt cctccatccc cgcggcgaag
     1021 attttcctta ccgacgcaat gtggctgcgg aacgctcgtc ggtcaccggc gctgttcaaa
     1081 tccacagcct tcacgcactt ttccacatgc tcgaggattc gtctgctggt gagcgattcg
     1141 ttgtcaagcg cgttggaagc gtcgaaggca accttcagat cccagtggaa ggaccttatc
     1201 aactcgtcgc ggagcgctac ggcggcaacg gagaaccccc gtcgcccggt attcgagttc
     1261 gacggcgatg agtacgactg gagaaccagt tccacgttct tgccccagaa tcggcgtggc
     1321 agcacgactt cctgcctgtt ggcgtccagg ccctgcaacg gttcgccgtc ggcgagaacg
     1381 agagactctc caccggggtt gagaaggcag acaggacgtt cgcccgtctt tggttcgggt
     1441 atctcagcac gggcgcgaaa ccagcacgta acgtcctgtc caccccacca cgaaccaagc
     1501 tcgtagggct gccaaccggc ttgtggtaca ggggcaacat cctgccgtgt accgacgtcg
     1561 acatattccc atccttcgac cggttggatg gaggagtact ggagcgcaag aatctccccg
     1621 agcctgttcg caatctgccg cgcgacgatg gcgcgctctt gaggaaagtg gatcatacgg
     1681 aggccgtttc ggttgtgctg aacgcatggt ccagcaaagc attaacgcga ggggtaggtt
     1741 ccttgaaagc cagagaagga aatggtgccc cgccacacag cgcaacaggg caaacgggtt
     1801 caaagccacc tgaacgaata tacgtgctac tcgacgcgga cggaatgctc cttcttcgat
     1861 gtcggacgag cgagcgcacg ttgtgaaaac ccgtctcaag cccgtataat tgagtgatgt
     1921 gttgcctcat gcggcgcggg ggcatgcctc actaccagaa ctctggatga ggctcgaacc
     1981 agttcccttt ttctgcctcc acttccgcac tggtttccgc gttcgccttt ttttgtttga
     2041 ctgaagctgt tcattgaccc gctaatcccg tatggggaga tagaagcatg gttcttgcga
     2101 ttgatcaggg aacaacaagc tcgcgtgcga tcattttcga cgtttcaggc gcaattctag
     2161 gacaaggtta cgccgaactt ccccagtatt atccccaacc cggatgggtg gaacacgacc
     2221 cggaggagat ctggtccact gttacggaag ccatcaagca agcgctagtt gcatcgaacg
     2281 tctcaccggc ggaaatcaaa gccgtagggg tcgcaaacca gcgcgaaacg accattatct
     2341 gggataggag aaccgggaaa ccgatccacc gggcaattgt gtggcagtgc cgacgaacgt
     2401 ctgatatctg cgacagactt cgcgccgagg gttgggaaga ggaaatccac aacgccacgg
     2461 gcctttttat cgacccgtac ttcagcgcaa cgaaaatccg gtggttgctg gacaacgtcg
     2521 agggtgctca ggaagcggcg gaaaaggggc acctggcctt tggaacggtc gattcctggc
     2581 tcctctggaa actcagcggc gggcatattc acgcgacaga ctacacaaat gcatcacgaa
     2641 cacttctttt tgacatccgc gcgaaaagct ggaaccagaa gcttctctcc catttgaaga
     2701 ttccgcgttc tcttatgccg caggttaaat actcctcggt gatgtacggc aaaacaaact
     2761 gcccggacgt gctcccgaaa ccagtcacga tcagcggcgt tgccggtgat cagcaggcat
     2821 cgttgttcgg ccagttcgcg taccagcccg gccagaccaa aacgacgtat ggcacgggtt
     2881 gctttctcct tcagcacacc ggggccgagc cgaaggtgtc cgcacatggc cttcttgcga
     2941 cactcgcgat agacgaaacg ggtggtccgg cttatgccct cgaaggcgct gtcttcaatg
     3001 cagggtctgc ggttcaatgg cttcgcgacg gtcttcaggt gatcacctcc tccgccgaat
     3061 cggaagcata cgcagaacag gtcagtgaca cacttggggt atacgtcgta ccggcgttca
     3121 atggcctcgg tgccccctac tggaacgcgt ccgttcgagg agcgatgttc ggcatcaccc
     3181 gcggaaccgc gcgccaccac atcatccggg caaccctcga atcgattgcc taccagacgg
     3241 cggatgttct ggatgcactg gctgaggaca gcggcattcg gattccagag ctttttgtgg
     3301 acggcaaagc caccgagaac aacttcctca tgcagtttca ggccgacatc gccggtgttc
     3361 ccgtagttcg tcctgtcatg caggaaacga cagtgctggg agcggcactt ctcgccggaa
     3421 taggcgcagg ggtgtggagc agcgcacaac aggcccagga gatcgcacgc cttgaccgcg
     3481 tgttcgaacc ggcgatggga gaggagcaga ggcacgcgtt gcggaacggc tggagacgtg
     3541 cggtagcggc ggcaactcag ttctgagttg cgtcggttcc cccgccggcg cttgactgct
     3601 cgggcggaac ggcgcgtgca aatgagtccc agattcggcc cgccgcctct tccaggtgga
     3661 cgaggtctcc gtcattctgc acgaccatgg ccgcgttttt aatccgctct tcatccggta
     3721 actgggaact gattctccgg gctattgcgt caagatccag gccttttgcc gcgagacgcg
     3781 cttccacgat acctcgcggc gcagtgacga caaccagcgc gtcgaaccaa ctcgtgatgc
     3841 cccattcgac aatgagcgcg gcgtcaacca cgacgagcgg gagcgcgctg tcgaggcgtg
     3901 cacggtcgag ttcttcgcgg agtgtttcca gcaggggtgg atgaacaacg gcgttcagtc
     3961 gcgcaagtgc caccgggtct ccgaacacca gatcacccag tttcttccgg tcaattaacc
     4021 cgttttcccc gagaatatcc gctccaaacg catcctccag caccgaacga accttcttgt
     4081 tcgtattgag cacaacgtgg ccgattttgt cggcatctat cacgcgtgca ccacgccttg
     4141 caaggaactt cgccaccgtg cttttccccg tcccggttcc cccggtcagg cccagggccg
     4201 gtgtcacggc gtatcgcatc gatttttcca tctttcgtgc atccagaggt actgttcagg
     4261 gtggcaacgg atgacgcgtt ccagcatgcg tacccagcgc gtgacgagat catctgcact
     4321 gcgcaccggc tcctcgccgt cccacaccaa ttcagtggtg actgcgtatt ccggcgtcgc
     4381 gctgttctga ttcgtccgta tcgcccatat ggcgaccacc ggcaccccgg tgcggatggc
     4441 gtaatcgaac aacccggacg caaacggggt tggttggttc agaaatgtta cgggaaccgc
     4501 atgccgggcg ggccactgat cgaccgcagt tccgacgagt tcaccgcgtc tgagggcgcg
     4561 agcgagcgga agaagcgact tttcccgcgc gtgcgggatg acgccccagc ctgtccgaag
     4621 gcaatgcacc aggcgggcaa ccccggggct cgttggtgtc gcgacgataa ggtggagcct
     4681 gcctcccctt gttgcgatcg ctgccccggc cacctcccag ttcccgatat ggccggaaat
     4741 cacaatcgcg ggaagtttct tttcgaggat gggtcccaga acgtccggac aggtccagac
     4801 aaaatccggc agccgtgccg gcatccgtac cgcgtcaacc aaggccaccg cggcgtgccg
     4861 aaacgacgca cgggccattc caatagcggc tttttcgtcg agttccggaa atgcccgcgc
     4921 cacattggca catgccactc gacgttgcct cgagaacacg tgccatccgg tattcgatat
     4981 cctgttcgcc catttcccga cgtcttccgg cggcgccgtt ttgatccgcg tcacgagata
     5041 cctcgcaaca aggctttcaa tatgtttcgc tgacatgctc atcgagaggc tcgtcggaac
     5101 ccgaactatc ccggtcttcc cttttccagc gcgccagcgt tcgccggttg cgccaccatc
     5161 gtactccaag ggccaggaag aagagcccag ggataagaat cgaccaaatc cacatgtcat
     5221 ccgccaatcc gacaagtgcg tacgaattcc gcgcggaccg taaccattcg cgttcaaacg
     5281 actcgctgtc tgaacccgtg gctcttacaa aggcctcatc aaaagagtgc gaggccagag
     5341 aacgaagcag caacgccagc gccctgtcgc cgtggcgttt ttcaaaccac cgaaccgccg
     5401 cgaaacttac ggcgtaggca agcgcggcct gctcccgttc gaaggaaagc acacgttcta
     5461 cggaatgcaa cggcaacaat cggttcgcca gagccgacct tgccagacgg acgctgtatg
     5521 cgatgctcca ttcgtcggag agccgcatgg cgatcccttc gtcgaaccac cgcggaacgg
     5581 ggccccggga aagagcgtgc aacgcaacat gcgccacttc atgatagatc gcttccgaca
     5641 tgggtttttc ttcagacaga ccggaaatga tcatcagccc ttcgtccgga accgccgcgg
     5701 cagcacccca gtcgggaacg atcccgcgcg tcagcctggc aaattccgtg gcggaggatg
     5761 cgagaagcac gcggatttca aacgggaaag aagtgtccgc aagtgcgtag aaacgtcttc
     5821 tcgccatctg gacgattgcc gccgcacgct ctgcagatga gcgcatgtga ggtttatgct
     5881 caatgacgag gtcgtttccg aaccgggtga cctcccagtt tcccgccgca cgcctctggg
     5941 ccggaagtga agcgggtgcg ccctctggga tgaagatcat tgcgcacagg aagaggccgg
     6001 caatctggcc ggctcccagg ccggttcggt ccatgtttgt ccgccaccat cgaaccatca
     6061 gggcccctca aaggcttccc agacggattc cagtcgttcc gctccggtca acccttcgta
     6121 ctcgacgtga agtgcggcca cgcctgccgc gaacctcatc gccgtttcca gcgcgtgcca
     6181 ctgaatccat cccaccgcca gtccggcaag aaagacatca ccacacccga cggtgtcgac
     6241 tacgtgtggg acattcggcg cgggtacttc gtacgtcgcc caagcctctc gcccgtgtct
     6301 caccacgcgt tcagcgccca ccgcaccctt ttccgcacga gtaatcacta ccgcgtccgg
     6361 ccccaaggac agaaggtcgc ggcagaatgc tgcacacgct ctggcgtctt ccagggagtg
     6421 cccggcaagt gctcccgctt cagcctcatt catctggact accgttccgt gctgaaacca
     6481 cgcggcccag tcggtccgtc tgcgaggttc cctgcggcca tccggattgg tacccagcgc
     6541 aagtgtgtgg taatccatga tgatcgggcc ctggtattcc tcggcgagcc aacggaatgt
     6601 gtcgtactcc atttccgccc cggtgatgaa attcacgacc acacagtccg ctccgcgaac
     6661 acaatggagg tcttcttctc ccagcgccgg cagttttcct gttacccgtt cccagcgctc
     6721 attatcactg gtgaacgtaa tgaaggagtg ctgggtgtgc gcctgcacca cacggacatg
     6781 ctccagttcg caaccggctg ctcggagcgc atcttctcct tctgctgcgc agtcgacccc
     6841 gaagctggac acgggaacaa cgcgatggcc gcgtctccga gagagaaccg ccaagggaat
     6901 cgcagtgaaa agcaccccgc cgagcgaatc cactttgctc ccgtccggca tgtgtttcgt
     6961 gtcgcgcgca ggaaaaccga tcacaacgat tgtttt
//
LOCUS       DAOTNG010000161         6978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9394_length_6978_cov_4.932110, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000161 DAOTNG010000000
VERSION     DAOTNG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_6978_cov_4.932110"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /locus_tag="P5179_08735"
     CDS             <1..2998
                     /locus_tag="P5179_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95340.1"
                     /translation="YWKGQWPEKRVPKGQATGGGFPGWMAEDDWFNGRWQIADTVLAA
                     ADKQWIFTLNPLNTREFAEMRDFAARFRHTLRIRLVSDKILPAIISWEAYTDSTWFRR
                     DIVVERETRGQSAEKESTWLVEVCNGHLASQKPSPKLADAAGEGCAKLLRQHKAGSEL
                     VRFGVWTTRNEDPTTRDHTVVTVRTGEATFSFAANDLDQGPIHLEDFGVLVSETRHKA
                     TLRQFRRTRPKTKTYYDRVFEMPEQTLARAWQDMPAKRSFYFVLGVEGRRQRFGVEPN
                     GDVFRSHERQWFEKMPGRETERVKEEPNAVRWSFGLPAEPPTQRVPLRGYLPVMTTSW
                     ETDGLRYEQTAFATLLDRSLDDTPIRGDDPVVAIVLVKATNTSNVEKDGTLTLRVAAG
                     DAQERLQLARHGYVRNQHGKLRCVITHRKGRLSRTTEGVHYSVRLRPSECTTIAFKLP
                     FFGLNEAELGLLEVIQPQSALQEVTDYWERRIAAGTRIVTPEPLIDDFYRAHITHLLI
                     NHERHPASAVSGGHELDIARVGSFRYGAYTNESVMQLSDLDRRGYSQEAANGYELFLH
                     FQGTVPLPGDFTSHRGVLYGAGGSEHGNYNQNHGWALWGLAEHYFLTRDRDWLHRVAG
                     KIVEACDWITEQRERTKRLDAEGRPVIEYGFLPAGVLEDIQDYWYWLSTNAYTWWGMD
                     RAAQALNDIHHPESGRLLREATLFKNDLVRGFTASLQRSAVVRLQDGTAVPHFPSHQH
                     LRGRSYGWIRETLEGAIHLLRCKLIPPESPMGTRVMKDYEDNLYLSEQFGYSGASLPD
                     RERYWFSRGGFSQQPNLLCSPLPYLFRNEIKHYLRAYFNAFAAGFHADTRMITEHPLP
                     HLGDWAGDHFKTSDEAQSAYWLRLMFVYEEAETLYFGYALPRYWLRPGNRISIQRART
                     YFGETSVSYEVSDSGRQIVARIAPPTSTPPSEFRIRFRHAEGKPIRSVQVNGASWEDF
                     DHEFVRVPATASPLEVVASFGGRGKR"
     gene            3078..>3913
                     /locus_tag="P5179_08740"
     CDS             3078..>3913
                     /locus_tag="P5179_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRS95341.1"
                     /translation="MAKRRNLEQVFEALEDGIVAVTKDGQIFHANTAAARLLGGNLRS
                     RAGENIDKILSERCGREVRLVQRAIGSGKPSSPERLQLRAGENGFFSVIARVQLLSDE
                     TGLLGAVVVLRDETEISRLRANLEGARGLENIVGASEPMQQVFQLVREVARTDATVLI
                     QGETGTGKELVARAIHQLSNRADKPLVTVNCAALPEGLLESELFGHVKGAFTGALSDR
                     LGRFEAADGGTLFLDEVAEIPPMIQVKLLRVLQERVFERVGENRPRRTDARLISATNK
                     NL"
     assembly_gap    3914..4013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4305..4727
                     /locus_tag="P5179_08745"
     CDS             4305..4727
                     /locus_tag="P5179_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRS95342.1"
                     /translation="MAHYAIKAEVNTSFVQAVAATKAALATEGFGVLTEIDVQATMKA
                     KLGKDMLPYVILGACNPPHAFNVISAEPDIGLMLPCNVIVYEKPDGAIVVAAINPEVA
                     MQPAGNDQLRPAAAIVRERLERVVRAVEGTGIRRGQNG"
     gene            4724..4894
                     /locus_tag="P5179_08750"
     CDS             4724..4894
                     /locus_tag="P5179_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRS95343.1"
                     /translation="MTHPELLKRVIGVLVGAVLGFAYYKFVGCSTGACPITSNPWIST
                     IYGGVIGLLITL"
     gene            4986..5963
                     /locus_tag="P5179_08755"
     CDS             4986..5963
                     /locus_tag="P5179_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95344.1"
                     /translation="MSDAAEPLEVSTKLFGWNGMTCQIPVHWDVTSVSGSAKSGYFAF
                     DDEQDRRLEIKYERAGRMRKPDLDRTLSGYFATLKKKAKSGQELRLDDNVRVSGVETM
                     PAEYEVRSYGWEADAITRGLIWHCSTCHRIVIAECRARKNRVNTKEMAQVLTSIRCHC
                     EGDTAPWAVFDFEVLVPREFELGLSRLQAGLISFSFSAKKRRLVVDRLGMGQVVMKHS
                     SLDTYVRDVHYKKLRKIRLRFQPVNWNGHEGFRFEGEHKRLYDWIPALGARIRAWRVS
                     DHIAGLAWFEEPTNRIYVIRAEGPDCMELAEKVAASARSRIAGEVPGNG"
     gene            complement(6023..>6978)
                     /locus_tag="P5179_08760"
     CDS             complement(6023..>6978)
                     /locus_tag="P5179_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRS95345.1"
                     /translation="YTYSAKDPSEHARWERGLRRKFVELLGGFPATKEPLSPEVSEIV
                     EFPDYVRERVVFNTRPFMSVPAYLLRPNKPISGKLPAIVCLPGHGPGKNTIVGFDDKG
                     EPRQEIGGYQADFAIQAVRHGYVALAIEQLAFGERNSPDPRCRGCNEPSMNALMLGLT
                     MIGLRVWDARRALDYLLTRPEVDPGRIGVMGISGGGTTTLYTAAVDKRFKAAVVSGYL
                     CTFRDSIMAMDHCCDNFIPRIIRYAEMYDLAALVAPRALFAESGTKDSIFPIDAARFA
                     FRKAKRAWDVLGAPDKIGHEVFEGEHQFWGKGAFEFLKRNL"
BASE COUNT         1507 a         1897 c         2104 g         1370 t
ORIGIN      
        1 gtactggaag ggccaatggc cggaaaaacg cgtgccgaag gggcaggcga cgggaggcgg
       61 attccccggg tggatggccg aggacgactg gttcaacggc cggtggcaga ttgccgatac
      121 ggttctcgcc gctgccgaca agcaatggat tttcacgctc aacccgctga atacgcgcga
      181 atttgcggaa atgagggatt ttgcggctcg atttcggcac acactgcgta ttcgtctcgt
      241 gtccgacaaa attctacctg cgatcatttc ctgggaggcg tacaccgatt caacgtggtt
      301 tcgcagggac atcgtcgtag aacgtgaaac gcgcgggcaa tcagcggaaa aagagagcac
      361 atggcttgtc gaggtttgta acggacacct cgccagccag aagccttcgc ccaaacttgc
      421 tgacgcagcc ggcgagggct gtgccaaact tctgagacaa cacaaagctg gttctgaact
      481 ggtccgattt ggtgtatgga ccaccagaaa cgaagacccc acgacccgcg accacacggt
      541 agtgaccgtc cgcacgggcg aagcgacatt ttcgtttgca gccaacgatc ttgaccaggg
      601 gccgattcat ctcgaggatt ttggcgttct ggtgtctgaa acgcggcaca aagcgacgtt
      661 gcggcaattc cggcgtacac ggccaaaaac caagacatat tatgaccgtg tgttcgagat
      721 gcccgagcag acacttgcgc gtgcgtggca ggatatgccg gcgaaaagat ccttctactt
      781 cgtgttgggt gttgaggggc gcaggcagcg tttcggcgtg gagccgaatg gagatgtgtt
      841 ccgttcgcac gagaggcagt ggtttgagaa aatgccgggg cgtgaaacgg agcgggtcaa
      901 agaggaaccg aatgccgtga ggtggtcctt tggtctccct gcagaaccgc ctacgcagcg
      961 cgtgccgcta cgcggttacc ttccggtgat gacgacgtca tgggaaactg atggactgcg
     1021 gtacgaacag acggcgttcg ccactctcct cgatcgttca ctggacgaca caccgatacg
     1081 cggagatgac ccggttgttg cgatcgtcct cgtgaaggct accaacacct cgaacgtgga
     1141 aaaggacgga accctcactc tccgggtcgc agcgggcgac gcacaggaac gactgcagct
     1201 tgcacgccac ggctatgtga ggaatcaaca cgggaaactc cggtgtgtga tcacgcaccg
     1261 gaaaggcagg ctttccagaa ccacggaagg ggtccactat tcggtgcgtc tgcggccttc
     1321 ggaatgcact accatcgcgt tcaagctccc ctttttcgga ctgaatgagg cggaactcgg
     1381 tttgttggag gtgatccagc cgcagagcgc gttgcaggag gttaccgact actgggaacg
     1441 gaggatcgct gctggaaccc ggattgtaac gcctgagccc cttatcgatg acttctaccg
     1501 ggcacacatc acacaccttc tgatcaatca cgagcgtcac cccgcgtcgg ccgtttccgg
     1561 gggacacgaa ctcgatattg cgcgtgtcgg ctctttccgg tacggggcat acacgaacga
     1621 gtcggtcatg cagctatccg atcttgaccg acgcggttac tctcaggaag ctgcgaatgg
     1681 gtacgagcta ttcctccatt ttcagggaac cgttcctctt ccgggcgatt tcacgtcgca
     1741 tcgcggcgtg ttgtacgggg cgggaggcag cgaacacggg aactacaacc agaatcatgg
     1801 atgggctctc tggggacttg cggagcacta cttcctgaca cgagatcgcg actggctcca
     1861 ccgtgtggcg ggcaagatag tggaagcatg cgactggatt accgaacagc gcgaacgaac
     1921 aaagcggttg gatgccgaag gcagacccgt cattgagtac gggtttctgc ctgcaggcgt
     1981 tctcgaggac atccaggatt actggtactg gctatccacc aacgcctaca cgtggtgggg
     2041 catggacagg gcggcacagg cactgaacga catacaccat ccggagagtg gtcgacttct
     2101 tcgcgaagca acgcttttca aaaacgacct cgtcagaggt ttcaccgctt cactgcagcg
     2161 atctgctgtg gtccggttgc aggatggaac tgcggttccc catttccctt cgcatcaaca
     2221 tctgcgtggt cgctcgtacg gctggatcag ggagactctc gagggcgcaa tccatctgct
     2281 gcgctgcaaa ctgattccgc ccgagtctcc gatgggcacg cgggtgatga aggattacga
     2341 ggacaatctt tacctttcgg agcagtttgg ttacagcggc gcaagcctgc ctgatcgtga
     2401 acggtactgg ttcagccgcg gtggattcag ccagcagccc aatctgctgt gcagcccgct
     2461 gccctatctc tttagaaatg agatcaaaca ttacctgagg gcgtacttca atgcattcgc
     2521 ggcgggattc cacgccgaca cgagaatgat taccgagcat ccgttgcccc atctcggtga
     2581 ttgggcaggc gaccatttca aaacctctga cgaggctcaa agcgcgtact ggcttcgtct
     2641 catgtttgtt tacgaagagg cggaaaccct gtatttcggc tacgcgctgc cgcgttactg
     2701 gctcagaccc ggtaatcgca tttcgatcca acgcgcccgt acctacttcg gagaaacgag
     2761 tgtatcatac gaagtcagcg acagtgggag gcaaatcgtg gcaagaatag cgcctccaac
     2821 gagcacgccg ccgtcggaat tccgcatccg tttccgccac gctgaaggga agcccatacg
     2881 ttctgttcag gtgaatggcg cgtcgtggga ggattttgac cacgagtttg tgcgtgtgcc
     2941 cgccacggcg tcccctctgg aggttgttgc gtcgtttggc ggccgcggga agcgttgaac
     3001 acagaactca tcgttggaga tctggtttgc agaccccgaa caacgtgttg ccgatcggta
     3061 atgccctcga aacgtgcatg gcaaagcgcc ggaacctgga acaggttttc gaggctctcg
     3121 aagacggcat cgtggcagtt acgaaggatg gccagatttt tcacgcgaat accgccgcgg
     3181 ccaggttgct tggcggcaat ttgagatcgc gcgccggcga gaacattgac aaaatccttt
     3241 cggaacgctg cgggagagag gtccggctgg tgcagcgagc aattggttcc gggaagccat
     3301 cttcgccgga acgattgcag ctcagggctg gagagaacgg tttcttctcg gtaatcgcac
     3361 gcgtgcaact gctttccgac gagacgggac ttctcggtgc agtggtcgta ttgcgcgacg
     3421 aaacggagat ctcgcgtctc agggcaaatc tcgagggcgc gcggggtctt gaaaacattg
     3481 tcggcgcaag cgaaccgatg cagcaggtgt tccagcttgt ccgtgaggta gcccgtaccg
     3541 acgccaccgt gctcattcag ggcgagacgg gtaccggtaa agagctcgtg gcgagggcta
     3601 tccatcaact gagcaatcgc gcggacaagc cgctggtaac tgtcaactgc gcggcattgc
     3661 cagaagggtt gctggaaagc gagctgttcg gccatgtcaa aggcgcgttc accggcgcgt
     3721 tgagcgaccg tctgggaaga ttcgaagcag cagacggcgg caccctgttc ctcgacgagg
     3781 ttgcagaaat tcccccgatg atacaggtga agctgctcag agtcctccag gagcgcgtgt
     3841 tcgagcgggt gggcgagaac aggccacgtc ggacggatgc acgactgatt tcagcaacga
     3901 acaagaacct gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatccgaa
     4021 tggcgttgga gaaacatcgt tacaaccgca ccgcggcggc aaaagaactg ggcataagcc
     4081 ggataacgct ttggaggcgg atgcgggact gggacatcgc gccgcggaga tgattacttc
     4141 cacaacgcgt gcaccggagc gaacccgagt cggaagcggc ggtcacacga gacagcaggc
     4201 ggcagtaaaa ccccgacgtc ggaaccaacg tcgctggtgc atcagtagta tcaatgacca
     4261 agaaggtgtt acgtgttccc gcacaatgaa aggtgtgaag ccggatggcg cactacgcaa
     4321 tcaaggcaga ggtgaatact tccttcgtgc aagcggttgc tgcaacgaag gcagcacttg
     4381 cgacggaagg gtttggtgtt ctcacggaaa tcgacgtcca ggcaacgatg aaagcgaaac
     4441 tcgggaagga catgttgccc tacgtgattc tcggggcctg caaccctccc cacgcattca
     4501 atgtgatatc cgcggaaccc gatatcggat tgatgttgcc gtgcaacgtg atcgtgtatg
     4561 aaaaaccaga tggcgcgatc gtggtcgcag ccatcaatcc cgaagtggcg atgcagcctg
     4621 cggggaacga ccaattgaga cctgctgcgg caatcgtgcg tgaacggctc gaacgggttg
     4681 ttcgtgccgt ggaaggcacc ggcatccgca gggggcagaa cggatgaccc atccggaact
     4741 cctcaagcgt gtgataggtg tcctcgtcgg tgccgtactc gggttcgcgt attacaaatt
     4801 cgtcggttgt tcgacgggcg cgtgtccgat cacgtcaaac ccgtggataa gcacgatata
     4861 cggcggagtc atcggtctgc tcatcacgct ttgacagcgc gggactggca acaaagatcc
     4921 gtgggctgat tgcgcgccgc agtttttgcg ctggccgaac caaagagggg actatcagga
     4981 tcggaatgag cgacgccgct gaaccactcg aggtatcgac gaagcttttc ggttggaacg
     5041 gaatgacttg ccagatccct gtccactggg atgttacaag cgtttccgga tcagcgaaaa
     5101 gcgggtattt cgccttcgat gacgaacagg accggcgcct ggaaatcaag tacgagcgcg
     5161 cgggccggat gaggaaacct gatctcgata gaacattgtc gggatacttc gcaacgttga
     5221 agaaaaaggc gaagtccggt caggagttgc gtctggacga caatgtccgc gtttccggcg
     5281 tagaaaccat gccggccgag tacgaagtac gttcgtacgg gtgggaggca gatgccatca
     5341 cgcggggatt gatatggcat tgttcgacat gtcatcgaat tgtgatcgct gagtgccgcg
     5401 cgagaaaaaa ccgcgtcaat acgaaagaga tggcgcaggt gctgacgtcc atccgttgcc
     5461 attgcgaagg tgataccgcg ccctgggcgg tgtttgattt cgaggttctt gtgccccgcg
     5521 agttcgaatt aggcttatcg cgcctgcagg caggcctcat ttcgttcagt ttttcggcga
     5581 agaagcgtcg cctggtcgta gatcggctag gcatgggcca ggtggtcatg aaacattcgt
     5641 ctcttgatac gtacgttcgt gacgtccact acaagaagct gcgaaagatc cgactgcgtt
     5701 ttcaaccggt taactggaac ggccacgagg gattccgctt tgaaggtgag cacaagcgcc
     5761 tgtacgactg gatacccgca ctgggcgcga gaatacgggc atggcgtgtc tcggaccaca
     5821 tcgctggatt ggcgtggttc gaggaaccca cgaaccggat ctacgtaatc cgggcggaag
     5881 gccctgattg catggaactg gcggaaaaag tggctgcatc agcgcgaagc cggattgccg
     5941 gggaagttcc ggggaacggg taagccgctt gcgggtgggt ggagcctcct ccacccacct
     6001 caggcgcgtg aggaagcgga tttcacaggt ttcgtttgag gaattcgaac gctcccttgc
     6061 cccagaactg atgctcgcct tcgaacacct catgcccgat tttgtccggg gcacccagga
     6121 catcccacgc ccgctttgcc ttacggaatg cgaaacgcgc agcgtcgatg gggaaaatgg
     6181 agtcctttgt tccgctttcg gcaaaaagcg cccggggcgc taccagcgcc gcaaggtcgt
     6241 acatttccgc gtagcggatg atgcgcggga tgaagttatc acagcagtga tccatcgcca
     6301 ttatgctatc ccggaaggtg caaaggtacc cactgaccac tgccgctttg aacctcttgt
     6361 caaccgccgc ggtatagagc gtggtggtgc cgcctcccga tatccccata accccgatac
     6421 gtcccggatc tacttccgga cgcgtgagaa ggtaatccag cgccctccgg gcatcccaaa
     6481 ctctgagccc gatcatcgtc agcccgagca tgagcgcgtt catggacggc tcgttgcacc
     6541 cacggcaacg cgggtccggt gaattcctct cgccaaacgc cagttgttcg attgccagcg
     6601 caacataccc gtgacgcacg gcctggatcg cgaaatccgc ctggtagcca ccgatctcct
     6661 ggcggggttc tcccttgtca tcgaaaccga cgatggtgtt cttccccggc ccgtgcccgg
     6721 gcagacacac gattgcgggg agtttcccgc tgatcggctt gttcggacgt aaaaggtagg
     6781 ccggcaccga catgaacggc cgtgtgttga agacgacgcg ctcgcggacg taatcgggga
     6841 actccacaat ctccgacacc tccggcgaaa gcggctcctt cgtggcgggg aaccctccga
     6901 gcagttccac aaacttcctg cgtagaccgc gttcccaccg cgcatgttcc gacgggtcct
     6961 tggccgagta cgtgtaca
//
LOCUS       DAOTNG010000162         6972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9409_length_6972_cov_5.778950, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000162 DAOTNG010000000
VERSION     DAOTNG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9409_length_6972_cov_5.778950"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1353
                     /locus_tag="P5179_08765"
     CDS             163..1353
                     /locus_tag="P5179_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRS95346.1"
                     /translation="MRESFSIPLITLFGFLLLACATETYATGTLGQLDEQRLRQTVDS
                     LYGSPYRYGGRSREGFDCSGFVGTVFQDQGIELPRSSREMYRVGDPVKRNQLAVGDLV
                     FFRTRGRRRGVSHVGIVNGPDSFVHANTRRGVITERLTDPYWKARYVGARRVATPVSP
                     PPAQEIAEDSYPFNTSSLVNTPTIALCPGKGLSALSFATGFRGDVAISPEFTFMNRLQ
                     AGASVRLRRAVGTGTPSIAVPSIRAKLRINEQIGQIPGFAIGFDSERDELTYSTALGD
                     STASSPRRGLFAVGSGTLVYPRSFFLGRGTGHAGASLSSFAGNGIKRNLSFFMGVDQQ
                     LLQRMTLLGEIDNIMGVGGWRANVGARFSITDDAVLEYAVLHMAGDNIRPDKVLKFSF
                     SVPY"
     gene            complement(1431..4517)
                     /locus_tag="P5179_08770"
     CDS             complement(1431..4517)
                     /locus_tag="P5179_08770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRS95347.1"
                     /translation="MSYAALWCKSNFSFLEGASHPSELVEQAATLGLHAIALTDRDGV
                     YGAVQALLAAESTGARLIIGSEISLDDGSTIILLCRTRKGYSNLCRLISAGRMRSPKG
                     ESKVSWEEVCSHSEGLLALWGGDRSLLLRKADPDIVARQLKEAFHDHLHVIISRHRRA
                     DDVAKEALLRLRARKYDLPTLAVPEVLYHEVSRRPLQDVMTCIRHGVTLSSAGRRLKP
                     NAEYDLKSPARMKRLYRDDLESLERTCAVADSCTFTMREIAYRYPSGQLPNGMTGEEW
                     LRKITYEGAAQRYPDGIPDEVTLQVEKELSLITELKYTGYFVTMWEIVRYCRREGILC
                     QGRGSAANSAVCFCLGITAIDPVRMGLLFERFISRERAEPPDIDLDIMHERREEVIQH
                     VYDEYGRSRAAMVANVVRYRAKRAIREVGKVLGMAESACDRLAKLTSSYGDLTDPALR
                     DAGLDPDQPLHRHLLTISNEIIGMPRHLSIHPGGFLLGAEEILDIVPVENATMEKRTV
                     IQWDKDDVEALGLFKLDLLGLGALTQLDICFRLLASHRGVNLSMATIPADDRATFDMI
                     ARGDTVGVFQIESRAQQAMLPRLRPRNYYDLVIEIAIIRPGPITGGMVHPYLRRRNGE
                     EAVIYPHPCLEPVLQRTLGIPLFQEQVMKLAMVAADYSPGEADQLRRDMAAWKKKGGL
                     ERHHDRLVNRMREKGIDKAFAERVFEQIKGFGEYGFPESHSASFALISYASAWLRCHY
                     PAEFLCSILNAQPMGFYSPATLVQDAIRHGVSVRPVDVSWSSWDCTLEPLPDGEFAVR
                     MGLRHVNGLGKKDAETIQNARINGPFSDIDDFVRRTSLDEGVLRSLAQAGGFESLERH
                     RRQAVWRVKGASRTDTLPLAVAEPDPEPVFELLSEADKTAWDYRASGHSTRAHPLLAL
                     RPLLSAQGILSASEIRALQDRSLVRYAGLVICRQRPGNANGVTFMTLEDETGHANLII
                     WKDIYQRHRILAKTTSFLGVTGEIQSRSGITHLIARRLWVPRVTARPEVVKSRDFH"
     gene            complement(4517..5965)
                     /locus_tag="P5179_08775"
     CDS             complement(4517..5965)
                     /locus_tag="P5179_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95348.1"
                     /translation="MSRLACVWIADLPLQILCLRHSEWSGKPVAVITRDAPQAEILYM
                     NPVARKMGVRTGMRYAAALGLAPQLHASTVSAEDLAGCIALLSRRMRTFTPDVEPDAG
                     EPGVFHLNAGGLTGLFGSETAWSKEVWKAVRSEGFVSSIAVGFTRFGTYAAAKTRKGE
                     SVFPSPELERKAALAVPLHALGLPPDTTQELSLLGVNTVDNLLHLPVYGLRLRYGEQV
                     FCLYKSACGDLWQPLQADAEKPLHARRMIFDTPERDTQRLCRFLEKMLTTLAGEVEKK
                     GELIRSIHWTCTLDTGLPYSSEVTPSNPTGNIQRLMELIHLRIEAGPPPSAVEEIVLS
                     VQSSPAYTAKRDLFDVPEPRNRVAGERALARIRAAFGDDAVVTAHLADEHLPERSFHW
                     DIARSVPVPNPCDVSEGFPYMRRIYTVPQRLPDEYRQTGNRVVGEYRISGDWWSRKTV
                     REYRFILTNEGRLLWVYYDYSRADWFIQGEVE"
     gene            complement(5987..6673)
                     /locus_tag="P5179_08780"
     CDS             complement(5987..6673)
                     /locus_tag="P5179_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HRS95349.1"
                     /translation="MVISAVQDMLREIPIKSASALHIHADRSEWRYKELRGRLTEISA
                     WENPAALSSVMMLVRQAQCAGEPTAWVGKASDIFFPLDAAAYGVDLAALIVVRLSQLS
                     DTPRAADMLIRSGGFGLVVIDLSDASPVCIPMPLLSRIVRLSHVKNVATVCLTRKPVH
                     ASSLGSLISLRTQARRVGKFDATAGISLEAIKDKQHAPGWVHREYRGVPVGMRSDNRF
                     AAPIALCKAS"
BASE COUNT         1515 a         2066 c         1941 g         1450 t
ORIGIN      
        1 atccttctcg gacatgaaga catctctacc actcagatat acacgcatgt agaccgggag
       61 catctgaagg caatgcaccg ccaattccat ccgcgtggtt gaaaccacgg cgcgcggtta
      121 tgggctccgc atatcgtaac cggcgtcaaa acggagtgta atatgcgaga atcgttttca
      181 atccctctga taactctctt cggttttctc cttctcgcgt gcgcgactga aacctacgcc
      241 accggcaccc tcggccagct tgacgagcag cgactgaggc agacagtgga ttcgctgtac
      301 gggtctccgt atcgatacgg cggcagatcg cgggaaggtt ttgattgttc cggttttgta
      361 gggacggtgt tccaagacca gggcatagaa cttccgcgca gctcgagaga gatgtaccgg
      421 gtgggagatc cggtcaaaag aaaccagctc gcggtcggcg atctggtttt tttcagaacg
      481 cgtggaagaa ggcgaggggt cagccacgtg ggtatcgtca acgggccgga ttcattcgtg
      541 cacgcgaaca cgagacgagg cgtcatcacg gagagactga cggacccgta ctggaaagcg
      601 cgttacgtcg gcgcacgacg ggtggctact cctgtcagtc cgcctcccgc tcaggaaatt
      661 gccgaagaca gctatccgtt caacacctcc tcgctcgtca atacccccac gatcgcgctc
      721 tgcccgggga aaggactttc tgcgctgagt tttgcaaccg gctttcgcgg ggatgtggcc
      781 atcagtccgg agttcacctt catgaaccgc cttcaggcag gcgcttctgt tcgcctccga
      841 agagcggtag ggacgggtac gccttcgatt gcggtgccaa gtatcagagc gaagctccgc
      901 atcaacgaac agatcgggca gatacccggt ttcgcgatcg ggtttgactc ggagagagac
      961 gaactcacct attccacggc gttgggtgat tccacagctt cctcgccccg ccgggggttg
     1021 ttcgcggtgg gaagcggaac acttgtctac cccaggagct ttttcctggg gcgtggaacc
     1081 gggcacgcag gtgcaagcct ttcatcattc gccggcaacg gcatcaaacg gaacctttcc
     1141 tttttcatgg gggtagatca gcagcttctc cagcgcatga cgctcctcgg ggaaattgac
     1201 aacatcatgg gcgtgggcgg ttggcgagcg aacgtcggcg cccgtttcag catcactgat
     1261 gatgcagtac tcgaatacgc ggtgctccac atggccggag acaacatccg ccccgacaaa
     1321 gtcctcaagt tcagtttcag cgtgccctac tgatcgtttc cccgtcacct ctgtttttct
     1381 gccttttgcg cgcaactcgg acgcactttg cccgtcaacg ggatcgtaca tcaatggaag
     1441 tcgcgacttt tcaccacctc cggcctggca gtgacacgcg gcacccataa ccggcgtgca
     1501 atgaggtggg taataccact cctcgattga atctctcccg taacacccag aaatgacgta
     1561 gtcttggcaa gaattcgatg ccgctggtag atgtccttcc agatgatcag gttcgcgtgg
     1621 cctgtctcat cttcgagcgt cataaaggtc acaccgttgg catttcccgg acgttgcctg
     1681 cagatgacga gaccggcgta cctgaccaga gaacgatctt gaagcgcgcg gatttcggag
     1741 gcactcagta tcccctgagc ggagagcaaa ggcctgagcg ccagcagggg atgtgcccgt
     1801 gtgctgtgcc cggatgctcg gtaatcccac gcggtcttgt ccgcttcaga cagaagctca
     1861 aataccggtt ccgggtcggg ttctgccacg gcaagaggca atgtgtcggt acgtgaagct
     1921 cccttgacac gccacactgc ctgacgccgg tgccgttcga gactctcaaa cccgcctgcc
     1981 tgtgcgagag aacgcagaac gccctcgtcc agactggtgc gccggacaaa atcatcgata
     2041 tcagagaaag ggccgttgat acgcgcattc tgaatcgttt ctgcatcctt cttcccgagc
     2101 ccgttcacgt gcctgagccc cattcgaact gcaaattccc catcaggcaa gggttccagc
     2161 gtgcaatccc atgagctcca cgacacatcc acaggtctga cggacacacc gtgccgtatg
     2221 gcgtcctgaa ccagtgttgc agggctgtag aaacccatcg gttgcgcgtt gaggatggag
     2281 cacaggaact ccgcgggata gtggcacctc aaccatgcag acgcgtagga aatcagcgcg
     2341 aaacttgcac tgtgggattc cggaaaaccg tattcaccaa atccctttat ctgctcgaaa
     2401 acccgctcgg caaatgcttt gtcgatcccc ttttctctca tccggttcac aagacgatcg
     2461 tggtgccgtt ccagcccgcc ctttttcttc cacgcggcca tgtcgcgccg caactggtcc
     2521 gcctcgccgg gggaatagtc agccgccacc attgcaagtt tcatcacctg ttcctggaac
     2581 agagggatgc cgagcgtcct ttgaagcacg ggttcaaggc agggatgagg gtagataact
     2641 gcctcttcgc cattgcggcg ccgcaggtac gggtgcacca tccctccggt aatcggaccc
     2701 ggccgaatga tggcaatttc gatcacgaga tcgtagtagt tccggggacg cagacgtgga
     2761 agcatcgcct gttgcgcacg actttctatc tggaacacgc cgacagtatc accccgggca
     2821 atcatgtcga aggttgcccg atcatccgcc ggtatcgtcg ccatggagag attcacgccc
     2881 cggtgggatg ccagcagacg aaaacagata tccagttgtg tcagagctcc gagcccgaga
     2941 agatcgagtt tgaacaggcc cagcgcctcc acatcgtcct tatcccattg aattacggtg
     3001 cgcttctcca tagtagcgtt ttcgaccgga acaatgtcga gaatctcctc tgcaccgagc
     3061 aggaagccac cgggatgaat ggacagatgc cggggcattc cgatgatttc attggagata
     3121 gtcagaaggt gccggtgaag tggctgatcg ggatccaaac cggcgtcacg cagggctgga
     3181 tcggtgaggt cgccgtacga tgacgtaagt tttgccagac ggtcacacgc actctccgcc
     3241 attccgagca ctttgcccac ttcccggatc gcccgttttg cccggtatcg tactacgttt
     3301 gctaccatgg ccgctctgga tcgcccgtat tcgtcataaa cgtgttgaat aacctcttct
     3361 ctgcgctcgt gcataatgtc gagatcgatg tcaggcggct cggcacgctc gcggctgatg
     3421 aaccgctcga aaagaagccc catacgcacc gggtctatcg cagtaatacc gagacagaag
     3481 cagaccgcgg aattcgcggc tgacccgcgc ccctggcaga gaattccttc gcgcctgcaa
     3541 taccgaacaa tctcccacat cgttacaaag tagccggtgt atttgagttc cgtgataagg
     3601 ctcagttctt tctccacttg cagggtgact tcatcaggaa tgccgtcggg ataacgctgg
     3661 gctgcccctt catacgtgat ttttctcaac cactcttcgc ccgtcatacc gttgggcaat
     3721 tggcctgaag ggtaacgata ggcaatctcg cgcatggtga atgtgcagga atcagccacg
     3781 gcgcatgttc gttcaaggga ttcgagatcg tcgcggtaga ggcgcttcat tcgcgctggt
     3841 gatttcaggt cgtactcggc gttcggcttc agccgccttc ctgctgagga aagcgtcact
     3901 ccatgccgga tacacgtcat tacatcctgc aacgggcggc gcgacacttc gtggtaaagc
     3961 acttccggta ccgcaagcgt cggcaggtcg tacttccgcg cacgaagacg cagaagcgct
     4021 tcctttgcaa cgtcatcagc cctccgatgg cgggagatga tcacatggag gtggtcatga
     4081 aaggcttctt tgagttggcg ggctacgata tcgggatccg ccttgcgcag gagcaggctt
     4141 cgatctccac cccagagagc cagaaggcct tccgaatgcg agcagacctc ctcccatgaa
     4201 accttgctct caccttttgg cgagcgcatc ctccccgcgc tgatgagtcg acagaggttc
     4261 gagtagcctt tccttgtgcg gcagagaaga atgatcgttg aaccgtcatc cagcgaaatc
     4321 tcagacccga ttatgagccg tgctccagtg gattctgctg ccagaagagc ctgtaccgcg
     4381 ccatatacac cgtccctgtc ggtgagagca atcgcatgca atccgagtgt tgcagcctgc
     4441 tctaccagtt ccgatggatg cgacgccccc tcaaggaacg agaaattgct tttgcaccag
     4501 agagctgcat acgacactat tcaacctctc cctggatgaa ccagtcggcg cgggaatagt
     4561 cataatagac ccacagcagg cgcccttcgt ttgtcagtat gaaccggtac tcccgcacgg
     4621 tctttctcga ccaccagtcg ccagaaatac ggtattctcc caccacgcga ttacccgttt
     4681 gacggtattc atcgggaagg cgctgcggca ctgtgtagat acgccgcatg taaggaaatc
     4741 cctcggatac atcgcaagga ttcggaacgg ggacggagcg ggcgatgtcc cagtgaaacg
     4801 agcgttccgg caggtgttca tcggcgagat gagctgttac cacggcatca tcgccgaacg
     4861 ccgcgcgaat acgtgcaagc gctcgttctc cggctacgcg gtttcgaggc tccggcacgt
     4921 cgaacagatc cctctttgcc gtataggcag gtgatgactg aaccgagaga acgatctcct
     4981 caactgccga gggaggaggc ccggcttcga tacggagatg aataagctcc ataagccgct
     5041 ggatgttgcc tgtggggtta ctcggcgtga cttccgacga atacggcagg ccggtatcaa
     5101 gtgtgcaggt ccagtggata ctccgtatga gttctccttt tttctccact tcgcctgcga
     5161 gtgtcgtaag catcttctcc agaaaccgac aaagccgctg agtgtcacgt tccggtgtat
     5221 cgaatatcat acgccgcgca tggagcggtt tttccgcatc tgcctgaaga ggctgccaga
     5281 ggtctccaca cgcgcttttg tacaggcaga aaacctgctc gccataccgc aagcgcaagc
     5341 cgtacacagg cagatggagc agattgtcta cggtgtttac gccgagcagg ctgagttcct
     5401 gagtagtgtc aggaggtagc cccagcgcgt gaagcggaac ggcgagcgcc gcctttcgct
     5461 cgagttccgg cgaagggaaa acactctccc ctttccgcgt tttcgccgca gcgtacgtac
     5521 caaaccgagt aaaccccacc gcgattgagc tgacaaatcc ctccgagcgg acagccttcc
     5581 acacttcctt cgaccatgcg gtttccgagc caaacagccc ggtgagaccg cctgcgttga
     5641 ggtgaaacac acccggctcc ccggcgtccg gctccacgtc cggggtaaac gttctcatgc
     5701 gccgtgataa cagggcgata caaccggcga ggtcttcggc cgaaacggta ctggcatgaa
     5761 gctgcggagc cagcccgaga gcggcggcat accgcatgcc ggtccgaaca cccatctttc
     5821 gcgccacggg gttcatgtaa aggatttccg cctgcggggc atcccgtgtg ataacggcaa
     5881 caggcttgcc cgaccactct gaatgcctga ggcacaggat ctggagaggc aaatccgcaa
     5941 tccatacgca cgcaagccgg gacatcgcac aagaccttat cctgcgtcag gatgctttac
     6001 acagtgcaat tggagcggca aatctgttat cgctacgcat gccaacggga acaccacgat
     6061 attccctgtg aacccatccc ggcgcatgct gtttatcctt gatcgcttca agactgatac
     6121 cggccgttgc gtcgaatttt cccacgcgcc gcgcctgggt gcggagagag ataagcgagc
     6181 cgagggacga agcatgcacg ggtttgcgcg tcaggcatac ggttgccacg tttttgacat
     6241 gagaaagccg gacaatgcgg gaaagaagcg gcataggaat gcataccggc gatgcatcgc
     6301 tgagatcaat caccacaagg ccgaacccgc cggagcggat cagcatatcg gcggcgcgag
     6361 gggtatcgga tagttgagag aggcgcacga cgataagagc ggcaaggtca accccgtacg
     6421 cagcggcatc cagcgggaag aagatatccg atgccttgcc cacccatgcc gtcggctcac
     6481 cggcacactg ggcctggcgc acaagcatca tgacactgga aagcgccgcc gggttttccc
     6541 atgcggagat ttccgtcagt cgccctcgca attctttgta ccgccactcg gacctgtcgg
     6601 cgtgtatgtg gagcgcagaa gccgatttaa tgggaatttc cctgagcata tcctgcactg
     6661 ctgagatcac cacacaccta ccgggacagg tttgaagcac acataatata cacgtaataa
     6721 tacgggtatt acaggaaatg tcaagggcag gggagtgccg ggagctcggt cccttcgtac
     6781 cacacaaaaa gccgcacctg agccatcttc aggcgcggct tttgtcgttt tgggaggcat
     6841 ttaattcatg aattgcctcc tacctgcaaa taacctaccg agccagcacc atcctcctcg
     6901 tcaccacgcc ctgcggtgcg gtgagccggt agacgtacac cccgctggca accgcgcgac
     6961 ccatagcgtc ct
//
LOCUS       DAOTNG010000163         6961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9435_length_6961_cov_5.019983, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000163 DAOTNG010000000
VERSION     DAOTNG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_6961_cov_5.019983"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="P5179_08785"
     CDS             <1..1713
                     /locus_tag="P5179_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS95350.1"
                     /translation="GDGFLDICQGGLVYHNLGPEGAFSFAPPESLGSVPRMNALVDWN
                     GDGLIDIVRSESTGGGDPSPSIWSKDSSGSPYTKEGVWRGYTGHGRIILHRNTGSASH
                     PRFSAMPETLRAGGEWLDVPGGGMPAFGDMDGDGDDDLLVGTTHDLFYFENSGTRVAP
                     VYRRGAPLDIGLEEIYVRPVVADMNGDGMPDVLIAQENGDIKVVENLGRHDHEDRPRF
                     GVPSRIQQRDPLIDAGSVGAFDVADVNEDGRPDIIASNSYGEVWQWFSASPDCSWVFG
                     AGTRVRAGGKLIRIFAGASGSIQGPGEARYGYGAPEVADWNGDGALDLILADVWGKHT
                     LYCGLGADLRRTDANAGLELSEGQPLRFANPRDVVKPAWNWWNPVGGELVTQWRTRGE
                     VLDWNGDGEVDYVTIDHEGYLALAPGVRTREGRMLEPLERVFLNEAGEPLLLTPGTCG
                     RSGRYKIALADWDGDGDMDLVRGVATPSGNTMHTPSEETGCAFLFENIGNHRFAFRGE
                     MVPDPTIQLAGHSSCPQPFDFDGDGKLDLLIGAEDGHIYAFHRSYLEKDLPRVQVTRV
                     RITH"
     gene            complement(1749..4214)
                     /locus_tag="P5179_08790"
     CDS             complement(1749..4214)
                     /locus_tag="P5179_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS95351.1"
                     /translation="MDVDRFLKRIGAESGWRRQVVHVQRIPAREATYREPDVPLDAAI
                     ANVLKAQGISRLYCHQADALESARSGRGFVVVTGTASGKTLCYDLPIFERLLVEPDSC
                     ALCIYPTKALAQDQLRGVLRYLQGLGLGVKAGTYDGDTAPTTRRVLRNEGQVILTNPD
                     MLHAGILPHHARWARFFEKLRYVVLDEIHTYRGVFGSNVAGVLRRLNRICAHYGSKPL
                     YLCSSATIANPLEHAENLTQRSLTLIDRDGSPRGEKSFVLWNPPLLDDPSAGETPERR
                     SALSDAQYCMVSLIEERTPVIAFVRTRVSAELLYRFVQDVLSHRNASLARRVRAYRGG
                     YLPEERREIERQLFEGELLGVISTNALELGIDIGTLEASILIGYPGTIASTWQQAGRA
                     GRGKERSLVVLVAHNTPIDQYLMNHPQYFFERNPEHAIIDPTNPHIAIGHLLCAAQEL
                     PIAPEERVDLFGEYTPALVEILADEGHVRRVGERWHFARSGYAAAKIGLRNMDDTTYT
                     IMNETDQGPQAIGTLDEISALSQVHTHAVYLHDAETFFVDKLDVDRKIAHVRREGVDY
                     YTQSVSESSIVVRNTEREGAWRVSKLALGDVTVSLNVVMFKKIKFGSRESIGYENLDL
                     PTQTLDTTAFWLVPRAAALDECRAWGRVPAEGLKGIANVLVEIVPLFVMADTTDIGSI
                     VDSSNLGTPAIFLYDKYPGGIGFSERTYQWTEQIFTAAWELVSECPCIDGCPSCVGAP
                     PPAGGHDAAGERDIIPDKETALILLHHFLEKPPYTPKQRVVPSNTRVLPAVEEPNVPV
                     KPLPANEEAKVRSRLQWLKRKKA"
     gene            4403..5137
                     /locus_tag="P5179_08795"
     CDS             4403..5137
                     /locus_tag="P5179_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS95352.1"
                     /translation="MKGVILAGGTGSRLFPLTKVTNKHLLPVYDRPMIYHPIQALVNA
                     GIRDIMIVTGGENAGDFLRLLGNGSEFGLQHLNYAYQEKPGGIAQALALAEHFVGNEK
                     FVVALGDNIIQKSIRAAAQAFEHQPEGAKILLAEVDNPRSYGIARIDGNAVVEIIEKP
                     SDPPSNFAVIGIYFYTPEVFKIIKTLKPSGRGELEITDVNNEYLRRGTLSFEYLDGWW
                     ADCGESIPWLLRANNLVARDGANNAG"
     gene            5279..6358
                     /locus_tag="P5179_08800"
     CDS             5279..6358
                     /locus_tag="P5179_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95353.1"
                     /translation="MADKIRMGFIGCGFMGQLAHIANYARLHDECDLVAVADVRPLLA
                     KKVAARYGIRETYTDHRELLEKADVDAVAAIMYYGHHYAVVPDVIESGRHLITEKPMA
                     CSASSARKWKAAADAKLLVYMVGYMKRWDLGARWVSDMVREWKTTNEFGEFTHLRCLM
                     SGTDWTWNPYPVISSDEQPVASLPPHEPFPARFTKRQADYFNMNINFYVHQVNLMRFL
                     LHEQITLDYCHPAGKVLIGSTESGKAVSLEMGISSIPQTWDEVYQIAFEKADIELRMP
                     APLRGQHNGEVVVRRTVNGKTYETLRPNFPVPSWSFLEQAKGFLEAVRTGRVPFDSTG
                     DAVRDLEFFETQADMMQGEGGVARK"
     gene            6391..>6961
                     /locus_tag="P5179_08805"
     CDS             6391..>6961
                     /locus_tag="P5179_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95354.1"
                     /translation="MVVELEKGELRATVVDNAAHLERAGHREGYNGLAALTSDAQQAS
                     PIVPFYAGMNLELYFDGVVTDRSTLFEPRRAPMAVEKIDQATARLHQPPVPYWKVESW
                     TEFTLREPNYIDFRYRAIPHERTFEQNWLGVFWATYVAYPEDPGIWFPTATPEGAKTM
                     RHFSAVHGDESTHRHVADSEEITVSHDHRI"
BASE COUNT         1485 a         1922 c         2139 g         1415 t
ORIGIN      
        1 ggggacggct ttctcgacat ctgccagggt gggttggtgt accacaatct cgggcccgag
       61 ggcgcttttt ccttcgcgcc tccggaatcc cttggaagcg tgccccgcat gaatgcgttg
      121 gtcgactgga atggcgatgg cctgatcgat atcgttcgaa gcgagagtac cggcggcggg
      181 gatccgtcgc cctccatctg gagcaaagat tcgtccgggt cgccctatac caaagagggt
      241 gtgtggcgtg gttataccgg gcacggacgg atcattctcc acaggaacac cggaagtgcc
      301 tcccatccga ggttttccgc gatgccggaa acgttacgcg cgggagggga gtggttggat
      361 gttccgggag gtggtatgcc tgccttcggt gatatggacg gagacgggga cgatgatctg
      421 cttgtcggga ctactcacga tttgttctac ttcgaaaact caggcactcg agtcgccccg
      481 gtgtatcgtc gcggcgcccc tctggacatc gggctggaag agatttacgt gaggccggtg
      541 gttgccgaca tgaacgggga cggaatgccg gacgtgctga ttgcgcagga aaacggcgac
      601 atcaaagtgg tcgaaaacct tgggcggcac gatcatgaag acaggccacg attcggcgta
      661 ccctctcgaa ttcaacagcg ggacccactg atagacgcgg ggagcgtggg agcctttgat
      721 gttgccgacg tgaacgaaga tggacgaccg gacataattg cttcaaactc gtacggagaa
      781 gtatggcaat ggttctcggc atctcctgat tgctcctggg tgttcggtgc gggaacacgc
      841 gtgcgggctg gagggaagct catacggata ttcgccggag cctcgggaag catacagggg
      901 ccgggagagg cacggtacgg atacggggct ccggaagtgg cggattggaa cggggacggc
      961 gcgctggatc tcatacttgc tgacgtgtgg gggaaacaca cactttactg cggactgggc
     1021 gctgatttgc gacggacgga cgcaaatgcg ggattagagc tctcagaggg acagccgctg
     1081 cgtttcgcga acccgcgaga cgtcgtaaaa ccggcgtgga attggtggaa tcccgtcggt
     1141 ggagagcttg tgacgcagtg gcgaacacgc ggtgaggtgc tcgattggaa cggcgatggc
     1201 gaggtggatt acgtcacgat cgatcacgaa gggtacctcg ctcttgctcc cggtgttcgc
     1261 acgcgggaag gcagaatgct cgaacccctt gagcgagtgt ttctgaatga agccggggag
     1321 ccgcttctgc tcacaccggg aacctgtggc cgcagcggcc gttacaaaat cgcgctggcc
     1381 gattgggatg gcgacgggga catggatctc gtaaggggtg ttgcaacccc ctccggcaat
     1441 accatgcaca caccttccga ggaaactggt tgcgcgtttc tgttcgagaa cattgggaac
     1501 caccgctttg cgttcagggg ggagatggtt cccgacccga ccatccagct tgcgggtcac
     1561 agctcgtgcc ctcaaccgtt cgactttgac ggcgacggca agctggacct tctaatagga
     1621 gctgaagatg ggcacattta cgcgttccac cgctcctatc tcgaaaagga tctgccgcgg
     1681 gttcaggtaa cgcgggtcag gataacgcac tgagagactg gcacaccgca actccgcaca
     1741 ggtgtgaatc aggctttttt ccgtttgagc cactgaaggc gcgaacggac ttttgcctcc
     1801 tcgtttgcgg gtaatggttt cacgggcacg ttcggttcct cgaccgcggg aagcacgcga
     1861 gtgtttgatg gcaccacgcg ttgcttgggt gtgtaaggcg gcttttccaa aaaatggtgc
     1921 aacaggatca gcgccgtctc cttgtccgga atgatgtcac gctcaccggc tgcatcgtgg
     1981 ccgcccgcag ggggcggagc tcccacgcag gacggacagc cgtcgatgca ggggcactcg
     2041 gaaaccagtt cccacgcggc cgtaaagatc tgctccgtcc actggtaggt cctttcggaa
     2101 aaaccgattc cgcccgggta tttgtcatac aagaaaatcg cgggcgtgcc gaggttggag
     2161 ctgtcaacaa tgcttccgat atcagtggtg tcggccatga caaagagcgg gacaatctcc
     2221 acgagaacgt tggcaattcc tttcaagcct tcagcgggaa ctctgcccca ggccctgcat
     2281 tcatcgagag ccgcagcacg gggaacaagc cagaatgcag tcgtgtcaag cgtctgcgtg
     2341 ggcaggtcaa ggttctcgta accgattgac tccctgcttc caaacttgat tttcttgaac
     2401 atcactacgt tgagacttac ggtgacatcg ccgagcgcca atttgctcac acgccaggca
     2461 ccctcccgtt cggtgttccg tacgacaatg ctcgactcgc tgacagattg cgtgtagtaa
     2521 tccacgcctt ctcgccgaac gtgcgcgatt ttgcgatcca cgtcgagttt gtcaacaaag
     2581 aaggtctcgg catcgtggag gtagacggcg tgagtgtgta cctgggaaag cgcgcttatc
     2641 tcgtcgagtg tgccgattgc ctgaggcccc tgatccgttt cgttcatgat cgtgtacgtc
     2701 gtgtcgtcca tgttgcgaag accgattttc gcggcggcgt accctgatct cgcgaagtgc
     2761 cagcgctcac cgacccgccg aacatgtccc tcgtctgcaa gtatttccac caacgcaggc
     2821 gtgtactcac cgaacagatc aacacgctct tcgggggcga tggggagttc ctgtgccgca
     2881 cagagcagat gaccgatcgc tatgtgcgga ttggtgggat cgatgatggc gtgttccgga
     2941 tttctttcga agaaatactg cggatgattc atgagatact ggtctatggg cgtattgtgt
     3001 gcgaccagca ccaccaggga ccgttctttg ccccgtcccg cccgaccagc ctgttgccac
     3061 gtgctggcaa tcgtgcccgg gtaaccgatc agaatgctgg cctctagcgt gcctatgtct
     3121 atgccgagtt cgagggcgtt ggtgctgatc acgccgagaa gttcaccctc gaagagctgc
     3181 cgttcgattt ccctacgttc ttccggtaga tatcccccgc gataggctct cacccggcgc
     3241 gcgagtgacg cgttgcggtg agaaaggaca tcctggacga aacggtacag aagctctgcg
     3301 gagacgcgtg ttctgacgaa agcgatgacg ggagtccgct cttcgatcaa ggacaccatg
     3361 caatactgcg cgtcggagag cgcgctcctg cgttccggag tctctccggc ggacggatca
     3421 tccagcagag gggggttcca gagcacaaag ctcttctcgc cgcgaggtga tccatctcgg
     3481 tcgataagcg tcagacttcg ctgggtgagg ttttcggcat gctccagtgg gttggcgatg
     3541 gttgcggatg agcaaagata cagcggtttc gacccgtagt gcgcgcagat gcggttcaag
     3601 cgtcgaagca ctcccgcgac gtttgaaccg aacacgcccc ggtacgtgtg gatttcgtcc
     3661 agaacgacgt atctgagctt ctcgaaaaaa cgcgcccaac gcgcgtgatg aggcagtata
     3721 ccggcgtgca gcatatcggg gttggtgaga atcacctgcc cctcgttgcg cagaacccgg
     3781 cgtgtcgtag gtgccgtatc accgtcgtac gtccccgcct tcacccccaa tccgagcccc
     3841 tggaggtaac gcaatacacc gcggagttgg tcctgggcca atgctttcgt cgggtagatg
     3901 caaagcgcgc aggagtccgg ctccacaagg aggcgctcga aaatcggaag atcgtaacaa
     3961 agcgtttttc cgcttgcggt tccggtgaca accacgaaac cacgcccgct tctcgccgac
     4021 tccagcgcat cagcctgatg gcagtacagg cggctgattc cctgggcttt caacacgttg
     4081 gcaatggcgg catcaagggg tacgtcgggt tcccggtacg tcgcttcgcg agccggtatc
     4141 cgttgcacat gcacaacttg gcgacgccat ccagattcgg ctccaatacg tttcaaaaac
     4201 ctgtctacat ccatgggatt accctttgtt cgtatgcaaa gagtccgacg ggggctttgc
     4261 ggactctgtt ttcctgcgtt aacgtacgca aggaaattgc tggaacccac agcccgtgcg
     4321 cgttcgcttt gccgatgtgg ggaaccgaca ccggacgagc agaagccaag gtaatataca
     4381 gatctgggaa gggagcgcgg ccgtgaaagg cgtcattctg gcagggggaa cgggttcccg
     4441 gttgttcccc ctcaccaagg ttaccaacaa gcatctcttg cccgtatacg accgccccat
     4501 gatctaccac ccgatccagg cgctggtgaa cgcggggatc cgggacataa tgattgtgac
     4561 gggaggcgag aacgccggtg atttcctgcg ccttctcggg aatggcagcg aattcggttt
     4621 gcagcatttg aactacgcgt accaggagaa accgggcgga atcgcccaag cgctcgctct
     4681 ggcggagcat tttgtaggga acgagaagtt cgtggtagcc ctcggagaca acatcatcca
     4741 gaagagcatt cgcgcggcgg cacaggcatt tgagcaccag ccggaagggg cgaagatcct
     4801 cctggccgaa gttgacaacc cgcgcagtta cggaatcgca cgtattgacg gtaacgccgt
     4861 agtcgagatc atcgaaaaac cgtcggatcc ccccagcaat ttcgcggtga taggcatcta
     4921 cttctacact ccagaggtgt tcaaaattat aaagacgtta aagccgtctg gccgagggga
     4981 actcgagatc acagacgtga acaacgaata cctgcggcgc ggtacgttgt cgtttgaata
     5041 ccttgacgga tggtgggccg actgcggcga atcaatcccg tggctccttc gtgccaacaa
     5101 cctcgtagcg cgcgacggtg cgaacaacgc gggataggct gatccacccg gcgccgaagc
     5161 gttcgtgcac cgcacaggcc ggacgtccag cccgcgcacc attttctttg aatgatggat
     5221 gaattgtctt cgcatttccc gggaccagca agaacacatc gcgcggggag gcacacgcat
     5281 ggctgacaaa atccggatgg ggttcatcgg atgcggtttc atgggtcaac tggcccacat
     5341 cgccaactac gcccgtctgc atgacgaatg tgatctggtc gctgtcgcgg acgttcgccc
     5401 tctgctggcg aaaaaggttg ccgcgcgtta cggaatacgc gaaacgtaca ccgaccatcg
     5461 cgagctgctg gaaaaagcgg atgtcgacgc cgtggcagcg attatgtatt acgggcatca
     5521 ttacgcggtc gtccccgacg ttatcgaatc gggaaggcat ctgatcactg agaagcccat
     5581 ggcgtgcagt gcctcgagcg cgcggaaatg gaaggccgcg gcggacgcga agctcctggt
     5641 gtacatggtc ggatacatga aacgctggga tctcggtgct cgatgggtgt ccgacatggt
     5701 tcgggagtgg aaaacgacca acgagttcgg cgagttcact catcttcgat gcctgatgag
     5761 cggaaccgat tggacctgga atccctatcc cgtcatctct tcagacgaac aaccagtggc
     5821 ctcattgccg ccgcacgagc cattcccggc ccgtttcacg aaacggcagg ccgattactt
     5881 caacatgaac atcaatttct acgtgcacca ggtgaatctg atgcgttttc ttctccacga
     5941 gcagatcacg cttgattatt gccatccagc tgggaaggta ctcatcggtt ccacggaaag
     6001 cgggaaagca gtgtcactgg aaatggggat ttccagcatt ccccagacgt gggacgaagt
     6061 ctatcagatc gcgtttgaga aagcagacat agaactgcga atgccggccc cgttgcgcgg
     6121 gcaacacaac ggcgaagtag tggtgcggcg gacggtgaac ggaaagacgt atgagacgct
     6181 cagacccaat ttccctgttc cgagctggag tttcctcgaa caggcaaagg gattcctgga
     6241 ggcagttcga accggcaggg ttccgttcga ttccacgggt gacgcagtcc gcgatttgga
     6301 gtttttcgag acccaggcgg acatgatgca gggagaaggc ggagtcgccc gaaagtgaat
     6361 gcgtgaacca acagaggagg gggcgggaat atggttgtcg agctggaaaa aggagaactg
     6421 cgggccacgg ttgtcgacaa cgcggcgcat ctggaacggg ctgggcaccg ggagggctac
     6481 aacggcctgg cggcactcac cagcgacgcg cagcaggcga gccctatcgt ccctttctac
     6541 gctggaatga atctcgagct ctatttcgac ggtgtggtga ccgaccggtc aacacttttt
     6601 gaaccgcggc gtgctccgat ggcggtggag aaaatcgatc aggcgaccgc gcggctccac
     6661 cagccacctg ttccctactg gaaagtggag agctggaccg aattcacact acgggaaccg
     6721 aattacatcg acttccgtta tcgcgcaatc cctcacgagc ggacgtttga gcagaactgg
     6781 ctgggcgtgt tctgggcaac ttacgtcgcg tacccggagg atccagggat ctggtttcct
     6841 actgcaaccc cggaaggcgc gaagaccatg cggcatttct ccgcagttca cggtgacgaa
     6901 agcacgcacc ggcatgtcgc ggacagcgaa gagatcacgg tgtcacacga tcatcggatt
     6961 t
//
LOCUS       DAOTNG010000164         6931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9496_length_6931_cov_5.181065, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000164 DAOTNG010000000
VERSION     DAOTNG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9496_length_6931_cov_5.181065"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="P5179_08810"
     CDS             complement(<1..1325)
                     /locus_tag="P5179_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS95355.1"
                     /translation="MPGIPSEFFVAPHGNDSNEGSACAPFATLERARDQIRASSASAG
                     LPEGGVTVWIRGGVYQRQMAFELTEEDSGKESSPITYRAWPGESARIIGGVMISHFSR
                     VDDASVRKRLQPSVRDRVLSADLSEYGVTDCGTLTSRGFARPVLPAHAELFIEGEPMT
                     LAQWPQSGEFLKIAGFGKPLTDEWGTTTGDLTGGFTYQGDRPLAWEPDDDIWVHGYWS
                     YDWANSYERVRHIDPHSRTVTTHPPHGNYSYRVGQRFYFLNVLEELDAPGEYYVDRRR
                     GRLYLLPPDEQEVPSDVILSVLEAPLVAMQRVSHVSFDGLTLECSRGDGLVATDCEHV
                     SVRSCTIKNLGNRGIVIRGGRNVAVAGCTVFNNGDGGLDVEGGDRQTLEPADHVVVNN
                     HIHHIARWSRCYQTAVNVHGVGHLIAHNLIHDLPHCAILFWGNEITIEN"
     gene            complement(1593..2024)
                     /locus_tag="P5179_08815"
     CDS             complement(1593..2024)
                     /locus_tag="P5179_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95356.1"
                     /translation="MAGSEILKRHWRDFDNLRRAFRNHEIVIGLDRRFVLFWVLLSGA
                     ECRFAARATGWMLVVSILVAVAGASLAAWHALYILAALCALCAVFLWRFLVEFSVKTA
                     RNAVLNDERLFRKWFSELRLSVFVKSTGEYVWNDAAQRARE"
     gene            complement(2011..2886)
                     /locus_tag="P5179_08820"
     CDS             complement(2011..2886)
                     /locus_tag="P5179_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95357.1"
                     /translation="MSPPAPIFSDRITAIAAELANLGKRPISAATFQEAKRLHQQLDA
                     LLREFVPCDRCGNFFPNIAQLTLDGRSANMCASCAITALQRGTIDTPVTEQARITHDE
                     PKNSPEKSTPVLQDAASVAPEMVSPARPPVPEKASSTETPRRALPVREPRARVEEDED
                     EPEVRLERSDEPQDGEDEEEKDDSPAVVSARPRVSRAPRLVARGAPNGADQMKASHGE
                     VARYLNCPTRDVRQIAKLIEEISPPMDIPKTTRYVLAELRNARSKIPPDTVSKVVHFL
                     KDGFPAGGDGAHGGQ"
     gene            complement(3161..4093)
                     /locus_tag="P5179_08825"
     CDS             complement(3161..4093)
                     /locus_tag="P5179_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRS95358.1"
                     /translation="MRTKTRAGTNRMAPDRTTQNRDSVRSDLGTALENLCNTYDESAL
                     DPDPLVVVREFSDPRDQEIAAFFSAMFAYGKARIIVRNLRDLFRRMPGGPYAFVTASH
                     HSNDAGCLAGWRHRLHTGEDVAIMSAILGQVLRKHGSLERVFCRGLPGDAPDVGVALD
                     AFVSFLREQGGRRIEQRRFFRHLLPSPADGSACKRMNLFLRWVVRRESPDLGLWGTVP
                     PSLLVLPLDTHVARICRQIGLTRRSTIDWKMALEVTRKLRRFDPADPIRYDYALSRLG
                     ILGDCPLKMDISNCSRCSLQTYCVTFSERGSSQS"
     gene            complement(4151..>6931)
                     /locus_tag="P5179_08830"
     CDS             complement(4151..>6931)
                     /locus_tag="P5179_08830"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HRS95359.1"
                     /translation="STAACMYQITPSTVVFPESEAEVVAVVRRAAETGIPVTARGGGS
                     SLVGQALGRGVILDFSRFMRAVLELDENRRRVRVQPGITLGVLNRHLAARGLMFPPDP
                     SSGDYATIGGMIACNSSGARSVKYGSTIDYVAAVRCVLADGSVVEFRGSEAVPISPQT
                     SLLTCLVEEIPAILRQHDSRIREMFPKCAKNSSGYQLAKALRPDGVDLASLIVGSEGT
                     LGIVTEAELEIVPKPLASSMVLVNFASVEAAGEAVHTILPLSPSAVEIMDQTALDMIR
                     AHRPELAAFLPTDAVAQLFVEFDGDNSETTESACRMAVERSHDLATGHVFAFDAATHS
                     ALMNVRKATLPILYTLPGKKRPVAFVEDVIVPPESLGTFIGDLKAIFQGFDVEAAIYG
                     HAGQGNLHVRPLLDLTDARDIAKLEPLASAVFGAVCRFGGSMSGEHGDGRARAPFLRQ
                     MYGELSDVFVDVKELFDPSNILNPDVKVTRAPHALDSDLRLGSGHTLQPLDTPLPVAP
                     DEITDIISRCHGCGTCRIPEPPVTMCPVYKALGDEAATPRAKANLLRLFASGKLPESF
                     RKTKDWKRILDTCIGCRMCSFECPSGVDIGRIMLIAKGQYCEHKGRSIAEIAMGASDF
                     LSSAGRFFSPLSNVVARQPLFGAALDLFSGIDHRRRLPRFAGKTFSSWHRTHGKNEQS
                     AGAEAVVYFPDQWAQYHDPDVGKALHAVLNRLGIRMIVPETLPCGIAAAANGGLTRVR
                     RLASHNVDALRPYAEAGLRIIATEPTATLFLKDEYRDILGSSAAELVAGCMQDASQFI
                     EQRLRTGTDLPLRALDFSVAYHAPCHLKAMRVGLPAVDILRRIPSLRVQILDEGCCGM
                     AGTYGMKRAHFETSMAIGKRLLDHLADPSINYGVTDCSTCKLQMEFGTRKTTLHPLKL
                     LACSMGAEVPLR"
BASE COUNT         1410 a         2079 c         2038 g         1404 t
ORIGIN      
        1 ttgttttcta tggtaatctc atttccccag aaaaggatcg cgcaatgggg caggtcatga
       61 atcagattgt gtgcgatcag gtgcccgaca ccgtgaacgt ttactgccgt ctgatagcaa
      121 cgggaccacc gtgcgatatg gtggatgtgg ttgttgacca ccacgtggtc tgccggttca
      181 agagtctgcc gatcgcctcc ttcgacgtca aggccgccgt cgccgttatt gaacaccgtg
      241 catccggcta ccgccacgtt ccttccaccc cgtatcacaa tcccccggtt tccgaggttc
      301 ttgatggtgc atgatcttac tgatacgtgc tcacagtccg tggcgaccaa gccgtctccc
      361 cgcgagcatt caagcgtgag accgtcgaac gagacgtgag agaccctctg catcgccacg
      421 agaggagctt cgagaacaga aagaatcaca tcgcttggta cctcttgctc atccggggga
      481 agcagatata gtctccccct gcgccggtca acgtaatact cacccggagc gtccagttcc
      541 tcgaggacat tcaggaaata gaacctttgg ccaacccggt agctgtagtt tccgtgcggg
      601 gggtgggtgg tgacggttct cgaatgcggg tcgatgtggc gcacacgctc gtagctgttt
      661 gcccaatcgt acgaccagta gccgtggacc cagatgtcgt catccggttc ccacgccaac
      721 ggacgatctc cttggtacgt gaacccgcca gtcaggtcgc cagttgtggt tccccattca
      781 tctgtcagag gtttgccgaa ccctgcgatt ttcagaaatt cgcccgattg gggccattga
      841 gcgagcgtca tgggctcgcc ttcgatgaat aactcggcgt gggcaggcaa cacgggccgg
      901 gcaaatccac gcgaagtcag cgtgccacag tcggtaacac cgtattcgct cagatccgcc
      961 gacagaacgc gatctcgcac ggacggttgc agacgtttgc gcacggatgc atcatccaca
     1021 cgcgaaaagt gggaaatcat caccccacca atgatgcggg cactctctcc gggccaggca
     1081 cggtacgtaa tcggggatga ttctttcccc gagtcttctt ctgtgagttc gaaagccatt
     1141 tgccgctgat acacgccgcc gcgaatccac accgttacac cgccttccgg caatcccgcg
     1201 gatgcggacg aagcccttat ctggtcccgg gcgcgctcca gtgtggcaaa gggtgcgcaa
     1261 gccgagccct cattgctgtc gttgccatgc ggggcgacga aaaactcgga gggtatacct
     1321 ggcatctaag ttccttcggt atcagtttgc ctgtttcggg tgtctttgcg cgaggcacgc
     1381 aagaatatct tggccgctgg attgcgcctc ggctactcct acgtcgcaga aactggacga
     1441 tgctgacaag aggaagtgtg ttctgaaacg ggtccctgcg atcttcgaac tgctatagtt
     1501 gcgcccatgc gcgcgcccgc caaaccgacg gccgccactc tcttcccccc gtttctccga
     1561 ctttcccctg tctcttcaag cggtgggggc agctactccc gcgcacgttg tgcggcatcg
     1621 ttccatacgt attcgccggt agatttcacg aaaacgctca gccgcaactc cgaaaaccat
     1681 ttccgaaaca gacgctcgtc gttcagaacg gcgtttctgg cggttttcac ggaaaactcc
     1741 accaggaaac gccaaagaaa caccgcgcaa agagcacaga gagccgcgag aatgtacagg
     1801 gcatgccaag ccgccagact ggctcccgcg acggcgacaa gaatcgacac aaccagcatc
     1861 caacccgtcg cgcgcgcggc gaaccgacat tctgcacccg aaagaaggac ccagaacaaa
     1921 acaaacctcc ggtccagccc gattacgatc tcatggttgc ggaaagccct tcggaggttg
     1981 tcgaaatcac gccagtggcg cttgagtatc tcactgcccg ccatgtgctc cgtcgccccc
     2041 ggccggaaac ccatctttca ggaaatggac cactttggaa acggtgtcgg gagggatctt
     2101 cgagcgcgca ttgcggagct cggctagtac ataccgcgtg gttttcggga tgtccatcgg
     2161 aggcgagatt tcttcaatca gcttggcgat ctggcgcacg tcgcgagttg ggcagttcag
     2221 gtatcgcgca acttccccgt gtgacgcttt catctggtcc gcgccgttcg gagcgccccg
     2281 ggccaccaga cgcggggcgc gcgaaactcg agggcgcgcg gagacaacgg cgggggagtc
     2341 gtccttctct tcttcgtcct caccatcttg cggttcgtcg gaacgttcca gccgcacctc
     2401 aggctcatct tcatcttctt caacacgagc gcgcggctcg cgcacaggca acgcgcgacg
     2461 tggagtctcg gttgaacttg ctttctcggg aaccggcggc cgcgcaggag aaaccatttc
     2521 tggcgctact gaagccgcat cctgcagaac gggtgtgctt ttctcagggc tattcttcgg
     2581 ctcatcgtgc gtgattctcg cttgttcggt caccggcgtg tcgatcgtcc cacgctggag
     2641 tgcggtgatc gcacaggatg cacacatgtt cgccgagcgg ccgtccaacg taagttgtgc
     2701 aatattggga aaaaagttgc cgcaccgatc gcacggcaca aattcccgaa gcagggcgtc
     2761 aagttgctgg tgcagccgct ttgcttcctg aaaagtcgcg gcggaaatgg ggcgtttccc
     2821 cagatttgcc agttcggcgg ctatagcggt gatgcgatcg ctgaaaatgg gcgcgggagg
     2881 ggacattcag ctctccggaa accgttacga gcatgggcac gatgggtgtg tacgtcggaa
     2941 gatagcaggg gagccgaacc gcggcaagcc gggactcact ccgtactgcc ggttgcagcg
     3001 gcaaatggta cagtccttcg ggccacatgc ttcagcgagt gccacgggcg aacagaactc
     3061 gtgaatccca cagccgggga tcctgaacaa ctggatacac acccacgaaa atgcccgatt
     3121 accattgtcc tcgaacgccg ggtccatgtc ctgaagcggt tcagctttga gacgaacctc
     3181 gttcgctgaa cgtaacgcag taggtctgca gggaacagcg cgagcagttg ctgatgtcca
     3241 tcttcaacgg gcaatcgcca agaatcccga ggcgactcag agcgtaatca taccggatcg
     3301 gatccgccgg gtcgaagcgc cgaagctttc gcgtaacctc caaagccatc ttccagtcga
     3361 tcgtggatcg gcgggtgagg cctatctgcc tgcagatccg ggcaacgtgt gtgtcgaggg
     3421 gcaacacgag caacgacggt ggaacggttc cccagagacc caggtcggga gattcgcgtc
     3481 ggactaccca cctcaaaaag aggttcattc gtttgcatgc ggatccgtcc gcgggtgacg
     3541 gcagcagatg cctgaagaag cgtcgctgct cgatccttcg ccctccctgt tcccgcagaa
     3601 acgacacaaa tgcgtccagc gcaacaccga catccggcgc gtctccaggc aatccgcggc
     3661 agaacacgcg ttccaacgag ccatgtttcc tcaggacttg cccgaggatc gcggacatga
     3721 tggccacgtc ctctcctgtg tggagacggt gtcgccatcc ggcaagacat cccgcgtcgt
     3781 tcgagtggtg tgaggcagtg acgaacgcat agggtccgcc gggcattcgg cggaaaagat
     3841 cacgcagatt ccgcacgatg atcctggctt tgccgtatgc gaacatcgcg gagaagaacg
     3901 ctgcaatctc ctgatcccgc gggtcagaga attcgcggac tacgacgagc gggtccggat
     3961 ccagtgcgga ttcgtcgtaa gtgttgcaca ggttctccag ggcggtaccg aggtccgatc
     4021 gcactgaatc cctgttttga gtggtgcggt ccggcgccat ccggttcgtg ccggcgcgcg
     4081 tttttgtcct cacggcgtgt gccgcggcca caggtcggtg tacatcgttg cgacggcgca
     4141 aggggcgttc tcacctgaga gggacttcgg cacccatgga acacgcgagg agcttcaggg
     4201 ggtgcaacgt cgttttcctc gttccgaact ccatctggag tttgcatgtg ctgcagtctg
     4261 tcactccgta gttgatcgag ggatcagcca gatggtcaag cagcctcttg ccgattgcca
     4321 ttgaggtttc gaaatgcgcg cgtttcatgc cgtatgttcc ggccataccg caacagcctt
     4381 catcgagaat ctgtacccgg agcgacggga tgcgcctgag gatgtctact gccggcagcc
     4441 caacccgcat cgccttcagg tgacagggtg catggtatgc gacactgaaa tcgagtgccc
     4501 gtagaggaag gtcagtgcca gtgcgcagtc tttgttcgat gaattgagag gcgtcctgca
     4561 tacagccggc gaccaactcg gcagcgctgg agccgagaat gtcacggtac tcgtccttga
     4621 gaaagagcgt ggccgtaggt tccgtggcaa tgatgcgcag gcccgcctcg gcatacgggc
     4681 gcagtgcgtc aacgttgtgc gacgcgagtc ggcgtaccct cgtgagcccg ccgttggcgg
     4741 cggcagcaat tccgcacggg agagtctccg gcacgatcat acggattccg agcctgttca
     4801 acaccgcgtg caacgctttt cctacatcgg ggtcatggta ttgcgcccac tggtccggga
     4861 agtacacaac ggcctctgcc cccgcagatt gctcattctt tccgtgtgtg cggtgccacg
     4921 acgagaaggt ctttccagcg aaccggggca gtctccgccg gtggtcaatc cctgagaaca
     4981 ggtccagtgc ggctccgaac aacggttgcc gtgcaaccac gttggaaagg ggtgagaaaa
     5041 acctgcctgc gctactgaga aagtcggatg ctcccattgc gatttcagcg atggaacggc
     5101 ctttgtgttc gcagtactgg ccctttgcaa tcaacatgat gcgcccgatg tctacgccgg
     5161 aagggcattc gaacgagcac atgcggcagc cgatacacgt gtccagtatc cgtttccagt
     5221 ctttggtctt gcgaaaggat tccggcaact tgccacttgc aaaaagacgg agcaggttcg
     5281 cttttgcgcg cggagtggcg gcttcgtctc cgagtgcctt atacaccgga cacatggtga
     5341 caggcggttc gggtatgcgg cacgttccgc atccgtggca gcgcgatatt atgtccgtga
     5401 tctcatcagg tgctaccggg agaggcgtgt ccagaggttg aagagtgtgc ccgctgccga
     5461 gccgcaggtc gctgtccaac gcgtggggcg cacgggtcac ttttacgtcc gggttcagga
     5521 tgttcgacgg gtcgaacaac tctttgacgt ccacaaacac gtcgctgagt tcgccgtaca
     5581 tttggcgaag aaaaggagcc cgcgctctcc cgtcgccgtg ttccccgctc atggagccgc
     5641 caaaccgaca caccgccccg aatacggcac tcgcaagcgg ttccaatttg gcgatatcac
     5701 gcgcatcggt gaggtcgagc agcgggcgga catgcagatt gccctgacct gcatgtccat
     5761 atatcgccgc ttcgacatcg aagccctgga aaatggcttt caaatcgccg atgaatgttc
     5821 ccagtgactc cggcggcaca attacatcct ctacgaatgc cacgggccgc ttttttccgg
     5881 gcaaggtgta aaggataggc agggttgctt tccgcacgtt catgagtgca ctgtgagtcg
     5941 ctgcgtcgaa cgcgaacaca tgtcccgtgg cgagatcgtg agaccgttcc acagccattc
     6001 tgcacgcgga ttccgtggtc tccgaattgt cgccgtcgaa ctccacgaac aactgtgcaa
     6061 ctgcatcggt cggcaggaac gctgcaagtt cgggacgatg ggcgcgtatc atgtccagcg
     6121 cggtctggtc catgatttcc accgcactgg ggctcagcgg aaggatcgta tgtacagcct
     6181 ccccggcagc ctccacgctg gcgaaattga ccagcaccat tgaagatgca agcggcttag
     6241 gcacgatctc gagttcggcc tccgtgacga tgccgagtgt gccctcagaa ccaactatga
     6301 ggctcgcgag gtctacgccg tcaggtctca gtgctttggc caactggtag ccgctggagt
     6361 tcttcgcgca cttcggaaac atctcacgga tgcgggaatc gtgttgccgg aggatcgccg
     6421 gaatctcctc aaccagacag gtcagcagag atgtctgcgg cgatatcggc acggcttctg
     6481 aaccgcggaa ctccacaact gacccgtcgg cgagaacaca acgcaccgcg gcgacgtaat
     6541 cgatcgtgga cccatacttg acggaacgcg cgccgctcga attgcatgca atcatgcccc
     6601 caatcgttgc gtagtccccg cttgaggggt ctggtgggaa catcaatccg cgagccgcga
     6661 gatgcctgtt cagcactcca agtgtgatac cgggctggac cctcacgcgg cgccggttct
     6721 cgtccagttc cagaaccgcg cgcatgaagc gactgaaatc cagaatgacc cctcggccaa
     6781 gcgcctgtcc gacaagtgaa gacccgccac cgcgggcggt gacaggaatg cctgtctcgg
     6841 ctgcgcgacg cacgacggcc accacttccg cttcggattc cgggaacacc acggtggacg
     6901 gggtgatctg gtacatacag gcagcggtgc t
//
LOCUS       DAOTNG010000165         6910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9523_length_6910_cov_4.409482, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000165 DAOTNG010000000
VERSION     DAOTNG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9523_length_6910_cov_4.409482"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="P5179_08835"
     CDS             complement(<1..481)
                     /locus_tag="P5179_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS95360.1"
                     /translation="MRLGAPVFDEAGTPEAWAAAHGIAGYRAAYCPVDGTASDDVVNA
                     YAEAAKKFDIVIAETGAWSNTISPDTETRDAAIRKCQDQLALADRIGARCCVNIAGSR
                     GTQWDGPHPDNLTRHTFDLIVQTVREIIDAVKPVRSYYTLETMPWVFPDSPESYSELI
                     "
     gene            complement(484..1983)
                     /locus_tag="P5179_08840"
     CDS             complement(484..1983)
                     /locus_tag="P5179_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRS95361.1"
                     /translation="MRIAIGGIYHESNSFSARLTTLEDFRSSSLLTGGAIIDYWRGTN
                     SEVAGFLDGIEAIGGEAVPTLVAWGMPSGPVAARDLEELAGELARRIAQEQDIAGVLL
                     VCHGAMVCETRTDADAFWLHQVRKAVGRHVPIVATLDFHANVTPEMVAAVDAVVGYRT
                     YPHTDYRDRGKEAAEILAKTLRGETHPIAHCVQVPVVPNLLTQFTACAPMRCLMDAAD
                     DVRRAAGCISASVFGGFQWADVPHHGISVVTVSEKGDPDTVRAGEAIARKAWDLRHSF
                     RTDLVSVEDAVRQANEPGPTPVVLVDTGDNVGGGAPGDGTIVLKELLEQHSSGAVVLV
                     HDPETVQRAIETGVRGRARFRIGGKTDALHGEPVETEGDVRLLSSGLFVNVGPMREGL
                     REDMGRTAVIDARGVTIIATEKRYQMWNLEQLRSLGIEPTRCRTIVVKAAIAHRAAYS
                     PIAGRMVELDTPGITALDIRRFTYRHQRRPVFPLDDDFHPLIQDQTTGA"
     gene            complement(2061..3008)
                     /locus_tag="P5179_08845"
     CDS             complement(2061..3008)
                     /locus_tag="P5179_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRS95362.1"
                     /translation="MHAPEPITMITFERWPEPWMEELRAAAPGSTIFRPQSREGIDWA
                     SLDALNGGINKDILAKAARLRWFHSPAAGVEGYLFPEMVESPVVLTNSKGVYSIFIAE
                     LAMGHLLALTTQVHANTVRPDRLTWLRDTLRGFELYGATAVVIGLGNIGSEVADRAKA
                     FRMRVIGVDPVRKAAAGVELVRPDRIDDVLPLADVIFITAPATTARKLFDRERLQSLK
                     KGAYLINSSRGPVVDTDALVEALRSGHLGGAGLDVTDPEPLPADHPLWTLPNVTITPH
                     IGGMTDAVDRRRFVILRENLWRFVRGVPLLNVVNKKRGF"
     assembly_gap    3267..3366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3367..4322)
                     /locus_tag="P5179_08850"
     CDS             complement(<3367..4322)
                     /locus_tag="P5179_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS95363.1"
                     /translation="MRGEPVPLQPTRPNVSFAHQLWPGDCLTLLLAGFVASVLTGNFV
                     PRTNLSVLGTLVAMAGIIAVLLTSRAYEIQFPMAGTTSTYHAMRVLLWTVPPGGALWF
                     VFEPRVETAFAVFCYGIIAIFGMTLNRVIVFPRVRATAKSRVVVCGLNGDAVCVAQRF
                     ATDQDTMTPTIVGLLTPEAIENQPQRPRLGSWEDMQRLFSIGMFDEVVFSVPQSSPAS
                     LPLAATAVKHRIPFRFEPHERGSHFSPRDHSVDLTIEPPMGKAGRAIKRAVDLVGATL
                     LLVVTAPLYLAIAVMIKRDSQGPVLFAQKRVGLHGKLFTMYK"
     gene            complement(4579..4773)
                     /locus_tag="P5179_08855"
     CDS             complement(4579..4773)
                     /locus_tag="P5179_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95364.1"
                     /translation="MKLTREQLADLKGKSPEGIYQHIRQFVRQELGVVDKDELSEAVD
                     DAIRAGLLDEDDLRKLGDDR"
     gene            complement(4820..6112)
                     /gene="hflX"
                     /locus_tag="P5179_08860"
     CDS             complement(4820..6112)
                     /gene="hflX"
                     /locus_tag="P5179_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRS95365.1"
                     /translation="MKEYYYTSEASKPERAIVVGLQLGLQSAEEAQSSLEELEALADT
                     AGASVVARHFQHRAAPDPTFFVGKGKVGEIARDVEERQADLIIFDDDLSPAQARNVER
                     ECGIRVLDRSGLILDIFAKAAKTREARLQVECAQLEYLLPRLTRQWLHLERQAGTGGG
                     SSAAPIGLRGPGETQLETDRRLISARIAHLKRELEHIDRIRSTQRARRDRVYRCALVG
                     YTNAGKSTLMHALTGADVLIDDRLFATLDSTTRAFNLAPNKPVVLTDTVGFIRKLPHG
                     LVASFRSTLAEAREADLVLHVVDVSGPAAHEQIAAVNEVLSELDIHPSNTLLVMNKSD
                     ACVDPDDAARLAAGRPYVSVSALTGEGLDRLKEAIAANIEADMVLVEVRLPQSEGKLI
                     SEIHSRGEVLARTYEPDAVILHARVRRSDAEFLTRHSV"
     gene            6571..>6910
                     /locus_tag="P5179_08865"
     CDS             6571..>6910
                     /locus_tag="P5179_08865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRS95366.1"
                     /translation="MSERFETEFSVRSYELDAALDAPPASFLRWFQEAAIRASAAYGY
                     DEERYEQLGTAWFMKESHLLIRSQPRTGELVRVATWVGDITRVNSHRQYLAFGSDGRM
                     LARGEANWVYV"
BASE COUNT         1388 a         2091 c         1958 g         1373 t
ORIGIN      
        1 ggatgagttc ggaatacgac tcgggggagt cggggaacac ccacggcatg gtttcgagcg
       61 tgtagtaact acgcacgggt ttcactgcgt cgatgatctc tctgacggtc tgcacaatga
      121 ggtcgaacgt gtgtctcgtg aggttgtcgg gatgaggccc atcccactgc gttccccgtg
      181 atccggcgat gttcacgcag cagcgcgcgc cgattcggtc tgccagtgcc agctgatcct
      241 ggcactttcg gattgcggca tctcgcgtct ccgtatcagg gcttattgtg ttggaccacg
      301 caccagtttc agcgatcaca atgtcgaatt tctttgccgc ttccgcgtac gcgttgacga
      361 cgtcatccga agccgtgccg tccaccgggc agtacgccgc gcggtacccc gctatgccgt
      421 gtgctgcagc ccatgcttct ggcgttcctg cttcgtcgaa aaccggggca ccaagtcgca
      481 ttctcaggct cctgttgtct ggtcctggat caaagggtga aagtcgtcgt ccagcggaaa
      541 caccggcctt cgttggtggc ggtacgtgaa acgccggata tccagcgcgg tgattccggg
      601 tgtatcgagt tccaccatac gtccggcgat gggtgaatac gcagcccgat gtgcgattgc
      661 ggccttcacg acgattgtac ggcaccgggt cggctcgatg cccagagaac gaagctgctc
      721 cagattccac atctggtaac gcttctccgt cgcgatgatt gtcaccccgc gagcgtcgat
      781 cactgcggta cgccccatgt cctccctcaa tccctcccgc atcggaccga cgttcacaaa
      841 aagcccgctg gaaagaagcc tcacgtcgcc ttcagtttcc accggttccc cgtgtaatgc
      901 gtcggttttc cctccgatac gaaaacgtgc acggccgcgc actcccgttt caatcgccct
      961 ctgaacagtt tctgggtcgt gcaccaacac gacggctccc gaagagtgtt gttcaagcag
     1021 ctctttcaga acgatcgtcc cgtcccccgg tgctcctccc ccgacgttgt cgcccgtgtc
     1081 gaccagaaca accggagtcg gaccgggttc gttcgcctgt cggacggcat cctccacgga
     1141 taccaggtcc gtccggaagc tgtgccgcag gtcccacgcc ttccgggcga tggcctcgcc
     1201 agcacgcacg gtatccgggt cgcctttttc agataccgtg acgacggaaa ttccatggtg
     1261 aggaacgtct gcccattgga agccaccaaa cacgcttgca gagatacaac ccgcggcacg
     1321 gcggacgtcg tccgctgcgt ccatcaggca ccgcataggt gcgcaggcgg tgaactgcgt
     1381 cagcaagttg ggcaccaccg gtacctgaac gcagtgagca atcggatggg tctccccgcg
     1441 aagcgttttc gcaagtattt ccgccgcttc cttgccccgg tcacggtaat ccgtgtgagg
     1501 gtacgtgcgg tacccgacca cggcgtcaac tgcggcaacc atctctggcg tcacattggc
     1561 gtggaagtcc agtgtggcaa ctatcgggac gtgtcttccc acggccttcc gaacctggtg
     1621 caaccagaag gcgtccgcgt ctgtccgcgt ctcgcagacc atcgcgccgt ggcacacgag
     1681 aagaacgccc gcgatatcct gttcctgagc aatcctgcgg gccagttcac ctgcaagctc
     1741 ctccagatcg cgcgccgcaa ccgggcccga cggcatcccc cacgcgacaa gtgtgggaac
     1801 agcttccccg ccaatcgctt cgataccgtc cagaaatcct gcgacctctg agttcgtgcc
     1861 ccgccagtag tcaatgatgg caccaccggt cagcagactc gacgaccgga aatcctcgag
     1921 cgttgtcaac ctcgccgaga aggaattgga ttcgtgatag attccaccta tcgcgatacg
     1981 catgcgttct cccgcgtcgc caacggaaaa gcggcgctga aaagccttca gagccgcttc
     2041 cggtgagaaa tcgccagaaa tcaaaaaccg cgcttcttgt tcaccacgtt aaggagcggt
     2101 acaccccgaa cgaatctcca caggttctcc ctgagaatga cgaaccgcct tcgatctacg
     2161 gcgtcggtca ttccaccgat gtggggagtg atcgtgacgt tgggaagtgt ccagagaggg
     2221 tgatccgcgg gcaagggttc tggatccgtc acatcaagcc cggcgccacc gagatgtccg
     2281 ctgcgaaggg cctccacgag cgcatcagtg tccaccacgg gcccgcggga ggaattgatc
     2341 aggtatgcgc ctttcttcag gctctggagc cgctctcgat caaacagctt gcgcgcggtt
     2401 gttgccggag ccgtgatgaa gatcacgtcc gcgaggggca acacatcgtc gatccggtcc
     2461 gggcggacaa gttccactcc tgcggcagct ttcctgaccg ggtcaactcc gatgacgcgc
     2521 atcctgaagg ctttcgcacg gtccgcgacc tccgacccga tgttccccag ccctatcacc
     2581 accgccgtgg ccccgtacaa ttcgaatcct cgcagtgtgt cccggagcca ggtgaggcgg
     2641 tcaggccgca ccgtattggc gtgcacctga gtcgtcagag cgaggagatg ccccatggcg
     2701 agttcggcga tgaaaatgct gtacacgcct tttgagttcg taaggaccac gggactctcg
     2761 accatctcag ggaacagata cccctccaca cccgcagccg gtgaatggaa ccacctgagc
     2821 ctggctgctt tcgccaggat gtccttgtta atgccgccgt tcagggcgtc gagagatgcc
     2881 cagtcaatcc cttctctcga ttgaggtctg aatatcgtcg atcccggcgc ggcggcgcgg
     2941 agttcttcca tccagggttc tggccaccgc tcgaatgtga tcatcgtgat gggttctggc
     3001 gcatgcatcg gaagtgcccg tccttgaaag cgagtgagat ttgccggatg cggagtacgt
     3061 tcaaaatgtt cacgaagcgc agaaggggca agggtgcgag gtgagcgaca agccaaagcg
     3121 accgaggctc atacaccccg aaaggcgagg aaagccgtcc tcgtaagaat gacgaggtcg
     3181 agaatcaggg actggttttg cacgtaaaac aggtcgtagt ggatattctc gtgaatcgga
     3241 tcgttccggt gtctgctggt ctgccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnaact tgtacatggt gaacaacttg ccatgcaatc ccacgcgctt ctgtgcaaac
     3421 aggacaggcc cttgagaatc ccgctttatc atcacagcga tcgcgagata aaggggcgcg
     3481 gtaaccacca gcaacaacgt tgcacccaca agatcgaccg cgcgcttgat tgctctccca
     3541 gccttcccca tgggcggctc gattgtcaaa tccacggaat ggtcacgggg actgaaatgc
     3601 gagccccttt cgtgtggctc aaagcgaaat ggaatccggt gcttcaccgc cgttgcggcc
     3661 agcggcagtg acgcgggtga tgactgcggt acgctgaaca cgacttcgtc aaacatgcct
     3721 atcgagaaga ggcgctgcat atcctcccac gagcccagac gaggccgctg cggctggttc
     3781 tcgatcgcct ccggagtcag gagccctacg atcgtgggcg tcatggtatc ctgatccgtg
     3841 gcgaagcgtt gagcgacgca gacggcgtca ccattcaacc cgcagacgac caccctgctt
     3901 ttcgccgtag cgcgcacgcg ggggaatacg atcacccggt tcaatgtcat gccgaagatc
     3961 gcgataattc cgtaacagaa gactgcgaac gcagtctcga ctcgtggctc gaaaacgaac
     4021 cagagcgccc cgccgggtgg aacggtccac aggagcacac gcatggcgtg gtacgtactc
     4081 gtggttcccg ccatcggaaa ctggatctcg tacgcgcggg acgtgagcag gacggcgatg
     4141 atacccgcca tcgctacgag cgtgccgaga acactcaagt tcgtacgggg tacgaagttg
     4201 ccagtaagca cactggcaac gaaaccggcc agcaggaggg taaggcaatc acccggccaa
     4261 agctgatgcg cgaacgaaac gttcggacgg gtgggttgca ggggaacagg ctctccccgc
     4321 attctcccca gaatcccctt ggagggtcgt aaggcaatac ccggcacctg tgttacctgt
     4381 ctagttcgcg tgagcggccg agaaccaacc ggctgtattc cccgaaacca aaccggatgg
     4441 atcagtcaat cagtgacgat aaaaatacac gttttaagaa ttttgtcaaa agaaaccgac
     4501 atgtgaagcg gataggttcc aacacacgaa gagagatcgc agagtgatca ggcgtgtgtg
     4561 cgatatccag cgggaccgtc agcggtcgtc gcccagtttc ctcaggtcgt cctcgtcgag
     4621 aagacccgcc cggatggcat catccaccgc ctctgaaagt tcgtccttgt ccaccacccc
     4681 gagttcctga cggacaaatt gccggatgtg ttgatagatt ccttcgggcg atttcccttt
     4741 caaatcggca agttgttcgc gcgtgagttt catcaaggtc tcctgcactg aaagcgcgtt
     4801 gccatgtctg gtgttgcggt caaacggagt gacgagtcag gaactcggcg tcagagcggc
     4861 gtacacgggc gtgaaggatt actgcgtcag gttcgtacgt gcgagccagg acttcgcccc
     4921 gggaatggat ctcggaaatc aatttccctt cggactgggg aagccgaacc tccacaagta
     4981 ccatgtccgc ctcgatgttg gcagcgatcg cctccttgag ccggtccaat ccttctcctg
     5041 tgagagcact gacagaaacg tacggccttc cggcggccaa ccgcgccgcg tcatcagggt
     5101 caacgcaggc atccgatttg ttcatgacga ggagcgtatt tgacgggtgg atatccagtt
     5161 cggaaagaac ctcgttcacg gccgctatct gctcgtgtgc cgccggtccg ctcacatcca
     5221 ccacgtggag caccagatca gcttctctgg cctccgcgag cgtactccgg aacgacgcaa
     5281 ccagcccgtg cggaagcttc ctgatgaagc ccaccgtatc cgtaagcacc accggtttgt
     5341 tgggcgcaag gttgaatgcc cgtgtggtgg aatccagcgt ggcgaaaaga cggtcatcaa
     5401 tcagcacgtc cgcacccgtg agtgcgtgca tgagagttga cttgcctgcg ttcgtgtacc
     5461 cgaccagtgc gcaacggtac actcggtccc ttcgggcgcg ttgagtgctc cggattctgt
     5521 cgatatgttc aagttcccgc ttcagatgcg cgatgcgcgc gcttatcaac ctccggtcgg
     5581 tttccaactg ggtttcgccc gggccgcgaa gccctatggg agctgcgctg gaaccgcccc
     5641 ccgttccagc ttggcgctca aggtgcagcc actgacgcgt cagccggggc aacaggtatt
     5701 ccagttgcgc gcattcgacc tgcaggcggg cttctcgcgt cttcgccgcc ttggcgaaaa
     5761 tgtcgagaat cagcccgctc ctgtccagaa cccgaattcc gcactcacgc tccacgtttc
     5821 ttgcctgtgc cggcgagaga tcgtcatcga agatgatgag gtcagcttgg cgctcctcca
     5881 cgtcccgcgc gatctccccc accttgccct ttcccacgaa gaacgtcgga tccggcgccg
     5941 cccggtgttg gaaatggcgt gcaactacag aagctcccgc cgtgtcggcc agcgcttcga
     6001 gttcctcaag agagctctgt gcctcctccg cgctctgaag tcccagttgc agaccaacga
     6061 cgatcgcacg ctctggtttg gacgcttcag acgtgtagta gtactccttc acacaacctc
     6121 ctgaacgagt ttcaccggaa gcgagatcct tctttcccga gccgcgccgt tccgcctgtg
     6181 tttttacgcg gaattcggag gcaacagtgt cggcggatcc agggccacgg aaaggcatct
     6241 cgcgtatctt ggtatccctg ccgatccctg ccggccatat cggaaacaag aacgtacaaa
     6301 gcgtatcccc cattacaaga taagctcgcg aacctgcctg cgcgcatccg agggacggca
     6361 acagatccgg cgattgatta cgaaggatag catgtggcgt accctggcgt tcggcggcga
     6421 tagtgtcttc gttctcgcaa aaggacccgc tgagcgaccc cggccattga tcggggccca
     6481 ttcgggggta tgacattcgg taccatttga acgaccttcg gtacagacgt atggcactat
     6541 gagtgtgttg aatgaaacga ggctgcacgc atgtctgagc gattcgaaac ggagttttcc
     6601 gtccggagct atgaactgga cgcagcactg gacgctccgc ccgcttcgtt ccttcgttgg
     6661 tttcaggaag cggcaatccg tgcctcagcg gcgtacgggt acgacgagga acggtacgaa
     6721 caactgggca ctgcctggtt catgaaagaa tcccacctgt tgatccgttc tcagccccgt
     6781 acaggagaac tcgttcgagt agccacgtgg gtaggcgaca taacgcgggt caactcccat
     6841 cgccagtatc tcgccttcgg atctgacggg agaatgttgg cccgcggaga agcgaactgg
     6901 gtgtacgtgg
//
LOCUS       DAOTNG010000166         6877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9567_length_6877_cov_5.390501, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000166 DAOTNG010000000
VERSION     DAOTNG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9567_length_6877_cov_5.390501"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="P5179_08870"
     CDS             complement(<1..694)
                     /locus_tag="P5179_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS95367.1"
                     /translation="MSIETLTTTFAEGRAVTIPREMLEHLKELVGARFVLNHATEMLP
                     YEADGLTEFRTPPALVVLPKTAEQVQECVKTCVRFGFPFTARGAGTGLSGGAVPAPGG
                     VVISLSRMNRILEVNYQDRTAIVEPGVLNLELSELTSPQGFVFMPDPASQPACTLGGN
                     IGENAGGPHCLKYGVMVNHVRALEVVTPTGDLVTLGGAGGDAVGYDLMGLFIGSEGTF
                     GIATRLFLRLEPT"
     gene            complement(684..1388)
                     /gene="lepB"
                     /locus_tag="P5179_08875"
     CDS             complement(684..1388)
                     /gene="lepB"
                     /locus_tag="P5179_08875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRS95368.1"
                     /translation="MQPDEPSETASKETTPWRVYAAVLSASLLGALLLRAFVIHAYRI
                     PSGSMESTLLVGDYLLAERLTFGSFVELPVARKELFRWPPLRRPRRGEVVIFRSWGDP
                     GTEIVKRCVGLPGDTVEVVDNIVKVNGVASDPIFQGYFDTTHKVNTEDVFPVSEWRAV
                     ERARNFGPHIVKPGHIFVMGDNRTNSEDSRVHGDVSFEALTGRPLFIYWSVNSHSASL
                     NPFDSIRWERIGKRVH"
     gene            complement(1393..2139)
                     /gene="lepB"
                     /locus_tag="P5179_08880"
     CDS             complement(1393..2139)
                     /gene="lepB"
                     /locus_tag="P5179_08880"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRS95369.1"
                     /translation="MDKPPVVPSSVVSGSGEKSATRKSLGKEYVQPLLTVLLVALVIR
                     TFVIHAYRIPSGSMEDTLLVGDFLLANRFVYGSPIEIPFTGIVLGRLPAISSPQRGDV
                     VIFASWADTTEDFIKRCVGLPGDTILVRNNVLTVNGTPFSQTLRARFGNDWRVFPSIK
                     YTPTHYLGMSGIMALDPTNFGPHVVPPGHMFVMGDNRNNSADSRFYGDVPLNRLKARA
                     LVIYWSMDTRQRLWKLSEYIRWDRIGRLIR"
     gene            complement(2158..3963)
                     /gene="lepA"
                     /locus_tag="P5179_08885"
     CDS             complement(2158..3963)
                     /gene="lepA"
                     /locus_tag="P5179_08885"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRS95370.1"
                     /translation="MTDQKRIRNFSIIAHIDHGKSTLADRLLEFTGTLSSNEMQDQVL
                     DSMDLERERGITIKMHAIRMEYGAENGETYVLNLIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDAAQGVEAQTVSNLYQAISADLEIIPVINKIDLPSAQIPLVTQEIRDLLGVEEKD
                     ILLVSAKTGQGIRELLEAIVAHVPEPKGDPDAPLRALIFDSVYDSYRGAVVYVRVVDG
                     AIRPGQKVRLAATNAVHDVDEVGFLRLGRVARDSLQAGEVGFVVASIKTISEARVGDT
                     ILDNSAPAVVPLKGYQEVKPMLFAGIYPVDAEMYEDLRDSLARLKLNDASLVYEPESS
                     AALGFGFRCGFLGLLHMEIVQERLDREFGLNIITTVPNVRYRVVTTSDEVIWVDNPSK
                     LPDPARIDRIEEPFLLTQIVTPTDYIGPIMKLATERRGVYETTEYLSPKTVDMRFQMP
                     LSEIVFDFYDKLKSQTRGYASFDYEFAEYRPSKLVKLDILINGNPVDALSVIVHADKA
                     FAWGRNFCLKLKHLIPRQLFEVVIQGAIGSRVIAREVVRPLRKDVTAKCYGGDITRKR
                     KLLEKQREGKKRMKQVGSVEIPQEAFLAVLQMDPG"
     gene            complement(4079..4657)
                     /locus_tag="P5179_08890"
     CDS             complement(4079..4657)
                     /locus_tag="P5179_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRS95371.1"
                     /translation="MGFVATFFIALGLSADAFAVAVSNGFSSRRPRIGEAFRFGVAFG
                     SFQALMPLIGWLAGSSMAGLFEDTDHWVAFGLLAFIGGKMVYEGIWGEATNGEGKPVT
                     LWVLLILSVATSIDALAVGISLSLLSTPIFMPAMLIGVTTLCVSTAGFLLGKRLGRFF
                     GRRVEVAGGIILVAIGAKILIDHLTRGVGGQV"
     gene            complement(4778..5167)
                     /locus_tag="P5179_08895"
     CDS             complement(4778..5167)
                     /locus_tag="P5179_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95372.1"
                     /translation="MENLVSNLLSWVSSFFGNLYADAPIIWGSKYPAFLGELLLLIIV
                     IDLVIFGWRPLVKRFFPKSYGAVDYAFGQVTNLIWLCILVAFMIYIGGALGEAWGRFL
                     GVSTMIWSTVGVVALLLLGKLAVKRHR"
     gene            complement(5276..6628)
                     /locus_tag="P5179_08900"
     CDS             complement(5276..6628)
                     /locus_tag="P5179_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRS95373.1"
                     /translation="MSEGLWASARSRLWCNDTRLAESTWGRVLLRLLRTSVIAGKGFW
                     NDECMLRATALAYTTLLSLVPLLAVAFSLFAAFPELENAYAEIRSFLNEYLAPGASKA
                     AISALNEFIANATTGTLAGIGTAALFVTVFLMLSSVEESLNRIWRAPKTRPILDRLIY
                     YLAIVILGPLFLAVSVNAAIQTVQRESLALNLVEPPFVSELWEYSLPLLASCALFTIL
                     FKAMPNTKVRWPAAVAGGIVSGVLFEIAKKAYTLYASNAIAYRTIYGAMSTVPLFLVW
                     IYVIWLVVLFGAEFAHGWQTIISYQRIATWGPTSQYHRELLAAALAVATTRRFEEGRP
                     GLTSQEMADIFDAPLGIVEEIVEILKSSSVLTESGRGILPAKDPDKLTLWDVVGPLRK
                     GAETRPIPEQLFREEVGPAVLFLEEVETGNEEPFRSITLAELAAKSAVAETTPDERGQ
                     "
BASE COUNT         1554 a         1984 c         1897 g         1442 t
ORIGIN      
        1 gggtgggctc aagacgcaaa aacaaccggg tagcgattcc aaaagtgcct tcggagccga
       61 tgaagaggcc cataaggtcg tatccgacgg catcccctcc ggcgcctccg agtgtcacca
      121 ggtcacctgt cggggttacc acctccagcg cccgcacatg gttcaccatg accccgtatt
      181 tcagacaatg cgggccgccc gcgttctctc cgatgttgcc accgagtgtg caggcgggct
      241 gactcgcggg atccggcata aacacgaatc cttgcgggga ggttaactca ctgagctcga
      301 ggttgaggac tcccggttcg actattgccg ttcggtcctg gtaattcacc tccaggatgc
      361 ggttcatgcg gctgagagag atcactacgc ctcccggagc cggaaccgct cctccgctca
      421 accccgttcc tgctccacgt gccgtgaagg ggaagccgaa ccggacgcac gttttcacgc
      481 attcctgaac ctgttctgcg gttttcggga gcacgacaag cgcgggtgga gtgcggaatt
      541 cggtcaaccc atcggcttcg tagggtaaca tttccgtggc gtggttgaga acgaatcttg
      601 cccccaccag ttctttgagg tgctccagca tttccctggg aatcgtcacg gcgcgtcctt
      661 ccgcgaaagt tgtcgtgagg gtttcaatgg acacgtttgc ctatcctttc ccatctgatg
      721 gaatcgaaag gattcagtga tgcggaatgc gagttgactg accagtaaat gaacaacggc
      781 cgcccggtca aagcttcgaa ggagacatcg ccgtgaacgc ggctgtcctc cgagtttgta
      841 cggttgtcgc ccatcacaaa aatgtggccg ggtttgacga tgtgcggccc gaaattgcgc
      901 gcacgttcta ctgcccgcca ctccgacacc gggaaaacat cttctgtgtt gaccttgtgc
      961 gtcgtgtcga agtagccctg gaaaatgggg tccgacgcta ccccgttcac cttcacgatg
     1021 ttgtctacca cctcaaccgt gtcccccggc agacccacgc accgtttcac gatttcggtt
     1081 ccggggtcgc cccacgagcg aaagatgacg acctcacccc gtctcggtcg ccgcaggggc
     1141 ggccagcgaa acagctcctt cctcgcgacg ggcaattcca caaatgagcc gaatgtcaga
     1201 cgctccgcca ggaggtaatc gccaacaagc agtgtgcttt ccatggagcc ggaagggatg
     1261 cggtacgcgt gtatgacgaa ggcgcgaagc aacaatgcgc caaggagcga cgcagaaaga
     1321 accgctgcat aaacccgcca gggtgttgtc tctttggacg ccgtctcaga gggttcgtct
     1381 ggttgcatgg ggtcagcgga tcagtctgcc gattcgatcc cagcgaatgt attcggagag
     1441 tttccacagc cgttggcggg tatccatgga ccagtaaatc acaagcgcac gcgccttgag
     1501 ccggttgagc gggacgtcgc cgtagaagcg actatcagcg ctgttgtttc tgttgtcgcc
     1561 catgacaaac atgtgcccgg gtggaacgac gtgcggacca aagttggtcg gatccagggc
     1621 catgatgccg gacattccga gataatgcgt cggtgtatac ttgatgctcg gaaagacacg
     1681 ccaatcgttg ccgaagcgtg ctctcagggt ctggctgaag ggcgtgccat tgaccgtgag
     1741 aacgttgttc ctgacgagaa tcgtatcccc gggtaaccca acgcaccgtt tgatgaagtc
     1801 ttcggtcgta tccgcccagg aagcgaaaat caccacgtct ccgcgctgcg gagatgatat
     1861 tgcaggtagt cttcccagca cgattccggt gaaggggatc tcgatcgggg agccgtacac
     1921 gaaccggttc gcaaggagga aatccccgac cagaagagtg tcctccatcg atccggatgg
     1981 aatgcggtat gcgtggatca cgaacgttcg tatcaccaga gcgaccagaa gcacggtaag
     2041 caaaggctgt acgtattcct tgccaaggga tttcctggtt gccgattttt cccccgaacc
     2101 ggacacaacg ctgctgggga ccactggtgg tttgtccact gcctgctcct tctcttttca
     2161 accggggtcc atctgaagga ctgcgaggaa agcttcctgg ggaatctcta cacttccgac
     2221 ctgtttcatc ctttttttgc cttctctttg cttttcgagg agtttgcgct tacgtgtgat
     2281 atccccgccg taacacttcg ccgtgacatc ttttcgaagt ggccggacta cttcacgggc
     2341 gatcacgcgg ctgccgatcg ctccttgaat gaccacttcg aacaattgac gcgggatcag
     2401 atgcttcagt ttcaggcaaa aattccttcc ccacgcgaac gctttgtccg catgcacaat
     2461 gacactcagc gcatcaacgg gatttccatt tatcaggatg tccagcttga cgagtttcga
     2521 cgggcggtac tctgcaaact cgtagtcgaa agaagcatac cctctggtct gggatttcag
     2581 cttgtcgtag aaatcaaaca cgatttcgga aaggggcatc tggaatcgca tatcaacggt
     2641 ttttggcgaa aggtactccg ttgtctcgta cacgccgcgt ctttcggtcg cgagtttcat
     2701 gatggggccg atatagtccg ttggcgtaac gatctgcgtg agcaaaaacg gctcttctat
     2761 tcggtcaata cgagccggat ccggcaattt ggagggattg tccacccaga ttacctcgtc
     2821 ggacgtcgta acaacgcggt accgcacgtt tgggacggtc gtgatgatgt tcaggccgaa
     2881 ctcccggtcg agtcgttcct gaacgatctc catatgcaag agaccgagga acccgcaacg
     2941 gaagccaaaa cccagagcag cagagctttc cggttcgtac acgagcgacg catcgttgag
     3001 tttgagacga gcgagggaat cccgcagatc ctcgtacatt tccgcgtcca cgggatagat
     3061 gcccgcaaac agcatgggtt tgacttcctg gtagcccttc agcggaacaa ccgcgggagc
     3121 ggagttgtcc aggatcgtgt cgcctacccg tgcctcgctg atcgttttga tcgacgcgac
     3181 cacgaacccc acttcaccag cctgaaggga atcgcgggcc acgcgtccga gcctcaggaa
     3241 gccgacctcg tcgacatcgt gaacggcgtt cgttgcggca agtcgaacct tctgtccggg
     3301 gcgaatcgcc ccatccacga cacggacata cactacggcg ccgcggtaag aatcgtagac
     3361 agagtcgaat atgagagcac gcaatggcgc gtccggatca cctttgggtt cggggacatg
     3421 ggcaacgatg gcttcaagca gctcgcggat tccctgccct gttttcgccg aaaccaggag
     3481 tatgtctttc tcttcgacac cgagaagatc cctgatttcc tgagtaacga gcggaatctg
     3541 tgcgcttggc aaatcgatct tgttgattac cggtatgatc tcaaggtccg cggagatcgc
     3601 ctggtaaaga ttgctgacgg tctgtgcttc cacaccttga gcagcgtcga caacgaggat
     3661 cgcaccctcg caggccgcaa ggctgcgtga cacctcgtac gagaaatcca cgtgtcccgg
     3721 ggtgtcgatg aggttcagga cgtacgtctc gccgttctcg gcaccatatt ccatccggat
     3781 tgcatgcatc ttgattgtga tgccgcgctc ccgttccagg tccatgctgt ccagaacctg
     3841 atcctgcatc tcatttgagg aaagcgtgcc ggtgaattca agcaggcggt ctgcgagggt
     3901 tgatttaccg tggtctatgt gggcaattat ggagaaattt cgaatgcgtt tctggtctgt
     3961 cattcttttc ccatgcgggt gttcgcagaa catactgatg aagggatacc tgaaaggaga
     4021 catccgaaag atacaaaaaa gcgggccaga accgtcggat acacgtgtcg catgtggttc
     4081 agacctgccc tccgacacca cgtgtcaaat gatcgataag gatcttcgcg ccgatcgcga
     4141 ccaggataat gcctcccgcg acctcaactc tccgcccgaa gaagcgaccg aggcgtttcc
     4201 ccagaaggaa gccggcagtg gacacgcata atgtcgtaac gccgataagc atcgcgggca
     4261 tgaatatcgg cgtgctgagc aacgaaagac ttattcccac tgccagggca tctatgcttg
     4321 tggctaccga cagaatcagc aacacccata aagtcacggg ctttccctcg ccgttcgtcg
     4381 cctctcccca gattccctcg tacaccattt tcccgccgat gaacgcaagg aggccgaatg
     4441 caacccagtg gtcagtatcc tcgaacaaac ctgccatgct cgagcctgcg agccatccta
     4501 tcaaaggcat gagtgcctga aacgatccga aggcgacgcc gaaacgaaac gcttcgccaa
     4561 tccgcggtct gcgggacgaa aatccgttcg aaacggcaac agcaaacgcg tccgcggaaa
     4621 gtccgagcgc tatgaaaaaa gtggcaacga aacccatagg cacaccaggg atgggataac
     4681 gagcgtggtt gtcagcgcca aaaggggaac aaaaaaccgg tgggggtagt gaagccaccg
     4741 gtttccttgt atggaacgtg cgtattcccg ctgccggcta cctgtgccgc ttcactgcca
     4801 gtttgcccag gaggaggaga gcaaccacgc cgaccgtcga ccagatcatt gtggatacgc
     4861 caagaaatct gccccaggct tccccgagcg caccgccgat ataaatcatg aacgcaacaa
     4921 gaatgcacag ccagatcagg tttgtcacct gcccgaacgc gtaatcgacg gcgccatagg
     4981 acttggggaa aaacctcttc accaacggcc gccacccgaa aatcaccagg tcgatcacga
     5041 tgataagcag aagcagttcc ccgagaaatg ctgggtactt cgatccccag atgatcgggg
     5101 catcggcgta caggttcccg aagaacgaag agacccaact cagaaggtta ctcaccaagt
     5161 tttccacgga tcgtcctcca aaagagggtg acgtgcaatg attactacca aaggtgaagt
     5221 tctggtttgc ccggaatact aataatacaa gcggggatac gaccgcgcaa caccgtcatt
     5281 gtccgcgttc gtcaggagta gtttctgcaa cggcagattt ggctgcaagt tccgcaagcg
     5341 taatggatcg aaacggttct tcgttgccgg tctcgacctc ttcgaggaac agaacagcgg
     5401 ggccgacctc ctcacgaaaa agctgctcgg gaatcggcct ggtctccgca cctttcctca
     5461 agggcccgac cacgtcccag agtgtcaact tgtccgggtc cttcgccgga aggataccgc
     5521 gccctgattc cgtgagaact gaactcgatt tgaggatctc gacgatctcc tcaacaatcc
     5581 cgagcggcgc atcaaaaatg tctgccattt cctgagacgt cagacccggt ctgccttcct
     5641 caaaccttct ggtggtggca acagcgagcg ctgcagccag aagctctcga tggtactgac
     5701 ttgtcgggcc ccatgttgcg attctctgat agctgattat agtttgccaa ccatgggcga
     5761 actccgcgcc aaaaagaacg acgagccata ttacgtatat ccacacaagg aacagcggca
     5821 ctgtactcat cgcgccgtag atagtcctgt acgcgatggc gtttgacgcg tacagggtgt
     5881 acgccttttt ggcgatttca aacagcaccc cggagacgat tcccccggca accgccgctg
     5941 gccaccgcac tttcgtattc ggcatcgctt tgaacagtat cgtaaacagc gcgcaggatg
     6001 caaggagcgg cagcgaatac tcccacaact cggacacgaa tggaggctcg accagattga
     6061 gcgccagtga ttcccgttga accgtttgaa tcgcggcgtt cacagatacc gcgaggaaga
     6121 gagggccgag gatgacgatt gccaggtagt agatgagcct gtccagaatc ggtcgtgtct
     6181 tgggcgcccg ccagatccgg ttcaatgact cctcgacgga gctcagcatg agaaacacgg
     6241 tgacgaaaag tgccgcagtt ccgattcccg cgagcgtacc ggtggttgca ttcgctatga
     6301 attcgttcag tgcggaaatg gcggccttgc ttgctcccgg tgccaggtac tcgttcagaa
     6361 aggagcgtat ttcggcgtag gcattctcaa gctccgggaa agctgcgaaa agtgaaaacg
     6421 ccacagccag aagcggaacc agggaaagga gcgtggtgta tgccagtgca gtcgcccgca
     6481 gcatgcattc gtcgttccag aaccccttgc cggcgatcac gctggtgcgc aggagtcgca
     6541 agagtactct gccccaggtg ctttccgcca accgcgtgtc gttacaccac aatctggaac
     6601 gagcggaagc ccacaggcct tctgacaatt gccgacctcc ttattttgtc ttcgggcggg
     6661 agccgtcaca cagccgccta tatggtactc aaaaccggtt ccactgacca cggcatcatc
     6721 cgatcgattc gggcggcaca cctgcagacg ttctcataac tgctcgcacc tgctatcttt
     6781 ttaggtgccc gttgccgcag ccgtggcgcg aatccgtcta gctgaattgg aacccctcga
     6841 ggttgtcgtg gattctgaaa aaccagatcc gaggccc
//
LOCUS       DAOTNG010000167         6831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9645_length_6831_cov_5.681523, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000167 DAOTNG010000000
VERSION     DAOTNG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9645_length_6831_cov_5.681523"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="P5179_08905"
     CDS             complement(<1..1144)
                     /locus_tag="P5179_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95374.1"
                     /translation="MELVWAPERIGEGRRFRVIIRSDAPVRCFYSKHLELADRTPEAR
                     NSLYFLYFRSVIPTTAAEIRCESGSESIVVRLPVIAEADWEHDARLHEIPLPRIWPME
                     RTYFPLKNRHTLVDGNEIGVRNSETQVSVNEGHAVDSAGSARRKQGDRTSCEALEWPD
                     EEIWNLEPQCDIPRWHFLNLDKGCPVHGLAIYSHNAYYPWIVDERNRPYHVKCPVGGE
                     WYPTNNLAEGDHTGGAFPDDGWGWHSPDGAVFGFTAYALLRRIRLVYGTVQSLAQHFK
                     GTGDQKAARKIVYLLISIAREHRYLAFFPEHRFRKYEEVVEEPHYREHKDRISWGPRE
                     TRTVANLAGGSGMDEYCINMPYHYEHLSRAYDMVFDRIDGDSELIAF"
     gene            complement(1152..2351)
                     /locus_tag="P5179_08910"
     CDS             complement(1152..2351)
                     /locus_tag="P5179_08910"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRS95375.1"
                     /translation="MHVLVVNVGSTSFKYRLLAMPEERVLAEGRLERIGSASSPVEFS
                     SGEHSEKSTIYLPDYAAAIGKMMSDLTSAKSPIESLKQIDAVGFKTVVIDGVPGCVLL
                     DDDAVSRMEALNDIAPAHNPPYVNAVRQMRALFPDLPLVGLFEPAFHVTMPDYAYTYG
                     VPHSWAEKYGVRKFGYHGASHRYVAETAPKLAGIDPSRCRLISAHLGGSSSLCAIRNG
                     QSIDTSMGMSPQSGVDHSTRNGDLDVFAVLHVMDKEGLSTSDVRRILCKEAGLAGISG
                     TSGDVRDLEAAAASGNDRARLALDVFCYNAKKTIGAMAAALGGLDVIGLAGGIGERGV
                     NVRKRILNGLDFLGVALDEERNSAHTGQEGVISTDASPVKVVVVRTNEELIVARSVFA
                     LLSGNAS"
     gene            complement(2450..3262)
                     /locus_tag="P5179_08915"
     CDS             complement(2450..3262)
                     /locus_tag="P5179_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRS95376.1"
                     /translation="MAIPLFQLKQQIVDACHRVYAKGWVASNDGNLSVRVDGERVLCT
                     PTGISKGLVTVDDLCLIDMEGNKLEGAAHRKPSTEMKVHLMAYKERPDVVSVVHAHPP
                     YATGYALAGLDLGECKLPEVVIALGTIPLTKYGLPGTDDLPNKMREFIHQSDAFLMEL
                     HGVLTVGQSVLNAYFKMETVEHFAKIDFIARQLGGARLFSKEQAEELLAARARYGVTT
                     PNIGCRAADGYAYPPDARDNLGPGANPDSGIPEDKLVEEVTKRVVQALKAKN"
     gene            complement(3286..3585)
                     /locus_tag="P5179_08920"
     CDS             complement(3286..3585)
                     /locus_tag="P5179_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRS95377.1"
                     /translation="MIVGRVVGSVVSTVKHPAYASTKLLLVRPVSMDGVEEGPEMVCV
                     DTVGAGAGELVLVCKEGAAARQVLRKKHAPVRSLVIGIVDHVEAADGRRMIDGRD"
     gene            complement(3582..3884)
                     /locus_tag="P5179_08925"
     CDS             complement(3582..3884)
                     /locus_tag="P5179_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRS95378.1"
                     /translation="MQLGRIIGTIVATQKIERLCGVRLVVLEPLNARREPTGPPIAAI
                     DVVAAGVGDTVFWVGSLEAAQALEPPFPAVDASVVGLVDRVDLLPGKPASEETGAE"
     gene            complement(3906..5369)
                     /locus_tag="P5179_08930"
     CDS             complement(3906..5369)
                     /locus_tag="P5179_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HRS95379.1"
                     /translation="MQIDEARVRAIVEQVVKQLGTPAGATGAKTPVNAPDSGAVNPPA
                     TTLGRGVFADIDEAVAATEKAQREWIAMPVAKRKEVVEAVRRVGIEHGEMFSRETVAE
                     TGMGRVADKIAKHRLASLQTPGFEDLVTTARSGDHGLTIEEMAPYGVIAAVTPSTHPV
                     PTMINNTICNLVAGNGVVFNGHPAGKRVFAHALQVINEYVVAAGGPANIITTVAEPTI
                     EGANKLFHHPAIKLILVTGGPGVVKAAMGAGKKVIAAGPGNPPVVVDETADIEKAARS
                     IIAGATFDCNLLCIAEKEIFVVDTVADELKRRMVANGAFELTTAQIQQLTSFVFKGDA
                     GCAHPVLNRDAVGRDADTLAKAIGLSVPSTTQLLIGETTKEHPFVQHEQMTSFVPIVR
                     CRDVHQAIEWAYEAEHGFGHTAIMHSKNVENMDKMAKVMNTTIFVKNGPSTAALGSGG
                     EGTSSFSIALATGEGITTPRTFTRIRRCALVDYFRII"
     gene            complement(5421..5720)
                     /locus_tag="P5179_08935"
     CDS             complement(5421..5720)
                     /locus_tag="P5179_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRS95380.1"
                     /translation="MILGRVAGTVVATQKDEKLRSAKLLVVETLDPLTGEGKKAYTVA
                     IDAVGAGLGEVVLCASGSSARLTPSSDGRPVDTVIMAIVDQYDIHGKIVYRKSGQ"
     gene            complement(5909..>6831)
                     /locus_tag="P5179_08940"
     CDS             complement(5909..>6831)
                     /locus_tag="P5179_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRS95381.1"
                     /translation="KRLDPWRPCIPSSPYYSAESREHGTVEVMPERHLWGPRDYYKSR
                     FYAENRAHFVSEIGYHGCPSQSSLEKFLSPEALWPPEGNEEWRIHAADTTPSPGAYAY
                     RIALMSRQVAEVFGRTPETLDDFVVLSQIVQAEAKKYFVEMTRLRKWRKTGVLWWNLI
                     DGWPQFSDAVMDYFYCPKLAYFYLRRVQQPVCVMVDEPEDWRCRVVLGNDSLERVQGT
                     FRIWDAATGETIWEGGGTPERNANTVLTAIPASRGQQRLLLIEWETEGIKGGNHYLLG
                     SPPFNGTWYRELLPAIAALPCGFDSLAVGK"
BASE COUNT         1315 a         2210 c         1840 g         1466 t
ORIGIN      
        1 caaacgctat cagctctgaa tcgccgtcaa ttcggtcgaa caccatatcg tatgcacggg
       61 agaggtgctc atagtggtac ggcatgttga tgcaatactc gtccatgccg gagccacctg
      121 cgaggtttgc gaccgttctc gtctcccgcg gtccccacga gatccggtcc ttgtgttccc
      181 ggtagtgcgg ctcttccact acctcctcgt atttgcggaa gcggtgttct ggaaaaaacg
      241 ccagataacg gtgttcacga gcgatactga tcagcagata cacgatcttt ctggccgcct
      301 tctggtctcc cgtccccttg aaatgctgcg cgagagactg cacggtgccg tacacgaggc
      361 ggatccggcg gagcaacgcg taggcggtga agccgaaaac ggcaccgtcg gggctgtgcc
      421 aaccccaccc gtcgtccgga aaggcaccac cggtgtggtc gccttcggcg agattgttgg
      481 tggggtacca ttccccacca accggacatt tcacatgata cggccggttc cgttcgtcca
      541 cgatccacgg gtaataggcg ttgtgcgagt agatcgcaag gccgtgtaca gggcagcctt
      601 tgtcgaggtt tagaaagtgc caccgtggaa tgtcgcactg cggttcgaga ttccagatct
      661 cttcgtcggg ccactccagc gcttcgcatg acgttctgtc accctgtttt cgtcgtgcgc
      721 ttcccgcgga gtcaacggcg tggccttcgt tcaccgaaac ctgcgtctca ctgttccgaa
      781 cgccgatttc gttcccgtca accagcgtgt ggcggttttt cagtggaaag tacgtcctct
      841 ccatcggcca gatgcggggc agcggtatct catgaagcct ggcgtcgtgc tcccagtctg
      901 cttccgcaat gacgggcaat cgcacaacaa ttgactcgct tccgctttcg catcggattt
      961 ccgccgcggt ggtcggaatt acgctccgga aatacaagaa atacagtgag ttgcgcgcct
     1021 caggcgttcg atcagccagc tcgaggtgct tggaatagaa acaccgtacg ggtgcatccg
     1081 atcgtatgat taccctgaac ctgcgccctt cgccgattct ttcaggcgcc cagacaagtt
     1141 ccatactgcc ctcacgaagc gttcccgctc agaagtgcaa acacagaccg cgcaacgata
     1201 agctcctcgt tcgtgcggac gacgacgact ttgaccggtg aggcgtctgt ggagatcacc
     1261 ccttcctgcc cggtgtgcgc agagttcctc tcctcgtcca gggccactcc gaggaaatcg
     1321 agcccattga gtatgcgttt tctcacattg acgccacgct ccccgatgcc gcccgcgagc
     1381 cctatcacgt ccaggccacc cagagccgcc gccatcgcgc cgatggtttt ttttgcgttg
     1441 taacaaaaca cgtccagtgc gaggcgcgca cggtcgtttc cactggcggc cgccgcttcg
     1501 agatcacgca catcaccgga ggtcccgctg atgcccgcga ggcccgcctc tttgcacaga
     1561 atacggcgca cgtcactggt actcaagcct tctttgtcca tcacgtggag cactgcgaac
     1621 acatcgaggt ctccgttccg cgtggaatga tccaccccac tctgagggga catgcccatg
     1681 gacgtgtcaa tcgactggcc gttgcgaatt gcgcagagag aagatgaccc cccgagatgg
     1741 gccgaaatga ggcgacagcg ggaggggtcg atacctgcga gcttgggagc cgtttcggca
     1801 acataccgat gggaagcgcc atggtacccg aatttccgaa caccatactt ctcggcccag
     1861 gagtgcggaa caccgtaggt gtacgcgtaa tcaggcatcg taacgtgaaa tgcgggttcg
     1921 aacagcccaa ccaggggaag gtcagggaaa agcgctctca tctggcgaac cgcgttcacg
     1981 tacgggggat tgtgcgcggg ggcgatgtcg ttcaaggctt ccatacgact cacggcgtcg
     2041 tcgtccagca ggacgcagcc gggaacgccg tcaatcacca ccgtcttgaa ccccaccgcg
     2101 tcaatctgtt tcagcgattc gatcgggctc ttcgcggagg tcaggtcgga catcattttc
     2161 ccgatggctg ccgcgtagtc cggaagatag atcgtgctct tttccgaatg ctcgccgctt
     2221 gagaactcca cgggagaaga tgcgctgccg atccgttcca agcgaccttc cgccagaacg
     2281 cgctcttccg gcatcgccag gaggcggtac ttgaacgacg tgctgccaac gtttacgacc
     2341 agaacatgca tcctgcctcc ctccaacccg tcgcaaaatc aaacgggcgc attaccgtgc
     2401 gcccgccgta cagaaactgg cccgccatat ctggttcacg tgccacaact tagttcttgg
     2461 ctttgagcgc ctgaaccact cgcttcgtga cctcttccac gagcttgtcc tcagggattc
     2521 cagagtcggg attggcaccc ggtccaaggt tatcccgggc atccggcggg taagcatacc
     2581 cgtccgcagc ccgacacccg atgttcggcg tcgtgacgcc gtatcttgcc cgtgcggcca
     2641 gaagttcctc ggcctgttcc ttggagaaca gacgcgctcc accgagctgg cgggcgatga
     2701 aatctatttt ggcgaaatgc tccaccgttt ccatcttgaa atacgcgttg aggacactct
     2761 gtcccaccgt cagcaccccg tggagctcca taaggaaggc gtccgactga tggatgaact
     2821 cgcgcatttt gttcggcaga tcgtccgttc ccggcaatcc gtacttggtc aaaggtatgg
     2881 ttcccaacgc aatgacgacc tccggaagct tgcactcccc gaggtcgagt cccgccagag
     2941 cgtacccggt tgcgtacggc gggtgggcat ggaccacgga aaccacatcc ggacgttcct
     3001 tgtacgccat caggtgaact ttcatttctg tcgacggctt acggtgcgct gctccttcga
     3061 gcttgttgcc ttccatgtcg atcaggcaca ggtcatccac ggtgaccagc cctttgctga
     3121 ttcctgtcgg tgtgcagaga accctctcac cgtcgacccg tacgctgagg tttccgtcgt
     3181 tcgacgcgac ccaacctttg gcgtacacgc gatggcacgc atccacaatc tgttgtttga
     3241 gctgaaaaag cggtatcgcc atggttatgt ccctgttgcg tatctctaat ctcttccgtc
     3301 gatcatgcgc cgtccgtccg cggcttccac gtgatcgaca atccctatca cgagcgacct
     3361 tacgggcgcg tgcttcttcc gtagcacctg tcgcgcggct gcgccctcct tgcagaccag
     3421 taccagttcc ccggcgcccg ctcctacggt atcaacacac accatctccg ggccctcttc
     3481 gaccccgtcc atgctcacgg gcctcaccag aagcagtttt gtggaggcgt acgccgggtg
     3541 cttaactgta gaaacaacac tccccaccac ccgtccgaca atcattccgc ccccgtttct
     3601 tcggatgcgg gtttccccgg gagcagatcc accctgtcca caagccccac aacgctcgcg
     3661 tcgaccgccg ggaatggcgg ttcaagcgcc tgcgcagctt ccaacgaccc aacccagaac
     3721 accgtatcgc caactcccgc cgccactaca tcgattgcgg cgattggcgg ccctgtcggt
     3781 tcgcgccgcg cgttgagcgg ctccagaacc acaagtctca cgccacagag gcgttctatt
     3841 ttctgggttg cgactatggt tccgattatg cgacccaatt gcatccgtct gccgcttgcg
     3901 agagttcaaa tgatgcggaa ataatccacg agggcgcacc gacgaatgcg cgtgaacgtt
     3961 cgcggcgtcg taatgccttc acccgtcgca agcgcgatgg agaatgacga ggtgccctct
     4021 ccaccggagc ccagcgccgc cgtgctgggt ccatttttca cgaagatcgt tgtgttcatg
     4081 actttggcca tcttgtccat gttctcgacg tttttcgagt gcatgatcgc ggtgtggcca
     4141 aaaccgtgtt cggcttcata ggcccattcg attgcctgat gcacgtcgcg gcagcgtacg
     4201 atgggaacaa aggaagtcat ctgctcgtgc tgcacgaacg ggtgctcctt ggtggtctcc
     4261 ccgatcaaca actgcgttgt cgatggcacg ctcaaaccaa tggccttcgc gagcgtatca
     4321 gcgtcccgtc caactgcatc ccgattgagc acggggtgag cacagcccgc atctcccttg
     4381 aacacgaacg acgtcagttg ttgaatttga gccgtggtca gttcaaacgc gccattcgct
     4441 accatccgcc gtttgagctc gtcggcgacg gtatccacca cgaaaatttc cttctctgcg
     4501 atgcaaagga gattgcagtc gaacgtagcc ccggcaataa tgctcctggc cgctttctcg
     4561 atgtccgccg tttcgtcgac gactaccggc ggatttcccg ggccggccgc aatgactttc
     4621 tttcccgcgc ccatggccgc tttcacaact ccgggcccgc cagtaaccag gatgagcttg
     4681 atcgcggggt ggtgaaacag cttgttcgcg ccctcgatgg tcggttcggc aacggtcgtg
     4741 atgatattgg caggcccacc ggccgccaca acgtactcgt taatcacctg gagtgcgtgc
     4801 gcgaataccc gcttccccgc cgggtgacca ttgaagacca caccattccc cgccacgaga
     4861 ttgcaaatcg tgttgttgat catcgtcgga acagggtgcg tggaaggagt caccgccgcg
     4921 ataaccccgt acggtgccat ttcctcgata gtgagcccgt gatcacctga ccgggctgtc
     4981 gtaacgaggt cctcgaaccc cggtgtctgg aggctcgcga ggcggtgttt ggcgatcttg
     5041 tccgccacgc gtcccattcc tgtttccgcg accgtttcgc gcgaaaacat ctcaccgtgt
     5101 tcgatcccga cccgtcggac agcctccacc acttccttcc gtttggcaac cggcatcgcg
     5161 atccattcac gctgcgcctt ttccgttgcc gcgaccgcct cgtcaatatc ggcaaaaacg
     5221 ccgcgtccga gagttgtcgc cggaggattc actgcccctg aatcaggggc gttgacggga
     5281 gttttcgccc cggtcgcgcc cgccggcgtg ccgagttgtt tcactacctg ctccacaatc
     5341 gcgcgaacgc gtgcttcgtc tatttgcacc gtacgcctcc atctgtgatc agcgtaagcc
     5401 gaacaagtat cccgatatgg ctactgcccc gatttccggt acacgatctt cccgtgtatg
     5461 tcgtactggt ctacgattgc cattatcacg gtgtcaacag ggcgtccgtc agaggacggt
     5521 gtaagccggg ccgaactccc gctcgcgcac agcactactt ccccgaggcc cgccccaacc
     5581 gcgtcaatcg ctacggtgta cgccttcttt ccttcccccg tcagcgggtc gagggtctcc
     5641 accaccagga gtttcgcact ccggagcttt tcatctttct gcgtggcaac aacggtgccc
     5701 gcgacacgtc cgagaatcat cgaatcattc ccccgaatcc ctcacgccac acccgccagg
     5761 attcatctgg caatagagcc accacgcact tccacctgtc gccgcgcggg gtacaaaaac
     5821 tggtttccct ccccccaaaa taacggtgtc cgggaaggcg gggcaagatg gtgctcgcgc
     5881 gcggctagcc cctgcaccta agcaggtttc atttgccaac agccagcgaa tcaaacccgc
     5941 agggcagggc ggcgatcgcg ggaagaagct cccggtacca ggttccgttg aatggaggac
     6001 ttcccagaag gtagtgatta ccgcctttaa tgccctcagt ctcccactca atgaggagca
     6061 gcctttgctg ccccctgctt gcggggattg cagtcagcac cgtgttggcg ttccgctcgg
     6121 gcgtaccgcc gccctcccag atcgtctcgc ccgttgccgc gtcccagatg cggaacgtgc
     6181 cctggacacg ctcaaggctg tcatttccga gtacgacacg acaacgccaa tcctccggct
     6241 cgtctaccat gacgcacacc ggttgctgga ctcggcgcag gtagaaatac gccagtttcg
     6301 ggcagtagaa ataatccatc accgcgtccg agaactgggg ccaaccgtcg atcaggttcc
     6361 accagagcac acctgttttc cgccatttcc tcaggcgcgt catctccacg aagtatttct
     6421 tcgcttccgc ctgcactatc tgcgacaaca ctacgaaatc atccagagtc tccggtgtcc
     6481 ggccgaacac ttctgcaacc tgtctgctca tcaacgcgat tctgtacgca taggctccgg
     6541 ggctcggggt agtatcggcc gcatggatgc gccattcctc attcccttct ggtggccaca
     6601 gcgcttcggg agacaggaac ttctccagcg aggattggct cgggcaccca tggtacccga
     6661 tttcacttac aaaatgagcc ctgttttcgg cgtaaaaacg gctcttgtag taatcacgcg
     6721 ggccccagag atgccgctca ggcattacct caacggtccc gtgttcacgc gattcagcgg
     6781 agtagtaagg agaactcgga atacagggtc gccacggatc gagccttttc a
//
LOCUS       DAOTNG010000168         6823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9664_length_6823_cov_5.436761, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000168 DAOTNG010000000
VERSION     DAOTNG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9664_length_6823_cov_5.436761"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="P5179_08945"
     CDS             complement(<1..545)
                     /locus_tag="P5179_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95382.1"
                     /translation="MPIELWDRAQWQEFSAARPSPFFAQTQWFEPTCRLMNAEPMYLA
                     LMSRSNPAFCVSLARKKRLGQTLLLTPAIAPYSAWIENFPDDLAPERREARRLEAMGE
                     FAAWCEQHAAYCRIVLPPQVVDVRPFLWRGWRTEVRYTYKIPLEHGVHQIFRQNVRRN
                     LRDAREANLRTECLTGEHAHGA"
     gene            752..1549
                     /locus_tag="P5179_08950"
     CDS             752..1549
                     /locus_tag="P5179_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95383.1"
                     /translation="MVRVKDKLWIFAVEQACDDTYYNIPITRVTPVEGAYYLGVERLA
                     MIVSREGPYPPLEPYLRIMRPLKEVVWSIVDSGGVTGWAQGKELDMLCDLACRFPNIT
                     GIFMDDFFNGKPEDGYAVHSIEKLTRIREKLVLPHRTLDLWAVLYRHQLDLPVIEHLN
                     MCDVVSFWEWWGRELPHLETNLEKLERLVPEKRRSLGIYLWDYGDRAPMPLDLMQHQC
                     RLGLEWLKSGRIDSMMFLTNYLLDQDFEAVEWTREWIAEVGDEVLAS"
     gene            1583..2269
                     /locus_tag="P5179_08955"
     CDS             1583..2269
                     /locus_tag="P5179_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRS95384.1"
                     /translation="MAKGQLTPELIRKLESVDSPTISNAIERFKVRSRREGYMGPQIV
                     CRFPHMGRIVGYATTCTIVEYDESFPPDPRERFKWLESIHNSPKPAICVVKDTCHRKG
                     WSSHWGEIVGTQVQVLGARAIITDGAVRDLEALEAMGMKTWSEYVVVSHGWIDVGKAD
                     IPVEVGGLVVNPGDILHADMNGVVSIPAEVLDDMPAAIDDVLNKEQTFIKSMRENGYD
                     IEAHRRQLEH"
     gene            complement(2517..4715)
                     /locus_tag="P5179_08960"
     CDS             complement(2517..4715)
                     /locus_tag="P5179_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS95385.1"
                     /translation="MLRSGVIVLLLSVRFVGAAEIAGKVTNEQGAPLPYAPVQVRQEE
                     SSSPASGVNADSTGVFVIRNVPPGRYLISADYVGHDPWREIIELREPLRTVEIVLRTR
                     PIRGQDIVVTAVRRLEATTAASVSVSLVTDDELEYRGVRSLDQALETLDGLQVFRSHS
                     VATNSVSIRGSSDVLGGGVGNRVLLLVDGRPALNTDSGGQSWSLVPIGAVERVEVVKG
                     AFSALYGSSAMGGVINLITRDPLGTPETRLVARYGAFEKPPAWMQYRSGLADFAETSV
                     LHADHRGSTGWLVYAAHNRSDGHREASDYSVWQAYGKVAHTVFPGLEIGGSVGGGRSH
                     AGYPHRWKSLLEPLKVPDEKKQDRQNKGWWNLDLTTKAGTLEHGLWNAGLYTYGMESA
                     TQQPVAGLSSSVTSQRMGSRLQWQKQLAGFLTQTIGGEGSLDFVRSDSVLYGNRSARN
                     AALFSLSEVLFATGQRLDFGIRGDYTSLPGKATETLVNPKLGLTVPLGKTFFVRASVG
                     RAFRSPSIAERYLVLEPAGGTEFAPNDSLRPEKMKSYELGLTHDLSPVLKMDVAAFVN
                     DYDDWIYWRELPVQTDGRYRFQVANLLKVRMSGVDAQATLEPFQGFSLALNYLYLRAE
                     DRTPGRSNPTIPYRPRHTFSGVVVFTHGRLRNTVVVRGRSRVEEEVFAAYQVDAPRGF
                     VLTNLRTDYSLRRWLTFSFEANNLFNVQYEEMARFRMPGRSFSVGLAMKR"
     gene            complement(4762..5454)
                     /locus_tag="P5179_08965"
     CDS             complement(4762..5454)
                     /locus_tag="P5179_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95386.1"
                     /translation="MQVSLFVAWDTTKGLATEAAGGPPSFASAYIPPGTNEYVIENIT
                     FGTYAALAASWRDTTMTGAAATTTIGAYGADPQAGDNTPLSITLSGSQPDLEIDIPVD
                     YSRAKVSTGPTGSISGTLTLSGTWPTKAVYLVVMPANKIGPPPAFAAMGQPVTMVALP
                     QFSGSKAYTIPNVPYGQGYAVAAYTFTPPMTLGFIGAYGVDLPADATLDSVTVGNGGA
                     IDLRNINFTIRP"
     gene            complement(5661..5975)
                     /locus_tag="P5179_08970"
     CDS             complement(5661..5975)
                     /locus_tag="P5179_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95387.1"
                     /translation="MSFTETLYHIFPALGILGLIALWILAMLCGMSYFSAKMPDAPSP
                     TRWKPAVVRFGMVAFMALVGILVAQRNGVWPDYRLAVGLAGAVAGLLVDLLWRVFTSR
                     MT"
     gene            6095..>6823
                     /locus_tag="P5179_08975"
     CDS             6095..>6823
                     /locus_tag="P5179_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95388.1"
                     /translation="MPESPRVAAIITTYFPNSHADVIVTRFLEGFPSDEGVIPPQARI
                     VSMWLDQIAPNDIGLAKAKEHGVPVFQSIRAALTLGGSDLAVDGVLLVGEHGDYPRDE
                     WDRQMYPRRALFEQICGVFAETKKSVPVFVDKHLSYNWPDAAWMLERARQLNVPLMAG
                     SSLPVGWREPELELDLDCDLTDALALGYGPLEAYGYHTVETLQCMVERRRGGETGVKA
                     VTCLRGDDVWKAGENGVWSQDLFEA"
BASE COUNT         1445 a         1976 c         2000 g         1402 t
ORIGIN      
        1 gctccgtgcg catgttcgcc ggtgagacac tccgttctga gattcgcctc gcgtgcgtcg
       61 cgaaggtttc tcctcacatt ctgtcgaaaa atttggtgca cgccgtgctc aagcgggatc
      121 ttatacgtgt acctcacttc tgtgcgccag ccccgccaca ggaatggtcg gacgtcgacg
      181 acctgtggcg gcaaaacgat cctgcagtat gcggcgtgtt gttcgcacca cgcggcaaac
      241 tcccccatcg cttccaaccg ccgggcttct cgccgctccg gtgcgaggtc gtcggggaaa
      301 ttttcgatcc atgcggaata cggcgcaatt gccggggtca gcaaaagggt ctgccccaac
      361 cgtttcttcc ttgccagaga gacacagaaa gccgggttgc tcctcgacat tagcgcgaga
      421 tacatgggtt cggcattcat cagccggcac gtgggctcga accactgggt ctgggcgaaa
      481 aaaggcgatg ggcgggccgc agaaaactcc tgccactgcg cgcgatccca caactcaatc
      541 ggcattgagt cctccatggc aacgatcggg cagacgctgg acacgctcct ttgtagttta
      601 cggcgcgagg tctcattgtg tccatgcctc gcggctttcg cgttggcttg cccctttctt
      661 cagagggcga gtaccatttc ccacaagtcc gtctgccatg ttctttccgg agaacaggcg
      721 cgttgccgaa cacacgcgag ggagtgtcag agtggtacgg gtgaaggaca aactctggat
      781 atttgccgtc gagcaggcat gtgacgacac ctattacaac atcccgatta cccgtgtgac
      841 cccggtggaa ggtgcgtact acctcggtgt cgagcgcctc gccatgatcg tatcacgcga
      901 aggcccttac ccgccccttg agccttacct ccgaataatg cgccccctga aggaggtcgt
      961 ctggtccatc gttgattccg gcggcgtcac cggatgggca caggggaagg aactcgacat
     1021 gctgtgcgac cttgcatgcc gtttcccgaa tatcaccggc attttcatgg atgacttctt
     1081 caacgggaag ccggaggacg ggtacgccgt ccattcgatc gagaaactca cgcggattcg
     1141 agaaaaactg gttctccctc accgaacgct ggatctctgg gcagtgctgt accgacacca
     1201 gctcgatcta cccgtgatcg aacacctgaa tatgtgcgac gtcgtgagtt tctgggagtg
     1261 gtggggacgg gaattgcctc acctggaaac gaatctggag aaacttgaac ggctcgttcc
     1321 tgaaaagcgg cgctcgctcg gcatctatct ctgggattac ggagatcggg cgccaatgcc
     1381 actcgacctt atgcaacatc agtgcaggct aggactcgag tggttgaaaa gtggaagaat
     1441 cgatagcatg atgttcctga cgaattacct gctcgaccag gattttgaag cggttgaatg
     1501 gaccagagaa tggattgccg aagtgggaga tgaggtgctc gcttcctgaa tatccccaac
     1561 gtccgttgaa aggagttgca taatggcaaa aggtcagctc acgccggaac tgatacgcaa
     1621 gctggaatcg gtggacagcc cgaccatttc caatgcgatt gagcggttca aggtacggtc
     1681 ccgtcgcgag ggatacatgg ggccccagat tgtgtgccgg ttcccccata tggggcgtat
     1741 cgtggggtat gccacgacct gcacgatagt cgagtacgac gagagcttcc ccccggatcc
     1801 cagggaacgg ttcaaatggc tggagtcgat tcacaactcc cccaagccgg ccatctgtgt
     1861 ggtgaaggac acatgtcacc gcaaaggatg gtcatctcac tggggcgaga tcgtaggcac
     1921 gcaggtacag gttcttggcg cgcgagcaat catcactgac ggcgcagtcc gggaccttga
     1981 agcccttgaa gcaatgggga tgaagacgtg gagcgaatat gtcgttgtct ctcacggctg
     2041 gattgacgtt ggcaaagctg acatcccggt ggaggttggc gggctcgtgg tcaatccggg
     2101 cgacattctc cacgccgata tgaacggtgt cgtcagcatt ccggcggagg tgcttgacga
     2161 catgccggcg gcaatagacg atgtgctcaa caaagagcag acgtttatca aaagcatgcg
     2221 ggagaatggg tacgatattg aggcgcaccg ccggcagttg gagcactgag tctcgaacaa
     2281 cctgcgccgc ggagaggcag aatcaggaca gcctctccgc ggtttgtttg tccggtggag
     2341 tcattcggac gattcaggtg tctttcaccc gcgcggcacg ttcaactgca cctccgcaca
     2401 tctccggaaa gaaatggcag taactgcatt tcgcggcacg ttcgacttcg taatctcttc
     2461 gtggcaaaag cctgttcaac gatggcccga actgaaaccc gtcaaagaac ggagcgctac
     2521 cgtttcatcg ccagaccgac cgagaaggag cgtcccggca ttctgaagcg agccatctcc
     2581 tcatactgca cgttgaacag gttgtttgct tcgaacgaaa aggtcagcca gcggcgcagt
     2641 gagtagtcgg ttcgcaggtt tgtcagcaca aagccgcgcg gagcatcaac ctggtatgcc
     2701 gcgaagacct cttcttccac acggctcctt cctcgaacaa ccaccgtgtt tcgcagcctt
     2761 ccgtgggtga acaccacgac acccgagaac gtgtggcgtg gacggtacgg aatcgtgggg
     2821 ttgcttcgtc ccggcgttcg gtcttcggcc cgcaaataca ggtaattgag tgcaagcgag
     2881 aacccttgaa acggttcgag cgtcgcctgc gcatccaccc cgctcattcg cactttcagt
     2941 agattggcca cctgaaatcg gtagcgcccg tcggtctgca cgggcagttc acgccagtag
     3001 atccagtcgt cgtaatcgtt cacgaacgca gccacgtcca ttttcagcac cggcgagagg
     3061 tcgtgagtca aaccgagttc gtatgatttc atcttttccg ggcgcaacga atcgttgggc
     3121 gcgaattctg tccctcctgc cggttccaac acgagatatc tctcagcgat actgggagat
     3181 cgaaaggctc ttccgacgga ggcccgaaca aagaacgttt tcccaagcgg gaccgtaagg
     3241 cccagcttcg gattgaccaa tgtttcggtg gcctttcccg gaagcgaggt gtaatcgccc
     3301 cgaatgccga aatcgagccg ttgtccggtg gcaaaaagaa cttcggagag ggagaaaaga
     3361 gccgcgttgc gagccgatcg attcccgtag agaacgctgt ctgagcgtac gaagtccagt
     3421 gaaccttctc ccccgattgt ctgggtaaga aaacccgcca actgcttttg ccactgcagt
     3481 cggctgccca ttctttgtga cgtgacggaa ctgctcaacc cggcaaccgg ttgctgtgtg
     3541 gcggactcca tcccgtaggt gtacaggccg gcgttccaca gcccgtgttc gagagtgccg
     3601 gcttttgttg tcaagtccag attccaccac cctttgttct gccggtcctg tttcttctca
     3661 tcagggactt tcagcggctc cagcaggctt ttccatcgat ggggatatcc ggcgtgagac
     3721 ctgccgccgc ccacgcttcc tccgatctcg agaccgggaa agaccgtgtg cgcgaccttc
     3781 ccgtaggcct gccagacgga gtaatccgac gcttcgcggt gaccgtccga cctgttgtgg
     3841 gcggcataca ccaaccatcc ggtcgaacct ctgtgatcgg catggagcac cgacgtttcg
     3901 gcgaaatccg cgaggcctga ccggtattgc atccaggcgg gtggcttctc gaaggcaccg
     3961 taacgagcga cgagtctcgt ctccggtgtt cccaacggat cgcgggttat gaggtttatt
     4021 accccgccca ttgcagacga accgtagagc gcggagaacg ctcctttcac aacctccacc
     4081 cgctccacgg caccgattgg aacgagactc catgattgcc caccggaatc ggtgttaagt
     4141 gccgggcggc cgtcgacgag cagaaggact ctgttcccca cgcccccacc cagcacgtcc
     4201 gaagagccgc ggatactgac cgaattcgtt gcaacactgt gggaccgaaa tacctgcaac
     4261 ccgtccagcg tttccagcgc ctggtcgagt gagcgaacgc cgcgatactc caactcgtcg
     4321 tccgttacca gcgatacgga taccgacgca gcggtcgttg cctccaggcg gcgaactgcc
     4381 gtcaccacga tatcctgccc gcgaatcggc ctggttcgga ggacgatttc caccgttcgc
     4441 agcggttccc ggagttcgat aatctcacgc cacggatcgt ggcccacgta atccgccgag
     4501 atgaggtacc tgcccggggg cacatttcgg atcacgaaca cgccggttga gtcggcattg
     4561 acaccggacg ccggcgatga cgactcctcc tggcgcacct gcaccggagc gtacggcaac
     4621 ggggcaccct gctcgtttgt tacctttccg gcgatctccg ctgcgcctac aaagcggacg
     4681 gagagtaata agacgattac tcccgagcgc agcatcaagc gaccacgtac cgagttcgtg
     4741 aaccgaacca tggcaggaat gtcagggacg gatggtaaaa ttgatgttac gcagatctat
     4801 cgcaccgcca ttacccacgg taaccgagtc gagtgttgca tccgcgggca gatccacacc
     4861 atacgcgccg atgaacccca gcgtcatcgg gggggtgaac gtgtaggcgg cgaccgcata
     4921 cccctgtccg taaggtacgt tagggattgt gtaggctttg gagccggaaa attgcgggag
     4981 cgcgaccatg gtgaccggtt gacccatcgc ggcaaatgcg gggggaggac cgatcttgtt
     5041 tgcgggcatc acaacgagat atacggcctt tgtgggccat gttccagaga gtgtgagagt
     5101 tccggaaatg ctgccggtag gtccggttga gactttggcg cgcgaataat cgaccgggat
     5161 gtctatttcc agatccggct ggcttccact cagagtgatg gagagcggcg tattgtcacc
     5221 agcctgtggg tcagctccgt acgcacctat tgtggttgtc gcggccgctc ccgtcatggt
     5281 ggtgtcgcgc caggaggctg cgagcgcggc gtaggtgccg aacgtgatgt tctcaattac
     5341 gtattcattc gttccgggag ggatgtacgc actggcgaac gacgggggcc ctcctgcggc
     5401 ttcagtcgcg agccctttcg tcgtatccca cgcgacaaac agggaaacct gcacggttcc
     5461 cgtgtccgga cgtgcttttg caccggggat tattctcccg tgtattcgac cggtttgggg
     5521 tggattcgta ctatcgcagc ccgtgaacgc cgccaatccg gccaacaaac accacgctaa
     5581 agaacaacgc ttcatcgttc cgatctccag ttgcgatttg gctatctccg cgggaccatt
     5641 ccgtaccgca cacatctcat tcacgtcatg cgcgacgtga agacccgcca caacaagtcc
     5701 accagaagtc ctgccactgc ccctgcaaga ccgactgcaa gacggtagtc cggccatacg
     5761 ccgttgcgct gggcaactag gattcctacg agcgccatga acgcgaccat cccaaatcga
     5821 acgacggctg gcttccatcg cgttggcgac ggtgcgtcgg gcattttcgc ggagaaatat
     5881 gacatgccac acagcatcgc gagaatccac agcgcgatga gccccagaat accgagcgcg
     5941 gggaatatgt gatacagcgt ttccgtaaaa ctcatcattt cctccaatag aaacgtaccg
     6001 cgtgcagacg gaaaacacca ctcccgcggt ggtaccgact tgcgccgggg gcaattgtcc
     6061 ccctattcta catgtcgcta aaggaggttc tgccatgccg gaaagcccga gagtcgccgc
     6121 gatcattact acctatttcc cgaactcaca cgcggatgtg attgtaaccc gtttcctcga
     6181 ggggttcccc tctgacgaag gagtaatacc gccgcaggcg agaattgtaa gcatgtggct
     6241 ggatcagatt gctccaaacg acatcggtct ggcgaaagca aaagagcatg gtgtgccggt
     6301 gttccagagc attcgagcag cgttgacgct cggtggaagc gatctcgccg tggatggcgt
     6361 gcttctcgtc ggggaacacg gtgactatcc gcgagacgaa tgggaccgtc agatgtaccc
     6421 gcgccgcgca ttgttcgagc agatctgcgg agtattcgca gaaacgaaga aatcagtccc
     6481 cgtgttcgtg gacaagcatt tgtcgtacaa ctggcccgac gccgcatgga tgctggaacg
     6541 ggcaaggcag ttgaatgtgc ctcttatggc gggatcatcg ctgccggtgg gatggcgcga
     6601 accggagttg gaactggatc tggactgcga tctcacggac gccctcgcgc tggggtacgg
     6661 gccgctggaa gcgtatggat accacacggt cgagacattg cagtgcatgg tggaacgccg
     6721 acgtggtgga gagaccggtg tgaaggcggt tacctgcctc cgcggcgatg acgtgtggaa
     6781 agccggagaa aacggtgtct ggtctcagga cctttttgaa gcc
//
LOCUS       DAOTNG010000169         6766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9758_length_6766_cov_5.377440, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000169 DAOTNG010000000
VERSION     DAOTNG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9758_length_6766_cov_5.377440"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1207
                     /locus_tag="P5179_08980"
     CDS             86..1207
                     /locus_tag="P5179_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRS95389.1"
                     /translation="MPDPRFDQLAQVLIGYSTALKEGENVLIEAAEAPDELVISVMRA
                     ARNVGARVFVNLFHPRVQREFVRLCSDEQMTLHGEIEKARMEKMQAYISIRGTSNISE
                     MSDVPREAMRIYERRFLQPVHFDVRVKQTKWVALRYPTPSMAQLAQMSTEAFEDFYFR
                     VCTLDYGRMAAAMVPLRELMEKTKLVRIVGPDTDLRFSIEGIPVIACTGQNNIPDGEI
                     FTAPVRDSVNGKIAFNARSPYHGINFESIRLEFDRGKVVRASANNTQKLNEILDADEG
                     SRYVGEFAIGVNPFIRRAMGDILFDEKIAGSIHLTPGNAYEEADNGNRSDIHWDMVLL
                     QDADSGGGKLYFDDVLVRQDGEFVLDSLRPLNPDELGKS"
     gene            1319..2446
                     /locus_tag="P5179_08985"
     CDS             1319..2446
                     /locus_tag="P5179_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS95390.1"
                     /translation="MEYRAYGNTGLKLSLLGFGCMRLPMKDEHVVEDEAIRMLRYAID
                     HGVNYIDTAFFYCNHESEVVVGKALKDGYREKVTLSTKNPVHEPDGKKWRQTLDEQLR
                     KLDVDKIDVYHFHGIGWDEWNEKFIVPGGPADEMRRAQKEGIVTHCAFSFHDKPENLI
                     KLVDTGFFEGVLLQYNLLDRANEAGIAHAHEKGLGVVVMGPVGGGRLAAPSDGIKKLV
                     PGPVQSSAEAALRFVMGNPGVTVALSGMSTMEQVKENLATAETAGVLSEADRAQIAKN
                     LDEVKALSDLYCTGCNYCMPCPHGIDIPRNFELMNYYRVWGLKDYAKERYARLKERKV
                     DDSIVEAWAAACTQCGECEPKCPQNIPIPEQMKEVAATLGT"
     gene            2528..3781
                     /locus_tag="P5179_08990"
     CDS             2528..3781
                     /locus_tag="P5179_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS95391.1"
                     /translation="MNLSSQVSSFFQNSLRAMKSRNYRLFFFGQGVSLIGTWIQRVAM
                     SWLVYRITNSAMLLGTVEFVGMLPTFLLAPFTGVLADRWNRHTIMLGTQIGFTLSALA
                     LGLLVVTEIVQVWHILVLSAISGIVMAFDVPARQSLVVDMVEDKRDIGNAIALNSTMF
                     NGARLIGPSIAGLIIAAFGEWICFFINAATFAAVIAALLAMKISPRTPNGAHRKVLVE
                     LKEGAVYAYRTVPIRATLILMAFVSVVGMSYMTLLPVFAKDVLRGGPDTLGFLMAITG
                     VGAVGGAFYLASRKDDLTINWQIPASLMLLGLGLLALGGSRTVWLSYAIVLVTGFAQM
                     VIIASSNTVIQTVVDEDKRGRVMSLYAMAFMGMAPFGSLYAGTSAKLFTAPVAVIASG
                     VLCVFGAALFAARLPSLRKMAAEPA"
     assembly_gap    3889..3988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4139..5755
                     /locus_tag="P5179_08995"
     CDS             4139..5755
                     /locus_tag="P5179_08995"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRS95392.1"
                     /translation="MGICRETIGWNATSATGVVAAGAAEAVAAGISLLEAGGNAADAA
                     VATIFALNVTDHGDCSIGGEVPLIIHEARTRQTKVLCGQGRAPRSEDAIAWYMRHGIP
                     DDIKMAPVPSVVDLCITTLQRYGTRSLSDVVAPALALLDAGSEEWQPKLAHTLRRMVE
                     EERITTGTREEKLQAACDRFYGRHASRNDIADELEAYWIERGGFLRRADLAAHRTTVE
                     EPVCTNYRGYTVCKCGPWTQGPFLCQTLRLLEGFDLRAMGRLSPDAIHVATEALKLAL
                     ADRDEYYGDPEFVRVPLAALLSDHYTAIRRTLIDMNKASLEVRPGDPDGMKPLKKGGL
                     FRPGVGGTTTCVVADRWGNVVSATPSANVPKTPRDGGKAGVTYGNRLRSLNTTPGHPN
                     CIAPGKRPRITLTPTLVLKDGKPVMAISVAGGDLQDQVTLNLLLNAIDYGMAPDAAVT
                     APRFATAHHQDSFDPDPDRQKTFKEAGSLRLNDRVSEATREELVRRGHSVHVASGPIG
                     HPVMLAINPETGLFHAAGDPAAGRHAAGLE"
     gene            complement(5763..>6766)
                     /locus_tag="P5179_09000"
     CDS             complement(5763..>6766)
                     /locus_tag="P5179_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95393.1"
                     /translation="ELVDTESQWRLGQVIYESFPVPEGARHDTGRVFRPEAFRRESVR
                     ACRIERQERTALWQRVLFSCDFPGCTNPGGLRVEIRLWHEREQLDFVYHLRKEYTTSP
                     EAVYVAFPFSGNGAGLRYDGQGGEVMPGAGQVEGSAADWQTIQHYVRVADGRGQTILS
                     SPRVPLVQLGGLNLGAWRYVPEIPRPHVFSWVMNNYWWTNFRASQGGEWSWTCSLTST
                     EESSRGTAARFGAECHSPLVARLERVAKSETRSRIRVTPGNVLAVEMRPERGGDGIIL
                     QLRELDGQDTTFRIEVPDGTSAPHMEEVDVTGRPLGTLPDHVPIPGFGVRFVRLALR"
BASE COUNT         1292 a         2030 c         2115 g         1229 t
ORIGIN      
        1 aatgaggggc gccctctccg cgttgcgtat cttcaggtac gctgcccgga cacatcacgc
       61 gaccctccac aacgagaggc tccccatgcc cgatccccgt ttcgatcagc tcgcacaggt
      121 tctgatcggc tattcgaccg ccctgaaaga gggcgaaaat gtcctcatcg aggccgcgga
      181 agcgcccgat gagctcgtga tctccgtgat gcgggccgcg cgcaacgtcg gtgcgcgggt
      241 gttcgtgaac ctgttccatc cccgggtgca gcgcgaattc gtgcgcctct gttcagacga
      301 gcaaatgact ctccacggcg aaatcgaaaa ggcgcgcatg gagaaaatgc aggcgtacat
      361 ttccatccgc ggaaccagta acataagcga gatgtccgac gtcccgcgcg aagcaatgcg
      421 catttatgag cgcaggttcc ttcaaccggt gcatttcgat gtgcgggtga aacagacgaa
      481 atgggtcgcc ctgcgctacc cgacgccatc catggctcag cttgcgcaga tgagcactga
      541 agcattcgag gatttctact tccgggtctg cacgctcgat tatggcagga tggcggccgc
      601 gatggtcccg ttgcgggaac tgatggagaa gacgaaactt gtccgaatcg tcggccccga
      661 cactgacctc cggttctcga tcgaaggcat tcccgtcatc gcctgcacgg ggcagaacaa
      721 catccccgac ggagagatct tcacggcgcc ggtaagggat tcggtcaacg ggaagatcgc
      781 cttcaacgcg cgcagcccgt accacggcat caatttcgaa agcatccggc tcgaattcga
      841 cagagggaag gttgtcaggg ccagcgccaa caacacgcag aaactgaacg agattctgga
      901 cgcagacgag ggttcgcggt acgtcggcga gttcgccatc ggcgtcaacc cgttcatccg
      961 gcgtgcgatg ggggatatcc tgtttgacga aaaaatcgcg ggttcgattc accttacccc
     1021 cggcaacgcg tacgaggagg cggacaacgg caaccgctcg gacatccact gggacatggt
     1081 gctgcttcag gacgccgaca gcggaggagg aaagctgtat ttcgatgacg ttcttgtccg
     1141 gcaggacggg gaattcgtgc tcgattccct caggccgctg aatcccgatg aactgggcaa
     1201 gagctgacgg gaggccgtcc gttctttggc cctgggtgtg tggccgacgt acattttgtt
     1261 catgtggcac tttcgccgga ttttgagccg ttgtcaacgg cgcgcaggag gatgattgat
     1321 ggagtatcga gcgtacggaa acacaggact caagctttcg ttgcttggtt tcgggtgcat
     1381 gcggctgccc atgaaagacg agcatgtcgt tgaggacgaa gccatccgga tgctccggta
     1441 tgcaatagac cacggggtca actacatcga cacggcgttt ttttactgca accacgaaag
     1501 cgaggttgtg gtaggcaagg cgctgaagga cggttaccgc gagaaggtga cgctttcgac
     1561 aaagaacccc gtgcatgaac cggacggcaa gaagtggcgc cagacgctgg atgagcaact
     1621 ccgcaagctc gatgtggaca agatcgacgt ctaccacttc cacggcatcg gctgggacga
     1681 gtggaacgag aagttcatcg ttccgggtgg gcctgcggac gaaatgcggc gtgcgcagaa
     1741 ggaagggatc gttacccact gcgcgttttc gtttcacgac aagcccgaga acctcatcaa
     1801 gcttgtggat acgggcttct ttgagggcgt gttgctccag tacaacctgc tcgaccgcgc
     1861 aaatgaggct ggcattgccc acgcccatga aaaagggctc ggcgtggtgg tgatgggacc
     1921 cgttggcggg gggaggctcg cagccccttc ggatggcatc aagaagctcg ttccggggcc
     1981 cgtgcagagc tccgcggaag ccgcgctccg gtttgtcatg ggcaaccccg gcgttacagt
     2041 ggcgctttcc ggcatgagca ccatggagca ggtgaaagag aaccttgcaa ccgcggaaac
     2101 tgccggagtg ctttcggagg ccgaccgggc acagatcgcg aagaacctgg atgaagtgaa
     2161 ggcactgtcc gacctgtact gcaccggctg caactactgc atgccgtgtc cccacggaat
     2221 cgacatcccg cgcaacttcg agctcatgaa ctattaccgc gtgtgggggc tcaaagatta
     2281 cgcgaaggag cgttacgccc gtctgaaaga gcgcaaagta gacgacagca ttgtcgaagc
     2341 gtgggctgcc gcctgcacgc aatgcggcga gtgcgaaccc aagtgcccgc agaacattcc
     2401 catcccggaa caaatgaagg aagttgccgc aacactcggc acctgacccc gctcttcagg
     2461 caccgcgagg aagggggcgc aacctcaaga cgcccccttt cctttccctg cgagggtaac
     2521 ccggaacgtg aatctttcct ctcaggtctc ctcctttttc cagaacagcc tccgagcgat
     2581 gaaaagccga aattaccggc ttttcttctt cgggcagggc gtatcgctca tcggcacgtg
     2641 gatccagcgc gtcgccatga gctggctggt gtaccgaatc accaactccg ccatgttgct
     2701 cggaacggtg gagttcgtcg gcatgctgcc gacgttcctc cttgctccgt tcaccggtgt
     2761 tttggccgac cggtggaacc ggcacaccat tatgctggga acgcagatcg gcttcacgct
     2821 gagtgcgctc gcgctcgggc ttctggtggt gacggagatc gttcaggtgt ggcacatcct
     2881 cgtgttgagc gcgatctccg gaatcgtgat ggcgttcgac gtgccggccc gccagtcgct
     2941 tgtggtagac atggtggagg acaaaaggga catcgggaat gcgatcgcgc tgaactccac
     3001 gatgttcaac ggcgcacgcc tcatcgggcc ctccatcgct ggtctcatca tcgccgcgtt
     3061 cggtgagtgg atctgctttt ttatcaacgc agccactttc gctgcggtca tcgccgccct
     3121 gcttgcaatg aagatttctc cccgaacccc gaacggggcg caccggaaag tgctggtgga
     3181 gctgaaggag ggagcagtgt acgcgtaccg gacggttcct atccgcgcaa cgctgatcct
     3241 gatggccttc gtaagcgtcg tcgggatgtc ctacatgact cttctccccg tgttcgcaaa
     3301 ggacgtgttg cggggcggac ccgatacgct cggtttcctt atggcgatta ccggcgtggg
     3361 agccgtgggc ggggcattct acctcgcctc ccggaaagac gatctcacca tcaactggca
     3421 gataccggcc tctctgatgc tgcttggctt agggttgctc gcgctggggg gctcccgaac
     3481 ggtgtggctt tcctacgcca ttgttctggt tacgggcttc gcgcagatgg tgatcatcgc
     3541 gtcgagcaac acggtcatcc agacggtcgt ggacgaagac aaacgcgggc gggttatgag
     3601 cctgtacgcc atggcgttca tgggaatggc gccattcgga agcctttacg cgggtacttc
     3661 ggcgaagctg ttcaccgcgc ctgttgcggt gattgccagc ggggtcctct gcgttttcgg
     3721 cgcggcactt tttgcggctc gccttccctc cctgcgaaag atggctgccg agcccgcgtg
     3781 acaggccggg gcatcgcgcc gggcctccgc cgggaagtcc ctccaacgcg ccatggtaag
     3841 gcctgaaggg tcggcatacc acagcccacg ggcaacgccc cgggcgcgnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gttccgcccc gttggggctg ctgccggttt
     4021 ctcgaacttt ttttcttcct cctcttgaca cgaagacggc tcttcaccac agctagatac
     4081 gggtgtgttt gcctcgtttc cattccaacg ccaaccagaa ccgggggtaa tgcacagaat
     4141 gggtatctgc agggaaacaa tcggctggaa cgccaccagc gccacagggg tggtggccgc
     4201 gggcgcggcg gaggcagttg ccgccgggat cagcctcctc gaggcgggag gaaacgccgc
     4261 tgatgcagcc gtcgccacga ttttcgcgct gaacgttact gaccacggcg attgctccat
     4321 cggcggggag gtgccgctca tcatccacga agcacgcacc cggcagacga aggtgctgtg
     4381 cgggcagggg cgcgccccgc gttcggagga cgccatcgcg tggtacatgc ggcacggcat
     4441 ccctgacgac atcaaaatgg cgcccgtgcc gtcggtggtg gatctctgta tcaccacgct
     4501 gcagcggtac ggcacgaggt cgctttccga cgtggttgcg ccggcgctgg cgctgctcga
     4561 cgccgggtcg gaggagtggc aaccgaagct cgcgcatacg cttcgcagaa tggtggagga
     4621 ggagcgcatc accacgggaa cgagggaaga aaagttgcag gcagcgtgcg accgtttcta
     4681 cgggcggcac gcgagccgga atgacattgc ggacgagctt gaggcctact ggatcgagcg
     4741 gggtgggttt cttcggaggg ccgatctggc cgcgcaccgg accacggtcg aggagcctgt
     4801 ctgcacgaac taccgggggt acaccgtctg caaatgcggg ccgtggactc agggaccgtt
     4861 cctgtgccag acgttgcgtc tgctggaagg attcgacctg cgcgcgatgg ggcggctctc
     4921 acccgatgcg atccacgtgg caacggaggc gctcaagctg gcgctggcgg accgtgacga
     4981 atattacggc gacccagaat tcgtgcgcgt gccgcttgcc gccctcctgt cggaccacta
     5041 caccgcgatt cgccgcacgc tcatcgacat gaacaaggca tccctcgaag tgcggccggg
     5101 cgacccggac gggatgaagc ccctgaaaaa gggcgggctg ttccgccccg gggtgggagg
     5161 aaccactacc tgcgtggtgg cggatcgctg gggaaacgtc gtatcagcca cgccaagcgc
     5221 gaatgtgcca aaaacgccac gggatggcgg caaagccggg gtgacctatg gcaaccggct
     5281 gcgcagcctt aacaccacgc cgggccatcc caactgcatt gcgcccggca aacgcccgcg
     5341 gatcaccctc acgccgacgc ttgtcctgaa agacgggaag ccggtcatgg cgatcagcgt
     5401 tgccgggggc gatctgcagg atcaggtgac gctgaatctc ctgttgaacg ccattgacta
     5461 cgggatggcg ccggatgcgg ccgttaccgc gccgcgtttc gccactgcgc accatcagga
     5521 ttcattcgac cccgaccccg accggcagaa gacgttcaaa gaggcggggt cgctccgcct
     5581 caacgaccgg gtgagcgaag ccacgaggga ggaacttgtc cgccgcgggc attcggtgca
     5641 cgtcgcctcc gggccgatag gtcatccggt gatgctggcg atcaacccgg aaacgggtct
     5701 tttccatgcg gcgggagacc cggcggcggg gcgtcatgcc gcaggactgg aataggtcgc
     5761 gttcaacgca acgcgagacg cacgaatctg acgccgaaac cgggaatcgg cacgtggtca
     5821 ggcagcgtgc cgaggggccg cccggtgacg tccacctctt ccatgtgtgg tgcggaagta
     5881 ccgtccggca cctcgatccg gaacgtggta tcctgcccgt caagttcgcg gagctggagg
     5941 attatcccgt ctcctccgcg ttccggacgc atttccactg ccaacacgtt gccgggcgtt
     6001 acacggatcc gcgagcgggt ttcggatttt gcgacccgtt ccagacgtgc gaccagcgga
     6061 ctgtgacatt ccgctccgaa tcgtgccgcc gtcccgcggg aggattcctc cgtcgaggta
     6121 agggagcagg tccacgacca ttcgccgccc tgcgaggcgc ggaaattcgt ccaccagtag
     6181 ttgttcatca cccaggagaa cacgtgcggg cgggggattt ccggcacata ccgccatgcg
     6241 ccgaggttca gcccgccaag ctgaacgagc ggcacacggg ggctcgaaag gatggtctgc
     6301 ccccggccgt cggcgactcg gacatagtgc tggatcgtct gccagtcggc ggcagagccc
     6361 tccacctggc cggcacccgg catcacctcc ccgccctggc catcgtagcg cagtcctgcc
     6421 ccattgccgg aaaacgggaa cgccacgtac accgcttccg gagaagtggt gtactccttc
     6481 cgcaggtggt acacgaaatc aagctgttcg cgctcatgcc agagacggat ttccacacgc
     6541 aggcccccgg gattggtgca tccggggaaa tcgcaggaga agaggacgcg ttgccagagc
     6601 gcggtgcgtt cctgccgttc aatgcggcag gcgcgcacgg actcccgtcg gaacgcctcg
     6661 ggccggaaca cccgcccggt atcgtgacgc gcgccttcag gcaccggaaa actctcgtag
     6721 atcacctgcc ccagccgcca ctgcgattca gtatccacca gttcgc
//
LOCUS       DAOTNG010000170         6740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9805_length_6740_cov_5.439791, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000170 DAOTNG010000000
VERSION     DAOTNG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9805_length_6740_cov_5.439791"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="ruvB"
                     /locus_tag="P5179_09005"
     CDS             <1..874
                     /gene="ruvB"
                     /locus_tag="P5179_09005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRS95394.1"
                     /translation="ALDHVLFHGPPGLGKTTLAHVIGNELGANVRVTSGPILERPADL
                     VGLLTKLEHGDVLFIDEIHRIDRVVEEYLYSAMEDFAIDIMTGTGPGSASVRVNLEHF
                     TMIGATTRTGLLTAPMRDRFSIIERLNYYTAEELAHIVRRSARILNTPIDEGGSNEIA
                     RRSRGTPRIANRLLRRARDFAQVRGDGTVSQEIATHTLKLLDVDELGLDDMDKRILLT
                     IMDKFGGGPVGLNSIAASLNEEPDTIEEVYEPFLIQEGLLHRTPRGRVVTVSACRHFG
                     RAGLAQGGAQSALF"
     gene            979..2025
                     /locus_tag="P5179_09010"
     CDS             979..2025
                     /locus_tag="P5179_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95395.1"
                     /translation="MNRQIVCLIVTLGVFFQPVSAGSMLETYHLSTRGKLASFSAIPA
                     PVASLQNESSPQVTSEEPVVTGKSPVKAFFLSLLLPGLGEWYTGHRIRSAAFLAVEGT
                     AWGLWSNFRGNGKDWENRYIAFQAAHWSFPRYDAYRHAVWDRVGQLTNFWDPSRPLSA
                     RQQDSLSVLVGAHHYDDCCGQPMPSEDDRLEMIGKYYAFSYGWDDATVYNNPNQLLRD
                     VFPAPAPGDNGVWGLNRYDWLTVLGGALVTPAYDTSSYVFQVPNLNLVQSANRDEYMS
                     MRQKSNSNYSNAEKMVTVILFNHIISAIHAARLAKSGNVATGEMPRVNTSLRMALQRT
                     DRDMVPMVMLWRRF"
     gene            2064..2666
                     /locus_tag="P5179_09015"
     CDS             2064..2666
                     /locus_tag="P5179_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95396.1"
                     /translation="MTVACVAPAQAVSPRGAFFRSLLLPGWGQLATDAPKRAAVFAAA
                     EGALLAGAAGTAIMGNIYRDDYRALAAAVAGANLAGKGDGYFSDLAFYETRAHHNQAA
                     LIYDQPDPTLYAPEDDWQWPSTEDRMTYRRYWNRSRAMGQRLDYVLFAVSLNHLASAV
                     DAAKQASRRNPGTSAWQNVVPRVGTAAASGRVDLRCAWSF"
     gene            2697..5777
                     /locus_tag="P5179_09020"
     CDS             2697..5777
                     /locus_tag="P5179_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRS95397.1"
                     /translation="MGVGRGAGGRVIAGWIAMIWGVGLLATPASAQWWNEISHFGRNK
                     VNYQAFEWRVIETENFRVYYYQGEGDIARVASQMAEDAYARISRTLDYRLSQPVPLLV
                     YDSHNDFEQTNATWGLIDESTLGFTEAFKNRVVVPFTGSYTQFRHVIHHELVHAVMFD
                     MFYRRQGGLSTASQLWWGPPLWFAEGLAEYLSTDWTTEKNMWVRGATIDGYLRLDGYQ
                     AYTAGYSLLRWIADEFGADKIPAITRRMAITKEPAASFRAVLGVSLDDLQTRWERHLK
                     RFYWPEIERRELTDEMGRRLTDHRKDRNHYNFAPAFSPHGDRIAFLSDRDDYASIYIM
                     SAIDGRILRKVVTGERSSHFEEMHWSRGSLSWSPGGSHLVFAAKHHDLDALYIVSAEN
                     GRIQRMFRWRQFNALFSPSWSPDSLNIVFVGVREGRSDLYLVNTLTGELRQLTDDFYD
                     EIAPRWSPDGTRIAFASDRVLDGEDSNVLPVGFDRNVFVMDVSTGVITQITRAEGEDS
                     SPCWSPDGRKLAFTSDRNGIYNLYVADLDSGTERPLTNVIDGVFQPDWSRDGRKIAVA
                     AFNVAGYDIFTLTDPLAKWQADDPLPPAEFLERRREEAQSDSSRYLERPPVSGLGHWR
                     FSLQPHDSSGNPLTFVSKPYDVHFSPDLVSGLVMFDNINGLMAQTMLRVSDTMGDHQI
                     QIYTHIYRNVEDADFQFAYYNLRKRPNWGFSLFQFHQYYLQSFAATEWTSDRVLGGAV
                     LLNYPFNKFTRLETSLELSDRERANFSYDTYLRTPVRRLPGVRTLLPSFALINDNTLW
                     GVTGPTNGSRSAFQVKFAPEIGFNTYAYTTLIGDYRKYLRVSADYALALRVSSGMSVG
                     RNPGRFLLGGTSYWINFHYTPTYSVTNDSLIPITQFITPLRGSRFGELSGSRYALANI
                     EFRYPLVRELAWGWPLPIVLRNIRGILFLDLGTVWGDGRGFAPFAGNGIKLNYTSAGM
                     TNAPTRGPLAVGGFGFGWRMNLGVAVLRWDIAWPTDLDRTLGGSRQYWSLGADF"
     gene            5874..>6740
                     /locus_tag="P5179_09025"
     CDS             5874..>6740
                     /locus_tag="P5179_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95398.1"
                     /translation="MRVRTSAYVAVLCAAGFASAGAAAPIPCGSHLFTALPESFFRED
                     VSRAKPARNPVVGAKETLWIQNFAAGQLEEVSVTCRAVNRRSVILVADACWSALPSAT
                     AIDPADVTRLSAAFEENAGSPAPVGKGIYDVLATAFAPVNPVGGDSLVYIALTEIKSN
                     YTGGYIVGYVSPNDRVDPSLYDRSNFRNLVVLDGLSRTTAVQRERTLAHEFLHVIHMG
                     IDRYEERWIDEGIAVLAQVLCGYQGNTGREFFANTGIGLFNDGTATPSLGDYDKSYMI
                     MQYLADQFGVQTI"
BASE COUNT         1345 a         1965 c         2024 g         1406 t
ORIGIN      
        1 cgctctcgac cacgttcttt tccacgggcc gccaggtctg gggaaaacga cgctggcgca
       61 cgtgataggg aacgaactgg gcgctaatgt tcgtgtcacc tccggcccca ttctggaacg
      121 tcctgccgat ctcgtgggcc ttctgaccaa actggaacac ggggatgttc tgttcatcga
      181 cgaaatccat cgaatcgacc gtgttgtgga agaatacctc tattcagcga tggaggattt
      241 cgcgatcgat atcatgacgg ggacgggccc cgggtccgcc tccgtgcgag tcaacctcga
      301 gcacttcacg atgatcggcg caaccacacg gacgggcttg ctcactgctc ccatgcgcga
      361 tcgcttcagc atcatcgaac gcctgaacta ctacaccgcc gaggaactgg ctcacatcgt
      421 ccggcggtcg gcacgcattc tcaacactcc gatcgacgaa ggagggagca acgagattgc
      481 tcgccgctcg cgcgggacac cccgcattgc gaaccgtctg cttcggcggg cgcgagattt
      541 cgctcaggta cggggtgacg gaacggtgag tcaggaaata gcgacgcaca ccctgaagtt
      601 gctcgatgtt gacgaactcg ggcttgacga catggacaaa cgcatcctgc ttaccattat
      661 ggacaaattc gggggcggtc ctgtcgggct caacagcatc gcagcctctc tgaatgagga
      721 accggacacc attgaagagg tgtatgaacc gttcctcatc caggaaggcc ttctccaccg
      781 cactccgagg ggccgtgtgg tgacggtttc cgcatgccgc cattttggtc gtgcggggct
      841 cgcccaggga ggtgcgcaga gcgcattatt ctgaattcgg gggaactctt tcgcggtcga
      901 gacactctac gagataacga cgaacggctt gttgcatcca aatccggtgg cggaccgtct
      961 ggcaaggagc tcgcgataat gaatcggcag attgtatgtc tgatagtcac tctcggtgtg
     1021 tttttccagc cggtttccgc cgggtcgatg ctcgagacct atcatctgag cacgcggggg
     1081 aagctggctt ctttttccgc cattccggca ccggtcgcgt cgcttcagaa tgagtcatcc
     1141 cctcaggtga cgtccgaaga acctgtggtc acgggcaagt cacccgtgaa ggcatttttc
     1201 ctgtcgctgc tcctgcccgg tctcggggaa tggtacaccg gccacaggat ccggagcgcc
     1261 gcgtttctgg cggtggaagg aacggcgtgg ggtctctggt cgaatttccg gggcaacggg
     1321 aaggactggg aaaaccgcta catcgccttc caggccgctc actggagctt cccgaggtac
     1381 gacgcgtacc gacacgccgt atgggaccgg gttggccagc tcaccaattt ctgggaccct
     1441 tcccgtcccc tgtcagcccg gcagcaggat agcctttcgg tcctcgtggg tgcgcaccac
     1501 tatgacgatt gctgcgggca gcccatgccg tctgaggacg acaggctgga gatgatcggg
     1561 aaatactacg cgttttcgta tggctgggat gacgcgacgg tatacaacaa cccgaatcag
     1621 ctccttcggg atgtgtttcc ggctcccgcg ccgggcgata acggggtgtg ggggctcaac
     1681 cgctacgatt ggttgacggt tcttggcggc gctctcgtaa ctccggcgta cgatacctcg
     1741 tcgtatgtgt tccaggtgcc aaacctgaac cttgttcagt cggcgaaccg tgacgaatac
     1801 atgagcatgc gccagaaatc caacagcaat tacagcaatg cggagaaaat ggtgacagtc
     1861 attctgttca accacattat cagcgcaatt catgccgcgc ggctggccaa atccgggaac
     1921 gtggcaaccg gagaaatgcc gcgcgtgaac accagcctgc ggatggcgct tcagcggact
     1981 gatcgagaca tggtgccgat ggtgatgctg tggagacgat tctaatgcgc acacgctctc
     2041 ctttgtgggt atggtgtgca ttcatgacgg tcgcgtgcgt ggcccctgcg caagcggtca
     2101 gccccagggg tgccttcttt cgttccctcc tgttgccggg gtggggccag ctggcaacag
     2161 acgcgccgaa acgtgccgcc gtgtttgcgg ccgcggaagg tgccctgctt gcaggcgccg
     2221 cgggaaccgc aataatgggg aacatctatc gtgacgatta ccgggcgctg gcagcggccg
     2281 tagccggggc gaatcttgcc gggaaaggcg acggatactt cagcgatctg gcgttttacg
     2341 aaacccgtgc acaccacaat caggcagcgc tcatctacga ccagccagac ccgacactgt
     2401 atgcgccgga ggatgactgg caatggccat ccacagagga ccggatgacc taccgccggt
     2461 actggaatcg ctccagggcg atgggccagc gccttgatta cgtgttgttt gcagtgtcac
     2521 tgaaccacct tgcgtctgcg gtcgacgccg caaagcaggc ctcgagacgg aatcctggca
     2581 cttccgcgtg gcagaacgtt gttccgcggg ttggcacggc cgctgcgtcg ggccgggtgg
     2641 accttcggtg tgcatggagc ttctgaacga gcagaacgcc cgtgaggtgg acgttaatgg
     2701 gcgtcggcag gggcgctggt ggtcgggtga ttgccgggtg gatcgccatg atctgggggg
     2761 ttggcctcct cgcaacgccg gcttctgccc agtggtggaa cgagatatcc catttcggac
     2821 gcaataaggt caattaccag gcgtttgaat ggagagtaat cgaaacagag aacttccggg
     2881 tttattacta tcaaggcgag ggtgacatcg cgcgcgtggc gtcgcaaatg gcggaagacg
     2941 cgtacgcccg aatctcccgc acgctcgatt accgcctgtc ccaacccgtt cctctcctcg
     3001 tctacgactc tcacaacgac tttgaacaga ccaacgccac gtggggattg atcgacgaaa
     3061 gcacgctcgg tttcactgag gcattcaaga accgcgtagt cgttccgttc acgggctcct
     3121 atacccagtt ccgccacgtg atccaccacg agcttgtcca cgccgtgatg tttgacatgt
     3181 tctaccggcg tcagggcggg ttgagcactg cttcccagct ctggtggggt cctcctctct
     3241 ggttcgcgga agggctggcg gagtacctct cgacggattg gacgaccgaa aagaacatgt
     3301 gggttcgagg ggcaacgatc gatggatacc tgcggctcga cgggtaccag gcatacaccg
     3361 caggctactc actcctgcgc tggatcgcgg acgagtttgg tgcggacaaa atccccgcaa
     3421 taacccgccg gatggcaatc acgaaagaac ctgcggcgtc gttccgggcg gtcctcggtg
     3481 tgagccttga cgacctccaa acgcgctggg aacggcacct gaaacgtttc tactggccgg
     3541 aaatcgaacg gcgggaactg accgatgaga tgggaagacg cctcactgac caccgtaaag
     3601 accggaatca ttacaacttc gcgccggcgt tctccccgca cggcgacagg atcgcgttcc
     3661 tttccgatcg agatgactac gcctcgattt acatcatgtc ggcgatagac gggcgcatcc
     3721 tccggaaggt ggtcacgggg gaacgttcga gccatttcga agagatgcac tggtcacgcg
     3781 ggagcctcag ttggtcaccc ggcgggtctc atctcgtgtt cgcggcaaag catcacgatc
     3841 tcgacgcgtt gtatatcgtt tccgcggaaa atggccggat acagcgcatg ttccggtggc
     3901 ggcagttcaa cgcgctgttt tcgccttcct ggtctccaga cagcctgaat atcgtgttcg
     3961 tcggtgtgag agaagggcgc agcgacctgt acctggtgaa cacgctcacg ggcgaactga
     4021 ggcagcttac agatgacttc tacgacgaga ttgcacccag gtggtcgcct gatggcacgc
     4081 gcatcgcatt cgccagcgac cgggtactgg acggtgaaga ctccaacgtc ctgcccgtgg
     4141 gatttgatcg caacgtgttt gtcatggacg tgtctactgg cgtgatcaca cagatcacgc
     4201 gggcggaggg ggaggattca tctccgtgtt ggtcacctga tggccgcaaa ttggccttca
     4261 cgagtgatcg caacggaatc tacaacctgt acgtcgctga cctcgacagc ggcaccgagc
     4321 gaccactgac gaatgtgatc gacggtgtct ttcagcctga ttggtcaagg gacgggagga
     4381 agattgcggt tgccgcattc aacgtagcgg gatacgacat attcacgttg accgatcctc
     4441 tggctaaatg gcaggcggat gacccgcttc cgcccgccga gtttctggaa cgccgcagag
     4501 aagaagcgca gagcgattcc tcccgttatc tggagcgtcc ccctgtttcc ggtctggggc
     4561 actggaggtt ctcgctgcaa ccgcacgatt cctccggtaa tcctctcacc ttcgtgtcaa
     4621 agccttacga tgtgcacttc tcgccggacc tcgtctccgg acttgtgatg ttcgacaaca
     4681 tcaacggcct gatggcgcag acgatgcttc gcgtatcaga cacgatgggc gaccatcaga
     4741 tccagatcta cacccatatc taccggaatg tcgaagacgc ggacttccag tttgcgtact
     4801 acaacctccg gaagagacca aactggggat tttcgctttt ccagtttcac cagtactatc
     4861 tccaaagctt tgcggcaacg gaatggacga gcgaccgcgt actcggcggg gcggttcttc
     4921 tgaactaccc tttcaacaag ttcacccgcc tcgaaacgtc gctggagctt tctgaccgcg
     4981 agcgcgcgaa cttctcgtat gatacctatc tccgcacgcc ggtccggcgt cttcccggtg
     5041 tccgaacgct tcttccctcg ttcgcgctta tcaacgacaa tacgctttgg ggggtgaccg
     5101 gcccgacaaa cggctcccga tcagcgtttc aggtcaagtt cgcgcccgaa atcggtttca
     5161 acacctacgc gtatacgacg ctgattggcg attaccgcaa gtaccttcga gtatccgcgg
     5221 actacgcgct ggcgttacgt gtatccagcg gaatgagtgt cggtcggaac ccgggaaggt
     5281 tcctgctggg gggcacgagc tactggatca acttccacta cactccgacg tacagcgtta
     5341 cgaacgacag cctcataccg atcacccagt tcattacgcc tcttcgcggg agtcgcttcg
     5401 gtgagctttc gggaagcaga tacgcccttg cgaacatcga attcaggtac ccccttgttc
     5461 gtgagctcgc ctggggatgg cccctgccga tcgtactgcg gaacatcagg ggaatccttt
     5521 ttctcgacct gggaaccgtg tggggcgacg ggagaggttt tgctcccttc gcgggaaacg
     5581 gaatcaagct gaattacaca tctgccggca tgacgaacgc cccgacacgc ggtccactgg
     5641 cagttggtgg attcgggttc ggctggcgga tgaatctcgg cgttgcagtt ctgcgctggg
     5701 atattgcgtg gccgacggac ctcgatcgga cgcttggcgg gtcgcgtcag tactggtccc
     5761 tcggggcgga tttctgaacg ggatgaaact cccgcgcacc cgtggatgta atggtaccac
     5821 atgtgtgaat ccacccgttg gcatcacgac tcgcggtaac aagaacacaa accatgagag
     5881 tcagaaccag cgcatacgtg gccgtcctgt gcgcagccgg atttgcctcc gctggtgctg
     5941 ccgcgcccat accgtgtggc tcgcacctct tcacggcgct gcccgaatcg ttcttccgcg
     6001 aagacgtgtc ccgcgcaaaa cccgctcgca atcccgtcgt gggggcaaag gaaaccctct
     6061 ggattcagaa tttcgcggcg gggcagcttg aggaggtgtc ggtcacgtgt cgtgcagtga
     6121 accggcggag cgtcatcctc gttgcagacg cgtgctggtc tgccttgccc agcgccacag
     6181 cgattgatcc ggcggatgtg acacgcctga gtgcagcgtt cgaggagaac gcgggatcgc
     6241 cggcgccggt gggcaagggt atctacgatg tgcttgctac cgcgttcgct ccggtcaatc
     6301 ccgtaggcgg ggattcactg gtgtacatcg ctctcaccga aatcaaatcg aattacaccg
     6361 gcggctacat cgttggctac gtaagcccga acgaccgtgt tgatccctcg ttgtacgacc
     6421 gttccaactt caggaacctt gtcgttttgg acgggttgtc gcgcacgacc gccgttcagc
     6481 gggaacgcac gctcgctcac gaattcctcc acgtgattca catgggcatc gatcggtacg
     6541 aggagaggtg gatcgacgag ggaatcgccg tgctggcgca ggttctctgc gggtatcagg
     6601 gaaacacagg gcgcgagttc tttgcgaata caggtatcgg cctgttcaac gatggaacgg
     6661 cgactccaag cctgggtgat tatgacaagt cttacatgat catgcaatac ctcgcagatc
     6721 agttcggagt gcagacaatt
//
LOCUS       DAOTNG010000171         6719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9839_length_6719_cov_5.093187, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000171 DAOTNG010000000
VERSION     DAOTNG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_6719_cov_5.093187"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /gene="mutS"
                     /locus_tag="P5179_09030"
     CDS             complement(<1..2526)
                     /gene="mutS"
                     /locus_tag="P5179_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRS95399.1"
                     /translation="MTTSETRLTPMLEQYMAVKRKHQDKLILFRMGDFYETFFDDAVE
                     VSKILGITLTSRNHGKEGDVPLAGIPHHALGSYSDKLTRAGRSFVIVEQLEDPRKAKG
                     IVKRGVTEVVTPGTTLSETLLEEKRNNFLIGISVNGGVAGIAEADLSTGEFRVTEVSA
                     NELFAEVRRIGPAEVLAARSWITEHRQAFSRQVPGPVLTPMEDWAFGIAVARERLLEH
                     FKVTSLKGFGAEELTAGTAAAGAVLAYLQENQLTSLEHIRRLNVIQRAHHLILDETTQ
                     RNLELFASLSASNPRATLIGAIDQTLTPMGGRLLRQWMMRPLKDIPQINARLDGVGWF
                     VEKSMERGQVRDMLARVRDIERIMAKVCCGRAAPRELAALAASLEAAEPLSATLRDAE
                     ADLVSHAAGEMGDTSEVSALIRHTLEDDPPATLSDGGVIRRGVSADLDELREIGHGGK
                     EWLLKLQATERERTGIPSLKVAFNRAFGYYIEISNAHREKAPPDYIRKQTLVNAERYI
                     TPELKEWESRVIGAEERAISLEYELFTRLRDSVAARADAVLQQARAIALVDVLAALAE
                     TAARNNYHRPAVALDDVISIENGRHPVVEMLLPKGSFVPNDTYLANPTDQIMIITGPN
                     MAGKSTYLRQTGHIVILAHMGSWVPADKAHIGVVDRLFTRVGAHDNLAGGESTFLVEM
                     HETANILNNATPRSLLLFDEVGRGTSTFDGLSLAWAIVEYLHGTPHKAAKTMFATHYH
                     ELVELEDILPRVKNYNVTVREWQDEVIFLRKIVRGGCDHSYGIHVAQMAGLPGKVIQR
                     AREVLASLEEKDDSLHRELGAPGRSEAEGGSAGGDADAEQIGLI"
     gene            2765..3943
                     /locus_tag="P5179_09035"
     CDS             2765..3943
                     /locus_tag="P5179_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRS95400.1"
                     /translation="MKKRMIADRVYWMGAIDWDRRLFDSLIPLPDGTSYNAYLVEGSE
                     KTALLDAVDPAMADELMRQLDGVARVDYLISHHAEQDHSGTLPLLAKRFPEAKILASP
                     KARGMLLDLLDLPETAITTVTDGETLSLGDKTLKFIHTPWVHWPETMVTYLEEDKILF
                     SCDLFGSHLATGDLFAKDQAQVYEAAKRYYAEIMMPFRTIIQKNLEKIGGLDAQVIAP
                     SHGPVYDKPEWIVDAYRQWVSGAPGNVVAVPYVSMHASVERMVKHLAASLVDRGVRVE
                     LFNLAVTDVGKLAMALVDAGTIVVGTSTVLNGPHPLALYAAYLANALKPKARFISVVG
                     SYGWGGKTVDMLAETLAGLKAEVLEPVMVKGAPSADDLAAVDALAEAIAKKHAENGFA
                     "
     gene            3968..6028
                     /locus_tag="P5179_09040"
     CDS             3968..6028
                     /locus_tag="P5179_09040"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HRS95401.1"
                     /translation="MRISRACAVYIPASILFFAASPEAESIPRDANGAPVIPAVFASA
                     PPHLDGILDDPVWTESVRLDDFYVVDYDRPPSEPTTVFLACDSANLYVAARMGDRFPG
                     QIRMAETKRDGQYTNDDHFAIHFDVQHLHNTSYLFIVTPRGTQATTIPDGSPSKVEWQ
                     GDWVAAARVDSAGWSVEMVIPMVIFGRPAGQHTIGVAAHRWLPRLQEEMTWPNMGRVR
                     DDTKTGNWEGIVWPAERRRPALMPYIVAERVGGDLSTYAGADMKYTTGSGLTILTTAY
                     PDYRNIESGVLGLDFSYNEQARADNRPFVTEGAAYYPESWIFYSQRIGEMYGGGKLFG
                     QLGRSRVGLLTAYDRNRVVHTAGKWTWQKSDAWNFDQHMVWRHGPADAPHPAGTPMAS
                     DHLMYVSIARFTRQVGDREEHYSTQGGFTKTASDTANGYDFEFHWNRHNGNGTFGYAL
                     GGRILSSGFIPVEGLLNPADAGQRDITASLEWEKRRERRWFTEVGYWAQLKYSERLNG
                     DLYHKDATLEGWADVTPDVAVALNGKAVDRPPYVDKTLYVWMGWNRRSFGHEGGIEAT
                     AGKVKGTDYRFVSAYQNTRIGGQTSLSLSAQGVRHVYPVGHRDRPLGGTDDRYQVIGT
                     AQFDITPEHAVSGRLIESTQGLNGYATYQQVLRRGYDLFIIIGDPSADTWTRRVALKA
                     VVVL"
     gene            complement(6215..>6719)
                     /locus_tag="P5179_09045"
     CDS             complement(6215..>6719)
                     /locus_tag="P5179_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRS95402.1"
                     /translation="PDPTPVRYDVLEIFDPVENRWTTGPPMPSARAGLAAAAVGNRLY
                     VIGGDRDDRNLNVVEVFDPETGEWTPGPPLPTARFALGATVVGGRLYALAGSCGPACM
                     QLAEVFDPATGTWATGASLPTGRTDAAVTTCGGRIYVIGGYAISGCMDTVEIFDPRAP
                     QCGGDGE"
BASE COUNT         1243 a         2112 c         2113 g         1251 t
ORIGIN      
        1 gatcagcccg atctgctccg cgtcggcatc cccgccggct gagccgccct cggcttccga
       61 tcggccgggc gcgcccagct cccggtgaag ggagtcgtct ttctcctcaa gggatgcgag
      121 cacctcccgc gcgcgttgaa tgacctttcc cggtaatccc gccatctgag cgacgtgaat
      181 gccgtagctg tggtcgcacc cgccccgcac gattttgcgc aggaagatca cctcatcctg
      241 ccattcgcgc accgtcacgt tgtaattctt cacgcggggc aggatgtcct cgagttccac
      301 cagttcgtgg tagtgcgtgg cgaacatggt tttcgcggct ttgtgcggag tgccgtgaag
      361 gtactcgacg atcgcccatg ccagagacag cccgtcgaac gtgcttgtac cccggcccac
      421 ctcgtcgaaa agaagaaggc tgcgcggggt cgcgttgttg agaatgttcg cggtctcgtg
      481 catttccaca agaaacgtgc tttccccgcc ggcaaggtta tcgtgggcgc caacccgggt
      541 gaacagccgg tccaccacgc cgatgtgcgc cttatcggcg ggaacccacg agcccatgtg
      601 cgccagaatg acaatatgcc ccgtctgccg caggtaggtg gacttccccg ccatgttggg
      661 cccggtgata atcatgatct ggtcggtagg attggcaagg taggtgtcgt tgggtacaaa
      721 cgaccccttc ggcagcagca tttccaccac cgggtgccgg ccgttttcga tggagattac
      781 gtcgtccagc gccactgcag gccgatggta gttgttccgc gccgccgttt cggcgagggc
      841 tgccagcaca tccaccagcg cgatggccct cgcctgctgg agcacggcgt ccgcgcgggc
      901 tgcaacggaa tccctgaggc gggtgaacag ctcgtattcc agactgatcg cgcgctcctc
      961 cgcaccaata acgcgggact cccactcctt gagctccggc gtgatgtacc gctccgcatt
     1021 tacgagcgtc tgcttgcgga tgtagtccgg cggggccttc tcgcggtgag cgttgctgat
     1081 ttcgatgtaa tacccgaacg ccctgttgaa ggcaaccttc agcgacggga tgccggttcg
     1141 ttctcgttcc gtggcctgca gtttgagcag ccactccttc cccccgtgtc cgatctcgcg
     1201 caattcatcc aggtcggcgc tcacccctcg ccgtatcacg cccccgtccg aaagcgttgc
     1261 cgggggatcg tcctccagcg tgtgccgtat gagcgcgctg acctcagagg tatcgcccat
     1321 ctcgccggcg gcatggctta cgaggtctgc ttccgcgtcc ctcaatgtgg cggaaagcgg
     1381 ctccgccgcc tccaggctgg ctgccagcgc ggcaagctca cgtggtgccg cgcggccgca
     1441 gcataccttc gccatgattc gctcgatatc gcgcaccctg gcaagcatgt cccgcacctg
     1501 accgcgctcc atgctcttct ccacgaacca tcctacgccg tccagccggg cgttgatctg
     1561 cggaatgtcc ttcagtggcc gcatcatcca ctggcgaagc agccgcccgc ccatcggggt
     1621 gagcgtctgg tctatggcgc cgatcagcgt cgccctcggg ttggaggcgg agagcgacgc
     1681 aaaaagctcc aggttccgct gggtggtctc gtccagaatc agatggtgcg cgcgctggat
     1741 gacattcaac cggcggatgt gttcaaggct ggtgagctgg ttttcctgca ggtatgcaag
     1801 caccgccccc gccgccgccg taccggctgt cagctcctcc gcgccgaagc ccttgagcga
     1861 cgtgaccttg aaatgctcca gcagccgctc ccgcgcaacc gcgatgccga atgcccagtc
     1921 ctccatcggc gtcagaaccg gaccgggtac ctgccgcgaa aatgcctgcc ggtgttccgt
     1981 aatccacgag cgcgccgcca gaacctccgc cggcccgata cggcgcactt ccgcgaacaa
     2041 ctcgttcgcg gacacctccg tgacgcggaa ctcgcccgtg gaaagatcgg cctccgcgat
     2101 tccggcgacc ccgccgttca ccgaaatacc gatcaggaag ttgttccgct tttcctccag
     2161 cagcgtctcg gagagcgtcg tgccgggggt gacgacctcc gtcaccccac gcttgacgat
     2221 ccccttcgct tttcgcgggt cctccagttg ctcgacgatg acgaaactgc gcccggcgcg
     2281 ggtgagcttg tcgctgtagc ttcccagcgc gtggtggggg attccggcca gcggaacatc
     2341 cccctccttg ccgtggttgc gcgacgtgag ggtaatcccg agaatcttcg agacttcgac
     2401 cgcgtcgtca aagaacgttt cgtagaaatc gcccatgcgg aacaggataa gcttatcctg
     2461 gtgcttccgc ttcaccgcca tgtactgctc aagcatcggt gtcaggcggg tttcggacgt
     2521 tgtcatgtgc ggtcgggtat tccgtgaagg aaccgtggga gtgaacaggt acacactgtg
     2581 gaaagtatcg ctagggaagt ggcgcagacg caatgttgag aagcaggggg cagggctgtc
     2641 cgcccgcgcg cccgccaccg gggtgtagct ccacggttcg gtggttgcgt acgcgcggcg
     2701 gggggtcgga aatttagagc gggaagtaag tatcgctgtt ctcgaataac aggagaccga
     2761 gtacgtgaaa aaaaggatga ttgccgatcg cgtgtactgg atgggtgcca ttgactggga
     2821 ccggcgtctg ttcgattcgc tgataccgct gccggacggg acgtcgtaca acgcgtacct
     2881 cgtggaaggc agtgaaaaga cggccctgct ggatgcggtt gaccccgcca tggccgacga
     2941 actgatgcgg cagctcgatg gcgttgcacg ggtggattac ctcatcagcc accacgcgga
     3001 gcaggatcat tcgggcaccc tgccccttct ggcaaagcgg ttccccgagg cgaaaattct
     3061 cgcgtcgccg aaagccagag gcatgctgct ggatcttctg gaccttccgg aaaccgccat
     3121 cacgaccgtc accgatgggg agactctctc cctcggggac aagacgctca agttcatcca
     3181 cacgccgtgg gtgcactggc cggaaacgat ggtgacgtac ctcgaggagg acaaaatcct
     3241 tttcagttgc gacctgtttg gctcgcatct ggcaaccggc gatcttttcg caaaagacca
     3301 ggcgcaggta tacgaggccg cgaagcggta ctatgcggaa atcatgatgc cgttccgcac
     3361 gatcatccag aagaacctcg aaaagatcgg ggggctggat gcgcaggtca tcgccccaag
     3421 ccacgggccg gtgtacgaca agcccgaatg gatcgttgac gcctaccggc aatgggtttc
     3481 cggcgctccc ggcaacgtgg tagcggtccc ctatgtgtcc atgcacgcca gtgttgagcg
     3541 catggtgaaa catcttgccg cgtcactggt ggatcgtggc gtgcgcgtgg aacttttcaa
     3601 cctcgcggtg acggacgtcg gcaaacttgc catggcgctc gtggatgccg gaacaatcgt
     3661 ggtaggcacc tccaccgtgc tgaacggccc gcacccgctg gccctgtatg ccgcctatct
     3721 tgcgaacgcc ctcaagccga aggcgcgatt catttccgtg gtgggttcgt acggatgggg
     3781 cggcaaaacc gtggacatgc tggcggagac gctggcgggt ttgaaggccg aggtcctgga
     3841 gccggtgatg gtgaaaggcg caccatcggc ggacgatctt gcagcagtgg acgccctggc
     3901 agaagcgatt gcgaagaaac acgcggagaa cgggttcgcg tagattctcc gcgttcacag
     3961 gggttttatg cgcatcagca gggcgtgtgc ggtatacatc ccggcgtcta tccttttctt
     4021 tgcggccagc ccggaggcgg agtccatccc gcgggacgca aacggtgcgc cggtgattcc
     4081 ggcggtgttc gcctcagcgc caccccacct tgacggcatc cttgacgacc ccgtctggac
     4141 ggaatccgtc cgccttgatg atttctacgt ggtggattac gaccgtcccc ccagcgaacc
     4201 caccacggtg ttcctggcat gcgactccgc caatctctat gtggccgccc ggatgggcga
     4261 caggttcccc gggcaaatcc gcatggcgga gaccaaacgg gacggccagt acaccaacga
     4321 cgaccatttc gccattcact tcgatgttca gcacctccac aacaccagtt acctgttcat
     4381 cgtcacgccg cgcggcactc aagcgacaac tatccccgac ggcagcccca gcaaggtgga
     4441 gtggcagggg gactgggttg ctgcagcgag ggttgacagc gccgggtggt cagtggaaat
     4501 ggtgatcccg atggtgattt tcggccgacc ggccggccag cacaccatcg gggtagcagc
     4561 ccaccggtgg ctgccgcgcc tgcaggagga aatgacctgg ccgaacatgg gtcgcgtccg
     4621 cgatgacacg aaaacgggca actgggaagg gatcgtgtgg ccggccgaac gccggaggcc
     4681 cgcgctcatg ccgtacatcg tggcggagcg cgtcggtggg gacctgagca cgtacgccgg
     4741 cgcggacatg aagtacacca ccggctccgg tctgaccatc ctcaccacgg cgtaccctga
     4801 ttaccgcaac atcgaatctg gcgtactggg tctcgatttc tcctacaacg agcaggcgcg
     4861 cgccgacaat cgcccgtttg tcaccgaggg agcggcctat tacccggaga gctggatatt
     4921 ttacagccag cgcatcgggg agatgtacgg cggcgggaaa ctgttcggac agcttggcag
     4981 gagccgcgtc ggcctcctga cggcctatga ccggaaccgt gtggtacata ctgccggaaa
     5041 atggacctgg cagaaatcgg atgcctggaa tttcgatcag cacatggtct ggcggcacgg
     5101 ccccgcggat gcccctcatc ccgccggcac acccatggcg agcgatcatc tcatgtacgt
     5161 atccatcgcc cggttcacgc ggcaggtggg agatcgggag gagcactaca gcacgcaggg
     5221 gggattcacg aaaaccgcca gcgataccgc gaacgggtat gacttcgagt tccactggaa
     5281 ccgccacaac ggcaatggaa ccttcgggta tgcgcttggc gggcgaattc tgagcagcgg
     5341 gttcatcccg gtggaggggc ttctgaaccc ggcggatgcg ggtcagcggg atatcactgc
     5401 gtctctggaa tgggaaaaaa ggcgtgagcg acggtggttc accgaggtgg gatactgggc
     5461 gcagctcaag tattcggaac ggctcaacgg tgatctctac cacaaggacg cgacgctgga
     5521 aggctgggct gacgttacgc cggacgtcgc ggtggcgttg aacggaaagg cggttgatcg
     5581 ccccccgtac gtggacaaga cgctgtacgt gtggatgggc tggaatcggc gctcattcgg
     5641 ccatgagggc ggcatcgaag cgaccgccgg caaggtgaag ggcaccgatt accggttcgt
     5701 ttccgcctac cagaacacgc gaatcggcgg ccagacctcc ctcagcctga gcgcgcaggg
     5761 cgtgcgccac gtatacccgg tgggacacag ggatcggccg ctgggcggca cggacgaccg
     5821 gtaccaggtg atcggcacgg cccagtttga cattactccg gaacacgcgg tatcggggag
     5881 gctcatcgag agcacgcagg gactgaacgg gtatgcgacg taccagcagg tgcttcgccg
     5941 gggctacgac ctgttcatca tcatcggcga tcccagcgct gatacgtgga cgcgccgcgt
     6001 ggcgctcaag gcggtggtgg tgctgtgagc gccacaagta gcgcgcaatg cgcgcaaaat
     6061 cgcgctcatt tgtgagtgcg gcgcccgccg cactcaccgc ttgtgacgac ggtgcccgag
     6121 cgttctggat tccgcgtgcc cctggtgcgc gagcattccg tcattcctgc gtgctcgacc
     6181 gagcacgaag cgcaggcggg aatccagagg cactttattc cccatcacct ccgcattgtg
     6241 gggccctggg gtcaaaaatc tccaccgtgt ccatgcaccc cgagatggca taccccccta
     6301 tgacgtagat cctgccgcca caggttgtca ctgcggcgtc cgtacggcct gtcgggaggg
     6361 atgcgccagt cgcccacgtt ccggtggcgg gatcgaagac ttcagctaac tgcatgcatg
     6421 ccgggccgca actccccgcc aaagcataca gtctcccgcc aacaaccgtt gccccgagcg
     6481 cgaaccgggc ggttggaagt ggcgggccgg gagtccattc cccggtttcc ggatcgaaca
     6541 cctcaaccac attcagattg cgatcgtctc tgtcaccgcc gatgacatac aacctgttgc
     6601 ccaccgcggc cgcggcgagg ccagcgcggg ctgatggcat gggaggacct gtcgtccacc
     6661 tgttctcgac gggatcaaag atttccagaa cgtcatacct caccggagtg gggtctggc
//
LOCUS       DAOTNG010000172         6652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9952_length_6652_cov_5.658178, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000172 DAOTNG010000000
VERSION     DAOTNG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9952_length_6652_cov_5.658178"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5179_09050"
     CDS             <1..464
                     /locus_tag="P5179_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95403.1"
                     /translation="DPVAVRSLVKDDRRFVYFVNREYYPVAVTVAFNQAPGNVINAAT
                     GESEPGLSPWMVVLSPYELRAIIVAPEIALTGFEARPPAEIASVLEQRAREALADIES
                     VRLSGVLVPGMDEMEEGISRAMEEKRYAWLRRALDGYIVRKCRALAADRSE"
     gene            complement(543..1778)
                     /locus_tag="P5179_09055"
     CDS             complement(543..1778)
                     /locus_tag="P5179_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95404.1"
                     /translation="MPTKQDIAIIRELARQKAEIAALPVQEEKRALWRKLNGLKPDRP
                     MVAIDQVCWNEMNVNDELTLRCADPELRGYEDRLRRELFQWRHFPGDMVVDPYIHVSK
                     AIHNTGFGVRVQERVAVTDPTSGVVGHKYTNQFETEEDLQKIQIPRLTHDEAETARQL
                     EFAHELFDGIIDVRLWGVDAYLSLWDPISTWMSVESALLAIVDRPDYVHRLVGRMTDG
                     YMAMFDQAEAEGLLCGPQNWVHCTGAFTDELPAPGYNPTKPRTKDMWAMGLAQMFSTV
                     SPKIFKEFEVDYVSRLCARFGLVYYGCCDPLDGKMNEVRMIPNVRKVSMSPWVNEERG
                     ATEIAGDYVYSRKPSPAFLAHDSFDAEAVRSDLQKTRDICERYGCPLEYILKDISTVR
                     YQPQRLFKWSEVAMEVACG"
     gene            complement(1935..3632)
                     /locus_tag="P5179_09060"
     CDS             complement(1935..3632)
                     /locus_tag="P5179_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRS95405.1"
                     /translation="MDLDLARTPFSRYGSYFVFSYLDRPKTRQPGLYLRTVRGGNPTH
                     DLLRVELLVDGTPAPFTINANTTILTLEAGPASVSFCIAEQTAIRIKGNGAGVRFTHA
                     PIAAFDNVFLVDSATAEVNDWSSGIRLAFSVPTGSLNADAPWQNPRCTHMVIDCLPDS
                     GDGVLDCVVEEFKTTRPLRNRPCPTFDECTKSVAAEFRKWAKRSPKVSMVHENAAELA
                     QYLTWSSVVAPSGRMNRPAMLMSKNWMSNIWCWDACFNAMALAYRNPELAWDQFMLFF
                     DHQDQNGALPDSLNDVSIVWNFTKPPVHGWVLRWMMERSDFITRRELKQVYAPLAAWT
                     NWWMTARDDDGDGVPQYNHGNDSGWDNATVFDVGLPVESPDLSAYLVIQMDVLSEIAD
                     ALGRGRDARRWKKRADTLLGKMISHSWRDGQFVAPRSGQHDVAEGDCLLPFVPIVLGK
                     RLPKEQRKALVAGVLRDGRFLTEHGLATESPASAKFDPKGYWRGPIWAPATLLIVDGL
                     VALGERGAARQISKRFCDMVLAEGMAENFHPHNGKGLCDPAYTWTASVFQILAHEYV"
     gene            complement(3832..5139)
                     /gene="melA"
                     /locus_tag="P5179_09065"
     CDS             complement(3832..5139)
                     /gene="melA"
                     /locus_tag="P5179_09065"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS95406.1"
                     /translation="MPKITFIGAGSLGFFKTLMIDCLSFEALRNSDWALVDIDPKRLD
                     YARRIGERIMRDAGSTGKLVATLDRREVLKGSDFVIMAILVGGYEPIAFDIDIPMKYG
                     VDQCIGDSHGPGGVFRALRTIPVKVGIARDIADLCPNAWVLNYTNPMSMLCRAMDIET
                     GINLVGLCHSVQGTTGMLADWAGVPRDEIDYWVAGINHQAWVLRIRHNGEDLYPKIKE
                     AARKPEHWNSLTASCEMLEHLGYFVTEGSGHNSEYNAWFRKTPESVAKYCPGGSWNGG
                     SGFIKQLYGSDREGWEADQERFASDTSPIVFRRSHEYGSNIINGIQTHVPFRFNGNVA
                     NKGLITNFPQDVMVEVPCFADKNGVKPCHVGALPPQLAALNSMNINVIELAVQAALKG
                     DRDAAFHACCYDPLTMAVLDLRQIRSMVDEMFEAEKPWLPQFA"
     gene            5508..>6652
                     /locus_tag="P5179_09070"
     CDS             5508..>6652
                     /locus_tag="P5179_09070"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRS95407.1"
                     /translation="MNVLVINCGSSTIKYQLINTDDQQALAAGSVERIGMADAVLSHQ
                     PQGREKVRVTGEILDHIQAIEYTLAILLSPQYGVIRDTSEIDAVGHRVVHGGEKFSGS
                     VILTDEVMAQIRECIELAPLHNPHNVRGIEACQRLMPKVPEVAVFDTAFHQTMPDYAY
                     MYALPWKLYERHAIRRYGFHGTSHGWVAARTAELLGRPISSLKMVTCHLGNGCSIAAV
                     DGGKSVDTSMGFTPLEGLVMGTRSGDIDPAIILHVIGKEDLNVHEATALLNKHSGMLG
                     LSGVSGDMREIEAQVEAGDPKATLALRVYCYRIRKYIAAYAGAMGGLDTIVFTAGVGE
                     NSSLVRKLSCEGLTFMGVELDDTLNGTRSKTGRVISTDTSRVKVVVMP"
BASE COUNT         1343 a         1891 c         2024 g         1394 t
ORIGIN      
        1 ctgatcctgt cgcagtgcgc agccttgtca aggacgaccg gcggttcgtg tacttcgtga
       61 atcgcgaata ctaccccgtg gccgtgaccg tggcgttcaa tcaggcgccg gggaacgtga
      121 tcaatgcggc taccggggag tccgagccgg ggttatcgcc ctggatggtt gtcctttctc
      181 cgtatgaact tcgggcaatc atcgtagcgc cggaaatcgc gctgaccggg ttcgaggccc
      241 ggccgccggc ggaaatcgcg tcagtgctcg agcagcgcgc ccgcgaggca ctggcggaca
      301 tagagtccgt gaggctttcg ggggtcctgg ttccgggtat ggacgagatg gaagagggca
      361 tatcgagagc gatggaggaa aagcgctacg cgtggcttcg ccgagcgttg gacgggtaca
      421 tcgtgcggaa gtgccgggcg ctggccgccg acaggagcga gtgaagggtg aggcccggga
      481 atgcagggtc cggctcccac acgcgagatg tgacccgaac ctctggatta cgcgtggtca
      541 acttacccgc aggcgacttc catcgcaact tcggaccatt tgaagagccg ttgcggctgg
      601 tagcgaaccg tgctgatgtc cttaagtatg tattcaagcg gacatccgta tcgttcgcat
      661 atgtcccgcg ttttctggag gtcgctcctc accgcctccg cgtcaaagga atcgtgcgcg
      721 aggaatgccg ggctgggttt tcgtgaatac acgtaatcgc cggctatttc cgtggcacca
      781 cgttcctcgt ttacccacgg gctcatggac accttgcgga cgttcggaat cattcggact
      841 tcattcattt tcccgtcgag cgggtcacag cagccgtagt acacgaggcc gaatcgcgcg
      901 cacaggcgac tgacataatc cacctcgaat tccttgaaga ttttcgggga gacggtggag
      961 aacatctgcg cgagccccat cgcccacatg tccttggtgc ggggctttgt cgggttataa
     1021 ccgggggccg ggagctcatc ggtgaacgcg cccgtgcagt gaacccagtt ctgcggaccg
     1081 cacaggagac cctccgcttc tgcctggtcg aacatcgcca tatagccatc tgtcattcgg
     1141 ccgaccaacc tgtggacgta atccgggcga tcgacgatcg cgaggagggc gctttccaca
     1201 ctcatccagg tgctgattgg gtcccacagc gaaagatagg cgtccacacc ccagaggcgg
     1261 acatcaataa ttccgtcgaa cagctcatgc gcgaactcaa gctggcgggc ggtttcggct
     1321 tcgtcatggg tcagccgcgg tatctgaatt ttctgcaggt cttcttccgt ctcaaactgg
     1381 tttgtgtact tgtgacctac gacgccactt gtggggtctg tgacggcaac acgctcctga
     1441 acacggacgc cgaacccggt gttatggatg gccttggaga cgtggatgta cgggtcgacc
     1501 accatgtccc cggggaagtg ccgccactgg aacagctcgc gacgcagacg gtcctcgtac
     1561 ccgcgcaact cggggtcggc gcaccggagc gtgagttcgt cgttcacatt catctcattc
     1621 cagcacacct ggtctatcgc taccatgggg cgatccggct tgagcccgtt caatttgcgc
     1681 cacagtgcac gcttttcctc ctgcacgggc agggcggcga tttcggcctt ttgtcgggcc
     1741 aattcgcgaa tgatcgcgat atcctgtttg gtcggcatgg gatgagtcct tcgcatacgt
     1801 gacgggtcag ggatgggaca tacaggacac aagatagggc gcaacaaggc aagggcaaag
     1861 atgggaggat tgacagccgg gattcccttc gagcgcggag gccggtaacc ctggaagaaa
     1921 cgagatgagc gttcctacac gtattcgtgt gcaagaatct ggaatacgct tgccgtccag
     1981 gtgtaggccg gatcgcacag gccttttccg ttgtgcgggt ggaagttctc cgccatacct
     2041 tccgcaagca ccatgtcgca aaaccgtttg ctgatctggc gagcggcgcc gcgttcaccg
     2101 agcgccacca gaccatctac gatgaggagg gttgcgggcg cccagattgg tcctcgccag
     2161 taccctttcg gatcaaactt ggcgcttgcc gggctttcgg tggccagacc gtgctccgtg
     2221 aggaatcgtc catccctgag cacgccggcc acaagggctt tgcgctgttc ctttggaaga
     2281 cgcttaccga gaacaatcgg tacgaagggg agcagacaat ccccttccgc aacatcgtgc
     2341 tgcccggacc gtggagcgac aaactggccg tccctccatg aatgggagat catttttccc
     2401 aggagcgtat ccgcccgctt tttccagcgt cgggcatctc gtcctctgcc cagcgcatcg
     2461 gcgatttccg aaaggacgtc catctgaatc accaggtacg ccgaaaggtc gggggattcg
     2521 acgggaaggc ccacgtcgaa aaccgtggcg ttatcccagc ccgagtcatt tccgtggttg
     2581 tactgcggaa caccgtctcc atcgtcgtcg cgcgcggtca tccaccagtt cgtccacgcg
     2641 gcgaggggag cgtatacctg tttcagttcc cgcctcgtga tgaaatccga ccgttccatc
     2701 atccaccgaa gcacccatcc gtgcacggga ggcttggtga aattccacac aatcgagacg
     2761 tcgttcaggc tgtcaggaag cgccccgttc tggtcctgat ggtcgaaaaa cagcatgaac
     2821 tggtcccagg ccagttctgg attccggtat gcaagcgcca tggcattgaa gcaggcatcc
     2881 cagcaccaga tgttcgacat ccagttcttg gacatgagca tcgcgggacg gttcattctg
     2941 cctgacgggg ctacgaccga tgaccatgtg aggtactgcg cgagctccgc cgcgttctca
     3001 tgaaccattg agaccttggg agaacgcttc gcccatttcc ggaactccgc tgcaacggat
     3061 ttcgtgcact catcgaacgt ggggcagggc cgattgcgca ggggacgcgt cgtcttgaat
     3121 tcttccacca cgcaatcaag gacaccatcg cccgaatcag gcaggcagtc gatgaccatg
     3181 tgagtacacc gcgggttttg ccagggcgcg tccgcgttga gcgatccggt aggcacggag
     3241 aaggccagcc ggattccgct ggaccaatca ttcacctctg cggttgcgga atcgacaagg
     3301 aacacgttat cgaacgccgc aataggcgcg tgtgtgaaac gaacccccgc gccgttcccc
     3361 ttgattcgaa tcgccgtctg ttcggctatg cagaaggata cactcgccgg gcctgcttcg
     3421 agagtgagaa tcgtggtgtt cgcgttgatc gtgaaaggag cgggtgtccc gtcaaccagc
     3481 agttcgacgc ggagaagatc atgcgtcgga tttccgccac gaacggttcg caggtaaagg
     3541 cccggttggc gggtcttggg ccggtcaaga tacgagaaga cgaaatagga gccgtaccgg
     3601 ctaaatggcg tacgcgcaag atccaagtcc atggccgtcc tctggttgtg ttccagcagc
     3661 gtacttcgcc cgcgaatgga ggctacgaag gcatatccct caatgtaccg aattttgtca
     3721 cgtcaatgtt acgattttgt gaggaacggc tcctgatcag ttccgccgat aaagaaagcg
     3781 cccgtccgaa tccggacggg cgcgagagat cgacggatga tgaacctatg gtcaggcgaa
     3841 ctggggcagc cagggcttct ccgcttcgaa catttcgtca accatggacc ggatttgccg
     3901 cagatccagc actgccatgg tgagcgggtc gtaacagcac gcgtggaagg ccgcgtcacg
     3961 gtcgcctttg agcgccgcct ggaccgcaag ttcgatcacg ttgatgttca tgctgttcaa
     4021 tgcggcgagt tgggggggca aagcgccgac atgacagggt ttgaccccgt ttttgtctgc
     4081 gaaacacggt acttcgacca tcacgtcctg cgggaaattc gtgataagac ccttgtttgc
     4141 cacgttgccg ttgaaacgaa acgggacatg ggtctggatt ccgttgatga tgttggaacc
     4201 gtattcgtgc gaacggcgga acacgatcgg gctggtgtca gatgcgaatc gttcctggtc
     4261 ggcctcccag ccttcccggt cgctgccata gagctgtttg atgaaaccgg agcccccatt
     4321 ccaactccct ccggggcagt atttcgccac tgactccggt gttttgcgga accacgcgtt
     4381 gtattcgctg ttgtgcccgc tcccttcggt gacgaaatag cccagatgct ccagcatttc
     4441 gcaacttgcg gtaaggctgt tccagtgttc gggtttgcga gcagcttcct tgattttcgg
     4501 gtaaaggtcc tcgccgttgt gtcgaatccg gagaacccac gcttgatggt tgataccggc
     4561 cacccagtag tcgatttcgt cccggggcac acccgcccag tcggcaagca tccccgtggt
     4621 tccctgcaca ctgtggcaaa gcccgacgag gttgattccg gtttcgatgt ccatcgcacg
     4681 gcagagcatg ctcatcggat tcgtgtagtt cagcacccac gcattcgggc aaaggtccgc
     4741 aatgtcgcgg gcaattccta ctttcacggg aattgtccgg agcgcccgga acacgccgcc
     4801 gggaccgtgg ctgtcgccaa tgcactgatc cacgccgtat ttcatcggga tatcgatgtc
     4861 aaatgcgatt ggctcatatc ccccgacgag aatcgccatg atgacgaaat cgctcccttt
     4921 aagtacttcg cggcgatcaa gggtagcgac cagtttgccc gtgctccctg cgtcgcgcat
     4981 tatgcgttcg ccaatgcgcc gcgcgtaatc gaggcgcttg ggatcaatat ccacgagcgc
     5041 ccagtcgctg ttgcgcaacg cttcgaagct gaggcagtca atcatgaggg ttttgaaaaa
     5101 accaaggctt ccggcaccga tgaaggtgat cttgggcatc tggattcctc cgggggaagg
     5161 ggagagttcg cggcgtaacg ggtacgaaaa gtcacaaatc atgaagaaac acttgaggtt
     5221 caggtaatac ggtagccact accaggaacg gcgtcaagcc taattcgggc agtttgttgg
     5281 cgcgggagcc atgccggggc gaaatcggag cgatggggta gggaattccg cgaccggcgg
     5341 cggggaggtg agggttgaac cgcggtatcc tccatggtac cctatgtaat gatgattgac
     5401 ttccacaact tcctcgtgcc cccggggcca tttttgagcc gtttctcgat gggattaccc
     5461 ttcggcggtg ccatcgggcc gcacttcctt ccaacaggat gactcgaatg aacgtactcg
     5521 tgatcaactg cggcagttcc acaatcaaat accagcttat caacaccgat gatcaacagg
     5581 ctcttgcggc gggttcggtc gaacgtatcg ggatggcgga cgcggtgttg tcgcatcagc
     5641 ctcaggggcg ggaaaaggtc cgggtgacgg gtgagattct tgatcacatt caggctattg
     5701 agtacacgct ggcgattctt ctgagcccgc agtacggggt gataagggac acctcggaga
     5761 tcgacgccgt cggccaccgc gtggtgcacg gaggggaaaa gttttccggc tcggtgattc
     5821 ttacggacga ggtcatggcc cagatccgag agtgtattga gctggctccc ctgcataacc
     5881 ctcacaacgt gcgcggcatc gaagcctgcc agcgcttgat gccgaaggtg ccggaagtcg
     5941 cggtgtttga cactgcgttc caccagacga tgcccgacta cgcgtacatg tacgcattgc
     6001 cctggaagct ctacgaacgg catgcgatcc ggcggtacgg gttccacggc acatcgcacg
     6061 ggtgggtggc agcccggact gcggaattgc tcggaaggcc gatttcctcg ttgaaaatgg
     6121 taacgtgcca tctggggaac gggtgctcca tcgcggcggt tgacggcggt aaatcggtgg
     6181 atacctccat gggatttacg ccgctcgaag ggttagtcat gggcactcgg tcgggggaca
     6241 tcgaccccgc gatcattctg catgtgatcg gcaaggaaga cctcaatgtt cacgaagcaa
     6301 cggcgctcct gaacaagcac agcggcatgc tcgggctttc cggagtatcg ggagatatgc
     6361 gtgaaatcga ggcccaggtt gaggcgggag accccaaggc aacgcttgct ctgcgagtgt
     6421 actgctatcg cattcgaaag tatatcgccg cgtacgcggg cgccatgggc ggcctggaca
     6481 cgattgtctt cactgcgggt gtgggagaga actcgtctct ggtccggaag ctctcctgcg
     6541 aagggctgac gttcatgggc gtcgaactgg acgacacgct taacggcacc agaagcaaaa
     6601 ccgggcgcgt tatttccacg gacaccagca gagtgaaagt cgttgtcatg cc
//
LOCUS       DAOTNG010000173         6642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_9967_length_6642_cov_4.950964, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000173 DAOTNG010000000
VERSION     DAOTNG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_6642_cov_4.950964"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..823
                     /locus_tag="P5179_09075"
     CDS             182..823
                     /locus_tag="P5179_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95408.1"
                     /translation="MRFPQAVTFKGTMRVISYDPRAGMRLERMPESRFRLWFGLFFSV
                     GLACIGVLLFARTAFENYRASGALNWLDFLLIVIGLICVAFGLIVALPLGLRFVPKVI
                     TTTHEPPLVCLRQFRTHTWRPQDVRGLHLVGRQYLRRTDPDAPSSWYVFSLVLQKSKG
                     RDRLLLQTLPQPDYEAGFQDIVPIATYFSELLATPLTTVCNWDAPAHITRRRT"
     gene            856..1683
                     /locus_tag="P5179_09080"
     CDS             856..1683
                     /locus_tag="P5179_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95409.1"
                     /translation="MVTLTGFGDEISPDLTEQMDVMESEGIRHIELRAVWNTGVHKLS
                     EEQRQKIKEETQRRGFRISSLGSGLGKIKVDDDFGPHVRDCEVLIQTAKFLGTPYIRI
                     FSFYVPQDQPVTAHRDEVMRRVRVLTDMAAAEGIVLGHENERHIYGEKPEQCRDLLDT
                     INSPSLRAIFDPANFVQAGVRPFEDAWPMLKDDVLYFHIKDAKQADGRVTPAGEGDGG
                     IKAILADKLASGWEGFLSLEPHLKIAGPAGGYSGPLAFKTAAQALKRLLDEIGVRYH"
     gene            1701..2540
                     /gene="iolB"
                     /locus_tag="P5179_09085"
     CDS             1701..2540
                     /gene="iolB"
                     /locus_tag="P5179_09085"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRS95410.1"
                     /translation="MPETQTDFHIRKTLLHGENVIVPANHPSLELLRFSLLCLNEKES
                     HSFCLDEEEAALVILTGTASIRVNDSVFERIGGRRDVFSGNAATVYAPCHSKIVVDCA
                     GPGPLSLAVCCSKSTLRREPALIAPQDVKTKTVGRANWTRHVKDIIDVSFDAGHIVVG
                     ETINPPGNWSSSPPHRHEVDNPPDEVKMEEIYFFQLKPQQGFGFQRIYTDDRSLDVSY
                     AVEHNSTVILPKGYHPVAAAPGYQLYYLWFMAGPTSRTLCPKDDPAHSWLKAAEPIID
                     GLK"
     gene            complement(2675..2896)
                     /locus_tag="P5179_09090"
     CDS             complement(2675..2896)
                     /locus_tag="P5179_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95411.1"
                     /translation="MGLLYERDRRVTKDKNLPEIKETDTMKRSGLVVLPTREHDIEEF
                     TEEWDELDLFEDEIVDEDVDPEEVDEEAF"
     gene            3176..3835
                     /gene="rpe"
                     /locus_tag="P5179_09095"
     CDS             3176..3835
                     /gene="rpe"
                     /locus_tag="P5179_09095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRS95412.1"
                     /translation="MLLPRTRLIAPSLLAADFGDLRNEISRVEKAGADWLHLDVMDGH
                     FVPNLTFGPFIVSAIRSLTPLPLDTHLMITDPAFYAPRFAESGSTLITFHVEAVQDVP
                     RTIREIKALGVNAGLSIKPATPVDVVLPYLESLDLVLVMSVEPGFGGQKFMPEAVPKI
                     RSLREAIDARNLSTKIQVDGGINPETAKLVRDAGVDVIVAGTSVFRSTDPAAALAQLR
                     A"
     gene            3994..4470
                     /locus_tag="P5179_09100"
     CDS             3994..4470
                     /locus_tag="P5179_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRS95413.1"
                     /translation="MLIADRVTGYEEWEERVLAGETGALLDRILSAPGVNLAAEPVCV
                     TALVACRSKRGTLPGVAEIRACGARLQALVTVIQPVIIVALGLCTGKALVRSGIAEPA
                     IDGRDYDVFRGIPVRTTCHPAEALWGVPKEVNRIKRILYQDWKAISAHYFALKTTR"
     gene            complement(4517..>4821)
                     /locus_tag="P5179_09105"
     CDS             complement(4517..>4821)
                     /locus_tag="P5179_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRS95414.1"
                     /translation="ALEIDSVPHVLEHGLWQTSTVGIITGGGGRFVKEAAHAGIDTFI
                     TGEGPHFSALDAEEFGMNVIHAGHYATEKLGVKALAAHVADKFGVETQFIDHPTGI"
     assembly_gap    4822..4921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5338..5961
                     /locus_tag="P5179_09110"
     CDS             5338..5961
                     /locus_tag="P5179_09110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRS95415.1"
                     /translation="MPLPVLSLASATAALRRGEVVAYPTETFYGLAVLPSDAEALARL
                     VRLKGRDTDKPVSLILGKAEVVSDFATSLTPTTIRLISLAWPGPLTVVLPAKDSVARV
                     ITGGTGTVAVRVPSHAGARMLADSAGGAITATSANRQNMPPARTAEEVEAQFPDGLAG
                     VFNGERAPGDLPSTLVRPVGGTLHLLRRGAVDLPTLREWWSGEIVDA"
     gene            complement(6034..6312)
                     /locus_tag="P5179_09115"
     CDS             complement(6034..6312)
                     /locus_tag="P5179_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95416.1"
                     /translation="MTLYNQPNAFGITGISQCPIYDPGCGSREDWFGLSSESPVCADM
                     SRIAELAALDLRSRGKRRNLRKDDDREVVLSKERLAQGWFHRSERDRN"
BASE COUNT         1377 a         1947 c         1781 g         1437 t
ORIGIN      
        1 ctttgccatc gcgcaccgtc tcagcacgct gaagaacgca aaccgcctgc tcgtaatcga
       61 ggacggacag gtggtcgaat tcggaacgca cgacgaattg ctgctcaaag agggtgtgta
      121 cgcgaagctt gtaaatgccc agcggcagtt gtcctcaata actgctgtgg gggggtgagg
      181 cttgcgtttt cctcaggcgg tcacgttcaa aggcacgatg cgggtgattt cttacgaccc
      241 gcgcgcgggt atgcgcctgg aacgaatgcc ggaatcgcgg tttcgcctgt ggttcggcct
      301 ctttttcagc gtcgggcttg cgtgcatcgg tgtgctcctt ttcgcgcgaa cggcgtttga
      361 aaactaccgt gcctcgggag ctcttaactg gcttgatttc cttctgatag taattggcct
      421 catctgtgta gcgttcgggc tcatcgtcgc gttgcctctc ggattacgat ttgtcccgaa
      481 agtgatcacc actacacacg agccgcctct cgtttgtctg cggcaatttc gcacccacac
      541 gtggcggccg caggatgtac gcggtctgca tcttgtgggc cgacaatacc tcaggcgcac
      601 cgacccggac gccccttcct cctggtatgt gttcagtctc gtgctgcaga aatcgaaggg
      661 acgggacaga cttctgctgc agacactgcc ccagcccgat tacgaagccg gctttcagga
      721 tatcgttcct atcgcgactt atttttcgga gctcctcgca actccgctca ccactgtgtg
      781 taactgggat gcgccggcgc atatcacccg gcgacgcacg taaggtttgt ccctcgcaaa
      841 ggaggatact cgaacatggt aactctgacc gggtttggag atgaaatatc gcccgacttg
      901 acagaacaga tggacgtgat ggaatccgaa gggattcgcc acatcgagtt acgggcggtc
      961 tggaacacag gtgtccataa gctcagcgaa gaacaacgcc agaagatcaa ggaagaaacg
     1021 cagcggcgcg gatttcgcat ttcctcgctc ggttccgggc tcggcaaaat caaggttgat
     1081 gacgatttcg gcccgcacgt gcgtgattgc gaggtactta ttcagacggc gaagttcctc
     1141 ggcacgccct acatacgaat cttcagcttt tacgttccgc aagaccaacc ggtgaccgcc
     1201 cacagggatg aagtcatgcg gcgggtcagg gtgctgaccg acatggctgc cgccgaaggg
     1261 atcgtcctcg ggcacgaaaa tgaacgccac atctacgggg aaaagccgga gcaatgcaga
     1321 gatctgctcg atactatcaa cagcccatcg cttcgtgcga ttttcgatcc ggcgaacttc
     1381 gttcaggctg gcgtgcgacc atttgaggac gcgtggccga tgctgaagga tgatgtcctc
     1441 tactttcaca tcaaagatgc caaacaggca gacggacgtg tcacacccgc aggcgaagga
     1501 gacggaggaa tcaaggcaat ccttgccgac aaactggcgt cgggatggga aggctttctc
     1561 tcgttggagc cgcacctgaa aatcgccggc ccggcagggg gttattccgg accattggcc
     1621 ttcaaaaccg ccgcgcaggc actgaagcga cttctcgacg aaatcggcgt gcgctaccac
     1681 tagatgccag gaggaaatag atgccagaaa ctcaaacaga ctttcacatc cgcaagacgt
     1741 tgctccatgg agaaaacgtc atcgttccgg ccaatcaccc gtcgcttgag ttgctccgtt
     1801 tttcgctgct gtgcctgaac gaaaaggaat cacactcgtt ctgtctggat gaagaagaag
     1861 ccgctctggt cattcttact gggacggcgt ccatcagggt caacgattcc gtttttgaac
     1921 ggatcggcgg tcggcgtgat gtattctccg gaaacgccgc caccgtgtat gccccatgtc
     1981 acagcaagat cgtggtcgac tgcgccggcc ccggacctct ttctctggct gtctgttgct
     2041 ccaaatcgac gttgcggcgc gaacccgcat taatcgcccc gcaggacgtg aaaacgaaaa
     2101 cggtggggcg ggcgaactgg acgcggcacg tcaaagatat catcgacgtg tcattcgacg
     2161 cgggacacat cgtggttggg gaaaccatca atccgccagg aaactggtct tccagtccac
     2221 cgcacaggca cgaggtggac aacccgccgg acgaagtgaa aatggaggag atctatttct
     2281 ttcaattgaa accgcaacag ggattcggct tccagcggat ctataccgat gaccggtctc
     2341 tcgacgtcag ctacgcggtg gaacacaatt ccacagtcat attgccaaaa ggataccacc
     2401 cggtggctgc cgcacccggt tatcagcttt actacctctg gttcatggcg ggaccgacga
     2461 gcaggacgtt atgcccgaag gacgatcccg cgcacagctg gctgaaagct gccgaaccga
     2521 taatagacgg gctcaaataa gcaaagaaga acaaaaggag ggggttgacg gtaacgctgg
     2581 gaagcggttg gtaccattac ttttcaaaca ccgactcaaa cgccattcga cgtccgcagt
     2641 ggggttgcgt cctcagactc gcaacccctg cgggctagaa cgcttcttcg tccacttctt
     2701 ccgggtcgac gtcctcgtcc acgatttcgt cttcaaacag atcgagttcg tcccattctt
     2761 ccgtaaattc ctcaatgtcg tgttcacggg tgggaagaac gaccagcccg gaacgcttca
     2821 tcgtatcggt ttccttgatt tccggaagat tcttgtcttt tgtgacccgg cgatctctct
     2881 cgtacaacaa acccattggc tttcctcctt cgtcgacatt acctgcgcgt cgcgtaatgc
     2941 aaaagccacg ccaataaaag gcttgcctga agcagttctt gccacggacc tttcttcgcc
     3001 ttggcaccac cgcgaaatct gttgccttga gccgcttctg ggtccttctc gacacgaacc
     3061 atcgtatgca atagtaagcg cgttgtcaag agccactgtg cgcggtatcg aatgaagctg
     3121 tatttttatt tctcccgtac atgtccgtaa gtcccgaatc ccgaggaaca tgttcatgtt
     3181 gctcccgcga acacgtctca ttgctccatc tcttctggcg gccgatttcg gcgatctgcg
     3241 caacgaaatc tcccgagttg aaaaggccgg tgctgattgg ctccacctcg acgtaatgga
     3301 cggccatttt gtcccaaatc tcacgttcgg ccccttcatc gtgtccgcga ttcggagtct
     3361 cacgccgctt ccgctggata cccacctcat gattacggac cccgcctttt acgctccacg
     3421 tttcgctgag tcgggcagca ccctcatcac cttccacgtc gaagctgttc aagatgtgcc
     3481 tcgaacaata cgcgagatca aggccctcgg agtgaacgcc gggttaagca taaaacccgc
     3541 aacacccgtc gacgtggtgt tgccctatct cgaaagcctg gacctcgtgc ttgtaatgtc
     3601 ggtggaaccc gggttcggag gccagaagtt catgccggaa gcggtaccga aaattcgctc
     3661 gctccgcgag gcaattgacg cccgcaatct cagcacaaaa atccaggttg atggcgggat
     3721 aaacccggag acggcgaagc tggtccgtga tgccggagta gacgtcatcg tcgccggcac
     3781 ctccgttttt cgcagcaccg accccgctgc ggcgctcgcg cagcttcggg cgtgatatcc
     3841 gtgctcttta ccggtttccc tcttctcgaa gcggctgccg ttcccgggag cctttcggag
     3901 ctacaccgcg aattcgcccg gtgtcacaga tgcagtctgg cggatacccg cagacgtgtt
     3961 ctctgcgcct cccgcttcaa gcgcggcggg ttaatgctga ttgccgatcg tgtcacgggg
     4021 tacgaagaat gggaagagcg ggttctcgcc ggagaaaccg gtgcgctgct ggaccgtatt
     4081 ctctcagccc ccggagtaaa tctcgcggca gaacctgtgt gcgtaaccgc gctcgttgcc
     4141 tgccggagca aaagaggcac actccctggt gtcgcggaaa tacgggcctg cggggcccga
     4201 ttgcaggctc ttgtaaccgt gattcaaccc gttattattg ttgcacttgg tctgtgcacg
     4261 ggcaaagcgt tggttcgttc tggcatcgcc gaaccggcta ttgatggccg ggattacgac
     4321 gtcttccggg gaatccccgt cagaaccaca tgccacccgg ctgaagcgct ctggggggtg
     4381 ccgaaagaag tcaatcgcat aaaacgcatt ttgtaccaag actggaaggc aatttcagcg
     4441 cactattttg cgctgaaaac gacgcgttag cgcaaaatat gcacggtgtt ctcaaaacgt
     4501 cgggtccgtg aaaccgtcaa attccggtgg gatgatcgat gaactgggtt tctacgccga
     4561 atttgtctgc cacgtgggcc gccagcgctt tcacaccaag tttttccgtg gcgtaatgac
     4621 ccgcatggat cacgttcatt ccgaactctt ccgcgtccaa tgcggaaaaa tgcggcccct
     4681 cacccgtgat gaatgtgtcg attcccgcgt gcgcagcttc tttaacgaag cgcccgccac
     4741 cgccggtaat aatcccgacc gtagacgttt gccagagtcc gtgttcgagg acatgaggca
     4801 cggaatcaat ctccagtgca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 natggtcgcg aggcatgcat caacggcgac tgcaaccctc cggatatctg cagcaccctc
     4981 gacctgaagc ccgttgagag cctcttctga gtcgggtatc tcgtcgatcc gcaggtattc
     5041 gttcaagtag ttgacgaaat ctccaagtgg gacgctcata gtaaccttgg ctcctgcaaa
     5101 acggccccgc ccccaacaaa acgggaactt cccacatcgt tatgaacaga cacggaattc
     5161 agaccttccc tcaccgcgct cggagccggc aatcgtaccg gtcacgcgcg taggatgctc
     5221 cacactccgg aagcccttgt ccgcattatc aagacttgcg aatgttactc cttgatcacg
     5281 gtaatgtcaa ttccctgtcc ttcaggaaac ccttgtgcag atcgagaggc acgtaacatg
     5341 ccattgcctg ttttgtccct tgcatcagct actgccgccc tccgtcgcgg cgaggtagtc
     5401 gcctacccga cagaaacctt ctacgggctt gctgtcctcc catccgatgc ggaggcgctg
     5461 gcccgcctcg tcagactgaa aggaagagac accgacaaac ccgtaagcct tatcctcggg
     5521 aaggccgaag tggtatccga cttcgcaacc tctttgaccc ccaccaccat acgtctgatt
     5581 tctctcgcgt ggcccggccc ccttacggtc gtcctcccgg cgaaagactc ggtggcacgc
     5641 gtgatcaccg gcgggacagg taccgtcgcg gtccgcgtac cttctcacgc cggtgcgagg
     5701 atgctcgctg attccgcggg aggtgccatc acggcgacga gcgcgaaccg tcagaatatg
     5761 ccgcccgcgc gaacggcgga agaggtggaa gctcagttcc ccgacggcct cgccggggtg
     5821 ttcaacggag aacgcgcacc gggagacctc ccgtccacgc ttgtgcgccc cgtcggggga
     5881 acactgcacc tgctgcggcg gggcgctgtt gatctgccca ctctgaggga atggtggagt
     5941 ggcgaaatcg tggatgcgta gagcatccag aaaaaggaaa ggaaccgtgc cacacagccc
     6001 acggttcctt ccccttcgag tagagcgttc atgtcagttt cggtcgcgtt ccgatcgatg
     6061 aaaccacccc tgcgcgagac gctctttcga aagaacgact tcccgatcgt cgtccttcct
     6121 gagattgcgt ctttttcccc ttgaccggag gtcgagggct gccagctctg cgatgcgtga
     6181 catgtcggcg cacacgggcg attcggacga cagcccgaac caatcctccc gcgaaccgca
     6241 gccagggtcg tatatcggac attgcgagat tccggtgatc ccgaacgcat tgggctgatt
     6301 gtataacgtc attgttcgct cctttgagtt ttcctttcgc tcgttgttcg ccgtcggttg
     6361 tgacgacggg cgcttccggg gttccgtgct ccggttgtcc cttgcacctc gaagaacccg
     6421 gaattctagc atgcaatgac tgtgccattg ttgcacttca actctacatg cccacattta
     6481 cgctatgtgt accctccttg gtgctgtttt tcgccacgca accgcgcaag aattgcattt
     6541 tgctgccatt gatgcactat ccgccaccaa taggcggatc cccagcaaac agagcacttc
     6601 ctccagttgt cggtctggct ctatactagg aatgcacgaa tc
//
LOCUS       DAOTNG010000174         6617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10019_length_6617_cov_4.828406, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000174 DAOTNG010000000
VERSION     DAOTNG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10019_length_6617_cov_4.828406"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="P5179_09120"
     CDS             complement(<1..1199)
                     /locus_tag="P5179_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS95417.1"
                     /translation="MNTRRLTGEAAVYTFGIVLDTLAPFLVLPILGRYLTPGQFGLAD
                     VITATAGLTYFAVGFNLDQALTRYYYDEEDAHRKRLVTTHLAVVSVSGTVFLVLGLVG
                     LHAFRGSLQDLSAWHWALLSVPLMVMSDQAATLFRLQHQPFKQLGLLSVRAFVWVFFT
                     SFFLVWLRAGISSIFIGRVAGAIFGLGASAYALRRLYALRGNLRFAVRALRFSLPTLP
                     AAFASWGLVNAARYFLVIGASAEETGYYGVGVRLTYIVSTIGIGVVMAWGPFFMGIKE
                     EPNAREIQARGLLYFIAANVVCIAPLLMFSREAVLLLMGSQYLPAVRLVGPMLAATLF
                     SNLSLFLFVQISVSERTHWQSVSYAVGLVVMVIANVLLVPAMKGMGAAIAMLCGHLAT
                     AGTMWFAG"
     gene            complement(1216..2394)
                     /locus_tag="P5179_09125"
     CDS             complement(1216..2394)
                     /locus_tag="P5179_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95418.1"
                     /translation="MDAHIENTPAAEGGKSAFASFLALLWRGRLVIVGTFIVVVTVVT
                     IAVFVMTPSFTAKASFMPPPDDAGSVSSFLRDPLSAVLKRGSGGAVLDRLASFLNSQT
                     TRRILVERFNLWPHYKARNLSEAMIALEKQTRVVVTTEGTIELAVTDRDPRLAAALAN
                     CYLDVVDSLFRDAQTSHARQMREFLERRLNENRRALAEAESTAKAFSLRYGVVALPEQ
                     VASLVGLMASIEGKIMALDVQIGAARQVLGPDHFSLRELTSQREQLAAQRKALIETKG
                     RGTSDPLFAFREVPERALQWARIEREIKIQTLIQELVLQQYEVAKLEEQRDVSSLTII
                     DRATPPEIRSWPRRGRIIAISAAIAIVWGILLAYALEEYPRLRRRIREMNDLHSRSDA
                     "
     gene            complement(2394..4013)
                     /locus_tag="P5179_09130"
     CDS             complement(2394..4013)
                     /locus_tag="P5179_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRS95419.1"
                     /translation="MNRRNILTLLSVAALFFSTQITALTPWAGDEEERQDRPPASEER
                     DRTAQQSVSRAVSGTPLDMRVDREKYRLDPGDRVLVSLWGATNKSFELVVTPEGDLLV
                     PAVGPVAVARLSLAEAERTLARQAANAYRNTRITLSLVALREFRVHVAGTVKRPGTYL
                     GTPADRVSSVIRLAGGFADSASMRRIQLLRTGLPPRLIDLQMFSVTGDTTDNPSLEMG
                     DVITVPVRGDSVSVWGAVRKPGGYEYRPGDTVLRLIRLAGGLRDDAQTAEVEWSSFPV
                     DSAPTVVNKVSLDRLIAAGSDTVVRPGDRLMVYPVAEWRRRQSVTVEGEVRFPGEYSI
                     IEGKTRISDVIARAGGPTRSASLVGSRILRRRVQAEPDQEFLRLSRMPVADMKREEYA
                     YFKARSRELRGYLSIDVGTALESPGGVDDAPLVDGDLIVIKRARATIEVAGQVRRPGL
                     IEFDEGRSTQFYIDQAGGVLSNAHRRGIRVIKQGTGLWVKPSADLRLEPGDTIFVPEK
                     ESVEWWELFKEGLLVVSQIATTVFIIQSVVR"
     gene            complement(4003..5025)
                     /gene="rfbB"
                     /locus_tag="P5179_09135"
     CDS             complement(4003..5025)
                     /gene="rfbB"
                     /locus_tag="P5179_09135"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRS95420.1"
                     /translation="MSWTILVTGGAGFIGSNFVRYLLEKYPGYRVINLDKLTYSGNLN
                     NLKDIARDPRYEFVHADIAEESVVFPLVQRSDAVVNFAAESHVDRSIADPGVFLRTNI
                     EGPHVLLEASRKSGVKRFVQISTDEVYGEVMTGSSRETDRLAPRSPYSASKAGGELLV
                     QSYHVTFGVPTLVTRGSNTIGPYQYPEKVVPLFITNALDDRPLPIYGPGTAVRDYIHV
                     VDHCRGIDQVLHHGVEGEVYNIGADNSVNTVDLASAIVDYLGKPRALLQHVQDRPGHD
                     QRYSVDSGKLRKLGWNLSYTFQDALEETIRWYRDNREWWEPIKKGGYARWYNQHYKIR
                     ETPHES"
     gene            complement(5022..5810)
                     /locus_tag="P5179_09140"
     CDS             complement(5022..5810)
                     /locus_tag="P5179_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS95421.1"
                     /translation="MELYVLASSSAGNAIAIRNGQGIMLVDAGLSARRIEERLHAVGW
                     HAQDVKCVLLTHEHSDHTRGVGVFARRYHVPVYGTPGTLQAITDQWRGTESLYAVAAG
                     SRTVIHGFQVEAFSVPQDVSDPVQFVIRDGLVSVGIATDLGTVTSLVRQKMRAVDLAV
                     VEANHDAERLRWGRYPWNVKQRVGSSQGHLDNEQAGELVRDLAREGVPHIVLAHLSPQ
                     HNDAEHVEKAMANALATVAQRPVLTIVPPGEGSPVIQVRKAVRS"
     gene            complement(5810..6430)
                     /gene="rdgB"
                     /locus_tag="P5179_09145"
     CDS             complement(5810..6430)
                     /gene="rdgB"
                     /locus_tag="P5179_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRS95422.1"
                     /translation="MPCQIIAATRNRGKLREIQSLYADLPLTWRPMDDVGSPPIIEET
                     GTTFLENALLKARGIAQWSGCAALGEDSGLEVDALAGDPGVRSARFAGEHASDEENIA
                     LLLRRLDGIPSAERTARFVCVVALVWTDGTEVIAKGTCEGHIAEERAGASGFGYDPLF
                     IPEGQVATFAELGEEFKNTVSHRARALRALRPWLEGRIRYAGRAPQ"
     gene            complement(6436..>6617)
                     /locus_tag="P5179_09150"
     CDS             complement(6436..>6617)
                     /locus_tag="P5179_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95423.1"
                     /translation="SNDPTKYAFITIGAHLDKDITEGPELEGLRKRLGSPVVNCMVFS
                     VSKINVVHLPKPKPA"
BASE COUNT         1364 a         2057 c         1868 g         1328 t
ORIGIN      
        1 cctgcaaacc acatggttcc cgcggtggcg agatgcccgc acagcatcgc gatggcggct
       61 cccatccctt tcatcgctgg caccagcagc acgttcgcga tcaccatcac cacgaggccg
      121 acggcgtagc tcaccgattg ccagtgcgtc cgctcagaaa cagaaatctg gacaaacagg
      181 aaaagcgata gattcgaaaa gagagtggcc gccagcatcg gaccgaccaa tcgaaccgcg
      241 ggaagatact ggctgcccat aaggagaagc actgcctcac gtgagaacat cagaagcggc
      301 gcgatgcaga ccacgttcgc agcgatgaag tacaacagac ctctcgcctg aatttctctc
      361 gcattgggct cttctttgat tcccatgaag aaaggccccc atgccatcac cacgccgatc
      421 ccgatcgtcg aaacgatgta cgtcaggcga accccgactc cgtagtaccc cgtttcttcc
      481 gcagaagccc cgatcacaag gaaatatcga gcggcgttta ccaggcccca gctcgcgaag
      541 gccgcgggaa gtgtcggaag tgaaaacctc agcgcccgga cggcgaagcg aaggttgcca
      601 cggagcgcgt acagacggcg aagagcgtac gcgctggcac ccaacccgaa aatggccccc
      661 gcgacccttc cgatgaaaat ggaagaaatc ccggcacgca accagacgag gaaaaaggac
      721 gtgaagaaca cccatacaaa cgcgcggaca ctcaacagcc cgagttgttt gaatggttgg
      781 tgttgcaggc ggaagagtgt ggctgcctga tcgctcatca ccataagggg aacactcaac
      841 aacgcccagt gccaggctga cagatcctgg agactgccgc ggaatgcgtg cagtcccacg
      901 aggcccagaa cgagaaagac cgtgccggat acggatacca ccgcgaggtg tgtggtgacg
      961 agtcgcttgc ggtgggcatc ctcttcgtcg tagtagtaac gagtcagcgc ctgatcgagg
     1021 ttgaacccga ccgcgaagta ggtcaacccc gcagtggccg taatgacgtc ggcaagtccg
     1081 aactgcccgg gggtgaggta ccgaccgagg atggggagaa caaggaaggg agcgagggtg
     1141 tcaaggacga tcccgaaggt gtaaacagct gcttcccccg tcaatctccg cgtgttcatt
     1201 ccggcatggt ttccgtcagg cgtcggagcg ggaatggaga tcgttcattt cccggatgcg
     1261 ccggcgcaac cgggggtact cctcaagggc atacgccagc agaattcccc agacgatggc
     1321 aattgcggcg gaaatcgcga taatcctccc tctgcggggc catgaccgga tttccggtgg
     1381 cgtggctcgg tcaatgatcg tgagtgagga aacgtccctt tgttcttcca gttttgccac
     1441 ctcgtattgt tgcaaaacga gttcctggat caaggtctgt atcttgatct cccgctcgat
     1501 acgagcccac tgaagagccc tttccggcac ttcacggaac gcgaacagcg ggtcggaagt
     1561 ccctcgccct ttcgtctcga tcagtgcttt cctctgcgcg gcgagttgct ccctctgact
     1621 cgtcagttcc ctgagggaga aatgatccgg accaagcacc tgccgtgccg cgccgatctg
     1681 cacgtccagc gccatgatct tgccctcgat agaggccatc agacctacca ggctggcaac
     1741 ctgttcaggt aatgccacca cgccatacct gaggctgaat gccttcgccg ttgactccgc
     1801 ctccgcgagc gcgcgtcggt tctcgttgag acgtcgctcc aggaattcgc gcatctgccg
     1861 agcgtggctg gtttgtgcgt cacgaaacag actgtcgaca acgtccagat agcagttggc
     1921 aagcgccgcg gccagtcttg ggtcgcggtc agtaaccgca agctcgatag tcccttctgt
     1981 tgttacgacc accctggtct gcttttccag agcaatcatc gcttcggata gatttctcgc
     2041 cttgtaatga ggccaaaggt tgaatcgttc caccaggatc cttctagtgg tctggctgtt
     2101 cagaaaactg gccagacgat ccagcaccgc tccgccagaa ccccgcttca gcacggcgct
     2161 caacgggtcg cgcaagaaag atgatacgct tccggcgtcg tccggaggcg gcatgaacga
     2221 cgcttttgcc gtaaacgatg gcgtcatgac aaagaccgcg atcgtgacca cggtgacgac
     2281 aacaataaac gttcccacga tgaccagccg gcctcgccac agaagcgcaa ggaacgaggc
     2341 gaaggccgat ttgccgcctt ccgccgccgg ggtgttttcg atgtgtgcat ccatcagcgg
     2401 accacgctct ggatgatgaa cacggtagtt gcgatctgcg acacgacgag aagcccctct
     2461 ttgaaaagct cccaccactc gaccgattct ttttccggga cgaagatcgt atctccgggt
     2521 tccaggcgca ggtccgcaga tggtttgacc cagaggcctg taccctgttt tattactcga
     2581 atgcccctcc tgtgagcatt tgaaagcact ccgcccgcct ggtcgatgta gaactgcgtg
     2641 gatcttcctt catcgaactc aatcagcccg ggccttctga cctgccccgc gacttcgatc
     2701 gtggcgcgag cgcgcttgat tacgatcaaa tcgccatcga caagcggtgc atcgtcaaca
     2761 cccccgggac tttccagcgc agttccgacg tcgatggaaa ggtacccgcg caactcgcgt
     2821 gatctagcct tgaaatacgc gtattcttct cgcttcatgt ccgcgacagg catcctcgac
     2881 agcctgagga attcctggtc cggctccgcc tgcacacgcc gcctcaggat gcgggagccg
     2941 acgagagacg cactcctggt gggcccaccg gcgcgcgcaa taacgtcaga aatcctcgtc
     3001 ttcccttcga tgatcgagta ctcgccgggg aagcgaactt cgccctcgac cgttaccgac
     3061 tgcctgcgtc gccattctgc gacgggatac accattaacc tgtcgccggg acgcaccacc
     3121 gtatcggacc ccgccgcaat gagacggtcc aggctcacct tgttcactac tgtgggagcg
     3181 gaatccaccg gaaagctgga ccattccact tcggcggtct gggcgtcatc tcgcagccct
     3241 cctgccaggc ggatcagacg caggaccgta tcccccgggc gatactcgta tcctcccggt
     3301 ttgcgcaccg cgccccacac actcaccgaa tccccgcgga cgggcacggt gattacgtca
     3361 cccatttcca gagaagggtt atccgtggta tcgccggtga cggaaaacat ctggagatca
     3421 atcagccttg gcggcaagcc cgtgcgcagg agctggatgc ggcgcatgga agcgctgtcg
     3481 gcgaatcctc cggcgaggcg tattacagaa ctgaccctgt ccgcgggtgt gccgagatag
     3541 gttcccggtc tcttcacggt gcccgcaacg tggacacgga attcccgcag cgcgacaaga
     3601 ctcaaagtga tgcgcgtgtt gcgatacgcg tttgcagcct gccgggcgag ggtacgctcg
     3661 gcctccgcga gggaaagccg agctacggca acaggaccta ctgccggaac cagaaggtcg
     3721 ccttcaggcg tgacaaccag ttcgaacgat ttgttcgtag ctccccatag gctcacgaga
     3781 acgcgatccc ccggatcgag acggtatttc tcccggtcta cgcgcatgtc caaaggagtg
     3841 cccgaaactg cgcgggaaac gctttgctga gccgtccgat cccgttcttc ggacgccggc
     3901 ggccgatcct gccgttcctc ctcgtctccg gcccacggcg tcagcgcggt gatctgcgtc
     3961 gaaaagaaca gcgcagctac ggaaagtaac gtcagaatat tcctacgatt catgcggagt
     4021 ctcgcggatt ttgtagtgtt ggttatacca gcgtgcgtat ccacccttct tgatgggctc
     4081 ccaccactcg cggttatcgc ggtaccagcg tatcgtctcc tccagcgcat cctgaaacgt
     4141 gtaagagagg ttccatccca gcttgcggag tttgcccgag tcaacagaat aacgctggtc
     4201 gtggccgggc ctgtcctgta cgtgctggag gagcgcacgc ggcttgccga ggtaatccac
     4261 gatggcggat gccagatcaa ctgtgttcac cgagttgtca gcaccgatgt tgtacacctc
     4321 gccttcgacc ccgtgatgca gaacctgatc gattccacgg caatggtcga ccacgtggat
     4381 gtaatcacgc actgccgtcc ccggcccgta gataggcaga ggacgatcgt cgagagcatt
     4441 cgtgatgaac agcggaacca ctttctctgg gtactggtac ggcccgatgg tgtttgatcc
     4501 ccgggtaaca agcgtcggca caccgaatgt tacgtggtag gactggacga gcaattcgcc
     4561 tcccgctttg ctcgcggagt aaggactcct cggcgccagc cgatctgttt ctcgggaaga
     4621 ccccgtcatc acctcgccgt acacctcgtc ggtgctgatc tgcacaaagc gcttgacacc
     4681 actctttcgg gaggcctcga gcaacacgtg cggcccttcg atgttcgttc ggagaaacac
     4741 ccccgggtcc gcgatggatc ggtctacgtg gctttccgcc gcaaagttga caactgcgtc
     4801 ggaccgctgt acgagcggaa ataccacgga ttcctccgcg atgtccgcgt gaacgaactc
     4861 gtatcgagga tcccttgcga tgtccttgag gttgttcaga ttgccggaat atgtgagctt
     4921 gtcgaggttg atgacgcggt aacccgggta tttttccaaa aggtagcgca cgaaattgga
     4981 cccgatgaat cccgcgccgc ccgttaccag tatggtccaa ctcatgaacg cacggccttt
     5041 cgaacctgga tcacaggact tccttcgccg ggtggaacga tagtcagcac cggtctctgt
     5101 gccacagtgg caagtgcgtt tgccatagct ttttccacgt gttcggcgtc gttgtgctgg
     5161 gggctgagat gcgcaaggac tatgtgagga acgccttccc gtgccagatc gcgcaccagc
     5221 tcgcctgcct gctcattgtc caggtgtccc tgagagctgc ccacacgttg tttgacgttc
     5281 cacggatatc ggccccacct caatcgttcg gcgtcgtggt ttgcttctac gactgcaagg
     5341 tccacggctc gcatcttctg ccgaacgagg gaagtaactg tgcccaggtc ggttgcgatc
     5401 ccgacagaca cgaggccgtc acgaataaca aattgaaccg ggtccgacac gtcttgcgga
     5461 acggaaaacg cttccacctg aaagccgtga atgacggtcc gtgaccccgc cgcaacggcg
     5521 tacaggctct cagttccgcg ccattggtca gttatagcct gcagggttcc cggggttccg
     5581 tatacgggaa catggtaccg ccgcgcgaag acccccactc cccgcgtgtg atcgctgtgc
     5641 tcgtgggtga ggagaacgca cttcacgtcc tgcgcgtgcc acccgacagc gtgcagccgt
     5701 tcttcaatgc gtcgggcact gagacctgca tcgacgagca tgatgccttg cccgttccga
     5761 attgctatcg catttccggc gctgctgctc gcaagaacgt acagttccat cactggggtg
     5821 cacgacctgc gtatctgatt cgcccctcca accagggacg aagtgcccgc aaagctctgg
     5881 cgcgatggct cacggtgttc ttgaactcct cgcccagttc ggcgaaggtc gccacctgtc
     5941 cttcagggat gaaaagagga tcgtacccaa aaccacttgc tcccgcacgc tcttccgcga
     6001 tatgcccttc gcaggtccct ttcgcaatca cttcagtgcc gtccgtccag acgagggcga
     6061 cgacgcagac gaatcgtgcc gtgcgttcgg ctgagggaat cccgtcgagt cggcgcaaca
     6121 ggagagctat gttctcctcg tccgatgcgt gttccccggc gaagcgtgcg gaacgaacgc
     6181 cgggatcgcc tgccagtgca tctacttcca ggccgctgtc ttcacccagc gccgcacagc
     6241 cgctccactg tgcaatgcct cgagctttga gaagggcgtt ttccagaaat gtggtgccgg
     6301 tttcctcgat aatcggcgga cttccgacgt catccatcgg ccgccacgtc agaggcagat
     6361 cggcatacag agattgaatc tctcgcagtt tgcccctgtt gcgcgtagcc gcaatgattt
     6421 gacagggcat acccgttatg ctggcttcgg tttcgggaga tgcaccacgt tgattttgct
     6481 taccgagaac accatgcagt tcaccaccgg cgaaccgagc cgtttccgca acccctccaa
     6541 ctcgggacct tccgtgatgt ccttatccag atgggcgcct atggtgatga acgcgtactt
     6601 cgtcggatca ttgctcg
//
LOCUS       DAOTNG010000175         6557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10120_length_6557_cov_5.879114, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000175 DAOTNG010000000
VERSION     DAOTNG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10120_length_6557_cov_5.879114"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1647)
                     /locus_tag="P5179_09155"
     CDS             complement(187..1647)
                     /locus_tag="P5179_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95424.1"
                     /translation="MDALREIGDRLELFVDDWLIDELHGARQLLHQPVPREPALVTDR
                     PWEGNMCHYVTVLRDGALCRMYYSTWHAQFDRNADGSVSLTHTKGNIACVESLDGIHW
                     HRPEYGLFEVGGSRRNNLVWAGEGDEIRGTHGFAPFIDVNPSCPADQRYKAVGRAEKK
                     RDLWAMASPDGIRWRILGDKPILSGLPFDSQNLVFYDALRGEYRAYVRDFDNGVRGIR
                     TATSPDFVNWTEPKWLEYPGAPVQQLYTNQIMPYPRAPHIFLGFPTRYTERKWSPAIE
                     ALPELEHRRLRAGASERYGAAVTDGLFMASRDGQTFHRWNEAFLRPGLRTEGSWTYGD
                     MYQAWGLLETESDIAGAPPEFSLYVVEGYWRGNANLIRRYTLRMDGFVSVNAPFAGGE
                     MVTKPFTFSGSRLTLNMSTSAAGSIRVEMQGANGKPLEGFALDDCVEVIGDELARTVS
                     WKGGPDVSRFAVQPVRLRFVLSDADLYAIQFAGHGG"
     gene            complement(1663..3234)
                     /locus_tag="P5179_09160"
     CDS             complement(1663..3234)
                     /locus_tag="P5179_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95425.1"
                     /translation="MSDATHMLLHKAFQIGHVPILGSYTVPTVEAIEKAASMGMNTII
                     AGQMHLDLAAPEGKACRQRGIRVLYPLEKFVYHGVSLRNEISAEQTEIPLFLAPRHHF
                     SDSLVIALDDELIRYERVENGTLIGSARGWNGTRPARHREGTILFWPEDCEQEVLRVK
                     DSPNLLGWYVLDDSPGDARSALRAMYRIVRKYDPEIRQPVCAGYGDAGSVLNFGPGIC
                     DIMMIYWYPVEPDRYMRESTAEEVQWILAKARERVPGVPFIGVFQSFDGRPMNSGQGL
                     PTSEQVREQMEDFVREGACGLIAFQSSKWGWIDTPELEKPIAKAYREIRQTSGLFVRP
                     ETKEMARRRTQPQGFWTTPNHVPGYVPAWRVAAPFDVPEGKTLDAVLPPDETIDFDAV
                     YEGPFGEIRWRTWETVGGEMEISNLFNRGLIVHKTAYAVSDVENDRERDALMGISSDD
                     DCIVRINGTEVYRYEGSGGVTRDKFKVHVRLPKGRFRLFVKCHNRLGGWGFSLRFMDL
                     AGKPLKGLRFDPERQ"
     gene            complement(3358..3939)
                     /locus_tag="P5179_09165"
     CDS             complement(3358..3939)
                     /locus_tag="P5179_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HRS95426.1"
                     /translation="MNLESLEQRIARVVREDVAIVPYDPAWPRMFAEEKAHLLASFPD
                     GLIRRIEHFGSTAVPGLAAKPIIDMLVEVRSLEETKTRIAPVLEAQGYDYFWRPLFGD
                     NVPPFYAWFIKRDRAGSRTHHIHMVEADSALWERLLFRDYLIAHPETAKEYEELKLRL
                     AREYPNDRVAYTNGKSAFCIRITRLARERVRPH"
     gene            complement(4127..4561)
                     /locus_tag="P5179_09170"
     CDS             complement(4127..4561)
                     /locus_tag="P5179_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS95427.1"
                     /translation="MPTVRLAEGPIIVFLTVCAKERKMILATREAHQTLCEAWEATRG
                     WLVGKYLIMPDHVHLFCSPAGPDHPELSVWVKCWKTYASIRWPRRSEQPIWQKSFWDT
                     QLRRSERYEDKWDYVVQNPVRKRLCGNPNEWPFQGEINVLPW"
     gene            complement(4702..5982)
                     /locus_tag="P5179_09175"
     CDS             complement(4702..5982)
                     /locus_tag="P5179_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95428.1"
                     /translation="MNLLFIFTDQMHRYALRCMGTPDIHTPNLDALAQRGMLFERAYS
                     NCPVCTPFRINLFTGLYSSQTGTMKNAARIPAGCVTLADALNSGGYETCYVGKWHIGG
                     AGNGPIPKELRGGFSDFIGYQCYNGFIHNVCFYDRDDREHCFDRHRTDVTTDLAIEKL
                     RGLAPHPFALFVSYQAPHYPVQPSPEFERMYAGVTIQRRPNSQEIDPYTRTWSPPSPW
                     PPDSCPDFQRYGNNLNEYLRLYYALVTQIDAGVGRILAELERLGRAEDTVVVFTSDHG
                     DMQGSHGLKNKSLPYEESSGIPLIVRVPGGIGGGRTGALVSGIDFFPTALDWLGLPQV
                     PGLPGRSFAPLSRGETQELNGPVFSEMRDWKMVCMGDLKLVVEGEQFTPTMLYDLAAD
                     PYEMENLVDRREYAAEVAALRERIVDWQAQSVTG"
     gene            6180..>6557
                     /locus_tag="P5179_09180"
     CDS             6180..>6557
                     /locus_tag="P5179_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95429.1"
                     /translation="MDLLDHGFWDYTTPYAGGMEAYRRDDYLRLVDDMAEAGMNSLMI
                     CIKWLTTGYRSRLPFLDQLPGNPVIESDNGLLRAAIAAAHDRGMRVWLGAVVSIFVVE
                     NYGGAPYETSDRLPLGFPIVPFGL"
BASE COUNT         1192 a         2126 c         1955 g         1284 t
ORIGIN      
        1 cagcaaaacg gctgtgggac aggctgccct gcctttcgag atctgttccc tccaagggcc
       61 tttcgcccgg cctataccaa tctccggcac cgatagcgtc attcccgcga aggcgggaat
      121 ccagaaacag caatggcagc accgcgcctg ccggcccttc agactccgaa gcgccacctt
      181 tccgcttcac cctccgtggc cggcaaattg aatcgcgtac aggtcggcat ctgacagcac
      241 gaaccgcaaa cggaccggct gaaccgcgaa acgggacaca tcagggcccc ccttccacga
      301 taccgtccgc gccagttcgt cgccgatcac ctcaacgcag tcatccagcg cgaagccttc
      361 cagcggcttg ccattcgccc cctgcatttc cacccggatg cttcccgccg ccgacgtgga
      421 catgttcagc gtaagccgcg agccggaaaa cgtgaacggc ttcgtcacca tctcgccgcc
      481 ggcaaacggc gcgttcacag acacgaaccc atccattcgc agcgtgtagc ggcggatgag
      541 gttggcattg ccgcgccagt agccctccac aacgtagagc gagaactccg gcggcgcgcc
      601 cgcgatatcg gactccgttt ccagcagtcc ccacgcctgg tacatgtcgc cgtatgtcca
      661 gctcccttcc gtgcgcagcc ccgggcggag gaaggcctcg ttccagcgat ggaacgtctg
      721 gccgtcgcgg ctggccatga acagaccgtc cgtcacggcg gcgccgtacc gctccgacgc
      781 tcccgcgcgt aaccggcggt gctccagttc gggcaaggcc tcgattgccg ggctccattt
      841 gcgctccgtg tagcgcgtgg gaaagcccag gaaaatgtgc ggggcgcgcg gatacggcat
      901 gatctggttc gtgtagagct gttgcaccgg cgcgcccgga tactccagcc acttcggctc
      961 cgtccagttg acgaaatccg gtgaggtcgc cgttctgatg ccccgcactc cgttgtcgaa
     1021 atcgcgcacg tatgcccggt actccccccg cagggcgtcg taaaacacca gattctgcga
     1081 atcgaacggc aggccgctga ggatcggctt atccccgaga atccgccagc gaatgccgtc
     1141 cggcgacgcc atggcccaca ggtcgcgctt tttctcagca cgccccacgg ccttgtaacg
     1201 ctggtccgcg gggcatgaag gattcacgtc aatgaacggc gcaaacccgt gcgtgccccg
     1261 gatttcatcc ccttctccgg cccagacgag gttgttccgc cgtgagccac ccacctcgaa
     1321 cagaccgtac tcgggacggt gccagtggat gccgtccaga ctctccacac aggcgatgtt
     1381 gcccttggtg tgcgtgagcg agaccgagcc atcagcgttc cgatcgaact gcgcgtgcca
     1441 cgtggagtaa tacattcggc acagcgcgcc gtcgcgcaga accgtgacgt agtggcacat
     1501 attgccttcc cacggcctgt cggtgacgag ggcaggctcc cgcggcacgg gctggtggag
     1561 cagttggcgg gcgccgtgca gttcgtcaat gagccagtcg tccacgaaaa gctcaagccg
     1621 gtcgccaatt tcgcgcaggg catccatcgg gcgtcccttt ccttattgcc gttcggggtc
     1681 gaaccgcagg cctttgagcg gctttccggc gagatccatg aaacggagcg agaaacccca
     1741 accaccaagc cggttgtggc atttcacgaa caaccggaaa cgccctttcg gcaacctgac
     1801 gtgcaccttg aacttgtccc gtgtcacgcc gccgctgcct tcgtagcggt atacctcagt
     1861 gccgttgatg cgcacgatgc agtcgtcgtc gcttgagata cccatgagcg cgtcgcgctc
     1921 gcggtcgttt tccacgtcgc tcaccgcgta cgccgttttg tggacgatca gcccccggtt
     1981 gaacaggttg ctgatttcca tctcaccgcc gacggtttcc cacgtccgcc accggatctc
     2041 cccgaacgga ccctcgtaca cggcatcgaa atcaatcgtc tcgtcgggcg ggagcactgc
     2101 gtccagtgtc ttgccctcgg gcacgtcaaa cggagccgcc acccgccatg ccggaacgta
     2161 gccgggaacg tggttcggcg ttgtccagaa gccctgtggt tgcgttctgc gccgcgccat
     2221 ttccttcgtc tccggccgga cgaaaagccc gctggtctgg cggatttccc ggtacgcctt
     2281 cgcgatcggc ttctcaagtt ccggggtgtc aatccatccc cacttcgacg actggaacgc
     2341 aatcagcccg caggctccct cgcgaacgaa atcctccatc tgttcgcgca cctgctccga
     2401 ggtcggcagc ccctgccccg agttcatcgg gcgaccgtcg aacgactgga aaacgccgat
     2461 gaatggaacg ccgggaaccc gttcccgcgc cttcgcgaga atccattgca cctcttcggc
     2521 agtgctttcc cgcatgtacc tgtccggttc cacggggtac cagtagatca tcatgatgtc
     2581 gcagatgccg ggtccaaagt tgagaaccga gcccgcatcg ccgtaccccg cgcatacggg
     2641 ctgccggatc tccgggtcgt acttccggac gatgcggtac atcgcgcgca gagcggaacg
     2701 cgcgtcgccg gggctgtcgt cgaggacgta ccacccgaga aggttgggag agtccttcac
     2761 ccgcagcacc tcctgctcac agtcctccgg ccagaagagt atcgtaccct cccggtgccg
     2821 cgcgggccgg gttccgttcc agccgcgtgc ggagccgatc aacgtgccgt tctccacgcg
     2881 ttcgtagcgg atcagttcgt cgtccagcgc gatgacgagc gagtccgaaa aatgatgcct
     2941 cggggcgaga aaaagcggga tttcagtctg ttccgccgaa atttcgttgc ggaggctgac
     3001 accgtggtac acgaactttt ccagcggata gagaaccctg atcccgcgtt gccgacaggc
     3061 cttcccctcg ggggctgcga ggtcaaggtg catctggccg gcaataatgg tgttcatccc
     3121 catcgaggcg gccttttcga tcgcttcaac ggtcggcacg gtatagctgc cgagaatcgg
     3181 cacgtgaccg atctgaaatg ccttgtgcag cagcatgtgg gtcgcgtcgc tcatcggtcg
     3241 gtgctccttc gagtgtcggt cggggactct tcgtaacgtg gcatgccttt ggccgcttga
     3301 cgtgaattat gtgccggacg gtgcgtgttc tcatggtacg gccgcttgag ggagcggtca
     3361 atgcggacgg acgcgctcac gcgcaaggcg ggtgattcgg atgcagaacg cggactttcc
     3421 attcgtgtag gcaacccggt cgttcggata ttcgcgggcc agccgcagtt tcagctcctc
     3481 atattccttt gccgtttccg ggtgcgcgat gaggtaatca cggaagagaa ggcgctccca
     3541 cagggcggaa tccgcctcca ccatgtggat atggtgcgtg cgcgaaccgg cgcggtcgcg
     3601 cttgatgaac cacgcgtaga agggcggtac gttgtcgccg aacaaaggcc gccagaagta
     3661 gtcgtacccc tgcgcctcaa gcaccggcgc gatgcgggtt ttcgtctcct ccagcgagcg
     3721 cacctccacc agcatgtcga tgatgggctt cgccgcgagg ccgggcaccg cggtgctgcc
     3781 gaaatgctcg atgcggcgta tgagcccgtc cggaaaactc gccagcaggt gcgccttttc
     3841 ctccgcgaac atgcgcggcc atgcggggtc atacggcacg atggccacat cctcgcgcac
     3901 cacccgggca atcctttgtt caagactttc caaattcatt actcctgagc cctggcccgg
     3961 agggacccgc tccgtcgtga ccttccggtt gtggttaatc ccgtcggaac gaggacccga
     4021 cagagcgggt ccctccaact gcctccgcat ggaaccttcc tcttgggaac cgccattcga
     4081 ccggttcaca cccgttctcg ggcggttctg tgagaaaccg ctcgctctac cacggcagga
     4141 cgttgatctc gccttgaaac ggccattcgt tcgggtttcc gcagagacgc ttgcggacag
     4201 ggttttggac aacataatcc catttgtcct cgtacctctc cgatctcctc agttgtgtat
     4261 cccagaacga cttttgccag attggttgct cgctccgccg tggccagcgt atcgaggcat
     4321 atgtcttcca acatttcacc cacacggaca actcggggtg gtcgggtccg gcaggcgaac
     4381 aaaacaggtg aacgtgatca ggcatgatca ggtatttgcc cacgagccag cctcgggtcg
     4441 cttcccacgc ctcacacagt gtctggtgcg cttccctggt ggccaggatc attttccgct
     4501 ccttcgcgca caccgtcagg aagacgataa tcggtccttc cgcgagcctg actgttggca
     4561 tatggaccgg tgatttcctg cgattcattg ggatttcttt caaggacgcc aagcgcgaca
     4621 ctgctgcttc atggtcgtaa ctgcccctga cgcgtctgga gggacccgct ccgtcgggtc
     4681 ccaaacgacc tgccccttgg gtcaaccggt cacgctctgc gcctgccagt caacaattcg
     4741 ctccctgagg gcggcaactt ccgccgcgta ttcacgcctg tccacaaggt tttccatctc
     4801 atacgggtcg gcggcgaggt cgtaaagcat cgtcggcgtg aactgctctc cctccaccac
     4861 cagcttcaga tctcccatac acaccatctt ccagtcgcgc atctcggaga acaccgggcc
     4921 gttgagttcc tgcgtttcgc cgcgggagag cggggcgaat gacctccccg gcagaccggg
     4981 cacctgcggg aggcccagcc agtcgagcgc agtggggaag aaatctatcc ccgaaacgag
     5041 ggctcctgtt cgcccaccgc cgatgccacc cggcacccgg acaatgagcg ggatgccgct
     5101 ggattcctca tacggcagcg acttgttctt caggccgtgg ctgccctgca tgtcgccgtg
     5161 gtccgacgtg aacacgacca cggtgtcctc cgccctcccc agccgctcga gttcggccag
     5221 aatccgcccc acgccggcgt ctatctgcgt gacgagagca tagtacaggc ggagatactc
     5281 gttcaggttg ttgccgtagc gctggaaatc agggcacgaa tcgggtggcc acgggctggg
     5341 tgggctccac gtgcgggtgt aggggtcgat ttcctggctg tttggcctcc gctggatggt
     5401 gacgcccgca tacatccgct cgaactccgg cgacggctgc accgggtagt gcggtgcctg
     5461 atacgagacg aacagcgcaa aggggtgcgg cgcgaggccc ctcagcttct caatggcgag
     5521 atcggtggtg acgtcagtcc ggtgccggtc gaagcagtgt tctcggtcgt ccctgtcgta
     5581 gaaacagacg ttgtggataa atccgttata acactgatac ccgatgaagt cggagaaccc
     5641 gccccgcagc tctttcggta tcgggccgtt gcccgcgccg ccgatatgcc atttcccgac
     5701 gtaacaggtt tcatacccgc cgctgttcag cgcatcggcc agcgtcacac accccgcagg
     5761 aatccgcgcc gcgttcttca tcgtcccggt ctgggaggaa tagagaccgg taaaaagatt
     5821 gatacggaat ggtgtgcata ccgggcagtt ggagtacgcc cgctcgaaca gcatcccgcg
     5881 ctgtgccagt gcgtcgaggt tcggggtgtg aatgtccggc gtgcccatgc agcgcagcgc
     5941 ataacggtgc atctgatcgg tgaagatgaa gagcaggttc acgatggctc ctttgcagaa
     6001 cgagaggact ggtcagaaaa atacggtgga ccggggtttc gtccatcccc gcgcgaatcg
     6061 gcaccacgcg ggggatggtg ccacggttgc cggagacgcg ggccacacct actttgtaaa
     6121 tgccgcatca ttgtccggca cgtaatgtgt gacgaagaga catttcgcag gagaccacgg
     6181 tggatttgct cgaccacggg ttctgggact acacaacgcc gtatgccggc ggaatggaag
     6241 cgtacaggcg ggatgactat ctccggcttg tggatgacat ggccgaagcc ggcatgaact
     6301 cgctcatgat ctgcatcaaa tggctgacga cggggtatcg gtcgcgcctg ccgtttctag
     6361 accagcttcc cggaaaccca gtgatcgagt cggataatgg gcttctgcgc gcagccatcg
     6421 cggcggcgca tgaccgcggc atgcgggtgt ggctgggcgc agtggtgagc atttttgtcg
     6481 tggaaaacta cggcggcgcg ccgtatgaaa cgtcggacag gcttcccctc gggtttccca
     6541 tcgtgccatt cggcctg
//
LOCUS       DAOTNG010000176         6550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10139_length_6550_cov_4.933025, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000176 DAOTNG010000000
VERSION     DAOTNG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10139_length_6550_cov_4.933025"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="P5179_09185"
     CDS             <1..444
                     /locus_tag="P5179_09185"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRS95430.1"
                     /translation="CERNACGSYGKSWGCPPHVGEIEELAARARSFAHGLLFQCITEI
                     EDSYDIEGMGRGAAHHKRVVRELAEIFRTRYGAPDVLVLGTGPCDFCEECTISTGQPC
                     RYPDRLISSAEAYGMNVKELVERCGFSYINGKNTVSYVGVILFNG"
     gene            complement(412..1386)
                     /locus_tag="P5179_09190"
     CDS             complement(412..1386)
                     /locus_tag="P5179_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS95431.1"
                     /translation="MELERSFSTHEVAELFGINVSTVKRWADSGDLPCMRTNGGHRRF
                     RLRDVVEFARSHELEPAGLRPMVEGLGDEAAGVGAEAPILNGNWVALREIVQRYALQG
                     DGTRLEELFGAVLVSGWHPAELCDHVIAPTMEYIGKQWAQNRLTVADEHLATHAITTA
                     LGRLPHMQEKSGRSPHRALCGTFAPDLHELACSCVALVLRFEGWDVTVLGAATPSDSF
                     VNCVERLRPDLVCVSATVIYDPVAFRTDCERLSLASLAVGNKYVIGGTALAEANLVCP
                     HGVARILKDMRGLVEYVRSEFPRNAARKTEGEEGDQSGSTVEKNDSDV"
     gene            1655..2296
                     /locus_tag="P5179_09195"
     CDS             1655..2296
                     /locus_tag="P5179_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS95432.1"
                     /translation="MQTSDRETRRREFEEIALPLQASLSRMALAMTRNPVEAADLVQE
                     TFYKAWRFFPAFRQGSDCKAWLTAILRNAYINRYRRIQRRPPQVEFDEWQEMDPSFRD
                     DISSFPAPDDDVALGDALSDEVLDALDQLPRHFRDVVVLSDVEGLSYQEVADFVGCPI
                     GTVRSRLFRARAMLRGLLGDYAAENGVGKSSDTVEDLIAELDEASMVEATVAE"
     gene            complement(2410..>2877)
                     /locus_tag="P5179_09200"
     CDS             complement(2410..>2877)
                     /locus_tag="P5179_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95433.1"
                     /translation="HSFDVMRYFCGPVKSVQAFMMKGKGRTIWSNTQVNLLFENNVVG
                     HLTGSYDAGGTYGLERAEVVGSEARFVLEDACEILTFYPRRSIEKEVYYHVGGMQSFG
                     NTFQSRIHRWLEQLTAGVKPEDVDGSGADGLAVQEIIEAAIKSWQTHSVVDLT"
     assembly_gap    2878..2977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3097..4017)
                     /locus_tag="P5179_09205"
     CDS             complement(3097..4017)
                     /locus_tag="P5179_09205"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRS95434.1"
                     /translation="MAFPGSGPIWFDGAFVPWEEAKIHVLSHVAHYGSGVFEGIRCYG
                     TKNGPAVFRMREHVRRMFDSAKIYRMPIPFTQEEIEAAILETIRRNGLDACYIRPLVF
                     RGYGQLGVSPLNCPVNVIIAVWEWGKYLGPEALEQGVNVCVASWNRPAANTFPSLAKA
                     SGNYLNSQLVKLEAMANGYDEGIVLDTSGYVAEGSGENIFVVRDSILFTPPTSSSLLP
                     GITRNSVITLARELNYTVREEQIPREALYIADEVFFTGTAAEITPIATIDKVPIGSGK
                     RGPVTKEIQDALFGILDGTIADRHGWLTPV"
     gene            complement(4216..4857)
                     /locus_tag="P5179_09210"
     CDS             complement(4216..4857)
                     /locus_tag="P5179_09210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS95435.1"
                     /translation="MGEIKDGQTLLFIGDSITDCGRRGPSYPLGDGYPSLFRDLMTAR
                     WPERNIRYINKGIGGNRVTDLQMRWEDDVMRYKPDWLSIKIGINDEHSFVADPNNGVS
                     PQRFREVYDSILQRTFSVWKPNLILISPFYISQDTVSGTHRTKILQLIPEYIAVVKDM
                     AAKYSAIYVPLHDIFQKHLEYRDADTFCPEPVHPYRSGHIIIARAIMHAIDES"
     gene            complement(4879..5427)
                     /locus_tag="P5179_09215"
     CDS             complement(4879..5427)
                     /locus_tag="P5179_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS95436.1"
                     /translation="MTRNALSAEDVIRALGLAPHPTEGGWYVETYRCPEEVNSTDLPA
                     RYRGNRSFSTAIYYLLTSETFSRLHRLASDEVFHFYLGDPVVMVHLLPDGTSRRVILG
                     HDIAAGQKPQVIVPRGTWQGAHLREGGKWALLGCTVSPGFDYRDYEEGSRDALVAMYP
                     DQRETIDRLTREDEGRSPRSLP"
     gene            complement(5441..5647)
                     /locus_tag="P5179_09220"
     CDS             complement(5441..5647)
                     /locus_tag="P5179_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRS95437.1"
                     /translation="MRKLYRSRTDKKIAGVCGGIAELCDIDPTIVRLIALLLILGYGA
                     GLIVYLVAWWLVPLAPEKPPDQQA"
     gene            complement(5658..6233)
                     /locus_tag="P5179_09225"
     CDS             complement(5658..6233)
                     /locus_tag="P5179_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRS95438.1"
                     /translation="MKRPCGSRNGVVLVFAIALLTTGTGACNSKNPPSREEVAMVRVT
                     SSAFSEGDSIPALYTCEGNDVSPPLKWSGVPAETKSIALTCTDPDAPRGTFVHWVLFN
                     LPPSTDGLPEGVTPDTVPQGAVNGTNDFPKLGYGGPCPPRGAPHRYYFTVYALDTVLA
                     LHPGARLRDLTAAMEGHILAEGSLMGRYARK"
BASE COUNT         1352 a         1939 c         1823 g         1336 t
ORIGIN      
        1 tgcgaacgga atgcctgcgg gtcgtacggc aagagctggg gctgccctcc ccacgtagga
       61 gaaatagagg aacttgccgc gcgggcgcgg tcgttcgccc acgggctgct cttccagtgc
      121 atcaccgaga tagaagactc gtacgacatt gaggggatgg ggcgcggcgc tgcacaccac
      181 aagcgcgtcg tgcgggaact ggctgaaatt ttccgcaccc ggtacggggc gccagacgtc
      241 ctcgttctcg gcacggggcc ctgtgatttc tgtgaggagt gcacgatctc cacaggccaa
      301 ccgtgccgat atccggatcg gttgatttcc tccgcggaag cgtacggaat gaatgtgaaa
      361 gaacttgtag agagatgcgg tttctcgtac atcaacggga agaacaccgt ttcatacgtc
      421 ggagtcattc ttttcaacgg ttgagcccga ttgatcgcct tcttctcctt cagtcttccg
      481 tgcggcgttt cgcggaaact cggaccggac atattccacc agcccgcgca tgtccttcag
      541 aatccttgca actccatgag ggcacacaag gttcgcttcc gcaagcgcgg ttccgcctat
      601 gacgtacttg ttcccgactg ccagggaggc gagggataac cgctcgcagt ccgtgcgaaa
      661 ggcaacaggg tcatagatga ctgtcgctga tacgcagacg agatcggggc gcaaccgttc
      721 aacgcagttc acaaacgaat ctgagggtgt agccgccccg agtaccgtca cgtcccaccc
      781 ttcgaaccga agaaccaacg caacgcacga gcacgccagt tcgtgaaggt ccggggcgaa
      841 ggtgccgcac aacgctcggt gtggggatct gccagacttc tcctgcatat gcggcagtct
      901 tcccagtgcc gtggtgatag cgtgagtggc gaggtgctca tcggcaaccg tgaggcgatt
      961 ctgtgcccat tgtttgccga tatattccat ggtcggcgca atgacatgat cacagagttc
     1021 cgcgggatgc caaccgctta ccagaaccgc cccgaagagc tcctcaagtc tcgtgccatc
     1081 gccctgtaac gcatagcgct ggacaatctc cctgagcgca acccagtttc cgttgagaat
     1141 cggagcctcg gcgcccacgc cggccgcttc atcgccgagc ccctccacca tcgggcgcag
     1201 tcctgcgggc tcaagttcgt gggagcgggc gaactccacc acatcgcgca agcggaagcg
     1261 ccggtgcccg ccgttggttc tcatacacgg cagatcaccg ctgtcggccc accttttcac
     1321 cgtcgacacg ttgatgccga acagttctgc tacctcgtga gtggaaaagc ttcgttcgag
     1381 ttccacggac cgcctccatg agcgttttga gcgtttttgg acgaggtaac accctgcgaa
     1441 tctctgcgga ttcgccgccg ttttcagggc cttttccgcc gtatgcgggc ggtgtggtac
     1501 gccataagta tatccttgtg cgtcaattat aattttaaca acaaaaatct taaaaaagta
     1561 gttgacacgg gtgtgcagac actgtatact ttaatcgtgc aaaccgttca acacctacgg
     1621 gcaacggagc atctgccagg aagcgaggaa cacaatgcaa acttccgacc gagaaaccag
     1681 gcgccgcgaa tttgaagaga tcgccttgcc tctccaggct tccctttccc gcatggccct
     1741 tgccatgacg cgaaatccgg ttgaggcagc ggatctcgta caagaaacgt tttacaaagc
     1801 ctggcggttc tttcccgcgt ttcggcaggg ctctgattgc aaagcgtggc tgacggcaat
     1861 actccgaaac gcgtacataa acaggtatcg aaggatacag agacgcccgc cacaggtgga
     1921 gtttgacgag tggcaggaga tggacccgag cttccgggac gacatttctt ccttcccggc
     1981 tcctgacgac gatgtggcgc tgggtgatgc gctcagtgac gaagttcttg atgcgctcga
     2041 ccaacttcca cggcacttcc gcgatgttgt cgtgctgtcc gacgttgagg ggttatccta
     2101 tcaggaagta gccgatttcg tcggatgccc catcggcacg gtacgatccc ggctgttccg
     2161 cgcccgggcg atgttgcgcg gactcctggg tgattacgct gcggaaaacg gggtgggaaa
     2221 gtcctccgat acggttgaag acctcatcgc cgaacttgat gaggcatcaa tggttgaggc
     2281 cactgtcgcg gaataagcac caaaacgttc gcaaacggaa agcggggagc agcttcgacg
     2341 ctgctccccg cgtgtttttt ctcaacggcg tggcgcacat tcgtgcacca aaacacctca
     2401 tcattcctgt caggtcagat caaccaccga atgagtctgc caggatttta tagcggcttc
     2461 gatgatctcc tgcaccgcca gcccgtcggc gcctgaacca tcgacgtctt ccggcttcac
     2521 gccggccgtc aactgctcca gccagcggtg gatgcgcgac tggaaggtat tcccgaagct
     2581 ctgcattccg ccaacgtggt aatacacttc cttctctatt gaacgccgcg gatagaaggt
     2641 aaggatttcg cacgcatcct cgaggacaaa ccgcgcctcc gatcctacca cttccgcgcg
     2701 ctcgagcccg taggttcccc ctgcgtcgta gcttcccgtc agatggccca caacgttgtt
     2761 ctcaaacagc agattgacct gcgtgttcga ccagatcgtg cggcccttgc ccttcatcat
     2821 aaaagcctgc acgcttttca cgggcccgca gaaatagcgc atcacgtcaa acgaatgnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc gcgtgggtgt tgccgatacc
     3001 gcccatgccg acgataccga ttttgagtgc catgacagtt tcctttgtga gagaagagtt
     3061 ctgaaaccag acctgtgatt cgttgtcgga gcaccgtcac accggagtta accacccgtg
     3121 gcggtctgct atcgtgccgt cgagaatccc gaacagcgcg tcctggattt ccttcgtaac
     3181 gggtcctctt ttcccggacc cgatcgggac cttgtcgata gttgcgatcg gcgttatctc
     3241 cgcggcagtg cctgtgaaga acacctcatc cgcgatgtaa agcgcctccc gcgggatttg
     3301 ctcctcgcgc acggtgtagt tcaactcccg cgcaagggta atgaccgaat tgcgggttat
     3361 tcccggcagg agggaggagc tggtgggagg cgtaaagagg atgctgtccc gcacgacgaa
     3421 aatgttctcg ccggatcctt ccgcaacata cccgctcgta tccagaacga ttccttcgtc
     3481 atacccgttg gccattgctt cgagtttcac aagctggctg ttcaggtaat tcccactcgc
     3541 tttcgcgagg ctcggaaacg tgttcgccgc cggccgattc cacgacgcga cacagacatt
     3601 gacgccctgc tccagcgcct cgggaccaag gtacttcccc cattcccaca cggcgataat
     3661 gacattcacc gggcaattga gggggctgac gcccagttgc ccgtaccccc ggaagacaag
     3721 cggccgaata tagcaggcat ccagtccgtt ccggcgaatc gtttccagaa tcgctgcttc
     3781 aatctcttcc tgcgtgaacg ggatcggcat ccggtaaatc ttcgcggagt cgaacatgcg
     3841 gcgtacgtgt tcacgcatcc ggaagacggc agggccgttt ttcgttccgt aacaacggat
     3901 gccttcgaaa actcctgagc cataatgtgc aacgtgggaa agaacatgga tttttgcttc
     3961 ttcccacgga acaaatgcgc cgtcaaacca gatcggcccc gaacccggaa acgccatcgt
     4021 cagatatctt cagggatcaa accttccctc ggacgccttt ctccgcccgc aaccttgctc
     4081 aaaacgacgt agccattcga aaactcgaca taacaggcgc gtacacgagg aagaaaagta
     4141 cggctgcgcg gcagaggggt caaccggcgt tcccggagca cttgcccacc agaaacgccg
     4201 gtctgaagga tattttcagg attcgtcaat cgcgtgcatg attgcccgcg caatgatgat
     4261 gtgtccactg cggtacgggt gcaccggctc agggcagaac gtgtcggcat cccggtactc
     4321 gaggtgcttc tggaagatat catgaagcgg gacgtagatg gcgctgtatt tcgcggccat
     4381 atctttcacc accgcaatgt attcggggat gagctggagg atcttcgtcc gatgagtgcc
     4441 gctcacggta tcctggctga tgtaaaacgg ggagatcagg atgagattgg gcttccacac
     4501 cgagaacgtg cgttgcagaa tgctgtcgta cacctcacgg aaccgctggg gcgacacacc
     4561 attgttcggg tccgcgacga agctgtgctc gtcgttgatt ccgattttga tggaaagcca
     4621 gtcgggcttg taccgcatga cgtcatcttc ccagcgcatc tgcagatcag tcacgcggtt
     4681 tccaccgatg cctttgttga tgtaccgaat gttccgttcc ggccagcgcg ccgtcatcag
     4741 gtcccgaaac agcgacggat atccgtcgcc gagggggtaa ctgggtcccc tgcgtccaca
     4801 gtcggtgatg ctgtcaccga tgaagagcag cgtctgtccg tctttgattt cgcccattgt
     4861 gaactccttt tgtgtgtgct acgggaggct gcgagggcta cgaccttcgt cctcgcgggt
     4921 aagtcggtcg atggtctctc tctggtccgg gtacatcgcg acgagcgcgt cgcgcgaccc
     4981 ttcttcgtaa tcccgatagt caaaacctgg ggaaaccgtg catcccagca aggcccattt
     5041 cccgccttcc cgcaaatggg cgccctgcca cgtcccgcgg ggaacgatca cctgcggctt
     5101 ttgtcccgct gcgatgtcgt gcccgagaat gaccctccgg ctggttccat ccgggagcag
     5161 gtgcaccatc actaccgggt ctcccagata gaagtggaac acctcatcgc tggcgaggcg
     5221 atgcagcctt gaaaaggtct cagaggtcaa aaggtagtag atcgccgtcg aaaaagaacg
     5281 gtttccgcgg taccgcgcag gaaggtccgt cgaattcacc tcttccgggc aacgataggt
     5341 ttcgacgtac cacccgcctt ccgtcgggtg cggcgccagt ccaagagcac gtatgacgtc
     5401 ctctgcggag agagcatttc gcgtcatcgg cacgacggta tcacgcctgc tggtccggcg
     5461 gcttttcggg agccaacgga accagccacc acgccacgag gtacacgatc aatccggcgc
     5521 catatccgag aatcaacagc agcgcgatga ggcggactat ggtgggatct atgtcacaaa
     5581 gctcggcgat tccgccacac acaccggcga tctttttgtc cgttcgcgag cggtacaatt
     5641 tgcgcatggc aacctcccta tttgcgagcg tatcgcccca taaggcttcc ttctgccaga
     5701 atgtgcccct ccatggcagc agtcagatca cgcaaccgag ccccggggtg cagggcaagc
     5761 actgtatcca gcgcgtagac ggtgaagtag taccgatgcg gggccccccg cggcggacat
     5821 ggtcccccgt agcccagttt ggggaaatcg tttgtgccgt tgacggcgcc ttgaggaacg
     5881 gtatccggag tcacgccttc tggaagcccg tcggttgagg gtggaaggtt gaacaggacc
     5941 cagtgaacga acgtgcccct gggagcatcg ggatccgtgc acgtgagggc gatgctcttc
     6001 gtttcggcag gtactcctga ccacttgagc ggcggtgaga cgtcgttgcc ctcgcacgta
     6061 tacagagcag ggatcgagtc tccttcgctg aaggctgagc ttgtcacacg caccatggca
     6121 acctcctctc gcgacggtgg gttcttgctg ttacacgcgc cggtcccggt tgtaagcaag
     6181 gcaatcgcga agacaagcac cacaccgttt ctggaaccgc aaggccgttt cattccattc
     6241 ccccggtcgt cggccctcac tgcaggcatc gagaatccca ttgagcgttt gcgagtgatt
     6301 tccacggaaa agatgagcaa aaacagcagc agaagcaacg tatgacacaa agtgagaaac
     6361 gccgaagggg aggcgcagga gtattggaag gcaggcctac cgcgcgataa ccactctgcg
     6421 ggccgtcgtc acgccctgaa aggacagacg caccacgtac acgccggagg ccacgggtct
     6481 ccccgaacta tcgctgccgt cccacgcaaa aaccccgttc gttcccgaga tcggaacgtg
     6541 cgtctcccgc
//
LOCUS       DAOTNG010000177         6476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10288_length_6476_cov_5.160411, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000177 DAOTNG010000000
VERSION     DAOTNG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10288_length_6476_cov_5.160411"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1985
                     /gene="nadB"
                     /locus_tag="P5179_09230"
     CDS             288..1985
                     /gene="nadB"
                     /locus_tag="P5179_09230"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRS95439.1"
                     /translation="MDVQTDFLVIGTGIAGLSFALQAAQLGSVAVVTKRKKDDTNTNR
                     AQGGIAAVLDQVDDFALHVNDTLVAGAGLCHEDVVTHIVREGPRIVRQLVDWGVQFTL
                     TSQGTLALGREGGHSHNRIVHAHDLTGAEIERALLAAIQQCPDVTIFENHVAVDLITE
                     HHLPGRRPARHVTCWGAYVFSAEENRVSRFLARRTVLATGGTGQAWRHTTNPDIATGD
                     GIAMAYRAGARVGNLEFMQFHPTTLYHPHERSFLISEAVRGHGAVLINDRGERFMDSV
                     HPMKSLAPRDIVARAIDREMKTTGKPCVYLDITHVDPDSVKDRFPNIYNKCLSLGIDM
                     TRQPIPVVPAAHYMCGGVVVDITSRTDILNLYACGEVALSGLHGANRLASNSLLEAVV
                     MANGAIEDIKQSGLARTPLPEIPAWDDSDVFDVEEWILLRHDIEEIRDLMWDYVGIVR
                     SDVRLRRAHHRLELIWKEVEEFYRRAKVNAELIELRNMACVAALVVRCAMERKESRGL
                     HFNTDHPMSDERFHHDTLLRARPIRRRKSMPPPHSINGKISEYHEIPGVSTPLRGEF"
     gene            2012..4360
                     /locus_tag="P5179_09235"
     CDS             2012..4360
                     /locus_tag="P5179_09235"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95440.1"
                     /translation="MSTRPTIRVVEPSNHHREKWPVTVSVPFPRSTVTSIGQVHLVDT
                     RDSEIPAQRRIMATWPDGSVKWMLLDFPVELEPRECRDISVEISDYEDGKEPEEGIRI
                     EWAGEELHVDTGVLRCRLAATQGGLLSEVIAHGITFSESPGTVRIAQPGGRMLDLSNG
                     PATLTIEEPGVQRAVIAMRGKHFDLEGNAALDYVLRMVVYANQAIVRWYYTIVNREPA
                     DAVPVSKIQMIQPFSLDTATCWAFVGTDRTRYSMPEGWVSMATDGLETRAHDGNRQSG
                     NLNTLRAEVQMEPFVVLGDATKMITVLPRWCHFLFPKAVHYYGRALRYDIWPDTAGVW
                     NFRRGMAKTHELVLAFSHPAEDYESAMPAVAPVLRPVIAAVDPHYIEETNAIPFFFAA
                     RGEAYPQLETHFARGFRGLARAYGMLHYGDANGVGYSAQGRGRHLKGKDAVEEVIWVN
                     NEYDMPYMAMLQFLRTGDRNVWLQTAEPHVWHMMDVDTVHSDPAHPINEGGQSIHLAN
                     HIGPPGYGVDPSHEWVEGLITYHLLTGLEHPREHALALGEHLLRWTEAHRADLNSDWM
                     ASRVTGWALIALTALFEFTRDERYRTCALAHGKGLHERVSGTTGHLTESVSYGFPYRA
                     GFMTNIAVIGLKRLWDISGDDLWKDLALKLIEDQWEHLSLPTGLLVYKELPENRYPSG
                     SATELESMAYAWQWTRDSVYLKRGIQLLRLFGPWRRDSASQGTFFTELPDGGLSQEIR
                     LFPRDSHSFSTFRYELPFLRVLHDTGILKQFEPPEIDLSGVT"
     gene            4384..5889
                     /locus_tag="P5179_09240"
     CDS             4384..5889
                     /locus_tag="P5179_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRS95441.1"
                     /translation="MSLLGIDVGTTGTKAVAFNHGGRPIASAYREYPLHSRQKGWMEV
                     DPHEVAAAIKDVISLVAAKTRHDPVSALSVSAMGEAAVPVARDGRFLAPVQVSLDERT
                     ASYPDWWRGRISNEDVFRITGHTIYSIFTLPRTQWIKEHWRSVYDQTWKFLCFQEFVF
                     YLLGTEPVTDYSQAARTMGFDVRAKQWSERLFEIAELDPGKFPVAVQSGTLVGKVADT
                     VANELGLPRGVKCVTGGHDQPCNALGAGILTGGSAVYGTGTVDCITPAFDHFVDSTDF
                     IQNNLACYPHVVPDKYCSVAYNSTGGNLLKWYRDTFADTEKRLAAEKGIDPYEVILSD
                     LPDGPSPVMVLPHFTVTGTPWFDTNSRGALLGLKLTTTKGEIVKALIEGTTFEMKLNL
                     EALRRSDVAVERIRSTGGGAKSRLWNQLKADMLGVPVATLQTSEGGSLGTAMLAGVAT
                     GVYGSLAEAASALIHEHEVFEPRPEVSTRYSERFAIYRQLYPTLREINHRL"
     gene            6001..6258
                     /gene="rpsT"
                     /locus_tag="P5179_09245"
     CDS             6001..6258
                     /gene="rpsT"
                     /locus_tag="P5179_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRS95442.1"
                     /translation="MANHKSAIKRIRTNREQQARNRQWMSRLRTVVKAVRKASTPEEA
                     KAAFEKAVPIIDSTARKGVIHKNAAARHKARLSNVLKALSA"
BASE COUNT         1433 a         1900 c         1876 g         1267 t
ORIGIN      
        1 tattgcatgt atctttgtac gtaagaccag tttaatcaat attttagtag aatatcgttc
       61 ggccaaccat gctcagaaca gggcaggaac tttttgcagg gtcggtcata aaagaaagct
      121 tgaacttcgt cggcccgcac aggataccct tttccgttcc cctttcgtac cggtcacccg
      181 ccttcgcctt gttcctgttt tgccccctcc ggtacattaa cgttaactcc tgccgcacgt
      241 cgtacgcgcg cttcgagtcc tcgcggcgcg aaccaacgag aagccacatg gatgttcaga
      301 ccgattttct ggtcatcgga accggaatcg caggcctttc cttcgcgctt caggctgcgc
      361 agctcggatc tgttgccgtc gtaacgaagc gaaaaaaaga cgatacaaac acaaaccgcg
      421 cgcagggtgg tatcgccgcg gtgctggacc aagtagacga tttcgcgttg cacgttaacg
      481 acactctcgt cgccggcgcg ggtttgtgcc acgaggacgt cgttacccac atcgtgcgcg
      541 aagggccgcg catcgtgcgc cagcttgttg actggggagt tcagttcacc ctgacttcac
      601 agggcacgct cgcgctgggg agagaaggag gacattccca taatcgtata gtccacgcac
      661 acgaccttac cggcgccgag atcgagcgcg cgctgctcgc ggcaatccaa caatgcccgg
      721 acgtgacgat cttcgaaaac cacgtcgccg tcgaccttat cacggaacac catctgccag
      781 gacggcgtcc cgcgcgtcac gtaacctgct ggggtgcata cgtgttcagc gcggaagaaa
      841 accgagtctc tcgctttctg gctcgcagaa ctgttctcgc aacgggaggc acgggacagg
      901 cgtggcgtca cacgacaaac cccgatattg caaccggtga cggaatcgca atggcctacc
      961 gcgcgggagc gcgagtggga aacctcgaat tcatgcaatt ccacccaacc actctgtacc
     1021 atccacacga acgctctttt ctaatcagcg aagccgttcg aggacacgga gcagtcctca
     1081 tcaacgaccg cggcgagcgg ttcatggact ctgtccaccc gatgaaaagc ctcgcgccac
     1141 gggatattgt cgcccgcgcg atcgacaggg aaatgaaaac aaccggcaaa ccatgcgtgt
     1201 atctggacat tacgcacgta gacccggaca gtgtcaaaga ccggttccca aacatttata
     1261 ataaatgcct gtcactgggc atcgacatga cccggcagcc cattccggtt gtccctgccg
     1321 cacattacat gtgcggtggt gttgtcgtcg atatcacctc gcggacggac attctcaacc
     1381 tctatgcctg cggggaggtc gctctttcag gcctgcacgg cgcaaatcgc cttgcctcga
     1441 acagcctcct cgaggcggta gtcatggcca acggcgcaat tgaggacatc aagcagagtg
     1501 gacttgcccg cactcctctt ccggagattc ccgcctggga tgacagtgac gtgttcgacg
     1561 tggaggagtg gatccttctt cggcatgata tcgaagagat ccgagacctg atgtgggatt
     1621 acgtcggcat tgtgcgttcc gacgtccgac ttcgccgcgc tcaccaccgt ctggaactga
     1681 tctggaagga agttgaggag ttctaccggc gggcaaaggt aaacgcggag ctcatcgagc
     1741 ttcgaaacat ggcgtgtgtg gcggcgcttg ttgttcgatg cgccatggaa cggaaggaaa
     1801 gtcgggggct gcacttcaac accgaccacc ccatgtcgga cgaaagattc caccatgata
     1861 cgctgttgcg cgcccgacca atcaggaggc gaaagtccat gccgcctccg cacagcatca
     1921 acggcaagat ctctgagtac catgagatcc cgggagtcag cacccctttg cgcggggaat
     1981 tctgagccag aaccttgggg agcctgaacg tatgtcgacc cgcccaacaa ttcgtgtcgt
     2041 cgagccatca aaccaccacc gggagaaatg gccggtcacg gtaagcgtgc cgttcccgcg
     2101 aagtacggta acctccatcg ggcaggttca tctggtggat acgcgcgatt cggagatacc
     2161 ggcgcagcgt cggatcatgg caacatggcc ggatggctcg gtgaagtgga tgttgctgga
     2221 tttcccggtg gaactcgagc cgcgggaatg ccgcgacatc tccgtggaaa tcagcgatta
     2281 cgaggatggg aaggagccgg aagaaggcat ccgcatcgag tgggcgggcg aggaactgca
     2341 tgtggatacg ggcgttctgc gttgcaggct cgcggcgacc cagggcggac tgctgagcga
     2401 ggtgatcgct cacggaatca ctttcagcga aagccccgga accgtacgca ttgcccagcc
     2461 cggtgggcgt atgctggacc tttcgaacgg accggcaact ctcacgattg aagagcccgg
     2521 cgttcaacgc gctgtcatcg ccatgcgcgg gaaacatttc gatctggaag ggaatgccgc
     2581 gctcgattac gtcctcagga tggtcgtgta tgccaatcag gccatcgtcc gatggtatta
     2641 caccatcgtg aacagggaac ctgctgatgc cgttccggtg agcaaaatcc agatgatcca
     2701 gcctttttcc ctggataccg caacctgctg ggcattcgtc ggcacggatc gcacgcgcta
     2761 ctccatgccc gaaggatggg tcagcatggc aaccgacggg ctggagaccc gggcgcacga
     2821 cgggaatcgt cagtccggga acctgaacac gcttcgggcc gaggttcaga tggagccttt
     2881 cgtcgtcctc ggtgacgcga cgaagatgat caccgtacta ccccggtggt gccattttct
     2941 cttcccgaaa gccgtccatt attacggtcg cgcgttgcgg tatgacatct ggccggatac
     3001 tgcaggcgtg tggaatttcc gccgcggaat ggcgaagaca catgagttgg tcctcgcgtt
     3061 ttcccacccg gcggaggatt acgagtccgc gatgcctgcg gtcgcacccg tgctccgccc
     3121 ggtgattgct gccgtggacc cgcactacat cgaggaaacg aacgccatac cgttcttctt
     3181 tgccgcgcgg ggagaagcct atccgcagct tgaaacgcat tttgcccgcg ggttccgcgg
     3241 cctggcgcgc gcctacggga tgcttcacta tggcgatgcg aacggagtgg ggtacagcgc
     3301 gcagggcagg ggaaggcatc tgaaaggcaa agatgccgtg gaagaggtga tctgggtcaa
     3361 caacgagtac gacatgccgt acatggcaat gttgcagttc ctgagaaccg gcgacagaaa
     3421 cgtgtggctt cagaccgcgg aaccacatgt atggcacatg atggacgtgg ataccgtcca
     3481 ctcggatccg gcgcacccga tcaacgaagg cggccaatcc atccatctcg caaaccatat
     3541 cgggccgccc ggttatggtg ttgatccaag ccatgaatgg gtggaaggac tcatcacata
     3601 ccatctcctc acaggactcg aacacccgcg ggaacacgct ctcgctctgg gagagcacct
     3661 tctccgctgg acggaagccc accgcgcgga tctgaattcc gactggatgg cctcgcgggt
     3721 tacgggctgg gccctgatag ccctgacggc gctctttgaa ttcacccgcg acgaacgcta
     3781 ccgcacctgc gcactcgccc acgggaaagg gctccacgaa cgggtgagcg gtaccacggg
     3841 ccacttgacg gaatcggtct cttacgggtt cccgtaccgt gcaggtttca tgacgaacat
     3901 cgccgtcatc gggctgaaac gtctctggga catctccggg gacgacctgt ggaaggatct
     3961 cgcactgaag ctcattgagg atcagtggga gcatctgagt cttcccacgg ggcttttggt
     4021 gtacaaggaa ctgccggaga atcgttaccc ttccggaagc gcaacggagc ttgagtccat
     4081 ggcatacgcg tggcaatgga caagggactc agtgtatttg aagcggggta tccagctcct
     4141 gaggctattc gggccgtgga ggcgcgattc tgcttcccag ggcacgttct tcaccgaatt
     4201 gccggatggt ggcctgtctc aggagatacg gctgttcccg cgggatagcc actcgttcag
     4261 cacattccgg tatgaacttc ctttcctgcg cgttctccac gacacaggaa tcctcaagca
     4321 gttcgaaccg ccggagatag acctctccgg agtaacgtga cgccgcgggg caaggaggcg
     4381 gacatgagcc ttttgggaat cgacgtcggc accacaggaa cgaaagcagt agcattcaac
     4441 cacggtggca gaccgatagc ctccgcatac agggagtacc cgctccactc tcggcagaaa
     4501 ggctggatgg aagtcgaccc ccacgaggtc gccgcggcta tcaaagacgt gatctccctc
     4561 gtggcggcaa aaacccggca tgatcctgtc agcgcacttt ccgtgtcggc catgggggag
     4621 gccgccgtgc ctgtcgcacg ggatggaaga ttcctcgcgc cggttcaggt cagcctggac
     4681 gaacgaacgg cctcgtaccc cgactggtgg cggggccgga tttccaatga agacgtgttc
     4741 cgcataaccg gccacactat ctactccatt ttcacgctgc cccgcacgca atggatcaaa
     4801 gagcactggc ggtcagtcta cgaccagacc tggaaattcc tctgcttcca ggagttcgtc
     4861 ttttaccttc tgggcacgga accggtcacc gattactccc aggcggctcg gaccatggga
     4921 ttcgacgttc gcgcaaagca atggtcggaa cggttgttcg aaatcgccga actcgatccc
     4981 ggtaagtttc cggtcgcggt tcagtcgggt actctggtgg gcaaagtagc ggataccgtg
     5041 gccaacgaac tggggttgcc ccgcggcgtg aaatgcgtga caggcggcca tgatcaaccc
     5101 tgcaatgcgc tgggcgcggg aattcttacc ggaggaagcg cggtgtacgg aacagggacc
     5161 gtggattgca tcacaccggc gttcgatcat tttgtggaca gcaccgattt catccagaac
     5221 aaccttgcct gttacccgca cgtcgtcccg gacaaatact gctccgtggc ctacaacagc
     5281 accggcggca atctgttgaa atggtaccgg gacacattcg ccgacacgga gaagcgcctt
     5341 gccgcggaaa aaggaattga tccctatgag gtcatcctca gcgatcttcc ggacgggcct
     5401 tcgccagtaa tggtgcttcc tcatttcacc gtaacaggaa caccatggtt cgacacgaat
     5461 tcccgcggcg ccctgcttgg cctcaaactg accacaacaa aaggggagat agttaaagcc
     5521 ctcatcgagg gcacgacgtt cgaaatgaag ctgaatctgg aagctttgcg aaggtcagat
     5581 gtcgcggtgg aaaggattcg gtctaccggc ggaggggcaa agtcccgtct gtggaaccag
     5641 ctcaaggcag atatgctcgg cgttcccgta gccacactgc aaacgtccga aggaggctcc
     5701 ctcggcacgg cgatgcttgc cggcgttgca acgggcgtgt acggatcact tgcggaagca
     5761 gcatccgcgc tgattcatga acacgaggta ttcgaacctc gtccggaagt ctccacgcgg
     5821 tacagtgagc gcttcgcgat ttaccgccag ctctacccga ctctcagaga gatcaatcac
     5881 cgattgtagc gggtttgttg aggtcgagat tgacaggggc catgccgttc cctattttta
     5941 tatgttcgtc attcttggtg aagaatcacg aggtgagcag acaagcataa ggagcccggc
     6001 gtggccaacc acaaaagcgc aatcaaaaga atccgcacga atcgcgaaca acaggcaaga
     6061 aaccgtcagt ggatgtcgag gcttcgcacc gtggtaaagg ctgtccgcaa agcctccacg
     6121 ccggaggagg cgaaagcagc ctttgaaaaa gcagtgccaa tcattgacag cactgcgcgc
     6181 aagggcgtca ttcacaagaa tgcggccgcc cggcacaagg ctcgtctcag caacgtgttg
     6241 aaagcacttt cggcataaac gagttttcca catatcggaa acgaagaagc cgcgtctcgg
     6301 acgacgcggc ttcttccatt ttcaccacac gccagacggc gaaacccgtc cctatttcag
     6361 ccacttttcg agccatggca acgcgtgcac gccattccac cggtgctcac cttcaaatac
     6421 atcgtggaaa acccggtcct ggacgccaaa aaccccgtag gacttccgaa tgatgt
//
LOCUS       DAOTNG010000178         6429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10378_length_6429_cov_4.479448, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000178 DAOTNG010000000
VERSION     DAOTNG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10378_length_6429_cov_4.479448"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="P5179_09250"
     CDS             <1..2285
                     /locus_tag="P5179_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRS95443.1"
                     /translation="HGFRLSGGTDMPPFATTGGFVARNGDGYGGPLGLADGLLSGIRC
                     VFASGDIVSSSQSGFNGPLDLHGFAVGSEGTLCVVTEATFRLEPAPPREVIVVALFRD
                     VVAATVAGVGLLKTGFLPDSADIIAAEAVRGALAGEAAVAAGAALLVSVSGGEEDVED
                     GAQWVSGVCHDFLAMQVIVTDNRQRDEIANFWRSAQRQFCSHHGGVLADFSCPLAALP
                     ELTGYALGQAREYGVVLSGMVRVASECLWMHGAAEQGDEAKEQAFLARFQEKALALKG
                     AALAAHGVGARRLGFLPSLHREADLDAMRRIDAVLDKRAVLSLSLPPARNAERRDIGA
                     RRKREEEVRALKAKTAEIANKASLGPLPESVLIPAPEILGLLVRSARERGIVLSINGF
                     PRGASLDLDTSGLNKVVLSDNASRVVVAEAGITLNDLASHLAANGLWFPGSPYVDGGI
                     RLGDFLAWETEGAYARGYGRMYTRVLGLEAVTGRGDVISWGGMVRMQHAGLRLSELCL
                     GKRNRYALITAVALRVAVIPATKQCVVASYDNVASASSFARLALRGGQGSSGHLAPRA
                     VALFNGPPGGGRSGKGVSVVVEFSGTSSSVAGHVNRATVLAAVEGADHITSHEDQACE
                     GLWRRIAERYSPVPNSADGENVKLVLTTPPDTLGFCLDHVLRVCSRRGRRCEFVADAL
                     AARADIVIPNGAAVAAEILRELDENLVETPARIEVFSLHEGLQEFLQNQGQTQLAEEL
                     KQQFDPSGILPSGLLPAIGR"
     gene            2302..2754
                     /locus_tag="P5179_09255"
     CDS             2302..2754
                     /locus_tag="P5179_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95444.1"
                     /translation="MSLLIDPKALRKWASNDLDVQKNQDGSYDFLFRYEGSSCRNGGR
                     AFPAEIRVCLSPADDGDWRIKDFAIIVPPAGREGWEATCIFGEIGKDSVRRMGTSVPF
                     RGQSLDEALASDVALNHAGCFCSPAHVNHKALLALWTIKWWLDDTAKM"
     gene            2861..5725
                     /gene="purL"
                     /locus_tag="P5179_09260"
     CDS             2861..5725
                     /gene="purL"
                     /locus_tag="P5179_09260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRS95445.1"
                     /translation="MYTIVISQKNPEMDAHARGTLADVRDHGLTGITRVEVDRIFRVE
                     GELSREAAELLANRLFVDPVVETWAVYGAGETPDRRGANHVLEVAYNPGVMDPVEGSV
                     LKASGDLGVALRAVRTAARYRFYGDATASEILEAAYALLANKTVNHVVMGNDDLPKPF
                     PAEEFRGPSHVELVGCDDEGLMAISRGRGLSLSVSEMRRIKEYFEHLGRNPTDIELET
                     LAQTWSEHCVHKTFKGRIEFDGEVIDNLLKQTIVKATQELGKPWCVSVFDDNAGVIEF
                     DEKNHICFKVETHNHPSALEPYGGAGTGIGGVIRDVLGTGLGAKPVANTDVFCFARPD
                     YPADEIPEGILHPRRIMRGVVAGVRDYGNRMGVPTVNGAVIFDDRYLGNPLVYCGTVG
                     LLPVGCERKTVLPGMKIVVVGGRTGRDGIHGATFSSAELGSDEENVWSNAVQIGNPIT
                     EKKVTDVLMMARDRGLYSAITDCGAGGLSSAVGEMGERTGAVVDLERAPLKYEGLSYT
                     EIWISEAQERMVLAVPPQHAEELLTLFESENVEATVIGEFTDNGRLVLRYHGVQVADL
                     DMRFLHKGVPQVRRNAVWRPPEERDPVFEEPASLNADLLALLSHPTIASKEWIIRQYD
                     HEVQGGSALKPLVGAENDGPSDATVIRPVLDSEKGVLLANGINPRYSLLDPYFMALAV
                     IDEAIRQVVAVGGNRSRTALLDNFCWGNPENPERLGELVRAARGCYEAAKAFETPFIS
                     GKDSFYNEYAVPSGTIAIPPTLLISAICVIDDVQKLVSMDLKEPGHFVYVVGLTRAEM
                     GGSHYWMLHDAVGRDVPKVDLATAPTAMDKMSAAIRSGCVRACHDCSEGGIGVAAAEM
                     AFSGGVGCEIDLGHLPVDSKHGRSDILLFSESPTRWICEVPPDRQKEFETIMEGTPFG
                     RVGQTTASRRMRVTGLSGDAVIDLALDEMKEAWQRTLREV"
     gene            5733..>6429
                     /locus_tag="P5179_09265"
     CDS             5733..>6429
                     /locus_tag="P5179_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95446.1"
                     /translation="MQVRVEDLEAYRDRLWCRLPSLRIHDEDEAVAYLERVPMCLAFG
                     GYALGVPTMWTGACGLRKPVFPKHSHHDPAVGLIWRAKETLVHSGAAYYGRLFLNKPS
                     FVARSWLATVLAGYPRVKLSQEAQAVSETLEESGPLPTRELGVRSGIRDRRVLTKALE
                     EAQTALRVTKVEERSDPFTYVWGTLSTLYAQEIDQAKGIPQDVARRKIAALWVNSLGL
                     TQAGRLARILGWDE"
BASE COUNT         1279 a         1662 c         2166 g         1322 t
ORIGIN      
        1 cacacggatt ccgactttct ggcgggacgg acatgcctcc gtttgccacg acaggaggat
       61 ttgtcgcgag aaacggagac ggttacggtg ggcccttagg cctcgcagat ggcctgttgt
      121 ccgggatccg gtgcgttttt gcatcgggtg acatcgtttc ttcttcgcaa tcgggtttca
      181 atgggccgct cgatttgcac gggttcgccg tggggtcgga aggaaccctc tgtgtcgtca
      241 ccgaggcaac gtttcgcctc gaacccgcgc cgccgagaga agtgattgtg gtagcgttgt
      301 ttcgggacgt ggttgccgca accgtcgcag gtgtgggtct gctgaagacg gggttcttgc
      361 cggacagcgc agacattatc gctgcggagg ccgttcgggg ggctctggca ggcgaagccg
      421 cagttgcggc aggcgcggcg ctgttggtgt ctgtctccgg tggcgaggag gatgtggaag
      481 acggtgcgca atgggtatcg ggtgtctgcc atgatttcct cgcgatgcag gttatcgtga
      541 ctgacaaccg gcagagggat gagattgcga atttctggcg ttccgcgcag cggcagtttt
      601 gctcgcacca tggtggtgtg cttgcggatt tttcctgtcc cctcgcggct cttcctgagc
      661 tgacgggata tgccttgggg caggcgaggg agtacggagt cgttctttct ggaatggtca
      721 gagtggcttc tgagtgtctc tggatgcatg gtgcagcaga acagggcgat gaggccaaag
      781 aacaggcttt tttggcgcgg tttcaggaaa aggcgttggc actgaaaggc gccgctctcg
      841 ccgcacacgg tgttggggcg agacgactgg gttttctgcc ctcgctgcac cgagaggccg
      901 atctggacgc gatgagacgt atcgatgcgg tgctcgacaa gcgcgccgtt ctgtctcttt
      961 ctctcccgcc ggcgcgaaac gcggaacggc gtgacattgg tgcacgcagg aagcgtgagg
     1021 aagaggtcag agcgctgaag gccaaaacgg cggagatcgc gaacaaggca tcgctcggcc
     1081 ctcttcccga gtcagtcctc atcccggctc cggagatact ggggttactg gtgaggtcag
     1141 cgagggaacg aggtatcgtg ctgtcaataa acggctttcc tcgcggcgct tccctcgacc
     1201 tcgacacttc gggtttgaat aaggtcgtgc tttccgacaa cgcaagcaga gttgttgtcg
     1261 cggaggcagg gattactctg aacgacctag ccagtcatct cgctgccaac ggactgtggt
     1321 tcccgggatc gccctacgtt gacggcggca tccgcctcgg ggattttctt gcctgggaga
     1381 ctgaaggcgc gtatgcgcga gggtacggga gaatgtatac aagggtcctc gggctggagg
     1441 cggttacggg ccgcggagac gtgatttcgt ggggcggaat ggtccgtatg cagcatgccg
     1501 gattgcgact ttctgaactc tgcctcggga agcgcaaccg gtacgcgctc atcactgcag
     1561 ttgcgttgcg agtcgcggtc attccggcca ccaaacagtg cgtggtcgcc tcttacgaca
     1621 atgtcgcgtc cgcgtcgagc ttcgctcgcc tcgcgctgcg gggagggcag gggagttcag
     1681 gacaccttgc cccccgcgca gtagcgcttt tcaatggtcc gccgggaggt ggtcgctcgg
     1741 gaaaaggcgt ctccgtggtg gtggagtttt cagggacctc ctcctcggtg gcgggacacg
     1801 tgaaccgtgc gacggtactg gcagccgtgg agggggcgga ccacatcact tcgcacgaag
     1861 accaggcgtg cgaaggcctc tggaggcgca tcgcagaacg gtacagcccc gtgccgaact
     1921 ccgcggacgg cgagaatgtg aagctggttc tgactacccc accggatacg ctcgggttct
     1981 gccttgacca cgtgttgcgt gtttgttcac gccgcgggcg caggtgtgag tttgttgccg
     2041 atgcgctggc tgcgcgagca gacatagtta ttccgaacgg ggcggcggtg gctgccgaaa
     2101 tactgcggga actggacgag aatctggtgg agacgcccgc aagaatcgag gtgttttcgt
     2161 tgcatgaggg cttgcaggaa ttcctgcaga accagggaca aactcaactg gcggaggaac
     2221 tcaaacagca gttcgacccg agcgggatac tgccttccgg ccttcttccc gcgatcggga
     2281 gataaccatc aggaatacgg catgagcctt ttgattgacc cgaaagccct ccgcaaatgg
     2341 gcttcaaacg atcttgatgt ccagaaaaac caggatgggt cttacgattt cctgtttcgt
     2401 tatgaaggct cttcgtgtcg aaacggcggt agagcatttc cggcggaaat ccgcgtctgt
     2461 ctctcacccg cagatgatgg agactggcgc atcaaagact tcgcgataat cgtgccgcca
     2521 gcggggcgcg aaggatggga ggctacgtgc attttcgggg aaatcggaaa ggacagcgtc
     2581 aggagaatgg ggacttcagt tccgtttcga ggacaaagtc tcgatgaagc cctcgcatcc
     2641 gatgtggcac tcaaccacgc tggatgcttc tgctcacccg cccacgtcaa ccacaaagcc
     2701 ctgctcgctc tctggacgat caaatggtgg ctggacgata cggccaaaat gtaagggccg
     2761 gacttgaaaa gcgcccgaga tcgcgttggc cgggcgtggt ggggaaccgt attttgggga
     2821 tattgggaat ttcagacatt tcgcctaaga gggcaacgcc gtgtatacga tcgtgataag
     2881 tcagaaaaac ccggaaatgg acgcgcatgc gagaggtacc ctcgcagacg tacgcgatca
     2941 cggactcacc gggatcaccc gtgtggaagt ggatcgaatt ttcagggtcg agggagagct
     3001 ttcgcgggag gctgccgaac ttctcgcgaa ccggctgttt gttgacccgg tggtggagac
     3061 gtgggcggtg tacggcgcgg gagagacccc tgacaggcga ggggccaacc acgtgcttga
     3121 ggtcgcctac aaccccggtg tcatggaccc agtggaagga tctgttctga aggcttccgg
     3181 tgacctcggt gttgcgctgc gtgccgtgcg cacagccgca agataccgtt tctacggaga
     3241 tgcgaccgcc tctgaaatac ttgaggccgc gtatgccctt ctcgcgaaca agaccgtgaa
     3301 ccatgtagtg atggggaatg atgacctccc caagccgttc cccgctgagg aattccgggg
     3361 tccctcccac gtagagctgg tcggctgcga tgatgaaggc ttgatggcga tcagtcgcgg
     3421 gcgcgggtta tccctgagcg tatccgaaat gcggcgcatc aaggaatact tcgaacacct
     3481 cggcaggaat cctactgaca tagaactgga aaccctggct cagacgtggt ccgagcactg
     3541 tgtgcacaag acgttcaaag ggagaataga attcgacggt gaagtaatcg acaacctgct
     3601 caaacagaca atcgtgaagg cgacccagga gcttgggaag ccctggtgcg tgtctgtgtt
     3661 tgatgacaac gctggagtga tcgagtttga cgagaagaac catatctgtt tcaaagtgga
     3721 aacccataac caccccagcg cgctggaacc ctatggcggc gccggtacgg gtatcggagg
     3781 ggtaatcagg gatgttctgg ggaccgggct cggggccaaa cctgtggcga acaccgatgt
     3841 gttttgtttt gcccggcccg attacccggc ggacgagatt ccggaaggca tactccatcc
     3901 acggcggatc atgcgcggcg ttgttgcggg tgtacgggat tacggcaaca gaatgggggt
     3961 ccccaccgtc aacggggctg tcatttttga tgatcgctat ctcggcaatc cgctcgtcta
     4021 ctgcggtacc gtcggtctgc tgcccgtcgg atgcgagcgg aaaacagtgc ttccgggaat
     4081 gaagatagtt gttgtcggcg gacgaaccgg gcgggacgga attcacggcg cgacgttcag
     4141 cagtgcagaa ctggggagtg acgaggagaa cgtctggtcg aatgctgttc agatcggcaa
     4201 ccccattacg gagaagaaag tcacggatgt actcatgatg gcacgtgaca gggggttgta
     4261 cagtgccatc actgactgcg gggcaggggg gttgtcttcc gcagtagggg aaatgggcga
     4321 gcggacgggt gcggtggtgg accttgaacg cgcaccgttg aagtacgagg ggctgagcta
     4381 caccgagata tggatttcgg aagctcagga acggatggta cttgcggtac ctccacagca
     4441 cgcggaggaa ctgctgaccc tgtttgagtc cgaaaacgtt gaagcgacgg tgatcggaga
     4501 gtttacggac aacggaaggc tggttctgcg gtaccacggt gttcaggttg cggacctgga
     4561 tatgaggttt ctgcacaaag gcgtgcctca ggttcgcagg aacgctgtgt ggcgaccgcc
     4621 ggaggagcgc gatccggttt ttgaagaacc cgcgtcgctg aatgcggatc tgctggcgct
     4681 tctttcgcat ccgacgatcg caagtaagga gtggatcatt cggcagtacg atcacgaggt
     4741 gcaaggcggg tcggcactga agccgctggt tggcgcggag aacgacggcc cttcggacgc
     4801 aacggtgatc cgtccggttc tcgacagcga aaaaggtgtg ctccttgcca acggtatcaa
     4861 cccgcggtac tcactcctgg atccatattt catggcgctt gccgttattg acgaggcgat
     4921 ccggcaggtt gtggcggtcg ggggcaaccg cagcagaaca gcgcttctgg acaatttctg
     4981 ctggggcaat ccggagaacc cggagcgcct cggggaactg gtacgggctg cgcggggatg
     5041 ttatgaggcg gcgaaggcat tcgagacgcc tttcataagc ggcaaggaca gtttctacaa
     5101 cgagtacgcc gttccttccg gcacgattgc gataccgccc acgctgttga tcagcgcaat
     5161 atgtgtgatt gacgacgtac aaaaactggt aagcatggac ctgaaggaac ccggccattt
     5221 cgtgtacgtg gtcgggctca cgcgtgcgga gatgggtggt tcgcattact ggatgctgca
     5281 tgatgcggtt ggccgtgatg tgccgaaggt tgacctcgcg accgcgccaa cagcgatgga
     5341 caagatgagc gccgcaattc ggtcgggatg cgtgcgggca tgccacgact gctctgaggg
     5401 aggtatcggt gtggcggcgg ccgagatggc gttctcgggc ggcgtgggct gtgaaatcga
     5461 tctggggcac ttgcccgtgg attcaaagca cgggcgatcg gatatcctgt tgttcagtga
     5521 gtcgcccacg aggtggatat gcgaggtgcc tcctgaccgg cagaaagagt tcgagacgat
     5581 tatggaaggc actccatttg gtcgtgtcgg gcagacgacg gcttcgcgga ggatgcgtgt
     5641 caccggactc tccggcgacg cggtgattga cctggcgctg gacgagatga aagaggcctg
     5701 gcagcgcacc ttgcgagagg tgtgaactcc ggatgcaggt ccgcgtggaa gatctggaag
     5761 cgtaccggga ccggctatgg tgccgccttc catcgctccg aatccacgat gaggatgagg
     5821 ccgtcgcgta tctggagcgt gttccgatgt gcctcgcttt cggcgggtat gcgctcggtg
     5881 tgcccacaat gtggacaggc gcatgtggcc tccgcaaacc agttttcccc aagcattccc
     5941 atcacgaccc ggcagtagga ttgatctggc gtgccaaaga gacgctggtc cattctggtg
     6001 cggcgtacta cgggcgtctg tttctcaaca aaccctcgtt cgttgccagg agctggcttg
     6061 ccacggtgct ggcagggtac ccgcgggtga agctttcaca agaagcacag gcagtttcgg
     6121 aaacgctgga agaatccggt cctcttccca cacgagaact gggcgttcgg tcggggattc
     6181 gtgatcgaag ggtccttaca aaggcacttg aggaggccca gacggcgttg cgggttacaa
     6241 aggtggagga acggagtgac ccgttcacgt acgtgtgggg cacgctgagc acgctctatg
     6301 cgcaggaaat tgatcaggcg aaggggattc cgcaggatgt cgcgcggagg aagatcgctg
     6361 cgttgtgggt gaattcgctt ggattgacgc aggcggggcg tctcgcaagg attctcggat
     6421 gggatgagc
//
LOCUS       DAOTNG010000179         6407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10421_length_6407_cov_4.584383, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000179 DAOTNG010000000
VERSION     DAOTNG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10421_length_6407_cov_4.584383"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..6214)
                     /locus_tag="P5179_09270"
     CDS             complement(116..6214)
                     /locus_tag="P5179_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS95447.1"
                     /translation="MLRAVLRAVLVLAVSLLGPVAHAETTVSGEITTTTWTKANSPYR
                     VAGDLYIPPGNTLTIEQGVDVIFDADALFTVEGTLTAVGTAADSIRFYPSGDGSWRGF
                     RVYSEGMVAAKPLSERAKAARVTQEGGASIEMAFARISGVQDADDEATGGAFEVVGAA
                     SGLTLTNCVIRDNTAYEGAGIAFVGGAGGMLSLVTFANNSASNQGGAIYLSDAFVQAI
                     SCTFVDNHAVNFGGAIAGIDYDTIGPLYRSAAGRSGQNGDGRGKRPSGAERPARPESP
                     VPRVARRAEDGEPSFIMLVLAQIRNNTSGDCGGAVYLQGTVGALMGSTVAQNSTTYDG
                     GGIHATNGFVQMQGCSVDSNSAGQDAGGVALYSQTMAGITDTRFRANQAEGDGGALNL
                     WDGAKLTIETSTFENNRSSDDGGAMTVYTGSTATVRECQFRGNAAGYDGGGIIVWDDC
                     ELTVENSLFEGNSSRSDGGGVTVYSNSTATVSGTTFVENTCDDGEGGGYGGAFEAWQN
                     VTVTMDSCTFTGNFAPESGGAVGIYDQSALTFRGCDFGGNHTETFGGALDVWYSDLQL
                     VDCALRNNAVTGPGYGGGVYAFNSRIAASNVLVAKNEADYGGGMYLDADASLALDRGT
                     VAHNVGRTGVGGLFLYGPGHSIANSIIWGNTGGNVYVTDQANVTIAYSDLPYEGVWPG
                     TGNINADPMFVDAAGGDFTLAEGSPCINAGDPAGDPDPDGSSPDMGWSPTGGGSIGGT
                     EVSGLLTTGTWTRANSPYRVTGDLELPEGEVLTIEPGVDVIFEEPAFFYVYGRLIAHG
                     TETDSIRFYPASAAAGWHGFEIESYGGMYRPLSTRAKATRAAYEGGASVEMSYVRISG
                     VRDIADYNDGGAFMVYGNTASVSVSNAIIRDNEAYDGGAANFVDGAMGQFTNVMFADN
                     YSEGDGGAVTFNDAFVSFEGCTFARNESYYAGGAVYVYDYDGGGLDKPAARAKADKHG
                     KADRRAVERPSRRADRLSARPARPTRPHRAAREDRSTKVSRDQRRALPGRAARAARPT
                     RQATDGELSYLYVEDCQFVDNYSDEDGGALYLHGVQGEFSQTSFNQNEASGEGGAVYS
                     DYSALSFTLSSFDGNYGPDEGGAVDAGYSTLSFDSCAVKNNVSDGYGGGFSLDGSVYL
                     TVNRSLFEGNSTDEGGGIVAYDDCHMVVQNSAFIGNSAWSYGGAMAVNSSSTLEVSNT
                     SFVDNTSEGTSGGIEVRDYSSASADGCTFTGNVGIDGGGGMGVYDDATVVVSNSTFRN
                     NGSAFSGGGVDVYQAGATIVECVFENCTAQSDADWTYGGALYANDAQLDVGRTLIAKS
                     TADHGGGVCLEMDVTATFTNCTIADNTGVEAGGGISLYDGVVTLNLLNTIVAWNEGGN
                     VLNPPEGLGQVVATYSDIFDEEVWPGERNISADPLFVDREAGDYRLAAGSPCIDAGDP
                     ESERDPDGTIADMGAFPTEKQGGPTPVSGTILTTLWKSTKSPYRVVGAINVPAGNVLT
                     IEAGVDVLFDADVDFIVDGKIIVTGTQTDSVRFLAGATEWGGFSILSPTDTSLFRYAR
                     FSGARANAGQYDGAGAMYINASKAILEHCVVSGNSGTYCGGGMGLDSDAMVWMSDCAV
                     VDNSCGHDGGGIYSARSTLQMQRCVVARNSSGGAGDGIQVGFDSVVDLVNCTVVANGG
                     AAFYWYPAGTLRLKNCIVWGNSSAGLITSATYSDIQGGVGGAGNINADPLFVNAAAGD
                     YRLSPGSPCIDAGDPASPRDPDLTRADMGAFPTGIPSKYGDVTGDGTVSAGDASLVLR
                     YVVGIVPSLPRPVAADVTANGSTSSYDAALILRKVLDDTYLFPAERGGMPGTKMAGTL
                     PRVLSWERAGSGWALRVNDPTGILAGDFQFALPDAREVNVAAGEYSAWRQEGSQLHLA
                     LVIEPESGAILVTVDGLSGAPEVLQASFNEGAIPVQAPRPLEFALAQNTPNPFNPSTV
                     IRYTLPENALVQLTVFSTTGQVIRTLVRAQVEAGSHQAVWDGRDDQGREVASGTYVCR
                     MAAGRYNSVMRMTLAR"
BASE COUNT         1177 a         2116 c         1858 g         1256 t
ORIGIN      
        1 gctggaggag gtctcgcgcg tgttgtaccc tcgcggcaaa gtcttatcgc aataattgaa
       61 aacaaccggc ggagcagcca tcagcgccgc tccgccggtt gttgcgcgta cctgcctatc
      121 tcgcgagtgt catcctcatc accgagttgt acctgcctgc tgccatgcgg cagacgtacg
      181 tgccgcttgc aacctccctg ccctgatcat cgcgcccatc ccagacagcc tggtgtgacc
      241 cggcttccac ctgtgcgcgc accagcgttc tgataacctg ccccgtcgta ctgaacaccg
      301 tcaactgcac gagagcgttt tcgggcagcg tgtaacggat gacggtgctc gggttgaacg
      361 ggttgggagt gttctgcgcg agcgcaaact caagcgggcg cggcgcctga accggaattg
      421 cgccctcgtt gaacgacgcc tgaagcacct cgggcgcacc cgaaaggccg tcaacggtca
      481 caagtatggc gcccgattcg ggctcgatca ccagcgcaag gtgaagctgt gaaccctcct
      541 gccgccacgc ggagtattcc cccgccgcca cgttcacctc gcgtgcatcg gggagtgcaa
      601 actggaaatc gcccgcaagg attcccgtcg gatcgtttac ccgcagtgcc cagccagacc
      661 cggcgcgttc ccagctcagc acccgcggca gggttccggc catttttgtt ccgggcatac
      721 cccctcgttc cgcggggaag agatacgtgt cgtccagtac tttgcgaaga atgagggctg
      781 cgtcgtaact gctcgttgaa ccgttggcag tgacgtcagc agccacgggg cggggcaacg
      841 agggaacaat ccccaccacg taccgaagca cgagcgatgc gtcgcccgct gataccgtgc
      901 cgtcgccggt gacgtcgccg tatttcgatg gtattcctgt cggaaacgcg cccatgtccg
      961 cccgcgtgag gtcaggatcc cgcggacttg ccggatcccc ggcatcgatg cagggcgatc
     1021 ccgggctcaa acggtaatcc ccggcggcgg cattcacaaa gagcggatcg gcgttgatgt
     1081 ttccggctcc tccaacgcca ccctggatgt cgctgtacgt cgcacttgtg atcagacctg
     1141 cgctggaatt gccccatacg atgcagttct tcaggcgaag cgtgcctgct ggataccagt
     1201 agaacgccgc tccgccgttt gcaaccaccg tgcagttcac caggtccacg actgaatcaa
     1261 atcctacctg aatgccgtcc ccggcgccgc cggagctgtt ccgcgcaacc acgcaccgct
     1321 gcatctgaag cgttgagcgg gcagagtaga tcccccctcc atcatggccg caggagttgt
     1381 ccacaaccgc gcaatcgctc atccagacca tggcgtcgct gtcaagcccc attccgccgc
     1441 cgcagtacgt tccggagttg ccgctcacga cgcagtgttc aagaattgct ttgctggcgt
     1501 ttatgtacat cgcgccggca ccgtcatatt gccctgcgtt tgcccgcgcg ccgctgaaac
     1561 gcgcataccg aaacagcgag gtatccgtgg ggctcaaaat gctgaagcca ccccattctg
     1621 tcgctccggc aaggaatcgt acgctgtcgg tttgagtgcc cgtcacgatg attttgccgt
     1681 ccacgatgaa gtcgacgtcc gcgtcgaaca gcacatccac tccggcctcg atcgtcagca
     1741 cgttccctgc gggaacattg atcgcaccga cgacgcggta cgggcttttt gttgatttcc
     1801 acagggtggt caggatggtc ccgctcaccg gtgtcgggcc gccctgcttc tcggtcggga
     1861 aggcccccat atccgcaatg gttccgtcgg ggtcacgttc actttctgga tcgccggcgt
     1921 ctatgcaggg cgaacccgcc gccagccgat agtcgcccgc ctccctgtca acaaacaagg
     1981 gatcggcgct gatgttcctc tctcccggcc agacctcttc gtcaaaaatg tcggaatagg
     2041 tggcaacaac ctgcccgagt ccttcgggcg gattgagcac gttcccgccc tcgttccacg
     2101 cgacgatggt gttgaggaga ttcagggtga ccacaccgtc gtagagggag atgccgccgc
     2161 ccgcctcaac accggtgttg tcggcgatgg tgcagttcgt gaaggtcgcg gttacatcca
     2221 tctcaaggca gacgccgccg ccatggtccg cggtgctctt cgcgatcagg gttcgaccca
     2281 cgtccagttg ggcgtcgttc gcgtacaacg cacccccgta ggtccaatct gcgtcgcttt
     2341 gggcagtgca gttttcgaac acgcactcga cgatggtggc accagcctga tagacatcga
     2401 cgccgccgcc ggagaaggca ctgccattgt tgcggaacgt gctgttgctg acaaccacgg
     2461 tcgcgtcgtc gtacaccccc atcccaccac cgccgtcaat tcccacgttc ccggtgaagg
     2521 tgcagccatc cgccgaggca ctggagtaat cccgtacctc gatgccgcca gaagtgcctt
     2581 cagaggtgtt atccacgaac gaggtattcg agacctcaag cgtgctgctg ctgttcactg
     2641 ccatcgcgcc gccgtagctc caggcggagt tgccgatgaa cgcactgttc tgaacgacca
     2701 tatggcagtc gtcgtaggca acaatcccac cgccttcgtc ggtcgagttg ccctcgaaaa
     2761 ggctgcgatt gaccgtgagg tagaccgacc catccagcga aaaaccgcca ccgtacccgt
     2821 cagaaacgtt gttcttgacg gcgcaactgt cgaatgacag cgtggagtac cccgcgtcca
     2881 ccgctccacc ctcgtccggg ccgtagttgc cgtcaaagga cgacagggtg aaggacagcg
     2941 ccgaataatc ggaatacact gcgccgcctt cgcccgaggc ctcgttctga ttgaacgacg
     3001 tttgcgagaa ctcaccctga acgccatgga ggtaaagcgc gccgccatcc tcgtcggagt
     3061 agttatccac gaactggcag tcttccacgt agagatacga gagttcccca tccgtggctt
     3121 gccgagttgg ccttgcggcc cttgcggccc ttccgggcag cgcgcgccgc tggtcacggg
     3181 aaaccttcgt ggaccgatct tcacgggctg cccgatgggg ccgggtcggc cgcgcggggc
     3241 gagccgagag acggtcagcc cttctcgagg gacgctcgac ggcgcgccga tctgccttgc
     3301 cgtgtttgtc cgctttcgca cgggccgcgg gtttgtcaag gccgccgccg tcgtaatcat
     3361 acacgtagac tgctccgccg gcgtaatagc tctcgttgcg tgcgaatgtg catccctcga
     3421 agctcacaaa cgcgtcgttg aacgtcaccg cgccgccgtc gccctcggag tagttgtctg
     3481 cgaacatgac attcgtgaac tgacccattg cgccatcgac aaagttggcg gcgccaccgt
     3541 cgtacgcctc gttatcccgg atgatcgcgt tgctgaccga aacggacgcg gtgttgccgt
     3601 acaccatgaa cgcgccaccg tcgttgtagt cggcaatgtc ccgaacgccg ctgatgcgca
     3661 cgtaggacat ctcaacggaa gcaccccctt cgtatgccgc acgcgtagcc tttgcgcgag
     3721 tggagagtgg ccggtacatc ccgccgtacg attcgatttc gaatccgtgc cagcctgctg
     3781 ccgccgacgc aggatagaac cggatactgt ccgtctccgt gccgtgagcg atcagtcgcc
     3841 cgtacacgta gaaaaaggcc ggctcctcga agatcacgtc gacgccaggc tcaatggtga
     3901 gcacctcccc ttcgggaagt tcgaggtcgc cggtgacacg gtacgggctg ttggcgcgcg
     3961 tccacgtgcc ggtcgtaagc aaacccgata cctcagtgcc gccgatgctg ccgccacctg
     4021 tcggtgacca ccccatgtca ggcgaagaac cgtccggatc aggatctccc gccggatcgc
     4081 ccgcgttgat gcaggggctc ccttccgcga gggtgaaatc ccctcccgcc gcgtctacga
     4141 acatcgggtc ggcgttgatg ttgcctgtgc ccggccagac accttcgtaa ggaaggtctg
     4201 agtacgcgat ggtcacgttc gcctgatcgg taacatagac attaccaccg gtgttgcccc
     4261 agataatact gttggcaatg gaatgcccgg ggccgtaaag gaagaggcct cccacacctg
     4321 ttcgccccac attgtgggca actgtgcctc tgtccagcgc gagggaagca tccgcgtcga
     4381 gatacattcc gccgccgtag tctgcttcgt tcttcgcgac cagcacatta gaggccgcaa
     4441 tccgcgaatt gaacgcatac acaccgccgc cgtacccggg ccccgtcacc gcgttgttgc
     4501 ggagcgcgca gtctacaagc tggaggtcac tgtaccacac gtcgagcgct ccgccgaatg
     4561 tctccgtgtg gttgccaccg aaatcgcaac cgcggaacgt aagggctgat tggtcgtaaa
     4621 tgccgaccgc acctcccgat tccggcgcaa agttgcccgt gaacgtgcag gaatccattg
     4681 tcacggtgac attctgccac gcctcaaatg cgccaccgta tcctcctccc tcaccgtcat
     4741 cacaggtatt ctccacaaac gtggttcctg aaacggtcgc ggtgctgttt gaatacacgg
     4801 tcacgccgcc gccatcactt ctgctcgaat tgccctcgaa gaggctgttt tccaccgtca
     4861 gttcgcagtc atcccagacg atgattccgc ctccatcgta cccggcggcg ttgccgcgga
     4921 actggcactc gcgcaccgtg gcggtgctgc cggtgtacac cgtcatcgcc ccgccgtcgt
     4981 cactggaccg gttgttctcg aacgtgctcg tttcgatagt gagcttggcg ccgtcccaaa
     5041 ggttgagcgc gccgccgtct ccttcggctt gattcgcgcg aaaacgcgtg tccgtgatgc
     5101 cggccatagt ctgcgagtag agcgcgacgc cgcctgcatc ctgacccgcg ctgttgctgt
     5161 caaccgagca cccttgcatc tgcacgaagc cgttggtcgc gtggatgcca ccgccgtcat
     5221 acgtcgtgct gttctgagcc actgtcgaac ccatgagtgc gccaacggtg ccctggagat
     5281 acaccgctcc gccgcaatcc ccggaggtgt tgttccgaat ctgcgccagc accagcatta
     5341 tgaaggacgg ctccccgtcc tcggcccggc gggcaactcg tggcacggga gattccggcc
     5401 ttgccggccg ttccgcaccg gaaggccgtt ttccgcgccc gtcgccattt tgcccggatc
     5461 tgccggcagc cgaccggtag agaggtccga ttgtgtcgta gtctatcccg gcaatggctc
     5521 cgccgaaatt cacggcgtgg ttatccacga acgtgcaact gatcgcctgc acgaacgcgt
     5581 cgctgaggta aatggctccg ccctggttac tggcgctgtt gttggcaaac gtgacgaggg
     5641 aaagcatccc gcccgcaccc cccacaaacg caatccctgc cccttcgtag gcggtgttat
     5701 cccgtatcac gcagttggtg agcgtgaggc cggatgcggc gccgaccact tcgaatgccc
     5761 cgccggtcgc ctcgtcgtcc gcgtcctgca cgccgctgat ccgcgcgaag gccatctcaa
     5821 tggaggcgcc gccttcctgc gtcacccgcg ccgctttcgc ccgctcagaa agcggtttcg
     5881 ctgcaaccat accttcggaa tacacccgga atccgcgcca cgaaccgtcg ccggaggggt
     5941 agaaccggat gctgtcagcg gccgtcccca cggcggtcag tgttccttct acggtgaaaa
     6001 gggcatcggc gtcgaagatg acatcaacac cctgctctat cgtgagcgta ttgccgggtg
     6061 ggatgtacag gtctccggcg acacggtacg ggctgtttgc cttggtccac gtggtagtgg
     6121 ttatttcacc ggaaacagtg gtttctgcgt gtgcgacagg cccgaggagc gacacggcaa
     6181 gaacgaggac agcgcgaagg actgcacgga gcatacaggg accccccatg tgcgttggct
     6241 gcgtgcccgg cagccagtct gtctgtgcgg tttgctggcg atggcgatat gaacatcatg
     6301 tatcggacag gatgtgacac cgaaaaaacg gactcagtct tccgtacgca acgcacatgg
     6361 ttggaccttc acccaccggc cgggtgacta tccgccttga ttcaaaa
//
LOCUS       DAOTNG010000180         6404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10428_length_6404_cov_4.787526, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000180 DAOTNG010000000
VERSION     DAOTNG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10428_length_6404_cov_4.787526"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1379)
                     /gene="rhaI"
                     /locus_tag="P5179_09275"
     CDS             complement(153..1379)
                     /gene="rhaI"
                     /locus_tag="P5179_09275"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRS95448.1"
                     /translation="MEMSRDLYENLADSLTKKGVDVARVEAAIKSFEIETPSWGYGNS
                     GTRFGVFKEPGAARTLHERLQDAAEVHRVTGVCPSVAIHIPWDKVDDFAAVRAEAAAL
                     GVRIGAVNPNVFQDKAYKYGSLTNVDPAIRQKAMDAILECVDIMTKVDSRILSLWFAD
                     GSNYPGQVDMRQRKRWMQEALAHIHGKLPVGARMLVEYKLFEPGFYHTDICDWGMSLV
                     MCQHSGVNAQVLVDLGHHAHGVNIEFIVASLLDEGMLGGFHFNNRKSADDDLTVGSIN
                     PFEVFLIFVEIVAAERSGLIKRPIAYMIDQSHIVKQKIEAMIQSVMNIQTAYARALLV
                     DWKALAHARSRNDAVDAERVIQDAYMTDVRPLLEKIRTDMGLEPDPILAFRNSGYLAK
                     IVEERKGDLKGAASWG"
     gene            complement(1500..3428)
                     /locus_tag="P5179_09280"
     CDS             complement(1500..3428)
                     /locus_tag="P5179_09280"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS95449.1"
                     /translation="MSKKPVRIVENGKSNAVIVVAKDLGHQATKAVDDLVRVVALMSG
                     SAIPVVEDGAVQKPGAQIHIGQTAFVREADLLPASLPVNGYRIAVLESGGISRLVITG
                     RASLGISHGVYSLLTQELGVMWGMPAAMFEDIPKRQTVEIGHIDRTEFPSFGFRVFSG
                     NDPDWIRRNRIDDGGRNLPYYGHGHNLFTIFPPEKYAKDHPEYYALLDGKRQWPERQN
                     GEGPQPCLTNPDVIRITIETVRAFYDANPEISTLSLCPNDSAKFCECPSCLALDEGME
                     RYRGRRMNSDSYFYFIEAVAKELMKTHPDRYVGTYAYWTTELPPRRIKKLPPNVVVYL
                     TQDSSQYFDPAYEARDRELLESWSNVAHHLAVYDYYGLGWFTPRVYPTIVKRILPFLP
                     KVNVKGFYNETYPYWAHVAPHLFLVTRLLWDVHEDPDAVLTRWYERIFRECASQVRSY
                     FETLEAGWMKAREGKWFQGLDWLAEQLHQWDPAIRDEAWRKINEAYEAAKNETTRQRI
                     AYIRQGNRLAYLLSKTLEETLSLGPKDKQLEPRLRAIFGRVAEANTLFHNFIEADPTY
                     GHAYYRGARATIQMQWWMGFLASLIGEVLSENQRLKKKLTDEEPTYREMIAAEAYPDV
                     PRRIREARVMFGRPFPGS"
     gene            complement(3722..6109)
                     /locus_tag="P5179_09285"
     CDS             complement(3722..6109)
                     /locus_tag="P5179_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95450.1"
                     /translation="MKRRLERFWFVVSVAILLVAMEKPVEARRIDLKNVTIVQPAAAE
                     SERTAVAARVLKEELEKRIGERVRLADAPPSRGVVFEISAQPSDSVSNGATLPERRPE
                     GFRVEIRTAGSNITTVQIVGADKRGALYGVGHVLRAVEWGPRGAWVPSEISAASAPKY
                     GFRGFQLGYRNTANSWDAWTPEQYEQYIRELVLFGANAIENIPIQDSDPSPHMKLSRE
                     EMNVRISRICQDYGIEYWVWVPADFDLADSAKKAEGLELFTRFCRRTPYLNGVFVPGG
                     DPGDNHPRLLLPHVRDLATILTQSHPNATVWMSLQGFNREKADYVYTYLQTYQPDWFG
                     GVINGPQSPLIQEDRARTPRKYAVRHYPDITHTVRCLYQTPQWDPAFAVTLGREPVNP
                     EPVYYALIHNAFAPFSHGFMAYSDGVHDDVNKVVFSVRAWDPEADVREILCDYTRFFF
                     GSAVSDLAADGILALERNWRGPVPTNGGIDATLALWQNLDRMAPENRGNWRWQMCQLR
                     ANYDAYVRQRLLYETSLENEANEWLLRAQVTGADSAAKMALRVLEKATTEPVRPELRA
                     RVEQLCDDLFRSIGLQTSVPKYQASGLERGAVLDFVDHPLNNRLWLQDQLAKVRSLTS
                     ETEKVRRLEEIARWENPGEGSFYDDIGHIAKQPHVVTGEGLSTDPMMIRNPNPGFWAW
                     DNNRSTWRPSLSTAMWSGHHQTGWPIALQYTNLDSGATYVLRVTGVGDAFPRANGERL
                     VPTLYGTRLGEFKEFPLPRHLTRGGELRITWDIPDEADVNWRNKSRVSEAWLLKR"
     gene            complement(6165..>6404)
                     /locus_tag="P5179_09290"
     CDS             complement(6165..>6404)
                     /locus_tag="P5179_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95451.1"
                     /translation="RYHVEIQTAGGWREFSTGTTIGQKKIDTRARVACEKLRVVIEEA
                     RGPVRIRSVGIYDIPDLSDESVWGHECKFCGADVV"
BASE COUNT         1338 a         2048 c         1607 g         1411 t
ORIGIN      
        1 gtcgaaggtg caacgtgcaa gcgctggagc agaccttgcg cgcgggtatc cggcaccggc
       61 acaggggtcg ccccccgcat caaattgccc acagcttgtt ctctatcatc agaccgatgc
      121 tgatcgcctc aagtgccgtg tgcggacata tatcagcccc agcttgcggc acccttaagg
      181 tcgcccttcc gctcttccac gatcttcgcc aggtaccctg agttgcgaaa cgcgagaatg
      241 ggatcgggtt ccagacccat atccgtacga atcttttcca gcagcgggcg cacatccgtc
      301 atgtaggcgt cctgtatcac ccgttcggca tccaccgcat cgttgcgcga gcgggcgtga
      361 gccagcgcct tccagtccac cagaagagca cgtgcatatg cggtctggat attcatcacc
      421 gactgaatca tcgcctcgat tttttgcttg acgatgtggc tctggtctat catgtaggca
      481 atcgggcgct tgatcaatcc cgagcgctcc gccgcaacaa tctcaacaaa aatcaggaac
      541 acctcaaacg ggttgatgga acccaccgta aggtcatcgt cagcgctttt gcgattgttg
      601 aagtggaacc cgccaagcat cccttcatcg agaagcgatg cgacgataaa ttcgatgttg
      661 acgccatgcg catggtgccc cagatccaca agaacctgag cgttcacccc gctgtgctgg
      721 cacattacca gagacatacc ccagtcgcag atgtcggtat ggtagaatcc aggctcaaag
      781 agcttgtact ccacgagcat tcgtgccccc acaggcagtt tcccgtgtat gtgcgccaac
      841 gcctcctgca tccagcgctt ccgctggcgc atgtccacct gtccggggta attcgatccg
      901 tcagcaaacc agagactcag aatccgtgaa tccaccttcg tcatgatatc cacacattcg
      961 agtatggcgt ccattgcctt ttgccggatc gccggatcaa cgttggtgag actcccgtac
     1021 ttgtacgcct tgtcctgaaa cacattgggg ttgaccgcgc cgattcgcac tccgagcgca
     1081 gccgcttcgg ctctgacggc agcaaagtcg tcaaccttat cccatggaat gtgaatcgca
     1141 accgacggac acacgcctgt gacgcggtga acctccgccg cgtcctgaag ccgttcgtgc
     1201 aaagttctcg cagcacccgg ctctttgaat acgccaaagc gcgtacccga attaccgtac
     1261 ccccacgacg gcgtctcgat ttcgaacgac tttatcgccg cctcaacccg cgcgacgtcg
     1321 acaccctttt tcgtcaacga atcggccaga ttctcgtaaa gatctcggct catttccata
     1381 cccctttcat cgcgcccaca ttaaaatgac cagacaaatg cgttccgcca accgcagcgc
     1441 gcattccctc accccgagct tcggggccag gctacccgcc acgtcacgat aaactcccat
     1501 caacttccgg gaaagggcct tccgaacatc acccttgcct ccctgatgcg ccgcgggaca
     1561 tccgggtacg cctccgccgc aatcatctcc cggtaggtcg gctcctcgtc ggtcagtttc
     1621 tttttgagac gctggttctc agacagcacc tcgccaatca aggacgcgag gaagcccatc
     1681 caccactgca tctgaatcgt cgccctggca ccgcggtaat acgcgtgacc ataggttgga
     1741 tccgcttcga tgaaattgtg aaacaacgta tttgcttcgg ccaccctgcc gaaaatggcc
     1801 cgcagacgtg gctccagttg cttgtccttc ggtcccaggg aaagcgtttc ctcgagcgtt
     1861 ttggaaagca ggtatgcaag gcggttgccc tgccggatgt aggctatccg ctgccgtgtc
     1921 gtctcgttct tggcggcttc atacgcttca ttgattttgc gccacgcttc atcacggatc
     1981 gcgggatccc actggtgcaa ttgctccgcc aaccagtcca ggccttggaa ccacttccct
     2041 tctcgcgctt tcatccaccc cgcttccagg gtctcgaaat aggaccgaac ctgggaggca
     2101 cattcccgga aaatccgttc ataccaccgg gtcagcacgg catccgggtc ttcgtgaacg
     2161 tcccaaagaa ggcgcgtcac cagaaacaaa tgcggagcaa catgagccca gtacggatac
     2221 gtttcgttgt agaacccttt gacattgact ttcggcagga acggcaaaat gcgttttaca
     2281 atcgtcggat agaccctcgg cgtgaaccat cccaggccgt aatagtcgta caccgccaga
     2341 tggtgggcga cgttcgacca ggattccagc aactcccggt cgcgcgcttc ataggcggga
     2401 tcaaagtact ggctcgaatc ctgcgtaagg tacacgacaa cgttcggcgg gagcttcttg
     2461 attcgccttg gaggcagttc cgtcgtccag tacgcgtagg tccccacgta tctgtctgga
     2521 tgtgtcttca tgagctcttt cgcaacagcc tcgatgaaat agaagtaact gtccgaattc
     2581 atgcgcctgc cacgatagcg ttccatccct tcgtcaagag cgagacagga cggacactcg
     2641 cagaacttcg cgctgtcatt cgggcagagg ctgagtgtgc tgatttccgg gttggcatcg
     2701 tagaacgccc gcaccgtctc gatcgtaatt ctgatcacat cggggttcgt gaggcatggt
     2761 tgcggcccct ctccattctg ccgctccggc cattgccttt tgccgtccag caacgcgtaa
     2821 tactccggat ggtctttcgc gtatttctcc ggcggaaaaa tcgtgaaaag attgtgtccg
     2881 tgcccgtaat atggcagatt ccttcccccg tcatctatgc gattccttcg aatccagtcg
     2941 ggatcgtttc cactgaaaac acggaaaccg aacgacggga actccgtccg gtcgatatga
     3001 cctatttcca cggtctgtcg cttcgggatg tcctcgaaca tcgccgccgg catcccccac
     3061 atgactccaa gttcctgcgt gagcagggag tacacgccgt gcgaaatgcc aagagaagcg
     3121 cgaccggtga taaccagtct cgaaatcccc cccgattcga gcaccgcgat ccgatatcca
     3181 ttcaccggaa gactagcggg caacaggtcc gcctcccgca caaacgccgt ttgtccgatg
     3241 tggatctgag cacccggctt ttgaacggct ccgtcttcca ccaccggaat cgccgagcca
     3301 ctcatcagcg caacaacgcg cacgagatcg tccaccgcct tcgtcgcctg atgcccgaga
     3361 tctttcgcca ccacgattac cgcgttcgat ttcccatttt ccacgatacg cacaggtttt
     3421 tttgacatca taaatctccc ggcatgcgtc cagatccctg ttgtgtttca gactcaccgc
     3481 aactcataac ggcaaaaaca cgcagaggca cccgccatcc tggttccact cagagaaaca
     3541 cctttgatat cctcggcaca agttcgcacc atttccaagg taatgattag ctgaattaca
     3601 cctcgcccag tccgtattat atgcgttact gcgcatattt atatcgtagc caattttcgg
     3661 tcgttttcag acaatcttgt tgccgccatt ctgggattcg cctcccagaa cggagcactt
     3721 ctcaacgctt caacaaccat gcttccgaaa cgcgcgactt gttcctccag ttgacgtcgg
     3781 cctcatccgg aatatcccag gttatccgaa gttccccacc ccttgtcagg tgccgcggaa
     3841 gcggaaattc cttgaactcc ccgagacggg ttccgtaaag agtcgggacg aggcgctcgc
     3901 cattcgcacg aggaaacgca tcacccacgc ccgtaacacg gagcacgtac gtcgcgcccg
     3961 aatccagatt cgtgtattgc agggcgatgg gccagcccgt ttggtggtga ccggaccaca
     4021 tcgccgttga aagggagggg cgccaggttg aacggttgtt gtcccacgcc cagaagccgg
     4081 ggttcgggtt cctgatcatc atcggatctg tagaaagccc ctcgcccgta accacgtgag
     4141 gctgtttcgc gatgtgacca atgtcgtcat aaaaactccc ctcaccaggg ttttcccacc
     4201 gagcgatttc ctccagtcgc cggaccttct cggtttccga ggtcagactc cgaaccttcg
     4261 cgagctggtc ttggagccac aaccggttgt tgagcggatg atccacgaaa tcgagcaccg
     4321 caccgcgctc caaaccggat gcctgatact tcgggacgct tgtctggagc ccgatcgagc
     4381 ggaaaaggtc atcacagagc tgctccacac gtgccctgag ttcgggtcga acaggttcgg
     4441 tggtcgcctt ttccaaaact ctcaatgcca tttttgcggc ggaatcagcg cccgttacct
     4501 gtgcacgcag aagccactcg ttcgcttcgt tttccaggct cgtttcatac aggagccttt
     4561 gtcggacgta cgcatcgtaa ttcgcccgca actgacacat ctgccagcgc cagttgcccc
     4621 tgttctccgg cgccattcga tccaggttct gccaaagcgc cagtgtggca tcaatacccc
     4681 cgtttgtcgg aacggggcca cgccagttcc tctccagcgc aaggatcccg tctgcggcga
     4741 ggtcagacac cgcggacccg aaaaagaagc gcgtgtagtc gcagagaatc tctctcacgt
     4801 ccgcttcggg gtcccatgcc cgcacgctga acaccacctt gttgacatcg tcgtgaacgc
     4861 cgtcggaata cgccatgaag ccatgcgaaa acggggcgaa cgcattgtga atcagcgcat
     4921 aatacaccgg ttcgggattc accggctctc ttccgagagt aaccgcgaac gccggatccc
     4981 attgaggcgt ctggtaaaga caccgaaccg tatgtgtgat gtctggataa tggcgaaccg
     5041 catatttcct tggcgttctg gcccgatcct cctggatcag agggctctgc gggccgttga
     5101 tcacgccgcc gaaccaatcc ggctggtaag tttgcaaata tgtgtagacg taatctgcct
     5161 tttcgcgatt gaacccctgc agcgacatcc acaccgtcgc attcgggtga gattgcgtca
     5221 gaatcgttgc gaggtcgcgc acgtggggaa gaagcaaccg cggatggttg tctcctggat
     5281 ctccgccggg cacaaacact ccgttcaggt acggcgtcct tcggcagaag cgcgtgaaca
     5341 gctccaatcc ttccgccttt ttcgccgagt ccgcgaggtc gaaatctgcc ggtacccaca
     5401 cccagtattc gatgccgtag tcctggcaga ttctgcttat tcgcacattc atttcttcgc
     5461 gcgaaagctt catgtgcggg ctcggatccg aatcctggat gggaatgttc tcgatggcgt
     5521 tggcaccaaa aagaaccagc tcgcggatgt actgctcata ttgttccggc gtccacgcgt
     5581 cccaactgtt cgcggtattc cggtacccga gttggaatcc cctgaatccg tatttcggtg
     5641 ccgatgccgc cgagatctcg cttggaaccc acgccccacg ggggccccat tccacggccc
     5701 gcagaacatg cccgacgcca tacagggccc ctcgtttgtc cgctcccacg atctgcaccg
     5761 tagtgatatt cgagccagca gttctgattt ccacccggaa tccttccgga cgccgttcgg
     5821 gcaatgtcgc accgttcgaa acactgtccg acggttgggc ggatatctcg aaaacgacgc
     5881 ccctggaagg tggcgcatcc gccaaccgca cacgctcgcc gatgcgcttc tcaagctcct
     5941 ctttcagaac ccgcgccgcc acggcagtac gctctgattc cgcggcagcc ggctgaacta
     6001 tcgtaacgtt tttcaggtca atccgtcgcg cttcaacggg tttctccatt gcgaccagga
     6061 gaatggcaac ggatacgaca aaccagaacc gttcgagcct cctcttcatc gttttcatcc
     6121 ccttatgact tcttgtgtgt ccgatgtact ccgcgcggcg cgattcagac aacatccgca
     6181 ccgcaaaact tacactcatg tccccaaacc gactcatcgc tcaagtccgg aatgtcgtag
     6241 atgccaacac tccgaatccg tacggggccg cgcgcctctt cgatgactac cctcaacttt
     6301 tcgcaggcca cccgtgcacg ggtgtctatc ttcttctgac cgatagtcgt tccagtggaa
     6361 aactcccgcc agccacccgc cgtctgaatt tccacgtggt aacg
//
LOCUS       DAOTNG010000181         6345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10558_length_6345_cov_5.113831, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000181 DAOTNG010000000
VERSION     DAOTNG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10558_length_6345_cov_5.113831"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1615)
                     /locus_tag="P5179_09295"
     CDS             complement(35..1615)
                     /locus_tag="P5179_09295"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRS95452.1"
                     /translation="MDDSPKDHCGVIAVYNLDKKSNRSVAPIVSSALLDLQHRGQLGA
                     GMTSFDPGRTHLLKTHKDTGSVHEVFQLGHPLEAKRLLEDYRGCAAIGHVRYATSGRD
                     QAEYAQPFERQHSRRWKWFSLAFNGNLANYSDLRDHLTKTRGYHMVYNVDTEVLVHHV
                     AYALRGEKKPFLVTVFSELTCDLDGAYNIVFINADGDLVAARSPLGIRPLCYGIKDGM
                     FVAASESVALTNMGIEDFVDVEPGEMVWVDGETRTVHVDRFTQPRAHAHCFFEWVYFA
                     HVTSVLEKHAVYDVRWRLGASLASREKLSMDADCVVVAVPDTAKPIGDAFGFTLGIPS
                     LEGIVRNRYVGRTFIEGEDRAGKVRRKYTLVTSVLRGRRVFLTEDSLVRATTLRGLVD
                     RARTQGGAKEVHLRIGSPPVIAPCFYGIDMSTLGELYAPKFLKPGYSDEELDRVCDEM
                     ARQLGADSLRYLPIGDLAACVGLPHDHLCTGCVTGRYPTKAGERIFAKEQARQSSMPA
                     GRSYEVAAVDHVAADEAG"
     gene            1890..3281
                     /gene="rimO"
                     /locus_tag="P5179_09300"
     CDS             1890..3281
                     /gene="rimO"
                     /locus_tag="P5179_09300"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRS95453.1"
                     /translation="MKKIPFQLDATHATGNPETRKGRTGRSVAMIPLGCAKAQVDAEQ
                     MLGALASAGYRIVHNPERAEVVVINTCGFIQAAKEESLQTIFESLKLKKTHGVRTVAI
                     TGCLAERYAAELIEEIPEADIVLPWSQENELVHRLDASLGIHRGEEPQRGERILLSPG
                     HWAYLRISEGCNHRCAFCSIPAIRGRHVSVPLADLVAETERLAARGVRELNVVAQDTT
                     LWGIDLYGKPSLPLLLKSIAKVPGIRWVRLLYTHPAHITDELARAFSESEVLVPYLDM
                     PIQHINDVVLKRMNRIVGRAQIERIIHEFRTKIPHMVLRTTVIVGLPYETDRRFRELV
                     DFVEETKFDRLGCFVFSPEEGTEAARMRNRAPQSVAAERQAIIMEAQRAISRNRNRDR
                     VGKEYEVLTERRTGNSAIARSYAEAPEVDGVIRVRGSRLPAPGEWSRVRITKAFDYDL
                     LAAPVGAPSGMSG"
     gene            3398..4537
                     /gene="dusB"
                     /locus_tag="P5179_09305"
     CDS             3398..4537
                     /gene="dusB"
                     /locus_tag="P5179_09305"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRS95454.1"
                     /translation="MNPGALDYQLDSAIVTGLAALPAVPPAASRVVRIGSVPIYGHAV
                     LAPMSGVCEQPFRWICRRAGASAVYTEFVSSDGLVRNNRHTERMLAFSEDERPVAAQL
                     FGSDPSVVFDAVQKIENMGVDIIDVNFGCPVRKVVKRDAGSALLKNPELLGRVLRAAV
                     DAARRVPVTAKIRSGWQSVNAREIATIVEANGAAAVAVHGRTQAMGYSGKADWGVIRA
                     VKETVTIPVIGNGDVFSPDAAVQMLNQTGCDLVMVARGAQGAPWLFAQINAAFAGELP
                     PNVEWAQRVEIAAMHLALLLRQRTELIAVRSFRKHLGFYTRGAPDSATFRREVFRCDS
                     ASEVLARMNEYAEFLAQFDGSVPKSRWGHTCNTQGPLTTSTEYDT"
     gene            4534..5283
                     /gene="larB"
                     /locus_tag="P5179_09310"
     CDS             4534..5283
                     /gene="larB"
                     /locus_tag="P5179_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRS95455.1"
                     /translation="MTPDRIRTLLEQVRDGSLSTEKAMETLRAFPFEDLGFAQLDHHR
                     ALRKGFPEVIFCESKTVEQVVAIADRIVSHGADLLATRVTAAQGEALCAAIPQARYNL
                     TARTVALRQSNQPPPTRGEILVVCAGTSDLPVAEEAIETAEAMGHPVSLLADVGVAGI
                     HRLLSAQDRLFAASVIIVVAGMEGALASVVGGMVGVPVIAVPTSVGYGAHFGGLAALL
                     GMLNSCATGVTVVNIDNGFGAAAAACIINRA"
     gene            complement(5463..6317)
                     /locus_tag="P5179_09315"
     CDS             complement(5463..6317)
                     /locus_tag="P5179_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS95456.1"
                     /translation="MAYRLPDFEYKARSRLVDLLEELGTCTATEAARMVFNSTSIPAS
                     LAESLLEALTRSDQRFGRTPGGQWFLARKPVRSALRGATFVVLDVETTGTQPPAHRIT
                     ELGAVRVRESQIVNEFCSLINPEREIPLEITRLTGISNSMVADKPTALQLLPDFAEWL
                     GDDVIVAHNAPFDRGFIDAQWREVFGMATGNRWLCTVRIARWLYPEIHSRSLGPLCRH
                     LGIPVGRQHRAGDDARATAMVLLREIDDLSARGINDFDALDAVVAPLNAHPAKKRCRR
                     RTVPVEQT"
BASE COUNT         1282 a         1835 c         1945 g         1283 t
ORIGIN      
        1 gcacccgcgg cgatccgggc agcgaaatcc gggttcaacc ggcttcgtcg gcagcaacgt
       61 ggtccacggc agccacttca taactgcgac cggccggcat gctgctctgg cgagcctgtt
      121 ccttcgcgaa aattcgttca ccggcctttg tcggatacct gccggtaacg catccggtgc
      181 acaggtgatc gtggggaaga ccaacgcacg ccgcgagatc gccaattgga aggtagcgaa
      241 gcgagtcggc cccgagctgg cgtgccatct cgtcgcacac tctgtccagc tcttcatccg
      301 agtaaccggg tttgaggaac ttcggggcat acaactcgcc aagggtggac atgtcaatac
      361 cgtagaagca tggcgcgatt accggtggcg aaccgattcg gaggtgcact tccttcgccc
      421 cgccctgtgt tcgtgcccgg tccaccaggc cgcggagtgt tgtcgcgcgc acaaggctgt
      481 cttccgtaag aaacacccta cggcctcgaa gcacgctggt cacgagggtg tacttgcgtc
      541 ttactttgcc ggcgcggtct tctccctcta tgaacgtgcg gccgacatac cggtttcgga
      601 cgattccttc gagcgacgga atgcccagcg tgaaaccgaa ggcatcaccg atgggcttcg
      661 ccgtgtctgg aacggcaacc actacgcaat cggcgtccat ggagagcttt tcgcgtgatg
      721 ccagagacgc gccgagtcgc cagcgaacgt cgtacaccgc gtgcttctcc agcaccgacg
      781 tcacgtgcgc gaaatatacc cattcaaaga aacagtgggc gtgggcacga ggttgcgtga
      841 aacggtccac gtgaaccgtc cgggtttccc cgtcaaccca gaccatctcc cccggctcga
      901 catccacgaa atcctctatt cccatgttcg taagcgcgac actttctgac gcggcaacaa
      961 acatcccgtc tttgatgcca tagcaaagcg gccttatccc gagcgggctt cgtgcagcaa
     1021 cgaggtctcc gtctgcgtta ataaacacga tattgtacgc gccgtcgaga tcgcacgtca
     1081 gttccgagaa caccgtaacg agaaagggct tcttttcacc tctcaacgcg tacgcaacgt
     1141 gatggacgag cacctcggtg tcaacgttgt agaccatgtg gtagccgcgc gttttcgtga
     1201 gatggtcgcg caagtcgctg tagttcgcga gattgccatt aaacgcgagc gagaaccatt
     1261 tccagcgccg gctgtgctgt cgctcgaagg gttgcgcata ctccgcctga tcccggccgc
     1321 ttgttgcata ccgcacgtgg ccgatcgcgg cgcagccgcg gtaatcctcc aacagacgct
     1381 tggcctcaag cgggtgaccc aactggaaca cctcgtggac ggagccggtg tccttgtgcg
     1441 tcttcagcag gtgggtacgc ccggggtcaa atgaagtcat tccggctccg agttggccgc
     1501 ggtgttggag gtccagcaac gcacttgaaa ctatgggcgc caccgagcgg ttgctctttt
     1561 tgtccaggtt gtagactgca atgacaccgc aatggtcttt cggggagtcg tccatcggtt
     1621 ctccggtctg atcgtggtga tgccgaggat gtgccgaggt ttcgttacgg aaaggcgtga
     1681 caacgagatc gcgcatcgta ttttccgctt cttatccgaa gatcgagcac acgtatacga
     1741 gggaacccgg gatacagaat atacggacga cgcccccgtg cccctacggc gccgtgggca
     1801 tcggttttct gttactggga agcggaacgt gcgtatcttg tacgaaccac acgtttgagt
     1861 ctgccgaagc ataatcacga gggctgccgt tgaaaaagat cccgttccag ttagacgcca
     1921 cacacgccac cggcaacccg gagacacgga agggtcggac cggccgttct gtcgcaatga
     1981 taccgctcgg gtgcgcaaag gcacaggtcg atgcagaaca aatgctgggc gcgctcgcct
     2041 cagccggtta caggatcgtg cacaatcccg aaagagccga agtggtagtc attaacacct
     2101 gcgggttcat tcaggcagcg aaagaagaga gtctccagac gattttcgag tccctgaagt
     2161 tgaagaagac acacggtgtt cgaacggtgg caatcacggg atgtctggcg gaacggtatg
     2221 cggcagaact catagaggaa atacccgaag ctgatatcgt gttgccgtgg tctcaggaga
     2281 atgaactcgt gcaccggctg gacgcttcgc tgggcatcca tcggggggag gagccgcagc
     2341 gtggcgaacg gattcttttg agcccgggtc actgggcata tctgcgcatt tctgagggat
     2401 gcaaccacag gtgcgcgttc tgctccatcc cggcgattcg tggaaggcac gtcagcgttc
     2461 ctctcgcaga tctggtcgcc gagacggaac gtctggcggc gcgtggtgtc cgagaattga
     2521 atgtggtggc gcaggatacc accctctggg gtatcgatct gtatggaaaa ccttcccttc
     2581 ctctgcttct gaaaagcata gcgaaggttc cgggaatacg gtgggtgcgc ctgctctaca
     2641 cacatcccgc tcatatcaca gacgaactgg cgcgcgcgtt ctccgagtca gaagtattgg
     2701 tgccgtatct cgacatgccg attcaacaca tcaacgacgt tgttctgaaa cggatgaacc
     2761 gcatcgtcgg tcgcgcacag attgaacgga tcatacacga attccgcaca aaaatcccgc
     2821 acatggtttt gcggactacg gtgattgtcg gccttcccta cgaaacggat cgacgtttcc
     2881 gagaactcgt cgattttgtc gaagagacga aattcgacag gctgggttgc tttgtcttct
     2941 cgcccgaaga gggaactgaa gcggcccgaa tgcgaaaccg cgcgcctcaa tccgtggcag
     3001 cggaacggca ggcgatcatt atggaagcac agcgcgcgat ttcacgcaac aggaacaggg
     3061 atcgggtcgg taaggaatac gaagtcctca cagagcgccg gacaggcaat tcagcgatcg
     3121 cgcgcagcta tgccgaagcg ccggaagttg acggcgtcat acgtgtgcgc ggctcacgtc
     3181 ttccggcgcc gggcgagtgg tcacgggtac gaattaccaa ggctttcgac tacgatctgc
     3241 tcgccgcgcc tgtgggtgcg ccttccggta tgagcgggtg agcctatcgc cgtggaaccg
     3301 atttccgttc cacatggttc gatacgatgt tggaggttgc gtctccgggg cattttcctg
     3361 ctctgcgagg ccaccgtctt gagcaggatt cgacgcgatg aatccgggag cgcttgacta
     3421 tcagctggat tccgccatcg tgaccggttt ggcggcgctg ccggctgttc ccccggcggc
     3481 atcaagggtc gtcaggatag gttccgttcc gatctatggg catgcggtac ttgctcccat
     3541 gtcaggcgtg tgtgaacaac cctttcgctg gatttgccgg cgcgcgggag cttcggcagt
     3601 ctataccgag tttgtgagct ccgacggtct tgttcgtaat aatcgccata cggaacggat
     3661 gctcgccttc tcggaagacg aacggccggt ggccgctcag ttgtttggct ccgacccctc
     3721 cgttgtattt gacgcagtgc agaaaattga gaacatgggt gtcgacatca ttgacgtgaa
     3781 cttcggatgc ccggtgagaa aggtggtgaa aagggacgca ggctcggcgc tccttaagaa
     3841 cccggagctc ctcggcaggg ttctccgagc tgccgtggat gcggcccgga gggtacccgt
     3901 tactgcaaag attcgttccg gctggcaatc ggtgaatgcg cgggaaatcg caacgatcgt
     3961 ggaagccaac ggcgccgcgg ctgtcgccgt gcacggccgc acccaggcca tggggtacag
     4021 cggaaaagca gactggggtg tcatccgcgc ggtgaaggaa accgtcacca tccccgtcat
     4081 cgggaacgga gacgtctttt cgcccgatgc ggcggtccag atgctgaatc agacgggttg
     4141 cgaccttgtg atggtggccc gtggcgcaca gggcgctccc tggcttttcg cgcaaatcaa
     4201 cgccgcgttc gcgggggagt taccaccgaa cgtagaatgg gcacagcggg tggagatcgc
     4261 agccatgcac ctcgctctcc tgcttaggca gcgtacggaa ctgatcgcgg taaggagttt
     4321 tcgcaagcac ctcggtttct acacccgggg agcacccgat tccgcgacct tccgtcgtga
     4381 agtgttccgg tgtgattccg cgagtgaagt tctggctaga atgaacgagt acgcggaatt
     4441 tctcgctcag ttcgacggga gcgtgccgaa gtcgcgatgg ggtcacacgt gtaatacgca
     4501 gggtccttta accacctcca cggagtatga tacatgacac cggaccgtat ccgaaccctc
     4561 cttgaacaag tgagagacgg ttccttgagc accgaaaaag ccatggaaac cctccgggct
     4621 ttcccattcg aagacctcgg tttcgcgcag ctggaccacc acagagcgtt gcgaaaggga
     4681 ttccccgagg tcattttttg cgaatccaag actgtcgaac aggttgtggc tatcgcggac
     4741 cggattgtct ctcacggggc ggatcttctg gctactcgtg tgaccgccgc gcaaggggag
     4801 gcattgtgcg cggcaattcc tcaggcgcgg tacaacctta ccgctcggac ggtggctctt
     4861 cggcaatcga accagccacc ccccacgcgg ggagagattc tggttgtatg cgccggcacc
     4921 agcgatctgc ccgtcgcgga ggaggcgatc gaaaccgcgg aggcgatggg acaccccgtg
     4981 tcgctgctcg cggacgtggg tgtcgcaggt attcaccggt tgctcagcgc gcaggatcgg
     5041 ctgttcgccg ccagcgtgat cattgtcgtc gctggaatgg aaggtgcact ggcaagcgtc
     5101 gtgggcggta tggtgggcgt tccggtgatt gccgtcccga cctcagtcgg gtacggagct
     5161 catttcgggg gactcgcagc attgctgggc atgttgaaca gctgcgcgac gggagtgaca
     5221 gtggtaaaca tcgacaacgg gttcggcgcg gccgctgccg cctgtatcat caaccgggca
     5281 tgagatgtga gagccgggtc catccctggt ccgggtgaaa tcgcgcggcg ttggactggt
     5341 aaccaggcag actgtttcgc caacactgct accggagcga accgcaaggc aattgctgca
     5401 cgtggagcag cgcagccgca gtgacaccag tcctggtgac gtacaagaac cgcaggaaat
     5461 cgtcaggttt gctcaaccgg aaccgtccgc cgtcggcacc gttttttcgc ggggtgcgca
     5521 ttgaggggag ccactaccgc gtccagagca tcaaagtcat tgattcctcg ggcagaaagg
     5581 tcgtcaattt cccgcagaag caccatcgcc gtcgcccggg cgtcatctcc cgcgcggtgc
     5641 tgtcgtccga cgggaatgcc gagatggcgg cacaacgggc caaggctcct gctgtggatt
     5701 tccgggtaca accagcgggc aattcggacc gtacacagcc accgattccc tgtcgccatc
     5761 ccgaacacct cgcgccactg cgcgtcgatg aagccgcggt caaatggcgc attgtgtgcc
     5821 acgattacgt cgtctccaag ccactcggcg aagtcgggca gtagttgcag tgcggtgggt
     5881 ttgtcggcca ccatcgagtt tgatatcccg gtcagccggg tgatttccaa tggaatctcc
     5941 cgttcgggat tgatcaggct gcagaactcg ttcacgatct ggctttccct gacacgaacg
     6001 gcaccgagtt cagtaattcg gtgagccggc ggttgggttc cggttgtttc cacgtcaagc
     6061 acaacgaagg tcgcccctcg caacgcactg cgaacaggct tccgagcaag gaaccactgc
     6121 ccgcccggag ttcgtccgaa gcgttgatcc gatcttgtga gcgcttccag caacgactcg
     6181 gcaagcgagg ccggaatgga ggttgagttg aacaccattc gagccgcttc ggtcgccgtg
     6241 cacgtgccga gttcctcaag aaggtcgacc agacgggagc gcgccttgta ctcaaagtcc
     6301 ggcagacggt aggccaagga agagcccctt tgtggcgctc aagtg
//
LOCUS       DAOTNG010000182         6276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10700_length_6276_cov_5.294165, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000182 DAOTNG010000000
VERSION     DAOTNG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10700_length_6276_cov_5.294165"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="P5179_09320"
     CDS             complement(<1..671)
                     /locus_tag="P5179_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95457.1"
                     /translation="MAEGSAPIIETRDLTRKFGQFVAVDRVTLRVNRGEIFGFLGPNG
                     AGKSTTIRMLCGILAPTSGTALVGGFDVAREPDRVKEHIGYMSQRFSLYPDLTVAENI
                     RFYGGIYGLSAQQLRERGAWAVEMAGLKGREDHLTRELAGGWKQRLALGCAILHEPPI
                     LFLDEPTSGVDPISRRRFWDLIYELAGHGTTVFVTTHFLDEAEHCDRLVLIYRGRVIA
                     LGTPE"
     gene            complement(686..2089)
                     /locus_tag="P5179_09325"
     CDS             complement(686..2089)
                     /locus_tag="P5179_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS95458.1"
                     /translation="MRRTRWYGVSLAAIGTGLGLWSSGVAQTEAAGNALRLTVARARE
                     LAMTQNAGIRASAQSVISATAQKNEARAARLPGVSLSARYARLSDVPDPVIMTPSLTI
                     PTGPGTSITTPSTQVSLGPSVLNSYNLTATAQVPLFTGFRLENREKAAEYAAKAAGED
                     FSASQSEVGNRVEQVYWRLHSARATERVISESVRLAEAHLEDVRRLHAAGMATADDIL
                     AVQVRLAEAKLRQIQAENASELVRATLCNLLSVPVTTEIALVDSPSVVLPTVPEQDAL
                     VSAAIANRPELRALAFRKQAMERGTAVAEGGKLPSVMFQTNYEVSNPNQRYFPTRDAW
                     HATWNAGIGVTWTAWDWGMVGQQAARARAEERQVDERLNQVRDAITLQVVQARLAVIE
                     GNRRVAVAEEGVRSAEAYHRNMRARYSAGTATNTEVLDAQILLERARLDLVQARTDQR
                     TAAANLAYVVGAERTQD"
     gene            complement(2086..3075)
                     /locus_tag="P5179_09330"
     CDS             complement(2086..3075)
                     /locus_tag="P5179_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95459.1"
                     /translation="MTDHVARAGGTMPAAALETRELTRFFGTTHAVNGVSFSVGRGEM
                     LALVGPDGAGKTTTIRMLTGVLKPTSGTATVLGHDLVRERDAVKRSIGYLSQVFSLYG
                     DLTVDENIEFFAQIHGVRRFQKRREELLSFTRLAPFRRRLAGNLSGGMKQKLALACTL
                     IHSPGLIFLDEPTTGVDPVSRRDFWIILSDLLREGITVVLATPYLDEAERCHTVALMH
                     EGRIIRRDTPAAIVETFGKRLLEVVCEDARAAYRAMKQAPEWEEAQMFGSRIHVPVED
                     VVAGTQRLQEALEEMQFRVASIREIPPSLEDAFISEIRHSEGPVNPSEGGTAA"
     gene            complement(3072..4058)
                     /locus_tag="P5179_09335"
     CDS             complement(3072..4058)
                     /locus_tag="P5179_09335"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS95460.1"
                     /translation="MGKMAWVAPVLAIGLGACSRGASDGDITASGVIECTEINIATKV
                     PGILREVLPREGDLLDSGQVVAVIDHSDLDWQKSQAEAQLRVARANLQLALNGPQRED
                     IAQAEAAEQQASVALEAARLDLERVSQLAATGTAPQKQLDDARTRVRSAEAAQAMARA
                     TLAKLRAGTRSEQIAAARAQVEQAEAALSAVSQRIADCVVRSPRKGYVTQRIAEPGEM
                     VPSGGTIVTLSELDPVWLKVYLTEVELAKVRLGGTAEVTLDGEPDHPRSGRVSWISPK
                     AEFTPKNVQTKEDRVKLVFAVKISLQNPDGFFKPGLPADAAFRAGDGAGAPQ"
     gene            complement(4037..4720)
                     /locus_tag="P5179_09340"
     CDS             complement(4037..4720)
                     /locus_tag="P5179_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS95461.1"
                     /translation="MNRDSDARQRILDAATEVLVDKGLSGARMQEIADRAQINKAMLH
                     YYFTSKEKLYETVVSAAVQGIMERVQGVLKGEDVPTEKRFLMLVDTYLDSLSKKPHVP
                     RLIMMDLLAGGEIVMRALPQAQHRASFVGAKPALDLIRRGIHEGVIHEIDPKHTLISV
                     LGMVAFSFLAEPLLSSMLQIEDRKAGQYFADRRRNVMEILQHGVLKEQDSTRVSTIPR
                     EDKWEKWLG"
     gene            complement(4829..5437)
                     /locus_tag="P5179_09345"
     CDS             complement(4829..5437)
                     /locus_tag="P5179_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95462.1"
                     /translation="MKLFAALTGASIALAAGILSWWSAAILSILRWVPLTDSTIAWAA
                     CSGLAAGVFLAWVWYRRNAGRVFSIPMGHLAVLYVIWSVFSFAFFMGFPFGAFLLGWL
                     AAVYLGRRAAYERHGVECGVRKQVRFIGFVLGVECLAAGFFGALGDPHTWRVVLSLFG
                     AEMLAGSGILSLLTVIGVCAVLVSVQIAVAGWIAGRASRWGS"
     gene            5679..>6276
                     /gene="guaA"
                     /locus_tag="P5179_09350"
     CDS             5679..>6276
                     /gene="guaA"
                     /locus_tag="P5179_09350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRS95463.1"
                     /translation="MNVPVQERIVVLDFGGQYAHLIANRVRRLRVFSEIMLPSEPPAN
                     LEGVRGLILSGGPSSVHSPDQPPFDARWLHTGLPVLGLCYGHQLLAKELGGEVRRGDR
                     QEYGIAELHITDHDAIFEGLEPVERVWMSHGDSVTRVPDGFEVLGGTADCPIAAMGDV
                     QRRMFGFQFHPEVTHSPHGQKMLDNFLSLCGCARTWTME"
BASE COUNT         1179 a         2198 c         1711 g         1188 t
ORIGIN      
        1 tcttccggag tccccagcgc gataacgcgg ccccggtaga tcagcaccag acggtcgcaa
       61 tgttccgcct catcaagaaa gtgggtcgtc acgaacaccg ttgtgccatg tcccgcaagt
      121 tcgtagatca ggtcccagaa ccgacggcgg gaaatcgggt ccacccccga cgtcggctcg
      181 tccagaaaca gtatcggcgg ctcgtgcagt atcgcgcatc cgagcgcaag gcgctgcttc
      241 cacccccctg caagctctcg cgtcaggtgg tcttcccggc ccttaagacc ggccatttcc
      301 accgcccatg cccctcgctc ccgcaactgc tgcgccgaaa gcccgtagat gcctccataa
      361 aaacggatat tttccgcaac cgtcaggtcc ggatagagcg aaaaccgctg cgacatgtag
      421 ccgatgtgct ccttcacccg gtcaggctca cgtgcaacat cgaaccctcc caccagcgct
      481 gttcccgaag tgggcgcaag aatcccgcag agcatccgga ttgtcgtgga cttgcccgcc
      541 ccgttcggac ccagaaatcc gaatatctcg cccctgttca cgcgcagcgt cacccggtca
      601 acggcaacga attgcccgaa tttccgcgtg agatcgcgcg tttctattat cggcgcggac
      661 ccttcagcca tcgtgacttc cgccttcagt cctgtgtccg ctcggcgccg accacgtacg
      721 caaggttggc cgccgccgtc ctctggtccg tccgtgcctg taccagatca agccgcgccc
      781 gttccagcag aatctgcgca tccagaacct ccgtgttcgt cgccgtcccg gcggagtacc
      841 gcgcacgcat gttccgatga tatgcctcgg cgctccgcac tccttcctcg gcaactgcca
      901 cccggcggtt cccctcgatt accgcaagac gcgcctgcac cacctgcaac gtgatggcgt
      961 cgcgcacctg gttcaatcgc tcgtccacct gccgctcttc ggcgcgcgcc cgcgccgcct
     1021 gctgcccgac cataccccag tcccaggccg tccacgtcac tcctataccg gcgttccatg
     1081 tggcgtgcca ggcatcccgg gtcggaaagt agcgctgatt cgggtttgat acttcgtagt
     1141 tcgtctgaaa catcaccgaa ggaagttttc ccccctccgc aaccgcggtc ccgcgctcca
     1201 tcgcctgctt ccggaacgcg agcgcgcgaa gctccgggcg gttcgcaatc gccgccgata
     1261 ccagcgcgtc ctgttctgga acggtcggca aaaccaccga cggcgagtcc accagggcaa
     1321 tttccgtggt taccggaacc gaaagcagat tgcacagcgt ggcgcgaacc agctcggacg
     1381 cgttctccgc ctgaatctgc cgcagtttcg cctccgccag ccgaacctga accgccagaa
     1441 tgtcgtctgc cgtcgccatc ccggctgcgt gcagccgtcg cacatcctca agatgagcct
     1501 cggcgagccg taccgactcc gaaatcacgc gctccgtcgc ccgcgccgaa tgcagccgcc
     1561 agtacacctg ctccacccgg ttcccgactt cggattgact tgccgaaaag tcctctccgg
     1621 cagctttcgc agcgtattca gccgccttct cgcggttctc cagacgaaaa cccgtgaaca
     1681 gcggcacctg cgccgtcgca gtcaggttgt acgaattcag aacggaaggc ccgagagaaa
     1741 cctgcgtcga gggcgtcgta atgctggttc ccggaccggt tgggatcgtc agggaaggcg
     1801 tcatgatcac cgggtccggc acatcgctga gtcgcgcata ccgcgcgctg agcgacacac
     1861 ccggcaaccg cgccgcgcgc gcctcgttct tctgcgccgt ggccgaaata accgattggg
     1921 cagaggctct gataccggcg ttctgggtca tcgcaagctc acgcgctcgc gctaccgtga
     1981 ggcggagcgc gttccccgcg gcctccgttt gagcaacacc cgaactccag agccccagcc
     2041 cggtgccgat agccgcgagg cttacgccgt accaccgtgt ccttctcatg ctgcggtgcc
     2101 tccttcggag ggattaaccg gcccttccga atgccgtatc tcggatatga atgcgtcctc
     2161 aagcgacggg ggaatctcac ggatggacgc cacgcggaat tgcatctctt ccagcgcctc
     2221 ctgaagccgt tgcgtacccg caaccacgtc ctccaccgga acatggatgc gcgaaccgaa
     2281 catctgcgct tcctcccatt ccggtgcctg tttcatggcg cggtacgccg cccgcgcgtc
     2341 ctcgcacaca acttccagca gccgcttccc gaatgtctcc acaatcgccg ccggggtgtc
     2401 ccttcggata atcctgccct cgtgcatcag cgccaccgtg tggcatcgct ccgcctcgtc
     2461 cagatacggc gtcgccagca caacggtgat cccctcgcgc agaaggtcgg agaggatgat
     2521 ccagaaatcc cggcgcgaaa ccggatccac ccctgtcgtc ggctcatcga ggaatatcag
     2581 gccgggcgaa tggatcagcg tgcacgccag cgccagtttc tgcttcattc cgccggaaag
     2641 gttccccgca agacgccggc ggaacggcgc cagccgcgtg aacgagagaa gctcttccct
     2701 gcgcttctgg aatcgccgta ccccgtgaat ctgcgcgaaa aactcaatgt tctcgtcaac
     2761 cgtcagatcg ccgtacagac tgaacacttg cgagagatac ccgatgctcc gcttcaccgc
     2821 gtcccgttcg cgaaccagat catgtcccag aaccgtcgcc gtcccggagg tcggtttgag
     2881 cacaccggtc agcatccgga tcgtcgttgt cttaccggct ccgtccgggc ccaccagcgc
     2941 aagcatctcg ccccggccca cggaaaaact cacgccgttc accgcgtgcg tggtgccgaa
     3001 aaacctcgtc aattctcggg tttcaagcgc tgctgcgggc atggttccgc cggcgcgcgc
     3061 aacatggtcc gtcattgcgg tgcaccggct ccgtcaccgg ctcgaaacgc cgcgtccgcg
     3121 ggcagtcccg gtttgaagaa cccgtccgga ttctgaagcg aaatcttcac ggcgaacacg
     3181 agcttcaccc ggtcttcctt cgtctgcacg ttcttcggag tgaactccgc cttcggggat
     3241 atccaggata ccctaccgct gcgcggatga tcaggctcgc cgtccagcgt tacctccgcc
     3301 gtgccgccca gccgcacctt tgccagctcg acttccgtca gatacacctt cagccagacc
     3361 gggtccagtt ccgatagcgt cacaatggtc ccgcccgagg gaaccatctc ccccggttcc
     3421 gcgatccgct gcgtcacgta acccttcctg ggcgacctca ccacgcagtc ggcgatccgt
     3481 tgagagacgg cactcaatgc cgcttccgcc tgctccacct gcgcccgcgc cgccgcgatc
     3541 tgctccgaac gcgttcccgc ccgaagtttt gccagcgtcg cccgtgccat cgcctgtgcc
     3601 gcttcggcgg accgcacgcg cgtccgcgcg tcatcaagct gtttctgggg tgccgtcccg
     3661 gtcgcggcaa gctggctcac ccgttccagg tcgagcctcg ccgcttccag cgcaaccgaa
     3721 gcctgttgct ccgccgcctc cgcctgcgcg atgtcctccc gttgcgggcc gttcaacgca
     3781 agctggagat tcgcccgcgc cacacgcagt tgcgcctcag cctgcgattt ctgccagtcc
     3841 aggtccgagt gatcaatcac cgccacaacc tgcccagaat ccagcagatc gccttcccgc
     3901 ggcagaacct cccgaaggat gcccggaacc ttcgtggcga tgttgatctc ggtgcactca
     3961 ataaccccgc tcgccgtgat gtccccgtca gatgcacccc gcgagcacgc gcccaggccg
     4021 atcgccagca ccggagctac ccaagccatt tttcccattt gtcttccctt ggaatcgtgg
     4081 acacccgtgt cgaatcctgt tccttcagca cgccgtgttg caggatctcc atgacgttcc
     4141 gcctccggtc ggcaaaatac tgaccggctt ttcggtcttc tatctggagc atggaactca
     4201 gaagtggctc tgcgaggaag gaaaacgcaa ccatcccgag aaccgagatc aacgtgtgtt
     4261 tcgggtcaat ctcgtggatg acgccctcgt ggattccccg ccggatcaga tcaagcgccg
     4321 gtttcgcgcc gacaaagctc gccctgtgct gcgcctgcgg aagcgcccgc atcacgatct
     4381 ccccgccggc cagcaggtcc atcatgatca gccgcggcac atggggtttt ttcgaaaggg
     4441 aatcgaggta cgtgtcgacc agcatcagga accgcttctc cgtgggcacg tcttcgccct
     4501 tcagaacgcc ctgcacgcgt tccatgatcc cctgaactgc agcggatacg acggtttcgt
     4561 acagcttttc cttgctggtg aagtagtaat gcagcatcgc tttgttgatt tgtgcccggt
     4621 cagcgatctc ctgcattcgt gctccggata gccctttgtc gacgagcact tctgttgcgg
     4681 catcgagtat ccgttggcgg gcatctgagt ccctgttcat ggggctttct cctgtcgcag
     4741 ctcaaccaat cagtcggttt aaccgaccgg ttagttagca tacgaaatca ctagccagcc
     4801 tgtcaagctc cagctcgacc gttccccgtt acgaccccca ccggctggcc cgtccggcga
     4861 tccatcccgc caccgcaatc tgcacggaga caagaaccgc acacacgccg ataaccgtca
     4921 aaaggctcag aatcccgctt ccagccagca tttccgcgcc aaaaagagaa agaacgacgc
     4981 gccatgtatg cggatccccc agagcaccga aaaagcccgc agcaaggcac tccacgccca
     5041 gtacgaagcc gatgaaacgc acctgtttcc gaaccccgca ctccacgcca tgccgctcgt
     5101 acgcggcgcg acgtccgaga tagaccgccg caagccaccc gaggagaaac gcgccaaaag
     5161 gaaaccccat gaaaaacgca aaggaaaaca ccgaccagat gacgtacaga accgcgagat
     5221 ggcccatcgg aatcgagaac actctgcctg catttctccg gtaccacacc catgccagaa
     5281 aaacccccgc cgcaaggccc gaacacgccg cccatgcgat cgttgagtcc gtgaggggaa
     5341 cccatctgag aattgacaga atggcggcgc tccaccacga cagaatgccc gctgcaaggg
     5401 caattgacgc gcctgtgaga gctgcgaaca acttcatctc catccgcacg gaaccacccc
     5461 tttgccgcca gagtccagac accgcttccg atgtgaataa ctgcgcagac gggttcagga
     5521 caaaatcgcc ctggctgcgg gcggggcggg acgttgaccg ctcgggaggt tttttatagc
     5581 gtacgtaggc atacgcggcg ttttttctgc gtctgttacc caccttttcc gcccgcgaac
     5641 gattgccgcg ggtgaatccc gtaaccgaga gataccggat gaacgttcct gtgcaggaga
     5701 gaatcgttgt tctcgatttt ggcgggcagt acgcgcatct tatcgccaac cgggtgcgtc
     5761 ggctccgcgt gttttcagaa attatgctcc cgtccgagcc gccggcgaat cttgaaggtg
     5821 ttcgcgggct gattctttcc ggcgggccct cgtcggttca cagcccggac cagcccccgt
     5881 ttgacgcccg ctggctccac accggattgc cggtgctcgg cctgtgctac ggccaccaac
     5941 tgcttgcgaa ggaactgggc ggggaagtgc ggcggggcga tcgccaggag tacgggattg
     6001 cggagcttca tatcaccgac cacgacgcta ttttcgaggg tcttgagccc gtggagcgcg
     6061 tctggatgag ccacggagat tcagtaaccc gcgttccgga tggatttgaa gttctgggtg
     6121 gaacggcgga ttgcccaatc gccgcgatgg gtgacgttca gaggcgcatg ttcggattcc
     6181 agtttcatcc agaagtgacg cactccccgc acgggcagaa aatgctggac aatttccttt
     6241 ctctgtgcgg atgcgcgcgc acctggacga tggagc
//
LOCUS       DAOTNG010000183         6266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10713_length_6266_cov_5.218000, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000183 DAOTNG010000000
VERSION     DAOTNG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_6266_cov_5.218000"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="P5179_09355"
     CDS             <1..328
                     /locus_tag="P5179_09355"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965238.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRS95464.1"
                     /translation="TCAIVDEPETAVRDADVIYTDVWASMGQEAETETRRTAFSRYQV
                     NGALLRSAPEHAVVSHCLPAHRGEEITSEVLDSARCIAFDEAENRLHVQKAVLLFLLG
                     GESVGD"
     gene            273..1976
                     /locus_tag="P5179_09360"
     CDS             273..1976
                     /locus_tag="P5179_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRS95465.1"
                     /translation="MSRKLFCFSCWVGNRLAISARVYLRIVPCLALCCAACARVGSPG
                     GGPEDTIPPRVVRMAPEDSATRVSLDMNPRIEFSERMNKASADQAIFITPYPVRYPRF
                     EWSRGDRVLTIRFEGGLEPQKTYVITIGTGVTDLHGVRLASAQAFAFSTGDSIHRGRV
                     QGVVYDGRFVPTSGLTIGLFPLQDNPSPDPAREYAAYVTQSGNAGRFSIGYLPDGTYR
                     LFAWRDANSDGLIDAEEPVGLASTDVVVKDGAHVRIPSVCMGLSDRTGPRLERIRALD
                     ATHVEMLFGEPVDSLRVEAVEPASGATPIECSVLKTPRASNRVVVYCSGLKPGAAYGA
                     HVRAWDRAGNPAEWAADTTSFVASGQAVTMRLRAESVEDNRAISPDSPAGFSVWTNDL
                     AVPATGFGRAFQDDGTIVEGAWHQRHANHFMFAANTLMGSRERNWVVPLADLRDDRGN
                     PCEDSVRVTLYPAREDSMGEVSGVVSDSIPGRNLRVLLRKTEEGPIFPAVVSDSLSWH
                     ANGLPAGDYEVFAWYDEDGNGSWSPGNIIPFAPAERCAVFGTVAIRPRWTTAGVVVHF
                     E"
     gene            2044..2772
                     /gene="rph"
                     /locus_tag="P5179_09365"
     CDS             2044..2772
                     /gene="rph"
                     /locus_tag="P5179_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRS95466.1"
                     /translation="MPNRPDGRQPDEMRPVKITRGVSPYAEGSVLIEIGGTHVMCTAT
                     VEESVPPFLRGQGNGWVTAEYGMLPRSSSIRIPRESATGRIKGRTHEIQRLIGRSLRA
                     VIDLACLGERQIILDCDVLQADGGTRTASITGSWIAMADAVRWMSEKNMIRKDPLLDQ
                     VAAVSVGISDGLPVLDLCYEEDSRADVDLNLVMTGTGCFVELQGTAESVPFTDDQLAQ
                     MITLGKKGIAELLAIQKRVCEDSL"
     gene            complement(2776..3501)
                     /locus_tag="P5179_09370"
     CDS             complement(2776..3501)
                     /locus_tag="P5179_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HRS95467.1"
                     /translation="MRTPSRGEETPLPIRRETPTHWIYLHGFGSDPLSPKAQFFNQQF
                     RAAGLPIAVPDLNVPSFEQLTITACLAEVERVISGFPSEAKVGIVGSSLGGLIALFAA
                     DRCKNVSYVMLLAPALGLFRVNFVGLGKPGVRAWERNGFLEVFHSPSNRNRRLSADFV
                     ADARQYDERNLRLSIPVTIVHGQRDEVVDPHLSITYARAHHNVKLHLVDDDHSLLASI
                     TQIWEWLRIDTRPRPNRDTASAH"
     gene            3784..5019
                     /locus_tag="P5179_09375"
     CDS             3784..5019
                     /locus_tag="P5179_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95468.1"
                     /translation="MGLTTSDKQYLRGLAQRVAEIAADPYYAERREMWYDFNDLNPKR
                     PMVLIFPEGAWSELLPDSTMKLEDPFWRSVEWWLRYTIYRGERLRDDNVIDPVFELSP
                     SRGAAGWGLETRMKPTTTERGARAYDPVMKTPEDFAKMRRPHLVVNHEVNKRNYSRLY
                     DAFGDILHIRWKRSPGINTSLVNFVCYLRGLEQVMVDMVDRPDWLHEVFSFLLENTRV
                     LLDELEASGQLELMDGADYAGSGGTCYTRQLPASDYAGKARLKDTWGFAESQEYALIS
                     PRMYYEFGIKYQAPLLERFGLNCYGCCESLTDKLDLVIQHIPKLRRISISPWTDVRVA
                     AEKLQNKYVFSWKPNPVCVSNPYDPQTIRASIRETLEITRGCVVEMILKDTHTCNNVP
                     GRMEEWVRIALEEAERAAG"
     gene            5089..6162
                     /locus_tag="P5179_09380"
     CDS             5089..6162
                     /locus_tag="P5179_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRS95469.1"
                     /translation="MFGTTMDAIAKRKQFRAKRCSSYDRSGRNADWVLVKARSTHVMA
                     DINGAGVISHLWFTIAGHGRDNDYLRKMVFRAYWDGETQPSIETPVGDFFCVGHGAVV
                     SFQNAAFNMSANEDGYGKNAAMNCWLPMPFRTNARLEITNDAEVDIGMYYYVDYQEHD
                     RLDDDLLYFHAQWRRNNPCDGWKGPGSVWGSHEHGVRHNGPDGVNLSGEKNYVILEAE
                     GQGHYVGCNISIHNLYKGWWGEGDDMIFVDGEVWPPSLHGTGSEDYLCHAWGMQKNAF
                     LYNGLSYSEVEKEFNDRGKVTVYRLHLQDPVPFTRSLKVTIEHGHANDRSDDYSSVAY
                     WYQTEPHKPYPEMPGVVARLPNP"
BASE COUNT         1319 a         1674 c         1995 g         1278 t
ORIGIN      
        1 cacgtgcgcg attgtagatg aacctgaaac cgccgtgcgc gacgccgacg tgatctacac
       61 agacgtgtgg gccagtatgg gacaggaggc ggagacggag acgaggcgca cagcgttctc
      121 ccggtatcag gtcaacggag ccctgttgag gagtgcgcct gaacacgcgg tggtatcgca
      181 ttgcctgccc gcccaccggg gtgaagagat cacgagcgag gtgctggatt ctgcccgctg
      241 tatcgcgttc gacgaggcgg agaaccgact ccatgtccag aaagctgttt tgcttttcct
      301 gttgggtggg gaatcggttg gcgattagtg ctcgggtata cctccgaatc gttccctgtc
      361 ttgcgttgtg ctgtgcggcg tgcgcccgcg tgggttctcc gggaggggga ccggaggata
      421 caatcccgcc acgtgttgtg aggatggcac cggaagacag cgccacgcgt gtctctttgg
      481 acatgaatcc ccggatcgaa ttctcggagc gaatgaacaa agcttctgcc gatcaggcca
      541 tcttcatcac accctatccc gttcggtatc cgcgtttcga atggtcacgg ggcgaccggg
      601 ttctcacgat tcgattcgag ggtggtctgg aaccacagaa aacgtatgtg ataaccatcg
      661 gtacaggagt cacagacctg cacggcgtca ggcttgcttc cgcgcaggcc ttcgcgtttt
      721 ccacgggcga cagcattcac cggggacgcg tgcagggagt ggtgtatgac ggtcgcttcg
      781 tgccgacttc agggcttacc atcggccttt ttccactgca agataacccc agcccggatc
      841 cggcgcgcga atacgctgcc tacgtgacgc agtcgggaaa cgccggtcgc ttctcaatcg
      901 gttatttgcc ggatggtacg taccgcctgt ttgcctggcg cgatgcgaat agtgacggtc
      961 tgatcgatgc agaggaaccg gtggggctcg cgtcgactga cgtggtggtc aaggatggcg
     1021 cccacgtaag gattccttcg gtctgcatgg ggctttcgga ccgcacaggg ccgcggctgg
     1081 aacgaattcg cgcgctcgac gccacacacg tggagatgct tttcggtgag ccggtggatt
     1141 ccttgcgtgt agaggcggtt gagcctgcgt ccggtgccac accgatcgag tgctcagtac
     1201 tgaagacgcc acgtgcgtca aacagagtcg tagtgtactg cagcgggttg aaaccggggg
     1261 cggcgtacgg tgctcacgtg cgtgcatggg accgagcggg gaacccggcg gaatgggctg
     1321 ccgatacaac gtctttcgtc gcgtccggcc aggctgtgac gatgaggctg cgcgcggaat
     1381 ccgtcgagga taaccgtgcc atttctcccg actctcccgc cggtttttcc gtgtggacaa
     1441 acgaccttgc cgtgccggcg actggattcg ggcgcgcttt tcaggacgat ggcacgattg
     1501 tcgaaggagc gtggcatcag agacacgcca accacttcat gttcgcagca aacacgctga
     1561 tgggctctcg agagaggaac tgggtggttc cgttggcaga tctgagagac gatcgcggaa
     1621 atccgtgcga ggactcagtc cgtgtgacgc tgtaccccgc tcgcgaggac tcaatgggcg
     1681 aggtttccgg cgtagtaagc gactctattc ccggcaggaa tcttcgcgtg ttgttacgaa
     1741 aaacggagga agggccaatt ttccccgccg tggtctccga ttctctgtca tggcacgcga
     1801 atgggttgcc tgcgggtgac tacgaggtgt ttgcgtggta cgacgaggac gggaacggga
     1861 gttggtcgcc ggggaacatc atcccgtttg caccggcgga gcgttgcgct gttttcggca
     1921 ctgttgccat ccgtccacgg tggaccacgg cgggagtcgt ggttcacttc gagtgacgcc
     1981 tgtctctcac cgttcgaagt cacgggacga tttcgtgtcc tgctcagtta ctggaggtat
     2041 gcaatgccga accgccccga cggaagacaa cccgatgaaa tgcgacccgt caaaatcacg
     2101 agaggcgtgt ctccctacgc tgagggatcg gtcctcatag aaatcggagg cacgcacgtg
     2161 atgtgcactg ccaccgtgga ggaatctgta ccaccattcc tgcgcggtca ggggaacggc
     2221 tgggtgaccg cagaatacgg catgcttccg cgttccagca gcattcgcat ccccagagaa
     2281 agcgcaaccg ggcgcataaa aggacgaacg cacgagatac agcgattgat aggacgttcc
     2341 cttcgtgcag tgatcgacct tgcctgcctc ggggaacggc agatcattct ggactgcgac
     2401 gtgcttcagg ctgacggcgg gacccggacc gcttcgataa cgggttcgtg gatcgcaatg
     2461 gcggatgctg tccgctggat gagcgaaaaa aacatgattc ggaaggaccc gctgctggat
     2521 caggtggctg ctgtctcggt cgggatatca gatgggttac cggtgctgga cctgtgttat
     2581 gaagaggact ctcgcgccga cgtggacctg aaccttgtga tgactggcac cgggtgcttc
     2641 gtggaactcc agggaaccgc ggaatcagtc ccgttcacag atgatcagct cgcgcagatg
     2701 atcacgctgg ggaaaaaagg tatcgcagaa ctcctcgcga ttcagaaacg tgtgtgcgaa
     2761 gactccctct gagaatcagt gcgccgaagc ggtgtcgcgg tttggccgtg gacgagtatc
     2821 tatccgcaac cattcccaga tctgtgtgat gcttgcaaga agactgtgat cgtcgtcaac
     2881 gaggtgcagc ttcacgttgt gatgcgcccg cgcgtatgta atgctgaggt gcggatccac
     2941 aacttcgtct cgttgcccgt ggacaattgt tacggggatg gaaagccgga gattgcgctc
     3001 gtcgtactgc cgagcatctg cgacaaagtc ggcggagagc cggcgattcc tgttggaggg
     3061 agagtggaag acttccagga agccgttccg ttcccacgcg cgcacgcccg gtttcccaag
     3121 cccgacaaaa ttgacgcgaa aaagccccag cgcgggagcg agaagcataa cgtaggagac
     3181 gtttttgcac cggtctgctg caaacagagc gatgagccct cccaggctgg aaccgacgat
     3241 tccgactttt gcttcgctcg ggaatccgga aatgacacgt tcaacctcgg cgaggcatgc
     3301 cgtgatggtg agctgttcaa acgaaggaac attgaggtcg ggcaccgcaa tgggcaaacc
     3361 ggcagcgcgg aactgctgat tgaaaaactg cgcttttgga gacagcgggt ctgaaccaaa
     3421 cccgtgaaga tagatccagt gagttggcgt ttcacgacgt atgggaagtg gcgtctcctc
     3481 gcccctggac ggcgttctca cgcgagaaac cctctgtgca cgcggcaaag ggggcacttc
     3541 gccggcttct gggcgcacac gggataaccc gtccagctgc ctggtacgga acccgcggcg
     3601 gccgggcgga atctgtcggg gaccctgcat gtgttcagtt cctccatctt gcttctgctc
     3661 tatcgaaaga tacgttgagc ctgccggacg agcgaaacgg aatgtgatgc cgggcaacgc
     3721 ggggcgtggg tcgcctggcc cggggcgcgt acattaatac cgcgtttact caggaggctt
     3781 ctaatgggtt tgacaacctc agacaagcag tacctacgcg gactggcgca acgtgtggcg
     3841 gaaatcgccg cagacccgta ctacgccgag cgtcgggaaa tgtggtacga tttcaacgat
     3901 ctgaacccga agcgtccgat ggtgctgatt ttccctgagg gggcgtggtc ggagctgttg
     3961 cccgactcca cgatgaagtt ggaggatccc ttctggcgct ctgttgaatg gtggttacgg
     4021 tacaccattt accgcgggga gcggctgcgc gacgacaacg tgatagaccc cgtgtttgag
     4081 ctttcccctt cccggggcgc cgccggatgg ggcctggaaa ctcgaatgaa gcccacaacc
     4141 accgagcgcg gagcgcgagc ctatgacccg gtgatgaaga ctccggagga ctttgccaag
     4201 atgcggcgcc cgcatcttgt ggtgaatcac gaggtgaaca agcgcaacta cagtcgattg
     4261 tacgatgcgt ttggagatat cttgcacatc cgttggaaac ggtcgccggg gattaacacg
     4321 agcctcgtca acttcgtgtg ctacctgcgc ggactggagc aggtgatggt cgacatggtg
     4381 gatcgtcccg actggctgca cgaggtattt tcatttctcc ttgagaatac ccgcgttctt
     4441 ctggacgaac tcgaggcgtc cggccagttg gaacttatgg acggtgccga ttacgcgggt
     4501 tccggaggga catgctacac gaggcagctc ccagcgagcg attacgcagg aaaagcgcgc
     4561 ctcaaggaca cgtgggggtt cgccgaatcg caggagtatg cgctgatttc tccccgcatg
     4621 tattacgagt tcggaatcaa gtaccaggcg cccctccttg aacgctttgg ccttaattgc
     4681 tacggatgct gcgaatcctt gaccgacaaa ctggatcttg tcattcagca cattccgaaa
     4741 ctccgaagaa tctccatctc gccgtggacg gacgtgaggg ttgcagccga aaaactccag
     4801 aacaagtatg tgttttcctg gaaaccgaat ccggtctgtg tatccaatcc ctacgatccg
     4861 caaactattc gggcgagcat tcgcgaaacg ctggaaatca cgcgcggttg tgtggttgag
     4921 atgattctga aagacactca cacatgcaat aacgtgccgg gccggatgga ggaatgggtc
     4981 cgcattgccc tggaagaagc agagcgggcg gccgggtgag cgaacgcagg ggcggtgatc
     5041 gccctgcgtt gtcgaggcgc gaaatgcgcc gggaaacggg aggcatttgt gttcggaacg
     5101 acgatggatg cgattgcgaa aagaaagcag tttcgagcga agcgctgctc gagctatgac
     5161 cgctcgggcc ggaatgctga ctgggtgctg gtcaaggctc gaagcacgca cgtcatggcg
     5221 gatatcaatg gagccggggt aatcagtcat ctctggttca caattgcggg acacgggcgg
     5281 gataacgatt atctgagaaa aatggtgttc cgtgcgtact gggacggcga aacccaaccc
     5341 tcgatcgaaa ccccggttgg cgatttcttc tgtgtcgggc acggagcagt agtttcgttt
     5401 cagaacgcgg cattcaacat gtctgcaaat gaggacggtt acggcaaaaa cgcggcgatg
     5461 aactgctggc ttcccatgcc gttccggacg aatgcgcgac tcgaaatcac aaatgacgcg
     5521 gaagttgaca tcggcatgta ctactacgtt gactatcaag agcatgatcg gcttgacgac
     5581 gatctgctct attttcatgc gcagtggcgg cgcaacaatc cgtgtgacgg gtggaaaggg
     5641 ccgggatcag tctggggcag ccatgagcac ggcgtgagac acaacgggcc ggacggagtc
     5701 aacctttccg gagagaagaa ctacgtcatt ctcgaagcag aggggcaggg gcattatgtg
     5761 ggctgcaaca tctcgatcca caatctctac aagggctggt ggggcgaggg tgatgacatg
     5821 atttttgttg atggggaagt gtggccaccc agtctgcacg gtaccggaag tgaggattac
     5881 ctgtgtcacg cgtggggcat gcagaagaac gcgttcctgt acaacggcct ttcgtacagc
     5941 gaagtggaga aggagttcaa cgatcggggc aaagttaccg tgtaccgtct gcaccttcag
     6001 gaccctgttc ctttcaccag gtcgctgaag gtgacgatcg aacacgggca cgcgaatgac
     6061 cggtcggacg attactcttc cgttgcgtac tggtatcaga ccgaaccgca caagccttac
     6121 cccgagatgc caggtgttgt cgccaggctg ccaaatccct gaaaagacgg ggatgccctc
     6181 ttacgacacc ggaaactcgg cgctcgagag agcgcgagtt gtggctgagt ggttgcggaa
     6241 aaagggcgtc gtcgaacctg cgggtt
//
LOCUS       DAOTNG010000184         6221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10802_length_6221_cov_5.154882, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000184 DAOTNG010000000
VERSION     DAOTNG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10802_length_6221_cov_5.154882"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1537
                     /gene="rmuC"
                     /locus_tag="P5179_09385"
     CDS             8..1537
                     /gene="rmuC"
                     /locus_tag="P5179_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRS95470.1"
                     /translation="MLALIVGLLIGVAAALLVARNRIERARTQAATEAATARAVLEER
                     LRSTDELAASLRGQLAVLQDDLRHRTEQLQQEAERRSAAEAQAARIPELESSLEALRA
                     SNADLEKIRSELETRIAEERKAADEKMAMLQQARTELSDAFKALSSEALNSNNKAFLE
                     LAKTHLEKFQEGAKTDLEARQKAVEELVKPVKESLVSMDTKLQELETKRVSAYAELLT
                     QVNAMKDQQSQLRNETSRLVQALRTPNVRGRWGEIQLRRVVEMAGMLNYCDFDEQVTA
                     KEGEIRPDMIIRLPGGRNIVVDAKTPILAYLDAIETDNEEKRKEHLARQAQHVREHIK
                     KLSAKSYWDQFDNTPEFVVLFLPAESLFAVALEQDRTLIEEGVKQRVIPASPITLIAL
                     LRAVSYGWRQEDIAKSAEEIAAVGKELYDRIANMADHLDRLGDRLKGAVDSYNKAIGS
                     FETRVLVSARRFKELKAAAGNEIKEIPQLDIVPRTVDIPDFPTPRLSPPAPDNELPAG
                     A"
     gene            complement(1732..2868)
                     /locus_tag="P5179_09390"
     CDS             complement(1732..2868)
                     /locus_tag="P5179_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95471.1"
                     /translation="MAIGTCFPLGMYELPRNEEEWAHWKSAGINLLPCASREDLDSVH
                     RHGMKGWVSLPMVLREDDDGAALRAAVSALKDHPALYVWEAPDEIIWNTNRRVTGVLA
                     SFWWDQPERMRKELAQEYDALVRGLERGSAIVRSLDPGRPIWLNEAARSDMEIVSRCL
                     PFLDIISTDFYPVLPQTPDPRRARIRTHDMIALLCDRFRATAPDHEFWFVQQAFSWHR
                     LERKRHPDVPIAYPTRCDYRFMAWQAIAHGARGLLWYGSAHEDRPAPFLDDLMAVVRE
                     LSELSPFLLSEHAPVLEVRVDTKQYPPTRGVSCFMREFGGRTFLALVNEDPYENDVLV
                     TGVPGEEITGWTAFGDSERPHVPIDNGFVVPLSGREVRLMMSAA"
     gene            complement(2978..4225)
                     /locus_tag="P5179_09395"
     CDS             complement(2978..4225)
                     /locus_tag="P5179_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRS95472.1"
                     /translation="MPDPVILAIFGLVAGCYLAWTIGANDVANAMGTAVGSRVLTLGA
                     AVAVASVFEFSGAFFAGGQVTNTIRYEVINVPLVEKVGPFMGVGMLAALLASAVWLHI
                     ATWKGWPVSTTHSIIGAVAGFGFAASGGDAVRWMTLAGIGTSWVLSPFLGGLFAWVLF
                     RVIRRAVFAHDHPARRARMVTPLLIGVTGAVLTLSMVYKGLANLHLDLPIGHALALAG
                     CVGVLSAVGARVVLGRRQDHGADRDTQSRNVERQFRPLLVITACYVSFAHGSNDVANA
                     VGPMAAVVVSSKAGFLSYFHDPATILMALGGAGIVVGLATYGYKVIETIGKKITEVTP
                     SRGFVIQFSTASITLLGSKLGLPVSTTHIIIGAVVGVGFARGLSAVNTGMVRQIVWSW
                     LITFPAAGLLCVVFFHVLRPLFL"
     gene            complement(4265..4939)
                     /locus_tag="P5179_09400"
     CDS             complement(4265..4939)
                     /locus_tag="P5179_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HRS95473.1"
                     /translation="MARSILALFSHSPWGAIQEHMRAVDKCVQLLDPLMDSVLAGNYP
                     RTEQLAREISALENAADEIKNELRDHLPRSIFLPVARQDLLDILSTQDGISDAAEDVG
                     MIVTMRKMEVQPALVDLLDPFVQKTLESVASYSQVIGELDRLVEASFVGKEAERVTEL
                     IRKVGRLEHEADNAQEALSRRLFEIEDSMKPGALFMWIEIIRRLGSVANRAEKAANRL
                     RMFLAH"
     gene            complement(5093..>6221)
                     /locus_tag="P5179_09405"
     CDS             complement(5093..>6221)
                     /locus_tag="P5179_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS95474.1"
                     /translation="AVWESGDRLQHRVDLYPGSGFFLYGTVRYAGGIAVSGISFTVTG
                     ENALAQATSGEDGIFVAGPFAVGTHRVGVTITGETPFRPAVTAFDAAQVLRAVVTGAP
                     LGAPMAVADVTGDGSLTTHDAAEILQFVVGRRKVFSGGRLAVAVPESLTVTGTLGETV
                     PRDLSIQAVGDVTGNWRQTAKPALFDAELVKIGSSDGAAWRLYTESPVFSVIAAFEGG
                     SVVPEFPSDWLVETVRSADQVLIAAAGARPLSPGAVVVRSTSGEVRFVKGSVNEEASL
                     RSVGNPTPAQVTLSVSPNPFNPRCTIRFDCPEPVQVRLSVYTVTGQFVRELAAGHRDA
                     GSFSEVWDGRDHSGAPVASGVYLVRLDAGSSRKLVRVVLSR"
BASE COUNT         1378 a         1996 c         1771 g         1076 t
ORIGIN      
        1 gcccgagttg ctcgccctca ttgtaggcct gctgatcggc gtcgccgcgg ctctgctcgt
       61 cgcgcggaac cgtatcgaac gcgcccggac acaggcggca accgaagcgg cgacggcgcg
      121 cgccgtgctg gaagaacgat tgcgctcaac cgacgagctg gctgcgagtc ttcgtggcca
      181 gctcgccgtg cttcaggatg acctgcgcca ccgcacggag caactccagc aggaggccga
      241 acgtcgctct gcggcagagg ctcaggcagc ccgtatcccc gaactcgaat catcccttga
      301 ggcactccgg gcctcgaatg cggacctcga aaaaatccgt tccgaactgg aaacccgtat
      361 cgccgaggaa cggaaagccg ccgacgagaa aatggccatg cttcagcagg cacggaccga
      421 actttccgat gcattcaaag ccctttcctc ggaagcgctg aactcgaaca acaaggcctt
      481 cctcgaactc gcgaaaaccc atctggagaa attccaggag ggtgcaaaaa cggatctcga
      541 agcgcgccag aaggccgttg aagaactggt caaaccggta aaggaatccc tcgtctccat
      601 ggatacgaag cttcaggaac tggaaacgaa gcgtgtatcc gcgtatgccg agctactgac
      661 gcaggtcaac gcgatgaagg accagcagtc ccagctcaga aacgagacct cccggctcgt
      721 acaagccctt cgaacgccca atgtccgcgg cagatggggc gaaatccagt tacgtcgcgt
      781 ggtcgagatg gccggaatgt tgaattactg cgatttcgac gagcaggtga ctgcaaaaga
      841 aggcgaaatc cgccccgaca tgataatccg gctccccggc gggcgaaaca tcgtcgtcga
      901 tgcaaaaacc cccatccttg cctaccttga tgctatcgag acagacaacg aagagaaacg
      961 caaggagcac ctcgcgcgcc aggcgcagca cgtccgtgaa cacatcaaaa aactcagcgc
     1021 aaaaagctac tgggaccagt tcgacaacac ccccgagttt gtcgtcctgt tccttcccgc
     1081 cgaatccctt tttgctgtcg ctctcgaaca ggaccgcact ctcatcgaag agggcgtgaa
     1141 acaaagagtc atcccggcct cacccatcac cctgatcgcg ctcctccgcg cggtttcgta
     1201 cggatggcgg caggaggata tcgccaaaag tgcggaagaa atcgccgcgg tgggcaaaga
     1261 actttacgat cggatcgcca acatggcgga ccacctcgac cgattgggtg accgcctcaa
     1321 aggcgccgtg gacagctaca acaaagccat cggctcgttc gaaacccggg ttctggtttc
     1381 cgcccgccgc ttcaaggagc tgaaagcggc cgccggaaac gaaatcaaag aaatccccca
     1441 gctcgatatc gtcccgcgca cggtggatat tccggatttc cccacgccga ggttatcgcc
     1501 gcccgccccc gacaacgaat tacccgccgg cgcctgagca ccgcaacaca cttgcgcgga
     1561 ctcttctcgc ttccctgtca tttccgactg tggcaaggaa acgtgttcgt gtcaatactt
     1621 ttgccttcga tttttcaatt ctggtacagt tttttttgaa acctcgattc ggcggggctt
     1681 gcgaggaaac acgaagctcc ctccttcggg gttggacttg acgcggttcc ctcaggcagc
     1741 gctcatcatc aggcggacct cacggcccga caaagggacc acaaagccgt tgtctatcgg
     1801 cacgtgcggg cgttccgaat ccccgaacgc cgtccaaccg gtaatctcct cgcccggcac
     1861 accggtcacc agtacgtcgt tctcgtaagg gtcttcgttc accagcgcga gaaatgtccg
     1921 cccgccgaat tcccgcatga agcacgaaac acctcgcgtc ggggggtact gcttcgtgtc
     1981 cacccgaacc tcaagaaccg gtgcgtgttc tgacaggaga aaaggcgaga gttcggacaa
     2041 ttcgcggact accgccatga ggtcatccag gaacggcgcg ggccggtcct catgtgcaga
     2101 gccgtaccag agaagacccc gcgccccgtg agcgattgcc tgccatgcca tgaaccggta
     2161 atcacacctg gtagggtacg cgatcgggac gtcgggatga cgtttccgct caaggcggtg
     2221 ccacgaaaac gcctgctgaa caaaccagaa ctcgtggtcc ggggctgtcg cccggaagcg
     2281 gtcgcaaaga agggcgatca tatcgtgtgt gcggatcctc gcgcgccggg ggtctggagt
     2341 ctggggaaga acagggtaga agtcagtgct gatgatatcc aggaacggaa gacaccggga
     2401 aacgatctcc atgtcggatc gagcggcttc gttcaaccag atgggacgcc ccgggtccag
     2461 agagcggacg atcgcggagc cgcgttccag accgcgaacg agcgcatcgt attcctgcgc
     2521 cagttctttt cgcatccgtt ccggctgatc ccaccagaag ctcgccaaca caccggtaac
     2581 cctgcggttg gtattccaga tgatttcgtc cggagcctcc catacgtaca gcgccggatg
     2641 gtctttcagg gcgctgacag cggctcgcag cgccgcgccg tcgtcgtcct cacgcagcac
     2701 catgggcaat gaaacccacc ctttcatgcc gtggcgatgc actgagtcca ggtcctcgcg
     2761 gctcgcgcaa ggcagcagat tgattccggc ggatttccag tgagcccatt cctcctcgtt
     2821 gcggggtagt tcatacattc cgagcgggaa gcacgttcca atcgccatca tacgttctcc
     2881 cagaggcaac gcttggtcgc aggtcaaacc ctgccagatg gcccttcgac ccgcgtcagg
     2941 aaaccggtag ggccggggaa atcgggcacg gcagctctca cagaaaaagc ggtcgaagca
     3001 cgtggaagaa gacgacgcac aggaggccgg cggcgggaaa cgtaatcagc cacgaccaca
     3061 cgatttgtcg caccattccc gtgttgaccg cggacagccc tcgggcaaac cccacgccca
     3121 ctaccgcccc gatgatgatg tgggttgtcg acacaggcag ccccagtttc gacccgagca
     3181 gcgttatgga tgcagtgctg aactgaatga cgaaaccacg gctcggagtc acttccgtaa
     3241 tcttcttccc gatggtttct atcaccttgt agccgtacgt cgcaagaccg actacgatgc
     3301 ctgctccgcc gagggccatc agaatcgtcg cagggtcatg gaaatagctc aaaaatccgg
     3361 ctttgctcga caccacaacg gcggccatcg gcccgaccgc gttggctaca tcattcgaac
     3421 cgtgagcgaa cgacacgtag cacgccgtaa tcacgagcag cggacggaac tggcgttcca
     3481 cgttgcggga ttgggtatcc ctgtccgctc cgtggtcttg tcgccgtccc agaacaaccc
     3541 gcgcgcccac ggcggaaaga acacccacgc atcccgccag cgcaagcgcg tggccgatgg
     3601 ggagatcaag atggaggttc gccagcccct tgtagaccat ggagagggtc aataccgcgc
     3661 cggtcacacc gatgagcaac ggtgtgacca ttctcgcgcg tcttgcagga tggtcgtgcg
     3721 caaacaccgc cctccgtatc acccggaaaa gcacccaggc aaaaaggccc ccgaggaacg
     3781 gtgacagtac ccagctcgtc ccgattccgg ccagtgtcat ccagcgaact gcatccccgc
     3841 cgctcgccgc gaatccgaac cccgccaccg cgccgatgat ggagtgtgtc gtggacacag
     3901 gccacccctt ccacgtcgca atgtggagcc agactgccga cgccagaagt gcggcgagca
     3961 tgccgactcc catgaaaggg ccaactttct ccacaagagg gacgtttatc acctcgtacc
     4021 ggatcgtgtt ggtaacctgc ccgccggcga aaaaggcacc ggaaaactca aaaaccgatg
     4081 caacagctac cgcggcaccc agagtgagaa cacgagatcc gacggcggtt cccatcgcgt
     4141 tggcaacgtc gttcgcgcca atagtccacg ccagatagca cccggcaacc aggccaaaaa
     4201 tggcgaggat gacgggatcc ggcacgacgg gctcctggat tcgggggaac gaaagcggtt
     4261 gatgtcagtg cgcgagaaac attcgaagcc tgttggcggc tttttccgcc ctgttcgcca
     4321 cacttccaag acgacggatg atttcgatcc acatgaacaa tgcgccaggt ttcatcgaat
     4381 cctcgatctc gaacaacctg cgcgaaagcg cttcctgcgc gttgtctgct tcgtgttcca
     4441 gtctgcctac cttccgaatc agttccgtaa cgcgctcggc ttccttgccg acgaacgaag
     4501 cttcgacaag acgatccagt tcgccgatta cctgcgagta ggaggccacc gattccagcg
     4561 ttttctgaac aaagggatcc agcagatcca cgagcgcagg ctggacttcc atcttccgca
     4621 tcgtgacgat cattccgaca tcctccgccg cgtcggaaat gccgtcctgc gtggaaagta
     4681 tgtcgagcag gtcctgtctt gccaccggaa ggaagatact tcgaggcaga tgatcccgca
     4741 gttcgttttt gatctcatcg gcggcgtttt cgagggcgga aatctcacgt gcgagctgtt
     4801 ccgtgcgagg gtaattgccg gccagaacgg aatccatgag cggatccagc agttgcacgc
     4861 acttgtccac tgcgcgcatg tgctcctgaa tggcgcccca cggagaatgc gaaaacaggg
     4921 caagaataga gcgagccatg gcacaccccc tcggaaggta aaggcgagtc gcaacgtgtt
     4981 cacggaaagt atacggaaag ccgacctgcg gagaaatgcg ttggcaggcg gcgctcggtc
     5041 ccgtcggttt ttctcgctcc cgtggggaac ggacccgcca gcgggattct gctcaacggg
     5101 agagtaccac ccggacgagt ttccggctcg aacctgcatc caaccggacg aggtacactc
     5161 cgcttgccac gggtgcgcca gaatgatcgc gcccgtccca tacttccgaa aagcttcccg
     5221 cgtcgcggtg ccctgcagcg agttcgcgca cgaactggcc cgtcaccgtg tagacagaaa
     5281 ggcgcacctg caccggctca gggcagtcga accggatagt acaccggggg ttgaacgggt
     5341 ttggcgaaac ggacagggtg acctgtgccg gcgtcggatt accaacggaa cggagagacg
     5401 cttcctcgtt caccgaaccc ttcacaaagc gcacttctcc gctcgttgaa cgcacgacaa
     5461 cggctcccgg tgagagcggg cgcgctccgg ctgcggcgat cagaacctga tccgccgacc
     5521 ggacggtttc aaccagccag tcgcttggaa actcgggtac gacagaacct ccttcaaaag
     5581 cagcgattac ggagaaaacg ggactttcgg tgtacaggcg ccacgccgcg ccgtccgacg
     5641 aaccgatttt caccagttcc gcgtcaaaca gggcgggctt tgcggtctgc cgccagttcc
     5701 ccgtcacgtc gcccactgcc tgaatggaaa ggtcccgcgg cactgtttcg ccgagcgtgc
     5761 ccgtgacagt gagcgactcc ggaacggcaa cggcaagccg ccccccggaa aaaaccttgc
     5821 gtcgccccac cacgaactgc agaatctccg cggcatcatg ggtcgtcagc gagccgtcgc
     5881 ctgtgacgtc cgccaccgcc atcggggcgc cgagcggcgc ccccgtgaca actgcccgaa
     5941 gcacctgagc cgcgtcaaag gcagtcactg ccggacggaa aggcgtctcc cccgtgatcg
     6001 tgacaccgac ccggtgggtt cccacggcaa aaggccctgc aacgaaaatg ccgtcctccc
     6061 cgctcgtggc ctgcgcaagg gcgttttctc cggtgaccgt gaatgatatg ccggataccg
     6121 caattcctcc ggcatagcgg acggttccat acaggaagaa ccccgaaccg ggatacagat
     6181 ccacccgatg ctgcagcctg tcccccgact cccagactgc g
//
LOCUS       DAOTNG010000185         6214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10815_length_6214_cov_5.568599, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000185 DAOTNG010000000
VERSION     DAOTNG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10815_length_6214_cov_5.568599"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1339)
                     /locus_tag="P5179_09410"
     CDS             complement(227..1339)
                     /locus_tag="P5179_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95475.1"
                     /translation="MNEAIIPSIDPIALPAPVWLMQFLSGLTFIFHVIPMNVVLGGGV
                     VALFLAVWGKRGSRKNHVRLARRMTSVLPAAIAMTITLGVPPLLFLQAMYGPLFYTSS
                     VLMAWPWLSVIAILLVGYYGHYYFSLSNDFANPRALVVGAVAVVAFLVIAFLYVSNMT
                     LMVMPERFLGIQRSWTSGFLPFTGDSIFAPRFLHFITGAVSVGGLLAVFVGLLERSDA
                     EFKAVAVRAGGFTFVGATIVQIATGIWFLLALRPRVMMLFMGQDHAATALFGVAVLLA
                     LVAVALILVAVIGKKRPWMTIAGTACIGLTIIIMTVMRSIVREAYLGTRFVVAEQPVN
                     PAWDVVVLFLVLLVAGAALVGWMIHAVVTRRGLAAS"
     gene            complement(1336..2913)
                     /locus_tag="P5179_09415"
     CDS             complement(1336..2913)
                     /locus_tag="P5179_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRS95476.1"
                     /translation="MNYPFWDVPLLGGGLVIALIAIPHVFVSHFAIGGGIFLAWTERI
                     GIRKGKPEYIAFAKRNTLFFVLMTLVFGAVTGVGIWFSIGLVHPDATSALIHNFVFGW
                     AMEWVLFLVEIAAALLYYYGWDRVDQKTHQFIGWAYAGAAWGSLVVINGILAFMLTPG
                     RWVETGGFWTGFFNPSYFPSVLIRTCVCLALAGLYALLMASREPAGETHDKLVRYAST
                     WLLPAFIGLPIFAAWFVFTLPSLAQDMFMGRAVVLTIFAALSLGISAIIFAFAYLIAY
                     RNPQSVSTPVALLFLVLGLAVTGVTEFGREAARKPYIIYDYMYSNAVLKRDVARLQQE
                     GVLRNAKWALVSKATPQNALIAGREIFTLACAHCHTVDGYNGVRLLVKGWPEPFIEHQ
                     LTYLDQLKGFMPPFVGNPEERRALAKWLASLNPSALPARTAAAATDSLSADPLARGEA
                     AYSAHCANCHTIDGRNAIRQHVKGWTQSVAYERLGRLESLRGYMPPYEGPEPERQALA
                     AWLASIGNDAPTAGGAR"
     gene            complement(3003..4265)
                     /locus_tag="P5179_09420"
     CDS             complement(3003..4265)
                     /locus_tag="P5179_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS95477.1"
                     /translation="MSDRLAIDGGTPVRTAPLPPSWLGAYEMGVEEEEAVLKVLREKR
                     VFRYIAKEESETARLEKAVAEFTGRDYALAVNSGTAALICALVGLDIGPGDEVIVPGY
                     TYIATAAAVVTVGAVPVIAEVDNSLTLDPEDLERKITGLTKAVIPVHMRGTPCRMDEI
                     CAIAKKHGIKVIEDTAQANGGKYKGKTLGGIGDVGCYSYQMSKTITAGEGGMVVTGDR
                     LVFERILMAHDSAFGFWKPEETAIRPIPGEGFRISEVSGAIALVQMGRLRGIVERLHN
                     AKYRIVNQIRGLAGITLQDVPDESGDASVCLMFFAPDAEIANTYAKALNAEGIPCGTM
                     FSKEIPDRHIYPNWTYVLEKRGRSSNWSPWHPSVYKGSVEYSKDMCPETLDYLGRTIH
                     IGLSPRLTAADSDDIARAIRKVVTALAR"
     gene            4417..4803
                     /locus_tag="P5179_09425"
     CDS             4417..4803
                     /locus_tag="P5179_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95478.1"
                     /translation="MAEITQGTDPGICIVRFSQGARICGQLADLLNPVDPATFHQYRL
                     PARTPVELHFHDFDEYWWFVEGHPTVTLRGKDGRMVEEILQPPDMVACVRGFEHTLYA
                     DHDLVYYQFSSVRTGSEREGHLSRAK"
     gene            complement(4806..5543)
                     /locus_tag="P5179_09430"
     CDS             complement(4806..5543)
                     /locus_tag="P5179_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS95479.1"
                     /translation="MKALVLAGGYATRLHPVTLEASKPLLKVNGRPIVDYVLDGIETL
                     GDVDTTYVVTNAKFFQDYVEWAKTQQRPFDVVPLSDGTTSNADRLGAIGDIQFAIETQ
                     SVDDDLLIVGGDNLFDFSLRPLRDLQRAAGRSVLGCYDVGSLDLVSLYSEVHIEKGLV
                     SSFVEKPETPTSPLIGILCYALRRQDVPLVRRYLEEGRGPDKAGHLIQWMRTVTEVAA
                     YVFEGRWIDIGTRAELDKAEKQWSGPA"
     gene            complement(5578..5967)
                     /locus_tag="P5179_09435"
     CDS             complement(5578..5967)
                     /locus_tag="P5179_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRS95480.1"
                     /translation="MAHEVVVAKTEEWQDGSLGIIEVDGHRVALCRVGDAFFAFDDRC
                     PHAGSTLGLGTLHANAVECPRHQYLWRVTDGAPLTTAGPLTLFQVRVEGDSVLLTVPD
                     RWPEPAHWARHPKREELRRRHGFEEGE"
BASE COUNT         1368 a         1970 c         1691 g         1185 t
ORIGIN      
        1 acgttcgcgt atgatgatgc gtccgccccg cagagtcaga ccctgcgtga taccctggcg
       61 ttcctgcgga cgttggcgcc acgtttcacg atcaaagcga ccaccctcac tgaggcacgt
      121 gaagcgttca gggccatgtg cccggtttga tcgagcaatc gccacaaaac gagaaggcga
      181 acacgacctc cgtgttcgcc ttccttttcc gcggaacagg agttcgtcag gacgccgcga
      241 gacctcttcg cgtaaccacc gcgtgaatca tccagcccac aagcgcagct ccggcaacca
      301 gcagcacaag aaaaagaacc accacatccc atgccgggtt cacgggttgt tcggccacga
      361 cgaagcgcgt gccaaggtac gcctcgcgaa cgatggagcg catcaccgtc atgataatga
      421 tcgtcaggcc gatgcacgct gttccagcga tcgtcatcca cggtcgtttt ttgccaataa
      481 cggcaaccag aatcagggca accgccacga gcgcaagcag gacggcaaca ccgaagaggg
      541 ctgtggccgc gtggtcctgt cccatgaaca gcatcatcac gcgcgggcgg agcgcgagca
      601 ggaaccagat gcctgtcgcg atctgaacaa tcgtggcgcc gacaaacgtg aacccgcccg
      661 cccgtaccgc gaccgctttg aattcagcat ccgaccgttc cagcaatccg acaaacaccg
      721 caagaagacc accgaccgaa accgcgccgg taatgaagtg gagaaaacgc ggcgcgaaaa
      781 tcgaatcgcc cgtgaacggc agaaaaccgc tcgtccacga acgttggatc cccaggaaac
      841 gctcgggcat aaccatcaat gtcatattgc tcacatacag aaacgcaatc accagaaacg
      901 ccacgacggc gaccgcgccg acgaccagcg cccgcggatt tgcgaaatcg ttcgaaagcg
      961 agaagtagta atgcccgtag tacccgacaa gcagaatcgc aatcacactg agccagggcc
     1021 acgccatgag aaccgacgat gtgtaaaaca gcgggccgta cattgcctga aggaatagca
     1081 gaggcggcac gcccagagtt atcgtcattg cgatggccgc agggaggacc gacgtcattc
     1141 gtctggcgag ccgaacgtgg ttctttcgag acccgcgctt tccccaaacc gccaggaaca
     1201 gcgccaccac gccgcctccc agcacgacgt tcatggggat gacgtgaaaa atgaaagtca
     1261 gcccgctgag gaactgcatc aaccacaccg gtgccggcag agcaatggga tcgattgaag
     1321 ggatgatcgc ttcattcatc gcgcacctcc cgccgtggga gcgtcgtttc cgattgaggc
     1381 aagccacgct gcaagggcct gccgttctgg ttcgggtcct tcgtaaggtg gcatgtaacc
     1441 gcgcagactt tccagcctgc ccagccgctc gtatgcaacc gattgggtcc accctttcac
     1501 atgctggcgg atcgcgttgc gcccgtcaat cgtatgacaa ttggcgcagt gagccgagta
     1561 cgccgcttcg ccgcgggcca gaggatccgc actcaggctg tccgttgccg cggcggcggt
     1621 tcgcgccggc agagcgcttg ggttcagcga agcgagccac ttcgccagcg cccgccgttc
     1681 ctcaggattc cccacaaacg gaggcatgaa ccctttgagc tggtccagat acgtcaactg
     1741 gtgctcgatg aagggttccg gccagccttt cacgagcagc cgcacgccgt tgtacccgtc
     1801 cacggtgtgg caatgggcgc acgcgagcgt gaatatctcc cggccagcaa tcaacgcgtt
     1861 ctgaggagtc gccttgctca ccagcgccca tttcgcgttc cgcagcacgc cctcctgctg
     1921 aagcctggca acgtcccgtt tcaacaccgc gtttgaatac atgtagtcgt agatgatgta
     1981 cggcttgcgc gctgcttccc gcccaaactc ggtcacaccg gtaaccgcaa ggcccagcac
     2041 gagaaacaga agcgcaaccg gcgtggaaac gctttgcgga tttcggtagg caatcaggta
     2101 cgcgaacgcg aaaatgatcg ccgaaatgcc caggctcaac gccgcgaaaa tcgtgagcac
     2161 cacggcacgc cccatgaaca tgtcctgcgc gagcgaaggc aacgtaaaaa caaaccacgc
     2221 cgcgaatatc ggcaggccaa tgaacgcagg aagcaaccac gtggaggcgt atcggacgag
     2281 tttgtcatga gtctcgccgg caggttctct ggacgccatc aggagagcat acaacccggc
     2341 aagagcaagg cacacgcagg tccggatgag cacgctcggg aaatacgacg ggttgaagaa
     2401 acctgtccag aagcctcccg tttccaccca tcttcccggg gtcagcatga aagccagaat
     2461 gccattgata acgacgaggc tgccccacgc cgccccggcg tacgcccagc cgatgaactg
     2521 atgcgttttc tggtccacgc gatcccagcc gtagtagtac agcagtgctg cggcaatctc
     2581 cacgaggaac agcacccact ccattgccca gccgaacacg aaattgtgga tcaacgcgga
     2641 ggtggcgtca gggtggacaa gaccgatgga aaaccagatg ccgacacccg tcactgctcc
     2701 gaacaccagt gtcatcagga cgaaaaacag cgtgttccgt ttcgcaaatg caatgtattc
     2761 gggtttcccc ttccgaattc cgatccgctc ggtccaggca agaaaaatcc cgcctccgat
     2821 ggcaaaatgc gaaacgaaca cgtggggaat agcgatcaat gcgatgacga gtcccccgcc
     2881 gagcagcggg acgtcccaga acgggtaatt cataagccag ctcccctccc tcgttcatga
     2941 ccggaacggc ttcgcggcgg aggcacgttc ctgcatgtgc ctccgccacg ctttcacggt
     3001 tatcaccgcg cgagcgcggt caccaccttt ctgattgcgc gggcaatgtc atcgctgtcc
     3061 gccgcggtga gtctgggact caaaccgatg tggatcgttc gcccgagata gtccagcgtt
     3121 tcgggacaca tatccttgct gtactccacg ctccccttgt aaacggaagg atgccacgga
     3181 ctccagttgg aggatcgtcc gcgcttttcc agaacgtacg tccagtttgg ataaatgtgc
     3241 cggtccggaa tctccttgct gaacattgtt ccgcacggta tcccttccgc attcagtgcc
     3301 tttgcgtatg tattcgcgat ttccgcatcc ggcgcaaaaa acatcaggca gaccgacgcg
     3361 tcacccgatt cgtcgggcac gtcctgaaga gtaatcccgg caagcccgcg aatctggttc
     3421 acgattcggt acttggcgtt gtgcaggcgt tcgacaatgc ctcggagcct ccccatttga
     3481 accagcgcga tcgctccgct cacttcgctg atgcggaatc cttctccagg aatcggcctt
     3541 atggccgtct cttccggctt ccagaacccg aaagcggaat cgtgcgccat gagaattctc
     3601 tcgaacacca gacggtcgcc ggtaaccacc atgccgcctt cgcctgcggt tatggttttc
     3661 gacatctgat agctgtagca cccgacgtcc ccgattccgc ccagagtttt gcccttgtac
     3721 ttgccgccgt tcgcctgtgc cgtgtcttcg atgactttga ttccatgttt cttcgcaatc
     3781 gcgcagatct cgtccatgcg acacggtgtt ccgcgcatgt ggacaggaat cacagctttg
     3841 gtaagcccgg taatctttcg ctcaagatcc tccggatcca gcgtcaaaga attgtccact
     3901 tccgcgatca ccggaaccgc tcccacggta accactgcgg cggcggtggc gatgtacgta
     3961 taaccgggca cgatgacctc gtcccccggt ccgatatcaa gcccgaccag agcacagatc
     4021 agcgccgcag tcccgctgtt gacggcgagg gcgtaatccc gtcccgtgaa ctcggcaacg
     4081 gctttttcga gtctcgcagt ttccgactcc tctttcgcga tgtaccgaaa taccctcttt
     4141 tcccgcaaca ctttcagcac cgcttcttct tcttccactc ccatctcgta tgcgcccaac
     4201 cacgagggcg gaagaggtgc ggtacgaaca ggcgtgcctc catcaatcgc caaacggtca
     4261 ctcataaaaa cctccctgtc ccgggcgtga atcgcggggt ttgccgttat cccgcgtatc
     4321 caatatactc cacactcaga ggcggacaca gggcaagttc tgacggaagc gcaggcttat
     4381 gccagcgaac tgggaaatcc gaaagaaggc gaaaccatgg cggaaataac gcagggaaca
     4441 gacccgggca tctgcatcgt ccgtttcagt cagggcgcca ggatttgcgg acaactggcg
     4501 gacctgctga accccgtaga cccagcgacc ttccaccagt accgcttgcc ggccagaact
     4561 ccggtggaac tgcacttcca cgatttcgac gaatactggt ggttcgtgga aggacacccg
     4621 accgttacgc tgcgcggcaa ggacggcagg atggtggaag agattctcca gccaccggat
     4681 atggtagcgt gcgttcgggg atttgaacac acgttatatg cggaccacga ccttgtgtat
     4741 taccagtttt ccagtgttcg gaccggcagc gaacgggaag gccatctatc acgggcgaag
     4801 taaccttaag cggggcccga ccactgtttc tccgctttgt cgagttccgc tctggtacct
     4861 atgtcaatcc acctgccttc aaaaacgtat gccgcaacct cggtgaccgt acgcatccac
     4921 tgaatgaggt ggcccgcttt gtccggaccg cggccctctt cgaggtaacg gcggacaagg
     4981 ggaacgtcct gtctgcgaag agcgtagcaa aggatgccta tcagagggct cgtcggcgtt
     5041 tccggtttct caacaaatga ggaaaccaaa cccttctcga tgtgcacttc gctgtagagc
     5101 gatacgagat ccagagagcc cacgtcgtag caccccagaa ctgaacgccc cgccgcgcgc
     5161 tggagatctc tcaaagggcg cagcgaaaag tcgaaaagat tgtcaccgcc aacgataagc
     5221 aggtcgtcgt caactgactg cgtttcgatg gcgaattgaa tatctccgat tgcgcccaga
     5281 cggtccgcgt ttgacgtcgt cccgtcgctc aacggcacca catcgaatgg ccgctgctgg
     5341 gtttttgccc actccacgta atcctggaaa aacttcgcat tcgtcacgac gtacgtcgtg
     5401 tcgacatctc ccagtgtttc gatgccgtcg aggacgtaat cgacgatcgg tcggccgttg
     5461 accttgagga gcggcttgga ggcctccagt gtaaccgggt ggaggcgggt ggcgtagcca
     5521 ccggccagaa ccagcgcttt cacgaacgct ccttttccgg caaaagaact cgcaccatca
     5581 ttcgccttcc tcgaatccgt ggcggcgtct gagttcctcc cgtttggggt ggcgtgccca
     5641 gtgggcgggc tcgggccatc ggtcaggcac agtgagtaat acactgtcgc cttccacgcg
     5701 cacctgaaac aacgtaagcg ggcccgctgt cgtcagcggg gccccgtcag tcacacgcca
     5761 gagatattga tgccgcgggc attccaccgc attggcgtga agcgtcccca atcccagcgt
     5821 gctgccggcg tgcgggcatc ggtcgtcaaa tgcgaagaat gcgtcaccaa ctcgacacaa
     5881 cgcgacccgg tgcccgtcca cctctatgat tccgaggctg ccgtcctgcc attcttccgt
     5941 cttcgcaaca acaacctcgt gggccaacgc cgcctccctt tcctggtata ccttccttct
     6001 cagtagataa ccgctcctcc gaccactgtc aaccgatgtt gcccgcgccc ggcaatcccg
     6061 agtatttttt tgttgcaaaa ggggaggcgt tcgtgcccga aacacctatt tcttcgaacg
     6121 acacactgcg aggcgtcctc gatcatgtca ccttccgcaa cgatgatacc gggtttacga
     6181 tcgcacggct cgtgcgtgag cctgacaagg tgcc
//
LOCUS       DAOTNG010000186         6169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10912_length_6169_cov_4.825646, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000186 DAOTNG010000000
VERSION     DAOTNG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10912_length_6169_cov_4.825646"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1124
                     /gene="argC"
                     /locus_tag="P5179_09440"
     CDS             96..1124
                     /gene="argC"
                     /locus_tag="P5179_09440"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRS95481.1"
                     /translation="MKRQVSVTIFGASGYTGAGLYGLCLRHPGVNVAALSADRHAGER
                     LSAVFPRMAGLTDRVMVSVEEAVEQANEVAFLCLPHEAAMAVVPRLLDKGCKVFDLSA
                     DFRMDTPDSYAAWYKVSHTAPELLGVKVYGLPEFHRERIREARLIAVPGCYPTSVQIG
                     LAPLLKHRLIDPRTIIVDAKSGVSGAGRKLSQRTHYVETNENIAPYNVGRSHRHVGEI
                     EQELSKFAGEAVRVVFSPHLTPMNQGILSTMYVDLTHPLGTSELLDLLRSEYSGERFV
                     RVLPHLPETAFVQNTNYADVSVHVVPDTGRAIVLCAIDNLVKGAYGQALQCMNIACGF
                     DEECGLPV"
     gene            1149..1916
                     /gene="argB"
                     /locus_tag="P5179_09445"
     CDS             1149..1916
                     /gene="argB"
                     /locus_tag="P5179_09445"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRS95482.1"
                     /translation="MLALVKIGGSTLDSPGLIGELAFDLARLGAGRTILVHGGGKDIN
                     RALDRLGQPTTFVDGLRVTDAAAVETVEAVLSAQVNKRLVRALLSAGARAVGISGVDG
                     ALLQARPYGDGRLGYVGAIEKVDPSVLVALLNAGLLPVVSPISVGGDGLAYNVNADHA
                     AADIAMAMKVDELVFVTDVPGVLVEGRTVPLLTGADAEKLIAIGQITGGMIPKVRSCV
                     EAVRRGVGRVHILAWDGAATISEHLAGVRSHGTVITD"
     gene            1969..3201
                     /locus_tag="P5179_09450"
     CDS             1969..3201
                     /locus_tag="P5179_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRS95483.1"
                     /translation="MHDDSQSASLPAGKGVSREVIRRSKEFLLQNYARADVVFERGEG
                     VYLFDAEGERYLDCVSGLAVNALGYGDADVLEALREQSRKLIHVSNLYYTIPAASLAE
                     ELVRHSSADRVFFCNSGTEANEACIKFARRWARTHYGENKTGLVAAFSSFHGRTMGSI
                     SLTGQEKYQAPFEPLVPGIQFARFNDIDHTVEQINDSTAAVFLEPVQAEGGVHPATQE
                     YLKAVRSACDRHDALLVFDEVQVGLGRTGRLFCHEHYGVEPDMLTLAKPLAGGLPIGA
                     ALLKQHVADAIQPGDHAATFGGGPLICAVALVVFRKIADPQFLEKVRNNATLFGGRLR
                     ALKEQYSAIREIRGMGMIWGVQFDEPVAPIVAEFRKRHILVCVAGPNVLRFLPPLVIG
                     EEELNHVADVFAEILEAR"
     gene            3239..4456
                     /locus_tag="P5179_09455"
     CDS             3239..4456
                     /locus_tag="P5179_09455"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRS95484.1"
                     /translation="MASEVKKVALAYSGGLDTSVIIPWLKETYGCEIVAVVADLGQGE
                     DELRGIEEKAIRSGASDCVVLDLKQQFIEGYIWPTLKAGAIYERKYLLGTSFARPIIA
                     KAQVQVARETGADALAHGATGKGNDQVRFEVTYAALAPDLKVIAAWRDPAWKIRSRED
                     ALDYAAAHNVPVTASRKSIYSRDRNIWHLSHEGGALEDPWQEPPRDVWVLTVHPEDAP
                     DVPQYVEIQFEAGIPVGINGVRKDSVSLLTELNALGAAHGVGRCDLVENRLVGMKSRG
                     VYETPGGTIIYAAHRELESLVLDRETLHYKDIIAQRYAELVYYGKWFTPLREAIDAFV
                     DATQRDMNGTVRMKLYKGSCTVAGVQSENSLYDEALATFGASSYQQSDATGFLKLFSL
                     PERTIAMLKQRKG"
     gene            4467..5846
                     /gene="argH"
                     /locus_tag="P5179_09460"
     CDS             4467..5846
                     /gene="argH"
                     /locus_tag="P5179_09460"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRS95485.1"
                     /translation="MTTGKLWDGRFSRATDQVMEEFNASIGFDIRLWEADIEGNLAQA
                     DALRQCGILTSEEWSAIVLGLQQVRQELAGGSYRPGPDTEDIHMAVERRLIEIVGPVG
                     GKIHTGRSRNDQVALDVRLYLRRAVGTLTELVSQLQAVVLKRAEAEVDAFLPGYTHLQ
                     PAQPVRLAHYLLAFFWMLDRDKGRLRDALRRADEMPLGSGALAGTAFPIDREFVARRL
                     GFARPTRNSMDAVSSRDHLTETVSAIAILMTNLSRLAEDWVVWSSPAFGFVELDDAYA
                     TGSSMMPQKKNPDSLELVRGKSGRVFGDLMALVTIQKGLAFTYGKDLQEDKEPLFDAL
                     ETADICLRVLKGVVETAVFNHERMRAAIEPGMYATDLADVLVRRGLPFRDAHRVVGEL
                     VGKAIARGCSLVELPWEEFAQASPLFKREDVSTLSPAASTDRRNIFGGTGRNAVVQQL
                     ALARASLKE"
     gene            5855..>6169
                     /locus_tag="P5179_09465"
     CDS             5855..>6169
                     /locus_tag="P5179_09465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95486.1"
                     /translation="MLRAARISDVAEMAQFINSFAQRNLMLPRPLNRMYENLRDYVVV
                     EEDGKVVGCGALHILWYDLAEIRSVAVREGFQHRGYGKAILNSLLDEARKLGVHQVFM
                     LVL"
BASE COUNT         1272 a         1613 c         2022 g         1262 t
ORIGIN      
        1 ggcacaattg cgggagagga caccgttctc gtcgtgtgtc gtacaccggc ggatgccaac
       61 catgtgcttc gccagcttca gcaggtactg gaaggatgaa gagacaggtt tctgtgacga
      121 ttttcggggc gtccgggtat actggcgcag ggctttacgg cctttgtctg cggcatccgg
      181 gcgttaatgt ggcggctctt tcagcggatc gacacgcggg cgaacgactt tcagcagtct
      241 ttccccgcat ggcggggctg accgaccggg tgatggtgtc ggtggaggaa gcagtggagc
      301 aggcgaacga ggtcgcgttc ctctgccttc cccatgaagc ggcgatggct gttgtgccgc
      361 gacttctgga caaagggtgc aaggtcttcg atttaagcgc cgatttcagg atggatactc
      421 ccgattcgta tgccgcgtgg tacaaggttt cgcatactgc cccggagcta ctgggcgtga
      481 aggtgtacgg gttgccggag tttcatcgtg agcggattcg tgaggccagg cttattgctg
      541 ttccggggtg ctatcccacg tcggtgcaga tcgggcttgc gccgctgctg aagcatcgcc
      601 ttatcgatcc ccgcacgatt atcgtggacg cgaaatcagg tgtttccggt gccggaagga
      661 aactcagcca gcggacacat tacgttgaaa cgaacgagaa catcgcgccg tacaatgtgg
      721 gccgatccca ccggcacgtt ggtgaaatag aacaagaact gagcaaattc gcgggcgagg
      781 cggtgcgcgt ggtgttctca ccgcacctga cgcccatgaa ccagggcatt ctttccacga
      841 tgtacgtgga cctgacacac cctcttggca catcggagct tctggatctt ctccggagtg
      901 aatactccgg cgaacgattc gttcgcgtgc tgcctcatct gcccgaaacg gcgttcgtgc
      961 agaacacgaa ctacgcggat gtatcagtgc atgtagtgcc ggacaccgga cgggcgatcg
     1021 tcctgtgcgc gattgataac ctggtaaaag gcgcgtacgg tcaggcactc cagtgcatga
     1081 acattgcctg cgggttcgac gaagaatgcg gccttccggt gtaagaaacg cgcagcgcga
     1141 ggaaaacgat gttggcgctc gtcaaaatag gtggttcgac actcgactcc ccggggctga
     1201 tcggagaact ggcgtttgac ctcgcgcgtc tcggcgcggg acggacgatc ctcgttcatg
     1261 ggggggggaa ggacataaat cgcgcgctgg accggctcgg acaaccgacg acgtttgtcg
     1321 acggcctgcg ggtgactgac gccgcggcag tagagacggt ggaagcagtg ctgtcggcgc
     1381 aagtcaacaa gcgattggtg cgtgcgctgt tgtcagccgg ggcacgcgca gtcgggatta
     1441 gcggcgtaga cggtgctcta ctgcaagcgc ggccctacgg cgatgggagg ctcgggtacg
     1501 tcggcgcaat cgagaaggtc gacccttcgg tgcttgttgc ccttctgaat gccgggcttc
     1561 tgcccgtggt gtcccccatc agcgtaggtg gagacgggtt ggcgtacaac gtgaacgcgg
     1621 accatgccgc ggccgacatc gcgatggcga tgaaggtgga cgaactggtg ttcgtcactg
     1681 atgtgcccgg agtgctggtg gaagggcgta cggttccgct cctcactgga gctgacgcgg
     1741 agaagttgat tgcgatcgga cagataaccg gtggcatgat acctaaagtg cgatcgtgtg
     1801 tggaagccgt tcggcgtggc gtcggccggg tccacattct tgcctgggac ggggcggcaa
     1861 ccatctctga acacctcgcg ggtgttcgat ctcatggaac agtgataacg gattgattct
     1921 ggcacctgtc gcgccacggc agatgcggat gctcaggagg ttggtgaagt gcatgatgat
     1981 tcgcagtcgg cgtccctccc ggcggggaaa ggcgtttccc gggaagtgat ccgaagaagc
     2041 aaggaattcc tcctgcagaa ctacgcgcgg gctgacgtcg ttttcgaacg tggggaaggg
     2101 gtctacctct tcgacgcgga aggggaacgc tatctcgact gcgtctccgg cctggccgta
     2161 aacgcgcttg gttacggaga cgctgatgtc ctggaggcat tgcgcgagca gagccggaag
     2221 ctcatccatg tctcgaatct ctattacacg attcccgcgg catcgctcgc agaagagctg
     2281 gttcggcatt cctccgctga cagggtcttt ttctgcaatt cgggaacaga agcgaacgaa
     2341 gcgtgtatca agtttgcgcg tcggtgggcc cggacgcatt atggggagaa caagacgggg
     2401 ttggttgcgg cgttcagttc cttccatgga agaaccatgg ggtccatcag tcttacgggg
     2461 caggagaaat accaggcccc gttcgaaccg ctcgtgccgg gcatccaatt tgcccggttc
     2521 aacgatatcg accacacagt tgaacagatt aacgacagca cggcggcagt ttttcttgaa
     2581 cccgtgcagg cagaaggcgg ggtgcacccg gcgactcagg aatacctgaa ggccgtgcgc
     2641 tcggcctgcg acaggcacga cgcgctgctt gtctttgatg aagttcaggt tgggcttggc
     2701 cgaacgggaa ggcttttctg ccacgagcat tacggagttg aaccggatat gttaaccctt
     2761 gccaagccgc ttgccggagg cttgccgatt ggcgccgctt tgctcaagca acatgtggcg
     2821 gatgcaatcc agcccgggga ccacgctgcc acgttcggtg gtgggccgct catctgtgcg
     2881 gtggctctcg ttgttttccg gaaaattgcg gacccgcagt tcctggaaaa ggtaaggaac
     2941 aatgccacgt tgtttggagg aaggttgcgg gcgctcaagg aacaatattc cgctatccgg
     3001 gagatacgcg ggatgggcat gatatggggt gttcagttcg acgaaccggt agcgccgatt
     3061 gtggcagagt tcagaaaacg gcatatcctc gtgtgtgttg ccggtcccaa cgtgttgagg
     3121 ttcctccccc cccttgttat cggagaagag gaactgaacc acgtcgccga tgtgtttgcg
     3181 gaaatcctcg aggcacggtg atacacgaaa gactgctggt gaatgaaagg aaagtgacat
     3241 ggcgtctgaa gtaaaaaagg tggctctggc gtactccggt ggtctcgaca cgtcggtaat
     3301 aataccctgg ctgaaagaga cgtatgggtg tgagattgtt gccgtagtgg cggacctcgg
     3361 tcagggagaa gacgaacttc gtggcatcga ggaaaaggcg attcgaagcg gagccagtga
     3421 ttgcgttgtt ctggacctga agcagcagtt catcgagggg tacatctggc cgacactgaa
     3481 agctggcgcg atttacgagc gcaagtatct gctcggaacg tcgtttgctc gcccgattat
     3541 cgcaaaggcg caggtgcagg tggcgcggga aaccggggcg gacgctctgg cgcacggcgc
     3601 aacgggtaag ggtaacgatc aggtccgctt tgaggttacg tatgcagcgc ttgctcccga
     3661 tctgaaagtg atcgcggcgt ggcgcgatcc tgcctggaag atccggtctc gtgaggacgc
     3721 tctcgattac gcggcggccc acaatgttcc agtcactgct tcccgaaaga gcatctacag
     3781 ccgcgaccgg aatatctggc atctttccca cgagggcgga gcactggaag atccgtggca
     3841 ggaacctcca cgagacgtct gggtactcac ggttcatccg gaggacgctc ctgatgttcc
     3901 tcagtacgtg gaaatccagt tcgaggccgg catccccgtg ggaatcaacg gtgtgcggaa
     3961 agactcggtg agcctgctca ccgagttgaa cgcgcttggt gcggcacacg gcgtgggtcg
     4021 ctgtgatctc gtggaaaacc ggctcgtggg aatgaaatca cgcggggtgt acgaaacacc
     4081 cggaggaacg atcatctacg ccgcacatcg ggagctggaa agccttgtgc tcgatcggga
     4141 aaccctccat tacaaggaca tcatcgccca acggtacgcg gaacttgtct attacgggaa
     4201 atggtttacc ccgctgcgcg aggcgattga tgcgttcgta gatgcgactc agcgtgacat
     4261 gaacggtacg gtgcggatga agctgtacaa gggtagctgc accgtcgcgg gagttcagag
     4321 tgagaactcg ctgtacgacg aagcgcttgc gacgtttggc gcgtcttctt atcaacaaag
     4381 cgacgcgacc ggtttcctga agctgttttc tcttcccgag agaacgatcg ccatgttgaa
     4441 acagagaaaa gggtgaacgg cttcgtatga ccacagggaa gctctgggac gggcggtttt
     4501 cgcgcgcgac cgatcaagtc atggaagagt tcaacgcttc catcggattt gacattcgcc
     4561 tctgggaagc ggatatcgag ggtaatcttg ctcaggcgga tgcgctgcga cagtgcggca
     4621 tcctgacaag cgaggaatgg tccgccatag ttctgggttt gcagcaggtg cgacaggagc
     4681 tggcaggcgg gagctaccgc ccgggaccgg ataccgaaga catccacatg gcggtcgaac
     4741 ggcggctgat agaaatcgtc ggccccgtgg ggggcaaaat ccacacgggc aggagccgga
     4801 acgatcaggt cgcactcgat gtgcgcctgt acctccgtcg tgcggtagga acgctgactg
     4861 aacttgtgtc acagcttcag gccgtcgtcc tgaaacgcgc ggaagccgaa gtggacgcct
     4921 ttctccccgg ttacactcat ctccagccgg cacaaccggt acggctcgcg cattatctgc
     4981 tggcgttttt ctggatgttg gaccgggaca aagggaggtt gagggatgca ctccggcggg
     5041 cggacgagat gccgctcgga tcaggggcac ttgcggggac ggctttcccg attgacaggg
     5101 agtttgtcgc ccgccgtctc ggttttgcgc gccccacgcg aaacagcatg gatgcagtca
     5161 gcagccgcga tcatctgacg gaaacggttt ccgcgatcgc catcctgatg accaacctca
     5221 gccgactggc ggaggactgg gtggtgtggt cgagtcccgc attcggtttt gtcgagctcg
     5281 acgacgcgta cgcaaccggc agcagcatga tgccgcagaa gaagaatccg gattctctcg
     5341 aactcgttcg ggggaaatca ggacgggtat tcggggatct gatggcgctt gtgaccatcc
     5401 agaagggtct cgcgttcacg tatggtaagg acctccagga ggacaaagag ccgcttttcg
     5461 atgccctgga aacggccgat atctgcctcc gagtcctgaa aggcgtggtg gagacagcag
     5521 ttttcaacca cgaaagaatg cgtgcggcaa tcgaaccggg aatgtatgca accgaccttg
     5581 ccgatgtgct tgtgagacgg gggctgccgt ttcgagatgc acatcgcgtt gtgggtgagt
     5641 tggtgggaaa ggccattgcg cgcgggtgca gcctggtgga actcccgtgg gaggagttcg
     5701 ctcaggcaag tccgctgttc aagcgagagg acgtttctac gctctcaccg gccgcaagca
     5761 cggacagaag gaacattttc gggggcacgg gtcgcaacgc ggtagtgcag caactggctc
     5821 tcgcccgggc cagtctgaag gagtgaacgg caagatgctt cgtgcagcgc ggatatcgga
     5881 cgtcgcagag atggcgcaat tcataaacag tttcgcacaa cgaaacctta tgttgccgcg
     5941 gccactgaac cggatgtatg aaaacctcag agactacgtg gtggtggaag aggacggcaa
     6001 agtcgtcggg tgcggagctc ttcacattct ctggtacgac cttgccgaaa tccgatccgt
     6061 cgcggttcgg gaagggtttc agcaccgggg ttacgggaag gccatcttga acagcctgct
     6121 ggacgaagcg cggaaacttg gcgtccatca ggttttcatg cttgttctt
//
LOCUS       DAOTNG010000187         6159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_10935_length_6159_cov_5.702818, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000187 DAOTNG010000000
VERSION     DAOTNG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10935_length_6159_cov_5.702818"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /gene="rnr"
                     /locus_tag="P5179_09470"
     CDS             <1..2186
                     /gene="rnr"
                     /locus_tag="P5179_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRS95487.1"
                     /translation="LSARAKPLSLVQLASAAHSTQDDPLFRQAVMALRREGTIYAGRG
                     QRYASAAGLGARLGTLRLAERGYGFVAVEGREDDLYIPAEDIGNALDGDTVLVVEQSP
                     RGRRNLGRGVVLGIAYRPERMIVGTFHKGVGFAWIRPFSAQLPDLQLVFLGAEGDEKA
                     PSEGDVITAVLEPLSLESSAPAARFGTALGKQGDPWVDVTAILHAHGIRLSFPAEAVR
                     EAQQVAERAAEEAGKRADLRGLETVTIDPSDAADIDDAISFSKDDAGRDVVGIHIADV
                     SHFAPEKGAIDKEAYLRGTSVYLPGKVVHMLPPQLAAGLCSLLPRQDRLAVSVLITLD
                     ENVLPVRVEILESVIRSCAKISYEEAQEALGGSARPDNPAFPHAGMLQRLADITGELA
                     RKRSRHGALDFDVPEVRVEVDAQGRVVALRRRERLDSHRVIEELMLLANSVIADHLSQ
                     KHYPLLYRVHAGPDPAKLQELAPLALSLGQVIAPDGRSPSVQDLQKLLDAIRGNPVAP
                     VLEALIIRSLPKAVYQPDNIGHFGLAMDAYTHFTSPIRRYPDLVVHRQLKRALNRTPP
                     CYSHEQLFTIGDHCSAAERTAETAEREAIRAKQAEYLAGRVGEEFDGTVSGVRKFGLF
                     VVLNDSLAEGFVPVDDLPADTYAFDPTSLTLEGRRSRMTFRFGDPVSVQVVRVDVGSR
                     RIDFLLVEAPHSHRTRGPRTKTLRRSSKRPSRPTPGKRRGGRPSR"
     gene            complement(2190..2759)
                     /locus_tag="P5179_09475"
     CDS             complement(2190..2759)
                     /locus_tag="P5179_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS95488.1"
                     /translation="MLKDLVRECRSTRRFKQHIPVTRDALVEMVDLARICASGSNKQA
                     LKYFVSADPEQNARIFPTLAWAGYLTDWPGPAEGERPAAYIIILGDTTISEGFGVDHG
                     IAAQTIALAAREKGLGTCIMGAIQRPKLQEILNIPSHLQILLVLAIGVPAEKIQLEPV
                     GPDGSIKYYRDENGVHHVPKRSLEEVLVN"
     gene            2953..3423
                     /locus_tag="P5179_09480"
     CDS             2953..3423
                     /locus_tag="P5179_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRS95489.1"
                     /translation="MVIDFSTFQDGIHAVDWAGEIPGVDLIYPEIVAAIQVRAKIHRL
                     QEVVTIRAALRAQLERPCDRCLRPAVLAIESSLYVVVKLRAGLSAAEMGDDGEVLITV
                     DREESQVDLTDHIRESLIVEVPMTILCDDDCKGICPRCGADLNEESCSCRRQAE"
     gene            3478..3660
                     /gene="rpmF"
                     /locus_tag="P5179_09485"
     CDS             3478..3660
                     /gene="rpmF"
                     /locus_tag="P5179_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRS95490.1"
                     /translation="MANPKHRTSKARTRSRRTHWKLTAPNVVKCSHCGAPKVAHRVCP
                     ECGYYGQRLAVAPREE"
     gene            3769..4788
                     /gene="plsX"
                     /locus_tag="P5179_09490"
     CDS             3769..4788
                     /gene="plsX"
                     /locus_tag="P5179_09490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRS95491.1"
                     /translation="MERVCTIAVDAMGGDFAPDEIVAGAVHAAQQGDGAIRVILVGQE
                     QAVKASLARFGEVPGVEIRHAPTTVGMAESVSNLRRKMNSSLAVAIKMQKTGEADAAV
                     SAGNTGAAMALSLLLLGRIPGVNRPAIASPMPTKKGVCTLLDVGANSSCEPLNLYQFA
                     VMGSLYHSFVYNVPNPSVGLLSIGEEASKGDELVQKTHSLLAASSLNFVGNVEGRDIL
                     SGTSDIVVCDGFTGNVVLKSAESAVQFITGVLKDAIKSSPLSMLGGLLIKRQFNKQKT
                     RLNYEEYGGAPLLGIDGITIICHGRSSARAIQNAIRVAATAARQKLNEHIKNKLSEAS
                     VDATS"
     gene            4775..5758
                     /locus_tag="P5179_09495"
     CDS             4775..5758
                     /locus_tag="P5179_09495"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRS95492.1"
                     /translation="MRHPERAFFKGLGAAAPTKVLSNHDIEKLVDTSDEWIRTRTGIE
                     RRHVADDDTNTLTLATEAGRKALFDAGVEPEEVDMIILGTLTPYIGFPATACLVQNAL
                     GARNAAAVDINAACSGFLYGLHLAQSLICSGANRNVLLIGAETMSRIVNWKDRNTCVL
                     FGDAAGAALLAPSAGNEDGAGVLGTYIRSDGVQPEMLCRIGGGTKPVAKEEENYLQMS
                     GKDVFKRAVTAMGDAAEQILRQTGFSGEDITLLIPHQANMRIIEATAKRVNIPMDKVV
                     VNIQEYGNTSAATIPVAMAEAKASGRMRSGDIVLMVAFGAGMTWGAALARM"
     gene            5770..>6159
                     /locus_tag="P5179_09500"
     CDS             5770..>6159
                     /locus_tag="P5179_09500"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRS95493.1"
                     /translation="MGIAFLFPGQGSQYVGMGADLFAEFPAARRTYEDADALLGWPVT
                     SMSFAGPAETLNDTRYTQPAVFVHSMAVFEILRQHGIRPDVVAGHSLGEYAALCASGA
                     LEFGAALRLVAARAAAMADVASRKPGTM"
BASE COUNT         1244 a         1685 c         1960 g         1270 t
ORIGIN      
        1 ccctttcagc gcgggccaag cctttgtcgt tagtgcaact ggcttccgca gcacactcga
       61 cgcaggacga cccattgttt cggcaggcag tgatggcctt gcgccgggaa ggtacgattt
      121 acgcggggag gggtcagcga tacgccagcg cggcgggcct tggcgcgcga cttggcacct
      181 tgcgtcttgc ggaacggggg tatgggttcg ttgccgtcga aggtcgggag gacgatctct
      241 acataccggc ggaagacata ggaaacgccc tggacggtga caccgtgttg gttgtcgaac
      301 agagcccgcg cggaaggcgc aatctcggtc ggggggtggt gcttgggatt gcttatcgac
      361 ccgaacgcat gatcgtgggg acgtttcaca aaggcgttgg tttcgcgtgg attcgcccat
      421 tttcggctca attgccagac ctgcaactcg ttttcctcgg tgcggaaggg gatgaaaaag
      481 cgccgtcgga aggggacgtg ataacggcag ttctggaacc gttgtccctg gagtcgtctg
      541 cgcccgctgc gaggttcgga acagcgctcg ggaaacaggg cgacccgtgg gtggatgtca
      601 ccgccatatt gcacgcccac ggaatacggc tttcgtttcc ggccgaagcc gttcgtgaag
      661 ctcaacaggt tgcggaacga gctgcggagg aggcagggaa aagagccgat ttgcgtgggc
      721 tggaaacggt aacgattgat ccttcggatg ccgcagacat cgacgacgcg atatcgtttt
      781 caaaagacga tgccgggcgg gatgtcgtag gaattcacat cgcggacgtt tcccatttcg
      841 cgcctgaaaa gggtgctatc gataaggaag cgtatctgcg cgggacaagc gtctatctgc
      901 ctggcaaggt ggtacatatg ctgccgccgc agcttgctgc gggcctgtgc agcctgttgc
      961 cgcgtcagga tcgcctcgca gtcagcgttc tgatcacgct ggacgaaaac gttctgccgg
     1021 ttcgggtgga gatcctcgag agcgtgattc gctcctgtgc aaaaatctct tatgaagaag
     1081 cgcaagaggc gttgggcggg tcagcacggc cggacaaccc tgcgttcccg cacgcgggaa
     1141 tgctgcaacg gcttgccgac atcacgggtg aactggcgcg gaagcgatcg cgtcacggag
     1201 cgctggactt cgatgttccg gaggtgcgcg tagaagtcga cgcacaggga agggttgttg
     1261 cgcttcgccg gcgggaacgt ctggacagcc atcgtgtgat cgaagagctg atgttactcg
     1321 cgaacagcgt tatcgccgac catctgtcac aaaaacatta ccccctgttg taccgtgttc
     1381 atgcaggtcc cgacccggcg aaacttcagg aactcgctcc tctcgcgctg tcgctcgggc
     1441 aggtgattgc gccggacggc cgatctcctt cggtgcaaga cctgcagaag ttgctggatg
     1501 ccatccgtgg gaacccggtg gcgccagtgc ttgaagcttt gatcatccgg agcctgccga
     1561 aggcggtgta ccaacctgac aacatcgggc atttcggcct tgcgatggat gcatatacgc
     1621 acttcacgtc gccgatcaga cggtacccgg atctggtcgt ccaccgccag ctcaaacggg
     1681 cactgaacag aaccccgccc tgttattcgc acgagcaatt gttcaccatc ggtgaccact
     1741 gctccgcagc ggaaagaacc gcagaaaccg cggaacggga agccattcga gcgaagcagg
     1801 cggaatacct cgcggggcgc gtgggggagg agttcgacgg gacggtctca ggggttcgga
     1861 agttcgggct gtttgtggtc ttgaatgaca gtctcgccga gggatttgta ccggtcgacg
     1921 acctccctgc ggacacgtac gcgttcgacc ccacgtcgct tacgctggag ggaagaaggt
     1981 cgcggatgac cttccgtttc ggtgatccgg tgagcgtcca ggtcgttcga gtggatgtgg
     2041 gatctcgacg gatcgatttt ctgcttgtcg aagcgcctca ctcgcatcga acgcgaggac
     2101 cgcgcacgaa gacgctgcgg agatcgtcga aacgcccatc ccgtcccacg ccgggaaaaa
     2161 ggcgcggggg tcggcccagc cgctgaccgc tagttgacca aaacctcctc caggctcctc
     2221 ttcggaacat ggtggacgcc gttttcgtca cggtaatatt tgatgcttcc atcgggaccg
     2281 accggttcca gttggatctt ctccgcaggc acgccgatgg cgagcaccag aagtatctgg
     2341 aggtgcgaag ggatattcag tatctcttgc agcttcggcc tctggatggc gcccatgata
     2401 caggttccga gccccttttc ccgggccgcg agagcgatcg tctgggcggc aattccgtga
     2461 tcaacaccaa agccttcgct tatggtggta tcgccgagaa tgatgatgta tgctgcgggg
     2521 cgctccccct ccgccgggcc cggccaatcg gtgaggtagc ctgcccacgc aagggtgggg
     2581 aaaatgcggg cgttctgttc cgggtccgcc gacacgaagt atttgagcgc ctgcttattg
     2641 cttcccgagg cgcagatgcg cgcgaggtcc accatctcca ccaaggcgtc acgggttacg
     2701 gggatgtgtt gtttgaagcg acgcgtactg cggcattcac gaacgaggtc tttcagcatg
     2761 tgaacggccc tttccacgtg gagtgaattc tggagtatta cggaacaaag atgcaacaag
     2821 cggtttcgcg tgacaacgac cccaaccagg caccccgcgg tgtgcacaaa gtcccgacag
     2881 tgccgcgcgc gttgacttcc tcggtattgg aatgtattat aattagtctg tcttcaccgc
     2941 aagcgggata tcatggttat cgacttttct acgttccagg acgggattca cgccgttgac
     3001 tgggctggtg aaatccccgg cgtggatctc atctatcccg aaatagtcgc ggcgatccag
     3061 gttcgcgcga aaatccaccg cctgcaggaa gtagtcacga tccgcgctgc gttgagggct
     3121 caactggagc gtccttgtga tcgatgccta agacctgcgg tcctggccat cgaatcttcg
     3181 ttgtacgttg ttgtcaaatt gcgggccggg ctttccgcgg cggaaatggg agacgacgga
     3241 gaagtcctga taactgttga tcgtgaagaa tcccaagtgg atttgactga ccatattcgg
     3301 gagagtctga tcgtcgaggt accgatgacc attctgtgcg acgacgactg caaaggaatc
     3361 tgtccccggt gcggggccga tttgaatgag gaatcctgtt cgtgtcggag gcaggccgaa
     3421 tgacctgctt cttcgcgcga tggattgata tccaaattcc cggtaaggag tgattctgtg
     3481 gcaaacccaa aacatcgtac ttcaaaagct cgcacgcgtt cgcgccgcac ccattggaaa
     3541 ctcaccgcgc ccaatgtggt gaagtgcagc cattgtggcg ccccgaaagt tgctcaccgt
     3601 gtgtgtccgg aatgtggtta ttacgggcag cgcctcgccg ttgctccgcg cgaggaatag
     3661 ctcgcctgcc acacgcggcg ccgcctcccg ggtggcgccg tttactttgt ccggatttcc
     3721 cgctgcccgc gttcaggcgc gttctacact cgaatttgag gtgttggcat ggaacgggtg
     3781 tgcacaatcg ccgttgacgc catgggtgga gatttcgccc cggatgaaat cgttgccgga
     3841 gctgttcatg ccgcgcaaca gggcgacggg gcgatcaggg tcattcttgt cgggcaggag
     3901 caggccgtaa aggcatctct ggcgcgattt ggagaggtgc caggggtcga gatccgacac
     3961 gccccgacca cagtcgggat ggcggaatcc gtcagcaacc tccgtcgcaa gatgaactcc
     4021 tcgctggcgg ttgccatcaa gatgcagaag acgggagagg cggatgccgc ggtcagtgcc
     4081 ggaaacacag gcgctgcaat ggcgctttcc cttctcttgc tcggccgcat tcccggcgta
     4141 aatcggcccg cgatcgcgtc tccgatgcct acgaagaaag gagtgtgcac ccttctcgac
     4201 gtaggcgcaa acagcagctg tgagccactg aacctctacc agttcgccgt gatgggcagt
     4261 ctctaccaca gtttcgtcta caatgtgccg aatccatctg tggggcttct cagcatcggg
     4321 gaggaggcgt cgaaaggtga cgagctggtt cagaaaaccc attcgcttct tgcagcctca
     4381 agcctgaatt tcgtagggaa cgtggaaggg cgcgacatac tgagtggaac gtcggacata
     4441 gttgtgtgtg acggcttcac tggaaatgtt gttttgaagt ccgcggaaag cgcagtacaa
     4501 ttcataaccg gcgttctgaa ggatgcgata aaatcatcgc cgttgagcat gctcggtggg
     4561 ctcttgatca agagacagtt taacaaacag aaaactcgac tgaattatga agaatatggc
     4621 ggtgctcctc ttttgggtat cgatggtatt actattatct gccacggtcg ttcgtcggcg
     4681 cgtgccatcc agaatgccat tcgggtggcc gcgaccgcgg cgaggcagaa actgaacgag
     4741 cacatcaaaa acaaactgtc ggaagccagc gtcgatgcga catcctgaaa gagcgttttt
     4801 caaagggctg ggtgccgccg ccccgaccaa ggtgttgagt aatcatgaca tcgagaagct
     4861 cgtagatacc agcgatgagt ggattcggac caggactgga atagagcgaa ggcatgtcgc
     4921 ggacgacgac accaatacgt tgactctcgc gactgaagcg ggcagaaaag cgcttttcga
     4981 tgcaggcgtt gaacctgagg aagtcgatat gatcatcctc gggacgttga cgccctacat
     5041 tggttttccg gctacggcct gccttgttca gaacgcgttg ggtgcccgca acgcggctgc
     5101 cgtggatatc aatgcggcgt gttcaggttt cctgtacggt ctccacctcg cgcaaagcct
     5161 catctgtagc ggggccaatc ggaacgtgct actcatcggc gcggagacga tgtctcgcat
     5221 cgtcaactgg aaggacagga acacgtgcgt cctttttggc gatgcggcag gggcagccct
     5281 tctcgcgccc agtgccggaa acgaagatgg cgcaggcgtg cttggcacgt acatacgctc
     5341 ggacggcgtg caaccggaga tgctctgtcg tatcggcggc gggacgaagc cggtcgcgaa
     5401 ggaagaggaa aactatctcc agatgtccgg taaggatgtc ttcaagcgtg cggtgacggc
     5461 gatgggtgac gcggcggagc agattcttcg gcagacgggc ttctccgggg aagatatcac
     5521 gctgctcatc ccccaccagg cgaatatgcg cataatcgag gctaccgcga aacgtgtgaa
     5581 tatccccatg gacaaggtcg tagtgaacat acaggagtac ggaaacacct ctgcggccac
     5641 gattcccgtg gcgatggccg aggccaaagc ctcggggcgg atgcggagcg gagatatcgt
     5701 gcttatggtt gcgtttggcg ccggaatgac ctggggcgcg gcgctggctc gaatgtaggt
     5761 acggaacaga tggggattgc gttccttttt cccggccaag gctcccaata tgtgggaatg
     5821 ggtgcggatc ttttcgcgga gtttcccgcg gcgcggcgaa cgtatgagga cgcggatgcc
     5881 cttctgggtt ggccggtgac atcaatgagc ttcgcgggcc ctgcggaaac actgaacgac
     5941 acccggtata cccagcccgc ggtgtttgtc catagcatgg ccgtgttcga gattctgcgg
     6001 caacatggta tccgccccga tgtggtggca gggcacagcc tcggcgaata cgcggcactg
     6061 tgcgcctcgg gcgcgctgga gttcggggcg gcgttgcgcc tcgtcgcggc gcgagctgct
     6121 gcgatggccg atgtggcgtc ccggaagccc gggacgatg
//
LOCUS       DAOTNG010000188         6093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11073_length_6093_cov_5.614276, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000188 DAOTNG010000000
VERSION     DAOTNG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_6093_cov_5.614276"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="P5179_09505"
     CDS             complement(<1..864)
                     /locus_tag="P5179_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRS95494.1"
                     /translation="MYRDVPPTFSLPELERRILAFWKEHDTFNKLRRKNENGPRWSFI
                     DGPITANNLMGVHHAWGRTYKDAYQRYHSMLGQHLRYQNGFDCQGLWVEVEVEKELGF
                     KSKRDIEEYGIDRFVEACKARVIKFSKRQTEQSIRLGYWMDWDNSYYTMSDENNYTIW
                     HFLKKCHDRGFIYAGHDVMPWCPRCGTAISDQEIVTEGYQELTHTSVTAKFPLVGRPG
                     ESLLVWTTTPWTLTANVAAAVHPDLTYLAVKQGDETFYLAKACAERTLKNEYAVLREL
                     PGSELLGWAYSG"
     gene            complement(1016..1849)
                     /locus_tag="P5179_09510"
     CDS             complement(1016..1849)
                     /locus_tag="P5179_09510"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRS95495.1"
                     /translation="MNTPEPKLSEQIREALAVVQARSSLKPSVGIILGTGLGQLADEI
                     AVETVIPYGEIPHMPLSTVETHAGRLLIGHLGGKPVVAMQGRFHYYEGYTMKQVTFPV
                     RLMRAMGADTLLVSNACGGMNPAWEPGDIMIMTDHINLLGDNPLIGPNDDSLGPRFPD
                     MSEPYSRELIELCRAIAREHGIPVRAGVYVAVPGPNLETRAEYTFLRKIGADVVGMST
                     VPEVIVAIHGGMRVLGLSVITDRCLPDSLEPATIEQIIAMANKAQPGLTTLMRGVVER
                     L"
     gene            complement(1846..2133)
                     /locus_tag="P5179_09515"
     CDS             complement(1846..2133)
                     /locus_tag="P5179_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRS95496.1"
                     /translation="MQRDAEPLHRVTVHVQPRASKNAVGEMDAAGRLRVAVTAAPADG
                     QANEAVIALLADHFRVPRGAITLERGKTARTKIFALRGAQISTPAHDGNAT"
     gene            complement(2139..2960)
                     /locus_tag="P5179_09520"
     CDS             complement(2139..2960)
                     /locus_tag="P5179_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRS95497.1"
                     /translation="MNITPLDIRRNEFTRALRGYNREEVDSFLVMVADEVESLISEYR
                     DLSRKLEEQEKQLENFRMIERTLMETLITAQRTSDNMRETTQRECDAIKREAELKAQE
                     TIEKARLEAERLAMEARQRAHNVLEDARTKAHNAIEAALKQSDEIYQAAREEAMEIQT
                     ATKVMIERRDSFILQMRAFLGGQLEALDRFGHEELAAPPAVQEHEVPIITEEEAAAIA
                     ALDKELSAFAAATAPPEESVPVSEPPVREEAPAEEPPEDGAEVLVGRQLPRSREA"
     gene            complement(2998..3282)
                     /locus_tag="P5179_09525"
     CDS             complement(2998..3282)
                     /locus_tag="P5179_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRS95498.1"
                     /translation="MFQWQFFLNGVLRLLISLLNIYSIIIIVRVVASWLGADPYNAIM
                     NFLSRITDPFFEAIRRRLPSAMWNTGLDFSPLIAVLLIQVVTLLLESIRF"
     gene            complement(3282..3998)
                     /locus_tag="P5179_09530"
     CDS             complement(3282..3998)
                     /locus_tag="P5179_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRS95499.1"
                     /translation="MESAQDLHARIKLAVDHVREEIADAAVKSGRSPDEIALLAVTKT
                     RSPAEVNAAIGAGVTLLGENRVQEAEAKIPYVAGGAEWHLIGHLQTNKARLAARLFRM
                     VQSVDSVRLAEKLNDAVESGRSLDVLVEVNVSNEASKFGVAPGSVGELVEKLVELPAL
                     RVRGFMTIGAFVDDERLVRRGFRLLREIFDDTRRQFPSGQVDILSMGMTGDFRWAIAE
                     GSTMVRIGTALFGQRTQRGA"
     gene            4592..6004
                     /locus_tag="P5179_09535"
     CDS             4592..6004
                     /locus_tag="P5179_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRS95500.1"
                     /translation="MYFYWFIIMIFTLFQASAWVNMPSLPRMEDASLATTVREDLPDI
                     QKRDTLRVITQHSASSYFLFRGDERGLEYELARQFADMLDVHLQMVIPPTPEDMIPWL
                     MEGKGDIIAPGLLTAVPNHPVALRCLPYDSSYFVVVTRGSAAVASLDELAGRTVHAIP
                     SGPGYYALIDLNTQLGGAVTIAPAPSGWDYEMLLAALSKGEVEAVIAPWPLAKIERSY
                     HQNITIGPRIGQNEPVGWYVRPSANQLRDAVDVFMSRVQSSGITATLRAKYFDKGERA
                     ARYRAASARFLREGQISPYDRTMKLAAEEAGLDWLLVAAVAYEESGFAHDMESPMGAV
                     GLMQIMPETAASLGVADVLDPVQNIHAGTRYLRDQIAQFGSLEKRDAIAFGLAAYNVG
                     LGHVEDARKLAAEIGRDPNRWRGNVEAAMELLAHQRFYRRASSGYFRGSETARYVNAV
                     LARWETYERMMRRVGSQPAA"
BASE COUNT         1138 a         1838 c         1801 g         1316 t
ORIGIN      
        1 tcccgagtac gcccagccga ggagttcgga accgggaagc tcgcgaagta cggcatactc
       61 gttcttcagc gttcgttcgg cacaggcttt cgcgaggtag aacgtctcat cgccctgctt
      121 caccgcaagg taggtcaggt ctgggtgaac tgccgcggct acgttcgcgg taagtgtcca
      181 gggcgtggtt gtccacacaa ggagggattc acccggccgg cccaccagcg gaaatttcgc
      241 ggttacgctc gtgtgggtga gttcctgata cccttccgta acgatttcct gatcgctgat
      301 ggccgtgccg caacgcgggc accagggcat cacatcgtgc ccggcataaa tgaagccgcg
      361 gtcatggcac tttttcagga aatgccagat ggtgtagttg ttctcgtcgg acatcgtgta
      421 gtacgagtta tcccagtcca tccagtaccc gagacggatg gactgttccg tctgccgttt
      481 cgagaacttg atcacgcggg ccttgcacgc ttcgacaaac ctgtcgatgc cgtactcctc
      541 gatatctcgt ttggacttga atccgagttc cttctcgact tcaacctcaa cccacaaccc
      601 ctggcaatcg aagccgttct ggtagcggag gtgctgcccg agcatggagt ggtagcgctg
      661 ataggcgtcc ttatacgtgc gcccccacgc gtggtgcacg cccatcaggt tgtttgcggt
      721 gatagggcca tctatgaatg accagcgggg accgttctcg ttcttccggc ggagcttgtt
      781 gaaggtgtcg tgttccttcc agaatgcgag gatgcggcgt tccagttctg gcagcgagaa
      841 tgtcgggggt acgtcacgat acatgagttc tttcgtcttt ctccacaaat ctgtgataca
      901 ggatgatcca aacggaacac gcggaaccta acccggtgag gacctgtggc tgaggcccac
      961 ggtcccttcg aagcggcgtc gccccgatcc cgggcgctcc caatgcggtt ccggattaca
     1021 gtctttccac cacgccacgc atgagtgtgg tgagaccggg ttgtgctttg ttcgccatgg
     1081 cgatgatctg ttcgatggtc gcgggctcca gtgaatccgg caggcaacga tcggtgatga
     1141 ccgacaggcc aaggacacgc atgccgccgt ggatcgccac gatgacttcc ggaaccgtgg
     1201 acatgccgac gacatcagcc ccgattttcc gcaggaacgt gtattccgcc cgcgtttcca
     1261 gattgggacc ggggactgcg acgtacaccc cggcgcgaac cggtatgccg tgttcccgcg
     1321 cgattgctcg gcacagttcg atcagttcgc ggctgtacgg ttccgacata tccgggaaac
     1381 gggggccgag ggaatcgtca ttgggcccga taagcgggtt atcccccaga aggttgatgt
     1441 ggtcggtcat gatcatgata tcgccgggtt cccatgcggg gttcatacct ccacacgcat
     1501 tggatacgag cagggtatcg gctcccatcg cccgcatgag gcggacgggg aacgtcacct
     1561 gtttcatcgt gtagccttcg tagtaatgga agcgcccctg catggccacc accggtttcc
     1621 cgccgaggtg cccgatcaga agcctgcccg cgtgagtctc caccgtcgaa agcggcatgt
     1681 gaggaatctc gccgtacgga atcaccgttt ccacggcgat ctcgtccgca agttgcccga
     1741 ggccggtgcc aagaatgatg ccgaccgatg gtttcaggga ggaacgcgcc tgaacgacgg
     1801 caagggcctc acgtatctgc tctgacagtt tcggttctgg cgtgttcatg ttgcattccc
     1861 gtcatgtgcc ggcgtgctga tttgcgcgcc tctgagtgcg aaaatcttcg tacgcgcggt
     1921 cttcccgcgc tccaacgtta tggcgcctcg cggtacgcgg aagtgatccg caagaagcgc
     1981 gataaccgcc tcgttcgcct ggccatcggc cggcgccgcc gtaaccgcca ctctgagccg
     2041 tcccgcggca tccatctctc ccacggcgtt cttcgatgcc cgcggttgca catgcaccgt
     2101 gacacggtgt agcggctctg cgtcacgttg catccttctc aggcctcccg cgagcggggt
     2161 agctgccgcc ccacaaggac ttctgctccg tcttcgggcg gttcctcggc cggagcttcc
     2221 tcgcgaacgg gtggttccga tacgggaaca gattcttcgg gcggggccgt tgcggccgca
     2281 aatgcgctca gttccttgtc gagcgccgca attgccgcgg cttcctcttc ggtgatgatc
     2341 ggaacttcgt gttcctgcac cgcgggaggg gcagccagct cctcgtggcc gaaccggtcc
     2401 agcgcctcca actgtccgcc gaggaatgcc ctcatctgaa ggatgaacga atcccgccgt
     2461 tctatcataa ctttcgtggc agtctggatt tccattgctt cttccctggc tgcctgatag
     2521 atctcgtctg attgcttgag cgcggcttcg attgcgttat gcgctttggt gcgcgcatcc
     2581 tcgagaacgt tgtgcgcccg ttgccgtgct tccatggcaa gccgttccgc ctcaaggcgg
     2641 gctttttcga tggtttcctg cgctttcagt tccgcttccc gtttgatcgc atcacattcg
     2701 cgctgcgttg tctcgcgcat gttgtctgac gtgcgctggg cagtaatcag cgtttccata
     2761 agcgtccgct cgatcatgcg gaagttttca agctgttttt cctgctcctc gagctttcgg
     2821 ctgaggtccc gatattcgct tatgaggctt tcgacctcgt cggcaaccat gacgaggaag
     2881 ctgtccacct cctcgcggtt gtacccgcgc agcgctcgcg tgaactcgtt ccgtcgaatg
     2941 tcgagcggtg ttatgttcat cgctgctttc ctccagccca tgtgccgatg cggtggctca
     3001 gaaacgaatt gactccagca gaagcgtgac gacctgaatc aaaagcaccg caatcagcgg
     3061 ggaaaaatcc agtcccgtgt tccacatcgc tgagggcaga cgccgcctta tcgcctcgaa
     3121 gaaggggtct gtgatgcgtg aaaggaagtt catgatagcg ttgtacgggt ctgcgccgag
     3181 ccagctcgcg acgacacgca cgatgatgat gatgctgtag atgttcagca acgaaatgag
     3241 aagcctgagc actccattga ggaagaactg ccactgaaac atcacgcgcc cctttgcgta
     3301 cgctggccga acagagctgt tccaattcgc accatcgtgc tgccttccgc aatcgcccac
     3361 cgaaaatccc cggtcatacc catggagagg atatccactt gacctgacgg gaactgccgg
     3421 cgggtatcat caaatatctc gcgcaacaat cggaacccgc gacgtacgag cctttcgtcg
     3481 tccacgaacg cacctatcgt catgaagccg cgaacgcgaa gcgcgggcaa ctcgaccagt
     3541 ttttccacca gttccccgac ggatccagga gccacgccga atttggacgc ttcgttggat
     3601 acgttcacct cgacaaggac gtcgagagaa cgcccggact ccaccgcgtc gttcagtttc
     3661 tccgcgaggc ggacactgtc aactgattgc accatccgaa aaagcctcgc cgccagccgc
     3721 gccttgttgg tctgcaggtg cccgatcaga tgccactccg ctccgccggc gacatacggg
     3781 atcttggctt ccgcttcctg tacacggttc tcgccgagaa gcgtcacgcc cgccccgatc
     3841 gcggcgttaa cctccgccgg cgaccgcgtt ttcgtaaccg cgagcagcgc gatctcatcg
     3901 ggagagcgcc cggatttcac cgccgcgtcc gctatttcct cccggacatg atccacggcg
     3961 agcttgattc gagcgtgcag gtcctgtgcg gattccattc cggcagctcc gttgattcgc
     4021 ggtccgcaac gcgtacaccc cgaaacattt tccagacttt ataatgtacg ggcatgtatt
     4081 cggggcggca aatacggctg cccttcgcga tgggagtgtc gcaagtgaaa taggggaaaa
     4141 cgtgttatga acgtccggtc ctgagagcat ggaacgaggg catccgttca cgcgtctcaa
     4201 ctcccacaag aaccgaaagg cgccagtgcc gctcccgcag cctttcctct ggccggtcgg
     4261 ttgtcgcacc aagccggtga caggcgggcc cacgcgcgcg acatcactca agcgacatca
     4321 cgcaggtgaa agggtggcgg aatcgtgcag attctgcacg attccgctcc gggagccttc
     4381 tggacctttc cggccacaga agcgctaagt gcgcggaaat gcatccgatt tctttcgcgg
     4441 tatggcgaag tttttgcctc gtctggcacc acttttcgcc aggaacttag ctgctctccc
     4501 caaaatgccg tggcatggct cttgcttatg atactgcgcg taattcgtgt cgctctgctt
     4561 gcgcagtagt aacgaaagga acgcttaaca tatgtatttt tattggttta tcatcatgat
     4621 tttcacgttg ttccaggctt cggcgtgggt gaatatgccc tccctgccga gaatggaaga
     4681 cgcatccctt gcaaccacgg tgcgtgagga ccttccagat attcagaaac gtgatacgtt
     4741 gcgcgttatc acccagcatt ccgcatcaag ttacttcctt ttccgcggtg atgagcgggg
     4801 cctcgaatac gaactggcgc gccagtttgc cgacatgctg gacgtccatc ttcagatggt
     4861 gatcccgccg accccggaag acatgattcc ctggcttatg gaggggaaag gcgacatcat
     4921 cgcgccgggc cttttgaccg ctgttccgaa ccaccccgtg gcgctccggt gcctgccgta
     4981 cgatagttcg tacttcgtgg tggttacccg cggcagcgct gctgttgctt ctcttgatga
     5041 gcttgcggga cggaccgtgc acgcgattcc gagcgggcca ggatattacg cgctgataga
     5101 cctgaacacg cagctcggtg gtgcagttac aattgcaccg gcgccgtccg gatgggatta
     5161 cgagatgctc cttgcggcgc tttcgaaagg cgaagtggaa gccgtcatcg cgccgtggcc
     5221 attggcgaag atcgaacgga gctaccacca gaacatcact atcggtcccc ggataggcca
     5281 gaacgagcct gtcggctggt atgtccgccc gagcgccaac caactccgcg atgcggtgga
     5341 cgtcttcatg agcagagttc agtcctcggg aatcaccgcc accctccgcg cgaagtattt
     5401 cgacaagggc gagcgcgccg cccgttaccg tgctgcgagc gcccgattcc ttcgcgaagg
     5461 gcaaatatcc ccgtatgacc gtacgatgaa actcgcggcg gaagaggctg ggttggactg
     5521 gcttctggtg gctgcggtgg cgtatgagga atcggggttc gcccacgaca tggaaagccc
     5581 gatgggtgct gtcgggctga tgcagatcat gccggagacc gcagcatcgc tcggggtggc
     5641 tgacgttctg gacccggtgc agaacatcca cgccggcacg aggtacctcc gggaccagat
     5701 cgctcagttc ggatcgctgg agaaacggga cgcgatcgcg ttcggtctcg ctgcgtataa
     5761 cgttggactg gggcacgtgg aagacgcacg caagctggcg gccgaaatcg gccgtgaccc
     5821 gaaccgctgg cgcgggaacg tggaagcggc aatggagctt ctggcccacc agcgctttta
     5881 ccggcgtgcg tcgtcggggt atttccgcgg ctcagaaacc gcgcggtacg tgaatgcggt
     5941 gctcgcccgg tgggaaacct atgagcggat gatgaggcga gtgggcagcc aaccggcggc
     6001 ctgaggatcg ggggatagat cgagaacgcg aaagagcggg gcgcagaccc cgctcttttt
     6061 gttgtcgtgt tgctacagaa tgttccgcgc gcc
//
LOCUS       DAOTNG010000189         6076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11126_length_6076_cov_4.868959, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000189 DAOTNG010000000
VERSION     DAOTNG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11126_length_6076_cov_4.868959"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5179_09540"
     CDS             complement(<1..261)
                     /locus_tag="P5179_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS95501.1"
                     /translation="MENTSPQIVVVGSSNTDLVVRVPELPRPGETLLGLDFQIHAGGK
                     GANQAVAARRLGATVTLIANIGVDSFGEASIERFRAEGIRVGA"
     gene            complement(344..1429)
                     /locus_tag="P5179_09545"
     CDS             complement(344..1429)
                     /locus_tag="P5179_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRS95502.1"
                     /translation="MGEVRVAIIGAGNCASSLVQGVHYYQNASEDEFVPGLMHVNLGG
                     YHIKDIKFVAAFDIDKNKVGKDLSEALFSPPNNTIQFCQVPNLGVKVHRGMTHDGLGH
                     YLSEIIEKAPGPTDDIVKILKETKTDVVVNYLPVGSEEATKWYVEQVLAAGCGFVNCI
                     PVFIASQEYWQKRFEEKGLPVIGDDIKSQVGATITHRVLTTLFRDRGVRLEETSQLNV
                     GGNTDFLNMLERSRLQSKKISKTNAVTSMLDYDIGKDNVYIGPSDYVAWLKDRKWCYI
                     SMNGRTFGDVPLKLELKLEVWDSPNSAGVVIDAIRCCKLGLDNNLSGALIEPSAYFKK
                     TPPKQFPDSVARQNLEAWIKKYGNKKS"
     gene            complement(1632..3080)
                     /gene="murD"
                     /locus_tag="P5179_09550"
     CDS             complement(1632..3080)
                     /gene="murD"
                     /locus_tag="P5179_09550"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRS95503.1"
                     /translation="MKHYEPGQTWKGARVTVMGLGLFGGGVGAARFAAQQGAHVTVTD
                     LRPAEQLAESLDALRGLPIRYVLGRHEEADFESADIVIASPAVPLQSPFLARARNSGA
                     HLTTEILLFAERCRGNVIAVTGSNGKTTTTSLIGAILSLHDTRTVTGGNLGRSLLEIV
                     DQIAPGDPVVLELSSFQLEWLGPAHWYPHVAVITNLTPNHLDRHGTFASYVEAKRQIV
                     AHQPAEAHAVLNARDENLRALRSMTSGRVHWFDSANRVQDGTWLEEGDIVSSIGGRTH
                     HIMPASEVPLPGAHNLENVLAAVAATEIMGVQVPAIRKAVGSFRPVEHRLELVRTVNG
                     VRYFNDSIATTPESAICALRAFPERTLLLIAGGYDKKIPFDSLGAEIAERAAALITIG
                     TTSDKIRKAAVRAGFPEERIFPAGTLETAVQTAFSLAQPGQSVSLSPACASYDQFRNF
                     EERGRMFKKLVLELPENPVSRRDRRGKMNNSS"
     gene            complement(3077..3895)
                     /locus_tag="P5179_09555"
     CDS             complement(3077..3895)
                     /locus_tag="P5179_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRS95504.1"
                     /translation="MELRRHLVDKLRALTADFAYDGHDGTHQPLPSVPRRFRGHLGRG
                     REWAGTSGKCWAYTEDLRSTSRAANDLLPRLERACTENEQLAASFDAKIAALDIETCG
                     FTSAPVFMVGILTAGAETLSFHQAVARDYEEEPALLEWTRARLHDVGLVVTFNGTTFD
                     LPFLRERFRFHRLPELPQMRHLDLLPVCRRIWKGQFPNCRLQTLEERILGFVRKGDIP
                     GVEIPDVYHAFVSTGDSRHICRVAEHNRLDLISLLRILVEATRVRPKHEDDISR"
     gene            4051..4803
                     /locus_tag="P5179_09560"
     CDS             4051..4803
                     /locus_tag="P5179_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS95505.1"
                     /translation="MKAFLEWLGGISTFFGRTVAAAVRPPYEFREFVRQLDEIGTRSL
                     PLVGAAGAAIGVVLAMQTRSTLARFGADSLLPSMIAIAVVRELGPVITGLMVAGRIGA
                     GLAAEIGAMRVTEQIEALEVSAINPFRYLIAPRIAACIVAMPLLTAYCDFLALAGGYG
                     AASITMQTSVRLYFDASMQILEFVDVVPSLAKTAVFGFLIGTIGCYHGYSTRGGTAGV
                     GRAATFSVVLSSLMVILSDVLLVRISQTLFGG"
     gene            4890..>6076
                     /locus_tag="P5179_09565"
     CDS             4890..>6076
                     /locus_tag="P5179_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95506.1"
                     /translation="MVDVRPTPQQLAWQESELSMFLHFGMNTFTDREWGTGAEDPSQF
                     NPAKLDARQWARVAREAGFRYVILTAKHHDGFCLWPSRYTAHSVRNSPWADGKGDVVR
                     EFVDACSEFGLKAGLYVSPWDRHEPSYGDSDAYNRFFVNQLTELLTNYGEIAEVWFDG
                     ACGEGPNGRRQSYDWQAFYETVRALQPEALIAICGPDIRWVGNEDGYAAETEWSVRTP
                     DPSIHGSGTDTVWWPAECDVSIRPGWFWHPAEDDKVKSLPQLVDIYLRSVGRNSVLLL
                     NVPPNRDGLLPDPDVRRLRELRGYLDRAFARNLAEQAETTPSWGHAGDLASALRGCDP
                     QGWRVPTDSAEPVHIEFSFPSMVEFNTVVLRETIALGQFVERYRVEIQTAGGWREFST
                     GTT"
BASE COUNT         1202 a         1722 c         1807 g         1345 t
ORIGIN      
        1 tgctcccacc cgtatcccct cagctctgaa cctttcaatg gatgcttccc cgaacgagtc
       61 tacgccgata ttcgcgatga gtgtgacggt agctccaagg cgcctggcag ctaccgcctg
      121 attcgcgcct ttcccgccgg catgaatctg gaagtcaaga cccagcagag tctcaccggg
      181 ccggggcaat tcgggaacgc ggacaacaag gtcggtattg gaactgccca cgactacgat
      241 ctgaggactg gtgttctcca tggcagcggt acccccaaaa caaagggagg cggcatctgt
      301 acgaatgccg tctcccgata acgcttctcc attacgagct tcgtcaggac ttcttgttcc
      361 cgtacttctt tatccatgcc tcaagattct gccgtgcgac ggaatccggg aactgcttcg
      421 gcggcgtctt cttgaaatag gcgctcggtt caatcagagc cccgctcaag ttgttatcaa
      481 gcccaagctt gcagcaccga atggcgtcaa tcactacacc tgcggaattg ggtgaatccc
      541 acacctcgag tttcagctcg agtttgagcg ggacatcacc gaaggttctg ccgttcatgc
      601 tgatgtagca ccacttcctg tccttcagcc acgcgacgta atccgacggc ccgatgtaga
      661 cattgtcctt accgatgtcg taatcgagca tggacgtcac tgcgttggtt ttgctgattt
      721 tcttcgattg cagacgcgaa cgctcaagca tgttcagaaa atcggtgtta ccgcccacgt
      781 tcaactggct tgtttcctcc agacgtactc cgcgatcccg gaagagcgtc gtcagtacac
      841 ggtgggtgat ggtggctcca acctgcgact tgatgtcatc cccgatgacg ggaaggccct
      901 tttcctcaaa ccgtttctgc cagtactcct gcgatgctat gaacaccgga atgcagttca
      961 caaagccaca gcccgccgcc agaacctgtt ccacatacca tttcgttgct tcttcggaac
     1021 ccaccggcag atagttcacc accacgtccg ttttcgtctc tttgagaatc ttgacgatgt
     1081 catccgtggg gcccggcgct ttttcgatta tttcggacag ataatgtccg aggccgtcgt
     1141 gcgtcattcc gcggtgcact ttgacgccga ggtttggaac ctggcagaac tgaatggtgt
     1201 tgttgggcgg cgaaaagagt gcctccgaga gatccttccc gaccttgttt ttgtcaatgt
     1261 caaaagccgc gacgaacttg atgtccttta tgtggtaacc acccagattg acatgcatca
     1321 gaccggggac aaactcgtct tccgaggcat tctggtagta atgcacacct tgaacaagcg
     1381 aagaggcgca gttcccggct cctatgatcg cgacgcgtac ctcacccatg tgttgatctc
     1441 ctttttttac gaacctgcgc cgccggtatt cgagacgaac gttgaggtgc tgtgctcagc
     1501 gtgcggtgca gcagaaagcg ttgcatttga atcttgaatg gaagcggctc gctggatgca
     1561 agcaaagctc gcggcttcac aaatttctgg aaaaaagcat acagaacgca catgaatggt
     1621 ctgtgaatgt gtcatgaaga attgttcatc ttgccgcgtc ggtctcgtct cgaaaccgga
     1681 ttctccggca attcaagaac gagctttttg aacatccggc cccgctcttc gaaattcctg
     1741 aactggtcgt agcttgcaca tgccggagag agggaaacag actgcccggg ttgcgcgagt
     1801 gaaaatgccg tttgcactgc cgtttcgaga gtgcccgcag ggaagatgcg ctcttccggg
     1861 aagcccgccc gcacggctgc tttccggatt ttgtcggagg ttgtgcctat ggtgataagt
     1921 gctgccgcgc gctccgcaat ttcggctcca agactgtcaa atggaatctt tttgtcgtac
     1981 ccgccggcaa tcagcagcag agtcctttcc ggaaaggctc gcaacgcaca tattgccgac
     2041 tccggagtcg tagcaatcga atcgttgaaa tagcgaactc cattgacggt tcgaaccagt
     2101 tccaggcggt gttcaaccgg gcggaacgag ccgaccgcct tccggattgc aggcacctgg
     2161 actcccatga tttctgtcgc ggcgaccgct gcaaggacgt tttcgaggtt gtgcgccccg
     2221 ggcagcggca cttcagaagc cggcatgata tgatgcgtgc gccctccgat cgaggagact
     2281 atgtctcctt cttcgagcca ggtcccatcc tgcacgcggt tggcgctgtc gaaccagtgg
     2341 acgcgaccgg aggtcatgct tctcagtgcc cgaaggtttt cgtcccgtgc gttgagcact
     2401 gcgtgtgctt ctgcgggctg gtgcgctaca atctggcgct tcgcttcgac gtaggacgcg
     2461 aacgttccgt gccgatccag atggttcggg gtaaggttgg taatcactgc cacgtgagga
     2521 taccagtgtg ccggccccag ccattcaagc tggaagctgc tcaactcgag aacgaccggg
     2581 tcccccggtg cgatctgatc gacaatttcc agcaacgatc tgccaaggtt cccgccggtc
     2641 actgtccgcg tgtcatggag tgagaggatg gccccgatca gactcgtcgt ggttgttttt
     2701 ccgttggatc cggtcactgc aatcacgttt ccccggcaac gctctgcgaa aaggaggatc
     2761 tctgttgtga gatgcgctcc ggaattcctc gcgcgagcca gaaacgggga ctgcagagga
     2821 acagccgggc ttgcgatgac gatgtcggca ctctcgaaat cggcctcctc gtggcggccg
     2881 agtacatacc ggatcgggag accacgcagg gcatcaagag attccgcgag ctgctctgcc
     2941 ggcctcaggt cggtgacagt aacgtgcgcg ccctgctgag cggcgaatcg agcggctccg
     3001 acgccgccgc cgaacaggcc aagccccatc acggtaactc gtgcaccttt ccacgtctga
     3061 ccgggttcgt aatgcttcat cgggaaatgt cgtcctcgtg ttttggtcgg acccttgtgg
     3121 cttccacaag aattcgcagg agcgagataa ggtcgagccg attgtgctcc gcgacgcgac
     3181 aaatgtgcct cgaatcgcca gtgctgacaa acgcgtggta cacgtccgga atttccacac
     3241 ccggtatgtc tccctttctg acgaacccga ggatacgttc ctccagcgtc tgcagccggc
     3301 agttggggaa ttgccctttc cagatgcggc ggcaaacagg caacaaatcc agatgcctca
     3361 tctgaggcag ctcggggagg cggtgaaaac gaaaccgttc acgaaggaag ggcaggtcaa
     3421 aggtggttcc gttgaacgtc accaccaacc cgacgtcatg caaccgggcg cgcgtccatt
     3481 ccaacaacgc cggttcttct tcgtagtcgc gcgcaaccgc ctggtgaaag gataacgttt
     3541 ccgcgccggc tgtcagaatt ccgaccatga acaccggtgc ggaggtgaaa ccgcacgtct
     3601 cgatgtcaag cgcagcgatt tttgcatcaa acgacgcggc gagttgctcg ttttcggtgc
     3661 acgcgcgttc cagccgtggc aacaagtcat tcgccgcacg ggaagtggat cgcaggtcct
     3721 ccgtgtacgc ccagcatttc cccgaggtac cggcccattc cctgcctcgt ccgaggtgac
     3781 cgcgaaaacg ccttggaacg gagggcagcg gctggtgtgt gccgtcgtgg ccgtcgtacg
     3841 caaaatcggc cgtcaacgcg cgaagtttgt ccacgaggtg tctgcggagt tccatcgaac
     3901 caatctggtg gtgcgtgcgc tgttgaggac gacaaggaaa gatacggccg atcctcaact
     3961 ccagtcagcg ggatgcgagg cattgcaatg cggaggcaca attcaggtac ttcatggggt
     4021 cgcggttcta tttccgggga gaacgaagac atgaaagcct tccttgagtg gttgggtggg
     4081 atttccacat ttttcggaag gacggtcgcc gcggcggtgc gacctcccta tgaattccgc
     4141 gagtttgtac ggcaactgga cgagatcggc actcggtcgt taccgctggt aggtgcggcc
     4201 ggcgcggcga tcggggtggt acttgcgatg cagacccggt ccacgcttgc gcgttttggt
     4261 gcagattcgc tccttccgtc gatgattgcc atcgccgtgg tgcgcgaact cgggccggtg
     4321 attaccgggt tgatggttgc cgggcggatt ggtgcgggac tggccgcaga aatcggcgcc
     4381 atgcgggtta ccgagcagat cgaagctctg gaagtctccg caatcaaccc gtttcgttat
     4441 ctgattgcgc cgcgcatcgc cgcgtgcatc gtcgcgatgc ctctcctcac cgcgtattgc
     4501 gactttcttg cgctggcggg aggctatggc gccgcatcca tcaccatgca gacctccgtt
     4561 cggctctact tcgatgcgag catgcagatc ctcgaattcg tggacgtggt gccgagcctt
     4621 gccaagaccg cagtgtttgg ttttctgatc ggcacgatcg ggtgctatca cgggtattca
     4681 acccgcggcg gtaccgccgg cgtcggtcgg gcagccacgt tttcggtggt gctttcttcc
     4741 cttatggtga ttctttccga cgttctgctg gtaaggatca gccagacgct gttcggcggc
     4801 tgaacactga gtgcacagag ccgcagcgcg tacccgcgtg gcgttcgcgg ttgaggtgat
     4861 tcgcagatca agcgagaaaa ggaacggtta tggtggatgt tcggccgacg ccgcagcagc
     4921 ttgcgtggca ggaatctgag ctttcaatgt tcctccattt cgggatgaat acgttcaccg
     4981 acagagaatg gggaacgggc gctgaagatc cttcgcagtt caatcctgcg aaactggacg
     5041 cccgacaatg ggcgcgagta gcccgcgaag caggtttccg ttatgtgata cttacggcca
     5101 agcaccatga cggcttctgt ctctggccga gtcggtatac ggctcattcg gtcaggaaca
     5161 gcccttgggc cgatggcaag ggtgacgtgg tcagggagtt cgtggacgcg tgttcggagt
     5221 tcggcctgaa ggcagggttg tatgtgtcac catgggatcg tcacgaaccg tcgtacgggg
     5281 attctgacgc ctataaccgc ttttttgtga accaactgac tgagttactc acgaattacg
     5341 gggagattgc ggaggtctgg tttgacggtg cctgcggcga aggtccgaac ggcaggaggc
     5401 agtcgtacga ctggcaggca ttttacgaga ctgttcgagc tctgcagccg gaagccctca
     5461 tagccatctg cggcccggac atacgctggg tcggaaacga ggacggctat gccgccgaaa
     5521 ccgaatggag cgttcgaaca ccggaccctt cgattcacgg gagtggaacc gacacagtat
     5581 ggtggccggc ggagtgcgac gtgtcgatcc gaccgggatg gttctggcac cccgcggagg
     5641 acgacaaggt caagtctctc ccgcagctcg tggatatcta cctgaggtcc gtgggcagga
     5701 attccgttct gcttttgaac gttcccccca atagagacgg gctcctgccg gatccggacg
     5761 tgcgcaggtt gagggaactc cgggggtatc ttgatcgcgc gttcgcgaga aacctcgcgg
     5821 aacaggcgga gaccacgcct tcgtggggac acgccggcga cttagcttcc gcattgaggg
     5881 gttgtgatcc acaagggtgg cgggtgccaa cagactccgc ggagcctgtg cacattgagt
     5941 tctcgtttcc ctccatggtg gagttcaaca cggtcgtgtt gcgggagaca atcgccctgg
     6001 gccagttcgt agagcgttac cgcgtggaaa ttcagacggc gggtggctgg cgggagtttt
     6061 ccactggaac gactat
//
LOCUS       DAOTNG010000190         6048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11188_length_6048_cov_4.181545, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000190 DAOTNG010000000
VERSION     DAOTNG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11188_length_6048_cov_4.181545"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="P5179_09570"
     CDS             complement(<1..1804)
                     /locus_tag="P5179_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95507.1"
                     /translation="MEFVHHPGPYHVYLVPHSHIDVEWYWTYDKTRVWSRDILESAVQ
                     LLDTMPEEAFSQDQIPVLEFLAEQRRRQSGPLISQWLTSGRMEVLGGFVQPEVAEPDG
                     ECLIRQFVLGRKRAKEMFGVAQTVGWLIDTFGQVPQIPQILVGCGMDSYVFMRGVPRR
                     LGEVPTAFWCESPDGTRVLTYWMASGYTTSESTLLDSLKEEIAHSSSPMIMIGWGADV
                     TNPADAPAGKARETLIHAAAEEGLPLASVTVATPGAFFRALREQSGNLPTACEDFAPV
                     DDRFADLRGTYSNRTRHKIVNRKAEAALLAAEAWNALAVEFGSLDFRSELEKLWQRLL
                     FSQFHDTIGGSCTDQVYEKALKRCNTVVDLAEDITDQSLDVLAATADTGTFRYPVLVF
                     NPSCFPRTDIAEYEMVFREEPTNFRVVQADGKEVRHVIENPRGSKLATSGAIATAHVT
                     WLASDVPPLGFKVFAIEPIPGRVASTHTTTGSTSVENEWYRVEVDPTTGDVAQITDLS
                     TGYRCLDGAGNEIVAREEKNPNLEGCLGLTGEEERTSHTPAEVRVEVEKNNLFQRVVT
                     RRAFLGCQLVREVSLFRGIRRIHCRTRFSGFAGGD"
     gene            2098..3222
                     /locus_tag="P5179_09575"
     CDS             2098..3222
                     /locus_tag="P5179_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95508.1"
                     /translation="MNAALEPQSLSLAFLSDRELVSSMRNGSDDAWRIILHRYTPMLL
                     AAARTYARSCASRDRPGALADIASELYLFMAERVRGSLLRFYRGECEVRTWIYRIIGD
                     RRQIIKSFMVRADETRWRADTRFPKLMQDRPRIEQEVFRRLVWGKDALWIAQDLGMTE
                     AKAHELCETILEELANKSPRVYQRIMANRKALLRPLPIEAPQWRDDKERAALELRDES
                     PLPDETMENAALSRLLSRLRDVVAGLMKSLPEEDVRLLILVFDAGWSLTEIAERAPLV
                     GLPGLSARHQVDYRINRTLGHLAQHLCEHYGRWRDDTLPTPSAAEVTAALKGFLRETG
                     VAGFAGTGKSGEGNGFAHVFQPALEGNPPFPISGTKTQGP"
     gene            complement(3159..3863)
                     /locus_tag="P5179_09580"
     CDS             complement(3159..3863)
                     /locus_tag="P5179_09580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRS95509.1"
                     /translation="MGSRYVIPQNPGWSRLEGPATVLQRNHMVDQQICGRDIRDARVV
                     EAMRVLPRHVFVPPERRSDAYDDHPLPIGCGQTISQPYIVALMLELLETTGKERVLEI
                     GTGSGYQTGLLALLCHHVYTVERHQELSDSARQILSDLGFRNITFHCGDGSLGWEDYA
                     PFDRIIVSAATPSLSTQLVNQLDEGGVLVAPVGDRWEQSLKAVHKRSGKTKSTDHGPC
                     VFVPLIGNGGFPSNAG"
     gene            complement(3845..4390)
                     /locus_tag="P5179_09585"
     CDS             complement(3845..4390)
                     /locus_tag="P5179_09585"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRS95510.1"
                     /translation="MTPREYAHAQYRLTDITQAPVDLVPIIRHHQLRIVEVEVLDRAD
                     SRLQLSNTAGFIRIRRDIDHTRKRWEIARQIGLFVMLNEGERPRVRDTSVFASELLMP
                     AELVVRLYRNRREWLKNAMLVGMSATKRDMIRGLSWQFEVDDRAIAIRLDELHLGPGA
                     GPNKRVWSSSPGVIPRWEADT"
     gene            complement(4413..5585)
                     /locus_tag="P5179_09590"
     CDS             complement(4413..5585)
                     /locus_tag="P5179_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRS95511.1"
                     /translation="MRSALASLPERVLLGPGPSNVHPRVMRAISAPVIGHLDPAFISV
                     MEELKELLRKVFCTRNEFTIPFSGTGSAGMEAALANMIEPADSVIVCINGVFGTRMKE
                     IAERCGANVVTVEAPWGSAIDAGDVEKAMKVTPGVKLVAFVHAETSTGVLQPVDDICR
                     LAHEHGALVLADTVTSLGGAPVLTDDWGLDIVYSGTQKCLSCPPGLAPVTFNERALNV
                     IRGRKTKVQSWYLDATMLMRYWGEERVYHHTAPISMNYALCEALHLVLEEGLEARWKR
                     HIRNHRALVAGIEAMGLSMAVKEAIRAPMLNAVAVPDGIDEKRVRRSMLDGMNVEIGA
                     GLGPLAGKIWRIGLMGYSSRPENVLTCLSALNAALREQGKRCPDGLNAAIDAFQTD"
     gene            complement(5597..>6048)
                     /locus_tag="P5179_09595"
     CDS             complement(5597..>6048)
                     /locus_tag="P5179_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRS95512.1"
                     /translation="EQGPGLREETGFVNLKGKLPWPVEGRVTSRFGRRRDAATQTWTR
                     SRGIDIRVPKGTEVTAVGAGKIVMVDWFRGYGSFVIVSHGQNYYTLYAHLDEIHVQKN
                     EHVQQGQVIGLSGDSGTLGEEKLHFELLGGPQALNPLDWLQPRRTGL"
BASE COUNT         1184 a         1862 c         1738 g         1264 t
ORIGIN      
        1 catcgccgcc tgcaaatccc gagaaacgag tcctgcagtg aattcgccgg atcccacgga
       61 aaagcgagac ctctcgaacg agttgacaac cgaggaatgc gcggcgcgta accacccgtt
      121 ggaagaggtt attcttctcc acttccactc gaacttctgc cggcgtgtgg ctcgtgcgtt
      181 cttcctcgcc ggtgaggccc aggcatcctt ccagattcgg gttcttttcc tccctcgcga
      241 cgatctcgtt ccctgccccg tcgagacagc gataacctgt tgaaaggtcc gttatctggg
      301 cgacgtctcc ggttgtcggg tctacctcca cccggtacca ctcgttttcc acggaagtgc
      361 ttccagtcgt ggtgtgagtg gatgccacac gccccggtat cggttcgatg gcgaaaactt
      421 tgaaccccaa tggcgggaca tcgctggcca accatgtcac gtgtgcggtc gcaattgcgc
      481 ccgaggttgc aagcttggag cccctggggt tttcaataac gtggcgcacc tccttcccgt
      541 ccgcctgaac gaccctgaag ttggttggct cctccctgaa caccatttcg tactcggcga
      601 tgtccgtccg gggaaaacag gaaggattga aaacgagcac ggggtatcgg aaggtgcccg
      661 tgtcggcggt tgcggccagc acgtccagcg attggtcggt gatgtcctcc gcgaggtcca
      721 cgacggtgtt acacctcttc agtgccttct catacacctg gtcggtacaa cttccgccga
      781 tcgtgtcatg aaattgcgag aacagcaggc gttgccacag cttctccagt tcgctcctga
      841 aatcgaggct tccaaactcc accgcgagag cgttccaggc ttcggccgcg agaagcgctg
      901 cttccgcctt ccgattgacg atcttgtggc gcgtccgatt cgagtacgtt ccccgaaggt
      961 ccgcgaagcg gtcgtctact ggggcaaaat cctcgcatgc tgtcggcaaa ttacccgatt
     1021 gctcgcgaag cgcccggaag aaggcaccgg gcgttgcgac ggtgactgag gccagtggaa
     1081 gcccctcttc tgcggccgca tggatcaggg tctctcgcgc cttccccgcg ggcgcgtccg
     1141 cggggttggt gacgtccgcg ccccagccga tcatgatcat gggagaagac gagtgggcga
     1201 tctcttcctt cagggagtcc agaagagttg attccgacgt tgtgtacccg cttgccatcc
     1261 agtacgtcag aacgcgggtc ccgtccggcg actcgcacca gaatgccgtt ggcacttctc
     1321 ccagccggcg tggcacccct cgcatgaaga cgtaggagtc cattccgcat ccgacgagaa
     1381 tctgcggtat ctgcggaacc tggccgaatg tgtcaatcaa ccagccgacc gtctgcgcga
     1441 cgccgaacat ctctttcgcc cgtttcctcc ccagcacgaa ctgccggatg agacactcgc
     1501 cgtcaggctc tgctacttcg ggttgaacaa acccgccaag gacctccatt ctgcccgagg
     1561 tgagccactg tgaaatcagc ggtcctgact ggcgtcgtcg ctgttccgca agaaactcaa
     1621 gaacaggtat ctggtcttgg gaaaaggctt cttccggcat cgtatcgagc agttggactg
     1681 cgctctccag aatatcgcgc gaccacaccc gcgttttgtc gtaggtccag taccactcaa
     1741 cgtcgatgtg cgagtgaggg acgaggtata cgtggtacgg ccccggatgg tggacaaatt
     1801 ccatttgtgc gttccgtttg cggtgaagag tggaatggct tcagggcgac tgctttgcgg
     1861 tttcacgaaa taaggtaccc tcgcgccttc tgaagggaaa cgccacctgc gttggaactc
     1921 cccgaacggg aaaggatgtt gttacgcgaa gagaaggact gagcccgaat aacggcatct
     1981 gttccgtgtg atgtttctta cggggatttc gcggccgttc gaggccaaac catgcctggt
     2041 gctgcaacgg gcagctcgaa gtgatggccc gcaatgcgtg aaggtcgaaa ccacaccatg
     2101 aacgctgctc ttgaaccgca aagtctttca ctcgcattcc tttcagaccg cgagctcgtg
     2161 tcctccatgc ggaacggttc ggacgatgcg tggaggatca ttctccatcg ctacacaccg
     2221 atgcttcttg cagcggccag aacctacgca cgcagttgcg cctcacgcga ccgcccaggg
     2281 gcgctcgcgg atattgcatc agagctctac ctgttcatgg cggaacgggt tcgtgggagt
     2341 ctcctccggt tctatcgtgg cgagtgcgag gtgcgaacct ggatataccg gataatcggc
     2401 gacagacggc agatcatcaa atcgttcatg gttcgtgccg acgagacacg gtggcgtgca
     2461 gacacgcgct tcccgaagct catgcaggac cgccccagaa tcgaacagga ggtgttccgg
     2521 aggctcgtat ggggcaaaga cgcgctctgg atcgcgcagg acctcggcat gacagaggcg
     2581 aaggctcatg aactctgtga aacgattctc gaggaactcg cgaacaaaag cccccgcgtg
     2641 taccaacgaa tcatggcaaa ccgcaaggcg cttctacgtc cgctgccgat tgaagcgcct
     2701 caatggcggg atgacaagga gcgtgcggcg ctggagctgc gtgacgagtc tcccctgccc
     2761 gacgaaacca tggaaaacgc ggcattgagt cgcctccttt ccaggctccg ggacgtggtg
     2821 gcggggctca tgaagtcctt gccggaggag gatgtccggc ttctcattct cgtgtttgac
     2881 gctggctggt cgctgactga aatcgcggaa cgtgcgccac tggtcgggct gccgggtctg
     2941 tccgcccgcc atcaggtgga ttaccggatc aaccgtacac tcgggcacct tgcgcaacac
     3001 ctctgcgaac attacggcag atggcgtgac gatacgttac cgactccgag cgcggccgag
     3061 gtgacggcgg ctctgaaagg cttcctgagg gagactgggg ttgccgggtt tgcaggcacc
     3121 gggaaaagcg gcgaagggaa cggattcgca cacgtgtttc agccggcgtt ggaggggaac
     3181 cctccattcc cgataagcgg aacgaagacg caggggccat gatcggtaga cttcgttttg
     3241 ccggaccgct tgtgcactgc cttcagagac tgctcccacc ggtcgccaac cggggcaacg
     3301 agcacgcctc cttcgtccag ttggttcaca agctgggttg agagcgacgg agttgcggcg
     3361 gagacaatga ttctgtcgaa cggcgcgtag tcctcccacc cgagagaccc gtccccgcag
     3421 tggaaggtga tgtttctgaa accgaggtcc gaaagaattt ggcgagcaga atcagagagc
     3481 tcctggtgtc tctccacggt gtagacgtga tgacagagca gcgccagaag gcccgtctgg
     3541 taccccgatc ccgttccgat ctccagaact cgttccttcc ctgtggtttc cagtagttcc
     3601 agcatgagcg cgacgatata cggttgagat atggtctgcc cgcacccgat tggcagagga
     3661 tggtcatcgt acgcgtcgga acggcgctcc ggtggcacga acacgtgtcg tggcagtacg
     3721 cgcatcgcct ctactactcg cgcgtcccga atatcccgcc cgcatatctg ttggtctacc
     3781 atgtgattgc gctgaagaac cgtggcagga ccttcgaggc gagaccaacc ggggttctgg
     3841 gggatcacgt atcggcttcc catctcggga ttactcccgg gctactcgac cagactcttt
     3901 tgttcggccc tgcaccgggt ccgaggtgca attcatccag acgtatggca attgccctgt
     3961 catccacttc gaactgccac gacagcccac ggatcatgtc gcgtttcgtt gcgctcatcc
     4021 ccaccagcat ggcgtttttc agccactccc tgcggttgcg gtagagacgc acgacaagct
     4081 ccgcaggcat cagaagctca gacgcgaaaa ccgaagtgtc tcgaacgcgt ggccgttctc
     4141 cttcattcag catcacgaac agaccaatct ggcgggcgat ttcccaccgc ttgcgcgtgt
     4201 ggtcaatatc ccgccgtatt ctgatgaacc ccgcggtgtt cgacaactgg agtctgctgt
     4261 ctgcgcgatc gaggacctcg acttcgacga ttcgcagttg atggtggcgg atgattggaa
     4321 cgaggtccac aggcgcctgc gtgatgtctg tcagacgata ctgggcatgt gcatactccc
     4381 gtggcgtcat gcgtgtctcc caaatcgcaa aatcaatccg tctggaacgc gtcgatagca
     4441 gcgttgagtc cgtccggaca acgtttcccc tgttcgcgaa gcgctgcgtt cagagcggaa
     4501 aggcaggtca ggacattttc aggcctgctc gaatacccca tcaacccgat tcgccagatt
     4561 ttgccggcga gaggccccag ccccgctccg atttccacat tcatcccgtc caacatcgaa
     4621 cgacgcaccc ttttctcgtc gatgccgtcc ggaacagcca ctgcgttgag catcggtgcc
     4681 cgaatagctt cctttacggc catcgaaagc cccatcgctt cgattccggc gacaagcgcg
     4741 cggtggttcc ggatatgtcg cttccagcgc gcctcgaggc cttcctccag cacaagatgc
     4801 agcgcctcgc aaagcgcata attcatcgaa atcggcgccg tgtggtggta gacgcgctcc
     4861 tcgccccagt aacgcatcag cattgtggca tcaagatacc agctctgcac cttcgtcttt
     4921 cttccacgaa ttacattcag cgctcgctcg ttgaacgtga ccggcgccag tcccggaggg
     4981 cagctcaggc acttctgagt gccgctgtac acgatatcca gtccccagtc gtcggtgagg
     5041 actggtgctc cgccgagcga cgtgaccgtg tccgcgagga caagcgcgcc gtgttcgtgt
     5101 gcaagcctgc aaatgtcgtc caccggttgg aggactcccg tagatgtctc cgcgtgaaca
     5161 aacgcaacga gcttcacccc gggcgttacc ttcatcgcct tttccacgtc accggcgtcg
     5221 atggcgcttc cccacggcgc ttccaccgta acgacgttcg ccccgcagcg ctcggcgatc
     5281 tctttcatcc gtgtgccaaa cacaccgttg atacacacga tgacgctgtc cgccggttcg
     5341 atcatgttcg caagcgccgc ttccatcccc gccgaacccg tgcccgaaaa tggaatcgtg
     5401 aattcgttcc ttgtgcagaa caccttccgg agcaactctt tgagttcctc catcacgctg
     5461 atgaacgcgg gatcaaggtg gccgatcacc ggggcggaga ttgcgcgcat cacgcgtgga
     5521 tggacgttgg aagggccggg tcccagcaaa accctttcgg gaagtgacgc gagagctgaa
     5581 cgcatcggtg ctcctttcag aggccagttc gtcggggctg aagccagtcg agcgggttca
     5641 gtgcctgcgg accgccgaga agctcgaagt gcagcttttc ctcaccaagc gtgccgctgt
     5701 cgccggacaa tccgatcacc tggccctgct gaacgtgttc gttcttttgg acatggatct
     5761 cgtcaagatg cgcgtacaac gtgtagtaat tctggccgtg tgaaacgata acaaacgagc
     5821 cgtacccacg gaaccagtcc accatgacaa ttttcccggc tcccacggcg gttacttcgg
     5881 ttccttttgg aactcggatg tcaatgcctc tgctccgcgt ccacgtctgc gtggccgcat
     5941 ctctcctgcg accgaaacga gatgtaacgc ggccctccac cggccacggg agctttcctt
     6001 tgaggttgac gaatcccgtt tcctcgcgca aaccgggacc ctgttcga
//
LOCUS       DAOTNG010000191         6043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11195_length_6043_cov_4.808951, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000191 DAOTNG010000000
VERSION     DAOTNG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11195_length_6043_cov_4.808951"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="P5179_09600"
     CDS             <1..209
                     /locus_tag="P5179_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRS95513.1"
                     /translation="GKSIEGSLACLIACFVAGTAWAELPLACVWTGAVVATLAEALPL
                     RVNDNAIIPVSSGAAMAGLMFLFM"
     gene            753..1673
                     /locus_tag="P5179_09605"
     CDS             753..1673
                     /locus_tag="P5179_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRS95514.1"
                     /translation="MRVDYDVVDNKLVQTNKDDALATVFVNPDAQERTYLTETLEIDE
                     HTLTSALDPDELPRLEFEPEHTVLIFKNPKNYSATDELLLKAMSVGVFLFKDRVVVVA
                     SEDIPSLNIARFARVKTATDFLLYLLYRSIFHFREHLKVINMISDNLQDEIKSSMDNR
                     KLIDLFTLQKSMVYYLYAIQGNGALMDKLKSNAAKIRLSVEQLESLDDIIVENNQCFR
                     QAEIYANILASLMDARASIVSNNLNVLMKTLNLITIFIMLPTFVVSAFSMNVDLPWNL
                     KQQPYAFWIIMGLAFLSIVGGFIFWRHKKW"
     gene            1912..2694
                     /locus_tag="P5179_09610"
     CDS             1912..2694
                     /locus_tag="P5179_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95515.1"
                     /translation="MPIIDCHVHAGDKYGPVENVIAQMDANGVTHAVIVQHMGQTNNT
                     YLRQCIDKYPQRFWGICMVDLTLSNAPKILRSELKENGLHGVRLPAMSLSRRPDLWEM
                     AVECKAVISVLGLQPHDLGQVERLRAFLEKYPTARLKIEHLGYPEPSEPPPYATYQRV
                     MDLSQFKGVFLQFSSPYTQSKKAYPYEDLLPFIEMAKQHFAPNRIVWGSCYPPVEKYM
                     TYAQSLAWFGTLGLKDTHRDNALWQNAVRLMDPRKKKVVAET"
     gene            complement(2749..>3615)
                     /locus_tag="P5179_09615"
     CDS             complement(2749..>3615)
                     /locus_tag="P5179_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95516.1"
                     /translation="FDYVVAGLTGLDMPLHQTAIPDTAALKRDGGRNFMNEHAGPITT
                     WEEFEKYPWPDPGAPHATRLLEWYERNLPDDMCIIGGLTGHFAELITWLMGYETLCYA
                     LHDDRTLVKAIADRLYEFYEADVRHLLQFERVKMVWGSDDMGFKTGLLLSPDVMREYV
                     LSGHRRLAQMVHEAGRLYLLHACGNLSSIMDDLIFDVKIDAKHSFEDTIEDVRQVKHT
                     YGRHIALLGGIDLDFLCSASEERIRERVRDTLAVCQDGGGYCLGTGNSVANYIPLDNY
                     LAMVDEGMRWSW"
     assembly_gap    3616..3715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3716..3998)
                     /locus_tag="P5179_09620"
     CDS             complement(<3716..3998)
                     /locus_tag="P5179_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95517.1"
                     /translation="MNTFAGKERPHKPDWEAFVATVARRGTPQRVHHIELYQDGEIRD
                     AIAERYGLADGLDKADPYFAQKQYIAVQRFCGFDYVVAGLTGLDMPLHQT"
     gene            complement(4073..4951)
                     /locus_tag="P5179_09625"
     CDS             complement(4073..4951)
                     /locus_tag="P5179_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS95518.1"
                     /translation="MLSAVGVLLIWSGSGVFSHLATSGWNALQAAWWESLCGSLVALL
                     FLPATGLLRQVTALQPGDYVRLTTLGLLAHTGYYSFLFVGFASGHQVQTMALNYTWAV
                     FMVVFGGLINRAVPKWYQWVSVVLGVLGATLVVIPGSCSAADGTPGALAGLGAGLCFG
                     LFTPLSVRWSYDPRLVTSWMLFLSMLVFSLVMLLSGTSFSFRAHGMIGALYMGIGVDF
                     VGYALWQRANRRLHPWVVSVWACLIPLLNITLLTLVAGLKVPPPVYLGGSLIVAALVV
                     QNVFTLATRRTEPRGG"
     gene            complement(4999..5832)
                     /locus_tag="P5179_09630"
     CDS             complement(4999..5832)
                     /locus_tag="P5179_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95519.1"
                     /translation="MRTILLAAIAAACVVCAWSQASAAVIVTTSGEEIVCEIVRETDT
                     YFVVDHRGYRRYVLKTQVRSLDRRAGEGFNLGPEPLRNIEFSGGYRLWGRSQSRGIGA
                     SAISASYPLNENLLIQLGVEAGRGEASSSFEGGDLLPGPISWVGGMCGAQWQLQPWRV
                     RPFLDAGIGYFAMRHSVADSGVLWMKQAIAAIDGDTSFVYSEPMDGAFAVRLGVGALV
                     PLSGRFALTLRTSILVLHTGFTRQWYYPASAEQEEMTLRSSHGITLEMIHTTAGVQWA
                     F"
BASE COUNT         1354 a         1785 c         1606 g         1198 t
ORIGIN      
        1 gcgggaaatc gattgaaggg tccctcgcgt gtctgattgc gtgtttcgtc gcgggcacag
       61 catgggcaga attaccgctt gcgtgcgtct ggaccggtgc ggttgtcgcg acgttggcgg
      121 aagccctgcc ccttcgcgtg aatgataacg cgatcattcc ggtatcatcc ggtgcggcaa
      181 tggcgggcct gatgtttctc ttcatgtgag cttctgaatc ctctccggca cccggacgac
      241 aattctccac ctgatattga aattgcgaag acgatcccct gcggatcgcg gccctttcgc
      301 gcgatttgcg ggggtgcgtc cgccctttat tccaccgacg cggcaagcgc aacgaaccgg
      361 tgcccgaggg gaatgcgctc acgccgtggt cgttttgggc aacctgctcg cggcggtgag
      421 gagcctgcgg ggaacattga tcctttccaa tgcgaccaac acgccgcacg cctgtctcat
      481 tgcctgtttg agtccttcac ccgaaaaatc cggaccgggg tcgaccctgt ttcgatcgga
      541 taccggcaac cgggggactg cggtctgggt cgtcgcggaa aacggttgcg gtgcctaggt
      601 tcttgcgaag agcccgcggc ttttttcgtc ctccgcagac aacaagactg gtttcagggc
      661 gcgggtactg cgtaattttc agaagtatct cttccgggtt cgcggtttgc agaagccgcc
      721 tgaaacagcc tttggggcca tggagtgtca gtatgcgggt ggattacgac gtcgtcgaca
      781 acaagctggt acaaacaaac aaagacgacg cgcttgccac cgttttcgtg aatcctgacg
      841 cgcaagaacg aacctacctc acagaaacgc tcgagattga cgagcatacc ctcacatccg
      901 cgcttgaccc cgatgagttg ccgcgtctcg agttcgagcc ggaacacacc gtcctgatct
      961 tcaagaaccc caagaattac tcggccacgg atgagctgct actgaaggca atgtcggtcg
     1021 gtgtctttct tttcaaagac cgcgtagttg ttgtggcgtc ggaggacatc ccatcactga
     1081 acattgcgcg ttttgcgcgc gtcaaaacag cgacagattt tctcctgtac ctgctttacc
     1141 ggtccatctt tcatttccgc gaacacctga aggtgatcaa catgatttca gacaaccttc
     1201 aggacgagat caagtcatcg atggacaatc gaaagctgat cgatctgttc acactgcaga
     1261 aaagcatggt gtattacctg tacgcgatcc agggcaacgg cgcgttgatg gacaaactta
     1321 agagcaatgc cgctaagatc cggctgagcg tcgaacagct tgaatccctg gacgacatta
     1381 tcgtcgagaa caaccagtgt ttccgacagg cggaaatcta tgccaacatc ctcgccagcc
     1441 tgatggatgc gagggcttcc attgtaagca acaacctgaa cgtccttatg aagacgctca
     1501 acctgatcac gattttcatc atgctgccaa cgtttgtcgt gagcgcgttt tcgatgaacg
     1561 tggacctgcc gtggaacctg aagcagcagc catacgcgtt ctggatcatc atgggtttgg
     1621 cgttcctctc gatcgtaggt ggtttcattt tctggcgtca caagaaatgg tgactttcca
     1681 gttctctttt tcgacagacc gtctgccacg attccaaacc cgccgtcacc gccccacttc
     1741 tcatactgct ctgcacttct tataattacc tcaggatgcg gttgtcgttc gaggagcaac
     1801 gcctgccacg acgccgcctg cgggcacccc tgaagtccgg ttcacagcac caatcgtcgt
     1861 aacacaggaa gcgcagtatc gcgtttctgt tgcgtgtgca aagggttttc tatgccgatc
     1921 atcgattgtc acgtacacgc gggcgacaaa tacggccccg tcgaaaacgt catcgcccag
     1981 atggatgcaa acggcgtaac ccacgcggtg atcgtccagc acatgggcca gacgaacaac
     2041 acgtatctta gacaatgcat cgacaaatac cctcagcgat tctggggcat ctgcatggtt
     2101 gatctcactc tttcgaacgc tccgaaaatc ctgaggtcgg agctgaagga gaacggcttg
     2161 cacggtgtga ggttgcccgc gatgagcctt tcgcgtaggc cggatctgtg ggaaatggcg
     2221 gttgagtgca aagccgtgat cagcgtgctg ggacttcagc ctcatgatct ggggcaggtg
     2281 gagcggctga gggcgttcct cgagaaatac cctaccgcaa ggctgaagat tgaacacctg
     2341 gggtacccgg aaccgagcga accaccgccg tacgcgacgt accagcgagt gatggatttg
     2401 tcgcaattca agggagtatt ccttcagttt tcctcgccct atacacagag caaaaaggcg
     2461 tatccctacg aagatcttct acctttcatc gaaatggcaa agcagcactt cgcaccaaac
     2521 cgcatcgtct ggggttcatg ctacccgcct gttgagaaat acatgacgta cgcccagtcg
     2581 cttgcctggt ttggaacact cggattgaaa gacacgcaca gggataacgc attgtggcag
     2641 aatgctgtgc ggttgatgga ccctcggaag aaaaaggtag tggccgaaac ctgagacgag
     2701 cttgcagatc agaacaaggg gcgcgtttta cgcgcccctt tttcttcttc accaactcca
     2761 acgcatacct tcgtccacca tcgcgaggta attgtcgagg gggatataat tggcaacgct
     2821 gttccctgtg cccagacagt accctccgcc atcctgacag acggcaagcg tatccctcac
     2881 ccgctcacga atccgctcct cagaagcaga gcaaaggaaa tcgaggtcga tacctccgag
     2941 gagcgcgatg tggcggccat acgtgtgctt cacctgccgg acgtcctcga tagtatcttc
     3001 gaaactgtgc ttggcatcga ttttcacgtc gaagatcaga tcatccatga tcgacgaaag
     3061 attcccgcac gcgtgaagca agtagagcct tcccgcctcg tgcaccatct gcgcgagcct
     3121 ccggtgtccg gaaagcacgt actcgcgcat tacgtccggg ctgaggagaa ggccggtttt
     3181 gaaccccatg tcgtcgcttc cccacaccat cttcactctt tcgaattgaa ggaggtgccg
     3241 gacgtctgct tcatagaact cgtacaatcg gtcggcaatc gccttcacga gagttctgtc
     3301 gtcgtgcaat gcgtaacaga gagtctcata gcccatcaac caggttatca gttcggcaaa
     3361 atggccggtg agaccgccga tgatacacat gtcgtccgga aggttcctct cgtaccactc
     3421 gagaagacga gttgcatgtg gcgcgccagg atcaggccag gggtatttct cgaactcctc
     3481 ccatgtggta atcgggcctg cgtgttcgtt catgaaattc cttccgccat cgcgtttcag
     3541 cgccgccgtg tccggaatgg cggtctgatg aagcggcata tcgaggcccg tcaaccccgc
     3601 caccacgtag tcaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggtc
     3721 tgatgaagcg gcatatcgag gcccgtcaac cccgccacca cgtagtcaaa tccgcaaaac
     3781 cgctgcacgg cgatgtactg cttctgcgcg aagtacgggt ccgctttgtc gagaccgtcc
     3841 gcgagcccgt agcgttccgc aattgcatcg cgaatctcgc cgtcctgata gagctcaata
     3901 tggtggaccc gttgtggcgt gcctcgcctt gcaacagttg ctacgaacgc ctcccagtca
     3961 ggtttgtgcg ggcgttcctt ccctgcaaag gtgttcatcg tttcatcctc ggtcaaaaag
     4021 ggcggatccc aaaaagaaac tgcgtatcat cgttcgtcgc gccgacgtta actcaaccgc
     4081 cccgcggctc cgtccgccgt gtcgcgagcg tgaagacgtt ttgcacaacc agcgcggcca
     4141 cgatcagact gccgccgagg tacaccggag gcggcacttt caaaccagct accagcgtga
     4201 gcagcgtaat gttcaacaac ggaatgaggc acgcccacac cgagacaacc cacggatgca
     4261 ggcggcggtt tgcgcgttgc cacagcgcat accccacgaa gtccacgccg atacccatgt
     4321 aaagcgcacc aatcattcca tgtgcccgga aactgaacga cgtgcctgaa agaagcatga
     4381 cgagcgaaaa caccagcatg ctgagaaaca acatccatga cgtgacgaga cggggatcat
     4441 aagaccatct cacggataac ggggtgaaga gcccgaaaca aagccccgca cccagccccg
     4501 ccagcgctcc cggagtcccg tccgctgcac tgcaagaacc cggtatcacc accagtgttg
     4561 cgcccagaac cccgagtacc acggataccc actggtacca cttcggcacc gcccggttga
     4621 tgagcccgcc gaacacaacc atgaagactg cccaggtgta gttcaaggcc atcgtctgga
     4681 cctggtgccc tgacgcaaag ccaacaaaca ggaacgagta gtaacccgtg tgcgcaagca
     4741 atcccagcgt ggtcagccgc acgtaatccc ccggctgcaa ggccgtcacc tgtctcagca
     4801 aaccggttgc cgggaggaaa agaagggcga cgagtgaacc gcagagcgat tcccaccatg
     4861 ccgcctgcaa tgcgttccat ccgctggtgg caagatggga aaaaacgccg gaaccactcc
     4921 agatcagaag cactccgacc gctgataaca cggcctccgg tcgggatacg gaaacacggg
     4981 ataccccccc gtcggagttc aaaatgccca ttgaacgcct gcggtcgtgt ggatcatctc
     5041 cagtgtgatt ccgtgggatg atcgcaacgt catctcctct tgttcggcag aggcgggata
     5101 gtaccactgg cgcgtgaaac ccgtatgcag caccagaatg gacgtacgca gggtaagcgc
     5161 aaaccgtcct gacaaaggca cgagggcgcc cactccaagc ctgacggcga aggcaccatc
     5221 catgggctca gagtacacga aactcgtatc gccgtcgata gcggcgatgg cctgtttcat
     5281 ccagagtacg ccggaatcag caacggaatg gcgcatcgcg aagtacccta ttccggcatc
     5341 gaggaaaggc cgtacccgcc acggctgcaa ttgccactgt gcgccacaca tgcccccgac
     5401 ccacgatatc ggccccggga gcaggtcgcc gccctcgaac gaggacgatg cttcgccccg
     5461 gcccgcctca acgcccaact ggataagaag gttttcgttc agagggtaac tggcagaaat
     5521 cgccgaagcg ccaatcccac ggctttgtga cctcccccac aggcggtacc cgccggaaaa
     5581 ctcgatgttc ctcaacggct cgggaccgag gttgaacccc tcccctgctc tccgatccag
     5641 cgaccgaacc tgtgtcttca gcacgtatct ccggtaaccg cggtggtcga cgacgaaata
     5701 cgtgtccgtt tcgcgcacaa tctcgcagac aatctcctct ccgctcgtcg tcactatgac
     5761 tgccgcagag gcttgcgacc atgcacatac cacacacgcg gccgcaattg ccgccagtag
     5821 aatcgtacgc aaacggaatc tcccctggtc ttgggcaaaa ccggatatcg ttccgtttca
     5881 aatctcagca cggggatgcc gtggagcaat cggctggact gcagtcttcg cggaaatcag
     5941 aaggaaaatg ggcctgcaga tggcattgaa cggcggggag gtcacgacaa aggagaccgg
     6001 ggcatcggag gccgcctgac ggcaacgacc ttcattctgg tcc
//
LOCUS       DAOTNG010000192         6015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11237_length_6015_cov_5.286577, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000192 DAOTNG010000000
VERSION     DAOTNG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11237_length_6015_cov_5.286577"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1010)
                     /locus_tag="P5179_09635"
     CDS             complement(294..1010)
                     /locus_tag="P5179_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95520.1"
                     /translation="MSAGTTSGDFLKPSKKTPVRVTVRNPEREKAGQLTPKETEIPRL
                     AEEKAPQTRVVYSTRGYEYEAPFNYPNVNCELGRFGTGTGAHPDGMELDIPPFGAITI
                     EEAEPIIGVTVIGSPCIPPGTILVFGEPLEWKYGRSGVKFKQTNIWGEHLYRWGANPE
                     FEEGNRHAGLASVHFEIPDCRKVTVMGYGQLDVHVSPPEWTPGKGRQGLEQYEMPGED
                     WWNDAHYNVRTIRITVRVPA"
     gene            1195..2469
                     /gene="aroA"
                     /locus_tag="P5179_09640"
     CDS             1195..2469
                     /gene="aroA"
                     /locus_tag="P5179_09640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRS95521.1"
                     /translation="MKTHARPITPVSSLHATIRPPGSKSLTNRALLCAALAYGESEIS
                     NALVSDDSRYMISALRKLGVDLSVSADGTMIRVRGCSGRFPAKKADLFVGNAGTAMRF
                     LTAALTLGTGVYTVDGDERMRQRPILDLVYGLRALGADVLACNDPFPPVLIRAEGLDG
                     GSCEIDGTTSSQFVSAVLMAAPYARRDTVVTVKNGLVSAPYVGMTRHVMRAFGADVEE
                     TEPHVYHVAANSRYFGTTFAVEPDASAGSYFFAAAAITGGTIRVTGIGKNSVQGDRAF
                     VRLLEAMGCVVREGADWTEVTGKPLHGISADMADISDTAMTLAVVALFADSPTSIRGI
                     GNVRVKESDRIAAIATEARKLGAQVEEFEDGLRITPAPLRGAMIETYNDHRIAMSFAI
                     AGLRQPGVVISNPECVNKTYPGFFEELTKLGG"
     gene            complement(2542..2775)
                     /gene="rpoZ"
                     /locus_tag="P5179_09645"
     CDS             complement(2542..2775)
                     /gene="rpoZ"
                     /locus_tag="P5179_09645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRS95522.1"
                     /translation="MLLVDQLTRHGHSVYSIVIAAAKRARKINDWRIQRAKILGEETF
                     GPKPTVQALEEIANGTVKVVREDAGRQQQAISA"
     gene            complement(2832..3440)
                     /gene="gmk"
                     /locus_tag="P5179_09650"
     CDS             complement(2832..3440)
                     /gene="gmk"
                     /locus_tag="P5179_09650"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRS95523.1"
                     /translation="MQSRGFALVISAPSGTGKSTITKAVLEAESRCVQSVSATTRKPR
                     AGEVDGKDYFFVSLDEFERKKRDGELIEWAEFAGNFYGTPRAFIEKAKAHGDIAVLDI
                     DIQGAAQIRRTMPDAVTIFVAPPSLAALEQRLRARKTETEEAIAKRLARVPSELAAAK
                     YYDYLVWNDRVENAVQAILEVIRVESRRISRMDLEGDWLPKM"
     gene            complement(3456..4343)
                     /locus_tag="P5179_09655"
     CDS             complement(3456..4343)
                     /locus_tag="P5179_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRS95524.1"
                     /translation="MIRSMTGFGRGQSSVGGYCVTVEIRSVNSKFIEVSCRIPHALSS
                     LEQHVIEVVQERLGRGKVSVNVSLDNSAGNGTSTVREVIPNLPLAEAYKKAVETVRDH
                     LGMSADVDLAALVRMDGVLAVRDTQLESETAAALLDGALDAAIEELQTMRCKEGEALA
                     KDFLFRIDRLRELVSLVEARAPERVEEAKRKLTERISALLGSNSVDPQRIAMEVAILS
                     DRADITEECVRFRSHLDQFVNLMREDASGRKLNFLLQEMGREVNTTGSKSNDSTIAHL
                     VVEMKEEIERLREQVQNIE"
     gene            4537..5769
                     /locus_tag="P5179_09660"
     CDS             4537..5769
                     /locus_tag="P5179_09660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS95525.1"
                     /translation="MSELALLGGTPVRTKPFPAWPIWDEKDVEAITRALQSGKWGIAP
                     PDQSEVARFEKEYAAYHGAKHGICVHSGTTAIRIALQALGAGYGDEIIAPAYTFIGTV
                     SPIIDVGAVPVFVDIDPTTYTIDPAAVEAAITPRTVGIVPVHFGGCPCDMDALLAIAK
                     KHGLWIMEDAAQAWGAQWNGTGVGHIGAAGIFSFQSSKNVNAGEGGIVLTDDDDLSEL
                     IRSFSNCGRSSQGIWYGHYRIAGNYRMSQILAALLSVQFSRYPEQHRTRETNAAYLAR
                     KLAEIPGFTVLERKPQVTAHAQHLFIWRYDREAFGGLPRAKFIDAMHKEGIPISPGYS
                     IPLYKQPVFLEKNFDPRHAAQRVDFGSIQCVATEKACAEEALWLTQPILLGETADMDD
                     IVAAALKVRDNAHLLLER"
     gene            5795..>6015
                     /locus_tag="P5179_09665"
     CDS             5795..>6015
                     /locus_tag="P5179_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxybutyrate depolymerase"
                     /protein_id="HRS95526.1"
                     /translation="MARARAAAGAPAIAAIPGSAPFDAPTVDGFRAAHFTASNGVEMP
                     YRLFVPDSARDGQSYPLILYLHGRAGLGT"
BASE COUNT         1205 a         1834 c         1684 g         1292 t
ORIGIN      
        1 gcatcgctcc gcgccgtgac ggtgcaggtg tttaccaccg tcacgtccga aggctcctcg
       61 cgggaaacca cctcccagcc cgcccgggag aaatgccgct cgagcacctc ggtttcgcag
      121 ttgttgagtt tgcaccctac cgtgaggaaa ttgactcgca tggacggccc gaacagaatt
      181 cacagaagcg aaaaaccccg cagaaaaggt aagcccgtgg ccgcgaatgg gcaatgaacg
      241 gaccgggaac cggctctcta tcgcgatcgc gattcgtgcg tgccgcgccc atatcaggca
      301 ggcacgcgca ccgtaatccg gatggtccgc acgttgtaat gtgcgtcatt ccaccagtct
      361 tcaccgggca tctcgtattg ctccagtccc tggcggcctt tgccgggcgt ccattcggga
      421 gggcttacgt gcacgtcaag ctgaccgtat cccatcaccg tcaccttgcg gcaatccgga
      481 atttcgaagt gcacgctggc cagccccgcg tgccgatttc cttcctcgaa ctccggattt
      541 gcgccccagc ggtacaggtg ctcaccccag atattcgtct gtttgaactt cacacccgaa
      601 cgcccgtact tccactcgag aggttcgccg aacaccagga tggttcccgg agggatgcac
      661 gggctcccga ttacggtcac tccaatgatc ggctccgcct cttcgatcgt gatcgcgccg
      721 aaaggtggga tgtccagctc cattccgtcc gggtgggcgc ctgttcccgt tccgaaacgg
      781 cccagttcgc agttgacatt gggataattg aacggggctt catactcata cccccgcgtt
      841 gaatacacaa cccgtgtctg cggtgccttt tcctccgcaa gacgcggtat ttccgtttcc
      901 ttcggtgtca gttgaccggc tttttcccgt tccggattcc tgacggttac gcgcacaggt
      961 gtctttttcg acggtttgag gaaatccccg ctcgtggtgc ctgcactcat ggtgcctgcc
     1021 ctccttccgg tcccgaacac cctcgcccct gcgtcagcat tatgagcgca ttcgctctag
     1081 acttcttacg tgttcgaacc tacattacga taggcgacga ggccaatggt ttgtcgttct
     1141 tgtgctctcg tttgaaccgt tcagcggagg cacaacacgc agggcagagc agaaatgaaa
     1201 acgcacgcgc gccccatcac accggtaagc tcgctccacg cgacgattcg ccctcccggc
     1261 tcgaaaagcc tcacaaatcg cgcgcttttg tgcgccgcgc ttgcgtacgg cgaatcagag
     1321 atttccaatg cgctcgtgtc agacgacagc agatacatga tcagcgcatt gcggaaactt
     1381 ggcgtggacc tcagcgtgag cgcggacgga acgatgattc gcgtgcgcgg gtgttcgggc
     1441 agattcccgg caaagaaagc ggacctgttc gtcgggaatg ccggaaccgc aatgaggttc
     1501 ctcacggcgg cactgaccct cggcacgggg gtgtataccg ttgacggcga cgagcggatg
     1561 agacaacgtc ccattctcga cctcgtctac ggcctgcgcg ccctcggtgc tgatgtgctg
     1621 gcctgcaacg acccattccc gcccgtgctc atacgcgccg agggactcga cggaggcagt
     1681 tgcgagatcg atggcacaac cagcagccag tttgtcagtg ccgttctgat ggcagcaccg
     1741 tacgccagac gcgacaccgt ggtaaccgtg aaaaacggcc ttgtttccgc gccgtacgtc
     1801 ggtatgacac gccatgtcat gcgagcgttc ggtgcggacg tcgaagaaac cgagcctcat
     1861 gtctaccacg ttgccgcgaa ttctcgctac ttcggtacca cgttcgccgt ggaaccggat
     1921 gcctcggccg ggtcgtattt ttttgccgcc gccgcaataa ccgggggtac catccgcgtc
     1981 acaggcatcg ggaaaaacag cgtacagggt gatcgcgcgt ttgttcgtct gctcgaggca
     2041 atgggatgtg ttgtccgcga gggggccgac tggaccgagg ttacaggaaa acccctgcac
     2101 ggaatatcag cggatatggc ggacatctca gataccgcca tgacccttgc cgtcgtggcg
     2161 ctctttgcgg attctcccac cagtattcgc ggaatcggca atgtcagagt gaaggaatcg
     2221 gaccgcatcg ccgccatcgc gacagaagca aggaaactcg gggcacaggt ggaggagttc
     2281 gaggatggtt tgcgcatcac tcccgcaccg ctacgcggtg cgatgataga aacgtacaac
     2341 gaccaccgca ttgcgatgag tttcgccatc gcaggattgc gtcagcccgg ggttgtgatc
     2401 agcaatccgg aatgcgtgaa caagacctat ccgggtttct tcgaggaact cacgaaactc
     2461 ggcggctgac cacgagcact gaccgcgaca aatcccgcct gcgcactgcc ggttgccagg
     2521 aatcaggccc gcgcaagaaa cctacgcaga gatggcctgt tgctgccttc cggcgtcttc
     2581 cctgaccacc ttgaccgttc cattggcgat ttcttccagg gcctgcacgg ttggtttcgg
     2641 gccgaacgtc tcttcgccga ggatctttgc acgctgaatg cgccaatcgt ttattttgcg
     2701 tgctcttttc gccgcagcaa tcacgatgct gtacactgaa tgaccatgtc tcgtaagttg
     2761 atctaccagc agcatttcac gtctccgtct ttcctcgttc acaaagaccg atttatcatt
     2821 tcaccgcatt actacatctt cggcaaccaa tcaccttcca gatccatgcg cgaaatccga
     2881 cgggattcca ctctgatcac ctccagaatt gcctgaaccg cgttctcaac ccggtcgttc
     2941 cacaccaggt aatcgtagta cttcgcggcg gcaagttcag aagggacacg cgccagacgt
     3001 ttggcaatcg cctcttccgt ttctgttttc cgcgcgcgaa gtcgctgttc gagcgccgcg
     3061 agagagggcg gcgccacgaa aatcgtgacg gcatcaggca tcgttcgccg aatctgagcc
     3121 gctccctgaa tgtcaatgtc gagaaccgcg atgtcaccgt gggccttcgc cttttcgatg
     3181 aaagcccggg gcgttccgta gaaattcccc gcgaattctg cccattctat cagttcgccg
     3241 tcacgctttt tgcgctcgaa ctcatccagc gacacgaaga aatagtcctt gccgtcaacc
     3301 tcgccggcgc gcggctttcg cgttgtggct gatacagact gcacgcaacg gctttccgcc
     3361 tccagaacgg cctttgttat cgttgatttc cccgtgcccg agggtgctga aatcaccaga
     3421 gcaaatccgc gtgactgcat gggacacccc cctgttcatt cgatgttctg tacctgttcc
     3481 cggaggcgtt cgatctcctc tttcatctcc accaccaggt gcgcaatcgt cgaatcattg
     3541 gacttggacc cggtagtgtt gacttcccgc cccatctcct gcagaagaaa attgagcttg
     3601 cgccccgagg cgtcttcccg catcaggttg acaaactggt cgaggtgcga ccggaagcgg
     3661 acgcactcct cggtgatgtc ggcgcggtca gagagaatcg cgacttccat cgcaatgcgt
     3721 tgcggatcta ccgaatttga gccgagaagc gcagatattc gctctgtcag tttacgtttc
     3781 gcttcctcta cacgttccgg tgcacgtgcc tcaaccagag agacgagttc gcggagcctg
     3841 tcgatgcgga aaaggaagtc ctttgcaagg gcttctcctt ctttgcaccg catggtctgc
     3901 aactcctcga tcgcggcatc cagcgcgcca tcaagaagag cagccgccgt ttcgctttca
     3961 agctgagtgt cgcggacggc gagaacgccg tccatacgaa cgagcgccgc gaggtcgacg
     4021 tcagcagaca tacccagatg atctcgtacc gtctccactg cctttttgta cgcttcggcc
     4081 agtggcaggt ttggaatcac ctcgcgcacg gtggaagtgc cgttgccggc ggagttgtcg
     4141 agagaaacgt tgactgacac tttgcctctt cccagccgtt cctgaacaac ttcgatcaca
     4201 tgttgttcga gggaggaaag ggcgtgaggg atcctgcacg agacttcgat gaacttgctg
     4261 tttacggagc gaatctccac ggtgacgcaa tacccgccca ccgaggactg ccctcgtccg
     4321 aaacccgtca tgctgcgtat cacaaaaccg ccaccgtgga atcacccttc gagtttttcg
     4381 caatagagaa agcattagaa tgtactgaat ttctctgact ttgtcgagtg ttcctgcttc
     4441 ccggccgtcc tgcccgttga ccggttccca caggatcggt atcctttgcc cgagtcttgt
     4501 tttgcatgat ccacaattca caatggggac aacgcgatgt cggaacttgc tcttctcggt
     4561 ggaacaccgg ttcgcaccaa gccgtttcct gcctggccaa tctgggatga gaaggacgtc
     4621 gaggcgatca cccgggcgct ccaaagcggg aaatggggta tcgcgccgcc cgaccagagc
     4681 gaagttgcgc gatttgaaaa agagtacgcg gcgtaccacg gtgcgaaaca cggaatctgt
     4741 gtgcacagcg gaactacggc aattcggatt gcccttcagg cactgggcgc tggttacggg
     4801 gacgagatca tcgcccctgc atataccttt atcggaacgg tcagcccgat catcgacgta
     4861 ggggcagtac ccgtgttcgt ggatatcgat ccgacgacgt acaccattga cccggctgcg
     4921 gtagaagccg cgatcacacc ccgcacggtc gggatcgtgc cggtccattt cggcgggtgc
     4981 ccgtgtgaca tggatgcgct cctcgcgatc gcaaaaaaac atgggctctg gatcatggaa
     5041 gacgccgcac aggcgtgggg cgcgcagtgg aatggcacgg gagtcggcca catcggtgct
     5101 gcgggtattt tcagcttcca atcgtcgaaa aatgtgaatg cgggagaggg cggaatcgtt
     5161 ctcacagacg atgacgattt gtctgaattg atacgatcgt tcagcaattg cggccgttcc
     5221 agccagggca tctggtacgg gcattaccgg atagcgggta attaccgcat gtcgcagata
     5281 ctcgcggctt tgctctccgt tcagttttcg cgttaccccg agcagcatcg cacacgcgaa
     5341 acgaacgccg cgtatcttgc aagaaagctc gcggaaatac ccggtttcac ggttctcgaa
     5401 cgcaagccgc aggtgacggc gcacgcgcag catctgttca tctggcgata tgaccgtgag
     5461 gcgttcggag gattgcctcg agcgaagttc attgacgcga tgcacaagga aggcattccc
     5521 atctcgcccg gttattccat tcccctttac aaacagcctg tattcctgga gaagaatttc
     5581 gacccgagac acgccgcgca gcgcgtggac ttcggatcaa tccagtgcgt tgccacggaa
     5641 aaggcctgcg ctgaggaggc gctctggctg acgcaaccca tcctcctcgg cgaaaccgcc
     5701 gatatggatg acattgtcgc ggcggctctg aaagtcaggg acaatgcgca tctgctgctg
     5761 gagcgttaga gctgccttgt caatggaggc accgatggcg cgtgcgcgtg ccgcggcggg
     5821 agcccccgca atcgctgcaa ttccggggtc ggcacccttt gacgcaccca cggttgatgg
     5881 tttccgtgcc gcgcacttca cggcatcgaa cggcgtggag atgccctatc gtctttttgt
     5941 tcccgattcg gcacgcgacg ggcaatcgta cccgctcatc ctctacctgc acggacgggc
     6001 cggactgggc accga
//
LOCUS       DAOTNG010000193         5962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11360_length_5962_cov_4.671407, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000193 DAOTNG010000000
VERSION     DAOTNG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11360_length_5962_cov_4.671407"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..133
                     /locus_tag="P5179_09670"
                     /pseudo
     CDS             1..133
                     /locus_tag="P5179_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
     gene            complement(157..393)
                     /locus_tag="P5179_09675"
                     /pseudo
     CDS             complement(157..393)
                     /locus_tag="P5179_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
     gene            complement(479..1084)
                     /locus_tag="P5179_09680"
     CDS             complement(479..1084)
                     /locus_tag="P5179_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95527.1"
                     /translation="MRESTRGWSEQQWEDYFARQDESFFLALRSRTSREQPTNDVQPS
                     PETAGTMDDLLATASADTLLEEEDALIRELYAGLGQMPAWRAVVEFCDRTMEFVAPIC
                     ENVIGSPLESVTRILCRECFLVADYVLAGHELGYDEETLCGNIALCLRARRSLDRCIQ
                     CLDRFGPEYRDACLQLRLRGKIAGVFLERRVSDLRARVWWR"
     gene            1282..1944
                     /gene="eda"
                     /locus_tag="P5179_09685"
     CDS             1282..1944
                     /gene="eda"
                     /locus_tag="P5179_09685"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRS95528.1"
                     /translation="MEHPLLRKMLQNGIVGVVRTPTPETAARAVEAVVSGGIKVVEIT
                     FTVPNATTVLETVAASTGDDVVLGAGTVTDSEKAKAAIRAGARFIVAPNTDPATIATA
                     HTLGALAIPGALTPTEVVMAMNAGADAVKVFPISVFGPSYLKALRAPLPDVVLMPTGG
                     VEPDNVFDYLAAGAQLLGAGGTLVESKHIATGNYEAIARKARSFVDAYNEAIARLGSN
                     RS"
     gene            1966..2961
                     /locus_tag="P5179_09690"
     CDS             1966..2961
                     /locus_tag="P5179_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRS95529.1"
                     /translation="MSEVVTFGEGMVRLSPPHFQRLEQTSTLDMAVGGAEFNVATGVT
                     RLGLTASWVSRLPQNALGRVLVNQARQLGVDTSHVVWSRDDRMGIYFLEFGSAPRPST
                     VLYDRKNSAMSRIRPGEVDWDSALAGAKLFETSGITPALSPSAAEATAEALRIARLKG
                     LKTVYDPNYRSTLWSIEDARKVQTPLMRDVSCFITGVSMEGETKEAVLGVTGSSPHDL
                     AIRLVETFGFEVVALTLRGTSGLMRNSLTAVAYSNGRSYETKSYDLEVVDRVGGGDAF
                     AAGFIAGYLEDDIQKALDYGTAFAALKHTVPGDINWTTLQETEDLIKGAGLRIRR"
     gene            2993..3790
                     /locus_tag="P5179_09695"
     CDS             2993..3790
                     /locus_tag="P5179_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95530.1"
                     /translation="MRLSIVTDELKTPLAEALSILDGWKVRNIELRGLDGGRIPDGDV
                     ENALSRVRAHGCTVTSLSPGVFKCACDEQEIEKHIERFQRTLELCDSFGCGQVIVFSI
                     QGQESRLAAPPALVVEALRETGRIAARHGVRVAIENEPGYTATGCRALANLINAVGME
                     NIGANWDPGNAFPFDPEIDDAVRVLAGRIFNVHVKDTSVQGDHRRFDSVGKGQINWKK
                     QINDLAASAYEGCLVIETHCEPGIEKTRESLDAVRRLLQDVTPRIGG"
     gene            3798..4601
                     /gene="map"
                     /locus_tag="P5179_09700"
     CDS             3798..4601
                     /gene="map"
                     /locus_tag="P5179_09700"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRS95531.1"
                     /translation="MAQIKTLEDLERMAPACQLASETLEYLKQAVRAGISTLELDALA
                     EKYIADRGAIASPKGYRGYPNSICTAVNDVVVHGIPTAKPLEEGDLITIDVTVYKNGF
                     HGDKAATVIVPGKKNPEGERLLRVTEAAMYLGIAEARAGARLGDIGAAIQEHVEAAGF
                     SVVRDFCGHGIGRMFHEDPQVLNYGQRNTGRKLRPGMVFTVEPMINAGTWEVRILKDG
                     WTVITQDGSLSAQFEHTIAVTASAPRVLTLFTEHERAMWASYQKPDRAG"
BASE COUNT         1251 a         1739 c         1769 g         1203 t
ORIGIN      
        1 caacccctgg gacaccgact acaccgccgg cgcgtcatcg tcgggttcgg gggcgttcgt
       61 cgccgccggg gtggtgccga tcgcacacgc gaacgacgga ggcgggtcga tccgaattcc
      121 ggccgcctgc aacctgtcta ccaaccccag cgccagcgga tcgtccggcg ccacgagcac
      181 caggtccggt ttttccttcc ctgcaagtgc aacctgaccg tcaatgtccg tcggtttgac
      241 gtcgaagcac tccgcaattg acgctatgcc ggcgtttccg ggcgcagcca taaggcggtt
      301 cacaccgccc tggcgcttca acagccacgc aagggcgtgc tcacggcctc cctgaccgac
      361 aatcaggacg tcctttttct tcgtttcaaa catgctcatc ctttcgagtg tactgtcctt
      421 ccggccgatg acccggcctg atagctgtga gccgttctgg cttccctgtg gagcggttct
      481 agcgccacca gactcgtgcc ctaaggtccg aaacccgccg ttccagaaaa acgcccgcga
      541 tcttccccct tagacggagc tggaggcatg cgtcacgata ctccggtccg aaccggtcga
      601 ggcactggat gcatctgtcg agggaacgtc ttgcgcgcag gcacaatgcg atgttgccgc
      661 acagggtttc ctcatcgtat cccagttcat ggccagcgag aacgtaatcg gctacaagaa
      721 aacactcccg gcagagaatc cgcgtgaccg attccagcgg acttccgatc acgttttcac
      781 aaatcggggc aacgaattcc atcgtgcgat cgcagaactc cacaactgca cgccaggcgg
      841 gcatctggcc caacccggcg tacagttctc gaatcagggc gtcctcctct tcgaggagtg
      901 tgtctgcaga agcggtagca aggagatcgt ccatcgtgcc tgcagtttcg ggggatggtt
      961 gaacatcatt cgttggctgt tcgcgggaag tccgcgatct gagtgccagg aagaacgatt
     1021 catcctgcct cgcaaagtag tcctcccact gctgttccga ccacccgcgc gtgctctccc
     1081 gcatatgttc cttaccgcct tctcagcctt ctgccgaacc cctcttcgca cggaggaatt
     1141 cgaatgccgg aatgaattga atacctaatc gcgctccagc ggtcaactcc tcgaccgcat
     1201 gcgcccctgg gaatggcgcg ttttgacgcg tactttttgt ttgcatgtgc cgcggcctga
     1261 tggcagaacg gaggtaccct gatggaacac ccgttgttac gcaaaatgct gcagaacgga
     1321 attgtcggtg tcgttcgaac gccgacgccg gagacggcgg caagggctgt ggaggcagtt
     1381 gtttcaggcg gcataaaagt tgttgagatc acatttactg tgccgaacgc gaccacagtt
     1441 ctggaaacgg tggccgcttc gacgggcgac gacgtggtgc tgggagccgg caccgtgacc
     1501 gattccgaga aagcgaaagc agcaatacgg gccggcgcgc gattcatcgt ggcaccgaac
     1561 acggatcccg caacaatcgc cacggcacac accctcggag cgcttgccat acccggcgct
     1621 cttacgccga cggaagtggt gatggcgatg aacgcgggtg ctgatgcggt caaggtgttc
     1681 ccgatttccg tgtttggtcc gtcgtacctg aaagcgcttc gtgccccgct gcccgatgtt
     1741 gtgcttatgc caaccggtgg cgtcgaaccg gacaatgttt ttgactacct cgcagccgga
     1801 gcacaactgc ttggcgccgg gggaacgctg gtcgagtcaa agcacattgc aacgggcaat
     1861 tacgaggcaa ttgcacgaaa agcgcggtct ttcgtggatg cgtacaatga agcgatagcc
     1921 agactcggct cgaaccgatc gtgaagcggg aaggaacggt attccatgag cgaggtggta
     1981 acattcggcg agggcatggt acggctttcg ccaccacatt tccagcgcct ggagcaaaca
     2041 tcgacgctcg acatggcagt gggcggagcg gaattcaatg tcgcgaccgg agtaacccgg
     2101 ctgggtctga cagcctcgtg ggtttccaga ttgcctcaga acgccctcgg gcgggtgctg
     2161 gtcaatcagg cccgacaact cggagttgat acctctcacg tggtgtggtc acgggacgac
     2221 cgcatgggaa tctatttcct cgaattcgga tcggcaccac gtccttccac cgtgctgtac
     2281 gacaggaaga attcggctat gagccggatc cggccgggcg aggtggattg ggacagcgcg
     2341 ctggcgggag caaagttgtt cgagacttcg ggcattacgc ccgcactcag cccgagcgcg
     2401 gcagaagcga ccgcagaagc gctcagaatc gcgaggctga agggcctcaa gaccgtgtac
     2461 gaccccaact atcgcagcac cctctggtcc atcgaggacg cgcggaaagt gcagacgccg
     2521 ctcatgcggg acgtctcctg tttcatcacg ggagtgtcga tggaggggga gacaaaagaa
     2581 gctgttctgg gagtcacggg ttcgtcgccc catgatctcg cgattcggct tgtggagacg
     2641 ttcggatttg aagtggttgc gctgacgttg cgtggaactt ccggactgat gcgcaactca
     2701 ctgactgccg tcgcgtactc aaacggacgg tcctatgaaa cgaaaagcta cgatctggaa
     2761 gtagtggacc gggttggcgg gggtgatgcc tttgccgcgg ggtttattgc gggctacctg
     2821 gaggatgaca tccagaaggc actggactat ggcaccgcct tcgccgcgtt gaaacacact
     2881 gtccccggcg atatcaactg gacgactctc caggagacgg aggacctcat caagggtgcg
     2941 gggttgagga ttcgccgtta accgattctc tcacgcacca ctgaaaggac gtatgagact
     3001 ttccattgta accgacgaac ttaaaacacc gttggcggag gcgctgtcta ttctggatgg
     3061 gtggaaggtc cggaatattg agttgcgggg cctcgacggc ggcaggatcc ccgacggtga
     3121 tgttgagaac gcgctttccc gcgtccgtgc gcacggttgt acggtgacga gcctttcacc
     3181 gggagtcttc aaatgcgcct gcgatgagca ggaaatcgag aagcacatag aaaggttcca
     3241 aagaacgttg gagctctgcg attctttcgg gtgcggacag gtgattgtgt tcagcattca
     3301 aggacaggag tcccgtttag ccgcacctcc ggcccttgtt gttgaggcgc tgcgcgagac
     3361 cggccgaatc gctgcccggc atggagtgcg tgtcgcaatc gaaaacgagc ccgggtatac
     3421 cgctacaggg tgccgcgctc tggcgaacct gatcaacgca gtgggcatgg aaaacatcgg
     3481 cgcgaactgg gacccgggta acgcattccc ctttgacccc gaaatcgacg acgccgtccg
     3541 tgtgctggca ggacgcatat tcaacgtgca cgtgaaggac acttctgtac aaggtgatca
     3601 ccgtcgcttt gacagcgtcg gaaagggaca gatcaactgg aagaagcaga tcaacgacct
     3661 ggcggcatca gcctacgagg gctgtctcgt gatcgagacg cactgtgaac cggggattga
     3721 gaaaacacgc gaaagtctgg atgcggttcg cagactcctt caggacgtca caccccggat
     3781 tggaggctga actggccatg gcgcagatca aaacgctcga agatctggag cggatggctc
     3841 ccgcgtgtca gttggcttcc gaaacccttg agtatctgaa gcaggcagtt cgcgccggta
     3901 tttccacgct ggaactcgac gcgctggcgg aaaaatacat tgccgatcgc ggtgcgattg
     3961 caagtccgaa agggtaccgt gggtatccca acagcatctg cactgccgtc aatgatgtcg
     4021 tggtccatgg catcccgaca gcgaaaccgc tggaggaggg tgacctgatc accattgatg
     4081 tgacggtcta caagaacggc ttccatgggg acaaggccgc taccgtcatt gttccgggga
     4141 aaaagaaccc tgaaggggag cgcctgttgc gcgtgacaga agccgcgatg tacctgggaa
     4201 ttgccgaagc gcgcgctggt gcacgtctcg gcgacatcgg cgcggcgatc caggagcatg
     4261 tggaagccgc ggggttcagc gtcgtgcgag acttctgcgg ccacggaata ggaagaatgt
     4321 tccacgagga cccgcaggtg ctcaactacg ggcaacgcaa cacgggacgc aagcttcggc
     4381 cgggaatggt attcacggtg gagcccatga taaatgcggg aacatgggaa gtgcgcatcc
     4441 tgaaggacgg gtggacagtg atcacccagg acggcagtct ctcggcgcag tttgaacaca
     4501 caatcgccgt caccgcatcg gcccccagag tcctgactct cttcacggag cacgaacgcg
     4561 caatgtgggc ctcgtaccag aagccggacc gcgccggtta gcgcaacacg cagaacacct
     4621 ccctcatacc ccctcctcga aatacggctg cacttcgacg aaattgcaca ggagagtctc
     4681 gcccaatggc ccggctggcc tgccatcgga ggacgagccc tcccaatcgg aaatgaaaag
     4741 ctcggccgag ggtgaagttg cgcgaaacac gacctgatgg taattgaacc agtagcggtt
     4801 ctgcccttcg aatgcgccga gttcgtgtga gtaattttcc cgactacgct ctggaacgtt
     4861 tttttcggaa tcgtttcaac tcctgcgatc gaaagagcga tacccagttt ctccggcttt
     4921 gatttccccg caagaagctg atcatagctc cccgaaatca tcttcgcgct gtacattcgg
     4981 ccgggttgca ggttcttgat acgctgactt acgacattcg ggattttcgc gtcgcgtttc
     5041 atggtcaaga agctgtcccc aacggaggtt cgaggccagc gcccctgcag gtggccaaag
     5101 ccttcgcagt gccgcacgtg gacgttttcc tcgatatcca ctggcacgtg ccagccttcc
     5161 catccgaacg caaaatcggg attgcggaga tgacccggca cataggagta ccccagacgg
     5221 tctgacaaca ggtcggtttc cccttcgatc ccgtagtgcc ggtagagccg ggctgcccag
     5281 cgaagggttt cttcgtccgc gtatccgctg gtgtattcga ggatgccgaa aagccctcca
     5341 aaatccggat gattggccag aaatcggaac tgcatatcca taaagacctt gtagtcagcc
     5401 cctggatgga tgttcagcgt ctccgttatc gtcatgtatc cgaggcacac gagcagcgaa
     5461 cgggcacaat cggggaaccg gtgtcgccag tgggcgatcg tgcttcccag acgatcctgt
     5521 atgtgttcct ccgcgcaatc ctccgaatgg cgctcctgaa ggtaacgttc ccaggcaatg
     5581 ggataaccag cctgcatcgt cgtcgcgagg aaccggtaac ttgagtccac tccgtccgac
     5641 gcattgcgct tccagtcatc catgtccgga taatgcgatc cgaggtacgg gtagaccttc
     5701 ttaccctcga accgtttgtc ggaagctatt cgggacatcg cttccgtcca cgccttgtat
     5761 tggggccgcc gcccggagaa gaactcgtcc accatgatcc cgtccatcaa cgggtgaacc
     5821 attccggggc tggatgtcca gttccgccat acgtcatctg ctgtaacggt ttcgtcgttc
     5881 atcccgtgca ggctggtttc tacgatccat tttccgcccc tggctttcca ccacttcaga
     5941 tgctcttcat ggcttgggtc cc
//
LOCUS       DAOTNG010000194         5899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11501_length_5899_cov_5.086413, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000194 DAOTNG010000000
VERSION     DAOTNG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11501_length_5899_cov_5.086413"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5179_09705"
     CDS             <1..280
                     /locus_tag="P5179_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95532.1"
                     /translation="PVIVVTDAVLVARQTDGVLLVVRCNSTPREAARHTADTLRTANI
                     PLIGAVLNDIDVTRRYGGYHYYNYYYHYYYGGYYGSSDGTGDTKTAKT"
     gene            538..2325
                     /locus_tag="P5179_09710"
     CDS             538..2325
                     /locus_tag="P5179_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HRS95533.1"
                     /translation="MFQPVRAIAIFAAVSLLAAGASARPNTIGIGEIHEGMRGYGLTV
                     FSGVRIDTFQVEVLAVLRNQSGPADAIVARVSGGPLSETGVAQGMSGSPVYLDGRLAG
                     AVAWTSTFSKSPIAGITPIESMLEIPSRPMTPPPPDRYAFSERGAPQSSRSTIPRLAD
                     AGALAGTEMMPIKTPVAVAGADARALAFLQNILEPYGMTVLQSAGGTIGSDSLRNARL
                     EPGASLGVSLVRGDINVAGIGTVTWADSTTVIGFGHPMFSKGHVDLPMSLAYVHFLWP
                     SQVVSYKVASQGPVVGALRQDRGSGIAGVLNERPDMLPVEIKISGGPRPRVVHYEVVR
                     DIDLSPQLVTFGVVASLSELEALGIPASVEMTQIIQIEGRAPVRRKNFFTDFGGLMLA
                     AQAGAEPLDALARNPFAPVRIERVTYEISFRENIEAAFIKGLEIPRRVIRPGEPLIVR
                     AIFQTYLGRQFEKTLSLPISPETPDGVYILRVGGADAAQRWTVARSPGRFIPDNLDHL
                     IRLLNYEERNDNLTLEVVNRDIGMTVDDQELPSLPPSVFELLRSAVPGGRIGPILGTP
                     LVRKQEPMGLYVLGSQEISVAVYRFARPR"
     gene            2342..4411
                     /locus_tag="P5179_09715"
     CDS             2342..4411
                     /locus_tag="P5179_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95534.1"
                     /translation="MVRKLLFVLVLAASVMPVHRSGAVTPQRWFLSSAQDMESGDVEG
                     LSVADDGTLSLAPVLEDVGETEEMFVWALAEDRQGRLFAATGNQGRVFIKEGTQTPRL
                     FFETPQTQIQSLVIDPQGNVYAGAAPDGVIYKILPDGRGNVFCRTGETYVWDMELGAD
                     GSLYAATGTRGIVLKIDRDGKIVDKVLDTSDPHVMTLVPDGRGGFYAGTDGNGLVYAI
                     ENDRSRLLFAAAEREIHTLAVGPDGRVYAGATGARNPGEQSTERSPSGAVYRLEPSGA
                     ATKLWTAPYPLVLSIVPYDSSRMLVGVGPRGVLYLLSTDGRTQRVADTGESQPACFLK
                     RSSGEILIGMGNSGKIKRLTTKPGTRGVFVTPARNAGYVAQWGRVEIRGVIPPGTRVH
                     VESRTGNKSDPATEWSAWQPLFGEEQDRIQSPAAQYAQLRLTLTRAENAPSPQVSSIG
                     ITSLQVNLRPEISVVSVQPVRSTQTGSQSSSQQGESSSDQSSTPRTTTPRGAPTKRSL
                     VSVRWNVTDPNGDDLVYSLYFRRVSETTWHLLDELLTRNSYVWDTEGAPEGMTVVKVV
                     ASDQPSNPWQTALSAERESAAFPVDYSGPVVSELTASPRPDGSVVLRGVGSDTVGPIN
                     DGAYSVNSGEWRPFFPADGIFDGKREAFEVLTEALDPGTYAIAVRLRDSAENVGVGTV
                     TVQIPRR"
     gene            4424..5203
                     /locus_tag="P5179_09720"
     CDS             4424..5203
                     /locus_tag="P5179_09720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS95535.1"
                     /translation="MTDEGNAPGDRNDPLPWYAEWFGRDYLAVYAHRDEREAARQIDL
                     MERTLAPAPGARVLDLCCGGGRHSVELARRGYRVVGADLSPDLLETAQSAASASDLEV
                     VFVRADMRTCVGNGCFDVVVNFFTSFGYFETDEENARVLDAIRGNLRTGGGWMMDYMN
                     RDYVISRFEPRDERTTGEMRVVQQRRLDLLRGRILKTITIERPGRNDTYQESVRMYTA
                     PELMAMLAKTGLRVTHVFGDSDGAPYALDSPRLILAGTTAG"
     gene            5200..>5899
                     /gene="bshC"
                     /locus_tag="P5179_09725"
     CDS             5200..>5899
                     /gene="bshC"
                     /locus_tag="P5179_09725"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRS95536.1"
                     /translation="MRAQRLGNLQTVGLRGLAGKAISDGAFARRVYGGSAYEEADLRR
                     VAAAVRGRDYPRSELVSALRTQNESWGCTPETRKALRRLEDGALCVITGQQAGILTGP
                     LYTVYKALSAVAWARALESLLGEAVVPVFWIAGDDHDYEEVAHVAVPGRTGLPHHLEL
                     PRSLVSGAPSARVAELRFVGEIAGWADQTVSLLPDGPARDQVVCGIREAFAPGATFVD
                     AFARLMVTWLGRYGL"
BASE COUNT         1109 a         1705 c         1926 g         1159 t
ORIGIN      
        1 gcccgttatc gtggtgaccg acgccgtcct ggtggcccga cagacggacg gcgttcttct
       61 ggtggtccgt tgcaactcca cgccgcgcga agcggcgcgt cacaccgcag acacgctgag
      121 gacggccaat atcccgctga taggtgccgt gctgaacgat atagacgtga cgcgtcggta
      181 cggcggctac cactactaca actactatta ccactactat tacggcggct actacggaag
      241 ctctgacggc accggagaca caaaaacggc gaagacctga aacctgcgcc cgggtcgcga
      301 cggggctcgt gtgacgaatc cgagttttcc ccgggtgttg tggacttccg caaccgttgt
      361 ttcataatgc gcattttcag gcgtttcctg acgacaacag gcgttaattc acccccgggc
      421 gccttcctcc ttatattctg gatacaatcc cgcctgatct gacactgaga cgacactcgc
      481 gtgtttttcg gcagggttga ttccttgcgc atttgtctca aaagggtacc gacaagaatg
      541 tttcaaccgg tgcgagcgat cgcgattttc gctgcagtga gtcttctcgc ggcgggtgct
      601 tcggcgcgcc ccaacaccat cggaattgga gaaatccatg aggggatgcg cgggtacgga
      661 ctcacggtgt tttccggagt acggatagac acctttcagg tggaggttct cgccgttctg
      721 cggaaccagt caggccctgc ggacgctatc gttgcgcgag tgagtggagg tccactctcc
      781 gaaaccggtg tcgcccaggg gatgagcggg tcaccggtgt accttgatgg gcggcttgcg
      841 ggtgccgtcg cgtggacaag caccttttca aaatcgccga tcgccggaat taccccgatc
      901 gagagcatgc tggaaatccc cagccggccc atgactcctc ctcctccgga tcggtatgcg
      961 ttttcggaac gcggtgcacc acagtcgtcc cggagtacca ttccccggct ggcggatgcg
     1021 ggcgcccttg cggggacgga aatgatgccg atcaaaactc ccgtggccgt tgcgggcgcg
     1081 gacgcacggg ctctcgcgtt cctgcagaac attctcgaac cctatggtat gacggtgctg
     1141 cagagcgccg gcggcacgat tggatcagac tcgctgagga acgcaaggct ggaaccgggc
     1201 gcgtcactgg gtgtcagcct cgtgcgcggc gacataaacg tagccgggat cggaaccgtt
     1261 acgtgggcgg acagcaccac cgttatcggt ttcgggcacc cgatgttttc caaaggtcac
     1321 gtcgatctcc ccatgtcact tgcgtacgtg catttcctgt ggccaagcca ggtggtttcc
     1381 tacaaggtgg cctcgcaagg gcctgtggta ggggcgctgc ggcaggatcg gggaagcggc
     1441 atcgccgggg ttctgaacga acgaccggac atgctgccgg tggagatcaa gatttcggga
     1501 ggcccccggc cacgcgtcgt tcactatgaa gtggtgcggg acattgatct ttcgccgcaa
     1561 ctcgtgacgt tcggtgtcgt ggcaagcctt tctgaactgg aggcgctggg tattcccgca
     1621 tcagtcgaga tgacccagat catccagatc gaaggccggg cacccgtgcg gcggaagaac
     1681 ttcttcacgg atttcggggg gctcatgctg gccgcgcagg ccggagccga accactcgac
     1741 gcgcttgcga ggaatccgtt tgcgccggtg cgtatcgaac gggtgacgta tgaaatttct
     1801 ttccgtgaga acatagaggc agcttttatc aaagggctgg agattccccg tcgggtgatc
     1861 cggccgggtg agccgctcat cgtccgggcg atttttcaga cctatctcgg aaggcagttt
     1921 gagaaaaccc tttccctccc gatttctccg gaaacccctg acggcgtgta cattctccgg
     1981 gtgggtggcg ccgacgcggc ccaacgctgg accgttgcga ggtcgccggg acgtttcatc
     2041 cccgacaatc tcgaccacct gattcgcctt ctgaattacg aagagcgtaa tgataatctt
     2101 actctcgaag tggtgaaccg ggatatcgga atgacggtgg acgaccagga attgccgagc
     2161 cttccgccca gcgtgttcga actcctgcgc agtgcggttc ccgggggaag aatcggccct
     2221 attctcggaa cgccgctcgt tcgcaagcag gagcccatgg ggctgtacgt tctgggttct
     2281 caggaaatca gcgtcgcggt ataccggttc gcgcggccac gctagtccga cggagaagag
     2341 aatggttcgg aagcttctgt tcgtattggt tcttgccgcg tctgtgatgc ctgttcacag
     2401 aagcggcgcc gttactcccc agaggtggtt cctttcctcg gcgcaggaca tggaaagcgg
     2461 cgacgtggaa ggcctttccg tggccgatga tgggacactg agccttgcgc cggtgttgga
     2521 ggacgtggga gaaaccgagg agatgttcgt gtgggctctt gcggaggatc ggcagggccg
     2581 gcttttcgcc gcaacgggaa atcagggacg cgttttcatc aaggagggaa cccagacacc
     2641 ccgcctgttt ttcgaaactc cccagacgca aatccaaagc cttgtgattg acccgcaggg
     2701 caacgtctat gcgggagcgg cgccggacgg cgtgatctac aaaatcctcc cggatggccg
     2761 gggcaatgtg ttctgccgca ctggagaaac gtatgtctgg gatatggaat tgggcgcgga
     2821 cgggtcactg tacgcggcca ccggcacacg cggcatcgtg ctgaagatag accgtgacgg
     2881 caaaatcgtg gacaaggtgc ttgacacgag cgatcctcac gtgatgacgc tggttcccga
     2941 cggacggggc ggtttctacg cgggcaccga cgggaatggt ctcgtgtacg ccatcgaaaa
     3001 cgatcgatcc cgccttctgt tcgcggcggc ggagcgggag atccacacgc tggccgtcgg
     3061 gcctgacggc cgggtgtacg cgggcgcgac gggagcgaga aaccccggcg aacagagcac
     3121 ggaacggtcg ccctccggcg cggtctaccg tctggagcct tccggagcgg cgacgaagct
     3181 ctggacggcg ccgtaccctc ttgtgctctc cattgtgccg tacgactcct cgcgcatgct
     3241 cgtcggtgtt ggcccgcgcg gggtgctcta ccttctgagc accgatggcc gaacgcagcg
     3301 ggtggctgac accggcgaat cccagcctgc gtgtttcctg aagcgttcgt cgggcgaaat
     3361 cctcatcggc atgggcaatt ccgggaaaat caaacgcctg acgacgaaac ccgggactcg
     3421 tggcgtgttt gtcacgcccg cgagaaacgc cgggtacgtc gcgcagtggg gacgcgtgga
     3481 gatacgggga gtcatccccc ccggcacgag agtgcatgtc gaatcgcgca ccgggaacaa
     3541 gtcggacccg gcgacggaat ggagcgcctg gcagccgctt ttcggcgagg aacaggaccg
     3601 gatacagagc ccggcggcac agtacgcgca actccggttg acgcttacaa gggcagaaaa
     3661 cgcaccctct cctcaggttt ccagcatcgg gattaccagc ctgcaggtga acctgcgtcc
     3721 cgagatttca gtggtgtcgg tccagccggt tcgatccaca cagacaggtt cgcagagctc
     3781 ctcgcaacag ggtgaaagct caagcgatca gtcgtccacc ccgcggacaa ctactccgcg
     3841 cggggcgccg acgaagcgat ccctcgtttc ggtacgctgg aatgtcaccg atcccaacgg
     3901 cgatgatctg gtgtactccc tctatttccg acgggtgagc gaaacgacat ggcatctgct
     3961 ggacgaattg ctcacgcgga attcctacgt atgggatact gaaggcgcgc ccgagggtat
     4021 gaccgtggtg aaagtagtcg cgtcggacca gccgagtaat ccgtggcaga cggctctttc
     4081 cgcagagcgg gaaagcgctg cgtttccggt cgactactcg ggtcccgtgg taagcgaact
     4141 gactgcctct cccaggccgg acgggtccgt cgtgttgagg ggtgtggggt cagataccgt
     4201 cgggccgatc aacgacggtg cgtattcggt gaacagcggg gaatggcgtc catttttccc
     4261 tgcggacggc atattcgacg ggaaacggga agcgtttgag gtgctgaccg aggcgcttga
     4321 ccccggaacc tatgcgatag ccgtcagatt gagggacagc gccgaaaacg tgggagtggg
     4381 tacggttacc gtccagattc cgcggcgtta agagggggca tccgtgaccg acgaggggaa
     4441 cgcacccggg gacaggaacg accccctgcc ctggtatgcg gaatggttcg ggcgggatta
     4501 cctcgccgta tatgcgcacc gcgatgagcg cgaagcagcg cgtcagattg acctgatgga
     4561 acgcaccctc gcccctgccc ccggtgcccg agtgctcgat ctctgctgcg ggggcggacg
     4621 tcattcagtc gaactggcac ggcgtgggta ccgcgttgtc ggtgctgatc tttctccaga
     4681 cctccttgaa acggcacaaa gcgctgcgtc cgcgagcgat ctggaggtcg tgtttgtgcg
     4741 cgccgacatg cggacgtgcg tagggaacgg gtgcttcgat gtcgtcgtga actttttcac
     4801 gagcttcggg tatttcgaaa ccgacgagga aaacgcccgt gtgcttgacg ccattcgggg
     4861 aaatcttcga acgggcggcg gatggatgat ggactatatg aatcgcgatt acgtgatctc
     4921 gcggttcgaa cctcgtgacg agcggacaac cggcgagatg cgtgtggtcc agcaacggcg
     4981 tcttgatctc ttgcggggga gaattttgaa gacaattacc attgagcggc ccgggcggaa
     5041 cgacacgtat caggagtccg tgaggatgta cactgccccg gaactgatgg caatgcttgc
     5101 gaaaacaggg ctgcgcgtca ctcacgtgtt cggcgactcc gacggcgcgc cgtacgcgct
     5161 cgattctccg cggctcatac tcgcaggaac gactgccgga tgagggcaca acgcctcggc
     5221 aatcttcaga cggtaggtct tcgtggactg gcggggaagg ccatctcgga cggagcgttt
     5281 gcccgtcgtg tgtacggggg cagtgcctac gaagaggctg atcttcggcg ggttgctgcc
     5341 gcggttcgcg ggagggacta tccgcgctcc gaactcgtat ccgccctccg aacccagaat
     5401 gaatcatggg gctgtacgcc ggaaacgcgg aaggcattgc gcaggcttga ggacggagcg
     5461 ctctgcgtaa tcacgggtca gcaggcggga attctcaccg ggccgctata caccgtgtac
     5521 aaggcgctct ccgcagttgc gtgggcccga gcgttggagt ctctgcttgg cgaggcggtc
     5581 gttcccgtgt tctggatcgc cggggacgac cacgattacg aagaggtcgc acacgtagcg
     5641 gttcccggac ggacaggcct tccgcaccat ctggaacttc cccgttcgct ggtttcgggg
     5701 gcgccctcag cccgcgtagc ggagttgcgt ttcgtcggcg agattgcggg atgggcggac
     5761 caaacggtct ccttgcttcc cgacgggccg gcgcgggacc aagtggtctg cggaattcgg
     5821 gaagccttcg caccgggtgc cacctttgtg gatgcgtttg cgcgcctcat ggttacgtgg
     5881 ctgggtcgct acgggctca
//
LOCUS       DAOTNG010000195         5887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11529_length_5887_cov_5.379115, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000195 DAOTNG010000000
VERSION     DAOTNG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11529_length_5887_cov_5.379115"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="P5179_09730"
     CDS             complement(<1..337)
                     /locus_tag="P5179_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95537.1"
                     /translation="MAGLRDKLWLWGTGVNCLAKDYGFPESRMTIGGGLRELGIDQAM
                     MCGFLPPTEEEYRDVAFCRNLLWEMSFDDGFQFERPLAPIIALHEAHPNVRGVLLDDF
                     STTEISKGAQ"
     gene            complement(349..1950)
                     /locus_tag="P5179_09735"
     CDS             complement(349..1950)
                     /locus_tag="P5179_09735"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRS95538.1"
                     /translation="MERLQIGDSAGWHFENGAWVDTDGGGIGIPQEIRRQSGDGLQGH
                     HYAFHLGADYGDFRARLRFSLTPHSDAGLIFHAVAPDDFFVLHFPECGQADRAQHFWA
                     AVSRMSGEGWLRIVRMELVRRVSSTTVAWHEADIQLAGNRLFVRIDGRGVFDVTDPSF
                     VGYGRVGLFLFNNASIVDASVGGAKKSARAWPADKSPRTTWFHPVNGMEPGTWQRPGQ
                     IVRAGDDLLLSFTTQKPAYSGTTAEFVTRSTDNGRTWSPPERVTTHEAEAWDGWGYLH
                     RWPDGVLRMLISSETGARMAEGGPDGKLWRAPKPIPMPAAPSGLKRVYPAPLLNLSDG
                     SVLAFGYGGHDSTIPGSSIFTWGSCHCQAFAARTTDNGATWSNWVNIDGTRDSTGKPV
                     DGSLDLTEICGAEVAPGRIVALIRPVYSPWMWETWSEDGGRTWTPCVRGPFPGYATSN
                     MLRTTSGVLLVAHRLPGCTIHASHDDGVSWDTGTQIDSAIWVMGSMIEVEPDRVLYVY
                     WDSFESSMRAQFIRVTPRGIVPEAR"
     gene            complement(2162..2833)
                     /locus_tag="P5179_09740"
     CDS             complement(2162..2833)
                     /locus_tag="P5179_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HRS95539.1"
                     /translation="MSERELLIPVHGGYRHLKSFRVAQLVYDLTMRFCDSYVGKRSRT
                     HDQMVQAARSGVQNIAEGSQASGTSKKMELKLTNVARASLEELRLDYEDFLRQHGLPR
                     WERTDPRRQSLVDSRPATADDVARWAKRLHGEAEQGGRSGPGGLPKEIGASTTSITST
                     TRAVQKPPPTYPEIAANGALVLIAVAASLLDKQLAAQAGSFEKEGGFTERLHRVRSQN
                     RPNPA"
     gene            complement(2867..4372)
                     /locus_tag="P5179_09745"
     CDS             complement(2867..4372)
                     /locus_tag="P5179_09745"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRS95540.1"
                     /translation="MVSLGEVLRSTAAKTPEKTAVVFQSRKTTYRALDRMTDGLAAHL
                     RNAGIEPGDRVALICINCTAFVVAYYGILKAGAVVVPINVLLNADEVRHIIADSGSRG
                     VIYHELLAPMVDGLGTALEEMRVKIRLSNDDEANAELFAPPKRAWKIPPINPVEDLAA
                     VMYTSGTTGRAKGAMMTHANLISNAAAYTVHMGVTSRDVTVVVLPMFHTFAATVGTNA
                     SIYIGATITVLVRFTPDEVARVMEEDGATVFMGVPSMYTIFCNLPEGKAPPLERLRVA
                     VSGGAALPVDIHRRFEERFGVGIREGYGLTECSPITTMTPITGVIKPGSIGPALEGVE
                     VRLVDDRGNDVPAGEVGELVVRGPNVMKGYWENEKATREAFFGEWFRTGDLARVDEDG
                     YYYIVDRKKDLIIVNGLNVYPRHVEEVIYQHPAVAEVAVVPEKDDLHGEVPRAVIALK
                     EGASATEREIIAFCRKRLGRFQVPRVVEFVPALPKNATGKILKRAIPPRVR"
     gene            4509..4976
                     /locus_tag="P5179_09750"
     CDS             4509..4976
                     /locus_tag="P5179_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRS95541.1"
                     /translation="MKLPPWAMLVLFLIVVFSVSVIGGLFTASSVGTWYEGLAKPSWR
                     PPNWLFGPVWTLLYAMMAVSAWLVWKKAGFGPALLMFAVQLLINGIWSPVFFGAQSLG
                     GGLVVIVALWFAIVGTIAMFWPVSLAAALLLLPYLAWVSFATVLNAAIWRMNP"
     gene            4987..5880
                     /locus_tag="P5179_09755"
     CDS             4987..5880
                     /locus_tag="P5179_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRS95542.1"
                     /translation="MRVLLSGSTGLIGGELLWRLKAEGHHVTRLVRSPAPFPEPVVVW
                     DYGMGPFDPAELSPIDAVIHLAGENISTGRWTAARKERIRSSRIDSTRLLAEAIARAA
                     QPPAVFIVASAVGYYGDRGNELVTEDSAPGTDFLAQVVLDWEAAAKPAAEAGVRVVHT
                     RFGVVLSSRGGALAKMLPLFRAGVGGRLGSGNQFMSWVALEDATAAIAHALTTDTLRG
                     PVNVVAPKPVTNREFTQALGAALGRPTFFVVPRVALRLVYGEMADATLLSSTRASAEK
                     LVSSGFSFRHPTLETVLNVLG"
BASE COUNT          963 a         1991 c         1829 g         1104 t
ORIGIN      
        1 gttgcgcgcc cttgctgatt tccgtggtgc tgaagtcgtc cagcaacacc ccgcggacgt
       61 tgggatgcgc ctcgtggagc gcgataatgg gcgccagcgg ccgctcaaac tggaacccgt
      121 cgtcgaagct catctcccag agcagattcc ggcagaacgc aacatcgcgg tattcttcct
      181 ccgtcggcgg gaggaagccg cacatcatcg cctgatcaat gccaagctcg cgcagcccgc
      241 cgccgatggt catgcgggat tccgggaagc cgtaatcctt cgcgaggcaa ttcacgcccg
      301 taccccagag ccagagtttg tcgcgtaatc ctgccatggt gcctctcctc aacgagcttc
      361 ggggacgatg ccccgcgggg tgacgcggat gaactgcgcc cgcatggacg actcaaacga
      421 atcccagtag acatacaaca cgcggtcggg ctccacctcg atcatactgc ccatcaccca
      481 gatggccgag tcaatctgcg tgccggtatc ccacgacacg ccgtcatcat ggctcgcgtg
      541 aatcgtgcag ccgggcaggc ggtgcgccac cagcagcaca cccgacgtcg tgcgcagcat
      601 gttcgatgtc gcgtagccgg ggaacggccc gcgcacgcac ggtgtccacg tccggccgcc
      661 gtcctccgac cacgtctccc acatccacgg cgaatagacc ggccggatca gcgccacgat
      721 gcgaccgggt gcaacctccg cgccgcagat ctccgtcagg tcgaggctgc cgtccaccgg
      781 tttgccggtg gaatcgcgcg tgccgtcaat gttcacccag ttcgaccagg tggcgccgtt
      841 gtccgtcgtc cgcgccgcaa acgcctgaca gtggcacgag ccccacgtga aaatgctcga
      901 gcccggaatc gtgctgtcgt gccctccata cccgaaggca agcacggagc cgtccgacag
      961 gttgagcagc ggagcggggt agacccgttt caacccgctc ggcgcggccg gcatggggat
     1021 cggcttcgga gcacgccaga gcttgccgtc tgggccgccc tcagccatgc gggcgcccgt
     1081 ttcgctggag atcaacatgc gcaggacgcc gtccggccag cggtgcaggt agccccagcc
     1141 gtcccacgcc tccgcttcgt gcgtcgtcac gcgctccggt ggcgaccagg tccggccgtt
     1201 atccgttgac cgggtgacaa actcggccgt cgtgccgctg taggcaggtt tctgcgtggt
     1261 gaagctgagc agcaggtcat ctcccgcacg gacaatctgc ccggggcgct gccaggtgcc
     1321 gggttccatg ccgttcaccg ggtggaacca tgtcgtgcgg ggggatttgt ccgccggcca
     1381 cgctctcgcg cttttctttg ccccgccgac ggatgcatcg acgatcgacg cgttgttgaa
     1441 gagaaaaagc cccaccctgc cgtacccgac gaacgacggg tcggtcacgt cgaacacgcc
     1501 ccgcccgtca atccgcacga acaggcggtt gccggcaagc tggatgtccg cttcgtgcca
     1561 tgccaccgtg gtgctggata cgcggcggac aagctccatg cgcacgatgc gcagccagcc
     1621 ctcgcccgac atgcgggata ctgccgccca gaagtgctgc gcacggtcgg cctgcccgca
     1681 ctcgggaaag tgcagcacga agaagtcatc cggcgccacg gcgtggaaaa tcagcccggc
     1741 gtcgctgtgc ggcgtcaggg aaaaccgcag ccgggcccgg aaatcaccat agtccgcgcc
     1801 gaggtggaac gcgtagtggt gcccctgaag cccgtcgccc gattgcctgc ggatttcctg
     1861 ggggatgccg atgccgcccc cgtccgtatc cacccatgcg ccattctcaa agtgccagcc
     1921 cgcgctgtcc ccgatttgca accgctccat ccggtgtacc tcctgaagag cctgttccgc
     1981 gttcccggtg cgtgttcata atggcgaatc tgtacgcgcg gcccaactcc tcgcactgat
     2041 ttgccctgtg gcccggctcg tatcagctat ggcgaggagg cgtcttcgtg tcaagtgttt
     2101 cgtccgaaaa tgtttcaatc gcggtacggg tgtttcggag agaaccgttc gggcgggcag
     2161 ttcacgcagg gtttgggcgg ttctgagacc gcacccggtg cagtcgctcg gtgaagccgc
     2221 cctccttttc gaacgatccg gcctgcgcgg ccagttgctt gtccagaaga cttgccgcca
     2281 cggcgatcag gacgagtgcc ccgtttgcgg cgatttcggg gtaggttggc gggggcttct
     2341 gaacagcccg ggttgtggac gttatggacg ttgtggacgc cccgatctcc ttcggtagtc
     2401 caccaggtcc acttcgtcca ccctgttctg cttctccgtg cagcctcttt gcccaccgcg
     2461 ccacgtcgtc tgccgtggcg ggccggctgt ccaccaggct ctggcggcgc ggatcagttc
     2521 gttcccaccg gggcaggccg tgctgccgaa ggaagtcctc gtagtccagg cggagttcct
     2581 caaggctggc acgcgcaacg ttcgtcagct tcagctccat cttcttcgag gtaccggacg
     2641 cctggctgcc ctcagcgatg ttctgcacac cactgcgggc agcctgaacc atctgatcgt
     2701 gggtgcggct gcgttttccg acgtagctgt cgcagaacct catggtaagg tcgtacacaa
     2761 gctgcgcaac acggaagctc ttcaggtggc ggtacccccc gtgtactgga atcagcagtt
     2821 ccctctccga catcagcttg tcctccgcgt ccactccgtc cgccttttac cgcaccctcg
     2881 gcgggatggc cctcttcagg atcttccctg tggcgttctt cggcaacgcc ggcacgaact
     2941 ccacaacgcg gggcacctga aaccggccaa gacgtttccg gcagaacgcg atgatctccc
     3001 gctccgtggc gcttgccccc tccttcagcg cgattacggc gcgcggcact tcaccgtgca
     3061 ggtcatcctt ctccggcacc accgccacct ccgccaccgc cgggtgctgg tagatcacct
     3121 cctccacgtg ccggggatag acgttgagcc cgtttacgat gatgaggtcc tttttccggt
     3181 ccacgatgta gtaatacccg tcctcgtcca cccgtgcgag atcccccgtg cggaaccact
     3241 cgccaaaaaa cgcctcacgt gtggctttct cgttctccca gtaccccttc atcacgttcg
     3301 ggccgcgcac cacaagctcg cccacctcac cagccggaac gtcgttcccg cggtcatcca
     3361 ccaggcggac ctccacccct tcgagcgccg gaccgatgga acccggcttg atcacgccgg
     3421 tgatcggtgt catagtggtg atggggctgc attcagtgag tccgtaacct tcccggatgc
     3481 cgacgccgaa cctctcctca aaccgccggt gaatatccac gggcagcgcc gcgccgccgg
     3541 aaactgccac gcgcaggcgc tcgagcggcg gcgccttccc ctccggaagg ttgcagaaga
     3601 tcgtgtacat ggatggcacg cccatgaaga ctgtcgcgcc gtcctcctcc atcacccttg
     3661 cgacttcatc cggcgtgaac cgcaccagca ccgtaatggt ggcgccgatg tagatgctcg
     3721 cgttggtgcc gaccgttgcc gcaaatgtgt ggaacatcgg cagcacgacc acggtaacgt
     3781 cgcgcgacgt tacccccatg tgcacggtgt acgccgcggc gttcgaaatg aggttggcgt
     3841 gcgtcatcat cgcgcccttc gcgcggccgg tggtgccgga ggtatacatg accgcggcaa
     3901 ggtcttccac cgggttgatc ggcggtatct tccacgcgcg cttcggcgga gcgaacaact
     3961 cggcgttggc ctcatcgtcg ttgctcaggc gaattttgac acgcatttct tccagcgccg
     4021 tccccagccc gtccaccatc ggcgccagaa gctcgtggta gatgacgccc cgcgatcccg
     4081 agtcggcgat gatatggcgc acttcatcag cgttcagcag cacgttgatc ggcaccacca
     4141 ccgcgccggc cttgagaatc ccgtagtacg ccaccacaaa cgccgtgcag ttgatgcaga
     4201 tgagcgccac gcggtcgccc ggttcaatgc ccgcgttccg gaggtgcgcg gcgaggccgt
     4261 cagtcatgcg gtcgagcgca cggtaggtgg tcttccgcga ctgaaacacg acggccgttt
     4321 tttcgggcgt cttcgccgcg gtggagcgga ggacttctcc aagagacacc atcgttcgat
     4381 attccttgtg cgggtgttgg agcgcgcggc acgggtgccg cgtacacaaa gataggccgc
     4441 cggccggccg ggtcaaaaaa agcgccacaa accccgaccg caggcgtaca accggggagg
     4501 gagctcctgt gaaactgcca ccgtgggcca tgcttgttct gttcctcatc gtcgtgttct
     4561 cggtttccgt aattggcggc ctgtttaccg cgtcatctgt cggcacatgg tacgaagggc
     4621 tcgccaaacc ctcctggcgg ccgccgaatt ggctgttcgg ccccgtgtgg acgctactct
     4681 acgccatgat ggcggtttcc gcgtggctgg tatggaaaaa ggcaggattc ggcccggcgc
     4741 tgctgatgtt tgccgttcaa ctgctgatca acgggatctg gtcgccggtg ttcttcggcg
     4801 cgcagagtct cggcggcgga ctcgtcgtca ttgtagcgct ctggttcgcc atcgtcggca
     4861 ccatcgcgat gttctggccg gtcagcctgg cggcggcgct cctgcttctg ccgtaccttg
     4921 cctgggtgag tttcgccaca gtcctgaacg cggcaatctg gcggatgaat ccgtaaggag
     4981 gcagtcgtgc gtgttctgct gagcggttcc accgggttga tcgggggcga actgctgtgg
     5041 cggctgaaag cggaaggcca ccacgtcacc cgcctcgtgc ggtcgcccgc accgtttccc
     5101 gaacccgtgg ttgtgtggga ttacggcatg ggcccgttcg atccggcgga actgagcccg
     5161 attgacgcgg tcatccacct cgccggagaa aacatcagca cagggaggtg gactgccgca
     5221 cgcaaggagc gcatacggtc gagccgcatc gattccaccc gtctcctcgc ggaggctatc
     5281 gcgcgcgccg cgcagccgcc ggcggtgttc atcgtggcgt cggccgtcgg gtactacggc
     5341 gaccgcggca atgaacttgt caccgaggac tccgcgcccg gaaccgattt cctcgcgcag
     5401 gtggtgctgg actgggaggc cgccgcgaaa cccgccgcgg aggcgggcgt cagagtggta
     5461 cacacccggt tcggggtggt gttgagcagc cgcggcggcg cactggcgaa gatgcttccc
     5521 ctgttccgcg ccggcgtggg cggtcggctc ggttccggaa accagttcat gagctgggtg
     5581 gcactcgagg acgccacggc cgcgatcgcg cacgcgctga ccaccgacac actgcggggc
     5641 ccggtgaacg tcgtcgcacc gaagccggtc accaatcgcg agttcacaca agccctcggc
     5701 gcggcgctgg ggcgtccgac ctttttcgtc gttccgcgcg tcgcactccg cctcgtgtac
     5761 ggtgagatgg ctgacgcaac gctgctatcc agcacccgcg ccagtgcgga gaagctcgtg
     5821 tcttccggct tcagcttccg gcatccgacg ctggagacgg tgctgaacgt gctgggatga
     5881 ccgtcgg
//
LOCUS       DAOTNG010000196         5868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11576_length_5868_cov_4.445897, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000196 DAOTNG010000000
VERSION     DAOTNG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11576_length_5868_cov_4.445897"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5179_09760"
     CDS             <1..194
                     /locus_tag="P5179_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95543.1"
                     /translation="RIDGMIIPSRLPENKPSIMEAWISAILKDTPLITTVEDGRNLTE
                     MLEGCYTAWRTGKRYAFAK"
     gene            complement(231..833)
                     /locus_tag="P5179_09765"
     CDS             complement(231..833)
                     /locus_tag="P5179_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95544.1"
                     /translation="MRWTSWLPYAVIAAAVTAAAVNLPPSEMERSVAEGIVHSAAQSP
                     FWLFVGGALSDDFVSSAAIALTTLLLYVFLPTKESVIPLAALGGAAVLDRLLKGIMFS
                     STGASVPDVPSSAVLLASAWAGSVALVVARTGRMTRWRKAALTGAVLVVLLSGASALI
                     AKRSWPMEILAAWLWAGAVVFWLHRATRNVLTGKGIREGT"
     gene            complement(830..2116)
                     /locus_tag="P5179_09770"
     CDS             complement(830..2116)
                     /locus_tag="P5179_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS95545.1"
                     /translation="MSTSAASTGFPDSESVSRTVLPDGAILLVRENPASPAVVVQGAI
                     LTGAVDDPLDRPGLCRFTAECLTRGTQRRSAAELFELVESVGGSLGVGAGLHTVRFGA
                     KCLSDDLQMIVELLSEVLHSPSFPAAEIEKVRGEVLTELEERDNDTRSVTDLAFRKLA
                     YPQDHPYSRPADGERETVAGFTRDDVQHFFDSLVRRRPAIISVVGNLRQDDVEKLFAP
                     LWSTNARTANPAPLPDCTRRDGPRETYTVIPGKVQIDLAWGTIGPPRNHPDFVPASVA
                     NLILGGFGLMGRLGQKVREELGLAYYAASRVNAGLGAGPWVCVAGVAPEHYRKAVSAI
                     EQEVAKLRETPVPQDELEDCKSHLIGSLPLKLESNEGVASIMSEIEIYRLGWDYLHRF
                     ADLVNSVTAEDTNRVVRAYFEPDQAILAGSGPEVPG"
     gene            complement(2113..3444)
                     /locus_tag="P5179_09775"
     CDS             complement(2113..3444)
                     /locus_tag="P5179_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS95546.1"
                     /translation="MANPPYQVSVLTNGLTVVTEERHHAPVTSFWMGYRVGSRNELPG
                     QTGVSHWIEHMLFKPSQRFPGIERDRLIAREGGTTNAFTWIDCTAYYTTIPSSKLDIV
                     LQIEADRLSGPLFAPDVFETERSVILAERDAYENQPEWRLAEQVTAAAFHAHPYGHEV
                     IGIAEDIRNLSREDLLAYFRTYYIPNNAVCVVVGDFETRVLLGRLEELFSEIPPGATP
                     PAPRAIEPAPVAERRVLLEGPGATGYLEMAFLAPKATDPRFVPSLVLGSILGSPISLG
                     FGSGAESRSSRLYRALVEKELAIDVDCNLEASIDPFLFDITAVVRDGRTHGEVEGAIL
                     RELEAIAASPPDAAEMAKARKQLRALFAFTNERITHRAMVLTLGFTVFPVQFLDEFEQ
                     RISDVTAEDVRAAAESLFARRNRVVGWYQPLGGEDELEDADEETETEEGIQ"
     gene            complement(3463..3948)
                     /locus_tag="P5179_09780"
     CDS             complement(3463..3948)
                     /locus_tag="P5179_09780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95547.1"
                     /translation="MSEPVYEIAGVEAVDEAVKVHEAAFYEWDGAKDLQKGAASWRQT
                     LTEKPGALLTVARLDGRIVTYVLGHDAQEPGVFFIALMGGLPEIRGKGIGRGLFRFTL
                     EEIRRRGYRSVDINTLNRYRGMLTLCITEDFDIVDLRFDQGRACNRIYLRRVFGPHVR
                     P"
     gene            4113..4922
                     /locus_tag="P5179_09785"
     CDS             4113..4922
                     /locus_tag="P5179_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRS95548.1"
                     /translation="MDKGQEQLMCDNTDIMESVKEIAEEAGQIALSQFGASRPHVKTD
                     RSFVTATDREVEQFIHRKLHELFPDDAIIGEEFGGSPGTIREYVWLLDPIDGTTNFVA
                     GLPHWAVCVARLRFGVPVLGVVVLPVLRETYAAATGIGATLNGSPFKMLERDDPPNEQ
                     LIAVWSTWYREVDLKFSGKVRTLGSTVVKCLYAARGSFVGALTPEVHAWDIAAGLPVL
                     WESGGQAYARDGKVYEVMDLDPANGYLVPPLILSAPSRRDFMRQMITARRT"
     gene            4945..>5868
                     /locus_tag="P5179_09790"
     CDS             4945..>5868
                     /locus_tag="P5179_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95549.1"
                     /translation="MRLSRGSRIPLIVFVAVHSFAGCAYYNTFYNAERAFEKGERARQ
                     SREALQRAGSPATRRPSQQSSSTRGAAVGDEQSYRTAIEKSAKVLVYHPRSRYVDDAL
                     LLMAKSYYRLGEYAPSLRKCTELLTTFPQSPLAPEARYWEGMALWQLGRYEEATGVLS
                     RIAADDGSRFQGDAAFALAKLKRERGSSEEAIPLYRVAVRRSTEREFRYRARIELAEC
                     LIEAGGMSEAVEVYRELSRLPQTAAERYENLLRIARTQRAMGNAEAALATLEPLIRDQ
                     RFTDQVPRTRLEIARTKEAAGEIEAAVLLYQK"
BASE COUNT         1216 a         1771 c         1734 g         1147 t
ORIGIN      
        1 acaggatcga cggcatgatc attccttcca gacttccgga gaacaagcca agcatcatgg
       61 aagcctggat ttcggcgatt ctcaaggaca cgcccttgat cacgacggtc gaagacgggc
      121 gcaatctgac ggaaatgctc gagggatgtt ataccgcctg gcggacggga aaacgttacg
      181 cgttcgccaa atgagccgtt ctttctcaaa gtccgggaga ggaagctcct tcacgtcccc
      241 tcccggattc cctttcctgt gagcacgttg cgggttgccc ggtggagcca gaacacaacc
      301 gcgccggccc agagccacgc cgcgaggatt tccatcggcc agcttctttt cgcgatcaat
      361 gccgacgcac cagacaggag aacaacaaga acggcccctg tcagcgccgc cttccgccat
      421 cgggtcattc tgcccgtccg cgcaacaaca agagctacgc ttccagccca ggctgaggcg
      481 agtaacacgg cgcttgaggg cacgtccggt accgatgcgc ctgtgctgct gaacatgatc
      541 cctttcagga ggcgatccag tacggcggcg cctccgaggg cggccaacgg aatcacgctt
      601 tccttcgtcg gcaggaacac gtagagcagc aacgtcgtca acgcgatcgc ggcagaggac
      661 acgaaatcat ccgacagtgc gccgccgacg aaaagccaga acggcgactg cgccgcggaa
      721 tgaactatcc cttccgccac cgaccgctcc atttcgctcg gaggcaaatt gacggccgcg
      781 gcagtgacgg ccgccgctat cacggcgtac ggaagccaac tcgtccacct catccgggaa
      841 cctcgggtcc tgaccctgcc agaattgcct ggtcgggttc aaaatacgcg cgaactaccc
      901 tgtttgtgtc ctcagccgtc accgagttca cgaggtcagc aaatcggtgg aggtaatccc
      961 agccgagacg gtatatctcg atctccgaca tgatgcttgc cactccctcg ttcgattcca
     1021 gcttcaaggg gagtgatcca atcaggtgcg atttgcagtc ctccagttcg tcttgcggaa
     1081 cgggtgtctc ccgcagcttg gcgacttcct gttctatggc cgacaccgct tttcggtaat
     1141 gttcgggtgc aacccccgca acgcacaccc aaggcccggc gcccagacca gcgttgacgc
     1201 ggctcgctgc gtaatacgcc aggccgagtt cctctcgcac cttttgaccg agtcgaccca
     1261 taagtccgaa accaccgagg atgagattcg ccacacttgc cggaacgaaa tcaggatggt
     1321 ttcgcggggg gcctatcgtg ccccaggcca ggtcgatctg cactttaccg gggattaccg
     1381 tgtacgtttc ccgcggcccg tcacgacgcg tgcaatccgg caagggcgcg ggatttgcag
     1441 ttctcgcatt cgtgctccag agtggcgcga agagcttttc tacgtcgtcc tgtcgcagat
     1501 tgccgactac gctgatgatt gccggtcgcc gccgaaccag gctgtcgaaa aagtgctgca
     1561 catcgtcccg cgtgaacccg gccacggttt ctctctcacc gtcggcgggg cgtgaatagg
     1621 gatggtcttg cggataggcg agtttgcgaa acgcgaggtc cgtaactgat ctggtgtcat
     1681 tatcgcgttc ctcaagctcc gtgaggacct cccctcgtac cttctcaatt tccgctgcgg
     1741 gaaaggacgg gctgtgaagg acctcggaga gcagctcaac gatcatctgg agatcgtctg
     1801 aaaggcactt tgcgccaaat cgaactgtgt gcaggcccgc cccgacgccc aacgaacctc
     1861 caacggattc caccagttcg aaaagctccg ccgcggaccg gcgctgtgtg ccccgtgtca
     1921 aacattcggc ggtgaaccga cacaatcccg ggcggtccag ggggtcatcc acggcgcccg
     1981 taagaatcgc gccctgaacc accaccgcag ggctggcggg gttctcgcgg acgaggagga
     2041 tggctccatc cggcaacaca gtccgcgaaa cgctttcgga atccgggaaa ccggtcgacg
     2101 ctgcagaggt actcactgga taccttcttc cgtttccgtt tcttcgtccg cgtcttccag
     2161 ctcgtcttcg ccgccgaggg gttgatacca cccgacaacg cgatttcgtc tggcaaacag
     2221 gctttctgcg gctgctctga cgtcttccgc cgtaacatcg ctgatgcgct gctcaaactc
     2281 gtccagaaac tgcacaggaa atacggtgaa tccgagagtc aacaccatgg cgcgatgggt
     2341 gatacgctca tttgtgaagg cgaacagcgc gcgcagctgt ttgcgtgctt tcgccatttc
     2401 cgcagcgtcc ggtggtgagg ccgcgatcgc ttccagctcc cgcagaatcg ctccctccac
     2461 ctcgccgtga gtcctgccgt ctctcacaac agccgtgatg tcaaaaagga acgggtcgat
     2521 acttgcttcc aggttgcagt cgacgtcgat tgccagttcc ttttcgacca acgcgcggta
     2581 caggcgggaa ctgcgactct cagctccgct accgaagccg agggagatcg ggcttccaag
     2641 gatggagccg agaacaagcg acggcacaaa acggggatca gttgcttttg gcgcaagaaa
     2701 cgccatttcg aggtagcccg tcgcacccgg gccctcaagc agaacgcggc gctccgcgac
     2761 cggggctggt tcaatggcgc gaggtgcagg aggcgtcgca ccggggggaa tttccgagaa
     2821 aagctcttcg agacgcccta ggagaacccg cgtttcgaaa tctccgacta caacacacac
     2881 ggcgttgttc ggaatgtaat acgtccgaaa atacgcgagc aggtcctcgc gcgagagatt
     2941 tcggatatcc tcggcgatgc cgatcacctc gtgcccgtag gggtgcgcgt gaaatgcagc
     3001 agccgtaacc tgctcggcaa gtcgccattc cggttggttt tcatacgcat cgcgctccgc
     3061 gagtatgaca gagcgttccg tttcaaaaac atccggagcg aacaacggac ctgaaagacg
     3121 gtctgcctcg atctgcagga caatgtccaa cttggaggac gggatcgtcg tgtagtatgc
     3181 ggtgcagtct atccatgtga aggcattggt ggttcctcct tcgcgtgcga tcaagcgatc
     3241 acgctcaata cccgggaatc gctgggaagg tttgaacagc atatgctcga tccaatgaga
     3301 taccccggtc tgcccgggga gctcattccg gctccccacg cggtacccca tccagaaact
     3361 ggttaccggc gcatgatgcc gctcctccgt taccacggtc agaccgttcg tcagcacaga
     3421 cacttgatac ggcgggttcg ccattcggtt ctccgttccg cgtcagggac gaacgtgagg
     3481 gccgaacacg cggcgcagat aaatacgatt gcacgcgcgt ccctgatcga accgcaggtc
     3541 cacgatgtcg aaatcctcgg tgatacagag ggtcaacatc ccgcgatacc gattcagcgt
     3601 attgatgtca acggagcggt accctcgcct gcgtatctcc tccagggtga agcggaaaag
     3661 ccccctcccg atgcctttgc cgcggatttc gggaaggcct cccatgagag cgatgaagaa
     3721 gacgccgggc tcctgtgcgt catgacccag cacgtacgtg acgattctgc cgtccagcct
     3781 cgctacggtc agcaaggctc ccggtttttc cgtgagagtc tgcctccacg aggcagcgcc
     3841 cttttgcagg tccttcgcgc cgtcccattc gtaaaatgcg gcttcgtgga ccttcaccgc
     3901 ctcatcaacc gcctcgacac ccgctatctc gtataccggt tcgctcacgt ctgtttcccc
     3961 cgtccgttgt gtggataacg acttgagaaa aaatacgcta acccacctgc gccccaaagg
     4021 gcggcctgtg cttgacatgc gtaggatgga tggtgcactc ttgataacgt gggtatagat
     4081 acttccccga ttagtcgggg gttcgcagaa cgatggacaa gggacaagag cagctcatgt
     4141 gcgataacac ggatatcatg gagtcggtga aggaaatagc cgaggaggcg ggccagatcg
     4201 cgctttcgca attcggcgcc agccggcctc acgtgaaaac tgatcggagc ttcgtcactg
     4261 caacggaccg ggaagtggag cagttcatac accggaaact tcacgagctc ttcccggacg
     4321 acgccatcat cggggaggaa ttcggtgggt cccctggcac catacgggaa tacgtctggt
     4381 tactggatcc catagacgga accaccaact tcgttgcggg cttgccccat tgggcagtct
     4441 gtgttgcacg gctgcggttt ggcgttcccg tgctgggagt ggtcgtcctc cccgtcctgc
     4501 gcgagaccta cgccgctgca acgggaatcg gcgcaacgtt gaacggctcc ccgttcaaaa
     4561 tgctcgaacg tgacgatccc ccgaacgaac aactgatagc cgtatggtcg acgtggtacc
     4621 gggaagtgga cctcaagttt tcggggaagg tccgaactct tgggagtacg gtcgtcaagt
     4681 gcctttacgc cgccagaggc agtttcgttg gcgctttgac accggaggtc cacgcatggg
     4741 atatcgccgc cgggcttccc gtcctctggg aatccggtgg tcaggcgtac gcgagggatg
     4801 gtaaggtcta cgaggtgatg gatctcgatc ctgcgaacgg atacctcgtt ccgccgctca
     4861 ttctcagcgc cccttcgcgg agggatttta tgcggcagat gatcactgcc aggcgaacgt
     4921 gatgcatcgg gtgggacgat aggaatgagg ctctcgcgcg gcagcaggat cccgctgatc
     4981 gtcttcgtgg cggttcacag tttcgccggg tgtgcgtact ataacacgtt ctacaatgcg
     5041 gaacgtgcgt tcgaaaaagg tgaacgcgcc cgacaaagcc gtgaagcact gcagcgggcc
     5101 gggtcccccg cgacccggcg cccttcccag caatcatcgt cgacgagagg ggcggcagtc
     5161 ggcgatgagc aatcgtaccg caccgcaatc gaaaaatcgg caaaggtgct tgtctatcat
     5221 ccgcgaagtc ggtatgtaga cgacgcgctg ctgctcatgg cgaaatcgta ttaccgtctg
     5281 ggggagtacg caccttcgct gcgaaagtgc accgaattgt tgaccacgtt cccgcagagt
     5341 ccgctggccc cggaggcgcg gtactgggag ggaatggctc tgtggcagct tggaagatac
     5401 gaagaggcca cgggtgtgct ctcccgcatc gccgccgatg acggatctcg gtttcagggt
     5461 gacgcagcat ttgctctggc gaagctgaaa agggagcggg ggtcttcgga ggaagcgatt
     5521 ccgctgtacc gcgtcgcagt acgtcgatcc accgaacggg aattccgcta ccgggccagg
     5581 attgaactgg cggaatgcct gattgaggcg ggggggatgt cggaagctgt ggaggtgtac
     5641 cgcgaactga gccgcctccc gcagactgca gcagaacgat atgagaacct gcttcggatt
     5701 gcgagaacgc aacgtgccat gggaaatgca gaagccgcgc tcgcaaccct cgaaccgctg
     5761 attcgggatc agcgtttcac cgatcaggtg ccgcgtacga gactggagat cgcaaggacg
     5821 aaagaagcgg cgggcgaaat cgaagctgct gttcttctgt accagaaa
//
LOCUS       DAOTNG010000197         5840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11639_length_5840_cov_5.244944, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000197 DAOTNG010000000
VERSION     DAOTNG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11639_length_5840_cov_5.244944"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="P5179_09795"
     CDS             complement(<1..370)
                     /locus_tag="P5179_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS95550.1"
                     /translation="MHIDPECPVDLHTHTTASDGSLSPEDLVDLARQGGLCAIAVTDH
                     DTTDGVIRAIEAGQRAGVVVLPAVEISSQHKPGSMHILGYFIDVGEPHLQDTLAEMVR
                     NRDRRNRGIIERLNNLGVHIT"
     gene            399..1517
                     /gene="queG"
                     /locus_tag="P5179_09800"
     CDS             399..1517
                     /gene="queG"
                     /locus_tag="P5179_09800"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRS95551.1"
                     /translation="MGSDVLSILRRHAQAHGLVGPFVAPKLTPDTWESFCKWLDAGFA
                     GDMVYLGRRRNERRDVRYLNPRARSLAVFALPYSCASPNTETMHSGLISPHAMGEDYH
                     HRCRNALKRVVDALRTELGTHVDGWIHVDTAPILERDFARQAGLGWVGKNTLLINPDF
                     GSRFHICEIALSIDIPATPVVVPDGCGACTRCLDSCPTGALVAPRVLDARRCISYLTI
                     ESKGSIPRDLRPALATRLFGCDACQEVCPWNQKATVRPFSDAATLAMDVIEILSLDEE
                     SFRSRFRSSPFERTGRTRLLRNAAVVAGNIRAQKATGPLLHLLKDTEPLLREHAAWAL
                     ARVRGPEMAEAIQSAYERETDARARSDMALSLRDARES"
     gene            1642..2973
                     /gene="der"
                     /locus_tag="P5179_09805"
     CDS             1642..2973
                     /gene="der"
                     /locus_tag="P5179_09805"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRS95552.1"
                     /translation="MSRPVVAIVGRPNVGKSSLFNRVVGHRHSVVDPEPGVTRDRIMA
                     KTDWNGREFLLVDTGGLVPGSSDPMEAHILDQVQIAMEEASLVLFLTDAQTGVTDLDA
                     EVARVLRKKERQTLLVVNKVDNPEADVEVHAFHALGMGSPVPVSAMTGRHVGDMLDQL
                     VKRLPPEAEGEEPEGGIAVAFVGRPNVGKSSLVNALIGSPRVVVDDVPGTTRDATDTF
                     FQHEGRSYTLVDTAGLRRQKNVWKTRDAIEHFSVLRTLKAIERCDVAVLVIEAVDHLV
                     KQDSDILAQIVDAGKAVVIAANKWDAVPGKTSETAGAFMKELWVRNPFTEHVPVSLIS
                     AATGQRVEKVFSDVLTAYEQWTRRIPTNTLNEWLRELVKKNPPPISRRGVPRLSYVTQ
                     VGVRPPTFVFFVNNPDAIGTAGERYLERCLREEYGFSGTPLRLLFRKKSRP"
     gene            complement(3042..4439)
                     /locus_tag="P5179_09810"
     CDS             complement(3042..4439)
                     /locus_tag="P5179_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRS95553.1"
                     /translation="MKRGVILFVCVVISVGTAWSANWNTRRNPKNVIILVGDGMGFNQ
                     VEAGSLFSGRQVFERFPVRYGCTTFSASGNGYQPDSAWTSFDYLKKRATDSGAAATAL
                     ATGTKTTNGAIGVGPDGDPVPNILEVAEDVGKATGVVSSVGISHATPAGFVAHSRSRG
                     SLESIAQEMILRSRVDVIMGAGNPWFDDNGNALAEPKNFRWVGGEQVWKGLLLSGARF
                     DLDTNGTYETTVADADGDGVPDRWELVQTRRAFRQLAAGPTPKRVLGLPQIHETLQQK
                     RTDQRGSNLRQDYANDAPYAVPLNEKVPTLAEMTRAAINVLDNDPDGFVLMVEGGAID
                     WAGHANLSGRLIEEVAGFTEAIEAVVAWVDSASSWDETLVVVTADHETGYLWGPGTGF
                     GADGAIQWNPLQGQGLRQMPAMQWNSTGHTNSLVPFFAKGRCSERFARFVDGTDPRYG
                     QFVDNTAIFRMLTED"
     gene            complement(4552..5409)
                     /locus_tag="P5179_09815"
     CDS             complement(4552..5409)
                     /locus_tag="P5179_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS95554.1"
                     /translation="MTTLKSRKVFWLWCMALGWVLPANLTGEALPARNDLQLDEMVRI
                     PATSFLMGDDHSDADVRPAHRVDVSAFFIDRREVTCEEYRRFLLANPEWRKGRVSPSL
                     ADRNYLRDWDDLSYPRGKAAHPVTWVSWHAANAYAKWRGARLPTEAEWEATARGATGR
                     IYPWGNEDPENSEMPRANYASARGRFGRHGGTEPVGRYPAGATPEGVLDMAGNVWEWV
                     ADWHDPDYYSESPRVNPTGPSNGSYRVVRGGSWAVPSPWLRSYARLRAFPAKTSDQVG
                     FRCARTSSP"
     gene            5649..>5840
                     /locus_tag="P5179_09820"
     CDS             5649..>5840
                     /locus_tag="P5179_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRS95555.1"
                     /translation="MTGSNLSLPPWFVSEQPQPLILASASPRRAELLHQVGIRFLQEP
                     VEVDETWPKDLLPDEAVRSL"
BASE COUNT         1206 a         1766 c         1641 g         1227 t
ORIGIN      
        1 gcgttatatg aacccccagg ttgttgagac gctcgatgat gccacggttt cgccggtcgc
       61 gattgcgtac catttccgcc aatgtatctt gaaggtgggg ttcaccgacg tcgatgaagt
      121 agccgagaat atgcatgctt cccggtttat gctgcgaact gatttcgact gccggtagta
      181 ccaccacacc ggctcgttgg ccggcttcta tggcacggat gacgccgtcg gtggtatcat
      241 ggtcggttac tgcaatcgca cacagccctc cctgccgggc caggtctacc agatcttcag
      301 gtgacagtga accgtcggaa gcagtggtgt gcgtgtgcag gtcaaccgga cattctgggt
      361 caatatgcac caaataattc ctttccctgg gagaacctgt gggcagtgat gttttgagca
      421 ttctacgccg ccatgcgcaa gcgcatgggt tggtcggtcc gtttgttgct cccaagctga
      481 cccccgacac atgggagtct ttctgcaaat ggctcgatgc cggtttcgcg ggagatatgg
      541 tctacctggg ccgccggaga aacgaacggc gcgacgtgcg atacctgaac ccgcgtgccc
      601 gttccctcgc cgtttttgcg ctgccgtact cctgtgcctc gcccaacacg gagacgatgc
      661 acagcggact catttccccg cacgcgatgg gtgaagacta ccatcatcgt tgcagaaacg
      721 cgttgaaaag agttgtcgac gcgctgcgca cggaactggg gacgcacgtt gacggatgga
      781 tccacgttga caccgctccg atactggagc gtgatttcgc aaggcaggca ggcctcggat
      841 gggtcggcaa aaacaccctg ctcatcaatc cggatttcgg ttcccgattc cacatctgtg
      901 aaatcgcgct ttctattgac atccctgcaa ccccggtcgt cgttccagat gggtgcggcg
      961 catgcacccg ttgccttgat tcgtgtccca caggtgcttt ggttgcgcca cgggtgctgg
     1021 atgcccgccg atgcatttcg tatctcacca tcgaatccaa aggcagtatt ccccgcgacc
     1081 tccgacccgc tctggcaacg cgacttttcg gatgcgacgc atgccaagag gtgtgccctt
     1141 ggaatcagaa ggccactgtg aggcccttct cggacgccgc aacattggca atggacgtaa
     1201 tcgaaatcct gagtctggac gaagaaagct tccgcagccg ctttcgcagc tcgcccttcg
     1261 agcgcaccgg ccgaacgcgc ctgctacgga acgcggccgt ggtcgcgggg aatatcagag
     1321 cacagaaggc cacgggtccg ttgctgcact tactgaaaga taccgaacca ttgctccgtg
     1381 aacatgcggc gtgggcgctg gcgcgcgtgc gcggaccgga aatggccgaa gccattcagt
     1441 cggcatacga aagagaaaca gacgcgaggg cgcgctcgga tatggctctt tccctgcgcg
     1501 acgcacgtga atcgtaagga acggatcgac tgccctcccc gcgctgtttc atcccggcgc
     1561 cgggaaacac actggatgga cagacaaacc cgtatctttc tttaagcccc cgccgaaaaa
     1621 gaaaccacga atggagtacg aatgtcccgt cctgttgttg cgatcgttgg aagaccgaat
     1681 gtaggcaaat cctccctttt caacagagtc gtcgggcatc ggcactcagt cgttgatccc
     1741 gaaccgggtg ttacccgtga caggatcatg gcgaaaaccg attggaatgg cagagaattc
     1801 ctgttggtcg ataccggagg cctcgtaccg ggaagcagtg acccgatgga agcgcacatc
     1861 ctcgaccagg tccagatcgc gatggaggaa gcttctctgg tgctttttct cactgacgct
     1921 cagaccggag tcaccgatct ggacgcagaa gtcgcgcgcg ttttgaggaa aaaagagcgg
     1981 cagaccctgc tggtagtcaa caaagttgac aatccggagg ccgatgtcga agtccacgcc
     2041 ttccacgcac tcgggatggg aagccctgtg cctgtctccg ccatgaccgg ccggcatgtc
     2101 ggggacatgc tggatcagtt ggtgaaacga ttgccgccgg aggcagaagg tgaggagccg
     2161 gaaggcggga tcgcagttgc gtttgtgggc cggccgaacg tgggcaaatc gtctctcgtg
     2221 aatgcgctga tcgggtctcc cagagtggtt gtggacgacg tcccgggcac cacgagagac
     2281 gcgaccgaca cattctttca gcacgagggc agatcctaca cgctggtcga cactgccggg
     2341 ttgcggcggc aaaagaacgt ctggaaaacc cgcgacgcca tagaacattt cagcgtgttg
     2401 cgcactctca aagcgatcga gcgctgcgac gtggcggtac tggtgatcga agcggtggac
     2461 cacctcgtca aacaggacag cgacatactc gctcagatcg tggatgccgg gaaagcggtc
     2521 gtcatcgccg cgaacaaatg ggatgcggta ccgggcaaga catcagaaac cgccggtgca
     2581 ttcatgaaag agctatgggt gcggaacccg ttcaccgaac atgttcccgt ctctctgatc
     2641 agtgccgcga ccggccaacg cgtcgagaag gtgttctccg atgtcctcac tgcgtacgag
     2701 caatggactc gaaggatacc cacgaacacg ctgaacgaat ggctcaggga actcgtcaaa
     2761 aagaaccctc cacccatttc ccggagaggc gtcccgcgcc tttcgtatgt gacccaggta
     2821 ggtgtgcggc ccccgacctt cgtgtttttt gtgaacaacc cggacgcaat cggtaccgcg
     2881 ggggaacgat atctcgaacg atgtctgcgt gaagaatacg gcttttcggg tactccgttg
     2941 aggcttttgt tcaggaaaaa gtcgagaccc tgagacaagg tgcaccggtc tcagggtctc
     3001 tggcattgcc caccaaacgc ggatacgtgc agaactcccg gctaatcctc tgtcaacatg
     3061 cggaagatcg cggtattgtc cacgaactgc ccgtaacgcg gatcggttcc atcaacaaac
     3121 cgggcaaaac gttcgctgca tctccctttt gcgaagaacg gaacgaggct gttcgtgtgg
     3181 ccggtgctgt tccattgcat cgcgggcatc tggcgcagtc cctggccttg caatgggttc
     3241 cactgaattg ctccgtcggc cccaaaccct gttcccggtc cccacaggta tcccgtctcg
     3301 tggtcggcag tgacaaccac caaggtctcg tcccagctgc ttgccgaatc tacccatgct
     3361 actactgctt cgatcgcttc cgtgaagcct gccacctcct cgatgagccg tccgctgagg
     3421 ttggcgtgtc cggcccagtc aattgcgcca ccctcaacca tcaacacaaa accatcggga
     3481 tcgttatcaa gaacgttgat cgcagctcgc gtcatttccg caagggtggg aaccttttca
     3541 ttcaaaggca ccgcataagg cgcgtcgttc gcgtagtcct gcctgaggtt cgatccacgc
     3601 tgatccgtgc gtttctgctg caacgtttca tgtatttgtg gcaagccgag gacccgtttc
     3661 ggtgtcggcc ccgccgccag ttgccgaaat gcccgccgcg tctggacgag ttcccatcta
     3721 tccgggacac cgtctccgtc ggcatctgct accgttgtct cgtacgttcc gttcgtgtcc
     3781 aggtcgaagc gcgcgccgga taacaaaagc cccttccaca cctgttctcc tccgacccac
     3841 cggaagtttt tcggctctgc cagcgcattc ccgttgtcgt cgaaccatgg gttgcccgcc
     3901 cccataatca cgtccacccg gcttctgagg atcatctcct gggcgatgga ttcaaggctt
     3961 cccctgctcc ggctgtgggc cacaaacccg gccggagtcg cgtgggatat gcccaccgaa
     4021 ctcaccacac ccgtcgcctt tccgacatcc tcggcaacct cgagaatatt cggaacagga
     4081 tcgccgtcag gcccgactcc tatggcgccg ttggttgttt ttgttccggt cgcaagcgcg
     4141 gtcgccgcag ccccggaatc tgttgctctt ttcttcaagt aatcgaacga agtccatgct
     4201 gaatccggtt gatatccgtt cccggaagcg gaaaacgtgg tgcatccata tcgcaccggg
     4261 aaacgttcga aaacttgtct gcccgaaaaa agagaacccg cctcaacctg gttgaaaccc
     4321 atgccgtcac cgacaagaat gatgacgttt ttgggattgc gccgcgtgtt ccagttcgcg
     4381 gaccatgcag ttcccacgga aatcacgaca caaacgaaca aaataactcc ccttttcacg
     4441 actgatcctt tcggctttgg ccaatgaagg agaacccgcg agcaatcccc aaatgcattc
     4501 tggttgtgcg agggatcaat cgaggttcac aactgcacat gatacagaag atcagggcga
     4561 agaggttctg gcgcaacgga aacccacttg atcgcttgtt ttggcgggga atgccctcag
     4621 cctggcgtac gaacggagcc agggcgaggg aactgcccag gacccgccgc ggacgacacg
     4681 atagcttccg tttgatggac cggtaggatt cactcgaggg ctttcggagt agtaatccgg
     4741 atcgtgccag tccgccaccc attcccacac gttgcccgcc atgtcaagta ccccttccgg
     4801 cgttgcgcca gcgggataac ggccaacggg ttccgtccca ccgtgccggc caaatctgcc
     4861 gcgtgcagac gcgtaattcg cgcgcggcat ttctgagttc tccgggtctt catttcccca
     4921 cgggtagatt cgtcctgttg ccccgcgcgc ggttgcttcc cactccgctt cagttggaag
     4981 acgcgccccg cgccacttcg catacgcgtt ggccgcatgc cagctgaccc aggtcacagg
     5041 gtgcgcagcc tttccccggg ggtatgacag atcgtcccag tctcgaagat agttcctgtc
     5101 cgccagagag ggtgagactc gccccttgcg ccattctggg ttcgcgagaa gaaaccggcg
     5161 gtactcttcg cacgtaactt cccgccgatc tatgaagaac gccgagacgt cgacgcgatg
     5221 tgccggacgt acgtcggcgt cggaatgatc atctcccatc aaaaacgaag tggccggaat
     5281 acgcaccatt tcatcaagtt gcaagtcgtt ccgcgcggga agggcttcac ctgtcaaatt
     5341 ggccggcagt acccaaccca acgccataca ccacagccag aaaaccttac ggcttttgag
     5401 cgtggtcacc tctcttccct tccgcgccat tttcgtgcca gtgacacgcc tgtttcgtat
     5461 ctttttgaac gactggatcg ggacaaggca atcaaccaca cctggtccga ccggtccgtt
     5521 tgcagacaaa tgtacgcgcc gtttcagttc atggtgaacg gcggcagaca ccttcggaaa
     5581 ccgccgcgca tggattcgtg tgtgtggcgg gcccgcacgc gcaagcatcg tggcatcgga
     5641 aattacgaat gactggttct aacctttccc taccgccatg gtttgtgtcg gaacagcctc
     5701 aacctttgat tctggcctca gcgtctccgc gccgcgccga gttgctccat caggtcggaa
     5761 ttcggtttct gcaggagcct gtggaggtag atgaaacctg gccgaaggac ctgttgcctg
     5821 atgaagcggt caggtcgctg
//
LOCUS       DAOTNG010000198         5840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11640_length_5840_cov_4.825929, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000198 DAOTNG010000000
VERSION     DAOTNG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11640_length_5840_cov_4.825929"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2609)
                     /locus_tag="P5179_09825"
     CDS             complement(3..2609)
                     /locus_tag="P5179_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRS95556.1"
                     /translation="MVYCFRALLICLLFSTSWASSGDTTLVVWGIGEGEGQVGFFAAV
                     EAFERANPGVRVSTSPSGGGQNPQKMLTAIVGGAAPDVMNQDRFTVSSWAARDAFLPL
                     DGRLAAQDTTAPDAIRKSEYYTACWNETVFRGKVYAIPNGTDARALYYNFAEFRRVGL
                     VDSAGVPTPPRTWRELGLFSLRLAVNDALENAGLLDADGNLIPPATWRDVEVFNLLLG
                     MPDAEARARMLDDPRHLVLPPEWTSGMVNSYRFPVTQKGLESGIINPEGKMKPPAEWK
                     FRRIGFIPVYGNVWLHMYAWQNHGKFMSPDGRTCTLNDPRIVEALEWMAWFYDMFGGR
                     PRVEAFSSSFQGQFLDPFLTGFLAMKVETDGMLGAIARYKPDLEFGVCLTPLPEGGKP
                     TTWSGGFSWAIPRNARHPDIAWRFIRHMVNYETVRLVERTRMNFNRSRGRPYVMGIPA
                     NRETGRKLAEEFLDANPDLPQRLKDGYSQYFAMMEFSDFRDVTPASQLLWDEIQRATN
                     MATYRKLTPKEAADAGAATVQQELDRLFSSSAGEPVNWTAVALVVLLLVAGGGAAGYF
                     YSREKLHLGRMGRADLVAGLGFISPWLAGFFIFTVGPFIISIVLSFTEYNMLSPARWV
                     GLKHYSELFTNDPIFWKSLGNTIYMMIGVPLGMAVGLGVAMLLNSNVRGMSWYRTVYY
                     LPAIVPTVASSILWIWVLHPTNGILNIILEYVGLGQPLWLQSEHWSKPAIIVMGLWGA
                     GSSMIIWLAGLKGIPDHLYEAAQIDGAGKWRQFVHVTLPMLSPYIFFNLIMGVIGTLQ
                     IFAQAFIMTSGGPVDSTLFYVYYLFNNAFQYLRMGYASAMAWILFVIILGLTLVQLKL
                     APRWVHYEGGGK"
     gene            2699..3556
                     /gene="folP"
                     /locus_tag="P5179_09830"
     CDS             2699..3556
                     /gene="folP"
                     /locus_tag="P5179_09830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRS95557.1"
                     /translation="MKEPFDSLLHPGRTLVMGVVNVTPDSFSDGGRFLSPEAAIAHAC
                     RLMDEGADLLDIGGESTAPDSPPVDAQEECRRILPVLEECAKRGFPVSVDTYKSPVAR
                     CALAHGAGMINDVTALRGDPTLADVLAESDCAIVLMFAKDPTPRTTPTVRVYADVVRE
                     ISEFLAERVAYAGTRGIAASRIVLDPGMGAFVSADPGPSLEILRRAGEFHTLGHPLLI
                     GASRKSFIGKVLGGPGQPLPVGDRLEGSLACAAIAAWHRVQIIRAHDVRETVRTVRMV
                     DAIRVADAV"
     gene            3739..3999
                     /locus_tag="P5179_09835"
     CDS             3739..3999
                     /locus_tag="P5179_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRS95558.1"
                     /translation="MPELSRFFGITVRMYYNDHEPPHFHVFYGDFEALIEVGSLHVFR
                     GSLPRRAFAMVVEWAIIHREELVGAWQSARRGLAVPPIVPLD"
     gene            4011..4307
                     /locus_tag="P5179_09840"
     CDS             4011..4307
                     /locus_tag="P5179_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRS95559.1"
                     /translation="MSMQRIKAVEPLDGFKLKLTLTDGSVIERDVSRLLVGKVFEPLL
                     ASRTEFCRVRAEAGTVVWENGADLCPDVLIWGGPPPQDERPGIANPPVSELKPA"
     gene            complement(4449..5456)
                     /locus_tag="P5179_09845"
     CDS             complement(4449..5456)
                     /locus_tag="P5179_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95560.1"
                     /translation="MTGKIRWGIMSTGKIAHKFAQGLKELPDEAEILAVGSRAQQTAD
                     AFGDEFGIPRRYASYEALVADADVDVVYIGTPHSFHYDNAVLCLNAGKAVLCEKAFTI
                     TGREAEELIALARSKRLFLMEAMWVRFLPATIRVRELVSGGAIGEPRLIQASLGFWTE
                     MTPETKLSRFLNPALGGGSMFDVGIYPMSYISMLFGQARRVTGVAHIGETGVDEQASV
                     CYGYDDGRIASAVFGVRTRVPGGAFIAGSAGTLTMEPSFLNPKTLVLTEAGKDPVRID
                     VACEGTPYRFEAQEVMRCLREEKTESAVMPLDESLAVMRTMDELRGQWGMVCPNDARW
                     G"
     gene            complement(5478..>5840)
                     /locus_tag="P5179_09850"
     CDS             complement(5478..>5840)
                     /locus_tag="P5179_09850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95561.1"
                     /translation="LLHSSGGNFVTEVADISKRYPEISFLLAHSGGDYPTARERLPLV
                     KERENVYLEITLTPVTYRIIEYMVKHVGAEKVLFGTDAPMRDPIPQFGWMCYSRCSEE
                     ELELMLGKNMERILARCR"
BASE COUNT         1114 a         1984 c         1649 g         1093 t
ORIGIN      
        1 tcctacttcc ctccgccctc ataatgtacc cacctcggcg cgagcttgag ttgcaccagc
       61 gtcagcccca gaatgatgac gaacagaatc cacgccatcg ccgaagcgta ccccatgcgc
      121 aggtactgga acgcgttgtt gaacaggtag tacacgtaaa aaagcgtgct gtccaccgga
      181 ccgccggagg tcatgatgaa cgcctgtgca aagatctgca gcgtaccgat cacgcccatg
      241 atcaggttga agaagatgta ggggctcaac ataggcaggg tgacgtgcac gaactgcctc
      301 cacttccccg cgccgtcgat ctgcgccgcc tcgtagaggt ggtctggaat ccccttcaac
      361 cccgccagcc agatgatcat acttgatccg gcaccccaca gacccatcac gatgatggcg
      421 ggcttcgacc agtgctcgct ttgcagccag agcggctggc ccagcccgac gtattccagg
      481 atgatgttga ggatcccgtt cgtcggatga agcacccaga tccagaggat ggaagacgca
      541 accgtcggca cgatggcggg cagatagtac accgtgcggt accacgacat tccgcggaca
      601 ttgctgttca acagcatcgc cacgccgagt ccaacggcca ttccgagcgg cacgccaatc
      661 atcatgtaaa tcgtgttccc gagcgatttc cagaagatcg ggtcattggt gaacagttcg
      721 ctgtagtgct tcagacccac ccagcgggcc gggctcaaca tgttgtattc cgtgaagctg
      781 agcacgatgg aaatgatgaa cggccccacc gtgaatatga aaaaccccgc cagccacgga
      841 ctgatgaaac ccagccccgc cacgaggtcg gcacgcccca tgcggccgag atggagcttt
      901 tcgcggctgt agaagtaacc ggcagcgcct ccgcccgcaa ccagcaacag caccaccagc
      961 gccacagcgg tccagtttac gggctcaccc gccgacgaac tgaacaaccg gtcgagttcc
     1021 tgttgcaccg tggcggcgcc ggcgtccgcg gcttctttcg gcgtgagttt gcggtacgtc
     1081 gccatgttgg tcgcgcgctg aatctcatcc cagagcaact gggaagccgg tgtcacgtca
     1141 cgaaaatcag agaattccat catcgcgaaa tactgggagt acccgtcctt gagccgttgc
     1201 ggcaggtcgg ggttcgcgtc gaggaactcc tccgcaagct ttcggccggt ctccctgttc
     1261 gccgggatgc ccatcacata gggcctcccg cggctgcggt taaaattcat ccgtgtccgc
     1321 tcaaccagtc gcacggtctc gtagttgacc atgtgccgga tgaaccgcca cgcgatgtcc
     1381 gggtgccgtg cattccgggg aatcgcccac gagaacccgc cggaccaggt ggttggtttg
     1441 ccaccttcgg gaagaggagt caggcagaca ccgaactcga ggtcgggctt gtagcgggca
     1501 atcgcgccca gcatcccgtc cgtttccacc ttcatcgcga gaaatccggt gagaaacgga
     1561 tcgagaaact ggccttgaaa actcgagctg aatgcttcga cccggggacg gcctccgaac
     1621 atgtcgtaga accaggccat ccactccagc gcctcgacga tgcgcggatc gttcagcgta
     1681 caggtccgtc cgtccgggct catgaacttc ccgtggttct gccacgcgta catgtgcagc
     1741 cacacgttgc cgtacacagg gatgaagcca atccgccgga acttccactc cgccggcggc
     1801 ttcatcttcc cttcggggtt gatgatcccc gattcgagcc ccttctgcgt aacgggaaag
     1861 cgatatgagt tcaccatccc gcttgtccat tccggtggca gcacgagatg ccgcggatcg
     1921 tcaagcatgc gcgcacgggc ctccgcgtcg ggcatgccca gcagcaggtt gaacacctcc
     1981 acgtcgcgcc atgtagccgg cggaatcaga ttcccgtccg cgtcgagcaa cccggcgttc
     2041 tccagcgcat cgttcaccgc aagccgcagg ctgaaaagcc ccagttcccg ccacgtgcgc
     2101 gggggcgtcg gcacgcccgc ggaatccacc agccctacgc gccggaactc cgcgaagttg
     2161 tagtagagtg cgcgcgcatc cgtcccgttc gggatcgcgt acaccttgcc ccgaaacacc
     2221 gtttcgttcc agcacgccgt gtaatactcg ctcttgcgta tcgcgtccgg tgccgtggtg
     2281 tcctgcgcgg caagccgccc gtccagcggc agaaaggcat cgcgcgccgc ccagctcgac
     2341 acggtaaaac ggtcctggtt catcacatcc ggcgcggcgc cacccacaat cgcggtcagc
     2401 atcttctgcg ggttctgtcc cccgccggag gggctcgtgg atacccgcac cccggggttc
     2461 gcccgctcga atgcctctac cgccgcgaaa aacccgacct gcccttcccc ctctccaata
     2521 ccccacacca cgagcgtcgt atcgccgctg gacgcccacg acgtggaaaa caacaggcaa
     2581 atcagaagag cgcggaagca gtacaccatg gccctctctt gaccggaagg aaattgcgtg
     2641 cgttcatcat tcgactgaaa tatggcacca cgaccgcgcg cgcggaaagg gcattcccgt
     2701 gaaagaaccg ttcgattccc tgcttcaccc cggccgcact ctcgtcatgg gagtcgtgaa
     2761 tgtcaccccc gattccttct ccgacggcgg caggtttctc tcccccgaag ccgccatcgc
     2821 gcacgcctgc cgcctcatgg acgaaggcgc cgacctcctc gacatcggtg gcgagagcac
     2881 cgcgccggat agcccccccg tcgacgcgca ggaggagtgc cgccgcattc tgcctgtcct
     2941 tgaggaatgc gcgaaacgcg gcttcccggt gtcggtggat acctacaagt cccccgtcgc
     3001 ccgctgcgcc ctcgcccacg gagccggcat gatcaacgac gtcacggccc tccgcggcga
     3061 tccgacactc gctgacgtgc tcgccgaatc cgactgcgcc atcgtcctca tgttcgccaa
     3121 ggacccgacc ccccgtacca cgccaaccgt gcgcgtatac gccgacgtcg tgcgggaaat
     3181 ctcggagttc ctggccgaac gcgtggcgta cgccggaacc cgcgggatcg ctgccagccg
     3241 cattgtactc gaccccggca tgggcgcctt cgtcagcgct gaccccggac cgagcctgga
     3301 aatcctccgc cgcgcgggcg agtttcacac actcggacac ccgctcctga tcggcgcatc
     3361 gcggaaaagc ttcatcggaa aggttctcgg tggccccggg cagcctctcc cggtgggcga
     3421 ccgcctcgaa ggctccctcg cctgtgccgc catcgccgcc tggcacaggg tccagatcat
     3481 ccgcgcgcac gacgtccgcg aaaccgtccg caccgtccgc atggtggatg ctattcgcgt
     3541 agcggacgct gtctgaatgg cgcctcaccc caggtctgct tgacacgccg cgtggagggg
     3601 cccgcttcgt ccgacccttt cttcgcagcg caaccaccgg cacgttgcct tcattctgcg
     3661 cgtgaatttg ttactattca aaacagcagc gtatcttgaa tcaaagacgt acaccaaggc
     3721 ggacccggaa ggttcgaaat gcctgaattg tcacgtttct tcgggattac cgtgaggatg
     3781 tactacaacg accacgaacc tccgcacttc catgtctttt acggtgactt cgaagctctg
     3841 atcgaagtcg gaagcctcca cgtatttcgg ggctctttac cgcggcgggc atttgcaatg
     3901 gttgtggaat gggcaatcat ccatcgagag gagcttgtcg gggcgtggca gtctgcccgg
     3961 cgcggcctcg cggtgccacc gattgttccc ttggattagg agcttggtca atgagcatgc
     4021 agaggatcaa agcggttgaa cctctggacg ggttcaaact gaaactgacc ttgactgacg
     4081 ggtcggtcat cgaacgagat gtttcccgtc tgctggtcgg aaaggtgttt gagcctcttc
     4141 tcgccagccg cactgagttt tgtcgtgttc gtgcggaagc tggcaccgtg gtctgggaaa
     4201 acggtgcgga cctgtgtccg gatgtactga tctggggagg gccgccgcca caggacgaac
     4261 gccccggcat tgccaacccg ccagtatcag agctgaagcc cgcgtagagg tggctgatct
     4321 gtcctatgcg tcccatagga cctgtgccgc acgacaggga aaggcaccgc ccaccccggt
     4381 catcgagtgg cgaacgccgc ggaggtttct ctcctgcggc gttcgttgta tcgagataac
     4441 cgctctgttc acccccaccg cgcatcattc gggcatacca tcccccactg cccgcgcaac
     4501 tcgtccatcg tccgcatgac ggcaagcgac tcatccagcg gcatcacggc gctctcggtc
     4561 ttctcctcgc ggaggcaccg cattacttcc tgagcctcga atcggtacgg cgtcccctcg
     4621 cacgccacgt caatccgcac cggatccttc cccgcttccg tcagaacaag cgtcttcgga
     4681 ttgaggaacg acggttccat cgtcaacgtg cctgcactgc cggcgatgaa ggcaccgccc
     4741 ggcacccgtg tccgcacgcc gaacacggcg ctggcgatgc gcccgtcgtc atagccgtag
     4801 cataccgacg cctgttcgtc caccccggtc tccccgatat gcgccacacc ggtcacccgc
     4861 cgtgcctgcc cgaacagcat ggagatgtac gacatcgggt agatgccgac atcgaacatg
     4921 ctgcctcccc cgagcgccgg attgagaaag cggctcagct tcgtttccgg ggtcatttcg
     4981 gtccagaacc cgaggcttgc ctggatcagt cgcggttcgc cgattgcacc gccggacaca
     5041 agctcccgca ccctgatcgt cgccggcagg aagcgcaccc acatcgcctc catgaggaac
     5101 agccgcttcg accgcgccag cgcgatcagc tcctccgctt cccgcccggt gatagtgaat
     5161 gccttctcgc acaggactgc tttgcccgcg ttcaggcaca ggaccgcgtt atcgtaatgg
     5221 aatgagtgcg gagtgccgat gtagacgaca tccacgtccg catccgccac cagtgcttcg
     5281 tacgacgcgt accgccgcgg gataccgaac tcgtcgccga acgcatccgc ggtctgttgg
     5341 gcgcgggaac cgacggcgag gatttccgcc tcatccggca gctctttcag cccctgagca
     5401 aacttgtgcg cgatcttccc cgtgctcatg atgccccagc gaattttccc cgtcatatca
     5461 tttgtctcca ccggcggtta tctgcatcgc gcaagaatgc gctccatgtt cttgccaagc
     5521 atcagctcca gttcttcctc ggaacagcgc gagtagcaca tccacccgaa ctgcggaatc
     5581 ggatcgcgca tgggtgcgtc cgtgccgaac agcacctttt ccgctccgac gtgtttcacc
     5641 atgtattcga tgatgcgata ggtcaccggc gtcagcgtga tttcgaggta gacgttctcc
     5701 cgttccttca ccagcggcaa ccgttcccgc gcggtggggt aatcgccccc cgaatgcgca
     5761 agcaggaagc tgatctccgg ataacgtttg ctgatgtcgg ctacctcggt gacgaagttg
     5821 ccgcccgacg agtggagcag
//
LOCUS       DAOTNG010000199         5803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11724_length_5803_cov_3.871608, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000199 DAOTNG010000000
VERSION     DAOTNG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11724_length_5803_cov_3.871608"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="P5179_09855"
     CDS             <1..1348
                     /locus_tag="P5179_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS95562.1"
                     /translation="TGVLFYLRCRAKPDAIDGSIGEVRPQRLLQNNVWLSYFNERDKP
                     TCVVQNGVVKIRNPVPVPITLSLPSIAQINAGQPALMPVSVDLLTMRDSVTTVNLNMR
                     LPSGVVFSDVSMLPGELLPDGTVLTTTPVEGGIVVSCVSPHLISGSGELLTVRLTAAS
                     TSPDSVSGPVALERVTSGVGYLDYFVKLKRPVVVSNGVVLVRNLNMVPVHIAVSADSS
                     VDAGSPCSIQVQGSGLISFSGTTTLQFALTVPPGLYQSASVQGGLLPADAVTLADAGS
                     GLFRVSGSVANPAGGTGTLFTVQGLTKQDIPDSTVFAIAIERLSDGGSPLPYVRKGDQ
                     TPDCTVSNGAVTVRSPVSVAYEGVALPSRSELTAYPNPFNAVITFRVHGPLSDGPIDA
                     TLYTVLGTVVCRWSQSTPEWTWDGRDASGVPAASGVYVVVVRNGEKTWQKRVTLLR"
     gene            1556..>3326
                     /locus_tag="P5179_09860"
     CDS             1556..>3326
                     /locus_tag="P5179_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS95563.1"
                     /translation="MRTPWRSLFALVLAFAGTSGAGLPITLSMDEVVGVRGQTVTMPV
                     RITSEPLVAADSIVSGQFDLVFDPTVLVPDTMLSRQASGGTIMSKSMVAANVEPGMLR
                     LAFASADAQPYVGDSVVVRIVFHVIGQHGQESSLEIHNILFNELPGGATEPGMGWVLV
                     RSAPPVFTSFPSAVTVMVGQLMQVQVKAVDDDGAVFYSSEFTQGTQLFSVNGETGVMA
                     YTPVPADVGSYTIRVTAWDVFEISTSREFRLTVLADTTKPVIIGSPSATGITENAATI
                     VWNTDKLSTSMVRYGLTGGAMTEAGNTTLTRQHAVPLTSLLSNRGYSYEVWSVDVFGN
                     SSAVKTGTFTTLQAPQTEPLVITLRPSVERGTNQAAVSWETNRQSTTVVRYGTSSAAL
                     TDSVVGVSGSTHTATIKGLTPNTKYFFRVRSVDAWNLSVLSAIDSFTTKALLLAPVIT
                     TPPSVIARDHTSFTVRWVTDRASTSVIDVGTTAAYGRRISDTTAVTVHIVAVTNLAPS
                     TQYNYRAGSAGPEGLGPTFSANQQVYTTDAPDTKPPVITQGPVDQGVDNESATIYWLT
                     DEAANAIVDYSVVQPDGSADFGQR"
     assembly_gap    3327..3426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3442..>5803
                     /locus_tag="P5179_09865"
     CDS             3442..>5803
                     /locus_tag="P5179_09865"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS95564.1"
                     /translation="MVQPDGSADFGQRVVRMVYEKSHSVVLTNLLPSTTYRYRVSSFD
                     LIGNGPTQAIAEFRTAAAPDLTPPVLLGRVQVSGITERSAMVRWATDEASDSRVLYTA
                     QSAVPEAVTVSALVTDHVVSLTGLAPSTNYTLSVYSTDIAGNPLGPIAASFRTKAVPD
                     TIPPAITALPKVDGQTLTSFNLVNVVMSLGTNEVATAQAVFGFNQAALVGMVPASAPG
                     LTQVLQFTNLPPDTTVFYRITVTDAAETPNSRVDIVRSFRTIKGADLTAPAITVGPVV
                     TDVQERSFTVAWTTDEASTSRLRFGTVATNLVGNAQDPTPVQSHRITATNLTPGTKYF
                     WRVLSADPSGNTVASSVREVTTPVERDITPPVFVRRPVATYAAFDNAVITWATDENTT
                     AYIYYAGSTDTVFNVAVDENYRTDHIVSITNLRGGVEYVYYVEAMDNAGNSAVAGDTS
                     APVFQNNGKITRLGDGTAKITQAPRGGTFTTTVTPDVTYPVITGDPNVVETNSTSTTV
                     TWTTDETSNSVVRILETSGGAGKAARAADITADLLERGRVIAQSDNVKNHQLTITSLT
                     PGASYVLHVTSTDPSGNGETISQPVYVTLPQAADLTPPSIVEGSVTATMTQTQAIISW
                     QTDELSDSRVDYAQGSVTELTLSRVVPDPVKNHQVVLTNLLPDQLYSYRVFSTDIRGN
                     GPASSGIHTFTFRTAAQPDVTPPVISGVTVTGVTDVNANITWQTDEDADAFVEYGTTP
                     SFGLTRNEPVLTKTHSLTLTNLAPSTKYYFRLAGSDRAGNTSALTPL"
BASE COUNT         1138 a         1694 c         1723 g         1148 t
ORIGIN      
        1 caccggggtt ctgttctatt tgcgttgtag agccaaaccg gacgcaatcg acggttccat
       61 tggagaagtg cggccgcagc ggttactgca aaacaacgtt tggctttcct acttcaacga
      121 acgagacaaa cctacttgtg ttgtccaaaa cggggtcgtg aaaataagga accccgttcc
      181 ggttcccatc acgctgagcc tcccgtcgat tgcgcagatc aacgccggcc aacctgccct
      241 catgcctgta agtgtcgatc tgctcacaat gcgagattct gtaacgactg tcaacctgaa
      301 catgcgattg ccttcagggg tcgtgttctc ggatgtctcc atgctgcccg gtgaactact
      361 ccccgatggc accgtgctga caaccacccc ggtcgagggc ggcattgtcg tttcttgcgt
      421 atctccccac ctcatttccg ggtctggaga gttgttaacg gtgcgcttga ccgccgcatc
      481 gacaagtcca gacagtgtca gcggaccggt cgctctggaa agagtgacaa gcggcgttgg
      541 gtatctggat tacttcgtga agttgaagag gccggtcgtt gtctcgaacg gcgttgtcct
      601 agtacgcaac ctgaacatgg tgccggtcca tatcgctgtt tcggcagatt cctcggttga
      661 cgcagggtcc ccgtgttcca ttcaggttca gggcagcggc cttatttcct tcagcggcac
      721 cacgacgctg caatttgctc ttacggttcc gccggggctc taccagtcgg catccgttca
      781 gggagggctt cttcccgcgg acgcagtcac actcgccgac gcagggagtg gtctgttccg
      841 cgtttcggga tcagtcgcca atcccgcggg aggtacgggc accctgttca ccgtgcaggg
      901 gctcacaaag caggacatcc ctgacagcac cgtattcgcg attgcaatcg aacggctgtc
      961 ggacgggggc agcccccttc cgtacgtccg gaaaggtgat cagacaccgg actgcacggt
     1021 atcgaacggc gcggtaaccg tccgaagtcc cgtttcggtc gcgtatgaag gagttgcact
     1081 cccttcgcga agcgagctga ccgcgtatcc gaacccgttc aatgcagtta tcacattccg
     1141 ggtgcacggg ccgctgtcgg acgggcccat tgacgcgacc ctttacacgg tgcttggaac
     1201 ggtggtttgc cgctggtcac agtcgacccc ggaatggacg tgggatgggc gcgatgcctc
     1261 gggcgttccc gcggcaagcg gcgtctacgt ggtggtcgtg agaaacggag aaaaaacctg
     1321 gcagaagaga gtaacactct tgcgctgaag tgcatatatt gtaaagggaa tgcgttcggt
     1381 tcatgatgtc gcgggggcat tgatacgcac ggtcatctgg ccaatgattc catcggaatg
     1441 cgcacagagc gtgaccggaa cttacccggc gaaatggcag ttctgtttcg ttagcgatta
     1501 tcggttttct gtttttccta tgccggctgg cacctgacag cagggagggt agctcatgag
     1561 aaccccttgg agatctcttt tcgcgctggt ccttgcgttc gcgggaacga gtggcgcggg
     1621 tctgcctatc acgctttcca tggatgaagt ggtgggcgtg cggggtcaga ccgttactat
     1681 gccggtgagg atcacgagcg aacctctggt ggcggcggac agcatcgtaa gcgggcagtt
     1741 cgatcttgtg ttcgacccga cggtgctggt accggacacc atgctgagca gacaggcttc
     1801 tggcggcacc atcatgtcga aatcgatggt ggcggcgaat gttgagccgg ggatgcttcg
     1861 tctggcgttt gccagcgccg atgcacagcc ttatgtcggc gattcagtcg tggtgcgaat
     1921 cgtgttccat gtgattggcc agcacggaca ggagagctcg ctggagatcc acaatatcct
     1981 gttcaacgag cttcctggcg gggcgacgga accagggatg gggtgggtgc tcgttcgcag
     2041 cgctccaccg gtgttcacat cgttcccctc cgcggtaacc gtcatggtcg gccagttgat
     2101 gcaggtgcag gtcaaggccg tggacgatga cggcgcggtg ttctacagca gcgagttcac
     2161 ccaggggaca cagcttttct ccgtcaacgg cgaaacgggt gtcatggcgt acacgcccgt
     2221 accggcggat gtcgggtcct acacaatcag agtgaccgcg tgggacgtgt tcgagatcag
     2281 cacgtcgcgg gaatttcggc tgacagtgct cgctgatacg acgaaaccgg tgatcatcgg
     2341 ttcaccttcc gcgacaggca tcacggaaaa cgccgccacg atagtctgga acaccgacaa
     2401 gctttccacc tcgatggtgc ggtacgggtt gaccggtggc gcgatgacgg aggccggcaa
     2461 taccacgttg acgcggcagc atgcggtccc attgacctct ctcctgtcga accgcgggta
     2521 ttcgtacgag gtctggtcgg ttgacgtgtt tggaaacagc tcagcggtga aaacaggtac
     2581 cttcaccacc cttcaggctc cgcagaccga accgctggtg atcacgctgc gcccgtcggt
     2641 ggagcgggga acaaaccagg cggcggtttc gtgggaaacg aaccgtcaat ccacgacggt
     2701 agtgcggtac ggaacgtctt ccgccgcgct gacggacagc gtggtcgggg tttcaggaag
     2761 tacccacacc gcaacaatca aagggctgac acccaacacg aagtactttt tccgcgtccg
     2821 gtctgtagat gcgtggaatc tttccgtgtt gagcgcgatt gactcgttta ccacgaaggc
     2881 gcttctcctt gcgccagtca tcacgactcc cccgagcgtg attgcgcgtg accacaccag
     2941 cttcaccgtt cgctgggtga ccgaccgcgc ctccacatcc gtgattgacg tcggcacgac
     3001 ggcggcgtac ggtcgccgaa tcagcgatac gacggcggtg acggtgcaca ttgtcgcggt
     3061 gaccaacctt gctccgtcca ctcaatacaa ctaccgcgcc gggtcggccg gtccggaagg
     3121 cctggggccg acgttcagcg ccaatcagca ggtctacaca accgatgcgc ccgatacgaa
     3181 gcccccggta atcacgcagg gcccggtaga ccagggagtg gacaacgaat cggccacgat
     3241 ttactggctt actgacgaag cggcgaatgc gatcgtggat tacagtgtgg tacaacccga
     3301 tggaagtgcc gattttggcc agcgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnatcg tggattacag tgtggtacaa cccgatggaa gtgccgattt tggccagcgc
     3481 gtggttcgga tggtgtacga gaaaagccat tccgtagttc tgacgaacct cctgcccagc
     3541 acaacgtatc ggtaccgggt gagttccttc gacttgattg gcaacggtcc gactcaggcg
     3601 atcgcggagt tccgcactgc cgcggcaccc gatcttacgc cgccggttct cctcggccgg
     3661 gttcaggtaa gcgggattac cgaacgcagc gcgatggtac ggtgggccac cgacgaggca
     3721 tcggattccc gtgttctgta caccgcgcaa tcagcggttc ccgaggccgt aacggtttca
     3781 gcgctcgtga cagaccatgt cgtctcgcta actggacttg cgccgtccac caattacact
     3841 ctctcggtgt acagcaccga tatagcgggt aacccgctgg gaccgatcgc tgcatcgttc
     3901 cgcacgaagg cggtcccgga cacgattccg ccggcaatca ctgcactgcc taaggttgac
     3961 gggcagacgc tcacatcgtt caatctcgtg aacgtggtca tgtcgctcgg aaccaacgaa
     4021 gttgctaccg cccaggcggt gttcggcttc aatcaggcgg cgcttgtcgg gatggttccg
     4081 gcgagcgctc cgggactgac gcaggtgctt cagttcacca atctgccgcc ggataccacc
     4141 gtgttctacc ggattaccgt gacggacgcc gcggaaacgc cgaactcgcg ggttgacatc
     4201 gtgcgctcgt tccgtacgat caaaggtgcc gatctcactg caccggcgat cacggtcgga
     4261 ccggtggtga cggacgttca ggaacgcagc ttcacagttg cctggacgac cgacgaggca
     4321 tccaccagca ggcttcggtt cggcaccgtc gccacgaatc ttgtggggaa cgcgcaggac
     4381 ccgactcccg tgcaatcgca ccgcatcacc gcgacgaacc tgactccggg gacgaagtat
     4441 ttctggcgtg ttctgtccgc ggatccttcc gggaacaccg ttgcctcatc cgtgcgcgag
     4501 gtgactacgc cggtagagcg cgacatcact cctccggtgt tcgtccggcg gccggttgca
     4561 acgtatgctg cgtttgacaa cgcggtgatt acgtgggcga cggacgagaa caccacggcc
     4621 tacatctatt acgccggctc gacggatacg gtcttcaacg tggcggtgga cgagaactac
     4681 cgaaccgacc acatcgtcag catcacgaac ctgcggggtg gcgtggaata cgtgtattac
     4741 gtggaggcga tggacaacgc cggtaattcc gcagttgcgg gtgacacttc agctccggtt
     4801 ttccagaaca acgggaagat cacaaggctt ggcgacggta ccgcgaagat cacgcaggcg
     4861 ccgcgtggag gaacgttcac cactaccgtt acgccggacg taacctaccc cgtcatcacg
     4921 ggtgatccga acgttgtcga aacgaacagc acctccacta cagtaacctg gacaacggac
     4981 gaaacctcca acagcgtcgt gcgaattctg gagacgtcgg gcggcgcggg aaaggccgcg
     5041 cgggcggcag atatcaccgc cgatcttctg gaacgcgggc gtgtgatcgc gcaatctgac
     5101 aacgtgaaga accaccagtt gactatcacc tcgctcaccc cgggggcttc gtacgtcctg
     5161 cacgtgacgt ccaccgaccc gtcgggtaac ggcgaaacca tcagccagcc ggtgtatgtc
     5221 acgttgccgc aggccgcgga tcttactcct ccgtcgattg tggaaggctc cgttactgcc
     5281 acaatgacgc agactcaggc gatcataagc tggcagaccg acgagctttc ggacagccgt
     5341 gtggattacg cgcaaggcag cgttactgag ttgacgctct cacgcgtcgt accggatccg
     5401 gtcaagaacc atcaggtggt gctcacgaat ctgcttccag accagctcta ctcctaccgc
     5461 gtgttctcca ccgatatccg cggaaacggg ccggcttcga gcggcatcca tacgtttacc
     5521 ttccggacag ccgctcaacc ggacgtgacg ccgccggtga tttccggggt gaccgtgacg
     5581 ggagtaacgg acgtgaacgc caacatcacg tggcagacag atgaagatgc ggacgcgttc
     5641 gttgagtacg ggacgacgcc ttcattcggc cttacgcgga acgagcccgt acttacgaag
     5701 actcacagcc tcacgctcac caaccttgct ccgtcgacga aatactactt ccgtctggcg
     5761 gggagcgacc gcgcaggcaa taccagcgct cttacgccgt tgg
//
LOCUS       DAOTNG010000200         5801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11728_length_5801_cov_4.601984, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000200 DAOTNG010000000
VERSION     DAOTNG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11728_length_5801_cov_4.601984"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..632)
                     /locus_tag="P5179_09870"
     CDS             complement(72..632)
                     /locus_tag="P5179_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRS95565.1"
                     /translation="MSDYRGHVVAGVVWFLTLAAVAFLLLPELAGSHGGWFVLSGWGF
                     VAQLAVAVLAALWPDVDTASRGRKVFYRVLLAFSLYSMARSQWKMAAVIGLAGILPAL
                     GNHRGWTHTVWAAVLVPLPIVLLPLYVSDDGQWLRTPDFQRISVGLPFYVAAMVGLFS
                     HLAADGFFGLLLARISGGSAKGTRRN"
     gene            complement(719..1372)
                     /locus_tag="P5179_09875"
     CDS             complement(719..1372)
                     /locus_tag="P5179_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95566.1"
                     /translation="MSESMYAEFLASLNDSNQCTRAVLTLAFVWLLVDLIRGAPWAST
                     ALKAVFASSALTTGLFFYPTLAKHTGEHRETLLFWGAMFLLSSALGFADTILGRTQIR
                     LPRSGWPLAVVLVFCTAGIGFPAVEWLGGRTYRAFQAFALAPAPYALVVLPLLAVSAH
                     GSLLGVAWLISVFIASADVALVSRLLGLPYWHPIAVFAVIVSAYFAFRRPISAEHKP"
     gene            complement(1388..4645)
                     /locus_tag="P5179_09880"
     CDS             complement(1388..4645)
                     /locus_tag="P5179_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS95567.1"
                     /translation="MSSLTRLWTFETAGFVGCPAVLPTQDGTLLVCGTNSGHIVAIDS
                     DRNEHWRTPVGSKLGTWVSVSSANDPDRCVLAVTEAGKAIRLSDDGTLVWASDVEAAT
                     SPFNSVGIAFGGHVSRVIVTDAKGRVTGLALDSGEHAWQFHTNVRAVGPAAIGDIDRD
                     GEDEIVFCAGDSFIYCIDLNGRLLWRVKTDAPAEFSAPVLVDFGQGPRVLAGSMDDVL
                     RCITPEGEVAWTCRGAGAGSLEIGIAAGDINADGVPEIVFVHAGRAIQAVASDGTLIW
                     ANTDSGDGDQPFAPSLVDVDRDGRLESLLVQRAGTTLRVFDSEGVQIENYRLEGTMIG
                     APVAADLDGDGMLEIFTVNATTGALSCFKSLGGKTEWPTSRGAFDGRANNLRTGRPLA
                     RKPETPRDLPAVVGPKGRLRLGSNVLDYDLPSGTYASVSLRGSDGVTHFGVTDSQRGV
                     EFEILTCGEYTVQATIYRDADRAPIGAASSQFSVTLFQDEQAEIERLLKHLSGIAGGY
                     GITNDLVRGTRTRKLTWQDILERLPQYEKSSSEQRRAFVEEIRHFLDQLRRDTELQKR
                     RVALTAERDAPTEFLPWIVENPWSAFAPEFDVPAGDCASRVALTTDMRGHDVFVVQIA
                     NLLDVPLEIRASLGRITCPTGQKLDAVAHLTLRRVAWVPTSTSKMGPDALPDLGEAGL
                     IGVPAASSARLWVDVDTGDLPAGEYSTALHLRALTPAGTTWEIPILWTVSSFQLPEQM
                     PVRFCNWGYPHSSPLRDIEETTIRDMQAHHTNVFVITRLPKVEYDNAGNLKGEVDWSE
                     HDWILDRLRPSDMMLISPGGLHPAPDAPGSHTPEWERAFSQYLPLWVNHLAEKGFGYE
                     RWAFYPVDEPGLLGGKLIDELEYQARLFKRIDPRVQVYTDPFKGMTVADLRRVLDVVD
                     IYQLSFGSVLTEPSRERYNYLKTTGRTLWTYEAYGGVKDMVGVKYYWEMIWTAWELGL
                     TGVGYWSYCTRGVDLWQGPNPNNNDWEMVYGGARTPVPSVRVQAIRIGIEDYARMWAV
                     QNAAETARNAGDMNRSKMLTEALEEIVRAARDSEWNPVHVASLRRKLSALAADDT"
     gene            complement(4669..5475)
                     /gene="dapB"
                     /locus_tag="P5179_09885"
     CDS             complement(4669..5475)
                     /gene="dapB"
                     /locus_tag="P5179_09885"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRS95568.1"
                     /translation="MVRIAVCGSAGRMGRRIVALAHEDPELSITGAVEKPGDPAIGCD
                     AGEIAGVGRLSVPVVNNLADAIKGADVAIAFINNPEATVEQAAICRAAAKPLVLGTTG
                     LTSEQEQAFKDAAQGIAVVKTSNFSVGVTVLLRLVEEAAHLLGPRFNCEIVEAHHTLK
                     RDAPSGTAISLAKAVAKTWGWDFEKSVRHGRTGITGERPVDEIGMHSLRAGDIVGQHD
                     VLFGGVGESIELRHRAQSRDTFAMGALRAAKWVVGKPEGLYDMFDVLALR"
BASE COUNT         1209 a         1795 c         1661 g         1136 t
ORIGIN      
        1 agcacgtatc tatcacattg aggcggcccg cgagccgcac attttttttc agtcggggtt
       61 cctttgtgct ctcaattcct gcgcgtacct ttcgcagatc cgccgctgat acgcgccagc
      121 aacaggccga aaaagccgtc agcggccaga tgactgaaaa gccccaccat ggccgccacg
      181 taaaaaggca accccaccga aatcctttgg aagtctggcg tccgcaacca ttgcccgtcg
      241 tccgaaacat acagaggtaa cagaacgatc ggaaggggaa cgagcacggc ggcccacacc
      301 gtgtgcgtcc accccctgtg gtttccaagc gcgggcagaa tccccgcgag cccaatcacc
      361 gcggccattt tccactggct gcgcgccatg ctgtacaaag aaaaagcgag cagcacgcgg
      421 tagaacactt tcctcccgcg gctggcagtg tcaacgtcag gccaaagtgc cgccagcact
      481 gccacggcaa gctgagcgac gaatccccag ccactcagga cgaaccaccc accgtgggag
      541 ccagccagct cgggcagaag cagaaaagcc acggctgcga gcgtcaggaa ccacacaact
      601 ccggcaacga cgtgcccacg gtaatcgctc acgacatgcc ccgggtgcgt ctcggcagtt
      661 ctgtcggcac cctcaacgaa gcaatgtaca tcgtttcctt gctttcgtgc aaatgtggtc
      721 agggcttgtg ttccgcactt atcggccgac gaaacgcaaa ataggctgat acaatcacgg
      781 cgaacacggc gatggggtgc caatacggca acccgagcag gcgggagacc agcgccacgt
      841 cagcggacgc gatgaacacg gaaatcagcc atgcgactcc gagcagcgaa ccgtgggccg
      901 atacagcaag aagaggcagg acaacaagcg cgtacggagc aggagccagc gcaaaagcct
      961 gaaacgcacg gtaggtacgc ccgcccaacc actcgaccgc ggggaatccg attcccgctg
     1021 tgcaaaagac cagcactacc gcgagaggcc agccagaacg ggggaggcga atttgtgttc
     1081 tgccgaggat cgtgtctgca aatccgagcg cgctcgaaag caagaacatc gcgccccaga
     1141 agaggagggt ttcacggtgt tcaccagtgt gttttgcgag ggtcgggtag aaaaacaatc
     1201 ctgttgtgag ggcagagctg gcgaacactg ctttcagcgc cgtgctggcc cacggtgcac
     1261 cccgaatcag atccaccagg agccacacaa aggcaagcgt gagaacagcg cgcgtacact
     1321 ggtttgagtc attgagactc gcgaggaact ccgcgtacat gctttcggac atgtgcgtgc
     1381 gcccgaatca ggtgtcgtcc gccgcgagcg cactcagttt gcgacgcaga gacgctacgt
     1441 gaaccgggtt ccactcggag tcccgtgccg cacggacgat ctcctccaac gcctcggtga
     1501 gcatttttga acgattcatg tcgccggcat ttcgcgctgt ttccgccgca ttttggaccg
     1561 cccacatacg agcgtagtcc tcgataccga tgcgtatcgc ctggacgcgc acgcttggaa
     1621 ccggcgtgcg tgctccgccg tacaccatct cccagtcatt gttgttcgga ttcggcccct
     1681 gccacaggtc caccccccgg gtgcagtacg accagtagcc gacaccggta aggccgagtt
     1741 cccacgccgt ccagatcatc tcccagtaat acttcactcc caccatatcc ttgacgccgc
     1801 catacgcttc gtaggtccac agtgttcttc cggtggtttt gaggtagttg taacgttccc
     1861 gagatggctc cgtaaggact gatccaaaac tcaactggta aatgtcaacg acgtcaagca
     1921 cccttcgcag gtcggcaacc gtcataccct tgaaaggatc ggtgtacacc tggactcgcg
     1981 gatctattct tttgaacagc ctggcctggt attccagctc gtcaatgagt ttgccgccga
     2041 gcagtccagg ctcatctacc gggtagaaag cccagcgttc gtacccgaaa cctttctctg
     2101 caaggtgatt cacccagagg gggaggtact gcgaaaacgc gcgctcccat tccggagtgt
     2161 gggaaccagg tgcgtcgggc gccgggtgaa ggccacccgg actgatgagc atcatatcgc
     2221 tggggcgcag ccggtccaga atccagtcgt gctcggacca gtcgacttcg cctttgaggt
     2281 tgcccgcatt gtcgtactcc actttcggca accgcgtgat gacaaatacg ttggtgtgat
     2341 gcgcctgcat atcgcggatc gtagtttcct cgatatcgcg caacggggag gagtgggggt
     2401 agccccagtt gcagaaacga acgggcatct gttccggcaa ctggaaggag gaaaccgtcc
     2461 agaggatggg tatttcccac gtggtacccg caggggtaag cgccctcaaa tggagtgctg
     2521 tcgaatactc gccggcagga aggtcaccgg tgtcaacatc cacccataat cgtgccgagc
     2581 tggccgccgg caccccgata aggccagctt cgcccaggtc ggggagggcg tccggcccca
     2641 tttttgaagt tgatgtgggt acccaggcga ctctgcgcag agtgagatgg gcgaccgcat
     2701 caagtttctg gccggtgggg cacgtaatcc ggcccagaga cgcgcgaatc tccaggggca
     2761 cgtcgagcag gtttgcaatc tgcacgacga agacgtcgtg tccgcgcata tctgtcgtca
     2821 acgcaacccg ggaagcgcag tccccggcag gcacatcgaa ctccggcgcg aaggcgctcc
     2881 aggggttttc cacgatccac ggcaagaact cggtaggggc atctcgttcc gccgtcaacg
     2941 caactcgtct tttctgcagt tctgtatcgc ggcgaagttg gtcaaggaag tggcggattt
     3001 cttccacaaa tgcacggcgt tgctcactgg agcttttttc gtattggggc aaccgctcca
     3061 gaatatcttg ccacgtgagc ttccgggtgc gcgttccccg caccaggtcg ttcgtgatgc
     3121 cgtacccgcc ggcaatgcct gagaggtgct tcagcaggcg ctcaatttca gcctgttcat
     3181 cctggaaaag ggttacggag aactggctgg aagcggcgcc gatcggggca cgatcggcat
     3241 cgcgatatat cgttgcctgc accgtgtatt cgccgcaggt aaggatttcg aactcaacgc
     3301 cgcgttgcga atccgtgacg ccgaagtggg tgaccccatc agaaccccga agtgagacgc
     3361 tggcatacgt tccggacggg agatcgtaat caaggacgtt tgacccgagc cgcaatcgac
     3421 ccttcggtcc aaccacagcg ggaagatcgc ggggcgtctc aggttttctg gccagcggtc
     3481 tccccgtgcg gagattgttg gcgcgcccat cgaatgcgcc gcggcttgtc ggccactccg
     3541 ttttgcctcc gaggcttttg aaacaactca aggccccggt ggtggcgttt acggtgaaga
     3601 tttcgagcat cccgtcaccg tccaggtcgg cggcgacggg cgccccgatc atcgttccct
     3661 cgaggcggta attctcgatc tgcaccccct cggaatcaaa cacgcgaaga gtcgttccgg
     3721 cgcgttgaac gagcagcgat tccaacctgc cgtcgcggtc gacatctacc agggatggtg
     3781 caaatggttg gtcgccatct cctgaatccg tgtttgccca gatgagcgtg ccatccgacg
     3841 caaccgcctg gatcgcccgg ccggcgtgga caaatacgat ctccggaaca ccgtcggcgt
     3901 tgatgtcgcc ggctgcgatg ccgatttcga ggcttccggc tcctgcacca cgacaagtcc
     3961 acgcgacctc accctcaggg gtgatgcagc gaaggacgtc gtccatggac cctgccagta
     4021 cgcgtgggcc ttgcccgaaa tcaaccagta ccggtgcaga gaattcagcc ggtgcatcag
     4081 tcttgacgcg ccagaggagc cttccattca gatcgatgca atagatgaac gagtctcccg
     4141 cgcaaaagac gatttcatcc tcgccgtctc gatcgatatc acctattgcc gcgggtccca
     4201 cggctcgcac gttcgtgtgg aactgccacg catgctcccc ggaatcgagc gcaagaccgg
     4261 tgacacgtcc cttcgcgtcg gtcacgatga cgcgggagac gtgcccgccg aatgctattc
     4321 cgacgctgtt gaaggggctg gttgcggctt cgacgtcgga cgcccagacg agcgtaccgt
     4381 cgtctgaaag acggatcgcc ttccctgctt ccgtgacggc gagcacgcat ctgtcgggat
     4441 cattcgcaga cgatactgaa acccatgtcc ccaacttcga acctactggc gttctccagt
     4501 gctcgttgcg atctgaatcg atagcgacaa tgtgccccga attggttcca cagaccagga
     4561 gtgtgccgtc ctgcgtggga aggacggccg gacaacctac aaatccggca gtttcgaacg
     4621 tccacaaacg ggtgagcgaa ctcattgcgt tccctccggc ctgaggtttc accgtaaagc
     4681 cagaacatca aacatgtcgt acaacccttc gggtttgccc acaacccatt ttgcggcacg
     4741 gagtgccccc atcgcaaacg tatcgcggct ctgggcccgg tggcgcagtt ctatcgattc
     4801 gccgacgccc ccgaacaaga cgtcgtgctg gccgacgata tcccccgccc gcaacgagtg
     4861 cattccaatc tcatcaacag ggcgttcccc ggtgatgccg gttcttccgt ggcggacgga
     4921 tttttcaaaa tcccagcccc atgttttcgc taccgctttc gccagcgaga tggcggtccc
     4981 gctcggtgcg tctcgcttca atgtgtggtg ggcttccact atctcgcaat tgaaccgtgg
     5041 tccgagtaga tgcgccgctt cttcaaccag gcgcagaagc acggttacgc caacactgaa
     5101 attgcttgtc ttcacaaccg caatcccttg cgctgcgtct ttgaacgcct gttcctgctc
     5161 agacgtcagg cccgtagttc ccaggacaag cggctttgcg gcggctctgc agatggcggc
     5221 ctgctccacc gtggcttccg ggttattgat gaaggcaatg gcgacatctg cgcctttgat
     5281 ggcatctgcg aggttattga cgacgggaac cgagagcctc ccgacgcccg cgatttcacc
     5341 cgcatcgcaa ccgattgccg ggtcaccggg tttctccacg gctcccgtga tactgagttc
     5401 ggggtcttcg tgcgccaggg caacgatgcg ccgacccatt cgtcctgccg atccgcaaac
     5461 tgcgattcgt accatgaacc tcacgctcct gttatcgtgt tgcgccaggc cgcgggtccg
     5521 aagcggaccc gattcgtgca ttcctagtat agagccagac cggcaactgg aggaagtgct
     5581 ctgtttgctg cgaatccgcc ggttcgcgcc acacgatgcg ctaatggcat taatgtgcaa
     5641 tgattgcgca ctcgattgtt tttgggtcgc gccaacaagc atcgtgacgt cctttttatg
     5701 ggcatcaata atggaagtgc aacaatggca cactcattgc atactggaat tccgggttct
     5761 tcgaggcgca agggataacc ggagcacgga accccggaag c
//
LOCUS       DAOTNG010000201         5784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11773_length_5784_cov_4.883226, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000201 DAOTNG010000000
VERSION     DAOTNG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11773_length_5784_cov_4.883226"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="P5179_09890"
     CDS             <1..834
                     /locus_tag="P5179_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95569.1"
                     /translation="FTSGFLSKDMILGGAFAFGFVENPQHFLIPVFGFAAAVLTAFYM
                     ARLLIMTFLGTNRMAERGQEALRESPWNMTVPLVLLAVLCGWFWYNPDPTTIFAKGWV
                     SSFLTPPASAVPQTLVAAPADAHHAAHVAAMLVSLSCAGAGFFAAWMLGRRGAISLEH
                     WRLSGSRVYYLLANKYYVDELYNRTVVAGMVQVSRILGWFDKVLVDGVVNATGRFQTG
                     LSELSGFVDRYGVDGLVNALAGFVRDTGNEVRQIQTGRIQNYVVYATVGLIVLIVVTG
                     M"
     gene            848..2401
                     /locus_tag="P5179_09895"
     CDS             848..2401
                     /locus_tag="P5179_09895"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS95570.1"
                     /translation="MSNILSWLTFLPLVGMAAILVIPTRLRKSTGLIRYVALGTTLGC
                     FALAAWLFFAFDRSSSAMQFVENADWIPYFSIRYTLGVDGLSILMVLLTQIIGLVGVI
                     ASWNINKAVKGYFALYLLLQTGMTGVFCALDFVLFYVFWEVMLLPMYFLIGIWGGPRK
                     EYAAIKFFLYTLLGSVLMLLAILALYLNMQAPSFSITAMADPANFRADGWLVRNMPTW
                     FFGLTPKELVWLAFFVGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGILLKMGTYGI
                     LRVNFPILPAETVHFATFIAVIAVVNIVYGALCAMAQKDLKSLVAYSSISHMGYVMLG
                     MVSWRNEAGLNGAVLQMFNHGTITAMLFLLVGVVYDRAHHRRIDGFGGLWAKVPVFSS
                     LFVVASMAALGLPGLSGFISELLVFMGAFASYRGFAVIASSGIVLGAAYLLWTIQRVF
                     MGPLNQAYAEKDAMPEISPREIASVVPLMAIVIVLGVYPAPALNLIGPTLGSLLQTMQ
                     SAAGAAILP"
     gene            2421..3899
                     /locus_tag="P5179_09900"
     CDS             2421..3899
                     /locus_tag="P5179_09900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRS95571.1"
                     /translation="MGSIQDHIISSVHAFAPEGVLAATLLLIIVADLLPATSRPAVSL
                     GLALGGLAVSAVTVVRIPAGETLIFAGMLATDGFAATFKLVAAAVTAVVLVVSYRATD
                     VNREHFAEYCAMATALCLGAYVMAAAANLLSMFLAFELVSMSSYILTAHMKEDRKASE
                     AALKYVVFSASSSAVMLYGMSLIYGLTGTLVLKAIMPALAGNTASLAQSVGVLFVLAG
                     LTYKIAAVPYHFWCPDVYEGAPAPIAAFLSVGPKAAGMAMLARFLNEALFLPPGSPIR
                     SGIDFGNWQTAIVLISAVTMTLGNLSALRQTNVKRLLAYSSIAHAGYMLMALAVIRPF
                     GVTAMTFYVVAYALMNLGAFLVVIALGRSMRSEELDSYAGLGGRAPVVAVLMAIFLFS
                     LAGIPPTMGFVGKLFLFWAVLEGKGLAWLAVVAALNTVIALYYYARILRQMFFLPARE
                     GAGEIRVEPIHYVVLFLLAIPTLALGVYWGPLWGAISAAGTP"
     gene            3920..4543
                     /locus_tag="P5179_09905"
     CDS             3920..4543
                     /locus_tag="P5179_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95572.1"
                     /translation="MGHTPLAISLLVCVLSLQSAAAEHVTRTRWFFSFGPSAIFLSNA
                     AHDDLAGVSAPAGTGRGGSAQLGFEVTSHFAMRLAVHSARHSVAGPGTGGTASAAVDF
                     VGIQKYGAIAPFLFGRVGKQAFVLSNARTTTSTPPRDHSWNGHLLGGGTGVRLYLTRR
                     LSVAPEASLCWVRYNKVMSTDGTGTQLPLDPISGTTGSVSLLLSIHW"
     gene            complement(4578..5645)
                     /gene="arsB"
                     /locus_tag="P5179_09910"
     CDS             complement(4578..5645)
                     /gene="arsB"
                     /locus_tag="P5179_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRS95573.1"
                     /translation="MSAVATKRLSFLDRFLTLWIFLGMAAGVAVGYFVPGTEGFIHRF
                     QVGTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNGKILGLSLVQNWIIGPVLMFVLA
                     ITFLSGYPEYMVGLIMIGLARCIAMVIVWNDLARGDTEYCAGLVAFNSIFQVLFYSVF
                     AWFFITVLPPLFGLRGAVVDVGIGQIAESVFIYLGIPFIAGALTRLVLVKSRGKEWYH
                     GQFIPRISPITLIALLFTIVVMFSLKGNLIVQIPLDVVRIAIPLLIYFVIMFLISFWI
                     GKKAGADYAKTATLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLVEVPVLIGL
                     VNVSLYLKGRWFESPQGATSA"
BASE COUNT         1097 a         1696 c         1668 g         1323 t
ORIGIN      
        1 ttcaccagtg gttttctgag taaagacatg attctcggag gcgcgtttgc tttcggtttc
       61 gttgagaatc cacagcactt cctcattccg gtgttcgggt tcgccgccgc cgtactgact
      121 gcattctaca tggcgcgtct gctgatcatg acgttcctcg gcacaaaccg gatggcggaa
      181 cgtgggcaag aggcgctgcg ggaaagcccg tggaacatga cggttcccct tgtcctcctc
      241 gctgtgttgt gtggctggtt ctggtacaat ccggacccga caacgatttt cgcgaagggg
      301 tgggtgtcct ccttcctgac gccacccgct tcggctgtcc cgcaaacgct tgtagctgct
      361 cccgcagacg cccatcatgc cgcgcatgtg gcagcgatgc tcgtgtcact gtcctgtgcc
      421 ggagccggtt ttttcgccgc atggatgctc ggtcggcggg gcgcgatttc gttggagcac
      481 tggagactgt cgggatcgcg cgtgtactac cttctggcca acaagtatta cgttgatgag
      541 ctttacaaca ggaccgttgt cgccggaatg gttcaggttt cgcgcattct agggtggttt
      601 gacaaggttc tggtagacgg agttgtcaac gccacaggtc gcttccagac agggttgtcc
      661 gaactctccg gtttcgtcga ccggtacggt gttgacggcc tcgtgaacgc cttggccggt
      721 tttgtgcgcg atacgggcaa tgaagtgagg cagatccaga caggacgcat tcagaactac
      781 gtcgtgtacg caacggtggg gctcattgtg ttgatcgtag ttaccggcat gtgagggcac
      841 tccggatatg tccaacatcc tttcgtggtt gacatttctg ccgctggttg gaatggcggc
      901 gatcctcgtc attcctacga gattgcgcaa aagcacagga ttgatcaggt acgttgccct
      961 cggcacaacc ctggggtgtt ttgctctcgc ggcgtggctc ttcttcgcat ttgaccgcag
     1021 ttccagcgcg atgcaattcg tcgaaaatgc cgactggatt ccttacttct ccatccgtta
     1081 cacccttggc gtcgacggcc tgtcgatcct catggtgttg ctgacacaga tcatcggcct
     1141 cgttggcgtc atcgcgtcat ggaacatcaa caaagcagtg aagggctatt tcgcgctgta
     1201 ccttttgctc cagaccggca tgaccggggt attctgcgca ctcgacttcg ttctgttcta
     1261 tgtgttctgg gaagtcatgc tgttgccgat gtatttcctg atcggcatat ggggaggccc
     1321 ccggaaagaa tacgcggcaa tcaagttctt cctgtacacg ttgctgggaa gcgtattgat
     1381 gttactcgcg atccttgcgt tgtatctcaa catgcaggcg cccagcttca gcatcaccgc
     1441 gatggcagac cccgccaatt tccgggccga cggttggctt gtacgcaaca tgccaacctg
     1501 gttctttggc ctcaccccga aggaacttgt ctggcttgcc ttcttcgtgg gcttcgcgat
     1561 caaaatacct gcgttcccgt tccacacgtg gcttccagat gctcacgtgg aagcgccaac
     1621 agccataagc gtcattctcg ccggcatcct tctgaaaatg ggaacgtacg gcattctgcg
     1681 ggtcaatttc cccattcttc ctgcggaaac cgtgcatttt gcgaccttta tcgcggtaat
     1741 cgctgtggta aacatagtgt acggcgccct gtgcgcgatg gcacagaagg atctcaaaag
     1801 ccttgtggcg tattcctcga tcagccatat gggttacgta atgctgggca tggtttcctg
     1861 gcgtaacgaa gcgggattga acggcgccgt gctgcagatg ttcaaccacg gcacgatcac
     1921 cgccatgttg ttcctccttg tcggcgtggt gtacgaccgg gcacatcatc gccgtattga
     1981 tggtttcggc gggctctggg caaaggtccc cgtgttttca agcctgttcg ttgttgcttc
     2041 catggctgcc ctggggctgc ccgggctttc cggtttcatc agcgagctcc ttgtgttcat
     2101 gggtgccttc gccagctacc gcgggttcgc cgtaatagca tcatccggca tcgttctcgg
     2161 cgccgcgtac cttctctgga cgatccagag ggtcttcatg gggccactaa atcaggcata
     2221 cgcagagaag gacgcaatgc ccgaaatctc gccaagggag atcgcttccg tcgttccgtt
     2281 gatggcaatt gtgatcgtgc tgggcgtgta ccccgcgccg gcgctgaatc tcatcggccc
     2341 gacgctgggg tcgctcctcc agacaatgca aagcgccgct ggcgcggcga tcctccccta
     2401 ggccggaggg tcaggaagcg atgggttcga tccaggatca catcatatca agcgtgcacg
     2461 ctttcgcgcc agagggtgtc ctcgcggcaa cacttctcct catcattgtg gcggatctgt
     2521 tgccggccac ctcgcgcccg gctgtttccc tcgggctggc cctcggaggt ctcgcggtat
     2581 ctgcggttac cgtggtgcgc atacctgccg gggagacgct catcttcgcc ggcatgctgg
     2641 caacggatgg cttcgccgca acgttcaaac tggtcgcagc ggccgttact gccgtagtgc
     2701 tggttgtttc gtatcgcgca accgacgtca acagggagca cttcgcggaa tactgcgcaa
     2761 tggcgaccgc gttgtgtctg ggcgcgtacg tcatggcggc agccgcaaac ctgttgagca
     2821 tgttcctggc atttgagctg gtcagcatgt cgtcctacat actgaccgcg cacatgaaag
     2881 aagacaggaa agccagcgag gccgccctca aatacgtcgt cttcagcgca tcctccagcg
     2941 cagtcatgct gtacggcatg tcattgatat acggcctcac cggcacactc gtgctgaagg
     3001 caatcatgcc tgcccttgcg ggcaacactg cctcccttgc acagtctgtg ggcgtacttt
     3061 tcgttctggc aggcctcact tacaaaatcg ccgcggtccc gtatcatttc tggtgcccgg
     3121 acgtgtacga gggagcaccc gcgccgatcg cggccttcct ctccgtgggc ccaaaagcag
     3181 cggggatggc gatgcttgcc cgtttcttga acgaggctct cttccttccg ccgggttctc
     3241 ccattcggtc cggaatcgat tttggcaact ggcagaccgc aatcgtgctt atcagtgcgg
     3301 taaccatgac cctcggcaac ctttcggcgt tgcggcagac gaacgtcaaa cgccttctcg
     3361 cgtacagctc gatcgcccac gcagggtaca tgctgatggc gctggcagtc atcaggccgt
     3421 tcggcgttac cgcgatgacg ttttacgtcg ttgcgtacgc actcatgaac ctgggtgcct
     3481 tccttgtcgt tatcgcgctc ggacggagta tgcgcagcga agaacttgac tcgtacgccg
     3541 gtctgggagg tcgcgctcct gttgttgcgg tattgatggc gatctttctg ttttccctcg
     3601 ccgggatacc cccgaccatg ggatttgtcg gaaagctgtt tcttttctgg gcggttctgg
     3661 aagggaaagg gctcgcctgg ctggctgttg tcgcggcatt gaacacggta atcgcgctgt
     3721 actattacgc acggattctc cgtcaaatgt tcttcctccc cgcgcgagaa ggagctggag
     3781 agattcgcgt agagccgatc cattacgtcg tcttgttcct gctggcgatc ccgacgttgg
     3841 cgctcggtgt gtactggggc ccgctctggg gagcgatttc cgccgccggg acaccgtgaa
     3901 gcagcgggaa ggctccggaa tgggccatac tccactggca atctcgcttc tcgtctgcgt
     3961 cctctccctc caatctgctg cggcagagca tgttacccgc acacgatggt tcttttcctt
     4021 cgggccgtcc gcaatcttcc tctcgaatgc tgcgcacgat gatctcgcgg gggtttcggc
     4081 gccggcaggc acaggtcggg gtggctccgc ccagctcggg ttcgaggtga cttctcactt
     4141 cgctatgcgc cttgcagttc acagcgctcg gcacagcgtt gcgggacccg ggacaggagg
     4201 caccgcttct gcggcggtgg atttcgtagg cattcagaag tacggagcta ttgcgccatt
     4261 cctcttcggg agagtcggca aacaggcgtt cgttttgtcg aatgcccgga caactacctc
     4321 aacgcccccc cgtgatcatt cctggaacgg ccatcttctg ggcggaggca caggggtgcg
     4381 gctgtacttg acgcggcgcc tcagcgtcgc cccggaggcg tctctgtgct gggtgcggta
     4441 caacaaggtc atgagcaccg acggaacggg gacgcaattg ccgcttgatc ccattagcgg
     4501 cacgacggga tcagtctccc tcctgttgag tatccactgg taatcgaaac ttcatccctg
     4561 cggttcggga acacgcttca ggcgctggta gctccttgag gcgactcgaa ccatcgtccc
     4621 ttcagataca gggatacgtt gacgagacca atcagcaccg gaacctcgac gaggggaccg
     4681 atcaccgcgg cgaatgcggc gccgctgttg atgccgaaca cggcaactgc aacggcaatg
     4741 gcgagttcga agttgttgct cgctgccgtg aaactgagcg ttgcggtctt cgcgtaatct
     4801 gcccccgctt tctttccgat ccagaagctg atgagaaaca tgatcacgaa atagatcaac
     4861 agcggtattg ctatccgcac cacgtcgaga gggatctgga cgatcaggtt gcctttgagg
     4921 ctgaacatca ccacgatggt gaacagaagg gcgatcaacg taatgggact gattctcgga
     4981 atgaactgcc cgtggtacca ctccttccct ctggatttca ccagaacaag ccgcgtcagc
     5041 gcaccggcga tgaacggaat ccccaggtag atgaacacgc tctcggcaat ctgcccaatg
     5101 ccgacgtcga caacggctcc ccgaaggccg aacagcggcg gcagaaccgt gatgaagaac
     5161 cacgcgaaga cgctgtagaa gagcacctga aagatgctgt tgaacgccac gaggccggcg
     5221 cagtactcag tgtcacccct cgcaaggtca ttccagacaa tcaccatcgc gatgcaacgc
     5281 gccagaccta tcataatcag gccgaccatg tactccggat aaccagacag aaacgtgatc
     5341 gcgagcacaa acatgagcac gggaccaatg atccagttct gcacgagcga taacccgagg
     5401 atcttgccgt tgcggaacac atcgcccagc tcctcgtatt tcactttcgc cagcggggga
     5461 tacatcatca ggatgagccc gatggcgatc gggatgttgg tcgtaccaac ctggaaacga
     5521 tgaatgaatc cctcggtgcc aggaacaaag tagccaaccg ccacaccggc ggccatcccg
     5581 aggaaaatcc agagagtgag aaatctgtcg aggaacgaca ggcgcttggt tgcgacggcc
     5641 gacatagtgt tctcctttgg gactcacgga aaaccacaca gtacagtaat cgagtttcta
     5701 tttccagagg gacagagtag ccgaaatcag ggccgctcac tcgcagttgc cgccacagga
     5761 gcattgcagc gtgcgctccc ctcg
//
LOCUS       DAOTNG010000202         5759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11832_length_5759_cov_5.372721, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000202 DAOTNG010000000
VERSION     DAOTNG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11832_length_5759_cov_5.372721"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="P5179_09915"
     CDS             <1..312
                     /locus_tag="P5179_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95574.1"
                     /translation="WIEYGADMPYPPGTALYQISSAHATENWVVLRVLGDLIFTNTDN
                     NAVQTGYQVRQRFDIAFRLDTAKSDSTWQIASWSDRESLLNARIAVRPAENAHVLSPR
                     E"
     gene            409..1134
                     /gene="rsmI"
                     /locus_tag="P5179_09920"
     CDS             409..1134
                     /gene="rsmI"
                     /locus_tag="P5179_09920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRS95575.1"
                     /translation="MTGRLYLVGTPIGNLGDVSDRVRAVLASVSLIAAEDTRRTRILL
                     KELGVSCPLTSYHDHNERTKFPELLSALEEGKDVAVVSDAGMPVIADPGWHIIQAAIQ
                     RGIEIIPVPGPTAFVLALVVSGLPVHRFAFEGYLPKKSSARRRKLEELAEEPRTMIFY
                     ETPHSVRSTLRDALEVLGDRKASLSRELTKKFEETRRGTLSTLLSSCDDKVLRGEFVL
                     VVSGTESVETRTPRAECGSEHVQ"
     gene            1124..1750
                     /locus_tag="P5179_09925"
     CDS             1124..1750
                     /locus_tag="P5179_09925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRS95576.1"
                     /translation="MFSDLKLGFQVFVDPLVNHFIRRGYHPDTFTIMGLVFNTIAAGF
                     YAFGMFFEGALVMLFGSSTDMIDGQIARKTGGVSDGGALLDSVLDRYSEAVVLIALSF
                     HYARIGWMITSVLSAAALTGSLLVSYVRARAEGLNYECKVGLMQRPERVIFLAAASVF
                     GVFLNTPYQHVAAAVWVMAILTNLTTCERIVHVRRAAKQREMSLREQR"
     gene            1824..2579
                     /locus_tag="P5179_09930"
     CDS             1824..2579
                     /locus_tag="P5179_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRS95577.1"
                     /translation="MRGAAAFCRLHYSVGNAAAALGYREIMPLIQSALADAGLLARNP
                     DGTTPKLRFGPPLPAGCSSMREYCDAELDSTLDDVLSRINSRLADGLQCVQCDRIDRK
                     AEPVDRIAFYAEYSFEIPHSLLRECDVKALRELFDEQGEWLAERVSPKGHVRESDLKK
                     AVTNWRSESSASITTVYLRLAIAHEEGHTASPALVLQKLFDLNEEQSALVAITRREMF
                     DRSGAPLRSRPWPRKRNRLLHERCWEHQRFHHQ"
     gene            complement(2590..3531)
                     /locus_tag="P5179_09935"
     CDS             complement(2590..3531)
                     /locus_tag="P5179_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95578.1"
                     /translation="MLFLRIRLCPSIRNVEPDRWDYDSLRDALRLIGRWYRFVSMDEA
                     VSALAGRSPLFKSSIAVTVSIGSSLPEIDLGRLAIECDVPLTVFFCPGAVEAGVHPWY
                     CVLAFALNGLDGQTVTVGGRRWLLSNYDYRQLAFADIARQIASLPLTDRYRYTEELVS
                     GWRAKPAGSEVITFREMMRIMRSKRITFGVSGYSGDSLMRLPVERALYEIREGFALYR
                     RVFGEEPAHFEYPWGDRSVYLEEFVRNSGYRSGTLGLNAMDGLNRIGADPFRLAAQNL
                     RPRTPARIRADLAGVSKQLRPLERFLDVRTRRADRDF"
     gene            complement(3669..4502)
                     /locus_tag="P5179_09940"
     CDS             complement(3669..4502)
                     /locus_tag="P5179_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRS95579.1"
                     /translation="MLNSPLPATVPVDEAARVTEQAAVAPGFFRLRFHAPHIARHAVP
                     GQFVQVRVTDDLEPFLRRPFSIGDADPSRGLIEIVYHVVGKGSRRLSERKAGETIPLL
                     GPLGNGFRLPGRGTVVLVGGGVGMPPVYFAAKSIRGCHVCVIQGARCAERLLYREELS
                     SLGIPHRETTEDGSAGSQGLVTAALIETLSDFPPEERKGHVEILACGPPPMLAAVAEI
                     ARAHGVRCQVSVEERMACGFGICMGCAVQKRTAGSKEYALVCVDGPVFDATEIALAPS
                     S"
     gene            complement(4492..>5759)
                     /locus_tag="P5179_09945"
     CDS             complement(4492..>5759)
                     /locus_tag="P5179_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95580.1"
                     /translation="FTDQVPRTRLEIARTKEAAGEIEAAVLLYQKVLEDAERLSPPTV
                     TQAASPGSGPAGGAAPTGVRTRETAEANFHLGRLQEQRFRNFANAAGHYALAAQSPDQ
                     EVALPAKRRMDDISLWTQLHATLADTTDSVSVSRKDRARYSLAEHHLLAMDEPDSALF
                     YFDAVAERTSDSDLRMRAQYASGWIWREVKRDTARSDSIWQKLITDSSRTRSTMEIKR
                     AIHSVQCRPEQEPGIDLYQEAEGRWFDSLVRCPPYPPDSVTKDSLSSATWWDEWRACE
                     KRECESAIKDFNRLLELYPQGNLATRARFILGWYAENVLEDTASAYRWYDAAREDSVF
                     APEVGRVADLLLKSRVTGGTGRSAETAGIRDAAAADTTAEVRQPLEPRTPREPEPEAP
                     PQPEERSLERPERVERVPVPRREPRRHAE"
BASE COUNT         1181 a         1752 c         1635 g         1191 t
ORIGIN      
        1 tggatcgagt atggagccga catgccatac ccgccgggaa ctgcgcttta ccagatttcc
       61 agcgcgcacg ctactgagaa ctgggtcgta ttgcgcgttc tcggggacct gattttcacg
      121 aacactgata acaacgcagt tcagacgggc taccaggttc gtcagcggtt cgacatcgca
      181 ttcagactcg ataccgcgaa gagcgattcc acgtggcaaa tcgcatcgtg gtcagaccgt
      241 gagtccctcc tcaatgccag gatagcggta cgtcctgccg aaaatgccca cgttctgagc
      301 cctcgagagt aatcgggttg ccagcgtacg cgccggatga gtatcttttt cagagtgcgc
      361 gggggcgttt tttctgctta ttcttcgctg tgagaaggtg atccggttat gaccggtcgg
      421 ctttaccttg tgggaacacc gatcgggaac ctcggggatg tgagtgaccg ggttcgcgcg
      481 gttcttgcga gcgtttccct tatcgctgcg gaagacacgc gccgaacgcg tattctgctg
      541 aaagaactgg gcgtttcgtg ccctctcacg agctatcacg accacaacga acgaaccaaa
      601 ttcccagaac tcctttcggc gctggaagaa ggtaaggacg tcgccgtggt cagcgatgcg
      661 gggatgccgg ttatcgcgga cccgggatgg catatcattc aggcagccat tcaacgggga
      721 attgaaatca tcccggtacc cgggccaacc gcattcgtcc tcgcactggt agtctcaggt
      781 cttcccgtcc accgtttcgc gtttgaaggg tatcttccca agaaatcgag tgcgcggcgc
      841 cgaaaactcg aggagctggc ggaggaaccg aggacgatga ttttttacga gacgccccat
      901 tccgtccgca gcactcttcg cgatgccctt gaagtgcttg gagacaggaa agcgtcattg
      961 tcgagggaat tgaccaagaa attcgaagag acgcggcgag gtactctttc aacgcttctt
     1021 tcatcctgcg acgataaggt gttgcgcggc gagtttgtac tggtggtgtc gggcacggag
     1081 agtgtggaga cacgcacgcc ccgtgcggaa tgcggatcag aacatgttca gtgacctgaa
     1141 gctcgggttt caagttttcg tcgatcccct cgtgaaccat ttcattcgtc gggggtatca
     1201 cccggatacc ttcacaatca tgggtctggt gttcaacacg atcgcggccg ggttctatgc
     1261 cttcgggatg ttcttcgaag gcgctcttgt gatgctgttc ggtagctcca cggacatgat
     1321 tgacgggcag attgcccgca aaactggcgg agtatccgac ggtggagcgc tgctcgattc
     1381 tgttctcgac agatacagcg aggccgtggt cctcatcgcg ttgtccttcc attacgcacg
     1441 aatcggctgg atgataacct cggtgctgag cgcggcggcg ctgaccggtt ctcttctcgt
     1501 cagctacgtt cgggcgcgcg cggaagggct gaactacgaa tgcaaagtcg gtttgatgca
     1561 gagacctgaa cgtgtgattt ttctcgccgc cgcatcggtt ttcggcgtgt ttctcaatac
     1621 tccctatcag cacgttgccg ccgcggtatg ggtaatggcg atactcacga acctcaccac
     1681 atgcgaacgc atcgtgcacg tgcgaagggc cgcaaaacag cgggaaatga gcctgcgcga
     1741 acaacgttga cgactgagca acacgccccg aaggacactt cgaactggaa tccacccgac
     1801 tcctcgacgg ctgcacgccg ccggtgcgcg gagccgcggc gttttgccga ctccattact
     1861 ccgtcggaaa cgcggccgca gctctcggat atcgggagat catgccactc atacagtcgg
     1921 cgctcgccga tgccggcctg cttgcacgaa accccgacgg aaccacgcca aaactgcggt
     1981 tcggcccgcc acttcccgcc ggttgttcca gcatgcggga atattgcgac gcagaactcg
     2041 acagcactct ggacgacgtg ttatcgcgaa tcaattcccg tctcgctgac ggcctgcagt
     2101 gtgtccagtg cgaccggata gacaggaaag ccgaacctgt ggaccggatc gcgttctacg
     2161 cggaatactc gttcgagata ccacactccc tgcttcgcga gtgcgacgtg aaggctttgc
     2221 gcgagctgtt cgatgagcaa ggagaatggc tcgcagaaag ggtcagtccc aaagggcacg
     2281 tccgggaaag cgatcttaag aaagctgtta ccaactggcg gtccgaaagt tccgccagca
     2341 tcacaaccgt ctatcttcgg ctggcgatcg cgcacgaaga ggggcacacg gcctctcccg
     2401 ctctcgtact tcagaagctg tttgacctga acgaggagca atcggctctg gtcgcaatca
     2461 caaggaggga gatgttcgac cgcagcgggg ctccattgag aagcagaccg tggccgcgga
     2521 aacgtaatcg gttgttgcac gaacgctgct gggaacacca gcgtttccat catcaatgac
     2581 caggattgtt cagaagtcgc gatccgctct tcgcgttcga acgtcgagga atctttccaa
     2641 cgggcgtaac tgcttgctca cccccgcgag gtccgcacgt atgcgagcag gcgttcgcgg
     2701 cctcagattt tgtgccgcca gccggaacgg atccgctcca atacggttca gcccgtccat
     2761 ggcattgagg cccagggtac ccgagcggta cccggaattc cttacgaatt cctccaggta
     2821 gacgcttctg tctccccatg ggtactcaaa atgggctggt tcctccccga aaaccctccg
     2881 gtaaagagca aacccttcgc gtatctcgta cagggcgcgt tccacgggca gacgcatgag
     2941 cgaatctcca gaataccccg aaaccccgaa ggtaattctt ttgctacgca ttattctcat
     3001 catttcacga aacgtaatta cctcgctacc tgcgggcttt gcgcgccagc cggaaaccag
     3061 ctcttccgtg taccggtagc ggtccgtcaa cggcaaactc gcaatctgtc gcgcgatgtc
     3121 cgcgaaggcg agctgtcggt aatcgtagtt ggaaagaagc cacctcctgc caccgaccgt
     3181 aaccgtctgg ccgtcaagcc cattcagggc gaacgccagc acacagtacc acgggtgtac
     3241 gccagcttcc actgcgccgg gacagaaaaa cacggtgaga ggtacgtcac attcaatggc
     3301 gagccgcccc agatctattt ccggcaggct gctcccgatc gaaacggtaa ccgcaatgct
     3361 gcttttgaag agtggggacc tgcctgctag tgccgacact gcctcgtcca ttgagacgaa
     3421 acggtaccag cgtcctatga gacgtagagc atcccggaga gagtcgtaat cccatcggtc
     3481 tggctccaca ttccgaattg atggacagag gcggatcctc aaaaacaaca cgccggcatt
     3541 gcgccttttc cactcaacga accgggctac acctgtgctc gaacaaaggc gatacagggc
     3601 ttcggagggc ttttctgccg ggagaggaat cggaacacga ggcttctttt tcgcgggacc
     3661 tgttcgattc atgacgaagg agccaatgcg atctctgttg cgtcgaacac cggaccatca
     3721 acacacacaa gcgcgtattc tttgctgcct gccgtgcgct tctgcacggc acatcccatg
     3781 cagatgccga acccgcacgc cattcgctct tccaccgata cctggcacct caccccgtgc
     3841 gcgcgcgcaa tttcggctac cgcggccagc attggaggtg gtccgcacgc gagtatctcg
     3901 acatgccctt tgcgttcctc gggcggaaaa tcggagagcg tttcgatcaa cgctgcggtg
     3961 acaaggccct gtgaccctgc cgacccatcc tccgttgtct cgcggtgcgg gataccgaga
     4021 gacgagagtt cctcccggta cagcaatcgc tccgcgcacc gcgccccttg aatgacgcac
     4081 acgtggcatc cgcggattga cttcgcagcg aaatataccg gcggcatgcc aacgccgccg
     4141 cccaccagaa caacggtgcc gcggcccggc agcctgaatc cattccccag cggaccaagc
     4201 agcgggatcg tttcgcccgc cttccgttcg gagagtcgcc tactcccctt tcccacaacg
     4261 tgatacacga tttcgatcaa gccgcgtgag gggtcagcgt ccccaatact gaacggacgg
     4321 cggagaaagg gctcgaggtc atccgttact cgaacctgaa cgaactgccc tggaacggcg
     4381 tgtctcgcaa tgtgcggtgc gtggaaacgc aaccggaaaa agccgggagc gacggctgcc
     4441 tgttcggtta cgcgcgcagc ttcgtccaca ggcacggttg caggcaatgg gctattcagc
     4501 atggcggcgg ggctccctcc gtggaaccgg cacgcgttcc actcgttccg gccgctccag
     4561 agaacgctcc tccggttgtg gaggtgcttc tggctccggt tcccggggtg tacgcggctc
     4621 caatggttgt ctgacttcgg ctgttgtgtc agcggctgcc gcatctcgaa ttcctgcggt
     4681 ctctgctgaa cgccccgttc caccggttac gcgtgatttc aaaaggaggt cagcaacacg
     4741 cccgacttcc ggcgcgaaca cggaatcttc gcgcgcagca tcgtaccaac ggtacgcgga
     4801 agcagtgtcc tcaaggacat tttccgcgta ccaaccgagg atgaaccgtg cgcgagtcgc
     4861 cagatttccc tgcggataca gctccagaag gcgattgaag tccttgatcg ccgattcaca
     4921 ctctcgtttc tcgcacgctc gccactcatc ccaccatgtt gcggaggaca gagaatcttt
     4981 cgttaccgaa tccggcgggt acggcgggca acggaccagg gaatcgaacc agcgcccttc
     5041 ggcttcctga tacaggtcga tcccaggttc ctgctcgggg cggcactgaa ctgagtgaat
     5101 ggcccttttg atttccatcg tgcttctcgt ccgcgacgaa tcggtaatca atttctgcca
     5161 gatcgaatct gaacgggcgg tatcccgttt cacctcccgc cagatccacc cggaggcgta
     5221 ctgcgcgcgc atccggagat ccgaatcact cgttcgctcg gcaaccgcgt cgaagtaaaa
     5281 cagcgcggaa tccggttcgt ccatcgccag caggtgatgt tccgcaaggg agtacctcgc
     5341 ccggtctttc ctggagacgc tcacagaatc agtggtatca gcaagtgttg cgtgtaactg
     5401 cgtccagaga gatatgtcgt ccattcgccg tttggcgggc agggcaactt cctgatccgg
     5461 actctgcgcc gccagggcat agtgccctgc agcgttcgcg aaattgcgaa atctctgctc
     5521 ctgcaaacgt cccagatgga aattcgcctc cgccgtttcc cgcgttctga cacccgtcgg
     5581 tgccgccccg ccggccggac cgctgccggg ggaagcggct tgagttactg tcggaggact
     5641 gagacgttcc gcatcttcga gaactttctg gtacagaaga acagcagctt cgatttcgcc
     5701 cgccgcttct ttcgtcctgg cgatctccag tctcgtacgc ggcacctgat cggtgaacc
//
LOCUS       DAOTNG010000203         5737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11878_length_5737_cov_5.456705, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000203 DAOTNG010000000
VERSION     DAOTNG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11878_length_5737_cov_5.456705"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5179_09950"
     CDS             <1..573
                     /locus_tag="P5179_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95581.1"
                     /translation="DTGEAQVLFKTDYLMHLHLHDEGEGRLLATVRPPDLEWGIYTFD
                     LDGGDFQKLPFTRSTNHFASLGCTGKVITTVHGGGNAIEIGTPGGASAEVLVEGEGFW
                     HPTADSTGEWVACDTNWPDTGLMLVHVPTRRYRVLCFTGTSGGHPQWAHAHPRMSPDA
                     SYVVFDSDKSGITQTYAVFVPPEFKEELRG"
     gene            622..1659
                     /locus_tag="P5179_09955"
     CDS             622..1659
                     /locus_tag="P5179_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95582.1"
                     /translation="MGCERVARTDPETGVRILQLTSFPITHWHLGLGDFITPDGERLL
                     IAANRFAGRNSPRDLYSIRADGDDFNFLLHDFSGGVICPRVQWVYTGCAGGVVRIPIE
                     GGTAEVLWSPDCYTHIMVSARSSDGLWLFGQGRRKTDGSFDIVRISTETGEASVIVRA
                     SYVMPVLRSSANGERLVASIAPVGDDGKPLMPWGVWEFSSEGTDFRLVPFSRSTNHYC
                     PMGSTGLVVTTAAHPGNALDVAAPGGCERVLAHGAGFWHVSSDETGTWLASDTNWPDV
                     GLQIVNSHTGKYATLCMTGASGGHPQWTHAHPVISPDARWVLFTSDRTGFQHVYLADI
                     PPEFKKALSAA"
     gene            1969..3339
                     /locus_tag="P5179_09960"
     CDS             1969..3339
                     /locus_tag="P5179_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS95583.1"
                     /translation="MPSNLRLLLADGDRSSLTIMQEITAQWGHQVLACRSVAEAVSAM
                     DAHKVDVVVTDLVLPDGSGLDLVRHAHEIDVPVASIVISAHGTIAGAVDAVRLGAYDF
                     LTRPVDLAKLKVIIPAAFDKERLRFENRQLRSKISEETRPEGLVGQSLAIRQVIEKIR
                     MAANARATVLITGESGTGKEVIATAIHKLSPRSAEPLVKVATGALAPQLLESELFGHE
                     RGAFTGAVAQRRGRFELAHTGTLFLDEIDGLEIGLQGKLLRVLQERQFERVGGEQTIT
                     VDVRLIAATNANLEKMVEQGLFRKDLYYRLNVVRIEAPALRDRREDIPLLAYHFVALY
                     AQENGKTIRGIDPAAMALLQSYEWQGNVRELENAIESAVVMCPGDWITADLLPQNVRS
                     GPPEEMQFRVGMTLEEMERRAIEATLRATHGNKTAAAKMLGIGVRTIHDKIKKLRPAG
                     ASEPTE"
     gene            3415..3804
                     /locus_tag="P5179_09965"
     CDS             3415..3804
                     /locus_tag="P5179_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95584.1"
                     /translation="MGPASASEASGHTTQPEWFPAVVELLRVRGYLTLSVKIGYELVL
                     IVSGSAGEAAVIDLSQEPTSLWTDAWAGMRQVLPRVPVIAILPAGNTDETIMPEHGAV
                     ARVVSPVDAESLVGIVEHVLQNPSYSR"
     gene            complement(3855..5051)
                     /locus_tag="P5179_09970"
     CDS             complement(3855..5051)
                     /locus_tag="P5179_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS95585.1"
                     /translation="MPKVLSSRLQRLPPYLFARINGMRLQLRQQGMDIIDLAMGNPDR
                     PTPKHIVDKLIEVSQDGKAHRYSASRGIPALLKAIARYYDRKYGVSLDWEKEVVATIG
                     SKEGIGHLVLALIDPGDLALVPNPAYPIHIYSVALAGGNVVTVPLHEEQDFVPSMAEI
                     TREIWPKPRIITLNFPNNPTTATVSLDFFQEVVDYAKKNDIIVIHDMAYSEITFDGYE
                     SPSFLQAKGAKDVGVEFFTLSKSYNMAGWRIGACVGNADVVGALAKIKSYYDYGIFTP
                     VQVAGIAALDGPQDCVREAAAVYQARRDVLCEGLNRIGWLVKKPKASMFVWAPIPEPY
                     RHMGSMDFSLMLMEKAEVAVAPGIGFGDLGDDYVRIALVENEHRLRQAIRNIRRSLTE
                     LKPADT"
     gene            5198..>5737
                     /locus_tag="P5179_09975"
     CDS             5198..>5737
                     /locus_tag="P5179_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95586.1"
                     /translation="MKRLNNMVVELARGVPKASDEFFECEFESPRSGWHYVGCSWDGE
                     SGDVRFVVDGRAAGTRPAFGSLVEAMLQLPAGPHTLRVEKTADGRLGEVCVRAVPELA
                     YCKHQYNPHIPEYGPYDWDFIARTVLPHVNTVVGNGDPSHEEHLKWWKARGGKWIVET
                     SLHGMNDETVTADDVWRNWT"
BASE COUNT         1218 a         1583 c         1750 g         1186 t
ORIGIN      
        1 gacacggggg aggcgcaagt tcttttcaag acggattacc tcatgcacct tcacctgcac
       61 gacgaaggtg agggtcggct tctcgccacc gtccgtcctc ccgatttgga atggggaata
      121 tacacattcg atctggatgg gggagatttc cagaaacttc cgttcacgcg gagtacaaac
      181 catttcgcat cgctcggttg caccgggaaa gtgattacta cggttcacgg cggcggcaac
      241 gccatcgaga tcggtacccc tggcggcgcc tctgccgaag tgttggtgga gggcgaaggg
      301 ttctggcacc cgacggctga ctcaacgggc gaatgggttg cgtgcgacac aaactggccg
      361 gatacgggct tgatgctggt gcatgtcccg actcggcgct accgcgttct ctgcttcacc
      421 gggacgagtg gcggccaccc gcaatgggca catgcgcacc cgcgaatgtc acccgacgcg
      481 agttacgtcg tgttcgacag cgacaaatcg gggatcactc agacgtacgc agtcttcgtt
      541 cctcccgaat tcaaagagga actccgaggc tgaccgaacc ctatcgcagc gtttcttcct
      601 cattgcagcg gggtattgtc catgggttgt gagcgcgtgg cccgaaccga ccccgaaacc
      661 ggcgtcagga ttctccagct gacgagcttt cccatcactc actggcatct cggccttgga
      721 gacttcatca cgccggatgg tgagaggctc ctgattgcgg caaacaggtt cgcgggaagg
      781 aactcgccgc gagacctgta cagtattcga gccgatggag atgattttaa cttcctgctg
      841 cacgattttt ccggtggtgt catctgcccg cgggtgcaat gggtttacac cggctgtgcg
      901 ggcggcgttg tgcggattcc gatcgaaggc ggaacggcgg aagttctctg gagccccgat
      961 tgctacacgc atatcatggt ttccgcgcgg agcagcgacg ggctgtggct gttcgggcag
     1021 ggccggcgca aaaccgatgg cagcttcgac attgtcagga tttcgacgga aaccggggaa
     1081 gcctctgtca tagtgcgggc gagttacgtc atgccggttt tgcgcagcag cgcaaacggg
     1141 gaacgactgg ttgcgtcgat cgcacccgtc ggagacgacg gcaagcctct gatgccgtgg
     1201 ggcgtgtggg agttctcgtc tgaaggcacc gatttccgcc ttgttccatt ctcgcggagc
     1261 acgaaccact actgcccgat ggggagtacc ggcctggtcg tcacgaccgc cgcgcatccg
     1321 ggcaatgccc ttgatgttgc agcccccggc ggatgtgaac gtgttctcgc gcacggtgcg
     1381 gggttctggc atgtcagttc tgacgaaacc ggcacttggc tcgccagtga tacaaactgg
     1441 cctgatgtcg ggttgcagat cgtgaactcg cacacgggga agtatgccac actctgcatg
     1501 acaggcgcca gtggcggaca tccccaatgg acacacgcgc acccggtgat ctcaccggat
     1561 gcgcgctggg tgctgttcac gagtgaccga acgggcttcc agcacgtgta tctcgccgac
     1621 attcctcccg agttcaaaaa agcgctgagc gccgcatgat acgagccttc cacgggcgcg
     1681 cgaaaggttc gtgaggccgc atttgaaaca gggtaagcgg ctaacacgcg gatgcagtcc
     1741 gcgcaacaag cgcctgcgac agtttttccc ggcccggatt gacgagagtc tgcaaaactc
     1801 gctacattgt agtgtccatt gctgcgcatt tgtgcaaaat tcgccggcta cacgaacgaa
     1861 tgataggtcg taatcgccgg ttggcgcttc tgtattgcac ggaatcatcc tgatctcatc
     1921 gcagatggga caaaaacttc gcgttcctgc aaacccgggg tcgtgttcat gccttcaaac
     1981 ctgcgtctgc tcctggcgga cggagatcgc agttccctga cgatcatgca ggagattacg
     2041 gcccagtggg ggcaccaggt tcttgcctgc cggagcgtgg cggaagccgt ttccgccatg
     2101 gacgcccaca aggttgatgt ggtggttacc gacctggtcc tcccggatgg cagtggactg
     2161 gacctcgtca gacacgcaca cgaaatcgac gttccggtcg cctctattgt gatttccgca
     2221 cacggaacga ttgcaggagc agtggatgcc gtacgtctgg gggcatacga tttcctgacg
     2281 cgcccggtgg atctcgccaa gctcaaagtg atcattcccg cggcgttcga caaagaacgc
     2341 ctccggttcg aaaatcggca gctccgttca aaaatcagcg aggaaacgcg gccggagggg
     2401 ctggtaggcc agagtctggc gatcagacag gttatcgaaa aaatccggat ggcagcaaat
     2461 gcccgtgcta cggttctgat taccggtgaa agcggtacgg gaaaagaggt tatcgccact
     2521 gcgattcaca aattgagtcc gcggagcgca gagccgctgg tgaaggttgc cacaggcgct
     2581 ctggctccgc agctgctgga aagcgaattg tttggccatg agcgcggcgc tttcacgggt
     2641 gcggtggcgc agcgtcgcgg gcggttcgaa cttgcgcata cgggaactct gttccttgac
     2701 gaaatagatg gtctggaaat cggcttacag ggcaaattgc tcagggtact ccaggaacga
     2761 cagtttgagc gggttggcgg cgagcagacg attacggtgg acgtccgcct tatcgccgcg
     2821 acgaacgcca atctggaaaa aatggtcgaa caggggctgt tccgaaagga tctctattac
     2881 cggctgaatg tcgtcagaat agaagcaccg gcgttgcggg accggcgaga ggatatccct
     2941 ctgctcgcct accactttgt cgcgctctac gcgcaggaga atggaaaaac aatccgcgga
     3001 attgaccctg ccgccatggc attactccag agctacgaat ggcaggggaa tgtccgggaa
     3061 ctcgaaaatg ctatcgagag cgcggtggtg atgtgtccgg gagattggat tactgcggat
     3121 ctcctgccgc agaatgtgcg cagtggaccg ccagaggaga tgcaattccg tgtaggtatg
     3181 accctcgagg aaatggaacg tcgtgcgatt gaagcgacct tgagggcgac tcatggtaac
     3241 aagactgctg cagcgaagat gctgggtatc ggggtcagga cgatccatga caaaatcaaa
     3301 aagcttcgcc ccgccggagc gtcggaacca acagaatgaa cttgcgacgg tacggagatt
     3361 gcatcacgtg acttcgtccg gacactcgga atcgcgagca tgaggagcaa ctgaatggga
     3421 cccgcgagcg catcggaagc ttccggtcac actacgcaac cggaatggtt ccccgctgtc
     3481 gtagagcttc tgcgcgtccg aggctacctt acgctttccg tgaagatcgg ctacgaactg
     3541 gtgctgatag tcagcggatc ggctggcgaa gcagcggtca ttgacctctc acaggaaccg
     3601 acctcactgt ggacagatgc ctgggcaggc atgcggcagg tgctgcccag ggttcccgtg
     3661 attgcgattc tgccggcggg gaacacagac gaaaccatca tgcctgagca cggtgccgtt
     3721 gcgcgtgttg tgtcacctgt agacgcagag agccttgtgg gtatcgtgga gcacgtcctg
     3781 cagaacccct catattcacg ttgaccacgc cttcgctcct gccggtttgt ccacaccacc
     3841 aagctgacca ctgtttacgt atccgcaggc ttgagctccg tgagcgaccg ccgaatgttg
     3901 cggatcgcct gcctcaggcg atgttcgttt tctacgagtg cgatgcggac atagtcgtcg
     3961 ccaaggtcgc cgaacccgat gccgggagcc accgcgacct ccgccttttc catgagcatc
     4021 agactgaaat ccatactccc catgtgccgg tacggttccg ggatgggtgc ccagacgaac
     4081 atcgatgcct tgggtttctt caccagccat ccaatgcggt tcagcccctc acacagtaca
     4141 tcccgccggg cctgatacac agcagcggct tcacgtacgc agtcctgtgg cccgtccagt
     4201 gccgcaatcc cagccacctg gaccggggtg aaaatgccgt agtcgtagta gctttttatt
     4261 tttgcgagcg ctccgaccac gtcagcattg cccacgcacg ctccgattct ccaacctgcc
     4321 atgttgtagg atttggaaag ggtgaaaaac tccaccccga cgtccttcgc ccccttcgcc
     4381 tgaaggaaag aggggctctc gtatccatcg aacgtgattt cggaatatgc catatcgtgg
     4441 atgacgatga tatcgttttt ctttgcgtaa tcgacgactt cctggaaaaa atcgaggctt
     4501 acggtagccg tggtaggatt gttcgggaag ttcaacgtga tgatgcgcgg tttcggccag
     4561 atttcgcgcg tgatttcggc catgctgggg acaaaatcct gttcctcgtg aaggggaacg
     4621 gttaccacat ttccccccgc gagcgcgacg ctgtaaatgt gtatcgggta cgcggggttc
     4681 ggaacaagcg ccaggtcccc cgggtctatg agggccagaa caaggtgtcc tattccttct
     4741 ttcgagccga tggtggcgac gacctccttt tcccagtcca agcttacacc gtactttcga
     4801 tcgtagtacc gggcgattgc tttgagaagc gcaggaatcc cgcgtgaggc cgaatagcgg
     4861 tgggctttac catcctgaga gacttcgatg agcttgtcca caatgtgttt tggtgtgggc
     4921 cggtcaggat tgcccatcgc aaggtctatg atatccatgc cctgctgacg gagctgaagt
     4981 cgcatcccgt tgatgcgtgc gaaaaggtac ggtggcaggc gctgcaggcg actggaaagg
     5041 actttgggca tctgcttcct ccagatggta aacgtgtaag gaacgatttt caaagatagc
     5101 aaaccgacgg cgaagaatac agcgcatcac ccgaaaaagc gcgttcggag cgatggtctg
     5161 gacatctcct tttcacctgt tcgtgaagga cgactgaatg aaacgcctca ataatatggt
     5221 tgtggaactc gctcgcggtg ttccgaaagc cagtgatgag ttctttgaat gtgaattcga
     5281 gtccccccgg tccggctggc attacgtcgg ctgctcatgg gacggggaaa gcggagacgt
     5341 caggttcgtc gttgatgggc gtgcggcggg tacgcgcccc gcgttcgggt cgctcgtgga
     5401 ggcgatgctt cagttgccgg cagggccgca cacattgaga gtggaaaaga cagcagatgg
     5461 acggctgggc gaagtgtgcg tcagggcggt gcctgagctg gcgtactgca aacaccagta
     5521 caacccccac atacctgagt acggcccgta cgactgggat ttcatcgcgc gaaccgtact
     5581 tccccatgtg aacacggtag tgggaaacgg ggacccaagc catgaagagc atctgaagtg
     5641 gtggaaagcc aggggaggaa aatggatcgt agaaaccagc ctccacggga tgaacgacga
     5701 aaccgttaca gcagatgacg tatggcggaa ctggaca
//
LOCUS       DAOTNG010000204         5736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11881_length_5736_cov_4.968844, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000204 DAOTNG010000000
VERSION     DAOTNG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11881_length_5736_cov_4.968844"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2582
                     /locus_tag="P5179_09980"
     CDS             <1..2582
                     /locus_tag="P5179_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRS95587.1"
                     /translation="NEVLARARVTNNPDSSQFSPSQRLTTSAAIDLIHRAGGCAFVAH
                     PLQTEPDPVRLRSLLEELRGSGLDGVEAFYGPLLPDKREAVAQLAQSAGLLVCGGSDG
                     HGNGEAGMGIDMPLLRWKEFRNALIEGAAQKTHRGPSPQPARPVEERFHWRAFLFHIA
                     MPAFLAVTLFVVALFVLLLPYFERTLMDRKRENIRELTQTAWSVLDEAEREASAGNLT
                     REQAQALAIKRVEAMRYGPEGKDYFWLQDLTPRIIMHPYRRDLNGHDVSQFRDPNGIR
                     IFVEFADLVRKRGEGYVSYVWQWKDDPERMEPKESYIRLFAPWGWVIGTGIYVHDVQR
                     EIAGLRQRLVNVSLGVTGAVLLLLLYIVRQSVKLERARRDAERRLRESTDRYRALTSA
                     ATEGALFVVGRRCAYANPVMSEITGYTQTQLELVDVDDLFPAVEANIEWRRRLDNPGG
                     ADTDIPVPGVLQRRDGTRLACSLSLRRVPESPRGYMILARNVTAASEPPEARALLNRL
                     LLLPEGVASDLTAAIQHATKPADVVGACLRAPKLVRALLENGASPMLVTRVLAELTDE
                     ATRRLIFLATEDIGPAPCPYSFLALGSQGRQSQTLYTDQDNALLYSLTDSEDPETANR
                     WFLELGQRVCTDLARAGFRDCRGGSMASNPRWCQPLPVWKRYFEEWIRNAEPREVMEF
                     SIFFDFRSVDGDVELPRELRRHVTGVLRTAPHFFAQAAQNALQFKPPLRLFGSIVGGR
                     DREHSGLLDLKSVMMPIVSFARLYALQQGVEETSTLDRLNALTRLGILLESKRQGILA
                     AYEALLRLRLRNQSDAIELGRPPDNWLDPKTLGRIEEAVLHECFNEIDLLQERIRRDF
                     LAG"
     gene            complement(2640..4262)
                     /locus_tag="P5179_09985"
     CDS             complement(2640..4262)
                     /locus_tag="P5179_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRS95588.1"
                     /translation="MMSYPLVLAFIVFFVAVTIVVTWFTQKYTKSTSDFYVAGKKVPW
                     VQTGIAMVGSYLSAASFLGCAGDISIFGVDRIWLSIGFFGGYMAVLMLIAGPLRNVGS
                     YTVADALHRRFPDRRIKLVVMITTVVISTFYLVPQMLGAGLLFEMLMGWDFVTVTAAL
                     GVLMSLYIIFGGMKATIYNQLIQAMFLFFAMTFITVFGFIMYYSGSLDALLGAMANIV
                     PPGICGKDAAAVAAVQSAVSSSAAVSVAREMLPTAPSALTIGIQTPDLVSQISTVVAL
                     VFGTAGLPHILIMFYTVRSASAARKSVTLCIVGLGIFYLCTILLGFILMPQVYPQLVT
                     WIAAGKQGLAKNMAVLQISGKVGGQWLMAVSAAGAVAAILSTSAGLMITVASTISHDF
                     YKSYINPNASERQEVNIAKVTTLVMSVISVVLAVAVKGENVAWLVTLAFGIAASAIFP
                     AMVTTLWWKRLTRQGAIAGMVTGLVVSVLFIVLLLGGVRTFLGLPTAGGPGVFGVTAS
                     FVVLLIVSYRTNDRGPEVEEFFALAHKDEEVE"
     gene            complement(4267..4527)
                     /locus_tag="P5179_09990"
     CDS             complement(4267..4527)
                     /locus_tag="P5179_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95589.1"
                     /translation="MLSPEQEKSSPPPGGATGPYRRKAILSFSVFGVFFVFYMGTAIL
                     QTPTFSKLAGIPWFGMPLGLLLSLLIFPVSWALIAVYFRLGR"
     gene            complement(4762..>5736)
                     /locus_tag="P5179_09995"
     CDS             complement(4762..>5736)
                     /locus_tag="P5179_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRS95590.1"
                     /translation="RAHALGENPQEVGCVGFNVVPENSDYNSFYFELAARLQWNPRAV
                     DLDEYVQKYCRLRYGPQYVAATEPVWRLLVETVYGEDSGTVKIIMDPLYWFRPDLRLL
                     AGWPEDDERTIGMWRRRSEFIPKLRRAAELLLAAGNLVGENNMARRDLVDIARQWIAE
                     RFNQALIGARDAFLAGNGGELARLEPICLRLLDDQARLLASWPAYRLSRQVAQVRDEY
                     PDPVKAVKLLHVWCNPVEGQESVPLRDYYRMDLDELVADYYKPRVAAYFALLRDKCAA
                     GRTTVTDEEFDAVYAPVERAFVAAPLCPLPDDEDPTDVVQDLLEDNVE"
BASE COUNT         1255 a         1822 c         1611 g         1048 t
ORIGIN      
        1 tcaacgaggt ccttgcgcgt gctcgcgtca caaacaaccc tgactcctcc cagttttcac
       61 ccagccaacg cctgacaacc tccgcggcga ttgacctcat tcaccgtgcg ggcgggtgtg
      121 cattcgtggc gcatcctctc cagactgagc cagacccggt tcgcttacgt tctctcctcg
      181 aggagctgcg cgggagcggt ctggacggcg ttgaggcctt ttacggtccg ctcctgccgg
      241 acaaaaggga ggcagttgcc caactcgcgc aaagcgccgg gctgctcgtc tgcggcggat
      301 cggatggcca cgggaacggc gaagcgggga tgggaattga catgccgctt ctccgttgga
      361 aggagtttcg gaacgctctc atcgaaggcg ccgcccagaa aacgcaccgc ggcccgtcac
      421 ctcagccggc tcggccagtg gaggaacgat tccactggcg cgccttcctg ttccatatcg
      481 ccatgccggc cttcctcgcc gtaacgctgt tcgtagtcgc ccttttcgta ctccttctgc
      541 cctattttga gcggacgctc atggatcgca agcgggagaa catccgggag cttacgcaga
      601 ccgcctggag cgttctggac gaagccgaac gggaggcctc cgctggcaac cttacccgcg
      661 agcaggcgca ggcgctggca atcaaacgcg tcgaagcgat gcggtacggg cccgaaggga
      721 aagattactt ctggcttcag gacctcacgc cacggatcat catgcaccct taccgccgcg
      781 acctgaacgg ccatgatgta tcgcaattca gggatcccaa cggcatcagg atattcgttg
      841 aattcgccga tctcgtgcgg aagcggggag agggttatgt aagctacgtg tggcaatgga
      901 aagacgaccc ggagcggatg gagcccaaag agtcctacat ccgacttttc gctccgtggg
      961 gatgggttat cggaacagga atctacgtgc acgacgtcca gcgcgagatt gcgggactgc
     1021 ggcaacggct ggtgaacgtc tctctgggtg tgacaggcgc cgtcctgctt ctgctcttgt
     1081 acattgtccg ccagagcgtc aaactcgaac gcgcccgccg cgacgcagag cggcgcctgc
     1141 gtgaatccac cgaccggtac cgggcgctca cttccgcagc caccgagggc gctctgttcg
     1201 tcgtggggag gcgttgcgcg tacgcgaacc ccgtgatgtc ggagataaca gggtacacgc
     1261 aaacccaact ggaactggtc gatgtcgacg atctcttccc cgcagttgag gcgaacatcg
     1321 aatggagaag gcgcctcgac aacccgggag gcgctgacac cgatatccct gtccccggag
     1381 tgctccagcg ccgcgacggg acccggttgg catgctccct cagtttgcgg agggtacctg
     1441 agagcccgcg cggttatatg attctggcgc gcaacgtcac ggcggcttct gaaccgccag
     1501 aggcccgcgc gctgctcaac cgactgctgc tcctgccaga aggcgtcgct tcggatctca
     1561 cggcggcaat ccagcatgca acgaagcccg cagacgtcgt tggcgcctgc ctcagggcgc
     1621 caaagctggt gcgcgcattg ctggaaaatg gcgcgtcgcc catgcttgtc actcgtgttc
     1681 tcgccgaact caccgacgaa gcgactcgac gcctgatctt ccttgcaaca gaagacattg
     1741 gacctgcccc gtgtccctac tcatttctcg cacttggcag ccaggggcgg cagtcacaaa
     1801 cgctgtacac cgaccaggac aacgctcttc tgtacagtct gacagattct gaagaccctg
     1861 aaacagccaa tcggtggttc ctggaactcg ggcagcgcgt gtgcacagac ctcgcccgcg
     1921 cgggattcag ggattgccgg ggcggctcca tggccagcaa cccccgttgg tgccagcctc
     1981 tgccggtctg gaagcgctat ttcgaggaat ggatccggaa cgccgaaccc cgtgaagtga
     2041 tggaattcag catcttcttc gacttccgat cggtcgacgg cgatgtcgaa cttccgcggg
     2101 aactccggcg acatgtaacc ggcgtgttga ggacggcacc tcactttttc gctcaggccg
     2161 cgcagaacgc gcttcagttc aaaccgcctc tccgcttgtt cggatccatt gtcggcggtc
     2221 gtgaccgcga acatagcggg cttctggacc tcaaatcggt gatgatgccg atagtgtctt
     2281 tcgcgcggct ctacgccctc cagcagggtg tcgaggaaac cagcactctg gatcgcctca
     2341 acgccctcac ccggctgggc attctgctcg aatcaaaacg gcagggcatc ctcgcggcgt
     2401 atgaggcgct cctccgcctc cgattgcgaa atcaatcgga cgccatcgaa ctgggaagac
     2461 caccggacaa ctggctcgat ccaaaaacac tggggcgcat cgaagaggcc gtgctgcacg
     2521 agtgtttcaa cgaaatcgac ctgctccagg agaggattcg aagggatttt ctggcggggt
     2581 aaatgaaaaa agcccgccac ggcaaggacc atggcgggct ttcgcgagga ccggaacgat
     2641 tactcgactt cctcatcctt gtgggcaagg gcgaagaact cctccacctc tggccccctg
     2701 tcattcgtcc tgtagctgac aatcagcagt acgacaaacg aagcagtcac gccgaacaca
     2761 cccgggcctc ccgcagtcgg taacccaagg aacgtccgaa ctccgcccag caacaacacg
     2821 ataaacaaaa ccgagactac gagcccggta accattccgg caatcgcccc ctgccgagtc
     2881 aggcgcttcc accacagtgt cgtaaccatc gcagggaaaa tcgcgctggc ggctatacca
     2941 aatgccagag taaccagcca ggcgacgttc tcgcccttca ctgcgaccgc aagcacgact
     3001 gagatcaccg acatcacgag agttgtgacc ttcgcgatgt tgacttcctg tctctcactc
     3061 gcgttcgggt tgatatagct cttgtagaag tcatgactta tcgtcgaagc aaccgtgatc
     3121 atcaacccgg cggatgtact gagtatcgcg gcgaccgcac ccgccgccga aacggccatc
     3181 aaccattgcc cgccgacttt cccgcttatc tgcaacacgg ccatattctt cgccagtcct
     3241 tgtttgccgg cggcgatcca cgtgaccagt tgaggatata cctgaggcat taggatgaac
     3301 cccagcagga tcgtgcagag gtagaaaatc cccaggccga cgatacacag cgtaacgctc
     3361 tttcgcgccg cgctcgcgga acggacggtg taaaacatga taaggatgtg cggcaggccg
     3421 gccgttccaa aaaccagcgc cactaccgtg ctgatctgag aaaccaggtc gggtgtctgg
     3481 atcccgatcg tcagtgcact cggagcggtg ggcagcatct cccgcgcgac actgaccgct
     3541 gcggaagagg atactgctga ctgaaccgcc gcaaccgcgg cggcgtcttt gccgcagatc
     3601 cccggcggca caatgttcgc catcgcaccc aacagcgcat ccagcgaccc gctgtagtac
     3661 atgatgaagc cgaacaccgt gatgaaggtc atggcgaaaa acaggaacat cgcctggata
     3721 agttgattgt aaatggtcgc tttcatcccg ccgaagatga tgtagaggct catcaacacc
     3781 ccgagggcag cggtgacggt gacgaaatcc caccccatca gcatttcaaa cagcaaaccc
     3841 gcacccagca tctggggaac gagatagaag gtcgagatga ccaccgtggt aatcatcacc
     3901 acaagcttga tgcgccggtc ggggaacctc cggtgaagcg catcggccac ggtgtagctg
     3961 ccgacattcc gaagcggacc cgcaatcagc atcagcaccg ccatatagcc gccgaaaaag
     4021 ccgatcgaaa gccatatccg atcaacaccg aaaatcgaga tgtctcccgc gcatccgaga
     4081 aagctcgcgg cggagaggta gctccccacc atggctatcc cggtctgaac ccagggaacc
     4141 ttcttgcccg ccacgtagaa atccgaagta gatttcgtgt atttctgggt aaaccatgtt
     4201 accacgatcg tcacggcaac gaagaagaca atgaacgcca aaaccagcgg gtagctcatc
     4261 atgttgtcat ctccccagcc tgaagtagac tgcgataagc gcccaggaaa ccgggaaaat
     4321 gagaagcgac aacaacaaac cgagcggcat gccaaaccat ggtatccccg cgagcttgct
     4381 gaacgttggc gtctggagaa tcgccgtccc catgtagaag acgaagaata cgccaaaaac
     4441 ggaaaagctg agtatcgctt tccgccgata cgggccggtc gcgcctcccg ggggcggcga
     4501 cgatttctcc tgttcgggac tcaacatgaa agcactcctt ttcccagtgg ggagagttga
     4561 aggctacgcg gaaaccggaa gaaaggagta tatgacgcgt gagttggcgg gtcaagcacg
     4621 ttgaacacgc ttgcgcgtat gaaacttgtt tcgctgggcg gctctttcct gccgggattg
     4681 cccggcatct tctcccgcag aaaaggctct tccagacggt ccgccgcaaa aaaaatagta
     4741 tcggagccga ttacctttcg gtcactccac attgtcctcc aacaaatcct gaaccacatc
     4801 ggtcggatct tcgtcgtcgg gtaacgggca gagcggcgcc gcgacgaagg cacgttccac
     4861 aggagcatac acggcgtcaa actcctcatc cgtcactgtt gtccggcccg ccgcgcactt
     4921 gtcccgcaac aacgcaaaat aggccgccac ccgtggcttg tagtaatcgg cgaccagttc
     4981 gtcaaggtcc atccggtaat agtcccgcag gggcaccgat tcctgccctt ctaccggatt
     5041 acaccacacg tggaggagtt tgaccgcttt gaccgggtcc ggatactcat cgcgcacctg
     5101 cgcaacctgt cggctgagac gatacgcggg ccagctcgcg agaagacgcg cctggtcatc
     5161 gagcaacctg aggcagatcg gctccagacg cgccagttcc ccgccattcc ctgccagaaa
     5221 cgcatcacgc gcgccaatca gcgcctgatt gaaacgctcc gcgatccact ggcgggcaat
     5281 atccacaagg tcccgccgcg ccatattgtt ctcacctacc agattgccgg cggcaaggag
     5341 aagctcagcc gcgcgacgaa gcttcgggat gaactccgaa cggcgtctcc acatcccgat
     5401 agtccgctca tcgtcttccg gccacccggc cagaagcctc aggtcggggc ggaaccagta
     5461 gagtgggtcc atgatgatct tcaccgtacc gctgtcctcg ccgtacaccg tctccacaag
     5521 aagccgccac accggttcgg tcgccgcaac gtactgcggc ccgtagcgca acctgcagta
     5581 cttttgcacg tactcatcca aatccaccgc gcgcgggttc cactgcaggc gagccgccag
     5641 ttcgaagtag aacgaattgt agtctgagtt ctccggcacg acgttgaacc ccacgcaccc
     5701 gacctcctgc gggttctctc cgagtgcgtg cgcgcg
//
LOCUS       DAOTNG010000205         5690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_11980_length_5690_cov_5.059095, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000205 DAOTNG010000000
VERSION     DAOTNG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11980_length_5690_cov_5.059095"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="P5179_10000"
     CDS             complement(<1..454)
                     /locus_tag="P5179_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS95591.1"
                     /translation="MLESRERVIPIQIEEEIRESYMEYALSTLVARALPDVRDGLKPS
                     QRRILVAMNDLHLGPTAKYYKCAKVAGDTSGNYHPHGEAIVYPTLYRMAQPFNMRYRL
                     VDGQGNFGSIDGDPPAAMRYTECRMTSVATELLADLDRDTVDFQSNYDE"
     gene            complement(579..2615)
                     /gene="gyrB"
                     /locus_tag="P5179_10005"
     CDS             complement(579..2615)
                     /gene="gyrB"
                     /locus_tag="P5179_10005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRS95592.1"
                     /translation="MSEELHLNNHGIPESTPENANGHLSGKANGNGIGNGSTAVDTDT
                     AYDANSIQVLEGLEAVRKRPGMYIGSTGTQGLMNLIREIVDNSIDEALAGRCDRIEVV
                     LLPDGSARVSDNGAGIPTDIHPTEGVSAVEVVMTKLHAGGKFGHSAYKVSGGLHGVGA
                     SVVNALSEWCIVEVHQKGRAFRQRFERGTTVTPLEDVGEAQDTGTITTFKPDATIMTE
                     TEFAYEPLMHTLREQAFLNRGVTIVLRDERGEEVREDVFRFDGGIAEFVQFLDEGKTP
                     LHTPPIYIERMRDDVIVELAMQYNASYSEAVFTYVNNINTVEGGTHLAGLRAALTTSI
                     NAYAQANNLLKDMKEGLSGEDVREGLTAILSLKIPNPQFESQTKIRLGNPEIKGLVQS
                     VVTEGLNEYFDENPQVIKRIIMKSVEAAHARLAARKARELTRRKSILDGGGLPTKLSD
                     CSITDPAACEIYLVEGDSAGGSAKQARDRRYQAVLPMWGKMLNVEKARIDKVLGNDKL
                     QPVIQALGASIGEEFDLSKLRYHKVIIMADADVDGSHIRTLILTFFFRFMRPLIEAGH
                     VYIAQPPLFLVKRGKEEHWVFSDAERDAALARFENQKGVTVQRYKGLGEMSAEQLWST
                     TMNPETRTLLRVSMEDAVEADRIVTVLLGADVEPRKRYIEANAQYVKELDIHAA"
     gene            complement(2608..2988)
                     /locus_tag="P5179_10010"
     CDS             complement(2608..2988)
                     /locus_tag="P5179_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRS95593.1"
                     /translation="MSDSAGNDQRAGTTLPLQTVLQRMAGQWQVRQASALEILKEHWD
                     EVTGEVWARTITPEMVDKKGRLVVTVRSAPGVAMEIQRKGRRWRQILEGTIRVSGYEF
                     TDVVLAPPYSLRKRTTRERTRGNE"
     gene            complement(2981..4099)
                     /gene="recF"
                     /locus_tag="P5179_10015"
     CDS             complement(2981..4099)
                     /gene="recF"
                     /locus_tag="P5179_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRS95594.1"
                     /translation="MHGIQAVHLTSVQLRDFRSYRSASAVFAPALNIVSGENGQGKTN
                     LLEAIRLISVGRSFRGATDGEMVSWGQPSGTITGTIGDGPVRQIVLTITPGERKRGTI
                     DGAALNGLAELVGVLRTVAVSSDEIEQQFRSQTGRRRMIDVMISQVDRRYLTELRRCK
                     TIVAQLNAVYRDPANDDALCDVLEHQLAEVSLRINRRREAFFRDFCGAVEGHFSRLFG
                     IGNLVLESRTTLPGDGDMSDLDRAVDVLRESRPSARRAGFAVRGAHRDRYRVLLDGKD
                     IESHASKGQVKIAYLAWKLAEGDFIERETTRSPVWLIDDPFSELDAKRAAALLASTEQ
                     RGQVILTNARTDDVAVLKNGPAHHLRVHDGIVEGVHGV"
     gene            complement(4149..5276)
                     /gene="dnaN"
                     /locus_tag="P5179_10020"
     CDS             complement(4149..5276)
                     /gene="dnaN"
                     /locus_tag="P5179_10020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRS95595.1"
                     /translation="MRVQMDQSELLGMLQTVIDAVPSKSTLPILSMMLMVIEEGTCSL
                     SGTDLEVFLTTTRAVQDAEDGRITFPGRLMFDFVRELPPGPITISEADGRVTLKSANG
                     ECSLLGMPANEFPTVPKGIEGGVTISVDGDLLRRMIEKTAFAVHPDQTRPVLSSICWR
                     VNPNGMVMVATDGHRLAKISEVVEGIHGDPLQVIVAPRVLQQVERLIRDDNKLESITI
                     GERQIHFAYSGANLVTRLVEGKYVDVESVIPKDNNRRLIVSKEELQPAVRRMQILSSQ
                     QNHQVRLALHKDELELSTVNRDTGAEAHERISAQYDDEEMEIGYNANYLKEVLSKIDS
                     DQVEFHFREPVSAAIIKPAKQPEGEDYFCLLMPLRLLNTDS"
BASE COUNT         1071 a         1746 c         1555 g         1318 t
ORIGIN      
        1 gttcgtcgta gttggactgg aagtctacgg tgtcccgatc cagatccgcg agcaattccg
       61 tggcgacgga cgtcatgcgg cactcggtgt atcgcatggc cgccgggggg tcgccatcta
      121 tggaaccgaa gttgccctgt ccgtcaacca gccggtagcg catgttgaag ggctgtgcca
      181 tccgataaag cgtcggatac acgatcgctt caccgtgggg atgatagttc cccgaagtgt
      241 cgcccgccac cttcgcgcat ttgtaatact tggccgttgg cccgaggtgg aggtcgttca
      301 tcgcgacgag aattcgtcgc tgagacggct tcaaaccgtc gcgcacgtcc ggcaacgctc
      361 gcgcgaccag cgtgctgagc gcatactcca tatacgattc gcggatttct tcttcaatct
      421 gaatcggaat gactcgttct cgagattcga gcatggaaac tccctcaaat tgtgctcgtt
      481 tttactcaac caccgagtac ggacaaagcg tggaaagctt tgtccgcaca tgcacgacgg
      541 cgtccgcccg ggtgatgaac aactgatcgg cggcgccgtc aggcggcgtg tatgtcaagc
      601 tctttcacgt actgtgcgtt cgcctcgatg taccgttttc tgggttccac atcggcaccc
      661 agcaggaccg tcacgatgcg atctgcttcc accgcatctt ccatggagac tcgcagcaga
      721 gttcgcgtct ccgggttcat ggtcgtgctc cagagctgtt ccgcgctcat ctcacccaga
      781 cctttgtacc gttgcactgt gacgcctttc tggttctcga aacgcgcgag cgcagcgtct
      841 cgctcggcgt cgctgaacac ccagtgctcc tcctttcctc gtttgacgag gaaaagaggt
      901 ggttgagcga tatatacgtg tcccgcttcg ataagcgggc gcatgaaccg gaagaagaac
      961 gtcaggatca gagtgcggat gtgcgagcca tcgacgtccg cgtcggccat gataattact
     1021 ttgtggtagc gcagctttga caggtcgaac tcttcaccga tgcttgcgcc cagagcctgg
     1081 attaccggtt gcagcttgtc gttgccaagc accttgtcaa ttcgcgcttt ctctacgttg
     1141 agcattttgc cccacatggg aagcacggct tggtaccgac ggtctcgcgc ctgttttgcg
     1201 gatccccctg cggaatcacc ctcaacgagg taaatctcgc aggccgcagg gtcggtaatg
     1261 gagcagtcgg acagcttggt cggaaggccg ccgccgtcca gaatgctttt ccggcgcgtc
     1321 aactcgcgcg ccttgcgggc ggccagcctg gcgtgcgccg cctccacgga cttcatgatg
     1381 atccgcttga tcacctgagg gttctcgtcg aaatactcgt tcaacccttc ggtaaccact
     1441 gactgcacca accccttgat ctcgggatta cccaggcgga tttttgtctg gctttcaaac
     1501 tgaggattcg gaatcttcag gctcaggatc gcggtcagac cctctcgaac atcctctccc
     1561 gacaggcctt ccttcatgtc tttcagaagg ttgtttgcct gtgcgtaggc atttatcgac
     1621 gtggtgagcg ccgctctcaa tcctgcgaga tgggttccgc cctccaccgt gttgatgttg
     1681 ttcacatatg tgaaaacggc ctcgctgtac gatgcgttgt attgcatcgc gagttccaca
     1741 atcacgtcgt cgcgcatgcg ctcgatgtag atcgggggtg tatggagggg cgttttcccc
     1801 tcgtcgagaa attgaacgaa ttcggcgatg ccaccgtcga accggaacac atcctccctg
     1861 acttcctcac cgcgttcgtc gcgcaggacg atggtaacgc ctctgttcaa aaacgcctgt
     1921 tcccgaagag tgtgcatgag cggttcgtac gcgaattccg tttccgtcat gattgtggcg
     1981 tcaggcttga acgtggtgat ggtcccggta tcctgcgcct ctccgacgtc ttccaacggc
     2041 gttaccgtag tgccacgttc gaaccgctgc cgaaatgcgc gtcccttctg gtgcacttcc
     2101 acgatgcacc actctgaaag cgcattgacc acgctcgcac cgacaccatg gagtccgccg
     2161 gagaccttgt aagccgaatg gccgaatttg cctcccgcgt ggagcttcgt cattaccacc
     2221 tcgacggcgg ataccccttc cgtcggatga atatcggttg gaatgccggc tccgttgtcc
     2281 gaaacacgcg cggatccgtc gggcagaagg acgacttcga tgcggtcgca ccttcccgcc
     2341 agtgcttcat cgattgagtt gtccacaatc tcgcggatga ggttcatcaa cccctgtgtg
     2401 ccggtggagc cgatgtacat ccccggacgc ttgcggactg cttccagacc ctcgagcact
     2461 tgaatgctgt ttgcgtcgta ggcagtatca gtgtcaaccg cggtcgagcc gttgccgatc
     2521 ccgttcccgt tggctttgcc ggacagatgc ccgttggcgt tttcgggggt gctctcgggt
     2581 attccatggt tgttcaggtg caactcttca ctcattccct ctggtccttt cccgcgttgt
     2641 tcgctttcgc agcgaatatg gcggagccaa taccacgtcg gtgaactcgt atccgcttac
     2701 ccggatggtg ccttccagga tttgccgcca ccggcggcct ttcctctgaa tttccatcgc
     2761 tactccgggg gcggatctga ccgtcaccac cagccgtccc tttttgtcta ccatttccgg
     2821 ggtgatcgtg cgtgcccaca cttccccggt aacttcgtcc cagtgttcct tgagtatctc
     2881 cagcgccgag gcctggcgca cctgccactg ccccgccatg cgctgcagaa cggtctgcaa
     2941 cggcagagtt gtgccggccc tctggtcgtt gccagctgaa tcagacacca tggacgcctt
     3001 caacgatgcc atcgtgaacc cgcaaatggt gggcgggccc gtttttcagc actgccacgt
     3061 catcggtccg ggcatttgtc agaatgacct gcccgcgctg ttctgtcgag gcaagcaacg
     3121 ccgccgcccg cttcgcgtcg agctcgctga aagggtcgtc aatcaaccac acgggcgagc
     3181 gcgtcgtctc gcgttcgatg aagtctccct cagcgagttt ccacgcgagg tacgcgatct
     3241 tgacctgccc cttcgatgcg tgcgattcga tatctttccc gtcgagcaga acccggtaac
     3301 ggtcccgatg cgcgccgcgc actgcaaatc ccgcacgccg ggctgaggga cgggattcgc
     3361 gcaacacatc cactgcgcgg tccagatcac tcatgtcccc atcacccggg agcgtggtgc
     3421 gcgattccag cacgagattg ccgatcccga acaaacggga aaaatgccct tcaacggcgc
     3481 cgcagaaatc gcgaaaaaac gcttctcttc gcctgttgat gcgcagcgat acttctgcca
     3541 gctggtgctc caatacgtca cacaacgcat catcattggc cgggtctcga tacactgcgt
     3601 tcagttgtgc aacgatcgtt ttgcaccggc gcaactccgt caggtaccgg cgatccacct
     3661 gtgaaatcat gacgtcgatc atccggcgcc ggccggtctg cgaccgaaac tgctgttcaa
     3721 tctcgtcaga ggatacggcc acggtgcgga gcacgcccac caactccgcc agtccgttca
     3781 acgcagcacc gtctatcgta cctcgcttcc gttctcccgg ggtgatggta agaactatct
     3841 gacgcacggg cccatcgccg atggtgccgg ttatcgtacc ggatggctgc ccccacgata
     3901 ccatctcccc gtcagtggcg ccgcgaaagg aacgcccgac agagatgagg cgtatcgcct
     3961 cgagaaggtt tgtttttccc tgaccatttt cccctgaaac gatgttcagc gccggagcga
     4021 acaccgccga cgctgaacgg taacttctga aatcgcgcaa ctggacggac gtgagatgca
     4081 ccgcctgaat cccatgcacg ctttttgaat ccgtttccgc gcccggcggg atagcgtgcg
     4141 gtcgcgtgct acgaatcagt gttgagcagt ctcaacggca tgagcaagca gaagtaatcc
     4201 tcgccttccg gttgcttggc gggttttatg atcgcagcgg aaaccggttc gcggaaatgg
     4261 aattcaacct gatcggaatc gatcttggac agcacctctt tcaggtaatt cgcattgtac
     4321 ccgatttcca tttcctcgtc gtcgtattgt gcagaaattc tttcgtgggc ttccgcaccc
     4381 gtatcacggt ttaccgtgga gagttcgagc tcatctttgt gaagtgcgag acgcacctga
     4441 tgattttgct gactggacaa tatctgcatg cggcgaacgg ccggttggag ttcttccttg
     4501 gacacgatca ggcgccggtt gttgtccttc ggaatcaccg actccacatc gacgtactta
     4561 ccttccacca gccttgtcac aaggttcgcg ccggagtagg cgaaatgtat ctgccgctcc
     4621 cctatcgtaa tgctttccag tttgttgtcg tcacgaatca gccgttctac ctgctggagc
     4681 accctcggcg cgacgatgac ctgaagtgga tctccgtgga tgccttcaac cacctcgctt
     4741 atcttcgcga gcctgtgacc gtcggtcgca accatcacca tcccgttcgg attgacgcgc
     4801 cagcagatgc tgctcaacac gggtcttgtt tgatcgggat gcacggcgaa tgcggtcttc
     4861 tcgatcatac gcctcagcag gtcgccgtcg acggagatgg ttacgccccc ttcgataccc
     4921 ttcggaaccg tggggaactc gttggccggc atgcccagca ggctgcattc gccattcgcc
     4981 gatttgagcg taacccggcc gtctgcctcg ctgatcgtaa tggggccggg cgggagttct
     5041 cgcacgaaat caaacatcaa ccggccggga aaggtaatcc ggccatcttc tgcgtcctgg
     5101 actgctcggg ttgtggtgag gaatacctca aggtcggttc ccgaaagcga acacgttcct
     5161 tcctcgatga ccatcagcat catggagagg attggcaacg tgcttttgct cggaacggca
     5221 tcaatcacgg tctgaagcat accaagcagt tcactttgat ccatctggac gcgcatagaa
     5281 agcccttcct ttcccgaaaa aaccaattaa caaaagatac gcaaacccat ggagatttcc
     5341 aacgtgccgc agaacgcagt ttgatgcttc ccgtggggat ttccgacgca tcaccggtgt
     5401 gtcagtcggc cgtttgaatt taccctcgca gaaggcggcg cgctcagcgg gaaatttcgc
     5461 gttgcgcgtt tctccgggtt gggaacagcc agagcgctgc acacggtgct tcagtaacgc
     5521 gtcatacctg tgccgagcct acagggacag gcctcggagt acgatttccc gtttcttcgc
     5581 gcttcgtcgt gtgattgtca ggcgaatagt gtgggatggt gcccattctt cggccagctc
     5641 cggccattcc accagggcta tgccgtcgcc gaaaaatacc tcctccagcc
//
LOCUS       DAOTNG010000206         5681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12005_length_5681_cov_4.510487, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000206 DAOTNG010000000
VERSION     DAOTNG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12005_length_5681_cov_4.510487"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="P5179_10025"
     CDS             complement(<1..1195)
                     /locus_tag="P5179_10025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS95596.1"
                     /translation="MANRVPPSKHQKTRSKPLPPPTVKNKPAPYNIAPQLPAWFGSPF
                     IPAGVIVAYVIVWGVFSVGFWRVGGYGVETDAFGSFLPEVRKMLRGEFTPLDGFKGPG
                     YHAAVALVSFVARDLFLSAKIVALASSAVVLWLLFRLVRRSLGTGTAWVAMLFVATNV
                     QFILFTIQVGTDMFFLAVAIAAATLILERGGWQRSLAAGLMAGFAYLTRYNGVFLTLG
                     GIVIMALADRDTLGGRQRLLRTVAFLGGVLVMFAPWSYYTWSQGLGFFFNANHLNIAY
                     EMFGRDTVSWDQFWEYFNPAFASFGEVVRGNVGRFVAMLARNSIEHFWWDMRRVLVAG
                     TAPWVVPLSIVWLAMIAYGLVRVVIMKRERAWPAIVLGVGAYGVLVPVFYGERFSLPM
                     VPIYAV"
     gene            1618..2559
                     /locus_tag="P5179_10030"
     CDS             1618..2559
                     /locus_tag="P5179_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRS95597.1"
                     /translation="MSYQLPAIDRLGFCNVFLSAIGGDGANMSAKLLFKIAVTELDLD
                     GAYDAKYGSEKKGTATDVSLRLCYKGTAVRTSGPTTTPHILAIFRPGLIETLQLNRGL
                     QANATVIVNTTQTPDEIRDRLKMHSGRIVCVDAETIAEDTGSRLNMPMMALVCRALHF
                     PQEQVEAVIAKTWPYAKDANLGAFKAAYDGVNEKEFAPRADYPLLPPALVARGQIGYL
                     NMLNGGAIDAMTHSTVNRNNQVAGYGFVPTFDPSACTGCAVCMTVCSDPGGIVWKDGK
                     VIGIDYAFCKGCMRCVEVCPQTKKGKALNRPEIAQVA"
     gene            2623..4824
                     /locus_tag="P5179_10035"
     CDS             2623..4824
                     /locus_tag="P5179_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRS95598.1"
                     /translation="MVPQKSLIANGNDAVAQAIVDIGYDGEGYYPITPSSDAGERVSE
                     ALAAGKTDINFVIGTSELAVAGIITGMAVAGGRAVDVTSANGLLLKMEELPCISGLGL
                     PLVMNIATRDVSGPLNIKNGHSDLAAVLGVGWLIFLAPTVQATYDLNLIALKAAEAVS
                     LPAFVAYDGFHTSHGVRRINVFANQEDARAFVGKKPEMLNLLDVHHPHTFGPYMNDDL
                     INTKVQLDAKMKKAYELLPGIFKEFEELTGRHYSFVESYGSDNPDAVLVALNSAGEAA
                     KVAVDDMIADGKKVRVVIPTVLRPYPNDEFVDAVKGAKTIVVAERVSQYGAGNYLANE
                     LGASLQRIGSKSTILSRVYGIGGLNYLPEDGVHMLTTALAYPNVPEEEKKIHAYHGAW
                     PGDPSYKPLPTIQPLTAEETTLNAGEERVNLRKLAEMPTRIEKHSACPGCGIFTNLNL
                     FLRGIDGHVVLLFNTGCGMVVTTGYPLTSFKVPYFHNLFHNGSSTATGVVEMYNRFRK
                     QGKINEEITFIVVSGDGGDDIGMDQVIGSALRNDPFIMLEYDNKGYMNTGGQLCYTGF
                     KGQRNSNAPIGPALRGKKTHHKDVIEILRGTECSYLFQAAESNARDMIAKARKAQATV
                     RAGGFAFGKVFSVCPLNWGADEAKGNEIVEKVVDACMHPLYEVEHGVTRLSYDPEERG
                     KKIPVGDAFRVMGRAFGHLLKPDNADLLQEVQDEVDRRWERLKAMSSSEIL"
     gene            complement(4900..5556)
                     /gene="nth"
                     /locus_tag="P5179_10040"
     CDS             complement(4900..5556)
                     /gene="nth"
                     /locus_tag="P5179_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRS95599.1"
                     /translation="MATGKRSARAEKERVAEIHRRINAEYPRSTTALEYRTPVQLLVS
                     TILSAQCTDVRVNEVTKSLFQNYRTARDFAEAPIEELEEAIRPTGFFRNKAKNIKNCC
                     KTIVERFGGEVPRNMDDLVQLDGVGRKTANCVLGNAFGMNEGVVVDTHVMRLSKRLGL
                     SASEDPVKIEQDLMRLFPRESWTLLAHQLIDHGRAVCNARKPKCGECLLADLCPSAKP
                     "
BASE COUNT         1311 a         1643 c         1620 g         1107 t
ORIGIN      
        1 gaacagcata gattggcacc atcgggagac tgaagcgttc cccgtaaaac acaggaacga
       61 gaacgccgta cgccccgacc cccaacacta tcgcaggcca ggcgcgctcg cgcttcatta
      121 tcaccacgcg gacgaggcca taggcgatca tcgccagcca cactattgaa agcggcacca
      181 cccagggcgc tgttcccgca acaaggacgc gacgcatatc ccaccagaaa tgctcgatgc
      241 tgttgcgcgc cagcatcgcg acaaatctcc cgacgttacc ccgcaccact tccccaaagg
      301 aggcgaatgc ggggttaaaa tactcccaga actggtccca cgacacggta tctcgtccga
      361 acatctcata cgcgatgttc aggtggtttg cgttgaaaaa gaacccgagg ccctggctcc
      421 atgtgtaata agaccaaggt gcaaacatca ccagaacccc gccgagaaac gctacggtcc
      481 tcagcagacg ctgcctgccg cccagtgtgt cacggtcggc caacgccata attacgattc
      541 cgcccagcgt cagaaacact ccattgtacc gcgtgagata ggcgaatccg gccatcaaac
      601 ccgccgcaag agatcgttgc caacctcctc gctccagaat cagggtcgcc gccgcaatcg
      661 cgaccgcgag gaagaacatg tcagtaccaa cctggatcgt gaagagaatg aactggacat
      721 tcgtggcaac gaacaacatg gctacccacg cggtgccagt tcccagcgag cgccgcacaa
      781 ggcggaacaa cagccagaga acgaccgcgg aagatgccag agccacgatt ttcgcggaga
      841 ggaagaggtc gcgcgcaaca aagctcacca aagctaccgc agcatgatat cccggacctt
      901 tgaacccatc caacggagtg aattcgcccc gcaacatctt cctgacttcg gggagaaagc
      961 tcccgaacgc gtccgtttcg actccgtacc ctcctaccct ccagaatcca acgctgaaaa
     1021 cgccccacac gatgacatat gctacaataa ccccggccgg aatgaatgga gaaccaaacc
     1081 acgcaggtag ttgtggcgca atgttatatg gcgcaggttt gtttttcacc gtggggggag
     1141 gtagaggttt ggatctcgtt ttttggtgtt tggaaggcgg tacgcgattc gccacggtta
     1201 cctcctggcg caacaaagtc cggcatgcgt gcttgttcga aaggctcgaa aatactgtac
     1261 gaccgcggac gctgttcgcc aagcacagga gccacaggct gcttcacgag cggggagacg
     1321 aaatcgcggg aaggggacaa acggggcttg cttagcgcac acgtcgaacc gcgtcaaacg
     1381 gcagcaggac tgtaacgaaa caggacaccg catgccaagg ccagccgaag caatgctgag
     1441 atatggcgcc tgctacgccg ttttcaaccg tcttcagggg tttctgcccg gcataggatt
     1501 tgcgatattg tacaatctac ccatcgaagc gctttcacgt cccgacgtga tccgtcgggc
     1561 acacatcgca tcaaccgcat cacacgttga tgcttttctc cgcaggagga atcctccatg
     1621 tcataccagt tgccggcgat agatcggctc ggtttctgta acgtgtttct ttccgccatc
     1681 ggtggtgacg gcgcgaacat gtccgccaag ttgctgttca aaatcgccgt gacggaactg
     1741 gacctggacg gcgcgtacga cgcgaaatat gggagcgaaa agaaaggcac tgcaaccgac
     1801 gtttcgctcc gcctttgcta caaaggcaca gccgttcgga cgtccggacc caccaccacg
     1861 cctcacatcc ttgcgatttt ccgtccgggg ttgattgaaa cactccaact gaatcgcggg
     1921 ctccaggcga atgcaacggt catcgtgaac accacgcaga caccggacga aatccgggat
     1981 cgcctcaaga tgcattcggg cagaatcgtc tgcgtcgacg ctgagacgat tgcagaagat
     2041 acgggctcgc ggctcaacat gccgatgatg gcgctcgttt gccgcgcact ccacttcccc
     2101 caggagcagg tagaggccgt gatcgccaaa acatggccct acgcgaagga tgccaatctc
     2161 ggggcattca aagccgcgta cgacggcgtc aacgaaaaag agttcgctcc tcgggccgac
     2221 tatccactcc ttccaccagc cctggttgcg cgtggccaga tcggatacct caacatgctc
     2281 aatggtggcg ccattgacgc gatgacccat tccacggtca accggaacaa ccaggttgcc
     2341 gggtacgggt ttgtcccgac attcgaccct tcggcgtgca ccggctgcgc ggtgtgtatg
     2401 actgtctgct ctgacccggg cgggatcgtc tggaaggacg gcaaggtgat cggcatcgat
     2461 tatgcattct gcaaggggtg catgaggtgt gttgaggtat gtccgcagac caagaagggc
     2521 aaagccctga atcgtccaga gattgcgcaa gtagcctgat cgttctttcc ggctcggaga
     2581 ggcttgatcc cttctccgac ctgcaacagg gagtggcaca cgatggtacc ccagaaatcg
     2641 cttatcgcga atggcaacga cgctgttgcc caagcgattg tagacattgg ctatgacggt
     2701 gagggctact acccgattac accttcatct gacgcaggtg aacgcgtcag cgaggcactt
     2761 gccgcgggca aaacagacat caatttcgtc ataggtacat cagaactggc ggtcgccggc
     2821 atcatcacgg gaatggcggt tgcggggggc agagccgttg acgttacctc ggcgaacggg
     2881 cttctcctga aaatggagga acttccctgc atcagcggac tcggcctgcc gctggtcatg
     2941 aatatcgcaa ccagggacgt cagtggccct ctgaacatca aaaatggaca cagcgacctt
     3001 gcagcagtgc tgggcgttgg ctggttgatt ttcctcgcgc cgaccgttca ggctacgtat
     3061 gacctgaacc tgattgcgct gaaggctgcc gaggcagtct ctctgccggc gtttgtcgcg
     3121 tacgacggct tccatacaag ccatggcgta cgccgcatca atgtgttcgc caaccaggaa
     3181 gatgcgcgag cgttcgtcgg gaaaaaaccc gaaatgctga acctgctgga tgtacaccat
     3241 ccgcacacgt tcgggcccta catgaacgac gacctcatca acacgaaggt ccagcttgac
     3301 gcgaagatga agaaagcgta cgagcttctg cccggcatct tcaaggaatt cgaggagctc
     3361 acagggcggc actacagctt cgtggaaagc tacggctctg acaacccgga cgcggtgctt
     3421 gtggccctga acagtgccgg agaagcagcc aaagtggctg ttgacgacat gattgcggac
     3481 ggcaaaaagg tcagggtggt catcccgacc gttctgcggc cttatcccaa cgacgaattc
     3541 gtggatgcgg tcaaaggtgc gaagacgatt gttgtggccg aacgcgtttc gcagtatggg
     3601 gcaggcaatt atctggcgaa tgaactgggc gcgtcattgc agagaattgg ctcgaagagc
     3661 acgattttga gccgggtgta cggcatcgga ggtctgaatt accttcccga agacggcgta
     3721 cacatgctca ccacggcgct cgcctacccg aacgttccgg aagaagaaaa gaaaattcac
     3781 gcataccatg gtgcgtggcc gggcgatccc agttacaaac cactcccgac gattcagcct
     3841 ctcaccgcag aggagaccac gctgaacgcg ggtgaggaac gcgtgaacct ccgcaaactt
     3901 gccgagatgc cgacgcggat cgagaaacac tcggcgtgcc cgggatgtgg gattttcacc
     3961 aatctgaacc tgttccttcg cggaatagac gggcatgtag tccttctgtt caataccggt
     4021 tgcgggatgg tggttacgac ggggtacccg cttaccagct tcaaggttcc ctacttccac
     4081 aacctcttcc acaacggttc ctccactgca accggcgtgg tggagatgta caatcgtttc
     4141 cgcaagcagg ggaagatcaa cgaggagatc accttcatcg tcgtgtcagg cgacggtggc
     4201 gatgacatcg gaatggacca ggtgatcggt tcagcgcttc gtaacgaccc gttcatcatg
     4261 ctggagtacg acaacaaagg ctacatgaac accggcgggc agctctgcta caccgggttt
     4321 aagggccaac ggaactccaa cgccccgatc gggcccgccc ttcgcggtaa gaagacgcat
     4381 cacaaggacg tgatcgaaat actccgcgga acggaatgct cctacctttt ccaggcggct
     4441 gagtcgaacg cccgtgacat gattgcgaag gcccgcaaag cgcaggcaac tgtgcgtgcc
     4501 ggtggtttcg catttggcaa ggtgttcagc gtctgcccgt tgaactgggg agcggatgag
     4561 gcgaagggta atgagattgt cgaaaaggtg gtggatgcgt gcatgcaccc gctgtacgag
     4621 gtcgagcacg gtgtcacacg actgagttac gacccggaag agcgaggcaa gaagatcccc
     4681 gtcggcgacg cgttccgcgt gatgggacgc gcatttgggc acctgctcaa gcccgataac
     4741 gcggatctcc tgcaggaggt tcaggacgag gtggatcgtc gttgggagcg tctcaaggcg
     4801 atgagttcat cagaaatcct gtaaaaacag caacgaaaag gcaagacgct tcggggaacc
     4861 attatccccg aggcgtcttg ttttgtgaag gctgggaaat cagggcttgg ctgaggggca
     4921 tagatcggcg agcaggcact cgccgcattt cggtttgcgg gcattgcaca ccgcgcggcc
     4981 gtggtcgatc agctggtggg cgagcagggt ccagctttcg cgaggaaaaa ggcgcatcaa
     5041 atcctgctcg atcttcacgg gatcttcaga agcgctcaag cccaggcgtt tcgacagccg
     5101 catgacatgg gtatccacca ccactccttc gttcatcccg aacgcattcc ccagcacgca
     5161 gttggctgtt ttgcggccaa ctccgtccag ttgcaccaga tcgtccatgt tccggggaac
     5221 ctcaccaccg aagcgttcca cgatggtttt gcagcaattc ttgatgtttt ttgccttgtt
     5281 gcggaaaaag ccggtcggtc ggatcgcttc ctccagttcc tcgattggcg cttcagcgaa
     5341 atcgcgtgcg gtacggtagt tctggaacag actctttgtt acttcgttca cgcggacgtc
     5401 agtgcactgg gcggaaagta tcgttgagac gaggagttga accggggttc tgtactcgag
     5461 ggcggtggtg gaacgagggt attccgcgtt gatgcggcgg tgaatctccg caacgcgttc
     5521 cttctcggcg cgcgcgctcc tttttcctgt agccatggct cctctcatcc tgcttccacc
     5581 tgattctgct gctggcacga agttccggga acgaagtgct ctagatgcca accagccaac
     5641 tgaacagcca gttcatgagc gagattacca cactcccttt t
//
LOCUS       DAOTNG010000207         5676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12019_length_5676_cov_4.641345, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000207 DAOTNG010000000
VERSION     DAOTNG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12019_length_5676_cov_4.641345"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="prmC"
                     /locus_tag="P5179_10045"
     CDS             complement(<1..700)
                     /gene="prmC"
                     /locus_tag="P5179_10045"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRS95600.1"
                     /translation="MSIETPTTLLDYLTRATEYFAGRGMENPRLNAERLLCGVLGCKR
                     IDLYVEFERVLNPGEIATYREYVRRRGRNEPLAYILGIAAFCGRDFVVTPSVLIPRPE
                     TELLVEHAIPFLKTATPTFIADIGTGSGCIGITFALGLPEAEVVMVDVSGEALEVARR
                     NAHEHGVHGRAHLVQGDLSHPLRAGTFHAVVANLPYVAAAEKNTLPPDVVEFEPHFAL
                     FGGETGLDLFECLLP"
     gene            complement(712..1749)
                     /locus_tag="P5179_10050"
     CDS             complement(712..1749)
                     /locus_tag="P5179_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95601.1"
                     /translation="MNPIKTYALARIGLLGNPSDGYFGKTISCAITNFSATVTLEEGE
                     GISIIPHPTSDPFSFESLANLRETAIRQGYEGGYRLIYATCKKFADYCAKREIVLPPK
                     GFTIRYDTTIPRQVGLAGSSAIITALTKGLRTLFGITESQFPLEIQPSFVLSVEEEEL
                     DIRAGLQDRVIQTYGGVVYMDFDRELLLHRGYGRYVQLDPRSVPPLFLAYVNRPKDSG
                     KAHSDVRARWSRGDEDVILAMREFADFAEQGREALAQRDWAKLGDLMNRNFDLRLRLF
                     GEKVIGDQNLEMVKIARSLGAPAKFPGSGGAVIGIWKDGDHFERLREAFASHGYAFTN
                     VQVENGPQYPV"
     gene            2042..2305
                     /locus_tag="P5179_10055"
     CDS             2042..2305
                     /locus_tag="P5179_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95602.1"
                     /translation="MRRAPWGLVLIGGLALTAVIGCTPKPSQEQLQALDRTCASATDA
                     ERALETKRRELSDVERQLGPKRQVLAERQRYLGEVRSNLQAMQ"
     gene            2317..2853
                     /locus_tag="P5179_10060"
     CDS             2317..2853
                     /locus_tag="P5179_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95603.1"
                     /translation="MIVRRAAVCVLACGTALFAGCAPSAQPGARPAAGALAERACLEA
                     AAAETSAARKMAAYRASQTDFSAQQARLVELQRIRSRIATGRMSDEELATWEVEPAPM
                     QAAPAETTQPVVPAVTAPGDSASVEQAAPTPEPQENVAPAVEEQAPPTSESETTPAPD
                     AETAPADSTAPGGEGSAE"
     gene            2930..3790
                     /locus_tag="P5179_10065"
     CDS             2930..3790
                     /locus_tag="P5179_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRS95604.1"
                     /translation="MTRTLYAVALGALLGLSAMAAAQTAPQMTMQQYEARLRECQNRQ
                     RAADSARAVVEQQIAQKRAELSSLDEQIAATNRQVLEAVAADSAAIRAYLGQLDGIIQ
                     QLQAMRQLPADQIVDARERGDLDRIAEQLAQLKTNRIAALPEAKAKIQTADQMIAELR
                     AVQRPVPEVRRDQYTVVRGDYLWKIAKKPEIYGDPYTWVRLYTANKDRIRNPDLIYPN
                     WVLGVPRNQAPGTYWVKRGDRMRTIAQEVYGDPSQWTKIYRANRDVIEAVSGGRRVIY
                     PNMILTIPQN"
     gene            3960..>4172
                     /locus_tag="P5179_10070"
     CDS             3960..>4172
                     /locus_tag="P5179_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95605.1"
                     /translation="MQHLFGPLDRNRKELEEEFHVSLVSRQGQLLVRGTEAEVAAAVK
                     ELDQRLRGDFVAPGPGTASPVALSKKR"
     assembly_gap    4173..4272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4273..4682
                     /locus_tag="P5179_10075"
     CDS             <4273..4682
                     /locus_tag="P5179_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRS95606.1"
                     /translation="HEMIPPDKLKNYLETHVIEIVPLAFMRGRTLNSAFVILDEAQNT
                     TTRQMKMFLTRLGADSKAVITGDITQIDLPAAVPSGLIDAKQILSGITGISFVSLSER
                     DVVRHRLVQLIIRAYDRVESSHGASNNANQQGV"
     gene            4695..>5676
                     /locus_tag="P5179_10080"
     CDS             4695..>5676
                     /locus_tag="P5179_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95607.1"
                     /translation="MRDHLQRLIRHDGVLVRLRRSLRGRLTEEYRKYLRILVGLVGIG
                     LFMALFPRERMNEYSSWREGMVAPREVIAPFSFNVRKNPLELETERRKARNSVPPVMR
                     MDPLVGLRNRRRVERFLDSLAGNPSPAAGTVSVSGIRPNRNAIQWLKASGGRNLGIAR
                     TVLVPVLERVYERGVVPVEDWQFLKEIVQRKVEAGAARGDSSDVVTVIRGDTLEYEQP
                     IGLMMNAEEARDELGQLITRVAMENVIQLSSDGLRALYAVAEAALEPNLAYDRTETAD
                     RQEQVMAAVPIYKRTVFKDERFIESHAVLTAEDIDEVGSLVSAQRARLRQA"
BASE COUNT         1186 a         1556 c         1726 g         1108 t
ORIGIN      
        1 caggcaacag gcactcgaac aagtcgagac cggtttcgcc cccgaaaagc gcaaagtgag
       61 gctcgaattc gacgacatcg ggaggcaatg tgttcttttc ggccgcggcc acgtagggaa
      121 gattcgccac cacggcgtgg aacgtcccgg cgcgaagcgg atggctcaga tcaccctgga
      181 ccaaatgcgc gcgaccgtgt acgccatgct catgcgcatt cctccgggcg acctccagcg
      241 cttcgccgga cacgtcaacc attacaactt ccgcctcagg cagtccgagt gcgaacgtga
      301 tgcctatgca cccgcttccg gttcctatgt cagcaatgaa cgtgggcgtc gccgttttca
      361 aaaaagggat cgcgtgttcg acgaggagct cagtttccgg gcggggtatc aataccgaag
      421 gggtgacgac aaaatcccgc ccgcagaacg ccgcgatgcc caggatgtac gccagtggtt
      481 cgttcctgcc gcgacggcgc acgtactcac ggtatgttgc gatttccccg ggattcagga
      541 ctctctcgaa ttcgacgtac agatcgatcc gtttgcagcc aagaacgccg cacagtagac
      601 gctccgcatt caagcgggga ttttccattc cgcgacccgc gaaatactcc gtcgcgcgag
      661 tgaggtaatc gagcagggtt gtcggggtct caatgctcat ggggttgcgg gtcagaccgg
      721 gtattgaggt ccattctcta cctggacgtt cgtgaacgcg taaccatggc tggcaaaggc
      781 ttcacggaga cgctcaaaat ggtcgccgtc cttccagatt ccgatcaccg cgccacctga
      841 accggggaac ttggcagggg ctcccagcga tcgtgctatc ttgaccatct ccaggttctg
      901 gtctccgatc accttttcgc cgaacagccg aaggcgtaag tcgaaattcc tgttcatcag
      961 gtcgcccagt ttcgcccaat cccgttgcgc aagcgcctct ctcccctgtt ccgcgaagtc
     1021 ggcgaattcg cgcatcgcca gaatcacgtc ttcgtcgccg cggctccacc gcgcccgcac
     1081 gtcgctgtgc gcttttcctg agtccttggg cctgttgaca taggcgagga agagaggggg
     1141 caccgatcgg ggatccagtt gcacgtatcg gccgtaccct cggtgcaaca gaagttcgcg
     1201 gtcaaagtcc atatagacca cgccgccgta cgtctggatt acccggtcct gcagcccggc
     1261 acggatatcc agttcttctt cttccacgct gaggacgaat gagggctgaa tttccaacgg
     1321 aaactgactc tcggtgatgc caaaaagggt acgaaggcct ttcgtaaggg cggttatgat
     1381 tgcactcgac ccggcgagcc cgacctgtcg aggtatcgtg gtgtcgtagc ggatagtgaa
     1441 ccctttcggc ggcagaacga tttctcgctt cgcgcagtaa tcggcgaatt ttttgcacgt
     1501 ggcatagata aggcgatacc cgccctcata tccctgccgg attgccgtct ctctcagatt
     1561 ggcaagcgac tcgaacgaaa acgggtcgga tgtggggtgg ggaataatgg agatcccttc
     1621 tccttcctcg agggtcaccg tggccgaaaa attggttatc gcgcaggaaa tcgtctttcc
     1681 gaaataacca tcggacgggt tcccgaggag cccgattctt gcaagtgcgt atgttttgat
     1741 gggattcaat gccgttcccc tttcgggcaa tgcgtgtcgc attttctcaa agtttggtgc
     1801 cttcgcctga ataggctcgt ggttgttttc tcgtggcccg tttcgtatca aatgatacgt
     1861 gtcgtatcaa agatacgcgg aacaaacgca ggtgcggcgc cgtagtacaa aaaaaagccc
     1921 cagcggttcg ggatggggat tgacgaatct cgcactcatg cgtatgttaa ggtcgagttg
     1981 gtttgctcac atcttcagaa tcgtgccgtt ttcggtatag tgccagacgg gaggaaatcg
     2041 tatgagacgt gcgccttggg gtttggtgct cattggcggc cttgcactta cggctgtcat
     2101 cggatgcacc ccgaagccca gtcaggagca gttgcaggcg ctggatcgga cctgcgcttc
     2161 agcgaccgat gcagagcgtg cgctggaaac aaagcgcagg gaattgtccg acgtggaacg
     2221 tcaactgggt ccgaagcgcc aggtgcttgc ggagcgtcag cggtacctcg gcgaagtccg
     2281 ttccaatctt caggcaatgc agtaagcgag tttgccatga tcgtccgtcg ggctgccgtg
     2341 tgcgttcttg cctgtggcac cgcgcttttt gcgggatgtg cgcctagtgc tcaaccgggt
     2401 gcgcgtccgg cggcaggtgc tctggccgaa cgggcgtgcc tggaagcagc agcggcggag
     2461 acctccgctg ccaggaagat ggcggcgtac cgtgcatcgc aaacggattt ctcggcacag
     2521 caggcaaggc tggtggaatt gcagagaatc cgttcgcgca tcgcgacagg cagaatgtcc
     2581 gacgaggaac tcgccacgtg ggaagtagag ccggcaccga tgcaggctgc tccggcggaa
     2641 accacgcagc ctgtcgtccc cgccgtgact gctccggggg attcggcatc tgttgaacag
     2701 gcagcgccga cgcccgagcc acaggaaaat gttgctccgg cggtggaaga gcaggcgccg
     2761 ccaacgagtg aaagtgaaac aacgcccgct cccgatgcgg aaacggctcc ggccgattcc
     2821 actgcgccgg gtggcgaagg cagtgcggaa tgacagtgaa atggttttca atcaagggat
     2881 tatgaccgtg aaatactcac cccgtgaaca cgggaagggg gtcacaggta tgactcgaac
     2941 gctttatgca gtggctttgg gcgcgttgct ggggttgtct gccatggctg cagcccagac
     3001 agcaccgcag atgacgatgc agcaatacga agcacgcctg cgcgaatgcc agaaccgtca
     3061 acgggccgcc gacagcgcgc gcgcggtggt ggagcagcag attgcccaga agcgggcgga
     3121 gttgtcatcg ctggacgagc agattgccgc caccaatcgg caagttttgg aagccgttgc
     3181 agcggacagc gcagcaatac gcgcctatct cgggcagctc gacgggataa tccagcaatt
     3241 gcaggcaatg cgccagctcc ccgcagatca gattgtggac gcgcgggaac ggggtgatct
     3301 cgaccgaatc gcagagcagt tggcgcagtt gaagaccaat cggattgccg ccctgccgga
     3361 agcgaaggcg aagatccaga cagcagatca gatgattgcg gaactgcgcg ctgtgcagcg
     3421 tcccgttccc gaggttcgcc gggatcagta cacggtggtt cgtggtgact acctgtggaa
     3481 aatcgccaaa aagcctgaaa tctatggcga tccctacacc tgggtccgtc tgtacacggc
     3541 caacaaagac agaattcgga acccggatct catctatccg aactgggtcc tcggcgtacc
     3601 ccgtaaccag gcccccggga cctactgggt gaaacgcggc gaccggatgc ggaccatcgc
     3661 ccaggaagtg tacggcgacc cgtctcagtg gacgaaaatc taccgtgcaa accgggacgt
     3721 catagaggcg gtgagcggcg ggcgcagggt gatttacccg aacatgattc tgacgattcc
     3781 gcagaactga cgaacgagac tcgcgccttg tgatccggcg gagatgatgt ggttcggatg
     3841 ccctctggct cctgcgggga gcccgggggc ttcctttttt ggggaggacg cgtgagaact
     3901 tcatgggagc caaagcacaa ccgttactga ctcagtcgat cgacatcgaa ggagcggaca
     3961 tgcagcacct tttcggtccg cttgacagga acaggaagga actggaagag gagttccatg
     4021 tctccctcgt ttcccgccag gggcagttgc tggtgcgagg aacagaagcg gaagtggccg
     4081 cggcagtcaa ggagctcgac cagcgcctga ggggggattt tgtagctccg ggacctggaa
     4141 ctgcctcccc ggtagcattg tcgaagaagc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nntgcacgag atgattccgc cagacaaact gaagaactac ctcgagacgc
     4321 acgtgatcga aatcgtgcca ctggcgttca tgcgggggcg aacgctgaac agtgcgtttg
     4381 taattctcga tgaagcgcag aacacgacga cacggcagat gaagatgttc ctgactcggc
     4441 tcggggcgga ctcaaaggca gtcataacgg gagacatcac gcagatcgat ctgcccgccg
     4501 ccgttcccag tggcctgatc gacgcgaaac agattctttc cggtatcacc ggaatatcct
     4561 tcgtgtcgct gtcagaacgt gacgttgttc gccacaggct tgttcagttg atcatccgtg
     4621 cgtatgatcg tgtggaaagt tcacacggcg ccagcaacaa cgcgaatcaa cagggcgtat
     4681 gaggggcccg cggaatgcga gaccaccttc aacggctcat ccgccacgat ggggtacttg
     4741 tgcgcctccg ccgatccttg cgggggcggt tgacggagga gtaccgcaag tatctgcgca
     4801 ttctggtcgg gctggtcggt atcggcctct tcatggcgct gtttccccgc gagcggatga
     4861 atgaatacag tagctggcgc gagggtatgg tggctccgcg ggaggtgatc gctcctttct
     4921 cgttcaatgt gagaaaaaac cccctcgaac tcgaaacaga aagaagaaaa gcgaggaatt
     4981 ccgtaccacc ggtcatgcgt atggaccctc tcgtcggatt gcggaaccgc cggcgtgttg
     5041 agcgttttct tgattcgctt gccggaaatc cctcgcccgc tgccggcacc gtttctgttt
     5101 ccgggattcg tccgaaccgg aacgcaatcc aatggttgaa agcgagcggc ggcagaaacc
     5161 tcggtattgc cagaaccgtg ctcgttcccg tcctggagcg ggtgtatgaa cgtggcgtgg
     5221 ttccggtgga ggactggcaa ttcctcaagg agatcgtcca acggaaggtt gaggccggtg
     5281 ccgccagagg agattcctcc gacgttgtaa cggtgatacg cggtgacacg cttgagtacg
     5341 agcaacccat cgggcttatg atgaacgccg aagaagcccg tgacgaactc ggccagttga
     5401 tcacccgtgt cgctatggag aacgtcattc agctttcatc agatggtctg cgcgcgttgt
     5461 acgcggtggc ggaagcggcg ttggaaccga atctggcata tgacagaacg gagaccgctg
     5521 acagacagga acaggtgatg gcggcggttc cgatttacaa gcgaacggtg ttcaaagatg
     5581 agcggttcat agagagccac gccgttctga ccgcggaaga cattgatgaa gtaggctccc
     5641 tggtttcagc gcaacgggcg cggctacggc aggcgt
//
LOCUS       DAOTNG010000208         5645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12097_length_5645_cov_5.598032, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000208 DAOTNG010000000
VERSION     DAOTNG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12097_length_5645_cov_5.598032"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1301)
                     /gene="mraY"
                     /locus_tag="P5179_10085"
     CDS             complement(150..1301)
                     /gene="mraY"
                     /locus_tag="P5179_10085"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRS95608.1"
                     /translation="MLYHLASEAFQWYRQAPVSFPVLGSDVLLLTRFLMYLSVRAILA
                     ILTSFVVTMLVGRRLIRFLHAHHVRDTVKDFGTISATDKRGTPTMGGLIIITSLVVPV
                     LLWCDLTNRFVQIALAATAWFGALGVVDDYLKVRPYRGRIRGLGEAQKLALQGLFGLG
                     LGIAYLTPAISPVPQEVAGSFYVPFYKYAVLHDIGWWYLPFIVFVTLLISNSVNLTDG
                     MDGLAIMPANFAVLVYGVIAYAMGNLVYAEYLRYDYLHGSGELLVFCAALFGAGLGFL
                     WYNAYPAEIIMGDTGSMALGGILASLAIMLKQEVLFLIVGGVFVAETLSSFIQQKIGE
                     NFLGRRIFYRAPLHHQMEYRGLAETKVVIRLWIIAGLLAMVGLASMKLR"
     gene            complement(1304..3403)
                     /locus_tag="P5179_10090"
     CDS             complement(1304..3403)
                     /locus_tag="P5179_10090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS95609.1"
                     /translation="MGHRFILTGGGTGGHVTPAIALGEELRAREPDAQFLYVGTRTGK
                     EAEIVPRYGFPIAYVQSHQWSFRRKWHVLRFIWRIACGTIKACHLIIKYRPDAVIGTG
                     GYVAAPVVFANVLLRRIRLSRALTIIHEQNTVPGKLNALVGKVADIVLLSFPTASARF
                     PRAVCVGYPVRPEIAPADRTEAKRRLGIPQETTVVLAFGGSMGARTINRAVVDALPFL
                     RDRKDLHIVHGTGRKLSSYDPVADCESRMQRNGLTYESLSGWYEVHEFIDRIGEYYAA
                     ADVVVCRSGAGTLAELCACGRAALLIPKANLPGDHQVLNALEMARRGAADILFEDAVT
                     TNGAPEEYVAGEELARKIVELLEDKPRLGKMESNALASARPEATKVSVGVILSLLEGR
                     SPDGLLLEFAAREPVVGDAHLDTRFGRVAALSGEALRAYAETAVSSISRSLNCASGTP
                     SDQEDALVRALDADEMVAYLRYRAATHLTSKNWRMRNAGVKMVGILRQRDKLPVLAGF
                     VKDRAPASRMRRLFGGDFETNGFIRRNSMASLIRLGVASPLMREVILCGLEDPYFEVR
                     SQAARAVQAFRASLENDQEIVERLLNCAKDKYFEVAVEAVKAMGSVASAETALEVFRS
                     LSQHENWRVRQAVVEACCALLDRGQDVPKAFLSEILAGMMVTSTGFCPSFSLKLAVAK
                     LGERIREPDSTAGDRGK"
     gene            complement(3456..5186)
                     /gene="murJ"
                     /locus_tag="P5179_10095"
     CDS             complement(3456..5186)
                     /gene="murJ"
                     /locus_tag="P5179_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRS95610.1"
                     /translation="MGILLLMQISLKDGTVSEAMAEARNANVSPVKDHPFPSPQRKIF
                     GYLSFQVLGTLVSRAAGFVRELVTAVVFGAGASADAFIAALTIPTLFRDVLGEDVVER
                     SFMPGIREHLARKNHADAWRLASVSLNWMLVALVLGMGVLWVAAPWLVDLVAAGIHQR
                     SVSEGTVADVIAMTRILIPFLVFIALGAYVGGVLYYGFDFHLVFSLAPTMLSVGVIAI
                     VLFSSQDIGIYALAWGFTVGAVLQFAVQVPFLGSQKIRSTNPEYTLSLRLNPAERSRI
                     SRETAHVALQSLLTKTTEIVDRRVASFLVEGSISSLWFAARLVQLPFAIIGIAIGRAV
                     APYLSEKVGTGDLREFKNAVLIGYRYNLVLVLPVIGLFTVLAHPFVRLIYERGNFGAH
                     DTSMTASAFWAYALGLLGLSLYTLGTRICSALEHNRVATYTAVLGAGLNVWLNYVLSA
                     TFLRHAGLALATSIAFSVNAALLLMWVHRYLAQCGQGFRTRELLVPFLRVLANTAGAV
                     LAAWVVLAFFVPESWFVTRSFAASVGAFCVPFAGGFLVYFVLAVINPVAEIVPVKRWM
                     KRSVIRFGNR"
BASE COUNT         1367 a         1701 c         1538 g         1039 t
ORIGIN      
        1 ggctccccag cggaaacgga tggtcaaaac gagctgctga atcttgtact cgtggatctc
       61 atttggtcca tgcgcactcg caaacaaacg agcgctgaca tgaccggacg cacgaggagg
      121 ggagatttca cgcgggaaga aaggggaaat cacctcagct tcatggaggc aagcccgacc
      181 atcgcaagca gacctgcaat gatccagagg cggatcacca ctttcgtctc ggccaggccc
      241 cggtattcca tctggtggtg cagcggcgca cgataaaaga tgcgccttcc caggaagttt
      301 tccccaatct tctgctgaat gaaggaagac agagtctctg caacgaacac accacccacg
      361 atcagaaaaa gaacctcttg cttcaacatg atggccagag acgcaagaat acccccgagt
      421 gccatcgagc cggtgtcgcc catgatgatc tcggcgggat atgcgttgta ccagagaaac
      481 ccgagacctg ctccgaacag cgctgcgcag aataccagca actccccgga accgtgcagg
      541 taatcgtacc tcaagtactc cgcatacact aagttaccca ttgcgtatgc aatgaccccg
      601 taaaccagaa ccgcgaagtt ggcgggcatg atcgcgaggc cgtccatgcc gtcggtgaga
      661 tttacgctgt tcgaaatgag cagggttacg aacacgataa acggcagata ccaccagcca
      721 atgtcgtgca ggaccgcata tttgtaaaac gggacgtaga agctgcctgc tacctcctga
      781 ggtacgggag aaatcgccgg tgtcagatat gcgatgccca accccagacc aaacaatccc
      841 tgaagcgcga gcttctgagc ctcgccgagt ccgcgaatac gtccacgata cggtcgtact
      901 ttcaggtagt catcgacgac cccgagcgct ccgaaccatg cggttgcggc gagtgcaatc
      961 tgcacaaacc ggttggtcaa gtcgcaccac agcaataccg ggacaacgag ggaagtgatg
     1021 atgatgaggc cgcccatcgt cggcgttccg cgcttgtccg tcgctgaaat ggtcccgaaa
     1081 tctttcaccg tgtcacgcac atggtgcgca tgcagaaatc ggatcagcct tcgtccaacg
     1141 agcatggtga cgacaaacga cgtcagtatc gcgaggatcg cacgaacgga gaggtacatg
     1201 aggaagcggg tcagcaatag tacgtcagat ccaagtaccg gaaaactgac cggcgcctgc
     1261 ctgtaccact ggaatgcctc cgacgcaagg tgataaagca ttactatttc cctcggtcgc
     1321 ccgctgtgct gtcaggttcg cggattcgtt ctccgagctt cgccaccgcg agtttcagcg
     1381 aaaacgatgg gcagaacccg gtacttgtca ccatcatccc cgccagtatt tcgctcaaaa
     1441 atgccttcgg tacgtcctgc cctctatcca agagggcgca gcacgcttcc accaccgcct
     1501 gccgcactcg ccagttctcg tgctgggaca gagagcggaa cacctccagg gccgtttctg
     1561 cagaagcaac ggagcccata gccttcactg cttccactgc aacttcaaag tatttgtcct
     1621 tcgcgcaatt cagcaacctc tcgacaattt cctgatcatt ttcgagactc gcacggaacg
     1681 cctgtaccgc gcgcgcggct tgcgaacgaa cctcaaaata cggatcctcc agtccacaca
     1741 ggatgacttc gcgcataaga ggagacgcca ctccgagcct gatcaggctt gccattgagt
     1801 tgcgacgaat gaacccgttc gtttcgaaat cgccgccgaa gagtcggcgc atgcgcgagg
     1861 cgggcgctcg atccttcacg aatccggcca gaaccggaag tttgtcacgc tgccggagga
     1921 tccccaccat ttttacaccg gcattccgca ttcgccagtt cttcgatgtc aggtgtgtgg
     1981 cggcccggta tctgagatac gcgaccattt cgtccgcatc cagggcacgg acaagagcgt
     2041 cttcctggtc tgatggcgta ccggacgcgc agttcaggct gcgagagatg ctggatactg
     2101 ccgtctccgc atacgcgcgg agcgcctctc ctgagagcgc agcgacgcgt ccaaacctcg
     2161 tgtcgaggtg cgcgtctccc accaccggtt cccgcgcggc gaattccaac aacaaaccgt
     2221 ccggagacct gccttcaagc aacgaaagaa taacgccgac tgagaccttg gttgcttcag
     2281 gccgtgcgct cgccagagca ttcgactcca ttttgcccag tctcggcttg tcttccagca
     2341 actccacaat cttgcgggcc agttcctcgc cggccacgta ttcctccggt gcaccgttcg
     2401 tcgtaaccgc atcttcgaaa agaatatcgg ctgcgccgcg ccgcgccatc tccagagcat
     2461 tcagtacctg gtggtctccg ggcagattcg ccttcgggat gagaagagcc gcgcgcccgc
     2521 aggcgcacag ttctgccagc gtcccggcac cagaacggca gacaacgaca tccgcggcgg
     2581 cgtagtattc gccgatccgg tcaataaact cgtgtacctc gtaccatcct gaaagcgact
     2641 cgtacgtaag tccgttccgt tgcatccgcg actcacaatc ggcaaccggg tcatacgacg
     2701 agagctttcg cccggttccg tggacgatgt ggaggtcctt gcggtcccgg aggaagggca
     2761 acgcgtccac gactgcgcgg ttgatcgttc tcgctcccat cgagccgcca aaagcgagta
     2821 cgactgtcgt ctcctgcggg attccgagtc ggcgttttgc ttccgtccga tccgcgggcg
     2881 cgatttctgg ccggaccggg taaccaacgc acaccgcccg cgggaaacga gcggaagcag
     2941 tgggaaagct cagaagcaca atgtccgcaa ctttgcccac cagggcgttc agttttcctg
     3001 ggacggtatt ctgttcgtga atgattgtca gggcacggga gagtcgaatt cggcggagaa
     3061 gaacgttcgc aaaaaccaca ggcgcggcaa cataccctcc cgtgccgatc accgcgtccg
     3121 gtcggtattt gatgatgaga tggcacgctt tgatggttcc acacgcgatg cgccagataa
     3181 agcgcaaaac gtgccatttc cgacgaaagc tccactggtg gctttgtaca tacgcgatcg
     3241 ggaacccgta ccggggcacg atctccgctt ccttgccggt ccgcgtccca acgtaaagaa
     3301 actgcgcgtc aggttcgcgg gcacgcagtt cttcaccaag ggctatggcc ggtgtaacat
     3361 ggccgcccgt gccgcctccc gtcagtatga accgatgtcc cattcagaac ctgcgtggcg
     3421 aaagtgcccc ttgatacgga gtcctgcgtt ccctttcaac ggtttccaaa gcgaatcacg
     3481 gagcgtttca tccagcgttt cacgggtact atctccgcaa ccggattaat caccgcaaga
     3541 acgaaataca caaggaagcc cccggcaaag ggcacacaaa aagcgcccac ggaagcggca
     3601 aaactccgcg ttacgaacca ggattcaggt acaaaaaacg ccaaaaccac ccaggctgca
     3661 agcactgccc ctgcggtgtt ggccaggact cgcaggaaag gcacgagcag ttcccgagtg
     3721 cggaagcctt gcccacactg agccagatat cggtgtaccc acatgagaag taaggcagca
     3781 ttgacgctga aggcaatgga ggtggcaaga gcaagcccgg cgtgacggag gaaggtggca
     3841 gaaagaacgt aattcagcca tacattcaga cctgcgccga gcaccgcggt gtacgttgcc
     3901 acgcgattgt gttccagcgc agagcagatg cgcgttccaa gcgtgtaaag cgagagcccg
     3961 agcaacccca aggcatatgc ccagaaagca cttgccgtca tcgacgtgtc gtgtgcgccg
     4021 aaattgcccc gctcataaat cagccggaca aaagggtgtg ccagcacggt gaaaagcccg
     4081 ataacgggga gcaccagaac gagattgtag cggtatccga taagaaccgc attcttgaac
     4141 tccctcaggt cgccagttcc cactttctca gacagatacg gagcaactgc gcggcctatt
     4201 gctatcccga ttatcgcaaa aggaagctga acaagccgtg cggcgaacca aaggcttgag
     4261 atacttccct cgacaagaaa tgacgcgact ctgcgatcga ctatttctgt ggttttcgtg
     4321 agcagcgact gaagtgcgac gtgggccgtt tcccgagata ttcgcgaccg ctccgcaggg
     4381 ttcagtcgca aactcagagt gtactctgga ttcgtggatc ggattttctg tgagccaaga
     4441 aacggcacct gcacggcaaa ctgcagaacc gctcccactg tgaatcccca cgcgagagcg
     4501 taaatcccga tatcctgcga gctgaacaac acaatcgcga tcacacctac gctcagcatc
     4561 gtgggggcga ggctgaagac caggtgaaaa tcgaacccat agtagagcac gccaccgaca
     4621 taagcaccaa gcgcaatgaa caccagaaaa gggatcaata tgcgtgtcat tgcgatgacg
     4681 tcggctaccg tcccctcgct gaccgaccgc tggtggatac ctgccgcaac gagatcaacc
     4741 agccacggcg cggccaccca cagaacgccc attccaagaa cgagcgctac cagcatccag
     4801 ttgagtgaga cggaagccag gcgccaggcg tccgcatggt tttttcgcgc aagatgctcg
     4861 cggatacccg gcatgaagga gcgttcgacc acgtcctcac caagcacgtc ccgaaaaagc
     4921 gtcggtatgg tgagtgctgc aatgaacgca tctgcggaag cgcccgcgcc gaaaacaacc
     4981 gcggtgacaa gctctcgcac gaaaccggcg gcgcgactta cgagtgttcc caaaacctgg
     5041 aaggacaggt acccgaagat cttgcgctga ggagacggga atgggtgatc tttgacaggg
     5101 gagacatttg catttcgcgc ttcagccatt gcctcagaaa ccgtcccgtc tttgagcgaa
     5161 atctgcatca agagtaatat gcccacggca cggggatcgc tgcaaggagg gaacaaaccg
     5221 ctttccgggc agattcggca ggccccacgt accgacgagt cgaagaaccg tcacatctcc
     5281 aattgaagcg ctggcgaggc tgcgagggcc gttccaccag attcgtcagt gactcctcaa
     5341 tcaaaagaac ggtggaacga actcggcctg acgggtgttt ttaacggtac ggagattgcg
     5401 tgtactgcat gcgactcaac ctgggtttga ttccgtcaag acctgaaaca aaagcgctcc
     5461 aaaggacttt cgacatgaaa gagattgaag tgatgcagac ccccgagcca aggccccttt
     5521 caagaccacg ccccaagcaa cctgcggagc aacccgcaaa ggcttcggac atcatcgacg
     5581 aagcccacat cgagggattg ttgtcttcat ccaggtctgc cgacaaatct gagatccgag
     5641 agata
//
LOCUS       DAOTNG010000209         5613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12190_length_5613_cov_4.855164, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000209 DAOTNG010000000
VERSION     DAOTNG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12190_length_5613_cov_4.855164"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="P5179_10100"
     CDS             complement(<1..556)
                     /locus_tag="P5179_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95611.1"
                     /translation="MDNTVIVVILALAVIILFFFRMKKTTGLNRNLMPRRLIEKEEAE
                     EQAAISAKEPDWTLKDLTTGGRIHFSLPGGLEESYTVLRRDRIEWPDEQIEYDLLMRG
                     DDPNHEVWLHWWTIGRATHAWLVEVIEKAPAELGFDVTQLEAVRSGGSLKARYRDQDY
                     TLAKAGSLLALENGLRPGKEFARWD"
     gene            839..1342
                     /gene="tpx"
                     /locus_tag="P5179_10105"
     CDS             839..1342
                     /gene="tpx"
                     /locus_tag="P5179_10105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRS95612.1"
                     /translation="MGSVTFKGNPVTLVGNEVVVGDSAPEFALAANDLSTVNLAATAG
                     KVRIISVVPSLDTPTCDAQTRRFNQEAAALGPDVAILTVSMDLPFAQKRWCAAAGITA
                     VQTLSDYKDHSFGVAYGVRIKELGLLARSVFVVDRAGKVTYREIVSEVTHEPDYARAI
                     EAARKAL"
     gene            1748..2314
                     /locus_tag="P5179_10110"
     CDS             1748..2314
                     /locus_tag="P5179_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95613.1"
                     /translation="MIGPFVVRFLAIMGIFIPGVNQQVSLLDPFVELRGDRIAVSTAL
                     QKPLSTDLLKIIDSGTPVTLTYVCRLRTSTDNPAAAPDTTVSHRVVKDLASGTYEIDT
                     GTRKILTSRITEYSLLFRLETVPLWNINVLSNSGFYYVEVSARFEPIVIHATGERYDL
                     MALWSYRPPTARSKLFNRFIVERRRVEP"
     gene            2311..3516
                     /locus_tag="P5179_10115"
     CDS             2311..3516
                     /locus_tag="P5179_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS95614.1"
                     /translation="MSLRARFILSAMILLLAGMLPMGWFAMRSIDKTIRVWHDAQVGA
                     ALQQSLTHVENEAVRRQVNEALIRYNQLGALQRPMQWQLVIVGLTLAFAIVVLASLAA
                     WFLAVQLTRPLNSVAKAAEDIARGDLSHTVPPARIPEIAALVDTFNAMVVSLRQSRAA
                     LARAERRAAWQDIARAIAHEIKNPLTPMRLTTQHLRERFQENRPRFEETFIRSTDMIL
                     TEIDRLERLANAFSTFAKMPAPVMEPLDLRVLIGQTTDLFPAERDGGRLIVHLPETPV
                     TVSGDLEQLKQALLNLVKNAFEAPGEGERRVELSLSSTATDAIVEVKDDGTGIPPDML
                     ERLFQPYITTKSGGSGIGLAIVDRVITDHQGRVTAQNMEQGGALFRVWLPLQNPGFIR
                     EDTYAHPGY"
     gene            3497..4888
                     /locus_tag="P5179_10120"
     CDS             3497..4888
                     /locus_tag="P5179_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS95615.1"
                     /translation="MRILVIDDEPNIRRSLRDILEDEGYEVDEAGDGETGLRLLERQP
                     ADIVLLDVKLPKADGVTILGDVRARLPHTEIIMISGHSDIETAVDALRKGAYDFLEKP
                     LSLPKVRLAVAHAAEKISLRKRAREQAAARFQPLIGTSAAMSAVRAMISRVAPTTSSV
                     LIRGESGTGKELAAQQIHVQSNVSDGPFVQTNCAAIPHDLIESELFGHEKGAFTGAAA
                     RRLGKFEQANNGTLFLDEIGDMDIAVQAKVLRALQNGEFQRVGGTETLFAKVRVVAAT
                     NKNLEKEVEEGRFREDLFFRLNVVPIFMPPLREHPEDIPELARHFLARFCMENGREQI
                     RITDEALERLKTFSFRGNVRELKNLVERLAIFSTGNEITLATVLAQAHPVLTGQNTHF
                     LRSRPLAEAKNELEKTYIETQLNLYGWDIVRTAEALDILPNNLHRKITQLGIQRPHRK
                     HGTKDEEEAGDSE"
     gene            complement(4971..5210)
                     /locus_tag="P5179_10125"
     CDS             complement(4971..5210)
                     /locus_tag="P5179_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95616.1"
                     /translation="MYGARCPDPHTRRPVIPAKAGIHVDAIGSSSAQYPTTLRKKEPA
                     RGSVFLRGFSIPACAGMTRADCFGGLAHEQAAETV"
     gene            5400..>5613
                     /locus_tag="P5179_10130"
     CDS             5400..>5613
                     /locus_tag="P5179_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95617.1"
                     /translation="MRHPSHYRVIWHEVGHALQYMDFPIVPENVLKKAVLPITRTQVD
                     CLSWLAHTYLGGPYDMPRMWRDYLDPS"
BASE COUNT         1144 a         1770 c         1652 g         1047 t
ORIGIN      
        1 agtcccaacg agcgaactcc ttgcccggtc ggagcccgtt ttccagcgcc agcaatgacc
       61 cggccttggc aagcgtgtag tcctggtcac ggtaccgggc cttcaacgat cccccggacc
      121 gcacggcttc gagttgcgtc acatcgaaac caagttcggc gggagccttc tcgataacct
      181 ctaccagcca cgcatgagtg gctctgccga tggtccacca gtggagccag acctcgtggt
      241 ttgggtcgtc gccacgcatc agaaggtcgt attcgatctg ctcgtcaggc cattcgattc
      301 ggtcccggcg cagaacagta tagctttcct ccagtccgcc gggaagcgaa aagtggatcc
      361 tgcctccggt cgtcaggtcc ttcagggtcc aatccggctc tttggcgctg attgccgcct
      421 gctcctccgc ctcttccttc tcaatgaggc ggcgcggcat gaggttccgg ttcaacccgg
      481 tcgtcttctt catgcggaaa aagaagagga tgataaccgc cagcgccaga ataacgacga
      541 tgaccgtgtt gtccatcgta tgccctcgtg gaaccaattg taatgatcgc ccggttcagt
      601 atggtgtaaa cgtaacccgg acgccgcggc accgcaaatg ggaatcgcca cggctgactg
      661 taggcacaca atcaggggag gaaccacagt ggcgtgcgca ccgtggaaat cctggaccat
      721 cgcgggcctc tgcgttctgg cgggagtaac cgccggaatc atgtgggccc gatcagactg
      781 ccgcagggca ggcaacactg cacgaaattc caccccgtct cgattgggag aagaacgaat
      841 gggttctgta accttcaagg gaaaccccgt tacgctggtg gggaatgagg ttgtcgtggg
      901 cgacagcgcg ccggagttcg ctcttgccgc aaacgatctc tccacggtca acctcgccgc
      961 aacggccggc aaagtgcgca ttatcagcgt ggttccgagc ctcgacacgc ccacatgcga
     1021 cgcccagacg cgccggttca atcaggaagc cgccgcgctc gggccggatg tcgcaatcct
     1081 gaccgtcagt atggacctgc ccttcgcgca gaaacgctgg tgcgccgccg cggggatcac
     1141 cgcagtgcag acgctttcgg attacaaaga ccactccttc ggcgtcgcat acggtgtgcg
     1201 catcaaggaa ctcggtctcc tcgcccgcag cgtgttcgtc gtggacaggg ccggcaaagt
     1261 gacgtaccgg gagatcgtca gcgaggtaac gcacgaaccc gattacgcaa gggcgatcga
     1321 ggcggcccgc aaggcgttgt aacagcgcaa ccgttgcgat cgccggtctc agaaacagac
     1381 agaaccccgt tgccggtcat cgagtgcggc gccgtccatg caatggacgg cgccgtgtat
     1441 cgagatgacc cgagttcccg accggtgggg cttcggccac gaactgcgga gcgactcacc
     1501 ggtggggctt tcccgccgta ggcgggacgg cattcttgcc tgccctgcgg catagccgac
     1561 acctgctcac tcccgcatcc gaaccggccg tatgctttat cagaacactc cgtccgccgc
     1621 cgccgcaatg ccctccccga atatgaatcc ccctccgcga gtagtacatt attgacaacc
     1681 tcttaggaaa gcgtgaacca ttgcgggcgt gtattgacgc gcgtaccggc tatcccgcgg
     1741 ggaaagcgtg atcggacctt ttgtcgttcg gtttctggcg atcatgggga tattcatccc
     1801 cggtgtgaac cagcaggtct ccctcctcga tccgttcgtc gaacttcgcg gcgatcgcat
     1861 cgccgtgtct accgcccttc agaaaccact ttcgaccgat ctgctgaaaa tcattgacag
     1921 cggcacgccc gtcaccctga cctatgtctg ccgcctgcgt acgtcaaccg acaaccctgc
     1981 cgctgcgccg gatacgacgg tttcccatcg cgtggtaaag gacctcgcgt ccggaacgta
     2041 tgaaatagac accggcacgc gcaaaatcct cacctcgcgc atcacggagt attcgctcct
     2101 gtttcgtctc gaaaccgtgc cgctctggaa catcaacgtg ctttccaatt ccggctttta
     2161 ctatgtggag gtttcagcga ggttcgaacc gattgtcatt catgccacgg gggagcggta
     2221 cgacctcatg gcgctctgga gctatcgtcc cccgacagcg cgcagcaaac tgttcaatcg
     2281 cttcatcgtg gaacggcggc gggtagagcc atgagtctcc gcgcgcgatt cattctctcg
     2341 gcgatgatcc tcctgctcgc cggcatgttg ccgatgggct ggttcgccat gcggtccatc
     2401 gacaaaacca tccgcgtctg gcacgatgcg caggtcggag cggcgttgca gcaaagcctc
     2461 acacacgtgg agaatgaagc ggtcaggcgc caggtgaacg aggcgctcat ccgctacaac
     2521 cagctcgggg cgttgcagcg gcccatgcag tggcaactcg tcattgtcgg gttgacgctg
     2581 gcattcgcca ttgtcgttct ggcatctctg gccgcatggt ttctcgccgt tcagcttacc
     2641 cggccgctca actcggttgc gaaggccgcc gaggatattg cccgcggcga tctttcgcac
     2701 acggttcccc cggcgcgaat tcccgaaatc gccgcgctgg tggatacgtt caacgcgatg
     2761 gtggtcagtc tgcggcaatc gcgcgccgcg ctggcacggg cggaacggcg agccgcgtgg
     2821 caggatatcg cccgcgccat cgcccacgag atcaagaacc cccttacccc catgcgtctg
     2881 accacccagc atctgcgcga acggttccag gaaaatcgcc cgcgtttcga ggagacgttc
     2941 attcgctcca cggacatgat cctgacggaa attgaccggc tcgagcggtt ggccaacgca
     3001 ttttcaacct tcgcgaagat gccggctccc gtcatggagc cgctggacct gagagttctg
     3061 atcggtcaga caacggactt gttccccgcg gagcgcgacg gtggtcggct catcgtccac
     3121 cttccggaga ccccggtcac tgtatcaggc gatctggagc aactgaagca ggcgttgctg
     3181 aatcttgtga aaaatgcctt cgaggccccg ggcgaggggg agcgccgggt ggagctcagc
     3241 ctctcctcta ccgcgacgga tgcgattgtg gaagtgaagg acgacggaac gggcattccg
     3301 ccggacatgc tggaacggtt gttccagcct tacatcacga caaaatcggg agggtcaggg
     3361 atcgggctcg ccattgtcga tcgcgtaatc actgaccacc aggggcgtgt caccgcgcag
     3421 aacatggaac agggcggcgc gttgttccgc gtatggctgc cgctccagaa cccggggttt
     3481 attcgagagg atacgtatgc gcatcctggt tattgacgac gaaccgaaca tccgacgatc
     3541 gctccgcgat atcctggagg acgaagggta cgaggtggac gaagccgggg acggcgaaac
     3601 cggactgcgt cttctggaac gccagccggc cgatatcgtg ctccttgacg tgaagcttcc
     3661 gaaggctgac ggcgtgacga ttcttggcga tgtcagagca cgcctgccgc acacggaaat
     3721 catcatgata tccgggcact cggatatcga aacggcagtt gatgcactgc gcaaaggcgc
     3781 ctacgatttc ctggaaaaac cgctcagcct gccgaaggtg cgccttgctg tcgcccacgc
     3841 cgcggaaaag atctcactcc gaaaacgtgc ccgcgagcag gcggcagccc gattccagcc
     3901 gctcatcggt accagtgctg caatgagcgc ggtgcgcgcg atgatcagcc gcgttgcgcc
     3961 gacaacttcc agcgtactca tccggggaga aagcggcacc ggcaaagaac ttgccgccca
     4021 gcagattcac gtccagagca atgtctcgga tgggccgttc gtacaaacca actgcgccgc
     4081 cattccccac gatctcatcg aaagtgagct gttcgggcat gagaaagggg catttacggg
     4141 tgccgcggcg cgccggcttg gcaaattcga gcaggcgaac aacgggacgc tgttccttga
     4201 cgaaatcggc gacatggata tcgccgtgca ggcgaaggtg ttgcgcgcac tgcagaacgg
     4261 cgaatttcag agggttgggg gcacggaaac attgttcgcc aaggtccgcg tcgtcgctgc
     4321 gaccaacaaa aacctggaga aagaagtcga agaaggtcgc ttccgcgagg acctgttttt
     4381 ccgcctcaac gtggtgccga tcttcatgcc tcccctgcgg gagcatccgg aagacatccc
     4441 ggagctggcg cggcacttcc tcgcccgctt ctgcatggaa aacggtcgcg agcagatccg
     4501 catcaccgac gaggccctgg agcgcttgaa aaccttctct ttccggggaa atgtgcgcga
     4561 actcaagaac ctcgtggaac gactggcgat cttctccacc ggcaacgaaa tcacccttgc
     4621 taccgttctt gcgcaggcac acccggtcct gaccggccag aacacgcact tcctccgctc
     4681 acgacctctc gcggaggcga aaaacgagct ggagaagacg tacatcgaga cgcagttgaa
     4741 cctttacggg tgggacatcg tccgcacggc cgaagcactc gacatcctgc cgaacaacct
     4801 ccaccggaag atcacgcagc tcggtatcca gcgcccgcac cgcaaacacg gcacaaagga
     4861 cgaggaagag gcaggcgact cagaatgacg gcacgcgggc gggactccag gcttcccgta
     4921 gcacgggcat cctgcccgtg ccagatttcc gcgggcggtc atggtgaatc ttacacggtt
     4981 tctgcggcct gctcatgcgc aagtccgccg aagcaatctg cacgcgtcat tcccgcgcag
     5041 gcgggaatcg agaaaccccg caaaaacaca ctcccgcgtg ccggttcctt cttgcgaagc
     5101 gttgtcgggt actgcgccga tgaagaaccg atggcgtcaa cgtggattcc cgccttcgcg
     5161 ggaatgacgg gacgccgggt gtgcgggtcg gggcaccgtg cgccgtacac acaccgacct
     5221 gccggtgcag ccacaccccg ctgcgccaaa aggagagagt gcgcggccct tcccgaggcg
     5281 gaaacggctc agattggctc gggtacctta ttccgaactt gaccgtcatt ctgagcgaag
     5341 cgaagaatct gttcctgaac tagttcggga aataacctcg tgatgcactg gagaacaaca
     5401 tgcgtcaccc atcgcattac cgcgtcattt ggcacgaagt cggccacgcg cttcagtaca
     5461 tggactttcc gatcgtcccg gagaacgttc tgaagaaggc agtgctccca atcacacgca
     5521 cgcaggtaga ttgcctctcc tggctcgcgc acacctacct cggcgggccg tacgatatgc
     5581 cccgcatgtg gcgcgattat ctcgatccct cac
//
LOCUS       DAOTNG010000210         5597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12230_length_5597_cov_5.302418, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000210 DAOTNG010000000
VERSION     DAOTNG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12230_length_5597_cov_5.302418"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5179_10135"
     CDS             complement(<1..429)
                     /locus_tag="P5179_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HRS95618.1"
                     /translation="MSVPTSLAVLFVLLCLSAFFSASETALVAMTKVRVRRALDHYGR
                     KAKPLLQWDRHSDRLLTAIVIGNNVVNIGAASVTAMVLSYYIADPETAVWVNTALMTT
                     VILIFGEILPKQLGKSHSESTSLVVIRPVIALSSILWPLVW"
     gene            complement(455..1942)
                     /locus_tag="P5179_10140"
     CDS             complement(455..1942)
                     /locus_tag="P5179_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRS95619.1"
                     /translation="MEPPTESRRHILVVAHRGAHQSCPENTVESIEQAIALGVDLIEV
                     DIRVTGDGHFVCLHNSTVDARTNGQGRVRDLTLTELKALDAGSKFSSAFAGASIPTVD
                     EVFARMRGHVGAYVDCKDAPPDSVADLLARHGLVEVSVVYADPAELREMIRREPRIRP
                     LPEYPGDVSAWHALADSIPTNHVAISSLANLSSESVRACHSRGAKVIVDIMAEDAPEG
                     WQRALEMGVDGLQTDRPAELLAFLRAKGLRRENVPPRVIEVIAHRGAHLNQPENTVPA
                     IEEAIAVGADWVEIDPAYTADRKLVILHDQKVNRTTDGTGKVTDLTLAEVRALNVKNP
                     DGSLAAGVRIPTLDEALAAMRGRVRIYLDAGGIDAHDLVEAMRRQGVSEQTAVYSDGR
                     TLAELRALEPAAFRLLPRPPEDLSTLPEIMERVQPAIFCASLRDITPAKVAACHRAGA
                     RVFCNVLSKDTPDGWGQAIECGADALETDKPAELLAFLRARSLHP"
     gene            complement(2053..3042)
                     /gene="nadA"
                     /locus_tag="P5179_10145"
     CDS             complement(2053..3042)
                     /gene="nadA"
                     /locus_tag="P5179_10145"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRS95620.1"
                     /translation="MAVDTIVREDGVMLEPAPDPREMRKFADQNGILILAHSYQDGHI
                     QDAAHFVGDSLELARKAVEVKASRICFCGVHFMAETAKILNPESHVFMPDLEAGCSLA
                     EGCDAQDLARYQTHLKEKLGRDDLVTICYVNCTAAVKALSDVVCTSGNALEVVNSVPR
                     GTPILFVPDVHLGGWVKENTDREIYLWNATCIVHELFSVDTLRTLKKEHPDAQILAHP
                     ECPKNIRDEADFVRGTAGMLRIVTANRGKTYIVATEGNMVHRFKTEAPENTYVAAPGP
                     SCACNLCPHMQKNTPEKLWYSLRTGRYEVFVDDAVASNARKALERMLAVRVTR"
     gene            complement(3251..4318)
                     /locus_tag="P5179_10150"
     CDS             complement(3251..4318)
                     /locus_tag="P5179_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95621.1"
                     /translation="MPRETMTPRERWEAVLRRQKPDRIPMDYWATPEATRKLQAHLGV
                     NSTEELHAKLHIDATLSVGGNYVGPPAPEGEDVFGIRYRTVEYATGVYTEAVHNPLAR
                     YRSVEEIEANYRWPQADWWDYSHIPDQIRGNEHRPIAGGGSEPFLRYCQLRGLEQAFV
                     DLVENPEIVHYCLDKLFDLAYTNTLRIYEQAARSTASILSSYVAEDMGSQESLLFSPA
                     QIHTFLLPRMKRMIDLAHSAGVFVFHHSDGAVRPIIPDMIEAGIDVLNPVQWRCNGME
                     REALKRDFGDRLVFHGGVDNQQTLPFGSVDDVRNEVKENIRILGAGGGYIIAPCHNIQ
                     AVSPAGNVVAMYETGYHEGWT"
     gene            4477..5058
                     /locus_tag="P5179_10155"
     CDS             4477..5058
                     /locus_tag="P5179_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95622.1"
                     /translation="MVDIPVGMYRPHMRDIPLFALPDGFVFRDFRAPGDDGSGDAGTW
                     VRIQAAAEAQFMQITHETFLNDFGGHESQLPGRSWFVTTEKGEDVASITAWWWDTPPD
                     CELQGRVGLIHWVATIPEYQGRKIGKAMMTKAMQRLARDHQVAYLNTSTIRIPAIRMY
                     LDFGFLPDLKKNRADEAWSLVRESLKHSALGNA"
     gene            complement(5067..>5597)
                     /locus_tag="P5179_10160"
     CDS             complement(5067..>5597)
                     /locus_tag="P5179_10160"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRS95623.1"
                     /translation="TISRLTMSSHSGTHLDAPAHVFPGGKTLDEYPPERFFLSAQVVE
                     AKSERVITAADVNALRLEEYEAVLFKTLFIGPPDAHKPLSWISLSPDAAAALVRRGVR
                     LVGIDSPSVDAADATELPVHRTLLGSDVLVLENCDLREVPAGGYELVCMPLRVRTSDG
                     APCRCVLIMNGERATP"
BASE COUNT         1126 a         1655 c         1620 g         1196 t
ORIGIN      
        1 ccacaccagc ggccagagaa tggaactcaa agcgattacg gggcggatca ccacgagact
       61 ggtggattca gaatggcttt tccccagttg tttcggcaat atctccccga aaatcaggat
      121 cacggtcgtc atgagggccg tgttcaccca gacggccgtt tcagggtcgg caatgtagta
      181 gctgagtacc atcgcggtca cagacgccgc accgatatta acgacgttgt tcccgattac
      241 gatggcggtc aggaggcgat ccgagtgccg gtcccactgg aggagaggct ttgccttacg
      301 gccatagtgg tcaagcgcgc gtctgacccg cactttggtc atggcgacaa gcgcggtttc
      361 tgaagcgctg aaaaaggcgg aaagacacag aaggacgaaa aggaccgcaa ggctcgttgg
      421 aacggacacg gtctttctta ctcctcaggc gccgtcaggg atgaagactc cgtgccctca
      481 gaaaggcgag aagctctgcg ggcttgtctg tttccaacgc atcggctccg cattcaattg
      541 cctgtcccca cccgtctggc gtgtctttcg aaagcacgtt acagaacacc cgtgcgcctg
      601 cgcggtgaca ggcggcaacc ttcgcaggcg taatatctcg cagagacgcg cagaagatcg
      661 ccggctgcac tcgttccatg atctccggca gcgtgctgag gtcctccggt ggtcgcggca
      721 acagacggaa tgccgctggt tccaacgctc gcagctccgc aagcgtacgt ccgtcggagt
      781 acaccgccgt ctgctcgctt actccttgcc gacgcattgc ttcgacgagg tcgtgtgcgt
      841 caatcccgcc cgcatcgaga taaatgcgca cgcgtccccg cattgcggcg agggcttcgt
      901 cgagagtggg gattcgaact cccgcggcca gcgacccgtc gggattcttc acgttgaggg
      961 cccggacctc ggccaacgtg agatccgtga cttttcccgt tccgtctgtc gtgcggttca
     1021 ctttctggtc gtgcaaaatt accagcttcc ggtctgccgt gtaagcgggg tcgatttcca
     1081 cccaatcggc gccaacggcg attgcctcct cgattgccgg aacggtgttt tccggctgat
     1141 tcagatgggc acctctgtga gcgatcacct caataacccg ggggggaacg ttctcgcgcc
     1201 tcaggccttt tgcgcggaga aatgcgagaa gttccgccgg tctgtcagtt tgaaggccgt
     1261 ctacgcccat ttccaacgca cgttgccacc cttcgggagc gtcctccgcc atgatgtcga
     1321 cgatcacctt cgccccacgt gaatgacatg cgcgtaccga ttcggaagaa aggtttgcca
     1381 gagaagagat cgcaacgtga tttgtcggta tgctgtcggc aagtgcgtgc catgcggaga
     1441 catcccccgg gtattcagga agcgggcgga tgcgcggctc acgccgaatc atctccctca
     1501 actccgccgg gtcggcatac acaaccgaaa cctccaccaa cccgtgcctt gcgaggagat
     1561 ctgcaacgct atctggaggc gcgtctttgc agtcgacgta tgcgccgacg tgcccacgca
     1621 tgcgcgcgaa tacttcatcc accgtgggaa ttgatgctcc ggcgaatgcc gaggagaact
     1681 tgctgcctgc atcgagcgcc ttgagttccg tcagcgtgag gtctcgcacg cggccctgtc
     1741 cgttcgttcg agcatccacc gtgctgttgt gcaggcatac gaagtgaccg tccccggtta
     1801 cgcggatatc cacttcgatg aggtcaacac cgagtgcgat cgcctgttcg atcgattcga
     1861 cggtgttctc gggacaggat tggtgcgccc ccctgtgggc aaccacgagg atatgtcttc
     1921 gcgattcagt tggtggttcc acgttcgtcc ttagaggtgt gcacccgctc gtcagtaaca
     1981 ggaggcagac cccggttgcg atgcccgaga ccatcatcgc gaagaacccg ccgcgcgcag
     2041 aaatggacga cgtcatctgg tgactcgcac ggcgagcatg cgctcgagcg ccttccttgc
     2101 gttggaggct accgcatcat caacgaaaac ctcgtacctg ccggtacgaa gggaatacca
     2161 aagcttctca ggcgtgttct tctgcatgtg cgggcagagg ttgcacgcac aactgggccc
     2221 gggtgcggct acataggtgt tttccggcgc ttctgttttg aaccggtgca ccatgttgcc
     2281 ctcggtggca acgatgtaag tcttgcctct gttggcagtc acgatgcgca acatcccggc
     2341 agttccgcgg acgaaatctg cttcgtcgcg gatgtttttg gggcactccg gatgcgcgag
     2401 aatctgcgcg tcagggtgtt ccttcttcag ggtacgcagg gtgtctacgc tgaaaagctc
     2461 atgcacgatg caagtggcat tccacaggta aatctccctg tccgtgtttt ccttcaccca
     2521 gccaccaagg tgcacgtccg gcacgaaaag gatcggcgtt ccgcgtggga ccgagttcac
     2581 cacttccagc gcattgccgc ttgtgcacac cacatcgctc agtgctttca ctgccgccgt
     2641 gcaattgaca taacagatgg taaccagatc gtcccgcccc agtttttcct tgagatgcgt
     2701 ctggtaacga gcgagatcct gtgcgtcgca accctccgcc agagagcacc ccgcttcgag
     2761 gtcgggcatg aatacgtggg attcaggatt caggattttc gcagtctccg ccatgaaatg
     2821 gacgccgcaa aagcagatgc gggaagcctt gacctccacc gcctttcggg caagctccag
     2881 cgagtcgccg acgaagtggg cagcgtcctg aatgtgtccg tcctggtaac tgtgtgccag
     2941 gatcagtatt ccgttctggt cagcgaactt gcgcatctca cgtgggtctg gcgccggttc
     3001 caacatcacg ccgtcttccc gcactatggt gtcaacagcc atcaattcgt cctcatatcg
     3061 cttcgctcag atttcttgcc ccttcgcgtt cggcaagagc cttgtgtggt aaatataaag
     3121 gcgtatggaa cacgaaacgg ttccctgagc tgccgcgtcg aagggggctt tgagaacaaa
     3181 gaggtaggaa accgcccgaa attcgcggaa tgatcccgcc gccgcgaaca ctcgtcaacc
     3241 cggcgggaac tcaggtccaa ccttcgtggt accccgtttc atacatggca accacattcc
     3301 cggccgggct cactgcctga atgttgtgac acggcgcgat gatgtacccg ccgccagcac
     3361 caaggattct gatgttttcc tttacctcat tgcgcacatc gtccaccgaa ccgaacggaa
     3421 gtgtctgctg gttatccaca ccaccgtgaa aaacaagtct gtccccgaag tcccgtttga
     3481 gggcttctct ctccatcccg ttgcaacgcc attgcaccgg gttgagaacg tcgattccag
     3541 cctcgatcat gtccgggatt atcggacgaa ccgcgccgtc ggaatgatgg aagacaaaca
     3601 cccctgcgct gtgagccagg tcgatcatcc gtttcatgcg gggcaggaga aacgtgtgga
     3661 tctgcgccgg ggagaaaagg aggctttcct gggaccccat atcttcggca acgtacgacg
     3721 aaagaatgga tgccgtcgat ctggcggcct gctcgtagat ccgcaatgtg ttcgtgtacg
     3781 cgaggtcaaa caacttgtcg agacagtagt gcacgatttc agggttctcg acgagatcaa
     3841 caaaggcctg ttccagcccg cggagctgac agtatcgaag aaacggttcg gagccgccac
     3901 cggcaatcgg acgatgttca tttcccctga tctggtcggg aatgtgagag taatcccacc
     3961 agtctgcctg cggccagcgg taattggctt cgatctcctc gacggaccgg taccgcgcca
     4021 gagggttgtg aacagcctcc gtatacacac cggtggcata ttcgacggtt cggtacctga
     4081 taccgaagac gtcttcgcct tccggcgccg gagggccgac atagttgcca ccaacgctca
     4141 gtgtggcgtc aatgtgaagc ttcgcgtgca gttcctcggt gctgttcacg ccgaggtgcg
     4201 cctgaagctt ccgcgtggcc tccggggtag cccagtagtc catggggatt ctgtcgggtt
     4261 tctggcggcg gagcactgcc tcccagcgtt cgcgcggggt cattgtttct cgtggcatcg
     4321 gcgtccgact ccttttgatg gggtttctcg atgcaagcaa aagatcattg ctcaacaggg
     4381 gggaaccaaa gcagccctct tctctcgtgt gccgggattc ccgtaccttt cggcagcacc
     4441 agttctatga ctcaatctgc gacttgggag gtaaatatgg tggacatacc tgttggcatg
     4501 taccggcctc atatgcgtga tattccttta ttcgcgttgc ccgatggttt cgtttttcgc
     4561 gatttccgcg cgccgggaga cgacggttcc ggtgacgccg gcacgtgggt aagaattcag
     4621 gcggcggccg aggctcagtt catgcagata acacacgaaa cgttcctgaa cgacttcgga
     4681 gggcatgagt cacagcttcc cggtcgaagc tggtttgtca caaccgagaa aggtgaggat
     4741 gttgcgtcca tcactgcatg gtggtgggat acacctcccg actgcgagct tcaaggcaga
     4801 gtcggtctga tccactgggt tgccacaatt cccgaatatc agggcaggaa aatcggaaag
     4861 gcgatgatga cgaaggcgat gcaacgcctt gcccgggacc atcaggtggc gtatctgaac
     4921 acttccacga tccgaatccc tgcgatccgg atgtacctgg atttcggatt tcttcccgat
     4981 ttgaaaaaga atcgtgcgga cgaagcgtgg tcccttgttc gcgagtctct gaagcactcc
     5041 gcgctcggga atgcgtaact gccaccttac ggcgttgcac gctccccgtt cattatcaat
     5101 acgcaacgac agggtgcccc gtcggatgtg cgcacacgca aaggcatgca cacgagttca
     5161 tagccgccag ccgggacctc ccgcaggtcg cagttttcga ggacgagcac gtctgaacca
     5221 agaagcgttc ggtgaacggg gagttccgtc gcgtcagccg catcaacgga cggggaatcg
     5281 atacccacga gtcggacccc gcgccggacg agcgccgctg ccgcgtcagg tgacagggaa
     5341 atccacgata agggcttgtg cgcgtcgggc ggcccgataa agagcgtttt gaacagaact
     5401 gcctcatatt cctccagacg cagagcattc acatccgccg cggtgatgac tcgctccgat
     5461 ttcgcttcca caacctgcgc cgaaagaaag aacctttccg ggggatattc gtccaaggtc
     5521 ttcccgcccg gaaatacatg cgcgggagcg tcgaggtggg tgccgctgtg gcttgacatc
     5581 gtcaagcggc tgattgt
//
LOCUS       DAOTNG010000211         5566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12296_length_5566_cov_5.638904, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000211 DAOTNG010000000
VERSION     DAOTNG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12296_length_5566_cov_5.638904"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..664
                     /locus_tag="P5179_10165"
     CDS             104..664
                     /locus_tag="P5179_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRS95624.1"
                     /translation="MKRKQAVQSIPKEPETRTDSCEPTEEELLRSLDAVIDGYSNTEG
                     ALIPVLQSAQNLFGYLPETVLRHISTRLKLPYSQVTGVVTFYSFFSTVPRGEHVVRVC
                     LGTACYVRGGKEVLATLRKQLGINVGETTEDRKFSLAVGRCFGACSLAPAVMVDETVH
                     QRVRPAKVKDLLAMYREAKPGLREAS"
     gene            694..3813
                     /gene="nuoF"
                     /locus_tag="P5179_10170"
     CDS             694..3813
                     /gene="nuoF"
                     /locus_tag="P5179_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRS95625.1"
                     /translation="MFQDLRKRVVAEQKKRARKRQVYLCTGAGCIASGALDVKKKLDE
                     VMKREFVDFEIRETGCLGPCAKGPALLIQPELTFYENVHPEDAEEIVHQHLKQGKIVE
                     HLLPKNTRQEPVRTEEEMDFFRNQVKIVLRNCGRIDPLKIEDYIGVDGYGALVKVLTS
                     VKPDDVIESIKKSGLRGRGGAGFPTWIKWAATRKSVSDVKYVICNGDEGDPGAFMDRS
                     VLEGDPHSIIEGMAIAAFAVGAKQGYVYVRAEYPLAVARLNHAIDQAREYGFLGKNIL
                     GTSFEFDLEIRMGSGAFVCGEETALMASIEGKRGEPRPRPPFPAEKGLWGKPSLLNNV
                     ETYANVPAIIFRGADWYASLGTAESKGTKVFALAGAVVNTGLVEVPIGISLGELIYDI
                     GGGIKNGKSFKAAQIGGPSGGCIPKEHLNVPMDYRPLTELGAIMGSGGLIVVDDDTCM
                     VDMARFFLEFVQEESCGKCVPCRVGTKRMLEIVTRICEGKGKEGDIEKLIHLGNQIKE
                     TSLCGLGQTAPNPVLSTIRYFREEYEQHIRDRHCEAGVCPSIVRAPCQSACPANVDVP
                     GFVSLVAEKKYAEALQLHRDRNPFASVCARVCFHTCEEKCQRAKLDSAVSIRAIKRFM
                     VEQEVMIQIPEVRENEENAKRKIAIIGAGPAGLSCAYFLARLGYKPTVFEAELRPGGM
                     LVQTIPAYRLPREVLAREIRMIERIGVDIQTGKRLGTDFTLASLKEEGYEAIFLGVGA
                     PESLPIGIPGEDAEGVVQAADFLRTYNIRGSVPVGRKVIVVGGGNAAIDASRSALRLG
                     AEEITILYRRSQAEMPAYAEEIDEALHEGVKLMPLTHPLEILKDANGKVTGVRCTSMK
                     LGEFDRSGRRRPEEKAEFFNLEADQVIVAIGQALRPKMILKDMDIKLNSRNFIQADPI
                     TGETSVPGIFAGGDAVSGPYSVVGAIGAGERAAVGIDHYLTGAFHAFWRREKENTTHF
                     DPESDPVPYGREPIRSLDVQRRRHNFDEVEMPWDEATAVRQARRCLRCDFGKHVPEKV
                     GVDDAQADD"
     gene            3794..>5566
                     /locus_tag="P5179_10175"
     CDS             3794..>5566
                     /locus_tag="P5179_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRS95626.1"
                     /translation="MPKLTINNRSIECENGQTILQAAQAKGIHIPTLCYLEGKDPLGA
                     CRVCLVEVEGAKTLMPSCSTPVVEGMKIHTNNRKVREARKTVVELLLSEHEGECTLCP
                     RTEDCELRELAGQLGIDVVEYEGEKPPRTVDDSTPALVRDSGKCIKCRRCVTVCQEVQ
                     HVGALFPQYRGYETLIGPAFALQLDDVPCVQCGQCAAVCPVGAITEKSHIDAVWKALE
                     DKDKVVVVQTAPAIRAALGECFDLEPGTLVTGRMVTALRELGFDAVFDTNFTADLTII
                     EEGTELLNRLRRTVIDGEKGLLPMFTSCSPGWIQFAEYFYPEFLPNLSTCKSPQQMFG
                     AVAKTFYATEKGIPPENMVVVSVMPCTAKKFECQRDEMTASGYRDVDYVLTTRELGKM
                     ISAAGIDFVNLPDGEMDLPLGLSSGAADIFANTGGVMEAALRTVYELITGRPLPMENL
                     HILPIEGLEGIKQASITIEKPVKEWAFLDNVTVKVAVAHGLSNAAKVLDGIKDGSLDF
                     HFVEVMTCPGGCIGGGGQPRFTTDETRKARIRAIYKEDEGKPVRKSHENPAIKEIYEK
                     FLKKPLGEKSHHLLHTKYQKRVKAG"
BASE COUNT         1232 a         1561 c         1782 g          991 t
ORIGIN      
        1 ctgacacgca ttcgcgtttt tcccagagca ccacggggtc gcgtacatcg tccagttccg
       61 tcgcactaga tccagggttt ggttcactca ccgggagact tgtatgaaac ggaagcaggc
      121 agtacagagc atcccgaaag aacctgagac ccgcaccgat tcctgcgaac caaccgaaga
      181 ggaattgttg cggagcctcg acgccgtcat cgacggttac tccaacacgg agggggcact
      241 tatcccggtg ctccaatccg cgcagaacct cttcggttac cttcccgaaa ctgtactccg
      301 gcacatcagc acccgcctca aattacccta cagccaggtg accggggtcg tcacgttcta
      361 ctcgtttttc tccaccgttc cacggggtga gcacgtggtc cgtgtgtgtc tcggaaccgc
      421 gtgttacgtg cgcggcggca aagaggtgct ggccacgctc cgcaagcagc tcggaatcaa
      481 cgtgggagag acgacggagg acagaaagtt ctcgctggcg gtgggccgct gtttcggcgc
      541 gtgcagcctt gcaccggccg tgatggtgga cgagacggtg caccagaggg tcagaccggc
      601 caaagtgaaa gatcttctgg cgatgtaccg agaggcaaag cccggattga gggaggcttc
      661 gtgatggcag accccagatt aacccgcgta gaaatgttcc aggatttgag gaaacgggtc
      721 gttgccgagc agaaaaaacg agcgcggaag cggcaggtgt acctctgcac aggcgcggga
      781 tgcatcgcat ctggcgcgct ggacgtgaag aaaaaactcg atgaggtgat gaaacgggag
      841 ttcgtggact tcgaaatccg cgaaaccgga tgcctcgggc cctgtgcgaa gggaccggct
      901 ctcctgattc agcccgaact gactttctat gagaacgtgc atccggaaga cgccgaggaa
      961 attgtccacc agcatctcaa acagggcaaa atcgtcgagc atctccttcc gaaaaacacg
     1021 cggcaagaac cggtgcgcac ggaagaggaa atggacttct tccgcaatca ggtaaaaatc
     1081 gttctgcgca actgcggccg cattgatcca ctcaaaatcg aagactacat cggggtggac
     1141 ggatatggag cgctcgtcaa agtgctgacg tccgtcaagc cggatgacgt catagaatca
     1201 atcaagaaat cggggctgcg cgggcgcggc ggcgcaggct tcccgacctg gatcaagtgg
     1261 gcggcaacgc gcaaatccgt aagcgacgtg aaatacgtca tctgcaacgg agacgagggc
     1321 gaccccggtg cgttcatgga caggagcgtg ctggaaggtg acccgcacag catcatcgag
     1381 gggatggcga ttgccgcgtt tgcggtgggc gcaaaacagg gctacgtgta tgtccgcgcg
     1441 gagtatcccc ttgcggtggc gcggcttaac catgccatag accaggcgcg cgagtacggc
     1501 ttcctcggca agaacatcct cggcacgtca ttcgagttcg atcttgaaat ccgcatgggg
     1561 tccggcgcgt tcgtgtgcgg cgaggaaacg gcgcttatgg cgtccatcga gggaaaacgc
     1621 ggcgaaccgc gtccccgccc gcccttcccg gcagagaagg gtctctgggg caaaccgagc
     1681 ctgctcaaca acgtggagac gtacgccaac gtcccggcga tcattttccg gggtgccgac
     1741 tggtatgcct cgctgggaac ggcggaaagc aaaggcacga aggtgtttgc gctggcaggc
     1801 gcggtggtga ataccggtct ggtggaagtt ccgatcggaa tcagtctcgg cgagctcatt
     1861 tacgacatcg gcgggggtat caagaacggg aaatcgttca aagctgccca gatcggcggc
     1921 ccatcaggag ggtgcattcc gaaagagcac ctcaacgttc cgatggacta ccggccgctt
     1981 accgagcttg gcgccatcat gggctctggc ggccttatcg tggtggatga cgacacctgc
     2041 atggtggaca tggcgcggtt cttcctcgag tttgtgcagg aggaatcttg tggcaaatgc
     2101 gtgccatgcc gcgtcggcac caaacggatg ctggaaatcg tcacccgcat ctgtgagggt
     2161 aagggcaagg aaggcgatat cgagaagctg atccacctcg gcaatcagat caaagagacg
     2221 tcgctgtgcg gcctgggtca gacggctccc aacccggttc tcagcacgat ccggtatttc
     2281 cgggaggaat acgagcagca cattcgcgac cggcattgcg aagcgggcgt ctgtccgtcc
     2341 atcgttcggg ctccgtgcca gagcgcgtgc ccggccaatg tggatgtgcc ggggttcgta
     2401 tcgcttgttg cggagaagaa atacgcggag gcgctgcagc tccatcgcga ccgcaacccg
     2461 ttcgcctcgg tgtgcgcgcg ggtgtgcttc cacacatgcg aggaaaagtg ccagcgcgcc
     2521 aaactcgatt cggccgtatc cattcgcgcg atcaagcgat tcatggtcga acaggaagtg
     2581 atgatccaga ttcccgaagt gcgggaaaac gaggaaaacg ccaagcggaa gatcgccatc
     2641 atcggcgcgg gcccggcagg gctctcctgt gcgtatttcc ttgcgcggct cgggtacaaa
     2701 ccgaccgtgt ttgaagcgga gcttcgtccg ggtggaatgc tggtccagac aatccctgcg
     2761 taccggctgc cgagggaagt tctggcccgc gaaatccgca tgatcgagcg gatcggggtc
     2821 gacattcaga ccggaaagcg tctcggtacg gatttcaccc tcgcgagcct caaggaagag
     2881 gggtacgagg cgatcttcct cggcgtaggc gcaccggaat ccctgcccat cggcattccg
     2941 ggagaggatg cggagggcgt ggttcaggcg gcggatttcc tccgcacata caacatccgc
     3001 ggatcggtgc cggtcggcag aaaggtgatc gtggtgggtg gcggcaatgc ggcgattgat
     3061 gcctcccgca gtgcgctgcg tctcggcgcc gaggaaatca cgatcctcta ccgccggagc
     3121 caggccgaaa tgccggcgta cgcggaagaa atcgacgagg cacttcacga aggcgtgaaa
     3181 ttgatgccgc tcacccatcc gctggagatc ctcaaggatg ccaacgggaa ggttacggga
     3241 gtgcggtgca cctccatgaa actcggcgag ttcgaccgga gcggaagacg gcgtccggag
     3301 gagaaagccg aattcttcaa ccttgaagca gatcaggtca tcgtcgccat cgggcaggcg
     3361 ctgcgtccca agatgattct gaaggacatg gatatcaaac tcaactcgcg gaacttcatc
     3421 caggcggatc cgattaccgg cgaaacttcc gtgccgggga tattcgcggg cggagatgcc
     3481 gtgagcgggc cgtattccgt cgtcggggcg attggcgccg gcgaacgggc ggccgttggc
     3541 attgatcact acctcaccgg agcgttccat gcgttctggc gcagggaaaa ggagaacacc
     3601 acgcatttcg atccggaatc ggatcccgtg ccgtacgggc gagaaccgat tcgctcgctg
     3661 gatgtgcagc gcagaagaca caatttcgac gaagtagaaa tgccatggga cgaagccact
     3721 gccgtgcgtc aggcaaggcg ctgtttgcgg tgcgatttcg gcaaacacgt tccggaaaaa
     3781 gtgggggtgg acgatgccca agctgacgat taacaatcga agcatcgaat gtgagaacgg
     3841 ccagacgatc cttcaggctg ctcaggccaa ggggatacac ataccgacgc tctgctacct
     3901 cgaaggaaag gaccccctcg gcgcatgccg ggtctgtctc gtggaggtag aaggcgccaa
     3961 aacgctcatg ccgtcgtgct ccactccggt ggtcgagggt atgaagattc atacgaacaa
     4021 ccggaaagtg cgcgaggcaa gaaagacggt cgtcgagctt ctccttagcg aacacgaagg
     4081 ggaatgcacg ctctgcccga gaaccgagga ctgcgaactg cgtgaacttg cgggccagct
     4141 cgggattgac gtggtggagt atgagggcga gaagccgccg cgcacggtgg atgactccac
     4201 gccggcgctc gtgcgcgatt ccggcaagtg catcaagtgc cggagatgcg tcacggtctg
     4261 tcaggaagtt caacacgtcg gcgcgttgtt cccgcagtac cgggggtatg agacgcttat
     4321 cgggcccgca ttcgcactcc agcttgacga cgtaccgtgc gtccagtgcg gccagtgcgc
     4381 ggcggtgtgc cctgtgggcg cgatcaccga aaaaagccac attgatgcgg tctggaaggc
     4441 gcttgaggac aaggacaagg tcgtcgttgt tcaaaccgcg ccggcgatcc gggcggcgct
     4501 cggggagtgt tttgatctcg aaccgggaac gctggtaacc gggcgcatgg tcactgccct
     4561 gcgcgaactc gggttcgatg cggtattcga cacgaacttc accgccgacc tcacgatcat
     4621 cgaagagggc acggagcttc tgaaccgcct tcgcagaacc gtgatagacg gggaaaaagg
     4681 cctcctcccg atgttcacga gctgttcgcc ggggtggatc cagttcgcgg agtacttcta
     4741 tcccgaattc cttcccaacc tctccacctg caaatcgccg cagcagatgt tcggtgccgt
     4801 ggcaaagacc ttttatgcca cggaaaaggg tattccgcca gagaacatgg tcgtggtctc
     4861 cgtcatgccg tgcaccgcga agaaattcga gtgccagcgc gacgaaatga ccgcgagcgg
     4921 gtaccgggat gtggactacg tcctgaccac gcgtgagctg ggcaaaatga tcagcgcggc
     4981 gggtattgat ttcgtgaacc tgcccgacgg tgaaatggac ctgccgctgg ggctttcttc
     5041 aggtgcggcg gacattttcg cgaacaccgg cggcgtgatg gaagcggcgc tgcgcaccgt
     5101 ctacgaactc atcaccggcc ggccgctgcc catggagaac ctccatatcc tccccatcga
     5161 ggggctggag gggatcaaac aggccagcat caccatcgag aaaccggtca aagagtgggc
     5221 gttccttgac aacgtaacgg tgaaagtcgc cgtggcgcac ggtctttcga acgcggccaa
     5281 ggtgctggac ggcataaagg acggctcgct ggacttccat ttcgtggaag tgatgacgtg
     5341 ccccggcgga tgcatcggtg gtggcgggca accgcgcttc accacggacg aaacccggaa
     5401 agcgcgcatt agagcgatct acaaagagga cgaaggaaag ccggtgcgca aatcgcacga
     5461 aaacccggcg atcaaggaga tttacgagaa attcctcaag aagcctctgg gcgagaaatc
     5521 gcatcatctg ctgcacacga agtaccagaa acgcgtgaag gcgggt
//
LOCUS       DAOTNG010000212         5565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12302_length_5565_cov_5.233394, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000212 DAOTNG010000000
VERSION     DAOTNG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12302_length_5565_cov_5.233394"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="P5179_10180"
     CDS             <1..745
                     /locus_tag="P5179_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRS95627.1"
                     /translation="RPTTIPQLLDELKLEQAIAREALRLAFIQHKQLAVGLKGIAKER
                     TLSDVFSQESDGSLINMMELDLLVGSGGVLSHAPRRQQSMQMMIDAFQPEGVTRLAVD
                     SIFMMPHLGVLSTVNETAATEVFEKDCMIYLGTCVAPRGKGKKGNQVLSFEMEFQDGR
                     RESGTMNVGDLRHFPLGLNETAKLRATPAKGWDLGHGSGKPVEAVIHGGVVGIVLDTR
                     GRPIQFAADRPAQIADTVRWNKATDQFPV"
     gene            763..1884
                     /locus_tag="P5179_10185"
     CDS             763..1884
                     /locus_tag="P5179_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95628.1"
                     /translation="MAHSYTPGLRVTPFATLQRRRILPLKGEVTVRVGDLVSRDTVVA
                     RTELPGDVATVNVVNRLGIEAHQIEQYMVKKVGDSVATDELIAETRPFFKFLKSTVNS
                     PIDGTVESVSTVTGQVILRRPPKPVAVTAYVDGRVIEEIPNEGVVVETQGAFIQGIFG
                     IGGEAWGKIRRVVESHDSVATPSSISADMEGCVLICGSLVSADTIKRAIAVKAAGVIA
                     GGIHAGDLKDLLGYDLGVAITGTERIGTTIIVTEGFGAIAMARRTFDILSSCEGMVAS
                     INGTTQIRAGVQRPEIIVSRPPSPSDTEVAASSAGLQIGDPVRVIREPYFGRLGSVSA
                     LPSELQTVESETRVRVLEVALEDGEKVIVPRANVESIAL"
     gene            1960..3258
                     /locus_tag="P5179_10190"
     CDS             1960..3258
                     /locus_tag="P5179_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95629.1"
                     /translation="MQRLTQFACIAALFWTGTALASDAVETGTGSKTPVPDSVRALPP
                     PLPTQIRAADVPSDNGKAVVVTWLPDASTPSLVRYQVGVATNPSGPYYFGGEAPAAGP
                     LVKDDPKIFGWSPANPDHQFLVVSSYMVPPATPEGNPERVELRPDQTYYLRFAVVGDS
                     ASAELAAPMSVTPRQNWIKLHKLNNLIFGLFFGVVILSFIEFARRNPNMFIRKIAGLD
                     AVEEALGRATEMGKPVYFVHGLNSMGEVSAIASVNILGRVARRVAQFDTTLKVMNNDP
                     IVLAVSQEVVREACIDVGRPDAYVPDNVMFVADRQFSYAAAVDGMMMRERPATVMLMG
                     YFYAEALLFAETGNVVGAIQIAATDSYTQIPFFITTCDYTLIGEELYAASAYLAREPK
                     LLGSLRGQDVGKAVLIAILTIGTALELAGIHWLTHLVAPL"
     gene            3292..3945
                     /locus_tag="P5179_10195"
     CDS             3292..3945
                     /locus_tag="P5179_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95630.1"
                     /translation="MIFFKRTLPLVIAFVMGIVAISIYYIPHQAAVDLNDLLSKWDRI
                     IAAFAAFLGVYSLLYLHAGRLKRRQAGWGYSVFVYLGFVVMLTAAIYNNGAGPLVALR
                     KGGAYDWLYTNVFVAADASMFAILSFFIASAAYRTFRARTTEATILLVAAVIVMLGRV
                     PIGALLWESMPELQQWLMNVPNMAAKRGILLGVYLGAVATALRIIFGIERSYLGGDE"
     gene            3942..4802
                     /locus_tag="P5179_10200"
     CDS             3942..4802
                     /locus_tag="P5179_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95631.1"
                     /translation="MIQKLLNIDRRWIFLFIFVGVAAAMLFPLPLPIRPSSDVKNVYN
                     TIESIKGKPNATVLLSFDYGPGSEPELQPTAISIIRQCFRNNIKVVGMALWPDAVGLA
                     QAAFDSVSRAEGKEYGKDWTFLGYKTGSVTLILNMGEDFHGAFNVDYRGNKTSEMPVT
                     QHIAKLGSFDYVVTLAAGKTIDGVWVTYAVDRYRIKLGGATTAVTSPDMFPYLQSGQL
                     NGLVSGLAGAAEYETLVKTQGSATRGMQPQSAAHLIIIISIIIGNTMYFVQRRRDRRQ
                     SVSGNEGTRS"
     gene            4799..>5565
                     /locus_tag="P5179_10205"
     CDS             4799..>5565
                     /locus_tag="P5179_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95632.1"
                     /translation="MMHNPGSRTATFVFAGIFLALSIWQLAAGHIALTADGLGVLTAA
                     GLTLALFSFLYDDNPLFKIAEHLYVGVAAAYGLALVWFQVILADLVNPIFFSTATIRQ
                     LWVLIVPSVLGILLIMRIVPKFSWLSRISFSYVVGFGAGISIPAYIASQLLEQVHPTL
                     APALGWDGGDKFILMAAIVLIAVAYLVSRRGGGDLGMNSWFGPTLLSVVFLICYFQGV
                     GAASLVLVGVIAVLFYFFFSVEHTGAVGAISRVGIWFL"
BASE COUNT         1143 a         1554 c         1659 g         1209 t
ORIGIN      
        1 acgtccaacg accatccctc agctccttga tgaactgaag ctggaacagg caattgcgcg
       61 cgaagccctg cgactggcat tcatacagca caagcagctt gccgtgggtt tgaaaggcat
      121 cgccaaagaa cggactcttt ctgacgtgtt ctcgcaggag agtgacggca gcctgatcaa
      181 catgatggag ctggacctgc tggttggctc cggtggcgtc ctcagccatg ccccgaggcg
      241 gcagcaatcc atgcagatga tgatcgacgc gttccagccg gaaggcgtca cacgccttgc
      301 cgtggacagc atcttcatga tgccccatct gggcgtgctg tcgaccgtca acgaaactgc
      361 cgccactgag gtgtttgaaa aggactgcat gatatacctc ggcacctgcg tggcgccgcg
      421 aggaaagggg aaaaagggta accaggttct ttcattcgaa atggagttcc aggacggacg
      481 ccgggaaagc ggcacaatga atgtcggcga cttaagacat ttcccactcg gtttgaacga
      541 aacagcgaag ctccgggcaa cacccgccaa aggctgggac ctcggacacg gatcaggcaa
      601 gccagtggaa gccgtaatcc acgggggagt cgtcggaatc gtgctcgaca cgcgcggcag
      661 acccatccag ttcgccgctg atcgcccggc gcagatcgct gacaccgtcc gttggaacaa
      721 agcaaccgat cagtttcccg tctaacccct gtaagggtat ctatggcaca ctcctacact
      781 cctggcttac gcgtaacacc gttcgcgacg cttcaacgtc gacgtatact ccccctgaag
      841 ggggaagtca cggttcgagt tggcgacctc gtttccaggg ataccgtggt cgcgagaact
      901 gagcttccgg gcgatgtggc tacggtgaac gtggtgaacc gcctcgggat agaagcccac
      961 cagattgagc aatacatggt gaagaaggtg ggcgattcag tcgcgacgga cgaacttatc
     1021 gcggagacgc gcccgttttt caagtttctg aagtccacgg tgaactcgcc catagacggc
     1081 acagttgaga gtgtttccac ggtcaccggc caggttattc tgcgccggcc accaaaaccg
     1141 gtcgccgtaa ctgcgtacgt ggatggcagg gtaattgagg aaatcccgaa tgaaggcgta
     1201 gtggtcgaaa cgcagggcgc cttcatccag gggattttcg ggatcggcgg cgaagcctgg
     1261 ggcaagattc ggcgtgtggt ggaatcccac gattctgttg cgacaccttc ctcaatttcc
     1321 gccgatatgg aaggctgcgt gcttatctgt ggatcactcg tctccgcaga cacgatcaag
     1381 cgggcaatcg cggtgaaggc cgccggagtg atcgcgggcg ggatccacgc aggcgacctg
     1441 aaggatctgc ttggatatga tctgggggtg gcaatcacag gaacggaacg gattggaacc
     1501 acgatcatag tcaccgaagg cttcggggcg attgcaatgg cgcggcgaac tttcgatatc
     1561 ttgagttcat gtgaaggcat ggtcgcctcc atcaacggca ccacacagat ccgtgccggc
     1621 gttcagcggc ctgagattat cgtttcgcgt ccgccgtcac catctgacac ggaggttgca
     1681 gcaagcagcg cgggtctcca aatcggcgac ccggtgcgcg tgattcgcga accgtatttc
     1741 gggcggctgg gttcggtaag cgctctcccc tccgagctcc agacggtgga atccgaaacc
     1801 cgtgttcgcg tgcttgaggt ggccctcgag gacggagaga aagtcatcgt gccgagagcc
     1861 aacgtggaat cgatcgcact ctagtgcatt gttctggggt gagtccagtg ataacacccg
     1921 cgccgtccgc gtcgtccgga ggtgccagga ggacaaccag tgcagagatt gacgcagttc
     1981 gcgtgtattg cagcgctttt ctggaccggg acagcgctgg cgtctgacgc ggtagagacc
     2041 gggaccggca gcaaaacgcc ggttcctgat tcagtccgtg cactaccccc gccccttcct
     2101 acccagatcc gagccgcaga cgtacccagc gacaacggga aggcggtggt ggtaacctgg
     2161 ttgccggacg cttcgactcc aagcctggtt cggtaccagg ttggcgtggc tacgaacccg
     2221 tcaggaccgt actacttcgg cggcgaagca cctgccgcgg ggcctcttgt gaaggatgac
     2281 ccaaaaatct tcggttggtc tccggcgaat cctgaccacc agtttctcgt cgtttcatcc
     2341 tacatggtgc cgccggcaac gccagagggt aacccggagc gcgtggaact acggccggat
     2401 cagacgtact acctgcgctt cgcggtggtc ggcgattccg cgagcgccga gttggccgct
     2461 ccgatgtccg tgacaccgag gcagaactgg atcaagctcc acaaattgaa caacctgata
     2521 ttcggcctct ttttcggcgt ggtcatcctc tctttcatcg aattcgcgcg gcgcaatccg
     2581 aacatgttta tacggaaaat cgccgggctt gacgcggtgg aagaggcgct gggacgcgcc
     2641 accgaaatgg ggaaacccgt gtatttcgtt cacgggctga actccatggg agaagtgtcc
     2701 gcaatcgctt ccgtgaatat tctcggccgc gtggcacgaa gagtagcgca attcgatacg
     2761 acactgaagg ttatgaacaa cgacccgatc gtgctggcgg tgagccagga agtggtgagg
     2821 gaagcgtgta ttgacgtagg gcgcccggat gcctacgtcc cggacaacgt catgttcgtt
     2881 gcggatcgcc agttcagcta cgcagccgca gtggacggaa tgatgatgcg agagcgcccg
     2941 gccaccgtga tgctgatggg atacttttac gcggaggcgc ttctcttcgc ggaaacgggg
     3001 aacgttgttg gcgcgatcca aattgccgcc accgattcgt atacgcagat tccctttttc
     3061 atcacgacat gcgactatac gctgatcggt gaagagctgt acgccgccag cgcgtatctc
     3121 gcgcgggaac ccaaactcct cggaagcctt cgaggacagg atgtcggaaa ggctgtcctg
     3181 attgccattc tgacgatcgg aaccgcactg gaactggcgg gcatccactg gttaacacat
     3241 ctggtagctc ctctctgaat ttgcggacgc gagtatggag gctgagaagg aatgattttc
     3301 ttcaaacgca cgttgccact tgtcatcgcg ttcgtgatgg gtatcgtggc catatcgatt
     3361 tactacatcc cccatcaggc cgcggtagac ctgaacgacc ttttgtccaa gtgggaccgg
     3421 atcattgcgg cttttgccgc attccttggc gtgtacagcc tgttgtacct tcacgcagga
     3481 cgcttgaaac gcaggcaggc cggctgggga tacagcgttt ttgtgtacct cggtttcgtg
     3541 gtcatgctga cggccgcgat ttacaacaat ggcgcgggcc ccttggttgc gttgcgcaaa
     3601 ggtggggcgt acgactggtt atacacgaac gttttcgtgg ccgccgatgc gtccatgttc
     3661 gcaattcttt ctttcttcat cgcatctgca gcctaccgaa ccttccgagc ccgaacgact
     3721 gaggcgacga tcctgcttgt ggcggccgtc atcgtcatgc tgggacgcgt gccgataggc
     3781 gcgcttctgt gggaatcgat gccggaactg cagcagtggt tgatgaacgt tccgaatatg
     3841 gcggcgaagc gcgggatcct gctgggcgtt tacctgggtg cagtggcaac cgccctgcgc
     3901 atcatcttcg gtatcgaacg ttcctatctc ggaggtgatg aatgatccag aaactcctga
     3961 acatcgaccg tcgctggatc ttcctcttta tcttcgtcgg ggtagccgca gcgatgcttt
     4021 ttccgttgcc gctgccaatc cggccttcct ccgacgtgaa gaacgtgtat aacacgatcg
     4081 agtccatcaa aggaaaaccg aacgcaacgg tgcttctttc attcgattac ggcccaggaa
     4141 gtgaacccga acttcagccc actgcgataa gcatcatccg ccagtgtttt cgaaacaaca
     4201 tcaaggtcgt cgggatggct ctctggcccg acgcggtggg attggcccag gcggcgttcg
     4261 attccgtgtc gcgggcagaa ggaaaagaat acgggaagga ttggaccttt ctcggataca
     4321 agaccggttc cgtgacgctg atcctgaaca tgggagaaga cttccacggg gccttcaatg
     4381 tggattaccg cggaaacaaa acgtcggaaa tgcctgtcac ccagcacatt gccaagctgg
     4441 ggagctttga ttacgtggtc actctcgcag cgggaaaaac catagacggc gtctgggtca
     4501 cctacgccgt tgaccgctac cgcatcaaac tcggcggcgc gacaacggca gtcacgtcgc
     4561 ctgatatgtt cccgtacctc cagtccggcc agttgaacgg gctggtatca gggttggccg
     4621 gtgcggcgga atacgagacg ctggtgaaaa cgcaggggtc ggcaacacgg ggtatgcaac
     4681 ctcaatctgc cgcgcatctt atcatcatca tctccatcat tatcggcaac acgatgtatt
     4741 tcgttcaacg gagacgagac cgcagacaga gtgtttccgg caacgagggg acacgatcat
     4801 gatgcacaat ccaggaagcc ggaccgcaac gtttgtgttt gcaggcattt tcctcgcact
     4861 gagcatctgg caactcgccg cgggacatat cgcgttgaca gcggacgggc tgggagttct
     4921 cactgccgcc ggactcactc tcgcgctgtt cagtttcctt tacgatgaca acccgctttt
     4981 caagattgcc gagcatctgt acgtgggtgt ggcggcagca tacggacttg cgctggtgtg
     5041 gtttcaggtg atcctcgcag acctcgtgaa tccgattttc ttctcgacgg ccacgattcg
     5101 ccagttgtgg gttctcatcg ttccatcggt tctcggcatt cttctgatca tgcgaatcgt
     5161 acccaaattc agctggttga gcaggatatc gttttcctac gtggttggat tcggggccgg
     5221 tataagtatt cccgcgtata tcgcctccca gcttctggaa caggtacatc caacgttggc
     5281 gcctgcgctc gggtgggacg gaggagacaa gttcatcctt atggcggcga tagttcttat
     5341 cgccgtcgca tatctggtgt cgcggcgtgg agggggcgat cttggaatga acagctggtt
     5401 cggtccgacg ctcctttcgg tcgtcttcct catctgttac ttccaagggg tgggggcggc
     5461 ctcacttgtt ctggtcggcg taatcgcggt cctgttttat ttcttctttt ccgtggagca
     5521 caccggtgcg gtcggagcca tttcgcgtgt cggaatctgg ttcct
//
LOCUS       DAOTNG010000213         5442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12631_length_5442_cov_4.788008, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000213 DAOTNG010000000
VERSION     DAOTNG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_5442_cov_4.788008"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..2795)
                     /locus_tag="P5179_10210"
     CDS             complement(108..2795)
                     /locus_tag="P5179_10210"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRS95633.1"
                     /translation="MQGNTELAARYDHREVESRWYDFWEQKNAFHAQDVSEKEPFCIV
                     IPPPNVTGSLHMGHALTVTIEDIMIRWHRMLGHNTLWMPGTDHAGIATQMVVERELKR
                     SEHKTRHDIGREEFVKRVWQWKEQYGNRISLQLRALGSSLDWQRERFTMDAQLSRAVR
                     EVFVRLYEEGLVYRAHRLINWCPRCQTALSDLEVEHDEGHAGSLWHIAYPVTGEPGRE
                     VVVATTRPETMLADTAVAVHPEDERYTDLVGKTVTLPLVGRQIPVVADDILVDREFGS
                     GVVKVTPGHDFNDFETGLRHNLPMISMLDSWAKTTDVCGKYAGVDRFEARKMVLADLE
                     VEGFLRKTESHSLSIGKCQRCETIVEPMLSDQWFVRTKPLAEPAVAAVEDGRVRIIPE
                     SHTKTYFHWMRNIQDWCISRQLWWGHQIPAWYCRTCGHVNVSREDVTACAACGSGEVV
                     RDEDVLDTWFSSALWPFSTLGWPEDTPALKTFYPTNVMETGYDILFFWVARMMMMGMK
                     FMGEPPFKTVYLHGMVLDKHGQKMSKVKGNVVDPLDVTQEVGADSLRFYLAVMSGQER
                     SLRFDMERVVGYRNFINKVWNAARFALNHISELPAEPFSPQTLVLSLADRWILSRLAQ
                     TVSRVSSALEEYRFDIAADTLYHFFWHEFCDWYIEMVKTPLADDTHTERKHAAMRVLA
                     HALDVSLRLLHPIMPYVTEELWQKLPVPRHSASMSGVTDTIVLASYPRPDAESRDEQA
                     EREMAAIQETVTAIRTIRSETRVPPAAKIAVVVRADGFVRATLERHASYVTDLARLES
                     LTVSSDASRPRASATAVVQGAAIYVPLEGLIDFEQEKQQLAKEIARIESYVAATRKKL
                     GNESFVSRAPAEVVQTEREKIAAAEASLERLRDTLVSIG"
     gene            complement(3008..3667)
                     /locus_tag="P5179_10215"
     CDS             complement(3008..3667)
                     /locus_tag="P5179_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95634.1"
                     /translation="MSKAPNPEDTGQNPANSRGRSLRLILGAGLFFFLGVVVGWAFHA
                     ALVTDGRLSSGSPGTSSLDQQIVVLRKRIAESERDLELGDDAAPTRILLGNTYFDLGS
                     ALAVSGDSSAAMAAFERAIVHYEKARSAGAASADVLTDLGTMYFRVRRPREAIEAYER
                     AIAADPAHLNAWMNLGVVRKEAFGDTAGARQAWERVLAIDGSGPEAARVRSWLGSTRG
                     E"
     gene            complement(4082..4960)
                     /locus_tag="P5179_10220"
     CDS             complement(4082..4960)
                     /locus_tag="P5179_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95635.1"
                     /translation="MEDAHMVRIGIVGSDNSHAEAFSKLCNVPQDLTVADAKVVAIFG
                     VDPARTKEVATLGNVPRIVDRPEEMIGSVDAVLVVFRHGGLHRQYAEPFLRAGIPVFV
                     DKPLAISPDDARALLDLAEAKNTPLTSFSTLRYSRDMLAFIKEAEEVGPVSLSVFSGP
                     ADRQSEYGGLPFYGIHTVELAQALNGPGVISVHAVENKKNIVATLMYEDGRVTVLNFL
                     GDAAYVFHMLAFGKKGHVGRALDAGTCYKDGLEIVLAMVRTGKRPLERNELLEPVLIL
                     DAIERSLGTGKAVRVV"
     gene            complement(5029..>5442)
                     /locus_tag="P5179_10225"
     CDS             complement(5029..>5442)
                     /locus_tag="P5179_10225"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS95636.1"
                     /translation="HLKGIKDSSGDLERMKNELEMGLPDFQVISGADHRTLDALRLGA
                     KASVTSTANAFPEVFQAVYDAFNAGDMRRAEEAQERLTALTKILMSGRYLATYKTALR
                     LRGIDIGTVRPPHRELTDTEAHKLTVGLKDMGLIP"
BASE COUNT         1101 a         1654 c         1507 g         1180 t
ORIGIN      
        1 tccgcgtaac ggggtgcggt ttcattcggc atttttcgtc gccctcggct gggaattttt
       61 gtcgagttct gttttccgcg gcagggtcac aaactcgcgg gcgcagttca ccctatcgat
      121 acaagtgtat cccgcaatcg ctcgagtgat gcctccgccg ctgcaatttt ctcgcgttcg
      181 gtttgaacga cctccgctgg ggcccggctc acgaagcttt cgtttcccag tttctttcgc
      241 gtcgccgcca cgtacgattc gattcgtgca atttcctttg ccagctgttg tttctcctgc
      301 tcaaagtcga taagaccctc aaggggaaca tatatggctg ctccctgaac aacggccgtg
      361 gcggacgccc tcgggcgtga ggcgtcgctc gaaacggtga gcgactccag ccgcgcaagg
      421 tccgtcacat aggaggcgtg tcgctccagc gtcgcgcgca cgaaaccgtc cgcgcggacg
      481 accactgcta tcttcgcggc cggtggaaca cgtgtttcgc ttctgatcgt ccggatcgca
      541 gttactgttt cctgtatcgc tgccatctcc cgttccgcct gttcgtcccg cgattcggca
      601 tccggcctgg gataggacgc cagcacaatc gtgtctgtta ctccgctcat cgaagcactg
      661 tgccgcggca cgggtagttt ctgccagagt tcctcggtga catacggcat gattggatgg
      721 agcaggcgca ggcttacatc aagcgcgtgc gcgagtactc gcattgctgc atgctttctt
      781 tccgtgtgag tatcgtcagc aagcggcgtc ttgaccatct cgatatacca atcacagaac
      841 tcgtgccaga agaaatggta caacgtgtcg gcagctatgt cgaatcgata ctcctcaagt
      901 gcgctggaaa cgcgcgaaac ggtttgcgcc agtctgctga gaatccatct atccgcaaga
      961 gaaaggacaa gcgtctgcgg ggagaaaggc tcggcaggaa gctcgctgat gtgattcaac
     1021 gcgaaccgcg cggcgttcca gactttgttg atgaaattcc tgtacccaac gactcgttcc
     1081 atgtcaaagc gcaggcttcg ctcctgcccc gacatcaccg caaggtagaa cctcaggctg
     1141 tccgcgccca cttcctgcgt cacatcgagc ggatccacca cgttcccttt gaccttgctc
     1201 atcttctggc cgtgtttgtc cagaaccatc ccgtgcaggt acaccgtttt gaacggtggt
     1261 tcacccatga acttcatccc catcatcatc attctggcta cccagaagaa gaggatgtcg
     1321 tatccggttt ccatgacgtt ggtggggtag aacgttttga gagcgggcgt gtcttccggc
     1381 cagccgaggg tagagaacgg ccacagcgcg gaactgaacc acgtatcgag gacgtcctca
     1441 tcgcgaacca cctctccgct gccgcacgcg gcgcacgcag tcacgtcctc acggctgacg
     1501 ttcacgtgac cgcacgtccg gcagtaccac gcgggaatct gatgccccca ccacagctgg
     1561 cggctgatgc accaatcctg aatgttgcgc atccagtgga aatacgtctt cgtgtgtgat
     1621 tctggaatta tgcgaacacg accgtcttca accgccgcaa cggcaggctc tgcgagcggt
     1681 ttcgtccgga cgaaccactg gtcgctcaac atcggctcga cgatggtttc gcaacgctgg
     1741 cattttccga tggaaagcga atggctctcc gtttttctca ggaagccctc tacttcgagg
     1801 tctgcgagca ccatttttct ggcttcgaac ctgtccactc ctgcgtactt cccgcacacg
     1861 tcggttgttt tcgcccagga gtcgagcatg cttatcatgg gcaggttgtg gcgcaggccc
     1921 gtctcgaaat cgttgaaatc gtgcccgggc gtgaccttta ccacccctga accgaactcc
     1981 ctgtcgacga gaatatcgtc ggcgaccacg ggtatttgtc tgccgacaag aggcaacgtc
     2041 acggttttgc cgacaagatc tgtgtaccgc tcgtcctcag gatgaacggc gactgcagtg
     2101 tccgcgagca tggtttcggg gcgcgtggtg gctaccacca cttcgcgacc gggttcgccc
     2161 gtaacagggt aggctatgtg ccagagactt ccggcatgtc cctcgtcatg ttcgacctcg
     2221 aggtcgctga gcgcggtctg gcatcgcggg caccagttga tcaatcgatg ggcgcggtag
     2281 acaagacctt cctcgtacaa gcggacgaac acctccctca ccgcgcggga gagttgagcg
     2341 tccatggtga agcgctcgcg ctgccagtcc agcgaggaac cgagcgcgcg gagctggagg
     2401 cttatacggt ttccgtactg ctccttccat tgccagacgc gcttgacgaa ttcctcgcgc
     2461 cctatgtcat gccgggtttt gtgttcactt cttttcaatt cccgttccac gaccatctgt
     2521 gttgcgatgc cagcgtgatc agttcccggc atccacagtg tattgtgccc gagcattcga
     2581 tgccaccgga tcatgatgtc ctcaatagtc acggtgagcg cgtgccccat atgaagactg
     2641 cccgtaacat tcggtggcgg aattacaatg cagaaaggtt ccttttcgga gacgtcttgc
     2701 gcgtggaagg cgttcttttg ttcccagaag tcgtaccacc gggattcgac ctccctgtgg
     2761 tcgtaacgag ctgccaattc ggtattaccc tgcatgcaga tgcctttcga tcgggactgt
     2821 atgtccggga gggacagaag gtcaaagcaa aaaggcggga cgcggctcag cgccgcgcgc
     2881 gtacggtaga catgaagctg aactccatgg agtccgtgtt cctcgcaaac cgacaatcaa
     2941 tcaaaatatc gcacaaaagc ccacgcctgt cacctcggtt cggggtaagt tgacctgccg
     3001 accatcctca ttcacctcgg gttgagccga gccatgaacg cacccgtgct gcttcgggac
     3061 cggaaccgtc aatggcaaga accctttccc aggcctgccg cgcgcccgcg gtatcgccga
     3121 atgcctcctt gcggacaacg ccgagattca tccacgcatt gaggtgagcc gggtccgccg
     3181 caatcgcacg ttcgtacgcc tcgatggcct cacggggtct gcgcacccgg aaatacatgg
     3241 taccgagatc cgtcaacacg tcggcggatg cagccccggc ggaacgcgcc ttctcgtagt
     3301 gaacgatggc tcgttcaaac gcggccattg ccgcggagga gtctccggaa accgccagcg
     3361 cggatccaag atcgaaataa gtgtttccga gcaggatgcg cgtgggtgcg gcgtcatccc
     3421 ccagctccag atcccgctcc gattctgcta tccgtttgcg aagaacgact atctgttggt
     3481 ccagcgaaga ggtgcccggg gagccggagg agaggcgacc gtccgttacg agggcggcgt
     3541 gaaaagccca gccaacaacg actccaagga aaaagaaaag gcctgcgccc aaaatcagtc
     3601 ggagtgaacg gcccctggaa ttcgccgggt tctggccggt atcttccggg ttcggagcct
     3661 tagacacggt tgtggagcct ccactgcgct gcatccgcgg cggaacgcct tggtggtccc
     3721 gcgctgaccg cagtctcacc cattcccaaa atgagaatat cgactaaata cagttgggca
     3781 aaatacttac agcgatacta ctaccgtagg tgctggcaac acgtctcagt atgagacact
     3841 tctgcgtggg tgcctgagat gccggcaccg agaaaatgcc ggaattccgg catttgtacg
     3901 tgttggcatc ctgcttgcta actcaatgac cggattcgga acgggccgtg caaagggtcc
     3961 gaaaccggaa aaaaggagcg gcttggggcg ggatgggggg ctatcccacg cgccaagccg
     4021 ccggaagggg gctcgagcgg caacaggcgc cgccgggcca ccccatcatt cgccaatagc
     4081 actaaacaac gcgaaccgcc ttgccagttc caaggctcct ctcgattgcg tcaaggatca
     4141 gtacgggttc cagaagttca ttcctctcaa gcggtctttt ccccgtcctc accatcgcta
     4201 gaactatctc cagtccgtct ttgtaacagg ttcccgcatc caaagcccgg ccaacatgcc
     4261 cctttttccc gaaggcaagc atgtgaaaca cgtacgcggc atcacccagg aaattcagga
     4321 ctgtcacgcg cccgtcttcg tacatgagcg ttgcaacgat gtttttcttg ttttccaccg
     4381 cgtgaacaga aatcacaccg gggccgttca gtgcctgcgc aagctccacc gtgtggatgc
     4441 cgtagaaggg aagtccaccg tactcgctct ggcggtccgc cggtccgctg aacacggaga
     4501 gcgacacagg gccgacctct tccgcttcct tgatgaacgc cagcatatcc cgcgaatacc
     4561 gtagcgtcga gaacgaggtc aagggtgtgt ttttcgcttc cgcgagatcc agaagcgcgc
     4621 gcgcgtcatc tggagaaatg gcaagcggtt tgtcgacaaa aaccgggatg ccagcccgca
     4681 ggaatggttc cgcatactga cgatgcagtc caccgtggcg gaacaccacc agcactgcgt
     4741 ccacggaacc gatcatttcc tccggtctgt ccacaatacg aggaacattg ccgagagtcg
     4801 caacttcctt ggtccgtgcc gggtcgacgc cgaatatcgc aacgactttt gcatcggcaa
     4861 ccgtcaggtc ctgcggaaca ttgcagagtt tgctgaacgc ttcggcgtgg gaattatctg
     4921 agccaacaat cccgatacga accatgtgtg cgtcctccac gaggtgttcc atcgcgcttt
     4981 tcgagtctgt tccggggaaa attcgaccga cactgcaagc ccgcgcgatc aggggatgag
     5041 ccccatatcc ttaaggccca cggtcagttt gtgggcttcc gtatcggtca gttcacgatg
     5101 aggcggtcgg acagttccta tgtctattcc acgaaggcgt aacgcagtct tgtacgttgc
     5161 cagatagcgg ccggacatca ggattttcgt tagcgccgtg agcctttcct gagcctcttc
     5221 cgcgcgccgc atatcccccg cgttgaacgc gtcgtacacc gcctgaaata cttccgggaa
     5281 cgcgttggcc gtcgatgtca cgcttgcttt ggcacccaaa cggagggcgt ccaacgttcg
     5341 atggtccgct ccgcttatta cctggaaatc tggaagcccc atttcgagtt catttttcat
     5401 ccgttccaga tcaccggaag aatccttgat acccttcagg tg
//
LOCUS       DAOTNG010000214         5440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12636_length_5440_cov_4.849582, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000214 DAOTNG010000000
VERSION     DAOTNG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12636_length_5440_cov_4.849582"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5179_10230"
     CDS             complement(<1..441)
                     /locus_tag="P5179_10230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362672.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRS95637.1"
                     /translation="MPVPLIFDVSKPGRVGHSLPACAVPAKPTESLIPAKFLRSAPPR
                     LPEVSEGEAVRHFVNLSALNHHIDRGMYPLGSCTMKYNPKVNEVAARLSGFAEMHPLA
                     PPQTTQGLLRLIHELERMLCEITGFAGVTLQPPAGAASELTGLLV"
     gene            complement(527..1291)
                     /locus_tag="P5179_10235"
     CDS             complement(527..1291)
                     /locus_tag="P5179_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95638.1"
                     /translation="MVAHILEYSAIIGTLVLMEGLLAADNALVLAVLVSRLPDRNEQK
                     KALLYGIIGAFVFRALSLVSAYWLIRMWEFKALGAAYLIFMTARYFWSGEYRTDRHAE
                     PRPFPAAKETEREGGEPAGARARFSRRFWPTVAMVELTDLAFSVDQIVAAVGLSSHLA
                     VVYTGGMIGIIAMRFAAGGFLLLIERHPKLAYTGHILPAWIGLKLAMETLSNHPFDWH
                     VKMPAWVFWSGMAALVAWGLLSRGRTDAASHEIQTK"
     gene            complement(1376..2011)
                     /locus_tag="P5179_10240"
     CDS             complement(1376..2011)
                     /locus_tag="P5179_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS95639.1"
                     /translation="MSQPFLPLWHIETDDPVYALTFDDGPHPEITPKLLDVLDRKGVQ
                     ATFFLLGERALEYPGLVAQIAERGHGIGSHGFRHSWNWWRTPDTLARDLDRSERILAG
                     VLREPKLCRPPYGMASPQWLSAARGHGYRTVLWSLGSGDWKYTSPGRIVRRVRRRLRP
                     GAIVLFHECRAETGNGYRHTLEAVSQLLEEESVLRPANLHKLIAKESMLVP"
     gene            complement(2008..3018)
                     /locus_tag="P5179_10245"
     CDS             complement(2008..3018)
                     /locus_tag="P5179_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS95640.1"
                     /translation="MPRDKRPILTVSAPPMAESSRSRWLKRLRLAFTIIVFAVLVVSV
                     DPVAIAATWRRIDPQWISLAVLLVPPNLFCQYRRISAALMPVYPAISFRTAIHPLLAG
                     LAMGAVTPGRVGELGQVMLLPSGGRTKALGTLTVMRAYGFLSVVALGLAAWMANPALL
                     RTDSRTAVCTGGAVLLVLAALTGVAEWYFRREEHLTTGGLIRRIRGAEGVFAGVKTLS
                     AKDRVRFCGWSLALSFVYLTQLTFLMRAFGAAVPLWDGLLAGAVTTGIVAVLPVAVGN
                     IGVRETAAVVVWKQVGTNAAAAFNGAFLLFLINVVLPGLAGIIWNWWETFRGEGSPSR
                     RP"
     gene            complement(2985..4082)
                     /locus_tag="P5179_10250"
     CDS             complement(2985..4082)
                     /locus_tag="P5179_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95641.1"
                     /translation="METCRQLISYIGPGAPATRRPGTGNEPFLRPEIGFTPRWYRQAL
                     GIDFGERWHTDPAYRREAIIAMRGELRRRFPGTAIGGIDRPDEPLDLLTGTYGASTVA
                     AIFGVSIVYSSDNWPNCEHRYLSDDEMDAITPPDLNSNPHFQALMDQVEWIAATEGRV
                     VGYINWQGVLNNAQRLRGEQLFIDMVEEPERCARLFDAVTTTMIDAAKRLHARQSQSG
                     VDVRFFTVSNCLVNMVSSRAYREQLLPFDRRISEAFCCIGIHNCAWNANPYLDPYAEV
                     PRVAYIDMGIMSDLERARRLFPDARRALMYTPMDVADKSLDQIRSDFEHVADTYGPCD
                     IVLADIEAGTPDERVLSLVEICRVISARFSR"
     gene            complement(4117..4824)
                     /locus_tag="P5179_10255"
     CDS             complement(4117..4824)
                     /locus_tag="P5179_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95642.1"
                     /translation="MRVSFLSACVLPGSLLFCAGCAGLLERYAIVENSPVVVLYQDAD
                     AHAARQNYPAALGAYREVIRRYPGSAYAEASYLRIASLYLDRAAPERGWSGWEGADYV
                     AARDTLMALLEKFPRTQYGQEARNWIKVLDMALDVDSRTEIVRDTVTVQVPVITADTS
                     DSSAAWQSERARLQRTINAITAERNRFRTQRDTCRAGMERLRAENADLDAQLRRTKSE
                     MERMRRMLVDLERRSGN"
     gene            complement(4844..>5440)
                     /locus_tag="P5179_10260"
     CDS             complement(4844..>5440)
                     /locus_tag="P5179_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95643.1"
                     /translation="GGSDLVSSQGAIVERPTHEEHLILEATLFSDTLVAADIARICEY
                     DSSGSRCDSIAIAYKARHQIPEQFRIELAALANLSAPFRLEPLTIYLTDEDGIDYEPR
                     RKIFSTPVTAQRTYLDREVTRYDPYTSWEYQVYQYTAGYEFQSTGRATLYFDRVNIIG
                     KDLLGPGAKLTLKFRQNRTELGKITWDLDAIRAGSTAR"
BASE COUNT         1047 a         1682 c         1725 g          986 t
ORIGIN      
        1 cacaagaagt ccggtgagtt cgcttgccgc gccggcaggg ggctgaagcg tgacaccggc
       61 aaacccggta atttcgcaca acatgcgctc cagttcatga atcagccgca ggagtccctg
      121 cgttgtctgc ggaggggcga gcgggtgcat ttccgcgaaa cccgaaaggc gggcggccac
      181 ctcgttcact ttcgggttgt acttcatcgt gcacgagccg agaggataca ttccgcggtc
      241 aatgtggtgg ttaagcgcag agagattcac gaaatgccgc actgcctccc cttcgcttac
      301 ttcgggcagg cgtggcggtg ccgaccgcag gaatttggcc gggataaggg attccgtcgg
      361 tttcgcgggg accgcgcagg caggaagact gtgtccgacc cggccgggtt tgctcacgtc
      421 gaaaatcagt ggcacaggca tgaaagaacg actccttgat cgcgaacgag tggaaaaaag
      481 tcttccttaa gcgtacgaat gtgatgcgga agccgcaaga aacggttcac ttcgtttgga
      541 tttcgtggct cgcggcgtca gtcctgccgc ggctcagcaa tccccacgcg acgagggccg
      601 ccatcccgga ccagaatacc catgccggca ttttcacgtg ccagtcgaag gggtggttgc
      661 tcagcgtttc catcgcgagt ttcagaccaa tccacgcagg aaggatgtga ccggtgtatg
      721 cgagcttcgg gtgccgctcg atgagaagca gaaatccccc cgcggcaaac cgcattgcga
      781 tgattccaat cattccaccc gtgtagacca ctgcgaggtg actggaaagc cccaccgcag
      841 ccacaatctg atcaacggaa aacgcgaggt ccgtgagttc gaccatggcg acagtcggcc
      901 agaaccggcg actgaaacgg gcccgtgcgc ccgccggttc tcctccctcg cgttcggttt
      961 cttttgccgc gggaaagggt cgcggttccg cgtgccgatc cgtccggtat tcgccggacc
     1021 agaaataccg ggccgtcatg aagatcagat aggccgctcc cagggctttg aattcccaca
     1081 tacggatgag ccagtaggcg ctgacaaggg agagcgcgcg gaacacaaac gccccgatga
     1141 taccgtaaag gagtgccttt ttctgttcgt tcctgtccgg aagccgggac accagtacgg
     1201 cgagcacgag cgcgttgtct gcggcaagaa gcccctccat aagcaccagg gtgccgatga
     1261 tggccgagta ttcgaggatg tgcgctacca tatcgtgtcc ggacgggaac tgtgcggggc
     1321 cgcaggtgcg cggaaagagt tgagatgtgc cacagaaagc tcacgaggac ggggtttacg
     1381 gaaccagcat cgattccttt gcgatcagtt tgtgcaggtt cgcggggcgg aggacagact
     1441 cctcttccaa aagctgcgag accgcctcca gcgtgtggcg gtagccgttc ccggtttcag
     1501 cccggcattc gtggaacagc acgatggcac cgggccgcag gcgacgccgc acgcggcgga
     1561 cgatccttcc gggggaggtg tatttccagt ccccggagcc caggctccag aggacggtgc
     1621 ggtacccgtg cccacgtgcg gcggacagcc actggggact cgccatgccg tacggcgggc
     1681 ggcaaagctt cggctcacgc agaacgccgg ccagaatgcg ctcggatcgg tcgagatcgc
     1741 gcgcaagcgt gtcgggtgtt ctccaccagt tccacgagtg cctgaacccg tgcgaaccga
     1801 tgccgtgccc gcgttcggcg atctgtgcga ccagccccgg gtattccagt gcccgctcgc
     1861 ccaggaggaa gaacgttgcc tgaacgcctt tgcggtccag cacatccagc agcttcgggg
     1921 tgatttcggg gtgcgggcca tcgtcgaacg taagcgcgta caccgggtcg tctgtttcga
     1981 tgtgccacag cggcaggaat ggctggctca tggccgcctc gaaggggagc cttccccgcg
     2041 gaatgtctcc caccagttcc agatgatccc cgccaggccg ggaagcacga cgttgatgag
     2101 gaacagcaga aatgcgccgt tgaacgctgc cgccgcgttc gtacccacct gtttccagac
     2161 caccaccgcg gccgtttccc tcaccccgat gttgcccacg gcaacgggca gcactgcgac
     2221 gattcccgtg gtgacagcgc ccgcaagcag gccatcccac aggggcacgg cggcgccgaa
     2281 cgcgcgcatc aggaacgtga gctgtgtcag gtagacgaag gacagcgcaa gagaccatcc
     2341 gcagaaacgc acgcgatcct tcgcggaaag tgtcttcacg cccgcgaata ctccctccgc
     2401 tccccggatc cggcggatca gcccgccggt ggtaaggtgt tcctcgcggc ggaaatacca
     2461 ttcagcaact ccggtcagcg ccgccagaac gagcagaacc gcgccgccgg tacacaccgc
     2521 cgtacgcgaa tcggtgcgca acagagcggg gttcgccatc cacgcggcga gaccgagagc
     2581 aacgacggaa aggaacccgt aggctctcat cacggtcagc gtaccgaggg ccttcgtgcg
     2641 cccaccggaa ggcaacagca tgacctgccc cagttcaccg acgcggcccg gcgtgacggc
     2701 gcccatcgca aggcccgcga gcagggggtg aatcgccgta cggaatgaaa tcgccgggta
     2761 caccggcatc agcgccgcac tgattctgcg gtactggcag aagagattgg ggggaacaag
     2821 gaggactgcc agggatatcc actgcggatc aattcgccgc cacgtcgccg cgatggcaac
     2881 ggggtccacg ctgacgacca gaaccgcgaa cacgatgatc gtgaaggcga gtctaagcct
     2941 tttcagccat cgcgagcggg aggattccgc catgggaggt gcgctcaccg tgagaatcgg
     3001 gcgcttatca cgcggcatat ctccaccagg gagaggacgc gctcatccgg cgtgccggct
     3061 tcgatgtcgg caaggacgat atcgcagggc ccgtacgtgt cggcgacgtg ctcgaaatcg
     3121 ctgcggatct ggtccagaga tttgtccgca acgtccatcg gggtgtacat gagtgcgcgt
     3181 cgggcatcag ggaacaggcg tcgtgcgcgc tccagatccg acatgatgcc catatcgatg
     3241 tacgccaccc gtggcacctc ggcgtacggg tcgaggtacg ggttggcgtt ccacgcgcag
     3301 ttgtgaatac caatgcagca gaacgcctcg gaaattcgtc tgtcgaaggg gagcagttgt
     3361 tcgcgatagg cccggctgga aaccatgttc acaaggcagt tgctgacagt gaagaaacgg
     3421 acgtccacgc cggattggct ctggcgcgcg tgcagacgtt tggcggcgtc gatcattgtg
     3481 gtggttacgg cgtcgaacag gcgggcgcag cgttccggtt cctccaccat atctatgaac
     3541 aactgctcgc cgcgcaggcg ctgtgcgttg ttgagcactc cctgccagtt gatgtagccc
     3601 accacccgac cctccgtggc ggcgatccat tccacctggt ccataagcgc ctgaaaatga
     3661 ggattgctgt tgaggtcggg cggcgtgatc gcgtccatct cgtcgtcgga gagataccgg
     3721 tgttcgcagt tgggccagtt atcgctcgaa tacacaatcg acacgccaaa aatcgccgcc
     3781 acggtggaag ccccgtaggt tccggtaagc agatccagag gctcatcggg ccgatcgatg
     3841 ccaccgattg ccgtcccggg aaaccggcgc cgcaattcgc cgcgcatggc gatgatggct
     3901 tcacgccggt atgcggggtc ggtatgccac ctttccccga aatcgatccc aagcgcctgt
     3961 cggtaccacc ggggagtgaa accgatttcc gggcgaagga acggctcatt ccctgtaccg
     4021 ggccgccgcg tggcgggcgc tccggggccg atgtagctga tcaactggcg gcatgtttcc
     4081 ataggtgtga ccccccccgg tgcgagaaat ccgcggtcag ttgccggacc ggcgttccag
     4141 atccacgagc atgcgccgca tgcgctccat ctcggacttc gttctgcgca actgagcgtc
     4201 caggtcggca ttttccgcgc gcagccgttc catgcctgcc ctgcaggtat cccgctgggt
     4261 gcggaaccgg ttgcgttccg cggtgattgc gttgatcgtt cgctggaggc gggcgcgttc
     4321 gctctgccac gccgcggaag aatcggacgt atcggcggtg ataacaggaa cttgtacggt
     4381 aaccgtatcc ctcacgattt ccgtacggga atccacgtcc agcgccatgt cgagcacttt
     4441 gatccagttg cgcgcttcct gcccgtactg ggttcgagga aacttctcca gcaacgccat
     4501 cagcgtatcc cgtgcggcca cgtaatcggc gccttcccag ccgctccacc ctcgctcggg
     4561 agcagctcgg tcaaggtaga gggaggcgat gcggagatac gatgcttccg catacgcgct
     4621 cccggggtac cggcgaatca cctcccggta ggcgccgagc gccgccgggt agttctgccg
     4681 cgccgcgtgt gcgtcggcat cctggtaaag caccaccacc ggcgagttct cgactatggc
     4741 gtaccgttcg agtaatccgg cgcatccggc gcagaacagc agggatcccg gcagaacgca
     4801 ggcagacagg aatgaaacac gcatcgttcg agattcgctt tcctcaccgc gccgtgctgc
     4861 ccgcccgtat ggcgtccaga tcccacgtga ttttccccag ctccgtgcgg ttctggcgga
     4921 acttgagcgt gagtttcgct ccgggcccga ggagatcctt cccgatgatg ttcacgcggt
     4981 cgaagtagag cgtcgctcgt ccggtggatt ggaactcgta cccggcggtg tactggtaca
     5041 cctggtattc ccacgaggtg tacgggtcgt acctcgtcac ctcgcggtca agataggtgc
     5101 gttgcgctgt caccggcgtg gagaagattt tccggcgcgg ttcgtaatca attccgtcct
     5161 catcggtgag gtaaatcgtc aaaggttcga ggcggaaggg tgcggataga ttcgccagcg
     5221 ccgccagctc gatacgaaac tgctccggga tctggtgtcg cgctttgtac gcaatggcga
     5281 tgctgtcgca ccggctgcct gaggaatcgt actcgcagat gcgggcgata tccgccgcca
     5341 ccagcgtgtc ggagaacagg gtggcctcca ggatcaggtg ctcttcgtgt gtggggcgtt
     5401 ccacgatggc cccctgcgag ctcacgagat cgctcccgcc
//
LOCUS       DAOTNG010000215         5432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12660_length_5432_cov_5.079412, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000215 DAOTNG010000000
VERSION     DAOTNG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12660_length_5432_cov_5.079412"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="P5179_10265"
     CDS             complement(<1..967)
                     /locus_tag="P5179_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95644.1"
                     /translation="MKLRIPLMSLAFCAAVSAKPAHGYVLRAPDGTPAAVCGTMLLRT
                     DPSRSRPAARVTLPGSYVSPARWFRIHYTTASGSADAVPAADANQNGVPDWVELAASV
                     ADSVYANYRALGYTTPLTDGGASGGTEYDIFFINLAPQNVYGYTFSPPDGYMELDNDY
                     AERIYQTKGATGLRITLAHELFHSVQFAVWVGGNDGIWWQEATATFMEEYNYPDINDY
                     WQYLDPDWFCNTLFENPATPLNQGSTGSCDTHMYGAAVFCQYLVRTDSVSGHAGIRYS
                     FDRQKVLRSSAPNVVVDALETTMQQPMRELIAGFWSRAYFTGPRSP"
     gene            1219..2352
                     /locus_tag="P5179_10270"
     CDS             1219..2352
                     /locus_tag="P5179_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95645.1"
                     /translation="MTPKQRVFTALRHEEPDFVPIGEFAIDYPAFEAVLGRPTFWRGH
                     FKTMKALWEGRRDEVVESMIRDVIEFYHKTDQDIIPVSLVPSRNKEIIPWEEIAPMTF
                     RDPRGDVYRVSEKSQWLMRIKYAEPAREYSVEDFPVPTEFTEPDESEWELVRAVVKEF
                     GATHFILARSGDGTFPLIGGMERGLILIAEHPDVAEAAAKAAVARTMYMDGLWAKEGV
                     DGLCPGEDYADSRGPLVSPRVIRERFFPSMAAHVAHAHGLGLPVLKHACGNNWKIMDM
                     LVEAGYDAYQSIQESATMDIIRLKREYGDKITLWGGVGTETLIAGSTEDVRNEVRRAV
                     RHCAPGGGFLLGSSHSILIGAQPDNYLAMLDEARKVGRYPVTI"
     gene            complement(2373..3503)
                     /locus_tag="P5179_10275"
     CDS             complement(2373..3503)
                     /locus_tag="P5179_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRS95646.1"
                     /translation="MKYKASTVQAFLQRIVESLDAQIQHIETASPQTFYGNVFSEIPE
                     ATYWVVTGKGRVRHIADKISASLASTGTPSYFVDAANEAHGLPLLSPSSRVLLLWRRY
                     QPVADLTRLLEAIPASVAVDIVTDVTDLPVNRAGNLFRIVGVESSALEVPNDIVALAM
                     GDSCVAGLESARKFTELDFLKDHPKGNLGKTIAAAQSAREISYLPPPVQAFAALKEER
                     EGVRNLLEGLDAAAVTEWLDVFSRPWERIITTGMGKPGYCMRYLAFLLNELGRSAFYL
                     HPAEGVHGDLGRIGERSLVIAYSHSGETDEVLNILPRMKERGATLLALTNNTASTMAK
                     AADAALKAGAPEVDPIRKAPTGSTTAALSLATALVCSDRPAR"
     gene            complement(3563..4594)
                     /locus_tag="P5179_10280"
     CDS             complement(3563..4594)
                     /locus_tag="P5179_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRS95647.1"
                     /translation="MKQGLILGCGSVVVDVLKLVPNFKVGDKTKIIEQDGRSKKLIAG
                     GVTLNNLVWSRMLGARSGVFGRLGGDEYGKFLLKEMDRFGVAHDQLHVIEGEEAAYTD
                     FFLDRQPERTLFMFFGTTATTTPEFIRTHCADAIRNAAYVVTEISQLPLDTVLEIEKI
                     AHEANVPVAVDLDIAPSNSIMQYIGTAAQYEKVARGATVLKPCDAAATELAGTDDLEK
                     AIQKIHRDFGNHRLVAITAAAKGSLLYDGKNVHWENVLDLSKVGRKLVDPCGAGDAYF
                     GGLNAALNLLGFDAPLADIGTIANATAGICCSEYGAFPVDPETPRAAIKNLIAANRGG
                     ATADRLIPA"
     gene            4942..>5432
                     /locus_tag="P5179_10285"
     CDS             4942..>5432
                     /locus_tag="P5179_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS95648.1"
                     /translation="MVLVIGLNPAIDRILIVPNFAVGATLKAESVQTLAAGKAMNVAA
                     VLRLLGVRVGYAGFVGREEAAFFRRRLPDVECWMVEVDGRTRTDTTLIDPVGHTETHV
                     REPGFAVQTEDMERFLDAFTGVIVGVEFVVMSGSLPPGAPEGLYAELVGLCHARHIRT
                     LLDT"
BASE COUNT         1071 a         1577 c         1642 g         1142 t
ORIGIN      
        1 ggggggaacg tgggccggtg aagtacgccc gcgaccagaa cccggcgatc agctcgcgca
       61 tgggttgttg catggtagtc tccagtgcgt caaccaccac gttcggcgcg ctggatcgca
      121 gcaccttctg ccggtcgaac gagtagcgga ttcccgcatg gccgctcaca gaatcggtac
      181 ggacgagata ctggcagaag acggcggcac cgtacatgtg ggtatcgcac gacccggtag
      241 acccctggtt caacggcgtc gccggatttt cgaacagcgt gttacagaac cagtcggggt
      301 ccagatactg ccagtagtcg ttgatgtccg gatagttgta ttcctccatg aacgtggccg
      361 tcgcttcctg ccaccagatc ccgtcgttcc cgcctaccca gaccgcgaat tgaacagagt
      421 gaaaaagctc atgcgccagc gttattcgga ggccggtcgc gccttttgtc tgatagatcc
      481 tttcggcgta atcgttgtcc agctccatat acccatcggg cggagagaac gtatacccgt
      541 acacgttctg gggggcgagg ttaatgaaga agatgtcgta ctcggttccg ccagatgcac
      601 ctccatcagt caacggagtg gtgtacccga gcgcccggta gttggcatac acggagtccg
      661 ctacgctggc ggcaagttcc acccaatccg gtacgccgtt ctgatttgcg tctgccgcgg
      721 gtaccgcatc cgcgctgccg ctcgcggtcg tgtagtggat tctgaaccaa cgggcgggac
      781 tcacgtacga accggggagc gtcactcggg cggcggggcg tgatcgcgaa ggatccgttc
      841 gcagcagcat ggttccgcac acggcagcgg gcgtaccgtc cggtgcacgg agtacgtagc
      901 cgtgcgccgg ttttgcgctg acggccgcgc agaacgcaag ggacatcaaa gggattctga
      961 gcttcatgct atggaccagg gttgcagcaa ggtcgcctgt tctacaagaa agtacaccga
     1021 aaaaggcgga cttctccgtg accaatatca cttagaagaa gcaacccgcg taccgagaac
     1081 ggcaaacgca ctgccgacgc ctttcgaaag aacggctcct gcgcccaggg gctgccgttc
     1141 cgctcgatgg ttcacgttgg tagattgtgt ggtgcctttc ccgttttgcc aaccacactc
     1201 gttgcctgga atgaacggat gacaccaaaa caacgtgttt tcacggcgct acgccatgaa
     1261 gaacccgact tcgtccccat cggggaattc gccattgact accccgcctt tgaggccgtg
     1321 ctcggaagac caacgttctg gcgcgggcat ttcaagacaa tgaaggccct ctgggaagga
     1381 aggcgcgacg aagtcgtgga gagcatgatc cgcgacgtca ttgaattcta ccacaaaacc
     1441 gaccaggaca tcataccggt cagcctcgtt ccaagcagga acaaagaaat tataccctgg
     1501 gaggaaatcg cgcccatgac cttccgggac cccaggggcg acgtctacag agtctcggaa
     1561 aaaagccagt ggctgatgcg gatcaaatat gccgaaccag cgcgtgagta ctcggttgaa
     1621 gatttccccg ttccgacgga gttcacggag cctgacgagt ccgagtggga acttgtccgt
     1681 gcagtagtga aggaattcgg cgccacccat tttattctag cgcgaagcgg ggacggtacg
     1741 ttcccactta tcggcgggat ggaacgcggg ctcatcctga ttgccgagca cccggatgtt
     1801 gcggaggctg ccgcaaaggc ggctgttgcc cgcacgatgt acatggacgg tctctgggca
     1861 aaggaaggcg ttgacggatt gtgtcccggt gaggactacg ctgattcccg cggccccctc
     1921 gtgtcgccgc gcgtgatcag ggagcgcttc ttcccttcaa tggccgcgca cgttgctcac
     1981 gcccacggcc ttggtctgcc cgtgctcaaa catgcgtgcg gcaacaactg gaaaatcatg
     2041 gacatgctcg tggaggcggg gtacgacgcg taccaatcca tccaggaatc ggcaacgatg
     2101 gatatcatcc gcctgaaacg ggaatacggg gacaaaatca ccctgtgggg aggcgtaggc
     2161 acggagacat tgattgccgg ttcaacggag gatgtgcgca atgaagttcg ccgcgcagta
     2221 aggcactgcg cacccggcgg aggcttcctg ctcggttcca gccactccat tttgattgga
     2281 gcacagccgg acaactacct ggcgatgctc gacgaggcga ggaaagtcgg ccgctatcct
     2341 gttacgatct gattccgccg tgctgtgccc tgctacctcg ccggtctatc ggaacacacg
     2401 agggccgtcg cgagcgacag cgccgccgtg gtggaaccgg tgggcgcctt ccgtatcgga
     2461 tcgacctccg gtgctcccgc cttcagcgcc gcgtccgccg ccttcgccat cgtgctggcg
     2521 gtgttgttcg taagcgcaag aagcgttgct ccccgttctt tcatccgcgg cagaatgttc
     2581 aatacctcgt ccgtttcgcc gctatgggag tacgcgatga cgaggcttcg ttcgccaatc
     2641 ctgccgaggt ctccgtggac accttcagcc ggatgcaggt agaacgcact gcgtcccagt
     2701 tcgttcagaa gaaaggcaag ataccgcatg cagtaaccgg gtttccccat gcccgtggtg
     2761 ataatgcgct cccacgggcg tgagaacacg tccagccatt ccgttacggc cgctgcatca
     2821 agtccctcga gcagatttcg taccccttcg cgctcttcct tcaacgcggc aaacgcctgc
     2881 accggtggcg gcaggtacga tatttcgcgc gcgctctgcg cggcagcgat tgtcttccca
     2941 aggttgccct tgggatggtc cttgaggaag tccagctccg tgaatttccg cgcactttcc
     3001 agcccggcaa cacacgaatc gcccatggcg agcgcgacga tgtcgttcgg cacttcgagt
     3061 gcgctcgatt cgacgccgac aatacgaaaa agattcccgg ccctgttcac ggggagatct
     3121 gtcacgtcgg tcacgatgtc aaccgcaacc gaagcgggga ttgcctcgag cagccgagtc
     3181 agatcggcaa ccggttggta tcgccgccag agcagaagaa cacggctgga agggctcagc
     3241 aagggcaacc cgtgcgcttc gttcgctgca tccacgaagt acgagggcgt tccggtgctc
     3301 gcgagagaag cggagatttt gtctgcaatg tgccgcacgc gcccttttcc cgttacaacc
     3361 cagtatgtcg cttccgggat ttcggagaac acgttgccgt agaatgtctg tggtgaggca
     3421 gtctcgatgt gctgtatctg cgcatcgagg gattctacaa tgcgttgaag gaacgcctgt
     3481 acggttgaag ctttgtattt cacgggattt cctcaaaagg atgggcccgc gaggtgattc
     3541 tgctcacccc gcgggcagtg cgtcacgcgg gaatcaggcg gtctgccgtt gccccccctc
     3601 tgttggcggc gataaggttt ttgatcgccg cccgcggcgt ttctgggtca accgggaatg
     3661 cgccgtattc agaacagcag atgcccgcag tggcgttcgc gattgtcccg atatctgcga
     3721 ggggggcgtc aaacccaagc aggttgagcg cggcgttgag accgccaaaa tacgcgtctc
     3781 ctgcaccgca cgggtcgacg agtttgcgtc cgactttcga gagatcgagc acgttttccc
     3841 agtggacgtt tttcccgtcg tacagcagac ttcctttcgc cgcggccgta atggctacca
     3901 gtctgtggtt gccaaagtcg cgatggatct tctgaatcgc cttctcgagg tcatcggtac
     3961 ccgccagttc agttgcagcg gcgtcgcacg gtttgagaac cgtagcgccg cgtgccactt
     4021 tttcgtactg ggccgcggtt ccgatgtact gcatgatgga attgctcggg gcgatatcca
     4081 gatcaaccgc gaccggcaca ttcgcttcgt gcgcgatttt ctcgatttcc agtactgtgt
     4141 caagcggaag ctgggagatc tccgttacga cgtatgccgc attgcgtatg gcatccgcgc
     4201 aatgcgtgcg aatgaactca ggtgtggtgg tcgccgtggt tccgaagaac atgaaaaggg
     4261 tgcgttccgg ttgccggtcg aggaagaaat cagtgtaggc ggcttcttcg ccttcgatca
     4321 cgtgaagctg atcgtgtgca acgccgaacc gatccatttc tttcaacagg aatttcccgt
     4381 attcgtcccc gccgagccgc ccgaacactc ccgaccgggc gcccagcatg cgtgaccaga
     4441 cgaggttgtt gagcgtcacg ccgccggcga tgagcttctt cgagcgacca tcctgttcga
     4501 tgattttcgt cttgtcgccg actttgaaat tcggaacgag tttgagcacg tcaacgacca
     4561 cacttccgca gccgagaatc aagccctgtt tcatcgtttc cctcgttggt ttctggttgc
     4621 cgttcctacg tgccgaaccg gccgtgatgg ggccggcgca gggaatagtt ctccaccgtt
     4681 cgggtgactc aggagaccac gaaatccggg cgaaccggtc gtccccggct ttgtaccgga
     4741 ctggatacag accgccatcg aggggtctgg cacctgcagc gggaaaacgc cggtgttcct
     4801 gccagtcatc gcgttctctg taatgatact cgaatgcggg gatcggatga aaccatgcat
     4861 gccgcgcttt gcccgcgcag aatgtcgtcc tacctttctc tcacgtatcc gctttaacaa
     4921 cccgtcgcgt ccggttttcc catggttctg gtaatcggtc tgaatcccgc catcgataga
     4981 atcctcatcg ttccgaactt cgcggtggga gcaactctga aagcagaatc ggtgcagacg
     5041 ctggccgccg gcaaggcgat gaatgttgct gcggttctgc ggctgctcgg ggtacgagtg
     5101 ggatacgcgg ggttcgtggg aagagaagaa gccgctttct ttcgcagacg cttgcctgat
     5161 gtggaatgct ggatggtgga agttgacggt cggacgcgga ctgacaccac gctaattgac
     5221 ccggtgggtc atacggaaac tcacgtgagg gagccgggat ttgcagtcca gaccgaagac
     5281 atggaacggt tccttgacgc gttcacggga gtcatcgttg gcgtggagtt cgtggtgatg
     5341 tcggggagcc tccctccggg ggcccctgag gggttgtacg ctgaattggt ggggttgtgt
     5401 cacgcgagac acattcggac attactcgac ac
//
LOCUS       DAOTNG010000216         5387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12779_length_5387_cov_5.725994, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000216 DAOTNG010000000
VERSION     DAOTNG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12779_length_5387_cov_5.725994"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="P5179_10290"
     CDS             <1..355
                     /locus_tag="P5179_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95649.1"
                     /translation="VVNFHLTAREKPPFFTTPVDLDSRLRGNDSSCRRGVQFASCTKA
                     NFGISCCASIASRAFPDSLRAQSAAGTQNHSCGTSDIRHFHRLSHPRALRSRFVPVSR
                     CTQAVIPAGPTDPNA"
     gene            complement(475..1491)
                     /locus_tag="P5179_10295"
     CDS             complement(475..1491)
                     /locus_tag="P5179_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95650.1"
                     /translation="MRRRGFAEEVLQTHSGETKMDWATIKGFNYQPSWGSSGFEIWRN
                     FDAERMGHELALGRLYFPRMNAIRLWLSHDAWTRSPEKFETAFSAALRVAEKHSLRVM
                     PVLFNRWHDATLDYGGVYLDHFLPKASWVQARDRTAEFAESVFAAHAADNRIFAWDLC
                     NEPFSYGCAPAEIPEIVKAEFAWLEKTYRIGKQCGVKAPITVGIHPGHGIEGIRQIEP
                     ISDILSIHPYWTFDSPPHDKGVYEKLLDDYVRFAERAGKPLLATETCWGATDDAKRVE
                     IIRYTLGELKKRRIGWLVYVLHHSLIADAHRIEFGPLSTPGNLAFIEADGTLRPGHEV
                     FNEF"
     gene            complement(1488..2435)
                     /locus_tag="P5179_10300"
     CDS             complement(1488..2435)
                     /locus_tag="P5179_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRS95651.1"
                     /translation="MSVLYAPWGVVYSPGYALDMSGQESVHPFDVRKYMKIVDLLHAD
                     GLLDGGAIIEPDLLTEQEAALVHTPDYMRLWTDPETMAEIFEVDYLRNVSPDTIRERY
                     LPPYFAMAGGTVRAARDALKCGTVFNIGGGFHHAHADHGSGFCFVADVAIAIRVLMRE
                     KLLQRAAIVDCDVHQGDGNATIFAGDPSVFTLSFHQEDIFPFEKATSSRDVPFPAGTG
                     DEAYMALLREHVPDVLDTHRPDIVFFVAGSDVHADDPLAATELTSAGIIQRDAFVWSE
                     TRKRGIPFVMVLAGGYFPRCPEVHAASIGNIIRKARKQV"
     gene            2748..4178
                     /gene="amrB"
                     /locus_tag="P5179_10305"
     CDS             2748..4178
                     /gene="amrB"
                     /locus_tag="P5179_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRS95652.1"
                     /translation="MNTQAPIRTAAVAGQFYPASPDVLREEVDALLAAAPKDDRITGD
                     IVAIVAPHAGYIYSGRIAAIAYRQVQGREYDAVVILAPSHREAFAGVSLMPAGGYETP
                     LGIAGIHEGIARKLLEQGGCIHETRKGHGPEHAVEVQIPFIQRALGSVPIVPLVMGEH
                     SWPVCNSVARAIARAVEGMNVLVVASSDLYHGDSEAECETIDRVTLAAAESRPPQQFC
                     ADLDAGRAQACGGAPIAVAKEYARLLGASGVRVLAHATSADVTGHRGGYVVGYGAIAY
                     FRNGAAVEQSSLSEEDRALLLRLARESVRAAAAGRPLPQPPKGHPALEEPKGAFVTLH
                     KDGDLRGCIGLLQAYQPLAATVIEMAQAAAINDPRFPPLREHELDTTALEISILSPFE
                     VTRPEHVEVGKHGIFITGRGRRGVLLPQVATEQGWDRETFLEHVCLKAGLPRNAWQSS
                     DVVLERFSAEIIHDTGPIGREETRRN"
     gene            4188..4883
                     /locus_tag="P5179_10310"
     CDS             4188..4883
                     /locus_tag="P5179_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS95653.1"
                     /translation="MPITILLVEDEPGIRSVTAKWLAHHGYAVDEAENGRAGWERVHQ
                     SPPDLVITDIAMPEMDGVELCRLIKTTESTADIPVLMFTSHAEADLQLAGTKAGADAY
                     IPKNGDLRVLNARIEALAAARSRARQETERTRRLTLSQSVTTLAHHINNSVIAIHATA
                     SVVDPNNPQQAQKLRHVCQTEARKMLLVLKALKEMAEREELKTTVYVGNELMFDLEAA
                     LRQLDSGAKSPPR"
     gene            complement(5120..>5387)
                     /locus_tag="P5179_10315"
     CDS             complement(5120..>5387)
                     /locus_tag="P5179_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95654.1"
                     /translation="GNDERKVIDFFFARGSGTRPQSVIPAEAGIQTGWPGAAFAVISI
                     HRPPTVRRTPLDDRCSGAHLPVVEWLTREMYRDASARYIRADGR"
BASE COUNT         1020 a         1747 c         1608 g         1012 t
ORIGIN      
        1 agtcgtaaac tttcatctca ccgcccgcga aaagccgccc ttttttacca cgcctgtgga
       61 cctggattcc cgccttcgcg ggaatgacag cagttgccga aggggcgtgc agtttgcctc
      121 atgcacgaag gcgaatttcg gcatttcgtg ttgcgcatca attgccagca gagcattccc
      181 ggattccctt cgagcgcaaa gcgcggcggg aacccagaat cattcctgcg gcacatcaga
      241 cattcgtcat ttccacaggc tcagccaccc acgagccctg cgttcccggt tcgttccagt
      301 ctcccggtgc acccaggccg tcattcctgc cggcccgaca gaccccaacg catagccaaa
      361 tcgtcattcc cgcgcaggcg ggagtctcgt ttccgtctgc cagcggcggc ggtcgtcgag
      421 tggcgggcgc aagacgcgct cgacgtatcg agacgacccc gctctgatcc acgctcagaa
      481 ctcgttgaac acctcatgtc ccgggcgcag cgttccatct gcctcaatga acgcaaggtt
      541 gccgggggtg ctgagcggcc cgaattcgat ccggtgtgcg tcggcgatga ggctgtggtg
      601 aagcacgtag accagccatc cgatgcgccg cttcttcagc tctcccaacg tgtaacggat
      661 gatttcgacc cgctttgcat cgtccgtcgc gccccagcac gtttccgttg caaggagcgg
      721 ctttcccgcg cgttcggcga accggacata gtcgtccagc agtttctcat acacaccctt
      781 gtcgtgcggc gggctgtcga acgtccagta cgggtgaatg ctcaggatat cgctgatggg
      841 ctcaatctgg cgaatgcctt ctatcccgtg tcccggatga attccgacgg tgatcggagc
      901 cttcacgcca cactgcttgc cgatccggta cgtcttctcc agccacgcaa actcggcctt
      961 gacgatttcg ggtatctccg ccggtgcgca tccgtaggaa aacggctcgt tgcagagatc
     1021 ccacgcaaag atgcggttgt cggcggcgtg agcggcgaaa acggattccg caaactccgc
     1081 cgtgcgatcc cgcgcctgaa cccagcttgc ctttggcagg aagtggtcga gatagacccc
     1141 tccgtaatcg agcgtggcgt cgtgccatcg gttgaacagg accggcatca cgcgaaggga
     1201 gtgtttttcc gcaaccctta acgccgcact gaacgccgtt tcgaacttct ccgggctgcg
     1261 tgtccatgcg tcgtgcgaaa gccacagcct gatggcgttc atgcgtggga aatacaacct
     1321 tcccagcgcc agttcgtgcc ccatgcgctc cgcgtcgaag tttcgccaga tttcaaaccc
     1381 cgaactgccc cagcttggct ggtaattgaa gcctttgatt gtcgcccagt ccatctttgt
     1441 ctcccccgaa tgggtctgca gcacttcttc ggcgaagccc cgccgcctca cacctgcttc
     1501 cgcgccttcc tgatgatatt cccgatggac gcggcatgca cctcggggca ccgggggaag
     1561 taccctcccg ccagcaccat gacaaaggga atgccacgtt tccgcgtttc gctccacacg
     1621 aacgcgtctc tctggatgat ccccgcgctc gtcagctccg tcgcggcgag cggatcatcg
     1681 gcgtgcacgt cgctcccggc gacaaagaaa acgatgtccg gcctgtgggt atccagcacg
     1741 tccggcacat gctcccgtaa aagcgccatg taggcctcgt cacctgttcc cgccgggaac
     1801 ggaacatcgc gcgaactggt cgccttttca aacgggaata tgtcctcctg gtgaaacgaa
     1861 agcgtaaaca cggagggatc tccggcgaaa atggtcgcgt tgccgtcgcc ctggtggacg
     1921 tcgcaatcca caatcgccgc gcgttgtagc agcttttcgc gcatgagaac tcggatggca
     1981 atcgccacat ccgccacgaa gcagaatccg gacccgtggt cggcgtgggc gtggtggaac
     2041 ccgccgccga tgttgaacac agtcccgcac ttcagcgcat cccgcgccgc ccgaaccgtg
     2101 ccgcccgcca ttgcgaaata gggcgggaga tagcgttccc ggatcgtgtc gggcgataca
     2161 ttccgcaggt aatcaacctc gaaaatctcc gccatggtct cgggatcggt ccagagacgc
     2221 atgtagtcgg gggtatgcac cagagcggcc tcctgttccg tcaggaggtc cggctcgatg
     2281 atcgccccgc cgtccagcag cccgtcggca tgcagcagat cgactatttt catgtacttg
     2341 cgcacgtcaa acggatggac cgattcctgc cccgacatgt cgagggcgta tccggggctg
     2401 tacacgacgc cccatggagc gtacaaaaca ctcatcattc aatcccgatc aaaaacgatt
     2461 caccgtacca tccgcggaag cggcgcgggt ttgcggaggc gttccctcgc gaaaactccc
     2521 ctccatcatc agtttccgcc tgtggcgccg gtccggaggc cttgttcaaa gatacgacga
     2581 agccccgttt tctcgtacga acattgaccc gtcgcgccct gtttcacact tttcacggta
     2641 aggcaatgcc gctacgcatc tttcaccaga aacgttcgct ctgtgagcgg gttctcttcc
     2701 gcattgcggc gctcctcctt gcgttcgtgc cgaacatgga gggtccgatg aatacacaag
     2761 ccccgattcg aacggctgcc gtagccggtc agttctaccc ggcatccccg gatgttcttc
     2821 gcgaagaggt tgacgccctt ctggcggcag cgccaaaaga tgaccgcatc accggcgaca
     2881 tcgtggcgat tgtggcgccg cacgccggct acatctacag cgggcggatc gccgcgatcg
     2941 cgtaccggca ggttcagggc agagaatacg acgcggtcgt gattctggca ccgtcccacc
     3001 gtgaagcatt tgccggcgtc tccctcatgc cggcgggggg atacgagacc cccctcggga
     3061 tcgcggggat tcatgagggc atcgcccgaa aactgctgga gcagggcggc tgcatccacg
     3121 aaacacggaa aggacacggc cccgagcatg cggtggaagt gcagattccg ttcatccagc
     3181 gcgcgttggg aagtgtgccg atcgtccccc tggtgatggg cgagcattcg tggccggtat
     3241 gcaactccgt ggcacgtgcg atcgccagag cagtcgaggg catgaacgtg cttgtcgtgg
     3301 cgtccagcga cctctaccac ggcgactcgg aagcggagtg cgaaaccatt gaccgcgtga
     3361 cactggcagc cgcggaatcc cgtcctccgc agcagttctg cgccgatctg gatgcggggc
     3421 gggcgcaggc ctgtggtggc gccccgatcg cggtggcgaa ggagtacgcc cgcctccttg
     3481 gtgcttccgg tgtccgcgtg ctggcgcatg ccacgtccgc cgacgtcacc ggccaccgcg
     3541 gcggctacgt ggtgggctac ggcgcaattg cgtacttccg aaacggggcc gcggtggagc
     3601 agtcctccct cagcgaggaa gaccgcgctc tgctgctgag acttgcgcgg gaatccgttc
     3661 gcgcggcggc tgccggccgg ccgttgcctc agccgccgaa aggtcacccc gcactggaag
     3721 aaccgaaagg cgccttcgtt acgctgcaca aagacggcga ccttcgcggt tgtatcggac
     3781 tgctgcaggc atatcagccc ctcgccgcca ctgtcatcga aatggcccag gccgcggcaa
     3841 tcaacgaccc gcgcttcccc cctctgcggg agcatgaact cgacacgacg gcgctggaaa
     3901 tctccattct ttcgccattt gaggtaacga ggccggaaca tgtcgaggtc gggaaacacg
     3961 gcatattcat taccggtcgc gggcggcgcg gcgttttgct gccgcaggtg gccaccgaac
     4021 aggggtggga ccgcgaaacc ttcctcgaac acgtgtgcct gaaagcgggg cttccgcgca
     4081 acgcatggca gagcagcgac gtggtgctgg aacgattttc ggcggagatc attcacgaca
     4141 cgggacccat cggccgcgaa gaaacccggc ggaattaggt gtctgcgatg ccgatcacca
     4201 ttctgctcgt ggaagacgag cccgggatcc gcagcgtgac ggcgaagtgg ctcgcccacc
     4261 acgggtacgc ggtcgacgag gcggaaaacg ggagagctgg gtgggagcgc gtgcaccaga
     4321 gcccgcccga cctcgtcatc accgacatcg ccatgccgga gatggacggc gtggagctct
     4381 gccggttgat caaaacgacg gaatccactg cggatatccc ggtgctcatg ttcacgagcc
     4441 atgcggaagc ggacctccag cttgccggca ccaaagcggg tgcggacgca tacattccca
     4501 aaaatggcga cctccgtgtg ttgaacgcgc gcattgaagc gctcgcggcc gcgcggtccc
     4561 gcgcccggca ggagaccgaa cggacgcgcc ggctcaccct cagccagagc gttaccaccc
     4621 tcgcgcacca catcaacaac agcgtgatcg ccatccacgc cactgcctcc gtggttgatc
     4681 cgaacaaccc gcaacaggct cagaagcttc ggcacgtctg ccagacggaa gcgcggaaga
     4741 tgctcctcgt gctgaaagcg ctgaaagaga tggccgaacg cgaggagctg aaaaccaccg
     4801 tctacgtcgg caacgaactg atgttcgacc tcgaagccgc cctcaggcag cttgattccg
     4861 gggccaaatc cccgccgcgc tgaccggagg tctcccttcc ccccgcttgc gctgtaatag
     4921 gtcgtgtagg acgtatagtc cgctctccca ctccgtccct ctttcttact gaggcaagga
     4981 ggtgtcttcg tgtcaaggct tttcgcgaag aagttgcaga tttgcatcag gatttttttg
     5041 gaaggttaag ccgaggacgg tctccgagtg gcgggaggac gaagtccgag cggcgtatcg
     5101 aggagcccgg cccggcggtt caccttccgt cggctcgtat gtaccgtgcc gatgcgtctc
     5161 gatacatctc cctcgtgagc cactcgacga ccggcaagtg ggcgcccgaa cagcggtcat
     5221 cgagtggcgt ccgccggacg gtcggcggac ggtgtatcga gatgaccgcg aaagccgcgc
     5281 ccggccaccc ggtctggatt cccgcctccg cgggaatgac ggattgtggg cgtgttcctg
     5341 accctctggc aaagaaaaag tcaatgactt tccgttcgtc atttccg
//
LOCUS       DAOTNG010000217         5379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12802_length_5379_cov_5.975019, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000217 DAOTNG010000000
VERSION     DAOTNG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_5379_cov_5.975019"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="P5179_10320"
     CDS             <1..1485
                     /locus_tag="P5179_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS95655.1"
                     /translation="GRCAMLRSRVVLFLLLALATSGLVSAARATDVSGIITSTIGVPI
                     DIAPGDTITHWTKAGSPYHVKGTVTIPAYNTLSIEPGVDVLFDADVQFVVQGSLYARG
                     TGADSIRFLKGTAGSWRGIRISGGWTSTLEYVRISGGYARGSEPDCRGGGLYVSGVGT
                     WVDIHHSAIVGNRAAQDGGGIANEAGEIGGVYMTHCRVSDNSSEGEWGVGGGISSSGW
                     MQMYYCVISGNATSGTGSGGAVSNVGAAPFFGFVMYGCVICGNAAGNAGGAIDNAGNG
                     SLTLYECTISGNTAPRASAINNSDSASAELLNTIVWANTPAEQIVSNGGTVTATYSDI
                     QAGFEGTGNISADPLFVDAANGDYSLRPDSPCIDTGSPSTPLDPDGTRTDISAIPYNH
                     PVGVANNPLPAAFTLAQNAPNPFNPSTTIRFTLPEAGHVTLAVYDINGRLVRTLVGQA
                     FLPGHHEVVWDGKDAMGRAVASGVYVYRLTAPQGVVTKRMTLLR"
     gene            complement(1641..3056)
                     /gene="lpdA"
                     /locus_tag="P5179_10325"
     CDS             complement(1641..3056)
                     /gene="lpdA"
                     /locus_tag="P5179_10325"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRS95656.1"
                     /translation="MAQTQVAVIGGGPGGYVAAFHAADLGLKVTLIDTDVNPGGVCLY
                     RGCIPSKALLHVAALINEADHAAAWGVTFARPTVELDKLRAWKQSVVQKLTGGLGELV
                     KRRKITHIQGRARFAGPTTLRVDKTSGGTEEVTFEYAILATGSRPTSIPTLSIPSPRV
                     LDSTGALELADVPESLLVVGGGYIGLELGTVYQALGSRVSVVEMTPGLLPGADRDLVG
                     PLARRIEKKFEKVMLETTVTSLAEEGDGIRARFKDRTGAESDALFSKVLIAVGRRPNS
                     ADLGLETTKVVVNARGFVEVDAQRRTAEPTIFAIGDVAGEPMLAHKASHEGMVAADVI
                     AGGKSVFEPYAIPAVVFTDPEIAWCGLTETQAAKEGREIKVARFPWAASGRATTLDRN
                     DGVTKVIVDPKTERVLGVGMTGAGAGELIAEGTLAVEMSALASDLKLTIHPHPTLSET
                     LMEAADVYFGQSPHLWAPKRG"
     gene            complement(3088..4476)
                     /locus_tag="P5179_10330"
     CDS             complement(3088..4476)
                     /locus_tag="P5179_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95657.1"
                     /translation="MSKPNILIIMPDQQRADGMGCAGRFPIRTPNMDRLAEEGTRFAQ
                     AATVCPVCMPARASFINGLYPHNHGMWVNHGEMPAEDETFFHHLQRAGYYTAHVGKSH
                     YHEHRGGEHLREREPYMHARGLDYVHETTGPYATCRTASYLTDSWAGTNYWELFKQDY
                     LERAKLGGAMVRPSPLPVDAFPDSYVGKQAVKFVNEYGGKQPFCMFVGFGGPHDPWDA
                     PGEYATMYDPASIPPPIPHSTQVDQFPGWMRNLVDFRTFGPEVQAQAAAIRANYFGKI
                     SLIDHWVGEILNAYASRGWLDDLLVVFWSDHGEMAGDHGRIQKCTFHESSLRVPLIIR
                     WPGRVPANTVSNALAEIIDVFPTLLETVGCEPSRRNQGKSLWPVLRGEQEDLREFQLS
                     EIDWQGRKIMIRSRRYKYAVDELGRGYMLYDLEKDPDEQNNLIADDAHRPLEACLREV
                     LLARLVGAQYTM"
     gene            complement(4520..>5379)
                     /locus_tag="P5179_10335"
     CDS             complement(4520..>5379)
                     /locus_tag="P5179_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRS95658.1"
                     /translation="EIGIDITKVPGTGEGGRITHDDVKAFSRAQNEARVVATAGSGIP
                     AAALPDFEKWGALERQEMSNVRRKTAEHMAFCWATIPHVTQFDTADITALEDWRKRAA
                     KQVEAAGGKLTVTAVVLKIVGAALKAFPQFNASIDVASNQIVLKKYVHVGIAVATDRG
                     LLVPVIRDVDKKSITELSVELSEVSEKARSRKLGIEDMQGGCFTISNLGGIGGTYFTP
                     IVNWPEVAILGLSRAQVQPVYVDGELKPRLIMPLSLSYDHRVVDGVDGAAFVRWIARA
                     LEDPLTILL"
BASE COUNT          934 a         1827 c         1571 g         1047 t
ORIGIN      
        1 gggaggtgcg ccatgctgcg gtctcgcgtt gttcttttcc tcctgctcgc cctcgccaca
       61 agcggccttg tcagcgccgc ccgggccacc gatgtgtccg gcattataac cagcacgatc
      121 ggtgttccaa tcgacatagc gcccggcgac accatcactc attggacgaa agcaggttcc
      181 ccttaccacg tgaagggcac ggtcacgatc cctgcctaca acaccctctc catcgaaccc
      241 ggcgtggacg ttctcttcga cgcggatgtg cagtttgtgg tgcagggcag cttgtatgcc
      301 cgcggcacgg gggccgactc catccggttt ctaaagggaa ccgctggatc atggcggggt
      361 atccgcattt ccggcggctg gacaagcacc ctcgaatatg tccgtatcag cggcgggtat
      421 gcacggggca gcgagcccga ctgccggggc ggcggtttgt atgtctccgg cgtcgggacg
      481 tgggttgata tccatcactc agcgatcgtt ggcaaccggg cggcgcagga cggcggaggc
      541 atcgcgaatg aagcgggcga aatcggcggc gtctacatga cgcattgccg ggtttcggat
      601 aactcgtcag agggtgaatg gggagtcggc ggtggcattt ccagctctgg ctggatgcaa
      661 atgtattatt gcgtcatctc cggcaacgcc acctccggta ccggatcagg tggcgccgtg
      721 tccaacgtcg gcgcagcccc attttttggt tttgtcatgt atggttgtgt catttgcgga
      781 aatgccgctg gtaatgcggg gggagcgatt gacaatgctg gcaatgggtc gttgacgtta
      841 tacgaatgca cgatttccgg caacactgcg cctcgtgcaa gtgcaatcaa caatagtgat
      901 tccgcttcgg cagagcttct caacacgatc gtttgggcaa acaccccggc agagcagatc
      961 gtctcaaacg gtgggacggt caccgcaacc tattcggata tacaggccgg gttcgagggc
     1021 acgggcaaca tcagcgcaga cccgctgttc gtggatgcgg ccaacggcga ttactcgctt
     1081 cgcccggatt ccccgtgcat cgataccggc tcgccatcga cgcctttgga cccggatggc
     1141 acgcgtacgg acatcagcgc catcccgtac aaccacccgg tcggggttgc gaacaatcct
     1201 ctcccggcgg cgttcacgct tgcgcagaac gccccaaatc cgttcaaccc gtccaccaca
     1261 atacggttca cgctgccgga ggcggggcac gtgacgctgg cggtgtatga catcaatggc
     1321 cgtctcgtgc gcacgttggt gggccaggca ttcctgcctg gccaccacga ggtcgtgtgg
     1381 gacgggaagg acgctatggg tcgcgcggtt gccagcgggg tgtacgtcta ccgcctcacc
     1441 gcgccgcagg gcgtggtgac gaagaggatg acactattgc gttagagtgg cggtcactga
     1501 gcctgcgcac agcgcaggag gcttgtcgaa gtgccgccgc gatgtgtcgg agccccgcgc
     1561 ggcgtttcga caggctcaac gaccgcgcgt cagggcgcag tctggtcagg taacagcgtt
     1621 cgcacgcagg ggcgacgcac ctacccccgc ttcggcgccc agaggtgcgg gctctgaccg
     1681 aaatacacgt ccgccgcttc cataagggtc tccgagagcg tcggatgcgg gtggatggta
     1741 agcttcaggt cgctggcaag cgccgacatc tccacggcga gcgttccctc ggcaatcaat
     1801 tcccccgcgc ccgcccccgt catacctaca cccagaacac gctcggtttt cgggtccacg
     1861 atgaccttcg ttacgccgtc attgcggtcc agtgtcgtcg ccctgcccga cgccgcccag
     1921 gggaaccgcg ccactttgat ctcgcgccct tctttcgccg cctgcgtctc cgtcagcccg
     1981 caccaggcga tttccggatc agtgaacacc acggccggga tggcataggg ctcgaacacg
     2041 gacttcccgc cggcaatgac gtccgccgca accatcccct catgcgaggc cttgtgcgcc
     2101 agcatcggtt ccccggccac gtcgccgatg gcaaagatcg tcggctccgc cgtgcgccgt
     2161 tgagcgtcca cctcgacaaa tccgcgcgcg ttcaccacga ccttcgtcgt ttcgagacca
     2221 aggtccgcgc tgtttggcct ccgccccacg gcgatgagca ccttgctgaa cagggcatcg
     2281 ctctcggcgc cggttctgtc cttgaaccgc gctcgaatgc cgtctccctc ttccgcaagt
     2341 gacgtaacgg tggtctccag catcaccttt tcgaacttct tctcaatacg ccgcgccaac
     2401 ggaccgacga ggtcgcggtc ggcccccggc agcagccccg gcgtcatttc gaccacgctg
     2461 acccgcgaac cgagcgcctg gtacaccgtt ccgagttcca gcccgatgta gccgccaccc
     2521 accacgagca ggctttccgg cacatcggca agctccaacg cgccggtgga atccagcacc
     2581 cgcgggctcg ggatggaaag ggtggggatg ctggtgggcc gcgatccggt agcaagaatg
     2641 gcgtactcga acgtcacctc ctccgttcca ccggacgttt tgtccacgcg aagcgttgtc
     2701 gggccggcaa accgggcgcg cccctgaatg tgagtgatct tgcggcgttt gaccagctcc
     2761 ccaaggccgc ccgtcagctt ctgcaccacg ctctgtttcc aggcgcgcag cttatcaagc
     2821 tccacggtcg gcctggcaaa cgtcacaccc catgcagccg catgatccgc ctcgttgatg
     2881 agcgcggcaa cgtggagcaa cgccttggaa gggatgcagc cccggtacag gcacaccccg
     2941 ccggggttca catccgtgtc gatcagtgtc accttcaggc caagatcggc tgcgtggaac
     3001 gccgcgacgt acccgccggg ccctcctccg atcacggcaa cctgtgtctg cgccatgggt
     3061 ttttcctttt ctcgccactc gtcgcgtcta catggtatac tgcgccccga caagccgcgc
     3121 cagaagcacc tcgcgcaggc aggcttcgag cggcctgtgg gcgtcgtccg caatcaggtt
     3181 gttctgctcg tcggggtcct tctccagatc atacagcatg tacccccgcc cgagttcatc
     3241 caccgcgtat ttgtacctgc gggagcgtat catgattttt ctgccctgcc agtcaatctc
     3301 gctcaactgg aactctcgca ggtcttcctg ttcccccctg agcaccggcc agaggctctt
     3361 cccctgattt cgccgcgagg gctcgcaccc caccgtctcc agcagcgtcg gaaacacgtc
     3421 tatgatctcc gcaagagcgt tcgagacagt gtttgcgggc acccttcccg gccagcggat
     3481 gatgagcggc acccgcagac tggattcatg gaacgtgcac ttctggatgc ggccatggtc
     3541 acccgccatc tcgccgtggt ccgaccagaa taccaccaga aggtcatcca gccagccccg
     3601 cgaggcatac gcgttcagta tctcgccgac ccagtggtct atgagcgaga ttttcccgaa
     3661 atagttcgcg cggatggctg ccgcctgcgc ctgcacctcc gggccgaacg tccggaagtc
     3721 caccaaattg cgcatccagc cggggaactg atccacctgc gtggagtgcg gaatcggcgg
     3781 cgggatgctg gccgggtcgt acatcgtggc gtactcaccc ggggcgtccc acgggtcgtg
     3841 cggcccgccg aagcctacaa acatgcagaa tggctgcttg ccgccgtact cgttcacgaa
     3901 cttcaccgcc tgtttgccga cgtagctgtc cggaaacgcg tccaccggca gcgggctcgg
     3961 gcgcaccatc gcgccgccaa gcttcgcccg ctcgaggtaa tcctgcttga acagctccca
     4021 gtaattcgtt ccggcccagc tatccgtcag gtacgacgcc gtccggcacg tggcgtaggg
     4081 tccggtggtc tcgtgcacat aatccagccc gcgggcatgc atatagggct cgcgttcccg
     4141 caggtgctcg ccgccgcggt gttcgtggta gtgggatttg ccaacatgcg cggtgtagta
     4201 cccggcccgc tgcaggtgat ggaaaaacgt ctcatcctcc gccggcattt ccccgtggtt
     4261 gacccacatg ccgtggttgt gcggatacag gccgttgatg aacgacgccc gcgccggcat
     4321 gcacaccggg cacacggtgg cggcctgcgc aaatcgcgtc ccctcctcgg ccagccggtc
     4381 catgttcggc gtgcgaatcg gaaaacgccc cgcgcatccc attccatccg ctctctgctg
     4441 atccggcatg atgatcagga tgttcggttt cgacatctgt cccctcccat cagggttgga
     4501 gcgtgaaccg gtatcggacc tacaaaagaa tcgtcagcgg atcctccagt gcccgggcaa
     4561 tccatcgcac gaacgccgcg ccgtcaactc cgtccaccac gcgatggtcg tacgagagtg
     4621 aaagcggcat gatgagcctc ggtttcagct cgccgtccac gtacaccggt tgcacctgtg
     4681 cgcgtgagag acccagaatg gcaacttccg gccagttcac gatgggcgtg aaatacgttc
     4741 ccccgatgcc accgaggttc gaaatggtga aacatccgcc ctgcatgtcc tcaatgccga
     4801 gcttccggct cctcgccttc tccgatacct cgctcagctc caccgaaagc tccgtgatgc
     4861 tcttcttatc cacatccctg atgacaggca ccagcagccc ccggtcggtg gctaccgcga
     4921 tgccgacgtg cacgtacttt ttcagcacga tctggttact ggcaacgtca atactggcat
     4981 tgaactgcgg gaatgccttc aaggccgcgc ccacgatctt cagcaccacc gccgtcaccg
     5041 tgagtttccc gcccgccgcc tccacctgtt ttgccgcgcg cttgcgccag tcctccagcg
     5101 cggtgatatc ggccgtgtcg aactgcgtca catggggaat cgtcgcccag cagaacgcca
     5161 tgtgttcggc ggtcttccgg cgcacgttgg acatctcctg ccgctccagg gcgccccatt
     5221 tctcaaaatc aggcagcgcc gccgccggaa ttccgcttcc cgcagtcgcc accaccctgg
     5281 cctcgttctg cgcgcgcgaa aacgccttca cgtcgtcgtg ggtaatgcgg ccgccctcac
     5341 ccgttcccgg caccttggtg atgtctatcc cgatttccc
//
LOCUS       DAOTNG010000218         5378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12804_length_5378_cov_4.320872, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000218 DAOTNG010000000
VERSION     DAOTNG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_5378_cov_4.320872"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..176
                     /locus_tag="P5179_10340"
     CDS             72..176
                     /locus_tag="P5179_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRS95659.1"
                     /translation="MWGPTGTAASLMKGMKQAFDPSGVLNPGRFVEGI"
     gene            195..1505
                     /locus_tag="P5179_10345"
     CDS             195..1505
                     /locus_tag="P5179_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRS95660.1"
                     /translation="MFAKRKRLLPDAVKEQENNLLACVHCGFCLPHCPTYSVLGDEND
                     SPRGRLYLMRAVAEGRIKPDDAYTRHIESCIVCRACETACPSGVRYGTIMEAARCDLR
                     RLGASDMGWLARVLRWMGLYLLPRRSLLRPAVFPMRIIRHLRPTDGWWRWLPSIVRLG
                     LEMLPPPPLKRKFAPVSSHAPDKGVVVQFRGCVMDELFKNVNQATTRVLEVNGFRVTV
                     PQAQTCCGSLHDHSGFVDAARELARKNIDSLEDGNDTPVVVNAAGCAALMKEYGELLK
                     DDPRYAARAHRFASRVCDVTELLARTGVVKGASIQRKVTYDAPCHLYHAQKVKTAPLD
                     VLKAVPDLDFRPLRGADRCCASGGIYNLTHPDIAEEVLQRKLSALFETGAETLVTANP
                     GCQMQIQQGVRTAGMDVSVAHVIELLDESYEKAGYYDSTTSLKG"
     gene            1508..2689
                     /locus_tag="P5179_10350"
     CDS             1508..2689
                     /locus_tag="P5179_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRS95661.1"
                     /translation="MLITDVDAFVLAMPHVTDAADGTQDTFLVRIRTNTGVTGWGESD
                     ASPLVSLAAYYCPMSHGNIINIRESLLGERVDTPEDIRRLHQKVRRQGLDIEQIDHAY
                     SAADIALWDALGKQLDEPVYHLLGSPIAYPKLPYASVLFADTPQETLERARGLRKLGF
                     RAAKFGWGPMGKRGADFDVDLVAAAREGMGEEAEIMIDAGVAWGTDYETAYARALAFS
                     PFRPTWLEEPLLPDAVDEYGKLKALNPPVPIAAGEGAARFRTAQDILCNGKVDFLQID
                     VGRIGGLTPAREVAVLTRNRGAIYVNHTFKSHLSLAASFHVFAGESMFRYLEYPAAAS
                     DLAKRLVAAPLVPGSDGLVRLPRATGLGIELDMTTAKEYLQPVRIELGGKVLVEINDL
                     A"
     gene            2698..>2937
                     /locus_tag="P5179_10355"
     CDS             2698..>2937
                     /locus_tag="P5179_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015741246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS95662.1"
                     /translation="MGTLDGKNAVVTGAGSGIGRSIAVRFAREGASVALLGRRKEMLE
                     ETLRAMPKGDHLVIPTDVTAAVQVAHAKDLIRDRWG"
     assembly_gap    2938..3037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3038..3617
                     /locus_tag="P5179_10360"
     CDS             <3038..3617
                     /locus_tag="P5179_10360"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS95663.1"
                     /translation="TAAVQVAHAKDLIRDRWGALHVLVNNAGVSVAAHPIASELAEWM
                     LPIDTMLMGAVHCTRAFVPLMPVGSRIVMVTSIHRERVEKGASAYATAKGALTQYVRA
                     LAVELADQGILVNAVAPGFVDTPMSVKAGGKNELAGEWFRTNYVEGHHLPLRRAGKPD
                     EIAGAVLFLAGADATYITGETITVDGGLTITF"
     gene            3634..4656
                     /locus_tag="P5179_10365"
     CDS             3634..4656
                     /locus_tag="P5179_10365"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS95664.1"
                     /translation="MSKARIKGILTPNLVPLDRNGNIAEDELRRYVEWLVQKGVSGLY
                     PNGSTGEFTRFSPEERREIIRIVASQVNGRVPVLAGAAEATVRDTLAACAAYAEMGCT
                     AAAICAPYYFKLSQDSVGEYFAELARHTELDIVLYNIPQFANEISLDTIERLADFPRI
                     VGIKDSSRDFPRFLNLINRMRPRRPDFSILIGTEEIILPALLMGGDGGTIATSGIVPE
                     VVMKLYALAESGKIEEARKIQFKLLELINVMLFGAGFPEGFRAAASLRGFVMGQSRQP
                     LSPKEKCDLNAVRQTLHCILSEHGFVAEIEGAVGCEPLGQDASPSETEIQAIVAEVVR
                     QMRTNR"
     gene            4660..5289
                     /locus_tag="P5179_10370"
     CDS             4660..5289
                     /locus_tag="P5179_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRS95665.1"
                     /translation="MSDTHEALTVVEVQGLPPAVVVADAMVKSAAITLIALEVNTLGA
                     LTIKASGDTAAAAAAHDAGKAIARQLDALVGATVIPRYSSPAYPMWIACKPSVSGLLK
                     SRNHLIPADGLLPVDDRAMGMLETRGFAGAVAALDAMLKSADVELLAKEKIGQTRVSV
                     LVRGDVSAVTASLAAGAVQAERVGTLASAHVIPHPDPVIQALFPRPQGQ"
BASE COUNT         1130 a         1529 c         1584 g         1035 t
ORIGIN      
        1 gccaggtggt gtcgtcgggc ggttcggtca tcctgcagaa gttgccaccc ggatggaaag
       61 agcacatcga cgtgtggggg ccgaccggta cggccgcgtc cctgatgaag ggaatgaaac
      121 aagcctttga tccttccggg gtgctcaatc ccggcaggtt cgtggaaggt atctgaagca
      181 gtctggaagt aaccgtgttt gcaaaacgaa aacgcctgct gccggatgca gtaaaagaac
      241 aggagaacaa tctcctcgcg tgcgttcatt gcggcttctg tctcccccat tgtcccacgt
      301 acagcgtcct cggagacgaa aacgattctc ctcgcggaag gctctacctc atgcgggccg
      361 tggccgaggg cagaatcaaa cccgatgatg catacacccg ccacatcgaa tcctgcatcg
      421 tctgccgcgc gtgcgagaca gcgtgccctt ccggcgtgcg ctacggaacc ataatggagg
      481 cagcgcggtg tgatctgcgt cggctgggcg catcggatat ggggtggctt gcgcgggtat
      541 tgaggtggat gggtctttac ctcctgcccc gccgttctct gctcagaccg gcggtattcc
      601 cgatgcgtat catccggcat ctgcgaccga ccgacggctg gtggcggtgg ttgccatcaa
      661 tcgtccggct ggggctcgaa atgctgcctc cgccgccgct gaaacggaaa ttcgcgcccg
      721 tttcatcgca tgcccctgac aaaggggttg tcgtgcagtt tcgcggatgc gtgatggatg
      781 agctgttcaa aaacgtgaat caggctacca cacgcgtcct tgaggtgaac ggttttcggg
      841 tcacggttcc tcaggcacag acctgttgcg gttcgctcca tgaccacagc ggatttgtag
      901 acgctgccag ggaattggcc aggaaaaaca tcgattcttt ggaggatggc aacgacactc
      961 ccgtcgtcgt caacgcggca ggctgcgcgg ctttgatgaa ggaatacggg gaattgttga
     1021 aagacgaccc ccgttacgct gcgcgtgccc atagatttgc atctcgcgta tgcgatgtga
     1081 ctgagttgct cgcccgaacc ggggttgtca agggcgcatc tatccagcga aaagtcacat
     1141 acgacgcacc atgccacctg taccacgccc agaaagtgaa aactgcccca ctcgacgttc
     1201 tcaaagcggt tccagacctc gatttcagac cgttgcgcgg agccgatcga tgttgtgcgt
     1261 ccggaggcat atacaacctc acgcatcccg acatagccga agaagtgttg cagcgcaaat
     1321 tgagcgcgtt gttcgaaacg ggcgcggaaa ccttggtgac ggctaatcct ggatgtcaga
     1381 tgcagattca gcaaggcgtc cgtacggcag gtatggacgt ttccgttgct cacgtaatcg
     1441 aacttctgga cgagtcgtac gagaaagccg gttattacga cagtacgacc agtttgaaag
     1501 gttaacgatg ctgattacgg atgttgatgc gtttgtcctc gcaatgcccc atgttaccga
     1561 cgcggctgac ggcacgcagg atactttcct ggtgcgcatt cgaacaaaca ccggggtgac
     1621 gggttgggga gaatccgacg cgtcgccgtt ggtgagcctt gccgcatact actgcccgat
     1681 gtcacacgga aatatcatca acatcaggga atccttgctc ggggagcgtg tcgacacgcc
     1741 ggaagatatc agaaggctgc atcagaaggt ccgcaggcag ggactggata tcgaacagat
     1801 cgatcacgcg tacagcgctg ccgatatcgc cctgtgggac gcgctgggaa agcaactgga
     1861 tgagccggtg tatcacctcc tcgggagtcc aatcgcgtat ccaaagctcc cctacgcgtc
     1921 tgttttgttc gcagatacgc cgcaggagac ccttgaacga gccagggggc tgagaaaact
     1981 gggcttccgt gccgcgaaat tcggttgggg accgatgggg aaacgcggag cggatttcga
     2041 cgtagacctg gttgccgccg cgcgcgaggg tatgggtgaa gaagcggaaa tcatgatcga
     2101 cgcaggagtc gcctggggaa ctgactatga aaccgcatat gccagagctc tcgcgttctc
     2161 accattccgg cctacctggc tcgaggagcc tctcctgcca gacgcggtag acgaatacgg
     2221 caagttgaaa gcgctgaatc cgccggtgcc catagctgcg ggagaaggcg cagcacgctt
     2281 cagaacggct caggatatcc tttgcaacgg aaaagtggac ttcctccaga ttgacgtggg
     2341 acgcatcggc ggattgactc cggcaaggga ggtcgcggta cttaccagaa atcgcggtgc
     2401 catctacgta aaccatacct tcaaatccca tctttcgctc gcagcatcgt tccatgtttt
     2461 cgcaggcgag tccatgttca ggtacctcga gtaccctgct gcagcatcag accttgcaaa
     2521 gcgcctcgtt gccgcgccgt tggttccggg ttcggacggg ctggttcgtc tgccgcgagc
     2581 cacgggactc ggaattgaac tggatatgac gactgcgaag gaatacctgc aaccggtgcg
     2641 gatcgaactg ggcgggaaag tgttggtcga aatcaacgat ttagcttgag gaacgagatg
     2701 ggaacgttgg acggcaaaaa cgccgtggtt acgggggcgg gaagcgggat tggacgatcc
     2761 atcgccgtgc gttttgcacg cgaaggagcg tctgtcgcgc tactggggcg acggaaagaa
     2821 atgctcgagg aaaccctccg tgccatgccg aaaggcgacc atcttgtgat tccgaccgac
     2881 gtaactgccg cagttcaggt agcgcatgca aaagacctga tccgtgatcg ctggggtnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac tgccgcagtt caggtagcgc
     3061 atgcaaaaga cctgatccgt gatcgctggg gtgctctcca cgtgctggtg aacaacgcag
     3121 gcgtatcagt ggcagcccat cccatagcat ctgaactcgc ggaatggatg ctccccatcg
     3181 ataccatgct catgggagct gtgcactgca cgcgcgcgtt cgtccccctg atgcctgtcg
     3241 gaagcaggat cgtgatggtt acaagtatcc atcgcgaacg ggtggagaaa ggcgcctccg
     3301 cctacgcaac agccaaaggc gcgcttaccc agtacgttcg ggcccttgcc gtagaacttg
     3361 cggaccaggg catcctcgta aatgcagttg cacccggctt tgtggatacc ccgatgtccg
     3421 tgaaagccgg cgggaaaaac gagctcgccg gggaatggtt tcgcaccaac tacgtcgagg
     3481 gacaccatct ccctctccgc cgtgctggca aaccggacga aatcgctggc gcggtgctat
     3541 tcctcgcggg cgccgacgcg acctacatta ctggagaaac aatcaccgtc gacggcggat
     3601 tgaccatcac cttctgaaga agagtggact gccatgtcaa aggccagaat caagggaatc
     3661 ctcacgccga atctcgtgcc gctggatcgt aacgggaata tcgcggaaga cgaactgcgt
     3721 cgctacgtgg aatggctcgt tcagaagggc gtgagcggcc tctaccccaa cgggagcacc
     3781 ggagaattca cccgtttcag tccggaagaa cggcgcgaaa tcatccggat tgtcgccagc
     3841 caggtgaatg gccgggttcc cgtcctcgcc ggcgcagccg aagcaaccgt ccgtgataca
     3901 ctggctgcct gcgccgcgta tgccgaaatg ggttgcactg cggcagccat ttgcgcgcct
     3961 tactatttca aactctccca ggattccgtg ggcgaatact tcgccgaact cgcgcgccac
     4021 acggaactcg acatcgtgct ttacaacatc cctcagtttg ccaatgaaat cagcctggac
     4081 accatcgaac gccttgctga tttcccccgc atcgtcggga tcaaagactc cagccgggat
     4141 tttccgcggt tccttaacct gatcaaccgt atgcggccgc gccgccccga tttcagcatt
     4201 ctcatcggta ccgaagagat catactgccc gcgttgctga tgggtgggga tgggggaaca
     4261 atcgccacaa gcggaatcgt tcccgaagtt gtgatgaagt tatacgcgct tgcagaatcg
     4321 ggcaagatcg aggaggcgcg caaaatccag ttcaagctgc tggagctcat caatgtcatg
     4381 ctgttcgggg cgggattccc tgaaggcttc cgtgcggccg ccagcttgcg gggcttcgtc
     4441 atggggcagt cccgccagcc actctccccg aaagaaaaat gcgatctcaa cgcggtgcgc
     4501 cagacccttc actgcatcct cagcgaacat ggcttcgtcg cggaaatcga aggagccgtt
     4561 gggtgcgaac cccttggaca ggacgcctcc ccttccgaaa cagaaattca ggccattgtc
     4621 gcggaggtcg tgcgtcagat gcggacgaac cggtaacaca tgagcgacac gcacgaagct
     4681 ctgaccgtcg tggaagttca gggccttccg cccgcagtgg tcgtggcaga cgcgatggtg
     4741 aagtcggcgg cgatcactct catcgcgctc gaggtcaaca cgcttggtgc cttgaccatc
     4801 aaagcgtcgg gggatacggc agcggcagcg gccgcgcacg acgcgggaaa agcaatagcc
     4861 cggcagctgg atgctctggt cggcgcgaca gtaatcccgc gctactcgtc gcccgcatat
     4921 cccatgtgga tagcgtgcaa accctcggtc agcggcctcc tgaaatcccg aaaccacctg
     4981 atccccgcag atgggctcct tccggtggat gaccgtgcaa tggggatgct cgaaacgcgt
     5041 gggttcgccg gcgcggtggc ggcacttgat gcaatgctca aatccgcaga tgtggagctt
     5101 ctcgccaaag agaagatcgg gcagacaagg gtttccgttc tcgtcagggg cgacgtttcg
     5161 gctgtcacag cgtctctcgc tgccggggcc gtgcaggcag aacgagtcgg caccctggct
     5221 tcagcgcatg ttatcccgca tcccgaccct gtgattcaag cccttttccc tcgaccgcag
     5281 ggccagtaat cccttcttga agcagactac gggcgcccac ccagaccccc cgcgcgcctt
     5341 ccagcgagac gccgtccaca atggttccgg aagtgacg
//
LOCUS       DAOTNG010000219         5375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12810_length_5375_cov_5.055827, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000219 DAOTNG010000000
VERSION     DAOTNG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_5375_cov_5.055827"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="P5179_10375"
     CDS             <1..899
                     /locus_tag="P5179_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRS95666.1"
                     /translation="PDADRPPDDSRVRALLADATICITSWGSPNLGESVIENASALKL
                     VAHAAGSVKPIVSPALWKRGVRVTSAAAAIALGVAETTLGWIIIAAKRGILASQVTRA
                     GGWLKDMAFPPGDLPGKVIGIVGASHVGRNVIRLLKPFPVTVMVYDPYLSDDDATALG
                     VVKSELDDLMMRSDIVSLHAPKTEETHHMINARNLALLRDGATLINTARGALIDEAAF
                     VEHLRKGRTWAILDVTDPEPPPPDHPFRSLPNVVLTPHFAGTVESGRSRIGDCVLEEI
                     KRFIAGEPLHYEVHEEMLPRIG"
     gene            complement(906..2531)
                     /locus_tag="P5179_10380"
     CDS             complement(906..2531)
                     /locus_tag="P5179_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS95667.1"
                     /translation="MNNLRRAVQLIAFAVFLAGTETYWLEYDPLISIASLVSREFSWG
                     LVLRGLLLASATLLIGRFFCGWLCPLGTFLDAAHRCGVRPSKDATRENGTRARWKFVF
                     LAFLIVAAAFGTTTFVLFDPLVLFSRTRELVLIPAAAWVGTGVVRAVRLVPGQESFAF
                     RAVENPPAYGFLTLTAAVFAGILILEVIERRFWCRNLCPLGAFLNLLSRTGLVRRSVE
                     SSCSRCARCERDCPTGAIPVNDFTKTRRGECIQCLRCQRACSKGTVRFVFGGPPQIRG
                     FEPRRRQVLLAAGAGVAAGFLGDASTKASTRPGAHLRPPGAIPEEEFLSRCTRCLICV
                     DACPTHGLQPSGLEIGWEGLWTPKLVPASGGCEEPCNACTQICPTLAIRRLSHEEKSF
                     AKIGTARIVKERCVAWEELKACLVCDEVCPYDAIEFLVVSDHRGTQNRPVVHADKCMG
                     CGLCEHACPVREPRAIVVDNYGEERIGSGSYITPAKQAARKVGDDRATDYFREFRAGA
                     GVPADSVRGAGSTPAAGADTIFEEPLPPGFVNP"
     gene            complement(2560..3480)
                     /locus_tag="P5179_10385"
     CDS             complement(2560..3480)
                     /locus_tag="P5179_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRS95668.1"
                     /translation="MGLSRRDFLKAGAMAAGGLVLPKWAYSAVSRAATSPDVVHVTGG
                     TVELAVRTSIDRLGGIGAFVRRGQKVLIVPNAGFPNPAEMATTTDPETIRAIATLCKE
                     AGASSITVSSYPVREPDLCFERSGIADLARMNGVNVVPLTSGSAYVPLRIPDGIDARE
                     VEVATVVRQSDVLIAVPVAKSHSSAGVSFAMKGMMGLIRSRGPFHARYDLHQAIVDLS
                     KGIKAQLVVVDARRALVTRGPGGPGRVETPNAIVAGRNQTTVDAYTVSLARWYNRAFT
                     ADQVRHLRLAGEQGLGLTDLSAMNVVRVTL"
     gene            complement(3569..>5375)
                     /locus_tag="P5179_10390"
     CDS             complement(3569..>5375)
                     /locus_tag="P5179_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95669.1"
                     /translation="NTALRFTRMAFTQQELMLAYVTMLVPSAIPSDGLATRLVPLLVE
                     LYYYATPINEWAILHQPNVPHWMTPQGQEVIDWFFTGKPTGAAIPWEAWVKPLALWIL
                     LAAALCLIMISLSVAFRKRWMDAERLQFPLAQIPLMVMGDDPTPSWYSSFFRQPLVWA
                     GIAIPFFFHTVNGLNIYFPSIPPIGLANITLGSLFSGSRVFTDVPFYRWANIQFNFYW
                     SIVGISYLLRSEVSLSVWVFEWFYYIEHIIFESTSIGDGQFQWTPLHTFGYTLAARYQ
                     RLGAMVLAMLVYFIASRGEIREVLLAAFGQRKRLKKGSGAESSIPWWSLWAFLLGVGI
                     YFSWTAAAGIMFSVSFLLLFFFLVIAITTARIVAATGLLWVHDYFVPMHGMTVIVGSA
                     RVDPRTWTNMGFVDFSTLSNRANIMPQLLDGMKIARQTSIRHGHYFLGVGLGLVTAVF
                     VSFAMILWLAYTYGGANLEPWHFFNGGDWLFNRTAGFQRYHVYTHWTAISLMGAGAVF
                     MSVLLYLHRTFLWWPIYPLGFLIGDTVASNEIWFPVFLGWVIKAAIIRFSGASAYNRL
                     KNGALGLILGEFLSVGLWLVIDGFSGVTLHRVFPK"
BASE COUNT         1212 a         1639 c         1544 g          980 t
ORIGIN      
        1 aaccggacgc ggaccggcct cctgatgatt cccgtgttcg ggctcttctt gcggacgcca
       61 ccatctgtat cacatcctgg ggaagcccga atcttggcga gtccgtcatc gagaacgcaa
      121 gtgccctgaa gctggttgct catgcggccg gatcagtgaa gccgatcgtc tcgcccgctc
      181 tctggaaacg tggggttcgt gtcacgagcg ctgccgccgc gatcgcgctc ggtgttgcgg
      241 agaccaccct cggttggata atcatcgcgg ccaaacgcgg catcctcgcc agccaggtta
      301 cgagggcggg gggatggctg aaggatatgg cgttcccccc gggtgatctt cccggcaaag
      361 tcattggcat cgtcggcgcg agtcatgtcg gccggaacgt cattcggttg ctcaagccgt
      421 tccccgttac agtgatggtg tatgatcctt acctctctga tgacgacgcg acggcacttg
      481 gcgtggtcaa gtcagaactc gacgatctga tgatgcgctc ggatatcgtt tcgcttcacg
      541 ctccaaaaac cgaagaaaca catcatatga tcaatgcgcg taatctggcg cttttgcgtg
      601 acggcgcaac ccttatcaac acagctcgag gggcgctgat tgacgaagca gccttcgtcg
      661 agcacctgag gaaaggcaga acctgggcca tcctcgacgt gaccgatccc gaacctccgc
      721 cccccgatca cccgttccga tctctgccaa atgttgttct gacgcctcat ttcgcgggaa
      781 cggtagaaag tggccgttct cgtatcggag actgtgtgct cgaggaaatc aaacggttta
      841 ttgcaggcga gccactccat tacgaggtgc acgaggagat gcttccaagg atcgggtaga
      901 ggacctcaag ggtttacaaa ccccggcggc aacggctcct caaaaatggt gtctgcgccc
      961 gccgccggag tggaaccagc gccgcggacg ctgtcggcag ggactcctgc gcctgcccga
     1021 aactcgcgga agtagtctgt tgccctgtcg tctcccacct tccgtgcggc ttgcttcgcc
     1081 ggtgtgatgt acgacccgct gccaatccgc tcttctccat agttatcgac aactatcgct
     1141 cgcggttcac gaacgggaca cgcgtgttca caaagaccac accccatgca tttgtcggca
     1201 tgaaccaccg gacgattctg cgttccccgg tggtccgaaa ccaccaggaa ttcaatggcg
     1261 tcataaggac agacttcatc acagacaaga caggctttca gttcttccca tgcgacgcac
     1321 cgctccttca caatgcgcgc cgtgccgatc ttcgcgaacg atttctcctc gtgagaaagc
     1381 cttcgaatcg ccagtgtggg gcatatctgt gtgcacgcgt tgcacggctc ctcgcacccg
     1441 ccggacgcag gcaccagctt cggagtccac agcccttccc atccgatctc aaggccactt
     1501 ggctggagcc cgtgcgtggg gcaggcgtcc acgcagatca gacaccgggt gcaacgggaa
     1561 agaaattcct cttcgggaat cgcgccgggg gggcgaaggt gagcacccgg ccgcgtgctg
     1621 gctttcgtgg aggcatcgcc gaggaatccg gcggcgacgc ctgcgcccgc cgccagaagc
     1681 acctggcgcc tgcggggttc aaaaccacgt atctgcggtg gccccccgaa gacaaaccgg
     1741 acggtaccct tgctgcacgc acgttgacac ctcaggcact ggatgcactc gccacgccgg
     1801 gttttagtga aatcgttcac gggaatggca cccgtcgggc agtctcgctc gcatcgagca
     1861 catcgggagc aggagctctc cactgagcgc cgtaccaagc ccgtgcggga aaggagattc
     1921 agaaacgcgc ccaacgggca cagattgcgg caccagaacc gacgttcgat tacctcaaga
     1981 atcaggatcc ccgcaaagac ggcggctgtc agcgtgagga acccgtacgc cggcgggttt
     2041 tcgacggcac gaaacgcgaa cgattcctgc cccggtacca atctgaccgc tcgaactact
     2101 ccggtaccca cccaggcagc cgctggaatg aggacgagct cccgtgttcg agagaaaagg
     2161 acaagggggt caaaaaggac gaacgtggtt gtaccgaacg ccgccgcgac gatcaaaaag
     2221 gcgaggaaga cgaatttcca tcgcgcccgc gtaccgtttt cgcgcgtcgc atccttcgaa
     2281 ggccgtacac cgcagcgatg cgccgcgtcc agaaatgtgc caagcggaca caaccagcca
     2341 cagaaaaagc gcccgatgag aagtgttgcg ctggcgagaa gcagccctcg aagaacaagc
     2401 ccccacgaga attcgcgcga aacaagactt gcgattgaaa tgagagggtc atattcgagc
     2461 cagtaggttt ccgtacccgc gaggaatacc gcgaaggcaa ttaactgaac ggcgcgtcgc
     2521 aggttgttca ccgggtgcct caagtagcag gcgcggcgat cagagggtca ctcgaacgac
     2581 gttcattgcg gaaaggtccg tcagccctag gccctgttcc cccgccaatc gaagatgccg
     2641 gacctgatcg gcggtgaacg cgcggttgta ccaccgcgcc aggctcaccg tgtaggcatc
     2701 aactgtggtt tgattgcgcc ccgcaacaat ggcgttcggg gtctccacgc ggcccggccc
     2761 tcccggcccg cgggtcacca gcgccctgcg ggcgtcaacc acgaccagtt gtgctttgat
     2821 gcccttgctc aggtccacga tcgcctgatg gaggtcataa cgggcgtgaa aaggcccgcg
     2881 agagcgaatc agccccatca tccccttcat cgcgaacgag acgccggcgg aagaatggga
     2941 tttcgcaacg ggaactgcga tcagcacgtc actctggcgc acgaccgtgg caacttccac
     3001 ttccctggcg tcaatcccat cgggaatacg aagcggcacg tatgcgctcc cggaggtgag
     3061 gggaactaca ttcacgccgt tcatgcgcgc gaggtcggcg attccgctgc gttcgaaaca
     3121 aaggtccggt tcgcgaacgg ggtagctgga gactgtgatg gatgacgcac ccgcttcctt
     3181 gcacagggta gcgattgcgc ggatcgtctc aggatctgtg gtggttgcca tctctgcggg
     3241 gtttggaaat cccgcgttgg ggactatgag cactttctgg ccgcgccgta cgaaggcacc
     3301 gattcccccc agccgatcga tggaagtccg caccgccagt tcgacggttc caccggttac
     3361 gtgcaccaca tcgggtgaag tggccgcccg cgaaactgcc gaatacgccc atttcggcag
     3421 taccagcccg ccggctgcca tcgcgcctgc tttgaggaaa tcgcgccgcg aaagtcccat
     3481 gggaatgctc ctttttcctg ccacgccggt gcgcgcgacg gcgaacagcg ccgatgtaga
     3541 gtcgcgggcg ttgcagccct ggccggtcct atttcggaaa tacccggtgt aaggtaactc
     3601 cggagaaccc gtcaatcaca agccagagcc ctacgctgag aaattccccg agaataagac
     3661 caagggcccc gttcttcaat cggttgtagg cgcttgcacc gctgaaacgg ataatcgccg
     3721 ccttaatgac ccatcccaga aaaaccggga accagatttc attggacgcc acggtatcgc
     3781 cgatcaggaa acccagcggg taaatgggcc accagagaaa cgtgcgatgg agatacagca
     3841 gcacactcat gaacacggca cccgcgccca tcaggctgat tgccgtccag tgagtgtaca
     3901 cgtggtaacg ctgaaatccc gccgttcgat tgaaaagcca gtctccgccg ttgaagaaat
     3961 gccatggctc cagattcgcg ccaccatacg tgtatgccag ccagagaatc atcgcgaacg
     4021 agacaaaaac tgcggtgacg aggccaaggc caacgccaag gaagtagtgt ccgtgcctga
     4081 tgcttgtctg ccgcgcaatc ttcattccgt cgagaagctg gggcatgatg ttcgcccggt
     4141 tgctcaatgt ggaaaaatcc acaaacccca tgttcgtcca cgtccgaggg tctacgcggg
     4201 ccgaacccac gataaccgtc atgccgtgca tcggaacaaa gtagtcgtgc acccacagca
     4261 gccccgttgc cgcaacgata cgtgccgtcg tgattgcgat cacgaggaaa aagaacagca
     4321 acagaaaact caccgaaaac atgattccgg ccgccgctgt ccacgaaaaa tagattccta
     4381 cgcccaggag gaacgcccat agactccacc atggaatcga cgattcggca cccgaaccct
     4441 ttttcagtcg cttccgttga ccgaacgccg ccagaagcac ctcgcgaatt tccccgcgcg
     4501 acgcgatgaa atacaccagc atcgccagaa ccattgcccc gagacgctgg taacgagccg
     4561 caagggtata cccgaacgtg tggaggggtg tccactggaa ctggccgtca ccgatgctgg
     4621 tggattcgaa aatgatgtgc tcgatatagt aaaaccactc gaaaacccag acgctcaacg
     4681 acacttccga ccggagaagg tagctgattc cgactatcga ccagtaaaaa ttgaactgta
     4741 tgttcgccca gcggtaaaag ggaacgtccg tgaagacccg cgaaccgctg aaaagactcc
     4801 cgagtgtgat gttcgcgagg ccaattggag ggatggatgg gaaatagatg ttcaacccgt
     4861 tcacggtgtg aaagaagaac ggaattgcaa tgcctgccca caccagcggt tggcgaaaga
     4921 acgacgaata ccatgacggg gtcggatcat ctcccataac catcagtggt atctgcgcca
     4981 gtgggaactg caggcgttcg gcatccatcc atcgttttcg gaacgcgaca ctgagggaga
     5041 tcatgatgag acacagcgcg gccgcaagca gtatccagag cgccagcggc ttcacccatg
     5101 cttcccatgg aattgccgca ccggtcggtt tgccggtgaa gaaccagtcg attacctcct
     5161 gcccctgcgg cgtcatccag tgcggaacgt tcggctgatg cagtatcgcc cattcattaa
     5221 ttggagttgc gtagtaatac aactccacca gaaggggaac gagacgcgtg gccagtccat
     5281 cggaagggat ggccgaaggc acgagcatcg tcacgtatgc gagcatcagt tcctgctggg
     5341 tgaatgccat ccgggtgaat ctcagcgccg tattg
//
LOCUS       DAOTNG010000220         5367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_12832_length_5367_cov_5.177523, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000220 DAOTNG010000000
VERSION     DAOTNG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12832_length_5367_cov_5.177523"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="P5179_10395"
     CDS             complement(<1..741)
                     /locus_tag="P5179_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95670.1"
                     /translation="MEIPSSEQAREAIKLAVGRATERYVAQIAPEGETIKFPELLGKA
                     LPTMALRVLRSVEDSFWRKELERRKNDQRFDYADPALQTAVATWDAALRKCMVLSRKE
                     LHQLINRALSLQMESLVSPIDSLKANYFVRQEKVAAKSAVVLASHLGLEERYVRALSL
                     MAQDDEKRLLSADTVQRVFRDVDAKEFQGSKDRAAVSVLSNALLVLGIRAEDELGEAP
                     VDLALAVMVLRGTPEGTAKVAELCEGRAL"
     gene            complement(791..1279)
                     /locus_tag="P5179_10400"
     CDS             complement(791..1279)
                     /locus_tag="P5179_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRS95671.1"
                     /translation="MNKQGGSLSAEQMQTIESVLAGYVKDAGIRTVLLVDPDGHLVCQ
                     SGAMDTIDPVSFGALVSANFASTQQIAGQMGEKEFNAFYIQGKEKDLYVSAVEDKAIL
                     VSIFARGTTVGMVKIFAEKTVKEISANLVPGEGGDQKGLSVGGGFADSALAELDKVLG
                     GI"
     gene            complement(1331..1924)
                     /locus_tag="P5179_10405"
     CDS             complement(1331..1924)
                     /locus_tag="P5179_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRS95672.1"
                     /translation="MATINYGYREINCKIVYYGPGMSGKTTNLETIHKKVPPTNRGKL
                     TSLATKTDRTLFFDLLPLDLGTIKGFKVKFQLYTVPGQVYYNATRKLVLRGADGVVFV
                     ADSQKDRYDDNVESLENLRENLKEHGMDLDAMPFVIGWNKRDLPDVMSVEELNAKMNP
                     RGVPNFEMEALTGRGVFEALRAVTRMVLEKLEKEGTR"
     gene            complement(1956..2348)
                     /locus_tag="P5179_10410"
     CDS             complement(1956..2348)
                     /locus_tag="P5179_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95673.1"
                     /translation="MSEQVLEIVTPLAERPDVVRVVVSDREGLLIGALKGKAASGSMR
                     DEETTDEQWSAFVAQFVTDAGAQLKNITLHHPTDMVVHGTRDSLVITWLHVGWLITRV
                     SPVADWGDLFATVRAVQQQFDKVTGESA"
     gene            complement(2341..3042)
                     /locus_tag="P5179_10415"
     CDS             complement(2341..3042)
                     /locus_tag="P5179_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95674.1"
                     /translation="MPAALFDRLNDLQAAIEAENWADFDAVLKSWSAEEPENAWVSAG
                     LTLAKVLRGDEVPDTEASLQAINGRLAGMTGLLAAVRAGDKVEARKRALAELAAGNAD
                     QSLVETLKWADEAASDATRYLFATPGLVAGCVMAGGQPVSMTARGSRMSADSLGTKSE
                     AIWELAIKMNQFIDLGDLTGIRVFGEDGGWTLGAAYADDLRLVAGLVETASNMDELAA
                     RTRLLVAALEGPGNE"
     gene            complement(3042..4952)
                     /locus_tag="P5179_10420"
     CDS             complement(3042..4952)
                     /locus_tag="P5179_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS95675.1"
                     /translation="MLKSVLRLVPLYVLLRGTRIGGLITSGAKRMDAPQKKSNPPAPR
                     VGPGRADGVRLPEFLQKQVDAIDETAILNELIAAISPELLDLEPRTETVPAPAATPTP
                     PPAPAPREKQQSSPPREQKEEVEEPTAKSTTPLKGLQIRPEEVEEAISSILGPTEPQA
                     RVEKEKPAEKKEAPPPPKAEPAPTPPPEPAAAKAGPKVVAPPIKPPAKQEFDSVDDAL
                     SALWGKGEEEASTAVEPVAEAPAAAPTAAPPPPPVSEPAPPAPSPPAVEKAPTEPAKV
                     PAVEGGEIPPALVPGLARMAATHYLNLLGTVLPSGAAPPPAAEKAPPEPSAAPPPAET
                     PPTEEKPFAGLQEADVTADLLADFIVEEREGAGEEQKEAEPASLEEVVSSAILEPPVV
                     EEKAEEPVPVGQPEATAAGEEGEEGKPAPWTGPIADIGGDDVIAEMAKEHRRKKLIGW
                     VIVAFIAVAFASFFFGPKLKELISGLSTGTTGSPAAVTPAPTPQPAPAADSVAQPAPP
                     PAPAPAAEATTPAPPPAVAAPAPAPAPPAPRPSGGAPQLPTSPGGRFTVHVESLPTLG
                     EANAAKSKWQRRGYEALVWPFTKNDGSQWYRVGVGRFATKADGQTAKSALESAFPEID
                     WSQVTQIPEGAN"
BASE COUNT          939 a         1702 c         1547 g         1179 t
ORIGIN      
        1 aagagcgcgg ccctcgcaca gctccgcgac tttggccgtt ccttccggcg tgccccgcag
       61 aaccatcacc gcaagtgcca ggtccaccgg agcttcaccg agttcgtctt ctgcgcggat
      121 gccaagaacc agcagcgcgt tgctgaggac cgacaccgca gcgcggtcct tcgaaccctg
      181 gaactccttg gcgtccacat cgcggaatac cctttgaacc gtatccgcgg agagaagcct
      241 tttttcgtca tcctgtgcca tcaacgagag cgcgcggacg tagcgctctt cgagaccgag
      301 gtggctggca agcacgacgg cgctctttgc ggcaactttt tcctgccgga cgaagtaatt
      361 ggctttcagg ctatcaatcg gtgaaaccaa agattccatc tgcagagaca gcgcccggtt
      421 gatcaactgg tgcaactcct tgcgcgaaag caccatgcat ttccgcaggg ctgcatccca
      481 ggttgccacg gcggtctgga gagcagggtc ggcatagtcg aagcgctggt cgtttttccg
      541 gcgttccagt tcttttcgcc agaagctgtc ctccacagag cgaagaaccc gcagcgccat
      601 tgtgggcagt gctttgccaa gcaattcggg aaacttgatg gtttcccctt caggcgctat
      661 ctgcgcgacg taacgctccg tcgcccttcc aactgccagt ttgatggctt cgcgcgcctg
      721 ttctgaagat gggatttcca tgatcaacct ctctacggcg cagttgcgcc acacacgcgg
      781 agttgcacgc tcagataccc cccagcacct tgtcgagttc tgccagcgcg gaatcggcaa
      841 acccgccccc gacgctcaaa cccttctgat cacccccttc gccggggacg agattcgccg
      901 agatctcctt caccgttttt tccgcgaaaa tcttcaccat tccaacggtt gtgccgcgcg
      961 caaaaatcga gacgagtata gccttgtctt ccaccgcgct tacgtacagg tccttctcct
     1021 tgccctgtat gtagaacgca ttgaactctt tttcacccat ctggccggcg atttgttgag
     1081 tcgaagcgaa gttggccgac accagcgcgc cgaacgatac gggatctatc gtatccatgg
     1141 cacccgactg gcatacgagg tgtccatccg ggtccacaag cagcaccgtc ctgattccgg
     1201 cgtctttcac gtaacccgcc agaacactct caatggtctg catttgctcc gcggagagag
     1261 agcctccctg tttgttcatc gtctatgctc catctgcggc gatgggtgga tgagcagtga
     1321 ccgcgccggg ttatcgcgtg ccctcttttt cgagcttttc cagaaccata cgcgtaaccg
     1381 ccctcaatgc ttcgaacact ccgcgccccg tcagcgcttc catttcgaag ttcgggacgc
     1441 cccttggatt catcttggcg ttcagttcct ccaccgacat cacgtcaggc agatcccgtt
     1501 tgttccagcc gatcacaaac ggcatggcat cgaggtccat accgtgttcc ttgagattct
     1561 cccggaggtt ttccagactt tccacgttgt cgtcgtaccg atccttctgg ctgtccgcga
     1621 caaacacgac accgtcggca ccacgcagta caagctttcg cgtggcgttg taatacacct
     1681 gcccggggac tgtgtaaagc tggaacttca ccttgaaacc tttgatcgtt cccagatcga
     1741 gtggtaacaa gtcaaagaac aatgtccggt ccgtttttgt cgcgaggctt gtcagcttcc
     1801 ctctgttcgt cggggggacc tttttgtgta ttgtctccag attcgtggtt ttgccactca
     1861 taccgggacc gtaatagacg attttgcagt tgatttcccg gtagccgtaa ttgatcgttg
     1921 ccatgaatca ccctcccacg aaacctgaat gtgtgtcaag ccgactcgcc ggtcactttg
     1981 tcgaattgct gctggactgc acgcacggtt gcaaagaggt cgccccagtc cgcaaccggg
     2041 ctgacacggg tgatcagcca tccgacatga agccacgtga tgacgaggga atcccgcgtg
     2101 ccatgaacaa ccatgtccgt ggggtggtgc agcgtgatgt tcttcagttg cgcccctgca
     2161 tcggtgacga actgggcgac aaatgcgctc cattgctcat cggtggtctc ctcgtcacgc
     2221 atggaacctg acgccgcctt gcccttcaat gcgccgatca gcaggccttc ccggtcagac
     2281 acaacaacac gcacgacgtc cgggcgttcc gcgagaggag tgacgatttc cagtacctgt
     2341 tcactcattg cccgggccct ccagcgcggc gacgagcagg cgggtgcggg ccgcaagttc
     2401 gtccatattg gatgcggttt ccaccagacc ggcaacgaga cgcaagtcgt ccgcataggc
     2461 ggcgccgagc gtccatcctc cgtcttctcc gaacacacga atacccgtca ggtcgccgag
     2521 atcaatgaac tggttcatct tgattgccag ctcccagatg gcctcagatt tcgtgcccag
     2581 cgaatccgct gacattcggg aaccgcgtgc cgtcatcgac acgggctgcc cgcctgccat
     2641 cacgcatccg gcaacaagcc ctggtgtcgc aaacaagtac cgggtggcat ccgatgccgc
     2701 ctcatccgcc catttgagtg tttctacgag cgattggtcg gcgttgcccg ctgccagttc
     2761 cgccagcgct cgttttctgg cttccacctt gtcacccgca cgaacggctg caagcaagcc
     2821 agtcataccg gcaagccgtc cgtttatggc ttgaagggat gcttccgtgt cgggcacctc
     2881 atctccgcgg agcactttcg ccagcgtaag gcccgcgctg acccaggcgt tctctggctc
     2941 ttccgcagac caggacttca gaacggcatc aaaatcggcc cagttctccg cctcgattgc
     3001 cgcctgaaga tcattcagtc ggtcgaataa cgctgccggc atcagttggc tccttcggga
     3061 atctgggtaa cctgtgacca gtctatctcg ggaaacgcgg attccaaagc gctttttgcc
     3121 gtctggccgt ctgccttggt ggcaaagcgc ccgacgccga cgcgatacca ctgtgaaccg
     3181 tcgtttttcg tgaacggcca cacgagcgcc tcgtaccctc tccgctgcca tttcgatttc
     3241 gcggcattgg cttcaccgag agtcgggagg gattccacat ggaccgtgaa ccgtcccccg
     3301 gggctggtgg gtagttgggg cgcgccgccg gagggtcgtg gcgcgggagg tgctggtgcc
     3361 ggcgcagggg cagccactgc cggcggaggc gccggagtcg ttgcctccgc agctggtgcc
     3421 ggcgccggtg gcggagcggg ttgggcgaca ctatccgcgg cgggtgccgg ttgcggagtc
     3481 ggtgccggag tgacggccgc aggcgatccg gtcgtaccgg tcgagaggcc ggagatcaat
     3541 tccttcagtt tcggaccgaa gaagaaggag gcaaacgcca ccgcgatgaa tgcaacgatt
     3601 acccacccga taagcttctt tctccggtgc tctttcgcca tctccgcgat gacgtcgtcg
     3661 ccaccgatgt cggcgatggg gcctgtccat ggcgcgggct ttccttcctc accctcttca
     3721 ccggctgcgg tggcttcggg ttggccgacg gggacaggtt cctccgcttt ttcctcaaca
     3781 acaggaggct cgagtatggc ggaggagacc acttcctcca gcgaagcagg ttcggcttcc
     3841 ttctgctctt cgcccgcacc ctctcgttcc tcgactatga aatccgccag caaatccgcc
     3901 gtgacatcgg cttcctgcag gccggcaaac ggtttctcct cggtcggggg tgtttccgca
     3961 ggcggtggag cagccgatgg ctccggcgga gccttttccg cagcaggagg aggtgccgcc
     4021 ccgctcggaa gaactgtccc gaggaggttg aggtaatgtg tcgccgccat ccgcgcgagg
     4081 cccggaacca atgcgggggg gatctctccg ccctccaccg cgggaacctt ggcgggctcg
     4141 gtcggagcct tttccacggc aggcggagaa ggcgcgggag gcgctggctc agacaccgga
     4201 ggagggggcg gagccgctgt gggtgccgca gccggcgcct ccgcgacagg ttccacggcg
     4261 gtgcttgcct cttcctcacc tttgccccag agcgccgaca gcgcgtcgtc taccgagtcg
     4321 aattcctgtt tcgcaggcgg cttgataggc ggggcaacta ctttcgggcc cgctttcgcc
     4381 gcggctggtt ctggtggagg agtcggcgct ggctcggcct tcggcggagg tggagcttcc
     4441 ttcttttccg cgggtttctc tttttccact ctggcctgcg gttcggtcgg gccgaggatg
     4501 gaggaaatcg cctcctccac ctcctctggc cttatctgga ggcctttcaa aggcgtggtc
     4561 gattttgcgg tcggctcttc aacctcttcc ttctgctccc tgggcgggga cgactgctgt
     4621 ttttcccggg gcgcaggggc tggcggaggt gtcggtgtgg cggcaggtgc aggaaccgtc
     4681 tccgtccggg gttcaagatc cagcagttcg ggcgagatgg cggcgatcag ctcgttgaga
     4741 atcgccgttt catcaatcgc atccacctgc ttctgaagga attccggaag cctcacgccg
     4801 tccgcccggc ccggcccgac gcgcggagcc ggagggttcg atttcttctg tggtgcatcc
     4861 atgcgctttg ccccgctggt aataagccca ccgatccgcg tccctcgaag caacacgtag
     4921 aggggaacaa gacggaggac acttttcagc atctggaacg atgcacagtg ctcgcgcgct
     4981 ccattccttt gccacgtgtt cctcaaccgt tcaatgtatg gttttttttg ttgtctgtct
     5041 accccctccg taccgaaaca aaagaaattc cgtacttacg cagttcacat tcgggttttg
     5101 ttctcgacga acctgtattc tgaacgggca agaatcccct gtcacagatc tggcacggag
     5161 ctctcctcaa agcgctccga cagcattccc gcaccttgta cgctggtttc cggaagtggt
     5221 gccggtgcga ttgggaggtc ccgaacgtgg tgttccaccc ggcaccttcg cctcatgttg
     5281 cgctgtacca gcgggaatag ctcccgcccg tgcacggcca gacggtctgg gcggcgattc
     5341 ccggggtgcg gatgcagtac aacgatc
//
LOCUS       DAOTNG010000221         5298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13017_length_5298_cov_4.271982, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000221 DAOTNG010000000
VERSION     DAOTNG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13017_length_5298_cov_4.271982"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="P5179_10425"
     CDS             <1..699
                     /locus_tag="P5179_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95676.1"
                     /translation="AVFSLLFAFSVVPKASTLSMMFFVVPQGIAKLPHFVVGFGMLFF
                     FLLFIAGLTSSISLVEAFVASILDKFRWRRKKAVLVVALCSMLGSMCFALPHVVDPTL
                     ASNGTLGLTLLDLFDHWSFSYGLLIVGFFECLIIGWVFGARKLREIINRDSTLGIGWW
                     WDALIKVVNPVIIAVVIGWGVWTEVRDGLYGASFTIQGIPWLSWFAFGAWFLFTTGLA
                     LVFTFGRKYREEQG"
     gene            696..1196
                     /locus_tag="P5179_10430"
     CDS             696..1196
                     /locus_tag="P5179_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95677.1"
                     /translation="MNRLVFWVPVFAFISVSTARGIVLSVEPASPVRGEVAAIRVFGD
                     ATADTSLAAREAARTGWKVYVEYFPNSKVARDDTLGVTGPAGEVFWEPAFAGIVAVRA
                     IRKKEGGSPEQLATNVSVRFPSVPAGGVLVFLTAGFVLLGGAGWAFVHLMEHKPPGEG
                     RSEPEA"
     gene            complement(1462..2587)
                     /gene="prfB"
                     /locus_tag="P5179_10435"
     CDS             complement(join(1462..2529,2531..2587))
                     /gene="prfB"
                     /locus_tag="P5179_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRS95678.1"
                     /translation="MPLVNTDRLTELRERLDSLGGFFDLARKREQISILEQQMAAPGF
                     WDDGQAAQKTISAANELKEWIQDWESLDRRLNDLAELAELAELEEDDAVAAEVERDTE
                     TLANDITALEFRHMMSDPADKKNAIVTIHPGAGGTESTDWAQMLMRMYTRWAERKGFQ
                     VEIMDLLENEDAGIKSVTLEVTGPYAYGYIKAESGVHRLVRISPFDSQKRRHTSFASV
                     FVYPELDDEIKLDVKESDIRVDTYRSSGAGGQHVNKTSSAVRMTHLPTGIVAQCQNER
                     SQHKNRANCWKILMGRLYEHYKALEDEKLQAVEDTKKPIEWGSQIRSYVFQPYQLVKD
                     HRTGIETSNVDAVMDGDIDLFINGYLTRKGDLTPDDSLAQ"
     gene            2896..4671
                     /gene="carB"
                     /locus_tag="P5179_10440"
                     /pseudo
     CDS             2896..4671
                     /gene="carB"
                     /locus_tag="P5179_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            4734..5117
                     /locus_tag="P5179_10445"
     CDS             4734..5117
                     /locus_tag="P5179_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95679.1"
                     /translation="MSGSWERLGVHLMNQPFFLPAVDEAAEHETAGLSGGLQPRCVSC
                     KACREPVWVVSDSRGVSFALSRASYIGGEAAPVAWAPAVVFPSAEALKHARETGGDRK
                     QAVSCPFCGEHLAVPTPPEDMNSRH"
BASE COUNT          972 a         1591 c         1600 g         1135 t
ORIGIN      
        1 gcggtgtttt cgcttctttt cgcgttctct gtggtgccga aagccagcac tctcagcatg
       61 atgtttttcg tggtgcccca gggtatcgcg aaactgccgc attttgtcgt gggtttcggg
      121 atgctgtttt tcttcctgct gttcatcgcc ggcctcacct ccagtatctc cctcgtggag
      181 gcctttgtcg cgtccattct cgacaaattc cggtggagga gaaaaaaagc cgtactggtc
      241 gttgcgctgt gcagtatgct gggtagcatg tgcttcgctc ttcctcacgt ggtagatccc
      301 acccttgcca gcaacggcac cctcggcctc acgctcctcg atctgttcga ccactggtcg
      361 ttcagttacg gcctcttgat tgtcgggttt ttcgagtgtc tcatcatcgg gtgggtgttc
      421 ggtgcacgca aactgcgcga aatcatcaac cgcgacagta cgctcggcat aggatggtgg
      481 tgggatgcgt tgatcaaagt ggtcaatccc gtcattatcg cggtggtgat cgggtggggc
      541 gtctggacgg aggtgcggga cgggctctac ggcgcttcat tcacgatcca gggaataccc
      601 tggctttcgt ggttcgcgtt cggggcgtgg ttcctcttca ccacgggtct cgccctcgtg
      661 tttaccttcg ggcggaagta ccgggaggaa cagggatgaa ccgactggta ttctgggtcc
      721 ccgtattcgc gttcatcagc gtttcaacgg cgcgcggcat tgtgttgagc gtcgaaccgg
      781 cgtctccggt acggggcgaa gtcgccgcaa tccgggtttt cggtgatgcc acggccgata
      841 cctcactcgc cgcccgggaa gccgcccgca ccggctggaa ggtttacgtt gagtacttcc
      901 ccaacagcaa ggtggcgcgg gacgacacgc tcggcgtcac cggtccggcg ggagaagtgt
      961 tctgggaacc cgcattcgcg gggattgtcg cggtgcgggc gataagaaag aaggagggtg
     1021 gctcccccga acaactggcc accaacgtaa gcgtgaggtt cccctccgtt ccggcaggtg
     1081 gagttcttgt gtttctcacc gccggattcg tcctccttgg cggtgccggc tgggcgtttg
     1141 ttcacctgat ggaacacaaa ccgccggggg agggacggtc agaacccgaa gcgtaagccc
     1201 cctgccgcac ggcaagcccg cgcccggcac cgcgccgtca ttcccgcgaa ggcggggatc
     1261 cagccggtgc gccttttttc cttccgccac aaaaggttcc ggaatccgtt tccctcgcgg
     1321 ccggggcgcc cacgaattac tcacccaatc cccgtggagt gtttccggat tcccgcctcc
     1381 gcgggaatga ctatcgtggt gcctctccga ctgcgcgccc gtcgaatcag atccatgcgc
     1441 cccggtgcca gacctcgcct gctactgcgc cagcgagtca tccggcgtca gatccccttt
     1501 gcgagtcagg tatccgttga tgaacagatc aatgtctccg tccatgacgg cgtccacgtt
     1561 ggatgtctcg atgcccgtgc ggtggtcctt gaccaactgg tacggctgaa acacgtacga
     1621 ccgtatctgg ctgccccatt cgatgggctt tttggtgtct tcgaccgcct ggagtttctc
     1681 gtcttccagc gccttgtagt gctcgtacag gcgacccatg aggattttcc agcagttcgc
     1741 gcggttcttg tgctgcgagc gctcattctg gcactgcgcg acgataccgg tggggaggtg
     1801 ggtcattcgt accgcggagg aggtcttgtt cacatgctgc ccccccgcgc cggacgagcg
     1861 atacgtatcc acgcgaatat cggattcctt cacgtccagc ttgatttcgt cgtcgagttc
     1921 cgggtacacg aacacactgg cgaatgaggt gtgccggcgc ttctgggaat caaaaggaga
     1981 gatgcggacg agccggtgca cgccgctttc ggccttgata tacccgtagg cgtacggccc
     2041 ggtcacctcc agcgtcacgc tttttatgcc ggcgtcctcg ttttccagca gatccatgat
     2101 ctcgacctga aagcccttgc gctccgccca ccgggtgtac atccgcataa gcatctgcgc
     2161 ccagtcggtg gattcggttc cgccggcgcc ggggtggatc gtcacaatgg cgtttttctt
     2221 gtcggcgggg tcggacatca tgtggcggaa ttcaagcgcg gtgatgtcat tcgccagcgt
     2281 ttcggtatca cgttcgacct ccgccgccac tgcgtcatcc tcttccagct ccgccagttc
     2341 ggcaagttct gcaaggtcgt tgagtcggcg atcgagtgac tcccagtcct gaatccattc
     2401 tttgagttcg tttgcggcgg agatggtttt ttgtgcggcc tggccgtcgt cccagaatcc
     2461 cggtgcggcc atctgctgtt cgaggatgct tatctgttcc cgtttgcggg cgaggtcaaa
     2521 gaaacctccc gaggctgtcg aggcgttcgc gaagttcggt aagccgatca gtgtttacca
     2581 gtggcatgtg tcaatcctcg tggtgaatca agaggtgaat cgtccgcgtc ccggggtttt
     2641 cactcgttcc aaaccctttt ccacggaaac ccgcggcacg tacagaaggt acgtcgcgcg
     2701 ggcctgcaga gcacgtccgg aaactgctcg ttgacgcttc atgcctcctg aggtaccttt
     2761 agatgccctg gtttttcgcc ctttgcgggc ctgtgctgcg ttctcgcccg cgctctcgct
     2821 ttctttcagg ttgccaccat gccggttcca gattcccccg tagagcggct caccgcacgc
     2881 atcccacgcg cgacgcttct cagggccaag cagatggggt tcagcgacat acagcttgcg
     2941 cacctttgga acgtcgatcc tgcggacgtg cgcgagcttc ggaagaagct cgacgtcctc
     3001 ccgacattca aaacggtgga cacgtgcgcg gcggagttcg aagcccgaac cccgtaccac
     3061 tacagcacgt acgaaaccga gtgtgaagta gtccgttctc cgaaaaagaa gatcatgatt
     3121 ctgggtggcg gaccgaatcg aataggccag ggcatcgagt tcgattactg ctgctgccac
     3181 gcggcgtttg cgctgcggga agacggtttc gaaaccatca tggtcaactc gaacccggaa
     3241 accgtttcga cggattatga tacctctgac aagctctact tcgagccgct caccgttgag
     3301 gacgtgctga acatccatga cagggaacaa cccgacggcg taatagttca gttcggcggg
     3361 cagacgccgc tcaaactcgc cgttcctctg gagaaggcgg gagtaaaaat catcgggacg
     3421 tcgccggcga tgatagacgt cgcggaggac cgcaagctct tcggcgcgct tatcaaccgg
     3481 ctcggaatcc gccaaccgga gaacggaacc gcacgcagcg aggaggaggc ggaagccgtg
     3541 gcaagccaga tcggctaccc ggtgctggtt cgtccttcct acgttctggg tggccgcggg
     3601 atggccatcg tgtacgaccg ggattcgctg gtgcattata tgagagccgc ggtgctggct
     3661 tctcctgaac accccgttct gattgaccga tttctcgacg atgccatcga aatagatgta
     3721 gacgccgttt cggacggtac gctgacggta gttggcggaa tcatgcagca gatcgaactg
     3781 gcgggagtcc attccggcga ttccgcgtgc gtgctgcccc cgtaccgcct caccccggac
     3841 atgatcgaaa agttgatgcg gcatacgaag gaccttgcgc gcgaacttca cgtcgtgggg
     3901 ctgatgaacg tgcagtattc catcaaaggc gacgaggtgt acgttcttga ggtgaacccc
     3961 cgcgcgtcgc gtacggtgcc gtttgtcagc aaggccatcg gtgtaccgct ggcgaagatt
     4021 gccgcccgct gcatggtggg caaaagcctt gccgaacagg gcttcacgtc ggaaattgtg
     4081 ccgccgtacg tctccgtcaa ggaagccgtg ctgccatttc tcaagttccc cggcgtggat
     4141 atcctgctca gcccggagat gcgttcgacc ggcgaagtga tgggtatttc agaatccctt
     4201 ggagagagct acgcgaaatc ccaactcgcg gcaaatcttc gcctgccgga caggggaact
     4261 gttttcatca gcgtgggcgg agacgagcag aagcgagctc ttgtggagcc tgcgcggcgg
     4321 cttgccgaac tcggtttcga catcatcgcc accgaaggca cgctggcaat gctccatcgg
     4381 aacggcgttc ccgccacgtt cgttccgaaa ttgcaggaag gccgccccca cgtggttgac
     4441 atgatgaaat cgtccaagat cgcgcttgtg ataaatgtgc ctcacgggaa agccgcgtct
     4501 caggatgaaa tcgccatccg acgcgagacg atccggcgcg gccttcccta catgacaacg
     4561 gttgccgccg cggtcgccgc ggtggagggg atcgcagccc tgcgcgcgga ggaaatgagc
     4621 gtgcgttccc tgcaggagca tcacgcccgc atacatcgcc cgacgcaatg agtgcacgac
     4681 aacgaaccag agttctcgga tctccgcctc gctccgggaa gccgctcgtc ggcatgagcg
     4741 gctcatggga acggctcggc gtgcatctga tgaaccagcc gttcttcctc cccgcagtgg
     4801 atgaggcggc cgaacacgaa acggcaggcc tctccggtgg cctgcaaccc cgatgcgtat
     4861 cctgcaaagc atgccgggaa ccggtatggg tagtttctga ttcccgcggc gtctcgttcg
     4921 cgctctcccg cgcaagttac atcggtggtg aagcagcgcc cgtcgcctgg gcgccagcag
     4981 tggtgtttcc aagtgcggaa gccctgaagc acgcgcggga aacaggcggc gaccggaaac
     5041 aggccgtcag ttgccccttc tgcggagaac accttgccgt cccaacgcct cctgaagaca
     5101 tgaactcccg tcactagaac cagaggaata catccgcaca tgagcagcga gtcgaacaag
     5161 ggtgagaatc cggctccttc gacacgagcc acaaggggta tcgtgggatg ggtcagacgc
     5221 atcgtatggc gccgaaatgc ccgcaggatg aaggaatccg acattgatct gatgcggaaa
     5281 cggatcataa tcatcacc
//
LOCUS       DAOTNG010000222         5281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13064_length_5281_cov_4.814007, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000222 DAOTNG010000000
VERSION     DAOTNG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13064_length_5281_cov_4.814007"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="P5179_10450"
     CDS             complement(<1..948)
                     /locus_tag="P5179_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95680.1"
                     /translation="MMTSEERVLAACAFHRPDRVPRFDSFWDLPDAWRQEIGELKDLT
                     DIQIWAADETPFPSRKTVLKEQDGWLYERDGWGSTFRTRPGTYFREVLEVAIPEGVDP
                     DRVTFEDPASDRRYLMGRQTWSELRQELDSARREYCVFAKTGGPFLRTTLLRGEKQFL
                     MDIATDEVLARNLAEKVGSHLCDVGVESITRWGLTNTGIWIYDDVAFNEAPLFSPSAF
                     ERVFLPVYSQMISRYKAAGAKYVFFHSDGNINPFVDMLVNAGISGLHPLERRAGVDPF
                     LLRERYPKLVLAGGMCNTDILINGPVDRIEVEARKLIELA"
     gene            1173..2006
                     /locus_tag="P5179_10455"
     CDS             1173..2006
                     /locus_tag="P5179_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95681.1"
                     /translation="MRLPEIDFGPHRISRLIVGGNPLRGNSHLTPERNAEMRDYYTTE
                     RVLDAWFASEKAGVTAMQSRGDQIVMDWVDRYRERGGTMHWIVQTASEWKGGDVADNI
                     RAVAEHHPIAIYHHGTRTDNLWKQGRIDDVHDCLKQIHDKGLLAGVGSHMPEVCEYIE
                     EHDWEVDFYMTCAYNLSREERESFLVNGSTGTEVYDDADRDRMTDFVLSTPKQCILFK
                     ILAASRKCATQESVKEAFAYAFRRIKPCDAVDVGVFQRDRDEIGLDAEYVREVTGFAA
                     S"
     gene            2029..3123
                     /locus_tag="P5179_10460"
     CDS             2029..3123
                     /locus_tag="P5179_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95682.1"
                     /translation="MRSKADDFPCPICGEVLRAKLVERVDADSDGESLALLMAGRLNR
                     LLCPVCGHGGVAPEPVLVHSDAHRTCAAFVPFTIPANRKSHLAGKFKGLAQRVAGQYG
                     QAHVCFGLKQLREALPWLPAFPNSARGFQRFDAPGSAFHQVHALRRALKNRGGDVRLL
                     ARLGTALYASGDLKGARKTMETVVREEPDNGDAWRCLGAILLDSGQARPAAEAFERAY
                     RHHRDPECLFLAGVAAFRAGAPLEALTRFQRVIQKRADFFHAYVWIAVVHTAEGRYGP
                     AIEALRAAVMNGLKDAGMIRSRKELSPLFGERGFQSLMKHLEKPQARGLNRPSTTKRG
                     TGSGRPRAKRSGRRMPTSPVQKRRQAVKGK"
     gene            3137..4138
                     /locus_tag="P5179_10465"
     CDS             3137..4138
                     /locus_tag="P5179_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRS95683.1"
                     /translation="MRRAYVLFALLALFCGAGQASPPIRVAVTLTDFLPIVEAIGGSQ
                     VEAFSIMPPGSDPHSVSLNTEIIQRVREAQIAVYAHSASLAFEHDLKNSLAGKPSVDW
                     PQYEQQGARLLDFPGMPSNPHGFWLDYDNAVAIARAVASELTKMSVDEKVVAANLKRF
                     SDEVHAQREAVLQAMRDCGSLGDTVVAVVPGVAYTVRNLGYAVGGILLPDEGSGQADA
                     KSLMQISRLLGKGGARLLVCPVGLRNAKAGEIAEQLAKDTGTRVAYTRFLQTGGTNRT
                     FLGDAAYNAMVTSTVLVDVQPEAGGLANQSIYPTLLAIMGGVFLLLIFLVIRRILSR"
     gene            4146..4952
                     /locus_tag="P5179_10470"
     CDS             4146..4952
                     /locus_tag="P5179_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRS95684.1"
                     /translation="MADVADDRNPAVEVRELSYSYQRPLVEALRGVSLTLPRGTFTAV
                     MGPNGSGKSTFLRLLLGILRPTRGEIRVLGESPTAPSQRVQRLIAYVPQYDSINPLLP
                     LRSKEVIALGLSSRVTDRPARWIDERVSQILEDLDLVEIRDHQYGLLSGGQKQRVLLA
                     RALALEPELLVLDEPFSAMDMTSQHATAKLLSGLVRQKGKTVITVVHGIHTIVHHLDN
                     LVLLNREIVAAGTPDEVLRPANLTRAYGTDVPVVICPEGFRHPLVEDVHA"
     gene            4945..>5281
                     /locus_tag="P5179_10475"
     CDS             4945..>5281
                     /locus_tag="P5179_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRS95685.1"
                     /translation="MPDSVSYLTSPFVLRAAAAFFFVGINAALSGVFANFRNSTFLVS
                     GASHAALGGAALVIVLESRGSAFGISPVFGAAVFAVLLALAAGSARNRGSTAGSDTAI
                     GVGFALSMAL"
BASE COUNT         1083 a         1525 c         1606 g         1067 t
ORIGIN      
        1 cgcaagctcg atcagttttc gcgcttccac ctcaattcga tccaccggcc cgttgatcag
       61 gatatccgta ttgcacattc cccctgccag gacaagtttt gggtagcgtt cccggagcag
      121 aaaaggatcc acgccggccc gtcgctccaa cgggtgcagg ccgctgatgc ccgcattaac
      181 aagcatgtcc acgaacgggt ttatgtttcc gtccgaatgg aaaaagacgt actttgcgcc
      241 ggcagctttg taccgcgaaa tcatctggct gtacacaggc agaaaaacgc gttcaaaggc
      301 ggacggactg aaaaggggag cctcgttgaa cgccacgtcg tcgtaaatcc agatccctgt
      361 gttcgtcagc ccccatctgg tgatgctttc cacacctaca tcgcagaggt gtgatcccac
      421 cttctccgcc aggttccgag cgagaacttc atcagtcgcg atgtccatca ggaactgttt
      481 ttccccgcgc agaagtgtcg ttcgcaagaa cgggccccct gtttttgcga aaacacaata
      541 ctcacgccga gcgctgtcca gttcctgccg caattcggac caggtctggc ggcccatcag
      601 atagcgtctg tcgctcgcag ggtcttcgaa tgttacgcgg tcgggatcga cgccctccgg
      661 aatcgcaact tcaagcacct cgcggaagta agttcccggc cgcgtccgga acgtacttcc
      721 ccacccgtcg cgctcgtaga gccacccatc ctgttccttc agcaccgttt ttcgagaagg
      781 gaacggcgtc tcgtccgcgg cccagatctg gatgtccgtc aagtccttca gttcgccgat
      841 ttcctgacgc cacgcatcgg gcaagtccca gaaggaatca aaccgcggaa ctctgtccgg
      901 gcgatgaaac gcgcacgcgg caagcacacg ttcttccgac gtcatcattc cagttcccta
      961 tcgaaaatcg aatgcacccg tgagatcagg cggacactcc ttgcataccg ggtatgcgcg
     1021 cccggagcac cacttccgtt ctgcaactcg ctcctgccgt cgagcgccgg caaagatgcc
     1081 gacgggacgt cgagagcgta cattcattta ccacgggcat caccagtggc acaagggaag
     1141 acgacctgga tttcaagaag gaggtttggg cagtgcgttt gccagagatt gatttcggtc
     1201 cgcacagaat ttcccgcctt atcgtcggtg gaaaccctct gcgcgggaat tctcatttga
     1261 cgcccgaacg gaacgccgag atgcgcgatt actatacgac tgaacgtgtt cttgatgcgt
     1321 ggtttgcctc ggaaaaggcc ggcgttacgg cgatgcaatc tcgcggcgac cagatcgtga
     1381 tggactgggt cgaccgttac cgggaacgtg gcggcacgat gcactggatt gtgcaaacgg
     1441 cgagcgaatg gaaaggcggc gacgtggcgg ataacatccg agccgttgcc gagcatcacc
     1501 cgatagcgat ttaccatcac gggacacgga ccgacaacct ttggaagcaa gggcgcattg
     1561 acgacgtcca cgattgtttg aagcagattc acgacaaagg gcttctggcg ggggtgggat
     1621 cacatatgcc ggaagtctgc gaatacatcg aagaacacga ctgggaagtc gacttctaca
     1681 tgacatgcgc gtacaatctc agccgcgagg aacgggagag ttttctcgtc aacggaagca
     1741 cgggtacgga ggtgtacgac gacgcggacc gggaccggat gacagatttc gtgctttcca
     1801 cgccgaagca atgcatcctg ttcaaaatcc tcgccgcaag ccggaaatgc gcaacgcagg
     1861 agagcgtgaa ggaggcgttt gcgtacgcgt ttcgccggat caaaccgtgt gacgcggtgg
     1921 acgtgggggt gtttcagcgg gaccgcgatg aaatcggatt ggatgcggaa tacgtgcgcg
     1981 aagtgaccgg attcgccgct tcatgacgtc gccacgaacg ggacagtgat gcgatcgaaa
     2041 gctgatgatt tcccatgccc catctgcggc gaggttctcc gggcgaaact ggtggaacgc
     2101 gtcgacgcag attccgatgg ggagagcctg gcgctcctca tggcggggcg gttgaaccgg
     2161 cttttgtgcc ccgtgtgcgg gcacggcggc gtggccccgg agcctgtgct ggttcattca
     2221 gacgcccaca ggacatgtgc ggcgttcgtc ccgttcacga tccctgccaa cagaaaatcg
     2281 caccttgcag gcaaattcaa agggcttgcc cagcgcgttg cggggcaata tggccaggcg
     2341 cacgtatgtt tcgggctcaa acaactccgg gaagctctgc cgtggttacc ggcattcccg
     2401 aactccgcac ggggtttcca gcgttttgat gcacccggtt ccgcgtttca ccaggtgcat
     2461 gcgctccgcc gtgcgctcaa gaatcgggga ggtgacgtcc gattgctggc tcgcctggga
     2521 acggcgctgt acgcttccgg ggatttgaaa ggagcgcgca aaacgatgga aaccgttgtc
     2581 cgcgaagagc ccgacaacgg agatgcgtgg aggtgtctgg gtgcgatact tctggattct
     2641 ggacaggcga ggcccgcagc ggaggcattc gaacgcgcat acaggcatca tcgtgaccct
     2701 gaatgccttt tccttgccgg agtggccgct ttcagggcag gggcgcctct ggaggcgttg
     2761 acgcggtttc agcgggtgat tcagaagagg gccgatttct tccacgcgta cgtctggatt
     2821 gcagtggtac acaccgcgga agggagatat ggaccggcca tcgaagcgct tcgcgcggcg
     2881 gtgatgaacg ggctcaaaga cgccgggatg atccgttcac gcaaggaatt atccccgctt
     2941 ttcggagagc gtggattcca gtctctgatg aaacacctgg aaaaaccgca ggcgcggggc
     3001 ctgaatcgtc cgtccaccac gaagcgagga acgggcagcg gccgaccccg cgcgaaacgt
     3061 tcgggccgaa ggatgccgac gagtcccgtt cagaagcgca ggcaggcggt caaggggaag
     3121 tgaggaggac aaaactgtga gacgtgcgta tgtgttgttc gccctgctcg ccttgttctg
     3181 cggagccgga caggccagcc cgcccatccg cgtggcagta acactgacgg atttccttcc
     3241 catcgtggag gcgatcgggg gcagtcaggt ggaagcgttc agcatcatgc caccggggag
     3301 tgaccctcac agcgtatcgc tcaatacgga gattattcag cgcgtccgcg aagcgcaaat
     3361 tgccgtttac gcacacagcg catcactcgc cttcgaacat gatttgaaga actcgttggc
     3421 aggaaagccg tccgttgatt ggccgcaata tgaacaacag ggggcccggt tgctggattt
     3481 ccctggaatg ccctcgaacc cccatggatt ctggctggat tacgacaacg ctgtggcaat
     3541 cgctcgagct gtcgcgtcgg aactcacaaa gatgagtgtc gacgagaagg ttgtggcggc
     3601 caatctgaaa cgattctcgg atgaagtgca cgctcagcgt gaagcggtcc tgcaggcaat
     3661 gagggactgt ggcagcctcg gcgacacagt tgtggcagta gttccgggcg ttgcttacac
     3721 cgtgcggaac ctcggctatg ccgttggggg tatcctgctt cctgacgaag gaagcggcca
     3781 ggcggatgcc aaaagcctca tgcaaatcag ccgattgctc ggaaaaggtg gcgcacggtt
     3841 gctggtgtgc ccggtaggac tccgaaacgc caaagcgggg gaaatcgctg aacagcttgc
     3901 caaagacacg gggacccgtg tcgcgtacac gcgttttctc cagacaggtg gaacaaacag
     3961 gacatttctc ggggatgcag cgtacaacgc gatggtaacc agcaccgttc tggttgacgt
     4021 ccagccagag gctggtggac tcgcgaatca gagcatatat cccaccctgc tggcgatcat
     4081 gggcggggtc tttctcctgc tcatattcct cgtcatacgt cgcatattgt cgcggtaggg
     4141 attgcatggc cgacgtagct gacgacagaa atccggcggt cgaggtccgt gagctgtcgt
     4201 actcgtacca gcgtcctctt gtggaagcgc tgcgcggggt cagcctgacg ctgccaaggg
     4261 gaacgttcac cgccgttatg gggccgaatg gatccggaaa gagcactttt ctccgtctcc
     4321 tgctcggcat actgcggccc acgaggggtg agatccgcgt actcggagag agcccgacgg
     4381 ctccgtcgca acgcgtgcag cgtctcatcg cgtacgttcc gcagtacgat tcgatcaacc
     4441 ctctccttcc tctgcgaagc aaggaggtca tcgcgctggg actctcgtcc cgagtgactg
     4501 atcggcctgc tcgctggatt gacgaacgcg tttcccagat actcgaagac cttgaccttg
     4561 tggagattcg cgaccatcaa tacggccttc tctccggcgg ccaaaaacag cgggtgctgc
     4621 ttgcccgggc gcttgctctc gaaccggagc tactggtcct cgacgaaccg ttttccgcga
     4681 tggacatgac cagccagcac gcgacggcaa agcttctttc aggactcgta cgccagaaag
     4741 ggaagacggt aataactgtc gtacacggga ttcacaccat tgtgcaccac ctcgacaacc
     4801 tggtcctctt gaacagagag atcgtggctg ccgggacgcc ggacgaggtg ctccgccccg
     4861 caaatcttac gcgggcatac ggcactgacg tgccggtagt catctgcccg gaagggttcc
     4921 ggcacccgct ggtggaagac gtccatgcct gattccgtct cctatctcac gtcaccgttc
     4981 gttctccggg ctgcggcagc atttttcttt gtggggatta atgccgcatt gagcggtgta
     5041 tttgcgaatt tccgcaactc cacctttttg gtttccggtg ccagccatgc cgcactcggg
     5101 ggcgcggcgc ttgtgatcgt actggaaagc cgcggcagcg cttttggtat cagtcccgtc
     5161 ttcggcgcgg cagtctttgc cgttctgctg gcacttgcag ccggcagcgc gcgcaatcgc
     5221 ggcagtacgg ctggttccga tacggcaatc ggggtcgggt tcgctctttc catggcgttg
     5281 g
//
LOCUS       DAOTNG010000223         5264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13104_length_5264_cov_3.704358, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000223 DAOTNG010000000
VERSION     DAOTNG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13104_length_5264_cov_3.704358"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="P5179_10480"
     CDS             <1..629
                     /locus_tag="P5179_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS95686.1"
                     /translation="SAVLLLVLQPLFAAWFGSFVFREKVTSSAITAVILAIVGLLIIT
                     FDDLAISPKQLVGDALAAAASLFMVIFMSLGRTLRPRMSLPAYMTMTYGGAGVWSLIL
                     ALLMGTPLLGYPTSSYLWLLGLIFITTGVGHAAFNYVLPHVRLFTVNIATVAEPALSI
                     LGAVLFLGQRLTIYELIGGALLMAALFVGVRDEKQGTAATQIEQVALE"
     gene            764..841
                     /locus_tag="P5179_10485"
     tRNA            764..841
                     /locus_tag="P5179_10485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:799..801,aa:Pro,seq:cgg)
     gene            863..1141
                     /locus_tag="P5179_10490"
     CDS             863..1141
                     /locus_tag="P5179_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRS95687.1"
                     /translation="MSETVRTFVRVNGSVQGVGFRWYALRCAQRTGVAGWVRNMPDGS
                     VEMEVEGDPAAVNAFIEDMRIGPATADVSEVNFHRIPVKGETSFEVKR"
     gene            1138..1653
                     /locus_tag="P5179_10495"
     CDS             1138..1653
                     /locus_tag="P5179_10495"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRS95688.1"
                     /translation="MTIAANLIRNIPDFPKPGILFRDITPLLNNPAAFSQVIDELADA
                     TSRYRFSRIAGIESRGFIFGAPLSLKLGVGFVPIRKPGKLPARVLREEYSLEYGTNAV
                     EMHADALTKGESVLIIDDLLATGGTAGAACRLVERAGGIVAACTFVIELAALEGRKAL
                     EGREVVSLITY"
     gene            1696..2166
                     /locus_tag="P5179_10500"
     CDS             1696..2166
                     /locus_tag="P5179_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95689.1"
                     /translation="MIWKHAARSALLLTLFIGCNVGPDTRYFLARPLSSRGLAWEIYT
                     RGAWRVVELQGIRPPSYVKEGVLAGGNYFLFAGKFMEPNTLEFRAWTVLPPVRRVVSA
                     ESPPDTTGLPWLTGDDFPKLEDYQRFSLLAAGERWPWRGEGVEKPDSLSFLFRR"
     gene            2265..3107
                     /locus_tag="P5179_10505"
     CDS             2265..3107
                     /locus_tag="P5179_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRS95690.1"
                     /translation="MTPEKPANAFTVDVEDWFQVSAFRHHIGRDEWDHCQLRVEANTE
                     RLLEILASHNVRGTFFVLGWVAERVPALVQRIASAGHEVACHGYHHELVYDCYPSQFE
                     ELTHRAVGVLGELLGKPVTGYRAASFSLPRSERQWVYDILGRQGIRYDSSIFPIRRKF
                     YGHPEAPWTPFDVTTPSGTLREYPLPVVKIAGQAFPFGGGGYFRLYPYHLTASCIRRA
                     NREGRPVVFYVHPWEIDPGQPRQNVGLVTKFRHYVNIKREEERLQRLLREFKFQPLPV
                     GAAG"
     gene            3104..3778
                     /locus_tag="P5179_10510"
     CDS             3104..3778
                     /locus_tag="P5179_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS95691.1"
                     /translation="MNQNTTQETVTRHFDVTAEEFDSIYTGEGKSFWGKLLDRSLRKD
                     MYERFTLTVENCSDDPDRTILDVGCGTGRFSIALAQRNARVDGIDPAPTMLAIATRLA
                     SEQGVADKCRFIEADLDNFSAAEKYDYVIGIGLFDYLATPAAALKRMTDLAKKRVIVT
                     FPRRWTWRAPIRKLRLSLRGCPVFFYDRAQVEKLVRDAGGQIEEVRVVGKIYFVMFAP
                     TDSHRK"
     gene            3788..4897
                     /locus_tag="P5179_10515"
     CDS             3788..4897
                     /locus_tag="P5179_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS95692.1"
                     /translation="MIRVLHVINTLAPGGAETLVTSLCQALPREGVEPLVAYLFGDGT
                     LGERLKDHGIGVERLSHGSRPSLFALPRAISLIRSRKIDLVHAHLVYAGIVAKIAARF
                     TGVPVVMTRHWVSDPKQKTFLFRLDDRLTRRYVSTLIAVGEQVRAAILTEKLMPPERV
                     VVLRNAVDVSRFRVKQHEVVEGESFVVGTVGRLEAPKAHGVFLEAIARLRETFPGVRG
                     LIAGDGRLRSQLEETRVRLGLEGVVDFVGAIPAEGIPDFLNSLDVFVLTSNWEGLPMA
                     LIEASASGLPVVGTDVGGVGEVVVDEVTGYLVPPGNPSEIASRVACLLKNTELRATLG
                     VNARHRAETEFDVTRLAHDTAGLYRATLGIAPADS"
     gene            5078..>5264
                     /locus_tag="P5179_10520"
     CDS             5078..>5264
                     /locus_tag="P5179_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95693.1"
                     /translation="MPQDQKVRVVHIITTFSVGGATETALLCAAGLNATGRFDVSILA
                     GPPNSAEGSLLDDAERWG"
BASE COUNT         1019 a         1563 c         1571 g         1111 t
ORIGIN      
        1 cgagcgcggt tctgctgctg gtgctccaac cccttttcgc cgcgtggttc gggtcgtttg
       61 tgttccggga aaaagttacc tcctccgcga ttaccgccgt catactggca atcgtggggc
      121 ttctcatcat cacgtttgat gacctcgcga tttccccgaa gcaactcgta ggagatgccc
      181 ttgcagcggc agcctcgctt ttcatggtca tcttcatgtc gctcggcaga acactccgcc
      241 cgcgtatgag ccttcccgcg tacatgacga tgacctacgg cggagcgggg gtgtggtccc
      301 tgattctggc tcttctcatg ggtactcccc tcctcggcta cccgacgtcg tcgtacctct
      361 ggctgctcgg gctgattttc atcacaaccg gagtgggaca tgcagcgttc aactacgtgt
      421 taccccatgt gcgcctgttc acggtgaaca tcgccacggt cgccgaaccg gcgctttcca
      481 ttctcggagc ggtgctgttt ctcgggcagc gtctgacgat ctacgaactg atcggtggcg
      541 ctctgctgat ggccgcgttg ttcgtcggtg ttcgtgacga aaaacaggga accgcggcga
      601 cgcagatcga acaggttgct ctcgaatgac cggaggtgcg catccgcgta acggccgctg
      661 cccgtggaag ggcaatctcc gcgctgaaag aggtttgcgg ccttgccgtt ttcgggcgca
      721 gccggtatct tttgtaatct tcagaatcga accgttgcaa agtcggggcg tagcgcagtc
      781 cgggtagcgc acctgcttcg ggagcagggg gtcggtggtt caaatccact cgccccgacc
      841 aacttccttt ccttgccttt tcatgtccga aacagtacgc acgttcgtcc gcgtcaacgg
      901 cagcgttcag ggagtcggct ttcgctggta cgcgctccgc tgtgcccagc gcactggcgt
      961 cgccggctgg gtcaggaaca tgccggacgg gagcgtcgag atggaagtgg aaggggaccc
     1021 tgccgccgtg aacgcgttca ttgaggacat gcgcatcgga cccgccaccg ccgacgtgtc
     1081 ggaggtcaat ttccaccgaa tacccgtgaa gggcgaaacg agctttgagg tgaaacgatg
     1141 actatcgcgg cgaacctgat caggaacatc cctgatttcc ccaaacccgg catccttttc
     1201 cgcgacatca cccctttgtt gaacaacccc gctgcgttct cgcaggtaat tgacgaactt
     1261 gcggacgcca cctcccggta ccgtttctcg cgtatcgcag gtattgagtc ccgtgggttc
     1321 atttttggcg cccctctttc gctgaaactg ggggtggggt tcgtgcccat ccggaaaccg
     1381 ggcaagctgc ccgccagagt actgcgtgag gaatacagcc tcgagtacgg aaccaacgcg
     1441 gtagaaatgc acgcagacgc acttacgaaa ggcgaatcag ttctcattat cgacgatctt
     1501 ctcgccacgg ggggaacagc cggcgcggcg tgccgattgg ttgagcgtgc gggcggcatc
     1561 gtagcggcgt gtacgtttgt gatagaactg gcagccctcg aaggcagaaa agcactcgaa
     1621 ggaagggaag tcgtctcgct gatcacgtat taatcatcga ctgattcatt tctccccagt
     1681 gaggtcgcgg ggcacatgat ctggaaacac gctgcacggt cggctctcct cctcacgttg
     1741 tttattggct gcaatgtcgg cccggacacc cggtatttcc tcgcaagacc gttatcctcc
     1801 agggggttgg cgtgggagat ctacacgcgg ggcgcatggc gagtggttga gttgcagggg
     1861 attcgtccgc cttcctacgt caaggagggt gtgctcgccg ggggcaacta tttccttttt
     1921 gccggaaagt tcatggaacc caacacactc gaattccgtg catggaccgt tctgcctcct
     1981 gtccgccgcg tggtttccgc cgaatcccct cctgacacaa ccggactgcc ctggctgacc
     2041 ggggacgatt tcccaaaact tgaggactat cagcggttct cgcttctcgc cgccggcgag
     2101 cggtggccgt ggcgaggaga aggcgtcgag aaacccgatt ccctctcgtt tctgtttcgc
     2161 cgttaggcga gtatgtttgc ttcaggtgaa aaggcgggcc gtggcgagat gatcggaacg
     2221 cctttttcgg accgacaacc agaaacacaa gggatattcc cggcgtgacc cccgaaaaac
     2281 cggcaaatgc cttcaccgtc gatgtggaag actggtttca ggtttccgcg ttccggcacc
     2341 atatcggaag ggacgaatgg gaccactgcc agttgcgagt tgaggccaat actgagcggc
     2401 tgctggaaat tctcgcgagc cacaacgtac gggggacgtt ctttgtgctc gggtgggttg
     2461 cggagcgcgt gccggctctc gtccagagga ttgccagcgc aggacacgaa gttgcctgcc
     2521 atgggtacca ccacgaactc gtgtacgatt gttatccctc ccagttcgag gaactcacgc
     2581 acagagccgt cggagtgctc ggcgaattgc tgggaaaacc ggtgaccggc tacagggccg
     2641 cgagtttctc ccttccccgt tcagagcggc aatgggtgta tgacatactc gggcgtcaag
     2701 gcattcggta cgattccagc attttcccga tccgtcggaa attctacggc catcctgaag
     2761 ccccctggac ccctttcgat gtgacaacgc cgagcggaac actgcgcgaa taccccttgc
     2821 ccgtcgtgaa gatcgccggc caggcattcc ccttcggggg tggagggtac ttccggttgt
     2881 atccctatca tctcacagcc tcttgtatac gcagagccaa tcgtgaaggc cggccggtcg
     2941 tcttctatgt gcatccgtgg gaaatcgacc cgggtcaacc acgccagaac gtcggtctgg
     3001 taacgaaatt ccgtcattac gtaaacatca agcgtgaaga agaacggctg cagagattgc
     3061 tgcgcgagtt caagtttcaa ccacttccag tcggagccgc gggatgaatc agaacactac
     3121 ccaggaaacc gtgaccagac actttgacgt tactgccgag gaattcgatt cgatctacac
     3181 aggcgaaggt aaaagcttct ggggaaagct tttagaccgg tcgctcagaa aagacatgta
     3241 tgagcggttc acgctcaccg tcgaaaactg cagcgatgat cctgaccgaa ccatcctcga
     3301 tgtcggatgc ggaaccgggc gcttttccat tgcgctcgcg caaagaaatg cgcgcgtgga
     3361 tgggatagac cctgcgccga ccatgttggc catcgccacc agactcgcca gcgagcaggg
     3421 cgtggcagac aaatgccgct tcatcgaagc cgacctcgac aatttcagcg ccgcggagaa
     3481 atacgattac gtcatcggaa ttggcctgtt cgactacctc gcaacccctg ccgcagctct
     3541 caaacgcatg accgaccttg caaaaaaacg cgtgatagtg acattccctc gccggtggac
     3601 atggcgtgcg ccaattcgca aactgcggct gtccctcaga ggctgtccgg tgtttttcta
     3661 cgatcgcgcg caagtcgaga agcttgtccg ggacgcggga gggcaaattg aggaagtccg
     3721 tgtggtgggg aagatttact tcgtcatgtt cgcgccgacc gactctcatc gcaaatgacc
     3781 gtccgcgatg atccgtgtcc tccacgtcat caacaccctc gctccgggcg gggcggaaac
     3841 tctcgtcacg agcctgtgcc aggccctccc gcgtgaaggt gtcgagcctc tggtggccta
     3901 tctcttcggg gacggcacac tcggagaacg cctgaaggac catggcatcg gggtggaacg
     3961 cctcagccac ggtagccgac cctccctctt cgcgcttccc cgcgcgatca gcctgattcg
     4021 atcccggaag atcgacctcg tacacgccca tcttgtctac gccggcattg tggcgaagat
     4081 tgccgcccga ttcaccggtg tcccggtcgt gatgacgcgg cactgggtgt ccgaccccaa
     4141 acaaaagacg tttctgttcc ggctggacga ccggttgaca cgccggtacg tgtctacgtt
     4201 gatcgcggtg ggagagcaag tccgcgctgc catacttacg gaaaagttga tgccgccgga
     4261 acgagtagtc gttctcagga atgccgtgga cgtctcacgt ttccgcgtca agcagcacga
     4321 agtcgttgag ggcgagagct ttgttgtggg cacggttggc cgccttgagg ccccgaaggc
     4381 gcatggcgtg ttcctcgagg caatcgcccg ccttcgtgaa acgtttcccg gagtaagggg
     4441 cctgatcgcg ggtgatgggc ggctccgttc ccaactcgaa gagacccgcg tgcggctcgg
     4501 gttggagggg gtggtcgatt tcgtcggggc gattcctgcg gaagggattc cggatttcct
     4561 caattccctg gatgtgttcg tcctcacttc caactgggaa ggactgccca tggcactcat
     4621 agaggcctcg gcttccgggc tcccggttgt cggaaccgat gtgggtggcg ttggcgaagt
     4681 ggtagtggac gaagtcacgg gctatcttgt gccgccgggg aatccctctg aaatcgcctc
     4741 cagagtggcc tgtctgctca aaaacacgga acttcgagcc actctcggcg tcaacgcacg
     4801 gcatcgggcg gaaaccgagt tcgacgtcac gcgccttgcc cacgacaccg ccgggttgta
     4861 cagggccact ctcggcatcg cgccagccga ttcctgaaat catccgatgc accggcatta
     4921 agagcactcc cgacctcacg attgccgcaa caccagcaag acgggtatct tctgacgtgt
     4981 tgcgcgtcat ctgtttgcgg gtgcgcgctc caatattaac gggctcacgg agcaccgagc
     5041 ctgtcctcct tccggtggtt cgtctgcggg gaaagcgatg ccgcaagatc aaaaggtgcg
     5101 ggtagtccat atcatcacca cgttcagcgt cggtggggca actgagacgg cgctgctctg
     5161 cgcggcgggg ctcaatgcga cagggcgttt cgacgtctca atcctcgcgg gcccgccgaa
     5221 cagcgccgag ggaagccttc ttgacgacgc agaacgttgg ggag
//
LOCUS       DAOTNG010000224         5239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13178_length_5239_cov_4.707562, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000224 DAOTNG010000000
VERSION     DAOTNG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13178_length_5239_cov_4.707562"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            654..1328
                     /gene="tsaB"
                     /locus_tag="P5179_10525"
     CDS             654..1328
                     /gene="tsaB"
                     /locus_tag="P5179_10525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRS95694.1"
                     /translation="MTVLGIDSATPGLGVALAGPEGLIAETTIHVGLQHSQTLLPAAE
                     WLLETVGAGTRDIEGVAVTIGPGSFTAVRIGLATAKGICMAGGIPLACVSTLAASAAR
                     HAWVNLPVVPMLDAKRREVYVGLYDVSTGEPVAVEPDTVVSPQDWLEGHKGEALFCGD
                     GALAYRDLITEKLGAKAHFTPSWLPLASAGVVAVLGRQKILKGETIQPDAATPVYLRR
                     PQAVLP"
     gene            1381..1839
                     /gene="rimI"
                     /locus_tag="P5179_10530"
     CDS             1381..1839
                     /gene="rimI"
                     /locus_tag="P5179_10530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRS95695.1"
                     /translation="MSPIIRAVAADDVEDIAHLETICFPDPWSRASISRILETPSSAI
                     CVAEKEPQGILGYAITTSVSDEGELERIGVAPACRRTGVGRSLLRYALGQLVQAGVKV
                     VWLEVRRSNESALRLYEREGFELVRVRKGYYPPREDALIMRKLLVPEPAG"
     gene            complement(1864..2601)
                     /locus_tag="P5179_10535"
     CDS             complement(1864..2601)
                     /locus_tag="P5179_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS95696.1"
                     /translation="MSECAVLLAAGPGTRLRPLTDTIPKCLVPVGGRPMLDWWLELLA
                     RHGVDRALVATNYLSDQVKAFVRRQKHAVEVEVRDEPVLLGTAGAVTAQAEWLEKEES
                     FWVIYVDNLSSADLSAMRSFHREKKGIGTLGVYETPVPSQCGIVELKNGEEANGSGQV
                     LRLVEKPAFPAGNLANGGLYLLDREILDHIPAPPCDFARDVFPAILAQSSSRPLFAWR
                     IRGYHLDMGTLETYQRAEQDVRNGVFP"
     gene            complement(2594..3703)
                     /locus_tag="P5179_10540"
     CDS             complement(2594..3703)
                     /locus_tag="P5179_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95697.1"
                     /translation="MNVTSETRLCLTSTLERCSVTATAPCRVDASGTWDLKALALPYE
                     HIRPSTANIALNMRTSARLEPYKPGWVLVRNDETYETFPADEVPLDSRFGLVFAAVSH
                     CAVDGVSLTLAHEAPRYAGLGGSGTLMVAVLGALKAAIAVARGERGATLSDEQRAELT
                     ALAHHIEDGLHFSNTGMQDQAAAAFGGVHQWEWRYAANPPFARRSLVPPGEERHLSDR
                     MAIAYVGASHDSSDVNAAQIRDFFSGTTRAQWLEINALGRAFADAIRRGEYGVAADAL
                     QREHEIRCSLVPRRITPVGRVLEGIARDFGCGFAVSGAGNGGCVWALGTDPACVQKLR
                     DAWAATLQEVPTGKVLSSEIAMEGLIVDAGRSNDE"
     gene            complement(3725..4501)
                     /locus_tag="P5179_10545"
     CDS             complement(3725..4501)
                     /locus_tag="P5179_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95698.1"
                     /translation="MRGLITVLVLAISLGVAGCRGKPDLAAVYVQQGRWGEAARLYRE
                     HLAQHPDDQLALRSLAALECYRLGQLEECARTAEHLLSLMPIDSDGVAAVAYARTLLA
                     ERATRQNDTARVRVEMMKVGEAYFKAGYWEYMRENFQRSERFLRKAVLVDPNRVDAYL
                     RLGILFWNRHQTDSSLAWFARAERVAPMNEDALVNQIVVLWQANRIEEAKAVLERLNL
                     VRAQLYPDSSFASPVDTSLPPLSLDFRGDVIEQKKHLITF"
     gene            complement(4498..>5239)
                     /locus_tag="P5179_10550"
     CDS             complement(4498..>5239)
                     /locus_tag="P5179_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRS95699.1"
                     /translation="GVFYPSGLPRSRWLDYYAKSFRVLELNSSYYRIPSAASMQRLAE
                     KMPETHGIVVKANRLTTHEYKDEDASGPFRSALEPLREKWLLRGVLAQFPWSFRNTPG
                     NFQYLAEVTGRMNDVQWFVEFRHRSWLVPEVGAFLREHAIGFVSVDEPQLREMMPPVA
                     KFTVPTAYIRFHGRNADSWWAGGGADRYDYRYSDEELKEWVGKVARLSGQVREILIFF
                     NNCKGGSAAINAKTMRELLKTLPRVEVQ"
BASE COUNT         1054 a         1622 c         1525 g         1038 t
ORIGIN      
        1 cagccagaga gcgcatcggt ccgttccggg gcattgaaag gcccctgatc ccgatttcac
       61 gccttcgaca gggtattcac gccatcaaaa cgcgacaaca gaactgcgct cggagcgcgc
      121 catcgccaca ggtacaccaa agcaccgcgg cctggtcccc cggcaaaacc atcattcttt
      181 gcgcgcgtct ccagagccga tacctcgatc cgtcccgtgc cgttttccag aacccgttcc
      241 cgcttgtcgt tgcggtcacg tacttcgctc gtcggttgcc gtgcgttcca attttccgtt
      301 taatttgcga tcagcgctac agtgtctggc cgcgaaaccg agttgcaggc aaatccgcac
      361 ggcaaccgcg ccgaatttgt ttttcgtggc acactgcact cgggtctggc gaccgatcgc
      421 ccgtatctcc acaagtcccc agaatgcgca taggccgcgc ggaggggaaa gaaaaggcta
      481 aaataaaggg tggaacaatc gcgaacacat gctcttgaaa ccacataacc cgtgttcaaa
      541 aataaaggat tttgcgggaa cccgcaagca gatgcgttac gcatgctggc aaacgacaga
      601 aatcttccgg ggtatctcgc gcggattgca aaacggaagg atagagaggg tatatgacag
      661 ttctcggcat cgactcggcg acaccaggtc tcggagtggc actggccggg ccggaagggc
      721 ttatcgcgga aacaaccatc catgtcggac tccagcactc acagacactc ctgcctgcgg
      781 cagaatggtt gctcgagact gtgggggcgg gcacccgtga tatcgagggt gtggcagtga
      841 cgatcggccc cggctctttt accgctgtgc ggatcggcct tgccacggcg aagggaatct
      901 gcatggcggg cggaataccg cttgcgtgcg tttccaccct ggccgcgtcc gcagcgcggc
      961 atgcctgggt gaatctgccc gttgttccga tgctcgatgc caagcgtcgc gaagtatacg
     1021 tcgggctcta cgatgtgtct acgggagaac cggttgcggt cgaaccggat actgtggtgt
     1081 ccccccaaga ctggctggaa ggccacaagg gggaggcact gttctgcggt gacggcgcgc
     1141 tggcataccg tgatctaatt accgaaaaac ttggcgcgaa ggcacatttc acgccgtcgt
     1201 ggttaccgct tgcttccgcc ggagtggtgg ctgttctggg acgacaaaag atactgaagg
     1261 gcgagaccat ccagccggat gcggccacac ctgtgtatct gcggcgtccc caagccgtcc
     1321 tcccgtaacg cagccgggaa ctgcggtgcg aattcctaac cgttgcgagc gagcgcgaga
     1381 atgtcaccaa ttattcgggc cgtggctgct gatgatgttg aagacatcgc ccatctggag
     1441 acaatctgtt tccctgatcc ctggtcaaga gccagcattt cgaggatctt ggagaccccg
     1501 tcgagcgcga tctgtgttgc cgagaaagag ccgcaaggta tcctggggta cgcgataaca
     1561 accagcgtat cggacgaggg tgagctcgag cgcatcgggg ttgcgccagc ttgcagacgc
     1621 accggcgtgg gtagatcact tctccgctac gctctgggcc aacttgttca ggcaggcgtg
     1681 aaagtcgtgt ggctggaggt gaggcgttcc aacgaaagcg cgttgaggtt gtacgaacga
     1741 gaagggtttg aactggtacg ggttcgcaag gggtattacc cgccccgtga agatgcgctc
     1801 atcatgagga agcttcttgt tcctgaaccc gccgggtgat gatgccggaa cgcgcaccgg
     1861 agcctacgga aacacgccgt ttcttacgtc ctgttctgct cgttggtacg tctccagcgt
     1921 tcccatgtcg aggtgatatc ctcggatccg ccacgcaaaa agaggcctcg aagaagactg
     1981 cgcaaggatc gcagggaaca catcccttgc aaaatcgcag ggtggggcag ggatatggtc
     2041 caggatttcg cggtcgagca ggtacagacc accgtttgca aggttccccg cggggaaggc
     2101 cggtttctcc accaggcgca gaacctgacc ggatccattt gcttcttcgc cgttcttcag
     2161 ttccacgatg ccgcactggg agggaaccgg tgtttcatac acgcccagag ttccgatgcc
     2221 cttcttctcc cggtgaaacg acctcatggc ggaaagatcg gcgctggaga ggttgtccac
     2281 gtaaatgacc cagaacgact cctccttttc caaccattcc gcctgcgccg taactgcacc
     2341 ggcggttccg agaagaaccg gctcatcgcg cacctccacc tcaacggcgt gtttctgccg
     2401 gcggacgaac gctttcacct ggtccgacaa atagttggtt gcgacgagag cgcgatcaac
     2461 gccgtgccgc gcgagaagtt ccagccacca gtccagcatc ggtctcccgc cgaccgggac
     2521 gagacacttc ggaatcgtgt cggtcaacgg acggaggcgg gtgcccggcc ccgccgcaag
     2581 caggacggcg cattcactca tcgtttgacc tgcctgcgtc cacaataagc ccttccattg
     2641 ctatttcgga ggaaagcact ttcccggtag gcacctcctg gagtgtggct gcccacgcgt
     2701 cccgcagctt ctgaacgcac gctgggtccg ttcccaacgc ccagacacaa ccgccattgc
     2761 cggcgcccga caccgcaaag ccgcatccga aatcgcgagc gataccttcc aggacgcgtc
     2821 cgaccggtgt gatgcggcga ggcacgaggc tgcaccgaat ctcgtgttcc cgctggagtg
     2881 cgtccgccgc aacgccgtac tcaccccggc gtatggcgtc agcgaaagct ctccccagcg
     2941 cgttgatttc cagccactgc gcccgcgtgg ttcccgaaaa gaagtcgcgg atctgggcgg
     3001 cattgacgtc gctggaatcg tgacttgctc ccacgtatgc tatcgccatc cggtcggaaa
     3061 ggtggcgttc ctcccccggc ggcacgaggg acctgcgagc gaagggggga ttcgccgcat
     3121 accgccactc ccactggtgc acgccgccga aagcggccgc cgcctggtcc tgcatacctg
     3181 tgttgctgaa atggagtccg tcctcaatgt ggtgcgccag ggccgtcaat tcggcccgct
     3241 gttcatcaga cagggtggca ccgcgttcgc cacgagcaac cgcaatcgcc gctttgagag
     3301 cacccagcac cgccaccatg agagtacctg aacctccgag gccggcgtaa cgcggcgcct
     3361 cgtgggcaag cgttaacgag accccgtcca ccgcgcagtg gctcactgcg gcaaatacca
     3421 gcccgaagcg ggaatccaga ggcacctcat cagccgggaa cgtttcgtaa gtctcgtcgt
     3481 ttctcacaag aacccagccc ggtttgtacg gttcaaggcg ggctgatgtg cgcatattca
     3541 gggcgatgtt cgcggtggag ggccgaatgt gttcatacgg tagtgcgagc gctttgagat
     3601 cccacgttcc gctcgcgtca acccggcagg gagccgtggc cgtgacggaa cagcgttcga
     3661 gcgtagatgt cagacacagt cgcgtttcgc ttgtgacgtt cacaggaatc ctgtcctttc
     3721 cctgtcagaa cgtgatcagg tgttttttct gttcgatcac atcgccccgg aaatcgagtg
     3781 aaagaggcgg caatgatgta tcaactggcg aggcgaaact gctgtccggg tagagctgtg
     3841 ccctgacgag attcagtcgc tccaacacgg cctttgcttc ttcaatcctg ttggcctgcc
     3901 acagaaccac gatctggttg accagtgcgt cctcattcat cggcgcaaca cgttccgcgc
     3961 gggcgaacca ggccagcgat gagtccgtct ggtgcctgtt ccagaacaga atccccagcc
     4021 gcaggtaggc gtctacgcgg ttgggatcca cgagaacagc cttccgaaga aagcgctccg
     4081 atctctggaa gttttcgcgc atgtattccc agtagccagc tttgaagtag gcttcgccca
     4141 ccttcatcat ttccacccgc acccgtgcgg tatcgttctg tcgggtggcc cgttcggcca
     4201 gaagggtccg cgcgtacgca accgcggcaa ccccgtcgct gtcgatcggc atgagggaga
     4261 ggagatgctc ggcggttctg gcacattcct cgagttggcc gaggcggtaa cattccagcg
     4321 ccgccagcga gcgaagtgca agctggtcat cgggatgctg ggccagatgt tcccgataca
     4381 atcgcgcggc ttcgccccac ctgccctgct ggacgtagac ggccgcgagg tctggcttcc
     4441 ccctgcaccc cgccactccc agggatatcg ccagcaccag aaccgtgatc agccccctca
     4501 ttgcacctcc acccggggta gtgtcttcaa aagctcgcgc attgttttcg cattgatcgc
     4561 cgcggaaccg cctttgcagt tgttgaagaa aatgaggatt tcgcgcactt gtccggacag
     4621 ccgtgctacc ttcccaaccc attccttgag ttcctcgtcg ctgtaccgat agtcgtaacg
     4681 atctgccccg ccaccggccc accatgagtc cgcgttgcgt ccgtgaaaac gaatgtacgc
     4741 tgtcggcacc gtgaacttgg caaccggggg catcatttcg cgcaactgtg gttcgtccac
     4801 cgacacaaat ccaatggcgt gctcccgcag gaatgcgcct acctccggta ccagccagct
     4861 ccggtgacga aactcgacaa accactggac atcgttcatg cgacccgtga cttcagccaa
     4921 atactggaaa ttccccggtg tattcctgaa actccacggg aactgcgcca gcacaccccg
     4981 cagaagccac ttctcacgca gcggttcaag ggcgctccgg aagggaccgc tcgcgtcttc
     5041 gtccttgtat tcatgggtgg tcagccggtt ggctttcacg acgattccat gggtttccgg
     5101 cattttctca gccagacgtt gcatcgaggc tgctgatggg atgcggtagt acgaggaatt
     5161 cagttccaga acccggaagg acttcgcgta gtagtccaac cagcgcgacc ttggcaaccc
     5221 ggaaggatag aacactccc
//
LOCUS       DAOTNG010000225         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13275_length_5208_cov_5.536969, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000225 DAOTNG010000000
VERSION     DAOTNG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_5208_cov_5.536969"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="P5179_10555"
     CDS             <1..389
                     /locus_tag="P5179_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRS95700.1"
                     /translation="GVGALAAAVAGYYRGLFGDSRPVMTVVEPDRAACLYESARQGTG
                     QARSVGGDLVRVTANGKGDIERISIDPSLLKPEEKEALEELCSAAVNQAVARARELAQ
                     AEMARLMGGLDLPPGLLDPSLLNGRP"
     gene            394..984
                     /gene="recR"
                     /locus_tag="P5179_10560"
     CDS             394..984
                     /gene="recR"
                     /locus_tag="P5179_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRS95701.1"
                     /translation="MDAVLDNAVRQFMRLPGVGRRTAQRYVYHLLRQGGEQTLALASA
                     VEALHTKIRLCSVCCSLGESDPCGICADSRRDASLICVVGETSEIAVIEGTGRYRGRY
                     HVLGGLLNPAAGIGPDKLTFPALLRRITSEVAEVIVALDASVEGDTTALYISRLLKPT
                     GVRVTTLARGLPAGGSLDHADKVTIGLSLDGRVQLT"
     gene            1019..1606
                     /gene="pgsA"
                     /locus_tag="P5179_10565"
     CDS             1019..1606
                     /gene="pgsA"
                     /locus_tag="P5179_10565"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRS95702.1"
                     /translation="MAKIREFGESANLITLARIVLTPLFVWWFTSADWRLKVLGFVVF
                     VAAAITDWWDGHHARKHKTVSNLGKFLDPLADKLLTIAAMLTLAYEHLVQLWMILVIF
                     ARDFWLTWLRVRAANAGKAFVTSRLAKFKTAVQMTAIITIIGLWCIYSIASTYQMWPG
                     IVPEYWLVALFNVMIAATMLLALVSGFQYLRRSTG"
     gene            1616..2122
                     /locus_tag="P5179_10570"
     CDS             1616..2122
                     /locus_tag="P5179_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRS95703.1"
                     /translation="MIIRREYDTRGVNAFQSISTKLIATVAGAGYSPVAPGTAGTLVG
                     CLVLFILEPDFSSVLFLVAIAVVYAIGVLVSSWAERVWEKSDPGQVCIDELAGLLVSL
                     AYVPAKDPALLLVAAFLLFRMFDIIKPPPARAAENARSGWGIMNDDMIAGLYTNAALQ
                     IISRLGWL"
     gene            2119..3444
                     /locus_tag="P5179_10575"
     CDS             2119..3444
                     /locus_tag="P5179_10575"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRS95704.1"
                     /translation="MKRNTSLQTGEPPRAEIITVGNEVLSGRTVNTNAAFVASELERV
                     GVSVAWVTTVGDVSAHIRDAFLRAWERANVVIVTGGLGPTHDDVTRSVFCEVFTRGLR
                     RDEAVLQNIRSLFAARGRPVTPRNEEQALVPEGTEVLQNRWGTAPGIHWTENGKHWFL
                     LPGVPREMQNLLTHEVLPRLRGLPGARNVVRRELHVVGVPESYLMDRIADITGIEQVA
                     SLPDSKGEVCLRVTVASESYDEAHARCARIVSDLEARLGDAIYGYDGDTLEAVVGRLL
                     AERNMTIAVAESCTGGLVTSRLTDVPGSSRYLVSAVVAYANAAKTDFLGVPLSTLTAH
                     GAVSAETAAAMASGIRNRSQTNLGVAVTGIAGPGGGSPEKPVGLVYLAVADHEGVEVV
                     RELFGDDRLLTKARAAEAALALVWKRLLRKMGNTVTHVNPKDREGNRAT"
     gene            3441..4145
                     /locus_tag="P5179_10580"
     CDS             3441..4145
                     /locus_tag="P5179_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRS95705.1"
                     /translation="MNVLIVTAHPDDMEILCAGTAVRYALRGDSVTICHACTGSLGHM
                     VIPPEELVPIRWEEAEKAAKIAGLRHETLGLPDLQVTERDEVVCLLASLIRDVRPDVI
                     ITHDPNDYMPDHSDLAHLVLKSSFVATLPHHEHVNGECFAVVSPVFFMDTVMGVGFVP
                     EEYVDITDVFDTKKAMLACHASQVDWLRDHDGIDVIENMRVCAQYRGLQCGKKYAEAF
                     RVNRTWPRVTTERLLP"
     gene            4173..4739
                     /locus_tag="P5179_10585"
     CDS             4173..4739
                     /locus_tag="P5179_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRS95706.1"
                     /translation="MGELLPTRDEAWALLCEWTASPGLRAHALAVEAAMRFYARNSGE
                     SEEYWGNVGLLHDFDYERYPDAADHPLKGAEELRRRGYDESFVRAVLSHADYLGLARN
                     TNAEKTLFAVDELCGLIMATAMVMPGRSLAEVTAEAVLKKMKSKGFARKVNREDIVRG
                     TEGLGIPLETHVSNVVRALQGIAATLGL"
     gene            4763..>5208
                     /locus_tag="P5179_10590"
     CDS             4763..>5208
                     /locus_tag="P5179_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRS95707.1"
                     /translation="MATPVILPQMSDTMTEGTLVRWLKAEGDEVEAGDPIAEVETDKA
                     VMELEAPVRGTLLRVFVSPDTVVSCGTRVALIGAPGEEGEDEPPAAPPLTESRVHASP
                     AARKLASRSNIDIRRVPGSGPGGRVVLRDVAAAAAKVLKDPTPSSW"
BASE COUNT         1043 a         1461 c         1673 g         1031 t
ORIGIN      
        1 ccggggtggg ggcgctggcc gccgccgtgg ccggttatta ccgcgggttg tttggcgatt
       61 cgcgcccggt catgacggtg gtggagccgg accgcgcggc ctgcctctat gaatcggcgc
      121 gccaaggcac gggccaagcc cgctccgtcg gcggcgatct ggtcagagtc accgcaaacg
      181 gcaagggaga catcgaacgc atttccattg acccttctct cctgaagccg gaggaaaaag
      241 aagccctcga ggagttgtgt tcagccgcgg tgaatcaggc agttgcccgc gcaagagaac
      301 tggcgcaggc tgaaatggcc agactgatgg gcggcctgga ccttccgccg gggttgctgg
      361 acccgtcgtt actgaacggg aggccgtaac caggtggatg cagtccttga caatgcggtg
      421 cggcaattca tgcgtttgcc tggagtgggg aggcgaacgg cacagcgtta cgtgtaccat
      481 cttcttcggc agggcggcga acagacactg gcgctcgcgt ctgccgtaga ggcactgcac
      541 acaaaaataa ggctttgcag cgtgtgctgt tcgctcgggg aaagcgatcc ctgcggcatc
      601 tgcgcggatt cccggcggga cgcttccctg atctgtgttg tgggagaaac cagcgaaatc
      661 gcggtgatag aagggactgg cagataccgc ggccgttacc acgttctcgg cggtttgctc
      721 aacccggcgg caggaattgg accggataag ctgacgttcc ccgccctgct ccggaggatc
      781 acttcggagg tggccgaagt catcgtggca ctggacgcca gcgttgaagg tgacaccacc
      841 gcactttaca tttcgcggct tctcaaaccc accggcgtgc gcgtcacgac gctcgcgcgc
      901 ggattgcccg ccggcggctc ccttgaccat gcggacaagg ttaccatcgg gttgtcgctc
      961 gatggacggg ttcaactcac gtaggacgag cttgcaatag ctcgctttgc agggttttat
     1021 ggcgaaaatc agagaattcg gcgaatctgc caacctgatc actctggccc gaatcgttct
     1081 tactccgctg ttcgtctggt ggttcacttc ggccgactgg cggctgaagg ttctgggatt
     1141 cgtcgtgttt gttgccgccg caatcacgga ttggtgggac gggcaccatg ccagaaaaca
     1201 caagaccgtc tcgaatcttg gcaagttcct cgatcccctc gcggacaaac tgctcaccat
     1261 cgccgcgatg ctgacgcttg cttacgagca cctcgtacag ctctggatga ttctggtaat
     1321 tttcgcaagg gatttctggt tgacctggct gcgtgtgcgt gcagcgaacg cgggaaaagc
     1381 attcgtaacg tcccgacttg cgaaattcaa aactgcggtg cagatgaccg ccatcatcac
     1441 gattatcggg ctttggtgca tctacagcat tgccagcacg tatcagatgt ggcccgggat
     1501 cgttcccgaa tactggcttg tcgcgctgtt caacgtgatg atcgccgcga cgatgttgct
     1561 ggcacttgta tccggtttcc agtatctccg tcggagcaca ggttaggagc cgaggatgat
     1621 aatccggcgg gagtacgaca cgcgaggcgt gaacgccttt cagtcaatct ccacgaagct
     1681 gatcgccacg gttgccgggg ccggttactc cccggttgca ccgggcaccg cagggaccct
     1741 tgtcggatgc ctggtgctgt tcattctcga gcctgatttt tcctctgtcc tttttctcgt
     1801 tgccattgcc gttgtgtacg cgatcggcgt tctcgtcagc tcttgggccg aacgcgtatg
     1861 ggaaaaaagc gatcccggcc aggtatgcat tgacgaactg gccgggttgc tggtatccct
     1921 cgcgtacgtc cccgccaaag accccgctct tctcctcgtc gcggcgtttc ttctgtttcg
     1981 catgtttgat atcataaagc cgccgcccgc acgcgcggcc gaaaacgcac ggtccgggtg
     2041 ggggatcatg aacgacgaca tgatcgcagg gctctacacg aacgcagcgc tgcaaatcat
     2101 cagtcgactg ggctggttat gaaacggaac acatcgctgc agaccggcga gccgcccaga
     2161 gccgagatta tcacggtagg caacgaggtt ctctcggggc gcacagtgaa caccaatgcc
     2221 gcttttgtcg catcagaatt ggagcgggtg ggtgtctcgg tggcatgggt taccacggtg
     2281 ggagatgttt ccgcgcacat acgcgacgcc ttcctgaggg catgggagcg cgcgaatgtt
     2341 gtgattgtaa ccgggggact cggtccgacg cacgatgacg tgactcgttc ggtgttctgt
     2401 gaggtcttca cgcgcggctt gcgacgggat gaagcggttc tgcagaacat ccgcagcctg
     2461 tttgctgcgc gcggcaggcc ggttactccc aggaatgaag agcaggccct tgttccggaa
     2521 ggaactgagg tgcttcagaa tcggtggggc actgcgcccg gaatccattg gacggagaat
     2581 ggaaaacact ggttcctgtt gccgggtgtt ccgcgcgaaa tgcagaacct tctgacgcac
     2641 gaggtgctcc ccagactcag aggcttaccg ggcgcaagga acgtggtgcg gcgggagctt
     2701 cacgtggtcg gcgtgcccga atcctatttg atggaccgga ttgcagatat tactggaatc
     2761 gaacaggttg catcgcttcc cgactcgaag ggcgaagtat gtctccgcgt caccgtagcc
     2821 tccgagagtt acgacgaagc ccacgcccga tgtgcacgta tcgtctctga tcttgaggcc
     2881 aggctcgggg acgcgattta cgggtatgac ggcgacaccc tggaagccgt ggtaggcagg
     2941 ttgctcgccg aacgaaacat gaccatcgca gtggcagaat cgtgcacagg tggcctggta
     3001 acatctcgct tgacggacgt acccggaagt tccagatacc ttgtttcggc agtagtggca
     3061 tatgcgaatg ccgcgaaaac ggacttcctg ggagtgccgc tgtcaacact taccgcgcac
     3121 ggcgcggtaa gtgcagaaac ggcggccgcc atggcctccg ggatacggaa tcgatcgcag
     3181 acaaatctgg gagttgcagt aaccgggatc gccggaccgg gcggaggttc tccagaaaag
     3241 ccggtcgggc tggtatacct ggcagtggcg gaccacgagg gagtcgaggt ggtgcgcgaa
     3301 ctgttcggcg acgaccgcct gctcaccaaa gcacgagcag cggaagcggc gctggcgctc
     3361 gtgtggaagc ggttgctccg caagatggga aataccgtca cgcatgtgaa tccgaaagac
     3421 cgggagggga acagggcgac atgaatgtcc ttatagtgac cgcacacccc gacgacatgg
     3481 agatcctgtg cgccggaacc gccgtccgct atgcgttgcg gggagacagc gtgacgatct
     3541 gccacgcgtg tacgggttct ctcggacaca tggtaattcc gccagaggag ttggtgccca
     3601 tacgctggga agaagctgag aaagctgcga aaatcgcggg gctgcggcac gaaacgctcg
     3661 gattgcctga tctccaggtc acagaaagag acgaggttgt atgcctgctc gcatcactga
     3721 tccgcgacgt gcgcccggat gtgattatca cacacgatcc aaacgactac atgccggacc
     3781 acagcgatct ggctcacctc gtgctgaaaa gcagcttcgt ggcgacactc ccccatcacg
     3841 aacacgtcaa tggcgagtgc ttcgcggtgg tatccccggt ttttttcatg gacaccgtga
     3901 tgggagtcgg ttttgtcccc gaggaatacg tggacatcac cgacgtcttc gacacgaaga
     3961 aggccatgct ggcctgccat gccagtcagg ttgactggct ccgagaccac gacggaatcg
     4021 atgtaattga aaacatgcgg gtctgtgccc agtaccgcgg gcttcaatgc gggaaaaagt
     4081 atgcggaagc gttccgcgtg aatcgtacgt ggcctcgggt gacaacagaa aggcttttgc
     4141 cgtaacaccg ttccgggaaa gcgagggggt atatgggtga attgcttccc acgagggacg
     4201 aggcatgggc tctcctctgc gaatggaccg cgtctccggg cctgcgcgcc catgcgctgg
     4261 ccgtggaggc cgccatgcgt ttctacgccc ggaattccgg ggaatcggaa gagtactggg
     4321 gcaacgtcgg gctgcttcac gacttcgact acgagcgcta cccggatgcg gcggatcatc
     4381 cgctgaaggg cgcggaagaa ctgcgccgtc gcgggtacga cgagtcgttc gtgcgcgccg
     4441 ttctgagcca tgcggactac ctcgggctgg cccggaacac gaacgcggaa aagacacttt
     4501 tcgccgtgga cgaattgtgc ggtctgataa tggcgacggc gatggttatg ccgggtcgct
     4561 cactcgcaga agttaccgcg gaagcagtgc tcaaaaagat gaagtccaaa gggtttgcgc
     4621 ggaaggtgaa cagggaagac atcgtccgcg gaaccgaggg actgggtatc cccttggaaa
     4681 cgcacgtatc caacgtcgtc cgggcactgc agggaatcgc ggcgacgctc ggtttgtaac
     4741 ttcgggcggg aaaggcatca ccatggcaac acctgtcatt ttgccgcaga tgagcgatac
     4801 gatgacagag ggcacgctcg tacggtggct caaggctgaa ggggatgagg ttgaggcggg
     4861 tgatcctatc gccgaggtgg aaactgacaa ggcggtcatg gaacttgaag cgccggtacg
     4921 gggaacgctt ctgagggttt tcgtctcgcc ggatacggtg gtctcctgcg ggacgcgcgt
     4981 cgccttgatt ggagctccgg gggaggaagg agaagacgag ccccctgccg ctccgccttt
     5041 gaccgaatcg cgagtgcacg cttctcccgc cgcacggaaa cttgcctccc gcagcaacat
     5101 agatatccgt cgggtgcctg gttccggacc aggaggacgc gtcgtcctcc gagacgtggc
     5161 cgccgcggcg gcgaaagtcc tcaaggaccc cactccgagc tcgtggga
//
LOCUS       DAOTNG010000226         5178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13347_length_5178_cov_6.051728, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000226 DAOTNG010000000
VERSION     DAOTNG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13347_length_5178_cov_6.051728"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /gene="leuS"
                     /locus_tag="P5179_10595"
     CDS             <1..1510
                     /gene="leuS"
                     /locus_tag="P5179_10595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRS95708.1"
                     /translation="ANGNRIPVWISDYVLMGYGTGAIMAVPAHDERDFEFARTFNLPV
                     VEVVYHHETVHDANGRIQAAYTGEGTMIQSSGFDGLDSRTGGRQIVEMLARQGKAEAT
                     VSYRLRDWLFSRQRYWGEPIPIVYCEKCGEQPVPEEQLPVLLPEVESYEPTGTGESPL
                     AGITEWVNTVCPVCGGPAKRETDTMPQWAGSSWYFLRYASPDEKAAAFDREKVNKWLP
                     VDMYVGGVEHAILHLLYARFFTKVLFDEGAISFDEPFTKLFNQGMICRKSEKTGRVEK
                     MSKSKGNIVSPDGLVEKYGTDAVRMYELFVGPPEQDAEWNDNGIEGISRFLHRAWGLI
                     TSNLEPSANPERLLRARHRLVKQVTEGLESFKFNTTISRMMEFVNFALDPEGGNGKLD
                     AGTRDAFIVLLAPFAPHMAEELWKLAGKQNSVFDTTVWPSFDEGLASAEKMTIAVQVN
                     GKIRATVELDADSSDETARAEALAQPNVQRYLAGKEPRKIIVVPGRLVSIVV"
     gene            1621..2160
                     /locus_tag="P5179_10600"
     CDS             1621..2160
                     /locus_tag="P5179_10600"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRS95709.1"
                     /translation="MPSKIVLTKGVGRHREQLTSFEMALRDAGLAAFNLVPVSSIFPP
                     RCKLVSKRAGLAELHPGQVVFTVMSRAASNEPHRLLAATIGLAIPRDRNKYGYLSEHH
                     SFGQTEEVAGEYAEDLAAEMLATVLGAEFDVNQSWDEKRELWRIQNEIVATRSISQSA
                     MGHKDGLWTSVLAAAVLIP"
     gene            2324..3223
                     /gene="speB"
                     /locus_tag="P5179_10605"
     CDS             2324..3223
                     /gene="speB"
                     /locus_tag="P5179_10605"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRS95710.1"
                     /translation="MNLSQFPRNLQDVPFTFLGLPDPEGGLGEARVCVLPIPYESTTT
                     YRGGTRSGPAAIVDASRYVETFDEEMLWDLADMPILTLPSVSPLLGAAEAMSERIRDV
                     AREVVSRDRLLISLGGEHAITAPLVEAHSEKFGHLTVIHLDAHADLRESYEGSRMSHA
                     SVIKRVAEHTDVLSVGVRSISSEEMDWLRNQERVRVVFAHEIRKDQGLLERVLGELTG
                     NVYLTIDADVLDPGVMPGVGTPEPGGLSYDQVVHVIKRLSVGCSIVGADLVELAPVPG
                     NVVSEFTAARIVAKIVAYTKNPV"
     gene            3237..3713
                     /locus_tag="P5179_10610"
     CDS             3237..3713
                     /locus_tag="P5179_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRS95711.1"
                     /translation="MAKPNDDTGKTSETVGYPPEKLKEFEAWLHQARQRILKGYDDQK
                     AEIDGFKGDTESDAYDDASNIVEMNILMALNDTQKKELSQIGLALLKVKDGTYGICEG
                     CGELIGEQRLKFLPFANLCVKCKDKEERGTVVIQRDTHIVPPDEDFLVLTDSDEDY"
     gene            3793..4107
                     /locus_tag="P5179_10615"
     CDS             3793..4107
                     /locus_tag="P5179_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95712.1"
                     /translation="MAKSRIETFKAFLEKDPGNSLARYSLAMELRKAGRYDEALAGFA
                     DLIERDPQYVPAYFMCGQTAFEAGKVQEAKEVLRAGIDVARNAGETHAAEKMAELLAT
                     LE"
     gene            complement(4183..5175)
                     /locus_tag="P5179_10620"
     CDS             complement(4183..5175)
                     /locus_tag="P5179_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95713.1"
                     /translation="MVRAAIIGYGGAFNMGAHHTREMHATGRMKLVAVCDIDPARTEQ
                     AKKDWPGIQTFNDVDELLSKGNFDLAVVILPHNQHAPVAVKCSNAGKHVIVEKPMCLT
                     VKEANDMINAAKKARRMLTVYHNRRHDGDFLTLKSLVQRGVIGKVFSVEMGGMGWGHP
                     GTWWRSKKDISGGAFYDWGAHFLDWLLQIVPNKIENVTGFFQQDLVWKDVTNEDHVHA
                     VIRFDENVVADVQMSHIARVGRPRWKLLGSKGAIVSEKGGFRVYGEFDGVLAEGFVSD
                     QPGTHPKYYENIAAHILDGAELDVKPEQARRVIAIMETAEISSKSGVSEKMPVD"
BASE COUNT         1161 a         1380 c         1550 g         1087 t
ORIGIN      
        1 cgcaaacgga aatcgcatac cggtctggat atcggactac gtgctgatgg gatacggaac
       61 gggcgcgatc atggcggttc ccgcacacga cgagcgggat ttcgaattcg cgcgcacatt
      121 caaccttccg gttgtcgagg ttgtgtatca ccatgagacc gtacacgatg cgaacggaag
      181 aattcaggct gcgtacactg gcgaagggac aatgatccag tccagcgggt tcgatgggct
      241 cgattcacgg accggaggca ggcagattgt tgagatgctg gcccggcagg gcaaggccga
      301 agcaaccgtg agctaccggt tgcgcgactg gttattctcg cgccaacgat actgggggga
      361 accgatcccg atcgtgtact gcgagaagtg cggtgagcaa ccggtgccgg aagagcagct
      421 cccggttctt ttgccggagg tggagtcgta tgaacctacc ggcacagggg aatcgccttt
      481 ggccggcatc acggagtggg tgaacacggt atgccccgtg tgcggcggcc ctgcaaagcg
      541 ggaaacggat acgatgcccc agtgggcggg ttcttcctgg tacttcctcc gttatgcgag
      601 ccccgacgaa aaggcggctg cattcgaccg cgagaaagtg aacaaatggt tgccggtgga
      661 catgtatgta ggaggggtgg agcacgcaat tctgcacctg ctctatgcgc ggttcttcac
      721 gaaggtcctg tttgatgagg gggccatttc tttcgatgag ccgttcacga agctgttcaa
      781 ccaggggatg atctgccgca agagcgagaa aaccgggcgc gttgaaaaga tgtcgaaatc
      841 caaaggcaac attgtaagcc ccgacggcct tgtggagaaa tatggcaccg atgcggtccg
      901 aatgtacgag ctctttgtgg gtcctccaga acaggatgcg gaatggaatg acaacgggat
      961 agagggtatc agccgcttcc tgcaccgtgc atggggtctg atcacgtcga atctggagcc
     1021 ttcagcaaat ccggaacggt tgcttcgggc gcgccataga cttgtgaaac aggtgacgga
     1081 aggactcgaa tcgttcaaat tcaacaccac cataagccgc atgatggagt tcgtgaattt
     1141 cgcgctcgac ccggaaggcg gcaacgggaa actcgacgcg ggcacgcggg acgcgttcat
     1201 tgtgttgctc gctccattcg caccgcacat ggcggaagag ctctggaaac tcgccggcaa
     1261 gcagaacagc gtgttcgata cgacagtctg gccttccttc gacgaaggtc tggcgtccgc
     1321 tgagaaaatg acgatcgctg tgcaggtgaa cggcaaaatc cgcgcaacgg ttgagctgga
     1381 tgcggactcc tccgacgaaa ccgcacgcgc cgaggccctt gcgcaaccga acgtccagcg
     1441 gtacctcgcc ggaaaggagc cgcggaaaat catcgttgta cccggccgtc tcgtaagcat
     1501 cgttgtatag gaacgtgcag cagcgccgtt cctcgggagc agtgtccggg ctccatcatg
     1561 gtggagcccc ctttttccac gggatatgga ccagtgaaga cacaaacaat caatcccttc
     1621 atgccgagca aaatcgtact aaccaaaggc gtcggacgcc accgcgaaca actgacgagt
     1681 tttgaaatgg cgttgcgcga tgcggggttg gcagcattca atctggtgcc cgtgtccagt
     1741 attttccccc cccgatgcaa actcgtaagc aagcgtgccg gccttgcaga gcttcatccc
     1801 ggacaggttg tcttcacagt gatgagccgc gcggcgagca acgagccgca ccgcttgctt
     1861 gcggccacga tcgggcttgc gattcccagg gatcgcaaca agtatggcta tctttcggaa
     1921 catcacagct tcgggcagac agaggaagtg gcaggtgagt acgccgagga cctcgcggcg
     1981 gagatgctcg caaccgtgct gggtgctgaa tttgatgtga atcaatcctg ggatgaaaag
     2041 cgggaattgt ggcgcattca gaatgaaatc gtcgcaacaa gaagcatcag ccaaagcgcg
     2101 atgggtcaca aagacgggct ctggacctcc gttctcgcgg cagcggtcct gattccctga
     2161 cgaaatcgtc aaaaggacac cgaaaaaatg gagggttcgc cgaatccggc gtgccctccg
     2221 tttgctttcg aacggggaac ttcagtacct tcccgaacgt ttttgcttga acgcccgagc
     2281 catgtgacac ccttgggtgc aactttcacg caggcgcgaa cccatgaacc tgtcccaatt
     2341 tcccaggaat ctgcaggacg tcccgttcac gttccttggt cttcccgatc ccgagggagg
     2401 gctcggggag gcacgggttt gcgtccttcc cattccctac gaaagcacca caacctatcg
     2461 cggagggacg cgttcagggc cggcggcaat tgtggatgcc tcccggtatg tcgagacgtt
     2521 tgacgaggaa atgctttggg acctggcaga tatgccgatt ctgacacttc cgagtgtcag
     2581 tcctcttctc ggggccgcgg aagcaatgag cgaacggatc cgggacgtgg cccgcgaggt
     2641 tgtttcccgg gaccgccttc tgatcagtct gggtggtgag catgcaatca ccgctccatt
     2701 ggtggaagcg cactcggaaa agttcggtca cctgaccgtt attcacctgg atgcccacgc
     2761 ggatctgcgg gagtcgtatg aaggctcgcg gatgagccac gcttcggtta tcaagcgtgt
     2821 tgcggaacat acagacgttc tgagcgtagg agttcgaagc atctcgtctg aggaaatgga
     2881 ttggctcagg aatcaggaga gggttcgcgt agtctttgcc cacgaaattc ggaaggatca
     2941 aggtcttctg gaaagagtcc taggagagct gacgggcaat gtgtatctga ctattgacgc
     3001 ggatgttctc gatcccggtg tgatgcccgg ggtaggaaca cccgaaccgg gcggactttc
     3061 atacgatcag gtggttcatg ttatcaagag actttcggtg ggatgctcga tagtaggtgc
     3121 ggacctggtt gaactggctc ccgtaccggg caatgttgtc tcggagttca cggctgcgcg
     3181 cattgtggcg aagatagtgg cctacacaaa gaaccctgtc tagaggagcg tttgcgatgg
     3241 caaaacccaa tgacgatacc ggcaaaacat ccgaaactgt cggatatccg ccggagaaac
     3301 tgaaggaatt cgaagcgtgg ctccatcagg ccaggcagag gattctgaaa ggctacgacg
     3361 atcagaaagc tgaaattgac ggcttcaaag gggacacgga atcagacgcg tacgatgacg
     3421 cgtcaaatat cgtagaaatg aatatcctga tggctctcaa tgacactcag aagaaggagc
     3481 tctctcagat cgggcttgcg ctcctgaagg tgaaagacgg aacatacggc atctgcgaag
     3541 ggtgtggaga actcatcggc gaacagcggc tgaagttcct tcctttcgcc aacctgtgtg
     3601 tcaagtgcaa agacaaggaa gagcgtggta ctgtggtcat tcagcgagac acgcacatcg
     3661 tgccgcccga cgaggatttt ctggttttga cggactccga cgaggattac tgagcgtgtt
     3721 gtgtcaggct acgatactga taacatttct tatgtaatgt tgaaaaaaag agccgtcggc
     3781 aaaggatcct ccatggcgaa atcccgaatt gaaacgttca aggcattcct ggagaaagat
     3841 cccggcaaca gcctcgcacg gtactcgctg gcgatggagc tccgcaaggc gggtcgctac
     3901 gatgaggcgt tagccggttt cgcggacctg attgagcgcg acccgcagta cgtgccggcg
     3961 tacttcatgt gcggacaaac cgcatttgag gcgggcaagg tgcaggaggc gaaggaagta
     4021 ctgagagcgg gcatcgacgt cgccaggaac gctggcgaaa cgcatgcggc ggagaagatg
     4081 gcagaattgc tggcaactct cgaatagcgg caggaacaat aggggctgaa acaaacgagg
     4141 gcgcacccga tgcgccctcg tttgtttgtg atggcaccac ggtcaatcga ccggcatctt
     4201 ctcgcttact ccgctcttgg aggagatctc tgctgtttcc atgatggcga tcacgcgcct
     4261 tgcctgttca ggtttgacat caagttcggc gccatcgaga atgtgtgccg cgatgttctc
     4321 gtagtacttg gggtgggtgc ctggctggtc acttacgaag ccttccgcga gcacaccgtc
     4381 aaactcacca taaacacgga agccaccctt ctcgctcacg atcgcgcctt tgcttccgag
     4441 cagcttccac cttggtcttc ccacccgggc tatgtgcgac atctgtacgt ccgcaacgac
     4501 attctcgtca aaacgaatta ctgcatggac gtgatcctcg ttggttacgt ctttccacac
     4561 gaggtcctgc tggaaaaagc ccgtgacgtt ttcgatcttg tttggaacaa tctggagcag
     4621 ccagtcgagg aaatgagcgc cccaatcgta gaatgctcct cccgagatgt cctttttgga
     4681 acgccaccag gtgccggggt gtccccaccc cattcctccc atttccacgc tgaatacttt
     4741 gccaatgaca ccgcgctgta cgagactttt cagagtaagg aaatcgccgt catgtcgccg
     4801 gttgtggtac acggtcaaca tccgccgtgc cttcttcgcg gcgttgatca tgtcattggc
     4861 ttccttaacg gtcaggcaca ttggcttctc gacgatcacg tgcttgcccg cgttggagca
     4921 cttcaccgct acgggcgcgt gctggttgtg tggaaggatg acgacggcca ggtcgaagtt
     4981 gcccttggaa agcagctcat caacgtcgtt aaaggtctgg atgccgggcc aatccttttt
     5041 tgcctgctct gtgcgtgcgg ggtcgatgtc acagacggca acaagcttca ttcggccggt
     5101 tgcgtgcatt tcacgggtat ggtgcgctcc catattgaac gcgcccccat agccgatgat
     5161 tgccgctcga acgatttt
//
LOCUS       DAOTNG010000227         5160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13402_length_5160_cov_4.552987, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000227 DAOTNG010000000
VERSION     DAOTNG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13402_length_5160_cov_4.552987"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="P5179_10625"
     CDS             <1..689
                     /locus_tag="P5179_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRS95714.1"
                     /translation="PPASRGAYLGGLHLFNIVDEDAFYQAADVAEAVSKAPEPYRTPM
                     ENALHQVEEQGSLFPIEIALDLLVLHELWLAGRALTGMDRARIFPMFGVWFDVFNVLS
                     AARLRLNFAVPSDEALGYLIHHGQWLRLSHRRALAGLEKFSDLSAALRDTPYSAKLNG
                     AESLFAVEQGMMRAFREVISASLAGYPFHLGGTVAYLLLKQIEITNLTRVYEGKRRDV
                     KTAGWQDVLV"
     gene            704..2662
                     /locus_tag="P5179_10630"
     CDS             704..2662
                     /locus_tag="P5179_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRS95715.1"
                     /translation="MFRPIPMARIGVIVRDDQSTQLTRLLAAEGVLQIESPSTQLGLD
                     TATARSSISRWEALERQVTELAQNLEIRLSSDKSPFEVVVDPKDDLEPCEKRVAEILS
                     QAEQIRGDLAAIATKRRKTLGQIEELLLLSPVNWTLEELRSTQQLAVHIGVLSEVNVR
                     RVEAILGALPHTTVRLPGPATGWDVVIVFSQKTTADQVERALSSANFKPLEIPEDLTG
                     KPGEAEEKLWKLLEELEEQRKTLTQQLRETTTAEMGELCEIWEKARAARTLLAAQQPF
                     GKTRALSYVTGWVPKESSQALTQRIRDTIGAAAYVEVRDPDPHDPAVPVRLKNPSVFR
                     PFEEVVTTYSLPSSKEIDPTVVVAITYLIMFGMMFGDVGQGLVLAGAGFWAAKKAPSV
                     KNLGLILGMCGISSVIFGFIYGSVFGVEMQLNPWINLPLVEHHTSVMASNLAVLGGIS
                     IGFGIIMLSLGIVFNIVNAVRAKEWGIAVFDQYGLTGLAFYWCALIWAVLTALGYAGL
                     GMTALAAGALVALVVLFVREPIIRATERKEHLVEGGVGFFVVQALIDTMDMVIRFLSN
                     TVSFIRVAAFGLTHAALFMAIHTIGFMISGSTHGAGYIITFIVGNIFVVVMEGLIVSI
                     QCLRLQYYEVFGKFFRGGGLPFAPLRAS"
     gene            2703..3053
                     /locus_tag="P5179_10635"
     CDS             2703..3053
                     /locus_tag="P5179_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRS95716.1"
                     /translation="MKTLKVVLGALLMLNLAVILTAAAVVLFPDLALAAEGAPAAAAG
                     SDRGLMMIGAALAVGLGCVGAGLAVAQVGAAALGAIGEKPDMLGKTLIFVGLAEGIAI
                     YGLIISIMIYASAG"
     gene            3079..3390
                     /locus_tag="P5179_10640"
     CDS             3079..3390
                     /locus_tag="P5179_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRS95717.1"
                     /translation="MKVYCIGSEEVVKGLALIGIEGTATTDAQEAERAVDAVLNQPEV
                     GLLVISERLAMPLKEKLTGWAFAHRQPLILEIPDEQGPVSKHSAVLSLVMEAVGLEQS
                     S"
     gene            3387..4001
                     /locus_tag="P5179_10645"
     CDS             3387..4001
                     /locus_tag="P5179_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRS95718.1"
                     /translation="MNNPIDSLREAVIRDAQAQAYEIMQDAIAAGQRLAAEMDARIAQ
                     DRAEALAKAEQKALQEQQRLLASAQGKIQKERLLEREKRIAEVIEAARSALAEYARQP
                     GVGRDLLLKLCVEGATALRSEKVLLVVRPDQRSWVDTPFLLAVQQQSGKRVELSDENL
                     TAIGGVIVTSSDGRERFDNTLDNRLARVIDDVRAMIWTRISHAR"
     gene            3991..>5160
                     /locus_tag="P5179_10650"
     CDS             3991..>5160
                     /locus_tag="P5179_10650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRS95719.1"
                     /translation="MHDEPVGKVSWVSGPVVRTMETRGVHMLDMVEVGDERLIGEIIG
                     LNGDQATLQVYEDTTGLKPGSPVYSTGYPLAAELGPGLVTSIYDGILRPLKALADATG
                     IFIGRGVRAPSLSREILWDFTPVVRNGQEVTPGMIIGTVPETERIEHRVMVPPNVSGI
                     VTSVAAAGKYKVDQEICRVRTPQGDEVPLMMLQRWPVRQKRPVTRRLPADTPLVTGQR
                     VVDCVFPMVKGGAAAIPGGFGTGKTITQHQLAKWSDADLIVYIGCGERGNEMTEVLVE
                     FPKLIDPHSNRPLMERTILIANTSDMPVAAREASIYTGITLAEYYRDMGYHVALMADS
                     TSRWAEALRELSGRLEEMPAEEGFPAYLATRLAEFYERAGMVETLGGSTGSVSVIG"
BASE COUNT         1055 a         1357 c         1655 g         1093 t
ORIGIN      
        1 tcccgccagc ctcacgcggc gcatacctcg gggggcttca tcttttcaac attgtggacg
       61 aagatgcttt ttatcaggca gctgatgttg cggaagctgt ctccaaggcg ccggagccgt
      121 accgaacacc gatggaaaac gcactccatc aggtcgagga gcaagggtct ctcttcccga
      181 tcgaaattgc cctcgacctt ctcgtgctcc acgagctgtg gctcgccggc cgggcattga
      241 cgggaatgga ccgggcgcgg attttcccga tgttcggggt atggtttgac gtgttcaatg
      301 tgctgtccgc cgcgaggttg cggctcaatt tcgcagtgcc gagcgatgaa gcacttgggt
      361 atctcatcca ccacggacag tggttgaggc tttcacaccg gcgagcgctg gccggtctcg
      421 agaaattcag tgatctttct gcggcgttgc gtgatacgcc gtattccgcg aaactcaatg
      481 gcgcggaatc gctttttgca gtggaacagg ggatgatgcg ggcgtttcgc gaggtgatct
      541 cggcaagtct ggcaggatac ccgttccacc tgggcggcac agttgcatat ttgctgctca
      601 aacagatcga gatcacgaat ctgacacgcg tgtacgaagg caagcggcgc gatgtgaaga
      661 ccgccggctg gcaggatgtt ctcgtgtagg aaggggttcc gtcgtgttcc gaccgatccc
      721 gatggcacga atcggcgtga tcgtgcgaga tgatcaatcc acgcagttga ctcgcctgct
      781 tgcggccgaa ggggtgttgc agatcgaatc tccttcgacc caactcgggc tggataccgc
      841 gactgcgcgt tcgtctatat cccgctggga ggccctggaa cgccaggtaa cagaactggc
      901 gcaaaacctg gaaatccgcc tttcatccga caaatcaccg ttcgaagtgg tggtggaccc
      961 gaaagacgat cttgaaccct gcgagaaaag agtggcggaa attctttcgc aggcggaaca
     1021 gatacggggc gatctggcag caattgcgac gaaacgaaga aaaaccctcg ggcagatcga
     1081 agagcttctg cttctttcgc cggtcaattg gacgctggaa gagttgaggt caacccagca
     1141 actggcggtc cacatcggtg ttctcagcga ggtgaacgtt cggcgcgtag aagcgatcct
     1201 cggagctttg ccgcacacca cggtccgatt gccgggtccg gcgacgggat gggacgtggt
     1261 gatcgtcttc tcccagaaaa ctacggcgga ccaggtggaa agggctcttt caagcgcgaa
     1321 cttcaagccg ctggaaattc ccgaggattt gacgggcaaa ccgggcgaag cggaagagaa
     1381 actctggaag ctgctggagg aacttgaaga gcaacgaaaa acgctgacgc aacagctccg
     1441 agaaacgacg actgctgaga tgggggagct gtgtgagatc tgggagaagg cccgcgccgc
     1501 gagaaccctt ctggcggcgc agcagccgtt cggaaaaacg cgagcccttt cgtacgtcac
     1561 cggttgggtg ccgaaagaat ccagtcaggc actgacacaa cgcatccgtg ataccattgg
     1621 cgctgctgca tacgttgaag ttcgtgatcc ggacccacat gaccccgcgg taccggtgag
     1681 gctcaaaaac ccgtccgtgt tcaggccgtt tgaagaggta gtgacaacct actcccttcc
     1741 ttcgtcgaaa gaaatcgatc cgaccgtagt tgtggcgatt acctacctca tcatgttcgg
     1801 catgatgttt ggagacgtgg gacaagggtt ggtactggca ggtgcggggt tctgggccgc
     1861 gaaaaaagcg ccatcggtga agaatctcgg tctcattctt ggcatgtgcg gcatttcttc
     1921 agtgatcttc ggtttcatat acgggagcgt ttttggtgta gaaatgcagc tgaacccgtg
     1981 gatcaatttg ccccttgtgg aacaccacac ttcggtgatg gcgtctaatc tcgctgttct
     2041 cgggggcatt tccatcggtt tcggaatcat catgctctct ctgggaattg ttttcaacat
     2101 cgtcaacgcc gtgcgggcga aggaatgggg gatcgctgtt ttcgaccagt acgggttgac
     2161 cggtctggcg ttttactggt gtgctctcat ctgggccgtt ttgacggctc taggctatgc
     2221 gggtttgggt atgacggcac tcgcagcggg ggcgctcgtg gctcttgtgg tgctgttcgt
     2281 tcgcgagccg attatccgcg caacggagcg gaaagagcac ctggttgaag gtggagtcgg
     2341 cttttttgtc gtccaggcgc taattgacac aatggacatg gtgatccgct tcctcagcaa
     2401 taccgtctcg ttcatccgtg tcgcggcgtt cgggttgacc catgcggcgc ttttcatggc
     2461 gattcacacg ataggtttca tgatctcggg ttctactcac ggagccggtt atatcatcac
     2521 gttcatcgtg ggcaatatct tcgttgtcgt tatggaaggc ctgattgttt cgattcagtg
     2581 cctgcgtttg cagtattacg aggtgttcgg gaagtttttc cggggcgggg gtctgccgtt
     2641 cgcaccgctt cgcgccagtt gaggttcgct catatcactt caatacgagg ggagtgtggt
     2701 tcatgaagac tctgaaggtc gtgctcggtg cactgctgat gctgaacctc gccgtgatac
     2761 tcacggcggc cgcggtggtg ctgttcccag atctcgcgct tgctgcggaa ggcgctcccg
     2821 cagcggcagc agggagtgat cgaggtctca tgatgatcgg cgcggctttg gcggttggtc
     2881 tggggtgcgt gggtgcaggc cttgcggtag cgcaggttgg cgcggccgct ctgggcgcga
     2941 ttggcgaaaa accggacatg ctcggcaaaa cgctgatctt cgtcggtctt gccgaaggaa
     3001 tagcgattta cggtctgatc atctctatca tgatctacgc ttcggcaggt tgatcggtcg
     3061 acgctccgag agggtgtgat gaaagtctat tgcattggca gcgaggaagt ggtaaaaggg
     3121 ctcgcgctga tcggcattga aggcaccgcc acgaccgatg cccaggaagc ggaacgggca
     3181 gtggacgcgg tgctgaatca gccggaggtc gggctcctcg ttatcagtga acggcttgcc
     3241 atgcccctta aggaaaagct gaccgggtgg gcatttgccc atcgccaacc tctcattctc
     3301 gagatacccg acgagcaggg cccggtatca aaacactcgg ctgttctttc cctcgtcatg
     3361 gaagcggtcg gtctggagca gtcgtcatga acaacccaat tgacagcctc cgggaagcag
     3421 tgatccgtga tgctcaggcg caggcatacg agattatgca ggacgccatc gcagcgggtc
     3481 agcgtcttgc cgctgagatg gacgcccgga tcgcacagga ccgtgcagag gcccttgcga
     3541 aggcggagca aaaggcattg caggagcaac agcgtcttct ggcgagcgcg cagggaaaaa
     3601 tccagaaaga acgcctgctc gaacgggaga aacgcatcgc cgaggttatc gaggcggcac
     3661 gaagtgcgct ggcggaatac gccaggcaac cgggcgtcgg ccgggacctg cttctgaaac
     3721 tctgtgtcga aggcgcgacg gcgttgagaa gcgagaaggt tcttcttgtt gttcgcccgg
     3781 accagcgtag ctgggtcgac acgccatttc ttctggccgt gcaacagcaa tcgggaaaaa
     3841 gagtggagct ttcggacgag aatctcacgg caataggcgg agtgatcgtt acgagttcag
     3901 atggtcgcga gaggttcgat aacacactgg acaatcgtct tgcgcgcgtg attgacgacg
     3961 tgcgggcgat gatatggacg aggataagcc atgcacgatg agcctgtcgg caaagtgagc
     4021 tgggtcagtg gacccgttgt ccgcaccatg gagacgcgcg gcgtccacat gttggacatg
     4081 gtggaagtcg gcgacgaacg gctcatcggc gagatcatcg gcctgaatgg tgatcaagct
     4141 acactccagg tgtacgagga cactaccggc ttgaagccgg gatctccggt gtacagcacc
     4201 ggttaccctc ttgctgcgga gctggggccg gggctggtaa ccagcattta cgacggcatt
     4261 ctgcgcccgc tgaaggcact cgctgatgca accgggatat ttatcggccg cggtgttcgc
     4321 gctccttcac tttcgcgtga gatcctgtgg gatttcactc ccgtcgttcg aaacgggcag
     4381 gaggttaccc ccggcatgat catcggaacc gttccggaaa cggaacggat cgagcaccgg
     4441 gtgatggttc ctccgaacgt ttccgggatc gtgacgagcg tagcagcggc gggcaaatac
     4501 aaagtggatc aggagatttg ccgcgtccgc acgccacagg gcgacgaagt gccgttgatg
     4561 atgctgcaac gatggccggt tcgccagaag aggccggtga cccgccggct gccggctgat
     4621 acgccgctcg tgacgggaca acgcgtggtt gactgcgtct tccccatggt caaaggtggc
     4681 gccgccgcaa ttccgggcgg ttttggaacc ggaaagacga tcacgcagca ccagcttgca
     4741 aagtggtcgg acgccgacct catcgtgtat atcggatgtg gcgaacgtgg caacgagatg
     4801 accgaggtgc tggtcgaatt cccgaagctc atcgacccac acagcaaccg gccattgatg
     4861 gagcggacga tcctgatcgc gaacacctcc gacatgccgg tggccgcgcg tgaggcgagc
     4921 atttacaccg ggatcacgct tgcggagtat taccgggaca tgggctatca cgttgcgctc
     4981 atggccgatt caacgtcccg atgggctgaa gcgctgcgtg agctgtccgg gcggctggaa
     5041 gaaatgcctg cagaggaggg gttcccggca tacctggcga caagactggc ggagttctac
     5101 gagcgtgctg gaatggtcga gacgctcggg ggaagcacgg gatctgtgag tgtgatcggt
//
LOCUS       DAOTNG010000228         5152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13427_length_5152_cov_4.768884, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000228 DAOTNG010000000
VERSION     DAOTNG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13427_length_5152_cov_4.768884"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="P5179_10655"
     CDS             complement(<1..472)
                     /locus_tag="P5179_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRS95720.1"
                     /translation="MTSRSRLPRATVSQLERIVGRGFVIVDETLLQLYGETRGSDSIR
                     PLAILLPSTDEQVSEIFRLLGREGLGASVRGGGSGLVWGALPHDAVTVISTARMNTPL
                     RVVAEQMRVTAGAGVRIEEMVAAVTAHGFRLSGGMDMPPFATAGGFVARNGDGYG"
     gene            596..1471
                     /locus_tag="P5179_10660"
     CDS             596..1471
                     /locus_tag="P5179_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95721.1"
                     /translation="MFKLSVFTDEISQDFERAVAVASEYRLDGVEIRSVWDKPPQQLD
                     NRDIREIREILKGTGLKVASIASPFFKCHLGNAEERKEHIQILERCSRLADALECSII
                     RGFTFWRETPVENHWQDILAAFAEPLTLLEKYDKTLAIENEASTMVGTGRRLARFLGD
                     LNTDRVAAMWDPANCIYDDEDREIPFPDGYEAVKHRMRHMHLKDALRDPASNEPRCVP
                     IGEGLVDYRAQFPALVADGYDGFVSLETHWRPTALDEEQLNRPGGAAFSKEGEYATRV
                     CLDNLFRMLADLGLR"
     gene            1632..2879
                     /gene="glyA"
                     /locus_tag="P5179_10665"
     CDS             1632..2879
                     /gene="glyA"
                     /locus_tag="P5179_10665"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRS95722.1"
                     /translation="MSNLRGVDPEVWALIQQEELRQTSKIRLIPSENYVSKAVLEATG
                     SVFTNKYSEGYAGNRYYEGQQNVDVLETLAQNRAKELFGARYVNVQPYSGSPANQAVC
                     HALLEPGDTIMGLDLRHGGHLTHGWKVNFSARYYRSVPYRVNPATGLLDYDALEALAN
                     EHRPKLIICGATAYSRIIDFPRIAEIARSTGAYSLADIAHISGLIVAGEHPSPVGVTD
                     VVSTTTHKSLRGPRGAMIMTNSEELAKKIDRAVFPGLQGGPHNNTTAAIAVALKEAAT
                     PEFREYGRQIVRNAKTLADALAKKGYNLISGGTDNHLILIDLTNKGISGKIAARALDL
                     GGIETNYNTIPDDPRKANDPSGLRIGTPAVTTRGMKEAEMRQIAEWMDLIIRNHDDET
                     LLDSTRNEIASFCEAFPAPGITT"
     gene            complement(2931..3860)
                     /locus_tag="P5179_10670"
     CDS             complement(2931..3860)
                     /locus_tag="P5179_10670"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRS95723.1"
                     /translation="MPIRSRVAPIILLAGIAAAGCGNQVGDEQVREKQGEGASELAVV
                     SPVADVQGGAPGAMMATRGGIQNPVPSPMASMPVDTFRRQIIKTVDMTLEVSKVDSAL
                     AWAERIALEAGGYVGSVSRNEAPPEGARSGMITLKVPAGKTDECLSRLRALGALKNEH
                     IWTEDVTEDYYDTSIRLKNAQAAREQFEALLRQARRVPEIVEVQRELQRVTEEIERMT
                     GHLRRLSNMIAFSTVNLSVTEPVPILHDRTSVWGRLATALRGMVDAFWSTIAWLIRML
                     GVVIPVGLLIGAAWWSVRTYLKARTRGKSRNAG"
     gene            complement(3866..4780)
                     /gene="lipA"
                     /locus_tag="P5179_10675"
     CDS             complement(3866..4780)
                     /gene="lipA"
                     /locus_tag="P5179_10675"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRS95724.1"
                     /translation="MPKPLPDTSEPNGEIEQGKRRLPRWFRVPAPGNERYREIQALLR
                     REHLHTVCEGAHCPNIGECWGIHGTATFMILGNCCTRRCRFCAVEKGTPSPPDISEAD
                     SVARAVQTLGLRHVVVTSVTRDDLPDGGASTFAEVIHRIRDANQDVTIEVLIPDFRGD
                     VESLKVVLEARPDVLNHNLETVRRLYAAARPQSDYERSLSVLRHASKQGCVTKTGIMV
                     GLGERWDELEELFADAARAGCSMMTIGQYLRPSEHHLPIDRFYTPDEFAMLRSSAEKA
                     GVSHVESGPLVRSSYHAAQQVNHATRER"
     gene            complement(4780..>5152)
                     /locus_tag="P5179_10680"
     CDS             complement(4780..>5152)
                     /locus_tag="P5179_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS95725.1"
                     /translation="NLVSISVKQVVENLVLSTAIRSAVVGLAKTLATELGPCGITVNN
                     VCPGYIGTKRLESLWGGAAEKSGVTYDEYCRNQAKAIPLGRIGTPQDVASLVVFLASS
                     RAGYMTGTTIQVDGGLCKGLL"
BASE COUNT         1084 a         1587 c         1437 g         1044 t
ORIGIN      
        1 caccgtaacc gtccccgttt ctcgcgacaa atcctcctgc cgtggcaaac ggaggcatgt
       61 ccatcccgcc agaaagtcgg aatccgtgtg cagtcacggc cgcaaccatt tcctctatcc
      121 gcactcctgc tcccgcagtc accctcatct gctctgcaac aactcgcagg ggtgtgttca
      181 ttcgcgcagt gctgatcact gtaaccgcgt cgtgtggaag cgccccccag actagacctg
      241 agccgccccc gcgcaccgaa gcaccaagcc cctctctccc gagcaggcga aaaatctcag
      301 aaacctgctc atccgtggag ggcagcagga ttgccagtgg acggatggaa tcgctgcctc
      361 tggtttcgcc gtacagttgc agcaacgttt cgtcgacgat cacgaaaccg cgtccgacaa
      421 tccgttcaag ctgcgatacg gttgctcgcg gcaaacgact tcgagacgtc acgggtcttc
      481 gtccaaaagg atgcgcttgt tcatatctcc gcaacccgcg tatacttttt cacaggccgc
      541 gcaacgccct gccgggtaac acaaagcact ttggccacct gaagggatat cctgtatgtt
      601 caagctgtca gtcttcacgg atgagatttc gcaggacttt gaacgagcgg tagccgtcgc
      661 atcggagtac cgtctcgatg gcgtcgagat acggtctgtc tgggacaaac cccctcaaca
      721 actggacaac cgggatatcc gcgagattcg ggaaatcctg aaaggaaccg gcctcaaagt
      781 ggccagcata gcgtccccct ttttcaaatg ccatctgggc aacgccgagg aacgaaaaga
      841 acacattcag attctcgagc ggtgttcccg tctcgctgac gctcttgagt gctcgattat
      901 caggggattc acgttctggc gcgaaacgcc cgtggaaaac cactggcagg acatcctggc
      961 cgcttttgcc gagcctctta cccttcttga aaaatacgac aagaccctgg caattgagaa
     1021 tgaagcaagc acaatggtcg gaactggcag gcggctcgcg agatttctgg gcgatctcaa
     1081 cacggaccgt gttgctgcaa tgtgggaccc cgcaaattgc atctacgacg acgaagacag
     1141 ggaaatcccg ttccccgatg ggtacgaggc ggtgaagcac aggatgcgcc acatgcatct
     1201 caaggacgcg ttgcgggacc ccgcttcgaa cgaaccccgg tgtgtgccaa tcggcgaggg
     1261 acttgttgat taccgcgctc agttccctgc acttgttgcc gatggttacg atgggttcgt
     1321 ctcgcttgaa acccactgga ggccgacagc cctcgatgag gaacaactga accgacccgg
     1381 cggggccgcc ttcagcaagg aaggcgagta cgccacgcgg gtctgcctcg ataacctctt
     1441 ccggatgctt gcggatctgg gcctcagata acatactctg gggcccaccg atacgccgcg
     1501 cccttgagtt tcgccttgcg aagccggtcg cgcgaacggt acatttcagt tcttaccaca
     1561 ggtggatcca ttttcccaga tccaacggca tctggtcccc ggtggtgttt ctgtccccta
     1621 aggagttttc gatgagcaat ctgcgtggtg ttgatcccga agtgtgggca ttgattcagc
     1681 aggaagagct ccgtcaaacg agcaaaatcc ggttgattcc ctcggaaaac tatgtctcca
     1741 aagcagttct tgaggccaca ggttcagtgt tcacgaacaa gtactccgag gggtacgcgg
     1801 ggaaccgcta ttacgaggga cagcaaaacg ttgatgtgct cgaaacactc gcccagaacc
     1861 gcgcgaaaga gctgttcggc gcaagatatg tgaacgttca gccctattct ggatcaccag
     1921 ccaaccaggc cgtatgccac gctctgctcg agccaggcga tacaattatg gggctcgatc
     1981 tccgtcatgg tgggcacctt acgcacggat ggaaggtgaa tttctccgcc cggtattacc
     2041 gttcagtccc gtatcgcgtc aaccccgcaa ccggcctctt ggactacgat gccctggagg
     2101 cgttggcgaa tgaacacagg ccgaagctga tcatctgcgg ggcaacggcg tactcccgca
     2161 tcatagattt cccccggatt gccgaaatag cacgatccac cggagcgtat tcgcttgccg
     2221 acatcgccca tatttccggt ctgatcgtcg ccggggagca tccatcgccc gtaggggtta
     2281 ccgacgtggt ctccacgaca acgcacaaat cgttgcgtgg gccgcgcggg gcaatgatta
     2341 tgaccaattc agaggaactt gccaaaaaaa tcgaccgagc ggtgttcccc ggtctccagg
     2401 gaggcccgca caacaatacg actgctgcca tcgccgtcgc gctgaaagaa gccgccactc
     2461 ccgagtttcg ggaatacggc aggcagatcg tgcgcaacgc gaaaacactc gctgacgcgt
     2521 tggcaaaaaa ggggtacaac ctcatttcag gcggaacaga caaccatctg attctaattg
     2581 acttgacgaa taaggggatt tccggcaaaa tcgccgcacg cgcgctggac ctcggcggga
     2641 tagaaacaaa ctacaacacc atccccgacg acccacggaa agcgaacgac ccttctggcc
     2701 tcagaatcgg aacgccggcc gttacaactc gcggtatgaa ggaagcggaa atgcgtcaga
     2761 ttgcggaatg gatggacctg ataatccgca atcacgatga cgaaacgctg ctcgactcca
     2821 cgcggaacga gatcgcatcg ttctgcgaag cgtttcccgc gcccggcatc actacgtgat
     2881 ggacgcccgc gctgcacaac ggattcacaa ccccgcgggc gggtcctgac tcaacccgcg
     2941 ttgcgtgatt tccctcgtgt gcgcgctttg aggtaggtcc gaaccgacca ccacgctgca
     3001 ccgatcagca atcctaccgg gatcaccacc ccgagcatac gtatcagcca cgcgatggta
     3061 ctccagaacg cgtccaccat tccccgcagg gctgttgcca gcctgcccca cacgcttgtt
     3121 ctgtcgtgaa gtatgggaac tggttcggta acgctcaggt tgaccgttga aaacgcgatc
     3181 atgttcgaga ggcggcgcag atggccggtc attcgttcga tttcctcggt cacgcgttga
     3241 agttcgcgct ggacctcgac gatttccggt accctgcgtg cctggcggag cagcgcctca
     3301 aactgctcgc gggcggcctg cgcatttttc aggcgtattg acgtgtcgta atagtcttcg
     3361 gtgacatcct ccgtccagat gtgctcgttt ttcaatgctc cgagagcgcg gagcctggac
     3421 aggcactcgt ccgtctttcc ggcgggcact ttgagcgtga tcatgccgct gcgggcacct
     3481 tcagggggtg cctcgttcct gcttaccgac cccacataac cgcctgcttc gagcgcgatc
     3541 cgctcagccc atgcgagggc tgagtcaact ttcgagactt ccagtgtcat gtccaccgtc
     3601 ttgatgatct gacggcggaa ggtatcgacg ggcatcgaag ccatcgggct cgggaccgga
     3661 ttttgaatgc ctccgcgagt tgccatcatg gcccccggtg cgccgccctg gacgtccgcg
     3721 actggcgaaa ccacggccaa ctcactggca ccttctccct gcttctcccg cacctgctcg
     3781 tctccgacct gattgccgca tcccgccgcg gcgatgccgg ccagaagaat gatcggggcg
     3841 acacgcgatc tgatcggcat tttgttcacc tttcacgagt ggcgtggttc acctgctggg
     3901 ccgcgtggta tgaacttctg accaacgggc cgctttcgac atggcttaca ccggccttct
     3961 ccgcgctgct tcgaagcatc gcgaactcgt caggggtgta aaaacgatct atgggaaggt
     4021 gatgctcaga cgggcgcaga tactgtccga tcgtcatcat tgaacacccg gcacgcgcgg
     4081 catcagcaaa cagttcttca agctcgtccc atctctcgcc caatcccacc atgataccag
     4141 ttttggtgac acatccctgc ttcgaggcat ggcgaagcac agagagggag cgttcgtaat
     4201 cgctctgcgg ccgcgccgcc gcgtacaacc gtcgcacggt ttccaggttg tgattcagca
     4261 cgtccgggcg cgcttccaga acgaccttga gcgattcaac gtccccacgg aaatcgggaa
     4321 tcagaacctc gatggtgacg tcctggttcg cgtcgcggat gcggtgaatc acctcggcga
     4381 atgtacttgc cccgccgtcc gggagatcgt ccctcgtcac ggaggtgact acaacgtggc
     4441 gaaggccgag ggtctggact gcccgtgcta cgctgtccgc ctcgctgata tccggggggg
     4501 aaggcgttcc tttttctacc gcacagaagc gacaccgacg tgtacagcaa ttgcctagga
     4561 tcatgaacgt ggcagtgcca tgtatacccc agcattcgcc gatgttcgga caatgcgctc
     4621 cctcacagac cgtgtggagg tgctcgcgcc gaagaagagc ctggatctcg cggtaccgtt
     4681 cattcccggg cgccggaaca cggaaccacc gcggcagtcg acgcttcccc tgctctatct
     4741 cgccgttggg ttcggatgtg tctggaaggg gcttcggcat cacaggagac ccttgcaaag
     4801 accgccgtcc acctggattg tggtgcccgt catatacccg gctcttgacg acgcgaggaa
     4861 cacgacgagg gaggcaacgt cctgtggggt cccaatcctg ccgagaggaa tcgctttcgc
     4921 ctgattgcgg cagtactcgt catacgtgac tccggatttt tcagcggctc caccccagag
     4981 ggattccagg cgcttcgtgc cgatatagcc ggggcagacg ttgttgaccg tgataccaca
     5041 gggtccgagt tccgtcgcga gagttttcgc aaggccgaca acggcagaac ggatcgcggt
     5101 cgaaagcacg aggttttcta cgacctgctt cacgctgatc gaaaccaggt tc
//
LOCUS       DAOTNG010000229         5100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13582_length_5100_cov_5.278097, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000229 DAOTNG010000000
VERSION     DAOTNG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13582_length_5100_cov_5.278097"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="P5179_10685"
     CDS             complement(<1..290)
                     /locus_tag="P5179_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95726.1"
                     /translation="MPHTPKFYPFSDVPVSDSCWSLSTGPDGRIYIAVCSEGLGGVSV
                     YIARYNDETDRLDYLVDASKETNDPPDSGRATQCKIHYCFCPSRHDGILYAAT"
     gene            475..960
                     /locus_tag="P5179_10690"
     CDS             475..960
                     /locus_tag="P5179_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS95727.1"
                     /translation="MDFQPGKHYIGVGVGGIITGSDGRVLLARRSNAARNQRGQWENP
                     GGALEFGERFEDAIVREIHEELDIEVVPVGLLRVVNHIIPADHQHWVSPTFVCRHVRG
                     VPHINEPLKCSAAEWFALDDLPEPLTAISRTDLEYYKQVVESGRPLLPVTWCSPELPV
                     S"
     gene            1001..4921
                     /locus_tag="P5179_10695"
     CDS             1001..4921
                     /locus_tag="P5179_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRS95728.1"
                     /translation="MNLFKRFFNKSKREIKRLRPVVAEINAKYELLHDLPDEAVKGKT
                     DEFRKRLADGETLDDLMVEAFAVVKETMRRFTERKMEWDVCGNMTPWFMVPFDVQLVG
                     AIVLHQGKIAEMATGEGKTLVAVLPLYLNALPGKGAHLVTVNDYLARRDASWNGPIYH
                     WLGLSVSVLQDPNANNGIESYKVDWNETQGYHLVPISRREAYACDVTYGRQDQFGFDY
                     LYDNMAWDIADIRHRGFNFAIVDEADQLLIDEARTPLIISGQVPESKSERYVELKPRV
                     EKLVKEQSRIVAELVDDGERLWEAGETYAGAKKLLQAQRGAPKQRKLMRLCESGEVKK
                     AIVRVELDYMRDKRTPELDEPLLYSIDEKNHSAEITDEGRSRLLLTEDKRRFISLMDT
                     VRRIERDENVDDAVKQERLEKEFRAYINETKGRAITSEAGDVVRAIELDEVLTDEQKR
                     VLLKEQFALAVDYASRGFIRFSLTDEAEGPEDDAQADRDAGRVKTVESASNWVKQAIK
                     RLSYPGREIEERISANEYLTSQDKGDLTKALSDAFSTAEREGLSRPSFDDVTAFVTHN
                     EKLDTPAKAELLADAYRAYMEAQTQRLVLPDLAQILHHVEQNDVLSADQKFALVEDMF
                     RVYFDDSRRKFILPDLSENLELIEKSTVFSEDQKRKLIDFQYSSYADAADRIHGMSNL
                     IRAYGLFERDVNYVVQDDKVVIVDEFTGRLQPGRRYSDGLHQALEAKENVTVERDTQT
                     VATITVQNYFRMYDKLAGMTGTAETEEDELWGIYKLEVIVIPTNKPVRRIDLDDVIFR
                     TKREKFNAVVEEVEQLHQQHLPVLIGTRSVETSELLSRMLKRKGIAHSVLNAKYHQIE
                     AEIIAKAGQPDAVTIATNMAGRGTDIKIPRSLIKCDECKLRSGLPESTITADGMTIKD
                     CLEKQPCGLHIIGTERHDARRIDRQLRGRSGRQGDPGASQFFLSLEDDLMRLFGSERI
                     ANIMTRLGAQEGEVIQSPLVTKAIDRNQRTIEANNFEIRKRLLDYDDVMNQQREVIYD
                     RRRFALENDDLTGEIRSLTMNAIDDIITRFAENENDGEGWDFGALNAELSKRFGVEAN
                     LRFGPEETVSDEVIRQRVTNAVLSKYEARCNELRREARRQGAEDEEVFVRRLHKIAVL
                     SAVDDRWREHLTDIEDMKGGIHLRQYGQKDPVVEFKREAYDIFENLIREIDRQTLEIV
                     FKSQLPSEREVEARRRAAAELMATHAEMEGFDAGEGAPVAVAEPEIRTVGGRGARKPV
                     TAARASAPKSAPQQTKAVPRVSRNSPCPCGSGKKYKYCHGKDET"
     gene            4918..>5100
                     /locus_tag="P5179_10700"
     CDS             4918..>5100
                     /locus_tag="P5179_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HRS95729.1"
                     /translation="MTTKTGLSRPQTALTILAAVGFPISLYLIFVVTPLDAELGFTQK
                     IFYFHVPCAWITYLAFF"
BASE COUNT         1222 a         1365 c         1527 g          986 t
ORIGIN      
        1 gttgccgcgt acagaatgcc gtcgtgcctg gaagggcaaa agcagtagtg gattttgcac
       61 tgggtcgctc gcccggaatc gggcgggtcg tttgtctcct tcgaggcgtc cacaaggtag
      121 tcgaggcgat ccgtttcatc attgtaccgg gcgatgtaga cacttacacc gcccagcccc
      181 tcggaacaga ccgcgatgta gatgcgcccg tcgggccctg ttgagaggga ccagcacgag
      241 tctgataccg gcacgtccga aaacgggtaa aacttcggag tgtgaggcat cgctatctcc
      301 ttggagtgtg gtgaggcgca aggcgggagg aggtccccga ctcagcgcga acaatgaaga
      361 ttataggcgc cttcgtctca ataccaaagc ccggaggaac ggcttatgct tgagaacctg
      421 tgggtacggg cgtagattta atgggttgtc atttgttgga cggagaaatg tggcgtggat
      481 tttcaaccgg ggaagcacta cataggcgtg ggagtgggcg ggatcattac gggctctgac
      541 gggcgggttc tgcttgcccg aaggagcaac gccgctcgca atcagagagg tcaatgggag
      601 aaccccgggg gtgcgctgga atttggagag cgttttgaag acgcgatcgt ccgcgaaatt
      661 cacgaagaac ttgacataga ggttgtgccg gtagggcttc tgcgcgttgt caatcacatc
      721 attccggcgg accatcagca ctgggtttcc cctacatttg tctgccggca tgttcgtggt
      781 gtccctcaca tcaacgaacc gctgaaatgc agtgcggcgg aatggttcgc gttggatgat
      841 ctgcctgagc cgctgaccgc gatctcgcgt actgatctcg agtattacaa acaagttgtc
      901 gaatccggtc gcccactgct gccagtaacc tggtgctccc cggagttacc tgtctcatga
      961 tgacgttctc tcgatcgccg atacaaacgg gagtgttgct gtgaatttgt tcaaaaggtt
     1021 tttcaacaag agcaaacgcg aaatcaagcg tcttcggcct gtcgtcgccg agataaacgc
     1081 gaagtacgag ttgcttcatg atctgccgga tgaggcagtc aaaggaaaaa ccgacgaatt
     1141 cagaaagcgc ctggcggacg gcgagaccct ggacgatctc atggtcgagg cgttcgccgt
     1201 tgtcaaagag acgatgcgcc gtttcactga acggaagatg gaatgggatg tctgcgggaa
     1261 catgactccg tggttcatgg tgccgtttga tgttcagctg gtgggtgcca tcgttctgca
     1321 tcaaggcaaa atcgcggaaa tggcgaccgg agaaggcaag actctcgtcg ccgtattgcc
     1381 gctctacctc aacgcgcttc ccggaaaagg tgcgcacctc gtcaccgtga atgactacct
     1441 cgcccggcgc gacgcaagct ggaatggtcc catttaccac tggctcgggc tctcggtttc
     1501 cgttcttcag gatccgaacg cgaacaacgg cattgagagt tacaaagtcg actggaatga
     1561 aacgcagggg taccaccttg tgccgatctc tcgccgggaa gcttacgcct gtgatgtgac
     1621 ctacggcagg caggatcagt tcggatttga ctatctctac gacaatatgg cgtgggatat
     1681 tgccgacatt cgtcatcggg gcttcaattt cgcgatcgtg gacgaggctg accagcttct
     1741 tatcgacgag gcacggacgc cgctcatcat cagcgggcag gtacctgagt ccaaaagcga
     1801 acgttacgtg gaacttaagc ctcgcgtgga gaagctggta aaggaacaga gccggatagt
     1861 cgcggaactg gtcgacgacg gcgagcggct gtgggaagcg ggagaaacgt acgccggcgc
     1921 aaagaagctg ctgcaggctc aacgcggcgc tccgaaacaa cgaaaactca tgcggctctg
     1981 cgagagcggc gaggtgaaaa aggcgatcgt ccgcgtagaa ctcgattaca tgcgcgataa
     2041 gcgcactccc gaactcgacg aaccgttgct ctattcgatc gacgagaaaa atcattcggc
     2101 ggagatcact gacgagggcc gctcccgcct tctgctcacc gaggacaaaa gacgcttcat
     2161 ttcgctcatg gacaccgtcc gccgcatcga acgcgatgag aacgttgacg acgcggtcaa
     2221 acaggagcgc ctcgagaagg aattccgtgc gtacatcaac gagacgaagg ggcgagcgat
     2281 cacgtcagaa gctggcgacg tcgttcgtgc gattgaactc gatgaggtac tcaccgacga
     2341 gcagaagcgc gtgctgctca aggaacagtt tgcactggcc gtggactacg cgtcccgcgg
     2401 gttcattcga ttctcgctga ccgacgaggc cgagggtccg gaagacgatg cgcaagcgga
     2461 ccgcgacgcc ggacgcgtaa aaaccgtcga gtccgcatcg aactgggtaa aacaggccat
     2521 caagcgtctc tcgtaccctg gaagagaaat cgaggaacga atctcggcga atgagtactt
     2581 gacgagccag gacaaagggg acctcaccaa ggctctttcc gatgcattct ccacagcgga
     2641 acgagaaggg ctttcgcggc catctttcga cgacgtgacg gcgttcgtca ctcacaacga
     2701 aaagctggac actcctgcga aagcggagct gctggctgat gcgtatcgcg cgtacatgga
     2761 ggcgcagacc cagagacttg ttctgcccga tcttgcccag atcctgcacc atgttgaaca
     2821 gaatgacgtc ctctcggcgg atcagaaatt cgcgctggtc gaagacatgt ttcgcgtgta
     2881 tttcgacgat tcccggcgca agtttatcct tcccgacctc agcgagaacc ttgaactgat
     2941 cgagaagagc accgttttct cagaggacca gaagaggaag ctcatcgatt tccagtattc
     3001 gagctatgcg gatgcggcag accgcatcca cggaatgtcc aatctcatcc gtgcatacgg
     3061 gctgttcgaa cgcgacgtga actacgtggt tcaggatgac aaggtcgtca tcgtcgacga
     3121 gtttacggga aggcttcaac cggggcgacg gtattccgac ggactgcatc aggctcttga
     3181 agcgaaagaa aacgtcacgg tggaacgtga cacgcagacc gtggccacca ttacggtcca
     3241 gaattacttc cgcatgtacg acaaactcgc gggtatgacg ggtaccgcgg aaaccgaaga
     3301 agatgagttg tggggaatct acaagctgga agtcatcgtc atcccgacga acaaacccgt
     3361 gaggcgaatc gatctggacg acgtgatctt ccgcacgaag cgggagaagt tcaacgccgt
     3421 cgtcgaagag gtagaacaac tgcaccagca gcatctgcca gtgctcatcg gaacgcgatc
     3481 cgtcgaaacg agcgagcttc tcagcaggat gttgaagcgc aagggcatcg cccactccgt
     3541 tttgaacgcc aaataccacc agatagaagc ggaaatcatt gcgaaggccg ggcagcccga
     3601 cgcggtgaca atcgcgacga acatggcggg ccgaggcacc gacatcaaga ttccgagaag
     3661 cctcatcaaa tgcgacgagt gcaagcttcg cagcggcctt cctgaaagca caatcactgc
     3721 tgacggcatg acgatcaaag actgtctgga aaaacaaccc tgtggcttgc acatcatcgg
     3781 aacggaacgg cacgacgcgc gccgtatcga ccgccagctc agaggcagat ccgggcgaca
     3841 aggtgatccg ggtgcttcgc agttcttcct ttcgctggaa gatgacctca tgcgcctttt
     3901 tggctcggag cgcatcgcga atatcatgac gcgactcggt gctcaggaag gcgaggtaat
     3961 ccaatccccg ctggtgacga aagccatcga tcgaaaccag cgtacgatcg aggcaaacaa
     4021 tttcgagata agaaagcggc tgctggatta cgatgatgtg atgaaccagc agcgcgaagt
     4081 catttatgac cgccgccggt ttgctctgga gaatgacgat ttgaccggcg agatccggtc
     4141 gctgaccatg aacgcgattg atgacatcat cacccgattt gccgaaaacg agaacgatgg
     4201 agagggctgg gatttcggtg cgctgaatgc ggaactgagt aagagattcg gcgtggaggc
     4261 aaacctccgt ttcggaccag aggaaacagt ctctgatgag gtgatccggc agagggtgac
     4321 aaacgcggtt ctctcaaagt acgaagctcg ttgcaacgag cttcggcgcg aggcccgtag
     4381 acaaggtgcg gaggacgaag aggtttttgt gcgccgactc cacaaaatcg cggtcttgtc
     4441 ggctgttgac gaccgctggc gcgaacacct caccgacatc gaggacatga aaggcggaat
     4501 tcaccttcgc cagtatggtc agaaagaccc cgtggtcgaa ttcaaacgtg aggcgtacga
     4561 catattcgaa aacctcatcc gcgaaatcga ccggcagacg ctggaaatag tgttcaagtc
     4621 acaacttccc tcagaacgcg aagtggaagc gcgcagacgc gccgccgcgg agttgatggc
     4681 cacccacgct gagatggaag ggtttgatgc gggagagggc gcgccagtgg ccgtcgcgga
     4741 acccgaaatc cgcacagtgg gcgggcgtgg cgcacggaag ccggtaaccg cggcccgtgc
     4801 ttctgcgccc aaatccgcgc cgcagcagac gaaggccgta cctcgcgtga gccgcaattc
     4861 gccgtgcccg tgcgggagtg gcaagaagta caagtattgc catggaaagg acgagacttg
     4921 accacaaaga ccgggttgtc gagaccccaa acggccttga cgatccttgc tgcagtcggc
     4981 tttccgattt ccctttatct gatcttcgtg gtgaccccgc tcgatgcaga actgggattc
     5041 acgcagaaga tcttctattt ccatgtgccg tgcgcatgga tcacatacct cgcctttttc
//
LOCUS       DAOTNG010000230         5088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13620_length_5088_cov_4.633221, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000230 DAOTNG010000000
VERSION     DAOTNG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13620_length_5088_cov_4.633221"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="P5179_10705"
     CDS             <1..728
                     /locus_tag="P5179_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRS95730.1"
                     /translation="QQIVPLGGHVRRALMVEDDREEIIDAVNSAISRRTAFVITTGGL
                     GPTPDDLTSECLADIAGTVPVPHEPTLDEFVRRRNLKGREELTWNLIRMATVPKTATV
                     LPNPIGWAPCVKLQIGNGTGKTTVFAVPGPPNEMQGVFTAHIAPEIAKATGICRLAER
                     VTIDMWESEASGLFQEVMRRFPGSFLKGYIAMRSAPEDRLPVDIIVAHSDPEEAQRRL
                     YDAVTLFGELVTAAGRTMERGKS"
     gene            840..1481
                     /locus_tag="P5179_10710"
     CDS             840..1481
                     /locus_tag="P5179_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95731.1"
                     /translation="MPPESSSRSVHIPVQKRGSTFEFLYGGSLPEIRDGTKGYLIVPL
                     DSLSPEDRAAFEKLHTEPLLPRGTRLLVLVQATVFADELLIPSSEAFAIPVDGAFVEV
                     ELQEDLTILLRGTKPPILSSCPCYIPRLDIQARSLNHAYSLISERYEPHRASHTGNVF
                     QKVFHRSADGTWEPLEALRKAAQKSEEERLGEHRRKVAGTTSWFARVASRLKT"
     gene            complement(1478..2092)
                     /locus_tag="P5179_10715"
     CDS             complement(1478..2092)
                     /locus_tag="P5179_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRS95732.1"
                     /translation="MVRRFIPRDSYARKASEQGYLARSAFKLERINEKFHVLRPAFRV
                     LDLGAAPGSWLQVASREVGPRGLVVGVDLTPVSFSAPNVVPLTEDILSPDFPDVILPY
                     APFDTVLSDAAPRTTGIRDRDQAGSLALVEAAAGTARSVLKRGGALVAKAFESEDIHR
                     LVRALGNDYASVRTFKPPASRDRSYETYIIAQGKKEQAALEKSG"
     gene            complement(2097..3692)
                     /locus_tag="P5179_10720"
     CDS             complement(2097..3692)
                     /locus_tag="P5179_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS95733.1"
                     /translation="MAKVLVIDDSEPLLDYYGKILKDIGCEVLTTSLGEEGIRLAREH
                     APDCIMLDIMMSDMDGLDVLQELADFDSTIPVIVVTGMPSAESAIEALKRGAFDFLTK
                     GCTVEEIIATARRALDRRRLYLENQELVAKLREANSRLEQQISEATEQVRELNNFNQS
                     LLEGIDAGLLAASESGVILFANAAARRMLSLSPHQVIGSNLRDFGFEVTRAAHTVRRS
                     ETPHIVNGHFEDSLDSVEKKLERVQKRTTYRLPDGSVRVFGYSVSTTRDDDPTGSGRR
                     SIVLFRDMTDMEELRNQMQRLQKLEALNVVVAGVAHEIKNPIAGIKSVASILVETMKE
                     DDPLRDHVLRILEESRRVTRLVEEFFSFSRPSKPKLEAADVSDIIGRVVRLTHDAAKQ
                     RRADIATVVALDIPRVPMDKDQIQQVILNLVLNALEAIGQDGRIEISADVVDYHVLGR
                     RCARVQVRDSGPGFPADVFHRLFDPFFTTKPSGTGLGLHISQNIVLEHGGRIEAANRP
                     DGGAVLSVYLPLEGPSPAAVAAA"
     gene            3873..3946
                     /locus_tag="P5179_10725"
     tRNA            3873..3946
                     /locus_tag="P5179_10725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3905..3907,aa:Gln,seq:ctg)
     gene            4125..>5088
                     /locus_tag="P5179_10730"
     CDS             4125..>5088
                     /locus_tag="P5179_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95734.1"
                     /translation="MNAVRVPQSVVGIIGRGATVTLRSPDTIRVEPAAVPDGLRPREA
                     NTRGTGEVPVHFAASRRIEKGMLRLRVTNETTGDTGEISLPVRVWGPRMSFICLVQSS
                     NTHVGWDPRPMAQYYRRLDEDLVLYDDDKSGNHAWVHARKLERIFHKHGTPVTWLIDD
                     TVAAEQAPQIAEWHGKYGDGVSFLPRSYFYYNKRNYNLTHTPEDTREILTPQYAALLD
                     AFERAGWPVYCRTMGADQWVGSPGGVFVEAAKQMGFEGIWGIGYDHQTCDTSMYHMGA
                     PWDVFKPKAGNFRIPGAGSAFWCFQWTTGYREYLLLPPHGRLHHL"
BASE COUNT          975 a         1608 c         1508 g          997 t
ORIGIN      
        1 aacagcagat cgttcctctc gggggacacg tccgccgtgc cctgatggtg gaggacgacc
       61 gggaagagat cattgatgcg gtgaacagcg caatctcccg gcgcacggcc ttcgtcatca
      121 ccacgggagg gctggggccc acccccgacg acctcacctc cgaatgcctt gccgacattg
      181 cgggaaccgt acctgttccc cacgagccaa cgctggacga attcgtgcgc cgaaggaacc
      241 tgaaaggccg cgaggaactc acctggaacc tgatacgcat ggcgacggtt cccaaaaccg
      301 ccaccgttct gccaaatccg atcggctggg caccgtgcgt aaagctgcaa atcggcaacg
      361 gcaccggcaa aacaacggtg ttcgcggttc ccggtccccc caacgaaatg cagggcgtgt
      421 ttaccgcgca catcgcgccg gagatcgcaa aagcgacggg gatttgccgc cttgccgaac
      481 gagtgacgat tgacatgtgg gaatcggaag cttcggggtt gtttcaggaa gtcatgcggc
      541 gcttccccgg ttcgtttctc aaggggtata tcgccatgag gtccgctcct gaggatcgcc
      601 tccccgtgga tatcattgtc gctcacagtg atccggagga ggcacagcga aggctttatg
      661 atgcagtcac gcttttcggg gagctggtga cggcggcggg ccggacgatg gaacggggga
      721 aatcgtagtg cccgttcccc ttttcccgcg gtcaggattt gcatcttcgc cgcgtgtgca
      781 tgaacttaaa ggagcggcaa tttcagggtg agcttcgggt gcgcgaaagg gtggtgcgta
      841 tgcctccaga gtcgtcatcc agaagtgtcc acatccccgt gcagaaaagg ggttctacct
      901 tcgagtttct gtacggcggc agcctgccgg aaattcgcga cgggacgaag gggtacctca
      961 ttgtgccgct ggattcactc agccccgagg atcgcgccgc gttcgagaag cttcacaccg
     1021 agcctctgct ccccaggggc acccgtcttc tggttctggt gcaggcgacg gtgtttgccg
     1081 acgagttgtt gataccctcc tccgaggcat ttgccatccc tgtggacggg gcgtttgtgg
     1141 aggttgagct gcaggaggac ctcacaatcc tcctccgcgg aaccaaaccc ccgattctct
     1201 cttcctgtcc gtgctacatc ccccgtctgg atatccaggc tcgcagcctc aaccacgcct
     1261 attcgctgat ttccgagcgc tacgagccgc accgcgcgtc gcacaccggc aacgtgtttc
     1321 agaaagtgtt tcaccgttct gcggacggaa cctgggagcc gctagaagcg ctgcggaagg
     1381 ctgcccagaa aagcgaagag gaaagactcg gggaacaccg caggaaagtt gccgggacca
     1441 caagctggtt cgcgcgcgtt gccagtcggc tcaaaaccta accgcttttt tccagagccg
     1501 cctgttcctt tttcccctgc gcgatgatgt aggtttcgta gctccggtct cgcgaggcgg
     1561 gtggcttgaa cgtacgaacg ctcgcatagt catttccaag cgcgcgcacc agccggtgaa
     1621 tgtcctcgct ctcgaatgcc tttgccacga gcgcgccccc gcgcttcaga acggagcgcg
     1681 cagtgcccgc cgccgcctcc acaagcgcca gcgagccggc ctggtcccga tcacgaatcc
     1741 ccgtcgtgcg gggggctgca tcgctcagaa ccgtgtcgaa cggcgcatac gggagaatga
     1801 cgtcagggaa atccggactc agaatgtctt ccgtcagggg tactacgttc ggtgcggaga
     1861 atgagaccgg ggtcagatca acacccacca ccagcccccg cggtcccact tcgcggctgg
     1921 cgacctgaag ccagcttccc ggcgccgcgc cgagatccag aacgcggaag gcaggacgca
     1981 ggacgtggaa cttttcgttg atgcgctcca gcttgaaggc gctgcgtgca aggtaaccct
     2041 gttctgacgc cttccgggcg tacgagtcgc ggggtatgaa acgtcttacc atgcggtcag
     2101 gcggccgcaa ccgccgccgg ggaggggcct tccagcggaa gatacacgct cagcactgcg
     2161 cccccgtccg ggcgatttgc cgcctcgatg cgcccgccgt gctcaagcac gatgttctgg
     2221 gatatatgga ggcccagccc ggttccggaa ggtttcgttg tgaagaacgg atcgaacagg
     2281 cggtggaaca cgtccgcggg gaaaccggga ccggaatccc gcacctgaac gcgcgcgcat
     2341 cgccggccaa gaacatggta atcgaccaca tcggcggaga tctcgatcct gccatcctgc
     2401 ccgatcgctt caagggcgtt gaggacgaga ttcaggatca cctgctggat ctgatccttg
     2461 tccatcggca cgcgcgggat gtcgagcgcc accacggtgg caatatcggc ccttcgttgc
     2521 ttggcggcgt catgggtgag ccgcaccacg cggccaatga tgtcagaaac gtcagcggct
     2581 tcgagtttcg gtttcgatgg gcgcgaaaac gagaaaaact cttccacaag acgtgttacg
     2641 cggcgtgatt cttcgagaat acggaggacg tgatctcgga gcgggtcgtc ttccttcatg
     2701 gtttccacca gaatcgacgc cacgcttttg atcccggcga tcgggttttt gatctcgtgc
     2761 gccacaccgg ctaccaccac gttcagggct tccagcttct gcaaccgttg catctggttg
     2821 cggagctctt ccatgtcggt catgtcgcgg aacaggacga tcgaacgccg tcccgaaccg
     2881 gtcgggtcgt cgtcccgcgt agtggaaacg gaatacccga acacgcggac ggatccgtcg
     2941 ggcaggcggt acgtcgtacg cttctgcaca cgttcgagct tcttctccac ggagtcgagg
     3001 ctgtcttcaa agtgcccgtt cacgatatgg ggtgtctcgc tccgccgcac tgtgtgagcg
     3061 gcccgggtaa cctcaaaccc gaaatcccgg agattggatc cgataacctg gtggggactc
     3121 agcgacaaca tcctgcgcgc agcggcgttc gcgaaaagaa tcactccgga ttcgctcgcc
     3181 gccagcagcc ctgcgtcaat gccttccaga aggctctggt tgaagttgtt cagttcccgc
     3241 acctgctcgg tcgcttcact tatctgctgt tccaggcggg agttggcctc cctcagtttg
     3301 gcaaccagtt cctggttctc caggtaaagc cttcgccggt ccagcgcgcg ccgtgcggtg
     3361 gcgatgattt cttctaccgt gcatcccttc gtaaggaagt cgaacgcacc ccgtttgagg
     3421 gcttcaatgg cgctctccgc gctcggcata cccgtcacca caataaccgg gatggtggaa
     3481 tcaaaatccg ccagttcctg cagcacatcg agtccgtcca tatccgacat catgatgtcc
     3541 agcatgatgc aatccggcgc atgttcacgc gccagccgga ttccctcctc acccagcgat
     3601 gtggtgagca cctcgcagcc gatgtccttc aggattttgc cgtaataatc cagcaacggc
     3661 tcactgtcgt cgatgacaag gactttcgcc atgctgcact ccgtcaactg gttccccgca
     3721 acgaggttcg cgcgtgtgcc ttgcgcgctt agctattaaa ttactacaac tttgcggaca
     3781 agtgttacgc acccggcgaa attgcgaaag gccgcggtcc ggagcagcgt tcagttgacc
     3841 gaacccggcg gcggcggtat attttgtatc gctgccccct cgtccaacgg caagacacat
     3901 gactctggat catggtatcg gggttcgaat ccctgggggg caaccagttt tgagggctcc
     3961 cggtcgctga tcgggagccc tttttcgtgc gggtgcctcc ctctgcgccg ttcccttgcc
     4021 tccctcccgt tgacgcacac taccgcgtgc ccgattctta atcctcaacg caccccctga
     4081 attcccgtgc atcccttgcc agaacgcaca aagggtttcg ccccatgaat gctgttcgcg
     4141 ttccccagtc tgttgtcggc atcatcggcc gtggtgccac ggtaaccctc cgctcccccg
     4201 ataccatccg cgtcgaaccg gcggcggtgc cggatggtct gcgtccgcgc gaggcaaaca
     4261 ccagaggcac cggggaggtg cccgttcact tcgcggcaag caggcggatc gaaaagggga
     4321 tgctgcgact ccgcgttacg aacgaaacta ccggtgacac cggcgagatc tcacttcccg
     4381 tgcgggtctg ggggccgcgc atgagcttca tctgcctcgt gcaatcgagc aacacgcacg
     4441 tgggatggga tccccgcccc atggcgcagt attaccgccg cctcgatgaa gaccttgtcc
     4501 tctatgacga cgacaagagc ggcaaccatg cctgggtgca cgcccgcaag ctcgagcgca
     4561 ttttccacaa acacggcacc ccggtcacct ggctgataga cgacaccgtg gcggcagagc
     4621 aagcgccgca gatcgccgaa tggcacggga agtacgggga cggcgtctca tttctgccgc
     4681 gctcgtattt ctactacaac aagcgcaatt acaacctcac tcacacgccg gaggacacgc
     4741 gggagatcct cactccgcaa tatgctgcgc tgcttgatgc gttcgaacgc gcgggatggc
     4801 cggtgtactg ccggaccatg ggggcagacc agtgggtggg gtctcccggc ggggtgttcg
     4861 tcgaagcggc taaacagatg ggattcgagg gaatctgggg catcggatac gaccaccaga
     4921 cctgcgacac cagcatgtac cacatgggcg cgccgtggga cgtattcaag ccgaaggcgg
     4981 gtaacttccg catccccggc gccggcagcg cgttctggtg cttccagtgg accacgggat
     5041 atcgtgaata cctcctactt ccgccccacg gtcggctcca ccaccttc
//
LOCUS       DAOTNG010000231         5087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13625_length_5087_cov_4.947933, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000231 DAOTNG010000000
VERSION     DAOTNG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13625_length_5087_cov_4.947933"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="P5179_10735"
     CDS             complement(<1..229)
                     /locus_tag="P5179_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS95735.1"
                     /translation="MRVAFSLILLIWAGIHFYVGIRMIPPARLSKRASITAWVALALV
                     ASLVPLSFTVGRSPSAPEWFHLVQTAGFVAMG"
     gene            423..815
                     /locus_tag="P5179_10740"
     CDS             423..815
                     /locus_tag="P5179_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95736.1"
                     /translation="MPDPFPFSLRPAPKRGLVLTFILLAMMIVYGMTATGVLAYYLAP
                     ERAPQLPAALDPRLFPFVGLLALLKTAACIAVWRWQRWGVYVLLITAGAMVVADLSLP
                     QPVLGSFVDALGALLVLVLCRRTWEDFA"
     gene            812..1723
                     /locus_tag="P5179_10745"
     CDS             812..1723
                     /locus_tag="P5179_10745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS95737.1"
                     /translation="MTESPPLVSVVMPTHNSVSHGFLRPAIESVLGQTWRNLELIIVD
                     DGSVDGTPDFVRQEYASDARIKLFELEHVNQANARNYGVAHSSGRYIAFLDSDDTWKP
                     EKLERCLLKFEEGPVPFSGLLFHPMDTIDERGTIRKTRAAQTRPLAYTELLEHNPIAC
                     SSVVVPKQVLENAGPMRLDPPCSEDYDLWLRVAVEYPVLWLGEVLGASRVHQGNVSRK
                     TDLMEQAELTVIGERLRLLTNREADRIRARHLSSFARMRFGNNDFAGFQKHYATLRSL
                     GHVDWPLRIRYALSLMPCILRMIRRAP"
     gene            1977..2501
                     /locus_tag="P5179_10750"
     CDS             1977..2501
                     /locus_tag="P5179_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HRS95738.1"
                     /translation="MDVGSRIDGFIAHLAAFREVEVFDIRPLAVPIPGVTFRQVDLMA
                     PGADFSGLEGYCDSLSCLHALEHFGLGRYGDEIDPDGFEHGFANMARMLSDGGTFYLS
                     VPIGINRIEFNAHRVSDPRMIVDMAERNGLLLSSLTTVKDGREVKRATREDLADLARA
                     RYVLGVFVLVKKQS"
     gene            2509..3390
                     /locus_tag="P5179_10755"
     CDS             2509..3390
                     /locus_tag="P5179_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HRS95739.1"
                     /translation="MVIVQLTGGLGTQLFQYAAARAIAHRNGVPLKLDTSWFKRYPQR
                     AFALGGFMIEASVATTEEIEELCPPRWSLRWAGGQARDRTKPYSRRRVVRERWKRFDP
                     ALLRVGGDAYLIGCWQSERYFTDIAELIRRELVFKTPPDERNAELLSRIDAIDSVSLH
                     IRRGDYVTNPVLADIYGTPGLQYYARALEVVAGHTDAPHVFVFSDDIPWARENLVTNL
                     PTDFVCHNGPGKEIEDLRLMSRCRHHIIANSSFSWWGAWLAEHPGQLVIAPDPWFRDR
                     KWAPADIVPERWTTLPA"
     gene            3632..4927
                     /locus_tag="P5179_10760"
     CDS             3632..4927
                     /locus_tag="P5179_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS95740.1"
                     /translation="MNGTYETLRADIASIPVIDCHEHTNGPASGPTYSDPLAALVVGY
                     VNSDLQSAGTEPVLAASMDTSKPLEERWKLFEDIWKRTQHTSYARVTKWVLREMYGEK
                     ELSLDALKRMAGNLLNLKDEKTYWGILDKAGIKCRIVNIWVGDRKIIEREHVLPDRDR
                     YMIGLPGYHSIHSWNEVYSHTQQIGRTAASLDEYIDICREIFTRLKGRGAIGMKDQSA
                     YNRTLQFDNATREAAEKVFNFFMEDPRRSVGYPESKPLDDYLFHAFMRIARDLDMPVQ
                     IHTGHMAGVRNDIAKTNAVHFTSVLELHREVRFDLFHGNWPYMGEWLYLGKNFPNVAL
                     DCCWLHIIDSIYAQNVLAESVAAVPHGKIHGFGGDYGDSVEFAAGHLSIARDNIAAAL
                     ARLVDSGWIGMDDARQIAADWLFNNPNRFFRLGFEPVTV"
BASE COUNT         1070 a         1409 c         1544 g         1064 t
ORIGIN      
        1 atcccatagc cacgaatccg gccgtctgaa cgagatggaa ccattcgggg gctgagggtg
       61 acctcccaac ggtgaaagac aacggcacga gcgaagcaac gagggcgagc gcgacccacg
      121 cagtaatgga ggctctcttt gagagcctgg cgggcggaat catcctgatg ccgacgtaaa
      181 agtggatacc ggcccagatg agaagaatga gtgagaaagc aacccgcata gtccggagaa
      241 tctcgcggtg aggtcacgtg aaacatccgc tcgtacgatc aactagaacg ttattcctcg
      301 cagcgcagtt cccccagttg ctccgcatcc gactcgccat gtgctatctt ttgtgcaacg
      361 gtggcgtcgc aaagggtgtt ttgaagaggg ccacacaact cggcataaca agagccagac
      421 ccatgccgga cccgtttcct ttctcgttgc gtcccgcgcc gaaacgcggg cttgtgctca
      481 ccttcattct tctggcgatg atgatcgtgt acggaatgac cgcgaccggc gtactggcgt
      541 actatctggc tccggaaagg gcgccgcaac tgcctgctgc gctggatccg cgtctttttc
      601 cttttgtggg tctgcttgcg cttctcaaaa cggcagcctg cattgccgtg tggcggtggc
      661 aacgctgggg tgtgtatgtg ctcctgataa ccgccggagc gatggtggtg gctgacctgt
      721 ctcttccgca gccggtgctc gggtcgtttg tggatgccct cggcgccttg ctggtgcttg
      781 tcctgtgtcg gcgaacctgg gaggatttcg cgtgacggaa tcaccccccc ttgtctctgt
      841 tgtgatgccg acgcacaatt cggtctctca cgggtttctg cggccggcga tcgaaagcgt
      901 tctcgggcag acgtggagaa acctggaact gatcatcgtc gacgacgggt ccgttgacgg
      961 cacgcccgat ttcgtcaggc aggaatacgc gtctgacgca agaatcaagc tgttcgagct
     1021 ggaacacgtg aaccaggcga atgcacgcaa ctacggggtc gcgcactcct cggggcggta
     1081 catcgcgttc ctggattccg acgacacgtg gaaaccggaa aaactcgaac ggtgcctgct
     1141 caagttcgaa gaaggtcccg tccctttttc cgggttgctt ttccatccca tggataccat
     1201 tgatgaaagg ggaaccattc ggaaaacgcg ggccgctcag acgaggccgc ttgcatacac
     1261 ggaattgctg gaacacaatc cgatagcctg ttcgtctgtg gtcgtcccga agcaagtctt
     1321 ggagaacgca ggacccatgc ggctggatcc tccttgttcg gaggattacg atttgtggct
     1381 ccgagtcgcc gtggagtatc cggtcctgtg gctgggcgag gtacttggag cctcacgggt
     1441 acatcaggga aatgtctcgc gtaagacaga tctgatggag caggcggagt tgacggtaat
     1501 tggtgagcga ctgcgccttt tgacgaatcg cgaagcggat cggattcgcg caaggcattt
     1561 atcttctttt gcgcggatgc ggttcggaaa caatgacttt gccggttttc agaagcatta
     1621 cgcaacactg aggtcgctcg gtcacgtgga ctggccgctc aggatacggt acgcgttgtc
     1681 attgatgcct tgtattctga ggatgatacg gagggcaccg tgatcaccgt ccggcgcctg
     1741 cactggcttt cgattacgca actggggttt gaccccaaag tcactttccg gtccctgaaa
     1801 gccttgcctc ggtatgtgaa aggatattgg acgctgaggg gcaagtacga ggggccggtg
     1861 gagttcaccc cgtgcctgca ggactggttc aaacaggcgg gcgagacgga aagcgactat
     1921 ttctggcaag accttattgt tgcgcgaatg attttcgaga aaaaccaagg aagcatgtgg
     1981 acgtcgggtc ccgtattgac ggattcatcg cgcatttggc ggccttccgc gaggtcgagg
     2041 tgttcgacat acgtccgctt gcggtgccaa ttccgggggt aaccttccgg caagttgacc
     2101 tcatggcgcc cggtgcggac ttcagcggac ttgaggggta ctgcgattcg ctctcctgcc
     2161 tccatgcgct ggaacatttc ggcctcggcc gctatggcga cgaaatcgat ccggacggct
     2221 tcgaacacgg ctttgcaaac atggcgcgca tgttatccga cggaggcact ttttaccttt
     2281 cagttcccat cggaatcaat cggatcgaat tcaacgcgca tcgcgtgtcg gacccgcgaa
     2341 tgatcgtcga tatggccgaa cggaacggac ttctgctttc gtcgctcaca accgtgaaag
     2401 atggcagaga agtgaaaaga gccacgcgtg aggacctagc tgacctggca cgcgcgcggt
     2461 acgtgctcgg agtgttcgtg ctggtcaaaa aacaatcctg aggccgcgat ggttattgtt
     2521 cagctcacgg gagggctggg aactcagctt tttcagtacg ccgccgcccg ggcaatagca
     2581 cacagaaacg gcgttccact gaaactggac acctcctggt tcaaacgtta cccgcaacgc
     2641 gcattcgcgc ttggcggctt catgattgaa gccagtgtag ccacgacgga ggaaatcgag
     2701 gagctatgtc cgccgcgctg gagcctccgc tgggcgggtg gacaggcgcg ggaccgaaca
     2761 aagccctact cgcgcaggcg tgtggtcagg gaacggtgga aacggtttga tccagcactg
     2821 ctcagagtgg gaggggatgc gtatctgatc ggatgctggc agtccgagcg gtatttcacg
     2881 gatattgctg aactgatccg ccgggagttg gtcttcaaaa ccccgccaga cgagagaaac
     2941 gcagaactgc tctcccgaat agacgcaatc gactccgtga gcctgcacat acggcgtggc
     3001 gattacgtga caaatcccgt tcttgcagat atctacggca cacccggtct ccagtattac
     3061 gcacgagctc tggaggtagt tgccgggcac acggatgcgc cccacgtctt tgtgttctct
     3121 gatgatattc cgtgggcccg ggagaacctt gttacaaatc tccccacgga tttcgtctgt
     3181 cataacggtc ccgggaagga aatcgaggac ttgcgcctca tgagcaggtg ccgtcaccac
     3241 attattgcga acagctcgtt cagttggtgg ggcgcgtggt tggcggagca ccccgggcag
     3301 ttggttattg cgccagaccc gtggtttcgc gaccgcaaat gggcgcccgc ggacattgtg
     3361 ccggaaagat ggacgacctt gccggcgtga aggcgagggt cgggtatcca cgcaaggtac
     3421 gaaccattga tatggcggcc agcctgcgtg tctgttggtg cgcttcctcc cctgctttat
     3481 tatccactgg aacaaactgg tgcgccgtgg ctcacgacgc acaacgcaga atcaatgttg
     3541 cggcgttgaa cacattgaca taagcggagt tatcgtttct ttcagtcggt gaactgataa
     3601 ccagcctcat ccatccggaa ggagaaccca tatgaacggc acatacgaaa cactccgcgc
     3661 ggacatcgcc tccattcctg tgattgactg ccacgagcac acaaatggac cggcgagcgg
     3721 gccgacgtac agcgatccgc tggccgcgct ggtcgtcggg tacgtgaact ccgaccttca
     3781 atctgcggga acggaacctg ttctggcagc ctccatggac acttcgaagc cgctggaaga
     3841 gcggtggaag ctgtttgaag atatctggaa acgtacacag cacacgtcgt acgcccgcgt
     3901 tacgaagtgg gtgctccgcg aaatgtacgg tgaaaaggag ctttcccttg atgcgctcaa
     3961 gcgcatggcg ggaaacctgc tcaacctgaa ggacgaaaaa acctactggg gcattctcga
     4021 caaagccgga atcaaatgtc gcatcgtcaa tatctgggtc ggagacagga agatcatcga
     4081 acgggagcac gtcctgccgg accgcgaccg gtacatgata gggctgcccg gatatcacag
     4141 catccactcg tggaacgagg tgtacagcca cacccagcag atcggccgga cggctgcctc
     4201 gctcgacgag tatatcgaca tctgccgcga gatattcact cgcctcaaag gtcgcggcgc
     4261 catcgggatg aaggatcaat cggcgtacaa caggacgctg caattcgaca acgccacccg
     4321 ggaggcggcg gaaaaggttt tcaacttctt tatggaagac ccacgccgtt ctgtcgggta
     4381 ccccgaaagc aaaccgctcg acgattacct tttccacgcg ttcatgcgta tcgcgcgtga
     4441 tctggatatg ccggttcaga tccacaccgg acacatggca ggcgttcgca acgacatagc
     4501 caaaacgaac gcggtgcatt tcaccagtgt gctggaactg caccgcgagg tccgctttga
     4561 tctgttccac ggcaactggc cgtacatggg cgagtggctc tacctgggca agaatttccc
     4621 gaacgtggcg ctggattgct gctggctcca tatcattgac tcgatctatg cacagaacgt
     4681 gcttgccgag tcggtggcgg cagtgccgca cggcaagatt catggattcg ggggcgatta
     4741 cggggattcc gtggagtttg cggcggggca tctttcgatc gcccgggaca acattgccgc
     4801 agccctggct cgactggtcg attccggctg gatcggtatg gacgatgccc gacagattgc
     4861 cgccgactgg ttgttcaata acccgaatcg tttcttccgc ctgggttttg agccggtgac
     4921 agtgtgaggc acgccgcgcg cgtaagacca ggctgacgtg gccagtgccc gggggtgccc
     4981 gttgtgcgcc gccgtacgcg agaaagaagc cggacgcgat gagacaaacc tttccaacgc
     5041 cgggggaaag gcgcttcacc cggggggtgg tgctggcgct ttccgac
//
LOCUS       DAOTNG010000232         5087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13626_length_5087_cov_4.645469, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000232 DAOTNG010000000
VERSION     DAOTNG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_5087_cov_4.645469"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1648)
                     /locus_tag="P5179_10765"
     CDS             complement(296..1648)
                     /locus_tag="P5179_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS95741.1"
                     /translation="MRKVIVAWCVGVVFFDAVAGAPAVGMFRSLGASPLWIGLLAAIP
                     PIATLAQPVASYIAARTGSRKRLFLRSAYPGRVIWLPIVLCGYFLPRGDLSIAFLFVL
                     VFFSKLSDSISGPAWMAWISDLVPENERGRFWGRRQMWASLCSVTGSLCLGYYLGTQP
                     PYPKFIVFFGIVAFLGWLDVFVHRAVPGVRLDVPSKRPDFRKLVTVPLRERSYRSLVL
                     FYGLFSFSVAVGGALFPLLLIEEFGFSYFKISFFFGIQAGFMIASSHLWGRFMDNVRE
                     GPRVVFLMCSVIIALLAPPWTMIPVGQNLLAGLNFALGGIGWGGYQIAFTGLVIAMSP
                     RESRAMYFALAAVAAGIGNAVGAVCAGLIAEQLAGFSFFWGPFKLTQYRTLYLISGIS
                     RMSCLLLLPLIRQPESAPVGTYFRRAMSLNPMEPGTYAYLWKKFGKPNGNEQRGETEN
                     "
     gene            complement(1799..3004)
                     /locus_tag="P5179_10770"
     CDS             complement(1799..3004)
                     /locus_tag="P5179_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95742.1"
                     /translation="MPSLRIRGLVRAVRYVETRMRGRLTLKDADRLAHYVRSVVAEVR
                     AICQNYGTSPEALPRPSREAFAFLNGLDAAHLDRAKPCRVAISTPVRISSVVRLTGAV
                     LTDLGSGTEDESSECFVERCHARVLAAAGEIEALCRARGVDPREMAVRSRRAYGLLRY
                     LSNVQTTRAYVRTAANLKRICGELRRREGADSSAPVVTLDDHSHIYRCGRGKDAPLVL
                     HLSPAFIGAPEEVLESVARLAFGIGAASDKKAVHTFIHGPGALAIIQEIAELTSGPSE
                     TRGAVYDLREVCERVRNRYFDDPPDPPSSLAWTDSLTYRMFGLYSSVRDRITISRSLD
                     SPKVPGYVIDYVMFHELLHKKHGIGWASKQRTMHTSRFRREEHAFPRFKAAQEFLNRL
                     SSQMRRRLT"
     gene            complement(3065..4213)
                     /locus_tag="P5179_10775"
     CDS             complement(3065..4213)
                     /locus_tag="P5179_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS95743.1"
                     /translation="MALVDLRGQKKRRTPEEMIAHLNSFSLDLAFSAGVWFFAPGGGR
                     FHDRYTPNLTIKERLEVAAELSRYGLKAVEAHYPNEVNEDNVHLYKDLEKNAGVRLAT
                     VIPGLFFDSEFEWGSLSSPIPSVRKKAIDRTIAALKLNKELNTDFAIVWPGIDGYENP
                     FGMDMVAARDRFAEGLAEAMDAVPGIRVAEEPKPYEPRGHILYGTTAEGLLLCAKVES
                     LLKAPENVKLLAEGHAMMCMNPEVGHMLMGFEDAPYSFSLIMEYGRLAHTHWNSQPLG
                     NYDQDLNVGVLSPESTEASLYVLKMYGYKGYFGPDINPERMPVQQALKNCFDALRAAN
                     ERINNLDHEKIIMATSHPEKNRGWLEAYLIRERAPKSISLPPLPPFEK"
     gene            complement(4390..>5087)
                     /locus_tag="P5179_10780"
     CDS             complement(4390..>5087)
                     /locus_tag="P5179_10780"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRS95744.1"
                     /translation="KKPWWSGTKSDMHVINDWWNGYAMEVDKAAHAFAGQSMARLAAE
                     SYEWTGMTRTQALFWGGVTSLATLSQVEVLDGYTAKFGFSTADYAANIAGAFFPLAQE
                     MWHPLKTVTFKISYHTRRFEEHGNNGNILEDYDRQTYWLVLNLKETLPRTVSSVVPAW
                     LGIAAGYGVENAFASTGRMREYYLALDIDPTRANVGPGWLSRALAPLRYVHLPSPAVR
                     FREDGTRFFALYF"
BASE COUNT         1110 a         1537 c         1449 g          991 t
ORIGIN      
        1 tccgttcgca gagggtgcgc agttccgggt agaaccgcaa cccttccaga ggcaacatgg
       61 cggcctttgt agcgccgagg cgacgggctt cggcgacaag gtcaagatgc ttcagttcca
      121 tcagaatcac gtccgcgcga tgcaggtctg gtgcgaatct cgttcagcaa taacagtgta
      181 ccgcaaccaa tttgttccag agaacggcgg tccggagcag ggcaggcaaa accccggacg
      241 gcagcgcctg ccgaacgcga aaatcaaaag agttccgttc cgggcatgcg gcacgtcagt
      301 tttcggtctc tccacgctgc tcgttgccgt tcggttttcc gaatttcttc cagagatagg
      361 cgtacgttcc cggttccatt ggattgagac tcattgcccg tcggaagtat gtcccgacag
      421 gcgccgactc cggttggcgg atgagaggca gcaggagcag gcaggacatg cgcgagatac
      481 cggaaatcag atacaaggtt cgatactgag tcagtttgaa ggggccccag aaaaaggaga
      541 accctgcgag ttgttcggcg atcaaccccg cgcatacggc gcccacagcg ttgccaatgc
      601 cggcggcgac ggccgcaagc gcgaagtaca ttgcccgcga ctcgcgggga gacatcgcaa
      661 ttacaagccc ggtgaacgcg atctggtacc ctccccagcc gatcccgcca agggcgaagt
      721 tgagtcccgc gagaaggttc tgaccgaccg ggatcatcgt ccacggcggc gccagcagag
      781 cgatgattac cgaacacatc agaaagacca cgcgaggccc ttcccgcacg ttgtccatga
      841 agcggcccca gagatgggaa ctggcgatca tgaatccggc ctgaatcccg aaaaagaagg
      901 aaatcttgaa atacgagaac ccgaactcct cgatcaaaag aagggggaac agcgcaccgc
      961 cgacggctac cgaaaaggag aagaggccgt agaaaagtac cagcgagcgg tacgagcgct
     1021 ccctcagcgg taccgtcacc agtttacgga aatcaggccg tttcgaaggc acatccagtc
     1081 gcacgcctgg caccgcgcga tggacaaaca cgtccaacca gcccagaaac gcgacaatgc
     1141 cgaaaaacac aatgaatttc gggtaaggag gctgcgttcc gaggtaatat ccgaggcata
     1201 gcgaaccggt tacgctgcag aggcttgccc acatctggcg cctgccccag aagcgtcccc
     1261 gttcattctc cggcaccaga tcggagatcc atgccatcca tgccggcccg gatattgagt
     1321 cgctcaactt gctgaaaaac acaagaacga aaagaaacgc gatcgagaga tcccctcggg
     1381 gcaggaaata cccgcacagg acgatgggca gccagatgac ccgtccggga tacgcacttc
     1441 gcaggaacag ccgtttccgg gaacccgtcc gcgcggcgat gtacgacgcg acaggttgcg
     1501 cgagagtagc gatgggagga atcgccgcca gcaagcctat ccacagcgga gaggctccca
     1561 gagaacggaa cattcccacc gcgggcgcac cggcaacggc atcaaagaac acaaccccga
     1621 cgcaccacgc cacgataacc ttgcgcatgc cgcggtgccg ttcgtcgatt gacagttgct
     1681 cgcccggcag ggtgggtgca tagcgaaacc attcacgcgg cgacgtcaac actgcagtta
     1741 cccagccaga gtgtgatctt gtcgggacaa cggcccggtg aggacggggc aaatcatgtc
     1801 aggtaagccg gcggcgcatc tgagaggaaa gtctgttgag aaactcctgc gcggctttga
     1861 aacggggaaa tgcgtgctcc tcccgtcgga agcgactggt gtgcatcgtg cgttgtttcg
     1921 acgcccagcc gatgccatgc ttcttatgaa gaagctcgtg gaacatcacg taatctatca
     1981 catacccagg caccttcgga ctgtccagag accggctgat tgtaattcta tcgcgaacgg
     2041 agctgtagag cccgaacatt ctgtacgtca gggagtccgt ccacgccagc gaagaaggcg
     2101 gatcaggcgg atcatcgaag tatcggttgc gcacacgttc gcacacttct cgaaggtcgt
     2161 acaccgctcc acgtgtttcg gacggcccgg acgtgagctc ggcaatttcc tgaatgattg
     2221 ccagcgcccc ggggccgtgg atgaatgtat gtaccgcctt cttgtccgaa gcggctccga
     2281 ttccgaacgc caggcgcgcc acagactcca ggacctcctc aggagcgccg atgaacgcgg
     2341 ggctgagatg cagcaccaga ggcgcgtctt tccctcgccc gcaccggtaa atgtggctat
     2401 ggtcatccag ggtcaccacc ggtgcggacg aatcagctcc ttcacgtcgt cttagctcgc
     2461 cgcagatgcg cttcagattc gccgcagtcc tcacgtatgc gcgtgtcgtc tggacgttcg
     2521 acaggtagcg gagaagaccg tacgctcggc gggaccgcac ggccatttca cggggatcaa
     2581 ctcctcgcgc ccggcacaag gcctcgattt cccctgcggc agccagaacg cgggcgtgac
     2641 agcgttccac aaaacactcg ctggattcgt cttctgtgcc ggagcccaga tcggtcagca
     2701 ctgcccctgt caggcgaact actgaggaaa tccggacagg tgtgctgatg gccacgcggc
     2761 agggtttcgc gcggtcgagg tgcgccgcat cgaggccgtt caaaaatgcg aaggcttccc
     2821 ttgaaggccg tggcaacgct tccgggctgg taccgtagtt ctggcatatt gcccgtacct
     2881 ccgcaactac cgaccgaaca tagtgcgcca gcctgtcggc gtctttcaga gtaagcctgc
     2941 cgcgcatacg tgtttcgaca tagcgcacgg ctcggacaag gccacggatt cgcaacgaag
     3001 gcatggatgg gtgccgagag gcaccggccg gggtttcacg ccccgaccgg caccaatcac
     3061 gaggtcattt ttcgaacggt gggagcggcg gcagcgatat gctcttcggc gcgcgctcac
     3121 ggatgagata cgcttcgagc caaccgcggt tcttctcggg gtgtgacgtc gccatgataa
     3181 tcttctcgtg gtccagattg ttgatccgct catttgccgc tctgagcgca tcaaaacagt
     3241 tcttcagcgc ttgctgcacc ggcatgcgtt ccgggttgat atccggcccg aaatacccct
     3301 tgtaaccgta catcttcaga acgtagagcg acgcctctgt cgattccggt gaaagcacgc
     3361 ctacgttcaa atcctggtcg taattgccca gcggctggct gttccagtgc gtatgtgcca
     3421 gtctgccgta ttccatgatc aggctgaagc tgtagggtgc gtcctcaaag cccatcagca
     3481 tgtgaccgac ttcggggttc atgcacatca tcgcgtgccc ctcggccagg agcttcacgt
     3541 tttccggagc cttcagaagc gactcgactt tcgcgcacag aagcagccct tcggcagtcg
     3601 ttccgtaaag aatgtgtcct cgtggttcgt agggtttcgg ttcttcggcc actctgattc
     3661 ccggcactgc gtccatcgcc tcggcaagtc cctcggcaaa ccggtcccgg gcggcaacca
     3721 tatccatgcc aaacggattc tcatatccgt ctattcccgg ccacacgatt gcgaaatccg
     3781 tgttcagttc cttgttcagt ttgagcgctg caatcgtgcg gtcgatagcc tttttccgaa
     3841 cggaggggat tggagaggaa agcgatcccc attcgaattc gctatcgaaa aacaaccccg
     3901 gtattaccgt tgccagacga acgcctgcat tcttctcaag gtctttgtaa agatgcacgt
     3961 tatcttcatt tacttcattc ggatagtgcg cttcgactgc tttcagtccg taacgggaga
     4021 gctccgccgc gacctccagg cgttccttga tcgtcaaatt cggcgtgtag cggtcgtgga
     4081 accgcccacc tcccggcgcg aaaaaccata cgcccgcgga aaacgcaagg tcgagcgaga
     4141 aactgttgag atgagcgatc atctcttccg gggtacgacg tttcttttgt cctcgcaggt
     4201 ctacgagtgc cataggaacc ctccttcttt gcaggatcgg ctctgttcag gtacctacgc
     4261 gtgcctttca gagtcggttg ccgaaatcct tccgtccgtt caacccagca gagtcaaata
     4321 tacatgcaag gcacaaccag acacacggat ccgctggagg gtatgcgccc caggcttggc
     4381 gcacgttcgc tagaagtaga gggcaaaaaa gcgggtgcca tcctccctga atcggacggc
     4441 gggcgagggc aggtgaacgt aacgcaaagg cgccagcgcg cgggaaagcc acccggggcc
     4501 gacgtttgca cgcgtcggat caatatccaa ggcaaggtaa tactcacgca tacgccctgt
     4561 ggaggcgaat gcgttttcaa cgccgtaccc tgccgctatt cccaaccacg caggcacaac
     4621 actgcttacc gtccgtggca gtgtttcctt gaggttgaga accagccagt aggtctggcg
     4681 gtcatagtct tcgaggatat tcccgttgtt tccatgctct tcaaaccgtc gtgtatggta
     4741 gctgattttg aacgttaccg tcttcagagg gtgccacatt tcctgagcga gcgggaaaaa
     4801 cgcgcccgcg atattggccg catagtccgc ggtgctgaaa ccgaacttcg cggtgtaacc
     4861 gtccagaacc tcgacctgac tcaacgttgc aaggctcgtt acgcctcccc agaacagagc
     4921 ctgcgtgcgc gtcattccgg tccactcgta cgattccgcg gccagccgcg ccatggattg
     4981 ccccgcaaag gcgtgcgcag ctttgtccac ctccatcgcg tacccgttcc accagtcatt
     5041 gatgacgtgc atgtcggatt tcgttccgct ccaccagggc tttttca
//
LOCUS       DAOTNG010000233         5066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13694_length_5066_cov_4.040112, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000233 DAOTNG010000000
VERSION     DAOTNG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13694_length_5066_cov_4.040112"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="P5179_10785"
     CDS             <1..1665
                     /locus_tag="P5179_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS95745.1"
                     /translation="WVDRKYDDFMFMHWRIINNGLWSNTGKRTIQQYGGVHDTVRAVM
                     MSLCYQWDDRGRGSNLTNSGGQDNNDGLWHYYGKDYDGARTEDMRLVWVRDGDQDPSK
                     YDAVATGLPSQDDYGDPRPMTGELLSARHMGLMILHWDKAVNDRRDDIAQPMTCGWGE
                     GGGSLDQNWRTARDGHEVKYRQMRYGYQYGDIYYEGDITDTPARGAHPFGGSWIKASN
                     DLPDRETWWPGSIVGVTDEVTQVEQQLGMGPTDIPPNDTLNALYVEAVAGHSLPYAAW
                     VGREWAAGRLTDAQKDAIVDQCQDSLFTVMRRAKAIYESATFPDGHGGTRFASTRDEL
                     HVAYAAAIAAGKLSLSPPAPASFTVVSGKSKVSLYWTLNTTTGSNIAGWRLYKALADF
                     RGDSAWTVVYDGPPNVLGYVDIAVTPGFSYYYYLTTYDANGLESTMHTRTSDAAIPLT
                     GDDNGVLDADRALRFALEQNAPNPFNPTTTIRFSVAASGPTRLDIYSTNGQLIRTLVN
                     GSVAAGVNSVQWDGKDAMGRPVASGTYLYRLTNGTNTSVRRMVLVR"
     assembly_gap    1891..1990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2154..2579
                     /locus_tag="P5179_10790"
     CDS             2154..2579
                     /locus_tag="P5179_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95746.1"
                     /translation="MKQAGIRHVFFALCVGLALPALASAAFAGASADSIAGKVRVVPN
                     PWCASDQAQHYAKRTGNSLFDVKNGYSRVLFVNLPNPCTIRIYTVDGDLVRTIENTAG
                     SSTTWDLITESDQAVTTGIYVWTVDSAVGRDVGKLIIIR"
     gene            2599..3675
                     /locus_tag="P5179_10795"
     CDS             2599..3675
                     /locus_tag="P5179_10795"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS95747.1"
                     /translation="MKSTKYGILVLGAVLAWSLPVKAAGEFDKLGQSGMTFLKIGPVA
                     RAAAMGDAYTGVSTGATGCFYNPAGLGFLTDRVDAIAGQVSWIADIKIASAAAGVPIE
                     LGGNRFAVVGISYVGMDYGTIHGTVINKGSTNGAYSDIGNLEPSEFLLGLTLAKQFTD
                     KFSLGLTGKYVNQGLPWYRFGNATYAVPPPDNLGAVPTVGDSLSEFTARNFVFDAGTL
                     YRTGFGSTVVSMSIRNFSRKTTHVIDAFTTPMTFHIGVAGNAFDPIPALGQMGHKLLI
                     AVDGIHPPDHPEKIALGGEYSFRDMVFVRGGYTFNNDARTWNTGFGVKYGYAGLAGAF
                     DYGYADMGGSLGSVNYFTISFAMQ"
     gene            complement(3741..4937)
                     /locus_tag="P5179_10800"
     CDS             complement(3741..4937)
                     /locus_tag="P5179_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95748.1"
                     /translation="MGISICMVGVGAFAQSFIPLFKAHPLVDAISLCDLDERKLRENS
                     QRYGIPRTYASLDEACDSDVDAVAIITQNWMHAEQAIQALNACKHVYSAVPTGITVDE
                     VVRLVRTVENTGKVYMLGETSYYYPAVIYCREQFRKGAFGRVVYGEGEYYHDWDHGLY
                     DVMKWRGGERWREFAGGPPMHYPTHSTSQIISVTGARMTHVSCCGFVDMHDDGIFDPA
                     VNRWGNPFSNESALFHMSDGSACRINEFRRIGHPGTVRMTLFGTEGSYEENWAGKAWV
                     TKNPSDCIRLDDLLACSGVPALPEKNGMGVVTSQDGTHLHASKVHDVARLPRQFIGLP
                     NGHCGSHQFLVDDFVRACANRTVPPNNVWQAARYALPGIIAHESAMQGGVLLEVPDCG
                     DPPREG"
BASE COUNT          969 a         1437 c         1574 g          986 t
ORIGIN      
        1 tgggttgaca ggaagtacga cgatttcatg ttcatgcact ggcgcatcat caacaacggt
       61 ctgtggagca acacgggcaa gcgcaccatt cagcagtacg gtggcgtgca cgatacggtc
      121 cgcgccgtga tgatgagcct ttgctatcag tgggacgacc gcggccgcgg ctcgaacctg
      181 acgaacagcg gcggtcagga taacaacgac ggtctgtggc actactacgg caaagattac
      241 gacggcgccc ggacggaaga catgcgcctc gtgtgggtgc gtgacggcga tcaggatccg
      301 tccaagtacg atgccgtcgc gacaggtctt ccgagccagg atgactatgg cgacccgcgt
      361 ccaatgacgg gtgagctcct ctcagcccgg cacatgggtc tcatgattct ccactgggat
      421 aaggccgtca acgaccgccg tgacgacatc gcacagccga tgacctgcgg ctggggcgaa
      481 ggcggcggaa gccttgacca gaactggcgc accgcgcgcg atggccatga agtgaagtac
      541 cgccagatgc gctatggcta tcagtacggt gacatctact acgaaggcga catcaccgac
      601 acgccggcgc gtggcgccca tccgttcggt ggttcgtgga tcaaagccag caacgacctg
      661 cccgaccgcg agacatggtg gccgggctcc atcgtcggcg taaccgacga agtgacacag
      721 gttgagcagc agcttggcat gggcccgacc gacattccgc caaacgacac gctgaacgcg
      781 ctgtacgtgg aagcggttgc cgggcacagc ctgccgtatg cggcatgggt tggccgtgag
      841 tgggctgccg gtcggcttac cgacgcgcaa aaagacgcca tcgtggatca gtgccaggac
      901 tcgctgttca ccgtgatgcg ccgggcgaag gcgatctacg aatcggccac cttccccgac
      961 gggcatggcg ggacgcggtt cgcgtccaca cgagacgagt tgcacgttgc atacgcggct
     1021 gccatcgctg cggggaaact ctccctgtcg cctccggcgc ccgcatcctt cacggtcgtg
     1081 tcgggcaaat ccaaggtgag tctgtactgg acgctcaaca ccaccaccgg ttcgaacatc
     1141 gccggctggc ggctgtacaa agcactggcg gacttccggg gcgactcggc gtggacagta
     1201 gtgtacgatg gtcctccgaa tgttctcggc tacgttgaca tagccgtcac gccgggcttc
     1261 tcgtactact attatctcac gacgtacgac gcgaacggtc tcgagtccac gatgcatacg
     1321 cggacctctg atgcggcgat tccgctcacc ggcgatgata acggcgttct ggatgcggat
     1381 cgggcgcttc ggttcgcgct tgagcagaac gcgccgaacc ccttcaaccc caccacgacc
     1441 attcgcttct ccgttgcggc gagtggtccg acccgtcttg acatctacag cacgaacggg
     1501 cagcttatcc gcacgctcgt gaacggttct gtcgcggcgg gtgtgaacag cgttcagtgg
     1561 gatggcaagg atgcgatggg ccgcccggtt gccagcggta cgtatctcta ccgcctgacg
     1621 aacggaacga acaccagcgt ccggcgcatg gtcttggtac gctaggcgaa ctggcgttcg
     1681 cttggcgtac cttggggggg gcgcgatgcg cccccgctgc tgaggcgaac ggcaggtttg
     1741 cgtagaattg agaagcgtaa ggtcatgaga tgagcaaagg gtgcgcgtct ttcggggcgc
     1801 gcaccccgat caccggagtg acggaaccgg gttgagcgaa gggaatacgg cgagcgcacg
     1861 cgtgttcgtt tcgctcaggt cggtttccgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn gcgaagggaa tacggcgagc gcacgcgtgt tcgtttcgct caggtcggtt
     2041 tccgccgcgc ggggaaaggc cgatgccgag gtgggccggg gagatgcaga tttcagggta
     2101 atcgatagaa gcagtatggt attgacgggc aactgcaatc aggaggaacc gcaatgaagc
     2161 aagccgggat caggcatgtg ttcttcgcgc tgtgtgtggg gctggctttg cccgcccttg
     2221 cctcggcggc gttcgcggga gcaagtgcgg acagcattgc gggcaaggtt cgtgtggtgc
     2281 cgaatccctg gtgcgcgtcg gaccaggcgc agcattacgc gaagcgaacc gggaattcgc
     2341 tctttgatgt gaagaacggc tattcccgcg tgctgttcgt gaacctgccg aacccgtgta
     2401 cgatacggat ttacacggta gacggagacc tcgtcagaac gattgaaaac accgcgggct
     2461 ccagcacgac atgggacctg atcaccgaga gcgatcaggc agtgacgacg ggtatctacg
     2521 tctggactgt ggacagcgcg gtcgggcgtg atgtcggcaa actcatcatc atccgctgag
     2581 acgttggagg gatatgccat gaaaagcacg aaatacggaa tactggttct cggtgccgtg
     2641 ctggcgtggt cgctccccgt gaaggcggcg ggtgagttcg acaagctcgg tcaatccggg
     2701 atgacctttc tcaaaattgg cccggtggca cgcgcggcag ccatggggga tgcgtacacc
     2761 ggtgtttcca cgggcgcgac cgggtgcttt tacaacccgg cgggtcttgg gttcctcacc
     2821 gatcgcgtcg acgcaatagc ggggcaggtg agctggattg ccgacatcaa aattgcctct
     2881 gcggcagcag gagttcctat tgaactcggc ggaaaccggt tcgcggtggt aggaatttcc
     2941 tatgttggta tggattacgg cacaattcac ggaacggtga ttaacaaggg ctccaccaac
     3001 ggcgcgtaca gtgacatcgg gaatcttgag ccgtccgagt tcctgcttgg tctgacgctg
     3061 gcgaagcagt tcacggacaa gttttctctg ggcctgaccg ggaagtacgt gaaccagggg
     3121 ttgccgtggt accgcttcgg aaacgccacg tacgcggttc cgccgcctga caatctgggt
     3181 gccgttccta ccgtcggcga ttccctttcc gagtttacgg cacggaactt cgtgttcgat
     3241 gcggggacgt tgtacagaac ggggttcggg agcacggttg tcagcatgag catccggaac
     3301 ttctcccgga aaacgaccca tgtgatcgac gcgttcacca cgccgatgac gttccacatc
     3361 ggggttgcag gaaacgcgtt cgatccgatc cccgcgctgg gtcaaatggg gcacaaactc
     3421 ctgatcgcgg tggacggcat ccatccgccc gaccacccgg agaagattgc gctgggcggg
     3481 gagtattcgt tcagagacat ggtgtttgtt cggggtggat acacgttcaa taacgacgcg
     3541 cgcacgtgga acaccggttt cggcgtgaag tacggatacg ccggtctggc cggtgcattc
     3601 gattacgggt acgcggacat gggaggatct ctcggttccg tgaactactt caccatttcg
     3661 ttcgcgatgc agtaatcgca cctgagcaac gagagaaaaa gccgcatcag agagtctggt
     3721 gcggcttttc gcgttctttg ctatccctcg cgcggggggt ctccacaatc gggcacttcg
     3781 agcaggacgc ctccctgcat ggcggactcg tgcgcgatga ttccggggag ggcgtatcgc
     3841 gcggcctgcc agacgttgtt tggcggtacg gtgcggttgg cgcaggcgcg gacgaagtcg
     3901 tccacgagaa actggtgcga accgcaatgg ccgttcggca acccgatgaa ctgccgcggc
     3961 agccgggcca catcgtgaac ctttgacgcg tgcagatgag tgccgtcctg cgaggtgaca
     4021 acgcccatcc cgtttttctc cggtaatgcg ggcacacccg aacacgccaa aaggtcatcc
     4081 aatcgtatac agtcagacgg gttcttggtt acccacgcct ttcccgccca gttctcctcg
     4141 tagctgccct cggtgccgaa cagtgtcatc cttaccgttc cggggtgtcc gattcgtcgg
     4201 aactcattaa tgcggcatgc gctcccgtcg gacatgtgga acagggcgga ttcgttgctg
     4261 aaggggtttc cccaccggtt gaccgcgggg tcaaatatgc cgtcgtcgtg catgtccacg
     4321 aacccgcagc aggaaacgtg ggtcatacgg gcgccggtaa cggaaatgat ctggctggtg
     4381 gaatgcgtgg ggtaatgcat cggaggtcct ccggcaaact cgcgccagcg ctcgccaccg
     4441 cgccatttca taacgtcgta gagcccgtga tcccagtcgt gatagtattc tccctctccg
     4501 tacactaccc ggccgaatgc tcccttcctg aactgctcgc gacaatagat tactgccggg
     4561 tagtaataac tcgtttcccc gagcatgtag accttgcccg tattttcgac agttcttacc
     4621 agcctgacga cttcgtccac ggttatgccg gtgggcaccg cggaatagac gtgtttgcag
     4681 gcgttcaacg cctgaatagc ctgttcggcg tgcatccagt tctgagtgat gatggcgacg
     4741 gcgtccacgt cgctgtcgca cgcttcatcg agcgaggcat acgtccgggg tattccgtag
     4801 cgttgggagt tttcccggag tttccgctcg tcgagatcgc agagggagat cgcgtccaca
     4861 agcgggtgcg ccttgaaaag cggtatgaag ctctgcgcaa aggcaccgac gccgaccata
     4921 cagatgctga tgcccaagac cgcctcctgt tctcagaccg gggaatcgcc cggtttcccg
     4981 tacgaaaggt cgtaattccc cgtccagctt ccgggatcca cccgctcgac cttcccggtc
     5041 cgcatacttc ggatatttgc gtcaat
//
LOCUS       DAOTNG010000234         5054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13742_length_5054_cov_4.623125, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000234 DAOTNG010000000
VERSION     DAOTNG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13742_length_5054_cov_4.623125"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="P5179_10805"
     CDS             complement(<1..635)
                     /locus_tag="P5179_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRS95749.1"
                     /translation="MRTKSLGFALLFATIALRGAANADDSFRTLTGLESSASSVAITP
                     DGQNVAAGIAGTVKVWRISDGALLSTLGQIGTEPVYGVAVTSDGGRVVAGDNGKRIRV
                     WQLSDGALLRTIATGVIPMALALTPDGEAVVAGCDDYKIRVWRLSDGQLIRTFVEQHT
                     SVVKGVAVTPDGQTVVSGSLDETIKVWRLSDGARLRTIVAGGPVWSIAATP"
     assembly_gap    1573..1672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1989..2930
                     /locus_tag="P5179_10810"
     CDS             1989..2930
                     /locus_tag="P5179_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95750.1"
                     /translation="MRANTDWFRDAKWGVFMHWLAGMHRPGCTVNEWNAMVDDFDVNG
                     LANQLAEAGAGYLFFTVGQIDGFYACPNAAYDRIVGITPSKCSRRDLVSDLSDALAAK
                     GIRLLVYLPAEPPYLDAEAVAKFPWVREPKGRRQAEFQVMWEGVIRDFALRWGTKIWG
                     WWFDGCYYATDMYRHPEPPNFASFAAAAKAGNPDALVAFNQGVHTPVKPQSAFEDYTA
                     GEISDAFPVWQGYDNLPGRFVGGAQYHILSYLAGKWGYDDPRFPDEFVIGYTKHVNQH
                     EGVVTWDVPNTPQGLIREPFMNQLIALGNALGTIRHK"
     gene            2941..3768
                     /locus_tag="P5179_10815"
     CDS             2941..3768
                     /locus_tag="P5179_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS95751.1"
                     /translation="MTRYIAAYDTESPRCIKGVREIVRVHRKYSMPATFYITGTTAEG
                     NPDEYRELLDDPLFEVASHTYSHKMLRDNPFCGPAQPPEGIREQILRGKDVVEKVFKR
                     DCLGLRPGCSFEFGLKGAPDVLAVVKEGGFKYVSSMAWGKDYSLPAPLNQPFRYEEDG
                     YPDIWEIPCHGWHENLLKNHNKWGPRRITLWPPEMPEAIPTDFLKTPEEEFAVNKVFL
                     DKARNDALSFVSLIWHPWSLYAFDPEMRMLELTFSYVQSVGLAPTTYAKFCAELNAQ"
     gene            3822..>5054
                     /locus_tag="P5179_10820"
     CDS             3822..>5054
                     /locus_tag="P5179_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95752.1"
                     /translation="MDTSVSRRLLQTIDCTGEFGPDRYFSHGIASVVETDAGRYREAE
                     GVPGARFAYRFAIEHVGRPHEVVIQYPDDKRRFMCVMDGTCYDLTTGVLTGGCQPLSR
                     KMLELRQVFWPRWKDCSIVFMTWGEGEPAAAARIEVFELDDLPPMRVAGDSAYGPFRE
                     IGVQYEDPCGTCASEGALNRDEWTDRIVAYMKHTGQQSLTYPLAWYHGPTFPSDREPS
                     SQLDWIVAPDRTCYSRWTSHPVDWYQRLLQRFSDEGLTFRGSLTMLRLGSLMAGMQVD
                     EEAIHRGADTYNTVLWNGTVRAGTGDWTPMYDARFMSELAKKLDDAPEYEPLNSGQPG
                     YPPFLYGERPNPDGGGPVFNPLHPTVQTAVLGFVREIAERYAKFPAFTGISFNYFAAQ
                     MLWFGSLRAGYDDYTISLF"
BASE COUNT          951 a         1455 c         1597 g          951 t
ORIGIN      
        1 tctggcgtgg cggcaatgct ccagaccggc ccccccgcta cgattgtccg caaacgcgca
       61 ccgtccgaaa ggcgccacac tttgatagtt tcgtccaagc ttccagaaac cacggtctgt
      121 ccgtcgggtg taacggcaac gcctttcaca acactcgtat gctgttctac gaacgttcga
      181 attagctggc catccgaaag ccgccaaact ctgattttgt agtcatcgca ccccgcgacg
      241 accgcttcac catctggcgt gagagctaag gccatgggga tcacgccagt cgcgatggtt
      301 ctcaggagag caccgtcgga aagctgccac accctgattc tcttcccgtt gtcgcccgca
      361 acaactctcc caccgtcaga cgtcacggca acgccgtaaa cgggttcagt tccgatctgc
      421 ccgagcgtac tcaacaacgc gccgtctgaa atgcgccaaa ccttgactgt cccggctatt
      481 cccgcggcaa cgttctggcc atcaggggtg atggcgacgc tgctcgccga gctctccaat
      541 cccgtgagcg tccggaacga gtcgtccgcg ttcgcggcgc cgcgcaacgc aatggttgcg
      601 aagagaagag caaaaccgag ggatttcgtg cgcatggtgg ttcccccttc cccgcatggt
      661 gcggggtggc gcgatgcgtt cgcttagaaa aggcaaaggc tctcgaatat ccggcagatt
      721 gaagagcgcc gtgggcgggt cagcgcggtc gtgcggcgtc tcggacgcat gcatccacca
      781 cctgccacct gcgcacgccc cgacccgtgc gcgagcggaa aaaatttgga tggacaacat
      841 aaccggttca cgagtcaata ataccttgtt tatagcgcct gtcaaatagg actgtttttc
      901 gaacgcaccg ggcggcggag atgccgctca gaagcgcgca atttcggcgt ttctgcccgg
      961 cggcgtaccc ggattgaact ttttttgcga aaacgcttga cacgaggacg ccgcgttgcc
     1021 agtgtgtgaa tatggccggg cgaggttgta cccagacgca tcggtcgacg acgccgtcat
     1081 tctgtgcgga gcgagtaatc tccgcgttgg cgcaggccac ggtcatctcg atacagcggc
     1141 ttccgctttg cttcagccgc cactcgacga ccgtgcttgg gccgcctgtg ccgtcgcgcg
     1201 aagtcactga acggcggtgg ctggccggtc attgagcctg tcgaaatgcc gggacgctgt
     1261 atcgagatgc accggtgaac tacgccgtca ttctgtgcgg agcgaggaat ctccgcgttg
     1321 gcgcaggcca cggtcatctc gatacagcgg cttccgcttt gcttcagccg ccactcgatg
     1381 accgtacgtg ggccgcctgt gctggtcgcg ccgagccact ggacggcggt gcccggccgg
     1441 tcattgagcc tgtcgaaatg ccggcccgat gtttcgagat gcatcggtga actacggcgt
     1501 cattctgagc gcagcgaaga atctctgcgt tggcgcaggc cacggtcatc tcgatacagc
     1561 ggcttccgct ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggtgccc
     1681 ggccggtcat tgagcctgtc gaaatgccgg cccgatgttt cgagatgtac cggtgaacta
     1741 cggcgtcatt ctgagcgcag cgaagaatct ctgcgttggc acaggacacg gtcatctcga
     1801 tacagcggct tccgctttgc ttcagccgcg ggcgcggaga ccacgcccct acgctggtgt
     1861 gcgccgaacg ccactcggag accgtacgcg gcgcgccttg cgtagcccgc ttgcccctga
     1921 ggaggaatgg gcggcattct tagcactcgt ggacacagtg gattggttcc aaccgccggg
     1981 aggcacagat gcgggcaaac accgactggt tccgtgacgc gaaatggggt gtgttcatgc
     2041 actggttggc agggatgcac cgtcccggct gcaccgtgaa tgagtggaac gccatggtgg
     2101 atgatttcga cgttaacggg ctggcgaacc agcttgcgga ggcgggtgcg gggtatctgt
     2161 ttttcaccgt ggggcagatt gacggcttct acgcctgccc gaacgcggca tacgaccgga
     2221 tagtaggcat cacgccgtcg aagtgctcgc gccgggacct tgtgagcgac ctttcagacg
     2281 cgctggcggc caagggcatc cggctgctgg tgtacctgcc cgcggaacca ccgtacctcg
     2341 atgcggaggc ggtggcaaag ttcccgtggg tgcgcgaacc gaaggggcga aggcaagcgg
     2401 agtttcaggt catgtgggaa ggagtgatcc gggatttcgc gctgcgctgg ggcacgaaga
     2461 tctggggttg gtggttcgac gggtgctact acgccaccga catgtaccgc cacccggagc
     2521 cgcccaattt cgcgagtttc gcggcggcgg cgaaagcggg caatccggac gcgctggtag
     2581 cgttcaacca gggggtgcac acgccggtga agccgcagtc ggcgttcgag gactacactg
     2641 cgggtgaaat cagcgatgcg tttcctgtct ggcaggggta cgacaacctt ccgggccgtt
     2701 tcgtcggcgg ggcgcagtat cacatcctct cctaccttgc ggggaaatgg ggatacgacg
     2761 accctcgctt ccctgacgag tttgtcatcg gctacacgaa gcacgtgaat caacacgagg
     2821 gcgtggtgac gtgggacgtt cccaacacgc cgcaggggtt gatcagagaa ccattcatga
     2881 accaactgat agcgctgggc aacgcgcttg gaaccattcg acacaagtga aaggcacacc
     2941 atgacacgct acatcgcggc gtacgacacg gaatcaccac ggtgcatcaa gggagtccgt
     3001 gagattgtgc gcgtgcaccg gaagtactcg atgccggcaa cgttctacat caccggcacg
     3061 acggcggaag gcaatccgga tgaataccgg gaactgctcg acgacccgct gtttgaggtt
     3121 gcgtcgcata cctactccca caaaatgctg cgggataacc cgttctgcgg cccggcgcag
     3181 ccgccggagg ggatacggga gcagatcctg cggggcaagg acgtggttga gaaggtgttc
     3241 aaacgggatt gtctggggct tcggccggga tgcagttttg agttcggcct caagggcgcg
     3301 ccggatgtgc tggcagtggt gaaggaaggc gggttcaagt atgtgagcag catggcgtgg
     3361 ggaaaagact acagcctgcc tgcgccgctc aatcagccgt tccgctacga agaggacggg
     3421 tacccggata tctgggagat cccctgccac ggctggcatg agaacctgct caagaaccac
     3481 aacaaatggg gtccccgccg gattactctc tggccgccgg agatgccgga ggccatcccg
     3541 acggacttcc tcaaaacgcc ggaagaggaa ttcgccgtca acaaagtctt tctggacaaa
     3601 gcaaggaacg atgcgctgtc gtttgtgtcg ctcatctggc atccgtggtc gctgtatgcg
     3661 ttcgatccgg aaatgcggat gctcgagctg acgttttcgt atgtgcagtc cgtggggctt
     3721 gcaccgacca cgtacgcgaa gttctgtgcg gaactcaacg cccagtgaca ttcggctcgg
     3781 gttctggttg tgccgtcccg ccaatcacga gtggggagtg tatggatacc tccgtttcgc
     3841 gtcggctcct gcagacgatc gactgcactg gagagttcgg gcccgatcgg tatttctccc
     3901 acgggatcgc ttccgtcgtc gaaactgacg cgggacggta ccgggaggcg gaaggggtgc
     3961 ccggggcccg gttcgcgtac cggttcgcca tcgagcacgt gggacggccg catgaggtgg
     4021 tgatccagta ccccgacgac aagcgccggt tcatgtgcgt gatggacgga acctgctacg
     4081 acctcacgac aggcgtgctg acgggtggct gtcagccgtt gagcaggaaa atgctggagt
     4141 tgcggcaggt gttctggcct cggtggaagg actgcagcat cgtcttcatg acatggggcg
     4201 agggagagcc ggccgcggcg gcacgaatcg aggtatttga actggacgat ctgcccccga
     4261 tgcgagttgc gggtgattcg gcgtacgggc cgttccggga aatcggcgtg cagtatgaag
     4321 acccctgcgg aacgtgcgca agcgagggtg cgctcaacag agacgagtgg acggaccgta
     4381 tcgtggcgta catgaaacac accggccagc agagcctcac gtatccgctg gcatggtacc
     4441 acggcccgac gtttccctcc gaccgggaac cgagctcgca gttggactgg attgtcgcgc
     4501 cggatcgaac gtgctactcg cgctggacat cccatccggt ggactggtac caacgcctgc
     4561 tccagcgctt ttcggacgaa gggctcacgt ttcgcggctc gcttacgatg ttgcggctgg
     4621 gaagcctcat ggcgggcatg caggtggatg aggaggcgat ccatcgcggc gcggatacgt
     4681 acaacacggt gctgtggaac gggaccgtcc gagcgggcac gggcgactgg actccgatgt
     4741 acgacgcgcg gttcatgtcg gagctggcga agaaactcga cgacgcaccc gaatacgagc
     4801 cgctgaactc ggggcagccg ggctatccgc cgttccttta cggcgagcgc ccgaaccccg
     4861 atggcggggg gccggtgttc aatccactcc acccaacggt ccaaacggct gttctgggat
     4921 ttgtgcgaga aattgccgag cgttatgcga agtttcccgc attcaccggt atctcgttca
     4981 actacttcgc ggcacagatg ctctggttcg ggtctcttag ggccggctac gacgactaca
     5041 ctatcagcct gttc
//
LOCUS       DAOTNG010000235         5044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13770_length_5044_cov_6.291241, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000235 DAOTNG010000000
VERSION     DAOTNG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_5044_cov_6.291241"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="P5179_10825"
     CDS             complement(<1..1671)
                     /locus_tag="P5179_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRS95753.1"
                     /translation="MDHQMFTERVKKVMALAREESQRLGHDYVGTEHLLLGLIKEGEG
                     LAIAALTSLGLDPETIKQSVEDAVAQTGGQMLMGQAPFTPKAKEILERAMAEAKEMKV
                     QYVGTEHLLLALVKDRENVAAQILSALGADYKTIKEEVIALITGGSKAGQPKEKKSKT
                     PFLDHFGRDLTKLARENALDPVIGREKEIERVSQVLSRRKKNNPVLIGEPGVGKTAIA
                     EGLAQKIVAKEVPHVLENKRIVTLDMGGIVAGTKYRGQFEERIKAIMNELVQNREVVI
                     FIDELHTIVGAGSAEGTLDASNLFKPALSRGELQCIGATTLDEYRKHIEKDGALERRF
                     QTIMVDPPSVEDTVKILKGLRPKYEEHHRVKFPDATIEAAVMMAERYLHDRYQPDKSL
                     DVIDEAGSRVRLSKVSVPNEIKALEAQIAEIETKKESAVNEQNFEEAANLRDEEKALR
                     DKLALERRKWEEARENDVIEVTEEDIAYVVSSMTGIPVFRLAKNESEKLMHMEEELGK
                     RVVGQREAIAAVSRAIRRSRSGLKDPDRPIGSFVFLGPTGVGKTELGKA"
     gene            complement(1720..2799)
                     /locus_tag="P5179_10830"
     CDS             complement(1720..2799)
                     /locus_tag="P5179_10830"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRS95754.1"
                     /translation="MTLDALVRQTPMWLDASGAYADVIVSSRVRLARNLRHIPFSSQN
                     KTADEERVVDEVLSAAQHVRDLNQSTYFDMKQLEEHDRQLLVERHLISPALALRGGNG
                     GVMVDQDERLSIMVNEEDHLRLQALTAGFEPQAAWQMLDKVDTELCGRLDIAYDADLG
                     YLTACPTNVGTGLRASILIHLPGLVLVQTVDQVLNGIAQVGLTVRGFYGEGTEVVGSL
                     FQISNQITLGKSEHEILETLERVVRQIIDFEYSARQTLLRDARAQIEDKIWRSWGILA
                     NARVLSGQEFMNLASAVRLGVSTGVLKEPSLVLLNELLVRVQPSHIQRMAGSRLDSDQ
                     RDQNRAEYVRERFASTTTSSRQTRD"
     gene            complement(2796..3335)
                     /locus_tag="P5179_10835"
     CDS             complement(2796..3335)
                     /locus_tag="P5179_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRS95755.1"
                     /translation="MGKRVCERCKADDAIVRLISVKENTEKVEYLCANCARAEGVEIV
                     VGGKEAVQVSDSGETEEVEQGSREDSVMCPGCRLTYARFKERYRLGCAECFDAFRPLL
                     MPLLKKVHGAERHRGKRFARLADEDITSGTPRGTWMLELLKRRLQAAVEREEFEEAAR
                     LRDRIEAISKESNVERTAQ"
     gene            complement(3344..4108)
                     /locus_tag="P5179_10840"
     CDS             complement(3344..4108)
                     /locus_tag="P5179_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95756.1"
                     /translation="MSDDLPVASEVAVLAENLHKSYPTPEGDLPILVGVDLRVRKGEI
                     VAITGESGAGKSTLLHLLGGLDRPTSGQIIVDGVRIDNTDEGARSRFRNERVGFVFQF
                     HHLLPDFTALENVMMPCLIAGTDVAAAKAVALEMLTSVGLENRLGHRPNELSGGEQQR
                     VAVARALANKPAIVFADEPSGNLDYRHSRQLNDLLWSLARAKEVTFVIATHDRALALR
                     ADREIRLENGVARELTKAETASYSEEIGNVASACRG"
     gene            complement(4101..>5044)
                     /locus_tag="P5179_10845"
     CDS             complement(4101..>5044)
                     /locus_tag="P5179_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS95757.1"
                     /translation="SVAQTGAQIKGVDTLTIGDVSNIVRLFKEDSVNCTGRFFLGRDS
                     ANAEPGIVLGWQLAGKLQVFVGDRVYVVIPPKGPVNPMMIVQPRFAPFRVTGVFDSGF
                     YEFDATLAVMDIHEAQRLFDYGTSVGAVEVKLRDAFMADGVDSLVSQKLGGYPYTTTT
                     WVELNGGLYKWLMIEKWFAFIVLSLIILVAAFNIVSTLTMVVRDKTKEIGILKAMGTT
                     ASEISRIFLVQGLIVGFVGSISGGILGYTLCWIQDKYHVVRLPAELYQVNAFPVEMRV
                     LDFVAIGSAAIVICVLSALYPARRAANMDPVEAIRNE"
BASE COUNT         1005 a         1596 c         1258 g         1185 t
ORIGIN      
        1 tgccttcccc agttcagtct ttcccacgcc cgttggcccg agaaacacaa acgaaccgat
       61 cggccgatcg gggtctttca gtccgctgcg cgaacgccga attgcgcgag atactgccgc
      121 aattgcctcc cgctgcccca cgacgcgttt gcccagttcc tcttccatgt gcatgagttt
      181 ttcggattcg tttttcgcga gccggaaaac tggaatacct gtcatgctgc tcacaacgta
      241 ggcaatgtct tcttctgtca cctctatgac gtcgttttcg cgcgcttctt cccatttcct
      301 gcgttcaagc gccagtttgt ctcgcagtgc tttttcctcg tcgcgcaggt tggcagcctc
      361 ttcgaaattc tgctcgttaa ctgccgattc cttcttcgtc tcgatctcgg cgatctgcgc
      421 ttcgagcgct ttgatttcgt ttggaacact gaccttcgac agcctcaccc tggagcctgc
      481 ttcgtcgata acgtcgagcg atttgtccgg ctggtatcgg tcgtggagat atcgctccgc
      541 catcatcacg gcggcttcaa tcgtagcgtc ggggaatttc acgcggtggt gttcctcgta
      601 tttcggacgg aggcccttga gaatcttcac cgtgtcttcg accgaaggag gatccaccat
      661 gatcgtctga aaacgccgtt caagggcgcc atctttttct atgtgcttgc ggtactcgtc
      721 cagagtggtg gcgccgatac actggagctc accccgggaa agcgccggtt tgaacaggtt
      781 ggatgcgtca agcgttcctt ccgccgaacc tgccccgacg atcgtgtgca actcgtcaat
      841 gaaaatcacg acctcgcggt tttggacaag ttcgttcatg atcgctttga tgcgctcttc
      901 aaactgcccg cggtacttcg ttccggcaac gatgccaccc atatccagcg tcacaatccg
      961 cttgttttcc agcacgtgcg gcacttcctt cgccacaatt ttctgtgcga ggccctccgc
     1021 gatcgcggtt tttccaacac cgggttcacc gatcaggacc ggattattct tcttcctccg
     1081 gctgagaacc tgacttaccc gttcaatttc tttttcgcgc cctatgaccg gatcgagggc
     1141 attttcacgt gccagtttgg tcaaatcccg cccgaaatga tccagaaaag gcgtcttgct
     1201 tttcttctcc ttcggctgtc cggccttgct tccacccgtg atgagagcaa taacctcttc
     1261 cttgatcgtc ttgtagtccg ctcccagagc ggaaagaatc tgtgcggcga cattttcccg
     1321 gtctttgacg agggcaagca gcaggtgttc agtgccaaca tactgcactt tcatttcctt
     1381 cgcttccgcc attgcccttt ccagaatttc cttggccttg ggggtgaacg gagcctgtcc
     1441 cataagcatc tgaccgccgg tttgggcgac cgcgtcttca acagactgct ttatagtctc
     1501 cggatccagc cccaggctcg tcagcgcggc aatggccaga ccttcacctt ccttgataag
     1561 tcccaggagg aggtgctcag tccccacgta gtcatgaccc aggcgttggg actcctcccg
     1621 ggcgagcgcc atgaccttct tcacccgctc cgtaaacatc tggtgatcca ttgcgttgcc
     1681 tccaccttcc attccggtgt tatcgatttt cagtgtttct caatctcgtg tttgcctgct
     1741 ggaagtcgtc gtgctcgcga accgttctcg cacatattcg gcacggttct ggtcccgctg
     1801 gtcactgtcg agtctgcttc ctgccatgcg ctggatgtgg gaaggctgta cccgcacaag
     1861 caattcattc aaaaggacca gcgacggttc tttgagaacc ccggtgctta ctcccagtct
     1921 cacggcagat gcgagattca tgaattcctg accgctcaga acccgcgcgt tcgcgaggat
     1981 gccccaggaa cgccatatct tgtcttctat ctgcgcgcga gcatcccgca gcagggtctg
     2041 tcgcgcgctg tactcaaagt cgataatctg cctgacgacg cgttccagcg tctcgaggat
     2101 ctcgtgttcc gatttcccca gcgtgatctg atttgatatt tgaaacagac tgccgacaac
     2161 ttcggttcct tccccgtaaa agccacgaac ggtcaaccct acttgcgcga tgccgttcag
     2221 gacctgatcg accgtctgca cgagcacgag ccccggcagg tgaatgagaa tcgaagcgcg
     2281 caacccggtc cccacgttcg tcggacacgc ggtgagatac cccagatccg catcatacgc
     2341 gatatcaagc ctcccgcaga gctcggtatc aaccttgtcg agcatctgcc aggcggcctg
     2401 cggttcaaat ccagccgtaa gggcctgaag cctcaggtgg tcctcttcgt taaccatgat
     2461 gctgaggcgc tcgtcctggt ccaccatcac accgccatta cctccgcgga gggccagtgc
     2521 agggcttatc agatgccgtt ccaccagcag ttggcgatcg tgttcctcga gctgtttcat
     2581 gtcaaaatag gtgctctggt tcaggtcgcg aacgtgctgg gcggcagaaa gcacttcatc
     2641 taccacgcgt tcctcgtccg cggttttgtt ctgggacgag aaaggtatgt gccgcagatt
     2701 tcgtgcgaga cgcactctgc tcgacacgat cacgtccgca tacgcgcctg acgcatcaag
     2761 ccacatcggc gtctgccgaa ccagcgcatc gagtgtcatt gcgcagttcg ctccacattc
     2821 gattccttcg aaatcgcctc tatccggtcc cgcaacctgg cagcttcttc aaattcctcc
     2881 ctctcaaccg ctgcctgcag gcgtcttttg agaagctcaa gcatccacgt tccgcgcggc
     2941 gtacccgagg taatgtcttc gtcagccagg cgggcgaaac gtttcccccg gtgcctttcc
     3001 gcgccgtgga cctttttgag aaggggcata agcagaggcc ggaacgcatc gaaacactct
     3061 gcgcacccga gtcggtaccg ctccttgaac cgcgcatacg taagccggca accgggacac
     3121 atcaccgaat cctcccggga accctgttct acttcctcag tttctccgga gtctgacacc
     3181 tgaactgcct ccttgccgcc cacaacaatc tccactcctt ctgcacgggc gcaattcgcg
     3241 cagaggtatt caaccttttc cgtgttctct ttcaccgaga tcagacgcac aatcgcgtca
     3301 tctgctttgc accgctcaca tacgcgtttg cccatggaac ctttcagccc cgacaagctg
     3361 acgcaacatt accgatctct tcagaatagg acgccgtttc ggcttttgtc agttcccgcg
     3421 cgacaccgtt ttcaaggcgg atttcgcggt ctgccctgag tgcgagcgcg cggtcgtgtg
     3481 tggcgataac gaatgtaact tctttcgcgc gcgcaagact ccacaacagg tcgttcaact
     3541 gcctgctgtg ccgatagtcg aggttccctg atggctcatc cgcgaacact atcgcgggtt
     3601 tgtttgccag tgcgcgcgcc accgcaaccc tttgctgttc accccccgaa agctcattcg
     3661 gacggtgtcc cagacggttc tccagaccaa ctgaagtgag catctccagg gcaaccgcct
     3721 ttgcggcggc aacatcggtc ccggcgatca gacacggcat catcacattt tccagggccg
     3781 tgaaatccgg cagcaggtga tgaaactgga acacgaagcc gacccgttca ttccggaacc
     3841 gcgatctcgc tccctcgtcg gtattgtcga tgcgtacgcc atcaacaata atctgcccgg
     3901 aagtcggcct gtcgagcccg ccaagaaggt gcagcaatgt gctcttgcct gcccccgatt
     3961 caccggtgat cgcgacgatt tcccctttgc ggacgcgcag gtcaacaccg acaagaatcg
     4021 gcaaatcgcc ctccggtgtc ggatagcttt tgtgcaggtt ttcggccagg acggccactt
     4081 cggacgcaac cggcagatcg tcactcattg cgaatcgcct ccaccgggtc catattggcc
     4141 gcgcgccgcg cggggtaaag cgccgacagc acgcatatca caatcgccgc ggaaccaatc
     4201 gcgacgaaat ccagaacgcg catttcaaca gggaatgcat tcacctggta gagctccgca
     4261 ggcaaacgaa cgacgtggta cttgtcctga atccagcaaa gcgtgtatcc cagaatacct
     4321 ccggaaatac ttccaacaaa tcccacaatg agtccctgca ccagaaaaat acgcgatatc
     4381 tcggacgcag ttgtccccat tgctttcagg ataccgatct ctttcgtttt gtctcgcacc
     4441 accatcgtca acgtactaac aatgttaaat gcagccacaa ggatgatgag gctcagtacg
     4501 atgaatgcga accatttctc gatcatgagc catttgtaca acccgccgtt cagctcgacc
     4561 catgtcgtcg tagtgtaggg gtaaccgcca agtttctgcg aaacgaggct atccactccg
     4621 tccgccatga aggcgtcgcg caacttcacc tcgacagcgc ctacggaagt cccgtagtca
     4681 aacagacgtt gtgcctcatg gatgtccatt accgcaagcg ttgcgtcaaa ttcgtagaaa
     4741 cccgagtcga aaacaccggt tacgcgaaaa ggtgcgaacc tcggttgaac gatcatcatc
     4801 gggttcaccg ggcctttcgg gggaatcacc acgtagaccc gatccccgac gaacacttgg
     4861 agcttacccg cgagttgcca gcccagcacg atccccggct ctgcgttcgc tgaatctcga
     4921 ccgagaaaga agcgccctgt gcagttgaca gaatcttctt tgaacaggcg cacgatgttc
     4981 gagacatcgc cgatagtcag cgtgtcgaca cccttgatct gggcgcctgt ctgagcaacc
     5041 gagg
//
LOCUS       DAOTNG010000236         5030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13806_length_5030_cov_4.866131, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000236 DAOTNG010000000
VERSION     DAOTNG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13806_length_5030_cov_4.866131"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..379
                     /locus_tag="P5179_10850"
     CDS             224..379
                     /locus_tag="P5179_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95758.1"
                     /translation="MVGEKLLAAGVITQSQLERALMEAKKNGERVGDTVVRLGFATRD
                     QVEAALK"
     gene            420..797
                     /locus_tag="P5179_10855"
     CDS             420..797
                     /locus_tag="P5179_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95759.1"
                     /translation="MPGSVVVVDRDDEMRDLFHAVLTRAGHSVRTYSRLEELSAAPEA
                     PPDVLMVDATCAPNGDLSSLVNQISAQYSSSEVVYLVNTSESALKRQLERGRIRHFVR
                     PFQIEDLRELVNTLLERRSPARR"
     gene            803..2809
                     /gene="mutL"
                     /locus_tag="P5179_10860"
     CDS             803..2809
                     /gene="mutL"
                     /locus_tag="P5179_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRS95760.1"
                     /translation="MASPTYQTSDSEPPPRRIRVLPSQLANLIAAGEVVERPSAVLKE
                     LLENALDAGARRVSVELVAGGRRLVQVSDDGHGMNPDDAVLCFERHATSKIAAPGDLN
                     AIHTYGFRGEALPSIAAVSRTTLQTCADDSGLGTEVIIEGGQLRAVKEVGRTRGTTVT
                     VRDLFFNTPARRKFLRTDATELRHILREMTGLALTKPDLGFTLSHGGRVLLECNPVST
                     WQERVRTVFGREMLLRSIPVDKTDGTGRLYGLLGRPGDSGGGFTEQFLLVNGRSVQSR
                     VFRKAVLDGFEATTESGRQPFFVLFLELDPATVDVNVHPQKREVRFRDDRAVYDLIFS
                     AVRSAFSDTASRERGASPEPLVVPAPAEEEGPPHNADDHVSGNEAHPTKPAVSGIPSF
                     WNAPPDRPYVPATAKGADRFHRLSDGEATYLPGTGSARATFSALPDIKPSPKARAEDA
                     KAAQVALDLVSKGPGSFSASEGLGAIAASELEKTVWQFQRKYIFVNTRDGLWIIDQHV
                     AHERILYEEAMAGFAGDVPASQQLLFPTVIELTPEQDAVFDEVEEIFRSLGFGIRRLS
                     DRKIEADAYPGALHNWEDGNVIRRMIDEMRELGFKRTGLKEQIAIAYSCHAAVKSGEH
                     LPPATMRWLIERLFATSMPYVCPHGRPIIVKIETAELDQRFGRV"
     gene            2832..3308
                     /locus_tag="P5179_10865"
     CDS             2832..3308
                     /locus_tag="P5179_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRS95761.1"
                     /translation="MERVLFICTGNSCRSPMAAGIFNRLAKQRKIRDIVAESAGVFAM
                     EGLRASASAITVAHRHGINLTRHRTRPYTPEIGEQASLILTMTAEQWYCVTESTPREK
                     TFLLTNFATVDNPGIDIVDPYGGSLETYEEVFAELEREILRAFDAIVEWVEAHPTG"
     gene            3305..4114
                     /locus_tag="P5179_10870"
     CDS             3305..4114
                     /locus_tag="P5179_10870"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRS95762.1"
                     /translation="MSVCGTRRTGDQQGVPPIRLALFLCILAARTAAAPFGPGELLVF
                     DLKWQFVRGGVATMSIHPVPGDTSLWQIRTRARSVGVLDVFYTVRDTLSSTIRRSTLL
                     PVRYEKLQHEGWYHRDSTYVFDQDRNTVARFSRGRHVGVLSLPGEVHDILSSLYRVRS
                     SPLSVGSHVSVRVYEGGKLYNARVNVLRREQVTVPAGTFSCVVVEPILQSEAIFVQRG
                     RLWIWLTDDARRLPVLMESAIPVGTIRAELVSFLPTSGMSGSGTSASGSSP"
     gene            4111..4584
                     /locus_tag="P5179_10875"
     CDS             4111..4584
                     /locus_tag="P5179_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS95763.1"
                     /translation="MITALTLAACGAPRSPETAPAVGERPFSFDPAEHGPGPLPEDVE
                     ETIRSLRPPPAQVDTPAVVAAPAKRDTSWTTTNGYRIQIFTSTTRELAEAEALKARER
                     FPSDSVYILFQSPWHRVRVGDFPTRQEAEEKIVEAREKGYPDAFWVRSQIRVMNR"
BASE COUNT          963 a         1569 c         1582 g          916 t
ORIGIN      
        1 cgccgcctga gcaccccatc gtggaggaga tccgtaagct cgacctgatg aacatgacgc
       61 cgcttgaggt catgcgggcg gtggacacgt ggcagcgccg gcttgaaggg gagcgagatc
      121 gccgttcggg ttgacttgcc cggccaaaat gagctacatt tgttcacttt tgcggaatct
      181 ggattgcagg gccacgcgta ccactgggtg gaggggagag acgatggtag gagagaagct
      241 gctggctgcc ggcgtgatta cgcagtcgca actggaacgg gcgctcatgg aggccaagaa
      301 aaacggggaa cgggtgggcg acacggtagt ccgcctcgga tttgccacgc gcgatcaggt
      361 ggaagctgcc ctcaaataat cgaccggcgt cggcgctcat ttccgacgga gacacatcga
      421 tgcccggttc agtggtggtg gtggacaggg atgacgaaat gcgcgacctt ttccacgcgg
      481 tgttgacccg ggcaggccat tcggttcgca cgtattcccg tctggaggag ctttccgccg
      541 cgccggaggc gccgcccgac gtgctcatgg tggacgccac atgcgccccg aacggcgacc
      601 tgtcgtcgct cgtgaaccag atttctgcgc aatactcctc cagtgaagtc gtttaccttg
      661 tcaatacttc agagagtgcc ctcaaacgtc aactcgaacg gggaaggata cgtcatttcg
      721 tccgcccgtt tcagatcgag gacctcaggg agcttgtcaa cacgctgctt gaacgcagat
      781 cccccgcgcg gaggtgagga atatggcgtc gccgacgtat cagacgtctg attccgaacc
      841 tcctccacga cgcatccgcg tcctgccgag tcaacttgcc aatctgattg ccgccggcga
      901 ggtggtggag cgtccgtccg cagtgctcaa ggaactgctc gagaacgcgc tggacgcagg
      961 cgcccggcgg gtaagtgtgg agctggtggc cggcgggcgt cggctggttc aggtgagcga
     1021 cgacggccac ggcatgaacc ccgacgacgc cgtactgtgc ttcgagcgcc atgcgacgtc
     1081 gaaaatcgcc gcgcccgggg acctgaacgc gatccacaca tacgggttcc ggggcgaggc
     1141 gcttcccagc attgccgcgg taagccgaac cacgctccag acatgcgctg acgacagcgg
     1201 cctcggtacc gaggtgatta tcgaaggtgg ccagttgcgc gcggtgaagg aagtcggccg
     1261 cacgaggggc accaccgtaa cggtgcggga ccttttcttc aatacgcccg cgcggcgcaa
     1321 gtttcttcgc acggacgcca ccgaacttcg ccacatactg cgcgaaatga ccggcctcgc
     1381 gttgacgaaa cccgatctcg ggttcaccct ttcgcacggc gggcgcgtgt tgctggagtg
     1441 caaccccgtt tcgacctggc aggagcgtgt gcggacggtg tttggccgcg agatgctgct
     1501 gcggagtatc ccggtggaca aaacggacgg cactggccgg ttgtacggtc tgctggggcg
     1561 cccgggtgat tcgggaggcg ggttcaccga gcagttcctt ctcgtgaacg gccgttcggt
     1621 tcagtcgcgc gtgtttcgca aagcggtgct cgacggtttc gaagcaacca cagaaagcgg
     1681 cagacagcca tttttcgtgc tgtttctgga actggaccct gccacggtgg atgtcaacgt
     1741 gcacccgcag aagcgcgaag tacggtttcg ggacgaccgc gcggtgtacg acctcatctt
     1801 ctcggcggtg cgcagcgcct tctcggatac cgcgtcccgt gaacgcggcg cgtcgcccga
     1861 accgctcgtg gtgcctgctc ctgcggagga agaaggcccg cctcataatg cggacgacca
     1921 cgtttccggc aacgaagcac atcctacgaa acccgccgtg agcggcatcc cgagtttctg
     1981 gaacgcacca ccggaccgcc cgtatgttcc cgcgacggca aaaggtgccg accggtttca
     2041 tcgcctgtcg gatggcgagg cgacctacct gcccggcacc ggttccgcac gggccacgtt
     2101 ctccgcgctt cccgacatca agccgagccc gaaagcacgc gcggaggatg cgaaggcggc
     2161 ccaggtagcc ctggacctcg taagcaaagg ccccggatcg ttcagcgcct cggaaggact
     2221 gggagcgatt gcggcttcag aactcgaaaa aacggtctgg cagttccaac gaaaatacat
     2281 cttcgtcaac acgcgggacg gcctctggat cattgatcag cacgtggcgc acgagcggat
     2341 cctgtacgaa gaagcaatgg cggggtttgc cggagatgta ccggcgtcgc agcagttgct
     2401 tttccccacg gtgattgaac tcactcccga acaggacgcc gtgttcgatg aggtggagga
     2461 gatattcagg tctctgggct tcggcattcg ccgcctttcg gaccggaaaa tcgaagcaga
     2521 cgcctacccc ggagcgctcc acaactggga agacggcaat gtcatccggc gtatgatcga
     2581 cgagatgagg gaactcggct tcaaacgcac ggggttgaaa gaacagatcg caatcgcgta
     2641 ttcgtgtcat gccgcggtga aatcgggaga gcatctcccg cccgcaacga tgcgctggct
     2701 catcgaacgg ctgtttgcta cctcaatgcc ctacgtctgc ccgcacgggc ggccgatcat
     2761 cgtgaaaatc gagacggccg aactggatca gcggttcgga cgggtgtagg cacgggagca
     2821 acaggaaacg catggaacgg gtgctgttca tttgcacggg gaactcctgc cggagcccca
     2881 tggcggccgg gattttcaac cgtctcgcga aacagaggaa aattcgcgac attgtggccg
     2941 agtccgccgg ggtgtttgcg atggagggcc ttcgagcgtc cgcttcggcg attacggtgg
     3001 ctcaccggca cgggatcaac ctcacccggc accgtacccg tccgtacact ccggaaatcg
     3061 gtgaacaggc atccctgatc cttaccatga cggccgaaca gtggtactgt gtgacagaat
     3121 ccacgccgcg cgagaagacg tttctcctca ccaatttcgc cactgtcgac aaccccggta
     3181 ttgacatcgt agacccctac ggcgggtcac tggagacgta cgaagaagtg ttcgcggaac
     3241 tggagcgcga gatcctccgc gcgttcgacg ccatcgtaga atgggtggag gcccacccga
     3301 cgggatgagc gtatgcggaa cgcgtcggac cggtgaccaa caaggagtcc cccccatcag
     3361 gctcgcgttg ttcctctgca tcctcgccgc ccgcacggcg gcggctccgt tcggccccgg
     3421 cgaactcctg gtattcgacc tgaaatggca attcgtcagg ggcggggtgg ccaccatgag
     3481 catccacccc gttccgggcg acacgagcct gtggcagatc cgcacccgcg cacgttctgt
     3541 gggggtgctg gatgtgttct acaccgtccg cgacaccctg agttcgacga tccggcgatc
     3601 aacgctcctc cccgtccgct acgagaaact ccagcacgag ggctggtacc accgcgacag
     3661 cacctacgtc ttcgatcagg accggaatac cgtcgcacgg ttttcccgcg ggaggcacgt
     3721 gggcgtgctc tcgttgccgg gagaagtgca cgatatcctc tcctcgctgt accgcgtccg
     3781 cagttcgccg ctttcagtgg gttcccatgt gtctgtgcgc gtgtacgaag gcggcaaact
     3841 gtacaatgcg cgggtgaacg tgctgcgccg ggaacaggtt acggttcccg ccggcacctt
     3901 ttcctgtgtt gttgtcgaac cgatattaca atctgaggcg attttcgttc agagagggcg
     3961 actgtggatc tggcttaccg acgacgcacg aagacttccc gtgttgatgg aaagcgcgat
     4021 tccggtagga acaattcgcg cggaacttgt ttcgttcctg ccgacgtccg gcatgtctgg
     4081 ttccggcacg tcggcgtccg gttcctcgcc gtgataaccg cgctcaccct tgcggcctgc
     4141 ggcgcacccc ggtcccctga aaccgcacct gcggtgggag aacgcccctt ttccttcgac
     4201 ccggcggaac atggtcccgg cccgttgccc gaggatgtgg aagagacgat ccggtcgctg
     4261 cgccccccgc ccgcccaggt ggataccccc gcagtagttg ccgcgccggc aaaacgcgac
     4321 acgtcgtgga caacgaccaa cggctaccgc atccagatat tcacttcaac cacgcgggag
     4381 cttgcggagg cggaagcgct gaaggcgcgg gaacggttcc cctcggattc cgtctacatc
     4441 ctgttccagt cgccgtggca ccgcgttcgc gtgggggatt ttccgacgag gcaggaggcg
     4501 gaggagaaga tcgtcgaggc gcgcgagaaa ggctatcccg acgcgttctg ggtgcgcagc
     4561 cagatacgcg tgatgaacag gtaagggatg cccgccgcga gcacgagatt cttcgctttc
     4621 gcttgccgcg ttcgctcggc tcagaatgac ggtagtctcg gcggagtgca aaacgggtgc
     4681 agttctgcgg ggcgcccgca ccgcgtgagc gcagcgagaa cctccctcac cataagccag
     4741 cgggccaacg actgttccgc gaacgccacg cgcacgacag ttttcaccgg gggggcttgg
     4801 cacggcggcc ctcccgcctc actctttcac atcaccacga tgtcatcgtc ccttccggga
     4861 ggccccatgg acaccgcaac cgcgtgcatc gagtgcgtgg acgacaacct tatccgcgcc
     4921 cgtctttacc acatcgccaa agaccctata ccctgccgga cgctggtgta taccctgccc
     4981 ggccacgaga agtgcacact ccatgagaca gacgacttca tcgcgcagtc
//
LOCUS       DAOTNG010000237         5023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13841_length_5023_cov_5.588768, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000237 DAOTNG010000000
VERSION     DAOTNG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13841_length_5023_cov_5.588768"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1297)
                     /locus_tag="P5179_10880"
     CDS             complement(65..1297)
                     /locus_tag="P5179_10880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS95764.1"
                     /translation="MRWIGVLLMAATVFAGCAARTHTPRARTHTSTAPNWISEVRYQD
                     PRTQCYLGSPSIVRLPDGALLATHDYFGPGAPLNHEGQEFLTSVYRSEDNGRTWQHLQ
                     HLAGQYWSGLFVHNGAVYILGTSAHNEHIVIRRSTNGGFTWTTPVDDTTGLLFRGGIG
                     DNPPGYHCAPVPVLKAQGRIWRAFEDNDGTEVFARGFKALVISADENADLLHASSWRM
                     TNRLKFDQANRPAGWGEVEGGGMGWLEGNAVEGPDGQIWDILRVNSYPVLNKGAMVQV
                     LDGGRRMVFDPATGFIDLPGGASKFTIRRDPETGIYWMLSNDMEDGARPVRRNRLSLF
                     SSSDLRTWTKRATLMEYYGDDPAQAPKTTGFQYVDWQFDGDDIMYLVRTAYDGAHNFH
                     DSNRITFGKVEGFRALAR"
     gene            complement(1497..1976)
                     /gene="nuoE"
                     /locus_tag="P5179_10885"
     CDS             complement(1497..1976)
                     /gene="nuoE"
                     /locus_tag="P5179_10885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRS95765.1"
                     /translation="MSVQISQTTQEKIAELLTRYPVKQGALLPILHLVQDELGALSLE
                     ALDWVARQIEVSPATVYGVATFYPMFRMEPVGQHHIKVCASLPCALAGCRELIAALKE
                     KLGIEVGQTTPDGKYTLSKTECLAACTEAPAVLVNKKLHRKVTAENASEFLAGFNER"
     gene            complement(2011..3303)
                     /gene="nuoD"
                     /locus_tag="P5179_10890"
     CDS             complement(2011..3303)
                     /gene="nuoD"
                     /locus_tag="P5179_10890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRS95766.1"
                     /translation="MKPHTGRPLKFASVPYDPEAPRPPVMPDPIMSRVSKPDPDSGRT
                     IFNFGPSHPSTHGTLRLVLEVEGEMITRAWAEIGYLHTGFEKLAETLNYNQFIPLSDR
                     MNYLSAINNNVGYAHAVEELLGIEITERCKYVRTALAEMSRIADHLVCIGMQAVDIGA
                     YAPFLWGFARREDLYDLFESFCGARLTTSVTRIGGMLRDVPEDFVPGVRAFLSKLEET
                     LRETDRLLTRNRIWMDRTQGIGVLSKEDALRYCVTGPVLRGSGIDHDIRKVQPYFAYD
                     RIDFDVPIGSHGDVYDRYLVRLEEMWQSIRIIRQVLDDLPGGPINVYDNKRVLPPKEE
                     VFTTIEGMIHHFELTMPKFGFKTEVGGQIYSCTESPCGELGFYVVSDGSGNPYRLRVR
                     PPSFVNYAPIEHLVKGATISDVVAIIGSLNIIVGELDR"
     gene            complement(3367..3870)
                     /locus_tag="P5179_10895"
     CDS             complement(3367..3870)
                     /locus_tag="P5179_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRS95767.1"
                     /translation="MNETPALALQSEFPDIVRGVARQSGAREQWRVDVSKDGVLPVLT
                     YAKTRLDYCYLADITAVDYLSMPNPPEYRFALVYVLRSRNVSEPLVIRALLPENDAVV
                     ESATSLWNAADWLEREVFDLFGITFLHHPNLIRILMPENFDAHPLRKDFPTEGVGYRD
                     DFPIVTR"
     gene            complement(3867..4403)
                     /locus_tag="P5179_10900"
     CDS             complement(3867..4403)
                     /locus_tag="P5179_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRS95768.1"
                     /translation="MKEGFLVTSVKDVIRWGQKNSLWPMSFGTACCGIEIMALLGPRF
                     DLARFGAEASRFTPRQSDLLIVAGRVSIKMMPVLQRIYEQMPEPKWVISMGACASSGG
                     VFNTYTLVQGVDQFIPVDVYVPGCPPRPEALIDAVMEIQQIIDQGDVTIAQFKAFAKT
                     RNPAPGQPLLQVERGPRG"
BASE COUNT          990 a         1522 c         1490 g         1021 t
ORIGIN      
        1 cgaactgaag tgcaggccgc tgctgggcct tccatcacga agagccagca gtgtaacgaa
       61 attcttacct tgccagcgcc cgaaagcctt cgaccttccc gaacgtgata cggttcgagt
      121 cgtggaaatt gtgtgcgcca tcgtacgccg tgcggacgag gtacataatg tcatcgccgt
      181 cgaattgcca gtccacgtac tggaacccgg ttgtttttgg cgcctgtgcc gggtcgtcgc
      241 cgtagtattc catcaacgtg gcgcgctttg tccatgtgcg cagatctgac gaactgaaga
      301 gggaaagtcg attccggcgc accggtcgcg cgccgtcttc catgtcgttg gagagcatcc
      361 agtagatgcc ggtctccggg tcgcgccgga tcgtgaactt cgatgcgcca ccgggcaggt
      421 cgatgaaccc ggtggccggg tcgaacacca tgcggcgtcc tccgtccagc acctgcacca
      481 tggcgccttt gttcagcacc gggtaggagt tgacgcgcag gatgtcccaa atctgcccat
      541 ccggcccttc gaccgcgttc ccctcgagcc agcccatgcc gccgccttcc acctcccccc
      601 acccggcggg gcggttcgcc tgatcgaact tcagcctgtt ggtcatgcgc cagcttgatg
      661 cgtggagcag gtccgcgttc tcgtcagcgg aaatcaccag cgccttgaag ccgcgggcga
      721 acacttccgt accgtcgttg tcctcaaacg ctcgccagat gcggccctgc gccttcagaa
      781 ccggaaccgg cgcgcaatgg tagcccggcg gattatcgcc gatgcccccc cggaacaaca
      841 ggccggtggt gtcgtcaact ggcgtagtcc acgtgaatcc gccgttcgtg ctgcgccgga
      901 tcacgatatg ttcgttgtgc gcagatgtgc caaggatata caccgctccg ttgtgcacga
      961 acagcccgga ccagtactgg ccggccagat gttgcagatg ctgccacgtg cgcccgttgt
     1021 cctccgagcg gtacacgctc gtcaggaact cctgcccctc gtggttgagc ggcgcgccgg
     1081 ggccgaagta atcatgcgtc gccaggagcg cgccgtccgg caggcggacg atgctcgggc
     1141 tgccgagata gcactgcgtg cgcgggtcct ggtacctcac ttcactgatc cagttgggcg
     1201 cagttgaggt gtgcgtccgc gcccgcggcg tatgcgtccg cgccgcgcat ccggcgaaaa
     1261 ctgtcgccgc catgagcaaa accccgatcc atctcattgt gggatccccg ttggtggtga
     1321 aaaaaggccc gcagagcact gcataggaat ttccggagca gaaccaccag acgcaaccga
     1381 ggcgggacgg atgcagagtg gagtttcagt gggtcttcag cagggcccgc gcgtattgtt
     1441 gcttaggagg cattcgcggc ggaagtggtg cacgtcatct gcgacacgaa ggcaggctat
     1501 ctctcgttga atcctgcgag aaattcagag gcgttttccg ccgtcacctt tcggtgcagt
     1561 ttcttgttca cgagcacggc cggggcttcc gtgcaggccg cgaggcattc tgttttggag
     1621 agggtgtact tcccgtcggg tgtcgtctgc cccacctcaa ttccgagctt ctccttgagt
     1681 gctgcgatga gttcgcggca tcctgccagc gcgcagggca gactcgcaca taccttgatg
     1741 tgatgctgcc cgaccggttc catgcggaac atcgggtaaa aggtcgccac gccgtacacg
     1801 gttgcgggcg agacttcgat ctgccgggct acccagtcca aggcctcgag cgaaagagct
     1861 ccgagttcgt cctgcacgag gtgaaggatc ggcagaagcg cgccctgctt caccggatat
     1921 cgggtcagca gttccgcgat cttctcctgt gtcgtctggc tgatctgcac tgacattgag
     1981 atattcccgc gtaatctttc gcgcgccgat ttaccggtca agttccccga cgatgatgtt
     2041 caggctgccg atgatggcca ccacgtccga aatggtcgcg cctttcacga ggtgttcgat
     2101 cggggcgtag ttcacaaacg acgggggacg aacacgcaga cggtacgggt tccccgagcc
     2161 gtccgacacc acgtagaagc ccagctcacc gcagggagat tcggtgcacg agtagatctg
     2221 cccgccgacc tccgtcttga agccaaactt cggcatggtg agctcgaagt ggtggatcat
     2281 tccttctatc gtggtgaaca cttcctcctt cgggggaagc acgcgcttgt tgtcgtacac
     2341 gttgatcggc ccgccgggga ggtcgtcgag cacctgtcgt atgattcgta tgctctgcca
     2401 catctcctcg aggcgtacga ggtatctgtc gtacacatcg ccgtgcgagc ctatcggcac
     2461 gtcgaaatca atccggtcgt acgcgaaata cggttgcacc ttccggatgt catggtcaat
     2521 cccgcttccc cgcagcactg gaccggttac gcagtaacgc agggcgtctt ctttggaaag
     2581 aacaccgatt ccctgtgtgc ggtccatcca gatccggttg cgggtaagaa gcctgtcggt
     2641 ttcgcgcaac gtttcttcga gtttgctcag gaatgcgcgg actcccggca cgaaatcttc
     2701 cggaacatcg cgcaacatcc caccgatgcg ggtcacggag gtggtcaacc gtgcgccgca
     2761 gaagctctcg aacaggtcgt acagatcctc gcgccgagca aacccccaga gaaatggagc
     2821 gtacgccccg atatccaccg cctgcatccc gatgcacacc aggtggtcgg cgatgcggct
     2881 catctctgcg agggcggtgc gaacatactt gcatcgctcg gtgatctcga ttcccagcag
     2941 ctcctcaacc gcgtgggcgt acccgacgtt gttgttgatc gcggagagat aattcatccg
     3001 gtcgctgagc ggaatgaact ggttgtagtt aagcgtttcc gccagcttct cgaacccggt
     3061 gtgcagataa ccgatttccg cccatgcacg cgtgatcatt tcgccttcca cctcaagcac
     3121 gagccgcaac gtgccgtgcg tggaaggatg ggaaggaccg aagttgaaaa tcgtgcgccc
     3181 ggaatcaggg tccggcttcg agactcgact catgatcgga tccggcatga ctggagggcg
     3241 cggggcctcc ggatcatacg ggacggacgc gaacttgaga ggtctgccgg tgtgtggttt
     3301 cacgacgtgt tcctgcgcac aaatcgaacc gacggggccg gaatgcgccc gcgcgtgtcg
     3361 atcggtctat ctcgtaacga tcgggaaatc gtcccgatac ccgacgcctt ccgtgggaaa
     3421 gtccttccgc agcgggtgcg cgtcgaagtt ttccggcatc agaattcgaa tcaggttcgg
     3481 gtgatgaagg aacgtgattc caaaaaggtc aaacacttcg cgctcgagcc agtctgctgc
     3541 gttccacagc gaggttgcgg actccaccac cgcatcattt tccggcaaga gtgcccggat
     3601 caccagcggc tcggacacgt tgcgcgagcg gagaacgtac accagagcga aacggtactc
     3661 gggcggattc ggcatcgaga ggtagtctac cgcggtgatg tcggcaaggt agcaataatc
     3721 gagtctggtt ttcgcgtacg tgagaaccgg cagaacgccg tctttcgaaa cgtcaacgcg
     3781 ccattgctca cgcgcgcccg attgtcttgc aacaccgcga acgatgtccg gaaactcgga
     3841 ctgaagggca agggcaggtg tctcattcat ccgcgcggtc ctcgttcgac ctgcagcagc
     3901 ggttgcccgg gcgccggatt gcgggttttt gcgaacgctt tgaactgggc aatcgtaacg
     3961 tcaccctggt cgatgatctg ctgaatctcc atgaccgcat cgataagggc ttcggggcgc
     4021 ggggggcaac cgggcacgta cacgtccacc ggaatgaact ggtccactcc ctggaccagg
     4081 gtgtacgtat tgaatactcc cccggacgac gcacaggcgc ccatgctgat tacccacttc
     4141 ggctcgggca tctgctcata gatccgttga agcaccggca tcatcttgat ggaaacgcgt
     4201 ccggcgacga tgagcaggtc cgattgccgg ggagtgaagc gagatgcttc ggcaccgaac
     4261 cgtgcgagat caaaccgcgg gccgagcaga gccatgattt cgattccaca acacgccgtg
     4321 ccaaatgaca tcggccagag gctgtttttc tggccccacc ggatgacgtc tttgacgctc
     4381 gttacgagaa aaccttcttt caaggcgaaa cctcgcaatg gcccgcgttc gacaacggtg
     4441 gattttgcag gacgccggaa aaccccgccc cggctgttgc aaaccaaaga taggagccag
     4501 cttgcgcgcg gacaaggatc catctggctg cgcccgaaaa tgcgccgctt ctcggtcccg
     4561 aattcggccg cgaatatccg tgaagaattt tgtggaaagg ggcttgccaa aaccagatta
     4621 atgcacaata ttaaacgttt agcaacagga cggaaccagc tgttcgacgt tccagaactg
     4681 gcacagacat tgcgtacttt taccagttaa tacggcgagt ccaagagggg ctccgaaggg
     4741 aacccgcgtg ccgctcgagg tgttaatact gaatagattg ggagaataat gatccccgtg
     4801 tgatggacgg tcctcctccc ggctatcaca ccttatcccg gtcggcttcc tcccccttgg
     4861 ctgaccggga tccccttgga gggcgcaggt gtccggcaga cgcctgcgcc cttccctttt
     4921 ccccatcccg ggtcacagct tgcaaaacct gcagagtgag actattttcc catgcaggtt
     4981 acgcatatca ccaccgaaca gacgtggcac ggaggtatca tgt
//
LOCUS       DAOTNG010000238         5022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13846_length_5022_cov_5.305013, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000238 DAOTNG010000000
VERSION     DAOTNG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13846_length_5022_cov_5.305013"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /gene="dsbD"
                     /locus_tag="P5179_10905"
     CDS             <1..1590
                     /gene="dsbD"
                     /locus_tag="P5179_10905"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HRS95769.1"
                     /translation="PKFKGRIEFRTPLFLARTANPVRLTIPVEVRAQACSEEMCFFPS
                     QQVISAAITVAPRTEEALQMPRDVGSARLDSAREAALTAHAPDSASRPGSSEGFNLGS
                     LLAERGFAFAFGLIFLGGVLTSFTPCVYPLIPITVSFFASRGGAGGKKSRLRGLLLSV
                     VFVLGMATMYSLLGVLAAATGSVFGAVMANPVVIGAVALLFVAFAFSMFGAFEITLPV
                     SLQTRLSAIGGAGGLTGVFAAGLVAGIVAAPCTGPVLGAVLTYVATTGNTPFGFVALF
                     VFALGMGLLFVAIGTFSSSLMPKPGPWLEKIKSVFGIVMLVMALYFLGGVFPPLQTLW
                     LSGLRGVAISLVLTFLGLLTGAVHLRFHAGFDREGNPEAPPTIWERSRKAAGVILCVA
                     GIYGVVLTATSPPAKEAATANGVTWESDEASALARAIEQRKPILIDAYAEWCVACREL
                     DAITFADPRVQQRLRSFVTLKLDFTAITPETQKLRQKYGIVGLPTVLVLDENGVEEKE
                     KRLVGFEPPDKFLKRLDRSYP"
     gene            complement(1612..1746)
                     /gene="rpmH"
                     /locus_tag="P5179_10910"
     CDS             complement(1612..1746)
                     /gene="rpmH"
                     /locus_tag="P5179_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRS95770.1"
                     /translation="MKPTFQPHVLRRKRTHGFRARMATPGGRKVLSRRRAKGRLRLCP
                     "
     gene            complement(1841..2653)
                     /gene="panB"
                     /locus_tag="P5179_10915"
     CDS             complement(1841..2653)
                     /gene="panB"
                     /locus_tag="P5179_10915"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRS95771.1"
                     /translation="MSDSATIATEKHSSASLKDLKAHGTKITMLTAYDFPTAQFVEAA
                     GVDMALVGDSLGNAVYGFRTTRDVTMELMLPHVAAVRRGAPNTHVVADMPFRSFDTPE
                     LAVANARRFVDVGADSVKIEGALTDVVEALRDAGIEVMGHVGLLPQTAKDFKARGRTQ
                     EEADAICAGARALERAGCFSLVLEHMPLGLAQRITEELSVPTIGIGAGPHCDGQVLVI
                     HDMLGFFFPGGRFKPPFVKRYAAIGEIAVEACRRYVEEVRSGAFPDLAHSTV"
     gene            complement(2662..>5022)
                     /locus_tag="P5179_10920"
     CDS             complement(2662..>5022)
                     /locus_tag="P5179_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS95772.1"
                     /translation="VRLSRSIELPVDQHQSLVSKGDIVFLWEGGTNGTLTAVGILQSA
                     PYRFDRNSRRQRARVGVSIVLAQPLTAEECRQDQILNALAVLERSDQTNFGLNRVTKD
                     RLLSVLAAREPALAEKLNLPPNAIRPAHEPIPGPSNRDTGAENEKDGTSPVREEFDLE
                     AVHNQVREWLSDPLVPVLLRLVRSNRERLRTLLRPEVLADIEYEAFIAEFGSPGRIQW
                     AGEDVTFMEISERTGTANTEYLDGLLADGTVSTRGNLVWTQFDRDSAKRAEQLDPNSR
                     SLRLRAALAHLLAEGRSLGVRFDRMRRSFDELWPEMATGILSVIEPDEYVPFDRPALA
                     GLQVLGISRRWRNTTDDFTVYCDLVKRVCIAGNLPDLASTHVFLTYLGNRSSGADSAE
                     PADAVRQTHSEDSAARADRRSQAPAPEDAPAHLPTEGQAVFSLYQSVRRRGVVTTLAQ
                     VISLYLSVKTVPFVCFEGVPGSGKSHLIRSFAREIGGRLYSCPLTNESVGGSSDAPIR
                     FRDLLGIVDILGRRFYPDVMYRALSHAHQRSSDLVFVFIDTPEGWQEDGWLAEYTRLL
                     DLRAEHDIEGRAVSVLPSGGLLEDSDYPAEFPFPGNVVLVLSVAGHGACGEIASERAN
                     VFTLRAPDLSLEALVAGPVPEGASPTTLAAFLADHHRIQPAATPDYAALEVWNREIIH
                     MNSFLEPLGIPVGYRLRNEILRFLAYSEELSAQLPYGVKYPLETAFDHQICQRILPRV
                     LTLLEKDEQVGDVVGELLSYAQGSRGPTPRFPHAAARLQAVQRRME"
BASE COUNT          989 a         1499 c         1487 g         1047 t
ORIGIN      
        1 cccaaattca aaggccgtat agagtttcgt accccgctgt tcttggctcg gaccgcgaat
       61 cccgtaaggc tgaccatccc tgttgaggtg cgcgcgcagg catgttccga ggaaatgtgc
      121 ttcttcccgt cgcaacaggt aatttcggca gcaattacgg tggccccgag aacggaagag
      181 gcgctccaga tgcctcgcga tgtcgggtca gcgcggttgg attccgcgcg agaagccgct
      241 cttacggcgc atgcgccaga ttcagcatct cgccccggtt cgagcgaagg gttcaacctc
      301 ggttccttgt tggctgaacg agggttcgcc ttcgcgttcg gactgatctt tctgggcggg
      361 gttctcacca gtttcacgcc gtgtgtgtat ccactgatcc ctatcaccgt gagtttcttt
      421 gcgtcgagag gaggtgcggg agggaaaaag tcgcgccttc gaggtctcct gttgtccgtt
      481 gtgttcgttc ttggcatggc gacgatgtac agcctcctcg gcgtcctggc tgcggcgaca
      541 gggtccgtct ttggtgccgt gatggcgaat cccgtcgtga ttggggcggt cgcgttgttg
      601 tttgttgcgt ttgcgttctc catgttcggc gcgtttgaga tcacgctgcc tgtatcgttg
      661 cagacaaggc tctccgcgat tggtggcgcg ggcgggctca ccggtgtctt cgcagcgggt
      721 ctcgtcgccg gaatcgttgc ggcaccatgc accggtccgg ttctcggcgc agtcctgaca
      781 tacgtcgcaa caaccggcaa tacaccgttc ggattcgtgg cgttattcgt gtttgcgctc
      841 gggatgggat tgctcttcgt agcaatcggc accttttcat ccagcctcat gcccaagccc
      901 ggaccgtggc tcgagaaaat caaaagcgtg ttcggaatcg ttatgctcgt gatggcactc
      961 tatttccttg gaggggtttt cccaccgctt caaacgcttt ggttgagcgg tctccgcggc
     1021 gttgcaattt cactggttct cacgtttctc ggtctcctga caggagcagt ccaccttcgg
     1081 ttccacgccg gatttgaccg cgaagggaac cccgaggccc ccccgacgat ctgggaacga
     1141 tctcggaagg ctgcaggcgt aatactctgt gtcgcaggca tatacggggt tgttctgacg
     1201 gccacctccc ctcccgcgaa ggaagccgcg acggccaacg gcgtaacgtg ggaatctgac
     1261 gaagcctcgg ccttagcccg cgcgatagag caacggaaac caatcctgat agatgcgtac
     1321 gccgagtggt gcgtggcatg ccgggaactg gacgcgatca cgtttgccga tcctcgagtg
     1381 caacagcgct tacgttcgtt cgttaccctg aagctggatt tcacggcaat aaccccggaa
     1441 acacagaaac ttcggcagaa gtatggtatc gtcggcttgc cgacggttct ggtgctggac
     1501 gagaacggcg tggaggaaaa agaaaagcgg ctcgtgggct tcgagccgcc tgataagttc
     1561 ctgaaacgac tggaccgaag ctatccgtga cgtgccccgg cctccagtga cctacgggca
     1621 caaccggagg cggcccttgg cacgacggcg actcagaacc ttgcgcccgc cgggcgtcgc
     1681 catgcgcgca cgaaagccgt gggtccgctt cctgcgcaga acgtgcggtt ggaatgttgg
     1741 cttcatcggt atccctcatt catgtcaatg gacagcgcgg aaaaacaaac aagtctatta
     1801 atatacggat tcttgatttt cggtcaacca tggtgacgct ctatactgtg ctgtgagcaa
     1861 gatcggggaa ggctccagat cgcacttctt ccacgtagcg cctgcaggcc tccacggcga
     1921 tttcaccaat ggcggcgtac cgcttgacaa aaggaggttt gaagcggccg cccggaaaga
     1981 aaaaccccag catgtcgtgg atgacgagca cctgcccgtc acagtgaggt cccgccccta
     2041 tcccgatagt cggtaccgaa agctcttcgg tgattctctg cgccaggccc agaggcatat
     2101 gctccaacac caggctgaaa catcccgctc gttccaaagc ccgggcgcca gcacagatgg
     2161 cgtccgcctc ctcctgagtg cgccctcgcg ccttgaagtc cttcgccgtc tgaggaagca
     2221 ggccgacgtg gcccatcacc tctatccccg catcgcggag cgcctcgacc acgtccgtca
     2281 gagcgccttc aatttttacc gagtcagcac cgacgtccac aaagcgacgc gcgttcgcca
     2341 cggcgagttc aggggtgtcg aagctccgga acggcatgtc tgccacaaca tgcgtattgg
     2401 gtgctccccg ccgtaccgcg gctacgtgag gcaacatcag ttccatcgtc acgtcgcgcg
     2461 tggtgcggaa accgtacact gcgtttccca gggaatcccc caccagcgcc atatccaccc
     2521 ctgcagcctc gacgaactgc gcagtgggaa aatcgtacgc ggtcagcatg gtgatcttcg
     2581 ttccgtgcgc tttcaaatcc ttcagagaag cgcttgagtg cttctctgtg gcaattgtgg
     2641 cgctgtctga catggcgaca ttcactccat cctgcgttgc accgcctgca agcgcgcagc
     2701 ggcgtgaggg aaccgggggg taggccctcg tgagccctgc gcgtacgaga gcagttcacc
     2761 gacaacatcc ccgacctgct cgtccttttc gaggagtgtg agaacgcgcg gaagaattcg
     2821 ctggcatatc tggtgatcga acgctgtctc gagcgggtat ttgacgccgt agggcaattg
     2881 ggcgctcagc tcttcggagt aggcaaggaa tcgcagtatt tcgttacgca accggtatcc
     2941 caccggtatc cccagcggct ccagaaatga gttcatgtgg atgatttccc ggttccatac
     3001 ttccagcgcg gcgtagtcag gagtcgcagc tggctggatt cgatggtggt cggcgagaaa
     3061 ggctgcgagc gttgtcggtg aggcgccttc aggcacgggc ccggcaacga gagcctcaag
     3121 cgaaaggtcc ggcgcgcgca gcgtgaaaac attcgcgcgt tcagaagcga tttctccgca
     3181 cgccccgtgc cctgcgaccg acaaaaccag aaccacgttc cccgggaagg gaaactccgc
     3241 agggtaatca ctatcttcca gaaggcctcc agaagggaga accgaaacag cacgcccctc
     3301 gatatcgtgt tctgccctga ggtccagaag tcttgtgtat tcagccaacc acccgtcttc
     3361 ctgccaccct tcgggggtgt cgataaacac gaaaacgagg tccgaggagc gttggtgggc
     3421 gtgcgacagc gcgcggtaca tgacatcggg ataaaagcgg cggccgagaa tgtcgacaat
     3481 tccgagaagg tcgcggaagc ggatgggcgc atccgaggat ccgccgacgc tctcgtttgt
     3541 caacgggcag gagtaaagcc gtccgccgat ttcgcgcgcg aaggagcgaa tcaggtgact
     3601 cttcccgctc ccgggaactc cttcaaaaca cacgaaaggg actgttttta ccgaaagata
     3661 cagggaaatc acctgtgcca gagtcgtgac tactccgcga cggcggacgc tctggtacag
     3721 ggagaaaacc gcctgtcctt cagtcggcag atgtgctggc gcgtcctccg gagcaggagc
     3781 ctgcgaccgg cggtctgcgc gcgccgcgct gtcttcggaa tgcgtctggc gcaccgcatc
     3841 ggcaggctca gccgaatcag cgccgctgct gcgatttcca agataggtca gaaacacgtg
     3901 agtggatgcg agatcgggca aattccccgc aatgcaaacc cgtttgacaa gatcgcagta
     3961 cacggtgaag tcgtcggtgg tattcctcca tcgacgggaa atgcccagca cctgaagccc
     4021 cgcgagcgca ggtcggtcaa acgggacata ctcgtccggt tcaataacgg aaagaatgcc
     4081 ggtggccatt tccggccaca actcgtcgaa ggatcggcgc attcgatcga acctgacgcc
     4141 cagtgaccgc ccttctgcca gcaggtgcgc cagtgcagca cgcagccgca atgagcgcga
     4201 gtttgggtca agttgttccg ctctcttggc ggaatcgcga tcgaattgcg tccagacgag
     4261 gttacccctc gtcgagaccg ttccgtcggc gagcaggccg tccaggtatt ccgtgttcgc
     4321 ggtgcccgtc ctttcggaaa tctccatgaa ggtgacgtct tctccagccc attggattct
     4381 gcccggcgac ccaaattcgg cgatgaacgc ttcatattcg atgtccgcca gcacttccgg
     4441 ccgcagcagt gtccttagtc gctctctgtt tgaccgaacc aaccgcaata gaacgggtac
     4501 caggggatcc gaaagccatt ctcgtacctg attatgaact gcttccaggt caaactcctc
     4561 gcggacaggc gacgtgccgt ccttttcgtt ttccgcgccg gtgtcccgat tacttggccc
     4621 agggattggc tcgtgtgccg gcctgatcgc atttggtggg agattgagct tttctgcaag
     4681 cgcgggctca cgtgcggcca gcacggaaag caaacggtcc ttggtaactc gattcaaacc
     4741 gaagttcgtc tggtcggacc tttccaatac ggcaagagcg ttcagaatct ggtcttgccg
     4801 gcattcctcc gccgtaagcg gctgcgccag aacaatggag actccgacgc gcgctctctg
     4861 cctgcgggag tttctgtcga agcggtaggg cgcagattgg aggattccca cggctgtcag
     4921 tgtcccgttg gtcccaccct cccacaggaa cacgatatct cctttgctga caagagactg
     4981 gtgctggtct acgggaagtt cgatcgaacg agacaagcgg ac
//
LOCUS       DAOTNG010000239         4987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13955_length_4987_cov_4.849757, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000239 DAOTNG010000000
VERSION     DAOTNG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13955_length_4987_cov_4.849757"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5179_10925"
     CDS             complement(<1..730)
                     /locus_tag="P5179_10925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRS95773.1"
                     /translation="MLRLVDHDTLNAIMRRLSLAPVVSSHPLTAPANRTGSRMLVLES
                     GERWAIKFDDSPTSDGQGWWREYVGWRRARRAGIPCAEEVIVLPPEEPWNCGVCCVKQ
                     IPGVTGDLMLNSTPSLLPVALRSVGKAMAEWHKAKTLAPEELFTPFFDHLVSKERFAQ
                     SFEARAVQLAESGLVSQTLRARLIFFLDQNLHRFDENTLVPIHGDANIANMLFDRFTG
                     ALSGLLDFETLSVGPARYDLAPLYG"
     gene            complement(730..1848)
                     /gene="lpxK"
                     /locus_tag="P5179_10930"
     CDS             complement(730..1848)
                     /gene="lpxK"
                     /locus_tag="P5179_10930"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRS95774.1"
                     /translation="MRRIDEWLLNPPRTKLGKSLMNGLAAASRVYTAGVRARSWAYRS
                     GVFRKTTLNTPVLSIGNITVGGTGKTPLAQFIARHFLDTGRRPVIVSRGYRKEGATLA
                     VVSDGKRLLLSPRRSGDEPYLLARTLPGVPVIVSPNRALAGFVAVSRFAPDVVIMDDA
                     FQHMKLDRNLDIVLVDAVNPFGNEKTLPGGTLREPPPNLARADVIVVSRSDQTDRLSE
                     LRERLRSLNPSAPVVLGVHAPDGLLVHPTGDSLDLTILRNARVLAVSSIGNPWSFAES
                     VRTLGAEVLDHLRFPNHAKYRHRALSRIADAARQLRCEAIITTEKDAVRLPSHIDCGC
                     PIWVLSIRFRIVEGAASFLDLLDALVTRAKTMGMNGQA"
     gene            complement(1862..3103)
                     /locus_tag="P5179_10935"
     CDS             complement(1862..3103)
                     /locus_tag="P5179_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRS95775.1"
                     /translation="MSTRAFCYDLLWKSGLSAGSPLLLLAWATGRYEVRDRLFPKPFD
                     LPFPFWMWHGASAGEVRGLRPLWEHFGGVASADVHLSCTRSGVAMWSQFGIECTHIRL
                     FPADSAASWASMCRKQYPALVIVSETELWPGLFHAMVQRRVPVLLVSARVSQRTTTLM
                     KASRLLADIAPALFVCAQTGDDAARFSSLGVPCEQTAVCGSLKWCRGSLANRADARAA
                     MQMDGDDRVVVFGSVHREEMSHVVSAIEQIRLAGVSSRFVVAPRHMTDYDYADTILKR
                     GGLRPLRLSSLAHTGILTPESVLLVDKMGVLAQLYAGADVAVIGGAWVPLGGHNPFEA
                     TAYGLPVVYGPHMEQAGCNLLEASGQATRAHDGRMAGAIIADILRNYPGPFQSAIPDA
                     LAATVEAMARWRLPSRSKAHD"
     gene            3467..4723
                     /locus_tag="P5179_10940"
     CDS             3467..4723
                     /locus_tag="P5179_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95776.1"
                     /translation="MSGTIRVAIVGCGGITSAHLNGIRILQQSGLDDVQVTLLCSRDL
                     ENAARYNKRGDAPPPLPPIVPWRDDPLNVRDVYVTDLHPGVPAELCTDWRKAVVSPDV
                     DAVIILASVGVHHTIGCAALGAGKHVLIEKPFAITVRAARKLCEIAHERGLSLGVAES
                     LRYKRETRSARWALDNGYIGKLDMVAHVSLGSVWSPDKIVGRTPWRHHKLETGTGILM
                     DIGSHLFDKVRYVCGEFDTISGITRTLEPFRTTRDDAGHIIAQTHCDVEDTAFATAVF
                     KNGAIGTFSMSWATHGEPASLPGGTALFGDLGCIKEGELIGELEGVRVLEDLFVGRAP
                     KGLQEEWFPRGVTDAFALELHEFFRSIREGRSPETSGIEGLRDVAACYSVVESSVRGE
                     CVRFEDVANCRVETYQRAINDQLGLG"
BASE COUNT         1042 a         1451 c         1510 g          984 t
ORIGIN      
        1 ccccgtataa cggcgcgagg tcgtaccggg cagggcccac actgagcgtt tcgaagtcca
       61 acaggcccga taacgcaccg gtgaaccggt cgaaaagcat gttggcgatg ttcgcgtcgc
      121 catgaatggg gaccagcgtg ttttcgtcga acctgtgcag gttctggtcg aggaaaaaga
      181 tgagccgagc ccgcaacgtc tgtgacacga ggccgctttc cgccagttgg actgcgcgtg
      241 cttcaaacga ttgagcgaac ctttccttgg aaacgaggtg gtcgaaaaag ggagtgaaaa
      301 gttcctctgg cgcgagggtt ttcgccttgt gccattccgc catggcctta ccgaccgaac
      361 gcaaagccac cggcagcagc gagggagtgg agttcaacat cagatcgccg gtaactcccg
      421 ggatttgctt cacgcagcac accccgcagt tccatggctc ttcgggcggc agcacaatca
      481 cttcttccgc gcaggggatt cccgcgcgac gagctcgacg ccatccgacg tattcgcgcc
      541 accatccctg tccgtccgat gtcgggctgt cgtcaaattt gatggcccaa cgttcgccgg
      601 attcgagaac cagcatccgc gaacctgtgc gatttgcggg cgcagtgagc gggtgggaag
      661 aaacgaccgg cgccagcgac aaacgacgca ttatcgcgtt caacgtatcg tgatcaacaa
      721 gccgaagcat tacgcctgcc cgttcatgcc catcgttttt gcgcgcgtta cgagcgcatc
      781 cagcagatcc aggaaggatg ccgcgccttc cacgatcctg aagcggatcg agagtaccca
      841 tatcgggcat ccgcaatcga tatgcgaagg gagacgaacc gcatcctttt ccgtcgtgat
      901 gatggcttcg cagcggagct ggcgtgccgc gtcggcgatt cgcgacaacg cacggtgtct
      961 gtatttcgca tggttgggaa acctgaggtg atcaagcact tctgctccga gcgttcgtac
     1021 agactcggca aatgaccagg gatttccgat acttgacacg gccaacacgc gtgcgtttct
     1081 aagaattgtc agatcaagcg aatcgcccgt ggggtgcacc agcagcccgt cgggggcgtg
     1141 gacacccagg acgacaggag ccgatgggtt caggcttcgc agacgctccc gcaattcaga
     1201 caacctgtct gtctggtctg aacgcgacac gacgattaca tccgcgcgcg ccagatttgg
     1261 gggcggttcc cgcagggtgc ccccgggaag cgttttctcg ttaccaaacg ggttgacggc
     1321 atccaccagc acgatatcaa ggttcctgtc cagtttcatg tgctggaatg catcgtccat
     1381 gatcaccaca tcgggagcga acctgctgac cgcaacaaag cccgccagag ctcggtttgg
     1441 actcacaatt accggcacgc cgggcagagt tcgggcgaga aggtacggtt cgtcccctga
     1501 ccttcggggt gaaagcaaaa ggcgtttgcc gtccgacaca acggccagtg tggcgccttc
     1561 tttacggtag ccgcggctca cgatcacagg tctgcgcccc gtgtccagga aatgccgggc
     1621 aatgaactgg gcgagaggtg tttttccggt gccgcccacc gtgatgttgc ctatggataa
     1681 gaccggcgtg ttcagagtgg tttttcggaa cacgccactt cggtaagccc aggaacgagc
     1741 cctcactcct gcggtataca cccttgaagc cgcagccaat ccgttcatca aggacttccc
     1801 cagcttggtt cggggcgggt tcagaagcca ttcatcgatc cgtctcacct tggcacctct
     1861 gtcaatcgtg tgctttggac ctgctcggga gtcgccatct ggccatcgcc tctacggtcg
     1921 ctgccagcgc gtccgggatt gcgctctgga aaggtccagg gtagttgcgg aggatgtccg
     1981 caatgattgc gcccgccatc ctgccatcat gagcccgcgt ggcctgacca cttgcctcga
     2041 gcagattgca tcccgcctgt tccatgtgcg gcccgtacac aactggcagg ccatacgccg
     2101 tcgcttcaaa cgggttgtgt ccgcccaaag gaacccatgc cccacctata acggccacat
     2161 cagccccggc ataaagctgc gcgaggactc ccattttgtc aacgagaagc acggattccg
     2221 gtgtcaaaat ccccgtgtga gcgaggctcg acagccgaag cggacgcaaa cctccacgtt
     2281 tcaggatggt gtctgcataa tcgtaatcag tcatgtggcg gggtgcaacg acaaaacggg
     2341 acgaaacacc cgcgaggcga atctgttcga tggcggacac aacgtgagac atttcttcgc
     2401 ggtgcaccga gccgaacaca accacccggt cgtctccatc catctgcatt gccgcacggg
     2461 catccgcacg gtttgccaga cttccgcggc accatttgag cgatccgcac acggcggtct
     2521 gctcgcaggg aactcccagc gaggaaaacc gtgcagcatc gtctcctgtc tgcgcacaga
     2581 caaacaacgc cggcgcgatg tccgccagaa ggcggcttgc tttcatgagc gtcgtggttc
     2641 gttgagaaac ccgtgcgctc accagtaaga cgggtacccg gcgttgcacc attgcgtgga
     2701 acaagccggg ccatagctcc gtttcgctca cgattaccag cgcggggtac tgttttctgc
     2761 acattgatgc ccacgacgct gccgaatcag cggggaacaa gcggatgtgc gtacactcga
     2821 ttccaaattg tgaccacatg gccacccctg agcgcgtaca tgagagatgc acgtcagcac
     2881 tggccacacc gccgaagtgt tcccagagcg gcctcaatcc gcggacctct cccgcagaag
     2941 ccccgtgcca catccagaaa gggaagggga ggtcgaaggg tttcggaaac aaacggtctc
     3001 gcacttcgta tctgccggtc gcccaggcga gcagaaggag gggactcccc gccgacaaac
     3061 cggatttcca cagcaaatcg tagcaaaatg cccgcgttga cattcgaacc acctgcatga
     3121 cgggagaagg catgagaaat atacgttcgg cggacggccg acggctccac tggcacgcgg
     3181 ttcagcgtgt tgttgcttgt tcaggcgccg tcgccgccgt acttttgaaa acgaagacga
     3241 ggggcccaga ccgtctcgcg tcgttgggat catctcgaaa gtcgaacgcg gcggaccacg
     3301 cagtctgggg aaaatccccc tgttgtggcg tagagccttc gggacgagat tcgggaaaaa
     3361 aacgtcgcat gcgcggattg tctggtgacc cgccaaaggc gtcctgtgtg tctcggcgta
     3421 atctgacgcg atgtcgcgca cacacgctgg gccgggagac catactgtga gtggaacgat
     3481 tcgggttgcg attgtcggat gcgggggaat cacgtccgcg catttgaacg gtatcaggat
     3541 tctccagcaa tcagggctcg atgacgttca agtcaccctt ctgtgttcgc gcgatctcga
     3601 aaacgccgcc cggtataaca agcggggaga tgcgccgccg ccccttccgc ccatcgtgcc
     3661 gtggcgcgat gatccgctga acgttcgcga tgtctatgtc actgaccttc acccgggggt
     3721 gccggcggaa ctttgcactg attggaggaa agcggtcgtg tcgcccgacg tcgacgcggt
     3781 gatcatcctc gcgtccgttg gagtccacca cacaatcgga tgcgccgccc ttggggccgg
     3841 gaagcacgtt ctcattgaga agccttttgc catcacggtc cgcgcggcac gaaaactttg
     3901 cgaaatcgca cacgaacggg gcttgtcgct gggcgttgcg gagagtcttc ggtacaaaag
     3961 ggagacaaga tccgcccggt gggccctgga taacgggtac atcggaaaac ttgatatggt
     4021 ggcgcacgtg agcctgggat cggtgtggtc acctgacaaa atcgtcggca gaaccccgtg
     4081 gcggcaccac aagcttgaaa ccggaacggg tatcctcatg gatatcggtt cgcacttgtt
     4141 cgacaaggtg aggtacgtct gcggtgagtt tgacaccatc tcggggatca cgcgcacgct
     4201 cgaacctttc cggacaacaa gggacgacgc tggtcacata atcgcgcaaa ctcattgcga
     4261 cgtcgaggac acggctttcg caacggctgt gttcaagaat ggagccattg gaacgttcag
     4321 catgtcatgg gccacacacg gagaacctgc gtcgcttccg ggtggaaccg cgctgttcgg
     4381 cgacctcgga tgcatcaaag aaggagaact gatcggcgaa ctcgaaggag ttcgggttct
     4441 ggaggatctt ttcgttggac gtgcaccgaa aggactccag gaagaatggt tcccacgagg
     4501 cgttacggat gcgtttgcgc tggaactcca cgaattcttt cggtccatac gggagggtcg
     4561 ttctccggag acgagtggga ttgaagggtt gcgcgatgtt gccgcatgtt acagcgtcgt
     4621 ggagtcctcg gtccgcggcg aatgtgttcg ctttgaggat gtggcgaact gccgggtgga
     4681 gacctatcag agggctatca acgaccaact gggactgggg tgaacgttgt tgggaccttc
     4741 ggctgtttat ttggtgaggc acggtcagac tgagtggaat gttgagcgga agtggcaagg
     4801 cgttacggat atccctctca atgctcgagg tcgtgcgcag gctgcggcag tggcgcgcat
     4861 gttctcctcg tccgggatgc ttggcggcgt agcgatcagg cgagttgtgt cgagcgattt
     4921 gagccgtgcg aaggaaaccg cacacgagat cgctcgcgtc gcacttgttc cactggagac
     4981 gaccccg
//
LOCUS       DAOTNG010000240         4986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_13961_length_4986_cov_5.133036, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000240 DAOTNG010000000
VERSION     DAOTNG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13961_length_4986_cov_5.133036"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5179_10945"
     CDS             <1..292
                     /locus_tag="P5179_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95777.1"
                     /translation="ARTVLSQDSLYVFPHDDRITWRTLCYIAGKSTLILAPVADPDLS
                     RTMAFLARVDVEPPVVPDDPTTWYAAGVRYATLMEVLAKRLEGVFYAQVAQR"
     gene            complement(306..896)
                     /locus_tag="P5179_10950"
     CDS             complement(306..896)
                     /locus_tag="P5179_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS95778.1"
                     /translation="MNVRDFRQPEHLVSEPFLQRWSPRALTGEPITEDELNGLFEAAK
                     WAPSAFNNQPWRFLYAHRGSPEWDVFFDLLVEGNKAWAKNAGALILLVSKRTLDHNGQ
                     PSRTHSFDTGAAWMSLALEASLRGLAAHAMAGFNANKAQQVLGVPEDFAIEVMIAVGR
                     QGPPEILPPDLRERERPNTRKSVSEIARGGVWKDWR"
     gene            complement(969..1044)
                     /locus_tag="P5179_10955"
     tRNA            complement(969..1044)
                     /locus_tag="P5179_10955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1009..1011),aa:Thr,seq:cgt)
     gene            complement(1056..1391)
                     /gene="trxA"
                     /locus_tag="P5179_10960"
     CDS             complement(1056..1391)
                     /gene="trxA"
                     /locus_tag="P5179_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRS95779.1"
                     /translation="MWKMAKPVVVTDATFDQEVLKSDILTVVDFWATWCGPCRMIAPV
                     VEELAAAYDGKVKFAKVDVDENQQHAGQYGIRSIPSLLFFKGGKVVDQVVGAVPKGTL
                     ETKIKAQLG"
     gene            complement(1480..2556)
                     /gene="holA"
                     /locus_tag="P5179_10965"
     CDS             complement(1480..2556)
                     /gene="holA"
                     /locus_tag="P5179_10965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRS95780.1"
                     /translation="MQARTRYNAPLTDSADVRDSICGQGAGAYLLLGDNGVDIEETVA
                     GVVAHFTDEATRAFNCDVFHVGEAECTAEAVVNSVRSYPVLAEVRVVIVRGIEGANAA
                     FGNELVLLVENLPPAVVLVMSGEKLDERTKWASVLKKRCTVFTFAVPRNVTPWIRQRA
                     KSRGFTFDDDAAELLSDFVGTNVFRAADEIEKLTLFVQPRKTISAEDVEAVAGIARTD
                     TVYQLNDAIDGGHSERALQIAHRLQEHSMQAATIAGLILQHWMQLRAAADVQQNPDEI
                     PLKELIGANRDFVLKKLTDFARALGRDRIRRGVRLALAAESAMKSSWLADKSVGLDAL
                     ILQLSAPPGGRAPRGHSAYARGKT"
     gene            2747..>4986
                     /locus_tag="P5179_10970"
     CDS             2747..>4986
                     /locus_tag="P5179_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS95781.1"
                     /translation="MNVFMLAAVFCMLCGAADETRAAGGEWPMYRQNPHQTGRTDAVS
                     RMREAPVLRSETYLGGPVPDLAGSDETDSSWVFDFDGDGRREQLLFGTLRIRRIDDGA
                     LLWQDNITGKTFSSALSQVADFLPDSSGLEVVAFTADMGTGEGRGYMFSFHGGISNGR
                     LAWSTPNLGDMYSPEVVAGDFNNDGKMEICLAPHYKIQVLDAATGFTRYVATWDVGRN
                     YGHFAAADVDGDGIRDFVVISDFATHLSVIKNAGSSASLLWKHTYALVDGSERDVIVL
                     PNPHSVGDLDGDGTCEVTLSLFNRPVQDLKWHATAYRGADGTVAMNIPDRYIYGIRDA
                     DNDGVKELFTIECFGTTPPLFGKLQVFNWTATSGAQARLTVEHATFVRTRIGADTTWN
                     TIAAHGDETVLLSDLDGDGIEEFLTLEDTDGDFRGDRLRSWQVAAAGISQRMEYVSSA
                     PIAVVNVHGSGAGCRIAVRNLSTNALLTLDAAGNVLSEDPNDRPKGYRPTPVVSDLDG
                     DGRNEILVTSAGGDILCYDTSGPELAIRWRRSGYAMIEMPAYTLEPLSVLTDDLDGDG
                     KKEVIARNRTQQGFASLIVYNYDGTTRWEHPFPEITDGQVEAAIDNWLSGYFVGDDRI
                     RDIVVQLHARARGSGELLVLNGATGAEVWRKTQVTTATGATSLTMGGYIPAIHDVNGD
                     GIDDIVHIAYTLLAAYDGQTGNPIYPIKNLAYEIFPKWVSYATPTVADFRGTGQHQIF
                     MNSPSYA"
BASE COUNT         1035 a         1475 c         1447 g         1029 t
ORIGIN      
        1 ggcccgcacc gttctctcgc aggactcgct gtacgtgttt ccccacgacg acagaatcac
       61 gtggcggacg ctgtgttata tcgcggggaa aagcacattg atactcgctc cggtcgctga
      121 tccggatctt tcccgcacga tggcgttcct cgcgagggtt gacgtggaac ccccggtagt
      181 tccagacgat cccaccacgt ggtacgcagc gggcgtacgt tacgccacgc tgatggaggt
      241 actggcaaaa cgtctggaag gcgttttcta cgcacaggtc gcgcaaaggt gaccctgtag
      301 agccatcacc gccagtcctt ccacactccc cccctggcga tttcggacac cgacttcctc
      361 gtgttcggcc gctctctttc gcgaagatcc ggtggcaata tctccggtgg tccctgccgt
      421 ccgaccgcaa tcatgacctc aatcgcgaaa tcctctggaa ctccgagaac ctgctgggct
      481 ttgttcgcgt tgaaaccggc catggcgtgt gctgcgagcc cgcgcagaga ggcttcaagt
      541 gcgagactca tccacgccgc tcccgtgtcg aacgagtgag tgcgggaagg ctggccgttg
      601 tggtccaggg tcctttttga gacgagcaga atcagcgcgc cggcgttttt cgcccacgcc
      661 ttgttgccct ccacaaggag gtcgaagaat acgtcccact cggggctgcc tctgtgcgcg
      721 tacaaaaaac gccatggctg attattgaaa gccgaaggtg cccacttggc cgcctcgaaa
      781 agcccgttga gctcatcttc ggtgatgggc tctcccgtca gcgcacgcgg agaccacctc
      841 tggagaaacg gctcgctgac gaggtgttcc ggttgccgga aatctctcac attcatgcca
      901 cgccccctct gcagagaaaa gacgaagaag cccgaaaagg gtagttccga ttcgggcttc
      961 tttctctatg gagccaacgc gcggaatcga accgcggacc tgctcattac gagtgagctg
     1021 ctctacctgc tgagctacgt tggcctgatc ctgatttacc ccaactgcgc tttgatcttc
     1081 gtttccagcg ttccttttgg cacggcgccc acaacctgat ctacgacttt tccgcccttg
     1141 aaaaacagaa gggacggaat gctgcggatg ccgtactggc ccgcatgctg ctggttttcg
     1201 tccacgtcga ccttggcgaa cttcaccttg ccgtcgtacg ctgcggcgag ttcttccacc
     1261 acaggtgcga tcatccggca cggtccacac catgttgccc agaaatccac cacggtgagg
     1321 atgtcgcttt tcaacacctc ctggtcgaat gtggcgtccg ttacgacgac aggtttcgcc
     1381 atcttccaga tcctttcccg cgccgccacg ccgtcatcaa acgacaccgc ggtgcgtctg
     1441 catggagtaa ttaagagtac gaccgggtat ttcggtcagt caagtcttgc ctcgcgcgta
     1501 cgctgaatgg ccacgaggag cacggccccc tggtggcgct gaaagctgga gaatcagcgc
     1561 gtcgagtccc accgatttgt cggcgagcca cgagcttttc atcgcgcttt cggcggccag
     1621 agcaagcctc actccccgtc gaatgcggtc ccgtcccagc gcacgggcga agtccgtcag
     1681 cttcttgagt acaaaatccc gattcgcccc gataagttcc ttcaacggga tctcatccgg
     1741 gttctgctgc acgtcagcag ccgcccgaag ctgcatccag tgctgcagga tcaacccggc
     1801 aattgtggcc gcctgcatcg agtgctcctg caaccgatgt gcaatctgaa gggcccgttc
     1861 agagtggcca ccgtcgatcg catcgttcaa ctggtacacg gtgtcggtgc gtgcgattcc
     1921 ggctactgcc tcaacatcct ccgcactgat agtcttcctg ggctgcacga acagcgtgag
     1981 cttttcgatt tcgtcggcgg cgcggaacac gttggttccg acaaagtcac tcaacaactc
     2041 cgctgcatcg tcatcaaacg tgaacccgcg cgatttcgcg cgttgtcgga tccacggggt
     2101 cacgttgcgc ggaacggcga atgtgaagac ggtgcaacgt tttttcagaa ctgaggccca
     2161 cttcgtccgc tcatcaagct tttcgccgct catgaccaga acaactgccg gcgggaggtt
     2221 ctcaacgagc agcacgagct cgttaccaaa tgcggcgttt gcgccttcga taccacggac
     2281 aatgacgacc cggacctcgg caagcacggg ataggagcgc acggaattga caacggcttc
     2341 tgccgtgcat tcagcctctc cgacgtgaaa aacgtcgcaa ttgaacgccc gggttgcttc
     2401 gtcagtgaaa tgggcgacaa cgcccgctac cgtctcctcg atatctactc cgttgtcgcc
     2461 aagcagcagg tacgcgcccg cgccctgacc gcagatcgaa tcgcggacgt cggcggagtc
     2521 cgtaagcgga gcgttgtatc gagttctggc ctgcatggct ggtcccgtgc cgtggcattt
     2581 gacgttgacg atcacaaaaa atacgcagtg acctcgcggc gaaccacggt cggaatggac
     2641 taccgtttgg gcgcccgatc atttttcgtt aggtattctg tggcgatctg ttgcagtgcg
     2701 atcagcaaca caaccgtgaa acgatacggt tgcgggaggt gggaaaatga acgtgttcat
     2761 gcttgctgct gtattctgca tgctgtgtgg tgctgcggac gaaacgcgag ccgcaggcgg
     2821 agagtggccg atgtatcgcc agaacccgca tcagaccggg cgtacagacg cggtcagtcg
     2881 tatgcgagag gcgccggtcc ttcgttcaga aacctatctg ggcggcccgg ttcccgatct
     2941 tgcaggatct gacgaaaccg acagttcgtg ggtgtttgat ttcgacggcg acggacggcg
     3001 agagcaattg ttgttcggga ccctgaggat ccgccgaatc gacgatggcg cgctgttgtg
     3061 gcaggacaac atcaccggga agacgttctc gtcggcattg tcacaggttg ccgatttcct
     3121 tcctgatagc tccgggcttg aggtggttgc cttcaccgcg gacatgggca ccggtgaggg
     3181 gcggggatac atgttcagtt tccacggcgg gatctcgaac ggccgtctcg cgtggagtac
     3241 gccgaatctg ggcgacatgt acagcccgga agtcgtggcg ggcgacttca acaatgatgg
     3301 caaaatggag atttgcctgg ctccgcacta caaaattcag gtccttgacg cggcaacagg
     3361 tttcacgcgc tacgtcgcta cgtgggatgt cggaaggaat tacgggcact tcgcggcagc
     3421 agatgtagat ggagacggta tcagagattt cgtggtgata tctgacttcg cgactcatct
     3481 gtcggtaatc aaaaacgccg gttccagcgc atccctcctg tggaagcaca cctatgcgct
     3541 cgtggacggt agcgaacgtg acgttatagt cctccccaat cctcattccg ttggtgatct
     3601 ggatggcgat ggcacgtgcg aagtgacact cagccttttc aaccgcccgg tccaggacct
     3661 gaagtggcac gcaactgcgt accgaggtgc ggacggaacc gtcgcgatga atattcctga
     3721 tcgctacatc tacggcattc gcgacgcaga taacgacggc gtcaaagaac ttttcacgat
     3781 cgagtgcttt ggtaccactc ccccgttgtt cggcaagttg caggtgttca actggactgc
     3841 tacctccgga gcgcaggcgc gtctcacggt ggaacacgcc acattcgtgc gcacgcgaat
     3901 cggcgccgat accacatgga acacgatcgc cgcacatggc gatgaaacgg ttctcctgag
     3961 cgatctcgat ggagacggta tcgaagaatt cctgacactg gaggacaccg acggagattt
     4021 caggggtgac aggctgcgat cgtggcaggt tgccgcggct ggaatctcac agcggatgga
     4081 gtacgtctcc tccgcgccca ttgcggtcgt aaacgttcat ggctccggcg cggggtgccg
     4141 gatcgctgtg cgaaacctca gcactaatgc tcttttgaca ctggatgccg cggggaatgt
     4201 gctgagcgag gacccgaacg acaggccgaa agggtaccgg cccacccccg tcgtaagcga
     4261 cctcgatggc gatgggagaa acgagatcct cgttacgtca gcgggcgggg acatcctgtg
     4321 ctatgacaca agtggccctg aactcgccat tcggtggcgc aggtccggat acgcgatgat
     4381 cgaaatgccg gcctacacgc tcgaaccact cagtgtactg acagacgacc tcgacggaga
     4441 cggcaaaaag gaggtcattg cccgaaaccg gactcaacag gggttcgcct ccctgatagt
     4501 gtacaactac gacggaacaa ctcgatggga gcacccgttc ccggagatta ccgacggaca
     4561 ggtggaagcc gcaattgata actggctgag tgggtatttc gtcggcgatg acagaatccg
     4621 tgacatcgtg gtgcaactcc acgccagggc gcgcgggtct ggagaactcc tggttctgaa
     4681 tggagccacc ggtgcagaag tctggcggaa aacacaggta acaaccgcca caggggccac
     4741 ctcactgacg atgggtggtt acatacccgc catacacgac gtgaacggcg atggaatcga
     4801 cgacatcgtc cacatcgcgt acacgcttct tgcggcgtac gacggtcaga caggaaaccc
     4861 gatatacccg ataaagaatc tcgcctatga gatctttccc aaatgggttt cgtacgcaac
     4921 tccgactgtg gcggatttcc ggggaacagg ccagcaccag atcttcatga acagcccttc
     4981 gtatgc
//
LOCUS       DAOTNG010000241         4911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14202_length_4911_cov_5.518534, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000241 DAOTNG010000000
VERSION     DAOTNG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14202_length_4911_cov_5.518534"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..2215
                     /locus_tag="P5179_10975"
     CDS             188..2215
                     /locus_tag="P5179_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95782.1"
                     /translation="MGRERRQREPGEGLRPVPENGLTSSFPWRGAIAGFFGVAVGCVL
                     TAYSSLYLDSSLLGDDYLPIGAFSLFLGLLAINAFLRRTGWGLTRSDVHIAYILMLVS
                     AAVPVNGFAARVLPMITGLYYYATPANGWEEYFFRHVPNWIGPRDRDVARTFYEGTIT
                     GNVPWESWLKPLAVWFIPFFGVALMVLALGIFLRRQWIENERLVFPLAQVAVAVTGSG
                     NTPTIETSLIRNKLFWVAVLVPFAINALNGLHHLYPVIPNIAAMDPDLKTSLGGYLTD
                     PVQQYMYRDFYFEFHFALIGVAMMMRQEVSLSLWFFQVFFVGVIFVFMVTGLGPGQYM
                     YTPKEQFGYVMFARWTKIGAAVVVVAVILWSLRHEFVRAWRAAFGRRPVSEEGQAPLG
                     FAFWMFVAGLAAYAGWTRALGLPPGAALAMLAITLGAYIVVARIVSEGGLIWCSVAMD
                     PVLIYPTLVGTSGLSGQTITAMAYTGFVPLAARANVLPSVMDSFKIAQGSRIRPSHVI
                     ILSLASLALAFVVSLVVVLAMTYAQGGNYMPQKHYSYGPRWIFGKAASFFTDVTGPSW
                     QAIATMIAGIAIMAGMYKLHRRYLWWPFYPLGFVIAETEVMQHQWMSIAIGWAIRSLV
                     IRFGGAAGCRSLRPAAIGVIIGELTSIMFWFGVFALTGTTGKSLTHATSTW"
     gene            2388..4466
                     /locus_tag="P5179_10980"
     CDS             2388..4466
                     /locus_tag="P5179_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95783.1"
                     /translation="MAEENAGNQPEMNVGLSAIRERHRGHSVEDRTVPPQTGSPWRGL
                     TLGIVGVVILSIVTPYADAYMESSSLSATYLPTGVVLLFLLILGMNTLLRYTRFPFTQ
                     RELMLAYVTMLVPSAMPADGWSLRLVPLLIELDYYATPVNEWKILHGSNIPHWMTPHG
                     NEVVTWFFTGRPRGARIPWEPWVRPLAIWCVLAAGLYLIMLSFSIAFRKRWMDAERLQ
                     FPLAQVPLTIMGDDPAPSWVSSFFRQPLLWAGMAVPFFFHTINGLNLYFPAIPSIRLT
                     DIFFSTIFAGSQFITEPPFAEWAGVRLNFYWSIIGISYLLRSEVSLSVWAFEWFYNFE
                     MIVFQWSGYGNGQFEWSPLHTFGYSLMARYQRAGAIAAASIAYFVASRKEIGEMVRAA
                     FGMRTTRYRGGEAAIPWWSFWAFVVGMTIYFTWTAATGMKTIAAVVLLAFFFGIAITT
                     ARIVAASGLLWVYDFFVPMHGLTVLVGTARIDPKSFTNVGYVDFVALNNRAVIMPQIL
                     DGMKISRQTGIRQSHFFLGIVLGTVVATVVSMATVLWLGYTYGGANMTDFMFRGGGEW
                     LFNRVAGFQRYRVYTNWTVIGCMSAGAAFMMSLLYLHRTYLWWPLYPLGFIIGGSVAS
                     GQMWFPVMLGWLAKAIIVRFAGAGAYDRFKATALGLVLGEFLCVGMWMAIDAATGLTL
                     HRVFPVWTPQ"
     gene            4491..>4911
                     /locus_tag="P5179_10985"
     CDS             4491..>4911
                     /locus_tag="P5179_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95784.1"
                     /translation="MAQHENEPSEPVKPAEHGPDLEAVRERHEARSNMQSALAQENQT
                     IHTGAPWRGLTLGLFGVVILSVVTPYADAFLQSSGLAATYLPTGVFLLFLFALGINTA
                     LRFTRMAFTQQELMLAYVTMLVPSAIPSDGLATRLVPL"
BASE COUNT          853 a         1401 c         1493 g         1164 t
ORIGIN      
        1 aaaccgggcg cgttatttcc acggacacca gcagagtgaa agtcgttgtc atgcctacca
       61 atgaggaact ggcgattgcc ctggaaacgg cccgtctcgt tgcgcctctg aggcgatgac
      121 ctcagttccg caagaatggc gaacctgcca ctgagttcct gccacgcctg gctccggacg
      181 gactgcgatg gggcgcgaac gacgacaacg cgagccgggg gaaggccttc gacccgtacc
      241 ggaaaacggg ctcacgtcga gctttccctg gcgtggcgcg attgcaggct ttttcggcgt
      301 cgcagtcgga tgtgtgttga ccgcctactc cagcctgtac ctcgattcct ctcttctcgg
      361 cgatgactac ctgcctatcg gagcgttctc ccttttcctc ggattactcg cgatcaacgc
      421 gttcctccgg cggacaggtt gggggctgac gaggtcggac gttcatatcg cgtacatctt
      481 gatgctggtc tcagcagccg tgccggtgaa tggtttcgcg gcacgtgtcc tgccgatgat
      541 aacagggctt tactattacg ctactcctgc aaacggctgg gaggagtatt tcttccgcca
      601 cgtgccgaat tggatagggc cgcgcgaccg cgacgtggcg cgcaccttct acgagggaac
      661 gatcaccggc aacgtgccct gggaatcgtg gttgaagccg ctggccgtgt ggttcatccc
      721 gtttttcgga gtggcgctca tggttctggc gctgggtatc tttctgcggc gtcagtggat
      781 agagaacgag cgcctcgtat tcccactggc ccaggtggcg gtggcggtaa ccggttccgg
      841 gaacacgccc acgattgaaa cctccctgat acggaataag ctgttctggg ttgccgtact
      901 ggtgcctttc gccataaatg ctctcaatgg tctccaccac ctctatcccg tcattcccaa
      961 cattgcggcg atggatccag acctgaaaac gtcccttggc ggttatctga ccgacccggt
     1021 tcagcagtac atgtaccggg acttctattt tgagttccat tttgccctca tcggggtcgc
     1081 catgatgatg aggcaggaag tatcgctgag cctctggttt tttcaggtgt tcttcgtcgg
     1141 cgtcatcttc gtgttcatgg tcactggact gggaccgggg cagtatatgt ataccccgaa
     1201 ggagcaattc ggctacgtga tgtttgcccg atggacgaaa atcggagctg cggttgttgt
     1261 tgtggcggtg atcctctggt cacttcgcca tgagtttgtt cgtgcgtggc gggcggcctt
     1321 tggccgcagg ccagtttcag aggaagggca ggcgccactc gggttcgcct tctggatgtt
     1381 tgttgcagga cttgccgcgt acgcagggtg gacgagggcg ctcgggttgc caccaggagc
     1441 cgcactcgcc atgcttgcaa tcacacttgg cgcgtacatc gtggtcgccc ggattgtgtc
     1501 cgagggaggc cttatctggt gcagtgtcgc gatggacccg gtccttatct acccgacgct
     1561 cgtcgggacg tccgggctca gcgggcagac gatcaccgcg atggcgtata cgggattcgt
     1621 gccgctcgcc gcacgcgcaa atgtgcttcc gagcgtgatg gactcgttca agatcgcgca
     1681 ggggtctcga attcgcccca gccatgtaat catcctgtct ttggcgagtc tggcgctcgc
     1741 attcgtcgtg agtcttgttg tcgtgctggc tatgacgtat gcccagggcg ggaactacat
     1801 gccccagaag cactactcat acggcccgag gtggatattc ggcaaggcgg caagcttttt
     1861 caccgatgtg acgggtcctt cgtggcaggc gatcgccaca atgatcgcag gcattgccat
     1921 catggcaggg atgtacaagc tccatcgccg ttatctctgg tggccgtttt acccgttagg
     1981 ttttgtgatc gccgaaacgg aagtcatgca gcaccagtgg atgtcaatcg cgatcgggtg
     2041 ggcaatcagg tcgctcgtga tacgtttcgg aggcgccgcg ggctgtcggt ccctccgtcc
     2101 cgcggcgatc ggcgtcataa tcggggaact gaccagtatc atgttctggt tcggtgtttt
     2161 tgcgctcacg ggaaccacag ggaagtcgtt gacgcacgcg acctcaacct ggtgatcgcg
     2221 cccgcggcgg acgctgaaga cggtcttcca ccactgtcag gcgccgtcgg ctttgcgcgg
     2281 tgttcgctga atcgtacttt caaatcgctt tctttggtgg catactcgcc gctgaagcac
     2341 tcgttggcca gcttcgcagt cgctcccctc atgttaccgg atgcacgatg gccgaggaaa
     2401 acgccggcaa ccagccagag atgaacgtcg ggctttccgc aattcgcgaa aggcaccgcg
     2461 gccattcagt ggaggaccga accgttcctc cgcagaccgg ttctccatgg cgggggctga
     2521 ctctgggcat cgtcggggtg gtcattctga gcatcgtgac accgtatgct gacgcgtaca
     2581 tggagtcctc cagcctgtcg gcaacgtacc tccccaccgg cgtcgtgctt cttttcctgc
     2641 tcatactggg catgaatacc ctcctccggt acacgcgttt cccgttcacg cagcgtgagt
     2701 tgatgcttgc ctacgtcacc atgctggtgc cttcggcgat gccggcggat gggtggtcac
     2761 tgcggctcgt gccgcttctg atcgagctgg attattacgc gactccggtg aacgaatgga
     2821 aaattctcca cgggagcaac attccgcact ggatgacacc gcacgggaat gaggttgtca
     2881 cgtggttttt cacgggacga ccgcgcggag cgcgaatccc gtgggagccc tgggtgcgcc
     2941 cgttggcgat atggtgcgta ctggcggcgg gtttgtacct catcatgctc tcgttctcaa
     3001 tcgcgtttcg caagcgatgg atggacgcag agcggcttca gttccctctt gcgcaggttc
     3061 ccctgacaat catgggagac gatccggcgc cgtcgtgggt ttcttcgttt ttccggcagc
     3121 cgttgctgtg ggcgggaatg gccgtgccct tcttcttcca cacgatcaac ggtctgaacc
     3181 tgtatttccc cgcgataccg agcattcggc tgacggacat cttcttctcc accatttttg
     3241 ccggctcgca attcatcact gagcctccgt ttgccgaatg ggcaggcgtt cggctgaatt
     3301 tctactggtc gatcatcgga atcagctacc tgttgcgctc agaggtatcg ctcagcgtct
     3361 gggcgtttga atggttctac aacttcgaaa tgatcgtgtt ccagtggagc gggtacggga
     3421 acggtcagtt tgagtggtct cctttgcaca ccttcgggta ctcgttgatg gcgcggtacc
     3481 aacgtgcggg agcaatcgcc gcggcatcga tcgcgtattt tgtcgcatcc cggaaagaaa
     3541 tcggggagat ggtgcgtgcc gcattcggga tgagaactac gagataccgc ggaggcgaag
     3601 cggcgattcc ctggtggagc ttctgggcgt tcgtcgtcgg gatgacgatt tacttcacgt
     3661 ggacggccgc aacggggatg aaaacgatcg cggcggtggt cctccttgcg tttttctttg
     3721 gaatcgcaat aaccaccgcg cgcattgttg cggcaagcgg cctcctctgg gtatacgact
     3781 tttttgttcc gatgcacggt ctcacggttc ttgtgggtac cgccagaatc gacccgaaga
     3841 gcttcaccaa cgtcggatat gtggatttcg tggcattgaa taaccgcgca gtcatcatgc
     3901 cccagatact cgacggaatg aagatctcac gacaaacggg aattcggcag agccatttct
     3961 tcctcggtat cgtacttggc accgttgtcg ccacggtcgt ttcgatggcg accgtgctct
     4021 ggctcgggta cacctacggc ggcgcgaaca tgacagactt catgttccgg ggaggcggcg
     4081 agtggctgtt caaccgtgtc gcgggatttc agcggtaccg cgtctatacg aactggacag
     4141 tgatcgggtg catgagcgca ggggcggcgt tcatgatgtc gttgctttac ctccatcgca
     4201 cctatctctg gtggccgctt tacccgctcg gtttcataat cgggggatcg gtggcatccg
     4261 ggcagatgtg gttcccggtg atgctcggct ggctcgcaaa ggcgatcatc gtccgttttg
     4321 cgggcgcggg agcgtatgac cgtttcaaag caactgcact cgggctcgta ctgggggaat
     4381 ttctttgtgt gggaatgtgg atggcgatag acgccgccac ggggttgact cttcaccgcg
     4441 tgtttcctgt ctggacaccg cagtaggaca cgtgaactgg ctggatgagc atggcacaac
     4501 acgaaaatga gccttcagaa cctgtaaagc cggcagaaca cgggccggac ctcgaggccg
     4561 ttcgcgagcg ccacgaggcg cgctccaata tgcagtcggc tcttgcgcaa gagaatcaga
     4621 cgattcacac cggagctccc tggcggggat tgaccctcgg gcttttcggt gtggtgatcc
     4681 tgagtgtcgt tactccttat gctgacgcat tcctccagtc gtcgggcctc gcggcgacct
     4741 acctgcccac cggtgtgttc cttcttttcc tcttcgcgct cgggatcaat acggcgctga
     4801 gattcacccg gatggcattc acccagcagg aactgatgct ggcatacgtg acgatgctgg
     4861 tgccttcggc catcccttcc gacggactgg ccacgcgtct cgttcccctt c
//
LOCUS       DAOTNG010000242         4899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14251_length_4899_cov_4.556152, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000242 DAOTNG010000000
VERSION     DAOTNG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14251_length_4899_cov_4.556152"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="P5179_10990"
     CDS             <1..812
                     /locus_tag="P5179_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95785.1"
                     /translation="RSRGQTSLFDAGSSDVARGPAIEETTPTLPSVEPLSVQEKLEQE
                     RELLGFYVSGHPLDPHRVDIELFCTHSSEALGELGGDTPLLIAGIIRSIRRITTKAQK
                     QMAVLVCEDFSGTFEVVVFPDVYEAAQDYLIDDQIVFVRGTRSRDEPKVMALEVIPIT
                     HARMRLAKAVDVSVASEKLTDDAVGELRRIIKAHGGTLPVYIHVTTKSHGRLRFRARS
                     GAMNPTNDCLAELGKQPAVESVTLCPAVWEVPQPPRRGYGEGNGAAAAGYR"
     gene            867..2345
                     /locus_tag="P5179_10995"
     CDS             867..2345
                     /locus_tag="P5179_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRS95786.1"
                     /translation="MRSSITVRPDSAYGVVRPELYGHFAEHLGRCIYEGIWVGEESPI
                     PNDGGIRCDTVNALKRIRPPILRWPGGCFADVYHWEDGIGPRDQRPRRPNHWWEGQDT
                     NAFGTHEYLETCERLGAEPFICLNVGSGTPREALNWVEYCTYSGDTEYTRKRARNGHP
                     LPWNVRYWAVGNENWGCGGKFEARDYAREYRRFVSFLKQTAPQGEFVICGFDQQKWNL
                     DVLDELRDDLGYIDHISIHRYVSRGTGSGFTPEEYWLLMKDVSALEDVILMTSGTVQS
                     FTQYSKRFIGIALDEWGVWHRDATVDGGLSQPNTLRDAIFAASAFHMFQRHARILSMT
                     NIAQTINVLQCLLATDGDKLALTPTYYVYDLLQPHKGATAIVTEVDGPSVEVTDAGRR
                     STLPVLDASATASPDGTMTISVINRHPSEAQEGCVKVAGSTSFPNISARCLGGTSPGA
                     ENKPGATESVAPAEVSTAPVRNGEFVHTFPPCSVTVFRLSHV"
     gene            2469..3434
                     /locus_tag="P5179_11000"
     CDS             2469..3434
                     /locus_tag="P5179_11000"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95787.1"
                     /translation="MTLPDASCEPIVLAYYYIWFTRGWFDGSHTPQYKATMADIHPAI
                     GSYDSHDPDVISEHMKQCVKAKLDALAVSWFYQKRNLTGATSDDILESVMTAASDHGL
                     RTCIDMEAAGLCADRLYEALKHYLSRYSNDPRALHAQGKPVVLCWATWKLGADVWTQI
                     TKRLREQGMDAFHVASNQMDPSYFSFLDALECYTPLSPEQTVMEQYTGARAAVDAYNE
                     TPAGRTRPGRWHATVMPGFEDRLMTDRLKRDDYAHFRMRRSGYYYWETFAAAMASRPE
                     WLHVTSFNELAEHSHIEETVEDGDLYLRLTAEFATHFKAGRTAWK"
     gene            3477..4559
                     /locus_tag="P5179_11005"
     CDS             3477..4559
                     /locus_tag="P5179_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95788.1"
                     /translation="MPRGQNLGTERIDRRDADTGLLVRQITSFPTPSTHLHYETPTFT
                     PDGRRMIFLSMRVPERGAPSDLFTAGSDGKDIVQLSDDHPSGVSNPTLAKDGQSAYYT
                     HGGTAYRTHLETLETVELGHVPGASQHAYPRAMCSWDGRWYFAMAKLSNGRIGLVRWD
                     AGTGEHVVVLEADGINHPRANPGGPEIDCSVKTRRPDGSIESRKVRFHCETLKEMNAD
                     FPTGEFKTAHSCWLGATGDYQGTLQMPHRGVIVMRRGAAEPEKVAFGGPYFWHSGASN
                     DGKWIIADTNWPDEGLWLINVETKKRERICFAGASQGHPQSTHTHPNLSDDGAMAVFT
                     SDRTGMPQVYVAYIPEEMRDRLARAD"
     gene            complement(4689..>4899)
                     /locus_tag="P5179_11010"
     CDS             complement(4689..>4899)
                     /locus_tag="P5179_11010"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95789.1"
                     /translation="RSVDGVYGAGLIGAGLGGCVEAIVDDGAVENLRNALITQYFEKH
                     ERDPFIEVMAPVEGACAVELPEFQQ"
BASE COUNT         1050 a         1437 c         1469 g          943 t
ORIGIN      
        1 accgatcccg gggacagaca tctctctttg acgccgggtc ctctgacgtc gcgcgcgggc
       61 ctgcgatcga ggagacaacg ccaactcttc cttccgtgga gccgctgtcg gtccaggaaa
      121 agcttgaaca ggaaagagaa ttgctgggct tttacgtttc aggccaccct cttgacccgc
      181 atcgggttga cattgagttg ttctgcaccc acagcagcga ggcgctgggg gaactgggcg
      241 gcgacacgcc gcttctgatc gctggaatca ttcggagtat ccgccgtatc acgacgaagg
      301 cgcagaaaca gatggcggtc cttgtgtgcg aggatttctc cggcacgttt gaagtggtcg
      361 tgttcccgga cgtctacgaa gccgcacagg attacctcat agacgaccag atcgtgttcg
      421 tgcggggcac cagatcgcgc gacgaaccga aagtgatggc gctcgaggtg atcccgatca
      481 cccatgcgcg gatgcgcctg gcaaaggcag tggatgtcag cgtcgcttcg gagaagctca
      541 ctgacgacgc agtgggggaa ctccgacgta tcatcaaagc ccacggcgga acactgcccg
      601 tgtacattca cgtaaccaca aaatcccacg gcaggttgcg gttccgcgct cgatccggcg
      661 cgatgaaccc gactaacgat tgtctggcgg agctgggaaa gcagcccgcg gtggaatcgg
      721 taacgttgtg ccctgccgtg tgggaagtac cccagccgcc gcgtcgcggt tacggtgagg
      781 gcaatggcgc ggcggcggct ggctaccgct aacctttccc ggaatcagag gcgcagtaga
      841 tccactctgc gcgggagaca tactcgatga ggtcatccat cacggtgaga ccggactccg
      901 cgtacggagt cgtgcgaccg gagctgtacg gccacttcgc tgaacatctc gggcgctgca
      961 tctacgaggg gatctgggtc ggcgaggagt cccccatacc caatgacggg ggcatacggt
     1021 gcgataccgt caacgcgctc aagcggataa ggccgccgat actgcgatgg ccgggtggct
     1081 gtttcgccga tgtgtaccac tgggaggacg ggatcgggcc gcgtgatcag cgccccagac
     1141 gtccaaatca ctggtgggag gggcaggata cgaatgcgtt cggcacccac gagtatctgg
     1201 aaacgtgcga gagactggga gcggaaccgt tcatctgcct caacgtcgga agcggtaccc
     1261 cgcgcgaggc actcaattgg gtcgagtact gcacctactc cggcgatacg gaatacacac
     1321 ggaaacgagc tcgaaacggg catccactac cctggaacgt ccgttactgg gcagtcggga
     1381 acgaaaactg gggctgcggc gggaaattcg aagcacggga ttatgcacgc gaataccggc
     1441 ggtttgtgag cttcctgaag cagacggcgc cacagggaga gttcgtgatc tgcggatttg
     1501 accagcagaa atggaacctc gatgtgctgg atgaactgcg tgacgacctc gggtatatcg
     1561 accacatctc gatccacaga tacgtctctc gcggcacggg atcgggattc actccggagg
     1621 aatactggct tcttatgaaa gacgtctccg ctctggaaga cgttattctc atgacgagcg
     1681 gtactgtcca gtcattcacc cagtactcaa agcgctttat cggcatcgcg ctcgacgagt
     1741 ggggcgtatg gcatcgcgac gccacggtag acggtgggtt gtcccagccg aatacgctgc
     1801 gggatgcgat ttttgcggcg tcggcgttcc acatgtttca aaggcacgcc cgcatcctca
     1861 gcatgacaaa catcgcgcaa accatcaacg tgcttcagtg cctcctggcc actgacgggg
     1921 acaaactcgc gcttacgccg acgtactacg tctatgacct tctccaacct cacaaaggag
     1981 cgacggcaat cgtgaccgag gttgacggac cttctgtgga ggttaccgac gccggacgga
     2041 ggagtacgct cccggttctt gatgcatcgg caacagcatc ccctgacggc accatgacga
     2101 tttctgtaat caaccggcac ccttcggagg ctcaggaggg atgcgtcaaa gtggccggaa
     2161 gtacttcttt ccccaacatc tccgcccgtt gcctcggggg aacctcgccc ggcgccgaga
     2221 acaaaccggg ggcaaccgaa tcagttgctc cggcggaggt cagcacggcc ccggtgagaa
     2281 acggggaatt cgtgcacacg ttcccgccct gttccgtgac ggtgttccgt ctcagccacg
     2341 tgtaacccgc cgccggtgtg agttccggcc gcgccctatc ccgcgcggcc ggtttgccac
     2401 cgttcggtgt ctcctcctac cttcgacagg acgatgacaa gaccgaacca ttcacctgga
     2461 gacactttat gacgcttcct gacgcctctt gtgagccgat cgtgcttgcg tattactata
     2521 tctggttcac cagaggctgg tttgatggat cccacacccc gcaatacaaa gccacaatgg
     2581 cggacattca tcccgccatc gggtcgtacg attcccatga tccggacgtg atttcggagc
     2641 acatgaagca gtgcgtgaag gcgaaacttg atgcgcttgc ggtaagctgg ttttaccaga
     2701 aaaggaacct taccggcgcg acgagcgatg acatactcga atcagtaatg acagctgcct
     2761 cagatcacgg acttcgaaca tgtattgaca tggaggcggc cgggctttgc gccgaccggt
     2821 tgtatgaggc cttgaagcac tacctcagtc gttattccaa tgacccgcgc gcacttcacg
     2881 cgcagggtaa gcctgtggtg ctgtgctggg ccacatggaa actcggggcg gatgtgtgga
     2941 ctcagatcac gaaaaggctg cgagaacagg gcatggacgc gttccacgtc gcctccaacc
     3001 agatggatcc ttcctacttc tcgttcctcg atgcgctgga gtgttacacg ccgctgtcgc
     3061 cggagcagac cgtcatggag cagtacaccg gcgcacgcgc cgccgtagat gcgtataacg
     3121 aaacgcctgc cggccggacc agaccgggaa gatggcatgc cacggtgatg ccgggtttcg
     3181 aggatcggtt gatgaccgat cggctcaaac gggacgacta cgcgcatttc cgtatgcggc
     3241 ggtctgggta ctattactgg gagacttttg ccgccgccat ggcgtcgcgt ccggaatggc
     3301 tccacgtgac gagtttcaac gaacttgccg agcattcaca catagaggaa acggttgagg
     3361 atggagatct ttacctccgt cttactgcgg aatttgccac ccatttcaaa gccgggcgaa
     3421 ccgcctggaa gtgacagaac cgaagcaggt tcaaccccgc tcttgtggag aaatcgatgc
     3481 caagaggtca aaatctggga accgaacgca tagatcgccg cgacgcggac accggtctcc
     3541 tggtgcgcca gatcaccagc ttccccaccc cgagcactca tctccactac gaaacgccaa
     3601 cgttcactcc cgacggacgg cggatgatct tcctctcgat gcgcgttccg gaacgtggcg
     3661 cgcccagtga tctctttacg gcaggaagcg atgggaagga tatcgttcaa ctgtcagatg
     3721 accacccttc gggggtatcc aatccgactt tggcgaagga cgggcagtcg gcttattaca
     3781 cacacggggg aaccgcctac cgtacccacc tcgaaactct ggagaccgtg gaactgggcc
     3841 atgtacccgg cgcctcccag catgcgtacc cccgggcaat gtgttcgtgg gatggccgat
     3901 ggtactttgc gatggccaag ctgtcaaacg gcaggatcgg ccttgttcgc tgggacgcag
     3961 gcactggtga gcacgttgtc gtgctcgaag cggacggaat caaccatccc cgtgcaaacc
     4021 cgggaggtcc agagatcgat tgcagtgtga agacgcgacg cccggatggc tccattgagt
     4081 cgcgaaaggt tcgtttccac tgcgagacgc tcaaggaaat gaacgctgat ttccccaccg
     4141 gcgaattcaa gaccgcccac tcatgctggc tcggggccac gggtgattat caaggcacgc
     4201 tccagatgcc tcacaggggc gtgatcgtca tgagaagagg ggcggcggag cctgagaaag
     4261 tggcatttgg cgggccatat ttctggcatt ccggcgcttc caatgatggc aaatggatca
     4321 ttgccgacac aaactggccg gacgaaggcc tctggcttat caacgtggaa accaagaaac
     4381 gagagcggat ctgtttcgcg ggggcatcac agggtcaccc gcagagcacg cacacacacc
     4441 cgaatctctc agacgatggc gcgatggcgg tgttcacctc cgatcgcacc gggatgcctc
     4501 aggtgtatgt ggcgtatata ccggaggaaa tgagggatcg cctggcacga gccgattgaa
     4561 agccgggtga atggggcggt ccgcggacct tgaactcgac gcgttgccca gcttcctgtc
     4621 caacgggagg tgtgcggggc ggattctttt acggagccga ttcccttagt cgggttcggg
     4681 atacgcggtt actgctgaaa ctccggtagt tcgacggcac acgctccttc gaccggcgcc
     4741 atcacttcga tgaacgggtc gcgctcgtgt ttttcgaagt actgcgtaat caacgcattc
     4801 cgcagatttt caacggcacc atcatctacg attgcttcca cgcacccacc gaggccggca
     4861 ccaatgagac cggcaccgta caccccatcc actgaacga
//
LOCUS       DAOTNG010000243         4834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14470_length_4834_cov_5.251308, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000243 DAOTNG010000000
VERSION     DAOTNG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14470_length_4834_cov_5.251308"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..457
                     /gene="ndhC"
                     /locus_tag="P5179_11015"
     CDS             89..457
                     /gene="ndhC"
                     /locus_tag="P5179_11015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRS95790.1"
                     /translation="MYPSYIPLFYMLILALVAAGSMLIGVHILGPRRHTYVKNSIPYE
                     SGLDPVAEGRMRFDVKFYLVAILFVVFDLEVVYFYPWAMVFRTLLNEGMTAFYAMLVF
                     TLTLVVGLVYEWKKGALDWK"
     gene            515..1678
                     /locus_tag="P5179_11020"
     CDS             515..1678
                     /locus_tag="P5179_11020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS95791.1"
                     /translation="MPKQYQWLSTVKFQDPRTLTYLGSPSILRLPDGALLATHDYFGR
                     GCPLNHVGEEHLTSVYRSEDDGHSWWNVTHISGAFWSGLFLHRGAVYLLGTSAQYGDI
                     VIRRSDDGGFTWTHPKDDSSGLLFRGGHYHDPPNYHCAPVPVLFSSGRIYRAFEDCDP
                     LIWGKGFRAFVISADEDADLLDARTWRMTNKLPYDPEAYPPDFGAIGRDAYGWLEGNM
                     VEAPDGTLWDILRFNSVPVLNKAAMVRVVDEGTRVEFDPETGFIDLPGGLSKFTIRRD
                     PVGGRYWMLSNDMQDFPVPIRRNRLSLFSSADLRAWEKHATLMVDDHEKTPEDSVRNT
                     GFQYVDWQFDGDDIIYLVRTAYDGAHNFHDSNRIVCAKVEGFRKLVGEREVSG"
     gene            complement(1670..2749)
                     /locus_tag="P5179_11025"
     CDS             complement(1670..2749)
                     /locus_tag="P5179_11025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRS95792.1"
                     /translation="MGVRQMVRATGLERLYSVCSRAVADGEVPGGVLIVGRHEETLVE
                     LAFGNRAILPLEEPATPDTIYDWASLTKPVLTATLFMQALEEGRVHLWEPLGRWLAVP
                     AGNPLCGVTPRQLLLHISGLPAGLPLPDGGCTRGEYLNLILETGLQNAPDAEFVYSDL
                     GFHLLAAVVEKVYEESLETLAQDRILRPLGMTDTSFCVASGDTSRTAPTERANGEMLR
                     GTVHDPVARAFGGVAGHAGLFGTARDLARFCRMVIGRGQLEGSRILQSATLDRMVAPV
                     DVGSGNARALGWDVDTRYSNPRGDIFPVRGVGHTGFTGPSCWIDPPSGIFIVLMTNRL
                     HPDGHGDAVGLRRRVANVVAAALLP"
     gene            complement(2728..3528)
                     /locus_tag="P5179_11030"
     CDS             complement(2728..3528)
                     /locus_tag="P5179_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HRS95793.1"
                     /translation="MDALLDGSYADLALSCLVLVVFAFVAWRLETVTAPGAVVMTGMG
                     VAILYVGGWHWLLPIVAFFGTSVGLSRIRFGDAEPHGPRDAWQVLANGGVATVGVLLA
                     VMNLPEWRLVDSLAFYAGAIAAATADTWATEIGIRFGGPPRDVINGNSLRKGESGGIT
                     GLGTLAAALGAMMIGLIAVTIPPDQPEDWVALGHVTLAGWLGAMLDSFLGSALQTHYR
                     CRICGAETESPEHCDTATAPRRGFLSNNQVNWACTAFGAFAAWAFVRW"
     gene            complement(3486..4367)
                     /locus_tag="P5179_11035"
     CDS             complement(3486..4367)
                     /locus_tag="P5179_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRS95794.1"
                     /translation="MVELVRTLRALVMIIRPANVVLTALSVAVGASCSSAPAGITLAA
                     ACACAALIAAGGYVVNDCFDVETDRINRPYRPIPSGALSHRTAVSWAVILFAAGVSLS
                     WLLPIRFALMVMVIALALVWYAGKLKRTILWGHILVAIISGSAFVFGGMLGTRLAPAF
                     APAFLAAAFHLGREMLKAAADYPGDMEQGYDTLAVRRGVERTCRLASIPLAGVIALSP
                     IPFIIGWFGFCYLVSVIILIDGLLAYVIVRIWQSPSSETAARMATLLKWGMVAGIATV
                     WGDVWTRSLTGVMLTLR"
     gene            complement(4457..>4834)
                     /locus_tag="P5179_11040"
     CDS             complement(4457..>4834)
                     /locus_tag="P5179_11040"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS95795.1"
                     /translation="FLSAEKDSTGRYVRGRTRLFSTVCLFRDTAPPRLVVLSPSPNGT
                     IRNGLVTVSGHVEDRGSGLDPGRDPSEVRLDGKWVPAVPDASGHFAYRSTTPLPSGRH
                     TVSIKARDLAGNVRHVSFEFRVP"
BASE COUNT          995 a         1489 c         1440 g          910 t
ORIGIN      
        1 gtcacagctt gcaaaacctg cagagtgaga ctattttccc atgcaggtta cgcagatcac
       61 taccgaacag acgtggcacg gaggcaccat gtatccgtcg tatatccctt tgttttatat
      121 gcttatcctt gcgctcgtgg cagccggctc catgctgatc ggcgtgcata tactgggtcc
      181 gcggcgccat acctatgtca agaactcgat cccttacgaa tcgggtctgg accctgtggc
      241 ggaagggcgg atgcgcttcg acgtgaagtt ttatctcgtt gccatccttt tcgtggtgtt
      301 cgatcttgag gtggtctact tctatccctg ggcgatggtg ttccgaacgt tgttgaacga
      361 aggcatgacg gcgttctacg cgatgctcgt tttcacgctc acgctggtag tcgggctcgt
      421 gtacgaatgg aaaaaaggcg cgctcgactg gaaatgagtc ccccggaacg gaaactccgg
      481 ttgtgaaaca gcctcccctg aggtgaacct cccgatgccg aaacagtacc agtggctcag
      541 caccgtaaaa ttccaggacc cgcgcacctt gacctacctc ggaagcccga gcatcctccg
      601 gcttccggac ggggcactcc ttgccacgca cgactatttc ggtcgcgggt gtcctttgaa
      661 tcatgtgggc gaggaacacc tcacaagcgt ctaccgttcg gaggatgacg gtcactcgtg
      721 gtggaacgtc acccacattt cgggtgcgtt ctggagcggt ctttttcttc accgcggcgc
      781 cgtgtatctc cttggcacct ccgcgcagta cggggatatc gtgatacggc ggtctgacga
      841 cggcgggttc acctggacgc atccgaagga tgattcgagc ggcctgttgt tccgtggcgg
      901 gcattatcac gatccgccga actaccattg tgcgccggtt cccgtgctgt tttcatccgg
      961 tagaatctat cgagcgttcg aggattgcga cccgttgatc tggggcaaag ggtttcgcgc
     1021 gttcgtgatc tccgccgatg aggacgcgga ccttctggac gcccgcacat ggaggatgac
     1081 gaacaagctg ccgtacgatc ccgaggccta tccgcccgat tttggcgcga tcggccggga
     1141 cgcctacggg tggctggaag ggaatatggt cgaagcgccc gacgggacgt tgtgggacat
     1201 cctccggttc aattctgttc cagtgctcaa caaggctgcg atggtccggg tagttgacga
     1261 aggcacacgt gtggaattcg atccggaaac cgggttcatt gacctgccgg gtggcctctc
     1321 caagtttacc atccggcggg atcctgtcgg cggccgttac tggatgctct cgaacgatat
     1381 gcaggatttc ccggtgccga tccggcgcaa ccgtctctcg ctgttttcct ccgccgatct
     1441 gagggcgtgg gaaaaacatg ctaccctgat ggtggatgac catgaaaaga cgccggaaga
     1501 ttcagtgagg aacaccggct ttcagtacgt ggactggcag tttgacgggg acgacatcat
     1561 ctaccttgtc cgcacggcat atgacggcgc gcataacttt cacgattcca accggattgt
     1621 gtgtgcgaaa gtggaagggt tcaggaaact ggtgggagag agggaggttt cagggtagca
     1681 gggctgccgc gacgacattg gccacgcgtc tgcgaagccc caccgcgtcc ccgtgtccgt
     1741 ccggatggag gcggtttgtc atcaggacga tgaagatccc cgacggcgga tcgatccagc
     1801 aggagggccc ggtgaacccg gtgtgcccca ctccgcgcac cggaaagatg tccccccgcg
     1861 gattcgaata acgcgtgtcg acatcccacc cgagtgcccg cgcattaccg cttccaacgt
     1921 caacgggtgc caccatgcgg tcaagcgtgg ccgattggag tattcggctc ccctccagtt
     1981 gtccccgtcc gattaccata cggcagaagc gtgcgagatc acgtgcggtg ccgaaaaggc
     2041 ccgcgtgacc ggctacgcct ccgaatgcgc gcgcaacggg gtcgtgcacg gtgcctctca
     2101 acatctcacc gttcgctcgt tcggtaggcg ctgtgcggga ggtgtctcct gacgcgacgc
     2161 agaacgaggt gtccgtcatt ccgagcgggc ggaggatacg gtcctgagcg agcgtttcca
     2221 gcgactcctc gtacaccttt tccactaccg cggcgaggag atggaacccc agatcactgt
     2281 acacgaactc cgcgtcgggc gcgttttgca aaccggtttc gaggatcagg ttcaggtatt
     2341 caccccgggt gcaccctccg tctggaagcg ggagccctgc gggaaggcca cttatgtgaa
     2401 gcagcaactg gcgtggtgtc acgccgcaaa gtggattacc cgcaggaacc gccagccagc
     2461 gcccgagagg ttcccacagg tggacgcgcc cctcttcgag cgcctgcatg aacagggtgg
     2521 cggttaatac aggctttgtc agcgaggccc agtcgtagat tgtatcaggg gtagcaggtt
     2581 cttcgagcgg aaggatcgcc cggtttccga acgcaagttc gacaagtgtt tcctcgtgtc
     2641 ggccgacaat aaggactccc ccgggcactt ctccatcggc gacggcgcgg ctgcacacgg
     2701 aatacaggcg ttccaggccc gttgccctca ccatctgacg aacgcccacg cggcaaatgc
     2761 cccgaacgcg gtgcaggccc agttcacctg gttgttgctg aggaatcctc gcctgggtgc
     2821 ggtcgccgta tcgcagtgct ccggcgattc ggtttccgcg ccgcatatcc tgcagcggta
     2881 gtgtgtctga agcgccgagc cgagaaaact gtccagcatg gcacccagcc acccggcgag
     2941 cgtcacgtgc ccgagagcga cccagtcctc cggctgatcc gggggtattg tgaccgcgat
     3001 gagacctatc atcattgcgc caagggctgc cgccagggtc ccgagtcccg tgatacctcc
     3061 ggattcgccc ttgcgcagcg aattcccgtt gatcacgtcc cggggaggac ctccgaagcg
     3121 gataccgatc tcggtagccc acgtgtcggc ggtagctgcc gctattgctc ccgcgtaaaa
     3181 cgcgagagaa tcaacaaggc gccattccgg gaggttcatg acggcgagaa gcacgcccac
     3241 cgtcgcaacg ccgccgttgg cgagaacttg ccacgcgtcc ctcgggccgt gaggttctgc
     3301 gtcgccgaac cgtatccgcg agaggcccac cgatgtgccg aaaaacgcca cgatgggcag
     3361 cagccagtgc catcccccga catagagaat cgccacgccc ataccggtca ttacgacggc
     3421 gccgggcgcg gtgaccgtct ccagcctcca tgccacaaat gcgaaaacga ccagaacaag
     3481 acaactcaac gcaaggtcag cataactccc gtcaaggagc gcgtccatac atccccccac
     3541 acggttgcga tgcccgccac catgccccat ttcaacaatg tcgccatgcg cgcggctgtt
     3601 tcgctcgaag gcgattgcca tatccgtacg atcacgtacg caagcaaccc gtctatcagg
     3661 ataattacgc tgacaaggta acaaaagcca aaccagccga taataaacgg tatcggggag
     3721 agcgcgatca cgcctgcaag cgggatggaa gccagccggc acgtgcgttc aacgccccgg
     3781 cgaacggcga gcgtgtcgta cccctgttcc atatcgcccg ggtaatcggc cgccgctttc
     3841 agcatctccc gcccgagatg aaacgccgcc gcaagaaagg ccggcgcgaa cgctggagcg
     3901 aggcgggtcc ccagcattcc tccgaacaca aatgcgctgc cactgataat cgcgaccaga
     3961 atatggcccc acagaatcgt gcgtttgagt ttcccggcgt accacacgag cgccagagcg
     4021 ataaccatga ccatcagagc gaagcgaatc ggcaacaacc acgagagcga aacacccgcg
     4081 gcaaagagga ttaccgccca cgaaacggcg gttcgatggc tgagcgctcc gctcgggatc
     4141 ggacggtatg gtcggtttat ccggtcggtt tccacgtcga aacagtcatt caccacgtat
     4201 ccaccggcag cgatcagcgc agcacacgcg cacgcggctg cgagggtgat gcctgccggc
     4261 gcggaagaac atgaagcgcc gactgcaacg gaaagcgccg tgagaacgac gttcgccggc
     4321 cgtatgatca ttaccagagc gcgtagagtg cgaaccagtt ccaccacgga tggtgtcccg
     4381 tctcaaaaaa cctgaaaaca accgcgataa gtgcgggaaa tgtaaaaacc ccgttctccc
     4441 gacaccagtc tcctcctcac gggaccctga attcgaagct cacgtgccga acgttgccgg
     4501 cgaggtcacg cgctttgatg gagaccgtat gacggcctga gggaagcgga gttgtggatc
     4561 ggtacgcaaa atggccgctc gcatccggta cagccggaac ccatttccca tcgagacgca
     4621 cctcggaggg gtcgcggccc gggtcaagac ccgatccccg gtcctcgacg tggccagata
     4681 ccgtgacgag gccattcctg atcgttccgt tcggagacgg ggacaacacg acaagccgcg
     4741 gcggtgctgt gtcgcggaag agacacactg ttgagaacaa ccgcgtacgt ccgcgcacgt
     4801 acctgcccgt cgagtctttt tccgcgctca gaaa
//
LOCUS       DAOTNG010000244         4825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14501_length_4825_cov_5.449895, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000244 DAOTNG010000000
VERSION     DAOTNG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14501_length_4825_cov_5.449895"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="P5179_11045"
     CDS             <1..691
                     /locus_tag="P5179_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95796.1"
                     /translation="KEKAFGAKYLNNDLLETPDLALTAAYQEINRTADIVGEMHQYAM
                     EAFGKDGEAVREKLATRCSEVETLHGQFQSYYIRLAQKNLGLMQSREKQGHVTIMDDL
                     RQISNFLGVDVVNTSARLAQVGAKFSDEGARELRQFHAFTVDLHRQTAQAIRERDRAL
                     ATAVRGRKDEGEELERRLRDSHLARLDAGLQESAATSSAHMDMLTGIRQMGRHYFRIC
                     GALERFLTPPS"
     gene            complement(773..2605)
                     /locus_tag="P5179_11050"
     CDS             complement(773..2605)
                     /locus_tag="P5179_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95797.1"
                     /translation="MMRASTREVRDVHAAMVPRGPMGMRGIIEKPRHARGAIVRLLSY
                     LKPFRGVLALVFLLIVAFTLCGLLGPYLMGVAIDRYIATKDVSGLAGVAALMLGVYVV
                     SNILQAATGWIMARVSQNALKQVRNDLFSHLQSLPLSFFDRNPAGALMSRLTNDVDAV
                     NQAVSQNITSLFGSVLSLFGILVAMFLLDRWLALATLVVVPIMFAFTRFVARYTRKGF
                     RRLQGALSELNSTMEESISGHKVVKAFRRSDAAMESFKKANDDVFRAGVYANTYALLL
                     MPLTGVLGNFFVIVLAGLGGWLALRDLVSVGVIATFIMYGQNFINPLRQLANMYNSIQ
                     AALAGAERVFEILDTPAEVDAGADAPPFEEVRGDVRFEHVRFGYQPGVPVIKDMTLAA
                     KRGTTIALVGPTGAGKTTVINLLTRFYEIDEGSISIDGIDIRRVRKADLRRKLGLVLQ
                     DTFLFSDTIMENIRYGRPDAKDEECMYAARTAEAEHFILQFPQGYHTDLTERAGNLSQ
                     GQRQLLAIARAILADPSILILDEATSSVDTRTEARIQKALLRLMAGRTSFVIAHRLST
                     IRDADQVLVVKDGEIVERGTHYELLERRGFYRHLYESQFKGETI"
     gene            complement(2602..4371)
                     /locus_tag="P5179_11055"
     CDS             complement(2602..4371)
                     /locus_tag="P5179_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS95798.1"
                     /translation="MGNLRRFSSFVRPYWKQSLASLVLLTCLVAMDLSIPRLIQRIID
                     HGIYRHDERVIVVTSLIMLAISVVSAVFAVGNNVFSVRVSESVARDLREAIFRHIQSF
                     SYGNLDEQKTGQLMVRLTSDVNALKGLVHISLRIGTRSPLLMIGSLFLMVRTSPGLAL
                     SMVPLLLLTSATVGFFATRMEPLFRAVQEKLDRLNNVLQENVAGVQVVKAFVRADHER
                     ARFGDANEDLTRRSIRVMEFMSTMGPVLTLLVNTAVVVVIWVGGQRSIRGDLSIGQIV
                     AFTNYLMTTMTPLIMTAMLANTWASAIVSARRVYRVLDTPPEIRDAENAVWLPSSAEG
                     RIVFENVSFHYGGSHDGAVLEDVNLTIEPGQTVAFLGATGAGKTTLVHLIPRFYDVTS
                     GRITIDGIDIRQIRQDSLLSVMAVVPQESILFSGTIRENIRYGKPEADDAETVLAAKA
                     AQAHDFILAFENGYDTHVEERGVNLSGGQKQRIAIARALLARPKILILDDATSSVDVE
                     TETKIHDAIAAQHFARTTLIVAQRVSTVLKADKIVILDQGRISAQGTHRTLIESSSIY
                     REICESQLTGSIPVDDSRGRAPA"
BASE COUNT         1069 a         1408 c         1385 g          963 t
ORIGIN      
        1 gaaggaaaag gcgttcggtg cgaaatacct gaacaacgac ctgttggaaa ccccggatct
       61 cgcgctgacg gccgcctatc aggaaatcaa ccgcacggcc gacatcgttg gtgaaatgca
      121 tcagtatgcg atggaggcgt tcgggaaaga tggcgaggcg gttagggaga aactggcaac
      181 ccgttgctcg gaagtggaaa ccctccatgg ccagttccag agctactaca tccgcctcgc
      241 gcagaaaaac ctcggtctca tgcagtcgag ggaaaagcag gggcatgtca ctatcatgga
      301 tgatctgagg cagatctcca actttctcgg cgtggacgtg gtcaacacgt cggcacgcct
      361 cgcgcaggtt ggtgcgaagt tctcagacga aggtgcgcgt gaactccgcc aattccatgc
      421 attcacagtg gacctgcatc ggcagaccgc ccaggccatc agggaaagag accgcgcgct
      481 ggccactgcc gtcagaggtc ggaaagacga aggcgaagaa ctggaaagac gtttgcggga
      541 tagccacctc gcgcgtctgg acgcggggct tcaggagtcc gccgccacca gcagcgccca
      601 catggatatg ctgacgggaa tccgccagat gggacgacac tatttccgca tctgcggcgc
      661 tcttgagcga ttcctgacgc cgccgtcgta acggagcgaa tcggaatcgg acacggcaaa
      721 tgcgtaagtt ggtccgcaga tggcggccgg atgcccggcc gccatctgca tttcaaatcg
      781 tttcaccctt gaactggctt tcatacaggt ggcggtagaa accgcgccgc tccagcagtt
      841 cataatgggt tccgcgttcg actatttcgc cgtcttttac cacaagaacc tggtctgcgt
      901 cacgaatcgt gctcaagcgg tgagcgatga caaagctcgt tcgtcccgcc atgagccgaa
      961 ggagtgcctt ctgtatccgt gcctcggtgc gggtgtccac gctggacgtt gcctcgtcca
     1021 gaataagaat cgaagggtct gcgaggatgg cgcgcgcgat cgcaaggagc tggcgctgcc
     1081 cctgacttag gtttcccgcg cgttcggtaa ggtcggtgtg atacccctgc gggaactgaa
     1141 gaatgaaatg ctccgcctct gccgtccggg cggcgtacat gcactcctca tccttcgcgt
     1201 ccggcctgcc gtaacggata ttctccatga tcgtatccga aaagaggaac gtatcctgca
     1261 gcaccaaccc cagtttgcgc cgaagatcag ctttgcgcac tctacgaatg tcaatgccat
     1321 ctatggagat gcttccctcg tcgatctcgt agaaccgggt aagaagattg ataacagtgg
     1381 ttttgcctgc tcccgtcggt ccgaccaggg cgatggttgt ccctcgtttt gccgcgagtg
     1441 tcatatcctt gataaccgga acgcccggct gatacccgaa acggacgtgt tcaaaacgga
     1501 cgtcaccacg gacctcctca aacgggggcg catcggctcc agcatctact tctgccggcg
     1561 tatcgaggat ttcgaaaacc cgttcggccc ctgcaagcgc tgcctgaatc gaattgtaca
     1621 tgttggcgag ctgccgcaga gggttgatga aattctgccc gtacatgatg aacgttgcga
     1681 tgactccgac actgaccaga tcccgcaggg caagccaacc gcctaacccc gccagcacga
     1741 tgacgaagaa atttccgagg actcccgtaa gaggcatgag caacaacgcg taggtgttcg
     1801 cgtaaactcc ggcacggaac acgtcgtcgt ttgccttttt gaatgactcc atcgctgcgt
     1861 cgctgcgacg gaatgccttg accaccttgt ggccgctgat ggattcctcc atcgtgctgt
     1921 taagctcgga gagcgcacct tgcagcctcc ggaacccttt ccgggtgtac cgcgcaacaa
     1981 aacgggtgaa cgcgaacatg atcggcacga cgaccagcgt ggccagcgca agccaccggt
     2041 cgagaagaaa catggcgacg aggattccga agagggacaa cacactcccg aacagggagg
     2101 taatgttctg cgagaccgcc tggttcaccg cgtccacgtc gtttgtcaac cggctcatga
     2161 gtgcgccggc ggggttgcgg tcaaaaaagc tcaggggcag gctctgaagg tgcgaaaaca
     2221 ggtcgtttcg gacctgtttc agcgcgttct gagaaacgcg tgccatgatc caccctgtcg
     2281 cagcctgcag gatgttggaa acgacgtaca ctcccagcat caacgctgcg acgccggcaa
     2341 gaccggatac atccttcgta gcgatatagc ggtctatcgc cacccccatc agatacggcc
     2401 cgagaagccc gcacagcgtg aacgcaacga tgagaaggaa gacgagcgcc agaacgccgc
     2461 gaaacggttt caggtaggac aggagtctca ctatcgctcc gcgggcatgc cggggctttt
     2521 cgatgatgcc tctcatgccc atcggcccgc gcggtaccat ggcggcgtgg acatcgcgaa
     2581 cttcgcgagt tgaggcgcgc atcatgccgg cgccctccct cggctgtcgt caactggaat
     2641 gctgcccgtc agttgggatt cgcagatttc gcgatagatc gagctggatt cgatgagtgt
     2701 ccgatgggtt ccctgtgcgg agattctgcc ctgatcgaga atgacgattt tgtccgcttt
     2761 caaaacagtg cttacgcgtt gcgcgacaat gagcgtggtg cgagcgaagt gctgcgctgc
     2821 gatcgcgtcg tgaatcttcg tttccgtctc aacatccacg gaactcgtcg cgtcgtcgag
     2881 aatcaggatt ttcggacgcg cgagaagtgc ccgggcgatg gcaatccgtt gcttctgccc
     2941 tccggaaaga ttcacgccac gttcctcaac atgggtgtcg taaccgtttt cgaaggcgag
     3001 aatgaaatcg tgggcctggg cggcctttgc ggcaagcaca gtctccgcgt cgtcggcctc
     3061 gggcttgccg taccgtatat tctcgcggat cgttccggaa aacaggatgc tctcctgcgg
     3121 gacgaccgcc atcacggaaa ggagcgaatc ctggcggatt tgccggatgt caatgccgtc
     3181 aattgtgatc cggccggaag tgacatcgta gaaacgggga atcaggtgta ccagggttgt
     3241 tttcccggct cccgttgccc ccaggaacgc cacggtctgt ccaggttcga tcgtgagatt
     3301 gacatcctcg agaaccgcgc cgtcatggct gccgccgtaa tggaacgaaa cgttttcgaa
     3361 gacgatccgg ccctcggccg aggacggcaa ccaaaccgcg ttttcggcgt ctcttatttc
     3421 cggaggtgta tcgagtacgc ggtatacccg gcgggccgag acaattgcac tcgcccaggt
     3481 gtttgccagc atcgccgtca tgatcagagg cgtcatcgtc gtcatcagat aattcgtaaa
     3541 cgcaactatc tgccctatcg agagatcgcc gcgtatcgac cgctgtcctc caacccagat
     3601 caccaccaca acagccgtgt tgacgagcag cgtcagcacg gggcccatgg tggacatgaa
     3661 ctccatcacg cggatggaac ggcgggtgag gtcctcgttg gcgtccccaa accgcgcccg
     3721 ttcatgatcc gcccggacaa atgccttgac cacctgaaca ccggccacgt tttcctggag
     3781 cacgttgttc aaccggtcca gcttctcttg cacagcgcga aacagtggtt ccatccgcgt
     3841 tgcaaagaag cctacggtgg cggaggtcaa aagcagcaac ggcaccatgg acagcgcgag
     3901 gccggggctt gtacggacca tgaggaacag gctcccgatc atgaggaggg gtgaccgcgt
     3961 tccgatccgc aacgaaatat ggacgaggcc tttgagggcg ttgacgtcgc tggtaagccg
     4021 aaccatcagc tgccccgttt tctgctcgtc cagattcccg taagaaaacg actggatgtg
     4081 gcggaaaatc gcttcgcgca ggtccctcgc gacgctttcg ctcacccgga cagagaacac
     4141 gttgtttccg accgcaaaga ccgcactgac aactgaaatc gcgagcatga taagcgacgt
     4201 aacaactatc acgcgctcat catgacggta aatcccgtgg tcgatgatcc gctgaatcag
     4261 acgggggatg gagaggtcca tggccacaag acaggtaagc aacaccaggg atgccagaga
     4321 ttgcttccag tagggccgga caaaggagct gaatcgtctg aggttaccca tagttcccga
     4381 ttgtggaata cggcggacgg cactctggtg tggaaggaac gccggttgct atcaaacaaa
     4441 tatcgccaca tcctctgccc ggacgaactc cgtcccatcg tttgtgcaac atctctttcc
     4501 acgcgtgcag tccaacgcac ttgacatccg tttcaaatac acttaatgtt aagtgtagta
     4561 atctgccggg agcgaaccag agcgctttca ctcgcaggag gcacaaacta tggccagtct
     4621 caccgcaatt cagaaagaca ttctgcagtt tcttgcactc tttcagcaca gggaaggtcg
     4681 gtcagcaacg ggcactgaag tacaacaaca tttcgggtat acgcaccaca gtactgcccg
     4741 tcagcacctg caggcgttgg agcgcaaggg cttcattcaa ctcgcgcgcg gcggccatgg
     4801 cgttccttac catattcgtc ttctt
//
LOCUS       DAOTNG010000245         4824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14506_length_4824_cov_5.209688, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000245 DAOTNG010000000
VERSION     DAOTNG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14506_length_4824_cov_5.209688"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..>550)
                     /locus_tag="P5179_11060"
     CDS             complement(354..>550)
                     /locus_tag="P5179_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95799.1"
                     /translation="AWELCALTWPPDVAGRQAFLTTFVTNLPKGRYWRELLEMNGREA
                     MREAIGWLPGEEDIPPSLRE"
     assembly_gap    551..560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<561..1374)
                     /locus_tag="P5179_11065"
     CDS             complement(<561..1374)
                     /locus_tag="P5179_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95800.1"
                     /translation="MKQLQLCLLGGLTMLLAGVADSGNVPVTDDYASPVLDWWGGFRR
                     YSGPRQFESAIQRVEENQKFVPTGELRARKSFRNYVVKIYGHGYGIYDDGGSVEITRN
                     GTRVFAGHSGRIEIACVDGGEGDTCTTTLIGRDVTGDGIPDLVLTGYSGGMHCCHSLD
                     FFELGSQFRHIQSVYSDDAHFSRFVNLDADPALEIEVDDASYAYGRVWTGPIFNPIIL
                     KFQNGQYRMAGNLMRKPPMTRRELETLADSARAYSYLEDGLRHDMLSLIYTGN"
     gene            complement(1743..2981)
                     /locus_tag="P5179_11070"
     CDS             complement(1743..2981)
                     /locus_tag="P5179_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95801.1"
                     /translation="MGISLGLVGLGSFGSAFAPLFKSHQAVDRIAFCDLNPASLAKFV
                     NDPFYRDKFDPKDTFASLDDILAADLDALAIITQPQLHAPQCVAAMEAGKHVYSAVPV
                     ISLPDFDEILEWCDRIIRTCEKTGMRYMLGETTVYRPKTMFCRRMADRGEFGTFVYTE
                     GEYIHDVDAQCNLRDVAASRAANPSAVPRGTVLTTRSGTEPKGSPMSYPTHSVSGPVH
                     VMRAHAVKASAIGFRNRTGDPYFARQEFSNVVAFYRMSNGATCRICEFREAPGYFGDD
                     AETFRIAGTAGTFSNGMWWRNGRTQAGRVPPPTCQTPTDEEMRDPLPSEVVNAFKRVE
                     YRGTPESELAHLDFTPTGHGGSHPYLVHEFVTAVAENRQPVINAWEAARYMAMGAMAH
                     KSALRDGEWLEVPDWGDAPG"
     gene            complement(3043..4392)
                     /locus_tag="P5179_11075"
     CDS             complement(3043..4392)
                     /locus_tag="P5179_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRS95802.1"
                     /translation="MPERKAVTQLARKKRVARRDVLDGEMQEAIRRFIPSEAFGLLGK
                     SCVTDVRPGDHVEKTMTILFSDIRDFTPLSERMSPAENFDFINAYFRRMESVFVRHNG
                     IIDKYMGDAVMALFPTSPDDAVHCALEMLQRLSGFNNALKRKRGQAPIRVGIGLNTGL
                     TMLGVVGGRSHLESTVISDAVNLAARIETLTKSYGTPLLISEHTYYGARNIGHHDTRF
                     VDRVKVKGKEQPQSVYEVFDADPPLLREAKRRTKGLFEEALARYHMKEVPAAMELLAQ
                     CLAEAPDDTAAQVYRARCERFMETGVHESSGEFDLMIEWNPAVAVGCEEIDEQHRALF
                     DFVKGLVQAIQKTRNLSEIRTAISFLDNYVAEHFTAEEKLMAEYDYPFLPMQVAEHQR
                     FVDRFGKFKEEIRKNFRTRRNFLLFHAQILLIDWVVNHTSKLDRHFGRYLNRRDAER"
     gene            complement(4593..>4824)
                     /locus_tag="P5179_11080"
     CDS             complement(4593..>4824)
                     /locus_tag="P5179_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95803.1"
                     /translation="YAEFGDGTTPLRWIDIESGQETMLVRMRTVPLVEGPMNSLRVDP
                     HPAWDGSFRRIVFNACPDGRRKIFVADLTCSV"
BASE COUNT          925 a         1530 c         1366 g          993 t
ORIGIN      
        1 gagagaccct tttccatgtg gaagcagtca gcgaagggag acaaaaagat acgggagagg
       61 ggccagatag gcaaagattg gggggaggat gcggacgcca ctcagtctgc aggaaggtag
      121 ggcggcattg gtttcgtgcg caaccgcgca tgcagggcgc cgcaacacca catcccacag
      181 gaggcccaac gagatcgcgg tggtctgggg acgaaccgcc ctaaccggtt catcatctga
      241 tgcccctgga gggacccgct tcgtcgggtc cacaccattt cccctctccg gcaggcttcc
      301 tcggggttgc gccagcccgg accacccgcg cgagttggac agtgcggtct tcactattct
      361 cgtagtgaag gagggatgtc ttcttctccc ggcaaccagc cgatcgcttc gcgcatagcc
      421 tccctgccgt tcatttccag gagctcgcgc cagtagcgcc ctttgggcag attggtaacg
      481 aaggtcgtta agaacgcctg tcgccccgcc acgtcgggtg gccatgtgag agcgcaaagc
      541 tcccaggctt nnnnnnnnnn cattgccggt gtatatcagg ctcaacatat cgtgcctcaa
      601 accgtcctcc aaataggagt acgcccttgc tgagtcggca agtgtttcca attccctccg
      661 ggtcatggga ggcttccgca tcaaattccc cgccatgcgg tattgcccgt tctggaactt
      721 caagatgatg ggattgaaga tgggcccggt ccatacacgc ccgtaggcgt aactggcgtc
      781 gtccacttca atctccagcg cggggtcggc atcgaggttc acgaaccggc tgaaatgcgc
      841 gtcatcggaa tagacagatt ggatgtgccg aaactgcgaa ccaagctcga aaaagtcaag
      901 cgagtggcag caatgcatgc ctcctgaata accggtgaga acgaggtccg gtattccatc
      961 ccccgtaacg tccctgccga tgagtgtagt tgtgcaggtg tctccttcgc ctccatcaac
     1021 acatgcgatc tcgatgcggc cgctatggcc ggcgaaaacc cgtgttccgt tgcgcgtgat
     1081 ctcaacagat cccccgtcgt cgtatatgcc gtacccatgt ccgtatatct tcaccacgta
     1141 gttgcggaag gacttccgtg ctctcagttc ccccgttgga acgaatttct gattctcttc
     1201 caccctctgt atcgcggatt cgaattgacg tggtcccgaa tacctccgga accctcccca
     1261 ccagtcgagg actggagagg cgtaatcatc ggtcacgggc acatttcccg aatccgcgac
     1321 gcccgccaga agcatagtca ggccccccaa caagcacaat tgaagctgct tcatggttct
     1381 cctctcccag tattcgcaaa tcgtcaagcc accttggacg caggtcgccc tacctggctc
     1441 atcacctggt gcctctggag gggcccgctc ttctttgcat cgagccgtgc cgtgcagggc
     1501 ggaggtcacg acgaagcgtg acccaccagc ccggcggacc ggagtgtttt tgcgaatgga
     1561 cgtgcggcgg cctgtctcgc tacaagggtt cagtcccctc gttcagagtc gcgggacacg
     1621 cctgatacac gaatatccga tcgcggtggc ttccggtcga accttgaaaa aggtccatcg
     1681 ttattgaaga ttcggctcca aacttgaata ccaggttctg cagccgcagc cgagttaccc
     1741 cctcacccgg gcgcgtcgcc ccagtcggga acctcgagcc actcgccatc ccgcagcgcg
     1801 gatttgtgcg ccatcgcgcc catggccatg tagcgtgcgg cctcccacgc gttgatgacg
     1861 ggttgccggt tctcggccac ggcggtgacg aactcgtgca caaggtacgg atggctgcca
     1921 ccgtggccgg tgggcgtgaa gtccagatgc gccagttcgc tctccggcgt gccgcgatac
     1981 tccacccgct tgaacgcgtt caccacttcg gagggcagcg ggtcccgcat ctcctcatcg
     2041 gtcggggtct ggcaggtggg cggaggcacg cgtcccgcct gcgttcgccc gttgcgccac
     2101 cacatgccgt tgctgaaggt gcctgccgtg ccggcgatgc ggaacgtttc ggcatcgtcc
     2161 ccgaaatatc ccggggcttc acgaaactcg cagatccggc acgtggcgcc attgctcatc
     2221 cggtagaatg cgacaacgtt ggagaactcc tgccgcgcga aatacgggtc acccgtgcgg
     2281 ttgcggaaac cgatcgcgct tgccttcacg gcgtgcgcgc gcatcacgtg caccggtccg
     2341 cttaccgaat gcgtggggta cgacatgggg cttcccttcg gttccgttcc gcttcgtgtc
     2401 gtgagcactg tgccccgcgg taccgcgctc gggtttgccg cccggcttgc cgccacgtcg
     2461 cgcaggttgc attgcgcgtc gacatcatgg atgtattcac cctccgtgta cacgaacgtt
     2521 ccgaactcgc cccggtcagc catgcggcgg cagaacatcg tcttcgggcg gtagaccgtg
     2581 gtttcgccca gcatgtaccg catacccgtt ttctcgcatg tgcggatgat ccggtcgcac
     2641 cactcgagaa tctcgtcgaa atcgggcaat gagatcaccg gaaccgcgct gtaaacgtgt
     2701 ttgcccgctt ccatcgcggc gacgcactgc ggcgcgtgga gctgcggctg cgtgatgatg
     2761 gccagcgcgt caaggtcggc agccaggatg tcgtccagcg atgcaaacgt atccttcggg
     2821 tcgaacttgt cacggtagaa cgggtcgttg acgaatttcg cgagggaggc cgggttcaga
     2881 tcgcagaacg caattctgtc caccgcctga tggctcttga aaagcggcgc gaacgcactg
     2941 ccaaacgaac caagacccac gagcccgaga ctgatcccca cgcgttcctc cttctgcgtt
     3001 ccgtgttgcc tgctgaatga ccctgcgaat tgcgcgcgag aactaccgtt cggcgtcccg
     3061 ccgattcaga tacctaccga aatgccggtc gagcttcgag gtgtggttca ccacccagtc
     3121 aatcagaagg atctgcgcat ggaaaaggag gaaattcctg cgcgtgcgga agttcttgcg
     3181 aatctcctcc ttgaatttcc cgaacctgtc cacaaagcgc tgatgttcgg caacctgcat
     3241 cggcaggaac gggtagtcat actcggccat cagcttttct tccgccgtga aatgctcggc
     3301 cacgtaattg tcgaggaacg agatcgcggt ccggatttcg gaaaggttgc gcgtcttctg
     3361 gattgcctgc accagcccct tcacaaaatc gaacagggcc cggtgctgct catctatttc
     3421 ctcgcagcca acggcaactg ccgggttcca ctcgatcatc aggtcaaatt cgccgctgct
     3481 ttcgtgaacc cccgtttcca tgaagcgttc gcaccgcgcg cggtagacct gtgcggcggt
     3541 atcgtcgggt gcttctgcca gacactgggc aagcagctcc atggcggcgg gaacctcttt
     3601 catgtgatac cgcgccaacg cctcctcaaa cagccccttg gttctccgtt tcgcctcccg
     3661 caggaggggc ggatcggcat caaatacctc atacacagac tgcggctgtt ccttcccttt
     3721 caccttcacg cggtcgacaa accgcgtgtc gtggtggccg atatttcgcg ctccgtagta
     3781 cgtgtgctcg ctgatcaaca acggtgtccc gtaacttttc gtgagcgtct cgatgcgcgc
     3841 ggcgaggttg acggcatcgc tgataacggt gctttcgaga tggctgcgtc cgccaaccac
     3901 tccgagcatc gtaagcccgg tgttgagccc gatgccgacc cgtatcggag cctgccctct
     3961 ctttctcttc agggcattgt tgaacccgct gagcctctgc agcatctcga gggcgcaatg
     4021 gaccgcgtca tccggactgg tgggaaacag cgccatgacg gcatcgccca tgtacttgtc
     4081 aatgatgccg ttgtgccgaa cgaacaccga ttccatacgc cggaaatatg cgttgatgaa
     4141 atcgaagttc tcggccggac tcattcgctc ggaaagcggg gtgaagtccc tgatatccga
     4201 gaagagaatg gtcatcgtct tttcaacgtg gtctccggga cggacatcgg taacgcagct
     4261 tttgcccagc aacccgaagg cctcggaggg aataaaacgg cggatagctt cctgcatctc
     4321 gccgtccaga acatcccgcc gtgcgacgcg ttttttgcgc gccagttgtg tgaccgcttt
     4381 ccgttcaggc atgctcgatc cctccctcgt tgtctgcgcc acagctcacc actctgcaac
     4441 cacccgccag atggcgcaaa aaagagtccc ggaacaccgg tcccggcacg ttgccggcaa
     4501 gcaacgctgc tgcggcgcat tcatcgcgca aaatatacgc gaattccaga actggacaag
     4561 cggaacagac tgacgtggtc agaattcggg cgttacacgc tgcatgtcag gtctgccacg
     4621 aatatcttcc gccgcccatc cggacacgcg ttgaacacaa tccggcgaaa ggacccgtcc
     4681 catgccgggt gcgggtccac ccgcagggaa ttcatcggcc cttcaaccag cggcaccgtg
     4741 cgcatgcgca caagcatcgt ctcctgcccg ctttctatgt caatccaccg cagcggcgtg
     4801 gtgccgtccc cgaattccgc gtac
//
LOCUS       DAOTNG010000246         4822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14514_length_4822_cov_5.704007, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000246 DAOTNG010000000
VERSION     DAOTNG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14514_length_4822_cov_5.704007"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2496)
                     /locus_tag="P5179_11085"
     CDS             complement(<1..2496)
                     /locus_tag="P5179_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS95804.1"
                     /translation="MCDAWQRHSSDAQSTDALGGGNFIPSDEHQSGSKQLPDSAVFFA
                     LLESYPTGVLVVNQSGVIVAANAKAARFLKGVHLDVTGTSFLDFLSAESWQGFERTAK
                     TLVDAGQGQARLPVKGANGVIHVFEAVSKYPTTSDENDTVLILRDTTDYDMTLAANAE
                     DRLRILSRAVEQSQTVVLITDAEGTIEYTNEAFTAISGYSAEEARGKNPRILKSGHTT
                     EAEYRHLWDTIRSGETWYGTFYNRRKNGTFYWEAAKISPVRNNEGVITHFIALKEDIT
                     ARVEAEEELARRARYRMAIVEADRILLERESGEDLTPVLRVLGEASEAARVREFHNQR
                     DESGGLFASPVAEWVAPGVQLQIGNPALKYLPYEGTFSRWEKTLRSGRPIFGSVKDFP
                     PEEQRLLEAQHIAAVAVFPITKGTEWIGFIAFERCDEPPQPWTESEIALLGAAAASIS
                     LGVSRQAAQKALVESEERYRSVVNSVKEVIFQTDAEGNWTFLNPAWEGLTGFTVDQSI
                     GTSYLQYTHPEDKTENLELLASLSSGELESIRHETRYLTKNGEERWCEALVWVLRNDS
                     GAVRGAAGVIEDITEQKLAIEELRRAKEAAEAANRAKTEFLANISHEIRTPMNGILGM
                     TELALETQLSREQREYMQTVKTSADALLVLLNDMLDLSKIEAGQLELERIEFDFRATV
                     VTTVNTLLTRAEAKNITLDVRVDPNVPATVVGDPARLRQILLNLVGNAVKFTEVGGVS
                     VEVVAENGGKPGRTSIRALVRDTGIGIPVERQSAIFESFTQADATISRRFGGTGLGLA
                     ITKRLVNAMGGTIKVTSRPGQGSTFEFVIPFEKG"
     gene            2683..3063
                     /locus_tag="P5179_11090"
     CDS             2683..3063
                     /locus_tag="P5179_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS95805.1"
                     /translation="MYSMEHVALAAKDTRTLANWYERVLGFKIVYESEKEPKAFFVQD
                     SKGMAIEIVPPLEDGNTVDANANHLAIWVDDFDRACADLRAKGVVLEPELTNPFFGKT
                     RIAFFNDCEGHRLQIIWRVRRIGE"
     gene            3105..3326
                     /locus_tag="P5179_11095"
     CDS             3105..3326
                     /locus_tag="P5179_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRS95806.1"
                     /translation="MSDVGKWIVVAGVILVIVGLAFMVSPKFPWLGRLPGDLVWKKGP
                     VTVYVPLATMLLISIVATALVNIIARWLR"
     gene            3651..>4822
                     /locus_tag="P5179_11100"
     CDS             3651..>4822
                     /locus_tag="P5179_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRS95807.1"
                     /translation="MSESEIAPSFEEAKRLVNAGAMLPVHRRILADTETPVSAYMKIR
                     AEDRYSFLLESVEGAEKFARYSFLGVDPFLKFSASGTRSEAHDRRSNTSDVRNGNPIS
                     HFRELLHQHRGIPLEGLPRFSGGAVGYFAYDAVRWVEKIPDSGLDDIGLDDAVFLFYD
                     TVLVFDSVAHQIILVSNVRLTDDESELRARYDAAVRKIAHLEKLLAYPVNPRPVRGTG
                     ISELSANMTREQFERAVEVAKEFIRAGDIIQVVLAQRFRVKIASGAFDIYRRLRIVNP
                     SPYMFFLALDGTEILGASPELLCRVESGVVHVRPIAGTRRRGATEDEDRHLEDELRAD
                     PKERSEHIMLVDLGRNDVGKVAIPGTVKVSEFMVVERYSHVMHLVSHINGRLREDQ"
BASE COUNT          975 a         1378 c         1356 g         1113 t
ORIGIN      
        1 ccctttctca aaggggatga cgaactcgaa cgtgctcccc tgccccggtc tgctcgtgac
       61 tttgatggtg ccacccatcg cattcacaag ccgcttcgtg attgccagcc ccagcccggt
      121 acccccgaat ctgcgactaa tggtggcgtc cgcctgtgtg aagctttcga aaatcgctga
      181 ttgccgttca acagggattc cgatacctgt atcacgcacc agcgctcgaa tgctggttct
      241 acccggtttt ccgccgttct ccgcgacgac ttcaacggaa acgccaccga cttcggtgaa
      301 tttgaccgcg ttgcctacca gattcagcag aatctgacgt agtcgagcag gatctccgac
      361 cactgtagcg gggacgttcg ggtcaacccg gacgtccagg gttatgtttt ttgcctccgc
      421 gcgagtgagg agtgtgttca ccgttgttac caccgtagcg cgaaagtcaa actcgatgcg
      481 ctcaagttca agctgccccg cttcgatctt cgaaaggtcc agcatatcgt tcagaagcac
      541 gagaagggca tccgcggacg ttttgaccgt ttgcatgtat tctcgttgtt cgcgcgagag
      601 ttgagtttcc agcgccagtt ctgtcatacc gagaatcccg ttcatgggag tgcgaatctc
      661 gtggctgatg ttggcgagga actcggtttt cgcccggttc gcagcctccg cggcttcctt
      721 cgcccggcgg agttcctcaa tcgcaagctt ctgttcggtg atatcctcga tcactccggc
      781 agcgcctcga acggcgccgg agtcattccg aagcacccac acgagcgctt cacaccatcg
      841 ctcttcgcca ttttttgtca gataacgcgt ctcgtgtctg atggactcca gttctccact
      901 cgagagagag gcgagcagtt ccagattttc ggttttgtcc tcgggatgcg tgtactggag
      961 ataactcgtc ccaatgcttt gatcgactgt gaacccggta agcccctccc atgcgggatt
     1021 tagaaacgtc cagtttccct ccgcgtctgt ctggaaaatc acttctttca cgctgtttac
     1081 cacggaacgg taccgttctt cgctttcgac gagagctttc tgtgccgcct gccgcgaaac
     1141 ccccagtgaa atggacgccg ccgccgcccc cagcagcgct atttcagatt cggtccatgg
     1201 ctgcggcggt tcgtcgcatc gctcgaacgc gatgaagccg atccattcag ttccctttgt
     1261 gatcgggaat acggcaacgg ctgcgatatg ctgagcctcc agcaggcgct gttcttccgg
     1321 cgggaaatcc ttcactgatc cgaaaatcgg cctcccactc ctcaacgtct tttcccagcg
     1381 cgaaaacgtg ccttcgtagg gcaggtactt cagcgcggga ttcccgattt gcaactgcac
     1441 acccggcgcc acccattctg ccaccggact tgcgaaaagc ccgccggatt cgtcgcgttg
     1501 attgtggaac tcgcgcactc gtgcggcctc ggacgcctcg cccagcactc tgagaactgg
     1561 tgtcaggtcc tccccggatt ccctctccag aaggatgcga tccgcttcga cgatggccat
     1621 gcgatagcgc gctcttcggg caagctcctc ttctgcttcc acgcgggcag tgatgtcttc
     1681 cttgagcgca atgaaatgcg tgattacacc ttcgttgttt cggaccggtg atattttggc
     1741 ggcttcccag tagaacgttc cgtttttcct tcggttgtag aacgtgccgt accacgtctc
     1801 tcccgagcgg atagtgtccc agaggtggcg gtattctgct tccgttgtgt gcccggattt
     1861 gagtatccgg gggttcttgc ctcttgcttc ctccgccgaa tagccggaaa ttgccgtgaa
     1921 tgcttcgttt gtgtactcga ttgttccttc cgcgtccgta atcaacacca ccgtctggct
     1981 ctgttcgacg gcacgggaaa gtatccgcaa ccgatcctca gcgttcgctg ccaaagtcat
     2041 gtcgtagtca gtggtgtccc gcagaatcaa aacggtgtca ttttcgtccg acgtcgtcgg
     2101 gtatttcgag accgcctcga acacatggat gactccgttg gcgcctttca cgggcaaccg
     2161 ggcctggccc tgccccgcgt caacgagtgt tttcgccgtg cgttcaaacc cctgccatga
     2221 ttccgcgctc agaaaatcca gaaacgatgt tccggtgacg tccagatgca cgcctttgag
     2281 aaaccgcgcg gcctttgcgt ttgccgcaac gattacgccc gattggttca ccaccaaaac
     2341 acccgtggga taggactcga ggagcgcgaa aaacaccgca gaatccggga gttgctttga
     2401 tccactctgg tgctcgtcgg acggaatgaa gttgccaccg cccaacgcgt ccgttgattg
     2461 ggcatcgctc gaatgccttt gccaggcgtc gcacattgat ctgctcccgg aaagacttca
     2521 ccacgcagag gtacgcataa gcgctacttc cgctaagata atacgtgatt catcccgtca
     2581 ccgcgaaggc aaggtgaaac agggttttgc ccgaaaatcg ggtcgctggg tatattcgga
     2641 ggtggagtcg tctggacatc aatctcggaa agggaaacaa gcatgtattc gatggaacac
     2701 gttgctcttg cggcgaaaga cacgcgcacg ctggcgaatt ggtatgaacg cgttctggga
     2761 ttcaaaatcg tgtatgagag cgaaaaggag ccgaaagcgt ttttcgtaca ggattccaaa
     2821 ggcatggcga tcgaaatcgt gccacccctt gaagatggga acacggtaga cgccaacgca
     2881 aaccatcttg ccatctgggt ggatgatttt gacagggctt gcgcggatct gagagcaaaa
     2941 ggggtggttc tggaacctga attgaccaat ccgttcttcg ggaagacacg cattgcattt
     3001 ttcaacgatt gcgaagggca ccgcctgcag atcatctggc gggtaaggcg aattggagag
     3061 tgaagccgga cctgaactga cttcacgacg gaggttgaca tcgggtgagt gacgtgggca
     3121 aatggatagt ggtcgcagga gtgattctgg taatcgtcgg gctcgccttc atggtctcac
     3181 cgaaatttcc ctggttgggg agattgcccg gcgatctcgt gtggaaaaaa gggcccgtca
     3241 cggtgtacgt acctcttgcg accatgctgc tcataagcat tgtggcaacg gcgcttgtga
     3301 acatcatcgc acggtggttg cgctaaggta ccaactgcgg tgggaatatg gtggtgctgc
     3361 ggtgtacgga gcaccgggaa atcactcgcc gccaaagagg gtaacggttt tttttgcgcc
     3421 gcccttgacg gaagagtatc ggagccctat tatcaatttc cacctgcttc agaatgaagg
     3481 tatcgatgca cgtctcgttc cccatggatg cacagcgctg gtattggcgc cgctttcata
     3541 aggagacgtg tgcggtaacc tgacctctgt acagggcaat ggtttcgggc tgtgagcgca
     3601 cacgtcgctc acagcctttt ttgttgtacg aatttcgaag cgtgtggccc atgtcggaat
     3661 cagaaatcgc gccgagtttt gaggaagcga agagactggt gaatgccggc gcaatgctcc
     3721 cggtgcaccg ccgaatactc gcagacactg agacgccggt atccgcgtac atgaagatcc
     3781 gcgcggagga taggtactct tttttgcttg aaagtgtcga aggcgcggaa aaattcgcac
     3841 gctacagctt cctcggcgtg gatccctttc tcaagttctc tgcatccggc acccgatcgg
     3901 aagcacatga ccgacgttcc aataccagcg acgtaaggaa cggtaacccg atctcccatt
     3961 tccgggagtt gctccaccag caccgcggga ttccgcttga gggtctgccg cgcttttcag
     4021 gaggcgcggt ggggtatttc gcgtacgacg ccgtgagatg ggttgaaaag attccggatt
     4081 ccgggctgga cgacatcggg ctcgatgatg cggtctttct tttctacgac accgttctgg
     4141 tgttcgactc cgtcgcccac cagatcatcc tggtatcgaa cgtgcggttg acggatgacg
     4201 agtcggaatt gcgcgcgcgt tatgacgccg cggtccgaaa aatcgctcac ctcgagaagc
     4261 tccttgcata cccggtgaac ccgcggcccg ttcggggaac cgggatttca gaactatccg
     4321 ccaacatgac tcgcgagcag ttcgaacgcg cggtggaagt cgcgaaggaa ttcatacgtg
     4381 ccggcgacat cattcaggtt gttctggctc aacgcttccg cgtgaagatc gcttctggag
     4441 catttgacat ctaccgccgg ctccggattg tcaacccctc tccgtacatg tttttcctcg
     4501 cgctggacgg caccgaaatc ctcggcgcct cccctgagtt gctgtgtcgc gtcgaaagcg
     4561 gcgtcgttca tgttcgaccg attgcgggca cgcggcgacg cggtgccacc gaggatgaag
     4621 atagacatct cgaagacgaa ctccgcgccg acccgaagga acgatccgag cacatcatgc
     4681 tcgtggacct cgggagaaac gacgtgggaa aggtggcaat cccagggacg gtgaaggtgt
     4741 cggaattcat ggtggtggaa cggtacagcc acgtgatgca cctcgtctcc cacatcaatg
     4801 ggcgccttcg ggaggaccag ga
//
LOCUS       DAOTNG010000247         4807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14567_length_4807_cov_4.436027, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000247 DAOTNG010000000
VERSION     DAOTNG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14567_length_4807_cov_4.436027"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="P5179_11105"
     CDS             complement(<1..577)
                     /locus_tag="P5179_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRS95808.1"
                     /translation="MNGESAVPMFHKIRRIHMVGIGGSGMSGIAEVLHSLGFAVTGSD
                     RLLSPVTARLESIGIPVAEGHDAANVRGAQVVVYSSAVKADNPELIAAQAGKIPVIER
                     SEMLGELTRMRFTIGIAGTHGKTTTTSMVGQLLTHAGMAPTIIVGGITKSLGSGGILG
                     KGGYFVVEADEYARTFLRMFPTIGVITTLEAD"
     gene            complement(574..1803)
                     /locus_tag="P5179_11110"
     CDS             complement(574..1803)
                     /locus_tag="P5179_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRS95809.1"
                     /translation="MEAPLIDANSLNWVHERLGDTGASRKQVVREAAGSGKAEGAEQA
                     LASSSQPLDVILLAATIMIVGFGATMVYSSSSVIAAEKFGGPAHFMVRQVTRAGLGLL
                     CLIAALFVPVDRVRKYFPYFAFASFSLLLLPALFSVAYKGARSWVNLLGLTLQPSEIV
                     KFALILYLADRLTKHQERLESLKDGVLPLIGLICIPLGIIVLEPDVGTAVAMASVFGV
                     MMFVANIRMRHILPLMGAVALAVYVLIIIEPYRLKRLEAGYQIQQGFVAMGSGGLLGR
                     GLGRSIQKYFFLPEPYTDSILPIIGEEFGFIGTVAVVLLFCLFGWRGLRIAYQQPTLF
                     RFLLATGLTANILMYALLNMAVMTALMPTTGLPLPFISYGGSSLVLNLAGTGLLLNLS
                     RQRRLREGEEEVKEDTE"
     gene            complement(1821..3200)
                     /gene="murF"
                     /locus_tag="P5179_11115"
     CDS             complement(1821..3200)
                     /gene="murF"
                     /locus_tag="P5179_11115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRS95810.1"
                     /translation="MDAYSPEQIAEITGGTVLNGSQGPVTGVSIDSRRVNKGDLFVAV
                     TTQTNDGHRFVGDAFAKGARAALVDRRKVEAHLREWDIYTLIAVDDPVAALQKLARAH
                     RRKFAIPVVAVTGSNGKTTTKEFIASALAGLGPVLRTEGTLNNHLGVPLTLLEITQEH
                     RAAVVEMGMNHAGEIHLLGTIAEATVGVITNTGQAHLESFPSFDALVDAKWELVGTLT
                     GDRMLVLNRDDVGLVARARRWDGTIRWFGIESDCAFKASALQQGQDGCWSFVVNGSEV
                     QLRIPGRHMVYNALAAIAAADALGIPAGEAAVGISRTEPADRRMRSLWIRGVLVLDDA
                     YNANPTSVRAALQTLMTLEPGKSGRRIAVLGGMRELGEQSLALHREVGSYVAGLGVNL
                     ITVGELGNIIAQGARSAGASETPGFETQEACAEWLRNNLREGDVVLVKGSRSEHMELV
                     IDHLQKALS"
     gene            complement(3219..4724)
                     /locus_tag="P5179_11120"
     CDS             complement(3219..4724)
                     /locus_tag="P5179_11120"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRS95811.1"
                     /translation="MTGAPWTAKAIADTVPGSLFFSGSAGDRPLTCIVTDSRTAREGA
                     LFCALPGTKVDGWRFAAHACERGASAVLAPVRIEGLAVPQIVVPDVRRALAVVASAWY
                     GNADRNTALVGVTGTNGKTTTVRLVGSVGAAGGFVPSTIGTLGATLRGEDFPFDTAWN
                     TTPEAHELRRLLKALNDAGSTLTAMEVSSHGLALDRVWGLSFRVAVFTNLTTDHLDFH
                     GSLDEYFRAKARLFEDLAPSSTAVINIDDRHGQKLVGRTAAKVLTYGFSPSADIHPLE
                     VADLPWIKGRVKLPQGTITLEMPLAGRFNVANAMAAIGTGLALGLAPEEIARGLKDAT
                     PAPGRFEPVQAGQDFAVIVDYAHTPDALENVLMAARSMTGNMGRVICVVGCGGDRDPF
                     KRPVMGRIASEIADVCVFTSDNPRSEDPEAILDAISEGVPKGRTFERIESRKAAIRHA
                     ISVARRGDIVVIAGKGHEPYQEIQGVRYPFDDRLEARAALEELAGKTIKKP"
BASE COUNT          973 a         1488 c         1329 g         1017 t
ORIGIN      
        1 gatccgcctc gagagtggtg atcacgccga ttgtggggaa catccgcaaa aacgtccgcg
       61 cgtactcgtc cgcttccacg acaaaatagc cgcctttgcc cagaattccg ccagatccca
      121 gactcttcgt gatcccgccc acgatgatcg taggcgccat tcctgcgtgc gtcagaagct
      181 gaccgaccat tgaagtcgtg gtggttttcc cgtgagttcc cgcgattccg atggtgaacc
      241 tcatccgcgt caactcgccc agcatttccg accgctcgat gaccggaatt ttacctgcct
      301 gagcggcgat cagctcgggg ttgtccgcct ttacggcaga ggaatagacg acgacctggg
      361 cgccccggac attggcggcg tcatgccctt ccgccacagg gataccaatt gattcaaggc
      421 gtgccgtcac gggactcaaa agcctgtcag aacccgtcac cgcgaacccg agagagtgca
      481 atacctcagc gattccactc attccgctcc cgccgattcc gaccatgtgg atccggcgga
      541 ttttgtggaa catcggaacc gcgctctcac cgttcattcc gtgtcctctt tcacttcttc
      601 ctcgccttct ctcaagcggc gctggcggga aaggttgaga agaagtccgg ttccggcaag
      661 gttgagcacg agggaggaac ctccgtaact gataaatggc agcggtaacc cggtagtcgg
      721 catcagtgcc gtcattacgg ccatgttcag caacgcgtac atcagaatgt tcgccgtgag
      781 gcccgttgca agcaggaatc gaaatagtgt cggttgctgg tacgcgattc gaagtccgcg
      841 ccagccgaag aggcaaaaga gcagaacaac cgcaaccgtg cctatgaacc caaattcctc
      901 accgattatc gggaggatcg aatccgtata cggttccggc aggaagaagt atttctgaat
      961 gcttctcccg aggcccctgc cgagcaaacc gccggaaccc atggcgacga agccttgctg
     1021 gatctgatat cccgcttcga gccgcttcag cctgtaaggc tcgataatta tcaagacgta
     1081 cacggcaagc gcgactgcac ccatcaaagg cagaatgtgt cgcatacgta tgttggcgac
     1141 gaacatcatc actccgaaca cggaggccat tgccacggct gttccgacat ctggttcgag
     1201 cacaattatg cccagcggta tgcagatcag tccgataagc ggtaacacgc cgtccttgag
     1261 gctttccagt ctttcctggt gtttcgtcaa acggtcagca aggtagagaa tcagcgcgaa
     1321 tttgacgatt tccgacggtt gcagggtcaa tcccagaagg tttacccagc ttcgcgcgcc
     1381 cttgtatgca acactgaaca gggccggcaa caaaaggagc gaaaaggacg caaatgcaaa
     1441 atacgggaaa tacttccgca cgcgatccac cgggacaaac agggcagcga taaggcacaa
     1501 aagtcctaat cccgcgcgcg tgacctgccg gaccataaag tgcgccggcc caccgaactt
     1561 ttccgcggcg atgacgctgc ttgagctgta caccatcgtt gcgccgaaac cgacgatcat
     1621 gattgtggcg gccagaagga tgacgtcgag cggctgagaa gaactggcca gggcttgttc
     1681 cgccccttcg gctttgcccg atccagccgc ttctcggacg acctgtttgc gagaggcacc
     1741 cgtgtcaccg aggcgttcgt gcacccagtt caaggaattc gcgtcgatga gtggtgcttc
     1801 cacagacgtt ctctccacgg tcaagagagt gctttctgga gatggtctat caccaactcc
     1861 atgtgctcgc tgcgcgaacc ttttacgaga acgacatcac cttctctgag gttattcctg
     1921 agccactcgg cgcacgcttc ctgagtctcg aatccgggtg tttctgaagc gcccgcggat
     1981 cgcgctccct gtgcaatgat gttgcccagt tcgccgacag tgatgaggtt cacaccgagc
     2041 ccggcgacgt acgagccgac ttcgcggtga agagcgaggc tctgttcacc cagttcacgc
     2101 ataccgccca ggacagctat tcttcggccg ctctttcccg gctcaagcgt cataagggtc
     2161 tgcaaagcgg cgcggacaga ggtgggattg gcattgtacg cgtcatccaa cacgagaacg
     2221 ccacgaatcc agagcgatct cattctcctg tccgccggtt cggtgcgaga tatgccgacg
     2281 gctgcctcac ctgccggaat gccgagggca tcggcggcgg cgatcgcagc gagcgcgttg
     2341 tacaccatat gccggccggg aattcgcaac tggacttccg agccgttgac gacaaagctc
     2401 cagcacccat cctggccctg ctgcaaggcg gacgctttga acgcgcaatc gctttcgatt
     2461 ccaaaccacc gaatcgtccc gtcccatctt ctcgcccgag ctacgagacc cacgtcgtcg
     2521 cgattcagta ccagcatgcg gtcacccgta agcgtaccga ccagttccca tttcgcgtcc
     2581 acgagagcat caaatgaggg gaagctctcc aggtgggcct gtccggtgtt cgtgatgacg
     2641 ccgactgtcg cttctgcgat cgttccgagg agatggatct cgcccgcgtg attcatgccc
     2701 atttcgacca ctgcggcgcg gtgttcctgc gttatctcaa gaagcgtcag cggaacgcca
     2761 aggtgattgt taagcgtgcc ttcagttcgc agtacaggac cgagtcccgc aagcgctgat
     2821 gcgatgaact ccttggtggt cgtttttccg ttcgagccag tcactgcgac caccggtatg
     2881 gcgaatttcc ttctgtgggc acgtgccagt ttctgaagtg cggcaaccgg gtcatcgacg
     2941 gcgatgagcg tatagatatc ccattcgcgc agatgggctt ccacctttct ccgatctacc
     3001 agtgcagctc tcgccccttt cgcgaacgca tccccgacga aacgatgccc gtcgttcgtc
     3061 tgcgtcgtaa cagcgacgaa gagatctccc ttgttgactc ggcggctgtc aatcgagacg
     3121 ccggttacgg gtccctgact gccattgagc accgtgccgc cagtgatctc ggctatctgt
     3181 tccgggctgt acgcgtccac gggatatgtt tccttccatt acggcttttt gatggttttc
     3241 ccggccaatt cctcgagagc ggcgcgtgcc tccaatcggt cgtcgaaggg gtaccggacg
     3301 ccctggattt cctgatacgg ttcgtgccct ttgcctgcga taacgacaat gtctcctcgc
     3361 cgcgcaaccg aaatcgcgtg ccggatcgca gcttttctgg attctatgcg ttcgaaagtg
     3421 cgacctttcg gcacgccttc ggaaatcgcg tccaggatgg cctccgggtc ttcagaacga
     3481 gggttgtccg aggtgaatac gcagacgtcc gcgatctccg aagctattcg tcccataacc
     3541 ggtcgtttga acgggtcgcg atctccaccg cagccgacaa cgcagatcac gcgtcccatg
     3601 tttcccgtca tggaccgtgc tgccatcagg acgttttcca gcgcatcagg tgtgtgggcg
     3661 taatccacaa tgaccgcgaa gtcttgaccc gcctgaactg gctcgaatcg acccggcgcg
     3721 ggcgtagcgt ctttcaaccc cctcgctatt tcttccggcg cgagcccgag cgccagcccc
     3781 gttccgattg cagccattgc gttggcgacg ttgaaccttc ccgcaagcgg catttccagc
     3841 gtgatggttc cctgcggcaa cttcactctg ccttttatcc agggcaaatc cgccacttcc
     3901 aagggatgga tatcggcaga aggcgagaat ccgtacgtaa gaactttcgc agccgtgcgt
     3961 ccaacgagct tttgaccatg tcggtcgtcg atgttgatta ctgcggtact cgacggtgcc
     4021 agatcctcaa aaagacgcgc ctttgcgcgg aagtattcat caagtgatcc gtggaaatcc
     4081 aggtggtccg tagtcaggtt cgtgaacacc gcgactctga agctcagacc ccagaccctg
     4141 tcgagagcca ggccgtgtga cgatacctcc atcgccgtaa gcgttgaacc ggcatcgttc
     4201 agtgctttca gaagtcggcg cagttcgtgc gcctctggtg tggtgttcca tgccgtgtcg
     4261 aacgggaagt cctcaccgcg gagagttgca ccgagcgttc ctatggtgga aggaacaaag
     4321 ccaccggcag cgccgaccga gccaacaagg cgcacagttg tggtttttcc attagtgcct
     4381 gtgacgccta ccagcgctgt gttcctgtcg gcgttcccgt accaggcact ggccacgact
     4441 gcaagcgctc tccggacatc gggaaccacg atctgaggta ccgcaagccc ctcgattcgg
     4501 actggtgcaa gtactgcgga agctccccgc tcacacgcgt gcgcggcgaa cctccaccca
     4561 tcgaccttcg tcccgggtaa tgcacagaaa agggctcctt cgcgagccgt gcgcgagtca
     4621 gtaacgatgc acgtcagcgg ccggtcacct gcactgccgc tgaaaaaaag gctgcccgga
     4681 acggtgtccg caatggcctt tgcagtccac ggtgcacccg tcactggctt cgtccccgtc
     4741 ggattgccgg tgcgcttggt gacgttggct ttgatgaacg ccgccgcttc ttcttccgcc
     4801 gagccac
//
LOCUS       DAOTNG010000248         4773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14692_length_4773_cov_5.699237, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000248 DAOTNG010000000
VERSION     DAOTNG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14692_length_4773_cov_5.699237"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="P5179_11125"
     CDS             complement(<1..1875)
                     /locus_tag="P5179_11125"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRS95812.1"
                     /translation="MHDEKKDFRVYVDVVYRKKWLILFIFLIVFLTTLFFVRRQAPSY
                     TSSSSIYIESGNPTAMAFSLSSDAQGKRPIEFFMGILESRSFRESVRDAVRDHAISRG
                     MPPIQATDLSAKAIESLQVKRSKNEGFYTLTAESESPDIAYATDSLTVGLFIERCILY
                     ARQQTSGMTAFVAMQFDSARARLERAEDNLQAFRKQHNLIQVGGDPATDPSLPIDYVR
                     LVEGYYDARQERQTAQAALEAAEEASRLLRINLDTLATDKLRRALPALDLAEAEERTR
                     KARTDLQLKELQEQSFLSQLQAYEASHPGLSDVSIGYLRLVRERDIYVRLSDLLLERR
                     EELRVKSASETGGVRVVDAPSEATPKPSRAPLILVFSAIIGLSLGVGAAFLLEMMDTS
                     VRTVGDITEALEVPTIGTIPSIGVAPGGKRRRESERRRVLISDGNPKDPVAEAYRTLR
                     TSLLYSSGDQPIRSLVISSAGQAEGKSITVANLAIVCAQMGQRTVILDADLRRPVQHI
                     LFELERDEGLTEALVHRHELAEVAKPTNIENLDVVTCGVIPPNPATLLGSAAMGERLQ
                     ELVQHYDIVLIDTPPVIVVTDAVLVARQTDGVLLVVRCNSTPREAARHTADTLRTANI
                     P"
     gene            complement(1969..2766)
                     /locus_tag="P5179_11130"
     CDS             complement(1969..2766)
                     /locus_tag="P5179_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95813.1"
                     /translation="MKCHFAGTIGFLGLCVFATLLVRGESRAETGLTGSTALLYVPVA
                     DVVPNERFSFGGLIADYQAFPSKQFSYFRANKPSAYFSGYATIGYIPRLEITVRGSGM
                     PSAMGPAGTGPFYTDGMISMQALLYRGRGRVPSIGAGFQDMYGFMIFNALYGVVTWSV
                     PGEHGNAVITGGWAVDWYDRNRGTADVNYIVPHVINGFFAGGAYPIRKWVDGIAEYDS
                     RSLNVGMRFHPVSWCTLDLAAVRWGLDRMARGQIAGFTAHLCFGGHL"
     gene            complement(2763..3695)
                     /locus_tag="P5179_11135"
     CDS             complement(2763..3695)
                     /locus_tag="P5179_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRS95814.1"
                     /translation="MVKANRPASTAFAALLACLCFIGLSVAAEPPGGERIIKAGDVLQ
                     ISVIGYAEFSKTVVVSPEGRIRYPVVSDRNIIGWTFSELENQITQQLATMLRAAPYVF
                     VDYAQTYTIRINILGQVRKPGVTEVPNGIDLQGALWIAGGPESSADLGDIQVQRRGLD
                     GTEEISVDLERFLFEGRLQDIVQMKDGDLVIVRGAPDADKVKVFGEVNKSGSYVVPFG
                     ATVLDMIYLAGGPTTAGTLSDVRWIRREGGRVVEQKLDFASLLRAGRTDEIPRVGRGD
                     VIIVQKRILTFSAVVATLSLAIQALTLYFLVQKL"
     gene            complement(3697..4629)
                     /locus_tag="P5179_11140"
     CDS             complement(3697..4629)
                     /locus_tag="P5179_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS95815.1"
                     /translation="MRVVVTGGAGFIGSHLCDALIRENHSVVCLDNLITGNLSNVEHL
                     FGHDRFRFIKHDVTEYIHIPGEVDFVFHFASPASPVDFERIPIQILKVGALGSHKTLG
                     LARAKGAGFLLASTSETYGDPLVHPQPETYWGNVNCVGIRGVYDEAKRFAEAMTMAYH
                     RYHHINTHIVRIFNTYGPRMRPDDGRAIPAFISQALKNEPITVFGEGRQTRSFQYIDD
                     LIRGILLLMESDEHLPVNIGNPNEYTILQLAEAIIELTGSKSEIVHRPLPPDDPKVRQ
                     PDIRKARERLGWEPVVPLREGLTKTIEWFKTHGA"
BASE COUNT         1007 a         1449 c         1348 g          969 t
ORIGIN      
        1 cgggatattg gccgtcctca gcgtgtctgc ggtgtgacgc gccgcttcgc gcggtgtgga
       61 gttgcaccgg accaccagaa gaacgccgtc cgtctgtcgt gccaccagga cggcgtcggt
      121 caccacgata acgggcggcg tgtcaataag gacgatatca taatgttgca ccagttcctg
      181 aagccgttcc cccatggcgg cggacccgag cagcgtcgcc gggttcgggg gaatgacgcc
      241 gcaggtgact acatcgagat tctcgatgtt cgttggcttc gctacttcgg caagctcatg
      301 ccggtgcacg agcgcctcgg tgagcccttc atcgcgctca agctcgaaaa gaatatgttg
      361 caccggccgg cggaggtccg cgtcgaggat gactgtcctt tgccccatct gcgcgcagac
      421 gatggcaaga ttggcgacgg taatcgactt tccttcggcc tgccccgcgc ttgatatgac
      481 aagggaccga atcggttggt ctcccgaaga ataaagaaga ctcgttcgca aggtccggta
      541 tgcttcggcg acgggatctt taggatttcc gtcggaaata agcacgcgcc ggcgttccga
      601 ttcacgcctt ctcttgcctc ccggcgcgac gccgatggac gggatcgttc cgatggtggg
      661 gacctccaga gcttccgtga tgtcgcctac cgttcgaaca ctcgtatcca tcatctccag
      721 aaggaacgcg gccccaacgc caagggaaag gccgatgatt gcggagaaga ccagtatcag
      781 aggagccctg cttggtttcg gtgttgcttc cgacggtgca tccacaacgc gcacgccgcc
      841 ggtttccgag gcggatttca cgcgcagttc ctcccgccgt tcgagcagca ggtcggacaa
      901 ccgcacatag atatcgcgtt cgcggacaag tctgaggtac cctatgctca cgtcggaaag
      961 cccgggatgg gaggcttcgt atgcctgcaa ctgagaaaga aagctctgtt cctggagttc
     1021 tttgagctgg aggtcggttc gtgccttgcg ggtgcgttcc tccgcttccg ccagatcgag
     1081 cgcgggaagg gctcgccgca gcttgtcagt cgccagtgtg tcgagattga tgcggagaag
     1141 acgggatgct tcttccgcag cctcgagagc ggcctgcgcc gtctggcgct cctgccgggc
     1201 gtcatagtac ccttcgacga ggcggacgta gtctataggc aggctcggat ctgttgcggg
     1261 atcgccgcca acctgaatga gattgtgctg tttgcgaaac gcctggaggt tatcctctgc
     1321 gcgctccaag cgagcacgcg cggagtcgaa ctgcattgct acgaacgccg tcatcccgct
     1381 tgtctgttgc cgggcgtaca ggatgcaccg ttcgataaaa agcccgaccg tgaggctatc
     1441 cgtcgcatat gcgatatcag gggactctga ctctgcagtg agtgtataaa acccctcgtt
     1501 tttgctgcgc ttcacctgaa gggactcaat cgcctttgcg ctcaggtccg tcgcctgaat
     1561 gggcggcatc cctcgcgaaa tcgcgtgatc gcggaccgca tcgcgaacag actcacgaaa
     1621 acttcgactt tcgaggatgc ccatgaaaaa ttctatcgga cgttttccct gtgcgtccga
     1681 acttaacgag aaagccatcg ctgtgggatt gccgctttcg atataaatgg aggaggagga
     1741 ggtgtaggac ggcgcttgcc tgcgcacgaa aaaaagcgtg gtcagaaaca cgatgagaaa
     1801 gataaagagg atcagccatt ttttccggta gacgacatcc acgtagacgc ggaagtcttt
     1861 cttctcatcg tgcattatcg cgttgctccc tgataccgga tccgactccc ccgttccccg
     1921 cgaagaagga accctgaaac acgacctgct accacgcaaa agactccgct acaggtgccc
     1981 gccaaagcat aaatgtgcgg taaaaccggc gatctgaccg cgcgccatcc tgtcaagccc
     2041 ccaccggacg gcagcaagat cgagagtaca ccacgaaacg gggtgaaatc gcatcccgac
     2101 gttcagagat cgcgagtcgt actctgcgat gccgtccacc cacttccgga tggggtacgc
     2161 accacccgca aagaaaccgt tgatgacgtg agggacaatg tagttgacat ccgcagtgcc
     2221 acggttcctg tcataccagt ctactgccca cccgccggta atcaccgcat tcccgtgttc
     2281 tccgggcaca gaccatgtca caacgccgta aagggcattg aagatcatga agccgtacat
     2341 gtcctggaaa ccggcaccga tcgacgggac acgtcccctg ccccggtaca acagggcctg
     2401 catgctgatc ataccgtccg tgtagaacgg cccggtaccc gcgggaccca ttgcggacgg
     2461 cattccgctt ccccggaccg tgatttccag ccgcgggata taaccgatcg ttgcgtaacc
     2521 actgaaatac gcgctcggtt tgttcgcccg aaagtacgag aactgcttcg acgggaatgc
     2581 ctggtaatcc gcgatcaaac cgccgaacga gaacctttca ttcggaacga cgtccgccac
     2641 cggaacatag agaagcgccg ttgagccggt caggcctgtt tcagcccggc tttcaccacg
     2701 aaccagcaag gtcgcaaaaa cgcacagacc aagaaaaccg atcgtgcccg cgaaatgaca
     2761 cttcaaagct tctgaaccag gaaatacagc gtcagagcct gaattgccag gctgagcgtc
     2821 gcaacaaccg ctgagaatgt cagtatcctt ttctgcacaa taatcacgtc gccacgcccg
     2881 acgcgcggaa tctcatccgt cctgccggcc cttagcagcg acgcaaagtc cagtttctgc
     2941 tcgaccacgc ggcccccttc tcgcctgatc caccgcacgt ccgacagggt gcccgccgtt
     3001 gttgggccac ctgcaaggta gatcatgtcg agaaccgtcg ccccgaaggg aaccacgtag
     3061 gaaccgctct tgttcacttc gccgaacacc ttcaccttgt cggcgtccgg ggcaccgcgg
     3121 acaatgacga gatccccgtc cttcatctgc acaatatcct gcagccggcc ctcaaacagg
     3181 aatctttcga gatccacgga gatttcttct gtaccgtcca ggccccgtct ctgcacctgg
     3241 atatcgccca gatccgcgct cgattcggga ccgcccgcaa tccacagggc cccctgcaga
     3301 tcaatgccgt tcgggacctc ggtaacacca ggttttcgca cctggccaag gatgtttatc
     3361 ctgatcgtgt acgtctgcgc gtagtccacg aatacgtacg gcgctgcgcg aagcatggtg
     3421 gcgagttgct gagtgatctg gttttcgagt tcagaaaacg tccagccaat gatgtttcgg
     3481 tccgaaacaa ccgggtagcg gattcttccc tcgggcgaca cgaccacagt cttgctgaat
     3541 tccgcgtacc cgatcacgga gatctggagc acatcgcccg ccttgatgat gcgctcaccg
     3601 ccgggcggtt ccgcagcaac cgacaacccg atgaaacaaa ggcaggccag aagcgccgca
     3661 aacgctgttg aagcaggccg gttcgccttc accatgtcat gccccatgcg ttttgaacca
     3721 ttcgatggtt ttggtcaacc cctcgcggag aggaacaacc ggctcccacc ccaggcgttc
     3781 gcgcgccttc cggatgtcgg gctggcgcac tttggggtca tccggcggaa gcgggcggtg
     3841 cacgatttca cttttgctgc cggtcaattc gatgatcgcc tccgcgagct gcaagatcgt
     3901 atactcgttc gggttcccga tattcaccgg caggtgctcg tcggattcca tcagcaggag
     3961 aataccacgg atcaggtcat caatgtactg aaagctccgt gtctgcctgc cttcgccaaa
     4021 cacggtgatc ggctcgttct tcagcgcctg tgaaatgaac gcgggtatcg cccttccatc
     4081 atccggtcgc atcctcggcc cgtaggtatt aaagatacgc acaatatgag tgttgatgtg
     4141 gtgataccgg tggtacgcca tggtcatcgc ttccgcgaag cgctttgcct cgtcgtacac
     4201 gccgcggatt cccacgcagt tcacgtttcc ccagtacgtc tcgggctgtg gatgcacaag
     4261 ggggtcaccg tacgtctccg acgttgaagc caggaggaac ccggcgcctt ttgcccgcgc
     4321 gagccccagc gttttgtgcg acccgagggc gccgaccttg aggatctgga tggggatgcg
     4381 ttcgaaatcc accgggctgg cagggctcgc aaaatggaat acgaaatcga cttcgccggg
     4441 gatatggatg tattcggtca cgtcgtgctt gatgaagcgg aagcgatcgt gcccgaacag
     4501 gtgttcgacg ttggagagat tacccgtaat gaggttatcc agacagacca cggagtggtt
     4561 ttcccggatc aacgcatcac agaggtgcga tccgatgaaa cccgctcccc cggtaacaac
     4621 aactctcatg cgctcctcct caccgtggcg gggcaactgc tgcatggctc acacacaaat
     4681 gctaccacgc ggccgggaga tggtcaagcg gagtacgtgc tgacggcctg acgtgccggt
     4741 ttttgcagga caggatttct aacttgacaa agt
//
LOCUS       DAOTNG010000249         4765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14723_length_4765_cov_4.525053, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000249 DAOTNG010000000
VERSION     DAOTNG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14723_length_4765_cov_4.525053"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="P5179_11145"
     CDS             <1..1782
                     /locus_tag="P5179_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS95816.1"
                     /translation="TEDPFMRRFLSLFCALVFATSAFATSVQSYNRPSWLHLPDNVPM
                     VERQWTNNSNKFTFVILGDRWSGGDGEENQATFDLAVREINMLRPDFVITIGDMIPGH
                     MRGREQWHQEWADYFRPASRLGVPLLFTVGNHDISNLEMYEWWKQDRGRTYYSFDYKG
                     AHFLVINTEEERVDGRGPVWEAQMQFIERDLAENVAAPHTFILMHKPMWIDNRYEADW
                     ARIERALGGRPRSVIAGHWHRVQTDWRNGNLYAVFGGTGCGGRNPVLELGAFQHYTHV
                     TVDGDSASFALIQPGKGIVSVDVPSRAFQDGLAGMVRTLSGGLQVARDGAATLSARVS
                     IANPLPDSTSVTVRAVLPPGTAWTKASGVDSFTVSLAPGQRAERVFTFAGTTTQLSPV
                     PVFVSNVDYRGVAAAPRRPVPVRLYPDSLMRAIREWMVIGAFELGPFNTRLLPDNPQE
                     ALPGLFARREPRNFFDARRPVVENGTEFRWRKVTADATGYMNFNAYLGTKDQALAYAM
                     TKVYSPVAQEVYAEFAADNYAQVVLNGEIVGQKFANPGSPGITPLRLKKGWNTLVLKL
                     VNNSGDWYLEFALADPRQNLRFSVDGR"
     gene            2119..>3509
                     /locus_tag="P5179_11150"
     CDS             2119..>3509
                     /locus_tag="P5179_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS95817.1"
                     /translation="MTPRAAIILSATLGFLTLAPSPYAATATIRVTVSPAHTDWPAAP
                     VRVPVDSLLRARGWTAPVSSSRVVVREAGRNGAVAPHRAAWGYQGVDEGTILFAKTGT
                     GTKTYEITVSDADPADAIRRLPVVGLGEPLTYGHTGVTGPMHGSFCAHAEPVDWDGDG
                     DTDLLVEEGQGGGATWTMHGMYYFENIGTPRAPLLAPARWVPVNRDPPCVVDWNNDGL
                     PDIVAGKFLHLNRGPRGAFNFAAPESLTATPGVEAMVDWDGDGLFDIFCTSSRNGEEP
                     SRSVWDKKTGFIPHTPEGVWRGFTPVSSIHVYRNTGRPGAPAFEAPPETLEANGVPIE
                     LQFGIHIDLDDVDNDGDLDLIAGNNFDIYFFENVGTRTAPRLARGFPLPLDQREILIW
                     PRFADMNGDGKRDIVMAIEYGDVRWFENLGRRDGVITFGPMRRFSLREPFIDDGCDGA
                     FDLADVNRDGKPD"
     assembly_gap    3510..3609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3610..3821
                     /locus_tag="P5179_11155"
     CDS             <3610..3821
                     /locus_tag="P5179_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95818.1"
                     /translation="RFAFRGEMVPDPGLRLAAHAACPQPVDWDGDGRLDMLIGGEDGL
                     IYAFHRAYLEQDLPKVRVEAVRVRR"
     gene            3824..4360
                     /locus_tag="P5179_11160"
     CDS             3824..4360
                     /locus_tag="P5179_11160"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRS95819.1"
                     /translation="MSIPRPAPPVLLIAAVTWSPEVAEAGIVARLEDAWGPISAMGPA
                     FRFDDYTDYYAEEMGAGLTKRIVAFAEPVRPEHLADIKLRSNELERFWHTAEGARRVN
                     IDPGYLCALTLVLASGKPSAYRIYLRDGIWAEITLRYERGSYAPLSTTYPDFRAAETI
                     AFLNSLRPRALEVGRETR"
     gene            4400..>4765
                     /locus_tag="P5179_11165"
     CDS             4400..>4765
                     /locus_tag="P5179_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95820.1"
                     /translation="MKYKPDWPDAKARLTALWHHELLDRPCIAVTAPSGRSFPHPPAP
                     SAPRDRWLDPGWVLQSMEAQLENTWWGGEAVPSCLMMAGWVLCLGGTPRFAQDTIWFD
                     TKDVDFSLPPPFRYEPDNPW"
BASE COUNT          822 a         1590 c         1433 g          820 t
ORIGIN      
        1 acggaggacc cattcatgcg acgtttcctt tcccttttct gtgcgctggt gttcgcaaca
       61 tcagcgttcg ccacgtcggt tcaatcgtac aaccgcccgt cgtggcttca cctgccggac
      121 aacgtcccca tggtggagcg ccagtggacg aacaacagca acaaattcac gttcgtcatc
      181 ctgggcgacc gatggagcgg cggcgacggc gaggagaatc aggccacctt cgatcttgcc
      241 gtgcgcgaaa tcaacatgct ccggcccgac ttcgtcatca ccatcggcga catgattccc
      301 gggcacatgc gcggccgcga gcaatggcat caggaatggg ccgattactt ccgtccggca
      361 agcaggctcg gcgtgccgct ccttttcacc gtgggtaacc acgatatctc caacctcgag
      421 atgtacgagt ggtggaaaca ggatcgtggc cgcacctact actccttcga ttacaagggt
      481 gcccatttcc tggtgatcaa cacggaagaa gagcgcgtag acggccgcgg acccgtgtgg
      541 gaagcccaga tgcaattcat cgagcgcgac ctcgccgaaa acgtcgccgc gccccacacg
      601 ttcatcttaa tgcacaagcc catgtggatc gacaaccgct acgaggccga ctgggcgcga
      661 atcgaacgcg cgcttggcgg ccgcccgcgt tccgtcatcg ccggccactg gcaccgcgtg
      721 cagaccgact ggcgcaacgg taacctctac gccgtgttcg gcggaacggg ttgcggcggg
      781 cgaaacccag tgcttgagct cggcgctttc cagcactaca cccacgtgac ggtggatggc
      841 gactccgctt ccttcgcgct catccagccg ggaaaaggca tcgtgtcggt tgacgtcccg
      901 tcgcgtgcat ttcaggacgg cctcgccggc atggtgcgta cgctgtccgg aggattgcag
      961 gttgcacgag acggcgcggc aacactgagc gccagggtct ccattgccaa cccgctcccg
     1021 gattccacct ccgtcaccgt gcgtgccgtg cttccccccg gcacggcgtg gacaaaagca
     1081 agcggcgtgg actccttcac cgtaagcctc gcgccggggc agcgcgcgga aagggtgttc
     1141 acctttgcag gcaccacgac gcagctctcc ccggttcccg tgtttgtctc aaacgtggat
     1201 taccgtggcg ttgcggcggc gcctcgcagg ccggttcccg tgaggctgta tcccgactcc
     1261 ctgatgcgcg ccattcgcga gtggatggtc atcggcgcgt tcgagctggg cccgttcaac
     1321 acccgcctcc tgccggataa cccgcaggaa gccctgcccg gtctcttcgc gcggcgcgaa
     1381 ccgcgcaact tcttcgatgc gcgccggccc gtggtagaaa acggcaccga attccgctgg
     1441 cggaaggtta ctgccgacgc aaccgggtac atgaacttca acgcatacct cggaacgaag
     1501 gatcaggccc tcgcatacgc catgacgaag gtctacagcc ccgtggcgca ggaggtgtac
     1561 gcggagttcg ccgccgacaa ttacgcgcag gtcgtcctga acggtgagat tgtcgggcag
     1621 aaattcgcca accccggttc gcccggtatc acgcccttgc gcttgaaaaa gggctggaac
     1681 acactcgtgc tcaagctggt caacaacagc ggcgactggt acctggaatt cgccctggcc
     1741 gatccccggc agaaccttcg tttctcggtg gacgggaggt agaaagggca ctgtggcacc
     1801 cggtggttga ggccgcggca cgcacactca accaccgcgc cgccacgccg ttccacaggt
     1861 cctgtacgac atttgcggct gcattgcgct gcctgactgc ccgatgccgg ggcgcctgtt
     1921 ctggattccc gcctgcgcgg gaatgacacg cctgctccac agttgactgc ggattctttt
     1981 ggtggggcag gcattcctgc ctgcccgggt cgggcttttg ccgccatgca cctgtgcgtt
     2041 gttcaccgcg ttgccgcatc gcctccatcc ccctaccttt cttccgacgc attgacccgt
     2101 gacaacgagg gcatacccat gacaccccgc gctgccatca ttctgtcggc cacactcgga
     2161 ttcctcacgc tcgcgccatc cccctacgcc gccaccgcga caatccgggt aactgtatct
     2221 cccgcccaca ccgactggcc ggccgcaccg gtgcgcgtgc cggtggatag cctgctgcgc
     2281 gcgcgcggct ggacggcgcc cgtgtcctcg agccgcgtcg ttgtgcgcga ggcgggccgg
     2341 aatggcgccg tggccccgca ccgcgccgcg tggggatacc agggcgtgga cgaaggcacc
     2401 atcctcttcg ccaaaaccgg cacggggacg aaaacgtacg agatcaccgt ctccgacgct
     2461 gaccccgcgg acgccatccg ccgcctcccc gtggtgggac tgggtgaacc gctgacctac
     2521 ggccacaccg gtgtcacggg acccatgcac ggcagcttct gtgctcacgc ggaacccgtc
     2581 gactgggacg gtgacggcga caccgatctc ctcgtcgaag agggtcaggg cggtggcgcc
     2641 acgtggacca tgcacggcat gtactacttc gagaacatcg gcaccccgcg ggcaccactt
     2701 ctcgctccgg caagatgggt gcccgtgaat cgcgacccac cgtgcgtggt tgactggaac
     2761 aacgacggcc tgcccgacat tgtcgccgga aagttcctcc acctgaaccg tggtccgcgc
     2821 ggcgcattca acttcgcggc gccggaatcc ctcaccgcga ctcccggtgt cgaagcgatg
     2881 gtggactggg atggtgacgg cctgttcgac attttctgca cctcttcccg caacggggag
     2941 gaaccgagtc ggtcggtctg ggacaagaaa accggattca ttccccacac acccgaagga
     3001 gtctggcgcg gcttcacgcc ggtcagttcg attcacgtgt accgcaacac cggccgcccc
     3061 ggcgcgcctg cgttcgaggc gcccccggag acgcttgagg ccaacggcgt gccgatagaa
     3121 ctccagttcg gcatccatat cgacctcgat gacgtggaca acgacggcga tctcgacctc
     3181 attgccggca acaacttcga catctacttc ttcgagaacg tgggcacccg caccgcgcca
     3241 aggcttgccc ggggcttccc cctccccctc gaccagcgcg aaatcctcat ctggccgcgc
     3301 tttgccgata tgaatggcga cggaaaacgt gatatcgtca tggcgatcga gtacggtgac
     3361 gtgcgctggt tcgaaaacct cggccgccgc gacggggtaa tcacattcgg ccccatgcgc
     3421 cggttttcgc ttcgggaacc gttcattgac gacgggtgcg acggggcgtt cgacctcgcc
     3481 gacgtgaacc gcgacggcaa gcccgacatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnna ccggttcgcg ttccgcggtg agatggtgcc cgaccccggt ctccgcctcg
     3661 ccgcccacgc cgcctgtccg caaccggtgg actgggatgg cgacggccgg ctggacatgc
     3721 tgatcggcgg cgaggacggg cttatctacg ccttccaccg cgcgtacctg gagcaggacc
     3781 tgccgaaggt gcgcgtggaa gccgtccggg tgaggagata ggcgtgagca tcccccgtcc
     3841 cgcgccgccg gtcctcctca tcgccgcggt cacgtggtcg cccgaagtgg cggaagccgg
     3901 aatcgtcgcc cggttggagg acgcctgggg gccgatcagc gcaatgggac cggcttttcg
     3961 cttcgacgac tataccgatt actacgccga ggaaatgggc gcgggtctca cgaaacgtat
     4021 cgtcgcgttt gcggagccag tccgcccgga gcaccttgcc gatatcaagc tgcgttcaaa
     4081 cgaactggag cgcttctggc atactgccga gggcgcacgg agggtgaaca tagaccccgg
     4141 atacctgtgc gctcttacgc tggtgctggc aagcggaaaa ccctcggcat accgcatcta
     4201 cctgcgggac ggaatctggg cggagatcac cctgcgctat gaacgcggca gttacgcgcc
     4261 gctcagcacc acgtaccccg atttccgggc ggcggagacc atcgcgttcc tgaacagcct
     4321 gcgtccccgt gcgctggagg ttgggcggga aacccggtag aatgtcggac ggactttcgc
     4381 cgcgcccccg gaaggacaga tgaaatacaa acccgactgg cccgacgcga aggcccgcct
     4441 gaccgcgctc tggcaccacg aactgctcga ccgcccgtgc atcgcggtga ctgcgcccag
     4501 cggcaggtcg tttcctcacc cgcctgcacc gtccgccccg cgggatagat ggctggaccc
     4561 cggctgggta ctccaatcca tggaggcgca actggagaac acctggtggg gtggtgaggc
     4621 cgtgccgtcc tgcctcatga tggccggctg ggttctctgc cttggcggta cgccccggtt
     4681 cgcgcaggat accatctggt tcgatacgaa ggacgtggat ttctcccttc cgccgccgtt
     4741 ccggtacgaa cccgacaacc cgtgg
//
LOCUS       DAOTNG010000250         4757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14756_length_4757_cov_4.289877, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000250 DAOTNG010000000
VERSION     DAOTNG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14756_length_4757_cov_4.289877"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1206
                     /locus_tag="P5179_11170"
     CDS             202..1206
                     /locus_tag="P5179_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRS95821.1"
                     /translation="MPTRIAILGAIVYDEIITHDGQRIESFGGIVYNIAALSSFIEDG
                     VVLPVSNVGFDRYDQVLALMAGMDHVDTSQLLRTDTPCTHAKLIYHGSNYREEIVRHM
                     MPPFSAPRIISAVNGDASTPAADGILINFVNGTELDLPTLQALRKQTKAPLYLDMHNI
                     MVRFDADGKKQYLDFVEWPEWVEQFDFVQMNEFECEKVLHTSPSSPKEFLHAAQTVLA
                     AGAAAVVITMGPAGVALAHRREGIEYGCIIPAVTVKNFVDATGCGDAFSTGTFWHYLR
                     TGNPLKAAMAGTLVGALNCEVRGVGKLERARNADQRIAEVSPQLADRVEHGWLGDRLN
                     "
     gene            1231..3186
                     /locus_tag="P5179_11175"
     CDS             1231..3186
                     /locus_tag="P5179_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95822.1"
                     /translation="MKRAEPEMAVLVRLDLSGIPFGPVLRPMEPVPEAERGLFSTWET
                     PLSPWGGFDVGAGLACVDDAGRPALECAEGERHERAIVARKVDLRDGTIVAEVKPLAA
                     NALPNDDSPVQTEALAGIVFRVQTSRAYYLFGIQGKRRVVLLRRNDDEFAVLAEQSIA
                     APQDYVTLEVRLDGDSIRCTSPELGVDMHVCDTVYHAGKVGFRSANSSRLRRFEVSQT
                     PGQRRRDESRHARFEREVAERGAGIPDATLVRTYRTADLGGSPLFRAFIEPGRFDILV
                     EGKQLRALTPEGGLIWEVPERIIRCVFSRDFGPAGRLIYGFAGKREERRAKDIAGGES
                     VLTVENEMVVIEGRTGKIVARAELPPNVPTQRFFDWSPRSASLANSDGTDIVLREWRD
                     DHGGGGIRLWAYDRGLNLLWEHVQTGAHYGHHWALDFFDVDGDDREEMLAGGCLYRGD
                     GTILWTHDRADEMQQIRGAGHYDAVVMGNLTGEAEDDPIAFLCAGSAGVYVVDALTGA
                     TRVVHRIGHAQGRSIGKLRPDLPGTEVLAVTRWGNYGILTLFSGRGERLWTIQPDYVG
                     QGATPVKWGKAGHRLIWTNTSRDAQLFFDGYGRLVKRLPELSRVWGDRMNRDVGRGGS
                     PVRIGTDPTDLLTLTVGGVVHVFGPAE"
     gene            3420..4310
                     /locus_tag="P5179_11180"
     CDS             3420..4310
                     /locus_tag="P5179_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS95823.1"
                     /translation="MLRKHSETIVNKVYPVPETPTYLPGSVIEIVNPDIDRGHIKHAV
                     FDFDGTVSVLREGWPDIMLPVMVDAIRGDHPATPEIVERCKKFIDETTGIQTFFQMEG
                     LVHMVEEFGLVPKDKILDAWGYKAIYNEALMKPVRERIAALERGEKTLEDVTMFGSRE
                     FLKALYDRGVRIYLASGTDQEDVRNEAHACQIDQWATGGIWGAIRNVDDYSKDKVMKD
                     IISQNNLHGAELMVVGDGPVEIRNAKDNGAIAVGVASDEVARRGLNAHKRQRLIKAGA
                     DIVIPDFLEWKKLLKYLFNE"
BASE COUNT          879 a         1479 c         1531 g          868 t
ORIGIN      
        1 cgcgatctcg ttgggcctcc tgtgggatgt ggtgttgcgg cgccctgcat gcgcgtttgc
       61 gcacgaaacc aatgccgccc taccttttcg cagactgagc ggtaccacgc ttccttctcc
      121 ggcaactttg cccctccacc tgctgccgag tatctttttg tctcccttcg ctgacagctt
      181 ccacatggaa aagggtctct cttgccaacg cgaatcgcca ttctgggcgc cattgtctac
      241 gacgaaatca tcacgcacga cggccagcgt atcgagtcgt tcggcggaat cgtgtataac
      301 atcgccgcgc tgtcgtcctt catcgaggac ggcgtcgtcc tgcccgtctc caacgtcggg
      361 ttcgaccggt acgatcaggt gctcgccctc atggccggca tggaccatgt ggatacgagc
      421 cagctcctcc gtaccgacac gccgtgcacc cacgcaaaac tgatctacca cgggtccaac
      481 taccgcgagg aaatcgtccg ccacatgatg ccgcctttca gcgccccgcg catcatctcg
      541 gcagtgaatg gagacgcatc cactcccgcc gctgacggaa tcctgatcaa ctttgtgaac
      601 ggcacggagc tggatcttcc gacgttgcag gctctccgga agcagacgaa ggcgccgctc
      661 tacctcgaca tgcacaacat catggtgcgg ttcgacgccg acgggaagaa gcagtatctc
      721 gatttcgtcg agtggccgga atgggtcgag caattcgatt tcgtgcagat gaacgaattc
      781 gaatgcgaga aagtcctgca cacctcgccc tcatcgccca aggaattcct ccacgccgcg
      841 cagacggtgc tggccgccgg tgcggcggcc gtcgtcatca ccatgggccc ggcgggcgtg
      901 gcgctggcac accgtcgtga ggggatcgag tacggctgca ttatcccggc ggtcaccgtg
      961 aagaacttcg tggatgccac cgggtgcggg gacgcgttca gcaccggcac gttctggcat
     1021 tatctccgca cgggcaatcc gctcaaagcc gccatggcag gcactctggt gggcgccctg
     1081 aactgcgaag tgcgcggcgt cggcaagctg gaacgcgcac gcaacgccga tcagcgcatc
     1141 gccgaggtat caccccaact tgccgaccgc gtggagcacg ggtggctcgg cgaccggctc
     1201 aactgaacca ttaacgtgct ggggaagcga atgaaacgcg cagaacccga aatggctgtc
     1261 cttgtccggc tcgatctttc cggaatcccg ttcggccccg tcctccggcc gatggaaccg
     1321 gttccggagg cggagcgcgg gctgttctcc acatgggaaa cgccgctttc gccgtggggc
     1381 gggtttgacg tcggtgccgg gcttgcctgc gtggacgacg ccggaagacc ggcgctcgag
     1441 tgtgccgaag gtgaacgcca tgagcgcgcg atcgttgcgc ggaaagtcga cctgcgcgac
     1501 ggcaccatcg ttgccgaggt gaaaccactc gctgcgaacg ctctcccgaa cgatgacagc
     1561 ccggttcaga ccgaagcgct ggccggcatc gtgttccgcg tgcagacctc gcgcgcgtac
     1621 tacctgttcg gcatccaggg gaaacgccgc gtggtgctgc tccgacggaa cgatgacgag
     1681 tttgccgtgc ttgcggaaca gtccatcgcc gccccgcagg actacgtgac gctggaagtc
     1741 cgcctcgacg gtgactccat ccgatgcacg tcaccggaac tcggcgtgga catgcacgtg
     1801 tgcgacaccg tgtatcacgc cggaaaggtg ggtttccgat cggccaactc ctcacgtctg
     1861 cggcggttcg aagtatctca gacccccggg cagcgccgcc gcgatgaatc acggcacgcg
     1921 cgcttcgaaa gagaagttgc cgaacggggc gcgggcattc ctgacgcaac ccttgtgcgc
     1981 acgtatcgca ctgccgatct cggcggaagt ccgctgttca gggccttcat cgaaccgggc
     2041 cgcttcgaca tccttgtgga aggcaagcag ctccgcgcgc tcacgcctga aggtggcctc
     2101 atctgggagg ttccggaaag gatcattcgg tgcgtcttct cgcgggattt tggccctgcg
     2161 gggcggctca tctatggctt cgcgggcaaa cgggaagaac gccgcgcgaa ggacatcgcc
     2221 ggcggcgaga gcgtgctcac ggttgaaaac gaaatggtgg tgattgaagg gcgaacggga
     2281 aagatcgttg cccgcgctga actgccgccc aacgtcccca cgcagcgctt ttttgactgg
     2341 tcaccgcgaa gtgcctcgct tgcgaacagc gacggcactg atattgtgct gcgtgagtgg
     2401 cgggatgacc acggtggcgg cggaatccgg ctctgggcgt acgaccgggg cctgaacctg
     2461 ctctgggagc acgtccagac cggtgcacac tacggtcatc actgggcgct ggatttcttc
     2521 gacgtggacg gagacgaccg cgaagagatg ctggccggcg gctgtctgta ccgcggcgac
     2581 ggcaccattc tgtggacgca tgaccgcgcg gacgaaatgc aacagatccg tggcgccggc
     2641 cactacgacg ccgtcgtgat gggcaacctc accggcgaag cggaggacga cccgatcgcg
     2701 ttcctgtgcg caggcagcgc cggtgtgtac gtggtggatg cccttaccgg cgccacccgg
     2761 gtggtgcacc gaatcgggca tgcgcaggga cggagtatcg gcaagttgcg gcccgacctg
     2821 ccgggcaccg aagtgctggc ggttacgaga tggggtaact acgggattct cacgctcttc
     2881 agcgggcgtg gtgaacgctt gtggactatt cagccggatt acgtcgggca gggcgcgacg
     2941 cccgtaaagt ggggcaaagc gggccaccgg ctcatctgga cgaacacttc gcgcgacgcg
     3001 cagctctttt tcgatggcta tggccgcctg gtgaaacggc tcccggagct gagtcgcgtg
     3061 tggggcgacc gcatgaaccg tgacgtcgga cggggtggca gccccgttcg catcgggact
     3121 gacccgaccg acctgctcac cctcacggtg ggaggggttg ttcatgtgtt cggtcctgcc
     3181 gagtagagcc tttcgcgcgg agcaacgtgg gagccgcgac atgacgtctg cgcgcaaagc
     3241 ggaaatggtc tctggctgac gcagaacgcc ggaaggtttg tccggagagg ggaattccgg
     3301 aaatttgagt agtgtgttcc aaccgcgcgt gccgtggttt tcacgatagc cgcaataacg
     3361 gccagagtcc tcttcgcggc ccctgctggc gagaaaccct tttcctcatg gagctgttca
     3421 tgctgcgcaa acattccgag acgatcgtca acaaggtgta tccggtccct gaaacgccga
     3481 cgtatctccc cggttcggtg atcgagatcg tgaacccgga tatcgaccgc ggccacatca
     3541 aacacgccgt gttcgatttc gacggcacgg tgagcgtgct gcgcgagggc tggccggata
     3601 tcatgctgcc cgtcatggtg gatgcgatcc ggggcgacca ccccgccaca cccgaaatcg
     3661 tcgagcggtg caagaagttc attgacgaga ctaccggtat ccagaccttc ttccagatgg
     3721 aagggctcgt gcacatggtc gaggagttcg gcctcgtgcc caaggacaaa atcctcgacg
     3781 cgtggggcta caaggctatt tacaacgagg ccctcatgaa acccgtgcgc gagcgcatcg
     3841 cggcgctgga acgcggcgag aagacgctgg aagacgtgac gatgttcgga tcgcgcgagt
     3901 tcctcaaggc gctgtacgat cgcggcgtgc gtatctacct cgccagtggt actgatcagg
     3961 aggacgtgcg caacgaagct cacgcgtgcc agattgatca gtgggccacg ggtggcatct
     4021 ggggcgctat caggaacgtg gatgactact ccaaagacaa agtgatgaag gacattatct
     4081 cccagaacaa cctgcacggc gcggaactga tggtggtggg ggatggtccc gtggagattc
     4141 gcaacgcgaa ggacaacggc gcgatcgcgg tgggcgtggc gtctgatgag gtagcacggc
     4201 gcggcctgaa cgcgcacaaa cgtcagcggc tcatcaaggc aggcgccgac atcgtcattc
     4261 ccgatttcct cgagtggaag aagctgctca agtacctgtt caacgagtag atcgctcgaa
     4321 ttcctgaccg ggtgatgggg caggctggtc cgcccagggc gggctcgata gcctgccccg
     4381 tttttttgcg cggagggagg cccccgcttc gtcgggtcct tccggtcagg cgctcccccc
     4441 ttcaaaccgc ggcctctgcc ggccgtcgag tggcgaactg gtcgctgagc ctcctctcgc
     4501 tgcgccgagg cccacgcggt ccctgagcct gtcgaagggc gcgcaggaag cccgccgaag
     4561 cgccagttcg ctgtatcgag acgcaccggc acgcggttcg ttgccagggg cgacaccaca
     4621 cagcctggct cgcagggccg ggcgagacac gcgttcttct tctttcgaat cctgccgtgc
     4681 gcatcgcacc tgtccgaccg gcccggtcat ctcgatacac cgtccgcacg cggacgccac
     4741 tcgatgaccg gttgtgt
//
LOCUS       DAOTNG010000251         4755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14765_length_4755_cov_5.077660, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000251 DAOTNG010000000
VERSION     DAOTNG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14765_length_4755_cov_5.077660"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="P5179_11185"
     CDS             <1..1324
                     /locus_tag="P5179_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95824.1"
                     /translation="PAPLVWHPPSTFDPDAPGLGFPTLDGVSHRVIFDPAPSKANVDE
                     GGDGKYESLLHGNFCHGPSFVVVGEHIVARWSNHTMDEGGPGARSIGRVGTFINDGDE
                     IDWGGQETFVELAPPPIPPRRRKFAVDPLNLWPYTGAGLSLVNGSLYALGLIHSVVGY
                     TDEEQYRATRGPVPAEHFRDALDVDAGFFFDKWGYHGLEWVQRWKIVKGRLLPDSPMY
                     RISPYEPRFEVTPGRFKIACPLQEPYCCALPIEEAPEALREDVSMLREEALIPKWRFT
                     AGEKWIAADGRHALAHLAEYVRPDGRSVMVRDNLLNPGHYYASLTDENGAYPPAVETN
                     LIGGANIAAGTLPDGRNFIIGNNYDDYYHAPDKSRKDMYISLSRDGVNFDRTYLILHL
                     DRKGDGGVYKYGGPQYYKPLIHKNNMWVFYSITKMKIGLTKIPLTAFA"
     gene            1370..2362
                     /locus_tag="P5179_11190"
     CDS             1370..2362
                     /locus_tag="P5179_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRS95825.1"
                     /translation="MEPVVAQDIPQTLAPDLALSMLRMILTIRMFEEKMLEFLNTGVI
                     KGGSHLYIGQEAVATGVCAALRKDDYITSTHRGHGHGLAKGGDPKALMAEILGKATGV
                     CRGRGGSLHLADLNTGNLGANGIVGGSLGIAAGAALSAKMRDSGQIVACFFGDGAVNT
                     GIFHEAMNMAKVWELPVLFVCENNLYGMSVSVNRATGLTDLADRADGYRMRKAIVDGQ
                     DVFAVWEATRHYGEIARSGGGPTFIECKTYRFGGHSRSDARKYRTREEERKWRERDPV
                     TVCAQRGIEEGWFDAGTVERIRSEVAAIIEEAADFALSSPEPDPSELTDDLYAA"
     gene            2419..3768
                     /locus_tag="P5179_11195"
     CDS             2419..3768
                     /locus_tag="P5179_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95826.1"
                     /translation="MAKPNILLFMMDALRPDRLSPWGHDAVAVGPPHPTPNFARLAET
                     GVFFRNCFSHMPSSHPSRASILTGRDPHTHGVRINSRPLSASETTLTQILRNAGYLTA
                     TTRTFPPGMGRGFEERGLVSKVLSPFDAGPWDIVKELPQMAEDEAGTDVAQDTTALIR
                     WLQRYVADPERRKHPFFLWADCEDTHEPWRPPVPYNTMYDPEPYDGPDVSCPPMYSPH
                     LTERQVRHARALYDGVVALEDAHLGRLLDALERLGLAQDTLLIVMSDHGVHLGENHLW
                     RKPPTLFDAVLRATLIMRLPGVIPQGRHTDGLNLVNDVFATVLDLAELEMPSGAVGQC
                     TSLRPLWEGQQQVRNFVPLEFNLYKGTAGKGIRTERWKYFYYRSVGDLMWDTMSPADV
                     WRGEGWPLTMLFDLENDPAESRNVADEFPQVEREMRDILLNWLIDSENDLPAPVPGD"
     gene            3873..4523
                     /locus_tag="P5179_11200"
     CDS             3873..4523
                     /locus_tag="P5179_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRS95827.1"
                     /translation="MRWKEVITDTAVTASLRMEALLSAHTLVLNRHWMAVGFTQARRA
                     ISLVYEDVAKVIAPDTFALYDFDTWADLSRHAPTQDCVRSVRVVFRIPEIIILTTYSG
                     MPRANVAFSRRNLCIRDRYTCQFCGEKLSSENLTIDHILPRSRGGHATWENCVLACVR
                     CNERKGDRMPEEVGMRLLKQPRRPRWAPSFGIPLRHRKESWEKFISDVYWDIELES"
     gene            4538..>4755
                     /locus_tag="P5179_11205"
     CDS             4538..>4755
                     /locus_tag="P5179_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRS95828.1"
                     /translation="MTDAHEHRLGWDTYFMRIAQLVAERSTCTRAKIGAVIVRERSII
                     ATGYNGSPAGMPHCTEVGCLVYRSTAPDG"
BASE COUNT         1004 a         1366 c         1467 g          918 t
ORIGIN      
        1 cccagctccg cttgtctggc atcctccttc cacattcgac cccgacgcac cgggacttgg
       61 cttcccaaca ctcgatggag tatcacatcg cgtgattttc gacccggcac catccaaagc
      121 caacgtggac gaaggtgggg atggcaagta tgaaagcctc ctgcacggca atttctgtca
      181 tgggcccagt ttcgtggtgg tgggtgagca catagtcgcg cggtggtcga accacacgat
      241 ggatgaaggc gggccgggcg cgcgttccat cgggcgcgtg ggaacgttta tcaatgacgg
      301 cgacgagatc gactggggcg gccaggagac gttcgtggaa ctggcgcctc caccgattcc
      361 cccgcgccgc cggaagttcg ccgttgaccc gctcaatcta tggccgtaca ccggtgccgg
      421 tttgtcgctg gtcaacggct cactttacgc cttgggatta atccactcgg tggttggtta
      481 cacggatgaa gagcagtaca gggcaacgcg cggcccggtc cccgcggaac atttcaggga
      541 cgcgctggac gtcgacgcgg gtttcttctt tgacaagtgg ggctaccacg gacttgagtg
      601 ggtgcagcgc tggaaaatag tgaaaggacg gcttctacca gactccccga tgtaccgcat
      661 cagcccctac gaaccgcgtt ttgaggttac tccagggcgg ttcaagatag cctgtccgct
      721 gcaggaaccg tattgctgcg ctttgccgat tgaggaggcg ccggaggcgt tgcgcgagga
      781 tgtgagcatg ttaagagagg aagcgctcat accaaagtgg cgcttcaccg cgggcgagaa
      841 atggattgcg gccgatggca ggcatgcact ggcacatttg gcggagtacg ttcgtcccga
      901 tggcagaagc gtcatggtcc gcgataatct gctcaacccg gggcattact acgcctccct
      961 taccgatgaa aatggcgcgt acccccctgc ggtggaaacc aaccttatcg gcggcgcgaa
     1021 cattgcggcg ggaacgttac ccgatggaag gaacttcatc atcgggaaca attacgatga
     1081 ttattaccat gcgccggaca aaagcaggaa agacatgtat atctctcttt cccgggacgg
     1141 cgtaaacttc gacaggacat accttatcct tcatctggac aggaaaggcg atggaggagt
     1201 ttacaaatac ggcggcccgc aatactacaa gcctttgatt cacaagaata acatgtgggt
     1261 gttctacagc atcacgaaaa tgaagattgg tcttacgaag attccgttga ctgcgttcgc
     1321 gtaatgacgg aggagtcggt tggaacaaac ccggcgacca gggaagcaca tggagcccgt
     1381 tgtggcacag gacatacctc aaactctcgc acctgatctc gcgctgtcga tgctccgcat
     1441 gattctcaca atacgcatgt tcgaggagaa gatgctggag ttcctgaaca ccggagtgat
     1501 caaaggaggg tcccacctgt acatcggcca ggaggcggtt gcgacaggcg tgtgcgcggc
     1561 attgcgcaag gacgactaca tcacaagcac tcatcgcggt cacggccacg gacttgcgaa
     1621 gggaggcgac ccgaaagccc tcatggcgga aatcctcgga aaagcgaccg gagtatgtcg
     1681 gggtcgcggc gggagccttc atctcgcgga cctcaacact ggcaacctgg gggcaaatgg
     1741 catcgtgggc gggtcactcg gtattgcggc aggagctgct ctttcggcga aaatgaggga
     1801 ttccgggcaa atcgtggcct gcttcttcgg cgacggtgct gtgaatacgg gaatcttcca
     1861 cgaagcgatg aacatggcga aggtgtggga gcttccggtg ctgtttgtgt gcgaaaacaa
     1921 cctctacgga atgtccgtga gcgtcaacag ggcgaccgga ttgacggacc tcgcagatcg
     1981 ggcggacggg taccgcatgc ggaaggcgat tgtggacggg caggacgtgt tcgccgtgtg
     2041 ggaagctacc cgtcattacg gggagatcgc gcgatccgga ggtggcccta ccttcatcga
     2101 gtgcaaaacc taccgctttg gcgggcattc ccgttctgac gcaaggaagt accggacgcg
     2161 ggaagaagag cgcaaatggc gcgaacggga cccggtcact gtgtgcgcgc agcgcggcat
     2221 tgaagaagga tggtttgacg cgggtaccgt cgaacggatt cggtctgagg tggcggcaat
     2281 cattgaagaa gccgccgatt tcgccctgtc cagcccggaa ccggacccca gcgaactcac
     2341 cgacgatctc tacgccgcgt agcgatctgt tccccacaac gacagcattt tttgaacctt
     2401 acagggagat ccggattcat ggcaaagccc aatattctcc tgttcatgat ggacgcattg
     2461 cgccctgaca ggctcagccc gtgggggcac gatgccgtcg cggtcggtcc accccaccca
     2521 accccgaatt tcgctcgact tgccgagact ggcgttttct ttcgcaattg cttttcgcac
     2581 atgccatcga gccatccgtc cagggcaagt atactgacag ggcgtgaccc ccatacccat
     2641 ggcgtacgaa tcaattcccg accattgtcc gcctcagaaa ccacgctgac gcagatcctg
     2701 cgtaacgccg ggtatttgac ggccaccacc cggacgttcc cccccggcat gggacgggga
     2761 tttgaggagc ggggcctcgt ctccaaggtg ctttcgccgt tcgacgcggg gccatgggac
     2821 atcgtgaaag agctcccgca gatggcggaa gacgaagcag gcacggatgt ggcgcaggac
     2881 accaccgccc tcatcaggtg gctgcaacgt tatgtcgccg atccggaacg gcggaaacac
     2941 ccgttctttc tgtgggcgga ctgtgaagac acccacgaac cctggcgccc gccggtgccg
     3001 tacaacacga tgtacgatcc tgaaccctac gacggaccgg acgtgagttg cccaccgatg
     3061 tattcgccgc accttacgga acggcaggtc agacatgcgc gggcactgta cgatggcgta
     3121 gtcgcgctgg aggacgccca cctcgggcgc cttctcgatg cgctggaaag gctgggcctc
     3181 gcgcaggaca cgctgttgat cgtcatgagc gatcacggtg ttcatctggg cgaaaaccat
     3241 ctgtggcgca aaccgccaac gctgttcgac gcagttctcc gggcaacact catcatgcgc
     3301 ctgccgggcg tgattccgca gggccggcac accgatggtc tcaacctcgt caacgatgta
     3361 ttcgcgacgg tactcgacct cgctgaattg gagatgccct cgggggcggt gggtcagtgc
     3421 acgagccttc ggcctctgtg ggaagggcag caacaggtaa gaaactttgt accgctcgag
     3481 ttcaatctgt acaaaggaac cgctggcaag ggcatccgca ccgaacggtg gaagtatttt
     3541 tattaccgat ctgtcggcga cctgatgtgg gataccatgt ccccggcgga tgtatggcgc
     3601 ggagaagggt ggccgttgac gatgttgttc gacctcgaaa acgaccccgc agaatcgcgg
     3661 aatgtggccg acgaattccc gcaggtcgag cgggagatgc gggacattct gcttaactgg
     3721 ctcatagact cggaaaacga cctccccgcg ccggtgccgg gagattagaa cctccctgca
     3781 tttgacacga ggcgagccac cgggctggcg cacgcggggt gtgtccgttt acatttcaag
     3841 ttctgaagca tcgggttcgc agtatttcct ccatgcgctg gaaggaggtg atcaccgaca
     3901 ccgcggtaac tgcttccctg cgaatggaag cgttgctctc cgcgcacacg ctggtgttga
     3961 accgtcactg gatggcggta ggtttcacgc aagcgcgccg cgcaatcagc ctcgtgtacg
     4021 aggacgtggc caaagtcatc gcgcccgaca cctttgcgct gtatgacttt gacacctggg
     4081 cggacctctc ccggcacgcg cctacgcagg actgtgtccg gtccgtgcgc gtggtgttcc
     4141 gaatccccga aatcatcatc ctgaccacct acagcggcat gccgcgagcc aacgttgcct
     4201 tctcccgccg gaatctttgc atacgcgacc gttacacctg tcagttctgc ggcgagaagc
     4261 tctccagcga aaacctcacg atcgaccata ttctgccgcg ttcgcggggt ggtcacgcca
     4321 cgtgggagaa ctgcgtgctg gcgtgcgtcc gatgcaacga acgcaaagga gaccggatgc
     4381 cggaagaagt agggatgcgg cttctgaaac agccgcgacg gcccaggtgg gcaccttcat
     4441 tcggaattcc gcttcgccac cggaaagaga gctgggagaa gttcatctcc gacgtctact
     4501 gggatataga actggagtca taggataggg ttcgcggatg actgacgcgc acgaacacag
     4561 gctcgggtgg gacacgtact tcatgcggat cgcgcaactg gtggccgagc gttccacctg
     4621 cacgcgggcg aaaatcggtg cggtaatcgt gcgggaacgg agcattattg cgactgggta
     4681 caatggttct ccggctggaa tgccgcactg cacggaggtc ggttgcctcg tgtaccgctc
     4741 gaccgcgccg gacgg
//
LOCUS       DAOTNG010000252         4732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_14854_length_4732_cov_4.862091, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000252 DAOTNG010000000
VERSION     DAOTNG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14854_length_4732_cov_4.862091"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1265)
                     /locus_tag="P5179_11210"
     CDS             complement(9..1265)
                     /locus_tag="P5179_11210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS95829.1"
                     /translation="MFSRDDCRRNLRWYILFRMSLDFMMWGPTWVLYMRDELGFSFTE
                     IMLFDFFFQTAIVLLEVPTGMFADIIGRRWALRGGAFSLFLAMGMWGAGWNAWWWISI
                     AWAFWAIAATLINGADASLVYETLRADGRGYEFGKVLGRISSLSMLGMVAASVLGGWM
                     AEYGYRVPILVHTALLAFTVFAAFRLREPPRAERLVHATVRNIARSIRALLKRSHRFR
                     YLLIYSALIQVVEIMAVVYQQPLLVSLGFPIKQLGVFYAGAILIASVGPIAMTWASDR
                     FGIARTLGLGALGTTIACLGLFWLPGLTIVIPFLVLRFFADGVRPVTIEGMNRLADPD
                     DRATVLSLRSICISSVAGPMEVLSGWWADRVPIRRVYLVCGALLPLFSVWLGWAWKRH
                     PEPMPKDVQAVPPVPRLGGDPSLHSE"
     gene            complement(1302..2096)
                     /locus_tag="P5179_11215"
     CDS             complement(1302..2096)
                     /locus_tag="P5179_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS95830.1"
                     /translation="MRGADLLSLRFARGTGHLVLASVRQVGRFVLFASEVVRALPRIF
                     QNPRLTLEQMVRMGTDSIPLVFTVSIFAGATTAWQGKYQLEGILPTAAIGTAVAKSLI
                     LELGPVLTALVIAGRVGSSIAAELATMRVTEQIDALVTMSISPARYLIAPRVVAGFVM
                     VPVLVVMSYFFGISGGYVTAVSLGGLNGARFLEGLRLFFYVRDVAVGLLKAFVFGAST
                     SFLGCYFGYYARGGAEGVGQAAIRAFVSSSVVILGADFLIASIAFM"
     gene            complement(2096..3535)
                     /gene="dnaB"
                     /locus_tag="P5179_11220"
     CDS             complement(2096..3535)
                     /gene="dnaB"
                     /locus_tag="P5179_11220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRS95831.1"
                     /translation="MAGRIEGPAQEAMEELASAGAERVLPHSILLEQQVLGAMMLNRQ
                     AVSKVVSMLDRNAFYRGRHRLVFDACVRLYEADQPADLPLVIQELTRKGELAEAGGAE
                     YVADIAASVGTSTNVEYHAQQILELAVRRRTIALSSELINSAYDTTGTIYDNLERFEN
                     DLFKLAENRWREGFSHIEPIMHRTYERIEKVSTRKGEVTGVPSGFAALDEMTAGFQKS
                     ELIIVAARPGMGKTALVLNMARNAALQHKLPVGIFSLEMSAESVAQRLLCAEAHVDGQ
                     LVRRGRLNEHMWNRLSKAVTTLANAPIYIDDSAALSPLEVRAKARRLVHEHSVQLIIV
                     DYLQLMRVPGHREGRAQEVAVISQALKALAKDLDIPIIACSQVTREVEKRGEFRPRLS
                     DLRESGSIEQDADVVIFVHRPEYYKITHDEQGRNVEGLAEILLEKQRNGPTGKIELRW
                     TDKFGLFETYRRDDDEFATADVEEDGELY"
     gene            complement(3548..4429)
                     /locus_tag="P5179_11225"
     CDS             complement(3548..4429)
                     /locus_tag="P5179_11225"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRS95832.1"
                     /translation="MDRAESRRETLQLLRRFVEERRELGDGFWPRPEGAAIPAKQGTR
                     AAPPTAARPPVSAERFLIPEKKPEHETAYHPATEQPAIAGESDLAGRLFASAPEGAVP
                     DAASLEEFRARISECHKCPLGATRTHFVFGSGNPDADLMFVGEAPGEEEDLQGQPFVG
                     RAGQLLTKMIEAMKLRREDVYIANVLKCRPPNNRTPLPSEMEQCKPYLDRQIALIRPK
                     IICALGRVAAQALLNTGEGLNRLRGKFHDYRGVKVLVTYHPAALLRNPQWKPLSWDDL
                     QKIRYELDGTILRPKKA"
     gene            complement(4434..>4732)
                     /locus_tag="P5179_11230"
     CDS             complement(4434..>4732)
                     /locus_tag="P5179_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRS95833.1"
                     /translation="RKKKPGQIVVGFAVETEAEKADKRALERLQKKSLDMIALNVIGE
                     KGVGFGTDTNRVTLFCRDGSKSELPLMEKREIARIMWERIAALQGTGPQTKNQA"
BASE COUNT          912 a         1539 c         1395 g          886 t
ORIGIN      
        1 cgctctcgtc actccgagtg aagcgacggg tctcccccca gccggggaac cgggggtacc
       61 gcctgcacgt ccttcggcat aggctccggg tgccgtttcc acgcccatcc gagccacacg
      121 gagaagagcg gcagcagcgc cccgcacacg aggtataccc ggcgaatcgg caccctgtcc
      181 gcccaccacc ccgacagcac ttccatcggc ccggcaacgc tggagatgca gatgctccgc
      241 aacgagagca cggtcgcccg atcgtccggg tccgccagcc ggttcatgcc ctcgatggtc
      301 accgggcgca cgccatcggc gaagaagcgg aggacgagga acgggatgac gatcgtaagc
      361 cccggcagcc agaacagccc cagacaggcg attgtggtgc ccagcgcacc aagccccagc
      421 gtgcgcgcga ttccgaaacg gtcgctggcc catgtcatgg cgatcggccc cacggacgca
      481 atgagaattg cacccgcgta gaacacgccg agttgcttga tcgggaaacc gagggacacc
      541 agaagcggct gctggtacac caccgccatg atctccacga cctgaataag tgcagagtaa
      601 atcagcaggt agcggaaccg gtggctgcgt ttgaggagtg cacggatgga acgggcaata
      661 tttcgcaccg tcgcatggac aagccgctcg gcgcggggcg gctcccgaag gcggaacgcc
      721 gcgaacacgg tgaatgccag cagcgcggtg tggacgagga tcggcacgcg gtagccgtat
      781 tccgccatcc atccgccaag aaccgacgcg gcaaccatgc caagcatcga aaggctcgag
      841 atgcgcccga ggaccttccc gaattcatac ccccgcccgt ccgcgcgcag ggtttcgtac
      901 acgagcgacg cgtccgcccc gttgatgagg gtggcggcaa ttgcccagaa tgcccacgcg
      961 atgctgatcc accaccaggc gttccagccc gcgccccaca ttcccatggc gagaaagagg
     1021 ctgaatgcgc cgccgcggag tgcccaccgg cggccgatga tatcagcgaa catgccggtc
     1081 ggcacttcca gcagcacgat tgccgtctgg aagaagaagt cgaacagcat gatttcggtg
     1141 aaggagaacc cgagttcgtc gcgcatgtag agcacccagg tggggcccca catcatgaag
     1201 tcgagcgaca tgcggaaaag gatgtaccac ctgaggttgc ggcggcagtc atcgcgggag
     1261 aacatgcgta ttccgagggg gaaaaggggt ggagacgatg gttacatgaa tgcaatcgaa
     1321 gcgatgagga aatcggctcc cagaatgacg acggaagagg acacaaacgc cctgatcgcg
     1381 gcctggccta cgccttcggc tccacctctg gcgtaatacc cgaagtaaca cccgagaaac
     1441 gacgtggatg cgccgaacac gaacgctttc aggagcccga ccgccacgtc acgcacgtaa
     1501 aagaacaggc gcagcccttc aagaaacctt gccccgttca gccctccgag cgacaccgcg
     1561 gtgacgtagc cgcccgaaat gccgaagaag tagctcataa cgaccagtac cggaaccatc
     1621 acgaacccgg cgaccacgcg gggagcaatg agataccgcg cggggcttat ggacatggtg
     1681 accagtgcgt ctatctgctc cgttacccgc atcgtggcaa gttccgcggc gatggaagac
     1741 cccacgcgcc cggcgatgac aagcgccgtg agcaccgggc ccagctcaag aatgaggctt
     1801 ttcgccaccg ccgtaccgat tgccgcggtg ggcaggatgc cttcaagttg gtacttcccc
     1861 tgccatgccg tcgtcgcgcc ggcaaagatg gacaccgtaa acacgagcgg aatggaatcc
     1921 gttcccatgc gcaccatctg ctcgagcgtc aggcgcggat tctggaagat tctgggcagc
     1981 gcacgcacga cttcggaggc aaacagcacg aaccttccca cctgtcgcac ggaggcaagc
     2041 acgagatgcc ctgtgccgcg cgcgaaacga agtgagagca ggtcggcccc ccgcatcagt
     2101 agagctcccc gtcttcttcg acgtctgccg tggcgaactc gtcgtcgtca cgccggtagg
     2161 tttcaaaaag gccgaatttg tccgtccacc gcagctcgat tttccccgtc gggccgtttc
     2221 gctgtttctc cagcaggatt tccgccagtc cttcaacgtt ccggccctgt tcatcgtgcg
     2281 tgatcttgta gtattcgggg cgatggacaa aaatgacgac gtccgcatcc tgttcgatgg
     2341 atccactttc ccgcagatcg gaaaggcgcg gccggaactc cccgcgcttt tccacttcgc
     2401 gcgtgacctg agagcaggcg atgatcggaa tgtccagatc cttcgcaagc gctttcagtg
     2461 cctgggagat gacggcgact tcctgggcgc gcccttccct gtgcccggga acgcgcataa
     2521 gctgcaggta gtcgacgatg atcaactgga cgctgtgttc gtggacgagt ctgcgggcct
     2581 ttgcgcgcac ttccagaggg gacagggcgg cgctgtcgtc aatgtagatc ggcgcgttcg
     2641 caagggtcgt aacggctttg gaaagccggt tccacatgtg ctcgttcagt ctgccgcggc
     2701 gcaccagttg gccgtccacg tgcgcctcgg cgcagagaag ccgctgcgcc acggattccg
     2761 cggacatttc cagggagaag atgccgacgg gcagtttgtg ctggagagcc gcgttccgcg
     2821 ccatgttgag cacgagtgcg gtttttccca tacccggacg cgccgcgacg atgatcaatt
     2881 cgctcttctg aaatccggcg gtcatctcat caagcgcggc aaacccgctc ggaacccccg
     2941 taacttctcc cttgcgcgtg ctgaccttct cgatccgttc atacgtccgg tgcatgatcg
     3001 gttcgatatg ggagaatccc tccctccagc ggttttccgc cagcttgaac aggtcgtttt
     3061 cgaacctctc gaggttgtcg tagattgtcc cggtggtatc gtacgcggaa ttgatcagtt
     3121 cgctggaaag cgcgatcgta cgccggcgga ccgccagttc cagaatctgt tgcgcgtggt
     3181 attccacgtt cgtgcttgta ccgacgctgg cggcgatgtc ggccacatac tccgcgccgc
     3241 cggcttccgc aagctcgcct ttccgtgtca gttcctgaat caccagcggc aggtcggccg
     3301 gctgatcggc ttcgtacagg cgcacacacg catcgaacac gagacggtgc cggccccggt
     3361 aaaatgcgtt ccgatccagc atggaaacga ccttgctcac cgcctgccgg ttaagcatca
     3421 tcgcgccgag aacctgctgt tcgagcagaa tggaatgggg cagcacccgc tccgcacccg
     3481 cgctggcgag ttcttccatt gcctcctgcg caggaccttc aatccggccc gccatgctct
     3541 cctctcctca cgccttcttc ggccgcaaaa tggtcccgtc caactcgtat ctgattttct
     3601 gcaggtcgtc ccacgacagc ggcttccact gcgggtttcg caacaatgcg gcgggatggt
     3661 acgtgaccag aaccttgacg ccgcggtaat cgtggaactt acccctcaga cggttcaacc
     3721 cctcgccggt gttcagcagc gcctgcgccg ccacccggcc cagtgcacag ataatcttcg
     3781 ggcggatcaa cgcgatctgc ctgtccagat aaggcttgca ctgctccatc tcgctcggca
     3841 ggggagtccg gttattcgga gggcggcatt tcagcacgtt ggcgatatac acgtcctcac
     3901 gcctgagctt catcgcttca atcatttttg tcagtaattg ccccgcacgc ccgacaaatg
     3961 gttgcccctg aaggtcttct tcttctccgg gtgcctcccc gacaaacatg aggtcggcat
     4021 cggggttgcc cgatccgaac acgaaatgcg tacgcgttgc gccaagcggg catttgtggc
     4081 attcactgat gcgcgcgcgg aactcctcca gcgatgctgc gtccggaacc gcgccctccg
     4141 gcgcggaagc gaacaggcgg ccggcgaggt cgctctctcc tgcgatcgcc ggctgttctg
     4201 tcgcagggtg gtacgcggtc tcgtgttccg gctttttctc cggtatcagg aaacgctccg
     4261 cggataccgg cggtcgagcc gcagtcggcg gagcagcacg tgttccctgc tttgcaggga
     4321 tcgctgcacc ttcgggtctg ggccagaatc cgtccccgag ttcgcggcgt tcctccacga
     4381 agcggcggag caactgcaac gtctctctgc gtgattcggc gcgatccatg aacttacgcc
     4441 tggtttttcg tctgcggacc cgtgccctga agcgccgcga tccgttccca cattatccgc
     4501 gcgatctctc gcttttccat caacgggagt tcggatttcg acccgtcccg gcagaacaat
     4561 gtcactctgt tcgtatcggt gccgaagcct acgcctttct cgccgatgac attcagtgcg
     4621 atcatgtcga gggacttctt ctgcaggcgt tccagcgccc gcttatccgc cttttcggcc
     4681 tcggtttcca cggcgaagcc gaccacaatc tgccccggtt tcttcttcct gg
//
LOCUS       DAOTNG010000253         4675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15089_length_4675_cov_5.006277, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000253 DAOTNG010000000
VERSION     DAOTNG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15089_length_4675_cov_5.006277"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="P5179_11235"
     CDS             <1..1274
                     /locus_tag="P5179_11235"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS95834.1"
                     /translation="EDVFFSPPFSCHLLPSAGVPMCSASSRSTRPLAATAGYYGAFVA
                     MGLMTAAVGPTLPGLAEQTGSTLRAISYLFSARSLGYLAGSFSAGHAYDRYPGHPILA
                     GAVVFMALMALAVPFMPAIWLLTIAMVGLGFGEGFLDVGGNAMLVWQHGARVGPFMNG
                     LHFCFGLGAFVAPIVIAQVIGITGGIRWAYWVLGGVAWLPAVWILSVPSPVIRKPADA
                     SVPGERNDRLLGLIVLFFFLYVGAEAGFAGWIYTYALSLKLATATGAAYLTSVFWGSF
                     TLGRLLAIPISAKVAPRTILVTDLAASICGVSAILIWQTSTIVVWAGTFVVGLSLASL
                     FPTTVNLAEQRMVLNGRVTSWFFIGASAGGMCVPWLIGQVFEQRGAVITMVMILCDLA
                     LTASVFLLMIRQIASATVRNTSATTSVSPEV"
     gene            1278..2693
                     /locus_tag="P5179_11240"
     CDS             1278..2693
                     /locus_tag="P5179_11240"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRS95835.1"
                     /translation="MPAGLVMVWFRSDLRLNDNPALHFAASTGKSVLPVFVWNAPREI
                     PWAQGRASKWWLHESLVSLDASLRQKGARLVIRDGNPGMALATLAAETGADTVVWNST
                     REPDGRETDAELERQLRRAGISVKVYRSAFLFSPDNIRTREGKPFLVFTPFWNACRAE
                     KPPREPLPAPARIPFPEVPVSSRSLDELGLIPAPDRTEGIRAVWKPGEPTAQKMLHEF
                     LVTRVDAYKTDREIPSIRGTSRLSAYLHFGEISPHTVWHHAFALNAEHFLRQLCWREF
                     ASHLLFHFPHAADHPLKTEYNRFPWNEDTAALRAWQTGATGFPFVDAGMRELWATGWQ
                     HNRVRMVTASFLTKDLLLNWTLGARWFWETLVDADLANNTLGWQWVAGCGADAAPYYR
                     IFNPVLQGEKFDPKGEYVRRWIPELAGVSDRFIHRPWEAPAGELRAAGVKLGSTYPSP
                     IIHHEEARKRALSAYASLRRP"
     gene            2714..>4675
                     /locus_tag="P5179_11245"
     CDS             2714..>4675
                     /locus_tag="P5179_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRS95836.1"
                     /translation="MATSRHSIVSRQPAIRWQDAFPSGNGCIGALVYGSIAEERILFN
                     HEHLWLRIPKPELPDVSAHLPELRRLLEQGRYQDAQLFLSKRLEEAGYPHRRPAPYHP
                     AFDLALRMPTREAFRRYRRSLDFETGEVRVEWEDGGCAFQRDLFVSRADDVVVYRIRG
                     ERHFSGSVALVPHESTPSAPPSVTFHSSAEDGWLTFVGTHAERDLYPEGNQFGGIARV
                     DISGGTASVTGNVITFTDAAEVLFRVGLFANEMAEAAIPEARSRIAALPESYEALKQR
                     HVELHRPLFLRTDITLDTAPDERSTEELLLDAYDGTVPVRLIETMFDFGRYLLISSSR
                     PGGLPANLQGIWNGEWNPPWQSDFHNDENIQMNYWQALPGSLPETLEPFFSYFESMVP
                     DYRENARKVYGCRGILLPICQTTDGMLYTDVWTNWTAGAGWIAQHFFDYWLFTGDKEF
                     LLRRAIPFMKEVALFYEDFLFEGPDGRLVFSPSLSPENIPDRPGASHAQINATMDVAV
                     ARELLTNLCSACELMDVECEGVARWRKLLEKLPAYETNKDGAIREWIHPDLPDNYHHR
                     HLSHIYPLFPGLEVTRETEPELFEACRVAVEKRLVIGLSSQSGWSYAHMANIYARLGN
                     GDRALDCLENLCRSVVGPNLFTYHNDWRQQ"
BASE COUNT          886 a         1393 c         1411 g          985 t
ORIGIN      
        1 ctgaagatgt tttcttttct cctccgtttt cgtgtcacct ccttccttct gcaggcgttc
       61 ccatgtgctc ggcttcgtcc cgatcaaccc gtccgctggc tgcaacggca gggtattacg
      121 gggcgttcgt cgcaatgggg ttgatgactg cggcagtcgg gccgacacta cccgggctcg
      181 ccgaacagac cgggagcacc ctccgcgcaa tcagttacct tttctcggcg cgatcgctgg
      241 ggtatctcgc cggttcgttc tccgccgggc atgcgtacga tcggtacccc ggacacccga
      301 tactggcggg tgccgtcgta ttcatggcac tgatggccct cgctgttccc ttcatgcccg
      361 caatctggct tctcaccatc gctatggttg gccttggatt cggtgagggt ttcctggacg
      421 tcggcggaaa tgcgatgctc gtgtggcaac acggggccag ggtcggaccg ttcatgaatg
      481 gcctgcactt ctgtttcggg ctcggcgcat tcgtcgcccc gatcgtgatc gcgcaggtga
      541 tcggtatcac cggaggcatc cgctgggcat actgggtatt gggcggagta gcgtggcttc
      601 cagcagtgtg gattctcagc gtcccgagcc ccgtgattcg caagcccgcg gacgcgagcg
      661 taccaggaga aaggaacgat cggcttctcg gcctgatcgt ccttttcttc tttctgtacg
      721 tcggagccga agccggtttc gcgggctgga tctacacgta cgcgctttcc ctgaaacttg
      781 ccactgccac gggtgcggca taccttacgt cggtgttctg gggctcgttc accctcggcc
      841 gacttctggc aatcccaatc agcgcaaagg tcgctccgcg cacgatcctc gtaaccgacc
      901 tcgcagcctc gatttgcggg gtcagcgcca tactaatctg gcagacatca accatcgtgg
      961 tgtgggcagg tacgttcgtg gtcgggctct cgttagcttc cctgttcccg actacggtga
     1021 atcttgcgga gcagcgaatg gttctgaatg gtcgggtaac cagctggttt ttcatcgggg
     1081 caagcgcggg agggatgtgc gttccatggt tgatcgggca ggttttcgaa cagaggggcg
     1141 ccgttatcac gatggtgatg attctgtgtg atctggccct gacagcgagt gtattcctgt
     1201 tgatgatccg ccagatcgct tcagccaccg tcagaaacac gtcggccaca acgagcgtga
     1261 gtccggaggt gtgagctgtg cctgctggcc ttgtgatggt atggttccgg tctgatttgc
     1321 gcctcaacga caacccggcc ctccacttcg ccgcatccac cggaaaatcg gttttgccgg
     1381 ttttcgtgtg gaacgcgcca cgagaaatcc cctgggcgca gggaagggcc agcaagtggt
     1441 ggttacacga atccctggtc tcacttgacg cgtcgctccg gcagaaggga gcgcggctgg
     1501 tcattcgcga tgggaatccg ggtatggctc tggccaccct tgcggcggaa acaggggcag
     1561 acacggtcgt atggaattcc acaagggagc cggatggacg ggaaaccgac gccgaattgg
     1621 agcgccagct tcggcgcgcg ggaatttcgg tcaaagtcta ccggtcggcg tttctttttt
     1681 cgcccgacaa tatccggaca cgagagggca aaccattcct cgtattcact ccgttctgga
     1741 acgcgtgccg tgcggagaaa ccgccacgag aacctctgcc cgcgcctgcg cggataccat
     1801 ttcccgaagt accggtcagt tcgcgttcgc tcgacgaact tggcctgatt cccgcgccgg
     1861 atcgcacgga aggaattcgt gccgtatgga agccgggtga accaactgcg cagaaaatgc
     1921 tccacgagtt cctggttacg cgcgtagatg cgtacaagac ggacagggaa atcccaagca
     1981 ttcgcgggac gtctcgcctt tcggcgtacc tccatttcgg cgaaatcagc ccgcacacgg
     2041 tatggcacca cgcatttgcg ctcaacgcgg agcacttcct ccgtcagctc tgctggcggg
     2101 aattcgcgtc tcacctgctc tttcatttcc cgcacgccgc ggatcatccc ctgaaaacag
     2161 aatacaaccg gttcccctgg aacgaagaca ctgcagccct gcgcgcctgg caaaccggcg
     2221 caaccggatt tccgtttgtc gatgcgggga tgagagagct ttgggcaaca ggatggcagc
     2281 acaatcgcgt gcgcatggtt actgcatcgt ttctcaccaa agaccttctc ctgaactgga
     2341 cgctcggcgc tcgctggttc tgggaaaccc tggtggatgc tgatctcgcg aacaacacgc
     2401 ttggctggca atgggtggcc gggtgcggtg cggatgctgc cccgtattac cgaatcttca
     2461 acccggtgct gcagggcgag aaattcgatc cgaagggcga gtacgtccgc cgctggatac
     2521 cggaacttgc gggagtatcc gacaggttca tccacaggcc atgggaggca ccggcgggtg
     2581 aactccgcgc cgcaggcgtg aaactcggtt ccacgtatcc ctcccccatc attcaccacg
     2641 aagaagcacg caagcgggct ctttcggcgt acgcgtcgct caggcgcccg tagattgtcg
     2701 gaaagggatt tccatggcaa catccaggca ttcgatcgtc tcgcggcaac cggcaattcg
     2761 ttggcaggat gcgttccctt ccggaaacgg ctgtatcggg gccctcgtct acggatcaat
     2821 cgcggaagaa cgcatccttt tcaatcacga acatctctgg ttgcggattc caaaaccgga
     2881 gcttcccgac gtttcagccc atcttcccga attgcgaaga ttgttggaac agggacgata
     2941 ccaggatgca cagctttttc tcagcaagcg actcgaggag gcggggtacc cgcaccggcg
     3001 cccggcaccg taccatccgg ccttcgatct cgcgctccga atgccgactc gtgaggcgtt
     3061 ccgacgatac cgccggtccc tcgattttga aaccggcgaa gtccgagtgg aatgggaaga
     3121 tggcggctgt gccttccaaa gggacttgtt cgtgtcgcgc gcggacgatg tggtagtata
     3181 caggattcgc ggtgagagac acttttcggg cagcgttgcg ctcgtgcctc acgagagcac
     3241 accctctgcg ccgccgtcgg tgacattcca ttcctccgcc gaggacggat ggttgacgtt
     3301 tgtggggacc cacgcggaaa gggacctgta ccccgaaggg aatcagttcg gcggaatagc
     3361 ccgggtcgac atttctggcg gaaccgcctc ggtcacaggg aacgtcatca cgttcacaga
     3421 cgccgcagaa gtgctcttcc gggttgggct gtttgccaac gaaatggcgg aagcagcaat
     3481 cccggaagcc aggtcgcgaa ttgcggcgct tcctgaatcg tatgaagccc tcaaacaacg
     3541 ccacgtggag ctccatcgtc ccctgtttct ccggaccgac atcacgcttg acaccgcgcc
     3601 ggatgagcgc tccaccgagg aactgctcct ggatgcctac gacggcaccg taccagtgcg
     3661 cctcatcgaa accatgttcg atttcggcag gtacctgctc atcagcagct ctcgacccgg
     3721 aggcctgccg gcgaatctgc agggcatctg gaacggtgaa tggaatcctc cctggcaatc
     3781 cgattttcac aacgatgaga acatccagat gaactactgg caggcgttgc ccgggagcct
     3841 gcccgagaca ctggaacctt tcttttccta tttcgagtcc atggtgccgg actaccggga
     3901 gaacgcgcgg aaagtctacg ggtgccgcgg aatccttttg ccaatctgcc agacgaccga
     3961 cgggatgctc tacactgatg tgtggaccaa ctggactgcg ggggcgggct ggatagcgca
     4021 gcatttcttt gactactggc tattcacagg ggacaaggag ttcctcctgc ggcgcgccat
     4081 tccgttcatg aaggaagtgg cgctgttcta cgaggacttc cttttcgagg gcccagacgg
     4141 caggctcgtg ttctcgccct cactctcgcc ggaaaacatc cccgacagac ccggtgcttc
     4201 gcacgcgcag atcaatgcga cgatggacgt ggcggtcgcg agggagctgt tgacgaacct
     4261 gtgttccgcg tgcgaactga tggacgtgga atgcgaaggc gtcgcgcgtt ggcggaagtt
     4321 gctggaaaag ctccctgcgt atgagacgaa caaggatggc gcgattcgtg agtggatcca
     4381 ccccgatctg cccgataact accaccaccg ccacttgtcc cacatctacc cgctgttccc
     4441 cgggcttgag gtgacgaggg agaccgagcc ggagcttttc gaggcgtgcc gcgtggccgt
     4501 ggaaaagcgg ctggtgatcg gcttgtcatc gcagagcggg tggtcgtatg cccacatggc
     4561 gaacatatat gcccggttgg gcaatggaga ccgggcgctc gattgccttg aaaacctgtg
     4621 cagatcggtg gtgggtccaa accttttcac gtaccacaac gactggcggc aacag
//
LOCUS       DAOTNG010000254         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15097_length_4674_cov_4.330808, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000254 DAOTNG010000000
VERSION     DAOTNG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15097_length_4674_cov_4.330808"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="P5179_11250"
     CDS             <1..840
                     /locus_tag="P5179_11250"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRS95837.1"
                     /translation="LEADHLDCYRDFGDIESAFKAYLERLPFFGVAILGIDDPNVRAL
                     VPSIRRRVVTYGFAGDADVRAIDVRSWEKGSRFSVVANGENLGEVALAVPGRHNVQNA
                     LAAIVVARDLGVSVQQIREGLAGFSGVDRRFQVRGKVHGATIIDDFAHHPTAVRVTLD
                     TARSMAGEGARLLVAFQPHLFSRTRDMYEEFGAALASAELVLVLDIYPSRETPIEGVT
                     SRLVAEACSRAGARDVREVHSHEEAAAILRDSARSGDIVLTIGAGDIWKVGTALAETE
                     ENS"
     gene            842..1675
                     /locus_tag="P5179_11255"
     CDS             842..1675
                     /locus_tag="P5179_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRS95838.1"
                     /translation="MAEETPPTTSESGTGLRQRVWAGLVTHKTRVFSRWPWIARLVAF
                     VAYVATGAALWGTGYGIIRLPWLAIDDVVVVGTDQVDVRAIYDAAGIRRGSPLLALDV
                     NAVTAGVKSVPWVRDVRVSRHLPGRVRVEVVERAPVAVFQLDRCYLMDEEGFVTPLNG
                     RTVPDLPIVTGLKAVPFPQRESLEPAGRALAVVAEFPVLRNTVSELSLADSTRMVLIL
                     SPRGTPVSLPRLTGRDRLVMLASVIAYEPDLLMTASHLDARFLGHLAVTSSPSSNGNR
                     M"
     gene            1687..3003
                     /gene="ftsA"
                     /locus_tag="P5179_11260"
     CDS             1687..3003
                     /gene="ftsA"
                     /locus_tag="P5179_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRS95839.1"
                     /translation="MPLGRIVAGLDVGTTKVTTIIAEVDESEAVSVIGVGQTESRGVE
                     RGMIVNLDETVDSIQKSVERAQHMAGCRISSVYVGIGGEHIRGINSHAAVTVGKITNE
                     ISEEDVKRVIDHAQNVSMPADMTVIHVIPQEYRVDDQKHIIQPIGLAGSRLEGEVHIV
                     RGAATAEQNLRRAVQRAGLQVAGIVIQPLASALAVLSPDEQEWGVAMLDIGGGTTDIA
                     VYHRRCIRHTAVLSIGSAYVTSDIAIGCSILMSEAEQLKKQYGRASRQLAEQEHNLPE
                     DPREQPDIRFLRENTRAVLDAKIPEPKFSVELARIIEMRMVELLGAAWFEIRKSGHQE
                     YLASGVVVTGGGAMVKGVAELAQRVFGVPVRIGYPLGVGGIRESVATPANATGVGLVL
                     FGASQLLKTPQTMSMTDAERELERLNQEMQRKPLEGMRRWLKEFFA"
     gene            3107..4549
                     /gene="ftsZ"
                     /locus_tag="P5179_11265"
     CDS             3107..4549
                     /gene="ftsZ"
                     /locus_tag="P5179_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRS95840.1"
                     /translation="MSNDNFELIHEQEPVARIKVIGVGGGGGNAIRRMVDSGLSRVEF
                     IAINTDQQVLHRSGATTQICIGNNVTKGLGAGGRPEIGRQAIDEDRDRVADALGGSDM
                     VFITAGMGGGTGTGASPVVAQIAREIGALTVGVVTTPFAFEQPKRLRRAAEGLEALRE
                     AVDTLIVISNERLLQVLPPRTPINDAFREADKVLLQAVQGITDIILVPGLINLDFADV
                     RTVMSHRGDATMGSGVATGENAASRAAEIAIASPLLEDVSIRGAKGLLVNITSNSSLP
                     QEDVNAAMSVVNREAGADADVFFGVVLDDSVPEGEIRVTVIATGFESRCPSTFTREIM
                     TPAQKPFAVPTSPTVAVSTLETEREPEPEPVAEAVPEPVELPVEEKVERNTVETVVNI
                     PMTENELQIPAWQRKQQEPLQAPAEEPSSLGTAAHVLVPKEPKVAPVSSVPRESLDRE
                     LPRQYGIYAGSTDSADLRMPTFLRKARGRT"
     gene            4599..4672
                     /locus_tag="P5179_11270"
     tRNA            4599..4672
                     /locus_tag="P5179_11270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4633..4635,aa:Met,seq:cat)
BASE COUNT         1003 a         1297 c         1514 g          860 t
ORIGIN      
        1 ctcgaggcgg atcacctaga ctgctatcgc gatttcggcg acatcgagag cgcgttcaaa
       61 gcctatctcg agcgactgcc ctttttcggc gtcgccatac tcggaataga tgatccgaat
      121 gtgcgcgcgc tcgtcccctc catacgcagg agggtggtca cgtacgggtt cgcgggtgat
      181 gcggatgtcc gggcaattga cgtccgttcg tgggagaaag gaagccggtt ctcggtcgtc
      241 gcaaacggtg agaacttggg agaggtagca ctggccgtac ctggtcggca caacgtccag
      301 aatgcgctcg ctgcgatcgt agtcgcgcgg gatctcggcg tttccgtgca acagattcgg
      361 gaaggactgg cgggcttctc tggcgtggac cgtcgcttcc aggtgagggg gaaggtccac
      421 ggggcaacaa tcattgacga cttcgcacac cacccgacgg cagttcgtgt cacgctggac
      481 acggcacgta gcatggcggg agaaggcgcg cgattgctgg tggcgttcca gccccatctg
      541 ttcagccgta ctcgggacat gtacgaggag ttcggcgccg cacttgcgag cgcagaactc
      601 gtgctggtcc tggacatcta cccttcacgc gagaccccta tcgagggggt gaccagccga
      661 ctggtcgcag aggcgtgctc gcgggcgggc gcgagggatg tgcgtgaggt ccattcccac
      721 gaagaagccg ctgccattct ccgcgattct gcaaggtccg gcgacatcgt tctgaccatc
      781 ggcgctggtg acatctggaa ggtgggtacc gctctggctg aaaccgagga gaacagctga
      841 catggcggaa gaaacgcctc cgacaacgtc agaaagcggc accggcctcc gtcagcgagt
      901 ctgggcgggg ctggttaccc acaaaactcg ggtgttctca aggtggccct ggatagcacg
      961 cctggtggca ttcgtcgcgt acgtggccac cggggctgcg ttgtggggta cgggttacgg
     1021 aatcatccgt cttccctggc tcgccattga cgatgttgtc gtcgtgggaa cggaccaggt
     1081 ggacgtgcga gcaatttacg acgcggcggg tatccgacgt ggttcgcctc ttcttgcgct
     1141 cgacgtcaac gccgtcactg cgggcgtcaa atcggttcca tgggtgcggg acgtacgagt
     1201 ctcacgccat ttgcccggca gagtacgcgt cgaggtagtc gagcgcgcac cagtcgcggt
     1261 gttccagctg gatcgctgtt acctcatgga cgaggagggc tttgtaacgc cactgaacgg
     1321 tcgcaccgtg ccggacctgc ctatcgtaac gggattgaaa gccgtaccat tcccgcagcg
     1381 ggaaagcctg gaaccggcag ggcgggcgct cgccgtcgtc gcggagtttc ccgtgctgcg
     1441 aaacacggtt tcagagctct ctctcgcaga ttcaacgcgg atggtgctca ttctctcgcc
     1501 gcgtgggaca cctgtttcgt tgcccaggtt aacgggaaga gacaggctgg taatgcttgc
     1561 atcggtaatt gcgtatgaac cggatcttct catgaccgca tcgcaccttg atgctcgttt
     1621 cctcgggcac ctggccgtca cgtcttctcc gtcgtcgaac ggcaacagga tgtgaggatc
     1681 tgaccgatgc cactggggcg aattgtcgcc ggtcttgatg tcggcaccac gaaagtaacc
     1741 acgatcatcg cagaggtcga tgaatcggaa gcggtgtccg tcattggtgt cggccagaca
     1801 gagtcccgcg gcgtggaacg cggaatgata gtcaatctgg acgaaaccgt ggactcgatc
     1861 cagaagtccg tggaacgagc acaacatatg gccgggtgcc gtatctcctc cgtgtacgtc
     1921 ggaatcggag gagagcacat ccgcggaata aacagccacg cggcggtcac cgtcgggaag
     1981 atcacgaacg agatctcgga agaggatgtc aagagagtca tcgaccatgc gcagaacgtt
     2041 tcgatgcctg cggatatgac cgtcattcat gtgattccgc aggagtaccg cgtggacgat
     2101 caaaagcaca tcattcaacc catcggactg gcgggatcgc gccttgaagg cgaagtccac
     2161 atcgtacggg gcgccgccac tgccgaacag aatctgcggc gcgcggttca gcgcgcaggg
     2221 ctccaggtcg cgggcatagt cattcagcca cttgcgagcg cattggctgt gctatcccct
     2281 gatgaacagg aatggggcgt cgcaatgctg gacatcggtg gcggcacaac agacatcgcg
     2341 gtgtaccacc ggagatgcat acgtcacacg gcggtgttga gcatcggcag cgcgtacgtg
     2401 acgagcgaca tcgcaatcgg ctgttcgatc ctcatgagcg aggcggaaca gctgaaaaag
     2461 caatatggac gggcttcccg gcaactcgcg gagcaggaac acaacctgcc tgaagatccg
     2521 agggaacaac ctgatatacg cttcctccgc gaaaacacgc gggcagtcct cgacgcgaaa
     2581 ataccggaac caaagttttc ggtcgaactt gcgcgaataa tcgagatgcg catggtggag
     2641 ttgctggggg ctgcctggtt cgagatccgc aaatccggcc accaagagta cctggcgagc
     2701 ggggtggtgg tgacgggcgg cggagcgatg gtcaagggtg tagcggaact cgcgcaacgt
     2761 gttttcggcg tccccgtccg aatcggttat ccgctcgggg ttggcggaat acgagaaagc
     2821 gtcgcgacgc cggcaaacgc gacgggagtc ggactggttc tatttggagc cagccagttg
     2881 ttgaaaacgc cacaaacgat gtccatgaca gacgctgaga gagagcttga gagactcaac
     2941 caggagatgc agagaaagcc gcttgagggc atgcgtcgtt ggctgaagga attcttcgca
     3001 tagaaagtag actcgtcgga aacgtcccaa aacaactgcg atgaagccgg agtggtcggg
     3061 cacggaaggg ttgccgcaag ggtttcggag cagaggaggt ggagaaatgt caaatgacaa
     3121 cttcgaactg atccacgagc aggaacccgt ggcaaggatc aaggtcatcg gtgtcggggg
     3181 tggtggcggc aacgccattc gaaggatggt cgacagtggc ctttcgcgcg ttgaattcat
     3241 cgccatcaac acggaccagc aggtgctcca ccgctcgggc gcaaccacgc agatctgtat
     3301 cggcaacaac gtaaccaaag ggctcggcgc ggggggccgt ccagaaatcg gcagacaggc
     3361 gatagacgag gacagagatc gcgttgccga cgcattgggt ggctctgaca tggtcttcat
     3421 caccgccggg atgggcggcg gaaccggcac gggagcatcg ccggtagttg cccagatagc
     3481 gcgcgaaata ggtgcgctca cggtaggggt ggtaactact cctttcgcat ttgagcagcc
     3541 gaagcgcctc cgcagagcgg cagaaggcct cgaagcactt cgggaggcgg tcgacacact
     3601 tatcgtgatt tccaatgagc gcctgctgca ggttctaccg cccaggacac cgatcaacga
     3661 tgcgttccgg gaggcagaca aagtgcttct ccaggccgtt caaggaatca ccgacataat
     3721 cctggttccg gggttgatca atctcgattt cgctgacgtg cggacggtga tgagccatcg
     3781 aggcgacgcc accatgggaa gcggcgtggc aaccggagaa aatgccgcaa gcagggccgc
     3841 ggagatcgct atcgcaagcc ccttgctcga ggacgtctcg atcagaggcg caaaaggact
     3901 cctggtgaat atcacctcca attcgtcgtt gccgcaggaa gacgtaaacg cggcgatgtc
     3961 agtggtgaac cgggaagctg gagccgatgc ggacgtgttc ttcggggtgg ttctcgacga
     4021 cagcgttccg gaaggcgaaa ttcgggtgac agtcatcgca accgggttcg agagccgttg
     4081 cccgagcacg ttcaccaggg agatcatgac tcccgcgcag aagcctttcg cggtaccgac
     4141 ctctccgacg gttgcggtca gcacactgga aacggaaagg gagccggagc cagagccggt
     4201 tgcggaagcg gtgccggaac cggttgaatt gcccgttgag gagaaagtcg agcgcaatac
     4261 ggtcgaaacg gtcgtcaaca tcccgatgac agagaacgag cttcagatac ccgcctggca
     4321 gcggaaacaa caagagcctc tccaggcccc ggcggaagaa ccctcatctc tgggaacagc
     4381 agcgcacgtc ctggttccca aggaaccgaa ggtagctccc gtttcatcgg tcccgcggga
     4441 atcactggac cgcgagctcc cacggcaata cggtatttac gccggaagca cggattctgc
     4501 ggacctccgg atgccgacat ttctccggaa ggcacgcgga cgaacgtaac ggagcttgcc
     4561 cggatggcgg attctgcgta gattagttat ctgttttcgg cggcgtagct cagctggtta
     4621 gagcagcgga atcataatcc gcgtgtccgg ggttcaagtc cctgcgccgc tact
//
LOCUS       DAOTNG010000255         4660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15138_length_4660_cov_4.347231, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000255 DAOTNG010000000
VERSION     DAOTNG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15138_length_4660_cov_4.347231"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2813
                     /locus_tag="P5179_11275"
     CDS             <1..2813
                     /locus_tag="P5179_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS95841.1"
                     /translation="LDNAGLAVLNRGTIAASAEPGGELFLALQHAAGWCDWATPHYLG
                     FPFVPERKSTVHHYAILPFAGSWSEARIPEEAQAYNRPFTPAQTGIHEGELPSKGQFV
                     NLQGESLAVSAVKPAGQPSAQFLRRNASAADGLVMRVWNWRGVYCPAGLSLEAGLESA
                     AMVDLAERNPHPIPVRDGAVKFSVGPNAIETLLLTPARFKEQTSLSTQGTATSRLVES
                     RWWRQNLGAAPRGNTPVSVGLRGDLLPGERGTVVLSIAHNRLGESFSGTVRLYGPSGW
                     TISPTLVPVTLGPRSSTAFDIAVAVPQDMPGRLVCELAYEGNLYVDSLLCGNAAQPEV
                     QADQRGDGFVVRVSNPSRDTLPISVDLITPLDTWAELDAFALSPIPHQTAHFTLAPGE
                     QREAVFAAGAHLIGDFWAFAKVVARESCQYLRLPVRRNATLHAEAGTSFCLNAPLSDC
                     ALLGTVSSNSKLAFSVVARQQPAAGIAPSPIPMAGYWSLSPVEGAEHVQAPRLRFRIN
                     PEHGRKLHDAATLSMATRRGSAWVPVPTTFDRSRWELLAEVSPETLRERTDWTIFAPV
                     NELWRVSVGRRFFEAHPEVADIDGDGEPEVVIGTAGNGLCALSSRGRILWRRKFGGWM
                     WQSPNTTLADVDGNGNLEIVVAPSDRSLILLDAAGHVIWRLDDLPFAVKQKPLVADIN
                     GDGRNEILVSIDDNGVFAFDCNGKRLWNCAMEPVRLSAPVMAGGTAGAPEILVNAGGR
                     ALAVLNANGALVRMTRFSAGWARDSYVADPVSTDLLTPGTPNILFAGHDGFLRLLAAD
                     GTQVWECDLGSPVARAPVVLPAEGGAPPVIVCGAARQTLCGITPVGSIAWRTTPRAHV
                     QSPLGCADVDGDGHLEIILGCGRDQRTVIFDREGNEKASIPYSAIWTNTAVPLGNSSP
                     GRASVLVTGDWNGDIVAATPHI"
     gene            2977..3780
                     /locus_tag="P5179_11280"
     CDS             2977..3780
                     /locus_tag="P5179_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRS95842.1"
                     /translation="MKLGCSTILYGVHSTEDALSGIKKAGYDGIELCAIPGMGFHIEP
                     NKTEKEYRELKKKIDDHGLIVESIGGSGNDPYDPDPDSKFRSLMRAAAIIGAPAITTG
                     SGGKSDNQEDMKRAAEVFNKLAEFGKTVGTKVSIKPHVGGVVYNTPSSLEFMSLVDTK
                     WIGLNVDASHLWRAPKWEAGEESIPKLAKYIFTARIRDTLNHDQPIGPVETQVPGGGA
                     MNLKAIMDAFKKVPGLDIVTVEIVGTAQWELSAIQDVVERTYAALKKLV"
     gene            3892..>4660
                     /locus_tag="P5179_11285"
     CDS             3892..>4660
                     /locus_tag="P5179_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95843.1"
                     /translation="MVRTVVLGLDEVGREYARASKRDSASSLVGVYDPDRARADRLGK
                     ELGIPVFDRPEELLEVAKPQVCVLSALEGKETLASDLLSRGIHVLSTLPVAPDAEQCE
                     KLAVRGSEKDGILAADFHLRFTPAMDKAREWVSGGEIGSPLFINMNLWRDVPDRHGDN
                     PYHLFVSLGAHGVDVIRQFVGGVERVQCFGVRDKQGIWTSAQINMMCSNGAVGHLTIS
                     YEMVTHHPIARCEIAGSRARVVIENVYEDTTLYVHERP"
BASE COUNT          933 a         1427 c         1430 g          870 t
ORIGIN      
        1 ctctggacaa cgccgggctg gcggtgctga accggggcac gattgcagcg agcgcggaac
       61 cgggaggcga attgttcctt gcgctgcagc acgcggccgg gtggtgcgac tgggcaacgc
      121 cgcactacct cggctttccc ttcgtcccgg aacgaaagag cactgtacac cattacgcaa
      181 tcctcccgtt tgcgggctcg tggagcgagg cacgaattcc ggaagaggca caggcgtaca
      241 accggccttt cacaccagcg cagaccggta tccatgaggg ggagcttcct tcaaaaggcc
      301 agttcgtcaa tcttcaaggc gaatcacttg cagtctccgc cgtcaaacct gcaggtcaac
      361 ccagcgcgca gttcctgcga aggaacgcga gcgcagcgga cgggctggtg atgcgcgtct
      421 ggaactggcg cggggtatat tgccccgcag gactctccct ggaagccggc ctcgaatcgg
      481 ctgccatggt cgacctcgca gaacggaatc cccatccgat tcccgtacgc gacggtgcgg
      541 tcaagttctc tgtcggcccc aacgcgatag aaactctcct gctcacgccc gcgcgattca
      601 aggagcagac ttcgctgagc acccagggga cggcgacatc gcgactcgtg gagtcccggt
      661 ggtggcggca gaacctcggg gccgcacccc gtggaaacac gcccgtatcc gtgggccttc
      721 ggggtgatct tctccccggt gaacgcggca ccgttgtgct gtccatcgct cacaatcggc
      781 tcggggaatc cttctccggc acggtccggc tttacgggcc ttccggttgg actataagcc
      841 cgaccctggt gcccgtcaca ctgggcccga ggtccagtac tgcttttgac atcgccgtgg
      901 ccgtaccgca ggacatgccg gggcgcctcg tgtgtgaact ggcgtacgaa ggcaacctgt
      961 acgtcgacag ccttctctgt gggaacgccg cgcaaccaga ggtgcaggcc gatcaacggg
     1021 gcgacgggtt cgtagtgcgc gtgtcaaacc cctctcgcga cactctgccc atttcggtgg
     1081 atctcattac cccgctggac acatgggcgg aactggacgc gtttgccctg agccccattc
     1141 ctcaccagac cgcgcatttc acccttgcgc ccggggaaca gcgggaggcg gtgtttgccg
     1201 ccggcgcgca ccttatcggc gatttctggg catttgcgaa ggttgttgca agggaatcat
     1261 gccagtacct ccgtctgccg gtccgcagaa acgccaccct gcacgctgag gcaggtacca
     1321 gtttctgtct caatgctccc ctttcggact gcgcactcct cggtaccgtt tcctccaaca
     1381 gcaaactggc tttcagtgtg gtggcccggc aacaaccggc tgctggaatt gctccttccc
     1441 cgatcccaat ggccggatac tggagccttt ctcccgtgga aggcgccgaa cacgtccagg
     1501 cgccgcgttt gcggttccga attaacccgg aacacggacg gaagctccat gacgcggcga
     1561 cgctttcgat ggccacacgg cgaggcagtg catgggttcc cgtgccgacg acattcgacc
     1621 gttcccggtg ggaactgctt gccgaagtgt cgccggaaac actcagagaa cgtaccgact
     1681 ggacgatttt cgcgcctgtc aacgaactct ggcgcgtatc cgtgggacgc cggttctttg
     1741 aggcgcaccc cgaggtcgcc gacatagacg gagacgggga gccggaggtc gtcattggca
     1801 cggcgggaaa cggcctgtgc gctctttcct cccggggccg gatcctgtgg cgccggaagt
     1861 tcggtggatg gatgtggcag tcccccaaca ccactctcgc agacgttgac gggaacggca
     1921 accttgaaat cgtggtcgcg ccctccgacc gctctcttat ccttcttgac gctgccgggc
     1981 acgtcatctg gcggctcgac gatctgccgt tcgcggtgaa gcagaaaccg cttgtcgcag
     2041 atatcaacgg ggatggcagg aacgaaatcc tcgtgtcgat cgatgataac ggcgtgttcg
     2101 cgttcgactg caacggaaaa cgtctctgga actgcgcaat ggagccggtg agactgtcgg
     2161 cccccgtcat ggccggcggt acggccggcg cgcccgagat tctcgtcaac gcgggaggca
     2221 gagccctggc ggtactgaac gccaacggtg cgctcgtccg gatgacgcgg ttctcggccg
     2281 ggtgggcgcg cgactcgtat gttgccgatc cagtctctac cgatctgctc acaccgggta
     2341 cgccgaacat cctgtttgcc ggacatgatg gattccttcg ccttctggca gcggatggaa
     2401 cgcaggtctg ggagtgcgac ctcggttctc ctgtggcgcg tgcgccggtg gttctccccg
     2461 cggagggcgg cgcacctccc gttatcgtct gcggggcggc gcggcagact ctgtgcggta
     2521 tcacacccgt cggaagcatc gcatggcgga caacgccccg ggctcatgtc caatcgcccc
     2581 ttgggtgcgc ggatgttgac ggggacgggc acctcgagat catcctcggg tgcgggcggg
     2641 atcagaggac cgtaatcttc gacagagagg gaaacgagaa ggcatcaatc ccgtactctg
     2701 ccatatggac gaacacggca gtaccgctcg gcaactcctc accgggaagg gcgtctgtcc
     2761 tcgtgaccgg ggattggaac ggcgatatcg tcgcggccac cccgcacatt tgaggacctt
     2821 gcggggactg gtctgttgcg cgatggacgg aacgcagcgc gaccaggcgg gcgctgcggc
     2881 ccagcggatt gatccccgga tcggtctacc ctattctctt tagacaaagg gcacgggcaa
     2941 catatccctt ctccatcaca catcggaggt tctggcatga aactcggttg cagtacgatt
     3001 ctctacggcg tccacagcac agaggacgct ctcagcggca tcaagaaagc gggctacgac
     3061 ggaattgagc tttgcgcgat tcccggaatg ggctttcata tcgagcccaa caaaaccgag
     3121 aaggaatatc gggaactcaa aaagaaaatc gacgatcacg gccttattgt ggaatcaata
     3181 gggggaagcg gcaacgaccc gtacgatccc gatcccgact ccaaattccg gtctctcatg
     3241 cgagcagccg caataatcgg cgcgccggca attaccacgg gatctggcgg aaagagcgat
     3301 aatcaggaag acatgaagcg ggccgccgaa gtcttcaaca agcttgcgga gttcgggaaa
     3361 acggtcggca ccaaagtgag catcaaaccg cacgtcggag gagtcgttta caacacgcct
     3421 tcgagcctcg aattcatgtc gctcgtagac acgaaatgga tcggcctcaa cgtcgacgcc
     3481 tcccatctct ggcgcgcacc gaaatgggaa gccggtgagg agtccatccc aaaactcgcg
     3541 aagtacatat tcactgcccg gattcgtgat accctgaatc atgaccagcc aatcggcccg
     3601 gtggaaaccc aggttcccgg tggcggcgcc atgaacctga aagcaatcat ggacgcgttc
     3661 aaaaaggtcc ccgggttgga tattgtcact gtggaaatcg taggaacagc gcaatgggag
     3721 ctgagcgcga tacaggacgt tgttgaacgc acgtacgcgg cgctcaaaaa gctcgtgtaa
     3781 atgcgaatga agggcgatgg gagggcagcc ggaacgctgc tcctcccgtc tctgtggtgc
     3841 ctcatcccgt ggcttctccg attgcttctc catcctgaaa aggcgcattg aatggtaaga
     3901 accgtggttc tcggcctcga cgaagttggc cgcgaatacg cccgcgcatc aaaacgggat
     3961 tccgcctcaa gccttgtcgg cgtgtacgac ccggaccgcg cgcgggcaga cagactaggc
     4021 aaggaactgg gtatcccggt attcgaccgc cctgaagagc tgctcgaagt cgcgaaaccc
     4081 caggtttgcg tcctgtccgc attagaggga aaagagacgc tcgccagcga tctcctctca
     4141 cggggaattc acgtgttgtc cacactcccg gtggctcccg acgcggaaca atgtgagaag
     4201 ctcgcggttc gaggcagcga aaaagacggc attcttgcag ccgactttca tctgcgcttc
     4261 acaccggcca tggacaaggc gcgcgagtgg gtgtccgggg gagagatcgg ctctccactg
     4321 tttatcaaca tgaatctctg gcgagatgtc ccggacagac acggcgacaa cccataccat
     4381 ctgttcgtct cgctcggggc ccacggtgtg gacgtcatcc gccagttcgt cggaggcgtc
     4441 gagcgggtgc agtgcttcgg cgtccgcgac aaacagggga tttggacctc ggcccagatc
     4501 aatatgatgt gctccaacgg tgcagtaggg catctgacga tcagttacga gatggtgaca
     4561 caccatccca tcgctcgttg cgaaatcgcc gggtccagag cgcgcgtggt gatcgagaac
     4621 gtgtacgagg atactacgct ctacgtccac gaacggcccg
//
LOCUS       DAOTNG010000256         4648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15193_length_4648_cov_5.377313, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000256 DAOTNG010000000
VERSION     DAOTNG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15193_length_4648_cov_5.377313"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="P5179_11290"
     CDS             complement(<1..947)
                     /locus_tag="P5179_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95844.1"
                     /translation="MKRLNNLVVELASHAKLESGDVVKFRTPREGWVFISCEGKKPET
                     RQLRINGRPHPWKSSLAGNLEAMCYLPQGEHVLAVHGKPARRLAIRSIPELLFTKYGY
                     NPFVKDYGPYDWQFLKRYVLPHVNTVIGTGSPEQDDQNREWRSAGGKWIVERGISFDK
                     SARPDAGEVVSSWGGEPGMADPAFDGLLLDEFFSGDDARYPAFAEALRRLGADPKFKG
                     KKLYPYCGSHYPDRNDWGNYNAEDTSNSSVPFWRAAIQSGSRIAWERYLQERHDKEIA
                     KDLIEERIGEKIRLWQKVFPSVQESMIIAFGYMSMGFAL"
     gene            complement(1003..4206)
                     /gene="carB"
                     /locus_tag="P5179_11295"
     CDS             complement(1003..4206)
                     /gene="carB"
                     /locus_tag="P5179_11295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRS95845.1"
                     /translation="MPRRDDIKKVMIIGSGPIIIGQACEFDYSGTQACKALRRLGYQI
                     VLANSNPATIMTDPGMADFTYIEPLNKDTMEEIIRKERPDALLPNLGGQSGLNLSSEL
                     AAAGVLDKYGVKVIGVNVDAIKRGEDRLAFKQTMESLGIDMPKSEIANSVEEAEEIAQ
                     RLDYPLVVRPAYTMGGTGGGFVYNLEELRTIVARGLAASLVGQVLIEQSVLGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSFCTAPMLTISPELQLKLQDYSYRIVEAIE
                     VIGGTNIQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAYVSSLLAGGLTLDEI
                     PYWREGTLEKYTPGGDYVVVKFARWAFEKFKGVEDRLGTQMRAVGEVMSIGKTYKEAF
                     QKAIRSLETGRWGLGFAKDFNSRSLEELMEMLRWATSERQFIMYEALRKGASVAELHE
                     RTHIKHWFIEQMKELVLLEEELLRFKGSLPPDDLLIRAKKDGFADRYLAKILKVPESD
                     IRRRRTELGVVEAWDAVRVSGATDAAYYYSTYNGEDRVPVTQNKKKIMVLGGGPNRIG
                     QGIEFDYCCVHAAFALRDAGYESVMVNCNPETVSTDYDTSDKLYFEPLTLEDVLAIYE
                     KEKPEGVIVQFGGQTPLNLAAELAANGVRILGTSPESIDLAEDRDRFRMRMRELGIPQ
                     PDSGMAGSVEEAVRVAAEIGYPLIVRPSYVLGGRAMEVVHDEEMLREYVAKAVDISPE
                     RPILIEQFLQNAIEAEADAIADGTDAFVPAVMEHIEFAGVHSGDSACVIPPVSIPQKH
                     IDTIEEYTRRIAVEFKVVGLMNVQYAIFDDTVYILEANPRASRTVPIVSKVCAISMAN
                     LATRLMLGAKLPDLNLRRRPIPHFGVKEAVLPFNMFPEVDPILGPEMRSTGEVLGIAE
                     NAGLAFFKAEAAATPPLPLHGTVLITVTDPDKPGALEAARRFVQLGFSIRATEGTHRF
                     LTENGVPSTPILKMREGRPNIADAIKNREIHLVVNTPGGKRSLTDDSYIRKAAIKYKV
                     PYVTTTAAAIAAAKGIAAWRQGRGEVKSLQAYHADIG"
     gene            complement(4414..>4648)
                     /locus_tag="P5179_11300"
     CDS             complement(4414..>4648)
                     /locus_tag="P5179_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95846.1"
                     /translation="EHGELVAIETVTRREVGRVAVGSQAIGIVLSPEGDHAFVGCEHK
                     DGVHVVDIRKLAVVERFFTGNGSDAMAVWYPPR"
BASE COUNT          908 a         1411 c         1267 g         1062 t
ORIGIN      
        1 ttcaacgcga accccatcga catatacccg aacgcgataa tcatggactc ctgaacgctc
       61 gggaaaacct tctgccacag acggatcttt tcgccgatcc gctcttcgat gagatccttc
      121 gcgatctcct tgtcgtgacg ttcttgcagg taccgttccc acgcgatgcg cgacccgctc
      181 tgtatcgccg cccgccagaa cgggacgctg gaattgctcg tgtcctcggc attgtaatta
      241 ccccaatcat tccggtcggg gtagtgggac ccacagtaag ggtacagctt tttccctttg
      301 aacttcgggt ctgcccccag ccggcgtagg gcctccgcaa aagccgggta acgggcatcg
      361 tccccgctga agaactcgtc cagcagcaga ccgtcaaaag ccgggtccgc catcccgggt
      421 tccccgcccc acgaagaaac aacctcaccg gcgtcgggac gtgcgctctt gtcgaagctt
      481 atccctcgtt cgacgatcca tttcccgcca gcgctccgcc attccctgtt ctggtcatct
      541 tgctcggggc tgcccgtgcc tatcacggtg ttgacgtgag gaagcacgta tctcttcagg
      601 aactgccaat cgtagggccc ataatccttg acgaacggat tgtatccgta cttcgtgaac
      661 agcagttctg ggatcgatct gatcgcaagg cgtcgagcag gtttcccgtg gacggccaat
      721 acgtgctcac cctgggggag gtagcacatg gcttccaggt tgcccgccaa ggagcttttc
      781 cacgggtgag ggcgtccgtt gattctcagt tgccttgttt ccggcttttt cccctcgcag
      841 gatatgaaga cccagccctc gcgaggggtg cggaacttca ccacgtcgcc ggattcgagt
      901 ttcgcgtgcg acgcgagctc gaccactagg ttgttcaacc ttttcattcc gacctcccag
      961 cgagtgcccg gctctactca gaatccgttt cagagcccgg gatcatccga tatccgcgtg
     1021 gtaggcctgc aggctcttta cttccccacg gccctgcctc catgcggcaa ttccttttgc
     1081 cgcggcgatc gcggcagcag tcgtagtcac gtaaggaact ttgtacttta tcgctgcttt
     1141 gcggatgtaa gaatcgtccg taaggctcct tttgcccccg ggcgtgttca ctacgaggtg
     1201 tatttcgcgg ttctttatcg catcggcaat attcgggcgt ccctcgcgca tcttgaggat
     1261 gggagtagac ggtaccccgt tctccgtcaa gaaacggtgc gtgccttcgg tagcgcgtat
     1321 ggagaacccc agttggacga atctccgggc cgcttccaac gcccctggct tgtccggatc
     1381 ggtgacggtt atcagcaccg taccgtggag cggaagaggc ggcgtagcag cggcttcagc
     1441 tttgaaaaag gccagtcccg cgttttcggc aattccgaga acctctccgg tggatctcat
     1501 ttccggtcca aggatcgggt ccacctccgg aaacatgttg aacggaagta ccgcttcctt
     1561 gacaccgaag tgcgggatcg ggcgcctgcg caggttgaga tcagggagct tcgctcccag
     1621 catcagccgc gtggcgaggt tcgccatgga gatggcgcag accttgctca cgatcggaac
     1681 ggttctgctg gcacgcgggt tcgcctcgag gatgtatacc gtgtcgtcaa aaatggcata
     1741 ctggacattc atcaggccaa ccactttgaa ctcaactgca atccgtctgg tgtattcctc
     1801 aatcgtgtca atgtgtttct gggggatgct gacgggcggg atgacgcacg cggaatcgcc
     1861 ggagtgcacg cccgcaaatt caatgtgctc cataactgcc gggacaaatg cgtccgttcc
     1921 atctgcgatt gcatctgctt ccgcctcgat cgcgttttgc aggaactgtt ctatgagaat
     1981 cgggcgctcc ggggagatat ctacggcttt cgcaacgtat tcacgcagca tctcctcgtc
     2041 gtgcaccact tccatcgcgc ggccgccgag aacgtaggac gggcgcacga tcagcgggta
     2101 cccgatttca gcggcaacac gaacggcctc ctcgacggaa ccggccattc ctgaatcagg
     2161 ctgcggtatg cccagctccc gcatgcgcat gcggaaccga tcgcggtctt cggcaaggtc
     2221 gatggattct ggcgacgttc ccaggatgcg cacgccattc gcggccagtt cggcggcaag
     2281 gttgagtggc gtctgcccgc cgaactgaac gattacgccc tccggcttct ccttttcgta
     2341 aatcgcaaga acatcctcga gtgtcagcgg ctcgaaatag agcttgtcgg atgtgtcgta
     2401 atccgtagag acggtttcgg gattgcaatt caccatgacg gactcgtacc cggcgtcccg
     2461 caaggcaaag gcggcgtgga cacagcagta atcgaattct ataccctgtc cgatcctgtt
     2521 tggcccacca ccgagcacca tgatcttctt cttgttctgt gttaccggta cgcgatcttc
     2581 gccattgtac gtggaatagt aatacgccgc atccgtcgct ccgctcacgc gtacggcatc
     2641 ccacgcttcc acaacgccga gttcggttct tcttctccgg atgtcgctct ccggaacttt
     2701 gaggatcttg gcgaggtacc ggtcggcgaa accatccttt tttgcacgga tgagcaaatc
     2761 gtccggcggc aacgaccctt tgaacctgag cagttcctct tccaggagaa cgagctcctt
     2821 catctgttcg atgaaccagt gcttgatgtg cgtgcgctcg tggagctcgg caaccgaggc
     2881 tccctttcga agtgcctcgt acatgatgaa ctgcctctcg ctggttgccc agcggagcat
     2941 ttccatcagt tcttcgaggg aacggctgtt gaagtccttc gcgaagccca ggccccaacg
     3001 gcctgtttcc agagaccgga tagccttttg gaaagcttct ttgtaagtct tcccaatgct
     3061 catcacttca ccgacagcac gcatctgcgt gccaagccgg tcctcgacgc ccttgaattt
     3121 ttcaaacgcc caccgcgcga acttgacgac aacgtagtct ccacccggcg tgtacttctc
     3181 gagtgtccct tcccgccagt aggggatttc atcaagcgtg agaccgcctg cgaggagcga
     3241 agagacgtac gcgatcggga aaccggtagc tttcgaggcg agagccgacg aacgggaggt
     3301 ccggggatta atctctatca cgacgacgcg acctgttttc gggtcatgcg cgaactggat
     3361 gtttgtcccg ccgataacct cgattgcctc gactattcga taggagtaat cctgcaattt
     3421 gagctggagt tcgggggata tggtgagcat cggggcagtg cagaatgaat ctccggtgtg
     3481 cacgcccatc gcgtccacgt tttcaatgaa gcacaccgta atcatctggt tcttggcgtc
     3541 gcggacgact tccagctcca gttcctccca cccgagtacc gattgctcta tcagcacctg
     3601 cccgacgagg cttgcggcca gaccacgggc gacgatagtc ctgagctcct cgaggttgta
     3661 gacaaaacct cctccggtcc ctcccatggt atacgcgggc ctcaccacca gagggtaatc
     3721 aaggcgttgc gcgatctcct cggcttcttc gacgctgttg gcgatctcac ttttcggcat
     3781 gtcgatgccg aggctttcca tggtttgttt gaacgcgaga cgatcctccc cgcgtttgat
     3841 cgcatcgaca ttcaccccga tgaccttcac gccgtatttg tcgagaacac ccgctgctgc
     3901 aagttcagat gacaggttca ggcccgattg tcctccgaga ttcggcagga gagcgtctgg
     3961 ccgttccttg cgtatgatct cctccatcgt gtctttgttc agaggttcga tgtaggtgaa
     4021 atcggccatt cccggatccg tcatgatggt tgccggattc gagtttgcaa ggacgatctg
     4081 gtatccaagc ctcctgagag ccttgcacgc ctgtgttccc gaataatcaa actcacaagc
     4141 ctgcccgatg atgatgggac cagaaccgat gatcatgacc tttttgatgt cgtcgcgtcg
     4201 gggcatacct ggcagctcct cagggtttct gtgcattccc ggcaccttga aaaaccccga
     4261 acgtgctgcg catgctcagg ggtcaggcgg caggatcgca tggtgcgaag aacgtgcaaa
     4321 agcgggctga gcgtgggaac tcaacagaat gaaaaagtga tcactcaaat ttccggaaaa
     4381 gacgggaacg cgtcaactga gtcattcggg gcttcagcgc ggggggtacc acacggccat
     4441 tgcgtcggac ccattccccg tgaaaaagcg ctcaactact gcaagcttac gaatatccac
     4501 cacgtgcacg ccgtccttgt gctcgcagcc gacgaacgca tgatcacctt cgggtgagag
     4561 aacgattccg atcgcctgac ttcccacggc aacgcgccca acctctctgc gtgtcaccgt
     4621 ttcaattgca accagttccc catgctcg
//
LOCUS       DAOTNG010000257         4629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15247_length_4629_cov_4.577394, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000257 DAOTNG010000000
VERSION     DAOTNG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15247_length_4629_cov_4.577394"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="P5179_11305"
     CDS             complement(<1..811)
                     /locus_tag="P5179_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS95847.1"
                     /translation="MRSVAFEIGGKALLDGVSVTISPGEYVAVMGRNGSGKTTLIRHF
                     NGLLLPTSGECFAFGLPTRDPRCLREIRRRIGLVFQDPDDQMVAVTVEDEIAFGLENR
                     AVPPEEMDQRVEAVLDIFHLQHVRKRSTSALSGGEQQRVAIAAVWATNPDVLVLDEPT
                     SMLDLVSSVALLELLNEIRGSKAVVHVTQNLQEAARAERLIVMDEGRIVFDGPPQQAL
                     EESDSLRRWGIAAAGRLASRNTPPGTSIAISTEALNHVRDDGNETNHVLKDV"
     gene            876..1169
                     /locus_tag="P5179_11310"
     CDS             876..1169
                     /locus_tag="P5179_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRS95848.1"
                     /translation="MIGPTEPNGGQNSVEVNLGEHVEGRYSNVVMITHTATEVILDFS
                     VHVPGLPGPKVVSRIIITPEHAKRLLSALDDNIKKYEKRFGTIHHPEERNPPA"
     gene            complement(1170..1559)
                     /locus_tag="P5179_11315"
     CDS             complement(1170..1559)
                     /locus_tag="P5179_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRS95849.1"
                     /translation="MSETPQSEPKSVLRRYRKSLLSVWALYSAALTVSMMVPGDSVPD
                     LGFFGWDKLAHLVVFIVLAFLTAGAFLTRDCVITIVMSWGVLLGCATEFAQALAPGRI
                     PSVGDAAANLIGTTIGALLVKRYLLRD"
     gene            complement(1552..2601)
                     /gene="purM"
                     /locus_tag="P5179_11320"
     CDS             complement(1552..2601)
                     /gene="purM"
                     /locus_tag="P5179_11320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRS95850.1"
                     /translation="MSPAEPLRTVTYADAGVDIEKANRTKELLKELVRKSYTPQVLTE
                     LGSFGGLFEADFGDVEDPVLVASTDGVGTKLKVAVVAGRHTTVGMDLVNHCVNDILVM
                     GARPLFFMDYIALGRHDTQIVVDVVEGLSAACTAVGCALLGGETAEMPDMYRPGEYDL
                     AGTIVGVVGKREVITGANVRHGDVLIGLMSDGLHTNGYSLARKLIFDIAGWNTDTFVP
                     EWGRTVADALLAPHRCYLHALLPLIARGMIHAMAHVTGGGITDNVPRILPEGLSARVR
                     TAAWDTPPIFRTLVRLGNLNAEEAFRAFNMGIGMVLVVDSTSADTVLGELTTAGETAW
                     LIGEVVSGDRNVQYV"
     gene            complement(2614..3357)
                     /locus_tag="P5179_11325"
                     /pseudo
     CDS             complement(2614..3357)
                     /locus_tag="P5179_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(3395..4372)
                     /locus_tag="P5179_11330"
     CDS             complement(3395..4372)
                     /locus_tag="P5179_11330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRS95851.1"
                     /translation="MPTWNVFVTRRIPDAGLDLLRNAGVNFEVNPEDRVLTHEELLAG
                     VSGRDGVLCLLTDIVDESVLEAARGARVFANYAVGYNNVDVAAATKRGIMVTNTPGVL
                     TETTADMAWALIMATARRIVEGDRLVRAGEFHGWGPMMLLGMDVFGSTLGIVGAGRIG
                     CAVARRAQGFGMKVLYTDSAPNPEISSLNAIRVDLDELLKASDIVTLHVPLLEETRHL
                     IGKREFALMKNGAILVNTSRGPVVDERALVEALKSGSIAGAGLDVYEDEPQLAPGLAD
                     CKNAVLTPHTASATIATRNKMAVMAATNLLAALRGDTPPNLVNPEVLTG"
     gene            complement(4395..>4629)
                     /locus_tag="P5179_11335"
     CDS             complement(4395..>4629)
                     /locus_tag="P5179_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRS95852.1"
                     /translation="SLPEIDLLVEIASSTPGCYGARLTGAGFGGAVISLVDTGSADVF
                     AEEVVEEYRKKTCKAGTAMRVVPDEGSRKEEPV"
BASE COUNT          977 a         1443 c         1265 g          944 t
ORIGIN      
        1 tcacgtcttt caagacgtgg ttggtttcat tcccatcatc gcgcacgtga ttgagcgctt
       61 cggtggaaat ggcgattgat gtccctggtg gtgtgttccg cgacgctagc ctgcccgcgg
      121 cggcgattcc ccacctgcgc agggaatcac tttcctccaa cgcctgctgc ggcgggccgt
      181 cgaacacgat gcgtccctca tccatcacga tcaaacgctc agcacgtgcg gcttcctgaa
      241 ggttctgtgt tacgtggacg accgctttgg agccccggat ttcgttaagg agctccaaca
      301 gtgccacaga ggaaacgaga tcgagcatgg aggtgggctc gtcgagcaca agaacgtcgg
      361 ggtttgtcgc ccagaccgct gcaatcgcga cacgttgctg ttcaccgccc gaaagcgccg
      421 aggttgagcg tttcctgacg tgctggaggt ggaagatgtc caataccgcc tcgactcgtt
      481 ggtccatttc ctcaggggga acggcacggt tctccaagcc gaatgcaatc tcgtcttcaa
      541 ccgtcacagc caccatctga tcgtcagggt cctgaaaaac cagaccgatt cgccgcctga
      601 tttcgcgcag gcaccgcggg tcccgggtcg gcagcccgaa tgcgaagcac tcacctgatg
      661 tgggaaggag caggccattg aaatgccgaa taagtgttgt ctttcccgag ccgttacgcc
      721 ccataactgc gacatactca cccggcgaaa tcgtgactga cacaccgtca agaagagctt
      781 tcccgccgat ctcgaacgct accgagcgca tttcaatcgc atgcggtgcc ggagcaactt
      841 gtgtggtggc tgaggaaacg gtatcattca tcactatgat aggacctaca gaacccaacg
      901 gcgggcaaaa ctcggtggaa gtcaatctcg gggagcacgt cgaaggacgg tactcgaacg
      961 tcgtcatgat tacgcacacg gcgaccgagg taatcctcga tttctcggtt catgtgccgg
     1021 gtctgcccgg gccgaaggtc gtgtcgagga taatcatcac gcccgagcat gcaaagcgcc
     1081 ttttgagcgc ccttgacgac aacatcaaga aatacgagaa gcgcttcgga acgattcatc
     1141 accctgagga acgaaacccg cccgcctgat caatccctca agagataccg tttcaccagc
     1201 agcgcgccaa tagttgttcc gatcagattc gccgccgcat ctccgactga cggaatacgt
     1261 cccggcgcaa gcgcctgcgc gaactcggtt gcgcacccca gcaacacccc ccagctcatc
     1321 acgatcgtga tgacgcagtc ccttgtaaga aatgccccgg cggtcaggaa ggcaagaacg
     1381 ataaaaacaa ccaggtgcgc aagcttgtcc cacccgaaaa atccgaggtc tggtacgctg
     1441 tcgccgggca ccatcatgct gacggtcaat gcggccgagt acagcgccca gacggacaga
     1501 agcgactttc gatatcggcg gagaactgac ttcggttcgc tctgaggtgt ctcagacata
     1561 ctgaacgttc cgatcccccg agactacttc gcctatcaac cacgccgttt ccccggctgt
     1621 ggttagttct ccgagtacag tatccgccga ggtgctgtcg accacgagga ccataccgat
     1681 acccatattg aaagcccgga acgcttcttc cgcgttgaga ttcccgagcc tgaccagagt
     1741 ccggaaaatc ggtggtgtgt cccacgctgc cgtccgcacg cgggcggaaa gtccctccgg
     1801 gagaattcga gggacgttgt ccgtaatgcc gcctccggtg acgtgagcca ttgcgtggat
     1861 cattccgcgc gcgatcagcg gcaaaagtgc gtggagataa catcggtgtg gggcaagaag
     1921 tgcgtctgca accgtcctgc cccattccgg aacgaatgtg tctgtgttcc agccggcgat
     1981 atcaaagatc aacttccgtg cgagcgaata tccgtttgta tgcaggccgt ccgacatcaa
     2041 gccgatcagc acgtcgccat ggcgcacgtt agcgccggtg atgacttctc gttttcccac
     2101 aactccgacg atagtccccg cgaggtcgta ctccccgggc ctgtacatgt ccggcatctc
     2161 tgccgtttct ccgccgagca gagcgcaccc gactgcagtg cacgcagcgg acaagccctc
     2221 gaccacgtcg acgacaatct gcgtgtcgtg tctccccagc gcgatgtaat ccataaagaa
     2281 gagtggacgg gcgcccatga cgaggatatc gttcacgcaa tggttcacca gatccatccc
     2341 gacagtggtg tgacggcccg ccactacggc cactttgagt tttgtgccca ctccgtctgt
     2401 gcttgcgacg agaacagggt cctctacgtc cccgaaatcc gcctcaaaca agccgccgaa
     2461 acttccgagc tcggtcagta cctgtggcgt atagcttttg cgaacaagct ctttgaggag
     2521 ttccttcgtt ctgttcgctt tttcgatatc cacacctgcg tccgcgtagg ttaccgtccg
     2581 caacggttcc gcagggctca ccgccaggtg acgtgcgaac cacgaaacag tgcgatcgat
     2641 tacctgttcc tccgtgcccg cactgccgaa aaggtggtct gcgccatcga tcagaacacg
     2701 ttcggatggg attcccgtcg aagcccgcgc atcgataaac gattgacttg tgctggctgg
     2761 caccgcctgg tcattggtgc cgtgaagaat cagaaccggg gcactcgaac gggcgagcga
     2821 ggcgaccgga tcaacgcgag gcaattcgtc cacaaatgct cgccccagaa acaaacctgg
     2881 cgagaactcg tgtgggaacg tgattccttc ggtgagaaga ggtgcgaact gaacgtccgg
     2941 gtcgcttacg ggagcccaca ggaccaccgc tttcaagagc gggatatccc ccgcaacaca
     3001 cgcggctaca catcctccga gcgagaatcc aaggatgccg attcgcgcgc gatccacccc
     3061 gtcacaggtt gagagaaagg ttacggccgc tttcgcgtcg gagacctcgt ccgaaatcgt
     3121 catgttacgg aactcgccct cgctgtcgcc ggatccccgg aaatcgaaac ggaggactgc
     3181 aatacccgaa ctcgcgagac gccgtgcgag acggacgaat atccaccgcg cctcgctttt
     3241 tgttccggtg aacccgtgac aaagcaccac accgggtact gtcccgtcca gatcggggcg
     3301 atgcagaatg cccacgattt gttgaccgtg gttctgaaac gaaacgagtt tttccatttg
     3361 atgcttcagt ccttccagtc aggcttgacc gggttcagcc ggtcagaacc tcgggattga
     3421 cgaggtttgg cggtgtgtcg ccgcggaggg cggccagcag attcgtggcc gccatgacag
     3481 ccatcttgtt ccgagttgca attgtggcac ttgcagtatg cggcgtaaga acggcatttt
     3541 tgcagtccgc taaaccagga gcgagctgcg gttcatcttc gtacacgtcc aaccccgcgc
     3601 ccgcaattga acccgatttc agggcctcca ccagcgcacg ctcatcaacc accgggccgc
     3661 gagatgtgtt aaccaggata gcgccatttt tcatcagggc gaattcccgc ttgccgatta
     3721 gatggcgcgt ttcctccagc agggggacat ggagggtaac gatatcggat gccttaagaa
     3781 gctcgtcgag atccaccctt atcgcgttca ggctcgatat ctccgggttg ggggcggagt
     3841 ctgtgtaaag caccttcatc ccgaatccct gcgcccgcct ggccaccgca cagccgattc
     3901 gtccggcgcc gacaattcca agcgtacttc cgaatacgtc catgccaagc agcatcattg
     3961 gtccccaccc gtggaactcc ccggcgcgga cgagcctgtc tccttcaaca atcctgcgtg
     4021 ccgtcgccat gatgagcgcc caggccatgt ccgcagtagt ctccgtaagc acgccgggcg
     4081 tgtttgttac cataatgccg cgtttcgtag cggcggctac gtccacgttg ttgtagccca
     4141 ccgcatagtt cgcgaacacg cgcgcgccgc gggcagcttc caggaccgat tcgtccacaa
     4201 tatctgtcaa caggcacaga actccgtccc ttccgctcac acccgctaac aactcctcat
     4261 gcgtcagcac gcggtcttcc gggttgactt cgaagttcac accggcgttg cggagcagat
     4321 cgagacctgc gtccggtata cggcgcgtga cgaacacatt ccatgttggc atcgtttttc
     4381 cctttttcag ttgactagac cggttcttct tttctgcttc cttcgtcagg aacaacgcgc
     4441 atggcggttc cagccttgca cgttttcttt cggtattcct cgacgacttc ctccgcgaac
     4501 acgtctgccg aacctgtgtc caccaaagaa atcacagcgc ccccgaaacc cgctcccgtg
     4561 aggcgcgccc cgtaacatcc cggggtggaa gaggcgattt ccacgagaag atcaatctcg
     4621 gggagactt
//
LOCUS       DAOTNG010000258         4628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15249_length_4628_cov_4.401924, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000258 DAOTNG010000000
VERSION     DAOTNG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15249_length_4628_cov_4.401924"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..841
                     /gene="tsf"
                     /locus_tag="P5179_11340"
     CDS             248..841
                     /gene="tsf"
                     /locus_tag="P5179_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRS95853.1"
                     /translation="MEITAAQVKELREKTGAGMMECKKALGETNGDLQKAIDYLRQQG
                     ILKSAKLGSRATSQGAIFSYVHPGDQLGVLVEINCETDFVARTDDFKALGKNIAMQVA
                     ASAPQYVSRDQVPQEVLDHEKEVSRAQAKAEGKPEKILDKIVEGRLEKYFQEVCLLDQ
                     AYIRDDSRKVSDLVTEAIAKIRENITIKRFVRFKVGE"
     gene            897..1610
                     /gene="pyrH"
                     /locus_tag="P5179_11345"
     CDS             897..1610
                     /gene="pyrH"
                     /locus_tag="P5179_11345"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRS95854.1"
                     /translation="MSACKRVLLKLSGEALQGSHAFGIDPVEVSRICQEIVEVHSRGV
                     QIGLVVGGGNIFRGKEIVNQLGVEAASADTMGMLATVINCLALQAALEKLGLETRVQT
                     AIRMDQVAEPLIRRRAIRHLEKERVVLFAGGTGSPFFTTDTAAALRAVEIGAEVLLKG
                     TKVDGVYDDDPARNPGANLFDEITYEDILQHGYKVMDATAVSLSMDNRLPIVVFNLTR
                     AGELVRAALGDRVGTRVVA"
     gene            1636..2199
                     /gene="frr"
                     /locus_tag="P5179_11350"
     CDS             1636..2199
                     /gene="frr"
                     /locus_tag="P5179_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRS95855.1"
                     /translation="MATESQIVRQTRAKMQKTLETIVHEFSTVRTGKANPALLDVVRV
                     EAYGSKVPISQVANVTAPEPAMLLVQPWDKSTIGAIDKAIRLSDLGLNPTNDGNVIRI
                     ALPPLTEERRKEYVKIVHKMAEDGRIVIRNERRDAIEELRKMEKAKQVSEDDSRRDQK
                     EIQTLTDEFIKKVDAALAAKEKEIMEV"
     assembly_gap    2510..2609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2610..3235)
                     /locus_tag="P5179_11355"
     CDS             complement(<2610..3235)
                     /locus_tag="P5179_11355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS95856.1"
                     /translation="MPAFAGRTALVTGASKGIGYHIARLCAREEADVILVARDVARLE
                     RAAEEITGSFPVKAAVFPADLSKPDSAAELWHRVQSAGHSVDILVNNAGIGAYGEFHE
                     ISPEKTRGMRHLNIVSFTELMHLAAVEMARRKWGRILTVASVAGFQAMPYMAEYAATK
                     AYVLSLSEALRVELAPYGVVVTCLAPGPTDTDFFSSAGTTMLNTPGRKT"
     gene            3357..>4628
                     /locus_tag="P5179_11360"
     CDS             3357..>4628
                     /locus_tag="P5179_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95857.1"
                     /translation="MQHSVASFGRDLLQVDYASYLSQHDVVYETPPAIGCHGLPIGNG
                     VSGALVWTPPTAVRWELGGSDLWADGPARPFGSWNKEDEEVATALLSAGSLSIGYDIP
                     AFDWAYLKKFSARLSLFPAEVSLNAETPFSRVRMKTWMNSDPSCLIIQWSDDLDDAIP
                     RKITLERWGSRVFAHWYRTIVRDPSRGLGGTEASVHGDVIILRHSSRSHDFVVAAKVT
                     GVPSVARAAHRHAAVADVAAVRSAEFTVLLTIASSETTGDPLQTALERIANAEAAGVS
                     NLQTGHRRWWAGFWERSFVNIPDAYLENLWYINQYVMGSSSRGDYPPHFINGIWNWNR
                     DFRAWNHFYHWNQEELNWPVFASGHPELALGYLKMRAKALPLAQQSAMRKYGIDGAWY
                     SDVFERRGWQDEGVPLFTPGLQIALDMWRYWR"
BASE COUNT          959 a         1230 c         1402 g          937 t
ORIGIN      
        1 gttctggcgg acgcagtcat tgaagggatg cagaatatcc agcagccgga acaggctgaa
       61 ccggcggcgg tgagccgtga atcgggcgag cgagagcgtt cccgccgtgg cggaccacgt
      121 cgcccgcgcg gcgcccgttc tggctctgaa gccgttgcag cctgatttgc actgaaataa
      181 cggcggaagg ggccacgacg tccctccgcc gcatttgttg taccgtctct ttgttacgag
      241 gaacacaatg gaaattaccg ccgcacaagt gaaagaactg cgagagaaga ccggcgccgg
      301 catgatggag tgcaagaagg ctctcggtga gacgaatggc gaccttcaga aggcgattga
      361 ctatctccgc cagcagggga ttctcaagag tgcaaagctg gggtcccgcg ctacaagtca
      421 gggcgcgata ttcagttatg ttcatcccgg cgatcaactt ggtgtgctgg tggagatcaa
      481 ctgcgaaact gatttcgtcg cccgcacaga tgacttcaag gcgctgggaa agaacatcgc
      541 aatgcaggtc gcggcgtctg ctccccagta tgtgagccgg gatcaggttc cacaggaagt
      601 cctcgaccac gagaaagaag tatcccgcgc ccaggcgaaa gcggaaggga agccggagaa
      661 gattctggac aagatcgtag aagggcggct cgagaagtat tttcaggaag tgtgcctgct
      721 cgatcaggcg tacattcggg atgactcccg caaggtgtcg gatctggtga cggaagccat
      781 tgcgaagatt cgcgagaaca tcacgatcaa gcggttcgtg cgtttcaaag tgggtgaatg
      841 agcagctttg acgttccgcg tcactggttt tccacattcc aacgacggaa gtccccatgt
      901 cggcgtgcaa acgggtactg ctgaagctgt ctggcgaggc tctgcaggga tctcacgcgt
      961 tcggtattga ccctgtggag gtcagccgca tctgtcagga gatcgtggag gttcactccc
     1021 gcggggtcca gatcggcctt gttgtgggag gagggaatat tttccgcggg aaggaaatcg
     1081 tcaaccagct tggagttgag gcggccagtg ccgacaccat ggggatgctt gcaacggtga
     1141 tcaactgcct tgcgctgcag gccgcgctgg agaaactcgg tctggaaacg cgcgttcaga
     1201 cggccatccg tatggaccag gtggcggagc cgctcattcg ccggcgcgcc atccggcacc
     1261 ttgaaaaaga gcgcgtggtg ctgtttgccg ggggaacggg cagccctttc tttaccacgg
     1321 atacggctgc agcgttgcgc gccgtggaaa tcggcgcgga ggttctgctg aaaggcacga
     1381 aggtggatgg cgtgtatgat gatgatcccg ccagaaaccc gggtgccaac cttttcgatg
     1441 aaatcacgta cgaggatatt ctccaacacg gttataaggt catggatgcc acggcagtca
     1501 gtctttccat ggacaacagg ctgccgatag tcgtgttcaa cctcacccgc gccggcgaac
     1561 tcgtgcgggc agcattgggc gaccgtgtcg gcacgagagt agtggcgtag gcgaaccgga
     1621 acaagggagg tgcagatggc aacagaaagc cagatcgtcc gccagacgcg ggcgaagatg
     1681 cagaaaactc tcgaaacaat tgttcacgag ttcagtactg tcagaacggg caaggccaat
     1741 cccgcgcttc tggatgttgt ccgcgtagaa gcgtacggct cgaaggttcc gatttcgcaa
     1801 gtggcgaatg tgacggctcc cgaacctgcg atgctgctgg ttcagccgtg ggacaagtcc
     1861 accattggtg cgatagacaa agccatcagg ctttccgatc tgggtttgaa tcccacgaat
     1921 gatgggaatg tcatacgaat tgcgctacca cctctcaccg aggaacggcg caaggaatac
     1981 gtgaagatcg tgcacaaaat ggcggaggac ggacggatcg taatccgcaa cgagcgtcgt
     2041 gacgcgattg aagaactgag aaaaatggag aaggcgaaac aggtttcgga ggatgacagc
     2101 cggcgcgatc agaaggaaat ccagactctg acggacgaat tcatcaagaa agtcgatgcc
     2161 gcgcttgccg caaaagagaa agagatcatg gaagtgtgag cagacgccat agaaaagtta
     2221 cgtgaaggcg cgcgggggat accgcgcgcc ttttttgaaa ccgcactccc gtttgtccct
     2281 gaatactgct cgcgctactc ttcacctggc aattgtccgc cctcgacact accggagata
     2341 caaccatgcc gttcccgatg acgagtattg aacatgttca ggtctcgcgt gttctttgcg
     2401 gtacgaaccc tttcttcggg ttttcccact tcagcgcggc gagagacgcg tggctgcgca
     2461 accatttcac gaaggaacgc atcctcgagg tcctcgaagc gtgccgtctn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttttccttcc cggcgtattc agcatggtgg
     2641 ttcccgccga agagaagaaa tccgtatcag tcggtccggg tgcaaggcac gtcaccacga
     2701 ctccgtacgg agcgagttcc actcgcaggg cttccgaaag agaaagcacg tatgccttgg
     2761 tggcggcgta ttccgccatg tacggcatgg cctgaaaacc cgcgacagag gcaacggtga
     2821 ggatgcggcc ccatttccga cgggccatct cgacggccgc aaggtgcatg agctcggtga
     2881 acgaaacgat attcaggtgc cgcattccgc gtgttttttc cggcgagatc tcatggaact
     2941 caccgtatgc gcctatgccg gcattgttga cgagaatgtc aacactgtgt cccgctgatt
     3001 gaacccgatg ccacagttct gctgcggagt ctggtttgct gaggtcggcg gggaacactg
     3061 ccgctttgac ggggaagctc ccggtaatct cttctgccgc gcgttcgaga cgcgcaacgt
     3121 ccctcgcgac caggatcacg tccgcctctt cacgtgcgca aagccttgcg atgtggtaac
     3181 cgattccctt tgaagcgccc gtcacgagcg ctgttctgcc tgcgaatgcc ggcatgcgat
     3241 ctccttgtcg cggattccac ccggagccga tttcaggtat tcttaatttc gtgttttttc
     3301 tcccagcgag ttcccccgcg tgaatggtga tcacccccca acggagattc gggtacatgc
     3361 aacacagcgt cgcgtcgttc ggcagggatc ttcttcaggt ggattacgcc tcttatctca
     3421 gccagcatga tgtggtgtat gaaacgccac cggcgatcgg atgccacggg ctgcccatcg
     3481 gaaacggggt ttcgggcgca ctggtgtgga ccccgccgac ggcggtacgc tgggagctgg
     3541 gcggttcaga tctctgggca gacggtcccg cgcgaccctt tggatcatgg aacaaagagg
     3601 acgaggaggt tgctactgct ctgctctcgg cgggttcact ctcgatcggt tacgacatcc
     3661 ctgcgttcga ctgggcgtat ctgaagaagt tctccgcccg cctctctctt tttccggctg
     3721 aggtgtctct caacgcggaa acgccgtttt cacgagttcg catgaagacg tggatgaaca
     3781 gcgatccttc gtgcctgatc atacagtggt ccgacgatct ggacgacgcg atccctcgaa
     3841 agatcacgct ggaacggtgg ggaagccgtg tgttcgcgca ttggtaccgt accatcgttc
     3901 gggacccctc tcgaggtctc ggtggaaccg aggccagcgt gcacggcgac gtgattattc
     3961 ttcggcacag ctcccgctcg catgactttg ttgttgcggc aaaagtaaca ggtgttcctt
     4021 cggttgcacg ggcagcgcac cgccatgctg ctgtggcgga tgttgcggcg gttcgttcgg
     4081 cggaattcac cgtcctgctt acgattgcct ccagcgagac caccggagac cctctgcaaa
     4141 ctgctctgga acggatcgcg aatgcggaag cggcgggagt ctcgaatctc cagaccgggc
     4201 accgccggtg gtgggcgggt ttctgggagc gctcgttcgt gaacatccct gatgcgtatc
     4261 tggaaaacct ctggtacatc aatcagtacg tgatgggctc gagttcgcgg ggggattacc
     4321 cgcctcattt catcaacgga atctggaact ggaatcgcga tttccgcgcg tggaatcact
     4381 tttaccactg gaatcaggaa gagctgaact ggccggtttt cgcatcgggg catcccgaac
     4441 tggcgctcgg gtatctgaaa atgcgtgcaa aggcccttcc gctggcacaa cagagcgcaa
     4501 tgcggaagta cggaatcgac ggtgcgtggt acagcgacgt gtttgagcgt cgtggctggc
     4561 aggatgaagg cgtgccgttg ttcacgccgg gccttcagat tgcgctcgac atgtggcggt
     4621 actggcgg
//
LOCUS       DAOTNG010000259         4570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15479_length_4570_cov_4.652935, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000259 DAOTNG010000000
VERSION     DAOTNG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15479_length_4570_cov_4.652935"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="P5179_11365"
     CDS             complement(<1..738)
                     /locus_tag="P5179_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRS95858.1"
                     /translation="MELHARTNCIVEIPFRSAAVYRNPYADVTLDVAFSNSRGAKIIV
                     PAFWAGGATWKVRFSVPSPGTWKFQSICDNRSDIGLHGRTGAISVQRYRGSNPVLKHG
                     RVRVSPNGRYLEHQDGTPFFWLGDTWWMGLCTRVDWPQGFKELTADRVAKGFSVVQII
                     AGPYPDMDAFDPRARNEAGFAFERGFTSVNPSYYDHADLKIGHLVESGLVPCIVGMWG
                     YYLPEIGVEQIKRFWRYLIARYGAYPVV"
     gene            complement(792..2384)
                     /locus_tag="P5179_11370"
     CDS             complement(792..2384)
                     /locus_tag="P5179_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRS95859.1"
                     /translation="MEMNEQEQKIAAAREMRERLLRDRYRPGYHFAIPEDIGMPGDPN
                     GAFWANGRYHLMYLYNRRGTKAWGNAAFCWGHISSADLVHWRHHADALLPGDGDGGCF
                     SGGAFVDEDGTAYLTFWRLPLADGKTEGTGIGIARSRDRNYDTWEKMPVPTLDGTEFG
                     IRETRDSEGKAYPLCNADPSNIWKKNGTYYLQTGNLLVLRKMEKSDDASAKWKGDWVD
                     LFRSRDLVDWEYVHRFYARNPTNRWTDESEDDMCPSFLPLPSSPSGGFPSGKYLQLFI
                     SHNKGCQYYIGTYDEGRDLFLPETHGRMTWVDNTFFAPEALIDDKGRQIMWAWLLDNA
                     PSEPEQQILTGWSGVYCLPRTLWLGEDGMLRMAPVPELSVLRYNPRVFDDVNLHPGSE
                     KTLEVANGESAEINLSIESLTATQAGLKVRTSPHDEETTLLYCDFEHNKLVFDSTRSG
                     NAGRKVKEEAPFECRKGERIDLRVFIDRAVVEVFAGDRQAIARRVYPVRDDSTGVRLF
                     CVGGEVAFTTIRTWEMMPSNAW"
     gene            complement(2468..3340)
                     /locus_tag="P5179_11375"
     CDS             complement(2468..3340)
                     /locus_tag="P5179_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95860.1"
                     /translation="MKIAFHQITSGPKRDLKETFKAYAEAGWRHFEVNLWETEAFVTQ
                     HGAKSLAALAKDFGLHCVGATGLSIPAFQGGEAHEAATKQMEIHAQVMNALECTTIVV
                     GGDVPKDFPPRAQNSTEKALSARDATYRNALKEFAMAVERVAETAAKYDVTLALEVNW
                     CGLARSFRTMAELVMMVDRDNVGATWDPAHFYSTASRLSDLDRLRGKIVHAHLNDIRN
                     CFMEAMDINGDRVLPGDGVLPLVEWTDKIHSLGYRGWHCVELFSDDLWAEPLHEIATR
                     AKKACERVWPDAEF"
     gene            complement(3358..4392)
                     /locus_tag="P5179_11380"
     CDS             complement(3358..4392)
                     /locus_tag="P5179_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRS95861.1"
                     /translation="MIDIRSDTVTKPTAAMRAFMANAEVGDDVYREDPMVRALEEETA
                     ALLGKEAALYVPSGTMSNQIGVWVNTHEGDEVICERDCHIFNYEAGAPALLSRVLMKT
                     LQGARGVISAAQVEEAVNPANIHAAATTLVCLENTHNKAGGTIYPREEMERISDLCKR
                     GDMRLHLDGARLWNASAATGIPERDYAELADTVSVCFSKGLGAPVGSALCGAQEVIER
                     ARRKRKILGGGMRQAGIIAAGALYALRNHRVRLVEDHANARCLAEAINELPGIKVDLE
                     GVQTNIVMIEITGNATAQQVSAMMKDEGVLINAMRPKVLRAVTHLDVSPRDIEKAIEA
                     FARISRRIRS"
BASE COUNT          891 a         1435 c         1231 g         1013 t
ORIGIN      
        1 tacaacggga tacgcgccgt accgcgcgat gaggtaccgc cagaaacgtt tgatttgttc
       61 gaccccgatt tcggggaggt aataccccca cattccgacg atacagggaa cgagcccgga
      121 ttccacgaga tgtccgattt tgagatccgc gtggtcgtaa tatgacggat tgacagaggt
      181 gaaccccctc tcaaacgcaa aaccggcttc attccgagcc cgtggatcga acgcatccat
      241 gtcggggtag ggtcccgcga taatctggac cacgctgaac cctttcgcaa ccctgtccgc
      301 ggtcagttct ttgaacccct gtggccagtc aacccgcgta caaagtccca tccaccacgt
      361 gtcaccaagc cagaaaaacg gtgtgccgtc ctggtgttcc agatagcgcc cgttcgggga
      421 aacacgcact cgcccatgtt tcaatacggg attactgcct ctataccgtt ggacagaaat
      481 cgcgccggtt ctcccgtgca gaccgatgtc tgaccggttg tcgcagattg actggaactt
      541 ccaggttccc ggagaaggaa cggaaaaccg gactttccag gttgcgccgc cggcccagaa
      601 ggcaggaacg atgattttcg cgccccggga gttcgagaac gccacgtcca gagtaacatc
      661 cgcgtaagga ttcctgtaca ccgcggcgga acggaaaggt atctctacga tgcaattcgt
      721 acgtgcgtga agttccattg tctttctccc cgccacccgg gcgacttgcg cactgcgctc
      781 ttttcccccg atcaccacgc gttcgacggc atcatttccc atgttctgat cgtggtaaat
      841 gcaacttcgc ctcccacaca gaacaggcgc acgccggtac tgtcatcgcg aaccgggtag
      901 accctgcgag cgatcgcctg gcggtccccc gcgaaaactt ccaccaccgc cctgtctatg
      961 aaaacacgca gatcgatgcg ctcgcccttg cggcactcaa agggcgcttc ctctttgact
     1021 ttccgtcccg cgttcccgct ccgtgtagaa tcaaacacca gcttgttgtg ttcaaaatcg
     1081 cagtacagca acgtcgtttc ttcgtcatga gggctcgtcc ggactttcag acccgcctgc
     1141 gtcgcggtca acgattcgat ggacaaattg atctccgccg actcgccgtt ggccacttcc
     1201 agggttttct cagaacccgg gtggaggttc acgtcgtcga aaactcgggg gttgtacctc
     1261 agcaccgaca gttcgggtac cggcgccatt cgcagcattc cgtcttcccc gagccagagg
     1321 gttcttggaa ggcaatagac tccagaccac cccgtcagaa tctgttgttc cggttcggaa
     1381 ggcgcgttat caagaagcca tgcccacatt atctggcggc ctttgtcgtc gatcagcgct
     1441 tccggagcga aaaacgtgtt gtcaacccag gtcattcggc cgtgtgtctc cggcaggaag
     1501 aggtccctcc cctcgtcata cgttccgatg tagtactggc accctttgtt gtgggaaatg
     1561 aagagctgga ggtatttgcc gctcggaaag ccaccagaag gcgaactcgg aagcgggaga
     1621 aagctggggc acatgtcgtc ttcgctttcg tccgtccacc tgttcgtcgg gtttctcgcg
     1681 tagaacctgt ggacgtactc ccaatcaaca agatcccttg accggaacag atcgacccag
     1741 tcgcccttcc atttcgccga ggcatcgtca gatttttcca ttttcctcag gacgaggaga
     1801 ttgcccgtct ggaggtaata cgtgccattc tttttccaga tattgctggg gtcggcgttg
     1861 cacagggggt acgccttccc ttcgctatcg cgggtctccc gaatgccgaa ctcagtccca
     1921 tcgagcgtgg ggacgggcat cttttcccag gtgtcgtaat tgcgatctct acttctcgcg
     1981 attccgatcc ccgtgccctc cgttttcccg tcggcaagtg gcaatcgcca gaacgtcaga
     2041 taggcagtcc catcctcgtc cacaaaagcg ccgccgctga agcagccacc atcgccgtcg
     2101 ccgggaagaa gcgcgtcagc gtggtgtcgc cagtgaacga ggtctgcgct ggaaatgtgg
     2161 ccccagcaga acgccgcatt gccccacgct tttgttccgc gccggttgta cagatacatg
     2221 agatgatatc ggccgttggc ccagaatgcg ccattggggt cgcccggcat cccgatatct
     2281 tccgggatgg cgaagtggta gccgggccga taccggtcgc gcaacaggcg ctccctcatc
     2341 tcccgggctg cggcgatctt ctgttcctgt tcattcattt ccatgagaag ccttttcctt
     2401 ttccgtccat ctcgccgctg caacaggcct acctgcggga actcatccgt tccttttcga
     2461 ctggagatca gaactccgcg tctggccaca cgcgttcgca agcctttttt gcccgcgtcg
     2521 caatctcgtg cagcggctcc gcccagagat cgtcgctgaa caattccacg caatgccagc
     2581 cacgataccc gagggaatga atcttgtccg tccattccac gagcggcaac acaccgtcgc
     2641 cgggcagcac gcggtcgccg ttgatgtcca tggcctccat gaagcagttt cggatatcgt
     2701 tgagatgggc gtgcacgatc ttgcctctca ggcggtccag atcggaaagg cgcgacgctg
     2761 tgctgtaaaa gtgcgcgggg tcccacgtgg cacccacgtt atcccgatca accatcatca
     2821 ccagctccgc catcgtccgg aagcttctcg ccagaccgca ccagttgacc tccagcgcca
     2881 gtgtgacgtc gtatttcgcc gcggtctccg ctacccgttc gaccgccatc gcaaattcct
     2941 tcagtgcatt cctgtacgtg gcatcgcgtg ctgaaagcgc tttttccgtc gaattctgcg
     3001 cgcgaggagg gaaatccttc ggcacgtcgc cgcccaccac aatcgtggtg cactcgaggg
     3061 cattcatcac ctgcgcgtga atctccatct gttttgttgc agcctcgtga gcttctccac
     3121 cctgaaacgc ggggatgctg agacctgtcg caccgacaca atggagcccg aaatccttcg
     3181 cgagagcggc cagcgacttc gcaccatgtt gagtgacaaa cgcctccgtt tcccagaggt
     3241 tcacctcgaa gtggcgccat ccggcttccg cgtaggcttt gaacgtctct ttcaggtctc
     3301 tcttgggccc cgaagtaatc tgatgaaacg cgatcttcat ggaagactcc tccaagttca
     3361 actccggatt ctgcgactga tccgcgcgaa ggcttcgatc gccttttcga tgtccctggg
     3421 actgacgtcg agatgcgtta ctgcccgcag gactttcggt cgcatcgcat tgatgagaac
     3481 accttcatcc ttcatcattg ccgaaacctg ctgtgccgtc gcgttccctg tgatctcaat
     3541 catcacgatg ttcgtctgga cgccctcaag atcgaccttg ataccgggta actcgttaat
     3601 tgcttccgca agacagcgcg cattcgcgtg gtcttcgacc aggcgcacac gatggttcct
     3661 cagcgcatag agcgcaccgg cggcgatgat tcctgcctgt cgcataccgc caccaagaat
     3721 cttgcgtttt cgacgtgcgc gttcaatcac ctcctgtgcg ccacagaggg ccgaaccaac
     3781 gggtgcgccc agaccttttg agaaacacac ggatacggtg tccgccagtt ctgcataatc
     3841 gcgttccggt attccagtgg ccgcagaggc gttccacaac ctcgcgccgt caaggtggag
     3901 ccgcatgtcc cctcgtttgc acaagtcgct gatgcgctcc atttcctcac gcggatatat
     3961 cgtgccgccg gctttgttgt gcgtgttttc cagacacacg agggtagtag ccgcggcgtg
     4021 gatattggca ggattcactg cctcctccac ctgcgcggcc gaaatcaccc cgcgtgctcc
     4081 ctgcaacgtt ttcatgagaa cccgagagag cagcgccggc gcacccgctt cgtaattgaa
     4141 gatgtggcag tcccgctcgc agatcacttc gtcgccttca tgggtgttca cccatacgcc
     4201 gatctggttg ctcatggtgc cggaggggac gtagagtgcg gcctccttgc ccaacagggc
     4261 ggccgtttcc tcctccaatg cgcggaccat cgggtcttcg cggtacacat catctcccac
     4321 ctcggcgttc gccatgaacg cgcgcatggc agcagtgggt tttgtgaccg tgtcgctgcg
     4381 gatatcaatc atgttgcttc ctcaaccaat gaaggaaatc ggcccgggaa tgctgaccgg
     4441 acggtgacag cctgtatgga gaaggaaaat aacgcgggac ttgccgaggg gctagacagg
     4501 ggtggggcgg agcgtggctc cccagcggaa acggatggtc aaaacgagct gctgaatctt
     4561 gtactcgtgg
//
LOCUS       DAOTNG010000260         4569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15484_length_4569_cov_4.647541, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000260 DAOTNG010000000
VERSION     DAOTNG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15484_length_4569_cov_4.647541"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..317)
                     /locus_tag="P5179_11385"
     CDS             complement(27..317)
                     /locus_tag="P5179_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS95862.1"
                     /translation="MTTTKRDLAKYVAKDTGCKQSLAARMVDSVFEGMRESLMRGDRI
                     EIRGFGVFEVRNTRPKPAARNPRTGDIIQVPARRKTHFKPGKILKEELRRPR"
     gene            complement(596..1681)
                     /locus_tag="P5179_11390"
     CDS             complement(596..1681)
                     /locus_tag="P5179_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95863.1"
                     /translation="MTHRERFNRIFRYQPVDRAPYWGTGGWSETMRRWVSEGMPEGVS
                     LSKYFGAEETKGIPVYYGPWPHFPYEVLKEEGATQVVRNHEGIIMRVYKTDGDRSMPH
                     FLEFPVKTRDDYRTIIKPRLCQNPRDRFPADWNRRCAEWKERTDPLCLFGDRWSGFFG
                     PLRNLMGLENLSYAFYDDPALIEEMMDDICDNILLITEQILNDTDIDYWAFWEDMGMK
                     TGPLLSPELFDRFMVPRYRRVTDYLRSRGVDLIFVDSDGDVHALIPLWLKAGVNGLWP
                     CEIAANMEPAPIKKQYGRDLLLCGGIDKRALAAGPKAIDEELKKVPPLVVGGGYVPMV
                     DHSCPPDISWSNYCYYMEKLKEAIRIA"
     gene            complement(1722..2840)
                     /locus_tag="P5179_11395"
     CDS             complement(1722..2840)
                     /locus_tag="P5179_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95864.1"
                     /translation="MTSKERILRALHREKPDRLPVSVHQWQGYHLQTYMGGITDIEAF
                     AATGMDAQIQYFQSMGQFWLVDADFSKTSTPQWRDDVQVLSNDPDHRKARHVITTPEG
                     ILTYETEGDRKTTWITKYLIEHDEDIELIEKYMPVAVFDPAPVAQLYEEIGDRGIIRG
                     FVWGDQAGCWQHACCLEDVNRLILACFDKPDWVHRLLRSLLEKKLQFIAGLKGARFDL
                     IETGGGAASSTVISPDLHREFCLPYDRALHDALHDLGFLVTYHTCGGTRGIEELIVEN
                     GCDVSETLAPRSIGGNQEPWEFAEKIRNRVALVGGMDQHNCLTTGTPQSIRQHVHTLF
                     ERVGRNGGYVLSTADHFFDTPVENLNAYAAAARECTYS"
     gene            complement(2990..3970)
                     /locus_tag="P5179_11400"
     CDS             complement(2990..3970)
                     /locus_tag="P5179_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95865.1"
                     /translation="MLVRWGFLSGAWIWPVLFAIGCGTTGPDLVAPATPAGFGRIGGG
                     DGENRFGWQRNREKDLAGYRMYRAEGDPAAAYQLIATISRDSTSYTDRNLDYTIQFYY
                     KMTAFDNAGNESPATLPILAIAANLTAPSVPANVYAVGQNLVPPASISVSWSANPEAD
                     FAEYWVFRSVTTPAPYVGTPVAIVPRGTTSFNDTTVAVGTRYYYRVIAVDKGGLKSPG
                     SQSAEVSDVALPPATLAAPGNNATTASLTPEFRWVAVPQAVGYKLIIYADAGGFTQHG
                     TAYANSPATSIIYAGTPLISGRTYYWRLAATTKGPDQINSISTMWRFTAP"
     gene            complement(4169..>4569)
                     /locus_tag="P5179_11405"
     CDS             complement(4169..>4569)
                     /locus_tag="P5179_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95866.1"
                     /translation="RQAVQIDPTNFTALVNLGNTLFDLEQYAAAESALVRAESLRPRD
                     FRPPLKLAETYMKLNKCGEAEQAATRALAVQPGNASAHATRAEALECLGRTREALDAY
                     VQASRDPRWKQYADRKAKTLREQLGLPAQN"
BASE COUNT          915 a         1402 c         1280 g          972 t
ORIGIN      
        1 gtcagtgcgt caattacgag gaaagtctac ctcgggcgac gcagttcttc cttcagaatc
       61 ttgccgggtt tgaagtgtgt tttgcgccgc gcaggtacct gaatgatgtc gcccgtccgt
      121 ggattccgcg ctgccggttt cgggcgcgtg ttgcgcacct cgaaaacgcc aaacccgcgg
      181 atttcaatcc ggtcaccgcg catcaggctc tcgcgcattc cttcaaacac actgtccacc
      241 atcctggccg cgaggctttg tttgcaaccg gtgtcctttg ccacgtattt cgcaagatca
      301 cgcttcgttg tcgtcatacc gggaactcct tagtttgtcc tcgcggcagg gactatgccc
      361 cggggacgct cacgcgacac gttcggaagc ttgctgcggc ggcgtttttc cggatcgatc
      421 tttcccagcc tcgcggcttt tgtaatgccg atcacaccgt acagcctaag atcaaagtaa
      481 acactcgctt cccgttagtc aagagatgcg atcgcggatt cggaacgcgg aggcatcctc
      541 cggtgtgcat cacaagggat tgatgacctc ggaggaaacg gccgtgcgcg cgattttacg
      601 cgattcggat cgcttccttc agcttttcca tgtaatagca gtaattggac caagagatgt
      661 cgggcgggca ggaatggtcc accatgggga cgtacccacc tccgacaaca agaggtggaa
      721 cctttttcaa ttcctcatct atcgctttgg gcccggccgc aagcgcccgt ttatctattc
      781 ccccgcacag gagcagatcg cgcccgtact gcttctttat cggtgccggt tccatgttcg
      841 cggcgatctc acagggccag aggccgttga ctccagcttt cagccacaac ggaatcagag
      901 catgcacgtc gccgtcggaa tccacaaaaa taaggtcaac gccgcgcgat cggaggtaat
      961 cggtgaccct gcggtaccgg gggaccatga accgatcgaa caactcaggg ctgaggagag
     1021 gcccggtctt catgcccatg tcctcccaga acgcccagta atcaatatcg gtatcattca
     1081 agatctgctc tgtgatcaac aggatgttgt cgcagatgtc atccatcatt tcctcgatca
     1141 gcgcgggatc gtcgtaaaag gcgtacgaca ggttttccag ccccatgaga ttccgtagcg
     1201 ggccaaaaaa accggaccaa cggtcgccaa agaggcaaag ggggtcggtt cgttccttcc
     1261 actcagcgca ccgccggttc caatcagccg gaaatctgtc cctcgggttc tgacagagtc
     1321 gcggcttgat gattgtccgg tagtcgtccc gcgtcttcac ggggaattcc aggaaatggg
     1381 gcatactccg gtcaccatcg gttttgtaga cccgcatgat gattccttcg tggttcctca
     1441 caacctgcgt tgcaccttcc tccttgagaa cctcatacgg gaaatgcggc catgggccgt
     1501 aatacaccgg aatacccttt gtctcctccg caccgaagta tttggataga ctgacgcctt
     1561 caggcatacc ttcggaaacc catcggcgca ttgtctctga ccatccacct gtgccccagt
     1621 acggggcacg gtctaccggc tgataacgga aaatccggtt gaagcgctct cgatgcgtca
     1681 tgttggttac ctttccggcc agatccacca gggaaaacgc ctcaactgta cgtgcattcc
     1741 ctcgcggcgg ccgcatacgc gttcagattt tccacagggg tatcgaaaaa atgatccgcg
     1801 gttgaaagga catatccgcc gttcctgccg accctttcga acagcgtgtg cacgtgctgg
     1861 cgaattgatt gcggcgtgcc ggttgtgagg caattgtgct gatccattcc accgaccaag
     1921 gccacgcggt tccgaatctt ctccgcgaat tcccagggct cctgattgcc cccgatactt
     1981 cgtggcgcaa gtgtctcgga aacatcgcag ccgttttcca cgataagctc ctcaatgcca
     2041 cgcgtgcctc cgcacgtgtg gtaggtgacc agaaatccaa ggtcgtgcag tgcgtcgtga
     2101 agtgcccggt cgtacggcag gcagaactcc cggtgcaggt ctggggaaat gacggtggaa
     2161 gaggcggcgc cgcccccggt ttcaatgagg tcgaatctgg cgcccttcag tcctgcgatg
     2221 aactgaagct tcttctcgag gagcgatcgg aggagacgat gtacccagtc gggtttgtcg
     2281 aaacaggcga gtatgagcct gttcacgtcc tccagacaac atgcatgctg ccagcacccg
     2341 gcctgatccc cccacacaaa tccccggatg atgcccctgt ctccgatttc ctcatacagt
     2401 tgcgctaccg gcgccgggtc gaacaccgca accggcatgt acttctcaat gagttcgatg
     2461 tcttcatcgt gctcgatgag gtatttggtg atccaggtgg ttttccggtc cccttccgtt
     2521 tcgtacgtca atatgccttc gggagtggtg atcacgtgcc gggctttgcg gtggtccggg
     2581 tcgttgctca gaacctgaac atcgtctctc cattgcggcg ttgaggtttt ggaaaagtcg
     2641 gcatccacga gccagaactg ccccatggac tggaaatact ggatctgcgc atccatgcct
     2701 gtcgccgcaa acgcttcgat gtccgtaatc ccgcccatat acgtctggag atggtacccc
     2761 tgccactggt gcaccgacac ggggagacgg tcgggttttt cccggtgcag tgcccgcaga
     2821 atacgttctt tgcttgtcac gtttccatcc tccacggggt gcagagaacg ccggaacaca
     2881 gaggctcagg tgtgcaacgg cacaatcgga aagtacggcg gcggatcgtc ggggcaaaat
     2941 gacgcgatcg gtcaagcgcc aaacagaaca ggcctcggag agactcctgt catggcgcgg
     3001 tgaaacgcca catcgtgctg atggaattga tctgatccgg cccctttgtg gtcgcggcaa
     3061 ggcgccagta atacgtccgc ccgctgataa ggggagttcc ggcatagatg atactcgtcg
     3121 ccgggctgtt tgcataagcc gttccatgct gcgtaaaccc acccgcgtcg gcatagatga
     3181 tcagcttgta ccccactgcc tggggcaccg caacccagcg gaattcgggt gtcagcgaag
     3241 cggttgtcgc gttgttgccc ggagccgcca gcgtggcagg aggaagtgct acatccgaca
     3301 cttcggcgct ctggctcccg ggggacttca ggccgccttt gtccaccgcg atcacccgat
     3361 agtagtaccg cgttccgacc gctacggtgg tgtcgttgaa cgacgtcgtt cctctcggca
     3421 cgatcgccac cggtgttccg acgtacggtg caggtgtagt gaccgaacgg aacacccagt
     3481 attcggcgaa gtcggcttca gggttagccg accagcttac ggaaatactg gcgggcggca
     3541 cgagattctg tcctacggcg tacacatttg cgggcaccga cggcgcggtc agattcgcgg
     3601 caatcgcgag aattggtagt gttgcagggc tctcatttcc ggcgttgtca aatgccgtca
     3661 tcttgtagta gaactggatc gtgtaatcga ggttgcggtc ggtgtacgag gtgctatcac
     3721 gagagatagt agcgattagc tggtacgcgg cagcagggtc accctcggct cggtacattc
     3781 tgtaccccgc cagatccttc tcccggtttc tctgccagcc gaagcgattc tcaccgtcgc
     3841 ccccgccgat gcgcccgaac ccggcaggtg tagcaggcgc aaccagatcc gggcccgtgg
     3901 tgccacaacc gatcgcaaag agaaccggcc agatccacgc acctgacaga aatccccatc
     3961 gtaccagcat cgacgtggct ccttctcatt cagtctgcgc caatgagaac ggcgcagccg
     4021 acgttccagc ttgtacgcaa acatacgcag cggacgccgg cccgcaacac ccctgcacca
     4081 accacaaccg gcaaaccgcg tgccgcaaaa agaaaggggc ggtccgcctc caggcagccg
     4141 ccccgcatcg cttcagccag aagcgccttc agttctgcgc gggcagacct agttgttccc
     4201 gcagcgtttt cgccttccga tcagcatact gcttccaccg cggatcgcgg ctggcctgaa
     4261 cgtacgcgtc cagcgcttca cgagtgcgcc caaggcactc cagcgcttcc gcacgggttg
     4321 catgggcaga ggcgttcccc ggctgaaccg ccagcgcacg cgtagccgcc tgctcagcct
     4381 ccccacattt gttcagtttc atgtacgttt cggcaagctt gagcggcggc cgaaagtcgc
     4441 gcggacgcag gctctccgcc cgcacgagag cggattccgc agccgcgtac tgttccagat
     4501 caaacagcgt gttgccaagg ttcaccagcg cagtgaagtt cgtcggatca atctgcaccg
     4561 cctgacgca
//
LOCUS       DAOTNG010000261         4546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15568_length_4546_cov_4.611000, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000261 DAOTNG010000000
VERSION     DAOTNG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15568_length_4546_cov_4.611000"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="P5179_11410"
     CDS             complement(<1..242)
                     /locus_tag="P5179_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95867.1"
                     /translation="MRLPDMRCVEQTFRQPQIDDPASIARTAALSLAAGVVRPGDRIA
                     VPVGSRGIAHIAGIVKAVIHALRDLRAQPFIIPAMG"
     gene            complement(321..1844)
                     /locus_tag="P5179_11415"
     CDS             complement(321..1844)
                     /locus_tag="P5179_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRS95868.1"
                     /translation="MAVSVRRAVCNRLRHLGFFLAFLTVVATPSFAHASEGGGPLPPA
                     WAVLPFILLLGMIATGPLFYHHFWEKYYPHISIALGLLVAAFYVVVLGKFGSIYHSFV
                     EYVSFIALLGSLFVASGAILIDIDKKGTPLLNVVFLFIGSVIANVIGTTGASMLMIRP
                     FMRLNRGRIKAYHVIFFIFTVSNVGGALTPIGDPPLFMGFLRGIPFFWIITHIWYIWL
                     VTLGLILAVFFAIDTINARKEAATSVEHSGTIQFTGLKSLVWLAIIIGSIFLDPNVIP
                     GFPDLKHYGIPLGIREFIMFGICYLAYKTADKKALEGNDFNFHPILEVAFLFVGIFLT
                     MVPALELIAHEAKVYGDQLTPGLFYFGTGSLSAVLDNTPTYLNFLTAALGKYSMDATN
                     PSHIRMFIDCPETLKYVVAISAGAVFFGAMTYIGNGPNFMVKSISDQNGLKMPSFFGY
                     VVKYAVPILLPIFILVWALFFIMPPGDYALIKADCPKIYEMWKGVAPVISDTLSTMH"
     gene            complement(1893..2876)
                     /locus_tag="P5179_11420"
     CDS             complement(1893..2876)
                     /locus_tag="P5179_11420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS95869.1"
                     /translation="MNASGPRHMRWLLCGFVLAALPGISTAQSLMQRAILQGQAAARD
                     SAQAHYLNAARLAAEGKYGPAIETMRKAIEFLPSEPRLRSDLASLILASGDKAGAVRE
                     LEALASQVPPYAPAVVQLGDLRLADGDTAGAVRAYQSVVGGVLPYALAYVRLGDVAQD
                     QGKRAEAVRMYQQATSIDSTLIEAWLSLGSIFVIMDRYQDALNAFDRAVRLAPDDEVP
                     NGLRALAQQRKQDYDEGIASGKMRARMILAPTREQAEQHRRQLLAGADFIALAHRLSS
                     DPTSEVGGDLGFFGEGDLIPEFEQAVKALRPGQISPVIQLPSGYAVILRVN"
     gene            3034..4119
                     /locus_tag="P5179_11425"
     CDS             3034..4119
                     /locus_tag="P5179_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95870.1"
                     /translation="MKRYFHTLTSAFTSRMRGIDMVFRCGIVGIGSWYSQAFARAVQA
                     TSRHAKLIAAAHLGQSSESVKRYTGYTREGFADEFALNLYESARDMIHHENLDVVFVC
                     AHDAEKRKYAELALREGCHVYVAKPLCLSTVDAQALREIGRETGRTISTLEPGRYDGA
                     IREARTRVVNGEIGEVISARCWIQHGQPGKMDLSESVEMSPRSGGTLFTLGVYAAGLL
                     NWLLRARPSQAWAVGGNFATPWYPHPDQVKATVVYADGRLGSCDIYFGTPCRAPAWEI
                     EAVGTQGIIRVNQDVFEGTLYASDSRIVPFYRNQNDVIFEAIRSFFESLSAGAPPDLD
                     MPEAIEIIRLCEGWKQSIETGAPVQLN"
     gene            4149..>4546
                     /locus_tag="P5179_11430"
     CDS             4149..>4546
                     /locus_tag="P5179_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRS95871.1"
                     /translation="MRAVRMVAPRKMEIVEIEVPEISQGQVLVRLLRNAICGSDLITF
                     LETQPRRYPGELGSPCHECAGVVEKSFLDGFEPGDRVLYFPPPVENGLREYVAADAHQ
                     LLKLPAEGNLSELVLAQLLGTVLRTTREIGS"
BASE COUNT         1029 a         1251 c         1377 g          889 t
ORIGIN      
        1 ctgcccatcg cgggtatgat gaagggctgc gcgcgcaagt cgcgaagcgc gtggatcacc
       61 gccttgacga tccctgcgat atgcgcaatg cctctgctgc ccactggaac tgcgatccga
      121 tcgcccggtc tgacgacgcc ggcggcaaga gacagagccg cggttctcgc gatggacgct
      181 ggatcgtcga tctgtggctg gcggaacgtc tgttcaacgc accgcatatc cggcaatcgc
      241 accggagccc cctacaagga aagcggcctg accgcgcgga gctgccaggc cgtgggagaa
      301 ctgcaacctt tcgaggcgta ttaatgcatc gtggaaaggg tatccgatat gacgggtgcc
      361 acgcccttcc acatctcgta tatcttcggg cagtcggctt tgatcagcgc gtaatccccg
      421 ggtggcatta tgaaaaacaa tgcccacaca agaatgaaaa tcgggaggag gatcggcacc
      481 gcgtatttga cgacgtatcc aaagaaactc ggcatcttca gcccgttctg atcgctgatt
      541 gatttcacca tgaaattcgg gccgtttccg atgtacgtca tggctccgaa gaacaccgcg
      601 cccgcagaaa tcgccacgac gtatttcagt gtctccgggc agtcgataaa catccgaatg
      661 tggctcgggt tcgtcgcgtc catgctgtac tttccgagcg cggcagtcag gaaattgaga
      721 tacgtcggcg tgttgtcaag aaccgcgctg agggatcccg ttccgaaata gaacaggccc
      781 ggcgtgagct ggtcgccgta caccttggcc tcgtgcgcga tcagttccag cgccggaacc
      841 attgtaagga agataccgac gaacagaaat gccacttcca gaatgggatg gaaattgaaa
      901 tcattccctt caagggcctt cttgtcggcc gttttgtacg cgaggtaaca tatcccgaac
      961 atgatgaact cacggattcc cagcggaatt ccatagtgct tgagatctgg aaaaccgggg
     1021 atgacgtttg gatcaaggaa gatggaacca atgatgattg caagccacac gaggcttttg
     1081 agaccggtga actgaatcgt tccgctgtgt tccacagacg tcgcggcttc tttgcgcgca
     1141 tttatcgtgt caatcgcgaa gaaaacagcc aggatcaggc ccagcgtgac cagccagata
     1201 taccagatat gcgtgataat ccagaaaaac gggatgccgc ggaggaatcc catgaaaaga
     1261 ggcgggtcgc cgataggcgt gagggcgccc cccacattac ttacggtgaa gatgaagaag
     1321 atgacatggt aggctttgat gcgcccgcgg ttgagcctca tgaacgggcg tatcatcaac
     1381 atcgaagcac ctgtcgttcc tatcacattg gcgatcacgc ttccaatgaa caggaacaca
     1441 acgttgagga gcggcgtgcc ctttttgtca atgtcgatca gtatggcgcc cgaagcaaca
     1501 aaaagggacc cgagaagagc gatgaaggac acgtattcga cgaatgaatg gtagatagag
     1561 ccgaatttgc cgagcacgac aacgtaaaac gccgccacca gaaggcccag tgctatcgag
     1621 atgtgcgggt aatacttctc ccagaaatgg tgatagaaaa ggggccctgt cgcgatcatc
     1681 cccagaagga gaatgaacgg caagaccgcc cacgccggtg gaagagggcc acctccttcg
     1741 cttgcgtggg cgaatgaagg cgttgcaacc acggtcagaa aggccagaaa gaaacccaga
     1801 tgacgaagcc ggttgcagac cgcgcgacgc acacttacgg ccatgaatac atccctccga
     1861 gttgtcgaca tgagagccga aataagggaa agtcagttca cccgcaggat gacagcgtac
     1921 ccagacggga gttgaattac cggactgatc tgtccgggac gaagagcctt cactgcttgc
     1981 tcaaactcgg gtatgagatc cccctcgccg aagaagccga ggtccccccc aacctcagag
     2041 gtcggatcgc tcgaaaggcg gtgtgccagc gcgatgaaat ccgctcccgc gagaagctgc
     2101 cgccgatgtt gttccgcctg ctcacgggta ggtgccagaa tcatgcgggc acgcatcttg
     2161 cccgaggcga tgccttcgtc gtagtcctgt ttccgttgtt gagccagtgc gcggagcccg
     2221 tttggaacct cgtcgtcagg tgcgagtcgt acggcgcggt cgaaggcgtt gagcgcatcc
     2281 tggtaacggt ccatgatgac aaaaatggag ccgaggctca accacgcctc gatgagcgtg
     2341 gaatctatgc tggttgcctg ctggtacatc cttactgctt ccgcgcgctt cccctgatcc
     2401 tgcgcgacgt caccgagacg aacgtaggcc agcgcatacg gaagcacgcc tcctaccacg
     2461 ctctgatagg ctcgaacggc tcctgccgta tcgccgtccg ccaaccggag atctcccagt
     2521 tgaacgacgg ctggtgcgta cggcggaacc tgcgaagcaa gtgcctcgag ttcgcgcact
     2581 gctccagctt tgtctccgct ggcaagaata aggctggcaa ggtctgagcg cagtctgggt
     2641 tcgctgggca gaaattcgat ggccttccgc attgtctcga tggcaggccc gtattttcct
     2701 tctgccgcaa ggcgcgccgc gttcaggtaa tgcgcctgcg cgctgtcacg cgcggcggcc
     2761 tgtccctgca ggatcgcccg ctgcataagc gattgcgcgg tgctgatacc cggaagggcg
     2821 gccagaacaa aaccacacaa cagccagcgc atgtggcggg ggcccgaagc attcatagag
     2881 tattccccgg tacggtgcag gcggacgcag ataacgcccg caaaatggcc gacggaagcg
     2941 caagagacat aaagatacga aaagaccgaa atgcgtgcga agggggcctg aacggcgggc
     3001 cgcagccttg aatgccgctt ggatacttcc tcaatgaaac gctactttca cacactgaca
     3061 tcggctttca cctccagaat gcgaggtatc gatatggtgt tccgctgtgg cattgttggg
     3121 attggctcat ggtattccca ggcgtttgcg cgggccgttc aggcaacctc ccgccatgca
     3181 aagctgatcg cggctgcgca tctgggccag agttcggaaa gcgtgaagcg gtacacgggg
     3241 tatacccggg agggcttcgc ggacgagttc gcgctgaatc tttacgaatc cgcacgtgat
     3301 atgattcatc acgaaaacct cgatgtcgtg tttgtgtgcg cgcacgatgc ggagaaacgg
     3361 aagtacgcgg aactggcgct tcgggaagga tgccatgtgt acgttgccaa accattgtgc
     3421 ttaagcacgg ttgatgctca ggctctgcgt gaaatcggcc gggaaacggg cagaacgatc
     3481 agcacgctcg aaccgggccg ttacgacggc gcgattcggg aggcgcgcac acgggtggtg
     3541 aacggagaaa ttggtgaggt tatcagcgcg cggtgctgga tccagcacgg acaaccgggg
     3601 aaaatggatc tctctgaaag cgtggaaatg tcgccgcgct ctggcgggac gctgttcacc
     3661 ctcggcgtat acgccgcagg gctcctcaac tggctcctgc gagcgcgacc ttcacaggcg
     3721 tgggccgttg gagggaactt tgcgacaccg tggtaccctc accctgacca ggtgaaggct
     3781 accgtagtgt acgcagacgg gcggctgggt tcgtgcgaca tctactttgg aacgccttgc
     3841 cgtgccccgg catgggaaat tgaagccgtc ggaacgcagg gaatcatccg cgtcaatcaa
     3901 gacgtgttcg agggaacgct ttatgcgtcc gactcgcgga ttgttccgtt ctatcgaaac
     3961 cagaacgatg tcatattcga ggcgatacgc agctttttcg aatcgctgtc ggccggagcc
     4021 ccacctgacc tcgacatgcc cgaggcgatc gaaatcatac gtctttgtga gggatggaag
     4081 cagtcaattg agacgggcgc accggtgcaa ctgaactgat tggcaccacg gcgaggaggg
     4141 agtactacat gcgggcagtc cggatggtgg ctccgcgaaa aatggagatc gtcgaaatcg
     4201 aggtcccgga gatttcgcag ggacaggtac ttgtgaggct tctgcggaac gcgatttgtg
     4261 gaagtgactt gatcacgttt ctggaaactc aacctcggcg gtacccgggc gaacttggca
     4321 gtccgtgcca cgaatgcgcc ggtgtggtgg aaaagtcgtt tcttgacggt ttcgagcctg
     4381 gagaccgggt attgtatttc ccgccgcctg tcgagaacgg gctacgcgag tatgttgcgg
     4441 cggacgctca ccagctcctg aaactgcctg cggagggcaa tctttccgaa ctggtgctcg
     4501 cgcaattgct cggaacggtg ttgcggacta cccgtgaaat cgggtc
//
LOCUS       DAOTNG010000262         4546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15569_length_4546_cov_4.327544, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000262 DAOTNG010000000
VERSION     DAOTNG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_4546_cov_4.327544"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /gene="uvrB"
                     /locus_tag="P5179_11435"
     CDS             complement(<1..1512)
                     /gene="uvrB"
                     /locus_tag="P5179_11435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRS95872.1"
                     /translation="MPEFQLESDYTPQGDQPRAIAELTEGILRGDRFQTLLGVTGSGK
                     TYTMASVIANVQKPTLVVSHNKTLAAQLYGEFRSFFPRNAVEYFISYYDYYQPEAYIP
                     QSDTYIEKDASINEEIDRLRLKATSALFERRDVVVVASVSCIYGLGSPEEFRRQLVVL
                     ERGKRVNRDAMLRRLVDIHYSRNDLAPERGTFRARGDIVEVRPADEDYVIRVELFDDE
                     VESLSITDPLTGHVLEKRDRVAIYPAKHFVTTVPQIEAACQSIMVELTQRLDELRREN
                     KLVEAQRLESRTLFDIEMLREVGYCTGIENYSRHLSGRQPGERPAVLLDYFPEDFLMI
                     VDESHVTIPQFRGMYAGDRSRKQMLVEYGFRLPSALDNRPLFFEEFEALIPQAVFVSA
                     TPADYELEKSGGVIVEQVIRPTGLVDPEIIVKPVRGQVDDLLEEVRKRVAAGERTLVA
                     TLTKRMAEDLTSYLEEMGVRVRYMHHEVDAITRVETLRDLRLGEFDVLVGINLL"
     gene            1708..3228
                     /locus_tag="P5179_11440"
     CDS             1708..3228
                     /locus_tag="P5179_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95873.1"
                     /translation="MKQLWNMTGPVLGLAALLLLGVHAAQAEDVTAQASTSNQSISLG
                     STTIDGEQWQRLAFRREMPFGKLAIAMDIEFFVNSEGGLSSRGWQFGNFNQVMSTLYR
                     KIYYVRYGQPRDPVYVRLMGLDDVTLGYGFLMSHYRNTLDYPGVKNLGVHFELNPAGG
                     ISVQGVLNNLLDITNGGPVVGARVAKGLGPFEVGASLVYDVDQYGGLADNNDDGLPDA
                     LEPRVGADGVNYSQIRNPSDQAQLNSILVANGQPPINWARYARQDSLYLKGHRETDSY
                     GMVGADVAMKLIDRPSLALITYGQMGISLDDNDRSGRATGWGFGAPGLMAVFGPVRAR
                     IEYRHLRDEFQPEYFNSWYDHTRATVSADSGVITLKDASLNALAGQTLNGVFGDASVR
                     VPLLGEASAQYQYLHGPTNQQRLSALAGVDQGLLQMVPKLSRIEAFYAKDNIGLYGDG
                     FFQKTVDMSYGYRVGFTVAPSVEMIWSTRWLFRPVGSDPLVVEPQRQITFATVFNF"
     gene            complement(3340..4338)
                     /locus_tag="P5179_11445"
     CDS             complement(3340..4338)
                     /locus_tag="P5179_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS95874.1"
                     /translation="MSSHFDGEKFRNQVPVPNHGFSDLIRWRFSREPGEWNDSNHVAP
                     GPAPPERVGNGDLRVTFVNHSTVLIQMDSLNILTDPIWSDRAGPFSWAGPKRVRPPGI
                     RFEDLPPIDAIILSHNHYDHTDIPTLKRLFERDAPRVFVALGNKALMDKNGMPGSQEM
                     DWWQDTPITPSVRLTFVQAQHFSARGLGDRNETLWGGYVIEGEAGVVYFAGDTGWGPQ
                     FEQVRDRFGAIRLAILPIGAFRPRWFMAAVHISPEEAVRAHRLLGAQTSMAVHFGTFR
                     LGDDGQNEPVEELANARELHDVSPEAFWVLGFGEGRMVPPVEVDTLRSAEVVEQRR"
BASE COUNT          897 a         1383 c         1409 g          857 t
ORIGIN      
        1 cagcaggttg atgcccacca gcacatcgaa ttcgccaagg cgaagatcgc gaagagtttc
       61 cacccgggtg atggcgtcca cttcatggtg catgtaccgc acgcgcacgc ccatctcttc
      121 gaggtaactc gtcaggtcct ccgccatgcg tttcgtcagc gttgcaacca gagtccgttc
      181 gccggcagcg actctcttcc gcacctcttc gaggaggtcg tccacctgcc cgcgaaccgg
      241 tttcacgatg atctccgggt ccacaagccc ggtgggccgg ataacctgct ccacgattac
      301 tccgccgctc ttttccagct cgtaatcggc gggcgtggcg ctgacgaaga ccgcctgcgg
      361 aataagcgct tcgaactcct cgaagaacag cgggcgatta tccagcgccg aggggaggcg
      421 gaagccgtac tccacaagca tctgctttcg cgaacggtcg ccggcgtaca tcccgcggaa
      481 ctggggtatc gtgacgtgcg attcatccac gatcatgagg aagtcctcgg ggaagtagtc
      541 cagcagcacg gcggggcgct cgcccggttg ccgtccggag aggtgacgtg agtagttctc
      601 gatgccggtg cagtagccca cctcccggag catttcgata tcaaacaggg tgcggctttc
      661 cagccgctgg gcttcgacga gcttgttctc cctgcggagt tcgtcgagcc gctgcgtgag
      721 ttccaccatg atgctctggc aggccgcttc aatctgagga acggtggtaa cgaaatgctt
      781 cgcgggataa atcgccacgc gatcgcgctt ttccagaacg tggccggtaa gcgggtcggt
      841 gatggagagc gactccacct cgtcgtcgaa tagttcgaca cggatcacgt agtcctcatc
      901 cgcggggcga acctccacaa tgtctcccct ggcgcggaac gtgccacgct cgggagcaag
      961 gtcgttgcgg ctgtaatgaa tgtccaccag ccggcgcagc atcgcatcgc ggttgacccg
     1021 tttcccgcgt tccagcacga caagctggcg acgaaactct tccggcgacc ccaacccgta
     1081 gatgcacgac accgacgcca caacaaccac gtcccgccgc tcgaacaacg cgctggttgc
     1141 cttcaggcgc aggcggtcta tctcttcgtt gatcgatgca tctttttcga tatacgtatc
     1201 gctctgcggg atatacgctt ccggctggta atagtcgtag taactgatga aatactccac
     1261 cgcattgcgc ggaaaaaacg accggaactc cccgtagagc tgcgccgcga gggttttgtt
     1321 gtgcgaaacc accagcgtcg gcttctggac attcgcgatt accgacgcca tggtataggt
     1381 tttgccagac cctgttacac caaggagcgt ctggaagcga tcgccccgca ggatgccctc
     1441 cgtcagttcg gcgatggcgc gaggctggtc gccctgaggg gtgtagtcac tttcgagttg
     1501 aaattccggc ataggagagc tcgttcacca gcgtagaagt cacaacgcag ataccgtctt
     1561 cctagagtat acgaacggcg gtgcttcgtg gtgcccgcga aaccggggtg gttcgcaccc
     1621 gaaggaaatt gcgcgtatca ttctcggtac gacaatccat tcgcgcgaac gcattacccc
     1681 ggagcgacac aggaaagggt gtgcacgatg aaacaacttt ggaacatgac aggaccagtg
     1741 ctcgggctgg cggcgttgct gttgctgggc gtccatgcgg cgcaggccga ggacgtgact
     1801 gcccaggcga gtacctccaa tcaatccatc tccctcggct cgaccacgat tgacggcgaa
     1861 caatggcagc gccttgcgtt ccgtcgggaa atgccgttcg gaaaactcgc catcgccatg
     1921 gatatcgagt tttttgtgaa cagcgaaggc ggcctgagca gccggggatg gcagttcggc
     1981 aacttcaatc aggtgatgag cacgctgtat cgaaaaatat actacgtgcg gtacggtcaa
     2041 ccccgggacc cggtctacgt tcggctgatg gggctggacg atgtgacgct ggggtatggc
     2101 ttcctcatgt cgcactaccg gaacacgctg gattaccccg gtgtgaaaaa cctcggtgtt
     2161 cacttcgaat tgaaccccgc gggtggcatc tccgttcagg gcgttctgaa caacctgctc
     2221 gacatcacga acggcgggcc ggtcgtcggt gcccgggtgg cgaagggact ggggccgttt
     2281 gaagtcggcg cctcgctggt atacgacgtg gaccagtacg gcggcctcgc ggacaacaat
     2341 gacgacggat tgcccgatgc gctggaacca cgcgtgggtg ccgacggggt gaactacagc
     2401 cagatccgaa atccttcgga tcaggcccaa ctcaacagca tcctggtggc aaacggacag
     2461 ccgcccatca actgggcgag atacgcgcga caggatagcc tctatctcaa aggccaccgc
     2521 gaaacggaca gctacggcat ggtgggcgcc gacgtcgcca tgaaactgat tgacaggccg
     2581 agccttgcgc tcatcaccta cgggcagatg ggtatcagcc tcgacgacaa cgaccggagc
     2641 ggccgcgcca cgggctgggg attcggtgcc ccgggtctga tggccgtatt cgggccggtg
     2701 cgggcacgga tagagtaccg tcacctgcgg gatgagttcc agccggagta tttcaactcg
     2761 tggtatgacc acacccgggc cacggtgagc gcagattcag gagtgatcac gctgaaggac
     2821 gcctcgctga atgccctcgc gggccagaca ctcaatggcg tgtttggcga cgcctcggtt
     2881 cgcgtgccgc tcctcgggga agcaagtgcc cagtaccagt acctccacgg accgacaaat
     2941 caacagcggc tgtccgcgct ggcgggggtt gaccagggcc tgttgcagat ggtgccgaag
     3001 ctcagcagga ttgaggcatt ctacgcgaag gacaacatcg ggctgtacgg ggacgggttc
     3061 ttccagaaga cggttgatat gtcgtacggc taccgggtcg gcttcacggt ggcgccgagt
     3121 gtcgaaatga tctggtctac ccgctggttg ttccgtcccg tcggctcgga tccgctggtg
     3181 gtggagccgc agcggcagat cacgttcgcc acggtgttca acttctgacg caagacgggg
     3241 aattgggtgt agcgggaggt gccggggtcg gtgcctcccg tttttctgtt ccggcgcaat
     3301 ccgccgcgcg ggaatgttgc ggcgcgcagc cggacgctct cagcgccgct gttcgaccac
     3361 ttccgcactt ctcagcgtat ccacctcaac cggaggcacc atgcggcctt cgccgaagcc
     3421 gagcacccag aacgcctccg gcgaaacatc gtgcagttcg cgggcgttgg ccagctcttc
     3481 cacgggttcg ttctggccat catcccccag ccggaacgtg ccgaaatgca ccgccatgct
     3541 ggtttgcgcg ccgaggagcc ggtgagcccg cacggcttct tccggggaga tgtgcaccgc
     3601 cgccatgaac caccggggcc tgaacgcacc gatgggcagg atggccagcc ggatggcgcc
     3661 gaaacggtcg cgcacctgct cgaactgcgg cccccagccg gtatccccgg cgaagtagac
     3721 gactcccgcc tcaccttcga tcacgtaccc accccagagg gtctcattcc ggtcgcccag
     3781 cccgcgcgcg gagaagtgtt gcgcctgaac gaacgtcagc cgcacggacg gagtgatcgg
     3841 tgtatcctgc caccagtcca tctcttgaga acccggcatc ccgtttttgt ccatcagggc
     3901 cttgttgccc agtgccacaa aaacgcgagg agcgtcgcgc tcgaagagcc gtttgagtgt
     3961 cgggatatcg gtatggtcgt aatggttgtg gctgaggata attgcgtcga tgggcggcaa
     4021 atcctcgaac cggatacccg gcggccgcac tcgtttcggg cccgcccacg agaacggccc
     4081 tgcgcgatcg gaccagatgg ggtctgtcag gatgttcagg ctgtccatct ggatgaggac
     4141 ggtggaatga ttcacaaagg tgacgcgcag gtcgccgttg ccgacgcgtt ccgggggagc
     4201 gggccccggc gcaacgtggt tcgaatcgtt ccactcgccg ggctcccggc tgaatcgcca
     4261 gcgaatcaga tcgctgaacc cgtgattcgg caccggtacc tggttccgga atttctcacc
     4321 atcgaaatgg gatgacatgg ggcctcggta cgacgggccg gaaagagcgc accccgccag
     4381 aaagaaggcg aaaaggaatg gcaaaaatga aaagcggatc atgcgacacc atctgtctcg
     4441 acgtgaacaa gactgctcag tgtcgtagaa acgctcacac cgtgcagatt ggttcccgtg
     4501 cccggcgacc tttacggcgt gctgtgaatc cggtacagcg acacgg
//
LOCUS       DAOTNG010000263         4469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_15898_length_4469_cov_5.373357, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000263 DAOTNG010000000
VERSION     DAOTNG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15898_length_4469_cov_5.373357"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="P5179_11450"
     CDS             complement(<1..181)
                     /locus_tag="P5179_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95875.1"
                     /translation="MQFRNPLAVTTVLLALLLSTACTVRAERNVDSTTTDHKLLAPVR
                     AAVPPVIDGILDDEVW"
     gene            complement(589..2007)
                     /locus_tag="P5179_11455"
     CDS             complement(589..2007)
                     /locus_tag="P5179_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95876.1"
                     /translation="MPDHPNIFVIVSDTFRPDHIGINGAGFAETPELDDFLRRGVTFD
                     NALVSSFPTIPMRTDWWTGRFGHPRYGWQDLEPSTQTLPDILRRNGYTTQLIADTTHM
                     LRSKFWEHVRHFSFRRGRESDAPFCRLNEPPARVVSDRRKVRVDPGVGEGPVCAEIQP
                     HTNFTAHYEDQAHSSLLAGDVCRWIEGNWKASPWFLWVDFFDVHEPWFPPEYLWRHYQ
                     PKYNGEPMVQPNYSTAEVYSPEELANMRARYAATCTLTSKNIGRCLRVAADAGLFENT
                     IFVFLSDHGMFLGEHNLTGKSLITPDKFDVFPFHREVARHCFTIAAPGIEGNQRSGVF
                     AQAPDLLPTLLDLAGIEAPKQPVIEGVSLVPAMRGDDSPVRSTAITAWSIRLLGNNFV
                     HSRHPAITDGDWTLMLFEPPEPQPPQLYHTKTDPGHERNVFDQHRAEAERLYADFIAF
                     MKAHDANAATLERHKPECVGLG"
     gene            complement(2305..3714)
                     /locus_tag="P5179_11460"
     CDS             complement(2305..3714)
                     /locus_tag="P5179_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRS95877.1"
                     /translation="MTQKRTSRILRTLAIHAVLVAGGVVFSLPFFWLLTTSFKADREM
                     FVLPPEWIPEIPPKVEYSPYQLTWRGQTTEQFFGLGPITVQDKDLSDHPVANSVSLWR
                     EARGAGFAPLDSFESRPLRRVAYDFRQRDAVAYEALFPSPIQVETLRRVEISYKSDRS
                     FHRLRVQVFTPEGIWVSADPSVLNSETWKSAPWQFAKRREEERDVLRLKQLVAAPQHW
                     LVGKNGEERILVRATLERVSWLGAVWTKYTENYVDSLAYIPFGRFVVNTIILTALNIV
                     LQVLSCSLIAYGFSRIRWPGRNVMFGLLLATMMIPGQVVMIPQFMIWKSMGMYNTWGP
                     LFLGSLFGSGFFIFLLRQFMLTIPNDLEDAAKIDGAGYLAVWRMVMLPLIKPALAAIA
                     IFQFMGSWNDFFTPFLYITSKELMPISLGLYMFKDARGAEYGMLMAASTMMVVPIIAL
                     FFFAQRYFIQGVTLTGLKG"
     gene            complement(3809..4174)
                     /locus_tag="P5179_11465"
     CDS             complement(3809..4174)
                     /locus_tag="P5179_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRS95878.1"
                     /translation="MVALLDTNIVLYSLGGRLAEPLPVCDCRVSVITEIEVLSYPSLS
                     ADEAAVAREFLQCVGVEALSSEVKDATIELRRKHKLRVPDAIVVATARILDAVLYTND
                     SRLLTIPEITSVPLRLRDL"
     gene            complement(4174..4395)
                     /locus_tag="P5179_11470"
     CDS             complement(4174..4395)
                     /locus_tag="P5179_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95879.1"
                     /translation="METIRYKVKIPRDRTLTVRVPHTVPVGDAEVLLVISSGRRKRPR
                     RPVDLARFNGSITLKEDPLAYQTRTREEW"
BASE COUNT          861 a         1390 c         1368 g          850 t
ORIGIN      
        1 cccagacctc gtcgtcaagg atgccgtcaa tgacgggcgg cacggcggcg cggaccgggg
       61 ccagcagctt gtggtcggtt gtggtggaat ccacgttgcg ttcggcgcgg acggtacacg
      121 ccgtggaaag aagcagcgca agcaataccg tagttacggc caaaggattt cggaattgca
      181 tgaaaatcgg ccccgagagt cagtgcttct gaatgtaacc ggggtgtgca acgcggggcc
      241 aagcgggcgc gcccgggaag cgcgccttgg cagaccggcc gttttgaggc cggcaggcat
      301 gaagtgtctt gggacagccg cgacagcaac ggccgcgcgg ttgccagcgg ggtgtatctg
      361 gcgcggctga cggcgtcgca gggcgtggtg acgagaaaga tgacgttgtt aaggtagggt
      421 gcgtgcaggc aggggcgacg catgcgtcgc ccctacggtg tgcggtccgg tgtatcgaga
      481 ccaccgcggc ggcttcgggc ggctggcact cggagacccg cgcgctgtac ggtcgtcgag
      541 tggcgggccg cccgccgagg cggctcgttg tatcgagacg acctctccct accccagccc
      601 cacgcactcc ggtttgtggc gctccagcgt ggccgcgttg gcgtcgtgtg ccttcatgaa
      661 tgcgatgaag tcggcataca ggcgttccgc ctccgcgcgg tgctggtcga acacgttccg
      721 ctcgtggccg gggtccgttt tcgtgtggta gagctgcggc ggttgcggct ccggcggctc
      781 gaacagcatc agcgtccagt ccccgtcggt gatcgccggg tggcggctgt gcacgaaatt
      841 gttcccgagc agcctgattg accacgcggt gatggcggtg gagcgcaccg gcgagtcatc
      901 tccccgcatc gccggcacga ggcttacgcc ctcgatcacc ggctgtttcg gcgcctcgat
      961 tcccgccaga tccagcaacg tcggcagcag gtccggcgcc tgcgcgaaca ccccgctccg
     1021 ctggttgcct tcgatccccg gcgcggcgat ggtgaagcag tgccgcgcga cttcccggtg
     1081 gaacggaaac acgtcgaact tatccggtgt gatgagcgac ttgcccgtca ggttgtgctc
     1141 cccgaggaac atgccgtggt cggagaggaa gacgaaaatc gtgttctcga acagccctgc
     1201 atcagccgca acgcgcaggc acctgccgat gttcttgctc gtcagtgtgc aggtcgccgc
     1261 gtagcgcgcg cgcatgttgg ccagctcttc gggggaatac acctccgccg tcgagtagtt
     1321 cggctgcacc atgggctcgc cgttgtactt cggctgataa tgccgccaga ggtattccgg
     1381 tgggaaccac ggctcgtgca cgtcaaagaa atccacccac aggaaccacg gactggcttt
     1441 ccagttgcct tcgatccacc ggcagacatc gcccgccagc agcgacgagt gcgcctgatc
     1501 ctcgtagtgc gcggtgaagt tcgtgtgcgg ttgaatctcc gcacatacgg gaccttcgcc
     1561 cacgccgggg tccacgcgca ccttgcgacg gtcggatacg acacgcgccg gaggttcgtt
     1621 caaccggcag aacggcgcgt cggattcgcg cccgcgccgg aaggagaagt ggcgcacgtg
     1681 ctcccagaat ttcgagcgca gcatgtgcgt cgtgtcggca atgagctggg ttgtgtagcc
     1741 atttctgcgc agaatgtccg gcaacgtctg cgtggagggc tcaaggtcct gccagccgta
     1801 ccgcgggtgc ccgaaccgcc cggtccacca gtcggtgcgc atgggaatgg tggggaagct
     1861 tgacaccagc gcgttgtcga acgtcacccc gcggcggagg aagtcgtcca gctccggcgt
     1921 ctccgcgaac ccggcaccgt tgatgccgat gtggtcggga cggaatgtgt ccgagacgat
     1981 aacgaagatg ttcgggtggt caggcatggt atctcccctc ggtggaaaga acaatcgagg
     2041 tgctggtttg cagcgcgttc tggcgcgtat aatgaatccc catggcgatg cagttgccgc
     2101 atccggtcca cattccggcc cgcgatcgct ggattcccgc ctgcgcggga atgacgagtc
     2161 gctcgatacc ctcggggcta cacgggctac actgcgtatg ccctccccgt cttcgcgaac
     2221 tcctcacagg tagatgccct gcgcggaagg gtagtgccgc agcaccgtgt ctcggatgat
     2281 cttttccgat tccactccca accctcaccc tttgagcccg gtcagcgtca cgccctggat
     2341 gaagtagcgc tgcgcaaaga agaacagcgc gatgatcgga accaccatca tcgtggaggc
     2401 ggccatcaac atgccgtatt cggcgccccg ggcatctttg aacatgtaca ggcccaggct
     2461 gatcggcatc agctctttcg acgtgatata gaggaacggc gtgaagaagt cgttccacga
     2521 gcccatgaac tggaagatcg caatcgccgc cagcgccggc tttatgagcg ggagcatcac
     2581 catccgccac acagccaaat agccggctcc gtcgatcttc gccgcatctt cgaggtcgtt
     2641 cgggattgtc agcatgaact ggcgcagaag gaatatgaag aaccccgaac cgaacaacga
     2701 tccgagaaag agcggccccc aggtgttgta catccccatg ctcttccaga tcatgaactg
     2761 agggatcatc accacctgcc ccggaatcat catcgtggcg agcaggagac cgaacatcac
     2821 atttcgcccc ggccaccgga tgcggctgaa cccgtacgcg atgagcgagc agctcaggac
     2881 ctggagaacg atgttgagcg ccgtgaggat gatcgtgttc accacgaacc gcccgaacgg
     2941 gatgtacgcc agcgaatcca cgtagttttc cgtgtatttc gtccagaccg cgccgagcca
     3001 cgatacccgc tccaacgtcg cgcgcacaag aattcgctcc tccccgttct tcccgaccaa
     3061 ccagtgctgc ggagcggcaa ccagttgctt cagacgcagc acatcccgtt cctcttccct
     3121 ccgttttgcg aattgccacg gcgcactttt ccaggtctcg gaattcagca cggaagggtc
     3181 ggcggaaacc cagataccct ccggggtgaa cacctgcacg cgcagccggt ggaacgatcg
     3241 gtctgatttg taactgattt ccacgcggcg cagggtttcc acctgaatgg gcgacggaaa
     3301 aagcgcttcg tacgccactg cgtcccgctg gcgaaagtcg tacgcaacac gccgcaacgg
     3361 gcgcgattcg aaggaatcga gcggcgcgaa accggcacca cgggcttccc tccacaggct
     3421 caccgagttg gcgaccgggt ggtcggaaag gtccttgtcc tgcacggtaa tcggaccgag
     3481 cccgaagaac tgctcggtcg tctgtccgcg ccaggtgagc tggtacgggg aatactcgac
     3541 cttcggcggg atctcaggaa tccattccgg cgggagtacg aacatctccc tgtcggcttt
     3601 gaagcttgtc gtcagaagcc agaaaaacgg caggctgaac accaccccgc cggcgacaag
     3661 caccgcgtgg attgcgagcg ttcggagtat tcgagatgtg cgtttttggg tcatccctct
     3721 gtgtttcccg atagtacacg ctgcttcatt ccgtgctcgg tcggtagacc gtcggacatg
     3781 ccgcggtgat gtcaccccga ctcacgtctc aaaggtcccg cagccgcaaa ggaacgcttg
     3841 tgatctctgg aatagtcaga agcctcgagt catttgtgta gagcactgca tccagaatcc
     3901 gtgcagtagc aacgacgatc gcgtccggaa ccctgagttt gtgctttctc cgaagctcga
     3961 tggttgcgtc cttcacctcg cttgacaacg cctcaactcc aacacattgg agaaattctc
     4021 gagctacagc cgcctcgtcc gccgacaaag aggggtaaga aagaacctct atttccgtaa
     4081 tcaccgacac gcggcaatca cacactggca acggttccgc aaggcggcct cccaaggagt
     4141 aaagaacaat gttggtgtcc aatagcgcta ccatcaccat tcctcacgcg ttcgcgtctg
     4201 gtaggcaagg ggatcctctt tcagagttat ggaaccgttg aaccgcgcga ggtcaaccgg
     4261 cctgcgcggc cttttgcgcc gtccagaaga gatgaccagg agcacctccg cgtctccaac
     4321 aggtacggtg tgaggcacac gcaccgtgag agtcctgtct cgcgggattt tcactttgta
     4381 gcgtattgtt tccatacggc acctcccgtc cttttcctac ttccctccgc cctcataatg
     4441 tacccacctc ggcgcgagct tgagttgca
//
LOCUS       DAOTNG010000264         4427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16077_length_4427_cov_4.643184, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000264 DAOTNG010000000
VERSION     DAOTNG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16077_length_4427_cov_4.643184"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1111)
                     /locus_tag="P5179_11475"
     CDS             complement(194..1111)
                     /locus_tag="P5179_11475"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRS95880.1"
                     /translation="MTIRTRELVLISVAAGLSVALSFLRLFSMPQGGSVTLEAIPLLY
                     VAFRFGTGAAMAAGAVSGIVQLLLRPIIVHPVQVVLDYPLAMAACGVAGVFRMVNSNR
                     SVRVGLTVFLGVVVVFVAGLQWFELRRVSRTDEIEVQRSPDSRIVMWASADTILGGLQ
                     TKIVTYRNSAAGRSSVVGVTTAHGETARDWLDYGLSVVRAKQYEWLGNFLLLAAALAG
                     IALVAARLPLTSVALGVSLASFLKWGIHVVSGAVFFSAYAPLGESAWIYSAAYNAAYA
                     IPQLLLALLLLPPLLRRGANNAGTADPGN"
     gene            complement(1092..2411)
                     /locus_tag="P5179_11480"
     CDS             complement(1092..2411)
                     /locus_tag="P5179_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRS95881.1"
                     /translation="MDDSFSIWFLIGYAFLFGLSWVFGRAERFLSLRSVASVQALVDA
                     NRLQVPQFLLKSPAKRRAAISFGIGVLAADLILIAGVFVFLASVPGLSGAGLILAYAI
                     AALLGFVPLVVTHFVVPNLKTADDMSGPLLRFAAPLLVAWAVLTFPLSLFLDKILTHL
                     AHQPENREAREEALLALVESDEVDGVLEADKRDMISSIMSIDETLVREVMVPRVDMVA
                     IERSQSLNELLDLIRETQHSRIPVYDGKLDNIVGIVHAKDVLDAVSSSGGANGSTATA
                     EDAMRESHVLFVPMTKRVDDLLRELRKQKKHMAIVVDEYGGTAGLVTMEDVLEEIVGE
                     IQDEYDEEEQPYKWLDQKKLEVSAGMSIADLAQLLGAEFPEEQGYETVAGFLYHQFGA
                     VPSKGESTRYGPYTFTVTGVDAQRITTVDIEIHGDDARGTEFDDSDA"
     gene            complement(2454..2813)
                     /gene="ybeY"
                     /locus_tag="P5179_11485"
     CDS             complement(2454..2813)
                     /gene="ybeY"
                     /locus_tag="P5179_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRS95882.1"
                     /translation="MAGEVWKRERGDVPASVAIVFVGPERMRELNRSFRGKDAVTDVL
                     TFDLADSADRVEGEIYVCPEYAAGQLRTYSTGLNQEIVRLVLHGVLHLAGHDHHTLAD
                     GKRMADHTRRYLRELAW"
     gene            complement(2997..>4427)
                     /locus_tag="P5179_11490"
     CDS             complement(2997..>4427)
                     /locus_tag="P5179_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRS95883.1"
                     /translation="VGSLVSAQRARLRQAYGWQPFLEVAGQLGIVLLVMVGVAVYLRT
                     FHPPIWTRPAWLFLCILLAWIPLLAASFVAQNPFLPVYIVPLALTAMLGTLLFGVEVG
                     LVLVVGASLLGGAMLGFEYRVVIVNVVAGFVAAFSVRNVRNRNQFLSAMLWLPLAIAF
                     TTVAIDLIQDTTAKAMWKDVWPGAVNGFTVPILAMGLLAVCEKVFSITTNMTLLELSD
                     LNAPLLRELSLRAPGSYTHSIIIANLTESAAEAIGANALLARVGAYYHDIGKMLRPDH
                     FVENQLTVRNPHDRLTPHMSALVVSAHIKDGVDLAERVGLPRQIIDFIPQHHGTMLMT
                     FFYRKAQRMYGEENVSEEAFRYHGPKPQTREAAILMMADAVEAAVRSLRERTPAHVQN
                     TVHQIIRERLDDGQFDECALTLRDLDLIEKSFLPVLAGALHKRIEYPEPITPEPLVKE
                     SQEEAATGHTHGESSEHHVNSGNRGI"
BASE COUNT          998 a         1374 c         1237 g          818 t
ORIGIN      
        1 cctgttcggg gtacgcgccg aggctttcgt gacgttccgt tccgagcacc acgaggacga
       61 cacggagaat tcgaggaatc tgttcatcac cgtggacatt tcccggacca tgaacacccg
      121 tcgatgaagt gcaaagagcc ggttgagggg gaatggagca ttttggcgca cggcaggtcg
      181 ggtaccgctc gaatcagttc cctggatctg ctgtcccggc gttgttcgcg cccctgcgga
      241 ggagcggagg aaggagtaaa agcgccagaa gaagctgcgg aatcgcgtat gccgcgttgt
      301 aggcagcgct gtaaatccac gcactctccc ccagaggagc atacgcggag aagaagacgg
      361 cgccggagac tacgtgtatg ccccacttga ggaagctcgc caggctcacg ccgagagcca
      421 cggaggtgag tggaaggcgt gccgcaacca gcgcaattcc cgccaacgcc gccgcgagca
      481 gaaggaaatt gcccaaccac tcgtactgtt tggcgcggac aacactcagc ccgtaatcca
      541 accagtctcg cgcagtctcc ccgtgagcgg tggttacccc caccacagag cttcgtcccg
      601 cggcgctgtt ccggtacgta acgattttcg tctgcagccc tccgagaatc gtgtcagcgc
      661 tcgcccacat cacgatgcgc gaatctggag atcgctgcac ctcgatctcg tccgtgcggc
      721 ttacccggcg caactcgaac cattggagcc ccgccacaaa tacaaccacc acacccagaa
      781 aaacggtaag gccaacgcga accgaacgat tggaattgac cattcggaat accccggcca
      841 cgccgcaggc agccatcgca agcgggtagt ccagaacaac ctgcactggg tggacaataa
      901 tcgggcgcag aagcaattgc actatgccgg aaacggcccc ggcagccatc gcagccccag
      961 tcccaaaccg gaatgcgacg tacagaagcg ggatcgcttc cagggtaact gatccgccct
     1021 gtggcatgga aaaaagccgc aggaaactca gagcaacgct cagccccgcc gcaaccgaaa
     1081 tgagaacgag ttcacgcgtc cgaatcgtca aattccgttc cccgtgcatc gtcaccgtga
     1141 atctcaatat caaccgtggt aatccgctgg gcgtcaacac ccgtcacggt aaacgtgtag
     1201 ggaccgtaac gggtgctttc cccctttgac gggactgctc caaactggtg atagagaaaa
     1261 cccgcaactg tctcgtaacc ctgctcttcc gggaactcgg ccccgaggag ctgcgccaga
     1321 tcggcaatac tcatcccggc cgatacctca agtttctttt gatccagcca cttgtacggt
     1381 tgctcttctt catcgtattc gtcctggatt tcaccgacaa tttcctccag aacgtcctcc
     1441 atggtgacca gccccgcggt acctccgtat tcgtccacga cgatcgccat gtgcttcttc
     1501 tgcttccgca gctccctcag caagtcgtca acccgtttcg tcatggggac gaacagcacg
     1561 tgtgattccc gcatcgcgtc ttccgcggtg gctgtactcc cgtttgcgcc accggaagaa
     1621 ctgacggcgt cgaggacgtc tttcgcgtgg acgatgccga cgatgttgtc gagtttgccg
     1681 tcgtacactg gaatacggga atgctgggtc tcgcggatca agtccaggag ctcgttgagc
     1741 gattggcttc gttcaatagc caccatgtca acgcgcggaa ccatcacttc tcgaacaagc
     1801 gtttcatcga tactcatgat tgagctgatc atgtcacgct tgtcagcctc cagcacgccg
     1861 tctacctcgt cgctctccac aagcgccaga agtgcttcct cacgcgcctc acggttttcg
     1921 ggctgatgcg cgagatgcgt cagaatcttg tccagaaaca atgaaagtgg gaacgtgagc
     1981 accgcccacg cgacaaggag cggcgcggca aaccggagga gaggcccgga catgtcatcg
     2041 gctgtcttaa ggttcggaac cacaaagtga gtgacgacca gcggaacaaa tccaaggaga
     2101 gccgcgatag cgtacgcgag aatcagaccg gcgccggaaa gccccggaac gctcgccaga
     2161 aacacaaaaa cgcccgcgat aaggatgaga tctgccgcga ggacaccgat cccgaaagag
     2221 attgcagccc ggcgtttcgc cggggactta agcagaaact gcggaacctg cagcctgttc
     2281 gcatcaacaa gcgcctgaac gctggccaca gaccgcagcg aaagaaaacg ttccgcccga
     2341 ccgaacaccc aactgaggcc gaacaggaac gcatacccta tgagaaacca gatactgaat
     2401 gagtcatcca cgacggtaaa actgttcctc cctgtaagtc ggcaatagac gcattaccac
     2461 gcaagttcac gcagatagcg gcgcgtatga tctgccatac gcttcccatc agccagtgtg
     2521 tggtgatcgt gccccgccag atgcagcaca ccgtgtaaga cgagtctgac aatctcctgg
     2581 ttgaggcctg tgctgtaggt acgaagttgc ccggccgcat actccggaca cacgtagatc
     2641 tcgccttcca cgcgatccgc ggagtccgcc agatcgaacg tcaggacgtc cgtcaccgcg
     2701 tccttgcccc ggaaactcct gttcaactcg cgcatgcgct ccggcccgac aaaaacaatg
     2761 gcgaccgatg cgggtacgtc gccgcgttct cgtttccaca cctcacccgc cattttgcct
     2821 aagccggcct tccacccccg ttgcatcccg cggaggtgag gcttcgccgg cgggtcaacg
     2881 cgcaatctgg cacgcacttg tgggtggtgc gaggcacaca taagtctgct cggacgggat
     2941 tgtgagaacg gatgtcggta tcaaaaagat acggaaaaag aagggtgagt tgggcttcag
     3001 atccccctgt tgcccgagtt gacgtggtgt tccgaagatt ccccgtgcgt gtggccggtt
     3061 gcagcctctt cctggctttc tttcaccaga ggctccgggg tgatcggttc cgggtattcg
     3121 atgcgcttgt gcagcgcgcc cgccagaacg ggcaggaaac tcttttcgat gagatcgaga
     3181 tcccgcagcg tgagcgcgca ttcgtcgaat tggccgtcat ccagacgctc acgaatgatc
     3241 tggtggaccg tattctgaac atgggctggc gtccgctccc gaagcgacct caccgccgcc
     3301 tccaccgcgt cggccatcat cagtatcgcg gcttctctcg tctgcggttt gggcccatga
     3361 tagcgaaagg cttcctcact cacgttttcc tcgccgtaca tgcgctgcgc cttgcgatag
     3421 aagaacgtca tcaacatcgt gccgtggtgt tggggaatga aatcgatgat ctgcctcggc
     3481 agccccactc tttctgcgag atcaacgccg tccttgatgt gggcgctgac cacgagagca
     3541 ctcatgtgcg gagtcaggcg gtcatgtgga ttccgcacgg taagctggtt ctcgacaaaa
     3601 tggtccggcc gcaacatttt tccgatgtcg tggtagtacg ccccgacacg cgccagaagc
     3661 gcgttagctc cgatcgcctc cgcagcactt tcggtgagat tggcaatgat gatgctatgg
     3721 gtgtagctcc ccggcgcacg gagagaaagt tcgcgaagca gcggggcgtt gaggtcagag
     3781 agctcgagaa gcgtcatgtt ggtggtgatg gaaaacacct tttcacacac cgcaagcaac
     3841 cccattgcga gaatcggaac ggtgaacccg ttgaccgcgc cgggccagac atccttccac
     3901 atcgctttgg cggtggtgtc ttggatcaaa tcgatcgcaa cggtcgtgaa cgcgattgcg
     3961 agcggcaacc agagcatcgc gctgagaaac tggtttcgat tgcgcacgtt cctcaccgag
     4021 aaggccgcca cgaaacccgc aacaacgttg acaatcacca cgcggtactc aaacccgagc
     4081 atcgcccctc ccagaaggga tgcccccact accagcacaa ggccgacctc aacaccgaag
     4141 agcagtgtgc ccagcatggc cgtgagagca agcgggacga tgtacacggg cagaaacggg
     4201 ttctgtgcaa cgaaagaagc tgcaagcaac ggtatccacg cgagcaggat gcagagaaac
     4261 agccacgccg gtcgcgtcca gatgggcggg tgaaacgttc gaaggtacac cgcaactccc
     4321 accatcacga gcaacacgat gcccagctgc cccgccactt ccaggaatgg ttgccagccg
     4381 tacgcctgcc ggaggcgtgc cctctgcgct gaaaccaggg aacctac
//
LOCUS       DAOTNG010000265         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16186_length_4402_cov_4.751553, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000265 DAOTNG010000000
VERSION     DAOTNG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16186_length_4402_cov_4.751553"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2564
                     /locus_tag="P5179_11495"
     CDS             96..2564
                     /locus_tag="P5179_11495"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS95884.1"
                     /translation="MSVARILEDLRKPAPFVRPMPFWFWNGKLDADLFIKQLDDMAEK
                     GIGDVFLHPMPQEFRPKDFVSGIEGEYLGREFMAAVRAVVEAAAQRGMRFWLYDEGGW
                     PSGVNLGRVVAARPDLKGKVARYNEEGNVEFLERGYPVDLLDPETTQTFISQVHEEYA
                     KVVGEFFGTTIPGIFTDEPRYGGRVGGAEIPWTPKLPEIFKEKTGYDISLALPILFGL
                     KVARSLPENKRAQVQADFYRVITELWRDSYFKPLHDWCAAHNLLLVGHLSGEDDLPSQ
                     LLFCGNFFTEMEYFDWPGIDVIWRQIFPGNRGNHFPKYASSAAHTRGKRWSLSESFAV
                     YGWDLTYAQMKWITDYQYVRGINTLVPMALYSDTRGARKIGTMSDQFQANPLWEHYRP
                     YAEYVGRLGGLLSAGKPVVEVGLYYPIVSLWAGEDARSVEAKVTRIATSLLDQQIDFD
                     FVDDAAIARAEISGGTMAMGDCCYSAIVVPNAPVMPVETMRRLVEFAKAGGSVTFYET
                     PPHFAARAADEAELAQLANSLSTCVASSADWDVALGMLPWTVKLAVHNANIRAIRRSC
                     EGTEIFFLTNESLTEDASLRLRLPVQGAVVAYDPDTEQTFDASPQGGFIRVTVRANGC
                     MVLLAGNTEGLTLAPPPDPLTRAQEVSGPWIATVTRQWAFRGGEIVMLSAVKGMDRDA
                     GGVLGTPKRVDTLEDWDKVYYENFCGTVDYATSLPLQAVPPRALLDLGTVGVVAEVDV
                     NGTIIGKRLWAPYTFDITSALKVGTNNIRISVTSTLFRLMTMPEIVGELKQRGWFNSY
                     AQKVSEFKGDRAPAGLIGPVRILT"
     gene            2987..>4402
                     /locus_tag="P5179_11500"
     CDS             2987..>4402
                     /locus_tag="P5179_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRS95885.1"
                     /translation="MPTRPNILFIMSDDHAAHAMSCYGSRINHTPNLDRIASEGMRLD
                     RCFCTNSICTPSRATILTGTYNHVNGVTTLDTPMDAGLQTFPKLLRESGYQTAVFGKW
                     HLGEHKSFEFCPTGFDDWAVLPGQGFYHNPEFIFKGPRGPVQRTVMGYVTDLITDFCL
                     DWLQNRDRERPFCLLYHHKAPHRHWNPDEKHAAMYLNEDIPEPDTLYDDYSNRASAAA
                     AAEMRVGVHMTQLDMKCEVPKNIPEREMRKWAYQRYIKDYLRVIASVDDNVGRVLDYL
                     DADGLTENTVVIYCSDQGFFLGDHGWYDKRFMYEESLKMPFVVRYPKEIEPGSVSDDM
                     VLNVDFAPLFLDFAGLPIPKGMQGRSFRPVLGGETPPDWRDSMYYRYWMHKAHHNVYA
                     HYGLRTKRFKLIYYYSDGLHQMGASDEKYEPEWELFDLERDPAEMNNVYADPAYAETV
                     RELKAELHRQQAAVGDARYEKD"
BASE COUNT          871 a         1394 c         1344 g          793 t
ORIGIN      
        1 ggaaggcttg acatggcttc gcattgcaag gattttgact atcaagaaca tacgtacctc
       61 tccgcgtcat attccgtcat gtaaggagcg tcggcgtgag cgtagcacga atcctggaag
      121 acctccggaa acccgccccg ttcgtgcggc cgatgccgtt ctggttttgg aacggaaaac
      181 tcgacgccga tctcttcatc aaacagcttg atgacatggc agagaaaggc atcggcgacg
      241 tattcctcca cccgatgccg caggaattcc gccccaagga cttcgtctcg ggaatcgaag
      301 gggagtacct cggcagggag ttcatggctg ccgtgcgcgc ggtggtggaa gcggccgcgc
      361 aacggggtat gcgtttctgg ctgtacgacg agggcggatg gccgtccggc gtcaacctcg
      421 ggcgcgtagt ggcggcaagg cccgacctga aggggaaggt tgcccgttac aacgaggaag
      481 gcaatgtaga gttcctcgaa cgcggttacc cggtggacct tctcgacccg gagaccacgc
      541 agacgtttat cagccaggtg cacgaggaat acgcgaaagt cgtcggcgag ttcttcggca
      601 ccaccatccc cggcattttc accgacgagc cccgttacgg cggccgcgtg ggcggagccg
      661 aaattccgtg gacaccgaag ttgccggaga tcttcaagga gaagaccggg tacgacattt
      721 cccttgccct tcccatcctg ttcgggctca aagttgcccg cagcctgccc gaaaacaagc
      781 gcgcgcaggt tcaggccgat ttctaccgcg tgatcaccga actctggcgg gatagctatt
      841 tcaagcctct tcacgattgg tgcgcggccc ataatctgct tctcgtcggc cacctttccg
      901 gggaggacga cctccccagc caactgcttt tctgcggcaa cttcttcacc gagatggaat
      961 actttgactg gcccggtatc gacgtgatct ggcgccagat attccccggc aaccggggca
     1021 accacttccc caaatacgcc agctcggccg cccacacccg cggaaagcgg tggagccttt
     1081 ccgaatcgtt tgccgtatac gggtgggatc tcacctacgc gcagatgaag tggattaccg
     1141 attaccagta tgttcgcggg attaacaccc tcgtgccgat ggcgctctac agcgataccc
     1201 gcggtgcccg gaaaatcgga accatgtccg accagttcca ggcgaatcct ctctgggagc
     1261 actaccgccc gtatgccgag tacgtaggca ggcttggcgg gctgctctcc gcgggaaaac
     1321 cggtggtcga agtcggcctg tattacccca tcgtcagcct gtgggccggc gaagacgcgc
     1381 ggtccgtgga ggccaaggtc accagaatcg ccaccagcct cctcgaccag cagatagact
     1441 tcgactttgt ggacgacgct gcgatagccc gggcggagat ttccggcgga acgatggcaa
     1501 tgggcgattg ctgctactcg gcgatcgtcg ttcccaacgc gccggtaatg ccggtggaaa
     1561 caatgcgcag gctggtggaa ttcgccaaag cgggcggatc ggtcaccttc tacgaaacac
     1621 cgccgcactt cgccgcgcgc gccgccgatg aggccgaact ggcacagctc gcgaactcgc
     1681 tctccacctg cgtcgcgtca tcagccgact gggatgttgc cctgggcatg cttccgtgga
     1741 cggtgaagct tgccgtgcac aacgccaaca tccgggcgat tcgccggtcc tgcgagggca
     1801 cggaaatctt cttcctcacc aacgaatcgc tcacggagga cgcctcgctg cgcctccgcc
     1861 tgccggtgca gggagcagtc gtggcgtacg accccgacac ggagcagacg ttcgacgctt
     1921 ccccgcaggg cggtttcatc cgcgtgacgg tccgcgcgaa cgggtgcatg gtgttgctcg
     1981 ccgggaacac ggagggcctt accctcgcgc cgccaccgga cccgctgacc cgtgcgcagg
     2041 aggtctccgg cccgtggatt gccaccgtaa ccagacagtg ggcgttccgc ggaggcgaga
     2101 ttgtcatgct ctcggcggtg aaaggtatgg atcgcgacgc cggcggcgtt ctcggcacgc
     2161 cgaaacgggt ggacacgctc gaggactggg acaaggtcta ctacgaaaac ttctgcggaa
     2221 cggtggatta tgccacctcg ctgccgctgc aggcggtgcc cccacgcgca ctgctcgatc
     2281 tcggaaccgt cggcgtagtg gcggaagtcg acgtgaacgg aacgattatc ggcaaacgcc
     2341 tgtgggcgcc ctacacgttc gatatcacct ccgcgctgaa ggtcggcaca aacaacatcc
     2401 gcatatccgt taccagcacc ctgtttcgcc tgatgaccat gccggagata gtcggtgaac
     2461 tgaaacagcg cggctggttc aacagttacg cgcagaaggt ctccgagttc aaaggtgacc
     2521 gcgcccccgc cgggttaata ggccccgtac gcatcctcac ttgaaccatg cccaggcggg
     2581 gcaccccacc gggttagtag gccccgtacg gcttcttacc gggtagccta ccgggggtgg
     2641 gacaggcatt cttgcctgtc cgttgctggc atgaaaggcg aggcgggaca tcccgcttcc
     2701 gggcccctgt gtgccgtcat tcccgcagcc tccatgccac ggggagaccc gcgttgtcgc
     2761 cgcacgatga ccggtcatcg agtggcgagc gcgtggtccc tgagcgcgct cccgtcggcg
     2821 ggagcctgtc gaagtgcagc gctcgatgta tcgagatgat cacgccaata cggccaatcg
     2881 gtgttcgatc tctccgaacc cggttggccg cgcacaccga attccagaaa tttgctactg
     2941 aatccatccg gttaccgcgg ccgtcccctt cgaggagtct gcacccatgc ctacgcgtcc
     3001 gaatatcctg ttcatcatgt cggatgacca cgccgcgcac gcaatgagct gctacggcag
     3061 ccgcatcaac cacacgccga acctcgaccg catcgccagc gaaggcatgc ggctcgaccg
     3121 ctgcttctgc accaactcca tctgcacgcc gagccgggca accatcctca ccggcacgta
     3181 caaccacgtg aatggggtca ccacgctgga tacacccatg gacgccggcc tgcagacgtt
     3241 tccaaagctg ttgcgggaga gcggttatca gacggcggtg ttcgggaaat ggcacctcgg
     3301 tgagcacaaa agctttgagt tctgccccac cggcttcgac gactgggccg tgttgccggg
     3361 gcaggggttt taccacaatc ccgaattcat tttcaagggg ccccggggac cggtgcagcg
     3421 cacagtgatg gggtacgtga cggacctgat taccgacttc tgcctcgact ggctgcagaa
     3481 ccgcgaccgc gaacggccct tctgcctgct ctaccatcac aaggcacccc accggcactg
     3541 gaaccccgac gaaaaacacg ccgcgatgta cctgaacgag gacatcccgg aacccgacac
     3601 gctctatgac gactactcca accgggcttc cgccgctgcc gccgcggaga tgcgcgtggg
     3661 cgtgcacatg acccagctcg acatgaagtg cgaagtgccg aagaacattc cggagcgcga
     3721 gatgcggaag tgggcgtacc agcgctacat caaggactac ctgcgcgtca tcgccagtgt
     3781 ggacgacaac gtcggccgcg tgctggatta cctcgacgcc gacgggctga cggagaacac
     3841 cgtggtgatc tactgctccg atcagggatt cttcctcggc gaccacggct ggtacgacaa
     3901 acggttcatg tatgaggagt cgctgaagat gccgttcgtg gtgcggtacc ccaaggagat
     3961 cgagccgggc tccgtgagcg acgacatggt gctgaacgtg gatttcgcgc cgcttttcct
     4021 cgatttcgcc ggcttgccta tccctaaggg gatgcagggg cggagcttcc ggccggtcct
     4081 gggtggtgag acgccgccag actggcgcga cagcatgtac taccgctact ggatgcacaa
     4141 ggcgcaccac aacgtttacg cgcactatgg cctgcgcacg aaacggttca agctcatcta
     4201 ctactactcg gacggcctcc accagatggg tgcgtcggat gagaagtatg agccggagtg
     4261 ggagctgttc gaccttgaac gcgatccggc ggagatgaac aacgtctacg cagacccggc
     4321 ctacgccgaa acggtgcgcg aactcaaggc ggagctccac cggcagcagg cggcggtggg
     4381 cgacgcgcgg tatgagaagg ac
//
LOCUS       DAOTNG010000266         4395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16216_length_4395_cov_4.609677, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000266 DAOTNG010000000
VERSION     DAOTNG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16216_length_4395_cov_4.609677"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="P5179_11505"
     CDS             complement(<1..1223)
                     /locus_tag="P5179_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95886.1"
                     /translation="MTDQQRYDSLGCYGFPAAHTPNLDRLAREGALFEKFYVTNPICT
                     PSRSSMFTGKHLHGHGVYTVYGNLPEDEVLFPEHLQRAGYETALFGKLHIRSRMLENS
                     ERHPHDGFDVYEWALEACLDLDSPLNGYGKWLKERDPVFFEQLKRDGRKLLHHPAHLH
                     FTHWAAERTIDYLKRQDGSRPFFAMMSIFDPHNPYEAWPEEFGRLVDAGKIPDPLVNE
                     GEMERLPRGIRQEHEGSYLGGFSRYTMDDLRRMRFGYHVSIAFADHEFGRVLDTLRER
                     GLEENTLVIFASDHGDMLGDHSLLVKGAYFFDPNIRVPLIMRWPAGFAGGVRVSSLVQ
                     IQDLAATVLAAAGLDPDDVRAKAPEAENLLPLATGEKQRVRDWAACIYRNTGIKEGGV
                     YFDPEIHATMFMDER"
     gene            1450..2352
                     /locus_tag="P5179_11510"
     CDS             1450..2352
                     /locus_tag="P5179_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95887.1"
                     /translation="MDNVLLKTSELSVVVGNNEPGSGDFAAHRAGYNGIWSLTSLHSP
                     ENCFVPFYAGLNLEHCMDDLFMTEEGVDNFEPRHAPMELKQLSGGSACLYQPLTPLTG
                     VESETVFEVKEPYVIDMRFAATLHRPPRVGRWFGFFWASYVNAPDSPALYFRDADGML
                     NAMAPDVHGEGGANTVCHASVEDPALGDPGRRYAGHSLAHSWSRRRFGAPLMFGRPGD
                     GRMLYLQMFDQQSPVRITMSPSGGGRNEEKKAFNPAWDFQYVRGEATTGTEIRLRSRI
                     VYKQYDGLEEIDRFYTEWEQHVAG"
     gene            complement(2409..2636)
                     /locus_tag="P5179_11515"
     CDS             complement(2409..2636)
                     /locus_tag="P5179_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95888.1"
                     /translation="MRLFSCLFVPILCFAGTGVAAAPTAESAVPAAVLAHNAGHVVLR
                     SWLVGGMFPSTPLAQPQGGDTRLEGHTQKAV"
     gene            2891..3475
                     /locus_tag="P5179_11520"
     CDS             2891..3475
                     /locus_tag="P5179_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95889.1"
                     /translation="MGLLSLVILLASAYATARYNVFGDVAWSEWPTLVLNKILALSAL
                     ALLAIHTIRRRGVQETSARTIVGVAGFLAAVHVLLSLVLLSPAYYGKLFNLDRLTASA
                     GIAMLLGALAAATLAVGGKLLVGPDTREPRRYFAFISFVIGLHAAFQGFFGWFTPWNW
                     PGGMPPISLLSFALGIVALVAGLRRGESPSRSNR"
     gene            3495..>4395
                     /locus_tag="P5179_11525"
     CDS             3495..>4395
                     /locus_tag="P5179_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRS95890.1"
                     /translation="MAETRNIRKVLRARPTLEGAGVHLNRVFGGEHVPQFDPFLLLDD
                     FSSNNPAEYIKGFPWHPHRGIETITYVLAGEVEHGDSMGNSGVISSGDVQWMTAGGGI
                     IHQEMPKGDAQGVLRGFQLWANLPAANKMMAPRYRGIEQAQIPQVTLPGGATARIICG
                     EAAGAKGPVGDVVIEPLYMDITVPAGGELHFPVPKGHTVMAYVIDGKGQFAPESDPFA
                     FDVEGRNYFDLERECQIGTRNLVVFGEGDELAIAGGDAGVRFLLIAGKPIGEPVAWHG
                     PIVMNTQAELRQAISDLQNGTFIR"
BASE COUNT          824 a         1263 c         1464 g          844 t
ORIGIN      
        1 tggcgctcgt ccatgaacat ggtcgcgtgg atttccggat cgaagtagac gccgccctct
       61 ttgatgccgg tgttccggta gatgcaggcg gcccagtcgc gcacccgctg cttttcgccg
      121 gttgccagcg gaaggaggtt ctcggcttcc ggcgctttgg cgcggacgtc gtccgggtcg
      181 agcccggcgg ctgccaatac ggtggcggcg aggtcctgaa tctggacgag cgaagagacg
      241 cgcactccgc cggcgaagcc cgccggccag cgcatgatga gcggcacgcg gatgttcgga
      301 tcgaagaaat aggccccctt caccagcagg ctgtggtcgc ccagcatgtc gccgtgatcg
      361 gacgcgaaga tcacaagggt gttctcttcg aggccccgct ccctcagggt gtcgagcacg
      421 cggccaaact cgtggtcggc gaatgcgatg gacacgtggt acccgaaacg catccttcgg
      481 aggtcgtcca tggtgtagcg tgagaacccg ccgaggtagc tgccctcgtg ctcctgccgg
      541 atgccgcggg gcagacgctc catctccccc tcgttgacga gcgggtcggg gattttgcct
      601 gcgtcgacaa ggcggccaaa ttcctccggc cacgcttcgt acgggttgtg cgggtcgaag
      661 atgctcatca tggcgaagaa cggtcgtgag ccatcctgcc gtttgaggta atcgatcgtc
      721 cgctcggcgg cccagtgcgt gaaatggagg tgtgccgggt gatgcaggag tttgcggccg
      781 tcccgcttca actgttcgaa gaaaaccgga tcgcgctcct tgagccattt cccgtagccg
      841 ttgagcggcg aatcgagatc gaggcaggcc tccagcgccc actcgtagac atcgaatccg
      901 tcgtgcggat ggcgctcgga gttctcgagc atccggctgc ggatgtggag tttgccgaac
      961 agcgcggtct cgtagcccgc ccgctggagg tgctcgggga acaggacctc gtcctcaggc
     1021 aggttgccgt agacggtgta cacgccgtgc ccgtgcaggt gcttaccggt gaacatgctc
     1081 gaccggctcg gggtgcagat ggggttggtc acgtagaatt tctcgaacaa cgccccttca
     1141 cgagccagac ggtcgagatt gggcgtgtgc gcagccggaa atccgtagca accgagcgag
     1201 tcgtatcgct gctggtccgt catgaggagc aggatgttcg ggcgcgacgg ggttggcatg
     1261 gcagactacc ttgtttcgaa gtgatttcgg gaggtttcgc aagcgaaggt accgacggcc
     1321 gggccgcgaa atcaacagcg ggaccgggaa tggtgtcgcc cgcgcttgcc cccgccccat
     1381 gagagcgcta tcctgcaaca tgacggaggg gagtgaacga gcgaaccgga tgcgcggaaa
     1441 ggataccaca tggacaatgt gctgctgaaa acgagcgagc tttccgtcgt cgtcggaaac
     1501 aacgaacccg gttccggcga cttcgccgct catcgcgccg gatataacgg catctggtca
     1561 ctgacgagcc tgcacagccc tgaaaactgc ttcgtgccgt tctacgcggg gctgaacctc
     1621 gagcattgca tggacgacct gttcatgacc gaggaggggg ttgacaactt cgagccgcgc
     1681 catgcaccga tggagctgaa acagctttca ggcggttctg cgtgcctcta ccagccgctc
     1741 acaccgctta cgggcgtgga atccgagacc gtgttcgagg taaaggaacc ttacgtcatc
     1801 gacatgcgct ttgccgccac cctgcaccgg ccgccacggg tggggagatg gttcggcttt
     1861 ttctgggcaa gttacgtcaa tgcgcccgac agccctgcgc tgtatttccg cgacgcggac
     1921 gggatgttga acgcaatggc tccggacgtc catggcgagg gtggagccaa cacggtctgc
     1981 catgcgtcgg tcgaagaccc tgccctcggg gaccccgggc ggaggtatgc cgggcacagc
     2041 cttgctcatt cctggagtcg gcggcggttc ggagcgccgc tcatgttcgg ccggccgggc
     2101 gacgggagga tgctgtacct ccagatgttc gatcaacaga gcccggtcag gattacgatg
     2161 tcgccttcgg gcgggggcag gaacgaggag aagaaggcct tcaatccggc ctgggatttc
     2221 cagtacgtgc ggggcgaagc caccacgggg acggagattc ggcttcgcag ccgaatcgtc
     2281 tacaagcaat atgacgggct tgaggagatc gaccggttct acacggaatg ggaacagcac
     2341 gttgccggct gactttcagg caagccacga agcacgaatt tcagagcagc gacgcatcat
     2401 gctcagattc atactgcctt ctgtgtgtgt ccttctagtc gcgtatcccc gccctgcggc
     2461 tgcgcgaggg gagtgctggg gaacatgccg ccgacgagcc acgatctgag caccacgtgg
     2521 cctgcgttgt gcgccagaac ggcggcgggt accgcacttt ccgcggtcgg cgctgcggcg
     2581 actcccgtcc cggcgaaaca gagaatgggt acgaacagac atgaaaacag gcgcatggtc
     2641 tggtgctccc tgcagtgggg gaattggaag ggatacccgt aaatatgcgg catctttctg
     2701 cgtcagtcaa ccccatccgt cgagctggga aggaacaatg ttaccggcga cctagttgta
     2761 gatgcgcgaa gtgtgggaaa tgttacacga ggattcgtgt ctattacggc ttgacatttt
     2821 cacgaggcct ccgtatcctg ctcgacgggc gtcatgaacg cagccacaat acgggaagga
     2881 aggagcgcgc atggggttgc tgtctctggt gattctactg gcaagcgcgt atgcgaccgc
     2941 acggtacaac gtgtttggtg atgttgcctg gagcgaatgg ccgaccctgg ttctgaacaa
     3001 aatccttgcg ctgtcggccc ttgcgcttct ggcaatccac accatccgcc ggcgcggcgt
     3061 tcaagaaaca tccgcgcgta ctatcgtcgg cgtcgccgga tttctggcgg ccgttcatgt
     3121 tttgctttcg ctggtgctgc tttcgccggc atactacggc aagcttttca acctcgacag
     3181 actcactgcg tccgccggaa tcgcgatgct gctgggcgcg ctcgctgcgg cgacgctggc
     3241 ggttggcgga aaactgttgg tcggaccgga tacgcgcgag cctcgcaggt acttcgcttt
     3301 catcagcttt gtgatcggac tgcatgccgc gttccaggga tttttcgggt ggttcactcc
     3361 ctggaattgg ccgggcggca tgccgcctat cagccttctt tcatttgcgc tgggaattgt
     3421 agccctcgtg gcagggctgc gcaggggtga atcgccgtct cgcagtaaca ggtaacggga
     3481 accaggaggg ccaaatggcg gaaacaagga acatcagaaa ggtgctgcgg gcgaggccga
     3541 cgctcgaggg tgcgggtgta catctgaatc gcgtgttcgg gggcgagcat gtgccgcagt
     3601 tcgatccgtt tctgctgctc gacgatttca gttcgaacaa tccggcggag tacatcaagg
     3661 gcttcccttg gcacccgcac cggggaatcg agacgatcac gtatgtgctt gcgggcgaag
     3721 tggaacacgg cgacagcatg ggcaactcag gtgtcatttc ttcgggcgac gtgcaatgga
     3781 tgaccgcggg cggcggaatc attcatcaag agatgccgaa gggcgatgca cagggcgtgc
     3841 tgaggggatt ccagctctgg gcgaacctgc ctgcagccaa caagatgatg gccccgcgct
     3901 accggggaat cgaacaggcc cagattccgc aggtgaccct gcccggcggc gcgacggccc
     3961 ggatcatctg cggcgaagcg gccggcgcga aagggcccgt gggggacgtt gtcatcgagc
     4021 cgctctacat ggatatcacc gttccggcgg gcggcgagct ccactttccg gtgcccaaag
     4081 gccatactgt gatggcctac gtgattgacg ggaaggggca gttcgcgccg gagagcgatc
     4141 cgttcgcatt cgacgtggaa ggccgcaact acttcgatct cgagcgcgaa tgccagatag
     4201 gaacgaggaa ccttgtggtg tttggcgagg gggacgaact cgcgatagca ggcggcgacg
     4261 ccggggtccg cttcctcctc attgcgggca agccgattgg tgagccggtg gcgtggcacg
     4321 ggcctatcgt gatgaacacg caggccgaac tgcgccaggc catcagcgac ctgcagaacg
     4381 ggacgtttat caggt
//
LOCUS       DAOTNG010000267         4394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16222_length_4394_cov_4.749251, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000267 DAOTNG010000000
VERSION     DAOTNG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16222_length_4394_cov_4.749251"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="P5179_11530"
     CDS             <1..981
                     /locus_tag="P5179_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS95891.1"
                     /translation="DTLGNVLGGARKIAGLDADIFHSLHHFLPFGVRGRTVLTLHDFI
                     WLDHPDLAYPNRWKGRVIQAYASLALNYALRRANHIIAISEHTARRARERMAPRIPEI
                     SVIPHGVDERFHRAAKRHASGFATTEAPFFLVVGNSKPNKNVARIVGAMALLVQKVPE
                     VRLRIVGRGEGYRELRSQVDSLGLTQKVDFFGNVPDEELLDALQRAVALVFPSLVEGF
                     GMPVLEAQAVGCPVITSDRGVLPETAGDAALFVDPYDAWDIARAMETLYRDLPLRESL
                     VRKGRERATRFTWEETAKKTRHVYDLVMGQSLPEFHAVRQVLAPAAGVQA"
     gene            982..2505
                     /locus_tag="P5179_11535"
     CDS             982..2505
                     /locus_tag="P5179_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS95892.1"
                     /translation="MSPAARTHRSDAALLARGAIVNLLGNIGRLSRAFSLMLIAWLFG
                     AEALGLYVLAWATTEFLRRFVSYGIEPTIVYKLGPLLVSHDDTRIAQTIASSLVLVGV
                     TSLVGSLALYFGAPFIANTVLREPRLVEVLPVFVFAMPAFAVTSVFLAATRAKKIMRF
                     EIIARSFVEPFVLLLGTLVAWAAKTGVTGLVVAQTSAFWISAFFCAYFYGTQFPLRAL
                     FAALRRPSHLAGVFHFATPVAAKDVIAQGVARIDLFLVGHLLDAKAVGVYGIVLELVV
                     FSKHARQALEPIMAPLVAEQHHTADRERLHRTYANATRWALLVNLGLFGVAQIAGDSV
                     LSIYGKDFKAGGTALAILFFGQIVSGTFGLSETVLLMVGRPALMLGDMALLLAMIGVF
                     DYAAIQVWGLTGAALGTAGATMLVVLLQVRQARKKAGVHPWSRALLKPVGACALGLAV
                     AHLLPLGELDGITSDVVKTATFSAIYSLLLVWLGMEAEEKHLGAWVKAKLHSLLNEG"
     gene            2737..3120
                     /locus_tag="P5179_11540"
     CDS             2737..3120
                     /locus_tag="P5179_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95893.1"
                     /translation="MRRLVWVLALALSAMVAACGGGQQEIGTTARLGGSNTRIRIVND
                     LGSYTIRYVYISPASDDSWGSDRLGASQVLRPGQSETWDIDPGTWDLRVKDEDGDTYT
                     KRGIRLSSGKTYVWTVRLSDLDRRR"
     gene            complement(3117..3749)
                     /locus_tag="P5179_11545"
     CDS             complement(3117..3749)
                     /locus_tag="P5179_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95894.1"
                     /translation="MSFGVCRPFAINELALGYAGGGYSSNRWGVSGAVDSWGWEVYRE
                     SRTTLAIGRKMGKRWSLGTSIRTKWVQIEHFRSSGVADLTLDVCANPVAGLRVYGQVT
                     GIARNNWGEALLNTPRSVLCAADWKVGLVDLRGAVTRETSNVHTTDVSASFRFYPWLS
                     FTVARASGPAAHLFGVEIGRSHAAAYSLSLHPVLPATQTIRFRESWRRPD"
     gene            complement(3944..>4394)
                     /locus_tag="P5179_11550"
     CDS             complement(3944..>4394)
                     /locus_tag="P5179_11550"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRS95895.1"
                     /translation="FAYVPRSFSQEVRPDSLSNSVQRTPAGAFKRSLLFPGWGQRYNG
                     KPWKGAVYTGIVAASAAMAAKNWDTALGTTTAGSLFWSSSAWTRGRNNWLILGAFAYA
                     LAGVDAYVDAHLNTFDVGPITAQPVVTRDRRSVLVAFRVPLSFLERQ"
BASE COUNT          839 a         1296 c         1343 g          916 t
ORIGIN      
        1 gacaccctcg gtaacgttct cgggggcgca cggaagatcg cggggctgga cgcggacatt
       61 tttcactctc tgcatcattt tctccccttc ggtgttcgtg gacgcaccgt cctcacgctt
      121 catgacttca tctggctcga tcaccctgat ctcgcctacc cgaaccgctg gaaggggcgg
      181 gtgatccagg cgtacgccag ccttgccctc aattacgcgc tgcggcgagc gaatcacatc
      241 atcgcaattt cggagcacac tgcccggagg gcgcgggagc gtatggcccc gcgaattccg
      301 gagatatcag tgatccctca cggggtggac gaacggtttc accgggcagc aaaaaggcat
      361 gcttccggct ttgccaccac ggaagctccc tttttcctgg ttgtcgggaa cagcaaaccg
      421 aacaagaacg tggcccgcat agtcggtgcg atggcgttgc tcgttcagaa ggttccggaa
      481 gtcaggctcc gcattgtcgg caggggtgaa gggtaccgcg agttgcgcag ccaggttgat
      541 tccctcggcc tcactcagaa ggtggacttc ttcggaaacg taccggacga ggagctgctt
      601 gatgccctcc agagagctgt tgcgctggtt tttccgtcgc tcgttgaagg ttttggcatg
      661 cccgttctgg aagctcaggc agtcggctgc ccggtaatca ccagcgaccg cggagtcctg
      721 ccggaaacgg cgggagacgc agccctgttc gtagacccgt atgacgcctg ggatatcgcc
      781 cgcgcgatgg agactcttta ccgggacctc cctctgcggg agtctcttgt acggaaaggt
      841 cgggaacgcg ccacccgctt cacatgggaa gagaccgcga aaaagacacg acacgtatac
      901 gaccttgtga tgggccaatc tcttcccgaa ttccacgcgg tacggcaggt tcttgcgcct
      961 gccgccggtg ttcaggcgta aatgtctccc gcagcacgca cccacaggag tgacgccgcc
     1021 cttctggcac gcggcgcaat tgtaaacctc ctcggcaaca tcggccgtct ttcccgcgca
     1081 ttctccctca tgctcatcgc ctggcttttc ggcgcggaag cactggggtt gtacgtcctc
     1141 gcctgggcta cgacggaatt tctacgccgg tttgtcagct atggcattga accgacgatc
     1201 gtgtacaagc tgggtccctt gctcgtatcg cacgacgaca cacgaattgc tcaaacgatc
     1261 gcatcgagcc tggtgctggt cggcgtaacc tcgctggttg gcagtctggc gttgtatttt
     1321 ggagcgcctt tcatcgcgaa cacggtcctg cgcgagccac ggctggttga agtcctgcct
     1381 gttttcgtct tcgccatgcc ggcgtttgcg gtcacttccg ttttcctcgc tgcgacccgc
     1441 gcgaagaaga taatgcggtt cgaaatcatc gcgagatcgt tcgttgagcc atttgttctc
     1501 ctgctgggca cgctggtggc atgggctgcc aaaacgggtg tgacagggtt ggtcgtagct
     1561 caaacctcgg cgttctggat ttcggcgttt ttctgcgcgt atttctacgg aactcagttc
     1621 ccgctgcgcg cgctgtttgc ggcgcttcgc cggccttcgc atctcgcggg ggtgttccac
     1681 tttgccactc ccgttgcggc aaaagacgtg atcgcgcagg gcgtcgcgag aattgatctg
     1741 tttcttgtcg gccatcttct cgatgcgaag gcagtgggtg tgtacggcat agttctcgag
     1801 ctcgtggtgt tttcgaaaca tgcccgacag gcgcttgagc ccatcatggc gcccctggtt
     1861 gccgaacaac accacaccgc ggaccgtgaa cgattgcacc gcacctacgc gaatgccaca
     1921 cggtgggcat tgctcgttaa tctcggactt ttcggagttg cgcagatcgc gggagactcg
     1981 gtgctctcga tctacggaaa ggacttcaaa gccggcggca ccgcactggc aatcctcttt
     2041 ttcgggcaga tcgtaagcgg gacgtttggc ctctcggaaa cagtgctcct tatggtggga
     2101 cgccctgccc tgatgctggg cgacatggcg cttcttctcg cgatgatcgg agttttcgac
     2161 tacgcggcga ttcaggtctg gggtttgaca ggtgccgccc tcggcaccgc cggtgcaacc
     2221 atgttggttg ttcttctgca agtgcgacag gcacggaaga aggcgggggt tcacccctgg
     2281 agcagggcac tgctcaagcc cgtgggcgcg tgcgcacttg gcctcgcagt tgcacatctc
     2341 ctccctctgg gggaacttga tgggatcacg tcggacgttg tgaagacagc tactttttcc
     2401 gctatctaca gtctcctgct tgtctggttg ggcatggaag cggaagagaa acatctggga
     2461 gcgtgggtaa aagcgaaact ccattccctg ctcaacgaag ggtgacgcag ggcaatcgcc
     2521 gcgatgagca ctgaaggttg tgcgcggcga aaaaaaaata caacgccaca ggccggaacc
     2581 actatccaat cgccggtgtt gccagtatct tttttgtgag agcgattgca cctgcgcccg
     2641 gaatttcact gccacgctgt ggaacctcgg tgttgcccgc ggcgccaagt tcgtaccaat
     2701 gccgatgaga tcggcgcaca gaggagaggg tccggaatgc gacgtctggt gtgggtgttg
     2761 gcgttggcac tgtctgcgat ggtagcggcc tgcgggggcg gtcagcagga aatcggcaca
     2821 accgcccgtc tcggtggcag caacacgcgg attcgaatag tcaacgatct gggaagttac
     2881 acgatcaggt acgtgtatat ttcgccggcc tcagacgaca gctggggaag cgaccgactc
     2941 ggcgcaagcc aggttctccg tcccggccag tccgaaacgt gggacattga cccggggacg
     3001 tgggatctgc gtgtgaagga cgaagacggc gacacgtaca cgaagagggg aatccgcctg
     3061 agcagcggca aaacgtacgt gtggacggtc cgcctgtcag acctcgacag acggcgttaa
     3121 tccggccggc gccagctttc cctgaaccgt atcgtctgcg ttgccggcag aaccggatgt
     3181 agagacaggg aatacgcagc cgcgtgcgag cgcccgatct cgacgccgaa caggtgcgcg
     3241 gcgggaccgg acgctcgcgc gacggtgaac gacagccacg ggtagaagcg aaatgatgcg
     3301 ctgacatcgg tggtatgaac gttactggtt tccctcgtga cggcacctct gaggtcaacg
     3361 agccctactt tccagtctgc tgcgcacaga actgaacgcg gcgtgttcag gagcgcttcg
     3421 ccccagttgt tgcgggcaat acccgttacc tgaccataca cgcgcaggcc cgcgaccggg
     3481 ttggcacaca catcaagagt gaggtctgcc acgccggacg accggaaatg ttctatttga
     3541 acccatttcg tacggattga agtgccaaga gaccaccttt tgcccatctt tctgccaatc
     3601 gccagcgttg ttcgcgattc tctgtagacc tcccagcccc agctatctac tgcccctgat
     3661 acgccccaac ggtttgagga gtaaccaccg cccgcgtatc ccagagccaa ttcgttaatc
     3721 gcaaaagggc ggcatacacc aaacgacacc atcgcttcct ggctgtttcc tggtaacgcg
     3781 gcgggcctgt tccagggaga gagaccgtct gcgaagcgca aaggcaaagc ttcaccaagg
     3841 aaggtatttt cggggacgaa tgcggcgctc gccggcgagg ggagagagaa caccgccgtc
     3901 aggaatgcaa cctgcacgat tcggaggttg ccggtcgcgg atttcactgt cgctcaagaa
     3961 aggagagtgg aacgcggaac gcgacgagga cgctccttcg gtcccgggtt accacgggtt
     4021 gagcggttat cgggccgacg tcgaacgtgt tgagatgcgc gtcaacgtat gcgtcgacgc
     4081 ccgccagcgc gtaggcaaac gcgccgagga tcagccagtt gttccgcccc cgggtccacg
     4141 cggaactgct ccagaacaga cttcccgcgg tagtggttcc gagtgcggtg tcccagtttt
     4201 tcgcggccat agcggcgctt gctgccacga tgcctgtgta caccgcccct ttccacggtt
     4261 tcccgttgta ccgttgtccc cagccaggga agaggagcga gcgtttgaat gctcccgcag
     4321 gcgttcgttg gaccgagttg ctcaggctgt cgggacgcac ttcctgcgaa aaagaacgcg
     4381 gcacgtacgc gaac
//
LOCUS       DAOTNG010000268         4353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16398_length_4353_cov_3.930200, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000268 DAOTNG010000000
VERSION     DAOTNG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16398_length_4353_cov_3.930200"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="P5179_11555"
     CDS             complement(<1..696)
                     /locus_tag="P5179_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95896.1"
                     /translation="MPRSVGVSSYALVGVVITIVCIGCAAITGRGAQRTEAPVNSEEQ
                     QIRLALEQWKQAPEQDTRGMAPRTSFNFARAAVQVTGSAAVVEPVYALTGWGLGVFSF
                     SLRKKDDQWVFESWRHVGEGVIETALPDLDPGYLPLLNRWMEANLGWVQKTLRKNPPN
                     GGDQILRRHALCMLDEPLHIRSAPALEPVKQFIRSNVDLAISQIRTERVAAGATIWKF
                     YNHGWVVRTANHTW"
     gene            complement(724..2697)
                     /locus_tag="P5179_11560"
     CDS             complement(724..2697)
                     /locus_tag="P5179_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS95897.1"
                     /translation="MGSDTRLRNDVHPAEMRAKADWITGTAIKGSGEPPFSFVYGGRV
                     SSEFLGSLRRESESRTLDAERTELTSVWRDDRTGLEVRCTATEYHDLPAVEWLLTFTN
                     TGTQDTPIISDIRALDMALKLGTKGCVLHRSLGDSNSAQSFAPVDEPLVPGAAERVFA
                     PKGGRSSDGHMPYFNMDWKTGGIVLAIGWSGQWEAGFHLTSTGDMRVRAGQQTTHFLL
                     HPGESVRCPRIVLVFWDGDDHLRGNNLFRRLAIKHYYPRRDGKPVFPPICCSVATVAA
                     DGTYEKPHLDAVGPMAERGIEVFWSDMDPQQWYPIGFPEGTGNWEIDRAKYPNGLKPI
                     GDAVRAAGMGYLLWFEPERVHPGTMVEREHPEWVMKAEGEWSQLFCMHDPVARAWLTD
                     LIDRFVTEAQLAWLRWDFNIEPLTFWQRNDTPDRVGITEIRHIEGLYAMWEELERRHP
                     GLLIDNCSSGGRRLDIEAARYGLPLWHSDMHCVEQNVSTGDQLQNAGLNRWVPFHGCA
                     NFGLEPSYAFRSAMTAGNILISGLAPGEKEAVKRSVARYHELRPYMLGDFYPLFPHTD
                     NDDAWFGYQFHRPETGDGFAMVFRRPKSEEGTKTVCLRAMERDLWYHVRIEGSSNCQH
                     FIGWQLEDLSVNITECPGTSLIRYAPAESESEG"
     gene            complement(2730..3875)
                     /locus_tag="P5179_11565"
     CDS             complement(2730..3875)
                     /locus_tag="P5179_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95898.1"
                     /translation="MLQWKFFSERVQYHDPTTGIKVIQLTNYPSPAAHFPYDWPSITP
                     DNHYILLYAQRWTQRNAPWDIFRVEADGLNLFQLTEYGDQAADAGYYGRPHATLTHDG
                     RHVYVLWADRLHRIDVETGADEELLSLEEYATPGSFFGRMCLDSTGKTMFLSRWGTST
                     TLRIRLDSGAIDQIELGGIVLGCFQNDDRLEVLRNTGQVEPVTQADGSRVFQNVNATP
                     SSVWSIRPDGTDAQFLSRIDQFGHHTILGQTGLLQGTGQPPHRCIWIVEAGKEPHKLV
                     EGPYFWHSGASFNGEWIVADTNWPDQGIQLIHVPTRHFRTLCHARATQDHVEYGHPHP
                     TISPDGKLVVFRSDRTGVSQVYVAHVTEEFRESVIAGELDRPRDKWI"
     gene            complement(3915..>4353)
                     /locus_tag="P5179_11570"
     CDS             complement(3915..>4353)
                     /locus_tag="P5179_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95899.1"
                     /translation="RMDMFGHHTILGQTDRLQGTGQPPHRCIWIVESGKEPFKLAEGP
                     YFWHSAASFDGEWIVADTSWPDEGLKLVHVPTGHWRTLCHPHAEQDHAGVHPHPGISQ
                     DGRKVVFASDRTGVPQLYIAHITEEFRQSVIAGELDRPRDKWI"
BASE COUNT          798 a         1328 c         1310 g          917 t
ORIGIN      
        1 ccaggtgtgg ttcgcagttc ttaccaccca gccgtggttg tagaatttcc agatggtggc
       61 gccggcggca acccgctcgg tgcggatctg cgaaattgcc aagtcaacat tcgatcggat
      121 gaactgtttc accggctcga gtgcgggcgc actgcggata tgcaacggct catccagcat
      181 gcacagtgcg tggcgccgca gtatctggtc gccaccgttg ggcgggtttt tccgcaacgt
      241 cttctgaacc caaccgagat ttgcctccat ccatcggttc aggaggggaa ggtacccggg
      301 gtcgagatcg gggagcgcgg tctcaatcac gccttcaccg acatggcgcc agctttcgaa
      361 aacccactgg tcatcctttt tgcgaagaga gaagctgaac accccgagtc cccaccccgt
      421 gagggcgtac acaggttcga ccaccgccgc cgaacccgta acttgaacgg ctgcccgggc
      481 gaagttgaaa ctcgttcgcg gagccatgcc gcgcgtgtct tgttccggtg cctgtttcca
      541 ttgttccaat gcaaggcgaa tctgctgttc ttcgctgttc accggtgcct ccgttcgttg
      601 tgcgccgcgg ccggtgatgg ccgcacatcc gatacacacg atcgttatta ccacgcccac
      661 caacgcgtag ctcgacactc ctaccgacct tggcatcatc ttcttcccat tgacgcggtc
      721 cgttcaaccc tctgattcac tttccgcggg agcgtacctg atcaggctgg ttccggggca
      781 ttcagtaatg ttcacagaaa ggtcctccag ttgccaacct atgaaatgct ggcagttcga
      841 actgccctca atccgcacgt gataccagag atcgcgttcc atggccctca gacacacagt
      901 ctttgtcccc tcttcgctct tcgggcgtcg gaacaccatc gcgaatccgt ccccggtttc
      961 cggtcggtgg aactggtacc cgaaccacgc gtcgtcgttg tcggtgtgtg ggaagagggg
     1021 gtagaaatcc ccaagcatgt aaggccgcag ctcatgatac cgcgctacgc ttcgtttcac
     1081 cgcctctttc tcgcccggcg ccaaccccga gatgaggatg tttcccgccg tcattgcgct
     1141 tctgaacgca tacgaaggct cgagcccgaa attggcgcac ccgtggaacg ggacccatct
     1201 gttaaggccg gcgttctgca actggtcgcc cgtggaaacg ttctgttcca cgcaatgcat
     1261 atcgctgtgc cagagcggaa ggccgtaccg ggcggcctcg atgtcgaggc gtcgcccgcc
     1321 gctggaacag ttatcgatga gaaggcccgg gtggcggcgt tcgagttcct cccacatcgc
     1381 atagagcccc tcgatgtgcc ggatttcggt tatcccgact cgatcaggcg tgtcgtttcg
     1441 ctgccagaag gtgagcggct cgatgttgaa atcccacctg agccatgcca gttgcgcttc
     1501 ggttacgaat ctgtcaatga ggtcagtcaa ccatgccctg gcaacggggt cgtgcatgca
     1561 gaaaagctgg ctccattcgc cctcggcctt cattacccac tcgggatgct cgcgctccac
     1621 cattgtcccc gggtgcacgc gctcgggttc gaaccagagc aggtagccca taccggctgc
     1681 tcggacggca tccccgatcg gtttcagacc attcgggtat ttggccctgt cgatctccca
     1741 gttcccggtg ccctcaggga aaccgatcgg ataccattgc tgcgggtcca tatccgacca
     1801 gaacacctca atgccgcgct ctgccatggg gcccacggcg tcgaggtgag gtttctcgta
     1861 cgtcccgtcg gcggcgaccg tggcgacgga acagcagatc ggaggaaaga ccggcttgcc
     1921 gtcacgtctg gggtaatagt gtttgatcgc caggcgccgg aacaggttgt tgcctctcag
     1981 atgatcgtcg ccgtcccaga aaacaaggac gattctggga caccgaacac tctcgccagg
     2041 gtggaggagg aaatgcgtcg tctgctggcc ggcgcggacg cgcatatccc cggtgctggt
     2101 taggtggaac cccgcctccc actggccgga ccaccctatg gcgagcacga tgccgccggt
     2161 tttccaatcc atgttgaaat acggcatgtg gccatccgat gaccggccgc ctttcggggc
     2221 gaacacgcgc tcggctgctc caggtacgag aggctcgtcc acgggcgcga agctttgagc
     2281 ggagttggaa tcgccaaggg aacggtgcaa cacacatccc ttggtgccga gtttcagggc
     2341 catgtccaga gcccggatgt cggaaatgat cggcgtgtcc tgcgtgccgg tgttggtgaa
     2401 cgtcaggagc cactccacgg ccggaaggtc gtggtactca gtagcagtgc agcgcacttc
     2461 aagcccggtg cggtcgtccc gccagacgga tgtgagttcc gtcctctcgg cgtccaacgt
     2521 gcgcgattcc gattcgcggc gcaaactacc aaggaactca gaagaaacgc gtccaccgta
     2581 gacgaaagaa aacggaggct caccggagcc cttgattgct gtgcccgtga tccaatcggc
     2641 cttcgcccgc atctctgccg ggtgcacatc gttccttaac cgggtatcag accccatctc
     2701 ggtgatcctt tcgccatctc gcagacggac tagatccatt tgtctcttgg ccgatcgagt
     2761 tctccggcga ttacgctttc gcggaactcc tcggtgacat gcgccacgta cacctgcgac
     2821 acgccggtac ggtccgatcg gaacaccacc agcttcccgt ccgggctgat ggtgggatgg
     2881 ggatgcccgt actcgacgtg atcctgcgtt gcccgggcat ggcagagcgt ccggaagtgc
     2941 cttgtaggaa cgtggatgag ctggatgccc tgatccggcc agttggtatc cgcgacaatc
     3001 cactcgccgt tgaacgaggc ccccgagtgc cagaaatagg gcccttcgac gagtttgtgc
     3061 ggttccttcc cggcttccac gatccagatg cagcggtgcg gcggctgccc tgtgccctgc
     3121 aagaggccgg tttgcccaag gattgtgtga tggccaaact ggtcgatgcg cgaaaggaat
     3181 tgcgcgtctg tgccgtctgg cctgatgctc cacacagagg agggtgtcgc gttgacgttc
     3241 tggaacaccc gcgagccgtc tgcctgtgta accggttcca cctgcccggt attccggaga
     3301 acttccagcc gatcgtcatt ctgaaagcag ccgaggacaa tgccgccaag ttcgatttgg
     3361 tcgatggcgc ctgaatccag ccgaatccgg agagtcgtcg acgtgcccca acgggagaga
     3421 aacattgtct tgccagttga atcgaggcac atccggccaa aaaacgatcc cggtgtcgcg
     3481 tactcttcga gcgagagaag ctcctcgtct gcgcccgttt cgacgtctat cctgtgcaac
     3541 ctgtccgccc agaggacata gacgtgcctg ccgtcgtggg ttagtgtcgc gtgcgggcgc
     3601 ccgtaataac cagcgtcagc ggcctggtcc ccgtattcgg tcaactggaa gagattcagc
     3661 ccatcggctt ccacgcggaa gatatcccac ggggcgtttc gctgcgtcca acgctgggcg
     3721 tacagcagga tgtagtggtt atccggcgtg atgctgggcc agtcgtaggg gaaatgagca
     3781 gccgggctgg ggtagttggt gagctggatg actttgatgc cggtcgttgg gtcgtggtac
     3841 tggacccgct cactgaagaa tttccattgc agcatacgtg tgatcccttg ccttgggcct
     3901 tggatgacgg acgttcaaat ccatttgtct ctgggacgat caagctcccc ggcgatgacg
     3961 ctttgccgga actcttccgt gatgtgcgcg atgtagagct gcggcacacc ggttctgtcg
     4021 gaggcgaaga caacttttcg gccgtcctga ctgatgcccg ggtggggatg gacgccggcg
     4081 tggtcctgct cagcatgggg atggcagagc gtccgccagt ggccggtcgg cacgtgcacg
     4141 agtttcagac cctcgtcggg ccaggaggta tcggcgacga tccactcgcc gtcgaatgac
     4201 gccgcggagt gccagaaata gggtccttcg gcgagcttga aaggttcctt tcccgattcg
     4261 actatccaga tgcagcgatg gggtggttgc cctgtgccct ggagtctgtc ggtctgcccc
     4321 aggatcgtgt ggtggccgaa catgtccatg cgc
//
LOCUS       DAOTNG010000269         4344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16435_length_4344_cov_5.926323, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000269 DAOTNG010000000
VERSION     DAOTNG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_4344_cov_5.926323"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="P5179_11575"
     CDS             complement(<1..500)
                     /locus_tag="P5179_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95900.1"
                     /translation="MRATVFAVLFMVANGSTAMDVLVRPPFDAASPALVYASSRVQAA
                     AEKAGLAGTAPRVMVEMVAADALQPESFTIAPSSDRRTVLITAADARGAAWGLLWLAD
                     RITVEGRDAFTRRWERTPAFRRRFLLDGASYRTPPGSPPEATLELDLRFTCNTHAHSF
                     NTSGAAG"
     gene            934..3237
                     /locus_tag="P5179_11580"
     CDS             934..3237
                     /locus_tag="P5179_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS95901.1"
                     /translation="MITANPEPQRSLKTETLECDLAVVGGGLAGVCAALTAARAGLKV
                     VLMQDRPVLGGNASSEVRLWGLGATCHMGSNNRWARESGVIDEILVENHFRNPEGNAV
                     IFDTVLLEWVLREPNITLLLNTAAFEVAKSDDSTIARVKGFCAQNSVLYDVGAKLFCD
                     ASGDGILGFLSGAAFRMGAEAKEEFNEPLAPSIEYGELLGHSIYFMSKDVGVPVKFVA
                     PSYALKDIKKIPRFKNITAEAGSHKCHLWWFEYGGRLDTVHDTEKIKWELWKVAYGAW
                     DYIKNSGKFPEAANLTLEWMGCIPGKRESRRFEGDYMLTQGDIIHQHHHEDAVSYGGW
                     SIDLHPADGVYSPLPGCNQWHARGVYEIPYRTMYSRNIANLFLAGRLVSSSHVAFGST
                     RVMLTCAESAQAVGMAAALCVRDKLLPKDLAEPKRMKELQTRLLRAGQYIPGVPHKDV
                     DDLAQSATITATSSLKLSRLDPDGTTKQLTKGQGLLLPLLPGPAPKVTLFADAARPTK
                     VRVELRTSDRPENHTPDVVLGTQEISLPAGENQPLTLAFDATITEPRYALYCVRANEA
                     VSLRCSGERVTGVMAVVHRFTHQHDSALDVETYEVWAPERDPLRRTFACQIDPPLAAF
                     SPQCVANGFARPTCRPNAWVADFSDDDPVLAIAWGIPQTISHIQITFDTDWEHPLESV
                     LWPHHSGLIPSCVKSFRIFGDMGALLYEEKNNHQTRVMVALPKPVRASRITIECVANW
                     AEEMGIKVPPAAIYDVQVYEHDPGRIV"
     gene            3269..4144
                     /locus_tag="P5179_11585"
     CDS             3269..4144
                     /locus_tag="P5179_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95902.1"
                     /translation="MQGIRKLGKVQDNLVEVSPFVFKGELLLFESVRPDTPDNTHGGR
                     HYLRIRRLPGGTRDVTGPEEFAACPVLTEFGEEYTFGVPFVWNDEIFIYASLGNKPAV
                     DDIHVFHSRTMDNWQHSIAVRGADEQVFNSSVCRAGDRFIMAYESNFSAEWVPFTNRF
                     AESKDLVHWTPISPDRAVHGSDRYCACPTLRYIDGTFVIICLEMPHRGAWWFEEFAAT
                     SKDLWNWTMSPANPILAPPDDRSENINNSDIDFCGFGDKTIIYYSWGSQRGDEHLAHA
                     VYDGPEEAFLRAMIG"
BASE COUNT          851 a         1452 c         1294 g          747 t
ORIGIN      
        1 cccgccgcgc cggacgtgtt gaacgagtgc gcatgggtgt tgcaggtgaa gcggagatcc
       61 agctccagcg tcgcctcggg cgggctcccc ggtggcgtgc ggtaggaggc gccgtccagc
      121 agaaaccgtc gccggaatgc gggtgtacgt tcccaccgcc gtgtgaaggc atctctcccc
      181 tccacggtga ttcggtctgc cagccacaga agcccccacg ccgcgccccg cgcgtctgcc
      241 gcggtgatca gcacggtgcg tcggtcgctt gaaggcgcga tcgtgaagga ttcgggctgc
      301 agtgcgtccg cagccaccat ttcaaccatt acccgaggtg cagtgccagc caatccggcc
      361 ttctccgccg cggcctgaac gcgcgacgac gcgtacacaa gcgccggcga cgccgcgtcg
      421 aacggtggac gaaccaggac atccatggca gtactcccgt tggcaaccat gaacaaaaca
      481 gcgaaaacag tggcgcgcat gacgttctcc ggagggtgac ggggcaacgc gttccccgcc
      541 gggaaccacg cgcgaggcaa cacgtcagcc gtaagcatac cggctgcccg gtttcggtca
      601 aacccttgcg ggccatccga acacctgcct ccagtatggc tgtggcaaaa cggcgtgctc
      661 gtgtcaagag caaactgcaa aaaagttcat tttcggtacg ggccggccag tcccgcgcgc
      721 tcagcaccgc acggaccggt cgcccctgcc gccacacgcc ccgccacacc ggtaccatcc
      781 ctccgtcaac cttccccgca ccacacagaa acccgttggc ctcggcgcgc accatcccgt
      841 tccgtctcgc gtccgttcgg cgtacacttg tcagtatgag taagatctgg cggggggcag
      901 ccgttgtatc gcgagtgtga gggagatcag gcgatgatca ccgcaaatcc ggaaccgcag
      961 cgcagcctga aaaccgagac gcttgagtgc gacctcgccg tcgtcggtgg tggcctcgcc
     1021 ggcgtgtgcg ccgcactgac ggccgctcgt gcgggcctca aggtcgtcct gatgcaggac
     1081 cggccagtgc tcggcggaaa cgcgtcaagc gaagtccgcc tctgggggct cggcgccacc
     1141 tgccacatgg gcagcaacaa ccgctgggcg cgtgaaagtg gggtaattga cgaaatcctc
     1201 gtcgaaaacc acttccgcaa ccccgaaggc aacgccgtta tcttcgacac cgtgcttctg
     1261 gaatgggtgc ttcgggagcc gaacatcacc cttctgctca acaccgccgc gttcgaggtc
     1321 gccaaatccg acgacagcac catcgcccgc gtaaaaggct tctgcgctca gaactccgtc
     1381 ctgtacgacg tcggcgcgaa gctcttctgc gacgcatcag gcgacggcat cctcggcttc
     1441 ctttccggcg cggccttccg catgggcgcc gaggcgaagg aggagttcaa cgagccgctc
     1501 gccccctcaa tcgaatacgg cgaactcctc ggccactcga tctatttcat gagcaaggac
     1561 gtcggcgtgc cggtcaagtt cgttgccccg agttacgccc tgaaagacat caagaaaatc
     1621 ccccggttca aaaacatcac cgccgaggcg ggctcccaca aatgccatct ctggtggttt
     1681 gaatacggcg gacggctcga tacggtgcac gacaccgaaa agatcaagtg ggaactctgg
     1741 aaggtcgcct acggtgcgtg ggattacatc aagaattccg gcaagttccc cgaagcggcc
     1801 aacctgacgc tggaatggat gggctgtatt cccggcaagc gcgaaagccg gcgctttgaa
     1861 ggggattaca tgctcactca gggcgacatc atccaccagc accaccatga ggatgcagtt
     1921 tcctacggcg gatggtccat cgatcttcac cctgcggacg gcgtctactc tcccctgccc
     1981 ggttgcaacc agtggcatgc gcggggcgtg tacgaaatcc cttaccggac gatgtacagc
     2041 cggaacatcg ccaacctgtt cctcgcggga aggctggtca gctcctccca cgtcgcgttt
     2101 gggtccaccc gcgtgatgct gacatgcgcc gaatccgcgc aagcggtcgg gatggccgcg
     2161 gcgctgtgcg tgcgggacaa actgctcccc aaagacctcg ccgaaccgaa gcgcatgaag
     2221 gaactgcaga cacgcctgct gcgggcgggg cagtatattc ccggcgtgcc gcacaaagac
     2281 gtggacgacc tcgcccaatc ggccaccatc accgcaacca gcagcctgaa gctctcccgg
     2341 ctcgaccctg acggcaccac gaaacaattg accaaaggac aggggctgct gctgccgctc
     2401 ctgccggggc cggcgcccaa ggtaaccctg ttcgccgacg ccgccagacc gaccaaagtt
     2461 cgcgtcgaac tccgtacaag cgaccgtccg gagaaccaca cgccggacgt cgttctgggc
     2521 acgcaggaga tatctcttcc cgccggcgag aaccagccgc tcacgctggc tttcgacgcc
     2581 accatcaccg agccgcgcta tgcgctgtac tgcgtccgcg cgaatgaggc ggtcagcctg
     2641 cgctgctcgg gcgagcgggt caccggtgtg atggcagtgg ttcaccgctt cacgcatcag
     2701 cacgactccg cgctcgatgt cgagacgtac gaggtgtggg cgccggaacg cgacccgctc
     2761 cgccggacgt ttgcctgtca gatagatccg ccgctcgcgg ccttctcgcc gcagtgcgtg
     2821 gccaacggct tcgcccgccc cacctgtcgg cccaacgcat gggttgccga cttctcggat
     2881 gacgacccgg tcctcgccat cgcatggggc ataccgcaga cgatttccca catccagatc
     2941 acgttcgaca ccgactggga gcacccgctg gaatccgtcc tgtggcccca tcacagcgga
     3001 ttgattccct cctgcgtgaa gagcttccgg attttcggcg atatgggcgc cttgctctac
     3061 gaggagaaaa acaaccacca gacccgcgtg atggttgcgc tcccgaaacc agtgcgtgca
     3121 tcgcgcatca ccatcgagtg cgtggcaaac tgggccgagg agatggggat caaggtgccg
     3181 cccgccgcca tttacgacgt gcaggtgtac gaacacgacc cgggcaggat cgtctgacca
     3241 cccgtgccac ggcagaaaag aggctctcat gcagggaata cgcaaactcg ggaaagtgca
     3301 ggataacctc gtggaagtct ccccgttcgt gttcaaaggg gagctcctgc tgttcgaatc
     3361 ggtccgcccc gatacgcccg ataacacgca cggcgggcgg cactatctgc gtatccgccg
     3421 ccttccgggc ggcacgcgcg atgtaacggg accggaggaa ttcgccgcgt gcccggtgct
     3481 gaccgaattc ggcgaggagt acaccttcgg cgtgccgttc gtctggaacg atgagatttt
     3541 catctacgcc tcacttggca acaaacccgc cgtggacgac atccacgtct tccattcccg
     3601 caccatggac aactggcagc attccatcgc agtgcgcggc gcggacgagc aggtcttcaa
     3661 cagcagcgtc tgccgggcgg gcgaccggtt catcatggcg tacgaatcca acttcagcgc
     3721 ggaatgggtg cccttcacga acaggttcgc cgagtcgaag gatctcgtgc actggacacc
     3781 gatttcgccc gaccgagcgg ttcacggttc tgaccgatac tgcgcctgcc ccaccctgcg
     3841 ctacatcgac ggcacgttcg tcattatctg cctcgaaatg ccgcaccggg gcgcatggtg
     3901 gttcgaggag ttcgcggcaa cgtcaaagga tctctggaac tggaccatga gcccggccaa
     3961 cccgatcctt gcgccaccgg acgaccgttc ggaaaacatc aacaactccg atatcgactt
     4021 ctgcggtttt ggcgacaaga caatcatcta ctactcctgg ggcagccagc gcggcgacga
     4081 gcacctcgcc catgcagtct acgacggccc cgaggaggcg ttcctccgcg ccatgatcgg
     4141 ctgaacaatg cctgtcagac ctgacgaaac gggtccgatg ggccgtacgg gcgcggaatc
     4201 gcttgggggc acgcaagggg agattgctcg cttcgcgcag aatagtgttt ctatctcggg
     4261 cccacggagt ttggcggccc gtactgacaa cggaggaccc attcatgcga cgtttccttt
     4321 cccttttctg tgcgctggtg ttcg
//
LOCUS       DAOTNG010000270         4319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16553_length_4319_cov_4.823640, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000270 DAOTNG010000000
VERSION     DAOTNG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16553_length_4319_cov_4.823640"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1212)
                     /locus_tag="P5179_11590"
     CDS             complement(10..1212)
                     /locus_tag="P5179_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRS95903.1"
                     /translation="MKPHAVSWDAKTFGARGDGARDDTASFQAGLDAVASGGGGTLSV
                     PPGRYRICGPLEVRANVTLQGAGGAVNGWGEPTVFEVFDREHPFLSLSNGGGVHNIDI
                     VYPEQRLRAGEVVPYPYTIVARGENCHVIGVSLRNPYNGICLDYAARHLVRDVHGSPL
                     NIGLFVNQCYDVGRVENVHFWPFMGPEENKEFYLEYLPHHATAFVFGRTDWEYVFNTF
                     CWGYKVGYHFAETEQGSTNGNFVGIGADATEMCLLVDQAQPCTGGILVTNGEFVPLMS
                     PDCSALVVGETFRGYISLVNCGVWGPHDSSARIAGDGRVNIASCNFIDWDKNLTDAPV
                     IDCRGGVLTLNGNHFTRSQNKNRSTPQVALRRGTRGAIVTGNFSDNPWRVINEIGDAA
                     QIGFNLGT"
     gene            complement(1231..2124)
                     /locus_tag="P5179_11595"
     CDS             complement(1231..2124)
                     /locus_tag="P5179_11595"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRS95904.1"
                     /translation="MTPAKDLWAFWEATKAELADTPADATMTPAPEMSSESALIYHVS
                     MAGFGGRRIGGWFGTPPVPPGVRIAGLLWLPGYGGVPEAATALDFASKGFATLVLEPR
                     GQGKGNAFPDGSDGKLVHGITDPHGYAYRAVVMDCVRGLQILQNQPEVHPDRVAVAGG
                     SQGGGLTLMTASVCDESVRVVAAHVPFLCHFDFVIEHAIETAPYVNLTRYLAEFPERR
                     EIARRTLSYFDPVNLVSRLRAPVLLTLGGRDTTCPRETIEAVFEAVPGKKALLVEPDA
                     RHEWLDDTRTHMVSWLNHYLG"
     gene            complement(2121..4193)
                     /locus_tag="P5179_11600"
     CDS             complement(2121..4193)
                     /locus_tag="P5179_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS95905.1"
                     /translation="MVVNRDYTRWLGNLKWMPRDSWIRNMHDPAAECAGNPVTLAGDS
                     GTMWISSPIPIPLDHANLQVSVAGTVRGGRIGIEWCHAKRGKFYPPVWAAADGDGRFL
                     LSQAGARRTTASFRFVVELGNERLAALEITECRGESQPELQVLVNQVGYNVGLPKTFA
                     VQCNAPLEVGGSFRVIAPIAGPGKEFVTVFEGALEPAGRIGEWERTYWRGDFSRLDIP
                     NNYYIEARVGDLEAESPVFALGNNFVAERTLDTAQRFYWYQRCGMAIPGWHEACHLDD
                     RITTADGYTRDVAGGWHDAGDYNKYNGFTPHSVWALLYTHEQRADLCRTLRNGPHCDI
                     LDEALWGAEFLRKMQEPVSGKLWDRVFSGYGHWNTPESETDNRFGDEKDRVAAGEARN
                     LWLVASFAHIGRVLRNAEFTDRALRHWKLCRDLGNEGLHHNAAQLIAAHELLRTTGDT
                     GFVEVEEQCVRHILACQGADENYDGWFANAPGGKPAYSIVDNGLPAASLALWVRANPT
                     RNLAVDVLGSLVRYTGFLTRIADNPFGISKFYTGDAEAFFYQFPEESSWHVGQNSQYL
                     SEAWAAFLIFQVTHRKEALSHGLKQLDWVLGANPYGICMLHGEGRVNTATAHHRYNTI
                     VGQPQGAVPGSVFNGITRFRPECDVPLWDLLEKGTPAYECNEPWIPHNAYYLLAVTEL
                     AAVCEGIR"
BASE COUNT          883 a         1363 c         1190 g          883 t
ORIGIN      
        1 tttccggctc tacgtcccga gattgaagcc gatctgcgcg gcgtcgccta tctcgttgat
       61 gacacgccag gggttgtcgc tgaaattacc ggtaacgatt gcgcctcgtg tgcccctccg
      121 caacgccact tgaggagttg accggttctt gttctgagac cgggtaaaat gattgccatt
      181 cagcgtcaaa acgccgcccc ggcaatcgat aacgggggcg tccgtgaggt tcttgtccca
      241 gtcgatgaaa ttgcacgacg cgatgttgac ccttccgtct ccggcgatcc gcgcgctgct
      301 gtcatgcggt ccccacacgc cacaattgac gagactgatg taaccacgaa atgtctcgcc
      361 gaccaccagc gccgagcaat cgggtgacat cagcggcacg aattcgccat tcgtgacgag
      421 gatgcccccg gtacagggtt gcgcctgatc caccagcagg cacatttcag ttgcgtcagc
      481 accgataccc acaaagttgc cgttcgtcga accctgttcc gtctccgcga aatgatagcc
      541 aaccttgtat ccccagcaga acgtgttgaa cacgtactcc cagtctgttc gaccaaagac
      601 gaatgccgtc gcgtgatgcg gaagatattc gagatagaac tccttgttct cctcaggacc
      661 catgaaaggc cagaaatgta cgttttccac ccttccgaca tcgtaacact gattgacgaa
      721 caggccgatg ttcagcgggc ttccatggac gtccctcaca aggtgccgcg cagcgtagtc
      781 gagacatatg ccattgtacg ggttacgaag tgacactccg ataacatggc agttttcgcc
      841 tcgtgcaacg atcgtgtagg ggtatggcac aacctcaccc gcgcgaagtc gttgctccgg
      901 atacacgatg tcgatgttgt gcacgccgcc gccgttcgag aggctcagaa acgggtgttc
      961 acgatcgaac acttcgaaaa cggttggttc accccacccg tttaccgcgc cgcccgcgcc
     1021 ctgcagggtc acgttggccc gcacctcgag cgggccgcag attcggtacc ttccgggggg
     1081 aacggacagc gtcccgcctc ctcctgaagc aacggcgtcc agaccggcct gaaacgatgc
     1141 cgtatcatcc cgcgcaccgt ctcctctggc tccgaacgtc ttggcgtccc acgagaccgc
     1201 gtgtggcttc atgtccgacc tcatactgct tcagccaagg taatgattca accacgagac
     1261 catgtgcgtt ctcgtgtcat ccagccattc gtgcctcgca tcgggttcga caagaagcgc
     1321 tttctttccc ggcacggctt cgaacaccgc ctcgatcgtc tcccgcggac aggtggtatc
     1381 gcggccgcca agcgtcagaa gaaccggtgc acggagtcgc gacacgagat tgaccgggtc
     1441 gaagtaactc aacgtcctcc tcgcgatttc gcgccgttcc gggaactccg cgagatagcg
     1501 tgtcaagttt acgtacggag cggtttctat cgcgtgctca atgacgaaat caaagtggca
     1561 caggaagggg acatgcgcgg ccaccacgcg gacgctctcg tcgcatacag aggcggtcat
     1621 cagagtcagg ccaccgccct gactacctcc cgcgactgca acacgatcgg ggtggacttc
     1681 gggctggttt tgaaggatct gcagaccgcg cacgcaatcc atcactacgg cccggtacgc
     1741 gtagccgtgc ggatcagtta tgccatgcac aagcttcccg tccgacccat ccgggaacgc
     1801 gttgcctttg ccctgaccgc gaggttcaag aacaagcgtg gcaaaccctt tggacgcgaa
     1861 gtcgagcgcg gttgccgctt cggggacacc tccgtacccg ggcagccaca gcagacctgc
     1921 gatcctgaca ccgggaggaa cagggggagt accgaaccag ccgccgatgc gacgcccgcc
     1981 gaaacctgcc atgctgacat gatatattaa cgccgactcg gacgacatct ccggtgcggg
     2041 agtcatcgtc gcgtcagcag gggtgtcggc cagttcggct tttgtcgctt cccagaatgc
     2101 ccagagatcc tttgcaggag tcatctaatg ccctcgcaga ccgcggccag ctctgtcacg
     2161 gccagaaggt agtacgcgtt gtggggtatc cacggctcgt tgcactcgta cgccggtgtt
     2221 cccttttcga gcaaatccca gaggggcacg tcacattctg gacgaaatcg cgtgatcccg
     2281 ttgaacacgg aacctggaac cgcgccctgc ggttgtccca caatcgtgtt gtaacgatgg
     2341 tgtgcggtag ccgtgttcac ccggccctca ccgtggagca tacagatgcc ataggggttt
     2401 gcgccgagaa cccagtcgag ttgcttgaga ccgtgagaaa gcgcttcttt ccggtgagta
     2461 acctgaaaga tcagaaaggc agcccacgcc tcggagagat actggctgtt ctgaccgaca
     2521 tgccagctcg attcctccgg gaactggtag aagaacgctt ccgcgtcccc ggtgtagaat
     2581 ttcgaaatgc caaatggatt gtccgcgata cgggtaagga aacccgtgta tcggactaag
     2641 ctcccgagaa cgtcaacggc gaggttcctg gttggattcg cccggaccca gagcgccagc
     2701 gaggcggcag gtagcccgtt gtcaacgatt gaatatgccg gtttgccgcc gggcgcgttt
     2761 gcgaaccagc cgtcgtagtt ctcgtcggct ccctggcacg caaggatatg ccgcacacac
     2821 tgctcctcga cctccacaaa ccccgtatca cctgtagtgc gcaggagttc gtgcgcagcg
     2881 atcaactgcg ccgcattgtg gtgaagcccc tcgttcccca gatcccggca gagcttccag
     2941 tgccgcaacg cgcggtcagt gaattctgcg ttccgaagaa cccgcccaat gtgggcaaaa
     3001 ctcgccacca gccacagatt tcgtgcctct cccgccgcaa cgcggtcttt ttcgtcaccg
     3061 aaccggttgt ctgtttcgct ttccggggtg ttccagtggc cgtagccgga gaaaacccgg
     3121 tcccaaagct tgccggaaac cggttcctgc atcttccgaa gaaactcggc tccccagagc
     3181 gcctcgtcca ggatgtcgca gtgtggtccg tttcgtaagg ttctgcaaag atcagcgcgc
     3241 tgctcatgcg tgtagagaag cgcccagacg gaatgaggtg tgaacccatt gtatttgttg
     3301 taatccccgg cgtcgtgcca gccgccagcc acatctctcg tgtatccgtc tgccgtagtg
     3361 atccggtcat ccagatggca cgcctcgtgc cagccgggaa tcgccattcc gcacctttgg
     3421 taccagtaga agcgctgcgc cgtatcgagc gttcgctccg caacgaagtt gttccctaac
     3481 gcaaaaaccg gggattccgc ttccagatct cctacccggg cttcgatgta gtaattgttc
     3541 gggatgtcga gacgtgaaaa atcgccccgc cagtaggtgc gttcccattc accaatccgc
     3601 ccggcgggtt ccagagcacc ttcaaacacc gtaacgaatt ccttacccgg tcccgcgatt
     3661 ggagctatga cgcgaaagga ccctcctacc tccaacggtg cgttacactg gacggcaaag
     3721 gttttcggaa gtcccacgtt gtacccgacc tggttgacga gaacctgtaa ctcgggctgc
     3781 gactcaccac gacattcggt tatctcgaga gccgccaatc gctcatttcc cagctcaacg
     3841 acgaaccgaa aactcgcggt ggttcttctc gcgcctgcct gcgaaaggag gaaacgcccg
     3901 tccccgtcag ccgcggccca gaccggtggg tagaacttgc ctcgcttcgc gtggcaccat
     3961 tcgatgccaa tcctgcctcc ccgcacggtt cccgcgacgg acacctggag gttggcgtgg
     4021 tcgagaggaa tcggaatggg ggaggatatc cacatggttc cggaatctcc cgcaagcgta
     4081 accggattcc ctgcacattc cgcggcaggg tcatgcatgt tcctgatcca tgaatcgcga
     4141 ggcatccatt tcaggttgcc caaccaacgc gtgtaatcgc ggttcactac catcctgttc
     4201 gctctcccgg gtgtttcgaa aatctcaccg gtccatttca agaatcacct tcacgcaccc
     4261 ttcacgcctg tctgcgaaaa ggtcatacgc ttcctgaacc ctggtgaggg ggaacgcgt
//
LOCUS       DAOTNG010000271         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16716_length_4285_cov_4.396927, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000271 DAOTNG010000000
VERSION     DAOTNG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16716_length_4285_cov_4.396927"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1495)
                     /locus_tag="P5179_11605"
     CDS             complement(353..1495)
                     /locus_tag="P5179_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95906.1"
                     /translation="MLRTPFASERISMTDPTTGIRVIQLSSYPLPAAHFPYYWPSITP
                     DNRWILFYSQRFLQRNAPWDIFRVDADGLNLFQLTERNDVQETHGYYGRQYALLSHDG
                     KTLYVLWGNTLYAVDVETGNEELIACMDHLVGEGDALSYCVLSHEGRYIFFRKVSSFV
                     TSVRLDLRTGKAEDVELGGIAMGAMQTEPRVMVQAGEIIWGSEPTPDGGRRITNLGSK
                     PVLYSVAEDGSDRRLICPNIFAHCTLLGKTSKVQGCGLPPERCIWIAEEGSEPRKLCS
                     GPYFWHSGASYDGEWIVADTNWPDCGLQLVHVPTGHFRTLCHTGATLEHYEFGHSHPL
                     ISNDGRLVVFRSDRTGCPQVYVAHVTEEFRQSIIAGELDRPKDKWI"
     gene            1687..3693
                     /locus_tag="P5179_11610"
     CDS             1687..3693
                     /locus_tag="P5179_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS95907.1"
                     /translation="MAFPGPGNTNSVSRSEADPAYLLGSIRTVLSDPYFGGIEITHIK
                     SHEDRRAVADLIREMRPNGMEITFACQPVQLINEENLIAPSDISSVDDNEREKAVARI
                     LSLLEEAAEVGATKLTLLSGRDPAYGIEDAKQAAEIREMGRVGIVRSLDTICRVAKPL
                     GITIVLEIFDNREEADGPKAFKGQFIGPTPDAVRLAENIRYERNYQNFGLLYDSSHMV
                     LMQETPEALKALKPYLAHVHVANCVTSRTAKDADQRYGDRHPKFGAAGSAVDSTVLAE
                     FVQALVEIDYRGTIGFEVAPIGSESPTDVIATAKAFFDEARNSVNVAYARPSGYAFRS
                     HRFFPEAALARVNEIRVEQPELVEELLSVRPRREKLTPDGRLTILAADHPARNVTQVG
                     DDPVAMGNRLDYLGRIMRVLVASEIDGLMATSDVIDDVVLADYVLQECGKPSVLEKRL
                     LIASMNRTGLAGAEYEMMDKMSSYRSAKRIAQMNLDGAKLLLRISAPDKYDRYVLQTI
                     DWCAEAIEQCNDLKLPVFLEPLPVERTETGYRTIKQPDPMIRVIGVAQALSHSTARTW
                     LKIPYTAEFDRVAKATTLPLLLLGGEATGRPWLTVEEFVRGLGAGANVRGALVGRNVL
                     FPGDEDPAVVAQAIHSVVHEHADAVSAMNAARERRGSMMDFFAL"
     gene            3804..4025
                     /locus_tag="P5179_11615"
     CDS             3804..4025
                     /locus_tag="P5179_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRS95908.1"
                     /translation="MTKNEGSVDRIVRVILGVVLIVWAYVIGQGQWSTWSWILGIVGL
                     IALITGIIGWCGLYAVLGINTCKVPKKEG"
BASE COUNT          775 a         1346 c         1381 g          783 t
ORIGIN      
        1 gagctatttc gcgccgttgg cgctggtgag cggggtgtgt gccggccctt cgacaagctc
       61 agggaccggc gtgcaactcg gcaggggttc ccagtccact gagtccaacg ggaaggcctg
      121 aagggccgca atatctcagc ccggctcatc gggccggggg tgtgtccagt ctcctccgtc
      181 caccgcgcct gtcggtcgtc tcgatacaac gagcgctgaa atgcgcgctc gtcactcgac
      241 gaccgatttg gttcgcgccc gacgaccggt ttggtcgccg ccggcggttc gacacgctcc
      301 cgccctccgg gagcccgctc agggaccggc ccgcaactcg acaaccgtct cctcaaatcc
      361 acttatcctt cgggcggtcc agctccccgg cgatgatgct ctggcggaac tcctccgtca
      421 catgcgccac gtacacctgc gggcagccgg tgcggtcgga gcggaacacc accagccgcc
      481 cgtcgttgct gatgagcggg tgtgaatgcc cgaattcgta gtgttccagc gtcgcgcccg
      541 tgtggcacag ggtgcggaag tgcccggtcg gcacatgaac gagctgcaga ccgcaatccg
      601 gccagttggt gtcggcaacg atccattccc cgtcgtacga cgcgccggaa tgccagaaat
      661 agggtcccga gcagagtttg cggggctcgc tgccctcttc cgcgatccag atgcaccgct
      721 ccggcggaag cccgcacccc tgcaccttcg acgtcttgcc cagcagcgtg cagtgcgcga
      781 agatgttcgg gcagatgagc cgccggtcgc tgccgtcctc cgccacgctg tacagcaccg
      841 gtttggagcc gaggttggtg atccgccgcc cgccgtcggg cgtgggctcc gagccccaga
      901 tgatctcccc ggcctgcacc atcacgcggg gttccgtctg catggcgccc atggcgatgc
      961 cgccgagttc cacgtcctct gccttccccg tccgcaggtc gagacgcacg gatgttacaa
     1021 atgaggacac cttgcggaag aaaatgtaac gcccttcatg cgaaagcacg cagtaactca
     1081 acgcgtcccc ttcgcccaca aggtgatcca tgcaggcgat caactcctcg ttgccggtct
     1141 cgacgtcaac cgcgtacagc gtgttgcccc acagaacgta cagcgtcttc ccgtcgtgcg
     1201 aaagcagcgc gtactgtcgg ccgtagtacc cgtgcgtttc ctgcacgtca ttccgttcgg
     1261 taagctggaa cagattcagg ccgtcggcgt ccacccggaa gatatcccac ggagcgttgc
     1321 gctgaaggaa ccgctgcgag tagaagagga tccaccggtt gtccggcgtg atgctgggcc
     1381 agtagtacgg aaaatgggcg gcggggaggg gatagctgga aagctgaatg accctgatac
     1441 ccgtggtcgg gtcggtcatg gatatgcgct cgctggcaaa tggcgtgcgg agcatggggg
     1501 cggcttctcc ttcgggttgt ggtgtctgcg ggcaaatccc ttgtgtaacg gggtgttccc
     1561 ccgtacgttt acgttgtacg caagtgcgcg tggacgtctc gtattcggcg ggtctgtttc
     1621 ccgatcgccc tgctcaagga gcgcctcatg gctctcgacc agactaccct gtcaattgtt
     1681 cacttcatgg cgtttcccgg tccggggaac accaacagcg tctcccgctc ggaggccgac
     1741 cccgcgtacc tcctcggctc catccgcacg gtgctttctg atccctactt cggcggcatc
     1801 gagatcaccc acataaaaag ccacgaagac cgccgcgccg ttgccgatct gatcagggag
     1861 atgcgcccga acggcatgga aattacgttt gcctgccagc cggtgcaact catcaacgag
     1921 gagaacctga tcgcgccgtc ggatatctcc tcggtggacg acaacgagcg cgagaaggcg
     1981 gtagcgcgca tcctgtctct tctcgaggaa gccgcggaag tcggcgcgac gaagctgaca
     2041 ctgctcagcg ggcgtgaccc cgcctacggc attgaagacg cgaaacaggc cgccgaaatc
     2101 agggaaatgg ggcgcgtcgg aatcgtccgc agcctggaca ccatctgccg cgtggccaag
     2161 ccgctcggaa tcaccatcgt gctggaaatc ttcgacaacc gcgaggaagc ggacggcccg
     2221 aaggcgttca aagggcagtt catcggcccc acgcccgacg cggtacggct tgccgagaac
     2281 atccgctacg agcgcaacta ccagaacttc ggcctgctgt acgattccag ccacatggtg
     2341 ctcatgcagg agacaccgga ggcgctcaag gcgctcaagc cctacctcgc ccatgttcac
     2401 gtggccaact gcgtaaccag ccgcaccgcg aaggacgccg accagaggta cggcgaccgg
     2461 cacccgaagt tcggcgcggc aggcagcgcg gtagacagca cggtgcttgc cgagttcgtg
     2521 caggcgctgg tggagataga ttatcgcggc accatcgggt tcgaggttgc gccgatcggg
     2581 tcggaatccc ccacggacgt catcgccacg gcgaaagcgt ttttcgacga ggcgcggaat
     2641 tcggtcaacg tggcgtatgc gcggccctca ggctacgcgt tccggtcgca ccggtttttt
     2701 ccggaggccg cccttgcccg ggtgaacgag attcgcgtgg aacagccgga actggtggag
     2761 gagctgctct ccgtccgccc gcgccgtgaa aagctgacgc cagacgggcg cctgaccatc
     2821 ctggcggcgg accaccccgc ccgcaacgtc acgcaggtcg gggacgaccc ggtggccatg
     2881 gggaaccggc tcgattatct cggcaggatc atgcgggtgc tcgtggccag cgaaatagac
     2941 ggcctgatgg cgacgtcgga cgtaatagac gacgtggtgc tggctgacta cgtcctgcag
     3001 gagtgcggga aaccttcggt gctggagaag agactgctta tcgccagcat gaaccgcacg
     3061 ggcctggccg gcgccgagta cgaaatgatg gacaagatgt ccagctaccg ctcggccaaa
     3121 cgcatcgccc agatgaacct cgacggcgcg aagctgctgc tgcgcatttc cgcgccggac
     3181 aagtacgacc ggtacgtgct gcagaccatt gactggtgcg cggaagcaat cgagcagtgc
     3241 aacgacctca agctgccggt gttcctcgaa ccactgcccg tggagcgcac ggaaacaggc
     3301 taccgcacca tcaagcaacc ggatccgatg atccgggtga tcggcgttgc gcaggcattg
     3361 agccactcca ccgcccgcac gtggctgaag attccgtata cggccgagtt cgaccgcgtt
     3421 gcgaaagcca ccaccctgcc gctgctgctt cttggcggcg aggcgacggg acggccgtgg
     3481 ctgacggtgg aagagttcgt gcgcgggctc ggcgccggcg cgaatgtccg cggcgcgctg
     3541 gtgggccgga acgtgctgtt ccccggtgac gaagacccgg cggtggtggc gcaggcgatc
     3601 cacagcgtgg tgcacgaaca cgccgatgcg gtgtcggcga tgaatgccgc gcgggagcgg
     3661 cgcggttcga tgatggactt tttcgctctg taaacgctat attatcgtac gagaatgctg
     3721 gtaaacgtcg ttccggcaaa cagccggtgc cccggtgaaa cgccggggcc aggtaaccac
     3781 ctcaggaccg aaaggaggga atgatgacga aaaacgaggg ttccgttgac cggatcgttc
     3841 gcgtcattct cggtgtggtt ctgattgtat gggcgtacgt tatcgggcag ggtcagtggt
     3901 ccacatggtc ctggatcctt gggatcgtgg gcctgatcgc gctcatcacc ggcattatcg
     3961 gctggtgcgg tctgtatgcc gtactcggca tcaatacgtg taaggtgccg aagaaagagg
     4021 gataacgccg gggacgcgaa cgtccggcga cgaatccgga agggcggggc gtgtattgga
     4081 cgcgccccgc cctttttctg tgggcacgga gggtattgcg ttgccggcgt cctacttatg
     4141 ccgcgtgccg gtgccgcctt tctgatggat tttcggcttc ggttgcgcgt gcggcgcgtc
     4201 tttgcttggc agaaccgggc cgccacgctt atcctgcccc ggtatgcgag gtccttgttt
     4261 ctctctcatg ggaatgccct ttcct
//
LOCUS       DAOTNG010000272         4282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16728_length_4282_cov_5.788739, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000272 DAOTNG010000000
VERSION     DAOTNG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16728_length_4282_cov_5.788739"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..882
                     /locus_tag="P5179_11620"
     CDS             379..882
                     /locus_tag="P5179_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRS95909.1"
                     /translation="MATGPLGVVRRQASFVGNYRHVVDAQGRIQVPRAIRKAMLPESQ
                     GTLYVTRGLDGCLFAYPLDTWQNLAEQWELALNDARSFEKRNEIRVMTSHACEARLDG
                     QGRISIPAYLLKAVDIRSEALFVGVTYRIEIWNPDRYEAAIAAAENSFEQNMRNLVSF
                     RQNRQSG"
     gene            888..1817
                     /gene="rsmH"
                     /locus_tag="P5179_11625"
     CDS             888..1817
                     /gene="rsmH"
                     /locus_tag="P5179_11625"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRS95910.1"
                     /translation="MPEHYRWHVPVLADQAVEALVWRKDGTYVDATVGDGGHAEKICE
                     RLAGSGRLLAIDRDQEALERAADRLNQYKDRVVFARASFARLDELAENANLGPISGML
                     FDLGVSSRQLDQPERGFSYRSEGPIDMRMGPDAETAAATIVNEWPEPELARLFRVFGE
                     EPHAASIARSICQRRNRRPLATTSDLVEAIGRRGTHPEKTLSRVFQALRIAVNDELAS
                     LNRVLGLVPDLLECGGRLVVISYHSLEDRSVKAWMRMETRGCICPPTVPECRCGHVPR
                     MRAITRRAVMPTAEEIAGNSRSRSARMRVAERL"
     gene            1814..2179
                     /locus_tag="P5179_11630"
     CDS             1814..2179
                     /locus_tag="P5179_11630"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRS95911.1"
                     /translation="MRAHNLFWGAAIVVLAVATMIAFVWKGWHSRQLALRLEQLRAER
                     RELLDEQDKLRARATALSHAARIKDIVSERLGLVEPTTPPIDVPAIGSLGSPDSLAEC
                     RATGTEILRAMRSRSSSGQ"
     gene            2235..>4282
                     /locus_tag="P5179_11635"
     CDS             2235..>4282
                     /locus_tag="P5179_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS95912.1"
                     /translation="MPTTQHEPGLERDVALEASAVRRRVPPPDTDHKRPGLPFPGELR
                     TAPVEANPPRPDFVTAPGQTPPLEPKYRPYDGPAQRAHTIRLVIVGILWLLGLGAITS
                     RLVKIQILDHEKYLAKAAGQQERQITLAARRGKITDRTGRDLAVSLAAASVSVNPRKL
                     TEMKLLSERVSEVSGEPAQRIYQRISKPGRAFAWVARNIETDQAQRIFALAPHAMTIH
                     PVTKRKHPLGGLAGQVLGHVSIDNVGGEGLEALYNQQLRGKDGWMISLVDGLGEQVPN
                     TAQPLMPPVHGNDLTLTIDADYQAIVEQELSKMVDSLHAAGGLAVLYEASTGAIRAMA
                     NVPTYNPDESSQTRNGALRRNRAITDPYEPGSMFKAITVSAAMAEGKVTPDDMIDCQG
                     GRISVAGGMIRDSHPNGVLDVRGVIAHSSNVGTIKISQRISSETFYGYMRAFGLGSVT
                     NVDLPGETRGLLARPSRWSARTHATIAMGQEVGVTALQMAVAYGAIVNGGVLMAPRVV
                     ERITTPDGKIIHAFEPRKVRQVIPAAVADSMVNMLCGVVEKGTGKKAAIPGVRVGGKT
                     GTAQMAAAGRRGYERGAYIASFIGFLPDMDPRLVCVVSVTKPQGSYYGATVAGPVFKN
                     IMTRIINRDAAVLQRPSDAVYPRMPDVVGMSEIGATARLASLGIPVRRSGRGSVVVSQ
                     "
BASE COUNT          927 a         1224 c         1320 g          811 t
ORIGIN      
        1 ggattgctgc gctggagcgc gatattgccc gtcttcaggc ggaccagaaa agaacctcct
       61 cctgattcct cgatcactgg ccgccgctcc ctccccccgc acctcaaaca tcgatcctgc
      121 cgatcgtccc ctcctgccga agcaaggaat ccaaaaaatc tcttgagcgt gtaatacgtt
      181 gttttttaaa gattaaaaac agaacactcc ttcctgaggc aaaaaaaaac tccttagcat
      241 cttgacggaa actatttgcg tgcatatcct agtggtgagc aggggcattt aggggagaat
      301 tggctgaatt cggttcccat aaggggcgcg cacatccggc aacccgaact gccccacccc
      361 tgtgaacagg acaaactcat ggcaaccggt ccactcggag ttgttcggcg acaggccagc
      421 ttcgtcggca actaccgtca cgtcgtggat gcgcagggac ggatccaggt ccctcgtgcc
      481 atccgcaaag ccatgcttcc tgagtcgcag gggactcttt acgtcacgcg cgggttggac
      541 gggtgccttt tcgcgtatcc tctcgacacg tggcaaaatc tcgcggagca gtgggagctc
      601 gcgcttaacg atgcacggtc gtttgagaag cgcaacgaaa tccgcgtaat gacgtctcac
      661 gcgtgcgaag ccaggctgga tggtcaaggt cgcatttcca tcccggcgta cctgctgaaa
      721 gccgttgaca ttagatcgga agcgttattc gtcggcgtca cctatcggat cgaaatctgg
      781 aaccctgatc gctacgaagc ggcgatagca gccgctgaaa actcttttga acagaatatg
      841 cggaacctcg tctcgttccg tcagaaccga cagagcggtt gatccggatg ccggaacatt
      901 atcggtggca cgtgccggtg ctggcagacc aggcggtcga ggcacttgtc tggcgaaagg
      961 acggtaccta cgtcgatgca accgtaggtg acggcgggca tgcagagaag atttgtgagc
     1021 ggctggccgg aagcggtcgg cttctggcca tagacaggga tcaggaggct ctcgaacgcg
     1081 cggcggaccg cctcaaccag tataaagacc gcgtcgtttt cgcgcgggca tcctttgcgc
     1141 ggctcgatga actcgctgaa aatgcgaacc tgggacccat ttccgggatg ctgtttgacc
     1201 tgggagtgtc gtcgcgacaa ctcgaccaac cggaacgcgg gttcagctat cggtcggaag
     1261 gacccataga tatgcgtatg ggacctgacg cggaaactgc ggcggccacc attgtgaacg
     1321 agtggccaga accggagctt gcgcgtctct ttcgcgtttt cggcgaggag ccgcacgccg
     1381 catccatcgc tcgatcgatc tgccagcgga ggaaccgtcg gcctttggca accacctcgg
     1441 acctcgtgga ggcgataggc cggcgaggaa cacatccgga aaagacactt tcgcgcgtgt
     1501 ttcaggcgct cagaatcgcg gtgaacgacg agctggcttc attgaaccgg gtactgggac
     1561 ttgtgccgga cctgcttgaa tgtggcggaa gactcgtggt gatctcgtat cactcactcg
     1621 aagacaggtc ggtaaaggca tggatgcgaa tggaaacccg aggttgcatt tgtccgccga
     1681 ccgtgcctga atgccggtgc ggacacgttc cccggatgag ggccattaca cgccgggcag
     1741 tcatgccgac ggcagaagaa atcgcgggga attcgcgttc gagaagcgcg cgtatgcgcg
     1801 tggcggagcg attgtgagag ctcacaatct gttctggggc gcagccatcg tggtgctggc
     1861 cgtcgcgacg atgatcgcat tcgtgtggaa aggttggcac tcacgccaac ttgccctgcg
     1921 cctggagcaa ttgcgcgcgg aacggaggga gttgctggat gaacaggaca agctgcgcgc
     1981 ccgcgcgact gccctctctc acgcggcaag gatcaaggac atcgtgagcg aacggttggg
     2041 gctcgtggag cccacgacgc caccgatcga tgtgcctgca atcgggtcgc tcgggtctcc
     2101 ggactcgttg gcggagtgca gggcaacagg aacagagatc ttgcgtgcga tgcgctctcg
     2161 atcgtcgtcg gggcaataaa ccgcggctgc agtagggcac acaatgcttt tcgggatacc
     2221 acaaggcgga accgatgcca acaacgcaac atgaacctgg tctggaacgg gatgttgcac
     2281 tcgaggcgtc ggccgtccgt cggcgcgttc cgccgccgga taccgaccat aaacggccgg
     2341 gtctgccgtt tcccggagag ctgagaaccg caccggtgga ggcgaatccc cctcgccccg
     2401 atttcgtaac cgctcccgga caaaccccgc ctctcgaacc gaaatacaga ccgtacgacg
     2461 gccctgcaca gagggctcac accatccggc tggtgattgt cggaattctg tggctcctcg
     2521 gattgggggc gatcacgagt cgcctcgtca agatccagat tcttgaccac gagaaatacc
     2581 ttgcgaaagc ggccggacaa caagaacggc agatcaccct tgccgcgaga cgagggaaga
     2641 ttaccgatcg aaccgggcgc gaccttgcgg taagtcttgc agcagcttcc gtctccgtga
     2701 acccacgcaa actcacggag atgaaactgc tttcggaaag ggtgtccgaa gtatctggag
     2761 agccggcaca gcgaatatac cagagaatct ccaaacccgg acgagcgttc gcgtgggtag
     2821 cacggaatat tgaaacggat caggcgcagc ggatattcgc gcttgcaccc cacgcgatga
     2881 cgattcaccc agtcaccaaa agaaaacatc cgctcggggg actcgccgga caggttctgg
     2941 ggcacgtttc catagacaat gtcgggggcg agggtctcga ggcgctctac aaccagcagt
     3001 tgcgcgggaa agacgggtgg atgatttcgc ttgtggacgg gcttggagaa caagtcccga
     3061 acacggccca gccgcttatg ccacctgtgc acggaaacga cctcacgctc accattgacg
     3121 ccgactacca ggccattgta gagcaagagc tttccaaaat ggtggattcc ttgcatgctg
     3181 ccggagggtt ggcggtcctt tacgaagcat caaccggcgc gatccgagca atggcaaacg
     3241 tgcctaccta caatcccgat gagtcctccc agaccaggaa cggagcgctt cggcggaacc
     3301 gtgccatcac ggacccgtac gaacccggtt ccatgttcaa ggcgataacc gtgtctgcgg
     3361 cgatggccga gggaaaggtc acaccggacg acatgatcga ttgccagggg ggacgtataa
     3421 gtgtcgccgg agggatgatt cgcgatagcc accccaacgg cgttctggac gtccggggtg
     3481 tcatcgcaca ctcttcgaac gtaggaacga taaagatttc ccagcgcata tcgtccgaga
     3541 cgttttatgg gtacatgcgt gcgttcgggc tcggttccgt gacgaacgtg gacctccccg
     3601 gtgagacacg gggattactt gcgcggccgt cgcgctggtc agcgcggaca catgcgacaa
     3661 tcgcaatggg tcaggaggtg ggcgttaccg cgctgcaaat ggccgtggcg tacggtgcga
     3721 tagtgaacgg cggagtactc atggcgcccc gcgtggtcga aagaataacg actcccgatg
     3781 ggaaaatcat ccacgctttt gaacccagaa aggtgagaca ggtcattccg gcggcagtag
     3841 cggattccat ggtgaacatg ctctgcggcg tggttgaaaa aggtaccggg aagaaagccg
     3901 cgatccccgg cgtacgcgtc ggaggtaaga ccgggaccgc gcagatggcg gcggccgggc
     3961 ggcgtgggta cgagcggggc gcctacatcg cctcgttcat cgggttcctc ccggacatgg
     4021 acccgcgtct cgtctgtgtg gtttctgtca cgaagcccca gggtagctat tacggggcca
     4081 cggtcgccgg acccgtgttc aaaaacatca tgacccggat catcaaccgc gacgcggctg
     4141 ttttgcaaag accttctgat gccgtctacc cacgaatgcc agatgttgtc ggcatgtcag
     4201 aaatcggcgc tactgcccga ttggcgtcgc ttggaattcc ggttcgccgt tccggtcgtg
     4261 gttccgtggt agtgtcgcaa tg
//
LOCUS       DAOTNG010000273         4232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_16964_length_4232_cov_5.314580, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000273 DAOTNG010000000
VERSION     DAOTNG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16964_length_4232_cov_5.314580"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="P5179_11640"
     CDS             complement(<1..1763)
                     /locus_tag="P5179_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS95913.1"
                     /translation="MPTLGRDEEVIQGAYGIDLSPRVERLRDYVLNDATWADHYAPRA
                     WLVAESMKQTEGQPPLFRQAAALAHVIRNYPVTIREGELILGYQQHESFRGIGWLEFD
                     NLTNGERREALRNHVQNLSIPDEWKQKVFDAIDYWREHPRVIPHFWPQTPDYISRDTR
                     AGVYFGGGGSEGHTVPGYERVLRHGFHAILEQIDGEIARLQPWKPDDQKRKLWLHSVR
                     LIAAAACELGMRYAEELRALATRESDPLQAEEYRRLADICERVPARGARTFHEAVQSL
                     WFCHLIMGWEDGINANSFGRYDQYLYPFYAADREEGRITEDEALELLEAFHLALYKSY
                     DVQQATIGGQNPDGTDATNELTYLFLEAMWRVNLIRCLSVRVHRKTPRRLLESAFRLI
                     RRGGGVPFFFNDEAIVPALVEKGIPVEDARQYAIIGCVEVTIPGKTNPHAVSHMMNVA
                     KCLELALNDGKDLATGEQIGPQTGTLADMPDIEALWNAYKLQLEHFARHAVELSNQGD
                     LAQPFRFPLPYTSILTDDCIARGKDMTDGGAHYHYHSTCAIGLPNVGDSLYAVDQLVF
                     KEKSVSPDELMQALKANWEGE"
     gene            complement(1793..2761)
                     /locus_tag="P5179_11645"
     CDS             complement(1793..2761)
                     /locus_tag="P5179_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRS95914.1"
                     /translation="MHSPEHPHKSDSQIPSTAVKGRIFDVQTFSIHDGPGIRTTVFLK
                     GCPLRCVWCHNPESMETEPDVGFIPAKCIACGQCTLVCTHEGHEIVDGRHVFQRGLCV
                     RCGSCTTECPTGALELAGKEVTAAWVMSEVEKDRMFYERSGGGITLSGGEPLMQPEFS
                     AALLRLARDSRLHTAVDTSGYAGHTVFDRVTALADLVLFDIKMIDERAHRRLTGVSNR
                     RILRNLQRLLRDENGPKVWLRLPVIPGQNDSPDAIQNLIDFCASLWGNPRLEAIHLMP
                     YHRLAESKYQEFGKRYLINGTHPPADEDISAIIRRFSERGVPVTRS"
     gene            2904..4001
                     /locus_tag="P5179_11650"
     CDS             2904..4001
                     /locus_tag="P5179_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95915.1"
                     /translation="MTDRERVLAIMHHQPVDGVVWQPRIDHWYYTNKRAGTLPERYRD
                     MSLYEVYVDLDCSIRGYEFYNPCISRVYDSSVSFRVVEDTPHRRVRLWETPLGVLTEV
                     EGKTAESSLLTEFPAKSIADLRIVRYTLEALNFEFSEERFWEAEKLVGDRGVGSVYNL
                     RTPLMRLFIEFMGFENTIVALHEFPEEMDSFIRFLEEDDQKYIDVIKKSPLPLLNFGD
                     NVDSNMLPPSLMRKYALPYYRRRTAELRAAGKASYPHWDGALKGLLPFVRECGFDGYE
                     AFTPTPMGDVSLDEIAEAMGHDYVLIDGIPATHFIPPFTRDDVEEFATLVLQTFAPSL
                     ILGISDELPPSGDIESVRRVAEIVRSFRISK"
     gene            4038..>4232
                     /locus_tag="P5179_11655"
     CDS             4038..>4232
                     /locus_tag="P5179_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95916.1"
                     /translation="MTGVSSPQITTHDRQVLRDLAKEILDIAHSDENLEKKGAWYAHN
                     DLKPRRPLVLIESFPATDELV"
BASE COUNT          851 a         1193 c         1249 g          939 t
ORIGIN      
        1 tcctcccctt cccagttcgc cttgagcgct tgcatcaatt cgtcggggga gacgcttttc
       61 tccttgaaga cgagctggtc caccgcgtac agggagtcac cgacgttggg caagcctatc
      121 gcgcaggtag aatggtaatg gtaatgcgcc ccgccgtcgg tcatatcctt cccgcgcgct
      181 atgcaatcgt cggtcagaat ggaggtgtag ggcagcggaa agcggaaagg ttgcgcgagg
      241 tcgccctggt tcgaaagctc caccgcgtgc cgggcgaaat gctcgagctg aagtttgtag
      301 gcgttccaga gtgcttcgat gtccggcatg tccgcaagag tccccgtctg cggtccgatc
      361 tgttcgccgg ttgcgaggtc cttgccgtcg ttcagcgcca gctccaggca cttggccacg
      421 ttcatcatgt ggcttaccgc gtgggggttc gttttccccg gaatcgtcac ctccacgcag
      481 cctatgatgg cgtactggcg ggcatcctca acaggaatcc ccttctccac aagggccgga
      541 acaatcgctt cgtcgttgaa gaagaacggc acgcctccgc cccgcctgat caaccggaat
      601 gcggattcga ggagccttcg cggggtcttt cggtgtactc ttaccgaaag gcagcgaatg
      661 aggttcactc gccacatcgc ctcgaggaac aggtacgtca gttcgttggt cgcatccgtg
      721 ccatccgggt tttgtccgcc gatggtggcc tgctggacgt cgtagctctt gtagagtgca
      781 agatggaagg cttcgagcag ttccagcgct tcgtcttctg tgattcgccc ctcttccctg
      841 tcggcggcgt agaacggata gaggtactgg tcgtaacggc cgaaagagtt tgcgttaatc
      901 ccgtcttccc atcccatgat cagatggcag aaccagagtg attgaactgc ttcgtgaaac
      961 gtccgcgcgc cgcgggcggg tactctctca cagatgtcgg caagacgacg gtactcttcc
     1021 gcctgaagag ggtcgctttc gcgcgtggcc agggctcgca gctcctcggc ataccgcatt
     1081 ccgagttcgc acgccgctgc cgcgataagc ctcacgctgt ggagccacag ttttcttttc
     1141 tgatcgtccg gcttccacgg ttggagtctg gcgatctcgc cgtctatctg ttcaaggatc
     1201 gcatggaagc cgtgccgcaa aacccgttcg tatcccggaa cggtatgccc ttcgctccca
     1261 ccgccgccga aatagacgcc tgcccgcgtg tcacgggata tgtaatcggg tgtttgtggc
     1321 cagaaatgag ggattacgcg agggtgttcg cgccagtagt cgattgcgtc gaaaaccttt
     1381 tgtttccact cgtccgggat actcagattc tggacgtgat tacggagcgc ttctctgcgt
     1441 tcgccgttgg tgagattatc aaattccagc cacccgattc cccggaagga ttcgtgctgc
     1501 tgatacccca ggatcaattc gccttcgcgg atcgttacgg ggtagttcct gataacatgt
     1561 gccagtgccg ccgcctggcg gaacagcggc ggttgcccct cggtctgctt catgctttcg
     1621 gcaaccagcc acgcccgcgg ggcgtaatgg tcagcccatg tcgcgtcgtt gagaacgtaa
     1681 tcgcgcaacc gttccacgcg cggtgaaagg tcaattccgt acgcgccctg aattacttct
     1741 tcgtcacggc cgagggtggg cataggagca tgcatctccg ttgtggaagg cgctaactgc
     1801 gcgttaccgg cactccgcgt tcggagaaac ggcgtatgat tgccgatatg tcttcatcag
     1861 caggcgggtg cgttccatta ataagatacc gttttccgaa ttcctggtat ttgctttccg
     1921 caagtctgtg gtagggcatc aggtgaattg cttcaaggcg gggattaccc cagagcgacg
     1981 cacagaaatc gatcaggttc tggatggcgt cgggcgagtc gttctgcccg gggatgacag
     2041 ggagccgcag ccatactttg gggccgtttt cgtcccgaag aaggcgttgc agatttcgca
     2101 gaatgcgccg gttcgacacg ccggtgagtc gccggtgtgc gcgttcgtct atcattttta
     2161 tgtcgaaaag gaccagatcc gcgagggctg tcactcgatc gaacaccgta tggcccgcat
     2221 aaccggacgt atccactgcc gtgtggaggc gggaatcgcg agccaggcga agcagcgcgg
     2281 cggaaaactc cggttgcatg agcggctcgc cgcccgacag ggttatcccg ccgccggagc
     2341 gctcgtagaa catgcggtcc ttctccactt cggacatcac ccacgcggca gtgacctctt
     2401 tgcccgcaag ttcaagcgca ccggttgggc attccgtcgt gcacgagccg caccgtacgc
     2461 agagaccccg ctggaacacg tgcctgccgt cgacgatctc gtggccctca tgcgtgcaca
     2521 cgagcgtgca ctgaccgcat gcgatgcatt tcgccggaat gaaacccacg tcgggttcgg
     2581 tttccatgga ttcggggtta tggcaccata cgcatcgaag ggggcagcct ttcagaaaca
     2641 cggtcgtgcg gattcccgga ccgtcgtgga tcgaaaacgt ctgtacgtcg aagatcctac
     2701 ctttgacggc agtgctcggg atctgagagt cacttttgtg gggatgttcg ggagagtgca
     2761 tgggtaccgc gtgtgagtgt gattgagaac cggctccatc aaatatagcg aagcgttcga
     2821 aaagagcgaa cccttggacc aaacgccatt ttcgttagag tacgtacgga aggtttccgc
     2881 gccgatggat tttggagggg aacgtgacag atagagaacg agttctcgca atcatgcatc
     2941 accaaccggt ggacggagta gtgtggcaac cgcgcataga ccactggtac tatacgaata
     3001 agcgcgcagg aacgctgcca gaacgatacc gggacatgtc tctgtacgag gtgtacgtcg
     3061 acctcgattg ctccatccgg ggatacgagt tctacaaccc gtgtatcagc cgtgtgtacg
     3121 attcctcggt gtccttcagg gttgtcgaag acactccaca ccgtcgcgtc cgtctgtggg
     3181 aaaccccgct tggagtgctc accgaggtgg aggggaagac ggcggaatcg agccttctta
     3241 ccgagtttcc ggcgaaatcg attgctgacc tccggattgt tcggtatacg ctggaagcgc
     3301 tgaatttcga gttcagtgaa gaacggttct gggaagccga gaaactggtc ggggacagag
     3361 gagtcggttc ggtgtacaac ctccgtactc ccctcatgag gctgttcatc gaattcatgg
     3421 gatttgagaa caccatcgtt gccctccatg agttcccgga ggaaatggac tcgttcattc
     3481 gtttccttga agaagacgac cagaagtaca tagacgtgat caagaaaagc cctctgccgt
     3541 tgctcaattt cggagacaac gtggattcga acatgctgcc gccgtcactc atgaggaaat
     3601 acgcgcttcc ctattacagg cgtcgaaccg cggaactccg tgcggcgggg aaggcctcgt
     3661 accctcactg ggacggtgcg ctcaaaggtc tgcttccttt tgtgcgagag tgcggatttg
     3721 acgggtacga agcgttcacc cccaccccga tgggtgacgt gtcgcttgat gagatcgcgg
     3781 aagcgatggg gcacgactac gttcttattg acgggatccc cgcaacacat ttcatcccgc
     3841 ctttcacccg ggatgacgtt gaggaattcg cgacgcttgt tcttcagacg ttcgctccga
     3901 gtctcatact cggtatttct gacgaattgc ccccctcagg ggatattgag agcgtacggc
     3961 gcgtcgcgga gattgtgcgc agttttcgta tttccaaata attcagacca accgccgcac
     4021 actacgaaga ggccgagatg acaggcgtat cctcaccgca gattacgacg cacgatcggc
     4081 aggtgctccg cgatctcgca aaggaaatcc ttgatatcgc gcacagcgat gaaaaccttg
     4141 aaaagaaagg cgcgtggtac gcccacaacg acctgaaacc tcggcggccg ctggtgttga
     4201 tagaatcgtt tccggcaacg gatgaactgg ta
//
LOCUS       DAOTNG010000274         4226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17001_length_4226_cov_5.275234, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000274 DAOTNG010000000
VERSION     DAOTNG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17001_length_4226_cov_5.275234"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1460
                     /locus_tag="P5179_11660"
     CDS             282..1460
                     /locus_tag="P5179_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95917.1"
                     /translation="MHRPLSDFGVTPGGDPVKNAARLQEGIDWASPRGGALYVEPTDE
                     PYRVAGGVILRQNVSLIGVHGPVGRGTRHPERKQPVGSVFAIEDEERPFLTVEHATQV
                     RGIQFWYPRQTADDASRIIPYPATIRVSPDTGAQGVTLSCLTFFGEYLAMDFAASPKC
                     ICEQILFEHCYGYPLGGTFIRIDYCYDIPRILHCHVNPANRRFIDGGHGKAVIDATMA
                     RKTFCYSIDHTDNAQLMDLFTFGTWGGIHLGPATYGQLTNFNLDCVAVGILKQGDQNF
                     NRNWQIAQGSIIANGGAPLEDIHPLIVEGQGHTALANVESFSGHNGALTTLGQSQDFL
                     LLRGNERLTVSMFGCRMRNYAAAEPVTVQNPKAVVQAVACVDHHENPWPAKPFCARGE
                     "
     gene            2083..3972
                     /locus_tag="P5179_11665"
     CDS             2083..3972
                     /locus_tag="P5179_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95918.1"
                     /translation="MPSSMNATGYTPQRTGTTIYVSRLGDNSDGRSWTTAFTTVQAGL
                     DAIPDSLGGHRILVRPDTYMEANLHPAHPGAEGSYNLFDVDFDGSLGSGAAGYAVLDA
                     SDPKKGMQSIDYWQVPRSSVEYPGVEWDRWIVRHVYATGGDAGLFWDNDTTPFSVIVE
                     DSVGIGRAFGGGAGNVLPREGEPMIWRRCCLWSLDWWGDTAGAYCRAENPDPREEPDF
                     IFEDCTLVGPQCALKSGNPGFSTYSHIRVERCRLIVLNFSQPRGTPSDGIIQSVIDGK
                     YLHVDLEDTTLMGYKVFGVRQNKETVDQIGYTTKGCVQAYVQFEQEVPKGIQPMGHWP
                     ADVFEFIKPPAPPAPAVPSARRPVLRDPESVENHVCELTPFVWKGRLCHMTCVRPVDA
                     DPDRGLYLRLSDAETGAELARFAEGYSLASAIVWNDTFYAFASRHGDGTWNDVTLFKS
                     SDLRNWTQKVVIEQEGAEHLFNTSVCATPGGFVMAYESDDPAYVPFTVKYAESADLEN
                     WTKVSDAVFGPDRYAACPCVRYSDGWFYQMYLEHRTPRWFFETHIARSKDLKTWHLSP
                     MNPVITPEGLDEGNNTSDPEIVEFAGKTYLYYAVGDQLTWTRLKRKTYDGPVARFFAA
                     WWAGS"
BASE COUNT          798 a         1345 c         1368 g          715 t
ORIGIN      
        1 gatagctcag cccggggcag cgtcccgggc gtaggaaaac atggccgaga cctccctccc
       61 gccggcgtgc ccctgtaccg gcgggtggtt ctcggatcgt tgagtgaaat gctgcgtagc
      121 acgggcatct tgcccgtgca cggtataacg ccgctttgcg ttctggttcg ggcaagatgc
      181 ccgcgctacg gctggaatga ggctaggtta ccggcgcgga ttgatgcaac aaggaatctc
      241 cgcccgcgta tggctccccg caatcaccga aaggaacaac cgtgcatcgc ccactgagcg
      301 acttcggcgt cacccccggc ggtgatccgg tcaaaaacgc cgcgcggctg caagaaggca
      361 ttgactgggc atcgccccgc ggcggcgcgc tttacgtgga accgacggac gaaccgtacc
      421 gggtcgccgg aggggtaatt ctccggcaga acgtgtccct catcggtgtg cacggcccgg
      481 tggggcgagg aacgcggcac cccgagagga agcaaccggt cggcagtgtg ttcgctatcg
      541 aggacgagga gcgccccttc ctcaccgtcg agcacgcgac acaggtgcgc ggcatccagt
      601 tctggtatcc ccgccaaaca gccgacgacg ccagccgcat catcccctac cccgccacca
      661 tccgcgtctc gccggacacc ggcgcgcagg gggtgacgct ctcctgcctc acgttcttcg
      721 gcgagtatct ggcgatggat ttcgctgcca gcccgaaatg catctgcgag cagatcctgt
      781 tcgagcactg ctacggctac ccgttgggcg gcacgttcat ccgcatcgac tactgctacg
      841 acatcccgcg tattctgcac tgtcacgtca accccgcgaa ccgccggttc atcgacggcg
      901 gccacggcaa ggcggtgatc gacgcaacca tggcgcggaa aacgttctgc tactccatcg
      961 accacacaga caacgcccag ctcatggacc tgttcacgtt cggaacgtgg ggtggcattc
     1021 accttggccc ggcaacctac ggtcagctca cgaattttaa cctggactgc gtggcggtgg
     1081 gcattctcaa gcagggagat cagaacttca accgcaactg gcaaattgcg cagggctcca
     1141 tcatcgcaaa cggaggcgcg ccgctggagg acatccatcc gctcatcgtg gagggacagg
     1201 ggcacaccgc gctcgcgaac gtcgagtcat tctccggcca caacggcgcg ctgacgaccc
     1261 tcgggcagtc gcaggatttc ctgctcctgc ggggcaacga acggctgacg gtgagcatgt
     1321 tcggctgccg catgcgcaac tacgccgccg cggagccggt gaccgtgcaa aacccgaagg
     1381 cggtcgttca ggcggttgcc tgcgtggatc accatgagaa cccctggccg gcgaagccat
     1441 tttgtgcacg cggggagtga tgcgccacgg cgcgttcggc gaacgcaccc tgcctttttg
     1501 gagggacccg ctctgtcggg tcccaaacac ggcggcggca gcccgcccca ccggtgaaag
     1561 cgcggcgcat ttcgtgcccg gccctgcgag ccgggctgag atatttcgcg tcgacggcgc
     1621 cggtgcgtct cgatacagcg aaccggtcgc caaacttcgg gaaccgcagg agacctgttg
     1681 aagcgccggt ttgtttctca gcgaccggtt cgccactcga cgaccggggt cgttgcacga
     1741 ctaccagcac tcactccggt agtcattccg cgcgctcaat cgagcacgac gcgaaggcgg
     1801 gaatccagaa gaggcatctg cggcggtctc ggcgagaccg ccctaccctg caaaccgggc
     1861 gacgggggca aaaggccccg aatgggcggg tccctgagcc tgtcgaaggg cacgcccggg
     1921 aaatgcgttg gcgggagcac gctcagggcc cgcgcaggat gggcgctcgc cggtgcgagc
     1981 gcctgaggaa ggcctgaagg gccgatatag ctcagcccgg ggcaacgtcc cgggcactac
     2041 agcggaagaa ccggattaca gccctaggaa agggaagaca aaatgccatc gagcatgaac
     2101 gcaacgggat acacccctca acgcacaggc acgaccatct acgtctcccg gctcggggat
     2161 aactccgacg ggcgatcgtg gaccaccgca ttcaccacgg tgcaggccgg tctggacgcc
     2221 atacccgaca gcctgggcgg ccaccggatt cttgtccggc ccgacacgta catggaggcg
     2281 aatctgcacc ctgcgcatcc gggagcggag ggttcctaca accttttcga cgtggacttc
     2341 gacggctcgc tgggttccgg cgccgcagga tacgcggtgc tggacgccag cgatccaaaa
     2401 aagggcatgc agagcattga ttactggcag gtgccgcgca gctcggtgga gtaccccggc
     2461 gtcgagtggg atcgctggat cgtccgccac gtctacgcga cggggggcga tgcgggcctg
     2521 ttctgggata acgacacgac tccgttctcc gttatcgtgg aagacagcgt cggcatcggc
     2581 agggcgttcg gcggcggcgc gggcaatgtg ctcccccgtg agggcgagcc gatgatctgg
     2641 cggcggtgct gcctctggtc gctggactgg tggggcgaca ccgccggggc gtattgccgc
     2701 gcggagaacc ccgacccgcg cgaggagccg gatttcatct tcgaggattg cacgctcgtc
     2761 gggccgcagt gcgcgctgaa atcgggaaac cccggtttca gcacctattc gcacatcagg
     2821 gtggagcgct gccgcctcat cgtgctgaat ttctcgcagc cgcgcggcac cccgtccgac
     2881 ggcatcatcc agagcgtgat cgacgggaaa tacctccacg tggatctgga agacaccacc
     2941 ctgatgggct acaaggtgtt cggcgtgcgc cagaacaagg agaccgttga ccagatcggc
     3001 tacaccacga agggttgcgt gcaggcctat gtccagttcg agcaggaagt gccgaagggc
     3061 attcaaccca tgggccactg gcctgccgac gtgttcgagt tcatcaaacc acctgcaccg
     3121 cccgctcccg ctgtcccgtc agcccgtcgc ccggttctcc gagacccgga atccgtggaa
     3181 aaccacgtct gcgagctgac cccttttgtg tggaaggggc ggctctgcca catgacgtgc
     3241 gtgcgcccgg tggatgccga tcccgaccgg ggtctctatc tccggctctc ggatgcggaa
     3301 acgggcgccg aactcgcacg cttcgccgag ggatacagcc tcgcgtcggc gatcgtctgg
     3361 aacgacacct tctacgcgtt tgcctcccgg cacggcgacg gcacatggaa cgacgtcacc
     3421 ctgttcaagt cgtccgacct gaggaattgg acgcagaagg tcgtgatcga gcaggagggc
     3481 gcggagcatc tgttcaacac gtccgtctgc gcaacgccgg gcgggtttgt gatggcctac
     3541 gaatccgacg atcctgcgta cgttccgttc acggtgaaat acgccgaatc agccgacctt
     3601 gagaactgga cgaaggtgtc ggatgccgtg ttcgggcccg accgctatgc cgcatgcccg
     3661 tgtgtccggt attcagatgg gtggttctac cagatgtatc tggagcacag aacgccgcgc
     3721 tggtttttcg agacgcacat tgcacggtcg aaagacctca aaacgtggca tctgagcccg
     3781 atgaaccccg tgatcacccc ggagggtctc gacgagggca acaacacgtc cgatccagaa
     3841 attgtggaat ttgcggggaa aacctacctc tactacgccg tcggcgacca gctcacatgg
     3901 acgaggctca agcgcaagac gtatgacggc cccgtggcgc gtttctttgc ggcgtggtgg
     3961 gcgggttcgt aggcacgggt gggccgggga accgtagggc gcgacgcatg cgtcgctacc
     4021 cacaaatgcc catggtaggg cgcgttgtcc tgcgccgccg tgggcgacgg tgaacaaaga
     4081 cgcacgcgaa gggccagggg caacgtaggg gcggaccggt gtgtccgccc gcgttggcac
     4141 acaattcgaa cacgggcgga cacaccggtc cgccccttgt atcttggtag tgttctggtg
     4201 aggatgtttt tggggttcgg tagatc
//
LOCUS       DAOTNG010000275         4224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17010_length_4224_cov_3.393140, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000275 DAOTNG010000000
VERSION     DAOTNG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17010_length_4224_cov_3.393140"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5179_11670"
     CDS             complement(<1..421)
                     /locus_tag="P5179_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS95919.1"
                     /translation="MAHATLLQLVAILVLGILAQWIAWRIRIPSIVLLLAIGILSGPV
                     WGWLNPDNLLGDLLLPTVSLSVAVILYEGGLNLRFRELQNIGGVFLRMITLGVAISWV
                     VGAAAARYVLGFPWAIATLLGAILVVTGPTVIGPILRH"
     gene            complement(629..2260)
                     /gene="clcA"
                     /locus_tag="P5179_11675"
     CDS             complement(629..2260)
                     /gene="clcA"
                     /locus_tag="P5179_11675"
                     /inference="COORDINATES: protein motif:HMM:NF003640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HRS95920.1"
                     /translation="MKDAAHNQDAPGGTATTASVRSEIQEYLDIRQQRRWIFPRAALV
                     GVFAGIAALLFRAALTGADVFRNGLIAWAQGMPLLGWVFPVGFAVLGAALSVAMTRRY
                     APEASGSGIPHLEAVLRRFRKLEWKRVLPVKFFGGLLAIGSGLALGREGPTVQMGGAV
                     GDAVSRWLKVSEKERLTLISAGAAAGLSAAFNAPLSGLMFVLEEVRRDFHPVVFGAAF
                     LAAAIADIIARIGLGQFSVFAVPSYPLPPLSSLPIFAVVGIAAGGLGVLFNRGLLTAV
                     TAFRRVPPRFVFPVVAATGGAAGLAGWFSPVMIGNGHALAEVALNGRMLLAAIPLFFV
                     VRFLLTVTSYGIGAPGGIFAPLLVLGAFIGLAVGQIAHHFSPDIAPIPAAFAVVGMAA
                     YFTAIVRAPLTGIVLIIEMTENYSLMLPLLISCFCAYAVAELLKDVPVYEALLEEDLK
                     RTGDAYHLAEPTVAEFTIEEGAPFDGRLVRSLGLPAGCIIVRCWDGRREWVPKANTRL
                     EAHMRIMAVISPDAADALDKLREGCEEVEGEKNRG"
     gene            complement(2530..>4224)
                     /locus_tag="P5179_11680"
     CDS             complement(2530..>4224)
                     /locus_tag="P5179_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS95921.1"
                     /translation="NHCRTSPVYREKCQIINTRLATRYKDHPALVVWHVSNEYNGGDC
                     HCPLCYEAFHTWLKKRYGTLEELNHAWWTGFWSHAFTDWAQIRPVDPSIHGLMLDWQR
                     FKTDQTIDFFRNELAPLREFTPDIPVTTNFMGFSPTLDYAKFAREVDVVSWDSYPAYH
                     DRPNDWLNAVEVSLTHDLNRSFKNKPFMLMECSPSVQNWKPVNKLKRPGLHRVEALQC
                     IAHGGDTVQYFQWRKGRGGCEKFHGAVVDHYPTEHTRVFREVADLGGVLASLDDVVGT
                     ATPAEVAILYDWENRWAIDQTAGPRNERKDYQPTCVAHYRPFWSAGVSCDVVNEDADF
                     SGYRLLIAPMLYMIRPGVAERIKDFVANGGVFVTTYLSGIVNESDLCFQNGFPGPLRD
                     LLGIWVEEIDALYDNETVQVVAEPGNGAGLSGTYAAGIFCDVVHAEGAQVLATYGSEF
                     YRGHPAVTVNHRGKGRAYYIASRNEHRFHADFARHLIDDLGLRRALGTELPDGVTAQI
                     RTDGEREYVFVLGFNRETVTIELGGARYRDRLTGERVSGSMSLQPYTVRILEPVTE"
BASE COUNT          873 a         1337 c         1240 g          774 t
ORIGIN      
        1 gatggcgcaa aattgggccg atcaccgttg gaccggttac cacgaggatc gcgccgagga
       61 gcgtggcgat cgcccacggg aagccaagga cgtaccgtgc ggccgcggct ccaacgaccc
      121 atgagattgc cacgccgaga gtgatcatgc gaaggaagac ccctccgatg ttctgcaact
      181 cccggaacct gaggttcagt ccgccttcgt acaagatcac tgcgacggag agtgacacag
      241 tgggcagcaa gaggtcgccc aaaaggttat ccgggttgag ccatccccag accggacctg
      301 aaagaatccc aatagccagc aagagcacga ttgagggaat ccggatgcgc caggcaatcc
      361 actgggccag aatgccaagg acaagtatcg caaccagttg gagtaacgtc gcatgcgcca
      421 aacgaatgcc ctcactgcaa acgacaggga tgctgctccg acaaaagcac aggatagatg
      481 gtacgcacgg ggccttctct gtgcaccgct tcggcttatt tccgcctttc tccgggttgc
      541 cgcccgtccg tttttttcac cccgcccgtg gcaaaaaagc agggcgaact cgcagtcgtg
      601 ttcctccgca aggcggtttt catgccggct agccgcggtt cttttccccc tccacttctt
      661 cacatccctc ccggagcttg tcgagagcgt cggcggcgtc cggggagata actgccatga
      721 tgcgcatgtg agcttcgagc cgggtgtttg ctttcggcac ccattcgcgc ctcccgtccc
      781 agcatcgaac aattatgcag cccgccggca acccgagtga gcgcacgagc cgcccgtcaa
      841 aaggagcgcc ttcctctatg gtgaactccg cgaccgttgg ctcggccaga tgatatgcgt
      901 ccccggtgcg tttgaggtcc tcttccagga gcgcttcgta gactggcaca tctttcagca
      961 attccgcgac ggcgtacgcg cagaagcagc ttatgagaag gggcaacatg agggagtagt
     1021 tctcggtcat ttcgataatc aacacaatac cggtgagcgg agcccgaacg atcgccgtga
     1081 agtacgctgc catgccgaca actgcaaaag ccgccggtat cggcgcgatg tctggagaga
     1141 agtggtgagc gatctgcccg accgccaggc cgatgaatgc tccgaggacg agcaacggcg
     1201 cgaagatgcc tccgggtgcg ccgattccgt aactcgtgac cgtaagaaga aaacgcacca
     1261 caaagaagag agggatcgct gcgagaagca ttctgccatt gagagcaacc tccgccagcg
     1321 catgaccgtt gccgatcatc accggggaga accatcccgc caggcctgct gccccgcccg
     1381 ttgccgcgac aacggggaat acgaaccggg gaggcacccg ccgaaacgcg gtgacggcag
     1441 tcaacaggcc gcgattgaat aacacgccca atccgcctgc cgcaataccc accaccgcga
     1501 aaatcggcaa cgaagaaaga ggaggaaggg gatagctcgg aaccgcgaaa acggaaaact
     1561 gccccagacc tattcgcgcg atgatgtcag cgatcgccgc cgcgagaaat gccgcgccga
     1621 acacgacagg gtggaaatcg cgcctgactt cctcaaggac gaacatgagc ccggacagcg
     1681 gcgcgttgaa cgccgccgaa agccctgcgg ccgcgccggc ggaaatgagg gtaagacgct
     1741 ccttttcaga taccttgagc cagcgggaga ccgcatcacc gacggcacct cccatctgaa
     1801 ccgtcggccc ttcgcgtcca agcgccaggc cgctgcctat tgcgagaagt cccccgaaga
     1861 acttcaccgg cagcacacgc ttccactcca gcttgcgaaa acggcgcaga acggcttcga
     1921 ggtgcgggat accacttcca ctggcttccg gagcgtatcg ccgcgtcatt gcgacggaaa
     1981 gtgccgcccc cagcacggcg aacccgaccg ggaagaccca cccgaggagt ggcatcccct
     2041 gcgcccacgc aatgaggccg tttctgaaca catcagcgcc cgtcagcgcc gccctgaaca
     2101 acaacgccgc gattccggca aaaacgccta ccagagcggc gcgtgggaaa atccagcgcc
     2161 tctgttgccg gatatccagg tattcctgaa tctcagaacg caccgaagcc gtggtcgcgg
     2221 tgcctccagg cgcatcctga ttgtgagcag cgtctttcac gtgaatgagt aactccctaa
     2281 tcccgcgaat gcgcgcggac cctggccaag ctcacatctg tggtgcagtc attgttgctc
     2341 cgacccgggt agtgccgccg gagcacggga cgaagctcag gcagttgccc cgggctcaat
     2401 gcatcgctct ccacacaagc tttcgcacct cttccaccca cagaaccgcc aacgcgaatg
     2461 gtttttgctt tttttgcggt tgccatgggt tgtcctttct tcaactcttc gttgcacccg
     2521 gttagaggat cactcagtca ccggctcgag tattctgacg gtgtaaggct gcagggacat
     2581 ggaacccgac acgcgctcgc ctgtcaggcg gtcgcgatac cgcgcgccgc caagttcgat
     2641 ggtcacggtc tcccggttga aaccgaggac aaagacgtat tcccgctcac cgtccgtccg
     2701 aatctgtgcg gtcacgccgt cgggaagctc cgttcccagc gcccgacgca ggccgaggtc
     2761 gtcaataagg tgccgcgcga aatccgcgtg gaagcgatgt tcgttgcggc tggcgatgta
     2821 gtatgccctg cccttgcccc ggtggttcac cgtaacggcc ggatgccctc tgtagaactc
     2881 gctgccatac gttgccagca cctgagctcc ttccgcatgc accacgtcgc agaaaatgcc
     2941 cgcggcatag gtgccgctga ggccggcgcc attgcccggc tcagcgacca cctgcaccgt
     3001 ttcgttgtcg tacagtgcgt cgatttcctc cacccagatt ccgagcagat cgcggagtgg
     3061 gccgggaaac ccgttctgaa agcacaggtc gctttcgttg acgattcctg agagatacgt
     3121 cgtgacgaac acaccgccgt tcgccacaaa atccttgatc cgctccgcaa ccccggggcg
     3181 gatcatgtag agcatgggcg cgatgagcag cctgtacccg gagaaatccg cgtcctcgtt
     3241 aaccacgtcg caggagacgc ccgcggacca gaagggccgg tagtgcgcaa cgcaggtagg
     3301 ttggtaatcc ttgcgctcgt tccgtggccc cgccgtctgg tcaatggccc agcggttttc
     3361 ccagtcgtag agaatggcaa cctcggccgg ggttgccgtt cccaccacat cgtccagcga
     3421 ggcgaggacg ccgccgaggt ccgccacctc gcggaacacc cgcgtgtgtt ccgtcgggta
     3481 gtggtcaacc accgcgccgt ggaatttctc gcacccgccg cgaccctttc gccactggaa
     3541 gtactgcaca gtgtcgccgc cgtgggcaat gcactggagc gcttccaccc tgtgcagccc
     3601 cggccgtttg agcttgttca ccggcttcca gttctgcaca ctgggtgagc actccatgag
     3661 catgaacggt ttgtttttga aggaacggtt caggtcgtgg gtcagcgaga cctcgaccgc
     3721 gttcagccag tcgttcgggc ggtcgtggta agcagggtaa ctgtcccagc tcacgacatc
     3781 cacctctcgc gcgaacttcg catagtccag cgtcgggctg aatcccatga agttggtggt
     3841 tacggggatg tccggggtga attcgcgcaa aggcgcgagt tcattccgga agaagtcgat
     3901 cgtctggtct gttttgaagc gctgccagtc cagcatgagc ccgtgtatgc tggggtccac
     3961 aggcctgatc tgcgcccaat cggtgaaagc gtggctccag aaacccgtcc accaggcgtg
     4021 attgagttcc tccagggtgc cgtaacgttt tttcagccac gtgtggaagg cttcgtaaca
     4081 gagcgggcag tggcagtccc caccgttgta ttcgttagag acgtgccata cgaccagcgc
     4141 cgggtggtct ttgtagcgcg tggcaagccg ggtattgatg atctggcatt tttcgcgata
     4201 cacgggcgac gtccggcaat ggtt
//
LOCUS       DAOTNG010000276         4207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17092_length_4207_cov_5.211464, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000276 DAOTNG010000000
VERSION     DAOTNG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_4207_cov_5.211464"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="xerD"
                     /locus_tag="P5179_11685"
     CDS             complement(<1..921)
                     /gene="xerD"
                     /locus_tag="P5179_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRS95922.1"
                     /translation="MERKSVKSSPRIARDASRGAESSPAQLPGLRLLSSFLQYITAER
                     GLSQNTQAAYRRDLDRYVRFLETKRGGMTPRTATREDVSSFFSWLRGQNLAPSSIART
                     MSAVRVFHRFLQNEGVAESNPTGTLDTPKLERNLPEVLSRNEIERLLNAPDPVTPLGL
                     RDRAMLECAYATGLRVSELISIDIRNLFFNEGFIRVTGKGNKERLVPIGRKAVECISA
                     YVATARSAVAKPESGNVLFLNWRGRPLTRMAFWQMLKNYCRKTGITKTVSPHTLRHSF
                     ATHLLEGGADLRAVQEMLGHADISTTQIYTH"
     gene            complement(942..2936)
                     /locus_tag="P5179_11690"
     CDS             complement(942..2936)
                     /locus_tag="P5179_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRS95923.1"
                     /translation="MLKRRFTRLYWRIGEVAEITGISQQTLRAWEQELAILRPRRDAS
                     GVRVYRERDFRIVLVLQQLIEKEKWSVSDVKELLVAAPDEIRAMINAVPPEALEGTPD
                     ENEPSTTARTVEKPEVAATPHEDKNEEPAQAGVRLAGAAVPRETEQEVTEAPAEEPGS
                     GGGEVVETPAVGAAAYPPAETEERELAGASSPTLPLTEEPSTVVGAAEAEETAEPASG
                     EMSAEERSFPAEGVSEAEETWQDTGLPPFATETPAEPEREPVEAPTSTEPVVETSDGD
                     MDLAASAPPIEREELAPVEVQDATPSVPVEANLMEQPVLPGDQIATAEDVSPATETGY
                     HPEEEDREQVFAAAEQIESFVHSEGMAEETAGGPAAGEATEEIGMAAPVWTPVEEEGE
                     ASLQGEPEAATPREASLPDSEFEPEPELAESGEKEHLNQPVVPEVAAEESEPSPAVQP
                     WDGAPVTDVAETPASASVPGPEPLPSATAITWEAESEIAAEAAETAAQIAESGFVEAP
                     ENEGADGTRDSIANMAFPQEQPESAPETGAPSDANVASETEPGETDTEEEEAISPLLA
                     MPADTQTELSTHEEIEPPRPERREVFELIASEAVPVPETLPKSVVEDLRALREELAAI
                     AEMVRMLVDPSPESTEATKDVSDGRALAECSSGDEKSSDA"
     gene            complement(2981..3985)
                     /locus_tag="P5179_11695"
     CDS             complement(2981..3985)
                     /locus_tag="P5179_11695"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRS95924.1"
                     /translation="MNVVVMRAGSWGSVLSGLLARNGHRVSLIAPHEALRTELLTYRE
                     NRKALPGVPIPDAVAILSAPDDVLESADMVVFPGPCELMRDMARSVKSANWQGKLVVT
                     AAKGIEQGSFKRMSELLAEELPEVPPDEIVALSGPSFARFVGLGHPTTVVAAARSEKA
                     AVAAQRAFMSSTFRVYCSPDSLGVELGGALKNVIAIAAGMSDGLGFGDNTRAALMTRG
                     LAEITRLGLAMGAQLPTFLGLSGVGDLVLTCSSRESRNHFVGEQVGKGRTLKEVLQGM
                     VTVAEGVKTAPAAIGLAEKWGVPMPIASEVNAVLFEEKKPQDAVTALMMRDPKPEHWS
                     "
     gene            complement(3982..>4207)
                     /locus_tag="P5179_11700"
     CDS             complement(3982..>4207)
                     /locus_tag="P5179_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-phosphate glycerol 3-phosphate
                     acyltransferase"
                     /protein_id="HRS95925.1"
                     /translation="GSITAAFVLAAGLVIQRLFFPESLPIPVVVIGCVVAAVVIVKHR
                     TNIQRLINGTENRFGKPASATLAEKEGHRS"
BASE COUNT          743 a         1352 c         1206 g          906 t
ORIGIN      
        1 gtgggtgtag atctgggtgg tggagatgtc ggcgtgtcca agcatttcct ggactgcccg
       61 gaggtctgct ccgccctcaa gcaggtgcgt ggcgaacgaa tgtcgcaacg tgtgcgggct
      121 gacggttttc gtgataccgg tcttacggca gtaattcttg agcatctgcc agaaggccat
      181 cctcgtcaaa ggcctgcccc tccagttcaa aaagagtacg ttgcccgact ctggttttgc
      241 aactgcgctg cgcgccgtag caacataggc gctgatgcac tctaccgcct ttcgtccgat
      301 ggggactaga cgttccttgt tcccttttcc ggttacgcgg atgaagcctt cgttgaaaaa
      361 caggttgcgt atgtcgatgg aaatgagctc acttacgcgg agaccggtcg cgtacgcgca
      421 ttccagcata gcacggtcgc gaagcccaag tggagtaacc gggtcgggtg cgttcagaag
      481 acgctcgatc tcgtttcgac tgagaacctc gggaagattt cgctcgagtt ttggcgtatc
      541 aagggtgccg gtcgggttgg attccgccac accttcgttc tgcagaaacc tgtgaaaaac
      601 gcgcacggcg gacatcgttc gagcgatact gctcggcgca aggttctggc cccgcaacca
      661 cgaaaaaaaa gacgacacgt cctccctggt tgctgttcgg ggagtcattc ccccgcgttt
      721 cgtttccaga aaacgcacgt aacggtccag gtcccgtcgg taggcagcct gggtattctg
      781 cgaaaggccc cgctcagccg tgatgtactg cagaaacgaa ctcaggaggc ggagccccgg
      841 caactgagcg ggtgacgatt ccgctccgcg agatgcgtcc cgggcaatcc gtggtgagga
      901 cttcacggat ttcctctcca tttaaccccc ctcaaacctc gctacgcatc agacgacttc
      961 tcatctccgg atgagcactc agcgagtgcc cgtccgtcgc tgacatcctt cgttgcctcg
     1021 gtgctttcgg gagacggatc aaccagcatt ctcaccatct cggcgatggc cgccagttcc
     1081 tcacgtaacg cgcgcaggtc ctcgaccaca gattttggaa gagtctccgg gactggcacc
     1141 gcctcggacg cgatgagctc gaaaacctcc cgcctttccg gacgcggcgg ctcgatctcc
     1201 tcgtgggtgg aaagctcggt ttgtgtgtcc gccggcatgg cgaggagggg tgagattgcc
     1261 tcttcttcct cggtgtcggt ctcgcccggt tctgtttccg aggccacatt cgcatcagaa
     1321 ggagcacccg tttccggcgc ggattcaggc tgctcctgcg ggaacgccat attggcaatg
     1381 gaatcccgcg tcccgtcagc accttcgttt tctggcgctt ccacgaagcc gctctccgcg
     1441 atctgcgctg ccgtttcggc agcctccgcc gcgatctcac tctccgcttc ccaggttatc
     1501 gcagttgcgg acgggagtgg ttcgggtccc ggaaccgacg cggaggcggg cgtttcggca
     1561 acgtcggtca caggtgcccc gtcccatggt tgcacagcgg gggatggttc gctttcttcg
     1621 gcagcaacct cgggaacgac cggctgattc aggtgctctt tctcccccga ttccgcgagc
     1681 tccggctccg gttcgaactc ggaatccggc aaagaagctt cgcggggcgt cgccgcttcc
     1741 ggctctccct gcaaagaggc ctcgccctct tcttccaccg gcgtccaaac cggcgccgcc
     1801 atgccgattt cttccgtcgc ctctccagcc gccggcccgc ccgctgtctc ttcggccatt
     1861 ccttcgctgt ggacgaatga ctcgatctgt tccgccgcgg cgaaaacctg ctcgcgatcc
     1921 tcttcttccg ggtggtaacc cgtctcggta gctggtgaca catcctcagc cgtggcgatc
     1981 tggtcccccg gcaacaccgg ttgctccatc agattggctt caaccggaac gcttggtgtc
     2041 gcatcttgga cctccacggg cgccaattcc tcgcgttcaa ttggcggcgc ggacgcggcg
     2101 agatccatgt ctccgtctga agtctcaacg acaggctctg tggaggtagg agcctccacg
     2161 ggttcccgtt caggttcggc cggagtctcc gtagcaaagg gagggagtcc ggtgtcctgc
     2221 catgtctctt cagcttccga cacaccttcg gcgggaaaag agcgctcttc ggcggacatc
     2281 tcgcccgacg ccggttcggc cgtctcttcc gcttcggcgg cgccgacaac ggtcgatggt
     2341 tcttcggtga gcggaagcgt tggtgaagaa gcgcccgcga gttcgcgttc ttccgtttcc
     2401 gcaggtgggt atgctgcggc accaaccgcg ggagtctcga caacttcacc accgccggag
     2461 cccggttctt cagccggagc ctccgtcact tcttgttcgg tttcccgcgg caccgcggct
     2521 cctgcaagcc gaacgcccgc ctgagccggt tcttcgttct tgtcctcatg aggagttgca
     2581 gcgacctccg gtttttcaac tgtgcgcgct gttgtggacg gttcattctc gtcgggtgtc
     2641 ccctccagcg cttccggggg caccgcgttg atcatggccc gaatttcgtc gggcgcggcc
     2701 accagaagct ctttcacgtc ggaaaccgac cacttttcct tctcgatgag ctgttgcaga
     2761 acgagaacga tgcggaaatc tcgctcccgg tacacccgta cgccgctcgc atcgcgccgc
     2821 ggacgcaata tggccagttc ctgctcccag gctcgcagcg tttgctggga gattccggtt
     2881 atttctgcga cttcaccaat acgccagtac aggcgcgtga atctgcgttt gagcattttc
     2941 acctcggatt gcaggacgaa caaccgtccc gcggatggat tcagctccag tgttcgggtt
     3001 tcgggtcacg catcatcagc gcagtgaccg catcctgtgg tttcttttct tcgaaaagaa
     3061 ccgcgtttac ctcgctcgct atcggcatcg gcacgcccca tttctccgcc agaccgatcg
     3121 cagcgggggc ggtcttcact ccttctgcga cggtaaccat gccttggagc acctctttca
     3181 gcgtccggcc ctttccgacc tgttccccga cgaaatggtt tcggctctcc cggctggaac
     3241 acgtaagcac gaggtctcct acgccggaaa ggccgaggaa ggtgggtagc tgcgcgccca
     3301 tagccaatcc gagccgggtg atttcggcga gccctcgtgt catcaatgcg gcgcgggtgt
     3361 tgtccccaaa cccgagcccg tccgacatac cggcggcgat tgcgatcaca tttttcagtg
     3421 ctccgccgag ttccacgccg agggaatcgg gtgagcaata cacacggaat gtgctgctca
     3481 tgaatgcccg ctgagccgct accgcagctt tctccgaccg tgccgccgca acaaccgtcg
     3541 tcgggtgacc caggccgaca aatcgcgcaa aacttggacc ggaaagcgcc acgatttcgt
     3601 ccggaggtac ctcgggaagt tcctccgcga gaagttcgga catacgcttg aaagaaccct
     3661 gctcgatgcc cttggctgcc gtgacgacca gtttgccctg ccagttggca gatttcactg
     3721 aacgcgccat atcgcgcatc aattcacacg gaccgggaaa caccaccatg tcggcacttt
     3781 ccagcacatc gtccggtgcc gaaagaattg ccactgcgtc aggaatcggg acgccgggga
     3841 gcgccttccg gttttcgcgg tacgtcagaa gttccgtgcg caaagcctca tgcggcgcga
     3901 tcaggctgac tctgtgccca tttctggcca gaagccccga caggacgctt ccccaactgc
     3961 cggcccgcat cacgacaaca ttcacgaacg gtgcccctct ttttccgcaa gagttgctga
     4021 cgcgggtttg ccgaaacgat tctcggtgcc gttgataagg cgctggatgt tcgtgcggtg
     4081 tttgacgatt accaccgcag cgacaacaca cccgatcaca actaccggta tgggcaacga
     4141 ttcagggaaa aacagacgct gaataaccag acccgctgcc agcacgaacg cagcggtgat
     4201 cgaccct
//
LOCUS       DAOTNG010000277         4206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17097_length_4206_cov_5.221152, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000277 DAOTNG010000000
VERSION     DAOTNG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17097_length_4206_cov_5.221152"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="P5179_11705"
     CDS             complement(<1..1179)
                     /locus_tag="P5179_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS95926.1"
                     /translation="MKRMFYAAVMIACSGVLGSPVRVAAQVTVVKPELTAEQALALNA
                     VRDSARTLFQQRKYRDAVEAYKRSLLIDSTDAATYFNLGLGYQVLGNDDEALAMLNRS
                     VALDSLFAPAHLEVGKVYLKKQELEQAEEAYLATKRIFADSLQYSMAADQGLNNVAIA
                     YSNRAVVGMRERNFGSAEADAEKAISLSPDISRGYVVAATVYENQQKYDEAEQRYRAA
                     VDRAENDQVKANALEGIARSILGRVRDSRNNTAGVRSARVSAAEILRQAVQIDTTNFT
                     ALVNLGNTLFDLEQYAAAESALVRAESLRPRDFRPPLKLAETYMKLNKCGEAEQAATR
                     ALAVQPGNASAHATRAEALECLGRAREALDAYVQASRDPRWKQYADRKAKTLREQLGL
                     P"
     gene            complement(1388..2056)
                     /locus_tag="P5179_11710"
     CDS             complement(1388..2056)
                     /locus_tag="P5179_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS95927.1"
                     /translation="MKWFHLTLLVLFACSPIVVPEKPTIVTQAVPVYPESARQEGIEG
                     DVLVEMVVGADGMVQDVQILSGPSVFHESAREAAARMVFSPARQDHQSVRVKVLQRFS
                     YRLSSQALASRDSTAVPSLNEEIPLLEYAEVEVKPFPVGPPVPEFPEIARGVGFGGEV
                     VLDIIIGADGNVESAVVREGPPEFRGAAREAAKKMVFTPGMHGNRPVRVKAVQRIRFP
                     FSPQ"
     gene            complement(2236..2901)
                     /locus_tag="P5179_11715"
     CDS             complement(2236..2901)
                     /locus_tag="P5179_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS95928.1"
                     /translation="MVGTSVKPRRDIVKTIYRPVLLVSTLVSAFLCVGFYNLNMRMEK
                     ERISSQKQGQAIEVVDIPATAQQQEQAAAPARPAVPVEARDESQVQDLTIMDTELYQQ
                     ASVAVPEKLQEASNISQQVEQEAPPMEFWMVEEKPTIVTQAIPVYPEIARQAGMEGDV
                     IVEMVIGTDGKVQSVQILRGPPVFHDAAREAAMKMTFTPAKQNDRPVRVKVSQRISFR
                     LRG"
     gene            complement(2917..3345)
                     /locus_tag="P5179_11720"
     CDS             complement(2917..3345)
                     /locus_tag="P5179_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS95929.1"
                     /translation="MALVGGKFKGPSQPEVVIPTASMADISFLLLIFFIVSTVFVRFR
                     PEFQPQLPKAESVDALKNRRNISYLWISPDGIIQIDSSLVNLNSVAGVMLKKIQKNPQ
                     LIVLIKSDEKTRYGMVSNVIEELRKANAIRIAFAAKSELE"
     gene            complement(3387..3851)
                     /locus_tag="P5179_11725"
     CDS             complement(3387..3851)
                     /locus_tag="P5179_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS95930.1"
                     /translation="MLMGKGNLAPSVEIPTASMADISFLLLVFFIVSTSMNQDRGIGM
                     TLPPPGNPMPIPKENIASVWINNRGEVALKYLGQDYITVTLPEMRERIERRIIENPNL
                     VVSLLAQQDADYKTFIDVLDELRMVGSRHDWSKISIAQPFGSAGVGGGPGGQ"
     gene            complement(3877..>4206)
                     /locus_tag="P5179_11730"
     CDS             complement(3877..>4206)
                     /locus_tag="P5179_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS95931.1"
                     /translation="ASFLQRGMIVIASVTVIAPFFGFLGTVWGMVIAFKAIAEAGDVN
                     PTIVASGISQALITTVGGLIIAIISQSLYNFLLSRINRIVIDMEETSTKLVDLLIEEG
                     MDKPASS"
BASE COUNT          905 a         1335 c         1091 g          875 t
ORIGIN      
        1 aggcagaccg agctgctcgc gcagcgtttt cgctttccta tcagcatact gcttccaccg
       61 cggatcgcgg cttgcctgaa cgtacgcgtc gagcgcttca cgagcgcgcc cgaggcactc
      121 caacgcttcc gcccgggttg catgtgcgga ggcgttcccc ggctgaaccg ccagtgcacg
      181 cgtagccgcc tgttccgcct ccccgcattt gttcagcttc atgtacgtct cggcaagctt
      241 gagcggcggc cgaaagtcgc gcggacgcag actctctgcc cgcacaagag cggattccgc
      301 ggccgcgtac tgctccagat caaacaacgt gttgccaagg ttcaccagcg cagtgaagtt
      361 cgtcgtatca atctgtaccg cctgacgcag gatttcggcg gcagagacgc gcgccgaacg
      421 aaccccggca gtgttgttgc gggaatctct cacgcgcccg aggatcgatc gcgcgatccc
      481 ttcgagagcg ttcgccttca cctgatcgtt ctccgcgcga tccactgcgg cacgatagcg
      541 ctgctcagct tcgtcatatt tctgctggtt ctcgtacacg gttgccgcaa cgacgtaccc
      601 gcgcgatatg tccggagaaa gcgaaatcgc cttctctgcg tccgcttccg ccgatccgaa
      661 gttcctctcg cgcatcccca ccaccgcacg attgctgtac gcgatagcaa cgttgttcag
      721 cccctggtca gcagccatgg aatactgaag ggaatcggca aaaatccttt tggtcgcgag
      781 atatgcctct tccgcctgtt ccagttcctg ctttttgaga tacaccttgc cgacctcaag
      841 gtgcgcggga gcgaaaagcg aatccaacgc gacggagcga ttcaacatcg caagagcttc
      901 gtcgtcattc cccagcacct gatacccgag accgaggttg aaatacgtcg cggcgtccgt
      961 cgagtcgatc agaagggagc gcttgtaagc ctccacggca tcccggtact tcctctgctg
     1021 aaacaacgtt cgggccgaat cgcgaaccgc gttcagcgcc agtgcttgct ccgccgtcag
     1081 ttccggcttt acgacggtaa cttgagcagc tactcgtaca ggcgaaccaa gaacgccgct
     1141 gcaagcgatc atcaccgcag cgtagaacat ccgtttcata acaacactcc ccgtttgatc
     1201 atttcctcaa cgccgtgtga caaccggcgg gaccggacgc aaccgttaaa aataacgtgg
     1261 aaacacgcgc cgcatgcaaa ccgagctttt cagtctgcct gcagaatgat tgtgcaaatg
     1321 gcgtaccgca cgcggaatcg aacgacaaag gcttcgcccg accggcattc cggcttccgg
     1381 cgagccgtta ctgaggagaa aaagggaacc ggatccgttg caccgccttc acccgaacag
     1441 gacggttacc gtgcattccc ggtgtgaaca ccatcttctt tgcggcctca cgcgcagcac
     1501 cccggaactc gggcggcccc tcacggacca ccgcactctc cacgttcccg tccgcgccga
     1561 tgatgatatc aagaacgacc tctcctccga aaccaacgcc ccgtgcaatc tcagggaact
     1621 ccggaaccgg tggaccaacc ggaaaaggct tcacttcaac ctcggcatac tcaagaagcg
     1681 gaatctcttc gttcagagac ggcaccgcag tggaatcccg agaagccagc gcctgcgaac
     1741 tcaagcgata actgaaacgc tgcagaacct tcacccgaac gctctggtga tcctgcctcg
     1801 caggcgagaa caccattctg gccgccgcct cccgtgcaga ctcgtggaac accgatggtc
     1861 cactcaaaat ctgtacatcc tgcaccatcc cgtcggcgcc cacgaccatc tccaccagta
     1921 catcgccctc aattccctcc tggcgggcgc tctccggata taccggtacc gcctgtgtca
     1981 caatcgtcgg cttttccggt acgacgatcg gagaacacgc gaaaagcacg agcagtgtca
     2041 ggtgaaacca cttcatccga ggccgcctct ggcaaaccgc gttcgcgcgc cggagccttc
     2101 ccgcgaggat acccatgtcc cgcgcgagtg catcgcgaaa acaagaaacc ctcacaccag
     2161 tgcctacccc gaaatgtacg aggtagaccg gtgtgagggt acaaccagcg atcaaaaaac
     2221 cgggattctg cgttctcagc cgcgcaaccg gaaactgatg cgttgcgata ccttcacgcg
     2281 tacagggcgg tcattctgct tcgcgggtgt gaaggtcatt ttcattgctg cttcccgcgc
     2341 cgcgtcgtgg aaaacgggtg gcccccggag aatctggacg ctctggactt tgccatccgt
     2401 accgataacc atctccacga tgacatcgcc ttccatccct gcctgacgcg cgatttccgg
     2461 atagacaggg atcgcctgcg tgacaatcgt aggcttttcc tccaccatcc agaactccat
     2521 gggcggcgct tcctgctcta cctgctggct gatgttggag gcttcctgca gcttttccgg
     2581 cactgcaacc gacgcctgct ggtaaagctc ggtgtccatg atcgtcaagt cctggacctg
     2641 actctcgtcc ctggcttcca caggcacggc cgggcgagcg ggagcagcag cctgttcctg
     2701 ttgctgagcc gtcgccggaa tgtccaccac ttcaatcgcc tgcccttgct tctgcgaaga
     2761 tatccgctcc ttttccatgc gcatgtttag gttgtagaac cccacgcaca gaaacgcaga
     2821 aacgagtgtc gaaacgagca gaaccggccg gtagatagtt ttgacgatgt cgcgacgggg
     2881 tttaacagag gtgccaacca tgccgtatcc cctccgtcat tcgagttctg atttcgcggc
     2941 aaacgcaatt ctgatcgcat ttgccttgcg aagctcttct atcacgttcg acaccatgcc
     3001 gtaccgtgtc ttctcgtcgc tcttgatcag cactatcagt tgcggattct tctggatctt
     3061 cttcaacatg acaccggcga ccgagttcag attcacaaga gagctgtcaa tctgaatgat
     3121 cccgtccggc gaaatccaca aatacgatat gtttcgccgg tttttcagtg cgtcgaccga
     3181 ctccgctttc ggcaactgag gctgaaactc cgggcggaac ctgacgaaca ccgttgatac
     3241 gatgaagaag atcaacagca aaaacgatat gtccgccatg gatgccgtcg gaataaccac
     3301 ctccggctgg ctgggaccct tgaatttgcc acctacaagc gccatgtata cgactcctca
     3361 cgtcgtggct ctgttccacc cgaaccttac tggccgcccg ggcctccgcc gacaccggct
     3421 gatccgaagg gttgtgcaat tgagatcttc gaccagtcgt gccgacttcc taccatgcgg
     3481 agttcatcga gaacgtcgat gaacgttttg tagtccgcgt cctgctgagc gagcaacgat
     3541 acgacaaggt tcgggttctc tatgatccgt cgttcaatcc gctcgcgcat ctccggcaac
     3601 gtgacggtaa tgtaatcctg cccgaggtat ttcagggcca cctcgccgcg gttgttgatc
     3661 cacacgctcg cgatgttttc cttgggaatc ggcatggggt tgcccggcgg gggcaacgtc
     3721 attcctattc cccggtcctg gttcatcgac gtgctgacga tgaaaaagac cagaagaagg
     3781 aatgatatgt ccgccatcga cgccgtgggg atttccacgg aaggagccag attgccctta
     3841 cccattaaca tggttcatat ccttcgcggt cgactgtcag gaactggccg gtttgtccat
     3901 gccttcctcg atgagaagat cgaccagctt ggtcgacgtc tcctccatat caattacgat
     3961 acggttgata cgggaaagga ggaagttgta cagagactgc gaaatgatgg cgataatcaa
     4021 cccaccgacc gtcgtgataa gtgcctgcga aataccactg gcgacgattg tcgggttcac
     4081 atcgcccgct tcagcgatgg ctttgaacgc gatcaccata ccccacacgg tgccaaggaa
     4141 cccgaagaac ggagcaatga cggtaacaga tgcgataaca atcattccgc gctgcaggaa
     4201 actcgc
//
LOCUS       DAOTNG010000278         4204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17105_length_4204_cov_3.735599, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000278 DAOTNG010000000
VERSION     DAOTNG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17105_length_4204_cov_3.735599"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>979
                     /locus_tag="P5179_11735"
     CDS             <1..>979
                     /locus_tag="P5179_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95932.1"
                     /translation="VFLLFLWQKHGTAALTGIRDTFAARDLSTATVITKLQNATGVAM
                     RDLLSEFWVWSYFSGDRYRPQFFAEGAHYKPAPLDSVRYPAAAKKGTLRSISSVRDTT
                     CTFSTQFLGAQLLRIVPDGSAGGVVVSLSGVSPNVNNWSLRLAVANSDSVTFVAVTID
                     KQTGVREAAIPPSIWRSAYDVILVAANDAVAGSAVAFSCRIRYVDPDAIGALGVVQIP
                     GGPTAFSVRNDARNVVVLRLRVTPSVTEQIGVSSLTVQAFGTADDSALVAPYGIKLHH
                     DINDDGLIDADVDTILARASGFPNDNGSTTLLLGGKVVTAAESWILSYD"
     assembly_gap    980..989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <990..1924
                     /locus_tag="P5179_11740"
     CDS             <990..1924
                     /locus_tag="P5179_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS95933.1"
                     /translation="PHGATFAVTIASDGILAVGLNSGFPMSPTGVFPIVGPTGTISQL
                     ATLRLDAGSSNPQDSREHRHAQNLVMLQFRLAASNVETVLVTRVTVAGDGTGDEAVDV
                     ACVHLRRDNNGDGVYEPSVDDTLRYVGSRSFVQDDGTVSFDFAVPETIAAGTSQYWLV
                     VYHLALSVGPLPGSTFRAMLRSADYVAATGKASRAPVTVEAVFPIQGGWKTITQAGVD
                     RFELGQNLPNPFNPTTVIPFTLDRSSAVDLTVYDVQGRVVRRLISGAHLEAGDHRVTW
                     DGQTENGSSAGAGVYFVRLSAGNETRVRRMTLIR"
     gene            complement(1999..3237)
                     /locus_tag="P5179_11745"
     CDS             complement(1999..3237)
                     /locus_tag="P5179_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS95934.1"
                     /translation="MAERAKYGAAAMAGITGKLPVPFPREMGPNCLKYLEEYVASGLT
                     ADITGRFEAAFARELGVKHCIATPGCTPALAMLASAFSFAPGDEIIVSPITDYGTLQG
                     LIRENYIPVFADTDPGCINISARGIEPKITDRTRAILVVHKTGLMCDMEGINTLAARH
                     GLIVYEDACQAVFSEWKGRFAGTFGLAAGFSFDSEKTMGSDVGGCVVTNDDELAERLR
                     FVGLSRGAAMKPHFGRLHVVPGYAYRMPNCTAAICLGQLEIIREQVVRIDRAARLLNK
                     LVARIPGILPLRIPDQQNVWSCWMYGIRIDPTQFRCTPEEFADQLSQAGIPGAGMGKY
                     YLMPAALTFLDENARNKVYPYSMPPASREYRYSEENCPHAKAFLDNFIRWSTLCAKYE
                     PEHCELAAAIIAEVAERNRI"
     gene            complement(3248..4054)
                     /locus_tag="P5179_11750"
     CDS             complement(3248..4054)
                     /locus_tag="P5179_11750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS95935.1"
                     /translation="MNPERLRRIDSLTPGVNAREDGMWKNGARWVYSITYDEGCAALL
                     DHALPVHRRYGVPGHVAVLASQIGVPRNVPGSSYHGMMILSADEIHSLRAEGWGVSCH
                     SMTHASITPENAHYEVVNSRAVLEKVLGIPVPLFCVPGSNDCYPASLAVAREAGYSGI
                     MTIFDDINTRQTDLMRLCRVPLHTEYPPPFFSAFDPFKRIQQAIEAGGWIIDYCHCPM
                     PGKPIHPWKDCTVEQLEERFQAIRRFGGDEVWLAEPGEVVEYLLSAKHTP"
BASE COUNT          821 a         1265 c         1210 g          898 t
ORIGIN      
        1 tggtattcct gctcttcctc tggcagaaac atggcaccgc cgcgttgaca ggcattcggg
       61 ataccttcgc cgcgcgtgac ctcagcacgg caaccgtcat caccaaactc cagaacgcca
      121 ccggcgttgc catgcgggat ctcctcagcg agttctgggt gtggagttat ttctcaggtg
      181 accgctaccg ccctcagttc ttcgccgagg gcgcgcacta caaacctgcg cccctggact
      241 ccgtgaggta tcctgccgcg gcaaagaagg gtaccctccg ttccatttcc agcgtcagag
      301 atacgacatg cacgttttcc acccagtttc ttggagccca gttgctccgg atagttcccg
      361 acggttcggc aggcggcgtg gttgtcagcc tatctggtgt gtcaccgaac gtgaacaact
      421 ggtccctgcg actggccgta gccaattcgg attcagttac gttcgtcgcc gtaaccattg
      481 acaaacagac aggcgttcga gaggcagcaa taccaccaag catctggagg agcgcgtacg
      541 acgtcatttt ggttgcggcc aatgacgcag tggccggttc cgcagtcgcc ttctcatgcc
      601 gaatccgata cgttgacccg gacgcaattg gggcgctcgg cgtggtgcaa attccgggtg
      661 ggccgacggc attttcggtt aggaacgacg ccaggaatgt cgtagtactg cgactgcggg
      721 tgacaccatc ggtaacagag cagatcggag taagcagttt gacagttcaa gcatttggta
      781 cggcagatga ttccgcgttg gttgccccat acggcatcaa actccaccat gacatcaacg
      841 acgatggact gatcgacgcc gacgtcgaca cgatcttggc tcgggcttca ggtttcccga
      901 acgacaatgg ttccaccact ctgttgcttg gcggcaaggt agtgacagcc gcagagagtt
      961 ggattctctc gtatgacttn nnnnnnnnna gccccatggc gccactttcg ccgtgacgat
     1021 tgcgtcggac ggcatattgg cagtcggctt gaacagcgga tttcccatgt caccgaccgg
     1081 cgttttcccg atagtcggac caacaggaac gatcagtcag ttggcgacac tcagactgga
     1141 cgccggttcg agcaacccac aggacagccg ggaacaccgt cacgctcaga atcttgtgat
     1201 gttgcagttc aggttggcgg cttcaaatgt cgaaaccgtg cttgtcactc gggtaaccgt
     1261 tgcgggtgat ggaaccggcg acgaagctgt tgacgtggca tgtgtgcacc tgcgccgcga
     1321 caacaacggg gatggggttt acgaaccctc cgttgacgac acacttcgtt atgtcggctc
     1381 gcgtagtttc gtgcaagacg atggaaccgt ttccttcgat tttgccgtcc cggaaacaat
     1441 cgctgcggga acgtcgcagt attggctagt tgtgtaccac ctcgccctat ccgtggggcc
     1501 gttgcctggg agcactttcc gcgcaatgtt gaggtcagca gactatgtgg cggctactgg
     1561 taaggcttcg agggcgccag tgacagtgga ggcagttttc ccgattcaag gtggctggaa
     1621 aacaataaca caagcggggg tggaccgctt tgaactcggt cagaaccttc caaacccctt
     1681 caatcccacg acggtgattc cgttcacgct tgaccgttcg tctgcggtgg acctcaccgt
     1741 gtacgacgtt caggggcggg tggtgcgccg gctcatttcc ggggcgcatt tggaagccgg
     1801 cgatcacagg gtaacgtggg atggtcagac agaaaacggt tcgtccgcag gtgccggcgt
     1861 gtacttcgtc cggttgagcg cggggaacga gacgcgtgtg cggcggatga cgttgattcg
     1921 gtgaacgtcc cgcgactacc cctgcgtggc ctctcatgtg tgcaggaacg ccttcactgc
     1981 gcccgccctg ccctcgtgct agatccgatt cctctccgcg acttccgcga tgatcgccgc
     2041 cgccagctcg cagtgctcag gctcgtattt cgcacacaaa gttgaccacc ggatgaaatt
     2101 gtcgaggaat gcctttgcgt gagggcagtt ctcctcactg tagcgatact cccgactcgc
     2161 cgggggcatc gagtagggat agaccttgtt ccgtgcattc tcatccagaa acgtcagcgc
     2221 tgccggcatc aggtaatact tccccatccc cgcgccggga attccggcct gggaaagctg
     2281 gtcggcaaat tcctccgggg tgcaacggaa ctgagtcggg tcaatgcgaa ttccgtacat
     2341 ccagcacgac cagacattct gctggtcagg aatcctcagg gggagaatgc cggggatccg
     2401 cgccacgagc ttgttgagaa gccgcgccgc gcggtcaatt cgaaccacct gctcccggat
     2461 gatctccagt tgtccgaggc agatcgccgc ggtgcagttg ggcatgcggt aggcataccc
     2521 cggcaccacg tggagcctcc cgaaatgggg cttcatggcc gcgccccggc tcaggccgac
     2581 aaaccgcagg cgttcagcca gttcgtcatc gttggtgacc acgcatccgc caacatccga
     2641 ccccatcgtt ttttcggagt caaaggaaaa acccgccgca aggccaaagg tgcccgcgaa
     2701 gcgccccttc cattcgctga atactgcctg acacgcgtct tcgtacacga tgagcccgtg
     2761 tcgcgccgcg agggtgttga tgccttccat gtcgcacatg agacccgttt tgtgaacgac
     2821 gaggatggcg cgcgttcgat ccgtgatttt tggttcgata ccccgtgcgc tgatgttgat
     2881 acaacccgga tcagtatcgg cgaacacggg gatgtaattc tcgcggatca agccttggag
     2941 cgtcccgtaa tcagtaatcg ggctcacaat gatctcatct cccggcgcga aggagaacgc
     3001 ggacgcaagc attgcaagtg cgggcgtgca acccggggtt gcgatgcagt gcttcactcc
     3061 cagctcacgg gcgaatgccg cttcgaaacg gccggtgata tccgcggtaa ggccgctcgc
     3121 aacgtattcc tcgagatatt tgaggcaatt cggccccatt tcgcggggga atggcaccgg
     3181 cagcttcccc gtgatgccgg ccattgctgc ggcgccgtat ttcgctcgtt cggccatcac
     3241 attctcctca tggtgtgtgt ttcgcgctca agagatactc caccacttcg cccggctcgg
     3301 ccagccagac ttcatcgcct ccaaaacgcc gtatcgcctg gaagcgctcc tccagttgtt
     3361 ctacggtgca gtctttccac gggtgaatcg gcttcccagg catggggcag tggcagtagt
     3421 caataatcca cccgccggcc tcgatggcct gttgaatgcg cttgaacgga tcgaacgcgg
     3481 aaaaaaacgg cggcgggtat tccgtgtgaa gtggaaccct gcaaagcctc atcaggtcgg
     3541 tttgccgcgt gttgatgtca tcgaaaatag tcatgatgcc cgaataaccc gcctcccgcg
     3601 caaccgcaag tgacgcgggg tagcagtcgt tgcttcccgg aacacagaac aacggcaccg
     3661 gaatccccag caccttttcc agcaccgcac ggctgttcac tacttcgtag tgagcgttct
     3721 cgggcgtgat cgaggcgtgg gtcatgctgt ggcatgaaac accccatcct tcagcacgca
     3781 gcgaatgtat ctcatctgca gagaggatca tcataccgtg gtaactgctt cccggcacat
     3841 tacgtggcac gccaatctgg ctcgccagaa cggccacatg ccccggcacg ccatatcttc
     3901 ggtggaccgg gagcgcatgg tcgagcagtg ccgcacatcc ttcgtcatac gtgatggagt
     3961 agacccaccg tgcgccgttt ttccacattc cgtcctcccg cgcgttcacg ccgggcgtca
     4021 atgagtcaat acgcctgagt cgttccgggt tcacttcctc ggctctacga ttttgccgta
     4081 cttctccggc ttcctcgtgg tgtggaacat ggttcggaac tccttgtact gctcccccca
     4141 gaacggcata cgccatttcg ggtcgaattc ggccacggcg acccccgcct gatgccctgt
     4201 ggac
//
LOCUS       DAOTNG010000279         4175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17245_length_4175_cov_2.729854, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000279 DAOTNG010000000
VERSION     DAOTNG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17245_length_4175_cov_2.729854"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="P5179_11755"
     CDS             <1..885
                     /locus_tag="P5179_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRS95936.1"
                     /translation="VPSEEFEVFSLEDCVSQAFEAMEKRARCCRKYAGASSGYDELDM
                     ITGGFRDGEFIVIAARPSVGKTAFALNLFRNMSAQGIPAAFFSLEMARDPVTTRLLSS
                     VSQVEEHRLNTGEVLARGDDEDAIALRKAGEILSALPGFIGDPGKNMTCTQIRAKARR
                     LVHSPDTKVRAVFIDYLHLIEPTERTDNRVQELERISKNLKALARELNVPVVVLSQLN
                     RETEQSGRKPKLHNLKGSGAIEQDADIVIMLDRDDRRGNSEPGFLNLTVEKNRNGRTG
                     ECSLRCRPDIQLVESLKD"
     gene            1774..>4175
                     /locus_tag="P5179_11760"
     CDS             1774..>4175
                     /locus_tag="P5179_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRS95937.1"
                     /translation="MPRIFDNIENDLLPALQDTMRVAQRGDFCVGYFNLRGWKHLAGY
                     VEQWQGGDGACCRLLLGMQPRPSEVLFRVFRAQTIDNKTAVRLKTEVATELRQQLARG
                     IPNDEEETALRRMVRQIRAGKLIVKVFLRHPLHAKLYMLHREDVNNPITAFLGSSNLT
                     LAGLVNQGELNVDVLDHDACAKLQGWFEDRWNDRFCLDISDELVRVIGESWASETPLP
                     PYHIYIKMVYHLSQEARAGAIESVIPPDLAKTLFPFQQEAVKIAAHHLRRPERGGVLI
                     GDVVGLGKTLTATAVLKAFDDLNPLILCPANLVRMWEDYRERWELRGRVLSTGRMLKE
                     LPRLQRYRLLILDESQNFRNREGKRYRALREYIATNDCKVILLSATPYNKSYGDLGAQ
                     LGLFVPEERDLGIRPNRLIASLPGGEKDFVRKYQCSPRTLRAFEASSEPEDWRDLLRM
                     FMVRRTRRFIMESYATKDESAGRYYLTMENGERAYFPIREPKTVTYPVNEADQFARLY
                     RESVVNMIGGLNLPRYGLGEYVRKDLPADTTETEKEIIDNLGRAARRLLGYCRTNLFK
                     RLESSGHDFLQSVERHIVRNCVFLYALERGLPLPIGTQDSALLDTAFSDEDIDSLFAT
                     VVSPDEDEESTEGTVPPEEALSGDYAARAKRVYDIYRTHMKRQFKWIRADLFESDALI
                     KTLRHDTEEFVRLHRTLPEWDPAQDSKLQALVTLLKTTHPREKVLVFSQFADTVRYLV
                     EELRKAGVRQVEGVTGQSSNPTEAAHRFSPESNGKRDRIRPDDETRVLIATDVLSEGQ
                     NL"
BASE COUNT          947 a         1224 c         1217 g          787 t
ORIGIN      
        1 gtgccttcgg aggaattcga agtgttcagt ctcgaggatt gcgtgtcgca ggcgtttgaa
       61 gccatggaaa agcgagcacg ttgctgtcgc aaatacgcgg gtgcttcgtc gggatatgat
      121 gaactggaca tgatcactgg cggctttcgt gatggcgagt tcattgttat cgctgcgcgt
      181 ccttccgtag gaaaaacagc atttgccttg aacctgttcc gaaacatgag cgcacagggc
      241 atcccagcgg cgttcttttc tttggaaatg gcacgcgacc ccgtgaccac tcgtttgctg
      301 agtagcgttt cacaagtgga agagcaccga ctcaacaccg gtgaagttct tgcacgtggg
      361 gatgatgaag acgccatcgc gcttcgaaaa gcgggcgaaa tcctttccgc attgcctgga
      421 ttcatcggag atcccgggaa aaacatgacg tgtacgcaga tccgcgcaaa ggcgaggcgc
      481 ctggttcatt cgcccgatac caaagtgcgg gcggtgttca tcgactacct gcatctcatt
      541 gaaccaacgg aacgaacaga caatcgtgtc caggaactgg aaagaatatc caagaacctg
      601 aaggcacttg cccgcgagtt gaacgtgccc gtggtagtgc tctcgcaatt gaaccgtgaa
      661 accgaacaaa gcggccgaaa gcccaaactg cacaacctca agggctcagg cgcgatagag
      721 caggacgccg atattgtgat aatgcttgac cgagacgatc gcagagggaa tagcgagccg
      781 ggcttcctga acctaaccgt ggagaagaat cggaacggcc ggaccgggga gtgctcactt
      841 cggtgccgtc cggatattca gctcgtagag tccctcaagg actgaggtca acgatgccta
      901 atcggaacgc cgtgggtacg gcggcacact tatgcagttg gtcatttggc aaacgactcg
      961 gtttgtcaag tcccttcagt tggtggaccc cgcgggacgc cgacctcgga gcaacaggag
     1021 ttcactcggc gagccttccg tgctcaacca gtgctccatc acgcaatccg agcggcagac
     1081 aggctgaaca gcgatcaaat cataccccac ctgtgcgcaa aggcgggccc attggggggc
     1141 atcgggatac gctcggttcc cggcgccctc gaccgagctt cagttcgtcg aggcagttga
     1201 catacgggta atccgagcat gcgctccgcc gatacctccc cgctcagatc cccacactaa
     1261 gttgacataa ccgttcttat catccgtgcc cagtttcgtc tccggcaccc ttccacggcc
     1321 ataaataggc gtacacggga aaacaacacc gcgtttcgca tcggcaacgc tttgcctgtg
     1381 taagtgtatt tttcacagaa agttagacac ggttgccttt ccgttgtctc ttgcgaaccc
     1441 gaatggggag gcaccgttgc tgcgaaacgc gggacaggtt ggtcgcagga ggagttcccg
     1501 tgtcgcgcct gttcgcgact gttctgtgtg agagctgtct ccaccgaccc cccgcccgcg
     1561 catgccaggc ttggatttcc cgaaatggaa cagcgttccc gttcccgcgg ctggttttcc
     1621 ctactaagga aagcaggaac ggaacgattg aaagctcgcc ttcaggggtt cccggacccc
     1681 ctgaggttct gctggaaaga ccgggttgcc catctgcaag aatttggtga ccttagaaca
     1741 gaatgtggtg gtacatacgg ctggggaggt ggtatgccgc gcatttttga caacatcgaa
     1801 aacgatcttc tgcccgccct gcaggacacg atgcgtgttg ctcaacgggg cgacttctgc
     1861 gtcgggtact tcaaccttcg cggttggaag catcttgccg gatacgttga gcagtggcag
     1921 ggtggcgacg gggcctgttg ccgactctta ctcggaatgc aacctcgacc gtcggaggta
     1981 ctgtttcggg ttttccgcgc gcaaaccatc gacaacaaaa ccgcagtccg cctgaaaacc
     2041 gaggtcgcaa cagaactgcg tcaacaactg gcacgtggca tcccgaacga cgaggaagag
     2101 accgcgttgc gccggatggt gaggcagatt cgggcaggaa aactcatcgt caaggtgttc
     2161 ctgcgccatc ctctccacgc caagctctac atgctgcacc gtgaggacgt gaacaatccc
     2221 atcaccgcgt tcctgggaag cagcaacctc actctggccg ggctggtaaa ccagggcgaa
     2281 ctcaacgttg atgtcctcga tcacgacgcg tgcgccaagc tccaaggatg gtttgaagac
     2341 cgatggaacg accgcttctg tcttgacatc tccgatgagc ttgtccgcgt gataggggag
     2401 agctgggcca gcgaaacgcc cctgccgccg taccacatct acatcaagat ggtataccac
     2461 ctttcgcagg aagcgcgcgc gggagcgatt gaatcagtca ttcctccgga cctcgcaaaa
     2521 acgcttttcc cgtttcagca ggaagccgtg aaaatcgctg cgcaccacct gagaaggccg
     2581 gaacgcggcg gcgtgttgat cggcgatgtg gtaggactgg gaaagacact caccgcaacg
     2641 gcagttttga aggcgtttga cgaccttaac ccgctcatct tgtgcccggc aaacctcgtt
     2701 cgtatgtggg aagattaccg ggaacgttgg gagcttcgag ggagagtcct gtccaccggc
     2761 agaatgctca aagagctgcc acgactccag cggtaccggc tcctcatact ggacgagagc
     2821 cagaacttcc gcaaccgcga aggcaagagg taccgcgcac tgcgggagta catcgccaca
     2881 aacgactgca aagtcatcct gctctcggcc accccgtata acaagagcta cggggatctc
     2941 ggcgcacaac tcggtctctt cgttccggaa gagcgcgacc tcggcatccg cccgaacagg
     3001 ctgatcgcgt cgctccccgg cggggagaag gacttcgtcc gcaagtacca gtgctcgccg
     3061 agaacgctgc gcgcgtttga agcgagcagc gagccggagg actggcgtga cctcctgcgc
     3121 atgttcatgg tgcgccgcac tcgcaggttc atcatggaga gctacgccac gaaggacgag
     3181 agcgccggaa gatactacct caccatggaa aacggggaac gggcgtactt cccgatcagg
     3241 gaaccgaaaa ccgtcacgta ccccgtcaac gaggcggacc agttcgcgcg tttgtaccgg
     3301 gaatccgtcg tcaacatgat tggtggcctg aacctgccgc gctacggcct cggggaatac
     3361 gtgcggaagg acttgcccgc agacaccacg gaaaccgaaa aagagatcat agacaacctg
     3421 ggtcgagcgg cccggcgcct gctggggtac tgccgcacca acctgttcaa gcgcctcgaa
     3481 agcagcgggc acgacttcct tcagtccgtg gaacgccaca tcgtgcggaa ctgcgtcttc
     3541 ttgtacgcac tcgaaagggg attgccgctg cccatcggca cccaggattc cgcgctgctc
     3601 gataccgcgt tcagcgacga ggacatagac tccctgtttg caacagtcgt ttcccccgat
     3661 gaagacgaag agtccactga gggaacggta ccgccggagg aggctctcag cggagactac
     3721 gccgcgcgtg cgaagcgagt gtacgatatc taccggacgc atatgaagag gcaattcaaa
     3781 tggatcagag ctgatctgtt cgaatcggat gcactgatca aaacgctgcg gcacgacacc
     3841 gaggagttcg tccgcctgca cagaaccctc cccgagtggg acccggctca ggactccaaa
     3901 ttgcaggctc ttgtaacgct gctcaagacc acacacccgc gggaaaaggt gctcgtcttc
     3961 tcccagttcg ccgataccgt gcgctatctc gttgaggagc tgcgaaaggc aggcgtgcgg
     4021 caggtcgagg gcgtcaccgg tcaatcgtca aaccccacgg aagcggcgca tcggttcagc
     4081 cccgagagca acgggaaacg agaccgcatc cggccggacg acgaaacccg cgttctcatc
     4141 gccacggatg tgctgagcga agggcagaac ctcca
//
LOCUS       DAOTNG010000280         4156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17342_length_4156_cov_6.947086, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000280 DAOTNG010000000
VERSION     DAOTNG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17342_length_4156_cov_6.947086"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5179_11765"
     CDS             <1..478
                     /locus_tag="P5179_11765"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HRS95938.1"
                     /translation="AKTPGPQAAAEKASIMTAGALLGARHFVCAGTLSLDEIFSAEQL
                     LLDCEIAAHVEAVVRGLDTSCDPDACVQDVIEGASRGFLGLDSTLQRYRESCWYPRFF
                     ERGLLAAWQDSGSRELNERLREEARRLPSRHAFALPEEVQRELDRIYRRAEEHFSI"
     gene            647..1702
                     /locus_tag="P5179_11770"
     CDS             647..1702
                     /locus_tag="P5179_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95939.1"
                     /translation="MSIGIGIIGTGGISNSHVRGYQANASLGKIVAVADIDGARAEEA
                     ASRWGVDYFTTDYREILARDDVDVVSVCTYNQAHRQPTVDALAAGKHVLCEKPMAATL
                     DDAKAMAKAARDSGRILMIAIHSRYSPQTIAAKKIIDSGALGRVYYGETISTRRRWIG
                     SGSFARQETAGGGAVVDIGVYSLDTALHLLGHPKPKRVSGTTCMAITKDPSAQVYGGH
                     SWDVDRTNVEEFGAAWIRFENGLCITFKTSWAIHGNSMGRSFFLGEKGGVALGPLEYY
                     GDQFGMMVNVIPQDLPPEEDRFVLETRDFLECVRDGRPSPIPPEQVVVTNVIMDALYR
                     SAAADEEVEVTPWQDVL"
     gene            1684..2124
                     /locus_tag="P5179_11775"
     CDS             1684..2124
                     /locus_tag="P5179_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95940.1"
                     /translation="MAGRTVKARAPDAGKGGGTRMHAVAVRTTVAEYDLLDPRAPASA
                     IQACFQKPEVSWDASALEPGEVEAIQRATVRGAATQTIDIIREDRAVWVTYSVTMDFD
                     FNPNALNYFGVSIEDIIVRGCRVSFPIDENIVDLVEEKTTTRVT"
     gene            2146..2916
                     /locus_tag="P5179_11780"
     CDS             2146..2916
                     /locus_tag="P5179_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRS95941.1"
                     /translation="MRAMILAAGLGTRLLPYTKSLPKPLLPVNGKPLIAYPLGLVKEA
                     GVKDVVVNVYHLADQIESALKACSTMGLNIRFARERNLLETGGGLSNAQGFLGNETFF
                     LLNSDVVCDIDLKPVLEAHIRSGAIATMVLVEREGQSQYAPVVWDSRSKHVKDLRGEL
                     GVRTKCSRSLTFAGIHVMEPAVFDYLLKRKESIIDAFYLPALREGRHIHGYLHSGYWA
                     DAGTVQDYVRVRRDLAGVRFSHLKPVPLSAPIGLDIES"
     gene            2929..3924
                     /locus_tag="P5179_11785"
     CDS             2929..3924
                     /locus_tag="P5179_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRS95942.1"
                     /translation="MSAASSPRKHELSPIHGGASGRRYFRLSLAAQASDAAATYVVMV
                     VPTVEVGTFDAFCSNGEYLRSQSIPAPAILARWRDQGIALLEDFGTRTLTQVIASEQE
                     NRCALLEGAIGLLVRLHTCRQNDAFPCPAFQLHFDEEKWRFEYRFHVREWLIARHWKI
                     KPTRAESKILDRAFAWLGARLSREPRVFTHRDYQSSNLLVRDDGSYGLIDFQDARQGL
                     RQYDLASFLYDSYVDLNETERSELSNMYASMARLTISEDEHRYLVTISAIQRKLHDAG
                     AFAYAAYHREKTEYLCYLAPAVTQSVTLMAQIPHLREAGRVLSDYAARATGGTSA"
     gene            3921..>4156
                     /locus_tag="P5179_11790"
     CDS             3921..>4156
                     /locus_tag="P5179_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95943.1"
                     /translation="MSDVWLFFDIGDTLVNEDKLRFQIFKSLQKAITVETGHEFPFQE
                     LLALRERRLLEGELGVHYGIAKELLSPAGYDAWV"
BASE COUNT          891 a         1153 c         1256 g          856 t
ORIGIN      
        1 ggcgaaaaca ccgggaccgc aagccgcggc ggaaaaagcc agcatcatga cggcgggggc
       61 gcttctcggc gcgcgccatt tcgtgtgcgc gggaacgctg agtctggacg agatattcag
      121 tgcggaacaa cttttgctcg actgtgagat tgcggcgcac gtggaagctg ttgtccgcgg
      181 actcgatacg tcgtgcgatc ccgacgcgtg tgtccaggat gttatcgaag gagcgtcgcg
      241 gggattcctt ggccttgatt ccacgctgca acggtaccga gagtcgtgct ggtatccccg
      301 tttcttcgag cgtggccttc tggctgcgtg gcaggattcc ggaagccggg agctcaacga
      361 gcgactcagg gaggaagccc ggcgtctccc atctcgccac gcgttcgcgt tgccggaaga
      421 ggtgcagaga gaactggaca gaatttaccg gcgagccgaa gagcatttct cgatatgaca
      481 tgggggcgtt ccaaccgcca aagcgcttct cgaaaccccc agcgccagga gaggttcgaa
      541 ctggctccag tggcgcggtt cgaccccgag gtcgtatctt cttcgtaccg atcactcgtt
      601 cccgatatcg gaaccatgcc acaacatgtg gagcaaggag ccgcaaatgt ctatcggcat
      661 tggaattatc ggcactggag gcatttcgaa ttctcacgtc cgcggctacc aggctaacgc
      721 ctcgctggga aagatcgtgg ctgttgcgga catcgacggg gcgcgtgcgg aggaggctgc
      781 aagccggtgg ggtgttgatt actttacgac cgattaccgc gaaattctgg cgcgggatga
      841 cgtggacgtg gtcagtgtgt gcacgtacaa ccaggcgcac cggcagccca cggtagatgc
      901 ccttgcggcg ggcaaacacg ttctttgtga aaaaccgatg gcggcaacgc tcgatgacgc
      961 caaggcgatg gcgaaagcag ccagggacag cgggcgtatc ctgatgatcg caatccattc
     1021 tcggtacagc ccgcagacga ttgccgcgaa gaaaataatc gattccggcg ctttgggacg
     1081 tgtgtattac ggggaaacga taagcacacg gcgccgctgg atcggcagcg gctcgttcgc
     1141 ccgacaagag accgccggcg gtggcgctgt ggttgacatc ggagtctatt ctcttgacac
     1201 ggcgcttcat ctgctcggac atcccaagcc gaaacgcgta agtggcacca cctgtatggc
     1261 cattaccaaa gacccgtccg cacaggtgta tggcgggcat agctgggatg ttgaccgaac
     1321 aaatgtcgag gaattcgggg ccgcatggat tcgttttgaa aacggcctct gcatcacctt
     1381 caaaacatcg tgggccatac acggaaacag catgggccgc tcgtttttcc tcggggaaaa
     1441 agggggcgta gctcttggac ctctggagta ctacggcgac cagttcggga tgatggtcaa
     1501 tgtgattccg caggacctgc ccccggagga agacagattc gtactggaga cgagggactt
     1561 cctggaatgt gtgcgcgatg gtcggccgtc gcccataccg ccggaacagg tcgtggtgac
     1621 gaacgttatc atggatgctc tctaccgttc cgcagcagcc gatgaggagg tggaggtaac
     1681 tccttggcag gacgtactgt aaaggcgcgt gcacctgacg ccggaaaagg cggcggaacc
     1741 cggatgcatg cggtggccgt tcgaacaact gtcgccgagt acgatttgct cgatccacga
     1801 gcacccgctt ccgcgataca ggcgtgcttt cagaaaccgg aggtctcctg ggacgcttcc
     1861 gcgcttgaac cgggcgaggt ggaggccatt caacgagcca cggtaagggg agcggcaaca
     1921 cagacaatcg atatcatccg cgaagaccgg gcggtatggg ttacctattc ggtaacaatg
     1981 gatttcgatt tcaaccccaa tgctctcaat tacttcggcg tttcgatcga ggacattatc
     2041 gttcgcggtt gtcgagtttc gtttccgatc gacgagaaca tcgtggacct ggtcgaggaa
     2101 aagacaacca cacgggtgac gtaaaaagga caaaaaggca gttccatgag agcaatgatt
     2161 cttgccgcgg ggctcggaac gcggctgtta ccgtacacca aatctcttcc aaagccgctt
     2221 ctgcctgtca acgggaagcc tctgattgca tatccgctcg ggctcgtgaa ggaagcggga
     2281 gtgaaggacg tagttgtcaa cgtgtaccat ctggcagacc agatagaaag cgcgctgaag
     2341 gcatgctcca ccatggggct gaacatacgc ttcgcccgcg aacggaacct tctcgagacg
     2401 ggcggcgggc tgagcaatgc gcagggtttt ctcggcaacg aaacattctt cctcctgaac
     2461 tccgacgtgg tgtgtgatat tgatctcaaa cctgtcctcg aagcacacat tcgcagcgga
     2521 gccatcgcga cgatggtgct ggtggaacgg gagggccaga gtcagtacgc cccggtcgtc
     2581 tgggattccc ggagcaaaca cgtgaaggat ttgcggggag agcttggtgt ccgaacaaaa
     2641 tgcagccgct cactcacctt tgcgggaatt catgttatgg aacctgccgt cttcgattac
     2701 ctcctgaaga ggaaggaatc cataattgac gccttctatc tgccggcttt gcgggagggc
     2761 cgccacatac acgggtatct tcactcagga tactgggcgg acgctggaac ggtccaggat
     2821 tacgttcgcg tgaggaggga tctcgccggc gttcgttttt cgcatctcaa gccggtgccc
     2881 ctttccgccc ccattggcct cgacatcgaa tcttgatacg gtccggaatt gagtgccgct
     2941 tcttcacccc ggaaacacga gctttccccc attcacggtg gagcgtcggg gagacggtat
     3001 ttccgcctgt cactggcggc gcaagcgagt gacgcggctg caacgtacgt cgtaatggtt
     3061 gttccgacag tcgaggttgg aaccttcgac gcgttctgtt ccaacggtga gtacctccgc
     3121 agccagagta tccctgcacc ggcgattctg gcccgatggc gcgatcaggg aatcgccctt
     3181 ctcgaggact ttggaaccag aacgctcacg caggttatcg cgtcggaaca ggagaaccgt
     3241 tgcgcccttc ttgagggcgc aattggctta ctcgtgcggc ttcacacgtg ccggcagaat
     3301 gacgcgtttc cctgtcctgc ttttcagctt catttcgacg aggaaaagtg gcgctttgaa
     3361 taccgtttcc acgtccgcga atggttgatc gcgcggcact ggaaaataaa gccgacccgc
     3421 gctgaatcga aaatccttga tcgagctttc gcatggctgg gggcacgcct gagcagggag
     3481 ccccgggtgt tcactcaccg cgactaccag tccagcaatc ttcttgtccg cgatgacggt
     3541 tcctacgggc tcatcgattt tcaggacgcg aggcagggac tacggcagta cgacctggca
     3601 tcatttcttt acgactcgta cgttgacctc aacgaaacgg agcgaagtga attgtcgaac
     3661 atgtacgcga gcatggcccg cctcacgatt tcggaagacg agcaccgcta tctcgtgacg
     3721 atctcggcca ttcagcgcaa actgcatgat gcaggcgcgt tcgcatatgc tgcctatcac
     3781 agggaaaaga ccgaatacct gtgctacctc gcacccgcgg taacccagtc agtaacgctc
     3841 atggctcaga ttccacacct tcgcgaagca ggccgcgtac tctctgatta cgccgcgcgc
     3901 gccaccggag gaacgtcggc ttgagcgacg tatggctgtt ttttgacatc ggggacaccc
     3961 ttgtaaacga agacaagctc aggttccaga ttttcaaatc tctgcaaaaa gccatcactg
     4021 tggagacggg gcacgagttt cccttccagg agctgttggc tttgcgggaa aggcggcttc
     4081 tcgaaggcga gctcggtgtt cattacggca tcgcgaagga attgctttcg cccgcggggt
     4141 acgacgcctg ggtcga
//
LOCUS       DAOTNG010000281         4098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17611_length_4098_cov_3.845412, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000281 DAOTNG010000000
VERSION     DAOTNG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17611_length_4098_cov_3.845412"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="P5179_11795"
     CDS             complement(<1..1574)
                     /locus_tag="P5179_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRS95944.1"
                     /translation="MKCRVVSMNEWLYPDMAAGVSRPKPIVLHAARGTRTGFQVLFKP
                     TPANGELNVSLASQGGDFPSPEVFQLVDVMVEKNTGPIGFIAKEGESAEGYTTRLAPF
                     RVYDAMKPFLPGTKGRKGSEALFFEWRIPEDADPATYTDTIRVTIGKESLTLPVKLTV
                     HAATVTDPGTFSLTNWFNPGAMASRHGITPWTEAHWRMIRRYGELMRRGRQTHFWIPM
                     GYIGIKEGPEGLFSFDFAKAERLIRTYLKLGFTGIEGGHVAGRTEFHAPNFVLSTNHK
                     VNAVSREGYNFLAQYLPAWRDFLCRNGWLDIAVQHVGDEPTDKSMADYRVISGIVRKF
                     MPGTPIIDAVELSELAGAVDIWVPKDDYYEKHRDEFEFFRHHGDTIWCYTCCFPGGYY
                     MNRLLDMPLLRTRYIQWGCFKYDLPGFLHWGLNHYRHDQNPFECNCPDHGGGNNLPPG
                     DTHNVYPGDDGPWGSVRLEAQAAGIEDYELLRQLKEVDPESALKILSSCFRGFRDADE
                     NPQRFAAAHLNLLKAVS"
     gene            complement(1616..2542)
                     /locus_tag="P5179_11800"
     CDS             complement(1616..2542)
                     /locus_tag="P5179_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS95945.1"
                     /translation="MKLPENTGTPHAQTKNREASTKGTSQGWPASFEDDVPLVLGGHS
                     FIGQLGNEPRPTEDTQCAIVEACLDNGIRWFDTTHRPERVALGKALQRLQRREEAVII
                     AWNFFDDVGIEPEDGLDRAAPYQRLHITQMLEQLQTSFIDCLVVHPVAGGDEVNAAQL
                     ELAFEWQKSGYVGVLGTWEPGEDAVERFGLENPYDFMVRPLNMATPDAGPAFAAAKRL
                     GWHTLAASPFVRGWHLDRLVAAARKSWRGSESDLRGRLADHMLRYSLFNPNVDRVITA
                     MRRVEWVTANVESVRRGPLTASELQWLDSISS"
     gene            2732..>4098
                     /locus_tag="P5179_11805"
     CDS             2732..>4098
                     /locus_tag="P5179_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS95946.1"
                     /translation="MSVRTPLTYRDRIEALRLTKLEQTRFKQSLGSRDTDDKGSVPLP
                     PDWQGFTPTPNHPSGAFFGPRACGENFRAMLESHPTYIDPMSSLAGGWMLSFSEYRKL
                     GWPPDPEFDYSHLHEDQRRYNVIPGIGGAQHFCPEVRIGLELGWGGLLEKVRRYRDLN
                     RPADTGFYDGLEDVVLGAQNWIGRHAHAARKAAEIETRPELRRNLLEMATMCERQVND
                     PPATFREACQWIAWFEMVACMYNNSGALGQIDEILRPYYERDMAAGTLTEEEAVWHLA
                     SLLIKETHYMQVGGPDPTGKKDLTSRVSFLVLEAAHLLRIPSNIAVRVHNKIDPALYD
                     TAVRYLFEDKTGSPLWMGSEALDEGFARNGYPIELARERVKCGCHWCALPGREYTLND
                     IVKINFAAVFDVALHDMIGNPSITPSVAELWRHFEKHLRRAVEVTAEGIDFHLAHYKD
                     VSPELV"
BASE COUNT          808 a         1255 c         1226 g          809 t
ORIGIN      
        1 gagacggcct tgagaagatt gagatgagcc gccgcgaaac gctgcgggtt ctcgtccgca
       61 tccctgaacc cccggaagca cgaagacaga atcttcaggg cggactcagg gtcgacttcc
      121 ttcagttgcc gtaacagctc gtagtcctca attcccgcgg cctgcgcttc gagtcgtacg
      181 ctgccccagg ggccgtcgtc cccgggatac acgttgtgag tgtcacccgg cggaaggttg
      241 ttcccgccgc cgtgatccgg gcagttgcac tcgaagggat tttgatcgtg gcgataatgg
      301 ttgaggcccc agtggaggaa ccctgggaga tcgtatttga aacaacccca ctgaatgtag
      361 cgcgtgcgga gaagcggcat gtcgagcagt cggttcatgt agtagccgcc ggggaagcaa
      421 caggtgtagc accatatcgt gtcgccgtga tgtcggaaga actcgaactc gtcgcggtgt
      481 ttctcgtagt agtcatcctt gggcacccag atatccacgg cgcccgcgag ttcagaaagc
      541 tccactgcgt cgatgatggg cgtacccggc atgaatttcc gtactatccc ggagatgacc
      601 cggtaatccg ccatggactt gtcggtgggc tcgtcgccca catgctggac ggcgatatcc
      661 agccagccgt tgcgacagag gaaatcccgc caggcaggga ggtattgcgc gaggaaattg
      721 tagccttcgc gggagacggc gttgactttg tggttggtgg aaagcacgaa gttgggcgcg
      781 tggaattccg tgcggccggc gacatgtcct ccctcgattc ccgtgaagcc cagtttcaaa
      841 taggtgcgga tcaagcgctc tgctttcgcg aaatcgaacg aaaagaggcc ttcaggtccc
      901 tctttgatac cgatgtaccc catcggaatc cagaaatgcg tctggcgccc ccgacgcatc
      961 agctcgccgt accgacgaat catccgccag tgggcttcgg tccacggcgt gattccatgg
     1021 cgggatgcca tggctccagg attgaaccaa ttggtgaggc tgaacgtgcc ggggtcagtt
     1081 accgtggccg cgtgaactgt gagtttgacg ggcagagtaa gcgactcctt cccgatcgta
     1141 acgcggatcg tgtccgtgta cgtggcgggg tcggcatcct cggggatgcg ccactcgaag
     1201 aacagcgctt cggagccctt tcttcccttg gtgcccggga gaaagggttt catggcgtcg
     1261 tacacccgga acggcgcgag gcgtgtggtg tacccttccg ctgattcgcc ttctttcgca
     1321 atgaaaccta tcggcccggt gttcttttcc accatgacat cgacgagctg gaagacctcg
     1381 ggcgagggga agtcccctcc ctgcgacgca aggctcacgt tcaattcgcc gtttgccggt
     1441 gtcggtttga agagaacctg aaaaccggtt cgcgttcccc gcgctgcatg cagaacgatg
     1501 ggtttgggcc gcgaaacccc ggccgccatg tccgggtaga gccactcgtt catactgaca
     1561 actctgcact tcatcgtctg cctcgtctgt tggaagtcaa taccggcgtc acgtctcaag
     1621 acgaaatcga gtcaagccat tgtagctcgg atgcggtcag agggccccgg cggacgcttt
     1681 ccacgttggc cgtgacccat tctacacgcc gcatcgccgt gatcacacga tcaacgttcg
     1741 ggttaaagag ggagtacctc aacatgtggt ccgccagccg gccccgaaga tcagactcac
     1801 tgccccgcca ggacttgcgg gcagccgcga cgagcctgtc gaggtgccat ccccgaacaa
     1861 aaggagacgc ggcgagagtg tgccagccaa ggcgcttcgc cgccgcgaat gcgggacccg
     1921 cgtccggagt cgccatgttc aaggggcgca ccatgaaatc gtacgggttt tccagtccaa
     1981 agcgttcgac ggcatcctcg cccggctccc acgttccaag gacgcccacg tagccgcttt
     2041 tctgccactc gaatgcaagc tccagttgcg ccgcgttgac ctcatctccc cccgccacgg
     2101 ggtggacgac gaggcagtca atgaacgagg tctggagttg ctcaagcatc tgggtgatat
     2161 gaagtcgttg gtacggtgca gcccgatcga gtccgtcttc tggctcaatg ccgacgtcat
     2221 cgaagaaatt ccaggcgata atcacggcct cttcccggcg ctgcagtctt tgaagggcct
     2281 ttcccagcgc gacacgctcc gggcgatgcg tcgtgtcaaa ccagcggatg ccgttgtcca
     2341 gacacgcttc aacgatcgcg cactgcgtgt cttccgtcgg acgcggttcg tttccgagct
     2401 ggccgatgaa actgtggccg ccaagaacaa ggggaacgtc gtcttcaaat gacgcgggcc
     2461 agccttgtga ggtccctttc gtcgaagcct ccctgttctt tgtttgtgcg tgcggcgttc
     2521 cagtgttttc gggcaacttc atcgcaatac ctccggggtg ccgcaataat gtactgacgc
     2581 ggcgcgagag aacaacccgt tcggaggccg ggctacgact tcgccgcgcc gaaaccggta
     2641 cgaaggagcg tacaattgca ccgcatttcc gtagtacgta tcgttcaaag gattcctctc
     2701 tactgcgcat caaatgaaag ggaaccccgt agtgagtgtg cgaacaccct tgacgtaccg
     2761 ggatcgcatc gaagcgctgc gcctgaccaa gctggaacag acccgattca agcagtctct
     2821 cgggtctcga gacacggatg acaaggggtc cgtcccgttg ccgccggact ggcagggatt
     2881 caccccgacg ccgaatcatc cttcgggtgc gttcttcggg ccgcgcgcgt gcggcgagaa
     2941 cttccgcgcc atgctcgaat cccacccgac ctacattgac cccatgagtt cgctcgccgg
     3001 cggttggatg ttgagtttct cggaatacag gaagctcggt tggccgcccg accccgaatt
     3061 cgactactcc cacctgcacg aagaccagcg ccggtacaac gtcatccccg ggattggagg
     3121 cgcgcagcac ttctgtccag aagtgcgaat cggtctcgaa ctcggctggg gtggactgct
     3181 tgagaaggtc cgccggtacc gcgacctcaa ccgtcctgcc gacaccggat tctacgacgg
     3241 gctggaagac gtcgtccttg gcgcgcagaa ttggattggg cgccacgctc atgcggcccg
     3301 caaagccgcc gagatcgaaa cgcgccccga gcttcgccgg aacctgctcg aaatggccac
     3361 catgtgcgag cgtcaggtca acgatccgcc cgccaccttc cgcgaggcgt gccagtggat
     3421 tgcctggttt gaaatggtcg cctgcatgta caacaacagc ggcgcactgg ggcagataga
     3481 cgaaatcctc cgtccgtact acgaacgcga catggcagcc ggcacgctca ccgaggagga
     3541 ggcggtctgg catctcgcgt cgctcctcat caaagaaact cattacatgc aggtcggcgg
     3601 gccggacccg accggtaaga aagatcttac cagtcgcgtc tccttccttg tgttggaggc
     3661 cgcgcatctg ctgcgcatcc cgtccaatat cgcggtgcgc gtgcacaaca aaatcgatcc
     3721 ggcgctgtac gacaccgcag ttcggtatct tttcgaggat aagaccggca gcccgctctg
     3781 gatgggctcc gaagcactcg atgaaggatt tgcccgcaac gggtacccga tcgagcttgc
     3841 gcgcgaacgc gtgaaatgcg gctgccactg gtgcgcgctc ccggggcgcg agtacaccct
     3901 gaacgacatc gtgaagatca acttcgccgc ggtgttcgat gtggcgctgc acgatatgat
     3961 cggaaatccc tcgatcacgc cttccgtcgc cgaactctgg cgtcatttcg agaagcacct
     4021 ccgccgcgcg gtggaagtga ccgcagaggg gatcgatttc cacctcgcgc attacaagga
     4081 cgtcagcccc gaactcgt
//
LOCUS       DAOTNG010000282         4088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17663_length_4088_cov_5.314654, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000282 DAOTNG010000000
VERSION     DAOTNG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17663_length_4088_cov_5.314654"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="P5179_11810"
     CDS             complement(<1..360)
                     /locus_tag="P5179_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRS95947.1"
                     /translation="MQHGESNARIEPRRRAPWRDKWYRIIFESDTPAGRLFDVVLMVA
                     IILSVLTVFVESVRSIRFRFGSVLFGLEWAFTLLFSVEFALRIATAKRPMRYVFSFFG
                     LVDILSILPTYLSLALPG"
     gene            complement(367..2202)
                     /locus_tag="P5179_11815"
     CDS             complement(367..2202)
                     /locus_tag="P5179_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRS95948.1"
                     /translation="MKPLAFAVGLQAMFSGGNLQSSSAVALAISICAVVCLLLLSILV
                     LSFGGRVRRFLAQTLVSKLLAEHPGEGRATPLWHRAVRATVETALWLVNVCASLALFY
                     FAASMALDLFDLQDAGLVYLQGSLISLLGLVVFFRINGSFPGVYRRLENWRSPLTIRG
                     HKLAEVETVADFLTFSSKALRVSFALVSFYVVGRYFTSLFPAGEAQASYEVVRGVMYA
                     VLATVLLAVVVRAINRFGAAVAARSRDWAAELEASPEETTLYVKMVHLGHTGIQILRV
                     MLLVLAVYGYAAFVLGLFAVTSTWVGSIVEMLVTPVRQVGDTVVGYLPNLFAIIVILA
                     VTYAIVKAVRWTASEIGKGTVTLPGFFPEWAAPTYKIVRFLIIVFCAVVLFPYLPGSG
                     SPAFQGISIFVGVLFSLGSSGAVSNIVSGVVLTYTRAFRVGDMVKISDTTGTVVEKTL
                     LVTRVRTVKNVDITIPNSMVLGSHITNFSSSGNTLIVHTSVTIGYDVPWRVVHELLLE
                     AADRSSSLLKNPPPFVHQTSLEDFYVEYELNAFTALPNTMAATYTELHQNIQDVFNER
                     GVEIMSPHYRAVRDGNQVAIPAEHVPEDYEAPRLRVETTQPHKKA"
     gene            2456..3271
                     /locus_tag="P5179_11820"
     CDS             2456..3271
                     /locus_tag="P5179_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS95949.1"
                     /translation="MNGEQVTFSVFTKPWKTLPLGELGALVRRFGFDAVELPVRPGYQ
                     VQPDTMETGLKEAVRAFADEGIVVKSVAAPTDGVRPGFTGELIAACAENDIPVIRVFP
                     FIRPDEPYLEGEARVQNELESLLPALEQCGVTVGVQNHCGPCFTGAMHLRHLIERFDP
                     QHIGAILDAGHTGLSGEEPEHAIDMVWSHLCMVNFKNAFRRLRTGPEAEQAEWQVYWT
                     SARHGLSDWRRYAAELKKRRWRGTTCLTAEYSDPNAVDRLIADDLRYTKSLFV"
     gene            3301..>4088
                     /locus_tag="P5179_11825"
     CDS             3301..>4088
                     /locus_tag="P5179_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS95950.1"
                     /translation="MGTDRPNIVFFLADDQGAWALGCAGNAEIRTPNLDRLAAEGIRF
                     TNFFCASPVCSPARASILTGRISSQHGIHDWIRAGNVPNEVPGVTHGRPVEYLRGMIG
                     YTDVLAENGYTCGLSGKWHLGDSLRPQKGFSFWNVHARGGSGYYDAPMIQDGIAYTEP
                     RYVTDVIAENAVAFLREQQATGTPFYLSVHYTAPHSPWTPDQHPRRTYDEYYQRYPFN
                     STPFEPMHPWQISSAPTGFDELSRRRELSGYYTAITEMDRTIGDV"
BASE COUNT          918 a         1181 c         1227 g          762 t
ORIGIN      
        1 gccgggaagc gccaggctga gatacgtggg caggatagag aggatatcaa cgagcccgaa
       61 gaaactgaac acatagcgca tcggcctctt cgccgtcgcg atccgcaggg cgaattccac
      121 cgagaaaaga agcgtgaatg cccattccag gccgaaaaga accgacccga atcggaaccg
      181 gatgctgcgg acgctctcca cgaacaccgt caaaacgctc agaatgatgg cgaccatcaa
      241 cacgacgtcg aacagccggc ccgctggcgt atcagattcg aagattatgc gataccattt
      301 gtcgcgccac ggagctcttc ttctgggctc gatccgtgcg ttcgactcac cgtgctgcat
      361 ctcgggtcaa gcctttttgt gcggctgtgt cgtttccacg cggagccttg gagcttcgta
      421 gtcctcaggc acatgctccg ctggtattgc cacctggttt ccgtctctca ccgcgcgata
      481 gtgaggcgac atgatctcca ctccccgctc gttgaacacg tcctggatgt tctggtggag
      541 ctcggtgtac gttgctgcca tggtgtttgg cagggcagta aacgcgttca actcgtactc
      601 tacgtagaaa tcctccaaag aggtctggtg tacgaagggt ggaggatttt tcagcaggct
      661 cgacgacctg tccgccgcct ccagaagaag ctcgtgaacg actctccacg gcacgtcgta
      721 gccgattgtc actgaggtgt gtactatcag tgtgttcccc gacgaactga aattcgtgat
      781 gtggctcccc agaaccatcg aattcggaat cgtaatgtcg acgtttttca ctgtccgaac
      841 ccgcgtaacg agcagcgtct tctccaccac cgtgcctgtc gtgtctgata ttttcaccat
      901 gtcccctacg cggaacgccc ttgtatacgt gaggaccaca ccggagacaa tgttggaaac
      961 agctccggaa gaaccgaggg aaaacaacac gccgacgaag atggatatgc cctgaaaagc
     1021 cggtgagccg gaccctggta agtaggggaa caggacgacg gcgcagaaaa cgatgataag
     1081 gaaacgaacg atcttgtacg tcggtgctgc ccactcggga aagaaccctg gcagggtcac
     1141 cgtgcctttg ccgatctctg aagccgtcca tctcacggct ttcactatgg cgtacgtcac
     1201 cgcaaggatg acgatgatgg cgaagagatt gggcaggtac ccaaccacgg tatccccaac
     1261 ctgacggacc ggagtgacca gcatctccac gatacttccc acccaggtgg aggtgactgc
     1321 gaacaagccc aacacgaagg cggcgtaccc gtaaacagca agtaccaaca gcatgacgcg
     1381 cagaatctgg ataccggtgt gcccgagatg aaccatcttc acgtaaaggg ttgtttcttc
     1441 ggggcttgcc tccaattcgg ccgcccaatc ccgcgaacgg gcggcgaccg ctgcgccaaa
     1501 gcggttgatc gcccgcacga ccacggcgag cagaaccgtg gccaacacgg catacatgac
     1561 accgcgcacg acttcatagg aggcctgggc ttctcctgcc gggaacaggg aggtgaaata
     1621 gcggccgacg acgtagaacg agacaagcgc gaaggagacg cgaagggcct tcgaactgaa
     1681 agtgagaaaa tccgctaccg tttcgacttc agcgagtttg tggcctcgaa tcgtcaacgg
     1741 cgagcgccaa ttctcgaggc gtcggtagac acctgggaag gatccgttga tccggaaaaa
     1801 cacgacaagg ccaagcaggg agatgagaga cccctgcagg tagacgagtc ccgcgtcttg
     1861 aaggtcgaac aaatcgagag ccatgctggc agcgaagtag aacagcgcaa gggaggcgca
     1921 gacgttgacc agccacaacg ccgtttctac ggttgctcgc accgccctgt gccatagtgg
     1981 tgtcgcgcgg ccctcgccgg ggtgctccgc caagagcttc gagacgagtg tctgtgccag
     2041 aaaacggcgt acccgcccgc caaacgagag gacgagaata cttaacagca aaaggcagac
     2101 gacggcgcag atggaaatcg ccagggcgac cgcgctgctc gattggaggt tgcctcccga
     2161 gaacatcgcc tgcagaccga cagcaaacgc caacggtttc atccgcattt ccttccgtga
     2221 gtaaggcacc tggccgggcg gaagcgctgt atggtacgca atatacgcaa cgggaggccg
     2281 gccacgcatc cgccgagccg agcggagtag cagcggtcgc cccttctacg gccggtgcca
     2341 accaccacgg gctggcagtg cccttgtgtt actccggtgg gtgcgcatat catagcggtg
     2401 tgatgcagag tagcggatct gtttcgcatg cgcagcttcg tgggagggcc cggacatgaa
     2461 cggtgagcaa gtaacgtttt cggtattcac caagccgtgg aagacactgc cgctcggtga
     2521 actgggagcg ttggtaaggc ggttcggatt cgatgcggtg gagctccccg ttcgacccgg
     2581 ctatcaggtg cagccggaca ccatggaaac cggcttgaaa gaagcagttc gggcatttgc
     2641 ggatgaggga atagtggtaa aaagcgtcgc ggcgccgact gacggcgtac gccccggctt
     2701 caccggggag cttatcgcgg cgtgcgccga gaatgatatt ccggtgattc gcgtgttccc
     2761 gttcatccgc cccgatgaac cctatctgga gggcgaggcg cgcgttcaga atgaactgga
     2821 aagccttctg ccggcgctcg aacaatgtgg ggtgacagtt ggcgttcaga accactgtgg
     2881 accgtgtttc accggcgcga tgcatctccg ccacctgata gaacgttttg acccgcaaca
     2941 tatcggagcg attctcgatg ccgggcacac cggtctttcc ggcgaggaac ccgaacatgc
     3001 gatcgacatg gtctggtcgc atctctgcat ggtgaatttc aaaaatgcat tccgccgact
     3061 gcgaaccggg ccggaagcgg aacaggcaga gtggcaggtg tactggacca gtgcccgcca
     3121 cgggttgtcc gactggagac ggtacgccgc ggaactgaaa aagcggcgat ggcggggaac
     3181 aacgtgcctc acggcggaat actcggatcc gaacgcggtg gaccgcctta tcgcagacga
     3241 tctgaggtac acgaagtccc tttttgtcta acgccggcta tcagtcggaa gggggatgcc
     3301 atgggaacag atcgtccgaa catcgtgttt tttctcgcgg atgatcaggg cgcatgggcg
     3361 ctcgggtgcg caggtaacgc ggaaatcagg accccgaacc ttgatcggct tgccgcggaa
     3421 ggtatccgat tcacgaactt cttctgcgcg tctccggtct gttcgcctgc ccgagccagc
     3481 atcctcaccg gtagaatttc ctcccagcac ggcattcatg actggatacg cgcgggaaat
     3541 gtgccgaacg aagtgcccgg agtgacgcac ggaaggcctg ttgaatacct tcgcggcatg
     3601 atcgggtaca cggatgtcct tgcagaaaac gggtatacgt gcggcctgag cgggaaatgg
     3661 cacctcgggg attcccttcg cccgcagaag gggttctcct tctggaatgt ccacgcgcgc
     3721 ggcgggagcg gatattatga tgctccgatg attcaggacg gtatcgcgta cacagaaccg
     3781 cgatatgtga ccgacgtcat tgcggagaac gccgttgcgt ttctgcggga gcagcaggca
     3841 acggggacgc cgttctatct cagcgttcat tacaccgctc cgcacagccc gtggactccg
     3901 gatcagcacc cacggcggac gtacgatgag tattaccaac gctacccttt caattcgacg
     3961 cccttcgaac cgatgcaccc ctggcagatt tccagcgcgc ctactggctt cgacgaactg
     4021 agccgccgcc gcgaactgag tgggtattac accgccatca ccgagatgga ccgcactatc
     4081 ggcgatgt
//
LOCUS       DAOTNG010000283         4083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17687_length_4083_cov_5.639523, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000283 DAOTNG010000000
VERSION     DAOTNG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17687_length_4083_cov_5.639523"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="P5179_11830"
     CDS             <1..1103
                     /locus_tag="P5179_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS95951.1"
                     /translation="YGNEPNQWGNNVGDWHDGAWMKEGGGLKAVADYVHEKGLKFGLW
                     VEIEAAGANSTLKKEHPDWLMTRDGEPVAGGRALDLTKPEVAEFVENEIARLIRTYDL
                     DMFRIDHNHCIQPAGNRRVHGFTEDLIWRYYDVLYGIFDRLRARFPNVVFQNCAGGGG
                     RLDWGTLARFHNTELSDWMRMPRGLKILNGVTMSLPPEILLRTFGTEVGEHALEGDVH
                     TQLRLCFCRPIFRGIAPSMDALTPCLRDRITHYLEVFRKHIRPIMRDGLVFHHTPFLS
                     HQEITPWCVLEYASPDRDRAVAAVFRTSAEGPGEYLFRPRGLDLSTTYRVVLDNRNQS
                     FRATGRDLVTDGVRLQIDRTLASELIVCEAVR"
     gene            1139..2743
                     /locus_tag="P5179_11835"
     CDS             1139..2743
                     /locus_tag="P5179_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95952.1"
                     /translation="MQIYAPFTVDGVPPLGTYGTGSLAELAELKECGMNLILGGHAQV
                     DLSTPEGKFCHENGIRVMYHLTQHLYGNPQLAESVDATQTTIPLARVPATRRDAHLIE
                     IEGERIRYREMTDSALVGCERGVDGTTPAPHRKGIILFWPEPLEAEVARVKDSPNLWG
                     YYVLDDSPGDAISALRGMYRGIKRMDTANHPVCAGYGGAAAICNFGPGVCDMMLIYFY
                     PCMEGGYVRSFVTQETQWLLSRARERVPGIPFIGVYQGFWGIAGYPDIPVTPHQLREQ
                     MEDFVRDGASGLVAFTARSKAHGDTFDGWNARADLREEMRRINEEIRTTGGLSVPPEP
                     PELASLRIQPTGFWEKPLPIPGLVTAWHVIGPFKDVEKKILDAVFPPEREINLDATYE
                     SAKGPLRWQVFSHEDVYQLNGVLGGDPIYTEEWQQELMNVTAYLTCTVTSPREQEVVL
                     RVGSDDDAILWINGREVWRHDGERGILRDQDTARATLAKGRNVFLMKLYNRRDMWGAS
                     LRITDEGGRAIDGVTISPTADDIAPA"
     gene            2776..3510
                     /locus_tag="P5179_11840"
     CDS             2776..3510
                     /locus_tag="P5179_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95953.1"
                     /translation="MPVTDFTNSYMTFTVPNGENTARIQLDALCRVTEAGTTREAVLI
                     TPCKSELMYGQTKLFQDPNYDFCGIWTSDEYVIFRTYARHLPYREREAEAGSNLPRFD
                     EVRIDIRSFPKAAPLATPGDVVDATLANRHIVARSKISSTDGKSVAELEYPVKTMNVM
                     QAKRWFQVDTGPVIIPAWAYGGTPRGNRFVEQFTMAFVCFNVMEGYTEFVLREPTPVP
                     GNEAALVWHYSRIVNVPARHEFWSVG"
     gene            3642..>4083
                     /locus_tag="P5179_11845"
     CDS             3642..>4083
                     /locus_tag="P5179_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95954.1"
                     /translation="MSVRFDNARWERIRGLYSDWWAGKIDRPLFHIAVSGYDPGRPEP
                     ALRRHPIEPCADLSIPAEEVVDAWDWEVSRWEWLGDAFPIAWPNLGPGVLAAMLGARL
                     HATPETIWYEIPAEWGISGKPIEEIDLFWNPENPWVRRCEDLMRA"
BASE COUNT          798 a         1254 c         1322 g          709 t
ORIGIN      
        1 ggtacggcaa cgagccgaac cagtggggaa acaacgtcgg ggactggcat gacggcgcgt
       61 ggatgaaaga aggcggcggc ctgaaggcgg tagcggatta tgtccacgaa aagggtttga
      121 aattcgggtt gtgggtggaa attgaagcgg cgggagcgaa cagcacgctc aagaaggagc
      181 atccggactg gctgatgacg cgggatggcg aaccggttgc gggagggcgg gcgctggacc
      241 tgacgaagcc ggaagttgct gagtttgtcg aaaacgagat cgcccggctg atccgcacgt
      301 acgacctcga catgttccgc atagaccaca accattgcat ccagcctgca gggaaccgcc
      361 gcgtgcacgg gttcaccgag gacctgatct ggcggtacta cgatgtgctg tacggaatct
      421 tcgaccgcct ccgcgcacgg ttcccgaacg tcgtattcca gaactgcgcc ggaggcggcg
      481 gacggctcga ctgggggacg ctggcgcggt tccacaacac agagctctcc gactggatgc
      541 gcatgccgcg cggcctgaag atcctgaatg gcgtaacaat gtcgcttcct ccggagatac
      601 tcctgcgtac gttcggcacg gaagtgggtg agcatgcgct cgagggtgac gtgcataccc
      661 agctccggct ctgcttctgc cgccccatct tccgcggcat cgcgccatcg atggatgccc
      721 tgactccctg cctccgcgac cgcatcaccc actacctcga ggtattccgg aagcacatcc
      781 ggccaatcat gcgggatgga ctggtatttc accatacgcc gttcctttca caccaggaaa
      841 taaccccgtg gtgtgtgctg gagtatgcgt cgccggaccg cgaccgcgcc gtcgcggcag
      901 tgttcaggac gtccgccgaa gggccgggag agtacctctt ccgcccgcgc gggctcgatc
      961 tttcaaccac gtaccgcgtc gtgctggata accgcaacca gtcgttccgg gcgacgggca
     1021 gagacctggt caccgacggc gtccggctgc aaatcgaccg cacgctcgcg tcagaattga
     1081 tcgtatgtga ggctgttcgt taaagaggcg taccaccacc tcggaaggga atccagacat
     1141 gcagatttac gcgccgttca cggttgatgg cgttccaccc ctcggcacct acggcaccgg
     1201 ttccctcgcg gaactggcgg agttgaaaga atgcggcatg aacctgattc tgggggggca
     1261 tgcgcaggtt gatctcagca cgccggaagg gaagttctgt cacgagaacg gcattcgggt
     1321 gatgtaccac ctgacacagc atctgtacgg caacccacag cttgccgagt cggtggacgc
     1381 gacgcagacc accatcccgc tggcgcgtgt accggcaacg cggcgtgatg cgcacctcat
     1441 cgagatcgaa ggagaacgaa tccgctaccg ggagatgacg gactcggcgc ttgtgggctg
     1501 cgaacggggc gttgacggca ccacgccggc tccccaccgg aaggggatca tcctcttctg
     1561 gccggagccg ctggaggcgg aggtggcgcg ggtgaaggac tccccgaacc tctggggcta
     1621 ctatgtgctg gatgacagcc ccggagacgc gatctcagcg ctgcgcggga tgtacagggg
     1681 catcaagcgc atggacaccg cgaaccatcc ggtctgcgcg ggatatggcg gcgcagcggc
     1741 catctgcaac ttcggtcccg gcgtgtgcga catgatgctg atttacttct acccgtgcat
     1801 ggaaggcggg tacgtcaggt cgttcgtcac tcaggagacg caatggcttc tcagccgcgc
     1861 gcgggagcgt gttccgggta tcccgttcat cggcgtgtat caggggttct ggggcatcgc
     1921 cggctatccc gatatccccg ttaccccgca tcagctccgc gagcagatgg aggatttcgt
     1981 ccgggatggc gcctcgggcc tcgtggcgtt cacggcccgt tcgaaagcgc acggcgacac
     2041 gttcgacggg tggaacgcgc gggcggacct tcgtgaggag atgcgcagga tcaacgagga
     2101 gatccgcacc accggaggac tctcggttcc gccggagccg ccggaacttg ccagccttcg
     2161 tatccagccc accgggttct gggaaaaacc cctcccgatc cccggcctcg tcaccgcgtg
     2221 gcacgtgatc gggcccttca aggacgtaga aaagaagatt ctggacgcgg tgttcccccc
     2281 ggagcgcgaa atcaaccttg acgcgacgta cgaaagcgcg aaaggaccgc ttcggtggca
     2341 ggtcttctcg cacgaggatg tctaccagct caatggtgta cttggcggcg atccgatcta
     2401 caccgaggaa tggcaacagg agctgatgaa cgtaaccgcc tacctgacat gcacggtgac
     2461 gagcccgcgc gaacaggagg tcgttctccg cgtcggcagc gacgacgacg cgatcctgtg
     2521 gatcaatggc cgcgaggtct ggcgccacga cggggagcgg ggcatcctgc gcgatcagga
     2581 taccgcgcgg gcaacactcg cgaagggacg gaacgtgttc ctgatgaagc tgtacaatcg
     2641 ccgcgatatg tggggcgcgt cgctccgaat cacggatgaa ggcggccggg cgattgacgg
     2701 cgtgacgatc agccccactg cagacgacat tgcaccggcg tgacccatcc agttctcctg
     2761 aaggaggatt cgcccatgcc agtcacggac ttcaccaact cgtacatgac gttcactgtc
     2821 ccgaacggcg aaaacaccgc ccgcatccag cttgacgccc tctgccgcgt taccgaagcg
     2881 gggacgacac gggaagccgt gctgatcacc ccgtgcaagt cggaactcat gtacggccag
     2941 acgaagctgt ttcaggaccc gaactacgac ttctgcggga tctggacctc tgacgagtac
     3001 gtcattttcc gcacgtacgc ccgtcatctt ccctatcggg agcgcgaggc cgaagccggg
     3061 tccaacctcc cgcgcttcga cgaggttcgg attgatatcc gaagcttccc gaaggccgcc
     3121 cccctcgcaa cccccggcga cgtggtggac gccacactgg ccaaccggca catcgttgcg
     3181 cggagcaaga tctcaagcac cgacgggaag agcgtcgcgg agcttgaata cccggtcaaa
     3241 accatgaacg tgatgcaggc gaagcgctgg tttcaggtgg atacgggccc ggtcatcatc
     3301 ccggcgtggg cgtacggcgg cacgccccgg ggcaaccgct tcgtggagca gttcaccatg
     3361 gcgttcgtct gtttcaacgt gatggagggc tacaccgaat tcgtcctgcg cgagccgacg
     3421 cctgttccgg ggaacgaggc ggcgctggtg tggcactaca gccgcatagt gaatgtaccg
     3481 gcgcggcacg agttctggtc tgtcgggtaa cgtcgagccg ttgcacgagc gtctcgccgg
     3541 tgccgcttcc ccatgcaccg attctattcc gtcattcccg cgaaggcggg aatccagaaa
     3601 aacgcctcct gtagagaaca gcccagggag aagcaactcc gatgtccgta cgttttgaca
     3661 acgcccggtg ggagagaatc cgcggccttt acagcgactg gtgggcaggc aagatcgacc
     3721 gcccgctctt tcacattgcg gtgagcgggt acgacccggg ccgcccggaa cctgcacttc
     3781 gccggcaccc gatcgagccc tgtgccgacc tctccattcc ggcagaggag gtggtggacg
     3841 cgtgggactg ggaggtatcc cggtgggagt ggcttggcga cgccttcccc attgcgtggc
     3901 cgaacctcgg tcccggagtg ctggcggcaa tgctgggggc acgtctgcac gccacgccgg
     3961 aaacgatctg gtacgaaatc cctgccgagt ggggtatttc cggcaaaccg atagaagaga
     4021 ttgacctgtt ctggaacccg gagaacccgt gggtgcggcg ttgcgaggat ctgatgcgcg
     4081 ccg
//
LOCUS       DAOTNG010000284         4061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17793_length_4061_cov_3.302047, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000284 DAOTNG010000000
VERSION     DAOTNG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17793_length_4061_cov_3.302047"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5179_11850"
     CDS             complement(<1..664)
                     /locus_tag="P5179_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HRS95955.1"
                     /translation="MSTLLAKKVQSGYACALNNERVDVAASRKAAFGREPAAGGLRDV
                     QDRLRALRGTLGYRRFAARVGAKPNSVRRYELGMARVPLDYVQAVCRAFNVSADWLLF
                     GIEAPAGLADAAVRAIREGRLPATRSAIVAGERVRIVCVPVLNRVPAGAPAEMLDDMP
                     VGFGLEGVIRVPDPGDANAYALTAWGDSMEPLIRDGERIVVSPRRAQGFSAGIAVVRL
                     RSQ"
     gene            770..1546
                     /locus_tag="P5179_11855"
     CDS             770..1546
                     /locus_tag="P5179_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95956.1"
                     /translation="MTRHPVDPLETHEHECPELPGVCRDILTAPMALDRLLRPDFPAP
                     GDMPEEACADAEAELDSIEAVLSSAEADFEWKEPASDSAGAGRGRWGDLSRIRRIPLA
                     DYRPKEVVPDEVLTRQNADRGRPHPIRDRPAPEPDTEVEPRGPGRVWELRDRLRECAM
                     CGGLFIPSRFNHRFCSRWCAWKSRVHVCVCPACGVEFVAERPEQVWCSVACGASSRRT
                     TEYDHICARCGNPYRATRATQRFCSRACARASRKRTRRAG"
     gene            complement(1892..2122)
                     /locus_tag="P5179_11860"
     CDS             complement(1892..2122)
                     /locus_tag="P5179_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRS95957.1"
                     /translation="MPKLPRVTARELIGAFERAGYVVRRQSGSHVVLRHPVTGQILVV
                     PDHHGILGTGLVHSLLRHAKLSVADLVRLLGR"
     gene            complement(2115..2393)
                     /locus_tag="P5179_11865"
     CDS             complement(2115..2393)
                     /locus_tag="P5179_11865"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRS95958.1"
                     /translation="MKRTYTVMLIPDPDGGYVVEVPALPGCVSFGDSLSNALDMASDA
                     ILNYLFVLQSDGEAIPREGPQIVVSLGDNREAILRKVVVETEEVSQVA"
     gene            complement(2694..3413)
                     /locus_tag="P5179_11870"
     CDS             complement(2694..3413)
                     /locus_tag="P5179_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS95959.1"
                     /translation="MPAPWYEQFFGHDYARFDRHPDTGREIAFLQQVLPEPPARVLDL
                     ACGTGRHAAPMSRAGYDVVGLDLSPVLLSRARKKRSTAQWLRADMSRIPLRDGSCDAV
                     ISLFSSIGYFDDESDNYRVLSEAARVLRNGGRLVIETVNRDFFIRHAPPQSWFWQGAL
                     TVLEERRMDPLTSRSEVDVVVLEGGRHRVYHHSIRLYTAAELATLLASAGVEVLDVFG
                     DYDGAALSFDTARMVVVGERR"
BASE COUNT          714 a         1372 c         1313 g          662 t
ORIGIN      
        1 cctgcgaacg cagcctcacg acggcaatcc cggcggaaaa cccctgcgcc cgccgcggac
       61 tgacaacgat ccgctcgccg tcgcggatca gcggctccat ggaatccccc cacgccgtca
      121 gcgcatacgc gttcgcatcg cctgggtccg gcacccggat gaccccctcc agcccgaacc
      181 ccaccggcat gtcatccagc atctccgccg gagcgcccgc cggcacgcgg ttcagcaccg
      241 gcacgcagac aatccgcacc cgctcccccg caacgatcgc gctccgcgtc gcgggcagac
      301 gcccctcccg gatcgcccgc accgccgcgt ccgccaaacc cgccggcgcc tcgatgccga
      361 acagcagcca gtccgccgat acgttgaacg ccctgcacac ggcctgaacg taatccagcg
      421 gcacccgcgc catccccagc tcataccggc gcaccgagtt cggtttcgcg cccacccgcg
      481 cggcgaatcg ccggtatccc agcgtgcccc gcagcgcccg caggcggtcc tgcacatccc
      541 ggaggccgcc cgctgccggt tcacggccga acgctgcctt gcgggaggcg gcaacgtcca
      601 cccgctcgtt gttcagggcg caggcgtacc ccgattgaac ttttttcgcc aaaagtgttg
      661 acacgaagac gcctcctttc cagtgttgat catacacgta aatatgcggg tagttttgcc
      721 cggacaaaag tgccatgacc aggtgacatt gtgaaaggag aaaacctgaa tgacgcgcca
      781 ccccgttgat ccgctggaaa cgcacgaaca cgaatgcccc gaactgccgg gggtctgccg
      841 cgatatcctt accgccccca tggcgctgga ccggttgctg cggcccgatt tcccggcgcc
      901 gggcgatatg ccggaggagg cctgcgcgga tgcggaggca gagctggatt ccatcgaggc
      961 ggtcctctcg tcggccgagg cggatttcga gtggaaggaa cccgccagcg actccgcagg
     1021 ggccgggaga ggtcggtggg gggatctctc ccgcatccgg cgaatcccgc tcgccgacta
     1081 tcgccccaaa gaggtcgttc cggatgaggt tctgacccgg cagaacgcgg acagggggcg
     1141 cccccatccg atccgcgacc gccccgcgcc ggagccggat accgaagtcg aaccgcgggg
     1201 acccggacgg gtctgggagc tgcgcgatcg tctgagggag tgcgcgatgt gtggaggcct
     1261 tttcatcccc agccggttta accatcggtt ttgttcgcgg tggtgcgcat ggaaaagccg
     1321 ggttcacgtc tgcgtctgcc cggcgtgcgg cgtcgagttc gtcgcagagc ggcctgagca
     1381 ggtctggtgt tccgtcgcct gcggtgcttc ctcccgccgc accacggagt acgaccacat
     1441 ctgcgcccgg tgcgggaatc cgtaccgcgc cacccgggcc acgcagcgtt tctgcagccg
     1501 cgcctgcgcc cgtgcctccc ggaaacgcac gcgccgtgcc gggtagccgc cggggaggga
     1561 tacgctccgt cgggtcctgc gcgggttatc gaggctgcgc cccgtgccgg tcgtcgagtg
     1621 gcgaaccggt ccctgagcct gcgcggccaa aacaccgtca ttcccgcgca ggcgggaatc
     1681 cagtgacgcc tgaaccaaca acatgaatga acacgcggga acgcccggcg ttctcagtcg
     1741 tgtctggcgg cgggtcactg agccagtcga agtgccggtt cgttgtatcg agacgaccgt
     1801 gcctgttctg cgggcacgga gggtcgcgct ccgtcgctac ctgcgcagga tctctcgtgt
     1861 gcccaacggt cttgagggcg cgaggggagg gtcaacgccc gagaaggcgg acaaggtcgg
     1921 cgacgctgag tttcgcatgc ctgagaaggg aatgcacaag ccccgtgccg aggattccgt
     1981 ggtgatccgg caccacaagg atctgcccgg tgaccggatg cctcagcacg acatggctgc
     2041 cgctttgcct gcgcacgaca tatccggcac gttcaaaagc gccgataagc tcgcgtgcgg
     2101 tcacgcgagg aagcttaggc aacctggctt acctcttccg tttccacgac gactttgcgc
     2161 aggatcgcct cgcggttatc cccgaggctc acgacgattt gagggccttc tctggggatc
     2221 gcctcgccgt cactctggag cacgaaaagg tagttaagaa tggcatccga ggccatatcg
     2281 agggcgttgg agagggagtc accgaaggac acacaacccg gaagcgccgg aacctcaacc
     2341 acgtacccgc cgtccgggtc cggtatcagc ataaccgtat acgttcgttt catcggatgc
     2401 ctttttcaaa acggccgcag gcggacattt cgagagagat gatagacccc gggagcgcgc
     2461 atgtcaagga acacaggaga cgtcgaccgg ctgagtctgc tcacgccccg ctgggagccc
     2521 ggaacagggc cgcggactcc cctggaggga tgcgctccgt cgggtcctgc gcgggttacc
     2581 gaggctgcac cccgcgccgg tcgtcgagtg gcgaaccggt ccctgagcct gctgcctgtg
     2641 cgggcagcgt gtcgaagggc cggttcgctg tatcgagacg accagccccc gggttaccgc
     2701 ctctccccga ccaccaccat gcgcgcggtg tcgaaggaaa gggccgcgcc gtcgtaatca
     2761 ccgaacacgt cgagcacctc cacccccgcg gaggcgagca acgtcgccag ttccgccgcg
     2821 gtgtagagcc ggatcgagtg gtggtagacg cggtggcggc cgccctccag caccaccaca
     2881 tccacttcgg agcggctcgt aagcgggtcc atccgccgct cctccagcac ggtcagggcg
     2941 ccctgccaga accacgactg cggcggcgcg tgccggatga agaaatcgcg gttgacggtt
     3001 tcgatgacga gccggccgcc gttccgcagc acccgcgccg cctcgctgag tacgcggtag
     3061 ttgtccgatt cgtcgtcgaa gtatcctatg ctgctgaaca gggaaatcac cgcatcgcac
     3121 gagccatcgc ggagggggat gcgcgacata tccgcgcgaa gccactgcgc ggtcgaacgc
     3181 tttttccggg cgcgggaaag caggacgggg gaaaggtcca gcccgaccac gtcgtacccc
     3241 gcgcgcgaca ttggagccgc atgccgcccg gtgccgcacg cgaggtccag cacccgtgcg
     3301 ggaggctccg gcagtacctg ttgcaggaac gcgatttccc gcccggtgtc agggtgccgg
     3361 tcgaaccggg cgtagtcgtg cccgaagaat tgctcgtacc atggtgccgg catggcgtgg
     3421 tcctttcgcg attaaccgtt tgttgttccg tctcaacggt accacggtcg tcggcgcggg
     3481 gggaagacgt ggcggggccg gtgcggcaag acgttcctgc ctgcctgagg gtggggcgga
     3541 cattcttgtc tgccttggtg gagcactgct gtatgggcgc cacgagattc ttcgcttcgc
     3601 tcagaaagac gatcgttccg ggggctgcgg gtaccatgcc gtcattctga gcggcgtcca
     3661 cgaaacggac gacagcgaag aatctcctgc ccaaaacaca ctcctgctcc ctcgcagacg
     3721 acatcagccg gaacggcagc aaagggaaac gtcattccgc gtggtgaaca gagcacgcgg
     3781 aatgacggct ctgtgcggtg cggcgggttc agtggggcgg agtgccctgc cagggggcag
     3841 ttggcgcagc cccatgagat ccttcgcttc gctcaggatg accgcttcag acgcacgcta
     3901 cgcggccgcc gctcaggatg acggttttct tgagggcctc cgaaatctgg ggcgtcattc
     3961 tgagcgaagc gaagaatctc acgattccgc gagcgcacga gaagccatcg cgagcgcgct
     4021 cccgaagccg ctcttgacat cgcacgggcg cttttcctat t
//
LOCUS       DAOTNG010000285         4033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17948_length_4033_cov_5.086727, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000285 DAOTNG010000000
VERSION     DAOTNG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17948_length_4033_cov_5.086727"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..579)
                     /locus_tag="P5179_11875"
     CDS             complement(46..579)
                     /locus_tag="P5179_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRS95960.1"
                     /translation="MPDGTRSGNKISRGNAAQFFVAGELCRRGHAAVVTLGNTPNVDI
                     LCSNAAGTRFAHIQVKTFEPGNRTCSVGIKAETDFGSTFFWVLSGIPSFGSDREFEYY
                     IIPSREMARNVREGHEKWLATPGKNGQVHHDSNVRAVNLPPFSPFWGWDITSFRNRWD
                     LIEEVLKPVSGFPPSRE"
     gene            complement(827..3574)
                     /locus_tag="P5179_11880"
     CDS             complement(827..3574)
                     /locus_tag="P5179_11880"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRS95961.1"
                     /translation="MSVSPNPDATATDTAQAPHQFRISRYFSRPGTHPFDEIEWDIRT
                     ATIGNEKGETVFEQRDVEVPKSWSQLATNVVVSKYFHGPKGSPQRERSARQLVTRVVD
                     TITEWGDKDGYFLDEEERETFRAELTAILINQRASFNSPVWFNIGVPGARPQASACFI
                     NSVRDEMESILDLAKTEGMLFKWGSGTGTNLSPIRSSREALSSGGTASGPVSFMRGYD
                     TFAGSIKSGGKTRRAAKMVILNIDHPDIREFILCKVEEEKKARILIEAGYDPGFNVPG
                     GAYDSVHFQNANNSVRVTDEFMRAVENDGEWQTHAVVGGRVMDTYRARDLMQWIAESA
                     HMCGDPGIQYDTTINNWHTCPNTDRIHASNPCSEYMFLNDSACNLASLNLMAFRDENG
                     EFDIRAFRHAIRVVITAQEILVGNASYPTPAIERNSHDYRPLGLGYANLGALLMARGL
                     PYDSDEGRAYAGAVTALMTGEAYRTSAHLAARKGPFAGFAANREAMLGVIRKHGDHVQ
                     RIDRSYVPEPLFRAAETSWREAYELGERHGFRNAQATVLAPTGTIGFMMDCDTTGIEP
                     DMALVKYKKLVGGGALKIVNTTVPLALATLGYTETEIRQIVEYIDKNDTIEGAPFIKD
                     EHLPVFDCAFKPVKGTRSIHYMGHVRMMSAVQPFISGAISKTVNMPETATVEEVRETY
                     MQGWKLGLKAIAIYRDNCKSTQPVSTKRTEKAAPAEPEWKPRRRRLPDERNAMTHKFS
                     IAGHEGYITVGMYEDGQPGEIFIVMSKVGSTIAGLMDAFATMVSLALQYGVPLKVLAD
                     KFSHSRFEPAGFTNNPQIPMAKSIMDYIFRWLALKFLPQEERPTNGDNGHNGDKPPIA
                     EDLSASTLEIRRKAAIEAAEKAVFVQQGDAPLCPDCGSIMIRSGACYKCLNCGGTSGC
                     S"
BASE COUNT          732 a         1222 c         1249 g          830 t
ORIGIN      
        1 gcgcaatcca gagacaaacc cgtgtgcaag atgagaagac tgccgtcatt cccgcgaagg
       61 cgggaatcca gacacaggtt tcagcacctc ttcgataagg tcccaccggt tacgaaacga
      121 agtgatgtcc caaccccaga acgggctgaa cggtggcaga ttcaccgccc gtacgttgct
      181 gtcgtggtgg acttgaccgt tctttccagg cgtggcgagc catttctcgt gcccttcgcg
      241 cacgttccgc gccatctcgc gtgaaggaat gatgtaatac tcgaattccc ggtcagagcc
      301 gaaagagggg atgccgctca aaacccagaa gaatgtggag ccgaagtcgg tttcggcttt
      361 gatccccacg ctgcaggtgc ggtttcctgg ctcaaacgtc ttcacctgta tgtgggcaaa
      421 acgggttcca gcagcattgc tgcacagaat atccacattg ggcgtattgc cgagcgtaac
      481 caccgctgcg tggccgcgcc ggcagagctc ccccgcaaca aagaactggg cggcgttccc
      541 gcgggaaatt ttgttgccac tccttgtgcc atcgggcatc cgaaacctcc cccactgatg
      601 actgtaaatg cgacccttcg gtcgtcccgg cgagttttct ttcccatgac aggcaggaat
      661 gcctgtccca cctgtccaat cgtcacaccg agaagtacgg cggcctgcgt gtgtaaacgt
      721 gcgcgggcaa cgacaacctc aggacgcgca ccccccaaaa cgcgcgtagg ggcggttcgc
      781 gaaccgcccc tacacccgaa aacaccgatt tccaggatgt ttccccctac gaacacccgc
      841 tcgttccgcc gcagttgagg cacttgtagc acgctccgga acggatcatg atggagccgc
      901 aatcggggca gagcggggcg tcgccctgct ggacgaacac cgccttctcg gccgcttcga
      961 tggccgcttt ccgccggatc tcgagcgtgc ttgcgctcag gtcctcggcg atgggcggct
     1021 tatcaccgtt gtggccgtta tccccgttgg tcgggcgctc ttcctgcggc aggaacttga
     1081 gcgcgagcca gcggaagatg tagtccatga tgctcttcgc catgggtatc tgcgggttgt
     1141 tggtaaagcc cgccggttcg aagcgcgaat ggctgaactt atccgccagc accttgagcg
     1201 gcacgccgta ctgcagcgca agcgacacca tggtggcgaa ggcgtccatc agcccggcga
     1261 tggtggagcc gaccttcgac atgacgatga agatttcgcc cggctggccg tcctcgtaca
     1321 tgccgacggt gatgtagccc tcgtgcccgg cgatgctgaa cttgtgcgtc atggcgttgc
     1381 gttcgtcggg aagccggcgt cggcgaggtt tccactctgg ctccgccggc gcggcctttt
     1441 ccgttctctt cgtgcttacc ggctgcgtgc tcttgcagtt atcgcggtag atggcgatcg
     1501 ccttgaggcc gagtttccag ccctgcatgt aggtttcccg cacctcttcc accgtcgccg
     1561 tttcaggcat gttgacggtt ttcgagatcg ccccggagat gaacggctgc accgcgctca
     1621 tcatgcggac gtggcccatg tagtgaattg accgggtgcc tttcaccggc ttgaacgcgc
     1681 agtcgaacac cggcaggtgc tcgtccttga tgaacggcgc gccttcaatc gtgtcgttct
     1741 tgtcaatgta ttcgacgatc tgccggattt ccgtctcggt gtacccgagg gtggccagcg
     1801 caagcggcac cgtggtgttg acgatcttca gcgcgccgcc gccgacgagt ttcttgtatt
     1861 tgacgagcgc catgtcgggc tcgatgccgg tggtgtcgca gtccatcatg aagccgatgg
     1921 tgccggtggg ggcgagcacg gtcgcctgcg cgttgcggaa cccgtgtcgt tcgcccagct
     1981 catacgcctc gcgccaggag gtttcggcgg cgcggaagag cggctccggc acgtagcttc
     2041 tgtcgatgcg ctgtacgtgg tcgccgtgtt tcctgatcac gcccagcatg gcctcccggt
     2101 tggcggcaaa gccggcgaac gggcctttcc gcgcggcaag atgggcggag gtgcggtagg
     2161 cttctcccgt catgagcgcg gtaacggccc cggcgtaagc gcgtccttcg tcgctgtcgt
     2221 acggcaggcc ccgtgccatg aggagcgcgc cgaggttggc atatcccagc ccgagcgggc
     2281 ggtaatcgtg gctgtttcgc tcgatggcgg gcgtggggta gctcgcgttg ccgacaagga
     2341 tctcctgcgc cgtaatcacc acccgaatgg cgtggcggaa cgcgcggatg tcgaactccc
     2401 cgttctcgtc acggaacgcc atgaggttaa gcgatgcaag attacacgcc gagtcgttca
     2461 ggaacatgta ctcactgcag ggattagatg cgtgaattct gtccgtgttc gggcaggtgt
     2521 gccagttgtt gatggtggtg tcgtactgga tgcccgggtc cccgcacatg tgagcgcttt
     2581 cggcaatcca ctgcatcagg tcccgcgcgc ggtaggtgtc catcacccgt ccgcccacca
     2641 cggcgtgtgt ctgccattcg ccgtcgtttt ccaccgcccg catgaactcg tcggtaacgc
     2701 gcaccgagtt gttcgcgttc tggaaatgaa ccgaatcgta cgcgccgccc ggaacgttga
     2761 accccggatc ataccccgct tcgatcagga tgcgcgcttt cttttcttcc tccactttgc
     2821 agaggatgaa ctcgcggata tccgggtgat caatgttcag gatcaccatt ttggccgcgc
     2881 gacgagtctt tccacccgac tttatggaac cggcgaacgt atcgtacccg cgcatgaagg
     2941 agaccggacc ggaggccgtt cccccgctgg aaagcgcttc gcgcgaggat cggatgggag
     3001 aaaggttcgt accggtgccg gagccccact tgaacagcat gccctctgtc ttggcgaggt
     3061 cgaggatgct ctccatttcg tcccgcacgg agttgatgaa gcaggccgat gcctgggggc
     3121 gcgcgcccgg aacgccgatg ttgaaccaga cggggctgtt gaacgacgcg cgctggttga
     3181 tgaggatggc ggtcagctcc gcccgaaagg tctcccgctc ctcttcatcc aggaagtagc
     3241 cgtccttgtc accccattcg gtgatggtgt ctaccacgcg cgtgacaagc tggcgtgcgc
     3301 tgcgttcacg ctgcggcgac cctttcggcc cgtgaaagta cttggacacg accacgttgg
     3361 tagcgagctg gctccagctt ttgggtacct ccacatcccg ctgttcgaat acggtctcgc
     3421 ccttctcgtt gccgatggtg gccgtgcgga tatcccattc gatctcgtcg aagggatgcg
     3481 ttccgggccg ggagaagtag cgcgagatgc ggaactggtg cggtgcctgc gcggtgtcgg
     3541 tggcagtcgc gtccgggttc gggctgacag acatgcaaac tctccttctg aacaaaagaa
     3601 ccgtggtaca caatatatag tgtaaagttc aggatttttg cactagcggt gggcctagag
     3661 aggatagaaa gcccggaccg cgtcgtcaag atcagcaacg ggcgcggaaa aaatgatccg
     3721 gcgatctgcc cgtgcatgcg aaaaaccgca gaaagcaata atggccgacg gggtggcggt
     3781 ttgggctgcg ccacgcgcaa gtggccacgg ggcgatatct tgggcaaaaa aaagtgaacg
     3841 ccactaccgg tagcattctg gcccgcaatt tgcggtgtgc gggagcgcgc tcaatcctgg
     3901 agctaaacag ttgttggaca gggaattacg cgacatctgc cggtttgagc agcactactc
     3961 gcccgccgaa accacggaac gcccgccagc cacggtagag ctgggcggtc agcttccagg
     4021 cacggccaac tca
//
LOCUS       DAOTNG010000286         4031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_17960_length_4031_cov_4.848592, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000286 DAOTNG010000000
VERSION     DAOTNG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17960_length_4031_cov_4.848592"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..2479
                     /locus_tag="P5179_11885"
     CDS             44..2479
                     /locus_tag="P5179_11885"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS95962.1"
                     /translation="MPAPNSSVHYDAHSFIINGKREFLTSGSIHYFRVPRALWRDRLE
                     KAKAAGLNTIQTYVAWNWHEPREAVFDFTGDHDFEHFLQLCEEFGLFVVARPGPYICA
                     EWDFGGFPAWLLTKRGLTLRHHDPVYLQYVDRWFEELIPIIARHQVSRGGCVILVQIE
                     NELGNVVPEKKDADAYMSHLRDLVRNLGIEVPLITCAGTAEGAIEAVNSHEPADQFPE
                     FRSRQPNMPIHCTEFWTAWYLTWHRDRRWVNRSPEHLAYHTWRVLAEGGSGYNYYMWY
                     GGTNFAYTTMYLQTTSYDYDAPLSEAGGIGRRGRLTIPIAHFAQAFAHILADSDASCE
                     KRADGIEVWRRSNSQGTLLFCKNPSDRPVTLPAATEDTKELGPVHVPPESVAPIVAGA
                     TIAPCSAVRLVTACPVHGIYRVGACYIIVLMGARGSSIHCTLVHGSTGRGFLHTLKGD
                     EPVVAGDWNAGERIRILVVPDAFSGRVWCAGDRLVIGASYARAVPEGVEIQHTAATVS
                     TGAWTVTESAITHVPSRAPRLPAPPALSPWDSCIENAHLPCKKPRTEPNGWVGSLRPV
                     PRDLVGDHEGYGWYRAVFHSREARTALLHIEAVSDRALVFVNGGHVSTSIEPVEDRRF
                     QPSVTVPVELKAGENVIAVLVDNLGHVKGDWQIGEVGKRGSPTMRDDLKGILGRVWVD
                     ADEVLEWEMHAKLLGEHEEWYRPGANAPWGSQHQPSPVSWYRASFTMDTSVRRPLWVR
                     VTGLNKGCIWVNGHHMGRYWNINGHTDYYVPECWLADSNTIVLFEETDATPEAVALVW
                     DDVATLVESTVLK"
     gene            2642..3382
                     /locus_tag="P5179_11890"
     CDS             2642..3382
                     /locus_tag="P5179_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS95963.1"
                     /translation="MILAAIQLDTVAMEVNANVHKALHWTRTAFENGAKYVFLHEGLT
                     ADYAPDPMKYGRALESVEVFGFAMLAKRYGGYVALGLNEVYKGAPYISCVYLDGNGVI
                     DVYRKSYLWSLPDRDNYIGYIHGFRQELGILGHGDGPRNVRVGDLLIGSLICADGNNP
                     EAWEPFRKEPPDIVFYQNNRGNVDEARNPDFCREINRPMVCTNRVGFSYYHFQLGGTR
                     FIRRDGTIAVAANTEGKEQAIYARWQDL"
     gene            3411..>4031
                     /locus_tag="P5179_11895"
     CDS             3411..>4031
                     /locus_tag="P5179_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95964.1"
                     /translation="MTHRERYIQALTFGNPDKIPFSPGGPREKTLKRWHAEGLPENVN
                     YMEALYDILGFHPEPAEKPAPGLNVDFRMIPQFEEKVLEHKNGHYIVQDWMGAITEIS
                     DEYDYTYIRAARDFVTRKWHRFPVQNRQDFEEKIKWRYDPRAPGRFPPDFEERCEKNR
                     DRDTVAAIHFNAPFWQLREWCGFEGLCTLMVDDPEFVEEMAEFWTNF"
BASE COUNT          828 a         1303 c         1150 g          750 t
ORIGIN      
        1 cccggccgcg ttcgccaacc cgtgccttcc cggagggaac tccatgccag cgccgaattc
       61 gtcagtacac tacgacgcgc acagcttcat catcaacgga aaacgtgagt ttctcacgtc
      121 cggttccatt cattatttcc gcgttccgcg cgccctctgg cgggatcggc tggagaaggc
      181 gaaagccgcg ggcctcaaca ccatccagac ctacgttgca tggaactggc acgaaccgcg
      241 ggaagcagtg ttcgatttca cgggtgatca tgattttgag cacttccttc aactctgcga
      301 ggagttcggc ctgtttgtgg tcgcgcgccc cggcccgtac atctgcgcgg aatgggattt
      361 cggcggattt cctgcgtggt tgctgacaaa acgcggtctc accctgaggc accacgaccc
      421 cgtctacctc cagtacgtcg accgctggtt cgaagaactg attcccataa tcgcgcgcca
      481 tcaggtctcc cgcggcggtt gcgtcatcct cgtccagatc gagaacgaac tggggaacgt
      541 cgtaccggaa aagaaagatg ctgatgccta catgtcccac ctgcgcgatc tcgtgcgcaa
      601 cctcggcatc gaagtcccgc tcataacatg tgccggaacc gcggaaggcg cgatcgaggc
      661 ggtcaacagc catgaacctg ccgaccagtt ccccgaattc cgttcccgtc aaccgaacat
      721 gcccatccac tgcaccgagt tctggaccgc atggtatctc acgtggcatc gcgaccgccg
      781 ctgggtgaac cgctctccgg aacacctcgc ctaccacacc tggcgggtgc tggccgaagg
      841 aggttcgggg tacaactact acatgtggta tggcggcacc aacttcgcct acaccaccat
      901 gtacctccag acaaccagct acgactacga tgctccgctc agcgaggccg gaggaatcgg
      961 caggcgtggc cggttgacca ttcccatcgc gcatttcgct caggcgttcg cgcacattct
     1021 cgccgattca gacgcctcgt gcgagaaacg cgcggacggc atcgaggtct ggcgacggtc
     1081 caactcccag ggcacgcttc ttttctgcaa aaacccgtca gaccgcccgg tcaccctgcc
     1141 tgccgccacg gaagacacga aggaactcgg cccggttcac gtgcccccgg aaagcgtcgc
     1201 acctatagtt gccggcgcta cgatagcgcc ttgttccgcc gttcgtctcg tcaccgcatg
     1261 cccggtacac gggatttacc gcgtcggcgc gtgctatatc attgtcctga tgggcgcgcg
     1321 cggctcttct atccattgta ccctcgtgca cgggtctacc gggcgcggtt tcctccacac
     1381 gctgaaagga gatgaacccg ttgtcgcagg ggattggaac gccggagagc gcatccggat
     1441 tctggtcgtt ccagacgctt tctccggccg tgtctggtgc gcgggtgaca ggctcgtgat
     1501 tggcgcgagt tacgcccgtg cggtgcccga aggcgtggaa atccagcaca ctgctgcaac
     1561 cgtctccact ggcgcgtgga cggtcaccga atccgcgatc acgcacgttc catcccgagc
     1621 tccccgtctc cccgcccctc ccgcgctcag tccatgggat agttgcatcg agaatgccca
     1681 tctcccctgt aagaaaccac gtacggaacc aaacggatgg gtgggcagcc tccggccggt
     1741 tccacgtgat ctagtcggtg accacgaagg ctacgggtgg taccgtgcgg tctttcattc
     1801 acgtgaggcg cggaccgcac tcctccacat cgaggcagtc agtgatcgtg ccctggtatt
     1861 tgtcaacggc ggacacgtga gcacctcaat cgaaccggtg gaagaccggc gcttccagcc
     1921 cagcgttacc gttccggtgg aattgaaagc cggagagaac gtgatcgcag ttctggtgga
     1981 taacctcggg catgtgaagg gcgactggca gattggggag gtgggcaaac gcggcagccc
     2041 caccatgcgc gacgacctga agggcatcct tggccgcgtc tgggtggatg cggacgaagt
     2101 gctggaatgg gaaatgcacg cgaagctttt gggcgaacat gaggaatggt accgccccgg
     2161 tgcgaacgca ccgtgggggt cgcagcatca accctcgccg gtgtcctggt accgcgcctc
     2221 gttcacgatg gatacctctg tcagacggcc tctctgggtg agagtcacgg gcctcaacaa
     2281 aggctgcatc tgggtgaacg gccaccatat gggccgctac tggaacatca acggccacac
     2341 cgattactac gtgccggagt gctggctggc ggattcaaac acgatcgttc tgtttgagga
     2401 aaccgacgcg acgcctgaag cggtcgcgct tgtgtgggat gacgtggcaa cgctggtgga
     2461 gtcaaccgtc ctcaagtagc gtccctcgca cctgcgccgc tcacgccgcg gagcttccgc
     2521 gaacgttcgg atcttctctg actgggcctg ccttgcgccg cgccccgccc ttgttcccct
     2581 cattcacgcg gactgatatt ccttgcggca cgcctgcgcg gagacatcag ggagacgcat
     2641 catgatactc gctgcgatcc agctcgatac cgttgccatg gaagtgaacg cgaatgtcca
     2701 caaagccctc cactggacac gaaccgcgtt cgaaaatggc gcgaaatacg tgtttctcca
     2761 tgaggggttg actgccgatt acgcccccga ccctatgaag tacggtcgcg cacttgaaag
     2821 cgtggaagtc ttcggcttcg ccatgctcgc gaagcggtac ggtggttatg tcgcgctcgg
     2881 gctcaacgag gtctacaaag gcgcccccta catctcatgc gtgtacctcg acggcaacgg
     2941 cgtcatagac gtctaccgca aaagctacct ctggtccctg cccgaccgcg ataactacat
     3001 cggatacatc cacgggttcc ggcaggaact cggtatcctg gggcacgggg acggcccgcg
     3061 caacgtccgg gtcggcgacc tgctcatcgg ctcccttatc tgcgcggacg ggaacaaccc
     3121 tgaagcgtgg gaaccattcc ggaaggaacc accggacatc gtcttctacc agaacaaccg
     3181 cggaaacgtt gacgaagcgc gcaaccccga tttctgccgc gaaatcaacc gcccgatggt
     3241 ctgcaccaac cgggtgggct tctcctatta tcatttccag ctcggtggaa cacgattcat
     3301 ccggcgggac ggcaccatcg ccgtcgcggc aaacaccgaa ggcaaagagc aggcaatata
     3361 cgcccgctgg caggatctct gagccaactg caaaccgaaa ggctgatcag atgacgcatc
     3421 gcgaacgcta catccaggcc ctcaccttcg gcaacccgga caaaatcccc ttcagccccg
     3481 gtggcccgcg cgaaaaaacg ctcaaacgct ggcacgcgga aggcctgccc gaaaacgtca
     3541 actatatgga ggcgctgtac gacatcctcg gtttccatcc cgaacccgcg gaaaaacccg
     3601 cccccggcct gaacgtggat ttccgcatga ttccccagtt cgaggaaaag gtgctcgaac
     3661 acaaaaacgg gcactacatc gtgcaggact ggatgggcgc catcaccgaa atctccgacg
     3721 aatacgacta cacctacatc cgcgccgcgc gcgattttgt tacgcgcaaa tggcaccggt
     3781 ttcccgtcca gaaccgccag gatttcgaag aaaaaatcaa atggcgctac gatcccaggg
     3841 ctcccgggcg cttccccccg gatttcgagg agcgatgcga aaaaaaccgt gaccgcgaca
     3901 ccgtcgccgc catccacttc aacgcgccct tctggcaact gcgcgaatgg tgtgggttcg
     3961 agggcctttg cacgctgatg gtggacgacc ccgaattcgt cgaggagatg gcggagttct
     4021 ggacaaactt c
//
LOCUS       DAOTNG010000287         4004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18102_length_4004_cov_4.932388, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000287 DAOTNG010000000
VERSION     DAOTNG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18102_length_4004_cov_4.932388"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..882)
                     /locus_tag="P5179_11900"
     CDS             complement(463..882)
                     /locus_tag="P5179_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRS95965.1"
                     /translation="MPRPRNPRQVRCIPCTDYFKPRGIPLRALEEVTLAVEELEALRL
                     ADLEGMEQVAGAEQMEVSRPTFARVLASARQKVADALVNGKALRIEGGVFRMAENVPF
                     FGRHGHGWGQGRGGRCGRRFRGGQGFDGGEEPSEQSA"
     gene            complement(1009..1374)
                     /locus_tag="P5179_11905"
     CDS             complement(1009..1374)
                     /locus_tag="P5179_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS95966.1"
                     /translation="MDVRRLVDHLDRRRMVLCHNGTIYSQALFGELTPDEVCPGVFAY
                     APGWGVLDPGMTMEAHEHPIAEFYVFVSGKGRMRLGDTWFDVEPNMSVNIPPGVEHEL
                     ANDATSPLPLVFVSVGLRL"
     gene            complement(1352..2179)
                     /locus_tag="P5179_11910"
     CDS             complement(1352..2179)
                     /locus_tag="P5179_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95967.1"
                     /translation="MNGRHKTTLWREHVALWRDAWRRVVAHRRQVVLITLMLWLPWRF
                     VVPLVDRRLGFSAFAEFPLEWIADALYDPLYGATILAVLAEMYPISVRSALLRGFWLV
                     PRLWLIEIKVAIWGLAVPYLILHLGAAIVLTLWPSYGAARALFAVTFVSMAWVLVVVV
                     RYMLAAAVMVAETQGHTWNEEQAPSTGWALFAARQLIRGNVGQAVLVMAVGQGVISLV
                     VSLLPSGDTVLAQFLNATGMVVTALWWALLWSFCQFCLNRARGVTAQEATYGRQKAR"
     gene            complement(2176..2775)
                     /locus_tag="P5179_11915"
     CDS             complement(2176..2775)
                     /locus_tag="P5179_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRS95968.1"
                     /translation="MLPTEEAGAPQPARLTRPVVLIGFMGAGKTKVGRIAAQYIGCDF
                     VDTDHIVERSAGKSIPQLFAEIGEERFRHLETEAIEHALNTHGGIIALGGGAATREEN
                     WQLLRRSGAMTIYLQASPRTILARVADKGTRPLLAGLDRQQMLEKITSLLEQREPWYL
                     RADITLPTDNSLDKYGMADTLVARLKEAGISAAESHRTS"
     gene            complement(2712..2909)
                     /locus_tag="P5179_11920"
     CDS             complement(2712..2909)
                     /locus_tag="P5179_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95969.1"
                     /translation="MGKRIPKPEWFVCETCGKQTRALRRDVLDEHYNALGKTPLWNCE
                     ECYQQKRRERLSQPGSPDPSS"
     gene            complement(2902..3864)
                     /locus_tag="P5179_11925"
     CDS             complement(2902..3864)
                     /locus_tag="P5179_11925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS95970.1"
                     /translation="MVAKGRVTVEPLSGQFSRAAGALIRTVLQEEFGAAIRACEDADL
                     EDVLSAYAGPESAAWVAHRDGVVCGVAAVRETESGALELKRFYVRAECRGKGIGGTLL
                     REVERRAAEDGYQQIVLTTTHLMARAQEVYRHFGYQLDSTERKGAVTLHTYRKRLVRA
                     ALGARRLSGPLPNTGTGEIAVVVERPRGSCEGWHWDRQTHTLVLTEYYDRPVPVNYGL
                     APEWVNPADQDALDVILLDDRRMQPGETLVAVPAGVLLRPDGDDKLLAVPAGGAVRPV
                     GLDERSRARVSAWWDDAHQPVGWEGEEGIDRLFARCRRQGAMNG"
BASE COUNT          752 a         1425 c         1090 g          737 t
ORIGIN      
        1 cgctcgtttc taccgggatt gcgatcttca tgaagtcctt cctttcctgt gaatcgtttc
       61 gaaccagaac gctcccggga tatcaaaccc tccagcgtcc gccccttcgg taaccaccgg
      121 gcgctccccg ccgcgcacac gccgaacgga tccatgcccc gcatctcgca aatccccggg
      181 aaaacggatt cgcagcggga gtaaaaacag gcgacggtga actcgcaacc ggggcttccg
      241 acggccggac atcttcctcg cgcgcttccc atttcgttat cgccccgtgc tgacacgctt
      301 ccgcgcacag gccgcactcg gtgcacaaca cgaaattgat cttcggtttg tcctctcgga
      361 tttccagtgc acccggtgtt ttgcaggcgt tcacgcagga aaagcaccgt gtgcaaagat
      421 cataatttat tgtatacatt cgctgttacc tcatgttcga aatcaggcac tctgttcaga
      481 gggctcctca cccccgtcaa acccttgccc tccgcggaat cgccgcccgc accgtccgcc
      541 gcggccctgt ccccatccgt gtccgtgccg cccgaagaaa gggacattct ccgccatccg
      601 gaacacgccg ccttcaatcc ggagcgcttt cccgttcacc agcgcatccg ccaccttctg
      661 ccgggccgag gcgagcaccc gtgcaaatgt ggggcgcgat acctccatct gctctgctcc
      721 cgccacctgc tccatgcctt cgagatctgc cagccgcaac gcttccagtt cctccaccgc
      781 cagggttacc tcttccagcg cccgcaacgg gatgccccgc ggtttgaagt aatccgtgca
      841 cggtatgcat cgcacctggc gtggattccg tggtctcggc atagggtgtc gcctcctgag
      901 ttatgagcat atgttcataa tgaggataca cgacgaaggc tgacttgtca agagacaaag
      961 cggggaatca ggaacgcgga tttgttctgg caggggccgg cgcgaccgtc acagcctcag
     1021 gccgaccgaa acaaacacga gtgggagagg cgatgtggcg tcattcgcga gctcatgttc
     1081 caccccgggc gggatattca cggacatatt cggctcaaca tcgaaccacg tgtcgcccag
     1141 ccgcatccgt ccctttccgc tcacgaacac gtaaaactca gcgatcgggt gctcgtgcgc
     1201 ctccatggtc atccccgggt ccagcactcc ccatcccggc gcataggcga acacgcccgg
     1261 gcacacctca tccggggtga gctcaccgaa cagagcctgc gaatagatcg tcccgttgtg
     1321 gcacagcacc atgcggcggc ggtcgagatg atcaacgagc cttctgacgt ccatatgttg
     1381 cctcctgtgc ggtcaccccg cgcgccctgt tgagacagaa ctggcaaaag ctccacaaaa
     1441 gcgcccacca gagcgcggta accaccattc ctgtcgcgtt cagaaactgc gcgagcaccg
     1501 tatcgcccga aggaaggagg gacacgacaa gcgagataac cccttgcccc accgccatca
     1561 cgagaaccgc ttgccctacg ttcccccgga tcaactgccg cgccgcgaaa agcgcccacc
     1621 cggtggaagg cgcctgctcc tcgttccagg tgtgcccctg cgtttccgca accatcaccg
     1681 cggctgccag catgtaccga accaccacca ccagcaccca cgccatgctg acaaatgtca
     1741 ccgcaaaaag cgcgcgggcg gcaccgtaac tcggccacag cgtcaacacg atcgccgccc
     1801 cgagatggag gatcaggtag ggcaccgcca gcccccagat cgctaccttg atctcaatca
     1861 gccacagacg aggaacaagc cagaaccccc tcaggagagc gctccgaacc gaaatcggat
     1921 acatttccgc cagcaccgca aggatggtgg ctccgtacaa cggatcatac agcgcgtccg
     1981 caatccattc gagcggaaac tcggcgaacg cactgaagcc gagccggcgg tccaccagcg
     2041 gaaccacaaa ccgccacggc agccacagca tcagagtgat caacacgacc tgccgacggt
     2101 gtgcaaccac ccgccgccag gcgtcccgcc agagcgcaac atgttcccgc cacagcgtgg
     2161 ttttgtgccg tccgttcatg acgtccggtg cgattccgcc gcgctgatgc cggcttcttt
     2221 gaggcgcgcg accagtgtat ccgccatgcc gtacttgtca agcgagttgt cagtcgggag
     2281 ggtaatgtct gcccggaggt accacggttc gcgctgttcc agcagggacg tgatcttttc
     2341 cagcatctgc tggcgatcga ggccggccag aagcggtcgc gtacccttgt ccgcaacccg
     2401 tgcgagtatt gttctcggcg aggcctgcag gtagatcgtc atggctcctg acctgcgcag
     2461 caactgccag ttctcctcac gcgtggcagc tccaccgccc agtgcgatga tgccgccgtg
     2521 cgtgttcagc gcgtgctcga tggcttccgt ttcaaggtgc cggaacctct cttcgccgat
     2581 ttcggcaaaa agctgcggga ttgacttgcc ggcggagcgc tccacgatgt ggtcggtgtc
     2641 cacgaaatcg cacccgatat actgcgcggc gattctgccc accttcgtct tgccggcgcc
     2701 catgaagccg atcaggacga cgggtcgggt gagccgggct ggctgaggcg ctcccgcctc
     2761 ttctgttggt agcattcttc gcagttccag agaggagtct tcccgagggc attgtagtgc
     2821 tcgtccagca catcacggcg caatgcccgc gtctgcttgc cgcaggtttc gcacacgaac
     2881 cattcgggct tcggtattcg cttacccatt cattgctcct tgccgccgac atcgcgcaaa
     2941 aaggcggtcg atgccttcct caccttccca tccgacaggc tgatgggcgt catcccacca
     3001 cgcagaaaca cgtgcccgcg accgttcatc aaggccaacc ggccgcaccg caccccccgc
     3061 cggaaccgcc agcagcttgt cgtcgccgtc cggacggaga agcactcctg ccggcacggc
     3121 gacgagcgtt tcccccggtt gcatccggcg gtcgtccagc agaatgacgt ccagcgcatc
     3181 ctgatccgcg gggttcaccc actccggggc caggccgtag ttcacgggga ccggtctgtc
     3241 gtaatactcc gttaatacca gggtgtgtgt ctgccggtcc cagtgccacc cctcacacga
     3301 tccgcggggc cgttccacca ccaccgcaat ctcacccgtg cccgtgtttg gcaaaggtcc
     3361 ggacagtctt cgtgccccga gtgctgcccg cacaagccgc tttcggtacg tgtgaagcgt
     3421 caccgcgccc ttgcgttccg tactatccag ttggtacccg aagtgccggt acacctcctg
     3481 cgcccgcgcc ataaggtgtg tcgtcgtaag cacgatctgc tggtacccgt cctctgccgc
     3541 gcggcgttcc acctctcgca ggagtgtgcc gccaatgccc ttcccgcggc actcagcgcg
     3601 cacgtagaat cgcttcagtt ccagcgcgcc gctctcggtc tcgcgcacgg cagccacgcc
     3661 gcacaccacg ccgtcgcggt gcgccaccca cgcggcgctc tccggacctg cgtacgcgga
     3721 gagaacgtct tccagatctg catcttcgca cgcccggatc gcggcgccga actcctcctg
     3781 aagcaccgtc cggataagcg ctcccgcggc gcgggaaaac tgccccgaaa gcggctcaac
     3841 cgtcacccgc ccctttgcta ccatcggtgc cagcggtact ccggcttcca ccacaggtcc
     3901 tgccgcgcga tcacctgttc cgtgccgacg acgtacacct ccggtcttcc ggttttctgc
     3961 gagccaaaca ggatgtactt cccgtcgggg ctgaacgaag gatg
//
LOCUS       DAOTNG010000288         3974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18267_length_3974_cov_4.271243, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000288 DAOTNG010000000
VERSION     DAOTNG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18267_length_3974_cov_4.271243"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1578
                     /locus_tag="P5179_11930"
     CDS             241..1578
                     /locus_tag="P5179_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS95971.1"
                     /translation="MGEPKRPKRPRGRARLLEGKCIACGALCQAECPADAIEMNDRGE
                     PIIILENCIGCKKCVKVCPASALEMFFTPEEEAVLAELARLGFAKEAPEKEGPVPKVD
                     QSYRGVWVFVEQQNGRAAAVSWELLGAGAKLAADLEVDLCAYVLGHEIGHLADEAFAY
                     GAKKVYLVDDPILKNYRTEPYLKASSDLIRKYKPEVVLMGATGLGRDLAGAVATDLHT
                     GLTADCTGLAIDKAGRLLEQTRPAFGGNIMATILTERHRPQMATVRPHVMPKPEPVSG
                     AHGAIVVEPLDMSEDDIRVKVLEVREDEGAANRVDIAGAEVLVSGGRGMQGPENYRLL
                     QELADVLGGTVASSRAAVDAGWMPYERQVGQTGKTVRPKIYIACGISGAIQHLVGMQD
                     ADLIIAINRDKDAPIFEVAHFGLVGDLFQIVPLLTKRFREMAGKPSDSGNQKG"
     gene            1584..3584
                     /locus_tag="P5179_11935"
     CDS             1584..3584
                     /locus_tag="P5179_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS95972.1"
                     /translation="MSQTENVIFAVVLAIAVAAFLQSVWRLVSFIRLGKPDGRLSGEL
                     VRRFWTMLVYAFWQRRVRKEPYGWNHFFLFWGFIILFLANGEFVIAGLFPSLTFELLG
                     ERIYGVLLFLFDVVSMIVLVNVAIAFFRRLVLKPAHIEYNSRDGYLILSLIGALMLAF
                     FCLYGSEFASGTMSVAGVMPFTETIFAPLMGSLFGSAAPIAARVFWWLHGLIVLSFLN
                     YLPYSKHMHILTAIPNCYARSFEYPATVPREEFKPGAQFGVSVVDEFSWKDLLDFTAC
                     TECGRCNQNCPATSTGKPLNPRLLVHDGKVNLLTNGPKLLQGNRSANLLPLIGTTDEH
                     EGTVGEDALWACTTCGACMTNCPVFIEHVPKIIKMRRHLVENEARFPHELSVFFESVE
                     QRSNPWGITPGDRAKWARDLSVPLMSDAKCDTLFFVGCAGAFDARNKKVATAVVRALK
                     AAGVEFAILGTEEKCCGDSVRRLGNEYLFERMANANVELFKKYDVRRIITYCPHCYTT
                     LKHDYRQFGMETQVVHHSEFLHELLETGRLKLDGNKPAVDGKVLYHDSCYLGRYNGLY
                     EEPREVVQRVTGRAPLEMDRRLEKSFCCGAGGGRMWMEEEPEHRINANRVREALGKDA
                     DVIAVACPYCMTMFDDGIKDEHAEGRVKVMDIAELVAARLPG"
     gene            complement(3725..>3974)
                     /locus_tag="P5179_11940"
     CDS             complement(3725..>3974)
                     /locus_tag="P5179_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95973.1"
                     /translation="HDTPLGGSRVTCAPRTSSGKGASRGVWLGSLSQAWQGYKVEEAV
                     NEALVLFRPVLVLFCHVSDYSRPDPTIALIERRRMNSA"
BASE COUNT          792 a         1092 c         1342 g          748 t
ORIGIN      
        1 tcagcctctc gcgggtgggg ttgaaaggct cgccgacgca cgtgaagaag atttttgcgc
       61 cggagcgcgc ccgtggtgaa atactggggg atgcggaaaa cgatccggaa ggcgcggtcg
      121 ccctcctgat aagacgactg attgaaaagg acctgatttc cgtcgggcga taggccgcag
      181 gatgttgctg ccggccaacc cgtaacaagg agagattccg cggtgcccga gaaaagcagt
      241 ctgggtgaac ccaaacgtcc gaaacggccc agaggacgcg cgcgtcttct ggagggcaaa
      301 tgcatcgcgt gcggcgcgct ctgccaggcg gaatgcccgg cagacgcgat cgagatgaac
      361 gaccggggag aacccatcat catcctcgag aactgcatcg gctgcaaaaa gtgcgtgaaa
      421 gtgtgcccgg cttccgccct ggagatgttc ttcacgccgg aggaggaggc ggttcttgcc
      481 gagcttgcgc ggctcggttt cgcgaaagaa gcgcccgaga aggaggggcc ggttcccaaa
      541 gtggaccaga gctaccgtgg cgtgtgggtg ttcgtggagc agcagaacgg acgcgcggcg
      601 gcggtaagct gggaactgct cggcgccggc gccaaactcg ccgccgacct cgaggtggac
      661 ctctgcgcgt atgtgctggg gcatgaaatc ggccatctcg ccgacgaggc gttcgcatac
      721 ggcgcgaaga aggtctacct cgttgacgat ccgattctga agaactaccg tacggaaccc
      781 tacctcaaag cgtcgtccga tctcatacgc aaatacaagc cggaagtggt actgatgggc
      841 gccacagggc tcggaaggga cctcgccgga gcagtcgcca ccgatctgca caccggcctg
      901 acggcggact gcaccgggct cgccatcgac aaggctggca ggcttctgga acagacgcgg
      961 cctgcgttcg gcggcaacat catggccacc attctgacgg agcggcaccg cccccagatg
     1021 gcaacggttc gcccgcacgt catgccgaaa ccggaaccgg tgagcggcgc gcacggcgca
     1081 attgtcgtgg aacctctgga catgtccgag gacgacattc gcgtcaaagt gctcgaagtg
     1141 cgggaagatg agggcgccgc caatcgtgtt gacatcgcgg gcgcggaggt tctggtctcc
     1201 ggcggccgcg gaatgcaggg gccggagaat taccgcctgc ttcaggaact ggcggacgtt
     1261 ctgggcggaa cggtggcctc ctcccgtgcc gccgtcgacg cgggctggat gccctacgag
     1321 cgacaggtcg ggcaaacggg aaaaacggtg cggccgaaga tatacatcgc gtgcggcatc
     1381 tccggcgcga tccagcacct ggtgggcatg caggatgctg acctgatcat cgcgatcaac
     1441 cgggataagg acgccccgat tttcgaagtg gcgcacttcg gcctggtcgg tgatctcttc
     1501 cagatcgtgc ctcttcttac gaagaggttc cgcgaaatgg cgggcaaacc gtccgactcc
     1561 ggcaaccaga aaggctgacg agcatgtctc agacagagaa tgtgatcttt gcggttgttc
     1621 tggcaatagc agtagccgca tttctgcagt cggtctggcg gctggtgtcg ttcatccggc
     1681 tggggaaacc ggacggcagg ttatcgggag aactggtccg gcgtttctgg accatgctgg
     1741 tgtacgcgtt ctggcaacgg cgggtgagga aggaaccgta cggctggaac cactttttcc
     1801 tgttctgggg gttcatcatc ctcttcctgg caaacgggga gttcgtgatt gccgggcttt
     1861 tcccgtcact aacctttgaa ctgctcgggg aacgtatcta cggggtgctc cttttcctgt
     1921 ttgacgtcgt cagcatgatt gtgctggtca acgttgccat cgcgttcttc cggcggctgg
     1981 tgctgaaacc ggcgcacatc gagtataaca gccgggacgg gtatctcatt ctgagcctca
     2041 tcggggcgct gatgctggcg tttttctgcc tgtatggctc ggaattcgcg tccgggacga
     2101 tgagtgtggc gggcgtcatg ccgttcacgg agacgatctt cgcgccgctg atgggcagcc
     2161 tgttcggcag tgcggcgccg attgccgcgc gcgtattctg gtggctgcac gggctgatcg
     2221 ttctgagctt tttgaactac ctgccgtaca gcaagcacat gcacatcctc accgcgatcc
     2281 cgaactgcta cgcgcggtcg tttgaatatc cggcgacggt gccgcgcgag gagttcaaac
     2341 cgggcgcgca gtttggcgtg tcggtggtgg acgagttctc gtggaaagac ttgcttgact
     2401 tcaccgcatg caccgaatgc ggacgctgca accagaactg cccggcgacc agcaccggga
     2461 agcctctcaa cccgcgcctt ctggtgcacg acgggaaggt gaatctgctg acaaacgggc
     2521 cgaagcttct gcaggggaac cgctcggcga atctgctgcc cttgatcggc acaacagacg
     2581 aacacgaggg gactgtgggc gaggacgctc tgtgggcgtg caccacgtgc ggcgcctgta
     2641 tgacgaactg cccggtgttc atcgagcatg ttcccaagat catcaagatg cgccggcatc
     2701 tggtggagaa tgaggcccgc ttcccgcacg aattgagtgt ctttttcgag tccgtcgagc
     2761 agcgatcgaa tccttggggc atcacgccgg gagaccgcgc gaaatgggcg cgtgatctgt
     2821 cggtgccgct gatgtcggac gcaaaatgcg acacgctgtt tttcgtggga tgcgccgggg
     2881 cgttcgacgc gcggaacaag aaggtggcga ctgcggtggt gcgcgcgctt aaggcggcag
     2941 gcgttgagtt tgcgatcctc gggacggagg aaaaatgctg cggggattcc gtccgccggc
     3001 ttggcaacga gtatctgttt gaacggatgg cgaacgcgaa cgtcgagctg ttcaagaaat
     3061 acgacgtaag aagaatcata acctattgcc cgcattgcta caccacactg aagcacgact
     3121 accggcagtt cgggatggag acgcaggttg tgcaccactc cgaattcctg catgaactgc
     3181 ttgagacggg gcgcctgaag ctggacggga acaaaccggc cgtggacggg aaggttctgt
     3241 accacgattc gtgctacctc ggacggtaca acgggttgta cgaggagccg agggaagtgg
     3301 tgcagcgggt gacggggcgg gcaccgctgg agatggaccg ccggctagaa aagagcttct
     3361 gctgcggcgc gggcgggggg aggatgtgga tggaagagga gccggaacac cgcatcaacg
     3421 cgaaccgggt gcgggaagcg ctggggaaag acgcggatgt gattgccgtg gcgtgcccct
     3481 attgcatgac gatgttcgac gacggcataa aagatgaaca cgcggagggg cgtgtgaagg
     3541 tgatggatat tgcggagctg gtggcggcgc ggctgccggg gtgatgaggg gggcgtgcgg
     3601 tgcctctccc cgatgagtta cgagcttttc gtgcgtctgc cacgactgca gcgtcttttc
     3661 ctttggagtt cctgagtttg atcgcggcga gcggcagctt ctgtgactga ttcgatgcag
     3721 ctcgtcaggc ggagttcatt cgccggcgct caatcagggc aatcgtggga tcgggacggg
     3781 aataatcaga aacatgacag aataagacaa gaacaggacg gaataagaca agtgcttcat
     3841 ttacagcctc ttccaccttg tacccctgcc atgcctggga caaactgcca agccagacgc
     3901 cccgcgacgc gcccttgccg gaagaggtac gtggtgcaca ggttacccga gaaccgccga
     3961 gcggcgtgtc gtgt
//
LOCUS       DAOTNG010000289         3934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18475_length_3934_cov_5.567414, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000289 DAOTNG010000000
VERSION     DAOTNG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18475_length_3934_cov_5.567414"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>382)
                     /locus_tag="P5179_11945"
     CDS             complement(<1..>382)
                     /locus_tag="P5179_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonide lyase"
                     /protein_id="HRS95974.1"
                     /translation="EAGRVWKIRPRRVEGEAVGHEHWLADGVHVAYHGSLPDGNKCMG
                     VIRFDNQNCVEYAFPYETGHIHSNDFRLVVGDAGDVVRIWRLEDQGFGPPRVLCRHRC
                     SSHIQQLHVHPRFNRAGTQVLFTSD"
     assembly_gap    383..392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<393..1082)
                     /locus_tag="P5179_11950"
     CDS             complement(<393..1082)
                     /locus_tag="P5179_11950"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HRS95975.1"
                     /translation="MPVGSVFQSELREFTDALSGTRIHQLTNYRGHSHHLYFTNPGWY
                     DNGRKLLISSDRMNRTNLFGIDLETGLLTQLTDYDPLPPPHEVEFLRCCVNPVRDEAY
                     YFYGRDVVALDLITGAQRTIWSLAAGFVPSQINCTADGARVVLGIHEDLSARMRVDYL
                     RGYVGFRETWAAKPLSRIIAIATDGSRSDTVWEERYWIGHTNASPTQSNLITFCHEGP
                     WELVDCRIWGLD"
     gene            1257..2327
                     /gene="hydE"
                     /locus_tag="P5179_11955"
     CDS             1257..2327
                     /gene="hydE"
                     /locus_tag="P5179_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRS95976.1"
                     /translation="MSGVPRLTRGDILRWLKENDRVRLAQLWEWADAVRHEFVGDEVH
                     LRGLIEFSNYCSRQCAYCGLNARNADVVRYRMSADEILACAHQAVGFGYGTVVLQSGE
                     DYGVTREWLGEVVRRIKAETPLAVTLSVGERTEAELAYWKDCGADRYLLRFETSNRAL
                     YDRIHPPRAGIHSDRPALLRYLRSIGYEIGSGVMIGIPGQTYEDLANDIELFRELDLD
                     MIGVGPFLPHEKTPLGKEEEQLWAPLGEQVPNSELMTYKVVALTRLVCPYANIPSTTA
                     LATVNTKNGRELGLKRGANIVMPNVTPPQYREKYEIYPAKACIRETADMCHQCMKHRI
                     ESIGRQIGKGRGDSPNRTTRRD"
     gene            complement(2346..2423)
                     /locus_tag="P5179_11960"
     tRNA            complement(2346..2423)
                     /locus_tag="P5179_11960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Pro,seq:ggg)
     gene            complement(2450..2527)
                     /locus_tag="P5179_11965"
     tRNA            complement(2450..2527)
                     /locus_tag="P5179_11965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2490..2492),aa:Arg,seq:ccg)
     gene            complement(2662..2970)
                     /locus_tag="P5179_11970"
     CDS             complement(2662..2970)
                     /locus_tag="P5179_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS95977.1"
                     /translation="MSDQSNLTKAELAQTIADQVGLTATATQAVIDGFLAAVVLALEE
                     HRSVQLRGFGTFTIIKRAPRVGRNPRTKERVPVPERAAPVFRPCRELRQAVASHKKEG
                     "
     gene            complement(2980..3864)
                     /gene="sppA"
                     /locus_tag="P5179_11975"
     CDS             complement(2980..3864)
                     /gene="sppA"
                     /locus_tag="P5179_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRS95978.1"
                     /translation="MPALTGKSRRDQIIDGIVVGLIALFVLMILAVAAGSFSARKLVP
                     GERIGLVELTGAITDPEPVNRQLRHFRDVEKVPAILLRIDSPGGYVGASQEIYRQVEL
                     IRQRGIKVIASMGNVAASGAYYVAIAADTIMANPGTITGSIGVIAEFPQADTLLRKVG
                     LAFNIVKSGRYKDTGSPTRPMRGDEQELLQSVVDDTYLQFREAVGKARNIPQARLDSL
                     TQGQVFTGRMAKAFGLVDTLGNYDDALRLAKRMAGLSDGAKVIRAPSAEKSLFQRLLG
                     NVNDLWHASSWSVSYRLP"
BASE COUNT          818 a         1138 c         1175 g          793 t
ORIGIN      
        1 gtcgcttgtg aaaagcacct gtgtgcccgc gcggttgaac ctcgggtgca cgtggagctg
       61 ctggatgtgg ctgctgcaac ggtggcggca tagaaccctc ggcgggccga atccctggtc
      121 ctcgagccgc cagatgcgaa ccacgtcgcc ggcatctccg acgaccaacc ggaagtcgtt
      181 ggagtgaatg tggcctgtct cgtacgggaa ggcgtactcg acgcagttct gattatcgaa
      241 ccgaatcacg cccatacact tgttgccgtc ggggagagaa ccgtggtaag caacatgcac
      301 gccgtcggca agccagtgtt cgtgccccac tgcttcgcct tccaccctgc gggggcgtat
      361 cttccacacg cggcccgctt cgnnnnnnnn nngtccaagc cccatatccg gcagtctaca
      421 agctcccagg ggccttcgtg gcagaacgtg atgaggttgg actgggtggg cgatgcgttg
      481 gtatgcccga tccagtagcg ttcctcccac acggtgtcag atcgggaacc gtcggttgcg
      541 atggcgatga ttctgctcaa cggtttggcc gcccaggtct ctctgaagcc gacgtagccc
      601 cgaaggtaat cgacccgcat tcgagcggaa agatcctcat ggattcccag tacgactcgt
      661 gctccgtccg cagtgcagtt tatctgtgac ggaacaaagc ccgccgcaag tgaccagatt
      721 gtccgttgtg cacccgtgat gaggtcgagg gctaccacgt cccggccgta gaaatagtag
      781 gcttcgtctc ggacggggtt cacgcagcaa cggaggaact caacctcatg gggtggcggc
      841 aaagggtcgt agtcggtcag ttgtgtgaga agaccggttt ccaggtctat gccgaaaagg
      901 ttggtccgat tcatgcggtc ggagctgatg aggagcttcc ttccgttgtc gtaccatccg
      961 ggattcgtga agtagaggtg gtgactgtga ccgcggtagt ttgtgagctg gtgaatgcgc
     1021 gtgccggaca gagcatcagt gaattcgcgc aattcagact ggaataccga acctacaggc
     1081 attcttctcc ttttctatcc tggggaaaca ctctggtcga aagaggtgca atcaaaacat
     1141 aacgggcgcc ctgaaacgga caaatgcccg cggaaagggc tgcggggaac caaaactgca
     1201 agaaggatgt attgttcgtg tgagaagcag ggagacatga tgaacaggag aggaccatga
     1261 gtggagtgcc gaggttgact cggggcgata ttcttcgctg gctgaaagaa aacgatcgtg
     1321 tgcggcttgc gcagctctgg gaatgggccg atgcggtgcg gcatgagttt gtcggagatg
     1381 aggttcacct gcgaggattg atcgagtttt ccaactactg ctcccgccag tgcgcatatt
     1441 gcgggctgaa cgcgcggaac gccgacgtcg tccgataccg catgagtgcg gatgaaatcc
     1501 ttgcgtgtgc ccaccaggca gtcgggttcg ggtacggaac cgtggttctc cagtcaggcg
     1561 aagattacgg agttaccagg gaatggctgg gagaagtagt gcgtcggata aaagcggaga
     1621 cgccgctggc cgtaactctg agtgtagggg agcgaacgga agcagaactg gcatattgga
     1681 aagattgcgg cgcggaccgc tatctcctgc gatttgaaac gtccaaccgt gcgttgtacg
     1741 accgtatcca tcctccgcgc gcgggcatcc attccgaccg tcccgccttg ctccggtacc
     1801 tgcggagcat cggctacgaa atcgggagcg gcgtgatgat aggtattccc ggccagacat
     1861 atgaggacct tgccaacgac atcgagctgt tccgggaact ggacctggac atgattggcg
     1921 tcgggccgtt tctgccccac gagaaaactc cgctcggcaa ggaggaagag caactctggg
     1981 cgcctcttgg agagcaggtt cccaacagcg agttgatgac gtacaaggtg gttgccctta
     2041 cccggctggt gtgtccgtat gcgaacattc cgagcactac cgcgttggcg acggtgaaca
     2101 cgaagaacgg gcgggaactc ggtctcaaac gaggggccaa catcgtgatg ccgaatgtca
     2161 cgccgccgca atatcgagag aaatacgaga tctacccggc caaagcctgc atccgcgaaa
     2221 cggcagacat gtgtcaccag tgcatgaaac accggatcga atcgataggc agacagatag
     2281 gcaaagggcg gggcgattca cccaacagga caacccgcag ggattgaatt ccaaaggggg
     2341 gaaaatggtc ggggcgcccg gattcgaacc ggggaccacc agacccccag tctggtgcgc
     2401 taaccggact gcgctacgcc ccgaacatca aaccagtgaa caagggggat ggtgagctag
     2461 gcaggattcg aacctgcgac ctttggttcc ggagaccaac gctctatcca ggctgagcta
     2521 ctagccctcc cgtctcacta ccgcaacttc ttcttatgac ggttcttccg aagccgtttt
     2581 ttccgcttgt gcgtggcgat cttatgccgc tttcgtttct ttccgcaagg caatggcatc
     2641 tctcctgaga acgtggtgtg ttcagccttc cttcttgtgg cttgctactg cctgccgtaa
     2701 ttcccggcag ggccggaaaa ccggtgctgc tcgttcggga acgggaaccc gttccttcgt
     2761 tcgcgggttt ctcccgactc ggggcgcccg cttgataatg gtaaatgtac cgaatccgcg
     2821 caactgaacg ctgcggtgct cctccagcgc cagcacgacc gccgcaagaa acccatcgat
     2881 gacagcctgg gtcgcggttg ccgtcagccc cacctgatcc gcgatggtct gcgccagttc
     2941 agccttcgta aggttgctct gatcgctcat atacgcctcc tatggcaaac gataggagac
     3001 cgaccatgag gacgcatgcc acaggtcgtt cacgtttcca agcagccttt gaaacaggct
     3061 cttctctgct gacggtgccc tgataacctt cgctccgtcg ctcaggcctg ccatccgttt
     3121 cgcaaggcgc agggcgtcgt cgtaattccc cagcgtgtct accaggccga acgccttcgc
     3181 catgcggccc gtgaaaacct gtccctgcgt cagcgagtcc aaccgcgcct gcgggatgtt
     3241 tctcgctttt cccaccgctt cccggaactg cagatacgta tcgtcaacca cggactggag
     3301 caattcctgt tcatcgcccc gcatcgggcg ggtgggcgaa cctgtatcct tgtagcggcc
     3361 gctcttgacg atgttgaacg ccagccccac cttgcgcaga agagtatccg cctgcggaaa
     3421 ctctgcgatc acgccgatgg acccggtgat ggtgccgggg ttcgccatga tggtatccgc
     3481 ggcgatggcg acgtaatatg ccccgcttgc cgccacgttg cccatcgagg cgattacttt
     3541 gatcccgcgc tgccggatga gctcgacctg ccggtatatt tcctgcgacg ccccgacata
     3601 cccgccaggg ctgtcaatgc gcaacagaat cgccggaacc ttctcgacat cgcgaaaatg
     3661 ccgcaactgc cgattgaccg gctcaggatc cgtgatggcg cctgttaact cgacaaggcc
     3721 aatccgttcg ccgggcacca gtttccgggc gctgaagctg ccggcggcca cggcgagaat
     3781 catcagcacg aatagcgcga tgaggcccac cacgatgccg tcaatgatct gatcacgacg
     3841 actttttccg gtcaatgcgg gcatcaggaa gcctcacggg tgttcggcaa atgagattta
     3901 caaatatacc cccgcatggg gcaaccgtca acgc
//
LOCUS       DAOTNG010000290         3902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18661_length_3902_cov_4.868209, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000290 DAOTNG010000000
VERSION     DAOTNG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18661_length_3902_cov_4.868209"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..629)
                     /locus_tag="P5179_11980"
     CDS             complement(282..629)
                     /locus_tag="P5179_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95979.1"
                     /translation="MAKVPMSAFPPNWVVTPGELVQDHLNGAGITRSSFVEKTGLAAE
                     TLDAVIADEIRITEDIAEALASALGLPVSFWVNLQAHFDSEAKRPKIPLKYVSAGQSH
                     RNTGAAAGTRVPA"
     gene            complement(622..1119)
                     /locus_tag="P5179_11985"
     CDS             complement(622..1119)
                     /locus_tag="P5179_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95980.1"
                     /translation="MIRTLREYADGPDLGVGRPLRQKTELLGVSRAQIWFPETGEVNS
                     ALREKRVEISYANKRVAKHFADRRELERQYGKPCAVAIMRRITDLQAAPNLAALSFSE
                     DPSARCHELKHDLKGYLAMDVSKSRRLLFTPGNEPIPRKSDGGLDWVRVTCVVIHRVE
                     DYHNG"
     gene            complement(1223..1855)
                     /gene="deoC"
                     /locus_tag="P5179_11990"
     CDS             complement(1223..1855)
                     /gene="deoC"
                     /locus_tag="P5179_11990"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRS95981.1"
                     /translation="MIDHTLLRPDATPEDVDRLCDEAIRHHFAAVCVHPRFAAQAVER
                     VRMTQVAVAAVVGFPHGATLPETKRAEAEALVARGVHELDMVLPVGILKANLWSEVAA
                     DIDGVVAAARQRSGTPALVKVIIEAPLLTDREKAIACIIAEQCGADFVKTCTGFSGAA
                     TVEDVALMRRVVGDRLGVKAAGGIRTREQALAFVRAGATRIGTSRGPALV"
     gene            complement(1975..2703)
                     /locus_tag="P5179_11995"
     CDS             complement(1975..2703)
                     /locus_tag="P5179_11995"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRS95982.1"
                     /translation="MNTDELERLRAEVKAALNRREAVAGASAPRDCPRLVTEECVLAA
                     RARGEAVIRVAPGAIITPLARDALARFGIAVASALGSRGAQGGKSAPDAEPVRAARTT
                     RVAVGVVATSAVEPLLVEALRAAGQAVVRVPGSARKGALLAKRVAGAVASGQADWGVV
                     VDETGLVASAVANRVKGVRAACCNDVAAAKAARERMAANVLCMGADIVAPALMREVLT
                     IWLTTEPQISPEIAAILDGMGGEA"
     gene            complement(2710..3702)
                     /locus_tag="P5179_12000"
     CDS             complement(2710..3702)
                     /locus_tag="P5179_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS95983.1"
                     /translation="MPVRLGFIGVGGIAGAHLDAAKKHGIPVTAMYDINPDTMKERQQ
                     RYSIPHIVSSAQAVADHPEVDAVVVCTPQFVHLEGIRAACNARKPLFTEKPLSRTLAD
                     SYEAVRLVEETGIVAQIGFVRRYCPEWGKFKELIEAGVIGRPVVWWMAGGGPGPGSSF
                     FNQADQGGGPMLDGMVHNYDFLRYMWGEPTRVVGSMVRLHPVNTALDTGTAIIEFASG
                     DRHTVLNSWGMPSGVRSGGLHNVLGPKGILYFSDPDNDPPVGLNRDEFGYLVTKHEGG
                     RREVHPFKKWNMYEAQLADFVKAAEEGRKQTRATITDGLRAQQIALAILGEYSF"
BASE COUNT          687 a         1387 c         1132 g          696 t
ORIGIN      
        1 gtctcgtcct gccgcgccgc gggcccggaa tacgtccacc cgaccgggat accgtccgcc
       61 gcatcctcaa atccgccgtt ggaaagcaag ttggtcatgg aagcgcctcc gtgggcagac
      121 tggggaatga ggaaggggag cacgaaaaag gatacgcacc tgaggagagg gcccatagcc
      181 ggtttctcca ccaactggcg gcgttgcccg gtcgccagcg aacaaaaacc cccggctcct
      241 ctcgaagccg aggatttgtg gccatcacca tgattcccgt gctacgcggg gacccgtgtg
      301 cctgcagctg ctccggtgtt tctgtgtgat tgccccgcag aaacgtactt caacgggatt
      361 ttcgggcgct ttgcctcgct gtcgaaatgt gcctgcagat tgacccagaa ggaaacgggc
      421 agaccgaggg cgctggcaag cgcctcggca atatcctctg tgatgcggat ctcgtccgcg
      481 atcactgcat ctaacgtctc cgctgccaaa cccgtttttt caacgaaact gcttctcgtg
      541 atgccggccc cattgagatg gtcttgcacc aactcccccg gggtgacaac ccagttcggc
      601 ggaaaggcac tcatcggcac cttagccatt gtgataatcc tccactcgat gaatcacgac
      661 gcaggtgacc cggacccagt caagcccgcc gtctgacttc cttgggatcg gttcatttcc
      721 cggcgtaaaa agcaaccgtc ggctcttcga cacgtccatt gccagatatc ccttcaagtc
      781 gtgtttgagc tcatgacacc tggcgctcgg atcttccgaa aaggacaacg cggcgaggtt
      841 cggagccgcc tgcaggtctg tgattcgcct catgatcgcg actgcgcaag gtttcccgta
      901 ctgtctttct agctcccttc tgtcagcgaa gtgcttcgcc actctcttat tcgcatacga
      961 aatctccacc cttttctccc ttaacgctga atttacctca ccggtttccg gaaaccaaat
     1021 ctgcgcccgc gaaactccca gcagttcggt tttttgccgc aatggacgac cgactcccaa
     1081 gtcgggcccg tcagcatatt ccctgagcgt ccggatcatc tgcaaaaacc gacagggctc
     1141 agccgccgcg cacgggcaag gaaccgatca ccgagctgca cccgctgcgt ggaagcttgt
     1201 cgaagtggct ggttctgaaa gcctacacca gcgccggacc tctgctcgtc ccgatccgcg
     1261 ttgcaccggc gcggacgaac gccagcgcct gctcccgcgt tctgatgccg cccgccgcct
     1321 tcacgcccag ccgatccccc accacgcgcc gcataagcgc cacatcctcc accgtggcgg
     1381 cgcccgaaaa ccccgtgcag gtctttacga agtccgcgcc gcactgctcg gcaatgatgc
     1441 aggcgatcgc cttttcgcgg tcggtgagga gaggggcctc gataatcacc ttcaccagcg
     1501 ccggtgtgcc cgacctctgc cgggccgccg cgacaacccc gtcgatgtcc gccgccacct
     1561 cgctccacag gttcgccttc agaataccca ccggcaacac catgtcgagt tcgtgcaccc
     1621 ctcgcgccac cagtgcttcg gcttccgcgc gctttgtctc cggcaatgtc gcgccgtggg
     1681 ggaacccgac caccgccgca acggccacct gcgtcatccg cacccgctcc actgcctgcg
     1741 cggcgaaccg tggatgcacg cacaccgcgg cgaaatggtg ccggatggct tcgtcgcaga
     1801 ggcggtcaac gtcctccggc gtcgcatcgg ggcggagcag cgtgtggtca atcatcgggg
     1861 cgagatccac cgcggcgtcc ccctccgcga gcgaaggtgc cgcctcggat gccccttcct
     1921 ccgctgccag ccgccgcatc acctcggcaa gcacggagtc aaccatctgg ttaatcatgc
     1981 ctcaccgccc atcccgtcga ggattgcggc gatttcgggc gaaatctgcg gttccgtagt
     2041 caaccagatg gttaacacct cccgcatgag agccggcgca acgatgtccg cgcccatgca
     2101 gaggacattt gccgccattc gctcccgtgc cgccttcgct gcggccacat cattgcagca
     2161 ggcagcccgc acgcccttca cccgattggc cacggcggag gcaaccagac cggtctcatc
     2221 caccaccacg ccccagtccg cctgcccgga ggccaccgcg ccggcgactc gtttggcgag
     2281 cagcgccccc ttccgcgccg agccgggaac ccgcaccacc gcctgccccg ccgcgcgcag
     2341 cgcctcgacc agcagcggct ctaccgccga cgtcgccacc acgcccacgg caacgcgcgt
     2401 cgtccgggca gcacgcacgg gttcggcatc cggcgccgac tttccacctt gcgcaccgcg
     2461 cgagcccagc gcggacgcca cggcaatccc gaaccgggcc agcgcatccc tcgccagcgg
     2521 ggtgatgatg gcgccgggcg cgacccggat caccgcctca cccctcgcac gcgcggcaag
     2581 aacgcactcc tccgtcacca ggcgcgggca gtcgcgcgga gctgatgccc ccgcgactgc
     2641 ttcgcgccgg ttgagtgccg ccttcacttc ggctctgagg cgttccagtt cgtccgtatt
     2701 catcgctgcc taaaagctgt actccccgag aatcgcaagg gcgatctgct gggcgcggag
     2761 gccatccgtg attgtcgcgc gggtctgctt ccggccctct tccgccgcct tcacgaaatc
     2821 cgccagttgc gcttcgtaca tgttccactt cttgaacggg tgcacctcgc gccggccgcc
     2881 ctcatgcttc gtcacgagat acccgaattc atcgcggttg aggcccaccg gcgggtcgtt
     2941 atcggggtcg gagaagtaga ggatgccttt cgggccgagc acgttgtgca gtccgccgga
     3001 acgcacgcct gagggcatcc cccaggagtt gagcacggtg tggcggtcgc cggacgcgaa
     3061 ctcgatgatg gcggtgccgg tatcgagcgc ggtgttgacc ggatgcagcc gcaccatgga
     3121 gcccaccacg cgggtcggtt cgccccacat gtagcggagg aaatcgtagt tgtgcaccat
     3181 gccgtcgagc atcgggccgc caccctgatc cgcctggttg aagaacgagc tgccgggccc
     3241 tgggccgccg ccggccatcc accacaccac cggcctgccg atgacacccg cctcgatgag
     3301 ctccttgaac ttgccccact ccgggcaata gcggcgcaca aagccgatct gcgcgacgat
     3361 accagtctct tccaccagcc gcaccgcctc atacgaatcc gccagcgtgc gggagagcgg
     3421 cttttcggtg aagagcggct tgcgcgcgtt gcatgcggcg cggatgccct ccagatggac
     3481 gaactgcggt gtgcacacca ccaccgcgtc cacctccggg tgatccgcca cggcctgcgc
     3541 ggaggacacg atgtgcggga tgctgtagcg ctgctgacgc tccttcatcg tgtccgggtt
     3601 gatatcatac atggcggtaa ccgggatgcc gtgctttttc gcggcgtcga ggtgtgcgcc
     3661 ggcgatgcca ccgacaccga taaaaccgag gcgaacgggc atggtgaacg ctccttgcgt
     3721 aaggatgatg atgcgtgaaa ctggatacgg ccgaatcctg acgatacgac gaaaagatac
     3781 gccctccgcc ggtggtatcc caatcggggg cgagcagcgt gctccgtggt gccggattgc
     3841 cacgcaccca cccgaccctg tgtatgactc aggcaccgag gcgtgttcgt gtcaagggtt
     3901 tt
//
LOCUS       DAOTNG010000291         3895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18692_length_3895_cov_4.223958, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000291 DAOTNG010000000
VERSION     DAOTNG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18692_length_3895_cov_4.223958"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="P5179_12005"
     CDS             <1..221
                     /locus_tag="P5179_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95984.1"
                     /translation="IQIRTAWPDSSSTVNTGGCKGNIRGLGGSLTPDPSPEGRGEKTL
                     TPVHLRGKGSRASRGRDEGASGANLPAG"
     gene            complement(394..1614)
                     /locus_tag="P5179_12010"
     CDS             complement(394..1614)
                     /locus_tag="P5179_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS95985.1"
                     /translation="MLKNPMTDRQRRLKWFRDARFGMFIHWGLYSMLGRHEWVMNRER
                     IPLKEYEKLADKWQPKPNCMRDWAKLAKKAGMRYMVMTTKHHEGFCLFNTKHTDYNAV
                     KRSPGRDLVAEYVDACRAEGLKVGFYYSLMDWHHPDGVKCMKNDASWVRFTEFTHGVV
                     RELCTNYGKVDIMWFDGGWPLSAEGWRSQELINMILDLQPDCIINNRTGLAGDFGTPE
                     NRIVPDKERMWEACMTFNHSWGYTPIDTDYKSAWEVISMLRQCAAGGGNLLLNIGPKP
                     DGSLYHPCDTELERVGRWMMKYGKSIYEATDPIPQDWMITGAFTRKGDTLYYHCNRWP
                     GEELAIGGLRCKILSAKIMGGAVLKFTQKADRLVIKGLPEKAPDVLSTPIELKFEGEA
                     RHELGPGHKLLSEV"
     gene            complement(1638..2654)
                     /locus_tag="P5179_12015"
     CDS             complement(1638..2654)
                     /locus_tag="P5179_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95986.1"
                     /translation="MTPKEIVWRAVHRERPPRLPVSAGSLGVEDRYGVPIHSLFQEED
                     GNRRVDEWGCVWEHTDVPGMGQVKVHPLEDVSKLGAYRFPDYTDDRRYTDVQAALEQA
                     NREEKYVIAGIFLVLFERMHMLHGFENTLVDLYQDRPAMEALADGIVDTHVTLVREMA
                     RRFPGKIDGWTMTDDWGTQQSAFVSFDLWMDFFFPRYSRIFDAMHAAGCDVWVHSCGK
                     VNEIIEGYIRAGADIVNLCQPRALGIEEIGRRYRGRISFESVADIQVTLPTGDRGLIA
                     ADIEALMTHWASPEGGFYFSDYGQGAAIGVNDESIKEFEYDEFSRWSERLYGEPLPPR
                     RNTH"
     gene            complement(2683..>3895)
                     /locus_tag="P5179_12020"
     CDS             complement(2683..>3895)
                     /locus_tag="P5179_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS95987.1"
                     /translation="LPDIVVEEGWDHGAKMGTMAQVPPPIPILGLFRFDRDAVAADAR
                     RMSEAYKGTGGFPFGLAAGDGAFVFFCSSTRNYPVKTLKDIKPCPEHVCRPQWRIHQG
                     RGCPHQCAYCSLGGYLITHVNTEDYIERLADLLAQNPWQKTWLYDDVMDVLTLEPELD
                     TLAPLMRFFERTGDRYLILHTKSDRVHGLIEADAPHNTIIAWSLSGPTQSHRIEPVAG
                     TTESRVEAARQCQDAGMTVRYKFKPIVPIKNWREEAEYTVDLALRRTKPDNLSMTTLM
                     WMDSAELTRCIPEELLDAEFLRAARNAHEEMKDSRVGPYPHAVREEIYRHYLKAIRNR
                     HPDIPVTISTESLEMWKSLGTDLGFTPATYVCGCGAGATPNLLKLQTNPWQDAKVART
                     WDGAPAMPEGE"
BASE COUNT          641 a         1238 c         1218 g          798 t
ORIGIN      
        1 cgattcagat tcggacggca tggccggatt catcctccac ggtgaatacg ggtgggtgta
       61 agggaaatat aagggggttg gggggctccc tcacccccga cccctctccc gaagggcgag
      121 gggagaaaac gctcactccc gtccacctcc gggggaaggg aagccgcgca tcgcgcggca
      181 gggatgaggg agcaagcggc gccaatctac ccgcgggatg aggaagcaag agtggcatca
      241 gcctccctca cctctgctcc ccctcacccc tcctgcgcga tgcgcaggca acccctctcc
      301 cgaagggcga ggggagaaca ggtgcacccc ctcttccacc ctcgggggaa gagggggttg
      361 ttcgccctgc gaacgggggg tgatgaggga acgctacacc tcgctcagca acttgtgccc
      421 cgggccaagt tcgtgccgcg cctcgccctc aaatttcagc tcgatgggcg tggaaagcac
      481 atccggcgcc ttctcgggaa ggcccttgat caccaggcga tcggctttct gcgtgaactt
      541 gagcaccgcg ccgcccatga tcttcgccga caggattttg caccgcaggc cgccgatggc
      601 aagttcttct cccggccacc ggttgcagtg gtagtacagc gtatcgccct tgcgcgtgaa
      661 cgcgccggtg atcatccagt cctgcgggat cggatcggtg gcctcgtaga tgctcttgcc
      721 gtacttcatc atccaccggc cgacgcgctc cagctcggta tcgcacgggt ggtaaaggct
      781 gccgtcgggc ttcgggccga tgttgagaag tagattgccg ccgcccgccg cgcattgccg
      841 cagcatggag atgacctccc acgcggactt gtagtctgta tcgatcggcg tgtagcccca
      901 gctatggttg aacgtcatgc aggcttccca catgcgttcc ttgtccggca cgatgcggtt
      961 ttccggcgtg ccgaaatccc ccgcgaggcc ggtgcggtta ttgatgatgc aatccggctg
     1021 caagtcgaga atcatgttga tcagttcctg cgagcgccac ccctcggcgg aaagcggcca
     1081 cccgccgtcg aaccacatga tgtcgacctt gccgtagttg gtgcagagtt cgcgcaccac
     1141 gccgtgggtg aactcggtaa agcgcaccca ggaggcgtcg ttcttcatgc atttcacgcc
     1201 gtccgggtgg tgccagtcca taagtgagta gtagaacccg accttcaggc cttcggcgcg
     1261 gcacgcgtcc acgtactcag cgacaagatc ccgccccggc gagcgtttta ccgcgttgta
     1321 gtcggtgtgt ttcgtgttga acaggcagaa gccctcatgg tgcttggtcg tcatcaccat
     1381 gtagcgcatg ccggcctttt tcgccagctt cgcccagtcg cgcatgcaat tgggcttcgg
     1441 ctgccacttg tccgcgagtt tctcgtattc cttgagcggg atgcgttcgc ggttcatcac
     1501 ccactcgtgc cggcccagca tggagtagag tccccagtgg atgaacatgc cgaaccgggc
     1561 gtcgcggaac cacttgagcc ttcgctggcg gtctgtcatc gggtttttca gcatggcgtg
     1621 accctcctgt ggatgaatca gtgtgtattt ctgcgtggcg gaagcggttc gccgtagagg
     1681 cgctccgacc accggctgaa ctcgtcgtat tcgaactcct tgatggattc gtcgttgacg
     1741 cctatggcgg ccccctgccc gtaatcgctg aagtagaagc cgccttcggg ggatgcccag
     1801 tgcgtcatga gggcttcaat gtcggccgcg atcagccctc tgtcgccggt gggcagcgtc
     1861 acctgaatat cggcgacgct ttcgaacgaa atgcgtccgc ggtagcgtct gccgatctcc
     1921 tcgattccca gcgctctcgg ctggcagagg ttcacgatat ccgcccccgc ccggatgtag
     1981 ccctcgatga tctcgttcac cttgccgcac gaatgaaccc agacatcgca cccggcggcg
     2041 tgcatggcgt cgaagatgcg cgagtaccgg gggaagaaga aatccatcca gaggtcgaag
     2101 ctgacaaacg cgctttgctg ggtgccccag tcgtccgtca tggtccagcc gtcgatcttt
     2161 ccggggaagc gccgcgccat ttcgcggacg agcgttacat gcgtgtccac gatgccgtcg
     2221 gccagcgcct ccatggcggg gcggtcctgg taaaggtcga caagcgtgtt ttcgaagccg
     2281 tggagcatgt gcatgcgctc aaacagcacg agaaagatgc cggcgatgac gtacttctct
     2341 tcccggtttg cctgctcaag cgcggcctgg acgtcggtgt accggcggtc gtcggtgtag
     2401 tccgggaaac ggtacgcacc gagtttcgag acgtcctcga gcgggtggac cttcacctga
     2461 cccatccccg gcacgtcggt gtgctcccag acgcatcccc attcgtctac ccgcctgttc
     2521 ccgtcttcct cctggaaaag gctgtggatg gggacgccgt accggtcttc cacccccagt
     2581 gaccccgccg aaacgggcag ccggggcggc ctctccctgt gcaccgccct ccagacaatc
     2641 tcttttggcg tcatcgacat ctccggggtt cttgggcccg tgttactccc cctccggcat
     2701 ggccggtgcg ccatcccatg tgcgcgcaac cttcgcatcc tgccacgggt ttgtctggag
     2761 tttgagcagg ttcggggtcg cgccggcgcc gcagccgcag acatacgtcg ccggggtgaa
     2821 gccgaggtcc gtgccgaggc tcttccacat ttcgaggctc tccgtcgaga tcgtcaccgg
     2881 aatatccggg tgacgattcc ggatggcctt gaggtagtgc cggtagattt cctcgcgcac
     2941 cgcatgggga tacggaccga cgcgcgaatc tttcatctcc tcgtgcgcgt tgcgcgccgc
     3001 ccgcaggaac tccgcgtcga gcagttcttc gggaatgcac cgggtcagct ccgctgagtc
     3061 catccacatg agcgtcgtca tgctgaggtt gtcgggtttg gtcctccgca gggccaggtc
     3121 cacggtgtat tccgcctctt cgcgccagtt tttgatcgga acgatgggct tgaacttgta
     3181 gcggactgtc atcccggcgt cctgacactg ccgtgccgcc tctacgcggc tttccgtggt
     3241 gccggcgacc ggttcgatcc ggtgcgattg cgtcggaccg ctcaggctcc acgcgatgat
     3301 ggtgttgtgc ggggcatccg cctcgatcag cccgtgcacg cggtcgcttt tggtgtggag
     3361 gatgaggtag cggtcaccgg tgcgttcgaa aaaccgcatg agcggcgcca gcgtgtcgag
     3421 ttccggctcc agcgtcagca cgtccatcac gtcgtcgtag agccaggtct tttgccacgg
     3481 gttctgcgcg agaaggtcgg ccagccgttc gatgtagtcc tctgtgttca cgtgggtgat
     3541 gaggtatccg ccaagggagc agtacgcgca ctgatgcggg catccgcggc cttggtggat
     3601 gcgccactgc ggccggcaga cgtgttcggg acagggtttg atgtccttca gcgtcttgac
     3661 cgggtagttc cgggtgctgg agcagaagaa cacgaacgcg ccatcgcccg ccgcgaggcc
     3721 gaaggggaag ccacctgtgc ccttgtaagc ttcgctcata cgtctcgcgt ccgccgccac
     3781 ggcgtcccgg tcgaaacgga acaacccgag gatggggatc ggcggtggaa cctgcgccat
     3841 agttcccatc ttcgcgccgt gatcccaccc ctcctcgaca acgatgtcgg gcagt
//
LOCUS       DAOTNG010000292         3894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18699_length_3894_cov_4.235218, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000292 DAOTNG010000000
VERSION     DAOTNG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18699_length_3894_cov_4.235218"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5179_12025"
     CDS             complement(<1..210)
                     /locus_tag="P5179_12025"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006587231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRS95988.1"
                     /translation="MQRIGVMTSGGDAPGMNANVRAIVRTGIFRGVQVFGIRDGFAGL
                     ISDEIIPMTSPSVGGIVGRGGTILGT"
     gene            437..922
                     /locus_tag="P5179_12030"
     CDS             437..922
                     /locus_tag="P5179_12030"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRS95989.1"
                     /translation="MDPKPVSDSSVTMTELVLPSHANPLGTVFGGVIMSWIDICAAIA
                     AQRHARRVVVTASIDMLHFRSPITVGCVVHLKAQVNHAWHSSMEVGVRVDGENPLTGE
                     TRHAATAYLTYVALDEKRRPVEVPAVLCETPEQERRSRDAQARREKRLENRSISEYES
                     R"
     gene            complement(984..2042)
                     /locus_tag="P5179_12035"
     CDS             complement(984..2042)
                     /locus_tag="P5179_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS95990.1"
                     /translation="MTPRQRVEAALRGEWADQVPFTIYANKLPRCQVELELRNAGVCV
                     LERRSVVRAETPNVRVTSIGYEKGGVQFTRRVFSTPRGELSTIERPGIGTTWRVKRMF
                     TRPEDYAPLLALIKDQRYVPDFETFQKMREDLGEGAYLRAGIGYSPLQEIIYTYMGVE
                     CFSIEWAERRDEVMRLYDALTEDRRKIYPLLAESPAEIINYGGNVSVEVVGKDRFERF
                     IIPHYNEVAEIVHKRGKMLGSHLDGNNRQLAPLVANSLLDYVEAFTPPPDCDMTVAEA
                     RELWKGKVLWINFPSSVHLSGVEAVENMMHRLLREAAPGDRFLVGVTEDVHEDAWRKT
                     FPAMARILNKSGRLPLER"
     gene            2234..>3894
                     /locus_tag="P5179_12040"
     CDS             2234..>3894
                     /locus_tag="P5179_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRS95991.1"
                     /translation="MPGFEGRGGGHHYGMGPVSGPVLPGGDIGNGVAGLPESVRAAFL
                     KSAEDGEQILVAATSDLSPDGRYGQQHVLASKNRLCLAVPNGDQPNLSMWPLKELKKA
                     NVDLLVGQASLVATIGKDRVELCRFTNSKQREFHRLAALLEELARNGAMPEEKADPKD
                     SLPRYCSRCSRLLPEPGGTCPACLKRGQVLIRLLGYLRPHWKRAALLIATMVVSALIE
                     TLPPYLTKVLIDDVINRPHVTTIAGWSADHLGWSRVAWLALIVTLFLLSRILLVGVGI
                     LGGRQATWLGPRIVADLRAKLYNHLQRLSLNYFDKSRTGALISRVTHDTAHVQGFLVN
                     GVPHVGIDLFLLVFIGMILFSMNWQLTLAILVPLPIVIYVSKNFWRFMRSLFGRAWAR
                     SARLVATLNDSLNGIRVVKAFGQEEQEIARFNSRNWDLVSAETRAEMTWATFFPAITF
                     LTMLGQFIIWYVGGSNVIDGTMSLGTLLAFFSYLGMFYRPIQMIARMNEWLTRDLTAA
                     ERVFEILDTAPDVKDSPNPIQLNEIRGEIRLENVRFGYDPLRPVL"
BASE COUNT          806 a         1164 c         1070 g          854 t
ORIGIN      
        1 agttccgagt atcgtaccac ctctgccgac tatgccaccg acggaaggcg acgtcatcgg
       61 gatgatctcg tcggaaatga gcccggcgaa tccgtcccgg attccgaata cctgaacgcc
      121 tctgaatatt ccagttctga cgattgcgcg tacgtttgcg ttcatcccgg gagcgtcacc
      181 tcccgacgtc atcacaccga tcctctgcat ctgtttctcc gcgattgttc aacctcggtg
      241 ccggtgcgta gccggttcac tccggttgcc gtgaacaccg gagcaaacgc cagcgccgga
      301 caaaaatcag caaaacagaa gagcccgcgg cggccgtgct accgttggtg ccttggcatc
      361 tgttccacaa acacgcaatt tcaacgatac tgaaatacgt cacgcgccgg caaacctgtt
      421 tccacggaac cattgcatgg atcccaaacc cgtttctgat tcctcagtca ccatgacgga
      481 actcgtgcta ccgagccacg caaatcccct gggaaccgtt ttcgggggcg tcataatgtc
      541 atggatcgac atctgtgccg ccattgcggc gcaacgccac gcaagaaggg tcgtggtgac
      601 tgcttccatc gacatgctcc actttcgttc cccgatcacc gtcggctgcg tggtacacct
      661 caaggctcag gtcaatcacg cctggcattc gtccatggaa gtcggagtga gggtcgatgg
      721 ggagaacccg cttaccggcg agacgcgcca tgccgcgact gcctacctga cgtacgtggc
      781 cctcgatgag aagcggcgcc ctgtggaggt tcccgctgtt ctgtgtgaaa cacccgagca
      841 ggaacgacgg tcgagggacg cccaggcgcg gcgagaaaag aggctcgaga accgatcgat
      901 cagcgagtac gaaagccgtt gactggtgaa acaaccggac aggttacaag gcgccttggc
      961 ctgaccgaat tccgagatgt ggttcaccgt tccagtggca accttcccga tttgttgaga
     1021 atccttgcca ttgcaggaaa cgtttttcgc cacgcgtctt cgtgcacgtc ttcggtcaca
     1081 cctacgagga aacgatcgcc cggagctgct tccctgagaa ggcggtgcat catgttttcg
     1141 accgcttcca ctccggagag gtggaccgag gatgggaagt tgatccacag tactttgcct
     1201 ttccacagct ctcgggcttc agcaaccgtc atgtcacagt cgggaggagg cgtaaatgcc
     1261 tcaacgtaat cgagtaatga gttcgccacc aacggcgcaa gctgccggtt gtttccgtca
     1321 agatgcgacc cgagcatttt gccccgtttg tgaacgattt ccgccacttc gttgtaatgc
     1381 ggaatgatga agcgctcaaa gcgatccttc cccacaacct cgacgctcac gttgccaccg
     1441 taattgatta tttctgccgg actttccgcc agtaacgggt agattttcct tctgtcttcg
     1501 gtcaacgcgt cataaagacg catgacttcg tcccgccgtt ccgcccactc gatgctgaag
     1561 cactccacac ccatgtaggt gtaaatgatt tcctggaggg gagagtaacc gattcccgct
     1621 cgcagatatg cgccctcgcc aaggtcttcc cgcattttct ggaaagtttc gaaatcgggc
     1681 acgtaccgct ggtctttgat cagcgcgaga agcggggcat agtcctccgg tctcgtgaac
     1741 atgcgtttga cgcgccacgt agtgccaata ccgggccgtt cgatggtgga cagttctccg
     1801 cgtggcgtgc tgaaaacccg tcgcgtgaac tgcactcctc ctttctcgta cccgatagag
     1861 gtcactctga cgttgggtgt ctcggcgcgc accacactcc gtcgttccag cacgcacacc
     1921 ccggcgttcc gcagttccag ttccacctgg cagcgaggga gtttgttggc gtagatggtg
     1981 aaaggaacct ggtccgccca ttctcctcgc aacgcagctt ccactctctg ccgcggggtc
     2041 attttcgcca cgtgctaacc ctccactggc gtgacagaac attgcagtga cgagaatacg
     2101 gctcccacgc cagcaacgca atcactgatg tctggaacat tgggacactc cgttcaaagc
     2161 gatatcttat ctgtataacg cgaaacaatg cgaagactcg ttcgcagggg tttctccgaa
     2221 ccgagaggtt ggcatgccag gtttcgaagg gcgaggcggg ggacatcact acggcatggg
     2281 acccgtttcc ggacctgttc ttcccggagg tgatatcggc aacggcgtgg ccggtctgcc
     2341 ggaatccgtg cgcgctgcgt ttctgaaatc cgctgaggac ggggaacaga tcctcgttgc
     2401 ggccacaagt gacctgtcac cggatggccg atacggccag caacacgttc tggcaagcaa
     2461 gaaccgtctg tgcctggctg taccgaacgg tgaccagcca aatctttcaa tgtggccgct
     2521 caaggagttg aaaaaagcca acgtagacct tcttgtggga caggcttcgc tggtggccac
     2581 gattggcaag gacagggtgg agctctgtcg tttcacgaac tccaaacaac gggaattcca
     2641 ccgactggcg gcgctccttg aagagctcgc gcgcaatggc gcgatgccgg aagaaaaggc
     2701 tgacccgaaa gactcgttac cacggtattg ctcaaggtgc tcccgccttt tgccggaacc
     2761 cggcggcacg tgccccgcgt gcctgaagag aggtcaggta ctcattcgct tgttgggata
     2821 cctgcgtccc cactggaaac gggctgcatt gctcatcgcg acaatggtcg tctcggcgct
     2881 gatagaaacc ttgccgccgt acctgactaa agttctgatt gacgatgtaa tcaatcgtcc
     2941 ccacgtgacg actatcgccg ggtggagtgc cgaccatctg gggtggtcac gggtcgcgtg
     3001 gctcgcactc atcgtcaccc ttttcctgct ctcgcgcatt cttctcgtcg gcgtgggcat
     3061 ccttggcggg cggcaggcga cgtggcttgg ccctcgcatc gtcgctgatc tccgggcgaa
     3121 gctctacaac cacctccaac gactcagtct gaactatttc gacaagagtc gcaccggcgc
     3181 tctcataagc cgtgtaacac acgacactgc ccatgttcag ggattcctgg tgaacggcgt
     3241 gcctcacgtg ggcatcgacc tgtttctgct tgtgttcatc ggaatgatac ttttctcgat
     3301 gaactggcag ctcacactgg ccattctggt gcctcttcct atcgtcatct atgtcagcaa
     3361 gaacttctgg cggttcatgc gttcgctctt cggaagagca tgggcgcgaa gtgcgcgcct
     3421 cgtggcaacg ctgaatgact cgctcaacgg tattcgcgtg gtgaaggcct ttggccagga
     3481 agagcaggag atcgcccggt tcaactcaag gaactgggac ctcgtctccg ccgaaacacg
     3541 cgccgaaatg acgtgggcaa cattcttccc tgcgatcacc ttcctgacga tgctcggaca
     3601 attcatcatc tggtatgtcg gcggtagcaa cgtcatcgac ggaacgatgt cccttgggac
     3661 tctcctcgcg ttcttcagct atcttggcat gttctaccgg ccaatccaga tgatcgcacg
     3721 gatgaacgag tggttgacgc gtgatcttac cgctgcggaa cgggtgttcg aaatcctcga
     3781 cactgctcct gatgtgaaag acagccccaa cccgattcaa ttgaacgaaa tacgtggcga
     3841 aatccgactg gagaacgtgc gtttcgggta cgaccccctt agaccggtgc tcaa
//
LOCUS       DAOTNG010000293         3891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18722_length_3891_cov_4.622002, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000293 DAOTNG010000000
VERSION     DAOTNG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18722_length_3891_cov_4.622002"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1407
                     /locus_tag="P5179_12045"
     CDS             319..1407
                     /locus_tag="P5179_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS95992.1"
                     /translation="MKKSILCLAADGLAVLCLFAHGATAKTDSLTTGQRIQRVFPFQV
                     GQQYEFQSGVTFHRPTGQALPPYTIAQITITDTVIDGKTYLHIPYWSSFGTEYYRLDD
                     SLRIRNYNPDSSKEEVFLNLGYGGTLMFPGMGFECLGTCRMSGYSRSGSFTGIDKPFH
                     LAYWVYYDNPSYTKLTVAYQAWTTASRTTTETGITTDADSALAYGFDLCCESTSVTNI
                     FGVGGDGSVQHRQGVTDRQRRLAVFRPVSNEPWLPLANAMGDYADVRTENNRPSQFSV
                     SAYPNPFNTNTLVRYSLPTKGHVTMAIYSIEGRLVRTLVDRALAAGTHETQWDGRGND
                     GKEAGGGVYVVRLVTPTNAVVERISLIR"
     gene            1430..>3891
                     /locus_tag="P5179_12050"
     CDS             1430..>3891
                     /locus_tag="P5179_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS95993.1"
                     /translation="MWQILATLVFGVGLPVCSTAQPAATTSSIGGAEVPSARQRGLKL
                     VLGNAGFNRLTVEQRENLLSGRTMCATSYLAKSEQPYRTARPSRVASYADLPTVGDHL
                     QNKKYRLVLIFIATAGHDSARPVLIRNSFLPGRVPATTSSTPNWMQNDTLSYDTYVKD
                     AIEYFFDKNSYGALDVDVVIPYPPNSSSPIWVSPDTAGYGVVDSVLKRIETSYPDSFF
                     YDVSPPSPYDYQRVGYLFTDTFATGWCSSDSNELWASEAIRDSIWDGATFAGAAWAGT
                     GNPLWPDVHSETFAWQIIHEMLHHITYANASHPSFADRGVREFGTRTWGFDIMDHGGK
                     ACATNGHYGAHALAPIDKYLLGFIEGNWLRTITTNTTDVRLSPNHVSPPSGGRLLARI
                     PLSSGTSGTESFVVSNHQGIGIDEVYNPGMGDSLNNGLHIWHIGDGFELGFQSNRADV
                     EVAWTIALGDTFCHPEGQSHNPSSWAVSQYLPTEYSDNFDWIDGVLYDSPLYPSMGGK
                     GLYILNQAYGGWSAGGPGIGNSTAFCNHNSDSGTLRWEHLYDRNIITPTINDFFRPGN
                     AFTPYSRPNTDKYHRYAVTSYADSMVCTQGEPQEDRPRDGNIMLPLGGAPFVSSQLRD
                     VNDGLTRVGIVNIRQETNGDMLFDVYFNFWEGEFNDARNFGYLKRYDDGTECESGSVC
                     WRNVWESMTLAGVAHDTVVVGPNGFLINTGRKITVKTGTTMKVLGPITVEGTLAVESG
                     VIFDTIKGDIVIASGGRLELGSNVTLPFDLDHCKDLHVDSAGTLIGSGPTSVIEGPIN
                     VRGRSATVQNVKVLFADPYGTG"
BASE COUNT          890 a         1078 c         1071 g          852 t
ORIGIN      
        1 cttgacaggg aacctttctt ttcgtactga gttggtgtat aataggaggt agcaccgcac
       61 cttgcatggc acccgctgaa gagaggaggc gaaccatgcc tttcttcgtt tcatctcttc
      121 cccctccggg ggggggggta ccgcgagcgg cgcgcacggg ccgcgccaac gaacacgcct
      181 gaaacgccca cgagcacgct gccagcctac acgacctcca taaacaacct tgaaatagat
      241 tcgcgcccct gaaggggttt ttgtacgttg ttcgtggtgg cgaccgcgcg gggttttgac
      301 ttcagaggag aagcaaccat gaaaaagagt atcctgtgtc tcgcggcgga cgggcttgca
      361 gttctttgtc tgtttgcgca cggtgcaacc gcaaaaacgg atagcctcac aacgggacag
      421 cggattcaac gcgtgtttcc gttccaggtt ggccaacaat acgagttcca gtctggtgtc
      481 acgttccatc gtcccaccgg ccaggccctt cctccctaca ccatagccca gatcacgatc
      541 acggacacgg ttattgacgg caagacctat ctccacatcc cctattggtc gagctttggc
      601 actgagtatt atcggctcga tgactcactg cggataagga actacaaccc cgacagcagc
      661 aaggaagagg tatttctcaa cctcgggtac gggggaacac tcatgtttcc gggcatgggg
      721 tttgagtgcc tcgggacgtg ccgaatgagc ggctactcgc ggagcggaag cttcacgggg
      781 atagacaaac cttttcatct tgcctattgg gtttactacg ataaccccag ttataccaag
      841 ctcaccgtcg cctaccaggc atggacaaca gcgtcacgaa caactacgga gacgggaatc
      901 acaacggatg cggactccgc cctggcgtac gggttcgatc tctgctgcga gagcacttcg
      961 gttacgaaca ttttcggtgt cggcggcgat ggttcggttc aacaccggca gggagtcact
     1021 gaccgacagc gccggttggc ggtattccga cccgtgagca atgagccatg gcttccgtta
     1081 gcgaacgcaa tgggcgacta tgcagacgtc cgcaccgaga acaatcggcc ttcccagttc
     1141 agtgtatccg cctatcccaa cccgttcaac acaaacacgc ttgttcgcta ctccctgccg
     1201 acgaaaggcc acgtcacaat ggcgatttac tcaatcgaag gccggttggt gaggacgctc
     1261 gtcgacaggg cattggcagc gggtacgcat gaaacccaat gggacggccg tggaaatgac
     1321 ggaaaagaag caggaggcgg agtctatgtt gttcgtttgg tgacgccaac gaatgccgtt
     1381 gtcgaaagga tttccctgat cagatgaaca gaaaaagggg gagagtgtca tgtggcaaat
     1441 ccttgcaact ctcgtgttcg gtgttggtct tccggtctgc tccaccgcgc agcccgcagc
     1501 gacgacctct tcgataggag gggctgaagt accctccgca cgccagcgtg gcctgaaact
     1561 ggtgctcggt aacgcagggt tcaaccgcct cacggtcgaa caacgcgaaa accttctcag
     1621 cgggcgcacg atgtgcgcca cgagttacct cgcgaaaagt gaacaaccct atcgcacagc
     1681 gcggccttct cgcgttgcca gctatgctga ccttcctacc gttggcgatc atctacagaa
     1741 caaaaaatac cgcttggttc tgatcttcat tgctacggct ggacacgatt ccgcacggcc
     1801 tgttttgatc cgcaacagtt ttcttccggg gcgcgttcca gcaacgacat cttcaacccc
     1861 gaactggatg caaaacgaca cacttagtta tgacacatat gtcaaagatg cgatcgagta
     1921 cttcttcgac aagaactcct atggtgctct cgatgttgat gttgtgatac cgtatccacc
     1981 aaactcatct tcgcccatct gggtatcgcc tgataccgca ggctatggcg tagtcgacag
     2041 cgttctcaaa cgcatagaaa cttcgtatcc agattcattt ttctacgatg tatccccccc
     2101 gtcgccgtat gattaccaac gggttggcta cctattcacc gatactttcg ctacagggtg
     2161 gtgttcttcg gatagtaacg aactctgggc aagcgaagcg attcgcgatt ccatttggga
     2221 tggcgcaaca ttcgctgggg ccgcgtgggc gggaacaggt aatccgttgt ggcccgatgt
     2281 gcattctgaa accttcgcct ggcagatcat ccatgaaatg ctccatcata tcacctatgc
     2341 gaacgcctca catccttcgt tcgccgatag aggggtccgg gaattcggaa cgcgaacgtg
     2401 gggattcgac atcatggacc atggcgggaa agcgtgcgcg acgaacgggc actacggggc
     2461 acatgcgctc gctccgatag acaaatacct tcttgggttc atcgagggga attggctcag
     2521 gacgataaca actaacacaa ccgacgttcg cctgagcccc aaccacgtca gtcctccgtc
     2581 ggggggcaga cttttggcgc gtatcccgct cagctctggc acgtccggca cggaatcttt
     2641 cgtggtttca aaccatcagg ggattggcat agacgaagtg tacaacccag ggatgggaga
     2701 ttctctcaac aatggattac acatttggca tatcggggac ggctttgaac tcggctttca
     2761 aagcaaccga gctgatgtgg aagttgcctg gacgatcgcc ctcggcgaca cgttttgtca
     2821 tcctgaaggt caatcacata acccctcctc gtgggcggtc tctcagtact tacccactga
     2881 gtactccgat aattttgatt ggatagatgg cgtgttatat gattcccccc tttacccttc
     2941 catgggcgga aaagggttgt atatcctcaa tcaggcgtac ggcggatgga gcgcgggagg
     3001 gccgggaata gggaacagca cggcgttttg caaccacaac tccgacagcg gcacgctgcg
     3061 ttgggagcat ctctacgacc ggaacatcat cacaccgaca atcaacgatt tcttccgccc
     3121 cggtaatgcg ttcacgccgt attcgcgccc gaacacggac aaataccacc gatacgccgt
     3181 gacatcgtat gcggactcca tggtatgcac gcaaggcgaa ccgcaggagg accgcccgag
     3241 agacggaaat atcatgctcc cgttaggcgg cgctccgttt gtttccagtc aactgcggga
     3301 cgtcaacgac ggtttgacaa gggttggtat cgtcaatatc cgccaggaaa cgaatgggga
     3361 catgttgttc gatgtgtact tcaacttctg ggaaggcgag ttcaacgatg ccagaaattt
     3421 cggctacctg aagcggtacg acgacggcac ggaatgtgaa agcggctcgg tgtgttggcg
     3481 caatgtctgg gagagcatga ccttggcagg tgtcgcgcac gacaccgttg tcgtaggccc
     3541 gaatgggttc ttgatcaata ccggtcggaa gatcaccgtg aaaacgggaa cgactatgaa
     3601 ggtacttggg cccatcacgg tcgaaggtac gttggcggtt gaatcaggtg ttatttttga
     3661 cacaatcaaa ggcgacattg tcatcgccag cggcgggcgg cttgagttgg gatcgaatgt
     3721 caccctccca tttgatttgg atcactgcaa agaccttcat gtggattcag caggcacact
     3781 gattgggtca ggtcccacaa gcgtcatcga aggcccgatc aatgtccgcg gtcgttcggc
     3841 cacggtgcag aacgtcaagg ttcttttcgc tgacccctac ggtaccgggc a
//
LOCUS       DAOTNG010000294         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18778_length_3882_cov_4.949569, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000294 DAOTNG010000000
VERSION     DAOTNG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18778_length_3882_cov_4.949569"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="P5179_12055"
     CDS             complement(<1..999)
                     /locus_tag="P5179_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRS95994.1"
                     /translation="MAAEEKRPVLGIDLGGTKVLFGVVDKNGRVLTRLKVKSATTTSE
                     EIVTQLVVGIEEIRRKSDKEIAAVGIGVPGTVDTGSGHVVATANLPLNDVPLGKRLTE
                     LCGLPVVVGNDVTLCTLGEAWYGAGAEVDDLFGIFIGTGIGGGIIIDGKLYAGAHGGS
                     GEVGHIRVAQEGPVCGCGGTGCLESFASRTAIERDIRKAVEAGTATVLTDAVRSGERL
                     RSGMLRSALQEGDKLVSGILAKAAELIGLGIANVVNVLDPEVVVVGGGLIEACGDFMM
                     PIIVDSAKPRMMKVYGEPASIVRSYLGDDAGIVGAAVIAKEALKARRPAGATAYVPQ"
     gene            complement(1041..2120)
                     /gene="ychF"
                     /locus_tag="P5179_12060"
     CDS             complement(1041..2120)
                     /gene="ychF"
                     /locus_tag="P5179_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRS95995.1"
                     /translation="MELGITGLPLTGKTTLFNALTGEQRATGGFSSDKPSHLAVVKVP
                     DERLDVLTAMFRPKKTVPADVKYVDVAGIVKGSAKDSRSGVLNALRTVDALIHVVRAF
                     ESDSVPAPEGGINPVRDVGDLDLEMVLADLEVAERRLDRLEKELKAGRKEGEREWTLL
                     RSCRDALESEMPLRELNLTDEEEKLLRGFQFLTRKPIVVVVNVGEDQLGNESYRSTEL
                     ASLATRAKTELIVLCAEAEMQISRLDAADQPAFLEALGISEPAVRRLVKVSYRLLDLI
                     SFFTVGPDEVRAWTVRSGALAPEAAGVIHTDLERGFIRAEVITYDDLVECGSLPHARE
                     KGLLRLEGKAYEVRDGDILNIRFSV"
     gene            complement(2363..3112)
                     /gene="surE"
                     /locus_tag="P5179_12065"
     CDS             complement(2363..3112)
                     /gene="surE"
                     /locus_tag="P5179_12065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRS95996.1"
                     /translation="MRVLLTNDDGYFATGISVLRRVLSRNPDLDVVVVAPDREQSGSS
                     HALTLHRPLRFDEVEPGVYKVDGTPTDCVLLATRGGLPEPPEAVVSGINAGANMGDDV
                     TYSGTVAAAMEGTLLGLPSLAVSLVGGTHFETAAEIAAKLLSEACTKGLPRETLLNVN
                     VPDCSRDRLKGVRVTRQSKRTYHDEVIAKRDPRGRAYFWIAGDTPTWVASDDSDYAAV
                     MSGFVSVTPLHLDLTHHAAIEALADWNLELP"
     gene            complement(3112..>3882)
                     /locus_tag="P5179_12070"
     CDS             complement(3112..>3882)
                     /locus_tag="P5179_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS95997.1"
                     /translation="DDGVDGIVLVNAIRRLSFEGGSATVGAGYEWDSLVEASVSRGLG
                     GIAGMSGIPGTVGGAVCGNAGAYGESVGDRVISVSVVDREGQLREIEGAGLGFCYRGS
                     ALKMSGEPIVRVRLALRPEDRRALSELRQSVLASRAEKHPPKTALTAGSFFKNIDDRG
                     ERARLAIALDLATHERRLAAGLLLDRAGARGLRVGDAEVYSKHANIIVNRGHATAAEV
                     NRLAVLMKEKVRHAFGVRLEREVVWLGRGNPDGDPTGE"
BASE COUNT          786 a         1305 c          990 g          801 t
ORIGIN      
        1 ctggggaacg tacgcggttg ctcctgcggg gcgccgcgct ttcagcgcct ctttggctat
       61 cactgccgcg ccaacgatac cggcatcatc tccaaggtaa gaacgcacga tggatgccgg
      121 ctcaccgtac actttcatca tccggggttt cgccgagtcc acgattatcg gcatcatgaa
      181 atccccgcac gcctcgatga ggcccccacc aaccacaacg acctcaggat ccagcacatt
      241 caccacattc gcgattccga ggccgatgag ttccgcggcc ttcgcgagga tgccagagac
      301 cagcttgtcc ccttcctgca gcgccgatct cagcatgccg ctccgcagtc gctcccccga
      361 ccttactgcg tctgtaagaa ccgtcgccgt gccggcctcc accgcctttc tgatgtcccg
      421 ttctatcgca gtccggctgg caaacgattc gagacaaccc gttccgccgc agccacaaac
      481 cgggccttcc tgtgcgacgc ggatgtgtcc gacttccccc gatccgccgt gggcaccggc
      541 gtacagcttt ccatcgatga tgattcctcc gccaatcccc gttccgatga atattccgaa
      601 caaatcgtcc acctcggcgc ccgccccgta ccaggcttcg ccgagcgtgc agagcgtcac
      661 gtcattgcca accaccaccg gaaggccgca gagttccgtc agacgcttac ccagcggcac
      721 gtcattcagc ggaaggtttg ccgtggcaac cacatggcca ctccccgtgt ccactgttcc
      781 gggaacccct atcccgactg ccgcaatctc cttgtcactc ttgcggcgga tttcttcgat
      841 accgacaacc agttgcgtta cgatctcctc agatgtcgtg gttgcggatt tcactttcaa
      901 acgtgtgaga acacgtccgt tcttgtccac cactccgaac aggactttcg ttccgccgag
      961 gtctatgccg agaaccggac gcttttcttc cgccgccatc gcaggttcct ccctgagaaa
     1021 cacacgcctc tcagcaaaag tcagacgctg aaacgaatgt tgaggatatc gccgtccctg
     1081 acctcgtatg ccttcccctc aaggcggagc aaacctttct cgcgtgcgtg tggcaatgat
     1141 ccgcattcca cgagatcgtc gtacgtaata acctccgccc ggatgaatcc ccgttcaaga
     1201 tccgtgtgga tcacacccgc cgcttcgggt gccagagctc ctgaccttac tgtccatgca
     1261 cggacttcgt cgggtccaac ggtaaaaaag cttatgagat cgagaagtcg gtacgacact
     1321 ttcaccagcc tcctcaccgc cggttcagaa atgcccagcg cctccaggaa agccggttga
     1381 tcagccgcat ccagtcggct gatctgcatc tccgcttccg cgcagagcac aatcaattcc
     1441 gttttcgcgc gcgtggcgag ggaagcaagt tccgtcgaac ggtagctctc gtttcccagc
     1501 tggtcttcgc ccacattcac aaccaccaca atgggtttcc tggtgagaaa ttgaaaccct
     1561 cgcagcagct tttcctcttc atccgtaaga ttcagttcgc gaagcggcat ctccgattcg
     1621 agcgcatctc ggcaggaacg aagcaatgtc cactcgcgct ccccttcttt ccttcccgcc
     1681 ttcagctctt tctccagacg gtcaagcctg cgttcggcaa cctccagatc tgccagcacc
     1741 atttcgagat ccaggtcgcc cacgtctcga accgggttga tcccgccttc aggggcgggt
     1801 acggagtcag attcgaaagc ccgaacgaca tgtatcagtg catcaactgt tcgcagagcg
     1861 ttcagcacgc cacttctgga atctttcgca gaacctttga caatccccgc aacgtcaaca
     1921 tacttcacgt ccgcgggtac cgtctttttc ggacgaaaca tggcggtaag aacgtcaagt
     1981 cgttcatccg ggaccttcac caccgcaagg tgcgagggtt tgtcggacga gaaccctccc
     2041 gttgcacgct gttcgcccgt aagcgcattg aaaagcgttg ttttccctgt caagggcaat
     2101 ccggtaatac cgagttccat ctcgccttct ctctcggaaa cgttccccgg ccggaaaaga
     2161 gcatcctcac aactcggtcg gggcagattt ctacagaatc taccggactc ggaacgggtt
     2221 tccaacagaa cttggtatgc ggggcacgtt ggcggaggta ttcccgctca ggcctgtcgg
     2281 tcagagggat tcccgtttgt cacaacttgg gacccgggcg aagcccgcgt ctccctcggg
     2341 aggtcaggaa acgacaccca ggtcagggaa gttcgaggtt ccagtcggcg agggcttcga
     2401 tcgccgcgtg gtgcgtcaaa tccagatgga gcggcgtcac cgatacaaaa cccgacatta
     2461 cggcggcgta atcgctgtcg tcgcttgcaa cccaggtggg agtgtcccct gcgatccaga
     2521 aataagcccg gccccgtggg tcccgtttcg cgatcacttc atcatggtag gttctttttg
     2581 attgccgcgt cacccgcacg cctttgaggc ggtccctgga gcagtcgggg acattgacgt
     2641 tcaacaatgt ctcgcgggga aggcctttcg tgcacgcctc ggaaagcagc ttggctgcaa
     2701 tctctgctgc cgtttcaaaa tgcgtcccgc caacgaggct cacggccaga gaaggtaagc
     2761 cgagaagtgt cccctccatc gccgctgcga ccgttccaga atacgttaca tcgtccccca
     2821 tattggcacc ggcgttgata ccggatacga cagcttcggg tggctctgga agtccgccgc
     2881 gggtggccag aagtacgcaa tcagtggggg ttccgtcaac tttgtacacg cccggttcaa
     2941 cctcgtcgaa cctcaaagga cgatgcaaag tgagtgcatg gcttgatccg ctttgctcac
     3001 ggtccggcgc cacaaccaca acatccagat cagggttgcg cgagagtacg cgacgcagca
     3061 cactgatccc ggtcgcaaaa tacccgtcat cattcgtcaa cagaacccgc atcattctcc
     3121 cgtcgggtcg ccgtcaggat ttccacgacc cagccaaacg acctctcgtt cgagccgcac
     3181 accgaacgca tgccggacct tctccttcat gagtaccgcc aaccggttca cttcggcggc
     3241 ggtcgcatgc ccgcgattca ctatgatgtt cgcatgtttc gaatacactt cggcatcccc
     3301 gacgcgaagt ccgcgggcac ccgcccgatc gagaagaaga cccgccgcca gtcttcgttc
     3361 gtgtgtggcc aggtccagtg caatcgcaag gcgcgctcgc tcgccccggt cgtcaatgtt
     3421 tttgaagaag gaacccgccg tcagtgcggt ttttggagga tgtttctccg cgcgggaagc
     3481 gagaaccgac tgccgcagct cggaaagggc ccgacggtcc tccggccgga gcgccagccg
     3541 gacacgaacg ataggttctc cgctcatttt gagcgcgctc cctcggtaac agaatccaag
     3601 gcccgctcct tcgatttcgc gaagttgccc ctcccgatct accacgctga cagaaataac
     3661 tctgtccccc accgattcac cgtaggcacc cgcatttccg cagaccgctc cgccgaccgt
     3721 acccggtatt ccagacattc cggcaatgcc gcccaacccg cgagaaacgg atgcctccac
     3781 gagggaatcc cattcgtacc cggcgccaac agtcgcagac ccaccttcaa acgacagtct
     3841 gcggatggcg ttcaccagca caataccgtc cacaccgtca tc
//
LOCUS       DAOTNG010000295         3864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18900_length_3864_cov_5.221318, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000295 DAOTNG010000000
VERSION     DAOTNG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18900_length_3864_cov_5.221318"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="P5179_12075"
     CDS             complement(<1..424)
                     /locus_tag="P5179_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS95998.1"
                     /translation="MATTHEQLEANSLGADNSLRRYLEDIRTIPMLTREEEVELARRA
                     RAGDDAARARIVRANLRFVVSFAKRYQHRGLPLVDLVAEGNLGLVRAVDRFDETLGYK
                     FISYAVWWIRQAILQALSRQARPMRIPAGRTNRMLQLER"
     gene            complement(501..1721)
                     /gene="icd"
                     /locus_tag="P5179_12080"
     CDS             complement(501..1721)
                     /gene="icd"
                     /locus_tag="P5179_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRS95999.1"
                     /translation="MTAKTESPIRVQDGTLVVPDVPIIPYVEGDGTGPDIWRAAVRVF
                     DAAVGKAYGETRRITWREVFAGEKAFNSSGEWLPQATVDAFTHYLVGIKGPLTTPVGG
                     GIRSLNVALRQLLDLYVCLRPVKWFEGVPSPVKHPELVDMVIFRENTEDVYAGLELKE
                     GSTEANELADFCERRFGWTIRRDSGLGLKPISRTGTERLVRAAIDYAIKNGRKSVTLV
                     HKGNIQKFTEGAFRSWGYELAKREYRDHVVTWEECGGDVPAGKVLIKDAIADIFLQQV
                     LTRPVEFDVIATTNLNGDYISDALAAQVGGIGIAPGANINFETGHAIFEATHGTAPKY
                     AGQDKVNPGSVILSGEMMLRYIGWGEAADYVVKGLERTIAQRTVTYDFARLMEDAHEV
                     RCSEFGSAIIANMS"
     gene            2076..>3864
                     /locus_tag="P5179_12085"
     CDS             2076..>3864
                     /locus_tag="P5179_12085"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRS96000.1"
                     /translation="MSFPMPLPSDPDLIHAWTFSRLSAALDSGDAARSSLAASTWPEE
                     RRQRREQIGRVLVPDWEVPDPCVTITGSISRSDKRTGARWKIHKLRFQSLQDYWVTAL
                     LYVPETGRGPFPGMIVASGHLLEAKAGAEYHGISEELARQGMVVLAFDPVAQGERVQA
                     WDYVDNKFLVGWGTTEHDYDGQRALLCGWPLAQAFVWDGQRALDVLLSRPEVDPSRVG
                     VCGISGGGTQTTWLLAADDRFTAASPACFITGWREQFAARVGADPEQFPFPANGWGWD
                     QADVLLSFAPKPLLIVAATRDFFPIEGTRLTYQKLKDAYALIGAPGNVAMFEGDFDHA
                     YHPPLREATVRWFCGVFGIPYDGMGATEAPCDPDDLRVTPTGQLLTSGFTRTLRQWIA
                     QNAPDRSKSRVRGSALYHPDVWQARRRGLLSELLGIPSEAPQPSAIPLETNSWEGLTV
                     RRWLLTSEPGVEIPCALVFPPNEPCGLLCFLHEKGAATGWQLAGGPLSEAVDEGWAVL
                     SVDPRGVGAGVCRTQGNFYPDYHGRFGVESHITWTWTMLGRPFFAQRVFDVLQALRWA
                     RNMPDLAGRKSALVGVGSGALWALFASALD"
BASE COUNT          720 a         1206 c         1119 g          819 t
ORIGIN      
        1 gccgctccag ttggagcata cggtttgtgc ggccggcagg gatacgcatg ggcctcgcct
       61 gacgtgaaag tgcctgcagg atcgcctgac gtatccacca caccgcgtac gatatgaact
      121 tgtaccccag cgtttcgtca aaacggtcga ccgcgcgaac cagaccaagg ttcccttccg
      181 caacaagatc caccagcggc agccccctgt gctggtaccg tttggcgaaa ctgacaacga
      241 accggaggtt tgcgcgcacg atccgtgccc tggctgcgtc gtcgcccgcg cgcgccctcc
      301 tcgccagctc aacttcttcc tcgcgcgtca acatcggaat ggtacggatg tcttcgagat
      361 accgccgaag gctgttgtcc gcaccaagac tgtttgcctc aagttgttcg tgcgtcgttg
      421 ccatggaaac ccttccgctc gaaagagcgc atcgcgtcca cgttcgccgc acgaaacggg
      481 taccagtgat gcgacgcgct ctatgacatg ttggcgatta tcgccgaacc gaattctgag
      541 caccgcactt catgtgcgtc ttccattaac cgcgcgaaat catacgtgac cgtccgctgc
      601 gcaatcgttc gttccaggcc cttcactacg taatccgctg cttctcccca cccgatgtac
      661 cgaagcatca tctcgccgga aagaatgact gatcccggat taaccttgtc ctgccccgcg
      721 tatttgggag cagttccatg cgtggcctcg aaaatcgcat gaccggtctc aaaattgata
      781 ttcgcccccg gcgcgatgcc tattccgcca acctgcgcgg cgagagcgtc ggaaatgtag
      841 tcaccgttga ggtttgtggt tgcaatgacg tcaaattcca ccggcctcgt cagaacctgc
      901 tggagaaaga tgtccgcgat ggcgtctttg atgagaacct tgcccgcagg aacatcccca
      961 ccgcattcct cccacgtaac cacgtggtcc cggtattccc tcttcgccag ttcgtatccc
     1021 cacgaacgaa atgcgccctc ggtgaacttc tgaatgttgc ccttatggac aagcgtgacg
     1081 cttttccgcc cgtttttgat ggcatagtca attgccgcgc gtacgaggcg ttccgttccg
     1141 gttctgctga ttggcttcag ccccagcccc gaatcccgcc ggatggtcca tccgaaccgc
     1201 cgctcacaga aatcggccag ttcgttggcc tcagtggagc cttccttcag ttccaatcct
     1261 gcgtagacat cttcagtatt ctcgcggaat atcaccatat ccacgagttc cggatgcttc
     1321 accgggcttg gtactccttc aaaccacttc accggccgca ggcacacgta caggtccagc
     1381 agttgccgga gggcgacgtt aaggctgcga atgccgcctc cgactggcgt tgtcaacggg
     1441 cccttgatgc ccacgaggta gtgcgtgaag gcgtccacgg ttgcctgtgg cagccattcg
     1501 ccggaactgt tgaatgcctt ctctccggca aacacttccc tccacgtgat acgccgtgtc
     1561 tcaccatacg ccttcccgac ggcagcatcg aaaacccgga ccgccgcccg ccagatatcc
     1621 ggccccgtgc cgtcaccttc cacgtagggg ataatcggca cgtccgggac cacaagcgtt
     1681 ccgtcctgga ctcgtatcgg tgattcggtt tttgcggtca tcatgttccc tttgttgaag
     1741 aaatgagtaa tacaaacggc gtgccacacc gtgtaatgca cgcaaatcac ttataaacaa
     1801 ttatttaaat tcgtacgtat tgaaacatga gcaaaattcc ccagcaatca agaccggcat
     1861 tgggtaatat tacccaaatg gagatgtgat gtaaattact tgaacttgcg aatctcagag
     1921 gcgctcgttg ctctccggag cagtcattcc aacggggcta cgaaatgtac ggagacgggt
     1981 tgcccgtgtc tccccctccc cgttgcgctg gaaaccgctg ctggtgaact tctccacgtc
     2041 ctgccagaaa ccacccgtgt gcggaggatg cggccatgtc atttcccatg cctctccctt
     2101 ctgaccctga tctgatccat gcgtggactt tctcccggct ttcggctgca ctggatagtg
     2161 gcgacgcggc gcggagctct cttgccgcat cgacctggcc cgaggaacgc aggcagcgca
     2221 gagaacagat cggccgggtg ctggtgccgg actgggaagt gcccgacccg tgcgtcacca
     2281 tcaccgggtc catctcgcgc tcggacaaac gaaccggtgc gcggtggaag atccacaaac
     2341 tgcgcttcca gtcgttgcag gattactggg tcaccgcgtt gctgtacgtg ccggaaacag
     2401 ggcgcggccc gttcccggga atgatcgtcg cgagcggcca ccttctcgaa gccaaagcgg
     2461 gggctgagta ccacggcatc tccgaggaac tcgcccgcca ggggatggtg gtacttgcgt
     2521 ttgacccggt cgcacagggc gaacgggtgc aggcgtggga ctacgtggac aacaagtttc
     2581 ttgtaggctg gggaaccacc gagcacgatt acgacggcca gcgcgcgctt ctctgcgggt
     2641 ggccgcttgc tcaggcgttc gtctgggatg ggcaacgggc tctcgacgtt ctgttgagcc
     2701 ggccggaagt ggacccgtca cgcgtcggag tatgcgggat atcaggcgga ggcactcaga
     2761 cgacgtggct tctggcggcc gacgatcggt tcactgcggc ctcgcccgcg tgtttcatca
     2821 ccgggtggcg tgagcaattc gccgcgcggg tgggcgctga tcccgagcag tttccttttc
     2881 ctgcgaacgg ctggggatgg gatcaggcgg atgttctgct cagcttcgcg ccgaagccgc
     2941 tgctcatcgt tgcagcgacg cgggatttct ttcccatcga aggcacgcga ttgacgtatc
     3001 agaagctgaa agacgcctac gccctgattg gcgcgccggg caacgtagcg atgttcgaag
     3061 gggatttcga ccacgcgtac catccccctc tccgtgaggc tacggtgcgc tggttctgcg
     3121 gggtgttcgg aatcccgtac gacggcatgg gtgctaccga agccccgtgc gatcccgacg
     3181 accttcgagt gaccccgacc ggccagcttc tcaccagcgg cttcacacga acactgcgac
     3241 aatggatcgc gcagaacgcg cccgatcgct caaagtcccg ggtgagaggt tctgcgctgt
     3301 accacccgga cgtctggcag gctcgtcgcc gggggctttt gtccgaactt ctgggcattc
     3361 cgtccgaagc cccgcaaccg tccgccatac ctctggaaac gaactcctgg gaaggactta
     3421 ccgtacgccg gtggctgctc acgtcggaac cgggggtcga gattccctgc gcgcttgttt
     3481 tcccgccgaa cgagccgtgt ggcctgctgt gctttctcca cgaaaagggc gccgccaccg
     3541 ggtggcaact tgcgggaggg ccactttcag aggccgtaga cgagggctgg gccgtgctgt
     3601 cggttgatcc cagaggagtt ggtgccggag tctgtcgcac acagggaaat ttctaccccg
     3661 actatcacgg ccgtttcggg gtcgagtccc atatcacatg gacgtggacc atgctgggca
     3721 ggcctttttt cgcacagcgc gtgttcgatg tcctccaggc cttgaggtgg gcgcggaata
     3781 tgccggatct ggcgggcagg aagagcgcgt tggtaggagt tggctctggt gcgttgtggg
     3841 cccttttcgc ctcggccctc gacg
//
LOCUS       DAOTNG010000296         3860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_18924_length_3860_cov_5.379238, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000296 DAOTNG010000000
VERSION     DAOTNG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18924_length_3860_cov_5.379238"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5179_12090"
     CDS             complement(<1..730)
                     /locus_tag="P5179_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRS96001.1"
                     /translation="MAREYPLERTRNIGIMAHIDAGKTTTTERILLYTGRIHRAGEVH
                     DGAATMDWMEQEKERGITITSAATTCLWNEHRVNIIDTPGHVDFTAEVERSLRVLDGA
                     IGLFCAVGGVEPQSETVWRQANRYDVPRLCFVNKMDRTGADFYHVLDMMQDRLGGRFV
                     PLQIPMGAGDMFTGLIDLISMRAVTYNDESKGAVSQESEIPRDMVELANQYREKLLEA
                     VADFDDVVMAKFLDGNHDDITEAEI"
     gene            complement(829..1299)
                     /gene="rpsG"
                     /locus_tag="P5179_12095"
     CDS             complement(829..1299)
                     /gene="rpsG"
                     /locus_tag="P5179_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRS96002.1"
                     /translation="MARRRRVSKRELLPDPKYGSVVLAKFNNNMMVSGKKSTSEQILY
                     TALQMIGERTGQDEMSVFQKALDNIKPVIEVRPRRVGGSTYQIPVEVPSNRRQALAMR
                     WLIQYSRERSERTMAERLAAEIIAASNGEGNSVRKREDTHRMAEANKAFSHYHW"
     gene            complement(1315..1686)
                     /gene="rpsL"
                     /locus_tag="P5179_12100"
     CDS             complement(1315..1686)
                     /gene="rpsL"
                     /locus_tag="P5179_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRS96003.1"
                     /translation="MPTINQLIRHGRSQVTTSTKSPDLRRCPQRQGVCTQVKTTTPKK
                     PNSALRKIARVRLTTGREVTAYIPGEGHNLVEHSLVLIRGGRVTDLPGVRYHIVRGVQ
                     DAAGVANRHKSRSKYGTKRPK"
     gene            complement(1737..>3860)
                     /locus_tag="P5179_12105"
     CDS             complement(1737..>3860)
                     /locus_tag="P5179_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS96004.1"
                     /translation="EGERYNKIVEIWSHTSQRISKVMYENFATVENGFNPIYMMADSK
                     ARGNQEQIRQLAGMRGLMAKPQKKITGGIGEIIESPITANFREGLSVLEYFISTHGAR
                     KGLADTALKTAEAGYLTRRMVDVAQDVVVSELDCGTILGLDITALKEGEDVMVRLSDR
                     ILGRIALDDVVDPHTGEVVVEMGREITEALAAEIEERGIETVRIRSALTCESRTGICA
                     LCYGRNLATGKIAEIGEAVGVIAAQSIGEPGTQLTLRTFHTGGTAMQTGEQSRIDAPR
                     AGTVRFNNIQLAARDDAKIVISRGNGEIEILDEHGDPRARREVPYSAVLYVEDGQQVE
                     AGQALYESDPYNNTILTDSDGIVRFSDLISDVTFREEMSEAHIERVVVEQRNKALNPR
                     IEIVSSDGTKLRGYNIPVGARLIVQDGQQVRAGDQLVKIPRERSRTRDITGGLPRVAE
                     LFEARRPKEPAVVTEIDGVVRFGNVVRGSREVIVTSDVGDERHYLIPYGKYILVREGD
                     HIRAGERLSEGSANPHDILRILGPFRVQEYLLNEIQEVYRLAGVDINDKHIEIIVRQM
                     LQKVRVVEPGDSNFLEGDLVDKFRFMDENERIEEAGGQPATFEPVLLGITRASLMTES
                     FLSAASFQETTKVLTKAAVEGKTDYLRGLKENIVIGHVIPAGTGVRLHRNSTTEGGEL
                     EPLPERALDDEEEEAMLGTKVLDVG"
BASE COUNT          705 a         1239 c         1050 g          866 t
ORIGIN      
        1 gaatctcagc ctctgtgatg tcgtcatggt tcccatcgag gaacttcgcc atgacgacgt
       61 cgtcgaagtc agcgactgcc tcgaggagct tctcgcggta ctgattcgcc agctccacca
      121 tgtcccgcgg gatttcgctt tcctgcgata ccgcgccctt tgattcatcg ttgtaggtaa
      181 cagcgcgcat tgagatcagg tcgatcagcc cggtgaacat gtccccggcg cccatcggaa
      241 tctggagcgg aacaaaacgc ccacccagac gatcctgcat catatcgagg acgtgataga
      301 aatccgcccc cgtgcggtcc atcttgttga caaagcagag ccgcggcacg tcatatctgt
      361 ttgcctgccg ccacacggtc tccgactgcg gttccacacc accgacggca cagaagagcc
      421 cgatcgcgcc atccaagaca cgaaggctcc gctcaacctc ggccgtgaaa tccacgtggc
      481 cgggagtatc gataatgttg acgcgatgtt cgttccagag gcacgtcgtt gcggcggacg
      541 taatagtgat gccgcgttcc ttctcctgct ccatccagtc catcgtcgcc gcgccgtcgt
      601 gaacttcgcc ggcccggtgg atgcgtcccg tatagagaag aatccgttcc gtggtagtcg
      661 tcttgcccgc atcaatatgg gccatgatgc cgatattgcg cgtgcgttcc aatggatatt
      721 cgcgtgccat gtccagatct tgtcgtttct caccaagaca gacccgcctc tgcgtgggat
      781 gctatcctcc ccacagcaag aggcggttat atggtatggt gcttgggttt accagtgata
      841 gtgagaaaac gccttgttgg cttccgccat gcggtgcgtg tcctcacgct tgcgaaccga
      901 attgccttcc ccgttggacg cagcgataat ctctgccgcc agcctttctg ccattgtccg
      961 ttcgctgcgt tcacgcgagt actgaatcag ccatcgcatc gcaagcgctt gacgccgatt
     1021 ggaaggaacc tcaacgggaa tctggtaggt cgacccgcca acacgccgcg ggcgaacttc
     1081 tataaccggt ttgatgttgt cgagcgcctt ctgaaacacg gacatctcgt cctgccccgt
     1141 ccgctccccg atcatctgga gggcggtgta caggatctgc tccgacgtgc tctttttgcc
     1201 gctcaccatc atgttgttat tgaacttggc aaggacaacg ctgccgtact tggggtccgg
     1261 cagaagctct ctttttgata ccctgcgtct tcgcgccatt cttctacctc acccctactt
     1321 gggacgtttc gtgccgtact tggaccggct cttgtgtctg ttcgcaacac cggcggcatc
     1381 ctgaacgccg cgcacgatgt ggtagcgaac cccaggaaga tccgtcacac gcccgcctct
     1441 gatcagcacg agggagtgct ctaccaggtt gtgcccctcg ccggggatgt acgccgtcac
     1501 ctcgcgtcct gtcgtcaatc gcacacgggc gatcttccgc aaggcggagt tcggcttctt
     1561 cggcgtggtg gttttcacct gagtgcaaac cccctgacgt tgtgggcaac gacgcagatc
     1621 tggcgacttg gtcgacgtgg tgacctgcga gcgtccgtgt cggatgagct ggttgatcgt
     1681 cggcatgaac cgttcccttt tgtgaagcta cagagcaatt aaatgtatcg attgaactaa
     1741 ccgacgtcaa ggacttttgt ccccaacatc gcttcttctt cctcatcgtc caacgcgcgt
     1801 tccggtagcg gctcgagttc gccgccctcc gtcgtggagt tccgatggag ccggacaccc
     1861 gtgccggcag gaatcacgtg accgatgacg atgttctcct tcagcccacg caggtagtcc
     1921 gtcttgcctt cgacggccgc cttggtgagc accttcgtcg tctcctggaa gcttgcagcg
     1981 ctcaggaagc tctccgtcat gagcgatgcc cgggtaatcc cgagaagaac cggctcgaac
     2041 gtggcaggct gtccgcccgc ttcctcgatc cgttcgttct catccatgaa gcggaacttg
     2101 tccacaaggt cgccctcgag gaagttcgag tcgcccggct caaccaccct gaccttctgc
     2161 aacatctgac gcacgatgat ctcaatgtgt ttgtcattaa tgtccacgcc ggccagacga
     2221 tacacctcct ggatttcgtt cagcaggtat tcctgcacgc ggaacggccc cagaatgcgc
     2281 aggatgtcgt gcgggttcgc tgatccctcg gaaagccgct cccctgcgcg aatatggtca
     2341 ccctcgcgca ccaggatgta cttgccgtac ggaatcaggt aatggcgctc gtccccgaca
     2401 tcgctcgtca cgatgacttc gcgcgaaccg cgcaccacgt tgccaaaccg cacaacgccg
     2461 tcaatttccg tgacaacggc cggttctttc gggcgccgcg cttcgaacaa ctcggccacg
     2521 cgaggcaatc cgccggtgat gtcacgcgtt cgagaacgct cgcgcggaat cttcaccagt
     2581 tgatcgccgg cccgtacctg ttgcccgtcc tgcacgatca ggcgggcacc gaccgggata
     2641 ttgtaccccc ggagcttcgt cccgtccgaa gagacaatct cgatacgtgg gttcagcgcc
     2701 ttgttccgct gttcgacgac gacgcgctcg atatgcgcct cggacatctc ttcgcggaac
     2761 gtcacgtcgc tgataagatc gctgaaccgg acaataccgt ccgagtcggt cagaatcgtg
     2821 ttgttgtacg ggtcgctctc gtacagcgcc tgccccgctt ccacctgctg accgtcttcc
     2881 acatacagca ccgcagaata ggggacttct ctccgcgcac gcggatcgcc gtgctcatca
     2941 aggatttcga tctcaccgtt tccccggctg atgacgattt tcgcgtcatc ccgcgcggcg
     3001 agctggatgt tgttgaaacg gacggtaccg gcgcgcggag cgtcaattct cgactgttcc
     3061 ccggtctgca tggccgttcc accggtgtgg aaggtccgca acgtaagctg cgtgcccggt
     3121 tcgccgatgg attgcgccgc gatcacgccg accgcttctc cgatctcggc gatcttgccg
     3181 gtcgcaaggt tccgaccgta acagagcgcg cagatgcccg tgcgggattc gcaggtgagg
     3241 gcggaacgga tgcgtaccgt ctcgataccg cgctcttcga tttccgcggc aagcgcctcg
     3301 gtaatctcgc gccccatctc gactacgact tcaccggtat ggggatctac gacatcgtcc
     3361 agcgcaatac ggccaagaat gcggtccgaa agccgcacca tcacgtcttc gccttctttg
     3421 agcgcggtga tgtccagccc gagaatcgtg ccgcaatcca gctcagagac aaccacgtcc
     3481 tgcgcaacgt caaccattcg gcgcgtaaga taacccgctt ctgcggtctt caacgccgta
     3541 tcggcaagcc ctttccgcgc gccgtgcgtg gagatgaagt actccagcac cgaaagccct
     3601 tcgcggaaat tcgccgtgat gggcgattcg atgatttcgc cgatacctcc cgtgatcttc
     3661 ttctggggct tcgccatcag cccgcgcata ccggcgagct ggcgtatctg ttcctggttt
     3721 ccacgggctt ttgaatccgc catcatgtag atgggattga acccgttttc cacagtcgcg
     3781 aagttctcgt acatcacctt ggagatgcgc tgcgacgtgt gcgaccagat ttccacgatc
     3841 ttgttgtagc gctcaccttc
//
LOCUS       DAOTNG010000297         3844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19013_length_3844_cov_4.059382, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000297 DAOTNG010000000
VERSION     DAOTNG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19013_length_3844_cov_4.059382"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5179_12110"
     CDS             complement(<1..701)
                     /locus_tag="P5179_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96005.1"
                     /translation="MIAKKAARRALSVFCSLFCATNALAADIVPRPIVGEIAGAVRIP
                     ARVAIVMSAEERARVYAWAGYRLNIGTPVENTLLQALAQVFDSVCVVPALPAPSDVGE
                     EAALFVEPRVDRFQLSQSLFSLSQVRCEMAVQYVAVGRRRGVLVEIAQYRINVPRMTV
                     WSEHFGGSFETAIKEAASRTTAAAARGLVEAMSVDDTLARFVREHFPAPSPTGASPVF
                     SMTSFDPNRLSIAVL"
     gene            complement(1164..2369)
                     /locus_tag="P5179_12115"
     CDS             complement(1164..2369)
                     /locus_tag="P5179_12115"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRS96006.1"
                     /translation="MISADIAPKLATETGSKIVLMIMDGLGGFPNETGKTELETAHTP
                     NLDTLAAEGATGLIDTLGRGIPPGSGLAHLALFGYDAFVHDIGRGAIAANGIGLTLKP
                     NQVAARLNFCTQQNGLITDRRAGRIPTEKCAELCRVLNAKVNVPGVRIEAYPVMDYRA
                     VVVFEGDGLSINIGDTDPGATGVPAKPVKATGAGAEKMVGVVEAFIAQAKEALAALHP
                     ANMILLRGFAVQPDFPSMQACYKLKPACIATYPDYRGVAKMIGMDILKTGMEIAEEVD
                     TLEQDWKSDHTFYFFHVKKTDSYGEDGNFDGRVHKIEEVDALIPRIMALKPDVFAVTG
                     DHSTPAVFKAHTWHPVPVLLWGKTVRRDSTTSFGERAAICGGMGRFEGSALMLELLAH
                     AGKLKKMGA"
     gene            2608..>3844
                     /locus_tag="P5179_12120"
     CDS             2608..>3844
                     /locus_tag="P5179_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS96007.1"
                     /translation="MKRSCFPGTHVTAALIFLGSVASAVTFTPKLTLPPVQYGAQSWA
                     IPDSSGTYAGKSILALSGQTPGGTLKTYIVWIESQSGVPGVAKIDSSSIPGIVFGQVD
                     WADYNRDGLLDLAISGRTVSATDTLAQDITMVYRQYSDGSFRPEAAFPTGYGMDSTIV
                     RWVDYDNDGDMDLFVSGRKRILSLNPSTGKKEVTGESRLHAFRNDSPTPQVTVFVRDT
                     GVRSRVDQIIPVHDGDADWADIDGNGTPDLAITGKSATFLQGNRVATEIVTEVYLNNP
                     AGVFTRNVLTSLPPRYGGGIAWGDMNEDGLLDIALSGYAKPADGGALTLEVWLHLGEG
                     SFRKQAIQLDQRDAVAGRLAWMDVDNDGRFDLVAMGDSSATVSGIRLYRNNPDLTLSR
                     ISTSLPALGDGAFSLGHLDT"
BASE COUNT          743 a         1182 c         1168 g          751 t
ORIGIN      
        1 tcgagaaccg cgatgctgag gcggtttggg tcgaacgacg tcatggagaa gaccggagaa
       61 gcaccagtgg gggatggagc agggaaatgc tcacgcacga aacgcgcgag ggtgtcgtct
      121 acggacatcg cctcaaccaa accccgagcg gccgctgcgg ttgtgcggga ggcagcttcc
      181 ttaattgccg tttcaaagct tcccccgaag tgctcgctcc acacagtcat tcttggcaca
      241 ttgattcggt attgcgcaat ttcgacgaga acacctcgcc gtctaccaac ggcgacgtat
      301 tgcaccgcca tctcgcaccg gacctggctg agcgagaaca aactctgtga tagttggaaa
      361 cgatctaccc ttggttctac gaacagcgcg gcttcttctc caacgtcgct tggcgcgggc
      421 aatgcaggca ccacacacac tgaatcgaag acctgcgcca acgcttggag cagcgtgttt
      481 tcgacgggag taccgatgtt cagcctgtaa cctgcccatg catagacacg cgccctttcc
      541 tcggcgctca tgacgattgc cacgcgcgcg gggatgcgaa cggcacccgc gatctcccct
      601 acgatcggcc gcgggacaat gtcggcggcc aaggcatttg tggcacaaaa gagactgcag
      661 aacacggaaa gagcacgacg agcggctttc ttcgcaatca tccagacttc cccacgatgg
      721 atgcaagtag gctttgtcgt tgcgccaccc gacactaaat aagatagaac cgagccgggt
      781 cttgtacacc cctttgggaa tggtgctctt ctgactactg gattcccgcc ttcgcgggaa
      841 tgacgactga gcgtaggatg cgcgtgcccg ggaacgacgt cgaaaccacg gtaccgtccg
      901 cgccgccatt tcctgatgca acaggtaccc ggctttttcc ctcccgtcgg cgtttgaaag
      961 acgtgatttc cgcgtgctcg gcggagcacg aagcgcaggc gggaatccag cgggggcttc
     1021 gacgggctgc ccgccagagc ggcctgctgc ccgcgagggc ggcaggccgc ccacctaagg
     1081 cggcacgccc agccgccacg cgcgccgcgc acccctgcga caaaaaaagg gcgcgacgaa
     1141 gcgcgcccct ccatttcggt tccctacgca cccattttct tcagtttgcc tgcgtgcgcg
     1201 aggagttcca gcatcagtgc ggaaccttca aaccgcccca tcccgccgca gattgcagcc
     1261 cgttcgccga atgaggtggt ggagtcgcgc cggacggtct tgccccagag gagcaccggc
     1321 accggatgcc aggtgtgcgc cttgaacacc gcgggggtgg agtgatcgcc cgtaacggcg
     1381 aacacatccg gcttcagcgc cataatgcgc ggaatgagcg cgtccacttc ctcgattttg
     1441 tgcacgcggc cgtcgaagtt gccgtcctcg ccgtagctgt cggtcttttt cacgtggaag
     1501 aagtagaagg tgtggtcgct tttccagtcc tgctccagtg tgtccacctc ttcggcgatt
     1561 tccatccccg ttttcaggat atccatgccg atcattttcg ccacgccgcg gtaatcgggg
     1621 tacgtggcga tgcaggcggg cttgagtttg taacaggcct gcatggaggg aaaatcgggc
     1681 tgcacggcaa acccgcgaag cagaatcatg ttcgcggggt gcagcgccgc cagcgcttcc
     1741 ttcgcctgcg cgataaatgc ctcaacgaca cccaccatct tttccgcgcc ggcgccggtg
     1801 gctttgacag gtttcgccgg aacgcccgtg gcgccggggt cggtgtcccc gatgttgatg
     1861 gataacccgt cgccttcgaa caccactact gcgcgataat ccatcaccgg atacgcttcg
     1921 atcctgacac ccggcacatt caccttcgcg ttcaggaccc tgcacagctc cgcgcatttt
     1981 tccgtcggaa tcctgcctgc ccgccggtcg gtgatgaggc cgttctgttg cgtgcagaag
     2041 ttgaggcgcg cggcgacctg attcggcttg agcgtgagac cgatgccgtt ggcggcgatt
     2101 gcgccgcggc cgatgtcatg tacgaaagcg tcgtagccga acagcgcgag gtgtgccagc
     2161 ccgctgccgg gaggaatgcc gcgccccagc gtgtcgatga ggccggttgc gccctcggcg
     2221 gcaagggtat cgaggttcgg agtatgcgcc gtttccagtt cggttttccc ggtttcgttc
     2281 gggaacccgc caagtccgtc catgatcata aggacgatct ttgaacccgt ttcggtggcg
     2341 agtttcgggg ctatgtctgc gctaatcatg gcattcctcg catcaggtgt gaaaatccca
     2401 ccagaaatgc gcggccaatt ccctcggcca cgcggctctg agaaaactac gcagtccgtg
     2461 tgaacgcgga caaggtgcgg tgcggcgcaa ggctcgcttg acgtaggaac aaaaatgcgt
     2521 tatttatggc gtgactgaga ccgtgaactg ctgaacttca gagcagttac ggcgtagcgt
     2581 acaacctcgc ttccctgatg gtacgccatg aagaggtcct gctttccggg cacccatgtc
     2641 actgctgcat tgattttcct cggttctgtt gcgagcgcgg tgacatttac gccgaaactc
     2701 accctgcccc ccgtgcagta cggggcgcag tcgtgggcga ttcccgacag ctccggcacc
     2761 tacgccggca agagcattct cgccctctcc ggccagacgc ccggcggaac actgaagacg
     2821 tacatcgtct ggattgagtc gcagtcaggg gttccgggcg tggcgaaaat cgactcatcc
     2881 agcatacctg gtatcgtgtt cgggcaggtg gactgggcgg attacaatcg ggatggtctg
     2941 ctggacctcg ccatcagcgg gcgaacggtg agcgcgacgg ataccctcgc ccaggacatc
     3001 acgatggtgt accgccaata ctccgacgga tcattccgtc cggaggcggc cttccccacc
     3061 gggtacggga tggactccac gattgtgcgg tgggtggatt atgacaacga cggagacatg
     3121 gatcttttcg tcagcggccg aaaacgaatc ctttcgctga acccgtccac gggtaagaaa
     3181 gaagtaacgg gggaaagccg tctgcatgca tttcgtaatg actctcctac gccacaggtg
     3241 accgtgtttg tgcgggatac cggcgtgcgc agccgggtgg accagatcat cccggtgcac
     3301 gacggtgatg ccgactgggc cgacatagac ggaaacggca cgcccgatct cgcaatcacg
     3361 ggaaaaagcg ccacgttcct tcaggggaat cgcgtggcaa cggaaatcgt gaccgaggtc
     3421 tacctcaaca acccggcagg cgtgttcacg cggaatgtgc tgacttccct tcccccgcgg
     3481 tacggaggcg gcatcgcgtg gggcgacatg aatgaggacg ggctgctgga tatcgccctc
     3541 tccgggtacg cgaaaccggc cgatggcggc gctctgacgc tggaagtgtg gctccacctc
     3601 ggcgaagggt ccttccgcaa acaggcaatt cagctcgatc agcgcgatgc ggtggcgggc
     3661 agactggcgt ggatggacgt ggacaacgac ggccgctttg atctcgtcgc gatgggcgat
     3721 tccagcgcca cggtgagcgg catccgtttg taccggaaca accctgatct cacgctttcc
     3781 cgtatatcca cctcgttacc ggcgcttggc gacggcgcgt tctccctcgg ccatctggac
     3841 actg
//
LOCUS       DAOTNG010000298         3819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19156_length_3819_cov_4.675345, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000298 DAOTNG010000000
VERSION     DAOTNG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19156_length_3819_cov_4.675345"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="P5179_12125"
     CDS             <1..238
                     /locus_tag="P5179_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96008.1"
                     /translation="AGQRVYTVPVSVARVGILILVLAGCLVLAPTTTTRFGTTLQGIA
                     LRALLTVLMWSACLAAVGPGDVLRFAATMVGKRT"
     gene            235..1707
                     /locus_tag="P5179_12130"
     CDS             235..1707
                     /locus_tag="P5179_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96009.1"
                     /translation="MNVAGRLSSGADSFHQLAIAGATSAALVGAVFMLPPEVSAFLFY
                     VSAGAIILCIWIVRSAPREDVTGLLVGALCALILRAALAWWVFDHPERYSDTRLYWTL
                     GNRIASLWEPGRILPVEMVKATGTSSWGFFFWSGLHISILRTPLLLVAANGIAGTAVG
                     VMAYQIGRHVWSREVGRRAAWLVWISTSQLIVDAHLLRDSGAILFGAGVVYGSLLLAD
                     RLSLRGLLWYGISFFLLMQMRSYIAMVVAPASLLGVLIGARGRRLSLLLIYLVAGTGA
                     ALFLASTDFVRILTALGRKNVIADMLYLTQVGLSGIRPSASYFSGVHLSTPREMIAFL
                     PFGTMRELLSPLPWMPIEMDRWFIPEGLLRWTLIPLFVLGVVEALRKNGRVVATVALV
                     MAAEMVIYSIIELGGNPRHNAQFFPYVYIFAMIGLSVARKHARALWLMYGALSLGIWT
                     FAVTVSFLRLIFFPLGAAMVVWLSLVAYVNRKTLFEERVE"
     gene            1704..2852
                     /locus_tag="P5179_12135"
     CDS             1704..2852
                     /locus_tag="P5179_12135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS96010.1"
                     /translation="MTRRVLHLLGSLGAGGEERQTLTLVKGLCGSRWEPIVCGMRGGA
                     LAEEFARHARTIILEKRRKVDLAALARIRAIMDEVRPAVLHCHLSTANTWGRIAALMR
                     PEKRPAVVVSEGGIETWKTVVHLAIDRALLGVTDAVVGNAEAIAHYLIDHDRIPARIV
                     RVIPNGIDLARVQAKLHATEEERVSRRKELGLTSDHFVVGHVGRSCAVKGLDTLAAVI
                     EQMVRLNPDFRLIRAGQPPLPDEIEPAEAFRRDVEARGVADHVIWHPYTPDISSVLMI
                     LDALVQTSLSEGLPNAVLEAMAMGVPVVATAAGGTSEIVRHRETGWLTEIGNARGLCE
                     GLEHVRNDPEESRLWACRARTMVEHDFSLARMVENFCDLYDRVAGLHP"
     gene            2997..>3819
                     /gene="thrB"
                     /locus_tag="P5179_12140"
     CDS             2997..>3819
                     /gene="thrB"
                     /locus_tag="P5179_12140"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRS96011.1"
                     /translation="MGKRPQGWSIRVPATTANLGPGFDCFGLALQRYLNLFVTVRGEK
                     GAWHITLHGEGAGILPRDRTNLIYRALVLGSEVDAESLPGISIRMENAIPLARGQGSS
                     AAAIVAGMAAAQLLTVGKIDPDQLINRATLVEGHSDNVAAAVKGGLVLTRESPSGVAI
                     RPIPTRDGALFVSAVPEFELTTHTSRSVLPQQVPLGDAVFNLRAAAWMASAWAAGDWE
                     AVGNAMEDRLHQPYRAPLIPGFDDVCRAARAAGALGACLSGSGPAIVALALHSSDA"
BASE COUNT          656 a         1123 c         1193 g          847 t
ORIGIN      
        1 cgcagggcag cgcgtgtaca ctgtgccggt gtctgtcgct cgcgtgggta tcctcattct
       61 cgtactggcg gggtgcctgg tgctcgcgcc gacaacaacg acaaggtttg gaacgaccct
      121 tcagggaatt gcgctcaggg cactcctcac tgtgctgatg tggtcggcct gcctcgccgc
      181 ggtgggcccc ggggacgtgt tgaggtttgc cgcaacaatg gtgggaaaga gaacatgaac
      241 gtcgcaggcc gcttgtccag cggagcagac tcgttccacc agttggcaat cgccggtgca
      301 acgtccgccg cactcgtcgg cgcggtgttc atgttgcctc cggaagtatc cgcgtttctt
      361 ttttacgtgt ctgcgggcgc aatcattctc tgtatctgga tcgttcgttc cgctccccgc
      421 gaagacgtga caggattact cgtcggagcg ctctgtgcgc tcattctgcg cgccgctctt
      481 gcctggtggg tgtttgacca tcccgagcgc tactctgaca cccgcctcta ctggacgctg
      541 gggaaccgga tcgcgtctct ttgggaacct ggccgcatcc ttcccgtaga aatggtgaaa
      601 gcaaccggga caagctcctg ggggttcttc ttctggtccg gcctgcatat ttccatcctc
      661 cggacccccc tcctgctggt cgccgcgaac gggatcgcag gaaccgcggt cggtgtgatg
      721 gcctatcaaa ttggtcgtca cgtctggtcg cgcgaggttg ggcggcgtgc ggcatggctt
      781 gtctggatat caacctctca actcatcgtc gacgcccatc ttcttcgaga ttcgggggca
      841 atcctgttcg gcgccggcgt ggtgtacggt tcactgcttc tggcggaccg cttgagcctg
      901 cgcggattgc tctggtacgg gatcagcttc ttcctgctga tgcagatgcg cagttacatc
      961 gcaatggtcg tggcgccagc ttcgctgctc ggggtcctca ttggtgcccg ggggcgaagg
     1021 ctctcccttc ttttgatcta cctcgtggcg gggacaggcg cggctttgtt cctcgcctcg
     1081 acggacttcg tacgaatcct taccgctctc ggcaggaaaa acgtcatcgc cgacatgctc
     1141 tacctcacgc aggtagggct gagcggcatc cgtccatctg cgtcgtactt ctcgggtgtg
     1201 cacctgtcaa cgccgcggga aatgattgcg tttcttccgt ttggaaccat gcgggagttg
     1261 ctgtcacctc tcccatggat gccgatcgag atggacagat ggtttatccc ggaaggcctg
     1321 ctccggtgga cgttgattcc gttatttgtt ctcggggttg tggaagctct caggaagaac
     1381 ggccgtgttg tcgcaaccgt ggccctggtg atggccgccg agatggtgat ctactcgatc
     1441 atcgagctcg gaggcaaccc gaggcacaat gcacagttct tcccgtacgt gtacatcttt
     1501 gcgatgatcg gcttgtcggt cgcgaggaag cacgcgcgcg cactctggct catgtacggc
     1561 gcgctcagtc tcggcatctg gacgttcgcc gtcacggttt ccttcctgcg gttgatcttt
     1621 ttcccccttg gcgctgcgat ggttgtctgg ttgagcctcg tggcgtatgt caacagaaag
     1681 actctctttg aggaacgcgt ggaatgaccc gtcgagtgtt gcatcttctt ggcagccttg
     1741 gcgcgggcgg ggaggaacgc cagacgttga cccttgtcaa ggggttgtgc gggagtcggt
     1801 gggagccaat cgtgtgcggc atgcgcggag gagcgcttgc ggaggagttc gcgcggcacg
     1861 cacgaacaat catactggag aagagacgga aagttgacct tgcggcgctc gcgcgtattc
     1921 gggcaattat ggacgaagtt cggcccgccg tgttgcactg ccacctctcc accgccaata
     1981 cctggggcag gatcgcggcg ctgatgagac cagaaaaacg ccctgctgtc gttgtctctg
     2041 aaggaggcat cgaaacctgg aaaaccgtag ttcacctcgc gattgacagg gcgctcctcg
     2101 gcgtgacgga tgcggtggtc ggaaatgccg aagccatcgc ccactacctc atcgatcatg
     2161 accgaatacc ggcacgtatc gtccgcgtga ttccgaatgg aattgatctg gcgcgggtcc
     2221 aggcgaaact ccacgcaacg gaagaagaac gcgtctcgcg caggaaggaa ctgggcctca
     2281 cttcggatca tttcgttgtc ggtcatgtcg gccgttcctg tgcggtgaaa ggccttgaca
     2341 cgctggcggc ggtgatcgag caaatggttc gcctgaaccc cgatttccgc cttatacgcg
     2401 cgggacagcc acctttgcct gacgaaatcg aacctgcgga agcgttccgt cgtgacgtcg
     2461 aggcgcgggg cgtcgcagac cacgtaatct ggcatccgta cacacccgac atctcttccg
     2521 tactcatgat ccttgacgcc ctggttcaga catccctcag cgaggggctg cccaacgctg
     2581 ttctggaagc aatggcgatg ggtgttcctg tagtggcaac ggctgcgggc ggtacctcgg
     2641 aaatcgttcg gcaccgggag acggggtggc tcacggaaat cgggaatgcc agagggcttt
     2701 gcgagggtct tgaacacgta cgaaatgacc ccgaagagag ccggttgtgg gcgtgtcgag
     2761 cgcgaacaat ggtggaacac gacttctcgc tcgcacgcat ggttgagaat ttttgcgatc
     2821 tgtacgatcg cgtcgccggg ctccacccgt gaccctcgcg tgcaacgcag cgtgtatcca
     2881 tcgcattccc gttgacggcg caccgccgcg gaacgatcat ttttacgggg cgaacccgca
     2941 tcgggcttac ttcacgctga tacccacccg gcatcttcac cagtacgagt tccgcaatgg
     3001 gaaaaagacc acagggatgg agcatacgcg ttccggctac gacggcaaac cttggcccgg
     3061 gttttgattg tttcggactc gcacttcagc ggtatctgaa tcttttcgtc acggttcgcg
     3121 gggaaaaggg cgcctggcac atcacgttgc atggcgaggg ggccggtatt ctgccccggg
     3181 accgcaccaa cctgatatac cgcgcactcg tactcgggtc tgaggttgat gcggagtcgc
     3241 ttcccggtat ctccatccgc atggaaaacg ccattcctct cgcgcgcggt cagggttcca
     3301 gtgccgcggc gattgttgcc ggcatggcgg cggcgcaatt gttgaccgtg ggaaagatcg
     3361 atccggacca gctcatcaat cgagccaccc ttgtcgaggg gcattcagat aatgtggctg
     3421 ccgccgtaaa gggtgggctt gtccttacca gggaaagccc ttcaggagtt gcaatccgtc
     3481 cgattcccac ccgcgacggc gcgttatttg tttcagcggt cccggaattc gagctgacca
     3541 ctcacacgtc gcgttcggtt cttcctcagc aggtgccttt gggagatgcc gttttcaacc
     3601 tgcgtgccgc cgcctggatg gcttccgcgt gggcagccgg cgactgggag gctgtcggca
     3661 acgcgatgga agacagattg caccagccgt accgagcgcc gctgatcccg gggttcgacg
     3721 acgtatgcag ggctgctcga gccgctggag ccctgggtgc atgcttgtcg ggttcgggcc
     3781 ccgcgatagt cgcgctggcg ctccactcat cggacgccg
//
LOCUS       DAOTNG010000299         3817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19171_length_3817_cov_5.936470, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000299 DAOTNG010000000
VERSION     DAOTNG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19171_length_3817_cov_5.936470"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="P5179_12145"
     CDS             <1..1873
                     /locus_tag="P5179_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS96012.1"
                     /translation="SSPSPAAEEDSTISVWVFLTDHGESRGVLNRPSAPVSDRARARM
                     ATRGSRTEDVLAVYAPYVERVSSLVNRVRTVSRWLNAVSADATPAQIESLRALPEVRA
                     VSPVRTYRHELPDIDATQARMAPGLADTVQQRYGASFWQYGRVGIPELHALGLTGRGV
                     MICVIDAGFTRFDHPAFAHVKVVQTKDFITGSTSLTPDSHGAQVLSVIAGNEAGMLVG
                     PAYGAEYMLARTEARAVEVRAEEDFWIAAVEWADSAGVDIITSSLGYNTFDDPSENHS
                     LAELDGSSLITRAADRAVARGIVVVASAGNERGASGAPEWQGRITLPADGFGVLAVGS
                     LTAADQIAASSSTGPTADGRIKPDLVAPGDNVLALDVAMTSGYARVSGTSFAAPIVAG
                     AAALLLEKNPLWTPADVRRALLWSAEDILAPGPDVFSGWGVMNANLAVDEVVHSGIVG
                     RVYSTVAYVGGQAKDVPVAGVRVELRNGSGVLVGETTTDVRGRFSFSGQPDGAYRVRL
                     SHPGFDETQSDVTIPQTVQLRFTLLPASSAGAQAYRLFPNPATPASGVTISGAAHPDL
                     IAKFYDISGTLVRTLGPQQMYWDLTSQGGRQVAGGVYFCRIEVAGSEVWNGKIAVVR"
     gene            1899..2402
                     /locus_tag="P5179_12150"
     CDS             1899..2402
                     /locus_tag="P5179_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRS96013.1"
                     /translation="MERLWAPWRMKYILGEAGRSEGCVFCEAAHTTDDAAHLVVGRMS
                     CTMTIMNLFPYTNGHMMVIPYRHVSGLSDLTSEERLEIMEGAATAVDVLRETFHCDGN
                     NLGFNLGRPAGAGIDAHLHMHVVPRWNGDTNYMAVLDDTRVISEALEETYRRLRAAYR
                     ERGLAPA"
     gene            2466..2873
                     /locus_tag="P5179_12155"
     CDS             2466..2873
                     /locus_tag="P5179_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96014.1"
                     /translation="MSWYVRIALYATILLGTAFGLNRLRGRIPQAFGLTPIRVEVLSL
                     ERFRDRDLRGGKENTFGVVARITGRKSPSASVSLRQFVLKTESGQLVRPYASSLQFDH
                     LGNLAIAHADTLTGVFLFSVPSTEKPSEFWWNP"
     gene            2870..>3817
                     /locus_tag="P5179_12160"
     CDS             2870..>3817
                     /locus_tag="P5179_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRS96015.1"
                     /translation="MNAHEPFPVSVGDVIELDVHDQPYGDAALGRFSRLAVFVPGAVA
                     GDRVLARVTEVHTRYARADLISVVTSSPDRVPAECPLAGRCGGCQWQTTAMARQHAAK
                     HGAVVETLRRVGKWEEPPVRDILPSGEPWRYRNKARYAVVTGAAGTGIGYFGTRTHDL
                     VSISRCPLNMERLDALLDAAHDLLINDPRFDDLRTTTNSLTGRTSAATGETVLRVAAK
                     AASDWGAFAEALRQRIPFVSGVVVAGHPRRGDRVVWGSGSLTERVRGNEYRVSAASFF
                     QVNPFAVGNLVDTVADAAGLQGSENLVDAYGGVGLFSLVI"
BASE COUNT          684 a         1107 c         1261 g          765 t
ORIGIN      
        1 ttcctcccct tcacccgcgg cagaagaaga ttccaccatt tccgtgtggg tttttctcac
       61 cgaccacggt gaatcgcgcg gcgtgctcaa ccgtccttcg gcgcctgttt ccgaccgcgc
      121 aagagcacga atggccactc gcggttcgcg gacggaagac gttctggctg tgtatgcccc
      181 gtatgtcgaa cgggtctcat cgctcgtcaa ccgcgtgcgc accgtctccc gctggctgaa
      241 tgcggtaagc gcggacgcaa caccggcaca gattgagagc cttcgcgcgc tgcccgaggt
      301 acgcgctgtc agcccggtac gtacgtaccg gcacgagctg cccgacatcg acgccacgca
      361 ggcgcgcatg gcacccgggc ttgcggacac ggttcagcaa cggtacggag ccagtttctg
      421 gcagtacggc cgagtcggga ttccggagct gcatgcgctc ggccttaccg gaaggggggt
      481 catgatttgc gtgattgacg ccggatttac ccggtttgat caccccgcgt ttgctcacgt
      541 gaaagtcgtc cagacgaagg acttcatcac cggttctacc agtttgacgc ccgattcaca
      601 cggggcgcag gttctgtcgg tgattgccgg gaacgaagcg ggaatgctgg tcggtccggc
      661 gtacggtgcg gagtacatgc ttgcccgtac ggaagcccga gcggtggaag ttcgcgccga
      721 agaggatttc tggatcgcgg cagtggaatg ggcggactcc gccggcgtgg acatcatcac
      781 gagcagcctc gggtacaaca cgttcgacga tccgtccgag aaccacagtc tcgcggaact
      841 ggacggctct tcgttgatca cccgggccgc tgatcgtgct gtggcgcgcg ggattgtcgt
      901 cgtggcttcg gcgggaaatg aacgcggtgc gagcggggcg ccggaatggc aggggcggat
      961 tacccttccc gcggacggtt tcggggttct ggctgtcggt tccctcaccg cggccgacca
     1021 gatcgcggca tcgagttcaa ccggaccaac ggccgacgga agaatcaaac cggatctggt
     1081 ggcaccgggt gacaacgtgc tggcgctcga cgttgcgatg acgtcgggct atgcgcgcgt
     1141 ttcgggcaca tccttcgcgg caccgatcgt cgccggcgcg gcggcgttgc tgctggagaa
     1201 gaatccgctc tggacgcccg ctgacgtgcg ccgcgcgctc ctctggtcgg cggaagacat
     1261 tcttgcgccg gggccggatg ttttttccgg gtggggagtg atgaatgcca acctggcagt
     1321 agatgaagtc gttcactcgg gtatcgtagg gagggtatat tcgaccgtcg cgtacgtcgg
     1381 tgggcaggcg aaggacgtac cggttgccgg tgtgcgtgtg gagctgcgaa acgggagtgg
     1441 cgttctggtg ggtgagacaa ccacggacgt gcgggggagg ttcagtttca gcggtcagcc
     1501 tgatggcgcg tacagggttc gtctctccca tccagggttc gacgagactc aatcggacgt
     1561 cacaatccca caaaccgtgc agttacgctt cacgcttctc cctgcctcgt cggccggcgc
     1621 ccaggcgtac cgtttgttcc cgaatcccgc gacgcccgcg agcggcgtta cgatttccgg
     1681 cgcggcccac cctgacctca tcgcgaaatt ctacgatatt tccggtacgc tggtgcgaac
     1741 gctcgggccc caacagatgt actgggacct cacttcgcag ggcggaagac aagtagcggg
     1801 aggcgtgtat ttctgcagga tagaagtggc aggttctgag gtgtggaacg ggaaaatcgc
     1861 tgtcgtccgg taatgcggcg ggaatgagag gtaaacctat ggaacgcctc tgggctcctt
     1921 ggcgaatgaa atacatcctt ggcgaagcgg gcaggtcgga aggatgtgtt ttctgcgagg
     1981 ccgcgcacac caccgacgac gcggcgcatc ttgttgtggg acggatgagc tgcacgatga
     2041 ctatcatgaa ccttttcccg tacacaaacg gtcacatgat ggtgattccg tacaggcacg
     2101 tctccggcct ttccgatctc acgagcgagg agcggctcga aatcatggaa ggggcggcaa
     2161 ccgcggtgga cgtacttcgg gagaccttcc actgcgatgg caacaacctt ggattcaacc
     2221 ttggcaggcc tgcgggggcg ggcatcgacg cacaccttca catgcacgtg gttcctcgct
     2281 ggaacggtga cacgaactac atggcggttc ttgacgacac gagggtgatt tcggaggctc
     2341 tggaggagac ctacagacgg ctcagagctg cataccgcga gcgtggactt gcgcctgcat
     2401 gacgccggga cagtgaaccg acgatgggga tgtggacggg ttttcacttc ggaagcacac
     2461 ctcgcgtgtc ctggtatgtg cggatcgccc tctatgcgac gattcttctg ggaaccgcat
     2521 tcgggttgaa ccgtctgcgt ggccggattc ctcaagcgtt tggcctcacg cctattcggg
     2581 tggaagtgct ctcgctggaa cgatttcggg accgagacct ccgcggaggc aaggagaaca
     2641 cgttcggagt cgtcgcgaga atcaccggaa gaaagagccc ttccgccagc gtatccctcc
     2701 gacaattcgt gctcaagacc gagagcgggc agctggtccg cccgtatgcc agttccctgc
     2761 agtttgacca tctgggtaac ctggcgattg cgcatgcgga cacgctcacc ggcgtcttcc
     2821 tgttttccgt tccctccact gagaaaccgt ctgagttctg gtggaacccg tgaacgcaca
     2881 cgaaccgttc cccgtgtcag tcggggatgt cattgagttg gacgtccacg accaaccgta
     2941 tggcgacgcg gccctcgggc gtttttctcg attggcggta ttcgtgccgg gcgcggttgc
     3001 gggagaccgc gttcttgcgc gtgtcactga agtccatacc cggtacgcgc gggcggacct
     3061 gatctccgtt gttacctcat ctccggaccg tgttccggcc gaatgcccac ttgcgggccg
     3121 ttgcggaggc tgccagtggc aaacgaccgc aatggcaagg cagcatgcgg cgaagcacgg
     3181 ggcggtcgtg gaaacgctcc gtcgagtcgg gaaatgggaa gagccgccgg ttcgtgacat
     3241 ccttccgagc ggcgagccct ggaggtatcg gaacaaggcc cggtacgctg tcgttacggg
     3301 agccgcaggg acaggaatcg ggtatttcgg cacgcgaacc cacgatcttg tcagtatttc
     3361 gcgttgcccc ctcaacatgg aacggcttga cgcactcctc gatgctgcgc acgaccttct
     3421 gatcaacgat ccccgatttg acgaccttcg cacgaccacg aactccctca ccgggagaac
     3481 gagcgcagcg acaggtgaga cagtgttgcg ggtggccgcc aaggcggcgt ccgactgggg
     3541 ggctttcgcc gaagctcttc ggcaacggat cccgttcgtg agcggcgtcg tcgttgcggg
     3601 tcatccgagg cgcggtgatc gtgtggtgtg gggttcgggc agcctgacgg aacgtgttcg
     3661 agggaacgag tatcgcgtgt cggccgcgtc atttttccag gtgaatccat tcgcggtcgg
     3721 caatctcgtg gacactgtcg cggacgctgc gggtctgcag ggaagcgaga accttgtgga
     3781 cgcgtatgga ggcgtgggcc ttttttcttt ggtaatt
//
LOCUS       DAOTNG010000300         3797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19265_length_3797_cov_4.805452, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000300 DAOTNG010000000
VERSION     DAOTNG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19265_length_3797_cov_4.805452"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5179_12165"
     CDS             <1..659
                     /locus_tag="P5179_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRS96016.1"
                     /translation="YTGSGGFLKKGTPDEVAIQTSINTRKGVERCIRYAFEYTRRRNR
                     RKTLTLCGKTNVLTFAFDLWERTFHEVGAEYPDIKREYAHVDATTMWMVKNPEWFDVI
                     VTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPIGGSAPKYTGQGVINPIAA
                     IGAAQMLLDTLGETKAAECLEKAIIEVVGTKMKSMSAGKMGYSTSQVGDLVVEALLKS
                     "
     gene            complement(741..1439)
                     /locus_tag="P5179_12170"
     CDS             complement(741..1439)
                     /locus_tag="P5179_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96017.1"
                     /translation="MRGRIAFVFAALLALCIPARSAVTVKVDMDSITVGRDSNIVKMI
                     KILRGDSAQVDVQNPLAAVPPEAWAKLTPEQTQDLIETSLALQRPSRHETIPSSDSRV
                     EIIVPVAFFAACVLALFFLMRWRIHQSKLVHEQRLAMIAQGIVPPDMATQPKPAAPRV
                     QKMIIWGWIMALGGVGLSIANVLEDGWSEIGWGLVIMLVGVGLLMAARYIGGQPVTGP
                     KEGDVPPSQDSGTT"
     gene            complement(1573..2151)
                     /locus_tag="P5179_12175"
     CDS             complement(1573..2151)
                     /locus_tag="P5179_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS96018.1"
                     /translation="MADQLKMRRRSNADVPEVALPSGYRLRHYVQGDEGVWADIINRA
                     GALGEYTEEKVVDTLTGQPRFHPEGLLFVTTDADEPVATACAWLGTHDDWRAGSVHMV
                     AVVPEHQGKRLSYWVCAAVLHVFRDWGVPDVYLTTDEFRTAAVKVYIQLDFEPVIRTP
                     EHYQRWCAVYRSYGMEERATALEKEAALGACW"
     gene            2212..>3797
                     /locus_tag="P5179_12180"
     CDS             2212..>3797
                     /locus_tag="P5179_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRS96019.1"
                     /translation="MKAFWRNVALFTLVFLVVFTSIIAITVHLLSRDLPDLAAVEQIR
                     PKLSTVITSADGQILKTFAIQKRTLVAFDDLPPALTDALLASEDREFWSHWGVNPISF
                     VRASLISLVTLSPPRATSTLTQQLARDLFLTKERSLIRKIKEAIVATRIERTYTKKEI
                     IQLYLNQVYFGAGAYGVQAAARTYFGCDARDLTTEQCATLVGMLPAPNYYNPIRNPER
                     ALARRNVVLGAMGATRKLPKSEVARLQALPLVTTPGLEELGIAPYFTEYIRIRLSEQL
                     QKSDSLRIRFAQVAGLPDTASADQLIYEGGFVIETTLDSRLQQIAERVLFARIDSMQA
                     YYDRAVRAQNNINSPWVDRERSLATGSIVAKRVQAALLAMDVRTGAILAMVGGRDFDA
                     TKFNRAVQALRQPGSSFKPFVYTAAIDNGFSPVTEIPNQPVTLSDPQRGEWRPENYDG
                     SIGGPMTLREALAKSVNLVAIRLLLQTTPRLVIQYAENMGIKSNLPPYPSLALGVGEV
                     RLVDLTAAYSVFPHQGIYVEP"
BASE COUNT          821 a         1122 c         1096 g          758 t
ORIGIN      
        1 tgtacacggg gtctggcggc ttcctgaaaa agggcacgcc ggacgaggtg gccattcaga
       61 catccatcaa cacgcgcaaa ggcgtcgaac gctgcatccg ctacgcgttt gaatacacac
      121 ggcggcgcaa cagacggaag acactcaccc tttgcgggaa gacgaacgtc cttacgttcg
      181 ccttcgacct ctgggagcgc acgtttcacg aagtgggtgc ggaatacccg gacatcaaac
      241 gcgaatacgc acacgtagac gccacgacga tgtggatggt gaagaacccc gagtggttcg
      301 atgtaattgt gaccgacaac atgttcgggg acattatcac tgacctcggc gcgatgattc
      361 agggcgggat gggcgtcgcc gcgggtggga acatcaaccc ggaaggcgtg agtatgttcg
      421 agccgatcgg tggctcggca ccgaaataca cgggtcaggg ggtaatcaat ccgatagccg
      481 ctatcggtgc cgctcagatg ctgctcgaca cactcggaga aacgaaggcc gcagagtgcc
      541 tggaaaaggc gatcatcgag gtagtcggaa cgaagatgaa gagcatgtcc gcgggtaaga
      601 tggggtactc gaccagccag gtcggggacc tcgttgtgga agcgttactg aaaagctaac
      661 gcgtaccgtg ctgtatgaaa caggaagagg agcgcagcta caagccgcgc tcctcttgtt
      721 tttggctcca tgattttacc ttacgtggta ccgctgtcct gcgatggcgg aacatcgccc
      781 tctttcgggc cggtcaccgg ttggccaccg atgtaccgcg ccgccatgag cagacctacg
      841 cccacaagca tgatcaccaa cccccaaccg atctcgctcc agccgtcctc aagcacgttg
      901 gcgatggaca gccccacgcc gccgagcgcc atgatccatc cccagatgat catcttctga
      961 acgcgaggcg cggcgggttt gggctgagtc gccatatcag gcggaacaat tccctgcgcg
     1021 atcatggcca gccgctgttc gtgcaccagt ttggactggt gtatccgcca ccgcatgaga
     1081 aagaacaggg caaggacgca cgcggcgaag aatgccacgg ggacgataat ctctacccgg
     1141 gaatcggaag aggggatcgt ttcatggcgg gaaggacgtt gaagagcgag agacgtctcg
     1201 atcagatcct gcgtctgctc aggcgtcagt ttggcccagg cttccggcgg caccgcggcc
     1261 agcgggttct gcacatcgac ctgagcgctg tctcccctca ggattttgat catcttcacg
     1321 atgttcgaat cacggcccac ggtgatagaa tccatgtcca ccttgaccgt gacggcggat
     1381 cgcgccggga tacagagcgc cagcagcgcg gcaaacacga atgcaattcg gcccctcata
     1441 ggttcgacct cccttggaaa tgcccacctg cgcacgttgt agtttcgcgc ttcagatggt
     1501 aagaccggat gtgcgcggga aatgttacat ccaccaatcg ttcctcgtcg ggaacctgac
     1561 ccgcggtgcc ggttaccagc atgctccgag cgcagcttcc ttttccagag ccgtcgcgcg
     1621 ctcttccatg ccgtacgagc gatagacggc gcaccaccgc tggtagtgct cgggggtacg
     1681 tatcaccggt tcgaaatcca gctggatgta caccttcacc gcagccgtac ggaattcgtc
     1741 ggtagtgaga tagacgtccg ggactcccca atcccggaac acatggagca cggcggcaca
     1801 cacccagtag gagagccgtt tcccctggtg ttccggaaca accgcgacca tgtggaccga
     1861 tccggcgcgc cagtcatcgt gcgttccgag ccacgcacag gccgtggcga ccggttcgtc
     1921 tgcatccgtt gtcacgaaaa gcaggccttc agggtgaaac ctcggttgtc ccgtcagagt
     1981 atctaccacc ttctcttccg tatattctcc taaggctcca gcacggttta tgatgtcggc
     2041 ccacacgcct tcgtcgccct gaacgtaatg cctgaggcgg taccccgaag gcaatgcgac
     2101 ctcgggcacg tccgcgttgg aacgacgacg cattttcaac tggtctgcca tttcacctcc
     2161 gtagagaact cataatcgtg tgctgaatgg ctattcgagg ttaagcaagg gatgaaagcg
     2221 ttctggcgca atgtggctct tttcacgctt gtgttcctcg tagttttcac gtcgattatc
     2281 gcgataacgg tgcaccttct aagccgtgat ctccctgacc tggcagcagt tgagcagata
     2341 cggccgaagc tttccaccgt aatcacatcc gccgatggcc agattctgaa gactttcgcc
     2401 atacagaagc gcacgctggt cgctttcgat gatcttccac ccgccctgac tgatgctctg
     2461 ctggcgagcg aggatcgcga attctggtcg cactggggag tgaatccgat cagcttcgtt
     2521 cgcgcttctc tcatcagcct ggtcacactg agcccgccga gagccacaag caccctcacg
     2581 caacagcttg cgcgcgatct tttcctgaca aaggagcgca gtctgatcag gaagataaaa
     2641 gaggcgattg tcgcgacccg aattgagcgt acgtatacaa agaaagagat cattcagctc
     2701 tacctcaacc aggtctattt cggtgcgggt gcgtacggtg ttcaggccgc agcgaggacc
     2761 tatttcggct gcgacgcgag agaccttacc accgagcagt gcgccacgct ggtgggaatg
     2821 ttgccggctc cgaattacta caaccctatc aggaacccgg aacgcgcact ggcgcggaga
     2881 aacgttgttc tgggagccat gggagcaacc aggaaactgc cgaaatccga agttgcccgc
     2941 ctgcaagcac ttccgctggt gactaccccg gggctcgaag aactgggcat cgctccgtat
     3001 ttcacggaat acattcgcat caggttgtca gaacaacttc agaagtctga ttcgctccgc
     3061 atccgttttg cgcaggttgc aggacttccc gacaccgcct cggcagacca gctcatctac
     3121 gaaggtggct tcgtgatcga aaccaccctg gacagccgat tgcagcaaat agcggagcgc
     3181 gttctgtttg cgcggatcga ctcgatgcag gcgtattacg atcgtgccgt gcgcgcccag
     3241 aacaacatca acagcccgtg ggtagaccgg gaacgaagcc ttgcgacggg ctccatcgta
     3301 gcaaagcgtg tgcaggccgc gcttctcgcg atggacgtgc gcacgggcgc aattcttgcc
     3361 atggtcggtg gccgtgattt tgacgcgacg aagttcaacc gggcggttca ggcattgcga
     3421 caaccgggaa gcagtttcaa accgtttgtc tacacggcgg caatcgacaa cggtttcagt
     3481 cccgtcacgg aaattcccaa ccaaccggtt actctgtcgg atcctcagcg gggcgaatgg
     3541 cgtcctgaga actacgatgg gagtatcggc ggcccgatga cgctcaggga agcgcttgcc
     3601 aagtcggtga acctggtcgc aattcgtctc cttctccaga caactccacg gctcgtgatt
     3661 cagtatgccg agaacatggg cataaaaagc aatcttccgc cgtacccatc gctggcgctg
     3721 ggcgtgggtg aagtgcggct ggttgacctc actgcggcgt attccgtgtt cccgcaccag
     3781 ggcatctatg tggagcc
//
LOCUS       DAOTNG010000301         3770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19405_length_3770_cov_4.801077, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000301 DAOTNG010000000
VERSION     DAOTNG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19405_length_3770_cov_4.801077"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1459
                     /locus_tag="P5179_12185"
     CDS             275..1459
                     /locus_tag="P5179_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS96020.1"
                     /translation="MARCLPAGGENLFQRIKRVRNEYEKRTGKEAINLSVGEPDGIPT
                     VATRAAAATACMSTDRRMHLYQDNGAPEGFARGLVEYHVGPEIAGRPELAFLPIPGIK
                     PMIGLLPLACGANAGKTVVVAGTTRPGYPVIRTWSGYLGCAYHDWPLYAANGFRPELN
                     DAPAGVTLAVFNYPNNPTGATMSVDKWRELCAWAETHGVRLANDAAYGGLVHGSHAHL
                     SQVATEFPKLEWIELFSASKSHNATGWRVGVAFGTADFVADLTTIKGNTDSGFVAPMA
                     TGVMRAIEVDRAGLDTIRTLYGKRITILVDLLSPHLELAAAPEAGFFTFWRIPREAFG
                     HPVRTADEFNALMIENCGIVGVPYTGSDGEYIRYAVCFPVEREDVQAAIRSALASAAL
                     KR"
     gene            1560..1636
                     /locus_tag="P5179_12190"
     tRNA            1560..1636
                     /locus_tag="P5179_12190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1594..1596,aa:Ala,seq:cgc)
     gene            1732..1831
                     /gene="ffs"
                     /locus_tag="P5179_12195"
     ncRNA           1732..1831
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5179_12195"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1870..3567
                     /gene="dnaX"
                     /locus_tag="P5179_12200"
     CDS             1870..3567
                     /gene="dnaX"
                     /locus_tag="P5179_12200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRS96021.1"
                     /translation="MADERFRVLARKWRPATFSEVVGQEHVCRSLTNAIRLGRIHHAF
                     LFCGPRGVGKTTVARILAKALNCVDGPTATPCLSCYSCKSIADGSSLDVMEIDGASNN
                     NVDQVRDLRETLGYATAQSRYRVIIIDEVHMLSKAAFNALLKSLEEPPPHVVFVFATT
                     EATKVIPTVQSRCQRYDFRRLSTRDIRDQLTMICREEKVDVEPAGIDLIAQYADGAMR
                     DGQSLLDQVIAATEGHVTASEVADLIGAVERTVFFDLVDAIARRDVSATISAVADTVF
                     RGRSLAQFLSGLMSHLRCLLACAANARNALSDFEPEELERLSAQARKFSDRDILRMLS
                     LAADAESLLEKSAVPQVRVELALLKMVYMEKSVELSDLVGRLDTLLTGPPAAPPHPVA
                     RGPAPPETAETRTATISTPSAVPAKRIAADLTSPDHPPPVRRESEEDQPGTPGLGLLT
                     ARWPDIVDSVKSASSAVPWFHQVRPTGIDGDTVILEVDGAFFAERVSRMDESLLGAIR
                     AHFPAVRRISVRAVDKSGQNSDVSETVRRAGSLIQGVVEEEPVIGKLIESLGLELIE"
BASE COUNT          771 a         1084 c         1145 g          770 t
ORIGIN      
        1 ggcacgaagc atcgagccac ttgaccgtcg tgatgatgga atttgtcata cctattacac
       61 tgatcacgct cgcgacgatt acggtaaggc acatgcgtga aaagaaccgt acggttcctc
      121 aatgaccggg cgcggggtgc cccggaacgg ggtgtcccga aactgggggc cccgaaacgg
      181 ggaagtggaa atctgcaaat gtaacgcctt tacggggtag taatttacaa ttggaactcg
      241 cccgtttctg attctcacaa cacgaggttt ctgaatggca agatgtctgc ccgcaggtgg
      301 tgaaaacttg tttcaacgca tcaagcgcgt gcggaatgag tacgaaaaaa gaactggaaa
      361 agaggccatc aatctcagcg ttggcgaacc ggacgggata ccgacggtgg caacgcgcgc
      421 cgctgcggct acggcgtgca tgtctacgga tcggcgcatg cacctctacc aggacaatgg
      481 cgcgccggag ggctttgccc gtggactcgt ggaataccac gtggggccgg agatcgcggg
      541 aaggccggaa ctggcattcc tacctattcc cggcatcaaa cccatgatcg gcctgttgcc
      601 tctcgcatgc ggcgccaatg caggcaaaac cgttgtggtg gccggaacga caaggccagg
      661 atatcctgtg attcgcacgt ggagcgggta tcttggctgt gcgtaccacg actggccact
      721 ctacgcggca aatggctttc gaccggaact caatgacgcg cctgcgggag tgacgcttgc
      781 agtgttcaat tacccgaaca acccgaccgg cgcgacgatg tcggttgaca aatggcgtga
      841 gttgtgcgcc tgggcggaga cacacggagt gcgccttgcc aacgatgcgg cctatggagg
      901 gcttgtccac ggcagccatg ctcatctgtc gcaggttgcc acagaattcc cgaaactgga
      961 gtggattgag ctgttcagtg cgtcgaaaag ccataacgcg accggatggc gcgtgggtgt
     1021 cgcgttcgga acggcggatt tcgttgccga ccttacgacg atcaagggca atacggacag
     1081 cggcttcgtc gcgccgatgg caaccggcgt tatgcgggcc attgaggtgg atcgggccgg
     1141 attggatacc atccgcacgt tgtacgggaa gcgcatcacc atccttgtgg acctcctttc
     1201 gccgcacctt gaactcgccg ccgcacctga ggccggtttt ttcacgttct ggcgcattcc
     1261 acgagaagcg ttcgggcatc ctgtccgcac cgcggacgaa ttcaacgcgt tgatgatcga
     1321 gaactgcggt atcgtgggtg tcccttacac cggttccgac ggagaataca ttcggtacgc
     1381 ggtgtgtttc cctgttgaac gagaggacgt tcaagcagcg attcggtcag ctctggcttc
     1441 cgcagcactg aagcgttgac ggacgtgtgc ggcggacgaa agtgccattt cttccgttgc
     1501 accgtgcgtg tggaaggctt atatttgcta tagcaatctt cgagaccttt gcgagtccag
     1561 gggcagtagc tcagtttggg agagcgcatc cctcgcacgg atgaggtcgt cggttcgagc
     1621 ccgatctgct ccaccaattc tttttctgca ggactgatgt cagaacgaac ggctcagtcc
     1681 tgcttacatt atcgagtccc cgagtatcgg ctccatgtcg gcggcgggga tggccaggcc
     1741 ctgcgcggcg gacatccgct gaaccccgcc aggaccggaa ggtagcaacg gtaggcgatc
     1801 ctgttcgagc gccgcaggta gtctggcctt ctccgccgcc cgcattcacc cgacacaccg
     1861 agaccggcga tggcagatga gcgctttcgc gttctggcgc gcaaatggcg tccggctacg
     1921 ttcagcgagg ttgtcggtca agagcacgtg tgccgcagcc tgacgaacgc gattcgcctc
     1981 ggccgcatcc accatgcgtt cctcttctgc ggtccgcggg gagtcggaaa aaccaccgtt
     2041 gcgcgcatcc tggccaaagc gctgaactgc gtcgatggtc ccactgcgac gccctgcctt
     2101 tcctgctatt cgtgcaaatc catcgcagac ggaagcagcc tcgacgtgat ggaaatcgac
     2161 ggcgcttcaa acaacaacgt cgatcaggtt cgggatctgc gcgagacgct cggatacgca
     2221 actgcccaga gccgataccg cgtcatcata atcgacgaag tgcatatgct ctcgaaggct
     2281 gcgttcaacg ccctgttgaa atcgctcgaa gaaccacccc cgcacgtggt ctttgttttt
     2341 gccaccacgg aagcaacgaa agtcatcccg actgtccaat ccaggtgcca acgttacgac
     2401 tttcggcgcc ttagcactcg agatattcgc gaccaactga cgatgatctg ccgcgaagaa
     2461 aaggtagacg tcgaacccgc agggatcgac ctgatcgcgc agtacgctga tggcgccatg
     2521 cgagacgggc agagtctgct ggaccaggtg attgccgcga ccgaaggtca cgttaccgcc
     2581 tcggaggttg ccgacctcat cggtgcggtc gaacgcaccg tgttttttga cctcgtggac
     2641 gcgatcgcca gaagagacgt ttcggccacc atttctgcag ttgcagacac ggtgttccgc
     2701 ggaagaagcc tggcgcagtt tctttcgggg ttgatgtccc atttgaggtg tctgctggcc
     2761 tgtgcggcca atgcccggaa tgccttgagc gatttcgagc cggaagagct tgaacggctt
     2821 tccgcccagg cgcgcaaatt ctctgaccgg gacattttga ggatgctgtc tcttgcggcg
     2881 gatgcggaat cgcttctgga aaagtccgcc gtgccgcagg ttcgtgtaga acttgcgttg
     2941 ctcaaaatgg tttacatgga gaaatccgtt gagctctcgg accttgtagg gcgattggac
     3001 acgcttctga cgggtccgcc tgcggcgccg cctcatcctg tcgcacgggg acctgctccg
     3061 cctgagacgg cagaaacgcg aacagcgacc atctccacgc cttcggcggt tcccgcaaaa
     3121 agaatagccg ccgacctcac atctcctgac cacccaccgc cggttcgccg ggaatccgag
     3181 gaagaccagc ctggaacgcc gggtcttggt ctgttgactg cccgctggcc ggacatcgtg
     3241 gattccgtga aatcggcctc ctccgcagta ccttggtttc atcaggtgcg tccgaccgga
     3301 atcgatggcg acacggtgat tctggaagtt gacggggcat ttttcgccga acgcgtttcg
     3361 cgcatggacg agagcctgct cggcgcgatt cgtgctcatt ttccggccgt acgccgcata
     3421 tcggtccgag ctgttgacaa gagcggccag aacagcgacg tttctgaaac cgtacgccgg
     3481 gccggttccc tgattcaggg cgttgtcgaa gaggaaccgg tcatcggcaa gttgatcgaa
     3541 tccctgggtc ttgaactgat cgaatgagag cctgatgctc gattcctgcg aatcgacaga
     3601 cacccaaacc ggagcataag gcggaatgtt cggaaacctt ggcaactttg ccgcgttcgt
     3661 caaaagcgct caggaaatgc agaagcgcgt ggcggaggtt caggaggtct tgaaggctgc
     3721 gaaagtcgag ggctccgccg gcggcgatct ggtcagggtc accgcaaacg
//
LOCUS       DAOTNG010000302         3724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19692_length_3724_cov_4.213410, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000302 DAOTNG010000000
VERSION     DAOTNG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19692_length_3724_cov_4.213410"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..720)
                     /locus_tag="P5179_12205"
     CDS             complement(118..720)
                     /locus_tag="P5179_12205"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRS96022.1"
                     /translation="MAFELPSLPYPDTALEPNYSAKTFSFHHGKHHKAYVDNLNKLIA
                     GSELEGKTLEQIILATANDPAKTGIFNNAAQVWNHTFFWNCMKPAGGGKPSGALASAI
                     DAAFGGYDKFVEQFKAAAVGRFGSGWAWLVLDNGALKIVSTPNGETPMTKGQKALLTV
                     DVWEHAYYLDYQNRRPDFVQTFLDHLVNWDFVAQNFEKAK"
     gene            complement(888..2693)
                     /locus_tag="P5179_12210"
     CDS             complement(888..2693)
                     /locus_tag="P5179_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRS96023.1"
                     /translation="MPQSKQFAPRDVVLEPGLFRDRYELNRRYVMSLKNENLLQNHYL
                     EARIWQTSFRNTHNGETSWGEDRHWGWESPTCQVRGQFLGHWLSAAARIAETGDDSEA
                     RAKLNKVVAELARCQQANGGEWVASIPPSYLEWTAKGQPTWAPHYVVHKTLMGLFDAY
                     AISGNEQALEILSKAAEWFVRWSSKFDPDAMNNILDTETGGMLEVWANLYGVTGKQEH
                     LDLVYRYDRPRLFDRLLNGEDALTNQHANTTIPEAHGAARAWEVTGDERWRRVVEAYW
                     ECAVTNRGYFCTGGQTCGEVWTPPYAFFARLGNKNQEHCTVYNMMRLADYLFRWTGHA
                     QYADYIERNLYNGILAQQNPSTGMVSYFLPLCAGGKKRWGSPTNDFWCCHGTLVQAHT
                     LHNAYGFYKSDDGLILANYIPSSVTWRREGGVVVRARQTVDPEVSAQSLPKRLVVNLV
                     VSCSTQTEFALQVRVPWWVSGEMEIWVNGVRLDTNAVAPSSFVTIRRTWQQETIRIVI
                     PRSLHSSPIPDMPDMVAFMDGPEVLVGLVHEETRLAGNRDEPGSFLTPDNEREWTTWR
                     RTYRTTRQSRGVRFVPLHAIQDETYTVYFPVEEPR"
     gene            2859..>3724
                     /locus_tag="P5179_12215"
     CDS             2859..>3724
                     /locus_tag="P5179_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96024.1"
                     /translation="MGDRVFEKLADPSLLARSPIFIFAADALTAYGLTGIGIEKTPSP
                     HYRTRPEYLDLQRAILGDGYIDGLLMSPADAENLAFEEKLFDSSPVTPIVRMNAETRI
                     WSPRHGKYGAQPSRPFTTIRTPEAGRYCDEAMACAKDCKIKLGLYSITLNNDTDADER
                     TLSAYLDFAWDVAQTPDFHHFLEVFLPNMSQPGMDLQAMGEYVADSIVRTMAHLRKRQ
                     RPLFIKTVYTTPAVWKALVEFDPTLVIGALGGQRINAKRTLELAHTTTEYGGKVILFG
                     RAIFQEENPRAM"
BASE COUNT          784 a         1097 c          989 g          854 t
ORIGIN      
        1 cataagaaca gtttttgggg gcacgctcgc ctcctctcgg ttatcttggg gcttgcggaa
       61 aacgaaagcg ccgtgcagaa ggttctcgca cggcgctcat ttccgaccac agtggtttca
      121 tttcgccttt tcgaagttct gagcgacgaa atcccagttt acgaggtggt caaggaaggt
      181 ctgcacaaaa tcgggccggc ggttctggta atccaggtaa tacgcgtgct cccagacatc
      241 caccgtaagc aaggccttct gtccctttgt catgggtgtc tcaccgttag gcgtacttac
      301 gattttcagc gcgccattat cgagaaccag ccacgcccag ccgcttccaa atctccccac
      361 tgcggctgct ttgaactgct ccacaaattt gtcgtacccg ccgaacgccg cgtcaatggc
      421 cgaagcaagg gctcccgaag gctttcctcc gcccgccggt ttcatgcaat tccagaaaaa
      481 cgtgtggttc cagacctggg cggcgttgtt gaaaattccg gttttcgcag ggtcattcgc
      541 ggtggccagt atgatctgtt ccagcgtttt tccttccagt tccgacccgg ctatcagctt
      601 gttcagattg tcaacatacg ctttgtgatg tttgccgtgg tggaaactga acgtttttgc
      661 cgaataattg ggttccagtg cggtgtctgg gtacggaagc gaaggaagtt cgaatgccat
      721 ggcgccctcc tttgtggtga aaacatggac ggacttgaga atgaaactgt taatttaacg
      781 gcaaaaccat gcaaatgagt tccatctctt tccgccccga atgtgatgcg tcgccacatg
      841 gtattcgccg cggctgtcgt cacggatgtc atccgaacat aaccacatca ccggggttct
      901 tccactggga aatacacggt atacgtttca tcctgtatcg catggagcgg cacaaatcgc
      961 acgccgcgtg attgccgtgt cgttcggtag gttcgtcgcc atgtcgtcca ttcgcgctcg
     1021 ttatccggtg tcagaaagct gccgggttca tctctgtttc cggcgaggcg cgtttcttcg
     1081 tgaacaagcc ccacaaggac ttcgggacca tccatgaatg ccaccatatc cggcatgtcc
     1141 ggaatcgggg aagagtggag gctcctggga ataacaattc ggatcgtctc ctgctgccac
     1201 gttctgcgga ttgtcacgaa cgaagaaggc gcaacagcgt ttgtgtccag acgcacaccg
     1261 ttcacccaga tctccatttc tcccgacacc caccagggca cgcgcacctg caaagcaaac
     1321 tcggtctgtg tggaacagga caccacaagg ttcaccacca ggcgctttgg caacgactgg
     1381 gcagacacct ctggatcaac agtctgtcgg gcccgtacga caacaccgcc ctccctccgc
     1441 caggtaacac tggacgggat gtagttcgcg agaatcagcc cgtcgtcaga tttgtaaaag
     1501 ccatacgcgt tgtgcaacgt atgagcctga accagtgtgc cgtggcaaca ccagaagtca
     1561 tttgtgggtg atccccagcg ctttttccca cccgcacaca gcgggaggaa gtacgacacc
     1621 atccccgtcg acgggttctg ctgcgcgaga ataccgttgt acagattgcg ctcgatgtag
     1681 tccgcgtact gcgcatgtcc agtccagcga aacagataat ccgcaagccg catcatgttg
     1741 tagacggtgc aatgctcctg gtttttgttc cccaaacgcg cgaaaaacgc ataaggcggc
     1801 gtccacacct caccgcacgt ctgtcccccc gtgcaaaagt atcctctgtt cgtcactgcg
     1861 cattcccagt acgcctctac aacccgtctc caccgctcat cgccggtgac ttcccacgcg
     1921 cgtgcagcgc cgtgagcttc ggggatggtc gtgttggcat gctgatttgt gagagcgtct
     1981 tccccgttca gaagccggtc aaaaagccgt ggacgatcgt atctgtacac cagatcaaga
     2041 tgctcctgtt tgcctgtgac accgtacaga ttcgcccaga cttcgagcat tccgcctgtt
     2101 tctgtgtcaa gaatgttgtt catcgcgtca ggatcgaatt tgctcgacca gcgaacgaac
     2161 cattccgcgg ctttcgaaag gatctccaga gcctgctcgt tccccgaaat ggcatacgcg
     2221 tcaaaaagac ccatcaaggt cttatgaacc acgtagtgcg gtgcccaggt gggctgacct
     2281 tttgcggtcc attcgagata tgacgggggt atggaggcca cccactctcc accatttgcc
     2341 tgttggcatc ttgcgagttc cgccaccacc ttgttcagct ttgcgcgtgc ttcagagtcg
     2401 tcgccagttt cggcgattct ggcggccgcg gatagccagt gcccgaggaa ctgcccgcga
     2461 acctgacacg tcggggattc ccatccccag tgccggtctt ctccccagct tgtctctccg
     2521 ttatgcgtgt tgcggaagct ggtctgccag atccgcgctt ccagatagtg gttctgcaaa
     2581 aggttctcgt tcttcaaact catcacgtac cgccgattca gctcgtagcg gtcgcgaaaa
     2641 agccctggtt ccagtaccac atcacgcggt gcaaactgct tcgactgcgg catcatccgt
     2701 cctccaaacg tggctctctc gtgtctgcca aaatgaggtt aaggacggcc atgccgtccg
     2761 cacaacgggt ccgcggcggg ttgacttcgc atgccaggtc tcgtatttct gaagcgtgca
     2821 cgcagagtcg gtcgaacctt tgatcaggaa ggcacgctat gggcgacagg gtttttgaga
     2881 aactggcaga tccttctctt ctcgcacggt ctccgatatt tattttcgcc gccgacgcac
     2941 tgaccgcata cggtcttacc ggaatcggga tagagaaaac gccgtcacct cattatcgca
     3001 cgcgtccgga gtatctcgat cttcagcgag ctattctcgg ggacggatat atcgacggtt
     3061 tgttgatgtc acctgctgac gcggagaacc tggcgtttga agaaaagttg tttgattcgt
     3121 cgcccgtaac accaatcgta cgcatgaacg cagaaacgcg tatctggagt cctcgccatg
     3181 gtaagtacgg tgcgcagccc tcacgtcctt ttaccacgat tcgtacgccg gaagcgggca
     3241 gatactgtga tgaggcgatg gcctgcgcaa aggattgcaa gatcaaactc gggctctact
     3301 caatcaccct caacaacgat accgatgccg acgaacgaac cctgagcgcc tacctcgatt
     3361 tcgcgtggga cgttgcgcag actccggatt tccatcattt cctcgaagtg ttcttgccga
     3421 acatgagcca acccggtatg gatctgcagg ccatgggcga atacgtcgca gactccatcg
     3481 tgaggacgat ggcgcatctg cggaaacgcc aacggccgct gttcataaaa actgtgtata
     3541 ccacaccggc ggtctggaag gcgctggtgg agttcgaccc caccctggtc atcggcgcac
     3601 tcggcggcca gcgcatcaac gcgaagcgga cgcttgagct cgcgcacacc accactgaat
     3661 acggtggcaa ggtcattctg ttcgggcggg cgatttttca ggaggaaaac ccgcgcgcga
     3721 tgtg
//
LOCUS       DAOTNG010000303         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19799_length_3705_cov_4.817534, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000303 DAOTNG010000000
VERSION     DAOTNG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19799_length_3705_cov_4.817534"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..798)
                     /gene="dtd"
                     /locus_tag="P5179_12220"
     CDS             complement(349..798)
                     /gene="dtd"
                     /locus_tag="P5179_12220"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRS96025.1"
                     /translation="MRAVVQRVSSAHVAVDGQTIGAIGPGLVVLLGVAEGDSDADVAY
                     LAEKIANLRIFEDAAGKMNRSLLETGGALLAVSQFTLLGDCRKGRRPSFIEAARPEEG
                     KRLYDAFCDAVRAFGVRVETGRFQAMMQVALVNDGPVTILVDSRKVF"
     gene            complement(804..1841)
                     /locus_tag="P5179_12225"
     CDS             complement(804..1841)
                     /locus_tag="P5179_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96026.1"
                     /translation="MTPREIVQMSLAFAYPPRIPFGMGSAGFPSDFRGVHHDPAPNRV
                     QQPWTQRDGYWDMIDMWGNTWRRLESITKGEVYKGVIQDSWDLLDTYEFPILDRAELF
                     EKAAAEVKQYHAEGYYVLGGMSWTFDTARYMRRMEVFLRDCAEEPDRVSYLLNRIADV
                     YERVIERYADIGVDGISTGEDWGTQDRLLVSPPMFRRLFKPVFARLCGVAKSRGLSIH
                     FHSCGFVRDVIGDWIEVGIDSFKFDQPELHGIDWLAKHYGGKMHIWSPVDIQTTLQTR
                     DHARIEAAAREYIEKLALPFGGGVIAGYYGSNEAIGLTPEYQETASRAFMKYGDPALW
                     TGRPASRTEKA"
     gene            2037..3479
                     /locus_tag="P5179_12230"
     CDS             2037..3479
                     /locus_tag="P5179_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96027.1"
                     /translation="MPCGLIRVFALLCMLPAWSAAQVYSFEEDAPPDSSVAPLDSAAV
                     ERTWNAVLSGARQHSPGLVRPYVAERYRNTRLVTSLVALGSVQRFRVLRMDVYPAFAR
                     LNLEVTENFRRSYHSFYFVRESGKVVLTYRWHAYSQHWVERRSPHFTFVYNPYKDSIG
                     RGVAVPTNMAATLLERHRAAMANLLNPASAERIYIYLVNTKAEMMSLFGSTADEAFIE
                     PETGAIVSDFPHGVFQLVTTRLLFQVSGDSLGKLNSTGNDRLCRLFINGVSTFGDGNG
                     GYVRGKSAVFMVRTLISQGRYIPLRDALSSPDNPLVVAEAGAFVRFLVSEYGASKFRS
                     AARSIHRDTDFIPVLERVYGVRFPALERDFRVWVVRQNEEVLEPSELVEFRILTHLWD
                     VQRVGGTRVYVDRGMSFPPISAIGRVMTLFQNRVATQPATIPYIYLAASENQLAEFYI
                     QGSAYVSEHVMIAASTEALERALRSRRERR"
BASE COUNT          682 a         1129 c         1135 g          759 t
ORIGIN      
        1 gccgcctcgt gcggacgttg gtgggccagg cattcctgcc tggccaccac gaggttgtat
       61 gggacggata tgacgctatg ggtcgcgcgg ttgccagcgg ggtgtatgta tacagcctca
      121 cggcggcgca gggagtggtg acgaggagga tggtgctggt gaggtaagtg agggtacggg
      181 cgccaagatg caggggccga cccccgtgtc ggcccgtgtg tccacccgtg atcataacgt
      241 gtgtgccggc gggggcggac acagagatcc gcccctacgt ttcaaggggg cgctctactt
      301 tgttcccccg cggtccggtg catattcaga caggggcgcg ttgccgcgtc aaaaaacctt
      361 cctgctgtcc acgagaatcg tcacggggcc atcgttcaca agagccacct gcatcatggc
      421 ctgaaagcgg ccggtttcca cgcgaacccc gaacgcccgc acggcgtcgc aaaatgcgtc
      481 gtacagtcgc ttaccctcct cgggccgcgc ggcttcgatg aaggacgggc gccgaccttt
      541 gcggcaatcc cccaggagcg tgaactgcga caccgccagc agcgctcctc cggtttcgag
      601 cagcgaccgg ttcattttgc cggcggcgtc ctcgaaaatc cgcaggtttg cgattttctc
      661 ggccaggtag gccacatccg cgtcggagtc gccctcggcc acgcccaaaa gcacgacaag
      721 tccaggcccg atggcgccga tggtctgacc atccacggca acgtgtgccg aactgacccg
      781 ctgaacgacc gcgcgcatgg ggactacgcc ttttccgttc tgctggccgg gcgccccgtc
      841 cacaacgcgg gatcgccgta tttcatgaac gccctggaag ccgtctcctg atactccggc
      901 gtgagcccga ttgcttcgtt ggagccgtag tacccggcga tgacgcctcc cccgaagggt
      961 agcgccagtt tctcgatgta ttcccgcgcc gccgcttcaa tgcgggcgtg gtcgcgggtc
     1021 tggagggtcg tctgaatgtc cacgggcgac cagatgtgca ttttcccgcc gtagtgcttg
     1081 gccagccagt caataccgtg aagctccggc tgatcgaact tgaaggaatc aatcccgacc
     1141 tcgatccagt cgcctattac gtcgcgcacg aaaccgcatg agtggaaatg gatgctcaga
     1201 ccgcggcttt tcgccactcc gcacaggcgg gcgaatacgg gcttgaacag ccggcggaac
     1261 atgggcgggc tgacaagaag gcgatcctgc gtgccccagt cttcgccggt agagatgcca
     1321 tccacgccaa tgtcggcgta ccgctcgatc acccgttcgt acacgtcggc gatgcggttc
     1381 agaaggtagg atacacggtc aggctcctcc gcgcagtcgc gaaggaacac ctccatacgc
     1441 cgcatgtatc gcgcggtgtc gaaggtccag ctcataccac cgagaacgta atacccttcc
     1501 gcatggtatt gcttgacttc ggcggcggcc ttttcgaaaa gttcggccct gtcgagaatc
     1561 gggaactcgt acgtgtcaag aaggtcccag ctatcctgaa tgacgccctt gtacacctcg
     1621 cctttggtga tactttcgag acggcgccac gtgtttcccc acatgtcgat catgtcccag
     1681 tacccgtcgc gctgggtcca cggctgctga acgcgattgg gtgccggatc gtggtgcacg
     1741 cctctgaagt cactcggaaa accggcgctg cccattccaa acgggatacg cgggggatat
     1801 gcgaacgcga ggctcatctg aacgatttca cgcggtgtca tgccggggct ccttgcgaag
     1861 gcaacggtgc gagagagtat gcgtggtctg aatgtaccgt tcgtcggaaa aaatcggaac
     1921 cggacggcat ggtgccacct cgcggtggaa cgggggggtg tgtatatttt tgagtacgcg
     1981 caaccgggcg ttgcgaactc caatcgtacc gtacttcgtg cgagcagata gatccgatgc
     2041 cgtgtggcct gatccgggta tttgcccttc tgtgtatgct tccggcctgg agcgcggcgc
     2101 aggtatactc gttcgaggaa gacgctcccc ccgattcctc cgttgcgcct cttgattccg
     2161 ccgccgtgga acgcacctgg aacgcggtgt tgtccggtgc ccggcaacac agcccggggc
     2221 ttgtccgccc gtatgttgcc gagcgctaca gaaacacgcg gcttgtcacc tcactggttg
     2281 cgcttggctc cgtgcagcgg ttccgcgtac tgcggatgga cgtgtatccc gcattcgcgc
     2341 ggttgaacct tgaagtcacg gagaatttcc ggcgttcgta tcactcgttc tacttcgtca
     2401 gagagagtgg taaggtagtt cttacctacc gctggcacgc gtactcccag cactgggtgg
     2461 aacggcggtc tcctcatttc acgtttgtct acaacccgta caaggattcc atcggaagag
     2521 gggtagccgt tccgaccaac atggcggcaa ccctgcttga acggcaccgg gccgcgatgg
     2581 cgaacctcct gaacccggcc tccgcggagc ggatatacat ctacctggtg aacaccaagg
     2641 cggagatgat gagccttttc ggctctacgg ccgacgaagc gtttatcgag ccggaaaccg
     2701 gcgccatcgt gtcggatttc ccccacgggg tttttcagct cgtaacaacg cggctcctgt
     2761 ttcaggtgtc aggggattcg ctcggtaagc tgaatagtac gggaaacgat cggttatgcc
     2821 gcctttttat caacggtgtt tcgacgttcg gagacgggaa cggcggatat gtaaggggga
     2881 aatcggccgt tttcatggtt cgcacattga tttcgcaggg gcgctacatt ccccttcgcg
     2941 acgcgctctc cagccctgat aatccccttg tggtggcaga ggcgggcgca ttcgtccggt
     3001 ttcttgtcag cgagtatggc gcttcgaagt tccggtctgc cgcgcgttcc attcaccgcg
     3061 acacggattt catacccgtg ctggaacggg tatatggcgt gagatttccc gctctggaac
     3121 gtgatttcag ggtgtgggtc gtccgccaga acgaggaggt gctggagccg tccgaactgg
     3181 tggagttccg cattctcacc cacctgtggg atgtccagcg tgtcggcggg acgcgtgtgt
     3241 atgtggatcg cgggatgtcg ttcccgccga tatccgcaat cgggcgggtg atgacgttgt
     3301 tccagaatcg cgtggctacc cagcctgcca cgattcccta catctatctt gccgcgtccg
     3361 aaaaccagct tgcggagttt tatattcagg gatcggcgta cgtgtcggaa cacgtaatga
     3421 ttgcggcgtc gacggaagca ctggaacgcg ccctgcgctc caggagagaa cggcgctgaa
     3481 cgagctctcc ccgcactccg gtccgcgcgg ctgactcgaa actgcgccct caacccgcgg
     3541 cgaagttctc ttccaggcgc cgctggaact cagtgatgac cctgcgtgcg tcgttccgtc
     3601 cgtcctgaac cagccacagc cacacgaggg gcgagaggac agcaaccacg tactcccacc
     3661 acgcaaacga caggtgccac ccgaacacgg aggttccggc aacct
//
LOCUS       DAOTNG010000304         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19800_length_3705_cov_4.231781, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000304 DAOTNG010000000
VERSION     DAOTNG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19800_length_3705_cov_4.231781"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3705)
                     /locus_tag="P5179_12235"
     CDS             complement(<1..>3705)
                     /locus_tag="P5179_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS96028.1"
                     /translation="KPGFRGTELGLADPEPLFPLRSRKPSASARPEQVGAWFRYRISN
                     ELSPQQVADSLNRIPGVVAQPNYLYRMLGTAPNDPGWQNQWGAQRIRLDEVWQQVQAD
                     AAKFPIIGIIDSGVDYRHPDLEANIWHNAIEQAGLPGVDDDGNGYVDDFVGWDFTDSP
                     GLSGLGDYLDRDNDPFDEMGHGTQVAGVAAAVVNNGVGIAGTAPNARIMCLRAGAQLI
                     NGSGYLEEDDIAAAVVYAVENGADIINMSFGDVVSTPLMADIVAYATAARVVLVAAAG
                     NENTPSLLFPAAYDETIAVGATGENDARATFSSYGENLDLCAPGVSIYTTNTYGAYRR
                     NGGTSFSAPFVSGAAAILLSRAPHLSPTEVRSILCRGVVDIGPPGWDEQTGHGRLDLP
                     TLLSVAQDPVAQILTPGRTKEVRNAVRWAVHVRGTPPVAWVLSYGVGAVPRVWTTCAS
                     GVLETAGDTLRGTYSTIDHPDTLFCLRLTVTDLLGRTGEDRTLYSLDRSPPVFRSQPV
                     VLPRWDGDTRRIFLQWETDDLTTGTVRIWRGAVGGSSPLEYSVSATDRAHFEEIPWQD
                     VDVGPFAVQVVARNASGMETESAFSTGTASAYTIPRSGWDEEDVRAGGILMPAGTGMP
                     PLPDIDGNGIPEAVIKLAHRAPYDTVSFFELPPGSPPVLRTESPVKMRPTAVVDIDGN
                     AKLDLIGFDYFDSAFRVRVVEIGQSSPWWERTSLVGPSVADVDGDGRAEIIAVVDSNR
                     ALLHVYEAAGPGRIELKAILQSPVNGYEGQFGIWRAAGDFDGTGRNSIVAGTVGGAVV
                     LFPSVGDDAFGVPRVIEGQGDATRVCAIGDANNDGRDDFAIVRHLENTKFTTDVAAFR
                     LEVHTGSTTPYFVREFHDPRTEGNGFSSGYLGGRRVVVLATPPHLYVLDPTGSGVDGF
                     LYYGETDSPSQPLVADIDGQGGAELLFPRGGALAVLRALASPRAPAAPRNVSVRVRDS
                     ISVVVSWNAEPGLHYRIWTGVGRDAPLSLRTEFADPVPPVVLTFTPPVQSEIRVAVQA
                     VNPAIADSIGGVSDAIVVRPHTGPQALCAARVSPSGVDVRFDLPLSALETTERDFLLR
                     AGAQEHRPSSLVWDRSGFRVLLLFRSDELPSQDVTLDYDVRDTSGAVGNGTIPVVSAT
                     REERPRVDFAMAIGSRTIRIAFDREVVRASLVADSITILPRVAITGVEAVDQFGRFFD
                     IHFGSALSPTQVYVLRFAGVHGVAGEVFSTG"
BASE COUNT          769 a         1202 c         1049 g          685 t
ORIGIN      
        1 acctgtggag aaaacctctc cggcaactcc gtgaaccccc gcgaaacgaa gcacgtagac
       61 ctgtgtcggc gaaagtgccg aaccgaaatg gatgtcgaag aagcgtccga actggtcaac
      121 agcttcgact ccggttatcg ccacacgagg caggattgtg atggagtcgg cgacgagcga
      181 tgcgcgcacc acctcgcgat cgaacgcgat cctgatggtg cgggaaccga ttgccatcgc
      241 aaagtcaact cggggccttt cctctcgagt cgcagacaca accggaatcg taccgttccc
      301 cacagcccct gaggtatctc tcacgtcata gtccagcgtg acgtcctgtg aaggcaactc
      361 atccgaacgg aacaggagca gcacccggaa cccggaccga tcccacacga gcgaactcgg
      421 cctgtgctcc tgtgcaccgg cgcgaagcag gaaatctcgt tccgttgtct ccaaggcgct
      481 cagcggaaga tcaaaccgca catctacccc actgggagaa accctcgccg cgcaaagtgc
      541 ttgcggccct gtgtgtggtc gaaccacaat cgcatcgctc acgccaccaa tgctgtcagc
      601 gatggcggga ttcaccgctt gcacggcgac acggatttcg gattgcaccg gcggggtgaa
      661 cgtcagcacc accggaggga cagggtctgc gaactcagtc cgaagtgaaa ggggcgcatc
      721 tcggccaaca cctgtccata tccggtagtg caagcccggt tctgcgttcc acgagacgac
      781 taccgagatc gaatcccgga cccggactga cacgttgcgc ggtgccgccg gcgcacgggg
      841 cgaggcgaga gcgcgaagaa ccgccaacgc gcccccccgc gggaacagga gctcagcgcc
      901 tccctgcccg tcaatgtcgg cgacgagagg ctggctcggg ctgtccgttt ctccgtaata
      961 caggaacccg tcaactcctg aacccgtggg atcgagaacg tacaggtggg gcggcgttgc
     1021 cagaacaacg acgcgcctgc cacccagata cccggaggaa aacccgtttc cctcggtgcg
     1081 cggatcgtga aattcgcgaa cgaaatatgg agtcgtgctg cccgtatgca cttccagtcg
     1141 aaaggctgcc acatcagtgg tgaacttggt gttttccagg tggcgcacga tcgcgaagtc
     1201 gtctcgcccg tcgttgttcg cgtcaccgat tgcacacacg cgcgtcgcgt cgccttgccc
     1261 ctcaatcact cgcggaaccc caaaggcatc gtctccgacg ctcgggaaca gcaccaccgc
     1321 accaccgaca gttcccgcaa cgattgagtt cctgcctgtg ccgtcgaaat cgcctgccgc
     1381 cctccagata ccgaactgcc cctcgtaacc gttcaccggg ctctgaagga tcgctttgag
     1441 ttcgattcta cccggcccgg cagcttcgta gacgtgcagc agcgcgcgat tcgaatctac
     1501 cactgcgata atttccgcgc gcccgtcgcc atctacatct gcaacacttg gtcccaccag
     1561 cgacgtccgc tcccaccagg gtgacgactg accgatctcc accacccgca cccggaacgc
     1621 agaatcgaag tagtcgaatc ctatcaggtc caacttcgcg ttcccatcga tatcaactac
     1681 tgctgtcggg cgcatcttga cgggactctc cgtccgaagg acgggaggag atcctggagg
     1741 aagctcgaag aacgacaccg tgtcatacgg cgctcggtgc gcaagtttga tgacagcttc
     1801 aggaatcccg ttcccatcaa tgtcgggcaa ggggggcatt ccggtgccgg caggcatcag
     1861 gattccaccg gcacgcacat cctcctcgtc ccagcccgac cggggtattg tgtacgcgct
     1921 tgccgtgccc gtcgagaatg cgctctccgt ttccatcccg gaagcatttc tcgcgaccac
     1981 ttgaaccgca aaaggcccca catcgacatc ttgccagggg atttcttcga aatgggcccg
     2041 gtcggtcgca gatacggaat actccagggg cgaagatccg ccaaccgctc cgcgccagat
     2101 gcgcaccgtg ccggttgtca gatcgtcagt ctcccattga agaaagattc tccgggtatc
     2161 accatcccat cgcgggagaa cgaccggctg tgaccggaac acgggaggcg aacgatccaa
     2221 tgagtaaagc gtgcgatctt cgcctgttcg ccccagcagg tcagtcaccg tcaggcgcag
     2281 gcagaaaagt gtatcaggat gatcgatcgt ggagtacgta ccgcggagag tatctcctgc
     2341 cgtttccaga acgccggacg cacacgttgt ccacacccgt ggaaccgcgc cgacaccata
     2401 gcttagaacc cacgcgaccg gcggagttcc ccggacatgt accgcccagc ggacggcatt
     2461 tcgaacctct ttcgttcgtc cgggggtcag tatctgcgcc accgggtcct gtgccaccga
     2521 caaaagcgtt ggcaggtcca gccttccatg gccagtttgc tcatcccaac ccggcggccc
     2581 gatgtcaacc acgccccggc aaagaatgga acgcacttcc gtgggtgaaa ggtgtggcgc
     2641 gcggctgagg aggatcgccg ccgcgcccga gacgaatggc gcggaaaagg acgtgccacc
     2701 gtttcggcgg tacgctccgt aggtgttcgt ggtgtagatg ctgacgcccg gcgcgcagag
     2761 atcgagattc tcgccataac tggaaaacgt ggcccgcgca tcattttcgc ccgtggcgcc
     2821 taccgcgatg gtttcgtcgt atgcagcggg aaagaggagc gaaggtgtgt tctcatttcc
     2881 tgcggccgca accaagacca cccgcgcagc cgttgcgtac gccacgatgt ccgccatgag
     2941 aggcgtagac acaacgtcgc caaaactcat gttgatgata tctgcgccgt tttccaccgc
     3001 gtagacgacc gcggccgcga tgtcgtcttc ctcaagatat ccgctcccgt tgatgagttg
     3061 cgctcccgct ctgaggcaca tgattctggc gttgggggca gttcctgcaa tacccacccc
     3121 gttgttgacg acagccgccg caactcccgc aacctgcgtt ccgtgtccca tttcatcgaa
     3181 cgggtcgtta tcacggtcca gataatctcc aaggccggac aatccgggag agtccgtgaa
     3241 atcccatcct acgaaatcgt cgacgtaccc gtttccgtcg tcatccaccc ccggaagccc
     3301 cgcctgttca attgcgttgt gccagatgtt ggcttccaga tcaggatgcc ggtagtccac
     3361 cccgctgtcg atgatcccga tgatcggaaa ttttgcggcg tcggcctgaa cctgttgcca
     3421 cacctcatcc agacgaatgc gttgagcacc ccactgattc tgccaacccg gatcattcgg
     3481 cgctgttccg agcattcggt agagataatt cggctgcgcc actacgccgg gtatccggtt
     3541 caatgaatcg gcgacctgct gcggggagag ttcgttcgaa atccggtacc tgaaccaggc
     3601 acccacctgt tcagggcgtg cgctcgcgga tggtttccgg gaacgaagag ggaaaagcgg
     3661 ttcaggatcg gcaagcccga gttccgtccc gcggaaaccg ggttt
//
LOCUS       DAOTNG010000305         3703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19813_length_3703_cov_3.895559, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000305 DAOTNG010000000
VERSION     DAOTNG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19813_length_3703_cov_3.895559"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2241
                     /locus_tag="P5179_12240"
     CDS             118..2241
                     /locus_tag="P5179_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS96029.1"
                     /translation="MKQTAVSEVLERLGEPFAAGFMRDPDATMVERFANALNTWARVL
                     PLRPYDGGLLYPSGPKYWNPEGMAVGWYYVTATPLDRGVFERKLAAANPAEREALEEL
                     ERVWSAHVFPGGYTHSIPHYERVLREGLNGFDERVRKGLQTALSERDSRAGFFRAMET
                     TLDTARILHRRIVEEIERFVPGDDEQRGNRDALLAALRVVPFRPAERFFDALVATNFT
                     FYFDGCDDLGRFDQFLRPYFERSSAEGELSHDRAVELVRAQWENVNAATAWNAAIGGT
                     KPDGTDLSSELTIVCIEAARGMRRPNLALRLGPSTPDSVWEAALDTIVSGCGLPALYW
                     DPNYYRAMDASGIPLPVKERHEYAFGGCTELMVHGRSNVGSLDDDINVPGVLERSLHG
                     RLPTAADFPEFREGFRNDLAATIASIVERVNLWQQQKAEWQPQPIRSLLIDDCVDRGI
                     EYSAGGARYNWSVINVMGLANAIDSLAALRTLVFEERRFTGREIVDVLARDFDGAEEL
                     RRELENAPRYGNGDPEVDSLAHDLSQFIFTKFLKYRTWRRGADGEGGPFVPSCLMFVT
                     YVPFGRAVGATPDGRRAKEPIADSAGPYQGRDRSGPTAMMRSVTQLDQQHAPGTLVVN
                     LRLARHHFATPEQRANTKALLRTYFDMGGLQIQVNVVDQKVLRDAVAHPEKYGDLIVR
                     VGGYSEYWSNLEPALRESVLLRTEH"
     gene            2259..>2545
                     /locus_tag="P5179_12245"
     CDS             2259..>2545
                     /locus_tag="P5179_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96030.1"
                     /translation="MKHLFIDDFHVEEINNLARKLHQPEKFPRNVAIRPEYRWENCGI
                     QIRTTPAWDPEAGRFTMIYLASGESLDAEVKIGVTGAPEGGTAFYCYATSE"
     assembly_gap    2546..2645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2646..3494
                     /locus_tag="P5179_12250"
     CDS             <2646..3494
                     /locus_tag="P5179_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96031.1"
                     /translation="TTSEDFQNWSKQELVFHADQTDQENGFERLRKLIADPAYLKIVH
                     NRPEEWRTDIYNFPVFPYEDLYLALPVMHHWSGKHVPMYENVDSRKSVELASSRDLRT
                     WHRVANRAPFLEHSPVGDGSAYDTGQIVTTNGPVVRNNELWFYYVGLRHRCISIEDTL
                     NRKYLDSSAVCMARLRMDGFVSLKGGVEWGSVLTKPVNVTGDQLHINTDSWRGRVLVE
                     MLDADTMQPVPGFGKDECIPTIVNGIDETVRWKGSEDLTSLQGKTVRLRFHLWQAEMY
                     AFWFGE"
BASE COUNT          724 a         1008 c         1215 g          656 t
ORIGIN      
        1 tgcgctgcac cgtacggtcc tctgcgcttg cgagtggtgg cagggaagcg tgtacattcc
       61 tgttggattt cgctgattga agggatacgc tttttaccgg agaggatggc gtgaggattg
      121 aaacaaacgg ctgtttcgga ggtgcttgaa cggctcggcg aaccctttgc cgccgggttc
      181 atgcgcgatc ccgatgcgac tatggtggag cggttcgcaa acgccctgaa cacctgggcg
      241 cgtgtactgc cgctgcgacc ttacgacggc gggctgctgt acccgtcggg gccgaagtac
      301 tggaatccgg agggaatggc ggtcggctgg tactacgtga cggcaactcc gcttgaccgc
      361 ggggttttcg agcggaaact cgccgcagcc aaccccgctg aacgagaagc ccttgaagag
      421 ctggaacggg tctggtcggc gcacgtgttc ccgggtggat acacgcattc gattcctcac
      481 tatgagcgcg tactgcggga agggttgaac gggttcgacg aacgagttcg aaagggcctg
      541 caaacagcct tgtccgaaag ggactcccgc gcggggttct tccgtgcgat ggagaccacg
      601 ctggacaccg cgcgaatact ccaccggaga atcgttgaag agatagagcg gttcgtgccg
      661 ggagatgatg agcagagggg caatcgggat gccctgctcg cggctttgcg cgtcgttcct
      721 ttcaggccgg cggaacggtt tttcgacgcg ctcgtggcga cgaatttcac gttctatttc
      781 gacgggtgcg atgatctggg gcgattcgac cagtttctcc gcccgtattt cgaacgctcg
      841 tcggcagaag gcgagttgtc ccatgatcgc gcggtggaac tggtgcgcgc tcaatgggag
      901 aacgtgaatg ccgccaccgc gtggaacgcc gcgatcggcg gtacgaaacc cgacggcacc
      961 gacctttcca gcgaactcac catagtctgc atcgaagccg cgcgcgggat gcggcgaccc
     1021 aaccttgcgt tgcggctggg cccgtcgacg ccggactccg tgtgggaagc ggcccttgac
     1081 acgatcgtga gcggttgcgg acttcccgcg ttgtactggg acccgaatta ctacagggcg
     1141 atggatgcgt cggggattcc cctacccgta aaggaacggc atgagtatgc gtttggcggc
     1201 tgcactgaat tgatggtgca tggtcggtcg aacgtgggat cgctggacga cgacatcaac
     1261 gtgcccggcg tgcttgaacg gtctctccac gggcggctgc cgaccgcggc ggatttcccc
     1321 gagttccgcg aaggctttcg gaacgacctt gccgccacca tcgccagcat tgtggaacgg
     1381 gtgaacctct ggcaacagca aaaggcagaa tggcaaccgc agcctatacg gagccttctg
     1441 attgatgact gcgtggaccg gggcattgaa tactccgcag gcggcgcccg gtacaactgg
     1501 tcggtgatca atgttatggg cctggcgaat gcgatcgact cactcgcagc gctcagaaca
     1561 ctcgtattcg aggaacgccg cttcaccggc cgggaaatcg tcgacgtgct tgcgcgggat
     1621 ttcgatggcg cggaggagct gcggcgggag ctcgagaacg cgccgcgata cgggaatggc
     1681 gatcccgagg ttgattccct cgcgcatgat ctgtcccagt tcatcttcac gaagtttctg
     1741 aaataccgca cgtggcggcg aggtgcagac ggtgaaggcg ggccatttgt gccgtcgtgc
     1801 ctgatgtttg tgacgtacgt tccgttcggg agggcagtgg gggcaacacc ggacggccgc
     1861 cgggcaaagg agccgattgc cgactccgca gggccgtatc aggggcggga tcgctccgga
     1921 ccgacggcga tgatgcggag cgtgacacaa ctcgaccagc agcatgcgcc cgggacgctg
     1981 gtcgtgaacc tcaggctcgc acgacaccat tttgcaacgc ccgaacaaag ggcaaacacg
     2041 aaagcacttc tgcggacgta cttcgacatg ggcgggctgc aaatccaggt gaatgtcgta
     2101 gaccagaagg tgctgcggga cgcggttgcc catccggaga agtacggtga cctgatcgtg
     2161 cgcgtgggcg ggtattcgga atactggtcg aatctcgaac ccgcgttgcg ggaatcggtg
     2221 ttgttgcgta ccgagcattg atccgacgcg agggaaccat gaaacacctg ttcatagatg
     2281 attttcacgt ggaggagata aacaacctcg cccggaagct ccatcagccg gagaagttcc
     2341 cgcggaacgt cgcaatccgg ccggagtacc gctgggagaa ctgcgggatt cagattcgca
     2401 cgacgccggc gtgggacccg gaggcaggtc ggttcacgat gatctacctt gcttcggggg
     2461 agtcgctgga cgctgaggtg aagatcggcg tgaccggtgc tcccgagggt ggcacggcgt
     2521 tctactgcta tgccacctca gaggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnaccac gagcgaggat ttccagaact ggagcaaaca ggagcttgtg ttccacgccg
     2701 accagacgga ccaggaaaac ggattcgaac ggctgcggaa actgattgcc gacccggcat
     2761 acctgaagat tgtccataac cggccggagg aatggcggac ggatatttac aacttccccg
     2821 tcttcccgta cgaggacctg tatctcgcgc tgccggtgat gcaccactgg tcggggaaac
     2881 atgtgccgat gtatgaaaac gtggacagca ggaaatcggt ggaactggcg tccagccgcg
     2941 acctgcgcac gtggcaccgc gtggcgaacc gcgcaccgtt cctcgagcat tcacccgtgg
     3001 gcgacggaag tgcgtacgac acggggcaga tcgtgacgac gaacgggccg gtggtgcgga
     3061 acaacgagct ctggttctac tacgtcggcc tgcgacatcg ctgcatttcc atcgaggaca
     3121 cgctgaaccg caaatacctc gattcgagcg ctgtgtgtat ggcgaggctc cggatggatg
     3181 gcttcgtctc gctcaaaggc ggcgtcgagt ggggatccgt gttgacgaaa ccggtgaacg
     3241 tgacggggga ccagctgcac atcaacacag actcatggcg cggacgggtg ctggtggaga
     3301 tgctggacgc cgacacgatg cagccggtgc cgggattcgg gaaagacgag tgcatcccca
     3361 cgatcgtgaa cggcattgat gaaacggtgc gctggaaggg gagtgaggac cttacctcgc
     3421 tgcaagggaa aacggtgcgg ctgcggttcc acctgtggca ggcagagatg tacgcgttct
     3481 ggtttgggga gtaagaagac gaagcggcgc cgacgcggga acaacgttgg ccggtgcgat
     3541 aggatcggct cccattccct cagagcaatc aggacaggca ggaatgcctg tcccaccctc
     3601 gggagcgggg cgaagcggcg ccctcggcga gggcgccctg cctccgcgga ccacgggcgg
     3661 acacccaagc aacgccgcac gttgcaggtc cgcccctacg ttg
//
LOCUS       DAOTNG010000306         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19900_length_3689_cov_4.955421, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000306 DAOTNG010000000
VERSION     DAOTNG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19900_length_3689_cov_4.955421"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..528
                     /locus_tag="P5179_12255"
     CDS             103..528
                     /locus_tag="P5179_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRS96032.1"
                     /translation="MAFPTHYETRFDWSGSGENGLAAGEKLAPLPVGTPSAGEGRWNP
                     EAMLLSAVETCLFNTFAYIAAMSKLSFRSYHSTASGDVELVPREGYRFARITVRPVIA
                     VSGKDIARAQRILEKAHEACLISRSVNFPIDIEPTIVEE"
     gene            659..1399
                     /locus_tag="P5179_12260"
     CDS             659..1399
                     /locus_tag="P5179_12260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRS96033.1"
                     /translation="MKAAIRGFVMLVHDTAALLRVREIRTLAVGTLILLLWQYFGYPF
                     TYSEMIADKTGEWRYDMIAPNLYWTATNVILLLLLPLALETSQGKGSLRSFGLGIGDW
                     RLGCAAAVGGYVIMVPFIALAASRPDFRATYPLNQNSLLSHELFCVYEASYLLFFVGW
                     EFFFRGYWLFTLEPALGMAAVAVQTVPFALMHVGKPFPEAIGSVFAGLFLGMLALRTR
                     SIWYGVFLHAAVAGTMDIIVYLQRGIGK"
     gene            complement(1453..2652)
                     /locus_tag="P5179_12265"
     CDS             complement(1453..2652)
                     /locus_tag="P5179_12265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS96034.1"
                     /translation="MPAPIFNRRVSAIEESITLAVTAKASEMKSKGIDVVSMSAGEPD
                     FDTPDHIKYAAIKAITEGFTKYTPVGGTMELRKAVAEKFARENGLTYAANQTLVSIGG
                     KHSCYLAVAALVDDGDEVLIPAPYWVSYPDMVLLAGGKPVIMETSTATGLKITPDQLR
                     KAITPRTKMIIINSPSNPSGAVYTKDELVALGDVLKGTGVFVLSDEIYEHLIYEGEHH
                     SIAAVVPELSEQTIVANGVAKAYAMTGWRIGYAAGPKSVIGAMAKIQSQQTSNAVSIS
                     QKATLAALTGPQDHLVPWKEEYRTRRNYIVERLNAIPGVLCPTPAGAFYVFPDVSSFY
                     GKSFGGRVIEDSVALTDYLLEEFHVACVPGSGFGAPRHIRLSYATSMAAITKAMDRIE
                     KGLTALS"
     gene            2939..>3689
                     /locus_tag="P5179_12270"
     CDS             2939..>3689
                     /locus_tag="P5179_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS96035.1"
                     /translation="MRRSDFVHLHAHSAYSLLDGVSQPRKLVQRALSLGHEALALTDH
                     GAMYGTVEFYQAAKDAGIKPIIGCEVYVAARSRRDKEAQRDRKSHHLVLLARTNEGYK
                     NLIQLVTKAHMEGFYYYPRVDHELLHRFGSGLVCLSGCLSGEIPDAITSGDMDGARAL
                     IEAYHSHFGAGNFFLEVQHHPDLPSQEKVNQGVFELAEEFGIPVVATNDSHYACTTDA
                     EAQDALLCVQTSATLDQADRMSMMDGDYSLLS"
BASE COUNT          735 a         1067 c         1058 g          829 t
ORIGIN      
        1 agccatactc cccgacgttc ccccggagaa cgtggacgcg atgtttgacg aggctcgcac
       61 gtaccgcgtg aaacaccgat agaaagagaa aggcccctgt cgatggcatt ccctactcat
      121 tacgaaacac gttttgattg gagcggttcc ggggagaatg gtctcgcggc gggcgagaag
      181 ctggctccct tgccggtggg cacaccgtcc gcaggagagg gcaggtggaa ccccgaggcg
      241 atgcttcttt cggccgtaga aacgtgtctg ttcaatacgt tcgcgtacat cgcggcgatg
      301 tcgaaattga gcttccgcag ctatcactcg acggcatcag gcgatgtgga gttggtgccg
      361 cgggaaggct accgtttcgc tcgtatcact gtgcggccgg tgatcgctgt atccggcaaa
      421 gacatcgccc gtgcacagcg catccttgaa aaggcgcatg aggcgtgctt gatatcgcgg
      481 tcggtgaatt ttccgataga tatcgaaccg actatcgtag aggaatagtc ggccgcaaac
      541 ttcctgcgaa aggaatctcg cccgctccga tgtcgcggcc gcatggcaga ccccagttga
      601 cacagaagtt gcacggcacc ggtccgcgtt cgtcgaacga acgatcagaa aggcaggaat
      661 gaaggcagca atccgcggct tcgtgatgct tgtccatgat acggcggctc ttctgcgagt
      721 gcgcgaaatc cgcactttgg ccgtgggaac cctgattctt cttctctggc aatacttcgg
      781 ctacccgttc acgtattccg agatgatcgc cgacaaaacg ggggaatggc gctacgatat
      841 gatcgccccc aacctctact ggacagcgac taacgtcatc cttctccttc tcctgccgtt
      901 ggcccttgag acgtcgcaag ggaaaggcag tctgcgttcc tttgggttgg ggattggcga
      961 ctggaggctc gggtgtgccg ccgcggttgg tgggtacgtt atcatggtgc cgttcatcgc
     1021 gctggccgcc tcgcggccgg atttccgggc aacgtacccg ttgaatcaga attcgctctt
     1081 atctcacgaa ttattctgtg tttatgaagc atcctatctg ttgttcttcg tcgggtggga
     1141 gttctttttc cgtgggtact ggcttttcac actggaaccg gcacttggca tggcggcagt
     1201 tgccgtccag acagtaccat tcgcgctcat gcacgtgggc aaaccgttcc ccgaggctat
     1261 cggttccgtc tttgcaggtt tgttcctcgg gatgctcgcc ctgcggacac ggtccatctg
     1321 gtacggggtc tttctccacg ccgccgttgc cgggacgatg gacatcatcg tgtacctgca
     1381 gcgcggaatc ggaaaatgaa cgcgggagtg ccgacgtcgg cactcccgcg aagtacagcc
     1441 gcagcgctca ggtcaggaaa gggccgtcag tcctttctca attcgatcca tcgccttcgt
     1501 aatcgccgcc attgaagtgg catacgagag acggatgtgt cgtggcgcgc cgaaaccgct
     1561 gccaggaacg cacgcaacgt ggaactcctc gaggaggtaa tcagtcaacg caacgctatc
     1621 ttctataacc ctgccgccga aggatttacc gtagaacgaa gagacatccg ggaacacgta
     1681 gaacgcacct gccggtgtcg gacaaagtac gccggggatg gcattgagcc gttccacaat
     1741 gtagttgcgg cgcgttctgt attcctcttt ccaggggacg agatggtcct ggggccccgt
     1801 aagcgcggcc agagtcgcct tctgtgaaat cgagacggca ttcgaggtct gctggctctg
     1861 gattttcgcc attgcgccga taactgattt tggacccgct gcgtacccta tgcgccaccc
     1921 tgtcatggcg tacgcctttg ccacaccgtt cgccacgatc gtctgctcgc tcaactccgg
     1981 cacgactgca gcgatggaat ggtgctcacc ctcgtagatc aggtgctcgt agatttcgtc
     2041 cgaaagcacg aacacccccg tgcctttcag gacgtcaccc agtgcgacca actcatcctt
     2101 cgtgtagact gcgcccgatg ggttcgaagg gctgttgatg ataatcatct tggttcttgg
     2161 cgtgatcgcc ttccgcaact gatccggagt gatcttgagc cccgttgccg tgctcgtttc
     2221 catgattacc ggctttcctc cggcaagcag gaccatgtcg gggtagctca cccagtacgg
     2281 agccggaatc aggacctcgt cgccgtcgtc cacgagcgct gcaaccgcga gataacacga
     2341 atgcttgcct ccgatactga caagggtctg attcgcggcg tatgtcagac cgttttcgcg
     2401 ggcgaacttc tcggcaacgg ctttcctgag ctccatcgtt ccgccgaccg gggtgtattt
     2461 ggtaaaacct tctgtgatcg cctttatggc ggcatatttg atgtggtccg gcgtatcaaa
     2521 atcgggttcg ccggcgctca tggataccac atcaatgccc ttggatttca tttccgaagc
     2581 cttcgccgtt acggcaagag taatcgattc ctcgatggcc gagacgcggc ggttgaaaat
     2641 cggagctggc atgctggcat cctttcgttg ggcacagagt ggtgtcagag gacgttgcgt
     2701 gtccgaatca aaagtaccgg agggaaggag cgacaccaag ggcaggtttc ctccagtccg
     2761 gctggccgcg ctgctttgca cggctggttt cgggcatgtg gcgcgaacag gcaaatcagc
     2821 gccgagttgt cccgtggtcc cttgaccaga ggaagattca cgcggagttt tcagaaaacc
     2881 gtgtgtggag ttcctccccg ccttttgcac gaaaccatct gcccctcgga gtaacgcttt
     2941 gcggagatca gatttcgttc atcttcatgc acattcggca tacagcctcc ttgacggtgt
     3001 atcgcagccc cggaaactcg ttcaacgcgc cctctcgctc ggtcacgaag ccctcgcgct
     3061 cactgaccac ggagccatgt acggcacggt ggagttctat caggcagcca aggatgcggg
     3121 tatcaagccc attatcggat gtgaagtcta cgtggcggcc agaagcaggc gggataagga
     3181 agcgcagcgt gatcgaaaat ctcaccatct ggtattactg gcgcgaacaa acgaggggta
     3241 caagaacctc attcagcttg tgacaaaagc ccacatggag gggttttatt attacccccg
     3301 cgttgaccat gagctcctcc acagattcgg ctccggcctt gtctgtcttt ccggctgtct
     3361 ttcaggggaa atccccgatg ctattacgtc cggcgatatg gacggtgcgc gcgcgctcat
     3421 tgaagcctac cattcgcatt tcggcgcggg caacttcttc cttgaagtcc agcaccaccc
     3481 cgacctgccc tcccaggaaa aggtgaacca gggcgttttc gagctcgctg aggagttcgg
     3541 gatcccggta gtcgccacga atgacagcca ctacgcctgt acgactgatg ccgaagcaca
     3601 ggatgcgctc ctttgcgtcc agacctcggc tacactggac caggctgacc gcatgtcaat
     3661 gatggacggt gattacagcc tcctttcaa
//
LOCUS       DAOTNG010000307         3683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_19944_length_3683_cov_5.469129, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000307 DAOTNG010000000
VERSION     DAOTNG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19944_length_3683_cov_5.469129"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="P5179_12275"
     CDS             <1..1048
                     /locus_tag="P5179_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96036.1"
                     /translation="AIAGLAPNARVGGISGPLMRDAGADGSPAPDGVMGFAEPFVRFH
                     EYCSLMARSVRQFRAHPPDVSIVIDAPDFHIPFLRFVRQSRVPTVYFSPPQVWAWRRS
                     RVRAIAHLCARVVTLFDWERAYFAPPLETDRVIWEGHPLVDELPEPQEQRREGDLLIL
                     LPGSRKSEIDRLSVLFADVGRRLGVPCVFRAPTEHHAGWIRTALSRAGRAPLPVTTSP
                     LAQLLNEARACIACAGTATLEAALEGIPTVIAYRTDWLTHSVARRLVRTRWIGLPNIL
                     LRSNAYPECVQEDAEPSRIASALATVAEHPPDFWVEKAKEIRTKLGTPGVANRVVNLA
                     IETANDVPHPGIRQ"
     gene            1045..2745
                     /locus_tag="P5179_12280"
     CDS             1045..2745
                     /locus_tag="P5179_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96037.1"
                     /translation="MMNTALVLAMSFFCASRAFPQDSPVVDSAALHQSRVITEVDVVP
                     GDVVPPWWEEAVHTRRRLSRWTYAMLNALHIKTKPHVVERELLFAVGDTLRQKSLDES
                     ERNLRAYSFINEATIEAYPADSNGVRVLVRTSDNFSLAAGWIWESGGGSDRIGALVTE
                     KNLLGFGKEIAAQYERERNRSRGTTETEWLFQYKDPRLIGSRFALQVSASRAYLGNEF
                     MTALARPFTTTRTRNAGGVEFYHFTGRMRLWRDKVPVAELPVTTTDTYAWVAHAWGEA
                     SKRTKLQAALRLRNTRHTDTPLLFATWLPRDTIRVSRVAYEPSFTISRQWTSAFHKLR
                     NLDDYDTVEDVESGTNIGLTTGFGLPSQPTHNPYGLAGAFAEWCAVRGEHVTAARLQV
                     STRLADDQGSGRSAWSNREVAAYLHHYYTGLPWQTFALNVNWRSGERMDGPYQLVLGG
                     DRGLRGYSANAFEGNRRIVLNAEDRIFSPVRVLVYRLGFVVFADAGYVWQPDRRVDPR
                     DIRANIGAGFRVYNNKAATGRVSRLDIAWNVRGQRGFMISGGSEQLFDLFNRRPTPTR
                     "
     gene            complement(2742..>3683)
                     /locus_tag="P5179_12285"
     CDS             complement(2742..>3683)
                     /locus_tag="P5179_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRS96038.1"
                     /translation="WRGWPVSTTHSIVGAVAGFGFAASGADAVRWSTLARIGTSWVIS
                     PVLGALGAWAMFILVRKLVFATEHPVARARIVTPVLIGVVGSVLTLSMIYKGLKNLHL
                     NLPLTQALLLAGVIGVAAGFAGKLLLVRKLVDVGEGSREDEISAVERQFRSLQVVTAC
                     YVAFAHGSNDAANAIGPMAAVLTSAGNDFLRNFGDPTTVLLALGGFGIVLGLATYGYK
                     VIYTIGSKITEVTPSRGFVMEFSTASVVLLGSKLGLPVSTTHIIIGAVVGVGFARGMS
                     ALNTRMVGQIIWSWVVTLPSAGLLAALFFYALRTVFL"
BASE COUNT          782 a         1119 c         1082 g          700 t
ORIGIN      
        1 cgccatcgcc ggtctggctc cgaacgccag ggttggggga atctcggggc ctcttatgcg
       61 tgatgccggg gcagacggtt ccccggcgcc ggacggcgtc atgggattcg ccgagccatt
      121 tgtgcgcttt catgagtact gctccctgat ggctcgaagc gtacgtcaat tccgtgctca
      181 cccacctgac gtctccatag tcattgacgc gcccgatttc cacattccct ttctgcgctt
      241 cgtcaggcaa tcgcgcgtac cgacggtgta tttcagcccg cctcaggtgt gggcatggag
      301 aaggtcgcgc gtacgggcaa ttgctcacct ttgcgcgcga gtcgtaacgt tgttcgactg
      361 ggaacgtgcg tacttcgcac ctccattgga gaccgatcgc gtcatctggg agggacatcc
      421 cctcgtggat gagctccccg aacctcaaga acaacggcga gagggagact tgctgatttt
      481 gctccccgga agcaggaaat ccgagattga ccgcctatcc gtcctttttg ccgacgtcgg
      541 cagacgcctg ggcgtgcctt gcgtgtttcg ggcaccgacg gagcaccatg ccgggtggat
      601 ccggaccgca ctttcccgag caggtcgggc accgttgcct gtcaccactt cacctctggc
      661 gcaactcctg aacgaagccc gggcatgtat tgcctgcgcg ggaacagcca ccctcgaagc
      721 ggccttggag gggatcccga cggtgattgc gtaccgcacg gactggttga cgcacagcgt
      781 ggcgaggcgc ctcgttcgca ccagatggat agggctcccc aacattctgc tccgctcaaa
      841 cgcgtaccct gagtgtgttc aggaggatgc ggaaccctct cgaatcgcat cggcgttggc
      901 aacggtggct gagcaccctc cggatttctg ggtggaaaag gccaaagaaa tcaggacgaa
      961 actgggcaca cccggagtgg cgaaccgggt tgtcaatctg gcgatcgaga cggcgaacga
     1021 cgtcccacac cccgggattc ggcaatgatg aatacggcgc ttgtgctggc aatgtcgttt
     1081 ttctgcgcat ccagggcatt tccacaggat tccccggttg tcgattccgc ggcgcttcac
     1141 cagagccgcg tgataactga ggtggacgtc gtacccggag acgtggttcc gccgtggtgg
     1201 gaagaagcag tgcatacgcg gagacggtta tcgaggtgga cgtacgcgat gctcaacgcg
     1261 ctccacatca aaacgaaacc acacgtcgtc gagcgcgaac tcctcttcgc cgtcggcgac
     1321 accttgcggc agaaatctct ggatgagtcc gaacggaacc ttcgagcgta ctcgttcatc
     1381 aacgaagcta cgatcgaagc ctatcccgca gactcaaacg gcgtacgggt tcttgtgcgc
     1441 accagcgaca atttcagcct cgccgcaggg tggatttggg aaagcggtgg cggatcggac
     1501 agaattggag cgctggtcac agagaagaac ctgttgggtt tcgggaagga aatcgcggcg
     1561 cagtacgaac gggaacgaaa ccgttcacgc ggaacaacgg aaacggaatg gctttttcag
     1621 tacaaggacc caaggctcat aggctcgcgt ttcgcccttc aggtttctgc atcgcgcgcg
     1681 tacctcggaa acgagttcat gactgcgctg gcgcgtccat tcaccaccac gcgcacgcgc
     1741 aacgcgggcg gagttgagtt ctaccatttc accggcagga tgcgtctttg gcgcgacaag
     1801 gtgcctgtcg cggagcttcc cgttacgacg acagacacgt atgcctgggt ggcccacgcg
     1861 tggggagaag cctccaagcg caccaaattg caggccgcac tcaggttgcg gaatacgagg
     1921 cacacggaca cccccttgct cttcgccacg tggttgccca gagacacaat ccgcgtctca
     1981 cgggttgcgt atgaaccatc tttcactata agcaggcaat ggaccagcgc atttcacaaa
     2041 cttcgcaacc ttgacgatta cgacaccgtc gaggacgtcg aatcgggtac caacataggt
     2101 cttaccacag gattcggtct gccttcccaa ccgacacaca atccttacgg gctcgcaggg
     2161 gcattcgccg aatggtgcgc cgttcgcggg gaacacgtca ccgcagcacg gcttcaggtc
     2221 agcactcgcc ttgccgacga ccagggttcc ggaaggagtg catggtcaaa ccgtgaggtg
     2281 gcagcgtacc tgcatcatta ctacaccgga cttccgtggc aaacctttgc gttgaacgtg
     2341 aactggcgca gcggagagcg aatggatggt ccctaccagc ttgttctggg gggcgatcga
     2401 gggttgcgag gttacagtgc gaacgccttc gaaggtaacc gcagaatcgt cctgaatgcc
     2461 gaagaccgaa tcttttcgcc ggtgcgtgtg cttgtgtacc gactcggttt cgtggtgttc
     2521 gcggacgcag ggtacgtctg gcaacccgac agacgtgttg acccgcggga tatccgtgcg
     2581 aacatcggcg cgggattccg cgtgtacaac aacaaagccg ccacagggcg tgtgtcaagg
     2641 ctggatatcg cgtggaacgt ccgcggacaa cgcggattca tgatttcagg aggatcggaa
     2701 cagctcttcg acctgttcaa ccgcaggccg acgccgacac gctaaaggaa gaccgtgcga
     2761 agcgcgtaga agaacagggc agcgaggagc ccggcggatg gtagtgtcac cacccatgac
     2821 catatgatct gcccgaccat tctggtgttg agcgcggaca ttcctctggc aaagccgacg
     2881 ccaacgaccg cccctatgat gatgtgcgtg gtcgaaacag gcagacctag tttcgacccg
     2941 agcaacacga cggacgcggt actgaactcc atgacgaaac cgcggctggg ggttacttcg
     3001 gttatcttgc tgccgatcgt gtatatcacc ttgtaaccat acgtcgccag cccgagaaca
     3061 atgccaaacc ctcctaatgc cagaagcacg gtggtcgggt ccccgaaatt ccgcagaaaa
     3121 tcgtttccgg ccgacgtcag gacggccgcc atgggtccta tggcgtttgc cgcgtcgttt
     3181 gacccgtgcg cgaacgcgac ataacacgct gtgaccacct gcaacgaccg gaactgacgt
     3241 tccacggctg agatttcgtc ttccctgctc ccctctccca cgtcaacaag ctttcgcacc
     3301 agcaggagtt ttcccgcaaa gcccgcggcc acaccgatca caccggccag caaaagcgcc
     3361 tgcgtgagag gcagattcag atgaaggttc ttcagaccct tgtaaatcat cgaaagggtg
     3421 agcaccgaac ccaccactcc gatgagaaca ggcgtaacga ttcgtgcgcg tgccaccgga
     3481 tgttcggttg cgaataccag tttccgaacg aggatgaaca tcgcccatgc cccaagcgcc
     3541 cccaggaccg gggaaatcac ccagctcgtc ccgatcctcg cgagagtgct ccagcggact
     3601 gcatccgctc cactggcagc gaacccgaaa cctgccacag cgccgacgat cgaatgcgtg
     3661 gtcgataccg gccagccacg cca
//
LOCUS       DAOTNG010000308         3637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20276_length_3637_cov_5.332496, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000308 DAOTNG010000000
VERSION     DAOTNG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_3637_cov_5.332496"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..2677
                     /locus_tag="P5179_12290"
     CDS             17..2677
                     /locus_tag="P5179_12290"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRS96039.1"
                     /translation="MSGNTLPEALAGKPDRRVIRGKALRAVAMPLGGIGAGQIAMAGD
                     GGWRQWQIMNVPNHLGHVPHSFFAVRMRPHAVHFTERRSPEDVMGVSRVLMSSALYDS
                     PDFIPPVTSSDHIVPEESRTLLRQLPGVDEVRYVGEYPVAEVIFSDPSLPLPVTLEAY
                     SPFAPLDEEFSGLPAILCELVVTNTLESPVHVSLAGTLQNAVGWDGKSRISGTRNPGY
                     GGNENRVVRARGLTCIEMTNPSVSAESNRFGQMALSLLGGNVTWLGRWDTLEAFWADF
                     ADDGDLSCTDDAAPTSPGSTANGALCRSAWLKPGETLKATFVISWFFPNHYVNWDQSG
                     FGLKDRKSRFHIGTHYATRFQSALDVAMYVSDHAEVLRKRTFDFRNSFYDSSLPYSVL
                     DAVSSQASIIRSPTCMWLEDGTFHAFEGCHGASTGSAEDVGGCCPLNCSHVWNYEQAL
                     ARLFPRLEQAMRRTDLLHQMTEEGRIGHRTLLPFYLPRWTEPATDGQLGTVLKLYREY
                     RACGDRAFLDELYPKAKKALEWVFTKHDVDGKGILDGFQPNTYDCALHGHNTFVTGLY
                     LAALRAAEEMAKLLGDSSFGEECRARFESGKVAIDAECWNGEYYIQRPEPGKSMDMQY
                     GEGCHIDQLFGQWWAHILDLGYVFPKEHVREALRSILRYNWRDSFSGFRQSPRVFASD
                     HDSGLLICTWPHGGRPDKPTLYSDEVWPGTEYEIAGLCLFEGFAEEAFKVVEGLRNRY
                     DGVQRNPWNEVECGDHYARSMASWTVLEAACGMRYDAGRSFLGFGPNVTPERFRAPFV
                     TASGWGVFSQRITDQMATAEVIVSHGTIPLACFELDMPSKAVVVGKVLVNGKPVRCTC
                     STLDRRAHIAFGSLLELREGDTLTADVKTA"
     gene            complement(2745..>3637)
                     /locus_tag="P5179_12295"
     CDS             complement(2745..>3637)
                     /locus_tag="P5179_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS96040.1"
                     /translation="RTVALAPTHVMGSVCNMPAIMAVARKHNLKVVEDCAQSCGGKVN
                     GSYVGTFGDVGIFSISAYKIVGGGEGGLLITDDERLWDRANCLSEGGGLWRPERFAPP
                     RYEGELFCGTNYRMSELEAAVDVVQLSKMPETVARFRTVKRQITGRLKTYREIRPQTM
                     NDPDGEVGYTLRFFPETIELGQEIAKALKDAGIGAGIRGRNGRPDWHIYHDMYPITLK
                     TKSGADDCSFHCPHYAAKGGKIEYRLGDCPVADDLFDRVVNVGLNQWYTEEDCVQFAD
                     VMNSVLSHFCTEDPSAKPWR"
BASE COUNT          717 a         1043 c         1124 g          753 t
ORIGIN      
        1 aagcggagga acaacgatga gtggaaacac attgccggag gctctggctg gcaaacccga
       61 tcgtcgcgtg attcgcggca aggcattgag agcagtcgcc atgccgctgg gcgggatcgg
      121 cgcgggtcag attgctatgg ctggcgatgg tgggtggcgg caatggcaga taatgaacgt
      181 accgaaccat ctggggcacg ttccgcattc gttctttgcc gtgcgcatgc gtccccacgc
      241 agtgcatttc acggaacgcc gaagcccgga ggacgtgatg ggtgtctcac gggtgctcat
      301 gtcttccgcg ttgtacgaca gcccggattt cattcccccc gtgacaagtt ccgaccacat
      361 cgtgccggag gaatcacgga ctcttctccg gcaactgccg ggtgttgacg aggttcgtta
      421 cgtcggcgag taccccgttg cagaggtgat tttctccgac ccgtcgctgc ccctccccgt
      481 caccctcgag gcgtattccc ccttcgcgcc tctcgatgag gaattttccg gccttccggc
      541 gatcctgtgt gagctggtgg taaccaatac gctggaatca ccggtgcatg tcagcctggc
      601 ggggactctc cagaacgccg tggggtggga cggcaagagc cggatttccg gaacacggaa
      661 ccccgggtac ggcgggaatg aaaaccgggt cgtgcgagcg cggggactga cgtgcatcga
      721 gatgaccaac cccagcgtct cggcggagag caaccggttc gggcagatgg cgctttcgct
      781 actgggcggc aatgtgacgt ggctgggcag atgggacacg ttagaggcgt tctgggcgga
      841 ttttgccgac gatggtgact tgagctgtac ggacgacgct gccccgactt cccctggatc
      901 cactgccaac ggcgcattgt gtcgctccgc gtggctgaag ccaggggaaa ccctgaaggc
      961 gacgtttgtc atctcctggt tctttccgaa tcattacgtc aactgggatc agagcggttt
     1021 cgggctgaag gaccgaaaaa gccggttcca cattggcact cattacgcga cgcgtttcca
     1081 gagcgcgctc gatgtcgcca tgtacgtgag cgaccacgcg gaagtgctcc ggaaacggac
     1141 gtttgatttt cgcaattcat tctacgacag ttcattaccg tattccgtcc tcgatgctgt
     1201 ttcgtctcag gcaagcatca ttcgcagccc cacctgcatg tggctggaag acggaacctt
     1261 ccatgcgttc gaaggatgcc acggcgcaag cacgggttcc gccgaagatg ttggtggatg
     1321 ttgccccctc aattgcagtc atgtctggaa ttacgaacag gcccttgccc ggcttttccc
     1381 gcgtctggag caggccatgc gccgtacgga ccttctccac cagatgaccg aagaggggcg
     1441 aatcggccac aggacgcttt tgccgtttta cctgccgcga tggaccgaac cagcgacaga
     1501 cgggcaactc ggtactgtgc tgaagctgta ccgcgaatac cgagcctgcg gcgatcgcgc
     1561 gtttctggat gagctgtatc cgaaggcgaa aaaggcgttg gagtgggttt tcacgaagca
     1621 cgatgtcgat gggaaaggca ttctcgacgg gttccagccg aatacgtacg actgcgcact
     1681 gcatggccac aacacgtttg tcaccggtct atatctggcc gcattgcggg ccgcagaaga
     1741 gatggcgaag ctgcttggag acagctcttt cggtgaggag tgccgcgccc ggtttgaatc
     1801 agggaaggtc gcgattgatg ccgaatgctg gaacggggag tactacatac agcgtccgga
     1861 acccgggaaa agcatggaca tgcaatatgg cgagggatgc cacattgatc agttgttcgg
     1921 ccagtggtgg gcgcacatac tcgatctcgg ctacgtattc ccgaaagaac atgtccgcga
     1981 ggcgttgcgc tccatcctgc gttacaactg gcgggacagc ttttccgggt tccggcagag
     2041 cccgcgggtg tttgcctcag accatgacag cgggctgctg atctgcacct ggccacacgg
     2101 aggacgaccc gacaaaccaa cgctgtactc cgacgaggtg tggccgggga cggaatacga
     2161 gatcgcgggc ctctgtctgt tcgagggttt cgccgaagaa gccttcaaag tggtggaggg
     2221 gctgaggaac cgttacgacg gggtgcagag gaatccgtgg aacgaagtcg agtgcggtga
     2281 tcattacgcg cgcagcatgg cgagctggac ggtgctcgag gcagcgtgtg gcatgcgtta
     2341 cgatgcgggc agaagtttcc tcgggttcgg gccgaacgtt acgccggagc gtttccgcgc
     2401 acctttcgtc acggcgtcag gatggggagt gttcagtcaa cggataactg accaaatggc
     2461 tacggctgag gtgattgtct cgcacggaac gatacccctc gcgtgcttcg aactggacat
     2521 gccttcaaag gccgtggttg tcggtaaggt gctggttaat ggcaaacccg tgcgatgcac
     2581 ctgctccact ctggacaggc gagcacacat agcctttggg tcgcttttgg agttgcgcga
     2641 gggcgatacg cttacggcgg atgtcaaaac ggcgtagtga cacggagggc acaaaaccgg
     2701 agggcggacg actacccgcc ctcccggttc tcccacaggc aacttcagcg ccagggcttt
     2761 gccgacggat cctccgtgca gaaatgtgaa agcacgctgt tcataacgtc ggcgaactga
     2821 acgcagtcct cttcagtgta ccactggttc aaacccacat tcaccacgcg gtcgaaaagg
     2881 tcgtccgcta cggggcaatc tccgagccgg tactcaattt tcccgccctt tgccgcataa
     2941 tgggggcaat ggaaggaaca gtcgtcggcg ccgcttttgg ttttcagcgt gatgggatac
     3001 atgtcgtgat agatgtgcca gtccggccgc ccgttccgtc cccggatacc ggcgccgatt
     3061 ccggcgtctt tgagggcctt cgcaatttcc tgccccagtt cgattgtttc ggggaagaaa
     3121 cggagcgtgt agccgacctc accgtcggga tcgttcatcg tctgcggacg aatttcccgg
     3181 tacgttttca gtctgccggt gatctggcgt ttcacggtcc ggaaacgcgc caccgtttcc
     3241 ggcatcttcg atagttgcac gacatccacc gcagcctcaa gctcggacat gcggtagttg
     3301 gtgccacaga acagctctcc ctcataccgt ggaggcgcga aacgttccgg acgccacaat
     3361 ccgccgccct cggagagaca gttggcacga tcccagaggc gctcgtcgtc ggtgataagc
     3421 agccctccct ctcctccgcc cacgatcttg tatgcagaga tactaaagat tcctacgtca
     3481 ccgaaagtgc ccacgtagct tccgttcact ttgccgccac aggattgggc gcagtcttcc
     3541 accaccttca ggttgtgctt acgcgcaact gccatgattg cgggcatgtt acacacgctc
     3601 cccatcacgt gggtcggtgc gagggcaacg gtgcgcg
//
LOCUS       DAOTNG010000309         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20416_length_3615_cov_4.788202, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000309 DAOTNG010000000
VERSION     DAOTNG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20416_length_3615_cov_4.788202"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="P5179_12300"
     CDS             complement(<1..777)
                     /locus_tag="P5179_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRS96041.1"
                     /translation="MERTGTGVCAPKGFSATGVACGLKAHGRRDLALIVSRREASAAA
                     LFTTNLVCGAPISVSRQHITGGVARAIVVNAGCANACTGARGVADAERMCSLVASGLN
                     CPPRQVLVASTGVIGRLLPMDRIERGIEAALDDVSEDGGSAAADAIITTDTFPKTVKR
                     VLSIGGHTVTIGGMAKGAGMIAPKLATMLAFITTDALLTPEDAHSLLRNAAEASFNRL
                     TIDGDTSTSDTLTLLANGESGAPALKPGTDDYVKFGEQLTD"
     gene            967..1419
                     /locus_tag="P5179_12305"
     CDS             967..1419
                     /locus_tag="P5179_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96042.1"
                     /translation="MRADLESETRQADEPFLARFDPKPDESALWTLVSGIGQFVVAGW
                     CITIAGLMIVVGYAGMHGRGSVLASAAAILMTLFVLVGVALAAIGVRFLARSARAFWV
                     AMRKALFVVSFNQAADKRRALHAIVGDPLLEPYARYLLQRGDLKVPPL"
     gene            1781..3313
                     /locus_tag="P5179_12310"
     CDS             1781..3313
                     /locus_tag="P5179_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96043.1"
                     /translation="MASRTVILINPDREKPLIAPLGLDYLAATLRHAGYEPELLDLAF
                     ESDWYAATNQAVSKRDLLAVLILIRHLDDGLYNSQTFFLPKSRTLIEYLRSKTKAPIG
                     VIGGAYSLVPEAILRFCRADFGIANDPEVALPMLLEAIESGDGYEGVPNVGWIEGKAF
                     RRNPIKTARVDGEWLAERSLIDNVRYFNESGVTRFETKRGCAHNEVYSTDPITRGNSV
                     RLRPAEHVAEEVERLAARGIYSLYADDTRFNDPREHALEVCRAMAERKLRDRVRWYAT
                     CTPKGLDAELAEAMEAAGCQGVELFTDSGDADQLRRMKHTHTPEDILTAIESCKRYGI
                     VVISEVQIGGPGESRKSIERTLGLMKASGPDMINIHYGLRVYPNTPLGNAVQTVTPLR
                     NNFHLRGAIYNNESLLRPVFYIESLLGRGVEEFIEELIDHDPRYLFAFRKEVDKTYNY
                     NDPTILTEAILHEGHRGAQWDIWRRLIYRLPPLSLNGDRPSRGKRRPRRVIQSAEAGV
                     AV"
     gene            3405..>3615
                     /locus_tag="P5179_12315"
     CDS             3405..>3615
                     /locus_tag="P5179_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRS96044.1"
                     /translation="MKLGTCAYSYRDLLTKGKMTLIELLDTVRDLGFDGIEWTAYYFP
                     ETTKDYLLNIKRETFVRGLDISATAV"
BASE COUNT          743 a         1038 c         1051 g          783 t
ORIGIN      
        1 gtcagtcagt tgttccccaa atttcacgta gtcatccgtt cccggtttca acgctggcgc
       61 gccggattcc ccattggcca gcaaggtgag cgtatcgctg gtggaagtgt ccccgtcaat
      121 ggtcaggcgg ttgaacgagg cctctgcagc gttccgcaac aacgagtggg catcttcggg
      181 ggtgagcagg gcgtccgtcg taatgaaggc aagcatggtt gcgagttttg gggctatcat
      241 tcccgcgcct ttggccatgc cgcctattgt caccgtgtgc ccgccgatgg acagaacacg
      301 cttgactgtt ttcgggaaag tatctgttgt gatgatggca tcggcagccg ctgagccccc
      361 atcttccgat acgtcatcca acgcggcttc gatcccgcgc tcgatcctgt ccatggggag
      421 tagtctgccg atcactcccg tggaggcaac gagcacctgc cgaggtggac agttgagacc
      481 ggaggccaca agcgaacaca ttctctccgc atccgccacg ccgcgcgctc cggtgcacgc
      541 gttcgcgcac ccggcgttga ccacgatcgc acgcgctacc ccaccggtta tatgctgtcg
      601 tgacaccgag atgggcgcgc cacacacaag attcgtcgtg aaaagcgccg cggcggaggc
      661 ttctcttctg ctcacgatga gtgcgaggtc ccggcgcccg tgtgctttca aaccgcaggc
      721 cacacccgtg gcggagaatc ccttcggcgc gcaaacccct gtccctgttc tttccatcct
      781 gtaacgtatc cttccgtgca ctaacccact ggtgaacggc gaaaacagcg catccggcgg
      841 agccggttgc agtcaaggct tcctttcatg gcgcggaaga agcccgtaat taagctatat
      901 tctgaacgac agtaacaccc acccccggac gccatttctg tcgtggcaac cggaacggag
      961 aggaaagtgc gcgcagacct cgaatcggaa accagacaag cagatgagcc ttttctggcg
     1021 aggttcgatc ccaagccgga cgaaagtgcg ctctggactc tggttagcgg tatcgggcag
     1081 tttgtggtgg ccggctggtg catcaccatc gccggcctga tgattgtggt cggctatgcc
     1141 ggcatgcatg ggcggggttc cgtgctggcg agcgcggcgg ccattctgat gactctgttt
     1201 gtgctggtgg gagttgcgct ggcggcgatc ggcgtgcgtt ttctcgcccg ttccgcaagg
     1261 gcattctggg tagcaatgcg taaggcgctg tttgtcgtgt cgttcaacca ggcagcagac
     1321 aagcgaaggg cattgcacgc gattgtcggc gatcctcttc tcgaaccgta cgctcggtat
     1381 ctgcttcaac ggggcgatct caaggtgccg ccgctgtgaa gcgcacgttt tccccgcaag
     1441 ttttgttccc gctggatgac actccgactg aaatctacta attcattgac tgtcagcatc
     1501 ttacatcatg tttttgtcaa tcaattgtcc aatgtgttat cttttgtact accaaatttg
     1561 aaggtttgac tccttcggat tgcgttcgcc ccaaaccagg gtccggacgc gctgatatgc
     1621 ggacgaagag ctgcggtttg ccgccttttg ccacactcgg agcatgcgta tcgcttcgga
     1681 tcccatctcc cctcccagtt tttgtcttcg ttatcggcgc gtaacccacg aggatatgag
     1741 acggcgaggg gaattcaagt agtcttgcag gaggactgct gtggcttcac gaacggttat
     1801 tctgataaat cccgaccgcg aaaaacccct cattgctcct ctcgggcttg attacctcgc
     1861 ggcgacgttg cgccacgcag ggtatgaacc ggagcttctc gatcttgcgt tcgagagtga
     1921 ttggtacgcc gcaacgaatc aggcggtatc gaagcgcgat cttctggccg ttttgatatt
     1981 gattcgtcac cttgacgacg gtctgtacaa cagccagacg tttttcctcc cgaagtcccg
     2041 gactctgatc gaatatctcc ggagcaaaac aaaggcgcct atcggcgtta tcggcggggc
     2101 gtactctctc gtgccggaag ccatcctgcg attctgcagg gcggatttcg ggatcgcgaa
     2161 tgacccggaa gttgcccttc ccatgcttct cgaggcgatc gaatcaggcg atgggtacga
     2221 aggtgttccg aacgtggggt ggatcgaagg gaaagcgttc cggcggaacc cgatcaagac
     2281 ggcgcgcgtc gacggggaat ggcttgcaga acgaagtctc attgacaacg ttcggtattt
     2341 caatgaaagc ggagtaaccc ggtttgagac caagagaggc tgcgcgcaca acgaagtcta
     2401 ttcaaccgac ccgataacgc gaggtaacag cgttcggctc cgtccggcgg agcacgttgc
     2461 cgaggaggtg gaacggctgg ctgcccgggg catttacagc ctgtatgctg atgacacgcg
     2521 attcaacgac ccacgtgagc acgcgctgga ggtgtgccgt gcgatggccg agcggaagct
     2581 cagggatcgg gtacggtggt acgccacatg cacacccaag ggtctcgatg ccgaactcgc
     2641 ggaagcgatg gaagccgcgg ggtgccaagg tgtcgagctg ttcaccgatt ccggcgacgc
     2701 ggaccagctc cgacggatga agcacacgca tactccggaa gacatcctca ccgcgatcga
     2761 gtcttgcaaa cggtacggga tcgtcgtaat cagcgaggtt cagataggcg gccccggcga
     2821 gtcccgcaaa tcgatagaac ggactctggg gctcatgaaa gcttccgggc ccgacatgat
     2881 caatatccac tacgggcttc gggtgtatcc gaacacgccg ctcggcaacg cagttcaaac
     2941 ggttacgcct ttgaggaaca acttccacct ccggggagcg atttacaaca acgaatcgct
     3001 tttgcgtcct gtgttttaca tcgaatccct gctcgggcgc ggagtggagg aattcataga
     3061 ggaattgatc gaccacgacc cacggtatct ttttgcattt cgcaaagagg ttgacaagac
     3121 gtacaattac aatgacccca caattctgac cgaggcaatc cttcacgagg gccatcgcgg
     3181 ggcgcaatgg gatatctggc gccgtctgat ttaccgtctt ccgccgcttt cactgaacgg
     3241 tgaccgtccc tcgcgaggaa agcgacggcc gcgaagggtc attcaaagcg ccgaagcggg
     3301 cgttgctgtt tgacggtcgt tcacgcgaac ttgtacagac gaaagggggc cccgatcccc
     3361 ctttcgtctt ttccggcata ccccgaaaat gaggctctga tccgatgaaa ctcggtacct
     3421 gtgcgtattc ctaccgcgac ctgctcacca agggcaagat gacgcttatc gaattgctgg
     3481 acaccgtgcg cgatctcgga ttcgatggca ttgaatggac cgcgtactat ttccccgaaa
     3541 cgacaaaaga ctatctcctc aacatcaaac gggagacatt cgtgcgcggg ttggacattt
     3601 ccgccacggc cgtcg
//
LOCUS       DAOTNG010000310         3585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20633_length_3585_cov_4.655807, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000310 DAOTNG010000000
VERSION     DAOTNG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20633_length_3585_cov_4.655807"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3585
                     /locus_tag="P5179_12320"
     CDS             <1..>3585
                     /locus_tag="P5179_12320"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS96045.1"
                     /translation="LRFARSVLREGGGIRINPSQITITPKPLTAGALQLGEDNFTLTQ
                     QVMLKPNMTYQVSVWDGPLPASALFTTGSAFPAGSISGQISISSSSSVSAVDHAYVAL
                     LTSPPSTTNMDSLPVFRIVGTVDGKFRFTNLPDSTYYVFAAGYYGGGEGYDQQISTTL
                     VPAPLKIAGGNAITGANYALGGSSTASPPRVVSFNVAVVQNRQPIAVFFPTVSARVTD
                     ANGDNTIASVFVRKPDGSVVTVPRVSDGVYEGVLDPLYTFAGGTYRLWAVDNTGLESA
                     VATDSISPPSLPEPAIISPANNASNVSLTPLLDWSDVVGAAGYVVNVSTENPISFSDP
                     AGAVARFLDPAKTVVNFADAPVTVSELRIPSGRLKPNTTYYWGVGAIDKMVDHDHVSL
                     SLLPSFTTGTGTAVADTLPPVFTKTPEVTSVDSSSIALGWVTDEASDTRVRFGRSAGV
                     YTDSLAEPTMTKVHAIRITGLAPATEYFLEVSSRDHAGNVARMPLQRSVITRDRADRT
                     APNFVAGPTTERVDVNSVTIYWANDEPTTAVVNLVGVGKDTTVYDTRLVREHRLDILG
                     LSPTTSYAYSVSVIDAAGNGPTIRPGQAFVTKGLPDTNPPRALTGPVVTANERDAFIA
                     WVADELHTARVEIALAGAQGVMFSETFVDIPAVSQLARLTGLAPGTEYRAIVEMTDLA
                     RNTTKTRPITFWTKSVPDTIPPRILRFPTVVYRADTRAIVEWETDEMADSYLELYQGA
                     RLVNTFNRGDLVRVHRFVLTNLQAGTAYSFRVINADAARNQVVWPLTPPSAKPTKEAG
                     GRTGSEFTTAVLPDQTAPQVVSGPTVLSATATTVTVGWNTDENANSVVRFGEGSAGRI
                     AREGATVEFTDAVTITENVISHQVTLTGLKPGTAYTYQIGSTDPSGNGETTSNPQTVY
                     TLHEEDLAAPLILGGPAVIGRTDSRITVKWTTDEPSNSKLLFRRVGSADQPTEVVVPD
                     NVIEHVVTLTNLLPSQSYEITVQSSDLVGNGPASASVTGATEAAADTKAPSFTGGPTV
                     SADATQALISWTTDEPSDTRVEYGTTVALGLVVNLATFSTNHSVTLTNLTPATQYYYR
                     ISSGDASGNIADSTSGSLVWTTLASADVTPPEKVTGVTSINGAYSVRLSWAANTDADL
                     DVYVIERAIGAGGFAKIADQKVTTFTDQAVLTGTTYRYRLLAQDRSANR"
BASE COUNT          715 a         1072 c         1097 g          701 t
ORIGIN      
        1 tctgcggttc gccagatcag tgctgcgcga gggtggtggt atccggatca atccgagcca
       61 gatcacgatc acgcccaaac cactgacggc tggcgctctc cagttgggtg aggacaactt
      121 cacccttacc cagcaggtga tgctcaagcc gaacatgacc tatcaggtgt ccgtctggga
      181 tgggcctttg cccgcgtcgg cgctcttcac caccggtagt gcattccctg ccgggtcaat
      241 cagcgggcag atttccattt cgtcgtcctc ttccgtttcg gcggtggatc acgcgtacgt
      301 ggctctgtta accagtccgc ccagtacgac gaacatggat tcacttccgg tgttccgtat
      361 tgttggcacc gtggacggga aattcaggtt cacgaacctt ccggacagca cctattacgt
      421 gtttgccgct gggtactacg gtggcggaga aggttacgat caacagatca gcacgacgct
      481 tgttcccgct ccgctcaaaa tcgcgggcgg caacgcgatc accggtgcga actacgctct
      541 tggtggttcc tccaccgcga gtccgccacg cgttgtgagt ttcaacgtgg cggtggtaca
      601 aaatcggcag ccgatagcgg ttttctttcc cactgtttcc gcgcgggtga ccgatgcaaa
      661 tggtgacaac acgattgcgt cggtgtttgt gaggaaaccg gatggcagcg tggtgacggt
      721 tccgcgcgtg agtgacggcg tgtacgaagg agtactcgat ccgctgtaca cgtttgcggg
      781 aggtacctat cgtctctggg cggttgacaa caccggcctg gaatccgcgg ttgcgactga
      841 ttcgatctcg cctcccagtc tccccgaacc ggcgatcatt tcgcctgcga acaacgccag
      901 caatgtgtcg ttgaccccgt tactcgactg gagtgacgtg gtgggggcgg ccgggtacgt
      961 ggtgaacgtc agcacggaga acccgatcag cttcagcgat cctgccgggg cggtggcccg
     1021 gttccttgat ccggcgaaaa cggtagtgaa cttcgcggat gctccggtca ccgtgagcga
     1081 actacgcatc ccgtcagggc gtctcaagcc caacacgacc tattactggg gcgtcggcgc
     1141 tatcgacaag atggtggatc acgaccatgt gtcgttgagc ctcttaccat ccttcacaac
     1201 ggggacggga acagcggttg ccgacactct gcctcccgta ttcacaaaaa cgcctgaggt
     1261 tacgtcggtg gattccagtt cgattgccct cggctgggtc accgacgaag cgtccgacac
     1321 acgtgttcgt ttcgggcgga gcgcgggagt gtacaccgac agccttgccg aacccacgat
     1381 gacgaaggtg cacgcgatca ggattaccgg tctggcgccg gccacggaat acttcctcga
     1441 agtatcttcc cgtgaccatg cggggaacgt cgcgcgcatg ccgctgcaac gttcggtgat
     1501 caccagggac cgcgcggatc gcactgctcc caatttcgtc gcgggtccga cgaccgaacg
     1561 tgtcgacgtg aacagtgtga cgatctactg ggcaaacgac gaaccgacca ccgcggtggt
     1621 gaacctggtg ggcgtcggca aggatacgac ggtctacgac acacggctcg tccgcgagca
     1681 ccggttggac atcctcggcc tgtcgcctac gacgtcgtac gcgtacagcg tctcggtcat
     1741 agacgcggcg ggaaatggtc ccactatccg gcctggacag gcatttgtca cgaaaggcct
     1801 tcctgacacc aatccacctc gggctctcac cggaccggta gtcactgcga acgaacggga
     1861 tgcgttcatt gcgtgggtcg cggatgagtt gcatacggcg cgcgtggaga ttgcgcttgc
     1921 gggtgctcag ggagtcatgt tctcggagac gttcgtagac ataccggcgg tttcgcaact
     1981 ggctcgtttg accgggctcg ctcctggtac cgagtaccgt gcgatcgtgg aaatgactga
     2041 tctggcgcga aacacgacaa aaacacgccc gatcaccttc tggacgaagt ctgttccaga
     2101 cacgataccc ccacgtatcc tgcgtttccc gacggtggtg taccgcgccg acacgcgtgc
     2161 aatcgtcgaa tgggaaaccg acgagatggc cgacagctat ctcgagctgt atcagggagc
     2221 acgacttgtc aatacgttca accgtggcga tctcgtccgc gttcaccgtt tcgtattgac
     2281 aaaccttcag gcaggcactg cctattcttt ccgggtgatc aatgccgatg ccgcccggaa
     2341 ccaggtggtg tggccgctca ctccaccgtc cgccaaacca acaaaagaag cgggcgggcg
     2401 aaccggatcg gagtttacga cggccgtgtt gccggaccag accgcgccgc aggtggtatc
     2461 ggggccgacg gttctctcgg ccaccgcaac gacggtgacg gtcgggtgga acacggatga
     2521 gaacgccaac agcgtggtac ggttcggaga gggatcggcc ggaagaattg cccgcgaagg
     2581 tgccacggtg gagttcacgg acgccgttac tatcacggaa aatgtgattt cgcaccaggt
     2641 tacgctgacc ggactcaaac cgggaaccgc ctacacgtac cagatcgggt ccacggaccc
     2701 gtcggggaac ggggaaacca cgagcaatcc gcagaccgtg tacacgctcc acgaagaaga
     2761 cctcgcggcg cctctgattc tcggcggccc cgcggtgatc ggccggacgg attcacgtat
     2821 cacggtgaag tggacgacgg atgaaccttc caacagcaaa ctgttgttca ggcgcgtggg
     2881 atcggcggat cagcctaccg aggttgtggt tcctgacaac gtgatcgaac acgtggtgac
     2941 gttgacgaac cttctgccat cgcaatcgta tgagatcacc gtgcagtcct ccgacctggt
     3001 gggcaacggc ccggcttcgg cctccgtaac tggcgcgacc gaagcggcgg ccgacacgaa
     3061 agcgccttcg ttcacgggtg gcccgacggt ttcggcggat gcaacacagg cgctgatttc
     3121 gtggacgacc gacgaaccga gcgacacgcg ggtggagtac ggcacgacgg tcgcgcttgg
     3181 ccttgttgtg aacctcgcga cgttcagcac caaccactcg gtaacattga cgaatctcac
     3241 gcctgcgacg cagtactact accggatttc ctccggcgat gcgagcggga atatcgcgga
     3301 ttccacgagc ggatcgctgg tctggacgac gcttgccagt gcggacgtga cgccgccgga
     3361 aaaggtgaca ggagtcacgt caatcaacgg tgcgtattcg gttcgcctct cgtgggcggc
     3421 caatacggat gcggatcttg acgtttacgt catagaacga gcaatcggtg ccggtggttt
     3481 cgccaaaatc gccgatcaga aggtcaccac gttcactgac caggcggtgt tgaccggcac
     3541 tacctaccgg tatcggctcc tggcacagga ccgttccgcc aaccg
//
LOCUS       DAOTNG010000311         3560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20796_length_3560_cov_5.799429, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000311 DAOTNG010000000
VERSION     DAOTNG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20796_length_3560_cov_5.799429"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1316)
                     /locus_tag="P5179_12325"
     CDS             complement(66..1316)
                     /locus_tag="P5179_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96046.1"
                     /translation="MNGVPIPDEPVRVALIGAGSRARSIYRPIMPSMSDWMRVVAVCD
                     PVREHADALAASYGVPAFYDIRELVRSRPMEAAVVVTPVESHHSISCFLSAHGIHNLV
                     ETSWCNTVTQGRQMIEAARRGKVLVRVAENFFRFPIDRIVGKLAAAEVVGPVHRVVSY
                     ADHTGYHNNSRWVAFFGAHPEWVQSVEHTMPTMAFHESPERFHDSETYRARFFQFPGN
                     GFVMDHLANVKGFLGRYPRPGYTEWQGERGTIVHFARRGWQGEGEIRLCTDSEFGRRG
                     IADTTIPIVTECPDHRWTRTYADLRPVGGGVVEYVNPFRPEEIPQHASDWYGCAIMDH
                     LADFACAVRGIRASEFDEQDALMSMMMEVGAKESALKEGARVRLPIDGEPESDGRIHA
                     ALRARFGVDPLDIEAMLAISYPKP"
     gene            complement(1321..2709)
                     /locus_tag="P5179_12330"
     CDS             complement(1321..2709)
                     /locus_tag="P5179_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96047.1"
                     /translation="MPVPTNLPDNIRTVLNACPPLRNQRGSRELVYQYSIGSLSALND
                     EEAEWTIRELAARGIGVISMWQTGANQDAGIAEALRIGRIQKKLGLTVACDANALLYS
                     FYDKTAATAHVDEAGQPFFDESFSGPKMGCPFALEHRMPVILGRVAAYVEAYHQAGVP
                     IGIVTADWEIDGPHEWNDAWRSSRQCARCRENLPNIDDFSAFQARMRQMRSRLQRECY
                     ADPILARNPKALVTNYAVYPNDGWRYWYDYFEHPQPELPHKRDQNDLHRPWYNDFFET
                     HFTLAMPVVYTWYRIYDAYPEFTNPDYRWFYNMLLVGTNAGKSTPSGIPIATFVHWHT
                     TAPPADSPAVPQMSAEAYTELLWHLLLRGHDIFYSWTPMDELAEEIALVQRVYDGSLL
                     YNDWLLNGTPICFDVPPQQGPVISGVRVGNQVLLRRTDFGDVREPVTITVDGRRLVVP
                     VSPGRMQILDLE"
     gene            complement(2888..>3560)
                     /locus_tag="P5179_12335"
     CDS             complement(2888..>3560)
                     /locus_tag="P5179_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS96048.1"
                     /translation="VRSDSVPLLGGPRYRSMTLRGEAEYDTRDVRENPTTGMRYALNW
                     RYDMRRNRVYDSDFLAYYGGSAWLRNERVSVVKVDLEHFLPVRRAIVAAVGWHGGQIS
                     STSGRTTFSAADELRLGGALTLRGYRQDQFAGDRIIWGNHELRYLLGGAARVFVFVDA
                     GYVRTRRIDAQSGMTASAETYPVGYGGGLRARTAAGNLGIDFGWGRHDTFSRGKVHAR
                     IETTF"
BASE COUNT          675 a         1140 c         1074 g          671 t
ORIGIN      
        1 acccgccctg gagggacccg ctctgtcggg tccaacaggt gtgcgcggga acctcacttt
       61 gccacctacg gcttcgggta gctgatcgcc agcatggcct cgatgtcaag cggatcaacg
      121 ccgaaccgtg cgcgcagcgc cgcgtgaatc cgcccatcgc tctccggttc cccgtcaatc
      181 ggcaggcgca cgcgcgcgcc ttctttcagc gccgattcct tcgcacctac ttccatcatc
      241 atggacataa gcgcgtcctg ttcgtcgaat tcgctcgccc ggatcccccg caccgcgcag
      301 gcgaaatcgg cgagatgatc catgatggcg cagccgtacc agtcgctggc gtgctgcggt
      361 atctcctccg ggcggaaggg gttcacgtat tccaccacgc cgccgccgac gggtctcagg
      421 tccgcgtatg tgcgtgtcca tcggtggtcg gggcactccg tgacaatcgg gatcgtggtg
      481 tcggcgatgc cgcgccggcc aaactcggaa tcggtgcaga gccggatctc cccctctccc
      541 tgccagcccc gccgcgcgaa atggacgatg gtgccgcgct cgccctgcca ttcggtgtag
      601 ccggggcgcg gatagcgtcc gagaaagccc ttcacgttgg ccaggtgatc catgacgaag
      661 ccgttgcccg ggaactggaa aaaacgcgcc cgataggttt cgctgtcgtg gaaccgctcc
      721 ggcgattcgt gaaacgccat ggtgggcatg gtgtgctcga ccgattgcac ccactcggga
      781 tgggccccga agaaggcgac ccagcgcgag ttgttgtggt aaccggtgtg gtcggcgtag
      841 gaaaccaccc ggtgcaccgg gccgaccact tccgcggcgg cgagcttccc gacgatgcgg
      901 tctatcggga agcggaagaa gttctcggcc acgcgcacga gcaccttgcc ccggcgcgcc
      961 gcttcgatca tctgccgccc ctgcgtgacg gtgttacacc agcttgtttc cacgaggttg
     1021 tgaatgccgt gcgcggaaag gaagcaggag atggagtggt gcgactccac cggagtaacc
     1081 accactgccg cttccatcgg gcgtgaacgc accagctcgc ggatgtcgta gaacgccggc
     1141 acaccgtacg acgcggcgag cgcatccgcg tgctcacgca ccgggtcgca caccgccacg
     1201 acgcgcatcc agtcgctcat ggacggcatg atcgggcggt aaattgagcg tgctcgcgag
     1261 cccgcgccga tcagcgcaac gcggaccggt tcgtcgggaa tgggaacgcc gttcacgcgt
     1321 ctactcgagg tcgaggatct gcatgcgacc cggcgagaca ggaacgacga gtctgcggcc
     1381 atccacggtg atcgtcacgg gttcccgcac atcaccgaaa tccgttcgcc gcaggagcac
     1441 ctggttcccc acacgaactc ccgaaatgac cggtccctgc tgcggtggaa catcgaagca
     1501 gatcggggtg ccgttgagca gccagtcatt gtagagcagg gagccgtcgt acacgcgctg
     1561 gacaagggcg atttcctctg ccagctcgtc catcggcgtc catgagtaga agatgtcgtg
     1621 tccccgcagc aggaggtgcc agagcagttc cgtatacgct tccgcgctca tctgcggcac
     1681 agccgggctg tctgccggcg gagctgtggt atgccagtga acgaacgtgg cgatcggaat
     1741 gcctgacggg gtggatttgc ctgcgttcgt gcctaccaga agcatattgt agaaccagcg
     1801 gtaatcggga ttggtgaact ccgggtacgc gtcataaatg cggtaccagg tatacactac
     1861 cggcatcgca agagtgaaat gcgtctcgaa gaaatcgttg taccacggac ggtgaaggtc
     1921 gttctggtct cttttgtggg gaagctccgg ctgtggatgc tcgaaatagt cgtaccagta
     1981 ccgccacccg tcgtttgggt acaccgcgta attcgtcaca agcgccttcg ggtttcgcgc
     2041 aaggatcggg tccgcgtaac actctcgctg gagcctgctg cgcatctggc gcatgcgcgc
     2101 ctgaaacgcg gaaaaatcgt ctatgttcgg caggttctcg cggcaccgcg cgcactgtct
     2161 ggaagacctc cacgcatcgt tccactcgtg cgggccatca atctcccagt ctgcagtcac
     2221 gatcccgatg ggaacgccgg cttgatggta tgcctccacg tacgccgcaa cgcgacccag
     2281 tatgacgggc attcggtgtt ccagtgcgaa gggacacccc attttcggcc cgctgaacga
     2341 ttcatcgaag aatggctgtc ccgcctcgtc cacgtgcgcg gtggcggctg ttttgtcgta
     2401 aaagctgtac agcagagcgt ttgcatcgca agccaccgta agcccgagtt ttttctgtat
     2461 cctcccgatc cgcaacgctt ctgcgattcc ggcatcctgg ttcgccccgg tctgccacat
     2521 gctgatgacg ccgattcccc gcgctgcaag ttcacggatc gtccattcgg cttcttcgtc
     2581 gttcaacgcg ctcaggctgc cgatggaata ctgatacaca agctcccgcg agcccctctg
     2641 gttcctcagc ggagggcagg cgttgaggac ggtgcggatg ttgtccggaa gattcgtggg
     2701 aacaggcatg ggtaactccc ttgaaaacgc ggtggaggcg aacgccaaca aaacacctgt
     2761 cagatggaac aagattatcg acccttcaga aacacgcggg caatctgcgc cacatgcggg
     2821 aacacgtatc catgatactg gaagacgtcg gaaatgcgca acggaaactg atcgacgcat
     2881 gaatcggtca gaacgtagtc tcgatccggg cgtgcacttt tccccgtgag aatgtgtcgt
     2941 gccgccccca tccgaaatca attccgagat tccctgcggc ggtacgggca cgaagccctc
     3001 ccccgtatcc gacagggtat gtctctgccg atgcggtcat gccgctctgc gcgtctatcc
     3061 ggcgtgtgcg cacgtacccg gcatcaacga acacgaacac gcgcgcggcc cctccaagaa
     3121 ggtaccgtaa ctcgtgattg ccccagatga tccgatcacc ggcgaactgg tcctgtcggt
     3181 acccgcgcaa tgtcagagca cctcccaggc gcagttcgtc cgcggcggag aatgtggtcc
     3241 gcccgctggt gctggaaatc tgccctccgt gccagccaac cgcggcgaca atggcgcgcc
     3301 gcaccggcag gaaatgctca aggtcaacct tcaccacgga cactcgctcg ttccgtaacc
     3361 aggcggatcc gccgtagtac gcgaggaaat cggagtcgta cacccgattc ctccgcatat
     3421 cgtaccgcca gttcaacgcg tatcgcatgc cggtcgtggg gttctcccga acgtcccgcg
     3481 tgtcgtattc cgcttcccct cgcaacgtca tgctccggta ccgcggacca ccgagaaggg
     3541 gtactgagtc gctacggacg
//
LOCUS       DAOTNG010000312         3554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20837_length_3554_cov_5.232066, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000312 DAOTNG010000000
VERSION     DAOTNG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20837_length_3554_cov_5.232066"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="groEL"
                     /locus_tag="P5179_12340"
     CDS             <1..1090
                     /gene="groEL"
                     /locus_tag="P5179_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRS96049.1"
                     /translation="ETTLDVVEGMQFDRGYISPHFVTDPDAMEAVLEDALVLIHDKKI
                     SSINDLLPLLEKVGQAGKELLVIAEDVEGEALATLVVNKLRGVLKIAAVKAPGFGDRR
                     KAMLEDIAVLTGGKVISEEAGLKLENTRIEDLGRAKRIVVDKDNTTIVEGAGDHAAIQ
                     GRVKQIRKQIEDTTSDYDREKLQERLAKLAGGVAVVHVGAATETALKEKKARVEDALH
                     ATRAAVEEGIVPGGGVALLRAMKALDSLKLEGDLKVGVDIVRRAIEEPCRWIVQNAGR
                     EGTVVVQNILANKDKNYGFNAQTEEYGDLVKQGVVDPTKVVRTALENAASVSALLLTT
                     EAMVAEKPEPKKPAAPAAGGHHHPHGGF"
     gene            complement(1163..1933)
                     /locus_tag="P5179_12345"
     CDS             complement(1163..1933)
                     /locus_tag="P5179_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96050.1"
                     /translation="MFAETQAVRAGGSRLRAVVLATFILTGTAPAALVDPPRLVVPFS
                     VSGDSVTVVPVLITSSHSFGADLAFLIDTTVARPDRDFIRAHAFESEPSSLCLWNAVG
                     DSVFVSVSSPHPRDLSDVPLVELAFRGKRDVPSGSATALTLLPYPFSTFNEHGLSVAA
                     SDKSTTPHFSLSAAPNPFNSATAIRTSVPCTVVARLTVYATDGRAVRTLTPTAAAGMH
                     TFVWDGRDEAGRRVASGVYLCRLEASHQTSVIRVTVIR"
     gene            complement(1923..>3554)
                     /locus_tag="P5179_12350"
     CDS             complement(1923..>3554)
                     /locus_tag="P5179_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96051.1"
                     /translation="AYIYGSTVLLKHTLIAGNSAGGEGGGLYVFGQSAVNVTQCTLVD
                     NGAGEVRTGAIGCGNGAEITLNSSIVWNSGGIPAPDTCHVTYTAIEAGYPGEGNTTAD
                     PLFVNAAAGDYRLGSGSPCINTADPALPADPDGTRADMGAYAYVNPSAELILPQVIAH
                     AGTTVPFPITARVADAFGADMAFLLDAGLVESIRVISTAWGETDTTSLALSGDTVRVS
                     ISSADKRTIVNDVLAELSVTLRMDAAVCTVPVTWVPELTDVNEEPATLRDGSLEIRAL
                     LYGDVTGDGSLTGLDAAEMLKYRVRLRPTINLTLADVTGNGDVSHLDAALVLYKILHP
                     EYVFPVLGGFVPRPSRGEPRELFWQRDGRSWALMVDDGAGIRGGELAIRSSGLRTVTA
                     PEGLVFANLTDGLLAIAFARGNVSGTLLLRIETADATAPEIVTASLDEGAIPVVWRGA
                     GAALLLAPNSPNPFNPLTTITFSLPESGNTRLAIYSADGRLVRELMNGASEAGVHRVT
                     WDGRDTEGRDAASGIYLCRLTCRSETLVRRMVLVR"
BASE COUNT          744 a         1088 c         1141 g          581 t
ORIGIN      
        1 cgaaacgacc ctcgacgtgg tggaaggcat gcagttcgac cgcgggtaca tcagcccgca
       61 tttcgtgacc gatcctgacg ccatggaggc ggtccttgag gatgccctgg tcctgatcca
      121 cgataagaag atctcctcca tcaacgacct gctccccttg ctggagaaag tcgggcaggc
      181 gggcaaagag cttctcgtga tcgccgagga cgtggaaggc gaagcgctgg cgacgctggt
      241 tgtcaacaag ctgcgtggcg tattgaaaat cgcggccgtg aaggcgccgg gattcggcga
      301 ccgccgcaag gcgatgcttg aggacatcgc ggtgctgacc ggtggcaaag tgatctccga
      361 agaagccggg ctcaagctgg agaacacgcg gatcgaggac cttggccgcg cgaaacgcat
      421 tgttgtcgac aaagacaaca ccaccatcgt tgaaggcgcc ggcgaccatg cggccattca
      481 aggtcgtgtc aaacagatcc gcaagcagat tgaagacacg acgagtgact acgaccgcga
      541 gaagctgcag gagcgccttg cgaagctggc gggcggcgtg gccgtcgtgc atgtcggcgc
      601 ggcaaccgaa actgcactga aggaaaagaa agcccgcgtg gaagatgcgc tccacgctac
      661 acgcgccgcg gtggaagagg gtatcgttcc gggcggaggc gttgcgcttc tgcgggcgat
      721 gaaagcgctc gattcgctga agcttgaagg cgatctgaaa gtcggggtag atatcgtgcg
      781 ccgcgcaatc gaagagccct gccgctggat cgttcagaat gcgggccgcg aaggtaccgt
      841 cgtggtccag aacatccttg cgaacaagga caagaactac gggttcaatg cgcagacgga
      901 agaatacggc gacctcgtca agcagggcgt tgtcgacccg acgaaagtcg tccgcaccgc
      961 gctggagaat gcggcgagtg tttcggcgct gcttctcacc accgaggcga tggttgcgga
     1021 aaaaccggag ccgaagaaac cggcggctcc tgctgcgggc ggacatcatc atccccacgg
     1081 cgggttctga gcaatcaggc cacagcgtac acggggcggc gaggcttatt cgccgccccg
     1141 ttcttttttc ccgccgaggg ttctaacgga tcacggtcac acggatcacc gaggtctgat
     1201 gtgacgcttc caaccggcac aggtacacgc cgcttgcgac ccgacgcccg gcctcgtctc
     1261 gcccgtccca gacgaaggta tgcattcctg ctgcagcggt cggcgtcagc gtgcgcactg
     1321 cgcggccgtc tgtcgcgtac accgtgagac gcgccaccac ggtgcacgga actgaggtgc
     1381 gaatggctgt tgccgagttg aacgggtttg gtgccgcgga caacgagaaa tggggcgtgg
     1441 tgcttttgtc agacgccgct accgaaaggc cgtgctcgtt gaacgtgctg aaggggtacg
     1501 gcaggagagt gagagccgtg gcgctcccgg agggaacatc gcgcttcccc cgaaaagcga
     1561 gttccaccag aggcacatcg gatagatcgc ggggatgagg agacgaaacc gacacgaaca
     1621 ccgagtcgcc aaccgcgttc cagagacaga gcgaagaggg ttcggactcg aacgcgtgag
     1681 cgcggatgaa gtccctgtcc ggccgggcca ccgtcgtgtc gatcaggaag gcgaggtccg
     1741 caccgaaaga atgtgatgac gtaatcagca cgggtaccac ggtcacggaa tcgccagaga
     1801 cggagaacgg gacgacaagc cggggcgggt caacgagagc tgccggcgcg gttcctgtca
     1861 ggatgaatgt cgccagcaca acggcgcgca gacgagatcc cccggcacga acggcctgcg
     1921 tttcagcgaa caaggaccat tctccgaacg agcgtttcgc tacggcaggt aagacggcac
     1981 aggtagatcc cgcttgccgc gtcacgtccc tccgtatcgc gcccatccca cgtaacgcgg
     2041 tgcacacccg cttcggaagc cccgttcatc agctcgcgga cgaggcgccc gtctgcggag
     2101 taaatcgcga gacgggtgtt cccggactcc ggaagcgaaa acgtgatagt ggttaacggg
     2161 ttgaacggat tcggagaatt cggcgcgagt aacagggccg cccctgcgcc ccgccacaca
     2221 accgggatcg cgccttcgtc aagcgacgcg gtgacgatct ccggcgcggt tgcatccgcg
     2281 gtttcgatac gcaggagcag ggttcccgag acgtttccgc gggcgaacgc aattgcgagc
     2341 aacccgtcgg tcaggttagc gaacacgagc ccctctggtg cggtgacggt tcgcaagccg
     2401 ctggaacgaa ttgcgagttc gccgccgcga attcccgcac cgtcgtcaac catgagtgcc
     2461 cacgaacggc cgtcccgctg ccagaaaagc tcccgtggct cgccgcgcga tggcctggga
     2521 acgaacccgc ccagcaccgg aaagacatac tcaggatgga ggatcttgta cagcacaagc
     2581 gccgcatcga ggtgcgacac atcgccgttc ccggtgacgt ccgccagcgt gagattgatc
     2641 gtcggacgca accgcacacg gtacttcagc atctccgcgg cgtcgaggcc ggtgagactg
     2701 ccgtcaccgg tgacatctcc atacaggaga gcacgaatct ccagcgaacc gtcccgcagc
     2761 gttgccggtt cctcgttcac gtctgtcagc tccggcaccc acgtaaccgg caccgtgcag
     2821 acggcggcat ccatccggag agtgacgctg agttccgcaa ggacgtcgtt cacaattgtg
     2881 cgcttatcgg cagacgagat ggacacgcgc acggtgtcac cggataacgc cagcgacgtc
     2941 gtatcggttt ctccccacgc ggtgctgatc acgcggatgg actccacaag gcctgcatcc
     3001 agcaggaaag ccatatcggc gccaaaggca tccgcgacac gtgccgtgat ggggaaaggc
     3061 acggttgttc cggcgtgcgc gataacctgt ggcaaaatga gttccgccga cggattcacg
     3121 tacgcatagg cacccatatc cgcgcgagtt ccgtcggggt cggctgggag ggcgggatcg
     3181 gccgtgttga tgcacggcga ccctgaaccg aggcggtaat cacccgcagc ggcgttgacg
     3241 aaaagcggat cggcggtagt attgccttca cccgggtacc ccgcttcgat tgcagtgtac
     3301 gtgacgtgac acgtgtccgg cgccggaatg ccgcccgaat tccacacgat gctgctgttc
     3361 agcgtgatct ccgcaccatt accgcaaccg atggccccgg tacggacttc ccccgcgccg
     3421 ttgtccacca gagtacattg cgtgacgttc accgcggact ggccaaagac gtacaaccct
     3481 ccgccttcgc ctccagcgct gttccctgca atcagcgtgt gcttcagcag caccgtgctg
     3541 ccgtagatgt aggc
//
LOCUS       DAOTNG010000313         3535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_20951_length_3535_cov_4.104598, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000313 DAOTNG010000000
VERSION     DAOTNG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20951_length_3535_cov_4.104598"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2548)
                     /gene="priA"
                     /locus_tag="P5179_12355"
     CDS             complement(<1..2548)
                     /gene="priA"
                     /locus_tag="P5179_12355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRS96052.1"
                     /translation="MPLSVDVALPLAVDAVFTYSVPEELSDGALVGMRAVVPFRRDLV
                     TGMIVAVHENGAEPAQRVLRPVADFPDPSPSLDPTMLSLTRWIADYYLSSWGEAIRAA
                     IPHGLGTNSVWMVHLSCENPHVVADNLQARFPKRAEILRCVAEKGQVSLKTLARVFSG
                     TGFRSNLQALQNDGHVKVRDEAEPIVLSRVRTEKLVQLAPAYLDPDATRRMRFGAEQL
                     RVIDGLRTISEPLSVASVRSRFGVSTATVNRLVARGVLNVEEHEVDREPDYGECEDET
                     EQLLTNDQRDALAFILPNVASRRFSAVLLHGVTGSGKTRVYLDAIEHTLDAGRGAIVL
                     VPEISLTPQTVRRFRSRFQENVAVLHSQLTAGERLDAWRALHEGRKKVAVGPRSAVFA
                     PVRDVGLIVVDEEHDGSYKQEDSDPRYHARDVAVMRARMSDSVVLLGSATPSLESLQN
                     VRRGKYTYVRLPGRVDDKPLPEVQIVDMRKERTDGNWGGLSRSLRSAVARTIENRNQA
                     VILQNRRGFSTVLQCSRCGSIVECQNCQVSMTYHRPEDAMKCHYCGDVRSVPKMCPEC
                     NETSLRFSGTGTQKVEDEISKAFPNAILLRMDQDTTHGRNAHHRLLERFRKNEASILL
                     GTQMVAKGLDFPNVTLVGVISADIGLGLPDFRAAERTFQLLTQVAGRTGRGNEPGQVI
                     VQTAMPEDPAIQAASRHDVDGFAEDELRRREELGYPPFGKLVLIQLRGSENDVVRQAA
                     RTLAVRANDLAEGKVEVLGPSEAPIPRINKQWRWHILLKGTSSQSLRAIAKGVQESFR
                     RMHSRFVQLSINVDPISLIYTRFTAPVGARLVPGPTRQPSPSTVCPVREEP"
     gene            complement(2559..3419)
                     /locus_tag="P5179_12360"
     CDS             complement(2559..3419)
                     /locus_tag="P5179_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRS96053.1"
                     /translation="MSDDTVETSDTSEARISLADRFRRAREERGLSLQDVADRSRISV
                     TILQHYESGDFSVVEPAFVRGHLRTYARIVGVEEREIDSVFPPSGLSVSGPTALEDDE
                     AQEALFIEKRPALPAIPWKTAAWFGLGAALLVALWLWHPWHSPENSFAESPPEVESAV
                     APAQKENAEITAPAENDTLSDEPAVRLATPPELAKARVPGVVRTMTIEATDSVWVLIA
                     RKDGMVLFDGILVPGQSKQSDVEDTLRVTLGRYWAARLLLNRQAVPTPRQEARNMTFF
                     LCTPSGIVPP"
BASE COUNT          729 a         1071 c          984 g          751 t
ORIGIN      
        1 aaggttcttc tcgaaccggg cacaccgtgg atggtgaagg ttgccgtgtc ggtcctggaa
       61 ccagtctggc acccaccggc gcagtgaagc gtgtatagat cagagatatg ggatccacat
      121 ttatggaaag ttgtacaaaa cgtgaatgca tacgcctgaa tgattcctgg actcccttcg
      181 cgatagcgcg gagactctga ctcgatgtcc ctttgaggag tatgtgccag cgccattgct
      241 tgttgattct tggaatgggc gcctctgacg gtccaaggac ttcgaccttt ccttctgcga
      301 ggtcgttcgc tctcaccgcg agtgtgcgag cggcttgacg gacgacatcg ttttccgaac
      361 cacggagctg tatcagcaca agcttcccga agggcgggta ccccagctct tcccgccttc
      421 gcaactcgtc ttccgcaaaa ccatcaacat catgccgact cgccgcctga atcgccggat
      481 cctccggcat cgccgtctgg actatcacct gtccgggttc gtttccgcga cccgtcctgc
      541 ctgcaacctg agtcaggagt tgaaacgttc tttcggcggc ccgaaaatca ggcagtccca
      601 gtccaatgtc tgcagagatc acccccacaa gggtaacgtt cgggaagtcg agccccttcg
      661 cgaccatttg ggttccgagg aggattgaag cctcgttttt tcggaatctc tccagtaatc
      721 tgtggtgcgc gtttctcccg tgggttgtgt cctgatccat gcgaagcaga atcgcgttcg
      781 ggaaggcttt ggagatttcg tcctccactt tctgcgtacc tgttcccgag aatcgtaacg
      841 aagtttcgtt gcattccgga cacatcttcg ggacggaccg aacatcgccg caatagtgac
      901 atttcatggc gtcttccgga cggtgatatg tcatcgacac ctggcagttc tggcattcta
      961 cgattgaacc gcacctggaa cattgcagca ccgttgagaa gccgcgcctg ttctgaagga
     1021 tgactgcctg gttcctgttc tcgatagttc tggcaacggc ggaacggagc gaccgggaga
     1081 gaccgcccca gttaccgtct gtgcgctctt tccgcatgtc cacaatctga acttccggca
     1141 gcggtttgtc atcaaccctc cccggaaggc gaacgtaggt gtacttccct cgacgcacgt
     1201 tctggagcga ttccagggag ggcgtcgcgg aaccaagcaa cacgacgctg tcgctcattc
     1261 gtgcgcgcat gacggcgacg tccctcgcgt ggtacctcgg atcagaatcc tcctgtttgt
     1321 aactcccgtc gtgctcctcg tccacaacta tcaacccgac atcacgtacg ggcgcgaata
     1381 ctgccgacct ggggcctact gccaccttct ttcttccttc gtggagcgcc cgccacgcat
     1441 caaggcgttc gccggcagtc aactgactgt gcaagaccgc gacgttttcc tggaaccgtg
     1501 accgaaatcg ccgcaccgtt tgcggcgtca gactgatttc ggggaccaga acaatggcac
     1561 cgcggcccgc gtcaagcgtg tgttcaattg cgtcgaggta cacccgcgtt ttgccactgc
     1621 cggtaacgcc gtgcagcaga accgcggaaa atctccggga ggctacgttc ggcagaatga
     1681 atgcgagcgc gtccctctgg tcgttggtca gaagttgttc agtttcgtct tcgcattcgc
     1741 catagtcagg ttcccggtca acctcgtgct cctctacgtt gaggactccc cgcgcaacaa
     1801 gacggttcac cgtggcggta gaaactccga accgagatcg gacactcgcg acggaaagcg
     1861 gctcagaaat cgtccgcaaa ccgtcgataa cccggagttg ttcagcccca aaacgcatac
     1921 ggcgagttgc atccggatcc agatacgcgg gagccagttg gaccagcttt tccgtccgga
     1981 cccgggaaag aacgattggc tccgcttcgt ctctgacttt gacatgtccg tcattctgca
     2041 aagcctgaag attcgagcga aagccggtcc ccgaaaacac gcgggcgaga gttttcagtg
     2101 acacctgacc tttttcagcg acacaccgca ggatttccgc cctttttggg aagcgcgcct
     2161 ggagattatc cgcgaccacg tgagggttct cgcacgaaag gtgaaccatc catacactgt
     2221 ttgttccgag accgtgagga atcgccgctc gtatggcctc cccccagctc gacagatagt
     2281 aatcggctat ccagcgagtg agagaaagca tggtggggtc gagggacgga gaagggtcgg
     2341 ggaaatcagc tacgggtctc aggacacgct gggcgggctc agcaccgttc tcgtggacgg
     2401 caacaatcat tccggtaacg aggtctcgcc ggaacgggac aacggcacgc atgcccacga
     2461 gtgcgccgtc tgacagctcc tcaggaacgg aataggtgaa tacggcgtcg accgccaacg
     2521 gcagcgcaac gtcaaccgag agaggcatca cctgtttctc acggaggcac gattcccgat
     2581 ggagtacaca aaaagaaggt catgttgcga gcctcctgcc gcggagtagg aacggcctgc
     2641 cgattcaaca gaagcctcgc cgcccagtag cgtccaaggg taacccgcaa cgtgtcttcc
     2701 acatcactct gtttgctctg gccgggcacg agaatcccgt cgaaaagcac catgccgtct
     2761 tttcgtgcga tgagcaccca aacgctgtcg gtggcctcta tggtcatcgt tctcaccacg
     2821 ccgggaaccc tcgctttcgc caactccggt ggcgttgcaa gtcgaactgc gggttcatct
     2881 gagagcgtgt cgttttctgc cggcgcggtg atctcggcgt tttccttctg ggctggagca
     2941 accgcgctct ctacttcggg aggggattcc gcgaatgaat tttccggact atgccagggg
     3001 tgccagagcc agagtgcgac aagcagggct gcacccaatc caaaccaggc cgctgtcttc
     3061 catggtattg cgggaagcgc aggtcgtttc tcaatgaaga gggcttcctg cgcttcatcg
     3121 tcttccaggg ccgtcgggcc ggaaacggag agcccggacg gcggaaagac tgagtctatt
     3181 tctcgctctt caacaccgac aatccgggcg taggtccgca ggtgcccccg aacgaacgcc
     3241 ggctcgacga cggagaaatc ccccgattca tagtgctgga gaatcgtaac actgatgcgc
     3301 gaacgatccg ccacatcctg cagactcagc ccacgttctt cgcgcgctct acgaaagcgg
     3361 tccgcaagac tgattcgcgc ttcggaggtg tctgaggttt caacggtgtc gtcagacatc
     3421 cctattcctt ccgtcgagcg acaatgagct gcagcatggc cactacacgg cttacgggca
     3481 aacccacgac gttggaatag cacccgcata tcttcgtcac aaaccccgct gccat
//
LOCUS       DAOTNG010000314         3528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21006_length_3528_cov_4.360207, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000314 DAOTNG010000000
VERSION     DAOTNG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21006_length_3528_cov_4.360207"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="P5179_12365"
     CDS             complement(<1..1733)
                     /locus_tag="P5179_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96054.1"
                     /translation="MANGQERAPGRTPERNWTIYLVQHSHTDIGYTASQHEMLPAHLQ
                     YLDMALDCCDRTDGYPDEARFRWTCEAAWPVREYIRRRPARQIDRLRARCAEGRIEPT
                     ALMFNMSEITPEAGLVASLQPVREINDALGIRIRTAMQNDVNGVAWAMADYLPPCGVR
                     YLTMGVHRYRSVLPFKVPTAFWWESPSGNRMLAYRADHYLTGNWLGFNPESSLEHWMS
                     MGAADRPVLETSLAEYLASLEERGYPYSHLMVQYSGHETDNSHPSTDQCELIRRWNET
                     HERIVLKSATAGEFLRWLEEHHGQGLPALRVAWPDWWTDGFGSAARETGASRDAHAAL
                     SATNALLAMARASGADIPEASITRLTEAREQVLFYDEHTFGSVDSVADPESPYTCEQW
                     SEKASYVWQGVQNSALAREEALGYLASVNDAATQPVVRVYNTLNWTRSEPAQVFVPFD
                     VVARGYAVKITDPGTGAACPAQVVYDRPDDRPKETILAYDRPAGRFVTFMAPEVPALG
                     WKDFCLETGPEEDDDAPADPLDVRRIENRWFSLSLDNRTASLRSIRHRPTGRELVDTE
                     SQWRLGQVIYE"
     gene            complement(1837..2664)
                     /locus_tag="P5179_12370"
     CDS             complement(1837..2664)
                     /locus_tag="P5179_12370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS96055.1"
                     /translation="MAEWAPTALSAVIPGISFRFLQPHFRLPFRAASTRMLRLLRIPV
                     EHVNTRIEDVDPFTKQQIRKLCAMPRMSTFAMGVLINRLVAQMAEDEAYVNVGVWHGF
                     TYFAGVLSNGGKQCIAVDNYSSADRFSGQKGIKRLLDYYPRKAKIEAARAAFRRRMAR
                     WGGEHHRLFEMDYREYFQYVHEAPIGVYLYDGDHSYENQLQGLKLAEPFFSKNCVVIV
                     DDCNDEAVYRATRDFIDQSHNSYAILLFQRTCCDRHPTLWNGTMVFQRVPRPHPVPF"
     gene            2931..>3528
                     /locus_tag="P5179_12375"
     CDS             2931..>3528
                     /locus_tag="P5179_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96056.1"
                     /translation="MKQPLKTTISPEHPLLVLMWMPCADPVDEARQLVALWEGLDDVI
                     RPHVSVQIGRHHPERNRVLLAHAQAAGVPITLQIQGDNGDRTDTVPMEEIRALVDSFP
                     CIVGLEIGEASQRTFSDHGSGPEYSMGRNARYARDAIRIAGEHGLYFTWQLQRDSFLA
                     IACSADNEALFDAVCEHRAHVIPMHEMNGEFEKHANHTG"
BASE COUNT          683 a         1089 c         1080 g          676 t
ORIGIN      
        1 ctctcgtaga tcacctgccc cagccgccac tgcgattcag tatccaccag ttcgcggccg
       61 gtgggccggt gccgaatact cctgaggctg gcggtgcgat tgtcgaggga gagcgagaac
      121 cagcggtttt cgatcctgcg gacgtccaac ggatccgccg gggcgtcgtc gtcctcttcc
      181 ggaccggttt cgaggcagaa atccttccac ccgagtgcgg gaacctccgg ggccatgaaa
      241 gtgacgaaac ggcccgcggg gcggtcgtat gcgagaatcg tctccttcgg acggtcatcg
      301 ggccggtcgt acaccacctg cgccgggcat gccgcgccgg tgccggggtc ggtgatcttg
      361 acggcatacc ctcgcgccac cacatcaaac ggaacaaata cttgagccgg ttcgctgcgc
      421 gtccagttga gcgtgttgta cacccgcacc accggctgcg tggctgcgtc attcaccgag
      481 gcgaggtaac ccagcgcctc ctcgcgcgca agcgcagagt tctgcacccc ctgccacacg
      541 tacgacgcct tttccgacca ttgctcgcac gtgtaaggcg attccggatc ggcaacgctg
      601 tccaccgatc cgaaggtgtg ttcgtcgtag aacaggactt gttcgcgggc ttcggtcagc
      661 ctcgtgatgg acgcttccgg aatatccgcg ccggaggcgc gcgccatggc cagcagggca
      721 tttgtggcgg acaacgcggc gtgcgcatcc cgcgaagcac cggtttcccg cgctgccgaa
      781 ccgaacccgt ccgtccacca gtccggccat gccactctga gcgcggggag gccctgaccg
      841 tggtgttctt ccaaccaccg gaggaactcc cctgcggtgg cgcttttcag aacgatacgt
      901 tcgtgcgttt cgttccagcg tcgaatcagt tcgcactggt cggtagaggg gtgcgagtta
      961 tccgtctcat ggcccgagta ctggaccatg agatgtgagt aggggtaccc gcgctcctcc
     1021 agcgaggcga ggtactccgc aagcgatgtt tcaagcacgg gacggtctgc cgcgcccata
     1081 ctcatccagt gctccaggct gctttcgggg ttgaacccga gccagttccc ggtcaggtag
     1141 tgatcggcgc ggtaggcgag catccggttg cccgacggag actcccacca gaacgctgtt
     1201 ggcaccttga atggcaggac ggagcgatac cggtgcacgc ccatcgtcag gtagcgaacg
     1261 ccgcacggag gcagataatc tgccatggcc caggcgacgc cgttcacgtc gttctgcatc
     1321 gcagtccgga tacgtatccc cagcgcgtcg ttgatttcgc ggacgggttg gagcgacgcc
     1381 acgagacccg cttcgggagt gatttccgac atgttgaaca tgagcgcggt cggctcgatt
     1441 cggccttcgg cgcatcgggc tctcaggcgg tctatctggc gcgccggtct gcggcggatg
     1501 tattcacgaa ccggccacgc ggcctcgcac gtccaccgga agcgggcttc atccgggtac
     1561 ccatccgtac ggtcgcagca gtcgagcgcc atgtcgaggt actggaggtg ggcgggcagc
     1621 atttcgtgct gagatgccgt gtacccgata tcggtgtggc tgtgctggac aagatagatg
     1681 gtccagttac gctccggcgt gcgcccgggg gcgcgttctt gtccgtttgc catggcgtgg
     1741 tggcctcaac agggggacgc tctgtcgcac gaaagggaat cccgtcgtgt cagtggaaag
     1801 ggtatggaga ggcggggcgc gaggcaacgc tgcgtgtcag aagggtaccg ggtggggacg
     1861 aggcacacgc tggaacacca ttgtgccgtt ccacaacgtg ggatggcgat cgcaacaggt
     1921 gcgctgaaac agaagaatgg cgtaggagtt gtggctctga tcaatgaaat cccgtgttgc
     1981 ccggtacacg gcttcgtcgt tgcagtcgtc cacaatcacc acgcagtttt tggagaagaa
     2041 cggttcggcg agtttcagac cctgcaactg gttctcgtac gagtgatcgc cgtcgtagag
     2101 gtacacaccg atgggagctt cgtgcacgta ctggaaatac tcgcggtaat ccatttcgaa
     2161 cagacggtgg tgttcgcctc cccacctcgc cattctccgg cggaatgccg ccctcgcagc
     2221 ttcgattttt gcctttctcg ggtagtaatc cagcaaacgt ttgatgccct tctgtccgga
     2281 gaatctgtct gcggaggaat agttgtccac tgcgatacac tgcttgccgc cgttgctcag
     2341 gacacccgcg aaataggtga aaccatgcca tacacccacg ttgacgtatg cctcgtcttc
     2401 ggccatctgt gcaacgagtc ggttgatcag aacccccatg gcgaacgtgg acattctcgg
     2461 catcgcacac agcttccgaa tctgttgttt cgtaaaggga tccacgtctt ctattctcgt
     2521 gttgacatgc tccaccggaa ttcgcaggag acgaagcatc cgtgtggaag cggcgcgaaa
     2581 cggcagacgg aaatgcggct ggagaaaacg gaatgagatt ccgggaatga ccgcgcttaa
     2641 cgcggtcggg gcccattcag ccactgcgcg attcctccgg tagtgcgcca ggatacagag
     2701 cttccgtacc ctcagcggtg ccccccacga acagccgtac gagacaaaac gcgtcgccgc
     2761 cacgcgacac acctgtccag cccggcccga ctcctcaaca gacaaagtaa acgctacgaa
     2821 tgtgaaagat gcatggagat tccgtgaatg tttatttaga ttcccttacg aactattgca
     2881 agcccctcca gccggggacg cctgatttac cgcccacgag gtatcccgcc atgaaacaac
     2941 cgttgaaaac cacgatttcc cctgagcacc cccttctcgt cttgatgtgg atgccgtgcg
     3001 ccgatccggt ggatgaagcc cgccagcttg ttgcgctctg ggaggggctt gacgacgtaa
     3061 tccgccccca cgtgtcggtt cagattggac ggcaccaccc ggaaagaaac cgggtgttgc
     3121 tcgcgcacgc gcaggccgcc ggtgtgccga tcaccctcca gattcagggc gacaacggcg
     3181 accggacgga tacggtgccc atggaagaaa tccgcgcgct ggtggactcg ttcccctgca
     3241 tcgtcggact ggagatcggg gaagcgagcc agcggacgtt cagcgaccac ggcagcggcc
     3301 cggaatactc catgggccgg aacgcccggt atgcccgcga cgcgattcgg atcgccggcg
     3361 aacacgggct ctacttcacc tggcagctcc aacgggatag cttccttgca atcgcgtgct
     3421 ccgccgacaa cgaagctctg tttgacgccg tctgcgaaca ccgggcacac gtcatcccga
     3481 tgcacgaaat gaacggcgaa ttcgaaaaac acgccaacca cacagggg
//
LOCUS       DAOTNG010000315         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21024_length_3525_cov_5.724207, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000315 DAOTNG010000000
VERSION     DAOTNG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21024_length_3525_cov_5.724207"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="P5179_12380"
     CDS             complement(<1..1164)
                     /locus_tag="P5179_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS96057.1"
                     /translation="MALVELVREAGVVGAGGGGFPTHVKLAGKADTVIANGAECEPLL
                     HKDAAVMEREAARVVAGMLATMEAVGATRGIVGIKKKNSRAIEMITAASAGQPISVHL
                     LGDYYPAGDEYDLVYETTGRLIPPAGIPLNVGAVVCNVETFANISRAAENLPVTEKTV
                     TIGGAVRSPVTLTVPVGAPFRDVIEAAGGLTTDDPVLCLGGLMMGTTSDNLDTPVLKT
                     TTGAIVLPRTHHVVRRKLKPWNHQAKIGKSACDQCRFCTELCPRYLLGYPVEPHRVMR
                     SLGFTATDRRLLDEWAALCCSCGLCTLYSCPEELFPKEACDTSKDELRKAGFKWTTQS
                     QVRPHPLREGRRVPIRGLMRKLVVEEFDHPAHWEDLGVDPGRVTLPLKQAAGAP"
     gene            1279..2124
                     /locus_tag="P5179_12385"
     CDS             1279..2124
                     /locus_tag="P5179_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS96058.1"
                     /translation="MIDVHMHIGKLYVGEKPLTPRHLLRFMDANDIEKAILLPIESPE
                     EAHYYVSTEYVMRVARKYPDRFVAGCNVDPRIGTGDNNGILRARLTDYRDRGCKAYGE
                     AMSGLAIDDPALQRVYRICGELRMPVIYHIDGFRNVDEKGFPRFERMLRMFPETVFVG
                     HAQHFWAEISGDVTEAEFSSYPKGPITPGGAVPRLMETYPNLFADLSAGSAYNALTRD
                     PPFGYAFLERFQDRLFFGTDVCRFVQPIDVIPFLKEAREQERISEIAFEKITRLNAMR
                     VFGLK"
     gene            complement(2135..2842)
                     /locus_tag="P5179_12390"
     CDS             complement(2135..2842)
                     /locus_tag="P5179_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS96059.1"
                     /translation="MVVVGGSGRVGTGVLPYLASWHEVVNFDIRPPSVPAGRYVRGSL
                     FDTELLGRVLTQADGVIFMALGVWPPEVDASYEINVRGVHYVLEAARQAGLMHVVHSS
                     TASVHDESKGSFPDEEMPLEPLHVYGLTKGLGETVCRWFCRMYGMSVLALRLFAPQPY
                     ENWLAQCRTRMPNGWTTFLDTARAYDAALRATSHSGFDAVFISGDWTGTYVNCAKAKR
                     VLGWEPLDRYEGEKQNS"
     gene            complement(2870..>3525)
                     /locus_tag="P5179_12395"
     CDS             complement(2870..>3525)
                     /locus_tag="P5179_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRS96060.1"
                     /translation="EMAKEAQRAKGLGFRVHKIKAHRPADTYELVKLITEACGPDFLL
                     RLDPNTHFHDLPTAIRIARSLEGFNIEVYEDPFRFEDMSDYRQFRAKIDVPVARHLGS
                     PRDVFLWLKNEAMDTFNCGGNVEAMRTFDAIARGAGVPMWGQMFAFGSCIASMFAVHI
                     AATLPACTMALDELPHIRVDDLSGGSFVIEKGAVKVPDTPGLGVQLDMQAVEKYRIR"
BASE COUNT          712 a         1091 c          989 g          733 t
ORIGIN      
        1 cggtgcaccg gccgcctgtt tcaacgggag ggttacccgg ccgggatcta cgcccagatc
       61 ctcccagtga gccgggtgat caaactcctc tacaaccagt ttcctcatga gcccccggat
      121 cggaacgcgc cgcccttccc gaagggggtg agggcgcacc tgagattgcg tggtccattt
      181 gaaaccggct ttccgcagtt cgtccttgga cgtatcacac gcctctttcg ggaaaagctc
      241 ttcgggacag gaatacagcg tgcaaagtcc gcaactgcaa cacagggcgg cccactcgtc
      301 gagaagtctt ctgtcagttg cggtaaaccc gagactgcgc atcacgcggt gaggctccac
      361 ggggtagccc agcaggtacc gcgggcacaa ttccgtgcag aagcggcact gatcgcaggc
      421 tgacttcccg attttcgcct gatggttcca aggtttgagt ttgcgccgga cgacgtgatg
      481 ggtgcgcggg aggacgatgg cgccggtcgt tgttttcagc acaggagtgt cgagattgtc
      541 cgatgtcgtt cccatcatga gcccgcccag gcacaggaca ggatcatccg tggtcagccc
      601 gcccgccgct tctatcacgt cacggaacgg cgctcccaca ggcaccgtaa gagttacggg
      661 agagcgcacc gcgcccccga tggtgacggt tttttccgtg accggcaggt tctctgctgc
      721 tctcgagatg ttcgcgaatg tttccacgtt gcacaccacc gcacccacgt taagcgggat
      781 gccggcaggc ggaatgaggc gtcccgttgt ttcgtacacg agatcgtact catcgcccgc
      841 cggataataa tcgccgagaa ggtggactga aatcggttgc ccggcgctcg cggcggtgat
      901 catttctatt gcccgtgagt ttttcttctt gatgcctacg ataccacgcg ttgcccctac
      961 ggcctccata gtggccagca ttccggcgac gacgcgggcg gcctctcgtt ccattactgc
     1021 tgcgtctttg tggaggagcg gctcgcactc agctccgttg gcgatgacgg tatcagcttt
     1081 ccctgccagc ttcacgtggg tcggaaatcc gccgccgccc gcgcccacta cgcccgcttc
     1141 gcggacgagt tccaccaatg ccatgttttc atccctgtgg tttgcgatct caagttgcta
     1201 atttgaaaga gcgaaaagtg gcagacaatg ccaaggggta gccccaaaca cgcgatcggt
     1261 ggagacggag caggagccat gatagacgtt catatgcata tcgggaaact ctatgtcgga
     1321 gaaaaacccc tgaccccccg gcacctgctc cgattcatgg acgcgaacga catcgagaaa
     1381 gcgatcctcc tgccgattga aagcccggaa gaagcgcact actacgtatc cacagaatat
     1441 gttatgcgtg tcgcccggaa atacccagac cggtttgtcg ccggctgcaa cgtagacccg
     1501 cgcatcggaa ccggtgacaa caacggcatt ctccgggcgc gcctcacgga ttaccgggat
     1561 agaggatgca aggcgtacgg cgaggccatg agcgggcttg cgatagacga cccggctctg
     1621 cagcgcgtgt acagaatctg tggcgaattg cgcatgccgg taatctacca cattgacggg
     1681 ttccgcaacg tggatgaaaa gggctttcct cgttttgagc gcatgttgag gatgttcccg
     1741 gaaacggttt tcgtcgggca cgcgcagcac ttctgggcag agatctcagg tgacgtaaca
     1801 gaagccgagt tcagcagtta tccgaaggga cccatcacac cgggcggagc agttccgcgc
     1861 ctgatggaaa cgtaccccaa cctcttcgcc gacctttccg cgggaagcgc gtacaacgca
     1921 cttacccgcg atcctccgtt cggctacgcc ttcctcgaga ggtttcagga tcgcttgttc
     1981 tttggaacgg atgtgtgccg gttcgtccag ccgatcgacg tgattccgtt tttgaaagaa
     2041 gcccgtgaac aggaacgtat ttccgagatc gcattcgaga agatcaccag gctcaacgcc
     2101 atgcgcgttt tcgggctgaa ataacgcggg cagactagct attttgcttc tccccttcgt
     2161 acctgtcgag cggctcccaa ccgagcacgc gcttcgcttt cgcgcagttc acgtacgtgc
     2221 cggtccagtc tccggaaatg aacacggcgt cgaacccgct gtgtgacgtt gcgcgcaacg
     2281 ccgcatcgta cgcacgcgca gtatcaagaa acgtcgtcca tccgttcggc attctcgttc
     2341 ggcactgtgc cagccagttt tcgtagggct ggggtgcaaa aagcctcaag gccagcacgg
     2401 acatgccgta catgcggcaa aaccatctac acactgtttc cccgagaccc ttcgtgagtc
     2461 cgtacacgtg caggggttcc agaggcatct cctcgtccgg gaaactcccc ttggattcgt
     2521 cgtggacgga ggcagtactg gaatgcacca cgtgcatcag ccctgcctgc ctggcggcct
     2581 ccaacacgta atgcacaccc cggacgttga tttcgtaact ggcgtcgacc tcaggtggcc
     2641 atacgcccag cgccatgaat atcaccccgt ccgcctgcgt cagcacgcgg ccgagcagtt
     2701 ccgtgtcgaa cagtgatccc ctgacatacc gccctgccgg aaccgacggg ggtctgatat
     2761 cgaaattgac cacctcatgc cagctcgcga ggtagggcag aaccccggtt ccgaccctgc
     2821 ctgacccgcc cacaactacc accctcaacg cgcgaccctt tcgtttttct caacggattc
     2881 ggtatttctc aaccgcctgc atgtcaagtt gaacccccaa tccgggcgta tcagggactt
     2941 tcacggcacc tttctcgatc acgaaacttc cgccggagag gtcatccacg cgtatgtgtg
     3001 gcagttcgtc cagcgccatc gtgcatgccg gcagcgtcgc cgcgatgtga accgcaaaca
     3061 tgctggcgat gcaggagcca aacgcgaaca tctgtcccca catgggtacg cccgcgcccc
     3121 gggcgatcgc gtcaaatgtt ctcatggctt cgacgtttcc cccgcaattg aacgtatcca
     3181 tggcttcatt tttgagccag agaaacacat cccgcggaga gcccagatgg cgtgcgaccg
     3241 ggacgtcgat tttcgcccgg aactggcggt agtcggacat atcctcgaac cgaaacggat
     3301 cttcgtatac ctcgatattg aacccctcaa gagaccgcgc gatccggatc gctgtgggca
     3361 gatcgtggaa atgcgtgttt ggatcgagcc gtaggaggaa atctggtcca cacgcttccg
     3421 taatcagttt caccagctca tacgtgtcgg ccggacgatg ggctttgatc ttgtggaccc
     3481 ggaagccgag tccttttgcc ctctgtgcct ctttggccat ttcga
//
LOCUS       DAOTNG010000316         3505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21181_length_3505_cov_4.983478, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000316 DAOTNG010000000
VERSION     DAOTNG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21181_length_3505_cov_4.983478"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="P5179_12400"
     CDS             complement(<1..882)
                     /locus_tag="P5179_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS96061.1"
                     /translation="MIRTDVSITAEALLPQIEAAWGLSASKIDLLTRNRSATDGSPVF
                     TVRGQYTSKGWTEWTQGFEFGSALLQFDATDERRFLDFGRSKTRAVMASHITHMGVHD
                     HGFNNISTYGNLRRLILEGRFASDPAELEFCELALKCSGAVQARRWTPTAEGGGFIYS
                     FNGPHSLFADTMRSLRSLSLAHRLGHVLLEEQDARVSLLSRMVAHAETTARYAVFYGE
                     GRDAYDVRGRVAHESIFNVNNGVYRCPNSQQGYSAFSTWTRGLAWVMLGFAEELEFLE
                     TVPDGELEPLGGRTRVEA"
     gene            1083..>3505
                     /locus_tag="P5179_12405"
     CDS             1083..>3505
                     /locus_tag="P5179_12405"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS96062.1"
                     /translation="MGKKRDLGAALLCKPVSLESLFNWPKQVPDDEWWSNIATRVRAF
                     PEGKQVSWGIPFRLGKGKNKRVIMLARDTQDVTVSLPADNASSISYLCLLHAWFEKLP
                     DDKTLPPQEGLPVAEYVITYDDESAHVQTVRARFEVPMASSPGPPWLAVQFDAWTAID
                     PTKPTTDMGWGVAQFGFGKEHVRQQPAIVLYALPNPHPERKPASLTIRGLVDAPFVVA
                     GLTAYFGEQHPLRYLPRRHYRVTFDDMPRHVTRAELDLGEITRIERTTGPRNEEWLGA
                     PHAGVSGAREPDTGAEDLIEAAGAPDATMSLRLEGKKKAYSFRLGEVFEKGVSESGKA
                     KLEVIGRQRQWLNVRIIDSSTGRPTPVRVHFSGPHGEYLPPYGHHSQVNTNWFEDYGA
                     DVRVRDMSYAYVPGEFRTELPVGDVFVEINKGFEYQPTRARVTVQPGQKELELRVNRW
                     KDLRSAGWVTADTHVHFLSPHTAWLEAQAEGVNVVNLLASQWGRLFTNVGDYIGRVGV
                     AENDTIVYVGTENRNHMLGHMSMLGTQGMPVVPMCAGGPTEAWIGDPDYRTLAEWAME
                     NRQKGGVVIRPHFPYCGCTEDPVPIIAGLVDALETGSLQGGNFPLQEWYRYLNCGYRV
                     AVAGGTDKMSASCPLGWIRTYARVDTNEPFTYDAWARAVRAGRTISTTGPLIDLSVDG
                     HGVGDVINVRTRAGTYEVDAIAESAWSLGKIEVVCNGRVVASEESAAGAKTLRCKANV
                     VLSGSGWIAARCVGHANQPGYLVAHTSPVYIAARGSVLFDGPAAEHMLQLVNGGIEYL
                     RTLSTPYDDA"
BASE COUNT          730 a         1054 c         1064 g          657 t
ORIGIN      
        1 cgcctcgaca cgcgtccggc ctcccaaagg ctccagctcg ccgtctggaa ccgtttccag
       61 gaattccagt tcctccgcaa agccaagcat cacccatgcc agccctctcg tccacgtgct
      121 gaacgctgaa tagccttgct ggctgttcgg gcagcggtac accccgttgt tgacgttgaa
      181 tatgctctca tgtgccactc gcccgcgcac gtcatatgcg tcacggccct cgccgtagaa
      241 aacagcataa cgggccgtcg tttccgcgtg ggcaaccatt ctcgataaca gcgacacccg
      301 cgcgtcctgt tcttccagaa gaacgtgccc caggcggtgc gcgagggaca aagagcgcaa
      361 actgcgcata gtatcggcaa aaagcgaatg cgggccgttg aacgaataaa tgaacccccc
      421 gccctccgca gtgggtgtcc accgccgtgc ctgcactgcg ccgctgcatt tgagggccag
      481 ttcacagaat tcgagttccg ccgggtccga cgcgaatcgt ccctccagga tcaggcgacg
      541 caggttcccg tacgtgctga tgttgttgaa accgtggtcg tgaactccca tgtgcgtgat
      601 gtggcttgcc atgaccgcgc gcgttttgct cctgccaaag tcgaggaaac gccgctcgtc
      661 ggtcgcgtca aactgaagga gcgcggaacc gaactcaaat ccctgcgtcc actcggtcca
      721 gcctttggag gtgtactgcc cacgcaccgt aaatacaggg gatccgtcgg ttgcagacct
      781 gtttctggtc agcagatcga tttttgaagc ggaaagcccc cacgcagctt cgatttgtgg
      841 gagaagggcc tccgcggtaa tgctcacgtc cgtgcgaatc atcagatagt tccagaaaac
      901 gcgtgttgcc gcgagaacca cgctatcaga atgtacagag gtgaaggata acgcgaaacc
      961 cggacgcatg gtggcaccgg gcacggaagg ggtggttgtg tccctacggc aagttcgtta
     1021 ccctaccgtc atatcatcgc gccttttgaa ccatgtggga aggccacgaa cgggggtgtt
     1081 ccatgggaaa gaaacgtgat ctcggtgcgg cgctcctctg caaacctgtc tccctcgaaa
     1141 gtctcttcaa ctggccgaag caggttcccg atgacgaatg gtggagcaat attgcgaccc
     1201 gggtcagggc attcccggaa gggaaacagg tctcctgggg catcccgttc cggttgggca
     1261 agggcaagaa caaacgcgtt atcatgctgg cgagggatac gcaggacgtg acggtctcat
     1321 tgccggcgga caatgcctct tcgatttcgt atctctgcct tcttcatgcg tggttcgaaa
     1381 agttgccgga tgacaaaacc ctgcctccac aggaaggttt gccggtcgcc gagtacgtaa
     1441 tcacgtacga tgacgagagc gcgcatgtgc aaacggtgcg tgcccggttc gaggtcccga
     1501 tggcgtcaag ccccggccca ccgtggttgg ccgtccagtt cgacgcgtgg accgcgattg
     1561 atccgacaaa accgaccacc gatatgggct ggggtgttgc gcagttcggg ttcggaaagg
     1621 aacatgttcg gcaacagccc gcgatagttc tgtacgccct ccccaacccg cacccggaaa
     1681 gaaaacccgc ctccctcacc atccgcggat tggtcgatgc tcctttcgtc gttgccggtc
     1741 ttacggcgta tttcggcgag cagcacccgc tgcgatacct gccgagacgg cattatcggg
     1801 tgacgttcga cgacatgcca cgacacgtca cacgggcaga gctcgacctg ggcgagatca
     1861 cgcgaatcga acgcaccacc ggtcccagaa acgaagaatg gctcggggcg ccccatgccg
     1921 gggtatcggg cgctcgtgaa cccgacaccg gggccgaaga cctcatcgaa gccgcgggag
     1981 cacctgacgc gacgatgtct ctgcggctcg aagggaagaa aaaagcgtac tcgttccgct
     2041 tgggcgaggt cttcgaaaaa ggcgtctcgg agtccgggaa agcgaaactc gaggtaatcg
     2101 ggcggcagcg acaatggctg aatgtccgga tcatcgactc gagcaccggc aggccgacac
     2161 ctgtccgggt tcatttttcc ggaccgcacg gagaatattt gccaccctac gggcaccatt
     2221 cccaggtgaa cacgaactgg ttcgaagact atggcgcgga tgtccgtgtt cgcgacatga
     2281 gctatgcata cgttccgggc gaattccgga ccgaattacc cgtaggcgac gtgttcgtgg
     2341 agatcaacaa ggggtttgaa tatcagccca cacgcgccag ggttacggtt caaccgggac
     2401 aaaaggaact ggaactccgc gtcaatcgct ggaaggacct cagaagcgcc ggatgggtta
     2461 ccgccgatac gcacgtccat ttcctgagcc cgcataccgc gtggctggaa gcgcaggctg
     2521 agggcgtcaa cgtggtgaac ctgctggcat ctcagtgggg gcggcttttc accaacgtcg
     2581 gagactacat cggtcgggtg ggtgtcgcgg aaaacgatac cattgtgtac gtgggcaccg
     2641 aaaaccggaa ccacatgctc ggccacatgt ccatgctggg aacgcaggga atgccggttg
     2701 ttcccatgtg cgccggggga ccaacggaag cctggatcgg tgacccggac taccggacgc
     2761 tcgccgaatg ggcgatggaa aaccgccaga aaggcggcgt agtcatcagg ccgcatttcc
     2821 cctactgcgg gtgtacggag gatcccgtac ccatcatcgc cggcctcgtc gacgcactgg
     2881 agaccggctc cctccaaggc gggaatttcc cattgcagga atggtaccgt tacctgaact
     2941 gcgggtatcg tgtcgcggtt gccggtggca cggacaagat gagcgcgtcg tgtcccctcg
     3001 gctggatccg cacctacgcg cgggttgata caaatgaacc cttcacctac gacgcatggg
     3061 cacgcgccgt ccgggccggc cgcacaatct ccactaccgg gccgctcatt gacctttcgg
     3121 tggacgggca tggcgtggga gacgtgatca atgtgaggac ccgtgcgggc acctacgagg
     3181 tggatgccat cgcggaatca gcgtggagcc tcgggaaaat cgaagtggtg tgcaacggcc
     3241 gtgtggtggc gtcggaggaa tccgccgccg gagcgaaaac gcttcgctgc aaggccaacg
     3301 ttgtcctctc gggaagcgga tggattgctg cccgctgcgt ggggcacgca aatcagccgg
     3361 gatatctggt ggcccacacg tctccagtat atatcgccgc ccgcggttcc gttctttttg
     3421 acggccccgc cgccgaacac atgctccagc ttgtcaacgg aggaattgag tacctgcgca
     3481 cgctctccac gccgtacgac gatgc
//
LOCUS       DAOTNG010000317         3497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21223_length_3497_cov_6.045032, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000317 DAOTNG010000000
VERSION     DAOTNG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21223_length_3497_cov_6.045032"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1542)
                     /gene="gcvPA"
                     /locus_tag="P5179_12410"
     CDS             complement(199..1542)
                     /gene="gcvPA"
                     /locus_tag="P5179_12410"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRS96063.1"
                     /translation="MADPHPFIPCTDSDLAEMLAAIGVRSFEDLVAPVPDAIRAASAL
                     NLPPRLSEHEALSLLRRRARENAPDAVCFLGAGMYDRIIPAAIDAVVQRSEFTTAYTP
                     YQPEVSQGTLQAIYEFQTMVCELFGMDVANASVYDGASALTEAILLSHAHTGRRKVVF
                     AGPVHPRYRSAFSSITVGIGLESVEAPFSDGGADAAAVAERVSGETACVVVQQPNFYG
                     VIEDIAALAAIAHREGALLVVSADPVALSLLEAPGKQGADVVVGEGQPLGIPMSFGGP
                     ALGLFAVRDELKRRLPGRLVGRTVDTRGQQAFVLTLQTREQHIRREKATSNICSNQNL
                     MAIAATMYLTLMGPAGLRTVAENSLQNAHYAADEIEARTVFRRVHARPFFDEFVVKCP
                     VPAREVVDRLVPRGILAGVDMAQFGGDPAHLMIAVTEKRTKEEIDALVAALGEIR"
     gene            complement(1558..1941)
                     /gene="gcvH"
                     /locus_tag="P5179_12415"
     CDS             complement(1558..1941)
                     /gene="gcvH"
                     /locus_tag="P5179_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRS96064.1"
                     /translation="MDFPDDLAYTRDHEWLRLEGETGTAGITDYAQRELTDIVFVEFV
                     APGTVVRQGERFGTVEAIKAVSDLFSPVDGEIVEVNAELSTHPEWVNQDPYGKGWMVK
                     IRVSDPAQVNTLLSAADYRTLVASG"
     gene            complement(1954..3306)
                     /gene="accC"
                     /locus_tag="P5179_12420"
     CDS             complement(1954..3306)
                     /gene="accC"
                     /locus_tag="P5179_12420"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRS96065.1"
                     /translation="MFSKILVANRGEVALRIVRACRELNIATVAVYSEADASSLHVRF
                     ADEAVCIGPPDARQSYLDPARIIAAAEVTGAEAIHPGYGFLAENARFADICAECGFVF
                     IGPTGDMIRMMGDKAVAKKTMADAGVPVVPGSDGPLKSAEQALALAKDHGYPVMLKAV
                     AGGGGKGIRIVTSPEGLVRAYDLARNEAETAFKLGDLYLEKFVDHARHVEIQILGDSE
                     GNVVHLGERDCTVQRRRQKLIEESPSPAVTRDLRDRMASAAVAGAASVGYRNAGTIEF
                     LLAPDRQFYFMEMNTRVQVEHPVTEMVTGTDIVKEQIRIAAGLPLSFRQEDIILQGHA
                     IECRINAEDVAHDFRPTPGRITEWHVPGGIGVRIDTHAYAHYAVPPYYDSMLAKLIVH
                     APTRAEAIVRMKRALGEFFVEGVPTTIPFHSRVMSDSVFQNGHYDMGYVEDFFTRTEI
                     "
     gene            complement(3312..>3497)
                     /locus_tag="P5179_12425"
     CDS             complement(3312..>3497)
                     /locus_tag="P5179_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HRS96066.1"
                     /translation="EAAPYIETGAHVAKGQIIGIIEAMKLMNEIPADVAGTVVEIAVE
                     NAEPVEFGQVLIRVKPD"
BASE COUNT          597 a         1134 c         1108 g          658 t
ORIGIN      
        1 cgtcggtggg gcagaaattc ctgccgcctg ccgggcggcc tgcctgctgc tatcaccgcc
       61 cctgaatctt caccaccact tgatacggcc cgcccgcggt tctgcgcaag gtgcggcggc
      121 tgacgctgtg gggatcgatc ctcgttgcgg ggtgcgccgt acggggtcat gttcgattgc
      181 cggccaccgg ggcagatatt acctgatctc ccccagcgcc gcgaccagcg cgtcgatctc
      241 ctccttcgtg cgcttttcag tgacggcgat catgaggtgt gccgggtcgc cgccaaactg
      301 tgccatatcc acgcctgcaa ggatgccgcg cggaaccagg cggtcgacaa cctcccgcgc
      361 cggaacgggg cacttcacca cgaattcatc gaaaaacggc cgggcgtgca cccggcggaa
      421 caccgtgcgt gcttcgatct cgtccgcggc gtagtgggcg ttctgaaggg agttttcggc
      481 aaccgtgcgc aggccggcgg ggcccatcag ggtgagatac atcgttgccg cgatggccat
      541 caggttctgg ttcgagcaga tgttgctggt ggctttttcc cgccggatgt gctgctcgcg
      601 ggtctgcagg gtcagcacga acgcctgttg cccgcgggta tccaccgtgc ggccgacgag
      661 ccggcccgga aggcggcgct tcagttcatc ccgcaccgcg aacaggccga gggccggtcc
      721 gccgaagctc atggggatgc cgaggggctg cccttcgccc accacgacgt ccgcgccctg
      781 ctttccgggc gcttcaagaa gggacagcgc caccggatcg gcagatacga caaggagcgc
      841 accctcgcga tgcgcgattg ctgcaagggc cgcaatgtcc tcgatgacgc cgtagaaatt
      901 cggctgctgc acgacgacgc acgcggtttc cccgctcacc cgctccgcca ccgcggcagc
      961 gtcggcgccg ccgtcggaaa acggcgcctc aacgctttcc aacccgatac ccacggtgat
     1021 agaagagaag gcagagcggt accgcgggtg caccggcccg gcgaagacaa cctttcggcg
     1081 gcctgtatgc gcgtgggaaa gcagtatcgc ctccgtcagc gccgacgcgc catcgtacac
     1141 cgaggcgttc gccacatcca tgccgaacag ctcgcacacc atcgtctgga actcgtagat
     1201 ggcctgcagg gtgccctggc tcacttccgg ctggtagggc gtgtacgcgg tggtgaattc
     1261 ggagcgctga actactgcgt caatcgcggc cgggatgatg cggtcgtaca tgccggcgcc
     1321 gaggaaacag acggcgtccg gggcattttc ccgcgcgcgc cgacgaagaa ggctgagcgc
     1381 ttcgtgttcg gagagccgtg gaggaagatt aagcgcgctt gccgcacgga tcgcgtccgg
     1441 aacaggggcg acgaggtctt cgaacgagcg gacgccgatg gccgcgagca tttcggcgag
     1501 gtcggaatcg gtgcagggaa tgaacgggtg cggatctgcc atgcacaaac cgccttttca
     1561 gccgcttgcg acgagcgtgc ggtagtcggc ggcggaaagc agggtgttca cctgcgccgg
     1621 atcgctcacc ctgattttca ccatccagcc cttgccgtac gggtcctggt tgacccactc
     1681 ggggtgcgtc gagagctcgg cgttgacctc cacgatctct ccgtccaccg ggctgaacag
     1741 gtcgctgacg gccttgattg cctcgacggt tccgaaccgt tcgccctgcc tgacaacggt
     1801 acccggcgcg acgaattcca caaatacgat gtccgtcagt tcccgttgcg cgtaatcggt
     1861 gatcccggca gtgccggttt ctccttccaa ccgcaaccat tcatgatcgc gggtgtacgc
     1921 cagatcgtcc gggaaatcca cggtcagtcc tcctcagatt tctgttcgcg tgaagaagtc
     1981 ttccacatac cccatatcgt agtgcccgtt ctggaacacc gagtcactca tgacgcgact
     2041 gtggaacggg attgtcgtcg gcacgccttc aacgaagaac tctccgagcg cccgcttcat
     2101 gcgtacgatc gcctccgccc gcgtcggcgc atggacgatg agcttggcca gcatggaatc
     2161 gtagtagggc ggcacggcgt aatgcgcgta cgcgtgggtg tcaatacgga cgccgattcc
     2221 gcccgggaca tgccattcgg tgattcggcc gggcgtcggg cggaagtcgt gcgcgacgtc
     2281 ttccgcgttg atgcggcact cgatcgcgtg cccctggagg atgatatctt cctgccggaa
     2341 actgagcggg agccccgctg cgatgcgaat ctgctccttg acgatatcgg ttcccgtcac
     2401 catctcggta acggggtgct ccacctgaac gcgggtgttc atttccatga agtagaactg
     2461 ccggtcgggc gcgagaagga actcgatggt gccggcgttt ctgtacccga cgctcgcggc
     2521 gcccgccacg gcggcgctgg ccattcggtc gcgcagatcg cgggtaaccg ccggcgaggg
     2581 ggattcctca atcagcttct ggcgccgccg ttgcaccgtg caatcgcgct caccaaggtg
     2641 tacgacgttg ccctctgaat cgccgagtat ctgaatctcg acgtggcggg cgtggtccac
     2701 gaacttctcg aggtagaggt caccgagttt gaacgcagtc tcggcctcat tccgggcaag
     2761 gtcgtacgcc cggacaagac cttccggaga cgtgacgatc cggatgccct ttccgccgcc
     2821 gcccgcgacg gctttcagca tcaccgggta tccgtggtcc tttgcaaggg cgagcgcctg
     2881 ttccgcggat ttcagcgggc cgtcggaacc cggcaccacc ggcaccccgg cgtccgccat
     2941 ggtcttcttg gccacggctt tgtcgcccat catgcggatc atgtccccgg tcggcccgat
     3001 gaagacgaat ccgcattccg cgcagatgtc cgcaaatcgg gcattttctg ccagaaaacc
     3061 gtatccgggg tggatggctt cggccccggt cacctccgcc gcggcaataa tccgggcggg
     3121 atcgaggtaa ctctgccggg catcgggggg gccgatgcag actgcttcat cggcaaaccg
     3181 aacatggagg gaactggcgt ccgcctcgga gtagacggcg accgtcgcga tattgagttc
     3241 tctgcacgcg cggacgatcc gcagcgccac ttcgccgcga ttggcaacca gaatcttgct
     3301 gaacataggc gctagtccgg cttcacgcgg atgagaacct gaccgaattc caccggttcc
     3361 gcgttctcga cggcgatttc gaccacggtt ccggcaacgt ccgccggtat ctcattcatc
     3421 agcttcattg cctcgatgat gccgatgatc tgccctttgg caacgtgcgc gccggtttcg
     3481 atgtacggcg cggcttc
//
LOCUS       DAOTNG010000318         3477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21366_length_3477_cov_4.564874, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000318 DAOTNG010000000
VERSION     DAOTNG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21366_length_3477_cov_4.564874"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5179_12430"
     CDS             <1..200
                     /locus_tag="P5179_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96067.1"
                     /translation="RRVHAAGWCIAAGAPASAVVKSGDQVKKGQVIGRVPDGTLGAHL
                     HASIEGIVTVNEAGIAIQRTK"
     gene            197..748
                     /locus_tag="P5179_12435"
     CDS             197..748
                     /locus_tag="P5179_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRS96068.1"
                     /translation="MSEKAIGLIELSSIASGFQTADAMLKAANVRLLLSRSICSGKYM
                     VLVGGEVGAVQSAVEAGAASADGCLVDRFIIPGVHEDVFAALGRSTIPTIGESLGVLE
                     AFSVASLIEGADAAAKAARVTLVEIRLAMALGGKAFVTMTGDVASVQAAVEAGKARIA
                     EAGMLVNAVVIPRPHEDVYRETV"
     gene            884..1558
                     /locus_tag="P5179_12440"
     CDS             884..1558
                     /locus_tag="P5179_12440"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRS96069.1"
                     /translation="MQKENVALDNGTGRHVSVKICGIRTRHDALLAADLGATYVGFVF
                     VRKSPRYIDPAEAAEIVPALPRGVVPVGVFANQPRDRVLDIISATGVKVAQFSGLESP
                     EYCDSMGRFGVFKAFHVRPPFSFENLEPYHVNMFVLDTYVPGKNGGTGETFDWSLAIP
                     VARKTRVMLAGGLTPANVVEAVETVRPYAVDVSSGVESSPGEKSPHLMETFFQSLHDA
                     GYQTSR"
     gene            1986..2411
                     /locus_tag="P5179_12445"
     CDS             1986..2411
                     /locus_tag="P5179_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96070.1"
                     /translation="MPEHTSAWKRLRESVRYALAMPPPLELAPADREVLAKVASMLVR
                     RRLGSPALMVLEMSRPINFVASQFLVFMKPFATALLNPTEYERFVRILEHREGIDALV
                     EAIANGNENGAATVQKRQPSPLASSVSCRDNKTHVESKG"
     gene            2429..>3477
                     /locus_tag="P5179_12450"
     CDS             2429..>3477
                     /locus_tag="P5179_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRS96071.1"
                     /translation="MQQERDLNVVIATDCGSTTTKAILIEKVDGTYRQTFRGEAPTTV
                     EAPFEDVTRGVLNAIQEVEELSGRKILEGEHIITPAEGNRGVDIYISTSSAGGGLQMM
                     VTGVVKSMTGESAQRCALGAGAIVMDVLASNDGRLPHEKIERIRQLRPDMVLQSGGTD
                     GGTVSHVVELAEFIAAADPKPRFGTGFKLPVIYAGNKNAIDDITRILGEKTALTVTEN
                     IRPTLEKENLMPARHKIHDLFLEHVMAQAPGYRKLISWCGADIMPTPGAVGLIMQTIA
                     RKQGINVVGVDIGGATTDVFSVFDEVFNRTVSANLGMSYSISNVLAEAGLENIARWIP
                     FDIDEGDLRNRIKNK"
BASE COUNT          751 a          953 c         1078 g          695 t
ORIGIN      
        1 cccgacgggt tcacgccgct ggatggtgca tcgcggccgg tgcaccagca tcggctgtgg
       61 tgaagtccgg cgaccaggtg aaaaagggcc aggtgatcgg gcgcgtaccc gacggcactc
      121 tcggcgcaca cttacacgct tccattgagg gaatagtgac cgtcaacgag gcaggtattg
      181 ccattcagag gacaaaatga gtgagaaagc gatcgggttg atagagctct ccagtatcgc
      241 gtccggcttt caaacggcgg acgcgatgct gaaggccgca aatgtgcgcc tcttgctttc
      301 ccgcagcatt tgttcgggta aatacatggt tcttgtcggg ggcgaagtgg gggcagtgca
      361 aagcgcggta gaagcggggg cggcgtctgc cgatggctgt ctggtggaca ggttcattat
      421 tcccggcgtg cacgaagacg tgtttgcggc gctgggccgc tcgacgatcc cgacgattgg
      481 cgaatcgctt ggagttctcg aggcgttcag cgtcgcttcg ctgatcgagg gggccgacgc
      541 tgccgcaaag gctgcgagag ttacccttgt ggaaatcagg ttggccatgg cgctgggagg
      601 gaaagccttt gtcactatga cgggtgatgt cgcctccgtg caggcggcgg tggaagcggg
      661 caaggctcgc atcgccgaag caggcatgct ggtgaatgca gtggttatcc cacgccccca
      721 cgaggacgtc taccgcgaaa ccgtgtgacg aagtcgttag tggcgagcat ctccacgctc
      781 tcgggtaccg cagcccaggt ctgcggattt atctatttgc gagagaatac gatagcgcgt
      841 ttcatttcca aaggcacacg atcgggaaag tgagcagcag agtatgcaga aggagaatgt
      901 cgccctggac aacgggacag gcaggcacgt ctcagtgaaa atctgcggga tccgcacccg
      961 tcacgatgca ttgctcgccg ctgatctcgg cgccacctat gtggggtttg tgtttgtcag
     1021 gaaaagtccg cgctacatcg acccggcgga agccgcagaa atcgtgccag cgttaccccg
     1081 gggtgttgtt ccggtagggg tttttgccaa tcaaccgaga gacagggtgc tcgacataat
     1141 atcggcgacg ggtgtcaaag tagcgcaatt cagcggtctg gagtccccgg agtactgtga
     1201 cagcatgggc agattcgggg tgttcaaagc gtttcacgtt cggccgccgt tttcctttga
     1261 gaacctggaa ccgtaccacg tcaacatgtt cgttctcgac acgtacgttc cgggcaaaaa
     1321 tggagggacg ggcgaaactt ttgactggtc tctcgcgatc ccagtcgcca ggaagacacg
     1381 tgtgatgctg gcgggcggac tcactccggc aaacgttgtc gaggcggtag aaaccgtccg
     1441 cccttacgcg gtggacgtga gcagcggcgt cgaatccagt ccgggcgaaa aaagtcctca
     1501 cctcatggaa acgttcttcc agagcctgca tgacgcgggc taccagacca gtcgctgaca
     1561 gccggccgtg atgcctgtca acgcccatcc tcctgcgtca cctccagaag acgcaatcac
     1621 gtttttctct catccgtggc gcgaggatgc cccgttccgg cgatggtcac tcgtaagcgc
     1681 catggtcgcg gcatcagcgg tcgccggttt cgggttcaca agcccgggta tcggcctcgg
     1741 agccttcggg tttctttttc tgagcctttc tcggtatttt ttacctacct tttacgaaat
     1801 caatccgcgc ggcgtgcatg cccgacacct cggggtgact cgattccacc cctgggcgcg
     1861 cttcaaacgt gtcgcgttgc gcagggacgg tgttttcctg ggtacttttc cgcggccgag
     1921 acatctggat gcgtggcgcg gactgtatgt tcgcacctcc ggaaaccgag agcaggtata
     1981 cgcctttgcc agagcacaca tccgcctgga aacgcctccg cgagagcgtc agatatgccc
     2041 tggcaatgcc tcccccgctt gagttggctc ccgcggatag agaagtcctt gcgaaagtag
     2101 cctccatgct cgtgagacgg agactcggtt ctcccgcact catggttctg gagatgtccc
     2161 ggccgatcaa cttcgtcgca agccagttcc tggtgttcat gaaacccttt gccacggcgc
     2221 tgttgaaccc gacggagtac gaacgttttg tgcgcatact ggaacaccgg gagggcattg
     2281 acgcgctcgt ggaggcaatt gctaacggca acgagaacgg ggcagcgacc gtgcagaaaa
     2341 ggcaaccgtc tccgctggcc tcgagcgtca gttgccgtga caacaaaact cacgtcgaat
     2401 caaaaggttg aaaggcgcaa aggaatttat gcagcaggaa cgggacctga acgttgttat
     2461 cgcaacagac tgcggcagca ctacgacgaa agcaatcctt atcgagaagg tggacggcac
     2521 ctaccggcag acattccgcg gggaggctcc gactaccgtc gaggccccgt ttgaagacgt
     2581 tacgagaggc gttctaaacg cgatccagga agtggaagag ctgagcggaa ggaaaatact
     2641 cgaaggggaa cacatcatca ctcccgcaga agggaaccgt ggtgtcgaca tctatatctc
     2701 gacatcgtct gctggcggtg gattgcagat gatggtcacg ggggtggtga agtccatgac
     2761 gggtgagagt gctcaacggt gtgcgcttgg tgctggcgcc atcgtcatgg acgtacttgc
     2821 gtcgaacgac ggcaggctcc ctcacgagaa gattgaacgg atccgtcagc tacgccccga
     2881 tatggtgctg cagagcggcg gaacggacgg tgggactgtc tcacacgtgg tggaacttgc
     2941 ggagttcatc gcggccgcgg atcccaagcc gcgtttcggg accggattca agctccccgt
     3001 gatatacgcg ggaaacaaga acgcgatcga tgatatcaca cgaattctcg gggagaaaac
     3061 cgcacttacc gtcaccgaaa acatccgtcc gacgctggaa aaagagaacc tcatgccggc
     3121 gcggcacaaa atccacgacc tgtttctcga acacgtcatg gcccaggcac cgggctacag
     3181 aaagctgatt tcgtggtgcg gagccgacat catgccgacc cccggtgcgg taggcctgat
     3241 catgcagaca atcgcccgca agcaaggcat caatgtggtc ggtgtggata tcggcggcgc
     3301 cacgacagac gtgttcagcg tgtttgacga agtgttcaac cgcacggtga gcgcgaatct
     3361 cggtatgtcg tacagcattt ccaatgttct ggccgaggcc ggactcgaga acatcgcgcg
     3421 ttggataccg tttgatatag atgaaggtga cttgcgtaac agaatcaaaa acaagat
//
LOCUS       DAOTNG010000319         3456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21522_length_3456_cov_3.605998, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000319 DAOTNG010000000
VERSION     DAOTNG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21522_length_3456_cov_3.605998"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="P5179_12455"
     CDS             complement(<1..526)
                     /locus_tag="P5179_12455"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRS96072.1"
                     /translation="MANFPAVRSWRAALTAPSPALINRVARMRNQSPSEAGSAIRWYA
                     TLVERARSVYGHNDDVPMFIVRAPGRVNLLGMHIDHRGGRVNPIAVRELVLVCVPRSD
                     NRIRIANMNPSFPADEFAVRELLPENKITDWADWTLTTPETLRKKGLLGHWSSYVRSA
                     AAYFANSWGMRDSIR"
     gene            complement(603..1046)
                     /locus_tag="P5179_12460"
     CDS             complement(603..1046)
                     /locus_tag="P5179_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HRS96073.1"
                     /translation="MSALKGIPMSISPELLEVLARMGHGDHIVLADGNFPGESQGADY
                     VIRVDGMGVPALLKDLLKLMPVDTGESSAYVMAVPKGQPEPPIWAKYREIINKAEKRE
                     VKLVELDRFAFYDFTKEKAFAIVMTGETALYANLILVKGVVAPKK"
     gene            1271..2467
                     /locus_tag="P5179_12465"
     CDS             1271..2467
                     /locus_tag="P5179_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96074.1"
                     /translation="MTGLIVLFALWVVVLTGVWVHPRLSRKAKTIALVPIVAYGIVSG
                     VSVARSYAAGRPQFSRVTQKWALAEEHLASVLGSAPDSALAKPLVQSAKKLHADLKRT
                     MRFGVWSWGVRYRFGIIPQVNGDVVAANLERLLDVPAQLVYAEAANVRNSRDFTQCYD
                     YRLLVTRDFPMNYSRSAGVVSMILEAYKSGATTAIGLCDTVNLARDDSRDPRSPTYRL
                     SAVAVKLVGMQNKLDELLAVACGAFDEEIALSVRNVRPPDISQSPEWKPLSPLFMDFT
                     PLVPNLTGISTIEVRWVGDPPKPVVGTTEPMYRLDCETRGTWPAGQDIWRDSTGSSGP
                     TYFVDASASRVFAKGVRLLKWDRQPDIRSLNIMANAEDRRYAGIPTVDVTEDLFLPPP
                     DRSKRR"
     gene            2464..3309
                     /locus_tag="P5179_12470"
     CDS             2464..3309
                     /locus_tag="P5179_12470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRS96075.1"
                     /translation="MSTGRHTCVIRSRLKEISFDHIYFGTLFTWFEVARTEMGRAAGL
                     PYVELEKNRIGSFVTAAGAQYHRPIPAGATVAVEARMAEMGRARCSFAYAVFTGNDES
                     PAVTGYTDHIAADFSGRPTRLPACFAEAFAPTGESIPRPSLEEPARVHWNTDLRVRYE
                     ETDAFGVVYNGNYFAWMEASWSEHLAENGMDVVEAFRQGRTYPVVEASCRFLSPLRYD
                     DPVSIEVESRLEGRTRVALRYRFVAASTGVTAGIGQMVHAVVNKGRVVPVPRELARAL
                     GITDS"
BASE COUNT          690 a          975 c         1061 g          730 t
ORIGIN      
        1 ctcgaatcga atcgcgcata ccccatgaat tcgcaaaata cgccgctgcc gatcttacat
       61 acgagctcca atgaccgaga agccccttct tccggagtgt ttccggcgtg gtgagcgtcc
      121 agtccgccca atcggtgatc ttattctcgg gcaggagctc ccgcaccgcg aactcgtccg
      181 cgggaaacga cgggttcatg ttggctatgc ggatccggtt gtcggacctt ggaacgcaga
      241 caagcaccag ttccctgact gcgatcggat tgacccggcc gcctcgatgg tctatgtgca
      301 tgccgagaag attgacccgc cccggagcgc gaacgatgaa catcgggacg tcatcgttgt
      361 ggccgtatac tgaccgcgca cgttccacaa gtgtggcgta ccaacggatc gcgcttcccg
      421 cctcggacgg agactggttg cgcatgcgtg cgacgcggtt gatcaacgcg ggggagggcg
      481 cagtaagggc agcgcgccag gaacggacag cagggaagtt cgccatctgg cgcttccttc
      541 agaaagaagt gagcaggggc agccttcgcg gccgcccctg acgaaaacac cggctcggat
      601 tgctatttct tcggcgctac cacgcccttc acgaggataa ggttcgcgta aagggctgtt
      661 tcgcccgtca ttactatcgc gaaggctttc tctttcgtga agtcatagaa tgcgaaacgg
      721 tccagttcga caagtttcac ttcgcgtttt tcggctttgt tgatgatttc gcggtacttt
      781 gcccatatcg ggggttccgg ttggcctttg ggcaccgcca tcacgtatgc tgagctttcg
      841 cctgtatcca ccggcatcag cttcaggagg tccttgagca gggcaggaac acccattcca
      901 tccactcgga tcacgtaatc agccccctgg ctttccccgg gaaagttccc atcggcgagt
      961 acgatgtggt cgccgtggcc catgcgggcg agaacctcca acaattcggg ggagatgctc
     1021 atcggaatac ccttgagcgc agacatggta accatccttt cgtttctgga agtggccaca
     1081 caaccgacgg gttgatcagg atatgcattc ccttttgtca ggtcgaatac taatgatatg
     1141 cgcagacagg ggtcgaattc aagcgtttcg gctagggttt ctccggtgtc tccgcttaca
     1201 ttttctgatg cgaaacacac cgacgtgtcc gcatcgcctg acattctgac ttccgcggag
     1261 atcgtgctcc atgacaggct tgatcgttct gttcgcgctc tgggttgtgg tgttgacagg
     1321 ggtgtgggtt cacccgagac tcagccgcaa ggcgaaaacc atcgcactcg tgccaattgt
     1381 tgcgtacggg attgtcagtg gagtaagtgt cgcgcgttcg tatgcagccg gccgcccgca
     1441 gttctcccgc gtgactcaga aatgggcgct tgccgaggag cacctcgcgt ctgtgctggg
     1501 cagcgcgccc gactcggcac ttgccaagcc gctcgtccaa tcggccaaga agcttcatgc
     1561 agacctgaaa cgcaccatgc gtttcggcgt gtggagctgg ggagtacgat acaggttcgg
     1621 gataatccct caggtcaatg gcgatgtggt ggccgcaaat ctggagaggc tgctcgatgt
     1681 gccggctcag ctcgtgtatg cggaagcggc caatgttcgg aattcacggg acttcactca
     1741 gtgctacgac taccgacttc tcgtaactcg cgatttcccg atgaattaca gccgaagcgc
     1801 gggcgtcgtt tcgatgatac ttgaagcgta caagagtggg gcaaccactg ccatcggcct
     1861 gtgcgacacc gtgaacctcg cgagggacga ttcgcgcgac ccgcgaagtc ccacctaccg
     1921 actttcggcc gtcgccgtaa aacttgtggg gatgcagaac aaactggacg agcttctggc
     1981 cgtagcgtgt ggagccttcg acgaggaaat tgctctttct gttcgcaatg ttcgaccgcc
     2041 ggacatttcc caaagcccgg agtggaagcc cctcagcccc ttgttcatgg acttcacgcc
     2101 gctggtgccg aatctgacgg gcatcagcac gatcgaggtg cgatgggtgg gagacccgcc
     2161 gaaaccggtg gtaggaacga cggaaccgat gtacagactg gattgcgaaa cccgggggac
     2221 gtggcccgca ggacaggata tctggcgcga ttcgacgggc agttcgggac cgacgtattt
     2281 cgttgacgca tctgcaagcc gggtttttgc gaaaggcgta cgcctgctta agtgggatcg
     2341 ccagccggac atcagatctc tgaacatcat ggcaaatgcg gaggaccggc ggtacgcggg
     2401 tattcccacc gttgacgtca ctgaggacct ttttcttccg ccacccgaca ggtcaaaacg
     2461 gagatgagta cgggaaggca tacgtgtgta atccggagtc ggctgaaaga gatatccttc
     2521 gaccacatct acttcgggac gttgttcacc tggtttgagg tggcacgtac cgaaatggga
     2581 cgcgccgcag ggctgcccta cgtggagctc gaaaaaaacc ggataggctc gttcgtcact
     2641 gctgccggcg cgcagtatca cagaccgatt cctgcaggcg cgacggtcgc ggttgaggcg
     2701 cgaatggcgg aaatggggcg ggcgaggtgc tccttcgcgt acgcggtgtt cacaggaaac
     2761 gatgagtccc cggccgtgac gggttatacc gaccacatcg cggcggattt ctcgggcagg
     2821 ccgacgcgtc tgcccgcgtg tttcgcggag gcgtttgcac cgactggcga gtcgatacct
     2881 cgtccctcgc tcgaggaacc cgcccgggta cactggaaca ctgatctccg tgtacgttac
     2941 gaggagactg atgcgttcgg agtggtgtac aacgggaatt acttcgcctg gatggaagcg
     3001 tcgtggagcg agcatctcgc ggaaaacggc atggatgtcg tcgaagcctt ccgacagggg
     3061 cggacgtacc cggtcgtgga agcctcgtgc aggtttcttt ccccgttgcg gtatgacgat
     3121 cctgtgtcaa tcgaggtaga aagccggttg gaaggccgga cccgcgtggc actgcggtac
     3181 cgatttgtcg cagcatctac aggagttacc gcgggtatcg gtcagatggt acacgccgtc
     3241 gtgaacaaag ggcgtgtggt tcctgtgccc cgtgagctgg ctcgcgcgct gggaataaca
     3301 gactcctgat tccggtggaa acgaacggtg tgttcgtggt tcagtcgtcg ccggactcca
     3361 taggctcgcg ggattcccac tccgcccggg gaaccgaaac aatgaccatt gtctgcggca
     3421 agacgaggta gtgttcctga tcgaacacca cgatgc
//
LOCUS       DAOTNG010000320         3444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21618_length_3444_cov_4.241369, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000320 DAOTNG010000000
VERSION     DAOTNG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21618_length_3444_cov_4.241369"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="P5179_12475"
     CDS             complement(<1..920)
                     /locus_tag="P5179_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS96076.1"
                     /translation="MVSVQLEDVTKTFGSARAVDRVTLNIARGELFFLLGPSGCGKTT
                     LLRMIAGFYEVDEGRILFDGRDMTGVPPHKRNTGMVFQNYALWPHLSVAENVAYGLEV
                     RKVPVTEKQDRVMRALEMVRMDSLAGRSPNQLSGGQQQRVALARALVIEPEAVLLDEP
                     LSNLDAKLRLEMREEIRRIHDELGLTMIYVTHDQKEALSMADRLAVMNRGRIEQTGAP
                     REVYVTPENVFVAGFIGETNLFPGMLLPDGSSGAEQQVDTPYGVLRVARKSSTAEPGK
                     VSVSIRPEAISVRGAEEALSGGILRGVVQS"
     gene            complement(952..3222)
                     /locus_tag="P5179_12480"
     CDS             complement(952..3222)
                     /locus_tag="P5179_12480"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRS96077.1"
                     /translation="MADASASQPLPRHDLLYERASDEWNTGAPLGNGQLGVMVWGDGN
                     PLRLTLDRDDLWDLRVPSGPDDPSFTWRNLKKLAQEEDWETLNATVERNLNPPAPTPT
                     KLPLGRLEIEFRGTSSNANGFRGRLSLKKAEFSATFGGNRVKVFVCAHAPVVVVECYG
                     ESASPLVFWRSFRDLNPGQAASLGIQPCDSLQIYDITAAVQRFPDGTTAVVAWHCAPS
                     AGGFVVRCTVATSADGDDPVSVAADRLRAAASTYSLKRRHREWWSDFWSRSSITIPEK
                     RLETLWYYGLYKLASSSRPGNLPANLQGLWVTDGVLPPWRGDYHANMNIQETYWPVYA
                     TNHLEIGLPLLQWIESITEPARQRTRSFFGWDGVRIETALAPNGVPVPGWGTVQYWPG
                     AATWLAHHLWLHWRYSGDRNFLREKAFPFFQLCFAFWEGYLEKGTDGYLHVPLSHSPE
                     WNGNLPEAWGRDPVIDLSLIRNLCLWLDESARELRVSGEMQTRWKVVAESLAPYPRDP
                     AGGLMLMEGVPLSESHRHPSHLMPVFPMHDMSAEGTEEDRKCIEVSLAQLERKGMGEW
                     TGWSFPYVSLIASRVGRACMAEYMLKLYIESFVLPNGCHANGDWRGNGICQYHYSPFT
                     MEAECAATSAVTEMMLQSWGGTIRLFPAVPETWLDASFHGFLAEGGIEVSAVREFGET
                     TEVTLRSRDRAEARVVGLKGNAQWRGAASARWETGTWVVTLDAGVPACGRCHRRKTGK
                     RRATESNTRNIFGLRI"
BASE COUNT          713 a         1091 c          919 g          721 t
ORIGIN      
        1 atggattgga caacgccgcg cagaattccc cctgacaagg cttcctccgc ccctctgacg
       61 ctgatcgcct ccggccggat tgatactgat actttgcccg gttccgcggt actgctcttt
      121 ctcgccaccc gaaggacgcc gtaaggcgtg tccacctgct gttctgcacc tgaggacccg
      181 tcgggcaaga gcatgccggg gaaaagattg gtttccccga tgaagcccgc aacaaagacg
      241 ttttccggtg tcacgtacac ctcacgtggc gctcccgttt gctcgattcg tccgcgattc
      301 atcacggcga gtcgatcagc catggacaac gcttctttct ggtcgtgcgt gacataaatc
      361 atagtcaagc ccaactcgtc gtgtattctg cgaatctcct cgcgcatttc gagcctgagt
      421 ttggcatcca gattggaaag cggttcgtcc agaagcactg cttcgggttc aattaccaga
      481 gccctcgcaa gagccacgcg ctgctgttgc cctccggaca attgattcgg cgagcggcca
      541 gcgagggaat ccatcctcac catttccagc gccctcatga cccgatcttg tttctccgtt
      601 acgggtacct tccgaacctc gagtccgtac gccacgttct ccgccaccga gagatgtggc
      661 cacagcgcgt aattctggaa caccataccc gtgttccgct tgtgaggagg cacgcctgtc
      721 atgtctcgac cgtcgaacag aatgcgcccc tcgtccactt cgtagaaacc tgcgatcatc
      781 cgtagaagcg tcgttttccc gcagcccgaa ggtccaagga gaaaaaacaa ctcgccgcgg
      841 gcaatattca gggtcacccg atcgacggcg cgggcgctcc cgaaagtttt cgtcacatct
      901 tcaagttgaa cacttaccat cctttgactc cgcgtgaaaa ccgtgtcatt ctcagattcg
      961 aaggccaaat atgttccgcg tattcgattc ggtcgccctt cgcttgcccg ttttccggcg
     1021 gtggcaccgc ccgcacgcag gaacgcccgc atccagcgta acaacccacg ttccggtttc
     1081 ccagcgtgct gaggcagctc cccgccattg ggcatttccc ttcagaccca caaccctcgc
     1141 ctccgcgcga tcccgtgaac gaagtgttac ctccgtagtc tccccgaact cccgaaccgc
     1201 acttacttcg attccaccct ctgcgagaaa cccatgaaat gacgcgtcaa gccaggtttc
     1261 ggggaccgct ggaaacaatc tgatcgtccc gccccatgat tgaagcatca tttcggtaac
     1321 cgcagatgtt gcggcgcatt ccgcttccat ggtgaacggt gagtagtgat actgacagat
     1381 tccgtttccg cgccaatcac cgtttgcgtg gcacccgttc ggcaatacga acgattcgat
     1441 gtacagcttc agcatgtact ccgccatgca ggctcgcccg acccggctcg caatgaggga
     1501 gacgtagggg aatgaccagc cggtccattc ccccatgccc tttcgctcca gttgtgccag
     1561 tgaaacctcg atacatttcc tgtcttcctc ggtaccttcc gctgacatat cgtgcatggg
     1621 aaacacaggc attagatgcg aagggtgacg gtggctctcg gagagcggca cgccctccat
     1681 cagcatcaaa ccgcctgccg gatcgcgtgg atacggtgcc agggactccg cgacaacctt
     1741 ccagcgggtt tgcatctcgc cactcacgcg cagttcgcgc gcgctctcat ccaaccagag
     1801 acagagattg cggatgaggg aaagatcgat caccggatct cgaccccatg cctccggcag
     1861 gttgccgttc cactccggcg agtgagacag cggaacatgc agatacccgt cggtcccctt
     1921 ctccagatac ccttcccaga atgcgaagca caactggaaa aaggggaacg ccttttcacg
     1981 aaggaagttc cggtctcccg agtatcgcca gtgaagccac aggtggtgtg caagccatgt
     2041 tgctgctccc ggccagtact ggacagttcc ccagcccgga accggcaccc cgttgggtgc
     2101 gagggccgtt tctatccgaa cgccatccca cccgaagaaa cttcgtgttc tttggcgcgc
     2161 tggttcggtg atactttcaa tccactggag caaagggagg cctatttcca ggtgatttgt
     2221 ggcatacacc ggccagtacg tttcctgaat gttcatgttg gcgtggtaat cgccgcgcca
     2281 gggcggcagg actccgtcgg ttacccagag accctggagg ttggccggca ggtttcccgg
     2341 gcgggatgat gacgcgagtt tgtaaagccc gtaataccag agcgtttcga gcctcttttc
     2401 cggaatcgta atgctggaac gagaccagaa atcactccac cactcccggt ggcgacgctt
     2461 gagagaatac gtactcgcgg ccgcgcgcag cctgtctgca gcgacagaga cgggatcatc
     2521 gccgtcagca ctggttgcaa cggtgcaccg tactacaaat cctcctgcgg atggcgcaca
     2581 atgccatgca acgactgccg ttgtgccgtc cgggaaacgt tgtacggcgg cagtaatatc
     2641 atatatctga agcgagtcgc agggctggat tccaagggag gccgcctggc ccggattgag
     2701 atcccggaac gaccgccaga acacaagagg ggatgccgac tcaccgtaac attcgaccac
     2761 caccaccggc gcgtgggcac acacaaaaac cttcactctg ttcccaccga aggtcgcact
     2821 gaactctgcc tttttcagag agagcctgcc acggaaaccg ttggcattcg aactggttcc
     2881 ccgaaactca atttcgagcc ttcccagagg caacttggtc ggagttggag ccggaggatt
     2941 caggttcctt tccaccgtcg cattcagagt ttcccaatcc tcttcctgtg caagcttctt
     3001 caggttccgc cacgtgaacg acggatcgtc aggcccggaa ggcacgcgca gatcccaaag
     3061 atcgtcgcga tcgagagtga ggcggagagg atttccatcc ccccagacca ttacgccgag
     3121 ttggccgtta ccgagaggag caccggtatt ccactcgtct gaggctcgtt cgtagagcag
     3181 atcatgtctg ggcaaaggtt gagaagcact cgcgtctgcc atgaaatccc cgcaagtcgt
     3241 atgtctgtga gaagccttca ctccttggaa agacggccgc gcgccgcccc taaccccgca
     3301 ggccgaagac atttcgcggc tgaggttctc tggtattggc gtttggcgat ttccgcgcaa
     3361 tctgccgtgc ggtcgcggga gtcgcaggcc gcagagccac cgaccagagt ccgtggttcc
     3421 agatcgcgct tctcacgcca gtcc
//
LOCUS       DAOTNG010000321         3429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21753_length_3429_cov_5.537937, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000321 DAOTNG010000000
VERSION     DAOTNG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21753_length_3429_cov_5.537937"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1874
                     /locus_tag="P5179_12485"
     CDS             <1..1874
                     /locus_tag="P5179_12485"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRS96078.1"
                     /translation="HVVTDERENVAAIARFFTGSSENAGQIRRTGTVGEVSITRNHEI
                     LVTIGKEGVETGRLAEAVLKDPRRIREFDRGATIVIPADAMKDGYRAAVLTHLFFSDV
                     IREDSRRHIVTLPIESLSRVSLWFTGTGENWPLLKRASGKRSNDVAKGEVIVIPRNLL
                     GSWALMTEEDISEETRDYPLALTSRSRRDAVNLGQRLLIPKRLLSAWTALMEKGEDGA
                     QDAAKWYVYWGTRNPPLVYSGDRLGGYAGYRLQRGQALYSDVVIRFTGVVEVAEVLHL
                     SEVVLRRSGFRDPRKLPVNALIKIPIGLLSPEWRPEGDPARLAEREEDTAVARVEEEI
                     RVEKAEARAAEPVRPRGEALREVTIVLDAGHGGHDPGAIGPRGLTENEVVYDIMCRVM
                     RQFAQRTQATVYVTIEDARTGHTPSERATIPDNREEHLLTTPKYPNADVVTSANLRWY
                     LANSYVRRAINTGTDLERVVFTSFHADALHPSMQGCMVYVPSARHSKGRYSAGTRYAI
                     YHEVSERPTVSYSYNERLRSQALSSGFADALCQRLRSANVGVHGRKPVRGYIIRRSGG
                     GEWVPAILKYNAAPTKILVEIGNIRNENDAANMRDPAWRERFASAYVDAVISHFSTQ"
     gene            1884..2963
                     /locus_tag="P5179_12490"
     CDS             1884..2963
                     /locus_tag="P5179_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96079.1"
                     /translation="MNVNIEPYLMRLNDVFDPERSSKSVERQKNVCLFHKSDRLPVAF
                     VLDEVPGWERIRLDEAYACPELMLLNELRPIYGSALVGDDRANTIRANFGVGILPSLF
                     GCEIKLTYDAMPWVEPLNNREAVAKIVSAGALDLRGGLWARIEEFQEFAIAQLADFPA
                     LRSGIRLSMCDMQGPFSIAHLVWGNDIYYAVVDDPDLVRDFLELVTDVYIRFVRQERE
                     ATKADEEFTEFLNVLVPGKILIREDSATNLSPAMYEEFCLPYIQKTLDAFPGGVHYCG
                     AGHQFFELVAGCRNLTAMNFGNPEMQRPERVRSLAARGIAVVGWGLWPLDHPLDELET
                     GISLGISVHDVDEGKRIMDQVASRG"
     gene            complement(2975..>3429)
                     /locus_tag="P5179_12495"
     CDS             complement(2975..>3429)
                     /locus_tag="P5179_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HRS96080.1"
                     /translation="TLGAFTWGIEQVLAYTWFLSNVLYFSIPEGAFRVVLALLVLCTC
                     AAGFAAFRDPEGIRIRLWLACNGFWLAVALCTILFAIRFRGYVPGLFTSCIVVIPGSA
                     FALWRAYAARFLPPDNVRTWTIAGAAAFLPLVVVLTGAGWLAFELCTP"
BASE COUNT          765 a          937 c         1057 g          670 t
ORIGIN      
        1 aacatgtggt aaccgacgag cgcgagaatg tcgcggcaat cgcccggttt ttcactggtt
       61 cgtcggaaaa cgccgggcag atacgccgaa ccgggacagt cggcgaagtc tccatcactc
      121 ggaaccacga aatcctcgtg acgatcggca aagaaggtgt cgaaacgggc cgtcttgccg
      181 aagctgtcct gaaagacccg cgtcgtatcc gtgagtttga caggggtgcg acgattgtaa
      241 ttcccgcgga tgcgatgaaa gacggatatc gagccgccgt tctgacacat ctgtttttct
      301 ctgacgtgat ccgggaagac tcgcgacggc acattgtcac tctcccgata gaaagccttt
      361 ctcgggtctc gctctggttt acgggaaccg gcgaaaactg gcccctcctt aagcgcgctt
      421 cggggaaacg ctcaaatgat gtggcgaagg gtgaagtcat tgtcatcccg cgaaatctgc
      481 tgggaagctg ggcccttatg acggaggagg acatttccga ggaaaccagg gattaccctc
      541 tcgccctcac ctcccgtagc aggcgggatg cggtcaacct cggccagcga cttctgattc
      601 cgaagcgact gctttctgcg tggaccgccc tgatggaaaa gggagaggac ggggcacagg
      661 atgcggcaaa atggtacgtc tactggggga cgagaaatcc gccccttgtg tattcggggg
      721 accggctggg cggttatgcg gggtaccgtc tgcagcgagg ccaggcgctg tactccgacg
      781 ttgtcatccg cttcacgggg gtggtcgaag tcgcggaggt cctgcacctc tccgaggtcg
      841 ttcttcgcag gtccggtttc agggatccac gcaaacttcc ggttaacgcc cttatcaaga
      901 ttcccatcgg gttgttaagc cccgagtgga ggcctgaagg cgaccccgcg cggcttgccg
      961 agcgtgagga agacacggcg gttgcccgcg tggaagagga aatccgcgtc gaaaaggcgg
     1021 aggcacgcgc tgcagagccg gtccgcccac gtggcgaggc gctacgcgaa gttacgatcg
     1081 ttcttgatgc gggtcacggg gggcacgacc cgggcgctat cggaccgcga ggtctgacgg
     1141 aaaacgaagt ggtgtacgat ataatgtgtc gtgtgatgag gcaatttgcc caacgaacac
     1201 aggcaaccgt gtacgtaacc attgaggacg cacggacggg gcatacgcct tcggagcggg
     1261 ctacaatacc ggacaatcgg gaggagcacc tcctcaccac gccgaaatac cccaacgcgg
     1321 acgttgtcac atcagccaat cttcgatggt acctggcgaa ctcctacgtc aggcgcgcga
     1381 tcaatacggg tacggacctc gagcgggttg tgttcacgag ttttcacgcg gacgcgctcc
     1441 accccagtat gcagggatgt atggtgtacg ttccgagcgc ccgccacagc aagggacggt
     1501 acagcgcggg aacgcggtac gcgatctacc acgaagtatc ggagcgtcct accgtgagtt
     1561 actcgtacaa cgaacggctc cgttcccagg cgttgtcttc ggggtttgct gatgcgcttt
     1621 gccaacgtct cagaagcgcg aacgtagggg ttcacgggcg caaacctgta cgcgggtaca
     1681 tcatacggcg aagcggcggt ggcgaatggg ttccggcgat actcaaatac aatgccgcgc
     1741 ccaccaaaat tctagtggaa atcgggaaca tcaggaacga aaacgacgct gccaacatga
     1801 gagatcctgc atggcgggag cggttcgctt ccgcgtacgt tgacgcggtg atttcgcatt
     1861 tctctaccca gtgagaaaaa agcatgaacg tcaatatcga accctacctc atgcgcttga
     1921 acgacgtctt tgatcctgag cggagttcaa aatcggtcga acgccagaaa aacgtgtgcc
     1981 ttttccacaa atcggaccgg ttgccggtag cattcgtgct ggatgaagtg ccaggctggg
     2041 aacgaatcag gctggacgag gcgtacgcgt gtcctgaact gatgttgctc aacgaactcc
     2101 gtcccatcta cggtagtgcg ctcgtaggtg atgatcgcgc gaatacgatt cgagcaaatt
     2161 tcggtgttgg gatactcccg tctctttttg ggtgcgagat caaactcacg tatgacgcca
     2221 tgccgtgggt agaaccgctg aacaacagag aagcagttgc gaagattgtc agcgccggcg
     2281 ccctggattt gcgtggaggc ctctgggcga ggatagagga gtttcaggag tttgcgattg
     2341 cacagcttgc cgatttccct gctctgcgtt ccgggattcg gctttccatg tgcgacatgc
     2401 agggaccgtt cagcattgcg cacctggtgt gggggaacga catctactac gccgtagtgg
     2461 acgaccccga cctagtgcgg gatttccttg aattggtcac cgacgtgtac atccgttttg
     2521 tccgtcagga acgagaagcg acaaaagccg acgaagagtt cacggagttt ctcaatgtac
     2581 tggttcccgg caagattctt attcgagaag actcggcaac aaacctctct cccgcgatgt
     2641 acgaggagtt ttgtcttccg tacatccaga agacgcttga cgcgtttccg ggaggcgttc
     2701 attactgtgg cgcgggacac caattcttcg aactggtagc cggatgccgg aatctgaccg
     2761 cgatgaactt cggaaacccg gaaatgcaac gtcctgaacg ggtcaggtct ctcgcggcgc
     2821 ggggcattgc agtggtggga tgggggctat ggccgctcga tcatccgttg gacgaactgg
     2881 aaaccggtat cagcctgggc atctccgttc acgatgtgga tgagggcaag cggatcatgg
     2941 atcaggtggc gtcacgcgga tgatttttcc gttcctacgg cgtgcacagc tcgaacgcca
     3001 accatcccgc gcctgtcaga acaaccacga gagggaggaa ggctgcggcg cccgcgattg
     3061 tccacgttct gacgttgtcg ggtggcagga atcgtgcggc gtacgcacgc caaagggcga
     3121 acgccgaacc agggatcacg acgatacacg aggtgaacaa acctggaacg tagccgcgga
     3181 agcgtatggc gaagaggatc gtgcaaaggg cgacagccaa ccagaatccg ttgcacgcca
     3241 gccacaaacg gattcgtata ccttcgggat cgcggaacgc cgcgaatccg gccgcgcacg
     3301 tgcacagcac cagtaaagcc agtacaactc gaaaggcacc ttccggaatc gagaagtaga
     3361 ggacattcga gaggaaccag gtgtacgcca gcacctgttc gattccccac gtgaatgccc
     3421 cgagggtgc
//
LOCUS       DAOTNG010000322         3414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21866_length_3414_cov_6.471569, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000322 DAOTNG010000000
VERSION     DAOTNG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21866_length_3414_cov_6.471569"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="P5179_12500"
     CDS             <1..407
                     /locus_tag="P5179_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96081.1"
                     /translation="RKRHATAYSLVLDPNPNKRKDELVLAFPAQYVEPELPAVAAARD
                     TVTADSAEPSGEAESTAPMDSAQASVQTPPVAPKPAATVESAPAPPPPAAEDVPAPVA
                     DQPAGTADTTAPVAEPESEQPPAPVESPQNGQ"
     gene            555..1055
                     /locus_tag="P5179_12505"
     CDS             555..1055
                     /locus_tag="P5179_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRS96082.1"
                     /translation="MSAQNSDIPADVAKLLAQFARLSQRFSDQDEIDADMIRLLQQFM
                     GAKRPFHGFSEHVWRPPTDVYETEDSMVVKVEIAGVEKDAFHLEFSRNVLIVRGRRSQ
                     KLGISQVSYHRMEIKYGEFEVEFALPLGLDQDRTTAHYRDGFLTITIPKEDTQITRSV
                     SIDIQG"
     gene            1061..>3414
                     /gene="lon"
                     /locus_tag="P5179_12510"
     CDS             1061..>3414
                     /gene="lon"
                     /locus_tag="P5179_12510"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRS96083.1"
                     /translation="MPDSQDTIPFHTPDSQAPEFPETLGVLMREDLLIFPLMIAQLQI
                     REASTTRLIDEALSTHRIIGVLAAPAPEGDRPKLPELPRIGSAVVIHKMLKLPDGTVS
                     ILVQGVARVRFTEFLQTKPYVLARGTRLESETDDSVETQALARNVTELFQRLIGLVPQ
                     LPDEWKAVVQTMSRNPDHLSDFVAANINITNENRQKLLETLVVRERLSHLSTLLNNEL
                     EVVELSNKIQGQVQSEMSKNQREFFLRKQLETIRQELGEEDEQTAEINELQKKLEEAN
                     LPQQAKEAAAREIDRLRRMPPAAAEYTVARTYLDWLINIPWSVSTEDNLDIRRAKKIL
                     EADHYGLAKIKDRILEYLAVRKLKADMKGPIICFVGPPGVGKTSLGQSIAHALGRKFV
                     RLSLGGIHDEAEIRGHRRTYIGALPGRIVQGLRRAGSNNPVFMLDEVDKIGQDFRGDP
                     SSALLEVLDPEQNSAFSDHYLEVDVDLSKVMFITTANQLAPIPPPLRDRMEVLELPGY
                     TEEEKVQIAKRHLLPRQREAHGLTAEQISFNDAAVRAIIGSYTREAGVRNLEREIANI
                     CRKVARKIAEGEKPPFVVGKKEVEEYLGPARFDSETAERMLDVGVVTGLAATTVGGDI
                     IFVEATKMPGTGKLILTGQLGDVMRESAQAALSYVQSQQKKYHIEDVDFSKTDIHIHV
                     PAGATPKEGPSAGVTLVTALVSLLTGRRARGDVAMTGEITLRGKVMPIGGVKEKTLAA
                     QRAGIHTVILPARNKRDLEDIPKTVRKKVTFVFAEQIDEVLKVAL"
BASE COUNT          746 a          976 c         1070 g          622 t
ORIGIN      
        1 cgcgcaagcg gcacgcgacg gcgtacagcc ttgttctgga cccaaacccg aacaagcgca
       61 aggatgaact ggttctggcg tttccggcgc agtatgtgga gcctgaactg cctgcggtgg
      121 ccgcggctcg tgacacagtg actgccgatt cggcggaacc ttcgggtgaa gcggagagca
      181 ctgcgccgat ggattcggct caggcgagtg ttcagacgcc gcctgttgca ccaaagcccg
      241 cggcaaccgt tgagtcggca cccgcgcctc ctccacctgc cgcagaggac gtaccggcgc
      301 ccgtggcaga tcagcccgcc ggtacggcag acaccacggc gcccgtggcg gaaccggaat
      361 ccgaacagcc tcccgcgccg gtggaaagcc cccagaacgg gcagtaggct gtatcaatcc
      421 ttctttggca cagctattgc gcttgtatac ccatttcagg gattgtccct ttttgcccgg
      481 gacagcctct gctcctgcgc ccgggcccgg gccactgcca tgacccggct gcttttcctg
      541 agaaggagtg tgtcgtgtca gcacagaatt cggatattcc cgcggacgtc gcaaagctgc
      601 tcgctcagtt tgcgcgcctt tcgcagcgtt tttcggacca ggatgagata gacgcggaca
      661 tgatccgcct gctccagcag ttcatgggag cgaaaaggcc attccatggc ttttccgaac
      721 acgtgtggcg tcctccaacc gacgtgtatg aaacggagga ctccatggtc gtgaaggttg
      781 agattgccgg cgtggagaag gatgcgtttc atctggagtt ttcccgcaac gtgctgattg
      841 tgcgtggccg caggagccag aagctcggca tctcccaggt gagttaccac cgaatggaga
      901 tcaaatacgg ggagttcgag gtggaattcg cgcttccgct gggattggat caggaccgca
      961 ccacggcaca ctaccgcgat gggttcctga ccatcacgat cccgaaagag gacacgcaga
     1021 taaccagaag cgtctcgatt gacatacagg ggtaatttgc atgccagatt cacaggacac
     1081 aattccattc catacaccgg acagccaggc acccgagttc ccggaaacac tcggcgttct
     1141 gatgcgggag gatcttctga ttttccccct catgatcgcg cagttgcaga tccgcgaagc
     1201 gtcgacgacc cgcctgattg acgaggcgct ttcgacgcac cgtatcatcg gggtgcttgc
     1261 ggcgccggct ccggaagggg acaggccgaa actcccggaa ctgccgcgca taggatcggc
     1321 agtggttatc cacaagatgc tcaaactgcc cgatggcacg gtaagcattc tggtgcaggg
     1381 agttgcccgc gtccgcttca cggaattcct ccagacgaaa ccgtatgtgc tggcgcgggg
     1441 cacacgtctg gaaagcgaaa ccgacgattc ggtagaaacg caggcgctgg ctcggaatgt
     1501 cacggagctt ttccagcggc ttatcgggct ggtgcctcag cttccggacg agtggaaggc
     1561 cgtagttcag acaatgagcc gcaaccccga ccatttgtcg gatttcgttg ccgccaacat
     1621 caacatcacc aacgagaaca ggcagaagct gctggaaacg ctcgtcgtgc gggaacggct
     1681 gtcccatctc tccacgcttc tcaacaacga gcttgaggtt gtggagctga gcaataagat
     1741 ccagggacag gtccagagcg agatgagcaa gaatcagcgg gagtttttcc tgcgcaagca
     1801 gctcgagacg atccggcagg aactcggaga ggaagacgaa cagaccgccg aaatcaacga
     1861 gctgcagaaa aagctggagg aggcgaacct tccgcagcag gcgaaggagg ccgcggcgcg
     1921 cgaaatcgac cgtctccgca ggatgccgcc cgccgccgcg gaatacaccg ttgcccggac
     1981 gtacctggac tggttgatca acatcccgtg gtctgtatcc acggaggaca atctggatat
     2041 ccgcagggcg aagaagatcc tcgaagccga tcattacggg ctcgcgaaga tcaaggaccg
     2101 catcctcgag tacctcgcgg tgcgaaaact gaaggcggat atgaaggggc caattatctg
     2161 ttttgtcggc cctcccggag tgggtaagac gtcgctcggg cagtcaatcg cgcatgccct
     2221 cggccggaaa ttcgtccgcc tgagccttgg cggtatccac gatgaagcag aaatccgcgg
     2281 gcacagacgg acgtacatcg gtgcgcttcc gggccgcatt gtgcaggggc tccgccgtgc
     2341 gggaagcaac aatccggtgt tcatgcttga tgaagtggac aaaatcgggc aggatttccg
     2401 tggtgatccc tccagtgcac ttctcgaagt gctcgatccc gagcagaact cggccttcag
     2461 cgaccactat ctcgaagtgg acgtggatct ttcaaaagtc atgttcatca cgacggcgaa
     2521 ccagctcgcg cctattccgc cgccactgcg ggatcgtatg gaggtgcttg agcttccggg
     2581 gtatacggag gaggaaaagg ttcagatcgc caaacggcac ctccttcccc ggcagaggga
     2641 ggcgcacggc ctgactgcgg agcagatatc ctttaacgat gcggcggtgc gtgccattat
     2701 cggcagttac acccgggagg ccggggtccg gaatctcgag cgggaaatcg cgaatatctg
     2761 ccgcaaagtg gcgcggaaga tcgcggaggg agagaagccg ccgtttgtcg ttgggaaaaa
     2821 ggaagtggag gagtacctcg ggcctgcccg attcgactct gaaacggcgg aacgaatgct
     2881 ggacgtgggt gtcgttacgg ggctcgcggc caccacggtg ggcggcgaca tcatcttcgt
     2941 cgaggcgacg aagatgcccg gcactggaaa actgatcctg acgggccaac ttggtgatgt
     3001 gatgcgcgaa tcggcacagg cggcgctttc gtacgtgcag tcgcaacaaa agaaatacca
     3061 tattgaggat gtggacttca gcaaaacgga cattcatatc catgtccccg ctggagcgac
     3121 gcccaaggag ggaccgtctg ccggtgtcac gctggtcacg gcgctggttt cgcttctcac
     3181 aggccgccgg gcgcggggtg acgtcgccat gacgggggag atcacactcc ggggaaaagt
     3241 gatgccgatc ggaggcgtga aggagaaaac cctcgcagca cagcgggcgg gtattcacac
     3301 cgtgattctg cccgcgcgca acaagcgtga tctggaggac atcccgaaga cggtgcggaa
     3361 gaaggtgaca ttcgtcttcg cggaacagat tgacgaggtg ctgaaagtcg ccct
//
LOCUS       DAOTNG010000323         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_21983_length_3398_cov_5.152558, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000323 DAOTNG010000000
VERSION     DAOTNG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21983_length_3398_cov_5.152558"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="P5179_12515"
     CDS             complement(<1..806)
                     /locus_tag="P5179_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRS96084.1"
                     /translation="MQRRRTILIIADGTRPDVLGELIAAGELPAIAEAFPEPSMRRSA
                     VSVFPSTTGPAFLPFLTGMYPGDLNMPGIRWFDRGAWAERNGDPPFRSYVGIESYRMN
                     ADFRRDVPTLFDILPRSASIFSSVNRGVPFRGNMTGLSRAWWWLFAHWTDRWDVVGVR
                     ARKRLLASLEMDPEFVFCLIPDVDNYSHLSHCRSERAIAAYRRLDRTIGELFRELERR
                     GWREDTLVVMVADHGHSAVHTHLGLPEWMAGRGFKPFYYPRIWNGTFDSAV"
     gene            complement(852..1268)
                     /locus_tag="P5179_12520"
     CDS             complement(852..1268)
                     /locus_tag="P5179_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96085.1"
                     /translation="MTFRARILFAVVAVFAACVVPHAEPYADSSRVSPCNARTAFALG
                     LVVPGGGQFYLGDRAGGIQAVITDVILFEALRRNDATISGVVFFIGAVHVVEALFATR
                     ECTLRNAAMRPRVTEAFPERREAPAIGLAAAFPISR"
     gene            complement(1419..2543)
                     /locus_tag="P5179_12525"
     CDS             complement(1419..2543)
                     /locus_tag="P5179_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS96086.1"
                     /translation="MRRILEIVRKEFAQLRRDRRLLGILIVAPVFQLFILGYAATTDL
                     REVPVAVCDMDRSASSRSLLDAVSASGDFRLRYFVDAPQELDVYLDRNKADLALVIPR
                     DYGRRVIRGEAATVQVLVDGTRTNAGLALSQLSGAIGQHAQFILLDRLTKRGISGERP
                     EVQLATRVWYNPELKSRNFMLPGVLALVLLIIATAATSMAIVREKENGTIEQIIVTPI
                     RSLELIAGKLIPFAIVGMVEVLLVLSVATLWFRVPLKGSGVLLLTLTFLFVITTQGMG
                     LFVSTVSRTQQQAMMIIIFFIALPMILLSGFVFPIENMPKPIQPITYLMPLRYFLVIV
                     RGIFLKGVGMEVLWPRVVALAAFAFGILALAVARFQKRVG"
     gene            complement(2540..>3398)
                     /locus_tag="P5179_12530"
     CDS             complement(2540..>3398)
                     /locus_tag="P5179_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS96087.1"
                     /translation="GDALAVLVFPPDFGKALRDGRTATAQVLIDGSNANTASIALAYF
                     TAKAREFSAAQLQEFTLARGITQPAMPVVIEPRVWYNAELKSSQLLVPGLIGYLLMLA
                     GTVATSLSVVREKERGTMEQLAVSPLGSGEFILGKTIPYLAVSLLTSGLVIAAAMVLF
                     EMPLRGSIPWLFVTTFVFLLGALGLGLWVSTLVDTQQLALQISLIITLLPALLLSGMV
                     FPIASMPRALQWVSAIVPPRHFLVIMRSILLKGTGPKAWLAELGYLTAFAVFILGIAV
                     VRTRRRSEA"
BASE COUNT          778 a         1185 c          902 g          533 t
ORIGIN      
        1 acggccgaat caaaggtgcc gttccagatg cgcgggtagt aaaacggctt gaatcctcgc
       61 ccggccatcc actccggcag ccccagatgc gtgtgaaccg ccgagtgccc gtggtcggcc
      121 accatcacca ccagcgtatc ctcccgccag cctcgccgct ccagctcccg gaaaagctcc
      181 ccgatcgtcc gatccagccg ccggtacgcc gcaatcgccc gctccgaccg gcagtgcgaa
      241 aggtgcgagt aattgtccac gtccggaatc aggcagaaca cgaattccgg atccatctcc
      301 agcgacgcaa gcagccgctt cctcgcgcgc acgcccacca catcccagcg gtcggtccag
      361 tgcgcgaaca accaccacca cgcccgcgat aggccggtca tgtttccccg gaacggaaca
      421 ccccggttca ccgaactgaa gatgctcgcg gaacgaggca gaatatcaaa aagcgtcggc
      481 acgtcccgac ggaaatccgc gttcatccgg tagctctcga tcccaacgta gctccggaac
      541 ggcggatcac cgttccgctc agcccacgcg ccgcggtcaa accagcggat acccggcatg
      601 ttcaaatcgc ccgggtacat tccggtcagg aacgggagaa acgccggacc cgtcgtggaa
      661 ggaaacacgc tcaccgccga acggcgcatt gacggctcgg ggaacgcctc cgcgatcgcc
      721 ggaagttccc cggccgcgat caactccccg agcacgtccg ggcgcgtccc gtccgcgata
      781 atcaatatcg ttcgtctgcg ttgcattctg ataggttctt tctacagggc acctgcgcgc
      841 cagaaggcag atcagcggga aatcgggaac gccgccgcca gcccgatcgc cggagcttcg
      901 cgccgctccg ggaaggcctc cgtaaccctg ggccgcatag cggcgtttct cagtgtgcac
      961 tcccgcgtgg caaacagcgc ttcaacaacg tgtaccgcgc cgatgaagaa aacaaccccg
     1021 gaaatcgtgg cgtcgttcct ccgaagcgct tcaaacagga tcacatccgt aatgacggcc
     1081 tgaatccccc ccgcgcggtc gccaaggtaa aactgccctc cccccggtac cacaagaccg
     1141 agcgcgaacg cggtccgcgc gttgcacgga gaaacacggc tggaatccgc atacggttcc
     1201 gcatgcggaa ccacgcacgc cgcaaacacc gccacaacgg caaacaggat gcgcgctcga
     1261 aaggtcattc acggctccgg agaggcttca gtccggtcac tcgttcagga aaggaaaaag
     1321 taaaagggaa caatgaagag gcgctataac gacaatgaag attcgttcat gttccaccac
     1381 cgcaccggtc tcaacacccc gaccacgggc cgtccgtcct accccacccg cttctggaat
     1441 cgtgccaccg caagcgccag tatcccgaac gcgaacgccg ccagcgctac cacccgcggc
     1501 cacagcacct ccatccccac gcccttcagg aatatccccc gcacaatgac gaggaaatac
     1561 ctcagcggca tcagatacgt aatcggctgt atcggctttg gcatgttctc aatcgggaac
     1621 acgaaaccgg agagcagaat catcggcagc gcaatgaaaa agatgatgat catcatcgcc
     1681 tgttgctgcg tccgcgaaac cgtggatacg aacagaccca tgccctgagt cgtgatgacg
     1741 aaaaggaacg tcagcgtcag caggagcacc ccgctcccct tcaacggcac ccgaaaccac
     1801 agcgtcgcta ccgaaagcac cagcagaacc tccaccatcc cgacaatcgc gaacgggatc
     1861 aatttccccg cgatcaattc cagagaacgg atcggcgtca cgatgatctg ttcaatcgtc
     1921 ccgttttcct tttcccgcac gatcgccatg gatgtcgccg ccgtcgcaat aatcaggagc
     1981 acaagcgcca gcacccccgg gagcatgaaa ttccggctct tcaactccgg attgtaccag
     2041 acccgggtcg ccagttgaac ctccgggcgc tcgccggaaa taccgcgctt cgtcaggcgg
     2101 tcaagcagta tgaattgcgc gtgctggccg attgcacccg aaagctggct cagcgcaagc
     2161 cccgcgttcg ttcgcgttcc atctaccaga acctgcaccg tcgcggcttc tccccggata
     2221 acccttcgtc cgtagtcccg cggaatgaca agcgcgaggt ccgccttgtt gcggtcgaga
     2281 tacacatcca gttcctgcgg cgcgtccacg aaataccgca ggcggaaatc cccgcttgcg
     2341 ctcaccgcgt ccagaaggct cctgctggac gccgacctgt ccatgtcgca caccgcaacc
     2401 ggaacctccc gcagatccgt cgtcgcggcg tacccgagga tgaaaagctg gaataccggc
     2461 gcaacaatga ggatccccag caaccgccgg tcgcgccgga gttgcgcaaa ttccttccgc
     2521 acgatttcca ggatgcgtct catgcctcgc tccggcgccg ggtgcgaacc accgcgattc
     2581 cgagaatgaa cacggcgaaa gcggtcagat aacccagctc ggcaagccac gccttcggtc
     2641 ccgtcccttt gagcaaaatg cttcgcatga tcaccagaaa atgccgcggc ggcacaatcg
     2701 cggaaaccca ctgaagcgca cggggcatgc tggcgatggg gaacaccatt ccggaaagca
     2761 gcagtgcggg caaaagggtg atgatcaggc tgatctgcaa cgcgagctgc tgcgtgtcta
     2821 caagcgtcga aacccacagc cccagaccca gcgcgcccag cagaaacacg aacgtcgtca
     2881 caaacagcca cgggatgctg ccgcgcaacg gcatctcgaa cagcaccata gccgccgcga
     2941 tgacgagccc gctggtcaga agggatacgg cgagataggg aatcgtcttc cccagaatga
     3001 attcacctga tccgagagga cttaccgcca gctgctccat cgtcccccgt tccttttctc
     3061 gaaccaccga aagggaagtc gccaccgttc ccgcaagcat cagaaggtag cctatcaagc
     3121 caggcaccag aagctgcgag ctcttcagtt ccgcgttgta ccacacgcgt ggctcgataa
     3181 ccaccggcat cgccggttgc gtaattcccc tcgcaagagt gaactcctgc aactgcgcgg
     3241 ccgaaaactc ccgcgccttt gccgtgaagt acgcaagcgc aatacttgcg gtgttcgcgt
     3301 tcgaaccgtc aatcagcacc tgcgcagtcg cggttcgccc gtcccgcagc gctttcccga
     3361 aatccggcgg aaaaaccagc acggccagcg catcgccc
//
LOCUS       DAOTNG010000324         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22046_length_3390_cov_4.773913, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000324 DAOTNG010000000
VERSION     DAOTNG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22046_length_3390_cov_4.773913"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1652)
                     /locus_tag="P5179_12535"
     CDS             complement(162..1652)
                     /locus_tag="P5179_12535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HRS96088.1"
                     /translation="MAASKRIIAIDLGASSGRTILGELGKKLSLREIHRFPNGPVSDN
                     GRMRWDFDRLWQSVQEGIRRASVDAGGDIASLGLDTWGVDYGLLGADGRLLEPPVHYR
                     DSRTNGMMEFAFKIIPRKEIYETTGLQFLQLNTLYQLLAEQMSRSGLLEKAKTLLMIP
                     DVFDYLLTGVAKAEFTDVTTTQCYDPRARGWARSMLERLGIPTHFLPEIVSPGTPLGS
                     VRPELAARLGLQGARVIAPGCHDTASAVAAVPARDGGKWAYLSSGTWSLMGVELPEPH
                     ITETALAYNFTNEGGIGGTFRFLKNISGLWLLQESLRYWREHDGEDISYAEAVARAKA
                     ARPFQCWVDPDAPDFNTPASMPEAISRYCANTGQVVPRTRDEVLRTVFESLALKYRSV
                     FDMLREMVGDIQVLHIVGGGSANELLNQFTANALGVQVVAGPVEATAIGNLMVQAMAL
                     GEVRSTAEIRTVVRDSFAVALFEPHDSADWEDAYGRWREVIAKSVA"
     gene            1943..2737
                     /gene="dapF"
                     /locus_tag="P5179_12540"
     CDS             1943..2737
                     /gene="dapF"
                     /locus_tag="P5179_12540"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRS96089.1"
                     /translation="MVGAGNDFVVIDNRNTSLPEGEPRARFVRDVCQRRLAVGADGVL
                     LLENAENAHFRMRYYNADGGEAEMCGNGGRCIARFAYLHGIAPARMRFVSAAGTHEAE
                     IRGANVKLQMTPPHSLSLNNHVEISGGTRRLHTLNTGVPHAVEVVADVDAVDVVGIGR
                     EIRYHEVFRPAGTNANFITPLDRHTFRIRTYERGVEDETLACGTGTTAAAIIGSFLGL
                     FDSPVVGITQSGLPLTIHFVNRGERAEDVFLEGNADVAFTGSVTAL"
     gene            2796..>3390
                     /locus_tag="P5179_12545"
     CDS             2796..>3390
                     /locus_tag="P5179_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96090.1"
                     /translation="MMTDLRQSLELLSREARAKGYSEEKSTSIQLRIFREFVERERER
                     LHYEHRAGASGHHVVQQWTAVVDAVVIEAYRAVMLNGEGTPGECALIALGGYGRGALN
                     ICSDVDLLFLFAGEVPEQSPIVHGVLHLLWDVGFDLGHSARSLQSALDFASRDDIAQT
                     AVVDARFLAGLEPLFGRFVSSFRARFMADGGWEFSEKV"
BASE COUNT          672 a         1030 c          972 g          716 t
ORIGIN      
        1 cgcctgccag agcgtgtccg tttccctgcc cccccttttg ttctgccagt tattgcggct
       61 tttcggattc cggtcaccgg caccccgcag attcagtgta cgagttttgc agggcgcttt
      121 gaaaatcgtg ggtgtttgcg acgatgtcgg tgcctcccgt actacgcgac ggatttcgcg
      181 attacctccc gccagcgtcc gtacgcgtct tcccagtcgg cagaatcatg tggctcgaac
      241 agcgcaactg cgaatgaatc ccggacgacc gttctgattt ccgccgttga gcgtacttct
      301 cccaacgcca tcgcctgcac catcagattg ccaatcgccg ttgcttccac gggcccggcc
      361 accacctgaa caccgagggc gttggcggtg aactggttca acagctcgtt cgccgatcct
      421 ccaccgacaa tgtgaagcac ctgaatgtcg ccgaccattt ccctcagcat gtcaaacacc
      481 gaccggtatt tgagggcgag gctctcaaat acggtacgca gaacctcgtc cctggtccgg
      541 ggtaccacct gcccggtgtt tgcgcaatat cgagaaatag cctctggcat cgaggccggc
      601 gtgttaaagt ccggcgcatc aggatcaacc cagcactgga atggacgcgc cgctttcgcc
      661 cgcgcgactg cttcggcata tgagatatcc tcaccatcat gttcccgcca gtaccggaga
      721 gattcctgca gcaaccagag gccgctgatg tttttcagga accggaacgt gcccccgata
      781 ccgccttcgt tcgtgaagtt gtacgcaagc gcggtctccg taatgtgcgg ctctggcagt
      841 tccacgccca tcagcgacca cgttcccgag ctcagatacg cccacttccc gccgtcacga
      901 gcgggaactg ccgccaccgc ggacgccgta tcgtgacatc cgggagcgat gactctggcg
      961 ccctgcaacc caagccgggc agccagctcc ggacggacag agcccagcgg tgtacccggg
     1021 gaaacgatct ccggcaagaa gtgcgtcggt attccaaggc gttccagcat ggagcgggcc
     1081 cagccgcggg ctcgagggtc gtaacactga gtagtagtga cgtccgtgaa ttccgccttt
     1141 gcgacccccg tcaggaggta atcaaaaacg tcggggatca tcagcagcgt tttcgccttt
     1201 tcgagaagcc ccgagcgaga catctgttcc gcgagcagtt gataaagcgt gttcagttgg
     1261 aggaactgca gacccgtcgt ctcgtatatc tccttgcgag gaattatttt gaacgcgaat
     1321 tccatcatcc cgttcgtgcg cgaatcccga tagtggaccg gcggttccag caatcgcccg
     1381 tccgcgccca aaagaccgta gtccacgccc cacgtatcca ggccaaggct tgcgatgtct
     1441 ccgccggcat ccacggaagc ccgccggatg ccctcctgca cggattgcca gagacggtcg
     1501 aaatcccagc gcatgcggcc gttatcggaa acaggcccgt tcggaaaccg gtgaatctcc
     1561 ctcaggctga gtttctttcc cagttcaccc agaatcgtgc gtccgcttga tgcgcccaga
     1621 tcaatggcga ttattctctt cgacgccgcc atctcgatta tctcctgcca agattacaaa
     1681 agcctgtaca tccgggcccg gaatcccttt tcaaggccca gatgagcgct gactcccgca
     1741 gtgttgaaaa tgcaacagtt gccgcgtttt gcacaagcgg cggacgcccg atgtcccctt
     1801 gacgtcaaag gtctcggagg gcgatttttt tgatagtctc aagcactttc catgtcccgg
     1861 aaagccgttc tctcacgacc gccaatctgc cacctgccgc aacgtattcg aggacaccgc
     1921 attggatatc ctgtttacaa aaatggtcgg agcgggtaac gattttgtcg taatcgacaa
     1981 ccggaacacc tccctgcctg agggtgaacc gagagcgcga ttcgttcgcg acgtgtgcca
     2041 gcgcaggctt gccgttggag ccgacggtgt cctgcttctg gaaaatgccg agaacgcgca
     2101 tttccggatg cgatattata acgctgatgg cggggaagcg gaaatgtgcg gcaatggcgg
     2161 tcgttgtatc gcccgctttg cgtacctcca cggaattgcc cctgcccgga tgcgtttcgt
     2221 atcagcggca ggaacccacg aggcggagat ccggggtgcg aacgtgaagc tccagatgac
     2281 cccgccgcat tcgctttcgc tgaacaacca cgtcgagatc tccggaggaa cgcgcagact
     2341 gcacacgctt aacaccggag tccctcacgc ggtggaggtc gtggctgatg ttgacgccgt
     2401 ggatgtagtc ggcatcgggc gcgagatacg gtaccacgaa gtgttccgcc ctgcgggcac
     2461 aaacgccaac ttcatcacgc ctctggacag gcacacgttc cgcatcagaa cgtacgaaag
     2521 gggagtggaa gacgaaacgc ttgcctgcgg aaccggcaca actgccgcgg ctatcatcgg
     2581 atcgttcctc gggcttttcg attctccggt tgtcggtatc actcaaagcg gtttgccttt
     2641 gaccattcac ttcgtgaatc gaggagaacg cgcggaagac gtgttcctcg agggcaacgc
     2701 cgacgttgca ttcaccggct cagtaacagc attgtaacgt tcatgttaca caatcacccc
     2761 actcggaaca aaactcctta acgggcgtcc cgccgatgat gaccgatttg cgtcagagcc
     2821 tcgaactgct ttcccgggaa gcgcgcgcaa aaggttattc ggaagagaaa agcacctcga
     2881 ttcagttgcg gattttccgg gagttcgtcg agcgggaacg tgagcggttg cactacgagc
     2941 accgggcagg cgcttcggga catcatgtgg tgcaacaatg gaccgccgtg gtggacgcag
     3001 tcgttatcga ggcataccgt gcggtgatgc tcaacgggga gggaactcca ggcgagtgtg
     3061 cgttgattgc tctcggcggg tacgggagag gcgccctcaa catctgctcg gatgttgacc
     3121 tgttgttcct gttcgcgggc gaggtacccg agcagtctcc gattgtgcac ggggttcttc
     3181 acctgctctg ggacgtcggg tttgatctgg gccattccgc ccgaagtttg caaagcgctc
     3241 tcgactttgc ttccagggat gacatcgcgc agaccgccgt cgtggacgcg cgttttctcg
     3301 ccgggctcga gcccctgttc ggtcggtttg tgagcagttt ccgcgcgcgt ttcatggcgg
     3361 acggcgggtg ggagttttcg gagaaagtcg
//
LOCUS       DAOTNG010000325         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22062_length_3388_cov_5.250825, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000325 DAOTNG010000000
VERSION     DAOTNG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22062_length_3388_cov_5.250825"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="P5179_12550"
     CDS             <1..481
                     /locus_tag="P5179_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96091.1"
                     /translation="DRPDTYAFRSTLIPGATLTTTVARPDEPEGYRTPWDAFPVEWLQ
                     TMAKQYQELKPYALGDFYPLISFSLADDVWAVWQFHRADLESGVILAFRRQNSPYPEM
                     RVPLKGVLPNARYRIQSYDGPGARIWRSGDLTAEGFSIIIGERPGSRLFRYELAEKA"
     gene            complement(556..1488)
                     /locus_tag="P5179_12555"
     CDS             complement(556..1488)
                     /locus_tag="P5179_12555"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRS96092.1"
                     /translation="MKNFKPAYGIVPPLITPFNEDLSIDWYAYDRLIDWHIERNVTGL
                     FVVCGSSEYWKLTEDEAIQMAKAAVKRANGRIHILAGSTNYPDDDLTKNINMTKRMAD
                     TGVDGCFITTPRTIPPQDDLMLDYHMKIHDAVNYPLYAYEMPGGTNYKFSPEAFAALG
                     ATERFIGIKDTTCDLAKVAKKIAAAKGTIKIMQAHTPTLLDSCKLGATGGINTSSNVC
                     PSLFQKLFDLFDAGDLETAERLQQRIIQIDEMMSDGYVKSAKVAVSMMGVPMKHFTRN
                     PSKDFTPERMEELKKMCELIEESEKEFGIVHRSI"
     gene            complement(1653..>2214)
                     /locus_tag="P5179_12560"
     CDS             complement(1653..>2214)
                     /locus_tag="P5179_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS96093.1"
                     /translation="RMGEKSATNLVAAIQASKSRPFPAFVYALGIRNVGETTARALCR
                     RFRSIDTMMSASVESFMEVEDVGPVVAESIVDFFRVEENRRLVERLKAHGLQTESKEN
                     QASEAPPPGKFTGMTFVLTGALERFTRDDAAERIRAKGGTVTDSVSRKTSIVVAGPGA
                     GSKLAKATQLGIEIWDEARFLAELGE"
     assembly_gap    2215..2314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2315..>3388)
                     /gene="ligA"
                     /locus_tag="P5179_12565"
     CDS             complement(<2315..>3388)
                     /gene="ligA"
                     /locus_tag="P5179_12565"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRS96094.1"
                     /translation="GDDITDNARTIRTIPLRLRHGLSDVEVRGEVYLPTAEFRRMNEA
                     RIEAGEKAFANPRNAAAGTLKLMDPKEVARRPLAFVAYWLDDRHNDGGTQWHALELLQ
                     NLGFVVSEHRRLCRSFSEVLLFCEEWEEKRDALPYEIDGVVVKVNRLDFQEQLGYTSK
                     SPRFMIAYKFRARKAETTLLEIGLQVGRTGAVTPVAILEPVLVGGITISRATLHNADE
                     IRRKDIRPGDTVIVERGGDVIPKVSGVVLEKRPPTSVPFEFPTACPVCGAILFRGAED
                     AITRCENAACPAQVRGRILHFASRPAMDIEGLGPAVVDQLVNAGLVHNAADLYELEPG
                     RVAALERMGEKSATNLVAAIQASK"
BASE COUNT          655 a         1023 c          869 g          741 t
ORIGIN      
        1 cgacaggcct gacacgtatg cattccggag cacgctcatt ccgggggcca cactcactac
       61 caccgtcgcc cggcccgatg aacctgaagg gtatcggaca ccctgggatg cgtttcccgt
      121 agagtggctc caaaccatgg cgaaacagta tcaggagctg aagccctacg cgctcggaga
      181 tttttacccg ctcatctctt tcagcctcgc ggacgatgtg tgggcggtat ggcagttcca
      241 tcgtgcggac ctcgaatcag gcgtcatcct tgcattccgt cgccagaaca gcccgtatcc
      301 ggaaatgcgt gttcccctga aaggagtcct cccgaacgcc cggtatcgta tccagtcgta
      361 cgacgggccg ggcgcacgaa tctggagaag cggcgatctc accgccgagg gattctccat
      421 cattatcggc gaaagacctg gctcccgcct gtttcgttac gagctcgccg aaaaggcgtg
      481 atgactgcaa aaaaagaagc gggagactcg ttcaacgagt ctcccgcttc tgattggtcc
      541 cgatcgaacg cgttttcaga tcgatcggtg aacgatccca aactccttct cactttcctc
      601 gatcagttcg cacatctttt tcagctcttc catgcgctcc ggtgtgaagt ccttggaagg
      661 attgcgggtg aaatgcttca tgggaacgcc catcatactg accgcgacct tggccgattt
      721 cacgtaccca tcggacatca tttcgtctat ctggatgatc cgctgctgga ggcgttctgc
      781 cgtttcgagg tcccccgcat cgaacagatc aaaaagcttc tggaaaagac tggggcacac
      841 atttgatgag gtgttgatgc ccccggtcgc gccgagtttg caggaatcca gcaacgtcgg
      901 ggtgtgtgcc tgcatgatct tgatcgtccc cttcgccgcg gcaatcttct tcgcaacttt
      961 cgcgaggtcg cacgtggtgt ccttgatccc tatgaatctt tcggtcgctc cgagcgccgc
     1021 gaacgcttcc ggagagaact tgtaattggt cccgcccggc atctcatacg cgtacagcgg
     1081 ataattgacg gcatcgtgga tcttcatgtg ataatccaac atcaagtcgt cctggggcgg
     1141 gatcgtgcgg ggtgtagtaa tgaaacagcc gtccacgccc gtgtctgcca tccgcttcgt
     1201 catgttgatg ttcttggtga ggtcgtcgtc cgggtagttg gtactcccgg ccaggatatg
     1261 aatcctcccg ttcgctcgtt tcaccgcggc cttcgccatc tgaatggctt cgtcctcggt
     1321 cagcttccag tattcgctcg acccgcacac gacaaaaaga ccggtgacat tccgctcaat
     1381 gtgccagtca atcagtctgt cgtaggcgta ccagtcaatc gacaagtcct cgttgaacgg
     1441 cgtaataaga ggcggaacaa tcccgtacgc cggtttgaaa ttcttcactg cgaaatcctc
     1501 ccgcggcagg ggcaaggaac cagattaccg ttcaggaaaa tgcatccacc cgccggtgcc
     1561 gcacggccgt caggtgattt ccatgtatga aagatgaagc gcattcaaga atgggtcaac
     1621 caagggcccc acggttgttc gaacaagggg cgtcattccc ccagttccgc cagaaaacgc
     1681 gcttcgtccc agatctcgat tccaagttgc gttgctttcg ccagcttgga gcccgcaccg
     1741 ggtcccgcca caacaatgga ggtttttcgg ctcactgagt ccgtcactgt tcctcctttt
     1801 gcacggatac gttccgccgc atcgtcgcgc gtgaatcgtt ccagcgcacc cgtaagaaca
     1861 aacgtcatcc ccgtgaactt tcccggtggc ggtgcctccg atgcctgatt ctccttggat
     1921 tccgtctgca gaccatgggc tttcaaccgt tctacaagtc tgcggttctc ctctacgcgg
     1981 aagaagtcca cgatactctc tgccacaacg ggtccgacat cctctacttc catgaacgat
     2041 tccaccgaag cgctcatcat tgtatcgatg gaacggaaac gccggcagag cgcccgtgcc
     2101 gtggtttcac ccacatttcg gataccgaga gcatagacaa acgcgggaaa cggtcgcgat
     2161 tttgaagcct gaatcgcggc aacgaggttc gtggccgatt tctctcccat gcgcnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttga agcctgaatc gcggcaacga
     2341 ggttcgtggc cgatttctct cccatgcgct ccagtgccgc tacccgcccg ggttcaagct
     2401 catacaggtc tgcggcgttg tgaaccaggc ccgcattcac cagctgatcc accaccgccg
     2461 gccccagccc ctcgatgtcc atcgccggcc ggctggcgaa atggagaatc cttccgcgaa
     2521 cctgcgccgg gcacgccgca ttctcacatc gcgtgatcgc atcctccgct ccacgaaaca
     2581 gaattgcgcc acacaccggg cacgcggtcg gaaattcaaa cggaaccgat gtaggtggcc
     2641 gtttttcgag gaccacgccg ctgactttcg ggatcacgtc cccgcctcgt tccacgatta
     2701 cggtgtcgcc gggccggata tccttgcgcc gtatttcgtc ggcattgtgc agtgtcgccc
     2761 ggctgatcgt tatcccgccg acgagaaccg gttccagaat cgccacgggt gtcaccgctc
     2821 cggttcggcc aacctgcagt ccgatttcga ggagagtcgt ttcggctttt cgcgcccgaa
     2881 atttgtacgc gatcatgaag cgcggactct tggacgtgta gccaagctgt tcctgaaaat
     2941 ccagccggtt caccttgacc acgacgccgt ctatttcgta gggcagggcg tcgcgtttct
     3001 cctcccattc ttcgcagaac agaagcactt cggagaacga cctgcacagc cgccgatgct
     3061 cacttacaac gaaacccaga ttctgaagta attcgagcgc gtgccactgc gtgccaccat
     3121 cattgtgccg gtcatccagc cagtacgcaa caaaggcgag cggacggcgt gccacttcct
     3181 tcgggtccat aagtttgaga gttcctgcgg cagcgttacg cggattcgcg aaggcttttt
     3241 cgccggcctc gatgcgagct tcattcatcc ttcgaaattc agccgtcggc agatacactt
     3301 ctccacgcac ttccacatcg ctcagcccgt gccgcaggcg aagaggtatg gtgcggatgg
     3361 ttctggcgtt gtccgtaata tcgtctcc
//
LOCUS       DAOTNG010000326         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22096_length_3384_cov_5.042055, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000326 DAOTNG010000000
VERSION     DAOTNG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22096_length_3384_cov_5.042055"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="P5179_12570"
     CDS             complement(<1..516)
                     /locus_tag="P5179_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96095.1"
                     /translation="MKDDCLIGHWKLELDPADASGNNHHGNNTGARFDGSACWFDGVK
                     GHIEIPSLSSSCVGSGDFTISTWVWTAKGQQDITGDIASSFDPHLRKGFNLSIDDRPG
                     LTSSQSNMRNLFFGIDDGLSEPSWRDCGRPGNAILVFAMCVWQGSLYVGTFEGGANER
                     GHVYRYAGESAW"
     gene            complement(513..1325)
                     /locus_tag="P5179_12575"
     CDS             complement(513..1325)
                     /locus_tag="P5179_12575"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96096.1"
                     /translation="MSSAESLIDIHVHLLPGVDDGPRDIDSSVALIEQGLEDGVGCWV
                     TTPHVLGPFDDRVDRLHQSVFQDLVAEVARRRLPAELHLASEIMFGAAADDIKNHRTG
                     TFGGNGRYFLLEYPMMQFPTAAEEALFAAQLAGLRPIVAHPERNADLARRPDLRARLA
                     HRGILFQINARSLSREARPEDRSAAEHMIRNGLAHFVASDGHDPVQRPAFLRFAYERV
                     AELTSEATARRLFAENPRAAIEGRAIQAIREEDTLPMRWWHRLVRRKTEISR"
     gene            complement(1341..2093)
                     /locus_tag="P5179_12580"
     CDS             complement(1341..2093)
                     /locus_tag="P5179_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRS96097.1"
                     /translation="MNAFDRIMATRKTRGAGFWVLIDPDRTAPVEAAARAADAERAGA
                     DVILVGSSLLISDTFGDAVREIRGAVKLPVIIFPGSTAQISADADAILFLSLVSGRNP
                     DLLIGQHVRAAPAIKAIGLEPIPTAYMLVESGTLTTIEYISDTKPIPRQKPDIAMAHA
                     LAAEYMGMRLVYLDGGSGAKEPVPTEMIRACYEYITLPLIVGGGIRSGEAAQAAVKAG
                     ASFVVVGNALEKTSGRSLLTELADAVHSAAPA"
     gene            complement(2236..>3384)
                     /locus_tag="P5179_12585"
     CDS             complement(2236..>3384)
                     /locus_tag="P5179_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96098.1"
                     /translation="GGGTVAGKDVAYGQTPPDGVPKSLAYLYDVFRGQIEEPWSAHWL
                     LDAADGLQLRVTQLTTGSGELIKARGRSPHAPVENPPYELDWLLSHNQRSESDVLRSD
                     FVSLIETGTALPIEDARLIRSGSVSGVRVGFDGGAHYILRSEDGSSVAAVDDDISFQG
                     VAGFVEVDAGGVSRMALIGDGVLTCGDRGITRGAGRWRGSIVAVCPAERAVTVQVSES
                     TLSPGKGDYLYVRRRTGERIDSFAYRITNVLEANGKLVRVWLNWSPVIGDGVVKEHTE
                     TGFRIQGQMPVSAARAYYRGAYLVNHDRTLCTRLKNIRGGWSTQEPVAHLERVDSSTL
                     RAFPPGSAFTVEEIGVGDVVEVLGWVQCERQPDTTWRVESNGPVVTRF"
BASE COUNT          691 a         1099 c          926 g          668 t
ORIGIN      
        1 ccaggcagat tcgccggcat aacggtacac gtggccccgc tcgttggccc ctccttcgaa
       61 tgtccccaca tacaggcttc cctgccagac gcacatcgcg aacacaagga tggcgttccc
      121 cggtcgtccg caatcgcgcc acgaaggctc ggatagtccg tcatcgattc cgaagaagag
      181 attccgcatg ttggattggg aagacgtgag gccgggtcgg tcatcgatac tgaggttgaa
      241 tcctttcctg agatggggat caaacgagga ggcgatatcg ccggtaatat cctgctgtcc
      301 tttcgccgtc cacacccagg tcgaaatcgt gaagtccccg cttccaacac aggaggacga
      361 caacgacggg atctcaatgt gacccttcac tccgtcaaac cagcaggctg acccgtcgaa
      421 gcgagcgccg gtgttgttcc cgtggtggtt gttaccactc gcatcggccg ggtccagttc
      481 cagtttccag tgcccgatca ggcagtcatc cttcaccttg agatctccgt tttccgcctc
      541 accaatctgt gccaccagcg catcgggaga gtgtcctcct cccggattgc ctgaatggct
      601 ctaccctcta tcgctgcccg cggattctct gcgaaaagcc tccgggcggt agcttcgctc
      661 gtaagttccg cgacacgctc gtacgcaaag cgcagaaacg cgggccgctg caccggatcg
      721 tgcccatccg acgcgacaaa atgcgccaga ccattacgaa tcatgtgctc cgcggcgctt
      781 cgatcctctg gccgggcttc tcgtgaaagg cttcttgcat tgatctggaa cagaatcccg
      841 cggtgagcga gccgcgcccg aagatcaggc cgccgggcaa gatcggcgtt gcgctcagga
      901 tgcgcgacaa tcgggcgcaa gccggcaagc tgcgcggcga aaagcgcttc ttcagcagcg
      961 gtggggaact gcatcatcgg atattccagc aggaaatacc gcccgtttcc accgaaggtg
     1021 ccagtcctgt ggttcttgat gtcgtcggcc gcggcgccaa acatgatctc ggacgccaga
     1081 tgaagctcgg caggcaatct tcggcgggca acttcggcga caagatcctg gaaaaccgat
     1141 tgatgcagcc tgtcaacgcg gtcgtcaaag gggcccagaa cgtgcggagt tgtgacccag
     1201 catccgaccc cgtcttccag accctgttca atcagagcca ccgaggagtc aatgtctcgc
     1261 ggcccgtcgt ctacacccgg gaggaggtgg acatggatat ctatcagact ttcagcggag
     1321 gacatcagaa gtgccgattt tcacgcggga gcggccgaat ggacagcgtc agcgagttcc
     1381 gtcaggaggg accggccact tgttttttcg agagcgttgc ccacaaccac gaaggatgcg
     1441 cccgccttta cggctgcctg cgcggcttcg ccggagcgaa ttccacctcc cacgatcaac
     1501 ggcaacgtga tgtattcgta acacgccctg atcatctccg tcgggaccgg ctccttcgct
     1561 ccacttcccc cgtccagata caccagtctc atccccatgt attccgccgc aagagcatgt
     1621 gccattgcga tatcgggctt ttgccgcgga atcggcttcg tgtcgctgat gtattcgatc
     1681 gtggtcaatg tgcctgattc caccagcatg tacgcggtgg gaatgggctc aagaccaatc
     1741 gccttgatcg cgggggctgc ccgcacgtgt tgtcctatca gaaggtcagg attgcgcccg
     1801 ctgacgagcg aaaggaacaa tattgcgtcc gcgtccgctg aaatctgggc cgtactcccg
     1861 ggaaaaatga tcaccggcaa cttgaccgcg ccgcggatct ctcgcaccgc gtcaccgaat
     1921 gtgtccgaaa tcagcaggct gctccccacg agtatgacat ccgcacccgc ccgttccgcg
     1981 tcggctgcgc gcgcagccgc ttcgacaggt gcggtgcggt ccgggtcaat aagaacccag
     2041 aaaccggcac cgcgggtctt ccgcgtcgcc attatccgat caaaagcatt caccagcatg
     2101 ttcccaggaa gaatcagcgt acacgtgcaa acgcccctca ggagtcgtta tccttccgcg
     2161 taagtcttcg tcaaatatac aaaagctccg gttgtctggt aacgtggtcg aggaaacctt
     2221 tccgaagaac ggccactaga atcgtgtgac caccggtcca ttgctttcca cgcgccacgt
     2281 ggtatcgggt tggcgctcac attgcaccca acccagcact tccaccacgt ctcccacccc
     2341 gatctcttcc acggtaaagg ccgatccagg cggaaatgca cgtagtgtgg acgaatcaac
     2401 tctttccaga tgcgcgaccg gttcctgagt cgaccacccg ccgcggatgt tcttcaatcg
     2461 cgtgcacagg gtgcggtcgt ggttgacaag ataagccccc cggtagtacg cccgcgccgc
     2521 ggaaacaggc atctgcccct gaatacgaaa gccggtctcc gtgtgctcct tcacgactcc
     2581 gtccccgatg acgggcgacc agttcagcca cactctgaca agcttgccgt tcgcttcgag
     2641 cacgtttgtt attcggtacg cgaatgagtc gattctttcg cccgttcgcc ttcgcacgta
     2701 gagatagtcc cccttgccgg gggacaacgt gctttcggac acctggaccg ttaccgcgcg
     2761 ctcggccggg caaacagcaa cgatcgatcc gcgccaccgg cctgcccccc tggtgatgcc
     2821 gcggtcgccg caggtcaaaa ccccatcgcc gatcaatgcc attcgggaaa caccgccggc
     2881 gtctacctcg acgaatcccg caacgccctg aaaagagata tcgtcgtcca ccgcggccac
     2941 agaggaaccg tcttccgaac gaagtatgta gtgcgcgcca ccgtcaaacc cgacccgtac
     3001 gccgctcacg gaccccgaac ggatcagtct ggcgtcctcg atgggaagag cagttcccgt
     3061 ttcaatcagg gatacaaaat cggaccgcag tacatcactt tcgcttcgct gattatgact
     3121 tagcaaccaa tccagttcgt acggcgggtt ctccaccgga gcatgcggcg atcttccacg
     3181 ggccttgatc agttcacccg atcctgttgt aagctgagtc acgcgaagtt gtaacccgtc
     3241 cgccgcgtct agcagccagt gcgcagacca gggttcctcg atctgcccgc ggaacacgtc
     3301 gtagagatac gcaaggcttt tcggaacgcc gtcgggaggg gtctgcccgt aagcgacatc
     3361 cttgccggca accgttcccc ctcc
//
LOCUS       DAOTNG010000327         3379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22146_length_3379_cov_3.755415, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000327 DAOTNG010000000
VERSION     DAOTNG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22146_length_3379_cov_3.755415"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="P5179_12590"
     CDS             complement(<1..382)
                     /locus_tag="P5179_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96099.1"
                     /translation="MRTARIRPDLLGPECPEGLEIIQITTGELPSTHIYMEAQIFTPD
                     SKRFLLHESATPHGSDPKDPNHRYLLCDLEQGCELSPVTQETGATAPSVSPDGMHVYY
                     FVDETRLDGGKSTLKRMNLDGSARE"
     gene            complement(419..1189)
                     /locus_tag="P5179_12595"
     CDS             complement(419..1189)
                     /locus_tag="P5179_12595"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRS96100.1"
                     /translation="MIAKWTKTLRNNDTFPRVCAHRGLSKACPENTLPSFGAAIGVGA
                     HEIEFDVRLSRDDVAVVCHDERVDRTADAPGAISEMEWAELRKLDFGARYGEAWRGVR
                     VPRIEEVLDLAGGRVGINVHIKEPGNDSRLVRLVANEIRRRQIVESAYLAGATEAVLS
                     ACVEFAPEIPRACLVSQNGPREQIRVAIKYGCARIQFRRVATQEHFREAHDAGLVCNL
                     FYSDDFEEAFEFGRHGVDVILTNCAHQLIHARDNSHAA"
     gene            complement(1158..1538)
                     /locus_tag="P5179_12600"
     CDS             complement(1158..1538)
                     /locus_tag="P5179_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96101.1"
                     /translation="MVTITTDGVTVRIKVRGLHQLWAFRRTLEIPLRHVTSVTRMDPG
                     LRPPWLRLIGTAIPGLIYAGMFRGRGRREFWDWTCGKDAVQLDLAEETLSRIVVSVDN
                     AEEVIRQISGEFRAGDSKMDENIA"
     gene            complement(1596..2939)
                     /locus_tag="P5179_12605"
     CDS             complement(1596..2939)
                     /locus_tag="P5179_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96102.1"
                     /translation="MGEIWTLLFVDDKHIEATRRVCRVFEQPRKFGPVVVPDRPWEAQ
                     CVIVYGSVLPNPAGSGFQMWYQSYDRSKSGPDRAHNCYSVSEDGVHWEKPELGVHEFR
                     GSRRNNIFAKGLGSLNVIVDRGDPNGEHRYKMLFTGANSEGPGLFAGFSPDGIHWKVG
                     TERVVDSVSDRTTLLFDPELKAPFVGFTRRHGKDMQAEFGRRVIYRIESEDFAHWSEP
                     TPLLVPDLADSWDLQFYGMPVFRYRDLYLGGLKRLWSTPDRIDTELVWSRDTKSWGRS
                     RVPFLDNGPSDSWDSSWVGLASSPPLEIGGQLFFYFEGRRQAHGQRFPFPQGSIGLAV
                     LPKDRFAAIEAGPAEGYVETKPFKWHGGRLGVNLRTNGHGYLRVALLDEDGSEIPGFS
                     APVCTSLTGDIPCAEPSWGNQPPRELIGRSVRLRIHMVNTRLYAVYALAAPNDHA"
     gene            complement(3012..>3379)
                     /locus_tag="P5179_12610"
     CDS             complement(3012..>3379)
                     /locus_tag="P5179_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HRS96103.1"
                     /translation="LWASSTLPGGNSLMQGIVTMPKLVSGSITAFGLYGIVLLFKAPL
                     SLFYGMIGGLTGFPTGTYLLFAGAMLNRFYFYKKFGQATWAKYAPILLAGYGCGMGLV
                     AMSSIAVSLIVKSVSQVIF"
BASE COUNT          713 a         1067 c          895 g          704 t
ORIGIN      
        1 cttcccgcgc acttccgtcg agattcattc gtttcagcgt cgatttccca ccgtcgaggc
       61 gggtttcgtc gacgaagtag tacacgtgca tgccgtctgg cgatactgaa ggcgctgtcg
      121 cccccgtttc ctgtgttacc ggcgacagct cgcaaccttg ttcaagatcg cagaggaggt
      181 accggtggtt cggatccttc gggtcactgc catgtggcgt cgcagattcg tgaaggagaa
      241 agcgttttga atcaggagta aatatctgcg cctccatgta gatgtgggtg gagggcagct
      301 caccggtggt gatctggatg atttccaacc cttccgggca ttcggggccg agcaggtccg
      361 gcctgatcct ggcagttctc acgtgatccc cattccttgc gtgtagacgg gatttggttc
      421 acgccgcgtg gctattatcg cgagcgtgta tcaactggtg agcgcagttc gtcaggataa
      481 cgtcaacacc gtggcgtccg aactcgaacg cctcctcaaa gtcatccgaa tagaacaggt
      541 tgcagaccaa accggcatca tgtgcttcac ggaaatgttc ctgtgtcgcc acgcgacgga
      601 actgaattcg cgcacaaccg tacttgatcg ccacgcggat ctgctcccga ggcccattct
      661 gtgaaacaag acacgcacgt ggaatttccg gggcaaactc cacgcacgca gagagcacgg
      721 cttcggtcgc tccagccaga tacgcggact caacgatctg gcgtctgcga atctcatttg
      781 caacaaggcg gaccaggcgt gaatcgttcc ccggttcttt gatatggacg ttgataccga
      841 cccgtccacc ggcaaggtcg agaacctcct caatccgggg cacacgaact ccccgccacg
      901 cttcaccgta ccgggcaccg aaatcgagct tgcgcagttc agcccattcc atttcggata
      961 tggctcccgg cgcgtccgcg gtgcggtcca ctcgctcgtc gtggcagacc acggctacat
     1021 cgtcgcggct caaccgaacg tcaaattcga tttcgtgcgc accgacgccg atcgctgcgc
     1081 caaatgaagg gagagtgttc tccggacacg ccttgctcag tccacggtgc gcgcagacac
     1141 gcggaaaagt atcattatta cgcaatgttt tcgtccattt tgctatcacc tgcgcggaat
     1201 tctccgctta tctgccgtat cacctcctct gcgttgtcta cgctcaccac tattcggctg
     1261 agcgtttcct ccgcgaggtc cagttgcacc gcatccttgc cacaggtcca atcccagaac
     1321 tccctcctgc cacgcccgcg aaacattccc gcataaatga gcccgggtat agcggttcct
     1381 atgaggcgta accagggtgg gcggagccca ggatccatcc gcgttacaga tgtcacatgc
     1441 ctcaacggta tttccagtgt ccgcctgaaa gcccagagtt ggtggagccc ccggactttg
     1501 atcctgacag tgactccatc tgttgtgatg gtaaccatgg cgtctgtcct cttccggaac
     1561 tcaatcctcg gccatcaccg accgctctct gttcctcacg cgtgatcgtt gggtgcggca
     1621 agcgcgtata ctgcgtacag acgagtgttc accatgtgga ttcggagacg aacgctacgg
     1681 ccgatgagtt cccgaggcgg ttggttcccc cacgatggct cggcgcaggg aatgtccccg
     1741 gtaaggcttg tgcagacagg ggcggagaat cccggtatct cactgccgtc ctcgtccagt
     1801 aacgcgacac gcaggtaccc gtgcccattg gtgcgcaggt tgacccccaa tctgcctccg
     1861 tgccatttga acggtttcgt ctccacgtat ccttccgccg gaccggcttc aattgctgcg
     1921 aatctatctt tcgggagcac cgcgaggcct atcgaaccct gtgggaacgg aaacctttgc
     1981 ccgtgcgcct gacgcctgcc ttcgaagtag aagaaaagct gaccaccgat ttccaacggc
     2041 ggagacgagg ccagacctac ccacgaggaa tcccaggagt cacttggccc gttatccagg
     2101 aaaggcacac gggatcttcc ccacgatttg gtgtctctgc tccacacaag ttccgtgtca
     2161 attcgatccg gtgtcgacca cagacgtttg aggccgccga ggtacaggtc acggtaccga
     2221 aataccggca tcccgtagaa ctggaggtcc cacgaatccg caaggtcggg aaccagcaaa
     2281 ggagttggtt ccgaccagtg tgcaaaatcc tcactttcaa tccggtaaat gacgcggcga
     2341 ccgaattcag cctgcatgtc ctttccatgg cggcgcgtga agcccacgaa gggagccttc
     2401 agttccgggt caaaaagcaa agtcgtcctg tcactcaccg aatcaactac gcgttccgtg
     2461 cctaccttcc agtgaatccc gtccggcgaa aaccccgcaa agagacccgg accttcggag
     2521 ttagcgcccg tgaacaacat cttgtacctg tgttcgccgt tcgggtctcc acggtcaacg
     2581 atcacattca atgaccccaa gcctttggca aagatgttgt tccggcggga cccccggaac
     2641 tcgtggacgc ccaattcagg cttttcccag tgaacgccat cttcgctgac cgaatagcag
     2701 ttgtgcgccc ggtcggggcc actcttggac cggtcatagc tctgatacca catctgaaaa
     2761 ccagaccccg ccgggtttgg aagcacggaa ccgtacacga tcacgcactg cgcttcccac
     2821 ggccgatccg gaaccacaac cgggccgaat ttccgcggct gttcaaacac acggcaaacg
     2881 cgtcttgtcg cttcaatatg cttatcgtcg acgaagagca gagtccagat ttctcccatc
     2941 tcacgccgcc tcaatgaaat gggcgcccgc gaaaggcgcc cgtttgttca gcttgccccg
     3001 gcacacgtgc ctcagaagat aacctgagac acgcttttca caatcagcga cactgcaatg
     3061 gaggacatgg cgaccagacc catgccgcat ccgtagcccg caagcaggat cggcgcgtat
     3121 tttgcccacg ttgcctgccc gaacttcttg tagaagtaga accggttcaa cattgcccct
     3181 gcgaagagca aataggtgcc ggtggggaac ccggtcaggc caccgatcat accgtagaac
     3241 agactcagcg gcgccttgaa cagcagaacg attccataca gaccgaacgc cgtgatcgac
     3301 cccgatacca gtttcggcat cgtcacgatc ccctgcatca ggctgttccc ccccggcaac
     3361 gtgctggatg cccacagcg
//
LOCUS       DAOTNG010000328         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22227_length_3369_cov_4.748340, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000328 DAOTNG010000000
VERSION     DAOTNG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22227_length_3369_cov_4.748340"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="P5179_12615"
     CDS             <1..674
                     /locus_tag="P5179_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS96104.1"
                     /translation="VVSVKGYEPPVGLILVNPATGSRTICWYGSDSDILQPEELDRGA
                     VESSSMLYLDAREPFASVVAAGWAHAAGRKVFIDADNVTEGIADVLPLCDTIIASQNF
                     ALKYFGDSDPERAARKLHERFRVVCGVTAGSAGSYLATPEESFWQPAFEVEVVDTTGA
                     GDVYHGAFAVGLLRGWPLRDIACFASAVAAMKCRELGGRSGIPRFAEACAFLNERGMH
                     GAWEN"
     gene            674..1459
                     /locus_tag="P5179_12620"
     CDS             674..1459
                     /locus_tag="P5179_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HRS96105.1"
                     /translation="MRPIAEIVDAISRRTLTLDGTDHPVQIDENDVSRAYLPLARLVR
                     ERSACVATRRYVVGISGPPGAGKSVTATILAACLEELRTGVVVIPLDGFHYPNEYLRT
                     HRGPDHAGEERSLADLKGWYPTFNVKRALDALEAVRAGQQVRLPVYSRVLHDPVEGGL
                     SIEPSHHVVLVEGNYLFFREGLWNRVYELLDLTVFVTAPREVLIAQLRERHMRGGRSA
                     REAEEKIRKVDEPNMDAILGTRNRAEFVIEGVRGSLAMERKGV"
     gene            1456..2265
                     /locus_tag="P5179_12625"
     CDS             1456..2265
                     /locus_tag="P5179_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96106.1"
                     /translation="MIPTTRIGALEVSRLAIGGNPFSGISHQSRERDLAMRDFFTVAR
                     IKETLAKCEEHGINVLFGRADNHIMRTLVEYWNEGGRIRWFAQTTPERSSLHDVIRQA
                     AGNGASAIYIHGGTAKDFRDTGNWAGLAEAVDLIRSLGLPAGCATHQPDFHSERRAHG
                     VDVDFCLQSLYSLEGRRGKIDQHDEHEQFDDGDRLPALKAIAANPEPTFAYKVLAAGR
                     KDVGRSLTEVVAYLKEQDGILMGMCPDTNPGMVAENASLVSSLLRSLPESV"
     gene            2265..2558
                     /locus_tag="P5179_12630"
     CDS             2265..2558
                     /locus_tag="P5179_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96107.1"
                     /translation="MQLILVLCMATGLGWFIGSRPVLGARLARSGHTVMLVAIALLLF
                     AMGYSLGSRPDIIAQAGRLGIEAVSLALAGASGAVVSVWLSRLWRNARNTRQG"
     gene            2510..3154
                     /locus_tag="P5179_12635"
     CDS             2510..3154
                     /locus_tag="P5179_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HRS96108.1"
                     /translation="MAVAPVEKRAEYQTGVNVTVFVLLCVSSGVLVGLFGRWVIPGVD
                     TAIVALLVVLVFGVGLDLGVRGDALSRVRSLGIRAIVIPVISCVGSLTGVALVASAFW
                     GHPLKDAFALGSAFGWYSLSGPMLTHLGGAELGALGFLSDVLRELLAFLLIPVVAGRI
                     GFAEAVALGGATSMDTTLPLIARTTNGEAAPLAFAHGMVVGLIAPVLIPFWFRF"
     gene            3233..>3369
                     /locus_tag="P5179_12640"
     CDS             3233..>3369
                     /locus_tag="P5179_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS96109.1"
                     /translation="MIRFARTTCDFCGVCVGVCPVDAISLFRAELLIDHAKCISCNFC
                     V"
BASE COUNT          621 a          900 c         1106 g          742 t
ORIGIN      
        1 gggtagtttc cgtgaaagga tatgagccac cggtgggtct cattctcgtt aaccccgcca
       61 ccggcagtcg gacgatctgc tggtacgggt ctgactcgga cattctgcaa ccggaggagc
      121 ttgatcgggg tgctgtggaa tcgagctcga tgttgtatct ggatgcgcga gaaccatttg
      181 cgtcagttgt cgcggcgggt tgggcgcacg cggcggggcg aaaagtgttc attgacgcgg
      241 acaatgttac cgagggaatc gctgatgtac tgccgctgtg cgacacgatc attgcgtcac
      301 agaacttcgc gcttaaatac ttcggcgatt cagatccaga gcgagctgcc cggaagttgc
      361 acgagcgctt cagggtggtg tgcggagtta cggccggatc ggccgggtca tatctggcaa
      421 ccccggaaga atcattctgg cagcccgcgt tcgaagtcga ggtcgttgac actacaggag
      481 caggcgacgt ttaccacgga gcattcgcgg tgggtctatt gcggggatgg ccgctgcgcg
      541 acatcgcgtg cttcgcgtcg gcggtggcgg cgatgaaatg ccgggaactg gggggaaggt
      601 caggaattcc gcgttttgcg gaggcgtgtg cattcctgaa cgagcgggga atgcacggag
      661 cgtgggagaa ctgatgcggc cgatcgctga gatcgtggat gcgatctcga gacgtacact
      721 tactctggac ggcaccgatc atccggtgca gattgacgaa aatgacgttt cccgtgcgta
      781 tttgccgctt gcacggctgg tccgcgaaag atccgcgtgc gtcgcgacaa ggcgctatgt
      841 agttggcatt tcgggacccc ccggtgccgg aaagagcgtc actgcaacca ttctggccgc
      901 gtgtctggag gaattgcgca cgggcgtggt cgtcattcct ttggacggat tccattaccc
      961 gaatgaatac ctcagaaccc atcgaggtcc ggaccacgcg ggagaagagc gatcgcttgc
     1021 cgatttgaag ggctggtacc cgacgttcaa cgtgaagcgc gcgctggatg cactggaggc
     1081 tgttcgggca ggtcaacagg tgcgtctgcc ggtgtacagc cgtgtcctcc acgaccccgt
     1141 ggaaggaggg ctttccattg agccatcgca ccacgtggtg ctggtagaag ggaattacct
     1201 tttcttccga gaaggcctgt ggaatcgggt gtacgaactt ttggacctta ctgtctttgt
     1261 gacggcgcca agagaggttc tcatcgcgca gcttcgcgaa cgccacatgc gcggcgggcg
     1321 ctcggcacgg gaggcggagg aaaagatcag gaaggttgat gagcccaaca tggatgccat
     1381 tctcggaacc aggaaccgcg ccgaatttgt gatcgagggc gtgcggggtt ctcttgcgat
     1441 ggaaaggaag ggagtatgat accaacgacg cgaatcggcg cgctggaagt cagtcggctt
     1501 gccatcggag ggaatccgtt cagcggaatt tcgcatcaaa gtcgggaaag agaccttgcg
     1561 atgcgggact tcttcaccgt ggcccgcatc aaggaaacgc tcgcaaaatg cgaagaacac
     1621 gggataaacg ttcttttcgg acgtgctgac aaccacatca tgcgcaccct cgtagaatac
     1681 tggaacgaag gagggcgaat ccgctggttt gcgcagacta ctcccgaacg atcctccctg
     1741 cacgatgtca tccggcaggc cgcgggcaac ggagcatcgg cgatctacat ccacggcggc
     1801 accgcgaagg atttccgcga taccggcaac tgggcgggcc tcgccgaggc cgttgacctc
     1861 attcgctctc tcggactgcc cgccggctgc gcaacgcacc agcccgattt ccactctgag
     1921 aggcgagcac acggggtgga tgtggacttc tgtcttcagt cgctttacag cctcgagggc
     1981 cgacgcggga agatcgatca gcacgacgag catgagcagt ttgatgatgg ggatcgcctt
     2041 cccgcgctga aggctattgc tgccaaccct gaaccgacat tcgcgtacaa ggtgcttgcc
     2101 gcgggacgga aagacgtcgg gcgttcactg acggaagtcg ttgcctacct gaaagaacag
     2161 gacggaattc tcatggggat gtgccctgac acgaaccccg gtatggtggc ggagaatgct
     2221 tcacttgtga gcagcctgct tcgctccttg ccagaaagcg tctgatgcaa ctgatcctcg
     2281 ttctgtgtat ggcaaccggc ctagggtggt tcatcggaag tcgtcctgtt ctgggcgcgc
     2341 ggctggcgcg gtcgggtcac acggtcatgc tcgtcgcgat tgcgttgctg ttgtttgcga
     2401 tgggttattc gctcggttcg cgtccggaca tcattgcaca ggccggaagg ctgggtattg
     2461 aggctgtgag cctggccctt gcgggggctt ccggagcggt cgtttcagtg tggctgtcgc
     2521 gcctgtggag aaacgcgcgg aataccagac aggggtgaat gtgacagttt ttgtgctttt
     2581 gtgcgtgagc tccggtgtgc tggttggcct tttcgggaga tgggtaatcc ccggcgtcga
     2641 cactgcaata gttgcgcttc tcgtcgtgct tgtgttcggt gtcgggctcg acctcggggt
     2701 gcgtggcgac gcgctttcga gggtgcgctc gctcgggatt cgagcgattg tgatcccggt
     2761 catttcgtgt gtcgggagct tgacgggtgt tgcgctggtg gcgtcggcct tttggggaca
     2821 ccctctcaaa gacgcatttg cacttggaag cgcgtttgga tggtacagtc tttcgggccc
     2881 gatgctgact catttgggtg gagcggaact cggggctctc gggttccttt ccgatgtgct
     2941 gagggaactc ctcgcgtttc ttttgattcc cgtcgtggcg ggccgaatcg gctttgcgga
     3001 ggcagtggcg ctcggagggg cgacatcgat ggacacgacg ttacccctga ttgcccgaac
     3061 gacgaacggc gaagccgcac ctctcgcctt cgcccatggc atggtggtag gcctgatcgc
     3121 gccggtgctc atccctttct ggttccgatt ctaacttgcc accgcgagca gcgaaggatt
     3181 acttttccga acccgtgtga tgagtcccac cgtgacccat cggtgaacag gcatgatacg
     3241 tttcgcacga actacctgtg atttctgcgg cgtctgcgtg ggcgtatgcc ccgttgacgc
     3301 catctccctt ttcagagcgg agttgttgat cgaccacgcg aagtgcattt cctgcaactt
     3361 ctgcgtcga
//
LOCUS       DAOTNG010000329         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22540_length_3333_cov_5.857535, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000329 DAOTNG010000000
VERSION     DAOTNG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22540_length_3333_cov_5.857535"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1849
                     /gene="lysS"
                     /locus_tag="P5179_12645"
     CDS             338..1849
                     /gene="lysS"
                     /locus_tag="P5179_12645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRS96110.1"
                     /translation="MEYVQLLIEQRKAKIEAIRALGVDPYPYRFDPANTTREIVADPE
                     RFSEQPVSIAGRLMSIRGKGKTAFAHIQDAEGRIQIYVRRDAVGETPYSIWNLLDIGD
                     IIGVKGPVFVTHTGETTVQVTEFVVLCKSIRPLPVVKEQMVEGERRVYDDIRSNVELR
                     YRHRSIDLIINPEVREVFRKRSQILQSVRRVLEDRGFIEVETPALQRVYGGANARPFK
                     THHNALDIDLYLKISPELYLKRLVVGGLERVYDLSKNFRNEGIDRTHNPEFTMLEAYQ
                     AYADFNDMMALTEAVYVESCLKVNGTTTISLQGREINLEPPWERVEMVEAIGKHAGIS
                     VENLSDEELLSMVRVHDPDAPAVLSRGMLIADLFEYRVEKELTGPVFVTNHPEETSPL
                     CKASRTRPGFIERFEPYILGYEIGNAYSEMNDPVRQRQLLEAQAAHREVDGEVPPVDE
                     DFLRAMEIGMPPTGGLGIGIDRMVMVLTDQASIRDVIAFPTLRPADARDADTP"
     gene            1869..>3333
                     /locus_tag="P5179_12650"
     CDS             1869..>3333
                     /locus_tag="P5179_12650"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRS96111.1"
                     /translation="MVKTPEAAYEELVQRVKNIALLGNTASVLHWDMETYMPSAGAEY
                     RSQQLSLLSGMLHEQFTAPEIGDLLTCVEQSDLVKDPLSDYAVNVREIRREYDREVKV
                     PKSLVEELARVTSLAHVVWREARKKSDFKAFQPTLENVVRLTLEKADAIATGPTRYDT
                     LLDGYEPGMTTDRMKAVFTALGNELNPFVEEILSSGKEPDRTILTRKYPTDRQAVLGQ
                     ILVGAIGFDLAGGRIDVTTHPFCAQVGLGDVRVTTRYNERDLGDAMFGLLHEAGHGLY
                     DQGTDPAHFGLPRGGFVSLGIHESQSRMWENFVGRSRGFWHFALPIARSVFPEALSDV
                     SLDAFYWAVNDVQRSYIRVEADEATYNLHVLLRFEIEQAIVSGDLAVADIPGAWNEKM
                     QQLLHITPRNDAEGCLQDTHWSGGGFGYFPTYTLGNLYAAQFFEQARADLGDLDTQFR
                     RGDFLPLREWLKEKIHRQGMRYRANELVEKVTGKPLSH"
BASE COUNT          733 a          893 c         1017 g          690 t
ORIGIN      
        1 tttcgccctg gtttccaccc gaattgacag tgcggatttc ccgctcaaca aagtgagacg
       61 agcgtagaca cccggttgga gtcttttcgg atcgggagtt cgcgttctgc gatgtccatt
      121 ttacgccggg cggcttcgat gtgatggccg ctgctcgcca ggtgggtcct ctgagcacac
      181 tgatcccgtt cgccggcaac tacgcctgat tccacgtgca gttggcgtgt tccggccctc
      241 ggcggtacat tttgtttaac cctcccgagt gttcatgggt atctaacgtt tcacgttgca
      301 gtccgtgact cctgtctggg attccgggga ggattccgtg gaatacgtgc agttgttgat
      361 cgagcagcgc aaagcgaaaa tcgaggcgat ccgcgcgttg ggggtggatc cgtaccccta
      421 ccggttcgat cctgccaaca cgaccaggga aattgttgct gatcccgaac ggttttccga
      481 acaaccggta tcaatcgcag gacggctgat gtccattcgg ggaaagggga aaacggcgtt
      541 cgcgcatatt caggatgccg agggaaggat acagatctac gtgcgcagag atgccgtagg
      601 ggaaaccccg tattcgatct ggaacctgct ggacatcggt gacatcatcg gggtaaaggg
      661 gccggttttt gttacccata cgggcgaaac caccgtccag gtgaccgagt tcgttgtcct
      721 ctgcaaaagc attcgtccgc ttcccgtggt gaaggaacaa atggttgagg gggagcggcg
      781 ggtgtatgat gacattcgtt cgaatgtcga actccgctat cgtcaccgct ccatcgacct
      841 gattatcaac cccgaagtgc gcgaagtgtt ccgcaaaagg tcgcagatcc tgcagagcgt
      901 aaggcgcgtt ctcgaggatc gcggttttat cgaggtcgaa acccctgcgc tccagcgggt
      961 gtacggtggg gcgaacgcac ggccgttcaa aacgcatcac aacgctctgg acatagacct
     1021 ctatctcaaa atcagccctg agctctacct taagcgattg gtcgtaggcg gcttggaacg
     1081 cgtgtacgac ttgtcgaaga acttccgcaa cgaaggaatc gaccgcacgc acaaccctga
     1141 gttcaccatg cttgaggcgt accaggcata cgcggatttc aacgacatga tggctctcac
     1201 ggaagccgtg tacgtggaat cgtgcctcaa agtgaacgga acaacaacaa tctcccttca
     1261 gggacgcgag atcaatctcg aacctccgtg ggaacgggtc gagatggtcg aggctatcgg
     1321 aaaacatgcc ggcatttcag ttgaaaacct atcggatgag gaactgctca gcatggtccg
     1381 agttcacgat ccggatgcgc cggccgttct ttcgcggggt atgctgatcg ctgacctgtt
     1441 cgaataccga gtggaaaaag aacttaccgg ccctgttttc gtgacaaacc atccggagga
     1501 gacttcgccg ttgtgcaaag cctcccggac gaggccgggg ttcattgaac gatttgagcc
     1561 gtacatactg gggtacgaaa tcgggaacgc gtactccgag atgaacgacc cggttcgtca
     1621 acgccagctc ctcgaggcac aggcggcgca ccgtgaagtg gacggtgaag tgccccctgt
     1681 cgacgaggat ttcctccgcg caatggagat cgggatgcct ccgaccggag ggttgggcat
     1741 aggcatagac cgaatggtca tggtgctcac cgaccaggcg tcaatccgcg acgtcatcgc
     1801 gttccccacg ttgcgccctg cggacgccag agatgccgac acaccctgac gcagaaacgg
     1861 agcgaccaat ggtgaaaaca ccggaagcag cgtatgagga actcgtccag cgggtgaaga
     1921 acatcgctct gctggggaat acggcaagcg ttttgcactg ggatatggaa acgtacatgc
     1981 cctcggcagg ggcggaatac cgttcgcagc agctatcctt gctctcggga atgctgcatg
     2041 agcaattcac ggcgccggaa atcggtgatc tgctcacatg tgtcgaacag agtgatctcg
     2101 tgaaggaccc cttgtccgat tacgcggtta acgtaaggga aatccgtcgc gaatatgacc
     2161 gtgaagtgaa ggtgcccaaa agccttgtgg aagaacttgc acgggtaacc tcgctcgcgc
     2221 acgtggtatg gcgcgaggcg agaaagaaat ccgatttcaa ggcctttcaa cctacgcttg
     2281 agaatgtggt tcggctgacg cttgaaaagg ccgatgccat cgccaccggg cccaccaggt
     2341 acgatactct tctggatgga tacgagcctg ggatgaccac ggatcgcatg aaagcagtgt
     2401 tcactgcctt gggcaacgag ttgaacccct tcgtcgagga gatcctttcc tccggaaagg
     2461 agcccgaccg gacgatcctg acgcggaaat acccgaccga caggcaggca gttctcgggc
     2521 agatactggt gggtgcaatt ggtttcgatc tggcgggcgg acggattgat gtgacgaccc
     2581 atcccttctg cgcgcaggtc ggtctgggtg acgtcagagt caccacgcga tataacgaac
     2641 gcgatctggg agacgcgatg ttcgggcttc tccacgaagc gggccacggt ttgtatgatc
     2701 aggggacaga cccggcgcat ttcgggcttc cgcggggtgg cttcgtgtcg ctcggtattc
     2761 acgaatcgca gtcgcggatg tgggagaatt tcgtcggaag gagccgtggt ttctggcatt
     2821 tcgcattgcc gattgcgagg agcgttttcc cggaggcgct ttcggatgtg tcgctcgatg
     2881 ccttttactg ggccgtgaat gatgtccagc ggtcgtacat ccgggtagaa gcggacgaag
     2941 caacgtataa cctgcacgtc ctcctgcgtt tcgaaatcga acaggccatc gtctcaggcg
     3001 acctcgcggt ggcggacatt cccggcgcgt ggaacgaaaa gatgcagcaa ctgctgcaca
     3061 tcaccccgcg gaacgatgcc gaggggtgcc tgcaggacac gcactggagc ggaggtggtt
     3121 tcgggtattt ccccacgtat acgctgggaa acctttacgc ggcgcagttt ttcgagcagg
     3181 cgcgggcgga tctcggtgat ctcgacacgc aattccgcag gggagatttc ctgccactga
     3241 gagaatggct caaagaaaag atccaccggc agggaatgcg gtatcgggcg aatgaacttg
     3301 tcgagaaagt tacgggaaag ccgctcagtc atg
//
LOCUS       DAOTNG010000330         3317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22692_length_3317_cov_5.287860, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000330 DAOTNG010000000
VERSION     DAOTNG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22692_length_3317_cov_5.287860"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..2495)
                     /locus_tag="P5179_12655"
     CDS             complement(90..2495)
                     /locus_tag="P5179_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96112.1"
                     /translation="MSVGVERRGDTVVVRAGAYEVVISEAGVVIQHEATQGLTPIARS
                     VRIATAEDNEIPALAGDIVRIWYPDSRCVALEWVYQKGAHTVTTEMRVGCGDSLVSLH
                     ARDAINGEAEVRRFSAMWEFLAGPLNGGRPDFVWTPCLCPEPGQIVGEHVFRSPAAIV
                     QHGEVSFALVPDLSRISAHQPLRSFLDLHCSNSEQGLTCLSSGVCVQKVEGHVFFTTE
                     GVAPVVLRDTSIEFGCDIFVDALAPRMRGHQAVSRMYWKNHGREWLRDIRPQVLPFRG
                     YYEYGYDFADKHLWEETRANEVRVGAMTISREYPHDIWFQGWFNQLRSAYGLFRWARE
                     TGNKERMGRALATRELLFQTPQKNGLFPAIAVLAPADGRPVEWVSSSLQGGGPGVYHL
                     LDSSWTAYWLLQWQLDCEPDVRTLPFCTAYADELVRLQRSDGSFPDYVDVDTHSCVTH
                     YDPTERAARSSSGYVRAMVSRWGTKRLPASAETAIHAMFLAELSRIVVDGEQYFRSAQ
                     DAARYVERNVLPFHKWWDFETFFSCSPKPLDFFDSRSQQYPQNTLSLFWASEAFRILY
                     EITGTAAIGEQSLALTDYLCLFQQVWSPPFLSFYGFGGFGVMNTDGEWNDARQALFAE
                     GLARQYLQSGRQEYLERAVAATRAGFACAYIPENADICPRIFARKPTGYADENYAHGG
                     YDAPAGPSGFDWGIGSALTTAIRMLDWFGDLWVDVYGGWALGIDALTVTSCRREGDVW
                     HLTVDAPLRHTDTITVKGNPGEGEPVCLSLNGQQPVVLTDEALRKGTTVRLYPPGQDP
                     CTR"
     gene            2641..>3317
                     /locus_tag="P5179_12660"
     CDS             2641..>3317
                     /locus_tag="P5179_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS96113.1"
                     /translation="MKWIDFPNEVFVVNGLPWFGETKPHLWRLPERMKGAVRPPVWDL
                     ATNPSGGRIRFRTNTANLSLKANFTTATTYCNISIIGQMGYDIYSDGLYWRSFFPHNA
                     SAPQVGVVEEAIFEKHPREWRDICIYTPIYAPGEVIAFAFDDDAEFLPPAPFAVEKPV
                     VFYGSSITQGGCASRPGMSYQAILGRALNIDFVNLGFSGNGMGEPELAEAMAEIDASC
                     YVVDFAQ"
BASE COUNT          678 a         1066 c          906 g          667 t
ORIGIN      
        1 agcggaatca taatccgcgt gtccggggtt caagtccctg cgccgctact catttcacgc
       61 tcccgcttca tttgaagcgg gggcgttttt catctagtgc acggatcctg tcccggaggg
      121 tacaggcgca cggtcgttcc cttcctcagc gcttcgtccg taagcacaac gggttgctgt
      181 ccgttcagtg agaggcaaac tggttctcct tcgccggggt tacctttcac cgtgatagtg
      241 tcggtgtgtc tcaacggcgc atctacggtc aggtgccaca catcaccttc ccgccggcat
      301 gacgtaacgg tgagcgcgtc aatgcccagc gcccagcccc cgtacacgtc cacccacaaa
      361 tctccgaacc agtccagcat ccgaatcgcg gtcgtaagcg cactaccgat accccagtcg
      421 aacccgctgg gtcccgcagg agcgtcgtaa ccaccgtgcg cgtagttctc gtccgcatac
      481 ccggttggct tccgggcgaa aatcctcggg caaatatcag cattttccgg gatataggca
      541 cacgcgaacc ccgccctggt cgcggcaacg gctcgctcga ggtactcctg ccttccgctc
      601 tggagatatt gcctcgcaag gccctcagcg aacaacgcct gtcgcgcatc gttccactcg
      661 ccatcagtat tcatcactcc gaaaccgccg aatccataga aactgaggaa cggaggagac
      721 cacacctgct ggaacaggca caaataatcg gtaagcgcca gggattgctc gccgatcgcc
      781 gcggttcccg tgatttcgta caggattcgg aacgcctcag aagcccagaa aaggctcagc
      841 gtgttctgcg gatactgctg gcttcgcgag tcgaaaaaat ccaaaggttt gggtgaacat
      901 gaaaaaaacg tctcgaaatc ccaccacttg tggaacggca agacgttccg ctccacgtaa
      961 cgagcggcgt cctgcgcaga acggaaatac tgttctccgt ccaccacaat tcggctcagt
     1021 tccgcgagga acatcgcatg gatcgctgtt tccgcactcg ccggcagccg cttcgtgccc
     1081 catctgctca ccattgcacg tacgtaaccg gaactggacc tcgccgcgcg ttcggtcggg
     1141 tcgtaatgtg tgacacacga atgcgtatca acgtcaacat agtcgggaaa actcccgtcg
     1201 ctccgctgaa gacgaacaag ctcgtccgcg tacgccgtgc agaaagggag tgttcgcacg
     1261 tcgggttcgc agtcaagttg ccactgcagt aaccagtacg ccgtccacga gctgtcaagc
     1321 agatggtaaa ctccgggtcc gccaccttga aggctgctcg atacccattc aaccgggcgt
     1381 ccgtccgctg gcgcgagaac cgcaattgca gggaaaagcc cgtttttctg cggtgtttgg
     1441 aacagaagct ccctcgtcgc gagcgccctc cccattctct ccttgttgcc cgtttctcgt
     1501 gcccagcgga acaacccgta cgccgagcgg agctggttga accatccctg aaaccagata
     1561 tcgtgcgggt attcccggct tatcgtcatg gcgccgacgc ggacctcgtt cgccctcgtt
     1621 tcctcccaca ggtgcttgtc ggcaaagtcg tacccatact catagtagcc gcggaaaggg
     1681 agcacctggg gacggatgtc gcgcaaccat tcccgtccgt ggttcttcca gtacatgcgg
     1741 ctcacggcct gatgcccgcg cattctggga gccagagcat ccacaaagat gtcgcaacca
     1801 aactcgatgc ttgtatcccg caacaccacg ggagcgacgc cttctgtcgt gaaaaagaca
     1861 tgcccttcga ccttctgtac acacacgccg ctggacaaac acgtcagccc ctgctcgcta
     1921 ttcgagcaat gaaggtcgag aaatgaccgc aacggttggt gcgcggagat ccgtgaaagg
     1981 tcgggcacca acgcgaacga cacctccccg tgctgtacga tcgccgccgg gcttctgaac
     2041 acgtgttctc caacaatctg ccccggttca gggcagagac agggcgtcca gacgaaatcc
     2101 ggtcggccgc cgttgagtgg ccccgccaga aactcccaca tggcactgaa gcgtcgtact
     2161 tccgcttcac cgtttatcgc atcccgggcg tgaagactta caagtgaatc tccgcacccg
     2221 acgcgcatct cggtagtgac agtgtgagcc cccttttggt acacccattc aagagcgacg
     2281 caccgggaat cgggatacca gatgcgcaca atgtcgcctg ccagcgctgg aatctcattg
     2341 tcctccgctg tggcaatgcg taccgaacgg gcgatcggag tgagcccctg cgtggcttcg
     2401 tgctggataa ccacaccggc ctcggaaatg actacctcat atgcccccgc tctcacgacg
     2461 acggtgtcgc ctctccgttc cacgcctacc gacatgcccc actccttccg tccggatcgt
     2521 tcctgtccac cagaaaagta ccgcggacac ccgggaacag aaacccggtt gcgtcagcac
     2581 accccccgat ggtttcattt gttgaattgt tcaaccattt tgccacgtgg ggaggttttc
     2641 atgaaatgga tcgatttccc gaatgaggtc ttcgtggtca acgggctgcc ctggtttggc
     2701 gagacgaagc cccacctgtg gcggctgccg gaacgcatga agggtgcggt caggccaccc
     2761 gtctgggatc ttgccacaaa cccgagtggc ggacgcatcc gtttccgcac aaacaccgca
     2821 aatctttccc tcaaggcgaa tttcaccacc gccaccacgt actgcaacat ctcgatcatc
     2881 gggcagatgg ggtatgacat ctactcggac ggcctttact ggcgctcctt cttccctcac
     2941 aatgcctccg ctccgcaggt cggtgtggtg gaggaggcga ttttcgagaa gcatccgcgg
     3001 gagtggcgcg acatctgcat ctacaccccg atctacgcac cgggcgaggt gatcgcgttc
     3061 gccttcgacg acgacgcgga attcctgccg cccgccccgt tcgcggtgga gaagccggtg
     3121 gtgttttacg gttcatccat cacgcagggc ggctgcgcaa gtcggcccgg catgagctat
     3181 caggcgatcc tgggccgcgc gctgaacatt gatttcgtga acctcggctt tagcgggaac
     3241 gggatgggcg agccggaact ggccgaggcg atggccgaga ttgacgcctc gtgttatgtg
     3301 gtggactttg cgcagaa
//
LOCUS       DAOTNG010000331         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22789_length_3307_cov_4.589176, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000331 DAOTNG010000000
VERSION     DAOTNG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22789_length_3307_cov_4.589176"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="P5179_12665"
     CDS             <1..150
                     /locus_tag="P5179_12665"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914077.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HRS96114.1"
                     /translation="LEISAETDGVIMGLRHRQCAIEGVQFHPESILTTEGKAILANFL
                     SLPNR"
     gene            160..849
                     /locus_tag="P5179_12670"
     CDS             160..849
                     /locus_tag="P5179_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRS96115.1"
                     /translation="MAHQFDLVVFDLDGTLCDTAPDIGRCLNRAMADFGLPPVPREKL
                     LKAIGPGGTAFHRAIVPNEEVLPLAEKIVDRYRSYYVTSNTVLTRPFPGIVEMLDDLR
                     ATGLTMAVASNKPQEQTRQIVRGLGLMPYFAEVVGPEAVPRPKPEPDVLHFLIERVGR
                     GCPPGRTAMVGDTDNDMSAGRAAGVRTVFVTWGYMSESEIAPGSIDVVVSSPKELVAV
                     LTESRQKAEAK"
     gene            846..1862
                     /gene="trpD"
                     /locus_tag="P5179_12675"
     CDS             846..1862
                     /gene="trpD"
                     /locus_tag="P5179_12675"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRS96116.1"
                     /translation="MTVSEAIQRLASRTDLTRAETSDAMTQVMSGEATEVQIAGFLIG
                     LRTKGETVEEVAGAADAMRLKATRIPTRHGVLVDTCGTGGDRSGTFNISTTAAFVVAG
                     AGLPVAKHGNRSITSRCGSADLLSGLGVRIDLTPERVGACLDETGIGFLFAPVFHSAM
                     KYALEVRRQLAPTPTVFNMLGPLTNPAGASRQIIGVYRADMVELVARAAALLGTEHTF
                     VVHGSDGLDEITTTGSTKVAEVRNGATSFSEIKPEELGIPLATLGDIRGGDTSTNVEI
                     TRAVLAGAKGPHRDIVTLNAGAAIVAGGLAETLREGILRAEESIDTGRAQSVLRNLVD
                     FTNA"
     gene            1855..2673
                     /gene="trpC"
                     /locus_tag="P5179_12680"
     CDS             1855..2673
                     /gene="trpC"
                     /locus_tag="P5179_12680"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRS96117.1"
                     /translation="MPEDRSMLERMVGAMIAEVECRKSRVPEREIIRKVHDMPAALSF
                     ESVIRRAGFAIIAEFKRASPSRGFIAREASPAQTAHSYQLAGATALSVLTNSEFFHGS
                     DDDLSAIRQTTTIPVLRKDFTVSSYQIYEARAIGADAILLIVAALDDYQLRDFLALAR
                     TVGLSAIVETHSEEEIERACGAGASIIGINNRDLKTFETTIQTTLSLAPLVPEDIAVI
                     SESGIHSGKDAALLRQAGVHGILVGESLMRAAQVSQDHLVRHMHELFGRSELRS"
     gene            2739..>3307
                     /locus_tag="P5179_12685"
     CDS             2739..>3307
                     /locus_tag="P5179_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRS96118.1"
                     /translation="MEEAKIYLSEAEMPRHWYNIVADMPNKPAPPRHPATGEPVPPEA
                     FSAIFPMNIIEQEISAEREIAIPEEVLSFYRMYRPTPLIRAFRFEKALKTPAKIFYKY
                     EGASPAGSHKLNSAIAQVYYNKQAGTKRITTETGAGQWGSALAMACNFFGLECVVYMV
                     RVSYNQKPYRRIVMETFGAKVHASPSNNT"
BASE COUNT          769 a          931 c          980 g          627 t
ORIGIN      
        1 ctcgaaatca gcgcggaaac ggacggtgtg atcatgggac tgcggcaccg tcagtgtgcc
       61 atcgagggag tccaattcca tccagagagc attctgacaa ccgagggaaa agcgatactg
      121 gccaatttcc tttctcttcc gaaccgttaa ggacaacgca tggcacacca attcgatctc
      181 gttgttttcg atctcgatgg aacgctctgc gacacggcac ctgacatcgg gcgttgcctg
      241 aatcgggcga tggcggattt tgggttgcca cccgtgccac gggagaaact cctgaaagcg
      301 atcgggcccg gtggcacggc atttcaccgt gccatcgttc ccaatgaaga ggtcttaccg
      361 cttgccgaaa agatcgtcga ccgttacagg agctattacg taacgagcaa cacggtgctc
      421 acgagaccct tcccgggaat tgtcgagatg ttggacgact tacgtgccac gggccttaca
      481 atggcagtgg caagtaacaa acctcaggag cagacgcggc aaatagtgag agggttggga
      541 ctcatgccgt acttcgccga agtcgtcggg cctgaggctg tgccgcggcc taaaccggag
      601 cctgacgttc tccatttcct cattgagcgc gtcggacgag ggtgcccgcc tgggagaacg
      661 gcaatggtcg gagatacgga taacgacatg agcgcgggtc gcgctgccgg tgtgcggacg
      721 gtgttcgtga cgtggggata catgtccgaa agcgagattg cacccggcag catagacgtc
      781 gtcgtgtcct cgccgaagga actcgtcgca gtactcacag agtcccggca gaaggcggaa
      841 gcgaaatgac ggtttcagaa gcgattcaga gacttgccag tcggacagac cttacccggg
      901 ctgaaacctc ggacgcaatg actcaggtga tgtcgggtga ggccacagag gttcagattg
      961 cgggatttct gatcgggctt cgtaccaagg gcgaaacggt ggaagaggtt gccggcgccg
     1021 cggatgcgat gcgcctcaaa gcaaccagaa tcccgacgcg gcatggcgtt ctcgtggata
     1081 cgtgcggaac cggcggagat cgatcgggca cgttcaacat ctcgacgacg gcagcatttg
     1141 tggttgcggg ggccggcctt cccgtggcaa aacatggcaa tcgttcgatc accagcaggt
     1201 gtggctcggc cgatctcctc agtggcctcg gcgttcggat tgatcttacc ccggagcggg
     1261 tgggcgcgtg tctcgacgaa acaggtatcg gattcctctt cgctccggtg ttccattcgg
     1321 cgatgaaata cgctcttgag gtccgccgtc agcttgctcc aacgcctacc gtgttcaaca
     1381 tgctcggccc gttgacaaac cctgcggggg cttcacggca aatcatcggc gtgtaccgtg
     1441 cggacatggt ggaactcgtc gccagagcag cggctcttct cggaacggag cacacgtttg
     1501 tcgttcacgg ttcggacgga ttggacgaaa tcacaacgac agggagtacg aaggtcgcgg
     1561 aagtcaggaa cggtgcaacg agcttctccg aaatcaaacc ggaggagctg ggaatacctc
     1621 tggcgactct gggggatatt cgcggtggtg acacctcaac aaatgttgaa atcactcgcg
     1681 ccgttctggc aggagcaaaa gggccgcacc gggatatcgt taccctgaac gcgggtgcgg
     1741 cgattgttgc aggaggactg gcggaaaccc tgagggaagg catcctgcgt gcagaggaat
     1801 caatcgacac cgggcgcgcg cagtctgttc tgcgaaacct tgtggacttc acaaatgcct
     1861 gaagaccgat caatgctgga acggatggtg ggagcgatga tagctgaagt cgaatgccgc
     1921 aagtctcgag ttccggaacg tgagataatc cggaaagttc acgacatgcc tgctgccctt
     1981 tcctttgagt cggtcatcag gcgcgcgggg tttgcgatca tcgccgagtt caaacgggct
     2041 tcgccgtcac gagggttcat cgcccgggag gcatcgcccg cacaaaccgc gcactcgtat
     2101 caactggcag gcgcaacagc tctcagcgtt ttgaccaatt cggagttttt ccacgggtca
     2161 gatgacgacc tttccgccat caggcagaca accaccatcc cggtacttcg gaaagacttc
     2221 accgtgagct cgtaccagat ctacgaagcc cgggctatcg gggccgacgc aatactgctc
     2281 atcgtagcag cactagacga ttaccagttg agagactttc ttgccctggc gcgaaccgtc
     2341 gggctctccg cgatcgtgga aacccattcc gaagaagaaa tcgaaagggc gtgcggcgca
     2401 ggcgccagca tcatcggcat caacaaccga gacctcaaaa ctttcgaaac taccatccaa
     2461 acgacccttt cgctcgcgcc gctggtaccc gaagacatcg ccgtgatcag tgaaagtggg
     2521 attcactccg gcaaggacgc cgccctgctc aggcaggcag gtgtacacgg tatcctcgtt
     2581 ggagagtcac tcatgcgcgc agcccaggtt tcacaggatc accttgtccg tcacatgcat
     2641 gagttgttcg gtagaagcga attgcgatca taagaacatc aaccgcgcgg ggcctttgcg
     2701 gcgccgccac accacacaaa aaagtggagg aacgcagaat ggaagaagcc aaaatctacc
     2761 tgagcgaggc cgagatgccg cggcactggt ataacatcgt cgccgacatg ccaaacaagc
     2821 cggcgccacc tcgccaccct gcgacgggcg aaccggttcc cccggaggct ttcagcgcca
     2881 tattcccgat gaacatcatc gagcaggaaa tcagtgctga acgggagatc gccattcctg
     2941 aggaggtctt gtccttttac aggatgtatc gcccaacccc tctgatccgg gcattccggt
     3001 tcgaaaaagc gctcaagacg cctgcgaaga ttttctacaa atatgagggc gcgagcccgg
     3061 caggcagcca caagctgaat tccgccatcg cacaggtgta ttacaacaaa caggcgggaa
     3121 caaaacgcat aaccacggaa acaggagccg ggcagtgggg cagcgccctg gccatggcgt
     3181 gcaatttctt tggtctggaa tgcgtcgtgt acatggttcg cgtaagctac aatcagaaac
     3241 cttaccgcag aatcgttatg gaaaccttcg gcgcgaaagt gcatgcgagc ccatccaaca
     3301 acacgaa
//
LOCUS       DAOTNG010000332         3299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22848_length_3299_cov_5.231196, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000332 DAOTNG010000000
VERSION     DAOTNG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22848_length_3299_cov_5.231196"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="P5179_12690"
     CDS             <1..1278
                     /locus_tag="P5179_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRS96119.1"
                     /translation="MLDNFLSLCGCARTWTMEQFITQAEERIRRQAGDRNVFMLVSGG
                     VDSSVAFVLLSRALGEHRVIGLHIDNGFMRLNESVLVKESLERAGFHNLHVVDASEKF
                     LAAVKGVTDPQQKRQIVGDTFISVQQEELERIGLDPDHWMLGQGTLYPDIIESGGTRH
                     ASVIKTHHNRVAIIRQMLEEGKIIEPLDQLYKDEVRQVGRQLGLPDALVERHPFPGPG
                     LSVRCLCSDGVEEPIEASAKEKVRALAGSLGFESEILPVRSVGVQGDERTYRHPVALW
                     RPDGGAVNWEELERLSTRITNTVPEVNRVMLLVYPRSLPRLKLRARFLDRPRLDLLRL
                     ADHAAMSVLVEDGLAGDFFQFPTILIPLGEREEGESVVLRPLESTDVMTASFAILPES
                     SLQRLVSRLAALPVVDAVFYDITHKPPATMEWE"
     gene            1375..1692
                     /locus_tag="P5179_12695"
     CDS             1375..1692
                     /locus_tag="P5179_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96120.1"
                     /translation="MYHATKAEFALTGATARLYEIFLDATRGSAAVGDVNRALFETGL
                     VHHALMLLALGVVPEERAKEARALIDEIGKTTIMKDSFDQAREYWERVAKVNPSAPES
                     SDG"
     gene            1685..2740
                     /locus_tag="P5179_12700"
     CDS             1685..2740
                     /locus_tag="P5179_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS96121.1"
                     /translation="MDEAVSAALRANGYLWDLYTRREEYEPIFLDGLGRFRFFASQQR
                     APFDPVVSRFLFERGAFPAFPAGRRYAICLSHDIDALHLNRYRSGGAGLLSRARRAGA
                     RSAELLLSRSLKRGDPGWNLARIADIEKERGAKSAFYSLALEEGEKDFYFRVQEIGAQ
                     LRTLVARGWEAGLHGGHEAYCDPTALRREKARLEAELGTPVTGFRAHYLRFDVPGTWR
                     LLAEAGFRYDTTFGYADCVGFRNGMCHPYTPFDRNADRFIPILEVPLAVMDTTLQLYM
                     RLDPADAWTVIRRLLDAVAECSGVLTVLWHPQNMNGAWGDLYLRILDYGTDTDAWLTG
                     PGELAGWWLSQQAGGNA"
     gene            2794..>3299
                     /locus_tag="P5179_12705"
     CDS             2794..>3299
                     /locus_tag="P5179_12705"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HRS96122.1"
                     /translation="MVLVDKGAPCSVVVSGAQPTWLERHAAEELVHYLRLISGAALPT
                     VSGIPDGMNAVVIGRADTNSVVAEAVHEGVLSLSEDSPGWDGFVIKTARRGNRDVCFV
                     GGSSDRGTLYAVYWLLEEVLGVGFFRDGEYIPRSDSVVLPESLHIVERPVFEHREDGL
                     LAYSAKFWG"
BASE COUNT          592 a          899 c         1137 g          671 t
ORIGIN      
        1 atgctggaca atttcctttc tctgtgcgga tgcgcgcgca cctggacgat ggagcaattt
       61 atcacgcagg ccgaggagcg catcaggagg caggccggcg acaggaacgt gttcatgctt
      121 gtatcgggcg gggtggattc gtccgtggcg tttgtgctgt tgagcagggc actgggggag
      181 caccgtgtca tcggtctcca cattgataac ggcttcatgc ggctgaatga atcggtgctg
      241 gtcaaggaat cgcttgaacg tgcggggttc cacaacctgc acgtagtgga cgcgagcgag
      301 aagttcctcg cggcggtgaa gggggtgacg gatccccagc agaagcggca gattgtgggc
      361 gacacgttca tcagcgtgca acaggaggag cttgagcgga tcggcctcga ccccgatcac
      421 tggatgctgg gccagggcac gctctacccg gacattatcg aatcgggggg aacccggcac
      481 gcgtcggtga tcaagacgca ccacaaccgt gtggcgatca ttcgtcagat gctggaggaa
      541 ggcaaaatca tcgagccgtt ggaccagttg tacaaggatg aagtgcgaca ggttggacgg
      601 cagctcggtc tgcccgacgc gctggtggag cgccacccgt ttccggggcc ggggctttcc
      661 gtccggtgtc tctgttcgga cggcgtggag gaaccgattg aggcgtcggc gaaggagaag
      721 gttcgcgcac tggcggggag cctcggattt gaaagcgaaa tcctgcccgt caggagtgtt
      781 ggcgttcagg gggatgaacg gacgtaccgg caccccgtgg cgctgtggcg cccggacggc
      841 ggcgcggtga actgggagga actggaacgc ctttcaacgc gcatcacgaa caccgtcccc
      901 gaagtgaatc gcgtgatgtt gctggtgtat ccgcgttcgt tgcctcggct gaagttgcgc
      961 gcacggtttc tggaccggcc ccggctggat ctgctgcggc tggccgatca cgccgcaatg
     1021 agtgttctcg ttgaggatgg gcttgcgggg gattttttcc agttccccac catccttatt
     1081 cccctcggcg agcgcgaaga aggcgaatcg gtggtgctgc gcccgcttga atccaccgac
     1141 gtgatgacgg cgagtttcgc gattcttccg gagagttcgt tgcagcggct ggtttcgcgg
     1201 cttgcggcgc ttcccgtggt ggacgcggtg ttttatgata tcacccacaa gcctccggcc
     1261 accatggagt gggaataacg cgcggcagaa gggtccggcg gagaccaccc ggcaccccga
     1321 ttgatgccgg ccggggacac cgtcccgtcg gaagatcaga aggtgaggtg gcgtatgtac
     1381 cacgcgacaa aggcggagtt cgcgttgacg ggagcgacgg cgcgcctgta cgagatcttt
     1441 ctggacgcga cgcgcggttc cgccgcggtt ggtgacgtaa acagggcgct gttcgagaca
     1501 ggcctcgtgc accacgcgtt gatgttgctt gcccttggcg tggtgccgga agagcgtgcg
     1561 aaggaagccc gcgctctcat tgatgagatc ggcaagacca cgatcatgaa agacagcttc
     1621 gatcaggccc gcgaatactg ggagcgggtt gcgaaggtga atccttcggc gcccgagagc
     1681 agtgatggat gaggctgtat ctgccgctct gcgagcgaac gggtacctgt gggatctgta
     1741 cacccgccgg gaggaatacg agccgatttt cctcgacgga ctgggaagat ttcggttttt
     1801 cgcgtcgcaa cagcgggcgc cgtttgatcc cgtcgtctcg cgttttcttt ttgaacgtgg
     1861 cgcatttccc gcttttcccg ccggcagacg ctacgcaatc tgcctctccc acgatattga
     1921 cgcactccat ttgaatcggt accgcagcgg cggagccggt ctcctgagcc gtgcaaggcg
     1981 cgcaggtgcg cgttccgctg aactgctcct ttcccggtcg ctcaagcgag gtgatccggg
     2041 atggaatctg gcgcgcatcg ccgacatcga aaaagaacgc ggcgcgaaga gcgcgttcta
     2101 ctccctcgcg ctggaggaag gcgaaaaaga cttttacttc cgggttcaag agattggcgc
     2161 gcagcttcgg actctcgtcg cacgagggtg ggaagcgggg ctgcacggcg ggcacgaggc
     2221 atattgcgat ccgacggcac tgaggcggga aaaagcccgc cttgaggcgg aactcggaac
     2281 gccggtgacc gggttccgcg cgcattatct gcgcttcgat gttcccggga cgtggcggtt
     2341 actggcggag gcaggtttcc gttacgatac gacgttcggg tatgcggact gtgtgggctt
     2401 tcgaaacggg atgtgccacc cctatacccc gttcgaccgt aacgctgatc gtttcattcc
     2461 gattctggaa gttcccctcg cggtgatgga taccacgctt caattgtaca tgcggctcga
     2521 ccccgcggac gcgtggaccg ttatccggcg tcttctggac gcggtggcag aatgcagcgg
     2581 cgtgctgacg gttctctggc atccgcagaa catgaacggc gcctggggcg atctctacct
     2641 gcgcatactc gattacggca cagacacaga tgcatggctt accggacccg gcgaacttgc
     2701 cggatggtgg ctttctcagc aggcgggggg caacgcctga ggtatcgtgc cgcgtgacgg
     2761 cataccgtac gcggtgcaac aggaggactc gagatggttc tggttgacaa aggcgcgccg
     2821 tgcagcgtgg tagtgtcggg cgctcagccg acgtggctgg aacgccacgc cgcggaagag
     2881 cttgtccatt acctccgcct gatcagcggt gcggcccttc ccacggtttc gggtattccg
     2941 gacggcatga atgcggtggt aatcgggagg gcggacacca attcagttgt cgccgaggcg
     3001 gtgcatgagg gcgttctttc tttgtccgaa gactcaccgg gctgggacgg tttcgtgatc
     3061 aaaaccgcgc gccgtggcaa ccgcgatgtg tgttttgttg gcggaagcag tgaccggggg
     3121 acgctgtacg cggtttactg gctgctggaa gaggtgctcg gggtgggctt cttccgggat
     3181 ggcgagtata tcccgaggag tgattctgtt gtgctgccgg aatcgctgca cattgtggaa
     3241 cggccggtgt ttgagcacag agaggacgga ttgctggcgt attcggccaa gttctgggg
//
LOCUS       DAOTNG010000333         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22885_length_3295_cov_5.762963, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000333 DAOTNG010000000
VERSION     DAOTNG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22885_length_3295_cov_5.762963"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="P5179_12710"
     CDS             <1..922
                     /locus_tag="P5179_12710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96123.1"
                     /translation="YPFRRLGGYPTLVNLEVTKKCNARCDFCDYWKTESEERLSDYAP
                     VIARLRPTVVMLTGGEPLLRRDLEAVIAGIKKQSPAVYLGLITHAAMLTPKRGLALWE
                     AGLDQLSISLDFLDERHDKARGIPGLVKKVTETIPLLVEAGIDNLALNTVIKSDNLDQ
                     VVAMVHQCEDWGIGIAFSAYADVKVGNKEHNIRDAQMRELQRVIDELLRLKREGAPIL
                     SSSYYLSRVPEFFATGHIGGCVAGDRFVTVTPSGHVKRCSEFDAEVHFSEWTPKSIGT
                     TDCDVCWFSCRGESQAPVISLERLKDTLLK"
     gene            1046..2161
                     /locus_tag="P5179_12715"
     CDS             1046..2161
                     /locus_tag="P5179_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96124.1"
                     /translation="MTQYERVRAALLGEPADRPPVIGGWVCQEDFLVTMSHVTRDEFW
                     KNPYAHATTAYKNAGADALVQFVLPKRETEATQGEYGRPTNFGKQEERAEDRFPTLES
                     VIEWVRKQPTVDEVRRTFDAKKAYDQYLEVVHERDDIAPMVWIPGHVCGCPPFMWYSY
                     LGYENYFAGMMEAPEVFERFFAMEGEQRRLQNAEVARAIRENGLLPLLYFGEDICYSS
                     GPMCSPKLLREIYFPHLARAMEPLVEAEIISVWHSDGNIMPIIDDLIRCGVDGFQGLE
                     EDHGMSLETLAGMTNRNGVPLLLWGSVSVTSTLPHGSVEDVRKDVKRCIDIGARRGGR
                     FFLAPSSSVGPEVPTENIVELFRFGREYGLEAYGKGR"
     gene            complement(2304..3263)
                     /locus_tag="P5179_12720"
     CDS             complement(2304..3263)
                     /locus_tag="P5179_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96125.1"
                     /translation="MKPLLLHYYVTYRCNARCSFCTIWDPAKWDHRATPSWDQMTSNL
                     TAARKLGTRFVDFTGGEPLLYHDLPRALRLAKVLGFRTSVTTNCLLYPARAKDLAGQV
                     DLLHFSLDAAEKETHDRIRGVPCFDRVMESLDIARALGEHPDILFTATEETFEQIAPL
                     AAIAQAYNTMLIVNPEFAWAGNKGVGERELACLERFARERNVYVNRAFHRLIRAGGNQ
                     RAHPVCHAVEAVIVISPDDHLLLPCFHRQRERIPIDGQLEKVRASSLVKAYKREDGRQ
                     VYCQGCTINCYMDPSFQYACNAYLFLSLAAKAKYAWSKYVLLA"
BASE COUNT          669 a          840 c         1130 g          656 t
ORIGIN      
        1 gtacccgttt cggcggcttg ggggatatcc gacgctcgtc aatctggaag tgacgaagaa
       61 gtgcaacgcg cggtgcgatt tctgcgatta ctggaagacg gagtcggagg agcggctcag
      121 cgattacgct cccgtaatag cgagactgag accgacggtg gtgatgctta ccggcgggga
      181 gccgttgctt cggcgcgacc tggaggcggt aatagccggg ataaagaaac agtcgccggc
      241 ggtgtacctc ggcctgatca cccacgcggc gatgctgacg ccgaaacggg gtctggcgct
      301 ctgggaggcg gggctggatc agctttcgat cagtctcgac ttcctcgatg agcggcacga
      361 caaggcgcgt ggaataccgg ggctggtgaa aaaagtgacg gagacgatcc cgcttcttgt
      421 tgaggcggga attgataacc ttgcgttgaa cacggtaatc aagtcggaca accttgatca
      481 ggtcgtggcg atggtgcatc aatgcgagga ctggggaatc gggatcgcgt tcagcgcgta
      541 tgctgacgtg aaggtcggca acaaggaaca caacatccgt gacgcgcaga tgagggagct
      601 gcaacgggtt attgatgagc tgttgcggct gaaaagagag ggtgcgccga tactgtcgtc
      661 gtcgtactac ctttcccgcg tgccggagtt tttcgccacc gggcacatcg gcgggtgcgt
      721 tgccggggat cggtttgtca ccgtaacgcc gagcggccat gtgaagcgat gcagtgaatt
      781 tgacgcggag gtccacttct ccgaatggac tccgaagtcc atcgggacga cggattgcga
      841 cgtctgctgg ttcagttgcc gcggagaatc tcaggcgccg gtgatatccc tggagcggct
      901 gaaagacacg cttctcaaat agcgcaccca cgccgaagac cgaatccggt tctgcgctcc
      961 acccggctgt ttgcccatcc ctctcctttc tacgtttatt cccttaccgc aaccggtgag
     1021 ccacctcatt tcgtgcgagg catcgatgac ccagtacgag cgcgtgcgcg cggcccttct
     1081 cggagaacct gcagaccgcc ctcccgtaat tggcggctgg gtatgtcagg aagactttct
     1141 cgtcaccatg agtcacgtga cgcgggatga gttctggaag aacccttacg cgcacgccac
     1201 gaccgcgtac aagaacgcgg gtgcagatgc gcttgtgcag tttgtgcttc cgaaacggga
     1261 gaccgaggcg acgcaaggtg agtacgggcg gcctaccaac ttcgggaaac aggaggaacg
     1321 ggccgaagac cgatttccca cgcttgaatc ggtgatcgag tgggttcgga agcaaccgac
     1381 cgttgacgag gtgcggcgca cgtttgacgc gaagaaggcg tatgaccagt acctcgaggt
     1441 cgtccacgag cgggacgaca ttgcgccgat ggtgtggata cccgggcacg tgtgcgggtg
     1501 cccaccgttc atgtggtatt cgtatctggg atatgagaac tatttcgccg ggatgatgga
     1561 agctcccgag gtgttcgagc gatttttcgc gatggaaggg gagcagcggc ggctccagaa
     1621 cgcggaggtg gcacgggcga tacgggagaa cgggttactt ccgctcctct attttgggga
     1681 ggatatctgc tacagcagcg gcccgatgtg cagcccgaag cttctgcggg agatttattt
     1741 tccgcacctt gcacgcgcga tggaaccgct tgtggaggcg gagatcattt cggtgtggca
     1801 ctcagacggc aacatcatgc cgatcataga tgaccttatc agatgcggag tagacgggtt
     1861 tcaggggctg gaagaagacc acggaatgag ccttgaaacg ttggccggga tgacgaaccg
     1921 gaacggtgta ccgctgttgc tctggggaag tgtcagcgtg acctcgacgc tgcctcacgg
     1981 aagtgtcgag gacgtaagga aagatgtcaa acggtgcatt gacatcggcg cgcggcgtgg
     2041 tggcaggttt tttctcgcgc cttccagttc ggtcggcccc gaagttccga cagagaacat
     2101 cgtggagttg ttccgcttcg ggagggagta cggtctggag gcgtacggga aggggcggtg
     2161 aggaggcgtg tgctagggaa tgtggcatga aggcattgga gcgaacaggt cgaatagggc
     2221 aaatagggcg gatatgtcag aaggagagcg ggggaacagg tggcacgggg gcaatggcgt
     2281 gccacgagcc ggtgctggag ggttcaggcc agaaggacgt acttgctcca cgcgtatttt
     2341 gcctttgccg ccaggctgag gaaaagatag gcgttgcatg cgtactggaa cgatgggtcc
     2401 atgtagcaat tgatcgtgca accctgacag tacacctgcc tgccatcctc gcgtttgtag
     2461 gccttcacga gcgaacttgc ccggactttt tccaactggc cgtctatcgg tatgcgttct
     2521 ctctgccgat ggaagcacgg caggagcagg tgatcgtccg gtgagatgac gatgacggct
     2581 tccaccgcgt gacagacggg gtgcgcgcgt tgattgccgc cagctcgaat gagacgatga
     2641 aacgcccggt tcacgtagac gttccgctcg cgggcgaaac gttcaaggca ggccagttcc
     2701 cgttctccga cacctttgtt ccccgcccac gcaaactccg gattcacgat gagcatggta
     2761 ttgtacgcct gagcgatggc ggcaagaggg gcgatctgtt cgaacgtctc ctccgttgcc
     2821 gtaaagagga tgtcgggatg ttcgccgaga gcacgggcga tgtcgaggct ctccatcacg
     2881 cggtcgaaac atggcacgcc acggatgcgg tcgtgagttt ccttttccgc ggcgtcgagc
     2941 gagaagtgga gcaggtcaac ctggccggcg agatcttttg cacgggcagg gtagagcaga
     3001 cagttggtgg tgacgctggt acggaacccg agcacctttg cgaggcgaag cgcgcggggc
     3061 aggtcgtgat acaggagggg ttcgccgccg gtgaaatcta cgaatcgagt gcccagtttc
     3121 cgggcggcgg tgaggttgga ggtcatctgg tcccaggagg gcgtggcgcg gtggtcccat
     3181 tttgccgggt cccagatggt gcagaatgag caccgtgcgt tgcagcggta ggtcacgtaa
     3241 tagtggagca ggagcggttt cattttgcag cgggaggatc agcgggcgcg aggga
//
LOCUS       DAOTNG010000334         3285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_22988_length_3285_cov_4.610217, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000334 DAOTNG010000000
VERSION     DAOTNG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22988_length_3285_cov_4.610217"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="P5179_12725"
     CDS             <1..512
                     /locus_tag="P5179_12725"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRS96126.1"
                     /translation="GEMWMAATIAAAFAVNDVEQAIRIGLSEVPAECRLAQALNETLA
                     WCNADGALETTLDRIQEEFGHYHPVHTINNAALVVAGLMYGRASLGATICGAVTGGWD
                     TDCNGASAGSVFGAMHGANGLPYEWVGCLNDTLHSALSGVNQGKPHRFTDLAQRTLVR
                     HKKVAARLE"
     gene            515..1759
                     /locus_tag="P5179_12730"
     CDS             515..1759
                     /locus_tag="P5179_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS96127.1"
                     /translation="MSRNTMYALLAMLGIAAGTLVWRQWSYKRATGTQQALTRILEQT
                     DENPGERIRLLDSIMAAHPDDATIRRVAVYQKLRAHVELRSDSATVVRAVNDFLSTDT
                     SGAAFNFASSVYADLAINAPEGLRYAHRALAEVREVKRPKDMTEAQWAQQRRMMVGEC
                     HHILGRLYALSGQSRPALAALRAAADSVPDSPVILLRMAEIHERMGQLDSALESYMQA
                     LRLSHADTSALAGFARLYPAVHGPAVDPATVLDTLVAHARAKRRADLLAQRISQPAAP
                     FDLSTLAGRQYRLSDFRGKTLLLYFWATWCGPCKRQLPAIQEAHTRLKAHKDIELLTV
                     SFDQHFEVVPPFLARKRYDFPVVPGDEGLYKSYLLEGLPTCAVVDSTGTIRFKRLGNS
                     DAGDFLEELGWQLEAVSGKAPW"
     gene            1980..2561
                     /locus_tag="P5179_12735"
     CDS             1980..2561
                     /locus_tag="P5179_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS96128.1"
                     /translation="MPGTGILLRRAQQDGRPAFDRLQRSMEPRLRRFITRLVGNGDRV
                     RDIVQDTFLALYIKLERIVSEEHLWPFVFRVARNLCYDELRRQNRFEIIAVSHGSEHS
                     DDSFIPMATGRRADDITHWVMAVSLVKNAVDQLPEAQRQTLILHFEENLTYEQTAEAM
                     GVDIGTVKSRIHHARKTLRDILPEGFVRALGFE"
     gene            2572..3027
                     /locus_tag="P5179_12740"
     CDS             2572..3027
                     /locus_tag="P5179_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96129.1"
                     /translation="MAVSGEEKIRGLIRLLPVARALKQDLEKALGMEVFSGARNMAAK
                     SYEGLRQAAVALTDDPYLNALELEVFPTATDKEKTAIVALAAGQLLAYLEGQTGVGAV
                     SGGGNYHFSVQSAPNVNGVNLRNVDSRIAARLLGSEIEEEDSEEDDEKE"
BASE COUNT          665 a         1013 c         1031 g          576 t
ORIGIN      
        1 acggcgagat gtggatggcc gccaccatcg ccgccgcatt tgcggtgaac gacgtggagc
       61 aggccatccg aatcggtctg agcgaagtac cggccgaatg ccgtctcgcg caggcgctga
      121 acgagacgct cgcgtggtgc aacgccgatg gcgccctcga aacgacgctg gatcggattc
      181 aggaggagtt tggccactac catccggtgc acaccatcaa caacgccgcg ctggtagtcg
      241 cggggctgat gtacgggcgg gcctcccttg gcgcaacgat ctgcggcgca gtaactggcg
      301 ggtgggacac cgactgcaac ggtgcctcgg ccggatccgt gttcggggcc atgcacggtg
      361 cgaacgggct gccgtacgag tgggtaggat gcctgaacga cacgctgcac agcgctcttt
      421 ccggcgtcaa ccaggggaaa ccacatcgct tcaccgatct cgcacagcgc acgcttgtgc
      481 gacacaaaaa agtcgctgca cgcttggagt agtcatgagc cggaacacga tgtacgcact
      541 gctcgccatg ctgggaattg cggcgggaac gctcgtctgg cgccagtggt cttacaaacg
      601 agcaaccgga acccagcagg ctcttacgcg cattctggag cagacggacg agaacccggg
      661 ggagcgcatc cgccttctcg acagcatcat ggcggctcac ccggacgacg caacgatccg
      721 ccgcgtcgcc gtgtaccaga agctccgggc ccacgtggaa ctcaggagcg actccgcgac
      781 ggtagttcgc gccgtcaacg acttcctgag caccgacacg tcaggcgcgg cattcaactt
      841 cgcgtcgtcg gtatacgccg acctcgcgat caacgccccg gaagggcttc ggtacgccca
      901 ccgggctctt gcggaagtgc gcgaggtcaa acgccccaag gacatgaccg aggcgcaatg
      961 ggctcagcag cgccggatga tggtgggcga gtgccaccac atactcggcc gtctgtacgc
     1021 cttgagcggg caatctcgtc ccgcgctggc ggcgctgcgg gcggcggcgg atagtgtccc
     1081 cgattcgccg gtaattctgt tgcgcatggc ggaaatccac gaaagaatgg gccaactcga
     1141 ttctgctctt gagtcgtaca tgcaggcgct ccgcttgagc catgctgata cgtcggcgct
     1201 cgcgggcttt gcccgcctct accccgccgt tcacgggcct gcggttgatc ccgccacggt
     1261 gctggatact ctggttgcac acgctcgtgc aaaacgcagg gcggaccttc tggcgcaacg
     1321 catttcgcag cccgccgcgc cgttcgacct ttccacgctg gcgggtcggc agtaccgcct
     1381 cagcgacttc cggggcaaaa ccctgctgct ctacttctgg gcgacctggt gtgggccctg
     1441 caagcggcaa ctgcctgcca ttcaggaggc gcacacacgg ctcaaagcgc acaaggatat
     1501 cgagcttctc accgtcagtt tcgaccagca ctttgaggtg gtgccaccgt tcctggcgcg
     1561 gaaacggtac gatttccccg tggtgccggg ggatgagggg ctgtacaaat cctatctcct
     1621 ggagggcctc cccacatgtg ccgtggtgga ttccaccggc acgatccggt tcaagcgcct
     1681 cgggaacagc gacgcagggg acttccttga agaactcggc tggcagcttg aggcggtgtc
     1741 cggaaaagcg ccatggtgac ccgctgacct cccccgaacg aggaaacggc catccggccc
     1801 gcttcctgcg ccctaggccg cctgtccggg caccacgctg acatcagtcc acaggtccca
     1861 tcggacgcgt acctgtcaac tgcgccggta gtctacgaga tccgggccat tgccccactt
     1921 tgaacaattg gccttcctcg ttcggataac cctacagagc catgcaggaa aggcgttgaa
     1981 tgccgggcac gggaatactc ctcagaaggg cacagcagga tggacgcccc gctttcgacc
     2041 ggctgcagag gagcatggag ccccggctcc gcaggttcat cacccgtctc gtcgggaatg
     2101 gagaccgagt ccgggacatc gtgcaggaca cgtttttggc gctgtacatc aaactcgaaa
     2161 ggatcgtctc tgaagaacac ctgtggccgt ttgtcttccg cgtcgcacga aacctctgtt
     2221 acgacgaact gcggcggcaa aatcggttcg agattatcgc tgtcagccat ggttcggagc
     2281 attctgatga ctctttcatt ccgatggcaa cagggcgccg cgcggacgac ataacgcact
     2341 gggtcatggc cgtctctttg gtgaaaaatg ccgtggacca acttcccgaa gcgcaacgcc
     2401 agacgcttat cctgcatttt gaggaaaacc tcacgtacga gcagactgcg gaagcgatgg
     2461 gcgtggacat cggcacggtc aaatcgcgta ttcatcacgc acggaaaacc ctgcgtgaca
     2521 ttttgcccga gggattcgtc cgggcgttgg gattcgaata ggagaaacac gatggcagtt
     2581 tcaggggaag agaaaatccg cggcctcatc aggctcttac cggtcgcgcg cgctttgaaa
     2641 caggacctgg aaaaagctct cggcatggag gtgttttccg gcgcgaggaa catggcggcg
     2701 aaaagctacg aaggactgcg gcaggctgcg gtggcactca cggacgaccc gtacctgaac
     2761 gcgcttgaac tggaggtctt tccaaccgcg actgacaagg agaagactgc cattgttgcc
     2821 cttgcagcgg gccagctcct tgcatatctg gaagggcaga caggcgtagg cgcggtgtcc
     2881 ggcggaggga attaccactt ctccgtgcaa tcagcaccaa acgtgaacgg ggtgaacctg
     2941 aggaacgtgg attcgaggat cgcagcccgg cttttgggca gcgaaattga ggaagaggac
     3001 tctgaggaag acgacgagaa ggagtgagcg gcgggcagtc gtgaagatca ctccgcacct
     3061 catcggtcgt cgagtgccag ccgcacgcgg aatccacacg accgcgcctt gagcatagct
     3121 ctcgtgtccg atgccgtcat tcccgcgaag gcgggaatcc agcacacgcc ccaggcgtgc
     3181 gcaccgcacc tccccggtca tcgagtggcg gccgcccacg ggcggcagtt gtatcgagaa
     3241 gaccgcgcgg gatgatggga cgcgtcggct aacggattcc ggaaa
//
LOCUS       DAOTNG010000335         3282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23012_length_3282_cov_4.875426, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000335 DAOTNG010000000
VERSION     DAOTNG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23012_length_3282_cov_4.875426"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1038
                     /locus_tag="P5179_12745"
     CDS             181..1038
                     /locus_tag="P5179_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRS96130.1"
                     /translation="MAGTLHGYFERLTGRGFLEDGIIQADEGSVSKDISPILEGWDFR
                     PDRPAVRRIVGDDGRPRLQIRLDLGLLQMEISGRPDGKRPHGFTTALEYYMHLAEEAP
                     GGEHEGGEFRLDEEDCVRLQQESMQFYHRRISWLELGEYRAAEEDADHNLKIIDFVVK
                     YAENDQLREMFTQWKPFILVHKFLAGARQAWEAKDFDGAVERINRGIEDIAEIYREQG
                     REDAIENSGEISYLKKWLEDISQTRPLTLEQRVERELREAIASEHFERAAVLRDRLQA
                     LRQSARTSE"
     gene            1112..1184
                     /locus_tag="P5179_12750"
     tRNA            1112..1184
                     /locus_tag="P5179_12750"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1145..1147,aa:Phe,seq:gaa)
     gene            1279..2403
                     /gene="aroB"
                     /locus_tag="P5179_12755"
     CDS             1279..2403
                     /gene="aroB"
                     /locus_tag="P5179_12755"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRS96131.1"
                     /translation="MARTETVEVPLGSRTYPVVIGSSMLCTLGKSVRRVTAGRLVGVV
                     SDKTVWSLYGDQCSASLEAAGFHVVPKIIEPGEEHKTLHTVAGILDHFLAAGFDRESL
                     VIGFGGGVAGDVAGFVAAICLRGLPFVQVPTTVVAQVDASVGGKTGVDHPLGKNLIGA
                     FHQPSLVFVDTALLASLSERDLRAGLVEVLKHAVIRDPGLFDLLEASLDAFVAGTASA
                     DSWIDLVAANCRIKASVVGQDEKESGLRAILNYGHTAGHAIEKLGAYQRYRHGEAVGF
                     GMMVAGRMACERGLWRSDELFRQRRILRRLLRIPPPTDMAPRDIWNAMKGDKKSLGGV
                     LRFVMPRGIGDATVVSDVTEDEFAAAWAVTVNREEEPHVG"
     gene            2393..3043
                     /locus_tag="P5179_12760"
     CDS             2393..3043
                     /locus_tag="P5179_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS96132.1"
                     /translation="MSDELLDVCDDNGCHVAEATRSEVHEQGLWHKTVHVWVFAPGLG
                     DGHVLFQQRSAAKDIAPGMIDVAVGGHLNKGENPLAGAVRELGEELGLHVPASRLRFL
                     GVRQSAHRFGQFVDREIQTVYGIVDNRGLEDYSVAAEEIAAIVHVPAGDGLELFLGRV
                     NRLVAPVFYSTNPGNAPATGTRVLTNADFLPSVDGYFARVCLAAQHVRRGDADVLL"
     gene            3058..>3282
                     /locus_tag="P5179_12765"
     CDS             3058..>3282
                     /locus_tag="P5179_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRS96133.1"
                     /translation="MRVILDCDTKNEIDDQFAITYAFAKGVRIEAIVNVQNTHSNGPE
                     SVTIYQEEAAAILAALGRPEVPNLRGVERPL"
BASE COUNT          671 a          906 c         1030 g          675 t
ORIGIN      
        1 aacgggaaac tggcgtacga cggcacgcag tttcttacgg agtaagaaga actccggtcg
       61 cggacctgag cttccccgct cgcttcgcga ccggcgaggt gcgcaaccgc acgcggaaac
      121 cccaatagga actctcgctt cgtatatttt gtacgtgcac tccagatgct ggaacccctt
      181 gtggcgggca cactccacgg ttatttcgaa cgcctgacag ggagggggtt tctggaggac
      241 ggtattattc aggctgacga gggttctgtg agcaaagaca tttccccaat cctcgaaggg
      301 tgggacttcc gaccggaccg gccggcggtg agacgcattg tcggcgacga cgggcggcct
      361 cgcctccaga ttcggctgga tctcggcctc ctccagatgg agatttccgg aaggcctgat
      421 ggcaaaagac ctcacggctt caccactgca ctcgagtact acatgcacct tgcggaggaa
      481 gcacctggcg gggaacacga aggtggcgag ttcagattgg atgaggaaga ttgtgtgcgc
      541 ctgcagcagg aaagcatgca gttctaccat cgccgcatca gttggcttga gctgggggag
      601 tatcgcgccg cagaggagga tgcggaccac aatctgaaaa tcatcgattt cgtcgtgaag
      661 tacgccgaaa acgaccagct gcgcgaaatg ttcacgcaat ggaaaccatt cattctcgtc
      721 cacaagtttc tcgcgggcgc gcgccaggcg tgggaagcca aggattttga cggggccgta
      781 gagcggatca atcgcggaat tgaagacatc gctgaaatct accgggaaca gggccgagag
      841 gatgccatcg agaactccgg cgagatcagt tacctcaaga aatggctgga agacatctct
      901 cagacgcggc cattgacgct ggagcaacgg gtggagcgcg aactgcgcga ggcaatcgct
      961 tcggagcatt tcgaacgggc ggccgtattg cgcgaccggt tgcaggccct tcgacagagc
     1021 gctcgcacga gcgaatgagc gtcttcggca ttgaccgatg ccgcggcatt gaccgatgtc
     1081 ggcggacgtc ctatacttaa ttgtctttca tggcgctcta gctcagttgg tagagcaagg
     1141 gactgaaaat ccctgtgtcc ccagttcgat tctggggggc gccacctttt gccgcgcgcc
     1201 ccgatgattc cgacgggaaa tcggggcgct ttgcgttcca cccgccttcc gcgaattaca
     1261 ggtacgaggt gcctttccgt ggcaagaacc gaaaccgtgg aagtgcctct gggcagcaga
     1321 acctatcccg tagtcatcgg gagttccatg ctgtgcacgt tggggaagtc agtaaggagg
     1381 gtgacggcgg ggcgccttgt cggcgttgtt tctgacaaaa cagtatggag cctttacgga
     1441 gaccagtgct cagcgagtct tgaggcggcg ggtttccacg ttgtcccgaa gatcatcgag
     1501 cccggcgagg aacacaaaac gcttcacacg gtcgccggaa tactggatca cttcctggcc
     1561 gcaggcttcg accgcgaaag tctggtgatc ggatttggtg gcggcgttgc gggcgatgtt
     1621 gccgggtttg tcgccgccat ctgcctcagg ggactgccct tcgttcaagt tcccaccacg
     1681 gttgtggctc aggtcgacgc aagcgtcggc ggcaaaacgg gtgtcgatca tccactcggc
     1741 aaaaacctga ttggtgcgtt tcatcaaccg tccttggtgt ttgtcgacac cgccctgctg
     1801 gcctcccttt cggaacgcga cctgcgggcc gggcttgttg aagtcctcaa acacgcagta
     1861 attcgcgatc cgggtttgtt cgatctgctt gaggcgtcgc tggatgcttt tgtggcagga
     1921 accgcttctg ccgattcgtg gatagacctc gtcgccgcaa attgccggat caaggcgtcc
     1981 gttgtcggtc aggacgaaaa ggaatccggc ctccgtgcca tcctcaacta tggacatacc
     2041 gcaggacacg cgattgaaaa gctgggagct taccagcgct atcgccacgg agaagcagtc
     2101 gggtttggaa tgatggtagc cgggcggatg gcgtgcgaac ggggcctgtg gcgctcggac
     2161 gaactctttc ggcagcgaag gattctccgg cgcctgctcc gcattccccc gccaaccgac
     2221 atggcacccc gtgacatctg gaatgccatg aaaggcgaca agaaatccct tggtggggtg
     2281 ctccgtttcg tcatgccccg gggcattggc gacgctacag tcgtctccga cgtaactgag
     2341 gatgaatttg ccgccgcatg ggccgtcacc gtcaaccgcg aagaagaacc ccatgtcgga
     2401 tgagttgctg gacgtatgtg acgacaacgg ttgtcacgtg gccgaggcaa cccgttcgga
     2461 agtccacgaa cagggccttt ggcacaaaac cgtccatgtg tgggtatttg cgcccggtct
     2521 gggagatggc cacgttctgt ttcagcagcg ctccgcggcg aaagacatcg ctcccggcat
     2581 gatagacgtt gccgtcggag gccatctcaa caaaggggag aatcctctgg cgggagcggt
     2641 ccgagaactg ggggaagaac ttggcctcca cgttccggct tcccggttac gtttcctcgg
     2701 cgtgcgccag agtgcgcacc gcttcgggca gttcgtggac cgggagattc aaacggtgta
     2761 cggcattgtc gacaaccgcg gtctcgaaga ctactcggtt gcggcggagg aaattgcagc
     2821 gatcgtccac gtcccggcgg gtgacgggtt ggagcttttt ctgggccgcg tgaaccggtt
     2881 ggttgctcca gttttctact caacgaatcc gggcaatgcg ccagcaaccg gtactcgggt
     2941 tctgacaaat gcagatttcc tgccgtctgt tgacggctat ttcgcgcggg tgtgccttgc
     3001 ggcacaacac gttagacgcg gcgatgctga cgtgcttctg taaaaggcag cgtgacaatg
     3061 cgtgtgatcc ttgattgcga cacgaaaaac gaaattgacg atcagttcgc gatcacgtat
     3121 gcgttcgcga aaggtgtgcg gattgaggcg atcgtcaatg ttcagaatac gcattcaaac
     3181 ggacccgaat cggtaacgat ctaccaggaa gaagcggcgg ccattctggc ggctctcggg
     3241 cggcctgagg tcccgaatct gcggggtgtg gagcgtcccc tc
//
LOCUS       DAOTNG010000336         3277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23060_length_3277_cov_5.357852, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000336 DAOTNG010000000
VERSION     DAOTNG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23060_length_3277_cov_5.357852"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="P5179_12770"
     CDS             complement(<1..1005)
                     /locus_tag="P5179_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRS96134.1"
                     /translation="MALRVVFAAEHVADFADVLDEIRGRGYEVQLIHPFDYQPTASRS
                     SDQDAVAIFIPLDRGGVSRLGGYQSTDVSLPRSAATFLIGMAPETTSDGTQSHQRRAL
                     DGVIPYPPSLEDLLRVLSGCEARLVAGTPDSGSPPDNETDHSRLKEFYERIVDTISDG
                     IRVVDGRDRTIVLANRAHLAWIGKQAHEVIGVRARDVPWGFDANDCPVQMTDIVDAAI
                     ETAEPARRLIATCPHAGRELRWIDVVAYPMSGLSGSVDHVVLVGRDVSERVRMETSLA
                     AERDRRDHVLRSAPVGILTTDSTGTIEFANPPIAQIMGLEGTEAVVGKNILMLPQLQA
                     M"
     gene            complement(1059..>3277)
                     /gene="gyrA"
                     /locus_tag="P5179_12775"
     CDS             complement(1059..>3277)
                     /gene="gyrA"
                     /locus_tag="P5179_12775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS96135.1"
                     /translation="SVATELLADLDRDTVDFQSNYDERLQEPKVLPARFPALLCNGAS
                     GIAVGMATNIPPHNVGEVVDALIAQIENPDIDVNGLMQHIKGPDFPTGAIIYGIEGIR
                     EAYATGRGLIKVRARANIETLKNDRQNIVITEIPFQLNATTLKEAIASVARNRVVDGI
                     ADFRDESDRDGMRMVIELKRDAYAEVTLNQLFKHTPLEQTFGINLLALVNGQPKLLTL
                     KEMLQHYIDHRREVVRRRTSFDLNQAQAQAHIVEGLLLALRDIDNIIETIRKSRDAAE
                     ARGQLMSVFQLSEKQADAILDMNLRRLTSLEVSKLENQYADLTTQIAEFQTILSSDAN
                     ILAVVKKELLDVRKKHGDERRTEIVASASDFSIEDLIAEEDMVVTISHTGYIKRIPIN
                     TYRQQRRGGRGVTGMTTKEEDFVEHLFVASTHSYLLCLTEKGRCYRLRVHEIPQGSRS
                     SKGRPLVNVLEAAKDEIIAAVVPVRHFEEGRYLVMVTAKGTIKKTPLSAFANINRLGV
                     IAINVLEEDELIEAAVTDGTYDIILGTRNGYAIRFPEDTVRPMGRTAAGVRGIRLRGD
                     DRVVGMVVNKHHDRTLLTVCENGHGKRSPIEDYRITNRGGKGIINIKATERNGAVVAI
                     KEVIDEDELMIVTQNGILIRLPIADLSVIGRNTQGVRLIRIEEGDVVQDVARVVVKNG
                     DAGAASPEGEAEEEVEEDSEAPANGLPEEEYRDDAESREDEEDDGGDHVDLADEEE"
BASE COUNT          615 a          984 c          912 g          766 t
ORIGIN      
        1 cattgcctgc agttgcggta acatgaggat gtttttccct acaactgctt cagtgccctc
       61 caaccccatg atttgggcaa tcggtggatt tgcgaattcg atcgtacccg ttgaatcggt
      121 ggtcaggatg ccgacagggg ccgagcggag gacgtggtcg cgcctgtctc tttcggccgc
      181 aaggctggtt tccattctca cacgttctga gacgtcccta cctaccaaaa cgacgtgatc
      241 gacggaaccc gaaaggcccg acatcgggta ggcgactaca tcaatccagc ggagctcacg
      301 tcccgcgtga gggcacgttg cgatcaatcg cctcgccggc tcagcggttt cgatcgcagc
      361 atcaacgata tccgtcatct ggacagggca atcgtttgcg tcgaaacccc acggaacgtc
      421 ccgcgcacgg acaccgatca cctcgtgcgc ctgcttccct atccacgcga ggtgtgctct
      481 gtttgccagg acgatcgtcc gatcccttcc gtcgacaacc cggataccat cgctgattgt
      541 gtccacgatg cgctcgtaga attccttcag tcggctgtga tccgtttcat tgtcgggtgg
      601 tgagccggag tcgggtgtac ccgcaaccag ccgggcttcg catccggaaa gcacgcggag
      661 cagatcctcc aacgaggggg gataggggat caccccatca agcgcccgtc tctgatgact
      721 ttgtgtgcca tcagaggtgg tttccggggc catgccgatc agaaaggtgg cagcgctgcg
      781 cggaagactg acatcagtcg actgatatcc tccgagtcga gacaccccgc cccgatccag
      841 cggaatgaaa atggcaacag catcctggtc gctcgaccgg gatgctgttg gttggtaatc
      901 gaaggggtgg atcagttgga cttcataccc gcggccacgg atttcgtcca gaacgtcggc
      961 gaagtccgca acgtgttctg cggcgaaaac aacacgaagt gccatcgctg tccatcgcag
     1021 aaacaggtaa ggttttcacg cgtggaaccc gcctaaagct actcctcttc gtctgcgagg
     1081 tcaacgtgat cgccaccgtc gtcctcctca tcttcacgcg attcggcgtc gtccctgtat
     1141 tcctcttcag ggaggccatt cgctggggct tctgagtcct cttccacctc ttcttccgct
     1201 tcaccctcag gagatgctgc gcccgcgtcg ccgttcttta ccacaacgcg cgccacatcc
     1261 tgaacaacat caccctcctc gattctgatc agacgaacac cctgcgtgtt ccgtccaata
     1321 accgacaagt cggcgattgg gaggcggatc agaatcccgt tctgggtgac aatcatcaac
     1381 tcgtcctcgt ctatcacttc cttgatagcc acaaccgcgc cgttccgttc cgttgccttg
     1441 atgttgatga tgcccttacc gccacggttt gtgattcgat agtcctcgat gggggagcgc
     1501 ttcccgtgcc cgttttcgca cacagtcaac agagtgcgat catggtgctt gttcaccacc
     1561 ataccgacca ctctgtcgtc accccggagt cggatgcccc gcactccagc ggcggttctg
     1621 cccatggggc gaactgtgtc ctccgggaac cggatggcgt acccattcct ggtgccgaga
     1681 atgatgtcgt acgtcccgtc cgttacggcc gcttcgatca gttcgtcttc ttccagcacg
     1741 ttgatcgcga taacgccgag gcgattgata tttgcgaacg cggagagtgg tgtcttcttg
     1801 attgtccctt tcgccgttac catcacgagg tagcggcctt cttcgaaatg ccggaccggc
     1861 accactgccg cgatgatttc gtcctttgca gcctcgagaa cgttgacaag cggccttcct
     1921 ttgctggaac ggctcccctg cggtatctca tgcactcgaa ggcggtagca gcgtcctttc
     1981 tccgtgagac agaggaggta actgtgtgtc gacgcgacaa acagatgctc gacaaaatcc
     2041 tcctctttcg tcgtcatacc agtcacgcca cggcctcccc gacgctgttg ccggtaggtg
     2101 ttgatcggta tccgtttgat gtatccggta tggctgatcg tgactaccat gtcttcttcg
     2161 gcaatcagat cttcgatgga aaaatcggat gccgaagcca cgatctccgt gcgtcgctcg
     2221 tcgccgtgct tcttgcgaac atcgagcagc tcttttttca cgacggcaag gatattcgcg
     2281 tctgaactca ggatggtctg gaattcggcg atctgtgtgg tgaggtcggc gtactgattc
     2341 tccagtttgc tcacttccag actggtgagg cgccgaaggt tcatatcgag gatcgcgtcg
     2401 gcctgcttct cggaaagctg gaagacgctc atcaactgcc cgcgggcctc ggcggcatca
     2461 cgcgattttc ggatggtctc gatgatattg tcaatatccc gcagcgccag aagcagtccc
     2521 tcaacgatgt gcgcctgcgc ctgcgcctga ttgaggtcga agctggtccg ccttcggacg
     2581 acctcgcgac gatggtcaat gtagtgctgc aacatctcct tcagtgtcag gagcttcggt
     2641 tgcccgttga ccagcgcgag gaggttgatt ccgaacgtct gttccagtgg cgtgtgcttg
     2701 aagagctggt tcagcgtaac ctctgcgtac gcatcacgct tgagctcgat taccatgcgc
     2761 atgccatcgc ggtcggactc atctcggaaa tcggcgattc cgtctacaac tcgattccga
     2821 gcgacggagg cgatggcctc tttcagagtt gtagcattga gctggaacgg tatctccgta
     2881 atgacgatgt tctgtcgatc gtttttgaga gtctcgatgt tggcgcgtgc ccggactttg
     2941 atcaggcctc gtccggtggc ataagcctcc cgaatccctt cgataccgta aatgatcgcc
     3001 ccggtgggga aatccggtcc cttgatgtgc tgcatcaggc cgtttacgtc aatatccggg
     3061 ttctcgatct gcgcgatcag cgcatctacc acttcgccca cgttgtgggg cggaatgttg
     3121 gtggccatgc ccacggcgat gccggaggcg ccattgcaca acagggccgg gaaccgagcg
     3181 ggaagaacct tcggctcctg aagccgttcg tcgtagttgg actggaagtc tacggtgtcc
     3241 cgatccagat ccgcgagcag ttccgtggcg acggacg
//
LOCUS       DAOTNG010000337         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23300_length_3249_cov_4.432373, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000337 DAOTNG010000000
VERSION     DAOTNG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23300_length_3249_cov_4.432373"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="P5179_12780"
     CDS             <1..1010
                     /locus_tag="P5179_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96136.1"
                     /translation="WNRSHVDRATILVRAPRPTTMQPPPPPKDVRQFWLDPDYRIAAA
                     EYQMASTYCGGEAFPWFDTNIGPGSLALFLGSDAILHPTTVWYTPIADRPGDVPPIRL
                     DTSCEWWQKSVRLVREGMIRGEGRFLSSVPDLIENLDIAASLIGGQQLLYALVDDPGG
                     VKRMVREINAAYFQCFDLMYEMLDGDRLGCCFCAFQIWGPGKTAKLQCDFSDMISPEM
                     FEEFVLPGLIEQTEYLDYTIYHLDGPRAIRHLDLLLSIPRLNAIQWTPGDGNPEVGDE
                     TWWPMYRKILAAGKGVHLGTAYRDVEPLVKHLGNDGVYVATHAPSVEAADDLLRRAKT
                     W"
     gene            complement(1053..1128)
                     /locus_tag="P5179_12785"
     tRNA            complement(1053..1128)
                     /locus_tag="P5179_12785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1093..1095),aa:Gly,seq:gcc)
     gene            1191..1940
                     /locus_tag="P5179_12790"
     CDS             1191..1940
                     /locus_tag="P5179_12790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRS96137.1"
                     /translation="MSSYLVKPADICGDTAVLSGDEAAHASKSARKRPGDELVLIDGV
                     GTGYRCVVRTVEGDRVVCQIREVIPHMGEPRTHVTLAVGMVKGDRFDSLVETAVELGA
                     GAIVPLETRFSIVHHTSENRQRRWNRIAEAAAKQCLRSRIPSVEKPQPLRSFAERTGE
                     FDVVFVAWEGQQESANPIPVAPLVGSILLVVGPEGGFHDEEISLLRSKGAHTVPLGPR
                     RLRTGTAAIALLTLALQGVGEYARCLAPHVD"
     gene            2032..2322
                     /locus_tag="P5179_12795"
     CDS             2032..2322
                     /locus_tag="P5179_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96138.1"
                     /translation="MGEVRVTPERIHGFGCDGNGSSWIIGPDGVILKEASVFGEEAVV
                     ADVAIADATRRLALLSRELAPLGKWWNLGVKILGDLSSGEWTSPEIPGTPKK"
     gene            2330..3148
                     /locus_tag="P5179_12800"
     CDS             2330..3148
                     /locus_tag="P5179_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS96139.1"
                     /translation="MLKVRFAAAPILWRGVPFEGIAREVADIGFDGIEAPVTRYREDR
                     ETLRRLLGETGLALASTYTSGYYMDPGSREDEIRAVVEIAKLLPEFGCHTMISAPQGL
                     RKERKAYSLWEYRTLFEALNEIGRRIADFGVTQVYHNHAWTVLETRLEVDLLCAHTDP
                     RYVKMGFDTGHLHLGWSDPVELIRLYGDRVAYLHFKDARGRADQWSLMGKDDDVWCEL
                     GAGEVDLAGIVRELNARNWEGWLSYEQDKTAKTPRESASESLRYAKKLVAEFGS"
BASE COUNT          685 a          905 c         1026 g          633 t
ORIGIN      
        1 ggtggaacag aagccatgtc gacagggcca cgattctcgt gcgtgctcct cggccgacca
       61 cgatgcagcc gccacctccg ccgaaggatg tccggcagtt ctggctcgat cctgattacc
      121 gcattgcggc ggcggaatat cagatggcgt ctacgtactg cggtggagaa gcgttcccct
      181 ggtttgatac gaatatcggc ccgggatctc tcgcgttgtt ccttgggtca gacgcgattc
      241 tccatccgac taccgtgtgg tacaccccga ttgcagacag gccgggcgat gtaccgccaa
      301 tcaggcttga tacgtcgtgc gaatggtggc aaaaaagcgt cagactggtc agagagggca
      361 tgattcgcgg ggaaggcaga ttcctgtcgt ccgttcccga ccttatagag aacctcgaca
      421 tcgctgcttc gctgattggt ggccagcagt tgctgtacgc actggtggat gacccgggtg
      481 gggtcaagcg aatggtccgc gaaatcaacg cggcgtactt ccagtgtttt gatctgatgt
      541 acgaaatgct ggacggtgac cgccttggtt gttgtttctg cgccttccag atctggggcc
      601 ccggcaagac cgccaagctc cagtgcgatt tctctgacat gatttcgccg gaaatgttcg
      661 aggaattcgt gctcccggga ttgatcgaac aaacagagta cctggactac acgatttacc
      721 accttgacgg tccccgggcc atccgccacc ttgacctctt gctttcaatt ccgcgactga
      781 acgccatcca gtggactccc ggcgacggga acccggaagt gggagatgag acgtggtggc
      841 cgatgtacag gaagatcctt gcggcgggaa agggcgttca cctggggacg gcatacaggg
      901 acgtggagcc gctggtgaag cacctcggca acgacggagt gtacgtggct actcacgcgc
      961 cttccgtgga agccgctgac gaccttctga ggcgcgcgaa gacctggtaa aaaaagagag
     1021 cggctcgctg gcgcagcgaa ccgctcaaaa aatggagcga cggacgggat tcgaacccgc
     1081 gaccccaacc ttggcaaggt tgtgctctac cagctgagct accatcgcgt ggattagaga
     1141 gtatagtgaa cccggttgaa cgagtcaagg tttcaaacta cggtgcttcc gtgtcatcct
     1201 acctggtaaa acccgccgac atttgtggcg acacggccgt actctccggg gatgaagcag
     1261 cacacgcctc gaagtccgcg cgcaagcggc ccggtgatga actcgtcctg attgacgggg
     1321 tgggaaccgg gtaccgttgc gtcgtgcgca cggtggaagg cgaccgcgtc gtctgccaga
     1381 tacgcgaggt gatcccgcat atgggcgagc cccggaccca cgtcactctc gctgttggca
     1441 tggtgaaagg cgaccgcttc gatagcctcg tcgaaaccgc ggtggaactg ggcgcaggtg
     1501 ccatagtacc acttgagact cggttctcaa ttgtgcatca cacctccgaa aatcgtcagc
     1561 gccgctggaa caggattgcg gaagccgccg caaagcagtg cctccggtcg cgaattcctt
     1621 cagtggaaaa accccagcct ctgcgctcgt ttgcggagcg aaccggcgaa ttcgacgttg
     1681 tttttgtggc gtgggagggt caacaggaat cagcgaatcc cattccggta gcaccgttag
     1741 tcggaagcat cctccttgtg gtagggcctg aggggggatt tcacgatgaa gaaatcagcc
     1801 tgctccgcag caaaggggcc cacaccgtgc cgctcggccc acgtcggctt agaaccggca
     1861 ccgcggcaat tgccctgttg accctggcgt tgcagggagt tggcgaatac gcacggtgcc
     1921 ttgccccgca cgttgattga tcctctttcg gagattctcc ggtgaaagaa cccccgagca
     1981 tcctgcgtac tgctgtgcag tccaaatcat gtgatctgag cgaacagccg catgggggag
     2041 gtccgggtaa cgccagagag aattcatgga ttcggttgtg acgggaacgg cagcagctgg
     2101 atcataggcc cggacggagt gatcctgaaa gaagcgagtg tcttcggcga ggaagcagtt
     2161 gtcgcggacg ttgccatcgc agacgccaca cggcgcctag ctcttctgag cagggaattg
     2221 gccccgctcg gcaaatggtg gaatctcgga gtgaaaatcc tcggcgacct ctcctctgga
     2281 gagtggacgt cgccagaaat tccaggcaca ccaaagaaat gagggacgca tgttgaaggt
     2341 tcgttttgcc gccgcgccca tcctgtggag gggcgtaccg ttcgaaggga ttgcacgcga
     2401 ggtagctgac atcgggtttg acgggatcga ggctcccgtc acgcgctaca gggaagacag
     2461 ggaaacgctt cgccgcctgc tgggcgaaac cgggctcgcg ctcgcgagca cgtatacgtc
     2521 cggatactac atggacccgg gctcaaggga ggatgaaatt cgcgcggtgg tggagattgc
     2581 gaagttgctg ccggagttcg gatgccatac gatgatcagt gcaccgcagg ggttgcggaa
     2641 ggagcgtaag gcatactccc tctgggagta tcggacgctc ttcgaagccc tgaacgaaat
     2701 cggtagaaga atcgctgact tcggcgttac gcaggtgtac cacaaccacg cttggacggt
     2761 ccttgagacg cgccttgaag ttgaccttct gtgcgcacat accgacccac gatacgtgaa
     2821 aatgggattt gacacgggtc accttcacct ggggtggtcg gaccccgtgg aacttatccg
     2881 tctgtacggt gatcgcgtgg cctacctgca tttcaaagac gctcgcggga gagcggacca
     2941 gtggtcgctc atgggcaaag atgacgacgt gtggtgcgaa ctcggtgccg gtgaggtgga
     3001 cctcgccggg atcgttcgcg aactgaacgc ccgcaactgg gaaggctggc tcagctacga
     3061 acaggacaag accgcgaaga cgccccggga atctgcttcg gagagcctga ggtacgcaaa
     3121 aaagctcgtc gcggaatttg gcagttgagg ggccgcgacc cgcaacacac aggaggccgg
     3181 acgcgatgct gaccggcctc ctgttttttg ctgtgggtga gatactccga cgacgttcaa
     3241 acccgcacg
//
LOCUS       DAOTNG010000338         3231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23438_length_3231_cov_5.982368, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000338 DAOTNG010000000
VERSION     DAOTNG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23438_length_3231_cov_5.982368"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="P5179_12805"
     CDS             <1..1086
                     /locus_tag="P5179_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRS96140.1"
                     /translation="PSATEAIELLKKAGVKVKVLTGDNGLVTEKVCRNVELEVDRIVT
                     GTELSQLSPVDFLETVQNNNVFVKLSPAQKEQIVKELRKNGNVVGFMGDGINDAPALK
                     AADVGISVDSAVDVAKESADIVLLEKSLLVLEEGIMEGRRVFANIVKYIRMGASSNFG
                     NMFSVLGASYLLPFLPMQPVQILTNNLLYDFSQTGIPADNVDEELLAKPLKWNISNVK
                     RFMMFVGPISSIFDYATFALMWFFFRSSEYLNPSLGGAEKSALASLFQTGWFVESLLT
                     QTLIVHIIRTRRIPFFGSRASLHMTLTTLAVVAVGAWLPYSPFAPGLGMTPLPAVYWA
                     WIAAFLVSYGILTHLVKTWFFNRYGGD"
     gene            1089..1892
                     /locus_tag="P5179_12810"
     CDS             1089..1892
                     /locus_tag="P5179_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRS96141.1"
                     /translation="MDTLLDALQEGRLFELPENDKSHALQFLAHIIEAFPETPAGTDV
                     VETVMKREQVATTAIGKGWACPHARIPGDEDLMCVVGWSPAGIDYGSPDGAPVSLVVM
                     YLVPDNQRNHYLREISSLAKAIESYSGGEKLREAADLDDVRGYLLDLIETGEKTVVPD
                     TRARMIRLQTKPAREAAAVQDLSNLIVEPVTIVAGPGMNHVVLAQNPVLFEQLQSVSG
                     LAERLDAEGLFQNGGWRVLKRSSTLYQGGRVAHDCLAIRMAPQTPARTA"
     gene            2051..>3231
                     /locus_tag="P5179_12815"
     CDS             2051..>3231
                     /locus_tag="P5179_12815"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS96142.1"
                     /translation="MQPAAPNSPNRPYSHASFRGGTIPGVCWRSGSAVFDEMFRDGRL
                     VCRYWNANGQIWPEMHFENTAWAEDQPADTFHLAINRHDLAGGYSWLGAEATPDPSAY
                     RQGVSGSDELPVTHGVIRLAHAEAGVEVAVHTRLDGGPFIIRWLEITNLSSASVAISS
                     VSPFAGALWAHRYEEHLPAGLSSPFEVAYNHAFEWGHEGDFWFQPLMPGVFAVDGGKK
                     GRSGWGRPAFWARNLCNGQTFVCELAWGGNYEFALDCRFHPRTAQDPTRLDTPRAELY
                     FRMGLSGYDEALRVVEPGETVTTPAVHAALFHADTDDIVQVTHEHVRHVVMPAQIPGR
                     EVEIEANHRGYLCDRENVPDILKDVDVAAAIGTEMYVIDAGWYGNEPNQWGNNVGDWH
                     DG"
BASE COUNT          642 a          972 c          992 g          625 t
ORIGIN      
        1 ccctcagcga ctgaggcgat tgagctgttg aaaaaggcgg gtgtcaaggt gaaagttctg
       61 accggcgata acggccttgt caccgaaaag gtctgccgca acgtggagct ggaagttgac
      121 agaatcgtca ccggcacaga gctttcgcag ctttcgcccg ttgattttct ggagaccgtg
      181 cagaacaaca acgtcttcgt caagttgtct ccggcgcaga aagagcagat cgtgaaggag
      241 ctgcggaaaa acgggaacgt tgtcggtttc atgggagacg gtatcaacga tgcgccggcc
      301 ctgaaggcgg cagacgtcgg catttcggta gattccgcgg tggacgtggc caaggaatct
      361 gcggatatcg tgctgctcga gaagagcctt ctcgtgctgg aagagggcat catggagggg
      421 cggcgggttt tcgccaacat cgtcaaatac atccgcatgg gcgccagctc caactttggc
      481 aacatgttca gcgtgctcgg ggcgagttac ctgttgccgt tcctgccgat gcagccggta
      541 cagattctca cgaacaacct gctgtacgac ttctcgcaga ccggcatccc tgccgacaac
      601 gtggacgagg aactgctcgc caaacctctc aaatggaata tctcgaatgt caagcgattc
      661 atgatgttcg tggggccgat cagctccatc ttcgattacg ccacgtttgc gctgatgtgg
      721 tttttcttca ggtccagtga atacctgaat ccctccctgg gaggagccga gaagagtgct
      781 ctcgcaagcc tcttccagac gggatggttc gttgagtcgc tcctcacgca gacactgatc
      841 gtccatatca tccggacgag aagaatcccc tttttcggaa gtcgagcctc cctccacatg
      901 acgttgacca cgcttgctgt ggtggctgtc ggggcgtggc tgccatactc tcccttcgcc
      961 cccggtctgg gaatgactcc actgcccgcg gtgtactggg cgtggattgc cgcgtttctt
     1021 gtgtcctacg ggattctgac gcatttggtg aagacatggt tcttcaaccg atatggaggt
     1081 gattgacgat ggatacactc ctggatgcgc tgcaggaagg tcggctgttc gagctgcctg
     1141 aaaacgacaa gtcgcacgcg ttgcagtttc tggcacacat catcgaagcg tttccggaga
     1201 cacccgccgg gactgacgtg gtggaaaccg taatgaaaag agagcaggtt gccaccacag
     1261 ccataggcaa aggctgggcc tgccctcatg cgcgaattcc cggggacgag gaccttatgt
     1321 gcgtggtggg atggagcccc gccggaattg attacggctc gccggacgga gcgcccgttt
     1381 cgctcgtcgt catgtacctc gttccggata accagagaaa ccactacctg cgcgagattt
     1441 cctcgctggc aaaggccatc gaaagctatt ccggcggaga aaaactgcgc gaagccgcag
     1501 accttgacga cgtccgcggg tatctgctcg atctcatcga aacaggcgag aagacagtcg
     1561 tcccggacac gcgtgcacgg atgatccgtc tgcagaccaa accggcacgg gaagcggccg
     1621 ccgttcagga cctttcaaac ctgatcgtgg aacccgtaac gatcgttgcc ggcccgggaa
     1681 tgaaccacgt tgttctggca cagaatcccg ttcttttcga acaactccag tccgtgagcg
     1741 ggcttgccga acggctggac gcggaaggcc tgtttcagaa tggcgggtgg cgcgtgttga
     1801 agcggagttc caccctttat caggggggac gcgtcgcgca tgactgcctg gcaataagga
     1861 tggctcccca gaccccggcg cgcacggcgt agagccgcgt tccctttcga gcccctgcaa
     1921 acgccgcagc accttgccgg ctggaaaggc cccatacatc ccccaccacg tttgtccccg
     1981 aagcgcgccg cgctacactt tgcacagtgc ctgagataac cacgcgcgtc ccgcttcccg
     2041 aggcaacccc atgcaacccg cggccccgaa ctctcccaat cggccctatt cccatgcttc
     2101 ctttcgcggc ggaaccattc ccggtgtgtg ctggcgctcc ggcagcgcgg tgttcgacga
     2161 gatgttccgc gacggccggc tggtgtgccg gtactggaac gcgaacgggc agatatggcc
     2221 tgaaatgcac ttcgaaaaca ccgcatgggc ggaagatcaa cccgctgaca cgttccacct
     2281 cgccatcaac cggcacgacc tcgccggcgg gtactcatgg ctgggcgcgg aggccacacc
     2341 cgacccatcg gcgtaccggc agggggtgag cggcagtgac gaactcccgg tgacgcacgg
     2401 cgtcatccgg ctcgcccacg ccgaggcggg agtagaagtt gccgtgcaca cccgtctcga
     2461 cggcggcccg ttcatcatcc gctggctgga aatcaccaac ctcagttccg cttctgttgc
     2521 tatttcttcg gtatctccct tcgccggagc gctctgggcg catcggtatg aagagcacct
     2581 gcccgctggc ctttccagcc cgttcgaggt tgcgtacaac cacgccttcg agtggggcca
     2641 cgaaggtgat ttctggttcc aaccgctcat gccgggcgtg ttcgccgttg atggcggcaa
     2701 gaaaggccgc agcggctggg gcagaccggc cttctgggcg cgtaacctgt gcaacgggca
     2761 gacgttcgta tgcgaacttg cgtggggcgg caactacgag ttcgcgctgg attgccggtt
     2821 tcacccgcgc accgcgcagg atcccacccg gctcgacacg ccacgggcgg aactgtactt
     2881 ccggatgggg ctttccgggt atgacgaagc actccgcgtc gttgaaccgg gagaaaccgt
     2941 gacgacgccg gccgtgcacg cggcgctgtt ccacgcggat accgacgaca tcgtgcaggt
     3001 cacgcatgaa cacgtgcgcc atgttgtcat gccggcccag atccccgggc gggaggtgga
     3061 aatcgaggcg aaccaccgcg gctatctgtg cgaccgggag aacgttccgg atatcctgaa
     3121 ggacgtggat gtggcggcgg cgatcggcac cgagatgtat gtgatagacg ccggctggta
     3181 cggcaacgag ccgaaccagt ggggaaacaa cgtcggggac tggcatgacg g
//
LOCUS       DAOTNG010000339         3228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23470_length_3228_cov_4.855027, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000339 DAOTNG010000000
VERSION     DAOTNG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23470_length_3228_cov_4.855027"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="P5179_12820"
     CDS             complement(<1..823)
                     /locus_tag="P5179_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011770893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRS96143.1"
                     /translation="MAEDTLTVTDNRTGKVYVIPIRYGTYPEYGASISAMDLRQIKVS
                     EDDFGLLSFDPALTNTETTASAITFIDGERGILRYRGYPIEQLAEKSTFLEVAYLLIH
                     GKLPTFAEFADWTNKIVKNMLIHESIKKFLDGFHSNAHAMGVFVGTLGAKSTFYPAAK
                     HVLDPKQRMRETINIIASVPTIAAYTYRHSCGLPYVYPDPSLSYIYNFLNMLFRMQPG
                     KEEIYQNPVIERALDVLFILHADHEQNCSTTTMRTVGSALSDPYSAISAAAGALYG"
     gene            complement(884..1708)
                     /locus_tag="P5179_12825"
     CDS             complement(884..1708)
                     /locus_tag="P5179_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRS96144.1"
                     /translation="MKKLELGLMVGFASDPNESFRQVAELGIPTCQVTGTAEAALAGK
                     YSDPEKVRNAADKAGVRISSVFLLWEGQQFDNVNGPSTMGLVAPRFRDERLRSGKLYS
                     DWVARMGVDSITCHIGFIPDDERDPVYETFVDAMRDLALHCKRNGQKFCFETGQELPS
                     TLKRTIRDVGTGNLFVNLDPANLILYGKANPLDAEEIFGEYVRGLHAKDGVWPNRDEA
                     LGHETPLGEGAVRFDLLIRRLKAKGFEGPLTIEREIHGPQQRVDILRAIKILEPML"
     gene            complement(1750..2148)
                     /gene="queF"
                     /locus_tag="P5179_12830"
     CDS             complement(1750..2148)
                     /gene="queF"
                     /locus_tag="P5179_12830"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRS96145.1"
                     /translation="MTTTTGYDENHAKSQGETPAIDVWPNQYRDRSYTIRITIPEFTS
                     MCPKTGQPDFATITIVYEPDALCVELKSLKLYMQAYRNMGIFFENVANKILNEFVAAC
                     KPRRAVVVADFNPRGGIRTSVRVSYSNKSN"
     gene            complement(2164..3039)
                     /locus_tag="P5179_12835"
     CDS             complement(2164..3039)
                     /locus_tag="P5179_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRS96146.1"
                     /translation="MRVILDCDTKNEIDDQFAITYAFAKDVRIEAIVNVQNTHANGPE
                     SVTIYQEETAAILAALGRPEVPNLRGVERPLGNNLSPGSAEGVDFIIDRALTEDPEPL
                     FVIGTGPATDLASALLRAPEIASKARFVWLGGFRDEEEFRRRGSEWNALGDPWAVKAL
                     FESRAAMLSTLFHVPAWGVTEKLVWRTERLVGQLREMGKPIHTLLADRTVEWTTRSGR
                     SLSDNPPKEFWDIGAVDVAIHPERHSLRFMPAHGLGENAEWVFPASGTHVIGIDVDLE
                     TDAILSDALCAFAGL"
BASE COUNT          675 a          966 c          863 g          724 t
ORIGIN      
        1 gcccgtaaag cgcgcccgcc gccgccgaaa tcgcggaata ggggtctgaa agtgcgcttc
       61 ctaccgttcg catcgtggtg gtgctacaat tctgttcatg gtccgcatgc aggatgaaca
      121 gcacgtccag cgcccgttct atcaccgggt tttggtaaat ttcctcctta cccggctgca
      181 tgcgaaaaag catgttcaag aagttgtaga tgtaagagag cgacggatcg gggtacacgt
      241 aaggaagccc gcagctgtgg cggtacgtat aggcggcgat cgtcggaacc gatgcgatga
      301 tgttgatcgt ttcgcgcatt cgctgtttcg ggtccagaac atgtttcgcg gccgggtaga
      361 acgttgattt cgcgccgagt gttcccacaa aaacacccat cgcgtgggca ttgctgtgga
      421 acccgtcgag gaacttcttg attgattcgt ggataagcat gttcttgacg attttgttcg
      481 tccagtccgc aaactcggcg aacgtcggca gtttcccgtg tatcagcagg tacgccactt
      541 ccaggaacgt cgatttctca gcgagttgct caatcggata gccgcggtat cgcaggatgc
      601 cccgctcgcc atcaatgaac gtgatcgcac tggcggtcgt ttccgtgttg gtgagcgccg
      661 ggtcgaatga gagcaatccg aaatcatcct cactgacctt gatctggcgc agatccatgg
      721 cgctgattga cgcgccatat tcggggtacg tcccgtacct gatggggatc acataaacct
      781 tgccggtcct gttgtcagtt acggtgagag tatcctccgc catcttttcg aacctttctt
      841 ccccagtttt cgtgaacaac caccattttc gcgacgcgtg cgatcaaagc atcggttcca
      901 ggatcttgat cgcacgcagt atgtcaactc gttgctgagg cccgtggatc tcccgttcga
      961 tggtcaaggg cccttcaaat cctttggcct tcagtcggcg tatcaacaga tcaaaccgga
     1021 cagccccttc gccgaggggc gtctcgtgac caagcgcctc atcgcggttc ggccacacac
     1081 cgtccttggc atgcagtcct cgcacgtact caccgaaaat ctcctcggcg tccagagggt
     1141 ttgctttgcc gtaaagaatc aggttcgccg ggtcgagatt cacgaacaga ttccccgtac
     1201 cgacgtcgcg gattgttcgc ttgagcgtcg acggcaactc ctgtccggtc tcgaagcaga
     1261 acttctgacc gttccgtttg cagtgaagcg ccagatcgcg catcgcgtcg acgaatgtct
     1321 cgtataccgg gtcgcgctcg tcatccggga tgaacccgat gtggcaggtg atgctgtcga
     1381 cacccatgcg cgctacccag tcgctgtaaa gttttcccga tctgaggcgc tcatctcgaa
     1441 agcgaggtgc aaccagcccc attgtggacg gaccgttgac gttgtcaaat tgctggcctt
     1501 cccagagcag aaacacagaa ctgatccgta cacccgcctt gtccgcagcg ttgcgaacct
     1561 tctccgggtc ggaatacttc ccggcgagcg ctgcctccgc ggttcctgta acctggcatg
     1621 tcggaatacc cagctcagcg acctgtctga acgactcgtt cggatcgctc gcgaagccca
     1681 ccatgagccc cagttccagc tttttcacag cgccatcctt gtgaaaaggt ccacggaaga
     1741 gaagagacgt cagttcgatt tgttcgagta ggaaacgcgt accgaagtgc gaatgccgcc
     1801 ccgcgggttg aaatcagcca caacaaccgc gcgccgcggc ttgcaggcgg ctacaaactc
     1861 gttcagtatc ttgttcgcga cgttctcaaa aaagataccc atattccgat acgcctgcat
     1921 atacagcttc agggacttca gctcaacgca aagagcatcg ggttcataca cgatggtgat
     1981 cgtggcgaaa tccggttgcc cggttttcgg acacatcgaa gtgaactcgg gaatcgtaat
     2041 cctgattgtg taactgcggt cgcgatattg atttggccac acatcaatag caggtgtctc
     2101 gccctgtgat ttcgcatggt tttcgtcgta ccccgtagtt gtcgtcactg tccgcttccc
     2161 tcctcacagg ccggcgaatg cgcacaacgc atcgctcaaa atggcgtccg tttccaggtc
     2221 aacatcgata ccgatcacat gcgttccact ggcaggaaaa acccattctg cgttctcgcc
     2281 aagcccgtgc gcgggcatga aacgcagcga atgacgctcc gggtgtatgg ctacgtccac
     2341 cgcaccgatg tcccagaatt cctttggcgg attatctgag agggacctgc ccgatctggt
     2401 ggtccattcc acggttcgat ccgccagcaa cgtatgtatc ggttttccca tctcccggag
     2461 ctgccccacc agccgctcag ttcgccacac gagcttctca gttacgcccc aggcgggaac
     2521 atggaatagc gtggataaca ttgcggcacg gctttcgaaa agcgccttca cggcccaggg
     2581 gtcccccagt gcattccatt ctgatccgcg tcggcgaaat tcctcctcgt cccggaatcc
     2641 accgagccat acgaaacgcg ccttcgacgc gatttcaggg gcgcgaagaa gcgcggaggc
     2701 aagatcagtt gccggaccgg ttccgatcac gaacaacggc tccggatctt cggtcagagc
     2761 acggtcaatg atgaaatcca ccccttcagc cgagcccggg ctcaaattgt tgccgagagg
     2821 gcgctccaca cctcgcaggt tcggcacctc aggccgtcca agagccgcta ggatggccgc
     2881 cgtctcttcc tggtagatcg tcaccgattc gggcccgttg gcatgcgtat tctgaacatt
     2941 gacgatcgcc tcaatccgca catctttcgc gaacgcatat gtgatcgcga actgatcgtc
     3001 aatttcgttt ttcgtgtcgc aatccaggat aacgcgcatc gtcacactgc cttttacaga
     3061 agcacgtcag catcgccgcg tctaaggtgt tgtgccgcaa ggcacacccg cgcgaaatag
     3121 ccgtccacag acggcaggaa atctgcattt gtcagaaccc gagcaccggt tgtcgtcgca
     3181 atgcccggat tcgttgagta gaaaactgga gcgactaacc ggttcacg
//
LOCUS       DAOTNG010000340         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23555_length_3220_cov_4.267299, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000340 DAOTNG010000000
VERSION     DAOTNG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23555_length_3220_cov_4.267299"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="P5179_12840"
     CDS             <1..842
                     /locus_tag="P5179_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRS96147.1"
                     /translation="IKGREEILRVHARKVTLAPDVDLHQIAARTPGMVGADLANMINE
                     AALLAARKNHPAVTMEDLNEAIDRGIAGLERKSRVLNETERRITAFHEAGHALVAEAL
                     PHTDPVHRVSIIPRGVTSLGQTTYQPTEDRYLMSKEELEDRLAVALAGRVAEEIVFGD
                     VSTSAGTDFRRATRIAQHMVKDYGMSRLGLVSYGEAPGFLAQPVGGGAVHYSEETAAE
                     IDDEVRRIVSEGYQRVRELLENNTDALHRLANALIERENLQGDEVREILRGSLKETET
                     SAA"
     gene            903..1691
                     /locus_tag="P5179_12845"
     CDS             903..1691
                     /locus_tag="P5179_12845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS96148.1"
                     /translation="MSGFAFRRRFGRLAHLLRAHDPAKLPPEHLFAPLEEERGPEVGR
                     IEALRKELLSSTATISDFHFGSGEAVASRVTRVAHECRYSSVPARWCRVLFRLVRKTK
                     PANCIELGTNLGISSAYLAAALKLNGSGRLFTIEGSPSLTECAKRNMTLLGYDNVQIV
                     NSSFETGLPDTLKEAGSVDFAFVDGHHDERATLDYAGMIIEASSEGAILVFDDIRWSS
                     GMARAWERIAADKSVTAVYDCYDIGAVVVREPGATRKEFTRIRA"
     gene            complement(1694..2353)
                     /locus_tag="P5179_12850"
     CDS             complement(1694..2353)
                     /locus_tag="P5179_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRS96149.1"
                     /translation="MRARDNDKLTIVWYGLVRNASTVNPVDRMLRDGATVSPGEGIQL
                     FVSVKPAAFLYVIHQSPTGLLTVWWPSQSSGFNAYLEKERVLTLPARGKIHVLGAAQG
                     TEAVYLIAAFKPVTRVDKLTKEIQWLFASAQAFGSGVPARQVKQMLPRGLVWRLDANT
                     PVGLTDQRVPVATTVRCIGRIAGGGTATSTSDSQNTVIPEQVRGGDVVARVIRFQRQL
                     R"
     gene            complement(2495..>3220)
                     /locus_tag="P5179_12855"
     CDS             complement(2495..>3220)
                     /locus_tag="P5179_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS96150.1"
                     /translation="LNHVRDDGNETNHVLKDVTFQAPRGSVLAIVGKSGSGKTTLAYH
                     FNRLLEPTSGEVRIGERPARSIALHELRRIVGLTFQRVDLQLFEASVLDDVAFGLVQR
                     GMERAEAYRRARLFLHRMGLDPDSFGNRAPGTLSTGEKRRVALAGVLVSEPEVIVLDE
                     PTSGLDAASVEQLSEIVSELANQGKTLLIITHDLNFAARTADGVILVDEGRAEVLEGT
                     DRLGELAATWLAETLSRFRSSAA"
BASE COUNT          683 a          937 c          959 g          641 t
ORIGIN      
        1 atatcaaagg ccgtgaagag atactgcgcg tgcacgcccg taaggtaaca ctggctccgg
       61 atgttgatct gcatcagatc gccgcgcgga ccccgggtat ggtgggcgcc gacctcgcca
      121 atatgatcaa cgaggccgcg cttctcgctg cccggaagaa ccaccctgcg gtaaccatgg
      181 aagacttgaa cgaggccatt gaccgtggca tcgcggggtt ggagcggaag agccgcgtgc
      241 tgaatgagac ggagcgacgc atcactgcgt tccacgaagc gggacacgcg ctggtcgcgg
      301 aagccctgcc tcacactgac ccggtgcatc gtgtttcgat cataccgcga ggtgtaacct
      361 ctctcggcca gactacgtac caaccgacgg aagacaggta tctcatgtcc aaggaggagc
      421 tggaggaccg ccttgcggtt gcgcttgccg gccgcgtagc cgaagagatt gtgtttggtg
      481 acgtatcaac aagtgcggga acggatttcc gtcgtgccac ccgcatcgcg cagcacatgg
      541 tgaaggatta cggtatgagc cgtctcgggc tggtgtcata cggggaagct cccggattcc
      601 ttgcgcaacc ggtcggcggg ggcgcggtgc actacagcga agaaaccgcg gcggagatag
      661 atgacgaagt gcgaagaatt gtgtctgaag gatatcaacg agttcgagaa ctccttgaaa
      721 acaacacgga tgcgttacac cggcttgcaa atgcgctgat cgaacgagaa aacctgcagg
      781 gggacgaggt ccgcgagatt cttcgaggca gcctgaagga aactgagacg tcggccgcat
      841 gagcgatggt tctgcaccgc cacgcacgcg caccgcaaga cccagggaaa ggtaacggag
      901 ggatgtccgg cttcgcattc cgtcggcgct ttggcagact ggctcattta ctacgcgcgc
      961 acgaccccgc aaaactacct ccagaacacc ttttcgcgcc gcttgaggaa gagcggggac
     1021 ctgaagttgg ccggatcgaa gccctgcgca aggaacttct cagctccacg gctaccattt
     1081 cggatttcca cttcgggtct ggcgaagccg tggcttcacg ggttacccga gtggcgcatg
     1141 agtgtcggta ctccagtgtg cccgcgcggt ggtgccgggt tcttttccgc cttgttcgaa
     1201 aaacgaagcc tgccaactgc atcgaacttg gcacgaatct ggggatctct tccgcgtacc
     1261 ttgctgctgc actcaaactg aacggatcgg ggcgcctgtt taccatcgag ggatctccct
     1321 cgctcacgga atgcgcgaag agaaacatga cactgctggg atatgacaat gttcagatag
     1381 taaactcgag tttcgaaaca ggcttgccgg acacgttgaa agaggcgggc agtgtagatt
     1441 tcgcgtttgt cgacggccac cacgacgaaa gggcgacgct ggattacgct ggaatgatca
     1501 tcgaagcctc gtcggaaggg gccattctgg tgtttgacga catccggtgg tcttcgggaa
     1561 tggccagggc gtgggagcgg atcgcggctg acaagtccgt cactgccgtg tacgattgct
     1621 acgatatcgg cgcggtggtc gtccgggaac ccggtgccac gagaaaggaa ttcacgcgca
     1681 tccgggcatg aattcagcgc aactgacgct ggaatcggat tactcgtgcg acgacgtcgc
     1741 cacctctgac ctgttccgga atcacggtat tttgcgagtc ggaagtgctc gtggcagtgc
     1801 cgcccccggc gattctcccg atacaccgaa ctgtggtagc gacaggaact cgctggtcgg
     1861 taaggcctac cggagtattt gcatcaagtc gccagacaag accccttgga agcatctgtt
     1921 ttacctgtcg cgccggcacc cctgaaccga aggcctgggc ggaagcgaac agccattgga
     1981 tttccttcgt cagcttgtcc acgcgggtga caggtttgaa ggccgcaatc aaatacaccg
     2041 cctccgttcc ctgggcggct cccagcacgt gaattttccc gcgcgccgga agtgtcagta
     2101 cacgttcttt ttccaggtac gcattgaatc ccgaactctg tgagggccac cagacagtca
     2161 gaagtcctgt cggggactgg tggatgacat agaggaacgc cgccggtttg accgaaacga
     2221 aaagttggat tccctccccg ggcgaaaccg tggcgccatc ccgcagcatt cggtccacag
     2281 ggttcacggt ggaggcattt cttacaagcc cgtaccacac gatggtgagc ttgtcattgt
     2341 ctctcgcgcg cattgctcca cacgagaacg ccatcaacgc ggaggcaaat gaaagaagga
     2401 gggagacaat tcgcagcaag accgtacccg cgttcgatcg ttggtgggac tgcgttcccg
     2461 gacaacccga atgaatgtga tgacgcggtc agggtcaagc cgctgaagac cggaaacggc
     2521 tcaacgtttc tgcgagccat gtggcagcca gttcgccaag acgatctgtg ccttccagca
     2581 cctcggcgcg gccttcatcc acgagaatga caccgtcggc tgtgcgcgcc gcgaaattga
     2641 gatcgtgcgt gattatcagc agcgtcttgc cctgatttgc caactcgctc acgatttcgg
     2701 acaactgttc cacacttgcg gcatcaagtc cggacgttgg ttcatcaagg actattacct
     2761 ccggttctga gacgaggact cccgccagtg ccacccggcg cttctctcct gtactcagcg
     2821 ttcccggcgc gcgattcccg aaactgtccg ggtccaggcc catccggtgc aggaaaaggc
     2881 gagcccgtcg gtacgcctcc gccctctcca tcccacgttg taccaatccg aaggccacgt
     2941 cgtccagcac agaggcttcg aacaactgca ggtctaccct ctggaacgtc aatcctacga
     3001 tccgtcgtag ctcatgcaga gcaatgctgc gggcgggtcg ttctcctata cggacttctc
     3061 cgctggtcgg ttcgagcaac cggttgaaat gatatgccag ggtcgtcttt ccggaacccg
     3121 atttgcccac tatcgcgagc acgctcccgc ggggggcctg gaaggtcacg tctttcaaga
     3181 cgtggttggt ttcattccca tcatcgcgca cgtgattgag
//
LOCUS       DAOTNG010000341         3204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23711_length_3204_cov_3.446491, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000341 DAOTNG010000000
VERSION     DAOTNG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23711_length_3204_cov_3.446491"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5179_12860"
     CDS             <1..248
                     /locus_tag="P5179_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96151.1"
                     /translation="FEIEHSTDMHRSLLKFVELQDFFTNFRIVADEVRRREFQGKVSL
                     RAFSAINRRVEFWCYEDVAKLHSKTSEYASIEARMNL"
     gene            414..956
                     /locus_tag="P5179_12865"
     CDS             414..956
                     /locus_tag="P5179_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96152.1"
                     /translation="MGAGALEVASARRGLNFEKTTDLATVLCELPGYDVERVAGKGYQ
                     NVFFEGKLVARLLRRHDFYRFLDEGGVDWRNKVNGKLLPDQALLVIARETLFVVEIRC
                     QQRPGWVFRKLQTCDFKRKQYVKLVAPLELRVEYVYVLNDWFKKPEYKDVLDYIQSVN
                     CHYKFNELPLAWLGLPVKDA"
     gene            1109..2062
                     /locus_tag="P5179_12870"
     CDS             1109..2062
                     /locus_tag="P5179_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS96153.1"
                     /translation="MSRYTRANTDWLAGCTVGIGIHWTAQTAPRRGNALPFAEAVDRF
                     RCDEFLAAVETAGADYVLFTATHALQMLPCPHPVLDSILPGRTAERDLIGELAAKLRS
                     RGKRLIVYYNHSCNGGDDPAWEQAVGYHDPDKHRLAKNLLAIVRWMGERYGSLIAGWW
                     FDSPYSLDPRGPHNSITTDLRGFQFPWEQMTAAAKAGHPGRLVTYNAGIGETFLYTTH
                     QDYWAGELTDLSTPPVGRYLDNGLQWHGWTALDDRRWVYTDNTTGPHPPLYSDGRLVA
                     FLSACRQAQAPMCFNVVSFQDGALAESSVRQLERITAVLKG"
     gene            2194..2544
                     /locus_tag="P5179_12875"
     CDS             2194..2544
                     /locus_tag="P5179_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HRS96154.1"
                     /translation="MESPASESPKISVRPLVSVALFCAGVWLVPSGIALHFASHDGAT
                     RWSHLFMTIHNTASFLFLIAAVVHVVLNRKALAHYVNAKVGEYSRFKTELLVAIGGVS
                     AFVVLIALHALHVP"
     gene            2632..>3204
                     /locus_tag="P5179_12880"
     CDS             2632..>3204
                     /locus_tag="P5179_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endopolygalacturonase"
                     /protein_id="HRS96155.1"
                     /translation="MPARPYSVPGEVPIADDIICLVDGQRAPVQLCRVSAVPFNRRWP
                     GHQRQIEQSEIAYFASFEMTAPVTVQVRPARAFKDVVLRPLSKHVHPQIDGDTISFSL
                     PSAGGYSLEMDGYHHNLHLFADPPAKYAVDLYDPATIYFPPGCHDVGILQLKDGQTVY
                     LDEGAVVYGCIHATDCRGIRILGRGVLDNSK"
BASE COUNT          665 a          946 c          930 g          663 t
ORIGIN      
        1 tatttgaaat agaacattcc acggacatgc acaggtctct tctgaagttt gtggaacttc
       61 aggatttctt caccaatttt cgcatagttg ctgatgaagt caggaggaga gaatttcaag
      121 gcaaagtatc attacgggcc ttcagtgcca taaacaggcg tgtcgaattc tggtgctacg
      181 aagatgtcgc caaactccat tcaaagacaa gcgagtacgc aagtatcgaa gcaaggatga
      241 atctgtaacg gaacatgcac aaagtccgaa acggctcgtg ttgcgtgctg tgttcccaag
      301 cgcgtccgcg ttgcgtgtgc cctcgttaat gagtttgtca cgcggtcggc aaagcggcag
      361 cagaacgctg tcgacgagaa gttgttgtaa ttggattgtg cggaggcatc cgagtgggag
      421 caggtgcatt ggaagttgcg agcgcacgta ggggattgaa tttcgaaaaa acaacggacc
      481 tagcaacggt cctgtgcgaa ttgcctggtt acgatgttga aagggtcgcc ggcaaggggt
      541 accaaaacgt gtttttcgaa ggcaagctgg ttgcgaggct tctcagaaga cacgattttt
      601 acaggtttct ggacgaaggt ggagtcgatt ggcggaacaa agtcaacggg aaattgctcc
      661 cagaccaagc gctcctggtg atcgcacggg aaaccttgtt cgtcgtcgag atccggtgcc
      721 aacagcgacc agggtgggta ttcagaaaac tgcagacctg tgatttcaag cgaaaacagt
      781 atgtcaagct cgttgctcct ttggaattgc gcgtcgaata tgtctacgtg ctgaacgatt
      841 ggttcaaaaa accagaatac aaggatgtgc tagattatat ccaaagcgtc aactgccatt
      901 acaagttcaa tgagctgccc cttgcctggc tcggtttgcc cgtgaaggac gcttgaccgg
      961 gcaccgaaat ggatgcgttt tgaacgacct ctcacgcacc ctttccactc cgtccaccat
     1021 tcccttcgcg tcttcgtgtc ttcgtgtgag atgtccaccg ttgctcactg agaactcaga
     1081 atcaaccaac gcaatgaatt gagaggcgat gagcaggtac accagggcca acaccgactg
     1141 gctcgccggc tgcacagtcg gaatcggcat ccactggacg gcgcaaaccg cgccacgccg
     1201 cggaaacgcc ctgccattcg cggaggccgt ggaccggttt cggtgcgacg agttcctcgc
     1261 cgccgtcgag acagcgggtg cggactacgt tctgttcact gcaacgcacg ccctgcagat
     1321 gctcccctgc ccgcacccgg tgctggattc catcctgccc ggcagaacgg ctgagcgcga
     1381 tctgatcggt gaactcgcag cgaagcttcg gagccgcggc aagcgtctga tcgtctacta
     1441 caatcactcg tgcaacggcg gcgacgaccc ggcgtgggag caggccgtcg gctaccacga
     1501 ccccgacaag caccggttgg cgaagaacct cctcgccatc gtgcggtgga tgggcgaacg
     1561 atacggctcc ctgatcgccg gttggtggtt cgacagcccc tactccctcg atccgcgcgg
     1621 cccgcacaac tccatcacca ccgacctgcg cggcttccag ttcccgtggg aacaaatgac
     1681 ggctgcagcg aaagccggcc atccgggccg gctcgtgacg tacaacgcgg gtatcggcga
     1741 gacatttctc tacaccacgc atcaggatta ctgggcgggg gaactgaccg acctcagcac
     1801 ccctcccgtc gggcgctatc tcgacaatgg cctgcagtgg cacggctgga cggctctcga
     1861 cgaccggcgc tgggtgtaca ccgacaacac gacgggaccc catccgccgc tctactccga
     1921 cgggagactg gtggcatttc tgtcggcatg ccgccaggcg caggcaccga tgtgcttcaa
     1981 cgtcgtctcg tttcaggatg gggcgctcgc agagtcgtcc gtgcggcagt tggaacgaat
     2041 cactgcggtg ctgaaaggat gaagggaagg ccttcaagcc cccttcgcgt cttcgcgtct
     2101 tcgtgtcttc gcgtgagata ttatacccgt tggattcgcc attttgcccg accacatgcg
     2161 ccaaggaaat tgccaccgga agagggtgcg acaatggaaa gcccggcatc ggaatctccg
     2221 aagatcagtg ttcggcccct cgtctctgtg gccctgttct gcgccggtgt gtggctggtc
     2281 ccttccggca tcgcgctcca cttcgcctcg cacgatggcg cgacccggtg gagccacctg
     2341 ttcatgacga ttcacaatac tgcctcgttt cttttcctga ttgcggccgt tgtgcacgtg
     2401 gtcctgaacc gcaaagccct cgcgcactac gtgaacgcga aggtcggaga gtattcgagg
     2461 ttcaaaacgg agttgcttgt cgccattggg ggcgtgtcag cgttcgtggt cctgattgcc
     2521 ctccacgcgc tgcacgtccc atagcggcaa tcgatccact tggaggggcg tcgtccgcat
     2581 aacggacgca gcgctttgtc gggcccatga ggttacacga aggagtctta aatgcccgcc
     2641 cgcccatact cggtacccgg ggaagttcca atcgccgacg acatcatctg cctggttgac
     2701 ggccagagag cgccggtgca gctctgccgc gtatctgccg tgccgttcaa tcgccgatgg
     2761 ccgggacacc agcggcagat cgagcagtcc gaaatcgcgt atttcgcctc attcgaaatg
     2821 accgcgccgg tcacggttca ggtgaggccc gcgcgcgcct tcaaggacgt cgtcctgcga
     2881 cctctgtcga agcacgtaca tccgcagatc gatggcgata ccatcagctt ctcactaccc
     2941 tctgcaggcg gctactcgct ggagatggac ggctatcacc acaatctgca tctgttcgcc
     3001 gatcctcccg cgaagtacgc ggtggacctt tacgaccccg cgaccatcta cttcccgccg
     3061 ggctgccacg atgtcggcat tctgcagctc aaggatgggc agacggtgta tctggacgag
     3121 ggcgctgtcg tctacggctg catccacgcc accgattgca ggggcatccg catccttggc
     3181 cggggtgtgc tggataacag caag
//
LOCUS       DAOTNG010000342         3178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23937_length_3178_cov_4.892411, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000342 DAOTNG010000000
VERSION     DAOTNG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23937_length_3178_cov_4.892411"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..225
                     /locus_tag="P5179_12885"
     tRNA            150..225
                     /locus_tag="P5179_12885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Glu,seq:ctc)
     gene            complement(277..720)
                     /locus_tag="P5179_12890"
     CDS             complement(277..720)
                     /locus_tag="P5179_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRS96156.1"
                     /translation="MLAETARQNPYAQSADVIAAVPLHRVRERERAFNQSDLLATAVA
                     AALGKPLASTALVRTRATPRQATIQSRAERMANVRGAFHVKDPRAICGKTVLLVDDVI
                     TTGATVCALAETVLEAGALAVSAAAVAHPFASGPMETYDPQIWTV"
     gene            complement(1020..3032)
                     /locus_tag="P5179_12895"
     CDS             complement(1020..3032)
                     /locus_tag="P5179_12895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96157.1"
                     /translation="MVSPLPVLEDKTLFARGDTSITPAPEEISRLVIYLVKPSRYDDD
                     GYLVRHWRGVLPSNTLSCLAGLTDDVVQRKVLGEHLKISTVLVDESVERMPRRKILAD
                     AGRKGTRAVVCLAGVQTNQFARATDIALEYASLGVRVMIGGFHVSGMTAMFPEGTPDI
                     ARLTDAGVTVVAGEVEDRWAALLSDVVNDRLQPLYNFLATPPELKRQPVPRVKRSYMK
                     RFAVSNLGTIDTSRGCPFACTFCTIINVQGRKMRCREASDLRNTIIENYNQGINFYFF
                     TDDNFARNKNWESILDLIIDLNNEGRHITFMMQIDVRAYKIPGFVEKSKRAGCSQVFV
                     GMESINPDNLKAAGKTQNDVGDYAHMVEVWHDAGVAVHVGYIIGFPFDTPESVADDVR
                     RLADEMKVDQASFFMLTPLPGSVDHKRMVENGEEMDADFNRFDSFHAATNHPRMTKAQ
                     WFETYQRAWRDFYRFDRMRAIMRRTPRRHYWNVFTNFMWYKNSFLSEDAHPMVTGFIR
                     RKKRCERRPGMPREERWSYFLNRLREIKKELVTRWSLSRELQELWLQTRRRTEVENRV
                     ADALHELALQRKRIRFRIEDWKAAYSAAQAQVPSAWKIVVAKVNVLSLRWTYTRQDLD
                     NFWRQTRRQWECRAFHRINWPLATWYAFRDAVLSLRFALHMALRLQ"
BASE COUNT          644 a          933 c          902 g          699 t
ORIGIN      
        1 gacgcaggct cagccgcctg tcggtaccga tcggtacgcc tgaaaaacgg agacgaccag
       61 ttctggttca atcgcaattc gcgtgggccg gggcccgcgg cttttggcaa acgccgggtt
      121 tggcgttaac tttaatattc ttccgctttg gcgcgttcgt ctagtggccc aggacgctgg
      181 cctctcacgc cggaaacacg ggttcgactc ccgtacgcgc taccattgct cgaatcccct
      241 tcgcttccga attcgtcacc tacaagacag tgattttcac accgtccaga tttgcggatc
      301 gtacgtctcc atcggcccgg atgcgaaggg atgtgcgacc gccgccgcac tgactgccag
      361 agcaccggcc tccaacacag tttctgccag tgcgcagacc gtcgcaccgg tggtgatgac
      421 atcgtccacg agaagaaccg tttttccgca aatagctcgt ggatccttca cgtgaaatgc
      481 accccggaca ttagccatgc gttcagcgcg ggattgtatg gttgcctggc ggggagtagc
      541 tctcgtacgc acgagggcgg tgcttgccag gggctttccg agtgcggccg ccactgccgt
      601 cgcgagcaaa tcggattgat tgaacgcgcg ttctcgttca cgcaccctgt gcaacggaac
      661 tgcggcgatc acatcggcgc tctgcgcgta ggggttctgt cgggccgttt ctgccagcat
      721 gtctgcgaga aaacgcccga cagatcgatt acgcccatac ttacaggcaa agacgagttc
      781 ccttgcccgc acgtattccg tggctgcgat caacgctgtc agaggtgtgg gaagatttgc
      841 gcacgagtgg cacgggatcg ggtaggaaca acgctcgcag cgggcctgca aaggtgccag
      901 ttgggtcgtg caggatgcgc acaatcgctg agcggtttcc agaggtcttt ggcacaccgg
      961 acacgcgggc ggccagatgg cgtcaaggaa cgcttctgac acgcgccgcg cgagcattct
     1021 cattgcagcc ggagtgccat atggagggcg aagcgcagac tgagcacggc gtcccggaac
     1081 gcgtaccacg ttgcaagcgg ccaattaatc cgatggaatg cccggcattc ccattgtctt
     1141 cgggtctgac gccagaagtt gtccagatct tgcctcgtgt aggtccatcg gagcgagaga
     1201 acgttcacct tcgccacaac gattttccat gccgaaggaa cctgcgcctg cgccgcgctg
     1261 tatgccgctt tccagtcttc gattcggaac cggattcgct ttctctgcaa agcgagttcg
     1321 tgcagtgcat cagccacgcg attttccact tccgtgcgcc ggcgcgtctg aagccagagc
     1381 tcttggagct cgcgcgaaag gctccaccgg gtcacaagct ctttcttgat ttcacggaga
     1441 cggttcagga agtaactcca gcgttcttcg cgcggcatcc ccggtctccg ttcgcaacgc
     1501 ttcttcctgc ggatgaatcc ggttaccatg ggatgcgcat cttcggaaag gaaagagttt
     1561 ttgtaccaca tgaaattggt gaacacgttc cagtaatgcc gccgtggcgt ccttcgcatg
     1621 atggcccgca tacggtcgaa ccggtaaaaa tctcgccagg ctcgctgata tgtttcgaac
     1681 cattgcgctt ttgtcatgcg ggggtggttc gttgcggcat ggaatgagtc gaagcggtta
     1741 aaatccgcgt ccatctcttc cccgttttcc accatacgct tgtggtcaac ggaacccgga
     1801 agaggtgtga gcatgaaaaa ggaagcctgg tccaccttca tttcgtccgc caggcggcgg
     1861 acatcatccg cgaccgactc cggggtatcg aacgggaagc cgataatgta gccgacgtga
     1921 accgccactc ccgcatcgtg ccagacctca accatgtgag cgtaatcacc cacgtcgttt
     1981 tgcgtttttc ccgctgcttt caggttgtcc gggttgatac tttccatacc gacaaacacc
     2041 tgagagcatc ccgccctctt cgatttctcc acaaacccgg gaatcttgta cgcccgcaca
     2101 tcaatctgca tcatgaaggt tatgtgacga ccctcgttgt tcagatcgat gatcaggtcg
     2161 aggatggatt cccagttctt gttccgggca aaattgtcgt cggtgaagaa gtaaaagttt
     2221 atgccctggt tatagttttc aatgatggtg ttgcgaaggt cgctcgcctc tctgcatcgc
     2281 attttccgcc cctggacgtt gataatggtg cagaacgtgc aggcaaacgg gcagccgcgg
     2341 ctggtgtcta tagtaccaag atttgaaacg gcgaagcgtt tcatgtagga ccgtttcact
     2401 cgcggaaccg gttgtcgttt gagctccggc ggcgtagcaa gaaagttgta caatggctgc
     2461 aggcgatcgt tgaccacatc tgacagaagg gccgcccatc gatcttccac ctcgccggca
     2521 acgaccgtca ctcccgcatc cgtcaaccgc gcgatgtccg gcgtgccctc cggaaacatg
     2581 gccgtcatgc cgctgacgtg aaacccaccg atcatcacgc gcacacccag cgatgcatat
     2641 tccagcgcga tgtcggtcgc gcgtgcgaac tggttcgtct gaacgccggc gaggcagaca
     2701 accgcacgcg ttccctttcg acccgcgtcc gccaggattt tccgccgcgg catccgttcc
     2761 actgactcat cgaccagaac cgtgctgatc ttaagatgtt cacccagcac ttttcgttgg
     2821 accacatcgt cagtcaaccc cgcaaggcag gaaagcgtat tgctgggaag aaccccgcgc
     2881 caatggcgaa caagatatcc gtcgtcgtcg tagcgcgatg gtttgacgag gtaaatgacg
     2941 aggcgggaga tctcttcagg tgccggggtg atcgatgtat cgccgcgcgc gaaaagcgtt
     3001 ttgtcttcga gaaccgggag tgggctgacc atgaatgtgt gcgtcctgtt ctgattgatt
     3061 gtggtgccga atactcaaac gtgaggttgc agcttcgtac tacaacccaa gcctgaatgt
     3121 atggcaatcg cgggccgaac gccaagttcc cctcgcgccg gtcaggcagg gagatcag
//
LOCUS       DAOTNG010000343         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_23975_length_3173_cov_3.418858, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000343 DAOTNG010000000
VERSION     DAOTNG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23975_length_3173_cov_3.418858"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5179_12900"
     CDS             complement(<1..207)
                     /locus_tag="P5179_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96158.1"
                     /translation="MTDLERFVAICRGETPDYIPIFGFPGAPGMSDGAMRKTHERLVA
                     TGMPSSVGGASSLIYGNPERESWRR"
     gene            complement(227..1609)
                     /locus_tag="P5179_12905"
     CDS             complement(227..1609)
                     /locus_tag="P5179_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96159.1"
                     /translation="MPHNVGTRRELFVDGTLVQSFQGTRLVLQKPERRETVLELDAPW
                     ENNVAFPYRVLPFKGGWRMYYRASILDLSREEDTCVTAVAESDDGVSFRRPSLGVCEF
                     AGSRQNNILQIGGFPNTPPPFFDTRPGCPPEERFKGIAARQSQAFAMVSGDGVRWRLL
                     HNTPMHLRGQFDTVNTAFWDELAGCYRSYTRSWHDPETGAAFPTWDFGGVAAVRAIQH
                     ARSEDFLNWTEPEQLQYADGDRTVHLYTNAIVPCPEAEHILLGFPSRFVPDRTPDESH
                     GLPGVNDALFMTSRDGVHWNRWLDAWVSPGLDPLNWTERNNYPVWGIAKTSPTEWSMY
                     ISEHYRHEGVPTRVRRLSIRPWGFVGVHADYSGGELVTKPFVFEGTSLSLNARTSAAG
                     AIRVEIQDETGHAIPAFSLAEMRPWYGDSLDARMAWGENTSLGSLRGRPIRLRFWMKD
                     ADVFSFRFPS"
     gene            complement(1629..>3173)
                     /locus_tag="P5179_12910"
     CDS             complement(1629..>3173)
                     /locus_tag="P5179_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96160.1"
                     /translation="TDGFWMDGFDYTGYPSRACFCHFCRERFAKDNAGMSLEAAYLSD
                     KLRLWQRDVFLELMKDIVTIANRGRADRVVVYNNAGTNLELGYERIDELCSLNSMEAH
                     SALGKSLTARLLAGQRKAFEIYTPVADKVFSWTPRTTPLLKLETAIVAAHGGSTLAGL
                     DVTASGRIPGYQTRQMAEVGAFVRRVAGVCHEAEPVYDVGVLIPKDQWKDELGTWAVC
                     LLKHHHAFCQVPLHVRDFSIFRVVVVADGYEMTPDLAAALEEYASQGGNVVVECNGAG
                     VCTEYGDMFLLSKLLGVSANGRHGFDVSYIGGIAGELADGLWDGPIRAGADGFRVVPV
                     TARVLARYVYPVARYSSQRWIWREPNPPRAEISDESAITVNHFGKGKAVFVACPAGLD
                     EAPQRRGVMKLCSNIVSYLCPEELWRTNAPSGVEIVLSKKEGKYLLHVLNHYVEQSSR
                     FDTGDDSIPSLAGFDILVNTKRVGSLGKVTRASTGASVPATRINDYMRIHVDTLAIQE
                     MYLLEP"
BASE COUNT          684 a          993 c          833 g          663 t
ORIGIN      
        1 tctcctccag ctttcccgtt cagggttacc gtaaatgagg ctcgacgccc ctccgacgga
       61 cgaaggcatt cctgtggcga ccagtcgttc gtgcgttttg cgcatggcgc cgtcgctcat
      121 tcccggtgca cccggaaaac cgaaaatggg tatgtaatcg ggtgtttcac cccggcagat
      181 cgcgacaaat cgttccaggt cagtcattta ctttttcgag ttcctttcag gaaggaaaac
      241 gaaacgaaaa cacgtcagca tccttcatcc agaagcgaag ccgtattggc cgcccgcgaa
      301 gggacccgag gctggtgttt tcgccccagg ccatccgcgc atcgagcgaa tcgccgtacc
      361 acggccgcat ctcggcaagg gagaacgccg gtattgcatg gcccgtttca tcctgaatct
      421 ccacgcggat ggcgcctgct gcggaggtgc gtgcgttcag actcagcgaa gttccctcaa
      481 agacgaacgg cttcgtgacc aactcgccgc ccgaatagtc tgcgtgtacg ccaacaaatc
      541 cccatggccg gatgctcaac ctgcgaacgc gcgtcggcac gccctcgtgg cgataatgct
      601 cggagatata catggaccat tcggtgggcg aggtcttcgc aattccccag acggggtagt
      661 tgtttcgttc cgtccagttc aacgggtcca gccctggcga cacccacgca tccagccaac
      721 gattccagtg caccccgtcc ctgctcgtca tgaataatgc atcattgacc ccgggcaacc
      781 cgtgggattc gtccggtgtt cgatctggaa cgaaccgact cggaaaacca aggaggatat
      841 gttccgcttc cgggcatgga acgatcgcgt tcgtgtacag atggactgtc cggtcgccgt
      901 ccgcgtactg cagttgttcc ggctccgtcc agttcaagaa gtcttcggaa cgcgcgtgct
      961 ggatcgcccg gaccgcggcg acgccgccaa aatcccacgt cgggaaggct gcgccggttt
     1021 cagggtcgtg ccagctgcgc gtatatgatc ggtagcaccc ggcgagttcg tcccagaagg
     1081 ccgtatttac ggtgtcgaat tgcccacgga ggtgcatcgg tgtgttgtga agcaggcgcc
     1141 accgcacgcc gtcgccggag accatcgcaa aggcctggga ttgccgtgca gcaattcctt
     1201 tgaaacgctc ctccggagga cagcccggtc tcgtatcgaa aaacggcggc ggcgtgtttg
     1261 gaaaacctcc gatctgcaga atattgttct gcctggaacc cgcaaactcg cacaccccca
     1321 gagaaggtcg gcggaaactc acgccgtcat cgctttcggc aacggcagta acacaggtgt
     1381 cttcctcccg ggaaagatcc agaatggacg ccctgtagta catgcgccac ccgcctttga
     1441 agggaagaac cctgtaaggg aaggcaacat tgttttccca cggtgcgtcc aactccagta
     1501 ccgtctcgcg acgttccggc ttctgaagaa caagtcgggt gccctggaac gactgcacga
     1561 gagttccgtc aacgaagagc tcccgccttg ttccgacgtt gtgtggcatg gtgctgtctc
     1621 ctctggaatc acggttccaa gagatacatt tcctgaattg ccagtgtatc tacgtgtatg
     1681 cgcatataat cattaatgcg tgttgctgga actgatgcgc cagttgaagc acgtgtcact
     1741 ttccccaaac tcccgacccg tttcgtattc acgagaatat cgaaacccgc gagtgaggga
     1801 atcgaatcat cgcccgtgtc gaaccgactg ctttgctcaa catagtggtt cagtacgtgg
     1861 aggaggtatt tcccttcctt cttgctcagc actatttcca cacctgaagg agcgttcgtg
     1921 cgccacaatt cctccgggca gaggtacgag acaatgttcg aacaaagctt catcacacca
     1981 cggcgttgcg gcgcttcgtc aagacctgct gggcacgcca caaacacggc tttccccttg
     2041 ccaaaatgat tgacggtgat ggcggattcg tctgaaatct ccgcgcgcgg tgggttcggc
     2101 tctctccata tccaccgttg tgacgagtaa cgggccaccg gataaacata acgggcaaga
     2161 actcgagccg ttacgggaac cactcgaaag ccgtctgcgc cggcgcgaat ggggccatcc
     2221 cacagcccat ccgcgagttc tccggcgatt cctccgatgt acgaaacgtc gaaaccatgg
     2281 cggccattgg ccgaaacacc cagcagcttt gagagaagaa acatgtcccc gtactcagta
     2341 cacacccccg cgccgttgca ctccaccacc acgttcccac cttgcgacgc atactcttca
     2401 agagccgccg ccagatccgg agtcatttcg taaccatccg ccaccacaac aacacggaat
     2461 atggaaaaat cccgcacatg aagtggaacc tggcagaacg catggtggtg tttcaacaga
     2521 cacactgccc acgtacccag ttcgtccttc cactggtcct tcggaataag caccccgacg
     2581 tcgtacaccg gttctgcctc gtgacatacg ccagccactc ggcggacgaa tgctcccact
     2641 tcggccattt gcctcgtctg gtacccaggg attcgcccgg acgcggtaac atccagcccc
     2701 gcaagcgtcg aacccccgtg agcagccaca atcgccgttt cgagtttcag taacggggtg
     2761 gttcggggcg tccatgagaa caccttgtct gcgaccggtg tgtatatttc gaacgccttt
     2821 cgctgccccg ccagcaaccg cgctgtaagg cttttcccaa gagcagaatg cgcttccatg
     2881 gaattcagcg aacaaagctc gtcgatccgt tcgtacccga gttcaaggtt tgttccggcg
     2941 ttattgtaca cgactacacg atccgcccgt ccccgattcg cgatcgtcac tatgtccttc
     3001 attaattcca gaaacacgtc cctttgccac agtcgcagct tatctgacag gtacgcggct
     3061 tccagtgaca tccctgcatt gtctttcgcg aaccgctcgc gacagaaatg gcagaaacac
     3121 gcccgcgacg ggtatccggt atagtcgaat ccatccatcc agaagccgtc cgt
//
LOCUS       DAOTNG010000344         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24053_length_3164_cov_6.610164, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000344 DAOTNG010000000
VERSION     DAOTNG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24053_length_3164_cov_6.610164"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..2160
                     /locus_tag="P5179_12915"
     CDS             154..2160
                     /locus_tag="P5179_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96161.1"
                     /translation="MVEDLSGTLTLETSDSRRPITVRSVVGGTALIALLAFITPYSEY
                     RVHSMEFFQGQIPVGALASLVVVLLPVNWFLGRFKPAWRISLPELVFMFIMAFAGIMV
                     YHIGMMGLFLSMVSSPDYFAAPENRYAEFILPYLPPAAIPSNAHNEMAYFYTGLPPGQ
                     KIPWNVWLVPLFWWWSFFAAFLLVCCALTAILRKQWFDHEKVTFPHAEVTLALVKGSG
                     EKGGLPEIMRSRLFWYGALIPFAFIVWNIISYFTPLWPRIRFSQVETGIAIQYFDSFH
                     VKPDFFTIGFAYFVDTNILFSLWFFRYLVMVQDAAYRRVGVVAATRNDPWTTANALIG
                     WECFGGLVLWTLWGLWMGRHHFRAVWQRVWDSGAGADDSNELLSYRTAVFSLAGGLLF
                     MLVWLMYLGMAWYVATIFLAILMVLIIGITRVVAESGMPIVGAPITAQAFTMRTIGDA
                     NLSPQSFVGLSLSLAAFRMIEGYPMPMVMHSARLGDIVRGRRRALFVAIFAGSIIAMV
                     LMSTTTIRLAYDGGAFNFGAHHAFHQMWEAYDHMVARIKDPWPREPALLLHFAGGASF
                     VASLLFARYRFNWSGLHPVGFFTAMTFYHSVTTLSFLITWLIKLIINKIGGMALYNRW
                     KPFFVGLICGQVAGAVLSFVVDAIFFMGEGHDIYTMAAFGGQGW"
     gene            2194..>3164
                     /locus_tag="P5179_12920"
     CDS             2194..>3164
                     /locus_tag="P5179_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96162.1"
                     /translation="MKVFANTAKRFTDEHSGIEFFLLTPDAVESIPLSYDWPAFSPDN
                     EWVIIRCQFPRESGKPSGFYRIRTDGTEIAHLSEGGIHPRLTPDGRTLFVLHPDSPVL
                     HALDLASGRDDEVCSLEKLLPDGWVFVQMRLSPASGHLFVMLRQPDIMPLRVDLRTGD
                     AVRLDDFDGMVWACAAEKPRVIVVRQRRAERGRRYTYMDYRKLELEPGDRSLWSVDVD
                     GGDEELVCVDYFSHATIHGRTTQVQGCGKWGDRSITICDAGKEPRKVCQGPYFWHSGA
                     SFDAQWIAADTNWPNYGIQLVHVPTGNFGYLCDSGSSLADGLKHPHPG"
BASE COUNT          553 a          923 c          950 g          738 t
ORIGIN      
        1 gagcctttca acgccatttt gtcggggaac gcattgaggc ttgtcccgct ggcggtgtaa
       61 gccaccgcgg agcgtgtccg ttggccatcg tcgcgagccc ttcctcgcga tgatagcctg
      121 ctgaaggtaa cacccttccg gagggagccg ggcatggtgg aggatctcag cggaacgctt
      181 acccttgaaa cgtccgattc cagacgtccg atcacggtgc gttcggtagt gggtggcact
      241 gctctcatcg cgctgctcgc cttcatcact ccgtactctg agtaccgcgt gcactccatg
      301 gagttcttcc agggccagat tcccgtgggg gcgctggcct cactcgtggt ggtgttgtta
      361 ccggtcaact ggtttctcgg gaggttcaaa cccgcctggc ggatatcgct gccagaactc
      421 gtattcatgt tcatcatggc gttcgcagga ataatggtct atcacatcgg tatgatgggc
      481 cttttcctga gcatggtatc gtcgccggat tacttcgccg cacctgaaaa ccgctacgcc
      541 gagtttatcc tgccctacct cccgccggcg gcgatcccca gcaacgcaca caacgagatg
      601 gcctatttct acaccggatt gcctccgggg cagaagatac cctggaatgt gtggctggtt
      661 ccgttgttct ggtggtggtc gtttttcgca gcgttcctgc tcgtctgttg cgcactgacc
      721 gcgatcttga ggaaacagtg gtttgaccac gagaaggtga ccttccccca cgcggaagtg
      781 acgcttgccc tggtaaaagg ttcgggagaa aagggcgggt taccggaaat catgcgcagc
      841 cggcttttct ggtacggcgc gctcatcccc tttgccttta tcgtgtggaa tatcatcagc
      901 tacttcactc cactctggcc gagaatccgg ttttcccaag tcgaaacagg gatcgcgatt
      961 cagtatttcg acagcttcca cgtcaaaccg gactttttca cgatcggttt cgcgtatttc
     1021 gttgatacca acattctctt cagcctctgg tttttccgct atctcgtgat ggtgcaggat
     1081 gccgcctacc ggcgagtcgg cgtagtcgcg gcaacgcgga atgacccgtg gacgaccgcc
     1141 aacgcgttga tcggctggga gtgcttcggg ggccttgtcc tgtggacgtt gtggggcctg
     1201 tggatgggcc gtcaccattt tcgcgcggtg tggcagcgag tctgggattc tggcgcgggc
     1261 gcagacgact caaacgagct gctttcatac cggacggctg tcttttcact ggcgggcggg
     1321 ttgttgttca tgctggtgtg gttgatgtac ctcgggatgg cgtggtacgt cgccacgata
     1381 tttctcgcaa tcctgatggt gcttatcatc ggcatcaccc gtgtagtagc cgaatcaggg
     1441 atgcctatcg tgggagctcc gattaccgcc caggccttca cgatgcgcac aattggcgat
     1501 gccaatctgt ctccccagtc gtttgtcggg ttgtcccttt ccctcgccgc gttccgtatg
     1561 atcgaggggt atccgatgcc catggtgatg cattccgcgc ggctcggcga catcgtgcgt
     1621 ggtcggcggc gcgcgctctt cgtcgccatt tttgcgggct cgatcatcgc aatggtcctc
     1681 atgagcacga ccacgatacg cctcgcctac gacggaggcg cgttcaattt cggggcacac
     1741 cacgcgttcc accagatgtg ggaggcatac gatcacatgg tggcacgcat caaagacccg
     1801 tggccgaggg aacccgcgct tctgctccac tttgccggcg gagcttcctt cgtcgcatcc
     1861 ctcctgttcg cccgctaccg gttcaactgg tccggtttgc atccggtggg gttttttacg
     1921 gcaatgacgt tctaccattc cgtcacgacg ctcagtttcc tgatcacgtg gcttattaag
     1981 ctcatcatca acaagattgg cgggatggcg ctgtacaatc ggtggaagcc cttcttcgtg
     2041 ggattgatct gtggccaggt ggccggtgcc gttctgtcgt tcgtggtgga cgcgatcttc
     2101 ttcatgggcg aaggtcacga catctacacg atggctgcat ttggcggcca gggctggtga
     2161 gacgaaccag tgtggggcga cgcaggggtg cgaatgaagg tatttgccaa caccgcaaaa
     2221 cggttcaccg atgagcactc gggaattgaa ttcttcctcc tgactccgga cgctgtcgaa
     2281 agcattccgc tcagttacga ctggcccgcg ttttcacccg acaacgaatg ggtcatcatc
     2341 cggtgccagt ttcctcggga atcaggcaag ccatcggggt tctaccggat ccgtaccgac
     2401 ggaaccgaaa tcgcccatct ctccgaagga gggattcacc cgcgcctgac tcccgacggt
     2461 cgaactcttt tcgttctcca tccggacagc ccggtgctcc acgcactcga ccttgcttcg
     2521 gggcgggatg acgaggtatg cagcctcgag aagttgctcc ctgacggctg ggtattcgtg
     2581 cagatgcggc tgtctcccgc ctcaggtcac ctgtttgtga tgttgcggca gccggacatc
     2641 atgcctctgc gcgttgacct gagaacgggt gacgcggtcc gacttgacga tttcgacggc
     2701 atggtctggg cgtgcgccgc cgagaagccg cgcgtgatcg tggtacggca gcggcgtgcg
     2761 gagcgcgggc ggcggtacac ctacatggat tacaggaaac tggaattgga accgggcgac
     2821 cgttccctct ggtcggtgga cgtggacggg ggggatgagg aactcgtgtg cgtggactac
     2881 ttctcccacg cgactattca tggccgcacc acgcaggtac aggggtgcgg aaagtggggt
     2941 gaccgaagca tcacgatctg cgacgcaggg aaagagccgc gcaaggtgtg tcagggaccc
     3001 tatttctggc attcaggcgc gtcattcgac gcgcagtgga tcgcagccga caccaactgg
     3061 ccgaactacg gaatccagct tgtccatgtt cccaccggca acttcggata tctttgcgac
     3121 agcggctcgt ccctcgccga tggcctcaaa caccctcatc ccgg
//
LOCUS       DAOTNG010000345         3137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24297_length_3137_cov_4.523037, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000345 DAOTNG010000000
VERSION     DAOTNG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24297_length_3137_cov_4.523037"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5179_12925"
     CDS             <1..270
                     /locus_tag="P5179_12925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS96163.1"
                     /translation="FPASGSPLWRVLKKGWMYVAVPFLVRPFTDAQAYRYLAESIEAH
                     CSAEALVDLLTSCGAESPSIRFLSWCGSSYISVQVNRGRPRPPEL"
     gene            280..1713
                     /locus_tag="P5179_12930"
     CDS             280..1713
                     /locus_tag="P5179_12930"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HRS96164.1"
                     /translation="MIGPGSSRKPCLQDTIVSDEAFSFLREQLSAPIGRNCFRAITVP
                     IERTSLTGALKFDGTETWIYWATPSGEETLAWGAAWKTAPRARDRFAAAERSVSTLWD
                     RIERRSFPGTRKPNLRVFSGFAFDPAGPHDGEWDGFPSNYCVLPGICITCSRGSCRAT
                     VISGRDSYREALHGFSDLLGSLSPTLTGTQEDRAFTLDAAHEATGENQIAQWVRSIAS
                     IKDGITSGRFQKVVAARSVLHSLSRTPDIRKALDLLALRYPDSYRFAAFLGKKLFLAA
                     TPELLVHKRASRFTTHALAGSMPFGANAQAKYAARRALLRSAKDSDEHSLVVEAIRRS
                     VGGFAKTLEVSKSPRIILYGDIAHLETPLEGVLEHDVHVLRLVSAIHPTPAVGGVPSD
                     EALEWIRVNEEVPRGWYAGPIGWFDENGDGCFVTAIRSALVAPGSARLFAGAGIVADS
                     DPVTEYEETEAKLKPMKLALGLSNDVR"
     gene            1700..>3137
                     /gene="menD"
                     /locus_tag="P5179_12935"
     CDS             1700..>3137
                     /gene="menD"
                     /locus_tag="P5179_12935"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRS96165.1"
                     /translation="MTFGDIQFLWARGIVETLLQAGVRCFVVSPGSRSTPLVLALATT
                     RDIVVHKILDERSAGFFAVGYAKATGQTVCLVCTSGSAPAHYLPAIVEAAHSFTPLLV
                     LSADRPPELHGNAAPQSVNQAHLFSSFSRLFVDLGIPESGDAALRAVTRKVAQAAVAA
                     LLPEPGPVHINVPFREPLEPSAIGITSPRPAVAAAPQFVAPRSALLDTGTVKSIAQLV
                     RSKPRGLIVAGPAPIGQSACRDVIGDLAWRTGFPLLAETASQLRFTGTRMDHRVDAFD
                     LILRSPRARARLQPELVIQLGAPPVSKSLARMLDEAPEIRRIVIAPFGWNDPQNTASI
                     HVRADVETVCRQISAELTVPAGTSSWAEEWSRVGEAVWQFLERGFAEAETCLSEATVA
                     RFTAGSLREDDILVVSNSLPIRDLDWFCHGESSCFRMVSQRGAAGIDGSISMALGVAS
                     AHTSRTTLLVGDSAFLHDVGALNIAAAAR"
BASE COUNT          593 a          944 c          940 g          660 t
ORIGIN      
        1 tttcctgcgt ctggcagccc tctatggcgc gttctgaaga aaggctggat gtatgttgcc
       61 gttccgttcc tggttcggcc tttcaccgac gcacaggcct atcgttacct tgctgagagt
      121 atcgaagccc actgcagtgc cgaagcgctc gtggaccttc tcacttcttg cggcgcggaa
      181 tcaccttcga ttcgattcct cagctggtgc ggcagcagtt atatcagcgt ccaagtcaac
      241 agaggccggc cacggccgcc cgagctctga gcaggatgaa tgattggccc aggttcttcc
      301 cggaaaccct gcctccaaga cacgatcgtc tcggatgagg cgttctcgtt tctgcgtgaa
      361 caactgagcg cacctatcgg aagaaactgc ttccgagcaa tcaccgttcc gattgagaga
      421 acatcgctca ccggcgccct gaaatttgac gggacagaga catggattta ctgggcgacg
      481 ccgtcgggtg aggaaaccct cgcgtggggc gccgcatgga aaaccgcccc gcgcgctcga
      541 gacaggtttg ctgccgcgga aagatctgtc tcaacccttt gggacaggat cgaacgacgc
      601 agttttccgg ggacgcggaa acccaacctg agggtatttt cgggttttgc attcgatccg
      661 gccgggcctc atgacggaga atgggatggc ttcccttcga actactgcgt gcttcccggt
      721 atctgcatta cctgttctcg cggctcgtgc agggccacgg tgattagcgg gcgggattcg
      781 taccgggaag cgctccatgg tttttcggac ttgttgggga gcctttcccc tacactcacc
      841 ggaacacaag aggatcgggc attcacgctg gacgccgcac acgaagccac cggcgagaat
      901 cagatcgcac aatgggttcg atccatcgct tcgatcaaag acgggataac gtcgggaaga
      961 ttccagaagg tggtagctgc cagaagcgtt ctgcattcgt tgtccagaac gccggacatc
     1021 aggaaagcgc ttgacctgct cgcactgcgt tacccagatt cctatcgttt cgcagctttt
     1081 ctcgggaaga agctgttcct ggcggcaaca ccagaactgc tcgtccacaa gagagcgtcg
     1141 cggttcacaa ctcatgcgct tgcgggttcg atgccgttcg gcgcgaacgc tcaggcgaaa
     1201 tacgcggccc gtcgcgctct attgcgcagc gccaaagact cagatgagca ctctctcgta
     1261 gtagaagcaa tccggcgaag cgtgggagga tttgcgaaaa cgcttgaagt ctcgaagtcc
     1321 ccgcgaatca tcctgtacgg tgatattgca catttggaga cacccttgga aggagtgctt
     1381 gagcacgatg ttcatgtcct ccgtctcgtg agtgccatcc acccgactcc cgccgtggga
     1441 ggcgttccct ccgacgaggc attggaatgg atacgcgtaa atgaggaagt gccccgggga
     1501 tggtacgcgg gaccaatcgg gtggtttgac gaaaacggtg acggctgttt cgtcactgca
     1561 atccgctcgg cccttgtggc gcccggttca gcccgtctgt tcgctggcgc tggaatcgtt
     1621 gcggattccg atccggtgac ggaatacgaa gagaccgagg cgaaactcaa accgatgaaa
     1681 cttgccctgg gcctcagcaa tgacgttcgg tgacattcag ttcctgtggg cacgcggcat
     1741 tgtggaaacc cttctgcagg caggcgtacg gtgcttcgtg gtgagtccgg ggtcgcgatc
     1801 cactccgctt gttctcgccc ttgccacaac gcgcgacatc gttgttcaca aaatactcga
     1861 tgagcgctct gcgggattct tcgcggtcgg gtatgcgaag gccaccgggc agacggtctg
     1921 tcttgtatgc acgtcgggaa gcgcccccgc acactatctg ccagcgatcg tggaagctgc
     1981 gcattccttc acgccgctgc tggttctgag cgccgaccgg ccacctgaac ttcacggcaa
     2041 cgcggcgccg cagtcagtca accaggcgca tctcttttcc tcgttctcgc gcctttttgt
     2101 cgatctaggg attccagaat cgggcgacgc ggccctccgt gcggtaacga ggaaggttgc
     2161 ccaggcggcg gttgccgcgc ttctgccgga accgggcccc gtccatatca acgttccgtt
     2221 tcgggaaccg ctggaacctt cggcgatagg aatcacgagc cccagaccgg cagttgcggc
     2281 agctccgcag tttgtcgcgc cccgctccgc ccttctcgac accggaacgg tgaaatccat
     2341 tgcgcaactc gtccgaagca aaccgcgggg actcatagtc gccggtcccg ctccaatcgg
     2401 gcaaagcgcg tgccgggatg taatcgggga cctggcttgg cgcacgggct ttccgcttct
     2461 ggccgaaaca gccagccagt tgcgcttcac cggaacgcgt atggatcatc gcgttgacgc
     2521 gttcgacctc attctgcgct cccctcgcgc acgcgcacgc cttcaaccgg agcttgtcat
     2581 tcaactcggc gctccacccg tctccaaatc tttggcgcgc atgctggacg aagcgccgga
     2641 aatccggcgg attgtgatcg ccccgtttgg ctggaatgac ccgcagaaca cggcttcaat
     2701 ccatgttcgg gccgacgtcg aaacggtgtg ccggcagatc tccgcggagt taacggtgcc
     2761 cgctggtacg tcgtcatggg ccgaagagtg gtcgcgcgtt ggtgaagcag tgtggcagtt
     2821 tctggaaaga ggattcgcag aagccgaaac gtgcctttca gaggcgacgg ttgcccggtt
     2881 caccgcgggg agtcttcgcg aggatgatat cctcgtcgtc agtaacagcc tgcccatacg
     2941 cgatctcgac tggttctgcc acggcgagtc gtcctgtttc cgtatggtga gtcagagggg
     3001 tgccgccggc atagacgggt ccatttcgat ggctctgggt gtcgcgtctg cgcacacatc
     3061 acgaacaacg cttctcgtcg gagacagcgc cttcctgcac gacgtcggcg cgttgaacat
     3121 cgctgcggcg gcgcgcg
//
LOCUS       DAOTNG010000346         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24417_length_3125_cov_5.043648, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000346 DAOTNG010000000
VERSION     DAOTNG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24417_length_3125_cov_5.043648"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="P5179_12940"
     CDS             <1..1737
                     /locus_tag="P5179_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRS96166.1"
                     /translation="VRCQSYVYGPEYYDAADEAGVLVQSEMGILGAWGGHTPWHVYQW
                     PKPTPDHYPTLKRQWDLVVQRDVNHPSANLYCMSNEYGKDTQFHGIAWQCYHDTKAIK
                     PTAMVIWTDGGFNPALPGDFVNDDAARVKDTDLPVIQHEFRWWSSLPDPAIRHKYSGA
                     VRPYAIEIAEREAARNGLSRVLAPAARNSQRLQLLEAKAKMEGCRRDNPKLAGISHFN
                     AMDANPSPQGVIDEFYERKLATPTEWQRTNGDTVIMCSLGFDQHVLVAGSTVEIAFSV
                     SDFSHPPFADPRLEWRLEVPGMQKAGAVVPYAHVPFVTNSAKSVEISVPRVQKPQRGR
                     LYAVLSDGKRSADNEWDIWVFPETQMVPENVCRHRSATVSWVGTVEVPVCDAREVSPE
                     RIVLSDVLDDSLCRYLGDGGSVILGATEGLVRPHGPNFGYVMYFFTPPANYGPYEDGQ
                     NATIVAKHPMLEGFPHDGYADFQFFRMIEEYPPLDIGGLGLPETEPVIRVFHRYPVCH
                     SLAYIVEYRVGKGTLVLCALGLNQALPEARYVLSRMCAYLARARKRKAPALSQACIDR
                     LKRATALVESGR"
     gene            1751..>3125
                     /locus_tag="P5179_12945"
     CDS             1751..>3125
                     /locus_tag="P5179_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS96167.1"
                     /translation="MARKRPNIIMITPDEWRADALGCAGNPVVKTPCMDALAKSGVLF
                     RNAYTTAPMCVPARMSILTGQSPRVHGAMTNGLSPTDNVSIAAVLRENGYRTSAFGKM
                     HFLPAYCDLGFDVLQLSEQDGEGWRIDDYHRWLWETHGLVDWIDLWDQVEDYRNEAPS
                     WFRGTYGALKSPIPEEAYHTTWITDRFLEYLDGLDGAQPFFSWIGYIKPHHPFDPPGR
                     YAELYDPSQMPLPEPDDLSTKPLVASFDPREGRLDISRWSDAEIRNMIALYYGTITQI
                     DDNVGRIVEGLHRKGIAEETVILINSDHGDFLGNRRFITKAPFVPYEDLIRVPFIVYD
                     PLNPANGRVSDAFVSLLDLFPTIAAMSGVSHNHTVHGHDLTPLLRAEAESVTDVAFAE
                     NHLGVLAIRQGPWKLLQSEHGVCELYNLDDDPGERRNRFPEMQGSRIVAGLRDAALRE
                     LMGYRWDR"
BASE COUNT          654 a          906 c          947 g          618 t
ORIGIN      
        1 gtgaggtgtc agtcctacgt gtatggaccc gagtattacg acgccgcgga cgaggcgggc
       61 gttctcgtgc agagcgagat gggcatactg ggcgcgtggg gcggccatac cccgtggcac
      121 gtctaccagt ggccaaaacc gacgccagac cactacccga cgctcaaacg ccagtgggat
      181 cttgtggttc agcgcgacgt gaaccacccg tcggccaacc tctactgcat gagcaacgag
      241 tacggcaaag acacgcaatt ccacggcatt gcctggcagt gttaccacga cacaaaggcc
      301 atcaaaccaa ctgccatggt gatttggacc gacgggggct tcaaccccgc actccccggc
      361 gatttcgtca acgacgatgc cgcgcgcgtg aaagacaccg acctcccggt cattcagcac
      421 gaattccgct ggtggagttc ccttcctgat ccagcgatca ggcacaaata ctcgggtgcg
      481 gttcgcccgt acgcaatcga aatcgcggaa cgcgaggccg cccgaaacgg tctcagccgt
      541 gttttggctc cggcggcccg taacagccag cggctacaac tgctcgaagc gaaagcgaag
      601 atggaggggt gcaggcggga taacccgaaa ctcgcgggca tcagccactt caacgccatg
      661 gacgccaacc catcgccgca gggcgtgatt gacgagttct acgaacggaa actggccact
      721 ccgaccgagt ggcagagaac gaatggtgat accgtcatca tgtgcagcct gggatttgac
      781 cagcatgtgc tggttgcggg cagcacagtc gagatcgcgt tctcggtttc tgatttctca
      841 cacccgccct ttgcggatcc gaggctcgaa tggcggttgg aagttcccgg gatgcagaag
      901 gcaggggccg ttgttccgta cgcgcacgtg cccttcgtta caaactccgc caaatcggtg
      961 gagatttcgg tgccgcgcgt acagaagccg cagcgcgggc gcctgtatgc ggtcctcagc
     1021 gacgggaaac gttccgcaga caacgaatgg gatatctggg tgtttccgga aactcaaatg
     1081 gtgccggaga acgtgtgtcg ccatcgcagt gcgacagtct catgggtggg gacggtggaa
     1141 gttccggtgt gcgacgcgag ggaggtcagc ccggagagaa tcgttttgag cgatgtgctg
     1201 gatgactctc tgtgtcgata ccttggcgat ggcgggagtg tcatcctcgg agccaccgaa
     1261 ggacttgtgc gaccacacgg cccgaacttc gggtatgtga tgtacttttt caccccgccc
     1321 gcgaattacg gcccgtacga ggacggtcag aacgcaacca ttgtcgcaaa acatccgatg
     1381 ctcgaaggct ttcctcatga cgggtatgca gatttccagt tcttccgaat gattgaggag
     1441 taccctccgt tggacatcgg aggactgggt ctccctgaaa cagaaccggt gatccgtgta
     1501 ttccaccgtt accccgtgtg tcattccctt gcgtatatcg tggaataccg cgtggggaag
     1561 ggcaccctgg tcctctgcgc gctcggtttg aaccaggcgt taccggaagc acgttacgtg
     1621 ctttcgcgca tgtgcgcgta tctggcaagg gcgaggaaac gaaaggctcc tgcgttatcg
     1681 caggcctgca tcgaccggct caaaagggcc actgcactgg tcgagagcgg ccgctgagag
     1741 gtttgtgtga atggcacgaa aacgccccaa catcatcatg atcaccccgg atgaatggcg
     1801 tgcggacgca ctcgggtgcg cgggcaatcc agtcgtcaaa acgccctgca tggacgcgct
     1861 ggcgaagtcg ggtgttctgt tccggaacgc gtacaccacg gcgccaatgt gcgttcccgc
     1921 acggatgtcc atccttaccg gacagagccc cagggtgcac ggagcgatga caaacgggct
     1981 ttcgccgacg gataacgtct ccattgccgc agttcttcgg gaaaacgggt atcggaccag
     2041 cgccttcggg aaaatgcact tccttccggc ctattgtgat ctcggcttcg acgtgcttca
     2101 gctctcggag caggacggcg aagggtggag gattgacgat tatcaccggt ggctgtggga
     2161 gacgcacggc cttgtggact ggatagacct gtgggatcag gtggaagact accggaacga
     2221 ggctccctcg tggttccgcg gtacatacgg ggcgctcaaa tcccccattc cggaagaggc
     2281 gtaccacacc acatggatta ctgaccgttt cctcgagtat ctcgacggat tggatggtgc
     2341 acaaccgttc ttctcgtgga tagggtacat caagccgcac cacccgtttg acccgcccgg
     2401 ccgctatgcc gaattgtatg acccatccca aatgccgctt cccgagccgg acgatctctc
     2461 cacaaaaccg ctggttgcca gttttgaccc gcgagagggg cgtctcgaca taagccggtg
     2521 gagcgatgca gaaatccgta acatgatcgc gctgtactac gggacgatta cccagatcga
     2581 cgacaacgtg ggtcgcattg tggagggtct gcaccgaaag gggatcgccg aagagaccgt
     2641 gatactcatc aactccgatc atggcgattt cctcggcaac cgccgcttca ttacaaaagc
     2701 tccgtttgtc ccgtacgaag acctgatacg ggttcccttc atcgtgtacg accccctgaa
     2761 tccggcaaac gggcgcgtgt cagacgcgtt cgtcagtctg ctggacttgt ttccgacgat
     2821 agcggcgatg tcgggtgtct cccacaacca caccgtgcat ggccacgatt tgaccccgtt
     2881 gctgcgagct gaggcggaat cggttactga tgtcgccttt gccgaaaatc acttgggtgt
     2941 tctggcgatc cggcagggcc cgtggaaact cctccagtcg gaacacgggg tgtgcgagct
     3001 atacaacctg gatgacgacc ccggcgagcg ccgtaacagg ttcccggaaa tgcagggttc
     3061 aaggatagta gccggattgc gtgatgctgc ccttcgcgag ctcatgggct accgttggga
     3121 caggt
//
LOCUS       DAOTNG010000347         3118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24489_length_3118_cov_4.741103, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000347 DAOTNG010000000
VERSION     DAOTNG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24489_length_3118_cov_4.741103"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="P5179_12950"
     CDS             <1..298
                     /locus_tag="P5179_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRS96168.1"
                     /translation="FVAAQCGFSGSSRIGDGVQMGGQAGVAGHISIAAGARVGAKSGV
                     MKSVSGTVAGHPARQLIQQRRAEARLMHLEELVVRVSALEKALSERPCKNHLED"
     gene            298..1197
                     /locus_tag="P5179_12955"
     CDS             298..1197
                     /locus_tag="P5179_12955"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRS96169.1"
                     /translation="MRHTVGRCVTVRGRGIHSGTDVTVRLLPADRPGITFVRSDLPGN
                     PRIPAITSNLASSSRCTRLEQGDGAVMTPEHLLAACWGTGITDLVVELDSEELPIGDG
                     SAEIWCEAFRLAGTQETPGASVALGPLPPIHVAHAGACITLTPSKTWRVEYSGRFFDG
                     PEQHVEWTEETDFRQHVARARTFCTLSDALKLRKLGMIKGGTLENALLWVDDVSLLSD
                     PALPPWVEDQPRPEKGLVRGQTLRYFDEFSRHKLLDLLGDLRLAGDLSPCAIHARCSG
                     HELNHELLREIEKHTHSQKELER"
     gene            1194..1673
                     /gene="fabZ"
                     /locus_tag="P5179_12960"
     CDS             1194..1673
                     /gene="fabZ"
                     /locus_tag="P5179_12960"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRS96170.1"
                     /translation="MSATADEPTVLDIRDIMDRIPHRYPFLLVDRIVSIREDGITGLK
                     NVTINEPYFAGHWPSEPVMPGVLQLEALAQTGAVLLFHRLGVDRQKTKLDVFFRGIDG
                     AKFRRIVRPGDQLLLDITVLKQRRELFVLKGEARVNGEVASEAELTAVLRVRGEEQP"
     gene            1670..2455
                     /gene="lpxA"
                     /locus_tag="P5179_12965"
     CDS             1670..2455
                     /gene="lpxA"
                     /locus_tag="P5179_12965"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRS96171.1"
                     /translation="MNHVDTRAVVEENAQLGSGVTVAPFAIVKAGAIANDGCTIGSYA
                     YVASGVRLGARVQVFSHACLGTIPQTKPQPPHEGFLIVGDDTVIREFTTLNSGSVLQD
                     RGGDGTTRIGARCLLMAYVHVGHDCTVGNDVILVNGATLGGHVVVEDFATISALVPVH
                     QFVRIGAYSYVAGGFRAVQDVPPYILAAGEPLRPYGLNVVGLRRHGFTAEQFRRLRAI
                     YRLFFRSGLNTAQALSRSSELEPSVERDRFVAFVERSQRGVIR"
     gene            2457..2999
                     /locus_tag="P5179_12970"
     CDS             2457..2999
                     /locus_tag="P5179_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS96172.1"
                     /translation="MARLAALGRFLFRHRDVLPVPLAVAVALSSRIRGGRLVSGALLV
                     LTGLAIRLWAVLYIGEQSRSRGDGPAFRTIGGPYAYFRHPLYLANGVLSEGLVLFSGA
                     GKRWLPFVFPVLAFLFYVPIVAWEQGRVYRRGIPVMAHRFGVRTALCSERRTYQSVLA
                     FLLVSVVSSFVRNNFRRNRQ"
BASE COUNT          586 a          902 c          974 g          656 t
ORIGIN      
        1 cttcgtggcg gctcagtgcg ggttttcggg cagctcacgc atcggggacg gcgtacaaat
       61 gggaggacag gccggcgtgg ccggacacat ttccatcgcc gcgggcgccc gcgtaggcgc
      121 caaatccggg gtcatgaaat ccgtgtcagg caccgttgcg gggcatccag cccgccaatt
      181 gatccagcag cgccgcgcgg aggcacgcct gatgcatctc gaagaactgg ttgtccgcgt
      241 ctctgcgctg gaaaaagccc tctccgagcg tccctgcaag aaccatctcg aagactgatg
      301 cgacacacag tcggcagatg cgtcaccgta cgcggacgcg ggatccacag cggcacagac
      361 gtgaccgtac gtctccttcc ggctgatcgg cccggaatca ccttcgtccg ttcggacctt
      421 cccggaaacc cgcgcattcc cgcgataacg tcgaacctcg cttcgtcctc ccggtgcacg
      481 aggctggaac agggcgatgg ggcggtgatg acgccggagc acctgcttgc tgcatgctgg
      541 ggtaccggca tcactgatct cgtcgtcgaa ctggattcgg aagagcttcc gatcggcgac
      601 gggagcgcgg aaatctggtg tgaggcgttt cgtcttgcgg gaacgcagga aacaccgggt
      661 gcctccgttg ccctgggtcc cctgcccccc attcacgtcg cccacgcggg cgcatgcatc
      721 acgctgactc cgtcgaagac atggcgcgtg gagtactccg gccgcttctt cgacggccct
      781 gaacaacacg tggaatggac agaggagacg gacttccgcc agcatgtggc acgggcccgc
      841 actttctgta ccttgagcga tgcgctgaaa cttcggaagc tcgggatgat caaaggtgga
      901 actctggaaa acgcgcttct gtgggttgac gacgtgtcct tgctctccga tcccgcgctg
      961 cccccctggg tggaagacca gccgcggccg gaaaaagggt tggtgagagg acaaacgctg
     1021 agatattttg atgagttttc gcgacacaag ctgctggacc ttctgggtga tctcaggttg
     1081 gcaggagatc tttccccgtg tgcgatacat gcgcggtgca gcgggcacga actgaatcac
     1141 gagctgctgc gcgaaatcga aaaacacacg cattcgcaaa aggagttgga acgatgagcg
     1201 ccaccgctga tgaacccaca gtgctcgata ttcgcgacat aatggaccgg attccgcacc
     1261 ggtacccctt tcttctggta gacaggatcg tcagtatccg ggaagacggt atcacgggcc
     1321 tcaagaacgt cacgatcaat gaaccgtatt ttgccggaca ctggccatcg gaacccgtaa
     1381 tgccaggtgt gcttcagttg gaggcgctcg cccagactgg agcagtgctg ctgttccaca
     1441 gacttggcgt ggatcgtcaa aagacgaaac tcgacgtctt ctttcgcgga atagatggcg
     1501 cgaaattccg ccgcatcgtc aggccgggag accaattgct tcttgacatc actgtgttga
     1561 aacaacggcg cgagcttttc gtgctaaaag gggaggcgcg ggtcaacggg gaagtcgcgt
     1621 cggaggctga actgactgcg gttctgcgtg ttcgagggga ggagcagccg tgaaccacgt
     1681 ggatacccgc gccgttgtgg aggaaaacgc acaactgggt tcgggtgtca cggtggcccc
     1741 gtttgccatt gtcaaggccg gtgctattgc gaatgatggt tgcacgatcg gttcatacgc
     1801 gtatgtcgcg tcaggcgttc ggttgggcgc gagggttcag gtgttctccc acgcctgcct
     1861 cggcacgatt ccccagacga agccccaacc gccgcatgaa gggttcctga tcgtaggtga
     1921 tgataccgtc attcgggagt tcactaccct caacagcggc tcggttctcc aggaccgagg
     1981 aggcgatgga accacacgaa tcggcgcaag atgtcttctc atggcatacg ttcatgtggg
     2041 ccatgactgc accgtgggaa acgacgtcat ccttgttaac ggagccacgc ttggcggcca
     2101 tgtcgtagtg gaggatttcg ccacgataag cgcgctcgtt cccgtacacc agtttgttcg
     2161 gataggcgcc tacagttacg tagcgggtgg cttccgcgca gtgcaggatg ttccgcccta
     2221 cattctggcc gccggggaac cgctccgccc ttacgggttg aacgtggtcg ggctgaggag
     2281 acacgggttc accgcagagc agtttcgccg gttgcgggca atatatcggc tgtttttccg
     2341 ctccggtctt aacaccgcac aggccctgag ccgctccagc gaactcgagc ccagcgtgga
     2401 gcgcgaccgt tttgttgcgt tcgtggaaag gtcacagaga ggcgtcatcc ggtaaaatgg
     2461 ccagactggc ggctcttggt agattccttt tcaggcacag ggacgtgctc cccgttcctc
     2521 ttgcggttgc ggtggcactc tcctccagaa tcagaggagg tcggctcgtt tcaggtgcgc
     2581 tgttggtgtt gaccggcctt gccatcagac tgtgggcagt tctgtacatc ggagagcaat
     2641 ctcgatcccg cggggatggg ccggcgttcc ggaccatcgg cggcccttac gcgtatttcc
     2701 gccaccccct ctacctggca aatggagtac tctctgaagg gcttgtcctg ttttcgggag
     2761 cgggaaaacg atggttgccg tttgtgtttc cggtccttgc gttccttttc tacgtgccca
     2821 ttgtcgcgtg ggaacagggg agagtgtacc gccgcggaat ccctgtcatg gcccaccggt
     2881 tcggcgttcg aaccgcgcta tgcagcgaac ggcgcacgta ccagtctgtg ctcgcatttc
     2941 tgcttgtttc ggtggtatca agtttcgtac ggaacaactt tcgacggaac aggcaatgaa
     3001 cttctggatc tcagctcttg agccttccgg cgaccggtgg ggtgcgaccc tcgtacgcgc
     3061 cattgccggt ctggctccga acgccagggt tgggggaatc tcggggcctc ttatgcgt
//
LOCUS       DAOTNG010000348         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24555_length_3112_cov_4.192673, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000348 DAOTNG010000000
VERSION     DAOTNG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24555_length_3112_cov_4.192673"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..772
                     /locus_tag="P5179_12975"
     CDS             254..772
                     /locus_tag="P5179_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96173.1"
                     /translation="MRCRNISKYYQRRAGAKVLLVSQAPGHVGARWSGIPLTDTETIA
                     SGRLGEDYEFSLVRTEPSSQAVHEGLGRHWGDVLVWSVVPWHPAGRCLDSNRKPSQIE
                     VCAGIEVLEYVLAHGEFRAIAALGEVASGALERLRVRHRVLPHPFYAGRSACIKGVRA
                     FVGDVFGSTAPS"
     gene            786..1202
                     /locus_tag="P5179_12980"
     CDS             786..1202
                     /locus_tag="P5179_12980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRS96174.1"
                     /translation="MYDDAVFVQERTVRIGDTDAAGIAYFASSLRLCHECYEALLEAR
                     NIGLREHFAPDGLALPIVHVEGDYKAPLRMGDRIRVMVTRCSLGNRSYTIEYAIVKES
                     GEIALTAKTVHVAVMLPDMKATAIPEALREALSKTG"
     gene            complement(1205..2575)
                     /locus_tag="P5179_12985"
     CDS             complement(1205..2575)
                     /locus_tag="P5179_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRS96175.1"
                     /translation="MRLSIVEAARECPTRTALYAEGTRYTFAELSERTQQRMREFTAV
                     LPKSVAGSSVAVIGSNDLETTVSLFALWELEIPALLIHPRAPLAERTRLLEVFQPAAS
                     VRGREVHPSGPFARDIPEGTACLILTSGTTGHPKAAVLSHAAFVASAEASATRLQWRS
                     DDRWLVSLSIAHVGGLSIITRCLLARSCVVLHRRFTEDEFVRAVEEDRVTQVSLVPTM
                     LERVLERYPQWRAPGYLRFALIGGGPANPDLTIRAARRGIRTLLSYGLTECCSQVCTQ
                     PLDRSDDDPTCGEPLDGFQVETRDSEIWVKGPAMMTGYYPQHGAPVFDKDGWFGTGDA
                     GVLEDGQRLRVLGRLDDAIVTGGEKVHPSEVEAILLTCPNVRAACVFGRQDAVWGQII
                     CAALTPAPGTSVDSEDLRTTCARYLSPSRMPRKYCILDHIPLNRNGKHSRAETARVAT
                     PLLRDL"
     gene            complement(2556..>3112)
                     /locus_tag="P5179_12990"
     CDS             complement(2556..>3112)
                     /locus_tag="P5179_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRS96176.1"
                     /translation="LKKLADWGVEYCEQPVPRGELRMLSPSPVPLAADESLTDMKELE
                     AFLGIPAKRINEFLRGLVTPEDVLKAAPEGFSPPVKVFVLKPMVLGGILRSRRIARIA
                     AERGIRYTVTHLFDGATAFAAYAEFAVSLQPPPLACGLFPHDATAAAGTVDDSHFDGS
                     CVRSAHQPGLGVNRGAILHAALDR"
BASE COUNT          603 a          897 c         1006 g          606 t
ORIGIN      
        1 agagcggaaa ggcgaactca agggtgctgc aagctggggc tgatacattg ccgcacacgg
       61 cacctgaggc gatcagcatt gttttggtgg cagagaacaa gctgtgggcg atttgatacg
      121 gggggcgacc cctgtgccgg tgtcggatac ctgcgcgcaa ggtctgctcc agcgcttgca
      181 ccttgcacct tcgactttga acaatcagta cacggtaacg tacgccgaag aggctccgct
      241 tgagggggcc gggatgcgct gccggaacat ctcgaagtat tatcagcggc gagcgggtgc
      301 gaaggtactc ctcgtgagcc aggcgcccgg gcatgtgggg gcccggtggt cgggtattcc
      361 gctcacggac accgaaacta tcgcgtcagg ccgcctgggt gaagactacg agttcagcct
      421 cgttcgtacc gagccctcct ctcaggcagt gcatgaaggg ctgggcagac actgggggga
      481 cgtgcttgtc tggtcggtcg taccctggca tcccgccggc cggtgcctgg actccaaccg
      541 gaaaccgtcg cagatcgagg tttgcgcggg aatcgaagtt ctggaatacg tgctcgcaca
      601 tggtgagttc cgggctatcg cggcgcttgg agaggtagcg tcaggagcac tggaaaggct
      661 ccgtgtacgt caccgtgttt tgccgcatcc cttttacgcg ggccggagtg cgtgcatcaa
      721 aggggtacgg gcgtttgtgg gggatgtgtt cggttccacc gcaccatcgt gagggacgat
      781 cagggatgta tgacgacgcg gtattcgttc aggaacggac cgtgcgcata ggcgacaccg
      841 acgcagccgg catcgcgtat ttcgcgtcgt cactgcggtt gtgtcatgag tgctacgaag
      901 ctctgctgga agccagaaac atagggctgc gggaacattt cgcaccggac ggattggccc
      961 ttccgatcgt gcacgtggaa ggagactaca aagctccttt gaggatgggc gaccggattc
     1021 gagtgatggt aacaaggtgc tcacttggaa accgttccta cacgatcgaa tacgcgatag
     1081 tcaaggaatc cggcgaaatt gctcttactg cgaaaacggt gcacgtcgcg gtcatgttgc
     1141 ccgacatgaa ggctaccgca attccggagg cgttgcgcga ggcgctctcg aaaaccggct
     1201 gagtctacag gtcgcggagg agcggagtcg cgacgcgtgc ggtttcggcg cggctgtgtt
     1261 ttccgttgcg gttgaggggg atgtggtcca ggatgcaata tttcctcggc attctggacg
     1321 gggaaagata cctcgcgcac gtcgttctta agtcttccga gtcaacactg gttcccggag
     1381 ccggtgtcag ggctgcgcat atgatttgcc cccatacggc gtcctgacga ccaaagacgc
     1441 acgctgcacg cacattcggg cacgtcagaa ggatcgcctc cacttcggaa ggatgcacct
     1501 tctcgccgcc ggtcacgatg gcgtcgtcca gtctgccaag tacgcggagg cgttgcccat
     1561 cctccaaaac gccagcgtcg ccagtaccaa accagccatc tttgtcaaac acaggtgcgc
     1621 cgtgctgtgg atagtatccg gtcatcatcg cagggccctt tacccatatc tcggagtctc
     1681 tggtttctac ctggaaacca tccagaggtt cgccgcaggt cggatcatca tcggaacggt
     1741 caaggggttg cgtgcatacc tgcgaacagc attcggtgag tccgtaggaa aggagcgttc
     1801 tgataccccg ccgggcggct cgtatggtga ggtcaggatt cgccggcccc ccgccgatga
     1861 gagcaaatcg aaggtatccg ggcgcgcgcc actgagggta acgctccagc acccgttcca
     1921 gcatcgttgg tacgagggac acctgagtca ctctatcttc ctctacggca cgcacgaact
     1981 cgtcttcggt gaagcgtcgg tgcaaaacga cacaggaccg cgcgaggagg caacgcgtga
     2041 taatggaaag gcctccgacg tgtgcaatcg agagcgagac cagccagcgg tcgtctgacc
     2101 tccactgaag ccgggtggcg ctggcctcgg cacttgcgac gaacgccgcg tgggagagca
     2161 ccgccgcttt tgggtgcccg gtcgtgccag aggtcaggat gaggcacgcg gtcccttccg
     2221 ggatgtcccg tgcgaatggt cccgaagggt gcacctcgcg ccccctcacg gacgctgccg
     2281 gttggaacac ctccagaagg cgggttctct cggcaagggg tgcgcgcggg tggatgagga
     2341 gagcggggat ttccagttcc cagagcgcaa aaagggacac cgtcgtttcg agatcattcg
     2401 agccgatcac cgccacgctg ctgccggcga cacttttcgg caggaccgcg gtgaactctc
     2461 gcatgcgttg ttgcgttcgt tcgctgagtt cggcgaacgt gtaccgcgtt ccctctgcgt
     2521 acagtgccgt acgagtcggg cactcccgcg ctgcttcaac gatcgagagc cgcatggaga
     2581 attgctccac ggttgacgcc gagcccaggt tgatgggcgc tccggacgca agacccgtcg
     2641 aaatgactgt cgtccaccgt tcccgccgcg gcggttgcgt cgtgcggaaa caggccgcac
     2701 gcaaggggcg gcggctgaag cgaaaccgcg aactcggcgt acgcggcgaa cgcggtcgct
     2761 ccgtcaaaaa ggtgcgttac tgtgtagcgg attccgcgtt ctgccgcgat gcgcgcgatc
     2821 cttcgcgatc gcagaatgcc gccaagcacc atcggtttga ggacgaacac tttcaccggt
     2881 ggcgaaaagc cttcaggcgc ggctttcagg acatcttcgg gtgtcactaa gcctcggaga
     2941 aactcattga tgcgtttcgc cgggatgccc aggaatgcct caagttcctt catgtcggtg
     3001 agcgactcat cggccgcgag aggcacgggg gatggactca gcatccgtaa ctcgcccctt
     3061 ggaacgggtt gctcgcagta ctcgactccc cagtcagcga gctttttgag aa
//
LOCUS       DAOTNG010000349         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24742_length_3092_cov_6.896279, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000349 DAOTNG010000000
VERSION     DAOTNG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24742_length_3092_cov_6.896279"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1076
                     /locus_tag="P5179_12995"
     CDS             252..1076
                     /locus_tag="P5179_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS96177.1"
                     /translation="MGMQSFRKRARKIAYFGVVAGMFMVSYAFGQAAAPAEGAAAPAA
                     GGAPTEQTIDPNIVVKKVVTGFMSDFKPKATGAWLFQWPIVISFACGIGIAAERYYTI
                     VMKSKVNMKKFTDQIIKFVEVKDYDKAIALCNASPSGALPQLVKAGLEAAKRNMGTRA
                     VQDAIDEKTLSILPKITSRTSWVSVLANVSTLLGLMGTIFGLMYTFAGLELVLNPKGK
                     ADLLTSGIATAMNTTLLGLVCAIPLTLLFQYLSQASKAIVDDIDESSVRIINALSR"
     gene            1217..1795
                     /locus_tag="P5179_13000"
     CDS             1217..1795
                     /locus_tag="P5179_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96178.1"
                     /translation="MPGFAPSKRSRAEEKETPLNLQVVMNLICILIPALLSMQVQDYY
                     RHDVELPTRGGGGGGPSSGPTNPPFNLKMTIMEDGSFVIVNAKTLSPADGLIPAGAGL
                     AVPPINGEPDYQKLQLVMMKEHKERLGGQPPEAFPDPDQITVSAPGDLDFQHVIKALD
                     RLRFEKPASELDWSSAKQMFTVISLSPGSVGG"
     gene            1826..2302
                     /locus_tag="P5179_13005"
     CDS             1826..2302
                     /locus_tag="P5179_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS96179.1"
                     /translation="MPGFPPSKRKAKEEEGGDINITSLMDAFTIILVFLIKQFGTSAV
                     EISAGYRPPVSNTRLAIDRILPLQVRQIGPDAISYQISDKPEKAERKDPNVGYAQLRA
                     DLKAQKPLVDAAVVDEELKGAINIVCDKSVTYATLMDVMKSVAGSGFFKLKLIAEP"
     gene            2338..>3092
                     /locus_tag="P5179_13010"
     CDS             2338..>3092
                     /locus_tag="P5179_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96180.1"
                     /translation="MADNHVPPPAQAKAVAAPMVFPPELRRHFYDTADWTSIIFLLVS
                     LTINMLGIGIAASREWKDPAAGLTDEQLMAMQDRIMATFKPVEVKPEEMSAAEQLENL
                     VNNPEFEQALAQQQANLAASLQSTLAEAQATLASLDAQTSAGAAGGADVQGQLDDILG
                     SLGDIGAVGAADAGGPSLDLAPTDVAIFAEGAGSGSRVVSGAIDVAALGSSAKISSAF
                     TRGGLSSGEAARLSQQIAFARERLGGNVQVRVGT"
BASE COUNT          671 a          851 c          936 g          634 t
ORIGIN      
        1 cacggacgtc ccgtcctgcc tatggagcca atcgtgtggc cgcccgaacc ggcgcagtaa
       61 cgcgcggaga agcgtcttga cgtgcggggg gtgacaggct aatttcagtt cgtcgcggta
      121 aggtgaaaag gatcttgcca gaactcgagt gtggtagcgg gcaggtgtat cagaagtcgg
      181 tcgggcgcgg tcaaacgggt cggcggctcg ccgggtgtct ttcataagtt tcagtatcta
      241 cggaggatac gatggggatg cagtccttcc gaaaacgggc gaggaaaatc gcctattttg
      301 gtgtcgtggc gggaatgttc atggtgtcgt acgcatttgg ccaggcggcg gctcctgcgg
      361 aaggcgccgc agcccctgcg gctggcggcg ccccgaccga gcagacgatt gacccgaaca
      421 tcgtcgtgaa gaaggttgtt acggggttca tgagcgattt caaacccaaa gccaccggtg
      481 cgtggttgtt ccagtggccg atcgtgattt cgtttgcgtg cggtatcggc atcgccgcgg
      541 aacgctacta cacgatcgta atgaaatcga aagtgaacat gaagaagttc accgatcaga
      601 ttatcaagtt cgtcgaagta aaggattacg acaaagcgat cgcgttgtgc aacgcatcgc
      661 cttcgggagc cctgccgcag cttgtaaagg ccggtttgga ggccgcgaaa cgcaacatgg
      721 gaacgcgcgc ggtgcaggat gcgatcgacg agaaaaccct ttcgatcctg ccgaagatca
      781 cgtcgcggac ctcctgggtg agtgttttgg caaacgtctc gacacttctg ggtcttatgg
      841 gaaccatctt cggtctgatg tatacgtttg ccggacttga actggtactg aacccgaagg
      901 gcaaggcgga ccttctgact tcaggcattg caacagctat gaacacgacg ctgctgggtc
      961 tggtgtgcgc gattccgctc accctgcttt tccagtacct gtcgcaggcc agcaaagcga
     1021 tcgtggatga cattgacgaa agctctgtgc gaattatcaa cgcgctgagc aggtgatttg
     1081 gcatctgatt tgatctctgc gtgcccggca tcctttcctc gaaaaaagga tgccgggggg
     1141 aaagacgccc acgggtaatg cgttgttccc ggagcggatc gagatttcga ccttttagac
     1201 gtgtcgagga acagcaatgc caggctttgc gccatcgaag agatcgcgtg cagaagagaa
     1261 agagacgccg ctgaatctgc aggttgttat gaaccttatc tgcattctca ttccggcgct
     1321 cctctccatg caggtccagg attactaccg gcacgacgtg gaattgccta cgcggggcgg
     1381 cggcggtggc ggcccaagtt ctggtcccac gaaccctccc ttcaacctga aaatgacgat
     1441 catggaagac gggtccttcg tgatcgtgaa cgccaagaca ctctcccctg ccgacggtct
     1501 gattccggcc ggggcgggcc tggccgttcc tcccatcaat ggcgagcccg attaccagaa
     1561 gcttcagctt gtgatgatga aagagcacaa agagcggctc ggcggccagc caccggaagc
     1621 gtttccagat cctgaccaga tcaccgtatc cgcacccggc gacctcgatt tccagcacgt
     1681 gatcaaggcg ctggatcgcc ttcggttcga aaaaccggcc agtgagcttg attggtcgag
     1741 cgcaaaacaa atgtttaccg tgataagtct ttctcccggt agcgtcggtg gttgaggaat
     1801 acctcacagc gtaaggggtt agcgaatgcc aggcttccca ccatcaaaac ggaaagcaaa
     1861 agaggaagag ggcggcgaca tcaacatcac gtcgttgatg gacgccttca ccatcatcct
     1921 cgtgttcctc atcaagcagt tcggtacgag cgccgtggag atatccgcgg ggtatcgtcc
     1981 cccggtctcc aacacccgtc tggccattga tcgcatcctg ccgcttcagg tccgtcagat
     2041 cggtccggac gcgatttctt accagatttc cgacaagccg gaaaaggcgg aacgcaagga
     2101 cccgaatgtc ggatacgctc aactacgtgc ggacctgaag gcccagaagc ctctggtgga
     2161 tgccgcggtg gttgacgaag aactcaaagg cgcaatcaat atcgtgtgtg acaaaagtgt
     2221 tacctacgcg acgttgatgg atgtgatgaa gagtgttgct ggttccggct ttttcaaact
     2281 gaaactcata gccgaaccat aaagtccggt gcaaacgttg ctttctgagg tgcaaccatg
     2341 gcagacaacc acgttcctcc accggcgcaa gcaaaagcag tggcagctcc gatggtattt
     2401 ccgcccgagc ttcgcaggca tttctacgac acggccgact ggaccagcat catcttcctg
     2461 ttggtatcgc tcaccatcaa catgctgggc attggaattg ccgcgtcgcg ggagtggaaa
     2521 gaccccgctg caggtctgac cgacgagcag ctcatggcga tgcaggatcg catcatggcg
     2581 acgttcaaac cggttgaagt gaagccggag gagatgtcgg cggcggaaca gctcgaaaac
     2641 cttgtcaata accctgagtt cgaacaggca ctcgctcaac agcaggcaaa cctcgcggca
     2701 agtcttcaga gcaccttggc cgaagcccag gcgacactcg cctcgcttga cgcccagacc
     2761 tcggctggcg ctgcaggcgg cgctgacgtg caggggcagc ttgacgacat actggggagc
     2821 ctcggcgaca ttggagcggt cggtgccgca gacgcaggtg gtccttcgct tgacctcgcc
     2881 ccgacggatg tagcgatctt cgcagagggc gctggctcgg gaagccgcgt ggtttcgggg
     2941 gcgattgacg tggcagcgct cggttcgtcg gcgaagatat cctctgcgtt tacccgcgga
     3001 ggcctttcct ccggcgaagc tgcgcgtctt tcacagcaga ttgcctttgc gcgcgaacga
     3061 ctgggtggaa atgtgcaggt gcgcgtcgga ac
//
LOCUS       DAOTNG010000350         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_24934_length_3075_cov_2.440066, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000350 DAOTNG010000000
VERSION     DAOTNG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24934_length_3075_cov_2.440066"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="P5179_13015"
     CDS             <1..353
                     /locus_tag="P5179_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96181.1"
                     /translation="NKAIARPLFEGTLMWGRDVDGKAVLVDDLSKKYANMPAAKKAQM
                     LLATGRIQEGTLKMWQGLPLAQYETLVNSRFETKERRCSAISSSEKPASRMRFSHLSD
                     AFDSTRSGSVPGSS"
     gene            complement(479..754)
                     /locus_tag="P5179_13020"
     CDS             complement(479..754)
                     /locus_tag="P5179_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96182.1"
                     /translation="MPMYSWYCRACKKTTPEYFHVKDLPETVKCRKCGRDCKQDFEAK
                     RPQAIQDSFDHPKDMSSIFGTVAHSRSEAKAIMRKYDEKYGTKLEFA"
     gene            complement(759..2642)
                     /locus_tag="P5179_13025"
     CDS             complement(759..2642)
                     /locus_tag="P5179_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96183.1"
                     /translation="MADPDSFFANDSSRGSLHIICRKLNTLTTLKNAQFAVSAKKPID
                     EPFARLGEKALDRETRRMQWQRRMRTIGMWTTLTGTGCCKLGIDSMYQYGEAAYSDKI
                     PRKSDYYDEYKDQPYGPLTEYTDPSIRVGEVNMIHIPTTNVFANPEATTRDTVTRWYI
                     RYQRPLIDALRDYRYDPQARGALRGMTTNDARYSVWLDERDDEERLWARYCEVVECFD
                     LSSRQVCVFTEYGNKPLIDWHNFRLDVFCPLHLFTPIDHPTNWRGMPYAMLIYNQHKS
                     INQLRALLKAKIGSDGKTVYAYDPDRITEEQLTPLRKARDGEWVPIEGLLSMMQNGEE
                     FFKVIEFGKPNPDILRMMQMFENDLQFMSGMDEPTRGIETGGEKTATEVSVRQQQQSL
                     TISDYVARNEEFQEEVAGDVLRLVVQNWDQQKLVKVCGSDDDSYFWVNIERERMRTEF
                     DLEVVAGSTEKMDSTTYRRQYNEMTDRLVQVAGVAMQENQLVAQGIQPTGIVWSEVIR
                     RLVNNYEPCLGNQILNKRDPFALMMDLIQQHNIWPVKMSADLEQRLVAAFASPPPMQG
                     QQPGMQPGQQPGGMPPNVVPMPGQQGTPTAVQQPQVNSMNAPQDMAGFTSGRQMSEAH
                     GGS"
     gene            complement(2796..>3075)
                     /locus_tag="P5179_13030"
     CDS             complement(2796..>3075)
                     /locus_tag="P5179_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96184.1"
                     /translation="RIRDQIQPALGARRIYVLRGMLNEERLVKEITDMTNQSKVGFDV
                     LDTLADLCSEAQAKPKEKQARQARGMYHEKQDRAVRSMVTRKSSLGGW"
BASE COUNT          572 a          870 c          879 g          754 t
ORIGIN      
        1 ataacaaggc catcgcccga ccgctctttg agggcacgct gatgtggggc agggatgtgg
       61 acggcaaggc ggtgctggtg gatgacctgt ccaaaaagta cgccaacatg ccggccgcca
      121 agaaggcgca gatgctgctg gccactggcc gcatccaaga gggcacgctg aaaatgtggc
      181 agggcctgcc gctggcccag tacgaaacgc tggtgaattc ccgtttcgag acgaaggaac
      241 ggcggtgttc cgcaatttca tcgtcagaga aaccggcttc acgcatgcgg ttttcccact
      301 tgtcggatgc gttcgactcg actcgttcag ggtccgttcc cggatcgtcc tgagtgtcga
      361 tatcgacttc ctcatcttcg taaaacatcg tgctctggct cctgcaaaaa ggtctgtccg
      421 ggtcatccgc tcgctcattc gccttggtgc gagtgcttcg cgggtccatc aagaccggtt
      481 aagcgaattc caactttgtc ccgtactttt cgtcgtactt ccgcataatt gctttggctt
      541 cggatcgcga gtgcgccacc gtgccaaaaa tcgaggacat atcttttggg tgatcaaagc
      601 tgtcctgaat ggcttgcggg cgtttcgctt cgaagtcctg tttgcaatcg cggccgcatt
      661 tccggcactt caccgtttcc gggagatcct tcacgtggaa gtactccgga gtggttttct
      721 tgcaagcgcg gcagtaccac gaatacatgg gcatggctct aacttcctcc gtgcgcttcg
      781 gacatctgac gtccggacgt gaatccggcc atgtcctgtg gcgcattcat ggaattaact
      841 tggggttgtt gcactgcggt gggcgtgcct tgctgccccg gcatcggtac gacattcggg
      901 ggcataccac cgggttgctg tcccggctgc attccgggct gttgaccctg cattggcggc
      961 ggcgaggcaa atgcggcgac gaggcgctgc tctaaatcgg cgctcatctt caccggccaa
     1021 atgttgtgtt gctgaatgag gtccatcatc agcgcaaagg gatcgcgctt gttcaggatt
     1081 tggttgccga ggcagggctc gtagttgttc acgagacggc gaatgacttc cgaccagaca
     1141 atgcccgtgg gctgaatgcc ttgcgctaca agctggtttt cctgcatggc gacaccggcc
     1201 acctggacaa gccgatctgt catttcgttg tactggcggc ggtacgtcgt ggagtccatt
     1261 ttttccgtgg acccggccac aacctctaga tcgaattcgg tgcgcatccg ttcgcgctcg
     1321 atgttcaccc agaaatagct gtcatcgtcg gacccgcaaa ccttgacgag tttttgctga
     1381 tcccagttct ggaccacgag gcgaagaacg tcgccggcaa cctcttcctg aaactcttcg
     1441 ttgcgcgcaa cgtaatccga aatagtgagg ctttgttgtt gttgtctgac cgaaacttcg
     1501 gtggccgtct tttcgccgcc cgtctcgatt ccgcgcgtgg gctcatccat cccggacatg
     1561 aattgcagat cgttttcgaa catctgcatc atgcggagaa tgtcgggatt cggcttgcca
     1621 aactcgatga ctttgaagaa ctcttcaccg ttctgcatca tcgacaggag cccttcaatc
     1681 ggcacccatt cgccatcgcg cgccttgcgc aacggggtca attgctcttc ggtgattcgg
     1741 tcgggatcgt aggcgtagac cgttttcccg tcgctgccaa tcttggcctt gagcagcgcg
     1801 cgaagctggt tgatgctctt gtgctggttg taaatcagca tcgcgtaggg catcccgcgc
     1861 cagtttgtgg ggtgatcgat tggcgtgaaa aggtgcagcg gacagaacac gtccaaccgg
     1921 aagttgtgcc agtcgatcag cggtttgttg ccgtactccg tgaacacgca cacttgccgg
     1981 ctggacaggt cgaagcattc gacgacttcg caataccgcg cccaaagccg ttcttcatcg
     2041 tcgcgctcat ccagccacac gctgtaccgg gcatcgttcg tggtcatgcc gcgcagtgcg
     2101 ccgcgggcct gtgggtcata tcgataatcg cgaagagcat cgatcaaggg acgctggtac
     2161 cggatatacc accgggtcac cgtgtcgcgc gtcgttgctt ccggattggc aaagacgttc
     2221 gtagtgggga tatgaatcat gttcacttcg cccacacgaa tcgagggatc ggtgtattcg
     2281 gtcagaggcc cgtaaggctg atctttgtat tcgtcgtaat agtccgactt gcgcgggatc
     2341 ttgtccgagt acgccgcttc cccgtattga tacatcgagt caatgccgag cttgcaacag
     2401 cctgtgccgg tgagtgtggt ccacatgccg atcgtgcgca ttcgtcgctg ccactgcata
     2461 cggcgcgttt ctcgatcgag ggctttctcg cccaggcgcg cgaaaggttc gtcaatcggt
     2521 ttcttggcgg ataccgcaaa ctgcgcgttt ttcagcgtgg tcagggtgtt cagtttccgg
     2581 cagatgatat gcaagctgcc acggctggaa tcgttcgcaa agaagctgtc cgggtccgcc
     2641 atgttttccg caacgaagtc gtagacctct tggaaactgc gcatcccgta ttcgtgaaaa
     2701 atcgcgtacc acttctgatg gtaatcgtcg atatcccggt ccaccgtgta ccggtattcc
     2761 tcttggagaa atcgaagctg gtcgagtttc atcagctacc accccccaag gctggatttg
     2821 cgcgttacca tcgaacgcac cgcgcgatcc tgtttctcgt ggtacattcc gcgggcttgc
     2881 cgggcctgtt tctctttggg ttttgcctgt gcttcagagc acagatccgc gagggtatcg
     2941 agcacgtcga aaccgacctt gctctgattc gtcatatcgg taatctcttt gacgagacgc
     3001 tcttcgttga gcatcccgcg gagcacgtag attcggcgcg cgcccaatgc cggctgaatt
     3061 tggtcgcgga tacgg
//
LOCUS       DAOTNG010000351         3053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25167_length_3053_cov_3.988993, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000351 DAOTNG010000000
VERSION     DAOTNG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25167_length_3053_cov_3.988993"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..>3053
                     /locus_tag="P5179_13035"
     CDS             326..>3053
                     /locus_tag="P5179_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRS96185.1"
                     /translation="MRNRLLNLTCLIALLALPTFAHAVVEDMSLKTITGHTGALNAVA
                     ASVSRIATGSSDGTVKLWSTNGDTLFQTLATPDGGSVFSLAFSPDGSQLAVGTGMHGV
                     WLYDVATGTVVRQFEGLTGTIMSISFSTDGRIAAGDNMGDIAVWGVVPGGVPLAVIAH
                     GYAVRALLFSADNNHLLVGGVIFNTVAIYPSTGGGPVGSFAGNGADIYSLALSPAGNV
                     LVSGGADGTIRVWDYATRVLTNTVPNVHDGMAVKCLAAFTDEVGRLLAVSGGGDNMVK
                     GWELSTGALEVAFSGHTAAVNSVALIPGQKRAVSGSADMTAKIWGDNGGQPPPQDKSI
                     ATFPHGAPVLGVAASPAGDTAATIAGELVRLWGEAGNLLTVNPDLGQLSAIAYSPDGS
                     RIAVGGTRYVQVLDAATLSNAQWFQGLQAAVKSIAFNPAGSLVAAGDSAGNLYVWNTG
                     DGSVLWRKVELGGAVNAVAFSPDGAFVVAGGGFGNYGARLFDAATGAPGNNFYGHTGP
                     ITGLAFSSSGGKLVSSSVDGSIRSWYFPTAEDAITISGAHSGTAVNCVAVKEIEAGIE
                     VIASGGDDNNLKIWDLATGQQDMDSGDLTGHTGPVVSVAFMGDWGGITGSLDMTAKIW
                     GGEGGSSGPTNVSGEIMSQTWTRQGSPYRIVGPVMVPPSETLNIDSGVEVQFVTNVPF
                     VVAGGLNINGSPMDSVWFHGFDSNWGGIRFVGMEPVAKPSYGPAPSAYLNGVIIEGGS
                     ASGETTAEQSGGGIYVGPGREVNISNSSIRGNAAGYAGGGIYVDGGSLWLYQSKVSGN
                     SAGAMGGGIAAGGGSVNLSQSGVAGNTAQTRGGGIALEEAASLYLWQANVANNNALVE
                     GGGISLLGGSSANLENAQIVRNYTNGSGGGIFGRNSSMNSWRMVVAQNTGRNS"
BASE COUNT          543 a          966 c          952 g          592 t
ORIGIN      
        1 ccgtgccgca ccgaaccgcc attcattctt cgcccatcga tcccacacga atcccactgt
       61 cctccttctt cgcgtcttcg tgcgcgcccc ctttttccac tctcccttcg cgtcttcgtg
      121 tgagcccctt tgtcttctcc cccttcttcc cctcccccgc ctcctccggt gctcacgctc
      181 tcccctggtt cggcgtatat ttgaatccac gtggcgtgcc ttgtctcgcc acgcggatgt
      241 tcaactctga tcgcacctca tgccggctta ttcggcgaaa ctgcacaaac gcgccgccca
      301 tgcggccgaa tgaacggggg tacccatgcg gaacaggtta ctgaatctta cgtgtctcat
      361 cgcattgctt gcgctaccca cttttgcgca cgcggtcgtg gaagacatgt cgctcaaaac
      421 gatcacgggg cataccggcg ccttgaacgc ggtcgcggca agtgtcagcc gcattgcgac
      481 gggcagcagt gatggcacag tgaagttgtg gagcacgaac ggcgacacgc ttttccagac
      541 gctcgcgact cctgacggcg gctcggtgtt ctcccttgcg ttcagcccgg atggttcgca
      601 actggcagtc ggcaccggaa tgcacggcgt ctggctttac gacgtcgcca caggcacggt
      661 tgtgcgccag ttcgagggcc tgaccgggac tatcatgagc atctccttca gcactgacgg
      721 cagaatcgcc gccggtgaca acatgggcga catcgccgtc tggggagtgg ttcccggcgg
      781 cgtgccattg gcagtcatcg ctcacgggta tgcagtacgc gcgctgttgt tcagtgccga
      841 caacaaccac ctgctcgtgg gcggcgtgat tttcaacact gtcgccatat acccctccac
      901 gggtggaggc ccggtcgggt cctttgcggg caacggggcg gacatctaca gcctcgcgtt
      961 gagccccgcc ggcaacgttc ttgtatcggg cggcgctgac ggcaccatcc gcgtgtggga
     1021 ttacgccact cgcgttctta ccaacaccgt tcccaacgtt cacgatggaa tggccgtaaa
     1081 gtgcctggcg gcgtttaccg acgaagtggg tcgcctgctt gccgtatccg gtggtggcga
     1141 caacatggtc aaaggctggg agctttccac aggcgctctg gaagtggcgt tttcgggcca
     1201 taccgccgca gtgaacagcg tcgcccttat ccccggccag aaacgcgccg tctccggcag
     1261 tgccgatatg acggccaaaa tctggggcga caacggtggt cagccgccgc cccaggataa
     1321 gtccatcgcc accttcccgc acggcgcccc cgtgctcggc gttgcggcga gccccgcagg
     1381 ggatactgcc gccacaatcg ccggcgaact cgtccgtctc tggggcgaag cgggcaatct
     1441 cctcacggtg aaccccgatc tggggcagtt gtccgccatc gcctacagcc ctgacggctc
     1501 cagaatcgcc gtgggcggca ccaggtatgt gcaggttctc gatgcagcca ccctttcaaa
     1561 tgcacagtgg ttccaggggc tgcaggctgc agtcaaaagc atcgcgttca acccggcggg
     1621 ctctctggtc gctgcgggag attccgcggg caacctgtat gtgtggaaca ccggggatgg
     1681 atccgtcctg tggcgcaaag tggagttggg tggcgcggtg aatgccgtgg cgttcagccc
     1741 cgacggggcg tttgtcgtcg cgggcggtgg attcggaaac tacggcgcga ggctttttga
     1801 cgcggcaacg ggtgcaccgg ggaacaactt ttacggccac acgggcccga tcacgggcct
     1861 tgcgttttcg tcatccggcg gcaaactcgt gtcgtcgagc gtggacggat caatcagaag
     1921 ctggtacttc cccaccgccg aagacgcgat cacgatctcc ggagcacact ccggcaccgc
     1981 cgtcaactgc gttgcggtga aggaaattga ggcggggatc gaggtgatcg cctcgggcgg
     2041 cgacgacaac aacctgaaaa tctgggacct cgccaccggc cagcaggata tggattcggg
     2101 tgatctcacc ggccacaccg gccccgtcgt gagtgttgcg ttcatgggcg actggggcgg
     2161 catcacgggc agcctcgaca tgaccgccaa gatttggggc ggcgagggtg gttcatccgg
     2221 cccgacgaac gtctcaggtg agatcatgtc acaaacgtgg acacggcagg gcagcccgta
     2281 ccgcatcgtc ggcccggtga tggttccccc ttcggagaca ctcaacatcg actccggcgt
     2341 cgaagtgcag ttcgtcacca acgttccctt cgtcgtcgcc ggtggtttga acattaacgg
     2401 ctcgccgatg gacagcgtgt ggttccacgg gtttgacagc aactggggcg gcatccggtt
     2461 cgtcgggatg gaaccggttg cgaaaccctc gtacggacca gcgccgtcgg cgtacctcaa
     2521 cggcgtcatc attgagggtg gctcggcctc cggcgaaacc accgcggaac agagcggcgg
     2581 cggaatctac gtcggtcccg gccgagaggt gaacatctcc aattcgtcta tccgcggaaa
     2641 cgccgccggg tacgcgggcg gcggcatcta cgttgatggt ggatcgctct ggttgtatca
     2701 gtccaaagtg agcgggaaca gcgcaggagc catgggcgga ggcatcgccg caggcggtgg
     2761 ttccgttaac ctctctcaat caggcgtcgc cggtaacact gcgcaaactc gcggcggtgg
     2821 catcgccctg gaagaggcag ccagcctgta tctctggcag gcaaacgtcg ccaacaacaa
     2881 cgcactggtg gaaggcgggg gtatttcgct cctcggcggg tcgagcgcca atctggaaaa
     2941 cgcgcagatc gtccggaact acacgaacgg ttccggcggt ggcattttcg gccggaattc
     3001 ctcgatgaac tcctggcgca tggtagtagc gcagaatacc ggcaggaatt cgg
//
LOCUS       DAOTNG010000352         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25184_length_3052_cov_3.829496, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000352 DAOTNG010000000
VERSION     DAOTNG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25184_length_3052_cov_3.829496"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1141
                     /locus_tag="P5179_13040"
     CDS             221..1141
                     /locus_tag="P5179_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96186.1"
                     /translation="MTTLHNQSFFWVRGFGYQPSYAPTGLEIWLHFDERLIATELARA
                     NEHFPGTNAIRLWLSFDAWLREPARFVRNFGRVLDILGHLGLRAMPCLFNNWRSLPDF
                     GGINAEMVGYWNSPHREVNYFHRYARDLVTPFLNDERILVWDLCNEPFNSGREEVFVP
                     WLADLHGFVKGTGAVAPVTIGVPPDLHQLELVEPMCDVLTPHLYGASAANDAFIEFAN
                     RVAKPMFAGETGWGSLDDDVRLETLQGELSSLLERRVGFMIHVLHHSLVADCHRAEFG
                     PVSEVGFMGCIEADGTMRAGYESIRKYFAQ"
     gene            1155..2204
                     /locus_tag="P5179_13045"
     CDS             1155..2204
                     /locus_tag="P5179_13045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS96187.1"
                     /translation="MSELHHTDVFVSGQDGFAFYRIPVIEVAADGSILAFVEARKHNL
                     GDPGQDKNHIALAMKRSTDGGRTWSPMVIIEDPGEGWSAANPATVFDRDTGRVWLHYL
                     RSKPGRGSEHARPGTDDLQNLVRTSDDNGVSWSEPTDITTVCRDMADPKWRCTVVGPG
                     GGIQDSKGRLMVACWMTEPMRVFAVFSDDHGHTWQRGGFVPGGILGNEDQLVELADGR
                     ILLDFRQAEGDHRWFAESTDGGRTWSQPRVGEPVSPVACAIERFTLKSAGDDKNRIIW
                     SGPKGPGRENLVVRISEDEAASFPNELLVATGKAAYVDAARLKDGSIGLLWEREHYQF
                     ITFTTLDLAFLTSTG"
     gene            2227..>3052
                     /locus_tag="P5179_13050"
     CDS             2227..>3052
                     /locus_tag="P5179_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96188.1"
                     /translation="MTETELLGQLRGIENRVPCGGAGTPRDVTGYASLARRRTWTQRW
                     DADARTSREPHEVTGTVWSDAIERCLAETGSVLIPRMEAPVYLDRPIVLGSGARLIVH
                     PETEIRLIVGAVGTCLVRNAGIVFSQDQPVRMCENADKDIVIEGGIWSDQNNEGRGRG
                     GTYDEADSMQGAHGAFLLHNVAGLAVRNARFRDCSAFAIQLGNARDFLLDGVTFDETA
                     DGIHVEGPSERGIIRSVSGKTNDDVVALNAWDWDNSSLTFGPITDVLVEDVESAPGY"
BASE COUNT          595 a          945 c          972 g          540 t
ORIGIN      
        1 ggtgatctgg agcgcttccg tgacccggct gaatctggga gaacggagtt gtgaacctgc
       61 ttcacgttcg ccgcacagtg ccactcatgc tgtgggcatc ttcgttgcgc aacgctgtcc
      121 catcggtcct atatgccgta tctccgtctt gtgcccgcgg ggtcaccgcg accgcaagac
      181 aaccccccaa tgaacgacgg ttggaaggaa aaccggtccc atgaccacgc tgcataacca
      241 atccttcttc tgggtgcgcg ggttcgggta tcaacccagt tacgcgccca ccgggctgga
      301 aatctggctc catttcgacg aacgcctgat cgccaccgag ctggcccgtg cgaatgagca
      361 tttccccggc acgaatgcca tccgactctg gctctcgttc gatgcctggt tgcgcgaacc
      421 cgcgcgtttc gtccggaact tcgggcgggt gctggacatc ctcggccatc tcggtctgcg
      481 cgcgatgccg tgcctgttca acaactggcg cagcctgccc gatttcggcg gcatcaacgc
      541 ggaaatggtc ggatactgga actcgcccca ccgggaggtc aactacttcc accgttacgc
      601 gcgcgacctc gtcacaccat tcctcaacga tgagcgaatc ctggtgtggg acctgtgcaa
      661 cgaaccgttc aacagcggga gggaggaggt gttcgtccca tggcttgcag acctgcacgg
      721 gttcgtgaaa ggaacaggcg cggtggctcc cgtcacgatc ggcgtcccac ctgacctgca
      781 ccagcttgaa ctggtggagc cgatgtgcga cgtactcacg ccccacctct acggcgccag
      841 cgcggcaaac gacgccttca tcgagttcgc gaaccgtgtc gcgaagccga tgttcgccgg
      901 cgagacgggt tggggaagcc tcgacgacga cgtacgtcta gaaacactcc aaggcgaact
      961 gtcgagcctc ctcgagcgca gggtgggctt catgattcac gttctccacc acagcctcgt
     1021 tgccgattgc caccgcgccg agttcggccc ggtgagcgag gtagggttca tgggctgcat
     1081 cgaagccgac ggcaccatgc gcgccggcta tgaatccata cggaagtatt ttgcgcaatg
     1141 aaggagctgc acccatgtca gaacttcatc acacggacgt gtttgtctcc gggcaggacg
     1201 gatttgcgtt ctaccgcatc cctgtcatcg aagtggcggc cgatggttcc atcctcgcgt
     1261 tcgtcgaggc gcggaagcat aacctcggcg atccggggca ggacaagaac cacattgcgc
     1321 ttgccatgaa acgcagcact gacggcggcc gcacgtggtc gcccatggtg atcattgaag
     1381 accccggtga ggggtggtcc gcggccaacc cggcgacggt tttcgaccgc gacaccggac
     1441 gcgtgtggct gcattatctc cgaagcaaac cggggcgagg ctccgagcat gcgcgcccgg
     1501 gcaccgacga tctccagaac ctcgtccgga cgagcgacga caacggggtg agctggtcgg
     1561 aaccgaccga catcaccacc gtctgccgcg acatggccga tccgaagtgg cgctgcacgg
     1621 tggtggggcc aggcggtggc attcaggaca gcaagggccg gctcatggtg gcctgctgga
     1681 tgaccgaacc gatgcgcgtg tttgccgtgt tcagcgatga tcacggccac acgtggcagc
     1741 gaggcggctt cgttcccggg ggaattctgg ggaacgaaga ccagttggtc gagctcgccg
     1801 acggccgcat cctcctcgat ttccggcagg cggagggcga tcatcgctgg ttcgccgaaa
     1861 gcaccgacgg cgggcggaca tggtcgcaac cgcgcgtggg agaaccggtc tctcccgtcg
     1921 cctgcgcaat cgaacgcttc actctgaaat ccgccggcga cgacaaaaac cgcatcatct
     1981 ggtcagggcc caaaggcccg ggccgcgaga atctcgttgt ccgcatcagc gaagacgaag
     2041 cggcgagttt cccgaacgaa ctcctcgtgg caacgggcaa agcagcatat gtggacgcgg
     2101 cgcggctgaa agatggcagt atcgggctcc tctgggaacg cgagcattac cagttcatca
     2161 cgttcacaac gctcgatctg gcatttctga catcgaccgg ataacgcgcg acgggaggca
     2221 acactggtga cggaaacgga acttctgggc caacttcgcg gcatcgagaa ccgcgtccct
     2281 tgtggtggcg caggcacgcc gcgggacgtg accgggtacg catcactcgc acgcagaagg
     2341 acgtggacgc agagatggga tgccgacgcg agaacgagcc gggagcctca tgaagtcacc
     2401 ggaaccgtct ggtcggatgc tatcgagcgt tgtcttgcgg aaacaggctc ggtgctgatc
     2461 ccccgcatgg aagctccggt ctaccttgac cgtcccatcg tcctcgggag cggcgcccgg
     2521 ttgatcgtcc atccggagac cgagatccgc ctcatcgtgg gagccgtcgg aacgtgcctt
     2581 gtgcgcaacg cgggaattgt cttctcgcag gatcagccgg tccggatgtg cgagaacgcg
     2641 gacaaagaca ttgtgatcga gggcggcatc tggagcgacc agaacaacga agggcgcggc
     2701 cgcggcggca cgtatgacga agcggattcc atgcagggcg cccacggagc attcctgttg
     2761 cacaacgtcg ccggattggc cgtacgcaac gcgcggttcc gggattgctc agctttcgcc
     2821 atccagcttg gaaacgcgcg ggatttcctg ctggacggcg tcaccttcga cgaaacggcc
     2881 gatggcatcc acgtcgaggg gccgtcggaa cgcgggatca tccggagtgt ttccggcaaa
     2941 accaacgacg acgtggtcgc gctcaacgcg tgggactggg ataacagcag tctcacgttt
     3001 ggcccgatta ccgatgtgct ggtggaggat gtcgaaagcg cgcccgggta ca
//
LOCUS       DAOTNG010000353         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25329_length_3035_cov_5.430537, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000353 DAOTNG010000000
VERSION     DAOTNG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25329_length_3035_cov_5.430537"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="bshC"
                     /locus_tag="P5179_13055"
     CDS             <1..1056
                     /gene="bshC"
                     /locus_tag="P5179_13055"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRS96189.1"
                     /translation="REAFAPGATLVDAFARLMVTWLGRYGIIFANPADRRFKGLLQPL
                     FSRCSSDPVGFRDAFVEQSSRVRTEGYVPQIVTSPEATLFFWDDETGNRRRVDFTPTQ
                     GFFWREGATVHTIPEFEKIASVSPERVSPNAAFRPVSGDSVFPTLVHVMGPGEIAYMA
                     QVRALYDMSGVPMPLIAARAGFTVVPQEIGSWLQSRSLAIEEALQPLDVWRHTLAERE
                     KRSSLGGRIAETRRALDETYEKLEHEVRNVLPGMDTAVLSARIKTQGLMNKLEAKLDQ
                     GFRRRVRETMPQLERISSFVFPGGVPQERVYSAAPFVARYGEAFFADLLSQIRPFDPV
                     HTCMWGESFGLDDLPVS"
     gene            1086..1826
                     /locus_tag="P5179_13060"
     CDS             1086..1826
                     /locus_tag="P5179_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96190.1"
                     /translation="MIGATIVLTAVYWLSDLAVAMWMGGLLTVSLLLGLPLDVSPAGD
                     PMLLANGASTAVEMILRPRPYIFGAALILLASVVIGVILERRARRSFAWWVIWLLRSL
                     SGLFMFIVAIYSFGNLDPYITKMQNSVDTLKKSLAVQASAESAVQDGSQELSRVMENP
                     YDAATVETLKADFTALQNRYRHWMWIQLLLGSAVLFAGALKQAAWRRSWGKEKEQAAE
                     GPDKESSAPLQSPEKDGRNPEGETPQPR"
     gene            1823..2365
                     /locus_tag="P5179_13065"
     CDS             1823..2365
                     /locus_tag="P5179_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96191.1"
                     /translation="MRFFPRTRKRVDEATPEWSVLWAVNVARVESVFYTVEAITANNV
                     CPVRLFAPGSRTELVRLPALSSGACLHPFSRIRLIEASETGFRFQASCSSQSAALAIR
                     GTRGSGVVTLALESRSGERPSAVVDGDIAPRVTLMSRHGICEIRNRILVMEAASEPLG
                     EEPRGSMWACADLVLGGSSA"
     gene            2362..>3035
                     /locus_tag="P5179_13070"
     CDS             2362..>3035
                     /locus_tag="P5179_13070"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS96192.1"
                     /translation="MNTVPAGFPAPKIGNQTAVLTHRTIRDHVYHLYRVPDLDRLVDE
                     IDPDTWKQDEKMPYWAELWPSGVALAELLASDAAALRHARVLELGCGLGLPSIVAAHL
                     GAQVTATDYYPEALDLLHVNAALNGVSLRTREVDWRTPPDLGRFDVVIAADVLYERWQ
                     TPAMVDMLARTVLPNGTAIIVDPERVTARDFAGAATFRGFRVETSYRRAENEAIPLII
                     YRLTWRT"
BASE COUNT          547 a          912 c          954 g          622 t
ORIGIN      
        1 cgggaagcct tcgccccggg ggctaccctt gtggatgcat ttgcgcgcct catggtcacg
       61 tggctgggtc gctacgggat cattttcgcc aaccccgccg acagacgatt caaaggcctt
      121 ctccagccac ttttttcgcg gtgcagttcg gatcccgtgg gatttcggga cgcattcgtc
      181 gagcaaagct cacgcgtccg gacagagggg tacgttccgc agattgtcac ctcgcctgag
      241 gcaacgctct tcttctggga tgacgaaacc ggcaaccgcc ggcgtgtgga tttcacgccg
      301 acgcagggct tcttctggcg ggaaggtgcc acagtgcaca ccatccccga attcgagaag
      361 attgcgtccg tgtcgccgga acgtgtcagt ccgaacgcag cattccgccc tgtttcaggc
      421 gattcggtgt ttccgacgct ggtgcacgtg atggggcccg gcgaaattgc gtacatggcc
      481 caggtgcgcg ctctttacga catgtctggc gttccaatgc cgctgatagc cgcgcgggca
      541 ggttttacgg tggtgccgca ggaaatcgga agctggctgc agagccgctc ccttgcaatc
      601 gaagaggcgt tgcaaccgct ggatgtgtgg cgtcacacgc tggctgagcg tgagaagcgc
      661 agcagcctcg gcggccgcat tgccgaaacc cggcgggcgc ttgacgagac atacgagaag
      721 ctcgaacatg aggttcggaa cgtactgccg gggatggata cagccgttct ctcggcccgt
      781 atcaagacgc aggggctgat gaacaaactc gaagcgaaac tggatcaggg atttcgccgg
      841 cgcgtgcggg aaaccatgcc gcagctggaa cgcataagca gttttgtgtt cccgggcggg
      901 gttccgcagg aacgtgtata ttctgctgca ccgtttgttg ctcggtacgg ggaggcgttc
      961 ttcgcggacc tgctgtcgca gattcgcccg ttcgacccgg ttcacacctg tatgtggggc
     1021 gaatcattcg gtctggacga ccttcctgtc tcgtaagggg ttcgtgccag agggagggat
     1081 atgccatgat cggcgcaacc attgtgctta ccgcggttta ctggctctcc gaccttgccg
     1141 tcgcgatgtg gatgggcggc cttctgacgg tgagcctgct cctgggtctt ccgctcgatg
     1201 tctcccccgc cggggacccc atgctcctcg ccaacggcgc ctcaaccgcg gtcgaaatga
     1261 ttcttcggcc ccgtccctac atcttcggtg ccgcgctcat cctccttgcc agtgttgtta
     1321 tcggtgtcat ccttgaacgc cgtgcgagga gaagcttcgc gtggtgggtg atctggttgc
     1381 tcagatcgct ttcagggctg ttcatgttca ttgtcgcgat ctactcattc gggaatctcg
     1441 atccctacat cacgaagatg cagaacagcg tggacaccct caagaaatcg ctggcggttc
     1501 aggcatccgc ggaaagcgct gtgcaggatg gaagccagga actctcccgc gtcatggaga
     1561 atccctacga tgccgccacc gtggaaacgc tgaaagccga tttcaccgcc cttcagaatc
     1621 gctaccggca ctggatgtgg atacagttgc tcctcggtag cgcggtcctg ttcgcgggag
     1681 cgctcaagca ggcggcgtgg cggcgttcgt ggggcaagga aaaggagcaa gccgctgaag
     1741 gccctgacaa agaatcttcc gcgccgctcc agtccccgga gaaagacggg aggaacccgg
     1801 aaggggagac gccgcaaccg cgatgaggtt ctttccgcgc acgcgaaaac gggttgacga
     1861 agcaaccccg gaatggtccg ttttgtgggc ggtgaacgtt gctcgcgtcg agtcggtttt
     1921 ctacacggtc gaagcgatca cggcgaacaa tgtgtgcccg gtgcgtttgt tcgcccccgg
     1981 aagccgcacg gaactcgtcc gtctgcctgc actttcttcc ggtgcgtgcc tccacccgtt
     2041 ctcccgcatc cggctgatcg aggcttcgga gaccggattc cggttccagg cttcctgttc
     2101 ttcgcaatcc gccgctcttg cgatcagagg cacacgcggt tcaggcgtgg tgactctggc
     2161 tctggaaagc cgatccggcg aacgcccgtc cgcagtggtg gacggcgaca ttgctccccg
     2221 cgtgacactg atgtcgcggc acgggatctg tgagattcgc aacaggattc tggtgatgga
     2281 ggcggcgtcg gaaccgcttg gggaagaacc acgcggctct atgtgggcat gcgcggacct
     2341 tgtgcttggc gggagcagcg catgaacacg gtccccgccg gtttccccgc gccgaaaatc
     2401 ggcaaccaga ctgccgttct gacccatcgc acgatccggg accacgtgta tcacctctac
     2461 cgggtgcccg acctcgacag gctggtggat gaaatagatc cggacacatg gaaacaggac
     2521 gagaaaatgc cctactgggc ggagctgtgg ccaagtggcg ttgcgctggc cgagctcctc
     2581 gcctcggatg cggccgcact ccgccatgca cgggtgctgg aacttggctg cggacttggc
     2641 ctgccaagca tcgtcgccgc acatctgggt gctcaggtga ccgccacgga ttactacccc
     2701 gaggcgctgg acctgctcca cgtaaacgcg gcgctgaacg gcgtatctct gcgcacgcgg
     2761 gaggtggact ggcgcactcc gccagatctc ggtcggttcg atgtcgtgat tgcggcggat
     2821 gtgttgtacg aacgctggca aactcctgcc atggtggaca tgctggcgag aaccgtactc
     2881 cccaatggca ccgcgattat cgttgacccc gaacgggtga ccgcacggga ttttgcaggt
     2941 gccgccacgt tccggggctt ccgcgtcgaa acctcctatc gccgggcgga aaacgaagct
     3001 attcccctta ttatttaccg acttacgtgg agaac
//
LOCUS       DAOTNG010000354         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25470_length_3022_cov_4.019211, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000354 DAOTNG010000000
VERSION     DAOTNG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25470_length_3022_cov_4.019211"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1320)
                     /locus_tag="P5179_13075"
     CDS             complement(<1..>1320)
                     /locus_tag="P5179_13075"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRS96193.1"
                     /translation="FLPTEWMAREVVGERLRWAVGEKCGFDLGINANDGIARTGRGRI
                     YVRGLITIHGALSVPLDVNLYDEVDDLNPDHFLWARQRLDASHYAREIAFACGRFPGE
                     GMDARFQSGTQHHWHVKCRRCRNADNVLELAFPDCVKRHGEGYALACTRCGGTLDAAN
                     GRWVAHYPDRQHMSFRVSALGMSALSLDRLMDEWDLALREKRLMAPFRCSKLALPDAA
                     ERQALTAQDLINACAPDAAISRPVYVGVDTGDWCHIAMAEPVDGALVYRLFDRVPGEE
                     LVARLVMLASQHRIAGILVDERPEGSLARAVCRAFPRVAMMQQFTAADYARRKTLAGE
                     TFGVLSFDREDTLGAWCDQVRMAPPRVLFPRFVEGMPFVESAPGRHILTGAQRMEVEQ
                     DGFTIHRFRGGGVDNHYFMACVFAWRMWAHSARGWREREDISLLGAIT"
     assembly_gap    1321..1420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1421..1610)
                     /locus_tag="P5179_13080"
     CDS             complement(<1421..1610)
                     /locus_tag="P5179_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96194.1"
                     /translation="MASTTRPRARRAPRPALERPTLPEFLAASIQTRDGPFTFAGRAP
                     LVGICDALGNPAIRRVDIL"
     gene            complement(1794..1988)
                     /locus_tag="P5179_13085"
     CDS             complement(1794..1988)
                     /locus_tag="P5179_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96195.1"
                     /translation="MIGFLRGKKTYFVAAGWIAWGIWTYAVERDPVAGTQRILEGVSL
                     ITLRAGVAKTGAGLEGHASS"
     gene            complement(1985..2260)
                     /locus_tag="P5179_13090"
     CDS             complement(1985..2260)
                     /locus_tag="P5179_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96196.1"
                     /translation="MTRRHRIRYQDWLMPLWAASTAALGSYIGVIRAISDLREEVRVV
                     RADYTATARALDGRLERAEAAATQTERRCESLVRHHEQAFHHRGRGE"
     gene            2488..>3022
                     /locus_tag="P5179_13095"
     CDS             2488..>3022
                     /locus_tag="P5179_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRS96197.1"
                     /translation="MKTIAALTQIPGKPKRMRVEFRNGESVELGVDIIARHRLLVGHS
                     VSAETLRAAREEDARKEAEAAALKLLNRKNCSEQELRRALKANGFGERVVDEVVEKCR
                     GWGYLNDRRLAEMVVTDAIERRHLGPARIRQTLRKRGVDDALGASAQKRAADSEPSLV
                     EQALAALKGKERTYARLD"
BASE COUNT          495 a          994 c          981 g          452 t
ORIGIN      
        1 ggtgatggcg ccgagaaggg agatgtcctc ccgctcgcgc cagccacggg cactatgcgc
       61 ccacatgcgc cacgcgaata cgcacgccat gaagtagtga ttgtccacgc cgccgccgcg
      121 gaaacgatgt atcgtgaacc cgtcctgttc cacctccatc cgctgcgcgc cggtcagaat
      181 atgccgcccc ggcgccgact ccacaaacgg catcccctcc acgaaccggg gaaacaggac
      241 gcgcgggggc gccatccgca cctgatcgca ccacgcgccg agggtgtcct cccggtcgaa
      301 cgagagcacg ccgaacgtct cgcctgccag cgttttcctc cgcgcgtagt ccgccgccgt
      361 gaactgctgc atcatggcga cgcgggggaa cgcccggcaa acggcgcgcg caaggctgcc
      421 ctcggggcgc tcgtccacaa ggatgccggc gatgcggtgc tgcgaagcca gcatcaccag
      481 ccgcgccaca agctcctccc ccggcacgcg gtcgaacagg cgatagacaa gcgcgccatc
      541 caccggctcc gccatcgcaa tgtggcacca gtcgcccgta tccacgccga catacaccgg
      601 gcgcgagatc gccgcatccg gcgcgcaggc gttgatcagg tcctgcgcgg taagcgcctg
      661 ccgttccgcc gcgtcgggaa gggcgagctt ggagcaccgg aacggcgcca tgagccgctt
      721 ttcccgcagg gcaaggtccc attcgtccat gagccggtcc agcgacagcg cggacatgcc
      781 gagtgcgctg acccggaagc tcatgtgctg ccggtcgggg tagtgcgcca cccaccgtcc
      841 gttcgcggcg tccaatgtcc cgccgcatcg ggtacaggcc agcgcgtatc cctctccgtg
      901 gcgcttcacg cagtccggga acgcaagctc cagcacatta tcggcgttcc ggcatcggcg
      961 gcatttgacg tgccagtggt gctgagtgcc gctctggaac cgcgcgtcca tcccttcgcc
     1021 gggaaagcgg ccgcaggcaa acgcgatctc ccgcgcgtag tgcgaagcgt ccagccgttg
     1081 ccgcgcccag aggaagtggt ccgggttgag atcgtccacc tcgtcgtaca ggttcacgtc
     1141 cagcggcacg gagagggcgc cgtggatggt gatgagcccc cgcacgtaga tgcgccctcg
     1201 gcccgtccgc gcgataccgt cgttggcgtt gatgccgagg tcgaacccgc acttctcccc
     1261 aaccgcccag cgcaggcgtt cgcccaccac ctcccgcgcc atccactcgg tgggcaggaa
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgaggatgtc cacacggcgg
     1441 atggctgggt tgcccagtgc gtcgcagatg cccacaagcg gggcacgccc agcgaaggtg
     1501 aacgggccgt cccgcgtctg gatggatgcg gcgagaaatt ccggcagcgt gggacgttcc
     1561 agcgcgggcc gcggtgcccg gcgtgctcgt ggacgggttg tggatgccat ggtttccttt
     1621 ctgacatgtg gtgttgatat cggaggtggt gccccggcgc ctcgatacta cgaaccggcc
     1681 agggaccgca cgcaacggag ggcacgacag agcgtgcccc tccagtcgtt tctggttcga
     1741 cacacgcccg cgcggccctt cgacaggctt cctgcgccgt gccgcggaga aggtcacgac
     1801 gaagcgtgac cctccaaccc tgcacccgtc ttcgccacgc cggcgcgcag ggtgatgagg
     1861 ctcacgccct cgaggatgcg ctgggtgccg gccaccgggt cgcgctccac ggcatacgtc
     1921 cagatgcccc atgcgatcca gcctgccgcc acgaaatagg tctttttgcc cctgagaaat
     1981 ccgatcattc gcccctcccc cggtggtgaa atgcctgttc gtgatgcctg acgagtgatt
     2041 cgcaccggcg ctccgtctgc gtggcggccg cttcggcgcg ctccagccgc ccgtcgagcg
     2101 cccgcgccgt ggcagtgtaa tccgcccgga cgacgcgcac ctcctcgcga aggtcgctga
     2161 tggcccgtat gacgccgatg tagctcccca gcgccgccgt tgatgctgcc cagaggggca
     2221 tgagccagtc ctgatacctg atcctgtgtc gccgcgtcaa cgcgaacctc cttcctgaaa
     2281 tggcttcccg tggtggtgcc ggagtgattt cccgagacgt attccccggc cgtttccagg
     2341 tgaggcaaaa cgtcgtcttc gtgtcaagac cctccgcaag ttttttttcg cggcccggat
     2401 gaggcggcag accggcgtca gcgcggggac actcacttgc cgcggggcag ggaagcaggc
     2461 attcttagag ggaaaggagc gcccgccatg aagacgattg ccgcgctgac acagataccg
     2521 ggcaagccga aacggatgcg cgtggagttc cggaacggcg aatcggtgga gctgggggtg
     2581 gatatcatcg cccggcaccg gctgctggtg gggcacagcg tgtcggcgga gacgcttcgg
     2641 gccgcccggg aagaggatgc ccggaaggag gccgaagccg ctgccctgaa gctgctgaac
     2701 cgcaaaaact gctcggagca ggagctgcgc cgggcgctga aggcgaacgg gttcggcgaa
     2761 cgggtggtgg acgaggtggt cgagaagtgc cgcggatggg gctacctcaa cgaccggcgg
     2821 ctggcggaga tggtggtgac ggacgccatc gaacggcgcc acctcggtcc cgcgcggata
     2881 cggcagacgc tccggaaacg tggtgtggac gatgcactgg gcgcctcggc gcagaaacgc
     2941 gctgcggatt cggagccatc actcgtggag caggcgctgg cggcgctgaa ggggaaggag
     3001 cgcacctacg cccggctcga cc
//
LOCUS       DAOTNG010000355         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25520_length_3017_cov_3.284605, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000355 DAOTNG010000000
VERSION     DAOTNG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25520_length_3017_cov_3.284605"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1387)
                     /locus_tag="P5179_13100"
     CDS             complement(104..1387)
                     /locus_tag="P5179_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS96198.1"
                     /translation="MPSGQKIRVVHIITTFSVGGATETALLCAAGLNATGRYEVTILA
                     GPPNSAEGSLLDDAERWGVRVDTIPALQRDISPWQDVAAFFQLTNALRKSGCAIAHTH
                     SSKAGILGRFAARVANVPLIVHTFHGLPYTTTSPVLQRVGYQMIERCAGRISSGTFSV
                     THDILRRLKEARIGNPERSWLARSGMDLDRFLRPDPGREEVRRRWGVHSDDVLLGMVG
                     RVHTGKGQDVIVRLAPEILRDAPNAKIAIIGTGPLKDRLQEQVARAGLSDRVLFVGGI
                     PVDEMPEAISALDVLVHVSEREGLARVIVQALACGKPVISYALDGSPEVIADRMNGRL
                     IPPGNDDALREAIRELVRDPRLREEWGAKGPAAVDPEFRAETMIEQIEAGYRDLLRHA
                     GIACSASSEVRPFAEWPPSSRETPVSEGSTSLGNS"
     gene            complement(1667..>3017)
                     /locus_tag="P5179_13105"
     CDS             complement(1667..>3017)
                     /locus_tag="P5179_13105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS96199.1"
                     /translation="SPIRYGGFSHGEHRRAARLRTHRGTHWGQTFDRDRGEDESQPLI
                     LYQTLSHTVTGNTRETPVSRAALPRRPSRNPRGDGEVAPVIRVLHVINTLAPGGAETL
                     VANLCAKLPGEGVEPTVAYLFGDATLAERLTENGVHVEALSSGERPSAFALCRILQII
                     RTRKIQLVHAHLVYAGIAAKIAAGLSGIPVVTTRHSAFEPKANTFFYRFDNWLTRRYA
                     ARLIAVGEQVRAAILNERWMEPERVVLHRNAIDVSEFTPKRHQPSPDGAYILGTVGRM
                     EAPKAQEVFLEAVARVRREYPNVEAVIAGDGRRRRHLEEARSRLGLDTVVTFLGPVSR
                     KDIPRVLNTFDVFVLSSNWEGLPIALIEAAASGLPVVATDVGGVHEIVQNGKNGWLVE
                     PGNVEQLAGRIGALLSDVEMRERFGREARAFVEAEFDITRLARETAALYREVLNSPA"
BASE COUNT          582 a          939 c          871 g          625 t
ORIGIN      
        1 cactccgaat gccgcttcgg ccgcgacgaa ggtgatattg agggcgattc cgattgcgaa
       61 tgcgcgcgaa taatcgtacg tgccgtggcc atgagtcccg tggttatgag tttccaagcg
      121 atgtgctccc ctctgataca ggtgtttcgc gtgatgaagg cggccactcc gcaaatggcc
      181 gcacctctga cgaagcagaa cacgcgatgc cggcatgccg caaaaggtcg cgataacccg
      241 cctctatctg ttcgatcatg gtttctgcgc ggaactccgg atcaaccgcg gcaggacctt
      301 tcgcgcccca ctcttcgcgg agtcggggat cgcgaacgag ctcacgtatg gcctcgcgca
      361 gcgcatcgtc gttgcctgga gggatcagcc ttccattcat gcggtcagca ataacttcgg
      421 gagatccgtc gagagcgtag ctgatcaccg gtttcccgca cgccagtgcc tggacaataa
      481 cccttgccag accttcgcgt tcggagacgt gcaccagtac atccaacgcc gatatagctt
      541 ccggcatttc atccacggga atgccgccca cgaacaacac cctgtcgctc aatccggccc
      601 gggcaacttg ttcctgcaac cgatccttca gcggccccgt gccgataatg gcgattttcg
      661 cgttcggagc gtctctgaga atttccggcg cgaggcgcac gataacgtcc tgcccttttc
      721 cggtgtgcac tctcccgacc atgccgagga gaacgtcatc agaatgcact ccccagcgtc
      781 ttcgaacctc ctcacgcccg ggatccggcc tcagaaaacg gtccaggtcc atcccgctcc
      841 gcgcgagcca ggaacgctcc gggtttccga ttctggcttc tttcaagcgt ctcaggatgt
      901 cgtgcgtgac cgaaaatgtt ccggacgaga ttcttcccgc acagcgctcg atcatctggt
      961 atccgacgcg ctgcagaacg ggggacgtcg ttgtatacgg caatccatgg aacgtgtgca
     1021 cgatcaacgg gacattcgcg acacgcgccg cgaagcgacc gagaatacct gccttcgagc
     1081 tgtgtgtatg ggcgatcgcg catccggatt ttcttaacgc attcgtcagc tgaaagaaag
     1141 cggcaacgtc ttgccagggt gagatgtccc tctgcaacgc cgggatcgtg tcaacccgca
     1201 ctccccaacg ttctgcgtcg tcaagaaggc ttccctcggc gctgttcggc gggcctgcaa
     1261 ggatcgtcac ctcgtaccgc ccggtagcgt tcaggccggc cgcgcaaagc aaagcagtct
     1321 cagttgcccc accgacgctg aacgtcgtga tgatgtgaac gacccggatt ttctgacctg
     1381 acggcatagt tctctctgat tgcgcgcgat ctggctcgaa gcgcgtgttc cttatgggcg
     1441 cggctcctgc accacctgac ggggttcggc caacgatctg gaaactcagg tcaaggaacg
     1501 ggacccatat gttccccgtg ggggttaacg gcagaagccc aattctttac gaaagatagg
     1561 gggtaagtcc gtgtggcggc aattcgacga aacgcccgca cggtcggaat gaccatacct
     1621 ggtgcaacgc cggtttccct gcccgccaac gcggcgggcg ggagcttcat gcgggagaat
     1681 tcaggacttc ccggtacagg gctgcagttt cgcgtgcgag tcgtgtaatg tcaaactccg
     1741 cttcgacaaa cgcgcgtgct tcgcggccga atctctcacg catctcaacg tccgatagaa
     1801 gcgcacctat tcttccggcc agctgctcaa cgttccccgg ctcaacaagc catccatttt
     1861 tcccgttttg aacgatttca tgaacgccgc ccacgtcggt ggcgacgacc ggcaatcctg
     1921 aagcggcggc ctcgataaga gcgatcggca ggccttccca gtttgacgaa agcacaaata
     1981 cgtcgaaagt gttcagcact ctagggatgt ccttccgcga taccgggccc aggaaagtga
     2041 cgacggtgtc cagtcccagt ctactgcggg cttcttccag atgacggcga cgccttccat
     2101 caccagcaat aactgcctcc acattggggt attccctccg aacgcgagcg acagcttcaa
     2161 gaaacacttc ctgcgctttc ggtgcctcca tcctgccgac ggtgccgagg atatacgccc
     2221 catccggcga tggctgatgg cgcttcggcg tgaattctga gacgtcgatt gcattccggt
     2281 ggagcaccac tcgttccggc tccatccacc tttcgttgag gatagccgca cgcacctgct
     2341 cgccaacggc gatgagtctg gccgcgtacc ggcgtgtcaa ccaattgtcg aagcgataga
     2401 agaaagtgtt ggccttcggc tcgaatgcgg agtgccgggt cgttacgacg ggtatgccgc
     2461 tcagccccgc cgcgattttg gctgcgatac ccgcgtatac aaggtgcgcg tgcacgagct
     2521 ggatttttcg ggtgcgaatg atctgaagta tgcggcaaag cgcgaaagcc gatgggcgct
     2581 ccccggaact gagcgcttcg acatgaaccc cgttttccgt caatcgttct gctagcgtgg
     2641 cgtccccaaa gaggtaggcc acggttggct ccacaccttc gcccgggagt ttcgcgcaaa
     2701 ggttcgcgac gagggtttcg gctccaccag gtgcgagggt gttgatgacg tgcaggactc
     2761 ggatcacagg tgcgacctcc ccgtcgcctc tcgggttgcg acttgggcgc cggggcaatg
     2821 cggctcgcga gacaggagtc tccctggtgt ttcccgtaac ggtatgtgac agtgtttgat
     2881 acaggatcag cggctgcgat tcgtcttcgc cgcggtctcg atcaaacgtc tgcccccagt
     2941 gcgtgccgcg gtgcgttcgg agtctcgcag cgcgtcggtg ctcaccgtgc gaaaagcccc
     3001 cgtagcgaat cgggctt
//
LOCUS       DAOTNG010000356         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25576_length_3012_cov_5.054109, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000356 DAOTNG010000000
VERSION     DAOTNG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25576_length_3012_cov_5.054109"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="P5179_13110"
     CDS             complement(<1..674)
                     /locus_tag="P5179_13110"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRS96200.1"
                     /translation="MPDFGFGSTFAALAAFWLGVLSAVSPCGLATNIAAIAFIGKNVK
                     SPSSTVWSGLFYALGRITTYVVIGMVVVAGLTAVHVLSNFLQTSMNRFLGPLLVLVGM
                     VTLDLLPLRLPETGFLTRFSSNTKVQGIAGAFGIGLVFALAFCPVSAALFFGGLIPLA
                     VEKQSTLLLPFIFGLGTAIPVAAFAVVIGAGAYSLSKLFAAVTAVELWARWVTGVVFV
                     GIGIYFT"
     gene            complement(674..1288)
                     /locus_tag="P5179_13115"
     CDS             complement(674..1288)
                     /locus_tag="P5179_13115"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRS96201.1"
                     /translation="MNAKRWVGAGLLVFVFGSLGVMGFRSLRGGAAQAESTPRLEPTS
                     SPAPMTPPVQSGEESSVGRPPGPGRPKPAVPAPEPERVQTPAQATPESNGHLVVYYFY
                     GNRRCYSCVTIQRLTQKALEQGFADELRRGKIEWRPTNVETPETQHFIADYRLSSQSV
                     ILSQVKNGQQVGWQNLQQVWYLLGNEEEFIAYVQDGIRAALRAL"
     gene            complement(1285..1704)
                     /locus_tag="P5179_13120"
     CDS             complement(1285..1704)
                     /locus_tag="P5179_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRS96202.1"
                     /translation="MADKPCCEPVAQNDCCNGATVLIYPCSGSSDVGEISDHAGRLLT
                     REGFGKMHCLAGIGGRVPGITLSAQAAGKILAIDGCPQDCASKTLSVAGIEDFSRVRV
                     TDLGMPKGKTPVTEENTRVVAKKGIEALRTSALRATA"
     gene            complement(1733..1966)
                     /locus_tag="P5179_13125"
     CDS             complement(1733..1966)
                     /locus_tag="P5179_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS96203.1"
                     /translation="MKKLQILGTGCPKCKKLTEATEQAARELGIEFEIEKVTDIDKIL
                     EFGVMMTPALAVDGVVKVVGKVPTPQEIKALIQ"
     gene            complement(1982..>3012)
                     /locus_tag="P5179_13130"
     CDS             complement(1982..>3012)
                     /locus_tag="P5179_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRS96204.1"
                     /translation="SGTILAVCSCTVLPLFAGIYKRGAGLGPAMAFLYSGPAINVLAI
                     VMTARVMGLELGVARAVGAVVFSVVIGLLMALIYRKDETARNNSQADTPEPEGGRPLW
                     QTAVYFAAMVGVLVFANWGKSDQTEAIWYAIWSVKWYLTAGFAILLAVLLNRWFSIPF
                     WQVGVASLIVLGMALAFPEEPVVTFGVGAVALSVITSLRTDESGDWFAASWGFAKQIT
                     PLLLFGVLAAGFALGRPGHEGIIPSQWVSAAVGGNGVGANFFASIAGAFMYFATLTEV
                     PIVQGLLGAGMGKGPALALLLAGPALSLPSMLVIRTVVGTGKTITYVSLVVVMATISG
                     LIYGAIFA"
BASE COUNT          671 a          938 c          832 g          571 t
ORIGIN      
        1 gtgaagtaga ttccgatccc gacgaaaacc accccggtta cccaccttgc ccacaactcc
       61 accgcggtga cagcggcgaa aagtttggaa agtgagtaag cgcccgcgcc gataaccacc
      121 gcgaacgccg ccaccggaat tgcagttccg agtccgaaga tgaacggaag cagcaacgtg
      181 gactgcttct ccacggcgag agggatcaat cctccaaaaa acaacgctgc ggaaaccgga
      241 caaaaggcga gtgcgaacac gaggccgatg ccgaacgcac cggcgattcc ctgaaccttc
      301 gtgttgctgc tgaatctcgt gagaaagcct gtctccggta atctgagggg aagaagatcg
      361 agggtgacca tcccgacgag aacaagcagg ggcccgagga aacggttcat ggaagtctgg
      421 aggaaattcg aaagaacgtg caccgcggtc aggccggcaa ccacaaccat gcctatcacc
      481 acgtacgtgg tgatacggcc aagggcatag aaaagcccgc tccagacagt gctggagggg
      541 ctcttcacat tcttcccgat gaacgcgatc gcggcgatgt tcgttgcaag tccgcacggg
      601 ctaacggcag aaagcacacc caaccagaat gccgcaagcg ccgcgaacgt gcttccgaaa
      661 ccgaagtcgg gcattacaac gccctcaagg cggcccggat gccgtcctga acgtacgcga
      721 tgaattcctc ctcgttgccc agaaggtacc agacctgttg aaggttctgc cacccgacct
      781 gttgtccgtt cttgacctgc gacagaatca ctgattgcga tgaaaggcgg taatccgcga
      841 tgaagtgctg cgtttcaggc gtctcgacgt tggtcggcct ccactcgatt tttccccgcc
      901 tcaattcgtc ggcaaagcct tgttccagag ctttctgggt gagcctctga atggtgacgc
      961 agctgtagca tcgccggttt ccgtagaagt agtagacgac aaggtggccg ttgctctccg
     1021 gtgtagcctg tgcgggcgtc tggacccgtt ctggctcagg cgcagggaca gccggtttcg
     1081 gccgcccagg tcccggcggc cggccaacac tcgattcttc gccggattgc acaggaggcg
     1141 tcattggcgc tggtgacgat gtcggctcca gacggggagt tgattcagcc tgagcagcac
     1201 caccacgcaa gctccggaac cccattaccc cgaggctccc gaatacgaac acgaggagcc
     1261 ctgctccaac ccagcgcttc gcgttcatgc cgttgctctc aatgctgatg tacgcaacgc
     1321 ctcgataccc tttttggcga caaccctcgt gttttcctca gtgaccggag ttttcccttt
     1381 cggcattccg agatcggtga cacgaacgcg ggagaagtcc tcgatgcctg cgacggaaag
     1441 cgttttggac gcgcagtcct gcgggcagcc gtcaatcgca aggatcttgc ctgcagcctg
     1501 tgcgctgagg gtgattccgg gtacacgccc gccaattccc gcaaggcaat gcattttccc
     1561 gaagccttct ctcgtgagca gtctgccggc gtgatcggaa atctcgccaa catcggaaga
     1621 tccggagcac gggtagatca gaacggtcgc gccgttgcag cagtcgttct gcgcaactgg
     1681 ctcgcaacag ggtttgtccg ccatttgtgt tcctttccac gggtctcgaa gtttactgga
     1741 tcagggcctt gatttcctgc ggggtcggca ctttgccgac taccttcacc actccgtcca
     1801 cggcaagcgc cggcgtcatc atcaccccga attccagaat cttgtcgatg tccgtcacct
     1861 tttcgatttc aaactcgatt cccagttcac gcgcggcctg ttccgtggcc tcggtgagct
     1921 ttttgcactt gggacagccc gtgcccagaa tctgcaactt cttcatgttg aaccttcctt
     1981 tttacgcgaa aatggccccg taaatgagcc cgcttattgt cgccatcacg acaaccaggc
     2041 tcacatacgt gattgtttta cctgttccta ccactgtacg tatgacaagc atgctgggaa
     2101 gcgaaagagc cggtccggca agcaacagcg ccagcgcggg accctttccc atccctgctc
     2161 cgagcaaacc ctggacaatc ggcacttcag ttaacgtagc aaaatacatg aacgcgcccg
     2221 cgatagatgc gaagaaattc gcacccacac cgttcccacc gaccgcagcg ctcacccact
     2281 gcgatgggat gatgccttcg tgacccggcc ggccaagggc gaaccccgca gccagcactc
     2341 cgaacagcag aagcggggtg atctgcttgg cgaaacccca tgatgcggcg aaccagtcgc
     2401 cggattcgtc ggttcgcagg ctggtgataa cggacaatgc gacggcaccg acaccgaatg
     2461 tcaccaccgg ttcctcaggg aaagccagcg ccattccgag gacgatgagc gaagccacac
     2521 caacttgcca gaacggaata gaaaaccagc gattgagcag aacggccaaa agaatcgcaa
     2581 accccgccgt caggtaccat ttcactgacc agatggcgta ccatatcgcc tctgtctggt
     2641 cggatttgcc ccagttcgca aacacgagga cgccgaccat ggcggcgaaa tacacggccg
     2701 tctgccagag cggcctcccg ccttcgggtt ccggggtgtc agcctgcgag ttgttgcggg
     2761 ctgtctcgtc ttttcggtaa atgagcgcca tcaaaaggcc gatgacgaca ctgaacacga
     2821 ctgcccctac tgcccgcgca acgccaagtt ccagacccat gacgcgtgcc gtcataacga
     2881 tggcaaggac attgatggcc ggccccgagt acagaaatgc catcgcaggg ccgagccccg
     2941 caccgcgctt gtagatcccc gcgaacaacg ggagaaccgt gcatgagcat actgcgagga
     3001 tcgtgccgga ga
//
LOCUS       DAOTNG010000357         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25600_length_3010_cov_4.613875, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000357 DAOTNG010000000
VERSION     DAOTNG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25600_length_3010_cov_4.613875"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="P5179_13135"
     CDS             <1..264
                     /locus_tag="P5179_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96205.1"
                     /translation="GTMQEIQRKEWPAAVETPLASSGHGGIDGGLVWDFAHAFRRGAP
                     SPIDVHTAMDYTLPGIYAALSAERNGERVRIPDTRTERLVTRS"
     gene            319..2475
                     /locus_tag="P5179_13140"
     CDS             319..2475
                     /locus_tag="P5179_13140"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HRS96206.1"
                     /translation="MQLTDVFPRIPPVKRLSVARINPQGEGIDFWIWFRSLQGLANRM
                     EPHLYLIDLNAMEKHKPLGAAETHWLDYYRTRFGIPTEDVESVDAVVERYKNLIDGYV
                     LYDNEQIIQTQNLAITRVGLECLLPVSPDQEHWMQRHGIVKRDDLRGKFADDWDAAEW
                     AIEHLWPSCYRKIYGNFCIHRPNWYALGHQLEDFIVMHRGMALDLPRCRTHRRSLYLY
                     RKMMESADPPGCQMNWHCAWEQEKEYVAEAARKGFFVLCSTGTPNLSIHGGIGDPNRS
                     YVQPLPARESCKAENGKVYVCFYNSDGDATWAMNNLHSGNWLVPERGKFKFGWGFLPL
                     MVKLMPGMLQYYQETKTEADCFWGPSSGAAYTYTHKWPTNLVDMYLTESRKLLDQTGQ
                     NGCNLVNWFLQDWWREVEDEKAVRREQEAMASGPGLVCGLGGSPFAKSYLAGPIPKVH
                     SVHIANVGRDNVGDIVRFSRECPTRPLFMFLFAQISVGIWEQIESELEEYKKHPEIEI
                     LSMDQFFLTLQDAVKRGLVGDELYEKTEALAETWLKAPGRHRLPLCERLAEELAHVAR
                     MPSDERRRHIGDAAWTDLVSIEIEGVASDREKFLTRFKGRKPPDATEDSDALLYVAFT
                     TAWVVVRAAIEAQGVYANHRDQCLNDFRRLCATICDVTPFERLFDAWDKWETGTPSVA
                     ELAPLCEGVAAAARKLTDALGASGEEEFTGWPPRTI"
     gene            2491..>3010
                     /locus_tag="P5179_13145"
     CDS             2491..>3010
                     /locus_tag="P5179_13145"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HRS96207.1"
                     /translation="MRLDDIFPTVPPVRRLLATAPDLENEGLDFWIWFRSLQGLVNRA
                     EPHLYLIKGGEVYKQGRDYWERHWLDYYTNPDTSPFKIPLQRQNSVDAIVERFRDRVN
                     GYILYDMADVPQTMNLAITLAGLNDCLPVAADQEPWMVRHGIPKRDDLRGRFSNDWQA
                     AEWAVDNLWPQCN"
BASE COUNT          684 a          835 c          916 g          575 t
ORIGIN      
        1 ggaaccatgc aagagattca gcgcaaagaa tggcccgccg cagtcgaaac gcccctggct
       61 tcgtcaggac atggcgggat agacgggggc ctcgtgtggg attttgcgca cgcatttcga
      121 agaggcgccc cgtcaccaat tgatgtccac accgccatgg actataccct ccccggaatc
      181 tatgcagcgc tttcagccga aagaaacggg gaacgagtcc gcatccccga tacgcggacg
      241 gaacggctgg tgacgcgttc ctgacaacag ccccgcaaaa tccggttccg ccaatactcc
      301 ccccaaggag gcaccgcaat gcagctgacc gatgtctttc cgaggattcc cccggtgaaa
      361 cggctctccg tggcgcgcat caatccgcag ggcgagggaa tcgatttctg gatatggttc
      421 agatcgctcc aggggctcgc aaaccgcatg gagcctcatc tctacctgat cgacctgaat
      481 gcgatggaga agcacaaacc cttgggcgca gcggaaacgc actggctgga ctattacagg
      541 acgcgcttcg ggatcccgac tgaggacgtg gaaagcgtcg atgccgtggt cgaacggtac
      601 aagaacctga tcgatggtta tgtgctctat gacaacgaac agatcatcca gacgcagaac
      661 ctggcaatta cccgcgtggg cctcgaatgc ctgttgcccg tgtctcccga tcaggaacac
      721 tggatgcaac gtcacgggat tgtgaaacgg gacgacctgc gcgggaagtt cgccgacgat
      781 tgggacgccg cagaatgggc gatcgagcac ctctggccct cgtgttaccg aaaaatctac
      841 gggaattttt gcatccaccg cccaaactgg tatgctttgg ggcaccagct cgaagatttc
      901 attgtcatgc acagaggcat ggcgctcgac cttccgcgtt gccgaactca caggaggtcg
      961 ctctatctct accggaaaat gatggaaagc gcggatccgc ccggctgcca gatgaactgg
     1021 cattgcgcgt gggaacagga gaaggaatac gttgccgaag cagcccggaa aggctttttc
     1081 gtcctctgca gcacaggcac gcccaacctc tcaatacacg gcggaatcgg agacccgaac
     1141 aggtcctacg tacagccatt gccggctcgc gaatcatgca aagcggaaaa tgggaaagtg
     1201 tacgtctgtt tctacaacag cgacggcgat gccacgtggg cgatgaacaa cctccactcg
     1261 ggaaactggc ttgttcccga gagaggcaag ttcaaattcg ggtggggttt cctgccgtta
     1321 atggtgaaat tgatgccggg aatgctccag tattatcagg agacgaaaac cgaggcagac
     1381 tgcttctggg gaccatcttc gggagcagca tacacgtaca cccacaagtg gccaacaaac
     1441 ctggttgaca tgtacctgac cgaatcgcgg aaactgcttg accagacagg ccagaacggg
     1501 tgtaatctcg tgaactggtt ccttcaggat tggtggcgcg aggtagagga cgagaaagca
     1561 gtgcgccgcg agcaggaagc gatggcatcc ggtccaggcc ttgtctgcgg acttggtggt
     1621 tcaccgttcg cgaaaagtta cctcgccgga ccgattccga aagtccactc ggtgcacata
     1681 gcgaatgttg gtcgcgacaa tgtgggcgac attgtacggt tctcccggga atgccccaca
     1741 agaccattgt tcatgtttct gtttgcgcag atcagcgtcg ggatctggga acagatcgag
     1801 tcggagcttg aggaatacaa aaaacatccg gaaatcgaga tcctgagcat ggaccagttc
     1861 ttcctcacgc tgcaggatgc ggtcaaacgg ggcctggtcg gcgacgagct gtatgagaaa
     1921 acagaagcct tggcggagac ttggctgaag gcacccggac gacatcggct tccgttgtgc
     1981 gaaaggctgg cggaggagct tgctcacgtg gcccggatgc cctcggacga acgaaggcgc
     2041 cacatcgggg acgccgcgtg gacggatctg gtatctatcg aaatcgaggg cgtcgcgagt
     2101 gaccgggaga aattcctgac tcgtttcaaa gggcgcaagc cgcctgacgc gactgaagat
     2161 tcggatgcac tcctctacgt cgccttcaca accgcgtggg tggttgttcg ggcggcgatc
     2221 gaggcacaag gcgtgtatgc gaaccatcgt gaccagtgtc tgaacgactt ccgcaggctc
     2281 tgcgcgacca tctgcgacgt gacccctttc gagcgcctgt ttgacgcatg ggacaagtgg
     2341 gagaccggga cgccctccgt tgccgaattg gctccccttt gtgagggagt cgctgcggcg
     2401 gcgcgcaagc tgacggatgc ccttggggcc tcgggtgaag aggaattcac cgggtggccg
     2461 ccacgtacga tctgaccgaa tggagaaaca atgagacttg acgatatctt cccgacggta
     2521 cctccagtaa gacggctctt ggccaccgcg cccgatctgg agaacgaagg gttggacttc
     2581 tggatctggt tccgctcgtt gcagggcctt gtgaaccggg ctgaaccgca cctctacctg
     2641 atcaagggag gcgaggttta caaacagggg cgcgattact gggagcgcca ctggctggat
     2701 tattacacca atcctgatac gtcgccgttc aagattccat tgcaacgcca gaacagcgtc
     2761 gacgcgattg tcgagcgctt cagggatcgc gtgaacggtt acatcctgta cgatatggcg
     2821 gacgttccgc agacgatgaa tcttgcgatc acgctggcag ggcttaatga ttgcctgcca
     2881 gtggcggctg accaggaacc gtggatggtg cgccacggaa ttccgaaacg ggatgacctg
     2941 cgtggccgtt tttcgaacga ctggcaggcc gccgagtggg cagtggacaa cctctggccg
     3001 cagtgcaacc
//
LOCUS       DAOTNG010000358         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25695_length_3002_cov_4.703427, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000358 DAOTNG010000000
VERSION     DAOTNG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25695_length_3002_cov_4.703427"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..827
                     /locus_tag="P5179_13150"
     CDS             210..827
                     /locus_tag="P5179_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS96208.1"
                     /translation="MTPKRILFIGNSITIHPPKEEIGWFGHWGMAASAPEKDFVHLLL
                     KRFAEHAGGVEPEARVESGVAFERGYATFDIAGEFMPLSGFRADLVVLAIGENVGALD
                     TEEKQGMFGAATERLLKVVRHGYEPKMVVRSCFWADAAKDAVLRKVSDRIGGTFVDIG
                     HLGSVEANYARSEREYAHAGVAAHPGDAGMAAIADSIWNALHPAG"
     gene            824..1228
                     /gene="mutT"
                     /locus_tag="P5179_13155"
     CDS             824..1228
                     /gene="mutT"
                     /locus_tag="P5179_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HRS96209.1"
                     /translation="MSSRPIEVVAAVIRRGGRVLVTQRNPDSHMGGLWEFPGGKVEPG
                     ETHAEALKRELREELGVRAIVGEKITLIRHAYPDRTVRLHFYRTELRDGDKPRPIGCA
                     DIRWVTMDELATLPMPPADAPLVCLLEGEPKT"
     gene            complement(1288..2481)
                     /locus_tag="P5179_13160"
     CDS             complement(1288..2481)
                     /locus_tag="P5179_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96210.1"
                     /translation="MALDLSNKQWPVHPSKNPEFDLSSSHVKNIEKYHHRSFTQEGAM
                     VAMPLAFPGITTPIPADECAIISLGMDSTRMVFGGTAGRAAHVFAAMTRGVTGIVHDL
                     GVIPGATACTAVMPAPDRTIFLAASGSFGGSLYSHPPVPWPYDCFQEWGFSRPGYTEK
                     ARVFSDEGIACAVFDELRKTIYGVSDRTGTFFAVETDHEEWEVRTFGKVDAVHRFSRV
                     LVRDNGGNIWGTACNGSLWRFSPEDERLVDLDIWIPCAAGREIHNRAEAFALDPVTGI
                     IYGSGSSDGFLFAFDPEQQAVRSLGKPGVSPTIRALTVGNDGRLFGMCGGDDDIAHLF
                     VYDPGEGTLRDIGTPVSLYGARTYGYVFASALTGKDGELFFGQAERMSHIWQYFPAIP
                     QRERA"
     gene            complement(2503..>3002)
                     /locus_tag="P5179_13165"
     CDS             complement(2503..>3002)
                     /locus_tag="P5179_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96211.1"
                     /translation="FYDAVASPEGDCIYGVTWREHMHLFRYWPEEGEFGRMEDFGPVT
                     QERDHSYPADTFSDHAGGLVFGADGMLYFVHSRWPEKSSFPTPEGAVGVVCQVNPQTM
                     EKKDFAYLKEEGRPSQYVSRAGRDRFGNLYFGHVGGTPSGMFRLPMDNPSGRSDLHEP
                     LRTWG"
BASE COUNT          621 a          926 c          901 g          554 t
ORIGIN      
        1 cggctggccg taccatggac atctttccca tgcatgtata gtagtcgcag cgattcgtgc
       61 acgggcggga cccctgctgc gcagggcgca gcccgtgcta cacgggtttg ttcacaaatg
      121 gagattttcg ccgcgcgtcc gcgctgcgga cgacgcacaa aatgatcaca gacggcgacg
      181 gtaatcgtcc acacgcgacg gagccacaca tgacaccaaa acgcatcctt ttcatcggca
      241 acagcatcac catccaccct cccaaagagg agatcggatg gttcgggcac tggggcatgg
      301 cggccagcgc gccggagaag gacttcgtcc atctccttct aaaacggttc gcggaacatg
      361 cgggaggagt agagccggag gcgcgtgtgg agagcggagt tgcttttgaa cgcgggtacg
      421 caacgttcga tatcgcgggg gaattcatgc cgctgtccgg attccgggcc gaccttgtag
      481 ttctggcgat cggggaaaac gtgggggccc ttgataccga agagaagcag ggtatgttcg
      541 gtgcggccac ggagcggttg ctgaaagtgg tcaggcacgg gtacgagcca aagatggtcg
      601 tgcggagctg tttctgggcc gatgcggcga aagacgcggt gttgcgcaaa gtctccgaca
      661 ggatcggggg aacctttgtg gatatcggtc accttggaag cgttgaggcg aattacgctc
      721 ggtcggagcg cgagtacgct cacgccgggg ttgcggccca cccgggcgat gcagggatgg
      781 ccgccatcgc cgattccatc tggaacgcgt tgcatcccgc cggatgagta gccgcccgat
      841 tgaagtcgtc gccgcggtaa ttcggcgggg tggcagggtt ctcgtcacgc agcgcaaccc
      901 tgattcccac atgggtggcc tctgggagtt tcccggcgga aaggtggaac cgggcgagac
      961 tcacgctgag gcgttaaaga gggaactgcg ggaggaattg ggcgtccgcg cgatcgtcgg
     1021 ggagaaaatc accctgatcc gccacgcgta ccccgatcga accgtccgtc tgcacttcta
     1081 ccgcacagaa ctgcgggacg gagacaaacc ccgccctatc gggtgcgcag atatccggtg
     1141 ggtgacgatg gacgaattgg ctacccttcc gatgccgccc gctgatgcac ctctggtctg
     1201 tctgttggag ggcgaaccaa aaacgtaggg ccagttcacg aactgccccc aaaacctcac
     1261 cggaaccccc acaccgccgc ggcgacccta cgctctttcc ctctgcggaa ttgcgggaaa
     1321 gtactgccag atgtggctca tgcgttcggc ctgcccgaaa aacaactcgc cgtccttgcc
     1381 ggtcagcgcg gacgcgaaga cgtagccgta cgttcgcgcg ccgtaaagag aaaccggggt
     1441 accgatatcg cgcaaggttc cttcaccagg atcgtacacg aacagatgag caatgtcatc
     1501 gtcgccgccg cacataccga agagccttcc atcattcccc acggtgaggg cgcggattgt
     1561 cggcgatacc cccggcttcc cgagagaacg aacagcctgt tgctccggat cgaaggcgaa
     1621 caggaacccg tctgaactcc cgctgccgta aatgattccg gttaccggat ccagcgcaaa
     1681 cgcctccgcg cggttatgaa tctcccttcc ggccgcgcag ggaatccaga tatcgagatc
     1741 cacaagtctt tcgtcttccg gggaaaaacg ccagagcgag ccattgcagg cggtgcccca
     1801 gatattgccc ccgttatcgc gcaccagcac ccgggaaaac cgatgcaccg catccacttt
     1861 gccgaaggtg cgcacttccc attcctcgtg gtctgtttcc acggcgaaaa aggtaccagt
     1921 gcggtcggag acgccgtaaa tcgtcttgcg gagttcatcg aagactgcgc aggcgatccc
     1981 ctcgtccgaa aacacgcgtg ctttttccgt gtatccggga cgcgagaaac cccattcctg
     2041 gaaacaatcg tacggccacg gcactggcgg atggctgtag agactgcctc cgaacgaacc
     2101 gctggcagcg agaaaaatgg ttcggtcggg tgccggcatg acagccgtgc aggccgtcgc
     2161 gccaggtatt actcccagat cgtgaacgat gcctgtgacg ccacgggtca tggccgcgaa
     2221 cacatgggcc gcccgcccgg ctgttccacc gaaaaccatc cgcgtggaat ccattccgag
     2281 gctgatgatg gcgcattcat ccgccggaat cggcgtggta atcccgggga atgccagcgg
     2341 catcgccacc atcgctcctt cctgcgtgaa actgcgatgg tggtatttct caatgttctt
     2401 cacgtggctc gagctgaggt cgaattccgg gtttttcgac ggatgcacgg gccactgctt
     2461 gttgctgaga tcaagtgcca tcggtgtcgc tccttggaat tgtcagcccc acgtcctcaa
     2521 cggctcgtgc aaatcgcttc gccccgacgg attgtccatc ggcagccgga acatacccga
     2581 aggcgtgccg cccacatggc cgaaatacag attgccgaac ctgtctctgc ccgcgcggga
     2641 aacgtactgg gacgggcgcc cctcttcctt cagatacgca aagtcctttt tctccatcgt
     2701 ctgcgggttc acctgacaga cgacgcctac cgcgccctcc ggcgtgggaa aggacgattt
     2761 ttccggccag cgggaatgca cgaagtagag catgccgtcc gcgccaaaca cgagcccgcc
     2821 ggcatggtcg gagaaggtat ccgcagggta agagtgatcg cgttcctgcg ttaccggccc
     2881 gaagtcctcc atgcggccga attccccctc ttccggccag taacggaaca aatgcatatg
     2941 ctcccgccac gtcaccccgt agatgcagtc cccttccggc gaagcaacgg catcataaaa
     3001 ca
//
LOCUS       DAOTNG010000359         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25836_length_2989_cov_4.801636, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000359 DAOTNG010000000
VERSION     DAOTNG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25836_length_2989_cov_4.801636"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="P5179_13170"
     CDS             complement(<1..424)
                     /locus_tag="P5179_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRS96212.1"
                     /translation="MRIIIEKDYAGVSRRAADFVEALLRRKPNAVLGLATGSTPLGLY
                     RELIRRHKDEGLDFSGVTTFNLDEYYGLAPDHPQSYHHFMRVNLFDHINVPGDNINIP
                     SGTAKNIAKSCDDYERKIREFGGIDLQILGIGSDGHIAF"
     gene            complement(482..1918)
                     /locus_tag="P5179_13175"
     CDS             complement(482..1918)
                     /locus_tag="P5179_13175"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRS96213.1"
                     /translation="MNEKPSAEVAAGVIGSRLVQALEEFLDSTSRRDEHLVKLLTVMA
                     KLAASAQTPLPGGAAHLEAADVSGVVAALEKIAERSVPAGSTAVQAPVPDITAVLEKQ
                     NQTNELLVRLITEQARRVSEAEMKQIPELSDQERWADLQIGSFDDKDIAFMKESEVFG
                     ALSDETLKVIRANATLEKYEPGKMIFVIGDPVSQVYIIKSGIVEICRPTDDPDKLNVV
                     AYLTAGDSIGEMSILISGDSRSSIARVPQGAEVLMIPHDVFMKLFKALPELALRLATV
                     FARRLKTSIKKERIQTHHRELQGSLEYFDLATIIQTLLSSDERTGVLTVTDEQQEPLA
                     DLYFEAGTMRYARYRQLLGEEAFYQLFQTENKKGSFSFKEGKFPEGFDQRAEVSVPGM
                     SLLFEAARLSDELKLLKEQIPDPDKVFKPKVDALEWTDDDNRTLANGIWNMLRRGASV
                     TELTENLPRSEYAIYAVLSEMLKSGQIE"
     gene            2172..2795
                     /locus_tag="P5179_13180"
     CDS             2172..2795
                     /locus_tag="P5179_13180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS96214.1"
                     /translation="MPDDPPVPENPRAAELKALLAEIARNEPGFFRSLVADVFKIPFG
                     YRWEDHPGVTIGKDVRIHPAARIELGSRSKLTIGDGSSIGPMAVLRPKDFHMIIGPNC
                     SVNDYTMLYDNLVLGRGVRIGAHSLIIPENHTFATRAKYIYEQPCEFKGVVFEDDVWV
                     GSNVVVLDGVRVGKGAILAAGCVVTRDVPPYTIVGGVPARVIKERPL"
     gene            complement(2807..>2989)
                     /locus_tag="P5179_13185"
     CDS             complement(2807..>2989)
                     /locus_tag="P5179_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96215.1"
                     /translation="NTDILINGPVDRIEVEARKLIELARDGGLIIGTHSVSPEVPLEH
                     FRAYDRICRRYGVFTE"
BASE COUNT          607 a          854 c          836 g          692 t
ORIGIN      
        1 tgaaggcgat gtggccatca cttccaatgc cgagtatctg cagatcaata ccaccaaatt
       61 cccggatttt tcgctcgtaa tcatcgcatg attttgcgat gttcttcgcg gttccggaag
      121 gtatgttgat gttatcgccg ggcacgttaa tgtggtcgaa caggtttacg cgcatgaaat
      181 ggtggtaact ctggggatga tcgggtgcga gcccgtaata ctcgtcgaga ttgaacgtcg
      241 tcacaccgga aaaatccaga ccctcgtctt tgtgccgtcg gatgagttcc cggtacagac
      301 ccagaggagt tgagccggtc gccagtccga ggaccgcgtt cggcttgcgc cgcagcagtg
      361 cctcaacgaa atcggccgca cggcgcgaga cgcctgcata gtccttttct atgatgattc
      421 gcacgcgcca accttctttt ctctgagagc atgcggctgc cgccgctgtg taatgctcgc
      481 cctattcaat ctgaccggat ttcagcattt cggagagcac tgcgtagatc gcgtattccg
      541 accgcggaag attctccgtc aattccgtca ccgacgcgcc tctgcgcagc atgttccaga
      601 taccgttcgc caaggtccgg ttgtcgtcat cggtccattc cagggcgtca accttcggtt
      661 tgaagacttt gtcagggtcg ggtatctgtt ccttgagaag cttgagctca tccgaaagtc
      721 gcgccgcttc gaacaacagg gacatgcctg gcaccgatac ttccgctcgc tggtcgaaac
      781 cttccgggaa tttgccttct ttgaacgaga agctcccttt tttgttctcc gtctggaaaa
      841 gctgatagaa ggcttcttcg ccaaggagct ggcggtaacg ggcataccgc atggtccccg
      901 cctcgaaata gaggtcggcc aaaggttctt gctgctcgtc ggtaaccgta aggacgcccg
      961 tcctttcgtc ggaggacaac agcgtctgga tgatggtcgc cagatcgaaa tattccagcg
     1021 atccctgaag ttcgcggtga tgggtttgaa tgcgctcctt cttgattgac gttttcagcc
     1081 ggcgcgcaaa cactgtcgcg aggcggagag cgagctctgg caacgccttg aaaagcttca
     1141 taaacacgtc atgcggaatc atcaagactt ccgcgccttg cggcacccgc gcgattgagc
     1201 tgcgggaatc cccggagatg agaatggaca tctccccgat ggaatcaccc gcggttagat
     1261 acgccacgac attcagtttg tccgggtcgt cggtcgggcg gcagatttcc acgattcctg
     1321 acttgatgat gtagacctgg gagaccggat ccccgattac gaaaatcatc ttgcccggtt
     1381 catatttctc cagcgttgcg ttcgcacgaa tgaccttcaa cgtttcgtct gacagagcac
     1441 cgaaaacttc tgattccttc atgaacgcga tatctttgtc atcgaaggaa ccgatttgaa
     1501 ggtcggccca ccgttcctga tcgctcaact cgggtatctg tttcatctcg gcttcggaca
     1561 cgcgccgcgc ctgttcagtg atcaaccgga ccaaaagttc attcgtctgg ttctgtttct
     1621 ccaggacggc ggtaatgtcg ggaacgggag cttgcaccgc ggtcgagccc gcagggacgg
     1681 atcgttctgc gatcttctcg agtgcggcga cgactccaga gacatcggcg gcttccagat
     1741 gcgccgcgcc cccgggcaaa ggggtctgcg ccgatgccgc gagtttggcc atgacggtaa
     1801 gcagtttcac gaggtgttca tcacgcctcg atgtggagtc cagaaactcc tccaacgcct
     1861 gaacaaggcg cgaaccgatc actccggcgg caacttcggc actcggtttt tcgttcattg
     1921 agaacctccc ctgagccgtt ggcatcgtgc tccggctggc gcatccacac gcgaaccgcc
     1981 cggcaccagc tgcggacggg tcactgacgt ggctccgtat attgcctaat gtacgcaaat
     2041 agcgattgag tggcgagtca aggaggggaa taagtcagca cggggggttg cgcttcgatg
     2101 gcgttggctc gttcctgtaa tttccggtat gcgaaccctc gtctcagact ttctcacgcg
     2161 gagttgaacc gatgcccgat gatccgcctg tgcctgagaa ccctcgcgcc gccgaattga
     2221 aagcgttgct ggctgagatt gcccgcaatg agcccggttt tttccggtcc ctcgtggcgg
     2281 acgtgttcaa aatcccgttc ggataccgct gggaggacca tcccggcgtt actatcggga
     2341 aagacgtacg gattcatccc gccgcacgca ttgaactggg cagccgaagc aagctcacga
     2401 taggcgacgg aagttccatt ggtcccatgg ctgttcttcg accgaaggat ttccacatga
     2461 ttatcggacc caactgctcg gtcaacgatt acaccatgct gtacgataac ctggttctcg
     2521 ggcgcggcgt gcgtatcggc gctcactcgc tcataatccc ggaaaaccac acattcgcca
     2581 cccgcgcgaa atacatctac gaacaacctt gcgagttcaa aggggtggtt tttgaagacg
     2641 acgtctgggt ggggtcgaat gttgtggttc tggacggcgt gagggttggg aagggcgcga
     2701 ttcttgccgc cggttgtgta gtcaccaggg acgtgccgcc ttacacgatt gtgggcggcg
     2761 tgccggcgcg ggtgatcaaa gaacgccctt tgtaatagat gcaccatcac tccgtgaata
     2821 ctccgtaacg ccggcagata cggtcgtagg cacggaaatg ctccagagga acctccgggc
     2881 tcacagaatg agttccgatg atcagtccac cgtcccgcgc aagctcgatc agttttcgcg
     2941 cttccacctc aattcgatcc accggcccgt ttatcaggat atccgtatt
//
LOCUS       DAOTNG010000360         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_25840_length_2989_cov_3.190866, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000360 DAOTNG010000000
VERSION     DAOTNG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25840_length_2989_cov_3.190866"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="P5179_13190"
     CDS             <1..1086
                     /locus_tag="P5179_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS96216.1"
                     /translation="GFGVRNSHGIMFEVEGMKLLLEAMAEETGVGLLYHTFFCDTVVD
                     DGAAIGAVIQNKDGRQVIRARRIVDCTGDGDVAFRAGCPYEVGRPSDGKCQPVTLMFT
                     IGGVDWPTVEKFRVDYQLKEVWERAQREGNMRPFQKTIMGWWWTPTRPDQVGVNFTHV
                     THIDSTKAADLTRATIEARKQAYETIEVYRKYVPGMEHCYMISTPNTVGIRESRRIMG
                     DYVLTEDDVKGQREFSDAICYGSFFVDIHCIDGPGMDSTSWRPSRGFKYQIPYRILVP
                     RGAENLLTAGRCVSCTHLALGSIRVMVPCIAMGEAAGTASALSLRDGVAPRAVDVTAL
                     QAQLRQQGAILYEEDIVTVERNSQMAR"
     gene            1120..1599
                     /locus_tag="P5179_13195"
     CDS             1120..1599
                     /locus_tag="P5179_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96217.1"
                     /translation="MVDTNTPLRRLISKYRDRDRATPDVGFPIHRILSTYAYERALWF
                     LAVFRMVVGFGAFSIGVMGVSELQGAEASLACVIAICGAFVSIGYRTRWAAAVLLLIW
                     LAYSFFDRELWPFGIIWLAVGIVLLAGAPSFSVDARRAARREGRTVPDPSDISGTLE"
     gene            1596..2489
                     /locus_tag="P5179_13200"
     CDS             1596..2489
                     /locus_tag="P5179_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96218.1"
                     /translation="MSLPPRSEKSPRRWDLVTPQAAFSIRDTAEPFVFGDAMWLSNGY
                     YYGGVLSRDLWRSTDGAEWTMVSDATPYDGYSEMVVFEGRVWAIKGSVWNSPDGVTWN
                     QVLDKTPFGVRGYGEVVVLNGCMWQLGSGNDVWCSRDGEKWECVCADAPFGPRYGSAV
                     AVHDGALWLMGGATQEKGDPPEKHYPDFVTHNDVWRSVDGADWECVTAHAPWSERQWV
                     VAEVYAGRLWIIGGFSNRESCNLGDVWYTENGTTWHEYEPKPGFSPRHEATPYVFDGS
                     LWVVAGNSWPLMNDVWRLTLG"
     gene            2522..>2989
                     /locus_tag="P5179_13205"
     CDS             2522..>2989
                     /locus_tag="P5179_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96219.1"
                     /translation="MKHVIVYEHPDAFVGWPANNGLWSWDGKEVLVGFTVGGFEEKPG
                     HNITEPYRSMLARSTNQGDTWKAFQPANFVRPGITATALTEPLDFTAPGFAMRVVGIG
                     YHGSDEPRGMFFASNDRGATWQGPYSFGDLMQHEELAGLSFTSEDLQDLQQRFQ"
BASE COUNT          566 a          817 c         1029 g          577 t
ORIGIN      
        1 ggcttcggcg tgcgtaacag ccacggaatc atgttcgagg tggaggggat gaagctgctg
       61 ctggaggcca tggccgagga aacgggcgtc ggcctgctct accacacctt cttctgcgat
      121 acggtcgtag acgacggcgc tgcgatcggt gccgttatcc agaacaagga cgggcggcag
      181 gttatccgcg ctcggcggat tgtggactgc acgggcgacg gcgatgtcgc gttccgggcg
      241 gggtgcccgt acgaagtggg caggccgtca gacgggaaat gccagccggt gacccttatg
      301 ttcactatcg gtggcgtgga ttggccgacc gtggagaaat tccgcgtgga ttaccagttg
      361 aaggaggtgt gggagcgcgc ccagcgcgaa ggcaatatgc ggccgttcca gaaaaccatc
      421 atggggtggt ggtggacgcc gacccggccc gatcaggtgg gggtcaattt cacccatgtg
      481 acgcacattg actcgacgaa agccgccgac ctgaccaggg cgaccattga agcgcgaaaa
      541 caggcgtacg agacgatcga agtctaccgg aagtacgtcc ccggtatgga acactgctac
      601 atgatctcaa cgccgaacac tgtcggaatt cgggaatcgc gccgcatcat gggggattac
      661 gtgctgaccg aggacgacgt gaagggtcag cgcgagttct ccgacgcaat ctgttacggc
      721 tcgttcttcg tggatattca ctgcattgat ggccccggca tggactccac aagctggcgt
      781 ccctcacgtg gcttcaaata ccagattccc taccgcatcc tcgtgccgcg cggtgcggag
      841 aacctgctga ctgccggacg gtgcgtgagc tgcacccacc tcgcgctggg ttcgatccgc
      901 gtcatggtgc cgtgcattgc catgggagag gcggccggca cggcaagcgc gctttcgctg
      961 cgggatggcg ttgcgccgcg ggccgttgat gtgacggcgt tgcaggcgca actgcggcag
     1021 cagggggcga ttctttacga ggaagacatc gtgacggtgg agaggaactc gcaaatggcg
     1081 cgttaactct gcaccgcaaa ccagaagcgg gataggttca tggtggatac caataccccg
     1141 ttgcgccggc tgatctcgaa ataccgcgac cgggatcggg caacgccgga tgtcggattt
     1201 ccaatacacc ggatactatc gacatacgct tatgagcgcg cgctttggtt tcttgcggtt
     1261 ttccgaatgg ttgtgggttt tggggcgttc tcgatcggtg ttatgggcgt ttctgaatta
     1321 cagggcgcag aagcgtcgct cgcgtgcgtg atcgcgatct gcggagcgtt tgtctcgata
     1381 ggctatcgaa cgcgatgggc tgcggcggtt ctgctcctca tctggttggc gtattctttc
     1441 tttgaccgcg aactctggcc gtttggaata atatggcttg cagtgggcat tgtgcttctc
     1501 gccggtgcgc caagtttttc cgtagatgcc agacgggcgg cgagacggga gggaagaacg
     1561 gtgcccgacc cgtcggatat ttcggggaca ctggaatgag cctgcctcca cgatcggaga
     1621 aatccccccg ccggtgggac ctcgtaaccc cgcaggcggc gttcagcatc cgcgatacgg
     1681 ccgagccgtt cgtgtttggc gatgccatgt ggctcagcaa cggctactac tacggcggcg
     1741 tgctctcgcg ggacctgtgg cgctccaccg acggggccga atggacaatg gtatccgatg
     1801 ccacgccgta cgacggctac agcgagatgg tcgtgtttga gggccgcgta tgggccatca
     1861 agggcagcgt gtggaactcc cctgacgggg tgacgtggaa tcaggtgctg gacaagacac
     1921 cgttcggagt gcggggctac ggcgaggtcg tcgtgctgaa cggctgcatg tggcaactgg
     1981 gcagcggcaa cgatgtctgg tgctcgcggg atggcgagaa atgggagtgc gtctgtgcgg
     2041 atgcgccgtt tggcccgcgg tatggctcgg cggtggccgt gcatgacggc gcgctctggc
     2101 tcatgggtgg ggccacgcag gagaagggtg atccgccgga aaagcactat cccgatttcg
     2161 tgacgcacaa cgatgtgtgg cgctcggttg acggggcaga ctgggaatgc gtgacggcac
     2221 acgcgccatg gagcgaacgc cagtgggtgg ttgccgaggt gtatgcgggc aggctctgga
     2281 tcattggcgg attcagcaac cgcgaaagct gcaaccttgg cgacgtgtgg tatactgaaa
     2341 acgggaccac gtggcacgag tacgaaccga agcccggttt ctcgccgcgc cacgaggcga
     2401 cgccgtacgt gttcgacggg agcctctggg tggtggcggg caactcgtgg ccgttgatga
     2461 atgatgtctg gaggctgacg ctgggatgaa cttcctgaaa cgcttcaagg gggaaaatgg
     2521 tatgaaacac gtcatcgtat acgagcaccc ggatgccttc gtgggatggc cggcgaacaa
     2581 cggactctgg tcgtgggatg gcaaagaggt gcttgtcggc ttcacggtgg gcggtttcga
     2641 agagaagccc gggcacaaca tcaccgagcc ctaccgctcg atgctggcgc gcagcaccaa
     2701 tcagggcgat acatggaagg cgttccagcc ggcgaacttc gtgagaccgg gtatcacggc
     2761 gactgcgctc actgagcccc tcgatttcac ggcgcccggg ttcgccatgc gggtcgtggg
     2821 gatcggctac cacggcagcg atgaaccgag agggatgttc ttcgcctcga acgaccgggg
     2881 cgccacgtgg caggggccgt actcattcgg cgacctcatg cagcatgagg aactggccgg
     2941 cctcagcttc acctccgagg atctccagga ccttcaacaa cgcttccaa
//
LOCUS       DAOTNG010000361         2965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26080_length_2965_cov_4.108591, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000361 DAOTNG010000000
VERSION     DAOTNG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26080_length_2965_cov_4.108591"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="P5179_13210"
     CDS             <1..1177
                     /locus_tag="P5179_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRS96220.1"
                     /translation="LTILGASGTIPFACTHAADTISSVVTLTPQRAFEPYETVRVIGL
                     PTLRDSTGRPTFAGDTVSFRATGPQVVATSPVNGATTRLAGNTLTVSFNTRMAMDNPQ
                     AVLVVGEQSGAHPIASLSSPDGGYTAHVALAGNFIGGEVVTATVTSAFVSAVSSFPLA
                     RPHVWRFSIQPSAPASLTKRDPVAGDLFAGGSVAAGRFTAVGDGRGVLLSDPTGSLAL
                     FSGGSGTWTQAASLTGIPGRSCTRAGDLNGDGLLDIVVAAVDSDFVQVYFNASGAAST
                     PAFEQPVRLPVGGQPVGVFIADLNGDGRPDIATANRATNDVSILLNNGNKTFAEEVFV
                     AAGNNPQAINGADLDGDGDIDLVVANATDNTITLIRNLTGFREPPQTGAKRGNGGVR"
     gene            1174..1887
                     /gene="ispD"
                     /locus_tag="P5179_13215"
     CDS             1174..1887
                     /gene="ispD"
                     /locus_tag="P5179_13215"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRS96221.1"
                     /translation="MIGEGLPVVAVVVAGGAGVRMGGGTPKQYRLLAGAPVLAWTLRA
                     FEKATDVDEVILVVPPSDLAFVGEAIVDAYRLTKIRRIVEGGATRQESTAAGVAAVEA
                     SDGVLLVHDGVRPFVSGEHIAAVVEAAWSTGAAILAVPVTDTVKRVDDRGCIEGTISR
                     ENLWAAQTPQGFRLSLFREAMARARREGFAATDEAMMVERMNHPVMIVPGDAENIKLT
                     VPEDFARAAQIAERRSRPC"
     gene            1881..2360
                     /gene="ispF"
                     /locus_tag="P5179_13220"
     CDS             1881..2360
                     /gene="ispF"
                     /locus_tag="P5179_13220"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRS96222.1"
                     /translation="MLRIGQGYDIHPLAAGRRLVLGGVEIPSDVGLEGHSDADVLVHA
                     IMDALLGALALGDIGQHFPNTDERYRGVDSIRLLERVAELIRQHGARVVNVDSTVVAE
                     RPRVAPYVPAIRERISAALGIPASAISIKATTAEKLGALGRGEGISASAIALVGDGV"
     gene            complement(2372..>2965)
                     /locus_tag="P5179_13225"
     CDS             complement(2372..>2965)
                     /locus_tag="P5179_13225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS96223.1"
                     /translation="NALPSSYSPPEKPVRSIAEHLRLVWVGSLAPIKGLSLALEALAS
                     SNTPNKLTIVGDGPLARELPGWLKNLGLEHRVDWWGQVPWLEVKRAYMENDVLLFTSL
                     RDTSGAQFLEAMAHGMPVIALDHHGAHDWIPDNAGIKVPVTNPKETVAGLAAAIDRLW
                     REPELREQMGNAAYEYAKKQTWENRVREIAGLYEQYM"
BASE COUNT          536 a          873 c          994 g          562 t
ORIGIN      
        1 gttaacaatt ctcggtgctt cgggaaccat tcccttcgcc tgtacccatg cggcagacac
       61 catttccagc gtcgtgacgc tcaccccgca acgggcattt gaaccgtatg agacggtccg
      121 cgtgatcgga ctcccgaccc tgcgtgattc gacggggagg ccaacgtttg cgggcgatac
      181 cgtctctttc cgggcaaccg gtccgcaggt ggtggcaacg agcccggtca acggtgccac
      241 cacacggctt gccggaaaca ccctcaccgt ttcgttcaac acgcgcatgg cgatggataa
      301 tccgcaggcg gttctggttg tgggggaaca gagcggcgcg catccgattg cctccctttc
      361 aagcccggat ggcggttaca cggcgcatgt cgcgctggcc ggcaatttca tcgggggcga
      421 agtcgtcacg gcgacggtaa ccagcgcgtt tgtgtccgcg gtgtcgtcgt ttcctctcgc
      481 gcgtccgcac gtgtggcgat tttcgatcca gccgtcggca ccggccagcc tgacgaaacg
      541 ggatccggtg gccggggact tgttcgccgg tggatcggtg gcagcggggc gatttaccgc
      601 agtgggtgac gggaggggag tgctgttgag cgacccgacg ggttccctcg cgttgttcag
      661 cgggggatcg gggacctgga cgcaggcggc ttccctaacc ggcattcccg gacgcagctg
      721 cacgcgggcg ggcgacctga acggcgacgg cctcctggac atcgttgtag cggctgtgga
      781 ttccgacttc gtgcaggtgt atttcaacgc ctccggagcc gcatctacgc ctgcatttga
      841 gcagccggtg cgccttcccg tcggcgggca acccgtcggg gtgtttatcg cggacctgaa
      901 tggggacggg cggccggaca tcgcaacagc aaaccgcgcc acgaacgatg tctccattct
      961 cctcaacaac ggcaacaaaa cgtttgccga ggaagtcttc gtcgcggcag gcaacaaccc
     1021 gcaggcaatc aacggcgccg atctggacgg cgatggcgac attgacctcg tggtggcgaa
     1081 cgcgaccgat aacacgatca ccttgatccg gaatcttacc ggtttccggg aacctcctca
     1141 gaccggggcg aaacgtggga acggaggggt gcgatgatcg gggaaggcct tccagttgtt
     1201 gcggttgtgg tggcgggtgg ggccggggtg cgcatgggcg gcggcacacc gaaacagtac
     1261 cgcttgctcg ccggtgcacc ggttctcgca tggacgttgc gtgccttcga gaaggcgacg
     1321 gacgtggacg aggtcatact ggtggtacca cccagcgacc ttgcattcgt aggtgaagcg
     1381 attgtcgatg cgtaccggtt gaccaaaatc cggcgcatcg tggagggagg agccacccgg
     1441 caggaatcca ctgcggcggg cgtcgcggcg gtcgaagctt ccgatggtgt tctccttgtc
     1501 catgacggtg ttcggccatt cgtgtccgga gaacacatcg cggcggttgt ggaggccgcg
     1561 tggagtactg gtgccgcgat cctcgccgtg ccggtaaccg acaccgtgaa gcgtgttgac
     1621 gacagggggt gcatcgaggg aaccatctcg cgcgagaacc tgtgggcggc acagacgccg
     1681 cagggattcc gcctgtcgtt gttcagagaa gcgatggccc gcgcccgtcg ggaagggttc
     1741 gcggcgacgg acgaagcgat gatggtggag cgaatgaacc atccggtgat gattgttccc
     1801 ggcgacgcgg agaacatcaa attgacggtg ccggaggatt tcgcgcgtgc tgctcagatt
     1861 gcggagagga ggagcaggcc gtgctgagaa tcggacaggg gtacgatatc cacccgctgg
     1921 ctgcgggcag gaggctcgtt ctcggcgggg tggaaatccc ttcggacgtg ggattggaag
     1981 gccattcgga cgcggacgtg ctggtgcacg cgatcatgga tgccctcctg ggtgcgctgg
     2041 cgctggggga tatcggccag catttcccga acacggacga gcggtaccgg ggcgtcgaca
     2101 gcatccggtt gctggaaagg gtggctgagc tgatccggca gcacggggca agggtggtca
     2161 acgtggattc aacggtggtt gccgaacgcc cgcgtgtcgc tccctatgtt cccgctatac
     2221 gggaacggat cagcgcggct cttggcattc ccgcgagcgc aatctcgatc aaagccacca
     2281 ctgcggaaaa gctgggcgca ctcggacggg gggaggggat ttcagcgagc gccattgcac
     2341 tcgttgggga cggtgtgtag ccgacaacgc gtcacatata ctgctcgtaa agacccgcaa
     2401 tctcccgcac tctgttttcc cacgtctgtt tcttcgcgta ctcgtacgcg gcgttgccca
     2461 tctgctccct caactccggt tcccgccaga gcctgtcaat tgccgcggcc agcccggcaa
     2521 ccgtttcctt cgggttggta accggaacct tgattccggc attgtccgga atccagtcgt
     2581 gtgccccgtg gtggtcgagc gcaatgacag gcataccatg ggccatggct tccaggaact
     2641 gggcgccgga cgtgtcacga aggctcgtga acaacagcac gtcgttttcc atgtacgcgc
     2701 gcttcacctc aagccagggg acctgccccc accagtccac ccggtgctca agccccagat
     2761 ttttcaacca ccccgggagt tcccgcgcaa ggggcccatc gcccacgatg gtgagtttgt
     2821 tcggggtgtt tgaggatgcc agcgcctcaa gagccaaaga aagaccttta attggtgcca
     2881 ggctccctac ccagacgagt cgcaggtgct cagcaattga acggacaggc ttttcagggg
     2941 gagaatagga actcggaagc gcgtt
//
LOCUS       DAOTNG010000362         2955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26192_length_2955_cov_4.155172, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000362 DAOTNG010000000
VERSION     DAOTNG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26192_length_2955_cov_4.155172"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="P5179_13230"
     CDS             complement(<1..1414)
                     /locus_tag="P5179_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96224.1"
                     /translation="MPHFPAERTILVRQIGNRLNELFAALYRNRTPITGWQTYVAGPG
                     QYPAPIPVDGWIPYELGDDWGGYDVTQWFKARVQIPREMAGRTVAAILNPGWEALCYL
                     NGAPAQGLDRNRSEVVLTTCAKGGEEFEIVIEASATPNQYDDHPIHQFTSAELAERDE
                     LVWSFYWDLKVANDVVAVLPPDSQPRMRLQDLVDWAVKLIDLNNKDDVASFRANIADA
                     QKRFRAKLKDFRRCPGDGKITYVGHSHIDTAWLWPLRETRRKCARTFSSALRYMDRYP
                     QFVFSQGQPQLYEFIKENYPTIWEGIKQRVKEGRWEATGGGWVEQDSNVAGAEALVRQ
                     YLYGRRFFIKEFGVQPRLVWLPDAFGFPITLPQIIAKCQLTAFGTTKINWSQYNQFPY
                     SHFLWRGLDGTEILAFMPPGSYNSNPTPANAVNDWRSVKQKDIVDELSITFGHGDGGG
                     GVTVEMFENVLRMKDVTGLPE"
     gene            complement(1466..>2955)
                     /locus_tag="P5179_13235"
     CDS             complement(1466..>2955)
                     /locus_tag="P5179_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRS96225.1"
                     /translation="ETISEAWKTVLLNQFHDILPGSSIAEVYEDAEKDYAGVRRALQN
                     LKDHSLRSLARRIDTRGEGDALIVWNSLGWKRDDVAITPLPSADSSGWSVLDPDGREV
                     ATQPVILESGAAGLLFEASDLPPMGYSVFRLVKRAPRVRGNLIARRNHLENRFFEVAL
                     SKTGSISRIFDKLNNREVLPEGSSANRLVLFEDFPASADAWDIDFNFEEKFQEIGDTE
                     SVTVTERGPVRATVRVVKRTKNSRITQDISIWASLSRIDFATEVEWYEKHKLLKASFP
                     VNVLSRIATYEIQFGAIERPTHRSGPVERARFEVPGHRWIDLSEADYGVSLMNDCKYG
                     FDVCENNMRISLLRGSVSPDPHADEGQHLFLYSLYPHRGDWRSALTTRRAYELNAPLS
                     CWIEKAHGGTHPRRWSFVSVDSSDIVIDTVKKAEDSDAIIIRVYESHGARGEATLQFG
                     KVPLNVTECDLMEEHDVDVPVKDSSVQLMMKPWEIKTLKATFSNG"
BASE COUNT          617 a          835 c          800 g          703 t
ORIGIN      
        1 attccggaag acccgtaaca tctttcattc tgagaacatt ctcgaacatt tcgacggtaa
       61 cgccgccccc tccgtcaccg tgaccaaacg tgatagacaa ttcgtcaacg atatccttct
      121 gtttgacgct tctccagtcg ttcaccgcat tcgccggagt cggattgctg ttgtaactcc
      181 ccggaggcat gaaggcgaga atttcggttc cgtcaagacc ccgccacagg aagtggctgt
      241 aaggaaactg gttgtactgg ctccagttga ttttggtcgt cccgaaggcc gtcaactggc
      301 atttggcaat gatttgcggc agagtaatcg gaaaaccgaa cgcatctgga agccacacga
      361 gacggggctg aacaccgaat tccttgatga agaaccgccg accgtacaga tactggcgaa
      421 ccagggcttc tgcacccgcc acgtttgaat cctgttcgac ccatccgccg cctgtggctt
      481 cccatcggcc ctctttcacg cgctgtttga tcccttccca gatggttggg tagttctctt
      541 tgatgaactc gtacagctgc ggttgtccct gggagaacac gaactgaggg taacgatcca
      601 tgtaccgcag cgcgctggaa aatgttcggg cgcacttgcg gcgggtttcg cgaagcggcc
      661 acaaccatgc ggtgtcgatg tgcgagtggc cgacataggt gattttcccg tctccggggc
      721 aacgacggaa atccttgagc tttgcgcgga accttttctg agcatcggcg atgtttgccc
      781 ggaatgatgc gacatcgtcc ttgttgttga ggtcgataag ctttacggcc cagtctacga
      841 ggtcctgcag cctcatacgt ggctgcgagt ctggcggaag aaccgccacc acgtcgtttg
      901 cgaccttcaa gtcccaatag aaggaccaca caagttcatc gcgctccgcg agctccgcag
      961 aagtgaattg atggattgga tggtcgtcgt attggtttgg tgtcgcggaa gcttcgatga
     1021 cgatttcaaa ttcctctcca cctttggcac aggtcgtcag caccacctcc gaacggttcc
     1081 tgtccaggcc ctgagccggt gcgccattca agtagcacag ggcttcccat cccggattga
     1141 ggatcgccgc gaccgttctt cccgccattt cgcgcgggat ttggacacgt gctttgaacc
     1201 actgggtaac gtcgtatcct ccccagtcat cgcccaattc gtacggtatc catccgtcca
     1261 caggaatggg ggcgggatat tgccccggac cggctacgta agtctgccat ccggtaatcg
     1321 gagtgcgatt gcgatacagc gctgcaaaaa gctcgttgag ccggttgccg atttgacgaa
     1381 cgagaatcgt cctttctgca ggaaaatggg gcatgctttc aggtcctttc aaaacgagag
     1441 tgttgtcatg ttatcgtgaa gacggtcagc cgttcgagaa tgtggctttc agtgtcttga
     1501 tttcccaagg tttcatcatc agttgaacac tcgaatcctt gacgggaaca tctacgtcgt
     1561 gttcttccat caggtcgcat tccgtgacat tcaggggcac cttgccgaat tggagcgtag
     1621 cttcaccccg ggcaccatgc gactcgtaca cacgaatgat gatcgcgtcc gagtcctccg
     1681 ctttcttcac ggtgtcgatc actatgtcag acgagtccac ggatacaaag gaccagcggc
     1741 gtgggtgcgt gcctccgtgg gccttttcta tccaacatga gagtggtgca ttcagctcgt
     1801 acgctcgtcg cgtcgtcaag gcggaacgcc aatctccgcg atgagggtac aggctgtaga
     1861 ggaacaggtg ttgaccttca tccgcgtgag gatctggcga gacggatccc cgaaggaggc
     1921 ttattcgcat gttgttctcg cagacgtcga acccgtattt gcaatcgttc atcaggctga
     1981 cgccatagtc cgcttccgag agatcaatcc acctgtgccc cggaacctca aaacgcgccc
     2041 tttcgaccgg gccagaacgg tgggtcgggc gttcgatggc tccgaactga atttcatagg
     2101 tggcaatgcg cgaaaggacg ttcaccggaa aagaggcttt cagcaatttg tgtttctcat
     2161 accactcgac ttccgtcgca aagtcgatcc gcgacagaga tgcccagatc gagatgtcct
     2221 gtgttatccg agagtttttc gtccttttca cgactcgaac ggtggcgcga acgggtcctc
     2281 gttctgtcac ggttacggac tctgtgtcac ctatttcctg aaacttctcc tcgaagttga
     2341 aatcgatgtc ccacgcgtcg gcgctggctg ggaaatcctc aaacagcacc agtctgttcg
     2401 ctgaagagcc ttccggaagg acttccctat tgttgagttt gtcgaatatg cgcgagattg
     2461 accccgtttt cgacagggca acttcgaaaa accggttttc cagatggttt cgccgggcaa
     2521 tcaaattccc ccgcactctc ggagcccgct tgacgaggcg gaaaacagag taacccatcg
     2581 gcgggaggtc cgacgcttcg aaaagcaatc ccgctgctcc ggattcaagg atcaccggtt
     2641 gggtggccac ctcgcgcccg tcggggtcca gtacactcca tcctgatgaa tcggcggagg
     2701 gaagcggggt gatggcgaca tcgtccctct tccatccgag ggaattccac actatcaacg
     2761 catcaccttc tccgcgcgtg tctatccggc gagcaaggct gcgcaatgag tggtctttga
     2821 ggttttgaag agctctccgc acaccagcat agtctttttc cgcgtcttcg tatacttcgg
     2881 cgatagacga gcccggcaat atgtcatgga actggttcag aagaacggtt ttccacgctt
     2941 cggagatcgt ctcct
//
LOCUS       DAOTNG010000363         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26363_length_2940_cov_4.484575, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000363 DAOTNG010000000
VERSION     DAOTNG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26363_length_2940_cov_4.484575"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="P5179_13240"
     CDS             complement(<1..416)
                     /locus_tag="P5179_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96226.1"
                     /translation="MSVERAHRAWAWVTGLMVSLGCVAVVASLFLPVLRDEGGYLFIA
                     DRVAHGAVYARDYFEWKTPLFFYLVAWLFRITGTSVFAARLAMLGCVAASAWLITGAA
                     RSAGIRPLWRAGAVALFLLLVPAFYSSHTLTEVPVMV"
     gene            complement(513..2819)
                     /locus_tag="P5179_13245"
     CDS             complement(513..2819)
                     /locus_tag="P5179_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96227.1"
                     /translation="MRPRILALFLAGVIAASAIPVRAQVLPPDSSVTVPAFTDIVAGL
                     QARLLALRDTASARGDSMLLRQVDLLSSELITAQAMFASGDILGAQVRISEIAAKVRV
                     LWSSLAPLPPPVPIPGDSVVILPPYPGPPLPPIEPVPVDIRELVQKAERLIVFLRGLA
                     TQQGDTTALAIIDRSEAIFRNLLASGTLADDEPTRATLWGIISTLERLLGQLQPLPPP
                     VPPDSSIVIVPPYPGLPYPPPDSVIIMPPFILPDSAAIAGRLDHIQAFLDSAVAVVRS
                     EPNLPASEMIPRAYAVVDSARDYLSEGAKQRAFDLATRAGILAGRIREAIAVRARVET
                     GVDLVRQHADSLALFLSLNPNARASEALAPVSAMLDSVRLLLDEGRVFRADAVLQRAR
                     EHLRLAHEMIALEAKLRRMLGELAQRIERVGPIVEASGDTVALALFERAKASADSAGK
                     ALDEGAYRRAGTLIEASNMALDRALFIATAPDRIAGEIAALRTRIARLLSSLPATAVR
                     ERAIVMRASALVDSAQVALNARLYPKAEQMLVKVRYALDTLLRTPIIDPVIPPVTTLP
                     LDSLVTLARQWVAQAVVLNDSARSDSVRVVLEQVVVLLDQVSAALADGNREEAVSLAN
                     NALILAASALRTARAELVDDWSSDPVVKDLIAAGEEAERAVQTPTEVDKGTTALQYTL
                     KNTPNPFNPSTTVRYTLGATERVNLAVYNMLGQEMRVLVRGVRSAGSHEATWDGRDAN
                     GIPLASGIYFARLQTESTTLVIRMVLSR"
BASE COUNT          601 a          963 c          908 g          468 t
ORIGIN      
        1 accatcaccg gcacttccgt cagcgtatgg gaggaataga atgccggcac aaggaggagg
       61 aagagggcta ctgccccggc tcgccagagc gggcgaattc cggcgctgcg cgcggcaccc
      121 gttatgagcc acgcacttgc ggccacacag ccaagcatcg caagccgggc cgcgaacacg
      181 ctggttcccg taatacggaa caaccaggca acgagataga aaaagagcgg cgttttccac
      241 tcgaagtaat cccgcgcgta caccgctccg tgggcgacgc ggtccgcaat gaagaggtag
      301 ccgccttcat cccggagaac cggcagaaaa agggaggcca ccaccgcgac acacccgagc
      361 gacaccatca atccggtcac ccacgcccac gcgcgatgag cccgctccac gctcatgcca
      421 tcccctcctg tgcccaaaca aaaacccgcc tgtgcaaaca tacgcacaca ggcggggtag
      481 gaaaaaccgt tccgagcgca acgcgggcgt ggttacctgc tcagcaccat ccgtatgacc
      541 agcgtcgtgc tctccgtctg caggcgggcg aaatagatgc cgctggcaag gggaatgcca
      601 tttgcatcgc gtccatccca ggtggcctca tggctgcccg cactgcggac gccacgtacc
      661 agcacgcgca tctcctgccc cagcatgttg tagaccgcaa ggttcacccg ctcggtggca
      721 ccgagggtgt acctcaccgt ggtggacggg ttgaacgggt tcggtgtgtt tttgagcgta
      781 tactgaagcg ctgtggtgcc cttgtccacc tctgtcggcg tctgcaccgc acgttccgct
      841 tcctcaccgg cggcaatgag gtccttcacg acggggtcgg agctccagtc atccaccagc
      901 tccgcccgcg ccgtgcgcaa cgcggaggca gccagtatca gcgcgttatt cgccagcgag
      961 accgcctcct cgcggttgcc gtccgccagc gcggccgaca cctgatcgag gagaaccacc
     1021 acctgctcga gaaccacacg cacggagtca cttctggccg aatcgttgag aacaaccgcc
     1081 tgtgccaccc attgccgggc gagggtcacc agactatcca gcggcaacgt ggttaccggc
     1141 ggaatcaccg gatcgatgat gggcgtgcga agcagcgtat caagcgcgta ccgcaccttc
     1201 acgagcatct gttccgcttt cgggtacagg cgggcattaa gcgccacctg agccgaatcc
     1261 acaagagccg acgcccgcat aacgatagcc cgttcgcgaa ccgcggtggc gggcaacgat
     1321 gacagcagcc gcgcgatacg ggtcctgagc gcggcgattt cccccgctat tcggtccggc
     1381 gcggtggcaa tgaagagcgc ccggtcaagg gccatgtttg aagcctcaat caacgttccg
     1441 gcccggcggt acgctccttc gtccagagcc ttccccgcac tgtctgcgga ggcttttgcc
     1501 cgctcgaaga gcgccagtgc cacggtgtcg ccgctggcct ccacaatcgg tccgacgcgt
     1561 tcaatgcgct gcgccagttc cccgagcatt cgccgaagct tcgcctcgag cgcgatcatc
     1621 tcatgcgcca gccgcaggtg ttcccgcgct cgttgtaaca ccgcgtccgc ccggaacacg
     1681 cgcccctcat cgagaagcag gcgcaccgaa tccagcatcg ccgacacggg tgcaagtgct
     1741 tctgacgctc gcgcattggg attcaatgaa aggaacagcg ccagcgaatc agcgtgttgc
     1801 cgaaccagat ccacgccggt ttcgacacgt gcccgcacgg caatcgcctc gcggatgcgt
     1861 cccgcgagga tacccgcccg cgtggccagg tcgaatgccc gctgttttgc cccctcggag
     1921 aggtaatccc gtgcgctgtc gaccaccgcg tacgcccgcg gaatcatctc cgaagcgggc
     1981 agattgggct ctgaccggac gaccgccacg gcgctgtcga gaaacgcctg aatatggtcc
     2041 agccgcccgg cgattgccgc ggaatccggc aggatgaagg ggggcatgat gatgacgcta
     2101 tccggcggcg gataggggag ccccgggtac ggcggcacga tgactatcga actgtcaggc
     2161 gggaccggcg gggggagtgg ctggagttgg cccagaagtc gttccagcgt gctgatgatg
     2221 ccccacagcg tcgcgcgggt gggctcgtcg tctgcaagag tcccactcgc gaggagattc
     2281 cggaaaatgg cttctgaacg atcaataatc gccaacgcag tggtatcgcc ctgctgcgtt
     2341 gcgaggccgc gcaggaacac gatgagccgt tctgccttct gcaccagttc gcggatgtcc
     2401 acaggaactg gttcgatggg cggaagcggc ggccctggat acgggggaag aatcacgacg
     2461 ctgtcgccgg gaatcggcac ggggggaggg agcggggcaa gtgaactcca cagcacgcgc
     2521 accttcgcgg cgatttcgga gatccgcacc tgtgcgccga ggatatcccc ggatgcgaac
     2581 atggcctgcg cggtaatcag ttcggaagaa agaaggtcaa cctgccggag aagcatgctg
     2641 tcgccccggg cgctggccgt atcccgcaac gcgagaagcc gcgcctgaag gcctgcaacg
     2701 atatcggtga acgcaggtac agtaacggag gagtccggcg gcagaacctg agcccgtacg
     2761 gggattgcgg aagccgcaat cacacccgca aggaaaagcg caaggattcg aggacgcatc
     2821 ggaagcctcc gcctgcaggg cactccagca cgttagaaag catacgcgtg cgcaccacgc
     2881 gtagtactct atttcgacaa tgtactctac ctccgaggca aaagcaattc gcgttccttc
//
LOCUS       DAOTNG010000364         2889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26944_length_2889_cov_4.889203, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000364 DAOTNG010000000
VERSION     DAOTNG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26944_length_2889_cov_4.889203"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="P5179_13250"
     CDS             complement(<1..1303)
                     /locus_tag="P5179_13250"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HRS96228.1"
                     /translation="MNQRPRRQILWVDDEIDLLRSHIIFLADRGYDVTPVTNGEDAVA
                     LASERSFDIVLLDEMMAGQDGLTTLDEIKSIHPTLPVVMVTKSEEEELMNEAIGKRID
                     DYLVKPVNPSQIVLACKRLLDARQIREQKLVRDYVSEFSNLHTQISTAGWSEWMDIFG
                     RLAAWDLEIHSYNDPSLSEMHHSQWRHCNQVFARYVEENYAGWVAAPRTGPITSAEVV
                     RQFVVPHVGARPVYFIVIDCMRLDQWLAIEPLIAEYFAVRREHYYATLPTATPFSRNA
                     LFAGMFPAGIARRFPDLWTPGNDEDQSLNRFENQLLVENLARVGVKQPVHYQKVLDAG
                     DGRAVLKKIGTWKNAGLVALVFNFVDHLSHGRSESEILQEITPDEASFRSLTVSWFSR
                     SALFDILKWISRTDSTVIVTTDHGSVLGTRATIARGNRETST"
     gene            1493..>2889
                     /locus_tag="P5179_13255"
     CDS             1493..>2889
                     /locus_tag="P5179_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRS96229.1"
                     /translation="MRSISLDGEWQLFPVPPTHPPITHPRELRSSGIPPIRGTVPGNV
                     ELDLLHAGVIPDPFFGTNVLRLKDFELHNWWYSRNFATPEFESGDRVVIRFEGVDTFA
                     TVWVNGNRAGSCDNMLIAHEFDITSLLRTGQDENELCVLLESPLTRAGSHRYEPSIAG
                     LPTNLEQAYVRKAPHMYGWDIMPRAVSAGLWRPVFLDVHRQPEIVDLFVRPYHVTADE
                     AAIQFRWQIQAEPTTWERCTVRVRGRCGASEFSHQESCRFIAGDFSVVVKKPQLWWPR
                     GYGDPALYDTVCELLLDGEVVDSRKLRVGIRTITLERTDVTLPDAPGEFVFRVNGQRI
                     LVKGSNWVPVDAFHSRDAARIPRIMDHVADVGCNMLRCWGGNVYEDHGFFDLCDQLGV
                     MVWQDFAMACGRYPLDDAFAAQLRKEAEFIVRKLRNHPSLAIWAGDNECDEAYAGAGL
                     DPNTNTLTRAVLPEVL"
BASE COUNT          596 a          838 c          830 g          625 t
ORIGIN      
        1 tggtgcttgt ttcccggttg ccccgcgcga tcgtcgctct cgttcccaga acgctcccgt
       61 ggtccgtcgt cacgatcacc gtcgaatcgg tgcgggaaat ccatttcagt atgtcaaata
      121 gcgcggaacg ggagaaccag gacacggtca gagagcgaaa tgaagcctca tcgggagtaa
      181 tttcctgaag tatctccgat tctgaccgcc cgtgggagag gtggtcaaca aagttgaaaa
      241 ccagcgcaac cagccccgcg tttttccatg ttccgatctt cttcagaacc gcccgaccgt
      301 caccggcgtc aagcactttt tgataatgga cgggttgttt gacaccgacg cgtgcgaggt
      361 tctcaacgag caactgattc tcgaatctgt tcagcgactg atcctcgtcg tttcctggcg
      421 tccacaggtc agggaatcgt ctcgcaatgc ccgcgggaaa cattccggca aacaacgcgt
      481 tgcgtgagaa cggagtcgcg gttgggagtg tagcgtaata gtgctcgcgc cgcacggcaa
      541 aatactccgc aatcagtggc tcgatggcga gccactggtc gagtcgcatg caatcgatca
      601 caatgaaata caccggacga gcgccgacat ggggaaccac gaactgccgt accacttcag
      661 ccgatgtgat cggccccgta cgcggcgcgg ccacccaccc ggcgtaattc tcctcaacgt
      721 accgcgcaaa tacctggttg cagtggcgcc actggctgtg gtgcatctcg ctgagggagg
      781 gatcgttgta agaatggatt tcgaggtccc acgcagcgag gcgcccgaag atgtccatcc
      841 actctgacca gccggccgtg ctgatttgcg tgtgaaggtt ggaaaactcg gacacgtaat
      901 cgcgcacgag cttctgttcg cggatctgcc tcgcgtcaag gagccttttg cacgcgagga
      961 caatctgact gggattcacc ggcttgacga gatagtcgtc tatgcgcttg ccgatggctt
     1021 cgttcatcag ctcttcttct tcactttttg tcaccatcac caccgggaga gtggggtgaa
     1081 tgctttttat ctcgtccagc gttgtcagac catcctgccc tgccatcatc tcgtccaaca
     1141 gaacgatgtc aaagcttcgt tccgaggcga gcgccaccgc gtcttcgccg ttggtcaccg
     1201 gtgttacgtc gtacccgcga tcggcaagga aaatgatgtg tgaacggagg agatcgattt
     1261 catcgtccac ccagagaatc tgccgccgtg ggcgctgatt cacgttctcc aactggcgta
     1321 cctccgacac cgtgtggtga tgcattccga gaaattacgt agcgaaatat acccttgtgg
     1381 acgcaccgga cgacagcgcc gcgggtgctg ttttgaacag catgcggccg ccgttatcct
     1441 ttcaacactc agagcccgga atcgtttcct accatgcttt ttcgaggtta ccatgaggag
     1501 catcagtctc gatggggaat ggcagctttt tcccgttcca ccaactcatc cgccgatcac
     1561 tcatccccgg gaactgaggt cctccgggat accgccaatt cgtggaaccg tccccggcaa
     1621 tgttgagctg gatttgctgc atgcaggcgt catacctgat ccctttttcg gcacgaacgt
     1681 acttcgcctg aaagacttcg aactgcacaa ctggtggtat tccagaaact tcgcgacgcc
     1741 ggagttcgaa tccggcgacc gtgtggtaat ccgttttgaa ggagttgaca cgttcgcaac
     1801 ggtatgggtg aatggaaacc gggccggcag ttgcgataac atgttgatag cacacgaatt
     1861 cgacataacc tctctgctgc gcacgggaca ggatgagaac gagctctgcg ttttgctgga
     1921 atcgcctctt acacgtgccg ggtcacaccg atacgaaccc tccattgcgg gccttcccac
     1981 gaacctggaa caggcatacg tgcgcaaagc tccccatatg tacgggtggg acatcatgcc
     2041 gcgcgcggtt tccgcaggcc tgtggcggcc tgtgttcctt gacgtgcacc gccagccgga
     2101 gatcgtcgat ctgttcgtcc gtccgtacca cgtaactgct gatgaagcgg caatccagtt
     2161 ccggtggcag atacaggccg aaccgacgac ctgggagcgt tgtacggtac gggtgcgcgg
     2221 cagatgcggt gcttcggagt tctcacacca ggaatcgtgc agattcattg ccggggattt
     2281 ctcggtagtc gtaaaaaaac ctcaactatg gtggccccgg gggtacggcg acccggccct
     2341 gtatgacacg gtgtgcgaac tgctgcttga cggcgaagtt gtggattccc gaaagctcag
     2401 ggtcggcatc cggaccatca cgctggaaag aacagacgtc actctgcccg acgcgcccgg
     2461 cgagttcgtg ttccgggtga atggccagcg catcctggta aaaggctcca actgggttcc
     2521 ggtggacgcg ttccacagcc gagacgctgc ccgcattccc cgcatcatgg accacgttgc
     2581 cgacgtcgga tgcaatatgc ttcggtgctg gggcggcaat gtgtacgaag atcacggatt
     2641 cttcgacctg tgcgaccaac tcggcgtcat ggtgtggcag gattttgcga tggcgtgcgg
     2701 acgttaccct ctggatgatg cgtttgcggc gcagttgcgg aaggaagctg agttcatcgt
     2761 gcgcaaactt cgcaaccacc cgtcgctcgc aatctgggca ggcgacaacg aatgcgatga
     2821 agcatacgcc ggggccggtc tcgacccgaa cacgaacacg ctgacgcgcg ccgtattgcc
     2881 cgaagtgct
//
LOCUS       DAOTNG010000365         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26956_length_2888_cov_5.258030, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000365 DAOTNG010000000
VERSION     DAOTNG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26956_length_2888_cov_5.258030"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="P5179_13260"
     CDS             complement(<1..>1174)
                     /locus_tag="P5179_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HRS96230.1"
                     /translation="PCHVTRKEVSFIAPEVPSVGYTTVAVHVVAGDRSEKAAPQAEEN
                     VLENEFWRITLDPQRGGGIVSLVDKETGRELVDREKGVGNDLIALTERGVRGGSPWEF
                     WTTGGRSYASERPASVRIERTCVGDSAVIHGELAGACSFTRTLTVRPNSRTIEAEVIL
                     HDYRRDDLMFVATFPAAIRGALPVVEDRFGTIVTKRNRTAFDYRTGGNHCYSDCVVTP
                     IYNWIASGWSARVDSGSAALNLGFMGLVIPHDEALDGAIEHLMSAFAHSGVTCTPYFD
                     DDDVPRRKPLEDIYSEPGFEKTKLENTLWNRVDDIALTNQWLAVDVEGNNAYLRSLFG
                     RLPENVEKRIRDVEGAEGWVLAIVEDDEVPHGWPPMPTLVLTASSPDALREAIRQIS"
     assembly_gap    1175..1274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1275..2842)
                     /locus_tag="P5179_13265"
     CDS             complement(<1275..2842)
                     /locus_tag="P5179_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96231.1"
                     /translation="MDLRRQFPEFPTALAYAATYLCADTDTDLLVSANGTEEYHWSTM
                     TLMLDGVEFGKNDSPALVRVPAGEHLFLLKIGGGVSGQYAWKIDVRMGVLQPLADGLG
                     AGLARLTGFWRGNGAKPRVEVEVVLANREDRILPVAAISAQVNGVEPGPSATDILLAP
                     RTVSSVRLSAPVGNHPQGATVGFSVRCGDRRVAAEGELPELPPDSILHVMQGFHCDPV
                     WLSDQHHYNLVSLDNVRQLLDACIADPSYRVFLHEIDYLKPFVDEYPDYRAVLFGMIQ
                     SGQVALGSSYNQPNEGNCSGEALIRNILYGCGFHRHFLGGRPRIYHAWDVFGHTPQLS
                     QIVAKSGLSGALWSKPIVGFPPLFRHMALDGTALPHIRTLYGWGTHSVDRLRESTTGL
                     VKEKASFGIKRHLVVDAGDFSCPTAWMVGKSAEMWDSLPKITMTGPEAFLKGVAEDGS
                     KLRVTSRTESQYHVGTVHSRPEMKIANRMGENLLTAAERWATFASLMGAIYPDRALDK
                     AWRQLLFAEHHDALS"
BASE COUNT          539 a          864 c          799 g          586 t
ORIGIN      
        1 ggctgatctg ccggatcgct tctcgaagtg cgtccgggct ggaggctgtc agcaccagag
       61 tcggcatggg cggccaccca tgcgggacct catcgtcttc gacgatcgcc agtacccaac
      121 cctccgcgcc ttccacgtcc cggattctct tttccacgtt ttcggggagt cttccaaaga
      181 gcgaccttaa gtacgcgttg ttaccttcaa catccacggc aagccactgg ttcgtaagcg
      241 cgatatcatc cacccggttc cagagagtgt tttccagttt cgtcttctca aagcccggct
      301 ccgaatagat atcctcaagc ggttttctcc ttggaacgtc atcgtcatcg aaataaggtg
      361 tacacgtcac gccgctgtga gcgaacgcag acatcaggtg ttcgattgct ccgtcgagtg
      421 cttcgtcgtg aggaatgaca agccccatga acccgagatt cagggcggcg ctgccagaat
      481 caacccgtgc agaccacccg ctcgcgatcc agttgtagat cggtgtcacc acgcaatcag
      541 agtaacagtg gttgccgcct gtgcggtagt caaaggccgt gcgattccgc tttgtcacga
      601 tggttccgaa tctgtcttcg accacgggaa gcgcgccgcg tattgcggca gggaaagtcg
      661 ctacgaacat caggtcgtcg cgccggtagt cgtggagaat tacctcagct tcaatggtcc
      721 ggctgttcgg cctcactgtc agcgtacggg tgaacgaaca cgcaccggcc agttcgccgt
      781 gtattaccgc ggagtctccc acgcaggttc gttcgatccg aaccgaggcc ggtcgctccg
      841 aggcgtagct ccttccgcct gttgtccaga attcccacgg cgatccaccc ctgacgccgc
      901 gttcagtgag cgcgatgagg tcgtttccta cccctttttc gcgatccacg agctcccgcc
      961 ccgtctcttt gtcaacgagg ctcacaattc caccgccgcg ctgcgggtcg agagtgattc
     1021 gccagaactc gttttcgagc acgttctctt ccgcctgtgg ggcggctttt tcgctgcggt
     1081 cgccagccac gacgtgaaca gccaccgtgg tgtatcccac actcggtact tcaggagcga
     1141 tgaatgagac ctccttccgg gtaacgtggc acggnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnngacaac gcgtcgtgat gctccgcgaa aagaagctgg cgccacgcct
     1321 tgtcgagggc cctgtcagga taaattgcac ccatcagaga ggcaaaggtt gcccagcgtt
     1381 ctgcggctgt cagcagattc tctcccatcc ggttcgcgat tttcatctcc gggcgtgagt
     1441 gcaccgttcc gacgtggtac tgggattccg tcctgcttgt gacgcggagc tttgacccgt
     1501 cttcagcaac gcctttcagg aaagcttcgg gtcctgtcat ggtgattttc ggcaaggagt
     1561 cccacatttc agcgcttttg cccaccatcc aggcagtggg gcaactgaag tcaccggcgt
     1621 ccaccaccag atggcgcttg atgccgaagg atgccttttc cttcaccaat ccggtcgtgg
     1681 attcccgcag gcggtctacg ctgtgcgtgc cccagccgta gagcgtacgg atgtgcggca
     1741 acgcggtgcc gtccagagcc atgtgccgaa acagtggtgg aaatccgacg atgggtttgg
     1801 accacagagc tccgctcagg ccagattttg cgacgatctg gctgagctga ggggtatgcc
     1861 cgaatacgtc ccacgcatgg taaatccttg gcctgcctcc gaggaagtgc cgatggaatc
     1921 cgcacccgta aaggatgttc cggatcagcg cttcgcccga gcagttgcct tcgttgggct
     1981 ggttgtacga gctcccgaga gcaacctgcc cgctctggat catcccgaac aggaccgcgc
     2041 ggtaatccgg gtattcgtca acaaacggtt tcaggtagtc tatctcgtga agaaacacgc
     2101 gataactggg atccgctatg caggcgtcca gaagttgcct gacgttgtcg agggagacga
     2161 ggttgtaatg gtgctgatcg ctcaaccaga cgggatcgca gtggaatccc tgcatcacat
     2221 gcaagatcga gtcagggggc aattcgggaa gttcgccttc cgccgctacc cttctatccc
     2281 cgcagcggac cgagaagccc actgtggcac cctgaggatg attaccgacg ggtgccgaaa
     2341 gcctcacaga cgagaccgtg cgtggcgcaa gcaggatatc cgtcgccgaa ggcccgggtt
     2401 cgaccccgtt cacctgggcc gaaatggcgg cgacgggcaa gattcggtct tcccgattgg
     2461 cgagcactac ctcgacttca acgcgtggct ttgcaccgtt tcccctccag aatcccgtca
     2521 gtcgggcaag accggccccc agcccatccg caagcggctg caaaaccccc atgcgaacat
     2581 caattttcca tgcgtactgt ccactgacac ctccaccgat tttcaacagg aaaaggtgct
     2641 ctccggcagg gacacgcacc agcgccgggg aatcgttttt cccgaactcg actccgtcca
     2701 gcatgagcgt catagttgac cagtgatact cctccgtccc gttggccgaa acgagcaggt
     2761 ccgtgtctgt gtcggcgcac aggtatgtgg cggcgtacgc aagggcggtg gggaattccg
     2821 ggaattgacg tctgagatcc accgcgggag tgccgctgca cctgcgccag gtggcgcacc
     2881 cggccgga
//
LOCUS       DAOTNG010000366         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_26991_length_2885_cov_5.531802, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000366 DAOTNG010000000
VERSION     DAOTNG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26991_length_2885_cov_5.531802"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..2360)
                     /locus_tag="P5179_13270"
     CDS             complement(270..2360)
                     /locus_tag="P5179_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96232.1"
                     /translation="MRLLLSFLTVALFAPAAHSANVSGTISQNTTWTKANSPYVVTGA
                     ITVATGVTLTIEAGVVVYFGGWYPFTVQGRMNANGTAKDSVLFVQEPRSDTWLGLEFL
                     GSDSSTLSYTRISNVEEDDTGGAVNVGGTARLRLTHCVIAYNSGINGGAIFNGDGSES
                     SIVYADWTVMHSNNAYGEGSAVYTSNTAKTYLTNCTITKNTSGTSGAAVYSNAGTVTM
                     LNCIVWNSGTSVTVAGGGTVTATYSDIQGTTVFTGKGNINADPKFLAPARNIFLLQSS
                     SPCVGAGAGGEVMGAFIFVPTTLTILQPSAMPGDTLLLTIRGTFLPALAVDVAFRINT
                     AAIMPDTVGKSLVSAHAFSSAADGAVWYNIVADTVFLSFSATERVGLANQALVSLRFR
                     VAPGAAGGDYPATFVASETNIEEFAVDNLNNGKVYVQTLGDVTKTSGLTAADATEILK
                     FVVRLRPTIDINLADVTCNGKVTSFDAAWVLQKVLHPEYLFPCEGGGPLPRPAGHPAL
                     VLRWQREGDAWLLFGDHAGTLAGDLTLALGSEDAVSATGSGTVVANQDGSVLHVGLVD
                     FSGEPLLLRIEGAGASAQPLILAADINDGEYRISGVRPVALALEQNVPNPFNPSTTIQ
                     FRVPDAGFVSVAIYGIDGQLVRSLVNRSVEAGPHEVVWDGRDNAGRAVASGVYVYRLT
                     SDRGVISRRMVLAR"
     gene            complement(2662..>2885)
                     /locus_tag="P5179_13275"
     CDS             complement(2662..>2885)
                     /locus_tag="P5179_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS96233.1"
                     /translation="GDHAAKWETSLMLYLRPDLVDMSALDPDPAKKPVGVLGVDPRGN
                     ASAEYGKQGMEAMTEALRPKVLEALAAMQ"
BASE COUNT          573 a          877 c          852 g          583 t
ORIGIN      
        1 gggttcgcga ttgtctgtta cgtgcagccg agcgcggtca atatcctgct tgccgtgctg
       61 tttctcgcca gcgcaggttt tcacggctgg cgaatcgtga aacggatccg agcagcggct
      121 tcctcttcct gcaacgatcc aacgcatcac cactcgagct gaaagccgtt cgcgggccga
      181 gagcctctcc tcagcacgaa aaaacggggg cgacgttgca gtcgcccccg ttgatgccgc
      241 gaaattcgtc ccgcggtgca ggtggtttcc taccgcgcca gcaccattct ccttgatatc
      301 acaccccgat ccgaagtcaa gcgatacacg tacactccgc tcgccacagc ccggcctgcg
      361 ttgtcgcgtc catcccacac gacctcgtgc ggacccgctt ctacggaccg gttgaccagt
      421 gaacggacga gttgtccgtc aatgccgtaa atcgccacac tgacgaaccc tgcgtccgga
      481 acgcggaact ggatcgtggt ggaggggttg aacgggttcg gtacgttctg ctcgagcgcc
      541 agcgccaccg gacggacgcc ggaaatcctg tattcaccat cgttgatgtc ggcggcaagg
      601 atcaacggtt gtgccgatgc gcccgctcct tctatgcgca gtagcaacgg ctcgccggag
      661 aaatcaacca gcccgacgtg cagaacgctg ccgtcctgat tggcgaccac ggtgccgctg
      721 cccgttgcgc tgactgcatc ctccgaaccc agcgccagcg tcaggtcacc cgcaagtgtt
      781 ccggcatggt cgccgaagag aagccacgca tcgccttccc tctgccagcg caggaccagc
      841 gcagggtggc ccgccggacg gggaagaggg cctccacctt cgcacgggaa gagatactcc
      901 ggatgcagca ccttctggag cacccatgcg gcgtcgaaac tcgtgacctt cccgttgcac
      961 gtgacatccg ccagattgat gtcaatcgtc ggcctgaggc gtaccacgaa cttgagaatc
     1021 tccgttgcgt cggcggcggt aaggccgctg gtcttcgtca cgtcgcccag cgtctgaacg
     1081 tacaccttgc cgttgttgag attgtccacg gcgaattctt caatgttggt ctccgacgcg
     1141 acgaatgttg cggggtaatc gccacccgcg gcaccggggg caacgcggaa tcgaaggctc
     1201 acaagtgcct ggttggcgag cccgacgcgt tcagtcgcgc tgaacgacag gaataccgtg
     1261 tcagcgacga tattgtacca gacagccccg tccgccgcag atgaaaacgc gtgggcagag
     1321 acgagcgatt tccccacggt gtcgggcatg atggcggcag tgttgatacg aaacgcgacg
     1381 tccactgcaa gcgcgggaag aaaggtgccg cggatcgtca agaggagtgt gtcgcccggc
     1441 atagcggaag gctgcaggat ggtcagggta gttgggacga atatgaatgc ccccattacc
     1501 tcgccgccgg cacctgcccc aacgcacgga gaggaggact gaagcaagaa gatgtttcgc
     1561 gcgggagcca ggaatttcgg gtccgcgttt atgttccctt tcccagtgaa tactgtcgtg
     1621 ccctggatgt ccgaatacgt ggctgttacc gttccgcctc cggcaacggt tacagatgtc
     1681 ccgctgttcc acacgatgca gtttaacatc gtaactgtgc ctgcgttgga gtataccgcc
     1741 gcgccgctgg tccctgaagt gtttttcgtg atggtgcagt tggtgaggta ggttttcgca
     1801 gtgttgcttg tgtaaaccgc actcccctcc ccgtaagcgt tattgctgtg cattacggtc
     1861 cagtcggcgt acacgatcga ggattcgctg ccgtccccgt tgaaaatggc gccgccgtta
     1921 attccggagt tataggcgat gacgcagtgg gtgagccgca ggcgcgcagt gccgcctacg
     1981 ttcacggcgc cgccggtgtc atcctcctcc acgttgctta tcctggtgta ggacagggtg
     2041 ctggagtcgc tgcccaggaa ttccagtccc agccacgtgt cagacctcgg ttcctgaaca
     2101 aacaaaacgg aatccttggc ggtgccgttt gcgttcattc tcccttgaac cgtgaacggg
     2161 taccacccac cgaaatacac gaccacgccg gcttcgattg tcagggtcac gccggtggcg
     2221 accgtgatcg cccctgtcac cacgtacggg gagttggctt tggtccatgt ggtgttttgc
     2281 gagatagtgc cagaaacgtt ggcagagtgt gccgccgggg cgaacaatgc tacggtgagg
     2341 aacgaaagaa gtaaacgcat ggtggcctcc cttcacgcgt acgcgcaaaa aaatcgtgca
     2401 agaacagtgc gggagaagcc agatactccc gcgcagtaca ccctgttccg gcccgaatgg
     2461 gaccggttta tcaccaattt ttcaccaaaa ctgatcccga gcgcaaaagg agacaggcgg
     2521 aacgcgttac accgtcgtgg aaaaaagact tatctgtgcg cgccgaatgt actcgcctgc
     2581 aattacaaaa atatgctgct tcaaaaagct gtcaactcag tggtaacaag aattgtaccg
     2641 tcgagcggtg gcatcgctcc ctcattgcat cgccgccagt gcttcaagca ccttcgggcg
     2701 cagggcctct gtcatggctt ccatgccctg ttttccgtat tccgcggagg cattgccacg
     2761 cggatccacg cccagaacgc ccaccggctt cttcgcgggg tcggggtcga gcgcgctcat
     2821 gtccaccagg tcgggccgca ggtagagcat gagtgaggtt tcccatttcg ccgcgtggtc
     2881 gcctt
//
LOCUS       DAOTNG010000367         2884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27004_length_2884_cov_5.132556, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000367 DAOTNG010000000
VERSION     DAOTNG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27004_length_2884_cov_5.132556"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..647)
                     /locus_tag="P5179_13280"
     CDS             complement(21..647)
                     /locus_tag="P5179_13280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRS96234.1"
                     /translation="MPRLNIPATKINLIRIKDELKLAVDGHELLEQKRQVLIMEVRKL
                     INSLRAERSEMENLLREAYAKLAQATWENGAEQVALAGLATSQAIDISLRERSVMGII
                     LPVVDATEAELRPSYSLSGTTSSLDEAARAFHGALQHIARLAELENAALRLATELMKT
                     QRRVNALKNFFIPQYHETVKFLQETLEERERDSLFALKRLKSKADLPA"
     gene            complement(720..2105)
                     /locus_tag="P5179_13285"
     CDS             complement(720..2105)
                     /locus_tag="P5179_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRS96235.1"
                     /translation="MATEIGKEYVGVQRIVGPLVMIEGIDGVGYDEMVEIVSDDGKLK
                     HGRVLEVGTKAAVVQVFEHTTGLSIGATKMKFLARPVRIPVSEEMLGRTFNALAEPID
                     GGPPPIPKDLRPVSGAAINPTLRHHPSDFIQTGISAIDAMSTLVRGQKLPVFSASGLE
                     HDRVIAQIIRQAKIIGKQEPFAVVFGAMGLKHDIADFYRKSFEESGAGNNVATFLTLA
                     DDPSIERIITPRSALTLAEYLAFDVGMHVLVVLTDMTNYCEALREVATAKGEVPSRKG
                     YPAYLYSDLAALYERAGQVEGRTGSITQLPLLTMPNEDITHPIPDLTGYITEGQIVLS
                     KALQGAGIYPPINVLPSLSRLMNDGIGEGRTREDHRNVSSQLYAAYARVKRVEVLAAV
                     IGEEELSEIDKQYLTFGQHFEKEFIQQAPDEDRSIEETLNLGWKLLKYLPVSELTRVK
                     EEQIAKYLPKD"
     gene            complement(2116..>2884)
                     /locus_tag="P5179_13290"
     CDS             complement(2116..>2884)
                     /locus_tag="P5179_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRS96236.1"
                     /translation="SRWAEALRELSGRLEEMPAEEGFPAYLATRLAEFYERAGMVETL
                     GGSTGSVSVIGAVSPAGGDFSEPVTQHTSRFIGCFWALDKELASARYYPSINYINSHS
                     AYTEFVADWWKKIGGEKYNWEAMREETVDVLRRDDKLQQIVKLVGEEELPDEQKLILD
                     TARILQNAFLQQSAFDEVDTYSVPEKQLRMLSVILHYYHRGSDVVKRSIPIYKYQQLP
                     VRNEIMRMKSRIPNDKLEEFDTLERAIDEQIDALIAE"
BASE COUNT          550 a          862 c          769 g          703 t
ORIGIN      
        1 gccaagttcc cttgcgccgg tcaggcaggg aggtcggctt tcgacttgag gcgcttcaac
       61 gcgaaaaggg agtcgcgttc gcgttcttca agcgtttctt gaaggaattt cacggtttca
      121 tggtattggg ggatgaagaa gttcttgagt gcattaaccc gtcgctgggt tttcatcagt
      181 tcagtggcca gccgcagagc cgcattttcc agttccgcaa gtcgtgcgat atgttgcagc
      241 gctccgtgaa acgcacgggc agcctcatca agtgagctcg ttgttcccga aaggctgtac
      301 gagggccgaa gctccgcctc cgttgcgtct accaccggga gaattattcc catcacgctg
      361 cgctctcgca ggctgatatc aatagcctga gaggtcgcca gacctgccaa cgcgacttgc
      421 tccgcgccgt tctcccacgt tgcctgtgcg agtttcgcgt acgcttcgcg caagaggttt
      481 tccatttcgc tgcgttctgc tctcagacta tttatgagct tccttacttc catgatcagc
      541 acctgccgct tctgctccag cagctcgtgg ccgtccacgg ccagtttcaa ctcatccttg
      601 attcggatga ggttgatctt tgtcgccgga atgttcaaac gcggcatttc aatggctcct
      661 cacgcggctg atcacgtctc cagtacggtg tgtttggttt ccagtcaaaa ggaccgtctt
      721 caatccttcg gcagatactt cgcgatctgt tcttctttca cgcgggtaag ttccgaaacg
      781 gggaggtact tgagcaactt ccacccgagg ttcagtgtct cttcgatgga gcgatcctcg
      841 tccggcgcct gttgaatgaa ctccttttca aaatgctgcc cgaaagtgag gtactgcttg
      901 tcgatctccg acagttcttc ctcgccaatg acggcagcaa gaacctcgac gcgttttact
      961 cgcgcatacg ccgcatagag ctgactcgat acgttcctgt ggtcttcccg ggtgcgacct
     1021 tcgccaatcc cgtcgttcat gaggcgggaa agcgacggaa gcacattgat cgggggatag
     1081 atccctgccc cctgcaatgc cttggagaga actatctgac cttccgtgat gtaccccgtg
     1141 aggtcgggga tcggatgcgt aatatcctcg ttaggcatcg tcagcaaagg aagctgagtg
     1201 atggaacccg tccgcccttc gacctgaccc gcacgttcgt acaatgcggc aagatcgctg
     1261 tacaggtacg cggggtaccc ttttcgactg ggcacttccc cttttgccgt tgccacctcc
     1321 cgcagggctt cgcagtaatt cgtcatgtcg gtaagaacca ccaggacgtg cataccgacg
     1381 tcaaaagcga ggtattccgc aagcgtcagc gcggatcggg gcgtaatgat ccgctcgatt
     1441 gagggatcat ccgcgagtgt cagaaatgtg gcgacgttgt ttcccgctcc gctctcctca
     1501 aagctcttgc ggtagaaatc cgcaatgtcg tgcttcagac ccatcgcgcc aaacaccacg
     1561 gcaaacggct cctgtttgcc gatgattttc gcctgacgaa tgatctgggc aatcacccga
     1621 tcgtgttcca gtcccgacgc tgagaatacg ggcagcttct gcccacgcac gagcgtgctc
     1681 attgcgtcga tggcagagat accggtttgt atgaagtcgc tcgggtggtg acggagcgtc
     1741 ggattgattg ctgcgccgga aaccgggcgc aaatctttcg gaatcggtgg cggcccgccg
     1801 tcaatcggct ccgccagcgc gttgaatgtg cgcccgagca tttcctcgga aaccggtatt
     1861 cggaccgggc gggcgaggaa tttcatcttc gtcgcgccga tggagaggcc cgtcgtgtgt
     1921 tcaaatacct gtacgacggc ggccttggtg ccgacttcga gcacgcgccc gtgtttcaac
     1981 ttgccgtcgt ccgagacaat ctccaccatc tcatcgtacc cgacgccgtc gataccctcg
     2041 atcatgacca gagggccaac tatgcgctgt acgccgacgt attccttgcc aatttctgtc
     2101 gccatgggtg tggcctcatt cggcgataag tgcgtcaatc tgttcgtcta tcgctcgttc
     2161 gagcgtatcg aattcctcca gcttgtcgtt cggaatccgc gatttcatgc gcattatttc
     2221 gttccgcacc ggaagctgct ggtatttgta gatcgggatg cttcgcttga cgacatcgct
     2281 tccacgatgg tagtaatgca ggatgacgct cagcatccga agttgcttct ccggaacgga
     2341 gtacgtatcc acctcgtcaa acgcgctctg ttgcaggaac gcattttgaa gtatccgcgc
     2401 ggtgtcgagg atgagtttct gttcatccgg cagttcttct tcaccgacaa gtttcacgat
     2461 ctgctgcaat ttgtcatcgc gacgcaggac atccaccgtc tcttcccgca ttgcctccca
     2521 gttgtacttc tccccaccga tcttcttcca ccagtcagca acaaattccg tgtacgcact
     2581 gtggctgttg atgtaattga ttgatggata gtaccgcgcg cttgccagtt ccttgtccag
     2641 cgcccagaag caaccgatga aacgggacgt atgctgggtt accggttcgc tgaaatctcc
     2701 acctgcgggc gacacagcac cgatcacact cacagatccc gtgcttcccc cgagcgtctc
     2761 gaccattcca gcgcgttcgt agaactccgc cagccgtgtc gccaggtatg cagggaaccc
     2821 ctcctccgca ggcatttctt ccagccggcc ggacagctca cgcagcgctt cagcccatcg
     2881 ggac
//
LOCUS       DAOTNG010000368         2867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27216_length_2867_cov_6.108464, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000368 DAOTNG010000000
VERSION     DAOTNG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27216_length_2867_cov_6.108464"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="P5179_13295"
     CDS             <1..811
                     /locus_tag="P5179_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS96237.1"
                     /translation="LAHSIGGVVAAPLWQMLFEVFDTRFPTESALQLVFLAAACVCAA
                     AGALFFAFPLPTQFAGRKTPAAAEPGDRASLWGLPLVVVCAFAALQAGAYNSAFGWIP
                     DFATRTFAPNPFPVSWILSGISLSYVVGRLILLRLPERLPDLRVIFVCASTGALLFGA
                     AFGSTNQYTMGILCVVGGLVLSADWPSIFSHTARLFPVHAGKAIAMAGASAGVMSFVL
                     PPFMGFIAEQTGSLRAGMMVPPLLFGVLSVSALSWERYLRRARKHPLHTES"
     gene            897..1319
                     /locus_tag="P5179_13300"
     CDS             897..1319
                     /locus_tag="P5179_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRS96238.1"
                     /translation="MRKPGTKVLISLGLIAAAIAILVVAATMRQGATIHYYTTTSEFL
                     NRSPDYINKPVRVNGKVVPGSLERVGSDGPTDLRFALGDSVHHTLPVHYRGTALPDAF
                     REGADVVVEGVYLPTGVLEARQILAKCPSKYEAAPANR"
     gene            1432..>2867
                     /gene="ccsA"
                     /locus_tag="P5179_13305"
     CDS             1432..>2867
                     /gene="ccsA"
                     /locus_tag="P5179_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRS96239.1"
                     /translation="MAEIGHYSLLIAFFLALYSAIASFAGTRAPELGESAKRAALALF
                     WLLTIAAVILVYLLAVRDLTVEYVARYTSTVLPMSYAISAFWAGNAGSLLFWVWLLSA
                     YAAVVVWRNRKSPSRLVIPALGVMMLIAVFFLGLILFLYRIDPEMTPGMVRDPFYATL
                     QALFGSPEGGLHPACNPFWRLPFRPAEGLGLNPQLQNPGMMFHPPTLYLGYVGMAVPY
                     AFAMAALLTGRVGNEWLSLTRRWTLVAWFFLSIGNILGGWWAYVTLGWGGYWGWDPVE
                     NAAFIPWLLATAFLHSVIMQEKRGMFRTWNMVLVLITFVSTIFGTYLTRSGVISSVHA
                     FARNEAFNIAFLAFMAVACVVSFAFLFVRRDQLVSESPIESVFSREAAFLFNNVVLVG
                     IAFVVLFGTVFPAISSAFTGVQVSMGPAWFNSVLWPLWAMLLVLTGVGPVISWRRARR
                     NEFQRGFAIPSTLGIVVAAGLALAGMRNG"
BASE COUNT          454 a          887 c          855 g          671 t
ORIGIN      
        1 gctggcccat tccattggcg gcgtggttgc ggcccctctc tggcagatgc tgtttgaagt
       61 attcgacacc cgtttcccga ccgaatctgc gcttcagctt gtgttcctcg ccgccgcctg
      121 cgtgtgcgcc gcggcgggcg ctctcttttt cgcgtttcct cttccaacac agtttgcagg
      181 cagaaagacg cctgcagcag ccgaaccggg agatcgggcg tcactttggg gcctcccgct
      241 ggttgtggtc tgcgcgttcg cggcacttca ggccggggct tacaattcag cgttcgggtg
      301 gataccagat ttcgccaccc gaaccttcgc cccgaacccc ttccctgttt cgtggatcct
      361 gtccgggata tcgctttcct acgtcgtcgg aaggctcatc ctgctccgcc ttccggagcg
      421 gttgccggac ctgagggtta ttttcgtctg cgccagcacg ggagcactgc ttttcggtgc
      481 cgcgttcggc agcaccaacc agtacaccat ggggatcttg tgcgtcgttg gaggcctggt
      541 tctctccgcg gactggccct cgattttctc gcatactgcc cgcctattcc ctgtacacgc
      601 cggaaaggca atagccatgg cgggagcttc agcgggcgtc atgtcgttcg tgctgccgcc
      661 gttcatgggg ttcattgccg agcagacagg atccttgagg gccgggatga tggtgccgcc
      721 tctgcttttc ggagtgcttt ccgtctctgc gttatcctgg gaaagatatt tgcgccgcgc
      781 gcggaaacac ccgcttcata cggaaagttg agctgcggga ttccggttca cgcaacgccg
      841 tttcgtacat ttcttgcgcc aaaacacgcc ggaatgacgt tagcaggaaa tacacaatgc
      901 ggaaaccggg tacgaaggtc ctgatcagcc tcggtctcat cgccgcggca atcgctattc
      961 tggtggttgc cgccacgatg cgccagggcg ccaccattca ttactacacc accacctcag
     1021 agttcctgaa ccgctcaccg gactacatca acaaacccgt gcgcgtaaac gggaaggtgg
     1081 tgccgggttc acttgaaaga gtggggagcg atggacctac ggacctcaga ttcgcgctgg
     1141 gggactccgt gcaccacact ctgccggtcc attaccgggg caccgcgctt cccgacgcgt
     1201 tccgggaagg ggctgatgta gtggtcgaag gggtgtacct gccgaccggc gtgctggaag
     1261 cgcggcagat tctcgcgaaa tgcccgtcca agtacgaggc tgcacctgcg aacaggtagc
     1321 atcgggatac ggcacattga aggttcccgc ggtacattta ctgccctgta taccagacca
     1381 ctgcgccgcg atcgccgaag caccatcttg ccccgttgaa acgagacctg tatggcggaa
     1441 atcggtcact actcccttct catagccttc ttcctcgcac tctactccgc gatcgcctca
     1501 tttgcgggca ctcgtgctcc ggaactcggc gaatccgcaa aacgcgctgc gctcgcgctg
     1561 ttctggctgt tgacgatcgc cgcggtgatc ctcgtatatc tccttgccgt gcgggacctg
     1621 accgtcgagt acgttgcgag gtacacgagc accgtcctgc ccatgtcgta cgccatctca
     1681 gcgttctggg ccggcaacgc gggatcgctg ctgttctggg tgtggctcct ctccgcgtac
     1741 gcggctgttg tggtgtggag gaaccggaag tcgccatcgc ggcttgtcat tccggcactc
     1801 ggtgtcatga tgctgatcgc agtgttcttc ctcggactca tcctgttcct ctacaggatt
     1861 gaccccgaga tgaccccggg catggtgcgc gacccgttct acgctaccct gcaggctctt
     1921 ttcggcagtc cggaaggagg cctccatccc gcatgcaatc ccttctggcg cctgccgttc
     1981 cgccccgcag aagggctcgg gctgaatcct cagcttcaga accccgggat gatgttccat
     2041 cctccgactc tttacctcgg atacgtgggg atggccgtcc cgtatgcctt cgcgatggcg
     2101 gccctcctca ccgggcgcgt gggcaatgaa tggctttcgt tgacacgacg gtggacgctg
     2161 gtcgcctggt tcttcctttc catcggaaat atcctgggtg gctggtgggc gtatgtcacg
     2221 ctcgggtggg gtgggtactg ggggtgggat ccggtggaaa acgcggcatt catcccctgg
     2281 ctgctcgcca cggcgttcct ccactcggtt atcatgcagg aaaagcgtgg aatgttccgg
     2341 acgtggaaca tggtgctggt tctgatcaca ttcgtttcta ccattttcgg cacgtacctc
     2401 acacgcagcg gggtgatctc ctccgtgcac gccttcgcgc ggaatgaagc attcaacatc
     2461 gcgttcctgg cgttcatggc cgttgcatgc gtggtgtcgt tcgcctttct gttcgtccgt
     2521 cgcgatcagc ttgtgtcgga aagtccgatt gaatccgtgt tttcccgcga agccgcattc
     2581 cttttcaaca acgtggtgct ggtcggcatc gcgttcgtcg tcctgttcgg aaccgtcttc
     2641 ccggcgatca gcagcgcgtt caccggtgtg caggtcagta tgggaccggc gtggttcaac
     2701 agcgtcctgt ggccactgtg ggcgatgctc ctcgtgctga cgggtgtcgg cccggtgatt
     2761 tcctggcgtc gcgcacgcag aaacgagttc cagcggggtt tcgcaatccc gtccacgctg
     2821 ggaattgtgg tggcggcagg ccttgctctt gcaggcatgc gcaatgg
//
LOCUS       DAOTNG010000369         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27376_length_2854_cov_5.492676, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000369 DAOTNG010000000
VERSION     DAOTNG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27376_length_2854_cov_5.492676"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="P5179_13310"
     CDS             <1..865
                     /locus_tag="P5179_13310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRS96240.1"
                     /translation="RDGFAGLISDEIIPMTSPSVGGIVGRGGTILGTARCEEFRDPAG
                     RAKAAETLRKHGIEGIVVCGGDGSFRGAHLLWKEHNIPVICTPGTIDNDLAGTDYTIG
                     FDTAVNTALEAIDRIRDTAEAHRRTFIVEVMGRHSGFIALATAIGGGADAVLVPEIPP
                     DIPRIVSMVERRFAAGKRFSLIVVAEGVVIDDFPGAGSLAHILKKHGLDCRVSILGHQ
                     QRGGAPTALDREFASLLGKEAVEELLRGGTDRMAGIVGKRVEFCDLEFSWREKKPIDM
                     EVYRFISEAAI"
     gene            1062..2159
                     /locus_tag="P5179_13315"
     CDS             1062..2159
                     /locus_tag="P5179_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRS96241.1"
                     /translation="MARRITIADIARYAGVSPATVSLVLNGKYTQGQTARGTAERVWE
                     AARVLGYRGRIRMRPEPGAPAIGLLIADRESFFTTYYGPRLLKGMWHVADEYNAHLFL
                     FSDRMEGQPVTFDYLRKELESRRVNDLVVNVNNNLSESLLDTVEALAIGGTRIVALWR
                     RPKSWFAPCVAPDDENAALLMLSHLFELGHRCVALVATEPDERTLLITERFAAAAREM
                     GVELAFGEALFENGGKWGVLDDDQAVARLLGCSSPPTAIATLYDGRAVGVTKALGSLG
                     YRVPDDISLIAYGNMPENRPTPYLITMVQSPIEQIGETAVRLLVDPESSYSACCAGPP
                     IRLPVALRVGATTGPASKAGPYSRSRVLRSF"
     gene            2225..>2854
                     /locus_tag="P5179_13320"
     CDS             2225..>2854
                     /locus_tag="P5179_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96242.1"
                     /translation="MNSLRAVWMAIAAVFLANAADSRESATIRITVGPDRSDWPHAPV
                     AIRLDSILSRNGWKSPLGNSLIRVRESGNARLVPSRAGWNHSGECPGLLLFSKTGPGR
                     REYVITVTSDEATPAGERIPVVGFGEPLTHGVTGIVSGIDGSFNSNLAVADWDGDGDL
                     DFFVAQGQGGGANWTMHGLLYYENVGTSRSPLMAKPRRLFESTSLPLVAD"
BASE COUNT          559 a          771 c          913 g          611 t
ORIGIN      
        1 ccgggacggg ttcgccgggc ttatttccga cgagatcatc ccgatgacgt cgccgtccgt
       61 cggtggcata gtcggcagag gtggtacgat actcggaact gcccgttgtg aggagtttcg
      121 cgatcccgcc ggccgtgcga aagccgcgga aacattgcgc aaacatggca tcgaagggat
      181 cgtggtctgt ggcggagacg ggagtttccg cggtgctcat ctcctgtgga aagagcacaa
      241 cattcccgtc atatgcacgc cgggaacaat agacaacgac ctcgcgggaa ctgactacac
      301 catcgggttc gacactgcag tgaacaccgc gctggaggcg atagacagga tacgcgatac
      361 tgccgaggct caccggcgga ccttcatagt tgaagtgatg ggtaggcact ccgggttcat
      421 agcgctggcc acagcgatcg gtggcggtgc ggatgcggtg cttgttcccg agatccctcc
      481 ggacatcccg cggattgtct cgatggttga aagacgtttt gccgccggca agcgtttttc
      541 gttgatcgtc gtcgcggagg gagtcgtaat cgatgatttc ccgggagcgg gaagtctggc
      601 gcacatcctg aaaaagcacg ggcttgactg ccgcgtttcc atccttggtc atcagcagcg
      661 aggcggcgca cctactgcgt tggatcgcga attcgcgtct ctgctcggaa aagaagctgt
      721 cgaggaactt ctgcgcgggg gaacggacag gatggcggga atagtcggga aacgcgtgga
      781 attctgtgac ttggagttct cgtggcgcga aaagaaaccg attgacatgg aggtgtaccg
      841 gtttatcagc gaagccgcga tttgacgtcg gccgttgagg ttactctcgc agccttcctc
      901 aagactgccc gtgccgcgaa tcgagaccct tcgcgcagtt tgcgatgggt gtgccggttc
      961 ccgtataata cttcacgacg ttggatacag ggttccttgc gttccgtttc ttctgcggac
     1021 cgttttcgcg gaaacaagcc ggggtaaaaa gagatcggga aatggcgcgg cgaataacga
     1081 ttgcggacat tgcgcgttat gcgggcgtat cccccgcgac ggtttccctg gttctgaacg
     1141 ggaaatacac gcaggggcag actgcaaggg gaaccgccga acgagtgtgg gaagccgcca
     1201 gagtcctggg ttaccgagga cgcataagga tgcgcccgga acccggcgcg cccgccatag
     1261 gcctgctgat tgccgataga gagtcctttt tcacaaccta ttatggcccg cggcttctca
     1321 agggaatgtg gcacgtcgca gatgagtaca acgcccatct gtttctgttc agcgatcgga
     1381 tggaagggca gcctgtaacc ttcgattacc tgagaaagga actggaatcc cgccgcgtga
     1441 atgaccttgt cgtgaatgtg aacaacaacc tgagcgagtc gttgcttgac acggtggagg
     1501 cgctcgcgat agggggcacg cggatcgtgg ctctgtggcg gaggccgaag tcgtggtttg
     1561 caccgtgcgt ggctcctgac gacgagaacg ccgcactgct catgctgtct caccttttcg
     1621 agctcggaca ccgttgtgtg gcgctcgttg cgactgaacc ggacgaacgc acgttgctca
     1681 tcaccgaaag gttcgccgcc gcggcgcggg agatgggtgt ggagttggct ttcggcgaag
     1741 ctctttttga aaacggcggc aagtggggcg ttctcgacga cgaccaggca gttgcccgat
     1801 tgctgggctg ttcttctcct ccgacggcca ttgctaccct gtacgatggt cgcgcggttg
     1861 gggtaacaaa ggcgttgggg tctctcggtt atcgtgttcc ggatgacatc tctctgatcg
     1921 cgtacggaaa tatgccggaa aaccgaccta ctccttacct gattacgatg gttcaatccc
     1981 cgatcgagca gatcggggag accgcagtac gccttttggt ggatccggaa tcctcgtatt
     2041 cggcatgttg cgccgggcct ccaatcagac tcccagtcgc actccgggtg ggcgccacca
     2101 cggggccggc aagcaaggcc ggtccgtact cccgttcacg cgttcttcgc tctttctgac
     2161 ccgggcaggt tccttccggg tttcccagtt tttctgccac gtatttcgtt cggagaacaa
     2221 gaccgtgaat tcgctcagag cagtatggat ggcgatcgcc gccgtgtttc tcgcaaatgc
     2281 ggcagattca cgagaatcag caacaatacg catcacggtt ggcccggatc ggtcggactg
     2341 gcctcatgcg ccggtggcaa ttcgcctgga tagcatcctg tcaagaaatg gatggaagtc
     2401 gccgctgggc aactcgttga ttcgagtgcg agagtcaggg aacgctcgtt tggtcccgag
     2461 cagggctggg tggaaccaca gcggtgagtg tcccgggctt cttctcttct cgaaaacggg
     2521 tccggggcga cgggaatacg tgattacggt gacatccgac gaagcgactc ccgcggggga
     2581 aaggattccc gtcgttggtt ttggcgagcc cctgacgcac ggcgtgacag ggattgtgtc
     2641 gggcatagac ggcagtttca acagcaatct cgctgttgca gactgggacg gagacggaga
     2701 tctggatttc ttcgtagcgc agggccaggg cgggggcgcc aactggacca tgcacggcct
     2761 tctgtattac gagaatgtgg gtacctctcg ctcaccgctt atggcgaaac ctcgaaggct
     2821 gttcgaaagc acctccttgc cgctggtagc ggat
//
LOCUS       DAOTNG010000370         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27393_length_2852_cov_7.189489, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000370 DAOTNG010000000
VERSION     DAOTNG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27393_length_2852_cov_7.189489"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2385)
                     /gene="secD"
                     /locus_tag="P5179_13325"
     CDS             complement(<1..2385)
                     /gene="secD"
                     /locus_tag="P5179_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRS96243.1"
                     /translation="MPRGLQIRIALLLVIIGWAVYQLYPTFVFWPLTVQQRQALQLTD
                     KAKFDDLQMRAIKRGLDLQGGVFLVLEVDPARTMTGGTLADAVEGARRVIEQRVNQYG
                     VSEANVQKIGERRIIVELPGLDNIEAAMRLVGRTALLKFNLLRPVSERDEVVKRIDAV
                     LPQLLSGVVIDTTASVDSSAAAAATPASRAATDSTPASQGELFGGARQPQTAQTGVAR
                     ADSARENPLKGLTSFSGLLNVGPGNDYYVSTVNKPKVEAVLSLIAEKNPGLIPSAGEF
                     LFGKEELINGQYVTPLYYVSSNAEMTGASVKDAQVSQGSNLDIEQAGRPVINFEVKED
                     SVRLFSALTRRNRGERMAIILDNVVYSAPTIQAHITDGRSIITGMADKEEARSLAIAI
                     RSGALPAEVQVQEQRYVGPSLGTDSIESSLRASMIAGIAIIAFMVLYYRLGGAMAVVS
                     QTLNLCLVMASLAALHGTLTLPGVAGLILTLGMAVDSNVLVFERMREESNSGKSPRAT
                     IESGFKNASSAIWDSNITTLISAIVLYQFGTGPVRGFALVLAIGIGGTVFAALFITRL
                     FYDLYAYFGRLRTISLGIPFLKNPHFQFLKWRKPWFIGSGIVLVVSVASILTVGFNWG
                     VDFTGGTRLAVRFVPPVKPGDVRTALRRVPVGNRVLDLSQSEIKTVDEATDLLITVKQ
                     ISNIEAAAVDSALRIRLSTAFPANTKGDWVLNAESVGPKVGSELKSSAIYAVLFSLIG
                     ILIYLSFRFEWIYAVTTTASLFHDVLLALGIFALIRHEVTLSVVAAVLTMVGYSL"
     gene            complement(2399..>2852)
                     /locus_tag="P5179_13330"
     CDS             complement(2399..>2852)
                     /locus_tag="P5179_13330"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRS96244.1"
                     /translation="TEGGLDVVGNREWVARAMERLHAAGIAVSLFIDPDPAQVRIAAE
                     LGAEAIELHTGEYANSRDAEQATQLHRLEAAAVLGCDLGLEVHAGHGLTYQNVSRVAS
                     IPQIVELNIGHSIIARALYVGLRQAVAEMRTLLAEARNEARLNSLSDK"
BASE COUNT          628 a          845 c          805 g          574 t
ORIGIN      
        1 cagggagtat cctaccatcg tcaggactgc cgctaccact gacagcgtga cttcgtgccg
       61 gatcagcgca aaaatgccaa gcgcgagaag aacgtcgtga aacagcgacg ctgtcgtcgt
      121 gactgcgtag atccactcga aacggaaaga caggtagatg aggatcccga tcagagagaa
      181 cagcaccgcg tagatggccg agctcttcag ctcggacccc accttcgggc caacggattc
      241 cgcgttcagc acccaatccc cttttgtgtt tgcgggaaag gcggtagaaa gtcgaatccg
      301 caacgcgctg tccactgcag ccgcttcgat gttggaaatc tgcttcactg ttatgagaag
      361 atccgttgcc tcgtcaacgg tttttatttc gctctgtgag agatccagca cgcggttgcc
      421 aacgggaacc cgtctgagag cagttctgac gtcgccgggt ttgacgggtg gcacgaagcg
      481 aacggcgaga cgagtacctc ccgtgaaatc cactccccag ttgaagccga cggtcagaat
      541 gctggccaca ctgacaacca gaacaatacc ggagccaatg aaccacggtt ttcgccattt
      601 caggaactgg aagtgaggat tcttcaggaa gggtattcct agtgaaatag tacggagcct
      661 cccgaaatac gcatacagat cgtagaacag gcgggtgatg aacagcgcgg cgaagacagt
      721 acccccgata ccgatcgcaa gaacgagcgc gaagccgcga acaggaccgg ttccgaactg
      781 gtacagcacg atcgcactga taagggtcgt gatgttggaa tcccagatgg cgctcgaagc
      841 atttttgaag ccggattcga ttgtcgcgcg cggtgatttc ccgctgttgc tctcttcgcg
      901 catgcgctca aaaaccagga cgttcgagtc gaccgccatt cccagcgtca agatcagtcc
      961 agcgacgccc ggcagagtca gcgttccatg caacgccgca aggctggcca tgaccaggca
     1021 gaggttcagt gtctggctga ccaccgccat cgccccgccc aggcgatagt acaacaccat
     1081 gaaggcaatg atcgcgatac ccgcgatcat cgacgctctt aggcttgatt cgatggaatc
     1141 cgttccaagc gaaggtccaa catagcgctg ttcctggacc tgaacctcgg cgggcaatgc
     1201 tcccgaccga atcgcaatag cgagactccg cgcctcctcc ttgtccgcca tgccggtgat
     1261 gatggatcgc ccgtcggtga tgtgcgcctg aatggttggc gctgaataca ccacattgtc
     1321 cagaatgatt gccatgcgtt cgccacgatt gcgccgggtg agcgctgaga acaggcgaac
     1381 cgaatcctct tttacctcga aattgatgac gggacgtccc gcctgttcaa tgtcgagatt
     1441 ggacccctgg gagacctgcg catccttcac gctggcgccg gtcatctcgg cgttggaaga
     1501 gacgtagtaa agcggggtta cgtactgccc gttgatcaat tcttccttgc cgaacagaaa
     1561 ctcccccgcc gagggaataa ggccggggtt cttttcggca atcaatgaca atactgcttc
     1621 gactttcggc ttgttcaccg tggacacgta gtagtcgttg cccggcccga cattcagcaa
     1681 gccgctgaac gatgtgagtc ctttgagcgg attctcgcgt gcagagtcgg ctcgtgccac
     1741 gcctgtctgg gcggtctgtg gctgccgggc accgccgaac aactcgcctt gcgatgcggg
     1801 agttgaatcc gtagccgcgc gagatgccgg tgttgcggca gcggcggcag aggaatcaac
     1861 ggaagctgtg gtgtcgatta ctaccccgga aagcagttgg ggcaaaactg cgtcgatacg
     1921 tttcacaacc tcgtcgcgtt ctgatacggg cctcaggagg ttgaacttga gaagcgcggt
     1981 acgtcccaca aggcgcatcg cggcttcaat gttgtccaga ccgggcaatt cgacgatgat
     2041 ccgccgctca ccgattttct gaacgttcgc ttcagaaacg ccgtattgat ttacgcgctg
     2101 ttcgatgacg cgcctcgctc cttccacggc gtccgcaagc gtccctccgg tcatcgtgcg
     2161 tgccgggtcc acctcgagta cgagaaaaac acctccctgc aggtcaaggc cccgtttgat
     2221 cgcgcgcatc tgcaaatcat cgaacttcgc cttgtcggtc aactgcagag cctggcgctg
     2281 ttgcaccgtc agcggccaga acacgaacgt cgggtaaagc tggtagacag cccagccaat
     2341 tatcaccagc aacagggcaa ttctgatctg caacccacgt ggcatgcgtg gcaactcctt
     2401 acttgtcaga caaggaattc aggcgcgcct cattccgggc ctcggcaagc agcgtgcgca
     2461 tttcggcgac ggcctgcctg agacctacgt acaacgcgcg agcgataatg gaatgaccga
     2521 tattgagttc gacgatctgc ggaattgacg caacacgtga cacgttctgg tatgtcaggc
     2581 cgtggcccgc gtggacttcc agaccaagat cacatcccag aacggcggca gcttccagcc
     2641 ggtggagttg tgtggcctgt tccgcatccc gggagttggc gtattcgcct gtatgaagct
     2701 caatcgcttc cgcacccagt tccgcagcaa ttcgaacttg cgcagggtcc ggatcgataa
     2761 acaatgacac cgcaatacct gccgcatgga gccgttccat ggcgcgcgca acccattcgc
     2821 ggttgccgac gacgtccagc ccaccttcag tc
//
LOCUS       DAOTNG010000371         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27499_length_2845_cov_6.568100, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000371 DAOTNG010000000
VERSION     DAOTNG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27499_length_2845_cov_6.568100"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5179_13335"
     CDS             complement(<1..585)
                     /locus_tag="P5179_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRS96245.1"
                     /translation="MQPENNSGACFSADRYFLKDAVRQAVDFHQTAQAQGLPAPPLQK
                     PASPDARHVALPDGSTALLRLCAMSLGEAISLRESVRLYSSDALSLEELSALLWATQG
                     VRKVLRPDCALRNVPSAGARHSFETYIAAARVRDLPAGLYRYLPFDKELIELTLDPEI
                     GQKARNACLGQEFVSRAAATFFWTTIPARMEWRYD"
     gene            complement(640..2571)
                     /locus_tag="P5179_13340"
     CDS             complement(640..2571)
                     /locus_tag="P5179_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96246.1"
                     /translation="MPFPVIRWPRVLIWGLSVVVLMNVWMAYCEYVVRASRLNGSHFP
                     VALVAAMAALVLAVWPLLKAIGGSNWGISTPERHLILAMGFVGAAIPANGLTGFMMGI
                     IATPYYYATPENNWASFHPFIPGWIVPPDKHHAMRWFFNGLPPGEPIPWRVWWVPLIW
                     WFSLAAAIMGVCLALSIILRKQWVEYERLSYPLVEASKCLLAGKDDPRGRKPVWQSRL
                     FWVGTTIAAFILVYNTFAWFDPRWPMIDIAGRGIVLFRGVPDLKTRINFLTLGLSYFA
                     PQQVLGSLIVGCLVTTAEAGAFARVGFSLDPAGDSWTNDNVAIGWQSFGAMMVFVLWG
                     LWQARRHFVAVWRSAWTGTDDGTEMLSYRVALIVLTLSLFYMIMWLHEAGMDVSFAIT
                     FVFFTVIGYLALGRMVAEAGYVYQRVPISPQSATVYTLGVESFHPATMAGFAFSYALI
                     ANGRGLFMPAVVQATRLADSSGKAARKALGAIALAFVVGFAVSVVYTLYLGYTYGAFN
                     FRVYPFSGGNYEAFTHTIRKIKNPFGPDVARLSMAGAGSAVMAGLIFLRYRFPGFAFH
                     PIGMVFPFTYLTRFAVFSISITWLVKFILLRLGGVELYRKTQPLFLGLAVGYALGVAY
                     SFVVDWIYFPGAGHVIHSW"
BASE COUNT          664 a          852 c          822 g          507 t
ORIGIN      
        1 gtcgtaacgc cactccatgc gggcgggaat cgttgtccag aaaaaagtcg cggcggctct
       61 cgaaacgaac tcttgcccga ggcacgcatt tctggccttc tgaccgattt ccgggtccaa
      121 tgtgagttcg ataagttctt tatcaaaagg aagataccgg tacaaccccg ccggcagatc
      181 ccgaactcgt gccgccgcga tgtacgtttc gaatgagtgc cgcgcgccgg ccgacggcac
      241 gttccgcagc gcgcagtccg gacgcaggac ctttcgcaca ccttgagttg cccacaatag
      301 agccgacagt tcctccagcg acagtgcatc ggaggaatac aaccgcacgc tttcccgcag
      361 agatatcgcc tcgccgagac tcatcgcgca cagccggaga agtgctgtgg agccgtcagg
      421 taatgcgaca tgtctggcat ccgggctggc cggcttctgc agagggggtg cagggaggcc
      481 ctgtgcttgg gcggtctgat ggaaatcgac agcctgacgg acggcatctt tgaggaaata
      541 cctgtcagca gaaaagcatg cgccgctatt gttctcgggc tgcatcggct atccctttcg
      601 tctagtcacc caccgctgcc acgtgagtcg tggtttctct taccaggaat ggataacgtg
      661 tccggcaccc gggaagtaga tccagtccac cacaaaactg tacgcgactc cgagcgcgta
      721 ccccaccgcc agtccgagaa ataacggctg cgtcttccgg tacagctcca cgccgccaag
      781 gcgcagcaga atgaatttga cgagccacgt gatgcttatc gaaaacaccg cgaagcgggt
      841 gaggtaggtg aatgggaaga ccatgccgat cgggtggaac gcgaaacccg ggaaccggta
      901 ccgcaggaag atcaatcccg ccatcacagc gctgcctgct ccggccatac tcagacgcgc
      961 aacgtcgggg ccgaagggat tcttgatctt gcggattgtg tgggtgaatg cctcgtagtt
     1021 gccgccggag aacggatata ccctgaaatt gaacgcaccg taggtgtacc cgaggtagag
     1081 cgtgtatacc acggaaacag cgaaccctac aacgaacgcc agggcaattg ctcccagtgc
     1141 ctttcgagcg gctttgcccg aagaatcagc aagacgtgtg gcctgtacga ctgccggcat
     1201 gaacaaccct cgtccgttcg caataagcgc atacgagaac gcaaaacccg ccatggttgc
     1261 cgggtgaaat gattccaccc ccagcgtgta tactgttgcg gactggggac ttatgggcac
     1321 tcgttgatac acatagcccg cttcggcgac cattcgtccg agcgcaaggt aaccgatgac
     1381 cgtgaaaaag acgaatgtga tggcgaagct cacgtccatg ccggcttcgt gaagccacat
     1441 gatcatgtag aaaagggaga gcgtgagcac gataagggca acccggtaac tcaacatctc
     1501 cgttccatcg tcggtgcccg tccacgcgga gcgccaaacc gccacaaaat gccttcgcgc
     1561 ctgccagagg ccccaaagga cgaacaccat catcgccccg aaactctgcc atccgatcgc
     1621 gacgttatcg ttggtccagc tgtctccggc ggggtccagg ctgaaaccca cgcgggcaaa
     1681 cgcgcctgct tccgcggttg tcacgaggca accgacgatc aggctgccga gcacctgttg
     1741 gggtgcgaag taggaaaggc ccagtgtgag gaaattgata cgtgttttga ggtccggtac
     1801 cccgcgaaac aagacaatgc cgcggccggc gatatcaatc atgggccagc gcgggtcaaa
     1861 ccaggcaaag gtgttgtaca ccagaatgaa cgccgcaatc gtcgtcccga cccagaacaa
     1921 ccgcgattgc cacacaggtt tgcgtccacg gggatcatcc ttccccgcaa gaaggcactt
     1981 actcgcttcg acaagcgggt acgaaagccg ttcgtactca acccactgtt tgcgaagaat
     2041 gatggacaac gcgaggcata cccccatgat ggcggccgcg agcgaaaacc accagatcag
     2101 cggaacccac cacacacgcc acgggatcgg ttctccgggg ggaaggccgt tgaaaaacca
     2161 tctcatcgcg tggtgtttgt caggagggac gatccaaccc gggatgaagg gatgaaagct
     2221 tgcccagttg ttctcaggcg tagcatagta atacggagtg gcgatgatac ccatcatgaa
     2281 cccggtaagc ccgttggccg gaatcgccgc accgacaaag cccatggcaa gaatcaggtg
     2341 tcgttcagga gtgcttatgc cccagttgct tccgccgatc gctttcaaca gcggccatac
     2401 ggccagcacc agggcggcca tcgcagcaac cagcgccact ggaaaatggc tgccgttcag
     2461 ccggctcgcg cgaacaacgt attcgcaata ggccatccag acattcatca acaccacgac
     2521 cgaaagcccc cagatcagaa ctcgcggcca gcgaatcacc gggaaaggca cggaagacgc
     2581 gtcgtgggtc acgcctggtg gcatggtcgg tggagaaggg gttccgttcg cggtatttct
     2641 cccgtatgcg agggcaagag tggtttgacg gatttgacgc ggatgtgccg ggaaatccaa
     2701 gaataaccgg ttgcgcacgg ttccggcaag gcaggcgccg gtcatttcgc cgtgccgacc
     2761 acacgtgcgc catgcggtat tcattcagtc gccccttgcc cccggcgcac gttccgtagc
     2821 acctggtaca atcagcattc cagtg
//
LOCUS       DAOTNG010000372         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27820_length_2821_cov_6.063630, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000372 DAOTNG010000000
VERSION     DAOTNG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27820_length_2821_cov_6.063630"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="P5179_13345"
     CDS             complement(<1..837)
                     /locus_tag="P5179_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS96247.1"
                     /translation="MAQIRVLVCDDEPDARFLLRSRLVSLGYDVLEASDGDECVKIAA
                     SDPVDIIILDVSMPRVDGFSACSELRRNPRTRHIPIVFLTALRTSRDDRLKGLRLGAD
                     EYLVKDVDPEELAARLEAVLRRTVGAVETNPLTHLPGNAVVTEEIESRIRKREPFAVA
                     WADLDNFSAFNDRYGFARGDKLIVETATILLESLKLFGSEEDFLGHLGGDDFVVVSTV
                     ERSAMMAEHAVKLGRERLPLLYDLDDRSRGYVRTLDRNGKQLQYPITNISVAIVQSRG
                     QRF"
     gene            1094..1591
                     /locus_tag="P5179_13350"
     CDS             1094..1591
                     /locus_tag="P5179_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96248.1"
                     /translation="MAVRSASRSSAVTARSVIGGSLLVVLVSIVTPYSEYRLHSVELF
                     QGQLPLGALATLVVIVLPLQWLLARFFPAWRLRESEILFMFSMGFAGLMVYHIGMMGL
                     FLSMVSSPEYFASPENQYARYLLPYLPAWAIVPNSNSAMTWFYTGLPAGAAIPWRVWV
                     GPLFW"
     gene            1811..>2821
                     /locus_tag="P5179_13355"
     CDS             1811..>2821
                     /locus_tag="P5179_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96249.1"
                     /translation="MSYFRPIWPRIDFSQMAPWVLNFGPGFGVVDLVPDPFVIGISYL
                     VDVKILFSVWFFFLLGRVQQAVQVKTGYVSGQTDLWTTSDNLSGWQSLGGLFFVVFWF
                     LWMSRAHFKRVLQHVLHPETSQEDDSNEMLSYRMSLTLLFACSVYMVLWLIHLGLDWY
                     VALVFFVALVVLVLGITRIVAETGMPLVGSPVTAQGITTRLFGDAALGPSNIAGLAVT
                     YAAFRMVEGYPMPVTMHAARLGDSAGASRRTLFGAVLFGSVAAMLVMSVVTIALAYNG
                     GAFNFGAHHAFHQMYEAYDHMTGRIRSPYPWDWRLFAHFGGGALFVGLLTFLRYRFGW
                     DSL"
BASE COUNT          483 a          831 c          826 g          681 t
ORIGIN      
        1 gaatctctgc ccccgtgatt gaacgatcgc gaccgagata ttggtgattg gatactggag
       61 ttgcttcccg ttacggtcca gggttcgcac ataccctctg gagcgatcat ccagatcata
      121 gagaagcgga agcctctcgc ggccgagttt cactgcgtgc tctgccatca ttgcggagcg
      181 ctctaccgtc gataccacca cgaagtcgtc gccgccgagg tgcccgagaa aatcctcttc
      241 gctcccgaac agcttcaggc tttccaggag gattgtagcc gtttccacga tcaatttgtc
      301 gccacgcgcg aacccgtacc gatcattgaa cgcgctgaag ttgtcgagat ccgcccacgc
      361 aaccgcaaat ggctcccgtt tccggatacg cgattcaatt tcctcggtga ccaccgcgtt
      421 gccgggcaga tgggtaagcg gattcgtctc gaccgcgcca acggtacgcc gcaataccgc
      481 ttccaaacga gccgccagtt cttcagggtc cacgtccttc acgaggtact cgtcggcgcc
      541 caggcggagg cctttcaagc ggtcgtcgcg gctggttcgc agcgcggtga gaaacacgat
      601 cggaatatgt cgagttctcg gattgcgacg cagttcagag caggctgaaa acccgtccac
      661 gcgcggcatg gagacgtcaa gtatgataat gtcgaccgga tcacttgccg cgattttgac
      721 acactcgtcg ccgtccgacg cttcgagaac atcgtacccc agagacacaa gccgcgaacg
      781 caacaggaag cgtgcatctg gttcatcgtc gcatacgaga actctgatct gtgccatgaa
      841 gtctcccggg tgggcgtggt gatgtgtggc gactaatgac cacacaggcg caatccatca
      901 tctggaggcg cggaaaaatg tgatggattt tacagaatat agcgtcttgc cgcgcggggt
      961 caacacaccg taaggccggg ttgcccctgt tttggccctt tctgatcttt ttcctcgcgt
     1021 gcttcgggca gtacggtgcc atcgatccgc atgggcgatg gccccgcggc accaaccctc
     1081 ggcgggttga tccatggccg ttcgttccgc gtctcgctcc tccgcggtta ccgcccgatc
     1141 cgtgatcggc gggtccctgc ttgttgttct cgtcagcatc gtcacaccgt attccgaata
     1201 ccgccttcat tccgtcgagc tgttccaggg acagcttccg ctgggcgcgc tggcaacgct
     1261 tgtggtgatc gttctcccgc ttcaatggct tctcgcgcgt ttcttccccg cgtggcgtct
     1321 tcgcgaatcc gagatcctgt tcatgttctc catgggtttc gcgggattga tggtgtatca
     1381 catcggcatg atgggcctgt tcctcagcat ggtctcatcc cccgagtact tcgcgagtcc
     1441 ggaaaaccag tacgcgagat accttctgcc gtacctcccg gcgtgggcga ttgttccgaa
     1501 ttccaactcc gccatgacct ggttttacac cgggctcccg gccggggcgg ccatcccgtg
     1561 gagagtgtgg gtcggcccgt tgttctggta gtggtcgttt ttcttcgcat ttctcgtgct
     1621 ctgcggatcg ttaaccgccg ttctgcggaa acaatggttc gaccacgaga agattcgctt
     1681 cccgcaggcg gaagttaccc ttgcgctcgt ggaggggtca gggggcgcat cgggccgaaa
     1741 caccgtatcc ggcagccccc cattctgggt cggttttgca ttgtcggcgg gcatcctcgt
     1801 atggaacagc gtgagttact tccgccctat ctggccgcga atagattttt cgcagatggc
     1861 gccgtgggtg ctgaatttcg ggccaggatt cggcgtagta gacctcgttc cggatccctt
     1921 tgtgatagga atttcttacc tcgttgatgt caagattctt ttcagcgtat ggttcttctt
     1981 tcttctcgga cgtgtgcaac aggcggtcca ggtgaaaacc gggtatgtca gcgggcagac
     2041 tgacctgtgg accacgagcg ataatctgag cgggtggcag agcctgggtg ggcttttctt
     2101 tgtggttttc tggttcctgt ggatgtcgcg ggcgcatttc aaacgcgttc tgcagcacgt
     2161 gttgcacccg gagacgagcc aggaggacga ttcaaatgaa atgctctcgt atcggatgtc
     2221 attgaccctg ctcttcgcgt gcagcgtcta catggtgctg tggctcatcc atctcggcct
     2281 ggactggtac gtcgccctcg tctttttcgt tgcacttgtg gtccttgtcc tcggaatcac
     2341 ccggattgtc gcggaaaccg gaatgccgct ggtgggaagc ccggtgacag ctcaggggat
     2401 cacgaccagg cttttcgggg atgcagccct cggcccatcc aacattgccg gcctcgcggt
     2461 tacttacgcg gccttccgta tggtggaagg gtacccgatg cctgtgacaa tgcatgccgc
     2521 tcgtctcgga gacagcgccg gcgcctcacg caggacgttg tttggcgcgg tgctttttgg
     2581 ctccgtcgcc gcgatgctgg taatgagcgt ggttacaatc gccctggcgt acaacggcgg
     2641 cgcgttcaat ttcggcgcgc atcacgcgtt ccaccagatg tacgaagcat acgaccacat
     2701 gacgggcagg attcgctcgc cctacccgtg ggattggcgt ctgttcgcgc attttggcgg
     2761 cggagcattg ttcgtcggtc tgctcacgtt tctgcggtat cgcttcgggt gggattccct
     2821 c
//
LOCUS       DAOTNG010000373         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_27842_length_2820_cov_3.895841, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000373 DAOTNG010000000
VERSION     DAOTNG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27842_length_2820_cov_3.895841"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="P5179_13360"
     CDS             complement(<1..1229)
                     /locus_tag="P5179_13360"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96250.1"
                     /translation="MPDFSVIAKNFADALIAYGTDHYGTRDTPLFCHFIDLETLTVPA
                     QKTAAEWRDLMADWEEDKGYLMWGKDRSNLTWAHQSNLLWDTETVRLLIKLSESTGDR
                     RYAQAAWSCMAYFLEHCVSRETGLFAWGEHVAYNVVSDRIEGERHELQHADPLWEELW
                     QISPTKVQREIEAIYRFHVVDKAAMIYDRHARFWDGLPERDQATILGYAGVFAKAWAF
                     LWTKTRKPEYLRWARNQLLAFQSKSNGAGLYPDNWTDSDKRQLPRNYPARPGLARDMF
                     DIYVMTGMREWLDDGCRYLEACVGLRHEESRTMETKGDPVFGSDVAAGIEVLISAFNL
                     TRNQQWRDLAEEYGRTLAAEPIPTPLMASRLAERIQAFCALHAATGDPRWLSCATRDA
                     EYAITTFVHSSGLIKGTA"
     gene            1210..2565
                     /locus_tag="P5179_13365"
     CDS             1210..2565
                     /locus_tag="P5179_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96251.1"
                     /translation="MTEKSGIPHLSRWRPVSFWPLCSDFQYQERKENIGETNSRPSAR
                     AGFSLGAAFRLLHTLLSVPATWKSFSHSAVFWLREPGFPLEEFMYRHIPLRHPRPTAQ
                     RFIDGLMGRFKETKPRMVEYLVDGSVMKPIVTSLLGREWVDTPTDVESRRKALDIAIE
                     FWYRMGYDFIRIERGLVFPKHSLRADDVSGVPGNQRTWADEHQGMIRTWEDFEKYPWP
                     RLEDCDFFDLEYVSRNLPDGMGLIASHGGGPFEQLSQIFSLEGLALALYDDPDLVKAV
                     NDKIGNLMTGFYRHLVQLDNLVAVFPGDDMGFRTGTMIAPAHLRAYTLPVHTRVAEMA
                     HERGLPYFLHSCGNVLPIVEDVINDVKLDGKHSFEDAIIPIQDFQREYGEKLAVLGGV
                     DIDLLAAGSVQDVRKHVRFLMETCGARGRFALGSGSSIPNYVPVANYLAMLDEAIDFS
                     S"
     gene            2573..>2820
                     /locus_tag="P5179_13370"
     CDS             2573..>2820
                     /locus_tag="P5179_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96252.1"
                     /translation="MARAKALLDNVCVALNREGVAAERHIPLAAYTTFRIGGVADILA
                     TATTEAQIAAAVRVARKFDTGLRCIGGGSNLLVSDDGV"
BASE COUNT          540 a          791 c          854 g          635 t
ORIGIN      
        1 gccgttcctt tgatgaggcc actggaatga acaaacgtcg tgatcgcgta ttccgcgtcg
       61 cgtgtggcgc aagagagcca tctcgggtca ccggtggcag catggagggc gcagaacgcc
      121 tgaatacgtt cagccagccg tgacgccatg agtggagtcg gaattggctc agcagcgagc
      181 gtgcgaccat attcctcggc gagatcgcgc cattgctggt tgcgcgtcag gttgaacgcg
      241 gaaatcagaa cctctattcc ggctgcgaca tcgcttccga acactggatc gccttttgtt
      301 tccatcgtgc ggctttcctc gtgtcgaagc ccgacgcatg cctcgaggta gcggcacccg
      361 tcgtccaacc attctcgcat accggtcatc acgtagatgt caaacatgtc tctggcgagg
      421 ccgggacggg cgggatagtt gcgcgggagt tgccttttgt ccgagtccgt ccagttgtca
      481 gggtagagac ctgcgccgtt tgatttggac tggaacgcca gaagctgatt tcgcgcccaa
      541 cgcaggtact ctggtttccg cgtttttgtc cacaggaacg cccaggcttt ggcgaataca
      601 ccggcgtacc cgaggattgt cgcctggtcg cgctcgggca ggccgtccca gaagcgcgcg
      661 tgtcggtcgt aaatcattgc tgccttgtcc acgacgtgaa aacggtaaat cgcctcgatt
      721 tcgcgctgga ctttcgtcgg tgagatttgc cagagttcct cccagagggg gtcggcgtgc
      781 tgcaattcgt gccgttcccc ctcaattcga tccgacacga cgttgtatgc aacgtgttcg
      841 ccccaggcaa aaagccccgt ttctctgctc acacaatgtt ccaggaaata cgccatgcat
      901 gaccaggcag cctgggcgta ccgccgatcg cctgtgctct cgctgagttt gataaggagc
      961 cgaacggttt cggtatccca gagaaggttt gactgatgcg cccacgtgag gttgctccgg
     1021 tcttttcccc acatgaggta tcctttgtcc tcttcccaat cggccatcag atcgcgccac
     1081 tcggcggcag ttttctgggc gggtaccgtc aacgtctcca gatcgatgaa gtggcagaac
     1141 agaggggtgt cacgggtgcc gtagtgatcg gttccgtacg cgatcaacgc atccgcgaaa
     1201 ttctttgcga tgacagagaa atcgggcatc ccgcaccttt cccggtggcg tccggtgagt
     1261 ttctggccgc tctgttcgga tttccagtac caagaacgca aagagaacat tggcgaaaca
     1321 aactcccgcc cgagcgcacg cgccggcttc tcccttggtg ccgcgttccg cctgttgcat
     1381 actttgttgt cggttcccgc aacctggaag tcattctcgc actctgcagt tttctggctc
     1441 cgcgaaccgg gcttcccctt ggaggaattc atgtaccgcc atatcccact ccggcatccg
     1501 cgaccgaccg cgcagcgctt cattgacgga ttgatgggcc gtttcaagga aacaaaaccg
     1561 cggatggtgg agtaccttgt ggacggttcc gtcatgaagc ccattgtcac gtcgcttctg
     1621 ggcagggagt gggttgacac gccaaccgat gtggagagtc gcaggaaggc gctggatatc
     1681 gcgattgagt tctggtaccg catggggtat gatttcatcc gcattgaaag aggactggtg
     1741 tttccgaagc attccctgcg cgccgacgac gtgtccgggg tgccaggaaa ccagcgtacc
     1801 tgggcggatg agcaccaggg gatgatccgc acgtgggaag attttgagaa atacccctgg
     1861 ccgaggctgg aagactgtga ctttttcgac ctggagtatg tgagcaggaa tctccccgac
     1921 ggcatggggc tgatcgcgag ccatggcggt ggaccgttcg agcagttgtc gcagattttt
     1981 tcgctggaag ggcttgcgct ggcgctctac gatgaccccg atctggtgaa ggcagtgaat
     2041 gacaagatcg gtaatctcat gactggcttc taccggcacc tcgtccagct cgacaacctc
     2101 gtcgccgtgt ttcccggaga cgatatggga ttccgcacag gcacgatgat cgcacctgct
     2161 catctccgcg cgtacactct tccagtgcat acgcgtgtcg ccgaaatggc gcacgaacgg
     2221 ggtctcccgt attttctgca ctcatgtggg aatgtccttc cgatagtcga ggacgtgatc
     2281 aacgacgtga agctcgatgg aaagcattca ttcgaggacg cgatcattcc gattcaggat
     2341 ttccagcgcg aatacgggga gaagcttgca gttctgggtg gggtggacat tgacctcctc
     2401 gcggccggct cggttcagga tgtgaggaaa cacgtgaggt ttttgatgga aacctgtggt
     2461 gcgcgaggac gtttcgcact cgggtccggg agctctattc ccaattacgt tccggtagcg
     2521 aactacctcg cgatgctgga cgaagcgata gatttctctt cgtaggtatt tcatggcgcg
     2581 cgcgaaggct cttctggaca acgtttgtgt cgcactcaac cgcgagggtg tcgcggcgga
     2641 gcgacacatc ccgctggcgg cgtacaccac gttcaggatc ggtggtgtcg cggacattct
     2701 ggcgacggcg accaccgaag cccagattgc cgcggccgtt cgcgtcgcgc gaaagttcga
     2761 tacagggctc cggtgcatag gaggggggag taacctcctg gtttccgatg acggtgtgga
//
LOCUS       DAOTNG010000374         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_28466_length_2770_cov_4.740331, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000374 DAOTNG010000000
VERSION     DAOTNG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28466_length_2770_cov_4.740331"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(441..2615)
                     /locus_tag="P5179_13375"
     CDS             complement(441..2615)
                     /locus_tag="P5179_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS96253.1"
                     /translation="MNERIARIRTRALRSYGVHDPERDALRKESFAQTEGEPTVIREA
                     KAWAHYRRNQTLVILEDELIVGGHIGIAYDPEKNSVPEIFGRQGFKCFWNVSDEVQKY
                     FNEGMLSGAGNHTTMDYATIFEIGFEGLIQRIRNRAARLQQDEHDLLEKRDFLAALEI
                     MAEGYIALCDRYADYAAELAAETIDETRRAELETISRNCRRVPAKPPTTFWEACQAAW
                     FCFFFLADAPGRVDQYLYPFYRSDLEKGLITPDFARELLSCLWVKYFELAGARGAVSA
                     FHHLTLGGVHADGSDASNDVTMLCLDVTEDLRLNRPQIGVRWNRNMPREVLRRAVRVL
                     RTHCGNPDFCNDEQIVPALVRTGIRIEDARDFSLSGCHEVIVTGKAQMGSVEGFINLP
                     KVLRLMLGLEPNLGPGANLAAITDEETLWAAFEEAMDAVAERAHQASLARDIAAAKEP
                     GGNLHASLVVNDCIENVRGYTQGGARYNHCNWDVVGIANVTDSIATIRSLVFENGELT
                     LQELADIVRDNWQGHEALRQRVLNRCPHFGNDDEAADRIAVRIIKTMSDIMKRRKPFR
                     GGEYILGTLAGGENMHVEFGRMTGATPDGRLSGNTFADSIGPVHGRDRRGVTAMLNSV
                     AKLPHALLPTAVSLNIKLNPELIADERGVNNVAALIEGHFLAGGQQCQFNMVTREMLE
                     DARENPEKYRGLMVRVAGYSAPFTSLWADLQEEIIARTEHTC"
BASE COUNT          464 a          908 c          845 g          553 t
ORIGIN      
        1 cggagagcag tgcgcgtgcg gtagtcccgg gcagtctgct ggacagttgg cgttggacag
       61 ggaggcgcgg tcacgatgcc cccggagttg tgccgggcgt ctcgatacga tgagcggtca
      121 ccactgtccg cccatcactc gacgaccggc atttcgagcg atgcggccaa tgtgtttcag
      181 ttttgcgtga gtgggcgcca ccgcgcgccg accgaaggcg gtcgtcgagt ggcgcggcgg
      241 tcgtcaagcc gctgcgttgt atcgagacga gcgacgttca cccggttatc cgggcacaat
      301 ggccccgtgc tggccgagca gggatcgaat ttcctccagc ggcacctctc acacatcgca
      361 ccggatctgc gccgcaagcg ccgcagccgc tccggcgacc tgcccggtcg ccgcccgttg
      421 tatcgagacg accgccctcc tcagcaggtg tgttccgtcc gggcgatgat ctcctcctgc
      481 agatccgccc agagcgaggt gaagggcgcg ctgtatcccg caacgcgcac catcaacccg
      541 cggtatttct cgggattctc ccgcgcgtcc tcaagcattt cgcgtgttac catgttgaac
      601 tggcactgct gtccgccggc gagaaagtgc ccttcgatga gcgccgccac gttgttcacg
      661 ccgcgttcgt ccgcgatcag ctccggattc agcttgatgt tgagcgatac ggcagtgggc
      721 aggagcgcat gcggcaactt cgccaccgaa ttcagcatcg ccgttacccc gcggcggtcc
      781 ctgccgtgaa ccgggccgat gctgtcggcg aacgtattgc cgctcaggcg gccgtcaggg
      841 gtcgcacctg tcatacggcc gaattccacg tgcatgttct cgccgcccgc cagagtgccc
      901 aggatgtact cgccgccccg aaacggtttt cgtcgcttca tgatgtccga catcgtcttt
      961 atgatgcgca ccgcgatacg atccgctgcc tcgtcgtcgt tgccgaaatg gggacaccgg
     1021 ttcagcacgc gctgccggag cgcttcgtgc ccctgccagt tatcccggac gatgtcggca
     1081 agctcctgga gggtgagttc gccgttttca aacaccagcg accggattgt cgcgatggag
     1141 tcggtcacgt ttgcgatacc gaccacatcc cagttgcagt ggttataccg tgcgccgccc
     1201 tgagtgtacc cacggacgtt ttcgatgcaa tcgttcacga ccagagacgc gtgcagattc
     1261 ccgcccggtt ctttggcggc agcgatgtcg cgcgcaagag acgcctggtg cgctcgctct
     1321 gccacggcat ccattgcttc ctcgaaggcc gcccagagcg tttcctcatc cgtgatggcg
     1381 gccagattgg cgccggggcc gaggttcggt tcaagcccca gcatcagtct cagaaccttc
     1441 ggcaggttga tgaacccctc gacactgccc atctgcgcct tgccggtaac tatcacctca
     1501 tggcagccgg aaagcgaaaa atcgcgcgcg tcctcgattc ggattcccgt cctgacaagc
     1561 gccggcacga tctgttcgtc gttgcagaaa tccggattcc cgcaatgggt gcgcaacaca
     1621 cgcaccgccc ggcggagcac ttcccggggc atgtttctgt tccaccgtac gcctatctgc
     1681 gggcggttga gcctcaggtc ctcagtgacg tcgaggcaca gcatcgtcac atcgttgctt
     1741 gcgtcactgc catcggcgtg cactccgccg agcgtgaggt ggtgaaacgc gctcaccgcg
     1801 ccccgtgccc ccgcaagttc gaagtacttc acccacaggc acgaaagcag ctcccgcgcg
     1861 aaatccggcg taatgaggcc cttctcgagg tcggaacggt aaaacgggta gaggtactgg
     1921 tccacgcgac ccggcgcatc ggcgaggaag aagaaacaga accacgccgc ctgacacgct
     1981 tcccagaatg tcgttggcgg ctttgcggga accctgcggc agttgcgcga gatggtctcc
     2041 agttcggccc gccgcgtttc gtctatcgtt tcggccgcca gttctgccgc gtagtcggcg
     2101 taccggtcgc acagagcgat gtatccctcg gccatgattt ccagcgccgc caggaaatcc
     2161 cgtttctcga ggagatcgtg ctcgtcctgc tgcaggcggg cagcgcggtt ccgaatccgc
     2221 tgaatgagcc cctcgaagcc gatctcgaaa atggtggcgt agtccatggt ggtgtggttg
     2281 cccgcgccac tcaacatgcc ctcgttgaag tacttctgca cttcgtccga tacgttccag
     2341 aagcacttga acccctgcct cccgaatatc tccgggacgc tattcttctc cgggtcatac
     2401 gcgatgccga tgtgcccccc gacgatcagt tcatcctcga gtattacgag cgtctggttc
     2461 cgccggtagt gggcccacgc ctttgcctcg cggatgaccg tcggctcccc ttcggtctgc
     2521 gcgaaggact ccttccggag cgcatcgcgt tcggggtcgt gcacgccgta gctgcgaagg
     2581 gcccgggtgc ggattcgggc aatccgttcg ttcatcgagc atctcctgag gggttcgcgt
     2641 ggcgagttat cccacgccgt caaccggaca tcaacctctt tcccggggaa tagaagtact
     2701 cacctgaggg agtcgtggag caacggggtg gcatcaggga tgccgcgtgc gcggtacggg
     2761 ctggccgggg
//
LOCUS       DAOTNG010000375         2769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_28483_length_2769_cov_5.060059, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000375 DAOTNG010000000
VERSION     DAOTNG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28483_length_2769_cov_5.060059"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1425)
                     /locus_tag="P5179_13380"
     CDS             complement(151..1425)
                     /locus_tag="P5179_13380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS96254.1"
                     /translation="MADTLAIRGGTPAVPPGLKFRMWPEITKEDEELVLASLHQDSHA
                     FGPHADMLQKEFAAWNGNTFCMATNSGTAALHMCVAACGIGPGDEVITTALSWTSSAT
                     CIIHHNAIPIFVDVDWRSMHMDPSKIEAAITPYTRAILVVHYWGVACDMDAIMSIARR
                     HNLAVIEDACQAHGATFKGRKVGTIGDVAAFSLNQNKNLCGGEGGLFVTNDPGLFERG
                     KMVTSFGDMRPPDSGRDYHAYGLGWMYRTSDLPAAFALSQLRKLDRTNKWARDNWDML
                     DERLAGTPNLVRSFTSEDRPTNGYAYVLRVEPAYAERRGVPLRKITEGIVAALKAEGV
                     NFSIANWLLPAHAVFQAKNAYGKGEPWTSAFARKDVSYDLSQYPVSQQCIDTCLWGVF
                     NHRPPNGPEQINALANAIRKVFENLDDVPVEP"
     gene            1607..>2769
                     /locus_tag="P5179_13385"
     CDS             1607..>2769
                     /locus_tag="P5179_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96255.1"
                     /translation="MTPHLIWRKETGYQLNGIVSGMVDGQVRLYLSGIRTVSGRRESA
                     IFCLSSEGETVWQTQVADAAEDRLACVFPNLIDDPFLGLCLFYSFGSSDNARPRPGYA
                     RLVRAESGDVVWELRSRSHHAGNGSCIAADLDGDGRPEIAWWDSAHVFCSDLRTGDLR
                     WEYSDRVQICHGRPALAHLRGIPAIIVGTEYSNPDGTSSVLALDGSGRVLWRKDGFPE
                     DLGSTPVILADVDHDGRDEFVIAGLDLVHRDNRETSSLWCFRDDGELAYRVPCGCGGL
                     AVARVRGELLAAGITNTRDGGNNGAQEVRLFSLATGVLRHSDPLPRAYLDAQNPVAAD
                     LDGDGLPDFLLTTGNPSGYGNRPAIRPYADVYVVRADGATLWTETFPDYIHQP"
BASE COUNT          518 a          845 c          832 g          574 t
ORIGIN      
        1 atccagcacc accgcattcg ccgtcagcgg gtcgcgcgcg tattgaccaa ggcggacgtg
       61 catctgtccc agcaggtgga gcggccttga gggtgtgaag tcttcccacg cagttccgac
      121 gttcatatgg catggtctcc gtggtgcgaa tcagggttct accggcacgt cgtcaaggtt
      181 ctcaaagacc ttccgaatcg cgttcgccag cgcgttaatc tgttcgggcc cgttgggtgg
      241 acgatgatta aacactcccc aaagacacgt atcaatgcac tgctgcgaaa cgggatactg
      301 gctgagatcg taggagacgt ccttgcgggc aaacgcgctc gtccacggct cgcctttgcc
      361 gtatgcgttc ttcgcctgga acaccgcatg cgccggaagc agccagttcg cgatggagaa
      421 attcacacct tccgccttca atgcggccac gattccttcg gttatttttc gtagcggcac
      481 gccccgtctt tctgcgtacg caggctcgac gcgcagaacg taggcgtatc cgtttgtcgg
      541 gcggtcttcg gaagtaaagg agcggaccag attcggcgtc cctgccagac gctcgtcaag
      601 catatcccag ttgtcccgcg cccatttgtt cgtccggtca agtttccgga gctgcgaaag
      661 ggcaaacgcg gccgggagat cggacgttcg gtacatccat ccgagcccgt acgcgtggta
      721 gtctcttccg gaatccggcg ggcgcatatc cccgaaactc gtcaccattt tcccgcgttc
      781 gaaaagtccg gggtcattgg tgacgaaaag ccccccctca ccaccgcaca gattcttgtt
      841 ttgattgaga ctgaatgcag ccacgtcccc gattgtaccc accttcctcc ctttgaacgt
      901 cgcgccgtgc gcctggcatg cgtcctcgat cacggccaga ttatgccgtc tggcgatact
      961 catgatcgcg tccatgtcac acgccacacc ccagtaatga accacaagga tggcacgggt
     1021 atagggtgtg atcgcggctt cgatcttcga cgggtccatg tgcatgctgc gccagtcaac
     1081 gtccacgaag atcgggatgg cgttgtggtg gataatacac gtggcgctgc tcgtccacga
     1141 aagcgcggtc gtgatgacct cgtcaccggg cccgataccg catgcggcaa cgcacatgtg
     1201 aagcgcggcg gtgccgctgt tcgttgccat gcagaacgtg ttgccgttcc atgccgcgaa
     1261 ctccttctgc agcatgtccg catgcggccc gaacgcgtgg ctgtcctggt gcagtgacgc
     1321 gagcaccaac tcctcatcct ctttcgtgat ctccggccac atacggaact tgaggccggg
     1381 cggaaccgcg ggagttcccc cccgtattgc gagagtgtca gccatatcct atcctttgcg
     1441 aaacgacggc ccgggcacat tgcgagactc gggccggaaa aagtgtgatc tcgacatccc
     1501 ttagtatacc taactgaaag cggggcgcgg gagcaagacg atacacgcgc cggatgactc
     1561 gtgatcgagg cgaattcccg ttcatagtgc gaaaaaaagg actcgaatga ctcctcatct
     1621 gatctggcgg aaagaaacag gttaccagct caatgggatc gtgtccggga tggtggacgg
     1681 gcaggtgcgt ctctacctct ccggtattcg gactgtgtcc ggtcgtcgcg aatccgccat
     1741 attctgcctt tcctccgaag gcgaaaccgt gtggcagaca caggttgcgg acgcggcgga
     1801 ggaccgtctc gcctgcgtgt tcccgaacct gattgacgat ccatttctgg ggctgtgcct
     1861 cttctactcg tttggttcct ccgacaatgc ccgcccgcgc ccggggtatg caaggttggt
     1921 gcgcgcggaa agcggtgatg tggtgtggga attgcgcagc cgttcccatc atgcgggaaa
     1981 cgggtcgtgc atcgctgcgg atctggacgg cgacggaagg ccggaaatcg cgtggtggga
     2041 ttccgcccac gtcttctgtt ccgatctgcg gaccggtgat ctgcggtggg agtattccga
     2101 tcgcgtccag atctgccacg gcagacccgc gcttgcgcac ttgagaggaa tccccgccat
     2161 catcgtggga accgagtaca gcaatccgga cggcacctcg tccgtcctcg cgctcgacgg
     2221 atcaggcagg gtcctctggc ggaaggacgg attcccggaa gacctggggt ctacaccggt
     2281 catcctcgcg gatgtcgacc acgatggcag ggatgagttc gtcatcgcag gtcttgatct
     2341 cgtgcatcgc gacaacaggg aaacatcctc cctgtggtgt ttccgggatg acggcgaact
     2401 ggcgtacagg gttccgtgcg ggtgcggcgg gttggccgtc gccagagttc gaggggagtt
     2461 gctggccgcc ggtatcacca atacgcggga cggcgggaat aacggcgcgc aggaggttcg
     2521 actgttctcc cttgctaccg gagtgctgag gcactccgac cccttaccgc gcgcatacct
     2581 tgacgcacaa aatcccgttg ccgccgatct cgacggagac ggcctgccgg atttcctgct
     2641 caccactggc aacccgagcg ggtacggcaa ccgcccggcc atccggccgt acgcggacgt
     2701 gtatgtcgtc cgtgccgatg gcgcaacgct ctggacggaa acctttccgg actacattca
     2761 tcaaccctt
//
LOCUS       DAOTNG010000376         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_28712_length_2753_cov_5.021127, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000376 DAOTNG010000000
VERSION     DAOTNG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28712_length_2753_cov_5.021127"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1106)
                     /locus_tag="P5179_13390"
     CDS             complement(9..1106)
                     /locus_tag="P5179_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS96256.1"
                     /translation="MSGRQFNVWVFGDAHVGNDIRFGRESLADALRHSERGGEKGGDP
                     FPWDIAVDIGDMCGSQGLPEDGEGREVVRQFAALREHPREAVYSVCGNHDRSGLREPA
                     AWWWQKWVDPIGEHTQFSGVDPARRPFPVSGTWERYSFRVGNLLFLMMSDINEPTQTI
                     GRGDLGGNPGGVVSGETFRWWREMVESNPDHIIISVHHYMLKDTTVASGEWEGMDRDE
                     NGNWRGGYHGYKPQGTPIGASYLYWVDSQPDAQAFERYLAEHPGSVAIWLGGHTHAHP
                     DATDGGKTHIETKWGTHFINCGALTRYHNNVRHQNPPKSRLLTFTEGSDELRVRCYMH
                     TDEFLPQGWYDRAERRLRLGRANRGRAWKEA"
     gene            complement(1117..1878)
                     /locus_tag="P5179_13395"
     CDS             complement(1117..1878)
                     /locus_tag="P5179_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS96257.1"
                     /translation="MTNALSPSVQMQFMRPGQLEAAARAFPVVYVPFGLIEWHGRHLP
                     LGNDALKAHGILVKTAEKFGGVVYPPVYFPHDVNVPADITPEQAAADWRLCMLSALFE
                     RLKQTGFRVIIGVSGHNVQSQIDMVNQALKPVVADGTITGVGLWEISLSQCEESNTDH
                     AAKWETSNMQFFYPDLVDMAALGTAPLAPGMKPPDGIGGLDPREHASAEVGRRNVELA
                     SEAIGRKAQELLASLPSEHRAFRLPGISPEHWWAI"
     gene            complement(1934..2518)
                     /locus_tag="P5179_13400"
     CDS             complement(1934..2518)
                     /locus_tag="P5179_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS96258.1"
                     /translation="MGDLQETFHQWHHSPAHMFVPGSTYMVTAATYGKQPFFDTAAKK
                     DLLLAELFEQTKHYGWQLEAWAILNNHYHFVGKGGENAADLSRLIKRVHSETAVRINE
                     MDSTPGRKVWFQYWDSCLTHEKSYLARLHYVHQNPVKHRVVKDAEAYPWCSMGWFLRE
                     AEGGFRKTVLSFKCDRVNVLDDFQPLDCASSFAL"
BASE COUNT          454 a          929 c          814 g          556 t
ORIGIN      
        1 cgttcacgtt atgcctcctt ccacgcgcgg ccccggttcg cccgtcccag ccgcagcctc
       61 cgctccgccc ggtcgtacca gccctgcggg aggaactcgt ccgtgtgcat gtaacagcgc
      121 acgcgcagtt catcgctgcc ttccgtgaac gtgagcaggc ggcttttggg cggattctgg
      181 tgccgcacgt tgttgtggta ccgcgtcaac gcgccgcagt tgatgaaatg cgtgccccac
      241 ttcgtctcga tgtgcgtttt gccgccgtcg gtggcgtccg ggtgggcgtg cgtgtgtccg
      301 ccgagccaga tcgcgacgga accggggtgt tccgccagat agcgctcaaa cgcctgcgcg
      361 tccggctggc tgtccaccca gtagaggtac gacgcgccaa tcggcgtgcc ctgcggcttg
      421 tatccgtggt agccgccgcg ccagttgccg ttctcgtcac ggtccatgcc ctcccactcg
      481 cccgaggcca cggtggtgtc cttgagcatg tagtggtgca cactgatgat gatgtggtcc
      541 gggttcgatt ccaccatctc gcgccaccag cggaaggtct ccccgctcac cacgccgccg
      601 gggttgccgc cgaggtcgcc gcggccgatg gtctgcgtcg gctcgttgat gtcgctcatc
      661 atgaggaaca gcaggttgcc gacgcggaac gagtagcgct cccacgtgcc ggataccggg
      721 aagggccgcc gggccgggtc caccccggaa aactgcgtgt gctccccgat cgggtccacc
      781 cacttctgcc accaccaggc ggccggttct ctcaggccgc tccggtcgtg gttgccgcag
      841 acggagtaca cggcttcgcg tggatgctcc ctgagcgcgg cgaattggcg gaccacctcc
      901 ctgccttccc cgtcttccgg aaggccttgg gaaccgcaca tgtcgccgat gtcaaccgca
      961 atgtcccacg ggaacgggtc tccgcccttt tctccgcccc gctccgagtg ccgcagcgcg
     1021 tccgcgagac tctcccgccc gaagcgaatg tcgttgccga cgtgcgcgtc gccgaacacc
     1081 cagacgttga attgtcgacc gctcatctga tccccctcat atcgcccacc agtgctcggg
     1141 gctgatgccg ggcagccgga acgcgcggtg ttccgacggc agcgatgcca acaactcctg
     1201 cgctttgcgg ccgatcgcct cggatgccag ctccacgttg cgcctgccaa cctcggcgga
     1261 ggcgtgttcg cgcgggtcga ggccgccgat gccgtcgggt ggcttcatgc cgggtgcaag
     1321 gggggccgtg cccagcgccg ccatgtccac gaggtccggg tagaagaact gcatgttcga
     1381 cgtctcccat ttggccgcgt ggtcggtgtt cgactcctcg cactggctga ggctgatctc
     1441 ccacaggccg acgccggtga tggtgccgtc cgccaccacc ggtttcagcg cctgattcac
     1501 catgtcaatc tgcgactgca cgttgtggcc ggatacaccg atgatgaccc ggaaccccgt
     1561 ctgcttcagc cgctcgaaca gggccgagag catgcacagc cgccagtccg ccgccgcctg
     1621 ctcgggagtg atgtcggcgg gaacgttgac gtcgtgcggg aagtagacgg gaggatacac
     1681 caccccgccg aacttctccg ccgttttgac gaggatgccg tgggctttga gcgcgtcgtt
     1741 gcccagcggc aggtggcggc cgtgccattc aataagcccg aacggcacgt acacgaccgg
     1801 gaacgcccgg gcagccgctt caagctggcc cggccgcatg aactgcatct gcacggatgg
     1861 ggagagggcg ttggtcatgg cgtatgtcct ttctggaacg gtaccttgga gtgcgggagc
     1921 gccgctcccg ctttcatagc gcaaacgagc ttgcgcaatc caaaggctgg aagtcatcca
     1981 acacgttgac tctgtcgcat ttgaacgaca gtacggtctt tctgaaccca ccttctgctt
     2041 ccctcaaaaa ccaccccatc gaacaccacg gatacgcctc agcgtctttc acaacccggt
     2101 gtttgacagg attctggtga acgtagtgca acctcgcgag atagctcttc tcatgggtca
     2161 ggcagctatc ccagtactga aaccacacct ttcgccccgg ggtactgtcc atttcgttga
     2221 tccggacggc cgtttcggaa tgaactcgtt tgatcagtct cgacaaatcc gctgcgtttt
     2281 ctcccccctt gccgacgaag tgatagtggt tattcagaat agcccacgcc tccagctgcc
     2341 atccgtagtg ctttgtttgc tcgaacaatt ctgcaagcag gaggtccttt ttcgcggcag
     2401 tgtcgaaaaa cggttgcttc ccgtacgtcg ctgcggtgac catgtaggtg ctgccgggaa
     2461 cgaacatgtg cgcgggcgag tggtgccact ggtggaaggt ttcttgaagg tcgcccattg
     2521 cccacgcctc ttctttggag tgcgggagcg gcgctcccgc tttcacagcg caagcccggc
     2581 ttgcgcattc caaagtacga cggcgccctt ggcaatccca gccacatccg ccaatttcca
     2641 ataccaacac caggacgcat cgtgaatcgc caaaggacct ttccccatgt cccgatttcc
     2701 cgttgaccac gtgtaactcg tacacacctt tccccccgac ccccaatctt atc
//
LOCUS       DAOTNG010000377         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_28988_length_2733_cov_4.930919, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000377 DAOTNG010000000
VERSION     DAOTNG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28988_length_2733_cov_4.930919"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="P5179_13405"
     CDS             <1..1414
                     /locus_tag="P5179_13405"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRS96259.1"
                     /translation="RDLKGEEMHKSKGNAIWFDDAAEKMGAETMRWLYCRQNPYTNLN
                     FGYEAAEEPLRHLTTLWNTYKFFVTYAKIDGFVPGSSPVPYDRLDRMDRWILARLNQL
                     IADARRAFDGFSVMNFVHAAERFIDDLSNWYVRRCRDRFRQADQAGDPAAYETLYTVL
                     STTVRLLAPVIPMFAEELYQNLVAWREGEPESVHLTPYPEETPEWEDAALVADMALVR
                     NVVEIALAARNAAKIKVRQPLAVMTVVTDPSAIKTLEANRQVIAEELNVKRIVFTDSA
                     EGLTTRVAKPVFRALGPRFGKDANAVGNILRALDQDTLARIAAGETVTIPVNGKHEQI
                     SSELVEVVTASPQGMSVVSDKQVTVALDTNLSDDLVDEGFAREIVNKIQNMRKEADFN
                     VSDRITVGYSVSPRLARAIDSCRTYIMKETLARELVPLEGEWEHRSEWSVNGEPAELG
                     VRRTTQSGLANGGGMQDGRT"
     gene            1401..1790
                     /locus_tag="P5179_13410"
     CDS             1401..1790
                     /locus_tag="P5179_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRS96260.1"
                     /translation="MAALDPQELAYFEKLLKERRAKALSAAEEGESVRPKEAPKESSG
                     DLSAYSVHMPDQGTDAMERELEFLLAARDEKYIRHLDEALQRIKDGTFGICRTCNKPI
                     SRARLEAVPNATECIACKSQRDKLPRR"
     gene            1825..2340
                     /gene="lspA"
                     /locus_tag="P5179_13415"
     CDS             1825..2340
                     /gene="lspA"
                     /locus_tag="P5179_13415"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRS96261.1"
                     /translation="MFIGPALASLGLDRITKIAAERHLTFSVPEKVLGEYVRVVLWFN
                     EGAAFGMTFGGKWAHVILSLIAMVVVTYLVWRTPREHSLMLSGFALILGGAAGNLWDR
                     LLAGKVTDFIDVGIGSLRWPTFNVADASVVVGVFLILLTSWRTSRAEQTAKETTAHPA
                     QTAATEGTPVP"
     gene            2337..>2733
                     /locus_tag="P5179_13420"
     CDS             2337..>2733
                     /locus_tag="P5179_13420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRS96262.1"
                     /translation="MKTPLRAADYRLLLSLGLGVLFTDWLTKALAANLLSAGRPVSLF
                     GDYARLSLSHNPGAAFGMRFGGAAVHLLVTVAVIVFIAAIALHTAPSRKLEQAGYGLI
                     LGGAIGNLGDRLLLGHVIDFLDLGIGNVRW"
BASE COUNT          563 a          809 c          816 g          545 t
ORIGIN      
        1 gcgcgatctc aaaggcgagg agatgcacaa aagcaagggc aacgcaatct ggtttgacga
       61 cgccgccgag aagatgggcg cggagaccat gcgttggctc tattgccgcc agaatccgta
      121 tacgaacctg aatttcggct acgaggcggc ggaggaacct ctgcggcatc tcaccacact
      181 ctggaacacc tacaagttct tcgtaacgta cgcgaaaata gacggcttcg ttccgggcag
      241 ctccccggtg ccctacgacc ggctcgatcg aatggatcgc tggattctcg cccggctgaa
      301 ccaactgatt gccgacgcgc gccgtgcgtt cgacgggttt tccgtgatga acttcgtaca
      361 cgcggcagag cgattcattg acgacctttc caactggtac gtccgccggt gccgggatag
      421 attccgacag gcggatcagg cgggagaccc ggccgcgtac gagacgttgt acaccgtcct
      481 ctctacgacg gttcgacttc tcgcgcccgt gatcccgatg tttgcggaag agctgtacca
      541 gaacctcgtt gcgtggcggg aaggggaacc cgaaagcgtg catctgacgc catacccgga
      601 agagacgccc gaatgggagg atgcggccct cgttgccgat atggcgctgg tgcggaacgt
      661 ggtggaaatc gcgcttgccg cccggaatgc cgctaaaatc aaagtccgtc agccgcttgc
      721 cgtgatgaca gtcgtcaccg atccgagtgc aatcaaaacg ctggaagcga accgtcaggt
      781 gatcgctgag gaacttaacg tcaagcggat cgtctttact gattccgccg aagggctcac
      841 cacgcgcgtg gcgaaaccgg tgttccgcgc gctcgggcca cgctttggaa aagacgcgaa
      901 cgccgtgggg aacatcctcc gcgcactcga ccaggatacg ctcgccagaa tcgcggcggg
      961 cgaaaccgtg accattcctg tcaacggaaa acatgaacag atatccagtg aacttgtgga
     1021 ggtcgtcaca gccagcccgc agggcatgtc ggtagtttct gacaagcagg ttaccgtggc
     1081 cctcgacacg aacctgtcgg acgaccttgt ggacgaaggg tttgcgcggg aaatcgtgaa
     1141 caagatccag aacatgcgta aggaagccga tttcaacgtc tctgaccgga tcaccgtggg
     1201 ctactccgtc agtccgcgcc ttgcacgtgc gatagattct tgcagaacgt acatcatgaa
     1261 ggagactctt gcgcgtgaac tggtgccgct cgaaggcgag tgggagcacc gctctgaatg
     1321 gtcggtgaac ggagaacccg cggaacttgg agttcggcgc accacgcaat caggcttggc
     1381 aaacggtggg ggaatgcaag atggccgcac ttgacccgca ggaactcgcg tatttcgaga
     1441 agctgttgaa agaacggcgg gcaaaggcgc tttccgctgc agaagaggga gaaagtgtcc
     1501 gcccgaaaga ggcgcccaag gaatcttcgg gagacctgag cgcctattcc gttcacatgc
     1561 ccgatcaggg aacagatgcc atggagcgcg agctggaatt cctcctcgcc gctcgcgatg
     1621 agaaatacat ccgtcatctg gacgaggcac tccagcgcat caaggacgga accttcggca
     1681 tctgccgtac ctgcaacaaa ccgatatccc gtgcacgcct ggaagcggta ccgaatgcca
     1741 cagaatgcat tgcctgcaaa tcacagcgag ataagctccc ccgacgctga aacgcgcccc
     1801 ttctcatgga caagggcgtc ctgcgtgttc ataggtcccg cactggcttc gttgggtctg
     1861 gatcgtatca ccaaaatcgc cgcggaacgt catctcacct tttccgttcc ggagaaggtg
     1921 cttggcgaat acgttcgcgt cgtcctctgg tttaacgaag gcgccgcgtt cggaatgaca
     1981 ttcggtggca aatgggcgca tgtcatcctt tccctgattg cgatggttgt tgtcacctac
     2041 ctcgtctggc ggactccgcg cgaacactcc ctcatgcttt ctggttttgc cctcatcctc
     2101 ggaggcgcgg cgggcaatct gtgggatagg ctccttgccg gcaaagtcac cgatttcata
     2161 gacgtcggca taggttccct gcggtggccg accttcaacg tggcggacgc cagcgtggtg
     2221 gtcggggtgt tcctcatact ccttacaagc tggagaacca gccgcgcgga gcagaccgca
     2281 aaagaaacta cggcgcaccc cgctcagact gcagcaactg aagggactcc cgttccgtga
     2341 aaacgcccct gcgcgcggcc gattaccggc tcctgctgag cctcggcctc ggtgtgcttt
     2401 tcaccgactg gttgaccaaa gcgcttgcgg cgaatctcct ttccgccggc cgtcctgtct
     2461 ctcttttcgg ggattacgcg cgattgtccc tgtcgcacaa tccgggggcc gcgttcggga
     2521 tgcggtttgg cggtgcggct gtgcacctgt tggtcaccgt cgcggtgatc gtgttcatcg
     2581 cggcaatagc cctgcacacc gctccctcca gaaagcttga gcaggccggc tacggtctta
     2641 tccttggcgg cgccatcgga aacctcggtg atcgcctcct gttgggtcac gttattgatt
     2701 ttctcgatct cggtatcggc aacgtgcggt ggt
//
LOCUS       DAOTNG010000378         2725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_29083_length_2725_cov_4.779401, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000378 DAOTNG010000000
VERSION     DAOTNG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29083_length_2725_cov_4.779401"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..539)
                     /locus_tag="P5179_13425"
     CDS             complement(279..539)
                     /locus_tag="P5179_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRS96263.1"
                     /translation="MRSKTHGVSTSDVEVSNISPTGVWLYARGREYFLPFDEYPWFRN
                     ATVGAIHNVELHHGIHLRWPDLDVDLEMDSLSSPEKYPLRFQ"
     gene            complement(499..738)
                     /locus_tag="P5179_13430"
     CDS             complement(499..738)
                     /locus_tag="P5179_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRS96264.1"
                     /translation="MSPTIFRHGEFRFLFFSREEERVHVHVMGTTGEAKFWLDPKVEL
                     AVNHGLSTGEIREITAIIEGKRREIEDAWREHFGR"
     gene            complement(877..2403)
                     /locus_tag="P5179_13435"
     CDS             complement(877..2403)
                     /locus_tag="P5179_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HRS96265.1"
                     /translation="MLETVDLSKELDKATYDEWFPKLQDNLRVLQREAFEAGMTTLIV
                     LEGWDASGKGDAVMKLVDKLDPRGFRVDPPAPPSEDEKLRPFLWRFWVKLPPYGAIGI
                     FDRSWYGRVLVERMDGMLQPRQWQQAYQEINQFERQLSDDGQVIIKLFLHISKKEQAK
                     RFKQMEKDPAESWKVTKEDWKHHKQYDDYLRVNEEMLERTSSSYAPWTIIEATDRRYR
                     RVKIFQTVIQAMQAGLERKKFLDARRAEEKEAFKKGLKNGNEQALPLDVMPSILDRVD
                     TSLTYDPEAYKKDLAKLQVRMRELEFECFKHRLPVVIGYEGWDAAGKGGNIKRVTQTL
                     DPRGYNVVPIAAPKGDEATHHYLWRFWRHIPKAGHITIFDRTWYGRVLVERVEGFTAK
                     SDWQRAYQEINEFEQSLVNWGAVVLKFWIHISKEEQLRRFEERQVTEYKQYKITEEDW
                     RNREKWDAYKAALTDVFERTSTSYAPWTIVEGNDKLYARIKTLKTIIGAIEGKIGGKK
                     "
BASE COUNT          503 a          821 c          764 g          637 t
ORIGIN      
        1 cggggtgcgg ctctttcggc ccgccgctgt acagggctgc ccagaggcgg ccgttggcgg
       61 cgtactcgat gccggggatg ccctgccaga gtcggtcctg cttcgttgcg atcaggtgtg
      121 tgcgcacggg gaatcctttc gcgggcgtgg aaaaaggcgg cgttccggta cccccgaaag
      181 ggaatgcaaa gacgccggtg agtcaaggaa caagcaggtc gcgcgaaacg acgcggtgtg
      241 caacgaacca acaaaatccg tcgccaagca gcacggcgtc actggaatct gagagggtac
      301 ttctccggag aactcagcga atccatttcg agatcaacgt cgaggtcggg ccagcgcagg
      361 tggataccgt gatgaagctc cacgttgtgg atcgctccta ccgtcgcatt ccgaaaccac
      421 gggtactcgt cgaaaggaag gaaatattct cggccgcgtg catacagcca cacgccggtg
      481 ggcgagatat tgctgacctc aacgtccgaa gtgctcacgc catgcgtctt cgatctcacg
      541 tcttttcccc tcgatgatcg ctgtaatttc ccgtatctct ccagtagaga gaccgtggtt
      601 gaccgcaagc tcgaccttcg gatccagcca gaacttggct tcgccggttg tgcccattac
      661 atgcacgtgg actcgttcct cttcgcggga gaagaacaaa aaccggaatt caccgtgcct
      721 gaatatcgtc gggctcatgc cattagtatc tcactttttc cgttacggcg caaatccgaa
      781 cgttcttcct gttcgagacg ccacacccgc accacacgac aagggggcgc cttgcgggcg
      841 ccccctcaga gaatcagccg gaccgcgtcc gcgggtctat ttctttccgc cgattttccc
      901 ctcaatggct ccgataatgg ttttgagcgt tttgatgcgc gcgtagagct tgtcgttgcc
      961 ctcgacaatc gtccacggcg cgtagctggt gctggtgcgc tcaaacacat ccgtaagagc
     1021 ggccttgtac gcatcccatt tctcgcggtt gcgccagtct tcttccgtga ttttgtactg
     1081 cttgtattcg gttacctgcc gttcttcgaa ccggcgaagc tgctcttcct tgctgatgtg
     1141 aatccagaat ttcagcacca cggcgcccca gttcaccagg ctctgttcga actcattgat
     1201 ttcctggtac gcccgctgcc agtcgctttt cgcggtgaat ccttccaccc gttccaccag
     1261 aacacgcccg taccacgtgc ggtcgaagat ggtgatgtgc ccggctttcg gaatgtgtcg
     1321 ccagaagcgc cagaggtagt ggtgagttgc ctcatcaccc ttcggcgctg cgattggcac
     1381 gacgttgtat ccgcgcggat cgagcgtctg tgtcacccgt ttgatgttgc cgcccttgcc
     1441 cgcggcgtcc catccttcgt agccgatcac cacggggaga cggtgtttga aacactcgaa
     1501 ctcgagttcg cgcatccgca cctggagctt ggcgaggtcc ttcttgtacg cctcgggatc
     1561 gtaggtgagc gaggtatcca cccggtcgag gatggacggc atgacgtcca gcggaagggc
     1621 ctgttcgttc ccgttcttga ggcctttctt gaacgcttct ttctcttccg ctctccgtgc
     1681 gtccaggaat ttcttcctct ccagacccgc ctgcatggcc tgaatgacgg tctggaagat
     1741 tttcacgcgg cgataccgac gatcagtcgc ttcgatgatc gtccacggcg cgtagctgga
     1801 actcgtgcgc tcgagcattt cctcgttgac gcgcaggtaa tcgtcgtact gcttgtgatg
     1861 tttccagtcc tctttggtga ctttccacga ttcggcagga tccttttcca tctgcttgaa
     1921 gcgttttgcc tgttcctttt tgctgatgtg caggaacagt ttgatgatca cctgcccgtc
     1981 gtcggaaagt tgccgttcga actgattgat ctcctgatac gcctgttgcc actggcgcgg
     2041 ctgcaacatc ccgtccatgc gctcgaccag cacacgtccg taccacgagc gatcgaagat
     2101 cccgattgcg ccgtagggag ggagtttgac ccagaaccgc cagaggaacg ggcggagttt
     2161 ctcgtcctca ctgggaggtg ccggtggatc cacgcggaag ccgcggggat ccagtttgtc
     2221 caccagcttc atcaccgcat cgcctttgcc ggaggcgtcc catccctcga gtacgatgag
     2281 cgttgtcatc ccggcttcga aggcttcccg ctgcaacacg cggagattgt cctgcagctt
     2341 cggaaaccat tcgtcgtacg tcgctttgtc gagttctttt gacaggtcaa cagtttccag
     2401 catgtccccc tccttggaag gtatggcgga attccgtgat catgtgacaa gcaactcgaa
     2461 gcggttgata ggtacaaatc ggcacgtcaa gggtaccaca tttcgtacga caaggcaaac
     2521 gcctgggatt ggatgagaac cgtggagaga ttactcgttt ccggaaacgc cgcacgcgcg
     2581 gttgagcaag cgcgccatct ggcccgtctg gcttctgcgg gagaggtgtt ccacgccctc
     2641 catcggtgcg cgcacaggta gcgagttacg tttccagaga ccaaacagac gctccagttc
     2701 cgcccggatt gcccccacgt cgtct
//
LOCUS       DAOTNG010000379         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_29321_length_2708_cov_4.282322, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000379 DAOTNG010000000
VERSION     DAOTNG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29321_length_2708_cov_4.282322"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="P5179_13440"
     CDS             <1..669
                     /locus_tag="P5179_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96266.1"
                     /translation="DGRPVAPPTYAGFFTQGPFELAGHLRGTTELCVDMYDDPAYFHD
                     LMDFCTRAIIVRVKAIFGDLLGVPMPFEHFAYTDDSIAMLSAEAFQKNVWPYHKRMFD
                     ELATPTCTHFLHLCGTVQQHIPFLASTGLINYLGTSYPVDIPRARREAGDDLWLDTAI
                     HADIVLRGSAEAIDAAVAAKLTPEAKGKGRLVFMGGNYIAPGTTLDKFLMLYESVKRH
                     GMYR"
     gene            683..1768
                     /locus_tag="P5179_13445"
     CDS             683..1768
                     /locus_tag="P5179_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRS96267.1"
                     /translation="MAGFNGLGMNLGNLSRLSTAKTRSISAENFTGEKGKGGMAKEGT
                     GANAARDLGQGWKISPCVRVKAGETFEMANIEGPGAIQHIWLTPSGHWRFQILRIYWD
                     NAKTPSVECPVGDFFACGWNQYAQVSSLPVCVNPGRALNCYWEMPFRKRCRMTMTNIG
                     KEDATLFYQVDYTLTDVPDDCAYFHAQFRRTNPLPYKEVYTIVDGIKGRGQYVGTYMA
                     WGVNNCGWWGEGEIKFYMDGDKKFPTICGTGTEDYFCGSYNFDVGKENGGYREFTTPY
                     AGLPQVIRPDGLYSSQQRFGLYRWHIMDPIRFEKDLRVTIQALGWRSGGRYLPNQDDM
                     ASVAFWYQTLPAAPFPKLPDADYLEVI"
     gene            complement(2157..>2708)
                     /locus_tag="P5179_13450"
     CDS             complement(2157..>2708)
                     /locus_tag="P5179_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS96268.1"
                     /translation="AITRVETLRDLRLGEFDVLVGINLLREGLDLPEVSLVAILDADK
                     EGFLRSERSLIQTAGRAARHVNGQVIFYADVVTDSMRRAISETNRRRELQLAFNEEHG
                     ITPRSIVKSLDQVMAQTAVADSRPRIEPKVHVPVVRGKDLEATIRELERLMLEAAKDL
                     EFEKAAAIRDEIADLKKQAGNGK"
BASE COUNT          515 a          843 c          841 g          509 t
ORIGIN      
        1 gacggccgcc cggttgcccc acccacctat gccggattct tcacacaggg cccgtttgaa
       61 ctcgccggcc atctccgtgg aaccaccgag ctctgcgtgg acatgtacga cgatccagcg
      121 tatttccacg acctgatgga tttctgcacc cgggccatca tcgtgcgcgt aaaggccatt
      181 ttcggcgatc tgctcggcgt tcccatgcca ttcgagcatt ttgcgtacac cgatgacagc
      241 atcgccatgt tgagcgcgga ggcattccag aaaaacgtct ggccctatca caagcgcatg
      301 tttgacgaac tggcgacacc cacgtgcacg catttcctgc acctgtgcgg aaccgtgcag
      361 caacacatcc ccttcctcgc aagtacgggg cttatcaact acctcggcac cagctacccg
      421 gtggacatac cccgcgcccg gcgagaggcg ggcgacgacc tctggctcga caccgcgata
      481 catgccgaca tcgtcctgcg cggctccgct gaggccatag atgcagccgt tgcggcgaaa
      541 ctcacgcctg aggcgaaagg gaagggccgg ctggtgttca tgggcggcaa ctacatagcg
      601 ccgggaacga cgcttgacaa gttcctgatg ctctacgaat cggtgaaacg acacgggatg
      661 taccgctgac aaggaggaac aaatggcggg attcaacgga cttggtatga acctcggtaa
      721 cctctcgcgc ctctccacgg caaaaacacg ttcgataagc gcggagaact ttacgggcga
      781 aaagggcaag ggcgggatgg cgaaggaggg taccggcgcc aatgcggcac gggacctcgg
      841 tcagggctgg aagatctccc cgtgcgtgcg ggtgaaggcg ggcgagacat tcgaaatggc
      901 gaacattgaa ggccccggcg caattcaaca catctggctc accccgagcg ggcactggcg
      961 gttccagatt ctccggatct actgggataa cgcaaaaacg ccgtcggtgg agtgcccggt
     1021 gggtgacttc ttcgcgtgcg gctggaacca gtacgcgcag gtctcttccc tgccggtgtg
     1081 tgtgaatccg ggtcgcgcgc tcaactgcta ctgggagatg cctttccgca agcgctgccg
     1141 tatgacaatg actaacatcg gcaaggaaga tgccaccctg ttttatcaag ttgattacac
     1201 gctcaccgac gtgccggacg attgcgccta ctttcacgcg cagttccgcc gcaccaatcc
     1261 actgccgtac aaagaggtct acaccatcgt ggatggcatc aaaggtcgcg ggcagtacgt
     1321 cggcacgtac atggcgtggg gagtgaacaa ctgcggctgg tggggcgagg gcgagatcaa
     1381 gttctatatg gacggtgata agaagtttcc taccatctgc ggcaccggca ccgaggatta
     1441 tttctgcgga tcatacaatt tcgatgtcgg caaggagaac ggcggctacc gcgagttcac
     1501 cacgccgtac gccggcctcc cgcaggtgat ccgtccggac gggctctatt cctcgcagca
     1561 gaggttcggc ctctaccgct ggcacatcat ggacccgatc cgctttgaaa aggacctgcg
     1621 cgttaccatt caggcgctgg gctggcgttc aggcgggcgt taccttccca atcaggacga
     1681 catggcatcg gtggcattct ggtaccagac cctgcccgcc gccccgttcc cgaagctgcc
     1741 ggatgccgat tatctggaag tgatctgatc gcggagggga aaagggacgc gacgaagcgc
     1801 gtccctccat agcgagcgcc gcaggaggag gacacgcaca cagactggcg gggcccgctc
     1861 tgtcgggccc ttgttttgtt cctcattcac cccgttcttc ccgcggccgt cttcgagtga
     1921 cgaagcggat ggcggttctg cgccggggcg cagtccgggg aaacagggtt ggttcggggg
     1981 ccgtgggcgc ttcgcgaagc gcccctgcga tggcccgggc gcggcggttc ccatgcgtcc
     2041 cataggacct atggcccggg ggacggcacg gcggtggtct gggaacaaac caccctagca
     2101 tgtgcattcc cggcggcagg ggcacggttg ccgtgggccg ctccaccggt cccgcgtcac
     2161 ttcccgtttc cggcctgctt cttcaaatcg gcgatttcgt cccggatggc ggcggctttc
     2221 tcgaattcga ggtccttggc ggcctccagc atcagccgct ccagttcgcg gatggtggcc
     2281 tccagatcct tccccctcac cacaggcacg tgcaccttcg gctctatcct gggacgtgag
     2341 tccgccacgg ctgtctgcgc catcacctga tccagcgact tgacgatcga gcgcggggtg
     2401 atgccgtgtt cctcgttgaa cgcgagctgg agttcgcgcc gccggttggt ttcgctgatg
     2461 gcccggcgca tggaatcggt gaccacgtcg gcatagaaga tcacctgccc gttcacgtgg
     2521 cgcgcggcgc ggccggcggt ctggatgagc gagcgttcgg agcggagaaa gccctccttg
     2581 tccgcgtcga ggatggcaac gagcgatacc tccggcagat caagcccttc gcgcagcaga
     2641 ttgatcccga ccagcacatc gaattcgcca aggcgaagat cgcgcagagt ttccacccgg
     2701 gtgatggc
//
LOCUS       DAOTNG010000380         2694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_29542_length_2694_cov_3.814324, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000380 DAOTNG010000000
VERSION     DAOTNG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29542_length_2694_cov_3.814324"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="P5179_13455"
     CDS             complement(<1..998)
                     /locus_tag="P5179_13455"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS96269.1"
                     /translation="MHARTMVAFLLLHCGVCAASDSFVIARNGRAIAKIVTAPEIDRP
                     FDKGVNNQAVRDLIRVIGVMSGASLPAVQDTLAFNDGPQIHVGQTEFVKRAGLIEPGL
                     PANAYRVVLTYEGEQARLVLAGPTALGTSNAVYDLLTRELGVIWGMENQLFEDIPRRQ
                     TVEVGPVDRTVRPPFPFFFLSGPSAPWQARHRLSSGGLEFPYMRHGHNVYHYFPVSRY
                     RNHPEYYPLVDGRRKLPERDGDGRAHVCYTNPEVVAIMIEAVRWYFDANPQAPGFSLC
                     AADDDGMCSCDNCSRLDNGAPPFRGRERIYSDSYFAFVGTVAKAVLQSHPDRYLSA"
     gene            complement(1131..>2694)
                     /locus_tag="P5179_13460"
     CDS             complement(1131..>2694)
                     /locus_tag="P5179_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRS96270.1"
                     /translation="WTLSHYGKFFSFNSPANLLATWNSVLVSCLSVILAALIGVPLAF
                     LLGTFEFRGRKACSALVSMPIVLPPLVGVLAFVFLLGESGLVPRGLSNLTGSNSASWA
                     LNGIAGVVAVHGYSFSVFFFTFVSSALENRDTSLMEAAASLGAGKWLRLRRVTLPLLM
                     PALVGAAIIVFMTSMASFSAPLLFAGELRVLSVQLYSTRLSGDLRLAAVQSVVLSGVS
                     LAFLLALRYWNNRRRVTMATKGTRGRRRSLPRGLTRWLIPSAGFVVSVIMVLPHLTLV
                     LLSFIKQGTWTFRSDAWFPAVFTVENYRMALSLARPLANSIQMAFFATLGNLVIGVFI
                     GWLLAVKRIRFGNAVDALVMLPWALPGTVVAVNLLTAFSTGNVFSFGQVLAGSVWLLP
                     LAYFVRNLPVVARSAQSAFAQIDPSLEEAARSLGAGWWLRFRRVTLPLIAPGVLGGAM
                     LAFVTALGEFVASVLLWVPANQPISMAIFGEYREYRLEVAAAYGVLLVAFIGLVFFVS
                     RVAFGSKAASEV"
BASE COUNT          659 a          832 c          755 g          448 t
ORIGIN      
        1 tacgcgctca ggtaccggtc agggtggctt tgaagcaccg ctttcgccac tgtacccaca
       61 aatgcgaaat agctatctga atagattctt tccctgccac gaaacggagg cgcaccgttg
      121 tccagccgcg agcaattgtc acacgagcac atcccatcgt cgtcagcggc acaaaggctg
      181 aaaccaggcg cttgaggatt tgcgtcgaaa taccaccgaa ccgcctctat catgattgca
      241 accacttctg gattggtata acagacgtgg gcgcgtccat caccatctct ttccggcagc
      301 ttgcgccgcc cgtcaaccag cgggtaatac tccgggtgat tgcggtaccg gcttacagga
      361 aagtagtggt agacgttgtg gccgtgccgc atgtacggaa actccaggcc acccgatgaa
      421 agccggtgcc gcgcctgcca cggcgcagaa ggcccgctca gaaagaagaa ggggaaaggg
      481 ggtcggacgg tgcggtctac agggcccact tcgactgtct ggcgccgcgg gatgtcctca
      541 aaaagctggt tctccatgcc ccagattacg cccagctcac gggtcagtag atcgtacacg
      601 gcgtttgacg tgccaagggc agtcggtccc gcgagcacca accgggcctg ttctccctca
      661 tacgtcagca ccacgcgata cgcgttcgct ggcagccccg gttcgatgag ccccgctcgt
      721 ttgacgaatt ccgtctgccc gacatgtatc tgcggaccgt cattgaaggc cagcgtgtcc
      781 tgaaccgccg gcaatgacgc gccagacatt actccgatca cgcgtatcag gtcccgcacc
      841 gcctgattgt tcacgccttt gtcaaacggg cgatcgattt ccggcgcggt cacgattttc
      901 gcaatggcac gcccgttccg agcaatgacg aaactgtcgg atgctgcgca aaccccgcag
      961 tgaaggagca gaaatgccac catggttcgc gcatgcatca cgtcacctca cctccgacca
     1021 gccggttggc aatctccaca aagatcactt gaggggcatc cggggacaac cgcaatgaga
     1081 gaagcacgcg tgataatgct aattatgtct agttgactcc ggtccattgc tcacacttct
     1141 gacgctgcct tcgatccgaa cgcaacccgg gagacgaaaa acaccagccc gatgaacgcc
     1201 acgagcaaca ccccgtacgc cgcggcgact tccagtcggt actcgcggta ttccccgaaa
     1261 atcgccatgg atatcggctg gtttgccgga acccagagca acacggacgc aacgaattcc
     1321 ccgagggcag ttacgaacgc aagcatcgcc cccccgagaa cgcccggagc aatgagggga
     1381 agcgtgacgc gacggaaacg caaccaccat ccggcgccga ggcttcgcgc ggcttcctca
     1441 agtgaggggt cgatctgggc gaacgcgctt tgcgcggagc gggcaaccac gggaaggttt
     1501 cgaacgaaat aggcgagggg cagtaaccag accgaacccg cgagaacctg cccgaagctg
     1561 aagacgtttc cggtgctgaa cgcggtaaga agattgacag ctaccaccgt ccccggaagc
     1621 gcccacggca acatgaccag cgcgtccacc gcgttcccga accggatacg cttcactgcc
     1681 agcaaccacc ctatgaacac accgatcacc agattgccga gtgtcgcgaa gaacgccatt
     1741 tgaatgctgt ttgcaagcgg gcgcgccaga gacagcgcca tccggtaatt ctcgaccgtg
     1801 aatactgcgg gaaaccacgc gtcggaccgg aaagtccacg ttccttgttt gatgaaagac
     1861 agcagtacta acgtcagatg gggcaaaacc atgatcaccg aaaccacgaa cccagcgctc
     1921 gggatcaacc atctggtcaa gcctcttggc aggcttctcc gcctgcctcg tgttcctttg
     1981 gttgccatgg tcacgcgacg gcggttgttc cagtaccgca gcgcaaggag aaacgcaagg
     2041 gataccccgg aaagtacgac agactggacc gcagcaagcc tgagatcacc cgaaaggcgg
     2101 gtggagtaga gctggacact caacacacga agttcaccgg cgaacaacaa cggagcgctg
     2161 aatgacgcca tgcttgtcat gaacacaatg atcgcggcgc cgaccagcgc aggcatcaga
     2221 agtggcaacg tcacgcgacg gagcctgagc cactttccgg ctccgagact ggctgccgcc
     2281 tccatgagac tggtgtcacg gttctccagc gcggaactta caaacgtgaa aaagaagact
     2341 gagaagctgt acccgtggac tgcaaccacc ccggcgattc cgttcagcgc ccacgatgcc
     2401 gagttggatc ccgtcaggtt cgaaagcccg cgcggaacca acccgctctc gcccagcaaa
     2461 aaaacaaagg caagtactcc cacaagtgga ggcaagacaa tcggcattga gacgagtgcg
     2521 ctgcaggcct tcctgccccg gaactcaaac gtgcccagga gaaaggccaa aggcacgcca
     2581 atgagtgcag ccagaatgac ggaaaggcag gaaacgagga cgctgttcca tgtcgcgagg
     2641 agatttgcgg gagaattgaa cgaaaagaac ttgccgtaat gcgaaagcgt ccac
//
LOCUS       DAOTNG010000381         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30406_length_2634_cov_4.702986, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000381 DAOTNG010000000
VERSION     DAOTNG010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30406_length_2634_cov_4.702986"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="gcvPB"
                     /locus_tag="P5179_13465"
     CDS             <1..1174
                     /gene="gcvPB"
                     /locus_tag="P5179_13465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRS96271.1"
                     /translation="FVEMHPLAPPQTTQGLLRLIHELERMLCEITGFSGITLQPPAGA
                     ASELTGLLVMRQYHRKKGNRKTHVLLPDSAHGTNPASATFAGYEARQLASTEDGRLSL
                     DALRAAVNDETAGLMITNPSTLGLFETHIREIADIIHGVDGLVYMDGANMNALLGIAK
                     PAATGVDMCHLNLHKTFSTPHGGGGPGGGCLAVREDLVPFLPVPRVEHGEGDYRLAWE
                     RPDTIGNVHAFCGNVGVMIRAYCYIRMMGGEGLERVSRTAVINANYLYALVKDLFDTP
                     YQGPYMHEFVVSGDRQKARGARTLDIAKRLLDFGVYAPTVYFPLIVSEAMMIEPTETE
                     SKQAIEEYAAFLRQIAREVDEEPETVLKAPHTTPIGRVDEVRAARQPDLRYGFQGV"
     gene            1362..1796
                     /locus_tag="P5179_13470"
     CDS             1362..1796
                     /locus_tag="P5179_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96272.1"
                     /translation="MPLVERRFSPAVVYVTVCTKDRKAILHRPEINTLLGEAWQSAEG
                     WLVGRYIIMPDHLHLFCAPATLEYPELAKWVKCWKAFVSLNWPVRGEQPIWQRSFWDT
                     QLRSAEDYENKQDYMVHNPVRAGLCATPGNWPFQGELNVLYW"
     gene            1978..>2634
                     /locus_tag="P5179_13475"
     CDS             1978..>2634
                     /locus_tag="P5179_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96273.1"
                     /translation="MYPAYKTHLFSSELFRRAEEARRFLAPCRVCPRACGVDRLAGKT
                     GVCGIGAKATVASALAHHGEEPGLSGARGAGTIFFGGCNLRCIFCQNHQISQTSHSPE
                     YGPEEIAAMMVRLQGEGCHNIDLVSPSHVAPQVLEAIARAAEQGLSIPILYNSNGYDS
                     LDMLRLFDGVADIYLPDLKYGDDSTGTWASSVDNYVEHAHAALKEMYRQVGDVEFDNN
                     G"
BASE COUNT          531 a          829 c          796 g          478 t
ORIGIN      
        1 tttcgtggaa atgcacccgc tcgcccctcc gcagacaacg cagggactct tgcggttgat
       61 tcatgaactg gagcgcatgt tgtgcgaaat tacggggttt tccggaatca cgcttcagcc
      121 ccctgccggc gcggcaagcg aactcaccgg acttctggtg atgcggcagt accatcggaa
      181 gaaaggaaac cggaaaacgc acgtcctgct gccggattcc gcccacggca cgaaccccgc
      241 atccgccacc ttcgccgggt acgaggcccg tcaacttgcc agcaccgaag acggtcggct
      301 ttcactcgac gctctgcgcg cggccgtgaa tgacgaaacg gccggcctca tgatcaccaa
      361 ccccagcacg ctgggactct ttgaaacaca tatccgcgag atcgccgaca tcatccacgg
      421 ggtggacgga ctcgtctaca tggacggtgc gaacatgaac gcgctgcttg gaatcgccaa
      481 acctgcggcg acgggcgtgg atatgtgcca cctcaacctg cacaagacct tctccacccc
      541 gcacggagga ggcggaccgg gcggcggatg ccttgccgtc cgcgaagacc tggttccctt
      601 ccttcccgta cccagggtag aacacggcga gggagactac cgtcttgcgt gggagcggcc
      661 ggacaccata gggaatgtgc acgcgttctg cggcaacgtc ggcgtcatga tccgggcgta
      721 ctgctacatc cggatgatgg gcggcgaagg gctcgaacgc gtctcgcgca cggccgtcat
      781 caacgccaat tacctgtatg cgctggtgaa agaccttttc gatacacctt accagggccc
      841 ctacatgcac gaattcgtcg tctccggcga ccggcagaag gcacgcggcg cgagaacgct
      901 cgacattgcg aaacggctcc tcgacttcgg cgtgtacgcg ccgaccgtct actttcccct
      961 catcgtcagc gaagccatga tgatcgaacc cacggagaca gaatcgaaac aggccatcga
     1021 ggaatacgcg gcattcctcc gccagattgc gcgcgaagtg gacgaggagc cggagacggt
     1081 tctgaaggca cctcacacga cgcccatcgg ccgcgtggac gaggttcgcg ccgcgcgcca
     1141 gcctgatttg cggtatgggt ttcagggtgt gtgatcgccc tgtttcgtgg cagcgtctca
     1201 gtcgtggagg gtcacgcttc gtcgtgaccg atttctcaaa cccgttggac ccggcacagc
     1261 gggtccctcc atgacaggtt tgcgggtgtg gagggtcacg cttcgtcgtg accccatagc
     1321 caccacgagg ctcaatgcat atcaggaaat cacccgtcca catgccactc gttgaacggc
     1381 gtttttctcc ggcggtcgtc tacgttacgg tttgtacgaa ggacagaaag gcaattttgc
     1441 atcgcccgga gatcaacacc ctcctcggcg aagcatggca gtcggcggag ggctggctgg
     1501 tcgggcgcta tatcatcatg cccgatcatc tgcacctctt ttgtgcgccg gcgacgctgg
     1561 aataccccga actggcgaag tgggtgaagt gctggaaggc attcgtttcg ctgaactggc
     1621 cggtccgtgg agagcaaccg atctggcaac gctcgttctg ggatacccag ctacggagcg
     1681 ctgaggacta cgaaaacaaa caggattaca tggtgcacaa ccccgtccgc gcaggacttt
     1741 gcgccacgcc tggaaattgg cccttccaag gggaattgaa cgtgttatat tggtgaaacc
     1801 gggacccgac gaagcgggtc cctccaaggt gtcggcaacc ctggctctgc cttcatccgt
     1861 catgatggac atgggtactc tgtggggggg caagcttcgt cgtgaccccg cgacattgac
     1921 tcaagctacc gacaaatagg acccgacaaa gcgggtccct ccacccggga aatcggtatg
     1981 tatccggcct ataagaccca tctgttctcc agcgaactct tccgccgtgc ggaggaagcc
     2041 cgccggttcc ttgccccctg ccgcgtgtgc ccccgcgcct gcggcgtaga ccgactggcg
     2101 ggaaaaaccg gggtgtgcgg aatcggggcg aaggcaacgg tggccagcgc gctcgcccac
     2161 cacggcgagg agcccgggct cagcggcgcg cgcggcgccg gcaccatttt cttcggcgga
     2221 tgcaacctgc gctgtatctt ctgccagaac caccagatca gccagaccag tcattcgccg
     2281 gaatatggcc ctgaggagat cgccgcgatg atggtgcggc tgcagggcga gggctgccac
     2341 aacatcgacc tcgtctcccc ctcgcatgtg gcgccgcagg tacttgaggc gattgcccgc
     2401 gccgccgagc aggggctttc gatcccgatt ctctacaact ccaacgggta cgacagcctt
     2461 gatatgctcc gcctgttcga cggcgtggcc gatatctacc tgcccgacct gaaatacggc
     2521 gatgactcga caggaacgtg ggcaagcagc gtcgataact acgtcgaaca cgcgcacgcc
     2581 gcgctgaagg agatgtaccg gcaggtcggg gatgtggagt ttgacaacaa cggc
//
LOCUS       DAOTNG010000382         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30439_length_2632_cov_3.722934, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000382 DAOTNG010000000
VERSION     DAOTNG010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30439_length_2632_cov_3.722934"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..2344)
                     /locus_tag="P5179_13480"
     CDS             complement(179..2344)
                     /locus_tag="P5179_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96274.1"
                     /translation="MQEAVSAAPELPQSRVGGPWALDAKITRGSYLFNFGDMSEEKAD
                     DWIALAQSLGITQIDFHGGNSFRFGDCRPNPVTYPNGFASLKAVIDKLHAAGISAGLH
                     TYAFFIDKTCPWVTPVPDSRLAKDRILTLAAPIAADADTITVKEDTSGMSAITGFFVR
                     NSNTLHIGDELITYTGVSKEAPYGFTGCTRGACGTAPAAHPAGAAVGHLKECFGLFVP
                     DPETTLFEEVAAKATEAYNTCGFDMSYLDALDGEDILGGAEWAWHYGSRYVFEVAKRL
                     KKPALFEMSTFHHHLWYVRARIGAWDHPGRSHKKFIDLHVASNEQGRRMFLPAHLGWW
                     AVKTWHGHQTEPTFSDDIEYLCCKAVGTDTGFSVMGINPENRDKPVFQRLGAIMRRYE
                     ALRHSGTVPDAVKARLKEPGAEFTLVDTADGKSAFLPTQYDKHRVEGADGWSDSWTVN
                     NPYAAQPVRLRIEALLSAEPYDSPNAITIADFTDPARFSDRAAYDGITLDLAPAEGGA
                     LFSAHNSNPPKPSAKKELRYSPTEHGVRVTQKADPSWAKAGATFDPPLNIDKHEALGL
                     WIEGDGKGEVLNLQLKCPEHLVTGIGDHYVPITFTGRRFVTLIEPEGARADEYSWPYD
                     GNVYALYREGIDYTQIRSVSLWYNAIPAGESVSCRLGPIKALPMRPVTLRNPSVIMGG
                     QEITFPVGMKSGWYIEYAAGEPCILYDDQGTPVAEVTPR"
BASE COUNT          454 a          776 c          884 g          518 t
ORIGIN      
        1 cggacaccga agcaacgccg cgttgcaggt ccgcccctcg ttgtgctcgc acaattcctt
       61 gctaccctat cgcctcgcca cgagtgatca cggatacatg cgcgcgggcg ggaaggtgct
      121 cgtcggtttc gcagacgaag cgcacggagt tttccccgtg ttggagaaca ggggcggctc
      181 agcgcggcgt cacctcggcg accggggtgc cctgatcgtc gtagaggatg cacggctcac
      241 ctgcggcgta ctcgatgtac cacccacttt tcatgcccac cgggaacgtg atttcctgac
      301 cgcccattat cacggacggg ttgcgcagtg tcaccggccg catcgggagg gccttgatgg
      361 gaccgagccg gcacgacacc gattcccccg cgggtatggc gttgtaccag aggctcaccg
      421 aacggatctg ggtgtagtcg ataccttcgc ggtaaagcgc gtacacgttc ccgtcgtacg
      481 gccatgaata ctcatccgcg cgtgctcctt cgggctcgat gagagtcacg aaccgccgcc
      541 cggtgaaggt aatgggaacg tagtggtcgc cgatccccgt cacaaggtgt tccgggcact
      601 tcaactgcag gttcagcacc tcgccctttc catcgccctc gatccagagg ccgagcgctt
      661 cgtgcttgtc gatgttgagc ggcgggtcga acgtcgcgcc ggcctttgcc catgagggat
      721 cggccttctg cgtcacccga acgccgtgct ccgtcggtga gtaacgaagc tctttttttg
      781 cggagggctt cggcgggttg gaattgtgcg cggagaacag ggctccgcct tccgccggtg
      841 cgagatctag ggtaatgccg tcataggccg cgcgatcgga gaagcgggcc ggatcggtga
      901 aatccgcaat ggttatggcg ttgggcgaat cgtacggctc ggcggagagc agtgcttcga
      961 tgcgaaggcg caccggctgc gcggcgtacg ggttgtttac ggtccaggag tcgctccagc
     1021 cgtccgcacc ctccacacgg tgtttgtcgt actgcgtggg caggaacgcg gatttcccgt
     1081 cggctgtatc caccagcgtg aattcggcgc cgggctcctt gaggcgggct ttgacggcat
     1141 ccggaaccgt gccggaatgc cgcagagctt cgtaccgccg catgatggct ccaagacgct
     1201 ggaataccgg tttgtcgcgg ttttcggggt tgatccccat caccgagaag ccggtatccg
     1261 tgccgacggc cttgcaacag aggtattcga tgtcgtcgga gaaggtcggc tccgtctggt
     1321 gaccgtgcca cgtcttgacc gcccaccagc cgaggtgcgc ggggaggaac atacggcgac
     1381 cctgctcgtt cgaggcgacg tggagatcga tgaacttctt gtggctgcgt ccggggtggt
     1441 cccacgcacc aatgcgcgcg cggacgtacc acaggtggtg gtggaacgtg ctcatttcga
     1501 acagtgcggg cttcttgagg cgctttgcca cttcgaagac gtagcgcgag ccgtaatgcc
     1561 acgcccactc ggcgccgccc agaatgtcct cgccgtcgag cgcgtcgagg tagctcatgt
     1621 caaagccaca ggtgttgtac gcttcggtag ctttggcggc aacttcctcg aacagcgttg
     1681 tttcgggatc cgggacgaac agaccgaaac attccttcag gtggccaact gcggccccgg
     1741 ctggatgggc ggccggtgcc gttccgcagg caccgcgagt gcagccggta aacccgtagg
     1801 gtgcctcttt ggagaccccg gtgtaggtga tgagttcgtc gccaatgtgc agcgtgttgc
     1861 tgttgcggac gaaaaagccg gtgatggccg acatgccgga ggtgtcctct ttcacggtga
     1921 tcgtatcggc atcggcggcg atcggcgcgg ccagcgtcag gatgcggtcc ttggcgaggc
     1981 gggaatcggg caccggagtc acccatgggc aggttttgtc gatgaagaag gcgtaggtgt
     2041 ggagcccggc ggagatgccg gcggcatgga gcttatcgat cacggctttc aggctggcga
     2101 agccgttggg gtaggtgacg ggattgggcc ggcaatcgcc gaagcggaag ctgttgccgc
     2161 cgtggaagtc gatctgggtg atcccgagcg actgtgccag agcgatccaa tcgtcggcct
     2221 tttcctcgga catatcaccg aagttaaaca ggtatgagcc tcgcgtgatt ttggcatcca
     2281 gcgcccacgg tccaccgacg cgcgattgcg ggagctcggg ggctgcactg accgcttcct
     2341 gcataccttt gcgcagggta ttcatcgggg tggctacgag ggcaacggac gcaccggcaa
     2401 acccgaattt ggcgtaacac gtcgcgcaga gccgcgccat ggggccgggc agttcgcgca
     2461 cgttcgtttt cagattcagg gcgagcgcac acgcgccgaa cgggtcttcg agcgttcccg
     2521 cgaggcggag aggaatatgc gcgaaagtga attcttctac accgtcgcca tcaacggcga
     2581 cgacgcgcaa gacgatgtgg tgcttctccg cgtgtgccat gaggcgggct tt
//
LOCUS       DAOTNG010000383         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30531_length_2627_cov_4.241835, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000383 DAOTNG010000000
VERSION     DAOTNG010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30531_length_2627_cov_4.241835"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5179_13485"
     CDS             <1..441
                     /locus_tag="P5179_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRS96275.1"
                     /translation="ERYIPFIIEASSGVNRTLLAYLCDAYDVEQLENDTRTVLHLHPR
                     LAPVKAGIFPLVKRDGMPEKARQIERQLNPYFRVFYDESGAVGRRYRRMDEVGTPFCI
                     TVDSQTMQDDTVTLRDRDTMLQERYPVAGLVQVLGEKIASWKRP"
     gene            564..1604
                     /locus_tag="P5179_13490"
     CDS             564..1604
                     /locus_tag="P5179_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96276.1"
                     /translation="METLRVGIVGTGSIGMVHLDTYAKIPGYKVTAVCDVRETALAQA
                     RAKSGAAGFADYHDLVAQNDVDVVAVCTPNYLHAPVTIAALNAGKHVFVEKPMAYDVK
                     AAKEMIAARDKSGKLVQIGVCQRFRGDAQVLKSHIENGELGRIYFAKCGYLRRSGIPG
                     MGSWFTTRSQSGGGPILDLGVHALDLTMYLMDNFKPVGVMACKYAEFGPRGLGAGGWG
                     TAVPGGPFDVEDLAAALIKFENGATIFLEVSWAAHVGSGSFYSTLMGDKAGANLDPAT
                     IYTDEMGHPVDKKLTVPSVDAYHAEHVHFHDCIVNNRKPMPSVEQGLAVQAVLDGIAE
                     SADKGTSVSVSL"
     gene            1622..2581
                     /locus_tag="P5179_13495"
     CDS             1622..2581
                     /locus_tag="P5179_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRS96277.1"
                     /translation="MSVFTIGIDLGGTNVRAGVVTEDGQVISSCGHPTEVSKGQEHSI
                     RQIVRTAEEAVERAGISRQDISAVGIGSPGPLDPFNGLVLSPVNFPTWGTVPLAQILS
                     DAFHGVPTALDNDANVVAYGENWLGAGRDTANFLCITLGTGVGGGVVCEGKLLHGSDG
                     NAAEIGHISIDYNGPRCNCGGIGCLELYCSATAIARRTRAAMEAENPKTLLTGNEVTA
                     QTIADAANAGDEFARRMYDETGFFLGMGIVSAVALFNSEIVAVGGGMARAGDLIFEPA
                     RRTFHEFGFPSLRGRLRIEPMRLGDNAAILGAARLAMDGGIRR"
BASE COUNT          577 a          747 c          814 g          489 t
ORIGIN      
        1 gagcgatata tcccattcat catcgaggca tcttcaggcg taaaccgcac actgctcgcg
       61 tacctgtgcg acgcatacga cgtcgagcag ttggaaaacg acacacggac ggtgctccac
      121 ctgcatccgc gtctcgctcc ggtaaaagcc ggcattttcc cgctggtcaa gcgcgacgga
      181 atgccggaaa aggcgcgtca gatcgaacgg caattgaacc cctatttccg cgtgttctac
      241 gacgaaagcg gcgccgtcgg gcgcagatat cgccggatgg acgaggtggg cacgccgttc
      301 tgcatcaccg tcgattcaca aacgatgcag gacgataccg tcacgttgcg tgatcgcgac
      361 acgatgcttc aggagcgata cccggtcgcg ggtctggttc aggtactcgg ggagaaaatc
      421 gcgagctgga aacgtccatg atcggctcga atccggcaaa ggcaattgcg acaaactggc
      481 tccgtccgta gctttacaaa tgatttgttg ttctgagaac atgtgaataa ccgtcataac
      541 tatcgacaca ggggagaacg aagatggaaa cgttacgcgt gggaattgtg ggaaccggtt
      601 caatcgggat ggtccatctg gacacgtacg caaagatccc ggggtacaaa gttacggcgg
      661 tgtgtgatgt ccgcgagacc gcactcgccc aagccagggc caaatctggc gccgccggtt
      721 ttgcggatta ccatgacctg gtagcgcaga atgacgtaga cgtcgtcgcc gtgtgtacgc
      781 ccaactacct ccacgcaccg gtgacaattg ccgccctcaa tgccggcaaa cacgtgtttg
      841 tcgagaaacc gatggcatac gatgtcaaag ccgcgaagga gatgatcgcc gcgcgggata
      901 aatccggaaa gttggtccag atcggcgtct gtcaacgctt ccgcggcgac gcccaggttc
      961 tcaaatcgca cattgaaaac ggcgaactcg gacgcattta cttcgccaaa tgcggttacc
     1021 tccggcgcag cggcatcccc ggcatgggaa gctggttcac cacccgcagc cagagcggcg
     1081 gcggacccat tctcgacctc ggcgttcatg cacttgacct gacgatgtat ctcatggata
     1141 acttcaagcc ggtcggcgtc atggcgtgca aatatgccga gttcggcccg agaggtctgg
     1201 gtgccggtgg ctggggcacg gcagtcccgg gtggcccgtt cgatgtcgaa gacctcgcag
     1261 ccgcgctcat caaattcgag aacggggcga cgatcttcct cgaggtaagc tgggctgcac
     1321 acgtcggttc gggaagcttc tattcaaccc tcatgggcga caaagctgga gcaaatctcg
     1381 atccagccac gatctacacc gacgaaatgg gccatcccgt ggacaagaaa ctcacggttc
     1441 catctgtaga cgcctaccac gcggagcacg ttcacttcca cgattgtatc gtaaacaaca
     1501 gaaagccgat gccgagcgtc gaacaggggt tggctgttca ggctgtgctc gacggtatcg
     1561 ctgaatccgc ggacaaaggc acgtcagttt ctgtttcgct ctaaccgcca ccgggagtac
     1621 catgtcagtg ttcactatcg gtatagatct cggtggcacg aatgtccgcg ccggagtggt
     1681 gacggaagac ggacaggtta tctcgagttg cggccacccg acagaggtca gcaagggtca
     1741 ggaacactcc atacgccaga tcgtacgcac ggcggaagag gcagtagaac gcgcagggat
     1801 atccaggcag gatatctccg ccgtcgggat aggatcaccg gggccgttgg atccattcaa
     1861 cgggctggtg ctttcgcctg tcaatttccc gacgtggggc acggtcccgc tcgcgcagat
     1921 actgagcgac gcatttcacg gcgttccgac ggcgctcgac aacgacgcaa acgtggtggc
     1981 gtacggagag aattggctgg gggcgggacg cgacacggcc aatttcctct gcatcacgct
     2041 gggaacgggt gtcggcgggg gagtcgtgtg cgaagggaag ttgcttcacg ggtcggacgg
     2101 taacgccgcg gaaattggac atatcagtat cgactacaac ggtccccggt gtaactgcgg
     2161 cggaatcgga tgtctggagc tgtactgcag cgccaccgcc atcgcgcgac gaactcgcgc
     2221 cgcgatggag gcagaaaacc cgaaaacgtt gctcacggga aacgaagtaa ccgcgcagac
     2281 catcgcggac gccgccaacg cgggcgacga attcgccaga cgaatgtacg atgaaaccgg
     2341 atttttcctc ggaatgggca tcgtaagtgc ggtcgccctt ttcaacagcg aaatcgtcgc
     2401 ggtcggcggg ggcatggccc gtgcggggga cctgattttc gaaccggcgc gtcggacctt
     2461 ccacgagttc ggattcccgt cgctgagggg gcggctgcgc atcgagccga tgcgcctcgg
     2521 ggataacgct gccatcctcg gtgcggcgcg gctggctatg gatggaggga ttcgccgctg
     2581 aaaagacggg cgatagtctg gtacgcggca cttgcgctct cgttcgc
//
LOCUS       DAOTNG010000384         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30640_length_2619_cov_5.173557, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000384 DAOTNG010000000
VERSION     DAOTNG010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30640_length_2619_cov_5.173557"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="P5179_13500"
     CDS             complement(<1..522)
                     /locus_tag="P5179_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96278.1"
                     /translation="MPEFSISTQFVRYTVGTDAMNRAFTDVNTDTNYAIPAPCASIIK
                     GGVEFRATEAWEADGLLHLAFGGSGVKARLMAHAEKHHIVLRVVAVDGEGVEEFTFAH
                     IPLRLAGTLDDPFGASALALNLKTNVRELPGPMAQLCATCYAKFGFAGASVALVATPM
                     DTLRKGMQEAVSAA"
     gene            950..>2619
                     /locus_tag="P5179_13505"
     CDS             950..>2619
                     /locus_tag="P5179_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HRS96279.1"
                     /translation="MASSDSLLITPYGGTLVDLVIPSEEREHLAKYAGGLPSIQLTER
                     QLCDLELLATGAFSPLDRFMGAEDHARVLDEMRLSSGHLFPIPVTLSVDPSDAIHLDS
                     DIALRDAKNDLLAVMTVEEAYEWDRDEVSRKVFGTLDEKHPLVAEMRRWGKVNISGRL
                     RVIRLPRRYDFRELRLTPAQTRARLEAYGHANVVAFQTRNPLHRAHEELTKRAAESVD
                     GVLLLHPVVGMTKPGDVDHYTRVRTYKALAEHYYDPDRILLSLLPLAMRLAGPREAVW
                     HAIIRRNHGANHLIVGRDHAGPGNDSTGKPFYGPYDAQDLVQRHEKELGVKALPFSML
                     VYLPDEDRYEEDSKVPKNARTASISGTQVRDEYLNKGRALPSWFTRPEVAQILAESYP
                     PRDRQGFCVWFTGLSGAGKSTIADILAVRLLELGRQATVLDGDVVRTHLSKGLGFSKE
                     DRDANIRRIGFVASEIVRHGGCVIAAAVSPYRATRDEVRRMVGSDQFIEVFVNTPLET
                     CEQRDVKGMYAKARRGEIKGFTGIDDPYEPPVNPEVMLDTVACTPEMNT"
BASE COUNT          539 a          753 c          820 g          507 t
ORIGIN      
        1 ggcggcactg acggcttcct gcatgccttt gcgcagggta tccatcgggg tcgcaacgag
       61 ggcaacggac gcaccggcga aaccgaattt ggcgtaacag gtcgcgcaga gctgcgccat
      121 ggggccgggc agttcgcgca cgttcgtttt cagattcaga gcgagcgcac tggcgccaaa
      181 tggatcgtcg agtgtccctg caaggcgcaa cgggatgtgc gcgaaggtga attcttcgac
      241 accttcgccg tcgacggcaa cgacacgcag gacgatgtgg tgcttctccg cgtgtgccat
      301 gaggcgggct ttcacccccg aaccaccaaa tgcgaggtgc aacaggccat cggcctccca
      361 cgcttcggtc gcccggaact cgacgccacc cttgatgatg ctggcacatg gcgcggggat
      421 ggcgtaattc gtatcggtat ttacatccgt gaaggcccgg ttcattgcgt ctgtaccgac
      481 ggtgtagcgg acaaactgcg tggaaatgga gaattctggc atagaaggat catccctcaa
      541 tggcgggtgg aacagcgtgc gcgctgtttc gaataacgcc cggaggagga ggaagagcaa
      601 gcgcgggggt tagatggctc cgggttgccc ttctggtgcg gtgtcgcgta tcttaataac
      661 gttcgaagcc agagtctttc gccaattcca cgccgactac gcgttttccg atcatccatg
      721 caatactcgc caggcccctc ttttgcctca ggaacgcccg tacccggcac tatcatctgg
      781 ttctggcctc accttctatg agaacaaaca ggcgggtttc cccgatttct ggaacacgtg
      841 ggggatatcg cggcggttga gccgcgtttt gccctcgagt aataaggata ccctctcagt
      901 tactgcctga gcatactgcg gacgcaccct cacggagact actgaattga tggcttcaag
      961 tgattccctg ctcatcacgc cgtacggcgg cacactcgtg gaccttgtaa tcccctcgga
     1021 ggagagggag cacctcgcga aatacgccgg gggccttcca tccattcaac tgactgaacg
     1081 gcaactgtgc gacctggaat tgctggcgac cggcgcattc tcgccgcttg atcggttcat
     1141 gggcgcagaa gaccatgcgc gggttctcga cgaaatgcgg ctctcttcgg gacacctgtt
     1201 ccccatcccg gtgacgcttt ctgtcgatcc aagtgatgcc atccacctcg acagcgacat
     1261 cgccttgcgt gacgccaaga acgatctgct ggcggtaatg acggtagagg aagcgtacga
     1321 atgggaccgg gatgaggttt cgagaaaggt gttcggaacg ctggatgaga agcatccgct
     1381 ggtagccgaa atgcgccggt ggggcaaggt caacatatcg ggcagacttc gcgtgattcg
     1441 gcttccccgt cgctatgact tccgggaact gcggctcacc ccagcgcaga cgagggcccg
     1501 gctggaggcg tacggacatg ccaacgtcgt cgcgttccag acgcgaaatc cgctccacag
     1561 ggcgcacgag gaacttacga agcgcgcggc agaaagcgtg gacggggttc tcctgcttca
     1621 ccccgtggtc ggcatgacca agcccggcga cgtggaccat tacactcggg ttcgcacgta
     1681 caaggcgctg gcggagcact attacgatcc ggacagaatt ctcctttcgc ttctacctct
     1741 cgcaatgcgt ctcgcggggc cgagggaagc cgtctggcac gcaatcatcc gcagaaacca
     1801 tggcgcgaat catcttatcg tgggacgcga ccacgccgga cccggcaacg actcgacggg
     1861 caagcccttt tacggaccgt atgacgcgca ggatctcgtc cagcggcacg agaaggagct
     1921 tggcgtcaaa gcgcttccct tcagcatgct ggtgtatctg cctgacgagg accggtacga
     1981 agaggattcc aaagttccga agaacgcccg cacggcgtcg atttcgggga ctcaggtccg
     2041 ggacgaatac ctgaacaagg ggcgcgcgct gccttcgtgg ttcacacgtc ccgaggtcgc
     2101 gcagattctt gcggagtcct atcctccccg ggaccggcag gggttctgtg tatggttcac
     2161 cggtttgagc ggggccggca aatcaaccat cgcggatatc ctcgcagtgc gtctgctgga
     2221 acttggaagg caggcaactg tactggacgg ggacgtcgtg cggacgcatc tctcgaaggg
     2281 tctcggattc agcaaggaag acagggatgc gaacattcga cgcatcggat tcgtcgcgtc
     2341 ggagattgtg cgccacgggg gatgtgttat cgccgcggcc gtgagcccgt accgggctac
     2401 acgcgacgag gtacggcgca tggttggaag cgaccagttc attgaagtat tcgtcaacac
     2461 gcctctggag acgtgtgagc agagggacgt gaaggggatg tacgcaaaag cgcggcgagg
     2521 tgagatcaag gggttcaccg gaattgacga tccgtacgag ccgccggtca atccggaggt
     2581 aatgttggac accgtcgcct gcacgccgga aatgaatac
//
LOCUS       DAOTNG010000385         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30657_length_2618_cov_5.211081, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000385 DAOTNG010000000
VERSION     DAOTNG010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30657_length_2618_cov_5.211081"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="P5179_13510"
     CDS             complement(<1..448)
                     /locus_tag="P5179_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96280.1"
                     /translation="MHGATARTRHNRLFAVDTSGMRANALFLVVALAFSWGLGAARES
                     NTTALAPIFVTPRDPVYEFLDRAETKGLLSNAELSMKPWTRLSIARALLEINRADSSV
                     NRLTAVDKALLRFFAEEYREEIRAISDGDPFKDLPSDPSQVFSDRVE"
     gene            complement(456..1379)
                     /locus_tag="P5179_13515"
     CDS             complement(456..1379)
                     /locus_tag="P5179_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS96281.1"
                     /translation="MRVLVTGGAGFIGSHIVDGLVERGHSVSVVDNLSSGRRENVNPA
                     ARLHVVDVTSNELGKVMEEERPEVVFHEAAQMDVRKSVADPRFDATVNILGGLNLYEE
                     CVRLKVRKVVFASTGGAIYGEQETYPAPETHPQQPLSPYGIAKLANEKYLYFYKSTYG
                     LEHVILRYANVYGPRQSPHGEAGVVAIFTSRLLSGQVPTINGTGEQTRDYVYVGDVAA
                     ANMAALDYGSSDVFNVGTGIETDVNELYALLRDAVGTSVEPVHGPGKPGEQMRSVVDW
                     QKAHRLLGWSPATPLPEGLKRTVEWFRQQRG"
     gene            complement(1528..>2618)
                     /locus_tag="P5179_13520"
     CDS             complement(1528..>2618)
                     /locus_tag="P5179_13520"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRS96282.1"
                     /translation="HALRDLRAQPFIIPAMGSHGGGTAEGQARVLETLGITEQTTGAP
                     VVSSMEVMPLGSTPEGIPVVIDANAARADHVLLINRVKPHTEFGGRYQSGLAKMLLIG
                     LGKHQGAIRYHQAAVEMPFETLVFSAAQVVLERLPVLGGLAVLENAHGQVAEVVAVQA
                     DNLLRDEPALLARATEYMATIPFDNIDLLIVDELGKDISGSGMDTNVIRRKHWAHTRI
                     GEIVEQPAPRRVFVRDLTEATEGNAAGIGLADFALTRVVNKVDWRKTRMNAITATRPR
                     GAMVPVLCENDAEAIEASLASAGVGDTAAARIVRIRNTACLNHMHVSDTLLPHAHAEA
                     SLTYGEEPFALAFDEDGMLVPDKLPRGS"
BASE COUNT          507 a          791 c          763 g          557 t
ORIGIN      
        1 gctccacacg gtcggagaaa acctgagaag ggtccgacgg caggtccttg aacggatcgc
       61 cgtccgaaat cgcgcggatc tcctcccggt attcctctgc gaaaaagcgc aggagcgcct
      121 tgtccactgc agtgagacga ttcacagaag aatcggcgcg gttgatttca aggagcgccc
      181 gtgcgattga aaggcgggtc cagggcttca tcgaaagctc agcgttactc aggaggccct
      241 ttgtctccgc gcggtcaagg aattcgtaca cggggtcacg cggcgtgacg aagatgggtg
      301 ccagggccgt cgtgttactc tcgcgtgcgg ccccaagtcc ccatgaaaac gcaagtgcca
      361 ccacgagaaa cagcgcgttc gctcgcattc cgcttgtgtc aacagcgaac aaccggttgt
      421 gacgggttcg ggcagtcgcg ccgtgcattc aacacctatc cgcgttgctg gcgaaaccat
      481 tcaacggttc gtttcaaacc ttctgggaga ggggtcgccg gcgaccatcc gaggagacgg
      541 tgcgctttct gccagtccac cacgctccgc atctgctcac ccggtttgcc aggaccatgg
      601 accggctcca cgctggtgcc aacagcgtcg cggaggagcg cgtagagctc attcacatcg
      661 gtttcgatgc ccgttccgac gttgaacacg tcgctgctgc cgtaatcgag tgcagccatg
      721 ttcgcagcgg caacgtctcc cacgtacacg taatctcgcg tctgttctcc agttccgttg
      781 attgtcggaa cctgccccga aagaagacgc gacgtgaata tcgcgacgac cccggcctcg
      841 ccatgggggc tctgtcgcgg tccgtacacg ttcgcgtatc tgaggatgac gtgttccagc
      901 ccgtacgtgc ttttgtaaaa gtacaggtat ttctcattgg cgagtttggc tattccgtac
      961 ggcgaaagcg gttgttgcgg gtgtgtctcg ggcgcggggt acgtctcctg ttcgccgtaa
     1021 attgcgccgc ctgtagaagc aaacaccacc tttcgaacct tcaatcggac gcattcctcg
     1081 tacagattga ggcctccgag gatgttcacc gtggcatcga aacgcggatc ggcgacggac
     1141 ttgcgaacgt ccatctgggc tgcctcgtgg aacaccactt cggggcgctc ctcctccatg
     1201 accttcccca gttcgttcga tgtcacatcg accacgtgaa gtcgggcagc cggattgacg
     1261 ttctcccttc tccctgacga aaggttatcc acgacgctga ccgagtgtcc ccgttcgacg
     1321 aggccgtcca cgatgtgaga accgatgaaa ccggccccac cggttaccag tacgcgcatg
     1381 atgttctcca cagaatgaat acggtgaaat gccaaatgac ccgggcgggt atcctcttgc
     1441 attttcgaaa gtaacgaatt gtccgattgc atgcattggc tggatggtct ctgcagcact
     1501 tttctgcgcg cggaagtcgg aattggatca agaccctcgt ggaagcttgt ccggcaccaa
     1561 catgccgtct tcatcgaacg caagcgcgaa tggctcttcc ccatacgtga gagacgcttc
     1621 ggcatgagca tgcggcagga gagtgtcgct gacatgcatg tggttcaagc acgcggtgtt
     1681 ccgaattcgg acgatacgcg cggcggcagt gtctccaact ccggcggagg ccagggaggc
     1741 ttcgattgct tccgcgtcgt tttcgcacag gacaggcacc attgctccac gcgggcgcgt
     1801 tgccgtaatg gcgttcatgc gcgttttgcg ccagtccacc ttgttgacga cgcgcgtaag
     1861 tgcgaagtct gcaaggccga tgcccgcagc gttcccctcg gtcgcctccg tcagatcacg
     1921 aacgaacaca cgacggggtg ctggttgctc tactatctcc ccgatgcggg tatgcgccca
     1981 gtgtttgcgc cgaatgacgt tggtgtccat cccggacccg ctgatatcct ttcccagctc
     2041 atcaacaatc agcaaatcga tgttgtcaaa cgggatcgtt gccatgtact ccgtcgctct
     2101 ggccagaagg gctggctcat cgcgcaaaag attgtcagcc tgtaccgcca caacctcagc
     2161 gacctgcccg tgtgcgtttt caagcacggc aagacctccg agaacaggca agcgctcgag
     2221 cacgacttga gctgctgaaa agactaaggt ttcaaacggc atctccacgg cggcttgatg
     2281 gtaccggatg gctccctggt gctttccaag acctatgagg agcatttttg caaggccgct
     2341 ctggtaccga cctccgaatt ccgtgtgggg cttgaccctg ttaatgagca gaacgtggtc
     2401 tgcccgtgcc gcgttcgcgt cgatgacgac ggggatacct tcgggcgtgg aacccagcgg
     2461 catgacctcc atggaggata ccacgggtgc ccctgttgtc tgctcggtga ttccaagcgt
     2521 ttccaggacc cgcgcttggc cttccgcggt ccccccgccg tggctgccca tcgcgggtat
     2581 gatgaagggc tgcgcgcgca agtcgcgaag cgcgtgga
//
LOCUS       DAOTNG010000386         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30671_length_2617_cov_4.210773, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000386 DAOTNG010000000
VERSION     DAOTNG010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_2617_cov_4.210773"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5179_13525"
     CDS             <1..395
                     /locus_tag="P5179_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRS96283.1"
                     /translation="TQRETSPRTRGGDPSVDRDVFDAARLLDLVDGDETAFREITTTF
                     RETTRDLVSRLKEANENKDVEAMRRVAHTLKGSAGSFFAGQLQRASARLEQVCTDGTF
                     EEIARNVATVVEAWNRLEDRMNAGNRAE"
     gene            586..1644
                     /locus_tag="P5179_13530"
     CDS             586..1644
                     /locus_tag="P5179_13530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRS96284.1"
                     /translation="MSAKQIVAVFMGGRASEHDISLKTGTVVMNSLDRTRYTVYPVQL
                     CRDGGWIIPAEPFSGSTPWSPFDEAAGAAPIEAALRELRRIRPDVAFIALHGRFGEDG
                     CVQGLLELLDIPYTGSDVMSSALAMDKVRSKQIVAYHRIKTPRWIVVQEQEWRLQRSE
                     IIARVGREFGFPCVAKVPEEGSSFGMGIPQNETELEKVMDESIVARGHMMVEEYISGT
                     EITCAVLGGFPGEAPIAFPLTEIIPKNSAYFDFEAKYTPGASEEITPARLDPEITALA
                     QDIGVRSHIALGCGGMSRTDMIVRDREVFYLETNTIPGMTETSLYPQAARAMGMDFRD
                     LLTRQVELAIAYHRQKRA"
     gene            complement(1662..2432)
                     /locus_tag="P5179_13535"
     CDS             complement(1662..2432)
                     /locus_tag="P5179_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRS96285.1"
                     /translation="MAGEEGSLATDEHALLREALKLRETMVREIMTPRVQMVTLARHD
                     SLETAKSLCASSGFSRIPVTGADENEIVGVLFAKDLLLTYESGVGPTAVVVSDLMRPP
                     VFVPEVISVSRLLDTFRRHNTHLLIVVGEYGDVTGLVTMEDLLEKLVGAIEDEFDREE
                     PEIKALSERRFLVAATAQPDSIARALGVVLPEGEYETVAGYLIDRLGKIPPPGTAHRL
                     EDWLILVYDADDRRVRSVTFQYLGMPPLSANKRPEVAL"
BASE COUNT          560 a          746 c          795 g          516 t
ORIGIN      
        1 ggactcaacg agaaacgtcg ccacgcacaa gaggcgggga cccgtcggtt gacagagacg
       61 ttttcgacgc agcccgcctt ctggatcttg ttgatggtga tgaaacggcg ttccgggaga
      121 tcaccacaac gttccgcgaa acgacgcgcg acctcgtcag ccggttgaag gaagcgaacg
      181 aaaacaagga cgtcgaggcg atgcgccggg ttgcccacac cctgaaaggt tcggcgggaa
      241 gcttctttgc cggccagttg cagagggcaa gcgcgaggct ggaacaggtg tgcactgatg
      301 gcacgttcga ggaaatagcg cggaacgtcg cgaccgtggt agaggcttgg aatcgcctgg
      361 aagaccggat gaatgccggc aaccgggcag aatagaaacc cgaccgggga gggaggaaac
      421 cccggtcggg attgggtcgt acccgaaggg gaagtcgaga acgacctaag caggttgtat
      481 ctactctcta taacttccga cctttcgatt ggttccttgc agtagttgcg gcgcttcagt
      541 gcggcgtcgg cgccatgatg cgtaattcga agagagggcg tctccttgtc ggcaaaacag
      601 atcgtcgcgg tgtttatggg tggtcgcgca tccgaacacg acatttcgtt gaagacgggc
      661 acggtggtca tgaactcgtt ggatcgtacg cggtacacgg tctaccctgt tcagttgtgc
      721 cgtgatgggg ggtggattat tccggcggag ccgttttccg gaagtacgcc ttggagcccg
      781 ttcgatgaag ccgcgggggc ggcgccgatc gaggcggctc tccgtgaact gaggcgtatc
      841 cgcccggacg tggcattcat tgcgctccac gggcggttcg gcgaggatgg atgcgttcag
      901 gggcttctcg aactgctgga tattccgtac acggggtccg acgtgatgtc atccgcactc
      961 gccatggata aggtcagatc caagcagatt gtcgcgtacc accggatcaa gacgcccagg
     1021 tggatcgtgg tgcaagaaca ggagtggagg ctgcaacgtt ccgagatcat tgcgcgcgtt
     1081 ggccgtgaat tcgggttccc ctgcgtggcg aaagtgcctg aagaaggctc aagcttcgga
     1141 atggggatac cgcagaatga gactgagctc gagaaggtca tggacgagag catcgttgct
     1201 cgaggacaca tgatggtgga ggagtacatc tcgggaaccg agattacgtg tgctgttctg
     1261 ggcggattcc cgggtgaggc ccccatcgct ttcccattga cggagatcat ccccaaaaac
     1321 tcggcatact tcgatttcga agcgaaatac acgcccgggg cttcagagga aatcacgccc
     1381 gcgaggctgg atccagaaat cacggccctc gctcaggata tcggtgttcg gtcccacatc
     1441 gcccttggct gcggggggat gtcccgaacc gatatgatag tgcgcgaccg ggaagtattt
     1501 tacctcgaga caaacacgat tcccggtatg acagaaacct cgttgtatcc gcaggcagcc
     1561 cgcgcgatgg gaatggattt ccgtgacctc cttacccggc aggtggaatt ggccatcgcg
     1621 taccaccggc aaaagagggc gtgatcgtcg tgtgcatcca atcagagggc tacttctggc
     1681 cgtttgttgg cagagagcgg aggcatccca agatactgga aggtgactga tcgaacacgt
     1741 cgatcgtcag catcatacac caggatcaac cagtcctcaa gacgatgtgc tgttcctggc
     1801 ggggggatct tgccgagtct gtctatcagg tatcccgcga ctgtttcata ctcaccttcc
     1861 ggcagcacaa caccgagcgc acgggcgatt gaatccggtt gcgcggtcgc ggccacgagg
     1921 aaacgtcgct cgctcagggc tttgatttcc ggctcttccc tgtcaaactc gtcttcgatg
     1981 gcgccaacca gcttctcgag cagatcctcc atcgtaacga gtccggtcac atcgccatac
     2041 tcgcccacaa cgatcagcag atgcgtgttg tggcgtcgaa acgtatccag caatcgactg
     2101 actgagatca cttccggaac aaaaaccggc ggacgcatca ggtcgctgac cacaaccgcg
     2161 gtcggcccga cgcccgattc atacgtcaac agcaggtctt tcgcgaagag gacgcccaca
     2221 atctcgtttt catctgcacc tgtgactgga atacgggaga aacctgatga cgcacacagg
     2281 cttttcgctg tttccaggga gtcgtgccgg gcgagcgtga ccatctgaac gcgcggggtc
     2341 attatctcgc gcaccatagt ctcccgcagc ttcagcgctt cgcgcaatag cgcgtgctcg
     2401 tccgtcgcga gagaaccttc ctcaccggcc atggctaccg cggcttccag ctccgaccgc
     2461 actggagaat gccgcggttt tccggccatg acccagcgtg cgaccccgcg cgtgattcca
     2521 ccgaatatcc acaccaccgg gcacacactg tccctccacg acactccgcc gagcaccacc
     2581 gcgcgacatg tggattcaga atggctttac ccaagtt
//
LOCUS       DAOTNG010000387         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30857_length_2606_cov_2.950608, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000387 DAOTNG010000000
VERSION     DAOTNG010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30857_length_2606_cov_2.950608"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5179_13540"
     CDS             <1..965
                     /locus_tag="P5179_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96286.1"
                     /translation="VIPGWRDTSSVFFFDKRRQKYVWYGRPEAYAAPSMHANRLLAYR
                     ESEDLTHWTPDRTVLDTDDADADPHHLLDERALHGGQQTIATAEQRARVWAELTEGQT
                     PDTDRPLVRGRNRQWYGITAFPYADLYAGIVWMYDLPTGSMWTELLHSYDGVDWRREA
                     IRVPFLEAIPGTCMCTMASPPVDLAEETRLYYSVSNQSHHRVPNPSVKKGIRIAALRR
                     DRWVGYVAGDVQGELLTQVMEKPRDLTLNAQTEGDGWVRVEVDDADGRVIDGFSADEC
                     VPFSGDDLGWRPTWKSGKGVDLLAGQMVRLRISARRASLFALHT"
     gene            1028..2311
                     /locus_tag="P5179_13545"
     CDS             1028..2311
                     /locus_tag="P5179_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS96287.1"
                     /translation="MILSCFIPKATRRLLLAFAVALGSHISESWAQAAPVERVVQSVP
                     QGGEDAVDWVNRRHTFTMNGQPYGFTALPIIFYTPNSGFHYGGWAEITNYAIRPYRYR
                     INTQWWLSTLGKRNHHIRMDIPELWGSRFNLRVVTQDLKDIGASYFGIGNGTRIDKEA
                     ERRESNFYRYKLEQQRTALDLEAELWGPFTVFSGVRFQRSSPSRVDETKSDYLVFSDP
                     KPLGLAAGWSNPLSAGLLVDTRDDQEFPTSGFLAELSGQEAFSWMNPDYVYRRVTIAY
                     TQYRTLAVHNLRPRTILVLRGLFENLEGEVPFYELTEVGGSVRGFDLAGNTMMRGYES
                     RRFADKQKILLSAELRRVLKDQTLWGHFFQSQGIVFADAGRVANKLKELNPVGLHPSG
                     GVGARLTWNAQLSLRTDFALSPEGHKILISFGSLF"
     gene            complement(2362..>2606)
                     /locus_tag="P5179_13550"
     CDS             complement(2362..>2606)
                     /locus_tag="P5179_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS96288.1"
                     /translation="MNIEGLAAKYKGKITFWGEIDRQYLMPFGTTEEIRAGVRRVRRA
                     LDDGRGGVIAQCEWGVRDPIENVLAVFDEWKKPRLR"
BASE COUNT          535 a          751 c          791 g          529 t
ORIGIN      
        1 cggtgatccc cggttggcgg gatacttcaa gcgtcttctt tttcgacaag aggaggcaaa
       61 aatacgtctg gtacgggcgg ccagaggctt atgcggcccc ttccatgcac gcgaaccgcc
      121 tgctggcgta ccgggaaagc gaggacctga cacactggac tccggacaga acagttctcg
      181 acacagatga tgcggatgcc gatcctcacc acctcctcga tgaaagagcg ttgcacggag
      241 gacaacagac gatcgccacc gcggaacaac gtgcccgggt gtgggcggaa ctcacggagg
      301 ggcagacgcc tgacaccgac cggcctctgg ttcgggggcg caaccggcaa tggtatggaa
      361 tcaccgcgtt cccgtacgcc gacctgtacg cggggattgt ctggatgtac gatttgccga
      421 ccggcagcat gtggacggaa cttctgcaca gctacgatgg cgtggactgg cgcagggaag
      481 cgattcgcgt gccattcctc gaagcgattc cgggaacgtg catgtgcacg atggcatccc
      541 cgcctgtgga ccttgccgag gaaacgaggc tgtactattc cgtgtcgaac cagagccatc
      601 accgggtccc aaatccctcg gtgaagaaag ggatccgcat cgcggcgctt cgtcgggacc
      661 ggtgggtcgg gtacgtggcg ggggatgttc agggggaact tctgacgcag gtgatggaaa
      721 aaccgcgcga cctgaccctc aacgcgcaga ctgagggaga tggctgggtg cgtgtggaag
      781 tggacgacgc ggatgggcgg gtgatagacg ggttttccgc ggacgagtgc gttccatttt
      841 ctggcgacga tctgggctgg cggccgacgt ggaagagcgg caaaggggtg gacttgttgg
      901 cggggcagat ggttcgtctg cgcatttccg cgcggcgcgc ttcgcttttc gcacttcaca
      961 cgtgacggga aaaatgagca ggaggaggct tggtcccgtt tgtcctttgc ccacgctgga
     1021 ggctgcggtg attctgtcgt gttttatccc gaaggcaacg cggcgccttc tgctggcgtt
     1081 cgccgttgct ttggggtcgc acatatccga aagctgggca caggccgcgc ccgtcgagcg
     1141 ggttgtgcaa agcgtaccac agggcggcga agacgcagtc gattgggtca atcggagaca
     1201 cactttcacg atgaacggac agccctacgg gttcaccgcg ttaccaatca tcttctacac
     1261 ccccaactcc ggtttccatt acggaggctg ggcggaaata acgaattacg caatccgtcc
     1321 gtaccggtac cgcatcaaca cgcagtggtg gttatccacg cttggcaagc gtaaccacca
     1381 tattcgcatg gatattccgg agctctgggg ttcgcgattc aacttgaggg ttgtcactca
     1441 ggacctgaag gatatcggcg caagctactt cggcattggc aatggcacga gaattgacaa
     1501 agaagctgaa cgcagggagt caaatttcta ccgctacaaa ttggaacaac agcggacggc
     1561 actcgatctc gaggctgagc tatgggggcc tttcacagtc ttttccggtg ttcgctttca
     1621 gcgcagctcc ccttcccgcg tcgatgagac caagagcgac tacctcgtgt tttccgaccc
     1681 caaacctctc ggactagccg cggggtggag caaccccctc agtgccggcc ttctggtcga
     1741 tacacgcgat gatcaggagt tcccgacaag cggctttctc gcggaactct ccggacagga
     1801 agcgttcagt tggatgaacc ctgactacgt gtatcggcgc gtcaccattg cctacacgca
     1861 gtatcgcacg ctagccgttc ataaccttcg tccacgaacg attctcgtcc tgcgagggct
     1921 attcgagaac ctcgaaggag aagtgccgtt ctacgaactt acggaggtcg gaggatctgt
     1981 ccgcgggttt gatcttgcag ggaatacgat gatgcgcgga tatgagtccc gtcggttcgc
     2041 cgacaaacag aaaatccttc tcagcgccga attgcgacgg gtgctgaaag accagaccct
     2101 ttgggggcac ttttttcagt cccaggggat tgtttttgcc gacgcgggcc gcgtggccaa
     2161 caaactcaag gagctcaatc ccgttggtct tcatccgagc ggcggcgtgg gagcacgctt
     2221 gacgtggaac gcccagctca gcctccggac cgatttcgcg ctttcacccg agggacacaa
     2281 aatcctgata tcgttcggaa gcctgttcta gcgcatctca aatcggtgca taccacaaaa
     2341 aaacagggcg cgaagcctag ctcatcgcag tcgcggcttt ttccactcgt caaaaaccgc
     2401 cagaacgttt tcaatcgggt ctcgcacgcc ccattcgcac tgggctatca cgccgccacg
     2461 accgtcgtcc agagcccggc ggacgcggcg caccccggcg cggatctcct cggtcgttcc
     2521 aaacggcatg aggtactggc ggtcgatttc cccccagaag gtgattttgc ctttgtattt
     2581 cgcggccagg ccctcgatgt tcatgc
//
LOCUS       DAOTNG010000388         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_30869_length_2605_cov_4.635294, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000388 DAOTNG010000000
VERSION     DAOTNG010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30869_length_2605_cov_4.635294"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5179_13555"
     CDS             <1..782
                     /locus_tag="P5179_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS96289.1"
                     /translation="RGKDMTDGGAHYHYHSTCAIGLPNVGDSLYAVDQLVFKEKSVSP
                     DELLEALKANWEGEEREKLRQKVIHRLAKYGNGNGDVDRWVERAAHHYCDHMATYTTP
                     HGGSFHVHLFSFVWHLDCGRATNATPDGRKAHDPLAYSVSPQQGRDVEGLTALMRSLM
                     TLPHEHAAGSSSAIIEIAPKLLDEVSLDKVLDVVQTAIDKGVGQMQFNVIDSATLRRA
                     QENPEAYSHIAVRVSGFSMRFCLLDKTMQDHIIARTKHEEM"
     gene            807..>2605
                     /locus_tag="P5179_13560"
     CDS             807..>2605
                     /locus_tag="P5179_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS96290.1"
                     /translation="MIQPILRTERIARLRKWQFDQRGGQVDREMLWHRYMAGHQSLPI
                     DARYAGVFAYLMDNLPVEINDDELIVGRIKRREMTPEEKAERERIAGSTFGDPLGDIL
                     KKLISPDAHESVAEGRHVAGAMTGHMTPDYPAVLRKGLNGIRREVRERLTRATTEKER
                     AFCHAALEVLSGAMRMARRYATHARELAATATPERREELLRIAEICRRVPAWPARTFH
                     EAAQTLWFIHLWIAQELGGGHGCFCPGRVDQYLYPYYEADVRAGRLSREDAYEILTCL
                     LLKFNEFDPYSTPQTLFVGGQHADGSDASNELSLLCLEVSRDLQMLHPALCVSWHRNL
                     NRDVMRRAVELMGTGIGFPAVFNDEVIVPGLMRDGASREEAVNYMAGSCVEISAIGCS
                     NPWVASGYVNAGKAFERAFQRVARNKNAGWDDLVREFKADLAEAMRFNYEITAAHDHA
                     WQECIRYPFLSCLVNDCIERATDITCGGARYNTTMPELVGLANVVDSLMAVKWACFEE
                     KAMTLPQLAEIVRKNWEGEEALRRVLGSKPPRFGNDMPEPDALWVELSEFWFDEVRKY
                     TNPRGGPYQPGFLCWVMHSVLGDKTGATPDGRKA"
BASE COUNT          535 a          785 c          842 g          443 t
ORIGIN      
        1 cgcgcgggaa ggatatgacc gacggcgggg cgcattacca ttaccattcc acctgcgcga
       61 taggcttgcc caatgtcggt gactccctgt atgcggtgga ccagctcgtc ttcaaggaga
      121 aaagcgtctc ccccgacgaa ttgctggaag cgctcaaggc gaactgggaa ggggaggaac
      181 gggagaaact ccggcagaag gtgatccaca ggctcgcgaa gtacggaaac gggaacggcg
      241 acgtggaccg ctgggtggaa cgtgcggccc accactactg cgatcatatg gccacgtaca
      301 ccacgccgca tggcgggtcg ttccatgtgc acctgttctc attcgtatgg catctcgact
      361 gcgggcgcgc caccaatgcc accccggacg ggcgcaaggc gcacgatccg ctggcgtaca
      421 gcgtctcccc gcaacagggg cgcgacgtcg aggggctgac ggcgctgatg cggtcgttga
      481 tgacgttgcc ccacgaacat gctgcgggtt cgtcatccgc gatcatcgag atcgccccga
      541 aactgctgga cgaagtgtcg ctggacaagg tccttgatgt cgttcaaacg gcgattgaca
      601 aaggcgtggg gcagatgcag ttcaacgtga tcgacagcgc caccctgcgc cgcgcacagg
      661 agaaccccga ggcctactcg catatcgcgg tccgggtgtc gggtttcagc atgcgcttct
      721 gcctgctgga caagacgatg caggaccaca tcatcgcacg cacaaaacac gaagagatgt
      781 gaacggacgt ctccggagga attggagtga tacagccgat tctgcgtaca gaacgcatcg
      841 cgcggctccg aaagtggcag ttcgatcagc gcggggggca ggtggatcgc gagatgctct
      901 ggcaccgcta catggccggg catcagagcc tcccgataga cgcacggtat gccggcgtgt
      961 ttgcgtacct catggataac ctccccgtcg agatcaacga cgacgaactg atcgtcggcc
     1021 ggatcaaacg gcgcgagatg acgcccgagg aaaaagcgga acgtgaacgt atcgcaggta
     1081 gtacctttgg tgatccattg ggcgacatat tgaagaaact catttcgccc gatgcgcatg
     1141 agtcggtcgc cgaaggccgc catgtggcag gcgcgatgac cggtcacatg acgcccgatt
     1201 acccggctgt gctcaggaag ggcctcaacg gaatccgacg cgaggttcgc gaacggctga
     1261 cacgcgcgac aactgagaag gaacgagcgt tttgccacgc cgcgctggag gtgttgtccg
     1321 gcgcaatgcg gatggcccgg cgctacgcca cgcacgcccg cgaactggcg gccaccgcaa
     1381 cccccgaacg gcgcgaggaa ctgctccgca tcgccgaaat ttgtcgccgc gtgcctgcct
     1441 ggccggcgcg gaccttccac gaagccgcgc agacgctctg gttcatccac ctctggatcg
     1501 ctcaagagct cggcggcggg cacggctgtt tctgccccgg acgggtagac cagtacctgt
     1561 acccgtatta cgaggcggat gtgcgtgccg ggcggctctc ccgggaggac gcgtacgaaa
     1621 tcctcacctg cctgctgctc aagttcaacg agttcgaccc ctattcaacg ccgcagactc
     1681 tctttgtcgg cgggcagcac gcggatggct ccgacgcatc caacgaactc agcctgctgt
     1741 gcctcgaggt gtcgcgcgat ctccagatgc ttcatcctgc gctctgcgta agctggcaca
     1801 ggaacctgaa ccgcgatgtc atgcgccggg cggtggaact tatgggcacg gggatcggat
     1861 ttccggcagt gttcaacgac gaagtgatcg tgccggggct gatgcgggac ggcgcaagcc
     1921 gcgaagaagc cgtcaactac atggccggtt cctgcgtgga aatctcggcg atcggatgct
     1981 ccaatccgtg ggtggcgtcc gggtatgtga acgccggaaa agcctttgaa cgagccttcc
     2041 agcgagtcgc acgcaacaaa aacgccgggt gggatgacct cgtgcgcgag ttcaaggccg
     2101 atctcgccga ggcgatgcgg ttcaattacg agatcaccgc ggcgcacgac cacgcctggc
     2161 aggagtgcat ccgctacccg ttcctgtcct gtcttgtgaa cgactgcatc gaacgggcga
     2221 ccgacatcac ctgtggcggc gcgcggtaca acacgaccat gcccgagctg gtcggcctcg
     2281 ccaacgtcgt tgacagcctg atggccgtca agtgggcgtg ctttgaggaa aaggcgatga
     2341 cgctgccgca actggcggaa atcgtgcgga aaaactggga aggcgaagag gcgctgcgtc
     2401 gcgtgctcgg ttcgaagccg ccgcggttcg gaaacgacat gccagaacca gacgcgctct
     2461 gggtggagct ttcggagttc tggttcgatg aagtgcggaa atacacgaat ccacgcggag
     2521 gcccgtatca acccggcttc ctctgctggg tgatgcattc cgtgctgggc gataaaaccg
     2581 gcgcgacgcc ggacggccgg aaagc
//
LOCUS       DAOTNG010000389         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_31078_length_2591_cov_4.749606, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000389 DAOTNG010000000
VERSION     DAOTNG010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31078_length_2591_cov_4.749606"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1095)
                     /locus_tag="P5179_13565"
     CDS             complement(163..1095)
                     /locus_tag="P5179_13565"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS96291.1"
                     /translation="MKVLLIGGTRFLGLAIANALHRHGHKAVVAHRGHTKAALPETAR
                     EIHVDVADQKSLENCLLAEHYDAVVDTILGAKTLEWMVTTLNGRIRQFVHCGSTGVYA
                     PMKRIPATEEDPCEALPQYGGFGAKLEQDEVLMRAHRQTGFPATVLRPTNIYGAGDIP
                     LDIWGARNPAFFRRLTRNQPVTLPNDGRALLQPGHVSELGEAFALALESPHSIGRIYN
                     ISSARAVELRVYLDILKGILKSSSPVDFASAELLLERYLPENKINEGGLRFVVEHMCV
                     DISRARAELGFDPQISLEEGLAENLQWMREKGLI"
     gene            complement(1092..1919)
                     /locus_tag="P5179_13570"
     CDS             complement(1092..1919)
                     /locus_tag="P5179_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRS96292.1"
                     /translation="MKWTEMTAPALADLSPDHVLLLPLGSCEQHGQHLPVFTDTFITT
                     AIADSVEKRIPHRVVQLPTLWLGASHHHRLFAGVLSLSESVYMTVLTGILECLADGPV
                     CAPGHAKRILLLNGHGGNVYPGQAALSEFAWKFRSRPDLIAAFASYWTLSDDAIKTVP
                     METPALTHSCEYETSLMLAIKGELVDMSKAKAGSLQWQHTRFTPDASRPSKVGVAAPF
                     HARSSNGALGHPELSSEEKGKQLLDVIVHDTIAFIEEFVRWDDLKDVRAAQSGEVAE"
     gene            complement(1930..>2591)
                     /gene="ricT"
                     /locus_tag="P5179_13575"
     CDS             complement(1930..>2591)
                     /gene="ricT"
                     /locus_tag="P5179_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRS96293.1"
                     /translation="KKKTVLRLAESHEISRWNALRTKEEEALRDFQQRISRRKMPMKP
                     VDVEYQLDGKKICFYFTADHRVDFRELVRELASVYRTRIELRQIGVRDEAKRLGGIGV
                     CGRELCCATFLAEFAPITSQMARDQNLSLNPSKLSGICGRLKCCLRFEQEFYRESHER
                     FPRLGTAFAANGHSGTVVKIDFLHETVTLRTEDQDEWTVSLKGTTATHSSRHPKNSNA
                     N"
BASE COUNT          502 a          736 c          776 g          577 t
ORIGIN      
        1 cgacaacccc tggcgtgtca tcaatgagat aggcgacgcc gcgcagatcg gcttcaatct
       61 cgggacgtag agccggaaaa ggggtgctcg agccccgttg caccttgcct gcccagggtg
      121 tgcgcggagt tccgcgcacg caggaaacgt gttcaaccgt agttagatca gtcccttttc
      181 gcgcatccac tgaaggttct ctgcaagccc ttcctccagc gaaatctgcg gatcgaatcc
      241 cagctccgcc cgcgcacgag agatgtccac gcacatgtgt tccaccacga accgcagacc
      301 gccttcgttg attttgttct ccggcaggta ccgttccaga agcaattcgg cggatgcgaa
      361 atcgacaggt gaggaactct tgaggattcc tttcagaatg tcgagataga cgcggagttc
      421 aacggcgcga gcgctcgata tgttgtaaat gcgtccgatc gagtgaggcg attccagcgc
      481 gagggcaaat gcctcgccga gttccgaaac gtgacccggt tggagaagcg cccgaccgtc
      541 attcggcaac gtgacgggct ggttccgcgt gaggcgtcgg aaaaatgccg gattccgtgc
      601 gccccagata tccagcggaa tgtcgcccgc accgtagatg ttggtaggac gaaggacggt
      661 tgcggggaac cccgtctgtc ggtgggcgcg catcaggact tcgtcctgtt ccagtttggc
      721 gccgaacccg ccgtactgcg gaagcgcctc gcaagggtct tcttccgttg caggtatgcg
      781 cttcatgggc gcgtacactc cggtagaccc gcagtgcaca aactggcgaa tccttccgtt
      841 gagggtcgtg accatccatt ccaacgtttt tgcgccgagt atcgtatcca caaccgcatc
      901 gtagtgctcg gccaggaggc agttctcgag cgatttctgg tccgccacgt caacgtggat
      961 ttcccgtgca gtctcgggca atgcggcttt cgtatgtccg cggtgggcta ccactgcctt
     1021 gtgcccgtgg cggtgcaagg cgttcgcaat cgcaaggccg aggaaacgag ttccaccgat
     1081 gaggagcact ttcattccgc aacctcccct gattgtgccg cgcggacgtc tttgagatcg
     1141 tcccagcgca cgaattcttc gatgaacgca attgtgtcgt ggacaataac gtccagcagt
     1201 tgttttccct tttcttccga cgaaagctcc gggtggccca acgcgccgtt ggagctccgt
     1261 gcgtgaaacg gtgcggctac gccgaccttt gatggtcggc tcgcgtccgg cgtgaaacgc
     1321 gtgtgctgcc attgcaggct gccggccttc gcctttgaca tatccacaag ttcgcccttg
     1381 atcgcaagca tcaacgaggt ttcgtactcg catgagtgtg tcagggcggg cgtttccatg
     1441 gggaccgtct tgatcgcgtc gtcggaaagg gtccagtaac ttgcgaatgc cgcgataagg
     1501 tcgggccggg aacggaattt ccatgcgaat tcactgagtg ctgcctgccc ggggtacaca
     1561 ttgccaccgt gcccgttaag aaggagtatc cgttttgcat gccccggagc gcacacaggc
     1621 ccgtcggcaa ggcattccag tatgccggta agcacggtca tgtacacgct ctcggaaaga
     1681 ctgaggacgc ccgcgaagag gcggtgatga tgtgaagcgc ccaaccaaag ggtggggagc
     1741 tgcacgacgc gatgtggaat ccgtttctcc acgctatcgg cgattgccgt cgtgatgaac
     1801 gtatcggtga agacgggcag gtgctgaccg tgctgttcac aggatccgag cggcaacaac
     1861 aggacatgat ccgggctcag gtctgccaga gcgggagccg tcatttcggt ccatttcatt
     1921 cgtgttacct caatttgcgt ttgagttctt tggatggcgt gaggagtggg tggccgttgt
     1981 tcctttgagg gaaaccgtcc attcgtcctg atcctcggtt cgcaacgtga cggtttcgtg
     2041 caggaaatcg attttcacga ccgttcctga gtggccgttg gcggcaaacg ctgttccgag
     2101 tcttgggaac cgttcgtggg actcccggta gaactcctgt tcaaaacgca ggcagcattt
     2161 gaggcgtccg cagatgcccg aaagtttgga aggattgagc gacaggttct gatctcgcgc
     2221 catctggctc gtgatagggg cgaactccgc gaggaatgtt gcgcaacaca gctcgcgacc
     2281 gcacacgccg atgccaccca ggcgcttcgc ttcatcgcgg acgccaatct gtcgcaattc
     2341 gatgcgtgtc cggtacacgg atgcaagttc gcggaccagt tctctgaaat cgacgcggtg
     2401 gtcggcggtg aaatagaagc agattttctt cccgtccagt tgatactcca catccacggg
     2461 tttcattggc attttgcggc gcgaaatcct ctgctgaaaa tcccgcaacg cctcttcctc
     2521 tttggttcgc agagcattcc agcgggagat ttcgtgagat tccgccaatc ggaggacggt
     2581 tttcttcttt g
//
LOCUS       DAOTNG010000390         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_31510_length_2564_cov_5.522120, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000390 DAOTNG010000000
VERSION     DAOTNG010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31510_length_2564_cov_5.522120"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="P5179_13580"
     CDS             complement(<1..564)
                     /locus_tag="P5179_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96294.1"
                     /translation="MEKLRVGVIGVGGMGTGHCEGLTKNVEETVLAAVCDIDPNVAKE
                     KAKTYGVKAFTSHRKLIKSGLVDAVTIATPHYFHSPIAVAAFEAGLHVLSEKPIAVTV
                     KAGDEMIAAAKASGLVFGVMFQQRTRPVSQAMKKIVSSGALGRIYRAMMVETHFRTQG
                     YYDSATWRATWKGEGGGVLLNQAPHGID"
     gene            complement(577..1332)
                     /locus_tag="P5179_13585"
     CDS             complement(577..1332)
                     /locus_tag="P5179_13585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS96295.1"
                     /translation="MATLSSFRWPNGARGAVSLSFDDGLRTHVENAIPTLNDYRFRGT
                     FYIPMVRGGAFDQRIDHWRAAASQGHEIGNHSMTHPCSANFGFSRRALDYLTLEDITE
                     EILDAKRRITEDIPDQKAHSFAYPCGETKVGSGKSHVSYVPVVAEHYVVGRGIGDFAN
                     DPVLCDLACTWSFMPTDPPAERLIGWADMARNMGRWSIFCFHGVGGEHFHLRMEAFRM
                     LLDHLAAHRDQIWTDTVINVGTWIRDHRERVCA"
     gene            1522..2547
                     /locus_tag="P5179_13590"
     CDS             1522..2547
                     /locus_tag="P5179_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96296.1"
                     /translation="MNHKERLLAAIDHRETDRTPIWLGCPHGDSHARLHKYFGVNSNL
                     ELQTAIDDDMRWFPIGWKHPEGKPGLDPTGGRPKKSHNQPGIFAETEDVGEVEKAYWP
                     DPQYADVAGARDAIDPWKHTHAIFSGVWAPFFHNVGDYFGMDNYFVKMHTNPSVVEAV
                     TERVVDFYWGVNEIAFSHLASDIDVMFFGNDFGTQLNTFVSPELFRRFILPGMKKLTD
                     QAHRFGLRVALHSCGSVYGVIPDLIGIGVEALHPIQGKARNMEAVRLKREFGNDLCFI
                     GGIDTQELLCNGTPQQVRDEVRRVIDIFGTGIVISPSHEAILPDVPPENVDAMFDEAR
                     TYHAKPR"
BASE COUNT          549 a          749 c          714 g          552 t
ORIGIN      
        1 gtcgataccg tgtggagcct ggttcagcag cactccgccg ccttcccctt tccacgttgc
       61 gcgccacgtg gcggaatcgt agtagccctg cgtcctgaag tgggtttcca ccatcatcgc
      121 tcgatagata cgtcccagcg cgccgcttga gacgattttc ttcatcgcct gactgaccgg
      181 acgcgtgcgc tgttgaaaca tgacaccgaa caccagcccg ctggctttcg ccgctgcaat
      241 catctcgtca cccgctttga cggtcactgc gatcggtttt tctgagagca cgtgcagacc
      301 cgcttcaaac gccgcaaccg caatggggga gtggaaatag tgcggagtcg cgatggtcac
      361 ggcatccacg aggccgcttt tgatgagctt cctgtggctg gtgaaggctt tcacgccgta
      421 cgtcttcgct ttctccttcg cgacgttcgg atcaatgtcg cagactgccg ccaagactgt
      481 ttcttcgacg ttcttggtca atccctcgca gtgtccggta cccataccgc cgacaccgat
      541 cacacctacc cggagtttct ccattttgca ttctccttat gcgcaaacgc gctcgcgatg
      601 gtcgcgaatc cacgttccca cgttgatgac ggtatccgtc cagatttgat cgcggtgcgc
      661 tgccagatga tcgagcagca ttcgaaaagc ttccatccgc aggtgaaaat gctccccacc
      721 aaccccgtgg aagcagaaaa tggaccacct gcccatgttc ctcgccatat ccgcccaccc
      781 gataagcctt tccgccggcg gatcggtcgg cataaaggac cacgtacagg cgagatcaca
      841 gaggacgggg tcgttggcga aatctccgat tccgcggccg acaacgtaat gctctgctac
      901 gacaggcaca tagctgacat ggctcttccc cgaccctacc ttcgtttcac cgcacgggta
      961 ggcaaacgaa tgtgccttct ggtccgggat atcctctgtt atccggcgtt ttgcatcaag
     1021 gatttcctcg gtgatgtctt ccagcgtgag gtagtccaga gctcgccggc tgaatccaaa
     1081 attcgcagaa cacgggtgtg tcatgctgtg gttaccgatt tcgtgcccct ggctcgcggc
     1141 cgctctccag tggtcaattc gttgatcgaa cgcccctccg cgcaccatcg gaatgtagaa
     1201 cgtgccgcgg aacctgtagt cgttcagcgt cggaatcgcg ttctccacat gggttctgag
     1261 gccgtcgtca aaggaaaggg agaccgcgcc tcgtgctccg ttgggccatc ggaaggaact
     1321 cagtgttgcc aaagggtgca ttcccggttt gatgaaacaa ggtgcgttga acccgtgaca
     1381 gaccgaaaga agagtatacg ccaccagacg gagcgctcca atcgcagcgc gtttcgcctt
     1441 acagactccc ttccgcattg gggactcgag ggcgatactt tggtacgcaa tggtctctac
     1501 cagatgccgg gaggctgaat aatgaaccac aaagagcgcc tgcttgcggc aattgatcac
     1561 agggagacgg acagaacacc gatttggctc ggatgtcctc acggagattc ccacgccagg
     1621 ctccacaagt atttcggagt caactcaaat cttgaactgc aaacagcgat tgacgatgac
     1681 atgaggtggt tcccgatcgg gtggaagcac cctgagggga aacccggcct ggatccaaca
     1741 ggagggagac ccaaaaagtc gcacaatcag ccggggatct tcgcggagac tgaggacgtg
     1801 ggcgaggtgg aaaaggcgta ctggcccgat ccgcagtatg ccgacgttgc cggcgcgcga
     1861 gacgccatcg atccgtggaa gcatacacac gcaatttttt cgggggtgtg ggcgcctttt
     1921 ttccacaacg tgggcgacta cttcggcatg gacaactact tcgtcaaaat gcataccaac
     1981 ccgtctgttg tcgaggcggt aaccgaacgc gtggtggatt tctactgggg cgtcaatgag
     2041 attgcgttct cgcatctggc gtcagacatt gatgtgatgt tttttggcaa cgatttcgga
     2101 acgcaactca acacattcgt aagccccgaa ttgttccgca ggttcatcct ccccggtatg
     2161 aagaagctga ccgatcaggc acaccgcttc gggttgcgag tggcgttaca ctcctgcggt
     2221 tccgtatacg gcgttattcc ggacctgatc gggatcggcg tggaggcact ccaccccatc
     2281 cagggcaaag ctcgcaatat ggaggcggtt cggctcaaac gcgaattcgg aaacgatctc
     2341 tgtttcattg gagggatcga cacgcaggag ctgttgtgca acggaacgcc tcaacaggtc
     2401 agggacgagg tgagacgtgt catcgacatt ttcggcactg gcattgtgat ttcgccgagc
     2461 cacgaagcca tactccccga cgttcccccg gagaacgtgg acgcgatgtt tgacgaggcc
     2521 cggacgtacc acgcgaaacc tcgatagaaa cagaaaggcc ctta
//
LOCUS       DAOTNG010000391         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_31531_length_2563_cov_4.440191, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000391 DAOTNG010000000
VERSION     DAOTNG010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31531_length_2563_cov_4.440191"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="P5179_13595"
     CDS             complement(<1..1593)
                     /locus_tag="P5179_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRS96297.1"
                     /translation="MLRRTTERYPAHVAMRMFRNGRYEELTYTDFSDRVDLLAAGLMR
                     LGIKPGAHVSMLGENRPEWGMAYLAVHRAGAVCVPLDSLQKVPEFRHNITDAEVEAVI
                     VSERFLDDILEVREKSGMPRLIICMDKTDREDVLVMSDVMKGELPSTWPEVKLTDLAA
                     IIYTSGTTGQSKGVMLTQRNIVEDAACTYQVLEFGSNDTFLSVLPLHHTFECTGGFLL
                     PLYAGASITYARSLKSRDIIDDIKNTGVTFMLGVPLLFEKMMEGIQRKLAQAPASKRI
                     LLAGLFSVEKLGRKLGLRLGYKLFKSLREKAGLATVRIFCSGGAALPPHVSIWYQSLG
                     FPLYQGYGLTETSPVSHVNLDPDYPASVGFPISCVETRIDRPGPDGHGEICLKGPTIM
                     AGYFKNEEATRAAIDPDGWFHTGDIGIEDADGRLYISGRQKNVIVTASGKNVYPEEVE
                     FHINQSPWVLESLVIGKPIANTTSEEVYAMVVPNKEYFDEQAQLRGQAFTEEEIEAKV
                     REEVEAAIANIADYKRPKRFEIR"
     gene            complement(1685..1912)
                     /locus_tag="P5179_13600"
     CDS             complement(1685..1912)
                     /locus_tag="P5179_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96298.1"
                     /translation="MALQKAREFTYRPPARRRAQIAGAEREKIRIPRAGNGKTGNASL
                     IRVVLMLGAVIALAIILSLGAGSPQGALRSR"
     gene            complement(1928..>2563)
                     /locus_tag="P5179_13605"
     CDS             complement(1928..>2563)
                     /locus_tag="P5179_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96299.1"
                     /translation="LHKPRQGNPDAEFEKRLVEIRTEIRRAESQAEAKELGVRESAFL
                     ELKGIYYDHTISAEDRNCAREHVLRLRDRHPTYSLLFLVAHRDDMHPVHKLSTQLIVD
                     IAEQDLRWASTAIWQYESPWITFSPHQVEYVVPFDKNAMETKVRAMSMHRSQEYRTRY
                     SDVARYSAQMRAETFPELLGGFGQGQSGYQWDYIEAFQELRPVRVLHMSGD"
BASE COUNT          503 a          803 c          675 g          582 t
ORIGIN      
        1 acggatttcg aatcttttcg gtcgcttgta gtcggcgata ttggcaatgg cggcctctac
       61 ttcctcccgg actttggctt cgatctcttc ttcagtgaaa gcctgcccgc ggagctgcgc
      121 ctgctcgtcg aagtactcct tgttcggtac caccatcgca tacacctctt cgctggtggt
      181 attggcgatc ggtttcccta tcacaagcga ttcgagcacc cacggactct ggttgatgtg
      241 gaactcaacc tcttcgggat acacattctt cccactcgca gtgacaatca cgttcttctg
      301 cctgccgctg atataaagcc gtccatcagc atcctcaata ccgatatcgc ccgtgtggaa
      361 ccatccgtcc gggtctatcg ccgcacgggt ggcctcttcg tttttgaaat aacccgccat
      421 gatggtcggc cctttgaggc atatttcccc gtgcccgtcc ggccccggcc ggtcaatcct
      481 cgtctccacg caggaaatcg gaaaaccaac gctcgccgga taatcgggat cgagattcac
      541 gtgagatacc ggcgacgtct ccgtaagccc gtacccctgg tacaacggaa aaccgaggct
      601 ctgataccag attgacacgt gtggaggcaa cgcagcgccg cccgagcaga atattctcac
      661 cgtggcgagc cctgcctttt cgcggaggct tttgaagagc ttgtacccca accggaggcc
      721 caacttgcgg ccaagttttt ccacactgaa cagacccgcg agcagaatcc ttttcgaagc
      781 cggcgcctgc gcaagcttcc gctgaatccc ttccatcatc ttctcgaaga gcagcggaac
      841 tcccagcatg aacgtgacgc ccgtgttttt tatgtcgtcg atgatatcgc gggatttcag
      901 gctcctcgca taggtgatgg aagcgcccgc atagagcggg agcaggaacc ctccggtgca
      961 ctcaaacgtg tggtgcagag gaagcactga aagaaacgta tcattcgagc cgaactcaag
     1021 aacctggtag gtacatgcgg catcctccac gatgttgcgc tgggtaagca tcacgccctt
     1081 tgactgcccc gttgtccccg aagtataaat gatggccgca agatcggtga gtttcacttc
     1141 gggccacgta ctcggcagtt cgcccttcat cacgtcgctc atcacgagga cgtcttcccg
     1201 gtcggttttg tccatgcaga tgatcaggcg gggcattcca gacttctcac gcacttccag
     1261 gatgtcgtcc aggaagcgtt cggaaacgat gactgcctcg acctccgcat cggtgatgtt
     1321 gtgccggaat tccggaacct tctgaaggct gtccagaggc acacagaccg caccggcgcg
     1381 gtgcaccgcg aggtacgcca ttccccattc cggacgattt tcacccagca tgctcacgtg
     1441 tgctccgggc ttgatcccca ggcgcatgag ccctgcggca agaaggtcca cacggtcgct
     1501 gaaatcggtg tacgtcagtt cctcgtacct tccgttacga aacatcctca tcgccacgtg
     1561 tgcgggatat ctttctgtag ttcggcgcag catctctact gtggtacaga cgcctaggtg
     1621 gtcgcgcaca gcgatttttc gtccggagcc agccatagat ttcacctcct gaggaaagca
     1681 accttcaccg acttcgcagg gcgccttggg gcgaccctgc gcctaacgaa aggatgatcg
     1741 ccaacgcgat aacggcgcca agcatcaaga ccacgcggat cagcgatgcg ttgcccgttt
     1801 tgccattccc tgcccgtggt atcctgatct tctctcgctc cgcacccgcg atctgggcgc
     1861 gtctgcgcgc cgggggacgg taggtgaact cccgcgcttt ctgcaaagcc atttcgcctg
     1921 cgccttctca gtcgcccgac atgtggagca cccttaccgg ccgaagctcc tgaaacgctt
     1981 caatgtagtc ccattggtac cctgattgac cctggccaaa tccgcccagc agctccggga
     2041 acgtttccgc cctcatctga gcgctgtatc gcgcaacgtc cgagtagcgc gttcgatact
     2101 cctgcgatcg atgcatcgac atcgcccgta ccttggtttc catcgcgttt ttgtcaaacg
     2161 gcactacgta ctcaacctga tgcggagaaa atgttatcca ggggctttcg tactgccata
     2221 tggcggtgga agcccaacgc aggtcctgtt ccgcgatatc aacgatgagc tgcgtggaga
     2281 gcttgtgcac cggatgcatg tcgtcccggt gcgcgaccag gaaaaggagc gagtaggtgg
     2341 gatgccggtc ccgaagacgc aacacgtgct cacgcgcgca attccgatct tccgcagaaa
     2401 tcgtgtgatc gtagtagatc cccttcagct caaggaatgc gctttccctg acgccgagtt
     2461 ccttggcttc ggcctggctc tccgcccgac gaatttccgt ccggatttcc accagcctct
     2521 tttcaaattc cgcgtcggga ttcccttggc gtggtttgtg cag
//
LOCUS       DAOTNG010000392         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_31775_length_2547_cov_4.493579, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000392 DAOTNG010000000
VERSION     DAOTNG010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31775_length_2547_cov_4.493579"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5179_13610"
     CDS             <1..274
                     /locus_tag="P5179_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS96300.1"
                     /translation="PSGLDSVRVFPQPFRPTHHHSLVFGGLPIGATLQIYRLDGSLIR
                     DFAATETGGITWDGKNGSGTAVSSGVYFYVVSSPSGRKTGKIAVVR"
     gene            552..1451
                     /locus_tag="P5179_13615"
     CDS             552..1451
                     /locus_tag="P5179_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96301.1"
                     /translation="MTDTTPSDRAVTRRIARDTLTPVEMNHGDVLEFQLVSGDAVRIA
                     LVSTSAHVVRTTLKHLKVEENAAQTLYRFDCVLRINGREHALEREIPSQRSFYEPWEI
                     DGVRIWFDAVDAIFEFLTEEHGECRPKKHARFAFQDATLRICPDSVHPWCPLPEGGLK
                     IEQCYRGEDCWMGPYNGASAHGGLDINHPRGTPLWAPIDIHDHSYFNSLAMGHNNNRW
                     RGIHRWPDGSEWILQAHHMTELTVPEHRPLKKGEQFAWGAGVLSGAVDHSHFVFKIRE
                     DDDIIALDPWILFWQMYRDQIAP"
     gene            1465..2436
                     /locus_tag="P5179_13620"
     CDS             1465..2436
                     /locus_tag="P5179_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96302.1"
                     /translation="MRVHIESVRCVFNNGEHNAFTDLCQFRGQLYLTFRSCPDGHMVF
                     PTSRIRVMRSSDGVTWEQVCEFGVPERDVRDPHFLVFGTRLFVYTGTWLCEENRDINH
                     HLGYCMWSDDGEVWQGPRFLEGTYGHYIWRAAAFGGKAYLCGRRKRAFARTADHGDGT
                     RITESALMESDDGFVFKPVGFFQHEYGDETAFLFEPDSSLLAIARDGAAKNALVCRSR
                     PPFAEWSRAKLERQIGGPMLAKWAGRYLVGGRKSEPGKPARTALWWLVNDQLEEIAEL
                     PSGGDTSYPGFVALSDSEGLLSFYSSHEGSGTGDPPSAIYLANLKAM"
BASE COUNT          504 a          746 c          806 g          491 t
ORIGIN      
        1 tccgagcggg ctggacagcg tccgagtctt cccgcagccg ttcaggccga cgcatcacca
       61 ttcactcgtc ttcggcggcc tccccatcgg ggcaaccctg cagatctacc gtctggacgg
      121 cagcctgatc cgagatttcg ctgcgacgga gaccggtggc atcacgtggg acgggaagaa
      181 cgggagcggc actgcggtct cgtcaggtgt ctatttctac gtcgtatcct cgccgtctgg
      241 taggaagacc ggaaaaatcg ccgtggtgag gtgagtcact ttgctggtgg ttggcaggaa
      301 ctcgaggggt ggattgttat ggattcccgc ctgcgcggga atgacggagc tatacggagc
      361 gtgggttcga cggggttacg ccggtctgct gagaacagcc acagagcgtt gttgcatggc
      421 gttctgtccg aggacgaaga gctatttccc tgcaggcggg agcagcaccg aataggctcc
      481 tggccttttg acgccgggca gcgcggggtt accctgatac cgtgcgtata catggatttg
      541 cgggaggtgc gatgaccgac acgactccga gtgaccgggc agtaaccaga cgaatcgcgc
      601 gggacactct cacacccgta gagatgaacc acggcgatgt gctcgaattc cagcttgtct
      661 caggagacgc ggtgcgcatt gcacttgtct ccaccagtgc gcacgtagtc cggacaacgt
      721 tgaagcacct gaaagtggag gaaaacgccg cacagacgct gtaccgcttt gattgtgtcc
      781 tgcggatcaa tggacgggag cacgcgctcg aacgtgagat tcccagtcaa cgcagtttct
      841 atgaaccgtg ggagatcgac ggcgtgcgaa tctggttcga cgcggtggac gccattttcg
      901 agttcctcac ggaggagcac ggggagtgcc gcccgaagaa acacgcgcgt ttcgcgtttc
      961 aggatgctac cctccgcatc tgcccggatt cggttcaccc ctggtgcccg ctgcccgaag
     1021 gcggcctcaa gatcgagcag tgctaccgtg gggaggactg ctggatgggc ccgtacaacg
     1081 gcgcctcggc gcacggcggc ctcgacatca accacccgcg cggaactccg ctctgggcgc
     1141 cgatcgatat ccacgaccac tcctatttca acagcctcgc catggggcat aacaacaacc
     1201 ggtggcgcgg catccaccga tggcccgacg gttccgaatg gatactgcag gcgcatcaca
     1261 tgaccgagct gactgtcccc gaacaccggc cgttgaagaa aggcgaacag ttcgcgtggg
     1321 gcgccggggt gttgtccggt gccgtggatc acagccactt cgtgttcaaa atccgcgagg
     1381 acgacgatat cattgccctt gatccgtgga ttcttttctg gcagatgtac cgcgatcaga
     1441 tcgcaccgtg aaggagggaa accagtgcgc gttcatatcg aatccgtccg ttgcgtgttc
     1501 aacaacggag aacacaacgc atttaccgac ctttgccagt tccgaggcca gctttacctc
     1561 acattccgtt cctgcccgga tggacacatg gtatttccca cctcgcgcat ccgggtgatg
     1621 cgaagctccg acggagtgac gtgggagcag gtgtgcgagt tcggagttcc cgagcgggac
     1681 gtccgcgatc cgcatttcct cgtcttcggc acacgtctgt ttgtgtacac ggggacatgg
     1741 ctctgcgagg agaaccgcga cattaaccac cacctcgggt actgtatgtg gtccgacgac
     1801 ggcgaggtgt ggcaggggcc gcgattcctt gagggcacct acgggcacta catctggcgg
     1861 gctgccgcat ttggtggcaa ggcatacctg tgtgggcgaa gaaagcgcgc attcgcccgc
     1921 accgcggacc acggcgacgg aacgcggatc acggaatccg ccctgatgga aagcgacgac
     1981 gggttcgtct ttaagccggt ggggtttttc cagcacgaat acggcgacga gacggcgttc
     2041 ctgttcgagc ccgacagctc tctcctcgcg atcgcacgtg acggcgcggc gaaaaacgcg
     2101 ctcgtgtgcc gttctcggcc accctttgcc gagtggtcac gggcgaagct cgagcgccag
     2161 atcggagggc cgatgcttgc gaaatgggcg gggagatacc tggttggcgg gcgaaaaagc
     2221 gaaccgggca agcccgcccg gactgccctc tggtggttgg tcaacgacca gttggaggaa
     2281 atcgccgaat tgccgagtgg aggagatacc tcctaccccg gtttcgtggc gctgagcgat
     2341 tccgaaggct tgctgtcgtt ctattccagc catgagggaa gcggcaccgg tgacccaccg
     2401 agcgcaatct atcttgccaa tctgaaggca atgtgacgcc gcgtgatcgc ggcataattc
     2461 aggcatcgaa aggagatctc acccatgcgc atcactgacg tcaagccgct cgtgctgggc
     2521 acgagctggc ggaacatcat tttcatc
//
LOCUS       DAOTNG010000393         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_32179_length_2523_cov_3.447731, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000393 DAOTNG010000000
VERSION     DAOTNG010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32179_length_2523_cov_3.447731"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..761)
                     /locus_tag="P5179_13625"
     CDS             complement(75..761)
                     /locus_tag="P5179_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS96303.1"
                     /translation="MLWYDGGHPGDIWRGAEMDAMVRALQPEIVTNGGPGTAGDFVTH
                     EQNTDGDPRPWEACVTMNDCWGYVPSEKGYKTVMQLIGTLVTCASKGGNLLLNVGPDP
                     EGRIPAAAVDRLRSIGKWMRVNGEAIYGSTRTVLRPPSEVGNATQVGNRQFLHIHAWQ
                     GSTLAMAWVGNRVTRARVLATGTEAQIEQKGDRVWLHGLPQYAPDPDISVIELEVEGE
                     PRYPELRFHF"
     gene            complement(679..1272)
                     /locus_tag="P5179_13630"
     CDS             complement(679..1272)
                     /locus_tag="P5179_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS96304.1"
                     /translation="MSTDRHEDPKAWFMQARFGMFIHWGVYAIPGRGEWVMYREHIPA
                     EEYAKLADQFNPRHYKPRDWVALAQDAGMRYMVLTARHHDGFCLWDTKTTDFSAPRTA
                     ARRDLLAEYVDACHSMGMRMGFYYSLADWRFPGGLYCYPRDPGDRLRTYGGSSARADS
                     RAADQLRQGRHAVVRWRSPRRHLARRGNGRHGQGTPA"
     gene            complement(1302..>2523)
                     /locus_tag="P5179_13635"
     CDS             complement(1302..>2523)
                     /locus_tag="P5179_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96305.1"
                     /translation="LVGCERGADGTEAKPHRALIIMFWSEPFALEIAKVKDSPNLWGY
                     WALDDTPGYALSAMRGLTSVVHEVDGRHPVCGGYSGPDTMFNFGPGACDIIVPYWYPV
                     LKWGYDRQMTSMDTQAILVYARRQVPGIPFMGLYQAFWGADWNKRGPLTAFEIREQAE
                     DFVREGAAGLMGFAVLHQTNPNDIFGGFNLDPEMTRAVREINDEVRRTCSLTVPPERP
                     EMAAIRIRPIGFQAREPEPQGIPPAWRIAFPFDAGESGDLDTNLAPDAGIDLTATYAG
                     KDGEQASWETIGTRGGVIAFLDLIGPVKKAENTIGYAVCTVTSAVERTVQVRLGAAKD
                     TLVRVNGKEIWRYTGSRGIHLDDNVAWTTLPAGRSEIVVKCYNRGGGWGFALRLADPD
                     GRPAAGLSFEPAAG"
BASE COUNT          425 a          876 c          730 g          492 t
ORIGIN      
        1 agaattgccg cgacgaggat cgccgtcgcg acgacaaacc atgtccagaa ctggttgctc
       61 accgcagcgc cgcctcaaaa atggaaccgg agttccgggt accgcggctc gccctcaacc
      121 tccagctcaa tgaccgagat atccgggtca ggtgcgtact gcggcagccc gtgcaaccac
      181 acgcggtcac ccttttgctc gatttgggct tcggttcccg ttgccagcac acgtgcccgg
      241 gtcacccggt ttcccaccca cgccatcgcc agcgtcgagc cctgccacgc gtggatgtga
      301 aggaactgcc ggttaccgac ctgcgttgcg tttcccacct cgctcggcgg gcgcagcacc
      361 gtccgcgtgg agccgtagat cgcctcaccg ttcacgcgca tccatttccc gatcgatcgc
      421 aaccggtcca cagccgccgc gggaatccgc ccttcagggt cgggacccac gttcagcagc
      481 aggtttccgc ccttcgacgc gcaggtgacc agggtgccga tgagttgcat caccgtcttg
      541 tagccttttt cgctcggaac gtatccccag caatcgttca tcgtcacaca ggcttcccat
      601 ggccgcgggt cgccgtcggt gttctgctcg tgcgtcacaa aatcccccgc cgtgccgggg
      661 ccaccgttgg tgacgatctc aggctggagt gccctgacca tggcgtccat ttccgcgccg
      721 cgccagatgt cgccggggtg accgccatcg taccacagca tgtctacctt gccgtagttg
      781 gtcagcagct cgcgaatctg cgcgtgcgct tgagccacca taggttcgta atcgatcgcc
      841 ggggtcgcgt gggtaacagt agagcccgcc ggggaaacgc cagtccgcca gcgagtagta
      901 gaaccccatc cgcatgccca tggagtgaca cgcatccacg tattctgcca gcagatcgcg
      961 ccttgcggcg gtcctcgggg cgctgaagtc cgttgtcttg gtgtcccaca ggcagaagcc
     1021 gtcgtggtgc ctcgccgtca gcaccatgta ccgcatgccg gcgtcctgcg caagagccac
     1081 ccagtcccgg ggcttgtagt gccgggggtt gaactgatcg gcgagcttcg catactcctc
     1141 cgcgggaatg tgctcccggt acatcaccca ttccccgcgc ccgggaatgg catatacccc
     1201 ccagtggatg aacatcccga accgtgcctg catgaaccac gccttcgggt cctcgtgccg
     1261 gtctgtgctc acgtgttttt ccttccccgc aaggtgacgt gtcatcccgc agctggctcg
     1321 aaactcaagc ccgctgccgg cctgccgtcg ggatcggcga gacgcagtgc aaatccccac
     1381 ccgccgcccc tgttgtagca tttgacaacg atctcagatc ttcctgccgg cagcgtcgtc
     1441 cacgccacgt tgtcgtcgag gtggatgcct cgggaccccg tgtaacgcca tatttccttt
     1501 ccgttgaccc gtaccagcgt atccttcgcg gcgccgagcc gcacctgcac cgtccgctcg
     1561 accgcgctgg tgaccgtgca caccgcgtac ccgatcgtgt tttctgcctt tttcactggc
     1621 ccgatgagat cgaggaacgc gatgaccccg ccacgcgtcc cgatcgtttc ccagctcgcc
     1681 tgctcgccat ccttgccggc atacgtcgcc gtgaggtcaa tgccggcatc gggagcgagg
     1741 ttcgtatcga gatcgccact ctcgcccgcg tcgaacggga acgcgatgcg ccacgccggg
     1801 gggattccct gtggctcggg ttcccgcgcc tgaaaaccga tggggcggat gcgaatcgct
     1861 gccatctcag ggcgttccgg aggcacggtg agggagcagg tgcgccgcac ttcatcgttg
     1921 atctctcgca ccgcgcgtgt catctccgga tcaaggttga acccgccgaa gatgtcgttg
     1981 ggattcgtct gatgtagaac cgcgaagccc atgagcccgg ctgcgccttc gcggacgaaa
     2041 tcctccgcct gttctctgat ttcgaatgcc gtcaacgggc cgcgcttgtt ccagtcagcc
     2101 ccccagaacg cctgatacag ccccatgaac gggattcccg gaacctgtct ccgggcgtac
     2161 acaaggatcg cctgcgtgtc catggacgtc atttgtcggt cgtagcccca tttcagcacg
     2221 ggataccagt acggcacgat gatatcgcac gccccggggc cgaagttgaa catcgtgtcg
     2281 gggccgctgt acccgccgca gacaggatgc ctgccatcaa cctcgtgaac gacgctcgtc
     2341 agcccgcgca tggccgagag cgcatacccc ggcgtgtcgt ccagtgccca gtagccccag
     2401 aggttcggcg agtccttcac tttcgcgatt tcgagggcaa aaggctccga ccagaacatg
     2461 atgatgagcg cccgatgcgg tttcgcttcc gtgccatccg caccgcgttc gcaacctacg
     2521 agg
//
LOCUS       DAOTNG010000394         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_32207_length_2521_cov_5.456204, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000394 DAOTNG010000000
VERSION     DAOTNG010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32207_length_2521_cov_5.456204"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>2521
                     /locus_tag="P5179_13640"
     CDS             102..>2521
                     /locus_tag="P5179_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRS96306.1"
                     /translation="MVYSLDGEWLIACDPRNEGRGQDWWAAVRPEACLTNVPWIIQGV
                     FPGYHGVAWYWRQFSVHRNPYPSGRYLLRFWAVDYLAEVWVNGQRVGAHEGAETPFVL
                     DITEAVVPDGKNLVAVRVLNPTNEPIDGITLNETPHRNKTVPIFAGASFNHGGIMDSV
                     ELIVAPAVRVADLVAHPDPVTGIVNVRVLLVNALAKAASCALRVSIAPSPSGETLETF
                     RGERMVDAGETWVSVQLKIDRPHLWQLNDPFLYHVTAEVSCATCSAASEYSVRCGFRD
                     FRFENGYFRLNGTRVFLRGSHTGNHAPIGQQLPPDPDMFRRDLLYVKSMGFNMIRFIA
                     GVATRYQLNLCDEIGLLVYEESYAGWLLGDSPRMKRLFDQSVCEMIRRDINHPSIVIW
                     GLLNETNDGPVFQHARNSLPAIRAIDPDRVILLNSGRWDGQQSIGSLANPGSTEWECC
                     LGGEAPGAAVTASTWGGYFERAGDAHAYPRVPHTAETISFLRRLGQDTGPVILTEYGI
                     GSAVDLWRVVRLFEQWRAEKCEDAVFYRDLLEQFLTDWRRWRLEEMFPRPSDYFAACV
                     RKMAGQRLLGLNAIRSNPHIIGHSMTGTVDQGLTGEGLFTTFRELKPGTVDAVADGLA
                     RLRWCLFAEPVHAYEGAPVRLEAVLADEDVLHAGAYPACFQVVDPSGARVFESHSSYR
                     VHDKGSERPLAAPVFADEVRISGGPGAYRFLAEFESGAAAAGGEAVFYVSRKPDTPLR
                     GVTVAVWGDDSDLENWLAAHGAEVVVPDRDTADQRCPIVLSKTPVHQGAPGGFDDLTR
                     RLQRGSTV"
BASE COUNT          466 a          736 c          779 g          540 t
ORIGIN      
        1 agtgcccggg tatgggcttc acgtttcggc ctgctgtttt gctcggttct taacagccgg
       61 atgaatctgg cacctgatcg cctttcggga aaggacttgc cgtggtatac agccttgacg
      121 gtgaatggtt gatcgcgtgt gaccctcgca acgaagggcg aggtcaggac tggtgggccg
      181 cggtccggcc cgaggcttgc ctcaccaacg tcccctggat catccagggc gtcttccccg
      241 gataccacgg ggtcgcatgg tactggcggc agttctcggt ccaccgaaat ccctacccct
      301 ccggaagata tctgctccgg ttctgggcgg tcgactatct tgccgaagtc tgggtgaacg
      361 gtcaaagggt tggtgctcac gaaggagcag aaaccccgtt cgtactggat atcactgaag
      421 cggtcgttcc ggatgggaag aaccttgttg ccgtgcgtgt gctgaacccg acgaacgagc
      481 caattgacgg gattaccctg aatgaaactc cccatcggaa caaaaccgtt cccatctttg
      541 ccggcgcatc attcaaccac ggcggcataa tggactcggt ggagctcatc gtcgcccctg
      601 cagtccgcgt cgcggacctt gttgcccatc cagaccccgt taccggtatc gtcaatgtgc
      661 gggttcttct ggtgaatgcc ttggcaaagg ccgcttcttg cgcccttcgc gtgagtatcg
      721 ctccatcccc gtccggagag actctggaga cgttccgcgg cgaaaggatg gtagatgccg
      781 gcgagacatg ggtgagtgtc caactgaaga ttgaccgtcc gcatctctgg caactgaacg
      841 accccttcct ctatcatgtt acggcggagg tctcctgcgc gacgtgttcc gccgcaagcg
      901 agtattccgt tcgctgcgga tttcgtgact tccgttttga gaatgggtat ttccgtttga
      961 acgggacacg ggttttcctc cgcggctcgc acacaggcaa ccatgcaccg atagggcagc
     1021 aactgcctcc ggaccccgac atgttccggc gggacctgct gtatgtcaag tccatgggat
     1081 tcaacatgat ccggttcatc gcaggagtgg ctacccgtta ccagctgaac ctgtgcgacg
     1141 agatagggtt gctcgtgtac gaagagtcat acgcgggatg gttgctcggg gattccccgc
     1201 gcatgaagcg tttgtttgat caatccgtat gcgagatgat ccggcgcgac atcaaccatc
     1261 ccagcatcgt tatctgggga ctcctgaatg agaccaatga cggcccggtg tttcagcacg
     1321 ccagaaattc cttacctgcc atccgggcga ttgacccgga tcgtgtcatc cttctgaaca
     1381 gcggcaggtg ggatggacag cagtcaatcg ggtcactcgc gaaccccggg tccacggagt
     1441 gggaatgctg tctcggcggg gaggcgcctg gcgccgcagt cacggcttcc acctggggtg
     1501 gctatttcga gcgtgcgggc gacgctcatg cgtaccctcg ggtgcctcac actgcggaaa
     1561 ccatttcctt cttacggcga ctcgggcagg atacagggcc cgttatcctt acggaatacg
     1621 gcatcggcag cgcggtcgac ctctggcgcg tcgtccggct tttcgagcag tggcgcgccg
     1681 aaaaatgcga agatgctgtg ttctaccgcg atcttctcga gcagttcctc accgattgga
     1741 ggcgctggcg gctggaggag atgttccctc gcccatcgga ttacttcgcc gcgtgtgtgc
     1801 gcaaaatggc cgggcaacga ttgctggggc tcaacgcaat ccgatccaac cctcacatca
     1861 tcgggcacag catgacaggt accgtggatc aggggctcac cggcgaaggg ctgttcacca
     1921 cgttcagaga actgaaaccg ggaacagttg acgccgtagc cgatggcctt gcgcgactgc
     1981 gttggtgcct cttcgcagaa cccgttcacg catacgaggg cgcgcctgtt cgtctggagg
     2041 cagtgcttgc ggacgaggat gtgcttcatg ccggcgcata tccggcgtgc ttccaggtgg
     2101 tggatccgtc gggtgctagg gttttcgagt cacattcctc gtaccgcgtc cacgacaaag
     2161 gctcagaacg cccgcttgcc gcgccggtgt tcgcggacga agtaagaatt agcggagggc
     2221 cgggtgcgta tcgctttctt gcggagttcg aatcaggtgc ggccgccgca ggtggagagg
     2281 cagttttcta cgtgtcgcga aaacccgaca cgccgttgcg cggcgtgacg gttgcggtgt
     2341 ggggtgacga ctcagacctg gaaaactggc ttgcggcaca cggggcggag gtcgttgttc
     2401 cggatcgcga taccgcggat caacgatgcc cgattgtcct ctccaaaacc ccggtccatc
     2461 agggcgcacc gggaggattc gacgatctca cccgtcgtct gcagcgaggc tcgacagtca
     2521 t
//
LOCUS       DAOTNG010000395         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_32456_length_2508_cov_3.317163, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000395 DAOTNG010000000
VERSION     DAOTNG010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32456_length_2508_cov_3.317163"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="P5179_13645"
     CDS             complement(<1..1836)
                     /locus_tag="P5179_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HRS96307.1"
                     /translation="MAKESTLSRKTQKRRTVGTYKYRDETGQVLFEVVRYEPKNFSMR
                     RPGEHGDWIPNIDSVRRVLYRLPEVIAAVQNNETVYVVEGEKDADRLASLGLCATTNP
                     QGAGQWKAEYSESLEAAHVVILPDNDEAGRRHAEKVAQSLQGKAVSIKVLPLPGLPDK
                     GDVSDWLDLDGANDARKIEELAEAAGKWQPGRGVSDQRDKSEFALTDYGNAERLASLF
                     GHHIRYCWAEKCWYVWSGKRWERDDGGGIQRMAQMTVRKIGKEALRCTDETKRQTIFR
                     WAAASEAVYHMRGMLDLARSLPGVPVLRQEFDTNSHTLNTGNGIVDLYTGELKAHNPS
                     EMVTRITNSDFDPTARHPAWDRLLKTVANRDVELEGFLQVAIGCSLVAGNVEEKLFLI
                     LGPPATCKTTFLEAIKSALGTYAASASLTAFARRNDPGAARGELVNAQGARLLLSSEA
                     DGDIPLVLSLMKAVTGRDTLTLEKKYQDAVELKPSYTWWIASNESPAFPAGDDGIWRR
                     IVCIPFTHVVPEQDRRPELKESLQRNAVARRAILRWCIEGARLYHQNGLVLPESVRTA
                     TAELRSERDPLSGFISDCCQLGKGNVTSSTDLLEASKAWAAANGKP"
     gene            complement(1839..2126)
                     /locus_tag="P5179_13650"
     CDS             complement(1839..2126)
                     /locus_tag="P5179_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRS96308.1"
                     /translation="MNASRLKSAIPPAEFYRVELPGMPLTKRANGWVDGGLCPFHDDH
                     NAGNFRINLDNGAFKCFSCGARGGDIIAFVMRRRKVSFPHAIAALRNQWGV"
     gene            complement(2123..2371)
                     /locus_tag="P5179_13655"
     CDS             complement(2123..2371)
                     /locus_tag="P5179_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96309.1"
                     /translation="MNQGEQTTPKQVQVPTIPRLIPLTKWNEYHPWPPPGGLRWLVFH
                     EKKNGFQAVIKRVGGRVLIDEQAFFDWVDEQNQTGGVR"
BASE COUNT          466 a          767 c          704 g          571 t
ORIGIN      
        1 cggcttccca ttggcggcag cccatgcctt cgacgcctcg agtaggtctg tggacgacgt
       61 gacgtttccc ttcccgagct gacaacagtc gctgatgaaa cccgacaacg ggtctctttc
      121 cgagcgcaat tccgcagttg ctgttctcac tgattcgggg agaaccagac cgttctgatg
      181 atacagccgg gcaccttcaa tgcaccaccg gaggatcgcg cgacgcgcca cagcatttcg
      241 ttgcagcgac tccttgagtt ccggtctgcg atcctgctcc ggaacgacgt gagtgaacgg
      301 tatgcacacg attcgccgcc atatcccatc atcgcctgct gggaaagctg gagattcatt
      361 ggatgcgatc caccaggtgt atgaaggctt cagttccacc gcgtcctggt attttttttc
      421 caacgtgagc gtgtcgcgtc ctgtgactgc cttcatcaag ctcagaacca acgggatgtc
      481 tccgtcggct tcgctcgaca agaggaggcg agcgccttgc gcgttgacaa gttcaccgcg
      541 cgccgcgccc gggtcgtttc gtctggcaaa cgcggtcaga ctcgctgagg ccgcgtaggt
      601 tccaagagcc gatttgatgg cctccaagaa ggtggtcttg catgttgccg gaggaccgag
      661 aatcaagaag agtttctctt ccacgttacc ggccacgagt gaacaaccga tggctacctg
      721 gaggaatccc tccaactcca cgtcccggtt cgcgacggtt ttgagcaaac ggtcccatgc
      781 gggatgccgc gcggtcggat cgaaatcgct attggtgatt cgggtgacca tttcagaagg
      841 gttgtgcgcc ttcagttcgc cggtatacaa atcgacgatc ccgttccccg tgttgagggt
      901 gtggctgttc gtatcgaatt cctgtctgag aacgggcacc cctggaaggg atctcgcaag
      961 gtcgagcatc ccgcgcatgt ggtacaccgc ttcggacgct gccgcccatc ggaaaatggt
     1021 ctggcgcttc gtctcgtcgg tgcatcggag ggcctccttg ccgatcttgc gaaccgtcat
     1081 ttgcgccatc cgctggatcc ctccaccatc gtcccgctcc catcgctttc cggaccatac
     1141 ataccagcac ttttctgccc agcaataacg gatgtggtgc ccaaagagcg acgcgagtct
     1201 ctccgcgttc ccgtagtcag tcaacgcgaa ctcgctcttg tctctctggt cgctgacgcc
     1261 gcgaccgggt tgccacttgc cagccgcttc ggcaagctcc tcgatcttcc gggcatcgtt
     1321 cgcgccgtct aagtccaacc agtcggacac gtcgcccttg tccggcaacc caggcagcgg
     1381 aagcacctta attgaaaccg ccttgccctg gagggactgc gcgaccttct cggcgtgtcg
     1441 gcgcccagct tcatcgttat cagggaggat cacaacatgc gctgcttcaa gcgactccga
     1501 atactcggct ttccattgtc cggcgccctg cgggttcgtc gtggcgcaaa gtccaagcga
     1561 tgcgagacgg tccgcgtcct tttcgccttc gaccacgtac actgtttcgt tgttctggac
     1621 cgcagcgatc acctccggca gtcggtacag gaccctccgc acgctgtcga tgttcgggat
     1681 ccagtcaccg tgttcgccgg gccttcgcat gctgaagttc ttgggctcat agcggactac
     1741 ctcgaacaac acttgccccg tttcgtcccg gtatttgtaa gttcccacgg ttctgcgttt
     1801 ttgcgttttt cgcgaaaggg tgctttcctt ggccatggct acactcccca ctgattgcgg
     1861 agtgccgcaa tggcgtgcgg gaaactcacc ttgcgacgac gcatgacgaa cgcaatgatg
     1921 tcaccgccac gcgcaccgca ggagaagcat ttgaaggctc cgttgtccag attgatgcgg
     1981 aagttgcctg cgttgtgatc gtcgtggaac gggcacagcc cgccgtccac ccaaccgttg
     2041 gcacgcttcg tcaggggcat tccgggcaac tcaacgcggt agaactccgc tggtgggatc
     2101 gctgatttga ggcgagaggc gttcatcgca cgcctcctgt ctggttctgt tcgtccaccc
     2161 agtcgaagaa ggcctgctca tcgatgagaa cacgccctcc cacacgtttg atcaccgctt
     2221 gaaagccgtt tttcttctcg tggaacacca accagcgcag acctccagga ggcggccaag
     2281 ggtggtactc gttccacttc gtgaggggga tgagtcgggg gatggtcggt acctgtacct
     2341 gctttggagt tgtttgctcg ccttgattca ttttctgcct cctggtttcg ggaaaaccga
     2401 gccagaccgt tctggcaccg ttctgatacc gataataccg gaggagacag ggggtgtaac
     2461 agagaaatga tgatcagtga ctaggcagga agaacactac ccagttac
//
LOCUS       DAOTNG010000396         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_32683_length_2494_cov_5.471915, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000396 DAOTNG010000000
VERSION     DAOTNG010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32683_length_2494_cov_5.471915"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="P5179_13660"
     CDS             complement(<1..838)
                     /locus_tag="P5179_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRS96310.1"
                     /translation="MGSKTLTPASAVFLVLLFGVFAVFLVYPVGYVLESAFYREGVFT
                     LEFFRLMFTNPVHREAIINSTMLAITVTAVTTVIALPISFVMVRRDFPGKPLLNGLLL
                     VPMVMPPFVGAVGMQQLLAKFGSVNLLLIRIGLMSPRDPFDWLGTGFWAVVLLSVLHL
                     YPIMYLNVAAALANVDPSLEEAGRNMGASGFRLFRSVTFPLMLPGYFAGAIIVFIWAF
                     TDLGTPLIFNYSRVVPVRIFEAVSDIHENPMGFALVVLVLAMTVVFFAISKKVLGSSN
                     YQM"
     gene            complement(848..2263)
                     /locus_tag="P5179_13665"
     CDS             complement(848..2263)
                     /locus_tag="P5179_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRS96311.1"
                     /translation="MSDTGKVTYIMRFGGTRARRFWSTTLTWVLFCVPAAVVAGCSAA
                     GRADDELVILSPHWEGIQQEFERGFAEYWKAKTGRSVDITWLDQGGTSQILRFISSEY
                     KNSPEGIGIDLMFGGGVDPYLKLVKDGVTHPFTLPDSVLKRIPAHFAGMPMYDSTGRW
                     YGATMSGFGIVFNKAVARRLGRFAPHTWKEMGDPAWRGWIGAGDPRLSGSIHMAYELM
                     LQAYGWEEGWKTITAIAANTRSFARGAAEIPLSVARGDAVAGMCIDFYAWSEVTHVGS
                     SELSFVYPVNLTAVNPDAICILRGAPHLNLAKEFVTFVMSEPGQRLWSFKKGAPGGPL
                     KNQLNRFTVMPDLYERYPEHIAVDENPFSWRITFQYDSGLGSRRWSVVNDLIGAQLID
                     SHRELEQAWEALRQRGMPEEGLSLLASQPVSPEQALALAEQWKDPAVRNAYLSQWTAF
                     ARDKCRAVIRGEYGGNRQYTH"
     gene            complement(2265..>2494)
                     /locus_tag="P5179_13670"
     CDS             complement(2265..>2494)
                     /locus_tag="P5179_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRS96312.1"
                     /translation="GAEEALSGGILRGVVQSIIYLGDTEQYVVRLADGKLVRSVEANP
                     ARPRARTGEEVHLTFRSEDAIALPPDTSNGG"
BASE COUNT          552 a          750 c          706 g          486 t
ORIGIN      
        1 tcatctggta attgctcgat ccaagcacct tcttgcttat cgcgaagaat acgacggtca
       61 tcgcgagaac gagaacaacg agcgcgaagc ccatcgggtt ttcgtggatg tcgctcactg
      121 cctcgaagat tcttaccggt actacgcggg agtagttgaa aatgagggga gtaccgaggt
      181 ccgtgaacgc ccagatgaac acgatgatcg cgccggcgaa gtaacccggc agcataagag
      241 ggaaggtaac ggaacgaaac agcctgaaac ccgaagcccc catgtttctg cccgcttctt
      301 cgaggctcgg atcgacgttg gcgagagccg ccgccacgtt aaggtacatg atcggataga
      361 ggtgcagaac gctcagcaac actaccgccc agaaacccgt accgagccag tcgaacgggt
      421 ctcgcggaga cataaggccg attctgatca agagcaggtt gacggaaccg aatttggcaa
      481 gaagctgctg catgccgact gcgccgacga acggtggcat caccatggga acaagcaaga
      541 ggccgttcag cagcggcttc ccggggaaat cccttctcac catcacgaat gaaatcggca
      601 gcgcgatgac ggttgtcacg gcggtaacgg ttatggccag catcgtgctg ttgatgatgg
      661 cctcccggtg aaccgggttg gtgaacatca gcctgaagaa ctcaagcgtg aacacaccct
      721 cacggtagaa ggcactctcc agaacgtatc ccacggggta tacgagaaac acggcgaaca
      781 cgccgaaaag gagcaccagg aagactgcag atgcgggggt caaagttttg ctgcccatgt
      841 cgagatccta gtgtgtatac tgtctgttgc cgccgtattc cccgcggatc accgcccggc
      901 atttgtcccg cgcaaatgcc gtccattgcg agaggtacgc atttcgaacg gcggggtctt
      961 tccattgttc cgccagcgca agcgcctgtt ccggcgacac tggttgcgag gcaagaagag
     1021 acagcccttc ctcgggcata ccgcgctggc ggagcgcttc ccacgcctgt tccagctcac
     1081 ggtgagagtc gatcagctgc gcgccgataa ggtcattcac cactgaccat cgtcgcgaac
     1141 cgaggccaga gtcgtactga aaggttattc gccacgagaa agggttctcg tccacggcga
     1201 tgtgttcggg atagcgctca tacaggtccg gcattacggt gaagcgattg agctggtttt
     1261 tcaaaggacc gcccggcgcg ccttttttga acgaccacag gcgttggcca ggttcgctca
     1321 tcacgaatgt cacgaactcc ttcgccagat tcagatgtgg tgcaccgcgg agaatgcaga
     1381 tggcatcggg gttgaccgcc gtaaggttga ccggatagac gaaactcaac tcactcgaac
     1441 caacgtgtgt aacttcggac cacgcgtaga aatcgatgca catcccggcg acagcgtcgc
     1501 cgcgcgccac ggagagaggt atttcagcgg cgccgcgcgc gaacgatcgg gtgttggcgg
     1561 ctatcgcggt gatggtcttc cagccttcct cccacccgta agcctggagc atcagttcgt
     1621 acgccatgtg gatgcttccc gagagcctgg gatcacccgc tccaatccag ccgcgccatg
     1681 ccggatcgcc catttccttc cacgtgtgag gcgcgaacct tccgagtcgt ctggcaacag
     1741 ctttgttgaa cacaattccg aacccggaca tggtcgcgcc gtaccacctg ccagtggagt
     1801 cgtacatcgg catgccggca aaatgggctg gaattctttt cagcactgaa tcgggaagcg
     1861 tgaatggatg tgttacgccg tccttgacaa gtttgaggta cgggtcgacg ccgcccccaa
     1921 acatgagatc gataccgatt ccttccggcg agttcttgta ttctgaactg atgaaccgaa
     1981 gaatctgcga tgtccccccc tgatcaagcc atgtaatgtc cacggatcga ccggttttcg
     2041 ctttccagta ctctgcgaat cctcgttcaa attcctgctg gataccttcc caatgaggag
     2101 aaagaatcac aagttcgtcg tccgcccgtc ccgccgccga acacccggcc actactgcgg
     2161 ctggcacgca gaagagcacc cacgtcagcg tcgtgctcca gaacctgcgg gcacgcgttc
     2221 cgccgaaccg catgatgtac gtgaccttgc ctgtgtccga cacctcagcc tccattgctc
     2281 gtgtccggcg gcaacgcaat cgcgtcctcg gaacggaacg tcaggtgaac ctcctcaccg
     2341 gtgcgggcgc gcggccgcgc gggattagcc tccacgctgc gaacgagttt gccatcggcc
     2401 aagcgcacga cgtactgctc tgtgtcgcca aggtatatga tggattggac aacgccgcgc
     2461 agaattcccc ctgacaaggc ttcctccgcc cctc
//
LOCUS       DAOTNG010000397         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_33301_length_2460_cov_3.427027, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000397 DAOTNG010000000
VERSION     DAOTNG010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33301_length_2460_cov_3.427027"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="P5179_13675"
     CDS             complement(<1..600)
                     /locus_tag="P5179_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS96313.1"
                     /translation="MTPQITVVSGKGGTGKTTLTALWGSMADRPVLVDADVDASNLPL
                     AYPHETREQHAFVGRDVAVVNEQLCKNCGVCARACRFDAFFQVEGQFRVDPLACEGCT
                     LCTLMCPLDAITMKPHVSGTWSVSETGFGPLVHAQLGIAEGNSGKLVTQVRRAGEKLA
                     EERAAAVILVDGPPGIGCQTTAAMAGASLVVLVTEPSASG"
     gene            complement(597..1451)
                     /locus_tag="P5179_13680"
     CDS             complement(597..1451)
                     /locus_tag="P5179_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS96314.1"
                     /translation="MIVSVASGKGGTGKTTIAVALAQVAPHSAYVDCDTEAPNGHLFL
                     RPNISHRKPVSRTVASINVNTCTWCGECMEKCAYHAIVIAHATRKAVVFEDLCMGCGV
                     CAFVCPARAITEEERHAGIVSNGHSGRIRTREGRMAVEAPHAHPVLTETLKELPDAPC
                     TILDAEPGVTGRVVTTVRASDVCVLVTEPTPFGLHDLELAVTMTQHVGVPVGVIINRS
                     TIGDNAVRDFCRVRNIPVLLEIPFDRRIAAGYAVGKTLVEILPDMKSRFAEVLRHVER
                     IVTERGRS"
     gene            complement(1448..2002)
                     /locus_tag="P5179_13685"
     CDS             complement(1448..2002)
                     /locus_tag="P5179_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRS96315.1"
                     /translation="MKIAVSSSGQELDSPVDPRFGRCTHFVIVDSDTDDYEAVENAAS
                     EAGSGAGIAAASALAKAGAEVVLTGNLGPNAARTLSTLGIKAYRAEGDTVGESVTAFI
                     DGQRFEITSPTVAAKSGLNASPTVFPSRPFGGSTGGGIGRGMGGGGSMGGGGGRGMGG
                     GGGRGAGGGRGAGGGRGAGGGRGR"
     gene            complement(2032..2388)
                     /locus_tag="P5179_13690"
     CDS             complement(2032..2388)
                     /locus_tag="P5179_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRS96316.1"
                     /translation="MKIAIPVETSDGLRSIVAGHFGHAPYYAFVDGKTKQCAVVPNGS
                     SHNGGDKLPPEWLFDEGVNVLICNGLGARAIGLCTQLGIEVYITEEQTVAESLGAFEA
                     GTLTRAGSEDACAHEH"
BASE COUNT          446 a          875 c          650 g          489 t
ORIGIN      
        1 ccctgacgcc gacggttcag ttaccagcac cacaagagat gctcccgcca tcgccgccgt
       61 cgtctggcat ccaatcccgg gagggccgtc aaccagaatc actgccgccg cccgctcctc
      121 tgccagcttc tcccccgccc ggcgcacctg cgtcaccagt ttgccggaat tcccctcagc
      181 aattcccagt tgagcatgga cgagaggtcc gaacccggtt tcgctcacag accacgtccc
      241 ggagacgtgc ggtttcatgg ttatcgcgtc gagcgggcac atcagcgtgc acagcgtgca
      301 tccttcgcac gcaagcgggt ccacccggaa ctggccttca acctgaaaaa atgcatcaaa
      361 cctgcacgcc ctcgcgcaca caccgcagtt cttgcacaac tgctcgttca ccacggccac
      421 atcgcgcccg acaaacgcgt gctgctcccg ggtctcgtgg gggtacgcca ggggaaggtt
      481 cgacgcgtcc acatcagcat ccacgagcac cggcctgtct gccatgctgc cccaaagagc
      541 agtaagcgtc gtctttccag ttccgccttt cccgcttacc accgtaatct gcggagtcat
      601 gatcgccccc tctcagtgac gatccgctcg acatgcctga gaacctcggc aaatcggctt
      661 ttcatgtccg gcagaatctc aacaagcgtt tttcccaccg cgtatcccgc tgcaatgcgt
      721 cgatcaaacg gaatctccag caaaacgggt atgttccgaa cccggcaaaa gtcgcgtacg
      781 gcgttgtcgc ctatcgttga acgattgatg atgacgccga ccggaacccc gacatgctgc
      841 gtcatcgtca cggccagctc cagatcgtgc agcccgaacg gtgtgggctc ggtgacaagg
      901 acgcacacgt cgcttgctct gaccgtcgtg acgacccgac ctgtaacgcc cggttccgcg
      961 tcgagaatgg tgcacggcgc gtcaggtagc tccttcaatg tctcggtcag caccggatgt
     1021 gcgtgggggg cttccactgc catgcggccc tcccgcgtac gaatccgccc gctgtggccg
     1081 ttcgagacga tgcccgcatg ccgttcttct tcggtgatcg cgcgcgccgg gcacacaaac
     1141 gcacacacgc cgcaccccat gcacaggtcc tcaaatacaa cagcttttct cgtcgcgtga
     1201 gcgattacga tggcgtggta agcgcacttc tccatgcatt caccgcacca ggtgcacgtg
     1261 ttcacgttga tcgaggctac cgtacggctc accggtttcc ggtgtgaaat gttcggccgc
     1321 agaaacagat gcccgttcgg cgcctcggtg tcgcagtcaa catacgcgct gtggggcgcc
     1381 acttgcgcca gcgcaaccgc aatcgtggtc ttgccggttc cgcctttccc gcttgctacg
     1441 cttactatca tcttccgcgt ccgccaccgg cccctctgcc gccgccagca ccacggcctc
     1501 cacctgcgcc tctgccgccg ccgccaccca taccgcggcc gccgccgcca cccatgctcc
     1561 cgccgccacc catgccgcgt ccgattcctc cgccagtgct gccgccgaac ggacgagagg
     1621 ggaataccgt gggagacgcg ttaaggcccg atttcgcggc gacagtcggc gaggtgattt
     1681 cgaaccgttg tccgtcaatg aaagcggtca ccgattcgcc caccgtgtcg ccttctgccc
     1741 ggtacgcctt gatgccgagc gtggagagcg tgcgcgcggc attgggcccg agattccccg
     1801 tcaaaacaac ctcggcgccc gccttcgcca gagcgcttgc cgccgcgatc cccgcgccac
     1861 tccccgcttc acttgccgca ttttcaacgg cttcgtagtc gtcagtgtcc gagtccacga
     1921 ttacgaaatg agtgcatcgt ccgaatcgcg gatctaccgg cgagtcaagc tcttgaccag
     1981 aactgcttac tgcgatcttc atcggtctca cctttcgtca tgtcgtggaa tctaatgctc
     2041 gtgggcgcac gcatcctcgg aaccagctct ggtcaaggtc cccgcttcaa acgcgccgag
     2101 ggactccgca accgtctgct cctcggtgat atagacctcg attcccagtt gtgtgcacag
     2161 gccgatggcg cgtgcgccca gcccgttgca gatgaggacg ttcacgcctt catcgaacaa
     2221 ccattccggg ggcagtttgt ccccgccatt gtgcgaactc ccgtttggaa cgacggcgca
     2281 ttgcttcgtc ttgccgtcga cgaacgcgta gtacggcgca tgcccgaaat ggccggcaac
     2341 gatactcctc aggccatcgc tcgtttctac cgggattgcg atcttcatga agtccttcct
     2401 ttcctgtgaa taggttccaa ccagaacact cctcggatat caaaccctcc agcgtccgcc
//
LOCUS       DAOTNG010000398         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_33764_length_2436_cov_4.212516, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000398 DAOTNG010000000
VERSION     DAOTNG010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33764_length_2436_cov_4.212516"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5179_13695"
     CDS             complement(<1..525)
                     /locus_tag="P5179_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96317.1"
                     /translation="MAIRKLEPVRVNPVLRHPDEQRVSLDGRWLFRLDPEDIGVRERW
                     FRDPAVLDASVQVPGCWQGQGHGGDGNDRVWDFGLEERTFRATYKGTGWYGRAFTPPD
                     EWQGRRVQVLFGGVHPSAEVWLNGERLGENGMPFVPFGFDITSNLRFCSENLLVVRVH
                     EAARIYGLAYSWQGN"
     gene            complement(558..1667)
                     /locus_tag="P5179_13700"
     CDS             complement(558..1667)
                     /locus_tag="P5179_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96318.1"
                     /translation="MFNAFPRDETITRRERFCRLYDFEPVDSHVRWEAVAFWGDTARD
                     WVAEGGLPEDADPMDYYGMDPRNGISGNLGFTSMGLSGPRPETHVIAEEGRTRVYRND
                     LGQVWRDRVDGVSMPQWIRFPVESRDDWERIIKPRLKPEDHDFGNLDEEAVRSRTSQD
                     AWGLWIVGLYAFWRNFWGEEKLAFAFYDQPDVLHDMAKCWLTMHCECTPRVLDTARID
                     YVMFHEDMAFRNGPLIGPQLFDSFMAPYYRELFVHLRNRGQHRFLLDSDGNNGPILER
                     FADLGMNGLYPFEAAAGCDAIAFRKEHPRFFVWGAIDKRVLLGNKDDVEREVMAKVPA
                     LWAGGGFIPSIDHSVPPCSQENFEHFINLVRSICR"
     gene            complement(1750..>2436)
                     /locus_tag="P5179_13705"
     CDS             complement(1750..>2436)
                     /locus_tag="P5179_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS96319.1"
                     /translation="RTIGDVIAELERQGIRQNTLVVFTSDNGMNMGHHGLYGKGNATF
                     PANMFDTSVKVPMIVSRPGSVPSGVVCDEMLSHYDLLPTFLDYVNAENPVAGETPGVS
                     FVPILRGDEFARRDAVVVFDEYGPTRMIRTKEWKYVHRYPYGPHELYDLVSDPEERDN
                     VVEDARCQNVLRDLRARLSEWFVQWADPARDGSREPVRGVGQHDLAGPAGAGVVAFNQ
                     DFRFLSTSHG"
BASE COUNT          497 a          794 c          676 g          469 t
ORIGIN      
        1 gttgccctgc caactgtacg ccaggccgta gatccgggcc gcctcgtgca ccctcactac
       61 cagcaggttc tcggaacaga aacggaggtt gctcgtgatg tcgaacccga agggaacgaa
      121 gggcatgccg ttctcgccga ggcgctcgcc gttcaaccag acctccgcac tcggatggac
      181 gccaccgaaa agaacctgca cgcgtcttcc ctgccactcg tccggtgggg tgaacgcgcg
      241 tccataccac cccgtgccct tgtaggtggc gcggaaggtc cgttcctcaa gtccgaaatc
      301 ccacaccctg tcgttgccat cgccgccgtg gccctggcct tgccagcacc cgggcacctg
      361 cacggaagcg tcgagaaccg cgggatctcg gaaccaacgt tcgcggacgc cgatatcctc
      421 gggatcgagg cggaacagcc accgaccgtc gaggctcacc ctttgctcat ccggatggcg
      481 gagcaccggg ttcacgcgta ctggttcgag cttccggatc gccattgcgg attcccttcc
      541 agatcggttg ctgcgattca tctgcagatg ctccggacga ggttgatgaa gtgctcgaag
      601 ttttcctgac tgcacggcgg aacagagtgg tctatggaag ggatgaagcc gccccccgcc
      661 cacagcgcgg gaactttggc catcacttcc cgctccacat cgtctttgtt tcccaaaagc
      721 acacgtttgt cgatcgcgcc ccagacaaaa aaccgggggt gttccttgcg gaacgcgatc
      781 gcgtcgcaac cggcggcggc ttcaaagggg tagagaccgt tcattccgag gtcggcaaac
      841 cgctccagaa tcggtccgtt gttgccgtcg gagtccagga gaaaccggtg ttgcccgcga
      901 ttcctaagat gaacgaacag ctcccggtaa tagggcgcca tgaatgaatc aaaaagctgc
      961 ggtccgatga gggggccgtt gcggaacgcc atgtcctcgt ggaacatgac atagtctatc
     1021 cgggcggtat cgagaacacg cggcgtgcat tcgcaatgca tcgtgagcca gcatttcgcc
     1081 atatcgtgca acacgtccgg ttggtcatag aacgcgaacg caagtttctc ctccccccag
     1141 aaattcctcc agaacgcgta caggccaacg atccacaacc cccacgcgtc ctgggaagtt
     1201 cgcgaccgga ccgcttcctc atccagattg ccaaagtcgt gatcttcggg tttcaggcga
     1261 ggcttgatga tgcgctccca atcgtccctc gattccacgg ggaagcgtat ccactgcggc
     1321 atgctgactc catccacccg gtcacgccat acctggccga ggtcgttccg gtacaccctg
     1381 gtacggcctt cttcggcgat cacatgagtt tccggcctcg gacccgagag ccccatggaa
     1441 gtgaacccga ggttgccact gatgccgttc ctcgggtcca tgccgtagta atccatgggg
     1501 tccgcgtctt ccggcagccc gccttccgca acccaatccc gcgcggtgtc accccaaaac
     1561 gccactgctt cccatcgcac gtgcgaatca acaggctcga agtcgtagag gcggcagaaa
     1621 cgctcgcgcc gcgtgattgt ttcgtctctc gggaacgcgt tgaacatgag ttagttcctt
     1681 ccctttcgtc ccagcagcaa ccaccccgca cgcggaatgc ctgacgtcgc gcgctccgaa
     1741 tgaacgctac tacccgtgag atgtgctgag aaagcggaaa tcctgattga aggcgaccac
     1801 tcctgcgccg gccgggcccg caagatcgtg ctggccaacg ccgcggaccg gttctcgcga
     1861 cccgtctcgc gccggatcgg cccattgaac gaaccactcg gacaggcggg cccgcagatc
     1921 acgcagaaca ttctgacaac gtgcatcttc cactacgttg tcgcgttcct ccggatcgga
     1981 aacgaggtca tacaattcgt gaggaccgta cgggtaacga tggacgtatt tccactcctt
     2041 cgtccttatc atgcgcgtcg gcccgtattc atcgaaaacc accaccgcgt cgcgacgagc
     2101 gaactcatcc ccacgaagga tcgggacaaa actcacaccg ggagtttcgc ccgcaacggg
     2161 gttttccgcg ttcacgtagt cgagaaacgt gggcagaagg tcgtagtggc tcagcatttc
     2221 gtcgcacaca acaccggaag gaacagaacc aggacgggag acaatcatcg ggaccttcac
     2281 cgacgtgtca aacatgttcg ccggaaaggt cgcgtttccc ttgccataga gaccgtggtg
     2341 ccccatgttc atcccgttgt cgctcgtgaa cacgacgagt gtgttctgcc tgatgccctg
     2401 cctctccagt tctgcgatca catcgccgat agtgcg
//
LOCUS       DAOTNG010000399         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_33883_length_2429_cov_4.787279, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000399 DAOTNG010000000
VERSION     DAOTNG010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33883_length_2429_cov_4.787279"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="P5179_13710"
     CDS             <1..486
                     /locus_tag="P5179_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96320.1"
                     /translation="GLSGHMGKQDWNDGTGTINSLAGAFDYDVRRGPFRIVGEFASGQ
                     SLGGINALTKFHQDPATGKRYAETGYMGWFEATYKFSPKWMASVAAGQEKADWNATHT
                     AMLTRQTMDRNRAGYVNVVWQPWAPFRVITQLDVIRSHYIAPAVEHKVEVLSVGAMLI
                     F"
     gene            623..1591
                     /locus_tag="P5179_13715"
     CDS             623..1591
                     /locus_tag="P5179_13715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS96321.1"
                     /translation="MQYRDLGKTGLRVSEIGFGAWAIGGGFEIGGKGIGYGATEDETS
                     IRAIHRALDLGVNLYDTADAYGAGHSESLLGKALQGRWSSCYVATKVGNERRDPLPGR
                     KNFTREYILSACERSLKRLRKDVIDLYQLHNPPPEVGDGDEVFETLSILREQGKIRFV
                     GVSVTTPAEGVSYIRRGVVDCLQIYYNAQTREAETELFPLCAREKIGTLIRAPLSSGI
                     LAAKFTKDTVFPPDDHRGNWLKGELLARAVAEAEALRKAAAPLPPAEAALRFILNQPA
                     VTCIIVGAKTPEQVERNCAASDGKGLATPVENAIRAVLPGDFPRQT"
     gene            1723..>2429
                     /locus_tag="P5179_13720"
     CDS             1723..>2429
                     /locus_tag="P5179_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96322.1"
                     /translation="MDYEPVKFGVVGLGMGQHHCKAIAAANGAELVAVCDIDMERTNK
                     MVQTYGCKGYSSYAEFLNHPGMEAVCICVPSGMHADFGIQAIQTGKHLVVEKPPDVKA
                     AKVQALIDAAAERGVKMEQIFQQRTSPLNRRIKDVIDSGRLGKLVGINALLPWYRAQS
                     YYEDVAHGRWKGTWALDGGGSLANQGVHTMDIVLYFFGRVKSVFGKFGVFTHDIEAED
                     HTVAILKFENGAMGTIMT"
BASE COUNT          539 a          645 c          762 g          483 t
ORIGIN      
        1 ggcctttcag gacacatggg gaaacaggat tggaacgacg gaacgggcac tatcaactcc
       61 ctggcaggcg cgttcgacta tgacgttcgg cgcggaccct tcagaatcgt cggggaattt
      121 gcctctgggc aaagcctcgg cggcatcaat gctctgacga agtttcatca ggaccccgcc
      181 actgggaagc gttatgccga gaccgggtac atgggctggt ttgaggcgac gtacaagttt
      241 tcgccaaaat ggatggccag tgttgcagcc ggccaggaaa aagccgactg gaacgccacg
      301 cataccgcga tgctgacccg gcagacaatg gaccggaacc gggcgggcta cgtgaatgtt
      361 gtgtggcagc cgtgggcgcc gttccgtgtc atcacccagc tggacgtgat tcggtcgcat
      421 tacattgccc cggcagtgga acacaaagtg gaagtgctca gcgtgggagc catgctgatt
      481 ttctagttgt tgcttctggt agccgggcgc gccgtctcgc accagttggt gcgggcggcg
      541 cgctggctgt tgatggaaga accctgtggc ggtccttcgg gcacggcatc gggttccctg
      601 ttcttacagg agggtgttcc aaatgcaata tcgggacctt ggcaaaacgg gactccgcgt
      661 ctctgaaatt ggattcggtg cctgggcgat tggcggaggt tttgagatcg gtggaaaagg
      721 tatcggctac ggagcgacgg aagatgagac gtcgatcaga gcaatccatc gggcgctcga
      781 tctgggtgtg aatctgtatg acactgctga cgcatacggg gcgggacaca gcgaatcact
      841 tcttggcaaa gctcttcagg ggagatggtc ttcgtgttac gttgccacaa aggttggcaa
      901 cgagcggcgc gatcctcttc ccgggcgcaa gaattttacc cgcgagtaca tcctgtcggc
      961 atgcgaacgc agcctcaagc ggctccgcaa ggacgtaatt gatctttacc agcttcacaa
     1021 cccgccgcct gaggttggag atggggacga agtattcgaa acactctcga ttcttcgtga
     1081 gcaggggaag atccgttttg tcggcgtttc cgtgaccacc cccgcagagg gcgtttcgta
     1141 catccggcga ggtgtcgtag actgccttca gatttattac aacgcgcaga cgcgtgaagc
     1201 ggagaccgag cttttccctc tttgcgcaag ggagaagatc ggtaccctta tccgcgctcc
     1261 tctaagctcg ggaatactgg cggcgaagtt cacgaaagac acggtgttcc cgcctgacga
     1321 ccatcgcggc aactggctca aaggcgagtt gctcgcccgc gcggttgccg aagcggaagc
     1381 actgcgaaag gctgcagcac ctttgccgcc cgcggaagcc gccttgcgct tcattctgaa
     1441 ccagccagca gtcacgtgca tcatagtggg cgccaagaca ccggagcagg tggaacgcaa
     1501 ctgcgcagcg tcagacggga agggccttgc aaccccggtg gagaacgcaa tccgcgctgt
     1561 cctgccaggc gattttccgc ggcagacctg aaaacccctt agggacagca ggccggggaa
     1621 cgtataatca atacgataac gcccttttgg aaacctttcg agaaacagaa agctggggcg
     1681 acctcgtttt gttttcccgc acagatgatg gagctgtgaa cgatggatta cgaacccgtg
     1741 aaattcggtg tcgtcggcct gggaatgggg cagcatcact gcaaggcaat cgcggcggcc
     1801 aatggcgcgg aactggttgc ggtatgcgac atcgacatgg agcgcacaaa caaaatggtt
     1861 cagacgtacg gctgcaaagg gtattcctcg tacgccgagt tcctgaacca cccgggtatg
     1921 gaagcagtgt gcatctgcgt tccgagtggt atgcacgccg atttcgggat acaggcgatt
     1981 cagaccggca agcatctcgt cgtggagaaa ccaccggacg tcaaggctgc aaaggttcaa
     2041 gccctgatcg acgcggcggc agaacgcggc gtgaaaatgg aacagatctt ccagcagcgc
     2101 acgagcccct tgaatagacg gatcaaggat gtcatcgaca gtggacgact cgggaaactg
     2161 gtcggaatca acgctttgct gccctggtac cgtgcccaga gctattatga ggacgttgcg
     2221 cacggtcgtt ggaagggaac ctgggcgctg gacggagggg gttcgcttgc caaccagggc
     2281 gttcacacga tggatatcgt gctgtatttc ttcggaaggg tgaagtctgt cttcgggaaa
     2341 ttcggcgtgt tcacacacga catcgaagcg gaagaccaca ctgtggcgat cctgaagttc
     2401 gaaaatggcg ccatgggaac catcatgac
//
LOCUS       DAOTNG010000400         2428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_33911_length_2428_cov_6.005478, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000400 DAOTNG010000000
VERSION     DAOTNG010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33911_length_2428_cov_6.005478"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="P5179_13725"
     CDS             <1..467
                     /locus_tag="P5179_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96323.1"
                     /translation="LHNTPYEAWTSYRVTGLDLRITARWGRYLVGDRAWRLDVERSFG
                     ETKLTVFGIKSDGYSDYLKRVDRQNTRLLGGVRMEIPLPPRERGMPERFRVTSTPAFA
                     WSYRYRVGDVGVDLVTKQSVEDHVGAYNPVAVLNNLERARSQVRARQSEACQ"
     gene            497..1798
                     /locus_tag="P5179_13730"
     CDS             497..1798
                     /locus_tag="P5179_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96324.1"
                     /translation="MKQKNRSLVVLLGLALTAIGGGCSRSVVSRTQESNWVTPPPATS
                     ISVTLNAPTGGGAGDIQGTRVVLISGGSILRDTTLASVTTAPFKFAFGNLAGVGTYQV
                     AIVKTGYTLQVTTASITGSAVPQVVFTLRKLENSNTISVASSLVTSAGTTVTIPQPPV
                     VTGTYSTAAPAQVTLPANSGISSVSVALQTASEVPTISTTQSVVSAVQIQTDATTTGT
                     VTVKFPLPLNSSDFAAAAGGTVDVLRLNLTTMTWVKIGVATIGADGTATYNVPGLTNT
                     NTVLCIGKTPSLSTAVQTVSTSTLLTPDQVANLVMSGVTTTTVPLNVSLGLGKASRSG
                     EVGGAAIDPPAGMDPAYWQMVLGVLMARLPELENYFKNGTQQVDYPLPKEQATLQKDR
                     RSYTFNFTYTWNTGVSKTFRIIVTYDHINIKPITHTSGVGG"
     gene            1907..>2428
                     /gene="ssb"
                     /locus_tag="P5179_13735"
     CDS             1907..>2428
                     /gene="ssb"
                     /locus_tag="P5179_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRS96325.1"
                     /translation="MASVNKVILIGNLGKDPVVDQTPSGTPRCRFSLATSENFTDREG
                     KKQERTEWHNILIWGKLAEVAGQYLRKGRPVYLEGRIAYRSYEDQEGNKKNFTEIVVT
                     SMQLLGSRSDQSGYDSDVPPPEEPLRDEKAEAFEFIALETSEITGRPFKEIHFPRGTI
                     VGAIVRGEEIIIPD"
BASE COUNT          545 a          667 c          736 g          480 t
ORIGIN      
        1 cgcttcacaa tacgccgtac gaagcgtgga ccagttacag ggtgaccgga ctcgatttgc
       61 gcattaccgc tcgatggggc agatatcttg tgggtgatcg tgcgtggcga ttggatgtcg
      121 agcgttcatt cggcgaaacg aagctcacgg ttttcggaat caaatcggac gggtactccg
      181 actatctgaa gcgagtagat cgtcagaata cgcggcttct gggcggggtg cggatggaaa
      241 tcccattgcc cccgcgtgaa cgagggatgc ctgaaagatt tcgcgtaaca tccactccag
      301 cgttcgcctg gtcctaccgg taccgagtgg gcgatgttgg tgtggacctg gtcacgaaac
      361 aatcagttga ggaccatgta ggggcttaca acccggtcgc tgtcctcaac aatcttgaac
      421 gcgcgcgaag tcaggtgcgc gcacggcaat cagaggcatg ccagtgagga agcacctttt
      481 ctccggtggg ggaacaatga aacaaaaaaa cagatcgctc gtggtccttc tggggctggc
      541 gctgacggca atcggcggtg gctgcagccg cagtgttgtc tcacgcacgc aggaaagtaa
      601 ctgggtgaca ccgccacctg ccacgtccat cagtgtgacg ctgaacgccc cgacaggtgg
      661 tggcgcaggg gacatccagg gaacgcgtgt cgttcttatt tcgggtggca gtattcttcg
      721 cgatacgacg ctggcctcgg tgacgacagc accgttcaaa ttcgcgttcg ggaacctggc
      781 cggggtggga acgtatcagg tcgcgatcgt gaaaaccggg tacacactcc aggtgacgac
      841 ggcttcgatc accggttccg ctgtgccgca ggttgtgttt accctccgga aactggaaaa
      901 ctctaacacg atctcagtgg caagctcgct ggttacaagt gccggcacaa cggtcactat
      961 cccccaacca ccggttgtga caggtactta ttctaccgcg gctccggcac aggtgacact
     1021 acccgcgaat tccgggatca gcagtgtttc ggttgcgttg cagaccgcgt cggaagtacc
     1081 tacgatcagc accacgcagt cggtggtaag cgcggtacaa atacaaacgg acgcgacaac
     1141 gacgggaact gtgacggtca agttcccgct tcccctgaat tcgtctgatt tcgccgcggc
     1201 agcaggcggc acggtggatg tgcttcgtct caaccttacg acgatgacct gggtgaaaat
     1261 cggcgtcgca accatcggcg ctgatgggac ggcgacgtac aacgttccag ggttgacgaa
     1321 cacgaatact gtcctttgca taggcaagac accatcgctg tccactgcgg tgcagaccgt
     1381 cagcacgtcg acgcttctca caccggatca agtcgcgaat ctggtgatga gcggcgtgac
     1441 aacgaccacg gtgccgttga atgtgtctct ggggcttggg aaagcgtcga ggagcggcga
     1501 agttggcggc gcggccattg atccgcctgc cggcatggac cccgcctact ggcaaatggt
     1561 gcttggggta ttgatggccc ggttgccgga gttggagaat tacttcaaaa acgggacgca
     1621 gcaggttgat tatcccttgc ccaaggaaca ggccacgttg cagaaggacc gcaggtcata
     1681 cacgttcaat ttcacgtaca cgtggaacac cggggtttcg aaaacgttca gaattatcgt
     1741 gacatacgac catatcaaca tcaagccgat cacccacact tcgggtgtgg gtggttgatt
     1801 tcgtctgaaa tggcccggat cgggatttgt tgatctttca cccgcgagcg agctgcccca
     1861 ccggctcgct cgcgttgttt caccaccaac catctgagga ggccccatgg cgagcgtgaa
     1921 caaagtgatt ctcatcggga acctcggcaa ggatccagtg gtcgatcaga cgccctcagg
     1981 gaccccacga tgccgcttct ccctcgcaac ctcggagaac ttcacggacc gagaaggcaa
     2041 gaaacaggag cggaccgagt ggcacaatat cttaatctgg ggaaaactgg cggaggttgc
     2101 gggtcagtat ctgcgaaagg gcaggccggt gtatctcgag gggaggattg cgtacaggag
     2161 ctacgaggat caggagggga acaagaagaa cttcaccgaa atcgtagtca cctccatgca
     2221 gcttcttgga tcgcgctctg accaatcagg atacgacagc gacgttccgc cgccggagga
     2281 gccgctgcgc gacgagaagg cggaggcctt cgaattcatc gcgctggaga catcggagat
     2341 caccggacga cctttcaagg aaatccattt tccgcggggc accattgtcg gcgccatcgt
     2401 ccggggggaa gagatcatca ttcccgac
//
LOCUS       DAOTNG010000401         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_34198_length_2412_cov_6.356385, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000401 DAOTNG010000000
VERSION     DAOTNG010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34198_length_2412_cov_6.356385"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2339
                     /locus_tag="P5179_13740"
     CDS             <1..2339
                     /locus_tag="P5179_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96326.1"
                     /translation="GGIVVGYVYKKEMTITPALRPYFADRAIDTKLTLTPTDRLKLRV
                     SYVYGTSCSAATSSLASSPELAETSAGGTFLDPTPLRDPGSLAGTVSGGRGSTNNKLS
                     LSNSCLLDGSYSQIGISISYTLRPTTFFSISLARSASETEQKRDLPRANVNASYDPAN
                     LASGYKPPSSFGYRGWLGMFYLWSDTGGTAAADLPSSLEDALTPGRMILNDPFSSPNY
                     SETVPSEGRFVTRTFFAGTSQPATVVSPQGYIQDGYADLSGTYTLGVGSESVMRANAV
                     QTMFKADFTHAWGAHTLKTGAEYATSELEYHARYALGLSVPGRNHSYMDYGGKYPTPR
                     PSILGVFVQDKFESQGLIANAGVRVERFDAHGRSFVPEIFTSQSFMRYGGKAIYDSIA
                     IAHGWDVSRWGGVPTYYPLRDTLRKYGLGDPPEPWDVMWALPYADNKVFWYVEPRFGV
                     SHPVTATTKFFFNYGIFCSVQKPAVLYGYQSDFHLGQTGRIQSARYASLRPAKTTMYE
                     VGIEHVMPFRMIATIRGYAKYNVDQVSNLQVSVGDGYSTYRNANYEEVRGIEMKIART
                     AGRFLNGWITLEEYATRTGEVGIVQFNTANIAQASYYTAYARSNEPLISLRGLVRIGT
                     PQEWGRLRGGWGISILETYREGSEVIYRPDPTIPIRELPPENYMKTIDYWFTNLKIDK
                     TMAVSRGRSMSLYADVTNLFNTKALSGAGEDYIAYVFQRRQNGETGLRVNDPSTFDAL
                     TRPYKDGGGNWKAPLKPRTEWLSFRNKRVVRIGLRFDL"
BASE COUNT          548 a          675 c          678 g          511 t
ORIGIN      
        1 tgggtggcat tgtggtgggg tatgtctaca agaaagagat gactatcacc ccggcgctcc
       61 gcccgtattt cgccgacagg gcgatagata ccaaacttac gctgaccccg acggacagac
      121 tgaagcttcg cgtctcgtac gtgtatggaa cgagttgctc agctgcaaca tcttcactcg
      181 cgagcagtcc ggagcttgcc gaaaccagcg cgggagggac ttttctggac ccaacgccat
      241 tgcgcgaccc ggggtcgttg gcaggaactg tctcaggcgg gagaggatca acaaacaaca
      301 aactcagcct ttccaattcc tgtctgctcg acggaagcta ctcccagatt ggaatctcga
      361 taagctacac gttgcgcccc acgacgttct tttccatctc gctggcacga tctgcgtcgg
      421 aaaccgaaca gaagcgcgac cttccccgtg ccaacgtaaa tgcgtcgtac gaccctgcga
      481 acctggcctc cgggtacaaa ccgccaagtt cgttcgggta ccggggctgg cttggaatgt
      541 tctacctctg gtccgatacc ggcggcactg ccgccgcgga tctgcccagc agccttgagg
      601 acgccctgac cccgggccgg atgattctga acgatccgtt ttcctcgccc aattattccg
      661 aaaccgtgcc ttcggagggg agattcgtta cccgcacgtt tttcgccgga acaagccagc
      721 ccgcgaccgt ggtgtccccg cagggataca ttcaggacgg atacgccgac ctgagcggca
      781 catacacact cggtgtcggc tctgaatctg ttatgagggc aaacgctgtt caaacgatgt
      841 tcaaggccga tttcactcac gcgtggggcg cgcacacgct gaaaacaggt gcggaatacg
      901 ccaccagcga actggaatac cacgcccggt acgcgcttgg tttgtcagtt cccgggcgga
      961 accacagcta catggactat ggcggcaagt accccacacc tcgacccagc atcctgggcg
     1021 ttttcgttca ggacaaattc gaatcgcagg ggctcatcgc caacgcgggc gtgcgtgtgg
     1081 aaaggttcga cgcacacggt cgttccttcg tacccgagat cttcacatcg caatcattca
     1141 tgcgctatgg agggaaggcc atttacgaca gcatcgccat cgctcacgga tgggatgtga
     1201 gccgatgggg cggagttccg acctattacc cgctccgcga caccctcagg aaatatggcc
     1261 ttggcgatcc ccctgaaccg tgggacgtca tgtgggcact gccttatgcc gacaacaaag
     1321 tgttctggta cgtggagcct cgtttcgggg tttctcatcc ggttaccgca actaccaaat
     1381 tcttcttcaa ttatggcatc ttctgttcgg tccagaagcc tgcggttctg tacgggtacc
     1441 aatcggattt tcacctgggg cagaccgggc gaattcaatc tgcgcgttac gcaagcctgc
     1501 gtccggcaaa aaccacgatg tatgaggtcg gcattgagca cgtgatgccc ttccgcatga
     1561 tcgctacgat tcgcgggtat gcgaaataca acgtcgacca ggtttccaat ctgcaggtga
     1621 gtgtcggaga tgggtattcg acatatcgca acgcgaacta cgaagaggtg cgcggcatcg
     1681 agatgaaaat tgctcgcact gcgggtcgtt tccttaacgg atggataacc cttgaggaat
     1741 atgcgactcg cacgggagag gtcggcatcg tgcagttcaa cacggccaac attgcgcagg
     1801 caagttacta cactgcatac gcacggtcga atgagcccct gatcagcctc cgcggattgg
     1861 tgcgcatcgg caccccgcag gaatggggcc gattgagagg ggggtggggc atcagcatcc
     1921 tggaaacgta ccgggaaggt tccgaagtga tttatcgccc ggatcctacc atccctattc
     1981 gagagctgcc accggaaaac tacatgaaga caatcgatta ctggtttacg aacctcaaga
     2041 tcgacaagac gatggctgtt agtcgtggaa ggagcatgtc actttacgct gatgtcacga
     2101 atcttttcaa caccaaagcc ctcagcggcg cgggggaaga ctatatcgcg tacgtgtttc
     2161 aacggcgcca gaatggtgaa accgggctcc gtgtgaatga cccttcgact ttcgacgcgt
     2221 tgacgcggcc ttacaaagac ggtggcggga actggaaagc gccactcaag ccgagaactg
     2281 aatggctttc ctttcgtaac aaacgcgtcg tcagaattgg actgagattt gatctttaac
     2341 aaactggcga atgaataggc tcagatggag aagatggtat gatcacgagg aatgcgtttt
     2401 tttgttgggc ag
//
LOCUS       DAOTNG010000402         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_34820_length_2380_cov_3.791398, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000402 DAOTNG010000000
VERSION     DAOTNG010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34820_length_2380_cov_3.791398"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..>2380)
                     /locus_tag="P5179_13745"
     CDS             complement(367..>2380)
                     /locus_tag="P5179_13745"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRS96327.1"
                     /translation="CTVRAERHLDSTTTDHKLLAPIRAAVPPVIDGILDDEVWEYAVQ
                     LDNFFVPALNRPPTERTILWLAFDDTCIYWAGRMFDSQPATLRMDQTRRNGYVLNDDY
                     ISIGFDVDNQHIGGGEYVFRMTPRGTQSEDFPDGSTSKVEWRGDWRGAARIDSLGWTV
                     EVAVPMRMFNRPGGPRIIGISSARRHPRTQERILWPNLGAQWDRSKCGDWPVVLPPFR
                     ERPRFMPYGVGEATRSSLDSYFGGDVKWMAPNGIKFTATANPDFQNVQNEILGLDFSY
                     TELIKGDNRPFFAEDVKFLPDSSVFYSNRVGKIYGGLKAVGNVGSDHRVGLINTYDRQ
                     EVNHLAGKWFWRPNGRVEASNAFAWRHGPFGTITKRNTPQARDNAVWVSSLGIDQLLG
                     RWTESYDFQGALSHTSDGVGDGYDVQASWDHSPTNTGFGLRLRGRLISEDFLMVDGGL
                     DTVEADQRYAAARFGYKRQYDRRAFKYWEVYANGAHASRFDGTLFQRSASLTGSLTIL
                     PGVNLDCAVEEKNRPPYNDRTGSLSFGWMQDRLFTAGKLSAVYGRVKDTDYFLLSASQ
                     GFRPIRALTAKVGAQYRRRDFPLGHVEDPAGGIEHLRQLIGTVQYDLTDERALSGRIV
                     YTNDGMNGYAAFQQMLRRGMDLFLIVGDPSADTWTERVAIKAVFVL"
BASE COUNT          399 a          718 c          804 g          459 t
ORIGIN      
        1 gggcgcagtc tggtcaggta acagcgttcg caggcagggg cgacgcatgc gtcgcgccct
       61 acggtgtttc atgtggttgt tttccttgcg gttacccact gaacatgata acacctgcga
      121 gcggccgtgc aggcaacccc acgcgttgcg ggtggggccc tgccccaact cagctttctt
      181 cgcgcacttg gcgttcttca cgtgcgcagt tgttccccgt gccctgaaaa aaccgtaccc
      241 cgatggaacc attttcccga aaacccttga cacgaagacg acgttttgcc ttagctaaca
      301 tacggggacg gaagctgcct tcggtgtgcg cgggcacgac aaagcgtgcc cctccattgc
      361 aggcacctac agcacaaaca cggctttgat agcgacgcgc tcggtccacg tatcggcgct
      421 ggggtcgccg acgatgagga acaggtccat gccgcggcgg agcatctgct ggaaggccgc
      481 gtagccgttc attccgtcgt tcgtatacac gatgcgcccg ctgagcgccc gttcgtcggt
      541 caggtcgtat tgaaccgtgc cgatgagctg gcgcagatgc tcgatgccgc ccgccgggtc
      601 ctccacgtgg ccgaggggga aatcgcgcct gcggtactgc gcgccaacct tggcagtgag
      661 cgccctgatg ggccgaaagc cctgactggc agagaggagg aagtaatcgg tgtccttcac
      721 ccgcccgtag acggcggata gtttcccggc ggtgaacaag cgatcctgca tccagccgaa
      781 ggacaggctg ccggttctgt cgttatatgg cgggcggttc ttctcctcaa cggcgcagtc
      841 gaggttcaca ccgggaagga tcgtcaggga gccggtgagg ctggcggagc gctggaacag
      901 ggtgccgtcg aaccgtgagg catgcgcgcc gttggcgtag acctcccagt atttgaaggc
      961 cctgcgatcg tactggcgtt tgtagccgaa ccgcgcggcg gcgtaccgct ggtcggcttc
     1021 cacagtgtca aggccgccgt ccaccatgag gaagtcttcg ctgatcaggc ggcccctgag
     1081 gcgcagtccg aagccggtgt tggtcgggct gtggtcccag cttgcctgca cgtcgtagcc
     1141 gtcgcccacg ccgtcgctgg tgtgcgagag cgcgccctgg aagtcatagc tttccgtcca
     1201 gcggccgagc aactggtcaa tgccgaggct ggagacccat acggcgttgt ctctcgcctg
     1261 cggggtattt cgtttcgtga tggtgccgaa aggcccgtgt cgccaggcaa atgcgttgct
     1321 ggcttccacc cgcccgtttg gccgccagaa ccatttgccg gcaaggtggt tcacctcctg
     1381 ccggtcgtag gtgttgatga gccctacgcg gtggtcgctg ccgacgttgc cgaccgcctt
     1441 caggccgccg taaatcttcc cgacacggtt gctgtagaag acggaagaat cgggcaggaa
     1501 cttgacatcc tccgcgaaaa atggccggtt atcgcctttg atgagttccg tgtaggagaa
     1561 gtcgaggccg aggatctcgt tctgcacgtt ctgaaagtcg gggtttgccg tggcggtgaa
     1621 tttgatgccg ttgggggcca tccacttcac gtcgccgccg aagtaggaat cgagcgagga
     1681 tcgcgtcgcc tcgcccacgc cgtagggcat gaaccggggg cgttcacgga atggcggcag
     1741 cacgaccggc cagtcgccgc atttgcttcg atcccactgg gcgccgaggt tcggccagag
     1801 gatgcgctcc tgcgtgcggg gatgacgccg cgcggaggag atgccgatga tgcgggggcc
     1861 gccggggcgg ttgaacatgc gcatcggaac agccacctcg accgtccaac cgagggagtc
     1921 tatgcgggcc gcgccccgcc agtcgccgcg ccattccacc ttcgatgtgg agccgtccgg
     1981 gaagtcctcc gattgcgtgc cgcgcggggt catgcggaat acgtattcgc cgccgccgat
     2041 gtgctggttg tccacgtcga acccgatgga gatgtagtcg tcgttcagca catacccgtt
     2101 tcgccgggtc tgatccatgc gcagcgtggc cggttggctg tcgaacatcc gcccggccca
     2161 gtagatgcag gtgtcgtcga atgcgagcca gaggatcgtg cgctcggtgg gggggcggtt
     2221 gagcgccggc acgaagaagt tgtcgagctg gacggcgtac tcccagacct cgtcgtcaag
     2281 gatgccgtca atgacgggcg gcacggcggc gcggattggt gccagcagct tgtggtcggt
     2341 ggtggtggaa tccaggtggc gctcggcgcg gacggtacac
//
LOCUS       DAOTNG010000403         2363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35147_length_2363_cov_4.934575, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000403 DAOTNG010000000
VERSION     DAOTNG010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35147_length_2363_cov_4.934575"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1220)
                     /locus_tag="P5179_13750"
     CDS             complement(51..1220)
                     /locus_tag="P5179_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS96328.1"
                     /translation="MNTRSLFLGAAALAGLLSIPAAGGRTAPAIEGTVVLASDSLARV
                     RGIDVRLVAFSDTGVSLVATGTSDAQGRFRFPAVPADSSVYYRMEGLYAGVWQPGEPF
                     VTPPVGARLAVFDTTSSATRIAVEKLHLILNPLESGIAVTAIYILRNDGPIYLGAPQA
                     AHDRPRVALEFFLPSGAEDLQVFDGSLAGHRVPTERGFGWLVPFYPGIDTLVFGYTLP
                     WDGKRLRFPVESPYPVSSLSVITMQGTAQLSVEGAERVSSEEAPDLVAYGRRAVPAGT
                     PVMVEVRAPGVGRFPLSWGIVIALGVALIGGAAAIAWRRRTVRPAQTRSTEAGGNGGA
                     TTPDSTVDALVDEIARLDEEHETGSIPEAEYANKRRELKEKLVQALRQPGEPAQP"
     gene            complement(1217..1660)
                     /locus_tag="P5179_13755"
     CDS             complement(1217..1660)
                     /locus_tag="P5179_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRS96329.1"
                     /translation="MLAALVVVVIALAVGAYVGYPLLFPGRNPQEEDPGRHEFELRSA
                     QIAGALRELETDHSPGKVADDDFAERRARLAREFESVEQRLAGAAPSDQTDLDELAEK
                     LVAARRAARTHPRPGACPRCGQSNPPAARFCMNCGSRLEEREPTS"
     gene            complement(1667..>2363)
                     /locus_tag="P5179_13760"
     CDS             complement(1667..>2363)
                     /locus_tag="P5179_13760"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRS96330.1"
                     /translation="LGIVVAAGLAFAGMRNGYALAFFAICAFVLGTIVQEVLRTFRTR
                     AATLDSPPARKAMVAALTPNRRFGAYIVHFGVIIVAVGITGSSLFERKTDLVLRQNEV
                     KPFEDQAFRYMSFRQTREPNRDVYAAGVEVTRAGEVRAKVEVVKEDYHAEQMRWTKAR
                     ILSSVAEDVYLTLAAFQEDGSAITLSVRRIPLVVWLWVGGAVLVLGSLIAMLPDARER
                     VRRTRPASETGER"
BASE COUNT          418 a          784 c          754 g          407 t
ORIGIN      
        1 ctattttccg ggagacagga tgtagcgcgg gcggacacag aggtccgccc ctacggttgc
       61 gcaggctcgc cgggctggcg cagcgcctgc accagcttct ccttgagttc cctccgcttg
      121 ttcgcatact ccgcttcagg gatcgatccc gtctcgtgct cttcgtcgag gcgggcgatt
      181 tcgtccacca gcgcgtccac agtgctatcc ggcgtggttg cacccccgtt gccgccggct
      241 tctgtggacc gggtttgtgc cgggcgcacc gttcggcggc gccatgcgat cgccgcggca
      301 ccaccgatca gcgcgacccc gagggcgatc acgatgcccc acgaaagggg gaaccgcccc
      361 acaccgggtg cgcgaacctc caccatcacc ggcgtcccgg caggcaccgc acgccggccg
      421 tacgcaacga gatcaggtgc ttcttccgac gagacccgtt ccgctccctc gacggaaagc
      481 tgggctgtac cctgcatcgt gatgacgctg agcgaagaga ccgggtaggg tgattccacg
      541 gggaaccgca ggcgctttcc atcccacgga agcgtgtatc cgaacacgag cgtgtcgatc
      601 ccggggtaga acggaaccag ccatccgaag ccgcgttcag tcggcacgcg gtggcccgcc
      661 agcgatccat cgaacacctg caggtcttcg gctcccgaag ggaggaagaa ctcgagtgcc
      721 acacgcgggc ggtcgtgcgc ggcctgaggt gcgccgaggt agatcggccc gtcgttgcgc
      781 aggatgtaga tggccgtcac ggcgatgccc gattccagtg gattgaggat gaggtgaagt
      841 ttttccaccg cgatgcgggt agctgaggag gtcgtgtcga aaacggcgag gcgcgcaccg
      901 acgggtggcg tcacaaatgg ttctccgggc tgccagacgc ccgcgtaaag cccctccatg
      961 cggtagtaca cactggaatc cgccggcacg gcgggaaagc ggaaacgccc ctgcgcgtca
     1021 ctcgtgccgg ttgccacaag ggagacgccg gtatcactga acgccacgag cctcacgtca
     1081 atgccgcgga cacgggcaag ggaatccgag gccagaacaa ccgtgccttc gatcgccggt
     1141 gcggtgcgtc cgccggccgc cgggatcgac aacaggccgg cgagtgcggc ggctccgagg
     1201 aacagggatc gcgtgttcat gacgtcggct cccgctcttc aagtcggctg ccgcagttca
     1261 tacagaagcg ggcggcgggt gggtttgatt gcccacaacg ggggcatgct ccgggccggg
     1321 ggtgtgttct ggccgctctg cgtgctgcca caagtttttc ggctagctcg tccaggtctg
     1381 tctggtctga tggcgccgcg ccggccagcc gctgctcgac ggattcgaac tcacgcgcga
     1441 gtcgggcgcg tcgttccgcg aagtcgtcgt ccgcaacctt gcccggtgag tggtctgttt
     1501 cgagttcccg cagagcgccg gcaatttgcg cgctgcgcag ttcgaactca tggcgtccgg
     1561 ggtcttcttc ctgcgggttc cggcccggaa acagaagcgg gtagcccacg tatgccccca
     1621 cggccagggc gattacgacc acgacgagcg ctgcaagcat ctgcgcctac ctttcgccgg
     1681 tttctgacgc cgggcgggta cgtcgaaccc gttcccgcgc gtcgggcagc atggcaatca
     1741 gacttcccag cacgagcacg gcaccgccga cccacagcca caccacgagc ggtatccgtc
     1801 gcacggagag agtgattgcg cttccgtctt cctgaaatgc ggccaacgtg aggtacacgt
     1861 cctccgccac gctggaaagg atgcgggctt tcgtccagcg catctgctcc gcgtggtaat
     1921 cctctttcac gacttccacc ttcgcgcgca cctcgcccgc ccgtgtgact tcaacccccg
     1981 ccgcatagac atcgcggttt ggctcgcggg tctggcgaaa tgacatgtag cggaacgcct
     2041 ggtcctcaaa tggcttcact tcgttctgtc gaagcaccag atcggtcttg cgctcgaaca
     2101 gtgaggaacc ggtgattcca accgcaacaa tgatgacgcc gaaatgcacg atgtacgcgc
     2161 caaaacggcg attcggggta agcgccgcca ccatcgcctt ccgggcgggt ggggaatcga
     2221 gtgttgccgc gcgcgtgcgg aaggtccgga gcacttcctg cacgatcgtg cccagcacga
     2281 acgcacagat cgcgaaaaac gccagcgcgt atccattgcg catgcctgca aaagcaaggc
     2341 ctgccgccac cacaattccc agc
//
LOCUS       DAOTNG010000404         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35300_length_2356_cov_3.588874, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000404 DAOTNG010000000
VERSION     DAOTNG010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35300_length_2356_cov_3.588874"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="P5179_13765"
     CDS             <1..890
                     /locus_tag="P5179_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96331.1"
                     /translation="RPTWNDLELHAVDLHIHAGTESPEDYRLHDLVSYFVATGRRVMC
                     LTDHWKRFLFPSKKPLTHYPGTVDGWRQFAADVFREREKFPDHLLLFGPEIHLGGMMQ
                     DVTTPLLETPEVDLFLGEAGVAPEGMKYGDYLVQGVEEIAQCRDRYRLPGFLAHPIRD
                     IINRICGKTGPGPAHPKHPQFPPLDALADPLAHTEEILDIDIERLARTCVRLDVPIEI
                     NESDFSRIMGQNHESFMERYLLFNRTMIDAGVSVVLGSDMHSVESATCTPFVPARMLG
                     VKPRDMRFLEHWLGPRPPD"
     gene            969..1862
                     /locus_tag="P5179_13770"
     CDS             969..1862
                     /locus_tag="P5179_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96332.1"
                     /translation="MRPTRNDLELYAIDLHIHAGTERPEAYSLLDLVSYIVATGRRIV
                     CLTDHSGRFLGLSRKPLAHYEGSYDGWRRFAADVQEARERFPGTIILFGPETGFGGLT
                     SDTVAPLFETPGVDLFLGEPGAAPEGVSYGDYLIAGVEDIARCRDRYKIPGFLVHPLR
                     DVINRICGKTGPGPAHPKHPPFPPLGSFADPRAHTEEVLGVDIARLAQTCIRLDVPVE
                     INESDFGRILGQNHQSFLDRYFFFYRSLIDAGVSVVPGSDMHNVEHPACTPFFPATIL
                     GVKPQDIRFLEQWLGQCGRGT"
     gene            complement(1953..>2356)
                     /locus_tag="P5179_13775"
     CDS             complement(1953..>2356)
                     /locus_tag="P5179_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96333.1"
                     /translation="PNGVPRRELPPEYVLRGRDYWGTSAKFTKDALVGRNRRVSVYLD
                     ITNLFNRKYLNGTYMNSGDYVAYIVERRAQGEPDLRVGDPSTWHVLTEPYRIRKADGT
                     YTAWKPPISPRTEWLAFLYPRAYRAGIRFDL"
BASE COUNT          446 a          753 c          700 g          457 t
ORIGIN      
        1 tgcgccccac atggaacgac ctcgaactcc acgcagttga cctgcacatc cacgccggca
       61 ccgaaagccc ggaagactac cgcctgcacg atctcgtctc gtatttcgtg gcaaccggtc
      121 gccgggtcat gtgccttacc gaccactgga agcgtttcct tttcccgtcg aagaaaccgc
      181 tcacgcatta ccccggcacg gtggacgggt ggcgccaatt cgctgctgat gtcttcaggg
      241 agcgcgaaaa gttccccgac cacctgctcc tcttcggccc ggagatccac cttggcggca
      301 tgatgcagga cgtgacgacc cccttgctcg agacaccgga ggtggacctg tttctcggcg
      361 aagccggcgt ggcgccggag gggatgaaat acggggatta tctcgttcag ggggtggagg
      421 aaattgcgca atgcagggac cgttaccgcc tccccggctt tcttgcgcac ccgatccggg
      481 acatcatcaa tcgaatctgt ggcaaaacgg gtcccggccc ggcccatccg aagcacccgc
      541 agttcccgcc cctggatgcg ctggctgatc cgctcgcgca tacggaggaa atcctcgata
      601 tcgacatcga gcgactggcg aggacctgcg taaggttgga cgtccccatc gagatcaacg
      661 agtcagattt cagccgaata atggggcaga accacgagtc gttcatggag cggtacctgc
      721 ttttcaacag gacaatgatt gatgccgggg tgtccgtcgt gctgggcagc gacatgcaca
      781 gtgtggaaag cgccacgtgc accccctttg tgccggcaag gatgctgggt gtgaaaccgc
      841 gcgacatgcg cttcctcgag cactggctcg ggccgcgtcc gccggattga ggtgcgtcgg
      901 aaggcctgcg aggatacctt tgatagcccc cttctgccaa ctgccacaga ctaccgaaag
      961 ggctccattt gcgtcccaca cggaatgacc tcgaactcta cgcgattgac ctgcacatcc
     1021 acgcgggcac cgagcgcccg gaagcgtaca gtctcctcga tctcgtctcc tacatagtgg
     1081 caacgggacg gcgtatcgtg tgcctgaccg accactccgg tcgtttcctg ggtctttctc
     1141 ggaagccctt ggcgcattat gaagggtcgt acgacggatg gcgcaggttc gccgcggacg
     1201 ttcaggaagc gcgcgaacga ttccccggca ccatcatcct gttcgggccc gaaacgggct
     1261 ttggcgggct cacttccgat acggtcgcgc ccctgtttga aacgccggga gtggacctgt
     1321 tcctcggtga accgggcgcg gccccggaag gcgtgagcta cggcgactat ctcatcgcgg
     1381 gagtagagga catcgcgcgg tgcagagatc ggtacaaaat ccccggattt cttgtccatc
     1441 cccttcgcga cgtcatcaac cggatttgcg gcaaaaccgg tccgggcccg gcacacccga
     1501 agcacccgcc gttcccgccc ctcgggtcgt ttgccgaccc acgcgcccac acggaggaag
     1561 tcctgggagt ggatatcgcc cgactcgcgc aaacctgcat ccggctcgat gtgccggtgg
     1621 aaatcaacga gtcggatttc ggccgcatcc tcggccagaa ccaccagtcg ttcctcgacc
     1681 ggtatttttt cttttacagg tcattgatcg atgcgggcgt ctccgtcgtg ccgggcagcg
     1741 acatgcacaa cgtcgaacac cctgcctgca cgccgttctt ccccgcaacc atcctcggcg
     1801 tcaaaccgca ggatatccgc tttctggaac aatggctggg gcagtgcggg cgaggaacct
     1861 gaggaacttg gggcgtacgt cagcgcagca gaaaggcgcc cggattgtcg ccgggcgctt
     1921 ttcctttctg cgcaacttat gtgctcgctc ggttacagat caaagcgaat cccggcccgg
     1981 taggcacgcg gatacaggaa cgccagccac tccgtgcgcg ggctgattgg cggcttccag
     2041 gcagtgtacg tgccatccgc ctttcggata cgatagggtt cggtcagcac gtgccacgtg
     2101 gagggatcac cgacgcgcag atcgggctct ccctgcgcgc gtcgttccac gatgtacgcc
     2161 acgtaatcgc cgctgttcat gtaggtgccg ttgagatatt tccggttgaa aagattcgtg
     2221 atgtcaagat acacagacac tcgcctgttg cggccgacca acgcgtcttt ggtgaacttc
     2281 gcgctggtgc cccagtaatc acgcccccgg aggacatact ccggaggaag ctcgcgcctc
     2341 ggcacaccgt tcgggt
//
LOCUS       DAOTNG010000405         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35473_length_2348_cov_3.805059, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000405 DAOTNG010000000
VERSION     DAOTNG010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35473_length_2348_cov_3.805059"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..2106)
                     /locus_tag="P5179_13780"
     CDS             complement(103..2106)
                     /locus_tag="P5179_13780"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS96334.1"
                     /translation="MARTQSPDVTKADMQQCLDWTGGFFASPAALPVSYTYGGKGYRG
                     IPAEFAPKIRTRRIDACIIATSIEGRDRATGLLVRAEIVRYLDFPVVEWTVYFTNEGE
                     KDTPILSDIQGLDADFAGTTPVLWHCNGDYYSIDGYTPFETPLNEGASVTIAPNGGRS
                     CNGAFPYFRVMTADAVLTFAVGWPAQWSATFSGTATGAHITAGQQLTRMKLLPGETMR
                     TPRISLLAVTGDMQRSINVWRRWYQAHVLPRSDGQPLPQKLVAHAVPFGDEFTEATEE
                     NQIAYMEKFAQKGYKFSTWWIDAGWYPCRMPNGKGDWPTTGTWIPDPPRFPNGFKPVS
                     DAAHKHGADLLIWFEPERVRKGTQIDREHPEWLLALKSNPDNNDKLLNLGNPACRQWL
                     TDHVSKLISDGGIDIYRQDHNFDPLEFWRQNDAEDRQGANENLHVQGYLQYWDDLLAR
                     NPGLWIDSCASGGRRNDLETMRRSVPLHYTDFGYGIHYVKLSFHHTLYQWIPYIKECT
                     LSWDKDYTTFQRFEEQIDRFAFHCGIAAMTTPAVDINRDDYDYAQAAKLTELAFKTGE
                     YFIHGDYYPLTPFARAEDKWVVRQFHRAADGTGFVQGIRLQKCPEESITVFPQGLDDT
                     LRYVFEDAETGEKREVSGAALRVTGFTLKQPVRSGAVWLYRPV"
BASE COUNT          387 a          731 c          772 g          458 t
ORIGIN      
        1 acttgccgcc ctgcgggcag caagctgccc gggggcagca ggctgcccgg gggcagctcg
       61 ccactcgacg acctcgccgg gctcacaccg gcctgaccgg ccttacacgg gccggtaaag
      121 ccacaccgcg ccgctgcgga cgggttgctt caacgtgaat cccgtgactc tgagcgccgc
      181 gccggagacc tcgcgttttt ccccggtttc cgcatcctcg aacacgtaac ggagggtgtc
      241 gtccaacccc tgcgggaaga cagtgatgct ttcctccggg cacttctgca accggatgcc
      301 ctgcacgaac ccggtgccgt ccgccgcgcg atggaactgg cgcacgaccc acttgtcttc
      361 tgcgcgcgca aacggggtga gcgggtagta gtcgccgtgg atgaagtact cgcccgtttt
      421 gaacgcaagc tccgtcagct tcgccgcctg cgcgtagtcg tagtcgtccc ggttgatatc
      481 cactgccggc gtcgtcatgg cggcgatgcc gcagtggaac gcgaaccggt caatctgctc
      541 ctcgaaccgc tggaacgtgg tgtagtcctt atcccacgag agcgtgcatt ccttgatgta
      601 ggggatccac tgatacagcg tgtggtggaa ggagagcttg acgtagtgga tgccgtagcc
      661 gaagtcggtg tagtgcagcg gcaccgaccg gcgcatggtt tcgaggtcgt tccggcgtcc
      721 gcctgaggcg caggagtcta tccacaggcc ggggttgcgg gcgaggaggt cgtcccagta
      781 ctgcaggtag ccctgcacgt ggaggttctc gttggcgccc tgccggtcct cggcgtcgtt
      841 ctggcgccag aactccagcg gatcgaagtt gtggtcctgc cggtagatgt cgatcccgcc
      901 gtcgctgatg agcttgctga cgtgatccgt cagccactgg cgacaggcgg ggttgccgag
      961 gttgagcagc ttgtcgttgt tgtccgggtt gctcttgagc gcgaggagcc attcggggtg
     1021 ctctctgtca atctgcgtgc ccttccggac gcgctccggc tcgaaccaga tgagcaggtc
     1081 cgcgccgtgc ttgtgcgcgg cgtcggagac cggtttgaac ccgttcggga agcgcggcgg
     1141 atcgggaatc catgtgccgg tggtgggcca atcgcctttg ccgttcggca tgcggcaggg
     1201 gtaccaaccc gcatcaatcc accaggtgct gaacttgtat cccttctggg cgaacttctc
     1261 catgtaggcg atctggttct cttccgtcgc ctcggtgaac tcatcgccga acggcaccgc
     1321 gtgagccacc agcttctgcg ggagcggctg cccatccgag cgcgggagca cgtgcgcctg
     1381 gtaccagcgc cgccagacgt tgatcgagcg ctgcatatcg ccggttacgg cgagcaggga
     1441 aatgcgcggc gtgcgcatgg tttcgccggg gaggagcttc atgcgggtga gctgctgccc
     1501 cgccgtgatg tgcgcgcccg tggcggtgcc cgagaacgtc gcgctccact gcgcgggcca
     1561 gcctaccgcg aaagtaagta cagcatccgc cgtcatcacg cggaaatagg ggaacgcgcc
     1621 gttgcacgag cgcccgccgt tcggggcaat agtaacgctg gcgccctcgt tcagcggcgt
     1681 ttcaaacgga gtgtagccgt caatgctgta gtagtcgccg ttgcagtgcc agagcaccgg
     1741 ggtcgtgccg gcgaagtcgg cgtcgagccc ctgtatgtcg ctcaggatgg gcgtgtcctt
     1801 ctcgccttcg ttcgtgaagt agaccgtcca ctccacgacc gggaagtcga ggtagcgcac
     1861 tatttccgcc cgcaccaaga ggccggttgc gcggtcgcgc ccctcgatgg aggtcgcgat
     1921 gatgcaggcg tctatccggc gggtgcggat ttttggcgcg aattccgccg ggataccacg
     1981 gtaccccttg ccgccatagg tgtaggaaac cggcagcgcc gccgggctgg cgaaaaagcc
     2041 gccggtccag tccaggcact gctgcatatc ggcttttgtt acatccggag attgcgttct
     2101 ggccatctga cacctctctc aaagcaggga aagcgggaga ctgtacaacg aagagagcct
     2161 tctgtaagga taatccagaa ggctcttggg ggctattggg gcattgcgga cggcgtgaat
     2221 tcctacctca ccagaaccat gcgcttcgtt gaggcgcccc gcgatgtcgt gactgtgtac
     2281 agatacacac cgctggcggc gtctcttccc agagcatctt gcccgtccca cacaacggaa
     2341 tgcatccc
//
LOCUS       DAOTNG010000406         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35589_length_2342_cov_4.414954, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000406 DAOTNG010000000
VERSION     DAOTNG010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35589_length_2342_cov_4.414954"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5179_13785"
     CDS             <1..462
                     /locus_tag="P5179_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRS96335.1"
                     /translation="NWVYVDRRTGRPRRLEREILDAFQQKSEYALEDRDWGAAVLSRT
                     YPADPQFTTTHVVRWTELDAAGHVNNAVYADWIRDDCAAFRPEARIVRLRLNYRQPAL
                     LGESLVRELVPLEETVWGYRIRRCESEETISLAVIQTTLSVVPNEECLRDG"
     gene            complement(407..1390)
                     /locus_tag="P5179_13790"
     CDS             complement(407..1390)
                     /locus_tag="P5179_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRS96336.1"
                     /translation="MSAPGRGENICLNMRDLLSAYLDGETTPEETARVDAHLNTCEAC
                     RVVLARYRTIERAIAGEVPGADPAPQDSAVFTDAVMSRIREVADRPTLKAHILKWRTP
                     VVSALAIAATVVLALYLRPRTPEEPIHPVPTTTVPSPQERRETAQIASKPSERRPLTS
                     GGAPTPRTRAQIAEPPVVASAELGQAMGDIAPDETSIQDAKAKPARGGVSFVQDFYAT
                     RAAQAITGAAPAKSDSVVPDLRRKADAMRTELVSANETNSVDSLKLLLGDVLARIAER
                     TGEPLDVRAALTFWRQEYAVLAKQSSDSVVQSRVSRLEGILRSAQPKGSFE"
     gene            complement(1395..2018)
                     /locus_tag="P5179_13795"
     CDS             complement(1395..2018)
                     /locus_tag="P5179_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS96337.1"
                     /translation="MISLAGVTAVLVVIAALRSLEAASDQEIVSGVLAGNTDYFEGLV
                     VRYQRQVFRIALRVTRDRDNAADVAQEAFVRAFVALKQYDANRPFGAWISRITLNTAL
                     TLCSSRKKQGDERRTATLDNSNAADAFSTVARTEFETAVRAAIAGLPDGIRETFELRA
                     YADLSYDEIAHELGVPRGTVMSRLARARERLREVLEQQGFGRESEAV"
BASE COUNT          447 a          709 c          702 g          484 t
ORIGIN      
        1 aactgggtgt acgtggatag aagaaccggc cgtccgcgcc gactcgagcg ggaaatcctt
       61 gacgcattcc aacagaaaag cgagtatgcc ctcgaggatc gtgattgggg tgcggcagtt
      121 ctttcccgta cgtaccctgc cgatccgcag ttcaccacca cccacgttgt acggtggacg
      181 gaacttgacg cggccggcca cgtgaacaac gccgtgtacg cagactggat acgggatgac
      241 tgcgcggcgt ttcgtcccga ggcacgaatc gtacgcctcc gcctgaacta ccggcaacct
      301 gcgcttctgg gggaatcgct ggtgcgggaa cttgtgcccc tggaagaaac tgtgtgggga
      361 taccgcatcc gccgctgcga gtccgaggaa acaatcagtc tcgcggtcat tcaaacgacc
      421 ctttcggttg tgccgaacga agaatgcctt cgagacggct gacgcgagac tgaactacgg
      481 aatcgctgct ctgtttcgcc agaacggcgt attcctgacg ccagaacgtg agcgcggcgc
      541 gtacatcaag cggttcgccg gttctttccg ctatccgcgc gagaacatcg cccagcagga
      601 gtttgagcga atccacggaa ttcgtctcgt tggccgaaac gagctcggtt cgcatcgcgt
      661 ccgctttcct cctcagatcc ggcacaacag agtcgctttt cgcgggggcg gcgcccgtga
      721 tcgcctgtgc cgcacgggtt gcatagaaat cctgaacaaa actcacgccc ccgcgggccg
      781 gtttcgcttt tgcgtcctga atggatgtct catccggggc gatgtcgccc atcgcctggc
      841 caagctccgc ggaagccacc acaggcggtt ctgcaatctg tgcccgcgtg cggggggtgg
      901 gcgccccgcc tgatgtgagc ggcctccgct cggaaggttt ggatgcaatt tgcgcggtct
      961 cccgccgttc ctgaggggag ggaactgttg tagtgggcac cggatgaatc ggttcctctg
     1021 gggtccgcgg gcggaggtag agcgccagta ccacagtggc cgcgatcgcg agcgctgaaa
     1081 ctacgggagt gcgccatttg aggatgtgag ccttgagggt cggcctgtct gccacttcgc
     1141 gtatccgcga catgacagcg tccgtgaaca ccgccgaatc ctgcggagcg ggatcagcac
     1201 cgggaacctc cccggcgatc gcgcgctcaa tggtgcggta gcgagcgagc acaacccggc
     1261 atgcctcgca cgtgttgagg tgcgcgtcta ctcgtgcggt ttcctccgga gtggtctcgc
     1321 cgtcgaggta cgcgctcaaa aggtcgcgca tgttcaggca gatattctcg cctctcccgg
     1381 gggcactcat ggttctagac cgcctcgctc tctctgccga aaccttgttg ttcgagtact
     1441 tcgcggagac gctctcttgc gcgtgccagc cttgacatca ccgtaccccg tggaacgccc
     1501 agctcgtgcg cgatctcatc ataactgagg tccgcgtacg cccggagttc aaaggtttcg
     1561 cgtattccgt ccggcagacc cgcgattgcc gctcgaaccg ctgtttcgaa ctcggtcctc
     1621 gcaacggtgg aaaacgcgtc cgccgcattt gaattgtcca gggtggctgt tctccgctca
     1681 tcgccctgtt tctttcggct gctgcaaagc gtcaaggccg tattgagtgt tatgcgtgag
     1741 atccatgcgc cgaaagggcg gtttgcgtcg tattgcttca atgccacgaa agcccgcacg
     1801 aacgcctcct gagcgacatc cgccgcatta tcgcggtccc tggtcacgcg cagcgcgatt
     1861 cgaaacacct gccgctgata ccggacaacc aatccttcaa agtagtcggt attgccggcg
     1921 agaacgcctg aaacaatctc ctgatccgag gccgcttcca ggctgcgcaa ggctgcaatc
     1981 acgacaagaa cggccgtcac gccagcgagg ctgatcacgg gtttcgttac tccagattac
     2041 tttcgccgcg cgcggaatat tcccgtcact gcggctgaaa agaaattgac cgtaagaaat
     2101 gacgttgtgt cggaaaagat acggaattag cttcacggag tggtgtcgga caacagacga
     2161 tgttttttcc ttggaggctc ttccatggcg cacactatcg ccgttcttcc tcagaaggat
     2221 cttcgaaacg cgctcttcag tgaaaaggcg tggaaggaac tgggaacact gggacgcgta
     2281 acgtacgaac cggacgcgga ccggcctcct gatgattccc gtgttcgggc tcttcttgcg
     2341 ga
//
LOCUS       DAOTNG010000407         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35668_length_2338_cov_3.689444, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000407 DAOTNG010000000
VERSION     DAOTNG010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35668_length_2338_cov_3.689444"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5179_13800"
     CDS             <1..965
                     /locus_tag="P5179_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HRS96338.1"
                     /translation="LCHPDFQENDGRLWLTDAEDAWFVRVDHITKYGTDHDLEQMTIA
                     PARPLLFLSIDAVPNGTALIWDHVEDAPGVPCPNPRVILPRDIVPGVVEGRVPVDIRS
                     FGVRTPPCTAAAPSYGIIGLFHILPPALAWLWRLASPRGYHNPSVVQAEQMGSEGVGS
                     YWPFATGLRVEQANLLLEQFVRNRRMRHVLVPNQHIGVWRTGFMPQWVARDYLARRGH
                     AKFQSDQCRPARCPLLGYALQSIRIEGTTIPGWLFQVDQQPEVGTEAYDEGARILTEF
                     FRRQVQKFLKNNLDELGRKIIDCFLANGTVEDYEKLLPVSDGVY"
     gene            complement(1017..2258)
                     /locus_tag="P5179_13805"
     CDS             complement(1017..2258)
                     /locus_tag="P5179_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRS96339.1"
                     /translation="MSDSTITPATRWKVAIAAVAIHLCIGSLYAWSVFIKPLQAAHGW
                     SRPELATAFSLAFLTGGLFSAFRGAWMECRGPRRCAVMSAVCFGSGIAGAGIAAHMGS
                     LWVLYATYGILGGFGLGMGYVPPINALLKWFPDRRGLAGGMAVAGFGAAALVTGPAAT
                     KLVSLVEVPLTFAILGAAYFVVILLASCAMRFPPADYRPAGWTPRSETAGGLQEKTHT
                     LGEALKSPRFWLLWVVFFLNISAGMSLISFGAPMAQEIIGMTAVQASVMVGFMGVFNA
                     AGRIGWSALSDYIGRPVTFAVMVGIQIVLFALVPSVSSVALFEAAFFVIMTCFGGGFA
                     TCPAFVTELFGSRNTGAIYGAILTAWSAAGVAGPTMAAMLRDLTGSYSGGMYVIAGAL
                     AIALVCVAALGASIRGRPAAV"
BASE COUNT          506 a          729 c          727 g          376 t
ORIGIN      
        1 gtctctgcca ccccgatttt caggagaacg acgggcggct ctggttgact gacgcggaag
       61 acgcgtggtt tgtgcgcgtg gatcacatca cgaagtatgg cacggaccac gacctcgaac
      121 agatgacgat cgcgccggcg cgcccgcttc tcttcctcag cattgacgcc gttccgaatg
      181 gaactgcgct gatctgggac catgttgagg acgcacccgg tgtgccgtgt ccgaatccac
      241 gggtcattct gccgagagac atcgtccccg gcgttgtcga ggggcgcgtg ccggtggaca
      301 tccgaagttt cggcgtacgg acaccgccct gcaccgcagc agcgccgtcg tacggaatca
      361 tcgggctgtt ccacatcctc ccgccggctc tggcgtggct ctggcggctt gcttcaccaa
      421 ggggatatca caacccgagc gtcgttcagg cagagcagat gggaagcgaa ggggtgggaa
      481 gctactggcc gttcgcaacc ggtctgcggg tggaacaggc gaacctgctg ctcgagcagt
      541 ttgtcaggaa cagaaggatg cggcacgtac tggtaccgaa ccagcacatc ggcgtgtggc
      601 ggacaggatt catgccgcag tgggtcgcgc gcgattacct cgcccgccgg ggtcacgcga
      661 agttccagtc ggaccagtgt cgtcctgcca gatgccctct actcggatat gccctccaga
      721 gtattcgtat cgagggcacc acgatccccg gatggctgtt tcaggttgat cagcaaccgg
      781 aggtcggcac ggaggcgtac gacgaagggg cgaggattct tacggagttc ttccgaaggc
      841 aggttcagaa gttcctgaag aacaatctgg atgaacttgg tcgaaagatc atagactgtt
      901 tcctcgccaa cggcacggtg gaagattacg agaagctcct cccggtttcc gacggcgtat
      961 actgatcggc gcccgcgtga cgtgaacgtc gtgttgacaa gtgcgccgaa gtgccttcag
     1021 actgcggcgg ggcggccacg aatggaagca cccagcgcgg cgacgcacac cagcgctatg
     1081 gcaagggcac ctgcgatcac gtacatgccg cccgaataac tccccgtcag gtccctcaac
     1141 atggcggcca tggtcggtcc ggccacgccg gctgcgctcc acgcggtcag aatcgcgccg
     1201 taaatggccc cggtgttccg ggaaccgaac agctccgtca caaacgccgg acacgtggcg
     1261 aacccgccgc cgaagcaggt catgatgaca aaaaacgcag cctcgaagag cgcaacggag
     1321 gagacgctcg gaacaagggc aaagagtacg atctgaatac ccaccatcac cgcgaaagta
     1381 accggtcggc cgatgtaatc ggaaagcgcc gaccagccga ttcggcccgc ggcattgaac
     1441 acacccatga acccgaccat cacgctggcc tgcactgccg tcatcccgat gatttcctgc
     1501 gccatcgggg ctccgaagga aatcaaagac attcctgcgc tgatattgag gaagaacacc
     1561 acccacagaa gccagaaccg gggcgatttc aaggcttcgc caagcgtgtg cgtcttttcc
     1621 tgcagccccc cggccgtttc gctccgggga gtccatccgg cgggccggta atctgcgggt
     1681 gggaaccgca tcgcgcagga cgcgaggagg ataaccacaa aatacgccgc gccgagaatt
     1741 gcgaaggtga gaggcacctc gaccagggaa accagcttcg ttgcggcggg gcctgtcacg
     1801 agtgcggctg ccccgaaccc ggcgaccgcc attccgcccg ccagcccacg ccggtcgggg
     1861 aaccacttga ggagcgcatt aatcggtggt acgtacccca tcccgagccc gaatccaccc
     1921 agtattccgt aagttgcgta cagcacccac agcgagccca tgtgcgcggc aatcccggct
     1981 cctgcaatgc cggatccgaa gcagacagcc gacatcaccg cgcatcgtcg cggcccgcgg
     2041 cattccatcc atgctccacg gaatgccgag aaaaggccac cggtgagaaa cgcgagcgag
     2101 aacgccgtgg cgagttcagg acgcgaccag ccgtgcgcgg cctgcaatgg ctttatgaac
     2161 acactccacg cgtacaggga accgatacag aggtggatgg ccaccgcagc gatggcgacc
     2221 ttccatcgag tggccggggt gatcgtggaa tcgctcatgg taaggcatcc agcgggcatt
     2281 acgggcgcga aaacgggcga caaaagaata gcgcgggagc gcgggctgat caaagccg
//
LOCUS       DAOTNG010000408         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35861_length_2328_cov_3.692037, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000408 DAOTNG010000000
VERSION     DAOTNG010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35861_length_2328_cov_3.692037"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="P5179_13810"
     CDS             complement(<1..999)
                     /locus_tag="P5179_13810"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRS96340.1"
                     /translation="MAGECQIPLSVEMLREELRTLREQGAAEDVLDRIERDFAALPDG
                     APHGDLEVLYRRLEAVEPGPELAKREPTDLREIRGLRPEGPRRMRLECSDEALLDRML
                     GAWLARAAGCTLGKPVEGWTRQDIRALLEHVGEYPLRRYFPPVGQEWTGRRYHGWQLD
                     CLRGNIRYMARDDDMDYTLLGLVIMEAHGPGFTTRNVADEWLNTLPYHRVYTAETVAY
                     RNLVLGIQPPGTATLRNPYREWIGAQIRADFWGYAAAGLPELAAEFAWRDAILSHTRN
                     GVYGEMWMAATIAAAFAVNDVEQAIRIGLSEVPAECRLAKALNETLAWCNADGALET"
     gene            complement(1015..1986)
                     /locus_tag="P5179_13815"
     CDS             complement(1015..1986)
                     /locus_tag="P5179_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRS96341.1"
                     /translation="MPNTMRALQIVAPNDARVVTVDVPQIGAGDVLVRIKAATVCSHW
                     DLSMLGGWDIFERPGFPKYPISPGWPGHEVAGEVVAVGEGVDAFRVGDHVAAWSSRHS
                     QQEGKLGYYAEFAAIPAEDLLAVPESMPFEEAAILEMAMCVAASIRQAGDLSGRNVAI
                     GGAGAAGLLAVQMARSLGARRVDVFEPTESRRELALTLGAHAAFDPASDDAKALSPRT
                     YEVAFECAGAAASAQNLMRVTSGSVHLFGVVHGEIRFTMDHWSRNVHLFGYPGHSRES
                     AELGLALMACGGVKVSPIIGVTVGFDNYLDGIAGLKTGEAAKMCVVP"
     gene            complement(1997..>2328)
                     /locus_tag="P5179_13820"
     CDS             complement(1997..>2328)
                     /locus_tag="P5179_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96342.1"
                     /translation="VADSVGTRASLEQIIPLAHHESHLVLNGYYREGENLLDIQLLHG
                     REITTHAPAGWTRERLVATRDLVAKDTMHVRELMTHLMPVEKASEAYDLVLRKPTEFL
                     GICLKWQ"
BASE COUNT          398 a          801 c          697 g          432 t
ORIGIN      
        1 cgtttcgagg gcgccatcgg cgttgcacca cgcgagcgtc tcgttcagcg ccttcgcgag
       61 acggcattcg gccggtactt cgctcagacc gattctgatg gcctgctcca cgtcgttcac
      121 tgcaaatgcg gcggcgatgg tggcggccat ccacatctcg ccatagacgc cgttgcgggt
      181 gtggctgaga attgcatccc gccatgcgaa ttccgcggcg agttcgggta gtccagcggc
      241 ggcatagccc cagaaatcgg cacgaatctg cgcgccgatc cattcgcggt acggattgcg
      301 cagggtggca gtgccgggcg gttggatacc cagcaccaga ttgcggtacg ccacggtttc
      361 cgcggtgtag acacggtggt acggcaacgt gttcagccac tcgtcggcca cgttgcgcgt
      421 ggtgaagcct ggaccgtgag cctccatgat cacaaggccg agcagggtgt agtccatgtc
      481 gtcatcgcgc gccatgtacc ggatgttgcc gcgcaggcaa tcgagttgcc atccgtggta
      541 ccgcctgccc gtccactcct gcccgaccgg cgggaaatag cgccggaggg gatactcgcc
      601 gacatgctca aggagcgcgc ggatatcctg cctcgtccat ccttccaccg gcttcccgag
      661 agtgcaacca gcggctcgcg ccagccacgc gccaagcatt cgatccagaa gtgcttcgtc
      721 ggagcattcc agccgcatcc ggcgcggccc ctcagggcgc agaccgcgga tttcgcggag
      781 atcggtcggc tcgcgttttg caagctccgg gccgggttcc accgcctcaa gacgccggta
      841 gagcacctca agatcaccgt ggggtgcacc gtcgggcagt gcggcaaagt cccgctcaat
      901 ccggtccagc acgtcttcgg cagcgccctg ctcgcgcagt gtcctcagtt cctcgcgcag
      961 catttcgacg ctgagtggta tctggcattc ccctgccatt cctgtccctt cccgctacgg
     1021 cacgacgcac atcttggccg cttcgccggt tttgagtccg gcaattccgt cgaggtagtt
     1081 gtcgaacccc accgtgacgc cgatgatcgg gctcaccttc actcctccgc acgccatcaa
     1141 cgccagccca agttccgcgg attcacgcga atgccccggg tacccgaaca gatgcacgtt
     1201 gcggctccag tgatccattg tgaaacgtat ttccccgtgc accacgccga acaggtgcac
     1261 cgagccggat gtcacgcgca tgagattctg tgccgacgca gccgctcccg cgcactcgaa
     1321 cgcgacctcg tatgttcgcg gcgagagtgc cttcgcgtcg tcagaggccg ggtcgaacgc
     1381 tgcatgcgct cccagtgtaa gggcgagttc cctgcggctt tcggtgggct caaaaacgtc
     1441 cacgcggcgc gcgccgagcg accgcgccat ctggaccgcc agcaggcccg ccgcccctgc
     1501 gccgccgatt gcgacgttcc tgcccgagag gtcacccgcc tgccggatgg aagcggccac
     1561 gcacatggcc atctccagaa tggcggcttc ctcaaacggc atggattcgg gaacggcaag
     1621 caggtcctcc gcgggaatgg cggcgaactc cgcgtaatac ccgagcttcc cctcctgctg
     1681 cgaatggcgg gaactccacg ccgccacatg gtcacccacg cggaacgcat ccacgccttc
     1741 ccccacggca acgacttcgc ccgccacctc atggccgggc cagccgggcg atatggggta
     1801 tttcgggaac ccgggacgct caaaaatatc ccacccgccg agcatgctca ggtcccagtg
     1861 ggagcagact gttgccgcct tgatcctgac cagcacgtct ccagcgccga tctgcggcac
     1921 atccaccgtg acgacgcggg cgtcattcgg cgccactatc tgcaatgcac gcatggtgtt
     1981 cggcatgtct tcctcctcac tgccacttca ggcagattcc caggaattcc gttggcttgc
     2041 gcagaaccag atcgtatgcc tctgaagcct tttccaccgg catgaggtgc gtcatcagct
     2101 cgcgcacatg catcgtgtct tttgcaacca gatcgcgcgt tgcgacgagc cgttcccgcg
     2161 tccacccggc gggcgcgtga gtggtgatct cccggccgtg gaggagctgg atatccagca
     2221 ggttttcacc ctcgcggtag tagccgttca gcacaaggtg gctttcgtgg tgcgccagcg
     2281 ggatgatctg ttcgaggctg gcgcgtgtac ccacggaatc cgccacaa
//
LOCUS       DAOTNG010000409         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35878_length_2327_cov_4.524208, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000409 DAOTNG010000000
VERSION     DAOTNG010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35878_length_2327_cov_4.524208"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="P5179_13825"
     CDS             <1..190
                     /locus_tag="P5179_13825"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS96343.1"
                     /translation="HRLHAPVLTPWGFEATPAQILIFMRDHTNNHNGKLSVYVRMAGF
                     EPPFFVALGSDTFKQLVL"
     gene            388..>2327
                     /locus_tag="P5179_13830"
     CDS             388..>2327
                     /locus_tag="P5179_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96344.1"
                     /translation="MLIMCAGEFVVGGELKPGWERLVWNVPAAETLAVVLPEVVLVRD
                     SVEVETSDVTLITGEDFVVDYDHRRVVFLRPLPRGADVVIRYRRIPADWPVRQQWMRP
                     AQPDSADRDSIGAPRASVLARRQPLPESNLRIGGNKTFAVQVGSGRDLTLEQSLRVSI
                     NGTVAENVTVTAELSDQNLPIQPEGTTQELQEIDKVLVEIKSPHYRALLGSFDARNDI
                     GEFGRFSRRLDGAMGEASFDRWGASAGIASTRGEYRSLHLPIIDGNQGPYRLTDKNGN
                     TGIVVLAGTERVWVDGRLLVRGEPNDYTIDYSAGELTFTRKWLLRDDMEVVVDYEYSN
                     ESYARTTTLLSAYAQDGQRRGAWQTRVFFVQEGDDTGESFGVELDDTARALLAAAGDD
                     PAKASRPGWWYQPGGPYRLVDPSTEYFVKTTSDSGEYDVTFTRVGPGKGSYIRRHGYF
                     EQDMVYVGQGNGDWVPRITYPLPQRTRLTSLWTTYRPKSNFQMECEAAISDIDNNVLS
                     RVDDEDNTRFAGTISIETGLAPRDGQFGSINVRAKGRKIDEGFGGLYRTIDPYDNRRW
                     GLGVDESREQETTGEMTLTYRPGQNHTVDWDVGWLERNRLAERDGHTAWRTGLSWRTT
                     GDRMPSVRVSGEKIGMSIDQVMT"
BASE COUNT          479 a          679 c          708 g          461 t
ORIGIN      
        1 tcaccgcctc catgcacccg ttcttacgcc gtggggattc gaagccactc ccgcgcagat
       61 cctcattttc atgcgcgatc acaccaacaa ccacaacggc aagctctcag tgtacgttcg
      121 catggcagga tttgaaccgc catttttcgt cgcacttggc tcggatacgt tcaagcaact
      181 ggtcctttga catcgaacac cgcgcccttt ctgcatccgg ccggatggct gcgccgcggc
      241 tcgtcctttt ctcaaaccca tcctctcaca cgtaatttcc cgtctgcttc tgcgattctg
      301 cgctcgctgg caaagagccg gaagactcga tcgcccatct gccggtgggg gcctcctcgc
      361 gtccgtttac tggcgttttt cgcgaccatg ttgatcatgt gtgcgggcga attcgtcgtt
      421 ggaggcgagt tgaaacccgg ctgggaacgg cttgtgtgga acgttccggc ggctgaaacg
      481 ctcgcggtcg tacttcccga agtggtgctc gtccgcgatt cggtggaagt ggagacatcc
      541 gatgtcacgt tgatcaccgg tgaagacttt gtggtggatt acgatcatcg gcgcgtcgtt
      601 ttcttgagac cgttgccgcg cggcgccgac gtcgttatcc gctatcggag aattcccgcg
      661 gactggccgg tgcgacagca atggatgcga ccggcgcagc cagactcggc ggaccgagat
      721 tcgatcggcg caccccgcgc gtccgtgctt gcgcgccgcc aaccgttgcc cgagagcaac
      781 ctgcgaatcg gcggaaacaa gaccttcgcg gtgcaggttg gttcgggacg cgacctcact
      841 ctggaacagt cgcttcgcgt cagcatcaac ggtactgtcg ccgaaaatgt cactgttacg
      901 gcggaattga gcgaccagaa cctgccaatc caaccagagg gaacaacaca ggaactgcag
      961 gagattgaca aggttctggt ggaaataaaa agcccgcact atcgtgcgct cctcgggtca
     1021 ttcgacgcca ggaatgacat cggggaattc ggccgttttt cccgccggct ggatggggca
     1081 atgggtgagg cgtctttcga ccgatggggc gcctccgccg ggatcgcctc cacgcgcggc
     1141 gagtatcgca gtctccatct tcccataatt gacggcaatc agggacctta ccgactgacc
     1201 gacaaaaacg gcaacactgg tattgtggtt cttgcaggaa ccgaacgagt gtgggtcgat
     1261 ggcaggctgc ttgtccgggg cgaaccgaac gattacacga tagattactc ggccggtgag
     1321 ctcacgttta cgcgcaaatg gcttttgcgg gatgacatgg aggtggttgt agactacgag
     1381 tattcgaacg aatcctacgc acgaacgaca acgcttctgt ctgcatacgc gcaggacgga
     1441 caacgccgtg gagcgtggca gacgcgcgtg tttttcgttc aggaaggcga cgataccggc
     1501 gagtctttcg gtgtggaact ggatgacacg gccagagcac tactggctgc cgccggagac
     1561 gatcccgcga aggcatcacg gcccggctgg tggtaccagc ccggcggccc gtatcgcctt
     1621 gttgacccga gcaccgagta cttcgtgaaa accacttccg atagcggcga gtatgacgtg
     1681 acgttcacgc gcgtcggccc cgggaagggc tcttacatcc ggaggcatgg atacttcgaa
     1741 caggatatgg tttacgtggg ccaggggaat ggcgactggg tcccgcggat cacctaccct
     1801 ctcccgcagc gcacacggct tacatctctg tggacaacct accggccgaa gagcaacttc
     1861 cagatggaat gcgaagccgc gatcagcgat atcgacaaca acgtcctttc ccgtgtcgat
     1921 gacgaagaca acacgcgctt tgcgggaacg atctcgatcg aaacgggcct ggcgccccgt
     1981 gacggccaat ttggttcgat caacgttcgc gccaaaggca ggaaaatcga cgaggggttc
     2041 ggcggcctgt atcggactat cgacccctat gacaaccggc gctggggcct cggcgtggac
     2101 gagagcaggg aacaggaaac taccggagaa atgacgctta cgtaccgccc gggacagaat
     2161 cacacggtgg actgggacgt tggttggctg gaacgaaacc gccttgcgga acgcgacggg
     2221 cacacggcgt ggcgaaccgg tttatcgtgg cgcaccacgg gcgatcgcat gccttccgtg
     2281 cgggtttcag gtgagaagat cggcatgagc atcgaccagg ttatgac
//
LOCUS       DAOTNG010000410         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35949_length_2323_cov_5.790123, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000410 DAOTNG010000000
VERSION     DAOTNG010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35949_length_2323_cov_5.790123"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="P5179_13835"
     CDS             <1..1276
                     /locus_tag="P5179_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRS96345.1"
                     /translation="VFLLAIAVFAAAFYPRVRFDYDFSNLRSNIPASNLVKSKISKIQ
                     PMESAPAAIVVEDSAARMEVADALRAIQRADTLSPTIKEVRTLEGFVPADQDQKMALI
                     RRLRRVVEGHEEAILEGQSNINVEGLKRWLNVNEITTSDLPDFIVRKFRGPDGSLGEF
                     VVIGTSVSLRDGKQTIAFAEDIREIDTPHFGKFYASGSAVIFADMLLVMQKDSVIATT
                     VTLLVVFAIVLLQFGTKRAALLAILPVLVDGVLVAFSKITGAGFHAKFEELAWVFGAE
                     AVLGALLVFLFERKAFKTTILVMAPLLGGVILMIGIMALTGVRFNFYNIVALPSILGM
                     GVDNGVHFYARYREEGRGSVLYVLRTTGAAALMASLTTMEGFGGMLTADHRGLYSLGL
                     ISIIGMGTCLYMSVVFLPAWFAYREERHRKTD"
     gene            1307..>2323
                     /locus_tag="P5179_13840"
     CDS             1307..>2323
                     /locus_tag="P5179_13840"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRS96346.1"
                     /translation="MLVPSADIRHLHLIGICGTAMGALAVLLKERGFRVTGSDNAVYP
                     PMSEVLRDHDIPVMSAYSRDNITPPPDIVVIGNALSRGNEEVEAVLERKIPFVSLPEA
                     LREFFIRGSTSIVVTGTHGKTTTSSLLAWILDFAGLQPGFMIGGVPANFGVGARAGSG
                     RFFVSEGDEYDTAFFDKRSKFLHYLPDIVIVNNIEFDHADIFADIGEIELSFRRLLNI
                     IPRNGLVVANRDDSRVVRLTEHTWPPKVTFSLSGDANWQARNVCGTACGTAFDLVGPF
                     GQVTCDLPLFGRHNVSNALAVAAVCAHAGMSLEQIRDGMRSFKGVRRRLEKLYDAHEI
                     VLYDD"
BASE COUNT          455 a          607 c          714 g          547 t
ORIGIN      
        1 ggtgttcctt ctggcgattg cggtgtttgc cgcggcgttc tacccgcgag tgcgatttga
       61 ctacgatttc tccaacctgc gttcgaacat ccctgcgtcg aatctcgtga aatcgaagat
      121 ttccaagata caaccgatgg aatccgctcc tgcagcgatt gttgtcgaag actcggccgc
      181 gcggatggaa gtggcagacg ctctgcgtgc aatccagcga gccgacacac tgagtccgac
      241 gatcaaggaa gtgcgtaccc tggaagggtt cgttccggcg gaccaggacc agaagatggc
      301 gttgatcagg cggttgcgcc gcgttgttga aggacatgag gaagccattc tcgaagggca
      361 atccaacatc aatgttgagg ggctcaaacg ctggttgaac gtgaatgaaa tcacgacgtc
      421 ggaccttccg gacttcattg tccggaaatt ccgcgggccc gatgggagcc tcggcgaatt
      481 cgttgtcata ggtacttcgg tttcgcttcg cgacggcaag cagacgattg ccttcgcgga
      541 agatatccgg gaaatcgaca cgcctcattt cgggaagttc tacgcatcgg gcagcgcggt
      601 gattttcgca gacatgttgc tggtgatgca gaaggacagc gtgatcgcga ccaccgtgac
      661 gctgctggtg gtgttcgcca tcgtgctgct gcaatttgga acgaaacgcg ccgcattgct
      721 tgcgatcctg ccggttctgg tggacggtgt gctggttgcc ttctcgaaaa tcacgggagc
      781 cggcttccac gcgaagtttg aggaactggc gtgggtgttt ggcgcggaag cagttctggg
      841 tgctctcctc gtgtttctgt tcgagcgcaa ggcgttcaaa accacgatct tggtcatggc
      901 gccgctcctc ggtggtgtca ttctaatgat cggaatcatg gcgctgaccg gtgtgcgttt
      961 caatttctac aacattgtag ctctgcccag cattctcggc atgggcgtgg acaacggcgt
     1021 ccatttttat gcccgttatc gagaagaagg gcgtgggtcc gtgttgtacg tcctcaggac
     1081 taccggggcg gcggcgctga tggcttcgct tacgactatg gaaggtttcg gcgggatgtt
     1141 gacggcagac catcggggcc tctactccct cggtctcatc tcgataatcg gcatgggaac
     1201 gtgtttgtat atgtcagttg tcttcctgcc cgcttggttc gcgtaccgtg aggagcgaca
     1261 caggaagacg gactgagcgg acctcgtggc atcgcgggaa aaaggcatgc tggttccttc
     1321 tgcagatatc cggcatctgc atctcatcgg catttgtggt acggcaatgg gtgccttggc
     1381 cgtgctcctg aaagagcgcg ggttccgggt aaccggttcc gacaacgcgg tctatcctcc
     1441 gatgagcgag gtgctgcgtg accatgacat ccccgtgatg tcggcgtact cgcgggacaa
     1501 catcacgcct ccaccggaca ttgttgtcat cgggaatgcc ctgagccgcg gaaatgagga
     1561 agtcgaagcg gttctcgaac gtaagatacc ctttgtatcg ctgccggagg cgttgcgcga
     1621 attcttcatt cgtgggtcaa cctccatcgt cgttaccggc acacacggca aaacaaccac
     1681 aagttccctt cttgcgtgga ttctggattt cgcagggttg caaccgggct tcatgatagg
     1741 gggtgtgcca gcgaactttg gcgtgggcgc gcgcgctgga tccgggcgct tctttgtcag
     1801 tgaaggcgac gagtacgata ccgcgttttt cgacaaacga tcgaaattcc tgcattacct
     1861 tcctgatatc gtcatcgtca ataacatcga attcgaccac gcggatattt tcgcggacat
     1921 cggcgagata gaactgagct tccggagact tctcaacatc attccccgaa atggcctcgt
     1981 ggtggcaaac agggatgact cacgggttgt ccggcttacg gaacacacgt ggccgccgaa
     2041 ggtgacgttt tcgctttcag gagacgccaa ctggcaggcg cgcaacgtgt gcggtaccgc
     2101 gtgcggcact gcgtttgatc tcgttggacc attcgggcag gtaacctgtg atttgcctct
     2161 gtttgggcgc cacaatgtca gcaacgcgct ggcggttgcg gcggtgtgtg cgcatgctgg
     2221 catgtcgctg gaacagatac gagatggcat gcggagcttc aagggtgtaa ggcggaggct
     2281 cgagaagctt tacgacgcgc acgagattgt tctttacgac gat
//
LOCUS       DAOTNG010000411         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_35953_length_2323_cov_3.896384, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000411 DAOTNG010000000
VERSION     DAOTNG010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35953_length_2323_cov_3.896384"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="P5179_13845"
     CDS             <1..599
                     /locus_tag="P5179_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96347.1"
                     /translation="SHTYALRAGTFAGRGNVRSGAEPVGRGTLGYMTNATIRGLADGV
                     YYWSVQAVDNGLARSEWAPEQSFVIDTKPPRVTRLRGDTLSVGLDRVINVAIDFDDAL
                     TGVDTLSAIDVTLSLVGQPPFRSPSSGGTRATSGSGKHQFRPAVSCLSRSGCASGTYV
                     IFAVTSSPIPPSLPGSSSRQHSASRGRRAVRSRTRQEP"
     gene            707..1279
                     /locus_tag="P5179_13850"
     CDS             707..1279
                     /locus_tag="P5179_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96348.1"
                     /translation="MEFTPDVVVGTLAVPAVLTMKYGASPRVARGAVVASNLHIFRLD
                     GSTWTYIGGLADDATKTISVPVNHLGTYALFEASGNVTDATLGDLTCTPRVFSPGTTQ
                     GYSDKTVIAFTVSTADAGNSATISIFNTAGRLVREMAPSIVAGANSIAWNGTDDGGSI
                     APSGMYIVAVKVGGVEKRQTVVILNKYARP"
     gene            1345..>2323
                     /locus_tag="P5179_13855"
     CDS             1345..>2323
                     /locus_tag="P5179_13855"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS96349.1"
                     /translation="MRMHSAHTRRLRQGTTGVACSIGIMCMVAAAHAGTPSTTVGVGA
                     RPLGMGGAFVGLADDAHAIYWNPAGLPRLQRQEFTGSYMPNRLISGLNTGYGAATIPI
                     GERQAVGADMFYESYSDNELSFTSFNLRASYGLSLFNWLSVGGNAKYVAPLSVKYNNN
                     QVDDPRGFGFDVGTLLDFGNLLPALEGLRVGAVVRDVGGTSVKHDNDFSENIYPATYV
                     VGASYKLNDSFTFAADLDDRARFGVEYSLLNLLSLRAGIQRTVQGYGTGMYYNGGAGI
                     KWRGIKLDYAYETHPTLNPTHYMTLSFVYNPSFVTIRDARINRTPLFRAL"
BASE COUNT          430 a          710 c          748 g          435 t
ORIGIN      
        1 cgtcccacac gtacgccctg cgcgcaggca cgttcgccgg acggggcaac gttcgttccg
       61 gggcggagcc ggtgggaagg ggtacgctcg ggtatatgac caacgcgacc atccgcggac
      121 tggcggacgg ggtgtactac tggagcgtgc aggcggtgga taacggactt gcgcgctcgg
      181 aatgggctcc cgagcaatcc tttgtaatcg acacgaagcc tccgagagtt acccgcctgc
      241 gcggcgacac cctcagcgtc ggcctcgatc gcgtgatcaa cgtcgcgatt gattttgatg
      301 acgcgctgac cggggttgac accctttccg cgattgacgt cacgctctcg ctggtcggac
      361 agcctccgtt caggtcacca agctccggtg ggacccgggc gacgtctggt tcgggcaagc
      421 atcaattccg accggcagtt tcctgtttga gccggtcagg gtgcgcgtcc ggaacgtacg
      481 tgatattcgc ggtaacgtcc tcgccgatac caccttcgct acccggctcg tcttcacggc
      541 agcacagcgc atcgcgcggg aggagggcgg tacgatcacg aactcggcag gaaccataag
      601 cctttatgtt ccaccgcgga gtttcgaccg cgaggtaggc atttcgcttg cgcaaccgga
      661 ttccgcgagc cttcccgccg ggccggggcc gggactcggt ctggcggtgg agtttacgcc
      721 tgacgttgtg gtgggcacgc ttgcggttcc ggcggtactg acaatgaaat acggtgcttc
      781 gccccgggtg gcgcgcgggg cggtggtggc gtcgaatctg cacatcttcc gcctggatgg
      841 ttccacgtgg acgtatatcg gcgggcttgc ggatgacgcg acgaaaacta tctcggtgcc
      901 ggtcaaccac ctcggaacgt acgccctgtt tgaggcatcc gggaacgtaa cggacgcgac
      961 actcggcgac cttacctgca cgccgcgcgt attttctccc ggcacgacgc aagggtacag
     1021 cgacaagaca gtgattgcgt ttaccgtgtc aaccgcggac gcgggcaatt ccgcgacgat
     1081 cagcatcttc aacaccgcgg ggcggctggt gcgggagatg gcgccctcca ttgttgccgg
     1141 cgccaattcc atcgcgtgga acggcacgga tgacggcggc agcatcgcac ccagcgggat
     1201 gtacatcgtc gccgtgaagg tcggaggagt cgaaaagcgt cagacagtgg tgattctgaa
     1261 caagtacgcg cgtccatgac cacgatacat tgagcccgcg tcaaaccggt tcaggggttg
     1321 tcatacgaca taaggtgagg cgttatgcgg atgcactcag cgcacacacg aaggcttcgt
     1381 caggggacga ctggcgttgc ctgttcaatc ggcatcatgt gcatggttgc ggcggcacat
     1441 gcggggaccc ccagcacaac ggtgggagtg ggggcccgac ctctgggaat gggtggtgcc
     1501 ttcgtcggcc tcgcggatga cgcgcacgcc atctactgga atcccgccgg tctaccgcgg
     1561 cttcagcggc aggaattcac cggcagctac atgcccaacc gcctcatcag tggcctcaac
     1621 accggttacg gggccgccac gatccccatc ggggaacggc aggcggtcgg cgcggacatg
     1681 ttctacgaat cgtacagcga caacgagctg tcgttcacat ccttcaatct gcgcgcgtcg
     1741 tacggtttgt cgctgttcaa ctggctgtcg gtgggcggga acgcgaaata cgtcgcgccc
     1801 ctttccgtga aatacaacaa caaccaggtg gacgatccgc gcggattcgg cttcgatgtc
     1861 ggcacgctgc tggatttcgg gaacctcctc ccggcgctgg aagggctgcg ggtgggagca
     1921 gtcgtgcggg acgtcggggg aaccagcgtc aagcacgaca acgacttctc ggagaacatc
     1981 taccccgcca cgtacgtggt cggagcgtcg tacaaactga acgacagctt cacctttgcc
     2041 gcggatctgg atgaccgcgc gcggtttggc gtcgaataca gcctgctcaa cctcctttcc
     2101 ctccgggcgg gcattcagcg gacagtccaa gggtatggca ccggcatgta ctacaacggt
     2161 ggcgcgggta tcaagtggcg ggggatcaag ctcgattacg cgtatgagac ccacccgacg
     2221 ctgaacccga cgcactacat gacgctgtca ttcgtctaca acccgtcgtt cgtgacgatc
     2281 cgggatgccc ggatcaaccg gacgcctctc ttccgggcgc tgt
//
LOCUS       DAOTNG010000412         2316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_36093_length_2316_cov_5.318443, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000412 DAOTNG010000000
VERSION     DAOTNG010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36093_length_2316_cov_5.318443"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5179_13860"
     CDS             complement(<1..323)
                     /locus_tag="P5179_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96350.1"
                     /translation="MSSWFPRGEFPVPDKSVQKALARAEEAINGIAKGTEGVPCAWGR
                     VHNGGNAGFSVHAMRRTATRLPWMVVHFAFHEHPAGVVCSYRTRYSVMSLLLLLGVSV
                     VFLAFP"
     gene            561..731
                     /locus_tag="P5179_13865"
     CDS             561..731
                     /locus_tag="P5179_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96351.1"
                     /translation="MFFEHMHRRAFVMVVSDVVVRELAGAPPRVRALGEKMLPLAEVV
                     PVTQEALALRDA"
     gene            932..1111
                     /locus_tag="P5179_13870"
     CDS             932..1111
                     /locus_tag="P5179_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96352.1"
                     /translation="MKTKRKRFDCVAMKRRAAERIYRETAGLTREQEIEYWRRSIDVL
                     KTRKVVTPETPRATC"
     gene            1141..1647
                     /locus_tag="P5179_13875"
     CDS             1141..1647
                     /locus_tag="P5179_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HRS96353.1"
                     /translation="MQVQKKLFHCGDVLLATMDKEIEEVLAAVHAVEWRDSFEYTHGG
                     TVYRHQAAYNLALAAEFGTMGWDPQPVLRTDPKLIGDFRKGLVFVEVQFGNAAALYRD
                     FYKFRYGLANGLLSLAVLVVPSDPVRFFPTRRGSVRNMADFGLADRCLSVLPVNVPTL
                     LLGLLPAA"
     gene            1777..>2316
                     /locus_tag="P5179_13880"
     CDS             1777..>2316
                     /locus_tag="P5179_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRS96354.1"
                     /translation="MNAVLIERAHELGFREVPEAPMGDYHARTRLVAGSICNSTDAKL
                     LHGTFPGCTAFPAVLGHEAVGEVTAVGAKVRNYRVGDLVIRPRVCFPPEFNIREYFGS
                     FADFAYVTDQWAMDEDNPPTGARAFGHPQQVLPAGTDPMLGVLSITLKETLSWIRRFA
                     VGKGHRVLVFGTGPVGTAFV"
BASE COUNT          485 a          663 c          696 g          472 t
ORIGIN      
        1 gggaaggcga ggaacaccac agagacaccg agcagaagaa gcagcgacat cacggagtat
       61 cgtgtccgat acgagcagac cactcccgcc ggatgctcgt ggaacgcgaa atgaacaacc
      121 atccacggca ggcgggtggc ggtcctcctc atggcgtgca cactgaaacc tgcattgccg
      181 ccgttgtgaa cgcgacccca tgcacacgga acaccctccg tgcccttcgc aatcccattg
      241 attgcttctt ctgcgcgtgc aagggctttc tgaaccgatt tatcgggtac ggggaactct
      301 ccccggggaa accatgagga catgaggcgg cccctttgcc attcaagtgc cgttccacca
      361 caggttttgc cgcgacagat acgacacgaa cacacacatc accatgaatg tacgaatcca
      421 caacggaatt cgtattttca ctttagcctc acaatgttgc tgtcggggga atccgatgcg
      481 aacgacactg ttgcgcgtgt acgtcgaaac ctccgttttc gggggccgtg ttcgatgacg
      541 agtttcgaga acggagcagc gtgtttttcg aacacatgca caggcgggcg tttgtgatgg
      601 ttgtgtctga cgttgtcgtg cgcgaactgg ccggtgcacc gcccagagtg cgagcgctgg
      661 gggagaaaat gcttcctctg gcagaagtgg tccccgtcac gcaggaagca ttggccttgc
      721 gggacgcata actgtcggcg ggtgttctcc cgcctcccgc atccaacgac gctcttcata
      781 tcgcactggc cacagtgagc ggttgtggga tgatcgcgag ctggaatttc aaactattct
      841 gaactacagg agaattcccc ggttcaatgc agtgaacgct ttgaacggtt cccggggatc
      901 atgattcatt ctcctctgga gatcgttggc aatgaagacg aaacggaaac gctttgactg
      961 cgttgcaatg aaacgcaggg ccgcggagcg catctatcgg gagacagccg ggctgacacg
     1021 ggaacaggag attgagtact ggcggaggag tatcgacgtg ctcaaaaccc gaaaagtggt
     1081 caccccggaa actccccgag ccacctgcta aactccttgc ttctgcggaa tcgtggaccg
     1141 atgcaggttc agaaaaagct atttcactgc ggagacgtcc tccttgcaac catggacaag
     1201 gagattgagg aggtgctcgc cgcggtacac gcagtggagt ggagggattc cttcgaatac
     1261 acacacggag gcacagtgta ccgacaccag gcagcgtaca atctggcgct ggcagcggaa
     1321 ttcggaacga tgggctggga tccgcagcct gtgctccgta cggacccgaa actcatcggt
     1381 gatttccgca aagggctggt gttcgtcgag gttcaattcg gtaacgcggc ggcactttac
     1441 cgggattttt acaagttccg atacgggttg gcaaacggtc tgttgtccct tgccgtgctg
     1501 gttgtaccgt ctgacccagt ccggtttttc ccgaccagac ggggaagcgt tcggaacatg
     1561 gcggactttg gtcttgcgga ccgttgtctt tccgtgttgc ccgtgaacgt gccaacattg
     1621 ctactcgggc tcctgccggc agcgtaacag aacccttccc ggagccagat tccccgctct
     1681 tctctgaccc tccttgctcc ctgacagggt accattacac cctatggtac cggacgctcc
     1741 aaccggaggg gttaccgcgg aaaaggagtt acgcagatga acgcagtcct gatcgaacgg
     1801 gcgcacgaac tggggtttcg tgaggtgccc gaggctccca tgggagacta ccacgcccgg
     1861 acgcggctcg ttgcgggcag tatctgcaac agcacggacg cgaagctcct gcacggcacc
     1921 tttcccggat gcaccgcatt tccggcggtg ctgggccatg aagcggtcgg cgaagtgacg
     1981 gcagttggcg cgaaggtgcg caactatcgc gtcggagatc tggtcattcg gccgcgggta
     2041 tgcttcccgc ccgagttcaa catcagggag tatttcggct cgtttgccga tttcgcgtac
     2101 gtgaccgacc agtgggcaat ggacgaggac aatccgccca ccggtgctcg tgcgttcggc
     2161 cacccgcagc aggtgcttcc ggcgggcacc gaccccatgc tcggggttct ctccatcaca
     2221 ctgaaggaga cactgagctg gattcgacgg ttcgccgtgg gaaaaggcca ccgcgtgctc
     2281 gtgttcggaa caggaccggt gggaaccgct ttcgtg
//
LOCUS       DAOTNG010000413         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_36214_length_2311_cov_3.875887, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000413 DAOTNG010000000
VERSION     DAOTNG010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36214_length_2311_cov_3.875887"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="P5179_13885"
     CDS             complement(<1..586)
                     /locus_tag="P5179_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRS96355.1"
                     /translation="MKGKPSHTASLDPAANPSYHDVVSCRFTPQQKDPAVGPKFRLAV
                     TSLLVTRIEESIPEAFREAANRGADLILLPEEPDIVMGWKPGLYRLTDHPIYSRMASF
                     AAEYGIGAVASVSAVAGNGFANTGFLIDRKGALAGVYRKQHPAPGEEAIVTDQAGDDP
                     FPVFDFDGVVLGIAICMDVHFPEMFCIYGLKCADI"
     gene            658..>2311
                     /locus_tag="P5179_13890"
     CDS             658..>2311
                     /locus_tag="P5179_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96356.1"
                     /translation="MIADIATDLVSLQASFSSASDEIKSLIVRISSEIDFVNKLSELH
                     AEEAEAWRIFAREALDVVQGSVAGGKMDSIRGAVAEAEAILAPVAYTAKSYTIWCVGH
                     GHIDMNWMWSWPETVAVTNDTFSTVDRLMSEYPDFCYSQSQTSVYEIARKYNPTLFQR
                     IKERVAEGRWEPTACMWVEGDKNLAGGESLCRHLLYTRQFMEEHFGLKPADVLIDWEP
                     DTFGHAHTIPHFLARAGVRRYYFCRGGADPKPSVFWWEGPDGSRVLCYREITWYNWEV
                     RPELTNHLVAFAKETGLRAWMNVYGVGDHGGGPTREHIEWAHRMDSWPVFPSIKLSTT
                     KPFYELLEENSAKWDVLKRELNFEFTGCYTTQTSIKRNNRFAEQLLYQAEAASSLAWL
                     EAAQEYPEPTLRDGWINTLFSQFHDILPGSCVRPSREYNSGQYQETAAGAGMALTNSL
                     RAVASEIDTACFGGSGGVGASANTIGGGAGFESGMGAVSAYDGGSSDVRPFVLCNLNG
                     WERSEVLIATVWNSGWKDIVVQDDAGNRFLGQIVEKGGYWGHE"
BASE COUNT          503 a          626 c          721 g          461 t
ORIGIN      
        1 caatgtcagc gcatttcaac ccgtaaatgc aaaacatctc cgggaagtga acgtccatgc
       61 agattgcgat cccgagaacc acgccatcaa agtcaaacac cgggaaagga tcgtctcccg
      121 cttgatcagt cacaattgct tcttcgccgg gggcaggatg ctgcttccgg tacacgccgg
      181 caagagcacc ttttctgtca atcaggaatc cggtattcgc aaagccgttc cctgccacgg
      241 ctgagaccga tgccacggca ccgatcccgt actccgcggc aaaggaggcc atccgggagt
      301 agatcgggtg gtcggtgagc cggtaaaggc cgggtttcca acccattacg atatctggct
      361 cctccgggag caagatgaga tctgcgcccc ggttggccgc ttcgcggaag gcctctggaa
      421 tagactcctc gattcgggtg acgagtaacg aggttacggc aagccggaat ttcggtccca
      481 cggcggggtc tttctgttgc ggggtgaaac ggcacgaaac gacgtcatgg taggacgggt
      541 tggcggccgg gtcaagggat gcggtatgag agggttttcc cttcatgccg ctgttaggta
      601 ccatttgaga tactcaaacg gccacccttc acgccaacct ttgcgaagga tctttcaatg
      661 atcgcagaca tcgccaccga tctggtttcg cttcaggctt cgttttcatc cgcttcagac
      721 gagattaagt cgctgatagt ccgcatatcg tcggaaatcg attttgtgaa caaactgagt
      781 gaactccatg cagaagaagc cgaggcatgg agaatcttcg cgcgagaagc tcttgatgtc
      841 gttcagggaa gcgttgcggg tggaaagatg gactccatcc gcggtgcggt ggccgaagcg
      901 gaggcgatcc tggcgccggt ggcgtacacc gcgaaaagct acaccatctg gtgcgttggt
      961 cacggtcaca ttgacatgaa ctggatgtgg tcctggccgg aaaccgtggc agtgacgaac
     1021 gacacattca gcacggtgga ccggttgatg tcggagtacc ccgacttctg ttattcgcag
     1081 agccagacaa gtgtatacga gattgcccgc aagtacaatc caacgctgtt tcagcggatc
     1141 aaggagcgtg tagccgaggg caggtgggaa ccgactgcct gcatgtgggt ggaaggcgat
     1201 aagaacctcg cgggagggga gagcctctgc cgtcatctcc tgtatacccg tcagttcatg
     1261 gaagagcact tcggcctcaa gcccgcggac gtcctcattg actgggaacc cgatacgttc
     1321 ggtcacgcac acacaatccc gcatttcctc gcccgggcag gcgttcggag gtattacttc
     1381 tgccggggag gagccgaccc gaagccgtcc gtgttctggt gggaggggcc cgatggttcc
     1441 cgggttcttt gctaccgcga aatcacgtgg tacaactggg aagtcagacc ggaactgacg
     1501 aaccatctgg tcgcgttcgc gaaggagacc gggcttcggg cctggatgaa tgtgtacggg
     1561 gtgggggacc acggcggggg ccccacgcgc gaacacatcg agtgggcaca ccggatggat
     1621 tcgtggccag tgtttccgtc catcaagctt tcgacgacga agccctttta cgaactgctg
     1681 gaagagaatt ccgcgaaatg ggacgtgctc aaacgagaac tgaacttcga gttcaccggc
     1741 tgctacacca cccagacgag catcaaacgt aacaaccggt ttgcggaaca actgctctac
     1801 caggcggagg ccgcgtcctc gctcgcgtgg ctggaagccg cacaagagta tccagaaccg
     1861 actttgcggg atggctggat caacacgctt ttctcccagt ttcacgacat tcttccgggt
     1921 tcgtgcgtgc ggccttcccg ggagtacaac tcaggtcagt accaggaaac tgccgcaggc
     1981 gccgggatgg cgctgacgaa cagccttcgc gcagtcgctt cggagattga tacggcatgc
     2041 ttcggcggga gcggaggggt cggcgcgtca gcaaacacaa tcggtggtgg cgcggggttc
     2101 gaatcaggga tgggtgccgt ctcggcatac gacggcggct caagcgacgt aaggccgttc
     2161 gtgctctgca acctcaacgg gtgggaacgc agcgaggtat tgatcgccac tgtgtggaat
     2221 tccggttgga aggatattgt ggttcaggac gacgcgggaa accgttttct cggacagatc
     2281 gtagaaaaag gcgggtactg gggccacgaa t
//
LOCUS       DAOTNG010000414         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_36945_length_2277_cov_3.981548, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000414 DAOTNG010000000
VERSION     DAOTNG010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36945_length_2277_cov_3.981548"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>2277
                     /locus_tag="P5179_13895"
     CDS             196..>2277
                     /locus_tag="P5179_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRS96357.1"
                     /translation="MRFYVRYEGIYRITGADLKKWTQGSPIIWETVDPRTFRLEESGR
                     EIPIYVEGEERGVVEDSLAIEFFARPNIGRYQSVAPDLYRDPFVEMGIYNLSWGHGPG
                     ARLSDEDGSLTTPSSSADLVQCLTYWHTVHAEEDQTFERFGGANEDLIDRYMWKRIGH
                     GDTAEFPIQLESPVNDAKNPPIVEVMARGATFSRHIMEVRLQGTRIGTAGLSPILRDL
                     DLIHFKRNSTPVTLEDGVNTLSVITGDPSNPDPNGNDVVALNWFEVTYERNYVAKGNS
                     ISFRRPSEPSQGVFEFSLSGFGTPDITVYKVGVSRIRNFTIETPITSRSEGYTIRFQD
                     RIDGDDPRYIAVASSRKLKPDRVAVVPVWERPLTDRTRAADYLIVTHSVLLDSVARFA
                     EYRRSAQGGSHTVAVVDVAQIYDEFGYGYPAAEPVRDFLNYAFRYWAQPSLRYVVLVG
                     DGVMRYPSDNMGLLAYPLIPAFLEVQARWGGTETDHPYAMLAGDDLLPDIIVGRIPAS
                     TTNDLRVAVDKIIEFEQTPERSKWRNSVLLISEGEAWFVAQHEFLGAELPPSYFQKKL
                     YNGATTAPHLDVFYGRRGESFALLNEGSLWTIYLGHGGGGVWGSDRLLVHADPPTLQN
                     AGRAGIFLSMTCFTGAFAGVTQKSLAELMLFSRGGAIAWLGASSVGWVNNDFYFTQSI
                     IRAAFAQSRERQ"
BASE COUNT          472 a          614 c          691 g          500 t
ORIGIN      
        1 gttcgctgga cgaagtcgct tcgcgtcgcg gtgcgctttg cctcggagag cacggtgccg
       61 ctagcgagag ccgcgaggca agataccgat catcctgcgg tgggcgccct caatgccgat
      121 ttgataccgc tattcggacg aacggggacc gctcaacccg cggaacgggt tgcggcgtct
      181 cagaactcat atcgcgtgcg tttttatgtc cggtatgagg ggatttaccg catcacaggc
      241 gccgatctga aaaaatggac acagggcagt cccataattt gggaaactgt cgacccccgc
      301 acgttccggt tggaggaatc gggcagggag atacccatat acgtggaagg tgaggaacgt
      361 ggcgttgtcg aagactcgct cgcgattgag ttcttcgcgc ggccgaacat cggcaggtac
      421 cagtccgttg cgccagacct gtaccgggac cccttcgtgg aaatggggat ttacaacctt
      481 tcgtggggcc acgggccggg cgcacgcctt tctgatgagg atggttctct gacgacgccg
      541 tcttcttcgg cagatctggt tcagtgcctt acctactggc ataccgttca tgcggaagaa
      601 gaccagacgt ttgagcgatt cggcggcgcg aatgaggatt tgatagatcg ttacatgtgg
      661 aaaaggattg gccacgggga tacggcagaa ttccccatcc agcttgaaag ccctgtgaat
      721 gacgcgaaaa atcctccgat tgttgaggtc atggctcggg gcgcgacgtt cagccggcat
      781 atcatggagg tccgattgca gggaacccgg atcgggacgg caggtctttc gccgatcctg
      841 agagatctgg atctcatcca tttcaagcgc aactcaacac cggtcacact ggaggacgga
      901 gtcaacacgc tctcggtcat caccggagat ccctccaatc cggatcctaa cgggaatgat
      961 gtcgtcgcat tgaactggtt cgaggttacc tacgaacgga actatgtggc gaagggcaat
     1021 tcgatttcct tccgtcgccc ttcggagccc tcacaggggg tgtttgagtt ctctctgtcc
     1081 ggcttcggca cccccgatat taccgtgtac aaagtaggcg tgagccgaat acggaacttc
     1141 accattgaaa cccccatcac atcccgttct gagggatata cgattcgctt tcaggatcgt
     1201 attgacggcg atgatccccg gtacattgcg gtggcctctt cccggaagct caaacccgat
     1261 cgcgtggcgg tggtgccggt gtgggaacgt ccgctgacgg acagaacacg cgccgctgat
     1321 taccttatcg ttacccattc cgtgctcctg gattccgtcg cccggttcgc cgaataccgc
     1381 aggagcgcgc aggggggaag ccacactgtg gcggttgtgg atgtggcgca gatatacgac
     1441 gaattcggat atggataccc cgccgcggag ccggttcgag atttcttgaa ttacgcgttc
     1501 cggtactggg cgcaaccctc gttgcgttac gttgtgttgg tgggtgacgg ggtcatgaga
     1561 tacccgtcgg acaatatggg gctgctggcg tatccattga ttcccgcgtt tctggaagtg
     1621 caggcaagat ggggaggtac tgaaaccgac cacccctacg cgatgctggc gggcgatgac
     1681 cttctccccg acataatcgt ggggagaata cctgcttcca cgacaaacga tctccgtgtc
     1741 gccgtggaca agatcatcga atttgagcag actccggaac gatcaaaatg gcgaaactcg
     1801 gtgttgctga tttccgaagg cgaagcatgg ttcgttgcac agcatgaatt cctcggtgca
     1861 gaacttccac caagctactt tcagaagaaa ctgtacaacg gggccacaac ggcgccccat
     1921 ctcgatgtgt tctatggaag acgtggggaa agctttgcac tactcaacga gggttcgttg
     1981 tggacgatct atctgggaca cgggggaggt ggcgtgtggg ggagcgaccg cctgcttgtt
     2041 cacgcggacc cgcccaccct gcagaacgcc ggcagggcag ggatattttt gagcatgacg
     2101 tgtttcacag gggcttttgc cggagttacc cagaagtcac tggccgaatt gatgcttttc
     2161 tcccgcggtg gcgcaatcgc ttggctgggt gcgtcgagcg tcggctgggt gaacaatgat
     2221 ttctacttca cccagtcgat cattcgtgcg gcattcgcgc agtcccggga acgacag
//
LOCUS       DAOTNG010000415         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37110_length_2269_cov_5.299458, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000415 DAOTNG010000000
VERSION     DAOTNG010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37110_length_2269_cov_5.299458"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="P5179_13900"
     CDS             <1..577
                     /locus_tag="P5179_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HRS96358.1"
                     /translation="ARAFIGHMRVRAADGSVRDHREVFIVDIPEDITRPGPAGPLEGT
                     GTTFPAPPAGTSQRRLTHTDSGCFGLVRSTSDGGMIAFLSRAADGTPQVFVVSPLGGE
                     PRQITSFPGGASHPVRWLPDDLHFVTVSEGRVYVVDAASGCARAITEPSRSRPDGLVV
                     SPDGKLVAFNREVVYASGKFAQVFVADIELP"
     gene            643..1923
                     /locus_tag="P5179_13905"
     CDS             643..1923
                     /locus_tag="P5179_13905"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRS96359.1"
                     /translation="MTEAAESREAQRRPNGTEVRHMWDCGHPNPALRGAGFPVLDDVR
                     HSLIFAGERQAGAYNHHSKLTRHRGMFHAMWSNHPHGEDGPGQRVLYSHSSDGVRWSS
                     SVELFPPMQPPRPNGENGLVLTAWRWVKYEGRLFATAMCNENIGFENPDGSEIVPQRD
                     AGHRFLARRFFDGFCREIGENGPICPPILPMGDKVPPPAPAFVSLYSDDEEAMRLGRR
                     VREEMCRPENTPSWNPPGISLPRGVDTLRLCEPTVYRARCGEYVMLLRDGEFSHRLYA
                     SFSDDGRRWSSAVPTDIPDSPSLSTTVALPNGSVLLVGNMVASDFDNAAEVRHYLRDP
                     LVIAVSSDGSMFERAYALRCGTQQWRTPREEVRGRNGGAQYPSCVVENGILHVQYSMG
                     KEDIWMSSVALACLGPDGGGSLNERGTGVRSQGP"
     gene            1920..>2269
                     /locus_tag="P5179_13910"
     CDS             1920..>2269
                     /locus_tag="P5179_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent oxidoreductase"
                     /protein_id="HRS96360.1"
                     /translation="MNAEVLVEAEHFDDLGGWTLDTGFVESMGSPYLMAHGLGTPVAD
                     ATTTVRLPEPGAWRVWVRTFDWVARWDASGCPGRFEVLVDGEPVGVVFGTEGADWHWQ
                     DGGVIQIGKPDIRVS"
BASE COUNT          408 a          675 c          758 g          428 t
ORIGIN      
        1 ggcgcgggca tttatcgggc acatgcgggt gcgtgcggcc gacgggtctg ttcgggacca
       61 ccgcgaagtg ttcatcgtgg atattcccga ggacatcacc cggcccggtc ccgcagggcc
      121 gcttgaggga accggcacca cgtttcccgc cccgccggcg ggcacttccc agcggcggct
      181 gacccatacg gattcgggat gcttcggcct tgtgcgttcc acctcagacg gcgggatgat
      241 cgcattcctt tcccgcgcgg ctgacggaac gccgcaggtg tttgtcgttt cacctctggg
      301 aggagagccc cggcagataa cgagcttccc cggaggagcc agccaccccg tacggtggct
      361 tcccgacgac ctgcactttg ttacggtgag cgagggtcgt gtgtatgttg ttgacgcagc
      421 cagcgggtgt gcgcgggcga tcactgagcc ctcccgcagc cgcccggacg ggttggtcgt
      481 atcccctgac gggaagctcg tggcgttcaa ccgggaggtc gtctacgcaa gcggcaagtt
      541 cgcgcaggtg ttcgttgcgg atattgagtt gccgtagata gacgccttcc tcaaccagac
      601 ccgcgaaagg aagctggcgc atccgcagct ttggaacccg ccatgacgga agcagcagaa
      661 tcgagagaag cgcagcggag gccgaacgga acggaagttc ggcacatgtg ggattgcggc
      721 cacccgaacc ccgcgcttcg cggcgcaggg tttcccgtgc tcgatgatgt caggcattcc
      781 cttatcttcg ccggcgaacg gcaggcaggc gcgtataacc atcacagcaa gctcacgcgg
      841 caccggggca tgttccacgc gatgtggagc aaccacccac acggggaaga cggaccggga
      901 caacgagtgc tttactccca ctcgtcggac ggagtgcgct ggagcagttc cgtcgagttg
      961 ttcccaccga tgcagccccc gcggccaaac ggggaaaatg ggctcgttct gacggcgtgg
     1021 cgctgggtga agtacgaagg ccgcctgttt gccactgcga tgtgcaacga aaacatcggt
     1081 tttgagaacc cggacggcag cgagatagta ccgcagcgcg acgccgggca tcgattcctc
     1141 gcaaggaggt tcttcgacgg tttctgccgc gaaatcgggg aaaacggtcc gatttgcccg
     1201 cccatccttc cgatgggaga caaggtgcct ccgcccgctc cggcttttgt gtctctgtat
     1261 tcggacgacg aggaagcaat gcggctcgga cgtcgggtcc gtgaggaaat gtgccgccct
     1321 gagaacacgc cgtcatggaa ccctcccgga atcagcctgc cccgtggcgt agataccctc
     1381 cgactctgcg aacccaccgt gtaccgcgcc agatgcgggg aatacgtgat gttgctgcgc
     1441 gatggcgaat tctcgcaccg cctgtacgcc agcttctccg atgacgggcg caggtggagc
     1501 tcggccgttc cgacggatat tcccgattcg ccatcgctgt cgaccaccgt ggctttgcca
     1561 aacgggagtg ttctgctggt gggcaacatg gtggcgtccg attttgacaa tgcggccgag
     1621 gtccggcatt acctgcgtga tccgcttgtg attgccgtaa gcagtgacgg cagcatgttc
     1681 gagcgcgcgt atgccctccg gtgcggcacg cagcagtggc gcactccgcg cgaagaagta
     1741 cgtggccgaa acggtggcgc gcagtacccc tcctgtgtgg tcgaaaacgg gatcctccat
     1801 gtccagtact cgatgggcaa ggaagatatc tggatgagtt ctgttgcgct ggcctgcctc
     1861 ggtcctgatg gaggaggttc cctgaacgag cgaggaacag gtgtgaggag ccaagggcca
     1921 tgaacgccga agtgctggtc gaagcagaac atttcgatga ccttggtggg tggactctcg
     1981 acaccgggtt cgtcgaatcc atgggctcgc cctacctgat ggcgcacggg ctgggaactc
     2041 ctgtggcgga tgctacgacc acggtaaggc ttcccgaacc gggcgcatgg cgcgtatggg
     2101 tgcggacgtt cgactgggtt gcccgctggg atgcttccgg ctgccccggc cgcttcgaag
     2161 ttcttgttga cggcgagcct gtcggggtgg tgttcggcac ggagggcgcc gactggcact
     2221 ggcaggatgg cggggttatc cagatcggga aaccggatat cagagtatc
//
LOCUS       DAOTNG010000416         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37153_length_2267_cov_5.561031, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000416 DAOTNG010000000
VERSION     DAOTNG010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37153_length_2267_cov_5.561031"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1978
                     /locus_tag="P5179_13915"
     CDS             140..1978
                     /locus_tag="P5179_13915"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96361.1"
                     /translation="MSLHIPPEATEGAYGRITLDAKKYRPLDTVTATIHGRAQGDHAC
                     TVRVCDPEQRPYLETRVVLSENHGSVSFAAAGKLGAHYVYLIWEGETRHSRFVNFQLE
                     AETCIESGDADFDVLYAFTRDAMRQGRREYDTPDGKLVGYISADTWHLDGIWLRDWIY
                     GLPAYRWWERDMQCGLDRFLDRQEASGMLHDGIERDGRTWRVGLESDVEYIMVLAVWQ
                     TWQATGDDEWMARALPKLERALKYIRNDPLHWDEGTRLVKRQHSCDTWDFDIDGASDK
                     GNQRHVIATCDQSGYYLAFRAMAAMYRYLGLEFESEAWEGEAEEYRRRADALLWDGTK
                     YLHHHHVDEIDHADFDERDQLAMGNTWAMTRGMAQAGQARSIIEDYRRRHAETGDAYP
                     WWSLQPGYPDYLGYFKAPFLRQGGYANGGLMPWVGGELCRAAFLFGRERYGVDLLRAY
                     ADHLRRTGGVHVWYWPNGETGFRTTNEVPYASWGMAEWLNAAVEGLAGIHDDEALFRQ
                     VTLSPRWAAAGVGEVRVCARYAASKGYVAYRFRADDTKREIRFTCTGSGKAAYVRVLL
                     PEGREASSVRCGGQEMEFAIETEDVSRYVCFAMPLDGQQEIVIACE"
     gene            2022..>2267
                     /locus_tag="P5179_13920"
     CDS             2022..>2267
                     /locus_tag="P5179_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS96362.1"
                     /translation="MESHQDGLICRDECFAIQGAVFEVYRAMGCGFLEAVYQECLMRE
                     FAKRGIPYEARTALSLSYKGERLDQIYRPDFVCYGKII"
BASE COUNT          450 a          635 c          787 g          395 t
ORIGIN      
        1 agcaggcggg caggcgcttt gggagcaagg gcggcggtgc gtactatccc agaaacgaac
       61 cggtcggcgt actgtgccct gacccgtctc cgttgcccgg cgacgacgtg tgtccgaatc
      121 tgcctgtgtg ggagaactca tgagccttca catccccccc gaagcgacag aaggtgcgta
      181 cggccgcatc acgctggacg cgaagaagta ccgcccgctg gatacggtta cggcgacgat
      241 acacggccgc gcacaaggcg accacgcgtg caccgtccgc gtgtgtgatc cggaacagag
      301 gccgtatctg gaaacacggg tggtgttgag tgaaaaccac ggcagtgtgt cgttcgccgc
      361 cgcggggaag ctgggcgcgc actacgtcta tctcatctgg gagggagaaa cccggcacag
      421 ccgcttcgtg aatttccagc ttgaggccga gacgtgcatc gaatccggcg acgcggactt
      481 cgacgtgctg tatgccttca caagggacgc catgcgtcag ggccgccggg aatacgacac
      541 gccggacggg aaactggtgg ggtatatctc cgccgacaca tggcatctgg atggcatctg
      601 gttgcgtgac tggatctatg gcctgcccgc gtatcgctgg tgggagcgcg acatgcagtg
      661 cggactcgac cgcttcctcg accgccagga agccagtggc atgctgcatg acggcatcga
      721 gcgcgacggg cgaacctggc gcgtggggct ggaatcagac gtggaataca tcatggtgct
      781 ggcggtatgg cagacgtggc aggcgacggg agacgatgaa tggatggcac gggcgcttcc
      841 gaagctggag cgcgcgctga agtacatccg caacgacccg ctccactggg acgaaggcac
      901 acgccttgtg aagcgccagc acagttgcga cacgtgggat ttcgacattg acggggcgtc
      961 cgacaaaggc aaccagcgcc acgtgatcgc cacgtgcgat cagagtggat actacctcgc
     1021 tttccgcgcc atggccgcga tgtacaggta cctcgggctg gagtttgaat ccgaagcgtg
     1081 ggagggtgag gcggaggaat accgtcgccg cgcagacgcg ttgctctggg acggcacgaa
     1141 gtatctccac caccatcacg ttgacgagat agatcatgcg gattttgacg aacgcgatca
     1201 gcttgccatg ggcaacacat gggcgatgac acgcggcatg gcgcaggccg ggcaggcgcg
     1261 cagcatcatt gaggactacc ggcgccggca cgcggaaacc ggcgatgcgt acccctggtg
     1321 gagccttcag ccgggttatc ctgattacct cggctacttc aaggccccgt ttctgcggca
     1381 ggggggatac gccaacggcg gcttgatgcc gtgggttggc ggcgaactgt gccgtgcggc
     1441 gttcctgttc ggcagggagc ggtacggcgt ggatttgctc cgggcgtacg ccgatcacct
     1501 gcgtcgcacg ggcggcgtgc acgtctggta ctggccgaat ggcgaaaccg gtttccgcac
     1561 gacgaacgaa gtgccgtacg cctcgtgggg catggcggag tggctcaacg ccgccgtgga
     1621 aggtcttgcg ggaatacacg acgacgaggc gctgttccgg caggtgaccc tgtcgccccg
     1681 ctgggcagcc gccggtgtgg gcgaggtccg tgtgtgcgca cgctacgccg cctccaaggg
     1741 gtatgtcgcc taccggttcc gggcggacga cacgaagagg gaaatccgct tcacgtgcac
     1801 aggatcgggc aaggccgcct acgtgcgggt gctcctgccg gaaggacggg aagcctcgtc
     1861 agtgcggtgt ggcgggcagg aaatggagtt cgccatcgag actgaggacg tctcgcggta
     1921 cgtgtgcttc gcaatgccgc ttgatgggca gcaggagatt gtgattgcct gtgagtgagt
     1981 tgggaaccac gaaacacacg aactacacga aaggaacctg ggtggaatct caccaggacg
     2041 gcctgatttg tcgcgacgaa tgtttcgcga tacagggcgc ggtgttcgaa gtgtaccggg
     2101 caatgggctg cggttttctc gaggcagtat atcaggaatg cctgatgcgg gagttcgcga
     2161 aaagggggat tccatacgaa gcacggaccg cgttgtcgct ctcatacaaa ggggaacggc
     2221 tggaccaaat ctacagaccg gacttcgtgt gttacggcaa aatcata
//
LOCUS       DAOTNG010000417         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37553_length_2249_cov_4.583409, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000417 DAOTNG010000000
VERSION     DAOTNG010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37553_length_2249_cov_4.583409"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1066)
                     /locus_tag="P5179_13925"
     CDS             complement(5..1066)
                     /locus_tag="P5179_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRS96363.1"
                     /translation="MDNAADKPGRYMAHQYLRDRWDRTGRLLAFRAKNEEEWKVWRKD
                     LLRVLRRLTGYDTMLMCPLNPTVTETAEFDDYVRERVEIETEPGIVMPLFVLVPKQGT
                     PPYPAVIAPHGHGGGGKAAVVGLRERPEVAKAIDHYNYAYGVSFVRAGFITFCPDARG
                     FGERQEAAVKGNVLASSCSVLNNMAYPLGQTVTGMWAWDLTRLVDYIETRSDCRRDRI
                     GCAGLSGGGLQTLWATAFDERITCAVISGYLYGYKDSLLDLHQNCSCNYVPHLYEYVD
                     MGDIAATIAPRPILIETGNKDALNGARGVENVTSQVDIIRKSYGIFGVQDRVFHDVFE
                     GEHRWNGVHALPWLEKWLK"
     gene            complement(1231..>2249)
                     /locus_tag="P5179_13930"
     CDS             complement(1231..>2249)
                     /locus_tag="P5179_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96364.1"
                     /translation="TGTTYRYRLLAQDRSANRNLSVPSDTITVTPSLSDAPGAPTVFR
                     HDTTVSARPILKVTNGTANLRPVAGHLFIVARDSALTQIVTTSSVPVSGSTTEWQLPF
                     MLDHLAKYWWAVRAVDNAGFMSPFSERTSFTVDTLAKKTAVQLAAFTATGAQSHITVA
                     WRLAAASPEASFTLWRAAGENGEYERISGQVFTGGREFVFFDRAVVPGVEYRYRIGAT
                     ETGSGRSVFFGPLVARVAAPEKVVLDQNTPNPFNPVTQIAYQVPWTADVKLVIFNSLG
                     QEIRTLVNGPQSAGFYRVTWDGRTTAGGDAASGVYFARLVVTPREAGSRTAETRVIRM
                     LLIK"
BASE COUNT          434 a          727 c          640 g          448 t
ORIGIN      
        1 gtccctattt taaccacttt tcgagccacg gcaacgcgtg cacgccattc caccggtgtt
       61 caccttcaaa tacatcgtgg aaaacccggt cctgcacgcc aaaaataccg taggacttcc
      121 gaatgatgtc cacctgcgac gtcacgttct caactcccct cgctccgttg agggcgtcct
      181 tgtttccggt ttctatgagg atcggccggg gtgcgattgt cgcggcgatg tcgcccatgt
      241 ccacatactc gtacaggtgc ggcacgtagt tgcaggagca gttctggtga aggtccagca
      301 gagagtcctt gtatccgtag aggtacccgc tgatcaccgc gcaggtgatg cgctcgtcaa
      361 aggccgtcgc ccagagcgtc tgcaatccgc cacctgagag ccccgcgcac ccgatacggt
      421 ctcgcctgca atccgaacgc gtctcgatgt agtccacaag gcgcgtcagg tcccatgccc
      481 acatccccgt caccgtctgg cccagcgggt acgccatgtt gttcagcaca ctgcacgacg
      541 acgcgaggac gtttcctttc accgcggcct cctgccgctc gccgaacccg cgcgcgtcag
      601 ggcagaacgt gatgaaaccg gcgcgcacaa agctcacgcc gtaagcgtag ttgtagtggt
      661 cgatcgcctt ggcgacctcc gggcgttcgc gcagtcccac tactgcggct ttgccccctc
      721 ccccgtggcc atggggagca atcactgccg gatacggagg cgtcccctgt ttcggaacca
      781 gcacgaaaag aggcatgacg atccccggct cggtctcgat ttccactcgc tcacggacat
      841 aatcatcgaa ttccgcggtc tcggtaaccg tcggattgag gggacacatg agcatggtat
      901 cgtacccggt gagtctacgc agcaccctga gcaggtcttt tcgccatacc ttccattcct
      961 cttcattctt cgcgcgaaat gcgagcaggc gcccggtacg gtcccatcgg tcccggagat
     1021 actggtgcgc catataccgt ccaggtttgt ctgcggcgtt gtccatggag aatctcccct
     1081 ggctgaatta cggagtgaaa gaacgggttg tgcacgacat atggctaatg ataacgtccg
     1141 ggtttctgag ggagcaaacg tgttcaaatg aagggaggcg gcgttgcgcg atgcgcgcca
     1201 acgccgcctc ccggtagagg atttccgcgg ttactttatg agcagcatcc gaatcacacg
     1261 cgtttccgca gtgcgtgagc ctgcttctct tggcgtaacc accagccgtg cgaaatacac
     1321 acctgacgcc gcatcgccac cggcggttgt cctcccatcc cacgtcaccc ggtagaagcc
     1381 cgcagattgt ggcccgttca ccaatgtgcg gatctcctgc ccgagtgagt tgaagatcac
     1441 gagcttcacg tccgccgtcc atggcacctg atacgcaatc tgcgtgaccg ggttgaacgg
     1501 gttgggtgtg ttctggtcca gcaccacctt ttccggtgcc gcaacccgcg ctacgagcgg
     1561 cccgaagaac actgaccggc cacttcccgt ctcggtcgct ccgatacggt accggtattc
     1621 tacacccggt acaaccgctc gatcgaagaa gacaaactca cggccgccgg tgaacacctg
     1681 ccccgaaatc cgctcgtatt cgccattttc acctgcagcg cgccacaggg tgaacgacgc
     1741 ctccgggctc gcggcggcaa gccgccacgc caccgtgata tggctctggg cgcctgtcgc
     1801 ggtgaatgcc gccagttgaa ccgcggtctt cttggcaagc gtatccaccg taaagctcgt
     1861 ccgctcggag aacggactca tgaagcccgc attgtccacc gcccggacgg cccaccagta
     1921 tttcgccagg tgatccagca tgaatggaag ctgccattcc gtcgtcgaac cgctcaccgg
     1981 gacggaactg gtggttacaa tctgcgtgag ggcggaatcg cgggcaacga tgaacagatg
     2041 ccccgccacc ggccgcagat tcgccgtgcc gttggtcact ttcaggatag gacgagcgct
     2101 cacggtcgta tcgtgccgga acaccgttgg tgcgcccggc gcatccgaaa gagacggcgt
     2161 gacggtaatc gtatcgctcg gaacactgag attgcggttg gcggaacggt cctgtgccag
     2221 gagccgatac cggtaggtag tgccggtca
//
LOCUS       DAOTNG010000418         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37586_length_2248_cov_3.980392, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000418 DAOTNG010000000
VERSION     DAOTNG010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37586_length_2248_cov_3.980392"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5179_13935"
     CDS             <1..239
                     /locus_tag="P5179_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96365.1"
                     /translation="PLEIQTWVISPVETWGKTVGHYALLEVSPRSEGRTIPGRHTETI
                     RFRVSGANGRIPKFWAWVKLACNGKPAYLPVPER"
     gene            319..1572
                     /gene="hydF"
                     /locus_tag="P5179_13940"
     CDS             319..1572
                     /gene="hydF"
                     /locus_tag="P5179_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRS96366.1"
                     /translation="MQTTPKSMRLHIGLFGRRNVGKSSILNALTRQDVSIVSPEAGTT
                     TDPVEKAMELLPLGPVLFIDTAGIDDVGALGEKRIAKTTQVFDRTDLAVVITDQPSPG
                     EFENRLIEEFAKRNTPVVFVFNKADLHRATPVALKDPQGRAIPTVTTSVTTGQGISEL
                     LKALITTAPEDFVSTPPIIGDLLPAGELAVLVVPIDKEAPKGRLILPQVQTIRDILDT
                     DAYCIVVKERELRDALERLTRPPKIVVTDSQAFQKVAADVPPSVWLTSFSILFARLKG
                     DLPELVRGALAIESLQGGDKVLVCEACTHHPIQDDIGRVKIPRWLTQYVGATIEFVHY
                     QGHDFPSDLAPYRLIIHCGGCVHNRREMLTRIALAKRAGVPITNYGLSIAYTLGIFER
                     ALEPFPAAKEVIAAHRQEVTGGKTG"
     gene            complement(1714..>2248)
                     /locus_tag="P5179_13945"
     CDS             complement(1714..>2248)
                     /locus_tag="P5179_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS96367.1"
                     /translation="PEEVRRNVEGSKEYIKLAADVGSPGIKMRPNNAPDEVPLAATLE
                     QIGRALREVAQFGSDYGVEVRLEVHGRITCNPPNIRAILDHADHRNLKACWNSNDSDM
                     GPDGTIRQNFNLLKHDIGLVHMTDLANPGYPWRELFSGLRSIGYKGFCLAEVPESAEP
                     LRFMRYYRALWMELCNP"
BASE COUNT          480 a          680 c          634 g          454 t
ORIGIN      
        1 agcctctgga gatacagacc tgggttatct cgcccgtcga aacgtgggga aaaacggtcg
       61 ggcactacgc gcttctggaa gtttcaccgc ggtccgaagg gcggacaatc cccgggcggc
      121 acacagagac gatccgtttc cgggtttcgg gtgccaatgg tcgcattccg aaattctggg
      181 cctgggtcaa acttgcgtgc aacgggaaac cagcatacct gccggtgccg gagagatgac
      241 tctcgcgttc gcccggcggg gtaccttcat cggaccccac ggccgcgcgc actaatcgac
      301 acaaaggaga cgcttgaatt gcaaaccacg ccaaaaagca tgcggctgca tatcggcctg
      361 ttcggtcgga ggaacgtagg gaaatcgagc attcttaacg cgttgacacg gcaggacgtg
      421 tctatcgtca gtccggaggc cggcaccacc actgacccgg tggaaaaagc aatggagttg
      481 cttcccctgg gaccggtcct tttcatcgat acggccggca tagacgacgt aggcgctctg
      541 ggcgaaaaaa ggatcgccaa aacgacacaa gtattcgacc gcaccgacct cgctgtcgtc
      601 atcactgatc aaccgagccc cggcgaattt gaaaaccgcc tcatcgagga gtttgcgaaa
      661 cggaacaccc cggtggtttt cgtattcaac aaagccgatc tacatcgcgc gactcccgtc
      721 gcgctgaagg accctcaggg acgtgcgatt ccgacagtca cgacgtccgt aacaacaggg
      781 cagggtattt ctgaactgct gaaggcactc attactactg cgccggagga ttttgtcagc
      841 accccgccga tcatagggga ccttctgccg gccggcgaac tggcggtgct ggtcgtaccg
      901 atcgacaagg aagctccgaa agggcgactc atcctccccc aggttcaaac aatccgggac
      961 attcttgaca ccgacgccta ctgcattgtc gtgaaggaaa gggagctgcg ggatgctctg
     1021 gagcgtctca cgcgcccccc gaaaatcgtt gtcaccgact cgcaggcgtt tcagaaggtc
     1081 gcagcggatg tgcctccgtc ggtctggctc accagctttt cgattctgtt cgcgcgtctg
     1141 aagggtgact tgccggaact cgttcgaggt gcgcttgcca tcgagagcct gcaaggcgga
     1201 gacaaggttt tggtctgcga agcgtgcact caccacccga ttcaggatga catcggcaga
     1261 gtcaagatcc cacgatggct tacccagtac gtgggggcca ccattgaatt cgttcattat
     1321 cagggacacg attttccctc tgatctcgca ccttaccggc tgatcattca ttgcggcggg
     1381 tgtgttcaca acaggcggga gatgcttaca agaatcgccc tcgcaaagcg ggcgggcgtt
     1441 cccattacga attacggcct ttcaatcgca tacacgctcg ggatcttcga gcgggcactc
     1501 gaacccttcc ctgcggcaaa ggaagtcatc gccgcgcaca gacaggaggt cacgggcggc
     1561 aagaccggct aaggcccttc ctgcttgctt tgccctccgc agggcagacc tgacaagcct
     1621 ctttttgagc gcaacagaca aatcagaagc ggcgagtgga agcccgtctt ggctcccact
     1681 cgccgcccgt tcctgttcaa ccagcgttcg cttttacggg ttgcaaagct ccatccacaa
     1741 ggcccggtaa tacctcatga acctgagcgg ctcagcactt tcgggaactt ctgcgaggca
     1801 gaatcccttg tagccgatgg aacgcaggcc agaaaaaagc tcacgccagg ggtacccggg
     1861 gttcgcgagg tcggtcatgt ggacgagacc tatgtcgtgc ttcagaaggt tgaagttctg
     1921 gcgaatcgtc ccgtccggac ccatatcgct gtcgttggag ttccagcacg ccttcaagtt
     1981 cctgtgatcc gcgtggtcaa ggatggcgcg gatgttcggc ggattgcagg taatcctccc
     2041 gtgcacttcc agtcgaactt caacgccata atcggaaccg aactgggcca cttctcgaag
     2101 cgcccgcccg atctgttcca gcgtcgccgc gagcggaact tcatccggtg cattgttcgg
     2161 acgcattttg atacccggcg agcccacatc ggccgcaagt ttgatgtact ccttgctccc
     2221 ctccacattc ctccgcactt cctcaggg
//
LOCUS       DAOTNG010000419         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37703_length_2243_cov_3.419104, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000419 DAOTNG010000000
VERSION     DAOTNG010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37703_length_2243_cov_3.419104"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="P5179_13950"
     CDS             <1..1738
                     /locus_tag="P5179_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRS96368.1"
                     /translation="NRLPGRNVWEIFLPEERLQHGQFYRLEMAWDGGRGERIPAYARR
                     VVQNPETLVFAAQVWDPEPYVWRNPDFRVPDRVPLIYEAHVGMAQEREGVGGYTEFRE
                     RILPRIKRAGYNTVQLMAVMEHPYYGSFGYHVSSYFACSSRFGTPEELKALVDAAHGE
                     GLAVIMDIVHSHAVKNEREGLSRFDGTLYQYFHDGLRGWHEAWDSRCFDYGKTDVLHF
                     LLSNCRYWLDEFRFDGFRFDGVTSMLYLHHGLGVDFMDYSQYFDATVDEDAWIYCRLA
                     NRVIHQVRPDAMTVAEDVSGMPGLAAPLEDDGAGFDYRMAMGVPECWFRLVRDVRDED
                     WSIGYLWHELTNRRADERTVNYVESHDQALVGGKTLFFQLVDAAIYDAMLRSMRHLEV
                     ERGVALHKLARLATLATAGHGYLNFMGNEFGHPEWIDFPREGNGFSYRYARRQWTLRD
                     NPELLYWCLGEFDAAMLEMVTRYRALEGTLPRRLFVSDQEKILVFERGPLVFLFNFHT
                     HTSVADFKVLVPPGSYHWVMDSDEERFGGQGRIAAGQSFALIAEQRDRELCQTLRVYL
                     PCRTAMVLERRI"
     gene            complement(1755..>2243)
                     /locus_tag="P5179_13955"
     CDS             complement(1755..>2243)
                     /locus_tag="P5179_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRS96369.1"
                     /translation="GHVLFASGWDRFLFLQIGALNAMLFVFNLLPAFPMDGGRMLRAI
                     LQQFFMTRLRATWVASRIGRFLALLLGLSVLYSLFTGRFDGFKLIRLLIAYFIYQAAE
                     GEYRMVLAEERAGRATSPFDGFPFMGGRSSAMPPPDDGKAVVSPPPYERRGATRVDVH
                     KT"
BASE COUNT          416 a          616 c          758 g          453 t
ORIGIN      
        1 gaaccgtttg cccggacgca atgtctggga gatcttcttg ccggaagagc gtttgcaaca
       61 tggccagttt tatcgtctcg agatggcgtg ggacggcgga cgcggtgagc gcattccggc
      121 ttacgcgcgc cgggtggtgc agaatcccga gaccttggtg ttcgcggccc aggtgtggga
      181 tccggagccg tacgtctggc gcaatcccga cttccgggta ccggaccggg tgccgctgat
      241 ctacgaggcg catgtcggaa tggcgcaaga gcgggagggg gtcggcggct acacggagtt
      301 ccgggagcgg attctgccgc ggatcaagcg ggccggatac aacacggtcc aactcatggc
      361 ggtgatggag catccgtatt acggctcgtt cgggtatcat gtgtcgagct acttcgcctg
      421 ttcgtcccgt ttcggcaccc ccgaggaatt gaaagcgctg gtggatgcgg cgcacgggga
      481 agggcttgcc gtgatcatgg atatcgtgca ttcccacgcg gtcaaaaacg agcgcgaggg
      541 gctgtcgcgt ttcgacggca ccctctacca gtatttccat gacggcttgc gcggttggca
      601 tgaggcgtgg gattcgcgct gtttcgatta cggtaaaacc gatgtgctgc actttctgct
      661 ctccaactgc cggtactggc tggatgagtt ccgcttcgac ggattccgtt ttgacggggt
      721 caccagcatg ttgtatctgc accacgggct cggagtggat tttatggatt acagccagta
      781 tttcgatgcc acggtggacg aggacgcgtg gatctactgt cggctcgcga accgcgtgat
      841 ccatcaggtg cggccggatg cgatgaccgt ggccgaagat gtgagcggca tgccgggcct
      901 tgccgcaccg ctggaggatg acggcgcggg attcgattac cggatggcca tgggggttcc
      961 cgagtgctgg ttccgtttgg tgcgcgatgt gcgggatgag gattggagca ttggatacct
     1021 gtggcacgaa ttgaccaacc ggcgcgcgga tgagcggacg gttaactacg tggaaagcca
     1081 cgatcaggcg ctggtgggcg ggaaaaccct tttcttccaa ttggttgacg cggcaatcta
     1141 tgatgccatg ctgcgttcga tgcggcatct cgaggtggaa cgcggtgtgg ccctgcacaa
     1201 attggcgcgg ttggccacgc tggcgaccgc tggccacggg tatctcaatt tcatgggtaa
     1261 tgagttcggc catcccgagt ggatcgattt cccgcgcgaa ggtaacggat tctcctaccg
     1321 gtatgcgcgt cggcagtgga cgttgcgcga caacccggag ctcctctact ggtgtctggg
     1381 cgagtttgat gcggcgatgc tggagatggt cacgcggtac cgggcgctcg aagggaccct
     1441 gccgcgtcgg ttgttcgttt ccgaccagga gaagattttg gtgttcgaac gagggccgct
     1501 ggtcttcctc ttcaacttcc acacccacac ttcggttgcc gatttcaagg tgttggtgcc
     1561 gcccggttcc tatcactggg tgatggacag cgatgaagag cgtttcggag gccagggccg
     1621 gatcgcggca gggcagtcct ttgcgctgat cgccgaacaa cgtgaccggg agttatgcca
     1681 gaccctccgc gtctacctgc cctgccggac cgcgatggtg ctggagcgca ggatctgaaa
     1741 gatccgaacc gcggtcacgt cttgtgcaca tcgacgcggg ttgcgccgcg ccgctcatat
     1801 ggcgggggac tcaccactgc cttgccgtca tccggcggcg gcatggcgga ggatcggcct
     1861 cccatgaagg ggaagccgtc aaacggggag gtcgcgcgtc ccgcgcgctc ttccgccaga
     1921 accatgcggt attcgccttc tgcggcctga tagatgaaat aagcgatcag caggcggatc
     1981 agcttgaatc cgtcgaagcg gccggtgaac agtgagtaca gcaccgagag tcccagcaac
     2041 agggcgagga aacgtccgat gcgcgaggcg acccaagtgg cgcgaaggcg cgtcatgaaa
     2101 aattgctgca ggatcgcgcg tagcatgcgt ccgccatcca tcgggaaagc aggcaacaga
     2161 ttgaagacga agagcatcgc attcaacgcg ccgatttgca ggaagaggaa ccggtcccaa
     2221 ccgctcgcaa acagcacgtg gcc
//
LOCUS       DAOTNG010000420         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37946_length_2233_cov_5.211662, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000420 DAOTNG010000000
VERSION     DAOTNG010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37946_length_2233_cov_5.211662"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="P5179_13960"
     CDS             complement(<1..977)
                     /locus_tag="P5179_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96370.1"
                     /translation="MAGTSVALITMADGPHAGAYLGTFSVMKRVDSVHVCDSSGRIFD
                     QAAQLLGKKLVGTSTDVEACLNQGPYAFVLVALDNFSAPAVISQVLDAGFHVFSEKTG
                     ARTAAEWRPLKEKAAKKHLLLGMSYPNRYRPSVQEARRILQAGYLGRLYGFYVQSVAT
                     QAVLRDPKGSWMFRFDRAGGGYVIWLGCHYLDLLRYVTNEEVTSVAAQYGRVSPVEFD
                     VEDAVGLAMKLDSGAVGAANFGYYLGPGKIAAGKQSQLTFWGELGWMKLQPGDDETAP
                     LEVYSDHPEFLGTPYRKTFFTHSDVPKAYGSGWGLAFLNDFLAACLGEK"
     gene            1163..2077
                     /locus_tag="P5179_13965"
     CDS             1163..2077
                     /locus_tag="P5179_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS96371.1"
                     /translation="MVDLHLHTACSDGREAPEIVVYRASQAGLSAIAVTDHDTVDGSI
                     RAVAAAPDHMVVLSGIELSAAEGESAIHVLGYGIDPFDDALRVLLESMKRDRYVRAGE
                     IVNRLHAMDVPLTLESVEHVAAGSPITRAHVAEALRREQMVATYTEAFERYLSDAQPA
                     FVLQESLSASEAIAAIHRAGGVAFMAHPGVTRRDEIIPSLVKAGLDGIEVYHPNHESA
                     AERFYLNLAEKHGILVSGGSDYHASFRGTDRMGTPYVPREVFDRIVEAIDLRAGTRGS
                     GDEEDSDDGSLIGPPPGARAEAPGPQSG"
BASE COUNT          396 a          757 c          671 g          409 t
ORIGIN      
        1 tccttttcgc cgaggcacgc cgcgaggaaa tcgttgagga atgcaagtcc ccagccgctg
       61 ccgtacgctt tcggcacgtc actgtgcgtg aaaaatgtct tccggtacgg cgttccgagg
      121 aactccggat gatcggaata gacctccagc ggcgcggttt cgtcgtcgcc gggctggagc
      181 ttcatccacc ccagctcgcc ccagaaagtg agctgtgatt gcttgcccgc cgcgattttc
      241 cccgggccga ggtagtaccc gaagtttgcc gcacccacgg cgccgctgtc aagtttcatg
      301 gcaaggccca ccgcgtcctc cacatcgaac tccaccgggc ttacccggcc atactgggcg
      361 gccacgctgg ttacctcctc gttcgtcaca taccgcagca ggtcgaggta atggcaaccc
      421 agccagatca cgtacccacc gcccgcccgg tcgaaccgga acatccagct gcccttcggg
      481 tcgcgcagga ctgcctgcgt ggccactgac tgcacgtaga acccgtacaa tcgcccgaga
      541 taccccgcct gaaggatgcg tcgcgcctcc tgcacgctcg gtcggtaccg gttcggatac
      601 gacatcccca gcaggaggtg tttcttcgct gctttttctt tgagcggcct ccactccgcc
      661 gcggtccgcg cgccggtttt ttcgctgaac acgtggaagc ccgcatcgag cacctgcgaa
      721 atgaccgcgg gagcggagaa gttatccagc gccaccagca caaaggcgta cggcccctga
      781 ttcaggcacg cctccacatc cgtggaagtg ccgaccagct ttttcccgag aagctgggcc
      841 gcctggtcga aaattctccc gctggaatcg cacacgtgca cggaatccac ccgtttcatc
      901 actgaaaacg tgccgagata ggcgcccgca tgggggccat cggccatggt gataagggcg
      961 actgaagtgc cggccatgga atcctccctg ggaaaaacgt ggtgtgcatg taagaaaacg
     1021 ctctcccgcg ctctgacaca aggacacgcc ccgctccgcg cctggggcag acctgcgccc
     1081 gcgttgacgg agtgcgcgcg cgcacgtact ttcgttaacc tctgaaatcg gcccttcccc
     1141 gcgccgacga aaggcgtacg tcgtggttga tctgcacctc catacggcct gctccgatgg
     1201 ccgggaagca ccggaaatcg tcgtgtaccg cgccagccag gccggtctat cggccatcgc
     1261 ggttaccgac cacgatacgg tggacggttc catccgggca gtcgccgccg ctccggacca
     1321 catggtggtc ctttcgggga tcgagctgag cgcggccgaa ggggaatcgg ccatccatgt
     1381 cctcgggtac ggcatcgacc ctttcgacga cgccctccgt gtgttgctgg agagcatgaa
     1441 acgcgaccgt tatgtccgcg ccggggagat cgtcaaccgg ctccacgcta tggatgtgcc
     1501 tctcacgctg gaatccgtgg agcacgtggc ggcgggctcg ccgatcaccc gggcgcacgt
     1561 tgcggaggcg ttgagaagag agcagatggt ggccacgtat accgaggcgt tcgagcgtta
     1621 cctttccgac gcgcaaccgg cgttcgtgct gcaggaaagc ctctccgctt ccgaagcgat
     1681 tgccgccatc caccgcgccg gcggcgtggc attcatggcc catcccgggg ttacccgccg
     1741 cgatgaaatc atcccgtcac tcgtcaaagc cgggctggac ggtatcgaag tctatcaccc
     1801 gaatcatgaa tcggcggcgg aacggtttta cctcaatctt gccgagaaac acggcatcct
     1861 cgtttccggc gggtccgact accacgcctc tttccgggga accgatcgaa tgggaacgcc
     1921 gtatgtaccg cgggaagtgt tcgatcgtat cgtggaagcc attgacctcc gcgcgggcac
     1981 ccggggttcc ggagacgaag aggattccga cgacggctcc ctcatcggac ccccacccgg
     2041 cgcacgtgcc gaggcacccg gcccgcaatc cgggtgaccg gcaggagctc tgcgttcgtg
     2101 gcaacaatcg cgtacagccg taccgttctt gttcccgccc tgctctctct gctctgcgcg
     2161 cgggccggcg catcttcccc cgcggactcg gtttcgcgcg ctctgcgtgc aggccggtgg
     2221 caggtggcgg agc
//
LOCUS       DAOTNG010000421         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_37962_length_2232_cov_5.681213, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000421 DAOTNG010000000
VERSION     DAOTNG010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37962_length_2232_cov_5.681213"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..503)
                     /locus_tag="P5179_13970"
     CDS             complement(171..503)
                     /locus_tag="P5179_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96372.1"
                     /translation="MNALVTINAGICGFVTSAEVSSPDNQNVEFHVSSNCEKIAKLGK
                     SLKQCEPIDAYQEISPASDSVFMAIVRDNLKGCCSGCAVPSGLFKAMQVAAGLALPKD
                     ISISLSKR"
     gene            complement(570..1019)
                     /locus_tag="P5179_13975"
     CDS             complement(570..1019)
                     /locus_tag="P5179_13975"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRS96373.1"
                     /translation="MKPKLKILFLCTGNSCRSQMAEGWTRYLKGDEIEPFSAGIETHG
                     LNPRAVRVMAEAGVDISGHRSKHVDELRDVAFDFVVTVCDHAHESCPMWLGGKARVVH
                     VGFDDPPRLAQNSETEEEALSHYRRVRDEIREFVETLPAALKKLTPG"
     gene            complement(1004..1369)
                     /locus_tag="P5179_13980"
     CDS             complement(1004..1369)
                     /locus_tag="P5179_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS96374.1"
                     /translation="MRDLLAITKALADSNRIRILAMLRAGELCVCQIVEVLGLAPSTV
                     STHLSILYGAKLVESSKRGRWVYYRLPDPRSSVLVNDCIEWVIRSVENDETIEEDRVK
                     VARVISVCPEELCRTSNQS"
     gene            1515..1850
                     /locus_tag="P5179_13985"
     CDS             1515..1850
                     /locus_tag="P5179_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS96375.1"
                     /translation="MQMLTLNNAQAQARYEARARIIKALAHPTRLFIVDVLAQQSHCV
                     CELTAMVGADTSTVSKHLSLLKDAGLVSDEKRGTQVYYHLVAPCALRFLDCAEAVMKV
                     RLEKQLAAV"
     gene            1907..>2232
                     /locus_tag="P5179_13990"
     CDS             1907..>2232
                     /locus_tag="P5179_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRS96376.1"
                     /translation="MNWKDEWKPLALILAIFFAAFYLPVGIPRFDNAVSEAFQLTKWY
                     AREHVVLCLIPAFFIAGAISVFVNQAAVMRYLGAKAKRTLAYGVASVSGTILAVCSCT
                     VLPLFAG"
BASE COUNT          480 a          640 c          602 g          510 t
ORIGIN      
        1 cgttgcgtca gctttctccc cgggtgttcc ggaacaacac gcagagctcg agcaacagga
       61 tgtggcgccg gaagtcgcga tttcggcgta acgcttgcgg atcttgcccc gcgtaatgtg
      121 tatgagttca gacacgaaag tccctcctgg aaccaggctc ccgtcgcttc ctatcgtttg
      181 ctcagtgaga tggatatgtc cttcggcagg gcgagcccgg cggcgacctg catcgctttg
      241 aacaggccgg acgggacggc gcatccggaa cagcaccctt tcagattgtc ccttactatc
      301 gccatgaaca cgctgtcgga agcggggctt atctcctggt acgcgtcgat cggctcgcat
      361 tgttttagag atttccccag cttggcgatc ttttcgcagt tgctcgatac atggaactcc
      421 acattctggt tgtccgggct ggacacctcc gcagaagtga caaagccaca gattcccgca
      481 ttgatcgtca ccaacgcatt caaatcgcac ctcatgtcgt gtggaaggga aaatcacacg
      541 gagtactcag ctccgcagct caaattcaat caacccggtg tgagtttttt gagggcagcg
      601 ggtaacgttt cgacgaactc ccggatctcg tcgcgcaccc gccggtaatg actgagcgct
      661 tcctcttccg tctccgaatt ctgtgcgaga cggggcgggt catcgaatcc cacgtggacc
      721 acacgcgctt tgccgccaag ccacatgggg caggactcat gcgcgtgatc gcacacggtt
      781 acaacaaaat cgaacgcgac atcccgaagc tcatcgacgt gtttggagcg atggcccgaa
      841 atatctaccc cggcttcggc catgacacgc acggcacggg ggttgagccc gtgcgtttcg
      901 atacccgctg aaaagggttc tatttcgtca cccttgaggt atcgcgtcca tccttccgcc
      961 atctggctcc tgcacgaatt gcccgtacac aggaacagga ttttcagctt tggtttgatg
     1021 ttcggcacag ttcctccgga cagacggaaa tgacgcgggc caccttaacc cggtcttcct
     1081 cgatggtttc gtcgttctca acggaacgga tcacccattc gatgcaatcg ttgacaagca
     1141 cacttgaacg ggggtctggc aggcggtagt acacccatcg gccgcgcttg ctgctttcga
     1201 ccagcttcgc cccgtacaga atggaaaggt gggtggagac agtagatggt gcgagaccaa
     1261 gaacctcaac aatctgacag acacagagct cgcccgcacg cagcatcgcg agaatcctga
     1321 ttcggttgct gtccgcaagt gctttcgtga tagcgagcag gtcacgcatt gcaattcctc
     1381 atttcggcat ataacgaatt ataccgcacc gagcctgctg acgcaagagc acgaaagaaa
     1441 tcaatccccg atttggggtg cctcttgaca atccttggcg aattcgccaa atttagaaag
     1501 tgaaaggaac gcacatgcag atgctcaccc tgaataacgc acaggctcag gcccggtacg
     1561 aggcacgcgc cagaatcatc aaggccctcg cgcaccccac gcgcctgttc atcgtagacg
     1621 tactcgcgca gcaatcccat tgcgtgtgtg aactgaccgc gatggttggc gcggatacgt
     1681 ccacggtctc caaacacctc agtttgttga aagacgccgg ccttgtttca gacgagaaaa
     1741 ggggcaccca ggtgtattac caccttgtgg caccctgtgc gctccggttt ctcgactgtg
     1801 ccgaagcagt aatgaaagtt cgtctggaga agcaattagc cgccgtctga gtttttttgt
     1861 tgtgtttctt ggttattttg ccacatagtg aaaaagaagg tccctcatga attggaaaga
     1921 tgaatggaaa cctctggctc tgatcctcgc catcttcttc gcggcgtttt acctgcccgt
     1981 tgggatccca cgatttgata acgcggtatc agaggcattt caactcacaa aatggtacgc
     2041 gcgggagcac gttgttctgt gcctgattcc ggcgttcttc attgcagggg cgatttccgt
     2101 cttcgtgaac caggccgcgg tgatgagata cctcggcgcg aaggcgaaga ggactcttgc
     2161 gtacggagtt gccagtgtct ccggcacgat cctcgcagta tgctcatgca cggttctccc
     2221 gttgttcgcg gg
//
LOCUS       DAOTNG010000422         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38002_length_2230_cov_6.022989, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000422 DAOTNG010000000
VERSION     DAOTNG010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38002_length_2230_cov_6.022989"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="P5179_13995"
     CDS             <1..1951
                     /locus_tag="P5179_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRS96377.1"
                     /translation="AERLLNASETSPDTNLSRMRRGLIYLATNRLREADSVLAAAELP
                     ASLAPYREYWRAQAWQRTGNLDQAWAAFDRLAGAGIPAISDSAQFAALQVATAAGNRQ
                     RILRAATRLTERSDNLAAAGWLSLMETDSARWRGAWDALRTRYPRTVEAYRAAEQAQQ
                     RGWQPSGADKLALADIYSRRGQVATAMRWWNDALQDPALASRRNEIRFTAAEYLVNRR
                     QLDDAETYLTALIADSSGNSFLPRALRLYASLHLKANNEAQMRAWERTFVERFPRHSQ
                     VPAALLAIGMSWEREGDFSSAIATYEEIINRFPDNAQTEEARWRLGFCQFRLGRYDAA
                     RHAFSPLARTARDFVIKDQAGYWLALALKAEGQEDSARTIWRRTAEFSPRSFYAVASA
                     VACGSPVVKDAREPLQASPPNRARISAWNGFPEAEWLALLGQWRFARAALEQRIVGDA
                     NTIAEMESAADAFEWLADYGTSLRWRWKAMWKRTTDDRHHELAPELLARVYPDFFHAD
                     VVAAAQEHAIEPALVWAIIRQESVFDPEARSGADARGLMQIIPSTGAKLAREEGLSGY
                     TAQDLYDPGLNIRLGTRYAAQQLRQFGSIDLMAAAYNAGPHNAVRWKLDDPDVQREAI
                     SFGETRKYVKLVLRNYYVYKALYGD"
BASE COUNT          388 a          759 c          715 g          368 t
ORIGIN      
        1 ggcggagcgc cttctgaacg cctccgagac ctctcccgac acgaatctct cgcggatgcg
       61 ccgtggactc atctacctcg cgacgaaccg cctgcgcgaa gcggactctg ttcttgccgc
      121 agcggagctt cccgcctcac tggcgccgta ccgggagtac tggcgcgccc aggcatggca
      181 gcgcacgggc aacctcgatc aggcgtgggc ggccttcgac cgcctcgcgg gagccggtat
      241 acctgccatc agtgattccg cccagttcgc cgcgttgcag gtggccaccg cggcgggcaa
      301 caggcaacgg atcctgcgtg cggcaacccg tctgacggaa cggagcgaca atctcgcggc
      361 cgcagggtgg ctatccctta tggagacgga ctccgcgcgg tggcgcgggg catgggatgc
      421 gctcagaacc cggtacccgc gcaccgttga ggcgtaccgc gcagcggaac aggcgcaaca
      481 acggggatgg cagccctccg gcgccgataa gctggcgctg gccgacattt actcccggcg
      541 cgggcaggtt gccacggcca tgcgctggtg gaatgatgcg cttcaggacc ccgcccttgc
      601 gagccggcgc aacgagatac ggttcaccgc cgcggagtac cttgtcaacc ggcgccagct
      661 tgacgacgcc gagacgtatc tgaccgcgct cattgccgat tcgagcggga attcattcct
      721 gcctcgcgcg ttgcgcctgt acgcatcgct gcacctgaag gcgaataacg aggcgcagat
      781 gcgcgcgtgg gagcgcacgt tcgtggaacg tttcccgcgg cacagccagg ttcccgccgc
      841 ccttctcgcc atcggcatga gctgggagcg ggagggcgat ttctcctccg ccatcgcgac
      901 gtacgaagag atcatcaacc ggttccccga caacgcgcag accgaggaag cgcgatggcg
      961 gcttggcttc tgtcagttcc gccttgggcg ctacgatgcc gcccggcacg ccttttcccc
     1021 tctcgctcgc accgcacggg attttgtcat caaggatcag gcgggttact ggcttgcgct
     1081 ggccctgaag gcggaagggc aggaagactc cgctcgtacc atctggcggc gcacggcgga
     1141 gttctccccc cggagctttt acgccgtcgc atccgcggtt gcgtgcggct cgccagtggt
     1201 gaaggatgca cgggagcctc tgcaggcatc cccgccgaat cgcgcgcgca tcagtgcctg
     1261 gaacggattt ccagaagcgg aatggctggc cctcctcggc cagtggcgct ttgcacgggc
     1321 agcgctggag cagcgcattg tgggcgacgc gaacaccatc gctgaaatgg aatcggcagc
     1381 cgatgcattc gaatggcttg ccgattacgg aacctccctc agatggcgct ggaaagccat
     1441 gtggaaacgt accaccgacg accgacacca cgagcttgcg cccgagcttc tcgcacgggt
     1501 ctacccggac ttctttcacg cggacgtcgt cgcggcggcg caggaacacg caatagagcc
     1561 cgcgctcgtc tgggcgatca tccggcagga aagcgtgttt gaccctgagg cgcggtcagg
     1621 ggcggatgcc cgcggcctga tgcagatcat cccgtcgacg ggcgcaaagc tggcgcggga
     1681 agaaggcctc tcggggtaca ccgcgcagga cctctacgac cccggcctca acatccgcct
     1741 cggcacgcgg tatgccgcgc aacagctccg ccagttcggc agcatcgacc tcatggccgc
     1801 cgcctacaac gcgggcccgc acaacgccgt gcggtggaaa ctggacgacc ccgacgttca
     1861 gcgcgaggcc atctccttcg gggaaacgcg gaaatacgtg aagctggtgc tcaggaacta
     1921 ctacgtctac aaggccctct acggcgattg acccacgccc cttcgcgttt ttcgcggctt
     1981 cgcgtgcccc tcccgacctc gactattgac cctgcggttt gtttccggta tcaattggcg
     2041 catacacgag aggacggtgt tcccctccgc gttcgacgcc ttcccgcgag atcccaaaag
     2101 gagacatcgc gatgaacgca cgcaaagaga acgtctgcac gttggtggcc atcctgctca
     2161 ccggcgcagc gcacgccggg gccggcacgt ggacttcggg cgcgcctctg ccgtcggcgc
     2221 gacagggcgc
//
LOCUS       DAOTNG010000423         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38107_length_2225_cov_4.169124, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000423 DAOTNG010000000
VERSION     DAOTNG010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38107_length_2225_cov_4.169124"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5179_14000"
     CDS             <1..237
                     /locus_tag="P5179_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96378.1"
                     /translation="PVWVVSDSRGVAFALSRASYIGGEAAPVAWAPAVVFPSAEALKH
                     ARETGGDRNQTVKCPFCGEHLTLPMHFGDRNSRH"
     gene            260..553
                     /locus_tag="P5179_14005"
     CDS             260..553
                     /locus_tag="P5179_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96379.1"
                     /translation="MSSESNNKENPAPSTRTTGGIAGWIRRLVWRRNARRMKESDIDL
                     MRKRIIIITSQLQQLPDSSTVLRRRKELLRDRERCEFVIAEWERRYDRPKDSR"
     gene            649..724
                     /locus_tag="P5179_14010"
     tRNA            649..724
                     /locus_tag="P5179_14010"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:682..684,aa:Cys,seq:gca)
     gene            825..1904
                     /locus_tag="P5179_14015"
     CDS             825..1904
                     /locus_tag="P5179_14015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS96380.1"
                     /translation="METLPDTVDGPRGGTWYEFGEILDFLNFVFRSSVGRKPSIGGDY
                     PHLYNERNVHNFRHIRVNGRVISNVSIYPARVQWGDAVLKIGGIGGVATDPDYRKHGY
                     AGTILAHCLEYMDRELDCDLSILWTGIPDYYRRWGWEHAGQNWTFFIDRTTITYLPVA
                     PTGEVLTDTLDPRVIQAVESLHAARRAGVVRDRELTEVLLNIRTRVRCAVLMDGETPV
                     AYIVYSPGEQIDVKDCGGDGSAVLGLLRVVFAQCGARTAQIHTPAQDQSVAPLFIERG
                     FAVRANYSGMLAVLKPQRILEAYGIRDVTVRPEGDGWQIQTSTASVFLRKPDLVKLLF
                     GPERPPEIGHSKLPLPFYYGQLDHM"
     gene            2023..>2225
                     /locus_tag="P5179_14020"
     CDS             2023..>2225
                     /locus_tag="P5179_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRS96381.1"
                     /translation="MAAFRAAVALGVDVIEIDVHLSRDGHVVIMHDAKLDRTTDGKGP
                     IAEHTLAEIKRVDAGSKKGAQFAG"
BASE COUNT          447 a          664 c          693 g          421 t
ORIGIN      
        1 ccggtatggg tagtttctga ttcccgcggc gtcgcgttcg cgctctcccg cgcaagttac
       61 atcggtggtg aagcagcgcc cgttgcctgg gcgccggcag tggtgtttcc gagcgcggaa
      121 gccctgaaac acgcgcggga aacgggcggt gaccggaacc aaaccgtcaa atgccccttc
      181 tgcggcgaac acctcaccct tcccatgcat tttggagaca ggaactcccg tcactagaac
      241 cagaggaata catgcgcata tgagcagcga gtcgaacaac aaagagaatc cggctccttc
      301 gacacgaacc acagggggta tcgcgggatg gatcagacgc cttgtgtggc gccgaaatgc
      361 ccgcaggatg aaggaatccg acatcgacct gatgcgcaaa cggatcataa tcattaccag
      421 tcaactccaa cagcttcccg actcctccac ggtcctgaga cggcggaagg aattgctgcg
      481 ggaccgagag cgctgcgagt ttgtcatcgc ggaatgggag aggcggtatg accgcccgaa
      541 ggactcacgg tgaaggactt ctcccggtgg ttggtcgtca ccacggcagc gccggggcag
      601 ccgcaggtct tgatacgctt ttccgtcgcg cgtacattgt aagaatccgg cggcgtagcc
      661 aaggggctaa ggcggaggtc tgcaaaacct ctattcggcg gttcgagtcc gcccgccgcc
      721 tccacgtttt ccgaagggac gggcgcgatc gcagggcctg tccctttttc tttgcgcttc
      781 cggcaacgtg gcacctgaag ccattctgag aggagaattg cccgatggag acgctccccg
      841 acaccgttga tggcccacgc ggcggaacgt ggtacgaatt cggcgagatt ctcgacttcc
      901 tcaatttcgt gttccgctcc agtgtcggcc gcaagccaag catcggcggg gattacccgc
      961 acctgtacaa cgagcggaac gtgcacaatt tccggcacat ccgcgtgaac gggcgcgtca
     1021 tcagcaacgt gagcatctac cccgcccggg ttcaatgggg tgatgccgtg ctgaagatcg
     1081 gcggaatcgg tggcgtggct accgacccgg attaccgcaa gcacgggtac gcgggaacca
     1141 ttctggccca ttgcctcgaa tacatggacc gcgaactgga ttgcgacctg tccattctct
     1201 ggaccggcat ccctgactat taccgacggt ggggatggga acatgcgggg cagaactgga
     1261 cgttcttcat cgatcgcacc accatcacct atctcccggt cgccccgacc ggcgaagtgc
     1321 ttaccgatac actcgatccc cgcgtcattc aggcagtgga gtcgcttcac gccgcccgca
     1381 gggcgggagt cgtgcgcgac cgcgagctca ccgaggtttt gctcaacatc cgcacgcgcg
     1441 tccggtgtgc cgttctcatg gacggggaaa caccggtggc gtacatcgtg tactcacccg
     1501 gtgaacagat cgacgtgaag gattgtggtg gggatggttc ggctgttctg gggcttctgc
     1561 gcgtcgtgtt cgcccagtgt ggcgcccgga cggcgcagat tcacactccg gcacaggacc
     1621 aatccgtcgc gccacttttt atcgagcgcg ggttcgccgt ccgcgccaat tactccggca
     1681 tgctggctgt cctgaagccg cagcgcatcc tggaggcgta cggaatccgg gatgtgactg
     1741 tacggcctga aggggacggg tggcagatcc agacctcgac ggcgagtgtg tttttgcgca
     1801 aacccgatct ggtgaagctc ctgttcggac ctgaacggcc gccggaaatc ggccattcca
     1861 aactgccgtt gccgttctac tacggccaac tggaccacat gtaacgttca caagtaatcg
     1921 ggagtgcgtg cgatgagatc gaaataccgc gctgagtgct cgccacgcgg gcgatgggtg
     1981 gttgcacacc ggggtttctc gggcaggtac ccggaaaaca ccatggcggc attcagagcg
     2041 gcggtcgcgc tcggagtgga cgttatcgaa attgacgtcc atctctctcg cgatggtcac
     2101 gtggtcatca tgcatgacgc gaaactggac cgcacgacgg acgggaaggg cccgattgcg
     2161 gaacacacgt tggcggagat caaacgggtt gatgccggct ccaaaaaagg cgcacagttc
     2221 gcagg
//
LOCUS       DAOTNG010000424         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38307_length_2216_cov_6.577048, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000424 DAOTNG010000000
VERSION     DAOTNG010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38307_length_2216_cov_6.577048"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>2216
                     /gene="dnaE"
                     /locus_tag="P5179_14025"
     CDS             58..>2216
                     /gene="dnaE"
                     /locus_tag="P5179_14025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRS96382.1"
                     /translation="MDQADRMSMMDGDYSLLSTEEMSSFFDGMPETITNTLRVAEMCN
                     VEIELGKARLPKFPTPKNLSPSEYLRELCEEGLRKRYPLEKAQDGQWRLREGHAEAEL
                     PVPLPEILERLDYELGVIGGMGFDSYFLIVWDYVQYAKKNGIVVGPGRGSAAGALVTY
                     VLGITELDPLRYDLLFERFLNPDRISMPDADVDFDDEHRDQVVEYVRQKYGRDCVANV
                     ITFGTMQAKGAIKDVGRAMGMTFDETDRIAKLVPNRVGVELSECLESEPEFRKAYEEN
                     PRNKKLVDLAMQLEGVTRHVSVHACAVVISDIPLENFTPLQYAPRSEDVIITQYEAHA
                     LESLGLLKMDFLGLRNLTVAKRATELIKRNHGVDVDLYNLPFDDPATYELLSRGETTA
                     VFQLESSGMKRYLRELRPNRFEDIIAMVALYRPGPMQFIEEYCARKHGRAPITYDHEL
                     MVPALESTYGITVYQEQVMQMSRELGGFTRGEADTLRKAMGKKIGSLMDQMKGKFVSG
                     ARERGIGEELATKIWGDWEKFASYAFNKSHAACYAFVAFQTAYLKTHYPYEFMAAALS
                     SEIGDSKRIIVLSDECRRMGVPLLPPNVNKSELQFTVSKEGIRFGLLAVKNVGIHAIE
                     AITHAREQEGPFSSLFDFCRRVDNKAINKRMIESLIKSGAMDDLGERAQMLAGLDAAI
                     NWAQNEQENRSRGQTSLFDAGSSDVAHGPAIEEATPTL"
BASE COUNT          510 a          604 c          678 g          424 t
ORIGIN      
        1 tgtacgactg atgccgaagc gcaggatgcg ctcctttgcg ttcagacctc ggctacattg
       61 gaccaggctg accgcatgtc aatgatggac ggtgattaca gcctcctttc aaccgaggag
      121 atgagcagct ttttcgacgg gatgccggag acgattacga atactcttcg agtggcggaa
      181 atgtgcaacg tggagatcga actggggaaa gcacgcctgc ccaagttccc gacgccgaag
      241 aacctgtcgc cctcagagta cttacgggag ctttgcgaag agggtctgag gaagcggtat
      301 ccgcttgaaa aggctcagga cgggcaatgg cgccttcgtg aggggcacgc tgaggcggaa
      361 ttgccggttc cactgcccga aatcctggag cgccttgatt acgaactggg cgtgatcggc
      421 gggatggggt tcgattccta tttcctcatc gtatgggatt acgtccagta tgcgaaaaag
      481 aacggcatcg ttgtgggccc ggggaggggt tccgcggcag gcgcacttgt tacgtacgtg
      541 ctgggaatca cagaactgga ccctctccga tatgatctcc tgttcgagcg cttcctgaat
      601 ccagaccgca tctccatgcc ggacgcagac gtggattttg atgacgaaca tcgtgatcag
      661 gttgtggaat acgttcgcca gaaatacggg cgcgattgcg tcgccaatgt gatcaccttc
      721 gggacgatgc aggcgaaggg cgcgatcaag gacgtcgggc gcgcgatggg catgacgttc
      781 gacgagacgg accggatcgc caagctcgtt ccaaacagag ttggcgtgga gctttccgaa
      841 tgccttgaat cggagccgga gttccgcaag gcgtatgaag agaacccgcg aaacaagaaa
      901 ctcgtcgacc tggcgatgca gcttgagggg gtaacccgcc acgtatcagt tcacgcgtgt
      961 gcggtggtga tttcggacat ccccctcgag aacttcaccc ccctgcagta cgctccacga
     1021 agcgaagacg tgatcatcac ccagtacgaa gcccacgcgc tcgaatcact cggcctcctg
     1081 aaaatggact tcctcgggct tcggaacctc acggtggcga agcgggcaac cgaattgatc
     1141 aagaggaacc acggggttga cgtggacttg tacaatctgc ccttcgacga ccccgccacg
     1201 tacgaattac tctcgcgcgg cgaaacgacc gcggtctttc agctcgaatc ctcgggaatg
     1261 aagcggtacc tcagggaact ccgcccgaac cgatttgaag acatcatcgc gatggtggct
     1321 ctgtaccgcc cgggaccgat gcagttcatc gaggagtact gcgcaaggaa gcatggccgg
     1381 gccccgatca cctatgacca cgaactgatg gtgccggcgc tcgaaagcac gtacgggatc
     1441 actgtgtatc aggaacaggt gatgcagatg tcgcgcgaac tcggtgggtt cacgcgcgga
     1501 gaagcggaca cgctgcggaa ggccatgggc aagaagatcg gttcgctcat ggaccagatg
     1561 aaagggaaat tcgtttcggg agcccgtgag cgcggaatcg gcgaagaact cgccacgaaa
     1621 atctggggcg actgggagaa attcgcctcc tacgcattca ataagagcca tgccgcctgt
     1681 tatgcatttg tggcgtttca gactgcgtac ctgaagacgc actatcctta cgagttcatg
     1741 gcggctgcgc tttcgtcgga aataggggat tcgaagcgga tcatcgtgct cagcgacgaa
     1801 tgccgccgga tgggcgttcc gcttctgcct ccgaacgtga acaagagcga actgcaattc
     1861 accgtcagca aggaaggaat ccgtttcgga cttctcgccg tcaagaacgt gggtatccac
     1921 gcaatcgagg cgatcaccca cgcacgtgag caagaaggcc cgttctcctc gttattcgat
     1981 ttctgccgtc gggtggacaa caaggcgatc aacaagcgca tgatcgagag cctcataaag
     2041 tcgggcgcaa tggatgacct gggagaacgg gcacagatgc tcgcagggct tgatgcggcg
     2101 atcaattggg cacagaacga gcaggaaaac cgatcccggg gacagacatc tctctttgac
     2161 gccgggtcct ctgacgtcgc gcacgggcct gcgatcgagg aggcaacgcc aactct
//
LOCUS       DAOTNG010000425         2215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38331_length_2215_cov_3.207870, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000425 DAOTNG010000000
VERSION     DAOTNG010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38331_length_2215_cov_3.207870"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5179_14030"
     CDS             complement(<1..328)
                     /locus_tag="P5179_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96383.1"
                     /translation="MHFFAEGTPTDMAPFAERMAWTSGFDSGLQWCEARDIERVVVHW
                     RDDVPAATDVRLEYWKGQWPEKRVPKGQVTGGGFPGWMAEDDWFNGRWQVADTVLTAA
                     DKQWIFT"
     gene            complement(356..829)
                     /locus_tag="P5179_14035"
     CDS             complement(356..829)
                     /locus_tag="P5179_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRS96384.1"
                     /translation="MDTVWGLLSSLGEPAARLTLAVLASSVIGWEREIGGHPAGLRTN
                     IMVALGAAIFTLLSQDIVAKGTGDPTRVIAGVAQGIGFLGAGTIFLDANRIKGLTSAA
                     TIWVMGGLGIAWGAGSYPTAILGTLAAFVVLRTIKRLELVTAEKLRNRVDRRDTL"
     gene            complement(938..1846)
                     /gene="ribF"
                     /locus_tag="P5179_14040"
     CDS             complement(938..1846)
                     /gene="ribF"
                     /locus_tag="P5179_14040"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRS96385.1"
                     /translation="MASTAITVGSYDGVHSGHRAMIAQLCASARERDLQSILVTFEPH
                     HRIYFHRETKPFLLTTTSEKRVLLQETCLDGVIVLPFSAELAALSARDFIKGILVEKL
                     GARLLLVGHDQAFGKDQCTGRDAITACAAPLGVEVLSPAPVSSDGRVVSSTRIRGLIR
                     TGSIAPANHLLGAPYLVSGRVVHGRERGRSLGYPTANLSIEDPFKLIPGEGIYAVFAS
                     CGGSKFAGMGYIGTRSTFGETDPVVEIALLDTNVDLYGQELQVRFVERVRADHRFPNA
                     DALLAQIRCDEQAIRSILAAHRDSVA"
     gene            1924..>2215
                     /locus_tag="P5179_14045"
     CDS             1924..>2215
                     /locus_tag="P5179_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96386.1"
                     /translation="MIGLAGCAPDDGRRYLIIQTALRFGGTAAREIRCVPDGLGQETR
                     SASLRRSFAKNRASVVVGGTTEVGRMSASRNSERGKTIVVIGFPARDTKHMPD"
BASE COUNT          474 a          694 c          628 g          419 t
ORIGIN      
        1 acgtgaaaat ccattgcttg tcggcagcgg tgagaaccgt atcggcaacc tgccaccggc
       61 cgttgaacca gtcgtcctcg gccatccatc cggggaatcc gcctccggtc acctgcccct
      121 tcggcacgcg tttctccggc cattggccct tccagtactc cagtcggacg tccgttgcgg
      181 cgggaacatc atcccgccag tgcacgacaa cgcgttcgat gtcccgcgct tcgcaccatt
      241 gaagcccgct gtcgaatcct gacgtccacg ccatccgttc ggcgaacggc gccatgtcgg
      301 tcggggtacc ctcggcgaaa aagtgcacgg gattctcctc cttgcgcgat atctgctaca
      361 gtgtgtccct tcggtccacc cggttcctaa gcttttccgc ggttaccagt tccagccgtt
      421 tgatggttct cagcactacg aacgcggcca gtgttccgag aatcgcggta ggataagacc
      481 ctgctcccca tgcgatgccg agtccaccca tcacccagat ggtggctgct gaggtgagac
      541 ccttgattcg gttcgcatcg agaaaaatcg ttcccgcacc aaggaaaccg attccctgcg
      601 ctactccggc gatcactcgt gtgggatcac ccgtcccttt cgcgacaata tcctgcgata
      661 ggagggtgaa gatggctgcg ccaagggcaa ccatgatgtt cgttctcaat cccgccggat
      721 gcccgccgat ttcgcgttcc catcctatga cactgctcgc gagcaccgcg agtgtaagtc
      781 gcgccgcggg ctcacccaac gaggataaca aaccccagac agtatccacg acgtcaaacc
      841 ttccctggat tcaacgtttg actcctcaaa aagtcaggcg gaagtttcca aaatacgagt
      901 atggacagca gcgccagtcc acagcgccca ttgaggttca cgctaccgag tcgcggtgtg
      961 ccgcgagaat gctccggatc gcttgctcgt cgcacctgat ctgagccaga agtgcatccg
     1021 cgttcgggaa acggtgatcc gcccggacgc gttccacgaa acgaacctgc agctcctgcc
     1081 cgtaaagatc aacattcgtg tccagaagcg ctatctctac gaccggatcc gtttccccga
     1141 acgtcgaccg cgtacctatg taccccattc ctgcgaattt cgagccgccg caggacgcga
     1201 aaacggcgta gatcccctcg ccgggaatca acttgaatgg atcctcgatc gaaagattgg
     1261 ccgtgggata gcccagggaa cggccgcgtt cccgtccgtg gaccacgcgc ccggagacca
     1321 gatacggcgc gccgagaagg tggttcgcgg gagcgatgct cccggtgcgt atcaagcctc
     1381 tgattcgagt gctggatacc acgcgcccat ctgacgaaac gggagcaggc gaaagaacct
     1441 ccacgccaag aggagctgcg catgccgtga ttgcgtcccg accggtgcac tggtctttcc
     1501 cgaaggcctg gtcatgcccc acgagcaaca gccgtgcgcc aagtttctcc acaaggatgc
     1561 ctttgatgaa atctcgcgcc gaaagagccg caagctcggc cgaaaagggg aggacaatca
     1621 ctccgtccag gcacgtttcc tgcaaaagga cgcgcttttc ggaggttgtc gtcagaagaa
     1681 aaggcttcgt ttcccggtgg aagtaaatcc ggtggtgcgg ttcgaacgtt accagaatcg
     1741 attgaaggtc gcgctcgcgg gcagatgcgc acaactgcgc gatcatagcc cgatgaccag
     1801 agtgtacacc gtcgtaagac ccgacggtga tcgccgtgct ggccatatgg ggaagatcca
     1861 cggaggactg aatgcaagaa aaacgcatga cacaaaaata ctccaagcag ccgcggcggg
     1921 acaatgatcg ggctcgccgg ctgcgcgcca gacgacggtc ggcgttacct tatcattcag
     1981 accgctctcc gctttggcgg aaccgcggcg cgtgaaatcc ggtgcgttcc agatggtctg
     2041 gggcaggaga ccaggtctgc cagtctgcgc aggtcatttg ccaaaaaccg cgcgagtgtc
     2101 gtggtgggtg gcacgaccga ggtaggacga atgtctgcat cccgcaacag cgagcgcggc
     2161 aaaacaatcg ttgtgatcgg ttttcctgcg cgcgacacga aacacatgcc ggacg
//
LOCUS       DAOTNG010000426         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38356_length_2214_cov_3.624363, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000426 DAOTNG010000000
VERSION     DAOTNG010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38356_length_2214_cov_3.624363"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="P5179_14050"
     CDS             <1..463
                     /locus_tag="P5179_14050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS96387.1"
                     /translation="LRRCRQSEVVAVRRLIHETIDASYTGVYPPRALQSFREFHSDEN
                     IAERVRTGTVLVLKREGEIVATGSLVGNEICGVFVRASCQRCGYGRVVMVELEKRAKV
                     NGCARGELSVSLPSRAFYESLGYEITEECSMDVGEGQRLDYWRGRKLLSKG"
     gene            complement(508..1128)
                     /locus_tag="P5179_14055"
     CDS             complement(508..1128)
                     /locus_tag="P5179_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96388.1"
                     /translation="MELENGKRELRSVGRDRVTPIRRCLRLPGYDYSQAGAYFVTLCA
                     HHRECLFGEVLDGQMRMNAAGEMVARWLSEVQYKFPNAKIDAHIVMPNHVHAIVVLRG
                     AVGADLRVRPPEETVTNADGDTTGAHKGAPLPRIVQWIKTMATNGYMHGVESQGWPPF
                     PGRLWQRSYYDHIIRDENDLRRAREYIEMNPARWAEDEEYTGVPCV"
     gene            complement(1136..>2214)
                     /locus_tag="P5179_14060"
     CDS             complement(1136..>2214)
                     /locus_tag="P5179_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS96389.1"
                     /translation="TASHGSGFMLWQSGAYPFGVRQSPWRDGKGDLVKDFVEACRRNG
                     IQPGLYSHLRCNGYWRVDHPGLVNEGKGGDPKRQAAYAKARLTMCRELWGNYGPLAEI
                     WFDGAVPDPGLVGYNVLPYLRSMQPEAMIFQGPTATIRWIGNEKGVANYPCWATVPSV
                     AEAATWESRGMGDRLCTGDPDGATWLPGECDTPIRNHEWMWKPAQEHLLKSVDELMEV
                     YEHSVGRNCNLLINANPDPDGLVPDADMQRYREFGAALRRRYGTPLAETSGRGNAVEL
                     PLPRPAVVDRAILMEEITQGERIREYVLEGRTDAGWAPLARGQSIGHKRIERFPATSV
                     SAVRLRVEKAIAEPWVRRLAVFEE"
BASE COUNT          358 a          698 c          730 g          428 t
ORIGIN      
        1 cctccgcaga tgccggcagt cggaagtggt tgctgtccgg cggttgattc acgagactat
       61 cgatgcgtcg tacacgggtg tctacccccc tcgcgcgttg cagtcgttca gggagtttca
      121 ctcggacgag aacatcgcgg aacgggtgag aaccggaacg gtgcttgtcc ttaagcggga
      181 gggggagatt gtcgccacgg ggagccttgt cggcaacgag atatgcggcg tgttcgtgcg
      241 cgcatcctgc cagcgatgcg gctacggccg ggtggtgatg gtggaattgg agaaacgggc
      301 gaaggtcaac ggctgcgcga ggggggaact tagcgtgtcc ctgccgtcac gggcgttcta
      361 cgagagcctt ggctacgaga tcaccgagga atgctcgatg gacgtggggg agggacagcg
      421 cctagattac tggcggggga ggaagctgct gagcaaggga taaccggccg gtcgtcgccc
      481 gccgccgacg cgtacctcgc ctctcgatca gacgcacggg actccggtgt actcctcgtc
      541 ttccgcccac cgtgcggggt tcatttcgat gtattcgcgg gcacgtcgga ggtcgttttc
      601 atctcggatg atgtggtcgt aataagagcg ttgccagagc cgtcctggga atggtggcca
      661 tccctgggat tcgacaccat gcatgtatcc gttcgtggcc atcgttttga tccattggac
      721 aattcgcggt aggggcgcac ctttgtgtgc gcccgtggta tcgccgtcgg catttgtgac
      781 cgtttcttcg ggcgggcgga cacgcaggtc cgcccctacg gcaccccgca aaaccacgat
      841 ggcgtggaca tgattcggca tcacgatgtg tgcgtcgatc ttcgcgttgg ggaatttgta
      901 ctgcacttct gacaaccaac gggccaccat ctcgcccgcg gcgttcatcc gcatctgccc
      961 gtccaacacc tcgccgaaaa ggcattcacg gtggtgggcg cacaacgtga cgaagtacgc
     1021 tccggcctgt gagtaatcgt aacctggcaa ccggagacat cggcggatcg gagttacccg
     1081 gtcgcggccc acgctgcgaa gctcgcgttt cccgttttcc aactccatcg tgtgttcact
     1141 cctcaaacac cgccagccgc ctcacccacg gctcggcgat tgccttctcc acgcgcaggc
     1201 gaacggcgga taccgatgtc gcggggaacc gctcaatgcg cttgtgtccg atgctctgcc
     1261 cccgcgcaag cggcgcccac ccggcgtccg ttctgccctc gaggacgtat tcgcggatgc
     1321 gctcgccctg cgtgatctcc tccatcagga tggcgcggtc caccacggca gggcggggaa
     1381 gcggcagctc gacggcattg ccccggcctg aggtctccgc cagcggggtg ccgtaccgcc
     1441 ggcgcagcgc cgcgccgaat tcgcggtacc gctgcatatc ggcgtccggg acgaggccat
     1501 cgggatccgg gttcgcgttg atgagcaggt tgcagttgcg gccgacggag tgctcgtaca
     1561 cctccatcag ctcgtcaacg gatttgagga ggtgctcctg cgccggtttc cacatccatt
     1621 cgtggttgcg gatgggggtg tcacattcgc cgggcagcca tgtggcgccg tctgggtcgc
     1681 cggtgcagag acggtcaccc atgccccggc tttcccacgt tgccgcttcg gcgacgctgg
     1741 gcacggtggc ccagcaggga tagttcgcca cgcccttctc gttcccgatc cagcggatgg
     1801 tggctgtggg accctggaag atcatggctt cgggctgcat ggagcgcagg tacggcagga
     1861 cgttgtaccc caccagtccg ggatccggca cggcgccatc gaaccagatt tccgcgagcg
     1921 ggccgtagtt gccccagagt tcccggcaca tggtgagccg ggccttcgcg tacgccgcct
     1981 gccgcttcgg gtcgccgccc ttgccctcgt tcacgaggcc gggatgatcc acccgccagt
     2041 agccgttgca gcgaaggtgc gagtagagcc ccggctggat tccgttccgg cggcaggctt
     2101 cgacgaagtc cttcaccaga tcgcctttgc cgtcccgcca cggggattgt cggacaccga
     2161 aggggtacgc gccactctgc cacagcatga acccggagcc gtgggaagcc gtta
//
LOCUS       DAOTNG010000427         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38631_length_2202_cov_4.980438, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000427 DAOTNG010000000
VERSION     DAOTNG010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38631_length_2202_cov_4.980438"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /gene="pabB"
                     /locus_tag="P5179_14065"
     CDS             complement(<1..1338)
                     /gene="pabB"
                     /locus_tag="P5179_14065"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRS96390.1"
                     /translation="MTENRTGTGHAPRVILRDARHGQWLKFIDPQRTLVATTLQEVLQ
                     VLETIDRAAAKDALYCAGFVSYEATPAFDAGLAAHTPGRFPLAWFCLFRNVEPVDIPA
                     PPANALSRAFRWRPSVSRTEYYEAIHRVHALIESGDTYQVNYTYRLVSEFTSDPWEFF
                     LRLVAAQNPPYGGYIDAGEWAICSASPELFFTRHGSELESRPMKGTAARGRTLEEDLL
                     MSERLQASLKERAENVMIVDMVRNDFGRVARTGSVAAKRLFEIERYPTIWQMTSTVTA
                     RTEQRLQNVFRATFPPASITGAPKIRTTQIIADLETTRRNIYTGTIGFVTPGGDAQFN
                     VAIRTALIDRTTGKAEYGAGSGIVWDSDAESEWEECETKAKVLSAEVPEFQLLETMRW
                     EPRSGYYLKTHHLRRLTESARYFGFKVDMREVRRELRSFARTLPRKAQRIRLLV"
     gene            complement(1331..1828)
                     /gene="rnhA"
                     /locus_tag="P5179_14070"
     CDS             complement(1331..1828)
                     /gene="rnhA"
                     /locus_tag="P5179_14070"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRS96391.1"
                     /translation="MKEIRIHTDGACSGNPGPGGWAAILTYGTHRKSLFGAEPETTNN
                     RMELRAAIEALRALKEPCNVVVVTDSRYLRDGITRWLPTWKKNGWKTSAKDPVKNRDL
                     WEALDCLTGVHSVRWEWVEGHSGDPDNEACDVLAVTAMKKLLKRAPAARTRPRPTAGA
                     THRDD"
     gene            1914..>2202
                     /locus_tag="P5179_14075"
     CDS             1914..>2202
                     /locus_tag="P5179_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96392.1"
                     /translation="MATGRDGPTMIEDLNPAFDMFLFPAKVSARARRYFSLFDRALAN
                     LLAHFDVEAGLISHEVPEGSPGVSEEVADLDRFERKKYISPLNKWAYGVGRQ"
BASE COUNT          417 a          652 c          637 g          496 t
ORIGIN      
        1 caccagcagg cggattcgtt gtgcctttcg aggaagcgtt ctcgcgaagg atcggagttc
       61 gcgccgaact tcacgcatgt caaccttgaa gccaaagtag cgtgcacttt ctgtgaggcg
      121 ccgcaggtga tgtgtcttca ggtaataccc tgaccgaggt tcccagcgca tcgtttcaag
      181 aagctggaat tcgggtactt ccgcggaaag caccttcgcc tttgtttcgc actcctccca
      241 ttcggattcc gcatctgagt cccagacgat tccgctgcct gcgccgtatt ctgccttgcc
      301 cgtcgttctg tcaatcagcg cggtgcggat ggcaacgttg aactgcgcgt ccccgccggg
      361 agtcacaaac cctattgtcc cggtgtatat gttgcgtcgt gtcgtttcca ggtccgcgat
      421 gatctgtgtg gtccggattt ttggcgcgcc ggtgatggaa gccggtggaa atgttgcccg
      481 gaacacgttc tgcaggcgct gttccgttct cgcggtgacc gtcgaggtca tctgccagat
      541 ggtcggatac cgctcgattt caaaaagacg tttggccgcc acagacccgg ttcgggctac
      601 tctaccgaaa tcgtttcgaa ccatgtcgac gatcattacg ttttctgccc gttccttcaa
      661 cgaggcctga agtcgttcgg acatgaggag gtcttcctcg agcgtcctgc cgcgggctgc
      721 agttcctttc atcgggcgtg attcaagttc gctgccatgg cgggtgaaga ataactccgg
      781 agaggcgctg catatcgccc attcccccgc gtcaatatac ccaccgtaag gaggattctg
      841 ggctgcgacc agtctcagga agaactccca cgggtcagag gtgaactccg acacgagacg
      901 gtaggtgtag ttcacctggt aggtgtcacc ggattcgata agtgcgtgta cccggtggat
      961 ggcctcgtaa tactccgtcc gggataccga aggccgccaa cggaacgcgc gcgacaacgc
     1021 gttggcgggg ggcgccggga tatcaaccgg ttcgacgttt ctgaacaggc agaaccaggc
     1081 gagcggaaac cttccaggag tatgcgccgc aagccccgcg tcaaacgcgg gcgtcgcctc
     1141 gtaggaaaca aaccccgcgc agtagagggc atcctttgca gcagcccggt caatcgtttc
     1201 gagaacctgc agcacctcct gcagcgtggt ggcaacgaga gtcctctgcg ggtcaatgaa
     1261 tttcagccac tgcccgtgtc gagcgtcccg cagaattacc ctgggggcgt gtcctgttcc
     1321 tgtccgattc tcagtcatcg cggtgtgtcg ctcccgcggt tggtcgcggt cgagtacggg
     1381 cggccggtgc acgcttgagc agtttcttca tcgcggttac cgcaagcacg tcgcaggctt
     1441 cgttgtccgg atcgccagaa tgcccttcca cccattccca tctgaccgag tgaaccccgg
     1501 tgaggcaatc gagtgcctcc cacaggtccc ggttcttcac cggatccttt gccgaggtct
     1561 tccagccatt ctttttccag gtaggaagcc accttgtgat gccgtctcgg agataccgcg
     1621 aatccgtcac gacaaccaca ttgcagggct cctttaacgc gcgaagtgcc tcaatcgctg
     1681 ccctgagttc catgcgattg ttggtggtct cgggttctgc accgaacaga gacttgcggt
     1741 gcgttccgta cgtcagaatc gccgcccacc cacccggacc agggttgccg gaacatgcac
     1801 cgtccgtatg tatgcggatt tctttcatcg tacctcgaca gttgctgcgt gacgccttgg
     1861 gaatctaaaa ttcgagaacc tttcgcacca gacaaagctt ccgacgtgtg gtgatggcaa
     1921 ccggacggga cgggccaacc atgatcgaag acctcaaccc tgcgttcgat atgtttcttt
     1981 ttcccgccaa agtgtccgcc cgcgctcgac ggtacttcag ccttttcgat cgggcgttgg
     2041 cgaatttgct ggcacatttt gacgtggagg cgggattgat cagccacgag gttcccgaag
     2101 gttcgcccgg tgtctccgaa gaagttgccg acctggacag gttcgagcga aaaaagtaca
     2161 tctcgccgtt gaacaagtgg gcgtacggcg tcggaaggca ga
//
LOCUS       DAOTNG010000428         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_38684_length_2200_cov_3.547319, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000428 DAOTNG010000000
VERSION     DAOTNG010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38684_length_2200_cov_3.547319"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="P5179_14080"
     CDS             <1..2054
                     /locus_tag="P5179_14080"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS96393.1"
                     /translation="GIPVGWTYSGPAARQDETWPDGGAHGGTRSIRFAARTDRQAWTS
                     ERVPVQGSVPYRLEWWTSLAGSTPWHWTTHTGFIGVRVTFFDAAGDRCGITERRIRSI
                     GTDGWVRCWLDVNPPPEASSCTVSLVCEAEIETDGTIAFDDVALVPAGMIQTPAGWGR
                     LRGVARGGEDQAPGFARVLITAADGRHYAPENRCQFLDGSFHATGEPFELALPAGRAA
                     VLVTRGFEYSIWRDTVDIRAGQRTEVAPRLAHRWPLSAEGWYSGDAHIHLFFHRHSLH
                     PQMVPADVFALARAEGLGWLSFKGEEKEVREFIARSAPLCVPGFTGEIGIEAVSDFHG
                     HVYTLNARTIPADGFPMKLVPWPMNLDLGAALDSAGGALVYAHPSGDLAAGGLLEGIA
                     DPARLLLAREWPVDLALGQNPAFDILAPDGPNATAVKLPVYYRMLNAGFRSAVTASTD
                     AYVDQGRMHPGAARTYAYTPSLTWSEIADAHRKGATFATNGPLVWLSVNGKRPGEEAR
                     VARNGSAEVQFRAASNRGLTRAEVIVNGRVAYTAFPGADGEIRGSFVLPVSESCWVAL
                     RVFGPRGEGLADELIPDEWKAEGIGQFAHTSPVYVMVDGRAMRPDPEAARFFAEWIAA
                     YRNAVLNRADLFENENGTWGEDVKARILERLDRAEAVFSRRAQEGRSGAPARRRANGA
                     R"
BASE COUNT          342 a          640 c          850 g          368 t
ORIGIN      
        1 acggtatccc ggtcgggtgg acgtattccg ggcccgcggc gcggcaggac gagacatggc
       61 cggatggcgg cgcgcacggg ggaacgcgca gcatccgttt tgcggcgcga acggatcggc
      121 aggcatggac aagcgagcgg gtaccggttc aggggagcgt gccgtaccgg ctggagtggt
      181 ggacatcgct cgcgggcagc acgccatggc actggaccac gcacaccggc ttcatcgggg
      241 tgcgcgtcac gttcttcgac gcggcgggcg accggtgcgg catcaccgag cggcgcatcc
      301 gctccatcgg aacagatggc tgggttcggt gctggctgga tgtcaacccg ccgccggagg
      361 cttcgtcgtg cacggtgtcc ctcgtgtgtg aggccgaaat cgagacggac ggcaccatcg
      421 cgtttgacga tgtggcgctg gtgccggcgg ggatgatcca gacgcccgcc gggtggggcc
      481 gtctgcgagg agtggcgcgg gggggagagg atcaggcgcc ggggtttgcg cgggtgttga
      541 tcaccgccgc ggatgggcgc cactatgccc cggagaaccg ctgccagttc ctcgacgggt
      601 cgttccacgc cacgggggag ccgtttgagc ttgcgctccc tgcgggtcgt gcggcggtgt
      661 tggtcacgcg cgggtttgag tattcgatct ggcgggatac ggtggacatc cgggcagggc
      721 agaggaccga ggttgctccc cggctggcgc accggtggcc gctttccgcc gagggatggt
      781 acagcgggga cgcgcacatt cacctgttct tccaccggca ttcgctccac ccgcagatgg
      841 ttccggcaga cgtgttcgcc ctcgcgcggg cggaagggct cggctggctt tcgttcaagg
      901 gggaagaaaa ggaggtgcgg gagttcattg cgcgttctgc tccgctgtgc gtaccgggat
      961 tcaccggaga gatcggcatt gaggcggtga gcgatttcca cgggcacgtg tacaccctca
     1021 acgcgcggac gataccggcg gatggcttcc cgatgaagct cgtgccgtgg ccgatgaacc
     1081 tcgatctggg cgctgcgctg gattccgccg gcggcgcgct cgtgtatgcg cacccgtcgg
     1141 gcgatctggc ggcgggcggg ttgctggagg gcattgccga cccggcgcgg cttctgctgg
     1201 cacgggaatg gccggtggat ctggcgctgg ggcagaaccc cgcgtttgac atccttgcgc
     1261 ctgacggacc gaatgcgacg gcggtgaaac taccggtgta ttaccgcatg ctgaatgccg
     1321 gattccgttc cgccgtgacg gcttccaccg acgcgtacgt tgaccaggga cgtatgcacc
     1381 cgggcgcggc gcggacgtac gcatacactc cctcgctgac gtggagcgag attgctgatg
     1441 cgcaccggaa gggcgcgaca ttcgctacga acggtccgct ggtgtggctc tccgtgaacg
     1501 gaaaacggcc gggcgaggag gcgcgggtgg cccggaacgg gagcgctgag gtgcaattcc
     1561 gggcagcatc gaaccggggg cttacgaggg cggaggtcat cgtgaacggg cgcgtcgcct
     1621 acactgcttt ccccggtgcg gacggggaga tacgcggctc gttcgtgctc cccgtttccg
     1681 aaagctgctg ggtagccctg cgggtgttcg ggccgagggg agaggggctg gcggatgagc
     1741 tgattcccga cgagtggaag gcagagggga tcgggcagtt tgcccacacg agccccgtgt
     1801 acgtcatggt ggacgggcgg gcgatgcgcc cggatccgga agcggcgcgg ttcttcgcgg
     1861 agtggattgc ggcgtaccgg aacgcggtcc tgaatcgcgc cgacctattc gagaacgaaa
     1921 acggcacgtg gggggaggac gtgaaagcgc gcatcctcga acggctcgac agggcggagg
     1981 cggtgttttc ccggagggcg caggagggtc gcagcggtgc acctgcgcgc cggcgggcaa
     2041 atggcgctcg ataaagtgat gcctttgccg ggcttcgaag gccgataagt cgagggtcag
     2101 ggactggaaa gggcccaacg aagcgggccc ctccatgcgg cgtgaagtgg agagcggcgc
     2161 gtcgcctgcg cttctacagc tccagccgcg taccgtcgtt
//
LOCUS       DAOTNG010000429         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39369_length_2172_cov_4.093529, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000429 DAOTNG010000000
VERSION     DAOTNG010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39369_length_2172_cov_4.093529"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="P5179_14085"
     CDS             complement(<1..671)
                     /locus_tag="P5179_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96394.1"
                     /translation="MPETVNVIVPVKRMSPKGEHCFFGYYDIPAADRAGRHLCHRVQF
                     RDRLPRRGDVAELGWLALPGNADAEPDGIFHPIAETEAWNFQQSSMAQWLASEPDTCL
                     FNAFEHEAYGSRIHDIRTGRSRLLPLPVANVSRDGTKALCINMSRLFDFRPGYGYEEL
                     PDPFADVAQPRDDGVRLMDIRTGSNRLILSLADIVEFLEQSGERIGGKKVLINHITFN
                     PSATR"
     gene            complement(686..1741)
                     /locus_tag="P5179_14090"
     CDS             complement(686..1741)
                     /locus_tag="P5179_14090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS96395.1"
                     /translation="MIQKFTVSRDHTIYEAWPDVALTPSGRLVCVFSECTHHGDRSYT
                     RIMLTDSTDRGRTWTPKRPLTEPTRGLPVFWNCARITYLRDGRLVVVVDKLFKPEGSA
                     APEDCRNYLFFSEDEGATWSPPVETPALGIVPDRLLELASGRWILACHFHDKPFGYLV
                     QRLWYSDDRGASWTGPVIVGKKEGLNLCEASILPVRDSLVAFHRENSGQGWDCYKSVS
                     TDNGETWSPVVAFPLPGCHRPVAGVLLDGRILITYRFMQGGKGWVGWWTQNFFAALTD
                     KESALAETRNEAHTRIMPVDFDRSPVSDTGYSGWVQFEDGEIYIVNYIVDDAPKAQIR
                     GYSLHPSDFLFDEPSQP"
BASE COUNT          420 a          675 c          650 g          427 t
ORIGIN      
        1 aaccgtgttg ccgacggatt gaacgtgatg tggttgatca gcaccttctt gccgccgatg
       61 cgctcccccg attgttccag gaactcgacg atatccgcca gtgacagaat cagccgattg
      121 ctccctgttc gaatgtccat caggcggacg ccatcatccc gcggctgtgc aacgtccgcg
      181 aacggatcgg gcagttcttc gtagccgtac cccggccgga aatcgaacag gcgggacatg
      241 ttgatgcaga gcgccttggt gccgtcccgg gagacgttcg ccacgggcaa cggcagcagt
      301 cgactccgtc ccgttcggat gtcgtgaatg cgcgagccat atgcctcgtg ctcaaacgcg
      361 ttgaacaggc aggtatccgg ctctgacgcg agccactgcg ccatggagct ctgctggaag
      421 ttccacgcct cggtctccgc gatgggatgg aatatcccgt caggttctgc gtctgcatta
      481 ccgggcagtg ccagccatcc gagttccgcc acgtcgccgc gccgaggcag gcggtcgcgg
      541 aattgcacgc ggtggcagag gtggcgccct gccctgtccg cggccgggat gtcgtaatac
      601 ccgaaaaaac aatgctcacc tttcggcgac atgcgtttga ccgggacgat cacattcacg
      661 gtttccggca tggtgtcctc actgttcagg gttgtgacgg ttcgtcaaaa aggaaatcgg
      721 acgggtggag cgagtacccg cgaatctgcg ccttcggtgc gtcgtccacg atgtagttca
      781 cgatgtagat ttcgccgtcc tcgaactgca cccagccgga gtagcctgtg tcgctcaccg
      841 gcgagcggtc gaaatcaacg ggcataatgc gtgtgtgcgc ctcgttccgt gtctcggcaa
      901 gcgctgattc tttgtccgtc agcgcggcga aaaagttctg cgtccaccag ccaacccatc
      961 ccttgccacc ctgcatgaac cggtacgtta tcaggatgcg accatcaagg agcacgcctg
     1021 ccacggggcg gtggcaaccc ggcaggggaa atgccactac cggcgaccag gtctccccgt
     1081 tgtccgtcga gacgcttttg taacaatccc acccttgccc tgagttttcg cggtgaaatg
     1141 ccaccagtga atcgcgcacc ggaaggatcg aggcttcgca caggttcagg ccctccttct
     1201 tccccacgat caccggtccc gtccacgagg cgcccctgtc gtccgaatac cagagccgct
     1261 gcacgaggta gccgaaaggc ttgtcgtgga aatggcaggc gagaatccag cgcccgcttg
     1321 ccagctcgag cagcctgtcc gggacgatac caagtgccgg tgtctcgaca ggcggcgacc
     1381 acgtggcccc ctcatcctcg ctgaagaaca ggtagttccg gcaatcctcc ggcgcagcgc
     1441 tgccttcagg cttgaaaagc ttgtccacca ccaccaccag ccggccgtcg cgcaggtacg
     1501 tgattcgggc gcagttccag aacaccggca ggccgcgggt gggctcggtg agcggccgtt
     1561 taggcgtcca cgtgcgccct cggtcggtgg agtcggtcag catgatgcgc gtgtaggagc
     1621 ggtcgccgtg gtgcgtgcac tcggaaaaca cgcataccag cctcccggac ggtgtcagcg
     1681 ctacgtccgg ccacgcctcg taaatcgtgt ggtcgcggct gacggtgaat ttctggatca
     1741 tggtgtctcc ttgtttggat ccggtgccat cgtgaaggaa gctacttccg gaacacattc
     1801 tgccgaaagt cagtccgggt gccgtatcaa gctgaggcaa aacgtcgtct tcgtgtcaag
     1861 ggttttcgcg aaaatagttc aatcggggta cggttgtttg cggggcaaga acaatgacgc
     1921 acacgaagcc gcgaaggacg gaaaaccagc gcacacgagg gccgctccgg caatcccacg
     1981 aatgcggcgt ttcctataca tcctatggga cccaaacagg acctgtggag gggcacgctc
     2041 cgtcgtgccc gtgttctcct gcgcaccaat tcgtcgacgg gcgcatgaaa tctgcctccc
     2101 acactccagc caaggcaaaa cgtcgtcttc gtgtcaagct ttttcgcgaa gaagttgcaa
     2161 atttgcacca gg
//
LOCUS       DAOTNG010000430         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39496_length_2167_cov_3.279356, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000430 DAOTNG010000000
VERSION     DAOTNG010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39496_length_2167_cov_3.279356"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="P5179_14095"
     CDS             complement(<1..713)
                     /locus_tag="P5179_14095"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRS96396.1"
                     /translation="MENLPTPALVVDGAVLRANIERLAAYARGHRLRVRPHTKTHKSV
                     FIASLQVGAGASGLTAAKAGEAEIMATVCEDILVAYPPVDPERCARIATLATHRTVRV
                     ALDSITAAQRMAQAAAAAQSRVGVLVDVDVGMGRTGVQTPEEALRLAEVIDRDPSLHL
                     DGIMYYPGHVWSPPEEQHEALQKVATKLEEVIRFWTKGGLKADIVSGGSTPTAYQSHL
                     VPQTTEIRPGTYVFNDMNTV"
     gene            868..>2167
                     /locus_tag="P5179_14100"
     CDS             868..>2167
                     /locus_tag="P5179_14100"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRS96397.1"
                     /translation="MSCPNSRHDLLYELPSLEWKTGAPLGNGHLGVMAWGDGSPLCLT
                     LDRDGLWDLRATPPPDDADYNWGTIRRLAKSGSWDELAAIMERNHYYHAPTPAKLPVG
                     RMEMWVPGSGAKRGFSARLSLRNATLTAQIGTVRLEVFVAATAPVIAVRLSGGADGIR
                     VRWRSLSDLNAGQARELGLERHRIVSISGASAAVQRLPDGTTSVVAWKQIRTGSGITL
                     LCTIATSRDGPNPEQIAARILNGVAHSLASMRRQHVNWWRRFWSKSEITIPEKRLETL
                     WFYGLYKLASSSRAGHLPANLQGLWVTDGVLPPWVGEYAANMNVQETYWPAYATNHLE
                     LGTPLYDWVEQIAEGGKERTRRFFGWDGLRLESALAADGTPVPSWGTVQFWPGAATWL
                     AHHFWLHWRYSRDLEFLRRRAYPLFRACMDFWARFLEEDED"
BASE COUNT          395 a          641 c          690 g          441 t
ORIGIN      
        1 accgtattca tatcgttgaa gacgtacgtt cccggacgaa tctcggtggt ctgcgggacc
       61 agatgggact ggtacgcggt aggcgtggag cccccagaca cgatgtcagc cttcaatccg
      121 ccctttgtcc agaacctgat gacctcttcc agtttcgtcg cgactttctg cagcgcctca
      181 tgctgttcct caggagggct ccacacgtgc ccggggtagt acatgatccc gtccagatgg
      241 agcgagggat cgcggtcgat cacttcagcg agacggagag cctcttccgg tgtctggaca
      301 cccgtcctgc ccatgccaac gtccacatcc acgagtactc ccactcgtga ttgcgcggcg
      361 gcagcagcct gtgccattcg ctgggccgcg gtgatcgagt ccaacgcaac gcgcacggtt
      421 ctgtgcgtgg ccagtgtggc gattcgggcg catcgctccg ggtccaccgg cgggtacgct
      481 acgaggatat cctcgcagac cgttgccatg atttccgcct cgcccgcttt ggcggcggtc
      541 aagccacttg cgcccgcgcc tacctggagc gatgcgatga aaactgattt gtgtgtcttc
      601 gtgtgagggc ggactcggag gcggtgtcca cgcgcgtaag cagcaagacg ttcgatattc
      661 gcgcgcagaa ccgcgccgtc aaccacgagt gcgggagtag gcaggttttc catgggtatg
      721 aataaccgag cgtgagggcg tgcgagtcaa tccgtatcgg cgcaacggta ttcccgttgg
      781 gcatgggtgg gctttgccgt acgttaagtt aacgtctgac caattcgaga aaacagccca
      841 tcagcctgtc gagatagagc ccttccgatg tcctgtccga attcgcgcca cgacctgctc
      901 tacgaactcc cgtcattgga gtggaagacg ggcgctcctc tggggaatgg ccatctgggt
      961 gtcatggcgt ggggtgatgg gtctcctctg tgtctgactc ttgatcgtga cggtctctgg
     1021 gatctccggg cgacgccgcc acccgacgat gcggactata actggggtac catcaggcgt
     1081 ctcgcgaagt ccggctcatg ggacgagttg gcggcgatca tggaacggaa tcattactac
     1141 cacgcgccca cccctgccaa gcttcccgtc gggaggatgg agatgtgggt gcctggcagc
     1201 ggcgcaaaac gcggtttttc cgcccggtta tcgctcagga atgccacgct caccgcgcag
     1261 atcggcaccg ttcgcctgga agtgttcgtt gcggccaccg cgcctgtcat cgccgtgcgg
     1321 ctcagcgggg gagccgacgg tatccgtgtt cgctggcgat cactcagcga tctgaacgcc
     1381 gggcaagcgc gggagcttgg cctcgagcga cacaggattg tctccatctc gggcgcaagt
     1441 gccgcagtcc aaaggttacc tgacggcaca acctcagtgg ttgcttggaa gcagattcgc
     1501 accggttctg gcattacgtt gttgtgcacg atcgcgacat cgcgtgatgg tccgaaccct
     1561 gaacaaatcg cggcgagaat actcaacggc gttgcccatt cgctcgccag tatgcgcagg
     1621 cagcatgtga actggtggcg gcggttctgg agcaaatcgg agatcaccat tcccgagaaa
     1681 cggctggaaa cgctctggtt ctacggcttg tacaagcttg catcctcctc gcgagcgggg
     1741 catcttccgg cgaacctgca gggcctgtgg gtgaccgacg gcgttctgcc accgtgggtc
     1801 ggtgaatacg ccgcaaacat gaacgtgcag gaaacctact ggccggcata tgcaacgaac
     1861 caccttgagc tgggcacgcc tctttacgac tgggtggaac aaatagcgga gggagggaag
     1921 gagcgcacca ggaggttctt cggctgggac ggccttcgtc tggagagcgc actggcagca
     1981 gacggtacgc cggtgccatc gtgggggacc gtccagttct ggcccggagc ggccacctgg
     2041 ctggcacacc atttctggct ccactggcgt tattcacggg atctggagtt cctccggcgg
     2101 cgcgcgtatc ccctgttccg ggcatgcatg gatttctggg cgcgattcct ggaagaggat
     2161 gaggacg
//
LOCUS       DAOTNG010000431         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39621_length_2161_cov_5.966762, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000431 DAOTNG010000000
VERSION     DAOTNG010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39621_length_2161_cov_5.966762"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="P5179_14105"
     CDS             <1..428
                     /locus_tag="P5179_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96398.1"
                     /translation="GMVAGLLLAVVSVSGLLSALAGKHNGAELFVLGWLGPLAVISAL
                     LIWVAVSLVFRAVKGFEVECDGTVVSVVRFSPHSSKELMRCPITECELFSGGTDLAFR
                     FARPGSGKKEVLDTHYRLSPEEHLAIERLFLIREPSGNA"
     gene            519..1361
                     /locus_tag="P5179_14110"
     CDS             519..1361
                     /locus_tag="P5179_14110"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HRS96399.1"
                     /translation="MQSAIALEGVSFSYGENEVLSAVTFALGSGEFVGIIGPNGSGKS
                     TLLRVMAGILRPSSGVVTMAGRPMQNLSRREIARSVAVVPQETTFVFPFTALEVVLMG
                     RSPHLGRFGIEGEDDLEVARQAMEATDTWFLRDRTMEEVSGGEKQRVIVARALAQKPE
                     ILLLDEPTNALDIRHQVDIYQLLQRLHDESGLTIVLISHDLNLSARACERLILLHRGR
                     IQCDGGPDEVITSALLSEVYETELEVHTDPATGAPIITTPIDRVVRKKSPPFRAGEQQ
                     RKIS"
     gene            1478..1816
                     /locus_tag="P5179_14115"
     CDS             1478..1816
                     /locus_tag="P5179_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96400.1"
                     /translation="MESEVIAEPVAADALAAMRKVEDEAEALVAHARAEAKELLERAR
                     REAEERRSREHALMQREMELRRQELLNGVLRQTEALKADAEAEKASYAAEVRRITPQL
                     VEEIVALVVG"
     gene            1865..>2161
                     /locus_tag="P5179_14120"
     CDS             1865..>2161
                     /locus_tag="P5179_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96401.1"
                     /translation="MASASAFLNARAHALLGMLLPRETLLRLADLPSAEAILADLSAR
                     EYFRASLPAGQLTFPQRLNLELDADYRSTLLVLPQESRPLVRALYRLREVAFLKA"
BASE COUNT          416 a          595 c          667 g          483 t
ORIGIN      
        1 gtggtatggt ggccggtttg ttgttggctg ttgtgagtgt ttccggcctg ctttccgcac
       61 ttgcagggaa gcataacggc gcggaactgt tcgtactggg atggcttggg cccctcgccg
      121 tgatttcggc gttgctgatc tgggttgccg tttcccttgt gttccgcgca gtgaaagggt
      181 ttgaggttga gtgcgacggt acggtcgtat ccgttgtgcg tttttcgccc cacagcagca
      241 aggaattgat gcgctgtccg atcacagaat gcgaactctt cagcggcggc acggaccttg
      301 cctttcggtt tgccaggccg ggaagcggca aaaaggaagt gctggatacc cattaccgac
      361 tcagccccga agagcatctc gcaattgaac gactttttct tattcgcgag ccttccggaa
      421 atgcgtgaaa agcgaactgg atcctcacgc gtgctcaatt gtccgtgcac agcgtctcca
      481 catgtgtctg agcggccgcc tcgatgaacg aatccaaaat gcaatctgcg atcgccctgg
      541 agggtgtctc gttctcatac ggcgagaacg aagtcctttc cgctgtcaca ttcgccctgg
      601 ggtccggcga atttgtggga atcatcggcc cgaatggctc gggtaagtcc acgcttctcc
      661 gcgtcatggc gggtatcctg cgcccttcgt ctggagtcgt gacgatggcg ggacgcccca
      721 tgcagaacct cagccgccgg gaaatcgccc gcagtgtcgc cgttgtgcct caggagacga
      781 cctttgtgtt tccatttacc gcactcgaag tggtgctgat ggggcgctca ccccatcttg
      841 gccgtttcgg gattgagggc gaggacgatc tggaagtggc gcgacaggcc atggaggcta
      901 ccgatacgtg gttccttcga gatcggacga tggaagaagt gtcggggggc gagaaacagc
      961 gtgtcatcgt cgcgcgtgcc ctggctcaaa agccggaaat cctgctcctg gatgagccca
     1021 caaacgctct tgacatccgg catcaggttg acatttacca gctcttgcag cgattgcacg
     1081 atgagtccgg tctcacgatc gtgctgattt cccatgacct gaacctttcg gcgcgagcgt
     1141 gtgagcgcct tatcctcctc caccgcgggc gcatccagtg cgacgggggt cccgacgaag
     1201 tgatcacgag cgcgcttctg agcgaggtct acgaaacgga gctcgaggta cacactgatc
     1261 ctgctaccgg tgctccaatc atcacgactc cgattgaccg ggttgtgagg aaaaaaagtc
     1321 cgccgttccg cgcgggagag cagcaacgaa aaatatcctg aggaggttac gctttttgct
     1381 tgcttcgagt acgtcatgct gctattattg gaaccatgtg gaacatgcaa acgtgaggga
     1441 tttccaacgc ggcgcgcttc tgagcaaggg gtgagatatg gagtccgaag ttattgccga
     1501 accggttgcg gctgatgctc ttgctgcgat gcggaaggta gaggacgaag ccgaagcgtt
     1561 agttgcgcat gcacgggccg aggcaaagga gcttctggag cgtgcgcgtc gcgaagcgga
     1621 agagagacgt tcccgggagc acgctctcat gcagcgcgag atggagctca gacggcagga
     1681 gcttctgaac ggtgttcttc gtcagacgga agcattgaaa gccgacgctg aagctgaaaa
     1741 agcaagctac gccgctgaag tgcgccgcat aactccgcag cttgtcgagg aaatcgtcgc
     1801 tctcgtcgtt gggtaacacc gcgtcagatg ccggtgtgat tccgtccttt tgggggaacg
     1861 gggcatggcc tcagcctcgg cgtttttgaa tgcacgcgcg catgcccttc tcggcatgtt
     1921 gctgccgcgg gaaacccttc tgcgacttgc tgatcttccc agcgcagaag cgattctggc
     1981 agacctttcg gcgcgcgagt actttcgggc ttcgctgccg gcggggcaac tgactttccc
     2041 ccagcgtttg aacctcgaac tcgacgcgga ttaccgatcc accctcctcg tgctgccgca
     2101 ggaaagccgg cctttggtac gggcgctata tcgtttgagg gaagtggctt tcctcaaagc
     2161 c
//
LOCUS       DAOTNG010000432         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39628_length_2161_cov_3.854701, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000432 DAOTNG010000000
VERSION     DAOTNG010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39628_length_2161_cov_3.854701"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5179_14125"
     CDS             <1..248
                     /locus_tag="P5179_14125"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HRS96402.1"
                     /translation="GKWVVSDTNWPDIGLILINTETGLWAPLCKAEASEGHPQWTHPH
                     PMFTPDGRHIVFNSDRTGIGQIYTVEIPESLRQQLSG"
     gene            454..1176
                     /locus_tag="P5179_14130"
     CDS             454..1176
                     /locus_tag="P5179_14130"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRS96403.1"
                     /translation="MLISTARAPEQTSGANPACTGPGTPPDSSATTGIVLQKHVRSLG
                     RFSPSFFQTVDERDSVFSATAKSRAVRGVHGEVLARVSPRFYRRLCVEGSGRLLDGRV
                     VTFSRRHGGEPRFRVTSSQYGLTCRATPLIPYRSVAVDRRQVRLGSVLYIPAVEGLVL
                     PDGSVHDGVFFADDVGGAVRGRKMDFFVGLSSHTENPFTTSQRILHARPVEVFLIPPE
                     LAERYSRLHRVLADRESASAPD"
     gene            1523..2137
                     /locus_tag="P5179_14135"
     CDS             1523..2137
                     /locus_tag="P5179_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96404.1"
                     /translation="MTAASHARNTTIQRILGIARRCCVVGVLGSMCGCNPFQTGEPES
                     PTGALIQYPSPSTTDNVLAILSLSMRAKDSRAFLDRLTADFRFLPDPVQIQGPDFRTF
                     PAEWGTVQEEMALAVLFSNSDSVTVDWSSVFTQHRVEGASVGATYRVNVFSRTSLTAS
                     YTGRVEIVMTQEAGSWAVREWRDYVVSGESNTWGLLRARLLGSG"
BASE COUNT          406 a          649 c          649 g          457 t
ORIGIN      
        1 acggcaaatg ggtggtctcg gacacaaact ggccggacat cgggttgatc ctgattaata
       61 cggaaaccgg cctctgggcg ccgctgtgca aagcggaggc gtcagagggg catcctcagt
      121 ggacacaccc gcacccgatg ttcacgcctg acggacggca catcgtattc aattcggacc
      181 gcacggggat cggccagatt tacaccgtcg agattccgga gagtctgcgc caacagctat
      241 cgggttgaca cgcaggcgac cagcactttt tcggtaccgc cggcctgtct cgcggcgcgc
      301 acggatcggt cacataatgg agatgcgaag ctgacacgcc ttatctccct gcgggagtct
      361 tttcgggggg attttgtccg aagatcgctc aggtccccgc tacgccggct ggacggacgg
      421 ttcgggttct gcgcgtgggt ggtttctgcc ttcgtgctga tttcgaccgc acgggctccc
      481 gaacagacgt cgggggccaa tccggcatgc accggccccg gaactcctcc ggacagttct
      541 gcaaccaccg ggattgttct tcagaaacac gtgcgctccc tggggcgctt cagcccatcc
      601 tttttccaga ccgtagacga acgcgacagc gtgttttcgg caaccgcgaa atctagagcg
      661 gtcaggggag ttcacggtga agtgcttgca cgggtttcac cgaggttcta ccgccggctc
      721 tgcgttgagg gaagcggcag attgctggat ggccgcgttg ttactttttc ccgtcggcat
      781 ggcggagaac cgcgtttccg ggtcaccagt tcgcagtacg gcctgacgtg ccgggccact
      841 ccgttgattc cgtaccgatc cgtcgccgtg gaccggagac aggtcaggct tgggtccgtc
      901 ctttacatac ccgccgttga gggacttgtt ctgcccgatg ggtctgttca cgacggagtg
      961 ttcttcgcag atgacgttgg tggcgcggta cgcggccgaa aaatggactt cttcgtcggg
     1021 ctcagcagcc atacggaaaa cccgttcacc acttcgcaac gcattcttca cgctcgcccg
     1081 gtcgaagttt ttctgattcc tcctgaactg gccgaacgct actcccgact gcaccgcgtt
     1141 ctggccgata gagagtccgc ctcagccccg gattgaggaa acgccgttcc cccttcggcg
     1201 aaacaatagc cgtaacgctc acattccgat cagcccccga caatcctcgc ctgaacgtgg
     1261 ggaggccgac gtcggaataa tgcttttccc aagaatccac cgatttcttc gcgaccggga
     1321 aacgcggttt ggtgcccgga caccctgcgg cggtgggttg aagaggtatt ccccggcata
     1381 accgatgggt tcgggacgct gcgagagggg tttatgactc ccgtcagacg aactatcttc
     1441 cgtagaagaa gcctcaaacc tgacgcgccg cagagtgggc ccgggatttc ggtttcccac
     1501 gttctgaaag tggtcgccgg caatgacagc agcatcccac gcgcgaaaca ccacaatcca
     1561 gcgaattctc ggcattgccc ggcgttgttg cgtcgtcggt gtgctgggat cgatgtgcgg
     1621 atgcaacccg ttccaaacgg gcgaaccgga atccccgacc ggtgctctga ttcagtaccc
     1681 ctcaccctca acaactgaca acgtcctggc aatcctgtca ctttccatgc gcgcaaaaga
     1741 cagtcgcgca ttcctcgacc ggttgaccgc ggatttccgt ttcctgccag atccggttca
     1801 gatacagggt cccgatttca gaacgtttcc cgctgaatgg ggcaccgtcc aggaggaaat
     1861 ggcgttggcg gtgcttttca gtaacagcga cagcgtcacc gtcgattgga gttccgtctt
     1921 cacacagcat cgtgtggaag gggcctcagt gggcgcgacg taccgggtca atgtgttctc
     1981 ccgcacgtct cttaccgcat cctacaccgg ccgagtcgag atcgtgatga cgcaggaagc
     2041 gggttcgtgg gccgtgcgcg aatggcggga ttacgtcgtg tccggtgagt ccaatacctg
     2101 ggggctgtta cgagcccgat tgctcggttc gggatgaaat tgggctgagg tgcaaccggt
     2161 g
//
LOCUS       DAOTNG010000433         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39830_length_2152_cov_4.955174, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000433 DAOTNG010000000
VERSION     DAOTNG010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39830_length_2152_cov_4.955174"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="P5179_14140"
     CDS             complement(<1..1303)
                     /locus_tag="P5179_14140"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRS96405.1"
                     /translation="MPRKKKLTTVVGAPVVDNQNLMTAGPRGPGLLQDVWFLEKLAHF
                     DREVIPERRMHAKGSGAFGTFTVTHDITKYTKAKIFSKIGKKTDCFVRFSTVAGERGA
                     ADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPKTNLRSAK
                     NNWDFWTSLPEALHQVTITMSDRGIPASYRHMHGFGSHTFSFINAKNKRYWVKFHFVC
                     QQGIKNLTDAQAEAIIAKDRESHQRDLFDAIEKGDFPKWKLFVQIMTEEQAAKMPYNP
                     FDLTKVWYHKDYPLIEVGIMELNRNPENYFADVEQAAFNPANVVPGIGFSPDKMLQGR
                     LFSYGDAQRYRLGVNHNLIPVNAPRCPVNSYHRDGQMRVDGNAGATLGYEPNSYGEWQ
                     EQPEFAEPPLKITGAADHWNFREDDSDYYTQPGMLFRIMTAA"
     gene            complement(1380..1847)
                     /locus_tag="P5179_14145"
     CDS             complement(1380..1847)
                     /locus_tag="P5179_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRS96406.1"
                     /translation="MDPAQDDIKKRLLTFEEGLKAAGIRLTPQRLEIIRVLAESNDHP
                     DAETIHRTLRARMPTLSPDTVYRTLWLLLDLGLITTLGSSRGKARFDGNLAPHHHFVC
                     TKCGLARDFTYPDYDRLSIPDVVKTFGSVERMEVEIRGVCADCARRGGPISRA"
BASE COUNT          400 a          633 c          634 g          485 t
ORIGIN      
        1 gcgccgcggt catgatgcgg aacagcatcc cgggctgggt gtagtagtcg ctgtcatcct
       61 cgcggaagtt ccagtgatcc gctgctccgg tgatcttgag cggcggctcg gcgaactccg
      121 gctgctcctg ccactcgccg taactgttcg gttcgtaccc cagagtggct ccggcgttgc
      181 cgtccacacg catctgcccg tcgcggtggt agctgttcac cgggcagcgc ggtgcattca
      241 cagggatcag gttgtggttc acgccgagcc ggtagcgctg cgcgtcgccg taggagaaga
      301 gccggccctg cagcatcttg tcgggtgaga atccgattcc cggcacgacg ttcgccggat
      361 tgaacgcggc ctgctcgaca tccgcgaagt agttctccgg gttcctgtta agttccatga
      421 tacctacttc gatgagcggg taatccttgt gataccacac cttcgtgagg tcgaacgggt
      481 tgtacggcat tttcgccgcc tgctcttccg tcatgatctg cacgaacagc ttccacttcg
      541 ggaaatcgcc cttttcgatg gcgtcaaaca ggtcgcgctg atggctctcc cggtctttgg
      601 cgatgatggc ctcggcctgc gcatcggtca agtttttgat tccctgctgg cacacaaagt
      661 ggaatttcac ccagtagcgc ttgtttttcg cgttgatgaa gctgaacgtg tggctgccga
      721 acccgtgcat gtggcggtac gacgccggga taccgcggtc gctcatcgtg atggtgacct
      781 gatggagcgc ttccggcagc gatgtccaga aatcccagtt gtttttcgcg ctgcgcaggt
      841 tcgttttcgg atcccgtttg accgcgtggt tgaggtccgg gaacttgagc gggtcgcgca
      901 ggaagaacac cggcgtgttg ttcccgacaa gatcccagtt gccttcctcg gtgtagaact
      961 tgacggcgaa accgcggata tcccgctctg catcagccgc gccgcgctca ccggccaccg
     1021 tggaaaaccg cacgaagcag tcggtcttct tgccgatttt cgagaatatc ttggcctttg
     1081 tgtatttggt gatgtcgtgc gtgacggtga acgtgccgaa cgcgcccgat cccttcgcgt
     1141 gcatacggcg ttccggaatc acttccctgt cgaaatgggc gagtttttcg agaaaccaga
     1201 cgtcctgcaa caggccgggt cccctcggcc ctgccgtcat caggttctgg ttgtcaacga
     1261 cgggtgcccc caccaccgtg gtgagtttct ttttcctggg catggtcttc cctccttggt
     1321 tggcgcaatc ggtgatgaag cctgaggcgc cacagccacg ccgcggcgcc gcgagtgact
     1381 tacgcgcgtg aaatcggacc acctctccgc gcacaatcgg cgcagacgcc ccggatttcc
     1441 acctccatcc tttcgacact tccgaacgtt ttcaccacgt ccggaatgct cagccggtca
     1501 tagtccgggt acgtgaaatc gcgcgcaaga ccgcactttg tgcagacgaa gtggtggtga
     1561 ggcgccagat tcccatcgaa ccgcgccttt ccccgagatg accccagtgt ggtgatgagc
     1621 ccgagatcga ggagcagcca aagggtgcgg tacacggtgt ccggcgacaa agtcggcatt
     1681 ctggcacgaa gggtacgatg gatcgtctcc gcatccgggt ggtcgttcga ctctgccagc
     1741 acccggatga tctccagtcg ctggggggtt aggcgtatgc ccgccgcctt aaggccctct
     1801 tcaaaggtga gcaaccgctt tttgatatca tcctgtgcag ggtccacact gcccctttca
     1861 agaataagaa aaaaaattag ataggaataa ttactaaata agaacatagc atgaagaacg
     1921 agacgctgca aggcttgccg cccgaagcgc gcaaggtttt ccgcaccaac ctcgttacga
     1981 aattttccgc agtttcccct tgacacgaag acgacgtttt gcctaatcaa gagacgggcc
     2041 ggcgagggaa gaccgctgat gtcgtagcgg cggatcgcct ccaatacacg atggtgccgg
     2101 tgcgtgatag cgaattgcgg gtgagcgtgg tctgaatgtc gtcttctcat gc
//
LOCUS       DAOTNG010000434         2151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_39855_length_2151_cov_5.736164, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000434 DAOTNG010000000
VERSION     DAOTNG010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39855_length_2151_cov_5.736164"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2151
                     /locus_tag="P5179_14150"
     CDS             <1..>2151
                     /locus_tag="P5179_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS96407.1"
                     /translation="IQLDQRDAVAGRLVWRDVDNDGRFDLVAMGDSSATVSGIRLYRN
                     NPDLTLSRLSTSLPALGDGAFSLGHLDTDGTVDLIASGRLTPAGASQTTAWSLSGVPA
                     NALPTTPSLSPRENRPLVAADRVYFTWGQSPDDANGKTVSYEILLLQGVGTETRVLTA
                     PAAFGPAPHGRKLTYTLLRSLPPGSAMPLYVRARDAAGNTSPWSAPITVYVQDFVNSL
                     ETIVPLRSTAASWVDINGDGAPDLVVNGFDQGSNETNRLYVNQGGTLVPNTAYDLPGV
                     YLGSQAWGDVNADGYPDVMLTGSESEQVRITSIFLNRALQSVGLFDENAMTSDKLTGS
                     RAAFGDIDNDGDVDFVVTGVSGAALKTLVGENTGVITAQGVVFNVRTLDLTFVDTPAK
                     GLKDGCISLVDFDQDGDLDLTIQGNTDNGTAAADDDFWAGYLFTFRNTGGMNFSMVNT
                     PAPAGTATIPAGSALLDQLGDGEHVWADVDNDGDPDLICVGFSRTTAEFVLDNVYWDA
                     SALRIYENTGGGNLVMKQAFPGLWKASLAVGDADNDGKVDILVSGYNDHVVDTGGNHL
                     ETPTLRLYVNDGTGRFAEKSVALFSDYGVALGAVRFADVDGDGDLDAIAAGEGVTADG
                     KAVPKSTLFANTNAYFSANRRPNAPTGLAATVGTADSVRLSWNEAIDADPGTNKTPFH
                     TYALRAGTFPGRSNVRSGAEPVGRGTLGYMTHAT"
BASE COUNT          405 a          646 c          700 g          400 t
ORIGIN      
        1 aattcagctc gatcagcgcg atgcggtggc gggccgactc gtgtggaggg acgtggacaa
       61 tgacggccgc tttgatctcg tcgcgatggg cgactccagc gccaccgtga gcggcattcg
      121 gctgtaccgg aacaaccctg atctcacgct ttcccgtctg tccacctcgt tgccggcgct
      181 tggggacggc gcgttctctc tcggacatct ggacactgac ggcacggttg atctcatcgc
      241 gagcgggcgt ctcaccccgg caggggcgag ccagaccact gcctggagcc tttccggcgt
      301 gccggcgaac gctctcccga cgacgccctc cctttcgccg cgggaaaatc gcccgctggt
      361 ggcggctgac cgggtttact tcacctgggg ccaatcgccg gacgatgcaa acgggaagac
      421 ggtgtcgtac gagattctgc ttctgcaggg cgtagggacc gaaacccgcg tcctgaccgc
      481 gccggcggcc tttggtccgg caccgcacgg caggaagctc acgtacaccc tcctgcgctc
      541 gttgccgccc ggctcggcga tgccgctgta cgttcgagcg cgggatgctg cgggcaatac
      601 ctctccgtgg agcgcgccca tcacggtgta cgtgcaggat ttcgtgaact cgctggaaac
      661 gatcgttccg ttgcgcagta cggcggcctc atgggtggac atcaacggcg atggcgcgcc
      721 cgacctggtg gtgaacggtt tcgaccaggg aagcaacgaa acgaaccggc tttatgtgaa
      781 ccagggcggt actctggttc ccaacacggc gtatgacctt cccggtgtgt acctcggcag
      841 tcaggcctgg ggcgacgtga atgcggacgg ctaccccgat gtgatgttga cgggatcgga
      901 atctgagcag gtgcgcatca cgagcatctt cctgaaccgt gcgctgcaat cggtcgggtt
      961 gttcgacgaa aacgccatga catcggacaa acttaccggg agccgcgccg cattcggcga
     1021 tattgacaac gatggcgatg tggacttcgt cgttacgggt gtgtcgggag cggcattgaa
     1081 aacactggtg ggcgagaaca ccggcgttat caccgcgcag ggggtggttt tcaatgttcg
     1141 cacgctggat ctgactttcg ttgacactcc cgcgaagggg ctcaaagacg gttgcatctc
     1201 gctggttgac ttcgatcagg acggcgatct ggacctcacg attcagggca atacggataa
     1261 cggcactgcc gcggccgacg acgatttctg ggccggatac ctgttcacct tccggaacac
     1321 aggcgggatg aatttcagca tggtgaacac gccggccccc gccggaacgg caaccattcc
     1381 cgcgggaagc gcgttgcttg atcaactcgg cgatggcgaa catgtctggg ctgacgtgga
     1441 caacgatggc gatccggacc ttatctgcgt gggcttttcg cgaacaaccg ccgaatttgt
     1501 gctggataac gtgtactggg acgccagcgc gcttcgcatc tacgagaaca ccggcggcgg
     1561 caatctcgtg atgaagcagg cgtttccggg cctctggaag gcaagccttg ccgtcgggga
     1621 tgcggacaac gacggcaagg tggatattct ggtttcgggg tacaacgatc atgtcgttga
     1681 tacgggcggg aatcacctcg aaacgccaac gctgcggctt tacgtaaacg acggaaccgg
     1741 ccggttcgcg gaaaagtctg tcgctctgtt cagcgactat ggagtcgcgc tcggcgcggt
     1801 gcgattcgcg gacgtggatg gtgatggtga tctcgacgcc atcgccgcgg gtgaaggcgt
     1861 aacggccgac ggcaaagcgg tgccgaaaag caccctgttc gcgaacacaa acgcgtactt
     1921 cagcgccaat cgtcgcccga atgcaccgac cggtctcgct gccaccgtcg gtacggcgga
     1981 ttccgtgcgg ctctcgtgga acgaagccat tgacgcggac cccggcacga acaaaacgcc
     2041 gttccacacg tacgcactgc gcgcaggcac gtttcccgga cggagcaacg tccgttccgg
     2101 agcggagccg gtgggacggg gcacgctcgg gtacatgacc cacgcgacga t
//
LOCUS       DAOTNG010000435         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_40434_length_2127_cov_4.589286, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000435 DAOTNG010000000
VERSION     DAOTNG010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40434_length_2127_cov_4.589286"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="P5179_14155"
     CDS             <1..581
                     /locus_tag="P5179_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRS96408.1"
                     /translation="YGIAKELLSPAGYDAWVEGVATLIRNASQSELHPIPAAVNILGQ
                     LSRYSLGIIADQPAHLLSQFANWGFEGVFSVVALDSLTGFHKPDTRLFEWALTRANCR
                     AANAVMVGNRLDADVIPAKRSGMKAILCEIELSTKGWYPPGGDELLYLQSLSRVPNWR
                     NNTAELSAGEQPDGFVTRLSELPGVVEKLLAE"
     gene            596..1294
                     /locus_tag="P5179_14160"
     CDS             596..1294
                     /locus_tag="P5179_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRS96409.1"
                     /translation="MEKEPEGRVEFLQRVCGAVARRAPERPTSGPSEFTREVGRSVRD
                     GQLADIFVGNARAAGIDARIVPTRTMVVDAVNDITRTYSVRTLHAWNDGTVERYGLES
                     LAEMSGLEIQRWNPEQPEESRKAAAFGMQCGLTSADYAIAETGSLVLTTSREKGRSVS
                     LLGDVHIAIVETDRLIPDLYDLPQRLLRDHPTLLPSNVTIISGPSKTADIELQMVVGV
                     HGPRHVFAILVQSD"
     gene            1460..>2127
                     /locus_tag="P5179_14165"
     CDS             1460..>2127
                     /locus_tag="P5179_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96410.1"
                     /translation="MEKTDPLLGAIGAVRERHIVHNLRRIFEIIASVERGDSTLEKTA
                     QSYFRSHPKLGSSDRALIGDVVITTYRWKMRLQNALRHTLVQERPTRFRAEETDTLLP
                     GAVLISSGYDANVVTSAYRRVFPSLDLPPLFWEQLASHVLSLNPWESADLEQLALRAS
                     LPEWVVRRLTADHGFERARSLAIALQTKAPITLRNNDLRKDRETLIAELLAAGVAVVP
                     TVFSP"
BASE COUNT          451 a          595 c          624 g          457 t
ORIGIN      
        1 attacggcat cgcgaaggaa ttgctttcgc ccgcggggta cgacgcctgg gtcgaaggtg
       61 tggcaacact catccggaac gcgtcacagt cagaacttca ccccatcccc gccgcggtga
      121 acattctcgg gcagttatcc cggtactcgc tcggtatcat cgcggatcag cccgcacatc
      181 ttctcagtca gttcgccaac tggggatttg agggagtctt ttcggttgtc gctctggact
      241 ccttgaccgg ttttcacaaa ccggacacac gcctgttcga gtgggcactc accagggcga
      301 actgcagggc ggcaaacgct gttatggtgg gcaatcgcct tgacgcagac gtcattcctg
      361 caaaaagatc gggcatgaag gcaatccttt gcgagattga gctttcgacg aaagggtggt
      421 atccaccggg cggcgatgag ttactttatt tgcaatccct ctcgcgcgtt ccgaactggc
      481 ggaacaacac agcggaactc agtgctggtg aacaacccga cggttttgtc acccggctga
      541 gcgaactacc aggtgtggtg gaaaaactcc tcgcggaatg aaggaacagg taggaatgga
      601 aaaggaaccc gaagggcgtg tggaattcct tcagcgggtc tgcggagcgg ttgccaggag
      661 agcacctgaa cggccaacgt ccggcccttc cgaatttacc agggaagtgg gccgaagcgt
      721 tcgcgatggg caactggcgg atatattcgt cggaaatgcc cgtgccgcag ggatcgacgc
      781 gcggatcgtc ccgacgcgca cgatggtagt ggatgcggtg aacgacataa ctcgaacgta
      841 ttccgtccga acgcttcatg cgtggaacga cggaaccgtg gaacggtacg gcctggagtc
      901 tcttgcagag atgtcggggc tggaaatcca gcggtggaac cccgaacaac cggaggaatc
      961 gagaaaagcc gccgcatttg ggatgcaatg cgggctgacc tctgcagact acgcgattgc
     1021 ggaaactggc tcccttgtgc tcaccaccag ccgtgagaaa ggaaggtcag tcagtctgct
     1081 gggcgatgtc cacatcgcga ttgtcgaaac ggacagattg atacctgatc tctatgacct
     1141 gccgcagcgc ctcctgagag atcaccctac tctgttgcct tccaatgtga caatcatttc
     1201 aggaccttct aagaccgcag acatcgagct gcagatggtc gtaggagtac acggtccgcg
     1261 gcacgtgttc gcaatcctcg ttcagagtga ttgatcattg catccgctgc cagcgcgggc
     1321 gcgcgaagct ggtcgtggtc gggatgcggg tccagtagct ctcggcgcgg accgcatgtt
     1381 gtcttctttc cctccacgta tcttgctttg ggcgtgaagg cagtgctttc gtcctgcgcg
     1441 tgacatcacg aggctcttga tggagaagac cgatcccctc ttgggagcaa ttggcgccgt
     1501 ccgcgaacgg catatagtcc acaacctccg gcgcatcttc gagatcattg cgagtgtgga
     1561 acgcggcgac tccactcttg aaaaaactgc tcagtcgtat ttccgttcac acccgaagct
     1621 gggcagttct gatcgggcac tgattggtga cgttgtcatt acaacgtacc gttggaaaat
     1681 gcgcttgcaa aacgctctgc ggcatacact tgtgcaggaa cgtccaactc gattccgggc
     1741 agaagaaact gacacgttgc ttcccggggc ggtgctcatt tcgtcgggat atgatgccaa
     1801 cgtggtaacc tccgcttatc gccgtgtctt cccttcactc gatcttcctc ccctgttctg
     1861 ggaacagctc gcttcccacg ttctctcgtt gaatccctgg gaaagcgcgg acctggaaca
     1921 gttggcctta cgggcttcgc tgccagaatg ggtagtacgg agactcacgg cggatcatgg
     1981 cttcgagcgg gcaaggtctc ttgcgatcgc gcttcagacg aaagcaccca tcacgttgcg
     2041 taacaatgac ttgaggaaag acagagaaac gctgatcgcg gagctcctcg ccgcgggagt
     2101 cgctgttgtg cctaccgtgt tcagccc
//
LOCUS       DAOTNG010000436         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_40491_length_2125_cov_4.067633, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000436 DAOTNG010000000
VERSION     DAOTNG010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40491_length_2125_cov_4.067633"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="arsM"
                     /locus_tag="P5179_14170"
     CDS             <1..777
                     /gene="arsM"
                     /locus_tag="P5179_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRS96411.1"
                     /translation="KRYAEIATSGATSCCSSSTCCSGTPGEKADATQAALAAGYTLEE
                     LATLPEGANMGLSCGNPLVLASIGEGEVVLDLGSGGGLDVFLAGNIVGAGGRAIGVDM
                     TPEMVTKARNNIAAYTERTGLNNVEFRLGEIEHLPVADESVDVIISNCVVNLSLDKAA
                     VFNECFRVLKPGGRLSISDIVADKPLPESMRTDDDLVCGCIGGAASVDEVRAWLENAG
                     FQDIRVEIEEESRELIGRWVPGSGIENSVASAIIRGRKPE"
     gene            872..1756
                     /locus_tag="P5179_14175"
     CDS             872..1756
                     /locus_tag="P5179_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRS96412.1"
                     /translation="MNPFAVSLKFFVFILAELTALFLGISTLVGLMLQYVSDEKLRTW
                     LSRKGVLGNFLGAALGAITPFCSCSTIPMTVGLLRAGVPFGATMSFVLASPLLNPIIL
                     TMFLFLLGWKACVVYGAVTFVASMACGAALQGFGLASDVRNVRVSGVVHDQEKPETFR
                     GKLREAFAGAWGDFRGVLVYLVIGVAIGAAIYGYLPGDFVVGIAGPQNPFAIPVAAVI
                     GVPLYVRAETVIPIAVALTQKGMSLGAVIALIIGGAGMSIPEMSMLASIFKPRLVATF
                     VSVVLVTAVLAGVVFNVI"
     gene            1885..2118
                     /locus_tag="P5179_14180"
     CDS             1885..2118
                     /locus_tag="P5179_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRS96413.1"
                     /translation="MATYVYETIPGKPGEKPKRFEIQQRMSDEPLVTHPETGEPVRRV
                     ISGGLGTITSRGKQADGSRGERTLQCSCGGNCE"
BASE COUNT          426 a          538 c          700 g          461 t
ORIGIN      
        1 aagcgttacg ccgaaatcgc gacttccggc gccacatcct gttgctcgag ctctacgtgt
       61 tgttccggaa cccccgggga gaaagctgac gcaacccagg ccgctctggc tgccggatac
      121 acgctggaag aactggccac gctcccggaa ggcgcgaaca tggggttatc ctgcggcaat
      181 ccgctcgtgc tcgcctcgat cggggaaggc gaggtcgttc tcgacctcgg cagcggcggg
      241 ggccttgacg tattccttgc gggcaatatc gtcggggcag gaggccgcgc catcggcgta
      301 gatatgaccc cggagatggt gacaaaggcg cggaacaaca tagcagcgta cacagagcgc
      361 acgggcctca acaacgtgga gttccgactg ggcgagatcg agcaccttcc cgttgccgac
      421 gagagcgtag atgtcattat atccaactgc gtcgtgaatc tgtcgctgga caaggctgcg
      481 gtgttcaacg aatgcttccg tgttctgaaa cctggcggac ggctttcgat ttcggacatc
      541 gttgcagaca aacctctgcc cgagagcatg cggacggatg atgacctcgt gtgcggctgt
      601 atcggcgggg cggcctcggt ggatgaggtt cgtgcatggt tggagaacgc tggattccag
      661 gacatccgtg ttgagattga ggaagaaagc cgcgaactga tcggccgctg ggtacccggg
      721 agcggaatcg agaactctgt cgcatcagcg atcattcgcg ggagaaaacc tgaatagctt
      781 ttgcctgttg tcacggttcc ggtgaagcaa ccggttgtcc gatagcgacg gtccgggatt
      841 ggaactgggt aaacgatgaa aaggtggata gatgaacccg tttgcagtat ccctgaagtt
      901 cttcgtcttc attctcgcgg aactcacagc gctgttcctc ggcatcagca cgttagtggg
      961 tctgatgctc cagtacgtgt ctgacgagaa actccgcacg tggctttcga ggaaaggtgt
     1021 gcttggtaat ttcctcggtg cggcacttgg agcgattacg cctttctgct cgtgttccac
     1081 gatcccgatg actgtcgggc ttttgagggc aggcgtgccc tttggtgcga cgatgtcatt
     1141 cgtacttgcg tcgccgttgc tgaatccgat catcctgacc atgttcttgt ttctgctggg
     1201 gtggaaagcc tgtgtggtat acggggccgt aacgtttgtt gcatccatgg cttgcggagc
     1261 ggcgttgcaa ggcttcggat tggcatcgga cgtcaggaat gtccgggtga gcggtgtggt
     1321 gcacgatcag gagaaaccgg aaacgttccg gggaaaactc cgcgaggcct ttgcgggagc
     1381 gtggggggat ttccgcgggg tgctggtgta cctcgtaatc ggcgttgcga tcggcgcggc
     1441 gatttacggt tatcttccgg gtgatttcgt cgtcggtatt gcgggcccgc agaatccatt
     1501 cgctatccct gttgcggcag tgatcggtgt tccgctgtat gtccgtgcgg aaacggtgat
     1561 tcctatcgcc gtggccttga cccagaaagg catgagcctc ggggcagtaa tcgctttgat
     1621 tattggcggg gccggcatga gcatccctga gatgagtatg ctggcgagca tcttcaagcc
     1681 tcgcctggtg gcgacgttcg tttcagtagt gttggttacg gcagtgctgg cgggtgttgt
     1741 gttcaacgtg atctgaagga gagtggattg atggctgaca ggaagggcaa aggggtgttg
     1801 tccgtgttgt ttggaccgaa gaaatcctcg tgctgcaacg tacagatcga agaagtcacc
     1861 gaagacgcca cagagggcga cgcaatggct acctacgtgt atgaaacgat ccccggcaaa
     1921 ccgggggaga aaccgaaaag gttcgagatc cagcagagga tgagcgacga accacttgtc
     1981 acgcatccag aaaccggcga gcccgtcagg cgagtaatct ccggagggct cggaacaatc
     2041 acgtccagag ggaaacaagc ggacggaagt cgaggggagc gcacgctgca atgctcctgt
     2101 ggcggcaact gcgagtgagc ggccc
//
LOCUS       DAOTNG010000437         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_40754_length_2115_cov_4.897087, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000437 DAOTNG010000000
VERSION     DAOTNG010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40754_length_2115_cov_4.897087"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="P5179_14185"
     CDS             <1..228
                     /locus_tag="P5179_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS96414.1"
                     /translation="RCLYSRNIVNLFMAGRNISTTYWALGAVRVMRTCGLMGEVAGTA
                     AAVCARHGCLPREVYKTYLGELKRLLGASST"
     gene            288..575
                     /locus_tag="P5179_14190"
     CDS             288..575
                     /locus_tag="P5179_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96415.1"
                     /translation="MRTRAMAVEAARLDAAVRALRKMGATRVLLFGSFAESPETARDI
                     DIAVEGIPLNRILDADVAVHDLLGVPVDLVSREETPEFYSLISRKATVLYE"
     gene            568..1047
                     /locus_tag="P5179_14195"
     CDS             568..1047
                     /locus_tag="P5179_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96416.1"
                     /translation="MSNQANIREEIHFELEQLRQVADLASELSSVLPEQRRPWDVAAA
                     AKIVADFSVGFENMCRRRYRYLGKPLPEGPDSHSVMLNDFVAVHGPGSSLAPELKERL
                     RKYLRFRHRFVHSYGLRMRWEIVDEPLRLLPDTVSQLSKVWEAWLESLSPEQGGGQL"
     gene            1044..2000
                     /locus_tag="P5179_14200"
     CDS             1044..2000
                     /locus_tag="P5179_14200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS96417.1"
                     /translation="MISVVIPCYNEEEVLPQLFARLGAAAETWGEEYEILLVDDGSRD
                     ATWQLILEQHARDERWRGISFARNFGHQTAISAGIHYARGCAVILMDADLQDPPEQLH
                     RFIAAWRDGNDVVYAIRATRKENILKRAAYKAFYRVLRRLASIEIPLDSGDFCLMDRK
                     VVDVLNAMPERNRFVRGLRSWAGFRQLGLEYNRDARAAGEVKYTLRKLIKLALDGVVS
                     FSTTPLRMATYLGFVVSFFALLGAAVTILQRIFVEWFTRIGFPYVPGFYALFTSMLFL
                     GGVQLISLGIIGEYLGRIFDEVKGRPLWTIRETAGLEMDAPK"
BASE COUNT          394 a          574 c          699 g          448 t
ORIGIN      
        1 cgctgcctgt acagccgcaa catcgtcaac cttttcatgg ccggccggaa tatcagcacc
       61 acgtactggg cgctcggggc ggtgagggtg atgcgcacgt gcgggttgat gggtgaagtt
      121 gcgggtaccg cggcggccgt gtgtgcccgc catggttgtc ttccgcgtga ggtgtacaaa
      181 acgtatcttg gagagttgaa acgacttctg ggtgccagtt ccacataatg tcgaggcagg
      241 caccgtgtga cgaaccagga atgatacggg tctgcggggg cggtccgatg cgaacgcgag
      301 ccatggcagt tgaagcggcg agactggatg cggcagtgcg tgcgctccgg aagatgggtg
      361 ccacacgggt gcttcttttc gggagcttcg cggaatcgcc ggagacagcg cgtgacatcg
      421 atattgcggt ggaaggtatt ccgctcaacc gcatactgga tgccgatgtc gccgttcacg
      481 accttctggg cgtgccggta gacctcgtaa gtcgggagga aacgccagaa ttctactcgc
      541 tgatcagccg aaaagcgacg gtgttgtatg agtaaccagg caaacattcg tgaggaaatc
      601 cacttcgaac tggagcaact caggcaggtg gcggaccttg cgtctgagtt gagttcggtt
      661 ctgcctgaac aacggcgtcc gtgggatgtg gccgccgccg cgaagattgt tgccgatttc
      721 tccgttgggt ttgagaacat gtgcagaagg cgataccggt acttaggaaa gccactgccg
      781 gagggtccgg actcccacag cgtcatgctc aacgatttcg tggctgtcca cgggcccggt
      841 tcctcgctgg cccccgagct gaaggagcgt ctgcgcaaat acctccgatt tcggcatcgt
      901 tttgtacaca gttacggact gcgaatgcgg tgggagatag tggacgagcc gcttcgtctg
      961 cttccggaca ccgtcagcca gctttccaag gtctgggagg catggcttga atcactttca
     1021 cccgagcaag gtgggggaca gctttgattt ccgtcgtgat cccctgctac aacgaggagg
     1081 aggtgcttcc acagctcttc gcgcgactgg gtgcggcggc ggagacgtgg ggcgaggagt
     1141 atgagatcct tctcgtggac gatggttcgc gggacgcaac ctggcagctg atcctcgaac
     1201 agcatgcgcg ggatgagcgg tggcggggga tttcatttgc aaggaatttc ggccatcaga
     1261 cagccatctc cgccggcatt cattacgcgc ggggctgcgc cgtcatcctg atggacgcgg
     1321 atctgcagga tccacctgaa caactccacc gtttcatagc ggcgtggagg gacgggaacg
     1381 acgttgtgta tgccattcgg gctacgcgga aggaaaacat cctgaaacgc gcggcgtaca
     1441 aggcgtttta ccgcgtcctg cggcggctcg caagcatcga aatcccgctc gacagcggcg
     1501 atttctgtct gatggatcgc aaggtggtgg atgtcctgaa cgcgatgccg gaacgcaacc
     1561 gtttcgtgcg ggggctgcgc tcgtgggcgg gcttccgtca gctcggtctc gaatacaacc
     1621 gcgatgcgcg tgcggcgggc gaagtgaagt acacgctgcg caaactgatc aagcttgccc
     1681 tcgatggtgt agtgtccttt tccactacgc cgcttcgcat ggccacgtac ctcgggttcg
     1741 tagtgtcctt ttttgcgctg ctgggggccg cggtaaccat tctccagcgg attttcgtgg
     1801 agtggttcac gcgcatcggc ttcccctatg ttcccggttt ctatgcgttg ttcacaagca
     1861 tgctgttcct cgggggcgtg caactgatct ccctgggtat catcggcgaa tacctcgggc
     1921 gcatcttcga cgaggtgaag gggcgcccgc tctggacgat ccgtgagacg gccggtctgg
     1981 agatggacgc accgaaatga cgtcgaaaga gcggcttctg acggcctacc ggtgcggaca
     2041 gccggatcgg gtgcctatca atgtccgcgg ggtaccggtg catgatcgga agtgggtcgg
     2101 aagccgtcac cccag
//
LOCUS       DAOTNG010000438         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_40780_length_2114_cov_4.886353, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000438 DAOTNG010000000
VERSION     DAOTNG010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40780_length_2114_cov_4.886353"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>2114)
                     /locus_tag="P5179_14205"
     CDS             complement(145..>2114)
                     /locus_tag="P5179_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HRS96418.1"
                     /translation="PPERVRRFLDTYPCIVGLQIDEASQRTFVNHGGGPEYTMGRNAR
                     YARDIIRLAAEYGCFMSWQLMRDNWAAIGCSADNEALYDAICEYSEYVIPMHEMNCEF
                     SKFINHLSCMGLWLAGATQQWGIEAQSWYWYDCGYNKPGTCEPGTLEMPGELYAIMFL
                     LGVSAGASVFSIEPPTDNWPGPGHWRFTEWIAPVFKRLLREHLIPSREEILASTPLAY
                     HLPRCERPVDYHRVLADLDFDHGEGRLIRVTYGVFDRARDAEFIPNNPRYGWIPVLPA
                     KTPESLLSRVPRVIRPGDIQSVEEARNVAEEEFPPVDRGQAWSVNAGRLLFAVNTHEN
                     WYVPESVKLSVPLRPDGVRLEDSGAVSLLRWNRHPGDRAYRVWRLREGVERCLTEYPV
                     QDTEYRIHEPANNDFYSVSAITDATELISGTLHLHQFLLFDCQESRRSRWISSSGESE
                     EHFRIGESMPPETEEIARAEARASQCSPVEDLASPPVAEDDPWAPQKRGVIEAMVGWK
                     SAVESEEIPRIMAFYAEDYREPDGRTRESVEVAFRNLFRRYVMDRFEPFLQEWGAVPG
                     WQFPALRLLIREWGEASSQTVEVSAVAHLWAGGGPELEPSDMIIIPFGRPSLTTFVWT
                     NAGRGWKLAKTAPAFLQVEDTAVFRFRYQGW"
BASE COUNT          436 a          648 c          592 g          438 t
ORIGIN      
        1 atgagcagag ggtgggacgg gcagatagtc cttttcaacg gcgttttcat gcattctcca
       61 tggtattggt ccagatttcg acgagcagtc gggttcgagt gagcaatcct gttcgtgtcc
      121 tgatgcattg cttgcagggc aggttcacca cccctggtaa cgaaatcgga aaaccgcggt
      181 gtcctccact tgaagaaacg cgggggccgt tttcgccagc ttccagccgc gccccgcgtt
      241 cgtccacacg aacgtggtca gcgacggccg tccgaacgga atgataatca tgtcactcgg
      301 ttcaagttca gggcctcccc ccgcccagag atgcgcaacc gccgatacct cgaccgtttg
      361 cgacgatgcc tcgccccact cgcggatcaa caggcggagt gccggaaact gccatccggg
      421 cactgcaccc cattcctgga gaaagggttc gaaccgatcc atgacatatc tgcgaaaaag
      481 gttcctgaac gcgacttcca cggattcccg cgtccttcca tccggttcac ggtaatcttc
      541 ggcgtaaaag gccatgatgc gcgggatttc ttctgactcc acggcagatt tccaaccaac
      601 catggcctca atgaccccgc gtttctgcgg tgcccaggga tcatcctccg cgaccggcgg
      661 cgaagcaaga tcctccacag gtgaacactg gctggcacgc gcctcagcgc gggcgatttc
      721 ctccgtttcc gggggcattg actcgccgat gcggaagtgt tcttcagatt ccccggagga
      781 gcttatccat ctgctgcggc ggctttcctg gcagtcgaag agcaggaact ggtgaagatg
      841 caacgtaccc gatatcaact cggtcgcgtc agtgattgcc gaaacggagt aaaagtcgtt
      901 gttcgcaggc tcgtggatac gatattctgt atcctggacc ggatactcgg tcaggcagcg
      961 ctccactcct tctctcaggc gccatacacg gtaggcgcga tcacccggat gacggttcca
     1021 ccggagaaga gacactgctc ccgagtcttc aaggcgcaca ccgtccggcc tgagcggtac
     1081 agacaacttg actgattccg gcacgtacca gttttcgtga gtattcacgg caaaaagcaa
     1141 tctgcccgcg ttcacgctcc acgcctgtcc gcgatcaacc ggtgggaatt cttcctccgc
     1201 aacgttccgt gcctcctcga ctgattggat gtctcccgga cgtattacgc gcggaacgcg
     1261 ggacagcaga gactccgggg tcttcgcagg caggacgggg atccatccgt accgcgggtt
     1321 gttcggaatg aactccgcgt cccgtgcgcg atcgaaaacg ccatacgtta ctcgtatgag
     1381 cctgccttca ccgtggtcaa aatccagatc ggccagcacc ctgtggtaat caacaggacg
     1441 ttcgcaccgt ggcaggtggt acgccagcgg cgtggatgcg aggatctcct cacgtgaagg
     1501 gatcaggtgc tcgcgcagga ggcgtttgaa cacaggcgcg atccactctg tgaaccgcca
     1561 gtggcctggc cccggccagt tgtcagtcgg gggctctatg gaaaacacgg atgccccggc
     1621 gctgactccc agcaaaaaca tgatggcgta caattcgccc ggcatttcca gtgtgcccgg
     1681 ttcgcacgtt ccgggtttgt tgtacccaca gtcgtaccaa taccatgact gggcctcaat
     1741 gccccactgt tgtgttgcgc cggcgagcca cagccccatg cacgaaagat ggttgatgaa
     1801 tttggagaac tcacagttca tctcgtgcat aggaatcacg tattccgaat actcgcagat
     1861 cgcatcgtac agcgcttcgt tatcggcgga acacccaatg gcggcccagt tatcgcgcat
     1921 gagttgccag ctcatgaagc acccgtactc ggcagcaaga cggatgatat cgcgcgcata
     1981 cctcgcgttc cggcccatgg tgtactcggg gccacctcca tggttcacaa atgttctctg
     2041 gctcgcctca tcaatctgga ggccgacgat gcaggggtag gtatccagaa agcgcctgac
     2101 cctttccggc ggca
//
LOCUS       DAOTNG010000439         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_41034_length_2104_cov_4.277208, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000439 DAOTNG010000000
VERSION     DAOTNG010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41034_length_2104_cov_4.277208"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5179_14210"
     CDS             complement(<1..215)
                     /locus_tag="P5179_14210"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRS96419.1"
                     /translation="MTVQRVLGVDPGTIVCGYGIIDVAGSRYTYVTSGVIRPLRNAPM
                     PDRLCTVFRGIASLIEEYRPTVLAVEEA"
     gene            complement(303..1052)
                     /locus_tag="P5179_14215"
     CDS             complement(303..1052)
                     /locus_tag="P5179_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRS96420.1"
                     /translation="MSGHSKWATIKRKKGKNDAQRGRIFTKLIKEIAIAARNGGGNPD
                     ANPRLRRAIDTAKANSMPMENVERAIKRGTGELEGVSYEEITYEGYAPGGVALLIESV
                     TDNRNRTVSELRNLLSKNGGNLAEAGAVAWVFEAKGSISVEKSAGTEEDVFNIALEAG
                     AEDMETEGETYEITTPLSELDAVRRALEDAGIAVASAEPARVPTTTVKVEGKQAEQVM
                     RLLEALDDHDDVQKVWDNSDIDEKVLAELAA"
     gene            complement(1630..>2104)
                     /locus_tag="P5179_14220"
     CDS             complement(1630..>2104)
                     /locus_tag="P5179_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS96421.1"
                     /translation="QGTLMQTLLCHSAGVMSLAVAPDGRNVISGGADAGVSSGTVQAA
                     LWRWAIAPPLGVDPSAGDNNPRQFTLSQNAPNPFNPSTTLRFGLPEAGHVTLAVYDVR
                     GALVCTLVDQTLVSGVHAVQWDGYDAMGREVASGVYLARLTAPQGVVTRRMVLLR"
BASE COUNT          417 a          689 c          604 g          394 t
ORIGIN      
        1 gcctcttcca ccgcaagcac ggtggggcgg tactcttcaa tgagcgatgc aattccccgg
       61 aacacagtgc acaggcggtc tggcatgggt gcgtttcgga gcgggcgaat gaccccggag
      121 gtcacatacg tataccggct gcctgcaaca tcgataatac cgtacccgca gacgattgtt
      181 ccagggtcaa cgccgagaac ccgttgtacc gtcatcagga aacctgcaga aacgaagaac
      241 cccgcgcatc gacctcgcgg cagagaaagc cgtgccggta gtacgcgcgg ggaaccgttg
      301 aatcaggccg caagttcagc cagaaccttc tcgtctatgt cagaattatc ccagaccttc
      361 tggacatcgt catggtcgtc gagtgcttca agcaaccgca tgacctgttc tgcctgcttc
      421 ccctcaactt tcaccgtggt ggtgggaacg cgcgccggtt ccgcactcgc aaccgcgatg
      481 cccgcgtctt caagggcgcg acgaactgca tccagttcgc tcaagggtgt tgtgatctcg
      541 tatgtttcgc cctcggtttc catgtcctcc gccccggctt ccagcgcgat gttgaacaca
      601 tcctcttctg tgccggcgga tttttccacc gagatgctcc ccttcgcctc aaacacccat
      661 gcgaccgcac ccgcttccgc gagattaccg ccattcttgc tcaacaggtt tcggagctcc
      721 gaaacggtgc gattgcgatt atctgtcacc gattcgatga gaagagcgac accgccgggg
      781 gcatatcctt cgtaggtgat ctcctcgtac gaaactccct cgagctcgcc cgttcctcgc
      841 ttgatcgccc gctcgacgtt ctccatcggc atgctgttcg ccttggcggt atcgatcgca
      901 cggcgcaacc gagggttcgc gtcggggttg cccccgccgt tccgggcggc aatggcgatt
      961 tccttgatca acttcgtaaa aatgcgcccg cgctgggcgt cattcttccc cttcttgcgc
     1021 tttatggtcg cccacttgga atgtcctgac atggcattac tcctgaatcc ggttcgacaa
     1081 cgacggcgaa cgatcaacca cgaacgacac caggccgttc agagggaggg cgcctgtgcg
     1141 tatttcgaac cagaagaaga ccaaaacaac gaggcgcgac aggaaagcct cgtccgacag
     1201 gacaactgca catgaaatcc atctccagcg caggggaatg aacgcgcgcg tagcaggtac
     1261 caaaagtacg gtttcacacc ggtattcgcc aagatgcgcc ggattgcgcg attcgcccgg
     1321 ctgaagccgt agtactgcgg gggaacggca gggacaaggc cggaacgcgg cagtagaagt
     1381 ggaaaaccag acacgcagtc gttggcgagt ggtgtggatt gcgcatgttg gcgcgtcgcc
     1441 agccaagctt gccggcgcag gcattttgac gcacacacgg gaccgccact acaccaggaa
     1501 ccgccctacc tccagacaca ccttagcgcg gtcgttgagc ctgtcgaaac gccgcgcggg
     1561 gcttcgacag gcttcctgcg cgacgcacac gctcagccac cacgcgctcc tacctcacca
     1621 ggctgcgcgt cagcgtagca gcaccatccg cctcgtcacc acgccctgtg gggcggtcag
     1681 gcgcgccaga tacaccccgc tggcgacctc acgccccatt gcgtcatacc cgtcccattg
     1741 gaccgcatgg accccggaaa ccagtgtctg gtccaccagt gtgcaaacca gcgcaccccg
     1801 cacgtcgtac actgccagcg tcacatgccc cgcctcgggc aggccgaacc gcagcgtggt
     1861 ggacgggttg aacgggttgg gcgcgttctg ggagagggtg aactgccgcg gattgttgtc
     1921 tcctgcgctt gggtcaacgc caagcggggg ggcaatcgcc caacgccaga gggccgcctg
     1981 aaccgttccg gaggatacgc cggcatcggc cccgccggag atgacgttcc tcccgtcggg
     2041 agcgacggcc aagctcatca cgccggcact gtggcaaagc agcgtttgca ttagtgtgcc
     2101 ttga
//
LOCUS       DAOTNG010000440         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_41189_length_2097_cov_4.873653, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000440 DAOTNG010000000
VERSION     DAOTNG010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41189_length_2097_cov_4.873653"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="P5179_14225"
     CDS             <1..1256
                     /locus_tag="P5179_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS96422.1"
                     /translation="NTWVHLPPAQNGMPSPPAGEPDGGFISIAVDRQNRIWAGTWGGG
                     IGVVSGQGTTPLHAVTVSTANSPLTGLDGSPDYLVTKAFHSGVDGVMYAGIWHSGVFA
                     LSPGFAPGSGTTYQIMRKFMENGTISRTLNVTALTLDRYGVLWVGTSSAGVLLIDTNS
                     TPLDGSDDQFVGTLGRQGIDSDVGLRSRNVRSIARARNGEIWVATDAGLTLFQGEFAR
                     ATNRYSLTTRHFTLDDGLPSNSLNAVLVDSFDVVWVGTDKGLAQVLPTKQVITLPVER
                     LVDTEGKVTSLAFDRARGYLWIGTPSGLARYEAYPPVGAGDSIIAEPSQNPFMIGISQ
                     RGSDYVLTGTPLSIVVTPGATVRIYTITGDLVWEATDSGIGQVTWDGRTRSPARAVAS
                     GVYLYIAQKGSNTATGKIAVLRDAR"
     gene            complement(1273..>2097)
                     /locus_tag="P5179_14230"
     CDS             complement(1273..>2097)
                     /locus_tag="P5179_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRS96423.1"
                     /translation="YDLMGLFIGSEGTFGIATRLFLRLERAPEAVRTVLADYTSIAEA
                     CQTVSDIIAGGMVPTALEIMDRRTIGTVEASALAAGYPVDAEAVLIIEVDGLPYSLDD
                     QVDRITEICRKNGARSVRFASDESERKRLWAGRKAAFGASGRISPDLLVQDAVVPRTR
                     LAEIVPRITEICDRHGVKVSNVFHAGDGNLHPNIGYDRRNTEEARRVKDALAEIMAIC
                     VATGGTITGEHGVGLEKVDFMPLYFDRATREAMSRVRSVWDPTGLCNPGKVLSSQD"
BASE COUNT          405 a          644 c          629 g          419 t
ORIGIN      
        1 acaacacctg ggttcatctt ccccccgccc agaacgggat gccatccccg cccgccgggg
       61 aacccgatgg cggattcatt tcgattgcgg tggacaggca aaacaggatc tgggcaggaa
      121 cctggggcgg cgggatcgga gtggtttcgg gccagggtac cactccgctt cacgcggtga
      181 cggtgagcac cgccaacagc cctctgacgg ggctcgacgg cagccctgat tacctcgtca
      241 cgaaggcctt ccactccggc gtggacggag tcatgtacgc gggcatctgg cacagtggag
      301 tgttcgccct ttcccccggt ttcgcccccg gcagcggcac cacataccag atcatgcgca
      361 agttcatgga gaacgggact atttcgcgca cgctgaatgt gacggcactc acgctcgacc
      421 gatatggcgt gttgtgggtt ggaacttcgt cagcgggtgt gcttctgatt gacacgaaca
      481 gcactccttt ggatgggtcg gacgaccagt tcgtcggcac actggggcgc cagggaatcg
      541 actccgatgt tgggttgcga agccggaatg tgcggagtat cgcacgtgct cggaacggag
      601 agatctgggt cgctacggat gccggtttga cgctcttcca gggcgaattt gcccgcgcaa
      661 ccaatcggta cagcctgacg acccgccatt tcactctcga tgatggtctg ccatcgaaca
      721 gcctgaacgc ggtcctcgtg gactcattcg atgtcgtgtg ggtggggacg gataaagggc
      781 tcgcccaggt tcttcccaca aagcaggtga tcaccctgcc cgtcgaacgc cttgtcgata
      841 cagagggaaa ggttacctca ctggcatttg accgggcacg gggctatctg tggattggaa
      901 cgccttccgg cctcgcgagg tacgaagcgt accccccggt tggcgcaggc gacagcatca
      961 ttgcggaacc ctcacagaac cccttcatga ttggaatctc ccagcggggc tcggactacg
     1021 tgcttacggg cacgccgctc tcgatcgtcg tcacgcccgg tgcgacggta agaatttaca
     1081 ccattacggg ggatctggtg tgggaggcga cggattctgg catcgggcag gtaacgtggg
     1141 acggaagaac gcggtcaccc gcccgggcag ttgcttccgg cgtctatctt tatatcgcgc
     1201 agaagggatc gaacacggcc accggcaaga ttgccgtcct gcgagatgcc cgctgaactg
     1261 cgcgcgccgc cgtcaatcct gactggaaag aacctttccg gggttgcaga gccccgttgg
     1321 atcccagacg gatcgcacgc gcgacatggc ttcccgggta gctcggtcga agtacagagg
     1381 catgaaatcg accttttcca gacccactcc gtgttcgccg gtgattgtac cgccggttgc
     1441 cacgcagatt gccatgattt cggcaagcgc gtccttcact ctccgcgcct cttccgtgtt
     1501 gcgtctgtca tacccgatgt tgggatggag attcccgtcg ccagcatgaa acacgttgga
     1561 gaccttcacg ccgtgccggt cgcagatctc agtaatccga ggcacaatct cggcgagccg
     1621 cgtacgcggc actacggcgt cctgcaccag tagatcgggg ctgatgcgcc ccgacgcgcc
     1681 aaaagcggcc ttcctgcccg cccagaggcg ttttcgttct gattcgtccg atgcgaaccg
     1741 tacgctgcga gcgccattct tccgacagat ttccgtgatc ctgtccacct gatcatcaag
     1801 gctgtacggc aacccgtcga cctcaatgat cagcaccgct tccgcatcga caggataccc
     1861 cgccgcgagt gccgatgcct ccacggtacc gattgttcgg cggtccatga tctccaatgc
     1921 agttggaacc attcctccgg ctatgatgtc ggaaaccgtc tgacacgctt ctgcgattga
     1981 cgtatagtcg gcgagaacgg tcctgacagc ttcaggggcg cgctcaagac gcaggaacaa
     2041 cctggtagcg attccaaaag tgccttcgga gccgatgaag aggcccataa ggtcgta
//
LOCUS       DAOTNG010000441         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_41615_length_2082_cov_4.541687, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000441 DAOTNG010000000
VERSION     DAOTNG010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41615_length_2082_cov_4.541687"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="P5179_14235"
     CDS             <1..184
                     /locus_tag="P5179_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96424.1"
                     /translation="EMEPSDIIRVPWDSPCDLRMTWRWRPGVPPGRAGWRILKTDPAF
                     VRMESTVPFRWCFQGW"
     gene            297..1622
                     /locus_tag="P5179_14240"
     CDS             297..1622
                     /locus_tag="P5179_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS96425.1"
                     /translation="MEESLLSRSLCAYLPDRVFDGRRVLPDHAVLVEDQHVQCVLPAR
                     ELTPDTPIAAYPGCTIIPGLIDAHVHFMRWQGPLYLAHGVTTVRDTGNPLNWILARRS
                     EWEKREWPRMFCTGPLIDGPSPVHPVTSRPCTNISDAIRGVREVAAAGVDGIKLYVGL
                     ETGWIPAMVREANAAGLPVSMHALQTGMVLPARAGVQEFFHLDGILHDVWPDHPPGWL
                     DAWGLPGFEQTLDRQKYLADEIAEREIVMTPTLAYWDSQWRGRLRGYKRHPALRDVPR
                     EVINWQVPEEPDREGSARWKRALKAAQRFVALLREREVTVLAGTDTPCGAAPPGLSLW
                     REMRLLVEAGMNTREALTAATSATGKIPGFQRVGTLCREAAADLVVVRGNPLDAIPDP
                     PDIVLVVRNGEEFDPAALKSRAETVDLSDEPWARQFQAHAEHTPATPWE"
     gene            1649..>2082
                     /locus_tag="P5179_14245"
     CDS             1649..>2082
                     /locus_tag="P5179_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96426.1"
                     /translation="MAAITEKSIELTHGAAKLVIDARTGSVLRLEDMRTGVARIDAKK
                     DGRNDGCLFRTMVPAENWWSRVADSSHQKRVRVSQRGGGATVEFPALVAADGAETGVS
                     ARVDIRPGGPDEFVFVLHLENSGSSTVLDTAFPVLGGWHERGT"
BASE COUNT          366 a          656 c          715 g          345 t
ORIGIN      
        1 ggagatggag cccagcgaca tcatccgcgt cccgtgggat agcccctgcg acctgcgaat
       61 gacgtggcgc tggcggcccg gggtgcctcc gggccgcgcg ggctggcgga ttctgaagac
      121 cgatcccgcg ttcgtccgca tggagagcac ggtgccgttc cgctggtgct tccaggggtg
      181 gtaatgctgc cctgcgggca gccggtttgg cgcgaccgat gcctgagcga taggttcaga
      241 gcgatgtaac ccgtatcgcc caacagctca ccgccacagg ggggtgcaca acctgcatgg
      301 aggagagcct tctctcccgc tcgctctgcg cgtacctgcc cgatcgtgtc ttcgatggcc
      361 ggcgcgtcct gccggatcac gcggtgctgg tggaagacca gcacgtccag tgcgtcctgc
      421 cggcgcggga actcaccccg gacacaccaa tcgcggccta ccccggttgc accatcattc
      481 cgggtctcat cgatgcccac gtgcacttca tgcggtggca ggggccgctc tacctcgcgc
      541 acggcgtgac aaccgtccgg gataccggga acccgctgaa ctggatactc gcccgccgat
      601 cggagtggga aaagcgggaa tggccccgca tgttctgcac cggcccgctc attgacggcc
      661 caagcccggt tcatccggtc acctcgcgcc cgtgcacgaa catctctgac gcgatacgcg
      721 gtgttcgcga ggtcgccgcc gccggcgtgg acggcatcaa gctgtacgtc gggctggaga
      781 ccggctggat accggccatg gtgcgcgaag caaatgccgc cggtttgccg gtgagcatgc
      841 atgcgctgca gacgggaatg gtcctccccg cccgggccgg tgtgcaggaa ttcttccatc
      901 tcgacgggat tctccacgac gtctggcccg accacccgcc cggctggctg gacgcatggg
      961 gcctgccggg gttcgaacaa acacttgacc ggcagaaata cctcgccgac gagatcgccg
     1021 agcgggagat agtgatgacg ccgacgctgg cctactggga ctcccagtgg cgcgggcgtc
     1081 tccgggggta caaacggcac cccgcgttgc gtgatgtccc gcgggaagtc atcaactggc
     1141 aggtcccgga agagcccgac agggaaggtt cggcgcgctg gaaacgggca ttgaaggccg
     1201 ctcagcggtt cgttgctctt ctgcgggagc gggaagtgac ggtgcttgcc ggaaccgata
     1261 ccccctgcgg cgcagctccg ccggggctga gcctctggcg cgagatgcgg ctgctggtgg
     1321 aagccggaat gaacacgcgt gaggcactga cggccgcgac ctcggcaacc ggcaagatcc
     1381 ccgggttcca gcgggtgggc actctgtgca gagaggcggc ggcggatctt gtggtggtac
     1441 ggggtaaccc gctggatgcg atacccgacc cgccggacat agtgctggtg gtaagaaatg
     1501 gcgaagagtt cgatccggcc gccctgaaat ctcgagcgga aacggtcgat ctgagcgatg
     1561 aaccgtgggc aaggcagttc caggcacatg cggaacacac ccctgccacg ccgtgggagt
     1621 aaccactgac gggaaaaagg agctaccgat ggcagctatc accgaaaagt cgatcgaact
     1681 cacgcacggc gcggcgaaac tggttattga cgcccgcacg gggtctgtgt tgaggctgga
     1741 agacatgcgc accggtgttg cccgcattga cgcgaagaaa gacggccgga acgacgggtg
     1801 cctgtttcgc actatggttc cggcggagaa ctggtggtcg cgcgtggcgg atagctccca
     1861 tcagaagcgc gtgcgggttt ctcaacgcgg cggcggcgcc accgtcgagt tccccgcact
     1921 ggttgccgcg gacggcgcag aaaccggcgt atctgcgagg gtggatatcc ggcccggcgg
     1981 accagatgag ttcgtgttcg tgctccatct ggagaactcc ggctcctcca cggtgctcga
     2041 caccgcattt cccgttcttg gtggctggca cgagcgggga ac
//
LOCUS       DAOTNG010000442         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_41718_length_2078_cov_5.319328, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000442 DAOTNG010000000
VERSION     DAOTNG010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41718_length_2078_cov_5.319328"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /locus_tag="P5179_14250"
     CDS             <1..>2078
                     /locus_tag="P5179_14250"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HRS96427.1"
                     /translation="HAVTATKDGQVWAVGDEGVILLHEGNAWRRIAAPSSKNLYAVSA
                     RGVSEAWFGGERVLLYWNGSEVETAPVDVRGVVRGLDVFESRTGWAVGLGTVLRLDPH
                     GARRSGEQATRFGFYRQELLPNALGIFGAAFGDCNGDGLDDLFLVSMRDANHLLVNRG
                     AGSFGDETGVRGLMGTVVTIPVVKEMAQFGASWGDYDNDGRLDLAVAGWYGSTYLYRQ
                     NSARRFDDVTDRLGLREGPFSANSVTFADVNQDGLLDLFVCNEHGSNVLWLNNGRGGW
                     TDGTRRWGLTSRGGSKDAVFGDLDNDGDVDLFVCSGSGSNLVYRNDGTRFVDISTTCA
                     AAGERAEHNGATLADLDNDGDLDIVTTTSDGQNRIWRNNGDWRFEDASGIVGFASGPF
                     SYGSAAGDFDNDGFLDLLIAGADGVTFFRNAGDMRFIPVAVEGLTDVKDARAVAVADV
                     DADGDLDVFVASKADFTDGFSDFEQRRSACFFNRLDLKTSISVRCEGVSSNRMAVGAR
                     VRLYRDTGALSHAGELVGMREIMASNGCRSQQSAAAHFGVDPASRYVVRVTFPSGREV
                     EQRNVRAGQRLVVAEMTGPAAVARRVGQTAWRSIWSNTARRHVFPLVVVIPLLVWAFR
                     NASQRLLWGSLVTNMFTVALSVAYALLAIATVSSSWWVGVLVPAVTVSLIAFLVVGLS
                     YWSGPRAADK"
BASE COUNT          383 a          527 c          723 g          445 t
ORIGIN      
        1 gcatgcggta acggccacga aggacgggca ggtctgggcg gtcggcgacg aaggcgtgat
       61 tctgttgcat gaaggaaatg cctggcggcg gatagcggct ccttcatcaa aaaacctcta
      121 cgcagtgtcc gcccggggcg tttccgaggc gtggttcgga ggagaaagag ttctgctcta
      181 ctggaacggt tcagaagtcg agacagcccc cgttgatgtg cgcggagtcg ttcgcgggtt
      241 agacgtattt gaatcgagaa caggctgggc ggtgggactc ggcaccgttc ttcgactcga
      301 tccgcatggt gcgaggaggt ccggagagca ggcaactcgt tttggcttct acagacagga
      361 actgctgccg aacgcgctcg gaatctttgg agccgcattc ggcgactgca acggcgacgg
      421 ccttgacgat ctgttcctcg tgtcgatgcg tgacgcgaac catttgctcg tgaatcgggg
      481 cgctggcagt tttggcgacg agacgggcgt tcgtggtctc atgggcacag tcgtgacgat
      541 tccggtagtg aaggaaatgg cgcagttcgg tgcatcctgg ggggattacg ataacgatgg
      601 ccgcctggac cttgcagttg ctggctggta cggttccacg tatctctacc ggcagaattc
      661 cgcgcgccgc tttgatgacg tgaccgaccg gctggggctg cgggaaggtc ccttttcggc
      721 gaacagcgtc acattcgccg acgtcaacca agacggtctg cttgaccttt ttgtgtgtaa
      781 cgagcacgga agcaacgttc tctggctcaa taacggaagg ggaggatgga ccgacgggac
      841 tcgccgctgg ggattgacct cccgcggggg gagcaaggat gccgtgttcg gtgatctgga
      901 caacgatggg gatgtcgatc ttttcgtctg cagcgggagc ggcagcaatc tggtgtaccg
      961 aaacgacggc acacggtttg tggacatatc aaccacctgt gccgctgccg gcgaaagagc
     1021 ggagcacaac ggagcgacgc tggcggacct cgacaacgac ggcgatctgg atatcgtgac
     1081 gaccacttcg gacggtcaga accggatctg gcggaataat ggcgactgga ggtttgaaga
     1141 cgcttcaggc atcgtgggat ttgcatccgg acccttcagc tacgggagcg ccgcaggtga
     1201 tttcgacaac gacgggtttc ttgatcttct catagccggg gccgacgggg tgacgttttt
     1261 tcgaaacgcg ggggatatgc gtttcattcc cgttgcagtt gagggtttga ccgatgtcaa
     1321 ggacgcgcgt gcggtagcgg tggcggacgt tgacgccgat ggggacctcg acgttttcgt
     1381 agcgagcaaa gccgatttta ccgatgggtt ttcggacttc gagcagagac ggtctgcatg
     1441 cttcttcaac aggcttgacc tgaagacctc gatcagcgtg aggtgcgaag gggtctccag
     1501 taacagaatg gccgtcgggg cgcgcgtgag gctttaccgc gacacggggg ccctgtcaca
     1561 tgccggcgag ttggtgggca tgcgcgaaat catggcttcc aacgggtgtc ggagtcagca
     1621 gagcgcggca gctcacttcg gtgtggaccc tgccagccgg tacgtagtcc gcgtcacatt
     1681 cccttccggt agggaggtgg aacagcgcaa tgtgcgggct ggacaacgcc tggtcgtcgc
     1741 ggaaatgacg ggacctgcgg cggtagctcg ccgtgtcggg cagacagcat ggagaagtat
     1801 ctggagcaac accgcgcgga ggcatgtgtt tccgctggtg gtggtgatac cgttgctggt
     1861 ctgggcgttc agaaacgcaa gtcagcgttt gttgtggggg tcgctggtta cgaacatgtt
     1921 cacggtagcc ctttcagtgg cgtacgccct gcttgcgatt gctacggttt ccagttcgtg
     1981 gtgggtcggt gttctcgttc cggcagtcac tgtgtcgctg atagcttttc tggtggtcgg
     2041 gctgtcgtac tggtcagggc cgcgcgcggc ggacaagg
//
LOCUS       DAOTNG010000443         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_41957_length_2069_cov_2.436941, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000443 DAOTNG010000000
VERSION     DAOTNG010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41957_length_2069_cov_2.436941"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="P5179_14255"
     CDS             complement(<1..901)
                     /locus_tag="P5179_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96428.1"
                     /translation="MTQTHKSGVPTRQVNRFIMCLALLGICVSAANAAHIRVGRLWDE
                     YSNAGNEAWGGTCMVWPGGFWKEDLGGEKYSHASASYRGFLYGVTNFTVPPDNASWEM
                     PSDQPPGYVYPFFVVQRDIRNTPQANSYQLINGGIKLIYKSNPTTITTDGVANNPPEV
                     SGGEDPTLCADAKIISSWRNNMGLETVRTVYAFAHPLHNDYHVWHYTFKNTGKYCCSS
                     RTTAAGNPNAQYSQTLTDFYFSHSMWYNDRSEGQNATNSCCEWSGNSLTDYKGHNAGT
                     YAAEAVTSDVKQFIANAYGISAIV"
     gene            complement(934..>2069)
                     /locus_tag="P5179_14260"
     CDS             complement(934..>2069)
                     /locus_tag="P5179_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS96429.1"
                     /translation="WGSLLTDWDMAASIKKRFGPNGTVAPDSLQTEDVSAKIHFSPRL
                     GISHPISRTTKVFFNYGHFYAVPQNQYVFGMYPNQPGSIEYLGNPDLDMPRTIAYELG
                     FDQELFGVYLLHVAGYYKDATNEINLTIRKALAPTQPYDVIEPFNKQYSDIRGFEFKL
                     SKRIGRFTTGFWSGGVEMVTTTRLGYARISEDVNEVPVAEFRDAPYRARPYSKLNLDL
                     HTPRDFSLMGLPALATGGWSLNWLTSYQRGADAIYDPVSAPGTLLSQAYVKSPNVSYP
                     DWWMSDMRIQKGFTFGKVNCAAFFDVNNVFARKVWTGSMRGDDLTAYMKSLHFPIDDP
                     LIEDTRGNDKIGDTPSYAVLPIRDQWTLFSYPRTFSFGVRVDF"
BASE COUNT          404 a          588 c          619 g          458 t
ORIGIN      
        1 cgacaatcgc ggagataccg tacgcgttgg cgatgaactg cttcacgtcc gacgtgacgg
       61 cttccgcggc atacgttccg gcgttgtgcc ctttgtagtc agtgagtgag ttgccgctcc
      121 actcgcagca ggagttcgtg gcattctggc cttcagagcg gtcgttgtac cacatcgagt
      181 gcgagaagta gaaatccgtg agtgtctggg agtactgcgc gttgggattt cctgccgcag
      241 tcgtccttga tgagcagcag tacttccccg tgttcttgaa cgtgtagtgc cagacgtggt
      301 aatcgttgtg gagcgggtgc gcgaacgcgt acacggtacg caccgtctcc aaccccatgt
      361 tgttccgcca ggaggagatg atcttcgcgt cggcgcacag ggttgggtct tcaccacccg
      421 acacttccgg cgggttgttc gccacgccgt ccgtggtgat ggtcgtcggg ttggatttat
      481 agatcagctt gatgccgccg ttgatgagct gatagctgtt cgcctggggc gtgtttctga
      541 tatcccgctg caccacgaag aagggataca cataccccgg cggttgatcg gacggcattt
      601 cccacgaggc gttatccggc ggaacggtga agttcgttac gccgtagaga aagccccggt
      661 aggacgcaga tgcgtgcgag tacttctcgc ctccgaggtc ctccttccag aaaccaccgg
      721 gccagaccat gcaggtcccg ccccacgcct cgtttccggc gttggagtac tcgtcccaga
      781 gtctcccgac acggatgtga gccgcatttg ccgcactgac gcagattccg agcagcgcca
      841 gacacatgat gaagcggttc acctgccggg tggggactcc tgatttgtgt gtttgagtca
      901 tgtaccatgc ctcctgtaaa cgtgtttccg ctatcagaag tccaccctga caccaaatga
      961 gaacgtgcgt ggatacgaga acagcgtcca ttgatcccga atcggcaaca ctgcatagct
     1021 gggcgtatca ccgatcttgt cgttgcccct cgtgtcctcg atgagcggat catcaatcgg
     1081 gaagtgcagc gacttcatgt acgccgtcag gtcgtccccg cgcatggagc ccgtccagac
     1141 ttttcgcgcg aacacattgt tgacgtcgaa gaatgcggcg cagttgactt tcccgaaggt
     1201 gaagcctttc tgaatccgca tgtccgacat ccaccagtcg ggatacgaca cgtttggtga
     1261 tttgacgtag gcctggctca gaagcgttcc aggggcgctg acgggatcgt aaatggcgtc
     1321 agcaccgcgc tggtaggatg tgagccagtt gagggaccag ccgccagtcg ccagcgccgg
     1381 aagacccatg agcgaaaaat cgcgcggcgt gtgcagatcg agattcaatt tgctgtaagg
     1441 acgcgcgcgg tacggggcat cccggaactc agcaaccggt acttcgttca cgtcttcgct
     1501 gatgcgcgcg tatcccagac gcgtggtcgt caccatctcc accccgccgc tccagaagcc
     1561 tgtggtgaac cggccgatgc gtttggagag cttgaactca aacccgcgaa tgtctgagta
     1621 ctgcttgttg aacggttcga tcacgtcgta cggttgcgtc ggagcgaggg ccttcctgat
     1681 agtgaggttg atctcgttcg ttgcgtcctt gtagtaaccc gcgacgtgca acagatacac
     1741 tccaaacaat tcctgatcga acccgagttc atacgcgatg gtccgcggca tgtccaggtc
     1801 cgggtttccg agatactcga tcgaacccgg ctggttggga tacatgccga acacgtactg
     1861 gttctgcgga acggcgtaga agtggccgta gttgaagaac actttcgtcg tccggctgat
     1921 cgggtgagag ataccgagcc gggggctgaa gtgaatcttc gcgctcacat cttccgtctg
     1981 cagtgaatcg ggcgccacgg ttccattcgg gccgaaacgt ttcttgatgg acgcggccat
     2041 gtcccagtca gtcagcagag aaccccacg
//
LOCUS       DAOTNG010000444         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_42305_length_2056_cov_5.875062, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000444 DAOTNG010000000
VERSION     DAOTNG010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42305_length_2056_cov_5.875062"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="P5179_14265"
     CDS             <1..468
                     /locus_tag="P5179_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS96430.1"
                     /translation="FWDLDRWLGAVREMGITVLLNEHRVIERGNRKMVLAGVTDYSGG
                     RFRADHVSNPGISLTGAPEADVRILLAHQPRTVYGAAQHGFDLQLSGHTHGGQFYPWV
                     FLVPLQQPYVAGLHRHENTWVYVNRGAGYWGPPLRLGAPSEIALIRLIGRADA"
     gene            complement(491..1804)
                     /locus_tag="P5179_14270"
     CDS             complement(491..1804)
                     /locus_tag="P5179_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96431.1"
                     /translation="MQRTFCLLDPRLVERTENLRLVLGCVRKDARNPLLIEDQPWEVR
                     FDNLYPNVHWSREKGLFECWYNPFIVCEATSNTPDEEKRRIPYRPGKREMGVCYAQSR
                     DGIVWEKPALGLIEFEGSTANNLVMRDIHGVGVYYDRFESDPARRYKALTSVGTATSP
                     DGLRWTLRECPDIGAIGDTHNNLFWDERTRRYVGFTRLWKDRQRIVARTESPDFVSWT
                     RAEEVLRASEDSPHRQIYELIVFPCSSGYLGIAAVLDTESDCVDCELAWSPDTIVWSR
                     VAEGFALIPRGEEGSWDRGCIYAAAYPIITEKELLLYYAGGNDTHMSWRKTGLGLARL
                     RPDGFAGWRPVGTPDRGELVTTSIACSAPNLTVSADAEGGSIRAEVVGVEGFSLESCR
                     RVSADVTDCVMTWGEGRTLAPFVGKAITLRFELLNATLYSIGFSG"
BASE COUNT          418 a          663 c          565 g          410 t
ORIGIN      
        1 ttctgggacc ttgaccgttg gctgggagcc gtccgtgaaa tggggatcac ggtcttgctg
       61 aacgagcacc gggtgataga acgaggaaac cgaaagatgg ttctcgccgg ggtcaccgat
      121 tactccggcg ggcgcttccg cgcggaccac gtctccaacc cggggatttc actcaccggg
      181 gctccggagg ctgatgtccg catcctcctc gcccaccaac cgcgcaccgt gtacggagcc
      241 gctcagcacg gttttgatct ccagctttcg ggacatacgc acggaggaca gttctaccca
      301 tgggtgtttc ttgtaccgtt gcagcagccg tacgtggccg ggcttcatcg gcacgagaat
      361 acgtgggtgt acgtgaatcg cggggcagga tactggggcc ccccactgcg gctcggcgcg
      421 ccttccgaaa tcgcactcat ccgcctgatc ggacgcgcgg acgcctgatt tcgcctcatc
      481 cggacgcgtt ctatcccgaa aagccgatcg aataaagggt cgcgttgaga agttcgaagc
      541 ggagcgtgat ggccttgccg acaaaggggg cgagcgtcct cccttcgccc cacgtcataa
      601 cgcagtctgt cacatccgct gaaaccctcc gacacgactc gagcgaaaac ccttccacac
      661 ccactacttc cgcccgaatc gagccacctt cagcatcagc ggagacagtc agattcggtg
      721 ccgaacaggc gatcgaagtc gtaacgagtt cccctctgtc cggagtccca acggggcgcc
      781 atccggcgaa accatccggg cggagacggg caagccccag cccggtcttg cgccagctca
      841 tgtgcgtatc attgccgccc gcgtaataga gaagcagctc cttctccgtt atgatcgggt
      901 atgcggcggc atagatgcat ccgcggtccc acgagccttc ctctccccgc ggaatcaggg
      961 caaaaccttc ggcaacacgg ctccagacta tcgtatcagg gctccacgca agttcgcaat
     1021 ctacgcagtc gctctcagtg tcaagcacgg cagcaatccc gaggtagcca ctcgagcagg
     1081 gaaagacgat caactcgtat atctgccggt gcggtgaatc ctccgatgcc cgaagtactt
     1141 cttccgcccg tgtccacgaa acgaaatccg ggctttccgt tcgagctacg atccgctgcc
     1201 ggtctttcca taaccgggtg aacccgacgt aacgccgggt acgctcatcc cagaacaggt
     1261 tgttgtgagt gtccccaatt gcgccaatat ccggacactc tcgcaacgtc cacctgaggc
     1321 cgtcgggaga cgtcgccgtc ccgaccgagg taagtgcctt atagcgacgc gcaggatcgg
     1381 attcgaacct gtcatagtag acaccaacgc cgtggatgtc gcgcatgacg agattgttcg
     1441 cagttgatcc ttcgaactcg ataagtccga gagccggttt ttcccagaca ataccgtccc
     1501 gactctgtgc gtagcagacc cccatttctc gctttccggg acggtaggga attcggcgct
     1561 tctcttcatc cggcgtgttg ctggtcgcct cgcacacgat gaaggggttg taccagcatt
     1621 cgaacagccc cttttcgcgc gaccagtgca cgtttggata gaggttgtca aaccgcactt
     1681 cccacggctg atcttcgatc agaagagggt ttctcgcgtc cttccgaacg caacccaaga
     1741 ccaggcgaag gttctccgtt cgttccacga gacgtgggtc aagcaagcaa aatgttcgtt
     1801 gcatagcgcc tccgtgaaga ccatcgcaaa cgggcctgat caattccctc tgagctgttc
     1861 accacaacgc atttctgcgc gtcagatcag cttcagctcg acagatgccg gcgccagccc
     1921 gtccgccgaa gcagtgatgc gcacagtgcc ctttttccgg gctgagcggt acgcaatgcg
     1981 tcctactccg tttcgcgttg aaacgctcgc gttgccgtac aggccgaaaa atgtcccgcc
     2041 ccctgccacc gagaac
//
LOCUS       DAOTNG010000445         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_43376_length_2019_cov_4.566191, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000445 DAOTNG010000000
VERSION     DAOTNG010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43376_length_2019_cov_4.566191"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="P5179_14275"
     CDS             <1..1231
                     /locus_tag="P5179_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96432.1"
                     /translation="LAKEIRDIAHSDENLEKKDAWYAHNDLKPRRPLVLIESFPATDE
                     LVTDDILSCRDDWARKQEKHLRTTIYHFRNIRDDDVIEPYLTWNWHVSAGNYGVTSTR
                     HDSDKIQRVDGKVVGSYSWEAPVKDLDADFGKLKPRVFSVDRESSLLERDRLEDVFGD
                     ILGVRQRGGYWWTTGMTIIAIDLIGLENLMLAMYDNPAGLHRLMGFLRDDHIAFAEWL
                     EKEGLLSLNNENDYIGSGSRGYTRELPQKDWKPGGKVRTRDCWVLSESQETVGVGPGQ
                     FEEFIFPYQKDVVERFGLCYYGCCEPVHSRWHVIKKFSNLRSVSVSPWCDEEMMGEVL
                     AGKYVYSRKPNPTLISTDVFDENAIRNDVAKTLRCARKCSVEIVMKDVHTLAGDPMRA
                     ARWVELARQAIDLEWGK"
     gene            complement(1251..>2019)
                     /locus_tag="P5179_14280"
     CDS             complement(1251..>2019)
                     /locus_tag="P5179_14280"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRS96433.1"
                     /translation="GATVTLIANIGVDSFGEASIERFRAEGIRVGAIARDTEAASGVA
                     MIWVSETGQNSIVVALGSNARLTPQHVEDASGVFQNADVLLTQLETPLPTVAFALRTA
                     RSIGVRTILNPAPATVLGPELLADVDWITPNETEAARISGIEVQNVSDAEHAANALRR
                     MGARNVVVTMGEAGALLMTDDESLHVPARRVQSVDTVAAGDAFNGAFAVALARNAPPR
                     EAVEFACAAASLSVTRRGAQPALPTADEVTKFASQLA"
BASE COUNT          461 a          560 c          603 g          395 t
ORIGIN      
        1 tctcgcaaag gaaatccgtg atatcgcgca cagcgatgaa aacctcgaaa agaaagacgc
       61 gtggtacgct cacaacgacc tgaaacctcg acggccgctg gtgttgatag aatcgtttcc
      121 ggcaacggat gaactggtaa cggacgacat attgagttgc cgcgatgact gggcacggaa
      181 acaagagaag caccttcgaa caacgatcta tcacttccgg aacattcgcg acgatgacgt
      241 tattgaacca tacctcacct ggaattggca tgtttccgca ggtaattacg gtgtaacctc
      301 aacgcgtcac gacagcgaca aaattcaacg ggtcgacggt aaggtcgtcg ggagttactc
      361 ctgggaagca ccggtcaagg acttggacgc ggacttcggc aagctcaaac cgcgcgtttt
      421 ctccgtggat cgcgaatcgt cgcttctcga aagggatcgc ctggaggacg tgttcggtga
      481 catcctcggt gtgaggcagc ggggtgggta ctggtggact accggaatga caatcattgc
      541 catcgatctc atcggcctgg agaacctcat gctggcgatg tacgacaacc cggcagggct
      601 gcaccggctc atggggtttc tgcgggatga tcacatcgcg ttcgcggaat ggttggaaaa
      661 agaggggctg ctgtcactca ataacgaaaa cgattacatc ggctccggaa gcagaggata
      721 cacgcgcgaa cttcctcaaa aagactggaa gccgggcggc aaagtacgga ccagggattg
      781 ctgggtgctt tccgagtctc aggaaacggt gggtgtcggc cccggccaat ttgaagagtt
      841 catcttcccg tatcaaaaag acgtcgtgga acgattcggt ctctgctatt atggatgctg
      901 tgaaccggtt cacagccgct ggcatgtgat caagaagttc agcaacctgc ggagcgtgtc
      961 cgtttccccg tggtgtgacg aggaaatgat gggagaagtg cttgcgggaa agtacgtgta
     1021 tagccgcaaa ccgaacccca cgcttatcag cacggacgtg ttcgacgaaa atgcgattag
     1081 gaacgacgtg gcaaaaaccc ttcgctgcgc ccgcaagtgc agtgttgaaa ttgtcatgaa
     1141 ggacgtacac actctcgctg gagatccaat gcgggcagcg cgttgggtcg agcttgcccg
     1201 gcaggcaatt gatctggaat gggggaaatg atcgagccgc agcgtaggga tcaggccaac
     1261 tgactggcga acttggtgac ttcgtcggcc gtgggcaggg ccggctgcgc accgcgccgt
     1321 gtcacagaga gcgacgcggc ggcgcaggcg aattcaacgg cttccctcgg gggtgcgttt
     1381 ctggcaagtg caaccgcaaa cgcgccgttg aacgcatctc ccgcggcgac cgtgtcgacg
     1441 gactgaacgc ggcgggcagg cacgtgcagc gactcatcgt cggtcatcag gagggctccc
     1501 gcctctccca tcgtaaccac cacgttccgg gctcccatcc tgcgcaaagc gttcgccgcg
     1561 tgttcagcgt ctgaaacatt ttgcacttcg attcccgaaa tccgcgctgc ttcggtctca
     1621 ttgggggtaa tccaatccac gtccgcaagc aattccggac ccaggaccgt ggcgggtgcg
     1681 ggattaagga tcgtcctgac gcctattgaa cgggcagtcc gcaaggcgaa tgcgaccgtc
     1741 ggcagaggcg tctccagttg cgtgagcaag acgtcggcat tctggaagac accggacgca
     1801 tcttccacgt gctgcggggt caggcgtgcg ttggaaccaa gagccacaac gatggagttc
     1861 tggcccgtct cactcaccca tatcatcgcc accccggaag cagcctcagt atcacgcgcg
     1921 attgctccca cccgtatccc ctcggctctg aacctttcaa tagaagcttc cccgaacgag
     1981 tctacgccga tattcgcgat gagtgtgacg gtagctcca
//
LOCUS       DAOTNG010000446         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_43407_length_2018_cov_5.124809, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000446 DAOTNG010000000
VERSION     DAOTNG010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43407_length_2018_cov_5.124809"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="P5179_14285"
     CDS             complement(<1..293)
                     /locus_tag="P5179_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96434.1"
                     /translation="MAFSSPQDGVTAELHPWRTIWTHPRQTVAAMTVYAPWRWLWLLI
                     LAGGTSRIVERIALLPSLVELEPEALTLLILVSGALAGALMVFVIARILHWVLR"
     gene            630..1325
                     /gene="grpE"
                     /locus_tag="P5179_14290"
     CDS             630..1325
                     /gene="grpE"
                     /locus_tag="P5179_14290"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRS96435.1"
                     /translation="MKVTIREEKSKDKNQEKGPVTEGAVEVSSSAPGGTETAYPAEET
                     RQEPASKVEEEAEKEALEQQLAGAQREAAENRDKYLRAVAELENFRRRTAREFAEMSL
                     RAGERILTALLDPADNLARALDAARKMEAEQAGEALTSFIGGVEMIYQQLVSILEKEQ
                     VTPMETVGTTFDPNLHDALVAVERPDVPPNTVVEEVQRGYRLGDRVLRHAKVVVSKAP
                     VAEEKPNENENTQ"
     gene            1367..>2018
                     /locus_tag="P5179_14295"
     CDS             1367..>2018
                     /locus_tag="P5179_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRS96436.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNQPVVITNTEGGRTTPSIVAFTG
                     DNERVVGQAAKRQMITNPENTIYSIKRFMGRRHSEVSQEEKLVPYKVTSAPNGDVRVV
                     ARGKEYAPPQISAMILEYLKKTAEDYLGTTVTEAVITVPAYFNDSQRQATKDAGQIAG
                     LDVKRIINEPTAASLAYGLDKKKNEKIAVFDLGGGTFDISILEVGDGVFEVLSTNGD"
BASE COUNT          504 a          543 c          633 g          338 t
ORIGIN      
        1 cgaagcaccc agtgcaaaat ccgggcaatg acaaagacca tgagcgcacc ggccaaggcg
       61 cccgacacga gaatcaggag cgtcagtgcc tccggttcga gttcaaccag ggacggaagg
      121 agtgcgatcc gctccacgat ccgacttgta ccaccggcca gtatgagaag ccacagccat
      181 cgccacggtg cgtacacagt catggcggca accgtctgtc gcgggtgcgt ccagatggtt
      241 cgccacgggt gcagctccgc ggtcacaccg tcctgtggtg aagaaaacgc catcgtacct
      301 cacgtgcctt cgtcggaccc cgcgtaccat ccattccggc cccctgaaga aggtacccac
      361 cgaggcgggg tttcgccgag gggcaacgcc tcgccggaac aatctgcgaa gcaaaggagt
      421 gttgacgaaa tggcggtgta tgccactatg gcattaccca ttgcgccatt acggcataaa
      481 tcaaagaaaa caaccttccg cgaaccgcac ggcacaggta ttgcgtttct ccatgccccg
      541 aaggtttcgg tcagtcatca agcgattgtt cgtcggctga gatctgtggg tcagacagta
      601 acgtccatct tgtcgctcgg gagacgcgtg tgaaggtcac aattcgtgaa gagaagtcga
      661 aggataagaa ccaggaaaag ggccctgtaa ccgagggagc cgtcgaggtg tcgtcctccg
      721 cccccggtgg cacggaaacc gcgtatccgg cagaggagac gcgtcaggaa ccggcttcga
      781 aggttgagga agaggctgaa aaagaagcac tggagcagca gcttgccggg gcgcagcgcg
      841 aagccgcgga gaacagggac aagtacctgc gcgcggtggc ggaactggag aatttccgcc
      901 ggcgcaccgc ccgcgaattt gctgaaatga gcctgcgggc cggggaacgg attctcacgg
      961 cgcttctgga tccggcagac aatctggcac gtgctctgga tgcggcgagg aaaatggagg
     1021 ccgaacaagc gggagaggcg ttgaccagct tcatcggcgg ggtggagatg atctatcagc
     1081 aactggtctc aatccttgag aaggagcagg tcacgccgat ggagaccgtc ggcaccacgt
     1141 ttgatccaaa ccttcacgac gcgcttgttg cggtggaacg gccggatgtt ccaccgaata
     1201 ccgtcgtaga agaagtgcaa cggggatacc ggctgggcga ccgcgtgctg aggcacgcga
     1261 aggtggtcgt cagcaaggca ccggttgcag aggaaaaacc gaacgagaac gaaaacactc
     1321 aataaatctc tgagccgcct gattcggcgg aagaaaggct tgaaccatgg gaaaaatcat
     1381 aggaattgac ctcgggacta cgaacagctg tgttgccgtg atggagggca accagcctgt
     1441 agtcatcacg aataccgaag gcggacgaac aacaccgtcc atcgtcgcgt tcaccgggga
     1501 taacgaacgg gtggtcggac aggcagcaaa gcgtcagatg ataacgaacc cggaaaacac
     1561 gatctactcc atcaagcggt tcatgggacg acggcactcc gaagtatctc aggaggagaa
     1621 gctggtcccc tacaaagtga catcggcgcc aaacggtgat gtccgcgtgg tagcgcgggg
     1681 caaagagtat gcaccgccgc agatttcggc aatgattctt gagtatctga agaagacggc
     1741 ggaagactat ctcggcacca ccgtgacaga ggctgtcatc acggttcccg cgtatttcaa
     1801 tgactcgcag cggcaggcaa ccaaggatgc gggccagatt gccggactgg atgtcaaacg
     1861 gatcatcaat gagccgactg cggcgagcct ggcgtatggc cttgacaaga agaagaacga
     1921 aaagattgct gtgttcgacc tcggtggtgg aacgttcgac atttcgattc tcgaagtggg
     1981 cgacggggtg ttcgaggtgc tctccacgaa tggcgaca
//
LOCUS       DAOTNG010000447         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_43468_length_2016_cov_3.318205, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000447 DAOTNG010000000
VERSION     DAOTNG010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43468_length_2016_cov_3.318205"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="P5179_14300"
     CDS             complement(<1..1293)
                     /locus_tag="P5179_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96437.1"
                     /translation="MCRDGFHIERVAFGATDDATDIGISPARLAFEPGSRLPSKLPAN
                     VLFSKGHQPPVLRNGEMIAQIHPLVEFLNAHLGDNRLEPRTDYTVHNSGSAVAYLFAD
                     TSVPLTSLLAGSGFRINLLNMVKSARAKEPVPVHLGVLPTEPHLEQPGLQAGQEIARI
                     LSGWGCQTVLAILRDEARVNRFLADQSRGKAVILVPLRGKKGEPPPEHSMAWLKALNE
                     RNAAFQLCSTESNLAYSRHGLATVILGKAGGTLFRAEPEAVAGYREAWFVGLDLGLGG
                     KHTGKVVAIVLTDSNGRLIAHWRARKGNDETLSVETLSEGLAWVVNEAEARRPGQHYY
                     LIRDGVRPHNESLELYLRAMAGCEFTLIEYAKSGVPFMHREGTQPEPGTTILPANSRW
                     AALYPCQAPQRGVFTTPAKFAVPINPANHTLPEIASILT"
     gene            complement(1547..1678)
                     /locus_tag="P5179_14305"
     CDS             complement(1547..1678)
                     /locus_tag="P5179_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96438.1"
                     /translation="MRDIARLPADDRSDLFRLTAQAMNVHEAATANDTVPTGGWSEV"
     gene            complement(1671..>2016)
                     /locus_tag="P5179_14310"
     CDS             complement(1671..>2016)
                     /locus_tag="P5179_14310"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HRS96439.1"
                     /translation="GDTALNLLGVSTHVPAQWVYLSDGPAKQYEWEGGKITFKHRTNK
                     ETSRLSPKTALVVQALKRLGKERVDESVVHALRSKLDAKDRARAAREARYATSWVYEV
                     IRQLAEGKAPDA"
BASE COUNT          352 a          597 c          649 g          418 t
ORIGIN      
        1 ggtgaggatt gacgcgattt ccggcagtgt gtggtttgcc gggttgatcg gcacggcgaa
       61 ttttgcaggt gtcgtgaaca cgccgcgttg cggagcctgg cagggataga gcgctgccca
      121 gcgcgaattc gcgggaagga tggtggttcc cggttccggc tgggtgcctt cgcggtgcat
      181 gaatggaacg cccgatttgg cgtactcgat cagagtgaac tcgcagcccg ccatcgcgcg
      241 gaggtagagt tccagtgact cgttgtgggg acggacgccg tcacggatca ggtaatagtg
      301 ctgccccggt cggcgcgctt cagcttcgtt cacaacccat gcgaggccct cggagagcgt
      361 ttccactgag agcgtctcat catttccctt ccgggctctc cagtgagcta tcagcctgcc
      421 gttggagtcg gtcagcacaa tcgcgacgac tttgcccgtg tgcttgcctc caaggccgag
      481 gtcgagcccg acgaaccagg cttcgcggta accggccacc gcctccggct cagcccggaa
      541 gagcgtgccg cctgctttgc caagaatgac cgtcgcgagc ccgtgccgcg aatacgcgag
      601 gttagactcc gtcgagcaca actggaaggc cgcgtttctt tcattgaggg ctttgagcca
      661 ggccatggag tgttcaggcg gcggttcgcc cttcttgccg cgaaggggaa ccaggatgac
      721 ggccttcccg cgcgattggt ccgccaaaaa gcgattcacg cgggcctcat cccggagaat
      781 tgccagcacg gtctgacatc cccagccaga gaggattctg gcgatctcct gtcctgcctg
      841 gaggccgggt tgctcaaggt ggggctcggt gggcagaacg ccgaggtgga cgggcacagg
      901 ctcctttgcg cgggcgcttt tcaccatgtt gaggaggttg attctgaacc ccgatcccgc
      961 caggagggaa gtgaggggaa cggatgtatc ggcgaaaagg tacgcgacgg cggagccaga
     1021 attgtgcaca gtgtagtcgg ttcgaggttc caggcggttg tcgccgagat gggcgttcaa
     1081 gaactcgacg aggggatgga tttgagcaat catttctccg ttgcggagaa cgggtggttg
     1141 atgccccttg ctgaacagca cgttcgccgg cagtttcgaa gggagtctgc ttcccggttc
     1201 gaacgccagg cgcgcgggtg agattccgat gtcggttgcg tcatcggtgg ccccaaatgc
     1261 gactctctcg atatggaatc cgtccctgca cacgacctcg cttcccggag cgcgcaccga
     1321 gggccggcgc gggtcccata cacgcaggtg gtgggttgtc gcgtcaaaca ggtaggggcc
     1381 cggtagttcg aagcaagcgg aaagaactcg cttccactcg tcgtgttcgt cggccccgtt
     1441 caggcggtaa cattcccaag cgacctgttt ttcggctatt gtgtgcctcc cgcggcctct
     1501 gcacgacgag atgcctgcat gcggcctgaa gaactgcaat aaatggtcac acctcggacc
     1561 aaccacccgt tggcacggta tcgttcgcag ttgccgcctc gtggacgttc atcgcctgcg
     1621 ccgtcagacg gaacagatca ctgcggtcgt ccgcgggaag ccgtgcaatg tcacgcatcg
     1681 ggggccttgc cctccgccag ttgccggatc acctcataca cccatgacgt ggcatatcgg
     1741 gcctctctgg cggcgcgtgc ccggtccttg gcatcgagct tcgagcggag cgcgtgcaca
     1801 accgactcgt ccacacgctc ctttccgagc cttttcagcg cttgcaccac aagagcagtt
     1861 ttgggagaga gcctggaggt ctctttgttc gtccggtgtt tgaacgtgat cttgccccct
     1921 tcccattcat actgtttcgc agggccatcg ctgaggtata cccattgcgc aggcacgtgc
     1981 gtggatactc cgagaagatt gagcgcggtg tccccg
//
LOCUS       DAOTNG010000448         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_43486_length_2015_cov_3.986735, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000448 DAOTNG010000000
VERSION     DAOTNG010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43486_length_2015_cov_3.986735"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5179_14315"
     CDS             complement(<1..271)
                     /locus_tag="P5179_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRS96440.1"
                     /translation="MGTEKTTIGKALGQIPGGVFVVTAGKGEKGTGFLASWVQQAAFE
                     PPLISIAVKQGRPIQALIESGEPFAVHVLGKDQKKIAAHFAKGFAP"
     gene            464..1966
                     /gene="zwf"
                     /locus_tag="P5179_14320"
     CDS             464..1966
                     /gene="zwf"
                     /locus_tag="P5179_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRS96441.1"
                     /translation="MKDNVIVIFGASGDLTARKLVPALFNNFRKGRLPERTHIIGFSR
                     TRFTDDAFREKMRDAVQTFAPRDFSSDTWTDFAPLLHYQPGDLARHNDFSALEQLIHA
                     LAGGECNRLYYLATAPEFYALAVTMLGTTGMAAETEKTGYRRVIVEKPFGHDYASSVA
                     LNATLHDVFAEQQVYRIDHYLGKETVQNILVFRFGNAIFEPLWNRRYVDHVQITVAET
                     VGVGHRAAFYETAGVIRDMFQNHLLQLLTLIAMEPPYTYEADALRNEKVKVLQAIRPI
                     DEERFLRDVVLGQYSGYREEKGVSPRSETPTYAAVRLLIDNWRWQGVPFYLRSGKKLA
                     QKASEISVVFRRPPHQLFGDHCEFFTNRLTLCIQPNEGLHLRFSAKVPDAGMVMQAAN
                     MGFSYEKWFAERVIPEAYERLLLDALNGDPSLFARSDEIEYAWKIVDSVHSAWEKNPE
                     YHVQPYEPGTWGPKAADQMLTRTGGHWTHGCGALDEVEEGDTRSLSLRGP"
BASE COUNT          388 a          671 c          580 g          376 t
ORIGIN      
        1 cgggcgcgaa ccccttcgcg aagtgcgcgg cgatcttctt ctggtccttg cccaaaacat
       61 gcaccgcaaa cggctctccg gattcaatca gcgcctgaat cggcctcccc tgtttcaccg
      121 cgatgctgat aagcggcggc tcaaatgctg cctgctggac ccacgacgcc agaaaaccgg
      181 tgcctttctc gccttttcct gcggtcacaa caaagactcc gccggggatc tgccccagcg
      241 ccttgccgat tgtcgtcttt tccgttccca tcgcacgtct ccctgtcact ctgactgtga
      301 ggtgtgaatt cgttcctccc aacgcctgcg cggccgaacg ccggttgtcg taccggctca
      361 gttcactcag gcgccaaacc gtgttacttt taccaaacgc cgcgggatgt gggaaagttc
      421 accgggccga cgcaaccctg cgcctcggag ggtaggtagt gccatgaagg ataacgttat
      481 tgtgatattc ggcgcctcag gcgatctcac cgcccggaag ctcgtcccgg cgctgttcaa
      541 caacttccgc aaagggcgcc tgccggaacg gacgcacatc atcggctttt cccgaacgcg
      601 tttcacggac gacgcatttc gtgaaaagat gagggatgca gtccagacgt tcgcgccgcg
      661 ggatttctcg tccgacacct ggacggactt cgcacccctt ctccattacc agcccggcga
      721 ccttgcccgg cacaacgact tcagcgcgct ggagcagctt atccacgcgc ttgcgggcgg
      781 cgagtgcaac cgcctctact acctcgccac cgcgcccgag ttctacgcgc tcgccgtcac
      841 catgctcggc accaccggca tggcggcgga aaccgagaaa accggctacc ggcgcgtgat
      901 cgtggagaaa ccgttcgggc acgactacgc ctcctcagtc gccctcaacg caactctgca
      961 cgacgtgttt gccgaacaac aggtctaccg catcgaccat tacctcggca aggaaacggt
     1021 gcagaacatc ctcgtctttc ggttcgggaa cgccattttc gaacccctct ggaaccgccg
     1081 gtacgtggat cacgtacaga ttacggttgc ggagaccgtt ggcgtgggcc accgggctgc
     1141 gttttatgag accgccggcg tgatccgcga catgtttcag aaccacctcc tgcaattgct
     1201 caccctgatc gccatggagc cgccgtacac gtacgaggcg gacgcgctcc ggaacgagaa
     1261 ggtgaaggtc ctgcaggcaa tccgcccgat agacgaggaa cggttcctgc gcgatgtcgt
     1321 gctggggcaa tacagcgggt accgcgaaga aaagggtgtc tcgccgcgtt ccgaaacacc
     1381 tacgtatgcc gccgtgcgcc tgctcatcga taactggcgc tggcagggcg tgccgttcta
     1441 cctgcgttcg ggcaagaagc tggcgcagaa ggcgtcagag atcagtgtcg tgttccgtcg
     1501 ccccccgcat cagcttttcg gcgatcactg cgaattcttc acgaaccgcc tcaccctgtg
     1561 catccagcca aacgaaggcc tccacctgcg cttttccgca aaagtgcctg atgccggaat
     1621 ggtgatgcag gcggcgaaca tgggcttcag ctacgagaag tggttcgccg agcgcgtcat
     1681 tcccgaggcg tacgagcgcc tccttctcga cgccctcaat ggcgacccct ccctgttcgc
     1741 ccgcagcgac gaaatcgagt acgcgtggaa aatcgtggat tccgtccact ccgcgtggga
     1801 gaagaacccg gaataccacg tccagccgta cgaacccggc acgtggggcc ccaaagccgc
     1861 cgaccagatg cttacccgca ccggcggcca ctggacccat ggttgcggtg cgttggacga
     1921 agtggaggag ggcgacaccc ggtcactgag cctgcgcggt ccctgagctt gtcgaagggc
     1981 gcgcaggagc ctgtcgaagt gccggggcac cagag
//
LOCUS       DAOTNG010000449         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_43750_length_2007_cov_3.477459, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000449 DAOTNG010000000
VERSION     DAOTNG010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43750_length_2007_cov_3.477459"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2007
                     /locus_tag="P5179_14325"
     CDS             <1..>2007
                     /locus_tag="P5179_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96442.1"
                     /translation="DDVIRPHVSVQIGRHHPERNRVLLNHAQAAGVPITLQIQGDNGD
                     RTDTVPMEEIRTLVDSFPCIVGLEIGEASQRTFTDHGSGPEYSMGRNARYTRDAIRIA
                     GEYGLYFTWQLQRDSFLAIGCSSDNEALFDAICEHRAHVIPMHEMNGEFVKHANHTGA
                     MGMWASGATNAWGVEAQSWYWHDAGYNTPGTYFPGTIEMPGALYAIMFLLGASAGATA
                     YAIEPPRDVWPGGEGAWRFEEWVAPVFRRLILERLIPTREEVLAATPAAYHLPRCRRH
                     RDYRQVLDDLSFDLGEGRLARAAYGVFDRARESEMIPNNPRYGWIPALPAKTPREVLD
                     KFPLVLRPGDMRSTEEAREKLDSIYPPVERGTAWSTVVGPLTLAMNTHENWFVGETAR
                     LKVPPPPVHVHIVREAGELALRWTPNSGDSGYRVWRLRGGVEECVASPGAGVTATILR
                     DAAEGDRYAVSAATPMTEEIEFTLHLHDFLVFSNRESRRSPWIGLDGSVVERPRIGEV
                     PPGAHPDAQDKERLCAACSPIEDLASPVIAQNEPAREAKRAVSEAIIGWKRALEREDV
                     DAVLTFYDPDYREADGRTVESVGVALRSIFWRYLEESAGSVAAEWGSIYAWSHPAVRI
                     VTRGWHVVSPDVIEVDTKTMMWAGSGPEMEPSDIIRVPWDSPCDL"
BASE COUNT          367 a          638 c          675 g          327 t
ORIGIN      
        1 gatgacgtaa tccgccccca cgtgtcggtt cagattggac ggcaccaccc ggaacgcaac
       61 cgggttctcc tcaaccacgc gcaggcggca ggagtgccga tcaccctgca gattcagggg
      121 gataacggcg atcggacgga taccgtcccg atggaagaaa tccgtacgct ggtggattcg
      181 ttcccgtgca ttgtggggct ggagatcgga gaagcaagcc agcgcacctt caccgaccac
      241 ggaagcggcc cggagtactc catgggacgg aacgcccgct acacgcgcga tgcgattcgg
      301 atcgccggcg aatacgggct ctatttcacc tggcagctcc agcgggatag cttccttgcc
      361 atcgggtgtt cgtccgacaa cgaagcgctg ttcgacgcca tctgcgagca ccgggcacac
      421 gtgatcccga tgcacgagat gaacggcgaa ttcgtcaaac acgccaacca cacaggggcg
      481 atggggatgt gggcttccgg cgcgacgaac gcctggggag tggaggcgca gtcgtggtac
      541 tggcacgacg cggggtacaa tactccgggg acgtatttcc ccggcactat cgagatgccg
      601 ggcgcgctgt acgccatcat gttccttctc ggggcaagcg ccggggcgac cgcgtacgcc
      661 attgaaccgc ctcgcgacgt atggcccggt ggagagggcg catggcgatt cgaggaatgg
      721 gttgcaccgg tgttccggcg gctgattctc gagcgcctca ttccgacgcg ggaggaagtc
      781 ctcgcggcca ctcccgcggc ataccatctc ccccggtgcc gccggcaccg cgattaccgg
      841 caggtgctcg atgatctgag cttcgacctc ggcgaggggc gcctcgcacg ggcggcgtac
      901 ggcgtcttcg accgcgcacg cgaatcggaa atgataccca acaacccccg ctacgggtgg
      961 attcccgcgc ttccggcaaa aaccccgcgg gaggttctcg acaaattccc gctcgtgctg
     1021 cgtccgggcg atatgcggtc cacggaagag gcgcgggaga aacttgactc catataccct
     1081 ccggtggagc gaggcaccgc gtggagcacc gtcgtagggc cgctcacgct ggcaatgaac
     1141 acgcacgaga actggttcgt gggagaaacc gccagattga aggtcccccc gccgcccgtg
     1201 cacgtgcata tcgttcgcga ggcgggtgaa ttggccctcc ggtggacgcc gaacagcggg
     1261 gattccggat accgcgtctg gcggcttcgc ggcggggttg aggagtgcgt tgcctcaccg
     1321 ggcgccggtg ttacggccac aatcctgcgg gacgcggctg aaggagaccg gtacgccgta
     1381 tccgccgcga caccgatgac cgaggagatc gagttcacgc tccatctcca cgactttctc
     1441 gtgttctcga accgggaaag ccgccgcagc ccgtggatcg ggctggatgg ctccgtcgtg
     1501 gagcgtcccc gaatcggcga ggtgcccccg ggcgcccatc cggacgcgca ggacaaggaa
     1561 cgcctctgcg ccgcctgctc gcccatcgag gatcttgcct ctcccgtgat cgcgcagaac
     1621 gaacccgccc gcgaggcgaa gcgcgccgtg tcggaggcga tcatcgggtg gaaacgggcg
     1681 ctggagcgcg aagatgtgga tgccgttctc accttctacg acccggacta tcgagaagcg
     1741 gacggccgca cggtggagtc cgtcggcgtt gcattgcgct cgattttctg gcgctacctc
     1801 gaggaaagcg ccggcagcgt ggcggcggaa tggggttcca tctacgcgtg gagccatccg
     1861 gcggtgcgca ttgtcacaag gggctggcac gtcgtttcac cggacgtgat cgaggtagac
     1921 acgaaaacca tgatgtgggc cggcagcggc ccggagatgg agcccagcga catcatccgc
     1981 gtcccgtggg atagcccctg cgacctg
//
LOCUS       DAOTNG010000450         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_44272_length_1990_cov_3.510078, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000450 DAOTNG010000000
VERSION     DAOTNG010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44272_length_1990_cov_3.510078"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5179_14330"
     CDS             complement(<1..217)
                     /locus_tag="P5179_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96443.1"
                     /translation="MGFSVRQPNPSGCSVLAFTAGWLPLTHLSASIYVRGVPSPDGEI
                     PTMPPLVCPHWLGYSLIIPWRKWVHNPE"
     gene            269..>1990
                     /locus_tag="P5179_14335"
     CDS             269..>1990
                     /locus_tag="P5179_14335"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS96444.1"
                     /translation="MFRAAFAICVFFAASAHAAQGFVIAREGRPVAKIVTAPDIDRPF
                     DQGVRNQAVRDLIRVVAIMSGAPLPAVQDTLAFNDGPQIHIGQTEFVKRERLLPAGLP
                     VGGYRILTTSERGQPRLVIAGATVSGTMNGVYDLLTTELGVVWGIDSPLFEEVPRRST
                     VALPAIDRVGRSGLPFCMVTGPSLAWQLRNRLSDRGVEYPYSRHGHNMMAFFRISQYR
                     DHPEYYPLVNGQRKIPRQDEDTRVQLCFTNPDVIRITIEKLRAYFDANPTILGYSLCA
                     ADDDEYCTCESCARLDQDAPEFRNRRIHTDSYFHYINEVAKALKQTHPDRYISAYAYD
                     ATEPPPRHIPRLPDNVVVFLTQDATQHFDPVYRQEDEAILVAWKRVAAHVCLYEYPSL
                     GWILPRNYTSLVAQRMPYLHCAGIDGLYAEGHPHPAHLAPALYLTAKLAWEPTANADS
                     VMDLWYRAMFRESAPQMQRFYVTLDSAWTHAERRGFWFAGSNRMWEEFAAYPPLYRET
                     AWHHLNAALRTANNDVTRQRVAYIRAGHEPAYRVARAFEWAHALTPESTEEEIMRVLD
                     EFEMFMTT"
BASE COUNT          390 a          673 c          592 g          335 t
ORIGIN      
        1 tttccgggtt gtgaacccat ttccgccacg gaatgatcag cgaataaccg agccagtggg
       61 gacagacaag tggcggcatg gtaggaatct ctccatcggg tgaaggcacc cccctcacgt
      121 agatactcgc agacagatgc gtcaacggca accaccccgc cgtgaaagcc agcacggaac
      181 accctgacgg gttgggctgg cgtaccgaaa aacccatctt tcccctatcg ttgttcgacg
      241 atgcagcccg atgaaaggag gacgattcat gttccgagcc gcgttcgcca tctgcgtgtt
      301 cttcgctgcc agtgcccatg ctgcgcaggg cttcgtcatt gcccgggagg gcaggccggt
      361 ggcgaagatc gtcaccgcgc ccgacatcga ccgcccgttc gatcaggggg tacgcaatca
      421 agccgtgcgc gacctgatcc gcgtggtggc aatcatgagc ggcgcgcccc tgcctgcggt
      481 gcaggatacc cttgcgttca acgacggtcc gcagatccac atcgggcaga cggagttcgt
      541 gaagcgcgaa cgcctgctgc ccgccggatt gcccgttggg gggtaccgca tcctgacgac
      601 ttccgaacgc ggacagccac ggcttgtcat cgccggggcg acggtatcgg gcacgatgaa
      661 cggcgtatac gacctgctca ccaccgaact cggcgtcgtg tggggcattg acagcccgtt
      721 gttcgaggaa gttccccggc gttccaccgt ggccctgccg gcaatcgacc gcgtcgggcg
      781 ctccggcctg cccttctgca tggtcacggg ccccagcctg gcgtggcagc tacgcaaccg
      841 gctgagcgac cgcggcgtgg agtatccgta ttcccggcac gggcacaaca tgatggcttt
      901 tttccgcatc agtcagtacc gcgatcaccc cgagtactac cccctcgtga acggtcagcg
      961 gaaaatcccg cggcaggatg aggacacgcg cgtgcagctc tgcttcacga accccgatgt
     1021 catccgtatc accatcgaga agctgcgggc ctatttcgac gcgaatccca ctatcctcgg
     1081 ctacagcctc tgcgccgcgg acgacgacga atactgcacc tgcgaaagct gcgcgcggct
     1141 tgaccaagat gcgcccgaat tccgcaaccg aagaatccac accgacagct atttccacta
     1201 tatcaacgag gtcgcgaagg ctctcaaaca gacgcatccc gaccgctata tcagcgccta
     1261 cgcgtacgat gccacggaac cgcccccgcg ccacataccc cgcctgccgg acaacgtggt
     1321 ggtctttctc acacaggacg ccacccagca ctttgacccg gtgtaccggc aggaggacga
     1381 agccatcctg gtcgcgtgga agcgcgtggc ggcgcacgtc tgcctgtatg aatacccgag
     1441 cctcgggtgg attctgccgc ggaactacac ctcgctcgtg gcgcaacgaa tgccgtacct
     1501 gcactgcgcc ggcatagacg ggctgtatgc cgagggacac ccccatcccg cgcacctcgc
     1561 gccggcgctc taccttaccg cgaagctggc atgggagccg accgccaacg ccgacagcgt
     1621 tatggacctc tggtaccgcg ccatgttccg tgaatccgcg ccccagatgc aacggttcta
     1681 cgtgacgctg gacagcgcat ggacacatgc cgagcgccgg gggttctggt ttgccggctc
     1741 caaccggatg tgggaggaat tcgccgcgta cccgccgctc taccgggaaa ccgcgtggca
     1801 ccacctgaac gccgcgctgc gcaccgcgaa caacgatgtc acccggcaga gagtggcgta
     1861 catccgcgcc gggcacgaac cggcctaccg cgtggcccgc gcattcgagt gggcgcatgc
     1921 gctgactccg gaaagcaccg aagaagagat catgcgcgtt cttgacgagt tcgaaatgtt
     1981 catgacgacg
//
LOCUS       DAOTNG010000451         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_44637_length_1978_cov_3.313573, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000451 DAOTNG010000000
VERSION     DAOTNG010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44637_length_1978_cov_3.313573"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="P5179_14340"
     CDS             complement(<1..1196)
                     /locus_tag="P5179_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRS96445.1"
                     /translation="MEQETGALGIIVEPDVPVRMRDGITLRANVFRPNSSGRFPALVQ
                     RTPYGKAQGGFEDFVRAGYVVVTQDTRGRYASEGEFKVFSEENTGDAEDGYDTVEWAA
                     VQPWCDGKVGTFGASYNAWMQYQLARLRPPHLVAMSAVSIPTELTNLDWPGAFKPARR
                     VKWWVTTIAPDLRRRKGLPPPHTPAEAAKIWDELEQGRMLALLPWINVARYLPAPLAD
                     QVAGWLRHPDRRPWRFEEAHRDIEVPNLDFTGWFDHCCSISQFIGMRANARTEKARKH
                     TKVVIGPWNHCNPGRRKQADFDFGPDAEVNVQQMQIRWFDYWLKGIDNGVPREPPVRY
                     FVMGSGRWKSAETWPPPNLGRMTLYLAREGDADLPGGFGALGHEPPKNGPPDTYTHDP
                     FDPVPTL"
     gene            complement(1314..1793)
                     /locus_tag="P5179_14345"
     CDS             complement(1314..1793)
                     /locus_tag="P5179_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96446.1"
                     /translation="MRGPHAQPDFPQHGRYSQSGYRQNIGNVDYGHVHDVLFEDIRVE
                     MDPVYQRPRIQRDDAEVFPVDPHSTWLPTLMACSITQHHEYSEGRIERGHISDVTFRN
                     IHVSSDRMPPSSFRGYDAHHRVANVTIDGLYLNGIRMKTLEEAAVTIGDFAENIALK"
BASE COUNT          376 a          610 c          596 g          396 t
ORIGIN      
        1 agagtcggga cgggatcaaa cgggtcgtgg gtgtacgtgt cgggcggtcc gtttttcggg
       61 ggttcatgtc ctaaggcccc gaagccgccg ggtaggtcgg catctccctc gcgtgcaagg
      121 tacagggtca tccggccgag gttcggcggt ggccacgtct cagccgattt ccaccttccc
      181 gatcccatga caaagtagcg caccggaggc tcccggggca caccattatc gatccctttg
      241 agccaataat cgaaccagcg tatctgcatt tgttgcacat tcacttcggc atccgggccg
      301 aaatcgaagt ccgcttgttt tcggcggccc gggttgcagt gattccaggg cccgatgacg
      361 acttttgtat gcttgcgcgc cttctcggtg cgggcattcg cccgcattcc gatgaactgg
      421 cttatgctgc agcagtgatc gaaccagccg gtgaagtcga ggttaggcac ttcaatgtcc
      481 ctgtgagcct cctcgaatct ccacgggcgt cggtcaggat gccgcaacca tccggccacc
      541 tggtcggcga gcggtgcggg gagataccgg gcaacgttga tccagggcaa aagggcgagc
      601 attctgccct gttcgagttc gtcccaaatc ttcgcagcct ccgccggcgt gtgaggcgga
      661 ggaaggccct ttcgacgccg gagatcagga gcgatggtgg tcacccacca tttgacgcga
      721 cgcgccggct tgaacgcgcc gggccagtcc aagttggtca gttcggtcgg aatggaaacg
      781 gcgctcattg ccaccagatg tggcggccgg agccgggcaa gctggtactg catccaggcg
      841 ttgtaggatg cgccgaatgt gcccaccttc ccgtcgcacc agggctgaac agcagcccat
      901 tccacggtat cgtaaccgtc ctccgcgtcc cccgtgttct cctcggagaa caccttgaat
      961 tcgccctcag acgcgtagcg accgcgggta tcctgggtga cgaccacgta ccccgcacgg
     1021 acaaagtcct cgaaccctcc ctgagccttg ccgtacggcg tcctctgcac aagcgcgggg
     1081 aaacggcctg atgaattcgg acggaacacg ttggcgcgca acgtgatgcc gtcccgcatc
     1141 cgcacgggga catcaggttc gacgataatg cccaacgcac cggtctcttg ctccatggcg
     1201 tggtctcctc cagatgctaa ttcggatcca ggcggcgcaa ggtgtccgcg acagtaatcc
     1261 gctgatggcg tgttcgcagc cagaaggtcg aacggggccg cgattgccct cggctacttg
     1321 agcgcgatgt tttccgcgaa gtccccgata gtcacggcag cttcttcgag cgtcttcatg
     1381 cggataccgt ttaggtacag accatcgatc gtcacgtttg ccacccggtg gtgcgcatcg
     1441 tacccccgga acgaggatgg cggcatgcgg tcggacgaca cgtggatgtt gcggaacgta
     1501 acgtcggaaa tatgcccgcg ctcaattctg ccttcgctgt attcgtggtg ctgcgtgatg
     1561 ctgcaagcca tcagcgtcgg caaccatgtg gagtgcgggt ccaccgggaa cacctccgca
     1621 tcgtctctct gaatgcgcgg ccgctggtag accgggtcca tctccacccg gatatcctcg
     1681 aacagaacat cgtgaacatg gccgtagtcc acattgccga tgttctggcg atatccagac
     1741 tgcgaataac gtccatgttg cggaaagtca ggttgcgcat gtggtccgcg caacactccg
     1801 cgccgatctc caggcagtgg tcccaatcgc accagaccac gcagttctcc accagaatgc
     1861 cttcgcacac cggctgaaag cccaggtgcc ctttgaagca gatggaatcg tcgaacgtgc
     1921 ggacgaaaca gtcggtcaca tgacagcggc ggcagttatg gaaatcgatg ccgtcggt
//
LOCUS       DAOTNG010000452         1976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_44697_length_1976_cov_4.019781, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000452 DAOTNG010000000
VERSION     DAOTNG010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44697_length_1976_cov_4.019781"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="P5179_14350"
     CDS             <1..1222
                     /locus_tag="P5179_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRS96447.1"
                     /translation="PIEFVFDELMDSASVASSLSVVPAIPFHCRWEATRLILGTDTPM
                     DSNTTYRVTICAAACNRWGMPLGADASLEFTTGTEVRCARFRGIWPHHRSVEMQATDF
                     IRVDYSRGVEPASVADAVRVDPPVAGRWQLGDTTASFIPLNFWKAGRYRLTVFPTAHD
                     GSGQNWDVNGDGSVGAGDTLISEFVVAGGVEKRYVFRNLSVILNAQRPDSTFIITIPD
                     RLDSSLVGRIELTPSSVDSADEVWLRVNDGDTLRLPADAPRPWAYRFDARLFVRGENR
                     ITVGINSSSNGVQLMRYGVWVSFTQLPVAVEQSQSVLPVRFELEHPRPNPANPATTIG
                     WVQPEACLVNVEVYDVLGRRIRILTDRHFSAGRHTVVWDGRDDSGRAVGSSVYLVKAR
                     AGNAVQCHKVVISR"
     gene            1387..>1976
                     /locus_tag="P5179_14355"
     CDS             1387..>1976
                     /locus_tag="P5179_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96448.1"
                     /translation="MNTNRARSGMLVFALAVALSGCDVPKTLTPSVELSTSVPLMDER
                     IEIGEEVAKVDSVQTVGSNPARFVISLNRDRVDLTDGGRIGSDQLRVDDQTPQSITAE
                     MGLISVADVPPTTTPPVVLADLVPDIASMPPGNVPLAIPAYHLPTVKRAVDFSNFQQA
                     TFSSSSDQTLNRVVISLANDGPLPMGPVTIYVSTSG"
BASE COUNT          378 a          560 c          608 g          430 t
ORIGIN      
        1 cccgattgaa ttcgtgttcg atgagctcat ggactccgcc tccgtggctt cgtctctttc
       61 tgttgtgccg gcgatcccgt ttcattgtcg gtgggaggcc acgaggctga tcctcggtac
      121 ggataccccg atggattcga acacgacgta tcgggttacc atttgcgctg ccgcgtgcaa
      181 ccggtggggg atgcccctgg gagcagacgc gtcactggaa ttcacgacag ggacagaagt
      241 ccgttgcgcg cggtttcgcg gtatatggcc gcaccaccgg tcagtggaga tgcaggccac
      301 cgatttcata agggttgatt actcccgcgg agtcgagccc gcaagcgtgg cggacgccgt
      361 acgcgtggat ccgccggtgg cgggccgttg gcagcttggc gatacgactg ccagtttcat
      421 tccgctcaat ttctggaagg ctggaagata ccgtctgacg gtctttccaa ccgcgcacga
      481 tggatcgggg cagaactggg atgtgaacgg cgatggctcg gtcggggcgg gcgacacact
      541 tatcagtgag ttcgttgtgg ctggcggtgt ggagaaacga tacgtgttcc gaaatctgtc
      601 tgtcatcctg aatgcccagc ggcctgattc gacgttcatc ataaccattc ccgacagact
      661 cgatagctcg ctggtaggga gaatcgagct tacccccagt tccgtggatt ccgcggacga
      721 ggtctggctc agggtgaacg acggggatac gttgagactt ccggcggacg cgccccgccc
      781 gtgggcgtat cggttcgatg cgcggttgtt cgtacggggt gagaacagga ttaccgtggg
      841 tatcaattct tcttcaaatg gcgttcagct catgagatat ggtgtctggg tgtccttcac
      901 tcagttgcct gttgccgtag aacaatcgca atcagttctt ccggttcgat tcgaactgga
      961 acacccgcgt ccaaaccctg cgaaccccgc cacaacaata ggctgggtgc aaccggaggc
     1021 gtgtcttgtg aatgtggagg tctacgatgt ccttggccgt cggattcgca tcctcacgga
     1081 ccgccacttc tccgccggac ggcacacggt ggtctgggat ggacgagacg actcaggacg
     1141 ggcagtgggt tcctctgtct atctcgtgaa agcgcgcgcc gggaatgccg tgcaatgcca
     1201 caaagtggtc atctcccgct agaagccttc aaggtgtcca tcatctctct gccagacagc
     1261 ctcgtctgca cgcctgacgg ggaaccgacg agtcgcgaga ttttgacgag cgtcaggaac
     1321 gagatacatt tggggtaatc aggggtttgg ccgcggacag aatccagtaa cctgaaggag
     1381 cgcattatga acaccaaccg ggcgcgatcc gggatgctcg tgttcgccct cgcagtcgcg
     1441 ctgtcgggat gcgacgtccc gaaaacgctc accccttcgg tggagctttc cacgagcgta
     1501 ccgctcatgg acgagcgcat cgaaattggc gaagaggtcg ccaaggttga ttcggtgcaa
     1561 accgttggct cgaaccctgc ccggttcgtc atcagcttga accgcgaccg ggtggatctg
     1621 actgacggtg gccgcatcgg aagtgatcaa ttgcgggttg atgaccagac gccgcagtcc
     1681 ataacggccg aaatggggct gatcagcgtt gcggatgtcc cgccgacaac caccccgccg
     1741 gtagtgctgg cggatttggt cccggatatt gcgtcgatgc ccccgggcaa cgtccccctt
     1801 gccatcccgg cttatcatct tccaacagta aaaagggcgg tggatttttc gaatttccag
     1861 caggccactt tctccagttc gtctgaccag acgctcaacc gagtggtgat ttcgcttgcc
     1921 aacgacgggc ctttgcccat ggggcctgta accatctacg tgagcacgtc tggcga
//
LOCUS       DAOTNG010000453         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_46103_length_1932_cov_3.985615, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000453 DAOTNG010000000
VERSION     DAOTNG010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46103_length_1932_cov_3.985615"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1726
                     /locus_tag="P5179_14360"
     CDS             278..1726
                     /locus_tag="P5179_14360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS96449.1"
                     /translation="MPFPHLLRDLKEFFRYRPSVLPGELTPADRQDSLRKLIMAWCLG
                     TVFFEGISGAPLVGMFRSLGATPFWIGLLVAIPSLATLAQPIGSYFVARTGSRKRMLI
                     RTAIPGRLIWLVIPTLGFFFPHNYFVIGVMFTLVLFSRLTIAFASPAWFSWVSDLVPE
                     DERGTFWGSRQMWARIFGIAAAITLGWFLGKEPPYYKFIVYFCLMGFIGWLEIFIHRG
                     VMGIRIPVPEQPPTLAALVLRPLRDRRFLPIIVFTFSINFATQIGGGMFSLMMLEEIK
                     LSYLEISVFQVGILGLVTVLSSRYVGRVIDNLREGARLVFYFGALVTSLQALPWAAIP
                     ERAILPVAGTVILGGVGWAAYHIALTGLLMGYSPRDARATYSAMYAVISGFGSAFGSV
                     LGGTIAEALQGMSYTWGPFILTPLRTLYLISFGGRILALFLLPLIREPASVPLGVYVR
                     QLLSMNPFDRDTYVYIKRKFSPPVRNNGSEQE"
BASE COUNT          315 a          595 c          551 g          471 t
ORIGIN      
        1 ggcttgcgaa catggtgaag aaaatcggcg ggcaggtgct gctcatctac cgggaagaga
       61 ccggtcacac caccagctac gaagatggcc gggcgatcat cgagttcgta ctggatacgc
      121 gcacggcttc cgcctgattc tcgtgggttc tgctccacaa gagggaccgg aatagccact
      181 ttgtccccgc catcccgttt attaaacttt gaatagtcca tcgttccagg tttcttccac
      241 ccgcccaccc agggtaaccg caggctgaag ttcgaccttg ccgtttccac acctcctccg
      301 tgacctcaag gaattcttcc gttaccggcc ctccgttctt ccgggtgagt tgacgccggc
      361 tgacaggcag gattcgctgc gcaagctcat catggcgtgg tgtctgggaa cggtgttctt
      421 cgagggcatc agcggcgcgc cgcttgtcgg gatgttccgc agcctcggtg cgacgccgtt
      481 ctggattggt ctgctcgtcg ccattccgtc cctcgcgact cttgcgcaac ccatcggttc
      541 gtattttgtg gcgcgcaccg gttcccggaa gcgtatgctc atccgcactg ccatccccgg
      601 ccgccttatc tggcttgtca tcccgacact cggcttcttt tttccccaca attacttcgt
      661 catcggggtg atgttcaccc tggtgctgtt ctcgcggctt acgattgcat tcgcctcgcc
      721 ggcctggttt tcatgggtga gcgaccttgt gcccgaggac gagcgtggaa cgttctgggg
      781 aagccgccag atgtgggcac gcattttcgg catcgccgcg gcgatcacgc ttggatggtt
      841 cctcgggaaa gagccaccgt attacaagtt catcgtctac ttctgcctca tgggattcat
      901 cggctggctg gagatcttca ttcaccgcgg ggtgatggga atccggatac cggttcccga
      961 gcaaccaccg actctggcag cgcttgttct ccgcccgctc agggaccgtc gctttcttcc
     1021 catcatcgtg ttcacctttt ccatcaactt cgccacgcag atcggcggcg gaatgttcag
     1081 cctcatgatg ctggaggaga tcaaactatc ctacctcgag atatccgtgt tccaggtggg
     1141 tatcctggga ctggtgacgg tgctcagttc gcgttatgtc gggcgggtta ttgacaacct
     1201 tcgcgaggga gcgaggctcg tgttctattt cggagcgctg gtcacatcgc tgcaggcgct
     1261 tccctgggcg gccatccccg agcgcgcgat tctcccggtt gcaggaacgg tgattctggg
     1321 aggtgtcggc tgggcggcgt accatatcgc gcttaccggg ctgctcatgg ggtattcgcc
     1381 gcgggatgcg cgggcgacgt acagcgcgat gtatgcggtc atttcagggt tcggaagcgc
     1441 gttcggctcg gttctcgggg gaaccatcgc ggaagcactg cagggaatgt cgtacacctg
     1501 ggggccattc attctgacac ccctccgaac gctctacctc atctccttcg gcggacgcat
     1561 tctggcgctg ttcctgttgc ccctgatacg tgagcccgcc tcggttcctc tgggcgtgta
     1621 cgtccggcag cttctctcta tgaacccctt cgaccgcgat acgtacgtct acatcaaacg
     1681 gaaattcagt cctcccgtcc ggaacaacgg atcggagcag gagtaagctt ctctccggtt
     1741 ttcccttcag tcgcaatcct ccttgccttg tcagtgggaa cgcagtcctg ccatatttct
     1801 ttgcgcacac aaagcaggcc attccctttc agaagcggtc ctctcgatgg acaatgctcc
     1861 tcgcgagcgt acgtccggcg acggacgcat gccgtggtat tacggcgcgc ggttgtatgt
     1921 gtacatcctg cc
//
LOCUS       DAOTNG010000454         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_47629_length_1886_cov_5.318405, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000454 DAOTNG010000000
VERSION     DAOTNG010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47629_length_1886_cov_5.318405"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1747)
                     /locus_tag="P5179_14365"
     CDS             complement(338..1747)
                     /locus_tag="P5179_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRS96450.1"
                     /translation="MAQPVPVALGAVLQPRQNAVIQRSGTVAHVAVQIEATEKGRLFA
                     RVKRDGSTLSKFDWRTIGQSNGTLATGVIESLPVGGEYTVEFELRNFRGVVLGKASVK
                     GLLVGDLWILGGQSNMDGYGKLVGTETPSKHVHAFYFDDRWDLAADPLCWYNEAVDSV
                     HWFTADPVERAKAAKNDRIFRQQGAGLAVSFGKTMVKRTEVPVGLIVCSHGGTSMEQW
                     DPGKLELGGASLYGSMIRRVQAVGGKVAGMLWYQGESDANKDALPHFHSRMVNFVKAI
                     RKDLGAPKMPFLYVQIGPFFTGGEIAPYWNGLQADQLAQEKELAPALMTTAIDLGLDD
                     AIHISTQGQRRLGKRMAHLAEILAHGRTSLRKGPRPANIRFSDKNRTILRVHYSDVNS
                     ELYPKRDVRGFWARFEGKDLAILKQEVDLEKPDTVVLTFAEPVPKKAELWYGIGVNPA
                     VNLVDEADMAAPMFGPVVI"
BASE COUNT          345 a          618 c          562 g          361 t
ORIGIN      
        1 acaccgtccg ccgaccgtcc ggcggacgcc actcgatgac cgctgttcgg gcgccacatg
       61 ccggccgtcg agtggctcag gagcgcacgc gagggagatg tatcgagacg caccggcacg
      121 ttacatacga gccgacggaa ggtgaaccgc cgggccgggc tcctcgatac agcgctcgga
      181 ctgcgtcctc ccgccgctcg gagaccggcc tggggccacc ccctgtccgc agagtccacc
      241 agactgcgcc ctcgaaccga agtagaagcg ccccgagcgc actcatgtgc acccggggcg
      301 ctatcgttcg agacgcgcga aacgcgttgc ttcggaacta gatcaccacc ggcccgaaca
      361 tcggcgcggc catatcggcc tcgtccacca gattgaccgc cggattcacg ccgataccgt
      421 accagagctc cgcctttttc ggcaccggct cggcgaacgt cagcaccacg gtgtccggct
      481 tctcaagatc aacttcctgt ttcaggatgg cgaggtcctt gccctcaaat ctcgcccaga
      541 agccgcgcac gtcgcgttta gggtagagtt ccgagttgac gtcggaatag tgcacgcgaa
      601 ggatcgtgcg gttcttgtcc gagaagcgga tgtttgccgg gcgtggacct ttgcgaagcg
      661 aagtacgccc gtgtgcgagg atttccgcga ggtgggccat gcgcttcccg agacgtcgct
      721 ggccctgcgt gctgatgtgg atcgcgtcgt ccagaccgag atctatcgcc gtagtcatca
      781 gcgccggcgc aagctccttc tcctgcgcga gctgatccgc ctgcagaccg ttccagtacg
      841 gcgcgatttc cccaccggtg aagaacggac cgatctggac atacaggaac ggcatttttg
      901 gcgcgccgag atccttccga atcgccttga cgaagttcac catgcggctg tggaagtgcg
      961 ggagagcatc cttgttggcg tcggattctc cctgatacca gagcatgccg gcgactttgc
     1021 cgcccacggc ctgcacgcgc cggatcatgg atccgtaaag ggaagcgccg cccagctcca
     1081 gcttgccagg gtcccattgc tccatgctgg ttccgccgtg ggagcacacg atcaacccga
     1141 ccggcacctc cgtgcgcttc accatggttt tgccgaagga gacagccagt cccgctccct
     1201 gttgccggaa gatgcggtca ttctttgcgg ccttcgcgcg ctcaacgggg tccgcggtga
     1261 accagtgcac cgagtccacg gcctcgttgt accagcagag agggtctgcg gcaagatccc
     1321 agcggtcgtc gaagtagaac gcgtggacgt gcttcgaggg cgtttccgtg ccgacgagct
     1381 ttccgtatcc gtccatgttc gactggccgc caagtatcca caggtcgccg accagcagcc
     1441 ctttcaccga tgcttttccc agcaccaccc cgcggaaatt gcgcagttcg aactcaacgg
     1501 tgtactcccc gccgacaggc aggctttcaa tcacgccggt tgccagtgtt ccgttgctct
     1561 ggccgatggt ccgccagtcg aacttcgaaa gtgtgctccc gtcgcgtttc acgcgggcaa
     1621 acaggcggcc tttctccgtc gcttcaatct gaacggcgac atgcgccacg gtgcccgaac
     1681 gttgaattac tgcgttttgt cggggttgca acacggcccc gagagcaacg ggaactggtt
     1741 gagccatcga atcctgcctc ctttgccact gcggcgatct gatgtacgtt cacgtgcggt
     1801 tatcagagat aaaagaacgc cacgcaagac ccgacgcgca atacgggagt gtggcgacag
     1861 ggatacgcgt caccattttt catgcg
//
LOCUS       DAOTNG010000455         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_48415_length_1865_cov_4.027624, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000455 DAOTNG010000000
VERSION     DAOTNG010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48415_length_1865_cov_4.027624"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="P5179_14370"
     CDS             <1..1374
                     /locus_tag="P5179_14370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRS96451.1"
                     /translation="VLLERCTHILVGNEVAPLDDARQAASVGNIEALSAQAFRTIAVA
                     YRNLEGEGVIPAEEGAPVDESLEADLVYVGIVGILDPPRPEAAKAVVESHRAGVRVIM
                     ITGDHPKTATRIASDLGIVGKDATALTGMEIDKLDEAALREAVRTTSVYARVAPEHKL
                     QIVDALQAGGNVVAMTGDGVNDAPALKRAEIGVAMGVTGTDVTKEAGNMVLADDNFAT
                     IVAAVHEGRVIFDNIRKFLRYLLSSNMGEVLAVFFGVVLSTVIGLTSGDGGIVLPLLA
                     TQLLWINLVTDAAPAMALGVDPETEDVMARPPRKLTDRVIDGRMWTTVLIGGSVMAAM
                     TLLTLDILLPGGLFEGKGSLDTARTAAFTVLVFTQLFNVFNSRSEHASAFHRLCANRW
                     LLGAVLLGVVLQVAVVHIPFMNTAFGTTPLTREQWILCVVMASAVLWVEEARKLVWRT
                     TNLKGKK"
     gene            1371..>1865
                     /locus_tag="P5179_14375"
     CDS             1371..>1865
                     /locus_tag="P5179_14375"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS96452.1"
                     /translation="MTLPERFPPITAKCPHMLHGGDYNPDQWMHTPEVWDEDMRLMRL
                     AGCNVMSVGIFSWAALEPREGHFEFGWLDTIMDKLAANGVYVVLATPSGSKPPWLSEA
                     YPEVCRVNAQGQREPHRERHNHCRTSPVYREKCRIINTQLATRYKDHPALVVWHVSNE
                     YNGGD"
BASE COUNT          383 a          521 c          613 g          348 t
ORIGIN      
        1 gtgttgctcg aacggtgcac gcacatcctt gtgggcaacg aggtagcgcc gctggacgat
       61 gcgcgccaag cggcaagcgt ggggaatatc gaagcacttt ccgcgcaggc gttcagaacc
      121 atcgccgtcg cctaccggaa tctggaaggc gagggagtga tcccggccga ggaaggtgcg
      181 ccggtggatg agtcgcttga ggccgatctt gtgtacgtgg gaatcgtcgg aattctggat
      241 ccccctcgcc ctgaagcggc aaaagcggtg gtggaatcac accgtgcagg cgtgcgcgtc
      301 atcatgatta cgggagacca tcccaagacg gcgacgcgca tcgcctccga tctcggcatt
      361 gtggggaagg acgcaaccgc tctgacgggg atggagatag acaaactgga cgaggcagcg
      421 ttgcgcgaag ccgtgcgcac gacctccgtg tatgcccgcg tggcgccaga gcacaaactc
      481 cagatcgtgg atgcgttgca ggccggcggg aacgtcgtgg cgatgacggg cgacggcgtg
      541 aacgatgctc cggccttgaa aagggcggaa atcggcgtag cgatgggggt taccggaact
      601 gacgtgacaa aagaagccgg caacatggtg cttgcggatg acaatttcgc gacaatcgtt
      661 gccgcggtgc acgaaggccg tgtgatcttc gacaacatcc gcaagttcct gcggtatctg
      721 ctcagttcca acatgggaga agtgctggcg gtctttttcg gggtggtgct ctccacggtg
      781 atagggttga cttccggcga cggaggaatc gtgttgccgc ttctggcaac acagcttctg
      841 tggatcaacc tcgttacgga tgcagcgccc gcgatggcgc tgggagtaga cccggaaacg
      901 gaggacgtga tggcccgccc accacgaaaa ctcacggaca gggtgatcga cggccggatg
      961 tggaccacgg tgttgatcgg cggctcggtg atggctgcca tgacacttct caccctggat
     1021 atccttcttc cgggcggttt gttcgagggg aaggggtcac tggatactgc gcgcaccgcc
     1081 gcattcacgg tgctggtgtt cacccagctc ttcaacgtgt tcaactcccg gtctgagcac
     1141 gcaagcgcat ttcatcgtct gtgcgcaaac cgatggctgc tcggcgcggt actgctcgga
     1201 gtagtcctgc aggttgccgt agtccacatt ccattcatga acacggcatt cggtaccaca
     1261 ccgttgacgc gcgaacaatg gattctgtgc gtggtgatgg cgtcggcggt tctgtgggtg
     1321 gaagaggcgc gaaagcttgt gtggcgaaca acaaacctga aggggaagaa atgacgttgc
     1381 cagaacgatt cccacccata accgccaaat gcccgcatat gctccatggc ggagactaca
     1441 accccgacca gtggatgcac acgcccgagg tgtgggatga ggacatgcgc ctcatgaggc
     1501 tcgcgggctg caatgtcatg tctgtcggca ttttctcatg ggcggcactc gaaccgaggg
     1561 aaggccattt cgaattcggc tggctcgaca cgatcatgga caagctcgcg gccaacggcg
     1621 tgtatgttgt gcttgcgacg cccagcgggt cgaagccgcc gtggttgagc gaggcctacc
     1681 ctgaggtatg ccgcgtgaac gcgcaggggc aacgcgagcc gcatcgcgag cgccacaacc
     1741 attgccggac gtcgcccgtg tatcgcgaga aatgccggat catcaacacg cagcttgcca
     1801 cgcgctacaa ggaccacccg gcgctggtcg tatggcacgt ctctaacgaa tacaacggtg
     1861 gggac
//
LOCUS       DAOTNG010000456         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_48526_length_1862_cov_4.863863, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000456 DAOTNG010000000
VERSION     DAOTNG010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48526_length_1862_cov_4.863863"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="P5179_14380"
     CDS             complement(<1..1190)
                     /locus_tag="P5179_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96453.1"
                     /translation="MNFRPRNPNRHLWDTWFYDHNGEYHLFYLSNRIADDGRWIPWDA
                     FSLATSRDLLHWEEKGEVFHKSENPADWDSELITTGDIIRWRGRWYMTYRATQDRVEK
                     NNIIVSDDLLNWQRISPSPVLVTSEPYEHDPAQTINNYVEMRDVTITELPDGTLEGVF
                     TARLNSGPHTGRGVLARAFSADMLHWRMGEPLFAPGRFNVIEVPSRFRHGGRHYLLFC
                     VNSHLESALENRNYPSMTWCTGYAVSDRCDGGFDYVPEQVLGIGDAYVGRLVNAGGET
                     LFFQHLCGRRPAFALPKSVSFGADGTIRLGYWRGVDSLIRGEGIRGFSTLQFFEDGGK
                     TVCGGNIAPETCTLENAAGFGDAYLPGTFSDFVLDVDIQFDRCVSAGVLLNADVPSRG
                     AVMAT"
BASE COUNT          311 a          612 c          600 g          339 t
ORIGIN      
        1 gtcgccatga ctgccccgcg cgacggcacg tccgcgttca gcagcacacc tgccgacacg
       61 caccggtcga actggatatc cacgtccagc acgaagtcgg agaaggtgcc cggcaggtag
      121 gcgtccccga agccagcggc attttcgagc gtgcaggtct ccggggcgat gttgccgccg
      181 cacaccgttt tgccgccatc ttcgaagaat tggagcgtcg agaagccccg aatcccttcg
      241 ccacggatga gcgaatcaac gcctcgccag tatccgagcc ggatggtgcc gtcggcgccg
      301 aacgacacgc tcttcggaag cgcgaaggct ggccgtctgc cgcacaggtg ctggaagaaa
      361 agcgtttccc cgcccgcgtt caccagtctg cccacgtacg catcgccgat accgagcacc
      421 tgctccggca cgtagtcaaa cccgccatca caccggtcgg acaccgcgta gcccgtgcac
      481 caggtcatgg acgggtagtt gcggttctcg agcgccgact cgaggtggct gttcacgcag
      541 aacagcaggt agtgccgccc gccgtggcgg aaccggctgg gcacctcgat gacgttgaac
      601 cggcccggcg cgaagagcgg ctcgcccatg cgccagtgga gcatgtccgc cgaaaacgcc
      661 ctcgccagca ccccgcgccc cgtatgcggc cctgagttca gccgcgcggt gaacacgccc
      721 tccagcgtgc cgtcgggaag ctcggtgatc gtcacatccc gcatctcgac gtagttgttg
      781 attgtttgcg cggggtcgtg ctcatacggc tcactcgtta ccagcaccgg cgacgggctg
      841 attcgctgcc agttgagcag gtcatccgag acgatgatgt tgttcttctc cacgcggtcc
      901 tgtgtggcgc ggtaggtcat ataccagcgc ccgcgccaac ggatgatatc gcccgtggtg
      961 atgagctcgc tatcccagtc cgcgggattt tccgacttgt ggaacacctc gcccttttcc
     1021 tcccagtgga gcaggtcgcg gctggtggcg agggagaagg cgtcccacgg aatccatctg
     1081 ccgtcgtcgg cgatccggtt ggagaggtag aagaggtggt actcgccgtt gtggtcgtag
     1141 aaccaggtgt cccacaggtg gcggttcggg ttgcgcggac ggaagttcat ggtggctcct
     1201 cgcggggtat gtgtgcaggg ttacgaatcg gtcaagatgg ccaaggcgca ggtcatctcg
     1261 atacgcggcg atgccgcact cgatgacccg cgcgttccat cgtcattccc tcttgctcaa
     1321 ccgagcccga cgtgaaagcg ggaatccaga acgcggtccg agtgcctgcc cgcggcttgc
     1381 caggccgccc ggcccgttga gccgggctca gttatgtcgg cccttcaggc cttccggcgg
     1441 ccgcaaaggc gggtgccagg cgccaacggc gcgggatagc atagcccggt tcgcagggcg
     1501 gagtatgccc gtgctacgcc tcagggagcc attatcggtc ctacgcgtcc tatacgaccg
     1561 atgcgccccg acagacaggc ggtggtttgt cctcagggtg ccgaaaacgc cacgtgtcgc
     1621 gggacagaga ggaatgtccg tccctccccg gcgtcactgc cctcgtcggt ggggcaggca
     1681 ttcctgcgcc tgccgggcgg cctgcctgcc gttatcaccg cccccgattc ttcatcacca
     1741 cttgatacgg cccgcccgca gttctgcgca aggtacggcg gctgacggtg tgggaattaa
     1801 tcctcgttgc ggggtgcgcc gtacggggtc atgttcgatt gccgaccccc ggggcagatc
     1861 tt
//
LOCUS       DAOTNG010000457         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_49047_length_1848_cov_6.266592, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000457 DAOTNG010000000
VERSION     DAOTNG010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49047_length_1848_cov_6.266592"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1376
                     /locus_tag="P5179_14385"
     CDS             99..1376
                     /locus_tag="P5179_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS96454.1"
                     /translation="MGNCYDVIVAGGGTAGTAAAIAASRRNHKVLLIEEQNCLGGTST
                     SGGVGEWFASLQGMGNIFDAVVAGLEKFGARFAKPRHFNPEVLKSAWQLLAEEAGVQI
                     LFHASVCGAERSGRAVSRIEVASCSRLFSAEAKYFVDATGEGDLSALAGAEYMQGEPG
                     TRKVLHMSLVAWMYDTGALQTPYLPASLEPVRSREELPGLGAGVLVDRNRVYLNATKV
                     LDRDPTDPWSLSDAEREARHQLLRVVHYLQTHEYPTFALASSGARIGIREGRRIVGDY
                     VLSRSDILNRREPLDFEDGVVVATSQIDFHSLKKAGNSGWRQPVLPYSIPLRCMLAKG
                     FPNLAVAGKCISVDQVVHSSCRMTPTCCAMGQAAGTAAALAIETGVADLRDVPISSLR
                     QTLNADGMELNPARHTAFAPHDTRLDADDHVGI"
     gene            complement(1361..>1848)
                     /locus_tag="P5179_14390"
     CDS             complement(1361..>1848)
                     /locus_tag="P5179_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRS96455.1"
                     /translation="PGGFDDLTRRLQRGSTVICLDPRVFADGDDQTAFLPLDRKGRFH
                     SIVGWLYLKDEWAKRHPIFDGMPAGGLLDYDFYREIIPDLLYSPGEESYEAVAGAIRA
                     SQGYSSGLMISVHRVGAGRLIINTFRIRENIGTHPAADMLLMNMLRVASRSDGDNHMP
                     T"
BASE COUNT          385 a          521 c          574 g          368 t
ORIGIN      
        1 agggtctgtc gagccttttt ggggcttgac ggaccctttc tgtgttgatc ggcgttcgtg
       61 ttggagtcca acgggtgcat tgtgacggta gggagaacat ggggaactgt tacgacgtca
      121 tcgtcgccgg gggagggacg gccgggaccg cagccgcgat agccgccagc cgtagaaacc
      181 acaaagtact gctgattgaa gaacagaact gcctcggcgg aacgtccact tcgggcggag
      241 tcggcgaatg gtttgcctca ctgcaaggga tgggcaacat attcgacgct gtcgtcgccg
      301 gtctggagaa gttcggcgcc aggttcgcga aaccccgcca tttcaatccc gaagtgctca
      361 aaagtgcctg gcagcttctg gcggaagagg ccggggtaca aatcctcttc catgcgagcg
      421 tatgcggtgc agagcgctct ggacgtgcgg tctcgcgcat tgaggttgcc agttgttctc
      481 gattgttttc ggcggaggcg aaatactttg tcgatgcaac gggcgaagga gatctgagcg
      541 cgctggcggg cgcggagtac atgcagggcg agccaggcac ccgaaaagtg cttcacatga
      601 gcctggtggc gtggatgtac gataccggcg ccctccagac gccctatctc cccgcatcgc
      661 tcgaaccggt tcgaagcagg gaggaattgc cgggattggg cgcgggagta ctggtggacc
      721 ggaacagggt gtatttgaac gccacgaaag tgcttgatcg tgaccccaca gacccctgga
      781 gcctgagcga cgcagaacgg gaagcgcggc accagttgct tcgtgtcgtt cactaccttc
      841 agacgcatga ataccccacg tttgcgctcg catccagcgg ggcgcgcatc ggtatccggg
      901 agggccgaag gatcgttggc gattacgttc tctcccggtc ggatatcctg aacagacggg
      961 aacctctgga tttcgaggac ggcgtggtgg ttgcaacaag tcagatcgac tttcacagcc
     1021 tgaagaaagc cggcaactcc gggtggcgcc agccggttct cccctatagc atccctttgc
     1081 ggtgcatgct tgcaaaggga tttccaaatc tcgctgtggc gggaaagtgt atcagtgtcg
     1141 accaggtagt acacagcagt tgcaggatga caccgacctg ttgcgcaatg ggacaggccg
     1201 ctggcacagc cgccgccctt gcgatagaaa caggagttgc agacctgcgg gatgtgccga
     1261 tttcgagtct tcgccagacg ctgaacgcgg atggcatgga gctgaacccg gcacgccaca
     1321 ctgctttcgc gccgcacgat acccgccttg atgccgatga tcacgtcggc atatgattgt
     1381 ccccgtccga gcgggatgca acgcgcaaca tattcatgag caacatgtcc gccgcgggat
     1441 gagtgccgat gttctccctg atgcggaagg tgttgatgat caaccttccc gcacccacgc
     1501 gatgaacgga tatcatcaac cctgaggaat acccctgcga tgcacgaatt gctcccgcca
     1561 ccgcctcgta tgattcctcg ccaggcgagt aaagaaggtc cggaatgatc tcccggtaga
     1621 aatcgtaatc cagaaggcca cccgccggca tcccgtcgaa gatcgggtgt cgttttgccc
     1681 attcatcttt gagatacagc cagccgacaa ttgaatggaa cctgcctttc ctgtccagcg
     1741 gaaggaacgc cgtctggtcg tcgccgtcag caaacacgcg cggatcgagg catatgactg
     1801 tcgagcctcg ctgcagacga cgggtgagat cgtcgaatcc tcccggtg
//
LOCUS       DAOTNG010000458         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_49088_length_1847_cov_4.126674, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000458 DAOTNG010000000
VERSION     DAOTNG010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49088_length_1847_cov_4.126674"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="P5179_14395"
     CDS             <1..1820
                     /locus_tag="P5179_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96456.1"
                     /translation="DGPLPMGPVTIYVSTSGDTMVTSDGSFGTIKDAIAQADFPVIQP
                     GDSVSSTPLDLSGATLRSPAHVFTRAQTQAGTATGNQMLAASFTVTVDISALEVESAR
                     AKIPRQTFSRSREVNFASDRIQIYSVKLARSVVADTNRFTLNIVNNLNTPLEVSYDLP
                     DFTIPRAPVTGAQHLQNVSVGSGGASDYARAMIALGARETKTVVFDLSDAVIENPTAP
                     KTPIDRVRVNLTTTVLGTGDTFVDIVSTDYVQVGADMSALAVQRVEGNVAPSQPPINV
                     TIQPVDFDVTQNLPAGLTGLAAEEITVELDVTAYETQVDADIGLVLTVANPAGGAPAA
                     QYTKTFSQQFRPGVNRHIRVTQDSLDAFGNSPVDIINAALNNLFASGQTKVEVSGNVN
                     IRGEIVLVRDSSRVDVKDVRVESPLKFTVPQLTFDAKAETEEGFEIDIDESVRNDLVP
                     RARSATLVADVENHFPMGATLVVFASPDPLFSTLRALYPASAKTPVAQIPDTLRIPQG
                     GPVTGEADAIARKAVFKLFTVSLPNPQRLSDGRVDHANPGRATVEVRLDDQLQLFSYE
                     KLYLMPRIQLLDAPGLVQLSPSDYVKVAIWMQIAGATKR"
BASE COUNT          398 a          551 c          514 g          384 t
ORIGIN      
        1 acgacgggcc tttgcccatg gggcctgtaa ccatctacgt gagcacgtct ggcgacacaa
       61 tggttacctc cgatgggtcc ttcggaacca tcaaagacgc gattgcgcag gccgattttc
      121 cagtcatcca accaggggat tccgtctcct cgactccgct cgacctttct ggagcaacgc
      181 ttcgcagtcc ggctcatgtc tttacacgag cccagacgca ggctggcacc gcgacgggca
      241 accagatgct tgccgcgtcg tttacggtta ccgtggacat ttctgcgctg gaggtggaat
      301 cggcccgcgc gaaaataccc cggcaaactt tttcacgctc gcgagaagtg aattttgcca
      361 gcgacaggat tcagatttac tcggtgaagc ttgcccgttc tgtcgttgcc gacaccaacc
      421 gcttcacttt gaacatcgtg aataacctca acactcccct ggaagtgtcc tacgatctgc
      481 ccgattttac catcccgcgg gcgccggtta ccggagcgca gcatcttcag aatgtgagcg
      541 tcggctccgg gggtgcctcg gattacgctc gagcaatgat cgcgctcggc gcacgcgaaa
      601 ccaaaactgt cgtgttcgat ctcagtgacg cggtcatcga gaacccgact gcgccaaaaa
      661 cgcctatcga ccgtgttcgc gtgaacctca ccaccacagt gctcggcacg ggggacacct
      721 ttgtagacat cgtttccacg gactacgtgc aggtaggtgc cgatatgagc gcgttggcgg
      781 tgcaacgtgt ggaagggaat gtcgcgccct cgcaacctcc aatcaacgtc acgatccagc
      841 cggtcgattt cgacgtaacc caaaatctgc ctgccgggtt gacaggtctg gcggccgagg
      901 agatcactgt agaactcgac gtaaccgcgt acgaaacgca ggtggatgcc gacatcgggt
      961 tggtgttgac cgtggcaaac cctgcgggcg gagcacccgc ggcgcagtat accaaaacgt
     1021 tttcgcagca gtttcgaccc ggagtgaacc gccacatccg tgtgacgcag gacagtcttg
     1081 acgcgtttgg caactccccg gttgatatca ttaatgccgc cctcaacaat ctgtttgcca
     1141 gcggccagac caaagtcgag gtctcaggaa atgtgaatat tcgcggcgaa atagtactgg
     1201 tccgagattc cagtcgcgta gacgttaagg acgtacgtgt cgaatccccc ctcaaattca
     1261 cggttcctca actcaccttc gatgcaaaag cggagaccga ggaagggttt gaaatcgaca
     1321 tagacgaatc tgtgcgaaac gatctggttc ctcgcgcccg gagcgcgacc ctcgtggcgg
     1381 acgtggagaa ccatttccct atgggggcca ccctcgtcgt gttcgcaagt cctgaccctc
     1441 tgttctcaac cctgagggcc ttgtacccgg caagcgcgaa aactccggtg gcacaaattc
     1501 ctgacacact caggattccg cagggtggac ctgttacggg ggaagccgac gccatagcca
     1561 gaaaggccgt gttcaagctc tttaccgtca gcctcccgaa cccgcaacgc ttgagtgacg
     1621 ggagggtaga ccacgccaat cccggccggg cgacggtgga ggttcggctc gacgaccagt
     1681 tgcagttgtt ctcctacgaa aaactttatc tgatgccgcg tatccagctt ctcgatgcac
     1741 cgggtctcgt tcagttgtct ccttctgatt atgtgaaggt tgcaatctgg atgcagatcg
     1801 ccggcgcgac caagaggtaa cgcccatgga ttgcaggaaa ggcgcgt
//
LOCUS       DAOTNG010000459         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_50018_length_1823_cov_4.342760, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000459 DAOTNG010000000
VERSION     DAOTNG010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50018_length_1823_cov_4.342760"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            650..943
                     /locus_tag="P5179_14400"
     CDS             650..943
                     /locus_tag="P5179_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS96457.1"
                     /translation="MRPTSDLLRKERAAILQIARKYGARNLRVFGSVARGEDTEMSDI
                     DILVDFAPDVSLFDHAALMEELESALGRKVDVVSEHGLRPRMRERVLKEAVPL"
     gene            940..1287
                     /locus_tag="P5179_14405"
     CDS             940..1287
                     /locus_tag="P5179_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS96458.1"
                     /translation="MRSDRERLLDALDAISRIREKVTEDLDSFLADEMVQVWVTHHLE
                     ILGESIARLSDELKTHYPRVPWSRAVAMRNILVHHYFSVDPRQVWNTVANDLPKLQRD
                     LEAILKSMPEEST"
     gene            complement(1487..>1823)
                     /locus_tag="P5179_14410"
     CDS             complement(1487..>1823)
                     /locus_tag="P5179_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96459.1"
                     /translation="VTRIRSAEERESTCNSARGAGRLEAGVAIGVLSTGGAQEMRANA
                     TAKGVIRDMNMQSPGCTVAVRWRGLLRQALAAGYAAPGPRTKAVIPAQAGIQVCGRTH
                     STSGMARAE"
BASE COUNT          346 a          559 c          590 g          328 t
ORIGIN      
        1 caacggggcg aaataccaca gcccggctcg cagggccggg cggcctcgtg gctgacacgt
       61 atcgccgggt gaggacccga cggagcgggt ccctccagag atatggtgcc cggcgaaatg
      121 ttcggtcgtc gagtggcgaa cgcggttcgc acgccgcgtt cgacgtatcg agacgacggg
      181 cgatggggag agcggctccg caaacgtgtc aggcgattct tcgctgcgtc cgccgcgcag
      241 gcggcgctca gaatgacgcg caagtcgcaa tagtcattcc cgcgcaggcg ggaatccaga
      301 cgacccgttc aggcctgcac accaacgccc gtggcgacgc ccgcgggctt agctatatcg
      361 gcccttcagg ccttgccagg tgcgtgaagg gacgctgtaa gccccaacgg ggcgaaatac
      421 cacagcccgg ctcgcagggc cgggcgacag tgccgccgaa acgcatcgcg gaaccaggac
      481 ccgacgtagc gggtccctcc cgcgaggtgt tcccgcgccg gtcgttgcgg cggaaacgcc
      541 ccctgacgtg gggattctgg agacctcgtc gaacgcgggc aagagcattc atttggctgc
      601 atttctgcgt gattcttcgt aatttcgtat cagaaacgga ggaccacaga tgcgaccaac
      661 ttccgatctt ctgaggaaag aacgcgccgc catcctgcag atcgcgagga agtacggggc
      721 acgcaatctt cgtgtgttcg gctccgtcgc gcgcggtgaa gacaccgaga tgagcgacat
      781 agacatcctt gttgacttcg cgccggacgt ttcgcttttc gatcatgcgg ccctgatgga
      841 ggaactcgaa tccgcgcttg gcaggaaagt ggacgtggta agcgagcacg ggctgcgacc
      901 gagaatgcgc gagagggttc tgaaggaggc ggtgccgttg tgaggagtga tcgggaacgc
      961 cttctggatg cgctggacgc gataagcagg atccgggaga aggtgaccga ggacctcgat
     1021 tcgttccttg cggacgaaat ggtacaggtg tgggtcaccc accacctcga gattctggga
     1081 gaatcgatcg cgcggctgtc tgacgaactg aaaacgcact acccgcgcgt accgtggtcg
     1141 cgtgcggtgg caatgcggaa tatcctcgtt caccattact tcagcgttga cccccgacag
     1201 gtgtggaaca cggttgccaa tgacctcccg aaactccagc gggatctgga ggccatcctg
     1261 aagtccatgc cggaagagag tacctgacgg ggcgtggcat tttgccgggt attcaggcat
     1321 tgaggacccg acggagcggg tccctccaga aggttggtgt cgcgaggcag gcgcatttat
     1381 cgtcgccggt gggtgccgtc attcccgcgc aggcgggaat ccagccggaa gccgtcggtc
     1441 cggaatttcc ggggtcgtgc gcaatggtgc acacaacgcg ccttgcctat tccgcccttg
     1501 ccatgccgct tgtggagtgt gtacggccgc aaacctggat tcccgcctgc gcgggaatga
     1561 cggcttttgt tcgcgggccg ggcgcagcgt atcctgcagc cagggcctga cgaagcaggc
     1621 ccctccaccg gacagccaca gtacaaccag gtgattgcat gttcatgtcg cgaatgaccc
     1681 ccttcgctgt cgcatttgcc ctcatctcct gcgctccccc agtcgaaagg acaccgatgg
     1741 ccacgcccgc ttccaatcgg cccgcgcctc tggccgagtt acacgtcgac tccctctcct
     1801 ccgctgaacg aatccgcgtg acg
//
LOCUS       DAOTNG010000460         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_50256_length_1818_cov_2.267158, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000460 DAOTNG010000000
VERSION     DAOTNG010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50256_length_1818_cov_2.267158"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="P5179_14415"
     CDS             complement(<1..1565)
                     /locus_tag="P5179_14415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS96460.1"
                     /translation="MKGEAFLRMCAAKPPFSRMHPKLMGFFKAYLSHEKVIPFRGRYV
                     LNTHFPPYPGPAFDTMVRNFEELGEAGTRRLFSVTLAVTNRCPYRCWHCYNAGRSQKD
                     VPLSIWKPVIARLQELGAANVTISGGEPLLREDLEEFVASFDKRTSLTLNTTGRGLTP
                     ERAGSLRDAGLFAVGVSVDSSVAQQHDRMRGYPGAFGIALDAIKVASDCGLYPYIISV
                     ATREFLEPDHFHAFMTFARETGALEVHLLEPSATGTLAGNRSVTLTDAERRRILQYQR
                     EVAEDEDLPILSTFTYLESPDAFGCGAGLTHLYIDGSGEVCPCNLVPLSFGTITREPL
                     DAILERMGQHFKTPRTGCVGRTLCRHIDSAELPLAPDASSEICRKHLPRRHGVPRFFR
                     VRNEARGEVDQEELRDAYDGIHQYYDEFWVKEAGKPVESLIAALSLSGNERVFEAGCG
                     TGFATVLLAAQLRDVGHLVAVDLSEGMLTEARARARAQGFGGIDFRRGDALQELLTSR
                     PFDLVFSSWVLGY"
BASE COUNT          349 a          585 c          553 g          331 t
ORIGIN      
        1 atgtagccca gaacccagct tgagaacacg agatcgaagg gccgcgacgt gagaagctct
       61 tgcagtgcat caccacggcg gaaatcaatt ccgccaaatc cttgcgcccg cgccctcgcc
      121 ctcgcctccg tcagcatccc ttcggaaaga tccacggcaa caaggtgacc gacgtcgcgc
      181 agttgtgccg caaggagcac cgtggcgaac ccggtgccgc acccggcttc gaacacccgc
      241 tcattcccgg atagcgacag cgccgcgatg agactttcca ccggtttgcc cgcctctttg
      301 acccagaact cgtcatagta ctggtggatc ccgtcgtacg catcgcggag ttcctcctga
      361 tccacctctc ccctcgcctc gtttcgtaca cgaaagaaac ggggcacgcc gtgccgccgc
      421 ggcaggtgtt ttcggcaaat ctcggatgaa gcatcgggcg cgaggggcaa ttccgcgctg
      481 tctatgtgcc ggcagagcgt gcgaccgacg caccctgtcc gcggcgtttt gaagtgctgc
      541 cccatgcgtt ccaggatggc atccagcggc tcccgggtga tggtgccgaa ggaaaggggt
      601 accagattgc aggggcacac ttcgccgctg ccgtcaatgt agaggtgggt tagtcccgcg
      661 ccgcagccga atgcatcggg cgactcgagg taagtgaacg tggagagaat cggaaggtcc
      721 tcgtcttcgg ccacctcgcg ctgatactga aggatgcgcc gcctctcggc gtcggtaagt
      781 gtcacggacc ggttcccggc aagcgtgcct gtggcgctcg gttccagaag atgcacttcg
      841 agggcgccgg tttccctggc gaaggtcatg aacgcgtgga aatggtcagg ctcgaggaac
      901 tcgcgggtgg ccacggagat gatgtagggg tagaggccgc agtcagacgc aaccttgata
      961 gcgtcgaggg caatcccgaa cgctcccgga tacccgcgca tccggtcgtg ctgttgggca
     1021 accgaggaat ccacactcac accgactgcg aaaagccccg catctcgcaa cgagcccgcg
     1081 cgttccggtg tcagccctct gccggtggtg ttcagcgtga gcgacgtcct cttgtcgaag
     1141 ctggccacaa actcttccag atcctcgcga agcagcggct cgcctcctga gatcgtcaca
     1201 ttggccgctc cgagctcctg aagtcgggca atcaccggct tccagatcga gagcgggaca
     1261 tccttctgac tcctgccggc gttgtagcag tgccagcacc ggtacgggca ccggttcgtc
     1321 acggcgaggg ttacggaaaa cagtctccgg gttcccgcct cgcccagttc ctcaaaattc
     1381 cgcaccatcg tatcaaacgc gggaccgggg tacgggggga aatgggtatt gagcacgtac
     1441 cgaccgcgaa acgggatcac cttttcgtgt gagaggtacg ccttgaagaa ccccatcaat
     1501 ttcgggtgca ttctggaaaa gggcggtttc gccgcgcaca tccgcagaaa ggcttcacct
     1561 ttcatcgttg gcaccccttt cgccttgctc ccaaagattt gccggcccgt gggacgtgac
     1621 acattgcgcg cgcggttccc cgtctgatgt ggaatatagc cgaatcggaa accaacggaa
     1681 ggagcgtggg cacgcgtgac cgggtgcgtc cgtcccaccg gctgcaaacc ggcctgccgg
     1741 tgcgcttcgg cacgttgcgg cagcccttcg acaggctccc gccagcgtga gcgggctcag
     1801 acaccgccac actacccg
//
LOCUS       DAOTNG010000461         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_50940_length_1800_cov_5.665903, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000461 DAOTNG010000000
VERSION     DAOTNG010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50940_length_1800_cov_5.665903"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="P5179_14420"
     CDS             complement(<1..1145)
                     /locus_tag="P5179_14420"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS96461.1"
                     /translation="MPKITFSGATRTVTGSMHLLETGGKRILLDCGLFQGKREEAFKR
                     NRHFPFDPASITDVVLSHAHIDHSGNLPTLVRKGFTGRIHATHATRDLCAVMLLDSAH
                     IQEKDVEFVNRKRARKGEEPFNPLYTMVDAHRTIDRFVSYGYHQPFAIGGTSIEFFDA
                     GHILGSASVKLTFKGRGTPSTLAFSGDVGRKNLPILRDPEPFDHADWLICESTYGARL
                     HHPPSEVENRVAEVVNTTYRQRGKIIVPAFSVGRTQEIVYALNRLANANRIPRLPIYV
                     DSPLSTNTTEVFRLHPECYDEETRQFLEEEGDPFGFSQLQYTRTVQESMALNSRTDPM
                     IIISASGMCEAGRILHHLRNNIEDNRNTIMIVGFQAENTLGRRLVDG"
     gene            complement(1164..>1800)
                     /locus_tag="P5179_14425"
     CDS             complement(1164..>1800)
                     /locus_tag="P5179_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRS96462.1"
                     /translation="ISPVFGAAVFAVLLALAAGSAHNRGATAGSDTAIGVGFALSMAL
                     AVLLISLIPESASRVWGLLMGDVLLITNQDLVLLAFLTLCLAIVFVVFWKQFLFVSFD
                     LDGARAFGINADRFNYLLFALIGLSSALLVKAIGSILVFAMLSAPAATALLISDSVKK
                     TLWWSFAIAFVAGILALVLSYFTALSVSAIAAFLVTTAYFAVRIWRFASAQ"
BASE COUNT          423 a          486 c          519 g          372 t
ORIGIN      
        1 tgaccatcca ccagccgtct tcccagtgtg ttctccgcct ggaatccaac gatcatgatg
       61 gtgtttctgt tgtcttcaat gttgttgcga aggtggtgca ggatgcgacc cgcttcgcac
      121 attccggacg cagatatgat gatcatcggg tcggtacgcg aattcagcgc catgctttcc
      181 tgaacggttc tggtgtactg aagctgcgaa aacccgaacg gatcaccttc ttcttccaga
      241 aactggcgtg tttcttcgtc gtagcattcg gggtgcaggc gaaagacttc cgtggtattc
      301 gtcgaaagcg gactgtccac gtaaatcggg agacgaggga ttcgattggc gttggcaagc
      361 cggttcaggg cgtagacgat ctcctgcgtt cttcccacgc tgaaggcggg aacgataatc
      421 tttcccctct ggcgatacgt ggtattcaca acctccgcca cccggttttc gacctcagaa
      481 ggcggatgat gcaatcgcgc accgtacgtg ctttcgcaaa ttagccagtc cgcgtggtca
      541 aacggctctg gatcgcggag aatggggaga ttcttccgtc ctacgtcgcc cgagaatgcg
      601 agagttgaag gcgtgcctcg ccctttgaac gtgagtttga cggaggccga cccaaggata
      661 tgccctgcgt cgaaaaattc gatacttgtg cccccgatgg cgaacggctg gtggtatccg
      721 tagctgacga accggtcaat tgtgcggtgg gcgtcaacca tcgtgtaaag agggttgaaa
      781 ggttcttctc ccttccgcgc gcgcttgcgg ttgacgaatt cgacgtcctt ctcctggatg
      841 tgcgcgctgt cgagcaacat taccgcgcaa agatccctcg tcgcatgagt cgcatgtatc
      901 cttccggtga agcctttgcg aacaagcgtc ggaagattcc cggaatggtc aatatgcgca
      961 tgggaaagaa cgacgtcggt gattgaggcc ggatcgaacg ggaaatgtcg atttcgcttg
     1021 aatgcctctt cgcgtttgcc ctggaacaac ccgcaatcga gcaggatccg cttgccgccg
     1081 gtttccagaa ggtgcatgga ccccgtcact gttcgagtgg ctccggagaa cgtgattttg
     1141 ggcataaaac ctccttgttc gttctattgc gcggacgcga atcgccaaat tcgtaccgca
     1201 aagtaggccg ttgtaacgag aaatgcggcg atggcgctca ccgaaagagc ggtgaaatac
     1261 gacaacacca gagccagaat acctgccaca aaggcgatcg caaaggacca ccagagcgtc
     1321 ttcttgaccg aatcagaaat aagtaacgcc gttgcggcgg gagcactcag cattgcgaaa
     1381 acgagtatcg atccgattgc tttcaccagc agcgccgagg aaaggccgat caacgcaaaa
     1441 agcaggtagt tgaatcggtc cgcgttgatg ccaaatgccc gggcgccgtc gagatcgaaa
     1501 gagacgaaaa ggaattgctt ccagaaaacg acgaagacga ttgcaaggca gagagtcagg
     1561 aaggcaagca gcacgagatc ctgatttgtg atgagcagga catcgcccat gagcaagccc
     1621 cacacacggc tggccgactc cggaatcagt gagatcaaca gaaccgccag tgccatcgag
     1681 agagcgaacc cgaccccgat tgccgtatcg gaaccggctg tagcgcctcg gttgtgcgcg
     1741 cttcctgccg caagtgcgag cagaacagca aagaccgccg cgccgaagac gggactgata
//
LOCUS       DAOTNG010000462         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_51175_length_1794_cov_3.822887, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000462 DAOTNG010000000
VERSION     DAOTNG010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51175_length_1794_cov_3.822887"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..860)
                     /locus_tag="P5179_14430"
     CDS             complement(144..860)
                     /locus_tag="P5179_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96463.1"
                     /translation="MAVNVRAGPDSNWFVWWEDENAPSSLAAERLFAALLEHRSIATA
                     ADSEGLNQAKLWEFTVTRDFHNGLGGVIAALEAAAEFGIEAVWNRAMELKQTAEAVTD
                     AARYDGVFREKALLGGKEILAALTEEGRTLERAFVCRSENLPDRTKVASWFQRVGTAV
                     RRMEQAGMDVDGVMLVIEGEQRYFLSSPRAVVPSVLCTIDQIRRLLEDADDHWREMRN
                     ANDEADEAEGNRFNLWSCWL"
     gene            complement(934..>1794)
                     /locus_tag="P5179_14435"
     CDS             complement(934..>1794)
                     /locus_tag="P5179_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS96464.1"
                     /translation="SVMEFKSAMDVQIAEKMLRFPLLGEKIEGSWNLVLSREFDMTND
                     SHLFRTEPGPGRLPLYEGKMIHQFDHRWGEPKYWIDEAEGRKALLGRERDTGQTLDYQ
                     CYRLAFRTVARSTDERTMIATVVPHSVYMGNSMSFVSFANDQTIIWSSAMLNAFTVDW
                     LLRGKVSANCNMFYVYDLPIPRFTPSDPWFWPVVSRAARLICTTPEFDDLAREVGLAG
                     HEDGATTPAERARLRAELDGIVAHIYGLTDEEFAHILRAFPLVPEATKTAALEAYRDV
                     AAGTITPERY"
BASE COUNT          348 a          580 c          464 g          402 t
ORIGIN      
        1 cgcagacctt cgcaactatc tttccagtag cattttttgc catatttgga tcatcctctt
       61 tgtcacgaaa aatagctatt attcgtgaca aaggcaaatg cacttatgtg gtatctcgat
      121 gcttccggca acgtacgggc atctcaaagc caacaggacc agagattgaa ccggtttccc
      181 tctgcttcat ctgcttcgtc gttagcgttc cgcatttccc tccagtgatc gtccgcatct
      241 tcgagtaacc gccgaatctg gtcaatcgtg cagagaacag acgggacaac cgcccgcggg
      301 gaggacagaa agtagcgctg ttcgccttca atcaccagca ttaccccgtc aacgtccatg
      361 cctgcttgtt ccatccgtcg gaccgcggta cccacccttt ggaaccacga cgcgaccttc
      421 gtcctgtccg ggaggttttc cgacctgcac acgaaggctc tttccagcgt ccgtccctcc
      481 tcagtcagcg cagcgagaat ctctttcccc cccaacaagg ccttctcgcg gaacactccg
      541 tcgtatctcg ccgcatccgt gaccgcttcc gccgtttgct tcaattccat cgcccggttc
      601 cacaccgcct cgatgccgaa ttccgccgcg gcctccaatg cggcgatgac tccgccgagt
      661 ccgttgtgga aatcgcgcgt tacggtaaac tcccagagtt tcgcctggtt gaggccttca
      721 ctatcggctg cggttgcgat ggaacggtgt tccaagagag cggcaaatag gcgttcggca
      781 gccagggaag agggcgcgtt ctcatcctcc caccacacga accagttgct gtcaggtccc
      841 gccctcacat tgactgccat cgcgtgctcc tcatgcacaa cccgtagatt cccgccctta
      901 ctccccctct tccgcgtccg cgcccatgcc cgatcaatac ctctcgggcg tgatcgtccc
      961 cgccgccaca tctcggtatg cttccagcgc ggccgttttc gttgcttccg gcacgagggg
     1021 aaacgcgcgg aggatgtgcg cgaactcctc gtcggtgagg ccgtagatat gggcaacaat
     1081 gccgtccagc tccgcccgca ggcgagcgcg ttcggcaggg gtggtagcgc cgtcttcgtg
     1141 acccgccaga cccacctccc gcgccagatc gtcaaactcg ggcgttgtgc agatcaaccg
     1201 ggcggcacgg gaaacaacag gccagaacca cgggtcggac ggtgtgaatc gagggatcgg
     1261 gaggtcatac acgtaaaaca tgttgcagtt tgcagatacc ttgcctcgaa gaagccaatc
     1321 gacggtgaag gcgttgagca tcgcagacga ccagattatg gtttgatcat tggcgaatga
     1381 gacgaaactc atcgaattgc ccatgtacac cgaatgaggg acgaccgtcg cgatcatcgt
     1441 cctctcgtcc gtgcttctgg caaccgttcg gaatgccagc cggtagcact ggtaatccag
     1501 cgtctgcccc gtgtcccgtt ctcttccaag cagcgctttg cgaccttccg cttcgtcaat
     1561 ccaatatttc ggctctcccc agcggtggtc gaactggtgg atcatctttc cttcgtagag
     1621 tggcagccgc cccggccccg gttcggtgcg gaacaggtgg ctgtcgttgg tcatatcgaa
     1681 ttcgcgactt agcacgagat tccaactgcc ctcgatcttt tcccctaaca gcgggaaccg
     1741 gagcattttt tcggcgatct gcacgtccat cgcgcttttg aattccatga cgga
//
LOCUS       DAOTNG010000463         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_51482_length_1787_cov_3.489607, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000463 DAOTNG010000000
VERSION     DAOTNG010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51482_length_1787_cov_3.489607"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="P5179_14440"
     CDS             <1..813
                     /locus_tag="P5179_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HRS96465.1"
                     /translation="AYRYYLDEVMEPARLGRSDKSVFDELLTERQCARDADVVLSCVS
                     RALARVSRLITVAFLPTFDNGVLRRIELVSLGESRILVALEIKGGPVHTLTLEMNEPV
                     AQGLIGETARILNERLSGLTIGQIRTSIAERLSGISGGEQHVINVFLREGSDIFDIDS
                     RTNVHLEGRPNILAQPEFSDHTRLMQFMRLLDEKTLIQELRRRGVTRRTQVSIGTENS
                     LPELADCSLLTRGYQAGSLSGTLAIVGPMRMPYRRLVAVLEHAGGVAETLMS"
     gene            870..1685
                     /locus_tag="P5179_14445"
     CDS             870..1685
                     /locus_tag="P5179_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96466.1"
                     /translation="MRWRFLPGILLAAPLLWPGCCGDCIVGFNRRCPEVIADVPRHTP
                     NAFFAWAYGGHPGFEGTTTRPVTILFQKKAAADSGYFQLSCSPGADANYCGVVNRFCV
                     DAVSFDTAYGLARYRTPDSRGSAANDPAYYIALPNDSVTVTSNRIQSAISSSPTVYDT
                     TLVTLYANTTADPPTLMPDRTPLITLAWAKYPLATQAVLLTNDAQGNLLSGVVILAGT
                     TTNPTTWTYGAPAARVLRTSQPLQSGTPYTLTVWLLNNGNWSLAVDQVTFTPQ"
BASE COUNT          335 a          608 c          509 g          335 t
ORIGIN      
        1 gcataccggt actacctcga cgaagtcatg gaaccggcgc gcctcggccg ttccgacaaa
       61 tcggtgttcg acgagctcct gacagaacgc cagtgcgcgc gcgacgctga cgtggtcctg
      121 tcatgcgtct caagggcgct tgcccgcgta tcccgtctta tcacggtggc gtttctcccc
      181 acgtttgata atggcgttct gcggcgtatc gagcttgttt cgctgggcga aagccgcatc
      241 cttgtggcgc tggaaatcaa aggcggcccc gttcacacgc tgactctgga aatgaacgag
      301 cccgttgcgc agggactgat cggtgaaacc gcccgcatcc tgaacgaacg gctcagcggc
      361 ctgactatcg gccagatacg cacgtccatc gcggaacggt tgagcggcat ctctggcggc
      421 gaacagcacg taatcaacgt atttctccgc gaaggatcgg acattttcga catcgattcg
      481 cggacgaacg tgcatctgga agggcgcccc aacatccttg cgcaaccgga gttcagcgac
      541 cacacccgcc tgatgcagtt catgcgcctt ctcgacgaga aaacgctgat tcaggaactg
      601 cggcggcgtg gcgttacgcg gcgaactcag gtgtccatcg gaaccgagaa ttcgctgccg
      661 gaacttgccg attgcagcct gctgacgcga gggtatcagg cgggttctct ttcgggaacg
      721 ctcgccatcg tcggtcccat gcgcatgccg taccgccgcc ttgtcgccgt tctggagcac
      781 gccggaggcg tcgccgaaac gcttatgagt tgagtgcggt gggaaggaac cccgtcctga
      841 aaggatgcct cttcctgaaa ggaagattca tgaggtggcg cttcctcccc ggcattctgc
      901 tggcggcccc cctcctctgg cccggctgct gcggagattg catcgtcggg ttcaaccgcc
      961 ggtgcccgga agtgatcgcc gatgttcccc gccacacacc gaatgccttt ttcgcctggg
     1021 cgtacggcgg ccaccccggc ttcgagggca cgaccacccg ccccgtcacc atcctgttcc
     1081 agaaaaaggc cgccgcggat tccggctatt tccagctttc ctgctccccc ggtgctgatg
     1141 ccaattactg cggcgtggtg aaccgtttct gcgtggacgc cgtctcgttc gacaccgcgt
     1201 acggccttgc acggtaccgc accccggatt cccggggaag cgccgcgaat gatccggcgt
     1261 actacatcgc cctccccaac gactcggtta ccgtcacatc caatcgcatc cagtcggcca
     1321 tatccagttc tccaacggtg tacgacacga cgctcgtaac gctctacgca aacaccaccg
     1381 ccgacccgcc caccctgatg ccggacagga ccccgctgat aaccctggcg tgggcgaaat
     1441 acccgcttgc gacgcaggca gttctcctca caaacgacgc gcagggtaat ctcctatccg
     1501 gcgtggtgat actggcgggg accaccacca accccaccac gtggacgtac ggcgcccccg
     1561 ccgcgcgcgt gctgcgcacc agccagccgc tgcaatccgg cacaccgtac acgctcaccg
     1621 tctggctgct gaacaacggg aactggagcc tcgccgtgga tcaggtgacc ttcacgcctc
     1681 aataacccgc tccgagggcc cgcatgacaa aacgtgaggt ggttcgaaca gccattcaag
     1741 gccggcaacc accctatgtg ccttggtcgt tcgggttcac cgtggaa
//
LOCUS       DAOTNG010000464         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_52370_length_1765_cov_3.632749, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000464 DAOTNG010000000
VERSION     DAOTNG010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52370_length_1765_cov_3.632749"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="P5179_14450"
     CDS             complement(<1..1744)
                     /locus_tag="P5179_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HRS96467.1"
                     /translation="MLNNIIGFDLNPLAVMAARTNYLMAIRGLVRHADNVELPVYLCD
                     SVMTPKDFSTAAAAFDYVPVTTAVGQFNIPNEVLKEREKFHTYARTLHQCIENDYTFG
                     EFAQRCAAEGVPTTQHEHHRKVYDSLLEMKRQDRNGIWARIIKNAFAPLFVGTVDFVA
                     GNPPWVNWENLPEEYRNKMKPLWQEYGLFTLSGSAGRLGGGKKDLSMLFVYSGVDRFL
                     RNGGKLGFVITQSVFKTQGAGDGFRRLRFARDSRTTYLKPLAVADLSAIQVFEGATNR
                     TAVFVCEKQRESFQYPVSYGIWRGPSRISQDVSLAEARSLVNVLQVAALPVDPEKPNS
                     PWLTAPRHALRGLLKVIGKSDYQAYAGVFTGGLNGCYWIRILDRLSNGDLLIENLHDV
                     GKLKVEHVQTVIEPDLVYPLLRGRDVKRWHAEPSAHIILAQDPVTRTGIRETVMKVKY
                     PKTYAYLKRFEGDPKKPERGTLRGRATSFVRRPIENGAPFYTMFAVGEYTLAEWKVVW
                     NRIDTRLQAVAVGGDTEDPPVVPQETHTFVPISAQREAYYFSGALNSSPCDFLVRCYS
                     TSKGFASAHVLNVLR"
BASE COUNT          337 a          520 c          533 g          375 t
ORIGIN      
        1 tccgcagcac gttcagaacg tgagcggacg cgaagccttt gctggtcgag taacacctga
       61 caaggaagtc acacggagac gaattcaacg ctccggagaa gtaataggct tctctttgag
      121 cagaaatcgg aacgaatgtg tgcgtttcct gcggaaccac aggtggatcc tctgtatcac
      181 cgccgactgc aaccgcttgc agccttgtgt caatccggtt ccacaccacc ttccactccg
      241 ccagcgtgta ctcccccacg gcgaacatgg tgtagaaggg agcaccgttc tctatggggc
      301 ggcggacgaa actggtcgcg cgccccctca acgttccacg ttccggcttc ttcggatcac
      361 cctcgaaccg tttcaggtac gcgtaggttt tggggtactt caccttcatg acggtttccc
      421 ggatacctgt acgggtcacg gggtcctgcg cgaggatgat gtgcgcggag ggttccgcat
      481 gccagcgttt cacgtcccgg ccgcgaagga gtgggtacac gaggtcgggt tcgatcaccg
      541 tctgcacgtg ttcgactttc agtttgccca cgtcgtgcag gttctcgatc aggaggtcgc
      601 cgttcgagag ccggtcaagg atgcgaatcc agtaacagcc atttaacccg ccggtgaaaa
      661 ccccggcata ggcttggtaa tcgctttttc cgatgacctt cagaagccct ctgagggcgt
      721 ggcgcggcgc ggtgagccag ggggagttgg gcttttccgg gtcaaccggc agtgcggcca
      781 cctgcaagac attgacgagg gagcgcgctt cggcaagcga tacgtcctga gagatgcgcg
      841 acggtccccg ccagataccg tagctgacgg ggtactggaa tgattctcgt tgcttttcgc
      901 agacgaagac cgcggtgcgg ttggtcgcgc cttcgaacac ctgtatcgcg ctcagatccg
      961 ccacggcaag aggtttgagg tacgtcgtcc gggaatcccg ggcgaatcgg agacgacgga
     1021 acccgtcgcc ggcaccctgt gttttgaaca cgctctgggt gatgacgaac cccagcttcc
     1081 cgccgtttcg aaggaaccgg tcgaccccgg agtagacgaa cagcatcgaa aggtctttct
     1141 tgcctccgcc gaggcgtcct gcggaacccg aaagcgtgaa caggccatac tcctgccaga
     1201 gcggcttcat cttgttgcgg tactcttccg gcaggttttc ccagttgacc cacggcgggt
     1261 tgcccgccac gaagtccacg gttccgacga agagcggcgc aaaggcgttc ttgatgatgc
     1321 gcgcccagat gccgtttcga tcctgccgtt tcatctccag cagcgaatca tacaccttcc
     1381 tgtggtgctc gtgttgggtg gtgggaacac cctcagcggc gcagcgttgc gcgaactcgc
     1441 cgaaggtgta gtcgttttca atgcactggt gcagggttcg cgcgtaggtg tggaacttct
     1501 cccgttcttt caacacttcg ttgggaatgt tgaactgccc gacggcggta gtcaccggca
     1561 cataatcgaa tgccgccgcc gcggtactaa aatccttcgg cgtcatgacg gaatcgcaca
     1621 gatacacggg cagttcgacg ttgtcggcat gtcgcaccag gccgcggatt gccatgaggt
     1681 agttggtgcg cgccgccatg acggccagcg gattcaggtc gaacccgatg atgttgttca
     1741 gcactctgcg aagcagttcc ttctc
//
LOCUS       DAOTNG010000465         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_52786_length_1755_cov_3.442353, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000465 DAOTNG010000000
VERSION     DAOTNG010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52786_length_1755_cov_3.442353"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1755)
                     /locus_tag="P5179_14455"
     CDS             complement(<1..>1755)
                     /locus_tag="P5179_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96468.1"
                     /translation="SSYFGADGQLSPIGRIQAAMGRFIEEHGSPGTMHAPVALMLDHY
                     AGWAMPRHLYSGSGYYGDTSYQVWGGIPYGAGDYLTHEVLSLVYPGYVDAGYYRDERG
                     FLSPTPWGDMMDCLLSDAAPWVLRQYGLIVIAGALSDENAGTETRDKLDAFMASGGHV
                     VATAENARRLWPELGIGDAIRVPAGTTVRLERTGEVFEEPLAFDLHTISAIPDEVVAE
                     AVLEPGSQPAAVRFTRGSGLLTVVLSPFGLNAEPTVSGPVPNEAEREFPCPFVMLQHV
                     RRVIADALATQQLFSVGEGLGFVTCRKGEGEYTVGVFNNSLEARPFQIRSLAGMIEDI
                     REIPLDQSEKGQPGYWPNGIQHDGGVSDEATIAGNDVRIFQVKLAKEAVRALPRVAPP
                     ERPRNRFLRLPRETGNVKEAILRMPTFFQHFDGVLIDWIALRARDPNVLREEAEWIGR
                     QKLRIAVDFSSGCNLFPDLQLLDTFLPAWERTLAVFDDVFGKMEVLGARDALLELHIG
                     PENEVSHERAHERFLVCVRDLCKRASTRSITLHLRPHPHKFCSTAAAMRQFVKEVGAT
                     NLRVAVDFGHGAQSEKPEA"
BASE COUNT          334 a          578 c          518 g          325 t
ORIGIN      
        1 gcctcaggct tctcactttg cgccccatgc ccgaaatcaa cggcaacgcg caggttcgtt
       61 gcccccactt ccttcacaaa ctgccgcata gccgcagccg tggaacagaa cttgtgcggg
      121 tggggccgca ggtgcagcgt gattgaccga gttgatgcgc gtttgcagag gtcacgcacg
      181 cagacgagga accgctcgtg tgcccgttca tggctcacct cgttctccgg tccgatgtgc
      241 agttcgagga gagcgtcacg cgcgccgaga acctccattt ttccgaacac atcgtcaaat
      301 accgcgagcg tgcgctccca cgcaggcaaa aacgtatcaa gcaactgaag atcggggaac
      361 aggttgcacc cggagctgaa atccaccgcg atgcggagct tctgccggcc gatccattcc
      421 gcctcttctc tcagaacgtt cgggtctcga gcccgcaacg cgatccagtc gatgagcaca
      481 ccgtcgaaat gctggaagaa ggttggcatc ctgaggatcg cttccttgac gttgcccgtc
      541 tcgcgtggta accgcaggaa cctgtttcgc ggccgctcgg gcggtgcgac gcgtggaagc
      601 gcgcgaacag cttccttcgc cagcttcacc tggaagatgc gcacgtcgtt gccggcgatc
      661 gttgcctcgt ctgatacgcc tccgtcgtgc tggatcccgt tgggccagta ccccggctgt
      721 cctttctccg attgatcaag cggaatctca cggatatcct ctatcatgcc ggctagtgaa
      781 cgtatctgga acggtcgcgc ctcgagggag ttgttgaaca cgccgaccgt gtactcgccc
      841 tctccttttc ggcacgtaac aaatcccagc ccctcgccca ccgaaaacaa ctgctgcgtc
      901 gccaacgcat cagcgatcac tcgccgcacg tgctgcagca tcacaaacgg gcaggggaac
      961 tcgcgctccg cctcattagg gaccgggccg gacaccgttg gttctgcgtt gaggccgaag
     1021 ggggacagaa cgacggtcag caacccgctc ccccgggtga accggactgc ggcaggctgc
     1081 gaaccaggct ccagcacggc ctcagcgacc acctcgtcag ggatcgcgga gatggtgtgc
     1141 aggtcaaacg ccagtggttc ctcgaatact tcgcctgttc gttcgagccg cacggtggtt
     1201 ccggcgggaa cgcggattgc gtccccgatc ccgagctccg gccacaggcg gcgcgcattc
     1261 tccgccgttg ccaccacgtg cccgcccgac gccataaatg cgtccagctt gtcgcgggtt
     1321 tcagttcccg cgttttcatc ggacaaagca cctgcaatga cgatgagccc gtattgccgc
     1381 agaacccacg gtgctgcatc cgagaggagg caatccatca tgtcgcccca cggggttggc
     1441 gacaggaagc cccgttcgtc ccggtagtac ccggcgtcaa cgtagccagg atacacgagc
     1501 gacagcactt cgtgggtaag gtagtcgccc gcgccgtacg ggattccgcc ccagacctgg
     1561 taggacgtgt cgccgtaata cccgctgccg gagtaaaggt gtcgcggcat cgcccagcct
     1621 gcgtaatgat cgagcatgag agccacggga gcgtgcatgg tgccgggcga gccgtgttct
     1681 tcgatgaacc tgcccatggc agcctgtatc ctgccaatcg gcgaaagctg cccgtccgcg
     1741 ccgaagtacg acgac
//
LOCUS       DAOTNG010000466         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_52822_length_1754_cov_4.732784, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000466 DAOTNG010000000
VERSION     DAOTNG010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52822_length_1754_cov_4.732784"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1409
                     /gene="cfa"
                     /locus_tag="P5179_14460"
     CDS             279..1409
                     /gene="cfa"
                     /locus_tag="P5179_14460"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HRS96469.1"
                     /translation="MVSDTVRELLDHAGIHINGEEAWDIRVHDNRFFGRVLAEKNLGL
                     GESYMDGWWDCPRIDEMICRLLRAGVEDRVRGNLRYALPFIRAYLFNLQKVSRARIIA
                     ERHYDTGNDLFFSFLDPYNQYSCAYFEGTDDLNRAQRQKMALIAGKLGLSKTDTLLDI
                     GCGWGGLARYSAETTGCRVTAVNISREQISFAREFCKGLPVEVVECDYRSIHGKFDKI
                     VSVGMFEHVGRKNYRTFMEVAWRSLKDDGVFLLHTIGSLRSKSGTDPWIEKYIFPNSM
                     LPSIAQISKAAEGLFVIEDLHNLGPHYDKTLMAWHSNFQRNWPQLAEKYGERFKRMWE
                     YYLLSCAGAFRARGIQIWQIVMTKHGTGTAQPQCRFVPALPE"
     gene            1543..>1754
                     /locus_tag="P5179_14465"
     CDS             1543..>1754
                     /locus_tag="P5179_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96470.1"
                     /translation="MAELDSRLRGNDGKSRATAENRAERETGGDKGWIPAFAGMTAGR
                     RQLFKTSATLALLNRRQSAACVTFFSL"
BASE COUNT          388 a          449 c          574 g          343 t
ORIGIN      
        1 gaaccgggtt cggtacgtga tttgggacga cgggaaagcg atcccggccc ttctagctac
       61 cgaatatgat tcggtatgca gcaggaatgg attcatcctg ctgaaacgca agttcgatct
      121 tccggacgat gcgaggcggg ggtagaatgg caacttcgtg cagccttcga attcgttacg
      181 tcgaacaatc ggcgaatatc atggaacagg aggggtagca ggaaaccggg caaagtgcct
      241 taccggtcgg cctgagccaa tcagggagga ctggagcgat ggtatccgat actgtgcggg
      301 agcttctgga tcacgcgggt atccatatca acggtgaaga ggcatgggac atcagggttc
      361 acgacaaccg gtttttcggt cgagtgctgg ccgaaaagaa cctcgggctg ggcgaatcct
      421 acatggacgg atggtgggat tgcccccgga tcgatgagat gatctgccgg ctcctccggg
      481 ccggtgttga ggacagggta cggggcaacc tcaggtacgc cttgcccttc atccgggcgt
      541 atctgttcaa tctgcagaag gtctcccgcg ctcgaattat cgccgaacgc cactacgaca
      601 ccggtaacga cctgtttttc tccttcctcg acccgtacaa ccagtacagc tgcgcgtatt
      661 tcgagggaac cgacgacctg aatcgggcgc aacggcagaa gatggcgctg attgcgggaa
      721 agctggggct ctcaaagacc gacacgctgc ttgatatcgg gtgcggatgg ggtggcctag
      781 cgcgatactc cgcggagacg acgggatgca gggtgactgc ggtgaacatt tcgcgggagc
      841 agatcagttt tgcccgcgag ttctgcaagg gcctgccggt ggaggttgtg gagtgcgatt
      901 accggagcat tcatgggaaa ttcgacaaaa tcgtttccgt ggggatgttc gagcatgtgg
      961 gccggaagaa ttaccggacg ttcatggaag tggcgtggcg ctctctgaaa gacgacggtg
     1021 tattccttct gcacaccatc ggcagcctca gatcgaaatc gggtactgac ccctggatcg
     1081 agaagtacat cttcccgaac agcatgctgc cgagcattgc gcaaatatca aaggcggcgg
     1141 aggggctttt tgttatcgag gatcttcaca acctcgggcc gcattacgac aaaacgctca
     1201 tggcctggca cagcaacttc cagagaaact ggccgcaact ggcggagaaa tacggcgagc
     1261 ggttcaaacg gatgtgggag tattacctgt tgtcgtgcgc cggcgcgttt cgtgcgagag
     1321 gaatacagat ctggcagatc gtgatgacca agcacggaac cggaacggca cagccgcagt
     1381 gccggttcgt gccggcgttg ccggaataag gcaggagtaa gggttctcac cggagattta
     1441 cgcggcgcgc acgaatgggc gtggtcagag aggcgacaga gaggtgtgcg tcaggggcct
     1501 cggggggtac gcccccgatc agagttcctg ccttccgggg taatggcaga actggattcc
     1561 cgccttcgcg ggaatgacgg caaatcgcgg gcaaccgcag aaaatagggc ggagcgggaa
     1621 accggcggcg acaaaggctg gattcccgcc ttcgcgggaa tgacggcggg ccgcaggcaa
     1681 ttgttcaaaa cgagcgccac gttggctttg ctcaatagac ggcagtccgc cgcgtgcgtt
     1741 acgtttttct ccct
//
LOCUS       DAOTNG010000467         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_53030_length_1749_cov_4.203070, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000467 DAOTNG010000000
VERSION     DAOTNG010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53030_length_1749_cov_4.203070"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..992
                     /locus_tag="P5179_14470"
     CDS             132..992
                     /locus_tag="P5179_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96471.1"
                     /translation="MTRFEKRCSNAVCGGTRIPSIACAFMATLVGILVLSSCGTRRAR
                     VHGTMYVPPGLQGNAQWGEVWLVHDSPELEYDLKERERLVTLALLGKDVEFLRRQLPA
                     LIQLGVLREKINILNRGQIPTAITLPPRREITALTPFDSIFQGIVVESRPATAPELAA
                     GLDKLKDELRDSVEAIHRRLQTSMDSLRAARAEQKRIMFAGANGSATPKRVMAAPVGL
                     DGRFSFSDVPPGKYRLHAQYRLQQWFVSVPLEVGRSDVQKDIPRLPGVLLDSRAIINT
                     EALCAKILAL"
     gene            1016..1435
                     /locus_tag="P5179_14475"
     CDS             1016..1435
                     /locus_tag="P5179_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96472.1"
                     /translation="MAPLRWVIGWFALTPVEILVLAAVSATVFWQALPRYKEWTFRQL
                     VAEETGDVTQLNYALSGDTGLFVRCPARLDDCPDGSTTETCSLFSALSPEFRVASAWR
                     KENGDYLGPAGGRYRYLPGECRLAPVGKESQGGKRRH"
     gene            complement(1451..>1749)
                     /locus_tag="P5179_14480"
     CDS             complement(1451..>1749)
                     /locus_tag="P5179_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRS96473.1"
                     /translation="KLQDQLSRAIVLDDQSLPEGKAHIGRRVDLEDLNTGKRFSYTLV
                     SEAESDFASGKISTGSPVGRGLVGHGAGEEVTVQVPAGTKRFRILRVVSSADTQ"
BASE COUNT          347 a          527 c          518 g          357 t
ORIGIN      
        1 gcgcgtatca gcggcggatc tgcgaaaggt acgcgcagga attgagagca caaaagaacc
       61 ccgactgaaa aaaatgtgcg gctcgcgggc cgcctcaatg tgatagatac gtgctcgtca
      121 ggagattcgc aatgacgcga ttcgaaaagc gatgctcgaa tgcggtgtgc ggtggaaccc
      181 gcataccgtc cattgcgtgt gccttcatgg cgacgcttgt cggcatactg gtgttgtctt
      241 cgtgcggcac ccggcgcgcc cgtgtccatg gaacgatgta tgttcctccc ggcctgcagg
      301 ggaacgcgca atggggagag gtgtggctcg ttcacgattc gcccgaactg gaatacgacc
      361 tgaaggagcg cgaacgcctg gtgaccctcg cgcttcttgg caaagacgtg gaattcctcc
      421 gcaggcagtt gccggcgctc atccaactgg gagtccttcg cgagaagatc aatatcctga
      481 accgggggca aatcccgact gcaatcaccc tgccgccgcg tcgtgagatt actgccctga
      541 cgccgtttga ttccattttc cagggcattg tcgttgaaag caggccggca actgcaccgg
      601 agttggccgc cggactggac aagctcaagg acgaactccg cgactcagtt gaggcaatcc
      661 accgacgact ccagacatcg atggacagcc tgcgggcggc ccgggcggag caaaagcgaa
      721 tcatgttcgc cggcgccaac ggttccgcca cccccaaacg cgtgatggcc gccccagtcg
      781 ggctcgatgg caggttctcg ttctccgatg taccccctgg aaaataccgg cttcacgcgc
      841 agtacagact ccagcaatgg ttcgtctccg ttccccttga ggtcggccgg agtgacgtcc
      901 agaaggacat acccaggctt ccgggagttc tccttgattc cagggccatc atcaataccg
      961 aggctctgtg cgcaaagatc ctcgctcttt gagggaaatg atcgacagaa ggcgtttggc
     1021 cccactgcgt tgggttatag gatggttcgc gctgacgccc gtcgagatac tggttttggc
     1081 ggcagtctcc gccacggttt tctggcaggc cttgccgcgg tacaaggaat ggacgttccg
     1141 tcagcttgtc gccgaggaga ccggcgacgt cacccagttg aactacgctc tgagcggcga
     1201 cacaggcctt tttgtgaggt gtccggcgcg cctcgatgac tgcccggacg gatcaaccac
     1261 cgaaacgtgc tcgcttttct ctgccctgtc tcccgagttt cgcgtcgctt ctgcatggcg
     1321 caaagagaac ggcgattatt tggggccggc cggcggaaga taccgatatc ttcctggcga
     1381 gtgcagactc gccccggttg ggaaagaatc tcaggggggc aagcggcgtc attgaccaac
     1441 gagcgcaacc ttattgcgta tctgctgaag aaacaacgcg gagtatacgg aatcgtttcg
     1501 ttcctgcggg cacttgaacc gtaacttcct ccccggctcc atgtcccaca agccccctcc
     1561 ccactggtga accggtcgag atcttccccg acgcgaagtc gctttccgcc tcggatacca
     1621 gcgtgtagct gaatcgtttg ccggtattca ggtcctcgag gtccacccgg cgcccgatgt
     1681 gcgccttccc ttcaggcaga ctctgatcgt ccagaacaat cgcgcgggaa agctggtcct
     1741 gaagtttgg
//
LOCUS       DAOTNG010000468         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_54251_length_1722_cov_5.481704, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000468 DAOTNG010000000
VERSION     DAOTNG010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54251_length_1722_cov_5.481704"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1079
                     /locus_tag="P5179_14485"
     CDS             360..1079
                     /locus_tag="P5179_14485"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96474.1"
                     /translation="MRSMCAVLAGAVLGAIVPVAGASPVSITADVSGVSHYFWRGYDL
                     LGGAKLPVQPALTATAENGLSAGIWGSAALLDRSTTDTADELDFTVSYSRPLVPGIEA
                     SAGGTYYYLPNNSVASTAELFASAAFVEIPFEPTLTAYYDTKWLDDSSAEADGWYFLA
                     AASHSVPVGRFSLDLSAGLGFGSNSTFTGPQDLTLRAATTIPVGRVKLTPFLSPILVF
                     EDAVNSDTFGICGGVSVSYAF"
BASE COUNT          336 a          522 c          523 g          341 t
ORIGIN      
        1 gcaagcctcc gcctgcaggg cactccagca tgcttgcccg cgtacgcggg cacatcatgc
       61 gtagaccatt attcttaaca atacttctga caatgtactt cgttttcaag gtaaaagcaa
      121 tttgcgttcc ctcgcgccga aacgccgcca gaaaccagcg cgttcgagac cattccggcg
      181 gggtgtacgg taccgtacac ccgttggtcc atccacgtac cgacgggaac acgtggtccg
      241 cagtttttac ggcgatttaa cgttgacgaa actgacctgc aacgtgcgat gccgataatt
      301 agatgagcgt gcgacgcaaa aacaccagac agacagcgga gagaaggatg acaggcagta
      361 tgaggtccat gtgcgcggtt cttgccggcg cggtgctggg tgccattgta ccggtggcgg
      421 gcgcttcccc ggtaagcatc actgcagacg tgagcggagt aagccactac ttctggcgcg
      481 gatacgatct ccttgggggc gcgaagcttc ccgttcaacc ggccctgaca gcaaccgcgg
      541 agaacgggct atccgcgggc atctggggca gtgccgcact ccttgaccgc agcaccacgg
      601 acaccgcgga tgaactggac ttcacggtga gttactcccg gccactggtt cccggcatcg
      661 aggcctcggc cggtgggaca tattactatc tgccgaacaa ctccgttgcc tcaaccgccg
      721 agttgttcgc cagcgcagcg ttcgtggaga tccccttcga gccaaccctg accgcgtact
      781 acgacacgaa atggctggac gattccagcg cggaggcgga cgggtggtat tttctggccg
      841 cggcctccca ctccgttccg gtgggcagat tttccctgga cctcagcgcg gggctcgggt
      901 ttgggtctaa tagcactttc accggtccgc aggacctcac cctgcgcgcc gcgactacca
      961 tccccgtggg ccgtgtgaag ctcacgccgt tcctgtcacc gattctggtt ttcgaagacg
     1021 cggtaaacag cgatactttc ggaatctgcg gtggagtaag cgtaagctac gccttctgat
     1081 gacgactcgc acgcggtttt gatttgcgtc ccgtacgccc gctggtgcat gtatccacac
     1141 gacgggcgtc cgagatgctg tacaccggtg atgatcgaag ggcccgcttc gtcgggcctt
     1201 ttgatacgaa tggaacaacg gcgtgatcag gttgtgcaca gcggcatttc gacgggcctt
     1261 ctgctccgtt cggtggcctc cgtgggtatg gcggacacgc gggcggtccc cttccggaaa
     1321 gggagtagcg cacgaccact gtgccaagtc gagaacgcgg aggcagcccg cggcgggccg
     1381 tacgtgtgta cgcgaataga tagggagatt cttcaatccg ttcagaatga ccgcttcacc
     1441 gcgaaacgcc ccgatcgggg gccattctga acggcgagtg cgggcggggt tccgcacccc
     1501 gtcagttccg tttttgtgga attcgcaccg cgcaccggca cgcgctcgca gaacgaccgg
     1561 ttttctgtaa gagatcacct gttcgggggt caccccgaat cagcggaggg cacatggaat
     1621 actacgaaga agccaatgcc cgcgtggcgc ttggtttgcg ccgtcctgaa ccgggcgact
     1681 ggtaccacgg gtaccccgaa taccacgttg aacgcgacga cg
//
LOCUS       DAOTNG010000469         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_54872_length_1708_cov_4.591047, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000469 DAOTNG010000000
VERSION     DAOTNG010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54872_length_1708_cov_4.591047"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="P5179_14490"
     CDS             complement(<1..167)
                     /locus_tag="P5179_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRS96475.1"
                     /translation="MMNSIYMTREGRAKLEEELRFLKVEERPRIKRQIVEAREKGDLT
                     ENAEYDAAKEE"
     gene            complement(368..1552)
                     /locus_tag="P5179_14495"
     CDS             complement(368..1552)
                     /locus_tag="P5179_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRS96476.1"
                     /translation="METAKVTISAGPHDRHFVPIRTRVPVPQDARSAVVEPYVASPPK
                     KGYIPAPAVVAQLGEREADGTADVTFILPYLARNAAATFQITFLPSPAEEKAILAPQP
                     NGDIEVTISGTRLGRYHADDKWARPFLHPVMGPFGHSVTRGWPVEPRENESQDHHHHK
                     SLWVAHGSVNGVDNWSEETGHGRQVQKQVLNCQSGPVFGVLETVNDWVDNSGKKVCED
                     RRKWCFWAMPQGLRMFDLEVEFRATEGPVVFGDTKEGGIIAVRVATCMDVPRTGRITN
                     ADGGTNEPETWGKRSPWCDYSGLDEGGNEVGIALFNHPKSFRYPTYWHVRNYGLMTAN
                     PFGLSEFYGDKSRNGSHTLGAGETLVFAYRVYVHAGDVVGGQVADAFHGYINPPSVSV
                     VA"
BASE COUNT          350 a          543 c          450 g          365 t
ORIGIN      
        1 tactcttcct ttgcagcatc gtattccgca ttttcggtga ggtcaccctt ctcccgggct
       61 tccacaatct ggcgcttgat ccggggacgt tcctcgactt tgaggaaacg caactcttct
      121 tccagcttgg ctcgtccttc ccgggtcata tagatgctgt tcatcaaacg aactccctga
      181 ttatgcccac tctgcccgcc tttcccgcgg ctgcccgttc ggaaacgtaa aacaaaacct
      241 gcaccgacgg gcagagtcgg tgcaggaaaa tgtgcacttg agtgtaacgg aaaaggacgt
      301 tcaggtcaag ccttgcgcgc tgaacccctc gtcaccggct tctcttcatt gcctcagctc
      361 gcctctctca ggcaaccacg ctgacggacg ggggattgat gtagccgtga aacgcgtcag
      421 caacctgccc gcccaccacg tcgccagcat gcacatacac ccgatacgcg aacacgagcg
      481 tttcacctgc gcccagcgtg tgggaaccgt tacgggattt gtcgccgtag aactcactca
      541 acccgaacgg attggctgtc atcaggccat agttgcggac gtgccagtag gtcggatacc
      601 gaaagctttt tggatgattg aaaagtgcga ttccaacctc attcccgcct tcgtcgagac
      661 cggagtaatc gcaccagggc gacctcttgc cccacgtctc cggctcgttc gtcccgccat
      721 cagcgttcgt aatacgcccc gttcggggaa catccatgca cgttgcaacc cgaacggcga
      781 tgataccgcc ttccttcgta tcgccgaaca cgacggggcc ttcggtggca cggaactcaa
      841 cttcgagatc aaacatgcgc aggccttgcg gcatcgccca gaaacaccat ttgcgtcggt
      901 cttcgcacac cttcttcccg gagttgtcaa cccagtcgtt caccgtttcg agcacgccga
      961 acaccggtcc gctctggcag ttcagaacct gtttctgaac ctgcctcccg tgccccgtct
     1021 cctccgacca gttatccacg ccgttcacac tgccgtgcgc gacccacaaa gacttgtgat
     1081 ggtggtgatc ctgcgattcg ttttcacgag gttccaccgg ccaccctcgg gtaacggaat
     1141 gcccgaacgg ccccatcacc ggatgcagga aaggcctcgc ccatttgtcg tcagcgtgat
     1201 aacggccgag acgagtgccg gaaatggtaa cctcaatgtc gccattgggt tgcggcgcca
     1261 ggatcgcttt ttcctccgcc ggtgagggca gaaaagtgat ctggaatgtc gcggcggcat
     1321 tcctcgccag ataaggcaga atgaacgtca cgtctgctgt cccgtcggct tctcgttcgc
     1381 caagttgcgc caccacggca ggcgcaggga tgtacccttt tttcggaggg ctcgccacat
     1441 atggttcgac aaccgccgaa cgagcatcct gcgggaccgg gacacgagtt cggattggaa
     1501 caaaatgccg gtcatgcgga ccggccgata tcgtcacttt tgctgtttcc atagagcaag
     1561 ctcctgcacg tgaggcggat tactgaatcg aacaaaaact cggtgtactg actcgagccg
     1621 atgctcagaa tgtaccgagg acaccagagg tgtggcaaac aaccccgcgc ggggtctcgt
     1681 cgcctaggaa tccccgttga acgggcgt
//
LOCUS       DAOTNG010000470         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_55369_length_1698_cov_4.370055, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000470 DAOTNG010000000
VERSION     DAOTNG010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55369_length_1698_cov_4.370055"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..766)
                     /locus_tag="P5179_14500"
     CDS             complement(5..766)
                     /locus_tag="P5179_14500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS96477.1"
                     /translation="MPRHDWWSKEFQKMVVLGESTVEGGWWTASASERWADILANLIN
                     QCQRTPMQYVNKGIGANAISPRSPGYAASRKPSALERYAEDVVAQKPDLFILCYGLND
                     MRAGMPLNDFIDDMRTIVTDVRNACAPLIILTTIYYMTGWKSYPPYDRGSVALTEEFN
                     RAIKALAEEQQCLVADVWDAEGQADWLIHPDGVHANKVGNILIANRVFETIAKNASAL
                     SNSVYERDLPGRVAQELIRIRESQGDPYRAWWQKH"
     gene            complement(785..>1698)
                     /locus_tag="P5179_14505"
     CDS             complement(785..>1698)
                     /locus_tag="P5179_14505"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS96478.1"
                     /translation="RILDGWKTIVEHVCLYEYTNLGWFLPRNFTSLTAKRIRYLSQIG
                     IDGYYAEAHPHPAQMAPVMYLTAKLAWDPALNAEDVLDEWYQRMFREAAPHMKGFYVT
                     LDSAWMHADRRGAWFAGLNRMWEEFAAYPPRYREVAWRHLDAAMRSARDETTRMRVQY
                     VREGHEPAYRVSRAFEWAHSLNTRSSEDEIVRVLSEFERFMETFFRNVESNPDYADIA
                     YRGARFHERTKWIKLDILRCIDRALAGRPEMRARLLATNATFSEMDDTAKNRRTRGYR
                     RWTNRDMETRGVFPSYIADQPPYLPSE"
BASE COUNT          333 a          535 c          444 g          386 t
ORIGIN      
        1 cgtctcagtg cttctgccac cacgcccgat agggatcacc ctgcgactcc cggatccgaa
       61 tgagttcctg cgccaccctt ccgggcaggt ctcgttcata caccgaattg ctcaaggcgc
      121 ttgcgttctt ggctatcgtt tcaaagacgc ggttcgcgat cagaatgttg ccgaccttgt
      181 tcgcgtgaac tccatccgga tggatcaacc aatcggcctg tccctccgcg tcccacacgt
      241 cggcaacgag gcactgctgc tcttccgcca gcgctttgat cgcccggttg aactcctcgg
      301 tgagtgccac cgacccccga tcatacggtg ggtagctctt ccagccggtc atgtagtaaa
      361 tggtcgtgag gatgataagc ggggcgcacg cgttcctgac gtccgtcacg atggtgcgca
      421 tgtcatcaat gaaatcgttc aacggcattc cggcgcgcat gtcgttaagg ccgtaacaca
      481 ggatgaacag gtccggcttc tgggcgacga cgtcctccgc gtaccgttcc agcgcgctcg
      541 gtttgcgcga ggcagcatac ccggggcttc gcggactgat cgcatttgcc cctatccctt
      601 tgttgacata ctgcatcgga gttcgctggc actgattgat caggttcgcc agaatgtccg
      661 cccagcgctc tgaagcggat gccgtccacc acccaccttc cacggtgctt tcgccaagca
      721 caaccatttt ctggaactct ttgctccacc agtcatgacg tggcatatct ccctccaatg
      781 caggtcattc cgacggaaga tacggtggtt ggtccgcgat atatgacgga aaaacgcctc
      841 gcgtctccat gtcgcggttt gtccaccggc ggtaacctcg ggttcgacgg ttctttgcag
      901 tgtcatccat ctcgctgaac gtcgcgtttg tcgcgagaag ccgtgcacgc atctcggggc
      961 gacccgccag cgctcgatca atacaccgaa gtatgtccag cttgatccat ttcgtgcgct
     1021 catgaaaccg tgcaccacgg taggcaatgt ccgcgtaatc gggattggac tcgacgttcc
     1081 ggaaaaacgt ctccatgaac ctttcgaact cggagagcac acggacgatc tcatcttcag
     1141 aacttcgcgt gttcaacgaa tgcgcccatt caaacgcccg ggaaacacgg tatgcaggtt
     1201 cgtgcccctc gcgaacgtat tgcaccctca tgcgggtcgt ctcatccctc gccgagcgca
     1261 ttgctgcatc taggtgccgc catgccactt ccctgtaccg gggaggatat gcggcaaatt
     1321 cctcccacat acgattgaga cccgcaaacc atgctccgcg tcgatccgcg tgcatccacg
     1381 cgctgtcaag ggtaacatag aagcctttca tgtgtggagc cgcttcacgg aacatcctct
     1441 ggtaccactc gtccagaaca tcttcggcat tcaacgccgg gtcccaggcg agcttcgccg
     1501 tcaggtacat taccggcgcc atttgtgcgg gatgcggatg cgcctcggcg tagtatccat
     1561 ctattccgat ttggctcaga taacgaatcc tcttcgcagt gaggctcgtg aaattccgcg
     1621 gaaggaacca gccaagattc gtgtactcgt aaaggcagac atgctcgact atggtcttcc
     1681 acccgtccag tatgcgtt
//
LOCUS       DAOTNG010000471         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_55966_length_1686_cov_5.038627, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000471 DAOTNG010000000
VERSION     DAOTNG010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55966_length_1686_cov_5.038627"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5179_14510"
     CDS             <1..573
                     /locus_tag="P5179_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96479.1"
                     /translation="SRWTQLIVFPKGERFFVLMDRIDSVNDSPEMFLRNDTPGCVRHV
                     QGDTFGEMYLSYLSGPNGVRIPSSEFFNSFPPDLKFGYRRDLNRVPERFIRAYHLRDP
                     KTGAAGPWLAGLTLEPSVVYEAWCSQRPGDIIVMIEEVHGRPVKAGESFSAAHIVGFF
                     DTIEEMNSVYDRYKGHTALTVDEKGWRLGR"
     gene            659..>1686
                     /locus_tag="P5179_14515"
     CDS             659..>1686
                     /locus_tag="P5179_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96480.1"
                     /translation="MAYPHKEVDRVRALAEKVAAIAHEPGMKAIKKRWADVNALRTPD
                     RAPVWCRPVGCWKEIITDDMLECAEPRLRNLEYQFLQTLHKRDINDDSPVGEYFSVNA
                     VFKCDPANTWGVNIHRHLPDVAGGSWRYDPPIKTAADLDRLRIPTYTYCENETNARME
                     QMDDLLGDILPVRLAVGPPLGATLGTAAADLRGLAQMMMDMAVEPELMHRLMGYLRDA
                     TLAAVNQFEATGLITPNNNGPMTCSDPIGETAPDGTLSCKNLWCMANSQEFDQVSPAM
                     WEEFCLNYQMPIFERFGLVGYGCCESLTHKIDGVLSIPNLRIFVCSAWTDLDLVLERV
                     GRTCCIMWR"
BASE COUNT          345 a          510 c          514 g          317 t
ORIGIN      
        1 tcgcggtgga cgcagcttat cgtgttcccg aagggcgagc ggttctttgt gctgatggac
       61 aggatcgaca gcgtcaacga ctcgcccgaa atgttcctgc gcaacgacac gccgggctgc
      121 gtacgccacg ttcagggcga cacttttggc gagatgtatc tgagctacct cagcggcccg
      181 aacggtgtcc gcatcccgtc aagcgagttc ttcaattcct tccctcctga cctcaaattc
      241 ggctaccggc gcgacctcaa ccgcgttccg gagcggttca tccgggccta ccacctgcgc
      301 gacccgaaaa ccggtgccgc gggcccgtgg ctggccgggc tgacgctgga accatccgta
      361 gtgtacgagg cgtggtgcag ccagcgcccc ggcgacatca tcgtcatgat cgaggaagtc
      421 cacggtcggc cggtgaaggc gggcgaatcc ttcagcgccg cccacatcgt cggcttcttc
      481 gacaccatcg aggaaatgaa ctccgtctac gaccgttaca agggtcacac ggcgctgacg
      541 gtggacgaga aggggtggag gctggggaga tagcgatcgt gacgaacaac ccggagtttt
      601 gtcccttctg atttgtagcc acgtccaaac cgtgccaatc ggcagaaagg ataagtggat
      661 ggcgtatcca cacaaggagg ttgaccgcgt tcgtgcgctc gcggaaaaag tcgccgctat
      721 tgcgcacgaa cccggaatga aggccatcaa aaaacgctgg gcggacgtca acgccctccg
      781 cacgccggat cgggcgccgg tgtggtgcag acccgtggga tgctggaagg agatcatcac
      841 ggacgacatg ctcgagtgcg ccgaaccgcg gctgcggaac cttgaatacc agtttctgca
      901 gactctccac aagcgggata tcaacgacga ttccccggtt ggggaatatt tcagcgtgaa
      961 cgcggtattc aagtgcgatc ccgcgaacac gtggggtgtt aacatccacc ggcatctgcc
     1021 ggatgtcgca ggcggctcgt ggcgatacga cccgccgatc aaaactgccg ccgatctcga
     1081 cagactgcgt atccccacgt acacctattg cgagaacgaa accaacgcgc gcatggaaca
     1141 aatggacgac cttcttggtg acatcctgcc ggtcaggctg gcggtgggac ctcccctggg
     1201 ggctacgctg ggtaccgccg cggcggacct gcgtggcctg gcacagatga tgatggatat
     1261 ggccgtggag ccagagctga tgcaccggct catggggtat ctgcgcgatg cgactctggc
     1321 ggccgtgaac cagttcgagg cgacgggcct gatcaccccc aacaacaatg gccccatgac
     1381 ctgcagcgac ccgattggtg aaaccgcacc tgacggcacc ctttcctgca aaaatctctg
     1441 gtgtatggcc aactcgcagg aattcgacca ggtatctccc gccatgtggg aagagttctg
     1501 cctgaactac cagatgccga tttttgaacg tttcgggctc gtgggatacg gctgttgtga
     1561 aagcctcact cacaagattg acggggtcct ctccatcccg aatctccgca tcttcgtgtg
     1621 cagcgcatgg acggacctcg acctcgtgct tgagcgggtt ggccggacct gctgcatcat
     1681 gtggcg
//
LOCUS       DAOTNG010000472         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_59470_length_1618_cov_4.408189, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000472 DAOTNG010000000
VERSION     DAOTNG010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59470_length_1618_cov_4.408189"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..462
                     /locus_tag="P5179_14520"
     CDS             136..462
                     /locus_tag="P5179_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRS96481.1"
                     /translation="MAAIRTTAPAQLKEWLENGKDFVLVDVREQNEWEICHIKGALFR
                     PLSQISSWIDALDPNKEYVFHCHHGGRSHQACALAIARGVENVISLEGGIDSWCTAVE
                     PQMKRY"
     gene            500..>1618
                     /locus_tag="P5179_14525"
     CDS             500..>1618
                     /locus_tag="P5179_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRS96482.1"
                     /translation="MRTALAQITGDDFVTEVPAEFASLDGVRPRYLVKPGNPQEIAAV
                     LEAAGREQWAVLPIGSGSAIAIGNPPRRGDIWLSTARLNAFVEYESADLTATVQSGCL
                     LSDLNRVFGEHGQILPLDPPGAGSRTIGGIVATAQTGTLRLGYGQPRDWILGLQIATP
                     DGKLIRSGGKVVKNVAGYDLTRLFTGSYGTLGIITQVSLKVYPKPATEMTMVCGSDEI
                     VRVWKTAENMLSGNINPVSLEIVSENALRRVGLEVELPGYCLCVRVAGEEADVRVQTM
                     QMERIASESELSVRAPVPAETAGAFWQMVSDLPLGFPIVVRLNTVPSRLPRFLSMAAD
                     TLRQAAECVYFSASAGIGCATLMVEGPSDATLMNTLLPA"
BASE COUNT          349 a          461 c          483 g          325 t
ORIGIN      
        1 cgatgctcag aatgtaccga ggacaccaaa gggtggcaaa caaccccgcg cgggatgtcg
       61 ccgcctagga atccccgttg aacgggcgta tctttggtta ttgttttcga tcaaatactt
      121 aggacgagga aaagcatggc agcgatcagg acaactgcgc cggctcaact gaaggaatgg
      181 ctcgaaaacg gaaaggactt cgttctcgta gacgtccgcg agcagaacga gtgggagatt
      241 tgccacatca aaggagcgct cttccgccct ttgagccaga tttcgagttg gatagacgct
      301 cttgacccca acaaggagta cgtcttccat tgccaccacg gaggacgtag tcatcaggcc
      361 tgcgccctgg caatcgcgcg cggtgttgaa aacgtcatca gcctggaggg tggcatcgac
      421 agctggtgca cggctgtcga accacaaatg aaacgctact gacgacactg acggcatcac
      481 tggattgacg gtaccgggaa tgagaactgc tcttgcgcag ataaccggcg acgattttgt
      541 cacggaagtg ccggccgaat tcgcatcact ggatggcgtt cggcccagat acctcgtcaa
      601 accgggcaat cctcaagaaa tcgctgccgt gctggaagct gccgggcggg aacagtgggc
      661 tgtgcttccc atcggctccg gctctgcaat cgcgatcggc aacccacccc gcaggggaga
      721 catctggctt tccactgccc gcctcaacgc gttcgtggag tacgagtcag ccgatctcac
      781 cgccaccgtt cagagcggat gcctcctctc cgacctgaac agggtgtttg gcgagcacgg
      841 tcagatcctg cctctcgatc cccccggtgc gggcagccgc acaatcggag gaatagttgc
      901 caccgcacaa acagggacgc tgcgccttgg ttacggccaa ccgcgggatt ggatccttgg
      961 cctccagatc gccacgccgg atgggaagct catccgaagc ggcgggaagg ttgtcaagaa
     1021 tgttgcgggc tacgatctca ctcgtctctt caccggttct tacggcacgc tgggaatcat
     1081 cactcaggtc agtctcaagg tttacccgaa acctgccaca gaaatgacaa tggtgtgtgg
     1141 gtcagacgag atcgttcggg tgtggaagac ggcggaaaac atgttgtccg ggaacatcaa
     1201 tccggtttca ctggaaatcg tgtcggagaa cgcgctcaga agggtgggtt tggaagtaga
     1261 actgccaggt tattgcctct gtgtccgggt cgcaggcgaa gaggccgatg tgcgtgtgca
     1321 aacgatgcag atggaaagaa tcgcctcgga atcggaattg agcgtgcgtg cacctgtccc
     1381 cgcggaaaca gcaggcgctt tctggcagat ggtctctgac cttcctttgg gatttccgat
     1441 tgttgttcgt ctgaacaccg tcccgtcgcg gctcccgcgt tttctctcga tggctgccga
     1501 cactctgcga caggccgccg aatgcgtata cttctcggca tccgccggca tcgggtgcgc
     1561 gacgctgatg gtagaaggcc ccagcgatgc gaccctgatg aacacgcttc tcccggcg
//
LOCUS       DAOTNG010000473         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_59759_length_1613_cov_4.415918, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000473 DAOTNG010000000
VERSION     DAOTNG010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59759_length_1613_cov_4.415918"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1613
                     /locus_tag="P5179_14530"
     CDS             145..>1613
                     /locus_tag="P5179_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRS96483.1"
                     /translation="MGSTQRTRPNIVYILADDLGYGDVSCLNPEGKIPTPNIDRIAAQ
                     GMRFTDAHATSSVCSPSRYSILTGRYNWRSSLQKGIVGVYGDPLIAADRLTVAGLLRQ
                     NGYATACIGKWHLGQGWDFDLTEDFLPSNRNGDFPEATPEQKARWREAFSRPTTGGPT
                     TRGFDSYFGVDVPNWPPYCYIENDRTVGIPSEFLPARLLTNNQASIPGPARAGWYFEQ
                     LLPEFGRRAEQFVSEQAHADRPFFLYMPLTSPHTPLAVNDEWKGKSGLNLYADFVMET
                     DAIVGRVLQAIEDNGVEDNTIVMFASDNGCAHYIGVKDLEAKGHFPSAHYRGYKSDAW
                     DGGHRIPYIVRWPGITEPGSACDQLVCLSDLLATCADMLETDLPRDAGEDSVSIVPLL
                     RGENHPVRRYVVHHSIEGKFAIRDGRWKLVLCPGSGGWTEPNDRAAAEKGLPPVQLYD
                     MDADPGEQNNLQAARPELVAQLVRALEEQVSAGRSTPGPS"
BASE COUNT          322 a          502 c          513 g          276 t
ORIGIN      
        1 gcaggatgcg gtgctcgggg tcggggaacg cgcaagactg ttcgaatgac gacgcaccgg
       61 gaagatgtgc agccgaatga agttatccgg tcggatgccg attccgaaca ggcgcagtgc
      121 gaggtacacg ggagggtcag gggcatgggc tcgacacaaa ggactaggcc aaacatcgtc
      181 tacatcctcg ccgacgacct gggatacggg gatgtttcgt gcctcaaccc ggaagggaaa
      241 atcccaaccc ccaacatcga tcgcatcgcc gcgcagggga tgcggttcac cgatgcgcac
      301 gcgacgtcct cagtgtgcag cccgtcgcgg tacagcatcc tcaccggccg ctacaactgg
      361 cgatcctcgc ttcagaaagg catcgtcggg gtgtacggtg acccgctcat tgcggccgat
      421 cgcctcacgg ttgccggtct gttgcggcag aacgggtatg cgaccgcgtg tattggcaag
      481 tggcatcttg gacagggctg ggatttcgac ctgacggaag atttccttcc ctccaatcgc
      541 aacggcgact tccccgaagc cacgccggag cagaaagcac gctggcggga agcgttttct
      601 cgcccgacca ccggagggcc gaccacccgc ggtttcgact cgtatttcgg cgtggatgtc
      661 cccaactggc ctccgtactg ctacatcgaa aacgatcgca cggtgggaat tccctccgaa
      721 ttcctgcccg cccgccttct tacgaacaac caggccagca tccccgggcc agcgcgtgcc
      781 gggtggtatt tcgaacagct tctgcctgaa ttcggccggc gagcggagca gttcgtgtct
      841 gaacaggcgc acgcagaccg tccgttcttc ctctacatgc cgctcacctc gccccacacg
      901 ccgcttgcgg tgaacgacga atggaaaggc aagagcgggt tgaacctcta cgccgatttc
      961 gtgatggaga ccgacgcaat cgtggggcgc gttctgcagg cgattgaaga caacggcgtt
     1021 gaggacaaca ccatcgtcat gttcgccagc gacaacggct gtgcgcacta catcggcgtg
     1081 aaggatctgg aggcgaaggg ccattttccg agcgcgcact accggggata caaatcggac
     1141 gcgtgggacg gcggccaccg aatcccgtac atcgtccgct ggcccggtat taccgaaccc
     1201 gggagcgcgt gcgatcaact ggtgtgtctg tctgacctgc tggcgacgtg tgcggacatg
     1261 ctggaaaccg atctgccccg tgacgccggg gaggacagcg tcagcatcgt gcccctcctt
     1321 cggggcgaaa accaccccgt gcgccggtac gtagtccacc actcgatcga aggcaaattc
     1381 gccatccgcg acgggaggtg gaagttggtg ctctgcccgg ggtcgggcgg ctggaccgaa
     1441 ccgaatgaca gagccgcggc ggaaaagggc ctcccgccgg ttcagctcta tgacatggat
     1501 gcggaccccg gcgaacaaaa caaccttcag gcggcgcgcc ctgaactggt tgcgcagctt
     1561 gtgagggcac tggaagagca ggtatccgcc gggcggtcca cgccggggcc atc
//
LOCUS       DAOTNG010000474         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_61156_length_1590_cov_2.028664, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000474 DAOTNG010000000
VERSION     DAOTNG010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61156_length_1590_cov_2.028664"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="P5179_14535"
     CDS             <1..1330
                     /locus_tag="P5179_14535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS96484.1"
                     /translation="MPPVAEPAIVPPAPKRKQEGKWFLNALSVILLGYAVFGTWFAYL
                     GFPPVYVSEMVLALGVLTVFRYPGMLGAMVTAPLFPLLAAFVVWNAACMIPYLPEYGL
                     DSLRDGAMWGYSAFALIVYGVLTRTPDATGRFIGRFRGFSVVYGALFTFGMVLLFSGS
                     PFRFWLARTSDTGAFVGGVLSHYLLAGASRLVPVFALAPAFYGLSASRSAGLATVGAL
                     ALATALGKRKGRALLVNAALATGLVAFTVAGTAATSALGSGRNVLSNFASVFAAAKEN
                     PLESESNDGTARWRLIWWTKIWDNTVTGEYFWMGRGYGRNIAVEDGFESEKLERGDKP
                     LRSPHSGHLNYLARSGVPGAILWVTLQITWAGQVLFRVLRSRAKGLDEWSGRFAFLLC
                     FWLALVTNAGFGVFLENPVGGIWLWTVFGVGLASMHAFDAEMREKRVART"
     gene            1327..>1590
                     /locus_tag="P5179_14540"
     CDS             1327..>1590
                     /locus_tag="P5179_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRS96485.1"
                     /translation="MNVVMLHNYYQQRGGEDVAVEQDVALLRAHGHTVHFHTIHNEEI
                     ARYGAFQKASLWFRPTCSMRSCREIRAILKTFRPDIMHVHNFFP"
BASE COUNT          270 a          445 c          501 g          374 t
ORIGIN      
        1 gatgccgccc gtagccgaac ccgccattgt tccgcccgcg ccgaaacgca aacaggaagg
       61 gaaatggttt ctcaacgcac tgtcggtaat cctgctgggg tacgcggtgt ttggaacctg
      121 gttcgcctac ctgggtttcc cgccggtgta tgtgagcgaa atggtcctcg cgcttggcgt
      181 tctgacggta ttccgttacc ccggtatgct gggggcgatg gtgacggcgc ctctgttccc
      241 tcttcttgcg gcattcgtgg tgtggaacgc cgcgtgcatg atcccctacc tcccggaata
      301 cggtcttgat tctctgcggg acggggctat gtggggctac tcagcgtttg cgttgatcgt
      361 gtacggggtc ctcacgagaa ccccggatgc aacggggagg ttcatcgggc ggtttcgcgg
      421 gttcagtgta gtgtatgggg cgctgtttac cttcggaatg gttctcctct tttccggctc
      481 ccctttccga ttttggttgg cccggacgtc ggatacgggc gcattcgtcg gtggtgttct
      541 gagtcactac cttttggctg gtgcttcccg ccttgtgccc gtgttcgccc tcgcgccagc
      601 attctacggc ctttccgcga gcagatctgc gggcctcgca acggtcggcg ccctggcgct
      661 ggcaaccgcg cttggaaaac gcaagggacg cgctcttctg gtcaatgctg ccctggcaac
      721 cggcttggtc gccttcaccg ttgccggaac tgcagcgacc agcgcgctcg gttccggtcg
      781 gaatgtcctt tcgaacttcg cgtccgtctt tgcagcggcg aaggagaatc cgctggaaag
      841 cgagtcgaac gacggaactg cacgctggcg cctcatctgg tggacaaaaa tatgggacaa
      901 cactgtgacc ggcgaatact tctggatggg aagaggctac ggacggaata tcgcagtgga
      961 ggacggcttc gaatccgaga agcttgagcg gggggacaag cctcttcgca gcccgcacag
     1021 cggccacctc aactacctcg cccgatccgg cgtgccgggc gctatccttt gggtgacctt
     1081 gcagatcacc tgggctggac aggttctttt ccgggttttg cggagcaggg caaaagggct
     1141 cgacgaatgg agtggtcgtt ttgctttcct actgtgtttt tggctggcgt tagtgactaa
     1201 tgcgggattc ggtgtgtttt tggagaaccc cgtgggcggc atctggttgt ggacggtatt
     1261 cggtgtgggg ttggcgagta tgcatgcgtt tgacgccgaa atgcgcgaaa aacgggtggc
     1321 tcgcacatga acgtcgtcat gctccacaac tactaccagc aacggggcgg ggaagatgtc
     1381 gcggtcgaac aggatgtcgc gctcttgcgg gcacatgggc acacggtcca cttccacacc
     1441 atccacaacg aggaaattgc ccgctatggc gcttttcaga aggcatcact gtggttccgg
     1501 ccgacgtgtt cgatgagatc gtgtcgtgaa atccgggcaa tcctcaagac atttcgccct
     1561 gacatcatgc acgtgcacaa cttctttccg
//
LOCUS       DAOTNG010000475         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_61243_length_1588_cov_4.093933, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000475 DAOTNG010000000
VERSION     DAOTNG010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61243_length_1588_cov_4.093933"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1203)
                     /locus_tag="P5179_14545"
     CDS             complement(166..1203)
                     /locus_tag="P5179_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HRS96486.1"
                     /translation="MAGLRRRGEEVRAFILANLSDHESDIAAFTAKHFGISRTAVIKH
                     LARLVEQGAVLVEGERRGRRYRLHSVTKETFRYPIHDALEEDVIWRQDLRPRFDGLPR
                     NVLEIWQYGVTEMLNNAIEHSEGSEIVIVHVPKGTRTAVMISDDGVGIFRKIQREFNL
                     LDERHALLELSKGKLTTDPVGHSGEGIFFTSRIFDFFGILSGDVWFSHNCDEPLDWIF
                     DTRQRASGTSVFMELENHSTRTTKDVFDEYASDEDYRFDKTVVSARLARYGDESLVSR
                     SQARRVLARVEKFRTVVLDFEGVSIIGQAFADEVFRVFKRANPEVQIIPINTNPDVAR
                     MICRASAHAAT"
     gene            1402..>1588
                     /locus_tag="P5179_14550"
     CDS             1402..>1588
                     /locus_tag="P5179_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96487.1"
                     /translation="MSHVVTIPPVRLSERFERYRAFSPAVPIWCITPDGLDRCIHRFY
                     DTSPVSPSGRYVALTRLP"
BASE COUNT          365 a          438 c          439 g          346 t
ORIGIN      
        1 ttgtgagggc actggaagag caggtatccg ccgggcggtc cacgccgggg ccatcacgga
       61 caaacgatgt tccggtggat atctggaagg agacagttcc aggcttttgg gaggcggatg
      121 cgtagtaagc tcaaatcggc gcggtgggtt gcacacagcg aacaatcaag ttgctgcgtg
      181 cgccgacgct cgacagatca tccgggcaac atcgggattc gtattgattg ggataatctg
      241 gacctctggg ttcgcacgct tgaagacgcg gaacacctcg tcggcgaaag cctgacctat
      301 gatgctgacg ccttcgaaat ccaacaccac ggtgcggaac ttttcaacgc gcgcgagaac
      361 acgacgggcc tgagagcggg aaaccagcga ttcgtccccg taccgcgcca atcgagccga
      421 cacaacggtc ttatcaaaac ggtaatcctc gtcggatgca tactcgtcaa aaacgtcttt
      481 tgtcgtccgc gtggagtgat tttccaattc catgaataca ctcgtgccgg aagcacgctg
      541 ccgggtatcg aatatccagt cgaggggctc atcgcaattg tgagaaaacc aaacgtctcc
      601 cgacaaaatg ccgaaaaaat cgaaaatcct tgacgtaaaa aaaatgcctt cgcccgaatg
      661 ccccacgggg tcagtcgtta acttgccttt ggacagctcc agaagcgcgt ggcgctcatc
      721 caacagattg aattcacgtt ggattttgcg aaagatgccc accccatcat cgctgatcat
      781 aacggcagtt cgtgtgcctt tcggtacgtg gactatgaca atttcagaac cttcggaatg
      841 ttcgatggcg ttgttcagca tctcggttac gccatattgc cagatttcga gaacattgcg
      901 cggaagtcca tcgaaccgcg gtcgcaggtc ttgcctccag atgacgtcct cctccaacgc
      961 atcgtgaatt ggataccgaa acgtttcttt tgtcacggaa tggaggcgat accgacgtcc
     1021 acgtcgctca ccttccacaa gaactgcgcc ctgttcgacg agtcgggcga gatgcttgat
     1081 cactgcggtg cgggaaatac cgaaatgctt cgctgtgaat gccgcgatat cggattcatg
     1141 gtcactgaga ttcgccagga taaaggcgcg gacttcttca ccgcgccgtc tcaaacctgc
     1201 cattggaacc tccatatgtt gaatcataac cttggcgaag gttattataa actttacgga
     1261 ggtatgcggt caagtcttca gtggttagaa gaacccactg gcgttgtaga ttccgaactc
     1321 ggtgtttcac gggatatgct tgcgcgacgt tgacgccgcc gcggcatggg tggccggatg
     1381 cgcgaactca ggagggaggc catgtcacac gtagtgacta ttccgccggt gcggttatcg
     1441 gaacgtttcg aaaggtaccg ggcattcagc cctgccgtgc ccatctggtg catcacgccg
     1501 gacggcctcg accggtgcat ccaccggttc tacgacacga gcccggtcag cccgtccggc
     1561 aggtatgtag cgcttacccg cctgccga
//
LOCUS       DAOTNG010000476         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_61301_length_1587_cov_3.983681, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000476 DAOTNG010000000
VERSION     DAOTNG010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61301_length_1587_cov_3.983681"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="P5179_14555"
     CDS             <1..1282
                     /locus_tag="P5179_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96488.1"
                     /translation="GAWALDQFMEHETTAGRVRSARRFVREEKIALAVSVAMVVLALA
                     VMVLHRSPGWMLKAGEENYPAQIQEALAQVRSGLLARDVLIALVLAALTAGGLILVSG
                     RRMSGTIFAVILTVATIADLWRLDFTFLDIRRPTFAEAPIQQPAEVDIIRNDLHAPIP
                     DSVRLWHHIATGDTLFRIAPVAGLDQAGAMNIESTNEYGLWSLPSVSGYHAAKLRIYD
                     DLTISGGLSRRQVLNMLNARYVIAPPGLPDSTLVPVGGGERTVYRNTTALPRAWWVRS
                     VRAVNDRKAALSSVLAPDFDPAEEAIVLGPAPQILPDSTLAPPRVSLWDFHRIEIETN
                     NSRPAFLVLSEIFYPRGWRATADGKPVEIVQTNFVLRGLEVPAGTKKIELTFDSAAYR
                     WGRRLSFTLFPLFLLLVAEETWRAGRKKKGNPTG"
     gene            1350..>1587
                     /locus_tag="P5179_14560"
     CDS             1350..>1587
                     /locus_tag="P5179_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96489.1"
                     /translation="MVWETGSYTWGRLIISISGVRGIVGEMLTPETALGMTAAFAEVA
                     PEGEILVASDGRISGDSLRAACLAALSAKGRAAVD"
BASE COUNT          302 a          452 c          526 g          307 t
ORIGIN      
        1 gggcgcatgg gcactcgatc agttcatgga acacgaaacc acggcaggcc gcgtgcgttc
       61 tgcgaggcga ttcgtacggg aggagaagat cgcgctcgcg gtaagcgtcg cgatggtggt
      121 gctggctctg gccgtgatgg ttctgcacag atcgccgggc tggatgttga aagcgggaga
      181 ggagaactac cctgctcaga ttcaggaagc gctggcacag gtccggtcgg gtctgctggc
      241 gagggacgtg ctgatcgccc tggttctggc tgcgctgact gccggaggcc tcattcttgt
      301 cagcgggcgg agaatgagcg gaacgatctt cgcggtgatt cttactgttg ccaccatcgc
      361 cgacctctgg cgcctcgatt tcacgttcct ggatatccgc cgccccacct tcgccgaagc
      421 gccgattcag caaccggctg aagtagacat catccggaac gacctgcacg cgccgattcc
      481 agactcggtg cgtctgtggc accatatcgc cacgggagac accctcttcc gaattgctcc
      541 ggtggccggg cttgatcagg cgggcgccat gaacattgag agcacgaacg agtacggcct
      601 gtggagtctg cccagcgtca gcggatacca tgcggccaag ctccgtatat acgacgattt
      661 gactatttcc gggggccttt cgcggcgtca ggtgctcaac atgctgaacg cccgttacgt
      721 gattgcacca cccggtctgc cggattcgac tctggttccc gtcggcggcg gggaaagaac
      781 cgtttaccgg aacacgaccg cattgccccg ggcgtggtgg gtgcgctcgg tgcgcgcggt
      841 caatgaccgg aaagcagcgc tgtcctcggt tctcgcgccg gattttgatc ccgcggagga
      901 ggcgatcgtg ctggggccgg cgccgcagat attgccagac agcaccctgg ctcctccccg
      961 agtgagcctc tgggatttcc accgcattga gatcgaaacc aacaattccc gacctgcatt
     1021 tctggtgctg agcgagatct tttacccgcg gggttggcgc gcaaccgcgg atggcaaacc
     1081 cgtggaaatt gttcagacca actttgtgct ccggggactg gaagtgcccg cgggtacgaa
     1141 aaaaatcgaa ctgacattcg actccgccgc ataccggtgg ggaagaaggc tctccttcac
     1201 gcttttcccg ctcttccttc tgctggtcgc agaggaaacc tggcgggcag ggcggaaaaa
     1261 gaagggaaac ccgacagggt gattcctgcc agacgaatcg cgcgggagca gttgtgcgga
     1321 cgggcgctgg accaaccggg gaaggccaaa tggtgtggga aacagggagt tacacatggg
     1381 ggaggttgat aataagcatt tccggcgtgc ggggcattgt gggcgaaatg cttactccgg
     1441 agacagcact cggaatgact gcggcgtttg cggaggtggc tcccgaagga gaaatcctcg
     1501 tggcatcgga tggccgcatc tcgggggata gccttcgggc tgcctgcctt gcggcgttga
     1561 gcgcgaaagg acgcgccgcg gtcgatc
//
LOCUS       DAOTNG010000477         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_63162_length_1556_cov_2.271153, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000477 DAOTNG010000000
VERSION     DAOTNG010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63162_length_1556_cov_2.271153"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="P5179_14565"
     CDS             complement(<1..1434)
                     /locus_tag="P5179_14565"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HRS96490.1"
                     /translation="MRDIRTDADSGYATAIDARYKSPINLKLSVDGRFAYVVCEKSGE
                     LICVDLRTRRPIASAHVGGHPFDLAVSRDGTRIFVTCREADSVVVIDSGSFREHGRFA
                     TGFSPHGLAIDTTGSRVFVANMAADNLSIVDSRTGTLLSTIVTGRQPFDVAISPDNRL
                     AAITNQLSDRIPFRATPFSEITIVDVTSGRITARVETPGCVIHQGVTWTPDGKWVLAV
                     TEIPRNLVPETQMYQGWMVTYALMVVEPRRGGRVGRLLLDDANLYYADPFDVVCSPDG
                     ETIYISSSGADVVNVLSWSSVKRTLRIREDRITLNDDSLALYARHLGLSGEYVVARIP
                     CGINPKGMALNPTGDTLYVANRLSDAVGIVDTRLMKCVGEIALGGPTEITELREGERL
                     FNYASISFQKQVSCATCHPENNVDGLLYDIAADGGLGRNIVDNRTMRGIAGTAPFKWS
                     GKNPTIERQEGPRAAQLFFRSHGFDQRG"
BASE COUNT          339 a          455 c          437 g          325 t
ORIGIN      
        1 accccgttgg tcaaaaccat gggaacggaa aaacagttgt gccgccctcg gaccttcctg
       61 ccgttcgata gtggggttct tcccactcca tttgaacggg gccgttccag caatgccgcg
      121 catggtcctg ttgtcgacga tgtttcttcc caatcctccg tctgccgcaa tgtcatacaa
      181 aagcccgtca acgttgttct ctggatggca ggtggcacag cttacctgtt tctgaaaact
      241 tatgctggcg tagttgaaaa gccgctcgcc ctccctcaac tcggtgattt ctgtcgggcc
      301 tcccagtgca atctcaccga cgcatttcat gagtcgggta tctactattc cgacggcgtc
      361 ggacaatcga ttggccacgt agagcgtatc accagtggga ttgagagcca tacctttggg
      421 gttgatacca catggaatac gggcgacaac gtactcaccg ctcagaccaa gatgtcgggc
      481 gtagagtgcc agtgagtcgt cgttcagggt aattcggtct tcgcgaatcc gcaaggtgcg
      541 tttgaccgaa ctccaactga gaacgttcac cacgtccgcg ccagacgaac tgatatagat
      601 ggtctccccg tccggcgagc acacaacgtc aaacgggtca gcgtagtaga gattcgcgtc
      661 gtcgaggagg agccgcccga cacgtcctcc ccggcggggt tcgaccacca tgagggcgta
      721 cgtgaccatc cacccctgat acatctgtgt ttccgggact aggtttcgcg gtatttccgt
      781 aaccgcgaga acccatttcc cgtcgggtgt ccacgtcacg ccctgatgga tcacgcatcc
      841 gggagtttcc acacgggccg taatgcgccc cgaggttacg tcaacgatgg tgatttcgga
      901 aaacggggtt gcccgaaagg gaattcggtc agaaagctga ttcgtaatcg ccgccaagcg
      961 gttatccggc gaaatcgcga cgtcgaaagg ctgccttcct gtaacaatgg tgctcaggag
     1021 agttcccgtg cgagaatcaa cgatgctgag gttgtccgca gccatgttgg caacaaacac
     1081 gcggcttccg gttgtatcga ttgcgagccc gtgaggactg aagcccgtgg cgaaccgacc
     1141 gtgttcacgg aacgatccgg agtctatcac cactacggaa tccgcttcgc ggcaagtgac
     1201 aaaaattcga gttccgtccc gggaaaccgc gaggtcaaaa gggtggccgc ccacgtgggc
     1261 ggaggctatc gggcgccgcg ttctgagatc gacgcagatc agttcgccac ttttctcgca
     1321 cacgacgtac gcgaaacgcc cgtcaacgct caatttgaga ttgatcggcg atttgtaacg
     1381 tgcgtcgatt gcagttgcgt accccgagtc agcatcagtg cgaatgtcac gcacatttgc
     1441 cgcggcgatg cagaccgccg ccgcagataa cacgaacaag gtcacgcgcc atgatgaact
     1501 cgtggatgca cgcaccggag aagcccctcc agaccggttc tcagaatggg gcgcca
//
LOCUS       DAOTNG010000478         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_63358_length_1552_cov_4.552438, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000478 DAOTNG010000000
VERSION     DAOTNG010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63358_length_1552_cov_4.552438"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1552)
                     /locus_tag="P5179_14570"
     CDS             complement(<1..>1552)
                     /locus_tag="P5179_14570"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRS96491.1"
                     /translation="CPSWHGFTLRGLTPALRVDGVDFKPCSARVVAHTDHSTAVDYRF
                     REGLSLACHIEPWQGYGYRFHTVLHNNGGTATVLNNVVLLGTDGEGFRVAFGAADDPV
                     RVMEQGNYWGKVRPLSAPKAVPAENAQGEPNEAPGTTHGSSDFVWVAYNQASRMAFLA
                     GFLSSERWSGRISVETAPGGEVLHWGIGFDGADVRLKPHSETPLEDLLFLVGADPWEL
                     LASYAGEVQTIHNPQFPAVPPVSWCSWYAYRLGVSEERILENARIAAQRLKPLGLGIM
                     ELDLGWEAGNLPSTFEENERFPHGLKWLSEEFAKLGFDMGVWKAPFTISEFDPMAKEH
                     PEYLIQGEDGKPAPYWDWFWEPHGKVFILDLTHPGAQAWLREKMTSLAERGVKYLKSD
                     FIGCVTGEAAKRRHNPDIAAGGGLEAARIGARIIRECLPDALQLNCGGPEMPGTGHWP
                     LLYTVQDTGNTGFIGHSFQRDNYQAAAVHLFKNYRWGILQPSCLCVGAPGTLDDARLR
                     ATIAFLTGG"
BASE COUNT          264 a          504 c          508 g          276 t
ORIGIN      
        1 gcccgccggt gaggaaggcg atggtggcgc ggagccgtgc gtcgtccagc gtgccgggcg
       61 cgccgacgca gaggcacgac ggctggagaa tgccccaccg gtagttcttg aacaggtgaa
      121 ccgccgcagc ctggtagtta tcgcgctgga acgagtggcc tatgaagccc gtattgccgg
      181 tgtcctgaac ggtgtagaga agaggccagt gaccggttcc cggcatctcc gggccgccgc
      241 agttgagctg cagcgcgtcg gggaggcatt ccctgatgat acgggcaccg attcgcgccg
      301 cttcgagccc gccccccgcg gcgatgtccg ggttgtgccg gcgcttggct gcctcgccgg
      361 tgacacagcc gatgaagtcc gatttcagat acttcacgcc gcgttcggcg aggctcgtca
      421 tcttctcccg cagccacgcc tgcgcaccgg ggtgtgtcag gtcgaggatg aacaccttgc
      481 cgtgcggctc ccagaaccag tcccagtacg gcgcgggttt gccatcctcg ccttgaatca
      541 ggtattcggg gtgctccttc gccatggggt cgaactcgct gatggtgaac ggcgctttcc
      601 agacgcccat gtcgaaccca agcttcgcaa attcctcgga gagccacttg agcccgtggg
      661 gaaatcgctc gttctcctcg aaggtactcg gaaggttgcc cgcttcccag ccgaggtcga
      721 gctccatgat tccgagcccg aggggcttga gccgctgggc ggcgatgcgg gcattttcga
      781 ggatgcgctc ctccgacaca ccgaggcggt acgcatacca gctgcaccac gagaccggag
      841 gcaccgcggg gaactgcggg ttgtggattg tctgcacttc cccggcatac gatgcgagga
      901 gttcccacgg gtcggcgccg acgaggaaca gcaggtcctc aagcggtgtc tcgctgtgcg
      961 gtttgagcct gacgtcggcg ccgtcgaaac cgattcccca gtggagcacc tcgcctccgg
     1021 gcgccgtttc gacgctgatc cgccccgacc agcgctccga cgagagaaac ccggcgagaa
     1081 aggccatgcg ggaggcctga ttgtatgcca cccacacgaa atccgacgag ccgtgcgtag
     1141 tgcccggcgc ttcgttcggt tcgccctggg cgttttctgc aggcaccgct tttggcgcgc
     1201 tcaacggccg cactttgccc cagtagttgc cctgctccat cacgcggacc gggtcatccg
     1261 ccgcgccaaa ggcgacacgg aaaccttcgc cgtccgtgcc caggagcacg acgttgttca
     1321 gcacagttgc cgtgccacca ttgttgtgaa gcacggtgtg gaagcggtat ccgtatccct
     1381 gccacggctc gatgtggcac gccagtgaaa gcccttcgcg gaaccggtag tccaccgcgg
     1441 tggagtgatc ggtgtgcgcc actacgcgcg cagaacaggg tttgaagtcc acgccatcca
     1501 cgcggagcgc cggagtcaac ccgcgcaggg tgaaaccgtg ccaggatggg ca
//
LOCUS       DAOTNG010000479         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_67689_length_1483_cov_5.114146, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000479 DAOTNG010000000
VERSION     DAOTNG010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67689_length_1483_cov_5.114146"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="P5179_14575"
     CDS             <1..449
                     /locus_tag="P5179_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRS96492.1"
                     /translation="APGPELALTVRELCRGKGADVAFEVSGAYPALHEAFRVTANPYG
                     TVVAGGFYQGEAKGLFLGEEFHHSSHGMGGATRMVALHERIESPASAWGLRRVLETVW
                     RLLVDGSLPVEKLVTHVYPASRAAEAFRLVDEHPEACLKVVLDMRA"
     gene            497..>1483
                     /locus_tag="P5179_14580"
     CDS             497..>1483
                     /locus_tag="P5179_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96493.1"
                     /translation="MLRTSHHSERIQMTDPATGIRVTQITSYPTPSRALFYAWPSITP
                     DNRRVVIYCQRSGRRTAPYDIFRCDTDGLNLFQLTDWPGIALLSIDGKTVYVLSGEQI
                     LYAVDMETGRSEPLIDVNEHVRPPYSVSTFEFADMGREVLFGLRNSTVGGGTYFRVNL
                     ETGQTSHGEGDVVMIGCFQQSGRIEILKNYQRVEPIAQADGTRVFKNVRPEPMTIWSA
                     TRDGSDERFMARMDMFGHHTILGQTDKLQGTGQPPHRCIWIVESGKEPFKLAEGPYFW
                     HSAASFDGEWIVADTSWPDEGLKLVHVPTGHWRTLCHPHAEQDHAGVHPHPGI"
BASE COUNT          296 a          460 c          454 g          273 t
ORIGIN      
        1 tggcccccgg tccggaactc gcccttaccg tgcgcgagct ctgccggggg aaaggggcgg
       61 acgtcgcgtt cgaggtctcg ggagcgtatc ctgccctgca cgaggcattc cgggtgacag
      121 ccaacccgta tggtaccgtg gttgccggcg gattctatca gggcgaggcg aaagggctct
      181 tcctcgggga ggagtttcac cactcctccc acggaatggg tggcgccacc cgaatggtgg
      241 cgctgcacga acgcatagag tctcccgcct ccgcatgggg gttacgacga gtccttgaga
      301 ccgtgtggcg cctcctcgta gatggctcgc ttcccgttga gaagctcgtg acccacgtct
      361 atcctgcctc acgcgcggcc gaggcattcc ggctggttga cgagcacccc gaagcctgcc
      421 tcaaggttgt tctggacatg cgcgcctgac cgctcaacac tgattgttct ccggccccag
      481 aaaaaggaac acgggaatgc ttcgcacttc ccaccacagc gaacgaatcc agatgaccga
      541 cccggcgacg ggtattcggg tcacccagat cacaagttac ccgacgccaa gcagagcgct
      601 gttctacgcc tggccgagca tcacgccgga caaccggcgc gtggtgatct attgccagcg
      661 atccggcagg cgcaccgccc cgtatgatat cttccggtgc gacacggatg gtttgaatct
      721 tttccaactg accgactggc ccggtatcgc ccttttgtcc atagatggaa aaacagtcta
      781 tgtgctttcg ggcgagcaga ttctctatgc ggttgacatg gagacgggca gatccgaacc
      841 gctcatcgac gtgaatgaac acgtcagacc gccgtacagt gtcagcacat ttgaattcgc
      901 cgatatgggc agggaggtgc tgttcggcct ccgcaactca accgttggtg ggggcaccta
      961 tttccgcgtc aatctggaga cggggcagac ctcccacggc gaaggcgacg tcgtgatgat
     1021 cgggtgcttc cagcagagcg ggcgcatcga aatcctgaag aactaccagc gtgtagagcc
     1081 aatcgcccag gcagacggca cgcgcgtgtt caagaacgtc cggcccgaac cgatgaccat
     1141 ctggagcgca accagggacg gcagcgacga gagattcatg gcgcgcatgg acatgttcgg
     1201 tcaccacacg atcctggggc aaacggacaa gctgcagggc accgggcaac caccccatcg
     1261 ttgcatctgg atagtggaat cagggaagga gcctttcaag ctggccgagg gcccctattt
     1321 ctggcactct gcggcgtcat tcgatggcga gtggattgtt gccgatacct cgtggccgga
     1381 tgaggggttg aaactcgtgc atgtgccgac cggccactgg cggacgctct gccatcccca
     1441 tgcagagcag gaccacgccg gcgtccatcc ccacccgggc atc
//
LOCUS       DAOTNG010000480         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_68654_length_1470_cov_2.727915, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000480 DAOTNG010000000
VERSION     DAOTNG010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68654_length_1470_cov_2.727915"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="P5179_14585"
     CDS             complement(<1..218)
                     /locus_tag="P5179_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96494.1"
                     /translation="MTCLRIVKSLIPYVVLTAVVASCTGRITPEKREHAARLFSAGCG
                     LMAESHFADAQVLFDSARALDPAESCYWV"
     gene            complement(196..>1470)
                     /locus_tag="P5179_14590"
     CDS             complement(196..>1470)
                     /locus_tag="P5179_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HRS96495.1"
                     /translation="DVLYHNNRNGTFSDVTRNAGIADEYYGTSACAFDYDRDGDLDIF
                     VANYVPYDTSAAVAQQPGTSRYTIVRGVPMSMAPEGYPGEPDLLYRNNGDGTFTEISR
                     IAVFNRTLGRGLGCVALDFNHDGWVDVYVANDAMQNFLYRNNGDGTFTDVAGETGTAF
                     GEGGIPAGSMGVNSADFNGDGWMDIIVANYEDQTPSVYFNNEGRFFIDRAVPCGIGGP
                     SLIPLQWGVITSDLNNDGHVDAYFAGGHVSSRLEARYPHSRFEQRNKLFLNRGDGNFD
                     DFTEWSGPGLELVRASRGASAADYDNDGDMDIAVVEKSTRFSLLRNDTPHVGNWVRVI
                     LRGERSPRDGTGARVRVLSGGRWKTAEKHSGSSYASTEDPRLLFGLGVSAGADSVEVE
                     WPSGARGLAVGIPANSTVTFREGRGYDVSANR"
BASE COUNT          320 a          461 c          379 g          310 t
ORIGIN      
        1 acccagtaac acgattctgc ggggtcgagc gcccgcgcag aatcgaacag cacctgcgcg
       61 tccgcaaaat gggattcggc catgagacca cacccggcag aaaacaaacg cgcggcatgt
      121 tccctttttt ccggcgtaat cctgcccgtg caagatgcga cgactgccgt aagaactacg
      181 tacggaatca gcgacttaac gattcgcaga cacgtcatac ccgcgccctt cccgaaatgt
      241 gacagtggaa ttggccggta tacccacagc gaggcctcgc gcccctgacg gccattccac
      301 ctcaacggaa tccgcacctg ccgacacacc cagcccgaac agcagtcgag gatcctctgt
      361 actcgcgtaa ctcgatccgg agtgtttctc cgcggttttc cacctgcctc cggacaaaac
      421 cctgacacgg gcaccggtcc cgtctcgagg gctacgttct ccacgcaaga tgacccgcac
      481 ccagttcccc acatgaggtg tatcgttcct cagaagactg aagcgtgtac ttttttcaac
      541 caccgcgatg tccatgtcgc cgtcgttatc gtaatcggca gcggatgcac cacggcttgc
      601 gcgtacgagt tcaagtcctg ggcccgacca ttcggtgaaa tcgtcgaaat tgccgtcgcc
      661 tctgttcagg aacagcttgt tcctctgctc gaaccgtgaa tgcgggtacc gcgcttcgag
      721 gcgcgaggag acatgccctc cggcgaaata cgcatcgacg tgtccatcgt tgttcaaatc
      781 ggaagtaatc actccccatt ggagcgggat gagggagggg ccgccgattc cgcaaggaac
      841 cgctctgtcg atgaaaaagc gtccttcatt gttgaaataa acactggggg tctgatcctc
      901 gtagttcgca acgatgatgt ccatccagcc gtctccgttg aagtcggctg aattgacgcc
      961 catgctgccg gcggggattc ccccttcgcc aaacgccgtg ccggtttcac ccgccacgtc
     1021 ggtgaatgtt ccgtccccgt tgtttcggta gaggaagttc tgcattgcat cgttggctac
     1081 gtagacatcc acccacccat cgtggttgaa atccaatgcg acacaaccca gcccccggcc
     1141 caatgtgcgg ttaaagaccg caattctact gatttcggta aacgtcccat caccattgtt
     1201 gcggtacaaa agatccggtt ctcccggata cccttcggga gccattgaca tcggaacgcc
     1261 gcgcacaatt gtatacctgc tcgtgcccgg ctgttgcgcc acggcggcgg aggtgtcata
     1321 aggaacataa ttcgccacga aaatgtccag gtcgccgtct ctgtcgtaat caaaggcgca
     1381 cgcggacgtg ccgtaatact cgtccgcaat ccctgcattt cgcgtaacat ccgagaaggt
     1441 gccgttcctg ttgttgtggt aaagaacatc
//
LOCUS       DAOTNG010000481         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_70238_length_1448_cov_5.637473, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000481 DAOTNG010000000
VERSION     DAOTNG010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70238_length_1448_cov_5.637473"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="P5179_14595"
     CDS             complement(<1..908)
                     /locus_tag="P5179_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS96496.1"
                     /translation="MRFVSFRTRNDVHFGAVMGSKVVDLCGNPHAAGVLKQANPRSLL
                     ALLQAGPGALESAQRAAEKSLRAGDAVRPLKDLTLAAPVPRPGKLLALAGNYGEHLKE
                     SGHEWAEKAVTTPRLFIKPTTTVTGPRSPIIYPKNGNHIDWEGELGVVIGKTARFVKA
                     KNGMDHIAGFLVINDVSERAVKIGSGRKERDGDKWFDWILGKWFDTFAPMGPAFVTKD
                     EIADVHALHLETRVNGVVKQNASTGQMIFSVAELVEWASRWMTLEPGDIISTGTPSGV
                     GSASGTFLKPGDVVEVEIETLGVIRN"
     gene            1110..>1448
                     /locus_tag="P5179_14600"
     CDS             1110..>1448
                     /locus_tag="P5179_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HRS96497.1"
                     /translation="MAAENSSDSTSRYEQYNRIASDLREAYLADQRPWVVGFSGGKDS
                     TALLEMVWYVMIRLPESSRRKPVYVLASDTRVETPLISKRIGQELRLIQEAAFRDKLP
                     VTTRLVYPKLN"
BASE COUNT          271 a          466 c          423 g          288 t
ORIGIN      
        1 ctgttccgga tcacgcccag cgtttcgatc tccacctcca ccacatcgcc cggcttgagg
       61 aacgtgccgg acgccgaccc gacacccgac ggcgtgcccg tggagatgat gtcacccggc
      121 tccagcgtca tccagcgcga cgcccattcc accagctcgg caacgctgaa gatcatctgg
      181 cccgtgctgg cgttctgctt caccacgccg ttcacccgcg tctccagatg cagcgcgtgg
      241 acatcggcga tctcgtcctt cgtgacgaac gccgggccca tcggcgcaaa cgtatcgaac
      301 cacttcccca gaatccagtc gaaccacttg tcgccgtccc gctccttccg gccggagccg
      361 atcttcaccg cgcgctcgga cacgtcgttg atgacgagga acccggcgat gtgatccatt
      421 ccgttcttcg ctttcacaaa ccgcgccgtt ttgccgatca ccacgcccag ctcgccctcc
      481 cagtcaatgt gattgccgtt cttcgggtag atgatcgggc tgcgggggcc ggttacggtg
      541 gtggtgggtt tgatgaacag gcgcggcgtg gtgaccgcct tctccgccca ttcgtggccg
      601 gattccttca ggtgctcgcc gtagttgccc gccagcgcca gcagcttgcc cggcctcggc
      661 accggcgcgg caagggtgag gtccttcagc gggcgcaccg catccccggc cctgaggctt
      721 ttctccgccg cgcgctgcgc cgattccagc gcgcccggcc ccgcctgcaa cagcgccagc
      781 agggagcgcg gattcgcctg tttcaggacc ccggctgcgt gggggttccc acacagatcc
      841 accaccttgc ttcccatgac agcgccgaaa tggacgtcgt tccgcgtgcg aaatgagacg
      901 aagcgcatgg tattcctcct gagggttgga agggctgttc ttcggctgtg gaatgtacca
      961 tgctcccgcg ggagtacgcc acggatggga ggggggaaac cagatagata tccaatcgtc
     1021 gagatgacgc attgcaggca caccgacttt tggctaactt tgcgtgttat acccgcagaa
     1081 aacaccgcag tcaatctagg aagaagctca tggcggcaga gaattcatcc gatagcactt
     1141 cacgctacga gcagtacaac aggatagcat ccgatttgag ggaagcttac ctggcagacc
     1201 aacgcccatg ggtggttggg tttagtggtg gcaaagactc aaccgcgctt ctggaaatgg
     1261 tttggtatgt gatgatccgg ctgccggaat catcgcgacg gaaaccggtg tacgtgttgg
     1321 catcggacac gcgggttgag acgccgttga tatcgaagcg catcgggcaa gagttgcgac
     1381 tcattcagga agctgcgttt cgcgacaaat tgcctgtcac cacgcgcctt gtttacccca
     1441 aactgaac
//
LOCUS       DAOTNG010000482         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_71892_length_1426_cov_3.660102, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000482 DAOTNG010000000
VERSION     DAOTNG010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71892_length_1426_cov_3.660102"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="P5179_14605"
     CDS             <1..358
                     /locus_tag="P5179_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96498.1"
                     /translation="TEEEIMRVLDEFEMFMTTYFTGVEGDPAYSDTYYRGYRFHERTK
                     WIKLDILRCVDRALANRPEVRQRLLETNATFSEMNDTARNRRTVGYRRWTNKSMSERG
                     IYPNFIPDQPPYLPME"
     gene            379..>1426
                     /locus_tag="P5179_14610"
     CDS             379..>1426
                     /locus_tag="P5179_14610"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRS96499.1"
                     /translation="MPDNAKQFTIAREGKPCATVIVPRAAEPRLLDSVADFVHVIERM
                     AGPTLPVLTDGIQTGNGGEIHIGLTDFVKQSGLLPESLPVNGYRIAVCEEHGQPCLVI
                     AGPTTLGTTHGVAGFLTDDLGVLWGMADPLFEDIPVRRDVAVGALDRTERPTFGFRTL
                     LGPDLTWARRNRVDDGNRTLPYYGHSHNMHAIMPPDQYRDHPEYFALRGGTRIIPDGH
                     FGPQPCMTHPDVIRIGVEAARRYFDENPTARTFSLCPNDTKEFCECPNCAALDEGRPL
                     HRGDKIYSDSYFHWVEAVAKEILKSHPDKHLGTYGYWATVLPPRHIKKLPPNVVVFLT
                     VDSSQYFDPDYEAHD"
BASE COUNT          290 a          481 c          428 g          227 t
ORIGIN      
        1 caccgaagaa gagatcatgc gcgttcttga cgagttcgaa atgttcatga cgacgtactt
       61 caccggtgtt gagggcgacc cggcctactc ggacacctac tatcgcggtt accgtttcca
      121 cgagcgcacg aaatggatca agctcgacat cctgcgttgc gtggaccgcg cgctcgccaa
      181 ccggccggag gtgcgtcaga gacttctgga aaccaacgcc accttcagcg aaatgaacga
      241 taccgcacgg aaccgacgca ccgtggggta ccgccggtgg acgaacaaat ccatgagcga
      301 acgcggcatc tacccgaatt tcatacccga tcagccaccg tatctgccaa tggaataacg
      361 gcacaggaag gacatgtcat gccagacaac gcaaagcagt ttaccatagc gcgcgagggg
      421 aagccatgcg ccaccgtcat cgtgccccgt gccgccgagc cccgcctcct cgattcggtt
      481 gccgatttcg tgcacgtgat cgaacgtatg gccgggccga cgctgccggt gctgaccgac
      541 ggcatccaga cgggcaacgg tggggaaatc catatcggcc tgacggattt cgtgaagcaa
      601 tcggggctcc tgccggaatc cctgccggtc aacggctacc gcatcgcagt gtgcgaggag
      661 cacggtcagc cgtgcctggt gatcgccggg ccgacgacgc tgggcaccac gcacggcgtg
      721 gcaggcttcc tcacggacga cctcggcgtg ctgtggggca tggccgaccc gctgttcgag
      781 gacattccgg tgcgcaggga tgtggcggtt ggtgcgctgg accgcaccga acgcccgaca
      841 ttcggcttcc gcacacttct ggggccggac ctgacctggg cgcggcgcaa tcgcgtggac
      901 gacggcaacc gcacgctgcc gtactacggc cacagccaca acatgcacgc catcatgccg
      961 cccgatcagt accgcgatca ccccgagtac ttcgcgctgc ggggcggcac acggatcatc
     1021 cccgacggac atttcggccc gcagccctgc atgacgcacc ccgacgtcat ccggatcggt
     1081 gtcgaggcgg cgcggcggta cttcgacgag aacccgactg cgcgcacatt cagcctctgc
     1141 ccgaacgaca cgaaagagtt ctgcgagtgc ccgaactgcg ccgcgcttga cgagggccgc
     1201 cccctgcacc gcggggacaa gatctactcc gacagctact ttcactgggt ggaggcggtg
     1261 gcgaaggaga tcctcaagag ccaccccgac aaacacctcg gcacctacgg ctactgggcc
     1321 accgtgttgc cgccgcgcca catcaaaaag ctgccgccaa acgtggtcgt cttcctcacg
     1381 gtggattcca gccagtactt cgaccccgac tacgaggcgc acgatt
//
LOCUS       DAOTNG010000483         1424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_72050_length_1424_cov_4.334551, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000483 DAOTNG010000000
VERSION     DAOTNG010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72050_length_1424_cov_4.334551"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="P5179_14615"
     CDS             complement(<1..986)
                     /locus_tag="P5179_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96500.1"
                     /translation="MVGKDWTVWGVAELSCWILHRPERWTTGHDAEDCIDAHINMGIT
                     HIAWDLGRSVLQYHSDLPDATCYGVGKHYDEFYPQASAQYKADMQACHDRCQLRVALM
                     YGKEKGCTIYGRLCMNRHYHPGSSGKSRFSLLHPEWHEIGKDGWLDTSRMCYGIPEYR
                     RERIAILKEAMEIGVDGLMLDFVRQPPALRYHPALVNRYRELTGKDARFLDPSAKAEV
                     LDWCRFRAGYVTDLLRELKTALDPFRQKWGKRVPVQVRIPNDGVEANMIAGFDVLTWC
                     EEGLVDELSLSELHWLPGYTDWDDKPYIALGKQHGIPVFGSSNCLPVQRGGWG"
BASE COUNT          301 a          445 c          374 g          304 t
ORIGIN      
        1 ccccagccac cccgttgtac gggaaggcaa ttgctgctgc cgaacaccgg aataccgtgc
       61 tgctttccga gagcaatgta gggcttgtcg tcccagtcgg tgtagccggg caaccagtgc
      121 agttcgctca gggaaagctc gtccaccaga ccctcttcac accatgtgag gacgtcgaac
      181 cccgcgatca tgttcgcttc gacgccgtcg ttcggaatgc ggacctgaac cggcacccgt
      241 ttgccccatt tctgccggaa cggatcgagc gccgttttca gctctctcaa caggtctgtc
      301 acgtaccccg cccggaaccg gcaccaatca agcacttctg ccttggctga tggatccagg
      361 aaccgcgcat ccttgccggt aagttcgcgg taacggttca ccagcgcggg gtggtaccgc
      421 agcgccggcg gctggcggac gaaatcgagc atcaggccgt ccacaccgat ctccatcgcc
      481 tctttgagaa tggcaatgcg ttcgcggcgg tattcgggga tcccgtaaca catgcgcgag
      541 gtatccagcc acccgtcctt tccgatctcg tgccattctg gatgaagaag cgagaaacgc
      601 gacttgcccg aacttccggg gtggtagtgc cggttcatgc acaacctgcc gtagatcgtg
      661 cagcccttct ccttgccgta catcaacgcc acccgaagct ggcaccggtc gtgacacgcc
      721 tgcatgtcgg ctttgtactg ggcgctcgct tgggggtaga actcgtcgta gtgcttgccg
      781 accccgtaac acgtcgcgtc cggcagatcg ctgtggtatt gcagcacgct cctgccgaga
      841 tcccatgcga tgtgcgtgat gcccatattg atgtgggcgt cgatgcaatc ctctgcgtcg
      901 tgccctgttg tccatcgctc gggacgatgc agaatccagc aactcagctc cgcaacgccc
      961 cagacggtcc aatccttccc caccatgatt tcgtctccat gtattctgct cgaggaatcg
     1021 gcccgccgca aatccgtcgg ccggtcccgc gtcggaatta tgatgccgtc aacccttcaa
     1081 aaccgcctgc aacgaaaaag ggctcgtgtc aactcacgag cccgaagata tgccgaaggt
     1141 gggaatcgaa cccacacgtc ctcgcggaca ccggattttg agttttgaga agaccacaca
     1201 aggcaaaaaa ccgcaatacg ttacatatct gttttattgc cacttgcaaa aatcagccta
     1261 ttgcaccgat ttgtcgattg tcgcaggttt ttgatttcaa gtgtgccatg agtgtgccat
     1321 gggagtcgac gtaagtgcat atttcttcat tgcacaatcg caaaccgagc gaggaggcca
     1381 tttcactaga ccgcgcaacg ataagcatac aacacaccca caat
//
LOCUS       DAOTNG010000484         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_74054_length_1399_cov_3.249256, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000484 DAOTNG010000000
VERSION     DAOTNG010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74054_length_1399_cov_3.249256"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="P5179_14620"
     CDS             complement(<1..>1399)
                     /locus_tag="P5179_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96501.1"
                     /translation="LMRYGGGNADRGSIPHSIVHEYNLIGDPGLQITPPVNRAVLVPN
                     VRTPALAQQITVSGSLPQPINGTATVALVDDYQFEVAPGMQVPVSGGTFSAVFNAPTS
                     LVGTGLTLKTYVAPAATGSTADWAGYARMAVADILVDSVIVERTQPDSFYISSTVYGT
                     AGIQAVVCSLVVNSAGTYDNVPMLRVGSSSWFRTVRALDITGIPSDDLYAFAQPVIKA
                     VDGNGKVTIRRDIAPIYPNARAALRVVSAELVGASRGELGVTIANRGTIPSDSVIAKA
                     WVFDDDGVRTLLSETRLTPLASQTNPISDAGDVTIAKPTATQGSGDEILPQYARASFW
                     LPDSLLRGQSPGKRILVAAEAADGRAARDSTMFTVPASVGVYSSASQYALRVVVPSGK
                     FSAEVAPGTFAAETLVTVSQTTAPAKTTQPDVDPPGAKAGRLADACEVKWLAPVVPSP
                     GASLALTLHLDKNDTD"
BASE COUNT          277 a          420 c          427 g          275 t
ORIGIN      
        1 cgtcagtatc atttttgtcg aggtgcaatg tgagtgcgag agaggcgccc ggtgacggaa
       61 ctacgggagc aagccatttc acctcgcacg catcagccag ccgtcccgct tttgcgccgg
      121 gcggatccac gtcgggctgg gtggtttttg ccggcgccgt tgtctgggac acggtcacga
      181 gggtctctgc cgcgaacgtc cctggcgcga cttcagccga gaacttgccg gacggaacga
      241 cgacccgcaa cgcgtactgc gatgcggagg aatacacacc cacggatgcc gggaccgtga
      301 acatcgtgga atcccgcgca gctctgccgt ccgccgcttc agcggcgacc aggattcgtt
      361 tccccggact ttgaccgcgc aagagcgaat ctggaagcca gaacgacgct ctcgcgtact
      421 gcggcagtat ttcgtcccca ctcccttgtg tcgcggtcgg tttcgcgatc gtcacgtcgc
      481 cagcgtcaga aattggattc gtttgcgatg cgaggggcgt gagacgtgtt tctgacaaca
      541 gagtgcgaac cccgtcgtca tcaaaaaccc atgcttttgc gattacagag tcggaaggta
      601 ttgttcccct gttggcgatg gtaacaccga gttccccccg cgacgcgccg accaattccg
      661 cggacacaac ccgcaacgcc gcgcgagcgt tcgggtatat cggagcaata tcgcgccgga
      721 tggtaacctt cccgtttccg tcaacggctt tgatcacggg ttgggcgaac gcatacaggt
      781 cgtcagacgg gattccggtg atgtcgaggg cgcgaacagt gcggaaccac gacgatgaac
      841 cgacgcgaag catcgggaca ttgtcgtacg tccccgccga gttcacgacg agagaacaga
      901 ccacggcctg aatgccagcg gtaccgtaga ctgtagaact gatgtagaag gaatccggtt
      961 gcgtcctttc cactatcacg ctgtcgacga ggatgtcggc aactgccatc ctcgcatacc
     1021 ctgcccagtc ggcagtgctt ccggtggctg ccggcgcaac gtacgttttc agcgtgaggc
     1081 cggttcccac gagggaagtt ggcgcgttga aaaccgcact gaacgtccct cccgagacgg
     1141 gaacctgcat gcccggagcc acttcaaact ggtagtcgtc cacgagggct acggtcgcag
     1201 tgccgttgat cggttgcgga agcgacccgc ttaccgtgat ctgttgagca agggcgggtg
     1261 tgcgcacgtt cggaaccagc actgctctgt tgacgggtgg cgtaatctga agccccgggt
     1321 caccgatgag gttatactca tgaacgatcg aatgcggaat cgaacctcga tcggcgtttc
     1381 cgcctccgta ccgcatcag
//
LOCUS       DAOTNG010000485         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_74217_length_1397_cov_3.446349, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000485 DAOTNG010000000
VERSION     DAOTNG010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74217_length_1397_cov_3.446349"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="P5179_14625"
     CDS             <1..391
                     /locus_tag="P5179_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96502.1"
                     /translation="GVLGVVRVYGGDGLWLSRSEAPRGGRYRSLRVEEICTSVRTGVW
                     RPAPGGVLVDTACAILSNVDAAGTPAFEYEALAFGEPLVRGVRVKGLDGQGYVVVANF
                     GDSDVTVEVSGTRMGVGAGSATMQAIE"
     gene            547..>1397
                     /locus_tag="P5179_14630"
     CDS             547..>1397
                     /locus_tag="P5179_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRS96503.1"
                     /translation="MTTRERFHATMNFQPFDRLPILEWAGWWDQTIARWHTEGLPADL
                     TDRYDICRHFGLDIYKQDWFGITKPGFPSPKSHGAGVIETEADYDRVREFLYPDNPVD
                     PRRWEALAAEQATGEVVLWFSLNGFFWFPRTLLGIQRHLLAFYDQKELMHRINTDLLE
                     WNLKVIDQLCAVCTPDWMTFAEDMSYNYGSMLSKQAFDEFMLPYYRQIVPKLKERGII
                     TIIDSDGDITVPAHWFEEAGLEGILPLERQAGVDIAKLRREHPRMRFIGHFDKMTQDK
                     GEDVMRA"
BASE COUNT          252 a          406 c          482 g          257 t
ORIGIN      
        1 cggggtgctt ggcgtggtgc gggtttatgg cggagatggg ctgtggctga gccgctcgga
       61 ggcgccccgc ggcgggagat acaggagcct ccgcgtagag gagatctgca cctcggtgcg
      121 gacgggggtc tggcgtccgg cgcccggtgg ggtgctcgta gacacggcgt gcgcgatcct
      181 ttcgaacgtg gatgcggccg gcacgccggc gtttgaatac gaggcgctcg cgttcgggga
      241 gccactcgtg cgtggagtcc gcgtgaaggg gttggacggg caggggtacg tggttgtcgc
      301 gaatttcggg gacagcgacg tgacagtgga ggtgtccggc acccgcatgg gcgtcggcgc
      361 ggggagtgcg accatgcaag cgatcgagta actctcgcag tttgcgggca atctctggat
      421 tcccgccttc gcgggaatga cggtagagag ggcagccgtc ttcctcctgc cgtgttgccg
      481 cgcagcgtct cccgccggta atttcctttc atcacactct cgctctcact ccggggaaac
      541 acgaacatga ccacacgcga acgcttccac gcgacgatga acttccagcc gttcgaccgt
      601 ctccccatcc tcgaatgggc gggatggtgg gaccagacca tcgcgcggtg gcataccgag
      661 gggctgcccg ccgacctgac ggaccggtac gatatctgcc ggcatttcgg actcgatatc
      721 tacaaacagg actggttcgg catcaccaaa ccggggttcc cctcgccgaa atctcacggg
      781 gccggggtga tcgagaccga ggccgactac gatcgcgtgc gggagttcct gtatcccgat
      841 aacccggtgg atccacggcg ctgggaagct ctggcggcgg agcaggcaac cggggaggtg
      901 gttctctggt tcagcctgaa cggcttcttc tggtttccgc ggacgctgct cggcatccag
      961 cgtcacctgc tggcgttcta cgaccagaag gagctgatgc accgcatcaa caccgacttg
     1021 ctggaatgga acctgaaagt gatcgaccag ctttgcgcgg tctgcacgcc cgactggatg
     1081 actttcgcgg aggacatgag ctacaattac ggctccatgc tctccaaaca ggcgttcgat
     1141 gagttcatgc tgccgtacta ccggcagatt gtgccgaagc tgaaggaacg cgggatcatc
     1201 accatcatcg actccgatgg cgacatcacg gtgccggcgc actggttcga ggaggcgggg
     1261 ctggagggca ttctgccgct ggaacggcag gccggcgtgg atatcgcgaa gctgaggcgc
     1321 gagcatcccc gcatgcggtt catcgggcat ttcgacaaaa tgacgcagga caagggcgaa
     1381 gacgtgatgc gcgcgga
//
LOCUS       DAOTNG010000486         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_76210_length_1374_cov_2.003791, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000486 DAOTNG010000000
VERSION     DAOTNG010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76210_length_1374_cov_2.003791"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="P5179_14635"
     CDS             complement(<1..721)
                     /locus_tag="P5179_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96504.1"
                     /translation="MAKRARFSAQPDSEVRVYPTWNDLELHAVDLHFHVGTARPEENS
                     MLDVVSYAVATGRRVICMTDHWWKFTRIPSKPQPHYAPTMDGYRRFAADANEMRATFP
                     EVCLLFGPEIGFGSFKSEELGQAFEVPGVDMFLGEPAGAPAGMGYGDYLIAGVEDIAR
                     CRDRFKRPGTLVHPIRAAIKRVCGDAGPGPLHPKTPQFPPLDSIADPLGHVEELLDVN
                     IRGLAETCVRLDVPVEINMLDF"
     gene            complement(820..>1374)
                     /locus_tag="P5179_14640"
     CDS             complement(820..>1374)
                     /locus_tag="P5179_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96505.1"
                     /translation="HLHTNADSSTLLEIGRQSRQNVFDVRIGVDQGAADVAGVRVRGR
                     NNDLRINTRGGFPDGQHLVFEETAAGNQANLLHREAVFTPEKFVTDRAATPTNEVTWA
                     GAPPPIRVLEATAGEFVHVQRLFPAVLRIVGGRVTSVALVRGTETVTYPPAPERELVL
                     SVGDTVRITSTDAPRVTAIPIKAR"
BASE COUNT          229 a          446 c          442 g          257 t
ORIGIN      
        1 tgaagtccag catgttgatc tccaccggca cgtcaaggcg gacacaggtc tctgcgaggc
       61 cgcggatgtt gacgtcaagc aattcctcga catgccccaa cgggtcggcg atggaatcga
      121 gcggcgggaa ctgcggcgtt ttcggatgca acggccccgg acccgcgtcc ccgcacacgc
      181 gcttgatagc cgcacggatc ggatgcacga gggttcctgg ccgtttgaat cggtcacggc
      241 accttgcgat gtcctccaca cccgcgatga ggtagtcgcc gtaccccatg ccggcgggag
      301 cgccggctgg ttcgccgagg aacatgtcca cgccgggtac ttcaaacgcc tgtccgagtt
      361 cctcggactt gaagctgcca aaccctatct caggcccgaa gagaaggcat acctcgggga
      421 acgtcgcgcg catttcgttc gcatcggcgg caaatcgccg gtatccgtcc atcgtcggcg
      481 cgtagtgcgg ctggggcttg ctcgggatgc gcgtgaactt ccaccagtgg tctgtcatgc
      541 agatcacgcg gcgccccgtt gctacggcgt agctgaccac gtcgagcatg ctgttttctt
      601 ccggccgcgc ggtgccgacg tgaaagtgga ggtccactgc atgcagttca aggtcattcc
      661 aggtggggta cacacgcacc tcgctatctg gttgtgcgga aaaccgggct cttttggcca
      721 tcggtcattt cgataccgcg aacgccagat gcgcggcgcg ctctgcgtct gccactcgat
      781 gaccgaatcg gcggctggcc gctggcgacc tccggctcct taccgtgcct ttatggggat
      841 ggcggtgacc cgtggcgcgt cagtgctggt gatgcggacg gtgtcgccga cgctcaacac
      901 gagctcacgt tcgggtgccg ggggataggt cacagtctcg gtccctcgca cgagagcgac
      961 gctcgtcacc cggccaccca caattcggag caccgccggg aaaaggcgct ggacatgcac
     1021 gaactcgccg gccgtggcct ctaacacccg aatcggcggc ggcgcgcctg cccatgtcac
     1081 ctcgttcgtc ggggtagcgg cgcggtcagt aacgaacttc tcaggcgtga acactgcctc
     1141 ccggtgaagc agattcgcct ggttccccgc ggctgtctcc tcgaacacaa gatgctgccc
     1201 gtcggggaag ccgccgcggg tgttgatgcg caggtcgttg ttgcggccac ggacacgcac
     1261 cccggcgacg tcagcggcgc cctgatccac gccgatccgc acatcgaaca cgttctgccg
     1321 gctctggcgg ccgatttcga gcagggtact cgagtccgcg ttggtgtgga gatg
//
LOCUS       DAOTNG010000487         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_81020_length_1320_cov_4.966008, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000487 DAOTNG010000000
VERSION     DAOTNG010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81020_length_1320_cov_4.966008"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="P5179_14645"
     CDS             <1..525
                     /locus_tag="P5179_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRS96506.1"
                     /translation="DPFDPVPTLWDTAYFYNVSDRRRLDHRKDVLRYCTAPLKEDVEV
                     VGNPEVVLYAASSAVDTDFFARLVDDDPGGTAMEVCYGMVRARHRNGLGKEDLLTPGE
                     VTRFAIRMGITSCCFRKGHRIRLEICSSDFPNHDRNHNTGRNDLLDAEMVIAEQKVHH
                     SPEHPSALILPVSQ"
     gene            683..>1320
                     /locus_tag="P5179_14650"
     CDS             683..>1320
                     /locus_tag="P5179_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS96507.1"
                     /translation="MPAKPNFLWISFEDTNPFYGCYGDPVARTPNLDRLAAQGCRWTR
                     CFSTAPVCAPARSAVITGMYPISIGTHHMRTTHMNPGTPELPTPYSAVVPHYVKCFTE
                     YLRAAGYFCTNNAKTDYQFDPPVTAWDELGTHAHWRNRPDPDQPFFAVFNPTRTHESG
                     MWPEHCPELRFDPAEMRLPPYFPDTLKVREAMARMYTHIEESDAELGQLLAQL"
BASE COUNT          263 a          438 c          368 g          251 t
ORIGIN      
        1 gacccgtttg atcccgtgcc gacgctgtgg gatactgcct acttctacaa cgtatccgac
       61 cgccgccgcc ttgatcaccg gaaggatgtc ctgcgttatt gcaccgcgcc gctcaaagag
      121 gatgtcgagg tcgtggggaa cccggaggtt gtactgtacg cagcctcctc cgcggttgac
      181 acggacttct ttgcccggct tgtggacgat gaccccggtg gaacagccat ggaggtctgt
      241 tacgggatgg ttcgcgcgag gcaccgcaac ggattgggta aggaagacct cctgactccc
      301 ggcgaagtga cccggtttgc cattcggatg gggattacgt catgctgttt ccgcaagggc
      361 caccgcattc gactcgaaat ctgcagcagc gactttccca atcacgatcg taaccacaac
      421 actggcagaa atgacctcct ggatgcagag atggtgatcg ccgaacagaa ggttcaccac
      481 agcccggaac acccttcggc gctcattctt ccggtgagcc aataaatgga ttccttgaca
      541 caggggcggg aacgctggtc cggtccacca ggcccacacc gtccaccatt ctcttcgtgt
      601 cttcgcgtga gacatttccg aacggacctc agaccgctgg attccgcacc tgtctcttgc
      661 ggacaataga aggaactcac ccatgcccgc caaacccaac ttcctctgga tatccttcga
      721 ggataccaat ccgttctacg gctgttacgg cgacccggtg gcccgcacgc caaacctcga
      781 ccggctcgcg gcgcagggct gccggtggac gcgctgcttc tccactgcgc ccgtgtgcgc
      841 ccctgcgcgg tcagccgtga tcaccggcat gtatccgatc tccatcggca cgcaccacat
      901 gcgcacgacg cacatgaacc cgggaacgcc tgagttgccc acgccctatt ccgccgtggt
      961 gccgcattac gtgaagtgct tcaccgaata cctgcgtgcg gcgggctact tctgcaccaa
     1021 caacgcgaaa accgactacc agttcgatcc gcccgttacg gcgtgggatg aactcggcac
     1081 ccacgcccac tggcggaacc gccccgaccc cgatcagccg tttttcgcgg tgttcaaccc
     1141 gacgcgcacc cacgaaagcg gcatgtggcc ggaacattgc ccggagctca gatttgaccc
     1201 cgcggagatg agactgccgc cctactttcc ggacacgctt aaggtgcgtg aggcgatggc
     1261 ccggatgtac acgcatatcg aggagagcga tgcggagctc gggcagcttc tcgcacaact
//
LOCUS       DAOTNG010000488         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_81586_length_1314_cov_5.533757, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000488 DAOTNG010000000
VERSION     DAOTNG010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81586_length_1314_cov_5.533757"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="P5179_14655"
     CDS             <1..263
                     /locus_tag="P5179_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS96508.1"
                     /translation="RVIQVVSLAMIIPTSLSGAYVHARRGNVLWRSVVALSLPGCVGV
                     LAGVALAHSMPSSWLKEIVFPVFLCLMVIRLGWMSRRQAERG"
     gene            complement(328..1161)
                     /locus_tag="P5179_14660"
     CDS             complement(328..1161)
                     /locus_tag="P5179_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS96509.1"
                     /translation="MIGQHVWLWLVPMGFVLGALGTLIGAGGGFVLVPVLLLMYPRNS
                     PETITSISLAVVFFNALSGSVAYGKMGRIDFRSGVLFAAATIPGAILGAFTTDSIPRR
                     TFNGVFGVLMVAAAAYLILQRDKGPGHVGSGDAHKTVRRIVEKDGTVHTYSFSLRAGM
                     LWSTLVGYISSLLGIGGGIVHVPVLARLLNFPVHIATATSHFVLAIMALTGTVVHIAN
                     GSFAHGGFWRTLYLGIGVLLGAQLGALLSNRVKGVWIIRSLAIALGLVGVRILLMAFE
                     S"
BASE COUNT          283 a          391 c          400 g          240 t
ORIGIN      
        1 tccgcgtgat tcaggtggtg tctctggcca tgatcatccc gacctctttg agtggcgcat
       61 acgtccacgc ccgccgggga aatgtcctct ggcgttccgt agttgcgctc tcccttcccg
      121 gatgcgtggg cgtgctggcg ggagtagcgc tggcccattc aatgccatca tcctggctca
      181 aagagatcgt ctttccggtg ttcctttgcc tcatggtgat tcgtctcggg tggatgtcgc
      241 ggcggcaggc cgagcgtggg taacgggtgg cccgaacgcg ggcgggttct ggagcgataa
      301 ccgtgcgtga gagtgtccgg gcgcgggtca ggactcaaag gccatcagga ggatgcgcac
      361 gcctaccagc ccgagcgcga tagccagact gcgaataatc cacacgccct tcacgcggtt
      421 tgagagcaac gcccccagtt gcgcgcccaa aagaacgccg ataccgaggt agagcgtgcg
      481 ccagaagcca ccgtgtgcaa acgaaccgtt tgcgatatga acgacggttc ccgtcagcgc
      541 catgatcgca aggacgaagt gcgacgttgc cgtggcgatg tggacgggga aattgaggag
      601 gcgcgcgaga accggtacgt ggacgatccc cccgcctatc ccgaggaggc tcgagatata
      661 ccccaccaga gtactccaca acattccggc gcgaagactg aacgagtacg tgtggacggt
      721 accatccttt tcaacaatgc gtcggacggt tttgtgcgcg tctccgcttc ccacgtggcc
      781 gggacctttg tctcgctgga gaatgaggta ggcggccgcc gcaaccatca gcaccccgaa
      841 cacgccgttg aaagtccgcc ggggtatgga gtcggtggtg aaggccccga ggatcgctcc
      901 cggtatggtg gctgccgcga acaggactcc ggacctgaaa tcgattctcc ccatcttccc
      961 gtaggccacc gaacccgaca acgcattgaa aaacacgacc gccagggaga tgcttgtaat
     1021 agtttcgggg ctgttccggg ggtacatcaa aagcaggacg ggaacaagaa cgaaacctcc
     1081 gcccgcgccg atcagcgtcc caagtgcacc cagaacaaag cccattggaa cgagccataa
     1141 ccacacgtgc tgtccgatca tttctcgagc gagctccggg atgcgctggc gtgagaccaa
     1201 tctgtcgcgt cttgacccgc gattgcaatc actcaaacgg aacgaggcaa gacagcaaca
     1261 tttcgaatgt ggatgtccgc cggaaaggga ctgcgtcaaa agatatctac catg
//
LOCUS       DAOTNG010000489         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_81906_length_1311_cov_3.931529, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000489 DAOTNG010000000
VERSION     DAOTNG010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81906_length_1311_cov_3.931529"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5179_14665"
     CDS             complement(<1..504)
                     /locus_tag="P5179_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96510.1"
                     /translation="MNSVNPSHFSLRQEISRKLIHLMGLVLPLGYSQVGRSRGLPLLA
                     LAAVMVVTADVLRLYWPFAGRVYRRIFGGLTRSGENSRMTGASYMMVGQALTAALFPV
                     PVAIVAMTFGAVGDSAAAIAGRLSGKHLWLRGKSIEGSLACLIACFVAGTAWAELPLA
                     CVWTGAVV"
     gene            complement(501..>1311)
                     /locus_tag="P5179_14670"
     CDS             complement(501..>1311)
                     /locus_tag="P5179_14670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRS96511.1"
                     /translation="KDRETLIAELLAAGVAVVPTLFSPWGVQLSERREVRHLPGFGSG
                     AFDVQDEGSQLVALATGAASGMVIVDACAGAGGKSLALGAMMRNEGTIAAFDPSEAKL
                     EELRMRAARAGLRNVQTHRVPATPEEVPHEAVFQRLVGHADVVLVDAPCSGSGTARRN
                     PDIFARITPEGVTRYSQTQRRLLETYSRLVRPRGRLVYATCSLFHEENREVAEGWLRD
                     AAGFISIPVERSIPSEIAPSVCEGAYLSVFPDTHGTDGFFVAAFERKSEPQ"
BASE COUNT          274 a          431 c          358 g          248 t
ORIGIN      
        1 gacaaccgca ccggtccaga cgcacgcaag cggtaattct gcccatgctg tgcctgcgac
       61 gaaacacgca atcagacacg cgagggaccc ttcaatcgat ttcccgcgga gccacaggtg
      121 cttaccggaa agcctgcccg caatcgccgc ggcgctgtca ccgactgccc cgaacgtcat
      181 ggcgacaatc gctacgggaa ccgggaacag tgcggcagtg agtgcctgcc cgaccatcat
      241 gtaacttgcc cccgtcattc tcgagttttc gccggaacga gtcagcccgc cgaatatgcg
      301 acggtagacg cgaccggcga atggccagta cagcctcaac acgtccgcgg tgacaaccat
      361 cacagccgca agagcaagca gaggcagacc acggctcctg ccaacctgtg aatacccgag
      421 cggcagaacc agacccatca ggtggatcag tttgcgcgaa atttcctgtc gaagcgaaaa
      481 gtgcgatgga ttcacgctat tcattgtggt tccgattttc tctcaaacgc cgcaacaaag
      541 aatccgtcgg tgccgtgggt gtcggggaac acgctcaggt atgcgccctc acagacagac
      601 ggagcaatct cagacgggat actgcgttcc acgggtatgg agatgaagcc cgctgcatcc
      661 cgaagccagc cctccgccac ttcccggttt tcctcgtgga aaaggctgca cgtcgcgtac
      721 acgagacgac cacgcgggcg aacgagacgt gagtatgttt ccagtagcct acgttgcgtt
      781 tgcgagtacc gcgtgactcc ttccggagtg attcgcgcaa aaatgtccgg gttccgcctt
      841 gccgttccgc ttccggaaca cggtgcatcc acaaggacca catcggcatg cccgaccagg
      901 cgctggaaca ctgcctcgtg cggtacttcc tcaggagttg ccggaaccct gtgggtctgc
      961 acgttcctca gtccggcacg tgccgctctc attctcagtt cttccagttt tgcctcgctg
     1021 gggtcgaatg ccgcgatcgt gccttcgttc cgcatcattg ctcccaacgc aagggatttc
     1081 cctccggcgc ccgcgcatgc atccactatc accattcccg aagctgcacc ggtggcaagc
     1141 gcgacgagct ggctgccttc atcctgaacg tcaaacgctc cggaaccaaa ccccggcagg
     1201 tgacgcactt ctcttctctc tgaaagctgg acgccccagg ggctgaacag ggtaggtaca
     1261 acagcgactc ccgcggcgag gagctccgcg atcagcgttt ctctgtcttt c
//
LOCUS       DAOTNG010000490         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_82497_length_1305_cov_3.928800, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000490 DAOTNG010000000
VERSION     DAOTNG010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82497_length_1305_cov_3.928800"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="P5179_14675"
     CDS             complement(<1..776)
                     /locus_tag="P5179_14675"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRS96512.1"
                     /translation="MDDIERVARLLRTSRSVLFITGAGISADSGLPTYRGRGGIYDGC
                     TTEDGIPIESALSADTFRKRPEVTWKYIARIESASRGRTPNRAHEVIAEMEPLFDRVC
                     VLTQNVDGFHAAAGSTNVIPIHGNLHDLRCTRCSYRTRVPDYSGLELPPSCPSCGAVI
                     RPGVVLFGEQLPAYEVERLLHELERGFDIVFSVGTTSVFPYIAQPVMRAKARGTPTVE
                     INPETTALTDVFDFHIAERAAVALEELWRRYRAGHPPDTKG"
     gene            complement(845..>1305)
                     /locus_tag="P5179_14680"
     CDS             complement(845..>1305)
                     /locus_tag="P5179_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96513.1"
                     /translation="RSRLWGYMAAQEFTAVSPAMHDEFLLQYQLPILKHFGLVAYGCC
                     EDLTHKIDMLRQIPNLRRIAVSPFADVKACAEQIGRDYVVSYRPSPSDMVGYGLAEDR
                     VRAILTKDLEACRDCHVDITLKDVETVQSDPRRVRRWVALTRKVIDEVWK"
BASE COUNT          241 a          389 c          403 g          272 t
ORIGIN      
        1 cccttcgtgt cgggcgggtg tcccgcccgg tatcgtcgcc agagttcctc aagagcaacc
       61 gcggcccgtt cggcgatatg aaaatcgaaa acgtccgtaa gagcggttgt ttccgggttg
      121 atttccaccg tcggagtccc ccgcgctttt gcccgcatca ccggttgcgc gatgtacgga
      181 aacacactcg tggtgccgac ggaaaagacg atatcgaaac cacgttccag ttcgtggagc
      241 aggcgttcga cctcatatgc gggaagctgc tcgccaaaca ggacgactcc gggtcgaatc
      301 accgcgccgc atgaggggca ggatggcggc agttccaggc cggaatagtc gggcacacgc
      361 gtgcggtacg aacagcgcgt gcagcgcagg tcgtgcaggt tgccgtggat tggaatcacg
      421 ttggtgctgc ccgccgcggc gtggaatccg tccacgttct gcgtcaggac gcacacgcga
      481 tcgaacagcg gttccatctc cgcgatgact tcgtgcgcgc ggttgggggt gcggccacgc
      541 gaggcagact cgatcctcgc gatgtacttc catgtcacct cagggcgttt tcgaaatgtg
      601 tcggcggaga gcgctgattc gatgggtatt ccgtcttcgg tggtacaccc gtcgtagatt
      661 cctccgcggc cacgatatgt tggcaggcct gagtccgcgc ttattccggc tcccgtgatg
      721 aagagaacgg agcgggaggt tcgcagcagg cgagccaccc gttcaatgtc gtccagaacg
      781 gcttggtgtt ctggtaaccc gttgtcgtaa tgctgtcgtt tgtgttcttc ggttctctga
      841 gccactattt ccagacctcg tcaataacct ttcttgtcag agcaacccag cgtcgcacgc
      901 gcctcggatc actctgaacc gtttcgacgt ccttgagcgt gatgtccacg tggcaatcgc
      961 ggcacgcttc gaggtccttc gtcaggatgg cgcgcacccg gtcttccgca agtccgtatc
     1021 ccaccatgtc gctggggctg gggcggtaac tcacgacgta gtcccgaccg atctgctccg
     1081 cgcaggcctt cacatccgca aaaggggaaa ccgcgatgcg gcgcaggttc ggaatctggc
     1141 ggagcatgtc gattttgtgg gtaaggtcct cgcagcagcc gtacgcgacc agcccgaaat
     1201 gtttgaggat gggcaactgg tactgcaaca aaaactcgtc gtgcatcgcc ggggacaccg
     1261 cagtgaactc ttgcgccgcc atgtatcccc agaggcgaga acgct
//
LOCUS       DAOTNG010000491         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_83477_length_1296_cov_3.888799, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000491 DAOTNG010000000
VERSION     DAOTNG010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83477_length_1296_cov_3.888799"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="P5179_14685"
     CDS             complement(<1..506)
                     /locus_tag="P5179_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96514.1"
                     /translation="MKQHEAVIEVMKGNGGYATLGFLYQNVLKVQDCKWNTRTPFASI
                     RRIVQDDRFFFKIRPGLWALKTEKNRLPDDILPQAKQPETKNDEYSHTYYQGLLVEIG
                     NLRRYQTFVPSQDRNKRYLGKKLCDVVAIREMYRFSYEHVVNIAQTVDVMWFNERKMP
                     AGFFEIEHS"
     gene            complement(503..775)
                     /locus_tag="P5179_14690"
     CDS             complement(503..775)
                     /locus_tag="P5179_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96515.1"
                     /translation="MEGYGGIGEKDDFPLVVMESSEFAPNQCVFRNDQFGVWLYQANC
                     IEFMDMLIGRYPDGRFDMIFADPPYFLSNGGITCHAGRIAKEKVLS"
     gene            complement(1011..>1296)
                     /locus_tag="P5179_14695"
     CDS             complement(1011..>1296)
                     /locus_tag="P5179_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96516.1"
                     /translation="GHEHVKSGGFDTLEQFGHQLLTVHLHDNDGSKDAHVLPWEGTTD
                     WERFRHLFHSLDYRGNLLLETDIGNSRFKEHAVFLSQAMARAERLLKPPW"
BASE COUNT          300 a          345 c          317 g          334 t
ORIGIN      
        1 gagtgttcaa tttcaaagaa acccgcaggc atttttctct cattgaacca catcacatct
       61 acagtctgtg ctatgttgac cacatgttcg tagctgaatc tgtacatctc acgaattgct
      121 accacgtcgc agagcttttt gcctaggtaa cgcttgtttc tatcttgact tggaacaaac
      181 gtctgatacc ttctcaaatt gccgatttcg acaagcaaac cctgataata cgtatgggag
      241 tattcgtcat ttttcgtttc tggctgtttt gcttgtggaa gaatgtcatc cggcaatctg
      301 tttttctcgg ttttcaacgc ccacaaaccg gggcgaattt tgaaaaagaa gcggtcatcc
      361 tgaacgatac ggcgaatact tgcaaatgga gttcttgtgt tccatttgca atcctgaact
      421 ttcaaaacgt tttgatatag gaacccgaga gtcgcgtagc caccgttccc tttcataacc
      481 tcaatcactg cttcgtgctg tttcatgaca gtaccttttc ttttgcgatc ctcccggcat
      541 gacaagtgat accgccgtta gagaggaaat agggtgggtc cgcgaagatc atgtcaaacc
      601 tgccatcggg gtatctgccg atgagcatgt ccatgaattc aatacagttt gcttgataga
      661 gccacacacc gaactggtca ttgcgaaaca cgcactggtt cggcgcgaat tcggagctct
      721 ccatcactac caacgggaag tcgtctttct ccccgatccc accgtatcct tccaaagcga
      781 actcccgata cttttcttac cgtttgcgcc cgatgcgaag tttgtcgcac acgccccttg
      841 taaggaaggc aaggtggcct tcacaaaacg cgaagcaaca accgaaaccc tcggaactgg
      901 gtgctgagtg tcctcaacct cgttacagac gatccctctt ggtggagtta cgtaacacaa
      961 caatccttga caagtcaagc ccaaagcagt gagtggcctt tcgcgctgtc tcaccatggt
     1021 ggcttgagaa gccgctccgc ccgggccatt gcctgtgaaa ggaagacggc gtgttccttg
     1081 aaacgggaat tgccgatgtc ggtctcgagc agcagattgc cgcggtagtc cagtgagtgg
     1141 aacaggtgcc ggaagcgctc ccagtcggtg gtgccctccc agggaaggac gtgggcatcc
     1201 tttgagccgt cgttgtcgtg aagatggacg gtcaggagct gatgcccgaa ctgctcaagc
     1261 gtgtcgaagc cgccgctttt gacgtgctcg tgcccg
//
LOCUS       DAOTNG010000492         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_84261_length_1289_cov_3.882496, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000492 DAOTNG010000000
VERSION     DAOTNG010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84261_length_1289_cov_3.882496"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="P5179_14700"
     CDS             complement(<1..881)
                     /locus_tag="P5179_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRS96517.1"
                     /translation="MLSQLSTRLAERGTTLRREVIGGITTFATMAYIVVVNPAILSDA
                     LGKELIGALVVATCVSAAVATLLMGWWADYPIALAPGMGLNAYFAYTVVLQNHIPWQV
                     ALAAVFLSGLLFILLTLVNVQEILVTAIPLGIRNGTAAGIGIFIAFIGLQKCGFVISS
                     PATMVMGGDVTSPPFLLTLGGIALTGGLTMRKVRGAFIIGICATAVAGIALGVSPMPE
                     RLFAIPDLPTGLFGQAILRLPDAFELGLVTIVVTFLFIDLFDTAGTLSGVGSAAGFVD
                     AKGRLPRAKRAFLADGVG"
     gene            complement(900..>1289)
                     /locus_tag="P5179_14705"
     CDS             complement(900..>1289)
                     /locus_tag="P5179_14705"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HRS96518.1"
                     /translation="IPHAGDWREGNVVAEGHAVNAPFLVHYAGRRKGAHSESYSLLSV
                     TPENVRISAVKRAETGAFVVVRVWETTGNPTEAKLVFNMPFSAAWSATMEEQLVAPLQ
                     HEGNGITIPLRGWQISTVLIDTQPRSG"
BASE COUNT          299 a          384 c          359 g          247 t
ORIGIN      
        1 cccacgccat ctgcgaggaa agcgcgtttt gctcgtggca ggcgcccctt tgcgtccacg
       61 aatccggccg cgcttccaac tccgctcaaa gtaccggcgg tgtcgaacag atcgatgaaa
      121 agaaacgtaa caacgatggt aacgaggccc agttcgaacg cgtcgggtag acgaagaata
      181 gcctgtccga aaagtcctgt cggcaaatca ggaatcgcaa aaagacgttc cggcatcggc
      241 gaaacaccca gggcgattcc cgcaacggcg gtggcgcaga ttccgataat gaacgcgcca
      301 cgcacttttc gcatcgtgag accaccggtc agcgcgattc cgccgagcgt gaggagaaac
      361 ggaggagagg tgacatcgcc ccccatcacc attgtggcgg gtgacgagat gacaaagccg
      421 catttctgga gcccgatgaa ggcaatgaag atacctatcc cggcagccgt accgttgcgg
      481 attccaagcg ggatcgccgt gacaagaatt tcctgcacgt tcaccaaggt cagcagaatg
      541 aacagaagtc cgcttaagaa caccgcagcc agagcgacct gccacgggat gtgattctgg
      601 agaacgactg tgtatgcgaa atacgcgttc aaccccatgc cgggggcaag tgcgatgggg
      661 tagtccgccc accatcccat aagcagcgtc gcaaccgcgg cagaaacgca ggtagcgacg
      721 accagcgcgc cgatcaattc cttgcccagt gcgtcgctga ggatcgccgg gttcacgacc
      781 acgatatagg ccatggtcgc aaacgtggtt attccgccaa tcacttccct tcgtaacgta
      841 gtgccacgct ccgcaaggcg ggtactcaat tgcgatagca cgggttgtct ccttccacgt
      901 cagccagaac gaggctgtgt atcgatcagg acggtcgaaa tctgccagcc tcgcaacgga
      961 attgttatcc catttccctc atgttgaagc ggcgcgacca gttgctcttc catcgtggcg
     1021 ctccatgcgg cagagaaggg catattgaaa accagtttcg cctctgtcgg gttgccggtt
     1081 gtttcccaca cgcggacgac gacaaatgca cctgtttctg cccgcttcac tgcgcttatg
     1141 cggacgtttt ccggcgttac cgaaagcagg gagtacgact cggaatgagc ccctttccgc
     1201 ctgcctgcgt agtgaaccaa aaagggtgcg tttactgcgt ggccttcagc cacaacattt
     1261 ccttcacgcc agtctcccgc gtgtggaat
//
LOCUS       DAOTNG010000493         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_87147_length_1261_cov_4.930348, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000493 DAOTNG010000000
VERSION     DAOTNG010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87147_length_1261_cov_4.930348"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="P5179_14710"
     CDS             <1..411
                     /locus_tag="P5179_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRS96519.1"
                     /translation="ETMPWVFPDSPESYSELIRAVDRTRFGVHLDPTNLVCSPQRYAR
                     TGDLIRECFEMLGPHIRSCHAKDIVLSGKLTVHLDETRPGLGNLDYRVFLRELSRLDP
                     DTPLMIEHLPTRQEYEAAALHIRTVAREIGVEGL"
     gene            408..>1261
                     /locus_tag="P5179_14715"
     CDS             408..>1261
                     /locus_tag="P5179_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HRS96520.1"
                     /translation="MKTAQVVFTGCCRAELVEHPAREEALEAHEVIGTTLATIISAGT
                     ELAVYQGLAGGNYPAHPGYAAVFRIDAVGSEVKGFRPGDVAFCMGPHRSFQRTKEEFV
                     VRVPSEVPPEQAVFARMMAVSMSTLTTTAARPPAAVLVTGLGLVGHLAAQIFAACGYD
                     VTAVDPLEVRRTIASGSGIGRVFASVPPEIAGSVSLAVECSGNERAVLDACGVVRKGG
                     EVVLVGVPWKKRTDLAAFDLLHAVFHRYVVLRSGWEWEVPLLPTDFRQNSIYGNIAAA
                     MRWISEGRV"
BASE COUNT          247 a          349 c          401 g          264 t
ORIGIN      
        1 gaaaccatgc cgtgggtgtt ccccgactcc cccgagtcgt attccgaact catccgcgcg
       61 gtggatcgga caaggttcgg tgtgcatctc gacccgacca acctcgtgtg cagcccacag
      121 cggtacgcac gcaccggtga cctgattcgt gaatgcttcg aaatgcttgg gccgcatatc
      181 agaagctgcc acgcgaagga tatcgtcctg agcgggaaac tcacggtcca tctggatgaa
      241 acgaggccgg gactgggaaa tctggattac cgggtatttc tccgtgagct ttccaggctc
      301 gatcctgaca caccacttat gatcgaacac ctgccgacgc gacaggaata cgaagctgcc
      361 gcacttcaca tcaggacggt cgcgcgagaa atcggagtgg aagggttatg aaaaccgcac
      421 aagtcgtgtt taccggctgc tgccgggcgg aactggtgga acatcccgcc agagaagaag
      481 ctctggaggc acacgaggtg atcgggacaa cgcttgccac catcatcagc gccggaacag
      541 agcttgcggt atatcagggg ctcgcgggag ggaactatcc ggctcaccct gggtacgccg
      601 cggtgttccg catcgacgct gtcggttctg aggtgaaagg ttttcgtccg ggcgacgttg
      661 ccttctgtat ggggccacac cgttcatttc agcgaacgaa agaggaattc gtcgtacgtg
      721 tgccatctga ggtgccccct gaacaggctg tgttcgcccg gatgatggca gtgtcaatga
      781 gcacgttgac gacaactgcc gcacgaccgc ccgcagcggt tctggtgacg ggtcttggat
      841 tagtcggtca ccttgccgcg cagattttcg ccgcttgcgg atacgatgta acggcggtgg
      901 atccgctgga agtgcgtcga acgatcgcct ctggctcagg aattggccgc gtgtttgcca
      961 gtgttccgcc cgaaattgca ggttcggtca gtctggcggt ggagtgctcg ggcaacgaac
     1021 gcgcggttct tgatgcctgc ggtgtggttc gcaagggcgg tgaggtggta ctggtcggcg
     1081 tgccctggaa aaagcggact gatctcgccg cgtttgacct tcttcatgcg gtgttccacc
     1141 ggtacgtcgt cttgcggagc ggttgggagt gggaggttcc gctcctgccc accgatttca
     1201 ggcaaaacag catctacgga aacatcgctg ccgccatgcg ctggatttcc gaaggaaggg
     1261 t
//
LOCUS       DAOTNG010000494         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_87504_length_1258_cov_3.110557, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000494 DAOTNG010000000
VERSION     DAOTNG010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87504_length_1258_cov_3.110557"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5179_14720"
     CDS             complement(<1..427)
                     /locus_tag="P5179_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96521.1"
                     /translation="MVEELSGTLTPETVDSRRPITVRSVVGGTALIALLAFVTPYSEY
                     RVHSMEFFQGQIPVGALASLVVVLLPINWFLGRFRPAWRISLPELVFMFIMAFAGIMV
                     YHIGMMGLFLSMISSPDYFAAPENRYAEFILPYLPPAAIP"
     gene            complement(520..>1258)
                     /locus_tag="P5179_14725"
     CDS             complement(520..>1258)
                     /locus_tag="P5179_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS96522.1"
                     /translation="MYDHSYDRTWMAPYIGMPIEAVWDTVERYPKRFVAGYAPDPKSP
                     MAIEKLKTAVKHGVRVSGEHKFRTLFDDPDCIRFYRVCGELGLPVTLHIDVPCLPPDD
                     PTKPVQWWYAGDISNLERALRQCPDTVFLGHAPGFWREVSGDADSDPNAYPKGPVKPG
                     GRLRPLFDKYPNLYADLSAGSALTAISRDPEWGRQFLLDYHTRLLFARDYFDSALMDF
                     LRSLNLPDEPFNAILSGNALRLVPLAA"
BASE COUNT          278 a          365 c          393 g          222 t
ORIGIN      
        1 tggggatcgc cgccggcggg aggtaaggca ggatgaactc agcgtagcgg ttctcagggg
       61 cggcgaagta atccggcgac gaaatcatgc tcaggaaaag gcccatcatg ccgatgtgat
      121 agaccattat tcctgcgaac gccatgataa acatgaacac cagctccggc aacgatatcc
      181 gccacgcggg tcggaatctc ccgaggaacc agttgatcgg taacaacacc acgacgaggg
      241 aggccagcgc cccgacgggg atctggccct ggaagaactc catggagtgg acgcggtact
      301 cagagtacgg agtgacaaag gcgagcaacg cgatgagagc agtgccaccc acgactgagc
      361 gcaccgtaat cggacgtctc gaatcaaccg tctcaggggt aagcgttccg ctgagttcct
      421 ccaccatacg cggctccctc cggcaaggtg ttccctcaac aggctatcct cgcaaggaag
      481 ggctcgcgac gataacctgc ggacacgctc cgccgcggct tacgccgcca gcgggacaag
      541 cctcaatgcg ttccccgaca aaatggcgtt gaaaggctca tccggcaggt tcagcgaacg
      601 caggaaatcc atgagcgcgc tatcgaagta gtcccgcgca aaaagaaggc gtgtgtggta
      661 atcaagaagg aactgccgcc cccattcggg atctctgctg attgccgtga gggcgctgcc
      721 tgcggaaagg tctgcgtaca gattggggta tttgtcgaac aggggacgca acctgccgcc
      781 cggcttgaca gggcctttcg gatatgcgtt ggggtccgaa tcggcatcgc cggacacctc
      841 tcgccagaag cccggggcat ggcccaggaa gacagtgtcg gggcattgcc tgagtgcccg
      901 ctcaaggttg gaaatgtccc ccgcatacca ccactgaaca ggctttgtgg ggtcatcggg
      961 tggcagacag gggacgtcaa tgtggagggt gaccgggaga ccgagttcgc cgcatacgcg
     1021 gtagaagcgt atgcaatcgg ggtcgtcgaa cagcgtgcgg aatttgtgct caccggagac
     1081 ccgcacgccg tgtttcacgg cagttttcaa cttttcgatc gccatcggcg atttcggatc
     1141 cggcgcgtac cccgcgacga accgcttcgg gtaccgttct accgtatccc agaccgcttc
     1201 gatgggcatc ccgatgtaag gtgccatcca cgtgcggtcg taactgtggt cgtacatg
//
LOCUS       DAOTNG010000495         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_93922_length_1203_cov_2.657666, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000495 DAOTNG010000000
VERSION     DAOTNG010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93922_length_1203_cov_2.657666"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="P5179_14730"
     CDS             complement(<1..612)
                     /locus_tag="P5179_14730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS96523.1"
                     /translation="MSDSTITPATRWKVAAAAVAIHLCIGSLYAWSVFIKPLQAAHGW
                     SRPELAAAFSLAFLTGGLFSAFRGAWMECRGPRRCAVMSAFCFGAGIAGAGIAAHIGS
                     LWLLYATYGVLGGLGLGSGYVPPINALLKWFPDRRGLAGGMAVAGFGAAALLTGPAAT
                     ELISVVEVPLTFAILGAAYFVVILLASRVMRFPPPDYRPEGWVP"
     gene            complement(698..>1203)
                     /locus_tag="P5179_14735"
     CDS             complement(698..>1203)
                     /locus_tag="P5179_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS96524.1"
                     /translation="GCCCEAMIAARRFAILNPIHTTNFLPEVDEAACTGCGKCVNACP
                     VEAMTLVSSNDPHRPKARKARLNEELCLGCGVCVRACTDGHLSLKSRPQRVITPLNGT
                     HRAVVMAIERGMLQNLIFDNRVLWSHRAMAAVLGVILRLPPVKQALASRQVKSRYLET
                     VIRRYRG"
BASE COUNT          241 a          408 c          378 g          176 t
ORIGIN      
        1 tggaacccat ccctccgggc ggtagtccgg tggtggaaac cgcatcacgc gcgacgcaag
       61 caggataacc acgaagtacg ccgcaccgag gatcgcgaac gtgagaggca cctccaccac
      121 ggaaatcagc tccgtcgcag cggggcctgt cagcaacgcc gccgcaccga acccggcgac
      181 cgccatcccg cccgccagcc cgcgccggtc ggggaaccac ttcagaagtg cattgatcgg
      241 gggaacgtag ccacttccca gtcccagtcc gccgagaacg ccgtacgtcg cgtacaacag
      301 ccacagcgaa ccaatgtgtg cggcaatccc ggctcccgca attccggccc cgaagcagaa
      361 agccgacatc accgcgcatc gccgtggccc ccggcattcc atccatgcgc cgcggaatgc
      421 cgaaaaaagg ccaccggtga gaaacgcgag cgagaacgcg gcggccagtt ccgggcgcga
      481 ccagccgtgc gcggcctgca acggcttgat gaagacgctc cacgcgtaca gggaaccgat
      541 acagaggtgg atggccacgg cagcggcggc gactttccat cgcgtggccg gggtgatcgt
      601 ggaatcgctc atggtaaggc atccagcggg cattaccggc gcgaaaacgg gcgacaaaag
      661 aatagcgcgg gagcgcgggc tgatcaaagc cggcgggtta cccccggtac cgccgaataa
      721 cggtttccag ataccgggat ttcacctgtc tgctcgccag cgcctgtttc acggggggca
      781 gcctgaggat cacgcccagc accgccgcca tggcacggtg gctccacagg acacggttgt
      841 cgaagatcag gttctgcagc ataccccgtt caatcgccat taccaccgca cggtgcgtgc
      901 cgttcagagg ggtgattacg cgttgcgggc ggcttttcaa cgacagatgg ccgtccgtgc
      961 aggcgcgaac gcatactccg cacccgaggc agagttcctc attcagtcgg gctttccgtg
     1021 ccttcgggcg gtggggatcg ttcgaggaca ccagtgtcat cgcctccacc gggcaggcat
     1081 tcacgcactt cccgcagccg gtgcacgcgg cctcgtccac ctccggcagg aagttcgttg
     1141 tgtggatcgg gttgaggatg gcgaaccgcc gcgccgcgat catcgcctcg cagcagcacc
     1201 cgc
//
LOCUS       DAOTNG010000496         1200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_94279_length_1200_cov_6.319651, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000496 DAOTNG010000000
VERSION     DAOTNG010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94279_length_1200_cov_6.319651"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="P5179_14740"
     CDS             complement(<1..367)
                     /locus_tag="P5179_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRS96525.1"
                     /translation="MFDPWVFTACGLIAGLYMAWTIGANDVANAMGTSVGSRVLTLRT
                     AILVAAVFEFSGAFFAGGQVTNTLRYDVINVHVVEKVGHTMGVGMLAALLASAAWLHL
                     ATWRGWPVSTTHSIVGAVAG"
     gene            complement(406..1080)
                     /locus_tag="P5179_14745"
     CDS             complement(406..1080)
                     /locus_tag="P5179_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HRS96526.1"
                     /translation="MARSILALFSRSPWGAIQEHIRSVNQCVQLLDPLMDAVMAGNHA
                     RTGELAKEISALEHAADEVKNELRDHLPRSIFLPVAREDLLEILSTQDSISDAAEDVG
                     MIVTMRKMEAQNDLRDLLDPLVQKTLESVSCYSQVIGEIDRLVDASFVGKEAQRVMDL
                     IRQVSTLEHEADLAQEALSRRLFEIEDRLKPGALFMWIEIIRRLGSVANKAEKAANRL
                     RMFLAT"
BASE COUNT          266 a          374 c          324 g          236 t
ORIGIN      
        1 aacctgccac agcgccgacg atcgaatgcg tggtcgatac cggccagcca cgccaggttg
       61 cgaggtgaag ccaggcggcc gacgcaagaa gcgccgccag cattcccacg cccatcgtat
      121 gcccgacctt ctccacaacg tgcacgttga tgacatcgta cctcagggta ttcgtcacct
      181 gtccacccgc gaaaaacgca ccggaaaatt cgaaaaccgc ggccaccaga atggccgtcc
      241 gaagcgtaag tactctcgac ccgacggatg tgcccatggc gttggcgaca tcgttcgcgc
      301 ctatggtcca cgccatgtac agtcccgcga tcagaccgca tgcggtgaaa acccaggggt
      361 caaacacgaa agctcccgaa cgagagagat ggttcgtaac ggacgttacg ttgcgagaaa
      421 catgcggagg cgattcgccg ctttttccgc cttgttggcg acactgccca gtcggcgaat
      481 gatttcaatc cacatgaaga gcgcaccggg cttgagacgg tcctcgattt caaaaagccg
      541 gcgtgaaagt gcctcctgcg cgaggtccgc ttcgtgttcc agcgtgctca cctgccgaat
      601 caggtccatc acacgctgcg cttccttgcc aacgaacgac gcatccacaa gacgatcgat
      661 ttcgcctatc acctgtgagt agcaggacac ggactcgagc gtcttctgaa caagcgggtc
      721 aagaaggtct cgcagatcgt tctgcgcctc cattttccgc atcgttacga tcattccaac
      781 gtcttccgcc gcatcggaga tgctgtcctg cgtggaaagg atttccaaca ggtcctcgcg
      841 cgcaacaggg agaaaaatgc ttcgcgggag atgatcgcgc agttcgtttt tcacctcgtc
      901 cgcggcgtgt tccagcgcgg atatctcttt cgcgagctct cccgtgcggg catgattgcc
      961 tgccatgaca gcatccatga gcgggtccag caactgcacg cactgattga ccgatcgaat
     1021 gtgttcctga atcgctcccc agggcgaacg ggagaataac gcgagaatag aacgtgccat
     1081 agcctttccc tccgaaactc cgaacccggt tgaacgccga attccaatgt acttcgcgca
     1141 ttgaggtgtc tcaaaccggc gtcgcgggaa agcgcatgga ccgattgccc gctttgcgct
//
LOCUS       DAOTNG010000497         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_95020_length_1194_cov_5.607550, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000497 DAOTNG010000000
VERSION     DAOTNG010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95020_length_1194_cov_5.607550"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="P5179_14750"
     CDS             complement(<1..994)
                     /locus_tag="P5179_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96527.1"
                     /translation="MLRTCVIGLGPIGNRHADIHQADPLATLVGVCDWNPERAEAAGK
                     RLGVPFFTDAAKMLESLRPDMVSVATGGHEYGSEHAAATMVALEHGCHVLCEKPISNE
                     LEPARKMVALAREKRLCFAVNLNHRFTPACRHARKWLAEGRIGIPRFMNMAMWIGNPR
                     ESSPWFHFKALHPHTIDIMRYFCGEIEAVHAFAIKAPGRSIWSTAQFNFRFKSGATGH
                     LTGSYDIMRGHPMERAEVGGTRGRILLEDMFKELTLFPHDSSEKIVVTNPIIGGMGDF
                     TDTFINRIHNFFVELTGGVPHDRIDGQAADALAAQAVIDANIRSMRTGKVERVDP"
     gene            complement(994..>1194)
                     /locus_tag="P5179_14755"
     CDS             complement(994..>1194)
                     /locus_tag="P5179_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96528.1"
                     /translation="VLTNSIFGGIPQLSETWFRRLHEFLTSVQKGNLARDTTAVDACA
                     AARVMEAAYHSAERGSVEEVES"
BASE COUNT          219 a          361 c          355 g          259 t
ORIGIN      
        1 cgggatccac ccgctcgacc ttcccggtcc gcatacttcg gatatttgcg tcaatcactg
       61 cctgtgcggc gagcgcgtct gccgcctgcc catctatcct gtcgtgtgga acgcctccgg
      121 tcagctccac gaagaagttg tggatgcggt tgataaacgt gtccgtgaag tcgcccatgc
      181 ccccgatgat cggatttgtc acgacgatct tctcgctcga atcgtgcggg aacagggtca
      241 gttctttgaa catgtcctcc agcagaatcc gcccgcgcgt gccgcccacc tcggcgcgtt
      301 ccatcgggtg ccctcgcatt atgtcgtaac tgccggtaag atgtcccgtt gctccggact
      361 tgaagcggaa attgaactga gcggtggacc agatgctgcg ccccggagcc ttgatggcaa
      421 atgcgtgcac tgcttcgatt tccccgcaga agtagcgcat aatgtctatc gtatgggggt
      481 gaagggcttt gaagtggaac cacgggctgc tttcgcgggg gttcccgatc cacatcgcca
      541 tgttcatgaa acgcgggatg ccgatgcgcc cttcggccag ccatttccgc gcgtggcggc
      601 acgcaggagt gaaacggtgg ttaagattca cggcgaaaca gagccgtttt tcgcgtgcca
      661 gcgcgaccat ctttcgcgcg ggctcgagtt cgttggaaat cggcttttcg cacagcacat
      721 ggcacccgtg ttcaagggcg accatcgtag cagccgcgtg ttcgcttccg tattcgtggc
      781 cacccgttgc cacgctcacc atgtccgggc gcaggctctc gagcattttc gccgcgtccg
      841 tgaagaatgg aacacccagc cgtttgccgg cggcctctgc tcgttcggga ttccagtcgc
      901 atacaccgac gagagttgcg agaggatcag cctgatggat gtcggcgtgt cggtttccga
      961 tggggccgag gcctatcacg caggttctca acatcaggat tccacctcct caaccgatcc
     1021 gcgttcagcg ctgtggtacg cagcctccat tacccgggcg gcggcacatg catcaacggc
     1081 ggtcgtgtcg cgagccagat ttcccttctg aacagatgta aggaactcgt gcagacgccg
     1141 gaaccaggtc tccgaaagct gggggatacc gccgaatatg gagttggtaa ggac
//
LOCUS       DAOTNG010000498         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_98215_length_1169_cov_4.213645, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000498 DAOTNG010000000
VERSION     DAOTNG010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98215_length_1169_cov_4.213645"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="P5179_14760"
     CDS             <1..984
                     /locus_tag="P5179_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96529.1"
                     /translation="NGCNMVNWYLQDWWREVEDDAAVRREQEALKEGPGLVCGLGGSP
                     YAKSYLDGPIPKLHSVHIANVGHNTVADIIKFREECPTRPQFMFLFAQISPGIWKQIE
                     SELDEYAAHPEIVLLTMDEFFLTLQDAIKKGLVKDSLYEITEPIAERWLKAPGRHRLP
                     ICEQVTRELADTAHAEPTERRLRLAETAWTELVSREVENVARDRERFTTHFKGRTPFA
                     EAEEADALHYTAFTVAWVVVRAAIQAQGVYANHRTQCLNDFKRLCGGYVDVKPFQRLF
                     DTWETWDDGVPDMTELTALCDALVTETRKLRDALGPDESEAAFTNWPPRTI"
BASE COUNT          250 a          340 c          363 g          216 t
ORIGIN      
        1 aatggctgca acatggtcaa ctggtatctg caagactggt ggcgtgaggt ggaagacgac
       61 gcggccgtgc ggcgcgagca ggaagcactg aaggagggtc ccgggctggt gtgcgggctg
      121 ggcgggtcgc cgtatgcgaa gagttatctc gatggcccga tcccgaaact ccattccgtg
      181 cacatcgcga acgtagggca caataccgtt gccgacatta tcaaattccg ggaagagtgt
      241 cccacccgac cgcaattcat gttcctattc gcccagattt cccctggcat ctggaaacag
      301 atagagtcgg aactggacga gtacgcggcg catccggaga tcgttctgct gacgatggac
      361 gaatttttcc tgaccctgca ggacgcgatc aagaagggcc tcgtgaagga ctcgctgtac
      421 gaaatcacgg agccgattgc ggaacgttgg ctgaaggcgc cgggccgaca cagactgccg
      481 atctgcgaac aggtgacgcg ggagctggcc gacaccgccc acgcggaacc aaccgagcgt
      541 cgcctgcgcc tcgcggagac agcgtggacg gaactcgtct cgcgcgaagt ggaaaatgtc
      601 gcccgtgatc gcgaacggtt cacgacgcat ttcaagggtc ggaccccttt cgcggaggcc
      661 gaggaagcgg acgcgcttca ttataccgcg ttcaccgtgg catgggtggt tgtcagggca
      721 gcgattcagg ctcaaggcgt ctacgcgaac caccgaacgc aatgcctgaa cgacttcaaa
      781 aggctctgcg gcggatacgt ggacgtgaag ccgttccagc gcctcttcga cacctgggaa
      841 acctgggacg atggcgttcc cgatatgacg gaactgacgg cgttgtgtga cgctctcgtg
      901 acggaaacac gaaaactacg ggacgcgctg gggccggacg aaagcgaggc ggcattcacc
      961 aattggccgc cccgaaccat ctgatctccc agaccagaaa gaagcggctc ggcatcctcg
     1021 aagatgccga gccgctcccg tttccgaaaa cctctccgga attacgcctt cgggtgctcg
     1081 atctctccca tcttgtaatc tttggggctg agcttctcga gtggttcctt cacgttgggg
     1141 cactcgataa cgccccgagc ttccgtgtt
//
LOCUS       DAOTNG010000499         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_104107_length_1127_cov_2.737873, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000499 DAOTNG010000000
VERSION     DAOTNG010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104107_length_1127_cov_2.737873"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..>1127)
                     /locus_tag="P5179_14765"
     CDS             complement(127..>1127)
                     /locus_tag="P5179_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS96530.1"
                     /translation="LMGFFPERWDELARRDGFVVVPLHTARTFEEFYAAFLRNLRSVI
                     GAHVAARNRTEAEGARYNPCPLHSATISDCIERARDMMAGGARYSFGSVSLAGIGTLI
                     DSLYAVKVVVFDQERLSLTRLRDILAADFEGEEALRQYIVNRIPKFGHGDPGMREFST
                     RVFADVARACSGYQNTRGGTYEASLFSFRSFVDFGRRGGATPDGRHAGEHLSPGMSPS
                     QLALGRECNVTQVLRALEPVDMTDYPVVAVLDVKMPLSPEQANPSVLESVVRCFVDYG
                     GSVLQINVVDPSALVDAREHPERHPDLVVRVSGYSAYFRTLSPDIQQEVVARALAR"
BASE COUNT          226 a          347 c          351 g          203 t
ORIGIN      
        1 ccccgccgcc ggtgaagccg atgcagtagg gcacgcgttc cgtttccttg tgcgcgatgg
       61 ccgctttgac gatttctcgc cgggtcatgg agtgcagtcc tcaggattgg gggtgcgggg
      121 gaaagctcat cgtgcgaggg cccttgccac cacttcctgc tggatatccg gggaaagggt
      181 ccggaaatag gcgctgtacc cggagacgcg cacgacgagg tccggatgcc gttcgggatg
      241 ttcacgggcg tccaccagcg cggaaggatc cacaacgttg atctgaagga cggaaccgcc
      301 atagtccacg aaacagcgca ctacgctctc cagcacggat ggattggcct gttcgggcga
      361 caaaggcatc ttcacgtcga gtacggcgac cacggggtag tcggtcatgt ccaccggctc
      421 aagcgctctc aaaacctgcg tgacgttaca ttcgcggccg agtgcgagct gactcgggct
      481 cattccgggg gaaaggtgct ctcccgcgtg ccgcccatcc ggcgttgcgc cgccacgacg
      541 gccgaaatcc acaaatgacc ggaacgagaa gaggctggcc tcgtacgtgc caccacgggt
      601 attctggtag ccgctgcatg cgcgtgcgac gtcggcaaaa acgcgcgtgg agaattcgcg
      661 catccccgga tcaccgtgtc cgaatttcgg gattcgattg acgatgtact gccggagcgc
      721 ttcttcgccc tcgaaatccg ccgccaggat gtcgcgaagg cgggtgagag aaagcctttc
      781 ctggtcgaat acaaccactt tcacggcgta caaggagtcg atcagcgtgc caatccccgc
      841 cagtgaaacg cttccaaagc tgtatcgcgc gccgcccgcc atcatatcgc gggcgcgttc
      901 gatacaatcg ctgatggttg ccgagtggag ggggcatggg ttgtaccgcg cgccttccgc
      961 ctctgtgcgg ttacgcgcgg caacatgagc gccgatgacc gaccgcagat tccgcagaaa
     1021 tgcggcgtag aactcctcaa acgtccgtgc tgtgtgcaac ggcacgacca caaacccgtc
     1081 acgcctggca agttcgtccc agcgttccgg gaaaaagccc atgagaa
//
LOCUS       DAOTNG010000500         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_114387_length_1064_cov_3.146680, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000500 DAOTNG010000000
VERSION     DAOTNG010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114387_length_1064_cov_3.146680"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="P5179_14770"
     CDS             complement(<1..247)
                     /locus_tag="P5179_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96531.1"
                     /translation="MPTTVGRIEETVLEKYGDFHNGAGPMWCFGSHTIVRSGDTVYAS
                     MVDVEPEARPLCNAKWQLWQRQDGGSWGKIAEQQKAGE"
     gene            complement(278..>1064)
                     /locus_tag="P5179_14775"
     CDS             complement(278..>1064)
                     /locus_tag="P5179_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS96532.1"
                     /translation="ALLDDWLKNRLLAARLREHRIIAMLRDDATESGVLRAIESLDSA
                     IRSLPEASDEVLRLEKTLLQAGPRMPVGRVSHATGNLAWMKATLKKALVDPSVRSALV
                     ADVIDYERGVYYVDCGNPVRDTFCVHGRRYTCLDERSYQTPFSEAPEIRPSQNSLVMT
                     CGEDDGIVYRFPDLHPDRKYVVRLTYFLPTLFTYRETRSQKLEANGVEVHGPLVLPRK
                     RPETFEFALPRASYSAGETTLRFRKAEGGVTTVVSEIWIRECD"
BASE COUNT          218 a          337 c          278 g          231 t
ORIGIN      
        1 gttcaccggc tttctgttgt tctgctattt ttccccatga accgccgtcc tggcgctgcc
       61 acaattgcca tttggcgttg cacaggggac gtgcttccgg ctccacatcc accatgcttg
      121 catacacggt atctcccgac ctgacgatcg tgtgagaacc gaaacaccac attggcccgg
      181 cgccgttgtg gaagtcaccg tacttctcaa ggacagtctc ttcaatacga ccaacagtgg
      241 tcggcatggg aaccctcgtg attggttttc gccgatttca atcgcactcg cgtatccaga
      301 tctcggaaac aactgtcgta actccaccct cggccttcct gaaacgcagg gtcgtctccc
      361 ccgctgaata cgaggcccga ggtaacgcaa actcgaatgt ttccggacgt tttcgtggaa
      421 gaaccaacgg accgtgtacc tccacgccgt tggcctcaag tttctggctc cgcgtctctc
      481 gatacgtgaa gagagtggga aggaaatacg tgaggcgcac cacgtacttc cgatccgggt
      541 gcagatcagg gaatctatac acgatgccgt cgtcctcacc acacgtcatg acgagggagt
      601 tctgggatgg tcgtatttcc ggtgcttcag agaacggtgt ctggtaggat cgttcatcca
      661 gacaggtgta cctccgcccg tgcacgcaaa acgtgtcccg aacggggttt ccgcaatcca
      721 cgtagtaaac cccgcgttca tagtcgatca cgtccgccac cagcgccgaa cgaactgaag
      781 gatccacaag cgcctttttg agagtcgcct tcatccaggc gagattgccc gtcgcatgac
      841 tgacccgccc gaccggcatt ctcggccccg cctgaagcag tgttttctcc aggcgcagga
      901 cctcatccga agcctcgggg agggaacgta tcgcgctatc gagtgactcg atcgcgcgca
      961 gcaccccact ctccgtagcg tcgtcccgga gcatcgctat gatccgatgt tcccgcagcc
     1021 tcgcggcaag caggcgattt ttcagccaat cgtccagaag cgcg
//
LOCUS       DAOTNG010000501         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2901 NODE_126026_length_1004_cov_5.564805, whole genome
            shotgun sequence.
ACCESSION   DAOTNG010000501 DAOTNG010000000
VERSION     DAOTNG010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867925
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:09:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,956
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126026_length_1004_cov_5.564805"
                     /isolate="AMR_MDS_2901"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1004
                     /locus_tag="P5179_14780"
     CDS             20..>1004
                     /locus_tag="P5179_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS96533.1"
                     /translation="MLGAGGIAGWWIRNAFPRFVDRMTVVGLVDIRENVLHESGNLLG
                     IPESARFTDMEEAFAKTEADFCAIVTPPWVHEQAVMLAVGKRMAILSEKPIADSWKAC
                     TAIYRAVAAAGLKMEVIQNYRYEPTMLTVRHILRSGRLGRVNYIVGRFADDYRRYGSW
                     GQFRHEIPHSLLVEGGVHHLDMLRNLSGGDTALISGSEWNPPWSSFKGESNALFTLTM
                     TNSVRAQYEGSCNEAGVVNGWHREYYRVECEYGSVTVDSDLVVRIWERSEGRSCKMTE
                     VPLLNPTYAGHLWQLDEFLSWLDGGREPDTVLSDNMQSAATMFAAIVASGDA"
BASE COUNT          212 a          249 c          338 g          205 t
ORIGIN      
        1 ggaaaagaag acctgtctca tgctgggcgc cggtgggata gccggttggt ggattcgtaa
       61 cgcgttcccg cgatttgtgg atcgaatgac ggtcgtcggg ctggttgata tccgtgaaaa
      121 tgtactgcac gaatcgggaa acctcctcgg aatccctgaa agtgcccgtt tcacggacat
      181 ggaggaggca ttcgcgaaga cggaggcgga tttctgtgca atcgttacgc cgccgtgggt
      241 gcacgagcag gcggtgatgc tggcagtagg aaagcggatg gcgatattga gcgagaagcc
      301 gattgccgat tcgtggaagg cttgcacggc catctaccgc gcggttgcgg cggcggggct
      361 caagatggaa gtgatccaga actatcggta tgaacccact atgttgacgg ttcgacacat
      421 ccttcgcagt gggcggctgg gacgtgtcaa ttacatcgtc ggccggttcg cagatgatta
      481 ccggaggtac ggttcctggg ggcaattccg gcacgaaatc ccccacagcc ttctcgtgga
      541 gggcggagtg caccatctgg acatgctgcg gaatctttcg gggggcgaca cggcgctgat
      601 aagtggctcg gaatggaatc cgccctggtc aagtttcaaa ggggagtcca acgcgctgtt
      661 tactctcaca atgaccaact ctgttcgcgc ccagtacgaa gggagctgca acgaggcggg
      721 cgtggtgaac gggtggcaca gggagtatta ccgcgtggaa tgcgagtatg gctccgtgac
      781 ggtggacagt gacctcgtgg tgcgaatctg ggagcgttcc gagggaaggt cctgcaagat
      841 gacggaagtg cctttgctga accccacgta tgccggccac ctctggcaac tggacgagtt
      901 tctatcctgg ctggacggag gcagagaacc cgacactgtg ctttcagaca acatgcagag
      961 cgcagcaaca atgtttgcgg caatcgtcgc ttcgggtgac gctg
//
